LOCUS       DAORKW010000001        30220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2_length_30220_cov_5.352528, whole genome shotgun sequence.
ACCESSION   DAORKW010000001 DAORKW010000000
VERSION     DAORKW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 30220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30220
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2_length_30220_cov_5.352528"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PLB46_00005"
     CDS             complement(<1..982)
                     /locus_tag="PLB46_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF74919.1"
                     /translation="MRKYLLLIMISFVSMQLFGQGRTITGKITDADNGEYMPGVTILL
                     EGTQNGTISDFDGKYNINVPESGGTLVFSFVGYETQKITLGEKNVVYVSLKMSQEILE
                     EVVVVGYGTEVKTKVTGSISEIKSDEIALTPVSTVEQTLQGRAAGLFIESNNGKVGGD
                     VKIRIRGSSSINASNQPLIIVDGIPINTVAINDYFNVYLNPLNDLDFNDIASVEVLKD
                     ASASAIYGSRAANGVLIITTKRGKEGKAKITLDYQQGYSEPTRLREFLNAEEYIDYFT
                     QAAINAGEYDFANGLSGYATVEEAVEDYIGDVEKKFDRLAGHTDWRTDYAN"
     gene            1112..3229
                     /locus_tag="PLB46_00010"
     CDS             1112..3229
                     /locus_tag="PLB46_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRF74920.1"
                     /translation="MKKQKFIISMLFLAVSAVTALAQTQVPVAPVAEKIPYVIQGSQG
                     PRQDPYYWMKDRENPKVMEHLKAENAYLEAVMEPTKALQEQIFKELVGRIEEDESSVP
                     YKLGDYYYYSRYEKGGEYAIYCRKKGDLSATEEIIVNGNDLAVGNEYLSFYIEMSHDH
                     KMAAVIMDVAGRNFYTIKLKDLSTGEWLKDEIKDTRNGCIWTTDNKSLVYGVPDKKTL
                     RVYQIKKHVLGDKSKNDVVLLQEDDQTLDLYAYESRSKEYIFAFASRTDAGIIKYTKA
                     NQPDVFTTIEPMTTDHYYTVDHIKGDEFVVRTNYQAKNYRLCKTKIAAPTKENWADII
                     PARDNVFLETVEFFKDYYIAQESTNGLTTLRVIAPSGQNDYIKFDEPAYYATLGYNPD
                     PNTNDVRFVYSSLITPYSTYEYNVTTKAKKVLKQDIIPSGYDKSQYVTERIMVTARDG
                     KKVPMTIAYKKDTFKKDGSAPGYIYGYGAYGYSNEDYFDSNILSLMDRGFVYANTHIR
                     GGQEMGGSWYDDGKMMNKKNSFYDFIDCSKYLIQEGYVGKQKLFANGGSAGGLLMGAV
                     SNMAPELYLGIIADVPFVDVITTMEDETIPLTTFEWLEWGNPKNKAEYDYMMSYSPYD
                     NVEAKNYPNMLVTTGFNDSQVQYFEPAKWVAKLRATKTDNNLLLFKINLDAGHGGQSG
                     RYESLNETAFMYTFMLHCLGVTK"
     gene            complement(3308..5617)
                     /locus_tag="PLB46_00015"
     CDS             complement(3308..5617)
                     /locus_tag="PLB46_00015"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HRF74921.1"
                     /translation="MKKWKLLLIGLLCVSTQLLEAQQRVLPGHVEKKYQAIIKPGATQ
                     DWIEFKSDAKLNAASVFTEAPDLLGLTERDELRLMKTNIDEAGNKHYKFAQYYNGVRI
                     EGIEQLSHERNGKIHLINGDFIPGLNVNVIPTISAQQAIEIALAAYPAEKYMWEDEKK
                     EAAFKAKKKDPNATLYPTPELLLVKINPKEKNAADNYVLAYRMYVFATLPSIAKYVYV
                     NANTGEIIRDRSLEHFCNSTSVVTTFNGTKTVYTDYRTEDCGPYGDTETSYFPIDDCN
                     PDTEIRSYFSGDYGTPSYGEDWYYCSSTNTWEGFGNYTMVMTSLWGVRQAFEYYLNTY
                     GHESFDGSGGDIDIFNNKTYFDDDDNPNCTNANYTNIIDNLNFGSGNDCVAGTTDDYN
                     AIDIIGHEFTHGVIEYAHFDALDYSEESGALNESFADIFGEMVEFYVEGDGSPTWLHG
                     EDRGANRSFINPNDKGDPDTYFGDYWADLGGGDNGGVHTNSGVQNHIFYLLSEGGDGV
                     NDYGLEYHVDAIGYDKARRIAWGAMMNYLDGSDGYIIARNAWIQSAIDYYGSCSDEVI
                     AVGQAFQAVGVTKFTSYDLASICGTYILTGYSDATYGIENKSMLLGEFLVECSSTILS
                     PAVVTFESANYITLHPGFEALSGCTLTAWIDECEISDYNPDDLRFAADNVEDEELSAN
                     KPTTFDIYPSPASTSVSIDFNLEKEATGNLFVTDLSGKIIANWLTATTVTAGENTLTF
                     DVLPFASGMYVAIFQTADGIQTAKFVVQH"
     gene            complement(5646..7388)
                     /locus_tag="PLB46_00020"
     CDS             complement(5646..7388)
                     /locus_tag="PLB46_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74922.1"
                     /translation="MKKQLLLFAAALIAGATMVKAQTNTYPLTGSAGLGTTTPTASAL
                     LDMTSTSQGLLAPRMTKAQRDAIVAPATGLLIFQTNSTPGFYYYTGSSWAAMSSKGAN
                     TSLSNLAAAGTSINVGLTPATNNTLDLGSSTNNWNEVYVNSIKFMDGTTQSTATVGGG
                     GGGVGGSGTANYIPKFTAATTVGNSSIYDNGGNVGINTTTMIGSANFVAKSNNVTSYG
                     GMYIDMAGTSGRKPFYGYAVGGSAKAWTYYDEATSQFRLYNGGDQFVVDNTGQIGIGT
                     ITPSAKLNVETTGTTAVMKVTKPWTGVGATNFNLVEISNTYTFGYGTGLQASGGQIGV
                     KGVASNGAETGYGVYGSGFGAAGTTYGVYGTAGSTAGLSIGVYGTATSGTSQWAGYFV
                     GRGYFSGNLGVGAATPAYKVQVMGGTDAELTSGGFIVSGDATSLNIAIDNNEIMARNN
                     GAASTLYLNNDGGNVSMCDVGGSVMIGTSVPATGYLLSVDGKVMCEELKVQMSGSWPD
                     YVFADNYALPSLYELEASIKQNKHLPGIPSADVVEAEGIEVGTMQVQMMEKIEELTLY
                     IIELQKQIDALKAE"
     gene            7614..9047
                     /locus_tag="PLB46_00025"
     CDS             7614..9047
                     /locus_tag="PLB46_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74923.1"
                     /translation="MENTYLQQVLQVLSKAEWRDFGDFVASPYFNKNEKFVRFYQFLQ
                     PFYPKFDITGSSKEQLYIHATSADKYSDAGYRNLCSDLLVLVQEFLAQESLNAGTTIK
                     DSLINRTLILRGVLDLAERNVKKAETMVAKRDYDFQEQLMAAIWINDLRILHNIYNNR
                     KSMNASNPIMLEDNTHKFITEWALLRIFSSLMNNIKATRSYNLPLNTQKYQAYLNLYE
                     SFEPFNNKESRIMYLTVKMNVLNDEQAYFECFEILKTLDFTKNIRTNENLVIGLLDFA
                     LHKLNEDEKWRIELHELYDLKLSNKLWSEKKMLSYTSLFNAVHNSLQLNKISYAETIL
                     DTYIQDVSPTIATTIDNLCRAWIFFFKGDYDKAHEYLVKVETENILVKFEMRSLQCLI
                     YLQKKEWELLNAALESFRQFNAYNKSSINELVTLQFTIFCKYISSIAKLHPDYLEKDK
                     IKIQDQIKSEKNSYMRNWMLEKINSGK"
     gene            complement(9048..9914)
                     /locus_tag="PLB46_00030"
     CDS             complement(9048..9914)
                     /locus_tag="PLB46_00030"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRF74924.1"
                     /translation="MKRISLVAAVCALLFVACTSPESKETSNTKTDYPKGKLFIIGGG
                     DRGDSLMMDLIVASGWKPGELITTITLPSGWGDSAYIWLNNDFKKLTGQNCVKFDSAA
                     VNVPEKIDSLRKSKIIFISGGDQAVMMRLIKGTTIKAAINEAYQNGALISGTSAGASI
                     MSERMITGEGLVDTMYESTFDNIWKGNLELVEGLGLLDSVIIDQHFVARSRYNRLLCA
                     TIENKGFQCIGIDEGTAILVSQGMAKVVGVSQVVEVGAPIKVDMSRDTLLTARQIHLN
                     IYAPGEEFPIKQ"
     gene            complement(9962..11206)
                     /locus_tag="PLB46_00035"
     CDS             complement(9962..11206)
                     /locus_tag="PLB46_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF74925.1"
                     /translation="MTNAAYISIVAGMSERITSLRTTDEAMLYFAEQFNLLFHLDYCS
                     LYRVSEKKDVFIRSAECKIKKKSVPIAKPENRIQLPVSILDAKDKYVFINQDDRPFLF
                     GKKIEQLAAEVIVTIRRAGNIELIFYGTSFTPIEEDAFQNDTCRIFSNFFWQVEDALL
                     IRSKINDKQKELSGHIAENNDTLFQLNEKLITYNQELQQFAYSASHDLQEPLRTIASY
                     INLFLKSYGATLTEEGKEYLQFASDGAQRMHHLIKDLLTYSKLDFQSEPMTHFEGNAA
                     IRQALDNLQMSVEESNALILYPDLPVIYGNHSQIILLFQNLIDNAIKFRSKNEPLVFI
                     DLEELTDRWEFKVKDNGIGIAQQFQQKIFGIFNRLHGRDEIQGSGLGLSICKKIVERH
                     RGEIAVESKPGKGTTFIFSIGK"
     gene            complement(11282..11944)
                     /locus_tag="PLB46_00040"
     CDS             complement(11282..11944)
                     /locus_tag="PLB46_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRF74926.1"
                     /translation="MNEYPFIPFNPVRFSEHEMVIRSNQFYELMQKRRSLRFFSDEPI
                     PEIVLSNIIMTAGTAPSGANKQPWSICVVTNPKLKHEIRMAAEKEEQLSYSERMPEEW
                     LADLAPLGTDWHKPFLETSPALIVVFKRNYEVVDGRKKNNYYVTESVGIAVGMLLAAI
                     QNAGLCALTHTPSPMGFLKNLLQRPENETPFLLIPIGYPAADATVPDIERKAAKDFVF
                     LY"
     gene            11998..12612
                     /locus_tag="PLB46_00045"
     CDS             11998..12612
                     /locus_tag="PLB46_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRF74927.1"
                     /translation="MKVITAYQTIQSPSEGIYTEAGSRFLGYLYPLTTDEEVKQIRDN
                     LKKEHHKAVHVAWACRLGILPDTERSSDDGEPSGSAGKPILNELKSNNLTQVGLFVVR
                     YYGGKKLGIPGLIHAYGAAAAYSITSAKVETIPIKECYKISCKETDLHLVIHEVNKIG
                     AKIVSTEFGEICNFIITFNRDKHDKVIGKLETLWQIELAYSHSF"
     gene            12545..14326
                     /locus_tag="PLB46_00050"
     CDS             12545..14326
                     /locus_tag="PLB46_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74928.1"
                     /translation="MIKLLANWKRFGKLNWHIVIAFSFCSCFTQTIFAQNVTTPDIQL
                     AENFIASRLNYDELYLSTFVCVATRQSPYSTYYTFQQYFNHIPIENKLLKVGIDDKHQ
                     LTFFAGDYQTTTGWTNRAMVAKKLNTAYLLPNGKLINANTPLQPLIRVVENQPIYVYT
                     YDLQNEDRSRYTIVVNENDEVIEVQDHKVFFAGEDSTVLAKVFLPDPLTSAEVTYGAP
                     FVDDNDSDIAELNNEAAIVTMKVLFEDGVFYLKNDNIIIKDINVPTAAVVTSIIPEFN
                     YTRSESGFEDVNTFYHLTNFCNDIVAAGYTSLQDFYIEIDPHGASGADQSFYVSAAIP
                     TIQFGEGGVDDAEDADVIVHEFSHALSDHASPLSNSGLERRAIDEGYGDYFAASYSRK
                     FSDYNWEKIFSWDGHNEFWFGRNANTTKHYPEDNSDNIYAASEIWSGALMDIFDVIEK
                     TNCDQLVYEALFGSFPNMTMPDGASLILNAEATIFGGLYYEQVYDALFARGLIYPNAV
                     TNITNNKIQILNTNGFAFFDEPLLIQFNQYTDVQLNCYSVDGKLLAHQQTSGATISWK
                     PEITQQGLFIIEVVTPTGTIATKISHY"
     gene            complement(14327..15187)
                     /locus_tag="PLB46_00055"
     CDS             complement(14327..15187)
                     /locus_tag="PLB46_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF74929.1"
                     /translation="MSEQLKQIFVPTDFSSASVAALQYAIYIGNRTNSHIHLVHFALR
                     ESLKEKNDTASAYKAKIRALEARLDEMKNTSGAAFRITTEVITTNDPIPGLIHDYGLK
                     IGAELACIGTFGSDSKPNKFNLGNNTEKLVKSVDFPVLTCRDVKSPIQFKNFLLPIDL
                     TKYTSEKVERIIRFAKGFDATIHLVAVSEFLEEFISSKTALEEKMEEAADTIRNHGLK
                     CTTEMIRHDMVSNSILLYADEIDADLLVIMSHAENKFNQLIFGSRINKVISHSTIPVL
                     SFRPEDEDSE"
     gene            15236..16108
                     /locus_tag="PLB46_00060"
     CDS             15236..16108
                     /locus_tag="PLB46_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HRF74930.1"
                     /translation="MSTTSSSELILNSDGSVYHLHLLPEQVADTVITVGDPDRVAMVS
                     KHFDRIEHRVQKREFITHTGYIGHKRITVISTGIGPDNIDIVFNELNILRNYNFNTQS
                     FNAIANPLQIIRFGTSGAIQADIPVNSLLASGFGIGIDNVLHFYQFENTKEEAAICEQ
                     FKLHCYFNHTIHPYVFSADTHLLAQLDNTFIKGITLTAPGFYAPQGRAVGYALTHPEF
                     YNDLRSFRHLDWRITNIEMETATMFGLSRVLQHQCLSLNVLLANRATGTFSTDPTAAI
                     ENLIHHGLTFIAGL"
     gene            complement(16110..16880)
                     /locus_tag="PLB46_00065"
     CDS             complement(16110..16880)
                     /locus_tag="PLB46_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HRF74931.1"
                     /translation="MDNKYIISLVSYLNTKPFLYGLENAAIKEKLDLQLDIPSVGGKK
                     ILSGSVDIGLVPVAVLAKLNDARIITDYCIGADGPVKSVSIFSQQPIDKIEKVFLDYH
                     SLTSVQLVKILLREYWQVNPIQIAAETGYIDQIKGTTAGLVIGDRALALNKKFPYVYD
                     LSDAWKKMTGLPFVFAAWITRKTIPEDIISEINEALKFGVQHIDLVAKNYTHPNFTIA
                     EVQDYLQNDINYNLDTTKRRALDLFISKVRANTNIEVE"
     gene            complement(16873..17481)
                     /locus_tag="PLB46_00070"
     CDS             complement(16873..17481)
                     /locus_tag="PLB46_00070"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRF74932.1"
                     /translation="MEKEIFIIRHGETDFNQLGIVQGKGVNSSINANGTQQAQAFFEA
                     YRNENFEKIYISQLQRTAQTVAPFRELNIPVEIHEGLDEISWGIHEGKKDGDTFKDFY
                     RILHLWQAGETTVKIDEGESPEEVQFRQQKFVKMLEATKEKKILICSHGRAIRILLCT
                     MLNQPLKDMDTFPHHNVSLYKLHYNANGFKIVLFNDIGHLNG"
     gene            complement(17483..18682)
                     /gene="mqnE"
                     /locus_tag="PLB46_00075"
     CDS             complement(17483..18682)
                     /gene="mqnE"
                     /locus_tag="PLB46_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HRF74933.1"
                     /translation="MTREDDIQQLIETTTTNEQLRSIAKKVLAGQRISFDEGVTLYEQ
                     ASLSFVGTLADYIRTKKHGSVTYFNRNFHIEPTNLCVFDCKFCSYSRLLKQRSEGWVM
                     SSDEMLDIVRSYQNKPVTEVHVVGGVLPELNLRWFADIIRDIKILRPDLHVKGFTAVE
                     LEYMIRKAKMSIREGLQFLKDAGLGSLPGGGAEIFDETVRSQICADKCDSATWLEIHK
                     TAHELGLPSNATILYGHVESYTNRVDHMERLRKLQDETGGFNCFIPLKFRNKNNQMEH
                     VQEVSVIEDLRNYAIARIYLDNFPHIKAYWPMIGRQNAQLSLNFGVNDIDGTIDDTTK
                     IYSMAGAEEQSPVMTTKEICELILTAGKIPVERDTVYHTIKNYANVDLDAEFGVDHFV
                     NLPIINN"
     gene            complement(18766..20061)
                     /locus_tag="PLB46_00080"
     CDS             complement(18766..20061)
                     /locus_tag="PLB46_00080"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRF74934.1"
                     /translation="MKKALFAGLLLALSTVSFGQTVVRCGSNTYLKQLETNFPEEFAA
                     IKEAAIKRDANKVSTTYKTNGGVYTIPVVVHVVYSAASQNIDDEFILSQIDVLNADYR
                     RLNDDADETPAAFEGLAADAQIEFCLAQQDPDGVAHSGITRTETDISSWSLFVGVGAD
                     NYSDNVKFTDKGGEDAWPKTDYLNIWVCNLTGGVLGYATPPGGPSGKDGVVIGYKYFG
                     DGSPGGVYNKGRTATHEVGHWLGLNHTWGDDDFAAEPQCGGSDGISDTPNQEDATYGN
                     PSFPRLDDCSPSSPGIMFMNYMDYVDDGAMNMFSEDQAAEMRDVIENDRESLLTSQGC
                     VQGGVDVNEVLKNQLIQLSIYPNPSSGEFVFEMKNFVHNELTIEVYNLTGQLIDQIIT
                     TNKGEEHVVFDASNLSAGDYMVKASDGEFYLTQKITVIK"
     gene            complement(20193..21431)
                     /locus_tag="PLB46_00085"
     CDS             complement(20193..21431)
                     /locus_tag="PLB46_00085"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRF74935.1"
                     /translation="MRAGIYLILILVCCSSNLLFAQEKCGTSAILEQMQTTNPEIYAK
                     IKERSKTQVNAKITDDIYVIPVVVHIVYNTEAENIDDTRVYTQINVLNEDFRRLNADT
                     IKTLPGFESVAADVGIEFCLAFYDPNGDSTTGIIRTYTDTTEWLLSFSEEVKETSQMG
                     DDAWPSSAYLNIWVCDLQDGILGYAVSPGASPEVDGVVIDYKNFGIADVSSKPYDLGR
                     TCTHEVGHWLGLTHVWGDDGGTCAGSDGINDTPDQAEATYGCPNTVLVDACSPTAPGI
                     IYQNYMDYTDDACMNLFTEDQKTRMRSVMETYRASLLTSAAGCNEIGPLPGDIAEILV
                     YPVPTSGQFTVSIKNFLGYQDNFNIAVYNSLGQIIASANPSAANNVAQYFDLSYLASG
                     CYIVQVFNGTYFLTQQFMVY"
     gene            complement(21453..22511)
                     /locus_tag="PLB46_00090"
     CDS             complement(21453..22511)
                     /locus_tag="PLB46_00090"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRF74936.1"
                     /translation="MQEFDDDLEIGDLGAESDEELYEHYRVVTDRKQEPLRIDKFLFN
                     RIENISRTRIQSAAEAGCILVNQKPVKSNYKIKPFDEIVVVLPQKPAKYDVRAENIPI
                     DIVYEDDDVVLINKKPGMVVHPGLGNFSGTLVNALLYHFEQLPPKQDEPYRPGLVHRI
                     DKFTSGLIIAAKNEFAMAHLAKQVVDHTINRRYNALVWGEFDEQEGTVVGNIARHQRY
                     RKLFTVYPPGGEIGKHAVTHYKVLESFGHTSLIECKLETGRTHQIRVHMQHIGHPVFS
                     DNTYGGDKIVRGTIYTKYRQFVDNCFALIPRQALHARSLGFIHPKTGKEMYFETELPD
                     DFLAATEKWRAYSKHIKP"
     gene            complement(22511..23209)
                     /gene="lipB"
                     /locus_tag="PLB46_00095"
     CDS             complement(22511..23209)
                     /gene="lipB"
                     /locus_tag="PLB46_00095"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRF74937.1"
                     /translation="MQLVHFRDLGKMEYGYAWKLQESIFNDIVQKKLKNRVLNDEEQL
                     TYSHQLLFVEHDPVITLGKSANENNVLLNETLLNQHGVSLFHINRGGDVTYHGPGQIV
                     GYPILDLDYFFTDLGKYLRSIEEVIILTLADYGLQGERLPGATGVWLDATNKNARKIC
                     AIGIRSSRWVTMHGFAFNVNTDLNNFKLIIPCGIQDKAVTSLEKELGKQIPLDEVKAK
                     LKQHFATVFNCKLI"
     gene            complement(23209..24192)
                     /locus_tag="PLB46_00100"
     CDS             complement(23209..24192)
                     /locus_tag="PLB46_00100"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HRF74938.1"
                     /translation="MDKYIDRFPQQLLEAVEIGLAASDFKLQNPLHHIVISGMGGSGI
                     GGMLIKDILKQRIKTPIEIINDYTLPLHCGENTLVICSSYSGNTEETLATFDQALRKK
                     CMIACVTTGGKLLEKAIKNNLPHIIIPGGMPPRSCLGYSTVAQLFVLKCAGIINDYFI
                     EEIKEAAHLLINKRDDIQLTAKTLADQLQQTIPIIYGDATLQAVVLRWKQQINENAKM
                     HCFCHVIPEMNHNELVAFHTKNSAIAAVFLRHGNEHPQTTRRFELVNELISDKVGCTV
                     VVGSAGNHTIENMFYLIHLGDWMSLYLSILKGVDPIKIDMLDWLKTALEKK"
     gene            24219..24584
                     /locus_tag="PLB46_00105"
     CDS             24219..24584
                     /locus_tag="PLB46_00105"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRF74939.1"
                     /translation="MGRHNELGRLGEEKAAEFLENKGYTILAKNYRFGRAEVDIIASN
                     SQHVLFVEVKTRTNYLFGYPEEAVGAKKQRLMSRAASEFMFQHQLNLPLRFDVIAIFK
                     TKINEWQIQHLEDAFFNYG"
     gene            24581..24964
                     /locus_tag="PLB46_00110"
     CDS             24581..24964
                     /locus_tag="PLB46_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF74940.1"
                     /translation="MITGVNHIAIICSDYHISKHFYHEILGFEIIAENYRAARNSWKL
                     DLRVNEYTAIELFSFEDAPPRLSYPEARGLRHICFSVKDLDAATKKLSALGITFEAIR
                     IDEYTGKRFVFFADPDDLPIELYEQ"
     gene            complement(25021..25536)
                     /locus_tag="PLB46_00115"
     CDS             complement(25021..25536)
                     /locus_tag="PLB46_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF74941.1"
                     /translation="MTNTEFESHIIQLKSPLKAFALNLTRDRDDALDLVQETYMRALS
                     NQDKFNDGTNLKAWLLTIMKNIFINNYRKASKRNTVSDTSENLFLLNSSVGAIANQAE
                     NDFVMKDLIKAINQLDEDYKKPFIMHYQGYKYEEIADVMNLPLGTVKSRIFFARKQMK
                     DFLENKGFDRY"
     gene            complement(25629..26522)
                     /locus_tag="PLB46_00120"
     CDS             complement(25629..26522)
                     /locus_tag="PLB46_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRF74942.1"
                     /translation="MAAIYSIRDLESLSGIKAHTIRIWEQRYGILNASRTETNIRYYS
                     EAELKYLMSLALLNRNGYKISKLAKMTHDQVSGLVTAMIQAPNEFNNQIEGLTIATIA
                     FDEEKFEKILNTCILQLGFEDAIKKVVFPYLQKLGMLWVSGTIIAAQEHFMTQLLRQK
                     LLVAIDGQTTKYDDQSREFVLFLPNGEWHELSLLFLSYLLKSRNHKVVYLGPSVPLND
                     VIQVGETLQPDGFYTIITTNPTAFTVSDYLNKLADKFPESKVFVSGSQTIGPIKGLSA
                     NVYVIESLETVVAQLENLAVA"
     gene            26656..27516
                     /locus_tag="PLB46_00125"
     CDS             26656..27516
                     /locus_tag="PLB46_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF74943.1"
                     /translation="MKKVLIPTDLSENAAQTLSYLLPLVIAQNAEVHLVHAVDDPFVK
                     EERSDFDGTGVEKYTTDILFKMRAEADADMEKFATLFRESLAQAGKDLPVYSTVENGL
                     PDEIILSKAAAIQPQLIILGRHHHNRMDRLFFGSVTQSVMQKTHFPVLILPQHFEYAQ
                     PKEILYMSDMDDHDIISIGKLINLLQPYSFNLHIVHFNINEIGTEEKLFALGEKIKND
                     HHEIPISYETVDGEILRVAWQQYIQKRGIDLIALTAHKHNGLQRLLNKDTAVDVLYHS
                     NLPLMVMHKY"
     gene            complement(27513..28265)
                     /locus_tag="PLB46_00130"
     CDS             complement(27513..28265)
                     /locus_tag="PLB46_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF74944.1"
                     /translation="MKQCISAFFSFWLFAGTAFGQLIPAEDWTQTDCNGVEHNLFSEL
                     DAGEVVLMEIVMLDGCMPCINAAHYMAPVIEYYNANYDNRINYYTFGFDDSYSCGQLI
                     DWKTENSIICNAQFVEGEAIAANYGGMGMPTIIVAGRSSHVVCYNKFGFGPLDTANLA
                     NAFEYALGLTETLAIENPEKSMLVYPNPVHDVLHFDMNGTADISIKNLAGVTIMQQTG
                     TNHIDVNSLPAGLYFYEINAATFSGSGNFIKN"
     gene            complement(28275..29483)
                     /locus_tag="PLB46_00135"
     CDS             complement(28275..29483)
                     /locus_tag="PLB46_00135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRF74945.1"
                     /translation="MKFYKNIILKSLQPMQKGYLHITLADGSTMELGNKSNQHHADVH
                     VHDDNFFKRAFLFGDIGFAEAYMACEWTTSNLTALLSWFLLNVDTAPTLSGSTAKTLI
                     LNAFQFANKIFHSKRKNSVTGSKKNIAAHYDLSNEFFSLFLDKTMTYSSAYYTAPKQE
                     LEKAQFEKYESLCQSLKLNQDDHLLEIGTGWGGFAMYAAQTYKCKITTVTISKQQFDF
                     ATQRIKQEGLEHLIEVQLKDYRLIEGKYDKVVSIEMIEAVGAQYLKVYFDKIQSVLKP
                     SGVLAIQAIVCPDSRFEAFKKNVDFIQKHIFPGSLLPSIAAINTAINETGDLFMFGLK
                     DMGKSYVITLSEWRNRFNLNVEKVRQLGFDDEFIRKWNYYFSYCEAAFDMRNINVVQM
                     VYTRPNNYNY"
     gene            complement(29509..>30220)
                     /locus_tag="PLB46_00140"
     CDS             complement(29509..>30220)
                     /locus_tag="PLB46_00140"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRF74946.1"
                     /translation="FMFWLDIDKLEQTTQSSKLFTYNKRGIYSFWDTDHFKYPTGDAR
                     NGLSTRQKLNDYLLSVGITKMPAQVFLTTHVRMLGYVFNPVSFYFCYDEQGTCNFVVT
                     EISNTFGEMKLFLVDAKNGEKFEQNAQKYFYVSPFTDMDTEFEFRYKVPDAKLNIQIN
                     VKEKSGDKFFISTLTGDKQVLKDARLLWYLLRFPFITIRVMTAIHWQALLLWLKKVPY
                     HKKSDDAQLQRDILNVKK"
BASE COUNT         9532 a         5734 c         5302 g         9652 t
ORIGIN      
        1 tattcgcata atctgttctc cagtctgtat gtccggccaa gcggtcaaat ttcttttcaa
       61 cgtcaccaat ataatcctca actgcttctt caactgttgc atagccactt aagccattgg
      121 caaaatcgta ttcaccggca ttaattgcag cctgtgtgaa ataatcgata tattcttcag
      181 catttaaaaa ctcgcgcaaa cgagttggtt cactatagcc ctgctgataa tctaaagtta
      241 tttttgcttt accttcttta ccgcgttttg tagtaataat taatacaccg ttagcagcac
      301 gtgaaccata aatggcactt gcagatgcat cttttaaaac ttctacactg gcaatatcat
      361 taaaatcaag atcatttaaa ggattgaggt aaacattaaa gtaatcgtta atagctactg
      421 tattaattgg aataccatct acaataatta aaggctggtt acttgcatta atagacgatg
      481 aacctctgat gcggattttt acatcgcctc ctactttacc gttattagat tcgataaaca
      541 aaccggctgc acgtccttgt aaggtttgtt caaccgtaga taccggtgtt agggcaattt
      601 catcggattt tatttctgag atacttccgg ttactttggt tttcacctca gttccgtagc
      661 caacaacaac tacttcttca agtatttctt gtgacatttt taagctcacg taaaccacat
      721 tcttctctcc gagcgtgatt ttttgggttt cgtaaccaac aaatgaaaaa acaagcgtac
      781 cgccactttc agggacattg atattatatt taccatcaaa gtcactaatt gtgccgtttt
      841 gggttccctc gagtaaaatg gttacacctg gcatgtattc gccattgtct gcatctgtaa
      901 ttttacctgt tatagtcctt ccctgcccga aaagctgcat ggagacgaag gaaatcataa
      961 tcaggagtag atatttccgc atagttttag gtttagtagg tcgctaaagt aaagcatttc
     1021 agcataacat tgaaaacaat ttcgtaaaaa aaatatgctt tttgtgacat ggttggcaat
     1081 tgttctgtca ccaaatctgt atatttcgac catgaaaaag caaaagttca ttatcagtat
     1141 gctgttttta gctgtttcgg cagtaacagc attggctcaa acccaggtac cagtagcacc
     1201 tgtggctgaa aaaattccct atgttattca aggttcacaa ggaccccgtc aagacccgta
     1261 ttattggatg aaggaccgtg aaaaccctaa ggtgatggaa caccttaaag cagaaaatgc
     1321 ttacctggag gcagtaatgg aaccaaccaa ggcacttcag gagcagatat ttaaagaatt
     1381 agtgggtcgt attgaggagg atgaaagcag cgtgccttat aagttgggag attattacta
     1441 ctattcgaga tacgaaaaag gtggcgagta tgccatttat tgcaggaaaa agggagactt
     1501 gtcagctaca gaagagatta ttgttaatgg gaacgattta gctgtaggaa atgagtattt
     1561 aagtttttat atagaaatga gccatgacca taaaatggca gctgtaataa tggatgttgc
     1621 gggcagaaat ttttatacga ttaaattaaa agacctcagc actggtgaat ggttaaaaga
     1681 tgaaattaaa gataccagaa atggatgtat ttggacgact gataataaat cgctggttta
     1741 tggtgtaccg gataaaaaaa cattacgtgt gtatcaaata aaaaaacatg tgttaggtga
     1801 taaatcaaaa aatgatgtag tgttgttaca agaggatgat caaacattag atttatatgc
     1861 gtatgaaagt agaagtaaag aatacatttt tgcttttgcc agcagaacag atgccggaat
     1921 aattaaatat acaaaagcaa atcaaccgga tgtatttact actattgagc ccatgacaac
     1981 tgaccattat tatacagttg atcacattaa aggtgatgaa tttgtagtaa gaacaaatta
     2041 tcaggcaaaa aattaccgct tgtgtaaaac aaaaattgca gcaccaacta aagaaaactg
     2101 ggcggatatt attccagcac gagataatgt atttttagaa acggtagaat tttttaaaga
     2161 ttattacatt gcacaggaaa gcaccaatgg attaactaca ttacgcgtaa ttgctccgag
     2221 tggtcaaaac gattatatca aatttgacga acctgcttat tatgccacac taggttataa
     2281 tcctgacccc aataccaatg atgtgcgatt tgtgtattct tcactaatta caccatattc
     2341 aacttatgaa tataatgtga caactaaagc caaaaaagta ttaaaacaag atattattcc
     2401 gagtggatat gataaatcgc aatatgtaac agaacgtatc atggtaactg cacgtgatgg
     2461 gaaaaaagtg ccaatgacta ttgcatataa aaaagataca tttaaaaaag atggcagtgc
     2521 accaggttat atttatggtt acggcgctta tggatatagc aatgaagatt atttcgacag
     2581 taatattttg agtttaatgg accgaggatt tgtgtatgcc aatacacata ttagaggtgg
     2641 acaggaaatg ggtggttcat ggtatgatga tggaaaaatg atgaacaaaa aaaattcgtt
     2701 ttatgatttt atcgattgca gcaaatattt aatacaggaa ggttatgtag ggaaacaaaa
     2761 actatttgcc aatggtggca gtgcaggtgg tttattaatg ggtgctgttt caaacatggc
     2821 accggaatta tatttaggta taattgctga tgttccgttt gttgatgtaa ttaccaccat
     2881 ggaagatgaa actattcctt taactacatt tgaatggtta gaatggggta atccaaaaaa
     2941 taaagcagaa tatgattata tgatgagtta ttcaccttat gataatgttg aagcaaaaaa
     3001 ttatcctaac atgttggtta caactggatt taatgatagt caggtacaat attttgaacc
     3061 agcaaaatgg gtagctaaat tacgtgcaac aaaaacggat aataatttat tattatttaa
     3121 aatcaattta gatgcgggac atggtgggca atcaggccgt tatgagagtt taaacgaaac
     3181 agcgtttatg tatacattta tgttacattg tttaggtgta acaaaataaa taatttacta
     3241 ccaataaata aaggccgcac tcataacgaa tgcggcctta tcttatttac taccgataaa
     3301 taaaacctta gtgttgaact acaaattttg cagtttgaat cccgtcagcg gtttggaaaa
     3361 ttgctacata cattccggaa gcgaatggca gcacatcaaa cgttaacgta ttttcgccgg
     3421 cggtgacagt tgttgctgtt aaccagttgg caattatttt acctgaaagg tctgttacaa
     3481 ataaattacc tgttgcttct ttttcaagat taaaatcaat ggaaactgat gttgaagcag
     3541 gtgaaggata tatgtcaaaa gttgttggct tattggcact taattcttcg tcttcaacat
     3601 tatccgcagc aaaacgtaaa tcatcaggat tgtaatctga aatttcacat tcatcgatcc
     3661 aggctgtcaa cgtacaacct gataatgctt caaaaccagg atgtaacgtg atataatttg
     3721 ccgattcaaa tgtaaccaca gcaggagaca aaattgttga actgcattca actaaaaatt
     3781 cacctaaaag cattgattta ttttcaatac cgtaagtagc atcgctgtaa ccggtcagta
     3841 tataagtacc acaaattgat gctaaatcat agctggtaaa tttagtaaca ccaaccgctt
     3901 gaaaggcttg acctacagct attacttcat cagagcaact gccataataa tctattgcag
     3961 attgaatcca tgcattacgt gcgataatat atccatctga accatcgaga taattcatca
     4021 tagcacccca tgcaattcgg cgagctttgt cataaccaat agcatcgacg tgatactcaa
     4081 ggccataatc atttacacca tctccacctt cactcaacaa ataaaatata tgattttgta
     4141 cgccactatt agtatgcact ccaccattat cgcctccgcc taaatctgcc caataatcac
     4201 caaaataagt atccgggtca cctttatcat taggattaat gaaggacctg ttggcacctc
     4261 tgtcttcacc atgcagccat gttggtgagc catcgccttc cacataaaat tctaccatct
     4321 caccaaaaat atcggcaaat gattcattta gtgcaccact ttcttctgaa taatctaaag
     4381 catcaaagtg tgcatattcg attacgccgt gtgtaaattc gtgaccaata atatcaattg
     4441 cattgtagtc atctgttgtt cctgctacac aatcattacc ggaaccaaaa tttagattat
     4501 caataatgtt ggtataattt gcgttggtgc aattcggatt atcatcatca tcaaaatatg
     4561 ttttattatt aaaaatatcg atatcgccac cggaaccatc aaaactttcg tggccatagg
     4621 tatttaaata atattcaaat gcttgtcgga ctccccataa agaagtcatt accatggtat
     4681 aattaccaaa tccttcccat gtgttggtgc ttgaacaata gtaccaatct tcaccataag
     4741 aaggcgtacc ataatcaccg gaaaaataac ttcttatttc tgtatccggg ttgcagtcat
     4801 ctataggaaa ataggatgtt tctgtatcac cgtaaggacc acaatcttca gtacgataat
     4861 cggtataaac agtttttgta ccgttaaaag tggtaaccac tgaagtactg ttacaaaaat
     4921 gttcaaggct gcggtcgcga attatttcgc cggtatttgc atttacataa acatatttag
     4981 ctattgaagg cagcgttgca aatacataca ttctatacgc caacacataa ttatctgccg
     5041 cgtttttttc tttcggattt attttcacta ataataattc cggtgttgga tataaagtag
     5101 catttgggtc tttctttttt gctttaaatg ctgcttcttt cttttcatct tcccacatat
     5161 atttttcagc aggatacgca gcaagagcaa tctcgatagc ttgctgtgct gaaattgtcg
     5221 gtattacatt cacatttaat ccgggtataa aatcaccatt aattaaatga attttaccat
     5281 tcctttcatg tgatagttgt tcaattcctt caatacgaac accattataa tactgagcaa
     5341 atttatagtg tttattacct gcttcatcga tattggtttt cattaaacgc aattcgtcgc
     5401 gttctgttaa acctaataag tcgggcgctt ctgtaaatac tgaggctgca tttaatttag
     5461 catcagattt aaattctatc cagtcctgag tagcgccagg tttgataata gcctggtatt
     5521 ttttttcaac atgtccaggc aatacccgtt gttgcgcttc taatagttgc gtgcttacac
     5581 aaagtaaacc tattaaaagc agtttccatt ttttcataaa aattaattta atggtgaata
     5641 aaaaattatt ccgcttttaa tgcatcaatt tgtttttgca gttcgatgat atataaagtg
     5701 agttcttcaa ttttttccat catttgaact tgcattgtgc ctacttcaat accttctgct
     5761 tcaacaacat cagctgaagg aataccaggt aagtgtttat tttgtttaat acttgcttct
     5821 aattcatata aagaaggtaa tgcataatta tcggcaaata cataatcagg ccagctgcca
     5881 ctcatttgca cttttaattc ttcacacatt actttaccat ctacagataa taaataaccg
     5941 gttgcaggaa ctgaagttcc aatcataaca ctaccaccaa catcacacat gctcacatta
     6001 ccaccatcat tatttaaata taatgttgat gctgcgccat tgttacgtgc cataatttca
     6061 ttattatcaa tggcaatatt taaacttgtg gcgtcaccgc tgacaataaa acctccggaa
     6121 gttagttctg catctgtgcc acccattacc tgaactttat aagcaggagt tgcagcgcct
     6181 acacctaagt tacctgaaaa atatccacga cctacgaaat aaccggccca ttgtgaagtt
     6241 ccgctagttg cagttccata aacaccaatt gataatcctg cagttgaacc tgctgttcca
     6301 taaacaccat aagttgtgcc cgcagcacca aatcctgaac cgtaaacacc ataacctgtt
     6361 tcagctccgt ttgatgcaac acctttaaca ccaatttgac caccagatgc ttgcaaaccg
     6421 gtaccatatc caaaagtata agtattgctt atttcaacaa gattaaaatt ggttgcacca
     6481 acaccagtcc atggtttagt tactttcata actgcagttg ttcctgttgt ttcaacattt
     6541 aattttgcac tcggtgtaat tgttcctata ccgatttgac cggtattatc tactacaaac
     6601 tgatcacctc cgttgtataa tctaaattga cttgtagctt catcgtagta agtccatgct
     6661 tttgctgaac cacctactgc ataaccataa aatggtttac gtccgcttgt tccggccata
     6721 tcaatataca taccgccata agatgttaca ttgtttgatt ttgccacaaa gtttgctgaa
     6781 ccaatcattg ttgtggtatt gatacctaca ttaccgccgt tatcatagat tgatgagttt
     6841 ccaacggtag ttgcagcagt gaatttcgga atgtaattgg ctgttccgct tcctcctact
     6901 ccaccgcctc cgccaccaac agttgcagtt gattgtgttg ttccatccat aaatttgatg
     6961 gagttcacat atacttcatt ccaattattg gttgacgaac ctaaatcgag tgtgttgtta
     7021 gtagctggtg ttaaacctac attaattgat gttccagctg cagctaaatt ggataaacta
     7081 gtgttggcac ctttcgacga catggctgcc caagaactac cggtgtaata ataaaatccc
     7141 ggtgtggaat tagtttggaa aattaataat ccggtagcag gagcaacaat tgcatcgcgt
     7201 tgtgcttttg tcatgcgagg tgctaataat ccttgtgaag tactggtcat gtccaataat
     7261 gccgaagctg ttggtgttgt tgtgccaagt cctgcactac cggttaacgg atacgtgttg
     7321 gtttgcgctt taaccattgt tgcacctgca attaatgctg ctgcaaataa aagtaattgc
     7381 tttttcattt tgatttgttt taaaatttta aaaatgggat gaataaatag ggtttgtttg
     7441 attaaccacc aaacatgaag cagactttac aatttgcaat gcgaggataa catattgcga
     7501 gatgtgaaaa atttgtttca taacggtagt attagttgca aagagaaggc atacggatag
     7561 ggcaaaccag tagatttttt tgactttgta gcagacaatt tgcaattttg agcatggaaa
     7621 atacctattt gcagcaggtg ttacaggtac tcagcaaagc agaatggcga gatttcgggg
     7681 attttgtagc ctcgccttat ttcaataaaa atgagaagtt tgtaagattc tatcaatttc
     7741 ttcaaccatt ttatccaaaa tttgatatta caggtagttc aaaggaacaa ctgtatatac
     7801 atgcaacttc tgccgacaag tatagtgatg caggttatcg caacttatgc agcgatttat
     7861 tggtattagt acaggaattc ctggcacaag agtcgttaaa tgcaggtact accataaagg
     7921 atagtttaat taaccgaaca ttaatattaa gaggtgtatt agaccttgca gaaaggaatg
     7981 taaaaaaagc cgaaacaatg gttgccaaaa gagattacga ttttcaggaa caactcatgg
     8041 ctgccatatg gataaatgat ttgcggatac tgcataatat ttacaacaac agaaaaagta
     8101 tgaatgcctc caatcccatt atgctggagg acaatacgca taaatttatt actgaatggg
     8161 cgttgttgcg tattttttca tcattaatga ataatattaa agctacgaga agttataatt
     8221 taccattaaa tactcagaaa tatcaggcat accttaattt atatgaatcg ttcgagccgt
     8281 ttaataataa agagtcgcgc atcatgtatt taaccgttaa aatgaatgtg ttaaatgatg
     8341 aacaagctta tttcgaatgt tttgaaattt taaaaaccct cgattttacg aaaaatatca
     8401 ggacaaatga aaatttagta attggcctat tagactttgc attacataaa ttaaacgaag
     8461 atgaaaaatg gcggattgaa ctacatgaat tatatgattt gaagctttca aataaattgt
     8521 ggagtgaaaa aaagatgtta tcttatacat ctttatttaa tgccgtgcat aacagtttac
     8581 agctaaataa gataagctat gcagaaacta ttttagatac ttatatacaa gatgtttcgc
     8641 caacaattgc cacaactata gataatttat gcagagcttg gattttcttt tttaagggcg
     8701 attatgataa agcacatgag taccttgtaa aagtagaaac agaaaatatt cttgtaaaat
     8761 ttgaaatgag aagtttgcag tgtctcatat acctacaaaa gaaagaatgg gaattattaa
     8821 atgcagcctt agaaagtttt agacaattta acgcttacaa caaaagcagc atcaacgaat
     8881 tggtgacact ccaatttact atattttgta aatatatcag ctcaatagcg aaactacatc
     8941 ccgattatct ggaaaaggat aaaataaaaa ttcaggacca gataaagtca gaaaaaaatt
     9001 cttacatgcg caattggatg ttagaaaaaa ttaattcagg aaaataatta ttgtttaatg
     9061 gggaattctt caccgggtgc ataaatatta aggtgaattt gtcttgccgt caacaaagta
     9121 tctctgctca tatccacttt aattggggct cctacttcaa caacttgact aacgccaaca
     9181 acttttgcca ttccctggct gactaaaatg gcagttcctt catctatacc gatacattga
     9241 aaccctttgt tctcaattgt tgcgcataac aaacgattat atctgcttcg ggctacaaaa
     9301 tgttggtcga taattaccga atctaataat cctaaacctt caactaattc aagattgcct
     9361 ttccagatat tatcgaatgt tgattcatac atcgtatcaa ctagaccctc accggttatc
     9421 atacgttcac tcataatact tgctccagca cttgttccgc tgattaaagc accattttga
     9481 taagcttcat taatagcagc cttaatcgtt gtacctttaa ttaaccgcat catcacagcc
     9541 tgatctccac cactaataaa aattattttt gattttctta aagagtcaat tttttcaggc
     9601 acatttacag ctgcagaatc aaacttaaca caattttgtc cggttaactt tttgaaatcg
     9661 ttattcaacc aaatataggc gctatcgccc caaccacttg gcaaggtaat agtagtaatt
     9721 aactcacccg gcttccatcc tgaagctaca attaaatcca tcattaaact atcacctctg
     9781 tcgccaccgc caataataaa caatttccct ttagggtaat cggttttggt attcgatgtt
     9841 tcttttgatt cagggctggt acaggcaaca aataacaaag cgcatactgc agctacaagt
     9901 gatattcttt tcatgtagtg gtaattttga acaaatataa ggatattcca taatcggtaa
     9961 attatttacc aatggagaag ataaaagtag tgcctttgcc cggtttcgac tcaacggcta
    10021 tttctcccct atgccgttcc acaatttttt tgcaaataga caatcctaaa ccggagcctt
    10081 gtatttcgtc gcggccgtgt aagcgattaa aaataccgaa tattttttgc tgaaactgtt
    10141 gagctatacc aatgccatta tcttttactt taaattccca gcggtcagtt aattcctcca
    10201 aatcgataaa caccagaggt tcgtttttac tgcggaattt aatagcatta tcaatcaggt
    10261 tctggaacaa taaaatgatt tgcgaatgat tgccatatat caccggtaaa tcgggatata
    10321 aaatcagtgc attactttct tctaccgaca tttgtaaatt atctaacgcc tgacgaatag
    10381 cagcattacc ttcaaagtgt gtcattggtt cactttgaaa gtccagttta ctataggtaa
    10441 gtaaatcttt aattaaatga tgcatacgtt gagcaccatc ggaagcaaat tgcaaatatt
    10501 ctttgccttc ttcagtaagt gttgcaccat aacttttcag aaataaatta atgtaagaag
    10561 caatggttcg caaaggctcc tgcaaatcgt gtgatgcaga atatgcaaac tgctgtaatt
    10621 cctgattgta ggtaattaat ttttcgttga gctgaaacag tgtatcatta ttttccgcaa
    10681 tatggccgga aagttctttt tgtttgtcgt taattttact tctgattaac agtgcatcct
    10741 caacctgcca aaagaaattt gaaaaaatac ggcaagtatc attttgaaat gcatcttctt
    10801 caataggagt aaatgaagtg ccataaaaaa ttaattcaat attacctgca cggcgaatgg
    10861 ttacaataac ttctgctgca agttgttcaa ttttttttcc aaataaaaaa ggtcgatcat
    10921 cctggttgat aaaaacgtat ttatctttag catctaaaat tgaaacaggt aattgaattc
    10981 tattttccgg ttttgcaatc ggtactgatt tcttttttat tttacattct gccgaacgaa
    11041 taaacacatc ttttttttcg cttacgcgat acaatgaaca ataatctaag tggaacaaca
    11101 aattaaattg ttctgcaaaa tacaacatag cctcatcggt agttcgaagc gaagtaattc
    11161 gttcggacat acctgctaca atgctgatat aagctgcgtt ggtcaatttt taggcttaat
    11221 gtgtttgcaa gattaggcgt tcaaaacgaa acaaacaaat ttaatttttt atcaataaac
    11281 cctaatacag gaatacgaag tccttagccg cttttctctc gatatcgggc actgttgcat
    11341 cagcagccgg ataacctata ggaattagta aaaagggcgt ctcattttca ggcctttgca
    11401 acaaattttt aagaaatccc attgggcttg gcgtatgggt taatgcgcat aaaccggcat
    11461 tttggatggc tgccaatagc attccgacag ctattccaac actttcggtg acataataat
    11521 tatttttctt tctgccatcc actacctcat aatttcgttt aaaaacgaca attaaggccg
    11581 gggaggtttc taaaaaaggt ttatgccaat cggtgcctaa aggcgccaaa tctgccagcc
    11641 attcttctgg cattctttca ctgtaactca gttgttcttc cttttcagca gccatacgaa
    11701 tttcgtgttt caacttcgga ttagttacca cacatattga ccatggctgt ttgttagccc
    11761 ctgaaggtgc cgtacctgca gtcataataa tattcgacaa aacaatttcc ggtataggtt
    11821 cgtcactaaa aaaacgcagg gaacgacgtt tttgcattaa ctcataaaac tgattacttc
    11881 gaattaccat ttcatgttcc gaaaaacgca ccgggttaaa tggaataaaa ggatactcat
    11941 tcataattct gtcattttat ttgtaaaaaa tgttgcgcat cttcgccctg attttacgtg
    12001 aaggtaataa ctgcatatca aactattcaa tcaccatcgg aaggtattta taccgaagcc
    12061 ggcagtcgtt ttttaggata cttatatcca cttaccaccg atgaagaagt aaaacaaata
    12121 agggataact taaaaaagga acatcacaaa gccgttcatg ttgcctgggc ttgtcgctta
    12181 ggcattttac cagatacaga gcggagttcc gatgatggtg aaccctcagg aagtgccgga
    12241 aaacccattt taaatgagtt aaaaagtaac aaccttacac aagttggatt atttgttgtg
    12301 agatattacg gcggtaaaaa attaggaatt cccggtttaa ttcatgctta tggagcagct
    12361 gcagcttata gcataacatc agcaaaagtg gagactattc caatcaaaga atgttacaag
    12421 atttcatgta aagaaactga tttacacctt gtaattcacg aggtaaataa aattggcgct
    12481 aaaatcgttt cgacagagtt cggagaaata tgtaatttta ttataacatt taatcgcgac
    12541 aaacatgata aagttattgg caaattggaa acgctttggc aaattgaatt ggcatatagt
    12601 catagctttt agtttttgta gctgcttcac tcaaacaatt ttcgctcaaa atgttaccac
    12661 gcctgatatc cagctcgctg aaaattttat cgcctcccgc ttaaattacg atgagttata
    12721 cttaagtact tttgtttgtg ttgccaccag acaatcccct tattccactt attatacatt
    12781 tcaacaatat tttaaccata tcccgattga aaataaactg ttaaaagttg gtatcgacga
    12841 taaacatcaa ctaacgtttt ttgcaggtga ttatcaaaca acaaccggtt ggacaaatcg
    12901 agccatggtt gctaaaaaat taaatactgc ttatttatta cccaatggta aattaattaa
    12961 tgctaatact ccactgcaac cactgattcg tgttgtggaa aaccaaccaa tatatgtgta
    13021 cacatacgat ttacagaacg aagaccgttc ccgatacaca atagtggtta atgaaaacga
    13081 tgaagtaatt gaagtacagg atcataaagt attttttgca ggtgaagatt caactgtttt
    13141 ggcaaaagta tttttgcccg acccacttac atcggcagaa gtaacttatg gcgcgccatt
    13201 tgtggatgat aacgatagtg atattgccga attaaataat gaagcagcaa ttgttaccat
    13261 gaaagtattg tttgaggacg gcgtatttta tcttaaaaat gataatatta taattaaaga
    13321 tattaatgtg cctactgcag ccgttgttac ttcaattatt cctgaattta attatactcg
    13381 aagtgaaagc ggatttgagg atgtgaatac gttttatcat ctgactaatt tttgcaatga
    13441 tattgtagca gccggatata cttccttgca ggatttttat attgaaatag acccacatgg
    13501 agcaagtggt gctgatcaat ccttttatgt aagtgcagct attcctacta ttcaatttgg
    13561 ggaaggtggt gttgacgatg cggaagatgc agatgtaatt gtgcatgagt ttagccatgc
    13621 attgagcgac catgcttctc ccctttctaa ttcgggatta gaacgtcgag ctattgatga
    13681 gggttatggc gattattttg ctgcaagcta ttcacgaaaa ttcagcgatt ataattggga
    13741 aaaaattttt tcctgggatg gacataatga gttttggttt ggcagaaatg cgaatacgac
    13801 taaacactat cctgaagata attccgataa tatttatgcc gccagtgaaa tttggtccgg
    13861 tgcattgatg gatatttttg atgtaattga aaaaaccaat tgtgaccagt tagtttacga
    13921 agctttattt ggaagtttcc ctaatatgac tatgcctgat ggggcatcat taattttaaa
    13981 tgctgaagca actatttttg gcggattata ttacgaacaa gtatatgatg cactttttgc
    14041 aagaggttta atttacccga atgcggtaac aaatatcacg aataataaaa ttcagatatt
    14101 aaataccaat ggttttgcct tttttgatga acctctatta attcagttca atcaatatac
    14161 agatgttcaa cttaactgtt atagtgtaga cggcaagtta ttagcgcatc agcaaacatc
    14221 tggtgcaact ataagctgga aaccggaaat aactcagcag ggattattta ttatagaagt
    14281 ggtaacacca acaggcacaa tagcaactaa aatcagtcac tattaattat tcgctgtctt
    14341 catcttcagg gcggaaactt aacacaggta tggtgctatg tgaaatcact ttattaatgc
    14401 gcgaaccaaa aatgagctga ttaaatttat tttctgcgtg gctcataatt acaagtaagt
    14461 cggcatcaat ttcatccgca tataaaagaa tagaattgct caccatgtcg tgacgaatca
    14521 tttcggttgt gcatttgaga ccatgattgc gaattgtatc agcagcctct tccatttttt
    14581 cttctaaagc tgttttactg gaaataaatt cttccaaaaa ctcagaaact gcaaccaggt
    14641 gaatagtagc atcaaatcct ttggcaaaac ggataattcg ttccaccttt tcactagtat
    14701 attttgttaa atcgataggc aacaggaaat tcttaaactg gataggcgac ttaacatctc
    14761 tgcaggtaag cacgggaaaa tcgacgcttt ttaccagttt ttcggtgtta ttacctaagt
    14821 taaacttatt cggtttggaa tcggacccga aagtgccaat acaggccagt tcagcaccga
    14881 tttttaagcc ataatcgtga attaagcccg gaatagggtc attggtggtt ataacttcgg
    14941 tagtaattct aaacgcagct ccactggtat tcttcatttc gtcgagcctg gcctctaaag
    15001 cgcggatttt ggccttataa gcagaagcgg tatcgttttt ttcctttaaa gattcacgaa
    15061 gggcaaaatg aaccagatgg atatgcgagt ttgttctgtt tccaatatat attgcatatt
    15121 gtaaagccgc cactgaagcc gaggagaaat cagttgggac aaatatttgc tttagctgct
    15181 cactcattcc gagcgcaaaa ataatcagaa tttttacaat taactttgta ggtatatgtc
    15241 aacaacttca tcatcggagc ttattttaaa cagcgatggc agtgtttatc atctgcattt
    15301 attacctgaa caagttgcag atactgttat aacggtaggt gatccggaca gggttgctat
    15361 ggtgagcaaa catttcgaca ggattgaaca tcgagtacaa aaacgcgaat ttattaccca
    15421 taccggttat atcggacata aacgaataac ggttatttca acaggaattg gtcctgataa
    15481 tattgatatt gtttttaatg aattaaatat tttacgcaat tataatttta acacccaatc
    15541 atttaatgcg atagctaatc ccttacaaat aattcgtttt ggaacatcgg gagctattca
    15601 ggcagatatt ccggtgaata gtttattggc ctccggtttt ggcattggga tagataatgt
    15661 gttgcacttt tatcaatttg aaaacaccaa agaagaagca gctatttgcg aacaatttaa
    15721 attgcattgt tatttcaatc acaccataca tccttatgta tttagcgcag atacacattt
    15781 gttagcgcaa ttagataata catttataaa aggcatcaca ttaactgctc cggggtttta
    15841 tgcgccgcaa ggacgtgctg taggatatgc attaacacat ccggaatttt ataacgattt
    15901 aaggagtttt cgtcatttgg actggcggat taccaatata gaaatggaaa cagccaccat
    15961 gtttggttta tcgcgagtgt tgcaacatca atgtttgtcg ttaaatgtat tgctcgccaa
    16021 cagagcgaca ggcactttta gcacagaccc tactgctgca attgaaaatc taattcacca
    16081 tggacttacc tttatagcag gattgtaaat tattctactt cgatattagt attagctctt
    16141 actttgctga tgaataaatc caatgctctt cgttttgtag tatcgagatt ataattaata
    16201 tcgttttgca aataatcttg cacttcagca atggtaaaat tgggatgcgt ataatttttt
    16261 gcaactaaat caatatgttg cacaccaaat ttaagtgctt catttatttc ggaaataata
    16321 tcttccggaa ttgttttacg ggtaatccat gctgcaaata caaaaggtaa acccgtcatt
    16381 tttttccagg catcacttaa atcataaaca taagggaatt ttttattgag ggctaatgct
    16441 ctgtctccaa ttactaatcc cgctgttgta ccttttattt gatcaatata accggtttca
    16501 gctgcaattt gtataggatt tacctgccag tattcacgca acagtatttt aaccagttgc
    16561 actgaagtaa gggaatgata atctaaaaat accttttcaa ttttatcaat tggttgttga
    16621 gaaaagatac ttacactttt tacaggtcca tcggcaccaa tacaataatc agttatgatg
    16681 cgggcatcgt ttaacttagc taaaacagct acaggaacca gaccgatatc aacgctgccg
    16741 ctgagtattt ttttcccgcc aacagatgga atatctagtt gtaaatcgag tttttctttt
    16801 atcgcagcat tttccagccc atataaaaat ggtttggtat tgagataact cactaacgaa
    16861 atgatatatt tattatccat ttaaatgtcc tatatcatta aacaaaacta ttttaaaacc
    16921 gttcgcatta tagtgcaatt tatacaaact tacattatga tgcggaaacg tgtccatgtc
    16981 tttaagtggt tgattgagca tagtgcataa taaaatgcga atagctctgc cgtgactaca
    17041 aatcaggatt ttcttttctt tggtggcctc taacattttt acaaattttt gctgccgaaa
    17101 ttgcacttcc tcagggcttt caccttcatc aatttttacg gtagtttcac ctgcctgcca
    17161 taaatgtaaa atgcgataaa agtctttgaa agtatcgcca tcttttttcc cttcgtgaat
    17221 cccccaactt atttcatcaa gtccttcatg tatttcaaca ggaatattta attctctgaa
    17281 gggtgcaact gtttgcgcag ttctttgtaa ttgggaaata taaatttttt caaaattttc
    17341 gttacgataa gcttcaaaaa atgcctgagc ttgttgtgtt ccattagcat tgatagagct
    17401 gttcacacct tttccctgaa caattcccaa ctggttgaaa tcggtttccc catgtcggat
    17461 aataaatatt tccttttcca aatcagttat taattatagg caaattcaca aaatgatcga
    17521 caccaaattc agcatctaaa tcaacattag cataattttt tatggtatga taaactgtat
    17581 ctctttccac aggaattttt ccagctgtta gaatcagttc acaaatttct tttgtggtca
    17641 ttaccggtga ttgctcttcg gcgccggcca ttgaataaat ttttgtggta tcgtcaatag
    17701 taccatctat atcatttaca ccgaaattga gacttaactg tgcattttgg cgaccaatca
    17761 ttggccaata tgctttgata tgtgggaaat tatctaaata aattctggca atagcataat
    17821 tgcgcaaatc ctcaatcaca ctcacttcct gcacatgttc catctgatta tttttatttc
    17881 tgaattttag tggaataaaa caattaaatc cacctgtttc atcttgtaat ttacgcaagc
    17941 gctccatatg gtccactctg ttggtataac tttcaacatg tccatataaa attgttgcat
    18001 tactaggcaa tcctaattca tgtgcagttt tgtgtatttc gagccatgtt gctgaatcgc
    18061 atttatcagc acaaatttgt gagcgaacag tttcgtcaaa tatttctgca ccgcctccgg
    18121 gtaaagaacc taatccggca tcttttaaaa attgtaatcc ttctcgaatc gacatttttg
    18181 ctttgcgaat catgtattcc aattcaacag cagtaaatcc tttcacatgc aaatcaggac
    18241 gcagaatttt aatatcgcgg attatatcag caaaccatct caaatttaat tccggtaaaa
    18301 caccaccaac aacatgcact tcagtaaccg gtttgttttg gtaagaacga acaatatcca
    18361 acatttcatc actcgacatc acccaacctt cacttctttg tttaagcaaa cgggaatagg
    18421 aacaaaattt acaatcgaat acacaaagat tggttggttc gatatggaaa ttacggttaa
    18481 agtacgtaac acttccatgt tttttggtgc gaatataatc tgccaatgtt cctacaaaac
    18541 tcaaacttgc ctgttcataa agcgttacac cttcatcaaa agaaatgcgt tgtccggcaa
    18601 gtactttttt tgcaattgag cgcaattgct cattggttgt agtagtttca attaattgct
    18661 gaatatcatc ttcacgtgtc atcttcgaaa atttatataa aaaaagagtg gttaggtttt
    18721 attcccaacc actctataat tatttattga taattaagca tactattatt taataacagt
    18781 aattttttga gtcaggtaaa actcaccatc tgaagcttta accatataat cgcctgcgct
    18841 taaattgctg gcatcaaata caacatgttc ttcaccttta ttcgtggtaa ttatctggtc
    18901 gattaactga cctgttaagt tgtatacttc aatagtaagt tcattatgaa cgaaattctt
    18961 catttcaaaa acaaattcac cactagaagg gttcggataa atacttaatt gtattaattg
    19021 attttttaaa acttcattta catctacacc accttgcaca caaccttgag aagtcaataa
    19081 agattcacgg tcattttcta ttacatcgcg catttcagca gcctggtctt ctgaaaacat
    19141 attcatagca ccatcgtcca catagtccat atagttcata aacatgatac cagggcttga
    19201 aggagaacaa tcgtccaaac gagggaaaga aggattgcca taagtagcat cttcctgatt
    19261 aggtgtatct gaaatgccgt cagaaccacc acattgcggt tcagcagcaa aatcgtcatc
    19321 accccaagta tgatttaagc ctaaccagtg tccaacttcg tgtgttgctg tgcggccttt
    19381 attataaacg ccacctggac tgccatcacc aaaatatttg taaccgatta ctacaccatc
    19441 ttttccggaa ggaccacctg gaggagttgc ataacctaaa acaccaccgg taaggttaca
    19501 tacccaaata tttaaataat cagttttagg ccaagcgtct tcaccgcctt tatctgtaaa
    19561 tttaacgtta tctgaatagt tatctgcacc aacaccaaca aataaactcc agctgctaat
    19621 atcagtttct gttctggtga ttccactatg tgcaacacca tcagggtctt gttgagctaa
    19681 acagaactca atttgtgcat cagctgctaa tccttcgaat gctgcaggag tttcatcagc
    19741 atcatcgtta agtcttctat agtcagcatt taaaacatca atttgagata aaatgaattc
    19801 atcgtcaata ttttgcgaag cagcgctata caccacatgc accacaacag gaatggtata
    19861 aacgccacca ttggttttgt aggtagtaga caccttgttt gcatctcgtt ttattgcagc
    19921 ttccttaatt gcagcaaatt cttcaggaaa attagtttct aattgtttaa ggtaagtatt
    19981 tgagccacaa cgaactacag tttgtccaaa actaaccgtt gataaagcca gcaataaacc
    20041 ggcaaataaa gcttttttca ttattgatta ggattaaatt tacagattgt caacaaagtt
    20101 aaacagaacc cctaaaattt taccatatct gtaaaagttt atttgaaaat tggccgctta
    20161 ttgacaattt gcaaacaatt gaactttatg gtttaatata ccataaattg ttgggtcaag
    20221 aagtaggttc cattaaacac ctgaactatg taacaacctg aagctaaata acttaaatcg
    20281 aaatattgag ccacattgtt ggctgcagag gggtttgcag aggcaattat ttggccaagg
    20341 ctattataaa cagcgatatt aaaattatcc tgataaccta aaaagttctt gattgatacg
    20401 gtaaactgac cggaggtagg gacaggatag acaagaattt cggctatgtc accggggagc
    20461 gggccaattt cgttgcaacc tgctgcagaa gtgagcaaag acgctctata tgtttccatt
    20521 actgagcgca ttctggtttt ttgatcttcg gtaaacaggt tcatgcaagc gtcatctgtg
    20581 taatccatat aattttgata tatgatgcca ggcgccgttg gtgaacatgc atctactaaa
    20641 actgtatttg gacaaccata agttgcttca gcctgatcgg gagtgtcgtt gataccatct
    20701 gaacctgcgc aggttccgcc atcatctccc caaacatggg ttaatcctaa ccagtgtcca
    20761 acctcatgag tacaggtgcg acctaaatca tatggtttag aactaacatc agctatacca
    20821 aagtttttat aatctataac aacgccatct acttcggggc ttgcaccagg tgaaacagcg
    20881 taacctagaa tgccatcctg caaatcgcaa acccaaatat tcagataagc ggatgaaggc
    20941 caggcatcat cacccatttg gctcgtttct tttacttctt ctgaaaaact caacaaccat
    21001 tccgtagtat ccgtatatgt tctgataatt ccggtagtag aatctccatt agggtcataa
    21061 aatgcaaggc aaaattcaat acctacatct gcagctactg attcaaatcc tgggagtgtc
    21121 tttattgtat cggcatttaa gcgacgaaaa tcctcattta aaacatttat ctgagtgtaa
    21181 acacgagtat catcaatatt ttccgcttca gtgttgtata caatatgcac tacaacagga
    21241 attacataaa tgtcatcagt aattttggca ttaacctgtg ttttacttcg ctcttttatt
    21301 ttagcatata tttccggatt agtagtttgc atttgttcca gaatagctga tgttccgcat
    21361 ttttcctgcg caaataataa attgctgcta caacaaacga gtattaatat gaggtaaatt
    21421 cctgctcgca taaaatcgaa agatactaat ttttacggtt ttatatgttt cgaataagcc
    21481 cgccattttt cagttgctgc taaaaaatca tcgggtaatt cggtttcaaa atacatttct
    21541 tttcctgttt tagggtgaat aaatcctaaa gaacgagcgt gtaatgcctg acgcggtatt
    21601 aacgcaaaac aattgtctac aaactgtcgg tatttggtat aaatagtacc tctaacgatt
    21661 ttatcaccac cataagtatt atcgctaaac acagggtggc caatatgttg catgtgcact
    21721 cttatttggt gcgttctacc tgtttcaagt ttacattcaa ttaaactggt atgaccaaaa
    21781 ctttccagta ctttataatg cgtaaccgca tgtttgccaa tttcccctcc gggaggatat
    21841 acagtaaata attttctata tcgttgatgt ctggctatat tgccaaccac tgttccttct
    21901 tgttcatcaa attcacccca cactaatgca ttataacgac ggttaatggt atggtcaaca
    21961 acttgcttgg ccaaatgcgc catcgcaaat tcattttttg cagcaataat taatcctgaa
    22021 gtaaatttat cgatacgatg cactaaaccc ggtcgataag gttcatcttg tttaggcggg
    22081 agttgttcga aatgatacaa caaggcattt actaaagttc ctgaaaaatt tcccagcccg
    22141 gggtgcacta ccatcccggg ttttttattt attaatacta catcatcatc ttcataaaca
    22201 atatcaatag gaatgttttc tgccctaaca tcatattttg ccggtttttg tggcaataca
    22261 acaactattt catcgaatgg ttttatttta taattcgatt taaccggctt ttggttgact
    22321 aaaatacatc ctgcttcagc ggcactttga atgcgcgttc ttgaaatgtt ttcaatacgg
    22381 ttaaataaaa atttatcgat gcgcaaaggt tcttgtttcc ggtctgtaac tacacgataa
    22441 tgttcgtata attcttcatc ggattcagct cctaaatcac caatttctag atcatcatca
    22501 aattcctgca tcagattaat ttacaattaa aaacggtagc aaaatgttgt ttaagttttg
    22561 ccttcacttc atcaagcggg atttgtttgc ccaattcttt ttctaatgaa gttacagcct
    22621 tatcctgaat accacaaggt ataattagtt taaaattatt taaatcggta tttacattaa
    22681 aggcaaatcc gtgcatggta acccagcgac tgcttctgat gccaatagca caaattttac
    22741 gtgcattttt attggttgca tctaaccaaa cgcctgttgc accgggtaaa cgttcacctt
    22801 gtaagccgta atcagcaagc gttaaaataa ttacctcttc aatacttcgt aaatatttac
    22861 ccaaatcggt aaaaaaataa tccaggtcca aaatggggta tccaacaatt tggccggggc
    22921 cgtgataagt aacatcacct cctctattaa tatgaaataa acttacgccg tgttgattta
    22981 acaatgtttc gtttaataac acattatttt cattggcact ttttcctaat gttattaccg
    23041 ggtcgtgttc aacaaataat aattgatggg aataagttaa ttgttcttca tcattcagta
    23101 ctctgttttt gagttttttt tgtacgatat cattgaaaat actttcctgc aatttccatg
    23161 catagccgta ttccattttt cctaaatcgc gaaagtgaac gagttgcatt attttttttc
    23221 caatgctgtt tttaaccaat cgagcatgtc gattttaata gggtcaacac ctttgagaat
    23281 tgataagtaa agactcatcc aatcgcccag atgtattagg taaaacatat tttcaattgt
    23341 gtgattacct gctgaaccaa caaccacagt acaacccact ttatcactaa ttaattcatt
    23401 taccaattca aatcggcgtg ttgtttgcgg gtgttcatta ccatggcgca gaaatactgc
    23461 tgctatagct gaatttttgg tgtgaaaagc aaccagttcg ttgtggttca tttcaggaat
    23521 cacatgacaa aaacaatgca tttttgcatt ttcattaatt tgttgcttcc atcttaatac
    23581 aacagcctgt aaagtcgcat caccataaat tataggaatt gtttgttgca actgatcggc
    23641 caatgttttt gcagtcaatt ggatatcatc gcgtttattg attaataaat gtgcagcttc
    23701 ttttatttct tcaataaaat aatcgttgat gattccggca cattttaata caaataattg
    23761 tgctacggta gaatatccta aacatgaacg tggtggcata cctcccggga taataatatg
    23821 cggtaagtta tttttaatgg ccttttctaa taacttcccc cctgttgtaa cgcaggcaat
    23881 catacatttt tttcgcagcg cctggtcaaa ggtggcaagg gtttcttctg tatttcctga
    23941 ataactggaa caaatcacca atgtattttc gccacaatgc aagggcaatg tgtaatcatt
    24001 tattatttca atcggtgtct taattcgctg tttgaggatg tcctttatca gcattccgcc
    24061 aattccggag cctcccatac cggaaattac aatatgatgc aatgggtttt ggagcttaaa
    24121 atcagatgca gcaagcccga tttccacggc ttccagtagt tgttgaggaa atctatcaat
    24181 atatttgtcc atgttctgaa acacaaaaat acattattat gggcaggcat aacgaattgg
    24241 ggaggcttgg agaggagaaa gctgccgaat ttctcgaaaa taaagggtat acaattttgg
    24301 ccaaaaatta cagatttgga cgagctgagg tggatattat cgcatccaac agccaacatg
    24361 ttttatttgt agaagtaaaa acgcgaacaa actatctgtt tggatatccg gaagaagcag
    24421 taggtgccaa aaagcaaaga ttgatgagcc gggcggcatc tgaatttatg tttcagcatc
    24481 aactcaattt acctttgcgt ttcgatgtta ttgctatctt taaaactaaa ataaatgaat
    24541 ggcaaattca acatttggaa gatgcctttt ttaattacgg atgataacag gcgtaaatca
    24601 tattgcaatc atttgttcgg attatcacat atcgaaacac ttttatcatg agatattagg
    24661 ttttgaaatt attgctgaaa actacagagc agcgcgcaat tcctggaagt tggatttacg
    24721 agtaaatgaa tatacagcta tagaattatt ttcttttgaa gatgcacctc cacggctgtc
    24781 gtatccggaa gccagaggtt tgcggcatat ttgttttagc gtgaaagatt tagatgctgc
    24841 gaccaaaaaa ttaagtgctt tgggaattac tttcgaggcc atcagaatag atgaatatac
    24901 aggtaaacga tttgtatttt ttgcagaccc tgatgattta cctatcgaat tatatgagca
    24961 ataaaaaatc ctgattaaca aaggctaacc aggatttttc tatttactaa atagtagcaa
    25021 ttaatatctg tcaaatcctt tgttttccaa aaaatctttc atttgttttc tagcaaaaaa
    25081 tattctgctt ttaacggttc ctaaagggag gttcattacg tcggctattt cttcatattt
    25141 ataaccctgg taatgcatga taaatggttt tttgtaatct tcatctaact ggttaatagc
    25201 cttgatgagg tctttcatca caaagtcatt ctcagcctga ttggcaatag ccccaacact
    25261 ggaattcaat aaaaataaat tttctgaagt atctgaaaca gtgttgcgtt ttgacgcttt
    25321 gcggtaatta ttgatgaaaa tgtttttcat gatggtaagt aaccatgctt tcagattggt
    25381 accatcgttg aatttatctt gattggacaa agcacgcata tatgtctcct gcactaaatc
    25441 aagcgcatcg tctctgtcgc gagtcaggtt caacgcaaaa gccttaagtg gcgattttag
    25501 ttgaatgatg tgtgattcga attcagtgtt tgtcatattc agatttttat acaaagtaag
    25561 ccatgaaatg tcgtaattcc aaatttttgt ttaatctttt ttgttaaatg tttaacaaaa
    25621 ccatgaaatc aagctacagc aaggttttct aattgagcca ccactgtttc caaactttca
    25681 attacataca cgtttgccga taaaccctta attgggccaa tagtttgaga gccactgaca
    25741 aaaaccttac tttccggaaa tttatcagca agtttgttta ggtaatcgct aaccgtaaat
    25801 gctgtagggt tagtcgttat aatggtgtaa aacccatccg gctgtagggt ttcaccaact
    25861 tgtattacat cgttaagggg gacactcggg cctaaataca caaccttatg gttacgactt
    25921 tttaacaggt agctcaaaaa caacaaactc agttcatgcc attcgccatt tggtaaaaac
    25981 aacacaaact cgcggctttg atcatcatac ttagtggttt gaccatcaat ggccacaagc
    26041 aatttctgcc gaagtagttg ggtcataaaa tgctcctgag cggctataat tgtccctgaa
    26101 acccataaca tacccaattt ctggagatag ggaaaaacaa ccttcttaat ggcatcttca
    26161 aagcccaatt gtaaaataca ggtgttgagt attttctcaa acttttcctc atcaaatgca
    26221 atggtagcaa tggttaggcc ttctatctgg ttattgaatt cattaggggc ctgaatcatg
    26281 gctgtaacca acccggacac ctgatcatgt gtcattttag ccagtttgga gattttataa
    26341 ccatttctat tcagtaacgc cagactcatc aaatatttta gctcagcctc agagtaatac
    26401 cggatgtttg tttctgtacg gctcgcattg agtataccat accgttgctc ccaaatcctg
    26461 attgtatgtg cctttatacc tgagagtgac tccaggtcgc gaatggaata gatagctgcc
    26521 ataatgtaaa gataaattgt ttaatgatat taagtaacag ttaaacaaaa taaaagttgt
    26581 aaaaaaattc aaaatggtga ctttatatct attttaacgg gctaaagcat cgtaactttc
    26641 gataaaatcc aggttatgaa aaaagtactg attcctaccg atttgtctga aaacgctgct
    26701 caaaccttat cttacctctt gccgctggtg attgcacaaa atgccgaggt gcacttagtg
    26761 catgcagttg atgacccttt tgtaaaagaa gagcgcagtg attttgatgg taccggcgtt
    26821 gaaaaataca ctactgatat tttatttaaa atgcgagctg aggctgatgc agatatggaa
    26881 aagtttgcaa ccttatttcg tgaatcttta gcccaagcag gaaaagattt gccggtttac
    26941 agcacagttg aaaatggtct tcctgatgaa ataattttat cgaaagccgc cgccattcaa
    27001 ccacagctta tcattttagg aagacatcac cataatagga tggacagatt gttttttggc
    27061 agtgtgacac aatcagtaat gcaaaaaaca cattttccgg tgctcatttt gccacagcat
    27121 tttgagtatg ctcaaccaaa agaaattctt tatatgagcg atatggatga tcatgatata
    27181 atttctattg gtaaattaat caacttacta caaccctatt ccttcaattt acatattgtt
    27241 cattttaata ttaatgaaat tggtactgaa gaaaaattat ttgcgttagg cgaaaaaatt
    27301 aaaaatgacc atcacgaaat tcctataagt tatgaaaccg tagatggcga aattttgcgc
    27361 gttgcctggc agcagtatat acaaaaaagg ggaattgatt taattgcatt aaccgcacat
    27421 aaacacaatg gcttgcaacg attattaaac aaggatacag cagtagatgt gctttaccac
    27481 agcaatttac cattaatggt gatgcacaaa tattaatttt tgataaaatt accggaaccg
    27541 gaaaaggtag cagcgtttat ttcgtagaag tataatcctg cgggcaggct gtttacatca
    27601 atatgatttg tacctgtttg ctgcattatt gtaaccccgg ccaaattttt aatgcttata
    27661 tcagctgttc cattcatgtc aaaatgcaga acatcgtgca ccggattagg atacacaagc
    27721 atactttttt cagggttttc tattgccaaa gtttcagtta gtcccaaagc atattcaaaa
    27781 gcattggcta agttggctgt atcgagcgga ccaaatccaa atttgttata acataccaca
    27841 tgcgatgacc ttcctgcaac aataattgta ggcattccca ttccgccata attagcagct
    27901 atagcttctc cttcaacaaa ttgagcattg cagatgatgg aattttcagt tttccaatca
    27961 atcaattggc cgcaactata cgaatcatca aaaccgaatg tgtaataatt aattctgtta
    28021 tcgtaattcg cgttataata ttcaattacc ggcgccatat aatgtgcagc attgatgcaa
    28081 ggcatacaac catctaacat cacaatttcc attaaaacaa cctcacctgc atccaattca
    28141 gaaaaaaggt tgtgttcgac gccattgcag tccgtttgag tccaatcctc agcaggaatt
    28201 agctgcccaa atgcagtgcc ggcaaaaagc caaaaactaa aaaaggcaga aatacattgt
    28261 ttcacgctgt aaatttaata attataatta ttaggcctgg tgtaaaccat ttgcacgaca
    28321 ttaatattcc gcatatcgaa tgcggcttcg caataggaga aataataatt ccatttgcgg
    28381 ataaactcat cgtcgaagcc cagttggcgg accttttcga catttaggtt aaacctgttc
    28441 cgccattcag aaagggtaat tacgtaactt ttacccatat cttttaagcc gaacataaac
    28501 aaatcgccgg tttcatttat tgctgtgtta atggcggcaa tgcttgggag taaactgcct
    28561 gggaaaatat gtttttgaat aaaatctacg ttctttttaa aagcttcaaa acgcgaatca
    28621 gggcatacaa ttgcctgaat agccaacaca cctgaaggtt taagtactga ttgtattttg
    28681 tcaaaataca cttttaaata ttgagcccca acagcttcaa tcatttctat agaaacaact
    28741 ttatcatatt tgccttcaat taatcggtaa tcttttaatt gaacttctat taaatgttcc
    28801 aggccttcct gtttaatacg ctgcgttgca aaatcaaatt gttgtttgga aattgtaact
    28861 gtggttattt tacatttata ggtttgcgcc gcatacatgg caaaaccgcc ccagccggtg
    28921 cctatttcca ataaatggtc atcctgattt aatttcaacg attgacacaa actttcatat
    28981 ttttcgaatt gcgctttttc cagctcctgt tttggtgctg tgtaataggc agaagaatac
    29041 gtcattgttt tatcgagaaa taaactaaaa aactcattac tcaaatcgta atgtgctgct
    29101 atattttttt tagaaccggt gacagaattt ttgcgtttac tgtgaaatat cttattggca
    29161 aattgaaatg catttaaaat tagcgtttta gctgttgaac ccgacaatgt tggtgctgta
    29221 tcaacattta gtaaaaacca ggaaagtaat gcagttaaat tagatgttgt ccattcacag
    29281 gccatatacg cttcggcaaa accaatgtcg ccaaataaaa atgcccgctt gaaaaaatta
    29341 tcatcatgta catgcacatc agcatgatgc tgattgcttt tattacccaa ttccattgtg
    29401 ctgccatctg caagtgttat atgcaggtac cccttttgca tgggttgcag acttttcagg
    29461 ataatatttt tataaaactt catattgagt tgccgattaa aattaagttt attttttcac
    29521 attcaaaata tcacgttgca attgcgcatc gtctgatttt ttgtgatatg gtactttttt
    29581 aagccacaat aacagcgctt gccagtgtat tgccgtcatt accctgatgg tgataaaagg
    29641 aaaacgtaat aaataccaga gtaaacgggc atctttcaac acttgtttat cgccggttaa
    29701 agtgctgata aaaaatttat cccctgattt ttccttaaca ttaatttgaa tattgagttt
    29761 tgcatccggt actttgtatc ggaattcgaa ctcggtatcc atatctgtaa atggtgaaac
    29821 gtaaaaatat ttttgtgcat tttgctcaaa tttctccccg ttttttgcat ctaccaaaaa
    29881 caatttcatt tcaccgaaag tgttacttat ttcggtaaca acaaaattac aggtgccttg
    29941 ttcatcataa caaaaataaa agcttaccgg attaaataca taacccaaca tgcgcacatg
    30001 cgtggttaaa aatacttggg ccggcatttt ggttatacca acagacagca aataatcatt
    30061 gagcttttgc cgtgtactta aaccatttcg ggcatcacct gtgggatatt taaaatggtc
    30121 ggtatcccaa aaactataaa tcccacgttt attataggta aataatttac ttgattgagt
    30181 tgtttgttcc aacttgtcaa tatcgagcca aaacataaat
//
LOCUS       DAORKW010000002        28101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3_length_28101_cov_5.265243, whole genome shotgun sequence.
ACCESSION   DAORKW010000002 DAORKW010000000
VERSION     DAORKW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 28101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28101
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3_length_28101_cov_5.265243"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLB46_00145"
     CDS             complement(<1..485)
                     /locus_tag="PLB46_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HRF74947.1"
                     /translation="MNEKSTNRIIFAISVAVPVLVAVLFYTPALNLNINVSFLPKLHA
                     ILNSTVAILLLIGLYFIKTKNIKAHKLTMLGAFSTSALFLISYVIYHSASESTLFGDS
                     NFDGTVSAEESAAVSSIKMVYYIILLSHIILAAVIMPFILITVSHGLSARFDKHRKIA
                     K"
     gene            complement(478..1131)
                     /locus_tag="PLB46_00150"
     CDS             complement(478..1131)
                     /locus_tag="PLB46_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRF74948.1"
                     /translation="MNKRNNIIIAGVVGVAFILFVIFVYLPYADKQENLNKVKQSYQH
                     SSFLRDSLNHIPNFTCVDQNGKTFTNKDVEETVFVAEFFYTECDAYCPVTSKHLAQVQ
                     QSLNNAINFKILSFSLNPETDSVPVLRKYADAYKAKDNMWHFLRGEQSDIFDLGEQGF
                     FTVVKGADGSFKGHSDKFTLVDKNGNIRGFYKGTDSIQMQALIQDINYLVFKNEVNE"
     gene            complement(1115..1771)
                     /locus_tag="PLB46_00155"
     CDS             complement(1115..1771)
                     /locus_tag="PLB46_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRF74949.1"
                     /translation="MANKRGRVWAIVMLTIIVVLPLISVIFLKEGFKIREEAPASSRM
                     IIDNPPQIPDYFTVSHRGDTITKKRMLNKVCVMDFSTYSCGTTNDARERKLFEIQEDY
                     YGKTKALRLISHTLNPELDQTTQLVSMAERYSAREIWHFVRTDDSSSFKFHDFSKNIF
                     EKTGAIETDTLCPQLVYLVDGDGFLRGAYDPLIESQFHDLYNDILFLINKLDLDEQEK
                     "
     gene            complement(1774..2109)
                     /locus_tag="PLB46_00160"
     CDS             complement(1774..2109)
                     /locus_tag="PLB46_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HRF74950.1"
                     /translation="MSHGTHAEKIKKVWTICGVLAIVTAFEIAAALIHWKYMPESPRW
                     WLNLLFIILSAAKAYYIMSEFMHLKYELKAMAVSILAPFLFLVWGIIAFLWEGGYWLS
                     LKESWEILK"
     gene            complement(2128..2805)
                     /locus_tag="PLB46_00165"
     CDS             complement(2128..2805)
                     /locus_tag="PLB46_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRF74951.1"
                     /translation="MEAVAKTNAKELWDGGDSPFRVSYGKVMMWYFLISDTFTFAGLL
                     ISYGAIRMSHPAHEEGNMMSWPDPNEVFNAIPFTHAHLPLAFVTFMTFTLIFSSVTMV
                     RAVQEGHEMNRKGVLFWMFLTILGGLTFVGSQYLEWTHLIHEGQTLTHNPFGAASFGA
                     LFFLITGFHGFHVSVGVCLNTWAFIQTYRGVFHRRGHYEMIEKLGLYWHFVDLVWVFV
                     FLAFYLL"
     gene            complement(2818..3402)
                     /locus_tag="PLB46_00170"
     CDS             complement(2818..3402)
                     /locus_tag="PLB46_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRF74952.1"
                     /translation="MASSIHKRYIVHPYKFNLWIGIVSMVMIFAAFTSAYVVKKGDTR
                     TWEHIHLPSIFTTATIVIVISSLFMHAAYLAFKKGALNLYRGFIVTAFILGAGFLALQ
                     IQGWNTLVNEGYGLTENVAGSFIYVISGAHFLHVAGGVIALMVFAVRAFTRLKTTESD
                     LMKTNTINIEVMATYWHFVDFLWVYLYFFFLLNR"
     gene            complement(3407..4300)
                     /gene="cyoE"
                     /locus_tag="PLB46_00175"
     CDS             complement(3407..4300)
                     /gene="cyoE"
                     /locus_tag="PLB46_00175"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRF74953.1"
                     /translation="MSKETSISKQGIGKTIAAKLKDYHMLVKFRLSFIVVFSAGIGYL
                     FAGGEQSSFFLFLAGGFLITAASNAINQIIESDTDKLMTRTADRPLAAGRMQSTEAII
                     AAGLMAVSGIAILWVWFNTLSALLGALSLISYAFLYTPMKKVSPIAVFIGAFPGAMPP
                     LIGCAAAAGGINDLAILLFSVQFVWQFPHFWSIAWVAADDYAKGGFHLLPSVKGRSKT
                     SALYIVIYTILLIPVSLLMYLLGYTGWISALIVFITGILFLLQTVKLYRSSEIKDARS
                     LMFGSFLYLPIVLIALLVDQI"
     gene            complement(4320..6185)
                     /locus_tag="PLB46_00180"
     CDS             complement(4320..6185)
                     /locus_tag="PLB46_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRF74954.1"
                     /translation="MSVQTSTHTAHADTHGHDHHHDDHHHHQSFIRKYIFSEDHKTIA
                     KQFLVTGIFWAIVGALLSVLFRIQLGYPDADLTWMKPFLGKWITEEGQIAPEFYNALI
                     TMHGTILVFFVLTAGLSGTFSNFLIPLMIGARDMASGFINMLSYWFFFISGAVMFVSF
                     FLATGPASGGWTAYPPLNALKDASSGSVVGVDYWLISMALFIVSALLGGLNYITTIIN
                     LRTKGMKMFRLPLPIWAFLLTAVIGVLSFPVLLSAAVLLLFDRNLGTSFYLDAINLGG
                     EPLANSGGSPILYQHLFWFLGHPEVYIIIIPAMGIVSEVLSVHSRKPIFGYTAMIYSI
                     LAITVLSFIVWAHHMFMTGLNPFIGSIFVIFTLIIAVPSAIKVFNWLTTLWKGDIRLN
                     PQMLFAIGFVSLFISGGLTGIFLGNSSIDIRLHDTYFVVAHFHLVMGVAAFFGMFAGV
                     YHWFPKMFGRYMNQTLGYIHFWGTIVSAYLIFYPMHFEIALPRRYYTHSGFETFSGFE
                     SLNMFISTVAIISFALQLLFVFNFFYSIWYGRKMRDKNPYNANTLEWTTPLRPGHGNW
                     EGDIPEVHRWPYDYNKHGREFIPQTEPLSQYEIEHGEHGDEMDGDHIIDDKDLKS"
     gene            complement(6210..7640)
                     /locus_tag="PLB46_00185"
     CDS             complement(6210..7640)
                     /locus_tag="PLB46_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HRF74955.1"
                     /translation="MATGWLIFIAIVMIVVVLFQVTRTLDLVGQLRGGERTTVEMTKF
                     HAILGIIFMVLGMIGFFWSFGHYADRMVDHHSSDNGKMIHKMFVVTLWVTGIVFVITN
                     VLLFVFAYMYRNRPGNEAVHFAHSNKLEFIWTIVPTIVLTGLVVFGLQAWTDIMGDPE
                     EDAITIEVTGQQFFWSSRYTGPDGKLGPRDNDLICPENPLGIVTREWIEHRIVSLKGS
                     EKLNQKGEIVKLQEREKEINAQLAIYYDIVNNSPNKYRVAVVKDSVDLLETELNKIPK
                     KIDIREKAFTRITTKYTEEYIAANADEFTWGYDDPMPSEIHVPVNNQVYVKITALDVL
                     HNFYIPHMDVKMDAVPGMPTSFTFTPLITTADKRIEMSENDAWKVIEEGDDMPRWQKF
                     NYEIACAELCGTGHSSMKYTLVVDSPEDYDKWLAEQPSKWSQVYASLYLENGVSKYAP
                     KIAAPAAAADTVTIANTDVTVVVNKN"
     gene            complement(7650..9050)
                     /locus_tag="PLB46_00190"
     CDS             complement(7650..9050)
                     /locus_tag="PLB46_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74956.1"
                     /translation="MNGSNKFEYNSKLKTRFLGLLIVGIVLTVVGMATGASMDRFWSN
                     YLLDTILFLGISVLAIFFLTAHQIAMSGWHIMVKRVPEAMSQFSKVGAIFMLVIIVGL
                     WAGYHNLYAHWDNPFVKEKMVTLEELEHYETEHADHLGGHEKDYSRVWQEFQYDGKAE
                     VEAHHGDEHHATEHKVELVANDQGQVVNPHYDRLIAEKHGYLNKSFWTIRSIAYLLLW
                     VGVAITLRKFSLKEDLEGTTKWFLRTKFWSAIFLIIWAVTSSTMAWDWVMSLDPHWYS
                     TLFGWYNFVSLWIACLCTIVLILIYLKRNGYLAELNENHLHDLGKYIFGFSVFWTYLW
                     FSQFMLYWYGNIPEETRWWLDRARTNYKGIFYANLIINFLFPFLTLLRRDAKRNLNVL
                     TIVASVLILSHWLDFFLMIMPPTVGDHWGIGFLEVGLFMTFAGLFLYLVFGELAKASL
                     VPEKHPLYRESLDHHI"
     gene            complement(9096..9770)
                     /locus_tag="PLB46_00195"
     CDS             complement(9096..9770)
                     /locus_tag="PLB46_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRF74957.1"
                     /translation="MVASKYIKTAVVITALPLVFASCKKAGGDYAGDSYTWDMNYSRA
                     YETYSMMPEMPNGMSAVTPVDGTVPYVGNAIAGVKSDSLTMALNLPYQLPNTPEGYSR
                     AALEVKNPFNINDKEVVAEGKHFYDIYCAVCHGAAGDGKGFIVTEGKYTAMPPNYFDP
                     VYMAMTEGTMFHSVTYGRNAMQPYSYALSKEERWKVVTYVKHLQAEYAAKQLPSAASD
                     TTASIK"
     gene            complement(9792..10319)
                     /locus_tag="PLB46_00200"
     CDS             complement(9792..10319)
                     /locus_tag="PLB46_00200"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HRF74958.1"
                     /translation="MANKKIIYGLYDDEVILIDAVKKVRAEGLKIHDCYTPFPVHGLD
                     DAMGLRMTKLHSAGFIFGATGTLTALTFILWVTTNNYPINYGGKPYLSLPAWIPITFE
                     LTVLFASVGMFLTYLYLNRLAPGVKAEIVDERTTSHLFAMTFELDEHTSDSKKEEIRK
                     VLAASGAVEIHEKEL"
     gene            complement(10349..11749)
                     /gene="nrfD"
                     /locus_tag="PLB46_00205"
     CDS             complement(10349..11749)
                     /gene="nrfD"
                     /locus_tag="PLB46_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HRF74959.1"
                     /translation="MFVSPLREPLIQGHKTYKQISDDICAPVEAAPSKAWMIAVTLSG
                     LLALTGFTMIGITITYGIGMWGLNKTVGWAWDITNFVWWIGIGHAGTLISAILLLFRQ
                     KWRTGVNRSAEAMTIFAVICAGLFPIIHMGRVWLAFFIFPYPNTRGPVWVNFNSPLLW
                     DVFAISTYFTVSLVFWYTGLIPDLALVRDRTTSAFKKRMYNIFSFGWKGTAKNWQRFE
                     SISLVLAGLSTPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVLTLM
                     LVVRKVFNLQEYITLAHIESMNKVIILTGSIVGVAYITELFTAWYSGNQYEQYAFLNR
                     STGPYWWSYWGMMTCNVVSPQLFWIKKLRRNIVFTFFMSIFINVGMWFERFVIIVTST
                     HRDFIPSSWTMYYPTIVEIFIYLGTIGFFMTLFLIFAKVAPVIAVAEVKSILKTSGDQ
                     YTGPDAIHHHDDHHKH"
     gene            complement(11760..14879)
                     /locus_tag="PLB46_00210"
     CDS             complement(11760..14879)
                     /locus_tag="PLB46_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HRF74960.1"
                     /translation="MAEKKYWKGLEELNQAPESAQLIQPEFGEEIPVEFFDSSNDGLH
                     SNRRDFLKMMGFSLTAATVAAGCEIPVKKVAPYIFKPEDVIPGIATWYASTFYNGGDY
                     CSIIVKTREGRPIKIEGNRDSKVTKGGTSARVQASVLSLYDTARLRNPMVKGKRGHDN
                     ASWADIDKAIAGKLAANPNANIRIVSSTVISPSTKKVFAAFAEKYPNTKVYTYDALSY
                     SGMLMANMASKGKSEIPDYHFEKAKVIVSIDADFLGTWISPIEFTKGYVATRKVTKEK
                     PEMSRHYHFESRMSLTGSNADYRVAVAPSELGVVALNLLDAITGSSTAGGAKLKDEAK
                     QARLKEAAEWLKKHRGESLVVSGCNDENVQLVVNAINDALNNYGNTITWDSANMLRQG
                     SDASIKTLIGELKGGQVNILMVNDCNPVFDHPMGDQLAEAMTKAEMSVSFAGSMNETA
                     ANCTYICPDHHFLEAWNDAEPKRGLYSLSQPTIKNLFDTRQMQDSLLTWSGNAMTYHD
                     FMVQNWVTGPLAGKADAMGAFNNALQIGLYQPENGNVIEAVVATLTAATEMAPATTDV
                     SSAAANILGMAKKASGVEIQFYESIAIGDGRYADNPWLHELPDPVSKITWDNYLNVSP
                     AYARENKIEHGDVVTVSVNGKEVKLPVAIQPGQANNTVSVAVGYGRTMVGKGGAGVGV
                     NVYHLLDASGACVNYMAANVTITNTGENVKLAQTQTHFSLNDGLGQRRIVKETTLAEY
                     KKSPNAGNEDRAEALAWKDITFYPDYFGKKNGFNWSMAIDLNSCVGCGACVISCNAEN
                     NIPIVGKNEVIRSREMHWMRIDRYYAGTEENPEVVYQPMLCQHCENAPCENVCPVGAT
                     NHSSEGFNQMAYNRCIGTRYCANNCPYKVRRFNWFDYGGADTFGTMNDAKETENRGLL
                     EDLTRMVLNPDVTVRSRGVIEKCSFCVQRIQEAKLGAKKENRELKDGEFRVACQSACP
                     ADAITFGNSYDDTSAVVSEAKDERTFGILEEFHWVPSVFYKTKVRNKDKADHKNEEAL
                     DIMDLYNYK"
     gene            complement(14921..16177)
                     /locus_tag="PLB46_00215"
     CDS             complement(14921..16177)
                     /locus_tag="PLB46_00215"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRF74961.1"
                     /translation="MQHKKSTTITRFLSIILLLALSFTANNVFAGEAEGKVLFQSKCQ
                     RCHLVDKKLTGPALQNVRTRWADSTNLYAWIKNSQSFLGTGDKYANDLYKEYNSIMPA
                     FPELSDADIADMLVYIDAEAKRKQELAAAPGPGGVVTETKKDNSTLYWILIVALLFIA
                     VILGRAVKYLDGLVRQKFDDPTPDTTPFYKQRKYRPLVGFLALLFLSLLSVAMWNSAT
                     SLGRQQGYAPEQPIAFSHKVHAGINKIDCKYCHVGAERGKAAVIPSLSVCMNCHYNIQ
                     GVTGTDPNYPKAVYDKEIAKIYSYIGFNTESMKYESEPKPIEWTKIHNLPDHVYFNHS
                     QHVKVAGLECQTCHGQIQEMDVVQQMENLSMGWCVNCHRNTNVNFTTNEFYKPYEVLH
                     EKIKNGEMESVKAEDIGATECQKCHY"
     gene            16461..17636
                     /locus_tag="PLB46_00220"
     CDS             16461..17636
                     /locus_tag="PLB46_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74962.1"
                     /translation="MKKLVYASMLLLVISSGCKVAEKEFREGNYDEAINICIKKLIDN
                     PDKVEYILLLEEAFVRANGEDLDYIKALNTDGSPDRWEEIYNVYEKIYGRQLKIKRLL
                     PLYVGTEERDAEFKFADALDGMNTAKKNAAAYWYADASLKLKSNDVYKAREAYYELQK
                     IERFYNDYKDADDLMKKAKEIGTNDVVFKVENMANASLNYDVTKAINQINIPKSSGSW
                     YIVHSSVFEKGVEMTIQLNIRKIEAYPEKVSTNQYEESKQIIDGYEYVYDNQGNIVKD
                     SLGNPIKEPDYKTVTAYVSETWQEKVATVSGEVVYLNKNGEVIRSFPVRADAVFKNYY
                     AAATGYAEALTPQSKEKLGGKPLPFPYDDQMLIDAVNLLENEVRSITNANNDEYLNL"
     gene            17743..20088
                     /locus_tag="PLB46_00225"
     CDS             17743..20088
                     /locus_tag="PLB46_00225"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF74963.1"
                     /translation="MNIKRFAQFSCLIFFIVLSGNNLFAQSYTISGYVTEKGSGEKLL
                     SATVFNSKNFSGTSTNDYGFYSLTLPGDSVIINYSYIGYITQQKKFVLKKDTVLNIAL
                     EQSNELKEVVIEGTKSDEQFQEKTQMSTIEIPIEQIKALPAFLGEVDVLKALQLLPGV
                     NGGSEGSSGLFVRGGSPDQNLILLDGVPVYNASHLFGFFSVFNSDAINKVTLVKGGFP
                     ARYGGRLSSVVDIRMKEGNMNSYHGEGSIGVIASRLTIEGPIIKDKTSFIVSGRRTYL
                     DLITRPIMKSQQDEEGVSGYYFYDLNAKINHKFSDKDRLYVSGYFGRDKAYFNYDYQY
                     DETTQRVEDNGIYWGNATAVARWNHMFSNKIFSNLTTTFTDYKFDIYSTVTEIKTDDP
                     DTYFGFDYFSGIYDWSLKYDIDYVPNPKHYIKFGAGVTYHTFEPGATQFESENIQEFE
                     DAFSFGSEKVYASEFDVYIEDDWEVTNKLKINGGLHASGFLVNGLFYKSLQPRISSRY
                     LVNPNLSIKASFSTMTQYIHLLTNSGIGLPTDLWVPATDSVPPQSAIQPALGVAYTLN
                     NQYEFSIEGYYKKMNDIIEYKDGANYFFDGLENWEDKVESGEAWAYGAEFFIQKRTGK
                     LSGWIGYTLSWSNRQFATINLGEKFPYKYDRRHDFEIAGIYKINDHIELSATWIYTSG
                     QPVSLPIAKYEGAGGQTLYAYEGRNGYRMNAYHRLDANVALSKEKKYWTRTWNFGVYN
                     AYSRLNPFFIYQAYDYKTNTNNYRQVSLFPIIPSVSYEFTF"
     gene            20114..21064
                     /locus_tag="PLB46_00230"
     CDS             20114..21064
                     /locus_tag="PLB46_00230"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HRF74964.1"
                     /translation="MKNNIITIVAISGLVFLAACEKTVYFDVETKENRLVVNSFLQPD
                     STNRAVVSLSVDPLAIGFESERVSDATIAISKNGSFIGNFTHEFNGDYYIENDLLHAE
                     PNDEFELTVSAPGKETVTASTTIPSLIPITSAAIIDTFLIEISYSSIDPYGNVIYIDT
                     LVPYFKMAITFTDPGTEDYYSLAVMYKDIFNESFACVSTNDPIFTVSGEYNFGAENQA
                     GAATFCDEVMFTDVTFNGTTKTVELSIYAINTIGIADPRFEFSLKHVSKEYFQYYTTT
                     EKQLENEGNPFGEPVIIYSNIENGFGIFAGSTLSKAIIEL"
     gene            21069..21800
                     /locus_tag="PLB46_00235"
     CDS             21069..21800
                     /locus_tag="PLB46_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74965.1"
                     /translation="MLNPNKQSGLLFDGMAILTGLIMFTRVKEYFYNNYTAIGPLPWE
                     ITCFITIGVICYTLGLWFYKNHFLKNISETKEKKHGVVHQISLLVLTLMHFAIFCVLA
                     VVIADSWQVVFPSNFLRFVFYGLIGGLPNLILIWTFYRYQTNQSNYEINNINITIGNI
                     LLSISAIFIQVLFWNVMTIPMLQNLQVESFYGRVIFAGLFFFLFLLIYLPPRLFYFIV
                     DYPYTRTWMQITLAYLPTGLYIITK"
     gene            21901..22605
                     /locus_tag="PLB46_00240"
     CDS             21901..22605
                     /locus_tag="PLB46_00240"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRF74966.1"
                     /translation="MKKNYVLATCLFLIAPLLHGQVAADEIKKTPYIEVTGSAEMEIV
                     PDEIYIQVTLQERYNGKEKITMEKQDALFITGMQSLNIDSKDISLADAQSDFSSYKWK
                     KEDALARKSYVVLVHDAALVSKVYAKLVEIDAEDAYILKTSHSKIEEYRKQIKINAMI
                     ATKDKAGYLLSSIGEEVGSPILIQERETYDSPYTISQRSLASNVAFEAYNLNASDIGF
                     KKIKLRYEIFAQFEIK"
     gene            complement(22644..24281)
                     /locus_tag="PLB46_00245"
     CDS             complement(22644..24281)
                     /locus_tag="PLB46_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF74967.1"
                     /translation="MKKLTVLCIAILVIQISQIEAQQAIWATSFAEAELYASTVDNEG
                     NVYAGGLFYSTISLDGFELTNGTPNAAGWITKHDKNGTPKFLELVYATEYLVVLDMEP
                     LAEGGILICGIFIGKAWLGDFIFGNESDNNYRGFVAKYNADGECTAAYASDETQSVYF
                     YALSQDEEANIFIAGAAEGFFDFGVTEVDHSGDSDAIYGKMEADLNPLWMKSIGGADY
                     DFANEITVDVDGNIIISGFHEGMFEEDSYFFDSYGSSDIFVVKTDANGFIEWNQNMGS
                     AAGDGYLFADLETDEAGNIYYATTMGETLNYIGGSINSYGEADMVMMKLAANTGNVTW
                     ILHGGGTDSDPMNKMIKGHNNNFYVCGYFYGAAEFQGLQLYAEVEADGYLAEISEGGT
                     VNVVSQTDGDNIEAYQSLAIDENNNLLLSGYFFGNLNFDGIDPIASVSEEIDIFIVKM
                     ETPYTLPEVAIQNWNNLTNAIITPNPATSTIVMEMENYNDNFTITFFNSNGQQIAVIN
                     TTSNTPFNVNEFPAGIYTALATNKKGELIASQKFIKQ"
     gene            complement(24443..25327)
                     /locus_tag="PLB46_00250"
     CDS             complement(24443..25327)
                     /locus_tag="PLB46_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF74968.1"
                     /translation="MINNTQLQSKLNNYAAFATAFLFTHNLNAEIVYTDIEPDITLNN
                     EDYYYEIDIDNGGVNDFQLLRINGVYYSYWESVYRSFTALFFDNLGENKFFAGSYFTV
                     GSAMYSSYQTFRPYAIPVDYSVGPLLSFQSEGWPLMVSHVFDESHNLIKNVGHWSAVG
                     EAYIGIRMGVLGEAHYGWIRVTIIEEPLSMIIHDFAYETTLNKPIRTGATFNSVAEIE
                     SGSSPFSIVNFGNNTIQIITPNQLPYTTQLFDLSGKIVFTSNQQIGNSTITIPPQTSI
                     YILQIQSEGNQYGFKIHL"
     gene            complement(25324..27225)
                     /locus_tag="PLB46_00255"
     CDS             complement(25324..27225)
                     /locus_tag="PLB46_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF74969.1"
                     /translation="MKPMSPHSLYHCYQNFLKAKISAWSIFFYLLLFHLNLSAQAPDW
                     SWATYIHGTSNDAIQFTDVDNKGNMLLAGPFYSPALQIGDTLVISNSNVSSTGSYLAI
                     IDSSGKTIWAKQLYAESEYSTYVKINDVIFDAVGNIILTGTSYSENIILDDLYLESIE
                     EGYFSFIIKLSPTGEVMYSHVFEKGAIPTSLVSDLFGGWYVTAHQYYYNEGVDLGEIQ
                     LDANVNGNRMFVAHYTSANIVDWAEVFRGDCDDLLGVETTNGDLIFYGEFSGATFKVD
                     TFLLIDSSYHASNFLGKCDVYGNFSWARLLSPQSVYVKDAVTIPNGIMLVGWLNDELA
                     IFDSDTIVHGEEINTTFMAKFNLNGELISATESSSFFPGYATINVSPDNLFYVSAQVY
                     NSLWTGVDTIFNETNSGADLAVLVYDTAQLPISQFVIPLDGDNDIPKVEWDIFGNMLL
                     IGAFSEDTLIFNTDTLINIQLSNQADYFVARSNTCTTQLFPIAIEGLVLSAVEGVYWQ
                     WYLNGLPIKGANSQYYTPLQNGTYMVSVKQANGCTLWSETALLPEQIFSDEFDFTIYP
                     NPATDEIHVFIPFAYESAAIYNALGQLIYQFDDTNQTNLSYANDLKGMYIFVVSANGK
                     TQSHQFIIL"
     gene            complement(27302..27550)
                     /gene="atpC"
                     /locus_tag="PLB46_00260"
     CDS             complement(27302..27550)
                     /gene="atpC"
                     /locus_tag="PLB46_00260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRF74970.1"
                     /translation="MQLEILSPDKKIYSGEAVGVQMPGVTGSFEVLNNHAPMIAALTT
                     GKMRVNTGKENVVYNIKGGFVEILNNNVSVLVEGVELA"
     gene            complement(27581..>28101)
                     /locus_tag="PLB46_00265"
     CDS             complement(27581..>28101)
                     /locus_tag="PLB46_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRF74971.1"
                     /translation="VQAVYVPADDLTDPAPATTFSHLDATTVLNRKISELGIYPAVDP
                     LDSTSRILSADIVGEEHYNCAQRVKSILQRYKELQDIIAILGMEELSEDDKLVVSRAR
                     RVQRFLSQPFHVAEQFTGLKGVLVPIEETIRGFNMIMDGEVDEYPEAAFNLVGTIDDA
                     IEKGKRLIAEAK"
BASE COUNT         9146 a         5376 c         4965 g         8614 t
ORIGIN      
        1 attttggcaa ttttccggtg tttatcgaac cttgcagaca acccatggga aacagtaatt
       61 aaaataaacg gcatgataac agcagccaga ataatgtgcg atagtaaaat gatataatac
      121 accattttta tactgctcac tgcagcactt tcctccgcgc taactgttcc atcaaaattg
      181 ctatcgccaa ataatgttga ctcagaggct gaatgataaa tcacataaga tattaaaaac
      241 aatgctgagg ttgaaaatgc tcccaacata gttaatttat gcgccttgat atttttcgtt
      301 tttataaaat acaaaccgat taataataaa atggcaacgg tagaatttaa tatggcatgt
      361 aattttggta aaaatgaaac attgatattt aggttcaatg caggtgtata aaataatact
      421 gcaactaaaa ccggcacggc caccgatatt gcaaaaatta ttctattggt tgatttttca
      481 ttcattgact tcatttttaa acacgagata attgatatcc tgaattaaag cttgcatttg
      541 aattgagtcg gttcctttat aaaatcctct gatattacca tttttatcga caagcgtaaa
      601 cttatccgaa tgccctttaa acgagccatc cgctccttta acaacggtaa agaaaccttg
      661 ttcacccaaa tcaaaaatat cggattgttc tccacgtaaa aaatgccaca tattatcttt
      721 ggctttatac gcatcagcat attttcttaa aaccggcact gaatctgttt cagggttgag
      781 gctaaaagaa agaattttaa aatttatggc gttgtttaac gattgctgta cttgtgcaag
      841 atgttttgat gtaaccggac aataggcatc acattcggta taaaaaaatt ccgctacgaa
      901 tactgtttct tcaacatcct tattggtaaa ggtttttcca ttttggtcta cgcaggtaaa
      961 attaggaata tgattaagcg agtcgcgtaa aaaagaagaa tgttgatacg attgttttac
     1021 cttattcagg ttttcctgct tgtcggcata aggcaaatac acaaaaatca caaagagaat
     1081 aaaagcaact ccgacaacac cggcaattat tatattattt ctcttgttca tccagatcca
     1141 gtttatttat cagaaacaga atatcgttat ataaatcgtg gaattgggat tcaatcaaag
     1201 ggtcgtaagc gccgcggaga aatccatccc catccacaag atatactaac tgtggacaaa
     1261 gcgtatcagt ttcaatagca cctgtttttt cgaaaatatt tttactgaag tcgtgaaatt
     1321 tgaatgagga gctgtcatcc gttctaacaa agtgccaaat ttcacgggct gagtaccgct
     1381 cagccataga cactaactgg gttgtttggt caagctcagg gttaagcgta tgtgagatta
     1441 gacgcaatgc ttttgttttc ccataatagt cttcctgaat ctcaaataat ttccgctcgc
     1501 gggcatcatt agtagtaccg cagctgtagg tagaaaaatc catcacacat accttgttaa
     1561 gcatacgttt tttggtaatg gtatctcctc tatgagaaac agtaaaataa tcaggaattt
     1621 gaggtggatt atcaataatc atcctgctgc ttgcaggtgc ttcttctctg attttgaagc
     1681 cttcctttag aaaaataact gaaataagcg ggagcacaac tataattgtg agcattacaa
     1741 ttgcccatac ccgcccccgt ttgtttgcca tcgttattta agaatctccc acgattcttt
     1801 taaactcaac cagtaaccac cttcccaaag gaatgcaatt ataccccata ccaaaaacag
     1861 gaacggtgct aatatactta cggccattgc tttaagttca tattttaagt gcataaattc
     1921 gctcatgata taatacgctt tagccgcact caaaataatg aagagtaagt taagccacca
     1981 tcttgggctt tccggcatat acttccagtg aataagcgcc gctgcaattt caaaagcagt
     2041 tacaattgca agaacaccgc aaattgtcca tacttttttt attttttctg catgagtacc
     2101 gtgtgacata tctttcagtt aaatttttta taataaatag aatgctaaga atacaaaaac
     2161 ccaaactaaa tctacaaagt gccagtataa acctaatttt tcaatcattt cataatgtcc
     2221 acgacggtgg aaaacccctc tgtaagtttg aataaatgcc caggtattta aacacacccc
     2281 aacactcacg tggaatccgt ggaaaccggt aatgaggaag aacaaagcac caaacgatgc
     2341 tgctccaaaa gggttatggg ttaaagtttg accttcatga attaagtggg tccattcaag
     2401 atactgacta ccaacaaagg ttaaaccacc aagaatagtc aggaacatcc agaataaaac
     2461 accttttctg ttcatttcat gtccttcctg cacggcacga accatggtaa ccgagctgaa
     2521 aatcagcgta aaggtcatga acgtaacaaa tgccaaaggt agatgcgcat gtgtaaatgg
     2581 aatggcgtta aatacttcgt taggatccgg ccaactcatc atgttacctt cttcatgagc
     2641 aggatggctc attctgatag ctccataaga aattaataag ccggcaaaag taaaagtatc
     2701 gctgatgagg aaataccaca tcatcacctt gccatagcta actctaaacg gggaatctcc
     2761 accgtcccaa agttcttttg cgttcgtttt agcaactgct tccaaaattc taaaatttta
     2821 tctgttcaat aaaaagaaaa aataaagata aacccataaa aaatcgacaa aatgccaata
     2881 agttgccatt acttcgatat taatagtatt tgtcttcatt aaatccgatt cggttgtttt
     2941 taatctggta aaagcacgaa cagcaaacac cattaatgca attacaccac cggcaacatg
     3001 caaaaaatgc gcgccggaaa taacataaat aaaagaacct gcaacatttt cggttaatcc
     3061 gtaaccttca ttaaccaaag tattccatcc ttgaatttga agggctaaaa aaccagcacc
     3121 taaaatgaat gctgttacaa taaaccctct atataaattt aaagcacctt ttttaaacgc
     3181 caaataagcg gcatgcataa aaagactgct aatcactatt acaattgttg ccgttgtaaa
     3241 aatggatggc aaatggatat gttcccaagt gcgtgtatct ccttttttta caacataggc
     3301 gctggtaaat gcagcgaata tcatcaccat cgacacgatg ccaatccaca agttaaactt
     3361 gtagggatgt acgatatacc ttttatgtat tgatgatgcc atatttttaa atttgatcta
     3421 ccaacaatgc aataagcacg attggtaaat ataaaaacga accaaacatt aaactgcgtg
     3481 catctttaat ttcactgctg cgatacagtt taacagtttg taataaaaat aatattccgg
     3541 ttataaaaac aattaatgct gatatccacc ctgtgtaacc taataaatac ataagcaaac
     3601 ttacaggtat caataagatg gtataaataa caatatataa agctgatgtc ttagaacgtc
     3661 cttttacaga aggcaataaa tgaaatccgc cttttgcgta atcatcagct gcaacccagg
     3721 caatagacca aaaatgagga aactgccata caaactgcac tgaaaataac aatatagcta
     3781 aatcgttaat tccacctgct gctgctgcac aaccaataag tggtggcatt gcaccgggaa
     3841 atgcgccaat aaaaacagca attggtgaaa cctttttcat tggtgtatac aaaaaggcat
     3901 aactaatgag tgataaagct ccaagtaatg cacttaaggt attaaaccaa acccataaaa
     3961 tagcaatacc cgaaactgcc atcagcccgg cagcaattat agcttccgtt gactgcattc
     4021 ttccggcagc taaaggtcga tcagcggtgc gcgtcattaa tttatccgta tcagattcaa
     4081 ttatctgatt aatagcattt gatgctgctg taatcaaaaa tccacctgct aaaaataaaa
     4141 agaacgatga ttgttcgcca ccggcaaata aatatcctat acctgcactg aacacaacaa
     4201 tgaaactcaa tctgaatttt accagcatgt ggtaatcttt cagtttggct gcaattgttt
     4261 tgcctatgcc ttgtttagat atagaagttt ctttgctcat gtaattatga aaaagctaat
     4321 tatgatttta aatctttatc atcaataata tggtcgccgt ccatttcatc accatgttca
     4381 ccatgttcaa tttcgtattg acttaacggt tcagtttgag gaataaattc tcttccgtgt
     4441 ttgttatagt cataaggcca acggtgaact tcaggaatat ctccttccca gttaccatgt
     4501 cccggacgca aaggtgttgt ccattctaat gtatttgcgt tgtaaggatt tttatcacgc
     4561 atttttctac cataccaaat actatagaag aaattgaata caaataacaa ttgtaatgca
     4621 aatgaaataa ttgccactgt cgaaataaac atgttcaaag attcgaaacc actaaatgtt
     4681 tcaaaacctg agtgggtata ataacgacgt ggtaatgcaa tttcaaagtg cattggatag
     4741 aatatcaaat aagcagatac gattgttccc cagaaatgaa tataacctaa agtttggttc
     4801 atatagcgac caaacatttt agggaaccag tggtaaacgc ctgcaaacat tccgaaaaat
     4861 gctgccacac ccattactaa gtggaagtga gcaacaacga aataggtatc gtgtaaacga
     4921 atatcaatag aagagttacc taagaaaata cctgttaacc ctccggaaat gaacagtgaa
     4981 acgaagccaa ttgcgaacaa catttgcgga tttaaccgaa tatccccttt ccataatgtg
     5041 gtaagccagt taaatacttt gatcgcagat ggcaccgcaa taattaacgt aaatattacg
     5101 aaaattgaac cgataaatgg attcaatcct gtcataaaca tgtggtgagc ccaaacaata
     5161 aaggataata ccgtaatggc aaggattgaa taaatcattg ctgtataccc aaaaattggt
     5221 ttacgcgaat gcactgacaa tacttccgat acaataccca ttgccgggat aatgataata
     5281 tacacttcgg ggtggccgag gaaccagaat aaatgctgat ataaaatagg gctaccacct
     5341 gagttagcga gtggttcacc accaagatta atcgcatcta aataaaagct agtacctaaa
     5401 ttacggtcaa ataataataa tactgccgca gataataata ccgggaatga caataccccg
     5461 ataacagcag tcaataaaaa cgcccatatt ggtaaaggca atctgaacat cttcatgcct
     5521 ttagtacgta aattaataat agtagtaata tagtttaagc cccctaataa tgctgaaaca
     5581 ataaagagtg ccatagaaat caaccagtaa tcgaccccaa caacacttcc cgatgatgca
     5641 tcttttaatg cattcaatgg tggatatgcc gtccaaccac ctgatgcagg acctgttgcc
     5701 aaaaagaatg atacaaacat gacagcgccg gaaataaaga agaaccaata agacaacata
     5761 ttgataaatc cagaagccat atcgcgtgct ccaatcataa gtggaattag aaagttggag
     5821 aacgttccac tcaaaccagc agttaataca aagaatacca aaatagtacc atgcatcgta
     5881 attaatgcat tataaaattc cggagcaatt tgtccttctt cagttatcca ttttcctaaa
     5941 aatggtttca tccatgttaa gtcagcatcc ggataaccga gctgaatacg gaataaaaca
     6001 gataataaag ctccaacaat tgcccagaaa ataccggtaa ctaaaaattg ttttgcaatt
     6061 gttttatggt cttcactaaa aatatatttg cgaatgaagc tttgatgatg gtgatggtca
     6121 tcgtggtgat gatcatgacc atgtgtatct gcatgcgctg tatgagttga agtttgtact
     6181 gacatatctt tctatttacg ttccaccaat taatttttgt taactactac agtaacatct
     6241 gtatttgcaa ttgtaactgt atcagctgct gctgccggag ctgcaatttt aggtgcatat
     6301 tttgaaacac cgttttctaa atataatgat gcatatacct gactccattt gctaggttgt
     6361 tctgcaagcc atttatcata atcttccggg ctatcaacaa ccaaagtata tttcattgag
     6421 ctatgtcctg ttccgcataa ttcggcacaa gcaatttcgt aattgaattt ttgccatctt
     6481 ggcatatcat caccttcttc aataactttc catgcatcgt tttctgacat ttcgatacgt
     6541 ttgtcggccg tagttattaa tggagtgaaa gtaaatgatg ttggcatacc cggaacagca
     6601 tccattttaa catccatatg agggatataa aaattgtgta atacatcgag tgctgtaatt
     6661 tttacataca cctgattatt tacaggaaca tgaatttcac ttggcatagg gtcatcataa
     6721 ccccaggtaa attcatctgc atttgctgct atatattctt ctgtatattt agttgtaatg
     6781 cgcgtaaaag ctttttcacg gatgtcgatt tttttcggga ttttattcaa ttcagtctcc
     6841 aataaatcaa ctgaatcttt cacaacagca acgcgatatt tatttggcga attatttaca
     6901 atatcataat aaatagccaa ttgcgcattg atttcttttt cgcgttcttg taatttaaca
     6961 atctcacctt tttggttcaa tttttcactt ccttttaagg aaacaattct atgttcaatc
     7021 cattctctgg taacaattcc aagtggattt tcagggcata ttaagtcatt atcacgcggt
     7081 cccaatttcc cgtccgggcc tgtataacgg ctgctccaga aaaactgttg accggtaact
     7141 tcaatcgtta tcgcatcttc ttctgggtca cccataatat ctgtccaagc ctgtaatccg
     7201 aaaaccacta atccggttaa cacgatagtt ggaacgattg tccaaataaa ttcgagctta
     7261 ttgctatgcg caaagtgcac tgcttcgtta cccggacgat ttctatacat gtatgcgaaa
     7321 acaaataata gcacattagt aataacgaaa acaattccgg ttacccaaag tgtaacgaca
     7381 aacattttgt gaatcatttt tccattatcc gatgaatggt gatctaccat tctgtcggca
     7441 taatgtccaa atgaccagaa aaatccaatc atacccagaa ccataaaaat gatccctagt
     7501 atagcgtgaa atttggtcat ttccactgtt gtgcgttcgc cgcctcgtaa ttgtccaaca
     7561 aggtcgagtg ttcttgttac ctggaacaag accacaacaa tcataactat tgcaataaat
     7621 atcaaccaac cggtagccat tgtataaatt tatatgtgat gatcaagact ttctctgtat
     7681 aaaggatgct tttcaggcac cagtgacgct ttagcgagct caccaaaaac tagataaagg
     7741 aataatcctg cgaaggtcat aaataaacct acttcaagga atcctattcc ccaatgatcg
     7801 ccaactgttg gaggcataat catcaggaag aaatctaacc aatgtgaaag tattaataca
     7861 cttgccacaa tagttaaaac atttaaattt cgttttgcat cgcgacgtaa tagtgtcagg
     7921 aatgggaaca agaaattaat gataaggttt gcataaaata tacctttata attagttctc
     7981 gcgcgatcta accaccaacg tgtttcttca ggaatatttc cataccagta taacataaat
     8041 tggctaaacc ataagtaagt ccaaaataca ctaaaaccaa aaatatattt acctaaatcg
     8101 tgtaagtgat tttcatttaa ttcggcaagg taaccattgc gttttaaata aataaggatt
     8161 aaaacgatgg tgcacaaaca agcgatccaa agtgaaacga agttatacca tccaaacaag
     8221 gtgctatacc agtgtggatc taacgacata acccagtccc aagccattgt agaacttgtt
     8281 acagcccaaa taattaaaaa tattgctgac cagaatttag tgcgtaaaaa ccattttgta
     8341 gttccttcta aatcctcttt aagtgaaaac ttgcgcaggg taatggcaac accaacccat
     8401 aataataaat aagcaataga acggatggtc caaaatgatt tatttaaata accatgtttc
     8461 tcagctatta atctgtcata gtgtggattt acaacctgac cttgatcatt cgcaactaat
     8521 tcaactttat gttccgttgc gtgatgttca tctccatgat gtgcttcaac ttctgcttta
     8581 ccatcatatt ggaactcctg ccatacacgt gaataatctt tttcatgacc accaagatga
     8641 tcagcatgtt cggtttcgta atgttctaat tcttctaatg ttaccatttt ttctttcaca
     8701 aatggattat cccaatgtgc atacaaatta tgataacctg cccaaagacc aacaataatc
     8761 acaagcatga agattgctcc aaccttcgag aattggctca ttgcttcagg aacacgttta
     8821 accatgatat gccagcctga cattgcaatt tggtgtgccg ttaaaaagaa aattgccagc
     8881 accgagatgc caaggaataa gatggtatcc aacaagtaat ttgaccagaa gcgatccatt
     8941 gaagcccctg tagccatgcc aacaactgta agtacaattc ctacgataag taatcccagg
     9001 aatctggttt tcagttttga attatattcg aatttgtttg agccgttcat gcttttgtta
     9061 tttgcttagt attaattcga aagcgttttt actaattatt taattgatgc tgttgtgtct
     9121 gatgctgctg atggtaattg ttttgcagca tattctgcct gcaaatgttt tacataagta
     9181 accactttcc agcgttcttc ttttgacaaa gcataagaat aaggctgcat agcatttctt
     9241 ccataagtta ctgaatgaaa catagtacct tctgtcattg ccatataaac cgggtcaaaa
     9301 taatttggtg gcatagcagt atattttcct tctgtaacaa taaatccttt accatcgcct
     9361 gctgcaccat gacaaacagc gcaatagata tcgtaaaaat gttttccttc tgctacaact
     9421 tctttatcgt tgatgttgaa aggatttttc acttctaatg cagccctgct ataaccctca
     9481 ggtgtattcg gtaattgata aggtaagttt aatgccatag taaggctatc agatttaact
     9541 ccggcaattg catttcctac ataaggaact gtaccatcca ctggtgtaac tgcgctcata
     9601 ccgtttggca tttcaggcat catgctatat gtttcataag cacgtgaata gttcatatcc
     9661 catgtgtaag aatcaccggc ataatcacca cctgcttttt tacaagatgc aaatacaagc
     9721 gggagtgctg ttattacaac cgctgtttta atatattttg atgcaaccat tttcatttat
     9781 tgtttatggt attataattc tttttcatga atttccactg cgccgcttgc agcaagtact
     9841 tttctgattt cttctttttt gctgtcagaa gtatgctcat ccaactcaaa tgtcattgca
     9901 aacaaatggc tcgttgtgcg ttcatcaact atttcagctt ttacaccggg tgctaatctg
     9961 tttaaataca aataagttaa aaacatacca accgacgcaa ataatacggt taactcaaac
    10021 gtgattggta tccaagcagg tagtgataaa taaggtttac caccatagtt aattggatag
    10081 ttgtttgtag ttacccataa aataaacgta agtgcagtta atgttccagt cgcaccgaaa
    10141 ataaaaccgg cggaatgcag tttggtcata cgtaaaccca tggcatcatc taatccgtga
    10201 accggaaaag gtgtataaca gtcatgaatt ttcaatcctt ctgcacgtac tttttttact
    10261 gcatcgatta agatgacttc atcgtcatat aaaccgtata ttattttttt atttgccatg
    10321 tgcaaatgct atttatactt ggttaaggtt aatgtttatg gtgatcatcg tgatgatgaa
    10381 tagcatccgg cccggtatat tggtcgcctg atgttttcaa aattgatttc acctctgcca
    10441 ctgcaattac cggtgcaacc tttgcgaaaa tgaggaacag tgtcataaag aatccgattg
    10501 ttcccaaata aataaatatt tccactatgg ttgggtaata cattgtccag cttgaaggta
    10561 taaagtcgcg gtgtgttgaa gtaacgatga taacgaaacg ttcgaaccac atacccacgt
    10621 tgatgaaaat cgacataaag aatgtgaaca caatattgcg acgtaatttt ttaatccaga
    10681 ataactgcgg agaaaccacg ttacaagtca tcatacccca ataactccac cagtaaggac
    10741 cggtacttct gttgaggaaa gcatactgct catattgatt acctgagtac cacgcagtaa
    10801 ataactcggt gatataagcc acaccaacga tagaaccggt taggataata accttgttca
    10861 tcgattcgat atgtgcgagt gtaatatatt cttgtaaatt aaatactttt cgaaccacca
    10921 acatcaaggt taacaccatt gcgaaaccgg agaagatagc accggcaacg aaatatggag
    10981 ggaagatggt agtgtgccat cccggaatta ctgaggtagc gaagtcgaag gatacgatgg
    11041 tgtgaaccga taatacgagt ggtgtagata aacctgctaa caccaatgat attgattcaa
    11101 atcgctgcca gtttttagct gttcctttcc aaccgaagct gaaaatatta tacattcttt
    11161 ttttgaatgc tgaagttgtt ctatcgcgaa ctaaagccaa atccggaatt aaacctgtat
    11221 accagaatac taatgatacc gtaaagtaag tcgaaatcgc aaatacgtcc cataaaagtg
    11281 gagagttaaa gttaacccaa acaggaccac gtgtatttgg ataagggaaa atgaagaatg
    11341 ccagccatac gcgacccatg tgaataatcg ggaataaacc cgcacaaata accgcgaaga
    11401 tggtcatcgc ctcagcagag cggtttacac cggttctcca cttctgacgg aaaagtaaaa
    11461 ggatggcaga aatcagggtt cctgcgtgac caataccaat ccaccaaacg aagttggtaa
    11521 tatcccaggc ccaaccgaca gttttgttca aaccccacat acctataccg taggtaattg
    11581 tgatgccgat cattgtaaaa ccggtgagcg ccagtaatcc tgacaatgtt acagcaatca
    11641 tccatgcctt gctaggtgcg gcttctactg gtgcacaaat atcatctgat atttgtttgt
    11701 aggttttatg accctgtatt aatggttccc ttaaaggtga tacgaacata attatttaat
    11761 tatttataat tatataaatc catgatatcc aatgcttcct cattcttatg atcagccttg
    11821 tctttatttc taactttagt tttatagaac acagaaggca cccagtggaa ttcttctaaa
    11881 attccaaatg tacgttcatc ttttgcttca ctaactacgg cgctagtatc gtcgtagctg
    11941 ttaccaaatg taatggcatc agcaggacaa gcactttgac atgctactct gaattcaccg
    12001 tctttcaatt cacggttttc ttttttcgcg ccaagttttg cttcctgaat acgctgaaca
    12061 cagaatgaac atttttcgat aacgcctctt gaacgaacgg ttacatccgg atttaacacc
    12121 atacgggtta agtcctctaa taaacctctg ttttcagttt ctttagcatc gttcatagtt
    12181 ccaaaagtat cagctccacc gtaatcgaac caattgaaac gacgcacttt ataaggacag
    12241 ttatttgcgc aataacgagt accgatacaa cggttataag ccatttggtt aaagccttca
    12301 gagctatgat tggtagcacc taccggacaa acgttttcgc aaggtgcgtt ttcgcaatgc
    12361 tgacaaagca taggttggta aacaacttcc ggattttctt ccgttcctgc atagtagcgg
    12421 tcgatacgca tccaatgcat ttcacgtgaa cggataactt catttttacc aactatagga
    12481 atattatttt ctgcgttaca agaaataaca caagctccgc aaccaacaca tgagtttaag
    12541 tcgattgcca tactccagtt aaagccattc tttttaccaa aataatcagg ataaaaagta
    12601 atgtctttcc aggcaagcgc ttcagcacgg tcttcgttac ccgcgttagg gcttttctta
    12661 tattctgcta aagttgtttc ttttacaata cggcgttgac ctaatccgtc atttaaactg
    12721 aaatgcgttt gagtttgagc cagtttaaca ttttcacctg tattagtaat agttacattg
    12781 gcagccatgt aatttacaca agctcctgat gcatctaaaa gatgatatac attaacacca
    12841 acaccggcac cacctttacc taccattgtg cggccataac ctacggcaac agaaacggta
    12901 ttgttggcct gaccaggctg aatggcaaca ggtaatttaa cttctttacc gttaacgcta
    12961 actgttacaa catcaccgtg ttcaatttta ttttcacgtg cgtaagcagg agaaacgttt
    13021 aagtaattat cccaagtaat tttagataca ggatccggta attcgtgtaa ccaagggtta
    13081 tcagcataac gaccatcacc aatagctata gattcataaa actggatttc aacaccactt
    13141 gcttttttag ccatgcccaa aatatttgca gcagcgcttg aaacatcggt tgttgccgga
    13201 gccatttccg tagcagcagt taaagtagca acaacagctt cgataacatt accgttttca
    13261 ggttgatata aaccaatttg taatgcatta ttaaaagcac ccatcgcatc tgctttacct
    13321 gctaaaggac cggtaaccca gttttgcacc atgaaatcat ggtaggtcat agcatttccg
    13381 ctccaggtta atagagaatc ctgcatttga cgggtgtcga ataaattttt gattgttggt
    13441 tgggacaatg aatataaacc tcgtttaggt tcagcatcgt tccaagcctc aaggaaatga
    13501 tgatcagggc aaatgtaggt gcaatttgca gcagtttcat tcatgctacc tgcaaatgaa
    13561 acagacattt cagctttggt cattgcttca gctaactggt cgcccattgg atgatcaaaa
    13621 acagggttac agtcatttac cattaagata tttacctgac cgccctttaa ttcgccgatt
    13681 aaagttttga tacttgcatc agaaccctgg cgtaacatat ttgcactatc ccaagtaatg
    13741 gtattgccat aattatttaa tgcgtcgttg attgcattta caaccaattg tacattttcg
    13801 tcgttgcagc cactcactac taaagattcg ccgcggtgtt ttttcaacca ttcagcagct
    13861 tcttttaatc tagcctgttt tgcttcatct ttcaatttag cgccaccagc agttgaggaa
    13921 ccggtgatag catctaataa attaagtgct acaacaccta attcagaagg agcaacagcc
    13981 acgcgataat cggcgttaga accggttaac gacattctgc tttcaaaatg atagtgacgg
    14041 ctcatttcag gtttctcttt agtaaccttt cttgttgcca catatccttt tgtaaattct
    14101 attggagaaa tccaggttcc taagaaatcg gcatcgatac tcacaattac ttttgctttt
    14161 tcgaagtgat aatcaggaat ttcagatttt ccttttgaag ccatattagc cattagcatg
    14221 ccgctatagg ataaggcatc gtaagtataa actttagtat taggatattt ctcagcgaat
    14281 gctgcgaata cttttttagt agaagggcta attacagttg aagaaacaat tctgatatta
    14341 gcatttgggt ttgccgcaag tttaccggca atggctttgt cgatatctgc ccacgatgca
    14401 ttgtcatgac cgcgtttacc ttttaccatt ggattgcgca aacgtgcagt atcgtataaa
    14461 cttaaaacac tggcctgaac gcgtgcagaa gttccacctt tagtcacttt tgaatcgcgg
    14521 ttaccttcaa ttttaatcgg gcgaccctcg cgtgttttaa caattatact acagtagtcg
    14581 ccaccattat aaaatgtaga tgcataccag gtagcaatac cgggaattac atcttcaggt
    14641 ttaaaaatgt aaggcgcaac cttttttaca ggaatttcgc aaccggcagc aacagtagct
    14701 gcagttaggc taaatcccat cattttaagg aaatcacgtc ggtttgaatg caagccatcg
    14761 ttgctgctgt caaaaaactc aacaggaatt tcctcgccaa actcgggttg tatgagttga
    14821 gctgattccg gtgcctgatt taattcttca aggcctttcc agtatttttt ctctgccatt
    14881 tggttatggt tagaagttta agtgatgctt tgttaaaaaa ttaatagtga catttttgac
    14941 attctgttgc accgatgtct tcagctttta ctgattccat ctcaccattt ttaatttttt
    15001 catgcaacac ttcgtatggt ttataaaact cgttagtagt aaagttgaca tttgtattgc
    15061 ggtgacaatt cacgcaccat cccattgaaa ggttttccat ttgttgaact acgtccattt
    15121 cctggatttg accatgacaa gtttggcatt ccaatccggc aacttttacg tgttgtgagt
    15181 gattgaaata aacgtggtca ggtaaattgt gaattttagt ccattcgatt ggtttaggtt
    15241 cactttcata tttcatagat tcagtattga atccgatata gctataaatt ttagcaattt
    15301 ccttatcata aactgcctta ggatagttag ggtcggttcc tgtaacaccc tgaatgttat
    15361 agtgacagtt catacacacg cttaatgaag ggataaccgc agctttaccg cgttcagcgc
    15421 ctacgtgaca gtatttacaa tcaattttat tgataccagc gtgtacttta tgtgaaaatg
    15481 caattggttg ttcaggagcg taaccttgct gacgaccaag tgaagtagca ctgttccaca
    15541 tggcaacact taataatgat aaaaacaata aagctaaaaa gccaacaagc gggcggtatt
    15601 tacgttgctt gtaaaaaggt gttgtatcag gtgtagggtc atcaaatttt tgacgaacta
    15661 atccgtcaag gtatttaaca gcgcgtccaa gtattaccgc tataaataat aatgcaacaa
    15721 ttaaaatcca gtataatgta gaattatcct tttttgtttc ggttacaaca ccgcctgggc
    15781 cgggtgccgc agctaattcc tgtttgcgtt ttgcttctgc atcaatataa acgagcatat
    15841 cagcaatgtc agcatccgac aattcaggga atgccggcat gatgctgtta tattctttat
    15901 ataagtcgtt agcatattta tcgccggtgc ctaaaaaact ttgtgagttt tttatccaag
    15961 cgtataaatt agtgctatca gcccaacgtg tacgcacatt ttgcaatgcg ggtccggtca
    16021 attttttgtc cactaagtga catctttgac atttggattg aaaaagtact ttcccttcag
    16081 cttcacccgc aaatacgtta tttgctgtaa atgacaatgc gagtagtaaa ataatgctca
    16141 ggaaacgtgt gatggtcgtc gacttcttat gctgcacaat ccttgatttt attggttaag
    16201 gtatttcggg ttttataaac ccgtgcaaaa ctaaacacca aacatgaaaa gtttaacaaa
    16261 tcttttaaaa aaattttcaa tagatgcaac catactcaaa ctcaattggg agtaatgttt
    16321 catcgcataa taattcgctg tttagacgca ttctaaatta aaaatcatat ttattcacat
    16381 ggttatcaca cttttatatc cgtttcgatg cattaactgg catagttttg tatttataaa
    16441 agattaaaca ggaggaaatt atgaaaaaat tggtttacgc ttcaatgtta ttattagtga
    16501 ttagttccgg atgtaaggta gccgaaaaag aatttaggga aggcaattat gatgaagcca
    16561 taaacatctg tataaaaaaa ctaatcgaca accctgataa ggttgagtat attttgttat
    16621 tagaggaagc gtttgtgaga gcaaatggtg aggatctcga ctatataaaa gccttaaaca
    16681 cagacggtag tccagaccgc tgggaggaaa tttataacgt ttacgagaaa atatatggcc
    16741 gtcagctcaa aataaaaaga ttgctaccgt tgtacgtagg aactgaagag cgcgacgccg
    16801 aattcaaatt tgccgatgct ttagacggga tgaatacagc taaaaagaac gcagctgcct
    16861 attggtatgc tgatgccagt ctcaaattga aaagtaacga cgtttacaaa gcacgtgaag
    16921 cttattatga acttcaaaaa attgaacgtt tttacaatga ttacaaagac gctgacgatt
    16981 taatgaaaaa agccaaagaa attggcacta acgatgttgt atttaaagta gaaaacatgg
    17041 cgaatgccag cttaaactac gatgttacaa aggctatcaa ccaaattaac ataccaaaat
    17101 caagtggcag ctggtatatt gtacatagca gtgtatttga aaaaggtgtt gagatgacca
    17161 ttcagttaaa tattcgcaag attgaagctt accctgaaaa agtgagtacc aaccagtatg
    17221 aggagtcaaa acaaattatt gacggatacg aatacgttta cgacaatcag ggcaatattg
    17281 taaaagacag tttaggtaat ccaatcaaag aaccggatta taaaaccgtt actgcttatg
    17341 tatctgaaac ctggcaggaa aaagtagcaa ctgtaagtgg agaggttgtt tatttgaaca
    17401 aaaacggtga agtgatacgt tcgtttcctg ttcgtgcaga tgctgttttc aaaaactatt
    17461 atgctgcagc taccggttat gccgaagcgc ttactccgca gtcgaaggaa aaattaggtg
    17521 gtaaaccact accctttcca tacgatgacc agatgttgat agatgcagtg aatctgttag
    17581 aaaatgaggt gaggtctata accaatgcca acaacgacga atatttgaac ttataagata
    17641 aaaccggtta acagccggtt ttttttgtca cagaattgta tcattatccc attttaataa
    17701 atcttaaccg aagcagtttt gccaaaatct tacctttgac ctatgaatat caagcgcttt
    17761 gcacaattta gttgccttat attttttatc gtattgtccg gcaacaattt atttgcacaa
    17821 tcctatacta ttagtggtta tgtaacagaa aaaggttctg gtgaaaagtt attaagcgca
    17881 accgttttta acagcaaaaa ttttagtggg acttcaacca acgattacgg tttttacagt
    17941 ttaaccttac cgggagattc agtaataatt aattactcct atattggtta cattacacaa
    18001 caaaaaaagt ttgtattaaa aaaagatacc gtgcttaata tcgcattaga gcaatccaat
    18061 gaattaaaag aagtggttat tgaaggtaca aaaagtgatg agcaatttca ggaaaagaca
    18121 caaatgagca ccattgaaat tcccattgaa caaattaaag cattgcctgc atttttagga
    18181 gaagtagatg ttttaaaagc gttacaatta ttaccgggtg tgaatggtgg atcagaaggt
    18241 tcaagtggtt tatttgtccg cggcggttca cctgatcaga atttaatttt attagatggt
    18301 gtgccggttt acaatgcatc gcatttattt ggatttttca gtgtgtttaa ttctgatgca
    18361 attaataaag tgacattggt taaaggagga tttcctgcgc gatatggagg aaggttatcg
    18421 agtgttgttg atattagaat gaaagaaggt aatatgaata gttatcacgg agaaggttct
    18481 attggcgtta ttgcatcaag gttaactata gaaggaccaa ttattaagga taaaacatct
    18541 ttcattgtgt caggtagaag gacctatctg gacttaatta ccaggcccat tatgaaatcg
    18601 cagcaggatg aagaaggtgt atcgggctat tatttttatg atttaaatgc aaaaattaat
    18661 cataaatttt cagataaaga ccgtttgtat gtaagcggtt attttggtcg cgacaaagct
    18721 tattttaatt acgattatca atatgatgaa accactcaac gtgttgaaga taatggaatt
    18781 tattggggga atgcaactgc tgtagcaaga tggaatcaca tgttcagcaa taaaattttc
    18841 agcaatttaa caacaacatt tacagattat aaatttgaca tatatagtac cgtaacagaa
    18901 ataaaaactg acgacccgga tacctatttt gggtttgatt atttttcggg catttacgat
    18961 tggagtttaa agtacgatat agattatgtg ccaaatccaa aacattatat taaattcgga
    19021 gcaggtgtta cctatcatac gtttgaaccg ggagcaacac aatttgaatc ggaaaacatt
    19081 caggaatttg aagatgcttt ttcgtttggt tcagaaaaag tatatgccag tgagtttgat
    19141 gtttatatcg aagatgattg ggaagtaacc aataaattaa aaattaacgg cggattacat
    19201 gccagtggtt ttttagtaaa tggcctattt tataaatcgt tacaaccacg aataagttca
    19261 aggtatttag taaatccaaa tctttcaatt aaggcttcat ttagtaccat gacgcagtat
    19321 attcacctgc taaccaattc cggaatagga ttgccaacag acttatgggt acctgctacc
    19381 gatagcgttc cgcctcagtc agcaattcaa ccagctttgg gtgttgctta cacattaaac
    19441 aatcaatatg aattcagtat tgaagggtat tacaaaaaaa tgaatgacat tattgaatat
    19501 aaagatggtg ctaattattt tttcgatggg cttgaaaact gggaagataa agtagaaagt
    19561 ggtgaagcct gggcctatgg tgcagagttt tttattcaaa aacgtacagg taaattaagt
    19621 ggctggattg gatatacgtt atcatggagc aaccggcaat tcgctacaat taatctaggc
    19681 gaaaaattcc cgtataaata tgaccgcaga cacgattttg aaattgcagg aatttataaa
    19741 atcaatgacc atattgaact gtccgctacc tggatttata catcgggaca accggtgagt
    19801 ttgccaattg caaaatacga aggtgccggt ggtcaaacat tatatgctta cgaaggaaga
    19861 aatggttaca ggatgaatgc ctatcaccga ttagatgcca atgttgcatt gtcgaaagaa
    19921 aaaaagtatt ggacaagaac ctggaatttt ggtgtatata atgcctatag ccgactaaat
    19981 ccatttttca tttatcaggc atatgattac aaaacaaata ctaacaacta tcgacaagtt
    20041 agtttatttc caatcattcc gagtgtatct tacgaattta ctttttaatt atagattgca
    20101 ttaaatatag cgcatgaaaa ataatatcat aacaatagtt gccatttcag gtttggtgtt
    20161 tttagccgct tgtgaaaaaa cggtttactt tgatgtagaa acaaaagaaa atcgtttagt
    20221 agtgaattca tttttacaac ctgatagcac taatcgcgca gtagtttcgt tgagtgttga
    20281 cccattggca attggatttg aaagtgagcg agtgagtgat gccaccatcg ccataagtaa
    20341 aaacggttca tttatcggca attttacgca tgaatttaat ggcgattatt atattgaaaa
    20401 cgatttattg catgctgagc caaatgatga atttgaatta acggtaagtg ctccgggaaa
    20461 agaaaccgta actgcaagca ctacaatacc atcgctaatc cctataactt cagcagctat
    20521 catagatacc tttttaattg aaatttctta tagcagtatt gacccctatg gcaatgtaat
    20581 ttatattgat acattagttc cctatttcaa aatggcaatt acttttaccg atccgggaac
    20641 tgaggattat tactctctag cggtaatgta taaggatatc ttcaacgaat catttgcctg
    20701 tgtaagtact aatgatccaa tatttacagt tagtggtgaa tataattttg gtgcagaaaa
    20761 tcaggcgggt gctgctacat tttgtgatga agtaatgttt actgatgtta cctttaacgg
    20821 aacaaccaaa actgttgaat taagtattta tgccattaac actataggca tagccgatcc
    20881 tcgatttgaa ttttcgttaa aacacgtgag taaagaatat ttccaatatt atacgacaac
    20941 agaaaagcaa ttagaaaatg aaggtaaccc atttggagaa cctgtaatta tttattcgaa
    21001 tattgaaaac ggttttggca tatttgccgg tagcacattg tctaaagcaa taatagagtt
    21061 ataagtgcat gttgaacccc aacaaacaaa gcggcttgct gtttgatgga atggcaatac
    21121 ttaccgggct tatcatgttt acccgggtaa aggaatactt ttacaacaat tatacagcta
    21181 ttggtccatt accttgggaa attacttgtt tcataacaat aggtgtaatt tgttatacgc
    21241 ttggcttgtg gttttataaa aatcacttcc tcaaaaacat tagtgaaacc aaggaaaaaa
    21301 aacacggagt tgtacaccaa atcagtttat tagtattgac gcttatgcat tttgcaatat
    21361 tctgtgtatt ggcagttgta atagcagata gttggcaggt ggtatttccg agtaatttct
    21421 tacggtttgt tttttatggc ttaattggag gcttgccaaa tttgatatta atatggactt
    21481 tttatcgata tcaaaccaac caatcaaact atgaaataaa caatatcaat attaccattg
    21541 gcaatattct attaagtatc agtgcgattt ttatacaagt attattttgg aatgtaatga
    21601 caattccaat gttacaaaat ttacaagtcg aatcgtttta tggacgagtg atatttgcag
    21661 gattattttt ctttttattt cttttaattt atctccctcc caggttattt tattttatag
    21721 tagattatcc atacacccgc acatggatgc aaataacact tgcgtatttg ccaacaggtt
    21781 tatatatcat taccaagtga aatattatcg gaacaacctt gctaaaattg ataccttatt
    21841 taattaaaaa tactattttt attaaaaaat cgttatcctt tcatcttaaa ataaacaact
    21901 atgaaaaaaa attatgtatt agcaacatgt ttgtttttaa ttgctccttt gctgcatgga
    21961 caagttgctg cagacgaaat taaaaaaaca ccttatatag aagttaccgg cagtgccgaa
    22021 atggaaattg ttcccgatga aatttatatt caggtcacat tgcaggagcg gtataatggg
    22081 aaagaaaaaa ttaccatgga gaaacaggat gcactattta taaccggaat gcaatcgctc
    22141 aatatcgatt cgaaggatat tagtttagct gatgcgcaat ctgacttctc ttcttataaa
    22201 tggaaaaagg aggatgcact tgctcgtaaa agttatgttg ttttagtgca tgatgccgca
    22261 ttggtatcga aagtttatgc taaattggta gaaatagatg ctgaagatgc gtatatccta
    22321 aaaacaagtc atagtaaaat agaagaatac cgcaagcaga taaaaataaa tgcgatgatt
    22381 gcaaccaaag acaaagcagg ttatttgttg agtagtatag gtgaagaagt tggcagccca
    22441 attttaattc aggagcgtga aacttatgat tcaccataca caataagtca gcgaagttta
    22501 gcttccaatg tcgcctttga agcgtataac ttaaatgcaa gtgatatagg ttttaaaaaa
    22561 atcaaactgc gctatgaaat tttcgcgcag tttgagataa aataaaaaca atgccggtaa
    22621 ttcagtttac cggcattatt ttgttattgt ttaataaatt tttgtgacgc aattaactca
    22681 ccttttttat tagttgctaa agcagtgtat attcctgcag ggaattcatt tacattaaaa
    22741 ggcgtattac ttgttgtgtt gataacggca atttgttgac cattgctatt aaaaaatgtg
    22801 atcgtaaaat tgtcattata attttccatt tccattacaa ttgttgaagt tgccggatta
    22861 ggtgtaataa tggcattagt cagattgttc caattttgga tggcaacctc aggtaaggta
    22921 tatggtgttt ccattttgac aataaaaatg tctatttcct ctgatacaga tgcaattggg
    22981 tcgataccat caaaattcaa attcccaaaa aagtatccgg acaaaagcaa gttattattc
    23041 tcatcaatag caaggctttg ataagcttct atattatcgc catccgtttg agatactaca
    23101 ttaactgttc caccttcaga aatttccgct aaataaccat ctgcttcaac ctcagcatat
    23161 aattgtaatc cctggaattc tgctgcgcca taaaaatacc cgcatacata aaaattattg
    23221 ttatgacctt ttatcatttt attcatggga tctgaatccg ttccaccgcc atgcaaaatc
    23281 caggtaacat ttccggtgtt ggctgccagt ttcatcataa ccatatcggc ttcaccatat
    23341 gaatttattg aacctccgat ataatttaac gtctcaccca ttgttgtggc gtaatatata
    23401 ttgcctgctt cgtcagtttc taaatcagca aataaatacc catcacctgc agcactaccc
    23461 atattttgat tccattcgat aaaaccgttt gcatctgttt ttaccacaaa aatgtcgctg
    23521 ctaccataac tatcgaaaaa ataactatcc tcttcgaaca taccttcatg aaagccggaa
    23581 ataattatgt tgccatcaac atccactgta atttcatttg caaaatcata atcagcacca
    23641 ccgatagact tcatccaaag tggatttaaa tcagcttcca ttttaccata aatggcatct
    23701 gaatcaccac tgtggtcaac ttcggttaca ccaaaatcaa aaaaaccttc agcagcgccg
    23761 gcaataaaaa tatttgcctc ctcatcttgt gataaggcat aaaaataaac ggattgtgtt
    23821 tcatcagatg cataagcagc agtgcactca ccgtcggcat tatatttagc gacaaagccg
    23881 cggtaattat tatcactttc gttgccaaaa ataaaatcgc ctaaccaggc ttttccaata
    23941 aaaataccac agataagaat accaccctcg gcaagtggtt ccatatcgag cactacgagg
    24001 tattcagtcg cgtatactaa ctcgagaaac ttgggagtgc catttttatc atgtttagta
    24061 atccaacccg ctgcatttgg ggttccgttg gtaagttcaa acccatccag actaattgta
    24121 ctgtaaaaca gacctccggc ataaacattt ccttcgttgt ctacagtgga ggcatacaat
    24181 tctgcttctg caaaacttgt cgcccaaata gcttgctggg cttcgatttg gcttatctga
    24241 atcaccaaaa tcgcgataca taatacagtt aattttttca ttgtttttag ttttataaca
    24301 gcaaaagtag acctgccata aaaggtaaat ggatgaaaaa agggggtaaa attagatttc
    24361 gaacgcttta aaaataatgt atctaattga ttgtcaatta gttagagtta aaatatcgta
    24421 ttatttattg gtgcattatc atttaaagat ggattttaaa cccatattga ttgccttcac
    24481 tttgtatctg taaaatataa atactggttt gaggcgggat tgtaattgtt gaattgccta
    24541 tttgttggtt cgaagtaaac actattttgc ctgataaatc aaatagttgt gtggtatatg
    24601 gtaattgatt tggtgttata atctgaattg tattattacc aaagttcacg atagagaaag
    24661 gtgaagaacc ggattcaatt tcagcaacac tattaaatgt tgctcctgta cgtattggtt
    24721 tatttaatgt agtttcatag gcgaaatcat gtataatcat cgacaagggt tcttcgataa
    24781 tagttactct gatccatcca tagtgtgctt cacctaatac acccattctg attcctatat
    24841 atgcttcacc aacagcactc cagtgtccta cattttttat caaattgtgt gactcatcaa
    24901 atacatgtga taccattagt ggccaacctt ctgattgaaa actgagcaat ggtccaacag
    24961 aataatcgac aggaattgca taagggcgaa acgtttgata ggaactatac atagcacttc
    25021 caacagtaaa ataacttccg gcaaaaaatt tattttcacc tagattatcg aaaaacaatg
    25081 ccgtaaaact tctataaaca ctttcccaat aggaataata tacgccatta attcttagca
    25141 attgaaaatc atttacaccg ccattatcaa tatctatttc ataataataa tcttcattat
    25201 ttaaagtaat atccggctca atatcggtat acacaatttc cgcatttaaa ttatgtgtaa
    25261 acagaaatgc agttgcaaat gcagcataat tatttaattt ggattgtaat tgtgtgttat
    25321 ttatcataaa ataataaatt gatgtgattg cgtttttcca ttcgccgaaa caacaaatat
    25381 atacatccct tttaaatcgt tagcataact taaattggtt tgattggtat catcaaattg
    25441 gtaaattaat tgccccaatg cgttataaat agcagcgctt tcgtatgcaa atgggataaa
    25501 aacatgtatc tcatctgtgg ctggatttgg ataaatagta aaatcaaatt catcactaaa
    25561 tatttgttcc ggcaataatg cagtttcgct ccataaagtg caaccatttg cctgttttac
    25621 actaaccata taagtaccat tttgtaatgg cgtgtaatat tgactattag cccccttgat
    25681 tggcaaacca tttaagtacc attgccaata tacaccttca actgcagata aaacaagtcc
    25741 ttcaatggct atcggaaaca attgagtagt gcaggtgttc gatcgtgcaa caaaataatc
    25801 agcttgatta gaaagttgaa tattaattaa tgtatccgta ttaaaaatta aagtgtcttc
    25861 agaaaaagca ccaataagta acatattacc gaatatgtcc cactcaactt ttggaatatc
    25921 attgtctcca tccaacggta ttacaaattg ggaaatcggc aattgagctg tatcataaac
    25981 taaaacagct aaatcagcac cgctgtttgt ttcattaaaa atagtatcca cgccggtcca
    26041 caaagaatta taaacctgtg cggaaacata aaataaatta tcaggcgata cattgatggt
    26101 tgcatatcca ggaaaaaaac tactcgattc agtggctgaa attaattcac cgtttaagtt
    26161 gaatttagcc atgaatgtgg tgttaatttc ctcaccgtga acaatggtat cactatcaaa
    26221 aattgccaat tcatcgttca gccagccaac taacataatt ccatttggaa ttgttacagc
    26281 atcctttaca taaactgatt gcgggcttaa taatcgggcc cagctgaagt ttccatatac
    26341 atcgcatttc cctaaaaaat ttgatgcatg ataacttgaa tcaattaaca agaaggtatc
    26401 caccttaaaa gttgcacctg aaaattcgcc ataaaatatc aaatccccat ttgtagtttc
    26461 aacgcctagt aaatcatcac aatcacctcg aaatacttct gcccaatcga caatattggc
    26521 tgatgtataa tgggcaacaa acatcctatt tccatttaca tttgcatcca attgaatttc
    26581 tcccaaatct actccttcgt tgtaataata ttgatgtgcg gttacatacc aaccaccaaa
    26641 taaatcgctc actaaagaag ttggaatcgc gcctttttca aatacatggc tatacatgac
    26701 ttcacctgta ggtgataatt ttattataaa tgagaaatat ccttcctcta tactttccag
    26761 atataagtca tccaaaatta tattttcact ataacttgta cctgtcagaa taatatttcc
    26821 aactgcatcg aatattacat catttatttt tacatatgta ctatattcac tttccgcgta
    26881 caattgtttt gcccaaattg tttttcctga actgtcaata attgccaaat aactgccagt
    26941 cgaggaaaca ttggaattgc ttatgactaa tgtatcgcct atttgaagcg cggggctata
    27001 aaatggaccg gcaaggagca tattgccctt gttatctaca tcagtaaatt gtatggcatc
    27061 gttacttgta ccatgaatgt atgttgccca agaccaatcg ggtgcttgtg ctgaaaggtt
    27121 taaatgaaat aataacaaat aaaaaaaaat ggaccaggca ctaattttag ccttaagaaa
    27181 attttggtag caatgatata acgaatgtgg ggacattggt ttcatagcgg gaatttattc
    27241 attcaaatat acgaaaaaac cccagtaatt accggggtta aaaaaatatt gtcaaaacta
    27301 gttaagccaa ctcaacacct tcaaccaaaa cactcacatt attattgagt atttcaacga
    27361 agccgccttt gatgttgtaa accacatttt ctttgcctgt gtttacgcgc attttgcctg
    27421 tagtaagtgc tgcaatcata ggtgcgtgat tgtttaatac ttcaaaagag ccggtaacac
    27481 caggcatttg aacgccaaca gcttctccgc tgtagatttt cttatccggt gaaagtattt
    27541 cgagttgcat tgatattatt cttaattctg gttaattgta ttatttagct tcagcaatca
    27601 aacgtttacc tttttcgatt gcatcatcga tagtaccaac aaggttgaat gctgcttcag
    27661 gatattcatc cacttcacca tccataatca tattgaaacc acgaatagtt tcttcgatag
    27721 gtactaacac accttttaaa cctgtaaact gttctgcaac gtggaatggt tgagaaagga
    27781 aacgttgaac acgacgagcg cggcttacaa caagtttatc atcttcactt aactcttcca
    27841 taccgaggat ggcgatgata tcttgtaatt ctttataacg ttgtaaaata cttttcacac
    27901 gctgagcaca attatagtgt tcttcaccaa cgatatcagc tgaaaggata cgtgatgttg
    27961 aatccagagg gtcaaccgca gggtaaatac ctaactcaga aattttacgg tttaataccg
    28021 ttgttgcatc taagtgagag aaggtagttg ccggagcagg gtcagttaag tcatccgcag
    28081 gaacgtaaac ggcctgcact g
//
LOCUS       DAORKW010000003        27546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5_length_27546_cov_6.168673, whole genome shotgun sequence.
ACCESSION   DAORKW010000003 DAORKW010000000
VERSION     DAORKW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 27546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27546
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5_length_27546_cov_6.168673"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /locus_tag="PLB46_00270"
     CDS             <1..1846
                     /locus_tag="PLB46_00270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF74972.1"
                     /translation="LSELGLAISENEFSNLIYGPDPHPVIKNYYVGLSQTGQYDPSVL
                     PGFVENISNPETQQNNPQARQEYYQFVSREQVAKRDYKQTKYMSLFTKSAYVPEWLAK
                     RSYTVSNTRGTFTMISLPYTQIADSTITTTDADLKKYYNENKNKFKQTEGRIVEYVSW
                     EFLPTAKDSAETLKALNENLVKMAASKSDSAYMIARSDDPDKFGNSYYSRNDLYAQGI
                     DSSVVSNFYSQAVGALVGPFENGGMYRVAKIKNRSAMPDSVKARLIVVGITPERDSIK
                     AKGLADSLLAVLSAGGDFAALAAQYSDDQQSGQQGGDIGWATPSMNYIPEFKKYFFET
                     GTTGKTSIIKTANGYQIIEITEMSDRKELVNVAFMSKTISAGKETIDSIEKAATAFYE
                     KYQTPEDFEQGVIDNRLFKRVTQPLAKNMYEVPGIENTREMITWAFDAAKDEFKFFNM
                     SERVIVAYVKEVRVNGIADLENVREIVEQEVIKEKKAEQLGKQIKDAMSAGSMDAVAA
                     KLGVRVDTVRNASFGSPNAPILGREPKVIGTVFSTELGKTSNVVAGVRGVYVVSPIEF
                     TPVPETSDYTINKNQLTYTMQNKYQGQTLLNELKEKAKVTDNRYLYGE"
     gene            1994..2497
                     /locus_tag="PLB46_00275"
     CDS             1994..2497
                     /locus_tag="PLB46_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HRF74973.1"
                     /translation="MELVNKVAQSGIISFDLETYFPDEKRIVSFDLKDYLFKGLILRE
                     KDFREALAAINWEDYTGKYMAIFCSADAIIPNWAWMLIVAQAEPFAAKIVMGTPDDLI
                     KQMYRATLATIDIETFRDQKIIIKGCSDKPVPADAYLEITKILRPVAKSIMYGEPCST
                     VPVFKKK"
     gene            2503..3471
                     /locus_tag="PLB46_00280"
     CDS             2503..3471
                     /locus_tag="PLB46_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRF74974.1"
                     /translation="MKKIHYLLIGAFVGVAIFIGYAVSGNDKTTNNENIAVATDTLRA
                     LADDVTTIRYFAAKMPESTTFAGEPVPLNDIEVRERLDRELTVNGYWHSNMIQNLKLA
                     SRWFPVIEPILKENGIPDDFKYLAIAESGLRNVISPSDAHGYWQFLKGTGLQYGLTIN
                     AEVDERYDMEKSTLAATKYLKDAYAKFGNWTLAGASYNAGMGAIEGAVNYQKEDAYYD
                     LYLKDETSRYIFRALAYKIIFENTQKYGFFLTKDDLYNPLQYQTVKVDSTITDLADFA
                     QKHNTNFKMLKYYNPWLRSTLLTVKKGETYQIRLPLTATVKTGGTQ"
     gene            3521..4240
                     /locus_tag="PLB46_00285"
     CDS             3521..4240
                     /locus_tag="PLB46_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF74975.1"
                     /translation="MTVKKLSVIVPAYNEEATIATILTTLSNVQLIHDIEMEIIVVND
                     CSRDNTAANLAAFMAAHPQVNIRAFHHEKNKGKGAALHTGIANATGDFTVVQDADLEY
                     DPWEFNILLKPIVERNADVVYGSRFMGGKPHRILFFWHSIGNKFLTRLSNMFSNLNLT
                     DMETCYKLVRTSILQSITLKENRFGFEPELTQKLAKVKNIAIYEVGISYYGRTYAEGK
                     KINWKDGVRAIYCIIRYGLFK"
     gene            4243..4962
                     /locus_tag="PLB46_00290"
     CDS             4243..4962
                     /locus_tag="PLB46_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF74976.1"
                     /translation="MNLPKLDIVLPCYNPANNWVNAIIESYQALSTLLPQTAIKIILV
                     NDGSTKQIAESDLTQLQQQIVAFQYIHYPVNRGKGYALRKGVAATNAPICIYTDVDFP
                     YTTNSIMDLLQQLTSAQFDIAAGVKNKNYYKHVPPFRRFISKLLRACTGFVLRLKVAD
                     TQCGLKGFNEKGKELFLSTTIDRYLFDLEFIFLASKNKEVTITPVPIELNENVHFSSM
                     RLGILFTEAKNFITIFFKNFK"
     gene            4962..7265
                     /locus_tag="PLB46_00295"
     CDS             4962..7265
                     /locus_tag="PLB46_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74977.1"
                     /translation="MHILRKHPLVVLIVMSIIIICGVWSKIIFHPNTYLLSSGGDASK
                     NNFTPAWFVKYDEGTRFTGMHYPYGEHVVYTDNQPAISWALNLIDNNIYPIHNYTVGI
                     LNILLFVSILFGIIFLFRILTYYRLPGWYAAIMALAIGLFTPQLDRFWGHYALGYAVF
                     IPWLWWRLIKVKENNYKLITIFFTILLITFFGFIHMYYVLIGALMVGFHACVVFIKNK
                     RQYMQAIKLLAIAGIPLLVVMLFMFFTDPVNDRPETPYGFFRYKANFQTVFLPKHGTL
                     TNQAKKLFHFGHADAEGYAFVGYTGFIFLFIILFIYFKRLWFKRSVRNALLPMPGDLG
                     AFFITGICMLLFSMTIPFNLGLEFLLDILTPLKQFRSPGRFAWVFFYIYLVGIAVLIY
                     VIHKKIQKRYPKLAIAFLGAFTILLLVEGLSWFSLTAKGIQTNNESNPYDGKNTFFAD
                     MFNGTAYSPDDFDGILFLPSIFQGSEKLYIDRTGWGNFPKAMELSYQTGLPLVSYMMS
                     RTSQSETFNDVLIAAHPYIKVESTEGVFSLKKLVIMTTDRPKTIGENYILNHATKFSS
                     KAGFDLYAFDMASIKEKGPALAINDYLQVKDSLQVFTSPTGTVYQAEKPLEVLYYDSF
                     DGQASERVFLGKGALNNKREAVPVADFAVSISDTIWLEASCWATNDISEVAYPYLAIY
                     YYDEAGNMVKELGISPTQTCDVMGNWVRASDNFEIYPSIKRIKVVSTDNSQAWFDELL
                     IRHSAYNAYYHVVSDSNFVVNNFKIGK"
     gene            7360..7432
                     /locus_tag="PLB46_00300"
     tRNA            7360..7432
                     /locus_tag="PLB46_00300"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7393..7395,aa:Phe,seq:gaa)
     gene            7536..7805
                     /locus_tag="PLB46_00305"
     CDS             7536..7805
                     /locus_tag="PLB46_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF74978.1"
                     /translation="MEVICLEDEAFYALVESVVRRIKEQQGIKEDKWISTEEAMNKLR
                     IKSKTTLQKLRDEGQIRFTQPEKKIILYDTDSINEYLEKHSKDIY"
     gene            7805..8038
                     /locus_tag="PLB46_00310"
     CDS             7805..8038
                     /locus_tag="PLB46_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74979.1"
                     /translation="MEFNEKHFILGFNSGYILANHEPIVLSSVIKELQSDNSYIKGLL
                     QGQIEFENEQSKFRLDELRQSRGKSIDTKEKDL"
     gene            8416..9381
                     /locus_tag="PLB46_00315"
     CDS             8416..9381
                     /locus_tag="PLB46_00315"
                     /inference="COORDINATES: protein motif:HMM:NF019371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HRF74980.1"
                     /translation="MEKKPFTKPHLTFHEQLEMLKSRGLMIQNEGETLSFLRGVNYFR
                     IIPYCRLFEYRIEENPDEHFFKPETSFEKIQRHYLFDRELKLLVFDLIEEFEIGFRTQ
                     FSHEYSKIENPDAYFDDSGNFIRKWWYEDLSLYRKYKLYQKDFKTINFEVDRAKNDPF
                     IMHYWNKYEIPATLPSWMMFEVISMGTLSRLFSNLKITEPKKNLTNFYKLPSPHILEN
                     WLYNIVFVRNICAHHGRLWNKSLKTVVFPLKSERCNWLLNTEISATKLYSLLSALLYL
                     QSSISKTSVFITRFKDLLQRFPEIDTEAMGFPLNWDQEPLWNQIT"
     gene            complement(9500..10663)
                     /locus_tag="PLB46_00320"
     CDS             complement(9500..10663)
                     /locus_tag="PLB46_00320"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HRF74981.1"
                     /translation="MSTDDYDITPIGYTTWRNQNIPFGIKDKDRLGHIYCIGKTGMGK
                     STLLQKMALSDITKGKGCGVIDPHGDVAKYLLERIPEERKKDVVYINPVDENVIAFNP
                     LHAVHPNYHSLVASGLLSTFRKIWSESWGPRMEYILRFSLLTLLQYPYATLLDIQPLL
                     TDKHFKEKVLEYVKDDYIFDFWRKEFDTYTPSFRNEVISPILNKMGVFISSKPLRNML
                     GKQVSGFRMSQVMDEQKILIANLSKGELGEDASTIIGSILVTSIQLSALYRAKQPMDK
                     RTPFYLYVDEAHSFLSLSFADILAESRKYGLSLFLAHQYIEQINEEIRSAIFGNVGSI
                     ICFRLGANDANLMAKEISNEIEPLDLLRLGKTEIYLKIQIDGTSENFFSARTV"
     gene            10942..12612
                     /locus_tag="PLB46_00325"
     CDS             10942..12612
                     /locus_tag="PLB46_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRF74982.1"
                     /translation="MPPYPQNTTKPQAVIYCRVSTKQQVDEGNSLSTQERLCREYALK
                     QGYEVAAVYIEKGESAKTKDRTELQKMMSFCAIKKNNISYVVVYKLDRFSRNTDDYSY
                     LRIILKRYGIFIKSTTEYFENTPAGRFMENIISNVSQFDNDVRTERAVNGSRDAMVEG
                     RYVWSAPYGYSNIKVGGRGNIAPNDNAPIVRRAFEEVAKNQYPIEEIRKSMFHSGLTT
                     YMNKPLSRAQFYRMLKNVAYSGWIVKFGERHKGIYEPIVSDELFEQVQRVLNYRTRKN
                     FTYNCKHPDFPLRRFVTHPNGMKLTGSWNQGKSQKYPYYRYMCRMMNFPKESLESQFM
                     AFMDTFKLEGGQYDALKKKLKGALEKGVKYRLKDRDKVLQEVKELKLKQSLLIEKNIQ
                     GVISNELLRDQLVLIDKQLLELNSKLYIEPTYEVNLEELITKAKTLLTNPSEVWKTAN
                     NDNKLRLQVFEFPHGVTFDGEIFQTPEICRLFKAKDLFLPSSSHNAGAKKKVIKFHPE
                     VANLPYITGDKPSPCATYNPTTKKYWEEIVKDLIVVNNILSEELIDDT"
     gene            complement(12753..13040)
                     /locus_tag="PLB46_00330"
     CDS             complement(12753..13040)
                     /locus_tag="PLB46_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74983.1"
                     /translation="MKTRRKKTPLEIWETGKRNAGRPRKITPEEDWLLDELVDILVDV
                     YLDEVKRVPSIVSDIVPQAELHTRHKVETSLEHLDTYARAPILRDGLHNRV"
     gene            complement(13049..15007)
                     /locus_tag="PLB46_00335"
     CDS             complement(13049..15007)
                     /locus_tag="PLB46_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF74984.1"
                     /translation="MYLNTLSIFNYKSCQGISIDFFKDDPTTIIGINDSGKSAIIKSI
                     GLLLDPKTPFNISGDTRATSDISNTLLGSEQCAEIFKKLNILPFSDQINGSAILGEFI
                     IEDEEYTDEFKESLSEQLRWSLESFANKKIYLLKAYEPGDSTGKYFLYTNDAEKPLGL
                     WNKKATELTQARKELGVTDEDIRNDNSKGRFGNLEIIRGIYSKTTLKPVWSEYPSFIK
                     ADLPKFPVYRYIDWNTSLADIENLANDVMKKHIEDSKKALADQAADVSKNATEEVNKE
                     FESLTKELTRDLNNISAIKAKVTFTVAEKVSDIIINKTTSDGDIRLDSQGEGVKRQIL
                     FAFLKWANTRNSEETETSHKQFIWCFDEPESHLYPTAQRDLFAILAGLSQGNYQIILG
                     THSTIFIDRLRLNEIYKIKLKEKYSEILKCTTIDDVHDVLGVRNSDILFYDLFLAVEG
                     ETDIVLLPHFYKLHSGKSLEENAIKLIPLNGSGNYKHNKQILENILGDFKKVESTVHY
                     VFDSDTGETGANVYLLGTCDIEDLFPNEVWIRLVQEKCGIGVTDDELNGLRGQLDPKI
                     ENRKFHKLLAALVANKSGQTVYLPSKRICAQIIQKYIITIEQVPNAIINVLEKIKNVQ
                     KLDPPVIEAENSTVVEVSDLHESDTTSR"
     gene            15756..16721
                     /locus_tag="PLB46_00340"
     CDS             15756..16721
                     /locus_tag="PLB46_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74985.1"
                     /translation="MEKEVIILFTKDRLETLKTTIESLCETPYKKFVIDDSNSSTNQT
                     GVSELCRRYKNFHYIGKLEFQSFVTLHNISLPKYSFLLRELGDINWNLGYARNFALLF
                     SKSASFDKVLFSDDDIIVTDNKLIQKLFHSLDTFKFAGANIAGLIDDSLLGYIATEMD
                     IENERTLSGGFMVYKPKIINHFFLNNYNEDWIWILLQMKNENLLQIGDVFQEIKDPFD
                     NYKSKVIFQEFGELVLDGVFNCYNGGSFDNLTNISFWDQIVKDRTEYLNMLMEKAHLN
                     SKGKSIEIINWVKLNSEIFHAEIFQNLFTQYAKDRLLFLKLYYSL"
     gene            complement(16709..16954)
                     /locus_tag="PLB46_00345"
     CDS             complement(16709..16954)
                     /locus_tag="PLB46_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRF74986.1"
                     /translation="MKKKLKPPKELVSEILEISIDQLNETSCYGETLNWDSISQAAII
                     NALELDYEIQIPDADIENYNTLDAIIKLYEKLKSQRE"
     gene            complement(16929..18392)
                     /locus_tag="PLB46_00350"
     CDS             complement(16929..18392)
                     /locus_tag="PLB46_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF74987.1"
                     /translation="MDFIERIKEKAKNSPERIAVIDGNKQISYADLLILVNTISNHLV
                     PKSKVLIDMKQCVESYALIIAVLNVGGTYCPLNPDAPSERKRQIIKEFAPDLIIVDSK
                     ETNFDIKSCTILTISNLLSTKSNKEIYLTHNGDDIIYIIYTSGSSGVSKGVQICRKAL
                     NKFLEWSIPTYGATENDIWGQFSLLSFDLSIVDIFTCLCSGATLFVIGDITAKTYRPD
                     RIIESAKITIWHSVPSTIEFMISNNRHKNCDFSSLKLMSFCGDTLKKYQVEFLFKKNN
                     NLRIFNTYGPTEGTLFCTWQELKINNYLNFANYSMSIGKAIPGWNLQLNSVEGFDDKE
                     ITIYGEFIGKGYLAPVANNKFRTIWLGNDEIEAFDTGDIVKEIDGNLYFICRKDNQVK
                     LKGFRVELDEVDFRIYEFLNLTSVTIVNNGTLYSFIESDQEINTSELREYLKTVLESY
                     KIPHSFYSIPEIPKNQNQKFDISALKSILNEKKAKTS"
     gene            complement(18394..19560)
                     /locus_tag="PLB46_00355"
     CDS             complement(18394..19560)
                     /locus_tag="PLB46_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74988.1"
                     /translation="MSNSFRTITIFVSHPFEPQNTTYNLELFRTNIKLLIKEAENEVR
                     KEYSEFEIEPVFEFSDFQNGLPQQIENKIRSSNFGIVDITENKPNIFFEYGLMRGLNI
                     PSLLIKGEDSFNTFGLPADIKDKIAISYKDFNDLQAKCLGNVVVLLKQLLNSDSLYNI
                     HLNKIWFRSDVGTIHVIAPAETEKSQYASPSSKNYIFLNNLGDIDSVLEIMNFLNRNY
                     RNTKLPIYSGDEFRNHMEDNLMVIGGPGETDGDGNNVSALLMNKMNVKVSYLFVDDDE
                     FMVYKNEKFSATYTGGKVTKDYGYFARFPNPFNPKSSVVLIHGIHTFGVLGAAKAFSD
                     HPSAQGNIRKVLKKLNLDDIKQASFECLFTVDVLQQSVVCPEINEEFILPLVKK"
     gene            complement(19557..20171)
                     /locus_tag="PLB46_00360"
     CDS             complement(19557..20171)
                     /locus_tag="PLB46_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74989.1"
                     /translation="MKSYKEIGSGIFVHENVRIGKNVTIGHCSCIGFGDPEGREIIIN
                     DGVTIGAFCIIHFGAIIKENVNIDHRCVIGEEVQIGNKTLVLSGKEISWRAKIGDNCI
                     IGGNVADRTIIEDDVTFFGEIAHTHKDAGKDWDTTIEPSPTIYKGSVIGVNSIIIGPR
                     KIGPCAYIGAGEIVRTDIGTSHALMKGEIKPLSFYRGMFKTRCL"
     gene            complement(20168..20989)
                     /locus_tag="PLB46_00365"
     CDS             complement(20168..20989)
                     /locus_tag="PLB46_00365"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF74990.1"
                     /translation="MKETTLKFKSYDGYPLTGTLIKAEKEKAALLFVHGITSSRDELG
                     FHSDYAKYLSENGISTLRFDYRYHGGKIDGSSKLENLTLSGILNDIDAAFLTLKLNVG
                     LNVNSFFVIGTSFGGGLTAYWSDFNNKTEVKKIILNAPVLNYEDDVLDRNNLMENGSM
                     SNKAQKQLAQKGYVNSSGIHFGRGLLNELKYINGIRALKNLGNKVVIFHGNDDDDVPI
                     MSSKKYKSEETRLEIIKKVGHGFGVENDEDLNYPETKAIHRDIYKKALEIIEGSL"
     gene            21491..22099
                     /locus_tag="PLB46_00370"
     CDS             21491..22099
                     /locus_tag="PLB46_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74991.1"
                     /translation="MIEDIMFTFDKNFLRLNNLIGLYRSLSTGSGRRPTNSLDVLRAT
                     TVLAHSTLEDYLRNLLMWRLPLQNRDKITHIPLTGTSANGRPTKFSLGDLVAHRGETV
                     DNVIMSSIRDYLGMQSFNDTSEIAAALSSISLPVTPEIQDIFPTLNEMIIRRHNIVHR
                     ADRDVNTGRGHHKIKSISIQKVEKWKKSIDQVVFEINKNFIS"
     gene            22108..22434
                     /locus_tag="PLB46_00375"
     CDS             22108..22434
                     /locus_tag="PLB46_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HRF74992.1"
                     /translation="MGKFIITRRTNGDYQFKLIASSGQTILVSEGYTTKKACENGIQS
                     VVKNSQDETNFIRKISTNGLYYFNLKAPNGQIVGTSEMYASESGRESGIFSVKLNAKA
                     GLVETK"
     gene            complement(22573..23535)
                     /locus_tag="PLB46_00380"
     CDS             complement(22573..23535)
                     /locus_tag="PLB46_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74993.1"
                     /translation="MRKCFVLRPFSDTTIMVNGKQAKIPKLEWEHIYNVWIKPSVETF
                     PDEKIECKCSELLPGNFLRGILSDIYSSDFVIADLTGQKPNVYYELGIRHALRMGTII
                     ITQDLNALPSDLRAYSCIEYNYPRYKSTNQDKINLFASNLHKNLRSILKNNFASDSPV
                     SDLLQLNHFFAQHFEMTEIKNFYAFILRLEALIKSYFDILKRIKPQRLECLTKNSFPT
                     EIINIARLNSLYSGFNNLPSTDIISRGFMDFETKIFSISDVFNSLYHYWDIYGKNINK
                     TNTDKYFSMIDKLISDEEKYIEMFIKLKEKFKGLYFKKFKIELQ"
     gene            24342..24563
                     /locus_tag="PLB46_00385"
     CDS             24342..24563
                     /locus_tag="PLB46_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74994.1"
                     /translation="MKNHKIAFELASTLDDNESIKIYEGFTERFTEDFLRRTLARVMA
                     IPDRKIKKTRGALFTYLVNQDGRSRNSRD"
     gene            24535..25017
                     /locus_tag="PLB46_00390"
     CDS             24535..25017
                     /locus_tag="PLB46_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74995.1"
                     /translation="MAEAAILGISPGTKYCGVVVLHHGELLVSKVTSFKGAWSEEKLD
                     KIASHVFTLVKKHAIAHIACKVHHASRTSVQVELILLKIKEMAKAIEIEVYTYTISEL
                     KGLFKMHFHSAHVLSEHIVRRFPELTDVFFQERKNKNKYHYKLFEALAAGLMCHSNLV
                     "
     gene            25172..25666
                     /locus_tag="PLB46_00395"
     CDS             25172..25666
                     /locus_tag="PLB46_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74996.1"
                     /translation="MKEIPASEIGQYLGLYPPLYQLVKNAHYEQGSLHAELIPFIYPF
                     TTEDLDYITATQIHLFLSQLTYVLIGKSITDPDFTLLSKIVTYDDYANKMHAGRLFFA
                     KLEQTMKRVIYKKNMPIYAEMKILNARRVGKTGFCEVEFDMNEGACFGKVLLSVQQIS
                     ATND"
     gene            25842..26489
                     /locus_tag="PLB46_00400"
     CDS             25842..26489
                     /locus_tag="PLB46_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF74997.1"
                     /translation="MIIKYINDTLDIIESLAIVFAAGTSIYAINTWKREGLWKKRSDL
                     AEEVLLNCYDVNNKLGEIRSPGSFPSEGKTRLENTNENRIEKEIRNWAYVPIERLNNR
                     REPFLNLEKLKLKCKIYFVPEVLVHIDQILNTQNEIINASRRFARLSIEQEKLISIQL
                     HGNENQERINYLQNQIAEAEAILSEDYFDEDKMKKTVKECISHLEKILLGEINAK"
     gene            complement(26520..27431)
                     /locus_tag="PLB46_00405"
     CDS             complement(26520..27431)
                     /locus_tag="PLB46_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HRF74998.1"
                     /translation="MRYAINQDGEKIEVIKSGDVAYCPGCKGKVYGRKGDFNTPHWYH
                     KTKECDTWYEPMTQWHIDWQNKFPLENREVVIFNNDNTIWHRADIKTNKDKVIEIQNS
                     SIDIARIYEREQFYNKNNLIWILNGASLASNSIVQSSFKPKGFEISLEIPTHIENVSN
                     YNMDDFQIALRENSVFKELQNHTFIKEFEVKRGWYYFWSFREFVDTRTYTEMLEVEVQ
                     RLFRELYGFGAYYKCKEKFSYSHYLNSTSYYYDIHLVKKNWRKFIDIMDAKVYLDNLN
                     GLPTDMLLDYRNNTFLPKNEFICRMLE"
BASE COUNT         9138 a         4766 c         4723 g         8919 t
ORIGIN      
        1 cctgagtgaa ctcggtttag cgatttccga aaatgagttt agtaacctca tttatggtcc
       61 ggatccacat ccggtaatca aaaattacta cgttgggcta tcacaaactg gtcagtacga
      121 cccgtctgta ttgccaggat ttgttgaaaa tatttcaaat ccggaaacac aacaaaacaa
      181 tccgcaagca cgccaggaat attaccagtt tgtttctcgt gaacaagtgg caaaacgcga
      241 ttacaaacaa actaaataca tgagtttgtt tacaaaatct gcttacgttc ctgaatggtt
      301 agctaaacgc agttatactg tttcaaatac acgtggcaca tttacaatga tttcgttgcc
      361 ttatactcaa attgccgact caactattac cactactgat gcggatttga aaaaatacta
      421 caacgaaaac aaaaataaat tcaaacaaac agaaggtcgt attgtagaat atgtaagttg
      481 ggaattttta ccaactgcaa aagacagtgc tgaaacatta aaagcattaa atgaaaattt
      541 agttaaaatg gccgcttcta aaagcgactc agcatatatg attgctcgtt ctgacgatcc
      601 tgataaattt ggtaattctt attatagcag aaacgattta tatgctcagg gtatcgactc
      661 ttcagtagtt agcaattttt attcgcaagc agttggtgca ttagttggtc ctttcgaaaa
      721 tggtggtatg tatcgcgtag ctaaaattaa aaatcgcagc gcaatgcccg attcagttaa
      781 agcgcgttta attgtggttg gcattacacc tgaacgcgat agtattaaag cgaaaggttt
      841 agcagatagt ttattagctg tattaagcgc aggtggcgat tttgctgcac ttgctgctca
      901 atattctgac gaccaacaat ctggtcaaca aggtggcgat attggttggg ctactccttc
      961 catgaattat attcctgaat tcaaaaaata tttctttgaa acaggaacaa ccggcaaaac
     1021 ttcaattatt aaaactgcaa atggttacca aataattgaa attaccgaaa tgtctgatag
     1081 aaaagaatta gtgaacgtag catttatgag taaaacaata agtgccggaa aagaaactat
     1141 cgattctatt gaaaaagccg ctactgcttt ctacgaaaaa tatcaaacac ctgaagattt
     1201 tgaacaaggt gtaattgata acagattatt taaacgtgta actcaaccgc ttgcaaaaaa
     1261 tatgtatgaa gttccgggaa ttgaaaatac ccgcgaaatg attacctggg catttgatgc
     1321 tgcaaaagat gaattcaagt tttttaatat gagtgaacga gtaattgttg cttatgttaa
     1381 agaagttcgt gtaaacggta ttgccgattt agaaaatgta agagaaattg ttgagcagga
     1441 agttattaaa gagaaaaaag cagagcagtt aggtaaacaa attaaagatg ccatgagcgc
     1501 aggttcaatg gatgctgttg ctgctaaatt aggcgttcgt gttgatactg ttcgcaatgc
     1561 aagtttcggt tcaccaaacg caccaatttt aggtcgcgaa ccaaaagtta tcggaactgt
     1621 attctctacc gaattaggca aaaccagcaa tgtagttgca ggtgttcgcg gtgtttatgt
     1681 tgtttctcct attgagttta caccggttcc tgaaacttca gattatacca tcaacaaaaa
     1741 tcagttaacc tatactatgc aaaataaata tcagggtcaa accttgttaa acgagctgaa
     1801 agaaaaagcg aaggtaacag acaaccgata tttatacggg gagtaaactt ttttaaggat
     1861 actttcgttt agagcttaag gcactgaaca tttttcccgc agatttcgtc agaagcagtg
     1921 aaatgccggc gccatctgcg ggaaattttt atcccggaat tgagtatatt tgactatcga
     1981 caaaacaaaa caagtggaac tcgtaaataa agtagcacaa agcggcatca tttcattcga
     2041 tttggaaacc tattttccgg acgaaaaacg tattgtcagc ttcgacctga aagattattt
     2101 atttaaaggt ttaattcttc gtgaaaaaga ttttcgtgaa gcattggctg caataaattg
     2161 ggaagattac acagggaaat atatggccat tttttgctcg gcagatgcta ttattccaaa
     2221 ttgggcgtgg atgttaatag ttgcacaagc tgaacctttt gcagcaaaaa ttgtgatggg
     2281 aactcccgat gatttgatta aacaaatgta tagagcaaca ctcgcgacaa ttgatattga
     2341 aacttttcgt gatcaaaaaa taattattaa aggatgtagc gacaaaccgg ttccggcgga
     2401 tgcttatctt gaaataacaa aaatattacg tcccgtagct aagagtatta tgtacggaga
     2461 accatgttca actgttcctg tttttaagaa aaaataattg ctttgaaaaa aattcactat
     2521 ctccttatcg gcgcatttgt tggtgttgca atttttattg gttacgctgt atcaggtaac
     2581 gacaaaacaa ccaacaacga aaatattgct gtagcaaccg acacgctgcg ggctttggct
     2641 gatgatgtaa ccaccataag atattttgcc gctaaaatgc ccgaaagcac tacgtttgca
     2701 ggcgaacctg ttccactaaa tgacattgaa gtgcgtgaaa gattggaccg cgaattgacc
     2761 gtaaatggct actggcattc aaatatgata cagaatttga aattagccag cagatggttt
     2821 ccggttattg aaccgatatt aaaagaaaat gggataccag acgattttaa atatttagct
     2881 attgccgaaa gtggtttgcg caatgtaatt tctccatctg atgcacatgg ttactggcaa
     2941 tttttaaaag gcaccggttt acaatacgga ttaactatta atgcagaagt tgatgaacgt
     3001 tatgatatgg aaaaatcaac tttagccgca acaaaatatc tcaaagatgc ttatgctaaa
     3061 tttgggaatt ggacattagc cggtgcaagt tataatgctg gaatgggagc aatagaagga
     3121 gcagtgaatt atcaaaagga agatgcgtat tacgatttat atctgaaaga tgaaacatca
     3181 aggtatattt tccgtgcact ggcttataaa atcatatttg aaaacacaca gaaatatggt
     3241 ttctttttga caaaagacga tttgtataat cctttgcaat accaaactgt aaaagtggat
     3301 agtaccatta ccgaccttgc cgattttgct caaaagcaca acactaattt taaaatgctg
     3361 aagtattaca acccttggtt gagaagcact ttactaacag ttaaaaaagg cgaaacctac
     3421 caaattcgtt taccactaac tgcaaccgtt aaaaccggag gcactcaata agtacgtttt
     3481 ccaatcacaa tcaaaatctt tatttttacc gttgtaactc atgacggtaa aaaaactcag
     3541 cgtaattgta cctgcctata acgaagaggc aacaatagcc accatcttaa ccaccttaag
     3601 taatgtgcag ttaatacatg atattgagat ggaaattatt gtggtgaacg actgttcacg
     3661 cgataatacg gcggcaaacc tggctgcatt tatggcagca catccacaag taaatatcag
     3721 ggcttttcac cacgaaaaaa acaaaggcaa aggcgctgcg ttgcacaccg gtattgcaaa
     3781 cgcaacaggt gattttacag ttgttcaaga tgcagattta gagtatgacc catgggaatt
     3841 caacatttta cttaaaccta ttgttgagcg caacgcagat gttgtgtatg gcagtcgttt
     3901 tatgggtggt aaaccacatc gcattttatt tttctggcat tcgataggga acaaattttt
     3961 aacgcgctta tctaacatgt tcagcaacct gaatttaact gatatggaaa catgttataa
     4021 gctggttcgc acttctattt tacaatctat tacgcttaaa gaaaatcgtt ttggctttga
     4081 acctgagctt acacaaaaac tggcaaaggt taaaaacatt gctatttacg aagtcggaat
     4141 ttcctattat ggccgtacct atgctgaagg aaaaaaaata aattggaaag atggcgttag
     4201 agctatttat tgtataattc gttatggctt atttaaataa atttgaattt acccaaactc
     4261 gatattgttt taccatgcta taatccggcg aataactggg tgaatgccat tattgaatcg
     4321 tatcaggcac tttcaacatt gttacctcaa acagccatta aaataatttt agtaaatgat
     4381 ggttctacca aacaaatcgc tgaaagtgat ttaacacaat tacaacaaca aatagttgct
     4441 tttcaatata tccattatcc ggtaaacaga ggaaaaggtt atgctttacg taaaggtgtt
     4501 gcagcaacta atgctcccat ttgtatatac actgatgtag attttccata tacaacaaat
     4561 agtataatgg atttattgca acaactcaca agtgcacaat ttgatattgc cgccggtgta
     4621 aaaaataaaa attattataa acatgttcca ccattcaggc gatttatttc taagttgtta
     4681 cgcgcatgca ccggttttgt gttgcgttta aaagtagctg atacacaatg tggtttaaaa
     4741 ggatttaatg aaaaaggcaa ggaattattt ctttccacca ccattgatcg ttatttattt
     4801 gatttggaat ttattttttt agcttctaaa aataaagaag taactattac acctgtgccc
     4861 attgagttaa atgaaaatgt acactttagc agcatgcgtt taggtatttt atttaccgaa
     4921 gcaaaaaatt tcatcaccat attttttaaa aattttaaat aatgcatatt ttacgcaaac
     4981 acccattggt agtactcatt gtgatgagta taataataat ttgtggggtt tggagtaaaa
     5041 ttattttcca cccaaacact tacttattgt cctctggtgg tgatgcttct aaaaacaatt
     5101 ttaccccagc ctggtttgtg aaatacgatg aaggcaccag gtttaccggc atgcattatc
     5161 cttatggcga acatgtggta tataccgata atcagcctgc tatttcatgg gctttaaatt
     5221 taatcgataa caatatttat cctattcata attataccgt aggtattctg aatattttat
     5281 tatttgtctc cattttattt ggtattattt ttctgtttcg cattttaacg tattacagat
     5341 taccgggctg gtacgccgct ataatggcgc tggctattgg attgtttacg ccgcaattgg
     5401 atcgattctg gggacattat gcattgggat atgccgtttt tataccctgg ttatggtggc
     5461 gattaattaa agtaaaagaa aataattata aactaattac catttttttc accatactgc
     5521 tcattacctt ttttggattt atacatatgt attatgtatt aattggggca ttaatggtgg
     5581 gatttcatgc atgcgttgtt tttattaaaa ataaaaggca atatatgcag gcgattaaat
     5641 tactggcaat tgccggaatt ccgttgctgg tggtaatgtt atttatgttt tttaccgacc
     5701 cggttaacga cagacctgaa actccttatg gatttttcag atataaagcg aatttccaaa
     5761 cggtatttct tcctaaacat ggcaccttaa ctaatcaggc aaaaaaatta ttccactttg
     5821 gtcacgccga tgcagaagga tatgcatttg ttggctatac aggatttatt tttttattta
     5881 ttatcctgtt tatttatttc aaaaggttat ggtttaaacg ttcagtacga aatgctttat
     5941 tgcctatgcc cggagattta ggagcgtttt ttattaccgg tatttgtatg ttactttttt
     6001 caatgacgat tccatttaat ttgggtttgg aatttttgtt agatatactt acccctttaa
     6061 aacagtttcg ttctccggga agattcgcct gggtattttt ttatatttat ttagtgggta
     6121 ttgcagtact tatttatgta atacataaaa agatacaaaa acggtatccg aaattagcca
     6181 tcgctttttt aggtgcattt actattttat tattggttga aggattgtcg tggtttagtt
     6241 taactgcaaa aggaattcaa actaacaatg aatcaaatcc ttatgatggg aaaaatactt
     6301 tttttgccga tatgttcaac ggcacagcat attcccctga tgattttgat ggcattttat
     6361 ttttgccttc catttttcag ggatcagaaa aattatatat agacagaacc ggttggggta
     6421 attttccaaa agcaatggaa ttgtcgtatc aaaccggttt gccattagta agttatatga
     6481 tgagtcgcac atcacaatcg gaaacattta atgatgtatt aatagctgca catccttaca
     6541 taaaagtaga gtcaactgaa ggggtatttt ccttaaaaaa actcgtcatc atgaccaccg
     6601 accggcccaa aacaattggc gaaaattata ttttaaatca cgcaacaaaa ttcagcagca
     6661 aagcgggctt cgatttatat gcatttgaca tggcctccat aaaagaaaaa ggtcctgcat
     6721 tagcaataaa cgactatttg caggtaaaag acagtctgca agtatttacc tcgccaacag
     6781 gaacagttta tcaggctgaa aaacctttgg aagtgttata ttatgacagc ttcgacgggc
     6841 aggcaagcga gcgggtgttt ttaggaaaag gagccttaaa caataagcgt gaggctgttc
     6901 ctgtggcaga tttcgctgtt tcgatatcgg acaccatttg gctggaagca tcttgctggg
     6961 ccaccaacga tatcagcgag gttgcctatc cctacttagc catctattat tatgacgaag
     7021 cgggtaacat ggtaaaagaa ttaggcattt cacccactca aacctgcgat gtgatgggca
     7081 attgggtaag agcctccgat aattttgaaa tttacccttc tataaaacgt atcaaagtgg
     7141 taagcaccga caacagtcag gcctggttcg acgaactcct cattcgccat tccgcctaca
     7201 acgcctacta ccacgtggtt tcagacagta attttgtagt aaataacttt aaaattggaa
     7261 aataattcca aaatcagtac ctttgcaatc ccttagaaaa tcaattcgac gtaggagaat
     7321 tagattttct tggtccgccg aggcggatag agtttaaatg gtgcggtagc tcagttggta
     7381 gagcaaagga ctgaaaatcc ttgtgtcggc ggttcgatcc cgccccacac cacccacaaa
     7441 caaaggcttt gaagagaaat tcttcgaagc ctttttattt tggtacaaca aaagtacaac
     7501 aatttgccct atacaaaatt tattatattt gaggtatgga agtcatttgc cttgaagatg
     7561 aagcgtttta tgcacttgta gaaagtgttg tacggagaat aaaagaacag caagggataa
     7621 aagaggataa atggatttcg actgaggagg ctatgaataa gctccggatt aaaagtaaaa
     7681 cgactctcca gaaattaaga gatgaagggc agattcgatt tacacagccg gaaaagaaga
     7741 taattttgta tgataccgat tcaataaacg agtatttgga aaaacattca aaagacattt
     7801 actaatggaa tttaatgaga aacattttat tttaggtttt aatagtggtt acattttagc
     7861 aaatcatgaa ccaatagtgc tatcaagcgt cataaaagaa ttgcaatctg ataactcata
     7921 catcaaaggt ttgttgcaag ggcaaataga attcgaaaat gagcaatcca aattcagatt
     7981 agatgaactg cgtcaatcta gaggaaaatc gatagatacc aaagaaaaag atttatagta
     8041 ggcaacgaag ttgtttatac aagtatttgg aaaacacctt atttaaaccg tttcacatgt
     8101 gccttataaa ttaccttttt gatttaaagc caaattagca gatttttagg atgtcaacat
     8161 gacaaattga cattattttt gatatttaat ataataaaaa aaatatttag tttttctttg
     8221 ctatttcaaa taaataaaat atctttgccc tccacaattt ttggaacagt tcttgtctga
     8281 ctgttatcca cgaatatttg ttaattacga gtaaggcttc acttattgca agtcttatct
     8341 ttgaagataa taggacccac cacgcttgct gtaactaagg ttacgtgcag cgcatttcgg
     8401 tgggttattt tttttatgga gaagaagccc tttactaaac ctcacctcac atttcacgag
     8461 cagttggaaa tgttaaaatc tcgcggatta atgattcaga atgaaggtga aaccctgtcg
     8521 tttttaagag gagtaaatta tttcagaatc atcccttatt gccggttatt tgagtatagg
     8581 attgaagaaa atccagatga gcattttttt aagccagaaa cttcgtttga aaaaattcaa
     8641 aggcactatc tatttgatag agaattaaaa ttgttagttt ttgacctaat tgaagaattt
     8701 gagattggtt ttagaacgca attcagtcat gaatattcta aaatcgaaaa cccagatgca
     8761 tactttgatg atagtggaaa ttttattcga aaatggtggt atgaggattt aagtttgtat
     8821 agaaagtata aactttatca aaaagatttc aaaacaataa attttgaagt agatagagca
     8881 aaaaatgatc cctttattat gcactattgg aataaatatg aaattccggc cacattacca
     8941 tcttggatga tgtttgaagt aatttctatg ggtacgttat caagattgtt ctccaattta
     9001 aaaataactg agccgaagaa gaatcttaca aacttttata aattacccag cccacatatt
     9061 ttggaaaact ggttgtataa tatagttttt gtaagaaata tttgtgcgca tcatggtcgc
     9121 ttatggaata aaagcttaaa aaccgtagtt tttccactta aatctgaaag atgcaattgg
     9181 ttattaaaca cggaaatatc ggccacaaaa ctctactcgc ttttgagtgc acttttatac
     9241 ttacaaagta gtatctctaa aacttcagtt tttattacac gttttaaaga tttattacaa
     9301 aggtttcctg agattgacac agaagcaatg ggatttcctt taaattggga tcaagaacct
     9361 ctttggaatc aaattactta agataaaagg agggtattat aaataattct aactatttga
     9421 agcaaaaatt acccaatagc aagtagcccc ttgagctcaa ttccttctca acttttattt
     9481 ctttttcccc gtaatctacc taaactgttc gcgctgaaaa aaaattctct gaagtgccat
     9541 caatttgaat ttttaagtaa atctctgttt tgcctagccg aagcaggtct aatggttcaa
     9601 tttcattact aatctctttg gccattaaat ttgcatcgtt tgctcctagt ctaaaacaaa
     9661 taattgatcc aacattacca aaaatcgcag accgtatttc ttcatttatt tgttcaatgt
     9721 attgatgcgc caagaaaagg gatagtccgt atttccttga ttcagcgagg atatctgcaa
     9781 atgaaagtga tagaaatgaa tgtgcctcgt caacataaag ataaaacgga gtacgtttgt
     9841 ccattggttg ttttgctctg taaagtgcag aaagttgaat ggaggtaacc aaaatactcc
     9901 cgataatagt agaagcatct tccccaagtt caccttttga aaggttggca atgagtatct
     9961 tttgttcatc cataacctgt gacattcgaa aacctgacac ctgcttacca agcatgtttc
    10021 tgagcggttt acttgatata aaaacaccca ttttgttgag aatcggagaa atgacttcat
    10081 tccggaatga tggtgtatag gtgtcaaact cttttctcca aaagtcaaaa atgtaatcat
    10141 cttttacgta ttcaagcacc ttttctttga aatgtttgtc ggtaaggagt ggttgaatat
    10201 caagcaatgt tgcatagggg tattgaagta gtgtaaggag gctaaaacgg agtatgtatt
    10261 ccattctggg gccccatgat tccgaccaga ttttgcgaaa agttgaaagt aatcccgaag
    10321 ctaccaagct atgataattc ggatgcacag catgaagtgg attaaaagca atcacatttt
    10381 catcaacggg attaatatat accacatcct tcttcctctc ctccggtatt cgttcaagca
    10441 ggtattttgc cacatctcca tgagggtcaa ttactccaca acccttgcct ttggtaatgt
    10501 cagaaagagc cattttttgt agtagtgtag actttcccat tccagtcttt ccgatacagt
    10561 aaatatgccc aagtctgtcc ttgtctttta taccaaaagg aatgttttgg ttcctccagg
    10621 tggtatatcc tattggtgta atgtcataat catccgtact catactggat attgtagaaa
    10681 attcagttat tcagcataag tggggtaaaa ttgctatttc cggcaaattt gaccccctta
    10741 tctcaacatc ttctgtaatt cacaataagc gggtactcac aaaagtaaaa cggtacgtta
    10801 gtatatacat gaagaaatca aaagacggtg aaaatgacat attaagcggt tttaacccaa
    10861 actcaccttt ggtcaccgat gacattgctc ttgattactt ggccgaacta ttagtagata
    10921 tttatttaga cgaattggag catgcctccc tatccacaaa acacaactaa accacaagct
    10981 gttatatatt gccgggtttc cacaaaacaa caagtagatg aaggaaactc actttcaacg
    11041 caggaacgat tatgcagaga atatgcactg aagcaaggat atgaagttgc tgctgtatat
    11101 atcgaaaaag gagaaagtgc aaagacaaaa gacagaactg agcttcagaa aatgatgtct
    11161 ttttgtgcta ttaaaaagaa caatatttcc tatgtagtgg tgtataaact ggatcgtttt
    11221 tcaagaaata cagatgacta tagctatctc agaataatac tcaaaaggta tggaatattt
    11281 ataaaatcga cgaccgaata ttttgagaat actccggctg gaagatttat ggagaatatt
    11341 atttccaatg tgtcccaatt tgataatgat gtccgaaccg agcgagccgt taatggtagt
    11401 agagatgcaa tggttgaagg aagatatgtc tggtctgcac catatggcta cagcaatatt
    11461 aaagttggtg gtagaggtaa cattgctcca aatgataatg cgccaatagt aagacgagcc
    11521 tttgaagagg ttgctaaaaa tcaataccca atagaagaga ttagaaaatc tatgtttcat
    11581 tccggcttaa caacttatat gaataaaccg ctatcacgag cccaatttta tagaatgctt
    11641 aaaaatgttg cctattccgg ttggattgtg aaatttggtg aaaggcacaa agggatatat
    11701 gaaccaatcg tttcagatga gctatttgaa caagtgcaaa gagtgctcaa ttaccgaacc
    11761 cgaaaaaact tcacctataa ctgcaagcat cctgattttc cattacgccg gtttgttact
    11821 cacccaaatg gaatgaaatt gacaggtagc tggaaccaag gaaagtcaca gaaatatcca
    11881 tattaccggt atatgtgtag aatgatgaat ttcccaaaag aatcgcttga gagccagttt
    11941 atggccttta tggacacttt taaactggaa ggtggccagt atgatgcgtt aaagaaaaaa
    12001 ctgaaaggtg cacttgaaaa aggggtgaaa tatcgactaa aagatagaga taaagtactg
    12061 caagaggtaa aggaattaaa gcttaaacag tctcttttaa ttgagaagaa tatacaaggt
    12121 gtaatttcca acgagctact ccgcgatcaa cttgttttaa ttgataaaca attactggaa
    12181 ttaaattcca aattatacat tgaaccgacc tatgaagtga atctagaaga gcttattacc
    12241 aaagcaaaaa cactcctcac aaatcctagt gaagtttgga aaactgcaaa taatgacaat
    12301 aaattacgtt tgcaggtgtt tgaatttcca catggggtaa cctttgatgg agaaattttt
    12361 caaacaccgg aaatatgtag actgttcaag gcaaaagacc tttttttgcc tagttcgtct
    12421 cataatgcgg gcgctaagaa aaaagttatc aaatttcacc cagaagtagc aaatttaccc
    12481 tacataactg gggataaacc aagtccctgc gctacctata atcctaccac aaagaaatac
    12541 tgggaagaaa ttgtgaagga tttgatagta gtaaataata ttttgtcaga ggagttaata
    12601 gatgacacat aaaatttgct atttctagca aatttaccct ccttatacgt tcacaattat
    12661 ttcactaaaa taaaaccata tgaaaatacc aaataacata aaagaatatc gtttaagaaa
    12721 aggattaact ctgatacaag ccgcgaagct aattaaactc gattgtgcag accgtctctc
    12781 aaaatgggag cacgggcata agtatccaag tgttccaaac ttgtttcgac tttgtgccgt
    12841 gtatgaagct ctgcctgagg aactatatcc gatacaatcg atggaaccct ttttacttca
    12901 tcaagatata cgtcaacaag aatgtcgaca agctcatcaa gtaaccaatc ctcctcaggt
    12961 gtaatcttgc gagggcggcc agcatttctc ttaccagtct cccaaatctc aaggggtgtt
    13021 ttctttctac gtgttttcat aattaaattt atcttgatgt tgtatcactt tcatgcaaat
    13081 cacttacttc cacaaccgtc gaattttcag cttcaataac aggaggatct agtttttgca
    13141 cattcttaat tttttctaat acattaataa tagcatttgg tacttgttca attgtaataa
    13201 tgtatttttg aattatttgg gcacaaatac gtttcgaagg caagtacaca gtttgccctg
    13261 acttattagc cacaagagca gctaataatt tgtgaaattt tcgattctca attttagggt
    13321 caagctgccc ccttaatcca ttcaattcat catcggttac tcctataccg cacttttcct
    13381 gaactaatcg tatccatacc tcattaggaa ataaatcttc tatatcacat gttccaagca
    13441 agtaaacatt cgcgccagtc tcacctgtgt cagaatcaaa cacataatgc actgtgcttt
    13501 ctaccttttt aaaatcaccc aatatatttt ctagaatttg cttgttatgt ttatagttac
    13561 cagatccatt aagagggatc aattttatag cattttcctc taaagattta cccgagtgta
    13621 atttgtaaaa atgaggtaga agtacaatat ccgtttcacc ttcaacagct aaaaatagat
    13681 cataaaataa aatgtcgcta tttcgaactc caagaacatc atgcacatcg tctattgttg
    13741 tgcatttgag aatctcagaa tacttttctt tcaattttat tttatatatt tcattcaagc
    13801 gcaacctatc aataaaaatg gtagagtggg ttcctaaaat aatttgatag tttccttgag
    13861 ataaccccgc taaaatggca aacaaatctc gctgtgcagt cggataaagg tgtgattcag
    13921 gctcatcaaa gcaccatata aattgcttat gagatgtttc tgtttcttca gaatttcgtg
    13981 tatttgccca ttttagaaaa gcaaataata tttgtctttt cactccctca ccttgagaat
    14041 caagacgtat atcgccatct gaggtggttt tattaataat aatatcagaa actttttccg
    14101 caacagtaaa tgttactttc gcctttatgg cgctgatatt atttaaatct cgggttaatt
    14161 cctttgttaa agactcgaat tccttgttta cctcttcggt ggcatttttg ctaacgtcgg
    14221 cagcttgatc agctaaagct tttttagaat cttcaatgtg ttttttcatc acgtcattag
    14281 ccaaattttc aatatcagct agggaggtgt tccaatcaat atatctatac acaggaaatt
    14341 tcggtaagtc agctttaata aaactcggat attcactcca aactggcttc aatgttgttt
    14401 tgctataaat tccccggatt atttcaagat tcccaaagcg acctttgcta ttatcatttc
    14461 ttatgtcctc atcagtaaca cctaattctt tccgagcttg agtgagttct gttgcttttt
    14521 tattccataa cccaagaggt ttttcagcat catttgtata gagaaaatac ttaccggttg
    14581 agtcacctgg ttcataagct tttaataggt agattttctt gttggcaaaa ctctccagag
    14641 accatcgcaa ctgttctgat aaggactctt tgaattcgtc agtatattct tcatcttcaa
    14701 taatgaattc acccaatatt gctgatccat taatttgatc cgaaaatgga agaatgttca
    14761 gctttttaaa tatttccgca cattgttctg aaccaagcaa agtatttgat atgtcagatg
    14821 ttgctcttgt atctccggag atattaaaag gagttttagg atcaagaagg agtccgattg
    14881 attttataat agctgattta ccagaatcat taatgccaat tattgtagtt ggatcgtcct
    14941 taaaaaagtc tatagaaata ccctgacagc ttttataatt aaaaatggaa agtgtgttga
    15001 gatacataaa gcgtatgttt actcaaatct ataaataatc ttcgattttg ttgccctcag
    15061 cgatattttt atcaatgctt gtcaaggttg gcttctacag aatgcttatt tcttaacgtt
    15121 tttattgcag aatgtgttat ctcacattgc gggcgctaag aaaaaagtta tcaaaaacca
    15181 cccagaagta gcaaatttac cctacataac tggggataac ttaaacaaga atagcactga
    15241 aattgactgg gtacaaataa aaaacgatct gattaaacta tcggagatat taagaccgga
    15301 acaagtaata atcgacacct cctaaatttg ctacttatat caaatttacc cctcttgtat
    15361 attcctaatt atttcactaa aatgtaaccc tatgataaca ccaaacaact tcaaaaagta
    15421 tcgtttagaa aaaggactga ctttaaagca attacctgag tatttagggt tagattgtga
    15481 agaccgactc tcacgatggg aaaacggtca tacattggct ctcaaaaaat agaaaaatga
    15541 aaaaaattaa gctcattggt tcaatttaat taatcaaata aacattaaat tagcattgtt
    15601 ttaacgaagg ataatgaaag tattttatta gcatttcacc tcaataaaat cagcttttac
    15661 acccttttat actatattca tggaaaaacg aatttaattg aaactttaaa gcctaattat
    15721 caaacttttt aagttaaacc taaaatttac aaccaatgga gaaagaagta ataattttat
    15781 tcacaaaaga caggctggaa acattaaaaa ccacaattga atctctgtgt gagaccccat
    15841 ataagaaatt tgtaatcgat gattcaaatt cttctaccaa tcaaactggt gtttcagaat
    15901 tatgcagacg atataaaaat ttccattata tcggaaagct ggaatttcag agttttgtta
    15961 cgctgcacaa catatcttta cccaaatatt catttttgtt aagagagttg ggagatatta
    16021 attggaattt aggttacgca agaaacttcg ccttgttatt ttcaaaatcc gctagttttg
    16081 ataaggtact tttcagtgat gatgatatca ttgttactga taataaatta attcagaaac
    16141 tatttcactc acttgacact tttaagttcg caggagcaaa tatagctggc ctaattgatg
    16201 actcattact tggatacatt gcaacggaaa tggacatcga aaacgaaaga acgttgtcag
    16261 gagggtttat ggtttataaa ccgaagataa ttaaccactt ttttttaaac aattataatg
    16321 aagactggat ttggatatta ctccaaatga aaaatgaaaa ccttttacaa atcggtgatg
    16381 ttttccaaga aattaaagac ccttttgata attataaaag caaagtaatt tttcaagaat
    16441 ttggcgaatt agtattagac ggggtattca actgttataa cggaggatct tttgataact
    16501 taacgaatat ttcattttgg gatcaaattg ttaaagaccg aactgagtat ttaaatatgc
    16561 taatggaaaa ggcacatctt aatagcaaag gtaaaagtat agaaattata aattgggtta
    16621 aacttaattc cgaaatattc catgctgaaa tattccaaaa tctatttact caatatgcaa
    16681 aagatagatt attatttctc aaattatatt actccctttg actttttaat ttctcataca
    16741 acttaattat ggcatctaag gtgttgtaat tttcaatatc tgcatccgga atttgtattt
    16801 catagtcgag ttctaaagca tttataattg cggcttgact tatactatcc caattaagtg
    16861 tctcaccgta acatgatgtt tcatttagct gatcaatact aatttcaagg atttcagaaa
    16921 ctagttcttt aggaggtttt agcttttttt tcatttaata tactttttaa agcactaata
    16981 tcaaattttt gattctgatt cttcggaatt tctggaattg agtaaaaact gtggggtatc
    17041 ttataacttt ccaacacagt tttcaggtac tctcttagtt cagaagtgtt aatttcttgg
    17101 tctgattcaa taaacgaata aagggtgcca ttattcacga tagttaccga agtgaggttt
    17161 agaaattcat aaattctgaa atctacttca tctaattcta ccctaaaacc ttttaatttt
    17221 acttgattgt ctttacggca aataaaatat aaatttccgt caatttcttt cactatgtca
    17281 ccagtgtcaa aggcctcaat ctcatcattg cccaaccaga tcgttctaaa tttattatta
    17341 gcaacaggcg ctaagtagcc ttttccaatg aattctccat agattgtaat ttctttgtca
    17401 tcaaaacctt ctacagaatt aagttgaaga ttccacccag gtattgcctt accaatgctc
    17461 attgaataat ttgcaaagtt taaatagtta ttaattttta attcctgcca agtacaaaat
    17521 agcgtccctt cagttggccc atatgtattg aatatcctca aattgttatt tttcttaaat
    17581 aaaaattcaa cttgatactt ttttaaggtg tccccacaaa aactcattaa tttaagggaa
    17641 gaaaaatcac aatttttatg tctattgtta ctaatcataa attcaattgt acttggcaca
    17701 gaatgccata ttgttatttt tgctgattca ataatccgat caggacgata tgttttagca
    17761 gttatatcac ctattacaaa taaagtagcg ccggaacata aacaagtaaa tatatcgaca
    17821 atacttaaat caaaacttaa taaagaaaat tgcccccaaa tatcattttc tgtagcacca
    17881 tacgttggga tagaccactc gagaaactta tttaatgctt tcctacaaat ttgaacacct
    17941 ttagaaacgc ctgaagaccc tgatgtataa ataatgtaaa ttatatcatc accattatga
    18001 gttaaataaa tctccttatt gctttttgtt gaaagtaaat ttgaaattgt taagattgtg
    18061 catgatttta tatcaaaatt agtttctttg gaatctacaa taattaaatc aggtgcaaac
    18121 tcctttataa tttgcctctt tctttctgaa ggtgcatcag ggtttagcgg acaataggtt
    18181 ccaccaacat taaggaccgc aataatcaat gcataggatt ctacacattg tttcatatca
    18241 attaagacct tagattttgg aacaaggtga tttgaaatgg tattgactaa gataagtaag
    18301 tcggcataac taatttgttt attcccatca attaccgcga ttctttctgg actatttttt
    18361 gctttttcct ttattcgttc tataaaatcc atgctacttt tttactaggg gtaaaataaa
    18421 ttcttcatta atttctggac atacaacact ctgttgtagg acatctactg taaacaaaca
    18481 ttcaaatgaa gcttgtttaa tatcatcaag atttaacttc ttcaatactt ttcggatatt
    18541 gccttgtgcc gaggggtgat cagaaaacgc ttttgccgcg ccaagaactc caaatgtatg
    18601 gatgccatgt attaacacta cagatgattt gggattaaat ggattgggaa acctagcaaa
    18661 gtatccatag tctttggtaa cctttcctcc tgtgtacgtt gctgaaaatt tctcgttctt
    18721 ataaaccata aactcgtcat cgtcaacaaa taaataacta accttaacat tcattttatt
    18781 catcaataag gctgatacgt tgtttccatc accatcagtc tcaccaggtc caccgataac
    18841 cattaagtta tcttccatat gattcctaaa ttcatcgccg gaatatattg gcaattttgt
    18901 atttcggtaa tttctgttta aaaagttcat aatctctaaa acagaatcaa tatcaccaag
    18961 gttatttaag aaaatataat tctttgatga gggagaagca tattgacttt tttcagtctc
    19021 tgcaggcgcg attacatgga ttgtcccaac atcgctccgg aaccaaattt tgtttaaatg
    19081 aatgttatag agtgaatcgc tatttaatag ttgttttaaa agaacaacaa catttcctaa
    19141 acacttagct tgcaaatcat tgaagtcctt ataagaaatt gcaattttat cttttatgtc
    19201 agcaggtaat ccgaaagtgt taaatgagtc ctccccttta attaataaag acggaatgtt
    19261 taatcccctc ataagtccat attcaaaaaa tatgttcggc ttattctctg taatatcaac
    19321 tataccaaaa ttactacttc taattttgtt ttcaatttgt tgaggtagtc cattttggaa
    19381 atcactaaat tcaaaaacag gttcaatttc aaactctgaa tattcttttc tgacttcgtt
    19441 ttcagcttct tttattaata atttaatgtt agttctaaac aactccaaat tatatgtagt
    19501 gttctggggc tcaaatggat gagagacaaa aatggtaata gtgcgaaaac tgttgctcat
    19561 aggcatctgg ttttaaacat tcctctatag aaggataatg gctttatttc acctttcata
    19621 agcgcgtgag atgtgccaat atcggttcga actatttcgc cggcacctat atatgcacaa
    19681 ggtccgattt ttcttggacc tattataatt gaatttacac caatgacact tcctttgtat
    19741 attgttggtg atggttcaat ggttgtgtcc caatctttac ctgcatcttt atgagtatga
    19801 gcaatttcgc caaaaaaagt aacatcatct tcaataattg ttctatctgc aacatttcct
    19861 ccaatgatac aattgtcacc aatcttggca cgccaagaga tttcttttcc cgaaagaaca
    19921 agtgttttgt taccaatttg tacttcttcc ccaatcacac atctatgatc aatatttaca
    19981 ttttctttga taatggctcc aaaatgtata atacagaacg ctccaattgt caccccatcg
    20041 ttaatgatga tttcgcggcc ttcagggtct ccaaatccaa tgcaagagca gtgtccaata
    20101 gttacgtttt tgccaattct tacattttca tggacgaaaa ttcctgagcc aatttcttta
    20161 taagatttca taatgaaccc tctataattt ccaatgcttt tttatatatg tctcgatgaa
    20221 ttgctttagt ttctgggtaa tttaaatctt catcattctc aaccccgaaa ccatgaccaa
    20281 cttttttaat tatttctaat cgggtttctt cagatttata tttttttgaa gacataatag
    20341 gcacatcatc atcgtcgttt ccatggaaaa taactacctt atttccaaga ttttttaaag
    20401 cacgaattcc atttatgtat ttcaactcat ttaaaagacc tcttccaaaa tgtatccctg
    20461 atgagtttac atatcctttt tgcgctaact gcttctgagc tttatttgac attgacccgt
    20521 tttccataag gttattccta tcaagcacat catcttcata atttagcact ggcgcattca
    20581 aaattatttt tttgacttca gttttattat taaagtctga ccaatatgca gtgagtcctc
    20641 caccgaaact tgtgccaata acaaaaaatg aattcacatt tagccctacg tttaatttca
    20701 gagttaagaa tgcagcatca atatcattta aaattccact taaggttaga ttttcaagtt
    20761 ttgaactacc gtcaatttta cctccatggt aacggtaatc aaaacgtagt gttgaaatgc
    20821 cattttcaga aaggtatttg gcatagtcag aatgaaaacc caattcatcc cttgacgaag
    20881 tgatgccatg cacaaaaagt aaagcagctt tttctttctc tgcttttatg agtgtgccgg
    20941 tcagtggata tccatcgtaa cttttaaatt ttagagttgt ttctttcatt ttaatagcat
    21001 ttattagttt gtttattgta ccgatttaat aataacatgg tttatactgc tttagcacgt
    21061 cgatagtacc ctagttccat tttatcttga tgaaaaaaaa tattaaatga tttcaaattt
    21121 aatattatgt tcttgtaatt tacaataatt tacatttaat gcaacagatt cacggttttc
    21181 cgttaatcat attattaaaa tgacaaaaaa atcgccaata ttttcactta tcgataaaaa
    21241 cctccttttt agagcttgca ctctagtatt ttcagtagga ttaccttatt ttcttaattg
    21301 attcagaaat tgcaaacgtt attgtcaagt aactacgtat gaaaatgttg aaatgctaag
    21361 cgacttaatt attacagaat tgttacgaat ggggggatta ttcaaaaagg ctaattgtta
    21421 aaataaagta ttgtttgaat aatagtatat ttgacgatat ttaattcagt ttcaattgtt
    21481 accatcacaa atgatagaag atatcatgtt tacgtttgat aagaattttc taaggctaaa
    21541 taacctaata ggtctatata gaagtttgtc aaccggtagt ggtagaaggc cgacaaattc
    21601 cttggatgtg ttaagggcaa ccacggttct tgctcattca acattagaag attatctgcg
    21661 aaacctattg atgtggagat taccactcca aaacagagat aaaattactc atatcccttt
    21721 aactggaacg tcagcaaacg gcagacctac aaaattttct ttaggagatc ttgtagcaca
    21781 tagaggagag acagttgata acgttattat gtcttctatt cgtgattatt tgggtatgca
    21841 aagttttaat gacacttcag aaattgcagc cgcattatct agtatttcat tgccagtaac
    21901 tcctgaaata caagacattt tccctacttt aaatgaaatg ataataagaa gacataatat
    21961 tgttcaccgt gcagatagag acgtcaatac tggacgaggg caccataaaa taaagtctat
    22021 cagcatacaa aaagttgaga aatggaaaaa atctattgac caggtagtgt ttgaaattaa
    22081 taaaaatttt attagctaaa tttgcatatg ggtaaattta taattacaag acgaactaac
    22141 ggtgattatc aattcaaatt gatagctagt agtggtcaaa ctatcttggt cagcgaagga
    22201 tatacaacaa aaaaagcatg tgagaatggt attcaatcag tagttaaaaa ctcgcaagat
    22261 gaaacaaatt tcatacgaaa gatttctact aatggtttgt attattttaa cctgaaagca
    22321 ccgaatgggc aaatagtcgg aacaagcgaa atgtatgcaa gtgaatctgg aagagaaagc
    22381 ggtatttttt ctgtgaaact aaatgctaaa gctggtttag ttgagactaa atgaacctgc
    22441 aaagattata caggtaaaga ttgtgatctg attaatttat ttaatactct ccaatcaata
    22501 gattaaaatt cttcgctatt tatgtaaata tttgatgaaa acaatcagtt ggccattcaa
    22561 ttaacaaagt gattattgca attctatttt aaattttttg aaatagagtc ctttaaattt
    22621 ttctttcagc tttataaaca tttcaatata cttttcttca tcactaatta gtttatcaat
    22681 catactgaag tatttatctg tattagtttt atttatgttt tttccgtaaa tatcccaata
    22741 atgataaagt gaattaaaaa catcagaaat tgagaatatt ttagtttcaa aatccataaa
    22801 gcctctggat ataatatctg ttgatggtaa gttattaaac ccagagtaaa ggctgttaag
    22861 tctagcgata ttaattattt cagttggaaa agaattttta gttaggcatt ctaacctctg
    22921 aggtttaatc cttttgagaa tatcgaaata ggatttaatc aacgcttcta aacgcaatat
    22981 aaaagcataa aaatttttaa tttcagtcat ttcaaagtgt tgagcaaaaa aatgatttaa
    23041 ttgtaacaaa tcagatacag gactatcaga tgcaaaatta tttttaagta tacttcgtag
    23101 atttttatgt aagttgctag caaataaatt tatcttatcc tgattcgtgc ttttataacg
    23161 aggatagtta tattcaatac aagaataggc ccgcaaatct gatggtaagg cattaaggtc
    23221 ttgcgttatt ataatcgttc ccattcttag ggcatgtctt attcctaatt cataatagac
    23281 atttggtttc tggccagtca aatccgcgat tacaaaatca gagctataaa tatcactaag
    23341 aattccacgt aaaaaatttc ctggaagaag ttctgaacat ttacattcaa ttttttcatc
    23401 ggggaaggtt tctacagagg gtttaatcca cacattataa atatgctccc actctaattt
    23461 gggtattttt gcttgcttcc cattcaccat tattgtggta tcactaaatg gcctaagcac
    23521 gaaacattta cgcatttgaa gtaagtattg atattctttt gattaaaaaa ggattttcgg
    23581 tgaaataaca gtataaactg cattattttt gtcaattaag taatattaca aaaattaaat
    23641 tttaacaact ggagttttat tagtttctta ttcgtcataa taattaaatt ttcagctaat
    23701 ttaataattt tttatcgcat atcttccggt ctacatattt ttttaataaa aatttggcac
    23761 acgcagaaat atcgaaagtt ttccacagaa tttgctataa atagcaaaag tacccccctt
    23821 atgtcacttg actcaacttg atacgctgtc tgcatggaac ataaaggtcg aaatattcca
    23881 aacaatctta aaaaatttag gcggatggct ggatacaagc aggaagatgt tgcaatatac
    23941 ttaggactcg tgagcactaa cagaatctct cgttgggaga gtggcttggc tatgccgagt
    24001 gctcagaatc ttttcaaact aagtatttta tacaacaccc ttgttgatca gttatacagt
    24061 agtcacatta gtgaaatgaa acgcaacttg accaacaaag aaaaactgct tgtttccaga
    24121 catcatcaaa agaaattgaa agaatagtct gtattcttgt tctttttgga ggcgtaacag
    24181 tatatactgt tctagccttt ttattgtgca aaaaattgaa aaaatcacct gtggaaaaac
    24241 cataacgtcg tcgtaaacgg tcggtttatt tatcttaaat aaataaacat cgaacgagta
    24301 taagttttcc acagatttta cccatcgtag ttatttaaaa aatgaaaaat cacaaaatag
    24361 cattcgaact tgcatcaaca cttgatgata acgaatccat aaaaatatat gaaggtttta
    24421 ctgagcgttt tacagaagat tttcttcgca gaacattagc tcgtgttatg gctattccgg
    24481 ataggaaaat aaaaaagaca cgcggcgcat tattcaccta tctggttaac caagatggca
    24541 gaagccgcaa ttctcgggat tagtcctgga acaaagtatt gcggagttgt cgttttgcat
    24601 cacggtgagc tattggttag taaagtcact tcgttcaaag gagcatggtc agaggaaaag
    24661 cttgataaaa tagcttctca tgtctttacc ttggtaaaga aacacgctat agcgcatatt
    24721 gcttgcaaag tgcatcatgc ttctcggaca tcagtccaag ttgagttgat tcttttgaaa
    24781 atcaaagaaa tggcaaaagc aattgagatt gaggtatata cttacaccat aagtgagcta
    24841 aagggtctct tcaaaatgca ctttcacagt gcccatgtcc tgtcagagca tatagttcgc
    24901 aggttcccgg aattaactga tgtctttttc caagagcgaa agaacaaaaa caagtatcac
    24961 tacaagctct ttgaagcact tgctgcaggc cttatgtgtc attcaaattt agtttagttt
    25021 gaaatgtatc attgtaagca caaagacggt ccacaaggcc gttttttgcg ttttaacgca
    25081 tctatttgat tgattgagta atttacctac cacctattgc aaaatcgctg taattggata
    25141 tatttgacaa atgagcaaac ctaaaccaac tatgaaagaa ataccggctt ctgagatcgg
    25201 gcaatatttg gggttgtatc cgccactgta ccaactagtc aaaaatgccc actatgaaca
    25261 agggagcctt catgcagaac tcattccgtt tatataccca tttaccactg aagatttgga
    25321 ctatatcacc gctacacaaa tacacctgtt tctgtcacaa ctcacctatg tgctcatagg
    25381 taaaagtatc actgacccgg attttacact actttcaaaa atcgtgactt acgatgatta
    25441 tgcaaacaaa atgcatgctg gccgcctgtt ttttgccaaa ttggagcaaa caatgaagcg
    25501 ggtcatttac aagaagaata tgccgatata tgcagaaatg aagatactaa acgcgagaag
    25561 ggttggcaaa actgggtttt gcgaggtgga gtttgatatg aatgaaggag cttgctttgg
    25621 aaaagtatta ctctctgttc aacaaatttc ggcaactaac gactgataaa gtttttgatt
    25681 tggagcttat attaattgtt ttcaaaacaa attgaaaaaa ttatctgtca actattgtgc
    25741 cagtttggtt atgactcgtt ttgcttttta tatatcctta taattagatt taaggacgtg
    25801 caattttgtg gtaaatagaa aaaataatag gtttgtaaat aatgattata aaatacatca
    25861 atgatacgtt agatattatt gaaagtttag cgatagtatt tgccgcaggt accagcatct
    25921 atgctataaa tacctggaaa cgtgaaggcc tttggaagaa acgaagtgat ttggcagagg
    25981 aagttttact gaattgctat gacgtcaaca acaaattggg agaaatcaga agccctggtt
    26041 cgtttccctc agagggaaaa accagattag agaacacaaa tgaaaaccga atcgagaagg
    26101 aaattagaaa ttgggcctat gttccaatcg aaagattaaa caataggcgc gaaccttttc
    26161 ttaacctgga aaagttaaaa ttgaaatgta aaatatattt tgttccggaa gttttagttc
    26221 acatcgatca gatattaaat acacaaaatg aaattattaa tgcctcaagg cgttttgcaa
    26281 gattgagtat tgaacaagaa aaattaattt ctatccagct gcatggtaat gaaaatcaag
    26341 aaaggataaa ttatttgcaa aatcagattg ccgaagctga agccattctt tcagaggact
    26401 atttcgatga ggataagatg aagaaaacgg ttaaagagtg catatcgcat ttagaaaaaa
    26461 ttcttctagg tgaaataaat gctaaataga ctattgaatt ttgtttcaaa cagcaattat
    26521 tattctaaca tacggcatat aaattcattt ttaggaagga aggtattatt cctataatcc
    26581 aggagcatat ctgttggtaa gccgtttagg ttatcaaggt aaactttagc gtccattata
    26641 tcaataaatt tccgccaatt ctttttaacc aaatgaatgt catagtaata acttgtggaa
    26701 tttaaataat gtgagtatga aaatttttct ttgcatttgt aatatgctcc gaaaccatat
    26761 agttctctga aaagcctttg cacttcaact tcaagcattt cggtgtaggt tcgtgtatca
    26821 acaaattcac gaaatgacca aaagtaatac caccctcttt ttacttcaaa ctccttaata
    26881 aatgtatgat tttgcaattc cttgaaaaca ctattttctc ttagtgcgat ttggaagtca
    26941 tccatattat aattggagac attctctata tgtgtcggaa tttcaagtga aatttcgaac
    27001 ccctttggct taaatgaaga ctgaactatc gaattgctgg ccaacgatgc accgtttaat
    27061 atccatatga gattattttt attataaaat tgttctcgct catatattct tgctatatca
    27121 atacttgaat tttgtatttc aataacttta tccttattgg tttttatatc agcgcgatgc
    27181 caaattgtat tatcgttatt aaatattaca acctcacgat tctctaaagg gaacttgttt
    27241 tgccaatcaa tatgccattg agtcattggc tcataccaag tatcacattc ttttgttttg
    27301 tgataccaat gtggagtgtt aaaatctcct ttgcggccat aaacttttcc tttacaacca
    27361 ggacaatagg ctacgtcgcc agatttgata acttcaatct tttcaccatc ctgattaata
    27421 gcgtatcgca taattcttta accaaaaata acaaaaacat gcaagatttt atccccaaaa
    27481 cttgctactt ctagcaaatt tacccccctt acccattcct ccacaaaatc atagactaat
    27541 ttggaa
//
LOCUS       DAORKW010000004        25612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_7_length_25612_cov_5.264820, whole genome shotgun sequence.
ACCESSION   DAORKW010000004 DAORKW010000000
VERSION     DAORKW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 25612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25612
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7_length_25612_cov_5.264820"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLB46_00410"
     CDS             complement(<1..570)
                     /locus_tag="PLB46_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRF74999.1"
                     /translation="MKISVVGTGYVGLVTGTCFAETGNHVICVDIDAAKVSRMQAGEI
                     PIYEPGLETLFHRNIKQGNLEFTTDLKAAVEQTDIVFLALPTPPNEDGSADLKYVLKV
                     AEDLGGIINGYKIIIDKSTVPVGTADLVRERIAATCKHEFDVVSNPEFLREGMAVEDF
                     MKPERVVIGTESEKAKDMLNRLYAPFVRQG"
     gene            786..1847
                     /locus_tag="PLB46_00415"
     CDS             786..1847
                     /locus_tag="PLB46_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRF75000.1"
                     /translation="MLKPLPVPFDLETKQVLKKLPAAHAALAELKVLAETLPDPNILI
                     NTLGLQEAKDSSAVENIITTHDELYKSELLFEDYKSLHAKEVQNYIAATKTGYHLLQQ
                     TGMLTNRTILQIQEILEANRAGYRKLPGTKIKNTHTGEIIYETPQDPDTIIALMSNLE
                     QFINDNEISDYDPLIKMAIIHYQFESIHPFYDGNGRTGRIINLLYLILQNLQTMPILY
                     LSNFIMNNKTRYYHLLKQVHNQQTWEEWIVFMITCVETTARETIEKIREIKKLMLQTK
                     LQIKNNYKFYSPELIHNLFKHPYTKIEFVVHDVGVTRLTAANYLNKLAADKILIKHKL
                     GTGNYYINHQLYDLLTNGK"
     gene            2034..2609
                     /locus_tag="PLB46_00420"
     CDS             2034..2609
                     /locus_tag="PLB46_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRF75001.1"
                     /translation="MRKLKLQVQTTIDGFMAGTSGELDWAATEWSDDLKNFVIGLTGP
                     VDTILLGKNLAMGFIPYWTAALKEPVPAEGAKIFVETPKIVFSSTLHANDPVAQDWTN
                     TSIENGDYIEKINALKNETGGDIIVYGGGKFVASLLKANLIDELNLFINPVAIGKGMP
                     IFCAIESQQKYALESAKQFVCGITVLTYTKL"
     gene            2611..2931
                     /locus_tag="PLB46_00425"
     CDS             2611..2931
                     /locus_tag="PLB46_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF75002.1"
                     /translation="MRRDVFQAIADPTRRAIITLIAVHALTPNAIAAHFDTSRQAVSK
                     HLRILTECELVEFTENGREIYYHLNINKMKEIDKWLEQFRKIWEGRFNQLDNVLASLK
                     KNKK"
     gene            2933..3898
                     /locus_tag="PLB46_00430"
     CDS             2933..3898
                     /locus_tag="PLB46_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF75003.1"
                     /translation="MNNNLHFDFSVDKQNSTIHVVREFAADLELVWAAWTTPEILDLW
                     WAPKPYQTKTKSMNFTVGGSWLYAMMGPTGDTHWCRADYKSIDPLKSYSGLDGFCDEE
                     GNLNTEFPRSLWHNTFVVKGNNTAVNIQIKYDSLEDLEKVIALGFKEGFTMALGNLDQ
                     YMEQQFKLSNMMKTNNTSRVSTYLNFPGNTEEAMLFYQSVFKSEFTAPGIQRFGSLPA
                     DPNQPPIDDSVKNMVLHVELPIMGNFILMATDAPKEMGFNLIEGNNMHIQLEPDTKEE
                     AIRLFNELSEGGKISMELQDMFWGALFGSFTDKYGINWMINHLKK"
     gene            complement(3995..5065)
                     /gene="prfA"
                     /locus_tag="PLB46_00435"
     CDS             complement(3995..5065)
                     /gene="prfA"
                     /locus_tag="PLB46_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRF75004.1"
                     /translation="MDFVSKLQAIKEKWMNIQEQLSDPEIIANMKRFKTLNKNYRDMQ
                     PVVEAFDKYQLVLGNLQDSRQILNTEKDDELREMAKSELIQFEAEKTRLEEEIKLLLI
                     PKDPEDEKNAIIEIRAGTGGDEAAIFAGDLSEMYIRFCNKQGWKVEVMNENQSEKGGY
                     KEIVLEVTGENVYGKLKYESGVHRVQRVPATESQGRVHTSAATVAVLPEAEEFDIEIS
                     VNDIKVDVFRASGAGGQHVNRTESAVRMTHIPTGIVAECQDERSQIKNREKAMKVLRT
                     RVYEKFHNEHLAKLSQQRKTLVSTGDRSAKIRTYNFPQSRITDHRINMTLYNLPEAMN
                     GYIDEILDALQVAENAEKMREG"
     gene            complement(5250..6041)
                     /locus_tag="PLB46_00440"
     CDS             complement(5250..6041)
                     /locus_tag="PLB46_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75005.1"
                     /translation="MKNKYLIILLLLTHTVYAVDSVVCKISVTASYFTADALSDIYYI
                     NQNNEIVKTHCGVGDLFTYSNKNLGRPVSIDASNPMKVLVFYPDQQTIVILNNQLAEL
                     SALQLRGNINVQNFQPSAICKHTGTDHIWMYDELSRKLYRLDESGNQLAMSEAFDQLF
                     TEAIEVETLFSANDKVFISSKNSGVLVFDAYANFEKIIPTTFNVQQITDNYIIGVKEN
                     LLQFFEFGSQEIIDYPITGTNARLIGKSVFVQNQDGITIFDLGEH"
     gene            complement(6031..6774)
                     /locus_tag="PLB46_00445"
     CDS             complement(6031..6774)
                     /locus_tag="PLB46_00445"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRF75006.1"
                     /translation="MERNKIYFASDFHLGIPDYKSSLEREKKIVRWLESIENDAAKIF
                     LVGDLFDFWFEYKYVVPRGHVRLLGKLAQLKDKGIDIQIFTGNHDLWMYGYFEEELQI
                     PVHHKAIQFEFSGKRFFVGHGDGIGPGDYGFKFMKRVFKSRLAQILYGSLHPYIAFKV
                     ARYFSYNSRRMNEEEKFMGEDKEWQIIFAKEVLQKEHFDYFLFGHRHIVLDIPIGERS
                     TFINLGDWIIHFTYAEFDGNTCKIKYFEK"
     gene            complement(6761..7432)
                     /locus_tag="PLB46_00450"
     CDS             complement(6761..7432)
                     /locus_tag="PLB46_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRF75007.1"
                     /translation="MAKILSSKEKMLSSVRAALYHQNKQPYPNLESNDAIYALENEPL
                     EIIFASEFTEVSGQFVYCENEKECVDTLQELVTQHGWTHISCPEVVLHEMFDRFQYIN
                     YETDLAVTSTDAGIMLCEALIARTGSILISSRQASGRQLPVFPEVNIVIASTRQLVMD
                     INEGFAAIRKKYDGNFPSMVNLNTGPSRTADIEKTLVLGAHGPREVYVFLIENWPVAG
                     WDGEK"
     gene            complement(8313..10283)
                     /gene="ftsH"
                     /locus_tag="PLB46_00455"
     CDS             complement(8313..10283)
                     /gene="ftsH"
                     /locus_tag="PLB46_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRF75008.1"
                     /translation="MSTEQNNQNKNKPGAPKKFNFYWIYGLIAVILIGFQFFPFQGKP
                     VELKNIDKFKTEMLQQGDVNRVVVINKEFAEIYIKEDKLTEEPYKTDKVPEKGPQYTW
                     TIGSTETFERQLAEWQQGGADKPITVSYETRHNVLLNLLSYLLPIILILAIWIFIMRR
                     VTGGGGMGGGGQIFNIGKSKANLYDKDTKVNVTFNDVAGLDEAKEEVMEVVDFLKNPK
                     KYTSLGGKIPKGVLLVGSPGTGKTLLAKAMAGEAQVPFFSISGSDFVEMFVGVGASRV
                     RDLFRQAREKAPCVIFIDEIDAIGRARGKNMIQSNDERENTLNQLLVEMDGFGTDKGV
                     IILAATNRPDVLDPALLRPGRFDRQISIDKPDLAGREQIFKVHLKPIKVAPDLDAHKL
                     ALQTPGFAGADIANVCNEAALIAARRGKKFVEMQDFNDAVDRVIGGLEKKNKLISQDE
                     KKIVAYHEAGHAICGWYLEHADPLVKVSIVPRGIAALGYAQYLPKEQYLYTTEQLTDM
                     MCMTLGGRAAEDIIFGRISTGAQNDLERITKMAYAMITIYGMNERIGNISFNDPQQDY
                     RFSKPYSEQMAQAIDEEVKKMIANAYNRTKVLLNEKKPQLEIIAKELLDKEIIFKADM
                     ERLIGQRPFEEKHAEVEVKADAIVFEEEKTAE"
     gene            complement(10302..10676)
                     /gene="rsfS"
                     /locus_tag="PLB46_00460"
     CDS             complement(10302..10676)
                     /gene="rsfS"
                     /locus_tag="PLB46_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRF75009.1"
                     /translation="MRKKTKQSTTTQYSLTDLVIESILDKKGEEVVHLDLTALNDAVA
                     DHFIICHADSTTQVMAIGNHIAQNVLDKAGLRPFGKEGFENAEWIIIDYTDVVVHVFY
                     KEKRYFYQLEDLWHDAAIKKVS"
     gene            10770..11477
                     /locus_tag="PLB46_00465"
     CDS             10770..11477
                     /locus_tag="PLB46_00465"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRF75010.1"
                     /translation="MESTNAYMRQVLDSSSISIPEGLLITTDEQFSGKGQGAKTWLSE
                     PKQNLTLSILLRPVFLPPRYVFYLNKAVALAVHDTLATEMHGIAIKWPNDLYHHNNKL
                     GGILIENVIDSTAIRQSIIGIGINVNQTVFDASIPNPTSISLITGRETDLSALLEKLC
                     GQLEKYYLQLRASQFTAIDALYHHYLFGHQAQHEFLINNEKIIATVNGVNPEGKLILS
                     TPDSKLTLAVGEVSWRL"
     gene            11795..12916
                     /locus_tag="PLB46_00470"
     CDS             11795..12916
                     /locus_tag="PLB46_00470"
                     /inference="COORDINATES: protein motif:HMM:NF023021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75011.1"
                     /translation="MTCRGQFQDPKADAVVYNTLASANKWKHLFSIVFIVFIWGNADY
                     VFAQGAVKYSITYVTESKTTYQTNDSLNTFTIEEQLQLMPVTTRDSIIKLVYTNNDIK
                     TTIYHQVNTGFPEWATKPAKTIIDKTRIKVYDDHGALMINQAHSAKFKSKNNQLKSFL
                     TTNSFDIIPDYVYLSPTMKTDFTARGFEMTNLGDGTFRFVKDSVTLYYNNSRRTNELV
                     LKAEDGTTRLGIKRAFQTNVRGVIVPAFEIKTKKADSFAGHCVNEVAYTNYVYYDYTT
                     FAGGKWEDEDESSVDDEPLELQVSPNPANTIITIQIPIAANTQTLNLYDNTGKMVYQS
                     VIEAGEFEIQIDITHLESGIYYAQLQHSEALITTSFIKN"
     gene            12935..14464
                     /locus_tag="PLB46_00475"
     CDS             12935..14464
                     /locus_tag="PLB46_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75012.1"
                     /translation="MKKIISLYILLLIGIHLNAQLALDCIDLNKSITITPAIPFDYIP
                     SLFTDDIWATPTFITDRPVYFIHGLGGQGDEAGTVGISWSQASLWSEENYYINSRRPD
                     YADVSLDFAATELKGDLENYLSGSPDAFLIAHSQGGIVSRRVDKMYSLHELGYEPRTF
                     GGLVTFGTPHQGAMILNNRDELLAWTGTTCEKLSDGPVTELVEANFFLDLALSPVGID
                     TFEKKFCDILEFNIAPFLFKDYFSGITESYMVGSEQLAELNAFVPEIPYVCFYGIETE
                     PVLWNTLTHIFPGHEPNNVITYGEEPFGSGGDQMLVNYADAMMDKYWTKYYAYDYLAD
                     IYSDLIYGFDLTTIICYMTALCYFSAHNSLEECEDISAAYYKGFTWFTDANEVWEGLI
                     GAGSLQPESETCYCIDWGPLGDVNDDEYEIEPGTSCYTDDANTDCTTTTNYAWVVKPN
                     DGVVLAESASECVGNVLSFIGKAMYGSNHFSMRNDINTQFRLIELYNGLHGEYFINDE
                     R"
     gene            14451..15722
                     /locus_tag="PLB46_00480"
     CDS             14451..15722
                     /locus_tag="PLB46_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRF75013.1"
                     /translation="MMKDKMQKGYLVILKLFLLVYACKQEEIISKNGDQNVEDPSSKY
                     VEFLWKFPLAMDTSERVSMTPVLSNGNVVFTAKILLTPDPARVKCIDTAYRTIVWESD
                     DVFMEDCIEISMVDGSGHGVYLNYFATLCDVNPRVIDLTNGDILWDYNGIGGDGHMCL
                     WNDKFFHSNVTGINPFTHSEIVMSDILTQNFRTVYAVDMESGYSCHIYPPAVMVNDES
                     DTLLFFQNRQWKSPPYDGKIDFYGYNMSDDILLWKLDDINESGNSAVFIPLIHDNKVY
                     FKAGFEVFCFDAETGNIIWEFNPPSVSDMLLGNLLIEEGKLIVKTSGPELYAISLETG
                     NIIWENLNAGETPYSLTYFEGNIYYVAGGDGKLHVIEVATGKEFWSILTPNQSESVSF
                     GTTFFNGVAIDPATRRLYITDTHFINCFQLK"
     gene            15729..16439
                     /locus_tag="PLB46_00485"
     CDS             15729..16439
                     /locus_tag="PLB46_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75014.1"
                     /translation="MKNCIVLSILLSLLTTARAQENTGPTHHFGISLGANYLFPFDND
                     LNIGVKETGFNGSATYTLQQGKIGIQLSPFAKLHSGEDFDAMKQYYKDKGVTLNSYNS
                     GSYLSTGMDAGLQFILVDHGKLPVIKTYIQYGISSVRVPEINFTSESEQDGNVNVRAD
                     GGLGLGSNLQFGVVCDVLKSNNTIYNVKLGFMQETANVAVPTSTNVESQSETIRSVEM
                     FEWRSFSAFLNLSVFFDL"
     gene            complement(16755..19109)
                     /locus_tag="PLB46_00490"
     CDS             complement(16755..19109)
                     /locus_tag="PLB46_00490"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF75015.1"
                     /translation="MKFLHLILMQIISTIIAQAQALEVEWEKSFGGIIYEEAFDIIAT
                     ADSNFLVVGRGGPLAADFFDCGEYTGFVIVKITSAGEILWSKCYGGNEYSLAHAIINS
                     SEGGYVVAGETYASDGDITGAHGNSDFWVIKISDTGELEWQLAVGGTSHDRASDIIQS
                     NDGSYLVVGDSRSINGDINDHHGTESTSDLLLAKINIGGELIWTNSYGGIYDDTGYSI
                     VLDDSENLILIGYSASTGGDVPENKGSTDLWVLKVNTDGEIIWSKTFGGSETDIGYSM
                     SIMENSIFLVGTTFSNNFDVSGNHGFQDGWVLKLNQDGDFISQKCFGGSNFEIFDDIY
                     TINSSTILLTGASGSINGDLTEHFGAIGYADWWAVKVDTNLNLVFQKSLGGSLNDRAY
                     AGLFLDENQFIITGTSYSSDGYVSENGGGSDMWTVKLNVCYDKYFADTDGDGFGDLTD
                     DSISCNMPAGYVFDSTDCNDLNPDIHPTLTDICNSIDDNCNGLFDEDATFTTYYLDAD
                     ADLFGATGTDSISCSVVVGYVENSFDCNDLNAEINPLIIETCNGIDDNCNVAIDEGLT
                     LYTFFADVDGDTYGNPDAGIDTCLETVFGYVSNNLDCNDTLATIYPGATELCNYLDDD
                     CDGLVDDNVAFIQSYADIDSDNFGNILIDSISCELPEGYVLDNTDCDDTNPDIYPGAT
                     ELLNGLDDDCDQVADEGLSIENIKNIICSISLNPSFDVINITSNILGSGEFEFVSSIG
                     QILFTGEWDANNNSISIIKLPAGVYTIRLSFNNTVASLPFIKIN"
     gene            19226..19936
                     /locus_tag="PLB46_00495"
     CDS             19226..19936
                     /locus_tag="PLB46_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75016.1"
                     /translation="MKIKHAMYVLLILSAILSMQTLAYPTEPIKFSYENYLTSLIESE
                     NFALPDTSVQIEEGDTIAKYTAGGDSVYVLYEDSIPDSSFNMAWEIPFPTEHMIIELT
                     NHHLPVEEGLFGINVTDMFQTKFANEDELPTIYGTNPDPWDYLSQLQPKILRFPSGAG
                     GKFYQPLGSIRTNPDDPDVGFINGGYGINIDEVIKFYDATATGVGDAPFLFDPPGDPG
                     YISITDDMGDNNCDNCIL"
     gene            19937..22159
                     /locus_tag="PLB46_00500"
     CDS             19937..22159
                     /locus_tag="PLB46_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75017.1"
                     /translation="MDDSHTSTFEDNYTKWDDQPIIDPTGIVEPSDMGPDLYVNQFIR
                     LVEKIELENSLLLGYTYVVDVVVCLNISTQTATEAVELIDYLLESGINVVAVEIGNEV
                     YFDWGRQMMGFTDHLNPPLGPTSDVSPFIHYWDYINGFEYTDGDSGVDPLDGEYGDED
                     VDLDLASVLPTDVLLDHDYITAINTAFPDIKIGLPGENFNQCEEVGWDTPFIVDDPDD
                     ERLFLPGGPEPECNCDYSDWNIDMETKFDEEIGAGESAGPKFDAIILHTYYNAGNAYV
                     DCPDNTNWQVAATEPTFVGGTPFEDQWTNSPFDPNIGTLFEGIAGIATNPDGVGVPEY
                     QIGNFKEFTRDRIKYAYDQHAINLNFLDGAPGSTKEIWITEYNISPKTSGLTAEDDES
                     FTNMVPNTFAHCVALQNWMLWHVKSYYDSDYKPGIVTMATTQAFIGGNAIDYMTPSNR
                     NDQVELETPNPELTCTSIEYSDVENYFVRRASYFTAQLLNNIHTLDLNYEKTFIGMYS
                     QNNNIAPTTFVQLDNDGEPINVYVFYTNIKQTTQRYAIDPADIYLSIEDAGGVELGAA
                     TVYALNPSQLYSTSGRNQMFYEPINEAYGCAPVLPDSPYVFEITDTWTGLSGIGCPGG
                     FTPPPGGVCVTVPATSAGYFVIPIMPYERKGEIASTFRLYPNPADDFFVIEKTSTNEN
                     VSDDFTMRIYSTFGTLVIESSIRSGEMVNTDILSSGMYTVVIIDSNNNASTLKFSKMQ
                     "
     gene            complement(22314..23267)
                     /gene="metF"
                     /locus_tag="PLB46_00505"
     CDS             complement(22314..23267)
                     /gene="metF"
                     /locus_tag="PLB46_00505"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRF75018.1"
                     /translation="MKVTEHLAKAEKTLLSLEILPPLKGKSIKGIYDVLDHLIEFNPS
                     FINVTYHRSEYIYKKRGDDRFDKVFIRKRPGTVGICAAIQFKYKIDAVAHIICGGFTK
                     EETEDALIDLSYLSIDNVLLLRGDASKNERDFIAEPGGHKYASELVKQVVNMNNGIYL
                     EEDSIQGYKTDFNIGVAGYPEKHFESPNLKSDLRFLKQKVESGADYIVTQMFYDNQKY
                     FDFVKKCREEGINIPIIPGIKPITAASQIHSIPRTFHVDIPEALSDEIDNCKTPEAVK
                     AAGVKWCIQQSQELVAAGAPCIHYYTMGNVDAMRTIVDEVF"
     gene            23422..24696
                     /gene="purD"
                     /locus_tag="PLB46_00510"
     CDS             23422..24696
                     /gene="purD"
                     /locus_tag="PLB46_00510"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRF75019.1"
                     /translation="MNILLLGSGGREHALAWKIAQSPDCTKLYIAPGNPGTAELGTNV
                     PIKPTDFDALKSFCLNNQIQMVVVGPEDPLVHGVVDFFEQDAAVKHIPVIGPNKIAAQ
                     MEGSKTFAKAFMLRNNVQTPAYREFNQSNTEEAIQYVKQHSMPVVLKADGLAAGKGVV
                     ISNTPDEAANELQLMMGGKFGDAGNKVVIEQFLHGIELTVIVLTDGNSYMILPPSKDY
                     KRIGDGDTGLNTGGMGAVSPPPFATNDFMQKVKSKIIEPTLDGLKKENIHYKGFLYFG
                     LFNCNNEPYVIEYNARMGDPETQVIMPRIKNNIVELFTAVANGTLHTKTLITDERACT
                     TVILASHGYPETFEKGFPITGLENTENCMVFQAGTGLGNNGELVTSGGRVMAVSAYGN
                     DLAEALRNCYINAGRIYYDSRYFRRDIGFDVM"
     gene            24931..25335
                     /locus_tag="PLB46_00515"
     CDS             24931..25335
                     /locus_tag="PLB46_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75020.1"
                     /translation="MKTFLFAAITAILFASCSKQTNCIGSDCVLPEAAIAIDSVTNNG
                     IFVRLPNNEGGFFACTLELASHINQDEFLAAVSAYTTASPVPGSETQTMPMLKVYYVA
                     EDQSYVRENARYIHASNNKLEIISYGNNLETD"
BASE COUNT         8061 a         4984 c         4463 g         8104 t
ORIGIN      
        1 gccctggcgt acaaatggtg catataagcg attgagcatg tcttttgcct tttcactttc
       61 agtgccaatt accacgcgtt cgggtttcat aaaatcttcc acagccatgc cttcgcgcaa
      121 aaactccgga ttgctaacca catcaaactc atgtttacaa gtagcagcaa ttcgctcgcg
      181 aaccaggtca gcagtgccaa ccggaacggt gcttttatcg ataatgattt tatatccgtt
      241 gataatacca cctaaatcct ctgccacctt taacacatac ttcaaatcgg cactgccgtc
      301 ctcatttgga ggggttggca aggccagaaa tacgatatcg gtttgttcaa ccgctgcttt
      361 taaatcggtc gtaaactcga gattgccttg tttgatattg cggtgaaata aggtttctaa
      421 ccctggttca tagatgggaa tttctcccgc ctgcatccgt gaaaccttag cagcatctat
      481 gtctacgcaa ataacatgat tgcctgtttc ggcaaaacaa gtgcctgtta ctaatccaac
      541 ataacctgtc ccaacaactg agattttcat ggggcaaaga taagcagatg ttatggatta
      601 tgcactcgca accacgcaag ctgccatttg cctaaataag gtttttatac atagttgaat
      661 tgtaatgtat agattttatg attttttata cataaaggct cttaaatgta taaattttac
      721 tgttttttat acttaatctt tatatttgta tagaaaattc aattttctat acaaaagaat
      781 cgattatgct gaaaccactt ccggttcctt ttgacctcga gacaaaacag gtgctgaaaa
      841 agctgcctgc tgcccatgcc gccctggctg aacttaaagt gctggctgaa actttacccg
      901 accccaatat tttaattaat acactgggat tgcaggaagc aaaagatagt tcagcagtcg
      961 aaaatattat tacaacacat gacgagttat ataaatcgga attgttattc gaagattata
     1021 aatcattaca tgcaaaagaa gtccagaatt atattgctgc tacaaaaacc ggatatcatt
     1081 tgttacagca gacaggtatg ctaaccaatc ggacaatttt acaaattcag gaaatattgg
     1141 aagcaaatag agcgggttat cggaaattgc ctggtacgaa aattaaaaat acacacaccg
     1201 gtgaaataat ctatgaaaca ccacaggatc ccgatacaat tattgcgctc atgtctaatt
     1261 tggagcaatt tataaatgat aatgaaatta gtgattacga cccacttatt aaaatggcaa
     1321 ttatacatta tcaatttgaa agtatacacc ctttttatga tggcaatggc cgcacaggtc
     1381 gcataattaa tttgttatat ctcattttac aaaacctgca aacaatgccc atactgtatt
     1441 tgagcaattt tataatgaat aataaaacca gatattacca tttattaaaa caggttcata
     1501 atcaacaaac atgggaagaa tggattgttt ttatgattac ttgtgttgaa actactgcgc
     1561 gcgaaacaat cgaaaaaata agagaaatta aaaaattaat gttgcaaacg aaactacaaa
     1621 ttaagaataa ctacaaattt tatagtcctg aattaatcca taatttattt aaacatcctt
     1681 acacaaaaat tgaatttgta gtacatgatg ttggtgtaac caggttaacg gctgcaaatt
     1741 atctgaataa actggcggct gataaaattt taattaaaca taaacttggt accggaaatt
     1801 attatatcaa tcaccaattg tacgacttgc tcacaaatgg aaagtaaata atgcgtttag
     1861 cttgatgcat ttccattagt ctaatttttg tatacactta atacttaatc ggaatacatt
     1921 tacctaggcc taaaactgat ttttttaaat tttaactaaa aaaattttga gaaaccaatc
     1981 ggttgcgtat atatttgcaa ccaattagtt gcataactca aaacaaaaaa caaatgagaa
     2041 aacttaaact tcaagtgcaa accaccatag atggcttcat ggccgggact tccggcgaat
     2101 tggactgggc ggcgacagag tggagcgatg accttaaaaa cttcgtcatc gggcttaccg
     2161 gtccggttga cactatttta ttaggcaaaa acctcgcaat gggtttcatt ccgtattgga
     2221 ccgcggcatt aaaagaaccg gtaccggcgg aaggtgcaaa aatttttgtt gaaacaccca
     2281 aaattgtgtt ttcatcaaca cttcatgcaa acgacccggt cgcccaagac tggacaaata
     2341 ccagcattga aaatggtgat tatattgaaa aaataaatgc acttaaaaat gaaacaggtg
     2401 gcgatattat tgtttacggc ggaggtaaat ttgttgcctc tcttttaaaa gcgaatttaa
     2461 ttgatgaatt gaatttattt atcaatccgg ttgccatcgg aaaaggaatg ccaatttttt
     2521 gtgcaatcga aagtcaacaa aaatacgcgc tcgaaagtgc aaaacaattt gtttgcggta
     2581 ttactgtact tacttatact aaattataat atgcgacgag atgttttcca ggctatagcc
     2641 gatccaacca gaagagcaat aattactctg atagccgtac atgcgctaac accaaatgct
     2701 attgctgcac atttcgatac cagcagacaa gcagtatcaa aacatttgcg cattcttacg
     2761 gaatgtgaat tggttgaatt tacagaaaat ggtagagaaa tttattatca cctcaatatc
     2821 aataaaatga aagaaataga taaatggctc gaacaattcc gcaaaatttg ggaaggccgc
     2881 tttaatcaat tagacaatgt tttagcatca ctcaaaaaaa ataaaaaata aaatgaacaa
     2941 caacttacac ttcgacttct ctgtcgacaa acaaaatagt accatacatg tcgttagaga
     3001 atttgcagca gacctcgaat tggtttgggc tgcatggact actcctgaaa ttttagattt
     3061 atggtgggcg ccaaaaccgt atcagacaaa aactaaatcg atgaatttta cggtaggtgg
     3121 cagctggctt tatgcaatga tgggaccgac aggtgatacg cattggtgtc gtgccgatta
     3181 taaaagcatc gacccattaa aaagttattc agggttggat ggtttttgcg acgaggaagg
     3241 caatttaaat accgaattcc cacgctcgtt atggcataat acatttgtgg taaaaggaaa
     3301 taatactgca gtaaatattc agattaaata tgattcactt gaagatttgg aaaaagttat
     3361 agccctcgga tttaaagaag ggttcaccat ggcattaggt aacctcgacc aatacatgga
     3421 gcaacaattt aaactcagta atatgatgaa gactaataat acttctcgtg tttccacgta
     3481 tttaaatttt cctgggaata ctgaagaggc catgttgttt tatcaatcag tatttaaatc
     3541 agaattcact gctccgggca ttcaacgttt tggtagttta cctgccgacc cgaaccaacc
     3601 accaattgac gattcggtaa aaaatatggt gttgcatgtt gaattgccaa taatggggaa
     3661 ttttattctg atggcaacag atgcgcccaa agaaatgggt ttcaacttaa ttgaaggtaa
     3721 taacatgcat attcaactcg agccggatac caaagaagag gctatacgat tatttaatga
     3781 attatcggaa ggaggaaaaa tatctatgga attacaagat atgttttggg gagccttatt
     3841 tggttcattt accgataaat acggcattaa ctggatgata aatcatctca aaaagtaatt
     3901 caacagtaat atactaaaaa agcacgaata aaattcgtgc tttttttatg tcgtaaaata
     3961 tcaatttata attacaaaat tatttcccca agatttaacc ttctctcatt ttctccgcat
     4021 tttctgcaac ctgcaaggca tctaaaatct catcaatata tccattcata gcctctggta
     4081 aattatataa ggtcatatta atacgatgat cggtaatcct gctttgcgga aaattatatg
     4141 ttctgatttt tgccgaacgg tcgccggttg agactaatgt tttgcgttgt tgcgataact
     4201 tggcaagatg ttcattatga aatttttcat atacacgagt acgcaacact ttcatcgctt
     4261 tttctcgatt ttttatttgg gaacgttcgt cctggcattc agcaacaata cctgttggaa
     4321 tatgcgtcat gcggactgca ctttcagttc tgtttacgtg ctgcccaccc gcaccactcg
     4381 cacgaaatac atctacttta atatcattta cacttatttc aatatcaaat tcttctgctt
     4441 caggtaaaac tgcaacggta gcagcactcg tatgcacgcg cccctgactt tcagttgccg
     4501 gtactctttg cacacgatgc acaccacttt cgtattttaa tttgccataa acattttcgc
     4561 cggttacttc taacactatt tctttgtaac cacctttttc actttgattt tcattcatta
     4621 cctcaacctt ccatccttgt ttattacaaa aacggatata catctcactt aaatctccgg
     4681 caaaaatagc agcttcatca ccacctgttc cagcgcgaat ttcaatgatg gcattttttt
     4741 catcttcagg gtcttttgga attaatagta attttatttc ctcctcgagc cttgtttttt
     4801 ctgcttcaaa ttgtattaat tccgacttcg ccatttcacg taactcatca tccttttcgg
     4861 tatttaatat ttgtctgctg tcctgcaaat ttcccaacac caactgatat ttatcgaatg
     4921 cttccaccac cggctgcata tcgcgatagt ttttattcaa cgttttaaaa cgtttcatat
     4981 ttgcaataat ttccgggtca ctcagctgct cctgaatgtt catccacttt tcttttattg
     5041 cctgtaattt tgaaacaaaa tccattgtga taaattcgac ttaaaattat aatttattcc
     5101 tgatacctaa attgtgtttt tatcctttta ctttcataac cttttcattt accctacttg
     5161 ccttgaaata gatgaatacc aatcacacga tacctgctta taaaattata gtgaaatgtt
     5221 ctggcaattt aggtgtaaag gcagcgcacc taatgttcgc ctaaatcaaa aatagttata
     5281 ccatcctgat tttggacaaa aacagatttt ccaattaatc gtgcattggt gccggtaatt
     5341 gggtagtcaa taatttcctg actaccgaat tcaaaaaatt gaagtaaatt ttctttaact
     5401 cctatgatgt aattatctgt aatttgctgg acattaaatg tagtaggaat aattttttcg
     5461 aaatttgcat aggcatcaaa tactaaaaca ccactatttt tgctgctgat aaatacctta
     5521 tcatttgcac taaaaagtgt ttccacttca atagcttcag taaacaattg gtcaaatgcc
     5581 tcactcatgg ctaattgatt tccgctctca tctaaacgat ataattttct cgataattca
     5641 tcatacatcc aaatatggtc ggtgccggta tgtttacaaa tagctgaagg ctgaaaattt
     5701 tgaacgttaa tatttcctct aagttgtaat gcggataatt cggcaagctg gttatttaaa
     5761 ataacaatag tttgttggtc gggataaaac accaatacct tcattgggtt agatgcgtcg
     5821 attgaaaccg gacgacctaa atttttattg ctataagtaa ataaatcgcc aactccacaa
     5881 tgggttttaa caatctcatt attctgattg atgtaataaa tatccgataa agcatctgca
     5941 gtgaaataac tggcagtcac acttatttta caaaccacac tatcaacagc ataaactgtg
     6001 tgagttagta gcaataatat tatcaggtat ttatttttca aaatatttta tcttacaggt
     6061 attgccatca aactcggcat aagtaaagtg aattatccag tcgcccagat taataaacgt
     6121 tgacctttct ccaattggta tatctaaaac aatatgtcgg tggccaaata agaaataatc
     6181 aaaatgttct ttttgtagca cttcttttgc aaaaataatt tgccattcct tatcctcacc
     6241 cataaatttt tcttcttcat tcattctgcg gctattgtaa ctgaaataac gcgctacttt
     6301 gaaagctatg taagggtgca aactaccata tagtatttga gctaagcgac ttttaaatac
     6361 ccgtttcatg aatttaaatc cataatcacc tggtccaata ccatcgccat gtccaacaaa
     6421 aaaacgtttc ccgctaaatt caaattgaat agccttgtga tgaacaggaa tttgtagttc
     6481 ttcttcaaaa tatccgtaca tccacaaatc gtgattgcct gtaaatattt gaatatcgat
     6541 acctttatct tttaactgtg ctaatttacc aagcaaccga acatgcccgc gaggtactac
     6601 atatttatat tcaaaccaaa aatcaaataa gtcgccaacc agaaaaattt ttgcagcatc
     6661 gttttcgata ctttcgagcc agcgtacgat ttttttttcg cgttccagac tggacttata
     6721 atcgggaata cctaaatgaa aatcagaagc aaagtaaatt ttatttctct ccatcccatc
     6781 cggcaaccgg ccagttttcg attaaaaata catatacttc tcttggacca tgagcaccta
     6841 atacaagtgt tttttcaata tctgcagtgc ggcttggtcc ggtatttaaa tttaccatac
     6901 tcggaaaatt gccatcatat ttttttcgta ttgctgcaaa tccttcgtta atatccataa
     6961 ctaattgtcg ggtagatgca attacaatat ttacttcagg aaaaaccggt aattgtcttc
     7021 cacttgcttg tctgctcgaa attaaaatgc tgcctgttct tgcaattaat gcttcacata
     7081 acataattcc cgcatctgta gaagttacag ctaaatcagt ttcgtaatta atgtattgaa
     7141 atctgtcaaa catttcgtgc aacacaacct cgggacaact aatatgtgtc caaccgtgtt
     7201 gagttactaa ttcttgtaag gtatccacac attccttttc attttcacag tacacaaact
     7261 ggccggatac ttctgtaaat tcagaggcaa aaatgatttc cagaggttcg ttttccaatg
     7321 cataaattgc atcattcgac tccagattcg gatagggttg tttattttga tgatacaatg
     7381 ctgcccgaac actgctcagc attttttctt tcgatgataa tatttttgcc atttacctgt
     7441 ttgtgttaat caataatctt ccagaatccc caaatttagg gcttttacag taattcccca
     7501 tatcaaacca tgttggttct tcccacccca acctccaaag tgtccacaaa tcctcaataa
     7561 cccaccccaa cccccatcac ctagctgttt ccgcctcaga ggacgcttcg cgtattggta
     7621 catatatcag cttcggtatg aaattatcat caggtcaaca cgtgggttga ccacaacagt
     7681 aactccgaat tccattccaa ctccaatccc ggctgttaat cagcacataa tcacatcagc
     7741 acataaccac atcatccggc tgtttcattg gtacatcggt acatcattac atcggtacat
     7801 tagttgtttc tcgcaaagct gcaaagggta tggttcatac aatgtgttaa ttcaagtgcg
     7861 caaagcttat aaaccaggtt cacgcggaaa aagggaaaaa gtcggagaat agtatcggct
     7921 gtttaattaa cacatcagca catcacccgg ctgttcccgc ctcggcggac ggttcgcatt
     7981 ggtacattcc cgcattggta catcggtaca ttaatcagca catcagcaca tcagcacatc
     8041 agcacatcag cacatcagca catcacccgg ctgttcccgc ctcagcggac ggttcgcatt
     8101 ggttcattcc cgcattggta cattcccgca ttggtacatc ggtacattaa tcagcacatc
     8161 agcacatcag cacatcacca catcagcaca tcaccacatc agcacatcat ccggctgttc
     8221 cattggtaca tcggtacatc cccacattgg tacattaaaa aagccgatgc atcatctaca
     8281 tcggcttttt tctcaaaaat tttaatgcag aattactccg cagttttttc ttcttcaaac
     8341 acgatagcat ctgcttttac ttcaacttca gcgtgttttt cctcaaaagg tctttgacca
     8401 ataaggcgtt ccatgtctgc tttgaaaata atttctttat ctaaaagttc tttagcaata
     8461 atttccaatt gaggtttttt ctcattcaac aacactttcg tgcggttgta agcattggca
     8521 atcattttct taacctcttc atctatcgcc tgagccattt gttcactata aggttttgag
     8581 aaacggtaat cctgctgagg gtcattaaaa ctgatattac caatgcgttc attcatacca
     8641 taaatggtaa tcattgcata cgccatttta gtaatacgtt caaggtcatt ctgagcacct
     8701 gttgaaattc gaccaaaaat gatatcctct gctgcgcgac cacctaaggt catacacatc
     8761 atatcagtaa gttgctctgt agtatacagg tattgttctt tcggtaaata ctgtgcatat
     8821 cctaacgcgg caattcctct tggtacaata gatactttca ccagtgggtc agcatgttcc
     8881 agataccagc cgcaaatagc atgaccggcc tcatggtagg caactatttt tttctcatcc
     8941 tgagaaatca gtttattttt cttttctaaa ccaccaatta cacggtcaac ggcatcgtta
     9001 aaatcttgca tttcaacgaa tttttttccg cggcgcgctg ctattaaagc agcttcatta
     9061 catacgttag caatatcagc accggcaaaa cctggtgttt gtaaagctaa tttatgcgca
     9121 tccagatccg gcgctacttt aattggtttt aaatgcacct taaatatttg ttcacgaccg
     9181 gctaaatccg gtttgtcaat agaaatctgt ctgtcgaaac gacccggacg taataaagcc
     9241 ggatccaata catcgggacg gttggtcgcc gctagaatga ttaccccttt gtccgttcca
     9301 aaaccatcca tctcaaccaa taactgattt aacgtgtttt cacgctcatc gttactttga
     9361 atcatgtttt taccacgagc acgtccaatg gcatcaattt catcgataaa aattacacaa
     9421 ggcgcttttt cgcgagcctg acggaataaa tcacgaacac ggctggcacc tactcccaca
     9481 aacatttcaa cgaaatcaga accggaaata ctgaaaaatg gcacctgcgc ttcccctgcc
     9541 atggcttttg ccaacaaggt tttacctgtt cccggtgaac ctactagtaa tacacctttg
     9601 gggattttac cacctaatga agtgtatttt ttagggtttt tcaggaaatc cacaacctcc
     9661 atcacttctt ctttcgcctc atccaaaccg gcaacgtcgt tgaaggttac attcactttg
     9721 gtgtctttat cataaaggtt agctttagat tttccaatat tgaaaatctg accgccaccg
     9781 cccataccac cgcctccggt tactctgcgc atgataaaaa tccaaatagc taaaatcaat
     9841 atgattggta gtaaataact taaaaggttt aacaacacat tatgacgagt ttcataagat
     9901 acggtaatcg gtttatccgc accgccttgc tgccattccg ctaattggcg ctcaaatgtc
     9961 tcggtagaac cgatagtcca agtgtattgc ggaccctttt caggtacttt atcggtttta
    10021 tatggctcct ctgtaagttt gtcttctttg atataaatct cagcaaactc tttgtttata
    10081 actactacac ggtttacatc accctgctgc aacatctctg ttttaaactt gtcgatgttt
    10141 ttcaactcaa ccggtttgcc ctgaaatggg aaaaattgaa atcctatcag aattacggct
    10201 attaaaccat aaatccagta aaaattgaat ttcttaggcg cacccggctt gttcttgttt
    10261 tgattgttct gttcggtact cattacttta aatatacgtt attaactgac tttcttaatt
    10321 gcggcatcgt gccataggtc ttcgagctgg tagaagtatc gtttttcttt gtaaaacacg
    10381 tgcactacta catccgtata gtcaattatt atccattcgg cattttcgaa gccctccttt
    10441 ccaaaaggac gaagacccgc cttatctaac acattttgag cgatatggtt tccaatagcc
    10501 ataacttgtg ttgtagaatc agcgtgacaa atgataaaat gatcggcaac tgcatcattt
    10561 aatgcggtaa gatcaagatg aaccacttct tccccttttt tatccaggat actttcaatg
    10621 acgagatcag ttaaagaata ttgtgtggtt gttgattgct ttgttttttt tctcacttac
    10681 tttgttctga attacaaaat tacgaattct tattgcgccc tccaatcaat acgctattca
    10741 tcggtaaaaa ccaccagcat ctgctaaagg tggaaagtac caacgcgtat atgcgtcagg
    10801 ttctcgactc ctcatcaata agcatacccg aaggactgct cattactact gatgagcaat
    10861 tttcaggcaa aggacaaggc gctaaaacct ggcttagtga gccaaaacaa aacctgacac
    10921 tcagtatttt gctccgaccg gtgtttttac caccccgtta tgttttttat ttaaacaaag
    10981 ctgtcgctct ggcagttcat gacacattgg ctactgaaat gcatggtatt gccataaaat
    11041 ggccaaacga cctgtatcac cataacaaca aattaggtgg cattttaatt gaaaatgtaa
    11101 tagacagtac cgcaatccgt caatctatta ttggaatagg tattaatgtg aatcaaaccg
    11161 tttttgatgc ttccatacct aatccgactt caattagcct cataacaggc agggagactg
    11221 atttatctgc gttgctcgaa aaattatgcg gacaattgga aaaatattat ctccaattgc
    11281 gtgcttcaca atttactgcc atagatgcac tttatcacca ttatttattt ggtcaccagg
    11341 cccaacatga atttttaatt aataatgaaa aaataattgc aacagttaat ggggtaaatc
    11401 cggaaggcaa actcatatta tccactcccg attcaaaatt aacactagct gttggagaag
    11461 taagttggcg actttaataa aataggtaga tgtcttattc gttatttaca taaaaggata
    11521 tttctacaga agtttttttc aattaattgg ccagccaccc cattttgcac ttgatttatt
    11581 atcgccccca atcgtttaat cgaataattt tttttatgaa ttatgcaaat cataaccgtt
    11641 tattatctag gcaattaagg gttttaaaaa atcatatttc tacgctgata aaaactcgaa
    11701 aaaaatgctt ttaaaaaatt tgtattctta aaaaataata tatagcattg tcatgttaat
    11761 ttattgttta accttaaaaa tgaaatgtat gaagatgaca tgccggggtc aattccaaga
    11821 cccgaaggcg gatgcagttg tatataatac actggcatct gcaaacaaat ggaaacattt
    11881 attcagcatt gtatttattg tatttatttg gggaaatgct gattatgttt ttgctcaggg
    11941 tgcagttaaa tactccataa cttatgttac tgagtcgaaa acaacttacc agacaaatga
    12001 ttcattaaac acatttacta tcgaggaaca gttgcaatta atgcctgtaa ctacgcggga
    12061 tagtattatt aaattggtat ataccaataa cgatattaaa actactattt accatcaggt
    12121 gaacactggt tttccggaat gggccacaaa acctgcaaaa actataattg ataaaactcg
    12181 tataaaagta tacgacgatc atggggcatt aatgattaat caggcacatt ctgccaagtt
    12241 taaaagtaaa aacaatcaac ttaaatcttt tttaaccacc aacagttttg acattattcc
    12301 ggattatgtt tatttaagtc ctaccatgaa aactgatttc acagcaaggg gatttgaaat
    12361 gaccaacctt ggtgacggca cttttcgttt tgtaaaagat tctgttacgc tttattataa
    12421 taattcgcga cgaacaaatg aattagtatt aaaagctgaa gatggtacta ctcgacttgg
    12481 aataaaaagg gcgtttcaaa caaatgtaag aggggttatc gtgcctgcct ttgaaattaa
    12541 aacgaaaaag gcggacagtt ttgcaggtca ttgcgttaat gaagtagctt atactaatta
    12601 cgtgtattat gattacacca ctttcgctgg tgggaagtgg gaagatgaag atgaatcttc
    12661 agtggatgat gaacctctgg aattacaagt ttcgccaaac cctgctaata ctatcattac
    12721 tattcaaata cctattgctg ctaatactca aacattaaac ctttatgata atacggggaa
    12781 aatggtttac caatccgtta ttgaagcagg tgagtttgaa atccaaattg atattacgca
    12841 tttggaatcg ggaatttatt atgctcaatt gcaacacagt gaagctttaa ttactacttc
    12901 ttttatcaaa aattaattcc ttaaaaacag caatatgaaa aaaattatct cgctatatat
    12961 tttgctttta attggcattc atttaaatgc gcaattagca ttagactgta ttgatttaaa
    13021 taaatctatt acaattactc ctgctattcc tttcgattat attccgtcat tgtttaccga
    13081 tgatatttgg gcaactccaa cttttattac cgacaggccg gtttatttta ttcacggctt
    13141 aggtggccag ggcgatgaag ccggcactgt cggtattagc tggagccagg catcattgtg
    13201 gtcggaagaa aattattaca tcaattcgcg tcgccccgat tatgccgatg tttctctcga
    13261 ttttgcagca acagaattaa aaggcgattt ggaaaattat ctatccggca gtcctgacgc
    13321 atttttaatt gctcatagtc agggcggaat tgtaagcagg agagtagata aaatgtattc
    13381 cctccacgaa ctgggttatg aaccaagaac ttttggggga ctggttactt ttggtacgcc
    13441 acatcaaggc gccatgattt taaataacag agatgaacta ttagcttgga caggcacaac
    13501 ctgcgaaaaa ttatccgatg gtccggttac ggaattagtt gaagctaatt ttttcctcga
    13561 ccttgcatta agtcctgttg gtattgatac ttttgagaag aaattttgtg atattctcga
    13621 atttaatatc gcaccttttt tatttaaaga ttatttttca ggcattactg agagttatat
    13681 ggtaggctca gaacaactcg ccgaattaaa tgcatttgtt cccgaaattc cttatgtttg
    13741 tttttacgga attgaaactg aacctgtttt atggaatacc ctgacacata tttttccggg
    13801 acatgaacct aataatgtaa taacctacgg tgaagagcct tttggctctg gaggtgatca
    13861 aatgctggta aattatgccg atgctatgat ggataagtat tggacaaaat attatgcata
    13921 cgactacctt gccgatattt attcagattt aatttatggt ttcgatttaa ctaccattat
    13981 ttgttatatg acagcgctat gttatttttc ggcacataat tctctcgaag aatgtgaaga
    14041 tataagtgcg gcatattaca aaggttttac ctggtttacc gatgccaatg aagtatggga
    14101 aggcctaata ggagcaggta gtttacaacc cgaatcggaa acttgttatt gtattgattg
    14161 gggcccgctt ggtgatgtaa atgacgatga atatgaaatt gaacctggaa catcatgtta
    14221 taccgatgac gcaaatacag attgcactac aaccacaaat tacgcatggg ttgttaaacc
    14281 aaatgatggt gttgtgctgg cagaatcggc tagtgaatgt gtggggaatg tgttgtcgtt
    14341 tattggtaag gctatgtatg ggagtaacca ctttagtatg cgaaatgaca ttaatacaca
    14401 atttagatta attgaattat acaatggttt acacggagaa tattttataa atgatgaaag
    14461 ataaaatgca aaaaggttat ttagtgatat tgaaattatt tcttcttgtt tatgcatgta
    14521 aacaagaaga aataatttca aaaaatggag accaaaatgt tgaagatcca tcttcaaaat
    14581 atgtggaatt tttatggaaa tttccattag caatggatac cagcgaaaga gtatcaatga
    14641 cccctgtttt gtctaacggt aatgtagtgt ttactgccaa aatcctttta acacctgacc
    14701 cggcgcgggt aaagtgtatt gataccgcct acagaactat tgtatgggaa tctgacgatg
    14761 tttttatgga agattgtatt gaaatctcaa tggttgatgg ttcaggacac ggggtttact
    14821 taaactattt tgctacatta tgtgatgtca atccaagagt tatcgattta acaaatggag
    14881 atattttgtg ggactataat ggtataggag gtgatggaca tatgtgtctc tggaacgaca
    14941 agttcttcca ttcaaatgta actggtataa atccatttac acatagtgaa attgttatgt
    15001 ctgatatatt aacccagaat ttcagaaccg tttatgctgt ggatatggaa tcaggatata
    15061 gttgtcatat ttatccgccg gctgttatgg ttaacgatga aagtgatact ttgttatttt
    15121 ttcaaaacag acaatggaaa tcgccacctt atgatggtaa aattgatttt tatggataca
    15181 atatgtctga tgatatatta ttgtggaaac ttgacgacat aaatgaatct ggcaacagtg
    15241 ctgtttttat tccgttgata catgataaca aagtgtactt caaagccggt tttgaagtat
    15301 tttgttttga tgctgaaacc ggaaatatta tttgggaatt taatccgcca tcagtctcag
    15361 atatgttact tggtaattta ctaatcgaag aaggtaagct gattgtaaaa acttccggac
    15421 cagaattata tgccatttcc ttagaaacag gtaatataat ctgggaaaat ttaaatgccg
    15481 gtgaaacccc atatagtttg acctattttg aaggcaatat atattatgta gctggtggtg
    15541 acggtaaatt acatgttatt gaggtggcta caggtaagga attctggagt attttgacac
    15601 caaatcaatc tgaatccgta tctttcggca ccactttttt caatggggta gcaattgacc
    15661 cggctacaag gagactttat ataacggaca cacactttat aaattgtttt caactaaaat
    15721 gaccagatat gaaaaattgt attgttttgt ccatcctctt gagcctgctc acaacagccc
    15781 gggctcagga aaacacaggt ccaacccacc attttggtat ttctttaggt gctaattacc
    15841 tctttccttt cgacaacgat ttaaatatcg gtgtgaaaga aaccgggttt aatggaagtg
    15901 ctacctatac cctgcaacaa ggtaaaatcg gtatccaatt atcacccttt gctaaattac
    15961 actcaggtga ggattttgat gcgatgaaac aatattataa agacaaaggt gtaacactca
    16021 atagttataa tagtggctct tatttgtcta caggtatgga tgcaggatta caatttatac
    16081 tcgttgacca tggaaaatta ccggtgatta aaacttatat ccaatatggt atatccagtg
    16141 tgcgcgttcc tgaaattaat tttaccagcg agtctgaaca ggacggtaat gtaaatgtgc
    16201 gtgctgatgg cggcttaggt ttaggttcta acttacaatt tggtgttgtt tgcgatgtat
    16261 taaaatcgaa taataccatt tataatgtaa aactcggctt tatgcaggaa acagcaaatg
    16321 tggcggttcc aacttcaact aatgttgagt cacaatccga aaccattcgt tcggtagaaa
    16381 tgtttgaatg gaggtcgttt tctgcattcc tcaatctttc tgttttcttc gatttataat
    16441 ctattttaca ctcaaaaaaa ttcctacata attttttatc aaaatcaata aatcctcctt
    16501 atcaataaaa tctaaaaggt acggtgtgaa atttttcttt ttgattgcac cagtagtaaa
    16561 atatgaagta gtcgcgatga ttcctatgtt ggcaccttca gttagaatta catttttaaa
    16621 ttacagaata acttcaacac ccactgtatt catggaatcg atgcagattt tatttaatta
    16681 agggcttgtg ttttgggaat tgctaattca gctagttgaa gtaagttggt tttgaaaagt
    16741 tgatttaaaa acagttaatt gattttaata aatggaagtg atgctacagt attgttaaat
    16801 gatagccgaa tggtatatac ccctgcagga agcttgatga ttgaaatgct gttattattt
    16861 gcatcccatt cacctgtaaa taaaatctgt ccaatcgacg aaacaaattc aaactcaccg
    16921 ctgccaagta tatttgaggt gatgttaatg acgtcaaaag aaggatttag agaaattgaa
    16981 caaataatat tttttatatt ttcaattgat aacccttcat ccgccacctg atcacagtca
    17041 tcatctaacc catttaataa ttcagttgca ccagggtaaa tatccggatt ggtatcatca
    17101 caatcggtgt tatctaatac ataaccttct ggtagttcac aggaaatgga atcgattaaa
    17161 atattaccaa aattatcact gtcgatatcg gcgtatgatt gaataaaagc tacattatca
    17221 tccaccaatc catcacaatc atcatctaag taattacata attctgttgc accaggataa
    17281 atagttgcaa gagtgtcatt acaatcaaga ttatttgata catagccaaa aactgtttct
    17341 aagcaagtat caatacctgc atctgggttg ccataagtat caccatctac atcggcaaaa
    17401 aatgtatata aagtaagccc ttcatcaata gctacattgc aattatcgtc tatgccgttg
    17461 catgtttcaa ttataagtgg gtttatttct gcatttaaat cattacaatc aaagctattt
    17521 tcaacataac ctactacaac tgaacaggaa attgaatcag ttccggtagc gccaaacaaa
    17581 tcagcatctg catctaaata ataggtggtg aatgtggcat cttcatcaaa aagaccgttg
    17641 caattatcgt cgatgctgtt gcaaatgtca gttaatgttg gatgaatatc ggggtttaaa
    17701 tcgttgcaat ctgtggagtc gaacacataa cctgcgggca tgttgcagga aatagagtca
    17761 tccgttaaat caccgaatcc atcaccatct gtatctgcaa aatatttgtc ataacaaaca
    17821 tttaatttta ctgtccacat atctgagcca cccccgtttt cagaaacata gccatctgaa
    17881 gagtatgaag tgccagtaat tataaattga ttttcgtcaa gaaataatcc tgcgtatgct
    17941 ctatcattaa gtgaaccacc aagtgatttt tgaaaaacaa gatttaaatt agtatcaacc
    18001 tttacagccc accaatcagc atatccaata gccccaaaat gttcagtaag gtctccgtta
    18061 atagagcctg aggcgcctgt caataaaatc gtagaagaat tgattgtata aatgtcgtca
    18121 aaaatttcaa agttactgcc accaaaacac ttctgagata tgaagtctcc atcttgattt
    18181 aattttaaaa cccacccatc ctgaaatcca tgattcccag aaacatcaaa attatttgaa
    18241 aaagtggtac cgacaagaaa aattgaattt tccataatcg acatggaata acctatgtca
    18301 gtctcagatc cgccaaaagt tttagaccaa attatttcac catccgtatt cacttttaac
    18361 acccataagt ctgtgcttcc tttattttct ggtacatcac cacctgtaga tgctgaataa
    18421 ccaatcaata taagattttc agaatcatct aaaacaattg agtaaccggt atcatcgtaa
    18481 ataccaccat aagaatttgt ccaaatcaac tctccaccaa tattaatttt agcaagtaac
    18541 aaatcgctag tagattcagt cccatggtgg tcatttatat caccattaat agatcttgaa
    18601 tctcctacaa ctaagtagct gccatcatta gactgaataa tatcactcgc cctatcatgg
    18661 gaagttccac caacagctaa ttgccattca agttcaccgg tatcactaat tttaataacc
    18721 caaaagtctg aatttccatg agcacctgta atatctccat cgcttgcgta tgtctcgcca
    18781 gcaacaacat aacctccttc agatgaattt ataattgcat gtgccaatga gtattcatta
    18841 cctccgtaac atttactcca tagtatctct cccgcactgg ttatttttac aattacgaag
    18901 ccagtatatt caccacaatc aaaaaaatct gctgctaatg gtcctcctct gcctactaca
    18961 aggaaatttg aatcggctgt tgcaataatg tcaaatgctt cttcataaat tatccctcca
    19021 aaactctttt cccactcaac ttctaaagct tgtgcttgtg ctattattgt tgagataatt
    19081 tgcattaata ttaaatgtaa aaacttcatt ttcaatcagt tgggatttaa ggggttaaaa
    19141 tttcaagcaa aatacaaatt tttcacaaga gttcaaataa attttctacc ttcaatgaat
    19201 taattaacca tttaaataca taactatgaa aatcaaacat gcaatgtatg tattgttaat
    19261 actttcagca atactaagta tgcaaacttt ggcttacccc acagaaccta ttaaattctc
    19321 ctacgagaat tacttaacaa gtcttataga gtctgaaaat ttcgccttac cagatacatc
    19381 ggttcagatt gaagaaggag ataccatcgc aaaatacacg gctggaggtg atagtgtcta
    19441 tgttctttat gaagactcaa tccctgactc atcctttaat atggcttggg aaattccatt
    19501 tccgacagag catatgatta ttgaactcac taatcatcat ttacctgttg aggaaggatt
    19561 gtttgggata aatgtaacag atatgttcca aacaaaattt gcgaatgagg atgaactacc
    19621 tacaatttat ggtaccaatc ctgacccatg ggattactta agtcaattgc agccaaaaat
    19681 attaaggttc ccttccggtg caggtggtaa attttatcaa ccactaggtt caatacgtac
    19741 caatcctgat gaccctgatg ttggatttat aaatggtggc tatggaataa atattgatga
    19801 agttattaag ttttacgatg ctaccgcaac tggggtaggt gacgcccctt ttttattcga
    19861 tccaccaggt gatcctggtt acatttcaat aacagatgat atgggtgata ataattgtga
    19921 taattgcatt ctttgaatgg atgatagcca cacttcaacc tttgaagata actacactaa
    19981 gtgggacgat caaccaatca ttgatccgac aggtattgtt gagccttcag acatgggtcc
    20041 tgacctttat gtcaatcaat ttataagact ggtagaaaag attgaattag aaaactccct
    20101 tttacttgga tacacttatg ttgttgatgt agtagtttgt ttaaacattt caactcaaac
    20161 agctacagag gccgtggagc taatagatta cttattagaa tcaggaatta atgtggttgc
    20221 tgttgaaata ggtaacgaag tgtactttga ttggggtaga caaatgatgg gatttacgga
    20281 tcacttaaat cctccattag gaccaacaag tgatgttagt ccttttatcc attattggga
    20341 ttatataaat ggttttgaat atacggacgg agattctggt gttgatcccc tggatggtga
    20401 atacggagat gaagatgttg atttagatct tgcctcagta ctacctactg acgtattact
    20461 tgaccatgat tatattactg caattaacac agcatttcca gatattaaga ttggcttacc
    20521 cggtgaaaat tttaatcagt gcgaggaagt gggatgggat acaccgttta ttgtagacga
    20581 ccccgatgat gaaagactat ttttacctgg tggacctgaa ccagagtgca attgcgatta
    20641 ctctgattgg aatatagata tggaaacaaa gtttgatgag gaaataggag ccggagaaag
    20701 tgcaggccca aaatttgatg caataatatt acatacttat tataatgccg gaaatgcata
    20761 tgttgactgt cctgacaata ctaactggca agttgcggca acagaaccaa cattcgttgg
    20821 aggaacacca tttgaggacc aatggacaaa ttcacctttt gacccaaata tcgggacatt
    20881 atttgaaggt attgctggca ttgccactaa ccctgatggt gttggagtac ctgagtacca
    20941 aattggaaac tttaaagaat tcacccgtga tagaataaaa tatgcttacg accaacatgc
    21001 aataaattta aacttcctag atggagcccc tggcagtact aaagaaattt ggattactga
    21061 atataatatt tctccaaaga cgagtggttt aacagcggag gatgatgaat cttttactaa
    21121 tatggtacca aacacctttg cgcactgtgt cgcattacaa aattggatgc tctggcacgt
    21181 taagtcctat tatgattccg actacaaacc tgggattgtt acaatggcaa caacgcaagc
    21241 ctttatcggt ggaaatgcta tagattatat gacaccatct aatcgcaatg accaagtaga
    21301 attagagact ccaaacccag aattgacttg cacatcaatc gaatactcag atgttgagaa
    21361 ttactttgta agaagagctt catattttac agcacaacta cttaataaca ttcacactct
    21421 agaccttaac tatgaaaaaa catttatagg aatgtatagc caaaacaaca atattgcacc
    21481 aacaacattt gtgcagctag acaatgatgg tgaaccaata aatgtgtatg tcttttatac
    21541 aaacattaag caaactacgc agcgttatgc tattgacccg gctgatattt atttatccat
    21601 tgaagatgct gggggtgtgg aattaggcgc agcaacagta tatgccttga atcctagtca
    21661 actatattcc acctctggtc gcaatcaaat gttttatgaa cccatcaacg aagcttatgg
    21721 ctgtgcccct gtattaccag attcacccta tgtttttgag ataacagata cgtggacagg
    21781 tttaagcggt attggctgcc caggtggttt tacgccacca ccaggcggag tttgcgttac
    21841 agtgcctgct acgtctgctg gttattttgt aattccgatc atgccatacg aaaggaaggg
    21901 agaaatcgct tcaacattta gattgtatcc caatccagct gatgattttt ttgtaattga
    21961 aaaaacttca acaaatgaaa atgttagcga tgattttaca atgagaatct attcaacctt
    22021 tggaacttta gtgatagaat cgtcaatacg ctcaggtgaa atggttaaca cagatatttt
    22081 atcttctggc atgtatacag ttgttattat tgattcaaac aacaatgctt ctactttgaa
    22141 attttctaaa atgcaataaa ataaaccaac tgttacttaa gtgggggctg tctcaaaaga
    22201 tgaggcagcc tcttttttat tcatgagctg tctgagtaat attagtttaa gcaattctaa
    22261 acatatgctt ttattgattt ttgagaccgt ctttttttga ctggaatagg atattaaaaa
    22321 acttcatcta caatagtgcg catcgcatca acattgccca tggtataata atggatacat
    22381 ggtgcacctg cagcaactaa ttcctgcgat tgctgaatac accattttac acctgctgct
    22441 tttaccgctt caggcgtttt gcaattatct atttcatcgc tcaaagcttc aggaatgtcg
    22501 acatggaatg tacgtggaat actatgaatt tgtgatgctg cggtgatagg ttttataccc
    22561 ggaataatag gaatgttaat accttcttca cgacactttt ttacaaaatc aaaatatttc
    22621 tgattgtcgt aaaacatttg tgttactata taatcagcac ctgattcaac cttttgtttt
    22681 aagaaacgta aatcactttt taaattcgga ctctcaaaat gtttttcagg ataaccggca
    22741 acaccaatat taaaatcggt tttgtaaccc tgaatactgt cttcctctaa atagatacca
    22801 ttattcatgt tcaccacctg tttcaccaat tcacttgcat atttatgccc accgggttca
    22861 gcaataaaat cacgctcatt tttcgaagca tcaccgcgta ataataaaac attatcaata
    22921 cttaaataac ttaaatcgat taatgcatct tctgtttctt ctttggtaaa accaccacaa
    22981 attatatgtg ctaccgcatc aattttatat ttaaattgga tagcggcaca tataccaacc
    23041 gttcccgggc gctttcgaat aaataccttg tcgaatctgt catcgccacg tttcttataa
    23101 atgtattcgc ttctgtgata ggtaacatta ataaacgaag gattaaattc gattaagtga
    23161 tctaatacat cataaatccc ctttatactt ttgcctttta atggaggtaa aatttctaac
    23221 gacaacaagg ttttttcggc cttggctaaa tgttcggtaa ctttcattga tactaactgc
    23281 ctgtttaatc catcagcaac actgctaata aaccataaag ataggaacgc atgattttat
    23341 attcaagcac tgtaatacac ttcgctgatt attgcacaat aacgcttcct gctcccacaa
    23401 acaaatgcct atctttgacc catgaatatc ctacttttag gttccggcgg ccgtgaacat
    23461 gctttggcat ggaaaatagc ccaatcacct gattgcacta aattatatat cgctccgggt
    23521 aaccccggaa ctgctgagct tggaacgaat gtcccaataa aacctaccga ctttgatgcg
    23581 ttaaaatcgt tttgtttaaa caatcaaatc caaatggttg tggttggtcc cgaagaccct
    23641 ctggtgcacg gtgtagtaga cttttttgaa caagatgctg ctgttaaaca tattccggtt
    23701 attgggccta ataaaattgc tgcacaaatg gaaggaagta aaacctttgc caaagctttt
    23761 atgttgcgca ataacgtaca aactcctgcc tatcgtgaat ttaatcaatc aaataccgaa
    23821 gaagcaattc agtatgtcaa acaacacagt atgccggtgg tattaaaggc agacggactt
    23881 gcagccggta aaggtgtagt aataagtaat acacctgatg aagcagcaaa cgaactacaa
    23941 cttatgatgg gtggaaaatt tggtgatgcc ggtaataagg ttgttatcga acaattttta
    24001 catggtatag aacttaccgt aattgtttta accgatggta attcttatat gattttgcct
    24061 ccatctaaag attataaaag aattggagat ggtgatacag gattaaatac cggtggcatg
    24121 ggtgccgttt ctccgcctcc atttgcgaca aatgatttta tgcaaaaagt aaaatcaaaa
    24181 attattgagc caacacttga tggtttaaaa aaagaaaata ttcattacaa aggtttttta
    24241 tatttcggat tatttaattg caataatgaa ccttatgtga ttgaatataa tgcgcgtatg
    24301 ggtgatcccg aaacacaggt cataatgccg cgtattaaaa ataatattgt tgaattattt
    24361 actgctgtag ctaatggcac tttacatacc aaaacattaa ttacggatga acgtgcttgc
    24421 acaactgtaa ttcttgccag ccatggttat cctgaaacat ttgaaaaagg attccccatt
    24481 accggattag aaaataccga aaactgtatg gtatttcagg caggaaccgg tttgggtaat
    24541 aatggcgaat tggttacatc cggtggcaga gtaatggccg tatcggcata tggaaacgat
    24601 ttggcagaag cactgcgcaa ttgttatatc aatgcaggaa gaatttatta tgattcccgt
    24661 tacttcagaa gagatattgg attcgacgtc atgtaaattt aattcctact attttatttg
    24721 ttgcgattta aattgaggtc gactagttgc tcaattgcta cttgttgtca atctagtaaa
    24781 atacatattt gaaacgaatt gtgtttggct aatcttaatt aaggattatt gttacctgta
    24841 gttttattag gtgtgaccaa atactttatc cttgtttgta aaatccgggg ttatactttc
    24901 tccgtatatc tctccaacaa acaaacaaca atgaaaactt ttttattcgc agcaatcaca
    24961 gcaatacttt ttgcaagttg ttcaaaacaa acaaactgta ttggcagcga ttgcgtgttg
    25021 cctgaagcag ctatcgccat cgactcagta acaaacaatg gtattttcgt tcgtttaccc
    25081 aataatgaag gtggcttttt tgcctgcact ttggaactgg cttcccatat caatcaggat
    25141 gaatttttag cggctgtttc ggcttatacc actgcatcac cggttccggg tagcgaaact
    25201 caaacaatgc ccatgcttaa agtatattat gttgcagagg accaatccta tgtgcgcgaa
    25261 aatgcacgat atattcatgc ctcaaataat aaactggaaa ttatcagtta cggaaataac
    25321 cttgaaaccg actgatttaa taatgataag tgttttgtgt taatacaatt tttagcttgg
    25381 ttgaattaga aaaaaccgca ttcttcatgc ggttttttgt attttatatg tatttacata
    25441 cattttttcc cgaatctatt aagtttctgt tgcctcctaa aaaccttatc cggtaagggt
    25501 tttggcggca tctctttcac ttaataatac ccctctcttc tcggaaatgc catttttctg
    25561 tcccgttttc cacgcaactt ttcccggttc actgccgttt tacctttcaa ac
//
LOCUS       DAORKW010000005        24550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_8_length_24550_cov_5.981384, whole genome shotgun sequence.
ACCESSION   DAORKW010000005 DAORKW010000000
VERSION     DAORKW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 24550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24550
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8_length_24550_cov_5.981384"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLB46_00520"
     CDS             <1..732
                     /locus_tag="PLB46_00520"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRF75021.1"
                     /translation="ATGAQLYITLLFFAVQLYADFSGYCDIAIGTARLFGIKLSENFN
                     QPYIATSISDYWNRWHITLTTWIRDYIFTPLNKGVTTYWRIYLNTLIVFILIGLWHGA
                     SINFVVFGLINGIIAVLQAIYKRISFLPKFKSNYGKIFLIIWNFHLLILSGVVFRAKS
                     FTDARLYYQKLFSGFSVELQQILNGFAAYEFAICLAVALIFVASFWLPKNFRFKYSYA
                     FILIVTGMVILLGRNNAETFIYFQF"
     gene            complement(733..987)
                     /locus_tag="PLB46_00525"
     CDS             complement(733..987)
                     /locus_tag="PLB46_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75022.1"
                     /translation="MDQQLPKHDCRNFVKKVFLVLDGELPETERNLFIMDIQRCKGCL
                     EHFHIEKTFKDFIAAKVERKDCSEKLKQTIISQVQSNPET"
     gene            complement(990..1568)
                     /locus_tag="PLB46_00530"
     CDS             complement(990..1568)
                     /locus_tag="PLB46_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF75023.1"
                     /translation="MEKGNLSKSSKDRIFNKEFLPQMDALYNFAFHLTLDEDDANDLV
                     QETFLKAYRFIESYQQGTNAKAWLFKILKNGFINEYRKKTKQPSKVDYDEVASFHDTD
                     DEGHVEYLDLRHELFQGMLGDEVTRALNQLPVDFKTIILLCDIEEFSYEEIAKIIDIP
                     IGTVRSRLHRARNILKEKLREYAQKEGFKEKR"
     gene            1721..2983
                     /locus_tag="PLB46_00535"
     CDS             1721..2983
                     /locus_tag="PLB46_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRF75024.1"
                     /translation="MRSLLRVFPNQFFASLAILGLIVGLVTSKVVLSVATIMIMCNAI
                     INLRIAENFKKWLGDTTSVLLIALFLVYLFTGFYSDDTGYWVDRCRMKLPFLALPLGF
                     TTVKTLTHKRFQQWLAVYFYVIVAAAIVVCINYVLHFDTLNEQLTKGQPIPAPLRDHI
                     RFSLEMAFAIVVGIYLWNNKFSFGLRNEKYILGGGILFLTIFIHVFAVRSGILTMYIA
                     FIVMILYWLFTKKAYLQGLVALIALVGFAFSAVMYIPSLHARMLYFKYEMDLIKKGEL
                     HPEHSDAQRLLSIQYGVQIAEENPLLGVGVGDIKTEMDKLYNQSAGSEFVKSKLPHNQ
                     FVYFLAASGIIGFLVFMLSVLYPWFSHKRFKNMLYSVFILIMLISFMAEHTLEIQIGT
                     AFYLLFLLLIKKYIDDTAQLAESEKQYA"
     gene            2976..3734
                     /locus_tag="PLB46_00540"
     CDS             2976..3734
                     /locus_tag="PLB46_00540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF75025.1"
                     /translation="MHKVTAIIPCKNEAHNIEAVLESVKWCDEILVVDSFSTDNTIEL
                     AKKYTSRIIESEYINSASQKNRAIPQAAHEWILLVDADERVTPELTNEIQQLLQSDVI
                     PHDAYWIYRKNYFMGKEVKYSGWQRDKVIRFFKRDTCRYEEKHVHAEVITSGSVSKLK
                     YKLVHNTYKDIFHYLEKWDRYSTWSANDAAKKVTRPGFYHFIIKPGFRFFKHYIIDLG
                     FLDGWTGFIVCSLAAKGVFMRYVKLYGMIKSNKN"
     gene            complement(3735..6095)
                     /locus_tag="PLB46_00545"
     CDS             complement(3735..6095)
                     /locus_tag="PLB46_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75026.1"
                     /translation="MNTLHLQENSIAFVDAGYVEGNIIRLKKNVPLTSVIGFHFWVEK
                     AGVVEFNFPVGAEKCKVHVYKLPIQTPYPVLVNGNYFKEPISVAYGNYLMVIENFQEV
                     DIFRDAVIFKPSSTAIVAQPILKPNKIAFSPIPGNIFQTLKKIGITDLKQLNKKNHIK
                     LLRSELQNIELENEILQLNKYLQHPGINYGNAVTMFRKANKLNGQPIINNVNNIIAAD
                     GEIVVDPLDETLPCVQVGTISGIPDPLATEPIVDWFWYDAAGDFDHEIITRPWWASPD
                     NFINGPNEVQDYWYPDPNKGWRVFGYQFGGSPGQIGYLLCYNVFTGVMRLFVYLPNVE
                     SRNFNKLIATFSLVNADRIASQTWNFPLMDVPPEIKSFQTSDTPTADNVQEVTGYPGG
                     SAFIWPGLEMAFHDLTASSASAPGKWLRTEIATLFDPALYPETQILRGLGGCLGLLFP
                     GVKAPTSELQERDRRLFKIDFNGILEGQSNLTADLELTASGTAKPVAEESSTSSLGID
                     AEYVAYAKKAIQGASIIGGFISTAVATGGLDIMSAVKAAGGIYALFQGDENQNPSYDV
                     QLIGAITGKITGRTVFVLPVADFVVNLNGTFKAVTEMTNNGPVYFPTGWPHTYQRCNK
                     LRIGVIGFRPAGEQGQVLGTDPRPATIKLEWIARDELGVVFDALVTISPIGLFQIAPW
                     ANVIIDEQYAELELFEPQNDLPHSVTTIRRLNVNALNVYRVPSVPYLLGLNEHKRMRI
                     RWVAKMSTPNNELPPFIIEYALDLTGKTNFILDTKPSSGDFPDDDW"
     gene            complement(6264..7535)
                     /locus_tag="PLB46_00550"
     CDS             complement(6264..7535)
                     /locus_tag="PLB46_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HRF75027.1"
                     /translation="MMKTFFTVFFLIVSLFLSAQDYCTMSRFDEFVFDDNEIEILANV
                     KYGEAVNNLGNNQNLFLDIYKPKTAFDELSKRPLIFFVHGGGLVGGSKDSQGAVDLGN
                     LYAKMGYVYVSIDYRIGWDNGDADDGCGGDTTDLFRATYRAVQDVRAAYRFIKGEADV
                     YGIDTNVIFVEGNSAGSRLILFALYASQSDYNTQFYDELGSIDTAVNDYENYHFNPIG
                     VITEAGGIEDTSLLYNNNYPHLYFHGTCDSIVPFFSGPTFFCYQPFEYPNLHGSWQLT
                     QLSKRLGRTVQFYIGEGAGHDAVIPDSIILYAAPFMKDIMCNTLTSKDIYRVLGKYKC
                     AVANEGELVIVDIYPNPVVDNIYLQVTSTRDKEIEMAIYNSIGQVVLSYNLEFYPPIK
                     DYTINVGDLARGIYYLRISQRQEIYVAKFLK"
     gene            complement(7570..9168)
                     /gene="nadB"
                     /locus_tag="PLB46_00555"
     CDS             complement(7570..9168)
                     /gene="nadB"
                     /locus_tag="PLB46_00555"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRF75028.1"
                     /translation="MHKADFLVIGSGIAGLTFALKTARRFPDKTITIITKAEEEETNT
                     KYAQGGIAAVWDFEKDSYDKHIDDTLIAGDGLCKRDIVEIVVKEGPERVKEIINWGAD
                     FDKNKEGEYSLGKEGGHSEFRVLHHKDVTGKEIEKTLLEEALSLPNISIHAHHFVIDI
                     ITQHHLGRIVTRHTPDIECYGVYVLNKKNKTIEKIIAKITVMASGGAGQVYKNTTNPL
                     IATGDGMAMVYRAKGRISNMEFVQFHPTSLYQPGVSPSFLITEAIRGAGGELKTSDGK
                     PFMHKYDERKSLAPRDIVARAIDNEMKIRGDEFVYLDCAPIPKEELLNHFPNIYEKCK
                     SIGLDILEDFIPVVPAAHYMCGGILTDAHGNSSINNLYACGECSSTGLHGANRLASNS
                     LLEALVFANRIYEDAAAKIDNIEFQENIPDWNAEGTNEPKEMVMITQSIRELKEIMTN
                     YVGIVRSNERLERALNRLHLLYEETERLYDTTAISPQLCELRNLITIGFLITRGASAR
                     KESRGLHYTTDFPERSDWAEDSLF"
     gene            complement(9289..9861)
                     /locus_tag="PLB46_00560"
     CDS             complement(9289..9861)
                     /locus_tag="PLB46_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HRF75029.1"
                     /translation="MIVIQQTLVSEDILDKEFVCNLNACMGACCVEGDEGAFVDEDEK
                     KMLKKVYPVVKEYLTKEGIDAIEKQGYWVTNDEGVLKTPLMKDGPCAYTIWEKGIALC
                     GIEKAFLDKKVKWQKPVSCHLYPIRISKVGEFDALNYEKWNICKPACKNGKELGVPIF
                     RFVKTALIRKYGEEYYEALEATYAYKQTNK"
     gene            complement(9917..10615)
                     /locus_tag="PLB46_00565"
     CDS             complement(9917..10615)
                     /locus_tag="PLB46_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRF75030.1"
                     /translation="MHGTLFLVPTILSEEALHVIPQYVFDITNKLTVFFVENEKTARR
                     YLRKSGFTASFETTPILPLNEHTDAATVSTYMEYLKQGTDCALMSEAGVPAVADPGSV
                     LVRFCHQNNIRVVPLVGPSSILLALMGSGLNGQQFHFHGYIPVKQPQRKQYIQHMEAE
                     TKKGVTQIFIETPYRNNAILQDILSACSSDVLLCIAYDLTGTKESIRTQPISYWKKHM
                     PALEKIPAIFLLGM"
     gene            10690..11295
                     /locus_tag="PLB46_00570"
     CDS             10690..11295
                     /locus_tag="PLB46_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRF75031.1"
                     /translation="MNNFTLKKSFYTHSDTLGIARALLGKKLMTSFEGKVTAGIICET
                     EAYCGVEDRGCHAYNGRFTERTKIMYAEGGVSYVYLCYGVHYLFNVVTHVAGQPHAVL
                     VRAIEPTEGIPTMLERRNLKQFTPGIGAGPGLVTQCLGLTTNHNGLSLLGQDIRICDA
                     APINPKQIVASPRVGMNFSGPYKLIPWRFRIANSPFTSKAK"
     gene            11297..12133
                     /locus_tag="PLB46_00575"
     CDS             11297..12133
                     /locus_tag="PLB46_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRF75032.1"
                     /translation="MRKKLNTVIVVLRTTEWKLVFKPFGYHKLLLALFGFFLTGSTSG
                     LIAGLIIGIIMDCEIIFKEKPSKKSTDNRISYLMLAAFVLQAGELNGRISNETLHRRL
                     VNKFGEVYATKRFAFFHELFRQRIQVEAICEQIKLHADLKDKKDIILFLFEISYHPSA
                     PKEKVTHTINYLAARINLPYEDVKTIYRTFSQQHQNNYSSNTKTYTEKTTPKSDIYTL
                     FNLTSNCTEKELKTAFHHLAKKYHPDSNPNASNAEKITMQEKLRTIIESYDEIKVRRG
                     WK"
     gene            complement(13002..13871)
                     /locus_tag="PLB46_00580"
     CDS             complement(13002..13871)
                     /locus_tag="PLB46_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF75033.1"
                     /translation="MRQLKITKSITNRESESLEKYLQEIGKVDLVTAEEEVTLAQKIK
                     QGDQLALEKLVKANLRFVVSVAKQYQHNGLSLNDLINEGNVGLVKAAQKFDETKGFKF
                     ISYAVWWIRQSIMQALAEQSRLVRLPLNKVGSLSKINRAFSELEQKFEREPTSEELAE
                     VLELAPEEIKNTLSVSSRHVSVDAPFDDGENSSLLDVLENRDTERTDEKLDYSHSLKV
                     ETERTLATLTERERDVIKLFFGIGVPYAMTLEDIGEEFGLTRERVRQIKDNAINKLRS
                     STRNKALKAYLAQ"
     gene            14152..17226
                     /locus_tag="PLB46_00585"
     CDS             14152..17226
                     /locus_tag="PLB46_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRF75034.1"
                     /translation="MRKWLLIYALFSGFSAYSQAFYEGPSWTDFGDLTYTAFGWSMSN
                     AGDVNGDGFEDMIVSAIDYSNPEETNGEEGKLALYYGGPDGLETEPAWEFESNNDSSV
                     LGFSTSGGDLNGDGFSDVVVGCLQWTGEEYNEGRIYLWYGSESGLNAGEPDWSLDFDQ
                     VFALLGSGVALDGDINGDGYNDLFLSAKMWDEPEIEEGKTWLYWGSPDGPVESGWSWQ
                     ANQEGAISGFPVNYAGDINADGFDDVIIGANQYDFDEIDDGLAVVFYGSETGLAAEPD
                     WMMSSGQKKCNFGHWVDGAGDVNGDGYDDVVVSALLYESDSASHNEGRIFVYHGSATG
                     LSNELNWFGEINQAEAQFGYSCAGAGDINNDGFDDVIGGAKYWTNGEANEGGAFVWFG
                     SPTGLEIDYCFSAEGNQAEGYFGRHVGGDADYNNDGYSDFFVGAYRYSEILAADGKAF
                     AYNGATRPADFYFEEDTFCIDDVNPLPIITGFTGGTFSSADVVVEAISGEIHLMESGF
                     GGPFIITYTVDGYCSVNKTIWIQDANALTLFNYENDSLCLNDAIIIPEIIVPSTGTFY
                     SDDIIVDVINGAINTTDVAAGWATIYYAGSTAAGCNFIDSIALYFQEQVTINLDQDTF
                     CIDLGEAIPLVNIPGGNYFSPDAIVDVLTGTIDLPSTGVGGPYQIYYNVNAICPDASF
                     IFYIDSADNNLVAFHFEDDTFCLYDLNPSPVITGLAGGIFSSATAILNDITGEVNIIA
                     TGAGTHVILYQLTSPTGCSLMFTDSIAINNPPIVAITYPIDTLFIGDENVTPEILGLT
                     GVINAIPDGVVFADDFGTIDLINSTPGNYSIYITVEENGCYNSDSATITILPACEAPQ
                     NIVVDYYNSNSVIISWDEHDFYTDYTILVILGDDTLTYYATENTITITDLLPDTTYTL
                     VVITNCSSSHNNAGNPLVITTAALAIEDNLGFNMLIFPNPTSSDFTISTPLLLNGCKL
                     SIFNVEGKQVYLETQLPKQNTFLITSHQLISGLYFIHLTNGNQTTFSTIVIE"
     gene            complement(17263..17586)
                     /locus_tag="PLB46_00590"
     CDS             complement(17263..17586)
                     /locus_tag="PLB46_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRF75035.1"
                     /translation="MKKIILMLSLFIITGSLSAQSKQYIEIKTSAQCATCKAAIEKAV
                     GAIEGVKLADLDLDTKVIGVKYDSEKTNAAAIKTAITAIGYDADEVPADAKAYEELHS
                     CCKKE"
     gene            complement(17604..19820)
                     /locus_tag="PLB46_00595"
     CDS             complement(17604..19820)
                     /locus_tag="PLB46_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF75036.1"
                     /translation="MKYYNYILLVLLGLSKISFSQDVSGHVMSTDEQGKMVGLPGANI
                     VWLNTTTGTVSNELGFFTLNRTDNNQQFIVVSYVGYENDTLDTKNNNSLHVMLTPIAD
                     LAVVTIEGETGGRTINTINTINTETLNRKELLKAACCNLSESFETNNTVDAEFSDAIT
                     GAKTIKLLGLDGVYSQIMVENMPNVRGLASSYGLTFIPGPWIESIQITKGPGSVVNGY
                     EGITGAINTEIKKPYDIDDEIWLINLFASNSGRYEANVNFKHLFNEKWATALYTHTSQ
                     LQNKLDHNGDTFLDAPLNSTYAVMNKWNYFSDNIHEAQFGFKYVKSDLTGGQINYDKS
                     LEPSVNNGYGVEVNINRFEAFMKNGFVFDRPNTSIGTIVNFAQHEQNGLFGLNRYNGK
                     EVYASANFIFQSFILNTNHTIKTGASYLYNDFDEVFDSINYLRKESVPGIFAEYQYKP
                     DDKLSILVGVRGDQHNLYGTFITPRLHVKYNAFKSTTIRASIGKGYRTANVFAENTTL
                     LTSNRSLVITESLLPEQGWNYGFTVIQPFKLNFREGTFTLDVYRTDFVNQVVVDIDSD
                     DDSIYIYNLKGDSYSNVLQAEINYELLKRFEIRTAYKYIDAQETFNGELLEVPLTYKH
                     RGLINLSYEWKRAGLLFDATSQFYGTSRLPNLSDNHEAHDLGTTSPAYTLFMGQITKQ
                     FKKLEIYAGSENIGNYTQHEPIIGATNPFGEDFDASVIYAPLMERKFYGGIRLIFN"
     gene            complement(19894..20292)
                     /locus_tag="PLB46_00600"
     CDS             complement(19894..20292)
                     /locus_tag="PLB46_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75037.1"
                     /translation="MNKVFKRFGAYLMMVVLFTTSTGFTMFSHMCFMDGQQAVSASKI
                     DSCCPIDQVSDITSLTSDCCDDETNFYKLDYQASVQRSDDNSFVLMATFLFQSFTASE
                     TSIVALTNYSPQPPPKSGREILSDIRTLQI"
     gene            20422..21237
                     /locus_tag="PLB46_00605"
     CDS             20422..21237
                     /locus_tag="PLB46_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75038.1"
                     /translation="MKMKKSVFLLTLTTISAVMFLSSCAKKEGCMDQAALNYDPEAEV
                     DKGCEYASDDDVAVTLHMHQNIGGGELVEETAYTINGVVTNLNLVQFYVSAITLVDAA
                     GNEITNDDVYLLVKPEEEAYAIGNFPAGDYTQIRFDIGIDSVTNHADPSKYDIGNPLG
                     AQFPAMHWGWSFGYIFVRIDGEADADGNGVPDPDGQFEMHLGSDAYVATIEIDLPITI
                     AEGNENIVHLDANWDTFFTGVDMANDNTTHVTDNAALSSLLYENIFNMFSAEE"
     gene            21238..22275
                     /locus_tag="PLB46_00610"
     CDS             21238..22275
                     /locus_tag="PLB46_00610"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRF75039.1"
                     /translation="MKKQNTTYYICLFFAGIIFSSCAKDPKIIVEPDAYVLNVPSGFV
                     FPNIPEDNPLTTAKIALGKKLFYEKALSVDSTISCGTCHKQEFAFSDNVAVTAGVEGR
                     LGFRNAPTLTNIAWSPEMLMDGGNPTLETQVYVPIETHFEMDFNMVLLVNRLNADDEY
                     VDAFNNVFGTNPDPFGITRALAAFERTIISGNTRFDQYYYQGKTEVLNEQEINGMQLF
                     FSPTLKCASCHSGYLFTDFTYQNNGFFADYSADSGRARISLLSEDVGKFKVPTLRNIA
                     LTGPYMHNGQINSLEAIIDSYANGGSGHVNQSELIQGFVLTAQEKENLILFLNTLTDN
                     QFINNPDLGED"
     gene            22321..23427
                     /locus_tag="PLB46_00615"
     CDS             22321..23427
                     /locus_tag="PLB46_00615"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRF75040.1"
                     /translation="MKWRSLTILFFSFFVILFTQCKIDPEIISGEEWVYDPTPYAVMS
                     PMGIPNVPNTNGNPLTAKGVELGRYLFYDPILSADSSLSCAGCHSVNHAFSDTVKFSI
                     GIDGIAGKRNAMPIFNLGFQPYDNGFFWDGRATTLEDQALMPIQDPVEMHEILPNVIT
                     KLSRSDIYPKMFYEAFGESKITADLTGKALAQFVKSLLAGNSKFDSVVAGFAFFTDDE
                     LAGFLSFESLEGGDCFHCHGVNGGLFSDYIYRNNGIDLANRYFDFADAGYGLITGDTS
                     DYGKFKTPSLRNIELTAPYMHDGRFATLEEVLDYYSEGVNDTQFTDNFMQFAFQGGVQ
                     LDATEKAQLIAFLKTLTDHTLKDNPIYQNPFEED"
     gene            complement(23430..23900)
                     /locus_tag="PLB46_00620"
     CDS             complement(23430..23900)
                     /locus_tag="PLB46_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75041.1"
                     /translation="MSLKNSPLLLLAAIIISSCSGKKSTPENVALEFYNQLFNEHNLE
                     KASELVTAASKEKLSNDFKFIEGALQIVQEATPTTYIYKVDADKSKIGNDSAFIHIWT
                     SLDSTTMETLLIKENETWLVDFNYTPPVDAFNKELVDDVLKEMQQFVDTVTIAK"
BASE COUNT         7523 a         4552 c         4416 g         8059 t
ORIGIN      
        1 gccaccggtg ctcagttata tattacgcta ttgttttttg ccgttcaatt atatgcagat
       61 tttagtggct attgcgatat tgccatcggt actgcccggt tatttggtat aaaactcagc
      121 gaaaatttta atcagccgta tatagcaact agtatatccg actactggaa ccgttggcat
      181 ataacactta caacctggat tagggattat atttttacgc cacttaacaa aggtgttacc
      241 acttattggc gaatttatct caatacttta atagtattta ttttgatagg attatggcat
      301 ggcgcgagca ttaactttgt tgtttttggt ttaattaacg gcattattgc tgtattacaa
      361 gccatttata aacgaatatc ttttttacca aaatttaaat ccaattacgg aaaaatattt
      421 ttgattatct ggaatttcca cttgttaatt ttaagtggtg tagttttcag agctaaaagt
      481 tttaccgatg cgcgattata ttatcaaaaa ttattttcgg gatttagtgt agaactgcag
      541 cagatattga atggatttgc ggcttacgaa tttgctattt gtttggccgt agccttaatt
      601 tttgtagcct ctttttggtt gcccaaaaac tttcggttta aatacagtta tgcgtttatt
      661 ttaatcgtaa ccggaatggt tattttatta ggaaggaata atgcagaaac gtttatttac
      721 tttcaatttt aattaggttt cagggttact ttgcacctga ctaataattg tttgtttcaa
      781 tttttcactg caatctttgc gctccacttt ggcagcaata aaatctttaa atgttttttc
      841 aatatgaaaa tgttcgaggc atcctttgca gcgttggatg tccataataa ataaattgcg
      901 ttctgtttca ggcaattcgc catctaaaac gaggaaaact ttttttacaa agtttctgca
      961 atcatgtttg ggtagctgtt gatccataat tatctttttt ctttaaaccc ttctttttga
     1021 gcgtattcgc gtaatttttc ctttaaaata ttgcgcgccc tgtgaaggcg tgatctaact
     1081 gttccgatgg ggatatcaat aattttcgca atctcctcat atgaaaactc ttcaatatcg
     1141 cacaacagaa taattgtttt aaagtccacc ggcaactgat tcagcgcccg tgtaacctca
     1201 tctcccagca tgccctgaaa taattcatgt ctcaaatcaa ggtactcaac atggccctca
     1261 tcgtccgtat cgtggaaact tgccacctca tcgtagtcaa cctttgatgg ctgcttcgtt
     1321 tttttacggt actcgttgat aaacccgttt ttgaggattt taaacaacca agcctttgca
     1381 ttagtacctt gctgatacga ttctataaag cgataagctt tcaaaaaagt ttcctgtaca
     1441 aggtcatttg catcatcttc gtctaaagtt agatgaaacg caaagttgta cagggcatcc
     1501 atttgaggta aaaactcctt attgaatatc ctgtctttgc tgcttttgct aaggttgcct
     1561 ttctccacga gggtaaaatt agaacaaagt tgccgaggtt tcaaatcagc aaaaattata
     1621 gtattgtagc ttttaatatt atttttttac ttttggttca ctattcactc aaaatctaaa
     1681 tagtccctat agaccctacc tctacgaatc taccttcgac atgcgatcat tacttagggt
     1741 ttttccaaat cagttttttg cctctttggc tattttaggg ttaattgtag gcctggttac
     1801 gtctaaagtg gtgctcagcg ttgctactat tatgattatg tgtaatgcca ttattaacct
     1861 gcgcattgcc gaaaatttta aaaaatggtt aggcgatacc actagtgtat tattgattgc
     1921 cttatttctg gtgtacctat ttaccggatt ttattctgat gatacgggct attgggtgga
     1981 cagatgtaga atgaaattgc cgtttttagc tttgccgctt ggatttacta ccgttaaaac
     2041 gctcacacac aaacgtttcc aacaatggct ggcagtttat ttttacgtta ttgttgccgc
     2101 agcaattgta gtttgtatta attatgtttt gcatttcgac acattaaatg aacaactcac
     2161 taaaggtcag cctattcccg caccactccg cgaccacata cgctttagtc tggaaatggc
     2221 ttttgctatc gtggttggta tttatttatg gaataataaa ttcagctttg gtttgcgcaa
     2281 cgaaaaatat atattgggag gaggtatttt atttcttacc atttttattc acgtatttgc
     2341 tgttcgttca ggtatattaa ccatgtatat tgcttttatt gtgatgatat tgtattggct
     2401 atttacaaaa aaagcgtatt tgcaaggctt agttgcgtta atagctttag tgggatttgc
     2461 tttcagtgcc gttatgtaca ttccctcatt acatgctcgt atgttatatt ttaaatacga
     2521 aatggattta attaaaaagg gggagttaca ccccgaacac agcgatgcac agcggttgtt
     2581 aagtatacaa tacggtgttc agattgcaga agaaaaccca ttacttggag ttggtgtcgg
     2641 cgatataaaa accgaaatgg ataaattata taaccaaagt gccggcagcg aatttgtaaa
     2701 atctaaattg ccgcataatc aatttgtgta ttttttagcc gcaagcggta tcattggatt
     2761 tttagtattc atgttgtctg tattatatcc ttggtttagt cataaacgat ttaaaaacat
     2821 gttatattcc gtgtttattt taattatgtt aattagtttt atggcagaac atacattaga
     2881 aattcaaatc ggaactgcat tttatctgtt atttttgttg ctcataaaaa aatatatcga
     2941 cgataccgct caacttgctg aaagcgaaaa acaatatgca taaagtaact gccatcatac
     3001 catgcaaaaa tgaagcgcac aatattgaag cggtactgga gtctgtaaaa tggtgtgatg
     3061 aaattttagt agttgattca tttagtaccg acaatacaat tgagcttgca aaaaaatata
     3121 cgtcccgcat tattgaaagc gaatatatta attcagccag tcaaaaaaac agagctattc
     3181 cacaagcagc tcatgaatgg atattacttg ttgatgccga cgaacgtgta acacctgaat
     3241 taacaaatga aattcagcaa ttattacaaa gcgatgtgat accacacgat gcctattgga
     3301 tttaccgtaa aaattatttt atgggcaaag aggtaaaata cagtgggtgg cagcgcgata
     3361 aagtaattcg ttttttcaaa cgcgacactt gtcgttatga agaaaaacat gtgcatgcag
     3421 aagtaattac ctcaggctca gtaagtaaat taaaatacaa acttgttcat aatacctata
     3481 aagatatttt tcattatctc gaaaaatggg accgctattc cacctggagc gcaaacgatg
     3541 ctgctaaaaa agtaacccgc cctgggtttt atcattttat tatcaagccg ggttttcgtt
     3601 tttttaaaca ctacatcatc gaccttggtt ttttagacgg gtggacaggt tttattgttt
     3661 gttcattagc cgcaaaaggc gtgtttatgc gttatgtgaa gttgtatgga atgattaaat
     3721 ctaacaaaaa ttaattacca gtcatcgtca gggaaatcgc cgctcgacgg ttttgtatca
     3781 agtataaaat ttgttttgcc ggttaaatcc aatgcatatt cgataataaa tggaggcagt
     3841 tcattattag gtgttgacat tttagcaacc cacctaatac gcattctttt atgctcattg
     3901 agacctagta agtaagggac tgaaggtact ctatacacgt ttaatgcatt tacatttaac
     3961 cgtctgattg ttgttacact gtgaggaaga tcgttttggg gttcaaataa ttcaagttca
     4021 gcatattgct catcaattat tacattagcc cagggtgcaa tttgaaataa ccctattggg
     4081 gaaatggtaa ctaatgcatc aaacactact cctaattcat ctctggctat ccattctaat
     4141 tttatagttg caggtctcgg atctgttcca agtacctgcc cttgctctcc cgcaggtcga
     4201 aaaccaataa cccctattcg caatttattg caacgctgat aggtatgtgg ccatccggtt
     4261 ggaaaataaa caggaccatt attagtcatt tcagttactg ctttaaaagt accattcaaa
     4321 ttcacaacga aatctgctac cggcaataca aaaactgttc ttccggttat tttaccggta
     4381 atcgctccaa ttaactgaac atcataagat ggattttgat tttcatctcc ctgaaacaat
     4441 gcgtaaatac cacctgcagc ttttaccgca ctcataatat caagtccgcc ggtggcgacg
     4501 gcagtagaaa taaaaccacc aataattgaa gcaccttgga tagccttttt tgcataggca
     4561 acatattctg catcaattcc taaactactt gttgatgatt cctccgcaac aggttttgca
     4621 gttccgctcg cggttaattc caggtctgca gttaaatttg attgtccttc taaaatgcca
     4681 ttaaaatcta ttttaaataa ccttctgtct cgctcctgta gttcagaagt aggcgccttt
     4741 acaccaggga acaacaatcc taaacaacca cccagtccac gtaatatttg agtttccgga
     4801 tataatgcag gatcaaataa tgttgctatt tctgtccgca accatttacc gggagcggaa
     4861 gcacttgatg ctgttaaatc gtgaaaagcc atttctaatc ccggccaaat aaatgctgaa
     4921 cctcctggat aaccggtaac ttcctgtaca ttatctgcag ttggagtatc acttgtttga
     4981 aaacttttta tttcgggtgg tacatccatt aatggaaaat tccaggtttg tgatgctatt
     5041 ctgtctgcat taacaagtga aaatgttgca attaatttat taaaatttcg agattctaca
     5101 ttcggtaaat aaacaaataa tcgcataaca ccggtaaata cattataaca taataaataa
     5161 ccgatttgtc ccggagaacc accaaattga taaccaaata cacgccaacc tttatttgga
     5221 tcaggatacc aataatcctg aacttcattc ggaccgttaa taaaattatc gggacttgcc
     5281 caccatggtc gtgtaattat ttcgtggtca aaatcaccgg cagcatcgta ccaaaaccaa
     5341 tctacaattg gttctgttgc cagaggatca gggatgcccg aaattgtccc aacctgaacg
     5401 cagggtaatg tttcgtctaa tgggtcaact acaatttcac catctgctgc aataatatta
     5461 tttacattat ttataattgg ctgcccattt agtttattag ccttcctaaa catagtaacc
     5521 gcgtttccat aatttattcc gggatgttgt aaatatttat tgagttgcaa aatttcattc
     5581 tccagttcaa tattttgaag ttcgctgcgt aataatttta tatgattttt tttatttaac
     5641 tgcttcaggt cagttatgcc aatttttttt aaggtttgaa aaatatttcc gggaatagga
     5701 ctaaatgcaa ttttattagg ttttaaaatc ggctgcgcaa caattgctgt agatgatggt
     5761 ttaaatatta cggcatcccg aaaaatatca acttcctgaa aattttcaat caccatcaaa
     5821 taattgccat aagcaaccga tataggttct ttaaaataat taccatttac caaaacagga
     5881 tatggagttt gaatgggtaa tttatataca tgcactttac acttttcagc gcctacaggg
     5941 aaattaaact caacaacgcc ggccttttca acccaaaaat gaaaaccaat aacagaagtt
     6001 aggggaacat tttttttaag acgtattata ttaccctcaa catagccggc atcaacaaaa
     6061 gcaatgctat tttcttgtaa gtgtaatgtg ttcataggaa taaccttttt ttcaaaggta
     6121 acaggattaa tgatatgaac attcaccgaa aaccgtgaat ttgaatgttt aatttgcttc
     6181 agttatagca gatttgtgga tgaagcaata atgcttattt caaatttaaa caaaaaaaca
     6241 ggctgatttt tatgttgtga gaatcacttc aaaaacttag caacataaat ttcctgacgc
     6301 tgacttattc gtaaataata aatccctctg gcaaggtcgc ctacatttat ggtatagtct
     6361 tttatgggtg ggtagaattc taaattataa ctcagcacta cttgacctat ggaattataa
     6421 atcgccattt caatttcttt gtctcgcgta ctggttacct gtaaataaat attatctaca
     6481 acaggatttg gataaatatc cactattact aattcacctt cgttggcaac tgcacattta
     6541 tattttccca acacgcgata gatatcttta gatgtaagtg tattacacat gatatctttc
     6601 ataaatggtg cggcatataa aataattgaa tcgggaataa ctgcatcgtg tcctgctcct
     6661 tcaccaatat aaaattgaac agttctgcca agtcgcttcg atagttgtgt taattgccaa
     6721 ctgccatgta aattaggata ttcgaaaggt tgataacaga aaaatgttgg gcctgaaaaa
     6781 aatggaacaa tagaatcaca agtaccatga aaatataaat gcggataatt gttattatat
     6841 aataaactgg tatcttctat tccaccggct tctgtaatta caccaatggg attaaagtga
     6901 taattttcat aatcatttac cgccgtgtca atactgccta attcatcata aaattgagta
     6961 ttgtaatcgg actgacttgc atacaaggca aataatatta atcgtgaacc agcactgttt
     7021 ccttcaacaa atattacatt agtatctata ccataaacat cggcctcacc ttttataaaa
     7081 cgatatgccg ctcttacgtc ctgcactgcc ctgtaagtag ctctgaataa atcggtagta
     7141 tcgccgccgc aaccatcgtc agcatcgcca ttatcccagc ctatgcgata gtctatactc
     7201 acatacacat aacccatttt agcgtataaa tttcctaggt caacggcgcc ctgactatct
     7261 tttgaaccac caaccaatcc accaccgtga acgaaaaaaa ttaatggccg tttgctgagt
     7321 tcatcaaatg cagtcttcgg tttataaatg tccaaaaata aattctgatt attacccaaa
     7381 ttatttactg cttcaccgta tttaacattc gctaaaattt ctatttcatt atcatcaaat
     7441 acaaattcat caaaccggga catcgtacaa taatcctgag ctgaaaggaa taaagaaaca
     7501 attaaaaaaa atacggtaaa aaatgttttc atcatattac actaaacaaa cagtacaaat
     7561 ttacggcaat taaaacaaac tatcttcagc ccagtcagac ctttcaggaa aatcggtggt
     7621 gtaatgcaaa ccacggctct cttttctggc actggcgccg cgggtgatta agaacccaat
     7681 tgtgattaaa ttccgcaact cacacaattg tggtgaaata gcagtggtat cgtataaacg
     7741 ctcggtttct tcatataata aatgcaaacg gttaagggct cgttcaaggc gttcgttgct
     7801 gcgtacaatg ccaacataat tggtcattat ttcttttaac tccctgatac tctgcgttat
     7861 cattaccatt tccttgggct cattggtgcc ttctgcattc caatccggaa tattttcctg
     7921 aaattcaatg ttatcaattt ttgcagctgc atcttcatat atacgatttg caaataccaa
     7981 tgcttccaac aatgaattac tggccaatcg gttagctcca tgtaaaccgg ttgaactaca
     8041 ttcgccgcag gcatataaat tattaatact ggaattgcca tgtgcatcgg ttaaaatgcc
     8101 accacacata taatgtgctg caggaactac cggaataaaa tcttccagaa tatctaatcc
     8161 gatagattta catttttcat aaatattcgg aaaatgattt aataactctt cttttggaat
     8221 aggcgcacaa tctaaataca caaattcatc acccctaatt ttcatttcat tatcaattgc
     8281 tcttgcaact atatctctcg gtgccaacga tttacgttca tcatatttat gcataaacgg
     8341 tttaccgtca cttgttttta attcacctcc tgcacctctg attgcttctg taattaaaaa
     8401 tgaaggcgat actccgggtt gatataatga tgttggatga aattgcacaa attccatatt
     8461 cgaaattctg cctttagccc tatacaccat tgccatacca tcgccagttg caatgagtgg
     8521 attggtcgta tttttataca cctgacccgc accaccgcta gccattactg taattttggc
     8581 tattattttt tcaatggttt tatttttttt attcaacaca taaacaccgt aacattcaat
     8641 atcaggtgta tgtcgtgtaa ctattcgccc aaggtgatgt tgcgtaataa tatcaattac
     8701 aaaatggtgt gcatgaatac tgatattagg taatgaaagt gcttcttcta ataatgtttt
     8761 ttcaatttct ttacccgtta catctttatg gtgcaacaca cgaaattctg aatgtcctcc
     8821 ttcttttcct aaactgtatt cgccttcttt atttttatca aaatcggctc cccaattaat
     8881 gatttcttta acccgttcag gaccttcttt cacaactatt tcaacaatat cgcgtttaca
     8941 caatccatca cctgcaatta aggtatcgtc aatatgtttg tcgtaactat ccttttcaaa
     9001 atcccaaacc gctgcaatac caccttgtgc gtatttggta ttggtttctt cttcttcagc
     9061 tttggtaata attgtaattg ttttatcggg aaatctgcgc gctgttttta gcgcaaatgt
     9121 taaaccggca ataccggaac ctataacgag gaaatctgct ttatgcataa tgcgaaggta
     9181 cggtgtttat accaatgtga aaagtgaata atcccgatta cggaaaattg atacaattag
     9241 attaagatga atttgaaaaa atactaaaaa taacagtcag aatattttct atttattagt
     9301 ttgtttataa gcatatgttg cttctaacgc ttcataatat tcctcaccat atttgcgaat
     9361 aagcgctgtt tttacaaatc ggaaaattgg cacacctaat tccttgccat ttttacacgc
     9421 cggtttacaa atattccatt tttcgtaatt caacgcatca aactcaccta cttttgaaat
     9481 acggatggga tataaatgac agctcaccgg tttttgccat tttacttttt tatctaaaaa
     9541 cgctttttct atgccacaca atgcaatacc tttttcccaa atggtgtatg cacaagggcc
     9601 atctttcatt aatggtgttt ttaaaacccc ttcatcgttg gtcacccaat aaccttgttt
     9661 ttcgatggca tcaattcctt ctttggtgag atattctttc actaccggat acactttttt
     9721 caacatcttt ttttcatcct catcaacaaa tgcgccttca tcgccttcta cacaacaagc
     9781 gcccatacag gcatttaaat tgcatacaaa ttctttgtcg agaatgtctt cgcttactaa
     9841 agtctgttga attacaatca tgttgcaaaa ttaactattt tttgaaaagg atggatgggt
     9901 taatctctta ctctttttac atccccagta aaaatatagc gggaattttt tctaaagccg
     9961 gcatatgttt tttccaatac gaaatgggtt gtgtgcgtat tgattctttt gtccctgtta
    10021 aatcataagc tatacacaat aaaacatcac tactgcacgc acttaaaata tcctgtaaaa
    10081 tggcattatt gcgataaggt gtttcaataa atatttgtgt tacccctttt tttgtttccg
    10141 cttccatgtg ttgaatatat tgtttgcgct gaggttgttt taccggaata tatccatgaa
    10201 aatgaaactg ctgaccgttt agtccgctac ccattaacgc taacaaaatt gaagaaggcc
    10261 caacaagcgg aactacccga atattatttt ggtgacaaaa tcgcaccaat acacttcccg
    10321 gatcagcaac agccggaaca cccgcttcac tcattaatgc acaatctgtc ccttgtttta
    10381 aatactccat gtaagttgaa acagttgccg catcggtatg ctcattaagc ggtaaaattg
    10441 gtgttgtttc aaacgaagcc gtgaatccag atttgcgcaa atatcgcctt gccgtttttt
    10501 cattttcaac aaaaaaaaca gtcaacttat tcgtgatatc aaaaacatat tgtggaatta
    10561 catgaagcgc ttcttcactt aatatggttg gtactaaaaa tagggttcca tgcataatat
    10621 tattatttta ctatgtagca gttcattcat tcgaatatat ttgcatactg aagtccacaa
    10681 caaagttgaa tgaataattt cacattaaaa aaaagttttt acacgcattc cgatactttg
    10741 ggcattgccc gggctttgtt gggcaaaaaa ttaatgacct cttttgaagg taaggttacc
    10801 gctggaatta tttgtgaaac tgaggcctac tgtggtgttg aggaccgtgg atgccatgcc
    10861 tacaatggcc gctttacgga aagaaccaaa attatgtatg cagaaggtgg tgtgagttat
    10921 gtgtatttat gttacggcgt gcattatttg tttaacgttg tcacccacgt tgccggtcaa
    10981 ccacatgcag tgttggtacg ggctattgaa cctactgaag gtattcctac aatgcttgaa
    11041 agaagaaacc taaagcaatt tactcccggt ataggcgcag gccccggttt ggtaacacaa
    11101 tgtttgggat taacaacaaa tcacaacgga ctttcgttat tgggtcagga catccggatt
    11161 tgtgatgctg caccaattaa cccgaaacaa attgtagctt caccacgtgt gggaatgaat
    11221 tttagtggcc cttataaatt aattccatgg cgttttagaa tagccaattc gccatttacc
    11281 tccaaagcaa aataagttgc ggaaaaaatt aaatactgta attgttgtat tgcgtactac
    11341 cgaatggaag ctggtattta aaccttttgg gtatcataaa ttattattgg cattattcgg
    11401 atttttttta acaggaagta ctagtggttt aattgcaggt ttaattattg ggataattat
    11461 ggactgcgaa attattttta aagaaaaacc ttcaaaaaaa tccaccgaca acagaataag
    11521 ttatttaatg ttggcagctt ttgttttaca ggccggtgaa ttaaatggga gaatttcgaa
    11581 tgaaacatta catcgcagac ttgttaataa atttggggaa gtatatgcta caaaacgatt
    11641 tgcttttttt catgaactat ttcgtcagcg tattcaggta gaagctattt gcgaacaaat
    11701 taaattacat gccgatttaa aagataaaaa agatataatt ttatttttat ttgaaatcag
    11761 ctatcaccca tctgcaccaa aagaaaaagt aacacacacg attaattatt tagcggctcg
    11821 gattaatttg ccatatgaag atgtaaaaac aatttaccgg acattttcgc aacaacatca
    11881 aaacaattat agtagcaata caaaaacgta tactgaaaaa acgactccaa aatcagatat
    11941 atacacatta tttaatttga ccagcaactg cacagaaaaa gaattaaaaa ctgcatttca
    12001 ccatctggcc aaaaaatatc accccgattc aaatccaaat gccagcaacg ctgaaaaaat
    12061 aacaatgcag gaaaaattgc gcaccataat tgaatcttat gatgaaatta aagtacgccg
    12121 cgggtggaaa tgaagtggtg agtctcatgc tatataattt ttggtattgc ttattagatt
    12181 acctttctgc acaaacactc tttcccacca caaaggctag aaggtgctca aagttacgcg
    12241 aagggatgta ggataaactt gcagcatttt tgtagtaagg acacatgtca tttgtcccta
    12301 caacaaaaat tgcttattaa atctaacatt aaatttcttt caatttaatt gtttgccaat
    12361 aagtgaaatt taatattcaa agttaagtca ggcaccaatg tagtggcgaa ttgcatccgc
    12421 ctctgcattg gtgtctgacg attggtgtat aaaaatacct attgtatcaa attttaaaat
    12481 agagataaaa gggctactac tcatcaccca tgtccgtcat agcgggcaat actgatagaa
    12541 ttattttagt ccgcaattcc gataaaccgg aatacaaaat gaatgctttc ggaattttgg
    12601 gttatggctg ttttatgaaa tttatttttt ggggaaatat aacaagtaca cactttaggt
    12661 aatttaatgc ttatagcatt gactccggtc ggaagaccgg ggtggcgtct ggagtcaaag
    12721 actccacgac aacgttgtct gcgacaacta ggggctttac gattgaatta taaaaattcc
    12781 ggaggaatta atatttataa taaattcaag ggcacaaact ggcatattgc catcactcat
    12841 tactcatcac tcatgtccgc ctcggcggac aactcattat ttcgggaata aaaaagttag
    12901 gataaaacca taaaaaaaac ccccagctca gtcgggagac tttaccgggg gatccccatg
    12961 aaaacacaaa gtttcgtccg ccgaagcaga caatatttta attactgcgc aagatacgct
    13021 ttcagtgctt tatttctagt acttgaacgc aatttattta ttgcattatc ttttatttga
    13081 cgaacacgtt cacgtgttaa tccaaactct tcaccaatat cttctaaagt catggcgtaa
    13141 ggaacaccaa ttccgaagaa taatttaatt acatcacgtt cacgttcagt aagtgttgct
    13201 aacgtacgtt cagtttccac ttttaacgag tgactataat ctaatttttc atctgtacgt
    13261 tcagtgtcac gattttctaa tacgtcgagt aagctgctgt tttcaccatc atcaaacgga
    13321 gcatcaacag atacgtggcg acttgaaaca ctcaaagtat ttttaatttc ttcaggtgcc
    13381 aattctaaca cttcagctaa ttcttcagac gtaggttcac gttcgaattt ttgctctaat
    13441 tcagaaaatg cgcggttaat tttactcaaa gaacctactt tgttcaatgg taaacgaact
    13501 aagcggcttt gctcggctaa tgcctgcata atgctctgac gaatccacca aacggcatat
    13561 gaaatgaatt tgaaaccttt tgtttcatca aatttttgag ctgcttttac cagaccaacg
    13621 ttaccttcgt taattaaatc gttcaacgac aaaccattgt gctgatattg tttagctacc
    13681 gaaaccacga aacgaaggtt ggctttcacc aatttctcca atgcaagctg gtcgccttgt
    13741 ttaatctttt gcgcaagggt aacttcctcc tctgctgtaa ccaggtctac cttaccaatt
    13801 tcctgtaaat acttctctaa cgactcgctt tcgcggttgg tgatggattt cgtaatcttt
    13861 agctgacgca ttgttaatta acctcgttta tttcttgtga attgaatggt tcgttaaatt
    13921 agaacggtat gctaattaaa aagtttcctg aaactcaata aaaattaaaa attgagccgg
    13981 ggtgcaaatc tactaagatt ttcgatacaa atcaatagca gaccgttttt acctgtaaaa
    14041 cgataatttt aacgctttcg gtattcatac atggtttatt caccttcagg caattgtaag
    14101 gggtaattgt cattgttggc aagggcttta ctgcgtatat ttgaacatct tatgcggaaa
    14161 tggttgctga tttatgcgct ttttagcggc tttagtgcct attcacaagc cttttatgaa
    14221 ggtccttcgt ggacggactt cggcgatttg acctatactg ccttcggttg gagcatgagt
    14281 aatgccggtg atgtgaatgg tgatggcttt gaggatatga ttgtttccgc tatcgactac
    14341 agcaaccctg aggaaacaaa tggcgaggaa ggcaaactgg cgctttacta cggcggacca
    14401 gacgggcttg aaaccgaacc cgcatgggag tttgagtcta ataacgatag ttctgtgttg
    14461 ggttttagca cttccggtgg cgatttgaac ggagatggat ttagtgatgt ggttgtaggc
    14521 tgtttgcaat ggacagggga agaatacaat gaaggacgta tttacttatg gtacggctca
    14581 gaaagcggtt taaacgcggg tgagccggac tggagcctcg attttgatca ggtttttgcc
    14641 cttttaggca gtggggtggc tttggatggg gatattaatg gcgatgggta taacgacctg
    14701 tttttatccg ccaaaatgtg ggacgaaccc gaaatagaag aaggcaaaac atggctatac
    14761 tggggcagtc ccgacggccc cgttgaatcc ggatggagct ggcaggctaa tcaggaaggt
    14821 gcaatctcgg gttttccggt aaattatgcg ggagatatca atgccgatgg atttgatgat
    14881 gttataattg gggctaatca atacgatttt gatgaaattg acgatggctt agcagttgtt
    14941 ttttatggca gcgaaaccgg gcttgcagct gaaccggatt ggatgatgag cagcggtcaa
    15001 aaaaaatgca attttggtca ctgggtcgat ggcgcaggtg atgtaaatgg tgatggatat
    15061 gacgatgtgg ttgtttcggc acttttatat gaaagcgact cggcaagtca taatgaaggc
    15121 cgcatttttg tctaccacgg ttcagctaca ggtttgtcaa acgaattaaa ctggttcgga
    15181 gaaattaatc aggccgaagc gcaatttgga tacagttgtg ccggtgcagg cgatattaat
    15241 aacgatggtt ttgatgatgt aattggtggt gcaaaatatt ggaccaacgg agaagcaaat
    15301 gaaggtggcg cgtttgtttg gtttggaagt ccgacaggac tcgaaattga ttattgtttt
    15361 tctgcagaag gtaatcaggc ggagggttat ttcgggcgac atgttggtgg agatgctgat
    15421 tataacaatg atggttattc cgattttttt gttggtgctt accgctattc cgaaatatta
    15481 gcagccgatg gaaaagcatt tgcttataat ggtgccactc gacctgcaga tttttatttt
    15541 gaagaagata ctttttgtat tgatgatgta aatccattgc caataattac cggatttacg
    15601 ggaggtactt tttcttctgc tgatgttgtt gtagaagcaa tatccggtga aattcattta
    15661 atggaaagcg gtttcggcgg accatttatt attacttata ccgttgatgg ttattgctcg
    15721 gtaaataaaa caatttggat acaagatgca aatgccctta cattgtttaa ttatgaaaat
    15781 gatagtttgt gtttaaatga tgcaataata attcctgaaa taattgttcc atcaacagga
    15841 acattttatt cggatgatat tattgttgat gtaattaacg gggcaatcaa tactacagat
    15901 gttgccgcag gttgggcgac aatttattat gccggttcta ctgcggccgg ttgtaatttt
    15961 attgactcaa tagccctata ttttcaggag caagtaacta ttaatttgga tcaggataca
    16021 ttttgtattg atttaggtga agcaattcct ttagtaaata ttccgggcgg taactatttt
    16081 tcacctgatg caatagtgga tgttttaaca ggaacaattg atttaccttc taccggtgtt
    16141 ggcggcccgt atcaaattta ttataacgta aatgccattt gtcccgacgc atctttcatt
    16201 ttctacatcg atagtgcaga taataattta gttgcatttc attttgaaga cgatactttt
    16261 tgtttatacg atttaaatcc ttctccagtt attactggct tagccggggg aatattttca
    16321 tcggctacag caatattaaa cgacatcacc ggtgaagtca atataattgc aacaggagca
    16381 ggaacacatg tcatcttata tcaacttaca tccccaaccg gatgttcgct tatgtttact
    16441 gatagcattg cgattaataa tccgccaata gttgctataa cttatcctat agacaccctt
    16501 tttattggag atgaaaatgt tactcctgaa atattaggat taacaggtgt aatcaatgct
    16561 attcccgatg gagttgtgtt tgccgatgac ttcggaacga ttgatttaat taattcaaca
    16621 ccgggaaatt atagtattta tataacggta gaagaaaatg gttgttataa ttcagatagc
    16681 gcaacaataa caatattacc ggcatgtgag gcgccgcaaa atattgttgt tgattattac
    16741 aatagcaaca gtgtcattat tagttgggat gaacatgatt tttataccga ctatacaatt
    16801 ttagttattc tcggcgatga caccttaact tattacgcca ctgaaaacac catcactatc
    16861 accgatttat tacctgatac aacttatacc ctagtggtga ttacgaattg ctcatcgtca
    16921 cataacaatg caggtaatcc actagtgata acaacagcag cgttagcaat tgaagataat
    16981 ttagggttta atatgctaat ttttcccaat cctacatcaa gtgattttac aatttcaact
    17041 ccgcttttat taaatggttg taagctttcc atatttaatg tggaaggaaa acaggtgtat
    17101 ttggaaactc aactgccaaa acaaaataca tttcttatca cgtcccatca acttatctct
    17161 ggactatatt tcattcattt aactaacggc aatcaaacaa cattttcaac aattgtaata
    17221 gaataaaaaa agcctcaatt acgaggcttt aaatgattta gtttattctt ttttacaaca
    17281 actatgtaat tcttcataag cctttgcatc agccggcact tcatcggcat catatccaat
    17341 agctgttatt gctgtcttaa ttgctgcagc atttgttttt tcactatcat atttaacacc
    17401 tattacctta gtgtccaaat ctaaatctgc caatttaact ccttcaattg caccaacagc
    17461 tttttcaatt gccgctttac aagtggcaca ttgtgctgaa gtttttattt caatatattg
    17521 tttgctttga gccgataaac ttcctgttat gataaacagg ctaagcatca agattatttt
    17581 tttcataatt acaaatttat taattaatta aatattaaac gtattcctcc gtaaaattta
    17641 cgttccatta gtggtgcata aattacagag gcatcaaaat cctcgccaaa aggattggtt
    17701 gcgccaatta taggttcatg ttgcgtgtaa ttgccaatat tttcagagcc cgcataaatt
    17761 tccagctttt tgaactgttt ggttatttgc cccataaaca atgtatacgc cggtgaagtt
    17821 gtccccaaat catgtgcctc atggttatcg cttaaatttg gcaaacggct tgttccataa
    17881 aattgtgatg tggcatcaaa aagtaaacca gcgcgtttcc actcataact caaatttatt
    17941 aaacctctgt gtttataggt gagaggcacc tctaataatt caccattaaa tgtttcttgt
    18001 gcatcaatat acttgtaggc ggttctaatt tcaaatcttt ttaataattc ataatttatt
    18061 tctgcttgca aaacattgct ataagaatca ccttttaaat tataaatata aattgaatcg
    18121 tcatcagaat caatatctac tacaacctga ttcacaaaat cggtgcggta aacatctaat
    18181 gtaaaagtac cctctctaaa gttgagttta aatggttgaa ttacagtaaa cccataattc
    18241 catccctgct ccggcaataa cgattcggtt attaccaaac tacggttgct ggtaagtaat
    18301 gtggtatttt ctgcaaaaac atttgccgtc cgataacctt ttcctattga agcccgaatt
    18361 gttgttgact taaacgcatt gtatttcaca tgcaaacgtg gagtaataaa tgtgccatat
    18421 aaattgtgct ggtctccacg aacacccaca agtatactca acttatcatc gggtttatat
    18481 tgatactcag caaatattcc gggaacagat tctttgcgca aataatttat agaatcaaac
    18541 acctcatcaa aatcgttata taaataactc gctccggttt ttatggtatg attggtattt
    18601 aaaataaacg actgaaaaat aaaattagca gatgcatata cctctttacc gttatagcgg
    18661 ttcaaaccaa acaacccatt ctgttcatgt tgcgcaaaat tcacgatggt tccaattgaa
    18721 gtgtttgggc gatcaaatac aaatccgttt ttcataaatg cctcaaatcg attgatattt
    18781 acttctactc cataaccatt gttaaccgag ggctcaagcg atttatcata attgatctga
    18841 ccaccagtta aatcactttt aacatattta aatccaaact gcgcttcgtg aatattatcg
    18901 gaaaaataat tccatttgtt cataacagcg taagtcgaat tgagtggagc atctaaaaat
    18961 gtatcaccat tgtggtccag tttgttttgc agctgactgg tatgcgtgta aagtgcagtt
    19021 gcccattttt cattaaacag atgtttaaaa ttaacattgg cttcatatct gccggagtta
    19081 ctggcaaata aattaatcaa ccaaatttca tcatctatat cataaggctt tttaatttct
    19141 gtatttattg ctcctgtaat gccctcatat ccattcacaa ctgagccggg gccttttgta
    19201 atttgaatac tttcaatcca cggaccggga ataaatgtta agccataact cgatgccaac
    19261 ccacgaacat taggcatatt ttcaaccatg atttgagaat aaaccccatc taatcccaac
    19321 aatttaattg tttttgcacc ggtaatcgca tcactaaatt cagcatctac ggtgttgttg
    19381 gtttcaaaac tttcactcag attacaacat gctgctttta ataattcttt tctgtttagt
    19441 gtttccgtat ttatggtatt gatggtattg attgttcttc ctccggtttc accttcaatt
    19501 gtaacaactg ctaaatcggc aattggtgtt aacattacat gcaacgaatt attgttttta
    19561 gtatctaatg tatcattttc ataccctaca taacttacaa caataaattg ttggttatta
    19621 tccgtacgat ttaatgtaaa aaatcccaat tcattagaaa ctgttcccgt agtggtgttc
    19681 aaccatacaa tgttggcccc gggcaaacca accattttac cttgttcatc tgtgctcata
    19741 acatggccgg aaacatcttg tgaaaaagat attttgctta agcctaaaag caccaataat
    19801 atataattat aatatttcat ttgagtataa tttaaaataa atgactaaaa tgttcttcaa
    19861 gagctgaaaa acacaattac actaaaatga aaatcaaatc tgaagcgtcc tgatgtcgga
    19921 caaaatttct cgccctgatt taggcggtgg ttgcggagaa taattggtta atgctacaat
    19981 acttgtttca gaggcagtaa atgattgaaa taaaaacgtt gccattaaaa caaaactatt
    20041 gtcgtcagaa cgttgaactg atgcctgata atctaatttg taaaaattgg tttcatcatc
    20101 acagcaatct gaagttaaag atgttatatc tgaaacctgg tcaatcggac aacaagaatc
    20161 tattttagaa gcagaaactg cttgctgtcc atccataaaa cacatgtgtg aaaacatggt
    20221 aaaccccgtt gatgtagtaa ataataccac catcattaaa taggcaccaa aacgtttaaa
    20281 caccttgttc attagtacaa aattaccatt tttatgtcaa attgacctct tttagcttgt
    20341 caggcatatc ataagtcacc atttactacg ttataacggc tatctttgct ataaagtttt
    20401 gaacttaaac aaaaagtaag catgaaaatg aaaaaatctg ttttcctcct caccttaacc
    20461 accattagtg ctgttatgtt ccttagttca tgtgctaaaa aagaagggtg catggaccag
    20521 gcagcattga actatgaccc tgaagctgag gtagataagg gttgtgagta tgccagcgac
    20581 gatgatgtag ctgtaacctt gcatatgcat caaaatattg ggggcggaga gcttgttgaa
    20641 gagactgcct atactataaa tggagtagtt actaatttga atttagtaca gttttatgtt
    20701 tcagccatta ctttagttga tgctgcaggt aacgaaatca cgaacgatga cgtttatttg
    20761 ttagtgaaac ctgaagaaga agcttatgct ataggcaatt tccctgccgg cgattatacc
    20821 cagattaggt ttgatattgg tatagattcc gttaccaacc atgccgatcc cagtaaatat
    20881 gacattggca atcccttagg tgctcaattc ccagctatgc attggggatg gagtttcggt
    20941 tatatttttg ttcgtatcga cggcgaagct gatgccgatg gcaatggcgt acctgacccg
    21001 gatggccaat ttgaaatgca tttaggaagt gacgcttatg tagccacaat tgaaattgat
    21061 ttacctatta ctatcgcaga aggaaatgaa aatattgtac accttgatgc taattgggat
    21121 acttttttta cgggtgttga tatggcaaat gacaatacaa cacatgtaac agataatgct
    21181 gctctttcaa gcttgttata tgaaaacatt tttaatatgt tttctgctga agaataaatg
    21241 aaaaaacaga atacaacata ttatatatgt ttattctttg cgggaattat ttttagttct
    21301 tgcgcaaaag acccaaaaat tattgttgaa ccggatgcgt atgtgttaaa cgtaccatcc
    21361 ggttttgttt ttccaaatat tcctgaggat aatcctttaa ccacagccaa aattgcttta
    21421 ggaaagaaat tattttatga aaaagcattg tctgtcgact ctaccatttc atgtggaaca
    21481 tgtcataaac aggaatttgc tttttccgat aatgttgctg ttacggccgg tgtagaaggc
    21541 cgtctgggat ttagaaatgc acccacctta accaatatag cctggtcgcc tgaaatgttg
    21601 atggatggtg ggaatccgac actagaaaca caggtgtatg tgcctattga aacacatttc
    21661 gaaatggatt ttaatatggt attattggta aacagattaa atgctgatga tgaatatgta
    21721 gatgcattta ataatgtgtt tggcacaaac ccggacccat ttggaataac acgtgcatta
    21781 gccgctttcg aaagaacgat aataagtggt aatacacgtt ttgatcaata ttattatcaa
    21841 gggaaaacgg aagtgcttaa tgaacaggaa ataaatggta tgcaattatt ttttagtccg
    21901 acactaaaat gtgcttcctg tcatagtggt tatttattta ctgatttcac gtatcaaaac
    21961 aatggttttt ttgctgatta cagtgcagat tccggtcgtg ccagaatatc gttattaagt
    22021 gaagatgtcg gtaaatttaa agtgccaaca ttacgcaata ttgcattaac cggtccgtat
    22081 atgcataacg gacaaattaa tagtctcgaa gcaattattg atagttatgc caatggaggc
    22141 agtggtcatg ttaatcagag cgaattaatt cagggatttg ttttaaccgc ccaggaaaaa
    22201 gaaaatttaa tattgttttt aaatacatta actgataatc aattcattaa taatccggac
    22261 ttaggtgaag attgaattat tttgatgata tttttgtttt ttatctaact ttacttacaa
    22321 atgaaatggc gtagcttaac aatattgttt tttagttttt ttgttatact ttttactcag
    22381 tgtaaaatag accccgaaat tataagcggc gaagaatggg tgtacgaccc aacgccgtat
    22441 gcggttatgt cgcctatggg cattccaaat gtgccaaata ccaatggtaa tcctttgacc
    22501 gcaaaaggtg ttgaattagg aagatattta ttttatgacc ccattttatc tgctgatagc
    22561 tcactatcgt gcgcaggttg ccatagtgtg aaccatgcat tttctgatac ggttaagttt
    22621 agtattggta tagatggaat tgccggaaaa agaaatgcaa tgcccatttt taatttggga
    22681 tttcaaccat atgataacgg atttttttgg gatggacgtg caacaacctt agaagaccaa
    22741 gcgttaatgc caattcagga cccggttgaa atgcatgaaa tattacctaa tgtaataaca
    22801 aaattatcgc gttcagatat ttatcctaaa atgttttatg aagcatttgg cgaatcgaaa
    22861 attactgcag acttaacagg aaaagcttta gcacaatttg taaaatcgtt gttagcaggt
    22921 aactctaaat ttgatagtgt tgtagcagga tttgcatttt ttactgacga cgaattagcc
    22981 ggttttttat catttgaatc cctggaaggt ggcgattgtt ttcactgcca cggtgtaaat
    23041 ggtggattgt tttcagatta tatttatcgc aataatggta ttgaccttgc aaacagatat
    23101 tttgattttg ccgatgccgg ttacggttta attaccggag acacttccga ttatgggaaa
    23161 tttaaaacac cctcattacg caatatcgaa ttaacagcgc catatatgca cgacgggcgt
    23221 ttcgccactt tagaagaagt attggattat tattcagaag gtgtaaacga cacacagttt
    23281 accgataatt ttatgcaatt tgcatttcag ggaggtgttc aattagatgc aacagaaaaa
    23341 gcacaattaa ttgcattttt aaaaacactt acggaccata cactaaagga taatcctatt
    23401 tatcaaaatc cgtttgaaga agattaattt tattttgcaa tagtcacagt gtcgacaaac
    23461 tgttgcattt cttttaatac atcatctact aattctttat tgaaggcatc aacaggtgga
    23521 gtgtaattaa aatccaccaa ccatgtttca ttttctttaa ttaataaagt ttccattgta
    23581 gtactgtcta acgatgtcca tatatgtata aaggcagagt cgttaccaat tttagattta
    23641 tctgcatcta ctttataaat ataagttgtt ggtgttgctt cctgtacaat ttgtaaggca
    23701 ccttcaataa atttaaaatc gttgctcaat ttttctttac ttgctgcagt tactaattca
    23761 cttgcttttt ccaggttatg ttcattaaac aattggttat aaaattctaa agcaacattt
    23821 tccggtgttg atttttttcc ggagcaggaa ctgataataa ttgctgccaa caagaggaga
    23881 gggctgtttt tgagcgacat gtggttgaat tcagaacaaa ttaacgcaat gattggcaga
    23941 ttatgatttt tggggattca ctttaacatt tcaattagct aattagcaaa ttagctgatt
    24001 agctgatggg gtgatggggt gatgggtgga gtaatatagg aaggtgttta gatgtttcga
    24061 ctttgtgttg ctttgagcta cttggagact ttgtggcctg ttttcgaatg tagttcgtca
    24121 ccatttcatg caaatgtttc aaggcaatga acgtgttaat ctttcaattt cgcgcagtga
    24181 gcctcgaagt aacacgaagg atttaggcaa atcatttcat aactactaaa aaagtacagg
    24241 caattttcat catttaattt tcactcagag ccactcatca ctcatgtccg ccgaggcgga
    24301 caactcatta ctcattcgat gagaaatgag gaatgagtga tgagtgattt tggatgaact
    24361 tgacatttaa tatttgacac aacattttta gatagcatta caagcaatta ttaagactta
    24421 caagcctcga tacaccgctc attactcatg tgagctccgc gaacaactca tcattcataa
    24481 aaaaacccgg acaatgccgg gttttttata tcaatggttt aagatcaact gcatcctaag
    24541 ctttcgccac
//
LOCUS       DAORKW010000006        24436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_9_length_24436_cov_5.577048, whole genome shotgun sequence.
ACCESSION   DAORKW010000006 DAORKW010000000
VERSION     DAORKW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 24436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24436
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9_length_24436_cov_5.577048"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLB46_00625"
     CDS             <1..657
                     /locus_tag="PLB46_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF75042.1"
                     /translation="IIYWIYKLRLERALAVEKMRTKISRDLHDDLGSTLSSISILSGQ
                     VKSRLIKQPETVEPILDKINESAIKMNDSLQDLVWAVKPENDTLQELLVRMEQFASGI
                     LESKNINYTFTGPTTVSDIKLNPELIRNIYLIFKEAVNNAAKYSNCANVTITVKEKGK
                     QLSLSVIDDGIGFNPETVKRGNGLNNMTKRAAQIGGSLKIISEPNKQSGIVLECKITS
                     "
     gene            700..1338
                     /locus_tag="PLB46_00630"
     CDS             700..1338
                     /locus_tag="PLB46_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF75043.1"
                     /translation="MKVSFLMFEDNNELREALVTALNERNEVVCIGNYDNAIHATQHV
                     KELKPDVILMDIDMPGINGIEAVKQIKSQHVNVEIIMFTVFDENDKVFDAICAGATGY
                     LLKNSSTEKIAESIVDVANGGAPMSATIARKILFQFPQFKTAGKEKFNLSNREVEILN
                     YLVKGKSYKTIAQLCFISHDTVRTHIKHIYEKLQVHSAPEAVAIAMEHRLVN"
     gene            1415..1567
                     /locus_tag="PLB46_00635"
     CDS             1415..1567
                     /locus_tag="PLB46_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75044.1"
                     /translation="MKKLWYFIITWILISIPVFIFAQPQPQHNNSAITQTTNTNVLNE
                     VIIIVK"
     gene            1593..4250
                     /locus_tag="PLB46_00640"
     CDS             1593..4250
                     /locus_tag="PLB46_00640"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRF75045.1"
                     /translation="MKKIIILISLYCSVLITTAQNVALNIDGSAPDNSSMLDIKSTTK
                     GLLIPRMTQAQRTAIASPATGLLLFQTDLTKGFYYYDGAAWVFIGNSTAGWSTTGNSG
                     TTPGTNFLGTTDAKDLKIKVNNTVAGSIETSGNIFLGLNAGIVNTGANNTALGGNALK
                     ANTTGEQNTVIGTFAGELNTTGSFNVIIGEEALQNNSTTSGNVAIGYKSLEANATYGD
                     NTAVGYQSLMSNNAGYYNTAFGKSTLEDNTSGFLNVALGVDALANNTSGGWNAAGGAY
                     ALYANTTGSANTGFGYGAISTNTTGSNNSGLGNMALSFNSTGFSNSAVGSYALYANTT
                     GADNTAVGTSSMNQNAVGSNNTAVGSKALYYNTANNNTAVGTESLEGNTTGTANAALG
                     YRAMEDNTTGSNNTSVGDNALANNTTASNNTAMGSGALKLNTTGIKNTAIGTSTLTAN
                     ITGSENTAIGYNALAANTTSTGNVAVGANALQTATTGSSNTAVGYNALMNATIATQNT
                     AIGYQNLSALTSGSQNIAVGSNILTALTVSANNIGIGHNVLTSNVSGGLNIGIGFNIL
                     TSSTSSNNIGIGNNALAASTTGQQNIAIGMGALDQLTISSSNIGIGTNALGASTTGTG
                     NTAIGGSTMFNNTTGGSNVAIGNSALLTNTTGSSLTCVGENANTGGAALGNSSAIGAR
                     AQVNTSNSMVLGSVNGVNGAVSNVFVGIATNSPAHILELGVNDAAKPTSSTWTVVSDE
                     RLKENIKDFNDGLDLVMQINPIWFTYNGEAGLPHETGVGTIAQELQKIAPYMITKWTY
                     RESDTDQGMEYLGVDYGAMDFVLVNAIQELNNQVKQNSSAEEVAYNKQNNAIEQLQNE
                     NAALKKQLTEIEKQLNVLSDKMQASVAKK"
     gene            4255..5625
                     /locus_tag="PLB46_00645"
     CDS             4255..5625
                     /locus_tag="PLB46_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75046.1"
                     /translation="MVNKGLYGYSLLLFVFSTISSYGQAGLRLQTGAQLIINGSPEIV
                     LNNCGLYNDGDIDAGLGAFRITGTAATSNSVIAGSSYTTFYDLTLQKLSNDMQMEQHC
                     AITHFLTCDGGLLELNGYVIDLGTTGIIKVESEDTRVTGTSGGEIIATATLNAPSGAN
                     PGNLGASITSTANLGLTTVRRGHVQQTDPSGNYSIYRYYDISPTNNAGLNALLIQYYF
                     DAENAGLDENNFDHYLSDNAGATWNNLGQDARDPTDNFVKLSGYDAFARQTLADPIGS
                     PLPVVLIDFYAQCTLTSTVLNWSTASEINSDHFEIQQLDFNQNWVTISTIPAQGYASQ
                     TTNYTYSLPTSNTFYRLVLVSTNGDAEILEVIQSDCYIEPATAIYPNPTTDYLHIDFG
                     NHDNAQGQILIYDMHSQLVQQLLLQENSGKIQIDVRMFPTGIYLLEIQEGKNNKHFLF
                     NKQDKR"
     gene            5722..6315
                     /locus_tag="PLB46_00650"
     CDS             5722..6315
                     /locus_tag="PLB46_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF75047.1"
                     /translation="MQRLIDTIQKLEPLTNEQLKAIEQMRSIAHYKSLKKGDFIFTRG
                     DTPPISVFVAEGMLRHFVSDNNGNEKIIQFFMEGAFFDDCGSYVNNQPIDYYIQAIED
                     SEIIFFYLDELKQLAADSPLIEKIGTKIAADFLKNHQEHLTILMKYNPEERYKYLITN
                     KPEFVQRVSVTHLAQFLDVSRETLSRMRSRFAEQSIL"
     gene            6374..6904
                     /locus_tag="PLB46_00655"
     CDS             6374..6904
                     /locus_tag="PLB46_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRF75048.1"
                     /translation="MAIDIYQKLVLGKETFLPIKSDSDTFQMDWWMEPGGVVPQHIHR
                     YMDEHFEIISGTAIFIVDGKKITKNKNDELFIPKGVAHGIKNNSSEILNIKVTYTPAA
                     DTAHMFKIMAFLNQKYPGQASNMVKYFYMFPKLGLKPFSEIPSPFIMRTLAAILNMVG
                     FLAGWNKLVKAYKQTN"
     gene            7211..8203
                     /locus_tag="PLB46_00660"
     CDS             7211..8203
                     /locus_tag="PLB46_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75049.1"
                     /translation="MKKLFLLLFTSLFITDIYAQTNIYHALPDSGYYWNVIDLRTECE
                     TGSADTIKGLFKYATIQGDTLVGAKRYKKVHATNSLKEIVYFLGYYYEDTVNRKVYVV
                     TDNPDDHTITEESLLYDFNIGIGETVDDYYDFRGFDEDHPANSIVISVDSIEIESVYR
                     RRIGIQYELFQCDNKKEIKIDTTYWIEGIGNTAGFALTNQLPMNAGDYAVDVLACVSN
                     GAEVVFINPHEWHNCDSVFLKFIPIIDLNSEEIVTVYPQPADQLLNIELNLGSREGNF
                     TIQILDLSGKLVYRQVYFTADINIPVNNIPTGIYLIKIQYETGVYFSQKIIIQH"
     gene            8343..8696
                     /locus_tag="PLB46_00665"
     CDS             8343..8696
                     /locus_tag="PLB46_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRF75050.1"
                     /translation="MAYNEKLANRIRERFAELHPVEEKEMMGGLTFMYNGKMCVGVIK
                     DDLMCRIDPKIHNECVEKQGCKTMDFTSRPMIGYVIIDETGMKSKKDFDYWINLALAF
                     NPYAKSSKKKNSNKK"
     gene            8713..9972
                     /locus_tag="PLB46_00670"
     CDS             8713..9972
                     /locus_tag="PLB46_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75051.1"
                     /translation="MQLLKNYAITILLLLSVIGTNAQSFTPHPDNPILPLSFVNPGTW
                     NDPCVLKQGDTYWMYASSKNGVDIFDGEVVPYLLTSSDGITWTLYDSTSLLSTSTDTV
                     AWDYSAVETPSVVFFEGEYHLYYTAIAKSGIFAIGHATSLDGINWNKEDIILTPTNIF
                     DDWMSYLVGEPGAVVYHDKLYLYFTGVGNRFDAPAPAGKSVIGLIISDDGFNFGTPQQ
                     VLEQGEMYPPAENFYGYSTPFPIVLNDTLHLFFDVAQEDPEWLQVAIHHAYSVDGIND
                     FIQDEAAIFHKDDFDWTQREIRAPTVLADEGQLKMWFCGDDIFATAKWGIGYAWSNDF
                     SVNNIEFITEPITIFPNPAYDYLTIKSNVQWDNFTLTDLTGKIILSILSSTENNIDVS
                     FVPPGIYLAQLKSKEMILETRKIIISH"
     gene            10028..11161
                     /locus_tag="PLB46_00675"
     CDS             10028..11161
                     /locus_tag="PLB46_00675"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRF75052.1"
                     /translation="MKFNLRRITTSGNFIPEIDGLRFIAICRVVFCHLGGFIIEKVLN
                     RSSYAAGMGPFEHLLSNGRNGVEVFFAISGFILGIPFAKYYLAQGPKINLKQYFLRRL
                     TRLEPPYILVMTVLFFGSVFVIHDLTLSEGLKSYLASITYSHNFVYGSSFHPLINSPA
                     WSLEIEIQFYILAPLLALLFTIPKINKRILIFLSVALVFIFLNHSFDYSAYSIVNYLF
                     YFLAGLIIADIYISKVFILPKTRFDTVFATIAFCAIWLHTRLDFYVAWQKMIWELIQF
                     SSVFLLLYYVIIHGCFKWLKKKWVTNIGGMCYSIYLLHYPIISLVGNIIITKTVFGNV
                     YANIILYCVIILLAVLLISTIFYVLIERPCMRKDWYKRFVIFK"
     gene            11184..12548
                     /locus_tag="PLB46_00680"
     CDS             11184..12548
                     /locus_tag="PLB46_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75053.1"
                     /translation="MNKLLLSLSICYLSLSSLQAQLTDPAEIWDSIKNNTYVVVSPEF
                     EDILGTPYRQPLNSYGWEDGLQISADGLNLYTLYAPADLLSWFTYIASNPGLPICETI
                     GSTQFIRPYAETYGMDMAFNYFGCDTIMNLDILYAHRDNINDEFENWVLSDMARHGQI
                     EGGPFPLQSADNPNEVDHFLFTGPGDISMINNTNSNPDNIASAVLLPEPINSAAGEFI
                     VDNPVLRRINNSDTLLLVYEKYVDASLRDFMYAFSYDDGETWETPVTMTSINNATGHI
                     EHPQLHTDGSGTYLYYSRNFDIYRMKQSIPNNWDSWTNEQLLISKGNALAIGEPSIAI
                     NGDIAFVVAYHNIEHPEDEFDIDPWYVRNINGPNAIHHENNLPEIVVFPNPANANIQI
                     GNLLSSTNAVSIINMFGQEVAYVENYQTNATISVAHLPNGIYNVVLINPTGEKQSVQI
                     SVLN"
     gene            12573..13967
                     /locus_tag="PLB46_00685"
     CDS             12573..13967
                     /locus_tag="PLB46_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF75054.1"
                     /translation="MNKYILVVFLSTLFVSINAQNYTRAELITDINFLQTQLFEKHSG
                     LDTYTSLEEINNTLNQMKATLPDSMSAIQFYGHISPILSAIKDGHTMLLPPSVYLEKS
                     NTDSLFLPFRIFNSGDELFVELNYLPEDKIPNGSEIISINGFSAATILNTMLNGMMRD
                     GNNDGYARWALNWWFMEYFSYYYNHPSSFEIQYINANGSIQQTIINASSKEEIFENRT
                     KRYPNRSFTRTYDQKPGTAVVFTLDTIHSIAIIKIKDWDEDILKNTYHQKIRKTIDSC
                     FDSIFLCNIQHLIIDIRDNQGGNMINSKHVLSYLLDTTFNLVDNYYKVSNPALDTGNS
                     RYKKTSGEKSKFNKIRKTIFTGKVYVLTNGGSFSNSGLFASTLKRNNRALFIGEETGG
                     SIYSLNANTKSLTLPNTQIQFELTTKRFAINESIPNAGRGLIPDYVVHPTITDLVEGR
                     DVILEKVLDVIQGN"
     gene            14106..15710
                     /locus_tag="PLB46_00690"
     CDS             14106..15710
                     /locus_tag="PLB46_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75055.1"
                     /translation="MKFSVALFSLLLFHIFNTTAQSPFEYAVKIDSIQITGLPGLHSY
                     AFAEHDGKWLIIGGRTDGLHPRQPFASFPEADNNTDVYVIDIENKQFWSSSLNVLPTA
                     IKEQLQSSNMNFYQDADTLVLIGGYCYAQSAATHKTFPNLTTVNVSGLINAIITNADI
                     NSFFKQITDDKFAVTGGELAKLGNQFYLVGGQRFDGQYNPMGHGTYVQTYTEAIQKFT
                     LNNSGATLSYSNYEKITDPIHLHRRDYNLLSHIFPDGSEGLVISSGVFQQFVDLPFLY
                     PVEIKEDGYVAKTEFNQYLNNYHTAHTNIYDSLENKMSSLFFGGISQYYYVDGALHQD
                     DFVPFVNTISGISRDNSGNLTEFYIPTGMPGYKGSSAEFLPNYNLTYNHSEILRETQF
                     EGDTITIGYILGGISSPSKNPFSMNQTSLTSADNNIYIVKLYRAEPNSIKPIHVQNPY
                     TMELFPNPASTSFTIKFNVNNIVNARYYISNMSGALLQSNPIEITQAGEVKYLVTLDK
                     SYLLQNLIVTLVIDNTYYLSKSLVLE"
     gene            15745..16128
                     /locus_tag="PLB46_00695"
     CDS             15745..16128
                     /locus_tag="PLB46_00695"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF75056.1"
                     /translation="MPKSKKPKLSDEEQVAEYMLQLAHPLKAEIELLRSIIKNANNNL
                     KERIKWNAPSYYTNVDLLTFNVKSDNTILLILHHVTCVKLKSDLLEGDFPDRRIIYFK
                     DADEINSSKKELERIINEYVALAAA"
     gene            16188..18617
                     /locus_tag="PLB46_00700"
     CDS             16188..18617
                     /locus_tag="PLB46_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75057.1"
                     /translation="MSFKHRLLLLLIVCLPLVKAYSQGGCLEYNNRLFVINIVNNGLP
                     VKDSQIKVYLVNEDSLPYWYETNSVDNYNLKLTPRKIKRNNPKNAKNQKNPYETQTYY
                     PKLNSNWYTCKIREFSPDLPEQLYQVLVINKKTKDSVYTYLPYSKSIDICKNNLDREI
                     PGDLFFDDGSNFEPITIDIAVRKVFPLPQYPITNTYLKFDYTKKWNAERGDTLLYVYQ
                     ARVHDKNTLAVIQKIPIENALPFPSNYFKQNIETGDFYKNNPKGVKDFRIMTAQVLND
                     RNQVLSSSFDHYVFDADQQKYVYDKILSTSNNVAIKENTPVITKCEISVTAKEKYYDY
                     FKLVDGEWVFTERKTEYLFEANPFKYQKDAIIWPEKSNGFLPVVQMPYDDKYYTHFDS
                     FALLNNSRDTLLLSLSHSYSKNTFSIPEKIAPKETVYLKYSEWLQPSPGYVYLIERNT
                     YVYFGDKSSEIKGFNYFLAAPGTEERDKQTGKIVRYNSKIDTVSNTGMVLEVYDTGMP
                     KAFGLFNYNNGKKVDRWENWDEKGNRLQAAEYGKKLTALIGNPDRLQDEVSIRVKTKT
                     EWKNMEFKRNENTFILFVPMDADSLEIISGEFRVDSKFTQWNSTSQANFTYYLLNSND
                     DYIDQMGIRYPITWLENEYLINWDISFYSYSKPGTIENEVKRLQAKYPGVSFMSFVYA
                     PHLIQVFINPTTESKKQQLIQNLINEASIGSLSQGASMLNSGKPMYLETSATIIVSPY
                     IRQELVQEIADKYNGKLSQMFGTGNYYQLDLSSTMYDKSYINLLHTIQKEPNVIGISA
                     GWRTDINYLDE"
     gene            19151..19339
                     /locus_tag="PLB46_00705"
     CDS             19151..19339
                     /locus_tag="PLB46_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRF75058.1"
                     /translation="MPSGMEWIIILFAVLLLFGGKKIPELMRGVGKGIREFNDAKNNV
                     SREIRDGMNDEPKKSEEK"
     gene            19781..21409
                     /locus_tag="PLB46_00710"
     CDS             19781..21409
                     /locus_tag="PLB46_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRF75059.1"
                     /translation="MHYIRITVLCLLLWGVAKAQVSNSTVSTNNGISLNATDESVNVM
                     DSLLEAYFSTNPAWNFDQRDYDMLGYAATDTPRFTPEIYAERISKINSPIPYTYNKSV
                     QGFLDLYCMRRRTQVSNMLGLSQTYFPMFEAELDRKNMPIELKYLAIIESALNTHAVS
                     KAGATGLWQFMNGTGKLMGLRIDSYVDERRDPYMATVAAVAYLEDLYNIYNDWFLAIA
                     AYNCGPGNVNKALRKAGGGDKTFWDIENYLPKETRGYVPAFIAATYVFEYHKEHNIRP
                     AKYDYDFSMMDTLMITHKMTIEQLAPYVGLTAEEIALNNPALKTKTIPGSPYPYPLRL
                     PMNAVATFYANKDSLYASLNKKETQNLATLAKNVEEVNNAKAKTATKTTTDATTAATT
                     TTTKEITDPEAPVTVSYTVKKGDNLGYISDWFDCSVADIKKWNKLSSTKIVPGQKLKL
                     TVPAKHEEQYAMINKMTSAEKQKLTDIQLISAAPAEKEPATKEVIYYTVKPGDTLWGI
                     SQKYPDNTVDKIRELNGLEKNETLKTGTKIKLVK"
     gene            21633..23381
                     /locus_tag="PLB46_00715"
     CDS             21633..23381
                     /locus_tag="PLB46_00715"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF75060.1"
                     /translation="MQRRFLFVFLCMIFLSSSQLLNGQNTIQFKKETIDSVLETFDHS
                     TRLPGYALAVVHKGEVIYTAQRGKASIDDNIKITQSTQFNIGSVAKQFTGAAILLLEE
                     AGKLSLTDDIHKYIPELPDYGHTITLQHLLNHTSGIRDHVELATLISEDKDELNTFPA
                     MLEWQKKYPALNGAPGTSFAYSHTGYMMLAMVIERVSGLTYAQFLQNNIFTPLNMMNT
                     YVEEGKNNRLRDGSTHYELNHTNTKAKKAEAYTNPLGASGIISTTEDLAKWDKNFLQN
                     KLGKGGNGLISKMEKSGSLNDGTDVHYGAGLLIKSYRLKPIAEHSGGQGEYLTLYRRF
                     LNEDLSIIVCMNSYLISPFDICDAIANNIMTYKEQHWLVQKSLPDTTFKKLEGTYAAE
                     NNLVRYVYRTKGQLVIARIFDEDTTAYQLTYTGTTSSGDYAFADTLGSTVIFNVEGDK
                     IAGLNWDGGTYFICNRYYTKENTTVPPKLTSFAGKYYCAALDKKVRIQYFSKKDELTI
                     HPFPFISYNLISKGGGIYVVEDQPYIVNFNKNEFMIGNDWIFNIKYDKKIKPKKEKKE
                     KPEKEKKGLFGWFGKD"
     gene            23391..>24436
                     /locus_tag="PLB46_00720"
     CDS             23391..>24436
                     /locus_tag="PLB46_00720"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF75061.1"
                     /translation="MKFSFFVIGLICWFDTFAQFETDITYRQLDSIIYGKNPDTEAPG
                     MGAIVIKDDSIVYFTSTGYANTKRKLPIGLHTQYNIGSITKMFTATAILLLEEEGKLR
                     RTDEIHQYIPELPEYPFPITINHLLGHTSGIRDHFEVARFLFNYNNKLVSFEGMLDYQ
                     NEFPELNTNTGESFAYSNTGYMLLAMIIEKVSNQSYESYLAENIFQPLGMENTFATRE
                     KQKYLSDGTANYPQKKNGKFKGPLDYYDALGATGVYATLYDIYLWDKNFYHNKLGKLS
                     QSLIDTLQHSFALNSGESVNYGCGIIEKKYRGKVAYEHSGGWGYYLTQYRRFPNEHIS
                     VIAWNNSDNYSPFTT"
BASE COUNT         8237 a         4370 c         4335 g         7494 t
ORIGIN      
        1 attatctact ggatatataa attacgtttg gaacgtgcac ttgccgttga aaaaatgcga
       61 accaaaattt ctcgcgattt acacgacgat ttgggttcca ctttaagcag tatatctatt
      121 ttgagcggac aggtaaaatc tcgactcatt aaacaacctg aaacggttga gcccatttta
      181 gataaaataa acgaaagtgc tattaaaatg aatgatagtt tgcaggattt agtatgggcg
      241 gttaaacccg aaaacgacac tttgcaggaa ctgctggttc ggatggaaca atttgcctca
      301 ggaattttgg aatctaaaaa catcaattac acttttaccg gaccgacaac tgtatctgat
      361 attaaactta atccggagct tatccgtaat atttacctca tttttaaaga agctgttaat
      421 aatgcggcaa agtatagtaa ttgtgcaaac gttaccatca cagtaaaaga aaaaggtaaa
      481 caattgagtt tgtcggttat tgacgatggt attggattta atcctgaaac cgtgaaaaga
      541 ggcaatggat taaacaacat gacaaaacga gcagcgcaaa tcgggggttc gttgaaaatt
      601 atatctgagc ccaacaaaca aagcggtatt gtgctggaat gtaaaatcac atcatgatgt
      661 gataatttag cactatccaa atacacaact ttacacctta tgaaagtgtc gtttttaatg
      721 tttgaagaca ataatgaact tcgcgaagca ctagttacag cgcttaacga acgcaatgag
      781 gtggtgtgta ttggtaatta cgacaatgct attcatgcca cacaacatgt aaaggaacta
      841 aagcccgatg ttattctaat ggacatcgat atgccgggca taaatgggat agaagcagtt
      901 aaacaaatta aatcgcagca tgtaaatgtc gaaataatta tgtttaccgt atttgatgaa
      961 aacgataaag tgtttgacgc tatttgtgcc ggagcaaccg gatatttatt aaaaaacagt
     1021 agtactgaaa aaattgcaga atctattgtt gatgttgcca atggaggagc gccaatgagt
     1081 gcaacaatag ccagaaaaat attatttcag tttcctcagt ttaaaaccgc cggcaaagaa
     1141 aaatttaatc taagcaaccg tgaagtggaa atattaaatt atcttgttaa aggaaaatca
     1201 tacaaaacaa ttgcacaatt gtgttttatt tctcacgata ccgttcgcac acatatcaaa
     1261 cacatttacg aaaaactcca agtacattcc gctcccgaag ctgttgcaat tgcaatggag
     1321 cacaggcttg ttaattaata gccgataaat atcacatcac aatgtgattg ccctgtttat
     1381 tgcgcaaatc tacttttgca ataaacaatt aattatgaaa aaattatggt attttattat
     1441 tacttggatt ttaatatcca ttcccgtgtt catttttgca caaccacaac cacaacacaa
     1501 taactctgct attacccaaa caacaaacac aaatgtttta aatgaagtga taattattgt
     1561 caaataacaa taacctaatt caaccaactg tcatgaaaaa aataatcata cttatttcac
     1621 tttattgtag tgtattaata accactgcac aaaatgttgc cttaaacata gatggttctg
     1681 cgccggacaa ttcttcgatg ctggatatta agtccactac caaaggttta ttaattccgc
     1741 gcatgacaca agcacaacga actgctatag cctctcctgc aacaggcttg ctgttatttc
     1801 aaaccgattt aaccaaaggg ttttattatt atgatggtgc ggcatgggtg tttattggca
     1861 acagcacagc cggatggtct acaacaggta attccggaac aacaccgggc acaaactttt
     1921 taggcacaac cgatgctaaa gatttaaaaa taaaagtgaa taacacagtt gccggttcaa
     1981 ttgaaacatc cggaaatatt tttttaggat taaatgccgg catcgtaaat accggtgcaa
     2041 ataataccgc acttggtgga aatgcattaa aagctaatac aacgggtgaa caaaataccg
     2101 taattggaac atttgcaggc gaattaaata caaccggctc ctttaatgta attattggtg
     2161 aagaggcttt acaaaataat tctactactt ccggcaatgt tgccatcggt tataaatcgt
     2221 tggaagcaaa tgcaacttat ggcgataata cggcagtggg ttatcaatcg ttaatgtcca
     2281 ataatgccgg atattataat accgcctttg gtaaatcaac gcttgaagat aatacctcag
     2341 gatttttaaa tgttgcgcta ggtgttgatg cattggccaa taatactagt ggtggttgga
     2401 atgcagccgg tggtgcatat gcattatacg ctaacacaac tggaagcgca aatacaggat
     2461 ttggttatgg tgcaatatct acaaatacca ctggttccaa caattcagga ttaggtaata
     2521 tggcactatc tttcaattca acaggattta gtaatagtgc tgttggtagt tatgcattat
     2581 acgcaaatac tacaggcgct gataacactg cagttggtac ttcaagtatg aatcaaaacg
     2641 cagttggaag taataatact gctgttggaa gtaaagcctt atattataat accgcaaata
     2701 ataatactgc agttggaaca gagtcactgg agggcaacac aaccggaact gcgaatgctg
     2761 cattgggtta tagagcaatg gaagataata ctacaggttc aaacaataca tcagttggcg
     2821 ataatgcatt agcaaataat acaacagcaa gtaataatac tgcaatgggc tcaggtgcat
     2881 taaaattaaa tacaacaggt ataaaaaata cggctattgg tacttccact ttaactgcca
     2941 atattaccgg aagcgaaaat actgccatcg gttataatgc cctggcagca aataccacaa
     3001 gcacaggtaa tgttgcagtt ggtgcaaatg cattacaaac agctactaca ggaagtagta
     3061 atactgcagt tggatataat gcactcatga acgctaccat tgctacacaa aataccgcaa
     3121 ttggatatca aaacctgagt gcattaacca gtggctcaca aaatatagca gtaggtagta
     3181 atattttaac ggcacttact gtttcagcaa ataacattgg tattggacat aacgtattaa
     3241 cctcaaatgt atccggcgga ttaaacattg gaattggttt taatatatta accagtagca
     3301 cttcttcaaa taatattggt attggaaata atgcactcgc tgcaagcaca accgggcaac
     3361 aaaatatagc aattggtatg ggtgcacttg atcaattaac aatttcttca agtaatatcg
     3421 gaatcggcac aaatgcatta ggggcaagta ctaccggtac cgggaatacg gctataggag
     3481 gctccacaat gtttaataat acaacaggtg gaagtaacgt tgcaattggt aacagtgcat
     3541 tattgaccaa cactaccggt agtagcttga catgtgttgg agaaaatgca aatactggcg
     3601 gagctgcact cggaaattct tctgccattg gcgcacgcgc acaagttaac accagcaata
     3661 gtatggtgct tggtagcgta aatggtgtaa acggcgccgt tagtaatgta ttcgtaggaa
     3721 ttgccacgaa ctcacctgca catattttag aattaggtgt aaacgatgct gcaaaaccaa
     3781 cttcttcaac atggacagtt gtatcagatg agcgattaaa ggaaaatatt aaagatttta
     3841 atgatggact cgacctggtt atgcaaatta atcctatctg gtttacttat aacggagaag
     3901 caggattacc acatgaaaca ggtgttggaa caattgctca ggaattacaa aaaattgcac
     3961 cttacatgat taccaaatgg acttaccgcg aaagtgatac cgatcagggt atggaatatt
     4021 tgggtgtaga ctatggtgct atggattttg ttttggtaaa tgctattcag gaattaaata
     4081 atcaggtaaa acaaaattcc tctgccgaag aagtagctta taacaaacaa aataatgcaa
     4141 ttgaacaact ccaaaatgaa aatgcagcat taaagaaaca attaactgaa attgaaaaac
     4201 aattaaatgt tctttcagat aaaatgcagg caagtgttgc taaaaaataa atgtatggta
     4261 aataagggat tatacggata tagtttatta ctttttgtat tttctacaat aagtagttac
     4321 gggcaagcag gactacgtct tcaaaccggt gcgcaattga ttataaacgg cagtcccgaa
     4381 atagtattaa acaattgcgg gttatataac gatggcgata ttgatgccgg acttggtgct
     4441 tttagaataa caggtacagc ggcaacatcc aattctgtta ttgcaggcag cagttatact
     4501 actttttacg atttaacact gcaaaaatta tctaatgaca tgcagatgga gcagcattgc
     4561 gctattacac attttttaac ctgtgatggt ggtttattag aattaaatgg atatgtgatt
     4621 gaccttggca caaccggtat aattaaagta gaatctgaag atacacgtgt aaccggaaca
     4681 tcaggcggag aaataattgc tactgcaact ctaaatgcac cttctggggc aaaccccggt
     4741 aatttaggcg cttctataac cagtacagca aacttgggtc ttacaacagt tcgtcgcggc
     4801 catgtgcaac aaactgaccc atccggaaat tatagtattt atcggtatta tgatataagt
     4861 ccaaccaata atgcaggatt aaatgcactt ttaattcaat attattttga tgccgaaaat
     4921 gcaggccttg atgaaaataa ttttgaccat tatttatccg acaatgcggg agcaacatgg
     4981 aataacctcg gacaagatgc cagagaccct acagataatt ttgtaaaact ttccggttat
     5041 gatgcatttg ccagacaaac gcttgccgac ccgattggtt ctcctttgcc tgtagtatta
     5101 attgattttt atgcgcagtg tacacttaca agtactgtat taaattggtc cacagcgtcc
     5161 gaaattaaca gcgaccattt tgaaattcaa caattagatt tcaatcaaaa ttgggttaca
     5221 ataagtacta taccggctca gggatatgct tcacaaacta cgaattacac ctatagttta
     5281 cccactagta atacttttta cagattagta ttggttagta caaatggtga tgccgaaata
     5341 ttagaagtta tacaaagcga ttgttacatt gaaccggcaa ccgcaattta tccgaatcca
     5401 actactgatt atttacatat tgattttggc aatcacgaca atgctcaggg acaaatttta
     5461 atttatgaca tgcatagtca actggtacag caattactac ttcaggaaaa ttccggtaaa
     5521 atacaaattg atgtgcgaat gttcccgaca ggaatatatt tgttggaaat acaggaaggt
     5581 aaaaacaata aacattttct ttttaataaa caggataaac gttaatggtg aattaattag
     5641 ctgtttagct tgaaggttgt attaggtatc aaatttggca ataattgcta aagttggtat
     5701 atttgataaa caccacacgc catgcaacga cttatagata ccattcaaaa gctggaaccc
     5761 ttaactaacg agcaattgaa ggccattgaa caaatgcgga gcattgcgca ttataaatct
     5821 ttgaaaaagg gtgatttcat ttttacaagg ggtgatacac cacccatttc cgttttcgta
     5881 gccgagggta tgttaaggca ttttgtgagc gataataatg ggaatgaaaa aattatccag
     5941 ttttttatgg aaggtgcatt ttttgatgat tgtggtagtt atgtcaataa tcagccaatt
     6001 gattattata ttcaggcaat agaagatagc gaaatcattt ttttctacct tgacgagctt
     6061 aaacaattag ccgcagactc accattaatt gaaaaaattg gcactaaaat cgcggctgat
     6121 tttttaaaaa accatcagga acatcttacc attttaatga aatataatcc ggaagaacgg
     6181 tataaatatc tcatcacaaa caaacctgaa tttgttcaaa gggtttctgt aacacattta
     6241 gcccaatttt tggatgtttc ccgcgaaacc ttaagtcgta tgcgttcccg ttttgccgaa
     6301 caaagcattt tgtgatgcta atcacacccc ataataacag ccaattctat ttttgccgct
     6361 aaacattcgc gcaatggcta ttgatatata ccaaaaactt gtgttaggca aagaaacctt
     6421 tcttccaata aaatccgatt ccgatacttt tcaaatggac tggtggatgg agccgggagg
     6481 tgttgttcct cagcatatac atcgttatat ggatgaacat tttgaaatta tctcaggaac
     6541 ggcaatattt atagttgacg ggaaaaaaat taccaaaaat aaaaatgatg aattatttat
     6601 acctaagggt gttgcgcatg gtataaaaaa taacagtagt gagattttaa atataaaggt
     6661 aacctatact cccgcagcag atacagcaca catgtttaaa ataatggctt tcctcaacca
     6721 aaaatatccg ggtcaggcat ccaacatggt gaaatatttt tatatgtttc caaagcttgg
     6781 attaaaaccc ttttctgaaa ttccttcacc atttattatg cgcacactag ccgcaatttt
     6841 aaacatggtg gggtttttgg ctggttggaa caagctggtt aaagcctata agcaaacaaa
     6901 ctgattgcta taaactactc ttattgggat ttttcattaa tgtggagtca cccacctgtg
     6961 aatgttaaac tattcatatg agaagcattg cgttcacatt atgacaaaga atcgtatcgt
     7021 aaaggcgtta tatggaaatt acaatgggat gaatacaacc aaataaatct taggatgcat
     7081 taggcttaca aagttgaatt tctattattt tgtctttatt ttaggtaagt tcaacttcat
     7141 tattgtcata ttaataagca ttttacatct aaaaaaataa tttgctattt ttatgtaaac
     7201 cttcacctac atgaaaaagc tgtttttgct cctatttacc agtttattta ttacggatat
     7261 ttatgcccaa accaatattt accatgctct tcctgatagt gggtactact ggaatgtaat
     7321 cgatcttaga actgaatgtg aaactggttc ggccgacact attaaaggtt tatttaagta
     7381 tgctacaatc cagggagata ctttagtagg cgccaaaagg tataaaaaag ttcatgctac
     7441 aaacagtttg aaagaaattg tatattttct gggttactat tatgaggata cagtaaatag
     7501 aaaagtttac gtagtcacag acaaccccga tgaccatacc attaccgaag aatcattatt
     7561 atacgatttt aatataggaa ttggtgaaac agtggatgat tattacgatt ttcggggatt
     7621 cgatgaagat catcccgcta attcaattgt aatttcagtt gattcaattg aaatcgaaag
     7681 tgtttataga cgaagaattg gaattcaata tgaactgttt caatgcgata acaaaaagga
     7741 aattaaaata gatacaacct attggattga aggaattggt aacaccgccg gctttgcgct
     7801 cacaaaccaa cttccgatga atgcaggaga ttacgcggtg gatgttttag catgtgtttc
     7861 taatggtgcc gaagttgttt ttattaatcc acatgaatgg cataactgcg attcggtatt
     7921 tctaaaattt ataccaatta tcgatttaaa tagcgaggaa atagttaccg tctatcccca
     7981 accggcagat caacttctta atattgaatt aaacttaggc tcaagagaag gtaattttac
     8041 aattcaaatt ttggatttat cagggaaact tgtctaccgg caagtgtatt ttactgctga
     8101 tataaatatt ccggtaaata atattccaac tggaatttat ttaataaaaa tacaatatga
     8161 aaccggtgtt tatttttcac aaaaaataat tattcagcac tagcatattc aattttctaa
     8221 tataaaattt accggtatga aaacattagt tttatttttt tgcatttccg tgactctgta
     8281 ggcttaagat tcattcctat tactttgttc aggattttct taaaaaaatt gtaagtttga
     8341 gcatggctta taatgaaaaa ctggctaatc gcatacgtga gcggtttgct gaacttcacc
     8401 ctgtagaaga aaaagaaatg atgggcggac taacctttat gtacaatggt aaaatgtgtg
     8461 tcggcgtaat taaagatgac cttatgtgtc gcattgatcc taaaatacat aacgaatgtg
     8521 ttgaaaaaca gggatgtaaa actatggatt ttacttcaag accaatgatt ggttatgtta
     8581 taattgatga aaccggcatg aaaagtaaaa aggattttga ctattggata aatcttgctt
     8641 tagcgtttaa cccatatgca aagtcttcga aaaaaaagaa tagcaacaaa aaataacttt
     8701 aatccctgca ttatgcaatt acttaaaaat tatgctataa ctattctcct gctgttaagt
     8761 gtaatcggca caaatgcaca atcctttaca cctcatcccg ataatcctat attacccctt
     8821 tcttttgtta accccggaac atggaacgac ccatgtgtat taaaacaagg tgatacctat
     8881 tggatgtatg cttcatctaa aaatggtgtt gatatttttg atggtgaggt tgttccttat
     8941 ctgctgactt cttcagatgg tattacttgg acattgtatg acagcacatc gttactcagc
     9001 acctctaccg ataccgttgc atgggattat tcggcagtag aaacaccaag cgtagtgttt
     9061 tttgaagggg aataccactt atattatact gccatagcta aaagcggcat atttgccatt
     9121 gggcatgcca catctttaga tggtattaat tggaacaaag aggatattat tttaacccca
     9181 acgaatattt tcgacgattg gatgagttat ttagtcggag aacccggcgc tgtggtttat
     9241 cacgataaac tatatctcta cttcacagga gttggcaatc ggtttgatgc tccggctcct
     9301 gcaggtaaat cggtaatcgg attaattatt tctgatgatg gatttaattt tggaacgcct
     9361 caacaagttt tagaacaagg ggaaatgtat ccacctgcag aaaattttta tggctattca
     9421 acaccttttc caatagtact gaatgatacg ttacatttat tttttgatgt ggcacaagaa
     9481 gacccggaat ggttacaggt agcaattcat catgcctatt cggttgacgg tatcaatgat
     9541 tttatccaag atgaagctgc tatttttcac aaggatgatt tcgactggac ccaacgtgaa
     9601 ataagggctc caacggttct agcagatgaa ggtcaactca aaatgtggtt ctgtggtgat
     9661 gatatttttg ccactgctaa atggggaatt ggatacgcat ggtcaaatga tttttcagtc
     9721 aataatattg aatttataac cgaaccgatt accattttcc caaatccggc atatgattac
     9781 ctaactatca aaagcaatgt gcaatgggat aattttactt tgactgattt aactgggaaa
     9841 attattttat caatattatc cagtacggaa aataatattg atgtttcctt tgtaccacct
     9901 ggtatttatt tagcacaatt aaaatcaaaa gaaatgattt tggaaacgcg caaaataata
     9961 atatcacatt aaacgctttt gtagattatc tcttttctaa aataaagatt aacattgtgg
    10021 tattatcatg aaatttaatc tccgtcgaat caccacctcg ggtaacttta tccctgaaat
    10081 cgatggattg cgatttatag ctatttgcag agtagtgttt tgtcatttag gcggttttat
    10141 tattgaaaaa gtgctcaacc gcagctccta tgccgccgga atggggcctt ttgaacatct
    10201 gttatccaat ggaagaaatg gcgtggaagt gttttttgcc atcagtggtt ttatcctggg
    10261 aataccattt gccaaatatt atctggcgca gggaccaaaa attaatttaa aacaatattt
    10321 tctcagacga cttaccagac tagaaccacc gtatatcctg gtaatgaccg tgctgttttt
    10381 tgggagtgta tttgttattc acgacttaac attatcagaa ggtttaaaaa gttatctcgc
    10441 ttctattacc tattcacata attttgttta cggaagtagt tttcatccct taattaacag
    10501 tcccgcctgg agtctagaaa tagaaattca attttatatc ctcgcaccat tattggcatt
    10561 gttatttact attcctaaaa tcaataaacg gatattaatt tttttaagtg tagcgctagt
    10621 attcattttt ttaaaccaca gtttcgacta tagtgcttat tccatagtga attatttgtt
    10681 ttacttttta gctggattaa ttattgccga tatttatatt tcgaaagtat ttattctacc
    10741 taaaacacgc ttcgatactg tatttgcgac tattgcattt tgtgctatat ggttgcatac
    10801 tcgcttggat ttttatgttg cctggcaaaa aatgatatgg gaattaattc aattcagcag
    10861 cgttttttta ttgttgtatt atgtaattat acacggatgt ttcaaatggt tgaaaaaaaa
    10921 atgggtaaca aatattggtg gaatgtgtta tagtatttac ttattgcatt accccattat
    10981 aagtcttgtg ggcaatatta ttatcaccaa aacagttttt ggtaatgtat atgctaatat
    11041 tattttgtat tgtgtaatta tcctgttagc cgttctgctc atatccacta ttttttatgt
    11101 gttaattgaa cgtccgtgca tgcgaaaaga ctggtataaa cgatttgtta tatttaaata
    11161 acaattttgt ttatttttaa tgtatgaaca aattattact cagtctgagc atctgctacc
    11221 tctctctttc ttcccttcag gcacaactaa ccgatcctgc agaaatttgg gatagtatta
    11281 aaaacaatac ttatgttgta gtgtcccctg aatttgaaga tattttaggc accccatatc
    11341 gccagccact taattcctat ggctgggaag acggtttgca gatttcagct gatggtttaa
    11401 acctttatac cttatacgcg ccagccgatt tactttcctg gtttacttat attgcttcaa
    11461 accccggttt accgatttgt gaaacgatag gaagcactca atttattcgc ccttatgcag
    11521 aaacgtatgg tatggatatg gcatttaatt attttggttg tgatacgatt atgaatttgg
    11581 atattttgta tgctcatcgc gataatatta atgatgaatt cgaaaactgg gtattatctg
    11641 atatggcaag acacggacaa attgaaggtg ggccatttcc attacaatcg gctgataatc
    11701 ctaatgaagt tgaccatttt ttatttaccg gacccggtga tatctcgatg ataaacaaca
    11761 ccaattcaaa ccctgataat attgcatcag ctgttctttt accggagcca attaattctg
    11821 ctgcaggtga atttatagtt gataatcccg tattaagaag aataaataat tccgatacct
    11881 tattattggt gtatgaaaaa tatgttgatg ccagtttaag agattttatg tatgcattta
    11941 gttacgatga tggcgaaacc tgggaaacac ctgttacaat gaccagcatt aacaatgcta
    12001 ccggccacat tgaacatcca caattacata ccgatggcag tggaacctat ttatattatt
    12061 cccgcaattt tgatatttat cgtatgaaac aatctattcc caataattgg gatagttgga
    12121 caaatgaaca gttattaatt tcaaaaggta atgcacttgc aataggtgaa ccatctattg
    12181 ccattaatgg tgatattgca tttgttgtgg cctatcataa tatcgaacat ccggaagatg
    12241 agtttgatat tgatccatgg tatgtgcgaa atatcaatgg cccaaatgct attcatcatg
    12301 aaaataattt accggaaatt gttgtatttc ctaatccggc aaatgcaaat attcaaatcg
    12361 gcaatttgtt gtcttcgaca aacgcagtaa gtataataaa catgtttggc caggaagtag
    12421 catatgttga aaattatcag actaacgcca ctatttcagt tgcacatctt cccaatggaa
    12481 tatataatgt agttttaata aatccaacag gtgaaaaaca atcagtgcaa atatcagtat
    12541 taaattaaca caagtaattt aatctatcaa cgatgaataa atacatacta gttgtatttt
    12601 taagtacttt atttgtatca attaatgccc aaaattacac gcgcgctgaa cttatcactg
    12661 atatcaattt cctccaaacc caacttttcg aaaaacattc tggattagat acctacacat
    12721 cacttgaaga aataaataat accctcaatc aaatgaaagc aacattgcct gattcaatgt
    12781 ctgcaattca attttatggt catatttcac ctatacttag cgcaataaaa gatgggcaca
    12841 ccatgttatt accgccttca gtatatctcg aaaaatcaaa taccgattcc ttgtttttgc
    12901 cttttcggat attcaattct ggtgatgaat tatttgttga actcaattat ttacctgaag
    12961 ataaaattcc gaatggctct gaaattatta gtatcaatgg tttttcggca gctactattt
    13021 taaacaccat gctcaatggc atgatgcgcg atggcaataa tgatggatat gctcgctggg
    13081 ctttaaactg gtggtttatg gaatatttta gttattacta taaccatcca tcttcttttg
    13141 aaatacaata cattaacgcc aatggtagta ttcaacaaac catcattaat gcaagctcaa
    13201 aagaggagat atttgaaaac agaaccaaac gttaccccaa tcgttctttc acacgcacat
    13261 acgaccaaaa accaggaaca gctgttgtat ttacactcga tactattcat agtattgcca
    13321 ttataaaaat taaagactgg gatgaagaca ttttaaaaaa tacctatcac caaaaaatca
    13381 gaaaaacaat tgatagttgc tttgacagta tttttttatg caatatccaa catttgataa
    13441 ttgatattag agataatcag ggtggtaata tgattaacag taaacatgta ttatcctatc
    13501 tactggatac cactttcaat ttagttgata attattataa agtatccaac cctgcacttg
    13561 atacaggaaa ttccagatac aaaaaaacaa gtggcgaaaa atcgaaattc aataaaattc
    13621 gaaaaactat ttttacagga aaagtttatg tgcttaccaa tggtggtagc ttttccaact
    13681 ccggactttt tgcttcaaca ctaaaaagaa ataatcgtgc cttgtttata ggagaagaaa
    13741 caggtggcag tatttatagt ttaaatgcca atactaaatc acttacactg ccaaatacac
    13801 aaatccaatt tgaacttacc accaaacgtt ttgctattaa cgaatctatt ccaaatgcag
    13861 gcagggggct tattccggac tatgttgttc atccaacaat aacagatttg gtagaaggaa
    13921 gagatgtaat tttggaaaag gttttggatg taattcaagg taattaatgc ttattttttt
    13981 gattttgtca ttgctcaatt tccaatgtat gtcaccaatt aactgttgca atttataatg
    14041 tagttaagtg acaaaagtaa catttaccct ttcgaattaa acgtaacttt gcacaaagtt
    14101 ttgttatgaa atttagcgtt gcccttttta gcttattgct attccatatt tttaatacta
    14161 cagcacaatc accatttgaa tatgctgtta aaatagattc catccaaata acaggattac
    14221 ccggcttaca ttcctatgcc tttgccgaac acgatggcaa atggttaata ataggtggca
    14281 gaaccgatgg tttacatccg cgacaaccat ttgcatcttt tccagaagca gataacaata
    14341 ccgatgttta tgtaatcgat attgaaaaca aacaattttg gtcctcatca ttaaatgtat
    14401 taccaacagc aattaaagag cagctccaat cgagtaatat gaatttttat caggatgccg
    14461 acaccctggt tttaattggt ggatattgtt acgctcaaag cgccgctacc cataaaacat
    14521 ttccaaactt gacaaccgta aatgtatccg gcctaataaa tgccattata accaatgccg
    14581 atattaactc cttttttaag caaataactg acgataaatt tgctgttaca ggaggcgagt
    14641 tagcaaaact tggcaaccaa ttttatttag tcggtggaca acgatttgat ggccaatata
    14701 atccaatggg tcacggcacg tatgttcaaa catatacaga agccattcag aagtttacat
    14761 tgaataatag tggtgcaact ttatcgtatt ccaattatga aaaaattaca gacccaattc
    14821 atttacatcg cagagattat aatttattat cccatatttt ccctgatggg tcagaagggc
    14881 tggtaatttc atccggtgta tttcagcaat tcgtcgattt gccattttta tatcctgtag
    14941 aaattaaaga agatggttat gttgcaaaaa ctgaatttaa tcagtactta aataattatc
    15001 atactgcaca tactaatata tatgatagtt tggaaaacaa aatgtcatcc ttattttttg
    15061 gcggaattag tcaatattat tatgttgatg gtgcattaca tcaggatgat tttgtgcctt
    15121 ttgtaaatac gataagcggc atttcccgcg acaattccgg taatctaacc gaattttaca
    15181 tcccaaccgg aatgccggga tataaaggaa gcagtgccga atttttacct aattataatt
    15241 taacgtataa ccattcagaa attttaagag aaactcaatt tgagggtgat accattacta
    15301 ttggttatat tttaggtggg attagcagtc cgtcaaaaaa cccgtttagt atgaatcaaa
    15361 ccagcctgac atcggcagac aataatattt atattgtaaa attgtatcgt gccgaaccca
    15421 attccattaa accgattcat gtccaaaatc cctatacaat ggaattattc cccaatccgg
    15481 catccacatc atttaccatt aagtttaatg taaacaatat tgtaaatgcc cgttattata
    15541 tttccaatat gtctggtgca cttttacagt ctaatccgat agaaattact caagcaggtg
    15601 aagttaaata tctggttaca ttggataaat cctatctttt acaaaacttg attgtcaccc
    15661 tggttattga taacacctat tatttatcga aatcattggt attggaataa atggggttcg
    15721 cttaaatttg attaccttta cacaatgcca aaatcgaaaa aaccaaagtt aagcgacgaa
    15781 gaacaggtag ccgaatatat gctgcaatta gcacacccgc taaaagcaga aattgagtta
    15841 ttgcgcagta tcataaaaaa tgcgaataac aatttgaaag aacgtattaa atggaatgct
    15901 cccagttatt acaccaatgt tgatttgctc acatttaatg taaaatcaga caataccatt
    15961 ttactcattt tgcaccatgt tacctgcgta aaattgaaat ctgatttatt ggaaggcgat
    16021 tttcctgatc gcaggattat ctattttaaa gatgccgatg aaattaactc cagtaaaaaa
    16081 gaactcgagc gtattatcaa tgaatatgtt gcacttgcag ccgcataatt agcatcattt
    16141 tatggaaatt gcttatgtgt tgcatcctaa tagaaagcac cacaattatg tcttttaaac
    16201 accgactcct cttactgctg attgtttgtt taccgcttgt gaaggcctat agccagggcg
    16261 gttgcttgga atataacaac cgtttatttg ttattaatat cgtaaacaat ggtttaccgg
    16321 ttaaagacag tcagattaag gtatatctgg tgaatgaaga tagtttacct tattggtatg
    16381 aaacaaattc cgttgataat tataacttaa aattaacgcc acgtaaaata aaacgcaata
    16441 accccaaaaa cgctaaaaat caaaaaaatc catatgaaac tcaaacctat tatcctaagc
    16501 tgaatagcaa ctggtatacc tgtaaaatac gcgaattttc accggattta ccggaacaat
    16561 tatatcaggt actagtcatt aacaaaaaaa caaaggattc cgtttatact tatttgcctt
    16621 actcaaaatc aattgatatt tgcaaaaaca atctggatag agaaatcccc ggcgatttat
    16681 tttttgatga tggcagcaac tttgaaccaa taacaattga catagctgtt cgaaaagttt
    16741 ttccacttcc tcagtatccc atcaccaaca catatttaaa atttgattat accaaaaaat
    16801 ggaatgctga aaggggcgat acattgttat atgtgtatca ggcgcgggtc cacgataaaa
    16861 acaccttagc tgtcattcaa aaaattccaa ttgaaaatgc ccttccattt ccatccaatt
    16921 attttaaaca aaatattgaa accggcgatt tttataaaaa taatcccaag ggtgtaaaag
    16981 atttcagaat aatgacagcc caggtattga acgatagaaa tcaagtgtta tccagttcat
    17041 ttgaccatta tgttttcgac gccgatcaac aaaaatatgt gtatgataag attttgagca
    17101 cgagtaataa tgtagcaata aaagaaaaca ccccggtgat taccaaatgc gaaatcagcg
    17161 tgactgcaaa agaaaaatat tatgattatt tcaaactagt tgatggtgaa tgggtattta
    17221 ctgagcgaaa aacagaatat ttatttgaag ccaatccatt taaatatcaa aaagatgcca
    17281 tcatctggcc tgaaaaatcc aatggttttc ttccggttgt tcaaatgcct tacgacgaca
    17341 aatattatac acacttcgat agttttgcgt tactcaataa tagccgggac accttattac
    17401 tttccttatc tcatagttat agtaaaaaca ctttttcaat accggaaaaa attgcgccga
    17461 aagagaccgt ttatttaaag tattctgaat ggctgcaacc ttcacccggt tacgtttatt
    17521 taattgaaag aaatacctat gtttattttg gcgataagtc gagtgaaata aaaggattta
    17581 attattttct tgctgcaccc ggcactgaag agcgcgataa acaaacaggc aaaattgtgc
    17641 gttataattc taaaattgat accgtttcaa acaccgggat ggtattagaa gtatatgata
    17701 ccggcatgcc aaaagccttt ggacttttta attacaataa tggcaaaaaa gtagacaggt
    17761 gggaaaattg ggatgaaaaa ggcaatcgcc tgcaagcagc tgaatacggt aaaaaattaa
    17821 ctgctttaat tggcaaccct gaccgtttac aggatgaagt tagtatccgc gtaaaaacaa
    17881 aaaccgaatg gaaaaatatg gaatttaaaa gaaatgaaaa tacattcatt ttatttgttc
    17941 caatggatgc cgacagtctt gaaattatta gcggcgagtt tcgtgtcgac tccaaattca
    18001 ctcaatggaa ttccactagt caagccaatt ttacttatta tttattaaat agcaacgacg
    18061 actatattga tcaaatgggt attcgttatc caattacatg gcttgaaaat gaatatttaa
    18121 taaattggga tatcagtttt tacagttatt caaaaccggg cactattgaa aatgaggtaa
    18181 aaagattaca agcaaaatat cccggagttt cgtttatgag ttttgtgtat gcgccacatt
    18241 taattcaggt atttataaat ccaaccactg aatcaaaaaa acaacaactt atacagaacc
    18301 tcattaatga agcttccata ggtagtttat cgcaaggcgc ttcaatgctt aatagtggca
    18361 aacctatgta tcttgaaaca agtgcaacaa taattgtaag tccttatatc agacaagaat
    18421 tagtacaaga aattgcagat aaatacaatg gtaaattaag tcaaatgttt ggaaccggaa
    18481 attattatca attagattta tcatcaacca tgtatgataa atcatatatc aacctactac
    18541 acaccatcca aaaagaaccc aacgtaattg gcataagcgc cggctggaga acagatatta
    18601 attatttaga cgaataacca tcaaaaaaat aaatccatcc cattaaatca atcccaccca
    18661 ccccaatctg ccaccaaaca ggttcctaat tccatcccaa tccatccaga aaatccggac
    18721 attttttcta accaaacgca aaacctttgt gtaagggcgg attcaattcg cccctacaca
    18781 aaggttttac tcacattatc gaaaaaattg aaatttatca ccaaccaaaa attccacacc
    18841 tgtctgccgc caggcaggtt cccaaaaaaa atccgtcccc tatccgttaa acccgtccaa
    18901 tccgcgttcc ctttcaaccc caccctttaa aaaatccgcg cctgtctgcc gctaggcagg
    18961 ttccttttca aacacaccct tcaaaaaatc cgcgccaatc cgcccaatca gcgccctgcc
    19021 ccgcagggtc cgcgttcctt attccatccc aatccctttt taaaacattt tttcccatcc
    19081 gatacaaaaa acccctacct ttgtattgaa atcaaaagtt caggaaatga ataatgtgct
    19141 cttactagca atgccaagcg gaatggaatg gataatcatc ttgtttgctg tattactgct
    19201 tttcggcgga aaaaaaatac cggaactaat gcgtggcgtt ggtaaaggta ttcgtgagtt
    19261 caatgatgct aaaaacaacg tgagccgtga aatccgcgat ggcatgaatg atgaaccaaa
    19321 gaaatctgag gagaaataat tactgcctct tttagatgct ttcaggtttg aattcaattg
    19381 tgcgctctgg attgaaaaat gtccagtgat gtatgcgtgt gggtgatttt gcctgccggc
    19441 aagagttata ctattacctt gggaccagtc ttcaaaaaac aacatacaag ttcggcaata
    19501 attttactgt tccggcaaat attaaaaggc ttcttgccgt caatgtccct ttatttagac
    19561 atcacaacgg aatacctttt gtcgccccgt taatgggcaa ttacacagca tacacctgtt
    19621 gctttctgtg acagaccatc ttcttaaaaa ttattaaaat tttgctgttg ataatgatat
    19681 ttgtgtgtct gagtacaata aaaggctaat tttatagttt tatagcccta acagctatta
    19741 cacaacgacc aaattttaaa ctagggggga aattaaatca atgcactaca tcagaattac
    19801 cgtattatgc cttttgctat ggggtgtggc gaaagcacag gtctctaatt ctaccgtttc
    19861 gacaaacaac ggcatttcat tgaatgctac cgacgaaagt gtgaatgtga tggacagctt
    19921 actggaagct tatttttcga caaatcctgc atggaatttc gaccagagag attatgacat
    19981 gttgggctat gcagctacag atacgcctcg gtttacgcca gagatttatg ctgaacgtat
    20041 cagtaaaatc aactcgccaa ttccttatac ctacaataaa tctgttcagg gtttcctcga
    20101 tttatattgc atgcgccgcc gcacacaggt ttcgaatatg ttgggactaa gccaaaccta
    20161 ttttccaatg tttgaagctg aactcgaccg caaaaacatg cctattgagc tgaaatacct
    20221 cgccattatt gagtctgcgt taaatactca tgctgtatca aaagcaggcg ctaccggttt
    20281 atggcagttt atgaatggca caggaaaatt gatgggttta cgcatcgact cttatgttga
    20341 tgaacgccgc gacccttata tggcaaccgt tgcagctgta gcatatttgg aggatttgta
    20401 taatatttat aacgactggt ttttagctat tgccgcttac aattgtggcc cgggtaatgt
    20461 gaacaaagca ttgcgcaaag cgggtggtgg agataaaact ttttgggata tcgaaaatta
    20521 tctgccaaaa gaaacacgcg gttacgtgcc tgcttttatt gcagcaactt atgtattcga
    20581 atatcataaa gaacacaata tccgtcctgc taaatacgat tatgatttca gcatgatgga
    20641 tacgctcatg attacccata aaatgacgat tgaacaattg gctccttacg ttggattaac
    20701 agcagaagaa attgctttaa acaacccggc gctcaaaact aagacgatac ctggttctcc
    20761 gtatccttat ccactacgtt tgcctatgaa tgcggttgct actttttatg caaataaaga
    20821 ttctttgtat gcatcactca ataaaaagga aacccagaat ttagccactt tagctaaaaa
    20881 tgttgaagaa gttaataatg ctaaagctaa aaccgctact aaaacaacca ctgatgcaac
    20941 aactgcagct acaacaacta ctaccaaaga aataaccgac cctgaggcac cggttacagt
    21001 aagttatact gttaaaaaag gcgataacct gggatatatt tccgattggt tcgactgttc
    21061 cgttgctgat attaaaaaat ggaataaatt aagctctact aaaattgttc cgggtcaaaa
    21121 acttaagtta actgtaccgg caaaacacga agagcaatat gccatgatta acaaaatgac
    21181 atctgcagaa aagcaaaaac taactgatat tcaattgatt agcgctgctc ctgctgaaaa
    21241 agaaccggct accaaagaag taatttatta tactgttaaa ccgggcgata ctttatgggg
    21301 catttcacag aagtatccgg ataataccgt tgataaaata cgtgagctca atgggttaga
    21361 gaaaaatgaa acattaaaaa ccgggacaaa aatcaaattg gttaaataat catcatcata
    21421 ttttaacaaa aggctgcttt cgggcggcct tttttgtttt tagccataaa ccccgttttc
    21481 ggctattgca tataaaaatt acaattcatc agtttttgtg acaaaacggg cgggcgtttg
    21541 cgcgtagaaa caccttaatc tcaatttgct tgattaattt tgaggcaatt accaagctac
    21601 aattactttt aaacgaccca attcagattt atatgcagcg cagatttttg tttgtttttt
    21661 tatgtatgat tttccttagt tcaagccaac tgcttaatgg tcaaaatact attcaattta
    21721 aaaaggaaac aattgactct gtactggaaa cgtttgatca cagcacacgc cttccgggat
    21781 atgcattggc tgttgtacat aaaggcgaag taatttatac cgcgcagcgt ggtaaagcat
    21841 caattgatga taatattaaa attacacaat ctactcaatt taatattggt tcagttgcaa
    21901 aacaatttac aggagcagca attttattat tagaggaagc aggtaaatta tcgctcacag
    21961 atgatattca taaatacata cctgaacttc ctgattatgg acataccatt actttacaac
    22021 acttattaaa tcacacatca ggaattcgcg accatgttga attagctaca ttaatatctg
    22081 aagataaaga tgaattaaat acatttcctg ctatgttgga atggcaaaaa aaataccctg
    22141 cattaaatgg tgcaccggga acatcttttg cctatagcca taccggttat atgatgttgg
    22201 caatggtaat tgaacgtgta tccggactta cttatgctca atttctgcag aacaatattt
    22261 ttactccgct caatatgatg aatacttatg ttgaagaagg aaaaaataat cgtttgcgcg
    22321 atgggtcaac acattatgaa ttaaatcata ccaataccaa agctaaaaaa gccgaagctt
    22381 ataccaatcc attaggagca agcggaataa tttcaacaac agaggactta gctaaatggg
    22441 ataaaaactt tttacaaaat aaattgggca aaggtggcaa tggtttaatc agtaaaatgg
    22501 aaaaatcagg ttcattaaat gatgggactg atgtacatta tggcgcagga ttattaatta
    22561 aatcatatcg cttaaaacca attgcagaac atagtggtgg tcaaggtgag tatttaacgt
    22621 tatatcgtcg gtttttaaat gaggatttat ccattattgt ttgtatgaat tcttatttaa
    22681 tatctccatt cgatatttgt gatgctattg caaataatat catgacttat aaagaacagc
    22741 attggctggt tcaaaaaagt ttgccggata caacctttaa aaaactggaa ggcacttatg
    22801 ctgcagaaaa taatttagtg cgttatgtgt atcgcacaaa aggtcaatta gtaatagcca
    22861 gaatttttga tgaagatacc actgcttatc aattaaccta cacaggcact acgagttccg
    22921 gcgactatgc atttgctgat acacttggtt caactgttat ttttaatgtg gaaggcgata
    22981 aaatagcagg tttaaattgg gatggtggta cttattttat ttgtaacaga tattatacaa
    23041 aagaaaacac cactgtacct ccaaaattaa cttcatttgc aggaaaatat tattgtgcag
    23101 cgctcgacaa aaaagtacgt atccaatact tttctaaaaa ggatgaatta accatacacc
    23161 cgtttccgtt tatatcgtat aatttaattt caaagggtgg cggaatttat gttgttgaag
    23221 accaacctta cattgtcaat tttaataaaa atgaatttat gattggcaac gattggattt
    23281 ttaatatcaa gtacgacaaa aaaataaaac ctaaaaagga gaaaaaagaa aagcctgaaa
    23341 aagagaaaaa aggtttattt gggtggtttg gcaaagacta attgcacact atgaaatttt
    23401 ctttttttgt tattggttta atctgctggt tcgatacctt cgctcaattt gaaaccgata
    23461 ttacttatcg tcagcttgat tccattattt atggcaaaaa tccagatact gaagctcccg
    23521 gcatgggcgc tattgtaatt aaagatgatt caatagttta ttttacttcc accggttatg
    23581 ctaacacaaa acgcaaatta ccaattggct tacacactca atacaatata ggttctatca
    23641 caaaaatgtt tactgctact gccattttat tattggaaga agaaggtaaa ctgcgaagaa
    23701 cagatgaaat tcatcaatac attcctgaat tacccgagta tccatttcca attaccatca
    23761 atcatttatt agggcatacc agtggcattc gcgaccattt tgaagttgca cgatttttat
    23821 ttaattacaa taacaaactg gtttcgtttg aagggatgct cgactatcaa aatgaatttc
    23881 ccgaactcaa taccaacacg ggtgaatctt ttgcttatag caataccgga tacatgttgt
    23941 tggcaatgat tattgaaaaa gtgagcaacc agtcatacga atcgtatttg gcagaaaata
    24001 tttttcaacc actgggaatg gaaaatacat ttgccacccg cgaaaaacaa aaatatttgt
    24061 cggatgggac tgctaattac ccacagaaaa aaaatggaaa atttaaaggg ccgcttgatt
    24121 attatgatgc cttaggtgca acaggtgttt atgccacatt gtatgatatt tatttgtggg
    24181 ataaaaattt ttaccataat aaattaggga aactatcgca aagtttaatc gacactttac
    24241 aacattcatt tgccttaaat tcaggtgagt cggtaaacta tggttgtgga attatcgaaa
    24301 aaaaatatcg gggcaaagtc gcttacgaac atagtggtgg ttggggatat tatttaacgc
    24361 aataccgcag atttccaaat gaacatattt cggtaatagc ctggaataat tcagacaact
    24421 attcaccatt tactac
//
LOCUS       DAORKW010000007        24001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_10_length_24001_cov_5.514909, whole genome shotgun sequence.
ACCESSION   DAORKW010000007 DAORKW010000000
VERSION     DAORKW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 24001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24001
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10_length_24001_cov_5.514909"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1990
                     /locus_tag="PLB46_00725"
     CDS             182..1990
                     /locus_tag="PLB46_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF75062.1"
                     /translation="MKYCVAAFLIITFYTTKAQFVLNGDAVSLGGDCYQLTEETTFSA
                     GSIWYETLINLEENFEINFQLNMGDLDASGADGMYFVLQPVGTGLGDAGGGMGYLGIT
                     PSLGIEFDTYQNGDYGDPAYDHIAIQYNGELDHTGIYNLDGPTQIVAGEPNIEDGEYH
                     FVKITWDAGDQHLQVFVDCEYRVGFITEVFDIIETIFAGDPEVYFGFTAGTGSLYNNQ
                     IICFEYTTEIDALEDVTICPGDSVQFNLPEDFVTYEWSPAEGLSDTDIKNPWASPDEN
                     TTYTVILTDACGVEIYDTVNVIVNTPDFVNLGPDLTLCEGQLWTFNVLTPGATYLWQD
                     GSTLPTLTVDENGTYSVIVTDGNCVDGDTVSVNYVPAPDISLPEDTVICGLENTYTID
                     VTTPGANYLWQDGSTESTFTITDDGLYYVTVTVGGCSDKDSVLVNYSVQPSVDLGPDA
                     SLCLGTTILLEAGSETFDYVWQDGSTDNTFLVSTPGTYSVSVIINGCEATDEIVIVPD
                     PCICKVTAPNIFSPNGDGFNDNFSQIDCYDLLSYELTVYNRYGEIVFMTSKMDGAWNG
                     QFEGKDCEMGTYVYVISYTRPTGETGVTSGNVILVR"
     gene            2125..5736
                     /gene="dnaE"
                     /locus_tag="PLB46_00730"
     CDS             2125..5736
                     /gene="dnaE"
                     /locus_tag="PLB46_00730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRF75063.1"
                     /translation="MPKFSHLHNHTMFSLLDGASDISRLYKKAVKDNMPALAITDHGN
                     MFGAFQFVAEAYKHKNEDGSLKVKPIVGCEFYLVADRHRKQFSREEKDIRYHQVLLAK
                     NEIGYKNLVKMCSLGYMEGMYGKYPRIDRTLIEKYHEGLIATTCCLAAEVPRAIMKKS
                     KEEAEATFKWWLDIFGEDYYIELQRHDIPDQIYVNDTLLQYAKKYNVKVIATNDAHYV
                     DQADANAHDILLCINTGEKQATPKMKDFGDDDMMVKGKRFAFYNDQFYFKTQAEMTNL
                     FEDVPQAIDYTNEVVDKIQLLDLKRDILLPAFPIPAEFKIHQDDVLNQWEYLKHLTFE
                     GAKKRYGEVDAEHEERINFELFTIKTMGFAGYFLIVMDFIRAGRDMGVFVGPGRGSAA
                     GSVVAYCIGITNIDPMKYNLLFERFLNPDRKSMPDIDTDFDDDGRQRVIDYVVDKYGK
                     NQVAQLITYGTMAAKTSIKDVARVMDLPISESNALSKFVPERPGISLNRLIYAPLSGD
                     GSLADKENLSPDEMANAKTLRGILEDQKDVRSNILKEALVLEGSVRNTGVHAAGLIIA
                     PSDLTDLIPIAVAKDSNLYVTQFEGEVIESAGVIKMDFLGLRTLSIIKTALNLIKQNH
                     GVEIDIDTIPLDDVTTFELYQHGETNGTFQFESPGMQKYLKELKPDKFEDLIAMNALY
                     RPGPLEYIPTYIKRKHGRESIVYDLPEMEEILKETYGVTVYQEQVMLLSQRLANFTKG
                     DADTLRKAMGKKQKDVLDKMKSKFITGCEKNNFAPKVCEKIWTDWEAFAQYAFNKSHS
                     TCYAFVAYQTAYLKAHYPAEYMSAVLTHNQGNIDKVSFFMDECRRMGVPVLPPDVNES
                     DVTFAVNKTGQIRFGLTAIKGVGEAAVIELIRERKDTGIFNGLYDLTRRVNLRSVNKK
                     TLESLILAGALDSFGLNRSQYFAVAKNETANVIENAIKYGNNFTGNVVTSQNTLFGDS
                     NDIEITEPKIPDFPEWNTIEKLKKEFEVIGIYLSGHPLDDFKIEVDAFKTCNINELEK
                     FTDKGEVAIVGYIASTDTKIGKSGDKYTIFSLEDFSDHIELGLFKNNYIKYKNYVDQT
                     GNVLYVKGNYQTDRNDPERKRFWVSDIQLLSEMREKKSMKITLNIALSSLNKEFIKSI
                     DEVIKANPGKDRLVLKIVEETENFDVLFRTQTACVKADKPVLNTLGKFGEVSLKVAG"
     gene            5831..6148
                     /gene="trxA"
                     /locus_tag="PLB46_00735"
     CDS             5831..6148
                     /gene="trxA"
                     /locus_tag="PLB46_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRF75064.1"
                     /translation="MALEFTDANFKQTVLDSEKLSVIDFWAEWCGPCRVVGPVIEELA
                     KDYDGKVNIGKVNVDFNPEISMMYGIRNIPTILFVKGGQVVDKQVGAVPKSVLDAKVK
                     QHL"
     gene            6218..7153
                     /locus_tag="PLB46_00740"
     CDS             6218..7153
                     /locus_tag="PLB46_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRF75065.1"
                     /translation="MNGQLISLKDLRQVENLELIAKQVVEGFILGLHKSPFHGFSVEF
                     AEHRIYNPGEATRHIDWKVFGRTDKLFTKKYEEETNLRCQIVIDASSSMYFPEPDKKA
                     TGLHVNKLIFSTICASAIIHLLKLQRDAAGLTIFTDDLLVHTKSGSSNVHHKLLQTQL
                     ESFIGKSSRDVKTDAGKCLHLIAENIHRRSLVVIFSDMFEDATKADELFSALQHLKHN
                     KHEVVVFHVTDKKHELDFAYDNRPYVFVDMETGEKVKLRSNDVKSFYTEQMEQYIKTL
                     KLRCHQYHIDFVEADMNAGFKEVLLPYLVKRQKQR"
     gene            7388..8149
                     /locus_tag="PLB46_00745"
     CDS             7388..8149
                     /locus_tag="PLB46_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF75066.1"
                     /translation="MLLNFKTLGQGKPLIILHGLFGMLDNWQSVAKVLEHDFTLYLID
                     QRNHGKSPHTAQHSYKLMAEDLRDFMLREGIPQSNILGHSMGGKTAMQFALDFPEMVE
                     KLIIVDIGIKRYDSGHDHIFEALQSVQLNNIKSRNDAELQLSQFIHQQGVQQFLLKNL
                     TREPDGTYHWKFNLAALNDNYENNILSAITSDMPFEGATLFIRGENSDYITFEDWPQI
                     LQLFPNANLSTVNDAGHWVHADNPQSLIKIIQEFL"
     gene            8280..9275
                     /locus_tag="PLB46_00750"
     CDS             8280..9275
                     /locus_tag="PLB46_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75067.1"
                     /translation="MKKLLLITFLLTPFVALIAQPTITSSINGGIGDEFNYIDVQTEG
                     LNPGPAGENVTWDFSDIIESGAEYGYTWVDVASTPDGGDFPGANIAADNGPGTYSYFK
                     VTASEFTNYGVSAAGSIVVYQDPEEIFVFPMTYGTTNTDDLFSEFINGIAFERSGTVT
                     MEVDAYGTLILPSGTYTDVLRVKVKEDYMDEADLLPTPIIYDFTNYYWLKAGVKGPLF
                     QYFDLVTDGGFPFEDQQAAYAENVAVGIAEHNTIQSLTIFPNPVKEVATIIVPEGINS
                     AAISIYNNLGQVVTSTVETVNHNTITLPVGYLQSGIYMIALTEGDNTYNAKMIKR"
     gene            complement(9389..11137)
                     /locus_tag="PLB46_00755"
     CDS             complement(9389..11137)
                     /locus_tag="PLB46_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75068.1"
                     /translation="MKKTMFLFLLLPFLINAQVTLDWQQFEGGLSLATDDTDNLYSIY
                     YEYNPAGDIYLTKHDAAGNYIWTTSYDNTDNTRHEVATWVETDNDNNIIVTGTIRSGY
                     ASPVNAASVVMKFDPSGNLLWRNVYETSFDGSYTVKCLVDADNNIYVLGLGSNGVGMV
                     TKVKKFAADGTAIWNYFNVAGIGAPTNFKFTPDNKIVIIGRGTVGSLNGYAKIDLDGN
                     ELWSVAGVYSLTPGDAAGDLDGNTYIINQDWFGGSGSVLTKLSPAGSIAWTHTNEMVG
                     TKVEVGTDNYPVIGGFPNAGTFGVAMMKYTNTGTPIWTNIDADGPDQALLLHNIMKMD
                     IANNIYFGAGTLFQQAVCKVNADGTHDWIALASSGYNTDFEFGNDYSLFMLGGIALAH
                     FIQDPIITCDAPEGLFTNNITTVKARLNWTVEPGAFQYEVWYKKATAINWKKKFVPGV
                     NNKLNLKNLQCGTNYVWQIRTICDTVGVDEISAWSPLQSFTTLACRAGDNMEGDGSII
                     SLYPNPASTNLTIVINSDIQSIQIFDMTGKLMFNQNIAAAESIQLNISEWNSGIYLVQ
                     TVGTLGTTTEKLVIQH"
     gene            complement(11404..13203)
                     /locus_tag="PLB46_00760"
     CDS             complement(11404..13203)
                     /locus_tag="PLB46_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75069.1"
                     /translation="MSLLGMVGTIFSQVTEDWVSPDFYYGLDGSAIVVDEADNTYALS
                     DIFNGDIYLTKRNATGTILWTATYDNTEPSEWEMAGDVAIDINGDAIVTGYTNVGFGS
                     EWYPLQMVTMKFSGVDGSLIWRKTYATDVRYRGRKLLTDAEGNIYVGGDVNAWMIYHG
                     EVGTMMVKKYDTDGNDIWTVIEDAAGNDFGGTMLKMQFAPDGNIVIGAYGTSLAKIST
                     AGTVLWHTSGITNGLVSFDIDPLGNIFTASHGSFGTPPFISSDFVVSKFNSSGTLLWT
                     NNYDFGHEEFSRQIVCDNSGGAYITGYGDLYFDWYTFKVDGSGVMLWSQVYDEHTGND
                     EIPKMMIKDEYDNIYITGQGGPWPGYFWLSLVQMVTIKYTPAGVEEWVALHTDYSNTG
                     SALCLASDNSIFALGQQYAVVLHYNQVMAVSCSTPTGLFTNNITSSQARFNWSFVPEA
                     VQYEVWYKKAIAPTWKVKFVPSTKNKLNVKSLSPNKAYVWKVRTICADGLVSDFSPEQ
                     TFNTLPLREGELNTTAVVSIYPNPASNIVYIQSENAELKSITFFDMSGQVVLSTTDDP
                     SEGINTSSLTAGMYLIRIETTDEVINQQLIIHN"
     gene            complement(13597..14721)
                     /gene="hppD"
                     /locus_tag="PLB46_00765"
     CDS             complement(13597..14721)
                     /gene="hppD"
                     /locus_tag="PLB46_00765"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRF75070.1"
                     /translation="MNTLELTSTQTTTDFLPLQGTDHIEFYVGNAKQSAYYYQSAWGY
                     ELVAYAGLETGVKDSASYVLQQDKIRIVLTTSFDPNSEISEHVRKHGDGVKVLALWVD
                     DATKSYEETVKRGAKGVIAPTTLKDEFGEVVVSAIQTYGDTIHKFVERKNYKGVFMPG
                     YQTKKSNVPVKPVGLKYVDHCVGNVELGKMNEWVKFYEDVMGFKLLITFDDSDISTEY
                     SALMSKVVSNGNGYVKFPINEPAEGKKKSQIEEYIDFYKGAGVQHIAIATDNIIETVT
                     ELRARGVEFLYVPEVYYEDVLDRVGHIDEDFDELKKLNILIDRDDEGYLLQLFTKPVE
                     DRPTVFYEIIQRHGARSFGKGNFKALFEAIEREQELRGTL"
     gene            complement(14837..15184)
                     /locus_tag="PLB46_00770"
     CDS             complement(14837..15184)
                     /locus_tag="PLB46_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRF75071.1"
                     /translation="MSNEEKNLIVDLTFEFSLLIIDYTEELEKLKKNRMAWQLFDAGT
                     SVGANVNESQNAESRADFIHKMKIAAKEGSESQYWLRLCKHAKNYPECDDLLERIVVI
                     NKILSKIISSSKK"
     gene            complement(15354..16511)
                     /locus_tag="PLB46_00775"
     CDS             complement(15354..16511)
                     /locus_tag="PLB46_00775"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRF75072.1"
                     /translation="MPFYNKSGNIPEKRHIQFRKPDGTLYHEELFSTEGFSNTYSLLY
                     HLYPPTKIKQVGEAYSVEPKVVLSKQLKHRSLKGFKIKPVDDYLESRKAVLVNSDLHI
                     SLAAPRKSMTDYFYKNVDADEMIFVHEGTGTLKTMYGDIPFEYGDYVIIPRGTLYQIT
                     FNDENNRLLIVESFSPIEFPKRYMNRVGQLMEHSPFCERDIKTPQNLKTHDEQGDFYF
                     KIKKQGIIYPYHYANHPFDLIGWDGYVYPWAFSIHNFEPITGRVHQPPPVHQTFEAHN
                     FVVCSFVPRMYDYHPLGIPAPYNHSNIDSDEILYYVDGDFMSRKSVERGQITLHPGGI
                     PHGPHPGTYEGSIGKKETKELAVMIDPFRPLQLTEAALEIEDTEYYLSWLS"
     gene            16852..18390
                     /locus_tag="PLB46_00780"
     CDS             16852..18390
                     /locus_tag="PLB46_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75073.1"
                     /translation="MKSYYIIAICLLFLQSLIVVAQDIQVNTSLLEDKFSADASKNLG
                     GDETGFYIASFSSKYKFEVGFNATGSQTTLIIQKYSNDLKLLAEKAIPQDENESLEWP
                     ILFNGDIYSISRKYSKKTSSWNVYAQKLEPVKLYNSGAAALLFNVKQEDDQLYLSLDM
                     VKSADNKFIAFHAFPMQSIGRDYGDKLGNRFNSNMTTIAVFGEGLVKQWEKNVVIPST
                     DDKYTPTQVSVDNNGDVYILGWRFIEGAKATKPNTIRAITSLHQISNKGAQMKNIDLV
                     QQGVNILDALMAFDKAGNIIVAGVCSAADEADEMDAFYAFTVDKSALSIASKSVKNFT
                     QDFLDKIKLDKAFRVKLSLDELLLMDNGDFIFIIEEFAVSNTQSGTYFSCLNLITLGV
                     DSNAEIVWMNAIPKKQFSIETDFTSYNFMQTGDKLYLFYNGNSENLNAKNYRELEDYK
                     IKNSQIMMVTINSDGSYKKVVLPGDISAYHLRVTMSQQSTDKKFIFFARDGKKEYIGA
                     IQVN"
     gene            complement(18420..19598)
                     /locus_tag="PLB46_00785"
     CDS             complement(18420..19598)
                     /locus_tag="PLB46_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HRF75074.1"
                     /translation="MEPSPKIDFNDTEIAFSGKSNKELKWEYWLFKVMNNPVMTNTLT
                     TLASWTLNLHLPFKWAIKGTVYQQFCGGETLEETIPVLNKLEQFHIGAIIDYGVEGKE
                     SEAEFERTKNELIRIIQFASGKKNIPFISSKVTGVTSFALLEKLNSKTTLTAEEEKEK
                     AKLYSRMDQIAAAAAQNHIGLFIDAEESWIQDAVDDLTFELMRKYNKQKAVVFATPQM
                     YRHDRLAFSKRALEDARQHNYILGLKPVRGAYMDKERKRAEEMNYPSPIQPDKNATDR
                     DYDAVILFCLENMDRIAVCAATHNEKSSLLLASEMQKRNIPANHPHFYFSQLYGMSDQ
                     ITYNLAKAGFNVTKYLPYGPVKDVIPYLIRRAQENKSVSGQMSRELNLIDKEIKRRGL
                     "
     gene            19683..20972
                     /locus_tag="PLB46_00790"
     CDS             19683..20972
                     /locus_tag="PLB46_00790"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HRF75075.1"
                     /translation="MRAFGLFLMLGLSMPAIAQDYTSYFTGDTTDVTVTVQKGTVLMG
                     GASENDNAIRWFLERAGGGDILVLRTSGEDGYNDYFFADLGVDVNSVETIVCNNKDAS
                     FNPYVLRRVAEADALWFAGGDQWEYIDYWRDTPMEDAFNLLINEKQITIGGTSAGCAI
                     MGQAYFSAENGTIISNTALHDPYHNLLQLGNDDFLNNPATANLITDTHYDNPDRRGRQ
                     VTFLARLMTDFGMPFYGIGVEEYTAVCIDEFNIAHVYGSYPGDDDFAYFLQVNCYTPS
                     GPEVCADGDLLTWDRNDAAIKVIKMAGTNSGSGMVDLNDWKTASGADFTWENWWVENA
                     VLKVTTDATEIDCTTPIAQSNNINTSLAINIEQDVLHIYTDMLLEAYKLYTITGQLIQ
                     SGKLLNNNIQIQQLKNGTYLLVVIDEQQNTNYIEFVK"
     gene            complement(20985..22220)
                     /locus_tag="PLB46_00795"
     CDS             complement(20985..22220)
                     /locus_tag="PLB46_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF75076.1"
                     /translation="MRPKVPNFYLSKIPVITVLLMIWHFIGGIEVKCQNFIPNGDFEE
                     YDTCPNKLGQLKLGEPWFNATYPASEYFNTCSINDTASVPTNYFGIQQAQSGSGYCGM
                     YVAQTGGPPYREFIEAKLTSPLVANAAYNLVWYASVADISSCFPASICVYLTSDSMIN
                     YTTTSFLTAATLIEQQFCNPENTIFNDTSAWVKINCCFIATGEERFIVIGNNYSDLFI
                     GCSGDPGVFQAAYLYLDNISLIEMPTQTVHFDTAVCKGQPVQINLHKLIEEPGNSNPI
                     FVWDDGYIGAERLLVHDKIYTITINNGCATDTAIIQLHYLEDCPEVFYMPNAFSPNHD
                     GINDNFRITNENITILNFEIYNRYGKQIVSVYDYLTGWDGNVDGKPADIGVYIYHLWY
                     RTNISETFHEKKGYVTLIR"
     gene            22351..>24001
                     /locus_tag="PLB46_00800"
     CDS             22351..>24001
                     /locus_tag="PLB46_00800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF011531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRF75077.1"
                     /translation="MQSFVVSARKYRPSSWHDVIGQDQVVVTLKNAIKNNHLAQSFLF
                     CGPRGVGKTTCARILAKTINCENIQENGEACNTCESCSSFNENASFNIHEIDAASNNS
                     VEDIRSLVDQVRYAPASGKFKIYIIDEVHMLSASAFNAFLKTLEEPPLYVKFILATTE
                     KHKIIPTILSRCQIFDFNRIRIKDMTQQLEIICKNEGIDCTDEAMHLIAQKADGAMRD
                     ALSIFDRITSFSGKSITAKDVIENLNILDYEYFFKITDAFVVEDVATALVIFDEVVNK
                     GFDGDIFINGLAEHFRNILVCKDEATLKLLDTSETVIERYKTQSNLISASFLLSGLNI
                     LNSTDVNYRASKNKRLHVELALMKLCYVHSAVSTLSSGSMEKKTPDLNEKRPNAAIKP
                     IASAVKTTETNNISDISTVIEEPVKKVRVENSTIITPQLESVEPVVIPKKTDIRQAIK
                     KEEDEKSAALLTKTVEEPLKVISPEMFFIVWKQYSDFIRKDQNRVAIIMDAAAVSINE
                     TNNIVVRLESNLEANIMKEMQNDLREFIKDGLENNQFDISFI"
BASE COUNT         7671 a         4194 c         4469 g         7667 t
ORIGIN      
        1 atggttgagt ccatcatccc ggtcccaatc cacactccgg attgcccgaa agcggctaaa
       61 gaagcaaaaa gaagtaatag agatatgaat ttcattgggt attcctaacg ttaaacgtaa
      121 aaatctgttc aaaattatcc atattatgga ttagatttac ctttgcaaaa aaattactca
      181 aatgaaatat tgtgttgccg cgtttttgat aattacattt tataccacta aagcccaatt
      241 tgtattgaac ggagatgccg tgagtttggg cggggattgt tatcaattaa cggaggaaac
      301 aacctttagc gccggttcta tctggtatga aacgctcatt aacctggaag aaaattttga
      361 gattaatttt cagctcaaca tgggcgattt ggacgcttca ggtgctgatg gtatgtattt
      421 cgttttgcaa cctgtaggaa caggtcttgg cgatgccggc ggtggaatgg gatatttggg
      481 aattacacct tcactaggaa ttgaatttga tacgtatcaa aatggagatt atggcgaccc
      541 ggcgtatgac catattgcta tccaatataa tggtgaatta gatcacacag gaatttataa
      601 tcttgatggc ccgacacaaa ttgttgccgg tgaaccaaat attgaagatg gggaatatca
      661 ttttgtaaaa ataacctggg atgcaggcga tcaacattta caagtatttg tagattgtga
      721 ataccgagtt ggatttataa cagaagtatt tgatatcata gaaactattt ttgcgggtga
      781 tccggaagtg tattttggat ttactgctgg aacaggttca ttatataaca atcaaattat
      841 ttgtttcgag tatactacag aaattgatgc attagaagat gttaccattt gtccaggtga
      901 tagcgttcaa tttaatttac ccgaagattt tgttacttac gaatggtcac ctgctgaagg
      961 attaagtgat acagatatta aaaatccatg ggcatcaccg gatgaaaata ctacctatac
     1021 cgtaatttta actgatgcat gcggagtgga aatttatgat accgtaaacg tgatcgtaaa
     1081 tacgcctgat tttgtgaatt taggtccgga ccttacgttg tgtgaaggac aattatggac
     1141 atttaatgtg ctcactcccg gtgcaactta tttatggcag gatggttcta cattacctac
     1201 attaactgtt gatgaaaatg gtacttattc tgtaattgtt actgatggaa attgtgttga
     1261 cggtgataca gtgtccgtta attatgttcc cgctccagat atttcacttc ctgaagatac
     1321 tgtaatttgt ggattggaaa atacatatac tattgatgtt accactcctg gtgctaatta
     1381 tttatggcag gatggttcta ctgaatctac ttttactatt acggatgatg gtttatatta
     1441 cgttacagtt acagttggag gttgtagtga taaagattct gttttagtaa attattctgt
     1501 gcaaccatct gtggatttag gcccggatgc aagtttatgt ttaggcacaa ccattttact
     1561 agaagcaggt tccgaaacat tcgattatgt ttggcaggat ggttctactg ataatacttt
     1621 tttagtgagc acaccgggta cttattcggt atcggtaatt attaacggct gcgaagcaac
     1681 agatgaaatt gttattgttc cggatccatg tatttgtaaa gtaactgcac caaatatttt
     1741 ttctccaaat ggcgatggtt ttaatgataa ttttagccag atagattgtt atgatttatt
     1801 gagttatgaa cttaccgttt acaaccgtta tggtgaaatt gtattcatga catccaaaat
     1861 ggatggcgcc tggaacgggc aatttgaagg taaagattgt gagatgggca cttatgttta
     1921 tgttatcagt tacacccgcc caactggcga aaccggtgtt accagcggta atgttatttt
     1981 ggtgagatga cgtgtacatg cagctaatac cacgtggtag caggagtata taattatcca
     2041 aaataattat tcacatacgt tagtaaatta tcttccctaa ttatcgtaat ttaagggtta
     2101 gaaaatttaa tttcgccatc ctaaatgcca aagttttctc acttacacaa tcatacgatg
     2161 ttttcgctgc tcgacggtgc cagcgatatt tcccgtttat ataaaaaggc agttaaggac
     2221 aatatgcccg ctctggctat taccgaccat ggtaatatgt ttggtgcttt tcagtttgtt
     2281 gctgaagcgt acaaacacaa aaatgaagac ggttcattaa aggtaaaacc gattgtaggt
     2341 tgcgaatttt atttagtggc cgaccgtcat agaaaacagt tttcccgcga agaaaaagat
     2401 attcgttacc atcaggtatt attggcgaaa aatgaaatcg gatataaaaa tttggtgaaa
     2461 atgtgttcgt tgggatatat ggaagggatg tatggaaaat atccacgtat cgacagaaca
     2521 ttaattgaaa aatatcatga aggtttaatt gccactactt gttgtttagc agctgaagtg
     2581 cctcgcgcca tcatgaaaaa aagtaaagaa gaagctgaag caacatttaa atggtggctg
     2641 gatatttttg gagaagatta ttatatcgaa ttacaacgtc acgatattcc ggaccagatt
     2701 tatgtgaatg atacattatt gcaatacgcg aaaaaatata atgtaaaagt tattgcaacc
     2761 aacgatgcac attatgttga tcaggctgat gccaatgcgc atgatatttt attgtgtatt
     2821 aataccggcg agaaacaggc aacgccaaaa atgaaagatt ttggtgatga tgatatgatg
     2881 gtaaaaggca aacgttttgc tttttataac gaccagtttt attttaaaac acaagcagaa
     2941 atgaccaact tgtttgagga tgttcctcag gcaattgatt ataccaatga agtagttgat
     3001 aaaattcagt tactggattt aaaacgtgat attttattgc ccgcgtttcc cattccggca
     3061 gaatttaaaa ttcatcagga tgatgtatta aatcagtggg aatatttaaa acacctcaca
     3121 tttgaaggtg caaaaaaacg atatggcgaa gttgatgcag aacatgagga gcgcatcaat
     3181 ttcgaattat ttactatcaa aacaatggga tttgccggat attttctcat tgtaatggat
     3241 ttcattcgcg ccggtcgcga tatgggtgta tttgttggac ctggtcgtgg ttctgctgcc
     3301 ggttcagtgg tagcgtattg catcgggatt actaatatcg acccgatgaa atacaatctc
     3361 ctctttgaac gtttcctcaa tccggacaga aagtcaatgc ccgatattga tacggatttt
     3421 gatgatgatg gccgccagcg cgttattgat tatgtggtag ataaatatgg taaaaatcag
     3481 gttgcacaat taatcactta cggcaccatg gctgctaaaa ccagtatcaa agatgttgcc
     3541 cgtgtaatgg atttgccgat atcggaaagt aatgcgttgt caaaatttgt ccctgagcgt
     3601 ccgggtatat ctttaaacag attaatttat gcgccactta gtggagatgg tagtcttgca
     3661 gacaaagaaa atttatcgcc agatgaaatg gctaatgcca aaacattaag aggtattctg
     3721 gaagaccaaa aagatgtgcg cagtaatatt ctgaaagaag cgctcgtatt agaaggttcg
     3781 gtaagaaata ccggtgttca tgctgcggga ttaattattg cgccaagcga tttaaccgat
     3841 ttaattccaa tagcagtcgc taaagactcg aatttatatg tcacccagtt tgaaggtgaa
     3901 gtaattgaaa gtgccggtgt aattaaaatg gactttttgg gattgcgtac cttgtccatt
     3961 attaaaactg cgcttaatct gattaaacaa aatcatggag tggaaattga tattgatact
     4021 ataccacttg atgatgttac cacatttgaa ttatatcagc acggagaaac aaacggtaca
     4081 ttccagtttg aaagtccggg catgcaaaaa tatttaaagg aattaaagcc cgataaattt
     4141 gaggacctta ttgcgatgaa cgcattatat cgcccgggtc cgttggaata tattcctacc
     4201 tacatcaaac gtaaacacgg tcgtgaaagt attgtgtacg acctgcctga gatggaagaa
     4261 attctgaaag aaacttacgg cgttactgtt tatcaggaac aggtgatgtt actttctcag
     4321 cgattggcca atttcacaaa aggtgatgct gatacactga ggaaggcaat gggtaaaaaa
     4381 caaaaagatg ttttggataa aatgaaatcc aaattcatta ccggttgtga aaaaaataat
     4441 tttgcaccta aagtttgtga aaaaatttgg accgactggg aagcatttgc gcaatatgca
     4501 tttaataaat cgcattctac ctgttatgca tttgttgctt atcagactgc ttatttaaag
     4561 gcacattatc ctgcagaata tatgagcgct gtattaacac ataatcaggg caatatcgat
     4621 aaggtgagtt tctttatgga tgaatgtcgc agaatgggcg ttccggtatt acctcctgat
     4681 gtgaacgaaa gtgatgtgac atttgcagtg aataaaacag ggcaaattcg gtttgggtta
     4741 actgccatta aaggagtagg agaagcggca gtgatagaat taattcgcga aagaaaagat
     4801 accggtatat ttaatgggtt gtatgattta acacgcagag taaatctgcg cagcgtaaat
     4861 aaaaaaacat tagagtcgct gattttagcc ggcgctttag atagttttgg attaaaccgc
     4921 tcccaatatt ttgcagttgc caaaaatgaa acagcaaatg tgattgagaa tgcgattaaa
     4981 tatggcaata atttcacagg gaatgtggtt acttcacaaa acaccttgtt tggcgatagt
     5041 aatgatattg aaatcaccga accaaaaatt ccggacttcc ctgaatggaa tacgattgaa
     5101 aaattaaaaa aagaatttga ggttattggt atttacctga gcggacatcc acttgatgat
     5161 tttaaaattg aggtagatgc atttaagact tgtaatatta atgaactcga aaaatttacc
     5221 gataaaggtg aagttgccat tgtaggctat attgcatcaa cagatacaaa aattggtaag
     5281 tccggtgata aatacacgat attttcttta gaagatttta gtgaccatat tgaactcgga
     5341 ttatttaaaa ataattatat caaatacaaa aattacgttg accaaacagg caatgtatta
     5401 tatgtaaaag ggaattacca gacagacaga aacgacccgg aaagaaaacg gttttgggta
     5461 agcgatattc agttattaag cgaaatgcgc gaaaaaaaat cgatgaagat tacgctgaat
     5521 attgcacttt cttcgctgaa taaggagttt ataaaaagca tcgatgaggt gattaaggcc
     5581 aatccgggaa aggacaggtt agtattgaaa attgtggagg aaacggagaa ttttgatgtg
     5641 ttgttccgga cccaaactgc ttgcgtgaag gccgataaac cagttttgaa caccttgggg
     5701 aaatttgggg aggtaagttt aaaagtggct ggttaaattt gtgttttatt agcgaaattt
     5761 gaccaccaag gaactaaatc cttgtctatt tgttctaaat catacaaacc gaataaaaca
     5821 atttaatact atggcattag aatttaccga tgcgaatttc aagcagacag tgcttgacag
     5881 cgaaaaacta tcagttattg acttttgggc agaatggtgc ggtccttgcc gcgttgttgg
     5941 acccgttatt gaggaactgg cgaaagatta tgatggaaaa gtaaacattg gaaaagtaaa
     6001 tgtggacttt aaccctgaaa tttctatgat gtatggcatc cgcaatatcc caactatcct
     6061 tttcgtaaaa ggtggtcagg tagtggacaa acaggtaggt gctgtgccta agagtgtatt
     6121 agacgctaaa gtaaaacaac acttataatt gttaaacata cttaattaaa gccttccttc
     6181 gtttgacgga aggctttttt agttttaatt tgcagttatg aacggtcagt taatttcact
     6241 caaagactta cgccaggtcg aaaacctgga actcattgcc aaacaggtag tggaagggtt
     6301 tattttgggg ttacacaaaa gtccgtttca tggcttttca gtagaattcg ccgaacacag
     6361 gatttacaac ccgggtgagg ctacaaggca tatcgactgg aaggtatttg ggagaacaga
     6421 taagttattc acaaagaagt atgaggaaga aaccaatttg cgttgtcaga ttgtgattga
     6481 cgcctcttcg agtatgtatt ttcctgaacc cgataaaaag gcaacaggtt tacatgtcaa
     6541 caagctcatt ttttcaacca tttgtgcaag tgccattatc catttattaa agctgcaacg
     6601 cgatgctgcg ggattaacaa ttttcaccga tgatttattg gtgcatacca aatccggaag
     6661 cagcaatgtt catcataaat tattgcaaac acaattagaa agttttattg gcaaatcatc
     6721 gcgcgatgtt aaaacggatg ccggtaaatg tctgcattta attgcagaaa atattcaccg
     6781 cagaagtttg gttgtaattt ttagcgatat gtttgaagac gccactaaag cagatgaatt
     6841 attttcagca ttacaacatt taaaacacaa taaacatgaa gttgttgttt ttcatgttac
     6901 cgataaaaaa catgaattag attttgctta cgacaaccgt ccatatgttt ttgtagatat
     6961 ggaaacaggg gagaaggtga agttacgaag caatgatgtt aaatcgtttt acaccgaaca
     7021 gatggagcaa tatatcaaaa cattaaaatt gcgatgccat caatatcata ttgattttgt
     7081 ggaggcagat atgaatgccg ggtttaaaga agttttgttg ccctatttag taaaacggca
     7141 aaagcagcgg taattaattt cagctagatt ttttgatttt cgatttttcg tgggatgtca
     7201 aattgtcacg cagtggattt ttgcagaata atttggtggg ataatgattt tagttgaagt
     7261 taaactgttg tgatttgttt ttttatcgca aagaccttcc aggttttata acctggaagg
     7321 ttttagagct gtctgccaaa attgcagtcc caaaaatttt cattttaact taaatatctt
     7381 tgcaaaaatg ctgttaaatt ttaaaacttt aggtcagggg aaaccactga taattttgca
     7441 cggattattc ggtatgctgg ataattggca atcggttgca aaggtattag agcatgattt
     7501 tactttgtat ttaatcgacc aacgcaatca tggaaagtcg ccacatactg ctcagcattc
     7561 ttataaatta atggccgagg acttacgcga ttttatgttg cgcgaaggaa ttccacaatc
     7621 caatatttta ggccattcta tgggcggtaa aactgctatg caattcgcac tcgattttcc
     7681 cgaaatggtt gaaaaactga ttattgtcga cataggtatt aaaagatatg acagcggtca
     7741 tgaccatatt ttcgaagcat tacaatcagt tcaactgaat aacattaaaa gtcgtaatga
     7801 tgcagaattg caactctctc aatttattca tcaacagggt gtccaacaat ttttgttaaa
     7861 aaacctcacg cgagaaccgg atggaacgta tcattggaaa tttaatttag cggcattaaa
     7921 tgataattac gaaaacaata ttttatcggc aataaccagc gatatgccat ttgaaggagc
     7981 aaccttattt attagaggag aaaattctga ttatatcact tttgaagatt ggcctcaaat
     8041 tcttcagctt tttccaaatg ccaatttatc taccgttaat gacgcaggtc attgggtgca
     8101 cgctgataat cctcagtctt taattaaaat cattcaggag tttttgtaat ataacttaca
     8161 aattatcatt aacgatatat gttaaatttg ataaatgtaa tttcgaatta atattgaaag
     8221 atttgttaac aaatcataac cgaagttgat tagttgtaaa tttgaataaa attacaatta
     8281 tgaaaaaact actactaatt acatttttat taacgccatt cgttgcgtta attgcgcaac
     8341 caacaattac cagcagtatt aatggtggaa ttggcgatga atttaattac atcgatgtac
     8401 aaacggaggg cttaaaccct ggtcctgcag gagaaaatgt tacatgggat ttcagcgata
     8461 taatagaatc aggagcagaa tacggttata cctgggttga tgttgcatca acacctgatg
     8521 gtggagattt tcccggcgca aatattgcag ccgataatgg tccaggtact tatagctatt
     8581 ttaaagttac cgcaagtgaa tttactaact acggagtatc agctgcgggt tcaattgttg
     8641 tttatcagga ccctgaagaa atttttgttt ttccgatgac atatggaaca actaacacgg
     8701 atgatttatt tagtgaattt atcaatggaa ttgcctttga aagaagtggc acagtaacta
     8761 tggaagtaga tgcatacgga acactgattt taccttcagg aacatatacc gatgtgttac
     8821 gtgtaaaagt aaaagaagat tacatggatg aggccgattt actcccaacg ccaattatat
     8881 acgattttac taattattat tggttaaaag caggtgtaaa aggtccttta ttccaatatt
     8941 ttgatttagt aaccgacgga ggatttccat ttgaagacca acaagcagca tatgctgaaa
     9001 atgttgctgt tggaattgct gaacataata ctattcaaag tttgacaatt ttcccgaacc
     9061 cggttaaaga agtggctact attattgttc ctgaaggaat taatagtgct gccatcagta
     9121 tttataacaa tttaggtcag gtggtaactt caacagttga aactgttaat cataatacaa
     9181 ttactttacc tgttggctat ttacaatccg gtatttatat gattgccctt actgaaggcg
     9241 ataataccta taatgctaaa atgattaaac gttaatattg aacgtatacc aaaactcata
     9301 ttgctgagta ttaagtaaca ataatactta taaaaaaaag cggctgttgt ttagatacaa
     9361 cagccgcttt gcttttttga taattggttt aatgctgtat tacaagtttt tcagttgttg
     9421 ttcctaatgt tccaactgtc tgcactaaat aaataccgga gttccattcc gaaatattta
     9481 attgtatcga ttcagctgct gcaatatttt ggttaaacat caatttacct gtcatatcga
     9541 aaatctgaat tgattgtata tcgctattta tcacaattgt taaattagtt gaagctggat
     9601 ttggatataa actaattatt gatccatcac cttccatatt atcacccgct ctgcaagcta
     9661 atgttgtaaa cgactgtaat ggcgaccagg cagaaatttc atctacgcca actgtatcac
     9721 aaattgttct tatttgccaa acataattgg ttccgcattg cagatttttt aaattgagtt
     9781 tattgttaac tcccggaaca aattttttct tccagtttat tgctgtagct tttttgtacc
     9841 aaacttcata ttgaaaagct cccggttcaa ctgtccagtt aagtcttgct ttaactgttg
     9901 taatattatt tgtaaataaa ccttcaggtg catcacaagt aataattgga tcttgaataa
     9961 aatgagcaag ggcaattcca ccaagcataa ataaactata atcgttgcca aattcgaaat
    10021 cggtattata accgctggat gctaatgcaa tccagtcgtg ggtgccatca gcatttactt
    10081 tacatactgc ctgttgaaat aaagtaccgg ctccaaaata aatattattg gcaatatcca
    10141 ttttcatgat gttgtgcaac aataaagctt gatcaggacc atcagcatct atgttagtcc
    10201 aaataggtgt tcctgtattt gtgtatttca tcatcgcaac accaaatgtt ccggcatttg
    10261 gaaaccctcc aattactggg taattatcag tcccaacttc tacttttgtg ccaaccattt
    10321 catttgtatg tgtccaggca atacttcctg caggtgaaag tttagtgagc acacttcctg
    10381 agcccccgaa ccaatcctga ttaatgatat aagtattacc atccaaatca cctgctgcat
    10441 caccaggtgt taaactgtat actcctgcaa cactccataa ttcatttcca tctaggtcaa
    10501 ttttagcata accatttaaa cttccaacag tacctcttcc tataatgaca attttattgt
    10561 ccggagtaaa tttaaaattc gtaggtgcac ctataccggc aacattaaag taattccaaa
    10621 tagctgttcc atcagccgcg aactttttaa ctttagtaac cattccaaca ccgttgctgc
    10681 caagtcccaa aacataaata ttattatccg catctactaa acatttcaca gtatatgaac
    10741 catcaaaact tgtttcataa acattgcgcc ataataaatt acctgaaggg tcaaatttca
    10801 ttactacact ggctgcattc accggcgaag catatcccga acgaatggtt ccggttacaa
    10861 taatattatt atcgttatct gtttctaccc atgtggcaac ttcatgacgg gtattatcag
    10921 tgttgtcata agaggtggtc catatataat ttcctgctgc atcgtgtttg gttaaataaa
    10981 tatccccagc aggattgtat tcataataaa tgctatacaa attatcggta tcatcggttg
    11041 caagcgaaag tccgccttca aattgttgcc agtcaagtgt tacctgcgcg tttattaaaa
    11101 acggcaataa caggaataaa aacatcgtct ttttcataat aagttgttta agtgtttgat
    11161 atttgttgct tagtatttta acgattaatt ggttattggt gttaattttt taaacgattt
    11221 aataaactga tttcccagcc ttcccaatta atctatgtat atacaaatag atagcttttt
    11281 tataatattt tgcgtgacct gtgtagatgc aaatggtgat tattatcact ttttcaataa
    11341 aaagaccgca atttatgttt gcggtctttt cgaattatgg gcattgaatg ctaatatttt
    11401 gtattaatta tgaataatta attgttgatt aatcacctca tcagttgttt cgatgcgaat
    11461 taaatacata ccggctgtta aactactggt attaatacct tcgcttggat catccgtagt
    11521 cgataataca acttgtcccg acatatcgaa gaaagttatt gattttaact cagcattttc
    11581 cgattgaata tatacgatat tgcttgcagg atttggataa atagaaacta ctgcagttgt
    11641 attcaattct ccttcacgca aaggaagtgt attgaaagtt tgttccggtg aaaaatctga
    11701 aactaatcca tccgcacaaa tagttctcac cttccaaacg tatgctttat ttggtgaaag
    11761 tgattttaca ttcaatttgt ttttggttga tggcacgaat ttcactttcc atgttggtgc
    11821 aatcgctttt ttataccaaa cttcatattg cactgcttcc ggaacaaaag accaattaaa
    11881 tcgagcctga ctgctggtaa tattattggt aaataatccc gttggtgttg agcacgaaac
    11941 agccataacc tgattgtaat gtaaaacaac tgcatattgt tggcccaaag caaagattga
    12001 attatctgac gctaaacata atgcggagcc tgtattggaa taatcggtat gcaaagcaac
    12061 ccattcttca acccctgctg gagtgtattt aatagttacc atttgcacta aactcaacca
    12121 aaaatatccc ggccatggac caccttgtcc tgttatataa atattatcat attcatcttt
    12181 aatcatcatt tttggtatct catcatttcc tgtgtgttca tcatacactt gtgaccataa
    12241 cataacacca ctgccatcta ctttaaaagt ataccagtcg aaatataaat cgccataacc
    12301 ggtaatgtaa gcaccacccg aattatcaca aactatttga cgtgaaaatt cttcatgacc
    12361 aaaatcgtaa ttattggtcc ataataatgt tccagacgaa ttaaatttac ttactacaaa
    12421 atctgatgaa ataaaaggag gtgttccaaa tgaaccgtgt gaagctgtaa aaatattccc
    12481 cagagggtcg atatcaaaac tcactaaacc atttgtaata ccacttgtat gccataacac
    12541 agttcctgca gtacttattt tagccaaact ggttccataa gcacctatca caatattacc
    12601 atccggtgca aattgcattt ttaacattgt gccaccaaaa tcatttccgg cagcatcttc
    12661 aataacagtc caaatatcat taccgtcagt atcatatttt tttaccatca tagtgccaac
    12721 ttcaccatgg taaatcatcc aggcattaac atcgccacca acataaatat taccttcagc
    12781 atcagtcaat aacttacgac cacggtatct aacatctgta gcatacgttt tgcgccagat
    12841 taatgaacca tctacaccgc taaatttcat cgtaaccatt tgtaaaggat accattcaga
    12901 accaaaacca acattggtat aaccggttac aatggcatct ccatttatat caatggcaac
    12961 atcacctgcc atttcccatt ccgaaggttc cgtattatca taagtggcag tccataaaat
    13021 ggtgcctgta gcattgcgtt tggtgagata aatatctccg ttaaatatgt cgcttaaagc
    13081 ataagtatta tcagcttcat caactacaat cgcacttccg tccaatccat agtaaaaatc
    13141 aggagaaacc cagtcttcag ttacctggga aaaaatcgtg cccaccattc ctaacagtga
    13201 cacaataaac agttgcattt ttatcataac agttcaaatt atatgtatat acaaatagat
    13261 agcttttata gcttgtagtg tgtgactaca ctcttgccat ctgttgactt atatcacctt
    13321 tttgcgggaa aatgacataa gtatataatt cgattgcggt aagaagaatg caaagataca
    13381 ttaatatagg gaaaaaaata acatgacttt tgtcacctta gtaataaatt ggcattttta
    13441 tccaattatt tcctaaatat tcactgatta tcaaacactt aggtggaatt tttttttgaa
    13501 catttttatt attccagttt gaaagtgcag tatttggtaa aaaaaaatga ccgacatagt
    13561 gccggtcatt tttggaaagt atatttttga tttaaatcaa agtgttcctc tcaattcctg
    13621 ttctctttca atggcttcaa acaatgcttt gaagttgcct ttgccgaaac ttcttgctcc
    13681 atggcgctga ataatttcat aaaacacagt cggacgatcc tctacaggtt tagtaaataa
    13741 ttgtaataaa tacccttcat catctctgtc gataaggata ttgagttttt tcaattcatc
    13801 aaaatcttca tcaatatgac caacgcggtc taatacatct tcgtaatata cttccggcac
    13861 atataaaaat tctacaccgc gtgctcgtaa ttcagtaact gtttcaataa tattatctgt
    13921 agcaatcgca atatgctgca cacctgcgcc tttataaaaa tcgatatatt cttctatttg
    13981 cgatttcttt ttcccttcag caggttcatt aattgggaat tttacataac cattgccatt
    14041 actaaccact ttactcatca atgcagaata ttccgtagaa atatcgctgt catcaaacgt
    14101 gataagtaat ttaaatccca ttacatcttc ataaaatttc acccactcat tcattttgcc
    14161 taattccaca ttccccacac aatggtctac atattttaaa cccacgggtt taaccggcac
    14221 attagatttt ttagtttgat atccgggcat aaatacaccc ttgtaatttt ttctttctac
    14281 aaatttgtga atagtatcac cataagtctg aatagccgat acaactactt caccaaattc
    14341 atcttttaac gtagttggcg caattacacc tttagcaccg cgtttaacag tttcttcata
    14401 actttttgtg gcatcgtcaa cccataaggc aagcacctta acaccatcac catgtttccg
    14461 cacatgttcc gaaatttcac tattcgggtc aaatgatgtg gttaatacaa ttcgtatttt
    14521 atcttgttgc aaaacatatg acgcactgtc cttaacaccg gtttctaatc cggcataagc
    14581 caccagttca tatccccatg cactttgata ataataagcc gactgcttag cattccctac
    14641 ataaaactca atgtggtcag tgccttgcag tggaaggaag tcggtggttg tttgggttga
    14701 agttaattct aaggtattca taaatttaaa atgtgctgat gtgctaatgt gataatgtgc
    14761 taattggcga agcttctgca agggcagaaa cagccgttaa cttttccatt ttagtcacac
    14821 ttagcaatca acgggtttat ttttttgatg atgaaataat tttacttaat attttattga
    14881 ttacaactat tctttccagt aaatcatcac attcagggta gttttttgca tgtttacata
    14941 atcgaagcca atattgtgac tcgctacctt cttttgcggc aattttcatt ttatggataa
    15001 aatccgccct gctctccgca ttttgtgatt catttacatt tgctccaaca gatgtacctg
    15061 catcaaataa ctgccaggcc attctgtttt tttttaattt ttctaactct tcggtgtaat
    15121 caataattaa taacgaaaat tcaaaagtta aatctacaat cagatttttt tcttcattac
    15181 tcattttttt tgttcttaag gtgataaaat atcttaaagt aataccaggg acaaagtgat
    15241 attactaaat tattcatttt aattaattca tactatcatt ttaattcatt ttgaaaatta
    15301 tcaatttatc aatttaccca atcagcacat tatcacatca gcacattagc acattaacta
    15361 agccaactca aatagtactc cgtatcctca atctccaaag cagcttcagt aagttgcaat
    15421 ggcctaaaag gatcaatcat aacagcaagt tctttggttt cttttttgcc aatgctgcct
    15481 tcgtatgtgc cggggtgtgg accgtgggga atgccgcccg ggtgaagggt aatttgaccg
    15541 cgttcaacac tttttcggct catgaaatcg ccgtcaacgt aatataaaat ttcatcgctg
    15601 tcaatattgc tgtgattgta aggtgcaggt attcctaatg gatgataatc atacatacgc
    15661 ggcacaaaac tgcacactac aaagttatgt gcttcaaaag tttggtgcac cggaggtggt
    15721 tggtgcacac gacctgtaat tggttcgaaa ttatgaatgg aaaaagccca tgggtataca
    15781 tacccatccc agccaattaa atcgaacggg tgattggcat aatgataagg ataaataata
    15841 ccctgctttt taattttaaa ataaaaatca ccctgctcgt cgtgagtttt taaattttgc
    15901 ggggttttga tatcgcgttc acaaaatggg gagtgttcca ttaattgccc aacacgattc
    15961 atataacgtt ttggaaattc tatcggtgaa aacgattcaa caattaataa cctgttgttt
    16021 tcatcattaa atgtaatttg atataatgtt cctcttggaa taatcacata atcaccatat
    16081 tcaaaaggaa tatctccgta catcgttttt aatgtaccgg ttccttcatg tacaaatatc
    16141 atctcatcag catccacatt tttataaaag tagtcggtca tacttttgcg cggagcagcc
    16201 aaagaaatat gtaaatcgct atttactaaa acagctttgc gactttccaa ataatcatct
    16261 accggtttta ttttaaatcc ttttagcgag cggtgtttta attgtttcga caacactacc
    16321 ttcggttcaa cactatacgc ttcaccaacc tgcttaattt ttgttggcgg atagaggtga
    16381 tataataaac tatacgtgtt gctaaatcct tctgttgaaa acagctcttc gtggtaaagt
    16441 gtcccatccg gcttgcggaa ttgaatatga cgtttctcgg gaatgttccc ggatttgtta
    16501 taaaatggca taaaatgtcc ctttttgagt atcctatagc aaaaatacaa aggaaatcat
    16561 tctaaaaaag gtgattttta tcggatacca tgatgttaac ttgcttacta gtttagacta
    16621 tacaacctct aatttagact ataaccacag ttaactcatg cattgatgga agttgtatgg
    16681 tttgttatcc tgacatttca ggatttttgg tgttgcagtt caggtttact taaacgattg
    16741 tgttggcaaa tacgctaaag tttgtagttc ataattaaaa tcaaccatca taacaggtat
    16801 atttgtcgat aataccataa tatcattttt ataacagccc aatttaacaa gatgaaatca
    16861 tattacatta ttgcaatttg cctgcttttt ttacaaagtt taatagttgt tgcacaagat
    16921 atacaagtga atacctcact gttggaggat aaattttcag ccgatgcatc aaaaaattta
    16981 ggtggtgacg aaaccgggtt ttatatcgcc agttttagtt ctaaatataa atttgaagtg
    17041 ggatttaatg ccacaggctc acaaacaaca ttgataattc aaaaatatag taacgacctt
    17101 aagctccttg cagaaaaagc aattcctcag gacgagaatg aaagtttaga atggccaatt
    17161 ctgtttaatg gcgatattta tagtatttct cgtaaataca gtaaaaaaac aagtagttgg
    17221 aatgtctatg cacaaaagtt ggaaccggta aaattatata attccggtgc ggctgcttta
    17281 ttatttaatg taaagcagga ggatgatcaa ctctatttgt cattggatat ggtaaaatcg
    17341 gcggacaaca aatttattgc tttccacgca tttccgatgc agtctatcgg cagagattat
    17401 ggagacaaat taggaaaccg attcaatagc aatatgacaa caatagctgt ttttggtgaa
    17461 ggtttggtaa agcagtggga aaaaaatgtg gtaataccct ctaccgacga taaatatacg
    17521 ccaactcagg tttcagtgga caataatggc gatgtgtata ttttaggatg gagatttatt
    17581 gaaggagcga aagcaactaa acctaataca attagagcaa taaccagttt gcatcaaatt
    17641 tcaaataaag gggctcagat gaaaaacata gatcttgttc aacaaggggt aaatatttta
    17701 gatgcattga tggcttttga taaagcaggc aatattatag ttgccggtgt ttgtagtgct
    17761 gctgatgaag ctgatgaaat ggatgctttt tatgctttta ctgtggataa atctgcatta
    17821 agtatagcta gtaagagtgt aaaaaatttc acgcaggatt ttctagataa aataaagctg
    17881 gataaggctt ttagagttaa attgtcactt gatgagttat tattaatgga taatggtgat
    17941 tttattttta tcattgaaga atttgctgtt tcaaatacac agtccggcac ctattttagt
    18001 tgcctgaatc tcattacact aggcgttgat tccaatgccg aaattgtttg gatgaatgca
    18061 atacctaaaa aacaattctc cattgaaaca gatttcacct cttataactt tatgcagaca
    18121 ggtgataaat tatatttatt ttataatggt aattccgaaa atttaaatgc caaaaattat
    18181 agagaattgg aagattataa aataaagaat tcacaaatta tgatggtaac tattaatagc
    18241 gatggctcct ataaaaaagt tgtgctgccg ggtgatataa gtgcttacca tttgcgcgtt
    18301 actatgtcgc aacaatccac agacaaaaaa tttattttct ttgcaagaga tggtaaaaaa
    18361 gaatatatcg gcgctattca ggtaaattaa ttattcagcg gttggtatcc atttaccggt
    18421 tataaacctc tgcgcttaat ttctttgtcg ataagattta attccctgct catttgtccg
    18481 ctcaccgatt tattttcctg agcacggcga attaaatagg gaattacatc tttaacggga
    18541 ccatagggca aatatttggt tacattaaaa ccggcttttg ctaaattgta agtgatttgg
    18601 tcgctcatgc catacaactg cgaaaaataa aaatgcgggt gatttgcagg aatatttctt
    18661 ttttgcattt ccgatgccag caataaacta cttttttcat tatgtgtagc agcacaaacg
    18721 gcaatgcggt ccatattttc gagacaaaat aaaattactg catcataatc gcggtctgtt
    18781 gcatttttat ccggttgaat aggtgaagga taattcattt cttcagcgcg tttgcgttct
    18841 ttatccatat acgcaccacg aaccggtttc aaacctaaaa tataattatg ttgtctggca
    18901 tcttccaatg cacgtttcga aaatgccaag cgatcgtgac gatacatttg tggtgtagca
    18961 aatacaactg ctttttgttt attgtatttg cgcattaatt caaacgttaa atcgtcgaca
    19021 gcatcctgaa tccaactttc ttctgcatca ataaataatc caatatggtt ttgtgcagct
    19081 gcggcagcaa tttggtccat acgggaatac aattttgctt tttctttttc ttcttctgct
    19141 gtgagtgttg tttttgaatt caatttttcc aataaagcaa aactggtaac gccggttact
    19201 ttactgctta taaacggaat atttttttta cctgaagcaa attgaataat acgaattaac
    19261 tcattttttg tccgttcaaa ttccgcttcc gattcttttc cctcaacacc ataatcgata
    19321 atagccccta tgtgaaattg ttctagttta tttaaaacag gaatggtttc ttccagggtt
    19381 tctccgccac aaaattgctg gtaaacagtg ccttttatgg cccatttaaa cggcaaatgt
    19441 aagttgagtg tccaactggc caaggtggtc agcgtatttg tcatcaccgg attattcatc
    19501 actttaaata accagtactc ccatttcagt tctttattgc ttttacccga aaaagctatt
    19561 tcggtatcgt taaaatcgat tttcggcgaa ggttccaagt tgtattgatt tttggcaaat
    19621 atacggggtt gttataaatg taaaaatgac tttgatgctt atatttgctc aaaatgatgg
    19681 atatgagagc atttggattg tttttaatgt tgggtttatc aatgcctgct attgctcagg
    19741 actacacgag ttactttacc ggcgacacta ctgatgtaac tgttacagtg caaaaaggta
    19801 cagttttaat gggtggggct tcggaaaacg acaatgccat acgctggttc ctcgaacgtg
    19861 caggaggtgg ggatattctg gtgttgagaa catcagggga agatggatac aacgactatt
    19921 tttttgcgga tttgggagtg gatgttaatt cagtagaaac aatagtatgt aataacaagg
    19981 atgcctcgtt taatccctat gtattgcgca gagtagcaga agccgatgca ttgtggtttg
    20041 caggtggtga tcaatgggaa tatatcgact attggcgtga tactcctatg gaggatgctt
    20101 ttaatctgct tattaatgaa aaacaaatta caattggcgg aacaagtgca ggttgtgcaa
    20161 ttatgggtca agcgtatttt tctgcagaaa atggaactat tatttctaat acagccttac
    20221 acgaccctta tcataattta ttgcaattag gaaatgacga ttttttgaat aatccggcta
    20281 cagcaaattt aattaccgat acacattatg ataatccgga tagaagaggt aggcaagtta
    20341 cttttttagc aagattaatg acggattttg gaatgccatt ttacggaatt ggggttgaag
    20401 aatatactgc agtttgtatc gacgaattta atattgcaca tgtatacggt tcgtatcccg
    20461 gtgatgatga tttcgcttat tttttgcagg taaattgtta taccccatcc ggtccggaag
    20521 tgtgtgctga tggtgatttg cttacatggg atagaaatga cgcagcaatt aaagtaatta
    20581 aaatggcagg cacgaattca ggaagtggga tggtagattt aaatgactgg aaaacagcat
    20641 caggcgcaga ttttacatgg gaaaactggt gggtagaaaa tgcagtgtta aaagtaacta
    20701 ctgatgcaac tgaaatagat tgtacaacac caattgcaca atccaataac ataaatactt
    20761 cgctagcgat aaatattgaa caagatgttt tacatattta tactgatatg ttactggaag
    20821 cgtataagct atatacaatt actggtcaat taattcaatc aggaaaattg ttaaataata
    20881 atatccaaat tcaacaattg aaaaatggta cttacctgtt ggttgtcatc gatgaacaac
    20941 aaaataccaa ttatattgaa ttcgtaaaat aaattgtgaa taggttacct tattaacgta
    21001 acataccctt ttttttcgtg aaaagtttca ctgatattgg tgcgatacca caaatgataa
    21061 atataaacac caatatcagc aggtttgcca tctacattgc cgtcccagcc ggttaaataa
    21121 tcatataccg acacaatttg tttgccgtat cgattataaa tttcgaaatt taatatcgtg
    21181 atattttcgt tggtgatgcg aaaattatca ttaataccat catgattggg agagaaggca
    21241 ttaggcatgt aaaaaacttc gggacaatct tctaaataat gtaattgaat aatggctgtg
    21301 tcagtcgcgc atccgttgtt tatagtaata gtataaattt tatcatgcac caataatcgc
    21361 tcagcaccta tatatccatc atcccaaaca aatatggggt tgctattgcc aggttcttca
    21421 atgagtttat gtaaattaat ttgcaccggt tgacctttac agactgctgt atcaaaatgt
    21481 acagtttgtg ttggcatttc aattaacgaa atattatcca ggtataaata ggcagcctga
    21541 aatacacccg gatctccact gcaaccgata aataaatcgc tgtaattatt tccgataact
    21601 ataaatcgtt cctcaccggt agcaataaaa caacaattaa ttttaaccca agcactagta
    21661 tcgttaaaaa tagtattttc aggattacaa aattgttgtt caattaaagt tgctgcagta
    21721 agaaatgagg ttgtagtata attaatcata ctatcactgg ttaaatacac acatatactc
    21781 gcaggaaaac aactactgat atcggcaaca cttgcatacc aaactaaatt ataagccgca
    21841 ttggcaacta atggtgatgt taattttgct tcaataaatt cgcgataagg tggcccaccg
    21901 gtttgtgcaa catacatacc acaataaccg cttccggact gagcttgttg aataccaaaa
    21961 taattggtag gaacagaagc tgtatcgttt atactgcatg tattaaaata ttcagatgca
    22021 gggtaagtcg cattgaacca tggctcaccg agtttaagtt ggcctaattt gttgggacag
    22081 gtatcgtatt cttcaaaatc gccattgggg ataaaatttt gacactttac ctcaattcca
    22141 cctataaaat gccatatcat caacaaaact gtgatgacag gtattttcga taaatagaaa
    22201 ttgggcactt taggtcgcat gtaatgccga tacttggaaa tccaaatgta tcccaaattt
    22261 aagtacgggt gccattttta gccttttttc aagtaaaatt tgtgtcagat tggggctttt
    22321 gaaactgacc aaatttacta tctttacccc gtgcagagtt ttgttgtttc agcgagaaaa
    22381 tacaggccct catcatggca tgatgttatt gggcaggatc aggtagtggt gacgttaaaa
    22441 aatgccatca aaaacaacca tttggcgcaa tcgtttttgt tttgcggtcc acgtggtgtt
    22501 ggtaaaacta cttgtgcccg tattctggcc aaaacaatta actgcgaaaa tattcaggaa
    22561 aacggcgaag cttgtaatac ctgtgaaagc tgttcttctt ttaatgaaaa tgcatcgttt
    22621 aatattcatg aaatagatgc agcttccaac aactctgttg aagatattcg tagtttagtc
    22681 gaccaggtgc gttatgctcc tgcttccgga aaatttaaaa tctatatcat agatgaggtg
    22741 catatgttga gtgcttcggc atttaatgca tttttgaaaa cactggaaga accaccttta
    22801 tatgtaaaat ttattcttgc cacaaccgaa aaacataaaa taattccaac tattttaagc
    22861 cgctgtcaga tttttgattt taatagaatc agaatcaagg atatgacaca acagcttgaa
    22921 ataatttgta aaaacgaagg aattgattgc acagatgaag ctatgcatct tattgctcaa
    22981 aaggcagatg gcgccatgcg tgatgcactc agtattttcg accgtatcac cagtttctcc
    23041 ggaaagtcta ttaccgctaa agatgtcatc gaaaatttaa atatcctcga ttatgaatac
    23101 ttttttaaaa taaccgatgc ctttgttgtt gaagacgtag caacggcatt agtgatattt
    23161 gatgaagtgg tgaataaagg atttgatggc gatattttta tcaacggatt agcagaacat
    23221 tttagaaaca ttttagtttg taaagatgaa gctacgttga aattattaga taccagtgaa
    23281 accgtaattg aacgatataa aacacaaagt aatttaatca gtgcttcgtt tttattaagc
    23341 ggattaaata ttttaaatag cacagatgtt aattatcgtg caagtaaaaa taaacgttta
    23401 cacgtagagt tggcattgat gaaattgtgt tatgtccaca gcgcagtttc tacgctttct
    23461 tcaggcagca tggaaaaaaa aactcctgac ctgaacgaaa agcgcccaaa tgctgccatt
    23521 aagcccattg cttcagcagt aaaaactact gaaaccaaca atatttccga catatcaacg
    23581 gtaattgaag agccagtaaa gaaggttaga gttgaaaata gcaccattat tacgccacaa
    23641 ttagaatctg ttgaaccagt tgttattcct aaaaaaaccg acattcgtca ggctattaaa
    23701 aaagaggaag acgaaaagtc cgcagcactt ttaaccaaaa ccgttgaaga gcctttaaag
    23761 gttatttctc ccgaaatgtt ttttatcgtt tggaaacaat attccgactt tatccgcaag
    23821 gaccaaaatc gtgtcgccat tattatggat gcggctgctg tttctattaa tgaaacaaat
    23881 aatatcgtag tgcgtttaga gtctaatctg gaagccaaca tcatgaagga aatgcaaaac
    23941 gacctgcgtg agtttattaa agacggactg gaaaataatc aattcgacat ttcgtttatt
    24001 g
//
LOCUS       DAORKW010000008        23318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_11_length_23318_cov_4.876800, whole genome shotgun sequence.
ACCESSION   DAORKW010000008 DAORKW010000000
VERSION     DAORKW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23318
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11_length_23318_cov_4.876800"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1895
                     /locus_tag="PLB46_00805"
     CDS             <1..1895
                     /locus_tag="PLB46_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HRF75078.1"
                     /translation="GLIPTLEAVFRKLDEPMPLGYCNLGIVTAVGEGVNDFKIGDRVI
                     SNGPHAEYVVVPKNLACKVPEDVSNEAAVFTVVGAIGLEAIRLAKPQLGDTVCVIGLG
                     LIGLLTCQLLRASGCNVMAYDIDDKKIAIAKSMQIAAFNSSNIDIDTHVLEQTNNFGC
                     DVVFIAASAKNDNLVNIAARATKKRGQVILIGVTNLQLDRTEFYKKEITFQVSCSYGA
                     GRYDDQYEIQGIDYPEAYVKWTAKRNFEAFLSCLNNNTVQTAQLITSTILFEQAPDAY
                     AEIATTDTVALLLKYANEDKVQSQPEGIAIKTNKPSAVTIGIIGSGNFAKATLVPILQ
                     KTGVNIKYICGNDGLSAALLAKKFNIPHAVTSADVIINDPEVNLLIIATRHDSHAALA
                     IAGLIANKHIFVEKPIAIDLNSLEQLKVAHALHPELIFQSGFNRRHAPLAKQLKAKIQ
                     NNPVSNIVITVNAGALPDGHWLYNKETGGGRIVGEACHFFDLMSYFTGSTIKSVYATE
                     APISNQQENTIVHLTYHNGSHGTINYFANGHKKYPKEKIEVFAGNKIFILDNFRKLYA
                     YGANISNQLFSKQDKGHKAQFENLIQSLHSDQNSSNTFESLYNTTKATLLVEKSITTK
                     APQEVD"
     gene            1899..3815
                     /gene="asnB"
                     /locus_tag="PLB46_00810"
     CDS             1899..3815
                     /gene="asnB"
                     /locus_tag="PLB46_00810"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRF75079.1"
                     /translation="MCGIAGLIKTSGNNSNFRDAFKNMQHRGPDATGIYEDDELILGN
                     HRLKIQDISDNGNQPFFDSGNRYAIVFNGEIYNHLDIRKKLSQYDIRYVSTSDTETLL
                     YGYIHFGEQILSALNGIFSFVIYDKVKKQLFGARDQIGVKPFYIYSKQNIFAFASELK
                     TLVALDNFDKTINYPALANYMNYLWSPGEATPFLYVNKLLPGECFTINLNEPGKLYRK
                     KYYEINFNGKRNNQDESAWTVAIDQQIKQSLKSQLISDVPVGFFLSGGIDSSLLVGQA
                     KSIMPNEPIHCFTIKNSNGNRQDGFEDDFKYSNEVAKSLGTKLHVVDGGIDVLHDFDK
                     MIWHLDEPQSDLAPIYVAKICEHAKQEGFKVLISGTGGDDVFSGYRSHQAIVTDKFTK
                     HIPNGVYQLASKIIGSGNANHPTVRRINKLIKHKNDDALTRLVGYFEWFPIMEHLDLF
                     HDDIQSQVVNNNPSDYMMELLVNVNNEHDLLNKMLYLELKTFLVDHNLNYTDKMGMSS
                     GVEVRVPYLDIDLIAMSTQIPTKLKVHNFQTKYILRRIAAQHLPQSIINRPKTGFGAP
                     IRNWVTNEMAAMVNERLSPSELNKYHIFNPVAVQQLFNDNKSGRIDGAYTIISIMAIQ
                     SWMSQFYDNDNLHN"
     gene            3852..5693
                     /locus_tag="PLB46_00815"
     CDS             3852..5693
                     /locus_tag="PLB46_00815"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRF75080.1"
                     /translation="MGIPYVIERSLYSIKQKSGLLKHTLTITPTHSFPITGSKWLNTR
                     GDFLFQDKTDLTLLKTPTATLKEKAAAIFSGKLPYFNYHYFEIPETNRWHIHPLTRYH
                     YPNNIYYFNINDFAEEQDIKYVWEASRFCYVYDIIRYDYHFQENQFDFIFKEITSWIQ
                     DNPLNYGPNYMSGQEVAIRLLNWCFCIHYYANEIANNEALWQKVMCSVYEQLKHIDAN
                     LFFSQKFVRNNHLISEATCLFVYSLLFPTLPESAKWQIKSKQILEQEAQFQIFNDGSY
                     LQYSMNYHRVIIQLYNWVLKIGNLNKVKFSDDFISQIKKTLQFLVQNTDPVSGYTPNY
                     GANDGSLIFPLNNNDYRDFRPQLQSLAHTLNLNVYSEVFEDTCWINNKCDFNFNETLF
                     QQKINQFNTGGYYNFSDAETFTFIRCGSHIRRPSQADNLHIDLWYQSQNIMRDGGTYV
                     YNGDAALREYFFGTQSHNTIKIGASNQMLKGSRFIWHFWSKANSAELIETDEAFIFNG
                     SINAFANKGNWATHTRRVVKPKKTASWVISDTVSNHNNLPITQIWNPGPMFEAQFNIH
                     AIDAHNNPITPVYEKGFYSNTYGQIETIQQILFHTTSNTIVTQITLK"
     gene            5698..6996
                     /locus_tag="PLB46_00820"
     CDS             5698..6996
                     /locus_tag="PLB46_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRF75081.1"
                     /translation="MHILLIHQYFQETDDPGGMRWNAMTKLWVEQGHTITVIAGMTHY
                     AKGTRNPKYNNKYVVVDEFAPGIKVIRTHVSRSYNNNFRGRMNAYFAFVFSGTIGGLL
                     KAKDKYDCIIVSSPPLTVGITALILKFFKRLPFVFEIRDLWPESAIDTGVLTNKFFIK
                     LALMLEKKLYKSATLINVLTPAMKNTLITKKGIAENKIIYFPNAADFELSEHLLINST
                     TNSLRRELDLPEDKLIVCYVGAHGVANHLIQVLDAAELLKNEPIIFLLLGDGMQKTNL
                     QEIASQRNLTNVIFRPAVPKADAMKYILASDIGMSILKKAETFKTVFSNKTFDYMACK
                     KPIILGIDGVSRELLATADAGIYVEPENPEALANAAVRYLHNRELISTQGENGYRFVK
                     ANFDRKILATNYIQTLSKFFDKKSAQTIMQKATTNVPQAD"
     gene            6977..7600
                     /locus_tag="PLB46_00825"
     CDS             6977..7600
                     /locus_tag="PLB46_00825"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRF75082.1"
                     /translation="MYRRLIKPLLDFLFAFIGIILCLPLFILISLYCAINFNGHVFFT
                     QQRIGKNEKTFTIYKFITMHPPTGNYKNKPDAERLTKAGALLRLTSLDELPQLFNIIS
                     GKMSWVGPRPLLPEYLPFYTNEEKKRHQVKPGITGLAQINGRNLVGWDERLQLDARYA
                     EKVSLLLDLQIVFRTAIYIIQRRDVVANPQSVLLPLHIARKLNSPNK"
     gene            7608..8741
                     /locus_tag="PLB46_00830"
     CDS             7608..8741
                     /locus_tag="PLB46_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF75083.1"
                     /translation="MENERIYLSPPHMGGTELAYIQHAFDTNWIAPLGPHVDAFENDL
                     SGYLHVKACAALSSGTAALHLALIQLNVQQNDYVICPTFTFSASANPILYQKAIPVFV
                     DSEKDTWNMDPELLEEAVQFCLRQNKKPKAIIVVHLYGMPAKLKAIQKIADDHGIPII
                     EDAAESLGSTYYGKHTGSFGTFGILSFNGNKIITTSGGGALVSSNIEAIKKTRFLATQ
                     ARDPAPHYQHSSIGYNYRMSNICAAIGRGQMEVLSNRIDARKKNFNYYHSRLSVLPAI
                     TFLPEPENVSSNRWLTTIQIDSTQTSATPNSIRLALEELNIESRPLWKPLHLQPVFAQ
                     FPSFVADTSANLFQNGLCLPSGSALTEIQLDRICSCVEAAIKA"
     gene            8878..10821
                     /locus_tag="PLB46_00835"
     CDS             8878..10821
                     /locus_tag="PLB46_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRF75084.1"
                     /translation="MSNNWSNYINREFGTRIISKWVVFIFDIVITLFTYGVAYTLRYN
                     FNTAAVSFDVFIDDIILTIVVYAISFLSFKSYDGIIRHSGVADALRLIKAGLMAIIIL
                     IVLTTISKAYDFSFGITPIAIAIIHLVINVSILVFSRYGIKVFFYEVNRNKISKIPVA
                     IYGAGRRGLSVFQAIKNDAESNFVVAAFLDDNDTKLNKTIEGVKIFSPKKLEAVINRY
                     QIQQLIIGIHEIEPQKKNDIVAICFEHQVSVKSVPPVANWINGQLSLKQLKNINIEDL
                     LGREPIMLENEQIHQTLSGKRILVTGAAGSIGSELVKQIIKYAPKQLILIDQAESPLF
                     DLKMDLLFAEQLRLHTQVAFIVCDITNQERLEKIFASHKPEMIFHAAAYKHVPLMEDN
                     VYEAVDVNIFGSKNLIDLAIKNNVEKFVMISTDKAVNPTNVMGATKRAAEVYVQYKSK
                     LFPSNTVFITTRFGNVLGSNGSVVNYFSKQIAQGGPLTITHPEVTRYFMTISEACQLV
                     LEAATMGKTSELYLFDMGKPVKIIDLARKMVLLSGLEPETDIPFVYTGLRPGEKLHEE
                     LLNTNENTLPTHHHKIKVALTAEYSPEIVESYIETLQNALKSGDTTDIVTALKHMIPE
                     FISQNSDFEHLDATKSDKLPVTN"
     gene            10917..11708
                     /locus_tag="PLB46_00840"
     CDS             10917..11708
                     /locus_tag="PLB46_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRF75085.1"
                     /translation="MLTKAHHRNKFVRILLMILIASPLFQACGIISPNKMLDADKGYT
                     GASFLNTNTPDFVIAQGDNVQVLVFTKNGYALIEPQISTQNNGNTPETNSVPLDYSID
                     INGEANLPLVGHVKLSGLTIREAETLLAQKYASNYIDPFVNVKITNKYITVYRGGTDA
                     RQVILSRPDMTILEAIGAAGGVPENGKASTITIVRKIDGQPQTEIIDLSEIESIAKAN
                     TNVLPNDVIYVEPAFNGSIIREVAPILTALSSIVLIYVSLVNLNR"
     gene            11719..14148
                     /locus_tag="PLB46_00845"
     CDS             11719..14148
                     /locus_tag="PLB46_00845"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRF75086.1"
                     /translation="MSNTGENLKNNEIAEEQSFNWKLFLTIGWITRWWVVLYIILAIV
                     ASFLISRYSTPLYLSQADVQFKDKSKENTIDLGILVSNTNVDRLNEEMTILKSKGYKL
                     DALKQLPIQVSYFIKENVKEGEIYKASPYEVKIDIIDSNIIGQKISVAIIDTNHYSLK
                     YLYDHEDLDFKFEFGKPYSTPAFNITARLVRRETPNLNKDYFFIINNVEKEAEKIEGK
                     IQIDVDNLYGGKIVINYRDNNPIKTRDIVNAIAEEIVAMSLKRKAGSAEMIITFIEDQ
                     IDSLEQELYNQENILKNFKKDNLIISPELAETNIIQKLNEIDQAKFEVLLEGKSLKWL
                     KDFTNNKNTDIDALANYFGDLQYNDFTPYLNSLVELNKERENLSMSVPSNDPRLSSIN
                     NQIKKVTANFKEAIVNAENKLEVRTKYLTEEQEKYEAEFLQLPEKQSEFARLTRLNDL
                     KEKYYLLLLEKQSEYEITLAGMSSDYVILDAGERGDLVAPVKSKIWGLAILICIGISF
                     AHIYLRFLLHSTIIGVPDVEKSTNVPLLGALPLYSKGKLDLPRVVVSESPKSQIAESF
                     RAIRSNMQYFIKNDNKSKLIVITSTVSGEGKTFTAINLANVISATGKKVILVDFDLRK
                     PKIHKAVDVKNIKGVSTILINRDTIAECILTDTNAGCDVLIAGPVPPNPAELLVSKAA
                     DKLLDDLLAIYEYVVIDSPPVGIVSDSIPIMAKADLAIYMIRVNYSKRNFIGNVHRLY
                     NDNQLTNLGIVVNDANASGTEYGYGYGYGYGYGYGYTGYNEYYSDSVGPTSFWKRFFT
                     INRKRKRKKTL"
     gene            complement(14143..17037)
                     /gene="uvrA"
                     /locus_tag="PLB46_00850"
     CDS             complement(14143..17037)
                     /gene="uvrA"
                     /locus_tag="PLB46_00850"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRF75087.1"
                     /translation="MKKTKSTNTVLPANLPEISDKDVTYLEVQGARVHNLKNINVNIP
                     RNSLVVITGVSGSGKSSLAFDTIYAEGQRRYMESFSAYARQFFGAIERPDVDNISGLS
                     PVISIEQKTTNKNPRSTVGTITEIYDFMRLLYARASDAYSYATGEKMVRFTEEQIIQS
                     IITKFNGKRIVLLAPVVRGRKGHYRELFEQIRKQGFLKVRIDGEIKDLIPKMQVDRYK
                     MHDIEIVIDRFKVDEDDLVRIKQSVAQALKSGKQFIMVMDADKGTVFNYSKKLMCLAS
                     GISYEEPSPNTFSFNSPYGACPKCKGLGVVYEVNLETLIPDKSKTINNGGIVALGEFK
                     DNILFKQIKSISTKYKFNLSTPIKDIPKKGVNAILFGNEGDAELPEDDSVFLGETDRW
                     YTLEFGGLTKLIKRCFSNSISENMRRWAEDFMEKATCDECNGTRLKKEALYFKVDEKN
                     IAELALMDIKELNNWFSDVDVRMNAKQQVIAKDILKEIRARIGFIQNVGLDYLSLDRP
                     TRTLSGGESQRIRLATQIGSQLVGITYILDEPSIGLHQRDNNKLIDALKDLRDSGNTV
                     LVVEHDKDMMMHADYVIDLGPGAGIHGGELVGEGDPVKFMQANTLTADYLNEQRKIEY
                     LKTRRKGNGKEITLQGATGHNLKDVAVKFPLGVLICVTGVSGSGKSSLINGTLFPIMQ
                     QHYYNSLKKPLPYKKIDGLKNIDKVIEIDQSPIGRTPRSNPVTYIDVFSEIRNLFAMV
                     PEAKIRGYKAGRFSFNVKGGRCDVCEGGGMKVIEMNFLPDVYVECEKCRGKRYNRETL
                     EIYFKGKNIADVLDMTVDHAADFFENHPRIFKKIKTLQEVGLGYIRLGQSSTTLSGGE
                     AQRVKLAEELSRKDTGNTFYILDEPTTGLHFEDIRVLMNVINDLVDKGNTVLIIEHNL
                     DVIKLADHIIDLGPEGGAGGGNILVEGTPEQVAKHTKSHTGKYLKIEMKL"
     gene            complement(17182..17601)
                     /locus_tag="PLB46_00855"
     CDS             complement(17182..17601)
                     /locus_tag="PLB46_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HRF75088.1"
                     /translation="MSDNIIHTYKAPEPVGLYPHARKVGNLLFLSGIGPREAGTNKIP
                     GVEIDKNGNFTAFDFEAQCRSVFHNVRTVLEASGARWEDLVDVTVFLTDMQRDFATYN
                     RLYAEYFKESQPCRTTVGITSLPTPIAIELKCIAALP"
     gene            17735..18898
                     /locus_tag="PLB46_00860"
     CDS             17735..18898
                     /locus_tag="PLB46_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75089.1"
                     /translation="MKSFIVTLLICNVLLFTNCDNTSTSTAPRATTLEDVSHAEPYAV
                     EETTTLPPTVTLNAPEAEKSTVSESIEARIDAEIKRQLKLNPELPALNEVAAAYNNIT
                     VKVFPGLADATGGETYMVDNAKFVVEAITDIIKTYMSNETDLVFLIDKTGSMSDDIAE
                     IKKSITAIIEQIAPFENTRVGFAFYGDKNIDKATWYSKVNLSDDFTAAKKIIASTHTT
                     GGGNLEESVYDGVAKTIKEMNWEEGRRRVILLIGDAPSLEPPYSEFTMEDVINMAKDE
                     SVSMNFYPIVIGLSGSINGVGKLAEVPTTDAEIITSIAPNPATNYTLLKATATGNYTV
                     EIFDINGKLIQTKKFNDNNTSIMTDELATGVYVIRLYDEDNKKIDSKKLVVRH"
     gene            complement(19019..20140)
                     /locus_tag="PLB46_00865"
     CDS             complement(19019..20140)
                     /locus_tag="PLB46_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRF75090.1"
                     /translation="MAYLKKIRRTPTTVLTTGFAAVAMMSACGTYSAAIQKENTNFQA
                     IVDTTSTPSTELLFIEDFYVVDANANAAPRLISDYTPEANLRGQSLITAHEQLPISEQ
                     LVLLGENIRLSEQAENENIGGVDYATPEEAEDFEVYDDLFMANELNAGEVFDPTKMTD
                     SIQVFLVSPWSNCYTFPLGKSKVNSDFGWRRSRFHSGIDLDLEVGDDVYASFDGIVKK
                     ADVVSGYGNLVVIKHFNGLETYYAHLSKILVAEGDTVDSGSKIALGGNTGRSTGPHLH
                     YELRYRGAAINPKYLIDFKNEDLTAEMFYLKKEHFKPSTTSSTSATSSTGKKYYTVKK
                     GDTLGKIAQRNHTTVAKICKLNGISSKKVLKPGQRLRIK"
     gene            complement(20152..21594)
                     /locus_tag="PLB46_00870"
     CDS             complement(20152..21594)
                     /locus_tag="PLB46_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HRF75091.1"
                     /translation="MFILSNYIGGKLQPPVSGNYLDNYNPATGKVYSKIPDSDENDVE
                     QAVNAAQQAFEAWSTMEPANRSRVLYRIAHLIEERFEALAEAESMDSGKPLALAKRMD
                     ITRARDNFEFFAASVLHFASESHHQKGKFINYTTRNPIGVVGCISPWNLPLYLFSWKI
                     APALAAGNCVVAKPSEITPYTAYLLSEICMDAGLPAGVLNIVHGTGPKTGASIVKHPK
                     IKGISFTGSTRAGKEIAAVAAPMFKKLSLELGGKNPVVIFADCDYEEMLNTTVRSSFT
                     NQGQICLCGSRIYIESSLYERFKADFVERAKKLVVGDPKLAETNVGAVVSEVHYNKIL
                     SYIDLAKEEGGTILCGGQAVKLSGELESGWYIAPTVIEGLTQQCRTVKEEIFGPVVTL
                     HPFNNEEEAVNLANQSDYGLAAVIWTNHLTRAHRLSETIESGIVWVNCWLERDLRTPF
                     GGVKQSGVGREGGVEALRFFTEPKNICIKL"
     gene            complement(21742..22383)
                     /locus_tag="PLB46_00875"
     CDS             complement(21742..22383)
                     /locus_tag="PLB46_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF75092.1"
                     /translation="MAHIVSFTFNPFYENTYIIYDETNECVVIDPGCYSKEEMEELGD
                     FITRKQLKPKYLINTHCHIDHVLGNKFVADTWRVPLLMHKGEIPVLHEVVNYAPTMGM
                     YYKGSPEPEIFVDEGDIVTFGNTSLKVFFTPGHSPASVCFYNEADKFIVSGDVLFRRS
                     IGRTDLPGGDYATLMQSIREKLMVLPDEIKVYAGHMQPTTIGEERSENPFLNE"
     gene            22473..>23318
                     /locus_tag="PLB46_00880"
     CDS             22473..>23318
                     /locus_tag="PLB46_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75093.1"
                     /translation="MKIKLHLNITYLALAIILFGTTVTFGQGTQVQFGQNRVQYKDFT
                     WQFYEGEHFLVYFSQGGQNLGKFTAQIAEADLIQIEDLLDYKLNSKPEIIVYNNIADY
                     NQSNFAIGKEEQYNLGGQTKIIGNKIFIYFDGNHQHLRKQVREGVGKVLLNSMIFGGN
                     LQEIVQNAVLLNLPEWFTEGLVSYIGEEWNSELDNQLREGVLSGRYQKFNKLTGEDAR
                     FAGHALWYYIAESYGEATIPNLLYLIRINRSLENGFLFVLGAGVNSTIDDWYKYLLAK
                     YQNEAK"
BASE COUNT         7647 a         4368 c         4137 g         7166 t
ORIGIN      
        1 atggattaat cccaactttg gaagcggtat tcagaaaatt agacgaacca atgccgttag
       61 ggtattgtaa ccttgggata gtaactgcag ttggagaagg ggtaaacgat tttaagattg
      121 gagacagggt aattagtaac ggaccgcatg cagaatatgt tgttgttcca aaaaatctcg
      181 cctgcaaagt cccggaggat gtaagcaatg aagctgctgt tttcacggtg gtcggagcaa
      241 ttggtttaga agctattcgg ttagcaaaac ctcaacttgg cgataccgtt tgcgtaattg
      301 gtttaggatt aattggatta cttacctgtc aattattacg cgcttcaggt tgtaacgtaa
      361 tggcttatga tattgatgat aaaaaaatag ctattgctaa atcgatgcaa attgctgcat
      421 ttaattcaag caacattgat atcgatacac atgttctcga acaaacaaac aattttggct
      481 gcgacgtagt atttattgca gcctctgcta aaaatgataa tcttgtaaat attgcagcca
      541 gggcgacaaa aaaacgtggc caggttattc tcattggtgt aactaatctt caattggaca
      601 gaactgagtt ttataaaaaa gaaataacat ttcaagtttc ttgttcgtat ggagcaggcc
      661 gctatgacga tcaatatgaa attcaaggta tagattatcc ggaagcatac gttaaatgga
      721 cagccaaaag aaattttgaa gcctttttaa gttgtttaaa caataacact gttcaaaccg
      781 cacaattaat tacatccaca attttatttg aacaggctcc ggatgcatat gccgaaattg
      841 caacaaccga tactgttgct ttattgttaa aatatgcaaa tgaggataag gttcaatcac
      901 aaccagaagg tattgcaatt aaaacaaata aaccttctgc ggttacaata ggtattattg
      961 gctcaggaaa ttttgcaaag gctactttag ttcctatact tcaaaaaaca ggtgttaaca
     1021 tcaaatatat ttgtggtaat gacggactaa gcgccgcttt attggctaaa aaattcaata
     1081 tcccacacgc agtaacatca gctgatgtta taattaacga tcctgaggta aacctattaa
     1141 taattgcgac gcgacatgac tcacatgccg cattagcaat tgcgggttta attgccaaca
     1201 aacatatttt tgtagaaaag ccgattgcca tagacctaaa ttcattagag caattaaaag
     1261 tagcacatgc tctgcaccct gaattaattt ttcaaagtgg atttaaccga aggcatgcac
     1321 ctttggctaa acaactaaaa gcaaaaattc aaaacaatcc ggttagcaat attgttatca
     1381 ccgtaaatgc cggtgcattg ccggatggac attggttata taataaagaa acaggtggcg
     1441 gacgtatagt tggagaagca tgtcattttt ttgacttaat gagttacttt accggtagca
     1501 ccataaagtc agtttatgcc acagaagctc caattagcaa tcagcaagaa aacaccattg
     1561 ttcatttaac ttatcacaat ggaagccatg gcactatcaa ttattttgca aacggccata
     1621 aaaaatatcc aaaagaaaaa atagaagtgt ttgcaggaaa taaaatattt attctggata
     1681 atttcagaaa actgtatgca tacggtgcca acatttccaa tcaattattt tcaaagcagg
     1741 ataaaggaca taaagcccaa tttgaaaact taatacaatc tttacattca gaccaaaact
     1801 cctcaaacac ctttgaatct ctctataata ctactaaagc tactttatta gtagaaaaaa
     1861 gcatcactac aaaagcacca caggaggtag attaacacat gtgtggaata gcaggtctca
     1921 ttaaaacatc cggtaacaat tcgaattttc gagatgcatt taaaaatatg caacatcgag
     1981 gtccggatgc tacaggcatt tacgaagatg atgagctaat tcttggcaac cacagactaa
     2041 agattcagga tatcagtgac aacggcaatc aaccattttt tgattccggt aacagatatg
     2101 cgattgtctt taatggtgaa atttataatc atctggatat cagaaaaaaa ttaagtcaat
     2161 acgatattcg gtatgtcagc acatccgaca cagaaacttt attgtatggg tatatccatt
     2221 ttggggagca aattttatca gcattaaatg gtattttttc ctttgtaatt tacgataaag
     2281 taaaaaaaca gttatttggt gccagagatc aaataggggt aaaaccgttt tacatctatt
     2341 ccaaacaaaa tatatttgct ttcgcatcag aattaaaaac gctggttgca ttagataatt
     2401 tcgacaaaac catcaattac cccgcgctgg ctaattatat gaattattta tggtctccgg
     2461 gtgaagcaac gccatttctt tatgtaaata aattattacc aggcgaatgt tttaccatta
     2521 accttaacga accgggtaaa ctgtatcgaa aaaaatatta tgaaattaat tttaatggta
     2581 aacgcaacaa tcaggatgaa tctgcttgga cagttgccat tgatcaacaa ataaaacaat
     2641 cactcaaatc acaactaatt tcggatgtac cggttggatt ttttctttcg ggaggaattg
     2701 attcttctct tcttgtaggt caggccaaat cgataatgcc aaacgagcct atacattgtt
     2761 ttacgataaa aaattccaat ggcaaccggc aggatggatt tgaagatgat tttaaatatt
     2821 caaatgaagt tgcaaaatca ctaggcacaa aattgcatgt tgttgatggc ggaattgatg
     2881 tattgcatga tttcgataag atgatatggc atttagatga accccaatct gatttagcgc
     2941 cgatttatgt ggctaaaatt tgcgagcatg ccaaacaaga gggttttaaa gtactaataa
     3001 gtggaacagg tggcgacgat gtattttccg gatatcgaag ccatcaggct atcgtgacag
     3061 acaagtttac caaacatatt ccaaacgggg tttatcaatt agcatctaaa ataattggtt
     3121 caggtaatgc taatcaccca acggtgcgca gaataaacaa acttattaaa cataaaaatg
     3181 atgatgcgct aacccgtttg gtggggtatt ttgagtggtt ccctatcatg gagcatctgg
     3241 atttatttca tgatgatatt caatcgcagg ttgtcaataa taatccgtcc gactatatga
     3301 tggaattgct ggtgaatgta aataatgaac acgatctatt gaataaaatg ttatatctgg
     3361 aattaaaaac ttttctagta gaccataacc ttaattatac cgataaaatg ggaatgtcgt
     3421 caggggtaga agtaagggtg ccctatttag atatagattt aatagctatg agtactcaaa
     3481 ttcctacaaa gctaaaagtt cataattttc aaactaaata tatattgaga cgtatagctg
     3541 cgcaacattt gccccaatcc attattaacc gcccaaaaac aggatttggt gctcctatac
     3601 gaaactgggt aactaatgaa atggctgcta tggtgaatga gcgattatcg ccaagcgaat
     3661 taaataaata tcatattttc aacccggttg ccgtacaaca actatttaac gataacaaat
     3721 caggccgcat tgatggcgct tataccataa tttcaattat ggctatccag agttggatgt
     3781 cacagtttta tgataacgac aatctacata attaattgag ttttcgcaga tatattgatt
     3841 acataaataa catgggtatc ccctatgtaa ttgaacgaag tctatatagt ataaaacaaa
     3901 aatccggact attaaaacac acattaacca taacacctac tcatagtttc ccaattacag
     3961 gaagcaaatg gctcaacacg agaggtgatt ttttatttca ggataaaaca gaccttaccc
     4021 ttttaaaaac accaactgca actttaaagg aaaaagcagc agccattttt tcaggcaaat
     4081 taccgtattt taattatcac tattttgaga taccggaaac aaacagatgg catattcatc
     4141 cgctaacgag atatcattac cccaacaaca tatattattt taatatcaat gattttgcag
     4201 aagaacaaga cattaaatat gtttgggagg cttcaaggtt ttgttatgtt tacgatatta
     4261 taaggtatga ttatcatttc caggaaaatc aatttgactt tatatttaaa gaaattactt
     4321 cgtggataca agacaatccg ctcaactatg gcccgaatta tatgtccggt caggaggttg
     4381 caattagatt attaaactgg tgcttttgta tccactatta tgcgaatgaa attgccaaca
     4441 atgaagcact ttggcaaaaa gtaatgtgtt cagtctatga acaactcaag catatagatg
     4501 ctaatttatt tttttcacaa aaatttgtcc ggaacaacca tctaatttca gaagccactt
     4561 gtttatttgt ttacagcttg ctgttcccta cattaccgga atctgcaaag tggcaaatca
     4621 agtcaaaaca aatattggag caggaagcac aatttcaaat atttaatgac ggcagctacc
     4681 tgcaatattc aatgaattac catcgtgtta tcattcagct ttacaattgg gtgctgaaaa
     4741 taggtaattt aaacaaggtg aaattcagtg atgattttat tagtcaaatt aaaaaaacat
     4801 tacaattttt agttcaaaac accgaccctg taagtggcta cacacccaat tacggcgcaa
     4861 acgacgggtc gttaattttt ccattaaata ataacgatta tcgtgatttc aggccgcaac
     4921 tacaatcgct ggcacacaca ttaaatttaa atgtgtattc ggaagttttt gaggatacct
     4981 gttggataaa taataaatgc gattttaact ttaacgaaac attatttcaa caaaaaatca
     5041 accagttcaa cacaggaggg tattataatt ttagtgatgc tgaaacattt acttttatta
     5101 gatgtggcag ccatattaga cgaccatcgc aggcagataa tttgcatatc gacctctggt
     5161 atcaatcgca aaacattatg agagatggag gaacctatgt ttataatggc gatgctgctt
     5221 tacgggaata tttttttggc acacaatcac ataatacaat aaaaattggt gctagtaatc
     5281 aaatgttaaa aggctcccgg tttatttggc atttttggtc aaaggcaaac tccgctgaat
     5341 taattgaaac agatgaggca tttattttta atggcagcat aaacgccttc gcgaacaaag
     5401 gaaattgggc aacacacaca cgtagggtag ttaagcccaa aaagacggct tcctgggtaa
     5461 tttcagatac tgtaagcaat cataacaatt tacccatcac gcaaatttgg aacccgggtc
     5521 ctatgtttga agcgcaattc aacattcatg caattgatgc gcataataat ccaataaccc
     5581 cggtttatga aaaaggattt tattcaaaca cttatggtca aattgaaaca atacagcaaa
     5641 tactatttca cacaactagt aatacgatag ttacccaaat tactttaaaa taacagcatg
     5701 catattttat tgatccatca atattttcag gaaaccgatg atccgggtgg tatgcgctgg
     5761 aatgctatga ctaaattatg ggttgaacag gggcatacca tcaccgtaat tgcaggaatg
     5821 acacattatg caaagggaac ccgaaatcct aaatacaata ataaatatgt ggtagtggac
     5881 gaattcgcac ctggcataaa ggtgataaga acacatgttt ctcgttcata taataataat
     5941 ttcaggggta ggatgaatgc ctattttgca tttgtatttt caggaacaat tggtggttta
     6001 ctcaaagcta aagataaata cgattgtatc attgttagtt cacctccatt aaccgtaggc
     6061 attactgcac taatattaaa atttttcaaa cgtttacctt ttgtttttga aattcgggat
     6121 ttatggcctg aatcagcaat agataccggg gtacttacca ataagttctt tataaagtta
     6181 gcattgatgc ttgaaaagaa attgtacaaa agtgctaccc ttataaatgt tttaacacct
     6241 gcaatgaaaa ataccttaat aacaaaaaaa ggtatagcag aaaataagat aatttatttc
     6301 ccaaatgctg cagatttcga attaagtgaa catctgctta ttaattccac aacaaattct
     6361 ctcagacgag aacttgatct ccctgaagac aaattaattg tttgttatgt aggagcacac
     6421 ggtgtagcta atcatttaat tcaggtatta gatgcagcag agctattaaa aaatgaaccc
     6481 attatatttt tattgttggg ggatggaatg caaaaaacaa accttcagga aattgccagt
     6541 cagcgcaatt taacaaatgt aatttttcga cctgcagttc cgaaagcaga tgccatgaaa
     6601 tatattttag ccagtgatat tggaatgtcc attctaaaaa aagccgaaac atttaaaacg
     6661 gttttctcca acaaaacatt tgattacatg gcctgcaaaa aaccgattat tttagggata
     6721 gatggtgttt ccagagaatt gctagccact gctgatgctg gtatatatgt agaacctgaa
     6781 aatcccgagg cgcttgcaaa tgcagcagta cgttatttac acaaccgtga attaatatca
     6841 acacaaggtg aaaacggata tcgttttgtt aaagctaatt ttgatagaaa gattttagcg
     6901 actaattaca ttcaaacgct aagtaaattc tttgacaaaa aatctgcaca aacaataatg
     6961 caaaaagcaa ctaccaatgt accgcaggct gattaaacct ttgcttgact ttttatttgc
     7021 ttttataggc atcattttat gtttgccttt atttatactt atcagcctgt attgtgccat
     7081 caattttaat gggcatgtgt tttttacgca acaacgcatt ggtaaaaacg aaaaaacgtt
     7141 taccatttat aaattcatta ccatgcatcc gccaactggt aactataaaa acaaaccgga
     7201 tgcagaaaga cttacaaaag caggcgcttt attgcgactt acctcattag atgaattacc
     7261 acaattgttt aatattattt ccggtaaaat gagttgggta gggcctcgac cattattgcc
     7321 tgagtatctg cctttttata ccaatgaaga aaagaaaaga catcaagtaa aacccggaat
     7381 aaccggcctg gcacaaataa atggccgcaa cttagtgggc tgggatgagc gcttacaact
     7441 cgatgcacga tatgctgaaa aagtgagttt actgctcgat ttacaaattg tgtttcgcac
     7501 agcaatttat attattcaac gccgtgatgt agttgctaac ccacaatcag tattgctacc
     7561 attgcacata gccagaaaat taaattcccc taacaaataa ttttattatg gaaaacgaac
     7621 gtatttattt atctccgcct catatgggag gtactgagtt agcctatatt cagcatgcat
     7681 ttgacaccaa ttggattgcg ccattaggcc cacatgttga tgcatttgaa aatgaccttt
     7741 caggatattt gcatgtaaag gcttgtgcag cattgtcatc aggaacagca gcattacacc
     7801 tggcattaat acaattaaat gtacaacaaa atgattatgt aatatgccca accttcacat
     7861 tttcagcctc ggccaatcct attttatatc aaaaagcaat tccggtattc gtcgatagtg
     7921 aaaaggatac atggaatatg gatcctgaat tattagaaga agctgtgcaa ttttgtttac
     7981 gtcaaaataa aaaacctaaa gcaataattg tagtgcattt atatggtatg cctgctaaat
     8041 taaaagctat tcaaaaaata gccgatgacc atggcattcc cataattgaa gatgctgctg
     8101 aatcgttagg ctcaacgtat tatggtaaac ataccggcag ctttggaact tttggtattt
     8161 tgtcgtttaa tgggaataaa attattacca cttcgggtgg tggcgcatta gtgtcatcga
     8221 atatagaagc cataaaaaaa acgcgtttcc ttgcaacaca agcgcgtgac ccggcacctc
     8281 attatcagca ttcttcaatt ggatacaatt atcgtatgag taatatttgt gcggctattg
     8341 gcagaggaca aatggaagtg ctttcaaata gaattgatgc ccgcaaaaag aattttaatt
     8401 attaccattc tcgtttatca gttttgcctg ccatcacatt tttacctgag ccggaaaatg
     8461 tttcatccaa ccgatggtta accaccatac aaatagatag cactcaaaca agtgcaacgc
     8521 caaactccat tcgacttgca cttgaggaat taaatattga atctcgaccg ctttggaaac
     8581 ctttacatct tcaacctgta ttcgcccaat ttcctagttt tgttgctgat accagcgcaa
     8641 atttgttcca aaacgggctt tgtctaccct ccggttcagc cttgacagaa atacaattag
     8701 accgcatctg tagctgtgtt gaagcagcaa taaaggctta aattttactt accttacact
     8761 aaagtttatt ttgtaattac aaaccagtaa ttttggcctt aacagccaac aattacattt
     8821 taccctatta ttgttatccc gaaccatctc cgtgaaccca aaagttgaaa cctatttatg
     8881 agtaacaatt ggagtaatta tatcaatcgg gaattcggca cacgcattat ttcaaaatgg
     8941 gtagtgttta tatttgatat tgttattacc ctttttactt atggtgttgc ttatacttta
     9001 agatataatt ttaatactgc tgcagtttcg tttgatgtat ttattgacga catcatttta
     9061 actattgtcg tttatgcaat aagcttttta tcttttaaat cttacgatgg tattattcgc
     9121 cattcaggtg ttgctgatgc gcttcgacta atcaaagccg ggttaatggc aataataata
     9181 cttattgtat taactacaat aagtaaagcc tatgacttca gtttcggcat aacaccaatt
     9241 gctattgcca tcatccatct ggtaattaat gtatctattt tagtttttag ccggtatggg
     9301 attaaagtat ttttttatga agtaaaccgt aataaaatta gcaaaattcc ggttgcaatt
     9361 tacggagctg gcagaagagg cttaagtgta tttcaggcca ttaaaaatga tgcggaatcc
     9421 aattttgtag ttgcagcttt tttagacgac aatgacacta agctgaataa aaccattgaa
     9481 ggtgttaaaa ttttttcccc taaaaaactg gaagcagtaa ttaacaggta tcaaatacaa
     9541 caacttatta ttggcattca cgaaattgaa ccgcagaaaa aaaatgatat agttgctatt
     9601 tgttttgaac atcaagtgag cgttaaatca gtgccaccgg tggctaactg gataaatggt
     9661 cagctcagtt taaagcaact aaaaaatatc aatattgaag acctgcttgg ccgtgaacca
     9721 attatgctgg aaaacgaaca aatacatcaa acactttccg gtaaacgtat tttggtaaca
     9781 ggcgccgccg gttcaatagg cagcgaactg gttaaacaaa taattaaata cgcaccaaaa
     9841 caacttatcc ttattgacca ggcagaaagt ccgttgtttg acttgaaaat ggatttatta
     9901 tttgcagagc aattgcgatt acatactcag gttgctttta ttgtttgcga tattactaat
     9961 caggaaagat tagagaaaat atttgcctca cataaaccgg agatgatttt tcatgcagca
    10021 gcttataaac atgttccgtt aatggaagat aatgtttacg aagcggtaga tgtaaatata
    10081 tttggctcaa aaaatttaat tgacctagcc ataaaaaata atgtagaaaa atttgtcatg
    10141 atttctacgg acaaagctgt taatccaact aatgtgatgg gagcaaccaa aagggcggca
    10201 gaagtgtatg tgcaatataa atcaaaatta tttccatcaa acaccgtgtt tattactacc
    10261 cgatttggca atgttttggg cagcaatgga tcggtagtaa attatttcag caaacaaatt
    10321 gcacagggtg ggccattaac tataacccat ccggaagtta cccgctactt tatgactatt
    10381 tcggaagctt gtcaactcgt tttggaagcg gcaactatgg gaaaaaccag tgaactttat
    10441 ctgtttgata tggggaaacc ggtaaaaatt atcgatttag cccgcaaaat ggtgcttttg
    10501 agcgggttag aaccggaaac cgatatccct tttgtgtata ctggtctccg tccgggtgaa
    10561 aaattgcatg aagaattgtt aaacacgaat gaaaatacct taccaaccca tcatcacaaa
    10621 atcaaggttg cgctaacggc tgagtatagc cccgaaattg tagaatcgta catcgaaact
    10681 ttacaaaatg cactgaaatc aggcgataca acggatattg tgaccgcctt gaagcacatg
    10741 attccggaat ttattagcca aaactccgat tttgaacacc tcgatgcaac taaatcggac
    10801 aaattgcctg tcaccaatta acttacattg ccagaattgg tgcttttgta atcaagccga
    10861 aaccctatat ttgtcggcta attatattta cattaaggta tagttaaagc aaattcatgt
    10921 taaccaaagc acaccatagg aataagtttg tcaggatact gctgatgatt ttgatagcat
    10981 cacctttgtt tcaggcatgt gggattatta gccctaacaa aatgctggac gcagataaag
    11041 gctatacagg agcaagtttc ctcaatacaa acacgcctga ttttgtaatt gcccaaggcg
    11101 ataatgttca ggttttggta tttactaaaa acggctatgc attaattgaa cctcaaatta
    11161 gtactcaaaa taacggcaac actcctgaaa caaatagtgt tcctcttgat tattccattg
    11221 atattaacgg agaagcaaat ttgccattgg ttggacatgt taaactatcg ggtttaacca
    11281 ttagggaagc tgaaacatta cttgctcaaa agtatgcatc caattatata gacccttttg
    11341 ttaatgtaaa aattactaat aaatatatta cggtatacag aggtggaact gacgccagac
    11401 aagtaattct ttctcgccct gatatgacca ttttagaagc aattggcgct gcaggcgggg
    11461 tgcctgaaaa tggaaaagca tcaactatca caatagttcg caaaattgat gggcaacctc
    11521 aaactgaaat aattgacctt tctgaaattg aaagtatcgc taaggcgaac acaaatgttt
    11581 tgcctaacga tgttatatat gttgaacctg catttaatgg ttccataatc agagaagttg
    11641 caccaatact tactgcatta tcaagtatcg ttcttattta tgtttcgtta gttaatttaa
    11701 atagataatt ttcagggcat gtcgaataca ggtgaaaatt taaagaataa tgaaatagca
    11761 gaggaacaat cgtttaactg gaaactattt cttacaattg gttggataac aagatggtgg
    11821 gttgtactgt atatcattct cgctattgtt gcttcctttt taattagccg atactcaact
    11881 cctttatatt tatcacaagc cgatgtgcag tttaaagata aatccaaaga aaacaccatc
    11941 gatttaggca ttttggtatc caacaccaac gttgatcgac tcaacgaaga aatgaccata
    12001 ctcaaatcga aggggtataa attagatgcg cttaaacaat tacctattca ggtttcttat
    12061 tttattaaag aaaacgtaaa agaaggtgaa atttacaagg caagccctta tgaagtcaaa
    12121 atagatatta ttgactccaa tattataggc caaaaaattt ctgttgccat aatcgacacc
    12181 aaccattata gtttaaagta cttatatgac catgaagatt tagattttaa atttgaattc
    12241 ggcaaaccct atagcacgcc ggcttttaac atcactgcaa gacttgtacg ccgtgaaacg
    12301 ccaaatctca ataaagatta tttcttcatc ataaataatg tagagaaaga ggctgaaaaa
    12361 attgaaggta aaatacaaat tgatgttgac aatttgtacg gaggtaaaat cgttatcaac
    12421 tatcgcgaca acaatccgat taaaaccagg gatatcgtta atgctattgc ggaagaaatt
    12481 gtagcaatga gcttaaaacg taaagccgga agtgctgaga tgataattac ctttatcgaa
    12541 gaccagattg actctcttga acaggaatta tataatcagg aaaatatttt gaaaaatttc
    12601 aaaaaagaca atctgattat tagccctgaa cttgccgaga caaatattat tcaaaaatta
    12661 aatgaaatag atcaggcaaa atttgaagta ttgctggaag gaaaaagttt gaaatggttg
    12721 aaagatttta ccaataataa aaatacggat attgatgcat tagccaatta ttttggcgac
    12781 ctacaataca acgactttac accctatttg aactcgttgg tggagctcaa taaagaacgt
    12841 gaaaatctca gcatgagtgt tccttcaaac gaccctcgtt taagttctat caataatcag
    12901 ataaaaaaag taactgcgaa ttttaaagaa gctattgtta atgctgaaaa caaattagaa
    12961 gttagaacaa agtatcttac tgaagaacag gaaaaatacg aggctgagtt tttgcaatta
    13021 cctgaaaaac aatctgaatt tgcacgctta acccggttaa atgatttaaa agaaaaatat
    13081 tatttattac tgctcgagaa acaatcagaa tatgaaatta cactcgccgg tatgagcagt
    13141 gattacgtaa ttttagatgc aggtgaacgc ggagatttgg tggctcctgt aaaatccaaa
    13201 atatggggtc ttgctatact tatttgtatt ggtatttcat ttgctcacat ttatttacgc
    13261 ttcctgttac atagcactat tattggtgtt cccgacgttg agaaatcgac aaacgtaccg
    13321 ctgctaggag cgctgccact atatagtaaa ggcaaattag atttacctcg tgtagttgtt
    13381 tcagaaagtc ctaaatcaca aattgctgaa tcatttaggg ccattcgaag caacatgcag
    13441 tattttatta aaaatgacaa caagtcgaaa ttaatcgtta ttacttctac agtgagtggt
    13501 gagggaaaaa cgtttactgc tattaacctg gccaatgtaa ttagcgcaac aggtaaaaaa
    13561 gtaatattgg tagattttga tttacgcaaa cccaaaattc acaaggctgt agatgttaaa
    13621 aacatcaaag gtgtaagcac cattctgata aacagagata caattgctga gtgtatttta
    13681 actgatacaa atgccggttg cgacgtttta attgcaggac ctgtaccacc aaatcctgca
    13741 gaattgttag tatcaaaagc tgctgataaa ttattggatg atttattggc tatttacgaa
    13801 tatgtggtaa ttgacagccc tcctgtcggt atagtttccg actctattcc tattatggcc
    13861 aaggctgatt tagcaattta catgattaga gttaattatt ctaaacgcaa tttcattggc
    13921 aatgtacaca gattatataa cgataatcag ttaaccaatt taggtattgt tgttaatgat
    13981 gccaatgcaa gcggaacgga atatggctat ggatatggtt atggctatgg ttacggttac
    14041 ggctacaccg gctacaatga atattactct gatagtgttg gacctacctc cttctggaaa
    14101 cgattcttta ctataaacag aaaacgtaaa aggaagaaaa cgttataatt tcatttcaat
    14161 tttgaggtat ttacctgtat ggctttttgt gtgtttagcc acttgttcag gtgtaccttc
    14221 aaccaggata tttccaccac cggcaccacc ttccggtcca aggtctatga tatggtctgc
    14281 gagttttatt acatcgagat tgtgctcaat aattaaaacc gtatttcctt tatcaaccaa
    14341 atcattaatt acattcatca atacacgtat gtcttcaaaa tgtaatcccg tggttggttc
    14401 atctaaaata taaaatgtat ttcccgtgtc tttccttgat aattcctcag cgagtttaac
    14461 ccgttgtgct tctcctccac tcaatgttgt tgacgattgc cctaatcgaa tataacccaa
    14521 tcctacttcc tgcaaagttt ttattttttt gaaaatcctt ggatggtttt caaaaaaatc
    14581 agccgcgtga tcaacggtca tatctaaaac atcggcaata ttttttcctt tgaaataaat
    14641 ttcaagtgtt tctctgttat atcttttacc gcggcacttt tcacattcta catagacatc
    14701 aggtaaaaaa ttcatttcta tcactttcat tcctccgcct tcacaaacat cacatctacc
    14761 acccttaaca ttaaatgaaa atctacctgc tttataacct cgaatttttg cttcgggtac
    14821 catggcaaat aagtttctga tttcagaaaa cacatctata taagtaacgg gatttgaacg
    14881 cggtgttctg ccaattggag attggtctat ttcaattacc ttatcaatgt ttttcagacc
    14941 gtcaattttt ttatacggca gcggtttttt cagcgagttg taataatgct gctgcatgat
    15001 agggaacaag gtgccattaa ttaaactcga ttttccacta ccactcacgc ctgttacaca
    15061 aatcaaaaca cccaatggaa attttacagc aacatctttt aaattatgtc ctgttgcccc
    15121 ttgtaatgtt atttctttgc catttccttt tcggcgtgtt tttagatatt caattttacg
    15181 ttgctcattt aaataatcag ctgttaaggt atttgcctgc ataaatttaa caggatctcc
    15241 ttctccaact aattctccac catgaattcc cgctcctggc cctaagtcga taacataatc
    15301 ggcatgcatc atcatgtcct tatcatgttc aactactaaa acggtgttac cactatcgcg
    15361 caaatctttt aatgcatcaa tcaatttatt attatcacgt tgatgcaatc cgatagaagg
    15421 ttcatccaga atataggtaa tgcccactaa ttgcgaacca atttgtgttg ccagcctaat
    15481 acgctgcgat tcaccaccac ttaatgttcg tgtaggtcgg tcaagcgaca aataatctaa
    15541 acccacattt tggataaacc caatacgtgc acgaatttct tttaatatat cttttgcaat
    15601 tacttgttgt ttagcattca tccttacatc aacatcacta aaccaattat tgagttcttt
    15661 gatgtccatt aaggctaact cagcaatatt tttttcatct accttaaaat acaatgcttc
    15721 ttttttgagg cgcgttccat tacattcatc acaggtagcc ttttccataa aatcttcagc
    15781 ccatctgcgc atattttcag aaatggaatt ggagaaacaa cgttttatca atttagttaa
    15841 accgccaaat tcgagtgtgt accacctatc tgtttcgcct aaaaataccg aatcatcttc
    15901 aggtaattcc gcatcgcctt cattaccaaa taaaatagca tttactcctt ttttaggaat
    15961 gtcttttatt ggagtggaaa gattaaattt atattttgta gagatggatt ttatctgctt
    16021 aaataaaata ttgtccttaa attctccaag tgctacaata ccaccattat taattgtctt
    16081 tgatttatcc ggaataagtg tttccaaatt tacttcatac acaacaccca accctttaca
    16141 ttttgggcat gcaccgtatg gcgaattaaa tgaaaatgta tttggagaag gctcttcata
    16201 agaaatacct gatgcaagac acattaattt tttggaataa ttaaatacag ttcctttatc
    16261 ggcatccatt accataataa actgcttccc cgactttaaa gcctgcgcaa cactttgttt
    16321 aatgcgtact aaatcatctt cgtcaacctt aaatctatca attacgattt cgatatcgtg
    16381 cattttatag cggtcaacct gcattttagg gattaaatct tttatctcac catcaatacg
    16441 cacttttaaa aatccctgct tgcgaatttg ttcaaacaat tcgcggtaat gaccttttct
    16501 tcctcttaca acaggtgcta ataaaacaat tcgcttccca ttaaacttgg taataatact
    16561 ttgtattatc tgctcttctg taaagcgcac cattttttca cctgtagcat aactataagc
    16621 atctgaagcc cttgcatata gcaggcgcat aaaatcatat atttcagtaa tagtacctac
    16681 tgttgaacga ggatttttgt tggtagtttt ttgttctatg gaaataaccg gactgagacc
    16741 ggaaatatta tctacatcgg gtcgttcaat ggcaccaaaa aattgtcttg cataggcaga
    16801 aaaactctcc atgtaacgac gttgcccttc tgcataaatg gtatcaaatg ccaacgaact
    16861 ttttccgctt ccacttacac cggtaattac caccaaacta tttctcggaa tatttacatt
    16921 gatatttttc aaattgtgca cacgagcgcc ctgcacttct aaataagtaa catctttatc
    16981 gcttatttct ggcaaattag caggtaaaac ggtatttgta gactttgttt ttttcacttt
    17041 ttgaaactcc ttacaatagc tcctttattt ctgattgcga actattttaa cgtgcaaagg
    17101 taagggtttt agtctcgaaa aacaggaaac ctccaataat agtagtagtg taaatggtaa
    17161 aatatttatc aacaaaagcc gtcagggcag cgctgctatg cattttaatt caatagctat
    17221 cggtgttggc aagcttgtta tgcctactgt tgttctgcaa ggctgacttt ctttgaaata
    17281 ttctgcataa agacggttat aagtagcgaa atcgcgctgc atatcagtta aaaaaacagt
    17341 cacatccact aaatcttccc aacgggcacc ggaggcttcc aaaacggttc ttacattatg
    17401 aaaaactgag cggcattgtg cttcaaaatc gaaagcagta aaattgccat ttttatcaat
    17461 ttccacgccg ggaattttgt ttgttcctgc ttcacgcgga ccaataccgg ataaaaacaa
    17521 caaattgcca actttacgtg catgtggata caaaccaaca ggttctggtg ctttataggt
    17581 atggatgata ttatcgctca tgtgccaaat ataaagtcaa aacaccaaaa ttgactactc
    17641 ttatactaat tttttgtatt taacgttttt taacccatgt gaagtgtgaa ataggtttga
    17701 tgtcagcatc ttgaatttac ctaaaaccaa ttatatgaaa agtttcattg ttacacttct
    17761 tatttgcaac gttttattat tcaccaattg cgacaacaca tctacatcaa cagcacctcg
    17821 tgccactaca ttggaggatg tatcccacgc agaaccttat gctgttgagg aaacaaccac
    17881 attacctcca actgttacac tcaatgctcc cgaagcggaa aaatccacag tgagtgaaag
    17941 cattgaggcg cgtattgatg ccgaaattaa aaggcaatta aaattaaacc ctgagttgcc
    18001 ggcacttaat gaagtggcag ccgcttacaa caacattacc gtaaaagtat ttcccggctt
    18061 agccgatgca acaggtggtg aaacgtatat ggttgacaat gccaaatttg ttgttgaagc
    18121 cattaccgat attattaaaa cctatatgtc gaatgaaacc gatttggttt ttttaattga
    18181 caaaacaggt agtatgagtg atgatattgc tgaaattaaa aagagtatta cggcaattat
    18241 agaacagatt gcccctttcg aaaataccag agtaggcttt gcattttatg gtgataaaaa
    18301 tattgataaa gcaacatggt atagcaaagt taatttaagt gatgatttca ccgctgcgaa
    18361 aaaaatcatt gcgtcgacgc ataccaccgg cggaggtaat cttgaagaaa gcgtatatga
    18421 tggcgttgct aaaaccatta aagaaatgaa ttgggaagaa gggagaagac gggtaatttt
    18481 attaattggc gatgcgccaa gtttagagcc accttatagt gaatttacca tggaagatgt
    18541 tattaatatg gcaaaggatg aatccgtttc tatgaatttt tatccaattg ttataggctt
    18601 atcgggaagt ataaatggtg taggcaaatt ggctgaagtg ccaaccactg acgcggaaat
    18661 tattacgtct attgcaccaa atccggcaac caattatacc ttattaaaag ctacggctac
    18721 aggcaattac acggtagaaa tttttgatat taatggcaaa ttaattcaaa caaaaaaatt
    18781 caacgacaac aatacatcta ttatgactga tgaattggca accggtgttt atgtaatccg
    18841 gttatacgat gaggacaata aaaaaatcga cagcaaaaaa cttgttgtac gacattaatt
    18901 acggttattt tttggggtta aatacagttg caaatcaccg atggaagtcg gtgatttttt
    18961 tgtaaaataa tgtacagaaa aaaggcactc gttttgagtg cctcgttata aatattattt
    19021 atttaattct taatcgttgc cccggtttca aaactttttt actggagatg ccatttaatt
    19081 tacaaatttt agcaaccgtt gtgtgattac gctgagcaat ttttccaagc gtatcacctt
    19141 tcttaacagt ataatatttt ttaccggtag atgatgttgc acttgttgaa gatgtagttg
    19201 acggtttaaa atgttccttt tttaagtaaa acatttccgc ggttaaatct tcatttttaa
    19261 aatcaattaa atatttagga ttaattgcag caccgcggta tcgcaactca taatgtaagt
    19321 gaggtcctgt gcttcgaccg gtgttaccgc ctaaagcaat tttacttcct gaatcaacag
    19381 tatcaccttc ggcaactaaa attttggata agtgggcata gtaagtttct aaaccattaa
    19441 aatgtttaat cactactaaa tttccgtaac ccgaaacaac atctgctttt ttcacaatac
    19501 catcaaaaga agcatataca tcatcaccta cttcaaggtc taaatctata cctgagtgaa
    19561 acctgcttct tctccaacca aaatcagagt taactttact ttttcctaat gggaaagtat
    19621 aacaattgct ccaaggacta actaaaaaca cctgaatact gtcggtcatt tttgtaggat
    19681 caaaaacttc cccggcattt aattcatttg ccataaataa gtcgtcatac acttcaaaat
    19741 cttccgcctc ttccggtgta gcataatcca caccaccaat attttcattt tcagcctgtt
    19801 cgcttaaacg aatattttcg ccaagtaaaa ccagctgttc actaatcggc aattgttcgt
    19861 gggcagttat aagagactgg ccgcgaagat ttgcttcggg tgtgtaatct gaaattaatc
    19921 ttggtgctgc attcgcatta gcgtcaacaa cataaaaatc ttctataaat agaagttcgg
    19981 ttgatggcgt cgaagtagta tcgacaattg cctgaaaatt tgtgttttct ttttgtatgg
    20041 cagctgaata agtcccgcag gcgctcatca ttgctaccgc cgcaaatccg gttgttaaaa
    20101 ctgttgtggg ggttcttctg atttttttta aataagccac tttcctccgt tttaaagttt
    20161 tatacaaata tttttaggtt cggtaaaaaa ccgaagagcc tctactcctc cttcgcgacc
    20221 tacaccggat tgtttaactc caccgaaagg cgtgcgcaaa tcgcgctcca gccaacagtt
    20281 tacccaaaca ataccgcttt caatcgtttc agacaagcga tgagctctag tcaaatgatt
    20341 ggtccaaatt acagcggcca gaccgtaatc ggattgattg gccaaattta cggcttcttc
    20401 ctcgttatta aaaggatgaa gagtaactac tggcccaaaa atttcttctt ttactgttct
    20461 gcactgttgc gtaaggcctt ctatcaccgt tggggcaatg taccagccac tttccagctc
    20521 cccagataat ttaaccgctt gtccgccgca taaaattgtt ccaccctctt ctttagctaa
    20581 gtcgatataa ctgagaattt tattgtaatg cacttccgaa actacagctc caacgttggt
    20641 ttccgccaat ttagggtctc caactaccag ttttttcgct ctttcaacaa aatctgcctt
    20701 aaaacgctcg tataaactgc tttctatata aatcctgctt ccgcacaaac aaatttgccc
    20761 ttgattggtg aaagagctgc gaacagtggt gttcagcatc tcctcataat cgcaatccgc
    20821 aaaaattacc acagggtttt taccacccaa ctctaagctc agttttttaa acattggcgc
    20881 tgcaacagcc gcaatttctt tacctgctct ggtgctgcct gtgaatgaaa ttcctttaat
    20941 tttcgggtgt tttacaattg aagcccctgt ttttgggcct gtaccatgaa caatgttcag
    21001 cacgcctgca ggtaacccgg catccatgca tatttcggat aataaataag cggtgtaagg
    21061 agtaatttca gatggtttag ctaccacaca attgccagct gctaatgcag gagcaatttt
    21121 ccaggaaaat aaatataatg gcaagttcca gggagatata catccaacaa caccaatcgg
    21181 attgcgggtg gtatagttaa tgaattttcc tttttggtga tggctttcgc tggcaaaatg
    21241 taataccgaa gcagcaaaaa attcaaaatt atcgcgcgca cgggttatgt ccattcgttt
    21301 tgccagggca agaggtttcc ctgagtccat tgattccgct tctgccaaag cttcaaacct
    21361 ttcttcaatc aaatgtgcaa tgcgatataa aaccctgctt ctgttagccg gctccatggt
    21421 agaccatgct tcaaatgctt gctgtgcggc gtttacagcc tgttcaacgt cattttcatc
    21481 agaatcggga attttcgaat atactttacc tgttgccgga ttgtagttat ccagataatt
    21541 accgcttaca ggcggttgaa gcttgcctcc aatatagttt gataatatga acaaatcgta
    21601 tccgtaaagt tttcgaattg attaacggtt gcgaagatac caatcggagg gcaaattgtt
    21661 aaataataat cgaaatttag acccaaaaga cgttaaaaac gcagctttta ctggacaatc
    21721 ccgctgaaac agcacctgac attattcatt caaaaacggg ttttcactgc gctcttcacc
    21781 aatggttgtt ggctgcatgt gaccggcata cacttttatt tcgtcgggta ataccataag
    21841 tttttcccgt atcgattgca tcaatgtggc ataatcgcct cccggcaaat cggtgcgacc
    21901 aattgaacgg cgaaaaagca catctccact cacaataaat ttatctgcct cattataaaa
    21961 acaaacactg gccggagaat gcccgggggt gaaaaacact tttagactgg tgtttccaaa
    22021 tgttacaatg tcaccttcat caacaaaaat ttcaggttcg ggtgaacctt tgtaatacat
    22081 acccatggtt ggtgcatagt tgactacttc atgcaaaacc ggtatttccc ctttgtgcat
    22141 caaaagcggg acccgccagg tatcagcgac aaatttgttt cccaacacat ggtcaatatg
    22201 acaatgggta ttaatcaaat atttgggttt caattgcttg cgcgtaatga aatcacccaa
    22261 ttcctccatc tcctcctttg aataacaacc ggggtcaatc acaacacatt cgtttgtttc
    22321 atcgtatata atgtaggtgt tttcataaaa ggggttaaac gtaaaactta caatatgggc
    22381 catctatata tcgttatttt gtaaaatttt tcagaataat tgaactttac ggcaaaacta
    22441 cgtattttgc aatggtgatg agggctctag gtatgaagat taaattacat ctcaatataa
    22501 catatttagc attagcgata atactatttg gcaccactgt aacctttggc cagggaacgc
    22561 aagttcaatt tggtcaaaac agggttcaat acaaggattt tacatggcaa ttttatgaag
    22621 gcgaacattt tttagtttac ttcagccagg gtggtcaaaa cctgggcaaa tttaccgcac
    22681 aaattgccga agccgattta attcaaattg aagatttatt agattacaaa ctcaattcaa
    22741 aacccgaaat tattgtttac aacaatattg ctgattacaa ccaaagtaat tttgcaatag
    22801 ggaaagaaga acaatataat ctcggtgggc agactaaaat aattggcaat aaaatattta
    22861 tttattttga tggcaatcac caacatttac gtaaacaagt tagagaaggt gttggcaagg
    22921 tattattaaa ctccatgatt tttggtggca acttacagga gatcgttcaa aatgctgtat
    22981 tattaaattt acctgaatgg tttaccgaag gtttggtttc ttatattggt gaagaatgga
    23041 atagtgaact cgataatcaa ttgcgagaag gtgtattatc cggtcgctac cagaaattta
    23101 ataaactcac cggcgaagac gcgcgatttg ctggccatgc attgtggtat tatattgctg
    23161 aaagttatgg cgaagcaacc attccaaatt tattgtattt aatacgcatt aaccgttcgc
    23221 ttgaaaatgg atttcttttt gttttaggag ccggagtaaa cagtacaata gacgattggt
    23281 ataaatattt attagcaaaa tatcaaaacg aagcaaaa
//
LOCUS       DAORKW010000009        23124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_14_length_23124_cov_5.415016, whole genome shotgun sequence.
ACCESSION   DAORKW010000009 DAORKW010000000
VERSION     DAORKW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23124
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14_length_23124_cov_5.415016"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PLB46_00885"
     CDS             <1..1028
                     /locus_tag="PLB46_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75094.1"
                     /translation="YTFVIPVFDDIIDESDETLILDLTYISGACAVGLPSSFTLLITD
                     NDDTPLPVYTEYDIAEVTTEDADGIALSIDEKVDLTGIVYGINTWDGGLQFTLIDNTG
                     GISVFSFDNTFGYTVTEGDEVNILGTISQFNGLTEVEPDTLIFIDGGNTLKTPTEITV
                     LNESTESDLVSIENGLTYVDITQWLGDGSSFNVDLTDGTNTYTIRIDDANELSTMPAP
                     SIVEPFVLMVTGLGNQFDSDAPYTSGYQLFPRYADDIELISVLEVEDATQSGIQIYPN
                     PVTDYFIVEGQQNIISVELVNQFGQVILLENNQSNTYSLNNIAAGIYSVKITTTNGVV
                     SDNIVKL"
     gene            1170..2153
                     /locus_tag="PLB46_00890"
     CDS             1170..2153
                     /locus_tag="PLB46_00890"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HRF75095.1"
                     /translation="MSKLSANVKARFHHEMYHGWFKKRRFFEGWYFKIVDKSEQHALA
                     IIPGIAMDESGEKQSFIQVLDGVNVTAAYHKFDAIDFRSNENRFKVRIRENSFSTNRI
                     KLDIPGIKGLLHFDEHFFWPKKIISPGIMGWYSWVPFMECYHQVVSMNCAVTGKLIIN
                     KNEVDFVNGRAYIEKDWGRSFPSSWIWMQSNHFTKQPVSFKLSVAKIPWLSGAFVGFI
                     CAFLIDNKLIRFATYTGAKLKSISAEGETIDIVLEDAKYVLTVQAQKAAGAELASPVL
                     GLMEGRVKESMRALIHITLFSKQENKVIFNDTGRNAGLEIAGNTTELLNFK"
     gene            2158..2706
                     /locus_tag="PLB46_00895"
     CDS             2158..2706
                     /locus_tag="PLB46_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75096.1"
                     /translation="MRNLLFLIFFIPILVLAQDTLPYSEFRFTIVEVNGNDLTDDKIE
                     ARIAEVFYTDTSKHDLDYVFTQLAYSKKDSCWILKQNEPLGYNYKIEIFRKSDTVNPR
                     LLELRKMTLIYNGYDPENKSGCQYCICNEIPMEKGVFVIDVPRKMESWAYIRKVAINV
                     KNVPTEFRDITAIQNWFFRKNK"
     gene            2727..3170
                     /locus_tag="PLB46_00900"
     CDS             2727..3170
                     /locus_tag="PLB46_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRF75097.1"
                     /translation="MENLQPYYDLVESVIESFGINATECRGEKSGMWKMYKGSAEVWI
                     DVWYIEKEQRSYFQVMAPVMPVPAQNREGFYLELLELSDGMFDVAFSIHKDWAYIKNI
                     REAAGIDFTEIRSTINRVGSYCDEHDDRLKAKYGPPQAPGAPPTK"
     gene            3301..4458
                     /locus_tag="PLB46_00905"
     CDS             3301..4458
                     /locus_tag="PLB46_00905"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HRF75098.1"
                     /translation="MPVFIGIAAILTLFSCKPTEPDTTSLKKEIIKTYSQVVFATYED
                     ALTTAKLLQSAVDTFLINPTELHLKAAQKAWITARAFYGQTEVFRFYDGPIDNGETGV
                     EGLLNAWPLDEVYVDYVVGEPNAGIINNVQEYPAITRDLLVSLNEKGGEENISCGYHA
                     IEFMLWGQDVSDKKPGQRPYTDFIPGTVSNDRRRTFLQLNTGLLIEHLTKLCDAWQPG
                     KTDNYAAKFLSVNPDEALQKILTGMGVLSKVEMAGERMFTAYDNASQEDEQSCFSDNT
                     KADLQNNIGGIINVFYGNYVRYDKTNVDGAGIDDLLKNNHSAMLDSTVLMLKKCNNDI
                     QNIYYPFDQAIVVENKREQVMKAILTTQQLGEQFSIIAATLGITINVNAAD"
     gene            4458..5894
                     /locus_tag="PLB46_00910"
     CDS             4458..5894
                     /locus_tag="PLB46_00910"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HRF75099.1"
                     /translation="MKFNSNKITIFTITAVMVLFIGISFFACENFVPNNAATALVNQP
                     NETLSGGAATVFDQSVNAFALPIPTLSSQDELLFFVGNSFFNQNWVQAPSSTTARDGL
                     GPYFNAKSCSGCHFKDGRGQPPASIGQLSEGFLLRLSVPGTDKNGGPVGDKNYGLQFQ
                     DHAIDGLLAEGIFQLQWEEISGKYPDGTTYHLRKPKIIFDELNYGEMSSDILISPRVA
                     PQMIGLGLLEAIDEKTILAFVDEQDKNNDGISGRANYVWNHITQQQQIGRFGWKANEP
                     TILQQISGAFNGDMGIRTQYFKDQHILPAQKKAATLPNGGETEIDNDDLEKLHLYSAT
                     LAVPARRDVNDKNVIAGKNIFMALKCNACHIDKMVTGTHPKFTVLSNQTIQPFTDLLL
                     HDMGPGLADNRPDFLASGNEWRTPPLWGIGLFKTVNKHTFYLHDGRARNLEEAILWHD
                     GEAANAKNGFMQLKESERKQLLQFLLSL"
     gene            5987..6679
                     /locus_tag="PLB46_00915"
     CDS             5987..6679
                     /locus_tag="PLB46_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRF75100.1"
                     /translation="MPAPTNAVSSPVAINKLSTPEFLVGKHNYYTDTNFVEIPAHMAY
                     RTEMFLLDEVYAAYFEMYTAAKKEGVNLKIISASRTFEEQKWIWEDKWKKNKATYPAD
                     TSLAKFIMQYSAMPGTSRHHWGTEVDLNSTSDSYFATAAGAKVYKWLTDHAAEYGFCQ
                     TYDSKGISRHEGYNEEKWHWSYFPIADPLLKKYEELVEYSHIQGFSGDNAAPKLDVIN
                     NYVLSISHECEK"
     gene            6827..7198
                     /locus_tag="PLB46_00920"
     CDS             6827..7198
                     /locus_tag="PLB46_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRF75101.1"
                     /translation="MNIYVSGLSFKLNDADLRQLFEEYGEVSSAKIIMDKFTGRSRGF
                     GFVEMNDEDGQKAIDALNEAEVDGKVIGVSVARPREERPRNNFNRGGGGGGGFNKGGG
                     GGGRNNDRDGGGYGKKNFNRW"
     gene            7313..9523
                     /locus_tag="PLB46_00925"
     CDS             7313..9523
                     /locus_tag="PLB46_00925"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRF75102.1"
                     /translation="MRYTLTSFLVIITAISFAQSFYQLENTDIPDDGTSIMFEMNVSG
                     LPSTIDGDFGLESVCFNINHTWDSDIDVLLIAPDGSSFVLVSGVGWAAVNFENTCFNS
                     FADNFISDGVGPFTGEWKPIGDMGAVNNGQNPNGIWKLYVYDTWAFADAGFMIDWSIT
                     FGDNPAIPFPFYSSNLPIVKINTLGAIMNEPKTEASLYIIDGGIGAINIPSDTNYAFE
                     GKILVELQGFSGPYYPKKNYDFDIVDDAGLEYDTVLLGLPSENDFILKAEYLDYSLMK
                     NAITYEMYRKMGRYAPRTKYCEVMVNGEYKGVYSLTEKIKRDENRVDIAQLNPEDISG
                     EELTGGYIFEINENYTPNDWESDYLPINYASCGLPVAYKMVYPRVEEIQPEQLDYIVA
                     YVDSFEDALYGDEFLDEANGYRNFISVKSFVDFLITNEFSANYDSYGRSTFLHKDKNG
                     EINIGPAWDYDRAFAPWDVSGWVWENTHPAWPFPFWWSRLQDDEEWRNEVYCRYNDVR
                     QHILTDENVTAFIDSVDAYIGDAAARNFLKWQELGVGDPEYFVTELRDFIDARLNWMD
                     ITLAADFVPAPDASFTSTSLGALTYAFTPVTTNADYLWDFGDGIQSTEENPIHTFSGA
                     GDYLVSLTVNKYYGCIGNSDEMFQANVSIADMGLNGITIFPNPATDFIQVESNNNVVI
                     NETALFTVEGKYIETKISTTANGIVIDLKNVAAGTYLLKIKSGETVINYPFIKY"
     gene            complement(9561..10031)
                     /locus_tag="PLB46_00930"
     CDS             complement(9561..10031)
                     /locus_tag="PLB46_00930"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF75103.1"
                     /translation="MQLNQYLTDTFLFNDKANKMVLEKMQQMPDPSECIKHFSHLINC
                     QFKWMDRIRIYPATSTLDWWNPVYPMDELASYWDKSLQPWLDLLAEKSEAELLENVVW
                     IGVGNKPYTSPLKDLALQLNYHSIHHRAQMQLFIRQQGLTPDFVDYIGTIYKPI"
     gene            complement(10299..10859)
                     /locus_tag="PLB46_00935"
     CDS             complement(10299..10859)
                     /locus_tag="PLB46_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF75104.1"
                     /translation="MKIYVKNMACESCKVVVKEALEELDIKPVKVELGEIETKEEISD
                     EDKLKLNKKIKKVGLELLEKKQGVLVEKIRQVIIDYVYNTEEKPTMKFSALLSKELNH
                     SYTYLANFFSEVEATTIEQYMIGLKIERIKELIIFGEHTLSEIAYMLHYSSVAHLSAQ
                     FKKATGLTPTHFKQLKEKRRIAIQNI"
     gene            complement(11231..11659)
                     /locus_tag="PLB46_00940"
     CDS             complement(11231..11659)
                     /locus_tag="PLB46_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HRF75105.1"
                     /translation="MYPKELVQPMKEELTKVGFEDLTTPEMVQETIKGTEGTLLLVIN
                     SVCGCAAGNARPGVRLSLSNENKPDRLATVFAGFDLDAVAEARKLTLPYPPSSPSIAL
                     FKSGKLVYFIERHQIEGRNAMMIANDLANAYNKYCGVEAN"
     gene            11838..13154
                     /gene="ahcY"
                     /locus_tag="PLB46_00945"
     CDS             11838..13154
                     /gene="ahcY"
                     /locus_tag="PLB46_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRF75106.1"
                     /translation="MNVATDKKLPYKVKDITLAAWGRKEIKLAEAEMPGLMSLREEYG
                     NSKPLKGARIAGCLHMTIQTAVLIETLKELGADVQWSSCNIFSTQDHAAAAIAAAGIP
                     VYAWKGETLEEYDWCIEQTLFFGENNEPLNMILDDGGDLTNLVLDKYPEMVKHLKGIS
                     EETTTGVHRLYDRVVKGTLPVPAINVNDSVTKSKFDNKYGCKESLVDAIRRATDIMMA
                     GKVAVVAGYGDVGKGSAQSLRGSGARVLITEIDPICALQAAMDGYEVKKMDDAVKEAD
                     IIVTATGNCDIITGRHFENMKHNAIVCNIGHFDDEIDVAWLNNNFGHTKDEIKPQVDK
                     YTVNGKDLILLAEGRLVNLGCAMGHPSFVMSNSFTNQTLAQLELWLNGGNYKNKVYTL
                     PKHLDEKVARLHLGKLGVELEILSDKQASYIGVEKSGPYKPEYYRY"
     gene            13322..14035
                     /locus_tag="PLB46_00950"
     CDS             13322..14035
                     /locus_tag="PLB46_00950"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRF75107.1"
                     /translation="MTKLSVNINKIATLRNARGGNVPNVVQFAKDCERFGADGITVHP
                     RPDERHITLKDVYDLAPVVKTEFNIEGYPDARFMEIIRTIKPAQATLVPDPPNVITSN
                     TGWDVMANKQFLIDVVHEIQSYGVRVSIFLNPEIHQVHEAKLTGTNRIEFYTGPYAEH
                     YFKDKHSAVALYADAAKAAYEIGLGINAGHDLSLQNLQFFKQSLLHIDEVSIGHAIVS
                     DALYYGIENVIQMYKRLLQ"
     gene            complement(14123..14965)
                     /locus_tag="PLB46_00955"
     CDS             complement(14123..14965)
                     /locus_tag="PLB46_00955"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRF75108.1"
                     /translation="MKKLLIVAVAVTSALAFSSCAKDRIYGCTDPFSISYNPNATDDD
                     GTCFVPSSQKVALMGDFTATWCPYCGEWGAPEFEKAIAGTGPAAIPLGIHNTDEITSE
                     VAQALTDYYGADGTVTGYPTLLVWNQGSFYDGELMAAAALTDAATGPAEAGAVASFTD
                     NGTAISISVSAETFADVTGDYFVVAYLMEDGIVRPQKINGLPDNPNFVHNHVIRASSN
                     GTPWGEQFIFAAGLNGSTFLKTYQVVKDPTWVIENMYMVAIVWKYDSSTESYTYVNAQ
                     ETHF"
     gene            15110..16933
                     /locus_tag="PLB46_00960"
     CDS             15110..16933
                     /locus_tag="PLB46_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRF75109.1"
                     /translation="MLKKTTILAFFTAITIVSFAQPAILLDNFATGLSAPVDIVNAGD
                     ERLFVVERYGYIKIVQPDGSVNPVNFLDIHTQVESGYQEQGLLGLAFHPNYAENGYFF
                     VHYNDLDGNTVIARFTVSTFDPDVADPTTELEIFTADQPFVNHNGGCLRFGQDGYLYI
                     GLGDGGSAGDPGDRAQNPENKLGKMHRLDIDSGSPYAIPASNPFAVAADTLPEIWAIG
                     YRNPWRFSFDKLTGNMWIGDVGQNLQEEVDVELAGDGGHNYGWRCYEGFDEFNDAGCD
                     DDISTYTMPIFQYPHNFTTGGFSITGGYVYRGSLYPGMYGYYLCADYVSGNWWWVDAD
                     GPLPWSYERMDDVATDISVIGEDVNGELYCADLSTGKIYKIQDACGDFVISVTGTDYT
                     CGTLDGAADLSITNGEAPYTIEWSNGAITEDISGLTTGNYTVTVIDNAGCERQKTVFI
                     NELPAFDVAVTVAGNTLTADNGISWQWYLDGVLIEGATSSTYDATTSGNYYVVATDIN
                     NCSATSESVALTVAINNIFINALKIYPNPTNDELFIQSPLGDHINYDIDLINQLGEIV
                     LQQKTNAELTKILLTTLTDGLYVVKLTANNTVYTQTIVLAR"
     gene            complement(17002..17847)
                     /locus_tag="PLB46_00965"
     CDS             complement(17002..17847)
                     /locus_tag="PLB46_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75110.1"
                     /translation="MRLKIILVTAILSVITFTSKAQSPGYMGKKFIVKVELLTTPTFY
                     KLNDINGYETYENVTSINGFGFSFTPSVGIEYVIGKGASAGIALRTSSLKMPMNYYEL
                     GSGYDEELEEYYTLGFVGETKLKTKMTSIYLKFYPHKKRGSIAPLGRFHQFELIVGGT
                     TYQNGEVIITNQTDPNAQLVGAGDYQNYILYDDINELSFEEKIPIQMLKYAYGYETII
                     KDKIPIEMSMQIAFSVAESFAELTGNVVSYESYGEFLFYENLNDRFSRSLNITFNIGL
                     GYLVF"
     gene            complement(17847..20048)
                     /locus_tag="PLB46_00970"
     CDS             complement(17847..20048)
                     /locus_tag="PLB46_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRF75111.1"
                     /translation="MRKLLLLLLTNCCILAASWSQQIDFENFTTLKSVGKIPEDFTTL
                     ASTKFKQDAAEIASENGREKADKEIFLLESNFLINEMLHSGRVIFGDPVTTYLNNIKA
                     EILKANPELDDDIRIYTLLSNDVNAFTADNGIVLVTTGLVAQAQNEAEIAFILCHEFS
                     HFYKKHAIDNYVENRKIERGIGVYSSLDNDAVDLEKFKYSKDLETEADVVGLSYYKKT
                     KYSLNAAENVFDVLLYSYLPFNEITFPREYFNEGPYVLPGKLFLDTLASITAAEDYDD
                     ETSTHPNIKKRKTAITDGIGNFSNDERIDYLQDVADFNYVQKICRFQGCEMYLYDIEY
                     ENAIYQAFLLQQEDSSNLYLKNVIAQSLYGMSMYKNASASQEWHRYYKKVEGESQQLF
                     YMGYKLNAKELNILALKYAWELYQTDSTNTALYKICVQLGDELVNENKIKTSEFLDAK
                     ATADYINKFQEQDSMSSLREVVTTDKPKTKYDKIKQEKAVPEENVEYWKFAFNKYIED
                     KAFLALLEEDVVINKKTKRKKSDGEYHLGLDEVVVVDPVYYSINEKLENPILYQDAEQ
                     AKIALKDKLKSNADELSLKLKYLDYHDLEANDTELFNDLSILNRWMNEKISHLGEEVF
                     MHTSTNDEFLALSNKYNIDDFAWMGIVSFTEEEKYLVAKIALCIYFPIAPFLIADLVT
                     PNRNTFFFTLVANAETGKLTMQYYNNTLQNDSHAVQMSNLYYILQQIKSKK"
     gene            complement(20069..20686)
                     /locus_tag="PLB46_00975"
     CDS             complement(20069..20686)
                     /locus_tag="PLB46_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75112.1"
                     /translation="MVEALLYKKLFLYLIPFNFLIGEYNFGMQYRFTDEWAVDINTGY
                     IGNIEGTYTSTVYENIFNSGTFYYSGPFIKTSILAVVPRGTNPLKTDYNQVEIAYRML
                     GYDELDFEDKTEKGKLFNISEDMQAISLSWKAGYNIIPREVFEINAFVGFGIQMRFKN
                     TTVNSYGYNYHSDMFEVNDITESMQTTPLFHAGIKVGLKTINNVK"
     gene            20839..>23124
                     /gene="lon"
                     /locus_tag="PLB46_00980"
     CDS             20839..>23124
                     /gene="lon"
                     /locus_tag="PLB46_00980"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRF75113.1"
                     /translation="MREFGFDDNFLSSMMEEDVEFLPLLSLEEDDDQIKIEYPETIAV
                     MPLRNTVLFPGVVLPITVGREKSIKAIQVAFKGNKLIGVVAQRDGNIEDPDTSQLYTT
                     GVIAKVIKQIKMPDGSTTVIIQGKKRFAVNKFTAQDPFLQASITLLEDNTKGDKKEFN
                     AIVSAIKDLASNIVKISPNIPQEANVVLKNINSPVFLMHFVSSNLQIDVADKQKLLET
                     DDLYLRAQQVLNFLNNELQMLELKNKIQNKVRTDLDKQQREYFLNQQLRTIQEELGGS
                     SQDEDIKRYKLRAAEKKWAKNVQEVFNKEIEKLQRLNPNAAEFGVITNYIEMLLELPW
                     GEYTADNFDLKHAKTVLDEDHFGLDKIKDRLIEYLAVLKLKGDLKSPILCLVGPPGVG
                     KTSLGQSIAKALGRKYVRMSLGGLHDESEIRGHRKTYIGAMPGRVVQSIKRVKSSNPV
                     FILDEIDKVGTDFRGDPSSALLEVLDPEQNSSFYDNYLELEYDLSKILFIATANNINT
                     IQPALRDRMEIIYLSGYSTEEKVEIGKRHLVPKQKKEHGLKQRDISLTDDVLTQIIEN
                     YTRESGVRDFDRQIASVMRRIAKYVATGEKYNKTIKQSEVEGILGTAKFDKESIFNTT
                     VPGVVVGLAWTPVGGDILFIETSLSKGKGTLQLTGNLGDVMKESASTALTFIKSHADE
                     LGLSPDIFEQTAIHVHVPEGAIPKDGPSAGITMLTSLASAFTGRKVKSYLAMTGEITL
                     RGKVLPVGGIKEKVLAAKRSGV"
BASE COUNT         7347 a         3982 c         4417 g         7378 t
ORIGIN      
        1 actatacatt tgttattccg gtttttgatg atattattga tgaatcagat gaaacattga
       61 ttttagattt aacttatatt tccggtgcat gcgctgtcgg tttgccaagt tcattcacat
      121 tgttaattac cgataacgac gatacgccac tcccagttta tactgaatat gatattgcag
      181 aagttactac tgaagatgct gatggtatag cattaagtat tgatgaaaaa gtagacttaa
      241 caggaatagt ttatggtatt aatacttggg acggcggatt acaatttaca ttaatcgaca
      301 ataccggagg aattagtgta tttagtttcg ataatacttt tggatatacg gttactgaag
      361 gtgacgaagt aaatatatta ggcacaattt ctcaattcaa cggattaaca gaagttgaac
      421 ctgatacact tatttttatt gatggtggaa atactttaaa aacaccaaca gaaataaccg
      481 tattaaatga atcaaccgaa tcagatttag tgagcataga aaatggtctt acctacgtgg
      541 atattacaca atggttgggc gatggtagtt cttttaatgt ggatttaaca gatggtacca
      601 atacctatac aatcagaata gacgatgcga atgaattatc aactatgcct gctccttcaa
      661 ttgttgaacc atttgtgcta atggtgacag gtcttggtaa tcaatttgat tcagatgcac
      721 catatacatc aggttatcag ttattccctc gttatgccga tgatattgaa ttgatttcag
      781 ttcttgaagt tgaagatgca actcaatctg gaattcaaat ttatccaaac ccggtaactg
      841 attattttat tgttgaaggg caacaaaaca taatcagtgt tgaattggtt aatcaatttg
      901 gtcaggtaat tttattagaa aataatcaat caaataccta ctcattaaac aatattgcag
      961 caggtattta cagcgtaaaa attacaacaa caaatggtgt tgtttccgac aacattgtga
     1021 agttgtaata aaatatttta ttaacatcca cattattctc atgatatgct cattaactgt
     1081 tgggcacagt agaataatgt ggattttttt ttaactgttt ttatgacttg aagttttaat
     1141 tccggcacaa taaaatccaa ctcctcctta tgtcaaaatt atcagcaaat gtaaaagccc
     1201 gcttccacca cgaaatgtat catggctggt ttaaaaaaag gcgttttttt gaaggttggt
     1261 atttcaaaat tgtagataaa tcggagcaac atgccctggc tatcattcct ggtatcgcga
     1321 tggatgaatc tggtgaaaaa cagagtttta ttcaggtgct ggatggtgtt aatgtaaccg
     1381 ctgcttacca taaatttgat gccatcgatt ttcggtctaa tgaaaacagg ttcaaagtac
     1441 gcattcgcga aaattctttt tctaccaaca gaattaaatt agacattccg ggcataaaag
     1501 gattgttaca ttttgatgaa cattttttct ggcctaaaaa aattatttca ccgggaataa
     1561 tgggttggta ttcgtgggtg ccatttatgg aatgttacca tcaggtagtt agcatgaatt
     1621 gtgcagtaac cgggaaatta atcatcaata aaaatgaagt tgattttgta aacggaagag
     1681 catatattga aaaagactgg ggtagaagtt ttccttcgtc gtggatttgg atgcagagta
     1741 atcattttac taaacaacct gtttctttta aattatcagt agcgaaaatt ccatggctga
     1801 gtggtgcgtt tgtcggattt atttgtgctt tcttaatcga taataaatta attcgttttg
     1861 caacatatac aggcgctaaa ttaaaatcga taagcgcaga aggagaaaca atcgatatcg
     1921 ttttagaaga cgctaaatac gtgctaaccg tacaagccca aaaagcagca ggtgccgaat
     1981 tggccagccc tgtattaggt ttaatggaag gccgcgtaaa agaaagtatg cgggcactta
     2041 ttcacattac acttttttcg aaacaggaaa ataaggttat ttttaacgac acaggtcgta
     2101 atgcaggatt agaaatagcc ggcaatacga ctgagttgct aaattttaag taagaacatg
     2161 cgcaatctgc ttttcttgat attttttatc cccatacttg tcctggcaca ggatacttta
     2221 ccctacagtg aatttcgatt tacaattgtt gaagtaaacg ggaacgactt aaccgatgat
     2281 aaaattgaag cgcgtattgc agaagtattt tataccgata ccagcaaaca cgatttggat
     2341 tatgtgttta cccaattagc ttattccaaa aaagatagtt gctggatttt aaaacaaaac
     2401 gaacctttgg gatataatta taaaattgaa attttcagaa aatctgatac cgttaatccg
     2461 cggttactgg aattacgaaa aatgaccctt atttataatg gttacgaccc tgaaaataaa
     2521 tcaggatgtc aatattgcat ttgcaacgaa atacccatgg aaaaaggtgt atttgtgatt
     2581 gatgttccgc gtaaaatgga gtcatgggca tatatccgaa aagtagctat caatgtaaaa
     2641 aatgttccaa ccgaatttcg tgatattacg gctattcaaa attggttttt ccgaaaaaat
     2701 aaataatagc cgtcgcccat tgtgttatgg aaaatctaca accttattac gacctagttg
     2761 aatcagtaat cgaatcgttt ggtatcaatg ctaccgaatg tcgtggcgaa aaatcaggca
     2821 tgtggaaaat gtataaaggt tctgcagaag tgtggatcga cgtgtggtat atcgaaaaag
     2881 aacaaaggag ctactttcag gtgatggcac cggtaatgcc tgttcctgca caaaaccggg
     2941 aaggttttta cctcgaatta ctggaactta gtgatggcat gttcgatgtt gcttttagca
     3001 ttcacaaaga ttgggcttat attaaaaata tccgtgaagc cgccggtatc gacttcaccg
     3061 aaattcgttc aacaatcaac cgtgtaggta gttattgcga tgaacacgac gaccgcttga
     3121 aagctaaata tggccctccc caggcaccgg gcgctcctcc aaccaaataa tggatggttt
     3181 ttaccagtcg aatacctaca aaaaggtata acattttgag tcctttattg gttacttttg
     3241 taccttattt agatttatta taaacaaatg gctcgaattt taggtaagca actgctgcaa
     3301 atgcctgtat ttattggtat agctgccatt ttaacgcttt tttcctgcaa acctaccgaa
     3361 cctgacacga catccctgaa aaaagaaatt attaaaactt attctcaggt tgtttttgcc
     3421 acatatgaag atgcccttac taccgcaaaa ttgcttcaaa gtgctgttga tacgttttta
     3481 atcaatccaa ccgaattaca tcttaaagct gctcaaaaag cctggattac cgccagagca
     3541 ttttatggtc aaacagaggt tttcagattt tatgatggtc cgatagataa tggagaaaca
     3601 ggagtagaag gtttgctcaa cgcatggcca ttggatgaag tttatgtgga ttatgtggta
     3661 ggtgaaccaa atgccggcat cataaataat gttcaggaat atcctgcaat tacccgcgat
     3721 ttattagtta gtttaaatga aaaaggtggt gaggaaaata ttagttgtgg ttaccatgcc
     3781 attgaattta tgttgtgggg gcaagatgta agtgataaaa aaccggggca gcgaccatat
     3841 accgatttta ttcccggtac agttagcaac gaccgaagaa gaacattttt gcaactcaat
     3901 accggattgc tgattgaaca tctcaccaaa ttatgtgatg catggcaacc tggaaaaact
     3961 gataattatg cagccaaatt tttaagcgta aaccctgatg aagcattgca aaaaatatta
     4021 accggaatgg gcgtattgag caaagtagaa atggctggtg aacgtatgtt taccgcttac
     4081 gataatgcca gtcaggaaga tgaacaaagt tgtttcagcg ataatactaa agccgattta
     4141 caaaataata ttggcggaat tataaatgta ttttatggta attatgtgcg atacgataaa
     4201 acaaatgtag atggtgctgg tatcgacgat ttattgaaaa ataatcactc agcaatgcta
     4261 gacagtacgg tattgatgtt aaaaaaatgt aataacgata ttcaaaatat ttattacccg
     4321 tttgatcagg caatcgttgt ggaaaataaa cgtgaacagg ttatgaaagc gattttaacc
     4381 acccagcagt tgggtgaaca attttcaatt atagccgcaa ctttaggtat cactataaat
     4441 gtaaatgcag cagattaatg aaatttaatt ccaataaaat aaccattttt actattacag
     4501 cagtaatggt attatttatt ggtatatcat tttttgcctg cgaaaatttt gttcctaata
     4561 atgcagcaac cgctttagtt aatcaaccta atgaaacatt atccggtggt gctgcaacag
     4621 tttttgatca atcagtaaat gcatttgcat taccaatacc taccttatca tcacaagatg
     4681 aattattatt ttttgtcggt aattcttttt ttaatcagaa ctgggtacaa gcgccttcca
     4741 gcacaacagc acgtgatgga ttaggtcctt attttaatgc aaaatcatgt tccggatgtc
     4801 actttaaaga tggcagaggg cagccaccgg caagtattgg acaattatca gaaggatttt
     4861 tattgcgatt gagtgtgccg ggaactgata aaaatggcgg accagtcggc gataaaaatt
     4921 atggcctcca atttcaggat catgctattg atggtttatt ggcagaaggt atttttcaat
     4981 tgcaatggga agaaatttcg ggcaaatatc ctgatggaac aacttaccat ttgcgcaaac
     5041 ctaaaattat tttcgatgaa cttaattatg gtgaaatgag tagcgatatt ttgatttcac
     5101 cgagagttgc tccacaaatg attggactag gattattgga agccattgat gaaaaaacaa
     5161 ttttggcttt cgttgacgaa caggataaaa ataatgacgg cattagtggt cgtgccaatt
     5221 atgtttggaa tcatattaca cagcaacaac aaatcggtcg cttcggatgg aaagcaaatg
     5281 agccaactat tttgcaacaa atatcaggtg cttttaatgg tgatatggga attcgcacac
     5341 aatattttaa agaccaacat atacttcctg cacaaaaaaa agcagcaaca ttaccgaatg
     5401 gtggcgaaac ggaaattgat aacgacgatt tagaaaaatt acatttgtat tcagccactt
     5461 tagctgtgcc tgcacgaaga gatgtgaacg acaaaaatgt aatagcagga aaaaatattt
     5521 ttatggcatt aaaatgtaat gcctgccata ttgataaaat ggtaaccgga acacatccaa
     5581 aatttactgt tttgtctaat cagaccattc agccatttac cgatttgtta ttacacgata
     5641 tgggtccggg tttagccgac aatcgccccg attttttagc cagtggaaac gaatggcgca
     5701 caccaccact ttggggaata ggattattta aaaccgtaaa taaacatacc ttctatttac
     5761 atgatggccg cgcaagaaat ttagaagaag ccattttatg gcacgacggc gaagctgcca
     5821 atgcaaaaaa cggttttatg caattaaaag aatcagagcg caaacaatta cttcaatttt
     5881 tgctgtcact ttaataaaaa caatatttat agctaatttg cgttctgttt gttgaccaca
     5941 gctaatggaa ttcttattta cattactttt tcaaatacag ctttgtttgc cggcgcccac
     6001 aaatgcggta agcagcccgg ttgctataaa taaattgtcg acacctgaat ttctggtagg
     6061 taaacataac tattacaccg atacaaattt tgtagaaatt ccggctcata tggcctatag
     6121 gactgaaatg tttttattag atgaggttta tgctgcatat tttgaaatgt atactgccgc
     6181 aaaaaaggaa ggtgttaatt tgaaaattat ctctgcttcc cgaacatttg aagaacaaaa
     6241 gtggatttgg gaagataaat ggaagaaaaa taaagcaact taccctgccg ataccagcct
     6301 ggcaaaattc atcatgcaat acagcgcaat gcccggtaca agccgtcacc attggggtac
     6361 agaagttgac ctcaacagca cttcagatag ttatttcgcc actgcagcag gtgcaaaagt
     6421 gtataaatgg cttaccgatc atgctgccga atatggtttt tgtcaaacat acgattcgaa
     6481 aggcataagt cggcacgaag gttacaatga agaaaagtgg cattggagct attttcccat
     6541 cgcagatcca ttgctgaaaa aatatgagga attggtggaa tactcccata ttcagggctt
     6601 ttcaggcgat aatgcggcgc caaaactcga tgtaatcaac aactatgttt tgtccataag
     6661 tcacgaatgc gaaaaatagt tgtcgaaaaa attgcgtatc agatattttt gtatctttga
     6721 taaagagttt cgttagtttg ttcattgctg cagttttttt actcgccgtt ttttggactt
     6781 attcatttgc aactcctatc ttttattatt aacaaattag aaaaaaatga acatttacgt
     6841 ttcaggattg agtttcaaac tcaatgacgc cgatttaagg cagttgtttg aagagtacgg
     6901 tgaggtatct tcagctaaaa ttattatgga caaatttaca ggccgtagcc gtggatttgg
     6961 tttcgttgaa atgaacgatg aagatggcca aaaagctatc gacgcgttaa acgaagctga
     7021 agtagacgga aaagttatcg gcgtatcagt tgctagacct cgtgaagaac gtcctcgcaa
     7081 taacttcaac cgcggtggcg gtggaggcgg tggcttcaac aaaggtggcg gcggtggtgg
     7141 ccgcaataac gatcgcgatg gcggtggtta tggcaagaaa aacttcaacc gttggtaatt
     7201 attaagtcaa aataatacaa aaccctgttc gttttcggac agggtttttt gtttgttaaa
     7261 aagacgctta acttagcctt aataattatt taatttacgt actcgtatta acatgcgcta
     7321 cacacttacc tcctttttag taataattac tgccatatcg tttgctcaaa gtttttatca
     7381 gttagaaaat accgatatcc ccgacgatgg aacatccatt atgtttgaaa tgaacgtgtc
     7441 cggtttacct tccactatag atggcgattt tgggttagaa tccgtttgtt ttaatattaa
     7501 ccatacctgg gacagcgata ttgatgtttt attaatagca cctgatggca gctcttttgt
     7561 attggtgagc ggtgtgggtt gggctgcagt caattttgaa aatacgtgtt ttaatagctt
     7621 cgcagataat tttatttcag atggtgttgg cccatttacc ggcgaatgga aaccaatagg
     7681 cgacatgggt gctgtaaata acgggcaaaa tccgaatggg atttggaaac tgtatgtata
     7741 tgatacatgg gcttttgccg acgctggatt tatgatcgac tggtccatca cttttggtga
     7801 taatccggca attccttttc ctttttatag ttctaattta cctatcgtaa aaataaatac
     7861 cttgggtgca attatgaatg agcccaaaac cgaagctagt ttatatatta tagatggtgg
     7921 cattggcgca atcaatattc cttccgatac aaattatgct tttgaaggaa aaatattagt
     7981 tgaattgcaa ggcttctccg ggccttatta tcccaaaaaa aattatgact tcgatattgt
     8041 tgatgatgcc ggcttagaat acgataccgt tttattaggc ttaccttccg aaaacgattt
     8101 tattttaaaa gcagaatatt tggattattc gttaatgaaa aatgccatta cttacgaaat
     8161 gtatcgcaaa atgggacgat acgctccacg cactaaatat tgtgaagtaa tggttaacgg
     8221 cgaatataaa ggtgtttatt cccttacgga aaaaattaaa cgcgatgaaa acagagtgga
     8281 cattgcccaa ttaaatcctg aagatatttc cggcgaagaa ttaaccggtg gatatatttt
     8341 tgaaatcaac gaaaattata cacccaacga ttgggaaagt gattacctgc caattaatta
     8401 tgcgtcatgc ggtttacctg ttgcatataa aatggtgtat cctcgcgtgg aagaaattca
     8461 accggaacaa ctcgattata ttgtggccta tgtcgatagc tttgaagatg ctttgtatgg
     8521 cgatgaattt ttagatgaag caaatggata tcgcaatttt atttcggtaa aatcttttgt
     8581 tgatttttta atcacgaatg aatttagtgc gaactatgat agttatggca gaagcacctt
     8641 tttacataaa gacaaaaatg gagaaataaa tatcggaccg gcatgggatt atgaccgtgc
     8701 ttttgcacct tgggatgtaa gcggttgggt ttgggaaaat acacatcccg catggccttt
     8761 tccattttgg tggagtcgtt tgcaagatga tgaagaatgg agaaatgaag tgtattgccg
     8821 atataacgat gtgcgtcaac atattttaac cgacgaaaat gttactgcat tcatcgattc
     8881 cgttgatgcc tatattggtg atgctgctgc acgtaatttt ttaaaatggc aggaactagg
     8941 tgtaggtgac cctgaatatt ttgtaacgga attgcgcgac tttattgatg cgcgattaaa
     9001 ttggatggat attactttgg ccgcagattt tgtgcctgca ccagatgcct cttttacttc
     9061 aactagttta ggagcgctta catacgcttt cactccggtt actactaatg ctgattatct
     9121 ctgggatttt ggtgatggca tacaatcaac cgaagaaaac ccaatacata catttagcgg
     9181 tgccggcgat tatcttgttt cacttacagt aaataaatat tatggctgca ttggtaactc
     9241 agatgaaatg tttcaggcca atgtttcaat tgcagatatg ggtttgaatg gaattaccat
     9301 tttcccaaac ccggctactg attttattca agtcgagtca aacaataatg tagtaattaa
     9361 tgaaacagca ttgtttacgg tagaaggaaa atatattgaa acaaaaatca gtaccaccgc
     9421 aaatgggatt gtaatcgacc ttaaaaatgt tgccgcaggg acttatttat taaaaatcaa
     9481 atccggcgaa acagtaatca attacccgtt tattaaatat taaaataagg tattcaatta
     9541 ttaacgcagt cagcaaacat ttaaataggt ttataaatgg tgccaatata atccacaaaa
     9601 tcaggagtta aaccttgttg ccggatgaat aattgcattt gcgctctgtg atgtatgcta
     9661 tgataattta attgcaatgc caggtctttt agcggcgatg tatagggttt attgccaaca
     9721 ccaatccaaa ctacattttc caataactca gcttccgatt tttctgcaag caaatcgagc
     9781 catggttgta aacttttgtc ccaatatgac gctaattcgt ccatcgggta aacaggattc
     9841 caccagtcga gtgttgatgt tgcaggataa attcgaatac gatccatcca cttaaactga
     9901 caattaatca ggtgactaaa atgtttgata cactcactcg gatctggcat ttgctgcatc
     9961 ttttcgagta ccattttatt ggctttgtcg ttaaataaaa aagtgtcggt gagatattgg
    10021 ttgagttgca tagctgaaaa attcgaacga atatattaaa tttcagtaat tcattaagat
    10081 tttttgatta aaaaatttac ccgtagaaat attccattca agtaaatgct gaaagtcaat
    10141 ccaaattcat ttcatcataa acattcaaga ttactttaac ataaaggtag ctttaaaaca
    10201 attttataaa acatgtagtc taaccggagt gtatcgtttc gatgttataa gcagtgacaa
    10261 tgttcaatac ggctatttaa tttgccaatc agttttaatt atatattctg aatagcaatc
    10321 ctgcgttttt cttttaattg tttaaaatgt gttggcgtaa gccctgtagc ttttttaaac
    10381 tgtgcagata ggtgtgccac actgctatag tgcagcatat aggcaatttc tgataaggtg
    10441 tgttcaccaa aaataataag ttctttgata cgctcaattt taagtccaat catatactgc
    10501 tcaatagtgg ttgcttcaac ttcactgaaa aagttagcga gataggtata actgtgattt
    10561 aactctttac ttaatagtgc tgaaaatttc atggtgggtt tttcttcggt attatataca
    10621 taatcgataa ttacctgcct gattttttcg actaaaacac cctgtttttt ttccaataat
    10681 tctaacccaa cttttttaat ttttttgttc aatttcagct tgtcttcatc cgagatttct
    10741 tctttggttt cgatttcacc taattctact tttacgggct tgatatctaa ttcttcaagt
    10801 gcttctttaa caacaacctt gcaactttca cacgccatat tttttacgta aatcttcatt
    10861 taacaggatt ttgagatgta atttacaacc tataagtgat attgtcaaaa aaagctaatt
    10921 gttcaattat tacttatagt tgtaattatt acaacatgta tttattgaca aataattaaa
    10981 ataacgcata ttgacaatta ttccggggac tagtaattta cggatatatc caacatattc
    11041 gtttgatgaa tgtattatta tcggtaaatg agtttttatt tagccgatgt atttatctga
    11101 ttttgaactt gttggaattt gttacaaata gttgttttgg cgtttgggaa ttaaaatacc
    11161 gatctgtaat atattaaaat gataaaaccc cgtccgaaga cagggtttca tgttataatt
    11221 ttgataaaaa ttaatttgct tctacgccac aatatttatt ataggcattt gctaaatcat
    11281 tggcaatcat catcgcattg cgaccttcaa tttggtggcg ttcaataaaa tataccaatt
    11341 tgccactttt gaaaagtgca atactaggtg aacttggagg gtaggggagc gttaatttac
    11401 gtgcttctgc aaccgcatca agatcaaacc cggcaaacac tgtcgctaac ctatcaggtt
    11461 tattttcatt gcttagcgaa aggcgaacac ccggacgggc attacctgct gcacatccgc
    11521 aaacagagtt gataacgagt aatagagttc cttctgtgcc tttaattgtt tcttgaacca
    11581 tttcaggcgt agttaagtcc tcgaagccca ccttggtaag ctcttctttc attggttgta
    11641 ccagttcttt tggatacata gtttctgcta ttttaagtta caaagttacg tgattgatag
    11701 attttaacct attctataaa aatgtatgtt attccatgaa ccaacatcta ctataaatgg
    11761 ttcaatcatt gccaaaggga aagcaatttt ttacctttgc atctaagttc aaatcagtaa
    11821 tcattaaaaa caacataatg aacgtagcaa cagacaaaaa actaccatac aaagttaagg
    11881 acataacctt agctgcatgg ggtcgcaaag aaattaaact ggcagaagcc gaaatgccgg
    11941 ggctaatgag cctccgcgag gaatatggca actcaaaacc gcttaaaggc gctcgtattg
    12001 cgggttgtct gcacatgact atccaaacag cggtgcttat agaaacactt aaagaattag
    12061 gtgctgatgt acaatggagt tcatgtaata tcttctctac tcaggaccat gctgctgctg
    12121 ctattgccgc tgccggaatt cctgtttatg cctggaaagg tgaaacttta gaagaatatg
    12181 actggtgtat agaacaaact ttgttcttcg gtgaaaataa tgaaccatta aacatgattc
    12241 ttgacgatgg tggtgatctt accaacctgg ttttagataa atatcctgaa atggtaaaac
    12301 accttaaagg tatttctgaa gaaacaacca ccggtgttca cagattatac gatcgcgttg
    12361 tgaaaggcac tttacctgtt cctgccatta acgtaaacga ctctgtaacc aaatcaaaat
    12421 ttgataataa atatggttgt aaagaatcat tagtggatgc tattcgtcgc gcaactgata
    12481 ttatgatggc cggaaaagtg gctgttgttg ccggttatgg cgatgttggt aaaggctctg
    12541 cacaatcatt acgtggttca ggtgcgcgtg ttttaatcac tgaaattgac ccaatttgtg
    12601 cattacaggc tgcaatggat ggttatgaag tgaaaaaaat ggacgatgcc gttaaggaag
    12661 ctgatattat tgttacagct accggtaact gtgatattat tacaggacgc cattttgaaa
    12721 atatgaaaca caatgctatt gtttgtaaca taggccactt cgatgatgaa attgatgttg
    12781 catggttaaa caataatttt ggtcatacaa aagacgaaat caaacctcag gttgataaat
    12841 ataccgttaa tggtaaagac cttatcctat tagcagaagg tcgtctggtg aacttaggtt
    12901 gcgctatggg ccacccaagt tttgtaatga gtaattcatt tactaaccaa acattagcac
    12961 aacttgaatt atggttaaat ggtggtaatt acaaaaacaa agtatatacc ttacctaaac
    13021 atttagatga aaaagttgcg cgtttacact taggaaaatt gggtgttgaa ctcgagattt
    13081 taagcgataa acaagctagt tatattggcg ttgaaaaatc cggaccatac aaaccggaat
    13141 attaccgcta ttaatttttt taatcttgct ttcaaaaggg gtgacagaaa tgttgctcct
    13201 tttttttatt cctgaaaccg ggatttatag gattattatt gtcgacaatt tggtaaatga
    13261 gcaatatttg tcttgcaggt taaaaaggtt ttgtttcttt gtaattcaat attaaaccgc
    13321 aatgacaaaa ttatctgtca atatcaataa aattgccaca cttcgcaacg caagaggtgg
    13381 taacgtgcca aatgtagttc agtttgcaaa agattgtgaa cgttttggtg cagatggtat
    13441 taccgttcat ccccgcccgg atgaaagaca tattacacta aaagatgtat atgatttagc
    13501 tccggtagtt aaaactgaat ttaatatcga aggttatccg gatgccagat ttatggaaat
    13561 aattcgtacg attaaacctg cacaggcaac actggtgcct gatccaccta atgttattac
    13621 ttcgaataca ggatgggatg ttatggcgaa taaacaattt ttaatagatg ttgtgcacga
    13681 aattcaaagc tatggtgttc gtgtatcaat atttttaaat ccggaaatac atcaggtaca
    13741 cgaagcaaaa ttaacaggca ctaatcgcat tgaattttat accggccctt atgcggaaca
    13801 ttattttaaa gataaacact ccgctgttgc actttacgca gatgcagcta aggcggctta
    13861 tgaaattgga ctgggaataa atgcgggcca tgatttgagc ctgcaaaatt tacaattctt
    13921 caaacaatca ttattacata ttgatgaagt atctatcggt catgctatag ttagcgatgc
    13981 gttatattat ggaatagaaa atgtaataca aatgtataag cggttactac aataacataa
    14041 agtaaataat aaaacacaaa atacaaatca taaaaaaaag cctcccgaat tggaaggctt
    14101 ttttttatga taagtttatt gattaaaagt gagtttcttg tgcatttaca taagtgtaag
    14161 attctgtaga tgaatcgtat ttccaaacaa tagcaaccat atacatgttt tcaattaccc
    14221 atgttggatc cttaacaact tgatatgttt ttaaaaatgt tgatccattt aatcctgcag
    14281 caaaaatgaa ttgttcgccc caaggtgtgc cattggaaga agcacgaatt acgtgattat
    14341 gaacaaagtt tggattgtca ggtaagccat taattttctg aggtctaaca ataccatctt
    14401 ccattaaata agcaacaaca aaataatcac ctgttacatc agcaaatgtt tctgcgctaa
    14461 cagaaataga tattgctgtg ccattatcag tgaaactagc aactgctcca gcttctgccg
    14521 gacctgttgc agcgtctgtt aaagctgcag cagccattaa ttcgccgtca taaaaagagc
    14581 cttggttcca aactaataat gtaggatatc cagttactgt accatctgct ccgtaataat
    14641 cggttaatgc ctgagctact tcgcttgtaa tttcatctgt attgtgtata cctagaggta
    14701 ttgcagccgg gcctgttcca gcaatcgctt tttcaaattc aggtgcaccc cattcaccac
    14761 aatatggaca ccatgttgca gtaaaatcgc ccattaaagc aactttttgg ctgcttggaa
    14821 caaaacaagt accatcatca tcagttgcat ttggattata actgattgaa aatgggtcgg
    14881 tacaaccgta aatacggtct tttgcgcatg aagagaatgc caatgcagat gttactgcta
    14941 ctgccacaat aagaagtttt ttcatataca gaattaattg gtttttggaa atccgagact
    15001 aaagtaacaa atatttgtgt atatacaaac aattttatgc acaaatctga cacaaacctg
    15061 acatgctctg caaatcagct tatgctatct ttaccaaaaa tatggttaaa tgttgaaaaa
    15121 aacaacaatt cttgcatttt tcactgctat tacaatagtg agttttgccc aaccggctat
    15181 cctactggat aattttgcta ctggcctttc tgcaccggtg gatattgtaa atgcaggtga
    15241 cgagcgcctt tttgtggtgg aacgttacgg ctatatcaaa atagtgcagc ctgacggaag
    15301 tgttaatccg gttaatttcc ttgatatcca tactcaggtt gaatccggat accaagagca
    15361 aggtttattg ggattggctt tccatccgaa ttatgctgaa aatggatact tttttgtgca
    15421 ttataacgac cttgacggga atacggtaat agccaggttt acggtgagca ccttcgatcc
    15481 cgatgtagct gaccctacca ctgaattgga aatttttact gccgaccagc cttttgttaa
    15541 tcacaatgga ggctgtttac gatttggtca ggatggttat ttgtatattg gtcttggcga
    15601 tgggggctct gctggtgacc cgggtgacag ggcacaaaat cccgaaaata aattaggaaa
    15661 aatgcatcga ttagatattg atagtggtag cccgtatgcc atccctgcct caaatccatt
    15721 tgctgttgca gctgatactt tacctgaaat atgggctatt ggttaccgca acccttggcg
    15781 ttttagtttt gataaactca caggcaatat gtggattggc gatgtaggtc aaaatttaca
    15841 ggaagaagta gatgttgaat tggccggaga tggtggtcat aattatggat ggagatgtta
    15901 tgaaggtttc gatgaattta atgatgccgg atgtgacgac gatatttcaa catacacgat
    15961 gcctattttc cagtatccgc ataattttac aaccggaggt tttagtatta ccggtggata
    16021 tgtatatcgc ggaagtttat atcccggcat gtatggttat tatttatgcg ccgattatgt
    16081 ttccggtaat tggtggtggg tagatgcaga tggtccactt ccatggagct acgaacgtat
    16141 ggatgatgtt gcaactgata tttcggtaat tggtgaagat gtaaatgggg aattatattg
    16201 tgcagattta agcacaggaa aaatatataa aattcaggat gcatgtggtg acttcgttat
    16261 ttcagtaaca ggtactgatt atacgtgtgg aactttagat ggagctgctg atttatctat
    16321 caccaatgga gaagcgccat atacaattga atggtcaaac ggagcaatta cagaagatat
    16381 ttccggatta actaccggta attatacagt taccgttatt gataatgctg gttgcgaaag
    16441 acaaaaaact gtttttatca atgaattacc ggcatttgat gtagctgtta ctgttgcagg
    16501 taatacgctg actgccgaca acggcataag ctggcaatgg tatttggatg gtgttttaat
    16561 tgaaggtgca acatcatcaa cttacgatgc aacaacatcg ggaaattatt atgttgttgc
    16621 cacagatatc aataattgtt cagcaactag tgaatctgtt gctttaacag ttgcaatcaa
    16681 taatattttt ataaatgctt taaaaatata tcctaatcct acaaatgatg aattatttat
    16741 tcagtcccca cttggagacc acatcaacta tgatattgat ttgataaatc agttaggtga
    16801 aattgttttg caacaaaaaa caaatgctga attaactaaa atactactta ctacattaac
    16861 agatggtttg tatgtagtta agttaaccgc aaacaatact gtttacacgc aaacaatagt
    16921 gcttgccaga taaatgaaac tataataaaa agcgccttgt tctttttaag agcaaggcgc
    16981 ttttttattt agaagtgata attaaaacac taaatatcct aacccaatat taaatgtgat
    17041 gtttaatgaa cgactgaaac gatcattcaa attctcataa aacaggaatt caccataaga
    17101 ttcataagaa accacattgc cggttaattc agcgaaggat tctgcaacac tgaaggcgat
    17161 ttgcatactc atttctatcg gaattttatc ttttattatg gtttcatatc cgtatgcata
    17221 ttttaacatt tgaataggaa ttttttcttc aaatgataat tcattaatgt catcgtataa
    17281 aatataattt tgatagtcac ctgcaccaac taattgggca ttagggtctg tttgattagt
    17341 aattatcact tcaccgtttt ggtaggtagt gccacctact atgagttcaa attgatgaaa
    17401 tctgccaagt ggagctattg agcctcgttt tttgtgtggg taaaatttta aataaatgct
    17461 ggtcattttt gtttttaatt tggtttcacc cacaaaacca agtgtataat actcctcaag
    17521 ttcttcatca taaccactgc ccagttcata ataattcatt ggcattttaa ggctacttgt
    17581 tctcaaagca ataccagccg aagctccttt gccaataaca tattcaatac ctactgaagg
    17641 ggtaaagcta aatccaaaac catttataga agttacattt tcatatgttt cataaccatt
    17701 aatgtcattc aacttataaa atgttggtgt agtaagcaat tctactttaa caataaattt
    17761 tttgcccata tagccgggcg attgagcttt tgaggtaaat gtgattacac tcaaaattgc
    17821 ggttacaaga attattttaa ggcgcattat tttttagatt taatttgttg aagaatataa
    17881 tacaaattgc tcatttgaac agcgtggctg tcgttttgca gggtattgtt ataatattgc
    17941 attgttaact tgcctgtttc tgcattggca accagtgtaa agaagaatgt atttctgttt
    18001 ggtgttacta aatcggcaat cagaaaaggt gctattggaa agtaaataca taatgctatt
    18061 tttgcaacta gatatttttc ttcttcagta aatgaaacaa tgcccatcca ggcaaaatca
    18121 tcaatattat atttattcga caaagctaaa aattcatcgt tggtagacgt atgcataaat
    18181 acttcttctc caaggtggct aattttttca ttcatccagc gattgagtat agaaaggtca
    18241 ttaaataact cggtatcatt ggcctccaaa tcatggtagt caagatattt cagttttaat
    18301 gatagctcat ccgcattact ttttaattta tctttcaatg ctattttagc ttgttcagca
    18361 tcctgatata gaattgggtt ttctaatttt tcattaatac tataataaac cggatcaaca
    18421 actacaactt cgtctagccc caaatggtat tccccatcag attttttacg ttttgttttt
    18481 ttatttatca ccacatcttc ttcgagtagc gctaaaaacg ctttgtcttc aatatatttg
    18541 ttaaatgcaa atttccaata ttcaacattt tcttcaggca ctgctttctc ctgttttatt
    18601 ttgtcatatt ttgttttcgg tttgtcggtt gttacaacct ccctaaggct agacatactg
    18661 tcttgttcct gaaatttatt tatataatct gcggttgctt tggcatccaa aaattcagat
    18721 gttttgattt tattttcatt gactagttca tcgccaagct gcacacaaat tttatataag
    18781 gcagtatttg tactatcagt ttgatataat tcccaagcat atttcaatgc caatatgttg
    18841 agttctttag cgtttaattt ataacccatg taaaacaact gctgcgattc gccttctact
    18901 tttttataat aacgatgcca ttcctgtgaa gcacttgcat ttttatacat actcatgccg
    18961 tacaaagatt gtgcaataac attttttaaa taaaggttgc tgctatcttc ctgttgcaat
    19021 aaaaaggctt gataaatagc gttttcatac tcgatatcat ataaatacat ttcacaaccc
    19081 tgaaacctgc aaattttctg tacataatta aaatcggcta catcttgcag gtaatcaatt
    19141 ctctcatcat tactaaaatt gcctatacca tcagttattg cggtttttcg ttttttgata
    19201 tttggatgcg tgctggtttc atcatcataa tcttccgctg ctgttatgga tgccaatgtg
    19261 tctaaaaata atttgcctgg taaaacatat ggtccttcgt taaaatactc acgcggaaag
    19321 gtgatttcat taaaaggtaa ataagaatac aataacacat caaatacatt ttcggcagca
    19381 tttaagctat acttggtttt tttataataa ctcaaaccaa ccacatcagc ttcagtttct
    19441 aaatctttag agtatttgaa tttttccaaa tccactgcgt cattatctaa actgctgtag
    19501 acaccaatac cacgttcaat tttacgattt tcaacgtaat tgtcgatagc gtgtttttta
    19561 taaaagtgac tgaattcatg gcaaagtata aaggcaattt ccgcttcatt ttgtgcttgt
    19621 gcaaccaaac ctgttgtaac cagaacaatt ccattatcag cagtaaatgc attcacgtca
    19681 ttagacaaga gcgtataaat acgtatgtcg tcatcaagct caggatttgc tttaagtatt
    19741 tctgctttaa tgttatttag ataggtagta accgggtctc caaaaattac acgtccactg
    19801 tgtaacattt cgttaatgag aaaattagat tccaataaaa agatttcctt atctgctttt
    19861 tctcgaccgt tttcacttgc tatttcagca gcgtcttgtt taaattttgt tgacgccagg
    19921 gtagtaaagt cttcaggtat tttaccaacg gacttgagtg ttgtgaaatt ttcgaaatca
    19981 atttgttgtg accagcttgc tgctaatatg caacagttgg taagtaaaag caaaagtagt
    20041 ttccgcatat gttttctgaa gttgtatttt atttaacgtt attaattgtt tttaatccta
    20101 ctttaatacc tgcatggaat aatggcgttg tttgcatcga ttctgtaata tcattcactt
    20161 caaacatatc tgaatgataa ttataaccat aactgtttac ggtggtattc ttaaatcgca
    20221 tttgaatacc aaatcccaca aatgcattta tttcaaatac ttctctcggt ataatattat
    20281 aaccggcctt ccacgataaa ctgattgcct gcatatcttc actgatgtta aataattttc
    20341 ccttttcggt tttatcctca aagtccaatt cgtcgtaacc cagcattcta tatgcaattt
    20401 ctacttgatt gtaatcagtt ttgagtgggt tggtgcctct gggaactaca gctaaaattg
    20461 aagttttaat aaatggaccg ctataataaa atgtgccact attaaaaata ttctcataaa
    20521 cagtactagt gtaggttcct tcaatgttgc caatataacc cgtattgata tcaactgccc
    20581 attcgtcagt aaaacgatac tgcatcccaa aattgtattc tccaatcaga aaattaaagg
    20641 ggatgaggta gaggaatagt tttttataca atagggcttc aaccattgtt atgcaaatct
    20701 atcaatttgt cagtaaaacg acgacttttg gcagattttc ttgcatttgt cagcaaattc
    20761 tgtccgaggc acgacaattg agtgctaatt tggcaaccgg catttttggt gttagtttta
    20821 acttttcaaa gaaaaaaaat gagagaattc ggatttgatg ataacttttt gtcctctatg
    20881 atggaagaag atgtggagtt tttgccgtta ttatcactgg aagaggatga tgaccaaata
    20941 aaaattgaat accccgaaac tattgcagtt atgcctttgc gcaatactgt attgtttccc
    21001 ggcgttgtgc tgccaataac agttggtcgt gaaaaatcca taaaagctat tcaagtggca
    21061 tttaagggga ataaactgat tggtgttgtt gcgcaacgcg atggcaatat agaagaccct
    21121 gataccagtc agctttatac caccggcgtt attgccaagg tgattaaaca gataaaaatg
    21181 cctgatggca gtaccacagt aattattcaa gggaaaaaac gttttgctgt taataaattt
    21241 actgcacagg acccatttct tcaggcaagt attaccttgt tggaggataa taccaaaggc
    21301 gataaaaaag aatttaatgc tattgtatca gccattaagg acctcgcttc taacattgta
    21361 aaaatttcac ctaatattcc gcaggaagct aatgtagttt tgaaaaatat taatagtcct
    21421 gtttttttaa tgcattttgt ttcatcgaat ttacaaattg atgttgccga taaacaaaaa
    21481 ttattggaaa ccgacgattt gtatttgcgt gcgcaacaag tattgaattt tttaaataac
    21541 gagctgcaaa tgctggagct caaaaataaa attcaaaata aggttcgcac cgatttagat
    21601 aaacaacaac gtgaatattt cctaaatcaa caattgcgca ctattcagga agaattgggt
    21661 ggaagtagtc aggatgaaga tattaaacga tataaacttc gcgcagcaga aaaaaaatgg
    21721 gcgaaaaatg tgcaggaagt atttaataaa gaaatcgaaa aactccaacg ccttaatcca
    21781 aatgctgccg aatttggggt aattaccaat tatattgaaa tgttgctgga attaccatgg
    21841 ggtgagtata cagcagataa tttcgattta aaacatgcta aaactgtttt ggatgaagac
    21901 cattttggtt tagataaaat taaggaccgt ttaattgaat atttagccgt acttaaattg
    21961 aaaggcgatt taaaatctcc tattttatgt ttggttggtc ctccgggcgt tggtaaaact
    22021 tcgttgggcc agtctattgc aaaagcatta ggcagaaaat atgtgcgtat gagccttggg
    22081 ggtttacacg atgaaagtga aattcgcggt caccgcaaaa cctatattgg cgccatgccg
    22141 ggtcgtgttg tacagtcaat taaaagagta aaatcttcta acccggtatt tattttagat
    22201 gaaattgata aggttggaac cgattttcgt ggcgatccat caagtgcact tttagaagta
    22261 cttgacccgg aacaaaacag ctctttttat gacaattatt tagagttgga atacgacttg
    22321 tctaaaatat tatttattgc aacagcaaat aatattaata ccattcaacc tgctttgcgc
    22381 gaccgcatgg aaattattta tttaagcggt tactctacag aggaaaaagt ggaaataggt
    22441 aaacgccatt tagttccgaa acaaaaaaag gaacatggtt taaaacaacg ggatatttca
    22501 cttaccgatg atgtgttaac acaaattatc gaaaactata cacgtgaaag tggtgtgcgt
    22561 gattttgacc gccaaattgc ttctgtaatg cgccgtattg caaaatatgt ggctaccgga
    22621 gaaaagtata ataaaaccat aaaacaatcg gaagtagaag gaatacttgg aacagcaaaa
    22681 tttgataagg aaagtatatt taatactact gttccaggtg tggttgttgg attagcctgg
    22741 acgcctgtgg gtggtgatat tttgtttatt gaaacatcac ttagcaaagg aaaaggcact
    22801 ttacagctta ccggtaactt aggtgatgtt atgaaagagt cggcttcaac agctttgaca
    22861 tttataaaat cgcatgccga tgaattagga ttgtcgcctg atatttttga acaaaccgcc
    22921 attcacgtgc acgtgcctga aggcgccatt ccgaaagatg gtcctagtgc aggtatcaca
    22981 atgctcacct cactcgcatc ggcattcact ggcagaaaag tgaaatcgta tttagccatg
    23041 acaggcgaaa taactttgag aggtaaagta ttaccggttg gcggcattaa agaaaaggtg
    23101 ctggctgcaa aacgctccgg agtg
//
LOCUS       DAORKW010000010        22940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_15_length_22940_cov_5.859384, whole genome shotgun sequence.
ACCESSION   DAORKW010000010 DAORKW010000000
VERSION     DAORKW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 22940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22940
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15_length_22940_cov_5.859384"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLB46_00985"
     CDS             complement(<1..315)
                     /locus_tag="PLB46_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF75114.1"
                     /translation="MQNLQYPVGKFIAPTAFSTTLLDNYISTIQSFPDKIIAATSNLT
                     EQQLDTPYRPDGWTVRQVVHHCADSHMNSIIRLKLALTEDKPTIKAYNESAWAKMADS
                     ITF"
     gene            complement(376..2592)
                     /locus_tag="PLB46_00990"
     CDS             complement(376..2592)
                     /locus_tag="PLB46_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75115.1"
                     /translation="MKNTLSLLLLLTAYNTAFSQSDQWISWQTHNNVDDIAFDCNYTW
                     ITTSSAIIAYNNDTGEQVFFSESNSGISGSINAIEVDEDGNKWISSTTGLFFLKNNII
                     KRIESDVIDTALAHTYFLYNLKIDGKGNIWFSNANNTIYKYNPTTNNCDVFTLPVSSN
                     KIQLAINDVGNVYALVTSNFFGYFIYTLNENITEVATFEQLPFNAIYPSSFVIDSENN
                     YWIVIPAEVTYGKDDMQILTGGIIVYNSAGWYKFDVQEIQAVEDIAGYQSAFKLLSGN
                     IILQDQIAISAVAPNHTYYARFDKLNQTLNFELFLDTINRYTIEGVDLENNIYYQHWD
                     LIKLNASGEQSSIPLYQIDQALSSDIYIGINNAFWIYEMGNLGLLQSGHFTNYNDKIT
                     GYTDNRLLNVLCDSINTWFIFPELIVKFDGVTWQYYSLAAFGLDTLVSEIEFVQMDKQ
                     NNFWLLGNNSLLKQNGGSWDVMHVFNESELYFYDFIIADTTAYIIGIDSMFVYDQNTW
                     QSKLWGEDGLPEMDGNVICVAKENGTMFLSTNDTIYSCSGLDVNVFLDVKTSILTYSQ
                     QTQTLSWMLGDTLYQLDQFSVLSSWPVIYNNYFQTIGTDDLGNILILSEFYDPILMFN
                     PDGLIGYPDAILNTVDVPLANCGYYINEMQGLEIKVFPNPSTAYIDVYFSLLNSGDIN
                     ISIYDITGQLMASENKTSVDKGVVLLNYDVSLFTPGVYFIQVNANNQTSSLSFIRL"
     gene            2719..4524
                     /locus_tag="PLB46_00995"
     CDS             2719..4524
                     /locus_tag="PLB46_00995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF75116.1"
                     /translation="MPDKTSLYAPLLVETAWEVCNQVGGIYTVVRSKAPEIVDKWAGN
                     YCMVGPLLHSKLPAEFEEGFDYDDAFGQAVLEMRKLGFEIHYGRWLTDGKPKVVLMPP
                     TQVYDKLKDIRTTLLHDHKINIFNPDDLLQQVIAFGYLVTQFLTTLEQLQKGNPIIAH
                     FHEWMTGMPIGAIKKEGLRIKTVFTTHATLLGRYIAGNDDNFYRNLSNYDWLQMAQKF
                     YIEPQTRLERMAANDCDTFTTVSDITGKECEFLLGRKPDMILPNGISLPRFLATHELQ
                     NLHGQFRNKIDEFVMSHFFHSYTFDLDKTVYFFTSGRYEYHNKGYDLTLEALKRLNDR
                     MKKENTDKTVVMFFITKRPFETINPLVLHARAAMDDIRSTCEEMLKQIREKLFKAAAT
                     NVDPNVPELNDFIDDYWKFRYRKQIQSWKSDFWPIVVTHNLKNDAGDEILGYLRSHDL
                     INKPGDKVKMVYHPDFVAATSPLFGMEYPQFVRGCHLGLFPSYYEPWGYTPLECVARG
                     VPAITSDLSGFGNYCQTKFRNLDEKGIYIVKRKTKTFDESADELCEMMYNFVQLSRRE
                     RIILRNTVESYAENFDWSNLASNYEKAYQLVLQGK"
     gene            complement(4673..5437)
                     /locus_tag="PLB46_01000"
     CDS             complement(4673..5437)
                     /locus_tag="PLB46_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75117.1"
                     /translation="MKKIYSLLSILFVAGAVNAQVNVTYMVDITDYLAAGNTLLPGGI
                     RIGGDFGALAAENAGTPMVSWAPADPTCALTDMGSNVWSITVTYPTASIGMTQPYKFV
                     NDNWGTNEGTDAANTIATDSCGTDDGGGNINRSLVIPDADVMLQYCWDRCFKCDGSDP
                     IVLGVENVNLVSGVVVSPNPVSTTAAISFNLQAAAEVSVSIINMMGQEVYTVANANEV
                     AGNHAYSVDVTTLPAGNYVYRVIAGDAVTSGNIVKL"
     gene            complement(5888..6799)
                     /locus_tag="PLB46_01005"
     CDS             complement(5888..6799)
                     /locus_tag="PLB46_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75118.1"
                     /translation="MKTLYTFLFLILGFGAASAQSYVFDSVGVKSIEIGTVVIKNDTW
                     VIKVDKANFTQYYLPVNLPEKYCINNQEVVFEGAIGRIPTNVKMVGTPLKLNMIRVLY
                     RTKPNPNNGETLSEEKVSQTLPDPDSVGFISNANGKIIAIGDVFLIEQEINGETKRYV
                     PDFLPQDFLVANTAITFSATILKSDPNVRMMGTPIKIKDIKSESTEQFDAQKIQDAVK
                     DLFPFDSVGFLPETKGTINLIAGTFVIEVDNGRNDITRYLPVMLPDDFKKEGLLVMVS
                     GTIGKIPANVRMVGTPLTLATIGEIID"
     gene            6899..8374
                     /locus_tag="PLB46_01010"
     CDS             6899..8374
                     /locus_tag="PLB46_01010"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75119.1"
                     /translation="MNRRLTLLPILFLSAFNLYGQDWALFPQGQKSTFLYSTYINLIY
                     GLNPHIVITDGDTTTSYFAYENIDFNEYTCQETVDAYFPIDGYNMLPGLDSIVYANGH
                     TSLYGYGIDFLMTFNNNANTGDTFHLLSPDNEDIYMKCFLAMEDDVFGVMDSVKKFKF
                     YKESGTTLLNDFTIVLSKHYGLLSFLDFYDLANFDGYGTPRYYSLLGLKTPSNTIGYQ
                     YKDWTNFFPYSTGDIRIWRADSYNPDPFNYIAFTQYFKDSITSVNYYPDSIVYTYNRT
                     KKDTDDVLTYIFDIHHNFKKEDFTQFITAYPSKPVLSNCNEFNPWYSEEFKVWNNAGT
                     SYILDGGDTITTYRWTSGAGYVDTTICEVFEIYDVGNGITLNDQHGVIFMSQSINDFS
                     YTWALIGGTLNGEPFGETDFGHLTITAQVKQPLHIYPNPVSDNISITGLTQQNERYVI
                     YTITGICVQQGFPESEIDVRNLQSGMYFIQIGNSTASFIKQ"
     gene            complement(8376..9107)
                     /locus_tag="PLB46_01015"
     CDS             complement(8376..9107)
                     /locus_tag="PLB46_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF75120.1"
                     /translation="MKYSCLLVDDENLALRLLEDYVNKIPQLTIVGKCQNALSALQIL
                     QTQQIDILFLDIQMPDLTGIELIKILKNKPEIILTTAYAEHALEGYQLDVTDYLLKPI
                     PFERFVQAVNKAMEYRNLRNAGVSNIQLTSAPVSTAAPALDYMFVKSDYKQIKVPYAD
                     ILYIEGLREYVSIYTKEKRIVTLETLKNLELILPAQQFLRIHKSYIINTSKVEALNGN
                     MVEMGKIKVPIGKNYREATLSVLKQ"
     gene            complement(9104..10123)
                     /locus_tag="PLB46_01020"
     CDS             complement(9104..10123)
                     /locus_tag="PLB46_01020"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF75121.1"
                     /translation="MSYVLKKYEWLWHCLFWALFVLLMTLIFARFLGFEQALLRNFAT
                     CLVLAPLVYINLYVLVDRLLFKEKYFEYAGIIVLLITVTSPLRALIDKQFSFNENNPL
                     VLYSVAHYGTIVLSSVIMLGVTTTLKLLEAYYEKKTIAADLTRLKLEAELKFLKAQVN
                     PHFLFNTLNNVYALTHLEGKAAAPQILKLSALMRYMLYESNENLVPLSKELEYIHNYL
                     ELQQLKNEVTHRVTFVVNGNPDKISVAPLLFIPFFENAFKHGDVLDNKDGFLTATINI
                     ESNSLHFSIENNIADQAARKDAVGGIGLENVRKRLILLYPDKHILEVQTINNKFVVHL
                     TIELN"
     gene            10341..11174
                     /locus_tag="PLB46_01025"
     CDS             10341..11174
                     /locus_tag="PLB46_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75122.1"
                     /translation="MKTMKIQSLALVGLISLSVVSCKRADDLLDIEADPGSAKMVAET
                     TKAYDDALTMAEEATIQYLDDSTFTEYAIGNCVVITFDATNTQITFDLGTSGCVGLDG
                     RERKGKIIVDYDGVPREPGSSLHITLDDYSVDGYGISGTLTYNAINYNADGNLEFSYM
                     VENGVYTEPDGDEITLTCNRTVTWTAGAGSENLYDDVFETTGSFAGSVDGEVYSGTTT
                     SPVVFKSACWTDGIYYPVSGELNLDFPDNISRAINYGDGACDKDIVITIGKKDYPYTL
                     P"
     gene            11251..12060
                     /locus_tag="PLB46_01030"
     CDS             11251..12060
                     /locus_tag="PLB46_01030"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRF75123.1"
                     /translation="MFSQWDIQLVWVYSFLMLLTRYFLIAGAAYWCIYFIFNKRLSVK
                     KIQQRFAPNNKIINEIIASVFSFFIFAAFMTLIVYLQEIGFTKIYTDFSQYSWWYAAL
                     SFVALLFIHDTYFYWMHRLMHLPILFKHIHLHHHKSVTPTPWTAFSFHPVEAILEFAF
                     IIPVVFILPLHPVVLMIFAFSMTAMNVLGHIGYELFPKNFLQQPLGKIFNTTTHHDMH
                     HHYTKCNYGLYFNFWDLIMRTNHKKYQETFEQTASKIAPKQTANKQQLQHH"
     gene            complement(12057..13397)
                     /locus_tag="PLB46_01035"
     CDS             complement(12057..13397)
                     /locus_tag="PLB46_01035"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF75124.1"
                     /translation="MQELFVSNQSPLRIMEFRYFLSMRVFITFAFLIQYVVAGWEIYS
                     ITKDPLSLGLIGLAEAIPAIGIALYAGHLADISNKRNLLIAALFGMLISSIGLTLFTS
                     DYMRQINTDATTVLLMYVFIFTTGLARGFYGPTTTSIIPQIVLPQKTFTQNNTNKIQA
                     LDSIDEEELMDGESQKIQNQQLIKASTMNTTIWQMSAIIGPALGGFIYAGFGMTISMS
                     IVVICIITGIILFTQVASKPAVQKNNTEPILDRLKEGIRFVFQNKIILTALSLDMFSV
                     LFGGAVALLPVFAEDILSCGPQGLGILRAMPSLGAVITISYISWKKIPGKIGHTLLWA
                     VAGFGLTIILFGLSTNFYLSLVLLFLNGAFDSVSVVIRSNIIQILTPDSMRGRVAAVN
                     TMFIGSSNELGAFESGLAARLMGTVPSVVFGGTVTLLIVGFTAVKAKAIKAFSY"
     gene            13466..13918
                     /locus_tag="PLB46_01040"
     CDS             13466..13918
                     /locus_tag="PLB46_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRF75125.1"
                     /translation="MDIDPKKLRFVNNLALLTLGILMILGAGLIWGAFTEASQIGNNE
                     QPASQEIDSGSIKPVYTGKGKELFQANCQKCHLLDKKMTGPGLANVRNRWNDTSDLYS
                     WIKNSQAFLQTGDKYAIDLSRNYDNAVMPAFPNLTDDDIYEILNYLDY"
     gene            complement(13929..14255)
                     /locus_tag="PLB46_01045"
     CDS             complement(13929..14255)
                     /locus_tag="PLB46_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRF75126.1"
                     /translation="MTIQEAQDTIDRWINTTGVRYFNELTNMAMLTEEVGEVARIIAR
                     KYGEQSFKESDKDKQLSDELADVLFVLICIANQTGVNLTEALEKNLDKKTQRDSTRHQ
                     ENEKLK"
     gene            complement(14293..16620)
                     /locus_tag="PLB46_01050"
     CDS             complement(14293..16620)
                     /locus_tag="PLB46_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF75127.1"
                     /translation="MMKRFTLFLTGLFCLCILKSQNVAIGQWTSHLPYSGTVMVADAG
                     DKVYAANTRSVFSYDKTDNSYTKLSTAAGFSDIGIKGINYNKSNKTLVIAYTNSNLDF
                     LFDNEIYNYPFIQSSNISGDKNIYSASFYGDTVILSCGFGLVLFDIEARESPATFFFT
                     DTAEATIQVNQCTVYADSFFAATENGLYQGALSESNLQDFSKWNKTSGTNNLPEGAVS
                     FVTVYNNMLFAQVADTLYQYNEGAWENYFYDTIWQTNDVRTVNNNLLITQVFGTNSPA
                     DSCRVIKVDSDGTLTEIVNDTEFNYLGQSDLDEEGNIWIADAFRGLIKYNNGAYSSYL
                     PNGPFSDKVFDMKSINNELFVAPGEINASWNYAYNRDGFFLYSYGSWQAFNEFSNGLF
                     DSVFDLLAVDVDITNNIVYFGSYGGGLIEFRRNTNEMVLYKQGYLQMVAGDTNSYRIG
                     GLALDDANNLWMSNYAATDCIVVKKADGTWKSFNPEMPADIGNQVGAIAIDDFQTKWI
                     QLPRGNGILVFNDNGTIDDESDDLVKVLGAGSGNGNLHVNYINAIAVDKQNEIWVGTS
                     EGITIFYNPSEIFTGTAAGDATQPLVNLGGYYEQLLRNDIVNTIAVDGANRKWVGTNS
                     GAFLISADGTEQLLYFNADNSPLISNTVLNIEIDPVTGDVYFGTDKGIIAYRSTATEG
                     VAEINDVSVFPNPVRENYNGTIAINGLTPDADVNITDMAGRVIYKTTALGGQAVWDGK
                     GYEGNRAATGVYLVYSSNEDGSQTYVAKILMISGK"
     gene            16825..19254
                     /locus_tag="PLB46_01055"
     CDS             16825..19254
                     /locus_tag="PLB46_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRF75128.1"
                     /translation="MTYETTRVKLSYEFYKTVPEDNAAYQDYEDFKAIFGEEGNVLFL
                     GIQSERFFELDYFNDWCALEKSIKEIEDVESTLSVPSTYNLVKNISERRFDIQPLVVE
                     QPKTQAELDSIKNDFLRLPFYKDRIYSPDSNTYVMLIAIDREVLNSPRRISLINELKE
                     KTEAFGAEHQTDIKYSGLPYIRYYQLSTITREVKLFLILAVIITIIVLYILFRSWDAV
                     VFPLLIIGSGVVISTGFMALLGYEFTVLTSLVPSLLIVMGIPNCVYLLNKYHHEVRRN
                     GNKIKSLSLTVEKLGFATFITNMTKAVGFVVFCFTNVRILEEFGLIATIGVLATFIIS
                     LVGIPVVYSFLPRPKAKQTEYLESKQLKFILGKLELWTSNHRRQIFISAIIIIIISTF
                     GASRLKSKGYILDEVKETTEVYQDMKFFERVFHGVMPFEVMVTKKQVVTFVQDTIINE
                     HISVVDTDTIINYDTLIVKRPDTSGTSVMGMNTLQKIAQLQDTLATYPQLSTSLSILD
                     GLKFARQAYYNGSQRYYSLPNFNNLTTNDLKVKDYLENSGQKGVLENRFVDSKGEIAR
                     ISVQVADIGSDSMPKLMADIQPKIDAIFDKENYDVRVTGTSIVALSGYQYLIDGLFES
                     LLIALIVISLILLYQFRSLRMLAISFLPNLIPLFITGAIMGFLNIAIKPSTVLIFSIA
                     YGMCVDFTCYFVAKYKHDLIRNSFNIPKTVMSSLQEAGVSMIYTSLILFCGFFIFVFS
                     KFGGTVNMGLLTSITLLVALFNNLLLLPALILLFEKGLNKSNSKLRARAAEKANRPFT
                     DLINNDTKDIK"
     gene            19458..22766
                     /gene="ileS"
                     /locus_tag="PLB46_01060"
     CDS             19458..22766
                     /gene="ileS"
                     /locus_tag="PLB46_01060"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRF75129.1"
                     /translation="MSTKKYQEFKQLNLPEFEKGVLEYWKQNDVFKKSVAERNENNPF
                     VFYEGPPSANGTPGIHHVLSRALKDLFCRYKTMQGFRVERKSGWDTHGLPVELSVEKL
                     LGITKEDIGKKITVEEYNERCKLQVMQFKNQWEELTDIMGYWVDMENPYITYDKNFIE
                     SLWYILKTLYDKNLLYKGYTIQPYSPAAGTGLSSHELNQPGTYKPLKDTTIVAQFKVI
                     KDNHAAHPKLFAGHDEVFFLAWTTTPWTLPSNCALAVGKDITYQQIETYNQYTGKHIS
                     VVLAQDRVAAYFKEENKTHTLDPENKSSWKIVSEFKGKDLAGIQYEQLLPYQQPTTGD
                     AFKVIVGDFVTTEDGTGIVHIAPSFGADDFRVAKQNNIGTLTLVDKEGKFVDGVGPFS
                     GMFVKNYKDDEKWENPDVLIAIHLKETNRAFKVEKYEHNYPHCWRTDKPIIYYPLDSW
                     FIRTTAAKERLIELNKTINWKPQSTGEGRFGQWLENLVDWNLSRSRYWGTPLPIWRTE
                     DGVEEICIGSFEQLQEEINKAKDLNPHLPADFDARIVLDPHRPYVDAIVLRSASGKPM
                     QRESDLIDVWFDSGAMPFAQLHFPFENKEVFAKNFPADLIAEGVDQTRGWFFTLHVIA
                     TLMNDSVAYKNVISNGLVLDKNGNKMSKRLGNGVDPFTTIYKYGADATRWYMITNAQP
                     WDNLKFDNEGIVEVQRKFFGTLYNTYSFFALYANIDGFDFSQEEIPMQQRAELDRWII
                     SLLNSLIKECESSLNDYEPTRAGRAIQDFVNDHLSNWYVRLSRRRFWKSDDSADKSAA
                     YQTLYTCLQTISQLMAPIAPFMSEWLYRNLNEVTGKNSSDSVHLTLFPKAEEKLIDTE
                     LEASMALAQEISSTVLSLRKKENIKVRQPLSKIMIPVLNAQVQRQIEHVGELIKSEVN
                     IKTIEFIAEDSGVITKKIKPNFKALGPKLGAEMKTIAAQISAFTQQQIAELEKSGNYA
                     IETANKSFVISLEDVEILSEDIPGWLVANAGHVTVALDVTITPVLKEEGHAREFVSQL
                     QRMRKEMNLEITDRIGVDFEAVDMELSGALLNYKQYICAEILADTLEQVASLPESEAI
                     SVNDISIKVLIKKN"
BASE COUNT         7425 a         4144 c         4239 g         7132 t
ORIGIN      
        1 aaaagtaata ctgtctgcca tttttgccca agccgattca ttataggctt ttattgtggg
       61 tttatcttct gttaaggcaa gttttagtcg aataatacta ttcatatgac tgtcggcgca
      121 atgatgcact acttgtctta ccgtccaacc atcgggcctg tatggtgtat ctaattgttg
      181 ttcggttaaa tttgaagttg cagcaattat tttatcggga aacgattgaa tggtggagat
      241 atagttgtcc agtaaggtag tagaaaaagc agttggagca ataaattttc caaccgggta
      301 ttgtaggttt tgcattttat tgaatttgaa atggaataca tttcaatgcg gaaattagtt
      361 cattaaaatg tataattata aacgaataaa cgacaacgat gatgtttgat tattcgcatt
      421 tacctgtata aaatatacgc cgggggtaaa aagacttaca tcataattta acagcactac
      481 acctttgtca acagatgttt tattttcgct tgccattaac tgcccagtaa tatcataaat
      541 actgatattg atgtcgccgg agtttaataa actaaaatag acatctatgt atgctgttga
      601 cggattcgga aacactttaa tttctaaacc ttgcatttcg tttatgtaat agccacaatt
      661 tgctaaaggg acatcaactg tatttaaaat tgcatcggga tatccaatta aaccatcggg
      721 attaaacatt aaaattggat cgtaaaactc agacaagatt aaaatattac ctaggtcatc
      781 agtaccaatt gtctgaaaat aattattata aataaccggc caactactca gtacactaaa
      841 ttggtctaat tgatataatg tgtctccaag catccagctt aatgtttgtg tttgttgact
      901 ataagttaaa atgcttgttt taacatcaag gaatacgttt acatccagac ctgaacagct
      961 gtaaattgta tcgttcgttg ataaaaacat agtgccattt tctttagcaa cacatataac
     1021 attgccatcc atttccggca aaccatcctc tccccacaac ttcgactgcc atgtgttttg
     1081 atcataaaca aacatgctgt caatgccaat aatgtatgca gttgtgtcag ctatgataaa
     1141 atcataaaaa taaagttcac tctcattaaa aacatgcatt acatcccatg aacccccatt
     1201 ttgttttaat aacgaattat tacccaacaa ccaaaaatta ttttgtttat ccatttgcac
     1261 aaattcaatt tctgatacta atgtatctag tccaaaagct gccaatgaat aatattgcca
     1321 tgtcacccca tcgaacttga caattaattc aggaaagata aaccaggtat ttattgaatc
     1381 gcaaagtaca ttaagtaaac gattatctgt atagccggtt attttatcgt tataatttgt
     1441 aaaatgtcca ctttgtagca accccagatt acccatttca taaatccaaa aggcattgtt
     1501 aataccaata taaatatcgg aactcaatgc ttgatcaatt tggtaaagcg gaatggagct
     1561 ttgttcgcct gatgcattta attttattaa atcccaatgc tggtaatata tattattttc
     1621 taaatctacc ccttctattg tataacggtt tatagtatct aaaaacaatt caaaatttaa
     1681 agtttgattt aacttgtcaa aacgcgcata atatgtgtgg tttggagcaa ctgctgaaat
     1741 ggcgatttga tcttgtaaaa taatattgcc ggatagtaat ttaaaggcac tttggtagcc
     1801 ggctatatct tcaacggctt gtatttcctg aacatcaaat ttataccaac cagcactatt
     1861 atacacgata attccgcccg ttaaaatttg catatcatcc tttccatagg tgacttctgc
     1921 gggaattact atccaataat tattttcact atcaataaca aacgatgatg gataaattgc
     1981 attaaaagga agttgctcaa acgtagcaac ttcagtaata ttttcattaa gggtataaat
     2041 aaaatagcca aaaaaattac ttgttaccag cgcgtaaaca ttgccaacat catttattgc
     2101 aagttggatt ttattacttg atacgggtaa tgtaaaaaca tcacaattgt ttgtcgtggg
     2161 attatattta tagatggtat tattagcgtt actaaaccaa atattacctt ttccatcaat
     2221 tttgagatta tataggaagt atgtatgtgc taaggcggta tcaataacat ctgattcgat
     2281 tctttttata atattatttt ttaggaaaaa taatcctgtt gtggaagaaa tccatttgtt
     2341 tccatcctca tcaacttcaa ttgcattaat actaccactt atgcccgaat tactttcact
     2401 aaaaaagacc tgttcacctg tgtcgttatt ataggcaata attgcagaac tggttgttat
     2461 ccaggtgtaa ttgcaatcaa atgctatatc atctacgtta ttatgtgttt gccatgaaat
     2521 ccattgatca gattggctaa atgccgtgtt gtatgccgtt agcaataata ggagggacag
     2581 ggtgtttttc ataatgtgtg ttactataac gctaaaaatg gtcgaaaaat atgaatatga
     2641 taagaattga ctctttttac ccaaaatttt aaccaatgcg tatcttagtg tcttaagtaa
     2701 aaaaaacagg aaagcggtat gccggataaa acgagtttat atgcgccact attggtggaa
     2761 acagcctggg aggtttgtaa tcaggtagga ggaatttata cagtggtaag gagtaaagct
     2821 cctgaaatag tggataaatg ggccggcaat tactgtatgg ttgggccttt gctgcatagc
     2881 aagctgcctg ctgagtttga agagggtttc gattatgatg atgcttttgg gcaggctgtg
     2941 ttggagatgc gaaaattggg ttttgaaata cattacggcc gctggctaac agacggtaag
     3001 cccaaagtgg tattaatgcc gccaacacag gtgtacgata agttgaaaga tattcgaacc
     3061 acattactgc acgaccacaa aatcaatatt tttaaccctg atgatttatt gcaacaggtt
     3121 attgcttttg gttatttggt tacacagttt ttaactacgc tcgagcagtt acaaaaaggc
     3181 aatccaataa ttgcgcattt ccacgaatgg atgacaggta tgccaattgg agcaattaaa
     3241 aaagaaggat tgcgcataaa aaccgtattt accacacatg ctactttgtt gggaagatat
     3301 attgccggta acgacgataa tttttatcgc aacttaagca attacgattg gctgcaaatg
     3361 gctcaaaaat tttatatcga gccacaaacc agactggaac gtatggctgc taacgattgt
     3421 gatacgttta ctactgtaag tgatataaca ggaaaagaat gcgaattttt attaggcaga
     3481 aaaccggata tgattttacc aaatggtatc agcttgccgc gatttttagc tacacatgaa
     3541 ttacaaaacc tgcacggtca gttccgaaat aagattgatg aatttgtgat gtcgcacttt
     3601 tttcattctt atactttcga tttagataaa acagtttatt ttttcacttc aggcagatat
     3661 gaatatcaca acaaaggata tgatttaacg ctggaggcat taaagcgatt aaacgaccgc
     3721 atgaaaaaag agaatacaga taaaacagta gtcatgtttt ttattaccaa gcggccattt
     3781 gaaacaatta atccgctagt tttgcatgca cgcgcagcaa tggatgatat tcgttcaaca
     3841 tgtgaagaga tgttgaaaca aatacgcgaa aaattattta aagctgcggc aacaaatgtg
     3901 gatcccaatg ttccggaatt aaatgatttt attgacgatt attggaaatt tcgatatcgc
     3961 aaacaaatac aaagctggaa gagcgacttt tggccaattg tagttacgca taatttaaaa
     4021 aatgatgcag gtgacgaaat tttaggctac ctgcgctctc acgatttaat caataaaccc
     4081 ggtgataaag taaaaatggt atatcatcct gattttgttg cggctacgtc accattattt
     4141 ggaatggagt atccgcaatt tgtaagagga tgccacctgg gtttgtttcc aagttattat
     4201 gaaccgtggg gttatacacc acttgagtgc gttgcacgcg gggttccggc cattaccagc
     4261 gacttgagcg gattcggaaa ttattgccaa actaaattcc gcaacctcga cgaaaaaggc
     4321 atttatattg taaaacgcaa aacaaaaacc tttgacgaaa gtgcagatga attatgtgaa
     4381 atgatgtaca attttgtgca gctaagccga cgtgaacgta ttattttaag aaatacggtt
     4441 gagagttatg ccgaaaattt cgactggagt aatctggcat ctaattatga aaaagcatat
     4501 caattggtgt tgcagggaaa ataaatatca ctaaattaat tcgaattaaa agtagcttaa
     4561 atttatacaa ccatttatat taattgcgcg ctaaattttt ttcaatagat agcaaacaaa
     4621 aaaccccgac gaattaacgc cggggttttt tatgtagtaa ttagttattt aattacaatt
     4681 taacgatgtt accagaagta actgcatcac ctgcaattac acgataaaca tagttaccag
     4741 caggaagtgt agtaacatca acgctataag catggttacc ggcaacttca tttgcattag
     4801 caacagtgta aacttcctga cccatcatgt tgatgatgct tacagaaact tcagcagcag
     4861 cttgtaaatt aaagctgata gcagcagttg tagaaacagg gttaggagaa accacaacac
     4921 ctgaaactaa gtttacattt tcaacaccta atacgatagg atcgctacca tcgcatttga
     4981 aacatctgtc ccagcaatac tgtaacataa catcagcatc agggataact aatgaacggt
     5041 tgatgtttcc accaccatca tcagttccac aactgtcagt tgcaattgta ttagcagcat
     5101 ctgttccttc gtttgttccc cagttgtcgt ttacaaattt gtaaggctga gtcataccaa
     5161 tagaagcagt tggataagta acagtgatag accacacgtt gctacccatg tctgttaaag
     5221 cacaagttgg atcagcaggt gcccaagata ccataggagt tccagcgttt tcagcagcaa
     5281 gtgcaccaaa atcgccacca attctgatac caccaggaag cagtgtatta cctgcagcta
     5341 agtagtctgt aatgtcaacc atataggtaa catttacctg tgcgttaact gcaccggcta
     5401 caaacaaaat tgaaagtaaa gagtaaattt ttttcataac taaactgttt tttaagctta
     5461 aaaattaaaa tatcagccta aagttaaaga ttatttcaat aaaaacgccc taatttttaa
     5521 caagaaattg actgtagaat ataatattga acagttgctc ttgttgagtt ttcacccctc
     5581 ttatcaggtg taaatttttc gacgttaaaa aacattttca gaaggctaat aaaattgaat
     5641 gtggcggtat aattggctga tagccgataa cttaggagtt ataaagaggt gggtaatagt
     5701 tagttgcaaa accagtgttt aaggtaatta attggtaaaa cctgaaattg tagtcattac
     5761 aataagtaag gatttttaaa aatgcggcaa gttttgagag gcgtaaaccc cgtgcttata
     5821 gcagactccg gcttggtttg gggttcttcc gcttagctta aataaccagg aagaacacaa
     5881 ttgccaacta gtcaattatt tcgccaatgg tagcaagcgt taaaggagtg cctaccatac
     5941 gcacattggc cggtatttta cctatggtgc ctgaaaccat aacgagtaat ccttctttct
     6001 taaaatcatc gggcaacatt acaggcaaat aacgggtaat gtcatttctg ccattatcaa
     6061 cctcaatcac aaaggttccg gcaataaggt taattgtacc ttttgtttca ggtaaaaaac
     6121 caactgaatc aaatggaaat aaatctttca cggcatcttg aattttttgt gcatcaaatt
     6181 gttcggttga ttcagatttt atgtctttaa ttttaattgg agtgcccatc atccgcacat
     6241 tagggtcgct ttttaaaatg gtagcactaa atgttatagc ggtattggca actaaaaaat
     6301 cttgcggtaa aaaatcggga acataccgtt tcgtttcacc gtttatttcc tgctcaatta
     6361 agaacacatc gccaatggca ataatttttc cgtttgcatt actaataaaa ccgacagaat
     6421 cgggatctgg caaggtttga cttacttttt cttccgacaa tgtttcgcca ttattcggat
     6481 tgggtttggt gcggtataac actctaatca tattgagttt aagcggagtg ccaaccattt
     6541 taacattagt tggaatacgc ccgatagcac cttcaaatac cacttcctga ttgttaatac
     6601 aatatttttc aggcaaatta accggcaaat agtattgggt aaaattcgct ttgtccacct
     6661 taattaccca agtatcgttt tttatcacta ctgtccctat ttctatactt ttaacgccaa
     6721 cggaatcaaa aacataagat tgcgccgaag cagcgccaaa acctagaatc aggaataaaa
     6781 aggtgtacaa agttttcata cgcattatgt ggctaaaatt ataccaaatt gttttgaatc
     6841 tgcacttttt aactccagaa tgagattgaa aaatactttt ttgtaacttt attcaaccat
     6901 gaaccgaaga ttaacacttt taccgatatt gtttttgagt gcatttaacc tgtatggtca
     6961 ggattgggca ctatttccgc aaggccaaaa aagtacattt ttatattcta cctatataaa
     7021 tcttatttac gggttaaacc cgcatatcgt aattacggat ggtgatacca ctacttctta
     7081 ttttgcctat gaaaacatag attttaatga atatacctgt caggaaaccg ttgatgcgta
     7141 tttccctata gacgggtata atatgcttcc aggacttgac tccattgtgt atgcgaacgg
     7201 gcacacatcg ttgtatggtt atggaataga ttttttaatg acatttaaca acaatgccaa
     7261 taccggagat acatttcatt tgctatcacc ggataatgaa gatatttata tgaaatgttt
     7321 tttggctatg gaagatgatg tttttggtgt aatggacagc gttaaaaaat ttaaatttta
     7381 taaagaaagc ggcacaacac tgctcaatga ttttactatt gtgttgagca aacattatgg
     7441 tttattaagt tttttggatt tttatgattt agccaatttt gatggttatg ggactccgcg
     7501 atattattct ttgttaggat taaaaacacc ttcaaatact attggatatc aatataaaga
     7561 ttggacgaat tttttccctt attcaacagg agatatcaga atttggcggg cagattcata
     7621 taatccggac ccgtttaatt acatcgcttt tacgcagtat tttaaagatt ccattacaag
     7681 cgtaaattat tatcctgata gtattgtata tacctataat cggacaaaaa aagatacaga
     7741 cgatgtatta acttatattt tcgatataca tcataatttt aaaaaggaag actttacaca
     7801 gtttattact gcctaccctt ctaaaccggt attaagtaat tgtaatgagt ttaatccctg
     7861 gtatagcgaa gaatttaagg tatggaataa tgctggaacc agttatattt tagatggtgg
     7921 tgataccatc actacttaca ggtggacatc aggagcaggt tatgttgaca ccacaatatg
     7981 cgaagttttt gaaatttatg atgtgggaaa tggtattact ttaaacgacc aacatggggt
     8041 gatttttatg agtcaatcga taaatgattt tagttatacc tgggcactta ttggtggtac
     8101 attaaatggc gaaccattcg gcgaaaccga tttcgggcat ttaacaataa cagctcaggt
     8161 aaaacaacca ttacatattt atcctaatcc ggtatccgat aatatttcta ttacaggatt
     8221 aacgcaacaa aatgagcggt atgttattta tacaattacc ggaatttgcg tgcaacaagg
     8281 ttttccggaa tctgaaatag atgtacgtaa tttacaaagc gggatgtact ttattcaaat
     8341 tggcaattca accgcttcgt ttattaaaca gtaatttatt gttttaatac gcttaaagtt
     8401 gcttcgcgat aattttttcc gataggcact tttatttttc ccatttccac catattgcca
     8461 tttaatgctt ccacttttga agtattaata atgtatgatt tatgaatgcg caaaaattgc
     8521 tgagccggta atatcaattc cagatttttt aaagtttcga gggtgacaat tcttttttct
     8581 ttggtgtaaa tactcacata ttcgcgcaac ccttcaatgt aaagtatatc ggcatacggt
     8641 accttgattt gtttgtaatc ggatttaaca aacatataat ctaaagcagg tgcagcagtt
     8701 gaaacagggg cacttgttag ctggatattg gatacgccag catttctcag gtttcggtat
     8761 tccattgctt tattgacagc ctgcacaaat cgttcaaacg gaattggttt taatagatag
     8821 tcggttacat caagctgata tccttccaat gcatgttcgg cataggcggt ggtgagaatt
     8881 atttccggtt tgttttttaa aattttaatg agttcaatac ctgttaaatc cggcatttga
     8941 atatcgagaa acaaaatgtc aatttgttgt gtctgtaata tttgtaacgc agacaatgca
     9001 ttttggcatt tgccaacaat agttaattgc ggaattttat ttacataatc ttccaacaag
     9061 cgcaaggcta aattttcatc atcaactaac aaacaggagt atttcaattg agctcgattg
     9121 taaggtgaac aacaaattta ttatttatag tttgaacttc gagtatatgt ttatccggat
     9181 acaacaaaat taaacgcttg cgaacattct ccaatccaat gccaccaaca gcatctttac
     9241 gggctgcctg gtccgcgata ttgttttcga tactaaaatg taagctgtta gattctatgt
     9301 ttattgtagc cgttaaaaac ccatccttat tgtctaatac atcgccatgt ttaaacgcat
     9361 tttcaaaaaa cggtataaac aataaaggcg caacactaat tttatccgga ttgccattta
     9421 caacaaatgt tacacgatgt gttacttcgt ttttaagctg ttgtaattcc agatagttgt
     9481 gtatatattc caactcctta gaaagtggca ccaggttttc gttgctttca tataacatat
     9541 agcgcatgag tgcacttaat tttaatattt gtggtgcagc agctttgcct tctaaatgtg
     9601 tgagtgcata aacgttattt aaagtattaa ataaaaaatg gggatttacc tgcgccttta
     9661 aaaattttaa ttccgcttct aattttaatc tcgttaaatc tgccgcaatt gtttttttct
     9721 cgtaataagc ttccaataat tttaaagttg tagtaacgcc caacataatt acagaactaa
     9781 gcacaatagt accataatgt gcaaccgaat acaggacaag cggattattc tcattaaaac
     9841 taaattgttt atctattaat gccctgagtg gtgaagtaac tgtgattaat aagacaataa
     9901 tgcccgcata ttcaaaatat ttttctttaa acagcaggcg gtctaccaaa acatataaat
     9961 taatataaac aagcggcgca agcactaaac aagtggcaaa attgcgcaac aacgcctgtt
    10021 caaaacccag gaagcgggca aatatgagtg tcattaataa aacaaacaat gcccaaaaca
    10081 aacaatgcca taaccactca tattttttaa gcacataact catgctacaa acataaccaa
    10141 ttcggcaggt ttgtattatt tttttctacc aacaatagtt ttttttcaac caacggaaac
    10201 aatttactac caacatatcg aattaacctc taatggatga tttattgatg ttggtgtagt
    10261 tgctatgcta ttagattgaa tttattttcc gcgggcaaat aagcacaaca tctttgcctc
    10321 atcaacaaac aaaaaaaatt atgaaaacga tgaaaattca atcactcgcc ttagttggcc
    10381 ttatttcatt atcagttgtt tcatgtaaac gcgctgatga ccttttagac attgaagcag
    10441 atccaggaag tgcaaaaatg gttgcagaaa ctactaaagc atacgatgat gcgcttacaa
    10501 tggcagaaga agctactatt caatatttag acgattcaac atttacggaa tatgccattg
    10561 gaaattgtgt ggtaattact ttcgatgcaa caaataccca aatcactttc gatttaggca
    10621 cttccggctg tgttggttta gatggtcgtg aacgcaaagg aaaaattatt gtcgattatg
    10681 atggcgtgcc tcgcgaacct ggctcaagtt tgcatatcac cctcgacgat tattcggtag
    10741 atggttatgg cattagcgga acattaacgt ataatgccat caactacaat gccgatggca
    10801 atctggaatt ttcatatatg gttgaaaatg gtgtttacac agaacctgac ggagatgaaa
    10861 taacacttac ctgcaaccgc actgttacct ggacagcagg agccggaagc gaaaatttat
    10921 atgatgatgt attcgaaact acaggcagct ttgccggcag cgtagatggt gaagtatata
    10981 gcggcaccac aacctcaccg gtagtgttta aatctgcttg ttggaccgat ggaatttatt
    11041 atccggtaag tggtgaattg aacctcgatt ttccggataa tattagcaga gccattaatt
    11101 acggtgatgg agcatgtgat aaagatatcg ttataactat aggtaaaaaa gattacccat
    11161 atacactccc ttgatgattg gttttaagct gacggcgtgg gcaaatcgga ttgccccgcc
    11221 atttttttta atctaaaaat aatactgttt atgttttcgc aatgggatat tcaactcgtt
    11281 tgggtttatt catttttaat gttgctaacc cgatattttt taattgccgg cgctgcttat
    11341 tggtgtattt attttatatt taataagcga ctatcagtta aaaaaattca acaacgtttt
    11401 gcgccgaata ataaaataat aaatgaaata atcgcatctg tattttcatt ttttattttc
    11461 gcggctttca tgacattaat tgtttattta caagagattg ggttcaccaa aatttatacg
    11521 gatttttctc agtatagctg gtggtatgcg gctttgagct ttgttgcact tttatttatt
    11581 cacgacactt atttttattg gatgcacagg ctcatgcatt tgcccatttt attcaaacat
    11641 atacatctgc accaccataa atcggtaaca ccaacgccct ggacagcatt ttcattccat
    11701 ccggttgaag ctattcttga atttgctttt atcattcccg tggtgtttat tttacccttg
    11761 catccggttg tgcttatgat atttgcattt agcatgacag ccatgaatgt gttgggtcat
    11821 atcggttatg agttattccc caaaaatttt ctacaacaac ctttaggaaa aatatttaac
    11881 acaacaacgc atcacgatat gcatcatcat tatactaaat gtaattatgg attatatttt
    11941 aatttttggg atttaattat gcgtactaac cacaaaaagt atcaggaaac gtttgaacaa
    12001 acggcgagta aaattgctcc gaaacaaacc gcaaacaagc agcaattgca acatcactaa
    12061 taactgaaag ctttaatggc tttggcctta acggcggtaa atcctactat aagcagtgta
    12121 acggttcctc caaaaaccac tgatggaacg gtccccatta aacgggcagc caggccgctt
    12181 tcaaaagccc ccaactcgtt actgctacca ataaacatag tgttaactgc tgctactctt
    12241 cctcgcattg aatcaggtgt taatatttga ataatattac tgcgaattac cacactcaca
    12301 ctgtcgaaag caccattcaa aaataacagc accaacgata aataaaaatt ggtagacaat
    12361 ccgaataaaa taatagtaag accaaatccg gcaacggccc acaacagggt atgtccaatt
    12421 tttcccggaa tttttttcca tgaaatataa ctaatagtaa taacggctcc caacgatggc
    12481 attgctctta aaatgcccaa accttgtggg ccacatgata aaatatcttc tgcaaaaacc
    12541 ggaagcaaag caactgcacc gccgaataaa acggaaaaca tatctaaact taatgcagtt
    12601 aaaataattt tattttggaa tacaaaacga ataccttctt ttaatctgtc taaaattggt
    12661 tctgtattat ttttttgcac tgccggtttt gaagctacct gggtaaataa aataatacct
    12721 gtaataatac aaattaccac aattgacatt gaaatggtca taccaaaccc agcataaata
    12781 aatccaccta atgcaggtcc tataattgcg ctcatttgcc agatagtagt attcatggta
    12841 ctggctttta tgagttgctg attttgaatt ttttgtgatt cgccatccat taattcttct
    12901 tcatcaatac tatcgagcgc ttgtatttta ttggtattat tttgagtgaa tgttttttgt
    12961 ggaagtacaa tttgcggaat aatagaagtt gttgttggac cgtaaaaacc tcttgcaagt
    13021 ccggttgtaa aaatgaacac gtacatcaat aaaacagttg tggcatcggt atttatctgc
    13081 cgcatataat cggatgtaaa cagggtgagc ccgattgatg aaatcaacat accaaataat
    13141 gcggcaatta ataaattccg cttattgctg atatctgcca aatggcctgc ataaagcgca
    13201 ataccaatag caggaatggc ttctgccaaa ccaattaaac ccaaagagag ggggtctttt
    13261 gtgatggaat aaatttccca tcctgcaaca acatattgta ttaaaaatgc gaacgtgata
    13321 aaaacccgca tactcagaaa gtagcggaat tccataatgc gtaaaggtga ttggttgctc
    13381 acaaataatt cctgcacaaa attaacgcgc taaacattaa cactgtaatt ttggatataa
    13441 taattgcatt cctaaaagtt atagtatgga tatagaccct aaaaaacttc ggtttgtcaa
    13501 caatttagcg ctacttaccc tcggcatttt gatgatatta ggcgcggggc tcatttgggg
    13561 cgcttttaca gaggcatcac aaataggcaa taacgaacag cctgcttcac aagaaattga
    13621 tagtggttcg ataaaaccgg tgtataccgg aaaagggaag gagttattcc aagccaactg
    13681 tcaaaaatgc catttattgg ataaaaaaat gaccggaccc ggattagcga atgtaagaaa
    13741 ccgttggaat gatacaagtg atttgtacag ctggattaaa aattcacaag cctttttaca
    13801 aacaggcgat aaatatgcta tcgatttatc cagaaattat gataatgcag taatgcccgc
    13861 atttcctaat ttaacagatg atgatattta tgaaatatta aattaccttg attattaata
    13921 gtcggttttt atttcagctt ctcattttcc tgatgccgag ttgaatcgcg ttgagttttt
    13981 ttatcgagat ttttttcaag agcttcggta agattaacac cggtttgatt ggcgatacat
    14041 attaatacaa ataatacatc agcaagttcg tcgctgagtt gtttgtcttt atcgctttct
    14101 ttaaacgatt gttcaccata ttttctggca ataattctgg ccacttcacc cacttcttcg
    14161 gtgagcattg ccatgttggt aagttcatta aaataacgaa caccggtagt gtttatccag
    14221 cggtctatcg tgtcttgcgc ctcctgaatt gtcatagtac aaatgtatgc attaactaca
    14281 ggaacaaaat atttatttcc cgctaatcat taatatttta gccacataag tttgtgaacc
    14341 atcttcattt gagctgtaaa ctaaatacac acctgtcgca gcgcggttgc cttcgtaacc
    14401 tttaccatcc caaacggctt gtccgcccaa tgcagttgtt ttataaataa cacggcctgc
    14461 catgtctgta atatttacat cagcatccgg agttaatcca ttgatagcaa tggtgccatt
    14521 gtaattttct cgaacaggat ttggaaatac agatacatca ttaatttctg ctacaccttc
    14581 tgttgcagtg gagcgataag caataatacc tttatcggtg ccaaaatata catcgccggt
    14641 aacgggatct atttcgatat ttaatactgt atttgaaata agcggagaat tgtcggcatt
    14701 aaaatacagt aattgttcag tgccatctgc actaattaaa aatgcgccgc tatttgtacc
    14761 aacccatttt ctgtttgcgc catcaacggc aatggtattt acaatgtcgt tgcgcaataa
    14821 ttgttcgtaa tatccaccga gattaaccaa aggttgtgtg gcatctccgg ctgctgttcc
    14881 ggtaaaaatt tcagatgggt tataaaatat ggttatgcct tcactggtgc ctacccaaat
    14941 ttcattttgt ttgtcaacgg caatggcatt aatataattc acatgtaaat taccatttcc
    15001 ggaaccggcg ccaagtactt tcactaaatc atcactttca tcatcaatag tgccattatc
    15061 attaaaaact aaaataccat ttccgcgtgg taattgaatc catttagtct gaaaatcatc
    15121 aatcgcaatt gctccaacct gattaccaat gtcagcaggc atttcaggat taaacgattt
    15181 ccatgtacca tctgcttttt ttactacaat acaatcggta gcagcataat tcgacatcca
    15241 taaattattg gcatcatcta atgctaatcc gccaatacga taagaattag tgtcacccgc
    15301 aaccatttgt aaataaccct gtttgtataa taccatttcg ttagtattgc gcctgaattc
    15361 tatcaacccg ccaccgtacg aaccaaaata tacaatgtta ttggttatat caacatctac
    15421 tgcaagtaaa tcaaaaacac tatcgaacaa accatttgaa aattcattaa atgcttgcca
    15481 actaccataa ctgtatagaa aaaatccatc gcggttgtag gcataattcc acgaagcatt
    15541 tatttcaccg ggagcaacaa ataattcgtt gttgatactt ttcatatcaa aaactttatc
    15601 actaaaagga ccattgggca aataagaact ataggcgccg ttgttatatt taattaatcc
    15661 acgaaatgca tctgcaatcc aaatattacc ttcttcatct aaatcgcttt ggcccaaata
    15721 attaaattcg gtgtcgttga caatttcagt taatgttccg tcactatcta cctttattac
    15781 gcgacaagag tcggccggtg aatttgttcc aaatacctga gtgattaata aattattatt
    15841 tacagtgcgc acatcatttg tttgccaaat ggtatcgtaa aaataatttt cccatgcacc
    15901 ttcattgtat tgatataatg tatcggcaac ttgtgcaaat agcatattat tataaacggt
    15961 aacaaaactc acagcgcctt cgggcaaatt gttggttccg cttgttttat tccattttga
    16021 aaaatcctgt aagttagatt ctgataacgc cccttgatac aatccatttt cagtagcagc
    16081 aaaaaaagaa tcggcataca cggtgcattg atttacttga atggttgcct cagccgtatc
    16141 cgtaaaaaag aatgtagccg gactctcacg agcttcaata tcaaataata ccaatccaaa
    16201 accacaactt aaaataacag tgtccccata aaaggaggcg ctataaatat ttttatcgcc
    16261 gcttatattg ctggattgaa taaacgggta gttataaatt tcattatcga acaggaagtc
    16321 gaggttgcta ttggtatatg caattaccaa tgttttgttt gatttgttat agttaatgcc
    16381 tttaatacca atatctgaaa aaccggcagc cgtgctcagt ttagtataag agttgtcggt
    16441 tttgtcgtaa ctaaacactg aacgggtatt agccgcatac actttatcgc cggcatctgc
    16501 caccataacc gttccgctat aaggcaggtg tgaggtccat tggccaatgg caacattttg
    16561 gcttttgagt atacaaaggc agaacaggcc ggttaaaaac aaggtaaaac gcttcatcat
    16621 cgcagagcaa atttccgaaa tagaaccata taacgcataa aacagctaaa tatttcagca
    16681 aatccttcat caatacagta aacagcggct gttaatttag tatctttgcc atccttttta
    16741 aatatggttt ggagggcact aggtaggata attttaagga atcgctgggc gtttttggcg
    16801 gcatgggtgg ttataactgc cttcatgacc tatgaaacta cccgtgtaaa gcttagttat
    16861 gagttttaca aaacagtgcc tgaggacaat gctgcttatc aggactacga agattttaag
    16921 gcaatttttg gagaagaagg taatgtcctg tttttaggca ttcaaagtga gcgttttttt
    16981 gaactggatt actttaacga ctggtgcgca ctcgaaaaat cgataaaaga aatcgaggat
    17041 gtggaaagca ccctttccgt gcccagcacc tataacttag ttaaaaacat ttcggagcgc
    17101 aggttcgaca ttcagccctt ggtagttgaa cagcctaaaa cccaggcaga gcttgattct
    17161 atcaaaaacg actttttacg acttcccttc tataaagacc gaatttatag ccccgactcc
    17221 aatacttatg tgatgctgat tgccatcgac cgcgaagtac tgaactcgcc aagaaggatt
    17281 tcgttaatca atgaattaaa agagaaaaca gaagcatttg gtgctgagca tcaaacagat
    17341 ataaaatatt ccggattacc atacatcagg tattaccaac tttcaaccat cactcgtgag
    17401 gttaagttat tcctcatttt agcggtaata attaccatta tcgtgttgta tatcctgttc
    17461 cgaagctggg atgcggtggt gtttccgttg ctaattattg gttcgggagt ggttatttcc
    17521 accgggttta tggccttgtt ggggtatgaa ttcacggtat taaccagttt agtgccgagt
    17581 ttactcattg taatgggtat tccgaattgc gtgtatctgt taaataaata ccatcacgaa
    17641 gttcgccgaa acggcaataa aatcaaatcg ctgagcctta ctgtcgaaaa actcgggttt
    17701 gccactttta ttaccaatat gaccaaagcg gttgggtttg tggtattttg ttttaccaat
    17761 gtgcgcatat tagaagaatt cggacttatt gccacaattg gtgtgctggc aacctttatt
    17821 atttcattgg taggtattcc ggtagtgtat agttttttac ctcgacctaa agcaaaacaa
    17881 acagaatatc ttgaaagcaa acagctgaaa tttattttag gtaaacttga actttggaca
    17941 agcaatcacc gccgtcagat ttttatttct gctatcatca ttattattat ttcaactttt
    18001 ggtgcctcgc gattaaagtc aaaaggatat atacttgatg aggtaaaaga aactacggaa
    18061 gtgtatcagg acatgaaatt ttttgaacgc gtatttcatg gtgtgatgcc tttcgaagta
    18121 atggtaacaa aaaaacaagt ggtaacattt gttcaggata ctattattaa cgaacatatt
    18181 tcagttgttg atacagatac tatcattaat tacgatacac tcattgtaaa acggcccgat
    18241 acatccggca ccagtgtaat gggtatgaac accctgcaaa aaattgcaca gctgcaggat
    18301 actttagcaa catatccaca actttctact tcgttatcta ttcttgacgg attaaaattt
    18361 gctcgtcagg catattataa tggcagtcag cgatattatt cattacccaa ttttaataac
    18421 ctgaccacca acgatttaaa agtaaaagac tatctcgaaa actcaggcca gaagggcgtt
    18481 ttggagaata gatttgttga ttcaaaaggt gaaattgcgc gtatcagtgt tcaggtagcc
    18541 gatatcggaa gcgatagtat gcctaaatta atggcagata ttcaacctaa aatagatgcc
    18601 attttcgaca aagaaaatta tgatgtacgg gtaacaggaa ccagtattgt tgcattgtcc
    18661 ggttatcaat atttaattga cggattgttt gaaagtttat taattgcatt aattgttatt
    18721 tcgctgatat tgttatatca attccgcagt ttgcgtatgc tggcaatttc atttttaccg
    18781 aatttaattc cgttatttat taccggtgct attatgggct ttttaaatat tgccataaaa
    18841 ccttctacgg ttttaatatt tagtatagct tatggtatgt gtgtagactt tacctgttat
    18901 tttgttgcaa aatataaaca cgatttaatt cgcaacagtt ttaatattcc aaaaacagta
    18961 atgagtagtt tgcaggaagc cggggttagt atgatttata cctcattaat tttattctgc
    19021 ggatttttca tatttgtgtt cagcaaattt ggtggcactg taaatatggg attactcact
    19081 tcaattactt tattggttgc cctgtttaac aatttattat tgttgcctgc actcattcta
    19141 ctatttgaaa aagggttaaa taaatcgaac agtaagttgc gagcccgtgc tgctgaaaaa
    19201 gcaaacaggc catttaccga cttaatcaat aacgacacca aggatatcaa ataaccggcc
    19261 tagtttgtta ttcaggcttc ttggaaagtt caccggattc atcgttttta ccctttagta
    19321 ttgccgaata catggcaaaa tacccgaatt tgtattcacg cctacctaaa acctgcgcaa
    19381 aatctactct tttgcctcga aacaattaat tttgcacgct taatcatcaa aggttaatag
    19441 ttgatgattt ttactatatg tctacaaaaa agtatcaaga atttaagcag ttgaaccttc
    19501 cggagtttga gaagggggtg ttggagtatt ggaagcaaaa cgatgtgttc aaaaaaagtg
    19561 tggctgaacg caacgaaaac aatccctttg tattttatga aggtccgcca agcgctaacg
    19621 gcacaccggg tattcaccat gtgttgtcgc gtgcattgaa ggatttattc tgccgttata
    19681 aaaccatgca gggattccgt gttgagcgca aaagcggatg ggatacacat ggtttacctg
    19741 ttgaactcag cgtggaaaaa ttattgggta ttaccaaaga ggacatcggc aaaaaaatta
    19801 cagttgagga atataacgag cgttgtaagt tgcaggtaat gcaatttaaa aatcagtggg
    19861 aagagctcac cgatattatg ggatattggg tagatatgga aaacccatac attacctacg
    19921 acaaaaattt tattgaatcg ttgtggtata ttttgaaaac actttacgat aaaaatttat
    19981 tgtataaagg gtataccatt caaccctatt ctccggcggc aggaaccgga ttaagttcgc
    20041 atgaattaaa tcagccggga acgtataagc cattaaaaga tactacaatt gttgcgcagt
    20101 ttaaagtgat taaagataat cacgcagcgc atccgaaatt atttgcagga catgatgaag
    20161 tattttttct ggcttggaca actacaccat ggacattacc aagcaattgt gcattagcag
    20221 ttggaaaaga tataacgtac cagcaaattg aaacgtataa tcaatataca ggtaaacata
    20281 tttcggttgt tttggcacag gacagagttg ctgcttattt taaagaagaa aataaaacac
    20341 atacacttga ccctgaaaat aaatcaagct ggaaaattgt ttctgaattt aaaggaaaag
    20401 atttagcggg tattcagtac gaacaattat taccttatca gcaaccaaca acaggggatg
    20461 catttaaagt aattgttggg gattttgtta ctactgaaga tggaacagga atagttcaca
    20521 ttgcaccaag ttttggagca gatgacttcc gcgtagcgaa acaaaataat attggcacat
    20581 taacattagt tgataaagaa ggaaaatttg ttgacggtgt tggtcccttc agtggaatgt
    20641 ttgtaaaaaa ttataaggat gatgaaaaat gggaaaatcc tgatgtgtta attgccatcc
    20701 atttaaaaga aacaaaccgc gcatttaaag tagaaaaata cgaacacaat tatccgcatt
    20761 gctggagaac cgataagccg attatttatt atccgcttga cagttggttt ataagaacta
    20821 ctgcagcaaa ggaacgatta attgaattaa ataaaaccat taactggaag ccacaaagca
    20881 ctggcgaagg gcgttttggg cagtggttag aaaatttagt agactggaat ttatcgcgtt
    20941 cacggtattg gggaacacca ttacctattt ggcgaactga agacggggtt gaagaaattt
    21001 gtattggttc atttgaacaa ttacaggaag aaattaataa agcaaaagat ttaaatccgc
    21061 atttacctgc agattttgat gcgcgaatag ttttagatcc acacaggcca tatgttgatg
    21121 caattgtatt acgcagtgcc tcaggcaaac caatgcaacg cgaaagcgat ttaattgatg
    21181 tgtggtttga ttcgggtgct atgccatttg cacaattaca ttttccgttt gaaaataaag
    21241 aagtttttgc aaaaaatttc cctgccgatt taattgccga aggtgttgac caaacacgtg
    21301 gatggttttt tacattacat gttattgcca cattaatgaa cgatagcgtt gcttataaaa
    21361 atgtaatcag caacggatta gttttggata aaaacggtaa taaaatgtcg aaacgtttgg
    21421 gcaatggtgt tgatccattt accaccattt ataaatatgg agcagatgcc actcgttggt
    21481 acatgattac caatgctcaa ccatgggata atttaaaatt tgataacgaa ggcattgtag
    21541 aagtacaacg taaatttttc ggaacgttat ataataccta ttcatttttt gctttatatg
    21601 cgaatattga tggatttgat ttttcgcagg aagaaattcc tatgcaacaa cgcgctgaat
    21661 tagaccgttg gattatttcg ttattaaatt cattaattaa agaatgcgaa agtagtttaa
    21721 atgattatga accaacaaga gccggaagag caattcagga ttttgtcaac gaccatttga
    21781 gcaactggta tgtgcgttta agtcgccgac gtttttggaa aagtgatgac agtgctgata
    21841 aatccgctgc ttatcaaaca ttatatacct gtttacaaac tatttcacaa ttaatggcgc
    21901 caattgcacc gtttatgagt gagtggttgt atcgtaattt aaacgaagta accgggaaaa
    21961 acagttctga ttcggtgcat ttaaccttat ttccaaaggc agaggagaag ttaattgata
    22021 ctgagttgga agcaagtatg gctttagctc aggaaatttc ttcaaccgtt ttgtcgctcc
    22081 gcaaaaaaga gaacatcaaa gtgcgtcagc ctttatcaaa aatcatgatt ccggttttga
    22141 atgcccaggt tcagcgtcaa attgaacacg ttggagaatt aattaaatct gaagtaaata
    22201 ttaagacaat tgagtttatt gctgaggaca gtggggtaat taccaagaag ataaagccga
    22261 atttcaaagc ccttggtcca aaactcggcg ccgaaatgaa gacaatcgcc gcccaaatca
    22321 gtgcatttac ccaacaacag attgcagagt tggaaaaaag cggtaactat gccattgaaa
    22381 cggcaaataa aagttttgtt atttcgttag aagatgttga aattttgagt gaagacatcc
    22441 ccggatggct ggtggcgaat gcgggacacg ttacggtggc attagatgtt accataacac
    22501 cggtgctgaa ggaagaaggc catgcaagag agtttgtaag ccagcttcaa cgcatgagaa
    22561 aggaaatgaa cctggaaatt accgaccgta taggagttga ttttgaggca gttgatatgg
    22621 aactttcagg ggcattgctt aattataagc agtatatttg cgccgaaatt ttggctgaca
    22681 ctttagaaca ggttgcgagc ctgccggagt cggaagcaat ttcagtaaac gacatatcaa
    22741 tcaaggtcct gatcaaaaaa aattagcatc atggcaaaaa aaccagctaa aaaggcagca
    22801 aaaactgcta agcctaaagc aaaagtgaac acaaaagcta aggttgctgc taaagcacca
    22861 gtaaaaaaag ctgccccaaa agcagctaaa aaagcccctg caaaaccggt aaaaaaggct
    22921 acagcagcta aacctgccgc
//
LOCUS       DAORKW010000011        20462 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_18_length_20462_cov_5.545989, whole genome shotgun sequence.
ACCESSION   DAORKW010000011 DAORKW010000000
VERSION     DAORKW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20462
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18_length_20462_cov_5.545989"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..851)
                     /locus_tag="PLB46_01065"
     CDS             complement(105..851)
                     /locus_tag="PLB46_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF75130.1"
                     /translation="MKQFSLLCYFLISALLLSAQSIPNDTTANKWSLFKFKKHKQETT
                     YTPYCSSCEITQFKPETYFNDDDSDGVYNVFDLQLNTPALAPVDSNGVAVDSDGDGML
                     DFEDAEPCLNNHFLSNSYADNNSTKHVNHRTYETDNVMLPYVFFQFNTSEIIQSQYNL
                     VPIKFIADFLKNQNGTLAYVTGHASQRGIEASNVDLKFNRAVVIINKLIEENIQPNQL
                     AIYFPLSSSYLFIENDEVLNSFVSCSIHHH"
     gene            complement(954..2564)
                     /locus_tag="PLB46_01070"
     CDS             complement(954..2564)
                     /locus_tag="PLB46_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF75131.1"
                     /translation="MISRKLTLAVLMLFSIITFTQAQTASGGSSFPPKPANKWQIGLN
                     LGLPVIQGDLRSEPFSSTHGPAFGVGLNIRKGLGYVTSLRLHGMYANTYGQDFIQGSY
                     AYNKTLNGAFSNSPVTNYSEGADYTGNVWVPNYKTQIGHGSLDLLVNLNNINFHKADP
                     KFLVYAFAGIGGFIFNTSYDALDASGNPYDFQTILSGFGSQNTSEKDRIDAIHGVLDG
                     EYETQAEVANPASRDAGEVRATWVRNPSLSGGLGLSYKIGSSLEIGLETRATHYFDDL
                     ADGQRWELDGDYTDHYDMFQYTSLNVGISLGGDESVDPLWMVNPLTYLYDKVNEIDPD
                     NLLKDSDNDGVIDRLDKEPNTAEGTPVDTHGVSLDSDKDGCPDSEDPEPFSSPVLPME
                     NCQNVHLTQDWVEKYVDERIKAQGGTPGAASDWILPYIFFDLNKSEIRPDAVPSLNQV
                     AEIMNRYTKLNIEVVGHTDTRAGEDYNMKLSENRARTAITYLTGKGVAESRLKIVYKG
                     ESDVMIKDANTEAEHQMNRRVEFHIIKN"
     gene            complement(2914..4776)
                     /locus_tag="PLB46_01075"
     CDS             complement(2914..4776)
                     /locus_tag="PLB46_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF75132.1"
                     /translation="MRKFLLLLLLFITLIQVNGQGIVAYPDTTICSSQPVWLHADIDG
                     SYGTLTYEYASIPFAPEPVGGTVHNMTDDTHVGPLSIGFDFCFFGEVYDKFYIASNGW
                     ISFKLPNTSMDLNWTPNGPIPDASSDVPKAAIFAIWTDWDSGSCTNCIHHELVGVAPN
                     RKLIISYTNIPLFLCTTFEGVFQIVLHETTNKIENHLTDVEVCAGWDLGIGTQGIINE
                     DGTEAFTIAGRNATNWSASNESYAWLPSAITWYETVSGAFVGTGDSIEVAPTVTTSYT
                     AEVLLCDGTTYTDEVIVTVATPYVVNVDIQNISCFGNTNAWIDVDVTGNTNPVTYEWS
                     DGSVTDSIFNLGPGTYTITIQEEDGCAYFQDIVITEPTELLLDTVSTSDVICFGGNEG
                     SVILNADGGVSPYLFSFDGVTYQTDSNFLTMEAGIYTFTVKDAYGCITTFDGVVISQP
                     EPTLVEAGPNTVIEYGSSTPLNATTSVSPIVDILWSPAEGLSCTDCLQPIAAPTFNTT
                     YYVVITDENGCLAIDSVTVWVDIDFNVPNAFTPNDDGLNDEFNIQTEMLISYYIAIYN
                     RWGDLVFTSDDINDGWDGTQNGVLQEIGTYMYVIESVTTLNTPITKTGTLTLLR"
     gene            complement(4815..5483)
                     /locus_tag="PLB46_01080"
     CDS             complement(4815..5483)
                     /locus_tag="PLB46_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HRF75133.1"
                     /translation="MSQIPKKSLQFLKQLAANNNKPWFDAHRNDYDEAKADFINFIAE
                     IIKGVSAFDNTIVGLEAKKTVFRINRDVRFSKDKSPYKSNLGATLSAGAKNMQTAGYY
                     FQIQPGNKSFAGVGSYMPIPENLAKIRQEIDYNYKAFHKILTSAAFKKQFDGLDEIEV
                     LKTVPKGYTADNPALPYLKHKSFVVTRYFTDAEVQDPAFSKTLIHCLKTAYPLVKFIN
                     SAIE"
     gene            6326..7675
                     /locus_tag="PLB46_01085"
     CDS             6326..7675
                     /locus_tag="PLB46_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75134.1"
                     /translation="MEAQQTTMPLLSKVYNYSTFSKSQVLINGELEKNSSSYSKGHPE
                     YGILPFNAGCDDCSEIIDQRTINTRYYVGNDPKERAVYAQTSLNDLHYKNEQGDWITI
                     DYRLHPTKNENIYSALNQEQPTSINLNDYYTSIQLTTSTKLVFNGKWSLSGSKKPEMS
                     KLMNPTNHSVGEDGAILFNAWDGIDIRHTFTKGSIKTDFVVNNLSAIDPTSAYSIFKE
                     TIQLPDGFSIIRDIYEGEESINGRWVGDLILVDAFGNEYARYDHVFTYMENPIDEEMA
                     ALNLIGEYAIEQVGNLVTLSLYIDNSWLTDPARTFPIVIDPTVSGTLATIAGTPLGSR
                     FWPEYCVHTMMVSTPANATLTTANVALDVDAKAGGCTPSGNCQRNFTQVYISTSCGVD
                     PIGAPGIIYTCVGGGTCLLPGHLVLPTTSMPHLVLDCFTPSCASFTIPFNVHLQRLA"
     gene            7700..8554
                     /locus_tag="PLB46_01090"
     CDS             7700..8554
                     /locus_tag="PLB46_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75135.1"
                     /translation="MPCIQLDLFEVTLKGKTVEATATAAGGTSYTILDCTDQSDWLAP
                     DEDIYGVPAYTYSWSPIGATDDSIYVTFPLGTTSYTLTITDACGNIATDVVTVTNNCI
                     LLPLPLTEFSGYHQNDFNLLNWSTQSEIGTNYIIERSVTGNDFIPIGTVESKSNTGFQ
                     TYSFSDLSPDNQIVYYRLKQINTKNEVLYSDVIAIKTAYKPGYEIISTNFDAENNMLQ
                     LSINSEKDLTADMIIYDLAGKIVFTNSIALATGTTVVKSTLPELAKGAYVVHIVVEDQ
                     NLESKFVN"
     gene            8691..10625
                     /locus_tag="PLB46_01095"
     CDS             8691..10625
                     /locus_tag="PLB46_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRF75136.1"
                     /translation="MISVQNLSIHFGGIFLFEDVSFALKENDRVGLVGKNGAGKSTLM
                     KIMSGLQKPDSGQVMLPKDSLIGFLHQDLSKMKGKTVFEETQTAFNEALQLDKRMHEI
                     EHELTVRTDYESDSYHDIINEIQEISHRLEIINVATIDKQIELVLMGLGFMREDFDKP
                     VETFSGGWQMRIELAKILLSKPELVLLDEPTNHLDIESIQWLETYLETYPGAIMIVSH
                     DRTFLDNVTKRTIEIVSQKIYDYPANYSTFVELRKERIDQQRAAQKNQEKYIEHTEKL
                     IDKFRAKANKAKFAQSLIKKLDKIEEIEVDDEETATIHFYFPKPQRSGKTVVTGKKVS
                     KTYDVKLILDEIDFDIQRGDKIAFVGKNGEGKSTLSKIIAGIEKYQGELELGHNVHLG
                     YYAQNQAETLSGDDTVFDTIDKVATGEMRSHIRTLLGAFLFGGEASFKKVKVLSGGEK
                     SRLAMCKLLLEPYNLLVLDEPTNHLDMRSKDVLKNALNNFEGTVIVVSHDRDFLKGIT
                     NRVFEFKDKKIKEHIGDIYDYLEAKKISSLAELELAPKPVKQKKETTTISPTKENHED
                     KKNREREVNRIKKEIEKTEKEIAAIETEIVQLEKQMNDPGFYEKPNTTEIFNKYNNLK
                     ESHTQKMSGWESLTEQLETI"
     gene            10698..11012
                     /locus_tag="PLB46_01100"
     CDS             10698..11012
                     /locus_tag="PLB46_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF75137.1"
                     /translation="MAYSKKHEFDAESIRIADIAKALSHPARIEILKVLSNGQCVCGD
                     IVDQLPLSQSTVSQHLKELKAAGLIKGEIDGPRVCYCLNEGVCNQALGFFRQLFREIK
                     CC"
     gene            11014..11454
                     /gene="arsN2"
                     /locus_tag="PLB46_01105"
     CDS             11014..11454
                     /gene="arsN2"
                     /locus_tag="PLB46_01105"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HRF75138.1"
                     /translation="MYTFRTATIDDLTAIQTLLIQHQLPVDGLTQHIQHFEVVMANNH
                     LIGVAGVEQYFPFALIRSVAIDAQYQHQHIGKKLIENLLSKLNKAGFTTYYLLTETAP
                     TFFSKLNFHIIQRSEVPLVIQQTQEFATICPASAIVMTKNNLSL"
     gene            11465..12304
                     /locus_tag="PLB46_01110"
     CDS             11465..12304
                     /locus_tag="PLB46_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HRF75139.1"
                     /translation="MQNDQEVKDLVKERYSEIALQSKTTNASSCCGAGGCSTIDLEIM
                     SENYTSLEGYFADADLGLGCGIPTEFAQIKKGDTVVDLGSGAGNDAFVARSIVGEDGR
                     VIGVDMTETMIELSRKNAAKLNLQNVEFRLGDIENIPITSNKADVVVSNCVLNLVPNK
                     VKAFSEMYRIIKPGGHFSVSDIVLVGELPASILKEAEMYAGCVSGAIQKDEYLNIIAQ
                     NGFKQVSIKKEKEIILPDDILSNYLNEEQMTAFKNSGVGIFSITVFGEKPKDCGCGTD
                     CCS"
     gene            12701..13285
                     /locus_tag="PLB46_01115"
     CDS             12701..13285
                     /locus_tag="PLB46_01115"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRF75140.1"
                     /translation="MQKSIVLTLSIAMLSLFGACQNSSQDAIKEKRKEEMTNKENRVS
                     PPATATATIGTNTVTVNYSSPAVKGRPIWGALVPYGEVWRTGANEATTVTFTQDVTIQ
                     GQTLTKDTYSYFTIPTDSVWTVIFNVNETQWGAFKYDQSEDALRVQVKPVMVETNTEN
                     LSFSVVPDATPNSGIIRLNWEKLQLDCPFTNVAN"
     gene            complement(13358..13765)
                     /locus_tag="PLB46_01120"
     CDS             complement(13358..13765)
                     /locus_tag="PLB46_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75141.1"
                     /translation="MKLLARCYLQVILLYFIAFGFYACNETELPDDNFNASQNFNRTI
                     IFDVNRIYDISPLMDSTVQDVKIDIYLSYEELAYDYYPEATRTTDENGHAEINGLDKD
                     YYYIKATHPVLGILIDSVTTPANTTSFVMLTYY"
     gene            complement(13868..14503)
                     /locus_tag="PLB46_01125"
     CDS             complement(13868..14503)
                     /locus_tag="PLB46_01125"
                     /inference="COORDINATES: protein motif:HMM:NF017966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromophore lyase CpcT/CpeT"
                     /protein_id="HRF75142.1"
                     /translation="MKKLVFIFLFLVSFQSQAQLENIYGTLLAYMTGSFSSEAQSKAD
                     SNFYDIRLHMVEIQMGNEPGNWLYVEQALGSAQDKPYRQRVYQVVEITLGIYESRVYE
                     FENPLQYAGGWNDESKLSNLTFDKLILRDGCSITLKYEHELVGDININRFVGSTGATS
                     CPSSLRGASYATSEVVITEDQLLSWDRGWDANGKQVWGAETGGYIFVKTTE"
     gene            complement(14504..16048)
                     /locus_tag="PLB46_01130"
     CDS             complement(14504..16048)
                     /locus_tag="PLB46_01130"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRF75143.1"
                     /translation="MIANDAEKFKKVISHAKEYGYIFQSSEIYDGLSAVYDYGPYGAL
                     LKNNIKEYWWKAMVQMHENIVGLDSAILMHPTTWKASGHVDAFSDPLIDNKDSKKRYR
                     ADVLIEEFLAKQDEKIDKEVEKAKKRFENFDEAMFRTTNGRVLEIAAKREAVHTRFVK
                     ALNDNNLDELKQIIIDCEIVDPISGTRNWTDVRQFNLMFSTSFGAASDSSDVVYLRPE
                     TAQGIFVNFLNVQKTTRQKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRP
                     GEEMNYYNYWKKTRMQWHRALGTDASMLQFHDHDKVAFYANAAVDIEFKFPFGFKELE
                     GIHSRTNHDLSRHTEFSGKKLQYFDPELNENYIPYVVETSIGCDRMFLAMMCNAYDEE
                     GVGEETRVVLRLPAILAPIKVAVLPLIKKDGLPEKAMEIFNTLKFNHQCYYEEKDSIG
                     KRYRRMDAIGTPYCITVDHQTLEDNTVTIRERDSMQQERIAIADVLQIVNTKVDMNTI
                     LKQLVD"
     gene            complement(16693..17589)
                     /locus_tag="PLB46_01135"
     CDS             complement(16693..17589)
                     /locus_tag="PLB46_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75144.1"
                     /translation="MKKNQNYFILNYSAFAIVYLLCKNVQAEAIYTEIDPDIILSEDG
                     DAYNLDMNNDGLLDFKLQKISGSFYSYWSENISYAYYIAISPWQSGNKMAGLKSVISP
                     SYGGFTQYFPFALNSMHLINEDLTFQNNNFEILAYIINSDDGASQALGGQWFYGLENK
                     FLGVRFKDSLDCFHYGWIRCSITSEADSLTIHDYGYETKCETGIMAGDTIGDTTTVGI
                     SELNLFAPTIYSYSGTICINIDKQLLGANYSVVNLEGKLVISGYLNELNNKFNSVVGN
                     GTYIVAINKNSYSYSKQIILVQ"
     gene            complement(17586..20147)
                     /locus_tag="PLB46_01140"
     CDS             complement(17586..20147)
                     /locus_tag="PLB46_01140"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75145.1"
                     /translation="MKNSNNKLMATAISLFLIILLTSNKLFAQYFPDEETFDTSGLIQ
                     ETLTDGLDTVWYWGIDGPTEGMNLKTEGTTYYQNSNNISQYSKVHADSTIYINLGSTQ
                     KSVNKGLYGFHIEGIYSRKVIPNDGSAVDQWQWMSELAPSSLRFPGGSSSKFMHLLDG
                     PGYGYDIEEIIRFYDRTDSVVNAPSYISIKDSIEADAGALFYLTWITAKEVDDFIGFA
                     DKWVEQNLLDSNHLYIDDFVYMVKQIEVANPGQKVDVIIDLNIMNETATRSKEIVEYL
                     RNHDTCNVNVVYVELGNEMYFSFSETMLGIYTLEDYWTYINGGITDSLDSVLIGTDVW
                     FDHDYISTFKSSPLFTCKIGLPVENLKDPTYAFREAASSGIRAANEWNDDLFEKRLEK
                     VEIEGLPGHFRKSFDAYIVHPYYDTHNWDTIALYNLDSVYECESPDTIGWRYDIHDPR
                     LEDAFDGLAKNFKKFTNTRYQESWNVHRDTLGLNLSVSSGGKEIITSEYNFKETGGDY
                     TIYERDKLGVFAQTFLTSAMLQEWWLKNLKINYNNSYREGFFKYATLHNFAGASHSAM
                     LSPAFGPELDSLNRDTIPYNISTDSLEGRNYYMKRASYFTMLMLSDIPKNGLKYLQTN
                     FTLAKNNINVQPTVFIDSEKENFYFYYSNVSADPQPYYINLTGTSGIFPPDGHVQITD
                     TVTIYCVNALQPYSTGGRGKNTLYTINKCYNDINYNVDYNIEIDTFFTLTNSPSSGSP
                     FTTVEVPAYSFGYFKVPIIADYPPYEKIERKEPHFLVFPNPSNQSISFSISGLEDATT
                     SGLEVKITTIDGKICYKNWCSMESPIDISYLSSGIYLIFVEMGNGRFFKEKIIKQ"
BASE COUNT         6711 a         3635 c         3618 g         6498 t
ORIGIN      
        1 tattttgtgg tggtgatgga gtatttagtt gtaattgggg tttgtttgtt tgaggttttt
       61 attttgccgg gaaggcgggg agaaaggaag tttcgggaag aggtttaatg atgatgtatt
      121 gagcaggata cgaagctatt tagcacttcg tcgttttcaa taaatagata gctgcttgaa
      181 agtggaaaat atatggccaa ttggtttggt tgaatatttt cttcaattaa cttattaatt
      241 attaccactg ctctattaaa ttttaaatca acattagaag cttctattcc gcgttgcgaa
      301 gcatgacctg taacataagc gagtgttcca ttttgatttt ttagaaaatc agcaataaat
      361 ttaatgggaa ccaaattata ctgactttga attatttcag atgtattaaa ttgaaaaaaa
      421 acataaggca acattacatt gtctgtttca taagttctat gatttacatg cttagttgaa
      481 ttattatccg cataagaatt actcaaaaag tggttattca aacaaggctc cgcatcttca
      541 aaatccaaca ttccatctcc atcactatca acagccacac cattggaatc aactggtgca
      601 agtgccggtg tatttaattg taagtcgaaa acattgtaaa ccccatcaga atcatcgtcg
      661 ttaaaatagg tttcgggttt aaattgggtg atttcgcaac tgctgcaata gggagtatat
      721 gttgtttcct gtttatgttt tttaaacttg aacaatgacc atttatttgc tgtggtatcg
      781 ttaggaatag attgtgctga gagcaataaa gctgaaatga ggaaataaca cagcaggctg
      841 aactgtttca tgctttacga atatactgcg aaatatacat ttagaaaact aaggtacata
      901 aaaaaagggg tggattgctc caccgctttg gctgtcatga ttatatgtat atatcagttc
      961 ttaattatat ggaattcaac acgacggttc atttgatgtt ccgcttctgt gttagcgtct
     1021 ttaatcatta catcagattc acctttgtaa acgattttca aacggctttc tgctacacct
     1081 ttacctgtta agtaagtgat tgctgttcta gcacggtttt cagataactt catgttataa
     1141 tcttcaccag cgcgagtatc agtgtgacca actacttcaa tatttaattt agtgtaacgg
     1201 ttcatgattt cagcaacttg gtttaaagaa ggtactgcat caggacggat ttcagatttg
     1261 ttaagatcga agaagatgta aggtaaaatc caatcgctag ctgcacctgg agtaccacct
     1321 tgagctttaa ttctttcgtc aacatatttt tcaacccaat cttgagttaa gtgaacgttc
     1381 tgacagtttt ccataggtaa tacaggagaa gagaatggtt ctggatcttc tgagtcagga
     1441 caaccgtctt tatcgctgtc taagctaaca ccgtgagtat caaccggagt accttcagct
     1501 gtgttaggtt ctttatctaa acgatcgata acaccatcat tatcagagtc ttttaataag
     1561 ttgtcaggat cgatttcgtt aactttatca tacaagtaag taagtgggtt aaccatccaa
     1621 agtgggtcaa ctgattcgtc gccacctaag ctgataccaa cgtttaagct tgtatattgg
     1681 aacatatcat agtggtcagt atagtcacca tctaattccc aacgctgacc atcagctaag
     1741 tcatcaaaat agtgagtagc tctggtttct aaaccaattt caagtgagct accaattttg
     1801 taagaaagac ctaaaccacc agataatgaa gggttacgta cccaagtagc acgaacttca
     1861 cccgcgtcac gtgatgcagg gttcgctacc tcagcttgag tttcgtattc gccatctaat
     1921 acaccgtgga tagcgtcgat acgatctttt tcagaggtgt tctgagaacc gaatccacta
     1981 agaattgttt ggaaatcgta aggatttccg cttgcatcta acgcatcata tgatgtgttg
     2041 aagataaaac caccaatacc agcgaaagca tacactaaaa atttagggtc agctttgtgg
     2101 aaattgatgt tattaagatt aaccaataag tctaatgacc cgtggccaat ttgagttttg
     2161 tagttaggaa cccaaacgtt accagtatag tcagcacctt ctgagtagtt tgttaccgga
     2221 ctgtttgaaa aggcaccgtt gagtgttttg ttgtaagcat atgaaccttg gatgaaatcc
     2281 tgtccataag tgtttgcata cataccgtgt aaacggagag aagttacata ccctaatcct
     2341 ttgcggatgt taagaccaac gccaaatgct gggccgtgtg tgctgctaaa aggttcagat
     2401 cttaaatcac cctggataac aggaagacct aagtttaaac caatttgcca tttgttggct
     2461 ggttttggag ggaatgatga tccaccacta gctgtttgag cctgtgtgaa ggtaataatg
     2521 ctaaaaagca tcaataccgc taacgtgagt tttctactaa tcatgacgta taaattatag
     2581 tttaaatata atcggttata taaaagattg tttatgaata tctcttttat gccgcaaagc
     2641 tataaccctt agtccgctta tgcaagtgat ttgacctcaa atcgtggaaa ttttccacaa
     2701 attcacggta atcagttaat agcgtaggtt tcaacatgat gttaaattta tatgttaaaa
     2761 cggttacaag ccttatggca ctaacattta actattagtt agagcaaagc tatacactta
     2821 accgtaaaaa ccaaaaaaaa atgaattttt ttttatgttt gatgcaacca aattaaaaat
     2881 tagagcatcg aatgcctttt acaggggtaa aaactacctg agaagtgtta gggtaccggt
     2941 tttggtaatt ggtgtattga gtgtagttac cgattcaatc acatacatat aggtgccaat
     3001 ttcctgtaaa acaccatttt gggtgccatc ccaaccatcg ttaatatcat cagaagtaaa
     3061 aacaagatct ccccacctat tgtaaatggc gatataataa gaaataagca tctctgtctg
     3121 aatattaaac tcatcattta acccatcatc attaggtgta aacgcatttg gcacattaaa
     3181 atctatatct acccaaacag taaccgaatc aatggctaaa catccatttt catctgttat
     3241 cacaacgtaa taggtagtat taaaggtggg tgccgcaatt ggttgtaaac agtcggtaca
     3301 actaagtcct tcggccgggc tccacaaaat atcaactatt gggcttaccg aggttgtcgc
     3361 attcaagggt gttgaactcc catattcaat aactgtgttt gggccggctt caacaagcgt
     3421 tggttcgggt tgactaatta caacaccatc aaatgtggtg atgcaaccgt aggcatcttt
     3481 aacagtaaat gtataaatgc cggcttccat ggtcaaaaaa ttactgtcgg tttgatatgt
     3541 aaccccatca aacgaaaaaa ggtaggggct tactccgcca tcggcattta atatgactga
     3601 cccttcattt ccaccgaaac aaatcacgtc ggatgttgac acagtatcca acaataattc
     3661 tgtaggctcg gtaattacta tatcctgaaa ataggcgcat ccgtcttctt cctgaattgt
     3721 aatggtatag gttccaggtc ccagattgaa tatactatct gtaacagagc catctgacca
     3781 ttcgtaagta accggattgg tgttgcctgt tacatctacg tcaatccacg cgttcgtgtt
     3841 gccgaaacaa gaaatatttt gaatatctac attaactaca taaggagtag ccactgtaac
     3901 tattacttca tcggtatagg tagtaccatc acataataaa acctcggctg tataacttgt
     3961 agttactgtg ggagcaactt caatactatc cccggttcca acaaatgcac ctgaaactgt
     4021 ttcgtaccaa gtaattgcgc ttggtaacca ggcataactt tcgtttgaag ccgaccaatt
     4081 ggtagcattc ctgcctgcta tggtaaatgc ttcagtgcca tcttcgttta ttatcccttg
     4141 agtaccaata cctaaatccc accctgcgca aacctcaaca tctgttaaat ggttttcaat
     4201 tttattggtg gtttcgtgca aaacaatctg aaaaacacct tcaaaagtgg tgcacaaaaa
     4261 caatggaata ttggtataac tgattattaa tttgcggttt ggtgcaacac ctaccaattc
     4321 atggtgaata cagttagtac aactacccga atcccagtct gtccaaatag caaaaatggc
     4381 agcttttggc acatcactgg aagcatcagg aattggtcca tttggtgtcc agttcaagtc
     4441 catcgatgta tttggaagtt taaaacttat ccaaccattt gaagcaatat aaaatttatc
     4501 atacacctcg ccaaaaaaac aaaagtcaaa tccaattgaa agcggtccaa catgcgtatc
     4561 gtcggtcata ttatggactg tgccaccaac gggttcaggg gcaaaaggaa tggatgcata
     4621 ttcataagtt aatgtcccgt aactaccatc tatatcggca tgtaaccata caggttgtga
     4681 gctgcaaata gttgtgtctg gataagctac tatgccctga ccatttactt gaattagcgt
     4741 gataaataat aataataaaa ggagaaattt acgcatgata cgttttctac taatgattca
     4801 tacgtcaaat atacttattc tatagcacta ttgatgaatt ttaccaatgg ataagccgtt
     4861 tttagacaat ggataagtgt tttactaaat gccgggtcct gcacttcagc atcggtaaag
     4921 taacgggtta caacaaaact tttatgtttt aaatacggta atgcaggatt gtcggcagta
     4981 tacccttttg ggactgtttt taaaacttct atctcatcaa gtccgtcaaa ttgtttttta
     5041 aacgctgccg aagtaagtat tttatgaaat gctttataat tataatcaat ttcctgtctg
     5101 attttagcca aattttcggg aataggcatg taggagccta ctccggcaaa acttttattt
     5161 ccgggttgta tttggaagta ataacctgct gtctgcatgt tttttgctcc cgcacttaat
     5221 gtggctccta aatttgattt gtaaggactt ttatctttag aaaaccgaac atcgcgatta
     5281 attcgaaaca ctgttttttt tgcttctaat ccaacaatgg tgttatcaaa tgcactaaca
     5341 cccttaatga tttctgcaat aaaattgatg aagtcagctt ttgcttcatc ataatcattc
     5401 ctatgggcat caaaccatgg tttattatta ttagctgcca gttgttttaa aaattgtaac
     5461 gattttttag gaatttgaga catgatatat tcaagtttag tatgctaaat tataattaca
     5521 gaaacggttt agtttcataa aaccaaaatt aaatatatgc aacaacatct cacaataatt
     5581 ttggtatcat ttactgaaat tcactcacaa gaaattttaa ttcaatccta ttatttcaaa
     5641 ttagattaaa ggatatggaa ccatacaatt ataaacgata aagtttttat tctttcgcgt
     5701 atttctttta tggttacgat ttactcaaaa ttccatttga caaaaccttc aagccttttt
     5761 tgcaaaaaac gtatttatta cctcaacatt tgttaaataa gcagttcttt tagttacttg
     5821 aaaagccgtt aattgaaacc tctattggcg aaaaacgcta aattattcct ttttacccca
     5881 cttttagcaa atgggtggcc cttaacagta ttggcgataa ccaggttaaa ctctttaacg
     5941 ttaatggaaa tgtaatatta tcagcggtta cctcaacaag tgttaaatca aaaaaatcgt
     6001 tataatgaaa atatttttaa tatataaagt attgataata aattagttat gattattttt
     6061 taaaaaatct gatatccaaa atcggtgatt ggacatagtg gatttagggg aataagcacc
     6121 cttataagga aaattttaat actttaagca gcatgggaca aattggattt cgctaaaata
     6181 attgccaaaa tgcgactaat taagtatttt tattggctaa ttgaaccact aattgttcaa
     6241 ttaaacacac ataaagtcgg acctttttat gaaaaagcag tatttattta ttgtttacat
     6301 gttccttttt tgctttcagc aaaatatgga agcacagcaa acaacaatgc cccttttatc
     6361 caaggtttat aattattcca cttttagtaa aagtcaggtt ttaattaatg gtgaacttga
     6421 aaaaaattca agttcatata gtaaagggca tccggaatat ggcatcttac cttttaatgc
     6481 tggttgtgac gactgtagcg agataattga ccagcgcacc ataaatacga gatattatgt
     6541 tgggaatgac ccaaaagaaa gggctgttta tgcacaaaca tcattaaacg atttacatta
     6601 taaaaatgag caaggtgatt ggattacaat tgattacaga ttacacccga caaaaaatga
     6661 aaatatttac agcgcattaa atcaggaaca accaacttca ataaatttaa acgactacta
     6721 tacatccatt caattaacaa cttcaactaa gttggtattt aatggaaaat ggtctttaag
     6781 tggaagtaaa aaacctgaaa tgtcaaaatt aatgaacccg acaaaccaca gcgttggcga
     6841 agatggtgca attttattta atgcatggga tggaatagat attcgacaca cattcacaaa
     6901 aggtagcatt aaaactgatt ttgtagtcaa taatttaagt gcaattgacc caactagtgc
     6961 atatagtatt ttcaaagaaa ccattcaatt acctgatggc ttttctatta ttagagatat
     7021 ttacgaaggc gaagaatcta ttaatggacg ttgggttgga gatttaatat tagtggacgc
     7081 atttggtaat gaatacgccc gttatgacca tgtatttact tatatggaaa atccgattga
     7141 tgaggaaatg gctgcgttaa atttaattgg tgaatatgct attgaacaag ttggtaacct
     7201 tgttacatta tcgctttaca ttgataatag ttggttaact gatccggcac gaacatttcc
     7261 aattgttatt gacccaaccg taagtggaac actggctaca attgccggaa caccgcttgg
     7321 atcaaggttc tggcctgagt attgtgtcca tacaatgatg gtgtcaactc ccgcaaacgc
     7381 taccttaaca acagccaatg tagcgttaga tgtagacgcc aaagccggtg gttgcacgcc
     7441 aagtggtaat tgccagagaa attttacgca agtgtatatt tctacatcat gtggtgttga
     7501 cccaattggc gcacctggaa ttatttacac ctgtgttgga ggtggaactt gtttacttcc
     7561 cggtcattta gttttaccaa ctactagtat gccgcattta gtgttagatt gttttacgcc
     7621 cagttgtgca tcatttacaa ttccatttaa tgttcattta cagcggttag cctgaacaaa
     7681 tacagatcct ttatgtgatg tgccttgtat acaattagac ttatttgaag taactttaaa
     7741 aggtaaaaca gttgaagcta ccgcaacagc tgcaggagga acatcttata ccattttaga
     7801 ttgtactgat caatcagatt ggcttgctcc agatgaagat atttatggtg taccagcgta
     7861 tacttattca tggtcaccta taggcgctac tgacgatagt atttatgtaa cattcccatt
     7921 aggaacaact tcctacactt taactataac tgatgcttgt ggtaatattg caacagatgt
     7981 agttaccgta actaataatt gtattttact tccgctgcca ttaactgaat ttagcggata
     8041 tcatcaaaat gattttaatt tattgaattg gtctactcaa tcagaaattg gaacaaatta
     8101 tattattgaa cgctctgtaa cggggaatga ttttattcca attggcactg tcgaatcaaa
     8161 aagcaataca ggttttcaaa cctattcgtt ttcagattta agtccggaca accaaattgt
     8221 atattaccgt ttaaaacaaa ttaatacaaa aaacgaggtg ctgtattcag atgtaattgc
     8281 aattaaaacc gcgtataaac caggttatga aataatttca acaaattttg atgcagaaaa
     8341 taatatgctt caattaagta ttaattccga aaaagattta actgccgata tgataattta
     8401 tgatcttgcc ggcaaaattg tttttacaaa ttcaatagca ttagcgacag gcactacagt
     8461 agttaaatca acattaccgg agcttgcaaa aggagcgtat gtagtacata ttgtagttga
     8521 agatcaaaat ttagaatcca aatttgttaa ttgaaccttc aaatttcaaa ccttctagtg
     8581 agggtttagc tggtaaaaat tgcgttggtt atacctccgg actaatcctg tgattgccta
     8641 atttttgaga attccaccgc tttttcaaag catctgctta cctttgcgcc atgatttcag
     8701 tgcagaattt atctattcac tttggtggca tatttttatt tgaagatgtt tcttttgccc
     8761 taaaagaaaa cgatcgcgtg ggtttagtcg gcaaaaacgg tgcaggaaag tccaccctca
     8821 tgaaaataat gagcggttta caaaagccgg attcgggcca ggtaatgctt ccaaaggaca
     8881 gtctaattgg gtttttgcac caggatttga gtaaaatgaa gggcaaaacc gtgtttgaag
     8941 aaacacaaac cgcctttaat gaagcgttgc agttagataa acgcatgcac gaaattgaac
     9001 atgaacttac cgtacgcacc gactacgaaa gtgattcgta tcatgatatc attaacgaga
     9061 ttcaggaaat ttctcaccgt ttagagatta ttaacgtagc caccatcgac aaacaaattg
     9121 aattggtatt gatgggttta ggctttatgc gagaagattt cgacaaaccg gttgaaacgt
     9181 ttagcggtgg atggcagatg cgtattgaac tggctaagat attgttgagc aaacctgaat
     9241 tggtattatt agatgagcct accaaccact tggatattga aagtattcaa tggttagaaa
     9301 cttatttgga aacatatccc ggagcaatta tgattgtttc ccacgataga acatttttag
     9361 ataatgttac caaacgtact attgaaattg taagtcagaa aatttacgat taccctgcta
     9421 attattccac gtttgttgaa ttgcgtaaag agcgaattga tcagcaacgt gcagcgcaaa
     9481 aaaatcagga aaaatatatt gagcataccg aaaaactgat tgataaattc agagcaaaag
     9541 ccaataaggc aaaatttgca caatcgctca ttaaaaagtt ggataaaata gaagaaatag
     9601 aagtggacga tgaagaaact gcaactattc acttttattt tccaaaacca caacgttcag
     9661 gtaaaactgt tgttaccggt aaaaaagtct ctaaaactta tgatgtaaaa ttgattttag
     9721 atgaaattga ttttgatatt cagcgtggag ataaaattgc gtttgttggt aaaaacggcg
     9781 aaggaaaatc taccttgtcg aaaattattg ccggcattga aaaatatcaa ggtgaattag
     9841 aattgggaca caatgtgcat ttaggctatt acgctcaaaa tcaggctgaa acattgagtg
     9901 gcgacgatac ggtatttgat actattgata aagtggctac cggtgaaatg cgttcacata
     9961 tcagaacttt attaggagca tttttatttg gcggagaagc atcgtttaaa aaagtaaagg
    10021 tgctatcggg tggtgagaaa tcgcgactgg cgatgtgtaa attattgctt gagccatata
    10081 atctattggt actcgatgag cctaccaacc acttggacat gcgcagtaaa gatgtattaa
    10141 aaaatgcatt aaataatttt gaaggaacag taattgtagt atcccacgat cgtgattttt
    10201 tgaaaggaat taccaaccgt gtatttgaat ttaaggataa aaaaatcaaa gaacatattg
    10261 gtgatatata tgattatctg gaagctaaaa aaatatcttc actggcagaa ttagaattag
    10321 ctccgaaacc ggttaaacaa aaaaaagaaa ccacaacaat ttcgcctact aaagaaaacc
    10381 acgaagacaa aaaaaatcgt gagcgggaag taaatcgcat caaaaaagaa attgaaaaaa
    10441 ccgaaaagga aattgcggca atagaaacgg aaattgttca gcttgaaaaa caaatgaatg
    10501 atcctgggtt ttatgaaaag ccgaatacca ccgaaatttt caataaatac aacaacctga
    10561 aagaaagcca tacccaaaaa atgagtgggt gggaaagtct gacagaacag ctcgaaacaa
    10621 tttaatgaaa aataaatata atcaaacatt acgtagttcg tttcctttca tcgtatattt
    10681 gcgatataca attgaatatg gcttactcga aaaaacatga atttgacgct gaaagcatcc
    10741 gaattgctga tatagcaaag gctttgagcc atccggcgcg tattgaaata ctgaaagtgc
    10801 tctcgaacgg gcaatgtgtt tgtggggata ttgttgacca acttccgctt tcacagagta
    10861 ctgtttcaca acatttaaaa gaattgaagg ctgccggact aataaaaggt gaaattgacg
    10921 gaccacgagt ttgttattgt ttaaacgaag gggtttgcaa tcaggcactg ggatttttca
    10981 gacaattgtt tcgtgaaatt aaatgctgtt aagatgtata cgttccgaac tgcaactatt
    11041 gatgatttga ctgcaataca aacgctgttg atacaacatc aactcccagt tgatggatta
    11101 actcaacata ttcaacactt tgaagttgta atggcaaata atcacctaat tggagttgca
    11161 ggtgtagaac aatattttcc gtttgcctta attcgttcag ttgctattga tgcacaatat
    11221 cagcatcagc atattgggaa aaaattaatt gagaacttat tatcaaaatt aaataaagct
    11281 ggattcacta cttattatct gctgacagaa acagctccca catttttttc gaaattaaat
    11341 tttcacatta ttcaacgcag cgaggttcca cttgttattc agcaaaccca agaatttgca
    11401 acaatttgtc ccgcgagcgc aatagttatg acaaaaaata atttatcact ataaaatata
    11461 tattatgcaa aacgatcaag aagtaaaaga ccttgtaaaa gaacgataca gtgaaattgc
    11521 actgcaatca aaaaccacca acgcatcatc atgttgtggc gcaggtggat gttcaaccat
    11581 tgaccttgaa attatgagtg aaaattacac ttcactggaa ggttattttg ccgatgccga
    11641 cttaggttta ggctgtggta taccaactga atttgcacaa attaaaaagg gcgacacggt
    11701 ggtggacctg ggctcaggtg caggtaacga tgcctttgta gcacgcagca tagtgggcga
    11761 agacggccgc gtaattggtg tggacatgac agaaacaatg attgaattgt cgcgcaaaaa
    11821 tgcagcaaaa ctcaacttac aaaatgtaga gtttcgcttg ggcgacattg aaaatattcc
    11881 aattacttca aataaagcag atgtagtagt gagtaattgc gtattaaact tagtgccaaa
    11941 taaagtaaaa gctttttcag aaatgtatcg cattataaaa cccggtggtc actttagtgt
    12001 ttctgatatt gtattagttg gcgaattacc agcttccata ttaaaagaag cagaaatgta
    12061 tgccggttgt gtaagtggtg ctattcaaaa agatgaatat ttaaatatta ttgcacaaaa
    12121 tggttttaaa caggtaagta ttaaaaagga aaaagaaata attcttcctg atgatatttt
    12181 atccaattat ttaaatgaag aacaaatgac tgcatttaaa aatagtggtg taggtatatt
    12241 tagtattacc gtttttggtg aaaaaccaaa agactgtggt tgtggtacag attgctgtag
    12301 ctaataattt tttttatatt tttataacgc catttaaaat atttaaatgg cgttttttat
    12361 ttcccaataa atatgtttgc aaaacggctt tattgcaacg atatttgtac aaaaataacc
    12421 attgaataca ggggactgga tacaggtttt agggttaatt gttgtattta tgattttggt
    12481 aatcattatt tacagcatta taaactggcc atggtatgct tcaactgcat taattattgc
    12541 tgcattaatt gtcgggtggc tggttaaccg atttattaaa ttgtagggtt ataacttatc
    12601 cggtataaca gcagaagccc tttttgacaa gctttataac acccaattgt atttttcagg
    12661 cgagttcttg ctacctttgt agggtcaaat aaaccaaatt atgcaaaaat caattgtttt
    12721 aacattaagt attgccatgc tatcgctttt tggagcatgc caaaattcat cgcaggatgc
    12781 tatcaaagaa aagaggaaag aggaaatgac aaataaagaa aatcgtgtaa gtcctcccgc
    12841 aactgcaacc gcaacaatag gaacaaatac tgtaaccgta aactatagtt cacctgctgt
    12901 aaaaggccgc cctatttggg gagccttggt gccttatgga gaagtatgga gaaccggcgc
    12961 aaatgaggct actaccgtaa cctttacgca ggatgtcacc attcagggac aaaccctcac
    13021 aaaagatacc tactcttatt ttactattcc tacagatagt gtatggaccg ttatttttaa
    13081 cgtaaatgaa acacaatggg gtgcattcaa atacgatcaa agtgaagatg cattaagagt
    13141 gcaggttaaa ccggtaatgg tagaaactaa taccgaaaat ttatctttta gtgttgtccc
    13201 ggatgcaaca cctaattccg gaatcattcg attaaattgg gaaaaattgc aattagattg
    13261 tccgttcacc aacgttgcca actaattaat tataatatca ttctaacccg gttaattatt
    13321 ttaaccgggt tttttattgc taaataaatt cggaaattca ataataagtc aacataacaa
    13381 atgatgttgt atttgcgggt gttgttacag agtcgatgag tatacctaaa acaggatgcg
    13441 tagcttttat gtaataataa tctttgtcta aaccattaat ttctgcatgg ccattttcat
    13501 ctgtagttcg tgttgcttca ggataataat cgtaggcaag ttcttcgtaa ctcaaataaa
    13561 tatcaatttt aacatcctga acagtactat ccattaatgg ggaaatatca taaatccgat
    13621 tgacgtcaaa aataatggtt ctgttaaagt tctgactggc attaaaatta tcgtcaggta
    13681 actccgtttc attacatgca taaaaaccaa atgcaatgaa atacaatagg attacttgca
    13741 aataacatct cgccaacaac ttcatgtaat aaaattaccg ttaattcact gaattagata
    13801 atacgaatgt aatactaacg taatttttta agccaagtaa tttttattga ttaatagcag
    13861 caaacaatta ttctgttgtt tttacaaaaa tataaccgcc agtttctgct ccccaaacct
    13921 gcttcccatt ggcatcccaa ccacgatccc agcttaataa ttgatcttcg gtaataacaa
    13981 cttcgctggt agcataactg gcgccacgca aactactcgg gcaggatgta gcacctgtgg
    14041 aaccaacaaa tcgattgata ttgatatctc caactaactc atgttcgtat tttaaagtaa
    14101 tagaacaacc atcgcgtaaa atcaatttat caaatgtgag gttactcaat tttgactcat
    14161 cgttccatcc gccagcatat tgtaacggat tttcaaattc ataaacacga ctttcataaa
    14221 tacccaatgt aatttctact acctgatata ctctttggcg atatggttta tcttgcgcgc
    14281 tgcccaatgc ttgttctaca tacaaccaat tgccgggttc attacccatt tgaatttcaa
    14341 ccatgtgcaa acggatatca taaaaattag aatcagcctt gctttgtgct tcacttgaaa
    14401 atgaacctgt catatatgct aataaggttc cataaatatt ttcaagctgt gcttgcgact
    14461 gaaacgaaac taaaaaaaga aaaataaaaa cgagtttctt catttaatca accaattgtt
    14521 ttaatatggt attcatatcc acttttgtat ttacaatttg taatacatcg gcaatagcaa
    14581 tacgttcttg ttgcatgctg tcgcgttcgc ggatggtaac ggtattatcc tctaatgttt
    14641 gatggtcaac cgtaatacaa taaggtgtgc caatggcatc catacgacgg taacgttttc
    14701 cgatactgtc cttttcttcg taataacact ggtgattaaa tttcagcgta ttaaatattt
    14761 ccattgcttt ttccggcaaa ccatcttttt taattaatgg taatactgcc actttaattg
    14821 gagcaagaat agcaggtaaa cgcaatacca cgcgtgtttc ttcgccaaca ccttcttcgt
    14881 cataagcatt gcacatcata gctaaaaaca tacggtcgca accgatggat gtttctacta
    14941 cgtaaggaat ataattttca tttaattcag gatcaaaata ttgtaatttt tttccgctga
    15001 attctgtatg tcggcttaag tcgtgatttg tgcgtgaatg tataccttcc agttctttaa
    15061 atccaaacgg aaatttaaat tcaatatcca cagcagcgtt agcataaaaa gcaactttat
    15121 catggtcgtg aaattgtaac atactcgcat cagtacctaa agcgcggtgc cattgcatgc
    15181 gtgttttttt ccagtaattg taataattca tttcttcgcc aggacgaaca aaaaattgca
    15241 tttccatttg ttcgaattcg cgcatacgga aaataaactg acgtgcaaca atttcatttc
    15301 taaatgcttt accaatttgt gcaataccga aaggaatttt ctgacgtgta gttttttgca
    15361 cgttcaaaaa attaacaaaa ataccctgcg cagtttcagg acgcagataa acaacatcag
    15421 acgaatcgga cgcagcacca aaactggttg aaaacatcaa attaaactga cgcacatcgg
    15481 tccagttgcg tgtaccggaa atcgggtcaa caatttcgca atcaataatt atttgtttga
    15541 gctcgtcgag attgttatca tttaacgctt taacaaaacg tgtatgtaca gcttctcttt
    15601 tggcagcaat ttcaagcaca cgaccattgg tagttctgaa catcgcttca tcgaaatttt
    15661 cgaaacgttt ttttgctttt tctacctctt tatcaatctt ttcatcctgt ttcgccaaaa
    15721 attcttcaat cagcacatcg gcgcggtaac gttttttgga atctttgttg tcaatcagcg
    15781 ggtcgctaaa tgcatccacg tgaccacttg ccttccaggt agtagggtgc atcaaaatag
    15841 ccgaatcgag gccaacaatg ttttcatgca tctgcaccat agccttccac cagtattctt
    15901 tgatattatt tttcagcaaa gccccgtacg gaccgtaatc gtaaacagca ctcaatccat
    15961 cataaatctc tgaactctgg aatatataac catactcctt ggcatggctt atcacttttt
    16021 taaacttttc tgcgtcgttg gcaatcatgg cgcaaaggta aggggtttaa aggaagattg
    16081 gaaacaggca aagtgccttg gtttggcggc gaaaatgctg ttctcgaaac gtattttgat
    16141 gcattttagc ttaatgcgtt gaagctatgg taaatgaaaa gattgaaaaa aattaggaat
    16201 aaaggtattg acattacatc ttattgctta ctgtatttca tataaaaaaa aatttaattt
    16261 ccaattattt aattataaat ggatattcat tgcaattaag gttttccacg aatttaacga
    16321 ctttatattt gagtgatgct ccaatgacca atagaaaatt tgaccagtta agatgaactt
    16381 ctatatatgg taagtattcg ctaggcgttc aatttgcgtc cacagataag gtatattacc
    16441 tggatatctt taactgtgtt tcaggcaagg gatttgtagc gactaagcaa gataaattag
    16501 aaattatcga tggaatgctc gctacttgat acttttttga aaacgaaact tacacctata
    16561 cactgtatac attcactaca accgataatg tagtttttta taacgtgcaa gtgttcaata
    16621 atttcgggaa ataattaaaa agaaaggagg cgctatacat acaacgcctc ctttcttaat
    16681 ataccggttt gtttattgaa ccagtataat ttgttttgaa taactatatg aatttttatt
    16741 aatggctaca atgtatgttc catttcctac aaccgaatta aacttgttat ttaattcatt
    16801 taagtaaccc gaaatgacta actttccttc caagtttact acagaataat tagctcccaa
    16861 taattgttta tcaatattta tacaaatagt tccggaatag ctataaattg ttggtgcaaa
    16921 caaatttaat tctgatatcc cgacagtggt ggtatcacca attgtgtctc ctgccataat
    16981 tccagtttca catttggttt cgtaaccgta atcatgtata gttaaactat cagcttcgga
    17041 tgttatacta caacgaatcc aaccgtagtg gaaacaatct aaactatctt tgaaccggac
    17101 acctaagaat ttattctcaa gaccataaaa ccattgacca cctaaagctt gacttgcgcc
    17161 atcgtcacta ttaataatat atgccaaaat ttcaaagtta ttattttgaa atgttaggtc
    17221 ctcattaatt aaatgcattg agtttaaagc aaaaggaaag tattgagtaa atccgccata
    17281 tgaaggagaa atcacacttt ttaaccctgc cattttatta ccgctttgcc atggtgaaat
    17341 agcaatataa tatgcataag agatattttc actccaatac gaataaaatg agccacttat
    17401 tttttgaagt ttaaaatcta aaagcccatc gttgttcata tctaaattat atgcgtcgcc
    17461 atcttctgaa agaataatat ccggatcaat ttcagtataa attgcttcag cttggacgtt
    17521 tttacatagt aagtaaacta tagcaaaagc ggaatagttt aaaataaagt aattttgatt
    17581 ctttttcact gctttatgat tttttcttta aaaaacctcc cattgcccat ttcaacaaat
    17641 ataaggtaaa ttccactact taaataagat atatctattg ggctttccat gctgcaccaa
    17701 ttcttgtaac aaattttgcc atctatggtg gttattttca cctctaagcc actagtagtt
    17761 gcatcttcta ggccactaat cgagaaagaa atactttgat ttgatgggtt agggaaaact
    17821 aagaaatgtg gttcttttct ctcaattttt tcataaggag gataatcagc tattattggg
    17881 actttaaaat agccgaaaga atatgctgga acttcgacag tagtaaaagg gctcccactt
    17941 gacggagaat tagttagtgt gaaaaatgta tcaatttcaa tgttataatc tacgttataa
    18001 ttaatatcat tataacattt gtttattgta tataaagtat ttttacctcg gcccccagtt
    18061 gagtagggct gtagtgcatt gacacaataa atagttaccg tgtcggtaat ttgaacatgt
    18121 ccatctggtg gaaaaatacc tgatgttcct gtcaaattta tgtagtaagg ttgtgggtca
    18181 gcacttacat tgctatagta gaaataaaaa ttttcttttt ccgaatcaat gaagacggtt
    18241 ggctgaacgt ttatattatt tttagctaat gtaaaattag tttgtaaata ttttaacccg
    18301 ttttttggta tatcacttaa catcagcatt gtaaaatatg aagcacgttt catatagtaa
    18361 tttctgcctt ctaatgaatc tgtagaaata ttataaggga ttgtgtctcg attcaatgaa
    18421 tctagctctg gtccgaaagc cggacttaac atagcgctat gtgaagctcc ggcaaaatta
    18481 tgtaaagtgg catatttaaa aaaaccttct ctataagaat tattataatt aatttttaag
    18541 ttctttaacc accactcttg tagcatagct gaagttaaaa atgtttgtgc aaaaacaccc
    18601 agtttatctc tttcatatat agtataatca ccgccagttt ctttaaaatt atattctgat
    18661 gtaataattt ctttgccacc actagatact gacaaattta gaccaagagt atcccgatgc
    18721 acattccatg actcctgata tcgtgtatta gtaaattttt taaaattttt tgcaagccca
    18781 tcaaacgcat cttcaagtct tgggtcatgt atatcataac gccatcctat tgtatcaggg
    18841 ctttcacatt cataaacaga gtctaaatta taaagagcta ttgtatccca attgtgggta
    18901 tcataatatg gatgaactat atatgcatca aatgattttc taaaatggcc tggcaggcct
    18961 tcaatttcaa ccttttctaa tcgtttttca aacaggtcgt cattccattc atttgctgct
    19021 cgaatacctg aagaggcggc ctctcgaaat gcataagtgg gatcctttaa gttttccact
    19081 ggtagtccaa ttttgcatgt aaacagagga gagcttttaa atgttgaaat ataatcatgg
    19141 tcaaaccaaa catcagtgcc aatcaaaaca ctatcaagac tatcagtaat tcctccattg
    19201 atataggtcc aatagtcttc cagagtataa attcctagca tggtctcaga aaatgaaaaa
    19261 tacatttcat tgccaagctc aacatacaca acgtttacat tgcaagtgtc gtgattgcgc
    19321 aaatattcga caatttcctt gctccttgta gccgtttcat tcattatatt tagatcaatt
    19381 attacatcaa ccttttgacc tggatttgca acttctatct gtttaaccat atacacaaaa
    19441 tcgtctatgt agagatgatt agaatctaat aaattttgtt caacccactt atctgcaaac
    19501 ccaatgaagt catctacctc cttagctgta atccaagtaa gataaaagag tgctcctgcg
    19561 tcagcctcta tgctatcttt tattgatata taggaaggcg catttaccac tgagtcagtt
    19621 ctatcataaa aacggattat ctcttcaatg tcatagccat acccaggacc atctagtaag
    19681 tgcataaatt tactagatga acctccagga aacctgagac ttgacggagc caattcactc
    19741 atccattgcc attggtcaac ggcagaacca tcatttggaa tcacttttct actataaatc
    19801 ccctcaatgt gaaacccata taggccttta tttacagact tttgagtgct gcctaagttt
    19861 atataaattg tagagtctgc atggactttt gaatattgcg agatattgtt agaattttga
    19921 taatatgtag ttccttctgt ttttaaattc attccctcag ttggtccatc tataccccaa
    19981 taccaaacgg tgtcgagtcc atctgttaag gtttcttgaa ttaagcctga tgtatcgaac
    20041 gtttcctcat caggaaaata ctgagcaaaa agtttgttag aagtgagtaa aataattaaa
    20101 aaaagcgaaa tagcggttgc cattaatttg ttatttgagt ttttcattag agtaaattgg
    20161 gttttaagaa agttaaaaaa acaatacttt tctaggtgaa tgttaaacaa atctaacgaa
    20221 taacttaatg aattttgtta aattacattt tgttcaataa attcatggaa aaccgttaat
    20281 caaattcagc attcgttaat tagagctgaa aattattatc aattacataa gtattctaaa
    20341 atataaatgg tataagcaac cggtgaagag tcattattca catatgttta tctctttaaa
    20401 ttttaattaa accctgattt ttataatttt actatcaatc taaaattatt tattcaaaat
    20461 gt
//
LOCUS       DAORKW010000012        19959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_20_length_19959_cov_6.219855, whole genome shotgun sequence.
ACCESSION   DAORKW010000012 DAORKW010000000
VERSION     DAORKW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19959
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20_length_19959_cov_6.219855"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLB46_01145"
     CDS             complement(<1..861)
                     /locus_tag="PLB46_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRF75146.1"
                     /translation="MIAQADGQILISFQNSILQGIPAELPEPKPYDTSISHAPKRKDI
                     LNADEKRLAIRNALRYFPTKFHATLAPEFAEELQKYGRIYMYRFRPDYTIKARSWEQY
                     PHKSKQAACIMLMLQNNLDYAVAQHPHELITYGGNGAVFQNWAQYLITMQYLANMTDE
                     QTLVLYSGHPMGLFPSHKRAPRVVVTNGMMIPNYSKPDDWEKFNALGVTQYGQMTAGS
                     FMYIGPQGIVHGTNITVLNAARKIDKQAIDCSGKIFVTAGLGGMSGAQPKAAVIAKGV
                     CVVAEVNPEAT"
     gene            949..1377
                     /locus_tag="PLB46_01150"
     CDS             949..1377
                     /locus_tag="PLB46_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75147.1"
                     /translation="MKKFILGLCLTPFLFTACEKDVVPDYDIRDEYVGDYQVNDACDA
                     SMNDYTISVFKANDNDEIVFGWPGLYEAGMEVHGIVTGMKVIIPIQQFLVSSFPEIYY
                     EFSGSAALEGDTLIVDYNVLLIQDGLIFDENNCRATMVQF"
     gene            complement(1374..2600)
                     /locus_tag="PLB46_01155"
     CDS             complement(1374..2600)
                     /locus_tag="PLB46_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75148.1"
                     /translation="MKRFLFLTFVYGIVTLNAQSPFTIYNEDNSDLPVNQVNKIVVDD
                     LNNKWVGTENGLIKIDASGVWTLIDTANSDIPANEIRSIYVIDEEHYFVGTYLNGLGY
                     YNAGVWTNYTPDNSPLADYHIKAINMTSADTIWIGTPSGLIKWDGADYWFVYNTGNSL
                     LHSPNIIDVYIDENDDVFIGTLNGGLSTYIDGFIDYYRTDNSLISDNTVMSIAEDDLH
                     NKWLATSFGGLSVFTPDETFLKFTPLTSDISDWSVDAVAIDDNNIGLLAMASTGLNIF
                     DNTDWTILTTENSDLPDDFVNTVVADNDQLVWLGTDTGGLVVLNRDLLNDIEQTTTIT
                     MQVYPNPATDNCIFSGNFNEGEIRIYTLSGVLVYTQKIKSNTNFIQVSDFISGNYIIQ
                     FTNDEGAATLPLSIIH"
     gene            2717..3733
                     /locus_tag="PLB46_01160"
     CDS             2717..3733
                     /locus_tag="PLB46_01160"
                     /inference="COORDINATES: protein motif:HMM:NF025464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HRF75149.1"
                     /translation="MRPFNPFPLAVLCVFILSSSCKDNTAGCPAVADVITSFVSESGD
                     IALVTNSGEAYLQSDDGCTFLEQYFTPGFEDIYVFTDDELFVTTDDGSLFPVTNTFYD
                     EVNDYAVLQDAIILSTDDSTLHWNSFVLQSPLAPSVEDYVALRACLLNNTCDFLDNRI
                     DIVSDPENITNKVFQFTAVAPSANMVTSKSSIERTFNYFVKGMNLWYEAKYYIADGMP
                     YSIADFENSYFNESPGPRIVFNGNALAIENKFGDKIKYYQNTPVVFPTGEWVTVKVHF
                     MFSETNNGYIELWQNGIRIMEVTGINLPLFNSIQNSLEVGISATQTGCVLLVDDVRLS
                     PIPF"
     gene            3800..4159
                     /locus_tag="PLB46_01165"
     CDS             3800..4159
                     /locus_tag="PLB46_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75150.1"
                     /translation="MKKIVLATVFTFGAFYLQAQNHDLPPAEQRAKKQTELMDQKLNL
                     DDAQESAVADINLKFAKRVDEVFAADESKKEKAKKMKKINDEKEAALQPVLTPEQFAK
                     YVELKDEFVEKIKERHQ"
     gene            complement(4225..7635)
                     /locus_tag="PLB46_01170"
     CDS             complement(4225..7635)
                     /locus_tag="PLB46_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF75151.1"
                     /translation="MKDVFKEFKPSSWAIDNKTTIFIITIMLTIMGISTYNALPKELY
                     PDITMPTVYVATIYPGSSPADMENLITKELEKEIGAISGINKIISNSVQDFSSIQVEF
                     VSGTDIDDANQKVKDAVDKAKPNLPSDLDPTFGPNVQKVEFSDFPILQVNVSGDYDLA
                     TIKKYAEKIQDEIEELPEVSKVDIIGAPEREIQINVDMYKMQAAMLTMYDIYAAVSNE
                     NRIVSGGTIKVEDMRRSITVSGEFDNVAQIQNLTVNNANNYPIYLKDIAEIKDDFKEQ
                     ESFARLDGKNVITLNVIKKSGENLIEASDNINALLVELEKKEIPSDVDVTVTGDTSTL
                     TRTQVADLINTIIIGFILVTIVLMFFMGATDAMFVGLSVPLSMFIAFIVLSLLGISMN
                     LIVLFAFLLGLGIVVDDAIVVIENTHRIFANGKVPIKTAAKNAAGEVFLPVLAGTMTT
                     LAPFFPLLFWPGMIGSFMKGLPITLIITLTASLLVAYLINPVFAATFMKPHHDTDKPN
                     RRKQGIWSAIIGGLGLLISLGGNPLLGNVMITFVAIFWFYNLVLIKAVKGFQKRGWPN
                     IQARYASILRWTLKHPIILFSSIIGTLILAIVMLGITKPGVEQFPTGDPNFIYTYVVM
                     PDGTDQQTTDSVTKLVEKRIYNVLGENNPLVKSVISNVTIGANEDPFDQSSYTNKGKV
                     GVAFIDPADRRHQMSEPYLDKFREAVKDIPGAEITVGAEQNGPPAGKPINIEISGDDF
                     DELIKTSNDIKRYLESLNIAGIEELKTDLQANKPEITIDLDRERMNREGISTLTVGGE
                     IRNAVYGWEVSKFKEGNDDFPIVIRYDETQRKNIDQLKDLKITYRDMMTGQIRQIPLS
                     AFAEIKYSTTYSGVKRIDQKRVVTLASNLLSGYESAQPAIMNEIKAALEDYDTPEGVS
                     IGYTGASQDMEETISFMGVAWIISLFLIVIILVAQFNSISKPIIIFAEVIFSIIGVLL
                     GYGIFGMDFSAVMTGVGVVALLGIVVRNGILLVEFTDLLIAGGMPIREAVVEAARIRM
                     TPVVLTATATILGMIPLAIGFNIDFVGLFTQGRPDIWLGGENVVFWGPLAWTIVFGLG
                     YATFITLLVVPVMYLYNDKFKTWVFNLLGIKREVHHHGATIPGMLDKE"
     gene            complement(7694..8809)
                     /locus_tag="PLB46_01175"
     CDS             complement(7694..8809)
                     /locus_tag="PLB46_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF75152.1"
                     /translation="MRKTLLYLTVIVFLASCGAKESGDKQAELESLKKERSALDDKIK
                     AIEKELALNDTTKSDKGELVVVTEIQPIPFTHYIELQGKIDADENVIVTPQIPGVIDA
                     VYVVVGQSVSKGQVLAETDNDAMVKNIETLQSQLAFAKDVFNKQKALWDQKIGSEIQY
                     LSAKNNVETLEKSIAAANEQISATKLISPISGVVDAVDVKVGQIGSPGFQGIRVVNMR
                     TLKAKGEVSESHAEFVSAGDNTKIILTDLNKEIDAKISFASSVINPQSRTFSVEAKLP
                     SESNLKPNMIAVIKIADYEVANAMVVDLNMIQKSTDASFVYVAAEENGKSIAQRRVVT
                     VGKIYNGMAEITSGLEKGDKVITSGYQNLIAGQEIKY"
     gene            complement(8822..10138)
                     /locus_tag="PLB46_01180"
     CDS             complement(8822..10138)
                     /locus_tag="PLB46_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF75153.1"
                     /translation="MAYKLKAFLIALVVPFALFAQSQQYSLQEAMDYGIKNNAQILSA
                     ALDEQDAQITVNNRIASGLPQIDASLDYQNFFKLPTSLIPAEFFGGEPGQFIPVQFGT
                     EQNMTAQISASQLLFNGAWLVGISAAKEYAALVQKQTRLAETEVKKNVETAYYSVLIA
                     KEFEKVLQKNIENLDAVLFEVTEMQKQGFVEGIDVDRLTISKLTLNGQLENAQRQTQL
                     ATNYLKFSMGLDMATNIELTDSIGAMTEVNMVGTNYLSRPEFSILQSTITLNELNVKM
                     NKALFLPTLVAYGSYSQNAQRNSFDFLDGDQEWFETGLFGLTLSIPVFSGMERKTGLQ
                     AAQIDLDQARIQQQLATQGILLEIEKAKTDYINAENDLNVQTQNIALAQRIYDTALIK
                     YREGVGSSLELNSAESTLYQAQGNYVSAMFNMLNAKTALNKSLGYY"
     gene            complement(10128..10742)
                     /locus_tag="PLB46_01185"
     CDS             complement(10128..10742)
                     /locus_tag="PLB46_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF75154.1"
                     /translation="MSEEAKILEKCERLFMRYGIKSVTMDDVSRELGMSKKTLYQYFE
                     NKEELIHKVTQNHFTCQNKVVEHIIHHSKTAIDEMIGISNWMNTMSKNLNPSLVFDLK
                     KYHPQSWQIFNDHRNTEVYNCIKHNLERGIKEGLYREELDTEVLARIYIARMEMFLDA
                     EIFPYDKFPPEKTFNVFMDYHIRGLATTKGIKYLEKIKSENNGI"
     gene            complement(10993..12168)
                     /locus_tag="PLB46_01190"
     CDS             complement(10993..12168)
                     /locus_tag="PLB46_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF75155.1"
                     /translation="MRKIAADIIFPIHTQPLTNHVLVLEDDGTVADILPITELDNASI
                     EKVSGALVPGFVNAHCHLELSHLKNKFPEKTGLPQFLSLVTQQRNVPIEIINEAMELA
                     ENEMITNGIVAVGDISNNSNSFYIKAKRNLYYHTFIELIAFDPARANGAMQNGEQLLS
                     AARGYGIHASLAPHATYSVSPDLIKSINASCYANGKPTSIHMLESNDENEFYIQASGL
                     YRKLYRELGVDIKHFNPTGKTALESLLPYFNKEVSTLLVHNTIATSWDAEWAEDIHPN
                     LFWCFCPKANLFIEDRLPDIPMLMQHVQYIAVGTDSLASNHGLSVLSEIQTIQNKFPE
                     LPTSDLLRWATQYGAKALGINNQYGTFEKGKRPGVNVLSGITSTQTKLADTLVSRLV"
     gene            complement(12214..12870)
                     /locus_tag="PLB46_01195"
     CDS             complement(12214..12870)
                     /locus_tag="PLB46_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75156.1"
                     /translation="MKVKLFVLSMSLISCVTIFGQGQQAGTLSFQAGYDFGIHGTLYK
                     SQYLGLPLETDTGGAATSMFSFGAQYNLLNWLSGGATFVYGSYLEDTSDVSANGNNVG
                     TIAFDLRLYPVNGEKFNLYIGPEVGYSFLEIRKLNPSLGNIEEVSNYSGGHFGVNLGF
                     NWYIFSFAGVFAQAEYTRNDFTLKQYYIDGETFDLTNWDASLNTFSGAVRVGLSFKIN
                     "
     gene            complement(12966..14090)
                     /gene="mqnC"
                     /locus_tag="PLB46_01200"
     CDS             complement(12966..14090)
                     /gene="mqnC"
                     /locus_tag="PLB46_01200"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HRF75157.1"
                     /translation="MELGKLYQKALNLEFLSVEEGMYLFENAPTAELMQVADTIRRRK
                     KPGNKVTWIIDRNLNTTNICVANCKFCNFYRVPGHAEGYITDKETYRIKIEETLKYGG
                     EQLLLQGGHHPDLGLEFYAKTFREIKEMFPQIKLHSLGPPEIHHIASLGNHTYEHVLT
                     ELVKAGLDSLPGAGAEILVDRVRKIISTGKCTADEWLNVMRVAHKLNITTSATMMFGH
                     IETIEERFLHLEKIRKVQSEKPAHAKGFLAFIPWTFQDVDTTLKKIRRAKNDTTPDEY
                     IRTIAICRMFLPNIENIQASWLTVGKNVAQICLHAGANDFGSIMIEENVVSAAGAPHR
                     FTYKTIQDAIREAGFEPQLRNQQYEERAIPENIVEQEINY"
     gene            14223..14306
                     /locus_tag="PLB46_01205"
     tRNA            14223..14306
                     /locus_tag="PLB46_01205"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14255..14257,aa:Ile,seq:cat)
     gene            14410..14483
                     /locus_tag="PLB46_01210"
     tRNA            14410..14483
                     /locus_tag="PLB46_01210"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14444..14446,aa:Ile,seq:cat)
     gene            14515..14817
                     /locus_tag="PLB46_01215"
     CDS             14515..14817
                     /locus_tag="PLB46_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRF75158.1"
                     /translation="MPFYTYILFSKSLNKFYIGSTSLSPVERLEQHNQIKFPDAFTRK
                     GIPWEMFLVIECNSFIQAKAIELHIKKMKSTRYLQNLKQYPEIGENLLLKYRATDS"
     gene            15368..15796
                     /locus_tag="PLB46_01220"
     CDS             15368..15796
                     /locus_tag="PLB46_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75159.1"
                     /translation="MNAEQFEKIKQLSTKNNRIGVVAKLILEEISSVPYEEPLEEINL
                     ESDEDYKEQSEINETGAIQIYPHPVGSQSVIEILLSDLPENSRFIIYDLHGKVLFNYD
                     LLQQSTLIIVNNDMLTSGLYLGVVTSNNMIKQSIKFVVNE"
     gene            15789..17330
                     /locus_tag="PLB46_01225"
     CDS             15789..17330
                     /locus_tag="PLB46_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75160.1"
                     /translation="MNKYFRIVFLQISILFFLPEIKSQNTYNILIDHENQSEWGYSLL
                     MDMDTIVMTGYTNVLKDGFYRDGVFFRKLDLEGNIFRENLIADTCVLYTVGYPGNLVK
                     IDNQYLTSGIYQYCDYNFEGGVGSFGMVYKFNEIGDTIWTKKFDSQSYSIIYKGATTT
                     NNSYLSAGVSVDTATLIDKFYIIKIDSTGEIVWENTFGYLLNNYAFSIDSYTNGDFII
                     GGLTDPTTSFSQTDGYLAKLDSSGNLIWQKKIGTPDDDNGCIARITFDQQNIYLQQSL
                     DTVVSIGDAPMPDYVGKLDYNGNFIWRTFFNGPYKQDIWGIREMEKKSIMVVGTKIPD
                     DVSQGSGFICKLDSNGVKLWERTYYTRTDRDNYFTDVAKVSDGGYILTGSAWSATDRD
                     VWLVRLDSMGCLEPGCDIVDVPTITVNNTTLFNFYPNPMHNTATVELQIPYNFQILSG
                     ATIMLQIMDVSCKIVDTYANIPISNPGETIRFDVYRKNLAAGYYHALVKYNNVNFGSY
                     KFVIN"
     gene            complement(17436..18413)
                     /locus_tag="PLB46_01230"
     CDS             complement(17436..18413)
                     /locus_tag="PLB46_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF75161.1"
                     /translation="MKTISILIPETAVPAAVVDPRYMFSAVNEFHRAAGLPIPFKVQL
                     VAAKHEILLNQGSFSVRPDLLLTEVGETDLIIIPALSGDMETAIALNSDLIPWVVNRY
                     KSGSEVASLCVGAFLLASTGLLDGKSCSTHWMFANQFRQLFPEVTLADDRVIVDQNGI
                     YSSGGAMAYWNLLLYLVEKYTNKEMAIMASKFFLLNVNLNSQSPFSIFKGQKDHGDCV
                     ILEAQVYIETHYKNKITVDELAALSNLSRRTFERRFKKATSNSPAEYLQRVKIEAAKS
                     LLESGRKTVNEVMFEVGYADLKAFRDVFKKTAGMSPVDYRKKYESGLYG"
     gene            18509..18928
                     /locus_tag="PLB46_01235"
     CDS             18509..18928
                     /locus_tag="PLB46_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75162.1"
                     /translation="MENHTEITTIPNDIVVCCIRAESFPGGVLAAHQELHRLIPQKPG
                     RMYYGISYPDVTRKILYKAAVNMLNYDEPEKLNCETDVIKAGKYYSTILTDFMNNIPA
                     ISHIFNQLLQHPDLDPEGYCLEIYMNDKDVQLLVKLI"
     gene            18945..19361
                     /locus_tag="PLB46_01240"
     CDS             18945..19361
                     /locus_tag="PLB46_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF75163.1"
                     /translation="MATQIFINLPVKDLQKSIGFFTYLGFTFNPQFTDETATCMIISE
                     NIYVMLLIESRFQEFTKKPVSDAKSSTEVLICLDAESREAVDTMVKNAVDAGGRIYAD
                     PMDHGWMYGHSFEDLDGHQWEVAYMDITAFPQPENN"
     gene            19364..19774
                     /locus_tag="PLB46_01245"
     CDS             19364..19774
                     /locus_tag="PLB46_01245"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HRF75164.1"
                     /translation="MISNIIPYINFNGNCEAALTFYQSCLGGEIKLLPVKESPAAPQL
                     PSEMHEKIMHGEIKFGNMMIMATDINDQRSQTGNAVSLFIHFTEKDELMRAFEALSAG
                     GVITLAVANQSWGATYGELIDKFGIKWRCNFHQM"
BASE COUNT         6278 a         3764 c         3372 g         6545 t
ORIGIN      
        1 ggttgcttca gggttcactt ctgcaacaac acaaacacct tttgcaatta ccgctgcttt
       61 aggttgcgct ccgctcatgc ctcccaatcc tgcagtcaca aatatttttc ctgagcaatc
      121 tattgcttgt ttgtcaattt ttcgcgcagc atttaatacc gtaatattcg taccgtgcac
      181 aataccttgt ggcccgatat acataaatga acctgcagtc atttgtccat attgtgttac
      241 acctaatgca ttaaattttt cccaatcatc cggcttcgaa taattcggaa tcatcatacc
      301 atttgttaca actacgcgag gtgctctttt atgcgatgga aataatccca tcggatgacc
      361 actatataat accaatgttt gttcatcggt catattggcc aaatattgca tagtaattaa
      421 atattgcgcc caattttgaa atactgctcc atttccacca tacgtaatta attcatgagg
      481 atgttgcgct actgcatagt ctaaattatt ttgcaacatg agcataatac atgctgcctg
      541 tttactttta tgcggatact gctcccagct gcgtgctttt atcgtatagt caggacgaaa
      601 tcgatacatg tatatcctgc catatttttg cagttcttca gcaaattcag gggctaaggt
      661 tgcatgaaat tttgtgggga aataacgaag tgcattcctt atggctaatc gcttttcgtc
      721 agcattcaaa atatccttcc gcttaggggc atggctgatt gaggtgtcat aaggcttagg
      781 ctccggtagt tcggccggaa tgccttgcaa tatgctattt tgaaaactta tcagaatttg
      841 accatcagct tgagctatca tacactattt ttgcacaaag ttaccaattg atgccgcttc
      901 ttacaatgta ctggcacgat gttggcttaa cgatataaaa acatgcttat gaaaaagttt
      961 atacttggtc tatgcctcac tccattttta tttactgcct gcgaaaaaga cgtagtgcct
     1021 gattatgata tacgagacga atacgttggc gactatcagg taaacgacgc atgtgatgct
     1081 tcaatgaatg attataccat ctccgtgttt aaagcaaacg acaatgatga aattgtgttt
     1141 ggctggccgg gtttatacga agccggtatg gaagtacatg gtattgttac cggaatgaaa
     1201 gttattattc caattcagca gtttttggtt tcctcatttc ctgaaatata ttacgaattt
     1261 agtggaagtg ccgcattaga aggtgataca cttattgttg attataacgt gttattaatt
     1321 caagatggat taattttcga cgaaaataac tgcagggcaa ctatggttca attttaatgt
     1381 ataattgaaa gtggtaaggt ggcagcacct tcatcattag taaattgaat aatataatta
     1441 ccgcttataa aatccgatac ctgaataaaa ttggtattgg attttatttt ttgtgtatat
     1501 accaatacac cacttaacgt ataaattcga atttctcctt cattaaaatt acctgaaaaa
     1561 atacagttgt ctgttgctgg atttggatat acctgcatag taattgttgt agtttgttct
     1621 atatcattta ataaatcgcg gtttaatacc actaaaccac cggtatctgt tccaagccac
     1681 accaattggt cattgtcagc cacaactgtg ttaacaaaat catccggtaa atcagaattt
     1741 tcagttgtta aaattgtcca atcggtatta tcgaaaatat ttaatcctgt tgaagccata
     1801 gctaataatc caatattatt atcgtcaatt gcaactgcat caaccgacca gtctgaaata
     1861 tcagatgtta gtggagtgaa ttttaaaaat gtttcatctg gtgtaaatac acttaaccca
     1921 ccaaaagaag tcgccaacca tttgttatgt aaatcatctt ctgcaattga cattacagta
     1981 ttatcgctga taagtgaatt atccgttcta taataatcta taaatccatc aatataggtg
     2041 ctcaatccgc catttaatgt accgataaat acatcatcat tttcatcgat atacacgtca
     2101 ataatattcg gtgaatgtaa taatgaatta ccggtattat atacaaacca ataatctgca
     2161 ccatcccatt ttattaatcc gctaggtgta cctatccata tggtatcagc actagtcata
     2221 tttatcgctt taatatgata atctgctaac ggtgaattat cgggcgtata attagtccat
     2281 acacctgcat tataataacc taatccattg aggtaagtcc caacaaaata atgctcctca
     2341 tctatcacat atatgctacg aatttcatta gccggaatat ctgaattggc agtatctatt
     2401 aacgtccaaa cgccacttgc gtcaattttt attaacccat tttcagtgcc cacccattta
     2461 ttattcaaat cgtcaacaac tattttattt acctgattaa ccggtaaatc agaattatct
     2521 tcattataaa tagtaaaagg agattgtgca tttagtgtca ctattccata cacaaatgtc
     2581 aaaaacaaaa atcttttcat gtggcaaatt tacaattcct tagcagctct gttaaggcaa
     2641 gtttggtgat atttgtcaat gtacactcac taacaggagg tttattaacg ttatatttga
     2701 ttaaacacta atatttatgc gccctttcaa cccatttccc cttgccgttt tatgtgtttt
     2761 tatactgtca tcttcttgta aagataacac agcaggatgt ccggccgttg cagatgtcat
     2821 tacttccttt gtatctgaat ccggcgatat tgctttagtt actaattcgg gagaggcata
     2881 tttacaatcg gatgatggtt gcactttttt agaacaatat tttaccccag ggtttgaaga
     2941 tatttatgta tttactgatg acgaactttt tgtaacaacc gatgacggct ctctttttcc
     3001 ggttacaaat actttttatg atgaagtaaa tgattatgct gttttacaag atgccattat
     3061 tctttctacc gatgacagca ctttgcactg gaatagtttt gtgttacaaa gtccgcttgc
     3121 accaagtgta gaagattatg tagcgttaag ggcttgttta ttaaataata cctgtgattt
     3181 tttagataat aggattgata tcgtttccga cccggaaaat attacgaata aagtatttca
     3241 atttactgca gttgctccaa gtgcaaatat ggtaacatca aaaagtagta ttgagcgtac
     3301 atttaattat tttgtaaaag gtatgaactt atggtatgaa gcaaaatatt atatcgccga
     3361 tggaatgcct tacagtattg ctgattttga aaatagttat ttcaacgaaa gccccggacc
     3421 cagaattgta tttaatggca acgcgttggc gatagaaaat aaatttggcg ataaaataaa
     3481 atattaccag aacactccgg ttgtattccc aacgggcgaa tgggttacgg ttaaagtgca
     3541 ttttatgttt agcgaaacaa ataacggtta tattgaactt tggcaaaatg gtattagaat
     3601 aatggaagtt acaggcatta atctaccgct cttcaattcc atccaaaaca gcttggaagt
     3661 cgggatttca gccacgcaaa caggctgcgt tttgctggtt gatgatgtgc gtttgtcgcc
     3721 tattccattt taaaggcagg gtttttgtat ctgattaaac caaatgctta gttattagtc
     3781 aaaacaaaaa cctttatgta tgaaaaaaat agtattagcc actgtattta ccttcggcgc
     3841 cttttattta caggctcaaa atcatgattt gccacctgcc gaacaaagag caaaaaagca
     3901 aactgaatta atggaccaga aattaaatct tgacgacgcg caggaaagtg cagttgcaga
     3961 tattaattta aaatttgcca aacgtgttga cgaagttttt gctgctgatg aatcaaaaaa
     4021 agaaaaggca aaaaaaatga aaaaaataaa tgatgaaaaa gaagcagcat tgcaaccagt
     4081 gttaacaccg gaacaatttg caaaatatgt ggaattaaaa gatgagtttg tagaaaaaat
     4141 aaaagaacgg catcaataat taatcgctgt ttatgaaata aaaaaagcgc ctgaatatca
     4201 ggcgcttttt ttgtagcagt atgattattc tttatccaac atacccggaa ttgttgcgcc
     4261 gtgatggtgc acttcgcgtt ttattcccag taaattgaat acccaagttt taaatttatc
     4321 gttgtataaa tacataaccg gaacaactaa taaggtaata aatgttgcat atcctaaacc
     4381 aaaaacaatt gtccatgcta aaggtcccca gaacacaacg ttttcaccac ctaaccaaat
     4441 atcaggtctt ccttgtgtaa ataatccaac aaaatcgatg ttaaacccaa ttgccagcgg
     4501 aatcatacct aagatggtgg cagttgcagt taatacaacc ggtgtcatac gaatacgggc
     4561 tgcttcaaca acagcttctc taattggcat tccacctgca ataagcaagt cggtaaattc
     4621 aaccagcaaa ataccatttc gaacaacaat tcccaacaag gctacaaccc caacacctgt
     4681 cattaccgct gagaagtcca ttccgaaaat accataaccg agtaataccc cgatgatact
     4741 aaatataact tccgcaaaaa tgatgattgg tttagaaata gagttaaact gtgccaccaa
     4801 aataattaca atcaggaata aagaaataat ccaggcaacg cccataaagc tgattgtttc
     4861 ttccatgtcc tgactagcac ctgtgtaacc tatcgaaaca ccttcaggtg tgtcatagtc
     4921 ttccaaagcg gctttaattt cattcataat tgccggctgt gcactttcgt aaccggataa
     4981 taaattagac gctaaagtca ccacacgttt ctggtcgata cgtttaacac ctgaataggt
     5041 agttgaatat ttaatttcag cgaaagcact taaaggaatc tgacgaattt gacctgtcat
     5101 catatcgcga taagtaattt tcaaatcttt aagctggtcg atatttttcc tttgtgtttc
     5161 gtcataacga attacaatcg ggaaatcatc atttccttct ttaaatttag aaacctccca
     5221 gccgtatacc gcattcctta tttcaccacc aacagttaat gtagaaatac cttcacgatt
     5281 cattctctcc ctgtcgaggt caatagtaat ttcaggttta ttggcttgta aatcggtttt
     5341 taattcttca attcctgcaa tatttaaact ttccaaataa cgtttaatat cgttgctggt
     5401 tttaattaat tcatcaaaat catcacctga aatttcaata ttaatcggtt tacctgcagg
     5461 tggtccgttt tgttcagctc caacagttat ttcagcacct ggaatatctt ttaccgcttc
     5521 gcggaattta tcaagatatg gttctgacat ttgatgacga cgatcggcag ggtcgataaa
     5581 cgccacacca actttacctt tgttggtata agaactctga tcaaatgggt cttcattagc
     5641 tccgatagtt acgttgctga ttacagactt aaccaaagga ttattttctc ctaatacatt
     5701 gtaaattctt ttttcaacta atttggtaac tgagtctgtt gtttgttgat cagttccgtc
     5761 aggcataact acataagtat aaataaagtt agggtctccg gttggaaatt gttcaacgcc
     5821 cggtttagta atacctaaca ttacgatagc taatattaaa gttccaataa ttgaagagaa
     5881 taaaatgatt ggatgtttta atgtccatct gagtatcgat gcatatcttg cctgaatatt
     5941 tggccatcca cgtttttgga atccttttac tgctttaatt aatactaaat tatagaacca
     6001 gaaaatggcg acaaaagtaa tcatcacatt tcctaataat ggattaccac ctaatgaaat
     6061 taataaacca agaccaccaa ttattgcaga ccaaattcct tgttttcttc tatttggttt
     6121 atcagtgtcg tgatgcggtt tcataaatgt tgccgcaaat accggattaa ttaaatacgc
     6181 taccaacaac gatgcggtga gggtaataat taatgtaata ggcaaacctt tcataaatga
     6241 accgatcatt cccggccaga ataataaagg gaaaaacggt gctaaagttg tcattgttcc
     6301 tgcaagtacc ggaagaaaca cctcgcctgc agcattttta gcagctgttt taattggcac
     6361 ctttccattg gcaaaaatcc tgtgcgtatt ttcaattacc acaatcgcat catctaccac
     6421 aatacccaat ccaagtaaga aagcaaacaa cacaatcaag ttcatgctta tgcctaataa
     6481 acttaaaacg ataaaggcaa taaacattga aagtggtacc gacaaaccaa caaacatcgc
     6541 atcggttgca cccataaaaa acatcaatac aatggtaacc agtataaatc cgataataat
     6601 cgtattaatt aaatccgcaa cctgggttct ggttaaggta gaagtatcac ccgtaaccgt
     6661 aacgtcaaca tcacttggaa tttctttttt ctccaattca actaaaagtg cattaatatt
     6721 atcagatgct tcaattaagt tttcaccact ttttttgata acatttaaag tgattacatt
     6781 tttgccatct aaacgcgcaa aactttcctg ttctttaaaa tcatctttta tttcggcaat
     6841 atcttttaaa tatatcggat agttattggc attgttaacc gttaagtttt gaatttgtgc
     6901 aacattgtca aattcaccac ttaccgttat ggaacgacgc atgtcttcca ctttaatagt
     6961 accacctgat acaatgcggt tttcgttgct aacagcagca tagatatcgt acattgtaag
     7021 cattgctgcc tgcattttgt acatatctac attgatttga atttcccttt ccggtgcacc
     7081 aatgatatct actttagata cttccggtaa ttcttcaatt tcatcctgaa ttttttcagc
     7141 atatttttta atggtagcta aatcatagtc accactaacg tttacctgaa gaataggaaa
     7201 atcagaaaac tcgacttttt gtacattagg cccaaatgtt ggatctaagt cgctcggtaa
     7261 attaggtttt gccttatcca ccgcgtcttt tactttttgg ttagcatcat caatatctgt
     7321 tcctgaaaca aattccacct gaatgctgga aaagtcctga acagaattac tgataatttt
     7381 attgatgccg ctaattgctc caatctcttt ttccaattct ttggtaatga gattttccat
     7441 atcggcaggc gatgaacccg gataaattgt tgccacataa acagtaggca tggtgatatc
     7501 cggatataat tctttcggca gcgcattata ggtagagata cccataatgg tgagcattat
     7561 cgtgataata aaaatggttg ttttattatc aattgcccag ctcgaaggtt taaattcctt
     7621 gaatacgtct ttcattgttt aatatttttt attgccgtgg gtaaaataat ttactttaag
     7681 caaccctgat ttatcaatat ttaatttcct gaccagcaat taaattctgg taaccgcttg
     7741 taattacttt atcccctttt tcaagtcctg aagtaatttc agccatacca ttataaattt
     7801 tacctacggt tacaacgcgg cgctgtgcaa tagatttacc attttcctca gctgcgacat
     7861 atacaaatga tgcatcagtt gatttttgaa tcatgtttaa atccaccacc attgcattag
     7921 ctacttcata atcggcaatt ttaataacgg ctatcatatt cggttttaaa ttactttcag
     7981 atggcaattt agcttctaca gaaaatgttc tgctttgcgg attaattaca cttgaagcaa
     8041 atgaaatttt tgcgtcaatt tctttattta aatcagtcaa aataatttta gtattatcac
     8101 ctgctgaaac aaattctgca tgtgattccg atacttcacc ttttgctttt aaggtgcgca
     8161 tatttactac acgaataccc tgaaatcccg gagaaccaat ttgtccaacc tttacatcaa
     8221 ccgcatctac aacacctgaa attggtgaaa ttaatttggt tgcgcttatt tgttcgtttg
     8281 cagccgcaat acttttttct aaagtttcta cattgttttt tgcagataag tattgaattt
     8341 cgctaccaat tttttggtcc cataatgctt tttgtttatt gaacacatcc ttagcaaatg
     8401 ctaattgtga ttgtaatgtt tcaatgtttt ttaccatagc atcattatca gtttcagcca
     8461 atacttgtcc ttttgatacg ctttgaccaa ctacaacata aacagcatca ataacaccgg
     8521 gaatttgtgg cgtaactata acattctcat ctgcatcaat ttttccttgc agttcaatat
     8581 aatgggtaaa agggattggt tgaatttcag taaccactac taattcacct ttatctgatt
     8641 ttgtagtgtc atttaatgcc agttcttttt caatggcttt aattttatca tcgagtgcag
     8701 agcgctcttt tttaagtgat tccaattcag cttgtttgtc gccagattct ttagcgccgc
     8761 atgaagcgag gaaaacaatt acggttaaat ataataaggt ctttctcata gtatgtaagt
     8821 tttaataata tcctaatgat ttgtttaaag cagtttttgc attgagcata ttaaacatag
     8881 cgctcacata attgccctgc gcttggtata atgtactttc agcactattt aattcaagac
     8941 ttgatccaac accttcgcga tacttaatta aagcagtatc ataaatgcgt tgtgcaagtg
     9001 caatattttg tgtttgcacg ttcaggtcgt tttcagcatt gatataatct gttttggctt
     9061 tttcaatttc caataaaata ccttgcgtgg ccaattgctg ttgaatacgt gcttggtcta
     9121 aatcgatttg agcagcttga agaccggttt ttctctccat acctgaaaat acaggaatag
     9181 aaagtgttaa cccgaataat cctgtttcga accattcctg atcaccatct aaaaaatcga
     9241 aactatttcg ttgtgcattt tgcgagtatg aaccataggc tactaaagtt ggtaaaaaca
     9301 atgccttgtt catttttaca ttcaattcat ttaatgtaat tgtactttgt aaaatgctga
     9361 attccggtct cgacaaataa tttgttccaa ccatattcac ttctgtcatt gcgcctatcg
     9421 aatcggttaa ttcaatattg gttgccatgt ccaatcccat cgaaaatttc aaatagttgg
     9481 ttgccaattg tgtttgtcgc tgcgcatttt ccaactgacc attcaaggta agtttgctaa
     9541 tggtaagacg gtcaacatca ataccctcaa caaaaccttg tttttgcatt tcggtaactt
     9601 caaataaaac agcatccaga ttttcaatat ttttttgcaa taccttttca aattcttttg
     9661 caattaatac tgaataataa gctgtttcta catttttttt cacctcggtt tctgcaagtc
     9721 tggtttgttt ttgcaccaat gcagcatatt cttttgctgc actaatacca actaaccatg
     9781 caccgttgaa taataattgc gaagcagaaa tctgggctgt catgttttgt tctgttccaa
     9841 actgaacagg aataaactga cccggttcac ctccaaaaaa ttcagcggga attaatgatg
     9901 tgggtaattt gaaaaagttt tgataatcga gcgatgcatc aatttgtggt aatccgctag
     9961 cgatgcgatt gttcaccgta atttgcgcat cctgctcatc cagcgccgcg gataaaattt
    10021 gagcgttgtt tttaatgccg tagtccattg cttcctgcaa cgaatattgt tgcgattggg
    10081 caaacaaagc aaatggaacc accagggcaa ttaaaaacgc tttgagttta tatgccattg
    10141 ttttctgatt ttattttttc taagtattta attcctttgg ttgttgctaa tccacggata
    10201 tgataatcca tgaatacatt aaatgttttt tcgggaggaa atttatcata tgggaatatt
    10261 tctgcatcta aaaacatttc cattcttgcg atataaattc tggctaacac ttctgtatcc
    10321 aattcttcgc gatacaatcc ttcttttatt ccacgttcca aattgtgttt tatacaattg
    10381 taaacttctg tattcctatg gtcgttaaaa atttgccagc tttgtggatg atactttttt
    10441 aagtcaaata ccaaacttgg atttaaattt ttgctcatcg tattcatcca attggaaatg
    10501 ccaatcattt catcaatcgc tgttttggaa tgatgaataa tgtgctctac caccttgttt
    10561 tgacaagtga aatggttttg agtaaccttg tgtatcagct cctctttatt ctcgaaatac
    10621 tgatacaagg tctttttact catgcccagt tcacgcgaaa catcgtccat ggtcacgctt
    10681 ttaatgccat atcgcatgaa cagacgctca catttttcta atattttagc ttcttcactc
    10741 ataatttggc cgcaaaggta aatacacgaa actcaggaaa ctaaattttg ttcaaaagtt
    10801 tccaaagttt tttagataat ttaacatttg ggaaactttc agcgtttcca aaggcaaagt
    10861 tggaagtcaa aagccgcttt acgacaaagt tttagacgaa cgggaggttt ttagggatga
    10921 aacgtagata tttgggttgt acgggaaacg ggttattgtg gcagaggaat gtccaatcct
    10981 gatattccag gattatacca accggctaac caaggtgtcc gcaagtttgg tttgggtaga
    11041 tgtaattccg gataaaacat taactcccgg gcgtttccct ttttcgaagg tgccatattg
    11101 gttgtttata cccagggctt tagctccgta ttgggttgcc caacgcagca aatctgatgt
    11161 tggcaactcc gggaatttgt tttgtatggt ttgaatttcg cttaataccg acaaaccatg
    11221 attagatgca aggctgtcgg taccaactgc aatatattga acatgttgca tgagcatagg
    11281 aatatccggt aacctgtctt ctataaataa attagccttg ggacaaaaac accaaaataa
    11341 attgggatga atatcttctg cccactccgc atcccatgaa gttgcaatgg tattatgcac
    11401 taataacgta gatacttctt tattaaaata gggtaataaa ctttctaatg cggtttttcc
    11461 tgttggatta aaatgtttga tgtctacacc caattctctg tataatttgc gatataatcc
    11521 tgaagcttgg atataaaatt cattttcatc attactttcc aacatgtgta tggaagtagg
    11581 ttttccattt gcgtaacaac tggcatttat acttttaatt aaatcgggac taacactata
    11641 agtggcatgt ggtgctaaag aagcatgtat gccgtatcct cttgctgcag ataataattg
    11701 ttcaccattt tgcattgcac catttgcccg agcgggatca aatgcgatta attcaataaa
    11761 ggtgtgataa tataaattgc gtttagcttt tatataaaat gaattgctgt tattagagat
    11821 gtcaccaacg gctacaatac cattggttat catttcgttt tcagccaact ccattgcttc
    11881 attaattatt tcaatgggaa catttctttg ttgagttact aaagataaaa attgtggcag
    11941 acctgttttt tcgggaaatt tatttttgag atgagatagt tccaaatgac agtgcgcatt
    12001 aacaaacccg ggcactaacg caccactcac tttttcaata gaagcgttat ccaattcagt
    12061 tattggtaaa atgtctgcaa cagtgccatc atcttctaat actaacacat gattggttag
    12121 tggttgagta tgaataggaa aaataatatc cgcagcaatt ttccgcatat aaaaaaatta
    12181 accatttgct gtaaaaacaa atggcaatag attttaatta attttaaaac ttaatccaac
    12241 tcgtactgct ccactaaatg tatttaatga agcatcccaa ttagttaaat caaaagtttc
    12301 gccatcaata taatattgtt tcaaagtaaa atcattcctt gtatattctg cttgagcaaa
    12361 tacacctgca aatgaaaata tataccaatt gaaaccaaga tttacgccaa aatgtccgcc
    12421 gctataatta gatacttctt caatatttcc caaacttgga ttgagttttc gtatttcaag
    12481 aaaagaataa cccacttccg ggcctatgta taaattaaat ttttctccgt ttacaggata
    12541 taatcttaaa tcaaaagcaa tagtgccgac attatttcca ttagcactca catctgatgt
    12601 gtcttctaaa tacgagccgt acacaaaggt agcgccgccc gacaaccagt ttaataaatt
    12661 atattgtgcg ccaaaactaa acatcgacgt tgctgcacca ccggtatcag tttcaagtgg
    12721 caacccaagg tattgcgatt tatataaggt tccatgaata ccaaaatcgt atcctgcctg
    12781 aaatgataat gttcctgcct gttgtccctg tccgaaaatg gtaacacatg aaatcaggga
    12841 catggaaagc acaaataatt taactttcat ataatgaaat tttgataatg aaataaaaat
    12901 ttaaggaata aaaaaaactt caattataat gccacaggaa ttttccgatt ataaaaacaa
    12961 atccattaat aattaatttc ctgttccaca atattttccg gaatagccct ttcctcatat
    13021 tgctgattac gcaattgtgg ttcaaatccc gcttcgcgaa tagcatcctg aatagtttta
    13081 tatgtaaacc tgtgtggtgc tcctgctgcg ctcacaacat tttcttcaat catgatactt
    13141 ccaaaatcgt ttgcgccggc gtgtaaacaa atttgtgcta catttttccc tacggttaac
    13201 cagcttgcct gtatgttttc aatattaggt aaaaacattc tgcaaatggc aattgtgcgg
    13261 atatattcat caggcgtggt gtcgtttttc gcacggcgta tttttttaag cgtggtatcc
    13321 acatcctgga aagtccaagg tataaatgct aaaaatcctt ttgcatgtgc gggcttttca
    13381 ctttgtactt tacgtatttt ttccaaatgt aaaaatcgct cttcaatggt ttcaatatga
    13441 ccaaacatca tagttgcgct cgtggtaata tttaatttat gtgccacacg cataacattt
    13501 aaccactcat cggcagtaca ttttccggta gaaataattt tccgaacacg gtcaactaaa
    13561 atttcggctc ctgcgccggg taacgaatca agtcctgctt taaccaactc cgttaataca
    13621 tgttcatacg tgtggttgcc taaagaagca atatgatgaa tttcgggcgg accgagtgag
    13681 tgtaatttaa tttgaggaaa catttctttt atttccctaa acgttttagc ataaaattca
    13741 agtcctaaat ccggatgatg tccgccttgt aataaaagct gttcaccacc atatttaagt
    13801 gtttcttcaa ttttaatgcg atatgtttcc ttatcggtga tataaccttc agcatgcccg
    13861 ggaaccctgt agaaattgca aaatttacaa ttagccacgc aaatattggt ggtatttagg
    13921 ttgcggtcaa taatccaggt aaccttatta cccggttttt tgcgcctgcg aatggtgtct
    13981 gcaacctgca ttaattcagc agtaggtgcg ttttcaaaca aatacatccc ttcctctacc
    14041 gaaagaaatt caagatttaa ggctttttga taaagcttgc ctagttccat attcacaaag
    14101 gtaaaacaaa gaattatgga ttgggttcta taaaatttga aatggttgaa attcaggact
    14161 ttaaaaaagt tataactcaa aaacagaaaa cgtattatat ttgcattccc taaaaacaac
    14221 gggcctatag ctcattcggt tagagcaact gactcataat cagtagtccc gatagctatc
    14281 gggattggtt cgattccaag tgggcaagtt ccaaccaatc aagtggtttt tacttcgcag
    14341 tctctaattt aaaattagac cgtttcgata aaaataaaac gtattatatt tgcattccct
    14401 aaaaacaacg ggcctatagc tcattcggtt agagcaactg actcataatc agtaggtgcc
    14461 tggttcgatt ccaggtgggc ccacaactag caccttaggg tgcttttttt gtttatgcct
    14521 ttttatacat atattttatt tagcaaatca ctcaataaat tctacattgg ttccaccagc
    14581 ctgagccctg ttgagcgact tgaacaacac aatcaaataa aatttcctga tgcatttaca
    14641 cgcaaaggaa ttccttggga gatgttttta gtaattgaat gtaattcttt tatccaggct
    14701 aaagctattg aacttcacat caaaaaaatg aaaagtacca gatacttgca aaatctaaaa
    14761 caatatccag aaatcggaga aaatttatta cttaaatata gagcaactga ctcataatca
    14821 gtagtcccga tagctatcgg gattggttcg attccaggtg ggcccacaac ttgcacctta
    14881 gggtgctttt tttgtttatg cccttttata ctttcattta attagcccaa aactcaacaa
    14941 tttctacata ggagctacca gcatttatcc taaattgagg ttggaagaac ataatcaaat
    15001 caaatttcct gatgcattta cccgtatatg aaccatggga tttacttttt atgatggttg
    15061 tcacaagttt aaacaggcta aggctattgt attacacatt caggttataa atagttaaaa
    15121 ctttccattt tatactgaaa tatgcatgtt gtttatttat attttgtaaa ttcacattat
    15181 cctatagtat tttaatgaat aaaacctaaa taaaatatag ttttttcaca ttagattcct
    15241 tcaaatgaga cctctaatta cctcaaaatc agtagtttga aatttaaaaa aacatgtaac
    15301 gttaataatg gtatagtaat tttcaccatt ttgctaactt tcaaaaccta cctttaatag
    15361 gttcgacatg aatgcagaac aatttgaaaa aataaaacaa ttatcaacaa aaaataatag
    15421 aataggtgta gttgctaaac ttattctgga agaaatttca agtgtgcctt acgaggaacc
    15481 actagaagaa atcaatttag aatcggatga ggattataaa gagcagtctg aaatcaacga
    15541 aacaggagcc attcaaatct accctcatcc ggtaggttcg caaagtgtta ttgaaatttt
    15601 gttatccgat ttaccagaaa attcaagatt tataatctac gatttgcatg gaaaagtatt
    15661 atttaattat gacttattac aacaatctac tttgataatt gtgaataatg atatgttaac
    15721 ctctggtctt tatctaggag tagtcacatc aaataatatg attaaacagt caataaaatt
    15781 tgtagtcaat gaataaatat tttaggatag tttttttaca aataagcatt ttattttttt
    15841 taccggaaat aaaatcccaa aacacctata atattttgat tgatcacgaa aaccaatcgg
    15901 aatggggtta tagtcttttg atggatatgg atactattgt tatgacaggt tatactaatg
    15961 ttttaaaaga tggattttac cgtgatggtg tgttttttag aaaattggat ttagaaggca
    16021 atatatttcg tgaaaattta attgccgata cttgtgtgct ttatacagtt ggttacccag
    16081 ggaaccttgt caaaattgat aatcagtatt taacgagtgg aatatatcaa tattgcgatt
    16141 acaattttga gggtggtgtt ggtagttttg gcatggtata caagttcaat gaaataggtg
    16201 atacaatttg gactaaaaaa ttcgacagtc aatcttattc aattatttat aaaggggcta
    16261 caacgacaaa taattcttat ctttctgcag gcgtatcggt tgataccgca acgttaatcg
    16321 ataaatttta tattattaaa atagactcca ccggggaaat agtttgggaa aatacttttg
    16381 gctatctgct taataattat gcattttcta tagattcata tacaaatggt gattttatca
    16441 ttggtggatt aacggaccca acaacaagtt tttcacagac agatgggtat ttagcaaaat
    16501 tagattcatc cggcaacctc atctggcaaa aaaaaattgg cactccagat gacgataatg
    16561 ggtgtattgc aagaattacc ttcgaccaac aaaacattta cctgcaacaa agtcttgata
    16621 cagttgtctc gattggtgat gcaccaatgc ccgattacgt gggtaaactt gactacaatg
    16681 gcaatttcat ttggcgaact ttttttaatg gaccctacaa gcaggatatt tggggtatac
    16741 gagaaatgga aaaaaaatcg attatggttg ttggaaccaa aatccctgat gatgtaagtc
    16801 agggcagtgg ctttatttgt aaacttgata gcaacggcgt taaactgtgg gaaagaacat
    16861 attatactag aacagacaga gataattatt ttaccgatgt ggcaaaagtt tcagacggag
    16921 gttatatttt aacaggtagt gcatggagtg caaccgatcg ggatgtatgg ctagtacgcc
    16981 ttgatagtat gggttgttta gaaccgggtt gtgatattgt tgatgtgcca acaataacgg
    17041 taaacaatac aacattattt aatttttacc ccaaccccat gcacaacacc gcaactgttg
    17101 aattgcaaat tccctataat tttcaaatac tatcaggtgc aacaattatg ttacaaataa
    17161 tggatgtaag ttgtaaaatt gtagatacct atgccaatat tccaataagt aatcccggtg
    17221 aaaccatccg gtttgatgtg tataggaaaa atttggcggc cgggtattat catgctttgg
    17281 ttaaatacaa caatgtaaac tttggtagtt ataaatttgt gattaattaa ttattataac
    17341 aaaagataca tttatatatt ttcgaaatta tcgttcatct taaatttgaa tatactttta
    17401 gttagtgtct aatacgggta taccccgcta tttccttatc catataatcc actttcatac
    17461 tttttcctat aatcaacagg cgacatacct gccgtttttt taaacacatc tctgaacgct
    17521 ttcaaatcgg cataacccac ctcaaacata acctcattaa ctgttttgcg acccgattcg
    17581 agcaaacttt ttgctgcttc tatttttact ctttgcaaat attcggcagg cgaattagat
    17641 gtggcctttt taaaacggcg ttcaaatgtg cggcgactta aattacttaa agcagctaat
    17701 tcatcaacgg taatcttgtt tttgtaatgt gtttcgatat atacctgtgc ttccagaata
    17761 acacaatcac catggtcttt ttgtcctttg aaaatggaaa atggggactg actatttaaa
    17821 ttaacattca gcaaaaaaaa ctttgaagcc attattgcca tctccttatt ggtgtatttt
    17881 tcaaccaaat acaataataa attccaataa gccatagcgc cgccgcttga ataaattccg
    17941 ttttgatcaa ctatcaccct gtcgtctgct aaggttactt ccggaaataa ttgtcggaac
    18001 tgattggcaa acatccaatg cgtggaacaa ctttttccat ctaataatcc ggtagaagcc
    18061 aacaaaaacg ccccaacgca taaactggca acttctgaac cgcttttata gcgatttact
    18121 acccatggaa ttaaatcgct gttgagtgct atagcagttt ccatatctcc gctcaatgcc
    18181 ggaattataa ttaaatctgt ttcacccact tcggttaaca gtaagtccgg cctgacacta
    18241 aatgaaccct gatttaacaa aatctcatgt tttgccgcta ctaactgcac tttaaatggt
    18301 atcggtaaac cggcagctcg gtgaaattca ttcacagcgc taaacatgta acgcgggtcc
    18361 actacggcag ccggcacagc cgtttcggga attaaaatcg atattgtttt cacatggcaa
    18421 aagtattaaa atatcttgtc gcaaacaacc ccttacattg tcgttttccc cctcttaaat
    18481 atgggtgttt gcgacataaa tttgtggcat ggaaaatcat actgaaatca ctacaatacc
    18541 taacgacata gtagtttgct gcatacgtgc agaatctttt ccgggtggtg ttttggcggc
    18601 acaccaggaa ttgcataggt taattccgca aaaaccggga agaatgtatt atggcatttc
    18661 ctatcccgat gtaaccagaa aaattttata taaagcagct gttaacatgc tgaattatga
    18721 tgaacccgaa aaattaaatt gcgaaacgga tgtaataaaa gccggcaaat attatagcac
    18781 aattttaacc gattttatga acaatattcc tgcaataagc catattttta atcaattatt
    18841 gcaacatccg gatttagacc ctgaaggtta ttgtttagaa atttatatga acgataaaga
    18901 tgtgcaacta ttagtcaaat taatttaatt caaaaaaaca aaaaatggca actcaaattt
    18961 ttattaatct tcctgtgaaa gaccttcaaa aatcaattgg ttttttcaca tatcttggat
    19021 ttacctttaa tccgcaattc accgacgaaa cagcaacttg tatgattatc agtgaaaata
    19081 tttatgtaat gttattaatt gaatcgcgct tccaggaatt tacaaaaaaa cctgtcagcg
    19141 atgccaaatc aagcacagaa gttctcattt gtttagacgc tgaaagcaga gaagctgttg
    19201 acaccatggt aaaaaatgct gttgatgcag gcggaaggat ttacgccgac ccaatggatc
    19261 atggctggat gtacggacat agtttcgaag acctcgatgg tcaccaatgg gaggttgcct
    19321 atatggatat caccgcattt cctcaaccag aaaataatta actatgattt caaatataat
    19381 tccttacatt aattttaatg gaaattgtga agcggccctc acattttatc aatcgtgttt
    19441 aggtggtgaa ataaaattat tacctgtaaa agaatcacct gctgcgccgc aattaccttc
    19501 agaaatgcat gaaaaaataa tgcatggaga aattaaattc ggtaacatga tgataatggc
    19561 taccgacatt aacgatcaac gctcacaaac aggcaatgcc gtgtcgttat ttattcattt
    19621 taccgaaaaa gatgaattaa tgcgtgcatt cgaagctttg tctgccggtg gagttatcac
    19681 acttgcagtg gcaaatcaat cgtggggagc tacctatggt gagcttatcg acaaatttgg
    19741 catcaagtgg cgatgcaatt ttcatcaaat gtaaattgaa cactttttag agcgacttaa
    19801 tagtaaaaat attaagtcgt tttttttgac gtttaatcta gaacagccgt agcctcaatc
    19861 tccaccaaat attcaggagc aatgagtttg ctgatttcta ccatggtagt acatggtttt
    19921 atatctttaa aaaactcgcc atggcctctg ccgtattct
//
LOCUS       DAORKW010000013        19705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_22_length_19705_cov_4.707837, whole genome shotgun sequence.
ACCESSION   DAORKW010000013 DAORKW010000000
VERSION     DAORKW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19705
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22_length_19705_cov_4.707837"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..662)
                     /locus_tag="PLB46_01250"
     CDS             complement(66..662)
                     /locus_tag="PLB46_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF75165.1"
                     /translation="MSDFLSADYWEERYKLHDTGWDMGEVSPPLKAYFDQLTNKDVAI
                     LIPGCGNSYEAEYLVQQGFTNVTVIDIAEGPVERLNAVLGEEGRKCCRVIHADFFKHE
                     GQYDIVVEQTFFCALDPQLRKQYVEHMFTLLKPGGKIAGVMFKTHFEKPGPPFGGTQE
                     EYIALFKDKFFVHTMAGCYNSHEKRLGNEIFVIFKKLS"
     gene            complement(662..1297)
                     /locus_tag="PLB46_01255"
     CDS             complement(662..1297)
                     /locus_tag="PLB46_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF75166.1"
                     /translation="MDDIKEGLLKRFPHFEPQLIDKIAEASTMRTFNRDEVIMHAGGY
                     FKTTILVVDGLIKLYREGDDGGEYFMYYLEGGEGCALSMICAARNKASEVTGVAVEDT
                     VAILIPLELMDILMRDYKTWYYFVVQTYRSRFEDLLNVIDQIAFKNMDERLLNYLDGQ
                     FGEFNTTMLQITHQQIASDLNSTREVISRLLKKMEQNGVIKLHRNAIEKIK"
     gene            complement(1312..1566)
                     /locus_tag="PLB46_01260"
     CDS             complement(1312..1566)
                     /locus_tag="PLB46_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75167.1"
                     /translation="MSINSFFDNWKYFLLFCLTLGLAPFAPEPHIVGKIRWIAGGAVG
                     MTGMDWFDVVLHGTPWVLLIISGGGALFRYFKQKGNNSEI"
     gene            complement(1556..1867)
                     /locus_tag="PLB46_01265"
     CDS             complement(1556..1867)
                     /locus_tag="PLB46_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF75168.1"
                     /translation="MFDFIKKLFGGRTVNYSELIENGAIIVDVRTPQEYRSGNIRGSL
                     NIPLDQLKGNLKKIKKDKPVIVCCASGMRSGAAKGVLQSNGFTQVYNAGSWHKLTKYV
                     H"
     gene            complement(1877..2152)
                     /locus_tag="PLB46_01270"
     CDS             complement(1877..2152)
                     /locus_tag="PLB46_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF75169.1"
                     /translation="MTLETILKSPTVSIVDVRTPAEFMGGHVADSINIPLNEVPQNLD
                     EFKNMAKPIVLICASGMRSAQATQYLASQGVEQCYNGGSWLVVNGLV"
     gene            complement(2253..3101)
                     /locus_tag="PLB46_01275"
     CDS             complement(2253..3101)
                     /locus_tag="PLB46_01275"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRF75170.1"
                     /translation="MPRSLFVFMLVLGLGLTACDQKKDFTDKSLEPSEVEILNAYGNK
                     VVSAQQTTQQPAAPTSTYASERKVIKKGNISFTSDNLDATEVVIKTALSANGGYISEE
                     SSNENDYNASRKMIVRIPAAQFDVFLKDISNKAGKLESKNITTEDVTTEFLDLTAHIR
                     IKQALEERYYELLQQCKTMDAIIQMEKQLNEVRTEIETAQGRLNYLSGLTTYSTLTIS
                     FYTPNTYIAEKPTFGTKIKTAFSNGWIFILQFIIAITSIWPLFIFILIGWYIYRLIKR
                     KLVIIK"
     gene            3280..5670
                     /locus_tag="PLB46_01280"
     CDS             3280..5670
                     /locus_tag="PLB46_01280"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF75171.1"
                     /translation="MHKTFTQPQFVLSQFFNFPKTNFVHYLLLAILLLNTGLPIHAQA
                     PEIEWAKSYGGSGSEIAHKVLLTADGGYYVVGYTSSNSGDVAFNNGSMDYWIIKLNSA
                     GDILWENTYGGSNYDYALSAVLTPDDGCILVGKSQSNDGDATDNHGFDDYFAVRVDSD
                     GNTIWKKSYGGSNFEQAKAIVATNDGNYVMYGYSYSTDGDKSTTYGGADIWLVKINPD
                     GDIIWEKSYGDAGENFGDAIVQSSTGDFVLTWHIAGAGDYRLMKLSADGDIIWNQTYG
                     GTNNDIAYAIKENTSGGFIINGQSNSGDGDVVGLHGVASDMWAMEINGDGAIIWKKCF
                     GGSADEIGNDVIINNSGNYVLAGIATSDNDGDVVGHHGGAYSGDYWVVELDASQNIIW
                     QLSLGGTSTDELNTIQQTPDNGYIAAGWSASANGDVTDHIFIFDFWVVKLSNNCEELT
                     WYADADNDGFGNILSMLTACDMPSGYVANADDCNDANAGIFPGNTDICNAVDDDCDGL
                     TDEDVVFTTYYFDNDADGFGDTDMDTITCSSIFGYVLDNTDCNDTNASINPDATEICN
                     DLDDNCNGLSDEGLPEYVLFADADSDTYGNADLYITSCYAAIIGYVVDATDCDDSNAD
                     INPAADEICNSLDDDCDGYTDENLSFIIYYEDADSDTYGNADIDSLACLTPTGYVANN
                     TDCDDTNAAIYPGATEIFNGIDDNCDELIDEGVAVHELSPDAIMHINNPVTTNLQINY
                     SGKESIDFYIITITGENVYKGQLITGENNLDMSAFSNGLYFVVTNNLKVVRFEKIN"
     gene            complement(6331..7254)
                     /locus_tag="PLB46_01285"
     CDS             complement(6331..7254)
                     /locus_tag="PLB46_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75172.1"
                     /translation="MAILPLENSLNSRLKKSKFHIKEYSAFAASFLLIKNDVQAQVFY
                     GDIIPDTLINEDWETYTVDLNNDGLSDFRFLKRSFWFMPTSSSTSHFSAIYVGPLVFG
                     NLIAGNPHVIFPSYGGSTVYHPYALMEGTIIDQESLDFQYAGYQELAYRFLGENSTYW
                     PHGGHWYPEVTDRYLGVYFKDTANCYHYGWIRLDVKDFGRELVIKDFAYETECDHPIV
                     AGDTISYIDVADENKLDAVVYSFDKTIYVKVSELSNDLQISVADITGKEIFSNSIYNL
                     NSSFNIEQPSGIYTVSIISDEKRYSKKVHIN"
     gene            complement(7774..8141)
                     /gene="ssrA"
                     /locus_tag="PLB46_01290"
     tmRNA           complement(7774..8141)
                     /gene="ssrA"
                     /locus_tag="PLB46_01290"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            8262..9959
                     /locus_tag="PLB46_01295"
     CDS             8262..9959
                     /locus_tag="PLB46_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRF75173.1"
                     /translation="MADQQDTSQSTTSPYKRIIPDIEDWPIHIISKNREQVIKSVVEE
                     TLQNIINKNATEKDVHNELAKAIYLEKIRVERPWKTDPPDDKKFWTEVKNKLVEFESH
                     PDGNIEAEKEILRRIITRYVTEIVGNFNKGTYQFSERFVPFAFNRLLNASHSRAFVRL
                     FGNKKDLLKSIHVIGEIDQLRNLAKDGIVVVVPTHTSNIDSITVGWGISAIGLPACFY
                     GAGLNLFSVRFLAYFMNRLGAYRVDRRKKNAVYLEALKTYSTQTIYQGGHSLFFPGGT
                     RSRSNMVETQLKLGLLGTALEAQRRQIINTNGEGFKKVYVVPVTLNYGFVLEAPELID
                     EHLRNVGKERYFVPSDKYSSSYKILKFLYRFITADADFTIAFGRCMDIFGNPVDDNGN
                     SLDQNGNVIDIRDYFKSKGELKYDTQRDAVYTKMLSEGIVKAFKRNNVALPEHVIAYA
                     AFKLFQQKHHTSDLYSVLRMPEEFRELSKDELREAVRKVVKELTKLEHKEEIRVDPIV
                     KAEDTDELIDYAVKKLGVYHAKRPLLKTKKGMIKCQDMKLLLFYHNRLEGYGLEKHV"
     gene            9952..10995
                     /locus_tag="PLB46_01300"
     CDS             9952..10995
                     /locus_tag="PLB46_01300"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRF75174.1"
                     /translation="MSKKYSVGVVGAGSFGTAVANLLAENNHVYLYSRRAENVDEIIR
                     SKTSSNQKIHENITPTNDIGLLTRECYLIFPIVSSDGFKDMIRTMAPFLRPDHILIHG
                     TKGLAVKKKGLDKLKQRARIAGKNVLTMSELIRSESVVVRVGCMAGPNLAKEISAGLP
                     AATVIASRFDEVIHEGQMALKSPRFQVYGSYDIVGVELAGVLKNILAIGSGIVSGLNL
                     GENARALLITKGWAEIIKLSEALGADVKSFLGLAGIGDIIATCSSPNSRNYSVGYRLA
                     KGETITDIKNTMEEVAEGINTIRIANGLANYYKVNCPIIKTLAQGIFEELDVQAGIEY
                     LMKYRMSMDVEFI"
     gene            complement(11032..11220)
                     /locus_tag="PLB46_01305"
     CDS             complement(11032..11220)
                     /locus_tag="PLB46_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75175.1"
                     /translation="MEKLIQMKLSGSWNEVIFIPTFLFRTHFKNRFVLVFSSIRSLIE
                     AIDAAHTGGSTGNSSNEG"
     gene            complement(11208..11810)
                     /locus_tag="PLB46_01310"
     CDS             complement(11208..11810)
                     /locus_tag="PLB46_01310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF75176.1"
                     /translation="MFSILIPAIAALLSLQPQSATGYAVGDEVKNFSLTNIDGKNVSL
                     NDYASREGVIVVFTCNHCPYAKAYETRIMELDKNFASKGYPVLAINPNDPIREPEDSP
                     ENMKKRAEEMAYSFPYLFDATQQVAKDFGATRTPHVYLLKNNNGKFTVEYIGAIDDNT
                     DEPAAVKTKYVEDAIGALKKGEKPAVNFTKAIGCTIKWKS"
     gene            complement(12103..12582)
                     /locus_tag="PLB46_01315"
     CDS             complement(12103..12582)
                     /locus_tag="PLB46_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF75177.1"
                     /translation="MKWLCTLILFVAMQVVAAQRVEYKTFAEVDSFFETSNDTLYVVN
                     FWATWCKPCIEELPSFEKINAEYKNQKVKVILISLDAQMRWESSLIPFLQTNNYASSV
                     WAIYKDRPEDWIDRIDKRWQGTIPATIMFNKQRGISFIHETEITYEQLDQKIKEFIQ"
     gene            complement(12632..13132)
                     /locus_tag="PLB46_01320"
     CDS             complement(12632..13132)
                     /locus_tag="PLB46_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRF75178.1"
                     /translation="MKKIFHLAFAFTIVLFVSSCKDEKPQPKKPATLTDGQPKFTKEG
                     ELDFFKPDGTKVTHINIEIADDDSQRQQGLMNRSFMNNDQGMLFIFDKEERQAFWMRN
                     TILPLDIIYVNAKKEIVSIAENAEPFSEQSLPSKGPAIYVVEVNAGFCVQYGITAGCK
                     IDFTRN"
     gene            13290..14087
                     /locus_tag="PLB46_01325"
     CDS             13290..14087
                     /locus_tag="PLB46_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HRF75179.1"
                     /translation="MTIIEDPNKPKTPVTEERRPLEHRRYYLTGLALLAAGILFFLDR
                     STDYDLPFPLISWEFMLIIAGIYVGEKSSFTGLSWIACIAIGGYFLLDDFFPHFDLRF
                     YFGPLFLIGLGIYFLAGPRTFRYRRSRAGRNKRYENYRNTVEKGFASENSLSDDYIDI
                     VSIFGGVQKQVTTKQFKGGEAVSIFGGTEINLMQADFEGKVILELTQIFGGCTLIIPP
                     HWELKSELVSVFGGSEDNRPSTTVTDANKVVILKGTSIFGGIEIKSY"
     gene            14096..15136
                     /locus_tag="PLB46_01330"
     CDS             14096..15136
                     /locus_tag="PLB46_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF75180.1"
                     /translation="MATNPLFTSKYIKAASLSLFIWTVLHASFIYWLGFDIKIALADA
                     AAFNFLLADFCLAQLFVMQFYQPKKDQTAYIIVAGTLIATSVTFLSRFLLGLFNFDET
                     YYTFIDTSIPVRIGVAFLFVAIINMFSMLVKGLEEQKTNSARAFEAEKLSKEAELYNL
                     RSQLQPHFLFNSLNSISALAGSKPEQARKMVQQLSDFLRGTIKKDNLQLIPLQEELHH
                     LDLYLEIEKVRFGHRLETAIVCDENCQTKKIPSLLLQPILENAIKFGLYDTLENVQIV
                     ISASCDENYLSISISNPYDQTSSPPKKGTGFGLSSVQRRLYLLFSRNDLLDTKQTDNQ
                     FITTVKIPQPKL"
     gene            15133..15867
                     /locus_tag="PLB46_01335"
     CDS             15133..15867
                     /locus_tag="PLB46_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF75181.1"
                     /translation="MMKVIIIDDEPLARSIVREYLEQHQDIDIVAECGDGFEGVKAIT
                     QHEPDLIFLDIQMPKINGFEMLELLDKRPSVIFTTAFDEYAIKAFDIHAVDYLLKPFN
                     KERFDKALERWRAMQPTTDKTPELLNTIDEQEQHPQRVVIKLNNKIKIIPFADIIYIE
                     AAEDYVFIHTSEGRFMKHKTMTFFEHALDKNIFVRCHRSFIVNVQLINRLEVYEKDSH
                     VAILTTGAKVQVSKSGYQRLKAVLGV"
     gene            complement(16106..17695)
                     /locus_tag="PLB46_01340"
     CDS             complement(16106..17695)
                     /locus_tag="PLB46_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75182.1"
                     /translation="MRFVLLLLVSVLVQQMFAQTYCRSYGDVGTEYSGKSIQTSDGGF
                     LITGNTNSWSGDFFDFYIVKTDSAANQIWSTVVYTEMEDIAKAAIELNDGSFLIAGSA
                     YTEVGGYDVALVQLNAEGELITSKFIGDETSNIGNQLIKTIDGGYVIVGYTDTVGAAL
                     ITYQPMILKLNAALEVEWSRRFTVCGSCSGEAMDVVQLQDGNLIVTGYLHTGFPVTTG
                     DMFVAKFTSAGALSWFNRTGKADKFESGNSIVAVNSDKFIIAGSSFEDNYDIFLSEID
                     GDGNTQWQKIINSGTSEMTAQIIQTHDNQYAIAGSKNSGEDDYYLLKLDTAMQIVFSS
                     FAGQDEIEAASGLLETKSNHFLLTGHGIPNGELYDQIYVLQFDDKGYNCSKTDSGGTV
                     EVYDGVYNLGGFAMPITFSIDTGAIWFSGAGFTTICADTATQDTSTISLEETNSFDNC
                     RMYPNPAASFVEINNLQTGLFTTNLKVVNSSGQLVYETVIPKGQTSCSIDVSGLPNGL
                     YLVNINNGYNYKVFQLVVTHN"
     gene            complement(17802..19436)
                     /gene="groL"
                     /locus_tag="PLB46_01345"
     CDS             complement(17802..19436)
                     /gene="groL"
                     /locus_tag="PLB46_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRF75183.1"
                     /translation="MAKIIKFDVEAREKLKAGVDALANAVKVTLGPKGRNVVIEKKYG
                     APNVTKDGVSVAKEIELEDPIENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIVTAG
                     LKNVAAGANPMDLKRGIDKAVEAVVIELKKQAQQVGNDNKKIEQVAAISANNDAEIGK
                     LIATAMQKVSKDGVITVEEAKGTDTYSDVVEGMQFDRGYLSAYFITNGDDMEAEMDRP
                     YILIYDKKISTMKDLLPILEKVAQQGSQLLIIAEDVDGEALTTLVVNKLRGTIKVCAV
                     KAPGFGDRRKEMLQDIAILTGGTMISEEQGKMLESVELGDLGRAEKVTVDKDNTTIVG
                     GKGKKDDIKIRVNTIKSQIETTTSDYDREKLQERLAKLSGGVAVLYIGAATEVAMKEK
                     KDRVDDALHATRAAVAEGIVAGGGVAYIRAIEVLEKLKVSNEDEKIGVDIIKRALEAP
                     IRTIVANAGIEGSIVVQKVREGKADFGFNARTEKYENLLKAGVIDPVKVTRVALENAA
                     SIAGLLLTTECTIVDKPEKKDMPMGGGMPGGMDGMM"
     gene            complement(19486..>19705)
                     /locus_tag="PLB46_01350"
     CDS             complement(19486..>19705)
                     /locus_tag="PLB46_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRF75184.1"
                     /translation="ETVTKGGIIIPDTAKEKPQKGIVVAAGPGKKDEPVTVKVGDSVL
                     YGKYAGTEITVDGQEYLIMREADIFAIL"
BASE COUNT         6082 a         3781 c         3538 g         6304 t
ORIGIN      
        1 tttgtgccat accttttata acacacaaaa ttcgttattc gttctatcca aatttgtgac
       61 ttttgtcacg acagcttttt aaaaatgaca aaaatttcgt tgccaagtcg tttttcgtga
      121 gagttgtagc aacctgccat ggtatgcaca aaaaatttat ctttaaataa ggcaatatat
      181 tcttcctgag tgccgccaaa cggaggaccc ggtttttcga aatgtgtttt aaacattacg
      241 ccggcaattt tacctccggg tttcagtaag gtaaacatgt gttcaacata ttgcttgcgc
      301 aattgcgggt cgagcgcaca aaaaaatgtt tgttcaacaa caatatcata ttgcccttcg
      361 tgtttaaaaa agtccgcatg aattacacgg caacatttac gtccttcttc tcccaaaaca
      421 gcatttaaac gttccaccgg accttcggct atgtcgataa cagtaacatt ggtaaaccct
      481 tgttgcacca gatattcagc ttcatatgaa ttaccacaac ccggaataag tatggcaaca
      541 tctttattgg ttaactggtc gaaataggct ttcaagggag gagaaacttc acccatatcc
      601 catccggtat catgaagttt atatctttct tcccaataat ctgcagataa aaaatcgctc
      661 attattttat tttttcaatt gcatttctat gcaacttaat tacaccattt tgttccatct
      721 ttttcagcaa acgagaaatt acttctctgg tagaattcaa atcactcgca atttgttgat
      781 gtgtgatttg cagcatcgta gtattaaatt ctccaaactg accatccaaa taattgagca
      841 accgttcatc catattttta aatgcaattt ggtcaatcac attcaataaa tcttcaaaac
      901 gggagcggta agtttgcacc acaaaataat accaggtttt gtagtcgcgc attaaaatat
      961 ccatcaattc gagtggtatt aaaatggcaa cagtatcttc aaccgcaaca ccggtaactt
     1021 cactagcttt gtttctggct gcacaaatca tagataaggc acaaccttca ccaccttcca
     1081 ggtaatacat aaagtattca ccaccatcat ccccttcgcg atataatttg attaaaccat
     1141 ccacaaccag aatggtagtt ttaaaataac caccggcatg cataatcacc tcatcgcggt
     1201 taaaagtacg catagtacta gcctcagcaa ttttgtcaat tagctgaggt tcaaaatgcg
     1261 ggaaacgttt gagtagccct tccttaatat cgtccatagg acaaaattaa atcagatttc
     1321 agaattgttg cccttttgtt tgaaatacct gaataatgca cctcccccac tgataatcaa
     1381 caaaacccaa ggcgttccgt gtaataccac atcgaaccaa tccatacccg tcatacctac
     1441 agcgccaccc gcaatccagc ggattttacc tacaatatgc ggttcagggg caaaaggcgc
     1501 caaacccaat gtaagacaaa acagtaaaaa atatttccag ttgtcgaaga agctgttaat
     1561 ggacatattt agtgagttta tgccaactgc ctgcattgta aacctgtgtg aagccattac
     1621 tttgcaacac tcctttagct gctccactgc gcatgcctga agcgcaacaa actataaccg
     1681 gcttgtcctt tttaattttt ttcagattgc ctttcaattg gtctaatgga atatttaatg
     1741 aaccccgaat attgcctgaa cggtattcct gtggggtgcg cacatcaaca ataatggcac
     1801 cattttctat gagttcactg taattaacag tcctgcctcc aaataatttt tttataaaat
     1861 cgaacataaa aatattttaa actaaaccat taactactaa ccaacttcca ccattataac
     1921 attgctcaac accttgagat gccaaatatt gtgttgcctg tgcactgcgc atacctgaag
     1981 cacaaattag cacaattggt ttcgccatat ttttaaattc atctaaattt tgaggcactt
     2041 catttaatgg aatgtttatt gaatcagcaa catgtccgcc cataaactca gccggcgttc
     2101 ttacgtcaac aatacttact gttggtgatt ttaagatagt ttctaatgtc attttcttta
     2161 atttttagca aaagtaacag taccccaaag tggagttggt gacaaaggtt actttgaaaa
     2221 atatttgcta aaggttatga caaattagcc tattatttta ttatcaccaa ttttcgtttg
     2281 attaaccgat atatatacca accaattaat ataaaaataa ataatggcca aatgcttgtt
     2341 atagcaataa taaattgtaa aataaatatc catccgttgc taaatgcagt tttaattttt
     2401 gtaccaaatg ttggtttttc agcgatatag gtatttggcg tataaaatga aatagtcaat
     2461 gtggaatatg tggtaagccc tgataaataa tttaatctgc cctgtgctgt ttcaatttct
     2521 gtgcgcactt cgttgagttg tttttccatt tgaataatgg catccattgt tttgcattgt
     2581 tgtaagagtt cataataacg ctcttccaat gcttgtttaa tacgaatatg cgcagttaaa
     2641 tctaaaaatt cggtggtgac atcttccgtt gtaatatttt tcgattccaa ttttccggct
     2701 ttattggaaa tatcctttaa aaaaacatca aattgcgcag caggaattct cacaatcatt
     2761 tttcgcgatg cattataatc gttttcgttg gaagattctt ctgaaatata cccaccgttg
     2821 gcggataatg ctgttttaat taccacttct gtggcatcaa gattatcgga agtgaaactg
     2881 atattccctt ttttaatcac ctttcgttcg ctagcataag tgcttgttgg agcagcaggt
     2941 tgttgtgtag tttgttgggc cgacactact ttatttccgt aagcatttaa aatctccacc
     3001 tccgagggtt cgagtgattt atcagtaaaa tcttttttct ggtcacatgc ggtaagcccc
     3061 aggcctaaca ccagcatgaa tacaaataaa cttcgtggca taataattgg tttaagtgaa
     3121 atagattgtt tgccaatgag atgcatggta gttcactttg cataagctca attgtaaatg
     3181 atttgtaaaa ttccaaacat tggaaatccg gtgtatggga gacgatgccg cctgctatcc
     3241 cgcagaaaat agtattttta atattgtaaa cacgcaaaca tgcacaaaac atttacacaa
     3301 cctcagtttg ttttatccca attttttaac ttccctaaaa caaattttgt ccattatttg
     3361 ctattagcca tattgctgtt aaatacaggc ttgcctatac atgcacaagc gccggaaatt
     3421 gaatgggcaa aaagttatgg tggcagtggt tctgaaattg cacataaagt tttgctgact
     3481 gctgatggtg gatattatgt ggttggatat accagctcaa attcgggaga tgttgcgttt
     3541 aataatgggt caatggatta ttggataatt aaactcaata gtgcaggtga tattctgtgg
     3601 gaaaatacct acggtggttc caattatgat tatgcattaa gtgcagtttt aactcccgac
     3661 gatggttgta tattagtcgg taaatcacaa tcgaatgatg gcgacgcaac cgataatcat
     3721 ggatttgatg attattttgc tgttcgtgtt gatagcgatg gcaataccat ttggaaaaag
     3781 agttatggtg gttccaattt tgaacaggct aaagcaattg ttgctaccaa cgatggtaat
     3841 tatgtaatgt atggttactc ctactctacc gatggcgata aatcaacaac ttatggcggt
     3901 gccgatattt ggttggtaaa aattaatccc gacggcgata tcatttggga aaaatcgtac
     3961 ggtgatgccg gtgaaaattt tggtgacgca attgtgcaaa gcagcacagg agattttgta
     4021 ttaacctggc atattgcagg tgccggcgac tatcgattaa tgaaattatc tgctgatggt
     4081 gatattattt ggaatcaaac atatggcgga actaacaatg atattgctta tgccattaaa
     4141 gaaaacacaa gcggcggatt tattattaat ggccaatcaa attctggtga tggtgatgtt
     4201 gtaggattac atggcgttgc aagtgatatg tgggcaatgg aaattaatgg cgatggagca
     4261 atcatctgga aaaaatgttt cggcggctct gcagatgaaa ttggcaatga tgtaataatt
     4321 aataattccg gaaattatgt tttagccggt atagcaacaa gcgataatga tggggatgtt
     4381 gttggtcatc atggtggtgc ttattcaggc gattattggg ttgtcgaatt agatgcaagt
     4441 cagaacatta tttggcaact ttctttgggt ggcacatcaa ccgatgaatt aaatacgatt
     4501 caacaaacac ctgataatgg ttatatcgcg gcaggctggt cagcttctgc gaatggtgat
     4561 gttaccgacc atatttttat tttcgatttc tgggtggtaa aactcagcaa taattgtgag
     4621 gaattaacgt ggtatgccga cgcggacaat gatggtttcg gcaatatttt aagtatgctg
     4681 actgcttgtg atatgccaag tggttatgtt gcaaatgctg atgattgtaa tgatgcgaac
     4741 gccggtattt ttccgggcaa tacagatatt tgtaatgctg tggatgacga ctgtgatgga
     4801 ttaactgatg aagatgtagt ttttacaact tattattttg ataatgatgc tgatggtttt
     4861 ggtgataccg acatggacac cataacatgc agtagtattt ttggatatgt attggacaat
     4921 accgattgca acgacacaaa cgcatctatt aatcccgatg caacagaaat ttgtaacgat
     4981 ttagacgata attgtaacgg cctcagcgac gaaggtttgc cggaatatgt attatttgct
     5041 gatgctgata gcgataccta tggcaatgca gatttgtata ttacatcctg ttatgcagca
     5101 attattggat atgttgttga tgcaacagat tgtgatgaca gtaacgcaga tattaatccc
     5161 gctgcagatg aaatttgtaa ttcgttagat gatgattgtg atggatatac tgatgaaaat
     5221 ttatccttta ttatttatta cgaagacgcc gattccgata cttatggcaa tgcagatata
     5281 gattctttag cctgtttaac accaactggt tatgtagcca acaacaccga ttgtgatgac
     5341 accaatgcag caatttatcc cggtgcaaca gaaatattta atggtattga tgataattgt
     5401 gatgaattaa ttgatgaagg cgtagctgtt catgagttgt cgcctgatgc aattatgcat
     5461 atcaataatc ctgtaaccac caatttacaa attaattaca gcggcaaaga aagtatcgat
     5521 ttttacatca tcaccatcac tggtgaaaat gtgtataaag gtcaactcat aacaggtgaa
     5581 aataatttgg acatgagtgc tttttcaaat ggattatact ttgtggtgac aaataatttg
     5641 aaggttgttc gttttgaaaa aataaattga aacaattaat tgtatttttt ggagcattaa
     5701 tattcccgct ccgaataata attcccaacc cgcttttcgc tgcaacgagc ttaacttctc
     5761 aattcttaga taaaaccggc tgttccattg gcacatcggt acatgattac attggtacat
     5821 tagctcgttt acgctgcaat cggggctagt tgaaggttca tatttttcat aaaaaatgtt
     5881 ttccgcaatc tggcagggcg gatgattatc cgcccttgca tatacttcaa attttgttca
     5941 atcaacaaat tttgctcaat cataaaataa tattccccct tgcatatgga tgccgggtaa
     6001 tgtaatagca cactttttct aattcgtttt acaattaaac ttatatgcta tctaaagatg
     6061 ttattcagtg gattcataat gaaaaacctt caaaggcata ctgactgcgc tgataaaggg
     6121 cttataaacc aagtattttc gggcaaatac aatgctaacg atttattaca ttcagttatt
     6181 tacaagatat tgttagtcaa atgaagctac ttaccattca gtttttctta aaatatccat
     6241 aatgatattt tcttcgtaaa atagaaatgg taaaaatcaa agtagtcttg cgacttaatc
     6301 ggtaattctc atgaaaacac aaagttgaaa ttagttgata tgtactttct ttgaatatct
     6361 tttttcgtcg gaaataatgc ttactgtgta tatacctgaa ggttgttcaa tattaaaact
     6421 agagttcaaa ttataaattg aattcgagaa gatttctttt ccggttatat cagcaacaga
     6481 tatttgtaaa tcattcgata gttcgctcac cttaacataa atcgtcttgt cgaaactata
     6541 gactacagca tccaatttat tttcatctgc gacatcaata taactgatcg tatcaccagc
     6601 aactatcgga tgatcgcact cagtttcgta tgcaaaatct tttatgacta gttcacgacc
     6661 aaaatctttt acatctaacc gaatccatcc ataatgataa caatttgccg tatccttaaa
     6721 atacactcct aaatatcgat ctgtcacctc tggataccaa tgtcctccat ggggccagta
     6781 agtactattt tcacccaaga atcgatacgc caattcttga tatcctgcat attgaaaatc
     6841 aagactttct tgatcaatga tagtgccttc cataagagca tatggatgat acacagttga
     6901 accaccatat gaagggaaaa tcacgtgcgg gtttcctgct attaaatttc caaagactag
     6961 aggcccaaca tatattgcgc taaaatgcga agtacttgat gaagtgggca taaaccagaa
     7021 ggatctcttt agaaatctga aatctgatag tccatcattg tttaaatcaa cagtatatgt
     7081 ttcccaatct tcattaatca atgtatccgg tataatatct ccgtaaaaaa cttgcgcttg
     7141 tacgtcattt ttaatgagta aaaagcttgc ggcaaacgca gaatattctt tgatatgaaa
     7201 ttttgatttt ttcaacctac tgtttaatga gttttccaac ggcaatattg ccatcttcaa
     7261 atttaatgat tataaaataa atcgcgtaac acaatctaaa tatttccatc agtggtcagc
     7321 ttgaaaccta gtggtgtatt aaatagagac ttggaattct catctcaacg ttagggtaat
     7381 tgaaaagtcc aatcttcaga agttaaagcc ctagtagttg cttgaatgat taattctcaa
     7441 aaggttcgtt taaaatcaac agttttcaac ggcgaaaata aataaggcca tttcacttta
     7501 tttaacttga attattatca aaatgtaatc agcaatactt tatggcatat gttcatccag
     7561 gcatgtatat gtttccaatt ttgttcaatc aacaaatctt gctcaatcaa aaaataaacc
     7621 ttcccatcaa aattggttga tacaaatccg ccatttatca aaatcccaat tttgttcaat
     7681 caacaaattt tgctcaatca taaaatctcc cctaatgcga aaaaccgccc taaagaagaa
     7741 cgaagttcac tccagggcgg tttttctgct ttagggtgga gttggtggga tttgaaccca
     7801 cgtccagaca aagcattagc aagctttcta catgcttaga tgtttcttgt ttgtcgggaa
     7861 gttcaaggtg aaacaccgaa cctgagaacc tccttatttc ctttatctca ctttactccg
     7921 gaaattagta aagccagccc gatgagatga cgttcaccga agttgtaccg gacaaccaac
     7981 atcgggaacg aaggctactt aattctctga attaagctgc catggcgtag ttagactcgc
     8041 cgtgtaaaaa ttgtttcgta gtttgagatt aaagcgccaa atctacaatg cgctgcatgc
     8101 ttacctgtca atgaacgatg ctgtcgattc ctgtcaaccc caataagtaa cagctaattt
     8161 cccggcaaaa cccttcagtt tcgccatttt acctgtcaca accaatgcaa aactacgccg
     8221 aaaagttcaa tttgatgcct aaacttatat atttgcacat aatggcagat caacaagata
     8281 cttcgcaaag cactaccagc ccttataaac ggataattcc ggacattgag gattggccaa
     8341 ttcatatcat tagcaaaaac cgtgagcagg tgattaaaag tgtagtggaa gaaacactgc
     8401 aaaacatcat caataaaaat gcaacggaaa aagacgtgca caatgaattg gctaaagcaa
     8461 tttatctcga aaaaattcgt gttgaacgcc cgtggaaaac agaccctcct gacgataaaa
     8521 aattctggac cgaagtaaaa aataaattgg tagaatttga aagtcacccg gacggaaata
     8581 ttgaagcaga aaaagaaatt ttacgccgaa taattacgcg ttacgtaacc gaaattgtcg
     8641 gcaattttaa taaaggcact tatcagtttt ctgaacgttt tgtgccattt gcatttaatc
     8701 gtttattaaa tgcttcgcat agtcgcgcat ttgtccgttt gttcggcaac aaaaaagatt
     8761 tactcaaaag tattcacgta attggtgaaa ttgaccaatt gcgcaacctt gctaaagatg
     8821 gtattgtggt tgttgtgcca acacatacca gtaatatcga ctccattacc gttggctggg
     8881 gaatatcggc aattggtttg ccggcttgtt tttacggcgc aggtctcaat ttattttcgg
     8941 tgcgtttctt agcgtatttt atgaatcgtt taggtgcata cagagttgac cgacgcaaaa
     9001 aaaatgctgt ttatctagag gcattaaaaa cgtatagcac ccaaacaatt tatcagggcg
     9061 gacatagttt attttttccg ggaggaacgc gttcgcgcag caatatggtg gaaacacaat
     9121 tgaaattagg tttgctgggt actgctcttg aagcacaacg tcgccaaatt attaatacca
     9181 atggtgaagg atttaaaaaa gtatatgtgg tgcccgttac cttaaattat ggttttgtat
     9241 tagaagcacc ggaattaatt gatgaacatt tacggaacgt tggtaaagaa agatattttg
     9301 tgccttccga taaatattct tcttcctata aaatattgaa atttttatat cgctttataa
     9361 ccgctgatgc cgatttcacc attgcttttg gcagatgtat ggatattttt ggtaaccctg
     9421 tcgacgacaa tggtaatagc ctggatcaaa atggtaatgt tatcgatatt cgcgattact
     9481 ttaaatctaa aggcgaatta aaatacgaca cccaaagaga tgccgtttat actaaaatgc
     9541 tcagtgaggg cattgtaaaa gcatttaagc gcaataatgt tgccctacct gaacatgtta
     9601 ttgcctacgc tgcatttaaa ttatttcagc aaaaacatca taccagcgat ttatattctg
     9661 ttttgcgtat gcctgaagaa ttccgcgaat tatcgaaaga tgaattgcgt gaagcggtgc
     9721 gcaaagtggt aaaagaactt accaagttgg agcacaaaga agaaattcgt gttgacccga
     9781 ttgtcaaggc agaagatacc gatgagctca tcgattatgc cgttaaaaaa ctcggtgttt
     9841 accatgcaaa acgaccatta ttaaaaacaa aaaaagggat gattaaatgt caggatatga
     9901 aactcctgtt attttatcac aaccgcttag aaggatacgg attagaaaaa catgtctaaa
     9961 aaatacagtg ttggtgtagt tggcgccggt agttttggta ctgccgttgc caatttattg
    10021 gctgaaaaca accatgtgta tttgtattcc cgcagagcag aaaatgtaga tgaaattatt
    10081 cgctcgaaaa cttcatctaa ccaaaaaatt cacgaaaata ttacgcctac caacgatatt
    10141 ggtttattga cccgcgaatg ttatttaata tttcccattg tttccagcga tggatttaaa
    10201 gatatgattc gcactatggc gccttttctt cgccctgacc atattttaat tcatggcaca
    10261 aaaggattag cggtaaagaa aaaggggttg gataaattaa aacaacgagc acgtattgca
    10321 ggaaaaaatg tgctcaccat gagtgaactc atccgctcag aaagtgtagt ggtgcgtgta
    10381 ggttgtatgg caggtcccaa tcttgccaaa gaaatttcag caggcttacc tgcagcaacc
    10441 gtaattgcca gtcgcttcga cgaagtaatt cacgaaggcc aaatggcgct taaaagtccg
    10501 aggtttcaag tttatggcag ctacgatatt gttggtgtgg aattagcagg tgtattaaaa
    10561 aacattctcg ctatcggcag cggtattgtg agcggattaa atttaggtga aaacgctcgt
    10621 gcattattaa tcacaaaagg ctgggccgaa atcatcaaat taagtgaagc gcttggcgca
    10681 gatgtaaaat cgtttttagg tttggcaggt atcggtgata ttattgccac ttgctcctct
    10741 cctaacagtc gcaactactc cgttggttac cgactggcaa aaggtgaaac cattaccgac
    10801 atcaaaaaca ccatggaaga agtagccgaa gggattaata ccattcgtat tgccaacgga
    10861 cttgccaatt attataaagt aaactgccca attataaaaa cgcttgcaca aggtatattc
    10921 gaagaattag atgtacaagc cggcatcgaa tatttaatga agtaccgtat gagtatggat
    10981 gtggaattta tctagaactc atctcaaaaa tacctttagc gtcttcgctc tttatccttc
    11041 gttgcttgaa ttgcccgtac ttcctccagt atgggctgca tcaatcgcct cgattaaaga
    11101 gcgaatactt gaaaatacta atacaaacct atttttgaaa tgagttctaa ataaaaaagt
    11161 tggaataaat atgacctcat tccaacttcc tgataatttc atttgaatta acttttccat
    11221 ttaatagtac aacctattgc ctttgtaaaa ttaacggcag gtttttcgcc tttttttaat
    11281 gcaccaatgg catcttcaac atattttgtt tttactgctg caggttcatc ggtattatca
    11341 tcaatggcac caatatattc aactgtaaat ttgccattgt tattttttaa tagatacaca
    11401 tgtggtgttc tggtggcacc aaaatctttt gctacctgct gagtggcatc aaataaatac
    11461 ggaaaactat acgccatttc ttcggctctt tttttcatgt tttccggtga atcttcaggt
    11521 tcacgaattg ggtcgtttgg gttgatggcc aaaacgggat atccttttga agcaaagttt
    11581 ttatccaact ccataatacg tgtttcatat gctttggcat agggacaatg attgcaggta
    11641 aatactacaa ttacaccttc acgagatgcg tagtcattta acgacacatt ttttccgtcg
    11701 atattggtca acgaaaagtt tttcacttca tcaccaaccg cataacctgt tgctgattgt
    11761 ggttggaggg aaagtaaggc tgcaatagcc gggattagaa ttgaaaacat atgttataat
    11821 tttaattaat gaaaatattt ctcgcatttt ttctcgcaaa ggcgctaagt taattgaatt
    11881 aacactgtgt gttatgttta aataaaaaac catttcaagc aagcaaaggt ggtattggtt
    11941 tatcagcttt gcatgcttga aaacatacca tatcgcacat cctatattcc tttttctccg
    12001 cgcctttgcg agaaccagac gtgtaaattt cacgcgagac gcggaggagg cggagatggg
    12061 aataaatatt gtttgatatt ttttaagaac gcaaagtttt tgttattgaa taaattcctt
    12121 tattttttga tcaagttgtt cgtaggtgat ttcagtttcg tgaataaaac tgataccacg
    12181 ttgtttgtta aacataattg ttgccggaat tgttccctgc cagcgtttat caatacggtc
    12241 gatccaatct tcaggcctgt ctttataaat tgcccacacc gatgaggcat aattatttgt
    12301 ttgtaaaaaa gggattaaac tactctccca acgcatctga gcatctaaag aaatcaaaat
    12361 tacttttacc ttttgatttt tatattcagc attaattttt tcaaaagaag gcaactcttc
    12421 tatacatggt ttgcaccagg tagcccaaaa attaacgaca tataaagtat cattggaagt
    12481 ttcaaaaaag ctgtccactt cagcaaatgt cttatactca actcgttgcg ctgcaacaac
    12541 ctgcatagca acaaataata tcagtgtaca cagccatttc atttagctaa aatacaatgt
    12601 atttccacta caatcaataa attaacacaa attaattccg agtgaaatca attttacaac
    12661 ctgcagtaat gccatattgc acacaaaaac cggcatttac ttccaccaca taaatggcag
    12721 ggccttttga tggaagtgat tgttcagaaa atggttctgc attttcagcg atggatacaa
    12781 tttctttttt agcattcaca taaataatat ctaaaggcag aatagtatta cgcatccaaa
    12841 atgcctgacg ttcttcttta tcgaaaataa acaacatgcc ctggtcatta ttcataaatg
    12901 aacggttcat taacccttgt tggcgctgac tatcatcatc ggcaatttcg atattgatat
    12961 gggtaacttt agttccatcc ggtttaaaaa agtcgagttc gccttctttg gtaaacttag
    13021 gctgaccatc ggtaagtgtg gccggttttt tgggttgtgg tttttcgtct ttgcaggagc
    13081 tcacaaataa aacaatagta aaggcaaaag ccagatggaa tatctttttc atagttactt
    13141 attaccacaa agatattgag aattgttggt tgcctgtagg cgcattcatc ttcaatttat
    13201 ccctattcac cgaaaaaata gccctaattg tggtacagcg catggtacac cgctttaagc
    13261 cccctacttt tggattaaaa ttatacaata tgacaatcat cgaagatcct aataagccaa
    13321 aaactccggt aactgaggaa agaaggccat tggaacatcg caggtattat ttaacaggtt
    13381 tggcattgct ggcggcaggt atattgtttt ttttggacag gtctaccgac tatgatttac
    13441 cattcccgct tatcagttgg gaatttatgc tcattatagc cggtatttat gtcggtgaaa
    13501 aaagtagctt taccggtttg agttggattg cctgtattgc tataggtggt tattttttac
    13561 tagacgattt tttcccgcat tttgatttac gattttattt cggcccattg tttttaattg
    13621 gtttggggat ttacttttta gcaggtccca gaactttccg ctaccgcaga tcacgtgcag
    13681 gtcgcaataa aagatatgaa aattaccgca ataccgttga aaaaggattc gcaagcgaaa
    13741 attcattgtc tgacgactat atagatattg tttccatttt tggtggtgtg caaaaacaag
    13801 ttactaccaa acaatttaaa ggcggcgaag ccgtgagtat ttttggtggt accgaaatta
    13861 atttaatgca agctgatttt gaagggaaag taatattgga acttactcaa atttttggag
    13921 gatgtacatt gattattcct ccgcattggg aattaaaatc tgaactcgtt tctgtatttg
    13981 gcggaagtga agataaccgt ccaagcacaa ctgtaacaga tgcaaataag gtggtaattt
    14041 taaaaggtac ttccatattc ggtggtattg agattaaaag ttattaagca gtaatatggc
    14101 aaccaacccc ttatttacca gtaaatatat taaggctgct tcgctgagtt tgtttatttg
    14161 gacggtgttg catgcaagtt ttatttattg gttgggattt gatattaaaa tagcattagc
    14221 agatgctgcg gcatttaatt ttttattagc tgatttttgt cttgcacaat tatttgtgat
    14281 gcaattttat cagcccaaaa aagaccaaac tgcatatatt attgtagcag gcactttaat
    14341 tgctacctct gttacttttc tcagtcgctt tttattaggg ttatttaatt tcgatgaaac
    14401 ttattataca tttatagata cctctattcc tgttcgtatt ggtgtagcat ttttgtttgt
    14461 agccattatt aatatgttta gcatgttagt gaaaggatta gaagaacaaa aaacaaatag
    14521 tgcacgcgcc tttgaagcag aaaaattatc taaagaagct gaattatata atttacgcag
    14581 tcagttacaa ccacattttt tatttaacag tttaaattcc atcagcgcac ttgccggctc
    14641 aaaaccggag caggcgcgga aaatggtgca acagttatct gattttttgc gcggcaccat
    14701 taaaaaagac aacctgcagc ttattccttt gcaggaagaa cttcatcatc tcgatttgta
    14761 tttagaaatt gaaaaagtgc gtttcggaca tcgcctggaa actgccattg tttgcgatga
    14821 aaattgtcaa acaaaaaaaa tcccttcttt attattacaa cccattttag aaaatgccat
    14881 taaatttggg ttatacgata cacttgaaaa tgtgcaaata gtaattagtg catcatgtga
    14941 tgaaaattat ttgagcatta gcatctccaa cccttacgac caaacttcat ctcccccaaa
    15001 aaaagggaca ggatttgggc tcagctctgt tcaacgcagg ttatatttat tattttcacg
    15061 caacgattta ttagatacca aacaaaccga caaccaattt attaccacag ttaaaattcc
    15121 acaacccaaa ttatgatgaa ggtaattatt atcgatgacg aaccattagc gcgcagtata
    15181 gttcgagagt atctggaaca acaccaggat atcgacattg ttgccgaatg tggtgatgga
    15241 tttgaagggg taaaagccat aactcaacac gaaccggacc tgatttttct cgacattcaa
    15301 atgcccaaaa taaatggatt tgaaatgctc gaattgctcg acaaacggcc atctgtcatc
    15361 tttactactg cctttgatga atatgctata aaagcatttg atattcatgc ggtggattat
    15421 ttacttaaac catttaataa agagcgcttc gacaaagcat tggaacgctg gagggcgatg
    15481 caaccgacaa cagataaaac tcctgaatta ttaaatacga ttgacgaaca ggaacaacat
    15541 ccgcaacgtg tggtgatcaa attgaataat aaaataaaaa ttattccttt tgctgatatc
    15601 atttatattg aagctgctga agattatgta tttatacata cttccgaagg tcgttttatg
    15661 aaacacaaaa ccatgacctt ttttgaacac gcgttagaca aaaatatttt tgtgcgttgt
    15721 caccgcagtt ttattgtaaa cgtacaatta attaacaggt tggaagtata tgaaaaagat
    15781 tcgcatgttg cgatacttac taccggtgca aaagtgcagg tgagtaaaag tgggtatcag
    15841 cgattaaaag ctgttttagg ggtataacat aaaaaccgga atgttatgtt ccggttttta
    15901 tctgctattg tgatgattta gcatttttct tggctatttt ttcagccttc ttttcctttt
    15961 tagttttttc aggtttcttt ttagtttctt ttactgcatt ttttcctttt gccatggtag
    16021 ttacatttta ttatgcaatt ataataaatt ttataccatt tgattatcct gttcgaaaaa
    16081 tgttaagcac attggatgca ccatatcagt tatgagtgac caccaattga aacactttat
    16141 aattgtaacc attattaata ttcaccaaat acaggccatt cggcaggccg gaaacatcaa
    16201 tagaacagga ggtttggccc tttggtatta cggtttcgta aactagttga ccactgctgt
    16261 ttaccacttt taaattggtt gtaaataagc cggtttgcaa attattgatt tcaacaaaac
    16321 ttgcagcagg attgggatac atacgacaat tgtcaaagga atttgtttct tctaaactta
    16381 ttgtcgaggt gtcttgagtt gcagtatcag cacaaatagt agtaaaacca gcgccgctaa
    16441 accaaattgc tccggtatca atggaaaagg taatgggcat cgcaaaacca ccaaggttat
    16501 atacgccatc atacacttca accgtaccgc ctgaatcggt tttgctgcaa ttataccctt
    16561 tatcgtcgaa ttgaagcaca tatatttggt cgtataattc tccgttagga ataccgtgac
    16621 cggtaagtaa aaaatggttg gattttgttt ccaataatcc gctggctgct tctatttcat
    16681 cctgaccagc aaaggaggaa aatacaattt gcatagcggt atccagtttg agcagataat
    16741 aatcatcttc gcctgaattt tttgaaccgg caatggcata ttgattatca tgtgtttgaa
    16801 ttatttgcgc cgtcatttcc gaagtgcccg aattaattat tttttgccat tgcgtattac
    16861 catcaccatc tatctcactt aaaaaaatat cataattatc ttcaaacgat gaaccagcaa
    16921 tgataaactt gtcgctatta acagcaacaa tactgtttcc cgattcaaat ttatcggctt
    16981 ttcctgttct gttaaaccag cttaaagcgc ctgcagaggt gaattttgcg acaaacatat
    17041 cgccagttgt caccgggaaa cccgtatgta agtaaccagt tacaattaaa ttgccatcct
    17101 gtaattggac tacatccatt gcttcaccgc tgcaagaacc acaaacggta aaacgccggc
    17161 tccattccac ttcaagcgct gcatttaatt ttaatatcat aggctgatag gtaattaagg
    17221 ccgcaccaac agtatcggta taaccaacaa taacataacc gccatcaatt gttttaatca
    17281 gctgattccc gatattactt gtttcatcac caataaattt cgaagtaatt aattcacctt
    17341 cagcatttaa ttgaaccaat gcaacatcat aaccaccaac ctcggtatat gcactacctg
    17401 caattaaaaa agatccatca tttaattcaa ttgctgcttt tgcaatatct tccatttcgg
    17461 tgtatacaac agtgctccaa atttggttag cagcagagtc tgttttaaca atataaaaat
    17521 caaaaaaatc gcctgaccag gaattagtat ttccggtaat taaaaaccca ccatcggaag
    17581 tctggatact ttttccggaa tattccgttc caacatcacc atacgaacgg caataggttt
    17641 gagcaaacat ttgttgcact aaaaccgata ctagcaggag gagtacaaag cgcatatcgt
    17701 ttcatagttt aatgaactac taatatacaa aaaaatccct gttaccgcag caacagggac
    17761 tttatatttc acatctttct gtttcggatt aacgcatatg attacatcat accgtccatt
    17821 ccgcccggca ttccgccacc cataggcata tcttttttct caggtttgtc tacgatagtg
    17881 cattcggttg ttaataataa tccggcaata gatgcagcat tttctaatgc tacgcgggtt
    17941 actttaacag ggtcgattac accggcttta agcaggtttt catatttttc agttctggcg
    18001 ttaaatccga aatcggcttt accttcacgc actttttgca caacaattga accttcaata
    18061 ccggcattgg caacgatagt tctgattggc gcttctaaag cacgtttaat aatgtcaact
    18121 ccgatttttt catcttcatt gcttactttc aatttttcca atacttcgat agcacggata
    18181 taagcaacac caccaccggc aacaatacct tcagctactg cagcacgagt tgcatgtaaa
    18241 gcatcatcca cacggtcttt cttttctttc atagccactt cagtagcagc acctatatac
    18301 aatacggcaa caccaccact taatttagct aaacgctctt gtaatttctc acgatcgtaa
    18361 tctgaagttg ttgtttcaat ttgcgattta atggtgttta cacgaatttt aatatcgtct
    18421 tttttacctt ttccaccaac gatcgttgta ttgtctttgt ctacagtaac tttctccgca
    18481 cgaccaaggt cacctaattc aacactttcc aacattttac cttgttcttc gctaatcata
    18541 gtaccaccgg ttaaaatggc aatatcctgc aacatttctt ttcttctgtc accaaaaccc
    18601 ggcgctttta cagcacatac tttaatggtg cctcttaatt tgtttactac taaagtagtt
    18661 aaagcttcac catcaacatc ttcagcaata attaataatt gagaaccttg ctgagcaact
    18721 ttttctaaaa ttggtaataa gtccttcata gtgctgattt ttttatcata aatcagaata
    18781 taaggacgat ccatttcagc ttccatatca tcaccattgg tgataaaata tgctgataaa
    18841 taacctctgt caaattgcat accttcaact acatcgctgt aagtatcagt acctttagct
    18901 tcttcaacag tgataacacc gtctttagaa actttttgca tggcagtagc aattaatttt
    18961 ccaatttcag catcattgtt tgcagaaatc gcagctacct gttcaatttt tttattgtcg
    19021 ttaccaactt gttgagcttg tttttttaat tcgattacca cagcttcaac agctttgtcg
    19081 ataccacgtt ttaaatccat aggatttgca ccggcagcca cattttttaa accagcagtc
    19141 acaatagcct gagctaacac ggttgcagtt gtagtaccat caccggcaac atcgctggtt
    19201 ttgctagcta cttctttcac catttgtgca cccatgtttt cgattgggtc ttctaattca
    19261 atttctttcg caactgaaac accatcttta gtaacatttg gagcaccata ttttttttca
    19321 attaccacat tacgaccttt tggtccgagg gtaactttta ctgcattcgc taatgcatca
    19381 acacccgctt ttaatttttc gcgagcttca acatcaaatt ttataatttt tgccatagtt
    19441 atatttttaa atatttattt ctaccgtaat aaaatgtttt tgagattata aaatcgcgaa
    19501 gatatctgcc tcacgcataa tcaaatattc ttgtccgtct acagtgattt cagttccggc
    19561 atatttgcca tacaatactg agtcaccaac tttaacagta accggctcat cttttttacc
    19621 gggaccggct gctacaacaa tacctttttg cggtttttct ttcgctgtat caggaataat
    19681 gataccaccc tttgtaacag tttca
//
LOCUS       DAORKW010000014        19698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_23_length_19698_cov_5.253882, whole genome shotgun sequence.
ACCESSION   DAORKW010000014 DAORKW010000000
VERSION     DAORKW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19698
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23_length_19698_cov_5.253882"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLB46_01355"
     CDS             complement(<1..446)
                     /locus_tag="PLB46_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75185.1"
                     /translation="MSDFTLNDESWKVYNEPAGGAPVLPINWSATGGNPGGYISETDI
                     SIGAWGWIAPSEFKGDLSAYYGCNLSYDLYQNTFAGDMSNYYDVMIFNTTGDTMVYNY
                     PDLNVIYTWVNKVVPLTDVAGWEYGESIGGGAPAATPAQILAILRD"
     gene            complement(718..1467)
                     /locus_tag="PLB46_01360"
     CDS             complement(718..1467)
                     /locus_tag="PLB46_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HRF75186.1"
                     /translation="MTKISLAAVSTLPPNTYDKGDTEEATEKLIDELRELQNVLFAQQ
                     KFSLLVVLQGMDASGKDGVIKEVFSGVNPMGCHVQGFKKPTEEEMGHDFLWRIHRNMP
                     AKGMIQIFNRSHYEDVVIQKVHKWVDDDTIQRRYEHINAFEKLIQENGTVILKFYLHV
                     SRKEQLERLQERLSDPRKMWKYNANDIEESKRWDEYMEAYEAAFNKCSPEIPWTIVPS
                     DKNWYKEYLIAKKIVEALKALDLKYPELPTK"
     gene            1589..4234
                     /locus_tag="PLB46_01365"
     CDS             1589..4234
                     /locus_tag="PLB46_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF75187.1"
                     /translation="MAKKNEEKKRFKFKEMKVYGSLENFFGNQKNYRIVFDESEVCYV
                     NVELQLFNILFDETEWKAKVKIKASEYYNNKEMCNIDKEIVVTTDQNIIMIREGWGTP
                     NPGWWKKGTYKWEVFLDDVIVGTNYFYITDNGMVTSANNPYFDINAIKLFNSPKGTLP
                     REQRKYLTTFNKDTAQYISIELDITNKISHEKYFPLELNFYFYNDSRQLKGACYWFEH
                     VNDSRKNILMDVGYGAEGTGFWYPDKYTCEVVFMDNIIAIVPFEVGTTDIELSGTPVW
                     LTSITQGDEVHAIAAEQQDEATYEAAKKELDDLIGLQTVKNEINELTTYLKFLKIRQE
                     KGFEEKNKINLNMVFTGNPGTGKTTVARMLGKIYKSLGLLKRPDVTEVGRVDLVAEYI
                     GQTAPKTKAAIDRARGGILFIDEAYALSDRGDDGKDFGKEVIEVIIKEMSDGPGDVAF
                     IFAGYPKEMRDFLNSNPGLSSRVSQVINFPDYNPDELMDIAAYDSNKRGVLISEEARN
                     LVYMKVVEAYRNRDNKFGNARYVNSIIEEAKQNMALRLMKSAKPEDLTKEDLSTIILS
                     DVEEIFGLGSAKKVDLPIDEVLLQDAMTQLKELTGMANVKNEIEETVKLVRYYKEIGR
                     DVKKSFSLHTVFTGNPGTGKTTVARILVKIFKALGILERGHLVETDREGMVAGYVGQT
                     AIKADSMIEAAMGGGLFIDEAYALSQGGENDFGREAIETLLKRMEDHRGEFIVIVAGY
                     TDNMAKFLESNPGLKSRFDKFIHFEDYQSDELFVITENLLNKEFLFLNEESATHLKTF
                     ITQLVSNRNKYFGNARTMRKLVADIVHHQNLRMAGIPAAQRTPEMIKTVTIDDVKSFS
                     IDIEGKTTVRESIGFKLGTGNKNPN"
     gene            4535..7441
                     /locus_tag="PLB46_01370"
     CDS             4535..7441
                     /locus_tag="PLB46_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75188.1"
                     /translation="MKKILFTISLVTSFIMADGQWTNTYNGQGDFSDVFNSIKVDASG
                     NTYQSGYTINPELSKDILLIKLNNAGDTSWTRIFNGPGNGPDEAIAMTLDIDNNIILT
                     GYQKGSGTGYDIITLKYAGNGELLWQVTYNYTSNEFDQSNAVITDADGNIYIAGQSDK
                     DETLNNNDDFLIIKYNSSGIQQWAKRTNGLGNSNDRPSAITLNTENDVVVTGRSFNGN
                     DDDYFTVKYDGSTGTELWKKYFDRTHNDRATAIVCNTANNRIYITGRSTNGDNYDYAT
                     VCYDNAGVEIWQSIFDYVDDDRATHIGLDNSGNIYVTGQSDVNASAIGVNYDILTVKY
                     NSLGVQQFAQTYGGTATGDDIPSGLQFDNSGNIIICGSTDMDASALIKNDFVVLKYNT
                     AGTLQWSTTFDGSGTNDNAKSLAVDGTGNIYVAGYSEAVPNKDAATIKLNNAGALQWD
                     YNFNGIGDNADNVHAIKKDNSNNIYLAGYTFGYEQDRNFLLMKIGASGTMQWQHVING
                     SSNTQSTDDAITLQLDNAGNIYAAGFVKNSGTGYDMMIVKYSTSGDSLWSYNYNYTLA
                     NETDKAIALELDADNNIYVVGRSDQDPTIVSNDDIVTLKLSSTGNLLWEKRYNGAGNS
                     NDNVKALFVTGGGDIYVTGKTTNGGQSDIILIKYNAAGVQQWVKTFDRSNGDDEAIAL
                     WIDASDNIYVGAETTSANHDLDVTILCYSNNGTLNWQKHYDSGLHGNDETKSLHADNE
                     GNIIIAATTAPDTALLTLNGDITILKFDNTGELLWEQTFENELNDDASEATIDANNNI
                     IITGQTDNGTTGYTNYDYLTLQYLPDGTLYTTELYNGPDNLSDVPNTLIAFENAFYVT
                     GGSYGNSSQRDIVTILYGTTPEVVENIQDNPLQFTVFPNPATTQFQINTPNNESGLDL
                     SVRFQLFDIQGRLLIEIPETTSPTINCSQLPRGIYVLNAVMNGEILSTQKIILN"
     gene            7469..8515
                     /locus_tag="PLB46_01375"
     CDS             7469..8515
                     /locus_tag="PLB46_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75189.1"
                     /translation="MNNRIKKTIICKEVTNYTVNHAINNSYTPKDGDVALFEVLVIGK
                     HTSVQSETKRITAIFEGDVIMAAFANRYATSQFEGYTPDTPTEVLDILGAGGAIGLVK
                     SKNASLEDIEPTKVRLIGYATGVDGNVINTKFYHTNRIPFTGFVPNNAKLILSVGSSM
                     DSGKTTSAAYLARGLKTTGKKVAFIKLTGTSFTKDKDFVFDCGADVVVDFTDGGYPST
                     YMCSKEEIMDLYQTLLYMLEPENPDYIVMEIADGLLERETDFLLRDTAFMSTVHSMIF
                     SCGDSLGVFYGLQLLEQFGVTPAVICGRFTMSPLLIQEVKAKIDIPTLDIDQLSNGEY
                     VHLFDQQKVFVHHA"
     gene            8508..10181
                     /locus_tag="PLB46_01380"
     CDS             8508..10181
                     /locus_tag="PLB46_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF75190.1"
                     /translation="MLNKFDVIRSFAVKKRGIITGAFFTGVISTGCSVIIPLFIGQYY
                     NIVFHSQSSRGAIFEKLFGHIENVTFFLIVFGLFLLFRLVFTFLEKYFIGLTGESFAN
                     TIRQQLFSKQLHTELNTFESKDTGVYLLRYSGDLKSVQDYLTKGIIQFCYDCFFMLGA
                     FILLYILNAQLAFIFVLAIPVLLITNAILNKRLKAITGQRRNLKSCNLAFVATRLKSV
                     LTIKLFNRESIEADKYNKRSTESFKIGKKYQAIAAFMQALYPFILYSSLLAILWVAYL
                     QVNSDTKTIDGSTLVTFIMLVISIIPVFKRLLKVNMIWVSGNVSFSKLLLLFNANEEN
                     KAKTPKINRIKGSLKAEDISFKYSNDFVLQNINFTIEPGTITALVGPQGSGKSTLFKL
                     ITGLYPLDKGKLFLDNYLVADLSKHILRKNITIVSDNLPLLGRTYFEAISYSRQEKNR
                     AAGKALLSELGILGGSENDVLNKQIIDGGRNISNGEKKLLNIARALLTNKKIILMDEP
                     FSDLSPKAIELVCKCIEKRRSKHTIIIADNLNINIAYDNRITLHEKISN"
     gene            10162..10578
                     /locus_tag="PLB46_01385"
     CDS             10162..10578
                     /locus_tag="PLB46_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75191.1"
                     /translation="MKKSVIKIILIIVFATNLWSCTKEPGIGGEATISGKVYAKHYNS
                     TFTVLLSEYYVPDTYVYLVFGDDVNYGTRLKTNYDGEFEFNFLYEGEYTIYTYSIDSA
                     AIVNFAVDPPETAIIEKVNINSKDANIDIGDLIIYK"
     gene            10585..11148
                     /locus_tag="PLB46_01390"
     CDS             10585..11148
                     /locus_tag="PLB46_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75192.1"
                     /translation="MKYLLILFTILSIGVKGYSQSGTARGARALYNEVSELKNGQLQI
                     VKRTYQEFDKKGRVTLEVAFGLDSLMKSKETFTYDKKGNETIHTIYNSANQIESKILT
                     TYNRFKEKKNITVYSGTDSLLEKTTFTYDNFGRKSLELSVDANNKQIKKITFSYDQRG
                     ALNKRVIYDGADNITQIKQYNYTYSTK"
     gene            11145..11810
                     /locus_tag="PLB46_01395"
     CDS             11145..11810
                     /locus_tag="PLB46_01395"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRF75193.1"
                     /translation="MKLYSLLILFLFWPLLQFAQQQDAIGSFAFQLEKKLNQTFSVSY
                     YSQVGFNENITEYGYSLYDLGLNIKLTDNFYAGYNYRIANIKSNENKYFDRQMMYVDI
                     GWVKSFGKFNLNIRTRYLNKWYGWNFDENNNYKDNAHYFRNKIQLKYAINYNYSIYAG
                     CEQIYRLDLRNETEQLRYGGGINYQINSKNKLQLSYIISREQNRAKPDTNFTTGLTYY
                     FKF"
     gene            11820..12890
                     /locus_tag="PLB46_01400"
     CDS             11820..12890
                     /locus_tag="PLB46_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRF75194.1"
                     /translation="MKSLSIAAFVGLFSLLFIACKEQVETSQPIQQKAAFALAIHGGA
                     GAMPKGSYTPEQEAAFHAKLKEAAQLGYEMLSRGDSAVAVVEAVICVLEDSPLFNAGR
                     GSVFTNNGKIKMDASIMNGRNLDAGSISNVQRIKNPIKTARMVMDSSKYIMFSSAGAE
                     RFAEKYKLELVDVSYFYTQHQYERWKGMKDSTEGNYIKYVDSVMALQEEPVALTNIIE
                     KYGTVGCAVLDKYGNLAAGTSTGGLMNKKYNRIGDSPVIGAGTYANNKTCAISCTGTG
                     EDFIKTVAAKTVSDLMEFKGFSLEEATNDMIHVRFKEITGDGGMIAVDNLGNISFQYN
                     TDGMFRAKVDEKGNISTYIYNE"
     gene            12883..13779
                     /locus_tag="PLB46_01405"
     CDS             12883..13779
                     /locus_tag="PLB46_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75195.1"
                     /translation="MNKFLLIAGLLLLFACNNEKTQTPVAHSKKFTYQLSQATKICRQ
                     EDWFDESGKIIKTIHYGNRENDTLNITNYYYSGDLLVEQLRIKPDGKIMDKTVYHYTL
                     GSLKGESFIIGADTLTTKYFTYYDNGSLKRTTTVYNMEKNNPVTTVTYLDEHGNVEKI
                     YNQIYEDSTKIVLSRYEMQSYKNTYNSLGKLESSIGTMLFGYYETTDTISITSFRYDA
                     SGNMTQQLNLKKTNSGEPDSIVYQYNASNILTQKIAYITAKQKNEVVSIIDTTSYIYD
                     TNSRLIKEFSSHYNKGFEYKFE"
     gene            complement(13912..16041)
                     /locus_tag="PLB46_01410"
     CDS             complement(13912..16041)
                     /locus_tag="PLB46_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF75196.1"
                     /translation="MLNKYLIVGFNIIFSVSVVIGQAPIEKDWDYRFGGTEYDNLDGV
                     LELPSGEIFLYGTSSSDAEGDKSQDAYETWPGINSGDVWVLKLDAFGEKIWDYRYGGT
                     NADAAYDCVLLADGSIMLAGISSSPVSGEKSQPSWGYYDYWMIKIDADGNYLWDKRFG
                     TTASENLTCIVQTQDGGFVLGGYTEGDNNGDKTQPAWEDGSYEYDYWIVKTDAVGNKL
                     WDKRYGGLEADLMHGIVELPDGSLMLGGYSYSGVSGDKTTNQEGLGDYWVVKTDAEGN
                     YIWDKKYGGNLEEKVYHMILDNDNNIIIGGNSRSDISGDKTEPAYLHPWYGNSTDYWI
                     VKIDLDGNKIWDHRYGGEDFEDEFGNIFQTSDNGYLLSGNSYSWPSGEKTEENLGEEQ
                     MWSLLIDNNGTVLWDKTIFTDSHEELGLGIQLQDGCYLFATYSYAPVSGYKTQSPYAF
                     LKDDYWITKFCDTTAILPIANFTNELDTICQTSCLNFNYTGSGASSFLWEFEGGTPNV
                     ATTANPVICYENAGNFNVTLIVTNDAGADTITMENIITVLPSPAEINLPETAILCNYE
                     SLVLTVGSETATWSTGETGSEITIFEPGLFSVTDSGYCGAVIDSVLVIAQQCNCELLK
                     PNAFSPNNDGANDVFRLIAACDIDFTYLHIYNRWGEKVFETTNIETAWNGTFKNENQP
                     MGTYVYVVSYINENGNQVEEGNITLLR"
     gene            complement(16052..19201)
                     /locus_tag="PLB46_01415"
     CDS             complement(16052..19201)
                     /locus_tag="PLB46_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75197.1"
                     /translation="MRKISLPNLVAIMIVMMFLILSNTRAIGQTTLWKNRLNGSGDNA
                     DRYNGAVKDAAGNFYLTGYTVSPGTGKDFLTVKLNSAGDTVWTRKYNYVANFDDEANF
                     IVIDALGNITVSGYSDGGSTSTKNDILTIQYNAAGALLWATRYNNAAVNEDEFPASLC
                     VDAANNIFISGRSDHDAGNIDDVITIKYNNLGVQQWAAQYDGGLTDRAAGIVADNAGG
                     CYITGRTDTGGSEDILTISYSSTGTVNWTVLYNGPGGDDRGQAIALDATGNIYVGGIR
                     ANASDDDFVTLKYNTAGVLQWSKIVNGGDNDRLTTIKTDALNNVYITGQTDIDAGGNS
                     DYNFRTIKYNSAGVLQWSAITGNPANQEDVPSDLLVDAAGNVYVTGKSDAIAGGAVNF
                     EFMTVKYNSAGAQQWLKYYDGTVANAEDIPSTIIVDAASNVWIAGGVSFTATQKDATF
                     IKYNSTGVFQIAKTYNGKGDFTDKAKAIATDASNNLFVTGYTLVEAQQRNVLVQKINS
                     AGTTLWSRNVNGTNENDEGLAVTTDAAGNVYVAGYSNGSGTYDDGLILKYNTSGVLQL
                     NIIYNYVASQLDKFVSIAVNPAGDIFVTGYSDSNVDPLITNYDWITLKYNALGILQWA
                     TRYNGVGNGDDKAAKLVLSGDKIFVTGYAFNGTNNDIVTIKYDNAGTQLAIATRAGSG
                     GDDEGEDMFISGASVYVTGTEFVTGNLGDYVTIKYDTLLNQLWLKTYNGTADAEDRAY
                     GVSATATDVYVTGKSAGVGSANDIALIRYNASTGAQNWAKRYTGAGALNDEGYNVVTD
                     GGGNIYTGGVTGNAATVADFVALNYNTAGTKLLTLKYNGTGNNEDVGKQVILDNNGYL
                     YITGYSVGTGTANYDFSTVKFCTPLPEATITAGGATSICIGDSVLLSANIGAGLTYQW
                     KKGAANIAGATSSNYNAKGAGAYKVVVTNSNGCTKTSNTISVTIIICKDGEVINNQME
                     YTISPNPFGTATQLQLANVNENVQLAIYDITGKLVYSTNIEAGTESIELGQQLLPGAY
                     MMVLENAGSVQTYQLIKAE"
     gene            complement(19503..>19698)
                     /locus_tag="PLB46_01420"
     CDS             complement(19503..>19698)
                     /locus_tag="PLB46_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRF75198.1"
                     /translation="FDNEAKAGDQEFEDRGEKLVTDLKSFLYLCDTTLDYSDGLNGKG
                     FQFVNPNASRTCGCGESFAV"
BASE COUNT         6582 a         3535 c         3487 g         6094 t
ORIGIN      
        1 aaatctctca aaatagctaa aatttgtgct ggggttgcgg cgggagctcc accgccaatt
       61 gattcaccat attcccatcc tgccacatct gtaagcggaa caaccttatt tacccatgta
      121 taaataacat tcaggtcggg ataattatat accatggtat caccggtggt attaaaaatc
      181 atgacatcat agtaattact catatcacct gcaaacgtgt tttgatataa atcgtaagat
      241 aggttacaac cataatatgc agataaatca cctttaaact ccgacggagc tatccaaccc
      301 caagcgccta tggaaatatc agtttcagaa atatatccac ccggattacc acctgtagca
      361 gaccaattta taggaagcac aggcgcccct ccggcaggtt cattatatac tttccaggat
      421 tcgtcattta aagtgaaatc gctcacaatt tgagcataca aggcctttcc actgcaacag
      481 atgcaacaga aaaacatcaa tatatgtgtg tatttcgaaa ataaacgcac ggaaaaaaga
      541 cttttagcct taaaatacac tacaagatta ctaagctgca aatcaaattc caaaactttt
      601 acacaaaatt tttagtgatg caaccttctg aaaaccaata acgtcggggg aaaagtagca
      661 aactaagcat catttttatc cagatgttaa caaacggtat tgtacaatca tcccaattta
      721 tttagtcggc agctcaggat acttcaaatc cagtgctttc aaggcttcca caattttttt
      781 tgcgatgaga tattccttgt accagttttt gtcagatgga acaatagtcc agggaatttc
      841 ggggctgcat ttgttaaaag ccgcttcgta agcttccatg tattcgtccc agcgtttact
      901 ctcttctata tcgttagcat tgtatttcca catttttcgg gggtcagaaa ggcgttcttg
      961 aaggcgttca agctgttctt tccggctgac atgtaaatag aattttagta ttacggtgcc
     1021 attttcctga ataagttttt caaatgcatt gatatgttcg tatctgcgct gaatggtatc
     1081 atcatcaacc catttatgga ctttttggat tacaacatcc tcataatgag agcggttgaa
     1141 aatctgaatc attccttttg ccggcatatt acgatgtata cgccataaaa aatcgtggcc
     1201 catttcttct tctgttggct ttttaaatcc ctgaacatga caacccattg gatttacccc
     1261 tgaaaaaact tctttaataa caccatcctt gccactggca tccattccct gcaaaaccac
     1321 caaaaggcta aatttttgtt gtgcaaataa cacattttgt aattcacgca gttcgtcaat
     1381 cagtttttcg gttgcctctt cggtatcacc tttatcgtag gtgtttggcg gaagggttga
     1441 aacagcagca agtgaaattt ttgtcatagt caggtatttc tccctgcaaa tgtagtgaaa
     1501 ccaatctcat aaggaagccg gttcaatcgt ttgacaattc gtagaaatat ctaaatttac
     1561 atcctcccta aacgaggata tttgtattat ggcaaagaag aatgaagaaa aaaaacgctt
     1621 taagtttaaa gaaatgaaag tgtatggttc gcttgaaaat tttttcggca accaaaaaaa
     1681 ttatcgcatt gtattcgacg aatcggaagt ttgttacgtg aatgttgagt tgcaactatt
     1741 taatatttta tttgatgaaa ccgaatggaa agctaaagta aaaattaaag caagtgaata
     1801 ttacaacaat aaagaaatgt gcaatatcga taaggaaatt gttgtaacaa ccgaccaaaa
     1861 tattattatg attcgcgaag gatggggaac tcccaatccg ggctggtgga aaaaaggcac
     1921 ttataaatgg gaagtatttt tggatgatgt aattgttggt accaattatt tttatattac
     1981 cgataatggt atggtaactt cagcaaataa tccctacttt gacatcaatg ccattaaatt
     2041 atttaatagc cctaaaggca cacttcctcg cgaacagaga aaatatttaa ctacatttaa
     2101 taaagatacc gcacaatata tttctattga gttagatatt accaataaaa taagtcatga
     2161 gaaatatttt ccattagaat taaattttta tttctataat gattcacgcc aattaaaagg
     2221 agcttgttac tggtttgaac acgtgaacga cagcagaaaa aacatattaa tggatgttgg
     2281 ttacggtgca gaaggaaccg gtttctggta tcctgataaa tacacttgtg aagtcgtatt
     2341 tatggataat attattgcta ttgttccatt tgaagttggc acaactgaca ttgaactttc
     2401 aggcactccc gtgtggttaa ccagcattac acaaggtgac gaagtgcatg ctatagcagc
     2461 cgaacaacaa gatgaagcaa cttacgaagc ggctaaaaaa gagttagacg atttaatcgg
     2521 attacaaaca gtaaaaaatg aaattaacga actcactact tatctgaagt ttttaaaaat
     2581 aagacaagaa aaagggttcg aagaaaaaaa taaaataaat ttaaacatgg tatttaccgg
     2641 caatccggga acaggaaaaa caactgttgc aaggatgttg ggaaaaatat ataaatcgct
     2701 gggattacta aaacgacctg atgttacaga agttggtcgt gtagatttag ttgccgaata
     2761 tatcgggcaa acagcgccaa aaactaaagc ggcaattgat cgcgctcgcg gtggtatttt
     2821 atttattgat gaagcttatg cgctgagcga tcgtggcgat gacggaaaag attttggaaa
     2881 agaagtaatt gaggtaatta ttaaagaaat gagtgacgga cccggagatg tggcatttat
     2941 ttttgcgggt tatcctaaag aaatgcgcga ttttttaaat tccaatccgg gattaagttc
     3001 acgtgtttcg caggtaatta atttccctga ttataatcct gatgaactga tggatattgc
     3061 cgcttatgat agtaataaac gaggggtatt aatcagtgaa gaggctcgta atcttgttta
     3121 catgaaagtg gttgaagctt atcggaaccg cgacaataaa tttggcaatg cacgttatgt
     3181 aaattccatt attgaagagg caaaacaaaa tatggctttg cgtttaatga agtcggcaaa
     3241 accggaagat ttaacaaaag aagatttgag tacaattata ttaagtgatg ttgaggaaat
     3301 tttcggatta ggttctgcca aaaaagtaga tttaccgatt gatgaagtgt tattgcaaga
     3361 tgccatgaca caattaaaag agctcaccgg aatggcgaat gtaaaaaatg agattgagga
     3421 aacagtgaag ctggtgcgtt attataagga gattggtcgc gatgtaaaaa aatcattttc
     3481 attacataca gtatttaccg gaaatccggg aacaggtaaa acaactgttg cacgaattct
     3541 ggtgaaaata tttaaggccc ttggaatttt agaacgcggt catttagttg aaactgaccg
     3601 cgaaggtatg gttgcgggtt atgttggtca aaccgctatt aaagctgata gcatgattga
     3661 agcagcaatg ggtggtggtt tatttattga tgaagcatat gccttaagcc agggtggaga
     3721 gaatgatttt ggtcgcgaag caattgaaac attactaaaa aggatggaag atcaccgtgg
     3781 tgaatttatc gttattgttg ccgggtatac tgacaatatg gcgaaattcc tggaatcaaa
     3841 cccgggatta aagtctcgtt tcgataagtt tatccatttt gaagattacc aaagcgatga
     3901 attatttgtg attacagaaa atcttttgaa taaggaattc ctgttcctga acgaagaatc
     3961 ggcaacacac cttaaaacat ttatcacaca attggtaagt aatagaaata agtacttcgg
     4021 aaatgcgcgc actatgcgta agctggtggc agatatagtg catcaccaaa acctgcgtat
     4081 ggccggcatt cctgcagcgc aacgcacacc tgaaatgatt aaaacagtca ccattgatga
     4141 tgtgaaaagt ttcagcatcg acattgaagg aaaaaccacg gttagagaaa gcattggctt
     4201 caaactaggc acaggaaata agaatcctaa ttaatctagt aaacgtatca taaatcgcca
     4261 ttccgcaagg tttggcgatt tttttttgcc tttttttcac ctttttcaaa gtaaaatagt
     4321 taccaatttg gtgaaaactg cctttttctg tccccatctg atggccaaca tttccgcaca
     4381 aaacaagggg tttaagaggg aaagtatctg tttatacata tattttttat gaaagttgcc
     4441 cataactgaa cggaaaacaa aggtttttgt cctttttgta tgtagataca cgcaatactt
     4501 ttgaaactgc aaacgaactc ttaaccgcaa aacgatgaaa aaaatattat ttacaatcag
     4561 ccttgttaca agttttatta tggctgatgg acaatggaca aacacctaca atgggcaggg
     4621 tgattttagt gatgtgttta attccattaa agttgatgct tcaggcaata cctatcaatc
     4681 tggttatact ataaatcccg agttaagcaa agacatatta ttaatcaaat taaataacgc
     4741 gggtgatact tcttggacac gtatttttaa tggtccgggt aatggtcctg atgaagcaat
     4801 tgctatgacg ctggacatag acaacaatat catattaacc ggatatcaaa aaggcagtgg
     4861 aacaggttac gatattatta ctttaaaata tgcaggaaat ggtgaattat tatggcaggt
     4921 aacttacaat tatacatcaa atgaatttga tcagagcaat gctgtaataa ctgacgcaga
     4981 tggaaatatt tatatagcag gtcagagtga taaagatgaa acattaaata ataacgacga
     5041 ttttttaatt attaaatata attcttcagg gattcaacag tgggctaaac gaaccaatgg
     5101 tttaggtaac agcaatgatc gcccttcagc aattacattg aatactgaaa atgatgtggt
     5161 tgttaccgga aggtcgttta acggaaatga tgatgattat ttcacagtta aatatgatgg
     5221 cagcactgga acagaattat ggaaaaaata ttttgaccgc acacacaacg acagagcaac
     5281 tgcaatagta tgtaacactg ccaataaccg tatttatatt acaggtagaa gtactaatgg
     5341 ggataattac gattatgcaa ctgtgtgtta tgataatgcc ggtgtagaaa tatggcaaag
     5401 tatttttgat tatgtagatg acgaccgcgc aacacatatt ggattagaca atagcggcaa
     5461 catttatgtg accggacaaa gtgatgttaa cgcgtctgct attggggtta attatgatat
     5521 cctaacagtt aagtataata gcttgggtgt tcaacagttt gcacaaacat atggtggcac
     5581 tgcaaccggt gacgacattc catcaggatt acaatttgac aacagtggga atattattat
     5641 ttgcggaagt accgatatgg atgcaagtgc cctaataaag aacgactttg ttgttttaaa
     5701 atataataca gcaggtacat tacaatggtc cacaacattt gatggaagcg gcacaaatga
     5761 taatgccaaa tcattagcag ttgacggaac cggtaatatt tatgtagcgg gttatagcga
     5821 agcagttccc aataaagatg cagcaaccat taaactcaat aatgcaggtg ctttgcaatg
     5881 ggattataac ttcaatggca ttggtgataa tgcagataat gtacacgcga taaaaaaaga
     5941 caatagcaat aatatttatt tagcaggtta tacttttggt tatgagcagg acagaaattt
     6001 tttgctgatg aaaattggtg catcaggcac tatgcaatgg caacatgtga taaatggcag
     6061 tagcaacaca caaagcaccg atgatgcaat aacacttcag ttggataatg caggaaatat
     6121 ctatgcagcc gggtttgtga aaaacagtgg cacaggttat gacatgatga ttgtaaagta
     6181 cagcacatcc ggcgattcat tatggtctta taattataac tatacattag ccaatgaaac
     6241 agataaagca attgcacttg agttagatgc ggataacaat atttatgttg ttggccgcag
     6301 tgatcaagac cccactatag tttctaatga tgacatagtt actttaaaat tatcatcaac
     6361 tggtaatttg ctttgggaaa aaagatataa tggcgcaggc aatagtaatg ataatgtaaa
     6421 agcattattt gttaccggcg gaggcgatat ttatgttacc ggaaaaacta ctaatggtgg
     6481 tcaaagtgat attattctga ttaaatataa tgcagccggt gttcagcagt gggtgaaaac
     6541 ttttgacaga agtaatggag atgacgaagc catagcatta tggattgatg catcagataa
     6601 tatttatgtt ggcgcagaaa caacaagcgc taatcatgat ttagatgtta caattttatg
     6661 ttattccaat aatggtacat taaattggca aaaacactac gactccggtt tacatggtaa
     6721 tgatgaaact aaaagtttac atgcagacaa tgaaggcaat atcattattg cggcaacaac
     6781 tgctccggat actgcattat taactttgaa cggagacatt actattttaa agtttgacaa
     6841 tacaggcgaa ttgttatggg aacaaacctt tgaaaatgaa ttgaatgatg acgccagtga
     6901 agctactatc gatgctaata ataatattat aattacaggg caaaccgaca atggtaccac
     6961 cggatataca aattatgatt atttaaccct gcagtatctt ccggacggta ccctttatac
     7021 aacagaatta tataatggac cggataatct ttcagatgta ccgaatacat taatagcctt
     7081 tgaaaatgct ttttatgtga caggaggcag ttatggtaac tcttcacaac gtgatatcgt
     7141 taccatactt tatggaacta caccggaagt agtagaaaat atccaagata atccattgca
     7201 gtttaccgtt tttccaaatc cggcaacaac acaatttcaa ataaatacac ctaacaatga
     7261 gtcaggtttg gatttatcag ttcgtttcca actttttgat atacaaggca gattacttat
     7321 agaaattccg gaaacaacat ctcccacaat aaactgctca cagctcccaa gaggtatcta
     7381 tgtgcttaac gctgtaatga atggcgaaat attatccact caaaaaatca tattaaacta
     7441 actcaattaa cttataaaat taatttttat gaacaatcga attaaaaaaa ctatcatttg
     7501 caaagaagta acaaactata ctgttaacca tgcaatcaac aactcatata ctccaaaaga
     7561 tggtgatgta gccttatttg aagtattagt tatcggaaaa cacacttctg tgcaatctga
     7621 aacaaaacgt attacagcca tttttgaagg agatgtaatt atggctgcgt ttgcaaatcg
     7681 ttatgctact tctcaatttg aaggatatac accggataca ccaactgaag tattagatat
     7741 tttaggcgca ggtggtgcaa ttgggctggt aaaatcaaaa aatgcatctt tggaagatat
     7801 tgaaccaact aaagtaagac taattggtta tgcaacaggt gttgatggca atgtaataaa
     7861 taccaaattt tatcacacca atagaattcc atttaccggt tttgtgccaa ataatgcaaa
     7921 gttgatacta tcagttggat catctatgga tagtggcaaa accacttcag ctgcttattt
     7981 agccagaggg ttaaaaacca ctggaaaaaa ggttgctttc atcaaattaa ccggcactag
     8041 ttttacaaaa gataaagatt ttgtatttga ttgtggcgcc gatgtagttg ttgacttcac
     8101 agatggcggt tacccatcaa catatatgtg tagtaaagag gaaataatgg atttatatca
     8161 aacattgtta tatatgttag aaccagaaaa tccggattac attgtaatgg aaattgcgga
     8221 cggtttatta gaacgcgaaa ctgattttct attgcgcgat accgcattta tgtcaacagt
     8281 gcatagcatg atattttcat gtggtgatag ccttggcgta ttttatggct tacaattgtt
     8341 agaacaattt ggtgttactc ctgctgttat ttgtggtcgt tttacaatga gtccactgtt
     8401 aattcaagaa gttaaagcaa aaatagatat acctacactt gacatcgatc agttaagtaa
     8461 tggggaatat gttcatttat ttgatcaaca aaaggtattt gtacatcatg cttaataagt
     8521 ttgatgtaat acgttcgttt gcggttaaga agaggggaat tattaccggc gcttttttta
     8581 ctggtgttat cagtacgggc tgctcggtaa taatcccttt atttattgga caatattata
     8641 atattgtatt tcacagtcag tcatctcgtg gtgcaatatt tgaaaaattg tttggccaca
     8701 tcgaaaacgt aacatttttt ttaatcgtat ttgggttatt cttactattt cggttggtgt
     8761 ttactttttt agaaaagtat tttataggct taacaggtga atcgtttgct aataccatta
     8821 ggcagcagtt attttccaaa caattacata ccgagttaaa cacttttgaa tcaaaagata
     8881 caggggtata tctacttcgt tacagtggcg atttaaaatc agtacaggat tacctcacca
     8941 aaggtattat tcagttttgt tatgattgtt tttttatgct tggggcattc atcctcttgt
     9001 atattttaaa cgcacaactt gcgtttattt ttgttttagc aattcccgta ttacttatta
     9061 ctaatgccat attaaataag cgcctaaaag ctattaccgg tcaaaggcgc aacttaaaat
     9121 cctgcaattt ggcatttgta gccacgaggt taaaaagtgt tttaactatt aaactcttca
     9181 accgggagtc catagaagcc gacaaataca acaaacgatc tactgagtca ttcaaaattg
     9241 gtaaaaaata tcaagcgatt gcggctttta tgcaagcgct ttatcctttt atattatata
     9301 gcagtctgct agctatattg tgggtagctt acctacaagt aaactcagac actaaaacca
     9361 ttgatggttc cacattagtt acctttatta tgttggtgat tagtataatt cctgtattca
     9421 aacgtttgtt aaaggtaaac atgatttggg tttcaggtaa tgtatctttc agcaaattgt
     9481 tacttttatt taatgccaac gaagaaaata aagctaaaac accaaaaata aatcgcatta
     9541 aagggtcctt aaaagccgaa gatatctcat ttaaatatag caatgatttt gttctacaaa
     9601 atatcaactt cacaattgaa ccgggcacaa taactgcttt agtgggaccc cagggttccg
     9661 gaaagtccac tttatttaaa ctgattaccg gtttatatcc tcttgataaa ggcaaacttt
     9721 ttttagacaa ctatttggta gctgaccttt ctaaacatat cctccgtaaa aacatcacca
     9781 tagtaagcga caatttgcca ttgcttggta gaacctattt tgaagccata tcctatagca
     9841 gacaagaaaa aaacagagct gcaggtaaag cacttttgtc agaactagga atattaggtg
     9901 gttcagaaaa tgatgtttta aataaacaaa ttatagatgg aggaagaaat atttccaacg
     9961 gagaaaaaaa gctattaaat atagcaagag cacttttaac taacaaaaaa atcatactaa
    10021 tggacgaacc tttttccgat ttaagtccaa aagctattga attagtatgt aaatgtattg
    10081 agaaacgacg tagcaaacac accattataa ttgcagataa tttaaatata aatattgcgt
    10141 atgacaaccg gattaccctt catgaaaaaa tcagtaatta aaataatctt gattattgtt
    10201 tttgcaacaa acctttggtc ttgtacaaaa gagccgggaa ttggcggaga agcgactatt
    10261 tcagggaaag tatacgccaa acattataac tcaaccttta cagtgttatt aagtgaatat
    10321 tacgttcctg acacttatgt gtatttagta tttggcgatg atgttaatta tggcacacgt
    10381 ttaaaaacaa attatgatgg tgagtttgag ttcaacttcc tctacgaagg agaatatacc
    10441 atatacacct actctattga ctctgccgct attgtaaatt ttgctgttga cccaccggag
    10501 acagcaatta tagaaaaagt taacattaat agtaaagatg ccaatatcga tattggcgat
    10561 ttaatcattt acaaataagt aaaaatgaaa tacctgttaa ttctttttac cattttaagc
    10621 attggtgtta agggctatag ccagtccggc acagcaagag gtgcccgtgc cttatacaat
    10681 gaagtatccg agttgaaaaa cgggcaattg caaattgtta aacgtaccta ccaggaattc
    10741 gacaaaaaag gccgagttac tttagaagtt gcatttggct tggatagttt aatgaaatcg
    10801 aaagaaacct ttacgtatga caaaaaaggg aatgaaacaa tacatactat ttataattcg
    10861 gcaaatcaaa tcgaaagcaa aattcttaca acctacaatc ggtttaagga aaagaaaaat
    10921 ataacagttt attcaggtac agattcatta cttgaaaaaa caacctttac atacgataat
    10981 ttcggaagaa aatcacttga attaagtgtt gacgccaata ataaacaaat aaagaaaatc
    11041 accttttctt atgaccagcg gggtgcatta aataaaagag taatttatga tggagctgac
    11101 aatatcacac aaataaaaca atacaattac acctattcta caaaatgaaa ttgtattcgt
    11161 tgctgatatt atttttattt tggcctttgt tgcaatttgc gcaacaacag gatgctattg
    11221 gttcattcgc ctttcaatta gagaaaaaac tcaaccaaac attttctgta agttattact
    11281 cccaggttgg atttaatgaa aacataactg aatatggtta ttccctatac gatttaggtt
    11341 taaatatcaa attaacggat aatttttatg caggttataa ttaccgaatt gccaatatta
    11401 aatcaaacga aaataaatac ttcgatcgac aaatgatgta tgttgatata ggttgggtaa
    11461 agtcatttgg taaattcaac ctgaatatcc gtactcgtta tttaaataag tggtatggat
    11521 ggaatttcga tgaaaacaac aattacaaag acaatgctca ttatttcaga aacaaaatac
    11581 aattaaaata tgcaattaac tacaattatt caatttatgc cggttgtgaa caaatatata
    11641 gactggattt aaggaacgaa accgagcaat taaggtatgg tggtggtatt aactatcaga
    11701 taaattcaaa aaacaagtta caactatcct atatcatatc acgcgagcaa aacagggcaa
    11761 aacctgatac taattttact acaggcttaa cttattattt taagttttaa atttgcgtta
    11821 tgaagtcatt atctattgca gcctttgtag gactgtttag tctacttttt attgcttgta
    11881 aagagcaggt tgaaaccagt cagccaatac aacaaaaagc agcatttgca cttgccattc
    11941 acggtggagc cggggctatg cctaagggta gttacacacc agaacaggaa gcagcttttc
    12001 acgcaaaatt gaaagaggcc gcacagttag gttatgaaat gttatcgaga ggtgacagtg
    12061 cggttgctgt ggtagaggct gtaatttgtg ttttggagga ttctccatta tttaacgcag
    12121 gcagaggctc tgtttttacc aataatggaa aaataaaaat ggatgcttct attatgaatg
    12181 gcagaaattt ggatgcaggt tcaataagta atgtgcaacg tattaaaaac ccgattaaaa
    12241 ctgcacgaat ggtaatggat tcatctaaat atatcatgtt tagcagtgca ggtgcggagc
    12301 gttttgccga aaaatataaa ttggaattag ttgatgtttc ctatttctat acacaacatc
    12361 aatatgaacg ttggaaagga atgaaggata gcactgaagg taattacatc aaatatgtag
    12421 attcagtaat ggctttgcag gaagagccgg ttgcactgac caatattatt gaaaaatacg
    12481 gaactgttgg ttgtgctgtg ctggacaaat atggtaatct tgcagcagga acatctaccg
    12541 gaggtttaat gaataaaaaa tataatcgta ttggcgattc tccggtaatt ggtgcaggaa
    12601 catatgctaa caataaaact tgtgctatta gttgtactgg aacaggtgaa gattttataa
    12661 aaactgtcgc tgcaaaaaca gtatcagact taatggaatt taaaggattt tcacttgagg
    12721 aagcaaccaa cgatatgata catgtaaggt ttaaggaaat aaccggtgat ggcggaatga
    12781 ttgcagttga taatttgggt aacatttcat tccaatacaa taccgatggt atgtttagag
    12841 ctaaagtgga cgaaaaagga aatatttcta cctacattta caatgaataa atttttatta
    12901 attgccggtt tacttttgtt atttgcctgt aataacgaaa aaacacaaac acctgtagcc
    12961 catagtaaaa aattcactta tcagttatcc caggcaacta aaatttgcag acaagaggac
    13021 tggtttgatg aaagtggaaa gattataaaa acaattcact atggcaatag ggagaatgat
    13081 acattaaata ttaccaatta ctattacagc ggcgatttac ttgttgagca attacgcata
    13141 aaaccggatg gaaaaataat ggataaaact gtttaccatt atacattagg cagcttgaaa
    13201 ggagaatcgt ttataattgg agctgataca cttaccacta aatatttcac ctattacgat
    13261 aacggtagtt taaaaagaac aactacggtt tataatatgg agaagaacaa tccggtaact
    13321 acagtaacct atttagatga gcatggaaat gtcgaaaaaa tttacaacca gatttatgag
    13381 gacagcacca aaatagtgct ttccaggtat gaaatgcaat cctataaaaa tacttacaac
    13441 agtttaggga aattggaaag cagcattggt accatgttat ttgggtatta cgaaactact
    13501 gacacaatat ctatcacaag cttcagatac gatgcttcag gcaatatgac acagcagtta
    13561 aatctaaaaa aaacaaatag cggggagccc gatagtattg tttatcaata caacgcttca
    13621 aacatactta ctcaaaaaat tgcttacata acagccaaac aaaaaaatga agtagtttca
    13681 attattgaca ctacttcata tatatatgac actaacagcc gcttaattaa agaattctca
    13741 tcccattata ataaagggtt tgaatacaaa tttgaataac aacgttaaac tatatttcaa
    13801 taatactaga acgttgttta agttgatttt tattaagcta taatttgttt taattcaatt
    13861 tccataacta ttattttcaa ggtgaaacta ttttagtgcg ttcatacgtt attaccttaa
    13921 aagtgtaata ttgccttctt caacctggtt gccattttca tttatatagg aaaccacata
    13981 aacatatgtg cccattggtt gattttcatt tttaaaagta ccattccaag ccgtttcaat
    14041 attagtagtt tcaaatactt tttcccccca tctattataa atatgcaagt aggtaaaatc
    14101 aatgtcgcaa gcggcaatta atctaaatac atcattagcg ccatcgttat taggcgaaaa
    14161 tgcattaggt tttagcaatt cacaattaca ctgctgtgct ataacaagca ctgaatcgat
    14221 tactgcgcca caatatccac tatcggttac ggaaaataat ccaggctcaa aaattgtgat
    14281 ttcacttcct gtttcgcctg tactccaggt tgctgtttca ctaccaacag ttaaaaccaa
    14341 actttcgtaa ttgcataaaa ttgctgtttc aggtaaattt atttctgctg gagatggcaa
    14401 aactgttatt atattttcca ttgtaattgt gtcagctccg gcatcatttg tcacaattaa
    14461 tgtaacatta aaattacctg cattttcgta acatattacc gggttagctg tggtagcaac
    14521 atttggagtc ccaccttcaa actcccataa aaaactactt gcacctgacc ctgtataatt
    14581 aaaatttaaa catgatgttt ggcatattgt atccaactca tttgtgaagt tagctattgg
    14641 caaaattgcg gtagtatcgc aaaatttggt tatccaataa tcatccttca ggaatgcata
    14701 tggtgattga gttttatatc cggaaaccgg tgcgtaagaa taagttgcaa acaaataaca
    14761 tccatcttgc aattgaatac ccaaacctaa ttcctcatga ctatcggtaa agatagtttt
    14821 atcccaaagc acagttccat tgttatcgat aagtaaactc cacatctgtt cttcgccaag
    14881 gttttcttcg gttttttcac cacttggcca tgagtaggaa tttccgctta acaaatagcc
    14941 gttgtcgctt gtttgaaaaa tattaccaaa ttcatcctca aaatcttctc caccatatct
    15001 gtgatcccaa attttattcc cgtccaaatc aatttttaca atccagtagt ctgtactgtt
    15061 accataccat ggatgcagat acgcaggttc cgttttatca ccactaatat ctgaacgcga
    15121 gttaccaccg attataatat tgttatcgtt atccaaaatc atgtggtaaa ctttctcctc
    15181 taaattgcct ccatattttt tgtcccatat ataattacct tcagcgtctg tttttaccac
    15241 ccaataatca cctaaacctt cctgatttgt agttttatca ccactaactc ccgaatagga
    15301 atatccaccc aacattagcg acccatcagg taattccaca attccatgca ttaaatctgc
    15361 ttcaagacca ccatatcttt tatcccaaag tttattgcct acagcatctg tttttacaat
    15421 ccaataatca tattcatagg aaccatcttc ccaggcaggt tgagttttat caccattatt
    15481 atcaccttct gtataaccac ctaatacaaa tcctccgtct tgggtttgta ctatacacgt
    15541 caaattttca gaagcagttg taccaaatct tttatcccat aaataattcc catctgcatc
    15601 aattttaatc atccaataat cgtaataacc ccatgaaggt tgtgattttt cgcccgaaac
    15661 tggggagctg gaaataccag ccaacataat actgccatcg gctaataaca cacaatcata
    15721 agcagcgtct gcatttgttc caccgtagcg ataatcccaa attttttcac cgaatgcatc
    15781 caatttaagt acccaaacat cgcctgaatt tattcccggc catgtttcat aagcatcttg
    15841 tgatttatca ccttcagcat cagaacttga agtcccatat aaaaagattt caccagaggg
    15901 caattccaaa acaccatcta aattatcata ttcagtaccg ccgaatcggt aatcccaatc
    15961 tttttcaatt ggagcctgac caattacgac ggatacactg aatattatat taaaaccaac
    16021 tatcaaatat ttatttaaca tttcaaagaa attattcagc tttaattaat tggtatgttt
    16081 gaacagaacc tgcattttcc aataccatca tatatgctcc cgggagtaat tgctgaccca
    16141 gttctatact ttcggttcct gcttcaatgt ttgtactata aaccaattta cctgtaatat
    16201 cataaatagc tagttgaaca ttttcattta cattagcaag ttgtaattga gtagcagtac
    16261 caaatggatt tggtgaaatt gtatattcca tttgattatt tattacttca ccatctttac
    16321 aaataattat ggttacacta atggtattag atgttttagt gcaaccgttg ctattggtta
    16381 ccacaacttt ataagcacct gcaccttttg cattataatt tgaacttgtt gctccggcaa
    16441 tattagctgc tccttttttc cattggtacg ttaatccggc accaatattt gcactcaata
    16501 aaacactgtc gccgatacaa atacttgtag caccgcctgc agttatagtt gcctccggaa
    16561 gtggcgtgca aaattttact gtcgaaaaat catagttggc agtgccggta ccaacgctgt
    16621 aacctgttat atataaatat ccattgttat ctaaaattac ctgcttacct acatcttcgt
    16681 tgttgccggt gccattgtat tttaatgtca gtaattttgt tcctgccgta ttataattga
    16741 gtgcaacaaa atcagcaaca gtagctgcgt taccggtaac tccaccagtg taaatattgc
    16801 caccaccatc ggtaacaaca ttatatcctt cgtcatttaa agcgcctgct cctgtatatc
    16861 gttttgccca attttgagcg cctgtggatg cattgtatct gattaatgca atatcatttg
    16921 cactgccaac tcctgcactt tttcctgtaa cgtaaacatc tgtagcagta gctgaaacac
    16981 catatgctct atcttcagca tcggcagtac cattatatgt ttttagccat aactgattga
    17041 gtagcgtatc atattttata gtaacataat cacctaaatt gcctgttacg aactctgttc
    17101 ccgttacata tactgaagcg cctgaaataa acatatcttc accctcatca tctccacctg
    17161 aacctgcacg agttgcaata gctaattgcg ttcctgcatt atcatatttg atggtaacaa
    17221 tatcattatt tgttccatta aatgcatatc ctgttacaaa tattttatcg cccgacaata
    17281 ccaattttgc agctttatca tcaccgtttc caacaccatt ataccttgtt gcccactgta
    17341 aaatacccag cgcattatat tttaatgtta tccaatcata attggtgata agcgggtcaa
    17401 cattactatc gctgtagccg gttacaaaaa tatcacctgc aggatttacg gcaatactca
    17461 caaatttatc gagttgactt gcaacgtaat tatatattat attgagttgt aaaacacctg
    17521 aagtattata tttcaaaatt aaaccatcat cgtatgttcc ggaaccgttg ctataacctg
    17581 caacatatac attacctgca gcatcagttg tcacagccaa accttcatca ttttcattgg
    17641 tgccatttac attgcgtgac caaagagttg ttcccgctga attaattttt tgtactaaca
    17701 catttctttg ttgtgcttca acaagcgtat atcctgtaac aaataaattg tttgaagcat
    17761 ctgtagcaat tgcttttgct ttatctgtaa aatcaccttt accattatat gtttttgcta
    17821 tttgaaaaac acctgtgcta ttatatttaa taaaagtggc atctttttgt gttgcagtga
    17881 aactaacacc acctgcaatc cagacatttg atgcagcatc tacaattatt gttgatggaa
    17941 tatcttcagc atttgctact gttccatcat aatatttcaa ccattgttgt gcgccggctg
    18001 aattgtattt tacagtcata aattcaaaat taactgctcc acctgcaatg gcatcacttt
    18061 ttcctgtaac ataaacgtta cccgcagcat caaccaataa atctgatgga acatcttcct
    18121 gattggcagg attaccggtt atggcactcc attgtaaaac acctgcacta ttatatttta
    18181 tcgttctaaa attataatcc gaattaccac cggcatcaat atccgtttga ccggtgatat
    18241 aaacattatt caacgcatct gtttttattg ttgttaaacg gtcattgtcg cctccattta
    18301 caattttaga ccattgtaaa acaccggcag tattatattt aagtgtaaca aaatcgtcat
    18361 cgcttgcatt tgcgcggatt ccaccaacat aaatattacc tgtagcatca agtgcaattg
    18421 cctgacctct atcgtcacct cccggtccat tgtataaaac tgtccaatta acagttcctg
    18481 tagatgaata actaatggta agaatatctt cacttcctcc ggtatcagtt ctacctgtaa
    18541 tgtagcagcc accggcatta tccgcaacaa ttcctgctgc tctgtcggtt aatccaccat
    18601 catattgcgc agcccattgt tgaacaccaa ggttattata tttaatggta ataacgtcat
    18661 caatattacc tgcatcatga tcacttcttc cggaaataaa aatattattt gcagcatcaa
    18721 cacaaagtga tgcaggaaat tcatcttcat taacagctgc attattatat cgggttgccc
    18781 aaagcaatgc acccgctgca ttatattgaa tagttaaaat atcattttta gttgaagttg
    18841 atcctccgtc ggagtaacca gaaacggtga tatttcccag tgcatcaatt acaataaaat
    18901 ttgcttcatc atcgaaatta gcaacataat tatattttct tgtccaaaca gtatctccgg
    18961 cactgtttaa tttaacagtc aggaaatctt ttccggttcc cgggcttact gtatatccgg
    19021 ttaaataaaa atttccggca gcatctttta cagcgccatt gtagcgatcg gcattatcac
    19081 ctgagccatt taatctgttc ttccaaaggg tagtttgtcc tattgccctg gtattggaaa
    19141 gaattaaaaa catcatcaca atcataattg cgactagatt tggtaatgag attttgcgca
    19201 taactttaat ttgatagttt aagtttttcg ctcaataaga acttgagcaa tgtaaaggtg
    19261 tgagagaata attgcctaaa aaaaaacaga taaaggctgt ttccaagtcg tagtgatggg
    19321 aagatatatg tagatacagt taattaagtg ccatatcaag gataatcgtt tgattatcaa
    19381 cattttagaa ttatgaaatt agcgacactt ttgcggattt ttcgccaaaa acgggcacaa
    19441 aaaacctccc aaagcttacg ccagggaggt ttaaagttca agtatgaaaa gaaaagatta
    19501 tactaaactg caaaactttc tccacatccg caagttctgc ttgcgttcgg atttacaaat
    19561 tgaaagcctt ttccgtttag tccatcggaa taatcgagag ttgtatcaca caaatagagg
    19621 aaacttttga ggtcggttac gagtttttcg ccgcggtctt caaattcctg atcaccggct
    19681 ttagcttcgt tatcaaag
//
LOCUS       DAORKW010000015        19323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_24_length_19323_cov_5.271383, whole genome shotgun sequence.
ACCESSION   DAORKW010000015 DAORKW010000000
VERSION     DAORKW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19323
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24_length_19323_cov_5.271383"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1945)
                     /locus_tag="PLB46_01425"
     CDS             complement(<1..1945)
                     /locus_tag="PLB46_01425"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRF75199.1"
                     /translation="MFKIQLPKAITTLFTTAIACLSISYASAQDTILEQSFDFPALFP
                     PFNWTNTKVAGTGAPGNWARVTTGGSPIQEPHSDPAEAKFNSFYYPAGTACDLSSEVV
                     DLSVSGTYTVNFWMYRDAGNGNDKLEVYVNTTQQSAGGTLIGTIFRKKTISPVAATEG
                     WYNYSFTIPASYNTTANYITFKAISDYGFNIYLDDIAIVKNVISIPSCATGNSPVDGA
                     IGVCSNATISWDIVPYATGYKLTMGNNAPNYNNVASNLDLGNVQSFSALLNNSTVYKW
                     RVRPYNENGNAVGCVQNTFTTASDVCYCLPEFIEPNCVSLDFIDDVYTTGALGNISNM
                     NTGCAGDDNNYTYYSALTIVAVKGGTFTLNMQSGIDYAQGYGVWADWNKDGDFDDAGE
                     FVYQSTTPTTALVSAAITVPETATLGVTRFRIRAFFNEAPLATQSCAVWNEGESEDYN
                     IEVQECTVTDYYFDGDGDGYGNPFAVFTTCVPPVGYVINNTDCDDANSSVNPGAPEVC
                     NGFDDNCNVTIDEGAVTATVTPAGATTVCKGAPLLLNANTGVGYTYQWTRNGANLAGA
                     TASSYNVTKSGNYAVKVTAPGGCIATSASVGCTVTSNPNANISTPEGTDLCGISDLDL
                     VANGGVGDNFIWDKNGVGIGGAPT"
     gene            complement(2197..>3918)
                     /locus_tag="PLB46_01430"
     CDS             complement(2197..>3918)
                     /locus_tag="PLB46_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF75200.1"
                     /translation="FCEAPVSGWSLTTGDCDDADDTVYPGVIDLCNGIDNDCDGNIDD
                     DALFTTYYLDNDGDTYGDQLDAGTTFCYVPAAPYVLDNTDCNDGDMSINPGATEICNA
                     MDEDCDGIADDGLTFVTYYKDNDGDGYGAGLYGTVTTCDGAPATYVDNDLDCEDFNAA
                     INPAADDVCNFIDDNCSGVADEGLPIFTFYLDADGDTYGNPGATLEACGPFGVMWVEN
                     SEDCDDADADVHPGVIDECNGIDNDCDGNIDDDALFTTYYLDNDGDTYGDQLDGGTTF
                     CYVPAAPYVLDNTDCNDANNTAYPGAAELCDGIDNDCDGSTDEDLVTASVSPAGPTVL
                     CKGDFIELTANAGPGYMYQWYKNGNLIPGATSISYSTNKSGYFQVEVSLAGGCSVLSA
                     THGISVIAKPNANITAPNGTSLCASVKLKASYGGGYSWQWYLDGSPIPGANDYLYFPT
                     VAGSYACRVTNASGCSRNTPAIAVTACKEGELVDGVITETFDIYPNPTIGEFVIDMTL
                     NTTATSADMQLLNIMGEIVYNNSVSVNNGSINESILLNDNVPSGMYIVKILVDGSEYT
                     KQLVLQK"
     assembly_gap    3919..4018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4019..5741)
                     /locus_tag="PLB46_01435"
     CDS             complement(<4019..5741)
                     /locus_tag="PLB46_01435"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HRF75201.1"
                     /translation="MNLKFTLKAGLRTLVVLMLFVSSNVFGQSGIYESYAVLNINGAG
                     NTFYDMQAITGNTDLQGANLGTFTIGAGNSLVIAGGENKTFKCSPCDITNGNLWYRVW
                     SGSPSGSFTNVGLSFNSNLGTGCGGNDQKWQTLSGSTNVLTGLPAGSYTLEVYSTADY
                     AGCGTGTHYSSNGGANYQATFTVNCPVITVNESHVDATCVGGSNNGSIDLTVSGGTPF
                     TTTITQTYTQNFNTLASTGSPSWSDNSTLTNWYSNRIVYFPNTGTNTTGGLHSYGSAA
                     VPADRALGSLGSGSANPVLYAIQITNTTGETVSSVTVSYTGEQWRCGGSGAVANTLDF
                     AYQKNAASITTGTWTDVNSLDFTGPVVSCGTAGALDGNLPANRISFNQTFSVSILPGE
                     TIWLRWNDPDNTSSDHGLAVDDLTVTLTGPTINYYTYSWSNGATTQDISSLTTDTYSV
                     TVTDANGCTGTNNIFVDLTGQSVYYADSDSDGYGDNDVNETACSPSEGYVANNTDCDD
                     TNNAINPGETEICNGIDDDCSGVHDDGGVVFLDYYVDADGDGYGQGAAFGFCEAPVSG
                     WSLTTGDC"
     gene            6145..7542
                     /locus_tag="PLB46_01440"
     CDS             6145..7542
                     /locus_tag="PLB46_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75202.1"
                     /translation="MPIIFQLLKTLSPSETDQVNALPFQDRERDVLQVMLQMNNRIFP
                     SSTVCKRLGITTSHLDKINSILFKKVIEAVAGNNIYHQINYLDQKNGLWKAALLILKH
                     HEHKVIALSTDKTEKFEFYKFYFEWLLFNTAVQNIDEEINRLNDEILNNCPRSTYNET
                     QLWLKISIFRKEINISTTKADFTDPEKQKALFLKLNNLISEAQAIDSAHCLYKAKLCG
                     VFLNNLLHNFSQSRIYISKINDLFLGYKEAFTETEILTAKWHYAQILFFSSEFEEAFN
                     IYHSLYGRINKNEPLRWYIYIAEYFQICLVVERYDLAEEICDNYFAKFYKDSNGNFFL
                     SALIQCVKLLIHTSKFDEAKSKIDELHRLTTKTSSLQFQFALRELTAAYHYLTKDYKT
                     ALTLAEKNLKFMRSKKMHVLIPEYTYHSRLIKVIIKNKQKNRQFDPEEQAMFDEMQKS
                     TLAQYGHLLKRLIAI"
     gene            complement(7608..9386)
                     /locus_tag="PLB46_01445"
     CDS             complement(7608..9386)
                     /locus_tag="PLB46_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF75203.1"
                     /translation="MKNNYLLSLKSLFTASVMFLATGVFAQTNPTPQTIPYFEDFSGL
                     THASTTYPMGLQGWKVSSAATTVVITGGPTADHTLTANSSASSNAGGVHNYNGKIGTL
                     ASGSTNPGLVLALNTTGYNNITLSYDIMVIRNPYDGASNTRLNEATLQYRIGTTGAFT
                     AITGVEYQSGTTQQTASGVTTPQDLQARSIVLPVACENQAEIQIRWITHDMTGAGSRP
                     SFAIDNINVDADNDGDTFLSTVDCNDNDATIYPGATEICNFIDDDCNTITDDGITYLA
                     VYDDLDGDTYGAGLAANYCTFPGAGYSLDNLDCDDSNVAINPAANEICNGVDDNCDGN
                     IDEITVTASISPAGVVTTCKNVGTTLTANSGDGYTYQWFKNGNLITGATDVNYTTSKP
                     AYYQVEVSAPGACPVLSEFVEVNIAPNPNANITTPNGLNLCLGTVKLKASYGVAYTYQ
                     WYLDAIEIPGATDFIYLPATTGNYTCLVTNEFDCSRLTNMVTVINVCKQGEDVAMEIQ
                     NEKLVIYPNPVSNVVTIDVTTSSINTSATIMILNVLGEVMYSEHHAVTNGALNANLNV
                     NNLSNGAYVVAVNINGEIFTTNMVVE"
     gene            9662..10561
                     /gene="gldA"
                     /locus_tag="PLB46_01450"
     CDS             9662..10561
                     /gene="gldA"
                     /locus_tag="PLB46_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HRF75204.1"
                     /translation="MSIIVDQLTKVYGEQTAVNQISFSVQSGEIVGFLGPNGAGKSTT
                     MKMLTCFIPQTSGKASVCGFDTEKNSLDVRRNVGYLPEHNPLYPEMYVKEYLEFAARL
                     NGMKNADKRIEDMIAMTGLTLERRKKIGQLSKGYRQRVGLAQAMLHDPKVLIMDEPTS
                     GLDPNQLAEVRSLIKNIGKEKTVILSTHIMQEVQAICSRVIIINKGKIVADDSVDGIQ
                     AQQKGTTIIQVEFKEAVNHQLLKQIAGVRSVKGDTKLVIEYYGDADIREYLFRFAVEN
                     KYTLLNMHIEKQNLEDVFHDLTK"
     gene            10592..11323
                     /gene="gldF"
                     /locus_tag="PLB46_01455"
     CDS             10592..11323
                     /gene="gldF"
                     /locus_tag="PLB46_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HRF75205.1"
                     /translation="MIAIFRKELNVFFSSLIGYIAITIFLIANGLFAWVFPETNILDD
                     GYATLDSFFNFAPWILMFLIPAITMRSFAEEKNSGTIEFITTRPITDMQIIFGKYLAA
                     LVLVIFAILPTVLYYFTVSALASPAGNVDTGGIIGSYIGLILLGATFVSIALFTSSIT
                     NNQIVAFIAGMFACFFFYLAFDSLSLIPAFIGTADKYFNAISINRHYLNISKGYIDLR
                     DVIYFSSMIGIFILMTKTALGSRKW"
     gene            11352..13088
                     /gene="gldG"
                     /locus_tag="PLB46_01460"
     CDS             11352..13088
                     /gene="gldG"
                     /locus_tag="PLB46_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HRF75206.1"
                     /translation="MERQDQYKKNRRRAMLQLLLLVGIIVIINVLLSGFFFRVDLTEE
                     KRFSISEPTKTLLKEQEDILFIRVYLAGDLTPNLKRLQQATTEMLDQFKNYAGDNVQY
                     DIFDPFSIPNEENQAEFIKQLEEKGILSVQFMESATDEASIKYIFPFISINYKDSEIA
                     FPMIDRGTMPLPLNPDSDPSVSISLLEYNFTKAIRQITDADKPYVAFIAGHGELNRFE
                     LNDIAGSLNELYNVTQIDLEDDSTYEIPKEIKTIIVAKPMYEFSMEGKFIIDQYIMQG
                     GNVLWLVDPVIADFDSLYSGKGQFMAIDRELNIMDMLLQYGARINTNIIQDKQCTHIN
                     VPVANGENFIMRPWPFNPILNNFNGDHPISKNVDAIEGKFVSTVDTIAVPGITKTILL
                     TTSGLSRYLVAPARVNFNVVDTKFAPTDEQYNKPNQPVAVLLEGQFPSAFKSLKPTAN
                     RLHQMGFAYQDGKVLEKGTGSKQIMIGDGDLIKNFVDENGKPDMLGINYIERYIYGNK
                     DFAMNCIEYLCDQNGLIATRAKEVKLRPLDEQKVGENRIQWQLVNMIAPLLVLYLFSG
                     IYFFVRNRKYAA"
     gene            13101..14144
                     /locus_tag="PLB46_01465"
     CDS             13101..14144
                     /locus_tag="PLB46_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRF75207.1"
                     /translation="MNRNWLYFGILVILAVIAYFTLIRDNEGSYSKKDTAFAVEDTTA
                     IGKILLTNLKGDSILLEQNNGSWTMNKDYRPRPDAIHNLLNTMVQLEVKMPVAKSMHN
                     TVVKKIAGKRTHVEIFNSKGEKTKGFYIGDNTDQLNGTFMLMENSDHAFVVNIPGFAG
                     FAATVFFTDETDWKSKNIFAYKPDAINQIDITYNNLRDSSFSIVRKADNTFELIGNNT
                     INKPLNPEIVSYYLKQFGMLNAEYFILEPEKRDSLLATTPACIMSVTDNNKITTTLKI
                     YYRPLTYRSKMQYTLDDKPIEFDLDKYYGVFNEDKDLGIIQNFVFGKLMVGPQFFYRQ
                     RPGNVSVLNEGLK"
     gene            complement(14186..15763)
                     /locus_tag="PLB46_01470"
     CDS             complement(14186..15763)
                     /locus_tag="PLB46_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75208.1"
                     /translation="MNRIALVMSVLFCFQIAQKPLLSQVTAPPFTITLEEVTYSDWPG
                     IHSFALGEWDGRWLIMTGRTGGLHGFLPPTPFPIIDANKQIHLFDPETGDYQSANLFT
                     LPEAVQDQLQSTNAQFFQRGKYLYIIGGYGHQQSTDSMITYPSLLAVDLEMMSNLIDA
                     NESVAPAIRSLTDTLFEVSGGEIGLIGDTIYLFGGHIFAGEYSKPAGPQFFQEYTDAL
                     KKFTLADDGETITIQDITIIKDTTVFHRRDLNFEQIMLPGAEEALAAFSGVFQYEGDW
                     VWFSPVYVTTTGYSEDEAFYQKLNHYTCPVMSIYDSVSQNYYATLFGGIGQYYYDEED
                     DTIKQDLNVPFVNDISTIIKYADGTTEQYPQPIQFDALLGSNAEFILNNAIPHYNNRV
                     IRLHDINGSVLAGYIYGGIDALIPNFTPSTASNRLFKVWINYTAPVAIQNVSINDISI
                     YPNPVSGEIIISNSSLKNIEQIQIINQLGETMPLLQAKCMAGSSITIDVSVFAPGLYI
                     VKMFGEDNVLTKQLIKL"
     gene            15850..16968
                     /gene="dnaN"
                     /locus_tag="PLB46_01475"
     CDS             15850..16968
                     /gene="dnaN"
                     /locus_tag="PLB46_01475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRF75209.1"
                     /translation="MKFIVSTTALLRNLQTVNGVVSPNTVLPILEDFLFVVEKNKLTI
                     SGTDLETSITTWMDIESKSEGKVAIPARILVDYLKTLPEQPLTFSIDEDSCQVEITTD
                     RGKYKLNGENGDDFPRVSSDDKMSDITLPSGVLQKVINKTAFATGTDELRPPMTGVLF
                     QLNKDGINFVATDAHKLVRYRRTDAKASGSASLIMPKKALNLLKSNLPGEQTQVTISY
                     NSSFAFFSYNNVKLICRLIEGRYPDYNAVIPTENPNRLTINRADFLNTLRRVIIFSNK
                     TTHQVILKIKGNELIVNAQDLDFSNEANERLDCTYEGDNMEIGFNAKFLLEMLNAMDN
                     EEVRMELSTPNRAGLLLPTESDPAEDLLMLVMPVMMNV"
     gene            complement(17301..18509)
                     /locus_tag="PLB46_01480"
     CDS             complement(17301..18509)
                     /locus_tag="PLB46_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75210.1"
                     /translation="MKTFLNLIIAITTTCLFVRCDNDDVTLSSENIPLASAEEIKTWE
                     LTTETLLGIRAYDPTKGELSTMEQHTALTTDVFGGLPFYKPIFNALSKEKQNIGGTLN
                     RYHSHAEDNNSNDINLWMILFEEYQQFYDIVAFNKNDDWLFPTTLNGPELTCGDYDKN
                     VPFLLGEIAVPNSFIDNNFFFRRADNTYCESGIYLHKLDTFGMYGAVTTDRHHGNVPE
                     VHPAQQFWFRNKTVNAKNGEGYWLFFVQDASDRFVDWIGSPLHGQYKIAFRYKPNKIK
                     AHTLGPLTMDISLDLKEDMVTDKFPDQFADCDNGRTHGLVVDGKRIVSVTESGFNDND
                     LGIQFVDVRKLSDGTIQGYVQVSIVIGDVETDAFGMGILSLTIKQPQSTLVNQPTGDL
                     PVKENTKRKE"
     gene            18835..19302
                     /locus_tag="PLB46_01485"
     CDS             18835..19302
                     /locus_tag="PLB46_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75211.1"
                     /translation="MKSKLTTPFIAIGTILLISSSCEKTTEVDLNAEYLGKYRIEYTY
                     EEPYFNGTDWENNTITNIDSTCELTKDANGDLLFTNFDFFWDLPMKTYIESDSICIPQ
                     YDYYLGGPYPNTYKAYGNVVDGELVLEINRTLYDWTFMISLNQPGTAVATKID"
BASE COUNT         6296 a         3569 c         3395 g         5963 t
ORIGIN      
        1 gtgttggagc gccgcctatt ccaacgccgt tcttatccca aataaaatta tcccctacac
       61 cgccgttagc aactaaatcc aagtcggata ttccgcataa atcggttccc tcaggagttg
      121 aaatattagc atttggattt gatgttactg tacaaccaac gcttgcagat gtggcaatac
      181 aaccacccgg tgcggtaacc ttaactgcat agttacctga tttagtaaca ttatacgatg
      241 aagcagttgc gccggctaaa tttgctccat tacgtgtcca ttggtaagta tatccaacac
      301 ctgtatttgc atttaataac aatggtgcgc ctttacaaac tgtagttgca cctgccggag
      361 ttacagttgc agtaacagct ccctcatcga tagttacatt acagttatca tcaaaaccat
      421 tacatacttc gggagcgccg ggattaacac ttgaattagc atcatcacaa tcggtattat
      481 taattacata acctaccgga ggaacgcatg ttgtaaatac tgcgaaagga tttccatatc
      541 catcaccatc accatcaaaa taataatcgg taactgtaca ttcctgtact tcgatattat
      601 aatcttcact ttcaccttcg ttccaaactg cacaagattg tgttgccaaa ggagcttcat
      661 taaaaaaggc gcgaattcta aaacgtgtta cacctaatgt tgctgtttca ggaactgtaa
      721 ttgctgcact cactaaagcg gttgtaggtg ttgttgactg atacacaaat tcgccggcat
      781 catcaaaatc accatcttta ttccaatcgg cccaaacacc ataaccttgt gcgtagtcga
      841 tacctgattg catatttaat gtgaatgtac ctccttttac tgcaactatt gttaatgcgg
      901 agtaataagt ataattgtta tcatcgccag cacatcctgt attcatattg gagatatttc
      961 caagtgcacc tgttgtataa acatcatcaa taaaatctaa tgacacacag ttaggttcta
     1021 taaattcagg taaacaataa caaacatcag atgcagttgt aaatgtattt tgtacacatc
     1081 caacagcatt accattttca ttatacggac gaacacgcca tttataaacg gtgctattgt
     1141 ttaacaatgc agaaaaactt tgcacattac ctaaatccag attagaagca acattattat
     1201 aatttggtgc attgttgccc attgttaatt tataaccggt tgcataagga acaatatccc
     1261 atgaaattgt tgcattagag caaacaccaa ttgctccatc aaccggagaa tttcccgttg
     1321 cgcaactagg aatagaaata acatttttta caatggcaat atcatccagg taaatattga
     1381 atccgtaatc tgatattgct ttaaatgtga tataattagc agttgtatta taactggcag
     1441 gaattgtaaa actatagtta taccatcctt cggtagccgc aaccggggaa attgtttttt
     1501 tcctgaaaat tgttccaatc aatgttccac ccgctgattg ttgtgtggta tttacataaa
     1561 cttctaattt atcgttacca ttacctgcat cacgatacat ccagaaattt acggtatagg
     1621 ttcctgatac cgacaaatcc acaacttctg agctgaggtc gcaggcagtt cctgcagggt
     1681 aataaaaaga gttaaatttt gcttcagcag ggtccgaatg tggctcctgt attggtgaac
     1741 caccggttgt tacccttgcc caattgccgg gtgctccggt tcccgccacc ttggtattcg
     1801 tccagttaaa gggaggaaac aaggcaggaa agtcgaagga ttgttcgagt atggtgtctt
     1861 gagcacttgc ataacttatt gataaacaag caattgcagt ggtaaaaagg gttgtgattg
     1921 ccttggggag ctgaatttta aacataaaac aaatatttga aattagacta taaatcgaaa
     1981 ataatgtgca aagttaaagt acacaacgtg taccactaaa taaaacatca gatgaataga
     2041 tgagaataac aagcagttag ttgtatagat ggtttcacaa taaataaatc tccaagatgg
     2101 aaaacaacaa ttgaatacct tataataatc tgaatgaaac aaaaaagagg ttgtttcaag
     2161 taagaaacaa cctctttcta aatcgaaata tgctaattat ttctgcaaca ctaattgctt
     2221 agtatattca gaaccatcaa ctaatatttt aacaatatac attcctgaag gaacattatc
     2281 atttaataaa atactttcat taatgcttcc attatttaca ctaactgaat tattgtaaac
     2341 aatttcaccc ataatattca gcaattgcat atctgcactt gttgctgttg tattaagtgt
     2401 catatcaatt acaaattcac caatagttgg gttaggataa atatcgaaag tttcagtaat
     2461 aacaccatca actaattcac cttctttaca agcagtaact gcaattgcag gtgtgttgcg
     2521 gctacaacct gaagcattgg ttacacgaca tgcataacta cctgcaacag ttgggaaata
     2581 taaataatcg ttagcacctg gaattggtga accatcgagg taccattgcc agctataacc
     2641 tccaccataa cttgctttca atttaactga tgcacataaa ctggttccgt taggagcagt
     2701 aatattcgca tttggttttg caattactga aatgccatgt gttgcggata atacactaca
     2761 tccaccggca agtgaaactt ctacctggaa gtaacctgat ttattggtac tataactaat
     2821 tgatgttgca ccaggaatta aattaccgtt tttataccat tgatacatat atcccggtcc
     2881 tgcatttgca gttaattcaa taaaatcacc tttacataaa acggtaggac ctgcaggcga
     2941 aactgatgca gtaactaaat cttcatcagt tgaaccatcg caatcattat caataccatc
     3001 acataattct gctgctccgg gatatgctgt attgttagca tcattacaat cggtattatc
     3061 taatacataa ggtgctgccg gaacataaca gaatgttgtt ccaccatcta attgatcacc
     3121 ataagtatca ccatcgttat ctaaataata agttgtaaat aatgcatcat catcaatatt
     3181 gccatcgcaa tcgttatcaa taccattaca ttcgtcgata acaccaggat gtacatctgc
     3241 atcagcatca tcacaatctt cactattttc tacccacata acaccaaaag gaccacatgc
     3301 ttctaatgta gcgcccgggt taccataagt atcaccatca gcatctaaat aaaatgtgaa
     3361 aattggcaat ccttcatcag caacaccgct acagttgtca tctataaaat tacaaacatc
     3421 atcagctgca ggattaatcg cagcattaaa atcttcgcaa tctaaatcat tgtcaacata
     3481 agttgcaggt gcgccatcac atgttgttac tgtaccataa agtccggcac cataaccatc
     3541 accatcattg tctttataat aggttacaaa agttaatccg tcatctgcaa taccatcaca
     3601 atcttcgtcc attgcattac atatttctgt tgcacctgga ttaattgaca tgtcgccatc
     3661 attacaatct gtattatcta atacataagg cgctgcagga acataacaga atgttgttcc
     3721 ggcatctaat tgatcaccat aagtatcacc atcgttatct aaataataag ttgtaaataa
     3781 tgcatcatca tcgatatttc catcgcagtc gttatcgatg ccattacata aatcaattac
     3841 acccggataa acggtatcat ctgcatcatc acaatcacct gttgttaatg accaaccaga
     3901 tacaggagct tcgcaaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     4021 caatcacctg ttgttaatga ccaacctgat acaggagctt cgcaaaatcc gaatgctgca
     4081 ccttgaccat aaccatcgcc atctgcatca acataataat ctaaaaatac aacgccacca
     4141 tcatcatgaa cacctgaaca atcgtcgtca attccattac atatttcagt ttcaccggga
     4201 ttaattgcat tgtttgtatc atcgcaatca gtattatttg caacatatcc ttctgaagga
     4261 gaacatgcag tttcgtttac atcattatca ccataaccat cactatctga atcagcataa
     4321 taaacagatt gaccggttaa atcaacaaaa atattattag tacctgtaca accatttgca
     4381 tcagtaacag taactgaata agtatccgtt gttaaagagg aaatatcttg agtagttgct
     4441 ccattggacc atgaataagt gtaataatta attgttggcc ctgttaaagt aacagtcaaa
     4501 tcatctactg ctaaaccatg atcagaggaa gtattatctg gatcattcca acgtaaccaa
     4561 atcgtttcac ccggtaaaat tgataccgaa aacgtttgat taaagctaat tctatttgca
     4621 ggtaaattcc cgtctaaggc tcctgctgtg ccgcatgaaa ctacaggacc agtaaagtct
     4681 aatgaattaa catcagtcca agtgccggtt gttatagaag ctgcattttt ttgatatgca
     4741 aaatctaaag tatttgcaac agctcctgat ccaccacatc tccattgttc tccagtataa
     4801 gaaaccgtaa ctgaagacac tgtttcgcca gtggtattgg taatttgaat tgcatataac
     4861 acaggattag cagagcctga gcctaatgaa ccaagtgctc ggtcagcagg aactgccgct
     4921 gaaccatagc tatgtaatcc acccgttgtg ttagtaccag tgtttgggaa ataaacaatc
     4981 ctatttgaat accaattagt taatgtagaa ttatctgacc atgaaggact accagtactt
     5041 gctaatgtat taaagttttg ggtatatgtt tgagttattg tggtagtaaa aggagttcca
     5101 ccgctcactg ttaaatcgat acttccatta ttacttccac caacacaagt tgcatcaaca
     5161 tgcgattcgt ttacggtaat tacagggcaa tttacagtaa atgttgcctg ataattggca
     5221 ccaccattac ttgagtaatg tgttcctgtt ccgcagcctg cgtagtcggc agtgctataa
     5281 acttctaaag tataactacc ggcaggaagg cctgtaagca cgttagttga gccggaaagt
     5341 gtttgccatt tttggtcgtt gccaccacaa cctgttccga ggttgctgtt aaagcttaga
     5401 ccaacatttg taaaagagcc tgaagggctt cccgaccaca cgcggtacca caaattacca
     5461 ttggtaatat cgcatggtga acatttaaat gttttgtttt ctccaccggc aattaccagt
     5521 gaatttccgg cacctattgt aaaggtaccc aggttagcgc cctgtaggtc ggtgtttcct
     5581 gtaatggcct gcatatcgta gaaagtattg cctgctccgt taatattgag tactgcataa
     5641 ctttcataaa taccactttg gccaaatacg ttgctgctaa caaatagcat caacaccaca
     5701 agcgttcgta aacctgcttt gagtgtaaat tttaggttca tataatattt agttaataag
     5761 tgaaatggaa gacaaatttg aggaaaaagg cagattattt ataggtatgt ggaaaaaaac
     5821 tctgctagga gcaactatta gaatttgccc ttaagccggc gattgaatca aataagggtg
     5881 gtaattgatg tgtacacagc ctttcagcca aaaagtgaga gttgtattca gattttttcc
     5941 attccattta atagattttt aacaaatatt tagcttgaaa ctcacaatta agtaggtttt
     6001 gtgcctgtta gtcatttcta tttaacacga ccctttttgt aaaacagcaa aaaagatgca
     6061 taaaccgtgc gccaaaagcg gcattcctta cctaaaaaca ttacctttgt aatgacaaca
     6121 caccaacaac agcgacagta aattatgcca attatttttc agcttttaaa aacgctgagc
     6181 ccatctgaaa ctgaccaggt taatgccttg ccatttcagg atcgtgaacg ggacgtattg
     6241 caggtaatgc tgcaaatgaa caacaggatt tttccatcat ctactgtttg taaaaggtta
     6301 ggtataacta cttcacatct ggataaaatc aactcaattt tattcaaaaa agtgattgaa
     6361 gccgtagcag gaaataatat ttaccatcaa attaattatc tcgatcaaaa aaacggattg
     6421 tggaaggctg ctttgctcat attaaaacat catgaacaca aagtaatagc attatctacc
     6481 gacaaaaccg aaaagttcga attttataag ttttattttg agtggttatt gtttaatact
     6541 gcagtacaaa atattgacga ggaaattaat cgtttaaatg acgaaatcct caataattgt
     6601 cctcgttcta cttataacga aacacaactg tggctgaaaa tttccatttt taggaaagaa
     6661 attaatatta gcacaacaaa ggcagatttt actgaccctg aaaaacaaaa agctttattt
     6721 cttaaactca ataatttaat tagtgaagct caagctatag attcagccca ttgtttatat
     6781 aaagcaaaat tatgcggtgt atttttaaat aatttattac acaatttttc tcaaagcaga
     6841 atttatatca gtaaaataaa cgaccttttt cttggttaca aggaggcctt tacggaaact
     6901 gaaatcctaa ctgccaaatg gcattacgca caaattttat ttttcagcag cgaatttgaa
     6961 gaggctttta atatttacca cagtctatac ggacgtatta ataaaaatga accattgcgg
     7021 tggtacatct atattgctga atattttcaa atttgtctgg tagttgaacg atacgatcta
     7081 gcagaagaaa tatgtgataa ttattttgct aaattttata aagacagcaa tggcaatttc
     7141 tttttatctg ccctcattca gtgtgtgaaa ttattgatcc acacgagcaa atttgatgag
     7201 gcgaaatcaa aaatcgatga attacatcgc ttaaccacaa aaacatcttc tttacagttt
     7261 caatttgctt tacgagaatt aactgctgct tatcattact taaccaaaga ttataaaaca
     7321 gcattaaccc ttgcagaaaa gaatttgaaa tttatgcgtt ctaaaaaaat gcatgtgctt
     7381 attcctgaat atacttatca tagtcgcctg ataaaagtta ttattaaaaa caaacaaaaa
     7441 aacaggcaat ttgaccctga agaacaagcg atgtttgacg aaatgcaaaa aagcacgttg
     7501 gcgcaatacg gccacttatt aaaacggctg atagcaatat aagacataaa aaaaacgact
     7561 gcaatttaat tacagtcgtt ttttttaact gttatcaatt taatttatta ttccacaacc
     7621 atatttgttg taaatatttc accatttata ttcactgcaa caacgtaagc gccattagac
     7681 aaattattta cattcaaatt ggcatttaac gctccgtttg taacagcatg atgttcgcta
     7741 tacattactt ctcctaatac atttaatatc ataatagttg ctgatgtatt aatggaagaa
     7801 gtagttacat caatagtaac aacatttgaa accggattag gatagataac caatttttca
     7861 ttttggattt ccatagcaac atcttcacct tgtttacaaa cattaataac tgtcaccata
     7921 ttggttaatc ttgagcaatc aaattcattt gtaaccaaac aagtataatt tccagtagtt
     7981 gcaggtaaat aaataaaatc ggttgcgccc ggaatttcaa ttgcatcgag ataccattgg
     8041 taagtatatg caacaccata actcgctttc aattttacgg tgcccaaaca taagtttaat
     8101 ccatttggtg tagtaatatt agcattagga tttggagcaa tatttacttc tacaaattca
     8161 cttaaaaccg ggcaagctcc cggtgcggaa acttctacct gataataggc aggtttacta
     8221 gtagtatagt taacatctgt tgcacctgtt atcaggttgc catttttgaa ccattgataa
     8281 gtatagccat ctcccgaatt tgcagttaaa gtagtgccaa catttttaca tgttgtaaca
     8341 actcctgcag gactaatact tgcagtaact gtaatttcat caatattgcc atcacagtta
     8401 tcatcaacac cattacaaat ttcattcgct gcagggttaa tggcaacgtt actatcatca
     8461 caatctaaat tatcgagcga atatccggcg cccgggaaag tgcaataatt agctgccaat
     8521 ccggcgccat aagtatcgcc atctaaatcg tcgtaaactg ctaaataggt tataccgtca
     8581 tcagtaattg tattacagtc gtcatcaata aaattacata tttctgttgc tcccggataa
     8641 atggttgcgt cattatcatt acaatctact gtagataaaa atgtatcgcc atcattatcg
     8701 gcatctacat taatattatc aatggcaaaa cttgggcgtg aacccgctcc ggtcatatcg
     8761 tgagttatcc aacgaatttg aatttcagct tgattttcgc aggcaacagg taatacgatt
     8821 gagcgcgctt gtaaatcttg tggggtggta acgccacttg cagtttgttg tgtggtgcca
     8881 ctttggtatt caacaccggt tatggcagta aatgcgccag tggtaccaat acgatactga
     8941 agtgttgctt catttaaacg ggtattggat gcaccatcat aagggttgcg aattaccatg
     9001 atgtcgtaac taagggtgat attgttatat cctgtagtat tcaacgccaa aaccaagcca
     9061 gggttggttg aaccggatgc cagcgttcca attttaccat tgtaattgtg cacgccgcca
     9121 gcattactcg aagcagacga attggctgtt aatgtatggt ctgcagttgg tccgccggtt
     9181 ataaccactg tagtagcagc cgagctcact ttccagcctt gtaagcccat tgggtaggtg
     9241 gttgatgcat gtgttaatcc ggaaaaatct tcaaaataag gaatagtttg tggggttggg
     9301 tttgtttggg caaaaacacc ggtagcaagg aacataacac ttgcagtaaa caaacttttt
     9361 agcgacaata agtaattatt tttcattggt tggtttttaa acgaagtgat aggacgtaaa
     9421 aatgaccatt atcctgcaca tctaaaataa aatagaatta acgttatgtt aaaaagtaaa
     9481 aagcctgtaa aaccctttaa attcaagcac ttcaatacac taagcgctta taattccttt
     9541 tctcgctcct gtattacaac cctaacaatt caatttgttt atacactttt cagtcagatt
     9601 ccgttattgt gaatagtatc gctttttaaa agcgctcttt agcttaaatt tgcccgtaaa
     9661 tatgtcgata atagttgatc agcttacaaa ggtatacggc gagcaaaccg cggtaaatca
     9721 aatatcattt tctgtgcaat cgggtgaaat tgtcggcttt ttggggccaa atggtgccgg
     9781 aaaaagcaca accatgaaaa tgctgacctg ttttattccc caaacctcag gaaaggccag
     9841 tgtttgcggg tttgatacag aaaaaaacag tttagatgtt cgtcgtaatg ttgggtactt
     9901 accggagcac aatcctttgt atccggaaat gtatgtaaag gaatatctcg aatttgctgc
     9961 ccgtttaaac ggcatgaaaa acgccgacaa acgtattgaa gacatgatag ccatgaccgg
    10021 gctcacctta gaacgcagaa aaaaaattgg tcagctatca aaaggatacc gtcaacgcgt
    10081 tggtttggca caagcgatgt tacacgaccc taaagtgttg attatggatg agccaacttc
    10141 cggtttggac cctaatcagt tagctgaagt gagaagtctg attaaaaata ttggcaagga
    10201 aaaaacggta attctatcta cccatattat gcaggaagtg caagctattt gcagcagggt
    10261 aattatcatc aacaagggta aaatagttgc cgacgattcg gtagatggca tacaggcgca
    10321 acaaaaaggc accactatta ttcaggtgga atttaaagaa gccgttaatc atcaattatt
    10381 aaaacaaatt gcaggtgtcc gcagcgtaaa aggcgatacc aagttggtga ttgaatatta
    10441 tggcgatgcc gatatcaggg aatatttatt ccgatttgca gtggaaaata aatacacatt
    10501 attaaacatg catatcgaaa aacaaaatct ggaagatgta ttccacgact taaccaaata
    10561 attaaatcat tagaattaaa aatattgtgt tttgatagcc atttttagaa aagaactcaa
    10621 cgtatttttc agctcgctga ttggatatat tgccattact atttttttaa tagccaatgg
    10681 tttattcgca tgggtatttc cggaaactaa tattttggat gacggatatg ccactttaga
    10741 ttcatttttc aatttcgcgc catggatttt aatgttttta attccagcaa tcaccatgcg
    10801 ttcgtttgcg gaagaaaaaa atagcggaac cattgaattt attactactc gtccgattac
    10861 cgatatgcaa attatttttg gtaagtactt agcggccctg gtgttggtta tttttgctat
    10921 tcttcctaca gtgttatatt attttacggt aagtgccttg gcatcgccag caggtaatgt
    10981 ggacaccggt ggtataattg gttcttatat tgggttgatt ttattaggcg caacatttgt
    11041 atcaattgct ttatttacat ccagtatcac caataatcag attgtagctt ttattgcggg
    11101 catgtttgct tgttttttct tttaccttgc tttcgactct ttatcgctaa ttccggcatt
    11161 tattggaact gctgataaat attttaatgc aataagtatt aaccgccatt atttaaatat
    11221 cagcaaaggt tatatcgatt tgcgcgatgt catttatttc agcagcatga ttggcatatt
    11281 tattttaatg accaaaacgg cattaggaag cagaaaatgg taattcatta attattgatt
    11341 aagtgtaaaa aatggagaga caagaccaat ataaaaaaaa caggagaaga gcaatgttac
    11401 aattgctatt acttgttggt attattgtaa ttattaatgt tttactttca ggcttctttt
    11461 tcagagttga tttaacagaa gaaaaacgtt tttctatcag cgaaccaacc aaaacacttt
    11521 taaaagaaca ggaagatata ttatttattc gcgtttacct ggcgggagat ttaacaccaa
    11581 acctaaagcg actccagcaa gcaactactg aaatgttgga ccagtttaaa aattatgccg
    11641 gcgataatgt gcagtatgat attttcgacc cattcagtat cccgaatgaa gaaaaccagg
    11701 cagaattcat caaacaactg gaagaaaaag gcattttaag tgttcaattt atggagagtg
    11761 caacagacga agcttccata aaatatattt tcccatttat ttccatcaat tataaagatt
    11821 cggaaattgc atttccaatg attgaccgag gcaccatgcc attgccgcta aatccggata
    11881 gtgacccttc ggtttcgatt tcattgttag aatataattt cactaaagcg atccgtcaaa
    11941 ttaccgatgc cgacaaacct tatgtggcat ttattgcagg ccatggcgaa ttaaatcgtt
    12001 ttgaattaaa tgatattgca ggttctttaa atgagttgta caatgtcact cagatagatt
    12061 tggaagacga cagcacttat gaaattccta aagaaataaa aacaattata gttgcaaagc
    12121 caatgtatga gttttctatg gaaggcaaat ttattatcga ccaatatatc atgcaaggtg
    12181 gcaacgtgct ttggttggta gatcctgtaa ttgccgattt cgatagtttg tattccggta
    12241 aagggcaatt tatggcaatt gatcgtgaat taaatattat ggatatgttg ctgcaatatg
    12301 gtgcacgtat aaataccaat attattcagg acaaacaatg cacgcatatt aatgttcctg
    12361 ttgccaatgg cgaaaatttt atcatgcgcc catggccgtt taatcctatc ctcaataatt
    12421 ttaatggtga tcatcctatt agtaaaaacg tagatgccat agaaggaaaa tttgtcagca
    12481 ctgtcgacac cattgctgtg ccgggtataa ccaaaaccat attattaacc acatcaggat
    12541 taagcagata tttggtagca ccggcaaggg ttaattttaa tgttgtggat acaaaatttg
    12601 caccaacgga cgagcagtat aacaaaccta atcaaccggt tgcagtttta ttagaaggtc
    12661 agtttccttc agcatttaag tctttaaaac caacagcaaa tcgtttacat caaatgggct
    12721 ttgcatatca ggacggaaaa gttttggaaa aaggaacagg ctcaaaacaa attatgattg
    12781 gcgatggcga tttaattaaa aactttgttg atgaaaacgg caagcccgat atgttgggca
    12841 ttaattatat cgaaagatat atttatggca ataaagattt tgcaatgaat tgtatcgaat
    12901 atttatgcga ccaaaatgga ttaattgcta ctcgcgcaaa agaagtaaaa ttgcgaccgc
    12961 ttgatgaaca aaaagtggga gaaaacagaa ttcaatggca attggtgaac atgattgctc
    13021 cattattggt tttatatctt tttagcggaa tatatttctt tgtccgcaac agaaaatacg
    13081 cagcttaaat tgtaaaaatt atgaaccgca attggttata tttcggcatt ttagtgattt
    13141 tggcagtaat tgcttatttc acattaatta gagacaacga aggttcctac agtaaaaaag
    13201 atactgcatt tgctgttgaa gacactaccg caattggcaa aatattgtta accaatttaa
    13261 agggcgacag cattttatta gagcaaaaca atggcagttg gacgatgaac aaagactacc
    13321 ggcctcgtcc tgatgcaatt cataacttgt taaatacgat ggttcaactt gaagtaaaaa
    13381 tgcctgttgc caaatcgatg cacaataccg ttgttaaaaa aatagccggc aaaagaaccc
    13441 atgttgaaat ttttaacagc aaaggagaaa aaacaaaagg attttatatt ggcgacaata
    13501 ccgatcaatt gaatggtaca ttcatgttga tggaaaactc ggaccatgca tttgtggtga
    13561 acatccccgg ttttgcagga tttgcagcta ccgtattttt taccgatgaa acagattgga
    13621 aatcaaaaaa tatttttgct tataaacccg atgctattaa tcagatagat atcacttata
    13681 acaacttacg cgattcgtca ttcagtattg ttcgcaaagc agataacacc tttgaattaa
    13741 taggcaataa tactattaac aaaccattga atcctgaaat tgtcagctat tacttaaaac
    13801 aattcggcat gttgaatgca gagtatttta ttttagaacc tgaaaaaaga gattctttgc
    13861 tggcaacaac tccggcgtgt attatgagtg ttactgataa caataaaatc actactacat
    13921 taaaaatata ttatcgtccg ctcacttacc gctcaaaaat gcaatacacc cttgatgaca
    13981 aacctatcga atttgattta gataagtatt acggtgtatt taatgaggat aaagatttgg
    14041 ggattataca gaattttgta tttggcaaat tgatggttgg gcctcaattc ttttatcgcc
    14101 agcggccggg aaatgtgagt gtattgaacg aaggattaaa ataaaaaaaa gacctgccag
    14161 gtttttgaaa ccaggcaggt ggtttttaca attttatgag ttgtttagta agcacattat
    14221 cttcaccaaa catttttacg atatataatc cgggagcgaa aacgcttaca tctattgtta
    14281 ttgaacttcc cgccatacat tttgcctgca acaatggcat tgtttctcct aactgattaa
    14341 taatttgaat ttgttcaatg ttttttaatg agctgttaga gataataatt tcaccactta
    14401 ccggattcgg gtaaatgctg atatcattaa ttgaaacatt ttgaattgca acaggcgccg
    14461 tataatttat ccatacttta aataatctgt tactggcagt gctgggagta aaattcggga
    14521 tgagtgcatc tataccacca taaatatatc ctgccaaaac actcccgtta atatcgtgta
    14581 atctaattac gcggttatta taatgcggaa ttgcattatt taaaataaat tcggcattgg
    14641 aacctaataa tgcatcaaat tgaatgggtt ggggatattg ttcggtggta ccatcggcat
    14701 attttattat ggtggagatg tcatttacaa atggcacatt taaatcctgc tttatagtgt
    14761 catcttcttc atcataataa tattgtccta ttccaccgaa taatgttgcg taataatttt
    14821 gtgaaacaga atcgtaaata ctcattacag gacaggtata atgatttaat ttttggtaaa
    14881 aagcttcatc ttctgaatag ccagttgttg tgacataaac aggtgaaaac caaacccaat
    14941 cgccctcata ttgaaacacg ccactaaaag ctgccaatgc ttcttctgcg ccgggtaaca
    15001 taatttgttc aaaatttaaa tcgcgtcggt gaaatactgt tgtatctttt atgatggtga
    15061 tatcttgaat agttatagtt tctccatcgt cagctaatgt aaatttttta agtgcatcgg
    15121 tgtattcctg aaaaaactgt ggacctgcgg gttttgaata ttcacccgcg aaaatatgtc
    15181 cgccaaataa ataaatggta tcaccaatta aaccaatttc gcccccgctt acttcgaata
    15241 aagtatcagt taaacttctt atggcgggtg caacagactc attggcatca attaaattac
    15301 tcatcatttc caggtcgaca gccagtaatg aagggtaggt tatcatacta tctgttgatt
    15361 gttgatgacc ataaccgcca ataatgtata aatatttacc tctttgaaaa aattgtgcgt
    15421 tggtgctttg caactggtcc tggacagctt cgggcaaagt gaacaaattt gcggactggt
    15481 aatcgccggt ttcggggtca aaaaggtgga tttgtttgtt ggcatcgatt atagggaaag
    15541 gtgttggagg caaaaaacca tgcagtccgc cggtccttcc ggtcatgatt aaccatcttc
    15601 catcccattc gccgagggca aaagagtgta ttcccggcca atcggagtaa gttacttctt
    15661 caagggtaat ggtaaatggt ggagctgtaa cctgcgacag caaaggtttt tgagcaattt
    15721 ggaaacaaaa caaaacgctc attacaaggg caatcctatt cattatacaa agttagtatg
    15781 aaatccacca tttgttgaaa acacttttag cactaatgtt tacctttgcc ttaactgaaa
    15841 attatgagca tgaagtttat tgtaagtaca actgcgttat tgcgcaatct ccaaacggtg
    15901 aatggcgttg tgagcccgaa tacggtttta cctattctgg aagatttttt atttgtggtg
    15961 gaaaagaaca agctcaccat ttcgggaacc gacctggaaa catcaataac cacatggatg
    16021 gatattgaat caaaatcaga aggtaaagta gctatcccgg ctcgtattct tgttgattat
    16081 ttaaaaacgc ttcctgaaca accacttact ttttctatag atgaagatag ttgccaggta
    16141 gaaattacta ccgaccgcgg taaatataaa ctcaacggtg aaaacggaga tgattttccg
    16201 cgtgtatcga gcgatgataa aatgagtgat attactttac cgtcaggtgt tttacaaaaa
    16261 gtaattaata aaacagcatt tgcaacaggc accgatgaat tacgtcctcc tatgacaggt
    16321 gtattgtttc agttaaataa agatggtatt aatttcgtag caactgatgc acataaatta
    16381 gtacgttatc gcagaacaga tgctaaagca agcggctctg catctttaat tatgcctaaa
    16441 aaagcattaa acttattgaa atctaattta ccgggtgaac aaactcaggt aactatttct
    16501 tataacagtt catttgcgtt ttttagttat aacaacgtaa aattaatttg tcgtttaatt
    16561 gaagggcgtt atccggatta caatgcagtt attccaactg aaaatccaaa tcgtttaacc
    16621 attaaccgtg ctgatttctt aaatacttta cgtcgtgtaa ttatcttctc caataaaact
    16681 acccatcagg taattttaaa aataaaaggg aatgagttaa ttgtaaatgc acaagattta
    16741 gatttcagta atgaagctaa tgagcgttta gactgcactt atgaaggcga taacatggaa
    16801 attggattta atgccaaatt cttattagag atgttgaatg cgatggacaa cgaagaagtg
    16861 cgtatggaac tcagcacacc aaacagagcc ggattattat taccaaccga aagtgatcct
    16921 gcagaggatt tattgatgct ggttatgccg gtgatgatga atgtttaatt ttcaatttca
    16981 aaatattaaa aaagcgatgt aggtttctgc atcgcttttt tatttcacgc ggagggcgga
    17041 gttgcggaga aataaactat aaaatttatt tatacctaca aattaaacgg ttgtcgtgga
    17101 gtctccagac cgattgcgtg ttcctacgaa tagtaatttt caaatttgtg tcacaaatgt
    17161 agatttaagt taaatgcaca ctcaaaccaa ttcatctttc gtttaataaa aatccttttt
    17221 cattatccca gaagcaagtc aaaaaccttg tgaaagctgg agattggaat aatttatatt
    17281 tgattgaatg atattataaa ctactctttc cttttagtat tttctttaac cgggaggtca
    17341 ccagttggtt gattaaccaa tgtactttgt ggttgtttaa ttgtaagtga taaaattccc
    17401 attccaaaag catcggtttc tacatcacca attactatcg aaacctgaac atatccctga
    17461 atagtgccat cgcttaattt acgcacatca acaaattgaa tacccaaatc attatcattg
    17521 aaccctgatt ccgttactga tacaattctt tttccatcaa ccaccaaacc gtgcgtgcgt
    17581 ccgttatcac aatctgcaaa ttgatcagga aatttatctg taaccatatc ttctttcaag
    17641 tctaaagaaa tatccatagt aagcggtcca agtgtatgtg ctttaatttt atttggctta
    17701 taccggaagg caattttata ttgcccatgc aatggagacc caatccaatc aacaaaacga
    17761 tcagaagcat cctggacaaa aaacaaccaa tatccctcgc catttttggc atttactgtt
    17821 ttatttctaa accaaaattg ctgcgcagga tgtacttcag gcacattacc atgatgcctg
    17881 tcggtagtta cagcaccata cataccaaat gtatctaatt tatgcaaata gataccactt
    17941 tcacagtacg tattatcggc tcgtctaaaa aagaaattat tatcaataaa actattaggt
    18001 actgcaattt ctcctaataa aaacgggaca tttttatcat aatcaccaca agttaattca
    18061 ggcccattta aagttgtagg gaataaccag tcatcatttt tattaaatgc tacaatatca
    18121 taaaactgtt ggtattcctc aaacaaaatc atccacaagt taatgtcgtt cgaattatta
    18181 tcttctgcat gcgaatgata tctgtttaag gtgccgccaa tattttgctt ttcttttgac
    18241 aatgcattaa aaatcggctt ataaaatggc aaaccaccga atacatcagt agttaatgct
    18301 gtatgttgtt ccatggtgct taactcacct ttagttgggt cgtaagctcg tatacctaat
    18361 aaagtttcag tagttaattc ccatgttttt atttcttcag cggaagctaa tggaatattt
    18421 tctgatgata gagttacatc atcattatca caacgcacaa ataaacatgt ggttgtaatt
    18481 gcaattatta aatttaaaaa tgttttcata ataatggatt taaaaatgtt aggtagatat
    18541 aaaataggat aactaattaa ccaaagttag gttgttgttt tgatttatta tcatgatgta
    18601 agcaaattca cggtttaccg ttaatcatgt ttttgtttaa aaatatgaat tatactaagc
    18661 attaggttat tgatatgtac accggatgca aatgcatgcc aatcgccaaa ccaagctgca
    18721 ggttcgctta ataaagggca aattgttatt aaaatgtcaa aatttacttt aatatttgtt
    18781 tttttgtaac atgatggata aatttggtta ttccaatcac cctaagacct gttcatgaaa
    18841 agcaaattga ctaccccctt tatcgcaatc ggaactattt tattgatttc aagcagctgt
    18901 gaaaaaacaa ctgaagtgga tttaaatgcc gaatatttag gtaaatatcg tatagaatat
    18961 acttatgagg agccttattt taatggcact gattgggaaa ataatacgat taccaatatt
    19021 gatagcacct gtgaattaac gaaagacgca aatggggatt tattatttac caacttcgat
    19081 tttttttggg acttaccgat gaaaacttat atagaaagtg atagtatttg tataccgcaa
    19141 tatgattact atttaggagg gccttatcca aatacttata aggcttatgg taatgtagtt
    19201 gatggggaat tagttttgga aataaaccgc actctttatg actggacttt catgatctca
    19261 cttaaccagc ctgggacagc tgttgctacc aaaatagatt aataggataa attatcgtct
    19321 tat
//
LOCUS       DAORKW010000016        18861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_26_length_18861_cov_5.150058, whole genome shotgun sequence.
ACCESSION   DAORKW010000016 DAORKW010000000
VERSION     DAORKW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18861
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26_length_18861_cov_5.150058"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PLB46_01490"
     CDS             complement(<1..1078)
                     /locus_tag="PLB46_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRF75212.1"
                     /translation="MKVIIIGGGIGGLSLAIALQRKNIPFEIYEATPVLQEAGAGIWL
                     GGNAMNALDALGVGDIIKQKSNRLGKVFIKTQSGKVLQTVDCDILEKKYGNATYSIHR
                     ATLQLTLAACINQTINTGHRLSSIRQLDDKVEAVFENGNVAVGDIIVGADGIRSLVRE
                     QYVTKANYRYSGQTCWRAIIDTRLPPNEFSVGAEVWGNKGGLRASYSQLGGNQVYFWT
                     TMKMAAGIKLLNEDALGLFQKQLTPFSGYMQQIIRMIQPERLIHSDLFDIQPIKKWYH
                     NRIVLLGDAAHATTPNLGQGASQAIEDAWVLAENLAKYDYTNAFANYQQKRIARATKV
                     VNTSYQIAQLTNLGGPVFSIIKSAL"
     gene            1145..1759
                     /locus_tag="PLB46_01495"
     CDS             1145..1759
                     /locus_tag="PLB46_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF75213.1"
                     /translation="MRTKEKILNTTLQLLNEQGFKSVTIRDVAAEMGISHGNLGYHYA
                     NMELIIYALYERLVKEMDAIIGSMQFSGIHLQLVYDSNKVMFNKFYEYRFLFIDFVAI
                     GRSIPKLRKHYLALQKKRAAEFFMLLMLLKSNNILSSKFSDEQYGQLIQSLTIFGDFW
                     ISSSELVYNGKKDKRVDYYLHLFFALIQPYLTKEGQKQYLRLNN"
     gene            complement(1767..2999)
                     /locus_tag="PLB46_01500"
     CDS             complement(1767..2999)
                     /locus_tag="PLB46_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRF75214.1"
                     /translation="MEQPSLLPDLFRTEYSKIVAVLCKYFGMPFLEQAEDIASETFLL
                     ASNTWGLKGLPNNPTAWLYTVAKNKAIDFIKHNQVFKQKVYPEILVASPTAYVPEPDL
                     SPSNIMDSQLKMMFAICNPIIPVEAQVGLALNILCGFGADEIAEAFLTNRDVIYKRLQ
                     RAKQKLRDENISIDFPVQNEIPLRLSAVLTTIYLLFNEGYYSASQNKTLRKDLCLEAM
                     RLNVLLLEHQNTNTAESNALLALMCFHASRFEARSNNNGEIVLYEEQDEQLWNKPLIQ
                     KGEYYLNHSAKGSHISTYHIEAAIAYWHTHKQDTVEKWENILQLYNKLLQIQYTPITA
                     LNRTFALSKVNGVDEGILDAEKLKLINNKMYHVLLGKLYNEVNQMQANYHWEFAIKLS
                     KSDTEKAYFKSVINKLSK"
     gene            complement(3050..3397)
                     /locus_tag="PLB46_01505"
     CDS             complement(3050..3397)
                     /locus_tag="PLB46_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRF75215.1"
                     /translation="MNDFLLVFRNDVALSQTQMSPDQMQTMMKTWMDWIGGIAAQNKL
                     VSPGNRLAPSGKVLATNNVITDGPFVEIKEAIGGYIIVKAETIDEATQLSHGCPILDV
                     GGTVEIRMIIKMD"
     gene            complement(3419..3802)
                     /locus_tag="PLB46_01510"
     CDS             complement(3419..3802)
                     /locus_tag="PLB46_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF75216.1"
                     /translation="MNANVNALNWFEISVTDIARAKKFYESIFGIQMHQEEMMGMQMA
                     YFPYEDMNGKVSGALVQSEFHKPSADGAKIYLNGNPDLENVLSKVEAAGGQITMPKTF
                     IADNVGHMAFFIDTEGNSVALHSNN"
     gene            complement(3914..4321)
                     /locus_tag="PLB46_01515"
     CDS             complement(3914..4321)
                     /locus_tag="PLB46_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75217.1"
                     /translation="MKNKIGTKDKPLTLKTPPLSSEYTMHVDEKDGKEILVCTVGKTI
                     LHYDIRCLNDLHQMLKKHGDWMELGSADEQKPAKEGTVEAWGRSEKNPVGGWYGLKKG
                     FRGRFGMYIPPLMEALGLAEVTHDAKGNKMRAK"
     gene            complement(4349..4858)
                     /locus_tag="PLB46_01520"
     CDS             complement(4349..4858)
                     /locus_tag="PLB46_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF75218.1"
                     /translation="MKQFITILTFMTVANISMAQNGTATTTKTTFSRETTVSINIQAN
                     QAIVWSLLTNASDFPRWNSTIVSIDGNIALGEKIQLKSTLDTTRTFKLKVKSLEKEST
                     LIWGDGKGDRTYTITPNETGGVLFTMTEKIGGLMFPMYAKYIPSFDASFEQFAADLKK
                     EAEIIHQTK"
     gene            complement(4906..5709)
                     /locus_tag="PLB46_01525"
     CDS             complement(4906..5709)
                     /locus_tag="PLB46_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF75219.1"
                     /translation="MQTTQLTNEFLQHKGQLKSFLLRLTASVEDAEDIMQDTYIKASE
                     KIESFRAESSLKTWIFTIAANLAKDNLRAKRRWTEDVTDRGKAAALTDKNFFAEAMQI
                     RMTSPHGAFEIKEHITFCFTCIAKSLPLEQQICILLKEVYEFKVNEITEIVNTTEAMV
                     KYYLHTGRAKLVQIFEGRCALINKEGICHQCTELNGIFNPKQNHQEELNKIEMAKQAN
                     NPDKEHLFDLRMQIVQGIDPFESPSAELQLHHYAHNSRVMENFLKKNEV"
     gene            complement(5786..7123)
                     /locus_tag="PLB46_01530"
     CDS             complement(5786..7123)
                     /locus_tag="PLB46_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75220.1"
                     /translation="MQSIIKYISLITLLIFIHYKLSAQINPDNNIISLDISTSPQNPA
                     FTYDSCFQKGVDAGIGSVGLYQNWTMIEVAPLTYNLSLFDIANLYYPAYNMPLDLTIT
                     PIHTNNLEVPADLVATPYNNPTFISRFKAMLDSVHAHIPDVTLNSLVIGSEYDVYLGS
                     NTTLWNNYAEFYNAIVIHAKTLWPDTKIATEITFGGLIVYNTNAQIVNTNSDYIGVSY
                     YPLNPDFTVKPVTAIADDIEALIDLYPDKPLFFYQYGYPSSTLCNSSDFLQRDFITKT
                     FEIWDMHPTAIKGFDFTWLHDLDPVMVDYWGDYYGLTDEVFLEYLRTIGLRTYEGMGT
                     DKPAMQELLCQAALHGFSDVDVECITAISDDNLLTTLTIYPNPVKDYFSINGLPDNAT
                     TITIYNMFGEIVSVISTPKNGMIDVNHLTNGAYAVTIKDEAKTIAIDKFVVLK"
     gene            complement(7166..7522)
                     /locus_tag="PLB46_01535"
     CDS             complement(7166..7522)
                     /locus_tag="PLB46_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRF75221.1"
                     /translation="MSTNTIQYYMPGNRLARATVYNNTIYLSGIIADADAIDFKSQME
                     SVLQKMENTLSDLGSNKNKLLQVTIYLPDITQFDYMNVIWDKWISPGLAPARATVEAK
                     LAHPKYLIEIMATAAI"
     gene            7694..9340
                     /locus_tag="PLB46_01540"
     CDS             7694..9340
                     /locus_tag="PLB46_01540"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRF75222.1"
                     /translation="MKNDQARYIFVTGGVTSSLGKGIFSASLAKLLQARGFRVTIQKF
                     DPYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLGIETSQANNVTTGAVYQNV
                     INRERAGEFLGKTVQVIPHITDEIQHRMLLLGESGEFDIVITEIGGTVGDIESLPYIE
                     AVRQLKWKLGEKRCIIIHLTLIPYLNAAHELKTKPTQHSVKLLLESGLQADILVCRTE
                     HPLPKELRRKIALFCNVNVNSVFQALDVKTIYDVPLHLLEENADEIVLTKLKLPKRNE
                     PDLKNWKAFLSRLKNPTNSVKIGLVGKYVELQDAYKSILESFIHAGAANECKVKVISI
                     QSEHITDSNVAEKLADLDGVLVAPGFGDRGIEGKITAIKYVRENKIPFFGICLGMQCA
                     VIEFGRNVLKLKDAHSTEMNPKTKHPVIHMMEEQLEVTEKGGTMRLGSYDCRLFKKTK
                     AYEIYKSQHITERHRHRYEFNNVYLKDYEAKGMMATGLNPQQNLVEIVELKDHPWFVA
                     VQFHPELKSKVENPHPLFVGFVKAAIDFKEKKQDSVTAEQ"
     gene            9488..11410
                     /gene="yidC"
                     /locus_tag="PLB46_01545"
     CDS             9488..11410
                     /gene="yidC"
                     /locus_tag="PLB46_01545"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRF75223.1"
                     /translation="MDRNNVIGFVLLGVLIIAFFFMQSRFANQQRVAEAQKQATEDSI
                     KRVEDSLNLLKNPILEEKKETTLSNAAVAIPDSLISTLSADAIAKMEDSIQQLQKKNV
                     FGRFSAVAQGTEKLDSIENDLIKVVFTNKGGHIVKAELKKFKNYHGGPVDLISGNDNR
                     FNYNFKYNNDKMINSEELYFTASKSSDGKSIKFIADLGEGSVFEQTYAFTATDYLIDY
                     NVNFKGFENIISRQDPIIILNWKNVMQEQEKALKYERQYSKLYFSDVDGDVDYKNTNG
                     ELKFETPVKWVSCQQQFFNTTLIAKESFDRKGKIEVFADDTSAFVKSCNTELYLTYDG
                     KKDFTVPMQWYIAPNDYQSLNSMDLGMETIIPTGSGFIGWINTHAIIPLFNWLSGMIT
                     NYGLIILLMTLIIKLVLTPLTYKSYLSMAKMRVLNPELTEIREKYKDDQARLGQEQLK
                     LYRKAGVNPMGGCIPTLLSMPILIAMFRLFPGTIDLRQQSFLWADDLSTYDDIISWGT
                     SIPLIGNHISIFCILMTLSSVLYIRMNMQNTAQLQGPMKTVQYLTPVFFFFFLNSSPA
                     ALTYYYFVSNIVTFGQQWAIRRFFINDDDIHKKIQENKAKPEKQKSGFAKRLEDAMKQ
                     QQKMREVQQNQPKKKK"
     gene            11513..12556
                     /locus_tag="PLB46_01550"
     CDS             11513..12556
                     /locus_tag="PLB46_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRF75224.1"
                     /translation="MPLSLSSLKQRNNVVLLVTIFLGAIIAFGLNRILGGLLGAIIIY
                     VLFRPLNIYLQEKKNWNKSLSTSIILVLSFFCLIVPIFLLVTLITDKALYYVNHPELT
                     DQMFGNLNKFAEEKFNEPDLVGNLLETLKAGAGTFVSTVVNGAANTFIQIIVMYFTLF
                     FTIKNFREFEKGLTQYLPFRRANSIRIGNELRNMTYSNILGQGCIAIIQGALLGLGFW
                     IFGIPDPFFWGVIGAFLSMIPLFGSPLIFLPAGIIEMSNGNMVSGIGIIVYGYLIVTM
                     VDNFIRMAIGKRIANTHPLITIIGVVIGIPIFGILGILYGPLIISLFIILAKIYRENR
                     NEIIQLSGEDDNT"
     gene            12567..12926
                     /locus_tag="PLB46_01555"
     CDS             12567..12926
                     /locus_tag="PLB46_01555"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRF75225.1"
                     /translation="MEKSNNPNLSENLNEAIDDVKEYVNLRMQLIQLNVTEKVSIGLA
                     GLIAGGIALILFVLCFIFGSFALAYLLGNVLHNTAAGFGVMAGFYLLLGIIMLKVGKG
                     KLRITLINSFIKQFSND"
     gene            12936..13379
                     /locus_tag="PLB46_01560"
     CDS             12936..13379
                     /locus_tag="PLB46_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75226.1"
                     /translation="MEPITINSVEDLQRAKAQLRVQINNKEQDLKAHYKTISNQVAPA
                     LRIANVVSGNRLFKSALGGTDENDNGWLSSAMKILTAVSAGGFILHQNKKNLGKALLA
                     YALDQGVKYIKEHDLSEHIDKLKKWLNLETEQTVNNTQDNDSEAE"
     gene            complement(13385..14719)
                     /locus_tag="PLB46_01565"
     CDS             complement(13385..14719)
                     /locus_tag="PLB46_01565"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRF75227.1"
                     /translation="MKTIIKNTRIVNEGKIAEGDVLINENRILQIGGIIQTNGNYTEI
                     DGEGKYLMPGIIDDQVHFREPGLTHKADLFTESRACVAGGVTSFMDMPNVNPPSLTQE
                     LLEQRYQLAATKSLANYSFYMGVSNDNAEEVLQTNPTNVCGIKIFMGSSTGNMLVDNR
                     ATLEHIFKNAPVLIATHCEDESTILRNLDKAKEQYGENIPVHMHPIIRNEEACYLSSS
                     FAIELAKKCNTRLHILHISTAEELALFSNAVALSEKKITAEVCVHHLFFDADDYAQLG
                     AKIKWNPAVKDKRHKPELLKALLNGTLDVVATDHAPHTLEEKANLYSKCPGGAPMVQH
                     SLMLMLDFFHKGQITLPQIAEKMSHNVATCFQIKDRGFIREGYFGDVFLLDADKPQLI
                     TANSLHYKCGWSPLEGYTLKGKIEKTFVNGNLVYDNDKFVEGTNGARLLFNR"
     gene            complement(14750..15850)
                     /locus_tag="PLB46_01570"
     CDS             complement(14750..15850)
                     /locus_tag="PLB46_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75228.1"
                     /translation="MIKITHLVLIAAITTGGIAGCKQSSKVEKPQEETTVVVDTITYP
                     QEKHLKNMRQLTFGGDNAEAYFSFNSEMIVFQRRDNKEVPCDQIYYGKIPTDNSRFEY
                     KLLSTGTGRTTCSYFLPGNDKVIFASTHAQVDTCIADPDKSKGYLWGVYPSYEIYVAD
                     LKGNIVKQLTDNNFYDAEAVVSPKGDRILFTSDRSGDLELWTMNLDGGDLKQITFDLG
                     YDGGAFFSPEGDRIVFRASRPKTPEDIATYKSLLKDGFVKPTAMELFTCKADGSDLRQ
                     ITQLGGANWAPFFHPSGNKIIFSSNHTTKRVPFNLFMINLDGTGLEQITFDTVFDSFP
                     MFSYDGKKLIFASNRNTGGNRDTNLFIADWVE"
     gene            15943..16998
                     /locus_tag="PLB46_01575"
     CDS             15943..16998
                     /locus_tag="PLB46_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF75229.1"
                     /translation="MISKKGLVIGIIGAAVITAGILLAKSNVFKGGNKNEVAVDSLEM
                     AEQLTPQIADLSLQINGNPQNAGLYYARANEYFNFGNLKFALEDYKKAFLLDSTNATL
                     ALGLSDCLFELNNADGAIGILKDYLKTDPENIDIMLNLAIDYYLLPKPQYQKAIEVLN
                     DVLKVDVQNSEAYFYKGMIYKETTDTTKAISNFQTAVEADPDYYDAYMQLGLLYAEQN
                     NDIAIKYFDNAIALNDGSNEAHYAKAKYYQDRGRISEAIGFYKKMIVADPQDANAIYN
                     LATIYFGIDSIQQAYRFYELAIKQAPAKAMGYYGKGLCAEELKNKEEAISLYNQALNL
                     DPDLKVAEERLLILNEK"
     gene            17002..>18861
                     /gene="thrS"
                     /locus_tag="PLB46_01580"
     CDS             17002..>18861
                     /gene="thrS"
                     /locus_tag="PLB46_01580"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRF75230.1"
                     /translation="MIKITFPDGNIREYQEGVTSMEVAKSISEGLARKILSAEVNGEV
                     WDLSRPITSDATVKLLTWDDPKGKNTFWHSSAHLMAEALEALYPGVKLGIGPAIDHGF
                     YYDIDLGGRVLTVEELPKIESKMKELAKTNSAYIRKEVSKADAIEYFTQKGDEYKLEL
                     ISDLEDGSITFYSQGNFVDLCRGPHIPETGVIKAVKLLNIAGAYWRGDEKNKMLTRLY
                     GITFPKQSELDEYMVMLEEAKKRDHRKLGKELEIYTTDDEVGQGLILWMPNGAAIIEQ
                     LEKLAKRKEEQAGYVRVKTPHIAKEDLYLTSGHLPYYADSMYPPMEMEGVKYYLKAMN
                     CPHHHKIFSAAPRSYRDLPLRFAEYGMCYRYEESGALFGLMRVRGLNMNDAHIYCTEE
                     QFENEFKAVNDMYLNYFRIFGIDKYVMRFSTHEPKKLGQKYLDNPELWKKTEDMVRRV
                     MDNAGIPYIEVPDEAAFYGPKIDIQIYSAIGREFTLATNQVDFGQPLRFGLTYTDRDN
                     QPKMPLVIHRAPLGTHERFIGFLLEHYAGNFPVWLAPVQVAILPISDKYNEYCQEVIN
                     VLKNNEIRTYFDTRSEKIGRKIRDAEVKKIPLMLIVGEQEMNSKTLSLRKHGEG"
BASE COUNT         5917 a         3387 c         3521 g         6036 t
ORIGIN      
        1 ggagtgcgga tttaattata ctgaagaccg ggccaccgag gttggtgagt tgggctatct
       61 gatatgaggt atttaccact tttgttgcac gagcaatacg tttttgttga taattggcaa
      121 atgcatttgt ataatcatat ttagctaagt tttcagctaa tacccatgca tcttcaattg
      181 cctgactggc cccctgtcct aaatttggtg tagtagcatg tgcagcatca cctaacaaaa
      241 caattcggtt atgataccat tttttaattg gctgaatatc gaataaatcg gagtgtatta
      301 aacgttcggg ctgaatcatt cgaattattt gttgcatgta tccactaaac ggggttagtt
      361 gtttttgaaa aagtcctagt gcatcttcat ttaataattt aatgccggca gccattttca
      421 tggtagtcca aaaatacacc tgattgccac ctaactgtga gtaagaagcg cgcaatccgc
      481 ccttatttcc ccagacctct gctcccactg aaaattcatt cggtggtaac cgtgtatcaa
      541 taatcgccct ccagcaagtt tgccccgaat aacgataatt cgctttggtc acatattgtt
      601 ccctcactag tgacctaata ccatctgcac caacaattat atcacctaca gccacatttc
      661 cattttcaaa aacagcttcc accttatcgt ccaattgccg aatgcttgat aagcgatgtc
      721 cggtgttgat ggtttggttg atacatgccg ccaatgtaag ttgtaaagtt gcccgatgaa
      781 tactataagt agcattgcca tatttttttt ccagtatatc acaatctaca gtctgcaata
      841 ctttgcctga ctgagtttta ataaaaacct tacctaggcg gttacttttt tgttttatga
      901 tatcgccaac gcctaaggca tccaatgcat tcatggcatt tccacccaac caaatgccgg
      961 ctcctgcttc ctgcaaaaca ggtgttgctt catatatttc aaaaggaatg tttttgcgct
     1021 gaagcgcgat agccagtgac aatccaccaa taccaccgcc aatgataata actttcatag
     1081 ggcaaattta ggacgtttgt cctatttttt agtacatttg ccctaaatta tttacatttg
     1141 tacgatgcgc accaaagaaa agatattaaa taccactctt cagctactca atgagcaggg
     1201 ttttaaatcg gtaactattc gtgatgttgc tgctgaaatg ggtatttcgc atggtaacct
     1261 ggggtatcat tatgcgaaca tggagcttat catttatgcg ctatatgaac ggcttgtaaa
     1321 agagatggat gccataatcg gcagtatgca attttcgggc attcacttac aattggtgta
     1381 cgacagtaat aaggtcatgt tcaacaagtt ttatgaatac cggtttttgt ttatcgattt
     1441 tgtagcaatt ggcaggtcaa ttccaaaatt gcgcaaacat tatctggctt tgcagaaaaa
     1501 aagggcagct gagtttttta tgttgctgat gttgttgaag tcaaacaata tactttcatc
     1561 caaattcagc gatgaacaat atggtcagct catacaatca cttaccatat ttggcgactt
     1621 ttggatttcc agtagtgaac tggtttacaa cggcaaaaag gataaaagag tagattatta
     1681 tttacacttg ttttttgcat tgatacaacc ttatttaacc aaagaagggc aaaagcaata
     1741 tttaaggtta aataattaac ggaaaactat ttacttaatt tatttattac tgatttgaag
     1801 tatgcttttt cagtatcact ttttgacaat tttatagcaa actcccaatg gtaatttgct
     1861 tgcatttggt tcacttcatt gtataatttt cccagtaaaa cgtgatacat tttattgtta
     1921 ataagtttta atttttcagc gtccaaaatt ccttcatcta ctccgtttac tttggacaac
     1981 gcaaatgttc tgttaagcgc cgtgatagga gtatattgaa tttgtaataa cttattatat
     2041 aattgcaata tattttccca tttctcaaca gtatcctgtt tatgtgtatg ccaataggca
     2101 atagcggctt caatatgata agtggatatg tggctacctt ttgccgaatg attcaaataa
     2161 tactcccctt tttgtattaa aggtttattc cacagttgtt catcctgctc ttcatacaaa
     2221 acaatttcac cattgttatt gctgcgtgct tcgaagcgag atgcatgaaa acacattagc
     2281 gcaagcaaag catttgattc agcggtattt gtgttttgat gttcaagcaa taatacattt
     2341 aaccgcattg cctctaagca caaatctttg cggagtgttt tattttgtga ggctgaataa
     2401 tacccttcat taaacaataa gtatatggtt gtaagtactg ctgataaacg taaaggaatt
     2461 tcgttttgaa caggaaaatc tatggaaata ttttcatctc tcaacttttg ttttgctctt
     2521 tgcaatcgct tataaataac atctcgattc gtaagaaatg cttctgcaat ttcatctgct
     2581 ccgaatccgc acaaaatatt caaagctaaa ccaacttggg cttcaacagg aataatagga
     2641 ttacaaatcg caaacatcat ctttaactga ctatccatta tgttggaagg agaaaggtct
     2701 ggctctggaa cataagccgt tggagaagca acaagtattt ccggatatac cttttgttta
     2761 aatacctgat tatgtttaat gaaatcgatg gctttatttt ttgccacagt gtacaaccat
     2821 gcagtaggat tatttggcaa accttttaat ccccatgtat tacttgctaa taaaaatgtt
     2881 tctgaagcaa tgtcttctgc ctgttcgaga aaaggcatac cgaaatattt acataaaaca
     2941 gcaacaattt tactgtattc ggttctgaat aaatcgggta ataagctagg ttgctccata
     3001 aaaaaataac ctcccttttt acagggaggt tataaagata tacaattgat taatccattt
     3061 tgataatcat tctgatttct acggttccgc caacatctaa aatggggcaa ccatgggaaa
     3121 gttgagttgc ttcgtcaatg gtttcagctt ttacgataat ataaccgcca atagcttctt
     3181 ttatctccac aaaagggccg tcggtaatta cattattggt agccagcact tttccgcttg
     3241 gtgctaaacg gttgccgggg ctcaccaatt tattttgtgc tgcaatacca ccaatccaat
     3301 ccatccaggt tttcatcatt gtctgcattt gatcgggcga catttgtgtt tgcgataacg
     3361 caacatcatt tctgaatact aataaaaaat cgttcataat cgttataggt ttttaaaatt
     3421 aattgttgga atgtaatgcc actgaattgc cttcagtgtc gatgaaaaat gccatgtgcc
     3481 caacattatc agcaataaat gttttaggca tggtaatttg tccgcctgca gcttccactt
     3541 ttgataatac attttctaaa tcgggattac catttaaata aattttggca ccatcggcac
     3601 ttggtttatg gaattcgctt tgaacaagtg cacctgacac tttaccattc atatcttcat
     3661 atggaaagta agccatttgc atgcccatca tttcttcctg gtgcatttgg ataccaaaaa
     3721 tactttcata aaactttttt gcgcgggcaa tatctgtaac cgatatttca aaccagttga
     3781 gcgcgtttac gtttgcattc ataatgattg tttttgtttg ttaattgaat atttactcct
     3841 ataacgattg aacttttcgt attggacaaa acaaccaaaa aataattata agacccgtta
     3901 attcgtgtta aatttatttt gcccgcattt tatttccttt ggcatcgtgg gttacttctg
     3961 ccagtccgag tgcctccata agcggtggaa tatacatccc aaaacgacca cggaatcctt
     4021 tttttaagcc ataccaacca ccaacaggat ttttttcgga acgcccccag gcttctacag
     4081 tgccttcttt agccggcttt tgttcatcgg cactgcctaa ttccatccag tcgccgtgtt
     4141 tttttaacat ttgatgcagg tcgttcaaac aacgtatatc ataatgcaaa atcgttttac
     4201 ctactgtgca tactaaaatt tctttaccat ctttttcatc cacatgcatg gtatattccg
     4261 atgaaagtgg tggtgttttg agtgtaaggg gtttgtcttt ggtgccgatt ttatttttca
     4321 tgttaattaa attttatctg ttaattaatt atttagtttg atgaatgatt tctgcttcct
     4381 ttttcaaatc tgcagcaaat tgttcgaaag atgcatcaaa agaaggaata tattttgcat
     4441 acatcgggaa catcagtcca ccgatttttt cggtcatggt aaataatacg ccaccggttt
     4501 cattgggtgt aatggtgtaa gtgcggtcgc ctttgccatc tccccaaatt aaagtgcttt
     4561 ctttttcaag tgattttact ttgagtttga acgttcgagt tgtgtctagg gtagatttca
     4621 attgaatttt ttcgcccaaa gcaatattac catcaataga tacaatagtg ctgttccaac
     4681 gaggaaaatc ggatgcattt gtgagcagac tccaaacaat tgcctgattt gcctgaatat
     4741 taatgctaac ggttgtttcc ctgctaaaag tggttttagt ggttgtggct gttccgtttt
     4801 gagccattga aatgtttgct actgtcataa aggttaaaat tgtgatgaac tgtttcattt
     4861 tgtttgaatt taccttttga cgactggggc agaaaaaacg ataggctaaa cttcattttt
     4921 tttcaaaaaa ttttccataa cccttgaatt atgggcataa tggtgcagtt gaagctcggc
     4981 tgaagggctc tcgaaggggt cgattccctg cacaatttgc atacggaggt cgaacaaatg
     5041 ttctttatcg ggattatttg cctgttttgc catctcaatt ttgttcaact cttcctgatg
     5101 gttctgtttg gggttaaaaa taccattgag ttcagtgcat tggtgacaaa taccctcctt
     5161 attaataagt gcacatcggc cttcaaaaat ttgaaccagt ttagcacggc cagtgtgcag
     5221 gtaatatttc accatggctt ccgtagtatt tacaatttca gtgatttcat ttaccttgaa
     5281 ttcatacact tctttgagca ggatacatat ttgttgctcg agtggtaatg attttgcaat
     5341 acaggtaaaa caaaacgtga tatgttcttt tatttcaaaa gcgccgtgag gtgaagtcat
     5401 acgaatttgc attgcttcag caaaaaagtt tttatcggtt aatgcagcag ctttcccccg
     5461 gtcagttaca tcctctgtcc atcttcgttt tgcccgcaaa ttatctttcg ccaaattagc
     5521 tgcaatagta aaaatccatg tttttaaaga ggactctgca cgaaaggatt caattttttc
     5581 tgatgccttg atataggtat cctgcataat gtcttctgca tcttcaacac tggccgtaag
     5641 gcgcagtaaa aaagatttga gctgtccttt atgttgcaaa aattcgttgg taagttgggt
     5701 tgtttgcata aataattaag gttgttaaaa gacattataa tatcaaatat aaatatttat
     5761 aatctattca aacattaaat aaatattatt ttaataccac aaatttgtca atggcgatgg
     5821 tttttgcttc atctttaatt gtaactgcat aggcaccatt tgttaaatgg tttacatcaa
     5881 tcattccatt tttaggtgtt gaaataacgg aaactatctc accaaacata ttatatatag
     5941 tgatagttgt tgcattatcc ggtaatccat ttatcgaaaa ataatccttc accgggtttg
     6001 ggtaaatagt taatgttgtt aataaattat catctgaaat tgctgtaata cattccacgt
     6061 caacatcact aaatccatgt aatgcagcct ggcataataa ttcctgcatg gcgggtttat
     6121 cagtccccat accttcatat gttcgcaagc caattgtgcg caggtattct aaaaatactt
     6181 catcggttaa tccgtaataa tcgccccaat aatctaccat taccgggtca aggtcgtgta
     6241 accaggtaaa atcgaatcct ttaattgcag ttgggtgcat atcccaaatc tcaaatgttt
     6301 tagtaataaa atcacgttgc aaaaaatcag agctattaca taatgtactt gaaggatatc
     6361 catattgata aaaaaacaaa ggtttgtcag gatataaatc aattaaagct tctatatcat
     6421 ctgcaatagc cgtgacaggt tttacggtga aatcaggatt taaaggataa tacgaaacac
     6481 caatataatc tgaatttgta tttacaattt gtgcattggt attatataca attaaaccgc
     6541 caaatgtaat ttctgttgca attttagtat cgggccataa tgtttttgca tgaatgacaa
     6601 tagcattata aaattcagcg taattattcc aaagtgtggt attacttcct aaatacacat
     6661 catattcgga gcctattact aaagagttta aagttacatc tggaatatgc gcatgcacac
     6721 tatccaacat agctttaaat cgactaataa atgttggatt gttatatgga gttgccacta
     6781 aatcggccgg tacttctaaa ttattagtat gtattggcgt aatagttaaa tcgagtggca
     6841 tattataagc aggataatac aaattggcaa tatcaaataa actcagatta taagtgagtg
     6901 gtgcaacttc aatcattgtc caattttgat ataacccaac agagccaata cctgcatcaa
     6961 cacctttttg aaaacaagaa tcgtaggtaa aagcaggatt ttggggggaa gtgctgatat
     7021 ccaagctgat gatattatta tccggattaa tttgagctga aagtttgtaa tggatgaata
     7081 tcagcagggt aattaatgaa atatatttaa ttattgattg cataaaaaat attcgttatc
     7141 gatttaataa atggtaattt aatgtttaaa tagccgctgt cgccattatt tctatcaaat
     7201 atttcggatg tgccagtttt gcttctacag tggctcttgc aggggctaat ccgggtgaaa
     7261 tccatttatc ccaaataaca ttcatataat cgaattgggt gatgtccggt aaataaatag
     7321 ttacttgcaa cagcttattt ttattactgc ccaaatcgga aagtgtgttt tccatctttt
     7381 gcaaaacact ttccatctga cttttaaaat caatggcatc ggcgtcggca ataattccgg
     7441 acaggtagat ggtattatta tatacagttg ctctggccaa tcggttgccg ggcatgtaat
     7501 attgaatggt gttggtagac ataacgggta gtaaaaatgg taaattttaa taaatattgt
     7561 gctcctcagc tttgggaaca tttgcgttta gcacttttgg attgcatagg ttaacaaaat
     7621 cttggaatat tttgctttac caacaccatg ttaatatccc ccaaatttat ttatcttacc
     7681 tttatagccc gttatgaaga atgatcaagc tcgctatatc ttcgttacgg gtggggtaac
     7741 gtcgtcgctg ggaaaaggga ttttttccgc atcattagca aaattattac aggctcgcgg
     7801 tttcagggta accattcaaa aatttgaccc ttacatcaat gtagacccgg gaacattgaa
     7861 tccttatgaa catggtgaat gttatgtaac tgaagacggt gcagaaaccg atcttgacct
     7921 tggtcattat gagcgttttt taggaattga gacatcacag gcaaacaatg taaccaccgg
     7981 tgccgtgtat cagaatgtta tcaaccgtga acgtgcagga gaatttttag gtaaaactgt
     8041 tcaggtaatt ccacacatta ccgatgaaat tcaacatcgc atgttgcttt taggcgaatc
     8101 gggagagttt gatattgtta ttaccgaaat tggtggaacc gttggtgata tagaatcgct
     8161 gccatatatt gaggctgttc gtcagctaaa atggaaactc ggagaaaaac gttgtatcat
     8221 catccacctt actttaatac cttatttgaa tgctgcgcac gaattaaaaa caaaaccaac
     8281 tcagcactcg gttaaactcc ttttggagag cggtttacag gctgatatat tagtttgtcg
     8341 tacagaacat ccattaccaa aagaattgcg cagaaaaatt gctcttttct gtaatgtgaa
     8401 tgtaaactct gtatttcagg cattagatgt aaaaaccatt tatgatgtgc cattacatct
     8461 tttagaagaa aatgcagatg aaattgtatt aacaaaactc aaattaccta aaaggaacga
     8521 accggattta aaaaattgga aagcattttt atcccgttta aaaaatccta ctaactcagt
     8581 aaaaattggt ttggttggaa aatatgtgga gctgcaagat gcttataaat cgattttaga
     8641 atcgtttatt cacgcgggtg ctgctaacga gtgcaaggta aaagtgattt ccatccaaag
     8701 tgaacatatt accgattcca atgtagctga aaaattagct gacttagacg gagtgcttgt
     8761 tgcgcccggg tttggcgatc gcggcattga aggaaaaatt acggcaatta aatatgttcg
     8821 cgaaaataaa attccatttt tcggtatttg tttaggcatg cagtgcgctg tgattgaatt
     8881 tggccgaaat gtgcttaaac tcaaagacgc gcatagcact gaaatgaatc ctaaaaccaa
     8941 acatccggta attcacatga tggaagagca actggaagta accgaaaaag gtggaaccat
     9001 gcgtttagga agttatgatt gccgtttgtt taaaaagacc aaggcttacg aaatttacaa
     9061 atcacaacat attactgaac gccatcgcca ccgttatgaa tttaacaatg tatatttgaa
     9121 agattatgaa gcaaaaggta tgatggcaac agggctcaac cctcagcaaa acctggtaga
     9181 gattgttgaa ttaaaggacc acccttggtt tgttgccgtt cagtttcacc ctgaattaaa
     9241 aagtaaggtt gaaaacccac acccgctttt tgtagggttt gtgaaggctg caatcgactt
     9301 taaagaaaaa aagcaggatt cagtaactgc tgaacaataa acccgagatt gcctgaaata
     9361 aaggctttcc gactacaata tgggctcgtt accaatcgtc attgatggtg cgagcccata
     9421 acattttcaa ttttctttgc caatgggagc gcataaccta actttgccgc tcaattatca
     9481 ataatcaatg gatcgtaata atgtaatagg cttcgtgtta ttaggggtgt taatcatcgc
     9541 cttcttcttt atgcaaagcc gttttgccaa tcaacagcga gttgccgaag cccaaaaaca
     9601 agcaacggaa gactctatta aacgcgtaga agattcctta aacctgctca aaaatccaat
     9661 tttagaggaa aaaaaggaaa ccactttatc gaatgcagct gttgccatac ctgatagctt
     9721 aataagtact ttaagtgctg atgcaattgc caaaatggaa gattccattc aacaattaca
     9781 gaaaaaaaat gtgtttggca gattttcagc tgtagctcag ggaactgaaa aattagactc
     9841 cattgaaaat gatttaatta aagttgtatt taccaacaag ggtggtcata ttgttaaagc
     9901 tgaattaaaa aaattcaaaa attaccacgg tggtccggta gatttaatta gcggaaatga
     9961 taaccgtttt aattataatt tcaaatacaa caacgataag atgattaact cggaggagtt
    10021 gtattttaca gcctccaaat catcagatgg caagtcaatt aaatttattg ctgatttagg
    10081 agaaggaagt gtatttgaac aaacttatgc atttacagca accgattatt taattgatta
    10141 taacgttaat ttcaaaggct tcgaaaatat tatttcccgc caggacccaa ttattatttt
    10201 gaattggaaa aatgttatgc aggagcagga aaaagccctg aaatatgaac gccaatattc
    10261 aaaattatac tttagtgatg ttgacggtga tgtagattat aaaaacacca atggtgaatt
    10321 aaaatttgaa actccggtaa aatgggtaag ttgtcagcaa cagtttttta ataccacttt
    10381 aattgctaaa gaaagttttg acagaaaggg taaaattgaa gtgtttgcag atgacacgtc
    10441 tgcattcgta aaaagctgta atactgagtt ataccttact tatgatggca aaaaagattt
    10501 tactgttcct atgcaatggt atattgcacc taacgattac cagtcgctaa acagtatgga
    10561 tttgggaatg gaaaccatca tcccgacagg tagtggattt ataggctgga ttaacacgca
    10621 tgccattatc cctttattta actggttaag tggaatgatt acgaattacg gtttgattat
    10681 tttattaatg acgttaatca tcaaacttgt actaacccca cttacttata agtcgtattt
    10741 atcaatggca aaaatgcgcg tattaaatcc tgaattaact gaaattcggg aaaaatataa
    10801 agatgatcag gcgcgcttag gacaggaaca attaaagtta tatagaaagg caggtgttaa
    10861 tccaatgggt ggatgtattc ctactttatt atccatgcca atattaattg ccatgttccg
    10921 tttatttcca ggaacaatag atttacgtca gcaatcattt ttatgggcag atgatctttc
    10981 tacatatgat gacatcatta gttggggcac aagtattcct cttattggca accacatcag
    11041 tatattctgt attctgatga ccttgagttc ggtattatat attcgtatga atatgcaaaa
    11101 tacggctcaa ctgcaaggac caatgaaaac tgtgcagtat ctcacaccgg tatttttctt
    11161 ctttttccta aatagctcac ctgctgcttt aacttattat tattttgtaa gtaatatcgt
    11221 aacatttggt cagcaatggg caattcgtcg tttctttatt aacgatgacg acattcacaa
    11281 aaaaatacag gaaaacaaag ctaaacctga aaaacaaaaa tcaggatttg ccaaacgttt
    11341 agaagatgca atgaagcagc agcaaaaaat gcgtgaagtg cagcaaaacc agcctaaaaa
    11401 gaaaaaataa tttttagtgg taatgatttg attttgagtt cgtattgact ttttttagac
    11461 ataactgaac ttagttggta gattatacct tatatttacc taaaaagtag ttatgccctt
    11521 aagtctttcc agtttaaaac aaaggaacaa tgttgttctg cttgttacca tttttcttgg
    11581 cgctatcatt gcatttggcc taaaccgaat tttaggtggg ttactaggtg caattatcat
    11641 atatgtatta tttcgcccat taaatatcta cctccaggag aaaaaaaact ggaataagag
    11701 tttaagcacc agtattattt tagtactttc cttcttttgt ttaatagttc cgatattttt
    11761 attggttact ttaataactg ataaagcatt gtattacgtt aatcaccctg aacttactga
    11821 tcaaatgttt ggcaacctta acaagttcgc cgaggaaaaa tttaatgaac ccgatttagt
    11881 tggtaattta ctcgaaacac taaaagcggg agcaggcact tttgtttcta ctgtagtaaa
    11941 tggagctgcc aatacattta tacaaattat cgtgatgtat ttcaccttgt tttttacgat
    12001 taaaaatttc agggagtttg aaaagggttt aacacagtat ttaccttttc gcagagcaaa
    12061 ttcaataagg atagggaacg aattacgcaa tatgacctat tccaatattt taggtcaggg
    12121 ctgtatcgcc attattcaag gcgctttatt aggtttagga ttttggatat ttggtatacc
    12181 ggacccattt ttctggggag taattggcgc ttttttaagt atgattccat tattcgggtc
    12241 accgcttatt tttttaccgg caggtattat tgaaatgtca aatggtaata tggtttcggg
    12301 aataggcatc attgtgtatg gttatctgat agtaaccatg gtagacaatt tcatccgcat
    12361 ggcgattggt aaaaggatag ccaataccca cccattaatt accattattg gagtggtaat
    12421 aggtatacca atatttggta ttttaggaat attatatggc ccgctcatta taagtttgtt
    12481 tattattttg gcaaaaattt atcgcgaaaa cagaaacgaa attatacagt tatccggtga
    12541 ggatgacaat acttaaattc aatattatgg aaaaatcaaa caaccctaat ttaagtgaaa
    12601 atcttaatga ggctattgat gatgtaaaag agtatgtcaa tttgcgcatg caactcattc
    12661 aattaaatgt caccgaaaaa gtaagtattg gtcttgcggg attaattgcg ggaggcattg
    12721 cacttattct atttgtttta tgttttattt ttggctcgtt tgccctagcc tatttgcttg
    12781 gaaatgtgtt acacaacacc gcagcaggtt ttggcgtaat ggcgggattt tatttattac
    12841 tcggtattat tatgctcaaa gttggcaaag gcaaacttcg cataacactc attaattcat
    12901 ttatcaaaca atttagtaac gactaatgtg tatttatgga accgataacg ataaactcag
    12961 tagaagattt acagcgtgca aaagcacaac tacgtgttca aataaacaat aaggaacaag
    13021 atttaaaagc tcattataaa actatttcca accaggtagc acctgcatta cgcattgcta
    13081 atgtagtttc cggtaatcgt ttatttaaat ctgctttagg tggtaccgat gaaaatgaca
    13141 atggttggtt aagttcagca atgaaaattc tgacagcggt atcagcaggc ggatttattt
    13201 tacatcaaaa caaaaaaaat cttggcaaag cattacttgc ttatgcatta gatcaaggtg
    13261 taaaatatat taaggagcac gatttgtctg aacacatcga caaattaaaa aaatggttga
    13321 atttggaaac ggaacaaaca gtaaataata cacaggataa cgattctgaa gccgaataaa
    13381 ttatttatct gttaaatagt aatcgcgcac cgtttgttcc ttcaacaaac ttatcattgt
    13441 cgtatactaa atttccgttt acgaatgttt tttcaatttt ccctttaagt gtataacctt
    13501 caagcggact ccatccacat ttatagtgca acgaattggc tgtgattaat tgaggtttat
    13561 cagcatccaa taaaaataca tcaccgaagt atccttcgcg aataaatcca cgatctttta
    13621 tttggaaaca tgtagccaca ttatggctca ttttttcggc aatttgcggc aacgtaatct
    13681 gacctttatg aaaaaaatcg agcatgagca ttaaactatg ctgaaccatt ggtgcgccgc
    13741 cggggcattt actataaaga tttgcttttt cttccaaggt atgcggagca tggtctgtag
    13801 ccacaacatc taaggtgcca ttgagcagcg ccttgagtaa ttccggttta tgacgtttat
    13861 ctttaactgc aggattccat tttatttttg caccaagttg tgcataatca tctgcatcaa
    13921 aaaataaatg gtgcacacaa acttcagcag taattttttt ctcggataat gcaactgcat
    13981 tagaaaacaa tgccaattct tctgctgtgc ttatatgtaa aatatgtaat ctggtattac
    14041 actttttagc taattcaatt gcgaatgaag aggataaata acaggcttcc tcgtttcgga
    14101 taatcgggtg catgtgaaca ggaatatttt cgccatactg ttctttagct ttatcgaggt
    14161 tgcgcaatat agttgattca tcttcacaat gtgtggcaat aagaaccgga gcatttttaa
    14221 aaatgtgttc cagtgttgcg cggttatcta ccagcatatt accggtagaa gaccccataa
    14281 atattttaat accacaaaca ttggttggat tagtctgcaa aacttcttca gcattatcgt
    14341 tgctcacacc catataaaat gaataattcg ccaacgattt tgttgcagcc aattgataac
    14401 gttgttccaa caattcttgt gtaagtgaag gcggatttac attcggcata tccataaatg
    14461 aagtaacacc acctgcaaca caagctctgc tttcggtaaa caaatcagcc ttatgtgtta
    14521 aacccggttc gcgaaaatgt acctggtcgt cgataattcc cggcattaag tatttgcctt
    14581 cgccatcaat ttcagtatag ttaccatttg tttgaatgat acccccaatt tgcagaatac
    14641 ggttttcgtt aatcaataca tcgccttcgg caattttacc ttcattaact attcgtgtat
    14701 ttttaattat ggtcttcatc caaatactaa ctaaaattta cattaaaaat tattccaccc
    14761 aatctgcaat aaataaattc gtatcgcgat taccaccggt atttctgtta cttgcaaaaa
    14821 tgagtttttt gccatcataa ctaaacattg gaaacgagtc gaatacagta tcaaaagtaa
    14881 tttgttctaa tcctgtacca tctaaattaa tcataaataa attgaacggc acacgtttag
    14941 tagtatgatt gcttgaaaat ataattttat ttccggaagg atgaaaaaac ggagcccagt
    15001 ttgcgccgcc caattgcgta atttgtctta aatcagaacc gtcagcttta caagtaaata
    15061 attccattgc tgtaggttta acaaaaccat cttttaataa cgacttatag gttgcaatat
    15121 cttccggtgt ttttggtctg ctggctctga aaacaattct atctccttca ggtgaaaaaa
    15181 atgcgccgcc atcataacct aaatcaaaag tgatttgttt taaatcgccg ccatcaagat
    15241 tcatggtcca caattctaaa tcgccgcttc tgtcgctggt aaataatatt ctatcccctt
    15301 ttggagaaac aacagcttct gcatcataaa aattattatc cgttaattgc ttaacgatat
    15361 tgccttttaa gtcagccaca taaatttcat aacttgggta cacaccccac aaatatcctt
    15421 tacttttatc agggtcagct atacatgtat ccacttgagc atgagtagaa gcaaaaatca
    15481 ctttatcgtt tcctggtaaa aaataagaac aggtagttct tccagtaccg gtgctcaaca
    15541 atttatattc aaatcgcgaa ttgtcggtag gaattttacc ataataaatc tggtcgcaag
    15601 gaacttcctt attatcacga cgctgaaata caatcatttc actgttaaaa ctaaaatacg
    15661 cttccgcatt atctccgcca aaagtgagct ggcgcatatt tttgagatgt ttttcctgag
    15721 ggtaagttat ggtatctacc acaactgtgg tttcctcttg tggtttttca acttttgatg
    15781 attgtttgca acctgcaatt ccgcctgttg taattgcagc aattaacact aagtgagtaa
    15841 tctttatcat attttggtaa atctgggggc aaaattaagc acatcgcatc atttagccga
    15901 acattagtcc gttaaatatg tctaaaatcg taaatttgcg gtatgatttc caaaaaaggg
    15961 cttgtaatcg gaattattgg cgcagctgta attacagccg gaattttatt ggcgaaatca
    16021 aacgttttta aaggtggaaa taaaaatgaa gtggcggtcg acagtctcga aatggctgaa
    16081 caactcacac ctcaaattgc ggatttatcg ctccaaatta acggcaaccc tcaaaatgcc
    16141 ggattatatt acgccagagc taacgaatat ttcaattttg gcaatttgaa atttgcgttg
    16201 gaagattata aaaaagcctt tttgttagac tcaaccaatg ccactcttgc cctgggttta
    16261 agcgattgtt tattcgaatt aaacaatgct gatggcgcaa ttgggatttt gaaggattat
    16321 ttaaaaaccg atcctgaaaa tatcgacatt atgctcaatt tggctatcga ttattattta
    16381 ttacctaaac ctcaatatca aaaagccatt gaagttttaa acgatgtatt aaaagttgat
    16441 gtgcaaaata gtgaagccta tttttataaa ggcatgattt ataaagaaac aaccgataca
    16501 accaaagcca tttcaaattt tcagactgcg gttgaagcag accctgatta ttacgatgca
    16561 tatatgcaat taggtctttt atatgctgag cagaataatg atattgccat caaatatttc
    16621 gataatgcca ttgcattaaa cgacggcagc aacgaagcgc attatgcaaa ggccaagtat
    16681 tatcaggatc gtggccgaat ttcagaagca attggttttt ataaaaagat gatagttgcc
    16741 gacccgcaag atgccaatgc catctataac ttagccacta tttattttgg cattgattcg
    16801 atacagcagg cttatcggtt ttatgaactg gcaattaaac aggctccggc taaggcaatg
    16861 ggctattatg gaaaaggatt atgtgctgaa gaattaaaaa ataaggaaga agctatctca
    16921 ttatataatc aggcattaaa tttagacccc gatttgaaag ttgcagaaga gcgattatta
    16981 atattaaatg aaaaataaaa gatgattaag attacatttc ctgacgggaa tattcgtgag
    17041 tatcaggagg gcgtaacttc aatggaagtt gccaagtcaa ttagtgaagg attggccaga
    17101 aaaattttaa gcgccgaagt aaacggcgaa gtgtgggatc tgagcagacc aataacaagt
    17161 gatgcaacgg ttaaattatt gacgtgggat gatccgaagg gtaaaaatac attttggcat
    17221 tcaagcgctc accttatggc tgaggcattg gaagctctat atccgggcgt taaactgggt
    17281 attggcccgg ctattgacca tgggttttat tatgatatcg atttaggtgg ccgtgtttta
    17341 acagtggaag agttgcctaa aattgaaagc aaaatgaaag agttggctaa aaccaattct
    17401 gcctatatcc gcaaagaagt ttcaaaagct gatgccattg aatattttac tcaaaaaggg
    17461 gatgaatata aattggagtt aatcagcgac ctggaagatg gcagtatcac attctattca
    17521 caaggcaatt tcgtggattt atgccgtggc ccgcatattc ctgaaaccgg cgtgattaaa
    17581 gccgttaagc tgttgaatat tgccggtgct tattggagag gtgatgaaaa aaataaaatg
    17641 cttactcgtt tatacggcat taccttccca aaacaaagcg agttagatga gtatatggtg
    17701 atgttggaag aagcgaaaaa acgtgatcac cgaaaattag gtaaggaatt agaaatttat
    17761 actaccgatg atgaagttgg tcagggtttg attttatgga tgccaaacgg cgcagctatt
    17821 atcgaacaat tggaaaaact ggctaaacgc aaagaggaac aagcaggcta tgtgcgcgta
    17881 aaaacaccac atattgcgaa ggaagacctc tatttaacca gcggtcactt accatattat
    17941 gctgatagta tgtatccgcc tatggaaatg gaaggggtga aatattacct caaagccatg
    18001 aactgccctc accatcataa aatattcagt gctgcacctc gcagttaccg cgatttacca
    18061 ttgcgttttg ctgaatacgg tatgtgttac cgttatgaag aaagtggcgc gttatttggg
    18121 ttaatgcgtg taagaggatt aaatatgaac gatgcccata tttattgtac cgaagagcag
    18181 ttcgaaaatg agtttaaagc ggtaaatgat atgtatctga attatttcag aatttttggc
    18241 attgataaat atgtaatgcg gttttcgacc cacgagccta aaaaactcgg gcagaaatat
    18301 ctggacaatc cggaattatg gaaaaaaacc gaggatatgg tgcgcagagt gatggacaat
    18361 gcgggtattc cttacattga ggtacctgat gaagcggctt tttacggacc aaaaattgat
    18421 attcaaattt attccgcaat tggtcgtgag tttacattag ccaccaatca ggtggatttt
    18481 ggacaaccat tacgttttgg cttaacctat accgatcgcg ataatcaacc gaaaatgccg
    18541 ctggtaattc accgtgcgcc tttgggaaca catgagcgct tcatcggctt tttactggaa
    18601 cactatgccg gtaatttccc ggtttggctg gctccggttc aggttgctat attgccaatt
    18661 agcgataaat acaacgaata ttgccaggaa gtaataaatg ttttaaaaaa taacgaaatt
    18721 cgcacttact tcgatactcg cagtgaaaaa atcggccgca aaatcagaga tgccgaggtg
    18781 aaaaaaattc cattaatgct tattgttggt gagcaggaga tgaattcaaa aaccttatca
    18841 ttgcgtaaac atggcgaagg c
//
LOCUS       DAORKW010000017        18645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_27_length_18645_cov_4.675309, whole genome shotgun sequence.
ACCESSION   DAORKW010000017 DAORKW010000000
VERSION     DAORKW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18645
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27_length_18645_cov_4.675309"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1522)
                     /gene="glmM"
                     /locus_tag="PLB46_01585"
     CDS             complement(140..1522)
                     /gene="glmM"
                     /locus_tag="PLB46_01585"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRF75231.1"
                     /translation="MTLIKSISGIRGTIGGKAGQNLTPEDIIKFTAAFGTWIKNTTGN
                     PTIVIGRDARISGEMVNHLVTGTLQSIGIKVIDLGLSTTPTVEIAVPREQAGGGIIIT
                     ASHNPKNYNALKLLNHLGEFISGADGEAVLAIANSEEIVYSDVDKLGEYQHKFYYIQK
                     HIDKILELPLVNKKAIKERNFKVVIDCVNSTGGISVPELLVALGVTDVVELFTNPNGR
                     FPHNPEPLPEHLGTIAHEVKHQKADVGLVVDPDVDRLAIVCENGDMFGEEYTLVSIAD
                     YVLKHTPGNVVSNLSSTQALRDVAAKHKVEHHQSAVGEVHVVNMMKEVNAIIGGEGNG
                     GIILPELHYGRDALVGIALFLSHLATSGKSCSRLRADYPNYFMAKNKIELPEGTDVQY
                     ILRKMEEKYKKEKLNKTDGLKIEMGGDWVHLRASNTEPIIRIYSESSTKTTAENINKK
                     IMDDIKNVMK"
     gene            1610..2344
                     /locus_tag="PLB46_01590"
     CDS             1610..2344
                     /locus_tag="PLB46_01590"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF75232.1"
                     /translation="MAVTKNWFETWFSQGYYDILYQHRDEDEAAGFLDALMEKLQPPT
                     NATILDAACGKGRHAIYLAEKGWDVTGIDLSYNSIKEAKLAENEHLSFFQHDMRNVLR
                     VNYFDFIFNFYTSFGYFANIKDDIKCIRSFANGLKPGGKLVIDFLNVFTTLQNLVEDE
                     HKVFGDIQFDITKSYANGMISKKIEVKHKNQHHTYHEHVRALQLQHFDHYCSLAGLKI
                     VATFGDYDLQPYNKLQSERLIIIAEK"
     gene            2341..2928
                     /locus_tag="PLB46_01595"
     CDS             2341..2928
                     /locus_tag="PLB46_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRF75233.1"
                     /translation="MIFPEFIQQFDEWAFLLINNKWSNHVFDIIMPPVRDKNFWIPLY
                     IIIAIVLVYQYRWKGLTLILLLGLNFGVSDQLSSAVIKPAVGRVRPCNDESFKEQVTL
                     RIERCGAGKSFTSSHATNTFAFAIMLTLLFRKKYRWIAPVALFWASLVSYAQIYVGVH
                     YPIDILGGALLGTGISLIIYKIASRYVLPKLGPEL"
     gene            2954..3712
                     /locus_tag="PLB46_01600"
     CDS             2954..3712
                     /locus_tag="PLB46_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRF75234.1"
                     /translation="MSILQIVFIISASVAGILAAWFLRKQTHSELLSILLSFSGAYLL
                     GNVVLHLLPEIFADAHAGHQETGAHAHDYTAAIYILLGFFIQLLIVQFTRGIEHGHLH
                     LHEHQSKGYIAGVFFGLSFHAFMEGVPLVGNNMEAAQMQPLFNAILVHKIPEAFALAT
                     VLFFSVSKQLNAYLLLAFFALMTPLGSYAGNYLTTQHVMDFKWLIAIVCGSLIHISTT
                     IIFESSGKAHKISMYKFIAILAGAGIALIAAMME"
     gene            complement(3716..8719)
                     /locus_tag="PLB46_01605"
     CDS             complement(3716..8719)
                     /locus_tag="PLB46_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRF75235.1"
                     /translation="MKKHLLLFFFILSILQVNAQTYHNEWIEYDQQYLKFPIAKNGIY
                     RISYDVLNSAMNDIGVPLATVDPRNFQLINKGVEQYIHISGEGDGVFNAADYIEFYGE
                     KNDGTFDSLMFEFPEYQLHQYESLITDTAMYFLTWNDLITNHRLEVVINDLTDTPAPL
                     LYHDYHTRIVYGAGYGSSNFNIGDPFNDILSSDMTEGEGYATGKYNLSNFNVTVNTPD
                     FYAGPSFIPRLKTALIANGDGEHHFLIKWNGEIIKDTTFFNYRVGRYEFNLADILAAN
                     TITFSSGPVSTDYQRYSFIDVNYPRSFSCNNESVLKTWLTQVVGATTYLECTNFNEKF
                     TSPILYDLSNHKRIEAIVEADISKFHLDYNATNAELYFSSQDATDIFSVPKLNPVTFV
                     NYGATANQGNYMIISNKVLFEDVDGTNWVNAYRDYRASIAGGSYDAIVIDIDVLYDMF
                     SYGLKKHPLAIRNFVTYAVDNFVTAPEFLFLIGKSYSYDVTRANGTPEYAASLVPTFG
                     NPGSDNMLVTRTGDFVPLVAVGRISAKTPNDVRIYYDKIIEFEAQQADHTQTIANKAW
                     MKNVLHFAGGASEFEQTLFNSFLNQYKNIIEDTLYGGNVIPFNKLNADPIFYSESAYV
                     DSLINTGVSLITFFGHSSTGSFDYNIGSPNEFNNEGKYFAVYGNGCNTAAIHGDAYTL
                     GEQYIFAENKAAIAFLAASNFSIAANLHTYATLFYRELASNSYNLPMGIAMRAVADTL
                     WPTDNPFDRMTIQHNTLQGDPAIRLNTHDKPDYAIEAPYVHFEPDVISAGTDSIYMHL
                     IVTNLGSAIDSSYFVEVKRTKFGGETSTFFERFPATRFRDTLIIPFATDGLAGAGLNN
                     FAIHLDKENEIGEIDEFNNILNTTLFVISDDAMPIYPVEFTIMNHVPEYFAASSTDAF
                     APVKQFIFEADTTTLFNSPLRKTTMVTSAGGVIKWENPPLTYIPNVVYYWRITPDTID
                     GSEAMWRSSSFLYLPGEITGWNQSHYYQYLEDEFTNVNLEPSREFTFVPDVKTYEVAT
                     GIYPTTHWTEVTSYADGELLAIGSCASTGFVVLVADANSAELWQTSEVGATNLGPYGD
                     VYCSADAYERVIQFYTNSPESREKLYNFMMNELPDSTHFICYSNNYPDFYAWLDDTLI
                     YGHSLFDAFNAYGATDINALSVFDYDRSYIFYAQKGNTDSKVEIIGDPEGHKIEATFV
                     INGNWNKGNIETPLIGPAEEWEKAQWSFYANDLPGVDTNSISVIGVKINGVETVLKTG
                     LQSGDTSITFADADLYPYIKLRLETKDDTLRTPAQFNYWRVLYDPVPEAAVNPNLHFA
                     FTNDTLQQGAPGSLELAITNVSDYNMDSLLINFGIFDANNFYHEVPYSRQDSLLSDES
                     MIATIQFNTNQIPIGRSTLVAEVNPKNDQEEQFHYNNFAYLPVQVIGDAKDPLLDVTF
                     DGVHILDGDIVSAKPQIIINLKDENPYLALADTSLLKITFQYPNYSFHDYYYDGVTTK
                     FYPADPSNLTENNSARVEINPIFSMDGTYELRVQGMDNTGNTAGDIDYTISFEVINKA
                     MVSNVMNYPNPFTSQTRFVFTLTGSEVPEYFKIQIMTVTGKVVREIMRSELGELHIGN
                     NITEFAWDGTDQFGDPLANGLYVYRVVTRLHGESIEKYDTGTDQYFNSGFGKMYLAR"
     gene            complement(8750..9847)
                     /locus_tag="PLB46_01610"
     CDS             complement(8750..9847)
                     /locus_tag="PLB46_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRF75236.1"
                     /translation="MRKYLILILGSMSMLPVLGQTSAPKYSNEFLSIGISARALGMSN
                     SVVASVNDVTGGYWNPAALTLIESDIQLGVMHSEYFAGIAKYDYGSFAMALPEKNAAV
                     GLSIIRFGVDDIPNTLYLIEPDGSINYDNITAFSVADYAFLGSYAQKLNWHNLRVGGN
                     IKVVHREAGDFATAWGFGLDAGAQMDLNNWKFGATFKDITSTFNAWSFSFTEEEKQVL
                     LATGNIIPENSLEITTPKLILGAAYDFTLNENFGLLAEVNADITTDGRRNVLISADPL
                     SIDPHLGLEADYKEFIYVRAGIGNVQRYIPDDLFEEAWTMQPNIGLGVEIGPVMLDYA
                     YTNIGKQSEVLYSHVFSLLFNINKTPKPAAE"
     gene            complement(9855..10529)
                     /locus_tag="PLB46_01615"
     CDS             complement(9855..10529)
                     /locus_tag="PLB46_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75237.1"
                     /translation="MNFLSHFYHELPVSDPNFAAGLILPDILSNYSYRTGEVVKLHPQ
                     KLVYTDNPELSRMTEGVKRHYFVDGHFHESAFFEQNTTAISALIKATGFTCFDKRLYA
                     ISHVLLEVVLDRTLLTSRPEVCKQLYDLIEMVDTSQISRLVAINTQAMDPDGIGNHFK
                     VFRERKFLYDYAYDERLTGIINGINIRLGNPPMNETDQKRFKSVIHDSEKTILSQKFP
                     KFPTDS"
     gene            10629..11909
                     /gene="tyrS"
                     /locus_tag="PLB46_01620"
     CDS             10629..11909
                     /gene="tyrS"
                     /locus_tag="PLB46_01620"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRF75238.1"
                     /translation="MKNFVEELRWRGMLFDMMPGTEEFLNKGMAKGYIGFDPTADSLG
                     VGNMVQIMTLLHFQNCGHLPIALIGGATGMVGDPSGKSAERNLLDEAALQHNIQSQQK
                     QLAKFLNFNSGENAAQLVNNYDWMKNFSFLEFLREVGKHLTISYMMAKDSVQTRMEKG
                     ISFTEFSYQLLQGYDFYYLWKNNNVMLQMGGSDQWGNITTGNELIRRKGSGEAYAICT
                     PLIKKADGTKFGKTEGGNIWLDPKRTSPYRFYQFWLNASDADAETYIKIFTLFERETI
                     EDLITRHRAEPHLRLLQKELAKDITTRVHSEEDYHNAVAASDILFGKATQADFEKLSD
                     ELLLDVLEGVPQFVVDKTIFSTGIGFADLLTEKSNILASKGEYKRAMQENSLSLNKEK
                     VSDAEKIITADLLINGKYLLIQRGKKNYFLIIAQ"
     gene            complement(11896..12486)
                     /locus_tag="PLB46_01625"
     CDS             complement(11896..12486)
                     /locus_tag="PLB46_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75239.1"
                     /translation="MKSILLILFFAVTTSASAQSLFKDLTVVQFGDSMLINWTLTAGS
                     TCFDMHLKRAGKDTEFEDIFTVPGVCGGASDQYYDFVDNQNLVSGTTYQYFISASNGV
                     YASETVEILYVNAGDMQLFVYPNPTQTAIQVTVDNTFTPSFFVELYTVDGKLIQQSKE
                     VNNLFTINTIHLLPSSYLLKITTEDGDVLAQQFIVQ"
     gene            complement(12606..13583)
                     /locus_tag="PLB46_01630"
                     /pseudo
     CDS             complement(12606..13583)
                     /locus_tag="PLB46_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
     gene            complement(13910..15178)
                     /locus_tag="PLB46_01635"
     CDS             complement(13910..15178)
                     /locus_tag="PLB46_01635"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRF75240.1"
                     /translation="MNVDVLLGLQWGDEGKGKVVDYLAEQYDVVARFQGGPNAGHTLI
                     LNNGVKFVLHTIPSGIFLENCLNLVGNGVVIDPITFRMEINKLAGAGVQVNNRLVISR
                     KAHLILPTHKMLDAASEASKGADKIGSTLKGIGPAYMDKTGRNGLRVGDIESPDFKEK
                     YNKLKEKHFQLLKNYGDVPSEVPNEQEWMEAIDFLKQITFVDAEYLINDNIKSGKRIL
                     AEGAQGSMLDVDHGTYPFVTSSNTITAGVCTGLGVAPQKIKEVIGISKAYCTRVGSGP
                     FPTELFDETGEELRKIGNEFGATTGRPRRCGWIDLPALKYTIMINGVTQIAITKIDVL
                     NTFAEIKACVGYKINGVVTDQMPYDIVDAKIEPVYETFKGWECNLDHCKTKADLPQEL
                     LNYLQFLEWELKTKISMLSAGPERDKLIIM"
     gene            complement(15352..15705)
                     /locus_tag="PLB46_01640"
     CDS             complement(15352..15705)
                     /locus_tag="PLB46_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRF75241.1"
                     /translation="MEYHIEKKNENTLVVKLNGRLVGEYQTVQVAEQLEEDIEDGFTN
                     IIFDFSELEFINSSGLNFLLKILTKVRRVDGEVVLCAMKDHLKTLMVTTKLTSFFTIS
                     DSVTDALGNFEPEKV"
     gene            complement(15721..16209)
                     /locus_tag="PLB46_01645"
     CDS             complement(15721..16209)
                     /locus_tag="PLB46_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRF75242.1"
                     /translation="MEQQIKHLEEIKNIFAAHLETKGLRKTPERFAILEEIYSRTDHF
                     DAESLYSHMLKKEYHVSRATVYNTLELLLTCDLITKHQFGKNLAQYEKSYGYKQHDHI
                     ICVDCKKVVEFCDPRIHQIQTMMGDLLNFKITHHSLNMYGICGNCQKIREMNEQKAEK
                     TA"
     gene            complement(16220..18427)
                     /locus_tag="PLB46_01650"
     CDS             complement(16220..18427)
                     /locus_tag="PLB46_01650"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRF75243.1"
                     /translation="MEAAEPLDLEKEKKLILKEYKNLLRGLRNNMTGAERKQIRLAFE
                     MAVDAHKNMRRKSGEPYIIHPLRVAQIVVNEMGLGATAAICALLHDVVEDTELTLDDI
                     KREFGIKVSRIIDGLTKVSGVFDLTSSIQAENFKKLLLTLADDVRVILIKIADRLHNM
                     RTMDSMPRKKQLKIASETLYLYAPLAHRLGFYAIKSELEDLGMKYQETEAYKDSAKRL
                     AETKRERTRYMNEFMKPLRERLEKDGFKFEMYGRPKSIYSIYNKIKNKGVTFDEVYDL
                     FAIRIILETPVDHEKADCWRVYSMVTELYHPNPDRLRDWISTPKANGYESLHTTVMGP
                     KGKWVEVQIRTRRMDEIAEKGFAAHWKYKEGSNDKALDTWLREIRELLRNPEFNALEF
                     LDDFRLNLFSKEIYVFTPKGDLKILPQGATALDFAFEIHTDVGYHCIGAKVNHRLIPI
                     SHMLNNGDQVEIITSKKQKPNEDWLNFLITSRARSKVKNALKEEKRAVAMEGKTVLEK
                     RLKNMKVEFFDKNIQLLMEYYHQPTSMELYYRISTKDITLKELKDFTVQGGKLIPPVV
                     DKEHSVDYERLEKIATSNAELLIFDNAMDNIKYALAKCCNPIVGDDVFGFITINEGIK
                     IHKTNCPNATELMSKYGYRIVKTRWTQQKDIAFLTGIKVTGLDDVGLVNKVTNIISGD
                     MKLNMQSLVMDAKDGLFEGTIKTFVKNTEQLETLLRKLRSMKGVISVTRMETT"
BASE COUNT         5701 a         3640 c         3262 g         6042 t
ORIGIN      
        1 aaatagggca tcatagcctc tatcactttg ggttccagct gcgtggtagc agcattgtct
       61 aggtaaacgc gcatgtaatt cagttaaacc gcaaaattaa gcaattaccc cgagtagctg
      121 aaaaaggccc tccaaatgac tatttcatga catttttgat gtcatccata atctttttgt
      181 tgatgttctc agccgtggtt ttggtagaac tttcggagta gatgcgaata ataggttcgg
      241 tattgctggc gcgaagatgt acccagtcgc cacccatttc tatttttagc ccgtccgttt
      301 tattgagttt ttccttctta tatttttcct ccattttgcg caaaatatat tgcacatcgg
      361 ttccttcagg caattcgatt ttattttttg ccataaagta gtttggataa tcggcgcgta
      421 agcgtgaaca acttttacct gaagttgcca aatgcgacaa aaacaaggct atgcctacta
      481 atgcatcgcg accataatgt aattcaggta aaataattcc tccattacct tctccgccaa
      541 taatggcatt tacttctttc atcatgttca caacatgcac ttcccctact gcactttgat
      601 gatgttctac tttatgtttt gcggcaacat cgcgtaaagc ttgggttgat gataaattcg
      661 aaacaacatt tccgggcgta tgtttgagga cataatcggc aatagaaact aaagtatatt
      721 cttcaccaaa catatcgccg ttttcgcaca caattgccaa acgatcaaca tcagggtcta
      781 ccactaatcc gacatccgct ttttggtgtt taacctcatg cgcaatagtc cctaaatgtt
      841 caggcaatgg ttcaggatta tgcgggaaac gtccatttgg attggtaaat aattccacca
      901 catcggtaac accaagtgct accaataatt caggaacgga aatacctccg gtagaattca
      961 cacaatcgat aaccacttta aaattgcgtt ctttaattgc ttttttattt actaatggca
     1021 attccagaat tttatcaata tgtttttgga tataataaaa tttgtgttgg tattcaccta
     1081 atttatctac atcactgtaa actatttctt cactgttggc aattgctaaa accgcttcac
     1141 catcagcacc tgaaataaat tcgcctaaat gatttaataa tttcagtgca ttataatttt
     1201 ttggattatg tgaagccgta ataataatac ctccacctgc ctgttcacga ggaaccgcaa
     1261 tttcaactgt tggtgtagta gataatccta agtcaatgac tttaatacca attgattgta
     1321 atgtaccggt aaccagatga tttaccattt caccactgat acgcgcatca cggccaatca
     1381 caattgttgg atttccggtt gtatttttta tccaggtacc aaaagccgca gtaaatttga
     1441 taatatcctc aggggttaga ttttgtccgg ccttccctcc tatagttcct ctaataccgg
     1501 agattgattt tattagtgtc atattcgaaa tacaaagtta tacaaattca aatgatgcga
     1561 tataacgaat aaatgtttaa atcttgtact tttgttcatt atttaaaata tggctgtcac
     1621 aaaaaactgg tttgaaactt ggttcagtca aggttattac gacatactgt atcagcatcg
     1681 tgatgaagac gaggctgccg gctttttgga tgcattgatg gaaaaactac agccaccaac
     1741 aaacgccaca atattggatg cagcctgcgg taaagggcgc cacgccatct atttggcaga
     1801 aaaaggctgg gatgtcacag gaatcgattt atcttacaat tctattaaag aagcaaaatt
     1861 ggcagaaaat gaacatttat cattttttca gcacgatatg cgtaatgtgt tgcgtgtaaa
     1921 ttattttgat ttcattttta atttttacac cagctttgga tactttgcta acattaaaga
     1981 tgatatcaaa tgtattcgtt catttgcgaa tggattaaaa cccggaggta aactggttat
     2041 cgatttttta aatgtattta ccaccttaca aaatttagtg gaagatgaac ataaagtgtt
     2101 tggtgatatt caattcgata ttacaaaaag ttatgcgaac ggaatgatat ctaaaaaaat
     2161 tgaagtaaaa cataagaatc aacatcatac ttatcacgaa catgttcgtg cactacaatt
     2221 acaacatttt gatcactatt gctcattggc aggattaaaa attgtagcta cttttggcga
     2281 ttatgatttg cagccatata ataaattaca atctgaacga cttattatca tagcagaaaa
     2341 atgatatttc cggaatttat acaacaattt gacgaatggg cttttttact tatcaataat
     2401 aagtggtcaa atcatgtatt tgatatcata atgccaccag ttcgcgataa aaatttctgg
     2461 ataccactgt atattattat tgctattgtt ttagtatatc aatatcgttg gaaaggactt
     2521 acgcttattt tattgttggg tctcaatttc ggtgttagtg accaattgag cagtgctgtg
     2581 ataaaaccgg cagttggccg cgttcgcccg tgtaatgacg aatcctttaa agaacaggtt
     2641 accctgcgaa ttgaaagatg tggtgcaggc aaaagtttta cctccagcca tgctaccaac
     2701 acctttgcat ttgcaataat gttaacactg ctgttccgaa aaaaatatag atggattgcc
     2761 cctgtagctt tgttttgggc gagtttggtg tcgtatgcac aaatttatgt gggtgttcat
     2821 taccctatcg acattttagg tggtgcatta ttaggtacgg gaattagctt aattatctat
     2881 aaaattgcat cgcgttacgt gctacccaaa cttggtccgg aactttaaag caggcaaatc
     2941 agtattataa cacatgagca tacttcaaat tgtattcatt atttcagctt cagttgcagg
     3001 tattttagct gcctggttcc tgcgcaaaca aacacatagt gagttactca gtattttatt
     3061 atcctttagc ggtgcctatt tattgggtaa tgttgtgctc catttattac cagaaatttt
     3121 tgcggatgct catgccggac accaggaaac tggtgcacat gcacatgact atactgcagc
     3181 tatctatatt ttgcttggtt tttttataca attgcttatt gtgcagttta ccagaggtat
     3241 cgaacacggg catttacatt tacacgaaca tcaaagtaaa ggatatattg caggcgtatt
     3301 tttcggattg agttttcatg cctttatgga aggtgttccg ctggttggta acaatatgga
     3361 agccgctcaa atgcaaccac tttttaatgc cattttagta cataaaattc ctgaggcatt
     3421 tgcgttagca actgtgttat ttttttctgt aagtaaacaa ttaaatgcgt atttattatt
     3481 ggcatttttt gcattaatga caccattagg aagttatgcc ggtaattacc tcactaccca
     3541 acatgtgatg gattttaaat ggctgatagc aatagtttgc ggttcgttaa ttcacatcag
     3601 tactaccatc atttttgaat ccagcggcaa agcgcataaa attagtatgt ataaatttat
     3661 tgccatttta gccggagcag gaattgcact tattgctgct atgatggaat aatgtttatc
     3721 tcgctaaata catttttcca aaacctgaat taaagtattg atcagttccg gtatcatatt
     3781 tttcaatcga ttcaccgtgc agacgggtta ccactctata cacatataat ccatttgcta
     3841 atgggtcacc aaactggtcg gtaccatccc aggcaaattc ggtgatatta tttcctatat
     3901 gtagttcacc taattcgctg cgcataattt cgcggactac ttttccggtg acagtcataa
     3961 tttgaatttt gaaatattcc ggcacttcac ttccggttaa ggtaaataca aatcgggttt
     4021 gtgaggtgaa tggatttggg taattcatta cattactcac cattgcctta ttgattactt
     4081 caaaagaaat agtataatcg atatcacctg ccgtatttcc ggtgttatcc attccctgca
     4141 cacgtaattc atacgtgcca tccatactaa aaatcggatt aatttctacc cttgcactat
     4201 tgttttcagt taaattagaa gggtcagccg gataaaattt ggtggttacc ccatcgtaat
     4261 aataatcgtg gaaactgtaa tttggatatt gaaaggtaat tttcaacaac gaggtatcag
     4321 caagagccag ataaggattt tcgtctttca aattaataat aatttgtggc ttagcactca
     4381 caatatctcc atctaaaata tgaactccgt caaaagttac atctaataat ggatctttag
     4441 catcaccaat aacttgcaca ggcaaatagg cgaaattatt gtaatgaaat tgttcttcct
     4501 gatcattttt agggttcact tcggctacca gtgtcgaccg cccaattgga atttgattgg
     4561 tattaaattg aatagtcgca atcatagatt catcacttag caaactatct tgtcgcgaat
     4621 acggtacctc gtggtaaaaa ttattggcat caaaaatgcc aaaattaatt aacaagctat
     4681 ccatattgta atcacttaca ttcgtaatag ccaattctaa acttccaggt gcaccttgtt
     4741 gtagtgtatc attagtaaaa gcaaagtgta agtttgggtt aacagcagct tctggaactg
     4801 ggtcataaag aacacgccaa taattaaatt gagcaggtgt tcttaacgta tcatcttttg
     4861 tttctaaacg caatttaata taaggatata aatctgcatc agcaaatgta atagaagtat
     4921 ctccactttg taaaccggtt tttaaaacgg tttctacgcc attaattttc acgccaataa
     4981 ccgatatact gttagtatct acgccaggta aatcgttcgc ataaaaactc cattgtgctt
     5041 tttcccattc ttcagccggc ccaatcaaag gcgtttcaat atttccttta ttccaattcc
     5101 cgttaattac aaatgttgct tcaattttat gcccttcagg atcgccaatt atttcaactt
     5161 tagaatctgt atttcctttt tgcgcataaa aaatatagga tctgtcataa tcaaaaacag
     5221 acaatgcatt aatatctgta gcaccatagg cattaaatgc atcgaataaa ctatgtccat
     5281 aaatcaaagt atcatctaac catgcataaa aatccgggta attgttgcta taacaaataa
     5341 aatgcgtgct atcaggcaat tcattcatca tgaaattgta taatttctcc ctggattcag
     5401 gtgagttggt ataaaattga ataacccttt catatgcatc cgcgctgcaa taaacatcac
     5461 catatggacc taaattagtt gcccccactt cacttgtttg ccaaagttcg gccgaattgg
     5521 catcagctac taataccaca aatcccgtag acgcgcatga cccgattgcg agcaattcac
     5581 catccgcata actggttact tctgtccaat gtgttgttgg gtaaatacct gttgcaactt
     5641 cgtatgtttt tacatcagga acaaaagtaa attcacgtga tggttcgaga tttacatttg
     5701 taaactcatc ttccaaatat tgataataat gcgattgatt ccaacctgtt atttcacctg
     5761 gtaaatataa aaatgaacta gaacgccaca tggcttcaga tccatctatg gtatcaggag
     5821 ttatacgcca ataatatact acatttggta tataggtcaa tgggggattt tcccatttaa
     5881 ttacaccacc tgccgatgtt accattgttg tttttcgcaa tggtgaatta aacaatgttg
     5941 ttgtgtctgc ttcgaaaata aattgtttaa caggagcaaa agcatcagtg gatgaagctg
     6001 caaaatattc cggcacatga ttcattatgg taaattccac cggataaatt ggcatcgcat
     6061 catcagaaat tacaaataaa gtggtattta aaatattatt gaattcatct atttcgccaa
     6121 tttcattttc tttgtccaga tgaattgcaa aattattcaa tcctgcacct gctaaaccat
     6181 cagtagcaaa tggaattatc aaagtatctc taaaacgagt tgccggaaac cgttcaaaaa
     6241 aagtagatgt ttcccctcca aactttgtcc gtttcacctc aacgaaataa ctggaatcaa
     6301 ttgcagatcc caagttggtt acgattaaat gcatataaat actatctgtt ccggcagaaa
     6361 ttacgtccgg ttcaaaatgc acatatggcg cttcaattgc ataatcaggt ttgtcgtgcg
     6421 tgtttaaacg tatagcagga tcaccttgca aagtattatg ctgaattgtc atcctgtcaa
     6481 atggattgtc tgtcggccac aatgtatctg ctacagcgcg cattgcaata cccattggca
     6541 gattatagga attactcgca agttcacgat aaaataaagt agcataagta tgcaaattgg
     6601 ctgcaataga gaaattactt gcagctaaaa atgcaatagc agctttattt tcagcaaaaa
     6661 tatattgttc acctaaggta taagcatcgc cgtgaatagc agcagtattg caaccatttc
     6721 catataccgc aaaatattta ccttcattat taaactcgtt gggggagccg atattataat
     6781 caaaactacc ggttgatgaa tgtccgaaaa aagtaattaa tgatacaccg gtattaatta
     6841 aagaatcaac ataagccgat tctgaataaa aaatcggatc cgcatttaat ttattaaatg
     6901 gaattacatt tcctccatac aaagtgtctt caataatgtt tttatattga ttcaaaaacg
     6961 aattaaataa ggtttgctca aattcagaag cgccacctgc aaaatgcaaa acgtttttca
     7021 tccaggcttt attagctatc gtttgggtat ggtctgcctg ttgtgcttca aattcaatta
     7081 ttttatcata ataaatacgg acatcatttg gcgtttttgc agatattcta cccactgcaa
     7141 ctaacggcac aaaatcacct gttcgcgtta ctaacatatt atcagacccg ggattaccaa
     7201 atgttggcac caaactcgca gcatattcag gtgttccatt agcacgtgtt acatcataac
     7261 tgtatgactt acctattaaa aaaagaaact caggagctgt tacgaaatta tctactgcat
     7321 acgtcacaaa attgcgaatg gccaatggat gttttttcag cccataagaa aacatgtcat
     7381 ataaaacatc aatatcaata acaatagcat cataactacc tcctgcaata cttgcccgat
     7441 aatctctata agcgttaacc caattagttc catctacatc ttcaaacaat actttattgg
     7501 aaataatcat ataattaccc tgattggcgg tggctccata gttgacaaaa gtgacgggat
     7561 ttaatttagg tactgaaaat atatcagtgg catcctgact gctaaaatat aattctgcat
     7621 tagtggcatt atagtctaaa tgaaattttg aaatatctgc ttcaacaatt gcttcaatac
     7681 gtttgtgatt cgaaaggtcg tataaaatgg gtgaggtaaa tttttcatta aaattagtac
     7741 attccaaata ggttgttgct ccaacaactt gtgtcaacca tgtttttaat accgattcat
     7801 tgttacaact aaaagacctt ggataattga catcaataaa tgaatatcgc tggtaatcgg
     7861 tagaaactgg cccacttgaa aaagtaatgg tattcgcagc taatatatct gctaaattaa
     7921 attcataacg accaactctg taattaaaaa aagtggtgtc ctttattatt tctccattcc
     7981 atttaatcaa aaagtggtgc tccccatccc cgttggcaat aagcgccgtt tttaatctgg
     8041 ggataaaaga aggaccggca taaaaatcag gtgtatttac agtaacatta aaattactaa
     8101 ggttgtattt gccggtggca tacccttcac cttctgtcat gtcagaagat aaaatatcat
     8161 taaaaggatc acctatgtta aaatttgatg aaccataacc cgcaccgtat acaatgcgcg
     8221 tatgataatc gtgatatagt aatggtgcag gtgtgtccgt taaatcgtta ataactacct
     8281 caagacgatg gttagtaatt aaatcgttcc aggtcaaaaa atacatggca gtatctgtta
     8341 ttaaactctc gtattgatgt aactgatact ccggaaattc aaacattaat gaatcgaaag
     8401 taccgtcatt tttttcaccg taaaactcga tataatccgc tgcgttaaaa acaccatctc
     8461 cttcacctga tatatgaata tattgctcaa ctcctttatt tattaactga aaattacgcg
     8521 gatcaactgt ggcaagcgga acaccaatat cgttcatagc tgaattgagc acatcgtaag
     8581 aaatacgata tataccattt ttagcaatag ggaattttaa atattgctgg tcgtactcaa
     8641 tccattcatt atggtaagtt tgagcattta cctgtaaaat tgataatatg aaaaaaaata
     8701 agagtaaatg ttttttcata cagttggttt tgtgtgcatg tttaacggtt tattcagctg
     8761 ccggcttcgg ggttttattg atattaaaca acaaactgaa tacatgagaa tataatactt
     8821 cgctttgttt gccaatattg gtataggcgt aatccagcat taccggacca atttctacac
     8881 ctaatccaat attaggttgc atagtccatg cctcttcaaa taaatcatcg ggaatatatc
     8941 gttgcacatt tcctattccg gcgcgcacat atataaattc tttgtaatct gcttctagac
     9001 ccaaatgcgg atcaatactt aaagggtcag cactgattaa aacatttctg cgaccgtcag
     9061 ttgtaatatc tgcattaact tcagcaagca atccgaaatt ttcgtttaat gtaaaatcat
     9121 acgctgcacc taaaattaat tttggtgtgg taatttctaa actattttca ggaattatat
     9181 ttccggtagc caataacact tgtttttctt cttctgtaaa tgaaaaactc caggcattaa
     9241 atgttgaagt tatatcttta aatgttgcgc cgaatttcca attattaagg tccatttgag
     9301 cacctgcatc taaaccaaat ccccaggcag ttgcaaaatc tcctgcttcg cgatgtacta
     9361 ccttgatatt tccaccaact cgtaaattat gccagttcag tttttgcgca taggaaccta
     9421 aaaaggcata atcagcaact gaaaatgccg tgatgttgtc gtaattaata ctgccatccg
     9481 gttcaattaa atacaaggta ttcggaatat catctacacc gaagcgaata attgataatc
     9541 ccaccgcagc atttttttca ggcaatgcca tggcaaatga accgtaatcg tatttagcta
     9601 taccggcaaa atattcgcta tgcataacgc ctaactgaat atcagactca attaacgtaa
     9661 gtgcagccgg attccaataa ccgccggtta catcatttac cgaggctact accgaattac
     9721 tcatacccaa agcacgagca gaaataccta tagatagaaa ttcattgctg tatttgggag
     9781 cagaggtttg acccaaaaca ggtagcatag acatactgcc taaaatcaat attaaatact
     9841 ttctcatgta tggttcacga atctgtcgga aatttaggaa atttttggct gagtatcgtc
     9901 ttttcgctgt catggattac acttttaaac cgtttttggt cagtttcgtt cattggtgga
     9961 ttacctaaac gaatatttat cccgtttatg atgcctgtga ggcgctcatc ataagcataa
    10021 tcgtacaaaa acttccgctc cctgaaaacc ttgaaatggt tgccaatacc atcgggatcc
    10081 atggcttggg tgttgatggc tactaagcgg ctaatctggc tcgtatcaac catttcaatg
    10141 aggtcgtaaa gctgcttgca aacctcaggc ctgctcgtta gcagggtccg gtcgagcact
    10201 acttccagca aaacatggct tatggcatat aaccgtttat cgaaacaggt aaacccggtt
    10261 gccttgatga gtgcagaaat tgcagtggta ttttgctcaa aaaaggctga ttcatgaaaa
    10321 tgtccgtcca caaaataatg ccgttttacc ccttccgtca tcctgcttaa ttccggattg
    10381 tctgtgtaca caagcttttg aggatgtaac ttcaccacct ccccggtgcg gtaactataa
    10441 ttcgacaaaa tatcaggcaa aatgaggcct gcagcgaaat tggggtcgct taccgggagc
    10501 tcgtgataaa agtgggacaa aaaattcaaa gcctgatggt ttattgtaca atatacgaaa
    10561 ttaagtaacg aaaagttgag gaaagggttt acatctgcat ataattttta tgtcattatt
    10621 tttgcagcat gaaaaatttc gttgaagaat tacgctggag agggatgtta ttcgatatga
    10681 tgcccggaac tgaagagttt ttgaataaag gcatggctaa aggctatatc ggttttgacc
    10741 ctactgccga ttcgcttggc gtgggtaata tggtgcaaat tatgacgctt ttgcactttc
    10801 aaaattgcgg acatctgcca attgcactca ttggcggtgc aacaggaatg gtgggcgatc
    10861 cttcgggtaa aagtgccgaa cgaaatttat tggacgaagc agccttgcaa cacaatatcc
    10921 agtcacaaca aaaacaattg gcgaaatttt taaattttaa ttccggagaa aatgctgctc
    10981 aactggtaaa taattacgat tggatgaaaa acttttcatt cctggaattt ttacgggaag
    11041 tgggaaaaca tttaaccatt agttacatga tggctaaaga ttccgttcaa actaggatgg
    11101 aaaagggtat ttcatttacc gaattttctt atcagttatt acagggttat gatttttatt
    11161 atttatggaa aaataataat gtgatgttac aaatgggtgg tagtgaccaa tggggaaata
    11221 ttactaccgg caatgaatta attcgacgca aaggaagtgg tgaagcttat gccatttgta
    11281 caccattaat taaaaaagct gacgggacaa agttcggtaa aacagaaggc ggaaatattt
    11341 ggttagaccc aaaacgtaca tcgccttatc gcttttatca gttttggtta aatgcaagtg
    11401 atgctgatgc cgaaacttat attaaaatat ttacactttt tgagcgtgaa actattgagg
    11461 atttgattac acgccatcgc gcagaacccc atctgagact gcttcagaag gaattagcaa
    11521 aagatattac cacacgtgtc cacagtgaag aggactacca taatgcggtg gcagcaagtg
    11581 atattttatt tggtaaagca acacaggccg atttcgaaaa attaagtgat gaattgttgt
    11641 tggatgtact cgaaggtgtc cctcaatttg ttgtcgataa aactattttt tcaacgggaa
    11701 ttgggtttgc agatttgctt acagaaaaaa gtaatattct cgcctctaaa ggtgaataca
    11761 aacgcgccat gcaggaaaat tcgcttagtc taaataaaga aaaagtgagc gatgccgaaa
    11821 aaataattac cgccgattta ttaatcaacg gtaaatattt attaatacaa cgcggaaaaa
    11881 agaattattt tttaattatt gcacaataaa ttgttgtgcc agcacatcac cgtcttcagt
    11941 agtaattttt aataaataac tgctgggtaa taagtggatg gtgttgatgg tgaataaatt
    12001 atttacttct tttgattgct gtattagttt accatctaca gtatacaact caacaaaaaa
    12061 ggaaggggta aaggtattat ctaccgttac ctgaatggca gtttgcgttg ggttcgggta
    12121 aacaaataat tgcatatctc cggcatttac ataaagtatc tctaccgttt cactggcata
    12181 aacgccattc gaggcagaaa taaaatattg ataggtagtg cctgaaacca ggttttggtt
    12241 atcgacaaaa tcataatatt ggtcggaagc tccaccgcaa accccgggta ccgtaaatat
    12301 atcttcaaat tctgtgtcct tacctgcgcg tttcaaatgc atgtcgaaac atgttgaacc
    12361 ggctgtcagc gtccagttta tcagcatact atcgccaaat tggactaccg ttaaatcttt
    12421 aaacaaactc tgggctgaag cagatgtggt aactgcaaaa aatagtatta ggagtatgct
    12481 tttcatattc ggcttaataa cgaaatttct tccggcttag tatatagtaa cacaatttcc
    12541 gattaagatg gcgataccaa tatgcaatag gatgcatcct taaaccttat ccagtaatgc
    12601 agttattact cggcatcacc atataacacc tgccgcagcg cctgcaattt taaataacac
    12661 tccgccaatt cctgctccgg aactgagtca tatacaatag cacctccggt ctgaatactg
    12721 agatgttgat tttctgcatt gtattgaata cttcgaataa ccacattaaa atcaacatca
    12781 ttatcaggtg taaaatatcc aaatgcaccg gaaaataatc cgcgtttgct tacttcatat
    12841 tgttcaatca atagcatggc cattacctta ggtgcacctg tcatgctacc catcgggaat
    12901 gcatctttta tgacatcaac tacatttacc tcttcactaa tttgtgcact tatagtagat
    12961 accatttgat gtacttgggg aaaagaataa ataccaaata actcttctac ctgaatactt
    13021 ccggttatgg catgtttagt taaatcgttg cggaccaaat caactatcat cacattttct
    13081 gcgcggtctt ttatactgtg gtataaatca cttttttatt gtttatcttc aaacgcattt
    13141 ttttcgcgtt tgtgggtgcc tttaatgggc tgactaataa ttttattccc atattttttc
    13201 agaaaccgtt cagggcttgc tgaaattaaa taacgatgtt gccatttaaa taatacagca
    13261 aatggagcag cacttaatgt acataattta tcaaaaactg cagatgcatc tagtgtgcca
    13321 ttggtataaa attcctgaca aaaattgagt tcgtaaatat caccttctgt tatatggtga
    13381 ataatatctt ttactttttg caaatatctt tccgccggaa tccgcggctg taaaggtgct
    13441 aaggctgacg gaacagattg tgaggttgct tcatttattt gtcgaaatat tttttcaaac
    13501 gaactagcat tttcttccaa caaaaaaatg gtacaggtat tttcttttac ggttatataa
    13561 gtttcaggaa caaaaaatac catttcaggc caaccaatac catcaaagtt tttggacaat
    13621 aattgttcag tttcctgttt taaatcatac ccgaaaaatc caaaacaata atctttaaat
    13681 gtttcccgaa aaaaacgaag ctgttcaaat gcatctcctg cattggttac caattgttgg
    13741 tatgctccgg ctgctaaggc atagtcgaaa gtgacaggtc catacaccgt atttttacag
    13801 ctatctaaaa atgcgaagca actaaaatgc tgtgcataag ctgataattt tttgcggaag
    13861 tcatcagtat cagcaatggt aaatgtttta ctgatgcgcg tcataataat tacatgataa
    13921 ttaacttatc cctttccggt ccggcagata acatggaaat ttttgttttt aattcccatt
    13981 ccaaaaactg cagatagtta agtaattcct gaggtaaatc tgcttttgtt ttgcaatggt
    14041 ctaaattaca ttcccagccc ttaaatgttt catacaccgg ttcaattttg gcgtctacaa
    14101 tatcataagg catttggtca gtaacaacac catttatttt atatccaaca catgctttta
    14161 tttcagcaaa tgtatttaat acgtcaattt ttgtgatggc aatttgtgtt acaccattaa
    14221 tcataatggt atattttaac gcaggtaaat caatccatcc gcaacgacga ggtcgacctg
    14281 tagttgcacc aaattcgttg ccaattttgc gcaactcctc tcctgtttcg tcaaataatt
    14341 ctgttgggaa cggacctgaa ccaacacgcg tacaataggc ttttgaaata ccaattactt
    14401 cttttatttt ttgcggtgct acacctaatc ctgtgcacac accggcagta atggtattgc
    14461 tggaagtaac aaaaggataa gtaccatggt ccacatctaa catagagccc tgagcacctt
    14521 cagctaaaat gcgtttaccg gatttaatat tatcgttaat taaatattca gcatctacaa
    14581 aagtgatttg tttcaggaag tcaattgctt ccatccattc ttgttcgtta ggaacttcag
    14641 atggcacatc gccataattt tttaacaact gaaaatgttt ttctttcagt ttattatatt
    14701 tttctttaaa atcaggtgat tcaatatcgc ctacacgtaa tccgtttcgc cctgttttat
    14761 ccatatatgc aggaccaatt ccttttaatg tggaaccaat tttatctgca ccttttgatg
    14821 cctcacttgc tgcatccaac attttatgtg taggtaaaat taaatgtgcc ttgcgggaaa
    14881 ttaccaaacg attatttacc tgaacaccgg ctccggccaa tttattaatt tccatacgga
    14941 aagtgatagg gtcaattaca acaccgttgc caaccaaatt cagacaattt tcgagaaaaa
    15001 taccggaagg aattgtgtgc aaaacgaact tcacaccatt attgagaata agtgtatgtc
    15061 ctgcattcgg gccgccctga aaacgagcta ccacatcgta ttgttctgct aaataatcaa
    15121 ctactttacc tttgccctcg tcgccccatt gaaggcctaa taaaacgtct acgttcataa
    15181 catggattaa aatgcgaaat tataccacag gcttaccttc agcaaggtac attccccttt
    15241 tttttcaaga tattgttaac agttatgagg tgaccggaaa agtaaggttg gtcaaaaatg
    15301 gtggacctta cttttgaaaa acaggtgaaa attgttgtaa gtgattgatt atcaaacctt
    15361 ttcaggctcg aaattgccca aggcatcagt tactgaatcg ctaatggtga aaaaactggt
    15421 gagcttggtg gtcaccatca atgttttgag atggtctttc atggcacaga gcactacttc
    15481 tccatcaacc ctgcgcactt tagtcagaat tttgagcagg aagttcaatc cactgctatt
    15541 gataaactcc agctcgctga agtcaaaaat aatattggtg aacccatcct caatatcttc
    15601 ttctaactgc tcggccactt gcacggtttg atactctcct accaaacgcc cgttcaactt
    15661 caccaccagg gtgttttcat ttttcttttc tatatgatat tccatcgtgt tacaggggta
    15721 ttaagctgtt ttttctgctt tttgttcatt catttcacga attttctgac aattaccgca
    15781 aatgccatac atatttaatg aatggtgggt gattttaaaa tttaataaat cgcccatcat
    15841 ggtttgaatt tgatggatac gcggatcaca aaattccact acctttttac aatcaacgca
    15901 aataatatgg tcatgttgtt tataaccata tgatttttcg tattgcgcaa ggtttttccc
    15961 aaattgatgt ttcgtaatta aatcgcaggt taataataat tcgagtgtat tatacaccgt
    16021 ggcgcgactc acatgatact cttttttcag catgtgtgag tataaacttt ctgcgtcgaa
    16081 atggtcggtg cgactataaa tttcttccag aatagcaaaa cgttcgggag ttttacgcaa
    16141 gccttttgtt tctaagtgcg ctgcaaaaat attttttatt tcttccagat gttttatttg
    16201 ctgttccata atcttgtttt taagtagttt ccattcttgt tactgaaatc acacctttca
    16261 ttgagcgtaa ttttcgcaac aaggtttcta attgttcggt attttttaca aaggttttaa
    16321 tggtgccttc aaacaaaccg tccttagcat ccatcaccaa actttgcata tttaatttca
    16381 tatcgccgga aataatattg gtaaccttat ttaccaaccc aacatcgtct aatcctgtaa
    16441 cttttattcc ggttaaaaag gcaatatctt tttgttgtgt ccaacgtgtt tttacaatgc
    16501 gataaccgta tttactcatt aattcggtag cattcggaca attggttttg tgaattttga
    16561 tgccttcatt aatggtaata aatccgaata catcatcacc aacaattgga ttacaacatt
    16621 tggccaatgc atatttgata ttatccatgg cattatcaaa tattaataac tcagcattcg
    16681 aggttgcaat tttttcgagg cgctcataat ccacgctgtg ttctttatcc accacaggcg
    16741 gaattaattt ccctccctgc actgtaaaat ctttcagctc ttttagtgta atatctttgg
    16801 ttgaaatacg ataatataat tccatactcg ttggctgatg ataatattcc atcagcaact
    16861 gaatattttt atcgaaaaat tcgaccttca tatttttcag ccgtttttct aatactgttt
    16921 taccttccat ggctactgca cgtttttctt ctttcagtgc attttttact ttacttcttg
    16981 cacgcgaagt aattaaaaaa ttaagccagt cctcgttcgg tttttgtttt ttagaagtaa
    17041 taatttctac ctggtcgcca ttgttcaaca tatgactgat tggtatcaag cgatgattta
    17101 ctttcgcccc aatacaatgg tatccaacat ccgtgtgaat ttcaaaagca aagtcgagtg
    17161 ctgttgctcc ctgaggtaat atttttaaat cacctttcgg agtgaacaca tatatttctt
    17221 ttgaaaataa atttaatctg aaatcatcca aaaattcgag tgcattaaat tcagggttac
    17281 gcaacaactc acgaatttct cttaaccagg tatctaatgc tttatcgttt gaaccttctt
    17341 tatatttcca gtgcgcagca aatccttttt ctgcaatttc atccatgcgt cgtgtacgaa
    17401 tttgcacttc tacccatttt ccttttggtc ccattacggt agtatgcaaa ctctcatatc
    17461 catttgcttt tggtgttgaa atccagtcgc gcagacggtc ggggttggga tgatataatt
    17521 cggtaaccat agaatacacg cgccagcaat ctgctttttc gtggtctaca ggtgtttcta
    17581 aaataatacg aatcgcaaat aaatcgtaca cctcatcaaa ggttacgccc ttatttttta
    17641 ttttattgta aatagaataa atactttttg gtcgcccgta catttcaaat ttaaatccgt
    17701 ctttttccaa acgttcacgt aacggcttca taaattcgtt catgtagcgc gtgcgttcgc
    17761 gtttggtttc agcaaggcgt ttggcactat ctttatatgc ttctgtctcc tggtatttca
    17821 taccaagatc ttctaattca cttttgatgg cataaaatcc taatcgatgc gcaagtggtg
    17881 catataaata caatgtttcc gatgcaattt ttaattgttt tttgcgcggc atactatcca
    17941 tggtgcgcat attgtgcaat cggtcggcaa tttttattaa aattactcgc acatcatctg
    18001 cgagtgtaag taataatttt ttgaaatttt ctgcttgtat cgaactcgtt aaatcaaata
    18061 cacctgatac ttttgtcaac ccatcaataa tacgacttac tttaattccg aattcgcgtt
    18121 taatatcatc gagtgttaat tcggtatctt caacaacatc atgtaacaat gcacaaatag
    18181 ctgcggttgc tccaaggccc atttcattaa ccacaatctg agcaacacgc aatggatgga
    18241 tgatatacgg ctccccgctt ttgcgacgca tgtttttgtg ggcgtcgaca gccatttcaa
    18301 atgccaaacg aatttgtttc cgttcggcgc cggtcatatt attccgcaaa cccctcaaca
    18361 agtttttata ttccttgagg atgagtttct tttctttttc gagatctaat ggttccgcgg
    18421 cttccatgat tacgtataaa ttggtgataa acagtctgtt agtctaaact gagaccatac
    18481 tagtttagac tcattgcaat atagacctac aaatctaaaa tataatcaaa aaaaaaggaa
    18541 aatagttttg aacggggcga tgtttgttga ttttggaccc caaccgccta aagttgtcgc
    18601 agacaacgtt gtcgtggagt ctttgactcc agacgccacc ccggt
//
LOCUS       DAORKW010000018        18387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_29_length_18387_cov_5.165939, whole genome shotgun sequence.
ACCESSION   DAORKW010000018 DAORKW010000000
VERSION     DAORKW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18387
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29_length_18387_cov_5.165939"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3957)
                     /locus_tag="PLB46_01655"
     CDS             complement(<1..3957)
                     /locus_tag="PLB46_01655"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF75244.1"
                     /translation="MFLLFSMITQGFNQAVIVGTEDGANMENTYPSPFATYFKSHKAQ
                     YLYTALELQNAGLTAGLINQISWNVLDLPLTPAICSGFSIKLLATETSSLSVTNWESG
                     ATLVWGPSDYTAIPGENIFELTTPFEWNGSANLIVEVCSGTTDALAGGNAIIAWSGPF
                     VNNVSHSYADDVTDAICDYTGETALITETGGSLYRPVMAFGSTPLTCDELWDGYPINV
                     ATTTICPLQEIHINTTAFENPLITYSWYQSADGENWDLILDETNNDIILSPLASTWYR
                     SGAKCPYTDIINFSSPVLITTSTGIECACTPQTNFGTVSGDFISNVALEDINNITEQL
                     ESPYYFKYTESTTSLFTGNNYPLQISVGSRASQNTIAAWMDFNADGVFSAEEKIGEVN
                     NLNAFEVTTLNCFIPITAAIGETILRIRETYNASEIDPCAAYDYGETEDYLITIQSTP
                     EAVADFSYSGQPTVNFTDLSVGLITNWEWDFNDGFIATEQNPVHTFLSNGTYNVCLTA
                     GNVVSSNVICKEIIIDTYIPATANFSFSGDPVVTFTDLSTGDPTTHIWDFGDGVIVEE
                     INPVHTYLMNDTYTVCLTVMNDFSENTFCQDITIENNMPLIAAFNFSGDPEVLFTDLS
                     TGEINSWNWDFNDGYFSEEQNPIHLFANNGIYNVCLTVEGITGSNVMCQEVSIEGHPS
                     PVAHFNYAGDPVVTFTDLSVHIPTTWNWNFGDGNFSSEQNPTHNYLINGVYEVCLTAS
                     NIHGENTYCEFIAINGNTFTPDANFEFIGDPTVTFTDLSANSPNNWYWNFDDGYFSFE
                     QNPTHEFTTNGTYNVCLTASNIAGDNTFCSTINIVNNYETPVAIFEFIGDPTVSFTDI
                     STNNPTAWFWDFDGEGTSTLQNPTFTFLENGEYEVCLTASNPSGFNVNCQTVAITNYA
                     EPIANFTYTNQPLVEFYDASSGVPTSWYWEFDDGTFSLLQNPTHLFLENGTYNVCLTA
                     HNIHGSNTTCQDIIIDSYTAPVADFIFSGDPNVTFTDNSLNVPASWYWIFGDGGISTD
                     QNPIHLFTENDTYNVCLFVTNDIGTDVICQDVVIDGYTISPTAAFSTSGDPIVEFTDE
                     SLNSPSMWYWDFGDGFTSTLQNPVHEYISNGTYNVCLTATNIVGSDTYCEVVVVDTYL
                     PPSAEFTYSGDPVVTFTDLSTYSPNTWFWSFDDGTYATEQNPIHNFVSNGNYDVCLTA
                     TGAGGTNTWCTEITIITNGVAPSVDFNYAIDKNTILFTDNSAGMPTNWFWDFGDGSFS
                     AIQNPVHTYAAATTYYVCLTASNTYGENTVCKTIDFLSMDNSFTSDIQIFPNPSTHF"
     gene            complement(4337..7135)
                     /locus_tag="PLB46_01660"
     CDS             complement(4337..7135)
                     /locus_tag="PLB46_01660"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF75245.1"
                     /translation="MRTLRLLVVTCLSYFALFSTTSAQFTIGDDNGANTATSYPTPIF
                     DYFKTMKSQYLYLASEMTGEGMTAGFITEIRWNVVGVPVGTGTTEDYTIKLLETNTTS
                     LGITSWEPGASLVWGPMDYLPTVGTNTFVLDNPFYWNGTSNLIIEICGGDNVADYTKN
                     ARVTWSGPLGFNASRTYANDAESSPCEYTGSDYYDATPGGFDYRPRVTFATEAAINCN
                     ELPIIGATSSTDVMVCEDELFTLSIGAVAELGIEYAWFSSPNGISWTVIPGADGPSLT
                     TSQSVPTYYRCTVSCSFSGDNSNSIPILIGINDPSDCYCTPAYLLGTTSGDYVSNVTL
                     GDINNSSGPAIVPYYTYFDALSTDLNEGETYTISITCGEYETDNGIAAWIDFNADGVF
                     SASEKLGEGTGLTGFETVTFDFLVPAGSVPGTTRMRIRDVYDIIGIDACAGYEYGETE
                     DYNVNIIEGVPPLAAFSFSGDPTVSFTDLSTGEPTSWLWNFDDGFTSTFQNPTHTFAT
                     NGTYDVCLTATSILGSNTDCNAVTIDSYLPPVADFSYTGDPEVFFTDLSLNDPTSWNW
                     AFGDGFTSPEKNPVHTFAEDGSYYVCLTATNLLGSNTSCEFIEITGHPETPAADFTFS
                     GDPVTAFTDLSANEPTSWYWDFDDGAFSSDQNPTHEFTSNGTYDVCLTASNAAGENTS
                     CETVVITSYAAPDALFTYTGDPSVTFTDLSTNGPVSWLWTFDDGDVSTEQNPVHTYTL
                     NGTYNVCLSVTAPGGTDNYCIDITIAANQSAPVADFSYEISGLIVEFTDLSTNAPESW
                     YWDFDDGIISGLQNPSHTYSERDIYEVCLKATNDAGFTETCKTIDLNTGITLQEIKNI
                     SLFPNPAVDFVTIELPEIMDNLTVKLYNTAGQLVTTDINITSPTSLTINTHLLAAGSY
                     TILVHNSTKFGSISFVKQ"
     gene            complement(7535..10627)
                     /locus_tag="PLB46_01665"
     CDS             complement(7535..10627)
                     /locus_tag="PLB46_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF75246.1"
                     /translation="MKKIFTLIIGICSFAALQAQSTVVGTTTYDLQTNGGNKSRLLVH
                     DDGTVSAMWTGSTSGTISFTDRGMFFNSSPDGSTWGAFPTARIEPTRTGFGDMVKVGD
                     HEVMFSHDGTNIYVFKNDEFGGTTWTPTAGSLQITGLWPNAYCPEGTDDIYVVNANSS
                     TPTGVYFSKSTDGGESWDILEYTLPYLTTDEGFGAIGGEAYQIAVYGSDVYVLYGANY
                     ADLVLLHSDAGGAAGTWTSEIIWDSPLENYTAAAGQTTDYDEDGDYDTILTTDGNYDM
                     LMTDDGTLHVFAGTMYILDNNIAAGYSYFPTIGGILYWKTGLTGMYYLDVVIDWKNDD
                     GLDDPYAGIGPSFAAYGGESFTTLPSSAYDPASGRIYLTYTMPVEYTDQLGDPTAAAA
                     ESKNDLFGIYSDDNGESWTFPVNLTYTAFAGQENYFPMVYDRVVDGKIHVLWQQDDNP
                     GTAVDAAPADAIHTNNLRYAVWDETRFQPYAPNVDFTYTLTPAGPLFNAVFTNLSADA
                     ETYFWDFGDGFTSTLTNPTHTYAEGVYEVCLTGFNVYGEATVCETIIAVFPPTALFDY
                     SGDPTVDFTDLSTNEPTSWAWDFDDGGTSALQNPTHTYALNGIYNVCLTASNVGGAST
                     YCENVVISTYAAPLANFTFAGDPTVTFTDLTIGDPTSWDWTFDDGGVSTEQNPVHTYA
                     TNGTYNVCLTATNALGTSTSCQDVTIGSYLPPVALFTYSGDPLVTFSDLSTESPSLWS
                     WDFGDGDFSTEQNPVHNYTENGTYTVCLTATNDIGSGSSCQTVVIDFYPLPEAIFTFT
                     GDPTVTFTDLSTNSPDSWFWNFDDGGFSSEQNPVHTFTTNDTYNVCLEVTNAGGVDVV
                     CQDVVIGNVVITPVADFDYFFGVDLTVSFFDNSTNSPSNWSWNFGDGETSDLQDPMHV
                     YAAPGSYNVCLTAGNAGGSDEYCQQITLTGIETNTAISLTAYPNPASEIIIIEMPQTQ
                     SDVTVQVINAIGQKIAIEPIMNGNNTSISLNIESLPGGAYTVAINAASIKYIATFIKE
                     "
     gene            complement(10906..12234)
                     /locus_tag="PLB46_01670"
     CDS             complement(10906..12234)
                     /locus_tag="PLB46_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HRF75247.1"
                     /translation="MQFPPGFIHHISQCKGFDEVAFSEAHQLPAPVSIRYNPAKPVEK
                     MAGTPVPWCTDALYLPERPLFVFDPLFHAGTYYVQEASSMFTGYLFQQFTDNKKPLHI
                     LDVCAAPGGKSTLIAGLMSPQSLLVSNEVIKSRVTILKENIVKWGNPNVVVSNNDPRD
                     FASVNGFFDVIIVDAPCSGSGLFRKEPEAKKEWSLDNVNHCSLRQQRILDDIIPALKP
                     GGLLLYATCSYAYEEDEKIVEFLLENDFESLPQINTPAEFGDIVYSQSGYRFYPDKIT
                     GEGFFIAALRKKNGSDEQINQVKKQFISISVDEKNIVSHYLHQPENYFFFKYKEVIYC
                     IPEILKSSISALGGLHLIKSGTALGSIKNNELIPDHELAMSCIISKNIPRLELNATDA
                     ITYLRKNTLQINSNLKGWSLITHQHIPLGWAKIIPGRLNNYFPTEWRIRK"
     gene            12399..16394
                     /locus_tag="PLB46_01675"
     CDS             12399..16394
                     /locus_tag="PLB46_01675"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF75248.1"
                     /translation="MKRHASVFVFLAILCGIVNTQVVINEVTSRGSLIDASGEEIDWI
                     EIYNAGFSTVNLQDYGLSDDALLPLKWQFPNYNLAPSTHMLVLANGVNSYATTNHWET
                     AVVSNDTWKYFVGTSEPPSNWETPAFDDGSWLSGTGGIGFGDGDDGTTITSTISVFTR
                     REFIIDDVDVIEGAKLHADYDDAFVAYLNGVEIARSGNIIGSPPAYNTTANTDHEAGM
                     YAGYLPEEWNIDAAIINSLIVEGNNYLCIQTHNVTAGSSDISSNFWLSFGINTADTYF
                     YAVPAWFVAPYEFNQTNFKLSNTGETLYLSDPTGIIIDSKYTGDIAYGQSIIRKPDGA
                     ASWCITTDITPGYTNNFSSCYAGYEPDPVFSVGSGFYPDATLLYLSVGSPTAVIRYTT
                     DGSAVTESSPLYTGAILLSANTIIAAKCFSTGDLLPSKLIRNSYFIDEDDYTLPIVSI
                     SIDPGSLFDFDTGIYELGCCYDVNYPYFGANFWQPWERYGHIEYFTETGIPQWNKDMS
                     LEIHGGWSRAEAQKGFRVDFNNKYDGELDYPLWGAKPDMGPINNFNLRSGGQHVWTYK
                     FQDAFLAKVMKETHIDYEEWQPCFVFINGEPWGLYEIREKADEHFAESNYGVDNNKVD
                     FFNAWSVLNGSDTGLVNLYGSLMSLDPTSTDYFNKFNQYVDYENYMDYYIGEIYYQNV
                     DFGGYYWGANNMKLWREQNGGKFRFIMYDMDGAMGYFGEVPGTNYINLTRNPSYPNTF
                     SQLFDRTLNNIPLRNYFVNRFADLINTIYLQDNMEEIIYNMRDSIVTEIPHQLGAWGA
                     PTVATMDAYIETTLNYNYTRRNTARTHINSSFGLGGQRTVTLAVAPEGAGYIKLNTII
                     PTDLPWSGTYFDGVPVTMTAIANPGYTFNNWSSNDLIPGGSTDIAITINMEESETFTA
                     NFTGAPETPAIVITEINYNSNNDFDAGDWIEIYNNGDIAFDISEWTIRDASDAYKYSI
                     PYGTVLSVAERLIIAQKPELFANIYPTVSNVVGGFNFEFNNNGDNIRIVDIAGNTITS
                     VLYSDAAAWPKGADGTGRTLELVSFGAVPNNPISWFDGCLLGSPGVAYTPCNDAVLFG
                     EINYNSSAAFDAGDWVEVLNATTGSIDLSGWRFVDEEDSSIFVFEDGLILEANERLVI
                     ANDITKFNAIHPTVTNYIGPFAFGLNSDGDELRLFNADGILQFTMIYNNEAPWPISPD
                     GGSYTLELLDVNGKMNNADNWFAGCQLGSPAEVFNPDCLVDIENLEEADFSIYPNPAS
                     AYFIISTGEYAQLETVVQIIDASGKIIQEQTVIAADKIIVNTTQLWSGIYWVKISNTN
                     LQASKALVIEN"
     gene            complement(16435..17406)
                     /locus_tag="PLB46_01680"
     CDS             complement(16435..17406)
                     /locus_tag="PLB46_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRF75249.1"
                     /translation="MSVLGKIQQPIASELALFESKFKESMKSNVALLDRVMYYIVKRK
                     GKQMRPMFVMLSANVCGGISESTYTAASLIELLHTATLVHDDVVDDAYERRGFFSINA
                     LWKNKIAVLIGDYLLAKGLLVSLDNNEHRLLKITSTAVKEMSEGELLQIEKARRLDIK
                     ESIYYEIIRKKTASLIASACSAGAASATKDESLIETFRLFGEKIGIAFQIKDDLLDYG
                     DEDIGKPLGIDIKERKLTLPIIYTLQNASKADKQKIINIVKNENKNKAKVDWVITFVK
                     QNGGIQYAEQKMRAYQAEAFELLNQFPDSESKNSLIELVKYTIERRK"
     gene            17498..18295
                     /locus_tag="PLB46_01685"
     CDS             17498..18295
                     /locus_tag="PLB46_01685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRF75250.1"
                     /translation="MMKLKTPVIIAAFAAVLLTNSCSEGGGINIFSLEDDIALGAQIA
                     AEIESDPATYPILDSADYATAYGLIYDIRDRILASGNVYHKDDFEWQIRIINNDEVVN
                     AFCTPGGYIYVYTGLIKYLEDESQLAGVLGHEMAHADLRHSTDQLTEAYGISLLLSIV
                     LGEQESVLADVAASLTQLAFSRGDESQADEYSVIYLCPTDYVASGSAGFFVKVEEEGG
                     VEIPEFLSTHPNPDNRIENINAAAEELACEGEDIFTDRYDDLLDALP"
BASE COUNT         5788 a         3269 c         3610 g         5720 t
ORIGIN      
        1 aaaatgagtg ctaggatttg ggaatatttg aatatcagat gtaaatgaat tatccatgga
       61 taaaaaatca atggttttac aaaccgtatt ttcaccataa gtatttgaag cagtcagaca
      121 tacataatag gtagttgcag cagcataagt atgtaccgga ttttgaatgg cagaaaatga
      181 accatcacca aaatcccaaa accaattagt tggcatacct gccgaattat cggtaaataa
      241 tatggtgttt ttatcgatag cataattaaa atcaacacta ggagcaactc cattagtaat
      301 aatggtaatt tcagtgcacc aagtattagt tccacctgca cctgttgctg ttaaacatac
      361 atcataatta ccattgctga caaagttgtg aattggattt tgttcggtag cataagtgcc
      421 atcatcaaaa ctccaaaacc atgtatttgg agaataggtt gacaaatcgg taaacgtaac
      481 tacaggatca ccggagtagg taaattctgc tgaaggtggt aaataggtgt caactaccac
      541 aacttcacaa taggtatctg agcctacaat attagttgca gttaaacaga cattgtaagt
      601 gccatttgaa atatattcat gaacaggatt ttgtaaagtt gatgtaaatc catctccaaa
      661 atcccaatac cacatggaag gagaattcaa actttcatct gtaaattcaa caataggatc
      721 accacttgtg ctaaatgcag cggttggact aattgtataa ccatcaatta ctacatcctg
      781 gcaaattaca tctgttccaa tatcgttggt tacaaataaa caaacattat aagtatcatt
      841 ttcagtaaag aggtgaatag gattttgatc agtagaaatt ccaccatcac caaaaatcca
      901 ataccaggaa gcaggcacat ttaatgagtt gtctgtaaag gtaacatttg gatcaccgct
      961 aaaaataaaa tctgcaactg gtgcggtata actatctatt ataatatcct gacatgttgt
     1021 attagaccca tgaatattat gagctgttaa acaaacatta taagtaccgt tttctaaaaa
     1081 taaatgtgta ggattttgta acaatgaaaa tgttccgtca tcaaattccc aataccaaga
     1141 ggttggcact cctgaacttg cgtcataaaa ttcaactaac ggttggttcg tataagtgaa
     1201 attcgcaatg ggttcggcat aattagtaat ggcaactgtc tggcaattta cattaaagcc
     1261 ggatggattt gatgctgtta aacaaacttc atattcaccg ttttctaaaa aggtgaatgt
     1321 tggattttgt aatgttgagg tgccttcacc atcaaaatcc caaaaccatg ctgttggatt
     1381 attggtgcta atatcagtaa atgaaacagt tgggtcgccg ataaattcaa atattgcaac
     1441 cggagtttcg taattattta caatattaat agttgaacaa aaagtattgt caccagcaat
     1501 atttgatgct gttaaacaaa cattgtaagt gccgtttgtt gtaaactcat gtgttggatt
     1561 ttgttcaaat gaaaaataac catcgtcaaa attccaatac caattgttag gactatttgc
     1621 acttaaatca gtaaacgtaa cggtcgggtc tccgataaac tcaaaatttg cgtccggtgt
     1681 aaaagtatta ccatttatgg ctataaattc acaatacgta ttttcaccat gtatatttga
     1741 agccgttaaa catacttcgt atacgccatt aattaaataa ttatgtgtag gattttgttc
     1801 ggaagaaaaa tttccatcgc caaaattcca gttccatgtt gtagggatgt gcacagataa
     1861 atcagtaaac gtcacaactg ggtcgccagc ataattaaag tgagcaacag ggcttggatg
     1921 accttcgata ctcacttcct gacacatgac gttactaccg gttatgcctt ccacggttaa
     1981 gcaaacatta tagatgccat tatttgcgaa tagatgaata ggattttgtt cctcagaaaa
     2041 atagccatca ttaaaatccc aattccaact atttatttca cctgtagata aatcagtaaa
     2101 caatacttca ggatcgccac taaaattaaa cgcagcaatt aatggcatat tattttcaat
     2161 ggtgatgtcc tgacaaaaag tattttcaga aaaatcattc ataacggtga ggcatactgt
     2221 ataggtgtca ttcattaaat acgtatgcac cggattaatt tcttcaacaa taacaccatc
     2281 gccaaaatcc caaatatgtg tagttgggtc accggtagat aaatcggtaa atgtaactac
     2341 cgggtcgccg gaaaaactaa aattagctgt tgccggaata taagtatcaa ttataatttc
     2401 cttgcaaata acattactgc tcacaacatt acctgcagtt aaacaaacat tgtaggtgcc
     2461 gtttgataaa aaggtatgca caggattttg ttcggtagca ataaaaccat cattgaaatc
     2521 ccattcccaa ttcgtaatta aacctacaga taaatcggta aaatttacgg ttggttggcc
     2581 gctatatgaa aaatcggcaa cagcttcagg tgtactttga atggtaatta agtaatcttc
     2641 tgtttctcca taatcatatg cagcacaagg gtcaatttct gatgcattat atgtttctcg
     2701 tattcttaat attgtttccc caattgctgc agttattggt ataaaacaat ttaatgttgt
     2761 aacttcaaaa gcgtttaaat tatttacttc accaattttt tcttccgcgc taaaaacacc
     2821 atctgcatta aaatccatcc acgctgcaat tgtattttga gatgctctgc tgccaacact
     2881 aatttgtaat gggtaattat ttccggtaaa taatgaggta gtgctttcgg tatatttaaa
     2941 atagtagggg ctctctaatt gttcggtaat attattaatg tcttcgaggg caacattact
     3001 aataaaatca cctgaaacgg tgccgaagtt tgtttgcggt gtacaggcgc attcgatacc
     3061 ggttgaagtt gttatgagta ccggagaaga aaaattaata atgtcagtat acggacattt
     3121 agcaccactg cgataccatg ttgaggctaa tggcgataat atgatatcgt tattagtttc
     3181 atctaatatt aaatcccaat tttcgccatc cgcagattga taccaactat aggtaattaa
     3241 tggattttcg aatgctgtgg tattgatatg tatttcctgt aaaggacaaa ttgttgtagt
     3301 tgcaacattt atcggataac catcccataa ttcatcgcag gttaaaggtg tgctgccaaa
     3361 agccattaca ggtcggtata aacttccgcc ggtttcggtt attaatgcag tttcaccggt
     3421 gtaatcgcat atggcatcag ttacatcatc agcatatgaa tgagaaacat tatttacaaa
     3481 tggacctgac cacgcaatga tggcatttcc acctgcaagg gcatcggtag ttccgctgca
     3541 aacttcaaca attaaattag cgctaccatt ccattcgaaa ggagttgtta attcgaatat
     3601 gttttcgccc ggaattgctg tataatcaga tggtccccat acaagcgttg cacctgattc
     3661 ccagtttgtt accgaaaggg aagatgtttc agttgcaagt aacttgatgg aaaagcccga
     3721 acaaatagcc ggggttaaag gtaaatccaa aacattccag gatatttgat taataagtcc
     3781 ggctgttaaa ccggcattct gcagctctag agcagtatat agatattggg ccttatgcga
     3841 tttaaagtat gtggcaaacg gtgatgggta agtgttttcc atgtttgcac catcttcagt
     3901 tccgacaata acagcctggt taaaaccttg tgttatcatc gaaaaaagaa gaaacataaa
     3961 cagatttgtg ggtaaaaatc ttttcatgtt gtgtgactag ttaaatcttg ttttcgattt
     4021 agtttttggg aaataattat tcggtaaata taaaacacct ttcctcaaaa ccctttattt
     4081 tgtggaaaat tcacggttta cggtgaataa gaagattaaa ttctaccatt atttgaccca
     4141 aaaaaagtac aaaaaaacaa taatatgtga catctaattt accggtatgg gctgagtcgt
     4201 aaaatttgga aaaaatatac tttatttgtg acaaggtacg tttgccgata tatttgtaag
     4261 gattaattcg aaatggtcga tgcataaaaa aaccaccgaa tttggtggtt tttttttgta
     4321 taaaaagaga acttagttat tgcttgacaa aagaaattga gccaaatttg gtggaattgt
     4381 gaactaaaat tgtatatgac ccagcagcaa gtaaatgcgt gttgattgtt aaacttgttg
     4441 gactggttat attgatatca gtcgtcacaa gttgaccggc ggtattatat agttttacgg
     4501 ttaaattgtc cataatttcc ggaagttcta tagtaacaaa atctacagca ggatttggaa
     4561 ataagctgat gtttttaatt tcctgtaatg taattccggt atttaaatcg atggttttac
     4621 aagtttctgt aaagccggca tcgttggttg ctttgaggca aacttcataa atgtctcttt
     4681 cactataagt atgtgaagga ttttgtagac cggagataat accatcatca aaatcccagt
     4741 accagctttc aggagcgttg gtagacaaat cggtaaattc aacaattaat ccggaaattt
     4801 catagctgaa atcggcaacc ggagctgatt ggtttgcagc gatagtaata tctatacaat
     4861 aattatcggt tccgccaggt gcagttacag ataaacatac attataagtg ccgtttaagg
     4921 tataggtatg taccggattt tgttcagtgc ttacatcacc atcatcaaaa gtccaaagcc
     4981 aggatacagg gccgttagtt gaaaggtctg taaatgttac agaaggatcg cctgtgtagg
     5041 taaataatgc atccggtgct gcataagaag taattacaac tgtttcgcaa gatgtatttt
     5101 caccggctgc atttgaagct gttaaacaaa catcgtaagt gccgttagaa gtaaattcat
     5161 gagtcggatt ttggtcggaa ctaaatgcgc catcatcaaa gtcccaatac caagaagttg
     5221 gttcattggc actgaggtcg gtaaatgcgg taacgggatc accactgaaa gtgaaatcag
     5281 ctgcaggtgt ttccggatgg ccggtaattt ctatgaattc acaagatgtg ttagaaccta
     5341 ataggtttgt agctgtgaga caaacataat aggagccgtc ctccgcaaaa gtatgtacag
     5401 ggtttttttc cggagaagta aacccatcac caaaagccca gttccaagag gtagggtcgt
     5461 tcagcgataa atcagtaaaa aagacttcag ggtcgccggt ataactgaaa tctgcaaccg
     5521 gtggcaaata ggaatcaatt gtaacggcat tacaatcggt attactacct aaaatactgg
     5581 ttgctgttaa acaaacatca tatgtgccat ttgttgcaaa agtatgagtt gggttttgaa
     5641 aggtggaagt aaatccatcg tcaaaattcc acaaccaaga agttggttcg ccggtagata
     5701 ggtcggtaaa tgatactgtt gggtcaccgc taaaactaaa tgcagccagc ggtggaacac
     5761 cttcgataat atttacattg taatcttcag tttctccata ttcataacct gcgcaggcat
     5821 ctatgccaat aatatcgtat acatccctga ttcgcattct tgtagtaccg ggaacacttc
     5881 ctgcaggaac aaggaaatcg aatgtaacag tttcgaaacc ggttaagcct gttccttctc
     5941 ctaatttttc ggaggcacta aatacaccgt cagcgttaaa atcaatccag gcagcaatac
     6001 cgttatcagt ttcatattcg ccacatgtaa ttgaaatggt ataagtttca ccttcattta
     6061 aatcggtaga taatgcatca aaataagtat agtaaggcac tatagcagga cctgaggagt
     6121 tattaatatc accaagtgtt acattactta catagtcgcc ggatgttgta cctaataagt
     6181 aggcaggagt gcaatagcaa tcgctagggt catttatacc gattaaaata ggaattgaat
     6241 tagaattgtc gccactaaat gagcaactaa ctgtgcaacg ataatatgtt ggtacactct
     6301 gcgaagtagt gagagaaggt ccgtctgcac ctggaataac tgtccatgaa atgccgtttg
     6361 gcgatgaaaa ccaagcgtat tcgataccta attcagcaac agcaccaatt gaaagcgtaa
     6421 aaagttcatc ttcacaaacc attacatcgg tactggaagt agcaccaatt atcggtaatt
     6481 cgttacagtt aatagcagct tcagttgcaa aagttactct cgggcgatag tcgaaaccac
     6541 cgggagtcgc gtcataataa tcgctgcctg tgtattcaca aggactgctt tcagcgtcgt
     6601 ttgcatatgt tcgagaagcg ttaaatccaa gtggacctga ccatgtaacg cgtgcgtttt
     6661 tggtatagtc ggctacatta tcgcccccgc aaatttctat aatcaaatta gaagtgccgt
     6721 tccagtaaaa aggattgtcc agtacaaacg tattggtacc aactgtcggc aaatagtcca
     6781 taggtcccca aactaaagat gcaccgggct cccaacttgt tatccctaac gaagtggtat
     6841 tggtttccag taattttatt gtataatctt cagtagttcc ggttccaacg ggcaccccta
     6901 caacattcca tctgatttct gtaatgaatc ctgcagtcat tccttcgccg gtcatttcag
     6961 aagccaaata caaatattga gacttcattg ttttaaaata atcaaaaatt ggtgtaggat
     7021 aacttgttgc tgtattggcg ccattatcat caccaatagt aaactgtgcc gatgtggttg
     7081 aaaaaagcgc aaagtaagat aaacaagtta caaccaggag tcgtaatgtc ctcattttgt
     7141 gtgtgttaaa ttttaaattt tagtaaaact gtgttttcta gctttcaaat gtagcgattt
     7201 ttaaatttct tataaaacaa attgcaattt aattgtcata tgaaattgca taaattttat
     7261 tagaaccatc caccctgcct gattcctgct ttttttcttc aaattttaaa gtcggtttat
     7321 ttcgtcacat ttttttattt tttgtaagtt tttttgcctt ttagttcaaa atgtaaataa
     7381 aacactggaa aaagcctagg gctattttaa gctgaatttt ccattaattg catttaacat
     7441 ttttcagcgt tgctgctaaa tagcattact aaaagtgcaa aaaaaagcag ctgttaattt
     7501 aataacagct gctttttaca atattaaaag gtatttattc cttaataaat gtagctatat
     7561 atttaattga agctgcattt attgcaacag tataagcacc tccaggtaat gattcaatat
     7621 ttaaagaaat gcttgtatta ttaccattca taattggctc aattgcaatt ttttgtccaa
     7681 ttgcattaat aacctgaaca gtaacatcac tttgtgtttg aggcatttcg ataatgatta
     7741 tttccgatgc aggattagga taagcagtaa ggctaatagc ggtatttgtt tcaatacctg
     7801 ttaaggtaat ttgctgacaa tattcatcgc taccacctgc atttccagcg gttaaacata
     7861 cattataact tcccggcgct gcataaacat gcatagggtc ttgtaggtca gatgtttcgc
     7921 catcaccaaa attccatgac cagtttgaag gtgaattggt tgaattatca aagaaactca
     7981 ctgtgaggtc tacaccaaag aaataatcaa aatcagcaac cggtgttatt acaacattac
     8041 caataactac atcttgacaa actacatcaa caccacctgc attggtaact tcaagacaaa
     8101 cattataggt atcgttagtg gtgaaggtat gtaccggatt ttgttctgag ctgaaaccac
     8161 cgtcatcaaa attccagaac cagctatcag ggctgttggt agataaatcg gtgaaagtta
     8221 cggttgggtc tcctgtaaat gtgaaaattg cttcgggtaa cggataaaag tcgataacaa
     8281 ctgtttggca gctgctacct gagccaatat cgttagtagc agttaaacaa accgtatagg
     8341 tgccattttc cgtgtagtta tgaaccgggt tttgttcggt gctgaaatct ccatcaccaa
     8401 aatcccatga ccataaacta ggtgattcgg tagagaggtc ggaaaatgtt accagcgggt
     8461 cacctgaata agtaaataat gcaaccggtg gtaaatagct gccaatagtt acatcttgac
     8521 aacttgtgct ggttccaagg gcatttgttg ctgttaaaca aacgttatat gtgccgtttg
     8581 ttgcataagt atgcaccgga ttttgttcag ttgaaacccc gccatcatca aatgtccaat
     8641 cccatgaagt tgggtcacca atagttaaat cggtaaaagt aacagtaggg tcgccggcaa
     8701 aagtgaaatt ggctaacggt gcagcgtagg ttgaaataac cacattttcg caataggtag
     8761 atgcgccacc aacattagat gcggtaaggc acacattata aataccgttt aaagcataag
     8821 tatgtgtagg gttttgaagc gcacttgtac caccatcgtc aaaatcccat gcccagcttg
     8881 taggttcgtt ggtagaaagg tctgtgaaat cgacagtagg gtcgccactg taatcaaaaa
     8941 gtgctgttgg cgggaatacc gcaattatag tttcacaaac tgttgcttca ccatacacgt
     9001 taaagccggt taaacacact tcataaacgc cttcagcata ggtatgtgta gggttggtta
     9061 atgttgaagt aaaaccatca ccgaaatccc agaaataagt ttctgcatca gcgcttaaat
     9121 tggtaaatac agcgttaaac aacggacctg ctggtgttag cgtgtatgta aaatctacat
     9181 ttggagcata aggttgaaaa cgtgtttcat cccaaacggc ataacgtaaa ttattggtat
     9241 gaatcgcatc ggcaggagca gcatcaacag cagttcccgg attatcatct tgctgccata
     9301 acacgtgaat ttttccatca acaacacggt cgtaaaccat tggaaaataa ttttcctgac
     9361 cggcaaacgc ggtgtaggta aggtttacag ggaatgtcca tgattcacca ttatcatctg
     9421 aataaatacc gaataaatcg tttttcgatt cagcagcggc tgccgtaggg tcgcctaatt
     9481 ggtcggtata ttctaccggc atagtatagg ttaaataaat tcttcctgaa gcaggatcat
     9541 atgcacttga aggtaaagtg gtaaaacttt caccgccata agcagcaaaa gaaggaccga
     9601 taccagcata tggatcatcc aaaccgtcgt cgtttttcca gtcaattact acatcgagat
     9661 aatacatacc ggttaatcct gttttccagt ataaaatacc gccgatagtt gggaaatagc
     9721 tataaccggc agcaatattg ttatctaaaa tatacatagt gccggcaaat acatgaaggg
     9781 taccgtcatc agtcattaac atatcataat taccatcggt agtcaggatt gtatcgtaat
     9841 cgccatcttc gtcataatcg gtagtttgtc ctgcagccgc ggtataattt tcgagtggac
     9901 tatcccaaat aatttcagaa gtccaggtac ctgctgctcc gccggcatca gaatgcaaca
     9961 atactaggtc agcatagttg gcaccatata atacatacac atctgaacca tatactgcaa
    10021 tttgataagc ttcaccgcca atagcgccaa aaccttcatc agttgttaaa tacggcaggg
    10081 tatattctaa aatatcccag ctttcaccac catcggttga ttttgaaaaa taaactccgg
    10141 taggtgtaga ggaattggca tttaccacat aaatgtcgtc agtaccttca gggcaataag
    10201 catttggcca taaaccggtg atttgtaaag aacctgcagt aggtgtccaa gttgtgccac
    10261 caaattcatc atttttaaaa acataaatat tggtaccatc gtgtgaaaac attacttcat
    10321 ggtcaccaac ttttaccata tcaccaaaac cagttctggt gggttcaatt cttgcggttg
    10381 gaaatgcccc ccaggtggat ccatcgggtg aagaattaaa aaacatgccg cggtcagtaa
    10441 atgaaattgt tccgctggta gaaccggtcc acatagcaga aacggttccg tcgtcgtgta
    10501 cgagtaaccg acttttattg cctccattcg tttggaggtc gtaggtggtt gtgcctacaa
    10561 cggtagattg tgcctgcaaa gctgcaaagg agcaaatacc gataatgagt gtaaagattt
    10621 ttttcatagt aaaaattata tctaaagagg gaggaaaatt ataaaaaaaa ttgaagaatt
    10681 taattttcat gataaggata caatcacaaa taccactata tccatgaaaa tatttgcctt
    10741 gttatttgcc ggtttaaatg tgtttatgtt catgatcaga aacagattca atgcactaac
    10801 gagacgtttc gttttttgta atttacctaa tgcctgagtc tcccggtgtt ggagtgtatt
    10861 ttttctatta aaatttgaac taccgaaata aaaagggcaa aacaattatt ttctaatcct
    10921 ccattcagtt ggaaaataat tgtttaatct gccgggaatt attttcgccc aacctaatgg
    10981 aatatgttga tgcgtaatta aactccagcc ttttaagtta ctgttaattt gtaaggtatt
    11041 ttttcgcaaa taagtgattg catctgttgc atttaattcc aaccgcggaa tgtttttcga
    11101 aataatacaa ctcatagcta attcatggtc aggaattaat tcgttgtttt tgatacttcc
    11161 taatgccgtg cctgatttaa taagatgtaa tccacctaat gcactaatgg atgattttaa
    11221 tatttcggga atacaataaa ttacttcttt atacttaaaa aagaaataat tttccggttg
    11281 atgcaggtaa tgtgagacta tatttttttc gtcgacacta attgagatga attgtttttt
    11341 gacctgatta atttgttcat cgctcccatt ttttttgcgt aaagcggcta taaaaaatcc
    11401 ttcaccggta attttatcgg gataaaaacg atatcctgac tgactgtaca caatatcccc
    11461 aaactcagca ggggtgttta tttggggcaa agattcaaaa tcattttcta ataaaaattc
    11521 aacaattttt tcatcttctt cgtaggcata tgagcaggtt gcatatagta ataatccacc
    11581 cggctttaat gcaggaatga tgtcatctaa aatacgttgt tggcgcaagc tgcaatgatt
    11641 gacattatcg aggctccatt cttttttcgc ttcaggttct ttgcgaaata aacctgaacc
    11701 actacaaggt gcatctacaa taataacgtc aaaaaaacca ttaacgctgg caaaatctct
    11761 agggtcgtta ttacttacta ccacatttgg gttgccccat tttacaatat tttctttgag
    11821 aattgttacg cgcgatttaa taacttcatt acttaccaat aaagattgtg gagacattaa
    11881 tccggcaatt aaagtagatt ttcctccggg tgcagcacat acatctaaaa tatgtaatgg
    11941 ttttttatta tcggtaaatt gctgaaacaa ataacctgta aacatactgg aggcttcctg
    12001 cacataatag gtgcctgcat gaaacaacgg gtcgaagaca aataatgggc gttcgggtaa
    12061 atacaatgcg tcggtgcacc aaggaaccgg ggtgccggcc attttttcga ccggtttagc
    12121 aggattgtaa cgaattgaca ccggtgcagg caattgatga gcttcagaaa atgcgacttc
    12181 atcgaaacct ttacattggc tgatatgatg gataaaaccc ggcggaaact gcataaaacc
    12241 tttgttgcaa aagtaaggtt aaagtgcagg atttgagtaa atcgaatttc ttttttgttg
    12301 aattttgcct tctacaacaa ttaaacttgg tggattaaag ccgttttcaa ttgcagctca
    12361 caaagaaata gttaacttta tattcatatt cagttaatat gaaaagacac gcatcggttt
    12421 ttgttttcct ggcaatttta tgtgggatag ttaatactca agttgttata aatgaagtta
    12481 cttcccgtgg ttcactgatt gatgcaagtg gtgaagaaat tgattggatt gaaatttata
    12541 atgccggatt ttcaacagta aatcttcaag actatggatt aagtgatgac gcattattgc
    12601 ctttgaagtg gcaattcccg aattacaatt tagcgccatc aacacatatg cttgttttag
    12661 ctaacggggt taacagttat gctactacta accattggga aactgcggtg gtgagtaacg
    12721 atacctggaa atattttgtt ggaacaagcg aacctccatc aaattgggaa acaccggcat
    12781 ttgatgatgg tagttggtta agcggcaccg gtggtatagg attcggtgat ggtgatgatg
    12841 gtactactat tacttcaacc atcagtgtat ttacaaggcg cgaatttatt atagacgatg
    12901 ttgatgtaat tgaaggcgca aaattacatg ccgattatga tgatgcattt gtagcatatt
    12961 taaatggtgt tgaaattgcc cgttcaggaa atattattgg ctcaccaccg gcatataata
    13021 ctactgcgaa taccgaccac gaagcaggaa tgtatgcagg gtatttaccg gaagaatgga
    13081 atatcgatgc agccataatt aatagtttaa ttgttgaagg caataattat ttatgtattc
    13141 aaacacataa tgttacggct ggttcttcgg atatatcaag taatttttgg ttatcgtttg
    13201 gcattaatac tgcagataca tatttctatg ccgttcctgc atggtttgtt gcgccatatg
    13261 aatttaatca aactaatttt aaattaagta ataccggtga aacattatat ttatcagacc
    13321 cgacaggtat tattattgat tcaaaatata ccggtgatat tgcttatggc caaagtataa
    13381 ttcgcaaacc agatggcgca gcaagttggt gtattacaac tgatattacg ccgggatata
    13441 caaataattt ttcaagttgt tatgccggtt atgaacctga tccggtattt tctgtcggtt
    13501 caggatttta tccggatgca actttattat atttaagtgt tggttctcca actgcagtta
    13561 tcagatacac aactgatggt tcagcagtta cagaatcatc acctttatat acaggggcta
    13621 ttttattaag tgctaacacg attattgcag caaagtgttt tagcaccggc gatttattgc
    13681 caagtaaatt aataagaaat agctatttta ttgatgaaga tgattacact ttgccaatag
    13741 tttctatttc aattgacccg ggtagtttgt ttgattttga taccggtatt tatgaattgg
    13801 gttgttgtta cgacgtgaat tatccatact ttggtgcaaa tttctggcaa ccttgggaac
    13861 ggtatggaca tattgaatat tttaccgaaa caggtattcc acaatggaat aaagatatgt
    13921 cgctcgaaat ccatggcggc tggagcagag ccgaagcaca aaaaggtttt agggtcgatt
    13981 ttaataataa atatgatggt gaattagatt atcctttatg gggtgcaaaa ccagatatgg
    14041 ggcctattaa caattttaat ttacgaagtg gtggtcaaca tgtttggact tataaattcc
    14101 aggatgcgtt tttagcaaaa gtgatgaaag aaacgcatat cgactatgaa gaatggcaac
    14161 cttgttttgt gtttataaat ggtgaaccat ggggcttata tgaaattcgg gaaaaagcag
    14221 atgaacattt tgctgaaagt aattacggtg tagataataa taaagttgat ttttttaatg
    14281 catggtcggt actcaatgga agtgatactg gattggtgaa tttatatggc agtttaatgt
    14341 cacttgatcc cacatcaact gattacttta ataaattcaa tcaatatgta gattacgaaa
    14401 attacatgga ctattacatt ggtgaaattt attatcagaa tgtcgatttc gggggctatt
    14461 attggggtgc caacaatatg aaattatggc gtgaacaaaa tggtggtaag ttccgattta
    14521 tcatgtatga tatggatggt gcaatgggtt attttggaga agtgccaggt acaaattata
    14581 ttaatttgac acgcaatcct agttatccaa atacattttc acaattgttt gaccgtacat
    14641 taaataatat tccattgcgc aattattttg tcaatcgatt tgcagattta ataaatacaa
    14701 tttacctcca ggataatatg gaggaaatta tttataacat gcgcgactct attgtaacag
    14761 aaattccaca ccagctcggt gcttggggtg caccaacagt tgctaccatg gacgcatata
    14821 ttgaaacaac attaaactat aattatacac gaagaaatac tgccagaacg catattaata
    14881 gttcatttgg attgggcggc cagcgcacag ttactttagc tgtcgcgcct gaaggtgcgg
    14941 gatatattaa actcaataca attatcccaa ccgatttgcc ttggagtgga acgtactttg
    15001 atggtgttcc ggtaaccatg actgctattg caaacccagg ttatacattt aataattgga
    15061 gtagcaatga tttaattccc ggtggttcta cagatattgc tattacgatt aatatggaag
    15121 aaagtgaaac atttactgcc aactttaccg gtgctccgga aactcctgca attgttatta
    15181 cagaaataaa ttataacagc aataatgatt ttgatgcagg tgattggata gaaatttata
    15241 ataacggtga tattgcgttt gatatcagtg aatggacaat tcgcgatgcc agtgatgctt
    15301 ataagtatag tattccttat ggtactgttt taagtgttgc cgaaagatta ataattgcgc
    15361 aaaaacctga gttgtttgca aatatttatc ctacagttag taatgttgtt ggtggattta
    15421 attttgagtt taataataac ggggataata tccgaattgt agatattgcc ggaaatacaa
    15481 ttacgagcgt tttgtatagt gatgcagcgg catggccaaa aggtgctgat ggcacaggaa
    15541 gaacgctgga attagtatcg tttggcgcag tgccgaataa tccaatcagt tggttcgatg
    15601 gttgtttgct gggttcaccg ggtgttgctt atacaccttg taatgatgca gttttatttg
    15661 gtgaaataaa ttataatagt tcggcagcat ttgatgcggg tgattgggtt gaagtattaa
    15721 acgctacaac cggcagtatt gatctttcag gatggcgttt tgttgatgaa gaagattcat
    15781 caatttttgt atttgaagat ggcttaatat tggaggcaaa tgagcgcctg gttattgcca
    15841 atgacataac taaatttaat gctattcatc caactgttac caattatatt ggaccatttg
    15901 cctttgggtt aaatagtgat ggtgatgaat tacgattgtt taatgcagat ggtattttgc
    15961 aatttacaat gatttacaac aatgaagctc catggccgat ttctccggat ggaggcagtt
    16021 ataccttgga attattagat gttaatggca aaatgaacaa tgcagacaat tggtttgcag
    16081 gttgtcagct tggatcaccg gccgaagtgt ttaatcctga ttgtttagtt gatattgaaa
    16141 atctggaaga agctgatttt tctatttatc ctaaccctgc ttctgcttat ttcattattt
    16201 ccacaggtga atatgcacaa ttggaaactg tagtgcaaat aattgatgca tcaggtaaaa
    16261 taatacagga acaaactgtt attgcagctg ataaaattat tgttaacacc acacagctgt
    16321 ggtcaggtat ttattgggtc aaaatcagca acacaaattt gcaagcaagc aaagcattgg
    16381 taattgaaaa ctgatagtga gattgagttg ctgaatagaa aacaatagca gtaattattt
    16441 gcgtcgttca atggtgtatt tcaccaattc tattaaagag tttttacttt cactatcggg
    16501 gaactgattt aataattcaa aagcctcagc ttggtaagca cgcatttttt gctcggcata
    16561 ctgaatgcct ccattttgtt ttacaaatgt tatcacccag tcaaccttag ctttattttt
    16621 attttcgttt tttacgatat taataatttt ttgtttgtcg gctttggacg cattttgtaa
    16681 ggtataaata ataggtaaag taagtttgcg ctcttttata tcgatgccta atggtttgcc
    16741 tatatcttca tcaccataat ccagtaaatc gtctttaatt tgaaaagcga ttccaatttt
    16801 ttctccaaat agtcggaatg tttcaattaa actttcgtct ttggttgcgc tggcagcacc
    16861 tgcactgcaa gcactggcta ttaatgaagc cgtttttttt ctgattattt cgtaataaat
    16921 cgattcttta atatccagtc gacgcgcttt ttcaatttgt aataattcac cttcactcat
    16981 ttctttcact gcggtagagg taattttgag taaacggtgt tcattgttgt ctaacgaaac
    17041 caataaacct ttagctaaaa gataatcgcc aattaaaacg gcaattttat tcttccacaa
    17101 cgcatttatt gaaaagaaac cacggcgttc ataggcatca tcaaccacat catcgtgcac
    17161 taaagtagca gtatgcaaca attcgataag ggaagctgcc gtataggttg attctgaaat
    17221 gccgccacaa acattagcgc ttaacatgac aaacatcggg cgcatttgtt tgcccttgcg
    17281 tttaacaatg taatacatta cacggtcaag caaggctaca ttactcttca tcgactcttt
    17341 gaatttgctt tcaaaaagtg cgagttctga tgcaataggc tgctggattt tccctaaaac
    17401 tgacatgcgg atgcaagtta aggcacattt taattataac aaattaactt tggcttctgt
    17461 tttgctataa acaactcaaa cctaattgcg ttatacaatg atgaaactta aaacaccggt
    17521 tataattgcc gcatttgctg cggttttatt gactaattcc tgttctgagg gaggcggtat
    17581 taatatattt tcactggagg atgatattgc gctcggcgca caaatagctg ctgaaattga
    17641 atcagaccct gctacatatc ccattttaga tagtgcagat tatgccactg cctatggtct
    17701 tatatacgat atcagagaca gaattctggc ctcaggtaat gtttaccata aagatgattt
    17761 tgaatggcag attcgtatta taaataacga tgaagtggta aatgcgtttt gcactcccgg
    17821 cggttatatc tatgtgtaca caggtttaat caaatatctg gaagatgaat cgcagcttgc
    17881 aggtgtacta gggcatgaaa tggcgcatgc tgatttacgg cactctaccg accagcttac
    17941 cgaagcgtat ggtatttcat tactactcag tattgtttta ggtgaacagg aaagcgtttt
    18001 ggctgatgtt gctgccagtt tgacacagtt ggcgtttagc cgcggtgatg aatcacaggc
    18061 cgatgaatat tcggttattt atttgtgtcc taccgattat gtagcctcgg gttctgcagg
    18121 cttttttgtt aaagtggaag aagaaggtgg ggttgaaatt cctgaatttt taagcacaca
    18181 cccaaatccg gataaccgta ttgaaaatat caatgctgca gcagaagagc ttgcttgcga
    18241 gggggaagat atttttaccg accgatacga cgatttattg gatgcactac cataaaaaat
    18301 aatccgcact cgggcggatt attaatcaaa aacaaaataa aaaaaattga aggagatgat
    18361 gataatcaca tctcctgttt taattat
//
LOCUS       DAORKW010000019        18250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_30_length_18250_cov_5.839681, whole genome shotgun sequence.
ACCESSION   DAORKW010000019 DAORKW010000000
VERSION     DAORKW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18250
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30_length_18250_cov_5.839681"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..845)
                     /locus_tag="PLB46_01690"
     CDS             complement(132..845)
                     /locus_tag="PLB46_01690"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRF75251.1"
                     /translation="MFNKFAIILIIFSMGACNKPKNKPAAAVSYPVNGLQLIVVQTHD
                     WSTTSGTMQLLERADTASDWRVVDTFDVMVGRNGMARDKNSSWQQTTNDPIKQEGDGC
                     APSGIFSLGPVFSNHALDNLNMPFKQVNKSDLCVDDINSKYYNTLIDVDTILEKDFNS
                     FEYMRRDDHQYEYGVWVHYNTNPQVPGNGSCIFLHIYKTPNTPTSGCTAMSKENMLKL
                     IHWLNASKNPMLIQYPIHR"
     gene            972..1442
                     /locus_tag="PLB46_01695"
     CDS             972..1442
                     /locus_tag="PLB46_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75252.1"
                     /translation="MKKFLSFVLACTLATFTFSQSPIVLEESDFATGGDSYYIVNANP
                     AITFDAEATGPDYNWDFTDLEVMTATVTNFEDPTDTDPLYFFLWLSSDVAQQNPTDLA
                     NDFITIEDVFNFYKLNNDEFAQTGFAGTISDIPLPIAYDNNEILYQFPSAYSED"
     gene            1563..2042
                     /locus_tag="PLB46_01700"
     CDS             1563..2042
                     /locus_tag="PLB46_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75253.1"
                     /translation="MLRTRSEIAIVDTFSYDIFEIPFGYTAVEYRWMAKNEGMPVLQI
                     NSQIIFGFETITQVTYKTADVIDGIDNVATNENNFVVYPNPSSTSATIQFTTPENGKY
                     VISISDLQGKIILEKNISALNGQNSIATDLQSFAAGTYLVSLFENNQLIASQKIIKN"
     gene            2045..2455
                     /locus_tag="PLB46_01705"
     CDS             2045..2455
                     /locus_tag="PLB46_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75254.1"
                     /translation="MNKVIITLFIFTTFLTACKTEINETNVSGVWKAKEFESTIPGIP
                     AEYQEAGKKEFLSSVYTLNTDNSLEVHSDFFNAGAKGHWELDTEKKEVAMYYEMDTIK
                     GVETYRILSLEKTTMVLQQDMPDMNGYVKITLQK"
     gene            complement(2533..3237)
                     /locus_tag="PLB46_01710"
     CDS             complement(2533..3237)
                     /locus_tag="PLB46_01710"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRF75255.1"
                     /translation="MKKTTIFTLVGAIALTFTACKNAPDSDEAKTGDAKEVSITEGAA
                     TVAINTTDSKVEWIGTKLSTYHSGTVNIKSGDLQLKDGVIAGGKFVMDMPTLISLKDD
                     ATTNGKLTGHLLSPDFFDVATYPESTFEITGVKPFTGATATGGDQAEISEYTVTDPNF
                     MVSGNLKIKDVTKNIEFPAKITVNNNGVDAVAKFNIDRKLWNIVYPGMPDDLIQDMIW
                     FGISIKASAAEPTALK"
     gene            3475..4248
                     /locus_tag="PLB46_01715"
     CDS             3475..4248
                     /locus_tag="PLB46_01715"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRF75256.1"
                     /translation="MLSMLLISAGLIAVFMTAVWLLSLYKQDMSLVDGFWGLGFVAVT
                     HHWFFRLDEITPKQFVIAYLITLWGLRLAVYIFYRNRGKGEDPRYVAFRNDWKEKTWY
                     ISFYKVFMLQGFLQFVLLLPIIFIMYNGDEKLSVINCIGVLLWTLGWIFETAADYQML
                     RFKKNPANKGKVMDKGLWYFSRHPNYFGEVCVWWGIFLISMNTEWQTFIGILSPILIT
                     YMLLKVSGITMLEKRYEGNDAYSVYKRTTNPFLPWFKKK"
     gene            complement(4252..4902)
                     /locus_tag="PLB46_01720"
     CDS             complement(4252..4902)
                     /locus_tag="PLB46_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HRF75257.1"
                     /translation="MKKLIKRLFIIACVGFFVGCLTILLINRSVNKSAEGKIYTDSNT
                     IPAKDVAVVLGTSRWLTNGYENNYFTYRINAAASLYLNGKVKKILVSGDNGTTSYNEP
                     FEMFDALVDKGVNPDDIVLDYAGFRTFDSMIRANKVFGLNNFIVVSQDWHCERAIYIG
                     QSQNISMIGFAAKDPNISTKTQLREYPARVSAFIDCYVVKNQPKYLGEKIEVIASK"
     gene            complement(4999..6003)
                     /gene="asd"
                     /locus_tag="PLB46_01725"
     CDS             complement(4999..6003)
                     /gene="asd"
                     /locus_tag="PLB46_01725"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRF75258.1"
                     /translation="MRIAVVGATGLVGQMMRRVLEERNFPVTELIPVATERSVGKKVK
                     FRGADVEVVSMETALTMNPQIALFSAGGQTSLEWAPKFEAIGCWVIDNSSAWRMNENC
                     ALVVPEINGDAIKNYPGKIIANPNCSTIQMVLALNPLHLKYKIKRIVVSTYQSVSGSG
                     AKGIMQLHQERSSGCRVVENPAYKHQIDKNVIPQVDVFLENGFSKEEMKMHFETQKIL
                     RDNHIQTSATTVRVPVSGGHSESVNVEFENEYDLQEVYNLLQHTEGVVVQDDLENSIY
                     PMPLDAEGKDEVFVGRIRRDYTQPKTLNCWIVADNLRKGAATNAVQIALYLLANNMVQ
                     "
     gene            6438..8252
                     /locus_tag="PLB46_01730"
     CDS             6438..8252
                     /locus_tag="PLB46_01730"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF75259.1"
                     /translation="MRIILNTILFVLSGLVLTTIFSCSSKNDELDTSAMASIIYTYRI
                     NNHLDSAVLYCADIYNSALEEENLSLQGIIYETVGIANLEAENYHKAIDYFNQAILKY
                     TDIGKEDEADNLLSIINDTSFKTGANTNDTLSFHQNLINNFRIELSGAPSQEEAYLLF
                     VDMLNASLINKKTDVAYRVYVKFKELNSRAGISNYYYQSILLLAQCYWDMGLGDSIIN
                     LSKDILQTQNSYEFDPQQMENCHIIYWLSNILSGKKIVNVELTDKEEVKLIQAFLNDS
                     TYKKILDYGVINLNSTNPISNSDILAVAQLLDKKQVTYRHSLNLALNKFEFKELTWEY
                     LAAGKKTATMRLTFVIILFIITVGALSGYTLFLRWKSYNQIKENNANLRIQVMENEMT
                     QYELNLLKTIRDVQTAERRKVAMDMHDGITNTLTGLSLVATNMQMEENKLTTTNKESW
                     VFMEGNLKKVIQELRTFTHALNDTSYLPNGFKNAIYDYFQVYKKGANMSIKEDGAEMC
                     LVLSPKKQYQLFRIIQELTTNAIKHAGANNITILFCQEDQTIKVKYQNNITLKNGNSA
                     VSGIGEHSIAFRLKELASFDMYQNKQEDLFELTFSLPV"
     gene            8396..10834
                     /locus_tag="PLB46_01735"
     CDS             8396..10834
                     /locus_tag="PLB46_01735"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF75260.1"
                     /translation="MKTTLFTFISFLLLVAFNFTNESQLNQAQDAEVSIDTTKVDFNN
                     LFDIVDSIGQKDTAYCFSLLDSFYQLAATQNDYLAIAKIHNQRAVLNFKLLNINRAEI
                     EIAKAISYAPLLGDNKILVEFYNNSGVIAVQSGTLIAAEEDYKKAIQLADKIQYPEGI
                     IRSVKNLIHLYRIKDDPDEALNILSKYQRVFISEPTQKGRYFESIGEIFNTQENLDSA
                     QYYYKKALTNYILNKNDFQIASIYAKMSFTEIIQGNYETAMEYLEQSDSFAIVSQNEN
                     VKLINTKYYGDLFIRIGDYPQAIEKLDLGISLSKQRGAQKHTVSLLSAKEIALRGNKS
                     YESAIAMNQELISLCAAIGDSVSLVEQYNNLSMNFAELGILDSALYFANIFVKKKKEM
                     GASDLDRAYQTIMVLLIKLEDFENAEINGKMALSYAIHIKDAVRCIGLLESLIEINLK
                     LDDFKDAYLYSNDLLNYKNDLNNDKNLEIANNLKIQYQTSKKEDSIKLLKISEHQQKL
                     INKSRLDELTLANYHRRILIDSIKIGDLKLDSMNNALFKEEIINMESADKIEKMTMQV
                     NYQATVNKYLILLGSASLILIIFIFYYFSYKRKQIYRFNKLELENLLSFMQINPHAIS
                     NSFKAITAVYAGDATLGEKFANQLQVLYRKQFELAHTEDHTLTEELAIIKSYLDTTLL
                     TYNQKVSHNIKILNKFDTDDVLVPSLILLPIVENAVTHHTPNSTLECIVDITLLLKRD
                     TILCNVVDNNDMIIPPNYFEVETKKKRPSGLSITKKRIEILCKLSKKKGYLNFRKTNN
                     LNEVTIEFPIKRIL"
     gene            10831..11655
                     /locus_tag="PLB46_01740"
     CDS             10831..11655
                     /locus_tag="PLB46_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF75261.1"
                     /translation="MSTIKCIIVDDDFECIKHMTSVLIQFEKEFKIISVAKSYEEAVF
                     LIDTNKPDLVFLDIDLRSEKNGFDVLYNTKFKNFDVVFTTRFDEFMIQAIRCSAFDYL
                     LKPIEFEDIEKLLIRIKTEKKQALSFSTLFANLVFVEQGEVQIIRLPILWQEYLQSFE
                     LNSIEAFVTTENKYAKALYYVPQSPEGINFVKQHQLKKELKTSDFIYSSATISKGVKQ
                     IKREYLIASPQFMNINQKTLINTKSIIRCTRDGAYVTTKGELRFPVSPDFRAGFKF"
     gene            complement(11945..13489)
                     /locus_tag="PLB46_01745"
     CDS             complement(11945..13489)
                     /locus_tag="PLB46_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75262.1"
                     /translation="MPELLHNAYLLVSGLNKSELQYVTSQIKKLPKRYQQTDGFRLFF
                     TIVDIKQYNEEEIRYKWWRKAGIKKDKRFINAAQKEMIDFVSENLIKYNKLSKDFHYE
                     KSLMIGTVLAKRKIYNAAYYYYKESLEALSVSDQPYNRAHVLHQMFIVFPHIQIPNKN
                     LELRNLIQIEKEIVKDISLFYKAFRLFMHYVPLIVKQVIPQGEKERNKIKEILNLDVL
                     KQDYTGASLFTIIYINKLLSMAHKVLGNIEERLYYQQIIIQQLNLERKLVLKHIPALY
                     IQEIFDFADASLSSNKFSDANLAIIDGETLLNNLEENTIEKKAICLHLKLSLALKQKN
                     SKLVASHLDSLNSYYQTESNNINNNFKTAFLFSMLKGSYFIKDYKLLNITYNAINQLN
                     KEFMLDIFKIASLIYLAATYEQFERHHNNLNITMPNIFINAVQKSKPLFRENKYKYRL
                     TIIIFKHIARLSESNKIIEHNTIFTNLNNELETINEKELIFKTQLLSIWDYNSWIKEI
                     LLKLKI"
     gene            13772..14731
                     /locus_tag="PLB46_01750"
     CDS             13772..14731
                     /locus_tag="PLB46_01750"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HRF75263.1"
                     /translation="MKYTFTYLIFQFIVLCSFAQNEIKSDKSNDKVTIENLNDTCDSA
                     LPLQNRLRFSVTSFNINTVSVPNTLGENMATMLTNGLQNTNCFRVLQSVNGMDDINAV
                     NAFNNSNDSKSSFLKEQFIVTGEITEYSKQDKDKSIAGVKVGKDVVKLGFIIQILDPG
                     TRDVLFSNSFNIEGKASTGGSFGVTVPYMGRINLAEATNNDPALADAMENGIMQAVSY
                     IASNKHKIPLPDETAANINTSILTITGITFNQINEIDAWIKLDTQCVVTKKGSFENGV
                     MTFTVTHRNSVYDLLTNLTANSQNKIQVLEVGNTGPVKLTILK"
     gene            14737..17430
                     /locus_tag="PLB46_01755"
     CDS             14737..17430
                     /locus_tag="PLB46_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75264.1"
                     /translation="MKKLLLSAYTLLFVLVAKGQDDVPNNTKDAAAVIAAPLVFTGSL
                     SPAADVDYYKLVVETAGVYEFDVFVSGTSLDVVFYLTDYSESKIYYSESNSGGNDVNK
                     DVLLCAGEYLIKVYEDNGETFADTYEFNLNAVTNDQCECNNTQADAFPISTDTTVNFM
                     LYGYNSYYGEINYDVDYFLLNITEGGVLTTEVVNNTLDSEIDFTIFDLDSNEVHGYHP
                     TSGGGVDITKSTLLCPGEYYLRIKDGGDGSEMSTELNVLNLTFNSSDIYECNNSQPTA
                     SEIALDSTISFSIHGYNYLIEEPGASSDDVDYFKTWFDSTGVLKLFFQNNTDESPFFV
                     QVLNEDGIEIWSTTSSLDITYYAQVCAGMYFIRIQEDDNFGGLENSPALNNLTISFIN
                     DEFECNNEYESAYPIGVNDTLSFTILGKNKLYEIDNDRDYFKFTPDCDGILSLGLYNA
                     TSGVQVYARVIEDFFAFPVEKTLYAGAYGVLDYDTVHLLGGQQYFIEVYDSGDNNQSE
                     GLIQLGLNYTTFVTDSILIDGSLSFCSGEGEVLLTAEQPGATYLWNTGATTQSIAVTS
                     GGWYAVTVEVDECSLYSDSIFVEELIAPVVTLSETDTAVCTYELPIIVSAYGAETYEW
                     STGETDEDIVITSAGVYYVTGTGLNGCSSVSEDLIVEVNEPPMVYITPLFTDTIICDD
                     DVLTISALEGPGYTFNWSTGSTENTIEVEDSGIYTVIITNANGCISESDELNVSVAPT
                     PETPTINLVGTQLQCSVIGSAYQWYFDLTPIPTATEQNYTPTENGTYRVEVYNLFNCS
                     AISEPFSFTLDGINSNEKPFNVFIWPNPATNSIFISFDENTFEGSSIYLYNVEGKRIF
                     ENKLINSKLFEIDITNFPTGLYQLVVQNEEYVIMENVLIIE"
     gene            complement(17506..18189)
                     /locus_tag="PLB46_01760"
     CDS             complement(17506..18189)
                     /locus_tag="PLB46_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF75265.1"
                     /translation="MNPITLAIADDHELVRKGLKLLLNSFEHFNVIHEAPNGKQLIDW
                     LNTTPSHPNIALVDVGMPIMNGFETTSYIHSEFTNVKIVALSVYDDEQTILKMIESGA
                     NAYLLKDSAPELVKETLLQVNEKGYYYSQHVMNAMLKAKDQKFGGNHSTANRNTIQPI
                     DTLTAREIEFIKKCCSEKTYKEIASEMKISQRTVDGYRESVFSKLDIKSRSGIVVFGF
                     MNGLVENKG"
BASE COUNT         6109 a         2909 c         3265 g         5967 t
ORIGIN      
        1 attggtaaca ccgtcagctg gtgagaccgc acctaacgag cccgacaggg cggcatattg
       61 gtttgccaga acgcaaggtt gattgttttg cttgcgtgtt tgccgcgtag gctactgtcg
      121 gatttgattt tttatcgatg gatgggatat tgtataagca ttggattttt tgatgcgttt
      181 aaccaatgaa taagtttgag catgttttct ttactcattg ccgtgcatcc gcttgttggt
      241 gtatttggtg ttttataaat atgtaaaaaa atgcatgagc cgtttcccgg aacttgcgga
      301 ttggtattat aatgtaccca aacaccatat tcatattgat gatcatcgcg gcgcatatat
      361 tcaaaactgt taaagtcttt ttctaaaatg gtatctacat caattaacgt attgtaatat
      421 tttgaattaa tatcatctac acataaatca cttttattca cttgtttaaa aggcatgttt
      481 aagttgtcta aagcatgatt actaaaaacg ggacctaatg aaaaaattcc cgaaggcgca
      541 catccatcac cttcttgttt aatagggtcg tttgttgttt gttgccagct tgaattttta
      601 tcgcgcgcca ttccgtttcg gccaaccata acatcaaaag tatcgacaac ccgccagtca
      661 gatgccgtgt cagcgcgttc aagtaactgc atagtgccgg acgttgttga ccaatcatgc
      721 gtttgcacta caattaattg aaggccattt accggataag atacagcggc agcgggtttg
      781 tttttgggtt tgttacaagc tcccatgctg aatatgatga gaattatggc aaatttgtta
      841 aacataatgc aagatataca gtctgtctca ttgttgcaat atatgcacgt ttgtcctgta
      901 gcagcatgat gcaggttgta acttaaacac tgcattcgcc tactttcgta tctttgtaat
      961 aattcaataa catgaaaaaa tttttatcgt ttgtattagc ttgcacactc gcaactttta
     1021 catttagtca gtcccccatt gttttagaag aatctgattt tgcaacaggt ggcgattcat
     1081 attatatcgt taacgcaaat ccggcaatta cttttgacgc cgaagcaacc ggtccggatt
     1141 acaattggga ctttactgat ttagaagtaa tgactgctac agtgactaat tttgaagacc
     1201 caacagatac cgacccatta tatttctttt tatggttgtc gtcggatgtt gcacaacaaa
     1261 atccaaccga tttagccaac gactttatta ctattgaaga tgtattcaat ttttataaac
     1321 tgaataatga tgaatttgcg caaaccggat ttgcaggaac aatttctgat attcctttgc
     1381 cgatagctta tgataataat gaaattttat accaatttcc atctgcatat tccgaagatt
     1441 agtcagatga agcaggtttt ggtattacag tccccggttg tgggggttgg agcgaatttc
     1501 gcggtcgcac caacgaaaac gatggctggg gaactattac aacacccgcc ggggaatttg
     1561 aagtgctaag gacaaggtca gaaattgcta ttgtagatac cttttcgtac gatatttttg
     1621 aaatcccttt cggatatacc gcagttgaat atcgctggat ggcaaaaaat gaaggcatgc
     1681 cggtattaca aattaattcg caaataattt ttggatttga aacgatcact caggtaactt
     1741 ataaaacagc ggatgtaatt gatggcattg ataatgtagc aacaaatgaa aataattttg
     1801 tagtatatcc aaatccttca tcaacttctg caactataca atttactaca cctgagaatg
     1861 gaaaatatgt aattagcatt agcgatttac aaggaaaaat aatacttgaa aaaaacatta
     1921 gcgcactaaa tggtcaaaac agtattgcta ctgatttgca atcatttgca gcaggaacat
     1981 atctggtatc gttatttgaa aacaaccaat taattgcatc tcaaaaaatt ataaaaaact
     2041 aataatgaat aaagtaataa taaccctctt catatttacc acatttttaa cggcttgtaa
     2101 aaccgaaatt aatgaaacca atgtttcagg tgtatggaaa gctaaagaat ttgaaagcac
     2161 cattcccggc attcctgcgg agtatcaaga agcggggaaa aaagaatttt tatcttccgt
     2221 gtatacatta aacaccgaca actcattaga agtgcacagc gattttttta atgccggcgc
     2281 aaaaggacat tgggaattag ataccgagaa aaaggaagtg gccatgtatt acgaaatgga
     2341 taccattaaa ggcgttgaaa cctaccgcat tttatcactt gaaaaaacta ctatggtatt
     2401 gcaacaggat atgccagata tgaatggtta tgttaaaata actttgcaga aataacgtta
     2461 agacctgata taaaatgcaa tgccggaaag tttacactcc ccggcatttg aaaaacaacc
     2521 actactctgt ttttatttta acgctgtagg ttcagcggca ctagctttta tagaaatacc
     2581 gaaccaaatc atatcctgaa ttaaatcatc aggcatacca ggataaacga tattccacag
     2641 ttttctgtcg atgttaaatt tagcaacagc atcaacacca ttattgttca cggtaatttt
     2701 tgccgggaat tcaatatttt ttgtaacgtc tttaatttta aggtttccgc taaccataaa
     2761 gttagggtca gtaacagtat attcactgat ttcagcctga tcaccaccag ttgcagttgc
     2821 tcctgtaaat ggtttaacac cggtaatttc aaaagttgac tcagggtaag ttgcaacatc
     2881 aaaaaagtca ggtgacaaaa ggtgaccggt taattttcca ttagttgttg catcatcttt
     2941 taacgagatt aatgtaggca tgtccattac aaacttaccg cctgcaataa caccatcttt
     3001 taattgtaaa tcgccacttt taatgtttac agtaccgcta tggtaagttg aaagtttggt
     3061 gccaatccac tctactttgc tgtctgttgt attaatggca acagttgcag caccttctgt
     3121 gattgatact tctttagcgt cgccggtttt ggcttcatcg ctgtccgggg catttttaca
     3181 tgccgtgaaa gtcagtgcta tcgcaccaac aagggtaaaa attgtcgttt ttttcatcat
     3241 tttttgtttt atatgtatat acatacattt acagaaacag caaaattgca aaaaagttca
     3301 acatttggat aaaaatgtcg gaaacggcct aaaaacggca tttggaggtt gtgacctgaa
     3361 ggttttggca aattgatggc aagggttaat ggcaatcagg caaaattggg aatattccct
     3421 aattttaaac actttgcagc acatttcgtt ttaccatcat tcaaattctc ttttatgcta
     3481 tcaatgttac ttatttctgc cggattaata gctgtattta tgacagctgt ttggttgttg
     3541 agtttgtata aacaggatat gagtttagtg gatggatttt gggggttagg atttgttgca
     3601 gtaacacatc attggttttt tagattagat gaaatcacgc caaaacaatt tgtcatcgcg
     3661 tatttaatca cgctttgggg attacgttta gcggtgtata tattttatcg caacagagga
     3721 aagggcgaag acccaagata tgttgcattt agaaacgact ggaaagaaaa aacctggtat
     3781 atcagttttt ataaggtatt tatgttgcag ggatttttgc aatttgtttt attgctgccc
     3841 attattttta ttatgtataa tggtgacgaa aaattgagtg ttattaattg tatcggtgtt
     3901 ctactttgga ccctgggatg gatttttgaa acagctgccg attatcagat gttgcgattc
     3961 aaaaaaaatc ctgcgaataa aggaaaggtg atggataaag ggttgtggta ttttagtcgc
     4021 caccccaatt atttcggaga agtatgtgtt tggtggggaa tatttttaat cagtatgaat
     4081 acagaatggc aaacattcat tggcatttta tcgcctatat taattaccta tatgttatta
     4141 aaagtttcgg gcattaccat gttagaaaaa cggtatgaag gaaacgatgc gtattcagtt
     4201 tataaacgca caacaaatcc gtttctgcca tggtttaaaa agaaataata attatttact
     4261 ggcaataact tcaatttttt cacccaagta tttgggctga ttttttacca cataacaatc
     4321 gataaaagca ctcacgcgag ccggatattc gcgcaattga gttttggtag agatattcgg
     4381 gtctttagca gcaaaaccta tcatgctgat gttttgtgat tggccaatat aaatagcccg
     4441 ctcacaatgc caatcctgtg aaacaacaat aaaattattt agcccaaaca ccttattagc
     4501 ccttatcatg ctgtcgaaag tgcggaaacc cgcgtaatcg agcacaatat cgtcggggtt
     4561 tacaccttta tccaccagtg catcaaacat ctcgaaaggc tcgttgtaag aagtggttcc
     4621 gttgtcgcca cttaccagaa tttttttcac ttttccgttc aggtacaaac ttgcagcggc
     4681 attaatacga taggtgaaat aattattttc ataaccatta gtcagccaac gggaggtgcc
     4741 taaaacaaca gcaacatcct ttgccggaat agtattgcta tccgtataaa tttttccttc
     4801 agccgattta tttacggagc ggttaatcaa taatatggta aggcaaccga caaaaaaccc
     4861 gacacatgcg ataataaaaa gacgtttaat taattttttc aagtgcgtta ttaattcatc
     4921 aaaattaaaa aaaagccgaa atgatgaata atatcattaa cggctttaat cacaatttat
     4981 ttgtttacag tataaaaatt actgcaccat attatttgcc aataaatata aagcaatttg
     5041 cacagcatta gttgcggcgc ctttgcgtaa attatctgca acaatccagc agttaagtgt
     5101 ttttggctgt gtatagtcac gacgaatgcg gccgacaaaa acctcatctt tgccttcagc
     5161 atctaaaggc attgggtaga tgctattttc aaggtcatct tgcacaacaa cgccttcggt
     5221 atgttgtaac aggttatata cttcttgtaa gtcgtattca ttttcaaatt ccacattcac
     5281 actttcacta tgaccgccgc ttaccggaac acgaacagta gttgcagagg tttggatatg
     5341 attatcgcgt aatatttttt gtgtttcgaa atgcattttc atttcctctt tgctaaaacc
     5401 attttccaaa aacacatcca cctgcggaat tacattttta tcgatttgat gtttgtaagc
     5461 aggattttca acaacacgac aaccgctgct gcgttcttga tgtaattgca taattccttt
     5521 tgcaccacta cctgaaacag actgataggt agaaacaaca atacgtttta ttttgtattt
     5581 aagatgtaat ggattgagtg ccaacaccat ttgaatggta gaacaatttg ggttagcgat
     5641 aattttaccc ggataatttt taatggcatc tccattaatt tcaggcacta ccagcgcaca
     5701 attttcattc attcgccagg cgctggaatt gtcgattacc caacaaccaa ttgcttcaaa
     5761 tttaggtgcc cactccagac tagtttgacc gcccgccgaa aacagtgcaa tttgagggtt
     5821 cattgtaagg gctgtttcca tgctcaccac ttcaacatcg gcaccgcgaa acttcacttt
     5881 tttaccaacc gaacgttcgg tagcaacggg aattaactcg gtaaccggaa aattcctttc
     5941 ctccaaaacc cggcgcatca tttggcccac aagccctgta gcacccacaa cggcaattct
     6001 catgattttt ttttgcaaag gtaagggaaa agtggaattg gcgaatagtt cacatggggc
     6061 aacaatcaaa tgtttaattc gtgaggggaa agtaggggtg atagtggcgg tttggttaaa
     6121 atggcacaag gttaatttgc tgtttccttt taaaattata ccacctgaat ataatccaag
     6181 cctactttag atgattaaaa attaatatgc aaatgatcaa aaaaagctat atttcattgt
     6241 tagaataaag aatagataat aaaaagcctt tatatgtttt aaaatggcat gtgtttaata
     6301 cttagtattg aaagagagtt tataacatta aaatgagagg caataaggat gtgtaaatgt
     6361 taaaaaaaag gattattaac agagattgag tttctaaaaa atgaaaatat aagcttataa
     6421 tttttataaa aatatatatg aggataattt taaatactat actttttgtt ttaagtggat
     6481 tagtactaac caccatattt agttgtagtt ccaaaaatga cgaattagat acttccgcga
     6541 tggcatcaat aatttatact tatagaatca acaaccattt agatagcgcg gtattgtatt
     6601 gtgcggatat ttataatagc gctttagagg aggaaaattt atcgctacaa ggaattattt
     6661 atgaaacggt tggtatcgcc aatttggaag ccgaaaacta tcacaaggct attgattact
     6721 ttaatcaggc tattttaaaa tacacagata tcgggaagga agatgaagca gataatttat
     6781 tatcaattat taatgacact tcattcaaaa cgggtgcgaa tacaaatgac accctatctt
     6841 ttcaccaaaa cttaataaat aatttccgta ttgaactttc aggagcaccc agccaggagg
     6901 aggcatacct actatttgtt gatatgctta atgcatcttt aattaacaaa aagacagacg
     6961 tggcataccg agtatatgtt aaattcaaag aactaaattc ccgagcgggt atttctaatt
     7021 attattatca atctatttta ttgttggcac aatgctattg ggatatgggg ctcggcgatt
     7081 caataattaa cctttcgaaa gatatccttc aaactcaaaa tagttatgaa ttcgatccgc
     7141 aacaaatgga aaattgccat attatttatt ggttgtcaaa tattttgagt ggaaaaaaaa
     7201 ttgtaaatgt agagcttaca gacaaagaag aagtcaaatt aatacaagct tttttaaacg
     7261 atagcacgta taaaaaaata cttgattacg gagtaattaa tttaaatagt accaatccaa
     7321 taagcaacag cgatattcta gcggtagcac aactactaga taaaaaacaa gttacgtaca
     7381 gacattcatt gaatctggcg ttaaataaat ttgaattcaa ggagttgacc tgggaatact
     7441 tggctgctgg caagaaaact gccacgatgc ggttaacctt tgtaattatc ctatttataa
     7501 taacagttgg agccttgtca ggatatactt tgttccttag atggaagagt tataatcaga
     7561 ttaaagaaaa caatgccaac cttcgaattc aagtaatgga gaatgaaatg acacaatatg
     7621 aattaaattt attgaagact attcgagatg tgcaaacggc tgaaaggcgc aaggtggcaa
     7681 tggatatgca cgatggaatt accaatactt taacaggatt aagcctagtt gccaccaaca
     7741 tgcaaatgga agagaataaa ctaactacta caaataaaga atcgtgggta ttcatggagg
     7801 gtaatttaaa aaaagtaatt caagaattgc gcacatttac acacgcgcta aatgatacaa
     7861 gttatttacc aaacggattt aaaaatgcca tctatgatta tttccaagtg tataaaaagg
     7921 gtgcaaatat gtccataaaa gaagatggtg ctgaaatgtg tttagtcttg tcaccaaaaa
     7981 aacaatatca attattccgt atcattcaag aactaactac taatgcaatc aaacatgcag
     8041 gagcaaataa tattactatt ttattttgtc aggaagatca aactataaaa gtaaagtatc
     8101 aaaataatat tacattaaag aatggcaatt ctgctgtaag cggaattggt gagcattcaa
     8161 ttgcatttag attaaaagaa ttagcaagtt tcgatatgta tcaaaacaaa caggaggact
     8221 tgtttgagtt gacgtttagt ttgcctgttt agatggtact tttaacaaat gaatatacat
     8281 ttggttgtca atacacaaat atttattttt atatgctcct caagtactat taatcgaacc
     8341 ccttttgatt catagtaaca aattttgttt tattatattt taataaataa caaatatgaa
     8401 aaccacatta tttactttta tttccttttt gttacttgtt gcttttaatt ttactaatga
     8461 aagtcaatta aaccaagcac aagatgcaga agtttcgatt gacacaacaa aagtggattt
     8521 taataattta tttgatattg tagattcaat tggacaaaag gatactgctt attgtttttc
     8581 acttttagat agtttttacc aactagcagc aacccaaaat gattatttgg ctatcgctaa
     8641 aattcataat cagcgcgcag ttttgaattt taaactgcta aacatcaata gggctgaaat
     8701 agaaattgct aaagcaattt catatgcacc cctgttaggg gataataaaa ttctggtaga
     8761 gttttataat aatagtggtg taattgctgt gcagtccgga accttaattg ctgcagaaga
     8821 ggactacaaa aaggctattc aactagctga caaaattcaa tatccggagg gaataatacg
     8881 ctcagtaaaa aatttaattc acttatatag aataaaggat gatccggacg aagctttaaa
     8941 tatattgtct aagtaccagc gtgtttttat tagcgaacca actcaaaaag ggcgttattt
     9001 tgaatcaata ggggagatat ttaacacgca ggaaaacctg gattctgcac aatattatta
     9061 taaaaaggct ttaacaaatt atattttaaa taaaaatgat tttcaaatcg ctagtatata
     9121 tgctaaaatg agttttactg aaataattca aggcaactat gaaacggcaa tggagtattt
     9181 agaacaaagt gattcctttg ctatcgtgag tcaaaatgaa aatgtaaaat taataaatac
     9241 caaatattat ggcgatttat ttatacgtat tggcgactat ccccaggcta ttgaaaagct
     9301 tgatttgggt atctcacttt caaaacaacg aggcgcccag aaacatactg ttagtttatt
     9361 aagtgcaaaa gaaatagcat taaggggtaa taaaagttat gagtcagcta ttgcaatgaa
     9421 ccaagaattg atatcacttt gtgctgcaat aggagacagt gtttctttag ttgaacaata
     9481 caataacctc agtatgaact ttgctgaatt aggcatctta gacagcgcat tgtattttgc
     9541 aaatattttc gttaaaaaaa agaaagagat gggcgctagt gatttggata gggcttatca
     9601 aacaataatg gttttattaa ttaaattaga ggactttgaa aatgctgaaa ttaacggaaa
     9661 aatggcttta agctacgcta tccatattaa agatgcagtc agatgtatag ggttacttga
     9721 gtcattaatt gaaattaatt tgaaacttga tgactttaag gatgcctacc tttattctaa
     9781 tgacttattg aattacaaga acgatttaaa taatgataaa aatctagaaa ttgctaataa
     9841 tttaaaaatc caatatcaaa cgagtaagaa agaagattca attaaacttt taaaaatcag
     9901 cgaacaccag caaaagctta taaataaatc aagattagac gaattaacat tggcaaatta
     9961 tcatagacgg attttaatag attctattaa aattggtgac ttaaaattag acagcatgaa
    10021 taatgcgctt tttaaggaag aaataattaa tatggaatct gctgataaaa tagaaaaaat
    10081 gacaatgcag gtgaattatc aggctactgt gaataaatac ctaatacttt tagggagtgc
    10141 gagtttaatt ctaattattt ttatatttta ttactttagt tataaacgta aacaaatcta
    10201 ccgttttaac aaactcgaat tagaaaactt gctatcattt atgcaaataa atccacatgc
    10261 aatatccaac tcttttaaag cgataacagc ggtatatgca ggagatgcaa cacttggtga
    10321 gaaatttgct aatcaattac aagtgctata tagaaaacaa tttgaattgg ctcatacaga
    10381 ggaccataca ttaacagagg agcttgctat tattaaatca tacttagaca caactttgtt
    10441 aacttataat caaaaggttt cgcacaatat taagatttta aataaatttg atactgatga
    10501 tgtattagtc ccatctttaa tattattacc aatcgtggaa aatgctgtaa cacatcacac
    10561 acccaactcg actttagaat gtatagtcga tattactctg ctacttaagc gagataccat
    10621 tctatgtaat gtagttgaca ataacgatat gattataccc ccaaattact ttgaagttga
    10681 aactaagaaa aaaagaccca gcggtttgag cattacaaaa aaaagaattg aaatattatg
    10741 taaattaagc aagaaaaaag gataccttaa tttcagaaaa actaataact tgaatgaagt
    10801 aaccattgaa tttcccatta aaagaatatt atgagcacga ttaaatgtat tattgttgat
    10861 gatgactttg aatgtatcaa acacatgact tctgtgttaa ttcaatttga aaaggaattt
    10921 aaaattataa gcgttgctaa aagttatgaa gaagctgttt ttttaataga tactaataaa
    10981 ccggatttgg tgtttttaga tattgattta agaagtgaaa aaaatgggtt tgatgtattg
    11041 tataatacta agtttaagaa ctttgatgtt gttttcacta cacggtttga cgaatttatg
    11101 atacaggcaa taagatgtag tgcctttgat tatcttttaa aaccaataga atttgaggac
    11161 attgagaaac tgttgattcg aattaaaacc gaaaagaaac aagcgttatc cttttcaaca
    11221 ttatttgcaa atttggtttt tgttgaacag ggtgaggtgc aaattattag gctacccata
    11281 ttgtggcaag aatatttgca atcattcgaa ttaaatagta tcgaagcatt tgtaacgacg
    11341 gagaataaat atgcaaaagc tctttattat gtacctcaat cacctgaggg aataaatttc
    11401 gttaaacaac atcaattgaa gaaagaatta aaaacttcgg attttatcta ttctagtgcc
    11461 acaatttcaa aaggtgttaa gcaaataaaa agagaatacc ttatagcctc gccacagttt
    11521 atgaatatta atcaaaaaac attgataaac acgaagtcaa ttattagatg cacaagggat
    11581 ggggcatatg tgactacaaa aggggagctg aggttcccag tgtcaccaga ttttcgagcc
    11641 ggctttaagt tttaattaca gtaggattaa taatccggga tataccggtt ttactccagt
    11701 tttagcctag tttacacgaa ttgctgttta ccggtgactt taaacgctaa ttttattttc
    11761 atgtggtgac tgaaagtaat ttcagtttca cagaattaag aaatgtattc agttaacagt
    11821 aaaacacaat tgaaatgtgg acttttatta ctgctctcag aatttgggag tgggtttggc
    11881 ctaattagtt agaccgtcaa cttgaaaaca aatgcttgac ataggcgcca caagcacttg
    11941 tttattaaat tttaagtttt aataaaattt cttttatcca agaattatag tcccaaattg
    12001 acaacagttg tgttttgaaa attaattctt tttcgtttat cgtttctaac tcattattta
    12061 aatttgtaaa tattgtatta tgttcaataa tcttgttgct ttccgaaagc cgtgcaatgt
    12121 gcttgaaaat tattattgtc aagcgatatt tgtatttatt ttcccgaaat aaaggtttac
    12181 ttttttgtac tgcgttgata aatatatttg gcatggtaat gtttaagtta ttgtgatgtc
    12241 gctcaaattg ttcataagtc gctgctaagt atataagtga agctatttta aaaatatcta
    12301 acataaactc tttattcaat tgatttatag cattataggt tatattcaat aacttgtaat
    12361 ctttaataaa ataacttcct tttagcattg aaaaaagaaa agccgtttta aaattattgt
    12421 ttatattatt actttcagtt tgataataac tgttgaggct atcaaggtgg gacgcaacca
    12481 gttttgaatt tttttgtttt agcgcaaggc ttagtttaag gtgtaaacaa attgcttttt
    12541 tttctattgt gttttcttct aggttattca aaagtgtttc gccatcaatt attgccaaat
    12601 ttgcgtcaga aaatttattt gaacttaaac tggcgtctgc aaaatcaaat atctcttgaa
    12661 tataaagggc aggtatatgt tttaatacta attttctttc taaatttaat tgttgaatta
    12721 ttatttgttg atagtaaagt cgctcctcaa tattgcctaa tactttgtga gccatagaaa
    12781 gaagtttatt aatataaatt atagtaaata atgaagcgcc ggtgtagtct tgttttagca
    12841 cgtcgagatt aagtatttct ttaattttat ttcgttcttt ttcgccttga ggaataactt
    12901 gttttactat taagggcacg taatgcataa atagccgaaa agctttgtaa aaaagtgata
    12961 tatctttaac gatttctttt tcaatttgaa ttaaattacg taattctaaa tttttattgg
    13021 gtatttgaat gtgcggaaaa acaataaaca tttgatgtag aacatgtgca cggttatagg
    13081 gttgatcact tactgataat gcttctaagg attctttata gtagtaatag gcggcattat
    13141 atatttttct ttttgctaaa accgttccaa tcattaacga tttttcgtag tgaaaatctt
    13201 tcgataattt attatatttt attaaatttt cagatacaaa gtcaatcatc tctttctgtg
    13261 cggcattaat aaatcgttta tcctttttaa tacccgcttt tctccaccat ttatatctaa
    13321 tttcctcctc attatattgt ttaatatcga caattgtaaa gaataaccta aatccatcag
    13381 tttgttgata gcgtttgggc aatttcttta tttgggaagt tacatactgt aattctgatt
    13441 tatttaaacc gctaactaac aaataggcat tatgtaacag ttcaggcata ttgatttaat
    13501 tatttggtta aaggtattaa aaaaaacgaa gaaaatggta agatgttagt tgtctaaaat
    13561 attgaaaatc aataagatat agaataaata ctaaaaattt cacttacaat ttaataccta
    13621 ttttatattt tattaattta taaaaagcag accttaggta actaaatata ctttttaccc
    13681 aacttagtga acaaggtggt tgattcacac aaatctaatt tgtttggaat acaagcacac
    13741 ttggtttaga gactaactaa attacctgga tatgaaatac acctttactt atttaatttt
    13801 tcaatttata gttttatgca gcttcgccca aaacgagatt aaatccgata aatcgaacga
    13861 caaagttacc attgaaaatt taaacgatac atgtgattct gctttgccat tacaaaatcg
    13921 tttaagattt tctgtaacga gttttaacat taatacagtt agtgtaccca acacgttggg
    13981 tgaaaatatg gcaacaatgt tgaccaatgg actacaaaat acaaactgtt ttcgtgtttt
    14041 acagtcagtt aacggaatgg acgatattaa tgctgttaac gcgtttaata attcaaatga
    14101 tagtaaatca agttttttaa aagagcaatt tatcgttact ggtgaaatta ctgaatattc
    14161 aaaacaagat aaggacaaaa gtattgccgg ggtaaaagtg ggcaaagatg ttgttaaact
    14221 tgggtttatt attcaaatat tagatccagg caccagagat gttttatttt ccaattcatt
    14281 taatattgaa gggaaagcaa gcactggtgg aagttttgga gttacggttc cttatatggg
    14341 gaggattaat cttgcagaag caacaaataa cgaccctgcg ttagcagatg ccatggagaa
    14401 tggcattatg caagccgtta gctacatcgc ttcaaataag cataaaattc cactacctga
    14461 tgaaaccgct gcaaacatta atacctctat attaactata accggaatca catttaacca
    14521 aatcaacgag attgatgcat ggatcaagtt agatactcaa tgtgtagtta ctaaaaaagg
    14581 ttcatttgaa aatggtgtca tgacatttac ggttacacat cgtaattcag tctatgattt
    14641 actgacaaac ttaaccgcta atagtcaaaa caaaattcaa gttttagaag tgggcaacac
    14701 cggacctgtt aaattaacaa tacttaaata aatgttatga aaaaactgtt actttctgca
    14761 tatactttac tatttgtatt agtagccaag ggacaagatg atgtaccaaa taataccaag
    14821 gacgcagctg ctgttattgc tgctccgctg gttttcacag gctctttatc gccagctgct
    14881 gatgttgatt attacaaatt ggttgtcgaa acagctggtg tatacgagtt tgatgtattt
    14941 gtttcaggta caagtttgga tgtggttttt tatttaactg attatagtga atctaaaatt
    15001 tattattctg aatccaattc aggtggtaat gatgtgaata aagatgtttt attatgtgct
    15061 ggagaatact taattaaagt atatgaagat aatggagaga cttttgcaga tacttatgaa
    15121 tttaatttaa atgcagttac taacgaccaa tgcgaatgca ataacaccca agcagatgct
    15181 ttcccaatta gtactgatac aaccgtaaat tttatgcttt atggttataa ttcttattac
    15241 ggtgaaatca attacgatgt tgattatttt ttgctaaata ttacagaagg aggggtttta
    15301 acaactgaag ttgtaaacaa tactctagat agtgaaatag acttcacgat attcgatctt
    15361 gattcaaatg aagttcatgg ttaccaccca acttcaggcg gcggtgtcga tattactaaa
    15421 tctacattac tttgccccgg tgaatattac ttaagaatta aggatggagg agacgggtct
    15481 gagatgtcca cagagttaaa tgttttaaat ttaacattta attcaagtga tatttatgaa
    15541 tgcaataatt cacaaccaac tgcttcggaa attgcattag attcaacgat tagttttagt
    15601 attcatggat ataattacct tattgaagag ccgggggcat cgtcagatga tgtggactat
    15661 tttaaaacat ggtttgattc tacaggtgtt ttaaagctat tttttcaaaa taacacagac
    15721 gaaagccctt tttttgtgca ggttttaaat gaagatggta ttgaaatttg gtctactact
    15781 tcatcccttg atattacata ttatgcccaa gtttgtgcag gaatgtattt tattcgaatc
    15841 caagaggatg ataactttgg tggtttggaa aattcaccag ctttaaacaa cttaaccatt
    15901 agttttataa atgatgaatt tgaatgcaat aatgaatatg agtcggcgta tccaataggg
    15961 gttaacgaca cactaagttt tactattttg ggaaagaaca aattatatga aattgataat
    16021 gatagggact attttaaatt cactccagac tgtgacggaa tattatcgct tggcttatat
    16081 aatgccactt ctggagtcca agtatatgca agagttattg aagacttctt tgcctttcct
    16141 gttgagaaaa cactttatgc aggtgcttat ggtgttcttg attatgacac ggtacattta
    16201 ttagggggtc aacaatattt tatcgaagtg tatgatagcg gagataacaa tcaaagtgaa
    16261 ggtttaattc aactaggact aaattataca acttttgtaa cggatagtat tttaattgac
    16321 ggttcacttt cattttgctc tggagaagga gaagtcctac ttacggcgga acagccaggt
    16381 gcaacatacc tatggaatac cggtgctact acacaatcta ttgcagtaac atctggaggg
    16441 tggtatgcgg ttacggttga ggttgacgaa tgttccttat attcagattc tattttcgtt
    16501 gaagaactaa ttgcaccggt tgtaacccta agtgagacag atacagcggt ttgtacatat
    16561 gaattgccaa taattgtttc agcttacgga gccgaaactt atgaatggtc tacaggtgaa
    16621 acagatgaag atattgtaat aacgagtgca ggggtatatt atgtaaccgg aacaggtttg
    16681 aacggatgtt cgtcagtttc ggaggatttg attgttgaag tgaatgaacc cccaatggtt
    16741 tatattaccc ctctatttac agatacaatt atatgtgacg acgatgtgtt aaccatttcg
    16801 gctttagaag gtccgggata tacatttaat tggagcacgg gctcaaccga aaacactatt
    16861 gaagttgagg attcgggtat atatacagta ataataacaa atgcgaacgg ttgcatttcc
    16921 gagtcggatg aacttaatgt aagcgtagct ccaacgcctg aaacaccaac aattaatctt
    16981 gttggcactc agttacaatg ttctgttatt ggtagcgcgt atcagtggta tttcgactta
    17041 acaccaattc caacagcaac tgaacaaaat tatacgccaa cagaaaatgg aacatatcgt
    17101 gtagaagttt ataatctgtt caattgttct gccatcagcg aaccgtttag ttttacactt
    17161 gatggaataa atagtaatga aaagccattt aatgtgttta tttggcctaa tccggctaca
    17221 aattcgatat ttatatcttt cgatgaaaat acattcgagg gaagtagcat ttatttatac
    17281 aatgttgagg gtaaaagaat ttttgaaaat aagttgatta attcaaaatt atttgaaatt
    17341 gacatcacta attttccaac aggtttatat cagttagtag tacaaaacga ggaatacgtt
    17401 ataatggaaa atgtacttat tatagaataa cgggtagaaa atgatttaag gcagtgcggg
    17461 caattttaaa ttatttataa aattgcccaa catcaaatca tatctctacc ctttattttc
    17521 aaccaaacca ttcataaatc caaataccac gataccgctt ctggatttaa tatcaagttt
    17581 agaaaaaacg gattcgcgat acccgtcaac tgtgcgctga ctgattttca tttctgaagc
    17641 aatttctttg taggtttttt cgctgcaaca cttttttata aattctatct cccttgccgt
    17701 taatgtgtca ataggttgaa tggtatttct atttgcagtt gaatgatttc caccaaattt
    17761 ttggtctttg gcttttagca tagcgttcat cacgtgttgg gaataataat atcccttttc
    17821 attaacctgc aatagcgttt ctttaacaag ttccggggcg gagtctttta acaaatatgc
    17881 gttggctccc gactcaatca ttttaaggat tgtttgttcg tcgtcgtaaa cggacaaggc
    17941 tacaatttta acatttgtaa attccgaatg aatataacta gtggtttcaa aaccgttcat
    18001 tatcggcatc cctacatcaa cgagggcgat gttgggatgc gatggagtcg tgttaagcca
    18061 atcaattaat tgtttgccgt ttggcgcttc gtgaattacg ttaaaatgtt cgaaactgtt
    18121 gagtaataat ttaagtcctt ttctcaccag ctcgtgatcg tcggcaattg cgagtgtaat
    18181 gggattcatc ctgacatgta ataatactat taaacattag gacacgacat cccacacaac
    18241 ataccccatc
//
LOCUS       DAORKW010000020        17628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_31_length_17628_cov_5.594605, whole genome shotgun sequence.
ACCESSION   DAORKW010000020 DAORKW010000000
VERSION     DAORKW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17628
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31_length_17628_cov_5.594605"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PLB46_01765"
     CDS             complement(<1..610)
                     /locus_tag="PLB46_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75266.1"
                     /translation="MKRNLLLKSNIKFCLTVFSLYMPIFLFSQAPDIEWQNSIGGINA
                     DLLYSIAPTSDGGYILGGQSESGISGDKTEASLGYSDFWVIKLNSSGGIEWQNAIGGS
                     AYETIYSISQTSDGGYILGGWSSSGISGDKTEANLGTNDYWAIKLNSTGGIEWQNTIG
                     GSGIDRLYSIAQTTDGGYILGGTSDSGISGDKTEASLGYSDFW"
     gene            1403..2884
                     /locus_tag="PLB46_01770"
     CDS             1403..2884
                     /locus_tag="PLB46_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRF75267.1"
                     /translation="MEKILPNHLSELIFSSSNPTISKQISKLEREGKIRRIAARIYTS
                     NLEENAESIIKRNLLTILGKLYPGSMLSHRSAFEYKPTSAGHIFLTYTYTKKISLPGI
                     TLHFMKGLPPIAGDNPITGELYASQKERAFLENLQVTRRQGPESKTLEITEIEEKLDT
                     VIKVNGEDGLNQLRDKARIIAEQLNMQPEFIKLNKLISAFLATHTPSILSSPLAKARA
                     FGAPFDSVRIELFEKLFIELQQHEYKLYPEKNNSAQSFRNFAFFESYFSNYIEGTEFE
                     LEDAKKIIETNIPMHARDEDSHDVLGTYKLVSNKMEMKQTPEHAEQLLETLKYRHAVL
                     LSARKDKNPGQFKEKNNRAGNTHFVDVKLMRGTLIKGFDFYKALSQPFAKAAYIMFLI
                     SEVHPFVDGNGRIARIMMNAELVKEDQSKIIIPTVYREDYLDALRRLSRQQDPALYIK
                     MLQKAHQFSANIYDDNMDEMQKYLVSCHAFQEPTEAKFIIAGR"
     gene            complement(2948..4339)
                     /locus_tag="PLB46_01775"
     CDS             complement(2948..4339)
                     /locus_tag="PLB46_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF75268.1"
                     /translation="MYKCILIGLACLSMIANAQTALDIYITDQMENKHIPGVSAVIIK
                     DEKLAWVGNYGVANLSTGQAVDTNTLFMLASISKTITATAIMQLYEDGYFELTDDINL
                     HLPFEIYHPSYPDSTITMLQLLTHSAAMRDNWNILDTVYVAGDSPLPLGDFMQGYFEV
                     GGPYYYAEDNFYTYAPGADYNYCNEGIALLGYMVEVFTGQPFNSYCNEHIIEPLCMTN
                     TGWFLSELDTNLIAHPYDYISGSYYDYGLYGYPDYPDGQLRTTSLSLAKFLWMNMYNG
                     NFNGVQLLQPETIELIRASQIPFIDPTQGLVWYSYEDGGTWWGHSGGDSGVSTDMYFN
                     EDTQTGIIVLTNSDNSHTQIWNELVDFAATLNTENSPAITCNSELPLNVITSNQTHLT
                     LFPNPASSYLYIETTENFDNYAIISAEGKLQLSGKLFSNKIELNNLSSGLYYLKIRNL
                     QGEIIGVSSFVKL"
     gene            4565..5764
                     /gene="mpgS"
                     /locus_tag="PLB46_01780"
     CDS             4565..5764
                     /gene="mpgS"
                     /locus_tag="PLB46_01780"
                     /EC_number="2.4.1.217"
                     /inference="COORDINATES: protein motif:HMM:TIGR02460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050504 - mannosyl-3-phosphoglycerate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate synthase"
                     /protein_id="HRF75269.1"
                     /translation="MRIELSNAFERLGNVLIYGTQKVYALDAGPEGPNHPNDKYFVVR
                     KVANEQSWNVEQEMVIIVPIKNEKLKLLEGVITGIPHNCLIIVVSNSDRDDVDRFNME
                     KNVVENICHFSKRDFLIVHQKDPEIAELFESMGYADILGEDGLIRDGKSEGMLIGILL
                     TQLLQKKYIGFIDSDNYFPGSIYEYVRIYSSIFSLSKSDYTMGRILWHSKPKAINSNI
                     LFSKWGRVSRLTNYYLNRLISLYTGFETDIIKTANAGEHCISMNLAMSMDFGAGFTVE
                     PYNYINLFEKFGGVDKSNIQTNNNNKVVHIHQVESRNPHLHESKGNEHVNDMIDNSLG
                     CIFHSIVCPLELKKEIIKELKKAKITLQNEMPVKRAVYKALGLFDLNKAKQKFDPEKL
                     GHILTPV"
     gene            5766..6569
                     /locus_tag="PLB46_01785"
     CDS             5766..6569
                     /locus_tag="PLB46_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRF75270.1"
                     /translation="MKLLFSDIDGTITNKFSHAFEGSEIYLDKLIKGGFTVVLTSSKT
                     FEEITFIQKQLEFSEMFIFENGSGIAFPIEKYAFLDGIKSSFREQQFLIYPLAPMNLP
                     IQNWRQIMKSFFNNAPLLSGSNVEELMTITGLTKAALKRAQNRKFTETCLIEYNNSTN
                     LQIINNQLLNFQGVAEIGSRFLTVSHHGISKGIAIKKVIELLSENNRQLNTFSIGDGL
                     NDFTMFNETDNCFFLSDAVNLPDKISHCSLINPAGPAGFAKAVELLLLQ"
     gene            complement(6748..7191)
                     /locus_tag="PLB46_01790"
     CDS             complement(6748..7191)
                     /locus_tag="PLB46_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75271.1"
                     /translation="MEATQKIRFYKKLIVILTISTIIPTAMLCMSLTPFAPANNEPQS
                     VSKDVARENIRKYRESPEATETAVTGIRIEASQLESMNNIRNTNPNTSAYRMYFAQDS
                     VGAPLSIVVGIDDDGQDNTETIYSTARKGSNLCPPMCDATSPLTN"
     gene            7283..8263
                     /locus_tag="PLB46_01795"
     CDS             7283..8263
                     /locus_tag="PLB46_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75272.1"
                     /translation="MVKNFIGQGILTLLIILQISSFASAESAHHTSSSANHFSLPPID
                     KSELEEQLSHTKDEFQQLELLSQLATFYIEWEADATAADSLLTHGIELAEITYNNSLL
                     LNAYANYLIVIDDYTYSNKVEQIINKLTDLAPQINELHDVWKCKYALAKGYQLIFNND
                     KAKDYAYQALTSAIQINDIKNIIATHLVLGDVQQKMNNNVEAIRNYLDALTIAEAQED
                     VELRMDCYDRLSNFYNLIKAYDKSIQYKLKELNLAETESSPDSIRIMTLKFDLEVIAF
                     NNRTVNEKQLYKILEFADKNHVEKLKKFGMVAFRNHLIKQNDLAQLYNLF"
     gene            8283..9119
                     /locus_tag="PLB46_01800"
     CDS             8283..9119
                     /locus_tag="PLB46_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75273.1"
                     /translation="MQRIKAEDTTMYYRLNALFNEHQGNIDSAQIYFGWAATRINAIN
                     DKLRKSSFYMRYGDFFARNNLKQEAIEQYRSSYVLASSLPYYEFMLEASAKLENIYVE
                     LKDYENAYRYGSINGNISDSLSNLLKKEELQLLEIENEEVLREQRAIKEKEETKRRNN
                     IQYTAITILIATAFLLLLILGGFNVHPSLIRMVGFICFIFFFEFLILLFDTWIHHLTH
                     GEPWKILAIKILLMIGLVPLHHFIEKKVTHYLISNKINWKPNKLFTFKKAAQSTVVDE
                     TE"
     gene            9306..10355
                     /locus_tag="PLB46_01805"
     CDS             9306..10355
                     /locus_tag="PLB46_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRF75274.1"
                     /translation="MTKIHLITDQDILDYIRQYPNLSSKDIHAGLGEVASFATIKRKL
                     GSLLTKHQISATGNGKATRYFITPGSTLLAPIDVDAYFQKEIDDRKIFAEFNFALIPQ
                     LQMRNNLFFDTEIEMLESLQKKFEKNIAGLSSNEYNKEFERLAIDLSWKSSQIEGNTY
                     SLLETERLLKEQETAIGKKKDEATMLLNHKAALDFILEHTNYIQPLSLQSIEDIHSML
                     IENLGVQRNIRNRRVGISGTNYRPLDNEFQLRDVLVEMTNLINLKTNVFEKALLALSL
                     ISYIQPFADGNKRTARIISNAILMYHRYCPISFRTVDAIEYKKAMLVFYEQNNITAIK
                     KIFIEQYEFAVKTYF"
     gene            complement(10356..10955)
                     /locus_tag="PLB46_01810"
     CDS             complement(10356..10955)
                     /locus_tag="PLB46_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75275.1"
                     /translation="MQFKTSLLVFVLLLTFQVSAQFISLRGTNSSQILSDNYYNADLS
                     HAVGGEIYFSKPIGFIPLPMNYNIGLDYKNIDGINEAYLITGLTYVTVTPSGFMFATN
                     AESVTMSTYKWLNYFDLNMYNGVIIGDSTYTYKMGGEIGYNFGYVLSKTLFLHFGLGA
                     RYNYTPDYKNELYANATSLDFMVKAGILYKFKRKFNKPK"
     gene            complement(10979..11599)
                     /locus_tag="PLB46_01815"
     CDS             complement(10979..11599)
                     /locus_tag="PLB46_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRF75276.1"
                     /translation="MKTCTHCHKDNPDDFNYCRYCGADLIYVPVNKVKGFWKKIPAWA
                     WIIIIVAAVAGILFALIGSFIAVATIEGVASLVLLVLGVIGFGVAPLRKPENPGSFTR
                     AIGLSFFALMGATVDQPGNIIYNQPVEICFCDEGTSLNRDENVSNPMPGTTIIQQDFT
                     CFDEMGNPVKSINMFAVLGIRFLEYILLGYLLIAIRSLIWKLRNNT"
     gene            11710..12057
                     /locus_tag="PLB46_01820"
     CDS             11710..12057
                     /locus_tag="PLB46_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75277.1"
                     /translation="MVKVLPLQAMEQLEQNEPQEREYQPNMFWEGFFYLSFFIFANIV
                     LIPYTPKSWSFGRFYPIAMITYGLAGLFTGMIMFRLLKNAPNWLKFAIVTVLYAIILY
                     YLVDNIERFMIPD"
     gene            12244..13530
                     /locus_tag="PLB46_01825"
     CDS             12244..13530
                     /locus_tag="PLB46_01825"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRF75278.1"
                     /translation="MADKAFIKLGEKEVELPIYVGTEGEESIDINKLRDLTGFVTLDS
                     GYKNTGATKSGITFLDGELGILRYRGYPIEQLAEKSNFMEVAYLLLYGELPTNDQFVD
                     FTDRITHHTLVHEDVKQFFDGFPSNAHPMGQLCSLMCTLSSFYPDSLNPNMSKEATDL
                     TIIRILAKMATVVSWIHKKSVGHPIMYPQNKYDYVSNFLYMTFGMRTEEYVPDPVVVD
                     ALDKLLILHADHEQNCSTSTVRMVGSSQANAYASVAAGVAALWGPLHGGANQQVIEML
                     EIIKADGGNVAKFVEKAKDKNDNFRLMGFGHRVYKNFDPRAKIIKEACDNVLNKLGVF
                     DPVLDIAKQLEEVALKDPYFVERKLYPNVDFYSGIIYRAIGFPTEMFTVLFALGRLPG
                     WLSQWKEMREANEPIGRPRQIYTGPTVRDYTNIAIR"
     gene            complement(13676..14122)
                     /locus_tag="PLB46_01830"
     CDS             complement(13676..14122)
                     /locus_tag="PLB46_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75279.1"
                     /translation="MQLKSISILLLSVSILIGCGRNTNSSANEEKTSTEEIEITQPVI
                     KDETLITPEEPFKNQPNEFCTVIRKLDASYYAQGQAIEDVNAFPKKYCLLDVCLEIVN
                     PNGMIINLGDDDEVVTTSFELIKVFETEAEAKSYVEQYKIVDFKWE"
     gene            complement(14125..14619)
                     /locus_tag="PLB46_01835"
     CDS             complement(14125..14619)
                     /locus_tag="PLB46_01835"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HRF75280.1"
                     /translation="MTLLPKQEQFRQRVTSKWLFKFFLLQKMPIALLAGVRVRALNAE
                     SAVVSIPYKWLSQNPFKSVYFATQAMAAEMSTGVIGLMAIQGRAKPVSMLVTNLEAKY
                     SKKASERIFFTCENGNEIFNAVEKTLTDGEGVTVKCISTGRTKDGTIVSTFTVVWSFK
                     AKNK"
     gene            14800..15078
                     /locus_tag="PLB46_01840"
     CDS             14800..15078
                     /locus_tag="PLB46_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HRF75281.1"
                     /translation="MSAIQTDERIEVDELIDDVIGVRLIVHNDDVNTFEWVIESLVEI
                     CKHTLEQAEQCAYIIHFKGKYAVQQGTRNILAPMREQLTDRGINATIE"
     gene            15085..15768
                     /gene="aat"
                     /locus_tag="PLB46_01845"
     CDS             15085..15768
                     /gene="aat"
                     /locus_tag="PLB46_01845"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRF75282.1"
                     /translation="MPVWQLNDQLIFPNPQFAEPDGMLAIGTDFSIDRILLAYRSGIF
                     PWFCHENEFYWFSPDPRCILFPDDIVISKSMQQLIKKNKYQIKVDTNFSAVMLACAVV
                     ERKHDNETWIDEKFIRTYTELHKRGIAHSIEVYEEDELVGGLYGLSIGASFVGESMFS
                     KKSNTSKLALIYLAQQLKQFNYHFIDCQVYNEHLGSMGAKKIPRDVYLVMLTKALENP
                     PDKCFASLV"
     gene            complement(15774..16418)
                     /gene="pdxH"
                     /locus_tag="PLB46_01850"
     CDS             complement(15774..16418)
                     /gene="pdxH"
                     /locus_tag="PLB46_01850"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRF75283.1"
                     /translation="MEPIEGNLHNMRENYESGTLDIHGVYPSAMQQFAFWFEVAGKAG
                     IEEPNAMVLSTVDASNKPSSRVVLLKEFDASGFIFFTNYNSQKGKDLAHNPNACLNFW
                     WSKLERQVRINGTIEKIAEKESDDYFYSRPIGSQAGAIISPQSSVIESRAWLEQRLIE
                     VQLKGELKRPAHWGGYKLTATVIEFWQGRSNRLHDRLLYTKNENMQWEIQRLAP"
     gene            16507..17109
                     /locus_tag="PLB46_01855"
     CDS             16507..17109
                     /locus_tag="PLB46_01855"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF75284.1"
                     /translation="MSAHKGISITLLILLITIGFQTVSLAQNEEIIQLSGIVTDAQTK
                     FPVPYATVVVPSDYRGVNASEDGFFSIVVKRSDTLQFSAIGYKPKLFIVPDSGEDNIE
                     SIAVKLQSDTLSLDPFVIYPWPSKEDFRESFLAYKAIQEYTMGPIPGIKRPDQIDTVP
                     QAPTLIKNPISFIYEEVVKPIQWSKKKKHMVDELPVWKEE"
     gene            17326..>17628
                     /locus_tag="PLB46_01860"
     CDS             17326..>17628
                     /locus_tag="PLB46_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HRF75285.1"
                     /translation="MKQRNILIAGAGSAGTMMANHLKRKLNHHEWKITIVDEATTHYY
                     QPGFLFLPFGIYDEHEIQKPITKYIPDDVDFINTAIDKIEPERNVLTLINGAELPYD"
BASE COUNT         5852 a         3119 c         3078 g         5579 t
ORIGIN      
        1 cccaaaagtc ggaatatccg aggctcgctt ctgttttatc ccccgaaata ccggagtcgg
       61 aagtacctcc taaaatatac ccgccatcag tagtttgagc gatactatac aatctatcaa
      121 ttccacttcc gccaattgta ttctgccatt caatgccacc agttgaattc agttttattg
      181 cccaatagtc gttagttcca agattcgcct ctgttttatc tccggaaata ccagagctag
      241 accaacctcc taaaatatac cccccatcac ttgtttggga gatactatat attgtttcat
      301 atgcactgcc tccaattgca ttctgccact caataccacc ggatgagttc agctttatca
      361 cccaaaagtc ggaatatccg aggctcgctt ctgttttatc ccccgaaata ccggattcgg
      421 attgtccacc taaaatatat ccaccatcac tagttggggc tatactatac aaaagatctg
      481 cgttaatacc tccaatcgaa ttctgccatt cgatatctgg tgcctgagaa aataagaaaa
      541 taggcatgta taacgaaaat acagttaagc aaaatttaat attcgattta aggagcaaat
      601 ttcttttcat aacagtagga attaacttta gatgtgttaa ttgttttaca aattttgcct
      661 aaggaataaa gttggattta taaatttaat aaaaaaaccc ttaacatagt tttcgaattt
      721 atactgtgtt ttcgtggagg atagatttag taaaattttg agaggttagt aaattttacc
      781 ttacccgttt gaaacctttt aataccagca ccttagcgcc tagcgtattt cagctcatgc
      841 aggctgctct gtaagcttag tggttgtgag cttctgacgt aaacgagcaa gacagatgtt
      901 ttcacacaat tgcttaaaag tatgctttgg cgttggaagt aatggagctg catttcgtgg
      961 tataattgga caatttgcca actattttaa cctaaaagat ctgtttttgg ttaaaatagt
     1021 tagcatttta ggcaaaattt tatgtaacta tttgtaatta ttatgcgcaa ttaatctatt
     1081 aaatggtcaa aataattacc atttaattaa cttttataag cattgaatat caccgataat
     1141 ataaacagat gcaaatatca atctaagttc ttgtgtgggt atcgtctgat tgttgatttt
     1201 ataatggaaa atatttgcca aaatcggctt ttgttggaaa atatttgccg ttacaattgt
     1261 tagcattaag gtttaatgga aaatatttgc aaaatatgat ctatttctcc cagctaaaac
     1321 tagctctata ctggaaaata tttgcattaa atggcaatat aatggaaaaa ttttgccaaa
     1381 tttgtgtaat aatagcgtta taatggaaaa aattttgcca aatcacctat cggaactaat
     1441 cttttcttca tctaatccaa ccatttcaaa gcaaatttcc aaattggagc gggaaggtaa
     1501 gataaggagg attgcggctc gtatttacac ctcaaatctt gaagaaaacg ccgaatccat
     1561 tattaagaga aatttgctca caattctagg caaattatat cccggctcca tgttgagtca
     1621 tcggtcagca tttgagtata agccaacctc tgccgggcat atatttctca cctatactta
     1681 tactaagaaa atatcattac ctggcatcac gctccatttt atgaaaggtt tgccacctat
     1741 tgctggtgac aatccaatta caggcgagtt gtacgcttct caaaaggaac gagccttcct
     1801 tgaaaattta caagtaacca gaagacaagg accagagtcc aaaacacttg aaataactga
     1861 aattgaagaa aagttagata cagttataaa agtaaatggt gaagacgggc tgaatcaact
     1921 gcgcgataaa gcacgaatca ttgctgagca actgaacatg caaccggaat ttataaagtt
     1981 aaataaattg attagtgcat ttctagcaac acatactcct tcaatacttt catcaccttt
     2041 agcgaaagca agagcttttg gcgccccttt cgacagtgtg agaattgaat tatttgaaaa
     2101 attatttatt gaattgcagc aacacgaata taaactgtat cctgaaaaaa ataactcagc
     2161 tcagtcattt agaaattttg cattcttcga aagctatttt tcaaattata ttgaaggaac
     2221 tgaatttgaa ttggaagatg caaaaaagat tattgaaacg aatataccta tgcacgcaag
     2281 ggatgaagat tcacatgatg tgcttggtac ctataaatta gtttccaaca aaatggaaat
     2341 gaaacaaact cctgagcatg cagagcaatt gttagaaacg ctgaaataca gacatgctgt
     2401 tttattaagt gccagaaaag ataaaaaccc tggccagttt aaggagaaaa ataatagggc
     2461 tggaaataca cattttgttg acgtaaaatt aatgagagga acattaatta aaggtttcga
     2521 tttttataaa gcattatccc aaccctttgc aaaagcagcc tatatcatgt ttttaataag
     2581 cgaagtgcat ccgtttgtag atggaaatgg aagaatagca agaataatga tgaatgcaga
     2641 attggtaaaa gaagatcaga gcaaaataat tattccaacc gtttatcgag aagattacct
     2701 tgatgcacta aggagattgt cgaggcaaca ggacccggca ttatacataa aaatgctgca
     2761 aaaggcacat cagtttagtg ctaatattta tgatgataat atggatgaaa tgcaaaaata
     2821 tttggtaagt tgccatgcat ttcaggaacc aacagaagct aagtttatta ttgcagggcg
     2881 atagaattta ttgtatgaaa cctaactttt caacaatgtt taagggaaaa aggtaaaatt
     2941 caactattta caattttaca aaactactca caccaataat ttcaccctgt agattcctga
     3001 tttttaaata atataatcct gatgaaaggt tgtttaattc aatcttattt gaaaataatt
     3061 tcccacttaa ctgcaatttt ccttctgcag aaataattgc ataattatca aaattttctg
     3121 ttgtttcaat atacaaataa ctacttgccg gattcggaaa aagagttaaa tgagtttgat
     3181 tggaagtaat tacatttagt ggaagttcac tgttacaggt tattgcagga ctattttcag
     3241 tattcaacgt ggcagcaaaa tcaactaatt cattccatat ttgtgtgtga ctattgtcgc
     3301 tattagttaa aacgataatt ccggtttgtg tgtcttcatt aaaatacata tcggttgaaa
     3361 cacctgaatc accgccacta tgtccccacc aagtgccgcc atcttcataa ctataccaaa
     3421 ctaatccctg tgttggatct ataaacggaa tttgtgatgc acgaattaat tcaatcgttt
     3481 caggttgtaa taattgaaca ccattaaagt tcccgttata catattcatc cataaaaatt
     3541 ttgcaagcga taatgatgtg gtgcgtaatt gaccatcagg ataatcagga taaccatata
     3601 aaccataatc ataataactg ccagaaatat agtcataagg atgtgcaatt aaattggtat
     3661 ctaattcact taaaaaccaa cctgtatttg tcatacacaa tggttcgatg atatgttcat
     3721 tgcaataact attaaacggt tgcccggtaa atacttctac catatatcct aataacgcaa
     3781 taccttcatt acaataatta tagtccgcac cgggcgcata agtgtaaaaa ttatcttccg
     3841 cgtaataata aggtccgccg acttcaaaat aaccttgcat aaaatctcct aatggcaggg
     3901 gtgaatcacc tgcaacatac accgtatcca atatattcca attatcgcgc atggctgcag
     3961 aatgtgtcaa aagttgcagc atagtaatgg ttgaatctgg gtaggaggga tggtaaattt
     4021 caaatggcaa atggaggttg atgtcatcag tcaattcaaa ataaccatct tcatataatt
     4081 gcatgattgc ggtagctgta atcgtttttg aaatagatgc cagcatgaat aaggtattgg
     4141 tgtcaaccgc ctgacctgtg cttaaatttg ccacaccata gttgccaacc caggctagtt
     4201 tttcatcctt gattattaca gcactcacac caggaatatg tttattttcc atttggtcgg
     4261 taatgtaaat atcaagggct gtttgtgcgt ttgcaatcat actcagacat gccagaccta
     4321 taagtataca tttatacatg tgttaaaact aaataaaatt tgtgaaatta taatatgcgt
     4381 ggatttttta ctaaatcaat tggttttatt ttaatattaa ataaaaataa atgttaaaga
     4441 atataacaat tttcatttaa atttatttac tttaattgat tataattcat gtatacttga
     4501 caatgtaaac tattttttat atatttgctt atattgattt ttttatcatc taaattttaa
     4561 tgttgtgaga atagaattat caaatgcttt tgagcgatta ggtaatgtat taatttacgg
     4621 cacgcaaaaa gtgtatgcat tagatgcagg tccagaaggc cctaaccatc caaatgataa
     4681 atattttgtt gtacgtaaag tggctaacga acagtcgtgg aatgttgaac aggaaatggt
     4741 aattatcgtg ccaattaaaa atgaaaaatt aaaattgctg gaaggtgtta ttaccggaat
     4801 tccacataat tgtttgatta ttgtagtttc caatagtgat agagatgatg tagacagatt
     4861 taacatggag aaaaatgtcg tggaaaatat ttgtcatttt tctaaacgtg atttccttat
     4921 cgttcatcaa aaagaccctg aaattgctga gttatttgaa agtatgggtt atgctgacat
     4981 tttaggtgag gatggattaa tccgcgatgg aaaatctgag ggaatgctta ttggcatttt
     5041 gctcacacaa ttactacaaa aaaaatatat cggttttatt gattcagata attatttccc
     5101 tgggtcgatt tatgagtatg tacgcattta ttcgtccata ttctcactgt ctaaatcaga
     5161 ttacacgatg ggtagaattt tgtggcatag caaacccaag gctattaatt ctaatatact
     5221 gttcagcaaa tggggacgtg taagcagatt aacaaattat tatctcaata gactcattag
     5281 tttgtatacc ggttttgaaa ccgatattat taaaactgct aatgcaggcg aacattgcat
     5341 aagtatgaat ttagcaatga gtatggactt tggtgcaggt tttaccgttg aaccatataa
     5401 ttatatcaat ttgtttgaaa aatttggtgg tgttgataaa tcgaacattc aaacaaataa
     5461 taataacaaa gtagttcata tacatcaggt ggaatccaga aatccgcatc tacatgaatc
     5521 aaaaggaaat gaacatgtaa acgatatgat tgataattct cttggttgta tatttcattc
     5581 aatagtttgt ccgttggaat taaaaaaaga aataattaaa gaactcaaaa aggcaaaaat
     5641 tactttacaa aatgaaatgc cggtaaaaag ggctgtctac aaagcattag gactttttga
     5701 tttgaataaa gccaaacaaa aatttgaccc tgaaaaattg gggcatattc taactcctgt
     5761 ttaacatgaa actcctgttt agtgatatcg atgggactat aacgaacaaa ttttcgcacg
     5821 cttttgaagg ttcggaaatt tacttggata aacttataaa gggtggcttt acagtagtgc
     5881 tcacttcctc taaaaccttc gaggagataa ctttcattca aaaacaactc gaattttcag
     5941 agatgtttat attcgaaaat ggaagtggaa ttgcattccc catagaaaaa tacgcttttc
     6001 ttgatggtat aaaatcgagt ttcagagaac aacaattttt gatttatccg ttagcgccaa
     6061 tgaatttgcc aattcaaaac tggaggcaaa ttatgaagtc attttttaat aatgctccat
     6121 tgttatccgg ttccaatgtt gaagaattaa tgactataac aggcttaaca aaggcagcct
     6181 taaagcgcgc ccaaaatcga aaattcacgg aaacatgttt aattgagtat aacaattcaa
     6241 caaatttgca aattattaat aaccagttat tgaatttcca aggtgtagct gaaattggct
     6301 cacgcttttt gacggtttca catcacggga tatctaaagg tattgcaatt aaaaaagtta
     6361 ttgagttatt aagtgaaaat aacaggcaat taaatacctt ttcgattggg gatggtttaa
     6421 acgatttcac tatgtttaac gaaactgaca actgcttctt tttgtcagat gcagtgaatt
     6481 tacccgataa aatttcacac tgtagtttaa ttaacccggc cggaccagca ggttttgcca
     6541 aggctgtaga acttttatta ttgcaataga ttacaattgt ttgaattgcg acttgaaaac
     6601 aataatgtta ccgcatgcaa ataattcgta aagagtttgt tggtgaaaat gcgagtaatt
     6661 tgcttaattg aaagtaagta gtaaactaaa ataaacaaag ggattttgaa ttgggacgtg
     6721 gttggcatcc caattcaaaa aaacaaatta attggtaagc gggctggttg catcgcacat
     6781 tggggggcaa agatttgacc cttttctagc ggttgaataa atagtttcag tattatcctg
     6841 tccatcatca tctataccaa caacaattga aagtggagca ccaactgaat cttgtgcaaa
     6901 atacatccga tacgctgaag tatttggatt tgtatttcta atgttattca ttgactccaa
     6961 ctgactggct tcaatgcgaa tgccggtaac tgcagtttcg gtagcctccg gactttcgcg
     7021 gtatttgcga atgttttcac gtgctacatc ttttgataca gactgaggtt cgttatttgc
     7081 aggcgcgaaa ggggttagcg acatgcacaa catggctgtg ggaataatgg tgcttattgt
     7141 aagaattaca ataagttttt tgtaaaacct aattttttga gttgcttcca taaatatgta
     7201 tgtttgtgta agttatgcgt taaagttact attctttccc ttagcattat gcataatatc
     7261 acactataca gctaaactca ttatggttaa aaattttatt ggtcagggca tcctcactct
     7321 gcttattata ttgcagattt cctcatttgc aagcgctgag tcagcacatc atacttcttc
     7381 atccgctaat cacttttcct tacctcccat tgataaatcc gaattagagg aacaattaag
     7441 tcacaccaaa gatgaattcc aacaattgga attattgagt cagctagcca ctttttatat
     7501 tgaatgggaa gcagatgcca ctgccgctga tagtttattg acacatggaa ttgaacttgc
     7561 cgaaattacc tataataaca gtttgttatt aaatgcgtat gccaattatt taattgtaat
     7621 tgacgattat acatatagta acaaggttga gcaaattatt aataaactca ccgatctggc
     7681 accacaaatt aacgaattac acgatgtttg gaaatgtaaa tacgctttgg caaaagggta
     7741 tcaattgata tttaacaacg ataaggctaa agattatgcc taccaagcct taaccagtgc
     7801 aatacaaatt aatgacatca aaaacataat tgctactcat ttggttttag gagatgtcca
     7861 gcaaaaaatg aataataatg ttgaagctat tagaaattat ttagatgcat taacaattgc
     7921 ggaagctcaa gaagatgttg aattgcgcat ggattgttat gaccgcttgt ccaattttta
     7981 taatttgata aaggcatatg ataaatctat tcaatacaaa ttaaaggaat tgaacttagc
     8041 ggaaacagaa agcagccccg attcaatccg aataatgaca ttaaaattcg acctggaagt
     8101 tattgcattt aacaacagaa cagtaaacga aaaacaactg tataaaattc ttgaatttgc
     8161 cgacaagaac catgtcgaaa aattgaaaaa gtttgggatg gtcgcatttc gaaaccacct
     8221 gattaaacaa aacgatttag cgcagttata taatttattt taataatgaa ttcccggctg
     8281 aattgcagcg cattaaagct gaagatacta ctatgtatta caggcttaat gcgttgttta
     8341 acgaacacca gggcaatatc gactcagcac agatttattt tggttgggca gctaccagaa
     8401 taaatgcaat aaacgataaa ctgaggaaat caagttttta tatgcggtat ggtgattttt
     8461 ttgcacgtaa taatctcaaa caagaagcaa ttgaacaata tcgaagttca tatgtgttgg
     8521 catcatcatt accttattac gaatttatgc tagaggcatc agctaaattg gaaaatatat
     8581 atgttgaatt aaaagactac gaaaacgcct atcgttatgg cagtataaat ggaaatattt
     8641 ctgacagcct gagcaatttg cttaaaaaag aagaattaca attactcgaa attgaaaatg
     8701 aggaagtttt gcgtgagcaa cgtgccatta aagaaaagga agaaacaaaa agaagaaata
     8761 acatccaata taccgcaatt actatcctga ttgctactgc atttttatta ctactgattt
     8821 taggcggatt taatgtccat ccttctttaa tcagaatggt tgggtttatc tgttttattt
     8881 tcttctttga atttttaatc ctactgtttg atacttggat tcatcacctt actcatggcg
     8941 agccatggaa aattctcgcc ataaaaatat tgctgatgat aggtttggta cctttacacc
     9001 actttattga gaaaaaagta acgcattact taataagtaa taaaattaat tggaagccga
     9061 ataaattatt caccttcaaa aaggctgctc aatccactgt tgtggatgaa acggaatgat
     9121 actactcaag gtaaacacca cattttttgt tttttagctg attgagttgg aaagttatca
     9181 aactgagctc taaagtaata aatcatgagc tttatcggct ttaagctcaa attatttaaa
     9241 atttgggctc aaaatttgca tgtttgagcc ccaaatagta attttatgag ctcaaaaggg
     9301 gtaagatgac caaaattcac ttaataacag accaggatat actagactat atcaggcaat
     9361 atcccaattt atcttctaaa gatattcatg ccggtttagg cgaagtggca tccttcgcaa
     9421 caataaaacg taaacttggt agtttattga ccaaacacca aatttcagca acaggtaacg
     9481 gaaaagctac caggtacttc attactcccg gttccacttt attggctcct atagatgttg
     9541 atgcttattt tcagaaagaa attgatgata ggaaaatatt tgcagaattt aattttgcat
     9601 taataccaca attgcaaatg cgcaacaatt tattctttga tactgagatt gaaatgttgg
     9661 aatcacttca gaaaaaattt gaaaaaaata ttgcgggttt gtcttcgaat gaatataaca
     9721 aagaatttga acgattagca attgatttga gttggaaatc atcgcaaatt gaaggtaaca
     9781 cctacagttt attggaaact gaacgattac tcaaagaaca ggaaaccgcg attggtaaga
     9841 aaaaagacga ggcaacgatg ttattaaatc acaaagctgc tttagatttc attttagagc
     9901 atacaaatta tattcaaccg ctaagtttac aaagtatcga agatatacat agtatgttga
     9961 ttgaaaatct cggcgttcag cgcaatatcc ggaataggag agttggtatt tcaggaacaa
    10021 attatcgtcc tttggataat gaatttcaat taagagatgt tttagttgaa atgacaaatc
    10081 ttattaattt aaagacaaat gtatttgaaa aagctttatt ggctctctct ttaatatctt
    10141 atattcaacc atttgctgac ggtaataagc gcactgctag gattattagt aatgctatcc
    10201 taatgtacca tcgctactgc ccaatttcat tccgcactgt tgatgctatt gaatacaaaa
    10261 aagcgatgct tgttttttat gaacaaaata atattactgc cataaaaaaa atatttatcg
    10321 aacaatatga atttgcagta aaaacttatt tttaattact ttggtttgtt aaacttgcgt
    10381 ttaaatttat acaatattcc cgcttttacc ataaaatcca atgatgtagc attggcatat
    10441 aattcatttt tgtaatcagg cgtgtagtta tatctggcac ctaatccaaa atgaaggaat
    10501 aaggttttac tcaatacata accaaaattg taaccaattt ctccacccat tttataggta
    10561 taagtagaat cgccaataat tactccatta tacatattca ggtcaaaata atttaaccat
    10621 ttataggtcg acatggttac gctttcggca ttagtggcaa acataaaacc acttggtgta
    10681 accgttacat aagttaagcc tgttattaaa tacgcttcat ttataccatc tatattttta
    10741 tagtccaaac caatattgta attcattggc agtggtataa atcctatagg ttttgaaaaa
    10801 taaatttccc ctccaacagc atgcgataag tcggcattat aataattgtc ggataaaatt
    10861 tgtgagctgt ttgttccgcg caagctgata aattgagcag aaacctggaa agttaatagc
    10921 aacacgaaaa cgaggagtga tgttttaaat tgcataggaa ctgaacgaat tttgatgttt
    10981 aagtattgtt tcgcaatttc caaataagac tgcgtattgc aatgagtaaa taaccaagca
    11041 aaatgtattc taaaaaccga atacctaata ctgcaaacat gttaatggat tttacaggat
    11101 tgcccatttc atcaaaacag gtaaaatcct gttgtatgat agtagtaccc ggcatcggat
    11161 tactgacatt ttcatctctg tttaaagaag tgccctcatc gcaaaaacat atttcaacag
    11221 gttgattgta aatgatgttg ccgggttggt cgactgttgc gcccataagc gcgaagaagc
    11281 tgagccctat agccctggta aaacttcccg ggttttcagg tttacgcagc ggtgccacgc
    11341 caaatccgat aacacctagc actaataaaa ctaaactggc aactccttca atagttgcta
    11401 ctgcaataaa tgaaccgata agggcaaata aaatgcctgc aacagcagca acaattatga
    11461 taatccatgc ccaagccgga atttttttcc aaaagccttt tactttattt accggaacgt
    11521 aaatgaggtc agctccgcaa tatctgcaat agttaaaatc atcagggttg tccttatggc
    11581 aatgtgtaca ggttttcatc gctgccaaat tacagttttt tgaattgagt ataaactccc
    11641 ttaagttgcc caaatagcaa ttatagcagc ttttgaatgt tgtatttttg ttaaataaag
    11701 atgctgtttt tggtaaaagt attacctttg caggcaatgg agcagttaga acaaaacgaa
    11761 cctcaagagc gggaatatca gccaaatatg ttttgggaag gtttttttta tctttccttt
    11821 ttcatattcg cgaatattgt cctaataccc tacacaccta aatcgtggtc gtttggcagg
    11881 ttttatccaa ttgcaatgat tacctacggt ttagcaggtt tgtttaccgg gatgatcatg
    11941 ttccgattac tcaaaaatgc acctaactgg ctcaaatttg ccattgtaac ggtgctttat
    12001 gccatcattt tgtattatct cgtggataat atcgaacggt ttatgatacc cgattaattt
    12061 atcatcctcc agaacctcca ttttccgcct gttttttgca atatcagtaa actgaaggtc
    12121 atgatattat ctttttgtaa aaaatgttag gttttatgac ctgataatta ttaatgtgtc
    12181 tttccttttt ttacctttgt ctacaatatt tattaatcca aaaaggacaa tttgaccaaa
    12241 attatggctg ataaagcgtt cattaaactc ggtgagaagg aagtagaatt acctatttat
    12301 gttggcaccg aaggagaaga gtcgatagac attaataaac tccgtgattt aaccggattc
    12361 gttaccctcg atagtggtta caaaaacacg ggcgcaacta aaagtggcat tacattcctg
    12421 gatggtgaac tcggtatatt acgttaccgc ggttacccaa tcgagcaatt ggccgaaaaa
    12481 tcaaatttca tggaggtggc ttacctcttg ttatatggtg aactgccaac caatgaccaa
    12541 tttgttgatt ttacagacag aattacccat catacgctcg tacatgagga tgtgaaacaa
    12601 ttttttgatg gttttccgtc aaatgcacat ccaatgggtc aattatgctc actaatgtgc
    12661 accttgagct cattttatcc ggattcctta aatccaaaca tgagcaaaga agctacagat
    12721 ttaactatta ttcgcatttt ggcgaaaatg gcgactgttg tttcatggat acataaaaaa
    12781 tcagttggac atccaatcat gtatccgcaa aacaaatacg attatgtaag caacttctta
    12841 tacatgactt tcggtatgcg tactgaagaa tatgttccgg acccggttgt tgtggatgct
    12901 ttagataaat tattaatctt acatgccgac cacgaacaaa actgttcaac atcaacagta
    12961 cgtatggtgg gttcaagtca ggcaaatgct tatgccagcg ttgcagcagg tgttgccgct
    13021 ttatggggac cactgcatgg tggtgctaac caacaggtta ttgaaatgtt ggaaataatt
    13081 aaagccgacg gtggtaatgt tgccaaattt gtggagaaag caaaagataa aaatgacaat
    13141 ttccgtttaa tgggatttgg tcaccgcgtg tataaaaatt tcgacccacg cgctaaaatt
    13201 ataaaagaag cttgcgataa tgttttaaat aaattaggtg tatttgatcc tgttctcgat
    13261 atcgctaaac aattagaaga ggttgcatta aaagaccctt attttgttga acgtaaatta
    13321 tatccgaacg tagattttta tagcggaata atttatcgtg caataggttt cccaaccgaa
    13381 atgtttaccg tattgtttgc attaggtcgc cttccgggtt ggttgtcaca gtggaaagaa
    13441 atgcgtgaag ctaacgagcc aatcggtcgc ccgcgtcaaa tctacaccgg accaacagtt
    13501 cgtgattata ccaatattgc aataagataa ttgtaataat aaacctacta taaaaccctt
    13561 ccttgttgaa gggttttttt atgcctttaa ttaaacgaca agaaataatt attgagaaaa
    13621 atataatagt ttttaactat caaaatgcac tttttacaaa tagaacagcc taacttcact
    13681 cccatttgaa atcaacaatt ttatattgtt caacataact ttttgcttcg gcttcggttt
    13741 caaaaacttt aattaattca aatgaagtag ttaccacttc atcgtcgtcg cctaaattga
    13801 taatcatgcc atttggattt actatttcga gacatacatc taacaggcag tatttttttg
    13861 ggaaagcatt tacatcttca attgcttgcc cttgcgcata atagctggca tctaatttcc
    13921 tgataaccgt gcaaaattca tttggttgat ttttaaatgg ctcttccggt gtaattaatg
    13981 tttcatcttt tataacaggt tgcgttattt caatttcttc ggttgatgtt ttttcttcat
    14041 tagccgatga attggtgttg cgaccacaac caattaaaat acttacactc aacaataata
    14101 tggaaataga tttcaattgc atgtttattt attttttgct ttaaacgacc aaacaacggt
    14161 aaaggtactt acaattgttc catcttttgt tcgtccggtt gatatacatt ttacggtaac
    14221 accttcgcca tcagttaacg ttttttctac agcattaaat atttcattgc cgttttcaca
    14281 ggtaaaaaat atgcgttcac tggctttttt tgaatatttt gcttccagat ttgtcactaa
    14341 catcgaaacc ggttttgctc gaccttgaat cgccattaaa ccgataactc ccgtgctcat
    14401 ttctgccgcc atagcctgag tggcaaaata aaccgacttg aatgggttct gactaagcca
    14461 tttataagga atactcacta cagcactttc tgcattcaaa gcccgcacac gaactcctgc
    14521 cagcaaagca atgggcatct tttggagtaa aaaaaacttg aataaccatt tactagttac
    14581 ccgttgtctg aattgttctt gttttggaag taaagtcata atcaggcccc gaatttaagg
    14641 caaatagata aaacattgtt cgaaggccgg gtttgctctg gttaagggga actgtcatcc
    14701 accccaacca ctttgaaacc cttatgcata tagggttctg acgatttttc acgaaaaatg
    14761 gcggttttgc actacctttg tgcaaaatta ttacttaaca tgagtgctat acaaacagat
    14821 gaacgtattg aagtagatga gttgatcgat gatgttatcg gcgtaagact cattgtacac
    14881 aacgacgacg tgaatacctt tgaatgggtg attgaaagtc tggttgaaat ctgcaaacac
    14941 accctcgaac aggctgaaca gtgtgcttac atcattcatt ttaagggcaa atatgccgta
    15001 caacaaggca ctagaaatat tttagctcca atgcgcgagc agcttaccga tcgtggtatt
    15061 aatgctacta tcgaataata agttatgcct gtttggcaat taaacgacca acttattttc
    15121 cccaatccgc aatttgctga gcccgatggc atgttagcaa ttggtaccga tttttctatt
    15181 gatagaattt tattggcata tcgctccggt atcttcccct ggttttgtca cgaaaatgaa
    15241 ttttattggt tttcacctga ccccagatgt attttatttc cggatgatat tgtgatatcg
    15301 aaaagtatgc aacaactaat taaaaaaaat aaatatcaga ttaaagttga taccaatttt
    15361 tctgcagtaa tgttggcatg cgctgtggtt gaaagaaaac acgataacga aacatggatt
    15421 gatgaaaaat ttattcgcac ctataccgaa ttacataaaa gaggtatagc acattccatt
    15481 gaagtatatg aggaagatga attagttggt ggattatacg gtttaagtat tggtgcaagt
    15541 tttgtcggag aaagtatgtt cagcaaaaaa agtaatacca gtaaattggc attgatttat
    15601 ttagcacaac aactcaaaca attcaattac cattttatcg attgtcaggt ttataatgaa
    15661 catcttggtt ctatgggagc aaaaaaaatt cccagagatg tctatttggt aatgctgaca
    15721 aaagcgcttg aaaacccgcc ggataagtgt ttcgcgagtt tagtataaag tatttaagga
    15781 gctagtcgct gaatttccca ttgcatattt tcatttttag tataaagtaa acggtcgtgc
    15841 aaacgattac ttctaccttg ccaaaattca ataactgttg ccgttaattt atatccaccc
    15901 caatgtgcag ggcgctttaa ttcacctttt aactgcactt caattaatcg ctgttcaagc
    15961 catgctctgc tttcaattac actgctttgt ggagaaataa ttgcgcctgc ctgactgcca
    16021 attggtcgcg aataaaaata gtcatcactt tctttttcag ctattttttc aatagttcca
    16081 tttatacgta cctgacgttc taatttgctc caccaaaaat ttaaacatgc atttggatta
    16141 tgtgctagat cttttccttt ctgactatta taattcgtaa aaaaaataaa acctgatgca
    16201 tcaaattctt ttaataacac tacacgagaa gatggtttat tacttgcatc aacggtagat
    16261 aaaaccatgg catttggttc ttcaattcct gctttacctg ctacttcaaa ccaaaatgca
    16321 aattgttgca tagccgatgg ataaactccg tgaatatcaa gagttccact ttcgtaattt
    16381 tcgcgcatat tgtgcaaatt tccttctatc ggttccatat atgttaatta aggtgaaatt
    16441 acctgcaaat cttacaaatt atttcataac taatcataac ttgcgtcaat tatcgttaaa
    16501 ggattaatgt ctgcacacaa aggaatatcg attaccctgc tgattttatt gattacaata
    16561 ggttttcaaa ctgtatcatt agctcaaaat gaagaaatta ttcagctttc ggggatagtt
    16621 accgatgctc aaactaagtt tcccgtgcct tatgctactg tagtagtgcc ctccgattat
    16681 cggggtgtaa atgcatctga agatggcttt ttttctatag tagttaaacg cagcgacaca
    16741 cttcagttta gtgccatagg ttataaacca aagctcttta ttgtgccgga ttccggggaa
    16801 gataatattg aatctatagc agtaaaacta caatccgaca ctttgtcgct cgacccattt
    16861 gtaatttatc cctggccaag taaagaagat tttcgcgaat cttttttggc ctataaagct
    16921 attcaggaat acaccatggg tcctataccg ggcataaaac gacctgatca aatcgacacc
    16981 gtcccccaag caccgaccct aattaaaaat cctatttctt tcatctatga agaagtagta
    17041 aaacccatcc aatggagtaa aaagaaaaaa cacatggtcg acgaactccc cgtctggaaa
    17101 gaagagtaac cctccgcatc tccccctttc cgcgcgaacc aaacccataa taccctttgc
    17161 gcccttaaat aaataccccc accaaagcac atacaataaa atccaccttc gggcctttgc
    17221 gagaaaaaac cataaatcgg tgacatctat cacttttttc tgtgccaatc atcacgttcg
    17281 ttgacctgca catgctagta gttttgtgat gtaaatcatc aaacaatgaa acaacgtaat
    17341 attttaatag caggtgcagg ttcagccgga accatgatgg ccaaccacct aaaaagaaaa
    17401 ctcaatcacc acgaatggaa aataaccatc gttgatgaag ccactacaca ttattatcaa
    17461 cccggatttt tgttccttcc ttttggtatt tacgacgaac atgaaatcca gaaaccaatt
    17521 accaaataca ttcctgatga tgtagatttt atcaatacag caatcgataa aattgaaccg
    17581 gaaagaaatg tattaacctt aattaatggt gccgaattgc catatgat
//
LOCUS       DAORKW010000021        17607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_32_length_17607_cov_6.227040, whole genome shotgun sequence.
ACCESSION   DAORKW010000021 DAORKW010000000
VERSION     DAORKW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17607
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32_length_17607_cov_6.227040"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..663
                     /locus_tag="PLB46_01865"
     CDS             196..663
                     /locus_tag="PLB46_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75286.1"
                     /translation="MRFKHLFTSICFVLTFSAGFTQSIDISGTWVLNLEKSQLEAKSE
                     GFTGSKFIISQKGEKFYLTRYHYYGERSNKISFKMKADGKTRPVKLLFRGKLEPAPTT
                     LKASIWKNNFSNIVDYSFGSSQNELIADEVMKSKHDNHHNIWVFDKVIIETTK"
     gene            704..1204
                     /locus_tag="PLB46_01870"
     CDS             704..1204
                     /locus_tag="PLB46_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF75287.1"
                     /translation="MVLQFRTGTISDIPQLQQLAISSWGQYEKVLSPDDWNKLFTNLS
                     NPDTYAELLDIAHCEVCTNENNTIIGMAFLVPSGNPSDIYDSSWCQLRFVSVDPNYSG
                     FGIAKTLTQKCITLAQQNKEQIMALHTSEMMDKARHIYESLGFTILKEIPPRFNKKYW
                     LYTLKL"
     gene            1482..2219
                     /locus_tag="PLB46_01875"
     CDS             1482..2219
                     /locus_tag="PLB46_01875"
                     /inference="COORDINATES: protein motif:HMM:NF039649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6090 family protein"
                     /protein_id="HRF75288.1"
                     /translation="MTKKPRFTGLNKFLLEFLSIFIGVFAAFALDNWNEDRKDHITEM
                     SILQEINNGLKQDLLDIQVNTKGHEYGIIAIQHFRNIVLNVPVNNDSITFHYFNLFRD
                     FVSVQNNSGYETLKSKGLEIISNDSLRSKIIALYEGDYNTIRKLEEEYDELQFQENYY
                     KDFNTKMSPFYQLDSLGTLKGVLSPVNLSTPDKNQILSDFWKMQNNRVFIIQYYLEVE
                     KRIKALQTEINKELHLTLGNANSCSAP"
     gene            2263..3189
                     /locus_tag="PLB46_01880"
     CDS             2263..3189
                     /locus_tag="PLB46_01880"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRF75289.1"
                     /translation="MRKNYKLIAETLIVFLLNGLLAIVLFEFISNDLLRWSLYMPLLI
                     FQGLWYYRLYIIGHECAHKKLIPENNTVNDIVGSVALIPLLVPINIYRKIHYFHHGFN
                     RKDPHTSALDTFVITGKFKKVKTVYYYFIWYLSVFFGGFFLHSLISVMLFLFVPSKLS
                     SRISPAFKGWQMKDQFVAILLFCIGLLFHFATYQLLGKQYYLLVLGFPMLSFAWVLSL
                     LVYIFHYDTTISEEVRFNVRSVRPVPVISWILMNFNEHATHHQYPNIPWYELPSKRKQ
                     LPEAYALKNQRTTNFFVAIFRQLKGPSIHYEN"
     gene            3179..3811
                     /locus_tag="PLB46_01885"
     CDS             3179..3811
                     /locus_tag="PLB46_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75290.1"
                     /translation="MKINIRYAKLEDAVAMVEIKNQLSFKNINGTTTTGGFLLGTTLQ
                     TYQEYIANDYCLVAENEYQIIGFGIILKNTTLQQSDIWTKRHAANWEIDITKYESPNV
                     CYFEQLAFLRGYTRVVIRLCYQLLQLAFNTHDNLFTTTVSSPILNLAAIPFIEKAGGR
                     KIGCINEVYPEIGLILSDIYLIEKAIYIKRLEHSGLQPLLNRIPYELQIF"
     gene            3955..4275
                     /locus_tag="PLB46_01890"
     CDS             3955..4275
                     /locus_tag="PLB46_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF75291.1"
                     /translation="MNLRRDVFQAIADPTRRGILLLVASQAMTAGAIAANFDTARPTV
                     SKHLHILTECELLTQVQDGREIYYKTNVKKMKAVADFIEPFRKMWDDRFNKLEEVMKK
                     RHKK"
     gene            4282..4746
                     /locus_tag="PLB46_01895"
     CDS             4282..4746
                     /locus_tag="PLB46_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF75292.1"
                     /translation="MELKTKIHAEDGKQELFITRNFDLPVELLFEAYTDAEIVAQWMN
                     TNVLKLENKNHGSYLFETTDPMGNKHRFNGTIHEMQTNAFITRTFQMENTAFPPQLEF
                     LVFEKITDDSSKLTMHVVYQSVAVRDQILQLPFAKGINMGHNRIQEILSKNN"
     gene            4748..5125
                     /locus_tag="PLB46_01900"
     CDS             4748..5125
                     /locus_tag="PLB46_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRF75293.1"
                     /translation="MSKRNKIIYWVSTLWLSLGMVSTGIVQLIQKQSEVDFITGLGYP
                     AYLLIIIGIWKILGVVVVLAPKLPLLKEWAYAGFFFVMSGAIISHIAVNNGIKEIAPA
                     VLLLVLTIVSWLFRPADRKLVIA"
     gene            5138..5716
                     /locus_tag="PLB46_01905"
     CDS             5138..5716
                     /locus_tag="PLB46_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRF75294.1"
                     /translation="MEKNIESYFSKPKKWSKEIQEMRAIALDCNLTETFKWGHPCYTL
                     NNNNIVLIHEFKTYCAYLFFKGALLKDSKGILIQQSENVQSARQIRFTNVEDIIRLKK
                     TIKSYIFEAIEIEKAGLKVALKETAAYTIPEEFQQVLDKQKKVHKAFYALTPGRQRAY
                     LLHFAAPKQSKTRVARIEKCLPLILNGLGLND"
     gene            5752..6309
                     /locus_tag="PLB46_01910"
     CDS             5752..6309
                     /locus_tag="PLB46_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HRF75295.1"
                     /translation="MKKQLTKEQRESLMMLLKTRFTKNMHRHKGLDWNNVEAKLIANP
                     EKLWSLNEMETTGGEPDVVKYDKKNDQYIFVDCSEESPKGRRSICYDYEALMSRKEHK
                     PKNNVIDMANDMGISVLNEAQYRELQEIEAFDKKTSSWVQTPSEIRELGGAIFCDYRF
                     GKVFVYHNGAESYYAARGFRGMLTV"
     gene            6363..6935
                     /locus_tag="PLB46_01915"
     CDS             6363..6935
                     /locus_tag="PLB46_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75296.1"
                     /translation="MQANHAPVKLWSQYFLIVLLTIFIHELGHCVVDWSLGIHAIPTP
                     AKTYALNSYPAYLEHKSTVGGILATLIIAVAGLIIYYRNPSRFNTVLLAGTIAMPVVY
                     SLLFTIKGRGHGDTEFQYAQEMLGFSFSGHALDWIFIALICCAFSLWILRSKPKLKAI
                     IPVIFGIVLTFIFVVVLQELNNFIFDPLFE"
     gene            6968..7303
                     /locus_tag="PLB46_01920"
     CDS             6968..7303
                     /locus_tag="PLB46_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF75297.1"
                     /translation="MKTKNSYFLLCILYASICGCADAVKACFTFSPDAPTVSAPVTFD
                     ASCSENSYLYIWNFGDGTVDTTVTQSVTVTHTYSSVGTYTVTLTAESKDGVTLRKGDF
                     TATQTVVIQ"
     gene            complement(7326..7616)
                     /locus_tag="PLB46_01925"
     CDS             complement(7326..7616)
                     /locus_tag="PLB46_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75298.1"
                     /translation="MTAQNKRLSGILLTVGVLLLFPLVAMQFSNGVDWSLFDFIIAGM
                     LLGGTGLLIELIMRKVKSKEKRLVLGALVLIVLALIWIELAVGIFGSPIAGS"
     gene            7909..8412
                     /locus_tag="PLB46_01930"
     CDS             7909..8412
                     /locus_tag="PLB46_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75299.1"
                     /translation="MVKIFIKIIIISLVFSACKQNPQKSPAEIKVAIQHELDKCVEAV
                     NSKNITLYMSLIPTDFELTNENGEIITREEQQAFILRDWSIIDTTLHNIYVVDSIDVY
                     TDSVIAFTSQKWERLMFQRDGVTKDTVITTQLHRETWRYKPTGWMNYGIIELGGEIYI
                     NGELYIE"
     gene            8445..8987
                     /locus_tag="PLB46_01935"
     CDS             8445..8987
                     /locus_tag="PLB46_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRF75300.1"
                     /translation="MSRKVILYIAASIDGYIAKPNNDLSFLNAVQREGEDYGYQEFMR
                     SVDTVILGRKTYDWVMTQVDIFPHAELQTFVITSSKRENSGKTIFYNGDIPELITNLK
                     KSEGLNIFIDGGAAVVNTLLNHKLIDECIISIIPVLLGDGIQLFNNGRPEQVLELLSS
                     KQYASGLVQLHYKMKGNKAL"
     gene            9206..9661
                     /locus_tag="PLB46_01940"
     CDS             9206..9661
                     /locus_tag="PLB46_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF75301.1"
                     /translation="MIEIKRTNSQNEDFIQLVAALDKDLAIRDGDEHGFYAQFNKIAN
                     LNYVVVVYNNQLPVACGAIKPYKPNCMEVKRMFVPEPERGKGWASMVLKELEKWAFEL
                     GTSKLILETGLKQPEAIALYRKNNYIVIPNYGQYANIENSVCFEKKINP"
     gene            9747..11081
                     /locus_tag="PLB46_01945"
     CDS             9747..11081
                     /locus_tag="PLB46_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75302.1"
                     /translation="MSQNRYFKFSLVMFICCNAFITTLNGQTTWHKYENNPVLQRDTV
                     IANLPNDLIAISDPWVMFEDGVYKMWYTCGGLNFPPDTLLRSRICYAISTDGINWEKY
                     EDNPVMDVDYSGSWDSLGVETVSIIIDNDAPASERYKMWYAGQYFNTYRYDIGYAYSP
                     DGINWTKHSTPVLSVGSSSEWDSGFLEGPSVIKEDGQYKMWYCGYDIVVNGQSTDGKA
                     NIGYAFSEDGINWTKYENNPIITTGANTWDSIYVQDPHVIKYGDEYQMWYGGGDNDAY
                     FSQQVGYATSTDGINWNKSPLNPVLTRGNDGDWDKILASFPSVIIKDGIYQMWYTGRN
                     LDPIPENSLDYYWELGLATAPFTALNNFIITDNIRLFPNPAQNNITLQFKNVQGETLI
                     TIYNNIGECLFTQHTSNIELLNIDLEQWANGMYLIAVQSDNIKELHKLIISK"
     gene            11097..11576
                     /locus_tag="PLB46_01950"
     CDS             11097..11576
                     /locus_tag="PLB46_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 domain-containing protein"
                     /protein_id="HRF75303.1"
                     /translation="MENAQQLAARLREVILNGRWIANTNYLDQLSDLSYEQANFKIGN
                     LNSIALLTFHINYYISGIAHFFKTGNLEIHDKYSFDMQPITSPENWLQLKNDLFAQTE
                     QLAQHITNFSVIQLEMVFVDERYGTYRRNIEALIEHAYYHLGQITLIKKMVITNATT"
     gene            11607..11996
                     /locus_tag="PLB46_01955"
     CDS             11607..11996
                     /locus_tag="PLB46_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF75304.1"
                     /translation="MHTETKAYNQSQSTADAAICQLLAELIDANLPKAENKIWHAHPV
                     WFLDGNPIVGYSKLKESVRLLFWSGQSFDEPGLSAEGKFKAAEKRYTDVEQVKAAEIK
                     RWLKKAAIIQWDYKNIVKRKGQLERLK"
     gene            12155..12616
                     /locus_tag="PLB46_01960"
     CDS             12155..12616
                     /locus_tag="PLB46_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF75305.1"
                     /translation="MKAIVQQLEKNDLEAFRSLIQIYVDIFGMEDYQPIADSNLLNAL
                     QQTNFEVFVATINNKVVGGLTIYILQPYYSAKGIAYIYDLGVAEQFQRMGIGKELLNH
                     LKTFCATNNLEEMYVQAEAIDRHAINFYRGTNPSSEMEVVHFNYIIGPENK"
     gene            12631..12990
                     /locus_tag="PLB46_01965"
     CDS             12631..12990
                     /locus_tag="PLB46_01965"
                     /inference="COORDINATES: protein motif:HMM:NF025161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HRF75306.1"
                     /translation="MIIITHHIQHKKIAEIGDDVIIIHNEDDGVQLLGEIYYAGYDAI
                     IMYEHQLTPAFFDLKNGLAGAILQKFTNYKMQLAIIGNFSATQSNSLRDFIRESNKRK
                     HINFVASLSDALLLFSN"
     gene            13096..14115
                     /locus_tag="PLB46_01970"
     CDS             13096..14115
                     /locus_tag="PLB46_01970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRF75307.1"
                     /translation="MEIGIDSFAAAPRNKEISHAQALNELLDRITFADEVGLDIFGLG
                     EHHRKEFLDSASHMLLAAAAAKTKRIILSSAVTVVSAADPVRIFQNFATLDLISNGRA
                     EIVAGRGSFIEAFPLFGFSLSEYDELFIEKLDLLLKIRDNEFITWKGKYRPALNNQAI
                     YPRPVQKKLPIWLGVGGTPESFIRAGELGLPLMVAVIGGETHRFRPLIDLYREAGTNA
                     GYGDDELTVGLHSLGFVANTSEEAVNAYYPGYAEMFTQIGKERGYPPVTKSRFDAQNG
                     PTGALLVGNPEEVGEKIIRHSKALGGISRFTFQMDAGLTHQQLMEAIELIGTKVIPAV
                     KNLLK"
     gene            14156..14752
                     /locus_tag="PLB46_01975"
     CDS             14156..14752
                     /locus_tag="PLB46_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75308.1"
                     /translation="MKSNKLFLFIVGLIIFISYSFSLKPDQFSELLTRSELIFTQPEN
                     FTAIEIIDQNTMQYEYALKHNTKNFEVRYAIRPLDELIKTYEKKEKNKKEGDINIHPN
                     KLYETLLQSTTMNISGGYLPEIVVFDSLAVRQEFNADWGATTYVEVGNAFGANYKYCI
                     TVAIHKNNVADAYLFFLSDNTENFDDVMLSAFHSLKFK"
     gene            14784..15143
                     /locus_tag="PLB46_01980"
     CDS             14784..15143
                     /locus_tag="PLB46_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HRF75309.1"
                     /translation="MGIQSIRPFIGAQNFELSCAFYRELGFNEEKLEEKLMVYKSGNF
                     AFYLQNAYVKDWVDNTMVFMEVDDITAFHSSLTVLNLPSKYQQVRLSEIRLFDWGKVV
                     YLHDPSGILWHIGEFKV"
     gene            15422..16879
                     /locus_tag="PLB46_01985"
     CDS             15422..16879
                     /locus_tag="PLB46_01985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF75310.1"
                     /translation="MQNKTVYTILFLVVVLSFFMHFSHFSDELIGTHVWRQTQTQSTI
                     INFYEEDMNIFNPRGNIKSDNDGIQRMEFPLMQWLVAGLYHITGNHLAVTRYFMFLIG
                     VIAMFGLYHLLLALFNKPILSLIGAWAFTFSPAFYYYTINPLPDNLALCCGIWGLALF
                     FKYVHNQKTIGLIASGLLMSLSALCKLPFILYYIVPIIYFIQIIRKQGNTFITLKTAM
                     LQLAWGLLPLVWYAVVIPQWSHNPVLAGVFDSQVSVLKLLDYLQHNIFITTPDLLLNY
                     GSVLFFIAGFYFLVKRKSYRDFRFIPILWLGIMVIAYYLFEIIPIGKIHDYYLFPFYP
                     LLFILVAYGAYHILYGSNKKLRIVSYVLLIVLPLTCFLRADTRWNNNNPGFNRDLLTY
                     KTELRNAVPKNAVVVAGNDISKVIFLYYIDKKGYPFLNNILTTNDLQGFVSGGAEYLY
                     TDSPDIIDQQQLTPFIDSLMATYGSVIVYKLKNPN"
BASE COUNT         5932 a         2849 c         3113 g         5713 t
ORIGIN      
        1 aatgcttcag acccattaaa ccctagtttc gcactttccg gatttgacaa tgaactatgg
       61 gagaaaatta atgccgttat agaataaatt caccctactg cgtttattaa gtttagttaa
      121 taaaatggca agaggtgttt aattttttat ttttaccgga atatatttta ccgatataaa
      181 attacgttat atcctatgcg ttttaaacat ctatttacaa gcatttgttt cgtgttgaca
      241 ttctctgccg gctttactca atcaatagat attagtggca cctgggtatt aaatttggaa
      301 aaaagtcaac tcgaagcaaa atccgaaggt tttaccggaa gtaaatttat tattagccaa
      361 aagggtgaaa agttttattt aacccgttat cactattatg gtgaaaggtc aaataaaatt
      421 agttttaaaa tgaaagccga tggaaaaacc cgaccagtta aactattatt cagaggtaaa
      481 ttggaacccg cgccaactac tttaaaagct tccatctgga aaaacaattt ttccaatatt
      541 gtagattact ccttcggctc atcgcaaaat gaattaattg ccgatgaagt gatgaaaagt
      601 aaacatgaca atcaccataa tatttgggta ttcgataaag tgattattga aaccactaaa
      661 taatacaata ttgcattgaa ttgttatctt ttacaaaata agtatggtat tacaatttag
      721 aaccggaaca atttcagata tccctcaatt acaacaattg gcaattagtt catgggggca
      781 atatgaaaaa gtactttcac ctgatgactg gaataaatta ttcaccaacc tttctaatcc
      841 tgatacctat gccgaattac tcgatatagc acattgtgaa gtttgtacta atgaaaataa
      901 taccatcatt ggaatggctt ttttagtgcc gagtggaaat cccagtgata tatatgattc
      961 ttcctggtgt caacttcggt ttgtgagtgt tgacccaaat tatagtggat ttggaatagc
     1021 caaaacctta acccaaaaat gtattactct cgcacaacaa aacaaggaac aaataatggc
     1081 attgcataca tctgaaatga tggataaggc aagacatatt tatgaatcgt tggggtttac
     1141 tatcttaaaa gaaattccac cacggtttaa taaaaaatat tggttataca cccttaaatt
     1201 ataggtaatg caatggcatg gcagcattaa cacaaaccta cttttgattt cgctctgtgc
     1261 aatgcttcaa tataaataca aaacaggttt gcagttgatt aagtaaataa ttggaaatag
     1321 gataccacta gtcaaaaaag atggacccgg agtggacgga gttgtgtttg agatggaaat
     1381 ttgagacatg ctaatctttg ccctgctgtc acaattttta aacattctga ttattgtgat
     1441 atttttgcct tctttaaact aataactgcg ctagttaagt tatgactaaa aaaccaagat
     1501 ttaccggtct gaataaattc ctcctagaat ttttatccat atttattggt gtattcgccg
     1561 cctttgcttt agataattgg aatgaagaca gaaaggacca tatcacggag atgagcattt
     1621 tgcaggaaat caacaatggc ttaaaacaag atttattgga tattcaggta aatacaaaag
     1681 ggcatgaata tggcatcatc gcaatccaac attttagaaa tattgtttta aatgttccgg
     1741 taaacaatga ttctattacg ttccattatt ttaatttatt ccgtgacttt gtttccgttc
     1801 aaaataattc aggttacgaa acacttaaat cgaaggggtt agaaatcatc agtaatgatt
     1861 cgcttcgttc caaaattatt gccttatacg agggcgatta taatactatt cgtaaattag
     1921 aagaagagta tgatgagtta caatttcagg aaaattatta caaagatttt aatactaaaa
     1981 tgtcaccttt ttaccaactg gacagtttag gaacattaaa aggtgtttta tctccggtaa
     2041 atttatctac gccggataag aatcaaattt taagtgattt ttggaaaatg caaaacaacc
     2101 gggtatttat tatacagtat tacctggagg ttgaaaagag aattaaagca ttacagactg
     2161 aaattaataa agaattgcat ttaacgcttg gtaatgctaa ttcatgctca gcaccttaaa
     2221 ttatcaaatt gattgtatct ttaagaaggc taaagtagca tcatgcgtaa aaattataaa
     2281 cttattgctg aaacattaat agttttcctg ttaaacggtt tattggcaat tgttttattt
     2341 gaatttatat caaatgattt gctaagatgg tcattgtaca tgcctttgct gattttccag
     2401 ggtttatggt attatcgttt gtatatcatc ggacatgaat gtgcgcataa aaagttaatt
     2461 cctgaaaata ataccgtgaa cgatatagtt ggcagtgtag cactaattcc attattggtg
     2521 ccaattaaca tctatcgtaa aatacattat tttcatcacg ggtttaatag aaaagaccca
     2581 catacatctg cattagacac ctttgtaata accggtaaat tcaaaaaggt gaaaactgtt
     2641 tattattatt ttatctggta tttaagtgtg ttttttggtg gtttctttct gcattcgctt
     2701 atctccgtaa tgttgttttt atttgtacct tcaaaattgt cttctagaat ttcaccggca
     2761 tttaaagggt ggcaaatgaa ggatcaattt gtagcgatac tgttattttg tattgggtta
     2821 ctgtttcatt tcgcaaccta tcaattgctt ggcaaacagt attatttatt ggttttagga
     2881 tttcccatgt tgtcctttgc ctgggtgctc tcactgttag tgtatatttt tcattatgat
     2941 accactataa gtgaagaggt tcgttttaat gtgcgctctg tgcgtccggt tccggttatt
     3001 tcatggatat taatgaactt taatgaacac gcaacacatc atcagtatcc aaatattccc
     3061 tggtatgaat taccctcaaa acgaaaacaa ttaccagaag catatgcact aaaaaatcaa
     3121 cggacaacca atttttttgt tgcaattttc aggcaattaa aaggtccttc cattcattat
     3181 gaaaattaat attcgttatg ccaaattgga agacgctgta gcaatggttg aaatcaaaaa
     3241 tcaactgtcg tttaaaaaca ttaatggcac cactacaaca ggaggttttt tactgggaac
     3301 aactttacaa acctatcagg aatacattgc aaacgattat tgtttagttg cagaaaatga
     3361 gtaccaaatt attggctttg gcattatatt gaaaaatacc actttacaac aatcagatat
     3421 ttggactaaa cggcatgctg caaactggga aatagatatt accaaatacg aatcaccaaa
     3481 tgtatgctac ttcgagcaat tagctttttt acgcggttac accagagtgg taattcgttt
     3541 atgttatcaa ttactacagc ttgcatttaa cacgcacgat aatttgttta cgacaacagt
     3601 ttcatctccc attttgaact tagcagcaat cccctttatc gaaaaagccg ggggcagaaa
     3661 aattgggtgt attaatgaag tgtatcctga aatcggactt atactttctg acatctacct
     3721 gatagagaaa gctatttata ttaaacgact ggaacattcc ggcctccaac ctttactgaa
     3781 caggatacca tacgagttac aaatatttta aaaaaaataa attttgtaac cggcacaaaa
     3841 tcaacacttt atacctccaa gttctgaggt tttactccat tatcaaaaaa aatagtggta
     3901 aaaattttgc gtagtcattt aactacatat atatttgtag tcaaatagct acatatgaat
     3961 ttacgcagag atgtatttca agccattgcc gacccaacca ggcgaggaat tttgttgctt
     4021 gttgcctcac aagctatgac agcaggagca atagcagcca attttgatac tgcaaggccg
     4081 acagtttcca aacatctgca tattttaact gaatgtgaac ttctcactca agtgcaagac
     4141 ggaagagaaa tttattataa aaccaacgtg aaaaaaatga aagcagttgc cgattttatt
     4201 gaaccattcc gcaaaatgtg ggacgaccga ttcaacaaat tagaagaagt aatgaaaaaa
     4261 cgacataaaa aataaactac tatggaactc aaaactaaaa tacatgctga agatggtaag
     4321 caggaattgt ttatcaccag aaactttgat ctaccggttg aactattatt tgaagcttat
     4381 acagatgcag aaattgtcgc ccaatggatg aataccaatg tattaaaact ggagaataaa
     4441 aatcatggca gttacttgtt tgaaacaacc gaccctatgg gcaataaaca tcggttcaat
     4501 ggcaccatac atgaaatgca aaccaatgca tttattacac ggacattcca aatggaaaac
     4561 acagcttttc ctccacaact cgaattttta gtattcgaaa aaataacgga tgattccagt
     4621 aaactcacta tgcatgtagt ttaccaatct gtagctgtaa gggaccaaat attacaattg
     4681 ccattcgcta aaggtattaa tatgggacat aatcgtattc aggaaatatt atctaaaaac
     4741 aactaatatg tcgaaacgca acaaaatcat ttattgggta agcacattat ggctatcgtt
     4801 aggtatggta tctaccggta ttgtgcaatt aattcaaaaa caatcggaag tagattttat
     4861 taccggcttg gggtatcccg catacctgtt aattataatt ggcatctgga aaatattggg
     4921 tgtagttgtt gttctagctc caaaattgcc gctacttaaa gaatgggctt atgcaggatt
     4981 cttttttgtt atgtcaggag ccataatttc tcatattgcg gtaaacaatg gtataaaaga
     5041 aatagcgccg gctgtattac ttttggtgtt aacaatagta tcgtggttgt tcagaccggc
     5101 agacaggaag ttagttattg cttaaagtgt aattataatg gaaaaaaata ttgaaagcta
     5161 tttcagcaaa cccaaaaagt ggtcaaaaga aatacaggaa atgcgcgcta ttgctttaga
     5221 ttgcaacctg actgaaacat ttaaatgggg acatccatgt tatactttaa ataataacaa
     5281 tatagttttg attcatgagt tcaaaactta ttgtgcctat ttatttttta aaggcgcgct
     5341 tttgaaagac agcaaaggaa tacttattca acagagtgaa aatgtgcagt cagcacgaca
     5401 aattcgtttt acaaatgttg aagatattat tcgcttaaaa aaaaccatta aatcttatat
     5461 ttttgaagca attgaaattg aaaaagcagg actaaaggtg gctttaaaag aaacagctgc
     5521 atataccatt ccggaagagt ttcaacaggt attggataaa caaaaaaaag tgcacaaagc
     5581 attttatgca ttaactccgg gcagacagcg ggcttatctt ttacattttg cagctcccaa
     5641 acaatccaaa actagggtag caagaattga aaaatgttta ccccttattt taaatggatt
     5701 gggattaaat gattaaaatt atattcgtta actgatatca actataaggt tatgaaaaag
     5761 caacttacaa aagaacaacg cgaaagttta atgatgctat taaaaacgcg ttttacaaaa
     5821 aatatgcatc gacataaagg tttagactgg aataatgtgg aggcgaaatt aatagcaaat
     5881 cccgaaaaat tatggtcgct gaatgaaatg gaaacaacag gtggtgaacc cgatgtggta
     5941 aaatatgata aaaaaaatga tcaatatatt tttgtcgact gcagtgaaga gagccccaaa
     6001 ggcaggagaa gtatttgtta tgactacgaa gcacttatgt cccgtaaaga acataaacct
     6061 aaaaataatg tcatcgacat ggcaaacgat atgggaataa gtgttttaaa tgaagcgcaa
     6121 taccgcgagc ttcaggaaat tgaagcattt gataaaaaaa cttcaagttg ggtgcaaaca
     6181 ccatctgaaa tcagagaatt aggtggtgca attttttgtg attatcggtt tgggaaagtg
     6241 tttgtgtatc acaatggcgc tgaatcgtat tatgctgcga gagggtttag gggaatgttg
     6301 acagtgtaaa ttatttactg tttgaaaaaa aagttatgta agtttaaatc ataattggca
     6361 taatgcaagc gaaccatgca cctgttaaat tgtggtcaca atattttctt atagtgttgt
     6421 tgaccatttt tattcatgaa cttggacatt gtgttgtcga ctggtctctt ggcattcatg
     6481 caattcctac tcccgctaaa acatatgctt taaatagtta tcctgcatac cttgaacata
     6541 aaagcactgt tggaggtatt ttggcaactt taattattgc agttgccgga ttaattattt
     6601 attatcgcaa tccttcgcgg tttaacactg ttttattagc aggtacaatt gcaatgcctg
     6661 tggtgtattc gctattattt acaataaaag gaagaggaca tggcgacacc gaatttcaat
     6721 acgcgcagga aatgttaggc ttcagttttt caggtcatgc acttgattgg atttttatag
     6781 ccttaatatg ttgtgccttt tcactttgga ttttacgttc caaacctaaa ttaaaagcca
     6841 taattcctgt tatttttgga attgtattga catttatttt tgtagtagtt ttacaagaat
     6901 taaataactt tatatttgat ccattgtttg aataattata tcaaataatc aatagccccg
     6961 attctaaatg aaaactaaaa acagttattt tctgctttgt attttatatg caagtatttg
     7021 cggatgtgct gatgcagtaa aagcatgttt tacattttca ccggatgcac caactgtgtc
     7081 cgcaccggta acctttgatg caagttgttc agaaaatagt tatttataca tctggaactt
     7141 tggtgatggc acagttgata caacagttac tcaatcagta acggtaaccc atacttactc
     7201 aagtgtaggc acttataccg ttacattaac tgcagaaagt aaggacggtg ttacattgcg
     7261 taaaggtgat tttactgcta cacagacagt ggtaatccaa taattggtag ttatgttttt
     7321 aggttttaag aacccgcaat cggtgaacca aaaatgccaa ctgcaagttc aatccagatt
     7381 aatgcaagta caattaacac caaagctcct aataccaacc ttttctcttt tgattttact
     7441 ttacgcatga tcaattcgat taataacccc gttcccccta acaacatacc cgctattata
     7501 aagtcaaaga gtgaccaatc cacaccattt gaaaactgca tggctaccag aggaaacagt
     7561 aataaaaccc ccactgtgag taggattccg gataatcttt tgttttgagc tgtcataatc
     7621 tgtttgttta ggatacaaat gtatttaaaa gtgctttgtt tttcaaagta attaataaac
     7681 tttaactttt cccctaaaca acaccccttg ggcacttagc taaggaatgc gtttttcaat
     7741 gtccgaaata cgataaagcg tatgccacgt gcaaaaagtt ttacctaact ttccattgat
     7801 atttttttaa tgcaccattt tttgtatcaa acattagctt tttgcgtagc cacgcaggtt
     7861 acctttttaa cattctaaat agtactttta ttatcgaatt aaattaatat ggtgaaaata
     7921 tttattaaga ttattattat cagtttagtt ttttctgcct gcaaacaaaa cccgcaaaaa
     7981 tcacctgcgg aaataaaagt tgccattcag catgaactcg ataaatgtgt agaagcggtt
     8041 aattcaaaaa atataacatt gtatatgagc ctgataccta cagatttcga attaacgaat
     8101 gaaaacgggg agataattac acgtgaagaa cagcaagctt ttatattacg cgactggtct
     8161 ataattgata ccacactgca taatatttat gtagtagata gcattgatgt atatacagat
     8221 agcgttattg ctttcacgtc tcaaaaatgg gaaaggttga tgtttcagcg cgatggagtg
     8281 acaaaagata cggttattac cacacagtta catcgcgaaa cctggcgtta taaaccaaca
     8341 gggtggatga attatggcat tattgaatta ggtggtgaaa tttatatcaa tggtgaattg
     8401 tatattgaat aagcagattt tttaataaat ggaaccatta atgtatgagc agaaaagtga
     8461 tactatatat agcagctagc attgatggtt acattgctaa accaaacaat gatttaagtt
     8521 ttttaaatgc cgtacaacgt gagggtgaag attatggtta tcaggaattt atgcgttcag
     8581 tggacaccgt tattctaggt cgtaaaacat atgattgggt tatgactcag gtggatatat
     8641 tcccacatgc cgagttgcaa acgtttgtaa tcactagcag taaacgagaa aattcgggta
     8701 aaaccatatt ctataatggt gatataccgg aattgataac caaccttaaa aaaagtgaag
     8761 gcctaaatat atttatagat ggtggtgcag ctgtggtaaa tacattatta aatcacaaat
     8821 tgatagatga atgcatcatt tctataattc cggtgctact gggtgatgga attcagctat
     8881 ttaacaatgg cagacccgag caggtattgg aattattatc cagtaaacaa tatgcatccg
     8941 gactagtaca gttgcattat aaaatgaaag gaaataaagc cttgtaattt accgattgtt
     9001 tgtgcctgag gatgctaaaa tcttatcgta ggtttttata ttatctcctt ttcccgatat
     9061 aactttgtgt ttaatttgac ggttaaaatt ttacctattt atacgaataa tttataccga
     9121 taaactgttt gtaaataatt attgttaagt attaccgtta actttatata ggcatttact
     9181 attacttttc atttgcaact ttttcatgat tgaaataaaa aggactaatt ctcaaaacga
     9241 agattttatt caactagtag ctgcattgga taaagattta gccattcgcg atggagatga
     9301 acatggtttt tatgctcagt ttaacaaaat tgccaatctc aattatgtag ttgttgttta
     9361 caacaatcaa ttaccggttg cttgtggagc tataaaacca tataaaccaa attgtatgga
     9421 ggttaaacgc atgtttgtgc ctgaacctga acgcggaaaa ggttgggcat caatggtttt
     9481 aaaagagttg gaaaaatggg cgtttgaact gggtacctcc aaattaattt tagaaaccgg
     9541 ccttaaacaa cctgaagcaa ttgcactcta tcgcaaaaat aattatatcg ttattcccaa
     9601 ttatggtcaa tatgcgaata ttgaaaacag tgtttgtttt gaaaaaaaga taaacccata
     9661 agacagactt ctaatttata cattttgaaa tgttgggaat acaatatttc ctctgttttt
     9721 gtaattttat ctaatatctc gctattatgt cgcaaaatag gtatttcaaa ttcagtttag
     9781 taatgtttat ttgttgtaat gcatttatta caacattaaa cgggcaaaca acatggcata
     9841 agtatgaaaa caacccggtg ttacaacggg acacagtaat tgccaatctc cccaacgatt
     9901 taattgcaat cagcgaccct tgggtaatgt ttgaggatgg tgtatataaa atgtggtata
     9961 cctgcggtgg tttaaatttc cctcccgata ctttattgcg ttcaagaatt tgttatgcca
    10021 tttcaaccga tggcattaat tgggaaaagt atgaagacaa tccggttatg gatgtagatt
    10081 attcaggtag ctgggattct ttaggtgttg aaaccgtttc cataattatt gataatgatg
    10141 caccagcttc agaaaggtat aaaatgtggt atgccggtca gtattttaat acgtatcgat
    10201 atgatatcgg ttatgcatat tcacctgatg gtataaattg gactaaacac tcaactcctg
    10261 tgctaagtgt tggcagctct agtgaatggg atagcggttt tttagaaggt ccttcagtaa
    10321 ttaaagaaga tggtcaatat aaaatgtggt attgtggtta cgatattgtt gtgaatggcc
    10381 aatcaactga tggaaaagca aatattggtt atgccttttc tgaggatggc attaactgga
    10441 cgaaatatga aaataatcca ataattacta caggagctaa tacgtgggat agtatttatg
    10501 tgcaggatcc tcatgtaatt aaatatggcg atgagtatca aatgtggtat ggcggtggtg
    10561 ataatgatgc ctattttagt caacaagttg gttatgcaac ttcaactgat ggaattaact
    10621 ggaacaaatc tcctttaaat ccggtgctta caagaggtaa tgacggagat tgggataaga
    10681 ttttagcatc attcccttca gtaattatta aagacggcat atatcaaatg tggtacaccg
    10741 gtcgaaattt ggaccccatc cctgaaaatt cattagatta ttattgggaa cttggattag
    10801 ctacagcacc ttttacagca ttaaataatt ttattattac agataatatt cgtttattcc
    10861 ccaaccctgc acaaaataat attacacttc aatttaaaaa cgtccagggc gaaacgctga
    10921 ttacgattta caataacatt ggtgaatgtt tatttactca acataccagc aatattgaat
    10981 tattaaatat tgatttagag caatgggcta atggcatgta tttaatagct gtacaatcag
    11041 ataatataaa agaattacat aaactgatta tatcaaaata atataattac aataatatgg
    11101 aaaatgctca gcaattagcc gctcgattgc gtgaagtaat tttaaacgga cgttggattg
    11161 cgaataccaa ttacttggat caactatctg acctcagtta cgaacaagcg aattttaaaa
    11221 ttggtaattt aaattctatt gcgcttttaa cgtttcatat caactattat atttcaggca
    11281 tagcacattt tttcaaaaca ggtaatctcg aaatacacga taaatatagc tttgatatgc
    11341 aaccaataac ttcaccggaa aactggttgc aactcaaaaa cgatttattc gcgcaaacgg
    11401 aacaactggc gcaacacata acaaattttt cagtcatcca acttgaaatg gtgtttgtag
    11461 atgaaagata tggcacttat cgtcggaata ttgaagcatt aattgaacat gcctattatc
    11521 atctggggca aattacattg ataaaaaaaa tggttattac caatgcaaca acctaatata
    11581 taatcaccga atcaaactat cattttatgc ataccgaaac aaaggcctac aatcaatcac
    11641 aatcaactgc tgatgctgct atttgccaat tattagctga gcttatagac gccaatttgc
    11701 ctaaagcaga aaataaaatc tggcatgccc accctgtttg gtttttagat ggcaatccaa
    11761 ttgttggtta cagcaaatta aaagaaagtg tacgcctgct attttggagt ggacagtcat
    11821 tcgatgaacc gggtttatca gctgaaggca aatttaaagc tgcagaaaaa cgatataccg
    11881 atgttgaaca agttaaagct gctgaaataa aacgatggtt aaaaaaagca gcaatcattc
    11941 aatgggacta taaaaatata gtaaaacgta aaggtcaact ggagcggtta aaataatctg
    12001 aaataattta aattaacaaa ctatttggct ttgtaaatta aaacataacg cttgctatct
    12061 gagctaagct gaataaatac agccactttc tggctcatca aataacttcc acgttaatag
    12121 gttatcttta taaaaaattt acccttttaa ttgtatgaaa gctattgttc aacaacttga
    12181 aaagaacgac ctagaagctt tcaggtcact cattcagatt tatgtcgaca tattcggaat
    12241 ggaggattat caacctattg ctgatagcaa tttactcaat gcgcttcagc aaactaattt
    12301 cgaagttttt gttgcaacta taaataataa agtggtaggt ggtttaacca tttatatcct
    12361 ccaaccctat tattccgcaa aaggtattgc ctacatttac gatttgggtg ttgcagaaca
    12421 atttcaacgt atgggaattg gtaaagaact cctcaatcac ctgaaaacat tttgcgcaac
    12481 aaataattta gaagaaatgt atgttcaggc agaagcaatt gaccgccacg ccattaattt
    12541 ctacagaggc actaatcctt caagtgaaat ggaagtggtt cactttaatt atatcatcgg
    12601 ccctgaaaat aaataggaca ctttagtcag atgattatta ttacgcatca tattcaacat
    12661 aaaaaaattg ccgaaattgg tgatgacgtg ataattatcc acaatgaaga tgatggcgtt
    12721 cagttgctgg gtgaaatata ttatgccggt tatgatgcaa tcattatgta tgaacatcag
    12781 cttactcccg ccttttttga tttgaaaaat ggtttggccg gagccatcct gcaaaagttt
    12841 accaattata aaatgcaact ggccattatt ggtaattttt cggccacaca atcaaatagt
    12901 ttacgtgatt ttatcaggga aagtaataaa cgtaaacata tcaattttgt agcatcatta
    12961 tcagatgcat tactattatt ttccaattaa gccgcaacct tctatttaat tatatattgg
    13021 tatcctattg ttatatttgg caattcgtta catcgaacca aagattaact ttgttggtta
    13081 ttgaaaaaaa atacaatgga aataggaata gatagttttg ccgcagcgcc tagaaacaag
    13141 gaaattagtc atgcacaggc gctaaatgaa ttattagacc gcatcacatt tgcggatgaa
    13201 gttgggctgg atattttcgg cttaggtgaa catcacagga aagaattttt agattctgca
    13261 tcacatatgt tgcttgcagc ggcagcagct aaaacaaaac gaataatatt aagcagcgca
    13321 gttacggtag tgagtgcagc agaccctgtt cgcatatttc aaaattttgc aacattagat
    13381 ttaatatcca acggtcgcgc agaaattgtt gcaggtcgtg gttcatttat tgaagctttc
    13441 cctttatttg gttttagttt aagtgaatac gatgaattgt ttattgaaaa actggacctc
    13501 ttattaaaaa ttcgggataa tgaatttatt acatggaaag gtaaataccg acctgcgcta
    13561 aataatcaag ctatttatcc tcgacctgtc caaaaaaaat tacctatttg gttaggtgtt
    13621 ggcggaactc ccgaatcatt tatccgtgcc ggtgaattgg gattaccatt aatggttgcc
    13681 gtaattggtg gcgaaacaca tcgttttcgt ccactcattg atttatatcg cgaagcagga
    13741 acaaatgcag gttatggaga tgatgaatta actgtcggat tacattcgtt aggatttgta
    13801 gccaacacaa gtgaagaagc agttaatgcc tactatccgg gttatgcaga gatgtttaca
    13861 caaattggaa aggagcgcgg atatcctccg gtaaccaaat cgcgttttga tgcacaaaac
    13921 ggccctacag gcgctttatt agttggtaat ccggaagaag ttggagaaaa aattattcgt
    13981 cacagcaaag cattaggtgg catttcccgt tttacttttc aaatggatgc agggttaacg
    14041 catcaacaat taatggaagc aattgaactt attggtacta aagtaattcc agctgtaaaa
    14101 aacttattaa aataaaacaa caaattatta atcattaact tctgatggtc aagaaatgaa
    14161 atcaaataaa ctttttcttt ttattgttgg gttaattata tttatcagct attctttttc
    14221 cttaaaaccg gatcaattta gcgagctatt aacgcgtagt gaattaatat ttacccaacc
    14281 tgaaaatttt actgcaattg aaataataga ccagaatacg atgcagtacg aatatgcttt
    14341 aaaacacaat accaaaaact tcgaagtgcg ttatgccatc aggcctttgg atgaattgat
    14401 taaaacatat gagaaaaaag aaaaaaataa aaaagaaggg gacatcaata ttcatcccaa
    14461 taaattatat gaaacattat tacaatcaac aacaatgaat atttccggcg gctatttgcc
    14521 tgaaatagtg gtgtttgatt ctcttgctgt aaggcaggag tttaatgccg attggggcgc
    14581 aactacgtat gttgaggtag gcaatgcatt tggtgccaac tataaatatt gtattactgt
    14641 tgccatacac aaaaacaatg tggcagatgc ttatttgttt ttcctctccg acaatacaga
    14701 gaattttgat gacgttatgt tatccgcttt tcattcttta aaatttaagt aatttccgct
    14761 ttgagaaaat aaaattataa aatatgggca tacaatctat tcgaccattt attggcgcac
    14821 aaaactttga attatcctgt gcattttatc gcgaactggg ctttaacgaa gaaaagttgg
    14881 aagaaaaact aatggtatat aaatcaggta acttcgcgtt ttatttacaa aatgcatacg
    14941 taaaagattg ggtggataac accatggtat ttatggaagt agatgatatt actgcgttcc
    15001 acagctcgtt aactgtatta aatttaccct ccaaatatca gcaggttcgt ttatcggaaa
    15061 ttcgattatt tgattggggg aaagtagttt atttgcacga tccatcaggg attttatggc
    15121 atatcggtga atttaaagtt taatccaaag tagatttatc tccgggcaca attctcattt
    15181 gtgtaataat tgtcaatacc taaactgtat ttacatcata aacagattac agttttggta
    15241 atttagttta tcaaggaatt tagtactttt taattgttat atccaacgct caatgtagtt
    15301 ttgcagttaa ttttgtagat gtttcgaatt aagtggcatg ttcgcctgat tcaaattgtt
    15361 taaatttaca ggtatcaatt gcaagctaga acgataaggg ggcatgatac caatacaaaa
    15421 attgcagaat aaaacagttt ataccatttt atttttagtg gttgtgctta gtttttttat
    15481 gcacttttca cacttctcag acgagttaat tgggacacat gtgtggcggc aaacgcaaac
    15541 acaatcaacc attattaact tttatgaaga ggatatgaat atttttaatc ccagaggtaa
    15601 tatcaaatcc gataatgatg gtattcaacg aatggagttt ccattaatgc aatggttggt
    15661 agccgggcta tatcatataa caggcaatca cctggcggta acaagatatt tcatgttttt
    15721 aattggcgta attgccatgt ttggtttata tcatttgttg ctcgcgttat ttaataaacc
    15781 aatattatca ttaattggtg cttgggcttt tacgttttca cctgcatttt attattatac
    15841 cataaacccc ttgcccgaca atttggcact ttgttgcggt atttggggat tggctctttt
    15901 ttttaaatat gtccataatc aaaaaacaat tggtttaatt gcctcaggct tattaatgag
    15961 tttgagtgca ttgtgtaaat taccttttat actctattat attgttccta ttatttattt
    16021 tattcaaata ataagaaaac agggaaatac attcatcacc ttaaaaacgg ctatgctgca
    16081 attggcttgg ggtttactgc cattagtctg gtatgctgta gtgataccac aatggagcca
    16141 caatcctgtt ttagcgggtg tgtttgatag ccaggtttca gtattaaaat tgttagatta
    16201 tttacaacat aatatattta ttaccacacc cgatttatta ttaaactatg ggtctgtgtt
    16261 attttttatt gctggatttt attttcttgt taagcgtaaa tcatataggg attttcgatt
    16321 tatccctata ttatggctcg gaataatggt tatcgcatat tatttatttg aaattatccc
    16381 cataggaaaa attcacgatt attatttatt tccattttat cctttgttgt tcatcttagt
    16441 tgcttatggt gcctaccata tactttatgg aagtaacaaa aaactacgta ttgtatctta
    16501 tgtactatta attgttttgc cgttaacatg tttccttcgg gcagatacca gatggaataa
    16561 taacaatccc ggttttaacc gcgacctgct cacctataaa acagaattac gcaatgctgt
    16621 accaaaaaac gcagtggtgg ttgcaggaaa tgatatatca aaagttattt tcctatatta
    16681 tattgataaa aaagggtatc cgtttttaaa taacatactt accacaaacg atttacaggg
    16741 atttgtgtca ggaggtgctg aatatttata taccgattca cctgatataa tagatcagca
    16801 acaactaaca ccatttattg attctttgat ggcaacgtac ggttcagtga tagtgtacaa
    16861 acttaaaaat ccaaactaaa ttgtttagct agttgaattt ctgattaaca caactcaatg
    16921 tgtttcagga atttattatg ttacaattga tgatgcaaat atgttgatag cgcaatctaa
    16981 tttagttatt gtgaattaat aaaaagtata taaagtgtaa tgcaataagt tgctcgttga
    17041 gttaatcaca aagttcattc actttaatga ttctaaaaaa caatttctaa attcaaacaa
    17101 tactgtatgt ttaaaataaa aggttggtca ctttgatata cactttgcgc aaaaaacgat
    17161 tttatagtta aactaaatat gttcatctat aagtccttat aagttattta gttgtagtca
    17221 ttttaagcta cttacttcaa gctcatccgc cagttaaacg aaagtggtaa tcccaacata
    17281 tataattttt attaatagaa ctaaaatgtt gaaaacttgt ggttttagcg tagttataag
    17341 gctgcatttt taaaggttgt accgagtttt ctgtattaat caacgttaaa tcagtacaga
    17401 attaacttga caattgttaa aaaattatat attatttgtg aagcaaccca ttcaaaccca
    17461 tatacatcaa tgagttagtc acatttgttt aaaacctggc cttggctgct taaataaacg
    17521 ttgctatttt tactgttgta agtatcccta tttattaccc aataccaccg attttatgtt
    17581 tctgaaaaaa ttaattccaa tagccct
//
LOCUS       DAORKW010000022        17119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_35_length_17119_cov_5.791256, whole genome shotgun sequence.
ACCESSION   DAORKW010000022 DAORKW010000000
VERSION     DAORKW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17119
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35_length_17119_cov_5.791256"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..3034)
                     /locus_tag="PLB46_01990"
     CDS             complement(77..3034)
                     /locus_tag="PLB46_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HRF75311.1"
                     /translation="MQSNSNYQVLIQKLDEFIRKFYLNQLIRGVLFTAAILVSAYLLF
                     SFLEYRFYFSTTVRKVLFYSFLGGAGFLLWNNIITPLLKYNRLGKTINHKTAASIIGT
                     HFSEIQDKLLNILELREQAEGTGNQLLIEAGINQKIEKIKPIPFSLAIDLNKNKKYLK
                     YALPPLGVLVFVIFAAPNVLKDSNLRLLKNNTFFEKAAPFNFLVQNKSLEVVQYEDFE
                     LKLQITGDILPEQVAIVTATGTYTMQKSDDDAFTYVFNKVPANTDFHFEANGYQSKER
                     TLTVVPKPTLLNFDINVNYPDYTGIKDERLQNTGDLLVPEGTNLEWLFNTENTDVIEL
                     FFNDQKMNAVKDGKSSFTSSRKAMQNEVYKIGIGNTQVYNRDTISYTITVIPDEFPQI
                     TAEQFKDSTDNKFLYFLGEINDDYGFKSMYFKYKVEGRDAADNYFVKEENKDVLSVPS
                     GVKSNRYTHSFDLRAKTLAPGDRVTYFFEVWDNDGVHGSKSTRTAAMQFVVPTLDELQ
                     DIKDEKNEDIKDKLEEVLDDIADVKKQTEKLEDKIFDKKELNWEDKKAIENLMQKNQN
                     IQQSVEEIKEQYKENLELQKEYLQPDPEILEKQEQLEKMFDEVIPEEMKKLMEELQQL
                     MEKMNKEQSLEEMKEMQMNNKQLEQELDRMLELFKQMEMEQKMQETIDKLNQLSEEQQ
                     KLAEQTENKQSKQEENLEKQEELNKDFDDVQKDMQEMREMNKELESPKELGNTEQQEQ
                     QIDQKMEQSKQQMQQNNKKQAAQEQKSAAQQMKEMAAQMQQQMQQQQQQQQEEDLEAL
                     KQLLDNLIKLSVDQESLMKEVKTLKSSNPKFIDLMAQQQKIKEDTKIVEDSLLALAKR
                     VTQISSFITREMSDVNTNLQNSVDQMGERQINQSNLYQQQTMTGYNNLALMLDEVLQQ
                     MQQQMAQSMPGSQQCQKPGGKNESQMPSMSEMQKQLNEQLNKMKGQNPQSQQKGGQSG
                     MSKELAEMAQNKP"
     gene            3209..3574
                     /locus_tag="PLB46_01995"
     CDS             3209..3574
                     /locus_tag="PLB46_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75312.1"
                     /translation="MDALTREELPIDFDPRWIAVLKQMKEQFGKKPELEAVLFLIGMN
                     ELGRVIEKFTKEEKQDLMHIAVCKLLSYDGFYRFAGWDDDNWPMWEAAEKLPQWTNED
                     QERAIKLNIIRYFEENNII"
     gene            3571..4305
                     /locus_tag="PLB46_02000"
     CDS             3571..4305
                     /locus_tag="PLB46_02000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF75313.1"
                     /translation="MKKTTLIAVCLMAFVACKEKTTPTPEEPKAEIQTTTQTNTMENQ
                     NDTTQYYVNISTSFGDMVVKLYNETPKHRDNFIKLVKDGYYNDLLFHRVIKDFMIQGG
                     DPASKNAGPNVQLGAGGPGYTIPAEFNRNFIHKKGALSAARQGDQVNPTKASSGSQFY
                     IVQGKVTDAAQLEQMGRQTGNSYTPEQTAIYSSIGGTPFLDNQYTVFGEVIKGLEVID
                     KIAAVKTAYGDRPQQDVKMTMTMVTM"
     gene            4472..5374
                     /locus_tag="PLB46_02005"
     CDS             4472..5374
                     /locus_tag="PLB46_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF75314.1"
                     /translation="MYSITTNNLNYRFNGKETVLSDINLQVPTGSIYGFLGPNGAGKT
                     TTLRLILGLLKKQDGEINIFNQPFEKNRIEILRKTGSLIESPSLYAHLSAKENLAILQ
                     KVYQCKPSRIDEVLKIVGLENTGKKSTGKFSLGMKQRLSIAIALMHEPELLILDEPTN
                     GLDPNGIIEMRELLKQLNKDFGMTILVSSHLLAEIEKMVTHVGIINKGKLIFQGTMGE
                     LHEAQHQQSFICFETGNIEKTRAVLNANGIQFQQEGSQLHVPVLERELIASINKQLVE
                     QDIKVYQISLIKNDLESIFIDLVK"
     gene            5380..6147
                     /locus_tag="PLB46_02010"
     CDS             5380..6147
                     /locus_tag="PLB46_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF75315.1"
                     /translation="MQLINSIKSEWLKTRKSAASWLCLIGGLFIPLIFLIGFLYKGET
                     LSTYGDAENVWTAHFFQVWQSMVVFLLPMGLILATSLITQMEFRNNTWKQLHTTPQRY
                     YVIFWAKFIVIIGMTLQFFFYFNLGFILSGIIPSLVVDKQLPDVIFPYSQFFTSNWKI
                     FVTCLPVLALQFLISLRFKNFLVPIGVGLMLLVATLILVGNWDHVYLSPFSYSPLTMM
                     GTPETMKNINIYNWSCYYFIAFMVISYLMYIRKTEKG"
     gene            6184..7239
                     /locus_tag="PLB46_02015"
     CDS             6184..7239
                     /locus_tag="PLB46_02015"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF75316.1"
                     /translation="MKKPVVILLHVGYWLVFLLLLFVLYGLSNAAALSNEQDPGVGAG
                     EWFKLMFSTTILPGVISFYTFYFIIFSRFLQKRKIPAFFISVFVASYVAAIIGGAVGS
                     INYFLGYHFLMDKNLPTALSMLTFLAFIALLNGIIGLVMRGFITWYSEIKMKEDLNKK
                     NFEMELALVKSQINPHFLFNTINNIDVLITKEPELASKYLNTLSDIMRFMLYETKAER
                     ILLSKELAYIEKFIGLHKIRSNNPDYVHFTVNGAAPNMMIAPMLFIPFIENAFKHVAD
                     KKANPAIVISLNIDKDKITFICQNNFKEETRDENEHSGLGDGLIRKRLQLLYPDKHEL
                     TVTTQNNIYAVYLTISS"
     gene            7243..7923
                     /locus_tag="PLB46_02020"
     CDS             7243..7923
                     /locus_tag="PLB46_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF75317.1"
                     /translation="MKCIIVEDEPLALERTKGYVEKLPFLKLLATFDNGVDALLFLKD
                     NTIDLLFLDINLGELSGIQMLEAAKPDCAVIITTAYHEYALKGYELNVTDYLLKPFTF
                     ERFFQAVEKAKVSLDKKEVPEKKSVFIKTEYRLEQVLLTDIIYIEGMRDYRRIFLHGK
                     SIMTLQTFKELEEMIPPAAVCRVHKSYMVAVDKIESIEKDRIKIGNERIPISDTHKSH
                     FFSIIGRK"
     gene            8207..11158
                     /locus_tag="PLB46_02025"
     CDS             8207..11158
                     /locus_tag="PLB46_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRF75318.1"
                     /translation="MKNVYLLVRSAFVLVCMALPAFVWGQAATLSGTITDPLGETLPG
                     VTVLIDGTGQGTISDIDGVYKLAFDAPGTYVITYSYIGFKKVTSTVTLASGQNMKQDL
                     ALELDALMLETAVVVGYGTVKSKDLTGSITNVGVKDFQSGNVTTPEQLIVGKVSGVQI
                     TSNSGMPGAGSRIRIRGGTSLNASNDPLIVVDGVPVDNNGISGSGNALSLINPDDIEN
                     ITILKDASASAIYGSRAANGVIIITTKKGFSDTKLHVDVSTTNSFATVTQTVDVLSAD
                     ELREVITANGTATQLALLGDENTDWQNELYRLGITTDNDITFSGGIKNFPYRLNLEYM
                     MEQGVLDRSQLNRMGSSLSINPTFLNGKLKTDANGKFYHTDNFFADQGAIGSAITFDP
                     TKPVYDDASVYGGYYEWQNAAGLIGLAPKNPVGLLNQRDDESDVNRFIGNLKLDLELF
                     KDFHGVVNGGLDMSRSAGTIVVPAEAASSYSNGGVNNQYEQSKDNRLLEAYFNYKVDA
                     SKIKSKFDFTGGYSYQFWSRKEPAFASLSFDGDTITPAGIPTETENALISFYGRLNYT
                     LNNKYLLTATLREDGSSRFSPDNRWGLFPSVAFAWLVSDEAFMDNSTMYLKFRAGYGV
                     TGQQDIFNDYPYIANYDSSTSTAQYQFGDVFYYLLRPDGYDPNIKWEETSSYNVGIDF
                     GFAGDKVSGSLDVYKKVTEDLLATIPIPAGTNFTNQILTNVGSMENTGIEANINVVAI
                     NKKDMNLEFGINGTANRNEITKLTKVEDPNSPGILVGGIEGGIGNNIQIQSVGYAANT
                     FYSYEQIYDEDGNPIEGEYVDQNNDSIINADDLIQGESPTPDFYAGFYSSFRYKKWSV
                     GFSLRGEFGKYMYNNIASTRGIYQNIPSGSYMQNLSADYLESEFQTYQLFSDYYIQNA
                     SFVRMDNLSVGYDVGSVFNDNASLMISAIVQNVFVITPYTGLDPEIATGIDRSIYPRP
                     RIYSISANLKL"
     gene            11170..12759
                     /locus_tag="PLB46_02030"
     CDS             11170..12759
                     /locus_tag="PLB46_02030"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF75319.1"
                     /translation="MKNKIILPVMLLALVSITTPSCFNDLDLEPAYGLNSVAVYEDPA
                     NYIHVLAKIYSGLAISGNQGPAGNPDISGIDEGFSQYVRVLWNLQEVTTDGAKCAWND
                     PGIPELNKMQWSSTNDFVEAMYNRIYFQLPLCNEFLREASDEKMSDRGFSEADQNLIR
                     GYRNEVRFLRALSYFHAMDLFGNVPFITEESVFGSDAPERITRGDLFAYVESELLAIE
                     SALPATNEYGRATKGAVQTLLAKIYLNAEVYTGTPRYNDCAEYCSKVINEGGYSLEPV
                     YDHLFMADNHKSNEIIFPVTFDGLFTQTYGGTTFLTHSTIGGDPMSALDSSYFGVQFG
                     WGGNRATPEFAANFPDNDDARNMFFTEGQTLEFSDDTQLGQFIVGWPIAKWRNITSTG
                     VLGSDATGNFVDTDFPMFRLADVYLMYAECAARGAGDVSLGLGYVNAIRERAYGDAAH
                     NVASITPESVLAERARELQFEAQRRTDLIRFGLFTGGEYVWAFKGGPYNGTSVPDHLN
                     LFPIPADDIIANPNLIQNPGY"
     gene            12847..14682
                     /locus_tag="PLB46_02035"
     CDS             12847..14682
                     /locus_tag="PLB46_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRF75320.1"
                     /translation="MKKLLLILCLCISGQIFAQQIQQICPPNWWIGMRDSSVQLMIHG
                     NNIGAHHLKINYPGVTLVSATSVENNNYLFAEIVIDEKAAPGTINLEFSSGKNTFTYP
                     YTLKAREKRNFNYGLDAGDFIYLLMPDRFSNGDLNNDIVAKMQEKVMDRNDPKGRHGG
                     DLQGIINHLDDIKELGVTATWCTPLITNDQPKYSYHGYAATNHYEIDPRYGNGDLYKM
                     YVDESHKRNMKVVMDIVHNHVGSEHWFIKDLPMKDWIHQWDTGFVRSNYRTSVKLDPY
                     ASEYDQKKMNEGWFDQHMPDLNQDNVFLSQYIIQNNIWWIENYHIDGFRLDTYPYSDL
                     QFLTNWAKAINYEYPGFGFFGEVWVNGVGVQGYFHGNNHLNTGYNSLLPGLTDFNLYD
                     ALHTGLNENFGWYEGLARIYHNLSQDYMYGDVMKNVLFLSNHDVSRFYSMMSENVDKF
                     KMATTFLMTTRGMVQWYYGDEILMKNYVSPEDGKVREDYPGGWPSDGINKFDFANLNA
                     NEKDFYNYVSTLANWRKNAPAISEGKLMQFIPENGVYVYFRYTDNQVVMVVMNTGKNN
                     YSLDLKRFAEMTNGKTTATNVITGVSSNLNSLTVGTMEANVYELK"
     gene            14811..>17119
                     /locus_tag="PLB46_02040"
     CDS             14811..>17119
                     /locus_tag="PLB46_02040"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75321.1"
                     /translation="MKKILLALTLIIGSTTLFAQTSKKTTTTAPVITTIPKSLFDGLH
                     WRNIGPFRAGRCIAVTGVYNNNQVYYAGAVGGGLWKTTDGGVDWHCVSDSTFGSSSVG
                     AVEVAQTNPNVVYVGMGEVEMRGNISFGDGMYKSTDAGKTWKHIGLKETYAIGSIAIH
                     PQNENIVYVAAQGHNWGPNKERGLYKTTDGGATWNLILKGYDDTTGCADVKIDPTNPN
                     IIYASMWKAFRTPYSLSSGGKGSGLYKSTDAGATWKLISENPGMPKGLMGKIIVTIST
                     VNNQNLWAAVENIDNAGIYSSNDGGETWNLNTQENDLIQRPWYFSNLYVDPQNENELY
                     ILSVEYWRSFDGGHEFSRAETNTWDNHVMWINPANPNNYIIGGDGGPTITFNDGLTWS
                     DIDIPTAQFYHVNIDNEFPYNVYGGQQDNSSIKIKSRTGDGDIGEDDWRSVAGGEAGF
                     IVPDPRDARITYGGEYDGIFSSYNEYTDMYRIISVNPEQHYGAGANTAKMRFNWTFPI
                     IFSPNDKTCLYATSNYVHRSYDGGVSWETISPDLTRNDPKTLKESGGPVTLDNTGVEN
                     YATIFSLQESPVMAGVIWTGSDDGLVYLSNDNGKTWNNVTPANLPEWSMISYVEPSHF
                     DAATCYISATRYKLDDMKPYIYKTNDYGKTWTKITDGLPENMYNRCVRNDPNKKGVLF
                     AGLETGVWFSVNDGASWQSLKLNMPVTPIVDIQIHKGERDVVVASHGRSFWILDDITP
                     IYQLAEDWKNINNKPAHLFPVHEGWRTPGP"
BASE COUNT         5355 a         2864 c         3319 g         5581 t
ORIGIN      
        1 ggctgttttg ccaacttgcc atcttcagta ttgccaccgc ctagctcttt tgccattttt
       61 tcgagggcct cgcgaatcat ggcttgtttt gcgccatttc agctaactct ttactcatgc
      121 cgctttggcc gcctttttgc tgactttgag ggttttgacc cttcatttta ttgagttgct
      181 cgttgagttg tttttgcatt tcactcatcg acggcatttg tgattcgttt ttacctcccg
      241 gtttctgaca ttgctgcgag cccggcattg actgagccat ttgctgttgc atttgttgta
      301 acacctcatc caacatcaag gccaggttgt tataaccggt catggtttgt tgctgataca
      361 aattgctctg attaatttga cgttcaccca tttggtccac cgaattttgc agattggtat
      421 ttacatcact catttcgcgg gtaatgaaac tgctgatttg agtaacacgt ttagccagcg
      481 ccaataaact atcttctaca attttcgtgt cttcttttat tttttgctgt tgcgccatca
      541 aatcaatgaa cttaggattg ctcgatttga gggtttttac ttctttcatt aacgattcct
      601 gatcaacgga caacttaatc aggttgtcga gcaattgttt taaagcttcg aggtcttctt
      661 cctgctgctg ctgctgttgt tgctgcattt gctgttgcat ttgcgcagcc atttctttca
      721 tttgctgagc ggcgcttttt tgttcttgtg cagcctgctt tttgttattt tgctgcattt
      781 gctgcttcga ctgttccatt ttttggtcga tttgctgctc ttgctgctca gtattaccta
      841 attcttttgg cgattcaagc tctttattca tttcccgcat ttcctgcatg tccttttgaa
      901 catcatcgaa atctttattc agctcctctt gtttttcaag attttcttct tgtttgcttt
      961 gtttgttttc agtttgttct gcgagttttt gttgctcctc agaaagttga ttgagtttgt
     1021 cgatggtttc ctgcattttt tgctccatct ccatttgttt gaataattct aacatgcgat
     1081 cgagttcctg ttcgagttgc ttattgttca tttgcatctc tttcatttct tcgagcgact
     1141 gctctttatt cattttttcc attaattgct gaagttcttc catgagtttt ttcatttcct
     1201 caggaattac ttcatcaaac attttttcta actgttcttg tttttctaat atttcaggat
     1261 cgggttgcaa atattctttt tgtaactcga ggttttcttt atactgttct tttatttctt
     1321 ctaccgattg ttgaatgttt tgattttttt gcatcaaatt ttcaatcgct tttttatctt
     1381 cccagtttaa ttccttttta tcaaaaattt tatcttctaa tttttcagtt tgttttttta
     1441 catcagcaat atcatccaac acttcttcca gcttatcttt tatatcttca tttttttcat
     1501 ctttaatatc ttgtaattcg tcgagtgttg gcactacaaa ttgcatagct gcggtgcgtg
     1561 tagatttact gccgtgtaca ccatcattat cccaaacctc aaaaaagtaa gtaaccctat
     1621 cgcccggtgc aagtgtttta gcgcgtaagt cgaatgaatg ggtatagcga ttgcttttta
     1681 caccggaagg gacagataat acgtctttat tttcttcttt tacaaaataa ttgtctgcag
     1741 catctcttcc ttctacttta tatttaaagt acatggattt aaaaccgtag tcatcattta
     1801 tttctccaag aaaatataaa aatttattgt ctgtgctgtc tttaaattgt tcagcagtta
     1861 tttgtggaaa ctcatccgga attaccgtaa tagtataact aattgtatcg cggttatata
     1921 cttgtgtatt gccaatgcca attttatata cttcattttg catggcttta cgtgatgatg
     1981 taaaagacga cttaccatct ttaacggcat tcattttttg gtcgttaaaa aacaactcaa
     2041 ttacatctgt attttcagtg ttaaacaacc actcaagatt ggtgccttcc ggaactaata
     2101 agtcgccggt gttttgtaag cgttcatctt taatgccggt ataatccgga taattaacat
     2161 tgatatcgaa atttaataat gttggtttag gcactacggt taatgtgcgt tctttagatt
     2221 gatagccatt tgcttcaaaa tgaaaatctg tatttgcagg cactttattg aagacataag
     2281 taaatgcatc atcatctgat ttttgcattg tataggttcc ggttgcggtt actatggcaa
     2341 cctgttccgg tagaatatct ccggtaattt gaagttttag ttcgaaatct tcgtattgca
     2401 caacttccaa cgatttgttc tgtaccagga agttgaacgg tgctgctttt tcaaaaaaag
     2461 tattgttttt taataatcgt aagttactgt ctttgagcac attaggtgcg gcgaagatga
     2521 caaaaaccag aacgcccaac ggcggaagtg catatttgag atacttttta tttttattta
     2581 aatcgatagc cagcgaaaat ggaatcggtt taattttttc gattttttga ttgataccgg
     2641 cttcaataag tagttgattg ccagtgcctt ctgcctgttc gcgtagctcc aatatgttta
     2701 ataacttatc ctgaatttca gaaaagtgtg ttccgataat acttgcagcc gttttatgat
     2761 taatggtttt gcccaagcga ttatatttga gcaaaggggt gataatatta ttccataaca
     2821 aaaaacccgc tccacctaaa aagctgtaaa acagcacttt gcggacagtg gttgaaaaat
     2881 aaaaacggta ttccagaaaa ctgaaaagta aataagcaga aaccaatatt gccgccgtaa
     2941 acaatactcc gcgaatgagt tgattcaggt aaaatttcct gataaactca tcgagttttt
     3001 gaatgagaac ctgataatta ctattgcttt gcatacttat agaacaatat taacgcaaat
     3061 tgagccaaaa ttgtaccatg ataacgaaat ttaacctgta aagttgcaca tactcggttg
     3121 aggctggtat attctttctc ttaccattat tgtatcgctt ctttagccgt ttgaacttgc
     3181 ttctgtttac tttccttaac tttgccccat ggatgcttta acccgcgagg agttaccgat
     3241 tgattttgat ccgaggtgga tagcagtatt aaagcaaatg aaggaacaat ttgggaaaaa
     3301 accggaacta gaagctgttt tattccttat tggaatgaat gaattaggtc gtgtaattga
     3361 aaaattcacc aaagaagaaa agcaggattt aatgcatata gcagtttgta aattgctgag
     3421 ctacgatgga ttttatcgat ttgcaggatg ggatgatgac aattggccaa tgtgggaagc
     3481 agcagaaaaa ttgccacaat ggacaaacga agatcaggaa agagcaatta aattaaacat
     3541 catcagatat tttgaagaaa acaacatcat atgaaaaaaa caacattaat tgcagtatgc
     3601 ctgatggcat ttgtggcgtg taaagaaaaa acaacgccga cacctgaaga gccaaaagca
     3661 gaaattcaaa caacaacaca aacaaataca atggaaaatc agaacgacac aacgcagtat
     3721 tacgtaaaca tcagcacatc ttttggtgac atggtagtga agttatacaa cgaaacacct
     3781 aaacaccgcg acaattttat taagttggta aaagacggtt attataatga tttgttattt
     3841 catagagtta taaaagattt tatgattcag ggtggtgatc cggcttctaa aaatgcagga
     3901 cccaatgtac aattaggtgc aggcggaccg ggttatacta ttccagcaga atttaaccgc
     3961 aactttattc ataaaaaagg agcacttagt gcagctcgtc agggcgatca ggttaatcca
     4021 accaaagcaa gttcaggctc acaattttat attgttcagg gaaaagtaac agacgcagct
     4081 cagttagaac aaatgggtcg tcaaacaggc aatagttata ctccggaaca aacagctatt
     4141 tatagttcta ttggtggtac accattttta gacaatcaat acacggtgtt tggtgaagta
     4201 attaaaggat tggaagtaat agataaaatt gctgcagtta aaacagcata tggcgacaga
     4261 ccacaacaag atgttaaaat gaccatgaca atggttacga tgtaatttta cattaacaaa
     4321 atatataagc ctgtagggta tacattacct tacaggcttt tttttgcagg atgttttgcc
     4381 cgttttgcag acaaagtggt taaaatgaca aaacaaattt gagactggtg gtgggagagg
     4441 atacttttgt tgcatacatt aaataaatag tatgtattcc atcacaacaa ataacctaaa
     4501 ttatcgcttt aatgggaagg aaaccgtttt aagtgatatc aatttacagg tcccaaccgg
     4561 ttcaatatat gggtttttgg gaccaaatgg cgcaggaaaa actaccacac tgcgtttaat
     4621 ccttggcttg ttgaaaaagc aggatggcga aatcaatatt tttaatcagc catttgaaaa
     4681 aaacagaatt gaaatcctcc ggaaaaccgg ttcattaatt gaatcgccat cattgtatgc
     4741 ccatttatct gcaaaagaaa atttggccat tcttcaaaaa gtatatcaat gcaaaccttc
     4801 cagaattgac gaggtattaa aaattgttgg tcttgaaaat accggaaaaa agagtactgg
     4861 taaattttct ttaggcatga aacaacgctt gagtattgcc attgcattaa tgcacgaacc
     4921 agaattatta attctggatg aacccacaaa tggccttgac cccaacggca taattgaaat
     4981 gcgcgagttg cttaaacaac tcaataaaga ttttgggatg acaatactgg tttctagtca
     5041 cctgttggct gaaatagaaa aaatggtgac ccatgtgggc attattaata aagggaaact
     5101 cattttccag ggcaccatgg gcgaactcca cgaagcgcag catcaacagt catttatttg
     5161 tttcgaaaca ggaaatattg aaaaaaccag agcagtgctc aacgccaacg gaattcaatt
     5221 tcaacaagaa ggaagtcaat tgcatgtgcc tgttttagaa cgcgaattaa ttgcatctat
     5281 aaacaaacaa cttgttgagc aggatataaa agtatatcaa attagtttga ttaaaaatga
     5341 tttagaatca atatttattg acctggtaaa ataaattata tgcaacttat aaatagtata
     5401 aaaagcgaat ggttaaaaac cagaaaaagt gctgcatcat ggctttgttt aattggcgga
     5461 ttatttattc cactaatttt tcttatcgga tttttataca aaggcgaaac actgagcaca
     5521 tatggtgatg cagaaaatgt ttggacagca catttttttc aggtatggca gtcgatggta
     5581 gtatttttat tgcctatggg acttattttg gcgacaagtt taattaccca aatggaattt
     5641 agaaataaca cctggaaaca attgcacact acaccacaac ggtattatgt aatattttgg
     5701 gcgaaattta ttgtgataat tggcatgaca ctgcagttct ttttttactt taatctggga
     5761 tttattttgt cgggaattat tccatcgtta gttgtagata aacaattacc ggatgttata
     5821 tttccataca gccaattttt tacgagtaac tggaaaatat ttgtcacttg tttaccggta
     5881 ttagcattac agtttttaat tagtttgcga tttaaaaact ttttagtgcc aattggcgta
     5941 ggcttgatgt tattagtagc tacattaata ctggtaggca attgggatca tgtgtattta
     6001 agtccgttca gttacagtcc cctaaccatg atgggcacac ccgaaactat gaaaaatatt
     6061 aatatttaca attggagttg ttattatttt atcgccttta tggtaattag ttacctgatg
     6121 tatattagaa aaactgaaaa agggtaagca gaataatttg tttaatttta atcgcatata
     6181 aaaatgaaaa aaccggttgt catattatta catgttggat actggctggt atttttattg
     6241 ctgttatttg ttttgtatgg attaagcaat gctgcagcac taagtaatga gcaagacccg
     6301 ggtgttggtg caggcgagtg gtttaaactc atgttttcaa ctactatttt gccgggtgtt
     6361 attagttttt atacttttta tttcatcatc ttttccaggt ttttacagaa aagaaaaatt
     6421 cctgcatttt ttattagtgt ttttgttgcc tcttatgttg cagcaataat tggaggtgct
     6481 gttggctcta ttaattattt tttaggctat cattttttaa tggataaaaa cttgccaaca
     6541 gccttgtcta tgcttacttt tttagcattt atcgcattgc taaatggtat tattgggtta
     6601 gtaatgagag gttttattac ctggtattcc gaaataaaaa tgaaagaaga tttgaataaa
     6661 aagaattttg aaatggaact ggctttagtt aaatcgcaga ttaatcctca tttccttttt
     6721 aatacaatta ataatattga tgtactcatt acaaaagaac cggaattagc ctctaaatat
     6781 ttgaatacgt tatcagacat tatgcgtttc atgttgtatg aaacaaaagc tgaacgaata
     6841 ttattatcaa aagaattggc atacatagaa aaatttattg gtttacataa aatcagaagc
     6901 aataatccgg actatgtaca ctttaccgtc aacggagctg cacctaatat gatgattgct
     6961 cctatgttgt ttattccatt tatcgaaaat gcctttaaac atgttgcgga taaaaaggca
     7021 aaccctgcaa ttgttattag cttgaatatt gataaagata aaatcacttt catttgtcaa
     7081 aacaatttta aggaagaaac ccgcgatgaa aatgaacata gtggacttgg tgacggatta
     7141 atcagaaaac gattgcagtt attgtatcca gataaacatg aattaacagt taccacgcaa
     7201 aacaatattt atgctgtata tttaaccatt agtagttgag agatgaaatg tattattgta
     7261 gaagatgaac cattagcgct tgagcgcaca aaagggtatg ttgaaaaact gcctttttta
     7321 aaattgttgg caacgttcga caatggtgtt gatgcattat tgtttttaaa agataatacc
     7381 attgatttac tgtttttgga tatcaactta ggtgaacttt ctggcatcca aatgttggaa
     7441 gccgctaaac cggattgtgc tgtaattatt acaacggcct accacgaata tgcattaaaa
     7501 ggatatgaat tgaatgtaac cgattattta ttaaaaccgt ttacattcga acgttttttt
     7561 caagctgttg aaaaggcaaa agtttcactg gataaaaagg aagtccctga aaaaaaatct
     7621 gtatttataa aaacggagta ccgcttagaa caagtactct taaccgatat tatttacatt
     7681 gaaggtatgc gcgattatcg tcgcatattt ttacatggca aatcgattat gactttacaa
     7741 acatttaagg agctcgaaga aatgatacct ccggctgcag tttgccgcgt tcataaatcc
     7801 tatatggtag ctgtcgacaa aatagagagt attgaaaaag acagaataaa aattggcaac
     7861 gaaagaatac ctatctcaga cacgcataaa agccactttt tcagtattat aggtagaaaa
     7921 taaccttcaa attgcgtgca aaatggcact ttttgtcaaa acttcaataa gtagggtgtt
     7981 aaatttttga tgacaattta agccttcagc taacaataat atcttccatc tgttcatttt
     8041 tttgataaac atcaaaaaaa ctgttacaaa atccttacaa aaagacttat tttacggttt
     8101 acaattagta aaattgcagt gtatttcact tattacacct acttaacatc agttgtatgt
     8161 ctgaaatgat gtttgatatt attttaacaa ccaaatctaa aagttcatga agaatgttta
     8221 tttactagtc agaagtgcat ttgttctggt ttgtatggcg ctcccagcct ttgtttgggg
     8281 tcaggcggct actttgtctg gaactatcac cgatcctctt ggagaaacac taccgggtgt
     8341 taccgtttta attgacggca ccggtcaggg aacaatttcc gacattgatg gcgtttacaa
     8401 gctcgcattt gatgccccgg gaacttatgt aattacatat agttatattg gatttaaaaa
     8461 ggttacctca acagttacac ttgcttcagg tcaaaatatg aagcaggacc ttgcactaga
     8521 acttgatgca ttgatgctcg aaacagcggt tgtggttggt tatggtacgg ttaagtcgaa
     8581 agacctcacc ggttctatta caaacgttgg cgttaaagat tttcaatccg gtaacgttac
     8641 cactccggaa caattgattg taggtaaagt ttccggcgtt caaattacct caaacagcgg
     8701 tatgcctgga gctggaagcc gtattcgaat cagaggcggt acttcactta atgctagtaa
     8761 cgaccctttg atagttgttg atggtgtgcc tgtagataat aatggtattt ccggttcagg
     8821 aaatgcgttg agtttgatta accctgatga tatcgaaaat attaccattc taaaagatgc
     8881 atcagcatct gcgatttacg gttcacgtgc agcgaatggc gttattataa ttactactaa
     8941 aaaaggcttt tcagacacca aactgcatgt tgatgtatct acaactaatt catttgcaac
     9001 tgttacacaa acagttgacg tgctttctgc agatgaattg agagaagtaa ttactgcaaa
     9061 cggaacagct acacaattag ctttattggg agatgaaaat accgattggc aaaatgaatt
     9121 atatcgcctt ggtattacta cagataatga tatcacattt tcaggtggta ttaaaaactt
     9181 cccgtaccgt ttgaatttag aatatatgat ggaacaaggg gtgttagacc gttcacaatt
     9241 aaacaggatg ggttcttcac tttcaattaa tccaacattt ttaaatggta aattaaaaac
     9301 agatgccaat ggtaaatttt atcataccga caactttttt gccgatcagg gtgcaatagg
     9361 ttctgcaatc acatttgacc caaccaaacc ggtttatgat gatgcttccg tttatggtgg
     9421 atattacgaa tggcaaaacg ctgccggctt aattggtctt gcgccaaaaa acccggtagg
     9481 tttattaaat cagcgtgatg atgaaagtga tgtaaaccgt ttcatcggta acttaaaatt
     9541 agacctcgaa ttatttaaag attttcatgg tgttgttaat ggtggtcttg atatgtcgcg
     9601 aagcgcagga acaattgttg ttccggcaga agcggcgtcc agctattcta atggtggtgt
     9661 aaacaatcaa tacgaacaaa gtaaagacaa ccgtttatta gaagcatact ttaattataa
     9721 agttgatgca agtaaaataa aaagcaaatt cgattttacc ggtggttatt cttaccaatt
     9781 ctggtctaga aaagaacctg catttgcaag tttaagtttt gatggtgata ccattacacc
     9841 tgcaggtatt cctactgaaa ctgaaaatgc attgatttca ttttatggtc gtttgaatta
     9901 tactttaaac aataaatatt tattaactgc cactttacgt gaagatggtt catcaagatt
     9961 tagtcctgac aaccgttggg gtttattccc ttcagtagca tttgcatggt tggtaagtga
    10021 tgaagcgttt atggataatt ctaccatgta tttaaaattc agagccggat atggtgttac
    10081 cggacaacag gatattttta atgattatcc atacattgcg aattacgatt caagtacttc
    10141 tacagcacaa tatcagtttg gagatgtatt ttattattta cttcgtccgg atggctacga
    10201 cccaaatatt aaatgggaag aaacttcaag ttataacgta ggtatcgact tcggttttgc
    10261 cggcgataaa gtttcaggta gtttagatgt gtataaaaaa gtaactgaag atttattagc
    10321 aactattcca attcctgccg gaacaaactt cactaaccag attttaacca atgtgggaag
    10381 tatggaaaat accggtatag aagcaaatat taatgttgtg gccattaata aaaaagatat
    10441 gaatcttgaa ttcggaatta atggtactgc aaacagaaat gaaattacca aattaacaaa
    10501 agtggaagac ccgaattctc caggtatttt agttggaggt attgaaggtg gtattggtaa
    10561 caatattcaa attcaaagtg tgggatatgc tgcaaataca ttttattcat atgagcagat
    10621 ttatgacgaa gatggcaatc cgattgaagg cgaatatgta gaccaaaata acgactctat
    10681 tattaatgcc gacgatttaa ttcagggaga aagcccaaca ccggatttct atgcaggatt
    10741 ttattctagt ttccgttata aaaaatggag cgtaggattt tcattacgcg gtgaatttgg
    10801 aaaatatatg tataataaca ttgcgtctac ccgcggtatt tatcaaaata ttccgtctgg
    10861 tagttatatg caaaacttat cagcggatta tttggaatct gaattccaga cttatcagct
    10921 tttttctgat tattatattc agaatgcttc attcgttaga atggataatt taagcgtagg
    10981 ttatgatgtt ggtagtgtat ttaatgacaa tgcttcttta atgatttctg caatcgttca
    11041 aaatgtattt gttattacac cttacacagg attagatccg gaaattgcaa caggtatcga
    11101 ccgttcaatt taccctcgtc cacgtattta ttcaattagt gctaatctta aattgtaaat
    11161 taatcagcga tgaaaaataa aataatttta ccggtaatgc tgctcgcatt agtaagtatt
    11221 accactccgt cgtgttttaa tgatttggat ttggaaccag cctacggttt aaactctgtg
    11281 gcggtgtatg aagatcctgc aaactatatt cacgtactgg caaaaatata ttccggttta
    11341 gcaatttccg gtaaccaggg tcctgcggga aatcctgata tttccggcat cgacgaagga
    11401 ttttcacaat atgttcgcgt attgtggaac ttacaggaag ttaccaccga tggcgcaaaa
    11461 tgtgcatgga acgacccggg tattcctgaa ttaaataaaa tgcaatggtc atcaactaac
    11521 gattttgttg aggctatgta taaccgtatt tatttccagt tgcctttatg taatgaattt
    11581 ttacgcgagg cttctgatga aaaaatgagc gaccgcggtt ttagcgaagc tgatcaaaat
    11641 ttaattcgcg gctatagaaa cgaagtgcgc tttttacgtg cattgagtta tttccatgcc
    11701 atggatttat ttggtaacgt gccatttatt accgaagaaa gtgtttttgg ttctgatgca
    11761 cctgagcgta ttacaagagg cgatttattt gcttatgtag agtctgagtt attagcaata
    11821 gaatctgcac ttcctgcaac taatgaatat ggcagagcaa ctaaaggtgc tgtgcaaact
    11881 ttattagcaa aaatttattt aaatgctgaa gtatatacag gaactcctcg ttataacgat
    11941 tgtgctgaat attgctccaa agtaattaat gaaggaggat attctctcga accggtttat
    12001 gatcatttat ttatggcgga taaccacaaa tcaaatgaaa ttattttccc tgttactttc
    12061 gatggtttat ttacgcaaac ttatggtgga acaacatttt taactcactc aactattggt
    12121 ggtgacccaa tgagtgcttt ggattcatct tattttggtg ttcaatttgg ttggggagga
    12181 aatcgcgcaa ctcctgaatt tgcagcaaat ttccctgata atgatgatgc gagaaatatg
    12241 ttttttactg aaggacaaac tttagaattt tcagatgata cccagctggg tcagtttatt
    12301 gtaggttggc caattgcaaa atggagaaat ataacaagca caggtgtgct tgggtcagat
    12361 gccacaggta actttgttga tactgatttc ccaatgtttc gtttggcaga tgtatatctg
    12421 atgtatgcag aatgtgctgc tcgcggcgca ggtgatgtat cacttggttt aggttatgta
    12481 aatgctattc gcgaacgtgc ttatggtgat gccgcacaca atgttgcatc tattacacct
    12541 gagtctgttt tagcggaaag agcacgtgaa ttacaatttg aagctcaacg cagaaccgac
    12601 ttaatacgat ttggtttatt cacaggtggc gaatatgttt gggcgtttaa aggtggccca
    12661 tacaatggaa catctgttcc agaccactta aatttatttc caattcctgc tgatgatatt
    12721 attgctaacc ctaatttgat acaaaatccg ggttattaat tcattattcc ttattttaaa
    12781 agaggctttc cttaaacgaa agcctctttt tttatgttta accaatacat taattatttt
    12841 tatacgatga aaaagttatt gctcatttta tgtctctgta tctccggtca aatttttgca
    12901 caacaaattc aacaaatttg tccgccaaat tggtggattg gaatgagaga ctcttccgta
    12961 caattaatga ttcatggcaa caatattggt gcgcatcatt tgaaaattaa ttatcctggt
    13021 gtaacattag ttagtgcaac atcagtagaa aataacaatt atttatttgc tgaaattgtg
    13081 attgatgaaa aagcagcacc cggtacaatt aatcttgaat tctcttccgg aaaaaataca
    13141 tttacttatc cgtatacatt aaaagcacgc gaaaaacgca attttaatta cggcttagat
    13201 gctggtgatt ttatttattt gttaatgccc gaccgttttt caaatggcga tttgaataat
    13261 gatattgttg ccaaaatgca ggaaaaagta atggacagaa acgaccctaa aggcaggcat
    13321 ggtggcgatt tacagggtat catcaatcac ctggatgata ttaaagaact tggtgttaca
    13381 gcaacatggt gcacgccatt aatcaccaac gaccagccga aatatagtta ccatggatat
    13441 gctgcaacta atcactatga aattgacccg cgttatggta acggcgactt atataaaatg
    13501 tatgttgatg aatcacacaa acgcaatatg aaagtggtga tggacattgt acataatcat
    13561 gttggttcag agcattggtt tattaaagat ttaccaatga aagattggat tcatcaatgg
    13621 gataccggat ttgtaagaag taattatcgc acaagcgtaa aacttgaccc atatgcaagt
    13681 gagtacgacc agaaaaaaat gaacgaagga tggtttgacc aacatatgcc tgaccttaat
    13741 caggataatg tttttttaag tcagtacatc attcaaaata atatttggtg gatagaaaat
    13801 tatcatattg atggatttag attagataca tatccgtatt cagatttaca atttttaact
    13861 aattgggcta aagcaataaa ctatgaatat ccgggatttg gtttttttgg cgaagtatgg
    13921 gtaaacggtg ttggtgttca gggatatttt catggaaata atcatttaaa taccggctac
    13981 aactcactgc tgccggggct tacggacttt aatttatatg atgcattaca cacgggatta
    14041 aatgaaaatt ttggttggta cgaaggcctt gcccgcatat atcataatct gtcgcaggat
    14101 tatatgtacg gcgatgtaat gaaaaatgtt ttgtttttga gtaatcatga tgtaagtcgt
    14161 ttttattcta tgatgagtga aaatgtagat aaatttaaaa tggctactac atttttaatg
    14221 acaacaagag gaatggtgca atggtattat ggtgatgaaa ttttaatgaa aaattatgtt
    14281 tctccggaag atggaaaagt acgcgaggac tatcccggtg gctggccttc tgatggtatc
    14341 aataaattcg attttgctaa tctcaatgcc aacgaaaaag atttttataa ttatgtaagc
    14401 acattggcta attggagaaa aaatgcaccg gcaataagcg agggaaaact catgcaattt
    14461 attccagaaa atggtgtgta tgtttatttt agatataccg acaatcaggt ggtaatggta
    14521 gtaatgaata ccggaaaaaa taattacagt ttagacctga aaagatttgc cgaaatgaca
    14581 aacggaaaaa caacggctac aaatgttatt acaggtgttt cgtccaactt aaatagtctt
    14641 accgttggca ctatggaagc aaatgtttac gaattaaaat aatgatataa accctgtttt
    14701 gattcttaaa aaaggatgaa gcagggttta tttgtttaat tgtaattatt aaatcttaac
    14761 aattccccaa ttcctcaaat gtttatattt gtgataacga ttcacaaatt atgaaaaaaa
    14821 tactgctcgc acttaccctt attattgggt caaccaccct ttttgctcaa acatcaaaaa
    14881 aaaccacaac tacagcacct gttattacta ccattccgaa atcgttattt gacggtttgc
    14941 attggcgtaa tattggacct ttcagggctg gaagatgtat tgcagttacc ggagtgtaca
    15001 acaataacca ggtgtattat gccggtgctg ttggtggagg cctttggaaa actacagatg
    15061 gcggtgtaga ctggcattgc gttagcgata gcacatttgg ctcttcaagt gttggagctg
    15121 ttgaagtagc acaaacaaac cctaatgtag tatatgttgg gatgggtgaa gtggaaatga
    15181 gaggaaacat ctcttttggt gatggtatgt ataaatctac agatgccggt aaaacatgga
    15241 aacatattgg cttaaaagaa acctatgcta ttggctcaat tgccattcac ccacaaaatg
    15301 aaaatattgt ttacgttgca gcacaaggtc ataattgggg accgaataaa gaacgtggtt
    15361 tatataaaac aaccgatggg ggagcaacat ggaatttgat attaaaagga tatgacgata
    15421 caacgggttg tgccgatgta aaaattgacc cgactaatcc taatattatt tatgccagta
    15481 tgtggaaggc atttcgcacc ccatattcgt tgagtagtgg tggcaaaggc agtggattat
    15541 ataaaagtac cgatgcaggt gcaacatgga aattaatttc tgaaaatccc ggtatgccaa
    15601 aaggattaat gggaaaaatt attgttacca tttctacagt aaacaatcaa aacctatggg
    15661 ctgcagttga gaatatagat aatgccggta tttattcttc taacgatggc ggtgaaacct
    15721 ggaatttaaa tacacaggaa aatgatttaa ttcaacgccc atggtatttt agtaatttat
    15781 atgttgaccc gcaaaacgaa aatgaattat atattttaag tgtggaatat tggagaagtt
    15841 ttgatggcgg gcatgaattt tcacgagcag aaaccaatac ctgggataat cacgtaatgt
    15901 ggattaatcc tgccaatcca aataattata ttattggtgg tgatggtgga ccaacaatta
    15961 catttaacga cggattaacc tggagtgata ttgatattcc aacggcacaa ttttatcatg
    16021 taaatattga taatgaattt ccatacaatg tttatggcgg tcaacaggat aattcttcaa
    16081 taaaaataaa atcacgcaca ggtgatggtg atattggaga agacgactgg cgctctgttg
    16141 caggcggtga agccggattt attgttcccg acccgcgcga tgctcgtatt acgtatggcg
    16201 gagaatatga cggtattttt tcttcttata atgaatatac cgatatgtat cgaattattt
    16261 cagttaaccc ggaacaacat tacggcgcag gagcgaacac agcaaaaatg agatttaact
    16321 ggactttccc aataatattt tcacccaacg ataaaacatg tttatatgct acaagtaatt
    16381 atgtgcacag aagttatgat ggtggtgtaa gttgggaaac tatttctccc gatttaacaa
    16441 gaaacgaccc aaaaacatta aaagaaagtg gaggcccggt tactttagat aataccggcg
    16501 tagaaaatta tgctaccatt ttttcattac aagaatcgcc tgtaatggca ggtgtaattt
    16561 ggaccggaag cgatgatggt ttagtgtatt tatccaacga caatggcaaa acatggaata
    16621 atgttacgcc ggctaattta cctgagtgga gtatgattag ttacgtcgag ccatcacatt
    16681 tcgatgctgc tacttgttat atttctgcaa ccagatataa gttggacgac atgaaaccat
    16741 acatttataa aacaaatgat tatggcaaaa catggacgaa aattactgac ggcctgcctg
    16801 aaaatatgta taacagatgt gtgcgcaacg accctaataa aaaaggcgta ttatttgcag
    16861 gattagaaac aggggtttgg ttttcggtta atgatggcgc ttcatggcaa tcgttaaaat
    16921 taaatatgcc ggtaacgcct attgttgata ttcaaattca taaaggtgaa cgcgatgtag
    16981 ttgttgcatc acacggaaga agtttttgga tattagatga tattacacca atttatcaat
    17041 tggcagaaga ttggaaaaac atcaacaata aaccggcaca tttatttccg gtgcatgaag
    17101 gttggagaac gcccggccc
//
LOCUS       DAORKW010000023        16350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_38_length_16350_cov_5.050690, whole genome shotgun sequence.
ACCESSION   DAORKW010000023 DAORKW010000000
VERSION     DAORKW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16350
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38_length_16350_cov_5.050690"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLB46_02045"
     CDS             <1..226
                     /locus_tag="PLB46_02045"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRF75322.1"
                     /translation="RVGILTLFSVLTVPVEIDASNRAISWLQGSSLAGTMEMGKAKDA
                     LNWAGYTYVVAALGSLATLIYYIMLYMSRR"
     gene            378..818
                     /gene="rplM"
                     /locus_tag="PLB46_02050"
     CDS             378..818
                     /gene="rplM"
                     /locus_tag="PLB46_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRF75323.1"
                     /translation="MDTLSYKTRSANAATVTHDWWLIDAEGQTLGRLSAKVAAILRGK
                     NKPYFTPHTDCGDYIIIINAEKVKLTGKKMLEKEIDWYTGYPGGRKVANPKTVLAKQP
                     HKLLERSIKGMLPHNKLGSAMYKKLFVYAGTEHPHAAQKPKTLK"
     gene            826..1212
                     /gene="rpsI"
                     /locus_tag="PLB46_02055"
     CDS             826..1212
                     /gene="rpsI"
                     /locus_tag="PLB46_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRF75324.1"
                     /translation="METINAVGRRKNAVARVFLKKGKGTITVNGKDYKEYFAAKHLLH
                     NVTDPLETVNMANDFDVVVNATGGGIKGQAEAVKMGISRCLVKMNEELKPALKAKGFL
                     TRDSRMVERKKFGLHGARRATQFSKR"
     gene            1231..2109
                     /gene="rpsB"
                     /locus_tag="PLB46_02060"
     CDS             1231..2109
                     /gene="rpsB"
                     /locus_tag="PLB46_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRF75325.1"
                     /translation="MEKLSYNELLEAGVHFGHLRKKWNPKMLPYIFMEKKGIHIIDLY
                     KTLEKLDEAAAALRNIARSGKKIMFVATKKQAKETMEEQARRVGMPFATERWLGGMLT
                     NFTTIRKSIKKLQTIERMLSDGSAENMTKKERLVLSRERDKLEKVIGGVVQLNRLPAA
                     LLVVDIGHEHIAIAEAHRLNITTFGMVDTNCDPNKVDFAVPANDDAAKSIAIVIKYLT
                     DAIVEGLEERAKNKEEMEDARHDGKDEFGDKDEEIRLAGEDDADGQRKGPRGKGGPRT
                     ADRKPGARRPMTQRKK"
     gene            2196..3032
                     /gene="tsf"
                     /locus_tag="PLB46_02065"
     CDS             2196..3032
                     /gene="tsf"
                     /locus_tag="PLB46_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRF75326.1"
                     /translation="MSVTITAGDVNKLRQITGAGMMDCKKALGETGGDFEAAIDYLRK
                     KGQKVSEKRADRETNEGVVIAVTNQDGTFGTIMGLGCETDFVAKNQAYIDFAKSISDV
                     AMANKAESKDALLAMTLDGITIAEKIAEQVGKIGEKIDITDYALLSAETVVAYNHSNG
                     KVGVLVAMNKKGEAVETVGKDVTMQIAAMSPIAIDKNDVPEEVKTREMEIGREQARAE
                     GKPEAILEKIATGKLEAYFKDATLLNQKFVKDNSKSIRDVLTAVDKDLTVTAFKRIGL
                     GA"
     gene            3178..>4398
                     /locus_tag="PLB46_02070"
     CDS             3178..>4398
                     /locus_tag="PLB46_02070"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRF75327.1"
                     /translation="MLIRNCLIVLFFICSSAVVIAQQTDYVADEPAEQLLFVANKGQI
                     INEFGLPANDVLFSLTNNGATIFVYRDGLSYQFTKYHTGELQNVNAFLFDLEKDFPLQ
                     EQIVSTHRIDVKFVNANSSIEVIPESKSLLVENYFGEQYGNGITNIPTFEKITCKNIY
                     PNIDWVLYSNNGKLKYDLVLHPGANKNDITFQITGTDEAHLVDATHMQISSPLGEIND
                     KGLFCFEKESAEKINAAFILTNNYLKFDIDNYNPLHTLIIDPELDWSTFYGGFGEDAV
                     NGIDVDADGNIYITGYTSSSNAMAHLGYHNSYQGGFFDAYVAKLNPAGNRIWGTYFGG
                     NKGDYGTELVIDKNNKIYVTGFTFSNNFETTPGVHQEALGNNYDAFLFKLNDNGTLIW
                     STLFGGGSYDYARGI"
     assembly_gap    4399..4408
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4409..6182
                     /locus_tag="PLB46_02075"
     CDS             <4409..6182
                     /locus_tag="PLB46_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF75328.1"
                     /translation="KGAGNDEFLAKFSPSGLRIWATYMGGEADDYSRAVGVDLDNNVY
                     LVGYTESTTGIAFSGFDNTWSGNYDCTLTKYNSEGELIWSTYFGGNGDDNANGIDFDN
                     NNNIYIAIQTGTNSGLAHLGYRNSNAGGQDAMLAKFNPDGERLWSTYYGGTAEDMGKA
                     VAVSGEYVYLCGHTYSHSGIAAYAYQNTIGGNGDALLAKFDVFGNFYWATYAGGFWDE
                     FGRTIQILNDETIIFGGKTFSWDYPVTFGAHQIFYGGGTADGFIQRVYDCASAEIYYA
                     DADDDGYGDATVSELLCEPTTGFVANSNDCNDGNAFIYPGAIETCNSIDDDCDIIIDE
                     EVITAFITPMGPTVFCKGNTVLLQATLHPTYFYQWKKDGVNIVGATGGNYIVTKTGNY
                     TVDISTLGGCNAISNSIAVTVNNKPNPFIFAIGSTDICLTGNAKLKTVPFAGNTYQWY
                     KDGDIITGATSNMYMATAIGGYRVKETNAFGCFKNSNEITVTNTCKLENDISENSVLQ
                     VFPNQASNSVTILMSENLFNSENVQLVVYDNLGQKVWQQISPINNLNPMVECILPSQL
                     ANGMYIVELSANNQQYRTTLVVSR"
     gene            complement(6185..9154)
                     /locus_tag="PLB46_02080"
     CDS             complement(6185..9154)
                     /locus_tag="PLB46_02080"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HRF75329.1"
                     /translation="MKKYTLLLLFIYQLCQAQNLTQYVNPFIGTGGHGHTYPGATLPF
                     GFVQLSPDTRPDGYNDWDGCSGYHYSDSIIYGFSHTHLSGTGVADYCDILLMPTAGNV
                     ITTNIINNDPKQGYASAFSHKTEKAAPGYYSVMLEDEKIQVELTASTRVGMHQYTFPA
                     TNQANIILDLNHRDQLLEGSYIEIISPTKIQGLRRSSSWAKDQWLYFAIEFSQPFSIA
                     GLIDDNKTYKTAANNFKPIIISGTSLKGYVTFNTNNNNVITTKCALSPVSCAGAWKNM
                     AAEMPHWDFAKVKNEADYLWNFELSKIKVQSGSEAKKSLQAEDNKLVTFYTALYHTML
                     APNIYEDVDDQYRGRDNTIHVSKQFNNYTVFSLWDTFRALHPLMSIIDQDRTNDFIQT
                     FLAQYDAGGRLPVWELSGNETECMIGYHSVSVIADAAVKGITNYDLQKAYDAMKHSAN
                     LDHFGLSFYKAKGFIESNEEPESVSKTLEYSYNDWCIAQMGKLVNAPKSEIDYYTNRG
                     YNYLNVMENGFARPRFNGGWYSPYNPTEVNFNFTEANAWQYSLFYPQGNFGGELFEQR
                     LNELFTTNTGTTGRQQSDITGLIGQYAHGNEPSHNYAYLFNRTTTPWKTQYYVNEIMT
                     SFYHNAPDGLIGNEDCGQMSAWYVMSALGLYQVCPGKPEYDFGSAIFDTAVITLENNK
                     KVRIITKNKSDKTPFLKSVSWNKQLLPSLQIPHATLMQGGELVFEFTEQKINSEPLTD
                     SVPTLGLIAPVISGPGNSFTYKTTVEVSTQYDTGILKFSVNGSNQQYPAEKGVTAILN
                     ISSDAIITAQYCVINPDTKAETCSGVVTGKFKRRNNDYAIKYITAYDKQYAAGGSTSL
                     IDGLHGGADFRTGMWQGWQGTDMEVVLDCGSNQTFTKVGAGFLQDVRSWIWMPKQLEI
                     YTSTDGKAFSLLTTINNQVVTTNYDSKEIQKLTATVNATTARYIKFKAINFGTVPEWH
                     PGKGGQTWIFCDELIIE"
     gene            9378..10637
                     /locus_tag="PLB46_02085"
     CDS             9378..10637
                     /locus_tag="PLB46_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75330.1"
                     /translation="MKKSFTLCILVLTTFYFSYGQKDCSFDHTGKIPINDLAGGYFNG
                     VAGGLYPDGTNTRPPGHLAKCIEHVSQIQPLDVDGSPSPSGKIVMMGIGASNPMTEFN
                     KFKEISEYFEPLNDNLEIVNACVGGVAIQKMYEATDTYWLGVENLLEDAGLSALQVQV
                     IWVEQENTNSYDTAFNSATIGLVTDFLTLLRVFKDKFPNAQICYVSARAYAGFADPID
                     PEISHGLLYPRDYFNGWALKRLVENAINAEEDYDFDGPDAEIPLITWGTYHWTDGSTP
                     RMDGLFLDCEEDLSPDGLHLAGPGEQKIGTYMFDYFSTDTTAKYWFYDEANSGIINNP
                     VSKNTIQIYPNPVNGNTINFYAGTFDNYENIQYQICDAAGKVISTGIGYGKDNITIQL
                     QHIAPGAYYITAIGQQNIATGSFIVTQ"
     gene            complement(10640..11587)
                     /locus_tag="PLB46_02090"
     CDS             complement(10640..11587)
                     /locus_tag="PLB46_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRF75331.1"
                     /translation="MKEVVLGIDIGGTGIKFGLVDRDGNIVASDKAKTKGYPNPDMLP
                     ADLLKWSNEQCSALQLKTIGVGIGTPNGNYYKGTIDFAPNLPWKGVIPLKEYFEKTFS
                     LPVALTNDAKAAALGEMIFGAAKGMKDFIFITLGTGLGSGIVVNGNIAYGYDSLAGEV
                     GHTIVYRKGRECPCGRRGCLEQYVSAPGIVKTYYELVRHQNLDEFRTSIGNMIDSAEV
                     YKKAVEGDTIALEAFTKTGEILGFALANSVCYTRPEKIFLFGGLAQAGDLLFKPTIKS
                     FESYLLPIYKNKIPIVPSGLKESEAAILGSASLIWKELH"
     gene            complement(11613..12056)
                     /locus_tag="PLB46_02095"
     CDS             complement(11613..12056)
                     /locus_tag="PLB46_02095"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF75332.1"
                     /translation="MIQAILEVYNRHMQMTITDISAFTNEDDIWKTEEGINNSAGNLA
                     LHIVGNLNYFIGNILGKTGYVRDRDAEFSAKGIPQQELVNLLQDTNTMLSTTLSGLQD
                     DDLNKIYPIDKFGEGKTIGYVFVYLLAHLDYHLGQINYHRRLLNK"
     gene            complement(12062..13072)
                     /locus_tag="PLB46_02100"
     CDS             complement(12062..13072)
                     /locus_tag="PLB46_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRF75333.1"
                     /translation="MSNFPKRGNNPFRKTYLAIYLLIGAISIGIIGFILIEGYNFLDA
                     LYMTIITLGTVGFSEVNPLSPEGKIFTIFLIVLGLVIFGFFITQISRYFLDGEFIRYF
                     KIYKMQKEISQLKDHTIICGFGRNGREAARVLKQTNNQVVIIETEQSKLHTENADPVY
                     YFLEADATRDETLLSAGIMQASAIISTLPDDALNVFVCLTAKELNPNIKIISRATHVS
                     SIKKLKNAGAKNVIMPDKIGGAHMAMLVNNPDIEEFIDIMSTSTGDSFLIQEITSQKN
                     VVVGEIDTWKKTGATVIGIKSGENKYILNPGPDASIKVGDGVIIMGSKQQINNSLILF
                     KN"
     gene            13458..14225
                     /locus_tag="PLB46_02105"
     CDS             13458..14225
                     /locus_tag="PLB46_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRF75334.1"
                     /translation="MDFSIFLTADAWIALLTLTVLEIVLGIDNIIFISILSTKLPAEQ
                     QKKARRWGLGLAMGTRILLLLSISWILGLEADLFSIAGHGISGRDLVLLSGGLFLIYK
                     ATVEIHAKIEGDEHEDSPNIKKPSFTSVILQILMLDIVFSLDSVITAVGMVDADKVII
                     MILAVIIAVAIMLFAADPISNFVQKHPTVKMLALSFLVMIGVSLLIEGSGGHVEKGFI
                     YVAMGFSVVVEMLNLRMLSNAKKRKEKKNLEENEFGN"
     gene            complement(14280..15524)
                     /locus_tag="PLB46_02110"
     CDS             complement(14280..15524)
                     /locus_tag="PLB46_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRF75335.1"
                     /translation="MKLTLRIAWRYIFSKKSTNAINIISGISMSGIIIGSASLIIVLS
                     AFNGFENLVARLYSTFYPDITITPATGKVFAIDSNKLAKIQSIEGVEYISKTLEENVL
                     LAYNKQEHIATIKGVDTMYRYVTAVDDSVYYGDYILDYAYLNYNLDCAVMGSGVASTL
                     NVSLGLDYPAVQIFIPRRGVKSSSNPRNTFVQRGIQPMGVFRIQQEFDSKYVITSLQF
                     IQGLLDYQNGEISSLEIKTSSKNKVDDIMEEITAIMGDDYLVKNRFMQNEFLYRVMRT
                     EKWAVYFILTFIFIVAAFNMIGSIAMLVIDKKKDIGILRSLGATEASVRNIFFIQGVL
                     QTLVSIAVGFSLATILCYLQIQFGLVTIPGQGTFVVTAYPIALEWKDYVNVFLTVLII
                     GGIASYFPAYMAARQKWLFRNE"
     gene            complement(15521..15889)
                     /gene="rbfA"
                     /locus_tag="PLB46_02115"
     CDS             complement(15521..15889)
                     /gene="rbfA"
                     /locus_tag="PLB46_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRF75336.1"
                     /translation="MDSRRQKKIGQLIQDEMSTFLQKEGANYYGNKFVTVTDVSITSD
                     LQNCKIHVSVFDKNEGEKVVDSLNSNIRDIRRRFGHIMRNSLRIIPEMQFFLDDTLDT
                     VYRLEELFKKTKEDNNNPPA"
     gene            16027..>16350
                     /locus_tag="PLB46_02120"
     CDS             16027..>16350
                     /locus_tag="PLB46_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75337.1"
                     /translation="MFNLFYAFFNLFSILMVIPFLQIIFKMVTPPTSRPEFRWNIREM
                     LGILNYEFASYVNAHGAYSGLILVSIGVVFMFLFKNIFRYLALYFLAPLRTGVVRELR
                     QGVFDK"
BASE COUNT         5080 a         2976 c         3086 g         5198 t
ORIGIN      
        1 gcgtgttggg attttaaccc tgtttagcgt tttgactgta ccagtcgaaa ttgacgccag
       61 caaccgcgct atcagctggt tacagggcag ttcattggcc ggaaccatgg aaatgggaaa
      121 agcaaaagac gccttaaact gggccggtta cacttacgtt gtagctgctt tgggttcgtt
      181 ggcaactttg atttattaca tcatgttata catgagtaga agataaaaaa gtgcaaaatt
      241 ttcactgaaa atcagcgagt tatgaaggta gctcgctttt ttttgtgcat tttccttgta
      301 ttttgaaagg ggtacgggta tctttgctgc cccgtagata aaacgggctt tttaaacagt
      361 ttaacaaaaa ccatacagtg gatactttaa gttacaaaac cagatcggcc aatgcggcaa
      421 cagttacgca tgattggtgg ctgatagacg ctgaaggcca gacattaggt cgcctgagcg
      481 caaaagtagc tgccatctta agaggtaaaa acaaaccgta ttttacgcct cataccgatt
      541 gcggtgatta catcatcatt atcaatgccg aaaaggtaaa acttaccggt aaaaaaatgc
      601 tggagaaaga aattgactgg tatacaggat accctggcgg acgtaaagtt gccaatccta
      661 aaaccgttct cgcaaaacag ccgcataaat tattggaacg ttctatcaaa ggaatgttac
      721 cacacaataa actcggttct gcgatgtata aaaaattgtt tgtgtatgcc ggtactgaac
      781 atccgcatgc cgctcaaaaa cctaaaactt taaaataaat aaggcatgga aacaataaat
      841 gcagttggca gaagaaaaaa tgcggttgcc cgtgtttttc tgaaaaaagg caaaggaact
      901 attaccgtta atggtaaaga ctataaagaa tatttcgcag ctaaacactt attgcacaat
      961 gttactgatc cactggaaac agtaaacatg gctaacgatt tcgacgttgt tgttaatgca
     1021 accggcggtg gtatcaaagg tcaggcagaa gcagtgaaaa tgggaatttc aagatgcctc
     1081 gttaaaatga acgaagaatt gaaaccggcg cttaaagcaa aaggttttct tacacgcgac
     1141 agccgtatgg tggagagaaa gaaatttggt cttcacggtg cacgtcgtgc tacacaattc
     1201 tctaaacgtt aatcgaaaaa gcgaaatcaa atggaaaaat tatcatataa cgagttattg
     1261 gaagccggtg tacactttgg acacttacgt aaaaaatgga atccgaaaat gcttccttat
     1321 attttcatgg agaaaaaagg tattcacatt atcgaccttt acaaaactct tgaaaaatta
     1381 gatgaagctg ctgcagcgtt aagaaacatt gcacgcagtg gtaaaaaaat catgttcgtt
     1441 gccactaaaa aacaggcaaa agaaacaatg gaagaacaag cacgccgcgt tggcatgccg
     1501 ttcgctaccg aacgttggtt aggtggtatg cttaccaact ttactaccat tcgtaaaagc
     1561 attaaaaaat tgcaaactat cgaacgtatg ttgtctgatg gttctgccga aaacatgacc
     1621 aaaaaagaac gtctcgtgtt aagccgcgaa cgcgataaat tggaaaaagt aatcggtggt
     1681 gtggtacaat taaaccgctt acctgctgct ttattagtgg tagatatcgg acacgaacat
     1741 atcgctattg ctgaagcaca ccgtctcaat attaccactt tcggtatggt tgatactaac
     1801 tgcgatccta ataaagtgga tttcgctgtg ccggctaacg acgatgctgc taaatcaatc
     1861 gctatcgtga ttaaatattt aaccgatgca attgttgaag gtttagaaga acgtgctaaa
     1921 aacaaagaag aaatggaaga tgcacgtcac gatggtaaag atgaattcgg cgataaagac
     1981 gaggaaatca gactggctgg tgaagatgat gctgatggtc aacgcaaagg tccaagaggc
     2041 aaaggcggtc ctagaacggc agacagaaaa cctggtgcac gtcgtccgat gactcagcgt
     2101 aagaaataag tttatcgatt atcatatttc aaaatcataa acccttccac ctgaggcgga
     2161 agggtttttt aaaataaaca aaaaggagaa taattatgtc agttacaatt actgcaggtg
     2221 atgtaaacaa attgcgtcaa atcacaggcg caggtatgat ggattgcaaa aaagcacttg
     2281 gtgaaacagg tggcgacttt gaagctgcaa tcgactatct gagaaaaaaa ggtcaaaaag
     2341 taagtgaaaa acgcgccgac cgcgaaacta acgaaggtgt ggtgatagct gtaaccaatc
     2401 aggatggtac ttttggtact attatgggtc ttggctgcga aaccgatttc gttgctaaaa
     2461 atcaagcgta tatcgatttt gctaaatcaa tttctgatgt ggctatggca aataaagcgg
     2521 aatcaaaaga tgctttatta gctatgactt tagatggtat tactatcgca gaaaaaattg
     2581 ctgaacaagt tggtaaaatc ggcgaaaaaa tcgatatcac tgattatgct ttattatcag
     2641 cagaaaccgt tgttgcttac aaccactcaa atggtaaagt aggtgtattg gtggctatga
     2701 ataaaaaagg tgaagcagtt gaaactgttg gtaaagatgt taccatgcaa attgctgcaa
     2761 tgtcacctat cgctatcgat aaaaatgatg tgccggaaga agttaaaaca cgtgaaatgg
     2821 aaatcggtcg cgaacaagct cgtgctgaag gtaaacctga agctatcctt gaaaaaattg
     2881 ctaccggtaa attagaagca tattttaaag atgctacttt attaaaccaa aaatttgtga
     2941 aagataacag caaatcaatt cgtgatgtgc ttacagcagt agataaagat cttactgtta
     3001 ctgcatttaa acgtattggt ttaggcgctt aatcctacac gtaatatcga tatcttgccg
     3061 tctgaatgta ttttcagacg gcttttttta tgtcagctgc ctgcaacttt ttaacttttt
     3121 tgacaatcat tcttagtaat aatattacct ttatttagaa atttaccggc aacagcaatg
     3181 ttaattcgta attgtttaat tgtactgttt tttatttgct cctcagctgt tgtgattgca
     3241 caacaaactg actatgttgc cgatgaacct gctgagcaat tattatttgt cgccaataaa
     3301 ggccaaataa taaatgaatt tggtttacct gcaaatgatg tactcttttc acttaccaat
     3361 aacggagcca ccatttttgt ttatcgcgat gggttaagtt atcaattcac caagtatcat
     3421 accggtgaat tgcaaaatgt aaatgctttt ctcttcgacc tcgaaaaaga ttttcctttg
     3481 caggaacaaa ttgtcagcac acatcggatt gatgtgaaat ttgtaaatgc gaattcgtct
     3541 attgaagtaa ttcccgaatc aaaatcatta cttgtagaaa attatttcgg cgaacaatat
     3601 ggcaatggaa taaccaatat tcccacattc gaaaaaataa cctgcaaaaa tatttacccc
     3661 aatatcgatt gggtgctata tagtaataac ggaaaactca aatacgattt agtcctccat
     3721 cccggagcaa ataaaaatga tatcactttt caaattactg gaacagatga agcgcattta
     3781 gttgatgcaa cccacatgca aatttcatct cccttagggg aaattaacga caagggtttg
     3841 ttttgttttg aaaaggaatc agcggaaaaa ataaatgctg cttttatttt aaccaataat
     3901 tatttgaagt ttgacattga taattataat ccattacata ctttaattat tgacccggag
     3961 ctcgactggt caacgtttta cggtggtttt ggtgaagatg ccgttaacgg aattgatgta
     4021 gatgctgatg gtaatatata tattaccgga tataccagca gcagcaatgc aatggcgcat
     4081 ttgggttacc ataatagtta tcagggtggt ttttttgatg cgtatgttgc gaaattaaat
     4141 ccggctggaa atagaatttg ggggacttat tttggcggca ataaaggtga ttatgggacg
     4201 gaattagtta tcgataaaaa caacaaaatt tatgtaaccg gttttacctt cagcaataat
     4261 ttcgaaacaa ctcccggtgt gcatcaggaa gcattgggaa ataattacga cgcgttttta
     4321 tttaaattaa atgacaatgg tactttaatt tggtcgacct tgtttggtgg aggaagttat
     4381 gattatgcca gaggtattnn nnnnnnnnaa aaggtgccgg aaacgacgaa tttctcgcaa
     4441 aattttcacc atcaggtttg cgcatctggg caacatatat gggtggcgaa gctgacgatt
     4501 attcacgagc agttggcgtc gatttagata ataatgtgta tttagttgga tatacagaaa
     4561 gtacaacagg aattgccttt tccggttttg ataatacctg gtcaggtaat tatgattgca
     4621 cactcacaaa atataattcc gaaggtgaat taatatggtc tacttatttt ggtggtaatg
     4681 gagatgataa tgcaaacgga attgatttcg acaacaacaa caatatatat atcgccattc
     4741 aaacaggaac aaattcagga ttagcacatt taggttatag gaactcaaat gccggcggac
     4801 aagatgccat gttggctaaa tttaatcctg atggcgaacg tttgtggtct acgtattatg
     4861 gtggtactgc agaagatatg ggaaaagccg ttgctgtttc cggcgaatat gtgtatttgt
     4921 gcggtcatac ttattcgcat tccggaattg cagcttatgc ttatcaaaat acaatcggtg
     4981 gaaatggtga tgcattactc gcaaaattcg atgtgtttgg caatttttat tgggctactt
     5041 atgccggagg tttttgggat gaatttggca gaaccattca aatattaaat gatgaaacca
     5101 taatattcgg cggtaaaaca tttagttggg attatcctgt cacttttggt gcacatcaaa
     5161 tattttatgg tggaggaaca gcagatggat ttattcaacg cgtatatgat tgcgcaagtg
     5221 ctgaaattta ttacgccgat gcggatgatg atggttatgg ggatgccaca gtaagtgaat
     5281 tattatgtga gccaacaaca ggtttcgttg caaatagtaa cgactgtaat gatggcaatg
     5341 catttattta tccgggagct atagaaacat gtaattcaat tgatgacgat tgtgatatca
     5401 taattgatga agaagtaatt acagcattca ttacaccaat gggtccaacc gttttttgca
     5461 aaggaaatac tgtgttgctg caagccactt tgcatccgac ttatttttat caatggaaaa
     5521 aagatggagt gaatattgtc ggcgcaaccg gcggaaatta tatcgttact aaaacaggta
     5581 attatacggt tgatatttct acactcggcg gatgtaatgc catttcaaat agcatcgcag
     5641 taacggttaa taataaacct aacccattca tttttgcaat aggttcaacc gatatatgtt
     5701 taaccggaaa tgcgaaatta aaaacagtac cgtttgccgg aaatacttat caatggtata
     5761 aagacggaga tattattacc ggtgcaactt ctaacatgta tatggcaaca gctattggtg
     5821 gttacagagt aaaagaaaca aatgcttttg gttgctttaa aaattctaat gaaattactg
     5881 tcaccaatac ctgcaaattg gaaaacgata tttcggagaa tagtgtatta caggtatttc
     5941 caaatcaggc aagtaattcg gtgactattt taatgagcga aaatttattt aattccgaaa
     6001 acgttcaatt ggtggtatac gacaatctgg gacaaaaagt atggcaacaa atttcaccaa
     6061 taaataactt gaacccgatg gttgaatgta tattaccatc acagctggca aatggaatgt
     6121 atattgttga actctccgca aataatcaac aatatagaac caccttagtt gtaagccgat
     6181 aattttattc tataattaat tcatcacaaa atatccaggt ttggccacct ttgccgggat
     6241 gccattcagg taccgttccg aaattaattg ctttgaattt gatatatctt gctgttgtag
     6301 catttactgt tgccgtaagc ttttgaattt ctttcgaatc gtaattagtt gtcaccactt
     6361 gattattgat agtcgttaat aacgaaaacg ctttgccatc tgtggatgta taaatttcca
     6421 attgtttcgg catccaaatc caacttctta catcctgcaa aaatccggct ccaactttcg
     6481 taaatgtttg gttgctgcca cagtctaaaa caacttccat atcggtgcct tgccagcctt
     6541 gccacatacc ggttctaaaa tctgcaccac catgtaaacc atcaattaat gaagtagaac
     6601 caccagctgc atattgtttg tcgtatgccg taatgtattt aatggcataa tcattattgc
     6661 ggcgtttgaa tttacctgta actacacctg aacatgtttc tgctttggtg tcaggattta
     6721 ttacacaata ttgcgcagta attatcgcat cactactgat atttagtatg gccgttacac
     6781 ctttttctgc aggatactgt tgattactgc cattcaccga aaattttaat ataccggtat
     6841 catattgggt tgaaacttca actgttgttt tataggtgaa actattgcca gggcctgaaa
     6901 ttacaggtgc aattaatccc aatgttggaa cactatctgt taatggttca gaatttattt
     6961 tttgttcggt aaattcgaat actaactcgc caccctgcat taaggttgca tgtggaattt
     7021 gtagtgatgg caataattgt ttgttccacg aaacactttt taaaaacggg gttttatcac
     7081 ttttgttttt ggtaataatg cgaacttttt tattgttttc taaggtgatt actgctgtat
     7141 cgaaaatggc agaaccaaaa tcatattcgg gttttcccgg acatacctga tataaaccca
     7201 aagcactcat cacataccag gcactcattt gcccacaatc ctcgttgccg attaaaccat
     7261 ccggtgcatt gtgataaaat gatgtcataa tttcattcac ataatattgt gttttccatg
     7321 gcgttgtggt gcgattaaat aaatacgcat aattgtgact aggttcattg ccatgtgcat
     7381 attgtccaat caaaccggta atatcacttt gttgtcttcc tgtagtacct gtattggtgg
     7441 taaataattc atttaatcgc tgttcgaata attcaccacc gaaatttccc tgcggataaa
     7501 ataacgaata ttgccaggca ttggcttctg taaaattaaa attcacttcg gttggattgt
     7561 aaggactata ccaaccacca ttaaaccttg ggcgtgcaaa tccattttcc attacattta
     7621 aataattgta tcccctgttg gtataataat ctatttcact ttttggtgca ttaaccagtt
     7681 tccccatttg cgcaatacac cagtcattat aactatactc caaagttttt gaaacagatt
     7741 caggttcttc attactttca ataaatcctt ttgctttata aaatgataaa ccaaaatggt
     7801 caagatttgc actgtgtttc atcgcatcat aagctttttg taaatcgtaa ttcgtaattc
     7861 cttttacggc agcatctgca attacactta ccgagtgata acctatcata cattcagttt
     7921 cgttgccgct taattcccaa acaggtaatc gtccacctgc atcgtattgc gctaaaaatg
     7981 tttgaataaa atcatttgtt ctgtcctggt ctattatcga cattaatgga tgtaatgcac
     8041 gaaaagtatc ccataaagaa aataccgtgt agttgttgaa ttgttttgac acatgtatcg
     8101 tattatctct gccacggtac tggtcatcca catcttcata aatattgggc gccagcatgg
     8161 tatggtataa agccgtataa aaagtcacca atttattatc ctcagcttgc aatgattttt
     8221 tcgcctctga accgctctgt actttaatct tgcttaattc aaagttccat aaataatcgg
     8281 cttcattttt tactttagca aaatcccaat gtggcatttc agctgccatg tttttccatg
     8341 ctcctgcaca actcaccggt gaaagcgcac acttagtggt aattacatta ttattatttg
     8401 tattaaatgt aacataacct tttaatgaag taccactaat aataatcggt ttgaaattat
     8461 tggcagctgt tttatatgtt ttattatcat caattagtcc cgcaatagaa aatggttgtg
     8521 aaaattctat tgcaaaatac aaccactgat cttttgccca tgatgatgac cttcgtaaac
     8581 cctgaatttt tgttggtgaa attatctcaa tataacttcc ttccaacaat tggtcacgat
     8641 gatttaaatc taaaataata ttggcctgat tggttgcagg aaaagtatat tgatgcatac
     8701 caactcttgt tgaagcagtt aattccactt gaattttttc atcttccaac attacgctgt
     8761 aatatcccgg tgctgctttt tctgttttat gtgaaaatgc tgatgcatac ccctgtttgg
     8821 ggtcattgtt aataatatta gtagtaatga catttccagc tgttggcata agtaaaatat
     8881 cacaataatc cgccacacct gttccactta aatgtgtatg tgaaaatcca tatataatgc
     8941 tgtcagaata atgataacct gaacaaccat cccaatcgtt ataaccatcg ggacgtgtgt
     9001 cggggctcaa ttgcacaaat ccgaaaggta aagttgctcc gggataagta tgcccgtgac
     9061 cgcctgttcc tatgaacgga tttacatatt gtgttaaatt ttgagcctga catagctgat
     9121 agataaaaag gagcagcagt gtatattttt tcatgccatg aaggtaaaaa atgtgactga
     9181 acattctgag taacagtttg ttatttgctc aatgtgtgga ttttgtctac agttttttta
     9241 gtattattgt gaattgaaat tgtagaccct cctcaaaact gttgaattta ttgaggttta
     9301 cattttcact ggtcgtagca ctgtttttgt aacttattga ttgttaacac acaacttaag
     9361 tttaatgtta tttacatatg aaaaaaagtt tcactttatg tatcctcgtg ttaacaacat
     9421 tttatttttc atacggacaa aaagattgtt cgtttgacca caccggaaaa attccaatta
     9481 acgatttggc cggtggttat tttaatggcg tagccggagg tttgtacccc gatggcacca
     9541 atacacgtcc gcccggacat ttagctaaat gtattgaaca cgtttcacaa attcaaccat
     9601 tagatgttga tggttcgcct tctccttcag gtaaaattgt aatgatggga attggggctt
     9661 ctaatcccat gacggaattt aataagttta aggagatttc agaatatttt gagcctttaa
     9721 acgataactt ggaaattgtt aatgcttgtg ttggtggtgt agctattcaa aaaatgtatg
     9781 aagctaccga tacctattgg ttaggtgtag aaaatttatt ggaagatgct ggactttctg
     9841 cattacaggt tcaggttatt tgggtagagc aggaaaatac taacagttat gacaccgcat
     9901 ttaattcagc taccattggt ttggttaccg attttttaac cttgttacgt gtattcaaag
     9961 ataaatttcc aaatgcacaa atttgttatg taagtgccag agcttatgcc ggttttgcag
    10021 accctattga tccggaaatt tctcacggat tattatatcc acgcgattat tttaatggct
    10081 gggcattaaa acgtttagtt gaaaacgcca taaatgctga agaagattat gactttgacg
    10141 gtcctgatgc agaaatacca ctaataacct ggggaactta ccattggaca gatggttcaa
    10201 caccaagaat ggacggatta tttttagact gcgaagaaga tttatctccg gatggtttac
    10261 atttagcagg tcccggcgaa caaaaaatcg gtacttatat gtttgattat tttagtaccg
    10321 acactacggc taaatattgg ttttatgatg aagctaattc aggtataatt aataatccgg
    10381 tgtctaaaaa taccattcaa atttatccta atccggtaaa tggaaatacg ataaattttt
    10441 atgcgggcac tttcgataat tatgaaaata tccaatatca gatttgtgat gccgcaggaa
    10501 aagttatcag cactggaatc ggatacggaa aagataatat tacaattcaa ttacaacata
    10561 ttgcacccgg tgcctattac attactgcaa taggacagca aaatattgca acaggtagtt
    10621 ttattgtaac tcaataaaat taatgcaact ctttccagat aagtgaagcg ctacccaaaa
    10681 tggctgcttc actttctttt aatcccgaag gcacaatcgg gattttattt ttgtatatcg
    10741 gcaacaaata actttcaaaa cttttaatgg tcggtttaaa taataaatca ccggcttgtg
    10801 caagtcctcc aaacaaaaat attttttcag gacgtgtata acaaactgaa tttgccaatg
    10861 cgaagccaag aatttctccg gttttagtaa atgcttctaa tgcaatagta tcgccttcaa
    10921 ctgctttttt atacacttcg gcgctgtcaa tcatattgcc aatgctggtg cggaattcat
    10981 ccagattttg gtgacgaact aattcgtaat aggtttttac aatgccgggt gcactcacat
    11041 attgttcaag gcatcctcga cgtccgcaag ggcattcccg acctttgcga taaacaatag
    11101 tgtgccccac ttctccggcc aaactatcgt aaccgtaagc aatattgcca tttacaacaa
    11161 taccactgcc taaaccagtg ccaagggtga taaaaataaa atctttcatg cccttagctg
    11221 caccaaatat catctctcca agcgctgcgg ctttggcatc attggttaaa gcaacaggta
    11281 aggaaaatgt tttttcgaaa tattctttta gcggaattac acctttccag ggcaaatttg
    11341 gcgcaaaatc gatagtgccc ttgtaataat tgccatttgg agtgccaata cccacgccta
    11401 tcgtttttag ttgaagcgcc gaacattgtt cattgctcca ttttaacagg tcagcaggca
    11461 gcatatcagg atttgggtaa ccctttgttt tcgccttatc gctggctaca atattgccat
    11521 cacggtctac taatccgaat tttattccgg ttccaccaat gtctatacct aaaactactt
    11581 ccttcatcca gctgttttgc ggtaaaatta aattatttgt tcaatagtct gcgatgataa
    11641 ttgatttgtc ccaggtgata gtctaaatgt gccaacaaat aaacaaacac ataaccgatg
    11701 gtttttcctt ccccaaattt gtcgatagga taaattttat tgaggtcgtc atcctgcaaa
    11761 cccgaaaggg tagtgctcaa catggtatta gtatcttgta gtaaattgac caattcttgt
    11821 tgtggtattc ctttagcact gaactcggca tctctgtcgc gcacataacc ggttttaccc
    11881 aaaatgttgc caataaaata atttaaatta cccactatat gcaaagccag atttcctgca
    11941 ctgttgttga tgccttcttc agttttccaa atatcatctt cgttggtaaa tgctgatata
    12001 tcagtaatcg tcatttgcat atgccggtta tatacttcaa gtattgcctg aatcatatag
    12061 tttaattttt gaataaaatt aaggaattat taatttgttg ctttgagccc ataataataa
    12121 ctccgtctcc cacttttatt gacgcatcag gaccggggtt taaaatatat ttattttcac
    12181 cggatttaat accaataaca gtggcaccag tttttttcca ggtatctatt tctcctacaa
    12241 caacattttt ttgagaggtt atttcctgaa ttaaaaatga gtcaccggta gaagtggaca
    12301 tgatatctat aaactcttca atatcaggat tattcaccag catagccatg tgtgcaccac
    12361 caattttatc gggcatgatt acattttttg ctccggcatt ttttaatttt ttaattgaac
    12421 tcacatgtgt tgccctgctg ataattttta tgtttggatt gagttctttt gctgtcaaac
    12481 aaacaaaaac attgagcgca tcatcaggta aagtactaat tatggcgcta gcctgcatga
    12541 tgccggcact caataaggtt tcatcacgtg tagcatcggc ttctagaaaa taataaacag
    12601 gatctgcatt ttcagtgtgt agtttacttt gctcagtttc tataatcacc acctgattat
    12661 tagtttgttt taaaacacgt gctgcttctc tgccatttct gccaaaacca catattatag
    12721 tatggtcttt taactggctg atttcttttt gcattttata aattttaaaa tagcgaataa
    12781 attctccgtc caaaaaatat cttgaaattt gggtgataaa aaatccaaat atgacaagtc
    12841 ctaacacaat taaaaaaatg gtaaatattt taccttcagg cgaaagtgga ttcacctccg
    12901 aaaaaccaac agttcctaat gtaatgatgg tcatatacaa agcgtccaga aagttgtagc
    12961 cttcaattag gataaatcct attattccaa tggaaattgc tccaattaat aaataaatgg
    13021 ccaaatacgt tttacggaaa ggattattgc cgcgttttgg aaaattgctc atgaactgtt
    13081 aaacaattat tgaatgccta aatatactac tataaacccc tatgtaacta catacaaaaa
    13141 tcaccgtttg tgattaatag attgccgaga taaatacagg taaacatatt tggagggagg
    13201 gggctattat gaaaaaagcc ttaggttcgg ttataaggta atatgcatta tgagggcttc
    13261 atttttgaca ttatttcatc aaaatctgca atttttcacc gtttttttta gcgctgtttc
    13321 attgggataa agaaatttat gaatccgcag catatttatc ctttgttttc gtcaaatact
    13381 ggtattagtg gtgtaattgg ctttcgtttt tgcaaaacca gatattatat tagatttttg
    13441 cacctcaatt gaacgatatg gacttttcaa tatttttaac cgctgatgct tggattgctt
    13501 tgctaaccct tactgtttta gaaatcgtat tgggtatcga caacattatt ttcatcagta
    13561 ttttatcaac caaattgccg gcggagcaac aaaaaaaggc ccgtagatgg ggtttagggc
    13621 tggcaatggg aacgcgaatt ttgcttttgc tgtccattag ctggattctg ggactggagg
    13681 cagacttatt cagtattgcc ggtcatggta tttccggacg cgatttagtg ctgctttcag
    13741 gtggtttgtt cttgatttat aaggcaacag tcgaaatcca cgctaaaatt gaaggagatg
    13801 agcacgaaga tagccctaac atcaaaaaac cttctttcac ttcggtgatt ctacaaattt
    13861 taatgctgga tatcgttttt tcactcgatt ctgttattac tgctgtaggt atggttgacg
    13921 ccgacaaagt aatcatcatg attttagccg taattattgc ggttgctatt atgttgttcg
    13981 cggccgaccc tatcagtaat ttcgttcaaa aacatcctac agttaaaatg ctggcacttt
    14041 cgtttctagt aatgattggt gttagcttgt tgattgaagg tagtggcggt cacgtggaaa
    14101 aaggatttat ttacgtggca atgggcttct cggttgtagt ggaaatgctc aatttgagaa
    14161 tgttgtcaaa tgccaaaaaa cgcaaggaaa aaaagaatct agaagagaat gaattcggga
    14221 attaatttaa cccgttaaat aataatttca atcccggtat gtatatgccg ggattttttt
    14281 tattcatttc tgaataacca tttttgtctc gcagccatat atgccgggaa ataagaggca
    14341 attccaccta taattaatac agttagaaat acattcacat aatctttcca ttctaacgct
    14401 ataggatatg cagtaacaac aaatgtacct tgcccgggaa tggttactaa accaaattgt
    14461 atttgtaaat aacataatat cgttgccagc gaaaaaccta ctgctatact gactaacgtt
    14521 tgtaatacac cctgaataaa aaaaatattg cgcaccgatg cttcagttgc accaagtgaa
    14581 cgtaaaatac caatatcttt tttcttatcg attaccaaca tcgctattga gccaatcata
    14641 ttaaatgccg caacaataaa aataaatgtc aaaataaaat acaccgccca tttttctgtg
    14701 cgcattaccc gatataaaaa ttcattttgc atgaatctgt tttttaccaa ataatcatcg
    14761 cccatgatgg ctgtaatctc ctccataata tcatcaacct tatttttaga ggatgttttt
    14821 atttctaaag aacttatttc tccattttga taatctaata atccttgaat aaactgcaag
    14881 gatgtaatta cgtatttgct atcaaattcc tgttgaattc taaatacacc cattggctga
    14941 atgcctcgct gtacaaaggt gttgcgagga ttgctggagg atttcacccc tctgcgcgga
    15001 ataaatattt gcacagcagg ataatctaat cccaaactta catttaatgt agaagcaaca
    15061 cctgacccca tcacggcaca atcgagattg taatttaaat aggcataatc taaaatgtaa
    15121 tcgccataat aaacactgtc gtcaacagca gttacataac gatacatggt gtctacacct
    15181 ttaatggttg cgatgtgttc ttgtttattg tatgccaata aaacattttc ttcaagggtt
    15241 ttggaaatgt attcaacacc ttcaattgat tgaatttttg ccagtttatt gctgtctatt
    15301 gcaaacactt ttcctgttgc aggagttatg gtaatgtcag gataaaaggt gctgtataac
    15361 ctggcaacta agttttcaaa accgttaaat gcagataaaa caataattaa ggacgcactt
    15421 ccaataataa ttcccgacat cgaaataccg gaaataatat tgatggcatt agtcgatttt
    15481 ttagaaaaaa tataacgcca ggctatcctt aaggtaagct tcatgcaggc ggattattgt
    15541 tgtcttcctt cgttttttta aataattctt ccaaccgata aacggtatct agtgtgtcgt
    15601 cgagaaaaaa ctgcatttca ggaataatac gcaacgaatt acgcattatg tgcccaaatc
    15661 gcctgcggat atctctgata ttactattta aagaatctac taccttttcc ccttcgtttt
    15721 tatcgaaaac acttacgtga attttgcaat tttgaagatc ggaggtaata ctcacatctg
    15781 ttacggtcac aaacttatta ccgtaataat tggccccttc tttttgcaaa aaagtgctca
    15841 tctcatcttg tatcagctgc ccaatcttct tttgtcgtct gctgtccata tggtgctaag
    15901 ttaaggtgtt tcagccaatc gtttatagcc agaggtagat tttgtaaatt tgtggggctt
    15961 atgaaaagtt atctgaggat attgaaatac gtgggtagat tcagaatcta tgcaattctg
    16021 aattttgtgt tcaatctgtt ttatgcattt tttaacctgt tttccatcct gatggtgatc
    16081 cctttcttgc aaatcatctt taagatggtt acaccaccaa catctaggcc tgaatttagg
    16141 tggaacataa gggaaatgct gggaattttg aactatgagt ttgccagtta cgtcaatgct
    16201 catggcgcgt attcaggact tatccttgtc agtatcgggg ttgtttttat gttcctgttc
    16261 aaaaacattt ttcgttacct ggccttgtat tttttagcac cacttagaac cggtgtagta
    16321 cgcgaattga gacaaggggt atttgacaaa
//
LOCUS       DAORKW010000024        15751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_42_length_15751_cov_5.027013, whole genome shotgun sequence.
ACCESSION   DAORKW010000024 DAORKW010000000
VERSION     DAORKW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15751
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42_length_15751_cov_5.027013"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..769)
                     /locus_tag="PLB46_02125"
     CDS             complement(50..769)
                     /locus_tag="PLB46_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRF75338.1"
                     /translation="MGDNNKHNAKLGLFITIGLVIFIVGILAIGNINNAFKKSIQVFA
                     VFEEVNGLQVGDNVWLSGVKVGTVKDMQFLSNSDVLVNINIEQKIQQYIPKNALAKIS
                     SDGLIGNKLIVIYSGDLQSGMLQSDDTLHVESSITNEDMMATLQQNNTNLLAITSDFK
                     NISKQILEGKGTVGKLLTDDQLYTKLSSAMTEINTTAAKLNSVSGSLEQFTQQLNKPG
                     NLANTIVTDTAIFKDVELMVL"
     gene            complement(771..1526)
                     /locus_tag="PLB46_02130"
     CDS             complement(771..1526)
                     /locus_tag="PLB46_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF75339.1"
                     /translation="MTSVENATINIKGLSKSFGNLHVLSNIDLILNKGEHIAILGKSG
                     SGKSVLIKIIAGLLLPDTGTVLTLGQNIATITEKEKRNLRLKIGFSFQNSALYDSMTV
                     RENIEFPLVRKDKHLTQLEINKQVDAVLDAVGVRVKAEKMPSELSGGQRKRIGIARTL
                     ILEPELMLYDEPTAGLDPITCIEINNLIIELQKRYNSSAIIITHDLTCAKTTANKVVV
                     LLEGQFVRQGTFKEVFETDIQIVKQFYDYNFII"
     gene            complement(1532..2329)
                     /locus_tag="PLB46_02135"
     CDS             complement(1532..2329)
                     /locus_tag="PLB46_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF75340.1"
                     /translation="MSDKTKKFFISAKADAFFIGITSAVQFVKRYFYEAFRGPWEFAE
                     LKKQCYLMGIKSLPLITLTGFVTGVVFTKQSRPSLQELGAVSWLPSLIAIALIRALAP
                     LITSLISAGKIGSNIGAQLGSMRVTEQIDAINVSGVNAFKYLVVTRVTASTIMIPLLM
                     MYFAFIGLFGSFINVYMGENTSFISYFQEAFSPISFLDVFTGLFKSILYGFTIGIVGS
                     YKGYNASKGTEGVGKAANQAVVISMFLIFIEEIIVVQLSAWIRLSLN"
     gene            complement(2464..4905)
                     /locus_tag="PLB46_02140"
     CDS             complement(2464..4905)
                     /locus_tag="PLB46_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRF75341.1"
                     /translation="MRIPKRNRKNLKIAILTISIALAIYITTAVLLLTIFKDKIQEQV
                     TATINDNINGEVKIGSFSINILSNFPNASFTIKDFQIANQATNLNKNEIINVKYIYLH
                     INLIKLITGKVELKRIIVKNSSIHIYRLTNGLKNTDGLIKRSEDKGENNINSTLNKLN
                     ITVSDVNIIYEDTIVACNFFDFTITHADVQINSSTTGFYVSSKNTIATHQIIFNKDRG
                     SALQDRKLQMQMMLDWNTKTQTYTILPTRVKDKNESFTLLGSISLQETKALKLIIYNH
                     SITLQNAVVLATPYAREKLTGFDFEDHMSAQMVVKSNLIPNLPPDIDIYFNSSNNTFS
                     GKNKKLNNIDFSGHYMNHLNDTLFNDETNNAVFFDTISGYYEGIPFNGKFTLNDFNNP
                     AIDLRVVCNTKLAVINNAIDTSLIRFNAGEMALDLRFKGALAKNDTLPVDTNAIVNGR
                     LSLTKADYVIPKNNYHFKNITGSLLFDKQDLLISNLPVTLNNNKITISGAFKNISNNI
                     IHPEAPLYADISVLAQEFNINNFPPPKEKPAKRSKQNTITQNTELLLNSLKADISLKA
                     NQIIYKNLLMSNASGIFTINNNSIHCREASGYISDGKAILSGNLNNLGNSTMPFDLQV
                     QLSNIDIEKIFKGLNNFNQNAVTSEVIDGKVSATISLSGNLNKQFQPVIPTLNGNVDI
                     KVKNGALHNLQALQEITTVIFKNKDLQDIQFATINHKSKVKNGDVLVEEMNINSSLII
                     FMIEGTYSFDNNTELYFKIPLKSLKNADEKYLEPNKVKDEKTGISLLFKLTKTDGKAK
                     ITPVLFKREKNQKQE"
     gene            complement(5048..6484)
                     /locus_tag="PLB46_02145"
     CDS             complement(5048..6484)
                     /locus_tag="PLB46_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF75342.1"
                     /translation="MHKLLPILLPILSLLVMTSTNAQVYEYTNDPIGFPSYHNPNFYA
                     ERLLRSNEAAGAGDACDIGFSTKHFSTEAVFEDNIAGVVLKLIPDSLVTLNISSISAA
                     FRITEKGPIYARYSYSLDSGVTWVNQGSDITPIINDCGGSTINGTWDMDDFSSTELIL
                     IKISGYNALDSVGRLNITTMNIYGTVDIIDEDGDGYGIYIDCDDTNPLINPGATELCN
                     DIDEDCDGISDELSSTITPTGIINICKHDFVELYAPSGYTSYQWLKNGYIVPGATDSI
                     LSTEKPGYYQVVIEDGVCTDTSLVQAVAVKENPFANIYSPDGIDLCLDDTLKIKASYG
                     ATYTWQWYKDGIAIPFENFYKMQVVETGAYYCNIVTFYGCSRVTDTLNIVKTCRLSEE
                     TNSNFQIYPNPVVNNLNVNITIKSTEETDASLQIINLEGQVVNNYFYPMNNGILHVNL
                     PVTHLQPGVYIIKAYINKEIISKIFVVS"
     gene            complement(6550..7677)
                     /locus_tag="PLB46_02150"
     CDS             complement(6550..7677)
                     /locus_tag="PLB46_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laminin B domain-containing protein"
                     /protein_id="HRF75343.1"
                     /translation="MKIFKILILIICLTVALFNVQGQPANYFTVDAEGWTAQAEGLGI
                     DPIASWAALNGNPMGCYKGTDNASGTFYFNSSIAYGTDLSPFYGAQLFFDLKQNANTF
                     QTNEPDVMICKADGTKIVYNTSANPGTSWTTYAVPLLETGWKYNSLAGLPVTYSDMLT
                     FLEFVAVIKIRGDYSSITTETNWLDNVKIINPILLPITLISFSGNQYTNAQCELKWLT
                     SSELNCNYFQIEKSVNVGANYDSIGSQRAVGNSNEITSYKFIDHLFSNSAYYRLKTVD
                     TDYTNRYSDQIYVEHLLAPLAISVYPNPSNGMIHLMNENGFELAAKISVFDIQQNLVY
                     TTSVQTICNQITLPLTNLTSGIYFISVKSDNQITTYSAQIL"
     gene            8401..9729
                     /locus_tag="PLB46_02155"
     CDS             8401..9729
                     /locus_tag="PLB46_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF75344.1"
                     /translation="MFRTIQNCLFACALIFSLLVACKKEKVEEITAFTPVPTSDIVMY
                     EVNIPAMSTTRDINGVIARLDIIKELGINTIWLMPIFELGELNAFGSPYCIKDYKAVN
                     PELGTFADLQLLVEEAHARGIAVILDWVANHTSWDNAWITNDGWYSEDASGNIISPLG
                     TTWYDVADLNFTNADMRLAMIDAMQYWIDNANIDGFRCDAADYVPFDFWQQAITSLNE
                     NTERDLILLAEGARADHFTAGFELNFSWSMLGTLKNVFTGGGYVSTIFSTNTAEYISV
                     PDGSKKLRFTTNHDETNIAPPATAYGSQDAALAAAVIAFYLQGVPLVYCGQEVGISSA
                     SAYNGALNWTINPDIYQTYKDLLKNYNTYSALRYALPETFSTATVAIFKKQLNDENMV
                     VIVNTRNSNQNAPVDATLQGEYEDVMNANTLILDDNITLSPYQFYILKRK"
     gene            complement(9764..11323)
                     /locus_tag="PLB46_02160"
     CDS             complement(9764..11323)
                     /locus_tag="PLB46_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75345.1"
                     /translation="MKNRIYLMFSGILCAQFLIAQEIAPVIDWQKVYGGSNVDFIRDF
                     IAIPDGYVGVGYSGSTDGDLPPEIGGYYDAWMIKLDANGNVIWSKTYGGSENDVFESV
                     VQANDGGYIVLGVTASADGDLSTPIGNNDFWVTKVDSNGNLVWSSVFGGTAAENAYDI
                     IALQDGNYLAVGSTNSIDVDAITSHGPAEVSDILVVKLNGSGGKIWSKCYGGTLSESG
                     HEALETNYAKIIIVGYASSSNGDITEVKGAMDAWVIKIDGDDGALYWETTIGRWNNDI
                     AESICKTPGGDFLVAGRSNSSFPGNHGEIDVFVSRLTALGEIKYTKVYGGNNNDVADK
                     IVADNEGNYIVTGYTESITSGDVSGKHKGTDVWMFNMDLAGHLMWQHCYGGNRNEKAY
                     ESLVLADGSIVFLAQTNSFNGDVGYNLGDYDTWIVKTEPTTMRMQQSAVEEINISPNP
                     SSYMLNVCANYADIKTITIYTAQGKQVWYSQTSEAQLKIPVFNLPAGMYYVQVSACNH
                     KTIIKQIIIEH"
     gene            11577..>15751
                     /locus_tag="PLB46_02165"
     CDS             11577..>15751
                     /locus_tag="PLB46_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HRF75346.1"
                     /translation="MATLLVKGRAFTELTRKIDPELNVAGAYPEKQILLANDREIGAL
                     QHELDIEADDVFEVETTDNVTWVLTGEELLGKFASTANRTRAANNKSGDVFELNASII
                     FPTEERGIGNVINLRSIIKWAFKRAIKEIKIINGLVEIVDKKLVENPGLFRAERLLKN
                     ALKEQIKSATQLSDTEPNLIFIHGTAANVEMTYGKLTPEGDADWLEVQKRFGKRIYAY
                     QHHTISKSPLDNAVELIALFPDTFKLHLVTSSRGGLVGELIFATAYYKDFPVMLDTLK
                     MQLAAAKDRADDVKNVEQLIQFGKTKKIEILDYNRIACPANGTILASGRLDKFFLIVL
                     NAIKLIPGIGGNPIYEAISTALLNLINAKADCSQMPGLEAMMPESPFIKALNSADVEV
                     DHTLKIIAGDTERSKIFRAMAVLLSDIYYRTEHDFIVNTNSMFCGYKRKNTQYIYHKS
                     GAVSHFNYYYNNQTRNPLYAALKGVENIIEFSVLPNGLNFRSPSFSFTAYLENTRGYY
                     KNKIVVTRDEQELEFESEAVIQKLDVKLTHGDLGFAEYPLIVGHFEGDGIVSSEKAVD
                     KHMDRRLLEMHLAGIYPGEVETSAVLLKEGKGFQGAVVVGLGSSGELSGESLAASVAH
                     GAVNLAFRYANADKKIHQIGISTLLIGSGYAGISIKTSIRSIIDGISRANRRIKKINN
                     GAVPFITKIQFIELYEDRVMMAAKFINNTIINETPENFVQTKVEIDQAPGFQKRLFDN
                     STSEWWYRLQILTINNEKSVNKQLRYVLLNNGAKVEEMLVYTQRILIDKIIDAAIHQQ
                     NIELGLSKLLFNLLVPNSIKDFTQEKRNLLLIVDAETAAYPWELMYNPVKEGNKPIAV
                     NSGMIRQLIVESPHKPVLHVAGNTALVIGDPKVSEGWTQLEGARAEAQAVQQVLEQGG
                     YKVNALIDKDFMAVISALHNNPYKIMHFAGHGDVDLEDPKNSGMILDNSIRITPSEVA
                     QLDYIPELVFINCCFLGSTHQVKVQWNQLAANLGTQFINIGVKCVVAAGWAVNDAAAE
                     EFAKTFYDMMLTGCEFGEAVQAARQKIFEDDRFSNNTWGAYQCYGDPFYKLDPLSHGK
                     SGKSNNYVNIQECIVALNNLIQRSDPRSTRSKSTAVVSAINAHVESLDKSWFKDVQLL
                     EAIADSYYAIGEWATALTYYEKLYQIATANFKLDSLYRFSKIAMKIGVEKAKTGKTKE
                     EKVTGENWIKLAYKIAENINNIAENAYTLLLIAGITKQHAYLFKGVQRINKLKAAAAL
                     YEKAFRVRYEAGQGADYYPLHNWLTIYELLKWYRIKLKVNPQEITQFIQDANNTKKSK
                     KIKLDLFRYHSAEAEFEIYQLLAATKLADKKEKIAEGIVKHYKIGWDYGGTYYKTVAI
                     LEQINFIVEMMKEA"
BASE COUNT         5059 a         2607 c         2814 g         5271 t
ORIGIN      
        1 aaagttaaga tttttgtcta cttttaaaac gtactatttt tgggggagct tacaatacca
       61 tcaattcaac atctttaaat atggcggtgt ctgtgacgat agtatttgca aggttaccgg
      121 gtttgtttag ttgttgggta aattgctcca atgaaccact cacagaattt aatttagctg
      181 ctgtggtatt tatctcagtc atggcacttg aaagtttggt ataaagctga tcatcggtta
      241 atagttttcc aactgttcct tttccttcta aaatttgttt acttatattt ttaaaatctg
      301 aggttatagc caagaggttg gtattgtttt gttgtaatgt agccatcatg tcctcattag
      361 tgatactgct ttccacatgt aaagtgtcgt ccgattggag cattcctgat tgcaaatcac
      421 cagaatagat aacaattaat ttgttgccaa ttagtccatc agaactaatt ttagctaatg
      481 cgtttttggg tatgtattgc tgaatttttt gctcaatatt gatatttacc aatacgtctg
      541 aattgcttaa gaattgcata tcttttacgg tacccacctt cactccggaa agccatacat
      601 tatctcccac ctgtaatccg tttacttctt cgaatacagc aaatacctga attgattttt
      661 taaacgcgtt attgatgttt cctattgcca gaataccaac tataaaaatc actagtccaa
      721 tggtaataaa aaggcccagt tttgcattgt gtttgttatt atctcccata ttatataata
      781 aaattgtaat cgtaaaattg ttttactatt tgaatgtcag tttcaaatac ttctttaaat
      841 gtaccttgcc ttacaaattg cccttccaat aaaacaacaa ccttgtttgc cgtagttttt
      901 gcacaggtaa ggtcgtgagt aataattatt gcacttgaat tgtaacgttt ttgcaattca
      961 attataagat tgtttatttc tatacacgta atagggtcta accctgcagt aggttcatca
     1021 tataacatta attcaggttc aagaattaat gttcgagcga taccaattcg ttttctttgc
     1081 cctccggaaa gttcagaagg cattttctct gctttaactc ttacgcccac agcatccaac
     1141 actgcatcaa cctgtttgtt tatttccaac tgtgtgagat gtttatcttt gcggactagc
     1201 ggaaattcaa tattttctct tacagtcata ctgtcgtaca atgcactgtt ttgaaatgaa
     1261 aatccaattt ttaaacgcaa gttgcgtttt tctttttcag taatagtagc aatattttga
     1321 cctaaggtta atactgttcc tgtatcaggc aataataatc cggctataat tttaattaaa
     1381 actgatttac cactcccaga ttttcctaaa attgctatgt gttcaccttt attaagtatt
     1441 aaatcaatat ttgacaatac atgtaaattg ccaaatgatt ttgataatcc cttgatgttg
     1501 atagttgcat tttctacaga agtcatagca attaatttaa agataatcgt atccaggccg
     1561 aaagctgcac aacgattatt tcttcgataa atatgagaaa cattgaaatt actacagcct
     1621 gattcgctgc tttaccaaca ccctcagtgc ctttggaagc attataccct ttataacttc
     1681 caacaatacc aatggtaaat ccatataata ttgatttaaa taaaccggta aatacatcta
     1741 aaaaagatat tggtgaaaat gcttcctgaa aataggatat aaaactggtg ttttcaccca
     1801 tatatacatt gataaaagaa ccaaacaatc ctatgaacgc aaagtacatc atcaataatg
     1861 gaatcattat ggtagatgct gttacacgag ttactactaa atatttaaat gcgtttactc
     1921 ccgatacatt tatggcgtct atttgctctg ttacgcgcat tgaacccagt tgtgcaccta
     1981 tatttgatcc aattttacct gcggaaatta acgaagtaat aagtggtgct aatgcgcgta
     2041 ttaaggcaat agcaattaat gatggtaacc atgaaacagc acctaattct tgtagggatg
     2101 gtcgcgactg cttcgtaaaa actacgcctg ttacaaatcc ggttaaagta atgagtggta
     2161 acgacttgat gcccattaaa taacattgct tttttagctc ggcaaattcc catggacctc
     2221 taaaagcctc gtaaaaatat cgtttaacaa actgcaccgc tgaagtaata ccaataaaaa
     2281 aagcatcggc tttagctgaa ataaagaatt tttttgtttt atctgacata aagattaaag
     2341 caacatgaat tgaatcgata cagtttgaaa gggtcgtaac gaaaacaaat gatgaaacat
     2401 cacttttata aagtaaccac tttttaaaag gtaaatgtta tactatttca tttaatagtt
     2461 atattattcc tgcttttggt ttttctcgcg tttaaataac actggagtaa ttttggcctt
     2521 gccatctgtt ttggtgagtt taaataatag ggaaataccc gttttttcat cctttacctt
     2581 gtttggttcc aggtattttt cgtcggcatt ttttaaactt tttaatggaa ttttaaagta
     2641 gagttctgta ttgttatcaa agctataggt tccttcaatc atgaagatga ttaaactgct
     2701 atttatgttc atttcttcta ccagaacatc cccatttttt actttagatt tatgattaat
     2761 ggtcgcaaat tgaatatcct gcaaatcttt atttttaaaa attactgtgg taatttcctg
     2821 taatgcttgt aagttatgta aagctccatt cttgacctta atatcaacat ttccatttaa
     2881 tgtaggtata acaggttgga attgtttatt gaggttgcca cttagtgaaa ttgtagccga
     2941 tacctttcca tctataactt cagatgttac ggcgttttgg ttaaaattat ttaaaccctt
     3001 gaatattttt tcgatgtcaa tattgcttaa ctgaacttgt aaatcaaaag gcattgtcga
     3061 attgcctaaa ttatttagat tgccggataa aattgctttc ccatcagaaa tataacctga
     3121 ggcctcacgg caatgtatac tattattgtt aatagtaaaa ataccgctgg cgttgctcat
     3181 taataaattt ttgtaaatta tttgattggc ctttaatgat atgtcggctt tcaaactatt
     3241 taataataat tcggtatttt gagtaatggt gttttgtttt gagcgttttg caggtttttc
     3301 tttggggggt ggaaaattgt tgatgttaaa ttcttgcgct aacactgaaa tatctgcgta
     3361 aagtggtgct tcagggtgaa taatattatt acttatgttt ttaaacgcgc cggaaatagt
     3421 aattttattg ttatttaaag taaccggaag attggatata agtaaatcct gtttatcaaa
     3481 cagtaatgaa cctgtaatgt ttttaaagtg atagttgttt ttagggataa cataatcagc
     3541 tttggttaag gaaagccgtc cattaacaat agcgtttgta tctacaggca aggtatcatt
     3601 ttttgccaat gctcctttaa aacgtaaatc caatgccatt tcacctgcat taaacctgat
     3661 taaagaagta tcaatggcgt tattgataac agctaatttg gtattacata ctaccctaag
     3721 atcaatggca ggattgttga aatcgtttaa tgtaaacttt ccattaaaag gaatgccttc
     3781 ataataaccg gaaattgtat caaaaaacac agcattattt gtttcatcgt tgaataaagt
     3841 atcgttcaaa tgattcatat aatgcccgga aaaatcgata ttatttagtt tcttgttttt
     3901 gcctgagaat gtattgtttg aagaattgaa ataaatatcg atgtccggcg gtaaattggg
     3961 tattaaatta cttttaacaa ccatttgtgc gctcatgtgg tcctcaaagt cgaaacctgt
     4021 aagtttttct cttgcgtagg gggtcgctaa tacaaccgca ttttgcaagg tgatgctatg
     4081 attgtaaata attagtttta gagctttggt ttcctgcaaa ctaatgctac ctaataatgt
     4141 aaatgattcg tttttatctt taactcgtgt tggtaaaatg gtgtaggtct gcgtttttgt
     4201 attccaatcc aacatcattt gcatttgaag ttttctatct tgcaatgcag agcctctgtc
     4261 tttgttaaaa atgatttggt gagtggcaat agtgtttttg ctacttacgt aaaatccggt
     4321 agtagaggag ttaatttgaa catctgcatg ggtaatggta aaatcaaaaa agttacatgc
     4381 aactatcgtg tcttcataaa taatgttgac gtccgataca gttatattta atttgtttaa
     4441 ggtcgaattt atgttgtttt cccccttatc ttctgacctt tttattaaac catctgtatt
     4501 ttttagtccg ttggtcaatc tgtaaatatg tatagatgaa ttttttacaa taatgcgttt
     4561 aagctctact ttgccggtaa tgagtttaat aagattgata tgtaaataaa tatattttac
     4621 attaataatt tcatttttat ttaaattggt tgcctgattg gcgatttgga aatctttgat
     4681 cgtaaacgag gcattgggga aattggaaag aatatttata ctaaacgacc caatttttac
     4741 ttcaccattg atgttatcat ttattgttgc agttacctgc tcctgaattt tgtccttaaa
     4801 tattgtgagc aacaaaactg cagttgtaat atagatggca agtgctatac ttatcgttaa
     4861 aatagcgatt ttcaggttct ttctgttcct tttaggtatc ctcatttatc gtattaaaag
     4921 catccaacaa tttacatttt actgcgcttt gtcggatgct tagtttatct aaatccatgt
     4981 gtgttttaat gggaattaat cagcatttag ttcattattt aaaaccatta atatcactaa
     5041 ataatgttta agatacaaca aatattttac ttattatttc tttattaata taagctttga
     5101 taatatatac acctggttgt aaatgtgtta caggtaaatt aacatgaagg atgccattat
     5161 tcattggata aaagtaattg tttacaactt gtccttccaa atttataatt tgtaatgaag
     5221 catccgtttc ttctgttgat tttatagtga tgttcacatt taaattattg acaacaggat
     5281 ttgggtaaat ttggaaattt gaattagttt cttcacttaa tcgacatgtt ttaacaatgt
     5341 tgagcgtatc tgtaacgcgt gaacatccgt aaaacgtaac gatattacaa taataggcgc
     5401 cggtttcaac cacctgcatt ttgtaaaaat tttcgaatgg tatggctatg ccgtccttat
     5461 accattgcca tgtgtatgtt gcgccataac tagccttaat ttttaaagta tcatcaagac
     5521 ataaatcaat gccatcaggt gaataaatat tcgcgaatgg attttctttt acagcaactg
     5581 cttgaaccaa agaagtatct gtgcaaacac catcttcaat tacaacctga taataacccg
     5641 gtttttctgt tgataatatt gaatcggttg cacctggtac aatataaccg ttttttaacc
     5701 actggtatga ggtgtaacca gatggcgcgt ataattctac aaaatcatgt ttgcatatat
     5761 taataattcc tgttggtgta attgtggaag ataattcatc actaatacca tcgcaatctt
     5821 catctatatc attgcataat tcagttgcac ccggatttat aagcggattg gtatcatcgc
     5881 agtcaatata aataccgtat ccatctccat cttcatcaat tatatcaact gttccataaa
     5941 tattcatcgt tgtgatattt aaccggccaa cagaatctaa tgcgttataa cctgaaattt
     6001 ttattaagat taattcagtc gaactgaaat catccatatc ccaagtacca ttaatagtac
     6061 ttccaccaca atcattaata ataggagtga tatcagaacc ctggtttacc catgtaactc
     6121 cggagtctaa actataagag tatctggcat aaattggtcc tttttctgtt atacggaatg
     6181 ctgcagaaat agaggaaata tttaatgtta ctaatgaatc tgggattaat tttaaaacaa
     6241 cgcctgctat attatcttca aacacagcct cagtagaaaa gtgtttggta ctaaaaccaa
     6301 tgtcacatgc atcgcctgca ccggcggctt catttgaacg taataaccgt tcggcgtaaa
     6361 aattggggtt atgataagat gggaaaccaa ttgggtcatt ggtatactca tatacttgag
     6421 cattggtaga agtcattacc aataacgaca atatcggtaa taaaataggt agtaatttat
     6481 gcattttagt tgatttgaat tggttcctga taattaaaat taataccagg taaaaaagga
     6541 ttgttgatgt taaagtattt gtgcgctgta tgtcgtaatt tgattatcac ttttaaccga
     6601 aataaaatat attccgctag tgagatttgt taatggtaat gtgatttggt tacaaatcgt
     6661 ttgaacacta gttgtataaa ctaagttttg ttgaatatca aaaacggata ttttagcagc
     6721 taattcaaat ccgttttcat tcattaaatg tatcattcca ttcgaggggt tcggataaac
     6781 actaatggct aaaggcgcta gtaagtgttc aacatatatt tgatcggaat acctattagt
     6841 gtaatcggta tcaaccgttt ttaaacgata ataagcgcta ttactaaaca gatgatctat
     6901 aaatttataa cttgtaatct cattcgaatt tcctaccgca cgttgacttc ctatggagtc
     6961 gtaatttgca ccgacattta cacttttttc aatttgaaaa taattacaat ttaattctga
     7021 tgaggttaac cattttaatt cacattgtgc attagtatat tgattgcctg aaaaactaat
     7081 taatgtaata ggtaataaaa tagggttaat tattttaaca ttatccagcc aatttgtttc
     7141 tgtggtaata gaagaataat cgcctcttat tttaattact gctacaaatt ccaaaaaagt
     7201 taacatatcg ctgtaggtga ccggcaaacc agctaacgaa ttgtatttcc atcccgtttc
     7261 caataatgga acagcataag tagtccagga agtcccggga ttagcactag tattatacac
     7321 tatttttgtg ccgtctgctt tacaaatcat aacatcaggt tcattggttt gaaaagtatt
     7381 ggcattttgt tttaggtcga aaaataattg tgcaccataa aatggagata aatcggtgcc
     7441 ataggctata gaactattaa aataaaaggt gccggaagca ttgtctgtcc ctttataaca
     7501 acccattgga ttgccgttta aagctgccca tgaagcaatt gggtcaatgc ccaaaccttc
     7561 agcctgcgca gtccaacctt cagcatctac ggtgaaataa tttgccggtt gtccttgcac
     7621 attaaatagc gcaacggtta agcaaattat tagtatcaat atcttaaaaa ttttcatata
     7681 tttttattaa acctatatat ttaagctaag ttgttagaag tgttatttta atgtctggct
     7741 tttgttaagt gttttaaatt tatattaaat tgagtatcaa cgaaaaacac ctatagctat
     7801 taattgcaaa gatgagcatg agttgtattt attgtgttac acaactctcc tcctttgtta
     7861 tattattcac acatccaata atttagttca aattttatgg gaaatataag ctgctaatgc
     7921 attgttggga tgcgataaaa tatgttttga ttaatgattt tggaaagaaa agtgcggagg
     7981 cattatattt atcaattttg cataaatggt tgataaaaga taatttcaag caaatgaggg
     8041 tttcggcctt tatccttagt ctagcgaagg ttcgaggttt gaaaattcag taattcgcta
     8101 tgtataaatc aacaataagc ctttatttga ccagaatctc aataaaatca tacaactttc
     8161 ccttttccga ctaattacaa ctggaaattc acaggctcat tttgaaaatg tccggtagta
     8221 tttataaatt ggagaagttg gagttgccgg ttaaaacatt gtcaactact gtaaaatatc
     8281 caatatttaa attgatgata attggacaat gcccaaatat ttcgcttcct atgataaccc
     8341 taattagaaa ggatgctgaa aaatggttga attaccggta ataatttata tctttaacaa
     8401 atgttcagaa ctatccaaaa ttgcttgttt gcatgtgccc taatttttag tttattagtt
     8461 gcctgtaaaa aagaaaaggt tgaggaaata acagcgttta ctccggtacc cacttcagac
     8521 attgttatgt atgaagttaa cattccggca atgagtacca cccgcgatat taatggcgtt
     8581 atagcccgtt tggacatcat taaagaacta ggtattaata ccatttggct tatgccaatt
     8641 tttgagttag gggaattaaa tgcattcggt tcaccatatt gtatcaaaga ttataaagca
     8701 gtaaatccgg aattgggaac ttttgctgat ttacaattat tggttgaaga ggctcatgca
     8761 cgaggtattg ccgttatact tgattgggtt gccaatcata cttcatggga taatgcctgg
     8821 ataacaaatg atggttggta ttcagaagat gcaagtggca atattattag ccctttaggg
     8881 acaacatggt atgatgtagc tgatctgaat tttacgaatg cagatatgcg tcttgccatg
     8941 atagatgcaa tgcaatactg gattgacaat gcgaatattg acggatttag atgcgatgct
     9001 gctgattatg taccttttga tttttggcaa caggcaatta catcgctaaa tgaaaataca
     9061 gaacgtgatt taattttatt agctgaaggc gcacgtgcag atcattttac tgcaggtttt
     9121 gaattaaatt tttcatggag tatgttaggc actctgaaaa atgtttttac aggtggagga
     9181 tatgtaagta ctatcttttc taccaatact gctgaatata tttctgttcc tgatggcagt
     9241 aaaaaattac gttttactac aaatcacgat gaaactaata tcgcaccacc ggctacagct
     9301 tatggtagtc aggatgctgc attggccgct gctgttattg ccttttattt acagggtgtt
     9361 cccttggttt attgcggaca ggaggtaggt atttcaagtg cttctgccta taacggagcg
     9421 cttaattgga caattaatcc ggatatttat caaacctata aggacctgtt aaaaaattat
     9481 aatacctatt cggcgctgcg atatgctttg cctgaaacct ttagcacagc tacagttgcc
     9541 atctttaaaa aacaattaaa cgatgaaaat atggtggtga ttgtaaatac ccgaaacagc
     9601 aatcaaaatg caccggttga tgcgacatta caaggtgagt atgaagatgt tatgaatgca
     9661 aacacattaa ttctcgacga taatattacc ttatcacctt atcagtttta tatattaaaa
     9721 cgtaaataaa aagtaaggca ggaaaaaaat tcctgcccct tatttaatgc tcaataataa
     9781 tttgtttaat aattgtttta tggttgcatg cacttacctg cacataatac atgcctgccg
     9841 gcaaattaaa taccggaatt tttaattgtg cttctgatgt ttgtgaatac catacctgtt
     9901 tgccttgggc agtatatatt gttattgttt taatgtcggc ataattagca caaacgttta
     9961 acatatagga tgaaggattt ggcgaaatat ttatttcttc tacagcagat tgttgcattc
    10021 gcattgttgt gggttctgtt ttaactatcc aggtatcata atcacctaaa ttataaccca
    10081 catctccatt aaaggaattt gtttgtgcca aaaaaacaat actgccatct gctaatacta
    10141 gactttcata ggctttttca ttacggttac caccataaca atgttgccac atcagatgac
    10201 cggctaaatc catattaaac atccacacat ctgtaccttt gtgtttgcca gaaacatccc
    10261 ctgaagtaat agattcagtg tatccggtta cgatataatt gccttcgtta tcagcaacaa
    10321 ttttatcggc aacatcatta ttatttcctc catacacttt tgtatatttt atttcaccta
    10381 aagctgttaa tctggaaaca aatacatcaa tttcaccgtg attacccggg aatgatgaat
    10441 tacttctgcc ggcaactaaa aaatcgcctc ccggagtttt acaaatactt tctgcaatat
    10501 cattattcca tctgcctata gtagtttccc aatataaagc accatcatca ccatctattt
    10561 ttatcaccca tgcatccata gcacctttca cttcagttat gtcaccatta cttgaacttg
    10621 catagccaac tataattatt ttagcataat tagtttctaa tgcttcatgt cccgattcgc
    10681 ttaaagtgcc gccataacat ttggaccaga ttttcccgcc tgagccattt aattttacga
    10741 ctaatatatc agaaacctca gccggtccat gtgaagtaat tgcatctaca tcaattgaat
    10801 tagtactgcc aactgctaaa taattgccat cttgcaaagc aataatatca taggcgtttt
    10861 cagctgcagt tccaccaaaa accgaagacc agactaagtt gccattgcta tccactttag
    10921 ttacccaaaa atcgttgttc ccaatcggtg tacttaaatc accatctgca gaggctgtaa
    10981 cgcctaaaac aatataacca ccgtcatttg cctgcaccac cgattcaaat acatcattct
    11041 cggatcctcc atatgtttta gaccatatga cattgccgtt cgcatcaagt tttatcatcc
    11101 atgcatcata atatccgcca atttcaggtg gcaaatctcc atctgtcgaa ccggaatatc
    11161 caacgccgac atatccgtca ggaatagcaa taaaatcacg gataaagtcc acattgctac
    11221 ccccatacac tttttgccag tcgataaccg gcgcaatttc ctgagctata agaaattggg
    11281 cacataaaat tcctgaaaac attaggtaaa ttcgattttt cataaccttg atttataagg
    11341 caaattaacc ctcaaactac ctggaaacaa tgatgtaggt catatctgta acatatgtta
    11401 ctgtcatatt aattgttgtt aaatgttatg attacattca ccaaagggca gtataattat
    11461 ttttcaaatt cacggttttc cgtgaatgga atttggctcg caggtcttaa tttagtagta
    11521 aattaaaaca gcgcatttta aaatttaagt aattacatca caacaaaata ttcactatgg
    11581 caacgttatt ggtaaagggt agagcattta cagaactgac acgtaaaatt gacccggaat
    11641 taaatgtagc cggcgcctat ccggagaaac aaattttatt ggcaaatgac agagagattg
    11701 gtgctttgca acatgagctt gatattgagg cagacgatgt ttttgaagta gagacaactg
    11761 ataatgttac ctgggtatta acgggtgaag aattattagg aaaatttgca tctactgcaa
    11821 atagaacacg cgcagcaaat aataaatccg gcgatgtgtt tgaacttaat gcatctatta
    11881 ttttcccaac agaagaaaga ggaattggta atgtgattaa tttgcgttcc atcattaaat
    11941 gggcatttaa aagagctatt aaggaaataa aaattattaa tggcttggta gaaattgtcg
    12001 ataaaaaatt agtggaaaat ccgggtttat tccgcgcgga acgtttatta aaaaatgctt
    12061 taaaggaaca gattaaaagc gcaacacaat tatccgatac cgagcccaat ttaattttta
    12121 tacatggcac ggcagccaat gtggaaatga cctacggtaa attaactcca gaaggagatg
    12181 ccgattggct tgaagtgcaa aaacggtttg gtaaacgcat ttatgcttat cagcatcata
    12241 ccataagcaa aagcccttta gataatgcag ttgaattaat agccttgttt cctgatacat
    12301 ttaaactgca tttagttacc tcatctcgcg gtggtttggt tggtgaatta atttttgcta
    12361 cagcctatta caaggacttc ccggtgatgt tggataccct taaaatgcag ttagctgctg
    12421 ctaaagacag agctgatgat gtaaaaaatg tggaacaact tattcaattt ggtaaaacaa
    12481 aaaaaattga gatactggat tataatcgaa tagcttgtcc tgcaaatgga actatattag
    12541 cctcaggtcg attagataaa ttttttctga ttgtactgaa tgcaataaag ttaattccgg
    12601 gcattggtgg taaccctatt tatgaagcaa tttcaacagc attactcaat ttgattaacg
    12661 caaaagctga ttgctcacaa atgccgggat tagaagctat gatgccggaa agtcctttta
    12721 ttaaggcatt aaatagtgcc gatgtagagg tggaccatac tttgaaaata attgccggag
    12781 atactgagcg ctcaaaaatt ttccgtgcaa tggccgtatt gttatctgac atttattatc
    12841 gcacagaaca tgattttatt gtaaatacca attctatgtt ctgcgggtat aaacgtaaaa
    12901 acacgcaata tatttatcac aaaagtggtg ctgttagtca ctttaattat tattacaaca
    12961 atcaaacccg caatccttta tatgccgcat tgaaaggtgt ggagaatatt attgaattta
    13021 gtgttttacc aaatggattg aatttccggt caccttcatt ttctttcact gcttatctcg
    13081 aaaatacacg tggatattat aaaaataaaa ttgtggtaac ccgcgatgaa caggagttgg
    13141 aatttgaaag tgaagcagta atacaaaaat tggatgttaa actcacacat ggagatttag
    13201 gatttgcaga atatccttta attgttgggc attttgaagg tgatggtatt gtaagttctg
    13261 aaaaagctgt agacaaacac atggacaggc gtttattaga aatgcatttg gcgggcattt
    13321 atccgggtga agtagaaaca tctgcagttt tgttaaagga aggtaaagga tttcagggag
    13381 ctgtggtagt tggactggga agttcaggag aactttcggg agaatcgtta gcagcatctg
    13441 ttgctcacgg cgctgtgaat ttggcattta ggtatgcgaa tgcagataaa aaaattcatc
    13501 agattggcat ttctactttg ttgattggtt caggttatgc cggtatatcc atcaaaactt
    13561 ctatcagaag tattattgat ggtatttctc gtgcgaatcg tcgtatcaaa aaaataaata
    13621 atggtgctgt tccatttatt acaaaaattc aatttataga attgtatgag gacagagtaa
    13681 tgatggcagc taaattcatt aacaatacca ttattaatga aacacccgaa aattttgttc
    13741 aaacaaaagt ggaaattgat caggctcccg gttttcaaaa acgattattt gataactcaa
    13801 cgagtgaatg gtggtatcgt ttacaaatat tgactatcaa taacgaaaaa tctgttaata
    13861 aacagttgcg ttatgttttg ttaaacaacg gcgctaaagt ggaagaaatg ctggtatata
    13921 cgcagcgaat tttaatcgat aaaattattg atgctgccat ccatcaacaa aatatcgagt
    13981 tggggttatc gaaattatta tttaatttat tggtgccaaa tagtattaaa gattttactc
    14041 aggagaaacg aaatttgttg ttgattgttg atgctgaaac agcggcatat ccctgggagt
    14101 taatgtacaa tccggtaaaa gaaggcaaca agcctattgc agtaaattca ggaatgatta
    14161 ggcaattaat tgttgaatca ccacataaac cggtattaca tgtagcgggc aacacagcgc
    14221 ttgtaattgg tgatcccaag gtgtcggaag gttggacgca actggaaggt gcacgtgctg
    14281 aagcgcaggc tgtgcaacaa gttttagagc aaggtggtta taaagtaaat gcattaatcg
    14341 acaaagattt tatggcagtg ataagtgcgc tgcacaataa tccgtataaa ataatgcatt
    14401 tcgctggaca tggagatgtt gatttggaag atcctaaaaa ttcagggatg atattagata
    14461 atagtatcag aataacacca agtgaagttg cacagctgga ttatattccg gaattggtgt
    14521 ttattaattg ttgttttttg ggaagtacgc atcaggtaaa agtgcaatgg aatcagttgg
    14581 cagcaaattt aggaacccaa tttattaata taggtgtaaa atgcgtggtt gcagccggtt
    14641 gggcagtaaa cgacgcagct gctgaagaat ttgcaaaaac attttatgat atgatgctta
    14701 ccggatgtga atttggtgag gctgtacagg cggcacggca aaaaatattt gaagatgacc
    14761 gttttagtaa taatacctgg ggagcctacc agtgttatgg tgatccattt tataaattgg
    14821 acccattaag tcacggtaaa tcgggaaaaa gcaataatta tgtcaatata caggaatgta
    14881 tcgtcgcatt gaataatttg attcaacgtt cagaccctcg ttctacccgt agtaaaagca
    14941 cggctgttgt cagtgcaatt aatgcccatg ttgaatcact ggataaatca tggtttaaag
    15001 atgtacagtt attggaagcc attgccgaca gttattatgc aattggtgaa tgggcaactg
    15061 cattaactta ttacgagaaa ttgtatcaaa ttgccacagc gaattttaaa ttagacagcc
    15121 tttaccgttt ttcaaaaatt gccatgaaaa ttggtgtcga aaaggcgaag acaggtaaaa
    15181 ctaaggagga aaaagtaact ggtgaaaatt ggattaaatt ggcatataag attgctgaaa
    15241 atatcaataa cattgctgaa aacgcttata cattgttgtt aattgctggt attaccaaac
    15301 agcatgctta tttatttaaa ggtgtgcaaa gaattaacaa attgaaagca gcagcagcat
    15361 tatatgaaaa agcctttaga gtgcgatacg aagccgggca gggtgctgat tattatccat
    15421 tgcataactg gttaacgatt tatgaattat tgaaatggta taggataaaa ttaaaagtga
    15481 atccacagga aattacccaa tttattcaag atgctaataa tactaaaaaa tctaaaaaaa
    15541 ttaaattaga tttattcagg tatcacagtg ctgaagcaga atttgaaatt tatcagttgt
    15601 tggcggcaac caaacttgct gataaaaaag aaaaaattgc ggagggaatt gtgaaacatt
    15661 ataaaatagg ttgggactat ggcggaacct attataaaac agttgctatt ttggaacaaa
    15721 ttaattttat tgtagaaatg atgaaagaag c
//
LOCUS       DAORKW010000025        15094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_44_length_15094_cov_5.147949, whole genome shotgun sequence.
ACCESSION   DAORKW010000025 DAORKW010000000
VERSION     DAORKW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15094
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44_length_15094_cov_5.147949"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1614
                     /locus_tag="PLB46_02170"
     CDS             274..1614
                     /locus_tag="PLB46_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF75347.1"
                     /translation="MRKLAFIIIISALTTAVQAQRTVTLQECINLATDNNLQLKQGAA
                     GVEFSERAANLSRLLLLPNLNLGSSYFWNFGYTIDPVTNLPLANSFQTNGYQATSTMN
                     LFSGGTISNTIKKTKTDLQVAGMNYKEALENVQFQVIVAYLAVMFAEEQLNIANQKIN
                     TTELQLNNSKKLAQAGSIPEGNLLTIEAQLAQDQLSVIQSQNQLDKTYLDLKLLLQLD
                     PTENIKITFPDISKVDNILNAPVPDALTVANYAIANKASIKKYEYQLLSDGLNKKIAG
                     AAALPTLSLIGQVGTNYSNATYPPIITEADPYGTQINNNLSEVVGISLQIPIFNNGQV
                     LLNKQNADLAIINTQLNQQIAINTMRQNVTQAINDLKAAIASYAAAEKNLAAAQSAFD
                     FAEKKFNMGTASSFDYTNAINIKAQAESTLVQAKYDMIFKSKIIDYYLDKTLDF"
     gene            1625..2923
                     /locus_tag="PLB46_02175"
     CDS             1625..2923
                     /locus_tag="PLB46_02175"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF75348.1"
                     /translation="MRSLKILGIIIAVLIVVLVIGSNKGWFNGGAKITVNAEAVQKRT
                     IVETVTASGKIYPVTQVSISAEISGEIVDLPVKEGDKVKEGDLLMRINPDLYESQVEQ
                     AQAGLDNTKAQLATARARVLQSKLQFDNAKITFDRNTQLLKDKVISQMDYEQAQLAYE
                     TSKAEYEIAQESVTAMEFTVKSSEAMLREMRNNYKRTSIYAPAAGTVVGLNKKKGEKV
                     LGTIQMTGDQLMSIADLTQMEIQVDVSENDVLRIDVGDTANIEVDAYLNRKFKGVVFQ
                     IANSAGSGSVLSAVSDQATNFKVKIYLLPESYADLMKESNGKYPFYPGMSATVEIKTD
                     VQRNVLSIPIQAITTREDTTDTNKDKKINEIVYVLDGEKAKEVIVTSGLQDDNFIQIK
                     TGLNEGEKVISGPYNTITNVLKDGDMVKVEEDKDEKEDKK"
     gene            3088..3615
                     /locus_tag="PLB46_02180"
     CDS             3088..3615
                     /locus_tag="PLB46_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRF75349.1"
                     /translation="MAQVENTRSAAVGKHNSRTSRKSLFIDLTPMVDLAFLLISFFML
                     TTVLQENMSMELKMPENTGEPMPIPHSRTITLIADANNQLYYYNGEDMQNLAKSDYSN
                     AGLRQLLYERIKQQRAADPDKGLICIIKLTEGADYNDMINLLDEMEITDVPTYAIQDI
                     TEAEKEQLNIFTKNN"
     gene            3670..4464
                     /locus_tag="PLB46_02185"
     CDS             3670..4464
                     /locus_tag="PLB46_02185"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF75350.1"
                     /translation="MNNLKNYSDASIDDMVFENRNQSYGAYELRRSSDKHLKIALFIG
                     ILSTSVFTAFSLNNKKMEVIDEPTIIVTTVSDLPDIPELPKIEPPQPIVQEVIQQASV
                     AFTEMIVREDLEVTEEVIAIEDLKGKVISNKTVEGPKIEVPIIKDPIITEPVVTRQEP
                     ITIAEQMPEYNGGFASMQRDIQKQVRYPSQAMRMGTEGTVYVSFVVNTDGSVSDIALV
                     RGIGDGCDEEAMEAVSKMQKWNPGKQNGKTVRVKITVPIKFELEND"
     gene            complement(4554..5243)
                     /gene="rnc"
                     /locus_tag="PLB46_02190"
     CDS             complement(4554..5243)
                     /gene="rnc"
                     /locus_tag="PLB46_02190"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRF75351.1"
                     /translation="MGPEKVFCRKLRSITGLAPINYNVYKLAFQHSSIHNSVHTNNER
                     LELLGDAVIDLVIADFLFKKYPYKTEGFLTEMRSKIVSREQLNRVALKIGLGEMMQIR
                     KGGTDLTKSNVLGNALEALVGAIYVDTGYTRASRFFMHKILIPHYEIAELESVVVNFK
                     SKIIEWAQKNGRDIKFDLIEEHELKGKRMYKMGLFLDTELIAEDIDYSKKRAEQKASE
                     IGCKKLGIHEV"
     gene            complement(5284..6537)
                     /gene="fabF"
                     /locus_tag="PLB46_02195"
     CDS             complement(5284..6537)
                     /gene="fabF"
                     /locus_tag="PLB46_02195"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRF75352.1"
                     /translation="MASRRVVVTGLGALTPLGNSVPEYWEGLVAGKSGADFITRFDAT
                     NFKTRFACEVKNFDPTSVIEKKEVRRMDPFLHYAIAAVEEAVQDAGIKSEEVNVDRVG
                     VIWASGIGGIKTFEEEVFAFAKGDGTPRFNPFFIPKMISDIAAGMISIRYGFRGVNFA
                     TVSACASSTHSIIIAADCIRMGRADIIISGGSEASVTAPGVGGFNAMKALSERNDSPQ
                     TASRPFDLDRDGFVLGEGAGALILEEYEHAVKRGAKIYAEYMGGGMSADAYHMTAPHP
                     DGLGAQLVMKDALRDANMQLDELDYINVHGTSTPIGDPQEIKAIQTVFGEHAYKMNIS
                     STKSMTGHLLGAAGAIEAIASVLALNNGIIPPTINHFTDDPVFDTRLNLTFNKAQYRE
                     LNAVLSNTFGFGGHNASVIFKKLSS"
     gene            complement(6552..6788)
                     /locus_tag="PLB46_02200"
     CDS             complement(6552..6788)
                     /locus_tag="PLB46_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRF75353.1"
                     /translation="MSEIAEKVKKIIIDKLGVDESEVVPEASFTNDLGADSLDTVELI
                     MEFEKEFNISIPDEQAEGITTVGDAIAYLEEHVK"
     gene            6930..7379
                     /locus_tag="PLB46_02205"
     CDS             6930..7379
                     /locus_tag="PLB46_02205"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HRF75354.1"
                     /translation="MARQKIKIDPEFNFILIGIATPLQDYRMAWFINNILYKQLAKID
                     DLIVADPVNRVQTAYGRFDYVEELTKSVFHLVQNKQGANNLIPELKELDYLLLIKGEY
                     YRPRKNEIVKKIRRIEQILAVAIIDVTTLKSKNNLIFEDTFKKKNQV"
     gene            7405..8832
                     /gene="pyk"
                     /locus_tag="PLB46_02210"
     CDS             7405..8832
                     /gene="pyk"
                     /locus_tag="PLB46_02210"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRF75355.1"
                     /translation="MKRGGNKTKIIATLGPASNNFEMIVKLINTGVDVFRLNFSHGSH
                     EIHKHTIELIKRANKELNTHVSILADLQGPKIRLGEVLPLTVLKEGDTFEFTTSPMIG
                     NNKIAYITYPNFPSDVRPGETILIDDGKIELKVLETNQKNHVITKVVFGGPISSKKGV
                     NLPDTQVSIPSLTEKDHADLLFALEHGANWIALSFVRKADDIIHLKNIIRHHGKHSKV
                     IAKIEKPEALKNIDEIIDATDAIMVARGDLGVEMPVELVPFTQKDLCRRCLEKTTPVI
                     IATQMMESMIENAHPTRAEVTDVANAVLDGADAVMLSAETAAGKYPEKVISTMVRIIE
                     QAEKNEIVYNRRHQPDKHSKTYLSDAICFNACNIADEVHAAAIMGMTKTGYTAFMVSS
                     CRPKAKIYVFTNNEEVVNILNLLWGVEAFYYDGMRGTNNTFKEVQQHLAHIGYLKKDD
                     VVINLGTMPVKEMARTNTLKISVIQ"
     gene            8946..9203
                     /locus_tag="PLB46_02215"
     CDS             8946..9203
                     /locus_tag="PLB46_02215"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HRF75356.1"
                     /translation="MLVSYTKYMWKEENNTLSKTFKFANFIDAFAFMTRVALLAEKQN
                     HHPNWSNVYNTVTINLNTHDAGDVVTEKDRKLAEAIDKLVA"
     gene            9278..11392
                     /gene="ligA"
                     /locus_tag="PLB46_02220"
     CDS             9278..11392
                     /gene="ligA"
                     /locus_tag="PLB46_02220"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRF75357.1"
                     /translation="MKYSAENQQELQQLTSTLIKNIADANYTSDAARDVIDELKQVIN
                     FHDWKYYVEAQPTIKDYDYDQLFKKLKSLETTFPELLTPDSPTERVARTLTSDFPTVA
                     HSIPMLSLENSYDENDLNDFDKRIKELTGRTAIEYCVEPKFDGSSIAIIYENDLLVRA
                     ATRGDGIQGDEITNNARRMKSVPLSAKFSALGIHRVELRGEVVINKKTFAKINEKREE
                     EGLQILQNPRNSAAGALRVKDSIEVEKRGLEAFMYQMAYAVDKNGNDMFNTTLLNKHF
                     NNINQLGTLGFKIPNQEKKLCKSIAEVHAFIQSWEEKRDDYEYEIDGMVIKVNDIDLQ
                     KECGATSHHPRWAIAYKFKAREVETELLDVEYQVGRTGAITPVAKVKPVFVGGVTVSS
                     ISLHNEDIIREKDIRIGDVVVVQRAGDVIPYIDRVVVEKRKSDSTPFDFTRECPSCHQ
                     PIVKPAEEAVYRCVNAECEAQAEERLIHFVSKDAMDIDGFGRETVSSFYTEGLLRSIP
                     DIYKLDFESVGKLEGWKEKSINKLRDGIAKSKEQPLWRLVNGLGVRHIGTQTAKDLVK
                     TLNNLKELFEVTPEQLQAIDGIGPKVASSVLDYFHNAGNQHMILELEASGLNLKNQKV
                     EAQSAKLNNLTFLFTGSLTRFTRDEAKELVEGNGGKLISAVSKNLNYLVAGENAGSKL
                     DKAKALGTVNIIDEEAFLKMLE"
     gene            11544..11795
                     /gene="rpsT"
                     /locus_tag="PLB46_02225"
     CDS             11544..11795
                     /gene="rpsT"
                     /locus_tag="PLB46_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRF75358.1"
                     /translation="MAHHKAAKKSIRQAEKRNESNRYYGKTTRNAIRKLRAAEPAEAD
                     KMLPDVLSMIDKLAKRNVIHKNKAGNLKSALMKKPVAAK"
     gene            11877..12572
                     /gene="radC"
                     /locus_tag="PLB46_02230"
     CDS             11877..12572
                     /gene="radC"
                     /locus_tag="PLB46_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRF75359.1"
                     /translation="MEENTGFTLKQLAEEDRPREKLQLKGRQSLTNAELLAILIGSGN
                     AKETAVELSQRILLHYRNNLDVLGRLTIDDLLKFSGIGPAKAITIIAALELGRRRNLA
                     EAEQKVQIKSSRDIYEVMYPLIADLPNEEFWVLHLNKANRIIEKERISIGGIGGTVVD
                     VKIILKSALQKLASAMILVHNHPSGNLTPSDADLSITKKLRDAATYLEILVLDHVIIG
                     DKNYYSFADNGNI"
     gene            12588..13499
                     /locus_tag="PLB46_02235"
     CDS             12588..13499
                     /locus_tag="PLB46_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRF75360.1"
                     /translation="MNQEYKKAYLLYHVCVFLWGFTAVFGKIIDLQETILVWWRMGIT
                     SLILLCIPALWKTIRNINKKTLLQLAGIGVLVTCHWISFYGSIKASNISVALTCLATT
                     SFFVSVFEPLMFNKKFIWYELILAVLVIPAIYLIFHFSGSYTEGIILGLASTIFATLF
                     SLLNKKMISKTAPLNITFIEIAVGFILLSVVLPFYMQTQEDIKFWPTTMDTVNLILFS
                     ALCTVVPFTLSLYCMRHISVFTASITLNLEPVYGIIMAIVFFNENEYLAPGFYIGTLL
                     ILLVVFINPFIQRRFGGPIKPPVTIGE"
     gene            13601..>15094
                     /locus_tag="PLB46_02240"
     CDS             13601..>15094
                     /locus_tag="PLB46_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF75361.1"
                     /translation="MSKQSADFELFFRISIDAMIIADLDGKILDFNHAYEIITGYTRD
                     ELFELNGGWGLILDEDIPALRQKIDELKTGASVMKNYEVRLVSKSGEIKTISYDAKVD
                     FINEQIYAIGRDITIIKEQEKSLKLNESRLAFFFDNAFDGMVIILDGKYHLVNKAFLQ
                     ILGLQHADDVIGKSYLDFVHESSKEKAQSIISDDSEALYELDIVRADGSLRNIEIAGK
                     SLIFNNQRMRIAVIRDLDDRRNMLQKAQENEDRFRAIFQNSTMGIVLTEFDGTILEIN
                     QTLAEQLGYSVAELMGGSVLDLIYEPDKPAALKGATMLYEGKVDISYAERRLIKKTNS
                     ITWTRMTCSVITTTGNRKMVLGILENIDNQKHNEKALIESEEKFRAVYESSPMGILIT
                     KNPGVIVDVNPAFAEMIGFQFYNRNIIANTNKYNFKMEEYFHLHEKILFSTIAQRKKI
                     KGLVKMRVDMIVLATICTAVVLRETGIKKMSLSKYALKEGALSAVIAN"
BASE COUNT         5035 a         2647 c         2984 g         4428 t
ORIGIN      
        1 gggccttttc cgccattttt agggcttctt ccatgaaaaa tgcatccgga tctgatataa
       61 ttcccatgcc gtaaaggtaa gggcttaatc gaatagactt caaatttttg acttgaaaac
      121 tcgtctatca acttgtttaa ggttaaatgt gttaatagaa atactgtttt tcgcttgtaa
      181 cttttaatgc ttttggacca ttcatcctta aaatgtctta atttatctaa aactttaaaa
      241 ggctcgtcaa atttgaacta attttaaccc gtcatgagaa aactagcttt tatcattatc
      301 atttctgccc taactacggc tgtgcaggct caacgcacag ttactttgca ggaatgtatc
      361 aacctggcta ccgacaataa cctccagtta aaacaaggtg ccgcaggtgt tgaattttct
      421 gaaagagcag ctaacctaag ccgattgctg ttattgccca atctaaacct tggaagttcc
      481 tatttctgga atttcgggta taccatcgac cctgttacca acttgccttt ggctaatagt
      541 tttcagacaa atggttatca ggctacctct actatgaatt tatttagtgg cggtaccatc
      601 agcaatacca ttaaaaaaac taaaaccgat ttgcaggtgg caggaatgaa ttataaagaa
      661 gcattagaaa atgtgcagtt ccaggttata gttgcctatt tggcagtaat gtttgctgaa
      721 gaacaattga atatcgccaa ccaaaaaatt aatacgacag aacttcagtt aaataattca
      781 aaaaaattgg cgcaggccgg aagtatccct gaaggtaatt tacttacaat tgaagcacaa
      841 ttagctcaag accaactttc cgttatacag tcgcaaaatc aattggataa aacctatctc
      901 gatttaaaat tattattaca attagaccca acagaaaata ttaaaattac ttttccggat
      961 atttccaaag tggataatat tttaaatgca ccggttcccg atgcacttac tgttgctaat
     1021 tatgccattg ctaataaggc cagtataaaa aaatatgaat atcaactatt gagcgacgga
     1081 ttaaataaaa aaattgccgg tgctgcagcg ctgccaactt tgagtttgat tggacaagta
     1141 ggaacaaatt attcaaatgc aacttatccg ccaattatta cagaagcaga cccatacggc
     1201 acacaaatta ataataacct aagtgaggtt gttggtattt cattacaaat tccaattttt
     1261 aacaacggac aagtattgct taacaaacaa aatgcagatt tagctattat caatacccaa
     1321 ttaaatcagc aaattgccat taataccatg cgccaaaatg taacgcaggc aattaatgat
     1381 ttaaaagcag caattgcatc ttatgctgcc gcagaaaaaa atcttgcagc agcacaaagt
     1441 gcattcgatt ttgcagaaaa gaaatttaat atgggaacgg cttcttcgtt cgattatacc
     1501 aatgcaataa atattaaagc acaagctgaa tcaacacttg tgcaggctaa atacgatatg
     1561 attttcaaat cgaaaattat tgattattat ctcgacaaaa cattagactt ttaaacaacc
     1621 aattatgcgc tcgttaaaaa ttttaggaat tataatcgct gtattaatcg ttgtgctggt
     1681 aatcggctca aataaaggat ggtttaatgg cggtgctaaa ataaccgtca atgctgaagc
     1741 agttcaaaaa agaactattg tagaaactgt tactgccagc ggaaaaatat atcctgtaac
     1801 tcaggtgagc atttctgccg aaatttcagg tgaaatagtt gatttgccgg tgaaagaagg
     1861 tgataaagtg aaagaaggtg atttgctcat gcgtatcaat cccgatttat atgaaagtca
     1921 ggtagaacag gcacaggcag gtctcgataa tacaaaagcg caattagcaa cagcaagagc
     1981 gcgcgtttta caatcgaaat tacaatttga taatgcaaaa attacattcg acagaaatac
     2041 gcaattatta aaagataaag tgatttctca aatggattat gagcaagcac aattagctta
     2101 tgaaacatca aaagcagaat atgaaattgc tcaggaaagt gtaacggcaa tggaatttac
     2161 ggttaaaagc agtgaagcca tgttacgtga aatgcgcaat aactacaaac gcacatctat
     2221 ttatgcacct gcagcaggaa ctgttgttgg tttaaataaa aagaaaggtg aaaaagtatt
     2281 aggtactatt caaatgaccg gtgatcagtt aatgagtatt gccgatttaa cacaaatgga
     2341 aattcaggtt gatgtaagtg aaaacgacgt gttgcgtata gatgttggtg atactgctaa
     2401 tatagaagtg gatgcttatt taaacagaaa attcaaaggc gtagtttttc aaattgccaa
     2461 ttctgccgga agtggaagtg tattaagcgc agtgtctgac caggcaacta attttaaagt
     2521 gaaaatttat ttattgccgg aatcgtatgc tgatttaatg aaagaatcaa atggcaaata
     2581 tccattttat ccgggtatga gtgcaacggt tgaaattaaa actgatgtac aacgtaacgt
     2641 attatctata ccaattcagg caattactac tagagaagat actaccgata caaataagga
     2701 taaaaaaata aatgaaattg tatatgtact tgatggcgaa aaagcgaaag aagttatcgt
     2761 aacatcagga ctgcaggatg ataattttat ccaaattaaa actggattaa acgaaggcga
     2821 aaaagttatt tcaggtccat acaataccat taccaacgta ttaaaagatg gtgatatggt
     2881 taaagtggaa gaggataaag acgaaaaaga ggataagaaa taggtggttg atgatttaat
     2941 caaacccaat tatttaatgt gttttttata aaggtgctgt cgcgatgtaa ttaatcgcac
     3001 tgtcatggta ttctcatttt catgagaatt gtaaaaccct ttatggaatt gtgcgtatac
     3061 aacatcataa cagtaaaaca tattgttatg gcacaagtag aaaacacccg ttctgccgct
     3121 gttggaaaac acaatagccg cacttcccga aaatcattat ttattgattt aacaccaatg
     3181 gttgatttgg catttctgct gatatcattt tttatgctta caactgtatt gcaggaaaat
     3241 atgtcgatgg aattaaaaat gccggaaaat accggtgaac ctatgccaat tccccactcc
     3301 cgaactatta cgcttattgc tgatgcgaat aatcaactct attattataa tggagaggat
     3361 atgcaaaacc ttgcgaaaag tgattactcc aatgccggat tacgacaatt attatacgag
     3421 cgtataaagc aacaacgtgc agctgatcct gacaaaggac tgatatgtat tatcaaactt
     3481 accgaaggtg ctgattacaa tgatatgatt aatctgttag atgaaatgga aattaccgat
     3541 gtgcccacct atgccattca ggatattacc gaagccgaaa aagaacaatt aaacattttt
     3601 acaaaaaata attaagcagt gcttatggaa aaatgaatac cctttcatca caatctaaaa
     3661 ttcactgcta tgaacaattt aaaaaactat tcggatgcca gtatcgatga tatggttttc
     3721 gaaaaccgca accaatctta tggcgcttac gaattgcgca ggagttctga caaacacttg
     3781 aaaattgcat tgtttatcgg tattctcagt acatcagttt tcactgcatt tagtttaaac
     3841 aacaaaaaaa tggaagtgat tgatgagcct acaataattg tcaccactgt atccgatttg
     3901 cctgatatac cggagttacc gaaaattgaa ccaccacaac ctattgtgca ggaagtgata
     3961 caacaagctt ccgtagcttt taccgaaatg attgtaagag aagatttaga agtaactgaa
     4021 gaagtaattg ctattgaaga cttaaaaggc aaagtaatca gcaataaaac tgttgaagga
     4081 cctaaaattg aagtgccaat aattaaagat cctattatta ctgagccggt agtaacccgt
     4141 caggagccta ttacaattgc cgaacaaatg ccggaataca atggcggatt tgcatccatg
     4201 caacgcgata ttcaaaaaca agtacgttac ccatcacagg caatgcgtat gggaactgaa
     4261 ggaacagtgt atgtaagttt tgttgtaaat accgatggtt ctgtttccga tatcgcttta
     4321 gtgcgcggaa ttggtgatgg atgtgatgag gaagccatgg aagctgtatc caaaatgcaa
     4381 aaatggaatc cgggaaaaca aaatggtaaa acagtaagag tgaaaattac tgttcctata
     4441 aaattcgaat tagaaaatga ttaagtacaa ttgagttaac aaatcaggtt cgcctcccgt
     4501 tacttttcgc gggaggtttt ttattttata ggatgtagtt gggcggttga agattaaact
     4561 tcatggatgc caagtttttt acaaccaatt tcactggctt tttgctcggc gcgtttttta
     4621 ctgtagtcga tatcttctgc aataagttcg gtatctaaaa acaatcccat tttatacatg
     4681 cgtttgccct ttagttcatg ttcttcaatc aaatcgaatt tgatatcacg accatttttt
     4741 tgagcccact caataatttt acttttgaaa tttaccacta cactttctaa ctcggcaatt
     4801 tcgtagtgag ggattaaaat tttatgcata aaaaacctgc tggcacgggt ataaccggtg
     4861 tcaacataaa tagctccgac taatgcttcc agtgcattac ccagtacatt acttttggta
     4921 aggtcggtgc cgcctttgcg gatttgcatc atttctccga ggccgatttt aagggcaacg
     4981 cggtttaatt gttcgcgact gacaatttta ctgcgcatct cggtaaggaa accttctgtt
     5041 ttgtaaggat attttttaaa aaggaagtct gcaatcacca aatcaataac ggcatcacct
     5101 aataattcga ggcgttcgtt attggtatgc actgaattgt ggatggaact atgctgaaaa
     5161 gccagtttgt agacattata attaatagga gccaggccgg taatactgcg tagtttacgg
     5221 caaaagacct tttcaggtcc catattacaa ttataaaatt tgcgaatttc cgacagcaag
     5281 caattaacta ctcaactttt taaagataac agaagcatta tgtccgccaa atccgaaggt
     5341 attactaagg acagcgttca attcacgata ttgtgcttta ttgaatgtta agttaagtct
     5401 ggtatcgaaa acagggtcgt cggtgaagtg gttaatggta ggaggaataa taccattatt
     5461 taaagcaagc acgctggcaa tagcctcaat agcgcctgct gcacctaata agtgacctgt
     5521 cattgatttg gttgagctga tattcatttt ataggcatgc tcaccaaaaa cagtttgaat
     5581 agcctttatt tcctgagggt cgccaatagg ggtagaagtg ccgtgaacat tgatataatc
     5641 gagctcatcg agttgcatat tggcatctct taaagcatct ttcataacca actgagcacc
     5701 aaggccgtca ggatgaggcg ctgtcatatg ataagcatcg gcactcatgc ctccgcccat
     5761 atattcagca taaattttag ctccgcgttt tacagcgtgt tcgtattctt ccaaaataag
     5821 tgcgccggcg ccttcaccaa gaacaaaacc atcgcggtcc aaatcgaaag gacgagatgc
     5881 agtttgaggt gagtcgttgc gttcacttaa ggctttcatg gcgttaaaac caccaactcc
     5941 cggagcagta acagaagctt ctgaaccacc ggaaataata atatcagcgc gacccatacg
     6001 aatacagtcc gcagcaataa ttattgagtg tgttgaggat gcacaggcag ataccgttgc
     6061 gaagtttacg cctctgaaac catacctgat ggaaatcata ccggcagcaa tatcgctaat
     6121 catcttagga atgaaaaatg ggttgaaacg cggcgttcca tcacccttgg caaaggcaaa
     6181 aacctcttct tcaaaggttt ttataccacc aataccactg gcccagatta caccaacgcg
     6241 gtcaacatta acttcctcag acttaatacc tgcatcttgc acggcttctt caactgctgc
     6301 aatagcataa tgaagaaacg gatccatacg gcgtacctct tttttctcga taacgcttgt
     6361 aggatcgaag tttttcacct cgcatgcaaa gcgggttttg aagttggtag cgtcaaaacg
     6421 tgtgatgaaa tcagcaccgc tcttgccggc taccaacccc tcccaatact ctggtacaga
     6481 gttgcccagg ggggtcaatg cccctaaacc ggttactaca actcttcttg atgccataag
     6541 aaggataaga attacttaac gtgttcttcg aggtaagcaa tagcgtcacc aactgtggta
     6601 atgccttcag cctgctcatc aggaatagag atattgaatt ctttttcaaa ttccataatt
     6661 aactctactg tgtctaatga atcagcgcct aaatcattgg taaagctggc ttccggaact
     6721 acttcgctct cgtcaacacc aagcttgtcg ataatgatct tttttacttt ttctgcaatt
     6781 tctgacattg ttttaggttt taatactccc ttcggaggga ggaattgttt gaaaataaag
     6841 tgcaaagata agggaatgta tatggattgt gcaagtgtgc agagatataa tagttgtatc
     6901 ttacctttga ggcatcattt tttttcactt tggccaggca aaaaatcaaa attgaccccg
     6961 aatttaactt tattcttata gggatagcta ccccccttca ggactatcgt atggcctggt
     7021 ttattaataa tatactctat aaacaactgg ctaaaattga cgatttaatc gtcgcagacc
     7081 ccgtaaacag ggtgcaaacc gcctatgggc gcttcgatta tgtagaagaa ctcactaagt
     7141 cggttttcca tctggttcaa aacaaacagg gggcaaataa cctcattccc gagctaaagg
     7201 agctagacta cctcctgttg ataaagggag agtattatcg accaagaaaa aatgaaattg
     7261 taaaaaaaat tcgcaggatt gaacaaattt tggctgtagc cattattgat gtgactactt
     7321 taaagtcgaa aaacaacctc atctttgagg atacattcaa aaaaaagaat caggtataac
     7381 caaacaacag gatattcaaa tacaatgaaa cgaggaggaa acaaaactaa aattattgca
     7441 acattaggac cagccagcaa taattttgag atgattgtaa agctgataaa cacgggcgtt
     7501 gatgttttca gattaaattt ttcacatggc agtcacgaaa ttcataaaca tactattgag
     7561 ctcatcaaaa gagcgaataa agagctcaat acgcatgtga gtattttggc cgacttacaa
     7621 ggcccgaaaa ttcgtttggg ggaggtgttg ccacttactg ttttaaaaga aggtgacaca
     7681 tttgaattta ctacttcacc aatgataggt aataataaga tagcctatat tacttatcct
     7741 aattttccaa gtgatgttcg tcccggtgaa actattttga ttgatgatgg aaaaattgaa
     7801 cttaaagtat tggagacaaa tcaaaaaaac catgtaatta ccaaagtggt ttttggtggt
     7861 ccgatatctt caaaaaaagg ggtgaattta ccggatactc aggtttcaat tccttcgctt
     7921 acagaaaaag atcatgccga tttattgttt gcattggaac atggtgccaa ttggattgca
     7981 ctttcttttg tgcgtaaagc tgatgatatt attcatttaa aaaatattat ccgtcaccat
     8041 ggcaaacact ctaaagtaat tgccaaaatt gaaaaacctg aagccttaaa aaatatcgat
     8101 gaaataattg atgcaaccga tgccattatg gtagccagag gcgatttagg tgttgagatg
     8161 ccggttgaac ttgtgccttt tacccaaaaa gatttgtgtc gccgttgcct tgaaaaaaca
     8221 acgccggtaa ttattgctac acaaatgatg gaaagtatga ttgaaaatgc ccatccaacc
     8281 cgagcagaag ttaccgatgt agccaatgcc gtactcgatg gtgcagatgc agttatgtta
     8341 agtgccgaaa ctgctgcagg gaaatatcct gaaaaagtaa tttcaactat ggtgcgtatt
     8401 attgagcaag cagaaaaaaa tgaaattgta tataatcgcc gccatcagcc ggacaaacat
     8461 tctaaaacgt atttgagtga tgccatttgt tttaatgcct gcaacatagc cgatgaggtg
     8521 cacgctgcgg ccattatggg tatgacaaaa acaggataca ctgcctttat ggtatcaagc
     8581 tgcagaccga aagcgaaaat ttatgtattt accaataatg aagaagtggt aaatattctc
     8641 aatttattat ggggagttga agcattttat tacgacggca tgaggggaac aaataataca
     8701 tttaaagaag tgcaacaaca tcttgcacat attggatatt tgaaaaaaga tgatgttgtt
     8761 atcaacttgg gaacaatgcc tgttaaagaa atggcaagaa ccaacacctt aaaaataagt
     8821 gtaatccaat aattttattt aataagacat tacatattga agtgtggcaa tcacattgtg
     8881 ccacacttca acctcttccc tccccgtaac ttcccttctt cccgccttcc cggcaaaaaa
     8941 aacgtatctt agtatcttat actaaatata tgtggaaaga agaaaataac accctctcaa
     9001 aaacattcaa attcgccaat tttattgatg catttgcatt tatgacacgc gtagctttac
     9061 tcgccgaaaa acaaaaccat catcctaact ggagtaatgt atataatacc gttaccatca
     9121 atttaaatac acatgatgcg ggagatgtag tgaccgaaaa ggacaggaag ctggctgagg
     9181 cgattgataa acttgtggcg taattaaata ataattattc gggtgtatca tttattttga
     9241 aaataatctc tttaatttgc aataattaat tagggatatg aaatacagtg cggaaaatca
     9301 acaggaatta cagcaactca catcaacact aataaaaaat attgcagatg cgaattacac
     9361 ttctgatgct gcacgtgatg tgatagatga attaaaacag gtaatcaact ttcacgactg
     9421 gaaatattat gttgaggcgc aaccaacaat aaaagattac gactacgatc agttatttaa
     9481 aaaactgaaa tcgcttgaaa ctacttttcc ggaattgttg acacccgatt ctccgactga
     9541 acgtgttgct cgaacactta cgagcgattt tccaactgtt gcacattcta ttccgatgtt
     9601 aagtctggaa aatagttatg acgaaaacga tttaaacgat tttgataaac gtataaaaga
     9661 attaacagga cgtacagcaa ttgaatattg tgttgaaccg aaatttgatg gttcatctat
     9721 tgctataatt tatgaaaacg atttattagt tcgcgcagca accagaggtg atggtattca
     9781 gggtgatgaa attaccaata atgcacgtcg catgaaatcg gtgccactta gtgcaaaatt
     9841 ttctgcattg ggtattcatc gtgtagagct gaggggagag gtggtgatta ataaaaaaac
     9901 ctttgcaaaa attaatgaaa aacgtgagga ggagggttta caaatcctcc agaacccgcg
     9961 taattcggca gcaggagcat tgcgtgtcaa agattctatt gaagtagaaa aacgtggcct
    10021 cgaagcattt atgtatcaaa tggcttatgc tgttgataag aatggtaacg atatgtttaa
    10081 tacaaccttg ttgaataaac attttaataa tattaatcaa cttggaacac tcggatttaa
    10141 aatcccgaat caggaaaaaa aattatgtaa atcaattgca gaagtacatg catttattca
    10201 gtcgtgggaa gaaaaacgcg acgattatga atatgaaata gatggcatgg taattaaagt
    10261 aaatgatatt gatttgcaaa aagaatgtgg tgcaacatca catcatccac gctgggcaat
    10321 tgcttataaa tttaaagcaa gagaagtaga aacggaatta ttagatgtag aatatcaggt
    10381 tggtcgcaca ggcgcaatta cacccgttgc aaaagtgaaa ccggtatttg ttggtggtgt
    10441 aactgtttca tctatttctt tacataacga agatattatt cgagaaaaag atattcgtat
    10501 tggtgatgta gtagttgtac aaagagcagg tgatgtaatt ccatacattg accgtgttgt
    10561 tgtagaaaaa agaaaatcag atagtacacc atttgatttt acccgcgaat gtccatcttg
    10621 ccaccaacct attgttaaac ctgccgaaga agcggtttac cgttgtgtaa atgcagaatg
    10681 tgaggcacaa gctgaagagc ggttaataca ttttgtaagt aaagatgcta tggatatcga
    10741 cggatttggt cgtgaaaccg tgagctcatt ttataccgaa ggattgttgc gttccattcc
    10801 ggatatctat aaactggatt ttgaaagcgt tggcaaactg gaagggtgga aagaaaaaag
    10861 tattaataag ctgcgcgatg gtattgcgaa aagtaaggag cagccactgt ggcgcctggt
    10921 aaacgggttg ggtgttcggc acatcggtac acaaacggcc aaagaccttg taaaaacgct
    10981 taacaacctc aaggagctgt ttgaggtaac tcctgagcag ttgcaggcta tcgatggcat
    11041 cggtcctaaa gtagcctcta gtgtgctgga ttattttcat aatgccggaa accagcacat
    11101 gattcttgaa ctggaagcca gcggtttgaa cctgaaaaat caaaaagttg aagcccagtc
    11161 tgcgaaactc aataacctta cctttttatt caccggttcc ctcacccgat ttacccgaga
    11221 tgaggccaag gaactggtgg aaggcaatgg tggcaagctg attagcgctg tctccaaaaa
    11281 ccttaattac ctggttgccg gcgaaaatgc aggctccaaa ctagataaag caaaggcctt
    11341 aggaacggta aatattattg atgaggaagc ctttttgaag atgctagagt agttggaaac
    11401 cgcacagatt gtgggttttg ggtaaaaatt ccggttcttg gctatcaacc accccaaaac
    11461 aggcaaaata tgtctggtat ttctttggtt tttacgtaag tcgccgtata tttgcactcc
    11521 aatttaaaaa ctaagataat tgtatggcac atcataaagc cgctaaaaaa tcgattcgtc
    11581 aggcagaaaa acgtaatgag tctaaccgtt actacggaaa aactacacgt aatgcaatca
    11641 gaaaactcag agcagcagaa cctgcagaag cagataaaat gttacctgat gtactttcta
    11701 tgattgacaa attggctaaa agaaacgtaa ttcataaaaa taaagcaggc aacttgaaat
    11761 cagctttgat gaaaaagccg gtagctgcga aataatattt ttattcagat attttgaacc
    11821 tgataggatt tcctatcagg tttttttatt tttgggactt gtgaggagga tttgtgatgg
    11881 aagaaaatac tggctttacc ctaaagcagc ttgccgaaga ggaccgtccg cgtgagaagc
    11941 tgcaattaaa aggacgacaa tcgttaacga atgctgaact gctggccatt ttaattggca
    12001 gtggcaatgc aaaagaaact gcagttgaac tgtcccaacg aatattatta cactatcgta
    12061 ataacctgga tgttttaggt aggttaacaa ttgatgattt gttaaaattt agcggaattg
    12121 gtcctgcaaa agcaataacc attattgctg cgcttgagtt gggtcgaaga agaaatctag
    12181 cagaagctga gcagaaagtg cagattaaat cgagtcgtga tatttatgaa gtaatgtatc
    12241 cgcttattgc cgatttgccc aatgaagaat tttgggtatt acatctcaac aaagccaatc
    12301 gaattattga aaaggaacgc attagcattg gcggcattgg tgggacagta gtggatgtga
    12361 aaattatttt gaaaagcgca ttacaaaaac tggcttcggc tatgatttta gtgcataatc
    12421 acccttcagg caatttgacg cctagcgatg ccgatttatc tatcactaaa aaattacgtg
    12481 atgcagcaac atatttagaa attttggtgc ttgaccatgt aattattggc gacaagaatt
    12541 attacagttt tgccgacaac ggtaatattt aattttacct ttgttgtatg aatcaggaat
    12601 acaaaaaggc atacctgctg taccatgttt gtgtgttttt atggggattt actgctgtgt
    12661 ttggtaaaat tatcgaccta caggaaacca ttttagtttg gtggcgtatg ggtattactt
    12721 cactcatttt attgtgtatt cctgcattat ggaaaacaat acgcaacatc aacaaaaaaa
    12781 cattgttgca attagcaggc attggtgttt tagttacctg ccattggatt tctttttatg
    12841 gttctataaa agcatctaat atttcagtag cattaacttg tttagcaaca acctcttttt
    12901 ttgtttctgt ttttgaacca ttaatgttta ataaaaaatt tatttggtac gaattaattc
    12961 ttgctgtatt agttatccct gctatttatt taatttttca tttttccggc tcttatactg
    13021 aaggtattat tttaggattg gcatcaacca tatttgctac gttgttttca ttactgaata
    13081 aaaaaatgat aagtaaaaca gctccgttaa atatcacatt tatcgaaatt gcagttggct
    13141 ttattttgct ttcagtggtg ttgccgtttt atatgcaaac ccaagaggat attaagttct
    13201 ggcctacaac aatggatacc gtaaatttaa ttttatttag tgcattgtgt acagtagtgc
    13261 cttttacctt gtcactttat tgcatgcgtc atattagtgt atttaccgca tcaattaccc
    13321 ttaatcttga gccggtttac ggtattatta tggccattgt gttttttaat gaaaacgaat
    13381 atctggcccc cggattctat atcggaacct tgctcatact actcgtggta tttattaatc
    13441 cgtttattca gcggcgcttt ggtgggccaa ttaaaccgcc agttaccatt ggagaataac
    13501 accgtttgat gtttttatag tacatttacc aaactgttat tgctttttat tgttacttaa
    13561 ccaaggttaa tgtaattcat tatttaagag gagaaaacac gtgagtaaac aatctgctga
    13621 ttttgagttg tttttcagga tttcgattga tgccatgatc attgctgatt tggatggtaa
    13681 aatactcgat tttaatcatg cgtacgaaat aattaccggt tatacccgcg acgagttatt
    13741 tgaactcaac ggagggtggg gacttatttt agatgaagat attcctgcgc tgcgacaaaa
    13801 aatagatgaa ctaaaaacag gggcaagtgt aatgaaaaat tacgaagtgc gccttgttag
    13861 taagagtgga gaaattaaaa ctatttctta cgacgcgaaa gtagatttta ttaatgaaca
    13921 gatttatgcc ataggtcgtg atattactat aataaaagaa caggaaaaat cgctaaaatt
    13981 aaatgaatct cgcttagctt tctttttcga taatgcattt gacggaatgg tgattatttt
    14041 agatggtaaa tatcatttgg taaataaagc atttttacaa atcttgggtc tgcaacatgc
    14101 tgacgatgta ataggtaaaa gttatttaga ttttgtacat gaatcatcaa aagaaaaggc
    14161 gcaaagtata atttctgacg attcagaagc actgtatgaa ttagatattg taagagcaga
    14221 tggcagtttg cgcaatattg aaattgcagg taagtcgcta atatttaata accagagaat
    14281 gcgcattgcc gtaattcgtg atcttgacga ccgccgaaat atgttgcaaa aggcgcagga
    14341 aaatgaggat aggttccgag caatttttca aaattcaaca atgggtattg tgcttactga
    14401 atttgatggt acaatattgg aaataaatca aacattagcc gaacaattag gatatagtgt
    14461 tgctgaatta atgggtggca gtgtattgga tttaatttat gaacccgata aacctgcagc
    14521 tttaaaaggc gctaccatgt tatatgaagg aaaagtggat atttcatatg cagagcggcg
    14581 tctaattaaa aaaactaatt caataacatg gaccaggatg acctgcagtg taattacaac
    14641 tacaggcaat aggaaaatgg tattggggat cctcgaaaac atcgacaatc agaaacataa
    14701 cgaaaaagca cttattgaaa gtgaagagaa atttcgtgca gtttatgaaa gctcaccaat
    14761 gggtattctc atcacaaaaa acccgggtgt tatagttgat gttaatcctg catttgctga
    14821 aatgataggt tttcaatttt ataataggaa cattattgca aacacgaata aatacaattt
    14881 caaaatggaa gaatactttc atttgcatga gaaaattctt ttttcaacga tcgctcagcg
    14941 caaaaagatc aaaggtttgg taaaaatgcg tgtcgatatg atcgttctgg ccacaatttg
    15001 tacagctgtt gtattgcgtg aaacaggaat caagaaaatg tctttatcga agtatgccct
    15061 taaagaagga gcattaagtg cagttattgc caat
//
LOCUS       DAORKW010000026        14875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_46_length_14875_cov_5.835155, whole genome shotgun sequence.
ACCESSION   DAORKW010000026 DAORKW010000000
VERSION     DAORKW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14875
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46_length_14875_cov_5.835155"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..729
                     /locus_tag="PLB46_02245"
     CDS             79..729
                     /locus_tag="PLB46_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75362.1"
                     /translation="MSDVSKIRIKAEYSGLTYETNGLDNVLMSCDLLLPVELLEFKAE
                     VAGIGQSKLTWTTASENNCSGFQVEKSINFTEFDSIGFVPGNGTTTNIHTYDFYDNNF
                     TTSAYYRIKEVNEEGRMFSSDIVYLKNENSNIVTTMVYPNPTSDNIMLVADDVNPVLQ
                     YIITDYEGRTLLGAPVDPYTGLFKKSLSMAQFPSGIYTVTFVKAQGIDAQLFTLIK"
     gene            847..1395
                     /locus_tag="PLB46_02250"
     CDS             847..1395
                     /locus_tag="PLB46_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF75363.1"
                     /translation="MNNASNIQANEEALIIGLQAQDSSVLKIIYEKYASALYGVITRL
                     VNDDHLAEDVLQEAFLKIWNNAASYEAEKGRLFTWMMNICRNAAIDKLRSKGVKNSKQ
                     TSSDETVLLENPTKIYDDIKPDTIGLKELVDKLKPEWKQLVDLIYFKGYTHQEVAEEL
                     NMPLGTVKTRLRSAITELRNII"
     gene            1410..2207
                     /locus_tag="PLB46_02255"
     CDS             1410..2207
                     /locus_tag="PLB46_02255"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HRF75364.1"
                     /translation="MNINEYISSGILELYVTGDLSQAERLEVEALAAKHPEIRKELTA
                     IEEAMEKYAMLHAVEPPASTLNTILTKIEQTQQQEVKGKIVQMASQPKNNTLRLLAFA
                     ASILLFLSVATNIYYFNKYKTVSSELADIREDNTFMAGEMETLKANYQNLESEVNIVK
                     NPDFIAITMKGLPISPASTSVVYWDKANGDLYVNANNLPAPGLGKQYQLWALKDGKPI
                     DAGVFDINGNIQQMKHISEADAFAVTLEPAGGSVAPTLELLYVYAGV"
     gene            2342..2722
                     /locus_tag="PLB46_02260"
     CDS             2342..2722
                     /locus_tag="PLB46_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRF75365.1"
                     /translation="METEVVNSQLIDYAAIFVQFVVAAGFVLTTIYFAQKIGPKHKSV
                     KKFESFECGLDSIGNARLPFSVKYFLVAILFVLFDVEVIFFYPWAVNFRELGESGFVK
                     MLAFIAPLLLGYFYIIKKGALKWE"
     gene            2809..3345
                     /locus_tag="PLB46_02265"
     CDS             2809..3345
                     /locus_tag="PLB46_02265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HRF75366.1"
                     /translation="MVKVTEAPEMYTGEGYGLTRLEKIVGLARANSIWPLPFATSCCG
                     IEFMATMGATYDLGRFGAERLSFSPRQADLLMVMGTISKKMGPVLKQVYIQMAEPKWV
                     IAVGACASSGGIFDTYSVLQGIDKVIPVDVYVPGCPPPPEAILDGIIKIQELIKTESF
                     RRRESEEYQKLLTSYGIE"
     gene            3342..3851
                     /locus_tag="PLB46_02270"
     CDS             3342..3851
                     /locus_tag="PLB46_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRF75367.1"
                     /translation="MSDKVENGLIESLLKEQFGDAIRSVEESYGMLDIVVKREKIIEI
                     ITWLRDSSLLSFNFLTSLCGMHFPDNKGEELGVVYHMHSFANNKRIRLHILFSISDPV
                     VPTVTGLYRTANWMERETFDFFGIKFTGHPDLRRILNMEDMQYHPLLKQYPLEDGTRT
                     DKEDKYFGR"
     gene            3901..5145
                     /locus_tag="PLB46_02275"
     CDS             3901..5145
                     /locus_tag="PLB46_02275"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRF75368.1"
                     /translation="MSDIQLSNNKKDRQFLNGVEQSDEELKQYNYLNLGPTHPATHGI
                     FQNILKMDGEIIIDAQPTIGYIHRAFEKLAEHRPYYQVTPITDRLNYCSSPINNIGWH
                     MTVEKLLNVKTPKKVDYMRVIIMELARIADHIVCNSVIAVDTGALTGFLYVFQWREKI
                     YELYEQICGARLTTNIGRIGGFERDFTPDVYRRLDEFLKDFPKALKEFENMVMRNRIF
                     MDRTIKTGPISGERALEYGFTGPNLRAAGVDYDVRVMTPYSSYEDFDFIIPIGTQGDT
                     YDRFCVREEEMWQSLSIIRQALDKMPKTNEFFADVPEFYLPPKDAVYNTMEGLIWHFK
                     IVMGEVNVPKGQVYHCVEGGNGELGFYLVSDGGRQPFRLHFRRPCFIYYQAYPEMIKG
                     ALLSDAIITMSSMNVIAGELDA"
     gene            5186..5707
                     /gene="nuoE"
                     /locus_tag="PLB46_02280"
     CDS             5186..5707
                     /gene="nuoE"
                     /locus_tag="PLB46_02280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRF75369.1"
                     /translation="MTEVQKIEFSPEAMLEVSRIVSHYPQDKMKSALLPVLHIAQNEF
                     GGWLSAETMDYVARLLHIHPIEVYEVATFYSMYNTKPVGKILIEVCQTGPCCLNGAEK
                     IVSYIEKKLNIKVGETTKDNMFTLKTVECLGACGYAPMFQIGETFYEHLTEEKVDQIL
                     ENLKQEKLKAINN"
     gene            5735..7081
                     /gene="nuoF"
                     /locus_tag="PLB46_02285"
     CDS             5735..7081
                     /gene="nuoF"
                     /locus_tag="PLB46_02285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRF75370.1"
                     /translation="MGRKLLLEHLNKANINTMEGYLQNGGYRSVEKALKKMTPDEITE
                     EVKKSGLRGRGGAGFATGMKWSFLDKNSGKPRYLLCNADESEPGTFKDRLLMEKIPHL
                     LIEGMIVSSYALGCRTSYIYIRGEYMFILRILEKAIAEAYAKGFLGENILGSGYSLDL
                     HVHPGAGAYICGEETALIESLEGKRGNPRIKPPFPAIAGLWGCPTIVNNVESIAAVVP
                     IINDGGDEYAKIGIGKSTGTKLISASGNINKPGVYEIEMGISVEEFIYSDEYCGGIKN
                     NLELKACVPGGSSVPILPKHLLLKMSDGNPRLMTYESLSEGGFATGSMLGSGGFIVYD
                     EKACIVRNTWNFARFYHHESCGQCSPCREGTGWMERVLHNIEYGHGKMEDIDLLWDIQ
                     RKIEGNTICPLGDAAAWPVAAAIRHFRHEFEYHVKYPNRVFDEDHVYQPEMKMQKV"
     gene            7109..8056
                     /locus_tag="PLB46_02290"
     CDS             7109..8056
                     /locus_tag="PLB46_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRF75371.1"
                     /translation="MPKVTIDDITIDVPAGTTILEAARMIGENVPPAMCYYSKLKGSG
                     GKCRVCLVKVTKGSEKDPRPMPKLVASCRTAVMDGMEVKNVTSPEVIEARKGVVEFLL
                     VNHPLDCPVCDQAGECHLQDLSYEHGAAQSRYEFKRRTFETRDIGDKIKLHMNRCILC
                     YRCTQVADQLTDQRVHGVLGRGEVSEISTYIETAIDNEFSGNMIDVCPVGALTDKTFR
                     FKSRVWFTKPVYAHRNCTKCTGKVTLWYQGEEVLRVTSRKDQWGESEAFICNECRFDK
                     KATADWKIEGPAHVDRHSVISQNHYELPFELPVTQKKLQ"
     gene            8330..9370
                     /gene="nuoH"
                     /locus_tag="PLB46_02295"
     CDS             8330..9370
                     /gene="nuoH"
                     /locus_tag="PLB46_02295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRF75372.1"
                     /translation="MIDPILIFKIILVVVLFALSLAIAAYSTYAERKVAAFMQDRLGP
                     NRAGPFGLLQPLADGVKMFMKEDFIPAASNRFLFIAGPAITMITALMTSAVIPWGPNV
                     TINGTSVPLQVADINIGVLYVLGVVSLGVYGIMIGGWASNNKYSLLGAVRASSQMISY
                     ELAMGMSIIAVVMSTGSLSLGSIVNAQSDWAGMNWNVFVQPLGCLIFIICAFAECNRT
                     PFDLPESETELVGGFHTEYSSMKLGFFLFAEYVNMFISSAMITCLYFGGYNFPGMEAV
                     QGSVGEIWFVLICVAVMFAKVLFFVFFFMWIRWTIPRFRFDQLLNLGWKVLLPLSILN
                     VLITGAVIIFLN"
     gene            9567..9956
                     /locus_tag="PLB46_02300"
     CDS             9567..9956
                     /locus_tag="PLB46_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRF75373.1"
                     /translation="MHPDLLKARTKKFAIVCLEICKRIPPVPENNVLKIQLSKSGTSV
                     GANYRAACRARSQNEFIAKLSIVNEEADESCYWLEVITEGKVLADAETIKLLKEANEL
                     NAIFTASAKTAKMNRWLKQQGDKKIDS"
     gene            10048..10596
                     /gene="nuoI"
                     /locus_tag="PLB46_02305"
     CDS             10048..10596
                     /gene="nuoI"
                     /locus_tag="PLB46_02305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRF75374.1"
                     /translation="MQSLTNRSRVVTNKKMTLSERLYLPAILGGLRITMSHFFTKKVT
                     VNYPEETREHSKVFRGVHVLKRDEEGRENCTACGLCAVACPAEAITIVADERQKGEEH
                     LYREEKYAAEYNINMLRCIFCGLCEEACPKDAIYLTDRIAPSNYQRDQFLFTKDMLVE
                     PIEKDKRIDISSRRKGIREGAN"
     gene            10631..11134
                     /locus_tag="PLB46_02310"
     CDS             10631..11134
                     /locus_tag="PLB46_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRF75375.1"
                     /translation="MSEIFFYALSAIAIACSLLMLFQRNAMYSVLYLLVCFFAVAGLY
                     IMLNAQFLAVVHIIVYAGAIMVLFLYVLMFLNLNTPAAFTKTNFLRMAAIMTGGSLFL
                     LLFASFYKSELVEMPMPVTNTTGDVQTLGKVLFNEFLIPFELTSVLFLSAMVGVIIMN
                     KKDKANG"
     gene            11127..11465
                     /gene="nuoK"
                     /locus_tag="PLB46_02315"
     CDS             11127..11465
                     /gene="nuoK"
                     /locus_tag="PLB46_02315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRF75376.1"
                     /translation="MDNTGALSEQMRAIPLENFIMLSLILFSIGVMGILMRRNLIVVF
                     LSIELMLNSINLMAAAFSSYHNDPQGQAFVFFIMVVAAAEVAVGLAMLVMIYRNTKSI
                     DINSLDKLKW"
     gene            11537..13432
                     /gene="nuoL"
                     /locus_tag="PLB46_02320"
     CDS             11537..13432
                     /gene="nuoL"
                     /locus_tag="PLB46_02320"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRF75377.1"
                     /translation="MSQNTLNIITLLIPALPLLGFFIHVFFKNKISEKLAGIHASLWV
                     ALSFVLSLVLFNQLQSVEVIHVQLCNWFSVGGLTIPFAFQIDHLSIIMMMVITGVGSL
                     IHIYSIGYMHGDEGFNRFFSYLNLFIFFMLTLVMANNYILMFVGWEGVGLCSYLLIGF
                     WYKNQEYNKAANKAFIMNRIGDLAMLFGLFLLFQYAGTLTFDGVNESVSRIVPDENLL
                     TAITVLLFIGACGKSAQLPLYTWLPDAMAGPTPVSALIHAATMVTAGIYMIVRSNLLF
                     SLAPFTLEIIMVVGIVTAIFAGSIALKQNDIKKVLAYSTVSQLGLMFFAIGVGAYEAA
                     MFHLITHAFFKALLFLSAGSVIHGLGGEQDIRKMGGLDKRMKITHWVFGFGVLAIMAV
                     PPFSGFFSKDSILIAAYHISPVLWVIGLFAGLLTAYYMMRVYAVVFRGDERMSAEVSK
                     HIHESPRVITTPLIVLTVLAIFGGVINMPHLFGGNKMMMHYLSPVVPMDEKHMAMNTE
                     IILMLIASVAIFLTIYFTHLKYKIKKTVPEADAQITGFGKVLNHKYYIDELYNAIVVK
                     PLNMLGNFLYKIIDLQVIDGFVNGTARFSAYCSSQLKNIQTGAVSFYLFAMALGMVGI
                     LLLIILM"
     gene            13436..>13860
                     /locus_tag="PLB46_02325"
     CDS             13436..>13860
                     /locus_tag="PLB46_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75378.1"
                     /translation="MFTLLLILLPIVAGLAMLMLPQQQAKQIALIAALATLGVTVATY
                     FQFDPKAGTQFVTDVPWINMLGVRFHIGIDGISMVMLILSNILAPLIILSSFKDNYNN
                     ARLFYGLMLVMQGAMNGVFVSMDMFLYYIFWELALIPAY"
     assembly_gap    13861..13870
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13871..>14875
                     /locus_tag="PLB46_02330"
     CDS             <13871..>14875
                     /locus_tag="PLB46_02330"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRF75379.1"
                     /translation="VLWWGKGETSKITFKFFIYTLFGSLFMLVAIIWLSLQGATNSTD
                     ISSVYALQIPAETQLYIFLAFMLAYAIKIPVFPFHTWQPDTYTFSPSPATMLLSGVML
                     KMGLYSVIRWILPVVPDAVNEYGKIIMVLAAVGVFYASAIAWVQKDLKRLFAYSSIAH
                     VGLITAGILTVSEMGLQGSMVQMMAHGINVVGLFYVCQILYRKFNSHDINAFGGLRLN
                     APLFAGLYLVIMLASVALPLTNAFPGEFMLLNSVYAVNPWICLFAGSGVILGAVYMFT
                     SYQKVMLGELNNKTNSFTEIDSLDKWVLIPIVILIFVFGLYPSFITNISEDAVKGIIQ
                     "
BASE COUNT         4665 a         2477 c         3086 g         4637 t
ORIGIN      
        1 gccgcgcccc cgcggcgccc gccgcccgcc gcaggggggg gatcgcccgc cgcaaccgca
       61 gcacaaattt tagctatttt gagtgatgta agtaaaatcc gcattaaagc agaatactca
      121 ggattaacgt acgaaactaa tggacttgac aatgttttaa tgtcctgtga tttactttta
      181 cctgttgaac ttcttgaatt taaagcagaa gttgccggca ttggtcagtc gaaacttacc
      241 tggaccaccg caagcgaaaa caattgcagc ggatttcaag ttgaaaaaag tataaatttc
      301 acagaatttg actctatcgg atttgttccg ggtaacggca caactaccaa tatccatact
      361 tatgattttt acgataacaa tttcactaca tcagcctatt ataggataaa agaagtaaat
      421 gaagagggaa ggatgtttag ttcggatatc gtttatctca aaaacgaaaa cagcaatatc
      481 gttacaacta tggtgtatcc caaccctact tccgataata ttatgttggt tgctgatgat
      541 gttaaccctg ttttacaata catcataaca gattacgaag gaagaacatt attgggagct
      601 cctgttgacc cctataccgg attattcaaa aaaagcttaa gtatggcgca atttccttcc
      661 ggcatatata ctgttacttt tgtaaaggca cagggcattg atgcacagct ttttacactt
      721 attaaataat tattgtcccc aattagccga ttttgatgta atttgatggt tttttaagaa
      781 atttctaaac ttttaaaacc gaacgaacaa tttcctcgta aatttgtcca gtcattaaaa
      841 ccataattga ataacgcttc gaacatacag gcaaacgaag aagcactcat aataggttta
      901 caagcacagg attcttctgt acttaaaatc atttatgaga aatatgcttc cgcactatac
      961 ggtgttatca ctcgtttggt taatgacgac catcttgcag aggatgtttt gcaggaggcg
     1021 ttcttgaaaa tttggaataa tgcggcttct tatgaagcgg aaaaaggcag attgtttacc
     1081 tggatgatga atatttgcag aaacgcagca atagataaac tgcgcagcaa aggtgttaaa
     1141 aacagtaaac aaacatcaag cgacgaaacg gtgctgttgg aaaatcctac caaaatttat
     1201 gatgatatca aaccagatac catcggttta aaagaactgg tagataaact caagcccgaa
     1261 tggaagcagt tggttgacct tatttatttt aaagggtaca cccatcagga agttgctgaa
     1321 gagctgaata tgccattagg gacggtcaaa acaagattaa gaagtgcaat aacagaatta
     1381 cgaaatataa tttaataaga gcaaaagctt tgaatatcaa cgaatacata tcatcaggaa
     1441 tactggaact ttacgttaca ggcgacctaa gtcaggctga aaggcttgaa gtagaagcgc
     1501 ttgcagcgaa acatccggaa attcgcaaag agctcactgc tattgaagag gcgatggaga
     1561 aatatgctat gctgcatgcc gttgaacctc cagcttcaac actcaatacc attttaacca
     1621 aaattgaaca aacgcaacag caagaggtta aaggcaaaat tgtacaaatg gcgtcgcaac
     1681 ctaaaaataa taccctccga ttattagctt tcgcagccag tatcttattg tttttatcgg
     1741 ttgccactaa tatttattac ttcaataaat acaaaaccgt aagcagcgaa ttagctgata
     1801 ttcgcgaaga caatactttt atggcaggag aaatggaaac gcttaaggcg aattaccaaa
     1861 atctcgaatc agaagtcaat attgttaaaa atcctgattt tatcgctatc acaatgaaag
     1921 ggttacctat aagtccggct tccacctcag tagtgtattg ggataaagcc aatggcgact
     1981 tgtatgttaa cgccaacaat cttcctgcac cgggcctggg aaaacaatat caattatggg
     2041 ccttaaaaga cggcaaacct attgatgcag gtgttttcga tatcaacggt aatattcagc
     2101 aaatgaaaca tatatctgaa gctgatgctt ttgcagtaac gcttgaacct gccggtggaa
     2161 gtgtagcgcc aacgctcgaa ttgctttatg tttatgccgg cgtttaacca ttttatttat
     2221 tcgcttattt tactttagct gcacatcaca aattttcgga atttgtagta cgaaaagcat
     2281 gttttttccc atttcatttt cctacctttg ccgtgcgttt taataaatcg cttcgataaa
     2341 aatggaaaca gaggtagtta acagccaatt aattgattac gcagccatat ttgtgcagtt
     2401 tgttgtagct gccggcttcg tacttaccac catttatttc gctcaaaaaa tcggccctaa
     2461 acataagtcg gttaaaaagt tcgaaagttt tgagtgtggt ctcgattcaa ttggtaatgc
     2521 ccgccttcca ttttcagtaa aatatttcct tgtggctatt ctttttgtgt tgtttgatgt
     2581 ggaagtaatt ttcttttacc cttgggcggt taatttccgt gaactagggg aatcaggttt
     2641 tgttaagatg ttagcgttta ttgcgccttt actcctcggc tacttttata ttattaaaaa
     2701 aggtgccctg aagtgggaat aactaactga gactgaacat atttcagccc agccagtctc
     2761 gaaaatttaa ccggttcgcc aaaaacgaat cctaaaaata ttaccgacgt ggtaaaagtt
     2821 acagaagcac cggaaatgta tacaggcgaa ggctacggcc ttacccgcct cgaaaaaatt
     2881 gttggactgg cccgcgctaa ctccatttgg cctcttccat ttgctacctc ttgttgcggg
     2941 attgaattta tggctaccat gggtgccacc tacgatcttg gccgttttgg tgccgaaaga
     3001 ttgagttttt ctccaagaca agctgatttg cttatggtaa tgggtaccat ttctaaaaaa
     3061 atgggacccg tactcaaaca ggtatatatc caaatggccg aacctaaatg ggtaattgcc
     3121 gttggtgcct gcgcatcaag tggcggtatt ttcgatacct attcagtgtt acagggtatc
     3181 gataaagtaa ttcctgttga tgtatacgtg cctggttgcc ctcctccgcc ggaagcaatt
     3241 ttagatggca ttattaaaat tcaggaactg attaaaaccg aaagcttcag aagaagagaa
     3301 tcggaagaat atcaaaaatt attgaccagc tacggaatag aatgagcgat aaagtagaaa
     3361 acggattaat cgaaagcctc ttaaaagagc aattcggcga tgccatacgc agcgtggaag
     3421 aatcatacgg tatgcttgat atcgtagtga aacgcgaaaa aattatcgaa attattacct
     3481 ggttgcgcga tagtagtctg ctaagtttta attttttaac cagcctctgc ggaatgcatt
     3541 ttcccgataa taaaggagaa gaattaggtg tggtttacca tatgcacagt tttgccaata
     3601 ataaacgcat tcgtttacat atcctgtttt caatcagcga ccctgttgtg ccaactgtaa
     3661 ccggattata tagaactgca aactggatgg aacgcgaaac attcgatttt tttggcatta
     3721 aattcaccgg ccaccctgat ttaagaagaa tattgaatat ggaagatatg cagtatcatc
     3781 cgttgttaaa acaatatccg cttgaagacg gaaccagaac cgataaagaa gataaatatt
     3841 tcggacgtta accgaactta ataaaagggc gaaattattt ttccccaaat aaaaaaaacg
     3901 atgagtgata ttcaattaag caataacaaa aaagaccgcc agtttttaaa tggtgtggag
     3961 caatctgatg aggaattaaa acaatataat tacctcaacc tcggaccaac tcacccggct
     4021 acgcacggta ttttccaaaa cattttaaaa atggatggag aaataattat cgatgctcag
     4081 cctactatcg gttatattca tcgcgctttc gaaaaactgg ctgaacatcg cccgtattat
     4141 caggtaacac caattaccga ccgcttaaat tattgttctt ctccaattaa taatatcggc
     4201 tggcatatga cagtcgaaaa attactcaat gtaaaaacac ctaaaaaagt ggattacatg
     4261 cgggtaatta ttatggaatt agcacgtatt gccgaccata ttgtttgtaa ttcggtaatt
     4321 gccgttgata ccggagcatt aaccggattt ttatatgtat ttcagtggag agaaaaaatt
     4381 tacgaattat acgaacaaat ttgcggcgca agattaacta caaatattgg tcgtattggt
     4441 ggatttgaac gcgattttac acctgatgtg tatcgccgtt tagatgagtt tctaaaagat
     4501 ttccctaaag cattaaaaga attcgaaaac atggtaatgc gcaaccgcat tttcatggat
     4561 agaacaatta aaacaggacc tatttccggc gaacgcgctt tggaatacgg atttactgga
     4621 ccaaatttac gcgctgcagg tgtagattat gatgtgcgtg tgatgacacc ttacagcagt
     4681 tatgaagatt ttgattttat tattccgatt ggaactcagg gcgatactta cgatagattt
     4741 tgtgttcgtg aagaagaaat gtggcaaagc ttaagtatta ttcgtcaggc tttagataaa
     4801 atgcctaaaa caaatgaatt ttttgccgac gttcctgaat tttatttgcc tccgaaagat
     4861 gcagtttata ataccatgga aggattaatc tggcatttta aaattgtaat gggcgaagta
     4921 aatgttccaa aaggtcaggt atatcattgt gtggaaggtg gaaatggtga gttaggattt
     4981 tatttagtga gcgatggtgg tcgtcagcct ttccgtttgc atttcagaag accatgtttt
     5041 atttattatc aggcatatcc tgaaatgatt aaaggtgctt tattaagtga tgccattatt
     5101 accatgagca gcatgaacgt aattgcagga gaattagatg cgtaatttat tgatctcctg
     5161 aaagggagta atttttatat tagtaatgac agaagtacaa aaaattgaat tttcaccgga
     5221 agcgatgctc gaagttagcc gcatcgtaag tcattatccg caagataaaa tgaaatctgc
     5281 gctgttgccg gtgttacata ttgcacaaaa cgaatttggc ggatggttaa gtgcagaaac
     5341 aatggattat gttgcgcgat tgttacatat tcatccaatt gaagtgtatg aagtggcaac
     5401 attttattcg atgtataata ccaaaccggt aggaaaaatt ttaattgaag tgtgccagac
     5461 aggtccgtgt tgtttaaatg gtgctgaaaa aattgtcagc tacatcgaaa aaaaattaaa
     5521 tatcaaagtt ggtgaaacaa caaaagataa catgtttact ttaaaaactg tggaatgttt
     5581 aggtgcttgt ggatatgcac ctatgtttca aattggcgaa acattttatg agcatttaac
     5641 ggaagaaaaa gtagatcaga ttttagaaaa tctgaaacag gaaaaattaa aagcaataaa
     5701 taattgatgc tcacgtagca tatcaaatca tactatgggt cgcaaattat tattagaaca
     5761 tctgaataaa gccaacatca acacgatgga aggttacctt caaaacggag gttatcgttc
     5821 agttgaaaaa gctttaaaaa aaatgacacc tgatgaaatt accgaagagg taaaaaaatc
     5881 aggattgcgc ggtcgcggcg gcgctgggtt tgctacggga atgaaatgga gtttcctcga
     5941 taaaaattca ggtaaaccac gttatttatt atgtaatgca gatgaaagtg aaccgggcac
     6001 atttaaggat agattattaa tggaaaaaat ccctcaccta ttaattgaag ggatgattgt
     6061 ttccagttat gctttaggtt gtcgtacttc ctatatttat atccgcggtg aatacatgtt
     6121 tattttgcgc attttagaaa aggcaattgc tgaagcttac gctaaaggat ttttaggaga
     6181 aaatatttta ggtagcggat attcactcga cttacatgtg catccgggtg caggtgctta
     6241 tatctgtggt gaagaaacgg cattaatcga atcacttgaa ggtaaacgtg gtaacccgcg
     6301 aattaaacca ccatttcctg ctattgccgg attatggggt tgccctacaa ttgtgaacaa
     6361 tgttgaatct attgctgctg ttgttcctat tattaacgat ggtggcgatg aatatgcaaa
     6421 aatcggtatt ggaaaatcaa ccggtacaaa attaatttcg gcatcaggta atattaataa
     6481 acctggagtg tatgaaattg aaatgggaat ttcagttgaa gaatttattt attcagatga
     6541 atattgcgga ggcataaaaa ataatttgga attaaaagcc tgtgttccgg gtggaagttc
     6601 agtgccaatt ttgccaaaac atttattgtt aaaaatgagt gatggtaatc cgcgtttaat
     6661 gacttacgaa agtttaagtg aaggtggatt tgcaaccgga agtatgttag gctccggcgg
     6721 atttattgtg tatgatgaaa aagcatgtat tgttcgcaac acatggaact ttgcacgttt
     6781 ttatcatcat gaaagttgtg gtcagtgttc accttgccgc gaaggaaccg gctggatgga
     6841 acgtgtgtta cataatatag aatacggtca cggaaaaatg gaagatatag atttgttatg
     6901 ggatattcag cgcaaaattg agggcaatac catttgtcct ttaggtgatg cagctgcatg
     6961 gcccgtagct gcagcaattc gtcatttccg tcacgaattt gaatatcatg tgaaatatcc
     7021 gaatcgcgtg tttgatgaag accacgtgta tcaaccggaa atgaaaatgc aaaaagtgta
     7081 aagcaattaa ttgttataaa ataataacat gccaaaagta acaatagacg atatcaccat
     7141 tgacgttccc gcaggaacta ccattctgga agcagccaga atgataggcg aaaatgtgcc
     7201 gccggcaatg tgttattatt ccaaattaaa aggaagcgga ggaaaatgtc gcgtgtgttt
     7261 ggttaaagta accaaaggaa gcgaaaaaga cccgcgccct atgccaaaat tagttgcgag
     7321 ttgtcgcact gcggtgatgg atggcatgga agtaaaaaat gtaacctctc ctgaagtaat
     7381 tgaagcaaga aaaggtgtgg tggaattttt attggtaaac catcctctcg attgccccgt
     7441 ttgcgatcag gccggtgaat gtcatttaca ggatttaagt tacgagcacg gtgctgcaca
     7501 aagccgttac gaatttaaaa gaagaacttt tgaaacaaga gatatcggcg ataaaataaa
     7561 attgcatatg aatagatgca ttttatgtta tcgttgtaca caagttgccg accagttaac
     7621 tgatcaacgc gtgcatggtg ttttaggaag aggtgaagtt tctgaaattt caacttatat
     7681 cgaaacagcc atagacaacg aattttcagg taatatgatt gacgtttgtc cggttggtgc
     7741 tttaacagat aaaacattcc gttttaaatc aagagtgtgg ttcaccaaac ctgtgtatgc
     7801 gcatcgtaat tgcacaaaat gtacaggtaa agttacactg tggtatcagg gagaagaagt
     7861 gttgcgtgta accagccgca aagatcagtg gggcgaaagt gaagcattta tctgtaacga
     7921 atgtcgtttc gataaaaaag caactgccga ttggaaaatt gaagggccgg cacatgttga
     7981 ccgccactcg gtgatttcac aaaatcatta cgagctgcct ttcgaattac cggtaacgca
     8041 gaagaaattg cagtagggaa aaaaatgaac agccgattga gcaggattga aacaatcccg
     8101 atagttatca ggagagcaaa attgaatcaa gtttgatagg gattagggga atcaaaatag
     8161 agttattccg aaatcaatta ggttaaactt aattgatttt tataaataaa ttttataaat
     8221 aaaaagaaca agtagaagca gtttttaacc ataaagagcg aaccgaaatt ttgttcaatt
     8281 ttgttcaatt ctgtttcaat aataaaatac caaacaccgt atcatccaaa tgatcgaccc
     8341 gatattaata tttaaaataa ttctcgttgt agtgcttttt gcattatcac ttgcaattgc
     8401 cgcatatagt acttatgctg aacgtaaagt agcagcattt atgcaagacc gccttggacc
     8461 aaatcgtgca ggaccatttg ggttgttgca acctttagcc gatggtgtta aaatgtttat
     8521 gaaagaagat tttattcctg cagcatctaa tcgattttta tttattgcag gtcctgctat
     8581 tactatgatt acggcattaa tgacaagtgc ggtaattcca tggggaccaa atgttaccat
     8641 taatggaacc agtgtaccat tgcaggtagc ggatattaat attggtgtat tatatgtatt
     8701 aggagttgtg agtttagggg tttacggaat tatgattggt ggatgggctt ctaataataa
     8761 atattcatta cttggagccg ttcgtgcatc aagccaaatg attagttatg aattagcaat
     8821 gggtatgagc ataattgctg ttgtaatgtc aaccggctca ttaagtttag gaagtattgt
     8881 aaatgcacaa agcgattggg caggtatgaa ttggaacgta tttgttcagc cactcggatg
     8941 tttaattttt attatttgtg cttttgctga atgtaacaga acacctttcg atttgcccga
     9001 atcggaaacc gagttagttg gtggattcca tactgaatac agttcaatga aacttggatt
     9061 ctttttattc gccgaatatg taaatatgtt tatctcaagc gcaatgatta cctgcttgta
     9121 ttttggcgga tataattttc caggtatgga agcggttcag gggtcagttg gtgaaatatg
     9181 gtttgtgctg atttgtgtag cggtaatgtt tgctaaagtt ttgttcttcg tattcttctt
     9241 tatgtggatt cgctggacaa taccaagatt ccgtttcgac cagttgttga atttaggatg
     9301 gaaagtgtta ctcccgttgt cgatattaaa tgtattgatt acaggagcgg tgattatatt
     9361 tttgaattga agcaaaattg aacaaaattg aacaaaattg agcaagattg aagcaaaatt
     9421 gagcaatccc gatagctatc gggagaagca aaattgaaca agttagaaat catctcgata
     9481 tttatcgaga ataaaattta aaaattttgc ctcaattttg atcaattaat aaatatttta
     9541 ttttttaacc cttagaaata gtttttgtgc atcctgattt attaaaggcc aggacgaaaa
     9601 aatttgcaat agtttgttta gaaatttgca aacgtattcc tccggtgcct gaaaacaatg
     9661 ttcttaaaat acaattatca aaatccggca cctctgtagg agctaactat agggcagctt
     9721 gtagagcaag gtctcaaaat gagtttattg ctaagttgtc aatagttaat gaagaagcag
     9781 atgaaagttg ttattggttg gaagtgatta cagaaggcaa agtattagcc gatgctgaaa
     9841 ctattaaatt gcttaaagaa gcaaatgaat taaatgccat ttttacggca tcggcaaaaa
     9901 cagcgaaaat gaatcgttgg ctcaagcaac aaggtgataa gaagattgat agttgagaaa
     9961 aaattgaaca aaattgaaca aaattgaaca agattgatca aaattgaaca atcttgttca
    10021 atcttgatca attaaaaaat acaaacaatg caaagtttaa ctaacagaag tagagtagta
    10081 acaaacaaga agatgaccct ctcggagcgt ctttacttgc ctgctatttt gggtggattg
    10141 cgcattacga tgtcgcattt tttcactaaa aaagttaccg taaactaccc tgaagaaacg
    10201 cgcgaacaca gcaaagtgtt tcgcggagtg cacgtgttga aacgcgatga agaaggacgt
    10261 gaaaattgca ccgcttgcgg actttgtgct gttgcctgcc ctgcagaagc aattacgata
    10321 gttgccgatg aacgtcagaa aggtgaagaa catttatacc gtgaagaaaa gtatgctgca
    10381 gaatataata ttaatatgtt gcgttgcatt ttttgcggtt tatgcgaaga agcttgtcca
    10441 aaagatgcca tttatttaac cgatagaatt gcaccaagta attatcagcg tgatcaattt
    10501 ttgtttacta aagatatgtt agtagaaccg attgaaaaag ataaacgaat tgatatcagc
    10561 tcacgacgca aaggaattcg cgaaggagca aattaattaa atagtagaac ccaatacaga
    10621 acccgatata atgagtgaaa tattttttta cgcactttca gcaatagcaa tcgcttgtag
    10681 tttgctgatg ttgtttcagc gcaacgctat gtatagtgtg ttatacctgc tcgtatgttt
    10741 ttttgcagta gccggattat acatcatgct gaacgcacaa tttcttgctg ttgttcatat
    10801 tattgtttac gccggcgcca ttatggtgtt gtttttatat gtattgatgt ttctcaacct
    10861 gaacacaccg gcggcattta cgaaaactaa ttttttacga atggctgcca taatgaccgg
    10921 cggttctttg tttttattat tgtttgcttc attttataaa tctgaattgg ttgaaatgcc
    10981 aatgccggtt accaatacaa caggtgatgt gcaaacatta ggaaaagtat tatttaacga
    11041 atttcttatt ccgtttgagc tgacaagtgt tttattcctg tcggcaatgg tcggagtaat
    11101 tattatgaat aaaaaagata aagcaaatgg ataatacagg cgctttgagc gaacaaatga
    11161 gagctatccc gctcgaaaat tttatcatgc tttcgctgat attattcagc attggcgtga
    11221 tgggaatttt aatgcgtcgc aatttaattg tggtgttttt aagtattgaa ttaatgttga
    11281 attcaattaa cctgatggca gcagcattct cttcgtatca taatgatcca caagggcagg
    11341 cttttgtgtt ttttattatg gtagtagcgg ctgccgaagt tgcagttggc ttggcaatgt
    11401 tggtgatgat ttacagaaat acgaaatcaa tagatatcaa ttcattagat aaattgaagt
    11461 ggtaatccaa ataataaata attaatcaaa ccatctgctt cacaagcatg atgataaatg
    11521 ataaaaagaa attaaagtga gtcaaaatac cctaaatatc attaccctgc ttattccggc
    11581 attgccattg cttgggtttt ttatacatgt ctttttcaag aataaaattt ctgaaaaact
    11641 ggccggtatc catgcttctt tgtgggtggc actttcgttt gtgctttcac ttgtgttatt
    11701 taatcaactg caatcggttg aagttattca tgtacaatta tgtaattggt tttcggttgg
    11761 tggtttgaca atccctttcg cattccaaat cgaccacctg agtatcatta tgatgatggt
    11821 gattaccgga gttggtagtt taatacatat atactccatc ggatacatgc atggtgatga
    11881 aggatttaat cgtttctttt catatctgaa tcttttcatt ttctttatgc ttaccctggt
    11941 aatggcaaac aactatattc tgatgtttgt tggctgggaa ggtgttggtt tgtgttctta
    12001 tttattaatt ggattctggt ataaaaatca ggaatataat aaagcagcaa acaaggcatt
    12061 tattatgaac agaattggcg acttagccat gttgttcggg ttgtttttat tatttcaata
    12121 tgcgggaaca cttacattcg atggcgtaaa tgaaagtgta tctcgaattg ttccggatga
    12181 aaatttgtta accgcaatca cggtattatt atttattggt gcctgcggaa aaagtgctca
    12241 gctaccactt tatacctggt tgccagatgc aatggccggt ccaacaccgg tttctgcatt
    12301 aatccatgcg gctactatgg tgactgccgg tatctatatg attgtgcgca gcaacttgtt
    12361 gtttagtctg gctccgttta ctttagaaat aattatggtg gtaggaattg tgactgcaat
    12421 ttttgcaggt tctatcgcct taaaacaaaa tgatattaaa aaagtattgg cctactcaac
    12481 agtaagtcaa ttaggtttaa tgttttttgc tataggtgta ggagcatacg aagcagcaat
    12541 gttccactta ataacccatg cattctttaa ggccttatta ttcctttcag caggaagtgt
    12601 tattcacggc ttgggaggtg aacaagatat tcgcaaaatg gggggcttgg ataagcgtat
    12661 gaaaattacg cattgggtat ttggatttgg cgtgttggca attatggctg tgccaccttt
    12721 ttccggattc ttttcaaaag atagtatcct aattgccgcc tatcatattt cacctgtgct
    12781 ttgggtaatt ggtttatttg caggattact tacagcatat tatatgatgc gcgtttatgc
    12841 agtggtattc agaggcgatg aacgtatgtc ggctgaagtg agtaaacata ttcatgaatc
    12901 acctcgtgtt attactacac cattaattgt gctgacagtg ctggcaattt ttggtggtgt
    12961 tattaatatg ccacatttat ttggtggcaa taaaatgatg atgcattatt taagcccggt
    13021 agtgcctatg gatgaaaaac atatggcgat gaatacggaa attatactca tgttaatcgc
    13081 atctgttgcc atatttttaa ccatctattt tacgcactta aaatataaaa ttaaaaagac
    13141 agtacctgaa gccgatgcac aaattaccgg ttttggaaaa gtattaaatc acaaatatta
    13201 tatcgacgaa ttatataacg cgattgttgt aaaaccttta aacatgttag gcaacttcct
    13261 gtataaaatt attgacttac aggttattga cggatttgtg aatggaactg cacgattctc
    13321 ggcatattgt tcatcacagt taaaaaacat acaaaccgga gcagtaagtt tttatttatt
    13381 tgcaatggca cttggtatgg ttggaatttt attattaatt attttgatgt aattcatgtt
    13441 cacattacta ctcatattat tacccattgt tgcaggatta gcgatgctga tgttaccgca
    13501 gcaacaagcg aaacaaattg cactgattgc tgcattggca acacttggtg ttaccgtagc
    13561 cacttatttc cagtttgacc caaaagcagg gactcaattt gttacagatg taccttggat
    13621 taacatgtta ggtgtgcgtt tccacatcgg tatcgatggt atcagtatgg tgatgttgat
    13681 tttgtctaat atcctcgctc cacttattat tttatcttca tttaaagata attacaataa
    13741 tgcacgatta ttttacggtt taatgttagt catgcaaggc gccatgaacg gggtgtttgt
    13801 ttcaatggat atgttcctgt attatatttt ctgggaactt gcattaattc ctgcttattt
    13861 nnnnnnnnnn ggttttatgg tggggaaaag gagaaacttc aaaaattacg tttaagtttt
    13921 ttatttacac cttattcgga agtttattta tgctcgttgc cattatctgg ttgagcttac
    13981 aaggtgcaac taattcaact gatatcagca gtgtgtatgc tttacaaatt cctgctgaaa
    14041 cgcaattata tattttcctt gcatttatgt tggcttatgc catcaagata ccggtgtttc
    14101 catttcatac ctggcaacct gatacctata cattttcgcc atcaccggca accatgttgt
    14161 tatcaggtgt tatgttaaaa atgggtttat attcagtaat tcgctggata ttacctgtgg
    14221 ttcccgatgc agttaatgaa tacggtaaaa taataatggt gcttgcggca gttggtgtat
    14281 tttatgcctc agcaattgcc tgggtgcaaa aagatttaaa acgattattt gcttattcat
    14341 caattgcaca cgtaggttta attactgccg gtattttaac agtgagtgaa atgggcttac
    14401 aaggaagtat ggtgcaaatg atggcacatg gtattaatgt ggtaggtttg ttttatgtat
    14461 gccagatttt atatcgcaaa tttaattcgc acgatataaa tgcctttgga ggattgcgtt
    14521 taaatgctcc tttatttgca ggattatatt tagtaattat gctggcttct gtcgcactgc
    14581 cattaaccaa tgcgtttccg ggtgaattta tgttgcttaa ttcagtttat gccgtaaatc
    14641 catggatatg tctatttgca ggtagcgggg ttattttagg tgcggtttat atgtttacta
    14701 gttaccaaaa agtaatgttg ggcgaactta acaataaaac aaattcattt accgaaatag
    14761 atagtttaga caagtgggtg ctaattccga ttgtaatttt aatttttgtt ttcggattgt
    14821 atccatcgtt tattaccaat atttcggaag atgcggtaaa aggaattatt caaat
//
LOCUS       DAORKW010000027        14664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_49_length_14664_cov_4.560682, whole genome shotgun sequence.
ACCESSION   DAORKW010000027 DAORKW010000000
VERSION     DAORKW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14664
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49_length_14664_cov_4.560682"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..758
                     /locus_tag="PLB46_02335"
     CDS             201..758
                     /locus_tag="PLB46_02335"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF75380.1"
                     /translation="MEISITKSKMGSSKAATSTHSPLSLEDMYKVFAKKMINLSARIV
                     NNPITAQDIVQDAFISGHAHIHQFTTETDFGNWLKRVVVNKSIDQLRKKSNATFEIDE
                     TTHLVAETDEISEEPEYDIPTVLQSIAELPDGYRLVFTLFVFEQYSHNEIAAALNISE
                     GTSKSQYNRARKKLIQIIQSKQIKS"
     gene            755..2179
                     /locus_tag="PLB46_02340"
     CDS             755..2179
                     /locus_tag="PLB46_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HRF75381.1"
                     /translation="MSKLRIEDFILQNKDAFDIIEPAPIAATGILSSTTKIVSSSIKS
                     ITMKVLAIISITTVTVISYLTFSNRENTSAPTTKGGEQTVQASTMNVSEEPPTTNQTS
                     AIYPGHENPITHLEKHSPLTILDEQIEPDPEIINTTYIQQTWDSIQMRQPETYSWVRK
                     GKSVTLNIDTLFTGITHVEINTNSFDVIAQGQTGSHNVNVKVEAENKIKGIVINDPKI
                     EVVCTLEGSVLKIKALFGGSSGIGTFESDGTLTVNVPETATIKINHDYGNLDVSNLIT
                     NLFDVNASSSNVKINNITAPINVRVDYGNVQLDKTVGALNMDLGSCNVTCGNQKGDVK
                     IESDYCNLSMGGIQGNINGKLNSGKVDILDITGNIKFNADYTNIKMNNVKGDLSMVTN
                     SGNIVGEKIELISAANFNTEYGSVDIDFVNDIDKMSFDLKTSYGNIQLQDGVNARKTQ
                     GELLIERGGIKVSSTTDSGNIKIK"
     gene            complement(2332..3402)
                     /locus_tag="PLB46_02345"
     CDS             complement(2332..3402)
                     /locus_tag="PLB46_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRF75382.1"
                     /translation="MKIHSVRAFLERIPLDQPYAIAYQSIVDTEIIYFIVTLDNGIQG
                     FGASNPFPEVIGETPALTLQNLQSDFIQELVGKNIEDYPQIIAQCRQQFPHMPGTIAA
                     IDIALLDAYCNFYNKRVVDIFGQKISSLPTSITIGIKETNAMLEDAEKYYAKGFRILK
                     IKTGISVEEDISRLHYLTEGFGKRMKIRVDANSGYDLQQLEQFLSATEKLPIELIEQP
                     LPPANNAALLNLPESVRKKLVADESLTDYESAEALAIQPITYGVFNIKLMKAGGILAA
                     HQIAGLAAQQNIDLFWGCNDESLISITAALHIAYACPNTRYLDLDGSLEVAEQLVTGG
                     FKIINGEMVITGNSGLGVRFKD"
     gene            complement(3399..4454)
                     /locus_tag="PLB46_02350"
     CDS             complement(3399..4454)
                     /locus_tag="PLB46_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HRF75383.1"
                     /translation="MSTKPKAIVLTGSILHESDAKTAHGLIRGTERFEIVAVIDTQHA
                     GKDAGEIIDGVFRNIPVYVDVNTAIQAINNIEYCIIGIATVGGVLPEAFLPIIETCIK
                     HKMSIVNGLHEYLSDNKHISSLAAEHQVNLIDVRKPKAKKDLHFWNGSIHQVKANIIA
                     VIGTDCALGKRTTCRLILQACQKHNINAQMIYTGQTGWMQGGKYGFIFDSTLNDFISG
                     ELEHAIVSCYNETSADVILIEGQSALRNPSGPAGAEFLVSGNAKQVILVHAPKRKHYE
                     HTPLWGEIHDVPSEIALIKMFGAEVIALALNTEDCSKDEALAFQQEYAAVLKIPVLLP
                     LQQGVEKVIPIIQGSIK"
     gene            complement(4465..6978)
                     /locus_tag="PLB46_02355"
     CDS             complement(4465..6978)
                     /locus_tag="PLB46_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF75384.1"
                     /translation="MRFFTTFLIIILSAVCSYAQPSAIVDEIDTKYSQLAATGRFNGT
                     VLYAEKGKVVYKKAFGVSNAQTGEPLTSTSAFNLASLSKQFIAAGTMVLYDEGKIQFS
                     DEVKKYLPTFPYEGITIQHLLQHTSGIPDYFDLYTQNRTPIDSLTNQGVLDLYIKYQP
                     ELEFATGSSWNYSNTNYVLIAMIIEKVSGQPLNKFMEEKIIKPAKLSDTYLYHIFMPT
                     VPENHVYGFQTVNGKTSLNDLWHVDGVYGDGNMYSSAEDLFKWEQLLYTNKIVKQTTF
                     AKALQPAPISDGTSYPYGFGWFIAKQDSIFMHTGGWAGFLNLICRDVVENRTLIVLSS
                     GSNYTGIPLGQSLFLNKPFEIPGTTLIENIELIDGTGTPQRKTSVRIEGDRILDIGNL
                     TPFKNEQVINGNGKVLAPGFIDTHSHVDRELNNTPNALAAISQGITTIVIGQDGESDP
                     IDSLKKWCSEIPKSVNIATYTGHATIRAMVMGKDDVYRPATQEEIDQMKQLLEAELAG
                     GSLGLSTGLEYEGGFYSTMDEVIQLAKTTAAGGGKYISHLRSEDIKLNDAIDEIITIG
                     YEAKIPVQISHIKIALKDDWGTATQIIAKLEMARESGIDITADCYPYTYWNATIRVLF
                     PNKDFTSMEGARYATTHLFDPSGSIMVRYKPNPEYAGKSISEIAAMRNESNEATLLYL
                     VAAAEAYGKLHPEDDYIEAIMGKSMSDEDIIPFLTWTHTNVCSDGANGGHPRGYGAFT
                     RVLGHYIREQQIMGLPTAINKMTALGASHVGIKERGIIAPGYFADLVLFDPELVKDNA
                     DIENSKALSDGILMVWVNGEIVYQDKSATNRFPGVFLSK"
     gene            7179..8888
                     /locus_tag="PLB46_02360"
     CDS             7179..8888
                     /locus_tag="PLB46_02360"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRF75385.1"
                     /translation="MKKYFTLLTGFALMIATADGQIFTKITTGDLVNTPSDSRSCNWV
                     DINNDGFQDVFISNGKAGGENNMLYINQGDGNFTTVTGDPIVMDNQPSDGATWADMDN
                     DGDIDAFVVNWYGKNNMLYSNNGDGTFEQITDGIPVNDFGYSETASWGDYDNDGYVDL
                     YVTNSDSIKKNYLYHNDGSAFSKITEGDITNEAYISRCANWIDVDGDNDVDMFVTNEF
                     NQQENLYLNNGDGTFTKKTSDPLVLNGGKTMSASWGDVDNDGDFDVFLANDGANNALF
                     YNDGAGNFTKATTDIVSNDGGNSFGSNFADIDNDGDLDLFVTNSFWGTMWNNFLYLNN
                     GDGTFTKNTTDVTATDMGWSYGNAFGDYDNDGNMDLVVANCYDAAQNNNLYNNNGNDN
                     NWVTISCVGTMSNKSAIGAVVKVLATINGEPTWQMTQISGQTGYCGQNMLPAHFGLGD
                     ATIIDSVYIYWPSGYVDITTDLPINQFATIAEGTYGNAVSTIVKNELQIVPNPADTFI
                     NLGVNELADIQIYNLSGQIVVQYIQYANENLNISAIETGNYIVVINTISNIKMAGRLV
                     VTR"
     gene            complement(9026..9634)
                     /locus_tag="PLB46_02365"
     CDS             complement(9026..9634)
                     /locus_tag="PLB46_02365"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRF75386.1"
                     /translation="MNNSSLFDLTKPIVLSTSVVELRPLQEADYALLLPFALNEPELW
                     TYSLVSAAGEAGLKNYLQIALHAKNQLREYPFIVFDKRTQQYAGSTRYYDIQLPMQTL
                     QLGYTWYGKQFQGTGLNTHCKYLLLANAFENMGIERVEFRADANNAKSIRAMKKIGCI
                     EEGILRSNTPKLSGGRRDSIVLSILKQEWIEKVKPMLEEKIK"
     gene            9767..>10478
                     /locus_tag="PLB46_02370"
     CDS             9767..>10478
                     /locus_tag="PLB46_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75387.1"
                     /translation="MVVALFIACMRVGNSQVIDTLNTQKEFILPSNSSNIGVSANLAS
                     YKIKGATTLSNVGIEPAISFAAYYNLNKLVTHGWYLQTQYAWADNIRNQASNAFGFEG
                     GYRASLLIAPFGLITGVGYKSTIYDFSNITPNQNFWLTQIYIPAEVYWSKLLPSNNAI
                     LVQLSIPIAELVSYSEATFRNPVSSANAEPLTFSLIHSDFEAALPFVDFFGSKLGLAY
                     VKTLSKTKALKLAYVFQYY"
     assembly_gap    10479..10488
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10593..10988
                     /locus_tag="PLB46_02375"
     CDS             10593..10988
                     /locus_tag="PLB46_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75388.1"
                     /translation="MKSNRHFFAVVILLMVSAFTYAQDAGIPNGSYKATQLASTGKPH
                     IITMESTTIDGVSHISYTETIDGVEVCSAKLVYTATESADRYLYKSTVAGTKDCTNGA
                     DMHFIVLDASGTAINMQRGSGRVFSLLKQ"
     gene            11204..11749
                     /locus_tag="PLB46_02380"
     CDS             11204..11749
                     /locus_tag="PLB46_02380"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HRF75389.1"
                     /translation="MKQTLITLFLLFSMNTILSAGNNPHVAALNIPVQSFDAIRVEKG
                     ITVIILQGDECGVKVADQQDANAINIRCKGAQLRISKKIFRKHTTVYVTSDQLTKIRA
                     CYGAQVIANDISGEQLELIVDENAVVDLEADVELTKSTNRNGLINITGDYQAEKGLYD
                     YNGRYSVIYTHSKNNLTENIK"
     gene            complement(11797..12237)
                     /locus_tag="PLB46_02385"
     CDS             complement(11797..12237)
                     /locus_tag="PLB46_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HRF75390.1"
                     /translation="MDITINTPALLFPAISLVMLAYTNRFMALSTVVRKLHDEYQTNT
                     SNNNLQLQIKNLQYRIKLVRRMQFFGVTSFLLAIMCMYTIYQDNFDLAHITFAASIIS
                     FSISLIISLIEIMQSTRALELELSDMEGMEGDNLVDYIRKKFDK"
     gene            complement(12286..>14664)
                     /locus_tag="PLB46_02390"
     CDS             complement(12286..>14664)
                     /locus_tag="PLB46_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75391.1"
                     /translation="TFTSGDDKLSLHLNIYSEQDSKNQPLDQTIDSEQRNILSSVGDS
                     LNQAFSSGIDSIAFDASRIMYKMIDTLGFDSVFVYSTNPDSAKYALSFTELGSNNGNY
                     ILTTTSANGRVYQWVAPIAGVPQGSAEPVILLIAPKKSTMVALGGSYKLTPNHTIGSE
                     LAFTNNDVNTFSDIDNSDNAGFAYRGSYNYNKSISATTKLQTQANYEFAAANFAPPER
                     YRPVEFNRDWNIFSTEKTNEHYANAGFTISGSEKWNLGFQSSAFIRENVYTGFLQALN
                     GGFTTTKWNLTAGGSFLQSSDDSNQAVFFRPKASIIRTFPSIKQWKTGILYEGEHNAI
                     SMQGDSLLSNSFYYDQLQYFIGSSDTAKTSIKGEIITRLDKLPLNGKFTNVTQGNTYS
                     LNGAFNKKINNKYSWQLTYRTLSILDTSLTLLNPEKSLLGRLQNSFTVKKGLVTGDLF
                     YELGTGQEPKRTFTYVEVEPGLGVYTWNDYNANGTQELDEFEIAVFADEANFVQVITP
                     TDEYIQSNLTNFNYALGLNPKVIWHDSKGFEGFIGKFAAQSSLQLSRKVLDDQSLNAY
                     NPFSEIADSLLVSSNVYWLNTLFFNRAGTKFGIDYSYLRNFNKIVITVGPESRGKDEH
                     KIIFRYKIAKPITVNGTLIKGNKSLVSDAFTNKTYFIPYYSAEPKITFIQNSVFRTTA
                     FYQYTTSKNNEGAETLISNEFTLDVRYNVVAKSTVSAKFSYIEIAFEGIEDTPVGYAM
                     LEGLNNGNNLLWNLTLDRKLSQLLQLTASYEGRKTGDNPITHLGRLQMRAIF"
BASE COUNT         4623 a         2653 c         2518 g         4860 t
ORIGIN      
        1 aaattatgta tgcccgaaat ggtcggtaac tgtttttgcc ttacaaataa ccaaacccga
       61 actttaccat gacggttata aaaaaccaac atcccgctgt gttgctatac aaattgcttt
      121 tgttttacat ttacttcaaa ttattccaaa aatctggtgc aacctttttg aacctcctcg
      181 ttatttaggt atacgcactt atggaaatct caataacaaa gtcgaaaatg gggtcatcaa
      241 aggcagctac ttctacacat tctccgctat ccctggagga tatgtacaag gtgtttgcaa
      301 aaaagatgat caacctgagt gcccggattg taaataatcc tattactgcg caggatattg
      361 ttcaggatgc atttatttcc ggtcatgccc atattcatca atttaccacc gaaaccgatt
      421 ttggcaattg gttaaaaagg gtagtggtaa ataaatcaat cgaccagctt cgaaaaaaat
      481 ctaatgccac ttttgaaatt gatgaaacaa cacatttggt tgctgagaca gatgaaataa
      541 gtgaagaacc ggaatatgat attccgaccg ttttgcaatc gatagccgaa ttacccgatg
      601 gttaccgttt ggtttttaca ctatttgtat ttgaacaata tagccataac gaaattgctg
      661 cagcattaaa tatttcggaa ggcacttcaa aatcacaata caatcgtgcc cgaaaaaaac
      721 tcattcaaat tattcaatct aaacagatta aatcatgagc aaattacgta tcgaagattt
      781 tatcttacaa aataaggatg cgttcgatat tattgaacct gctccaattg ctgctaccgg
      841 catactcagt agcacgacta aaattgtttc ctcatccatt aaatcaatta caatgaaagt
      901 tttagctata atttctatta ctactgttac tgtaatcagt tacttaactt tttctaatcg
      961 agaaaacact tctgctccaa caacaaaagg tggagaacaa actgttcaag catctactat
     1021 gaacgtatcg gaagaaccac caacaacaaa tcaaacatca gccatctacc cgggccacga
     1081 aaatccaatt acacatctgg aaaaacatag tccgttgact attttagatg aacaaataga
     1141 acctgacccg gaaataataa ataccaccta tatacaacaa acgtgggata gtattcaaat
     1201 gcgtcaaccg gaaacatata gttgggttcg aaaagggaaa agtgtaacct taaatattga
     1261 tactctgttt accggaatta cgcatgttga aataaatacc aattcatttg atgtaatcgc
     1321 tcaaggacaa acaggttcac ataatgtaaa tgtaaaggtt gaagcagaaa ataaaattaa
     1381 aggtatcgtt attaacgacc ctaaaattga agtagtttgt acgcttgaag gttccgtatt
     1441 aaaaattaaa gctttgtttg gagggagttc aggaattggg actttcgaat cggacggaac
     1501 acttacagtc aacgtgccgg aaacagcaac cattaaaata aatcacgact atggtaattt
     1561 agatgtcagt aatttaatta caaatttgtt tgatgtgaat gccagttcca gcaatgtgaa
     1621 aattaacaac ataactgcac caataaatgt acgtgttgat tacggtaatg tgcaactaga
     1681 taaaacagta ggtgcattaa acatggattt gggttcatgt aacgtaacct gcggaaatca
     1741 aaaaggtgat gtaaaaatcg aaagtgatta ttgtaattta agtatgggtg gaattcaagg
     1801 taatatcaat ggaaagctca attcagggaa agttgatatc ctcgatatta ccggaaatat
     1861 taagtttaat gctgattata ctaatattaa aatgaacaat gttaaagggg atttaagtat
     1921 ggtaacaaat tccgggaata ttgttggcga aaaaattgaa ttgatttctg cagcaaattt
     1981 caatacggaa tacggaagtg ttgatatcga ttttgttaat gatattgata aaatgagttt
     2041 cgatttaaaa acatcttacg gtaacatcca gttgcaagat ggtgttaacg cccgcaaaac
     2101 acagggagaa ttattaattg agcgtggagg aattaaagtt agctctacga cagatagcgg
     2161 gaatatcaag ataaaataag acagactaac aaacctgtta ttacaatgtc aatttaagtc
     2221 ttaaaagggt ttaggccgga cttaaacaag aatttgtaat aacaggtttt atttacttat
     2281 acaaattttg aattgactta gcgcctaatc agaaatcgtg gaattaaagt cctaatcttt
     2341 aaatctaact cctagaccgc tgtttcccgt gataaccatt tcaccattta ttatcttgaa
     2401 accacctgtt actaattgct ctgccacttc caaactgcca tctaaatcca aatacctggt
     2461 attgggacaa gcatacgcaa tatgcaaggc agcagtaata ctgattaaac tttcatcatt
     2521 acatccccaa aataaatcga tattttgttg tgccgctaaa ccggcaattt ggtgtgcagc
     2581 taaaatgccg cctgctttca tgagtttgat attaaaaaca ccataggtaa ttggttgaat
     2641 agctaatgcc tcagcacttt cgtaatcggt tagggactca tccgctacta atttttttct
     2701 aaccgattca ggtaaattta aaagtgcagc attattagct ggcggtaagg gttgttctat
     2761 aagttctatc ggtaactttt cagtggcact taaaaattgc tccagttgtt gcaaatcata
     2821 tcctgaattc gcatcaaccc gaattttcat tcttttgcca aaaccttctg ttaaataatg
     2881 taatctgcta atatcttcct caaccgaaat gcctgttttt atttttaaga ttctaaatcc
     2941 tttggcataa tacttttcag catcttccaa catggcgttt gtttctttaa tgccaatggt
     3001 aatgctggtt ggtagagatg aaattttttg gccaaaaata tccaccaccc tcttattgta
     3061 aaaattacaa taagcatcta ataatgcaat atcaattgct gcaattgttc ccggcatatg
     3121 agggaattgt tgcctgcatt gcgcaattat ttgaggataa tcctcaatat ttttacctac
     3181 taattcctga ataaaatcgc tttgtaaatt ttgtaaggtt aaagcagggg tttcacctat
     3241 aacttcagga aatgggttag aagcgccaaa gccttgtatc ccattatcga gcgtaacaat
     3301 aaaataaatt atttctgtat ctactatcga ctggtaagca attgcatagg gttgatccaa
     3361 cggaatgcgt tctaaaaaag cccttaccga gtgaattttc atttgattga tccttgtata
     3421 attggaataa ctttttctac accttgttga agtggcaaca ataccggtat ttttaaaaca
     3481 gctgcatatt cttgttgaaa tgctaatgct tcatccttag aacagtcttc ggtatttaaa
     3541 gcaagtgcaa ttacttctgc accaaacatt tttattaacg caatttctga tggcacatcg
     3601 tgaatttcac cccacaatgg tgtatgctca taatgcttgc gttttggcgc atgcactaaa
     3661 attacctgct tcgcatttcc tgaaactaaa aattcagctc ctgcaggccc acttgggttg
     3721 cgcaaagcag attgaccttc tattaaaatt acatccgccg atgtttcatt gtaacaactt
     3781 acaattgcat gttctaattc gccggaaata aaatcgttta aggtagaatc gaaaataaat
     3841 ccatatttac caccctgcat ccaacctgtt tggccggtat aaatcatctg tgcattgata
     3901 ttatgttttt gacaagcctg cagaattaaa cggcaagttg tccttttacc taatgcacaa
     3961 tcggtcccaa ttacggcaat aatatttgcc tttacctgat gtatgctgcc attccaaaaa
     4021 tgtaaatctt ttttcgcttt tggctttctt acatcaatta aatttacctg atgttcagct
     4081 gctaatgaac tgatatgttt attgtcagac aaatattcat gtagtccatt aacaatagac
     4141 atcttatgtt taatacaggt ttcaataatg ggtaaaaaag cttctggtaa aacaccacca
     4201 acagttgcaa tgccaataat acaatactca atattgttaa tcgcttgtat agcagtattc
     4261 acgtcgacat acacaggaat atttctaaaa acaccgtcaa ttatttctcc ggcatctttt
     4321 ccagcatgct gagtgtcaat aactgcaaca atttcaaacc tttctgttcc tctgataagg
     4381 ccatgcgctg tttttgcatc cgactcatgc aaaatgcttc ctgttaaaac aatagctttt
     4441 ggttttgtac tcacgaatac taatttattt gctcaaaaat acacccggaa aacgattggt
     4501 ggctgattta tcctgataaa ctatttcacc atttacccac accattaaaa tgccgtccga
     4561 caatgcttta gaattttcaa tgtcggcatt gtcttttact aattcagggt caaataaaac
     4621 taaatctgca aaataacccg gtgcaataat tcctctttct ttaatgccaa catgtgaagc
     4681 tcccaaagct gtcattttat taatagcggt tggcaatccc ataatttgtt gctccctgat
     4741 ataatgtcct aatactcttg taaatgcacc atatcctctt ggatggccac catttgctcc
     4801 atctgaacaa acattggtat gcgtccaggt taaaaatgga atgatatctt catcactcat
     4861 actttttccc attatagcct caatataatc atcctccgga tgtaatttac catatgcttc
     4921 tgctgcggca actaaatata ataatgttgc ttcattactt tcatttctca ttgctgcaat
     4981 ttcactaata gatttacccg catattctgg attaggttta taacgaacca taatagaacc
     5041 acttgggtcg aataaatgag tagttgcata tcgtgcacct tccatgctgg tgaaatcttt
     5101 gttgggaaat aaaacacgaa ttgttgcatt ccaataagta tatggataac aatctgcggt
     5161 tatatcaatc ccggattctc gtgccatttc aagtttagca ataatttgtg tggcagttcc
     5221 ccaatcgtct tttaatgcaa ttttaatatg cgatatctga acaggaattt ttgcttcata
     5281 tccaatagtt attatttcat ctattgcatc atttaattta atatcctcgc tgcgtaaatg
     5341 actgatatat tttcctccac ctgctgctgt tgttttagct aattgaataa cctcatccat
     5401 cgtagaataa aatccgccct catattctaa accggtagaa agtccaagtg aacctcctgc
     5461 taattcagct tccaataatt gtttcatttg gtcaatttct tcctgtgttg ctgggcgata
     5521 aacatcatct ttgcccatta ccattgcacg aattgttgca tgcccggtat aggtggcgat
     5581 atttactgac ttaggaattt cgctacacca ttttttcaaa ctatctatcg ggtcactttc
     5641 gccatcctga ccaataacaa ttgtagtaat tccctgacta attgcagcaa gtgcatttgg
     5701 tgtattattt aattcacggt ccacgtgact atgtgtatca ataaatcccg gtgctaatac
     5761 tttgccattg ccatttatta cttgctcatt tttaaatggc gttaagttac cgatgtctaa
     5821 aattctatcg ccttcaatgc gcacagaagt tttacgttgt ggtgttccgg tgccatctat
     5881 aagttcaata ttttcaataa gtgttgtgcc cggtatttca aatggtttat tcaaaaataa
     5941 tgattggcct aatggtatac ctgtgtagtt acttccactt gataacacaa ttaatgtgcg
     6001 attttcaact acatcgcgac aaattaaatt taaaaatcct gcccatccac cggtatgcat
     6061 aaaaatgctg tcctgtttag ctataaacca gccaaaccca taaggataag aggtgccatc
     6121 acttatagga gcaggttgta aagcctttgc gaatgtggtt tgtttaacaa ttttattggt
     6181 gtataataat tgttcccatt taaataaatc ttctgcagat gaatacatat ttccatctcc
     6241 ataaacgcca tcaacatgcc ataaatcgtt taggcttgtt ttaccattta ctgtttgaaa
     6301 accatacacg tgattttcag gcacagtcgg cataaaaata tgatacaaat acgtatcaga
     6361 taattttgca ggttttatta ttttttcttc catgaattta tttagcggtt gtccactaac
     6421 tttttcaata atcatggcaa ttaatacata attagtattg ctgtaattcc aactacttcc
     6481 tgttgcaaat tccaattctg gttgatattt tatataaagg tcgagaacac cctgattggt
     6541 taaagaatca ataggcgtac gattttgggt atacaaatca aaataatcag gaatcccact
     6601 ggtatgttgt aataagtgtt gaatggtgat accctcgtat gggaaagtag gtaggtattt
     6661 tttcacctca tcgctaaatt gaattttacc ttcatcataa agcaccatag tgcccgcagc
     6721 aataaattgt ttgcttaaag aggccaggtt aaatgctgat gtactagtta atggttcacc
     6781 tgtttgtgcg ttggataccc caaatgcctt tttatatact accttacctt tttccgcata
     6841 caatactgtg ccgttaaacc tcccggttgc ggccagctga ctatacttgg tatctatttc
     6901 gtcaacaatt gcagatggtt gggcataact acatactgca cttaaaataa taataaggaa
     6961 agtggtaaaa aatctcattt gtttggaata tgctcaaaat taagtgtttt ctctatgtgt
     7021 taagatttgc ccgttaacaa aacttgccgc tgtaatctcc aattcatcac ctttatggac
     7081 taaaacagtt aaaaacctgt ttaaaaatcg ttaaatgact ttaaaatgaa cagtataccc
     7141 aaaccacctt tttatcttgc atgcacaaca tattgacaat gaaaaaatac tttacccttc
     7201 ttactggttt tgccctaatg attgctactg ctgatgggca aatttttaca aaaattacaa
     7261 ccggtgactt agtaaataca ccttccgatt cgcgaagctg taattgggta gatattaata
     7321 acgatggttt tcaggatgtg tttatttcca atggcaaagc aggtggtgaa aacaatatgt
     7381 tatatattaa ccagggtgat ggcaatttta ccactgtgac tggtgaccct atagtgatgg
     7441 ataatcaacc ttcggatggt gctacttggg ccgacatgga taacgatggt gacatcgatg
     7501 cttttgtggt aaactggtat ggaaaaaata atatgttgta ttccaataat ggtgatggca
     7561 cgtttgaaca aataacagat ggcattccag ttaatgattt tggatattca gaaactgctt
     7621 cctggggcga ttatgataat gatgggtatg tagatttata tgtaacaaat tcggacagca
     7681 tcaaaaaaaa ttatttatac cataatgatg gctctgcatt ttcaaaaatt accgaaggcg
     7741 atataacaaa tgaagcttat atttctcgtt gtgcaaattg gattgatgtt gatggcgaca
     7801 atgatgtaga tatgtttgtc accaacgaat ttaatcagca ggaaaattta tatctcaata
     7861 atggcgatgg cacttttaca aaaaagacaa gtgacccact ggtattaaat ggcggaaaaa
     7921 ccatgagtgc aagttggggt gatgtagata acgatggtga cttcgatgtt tttttggcaa
     7981 atgacggagc aaataatgca cttttttata atgatggagc aggtaatttc actaaagcta
     8041 ctactgatat tgtaagcaat gatggtggaa attcattcgg aagtaatttt gctgatatcg
     8101 ataatgatgg tgacttggat ttatttgtga caaattcttt ttggggtacg atgtggaata
     8161 attttttata cctcaataat ggtgatggca catttacgaa aaatactacg gatgttactg
     8221 ctacagatat gggctggagt tatggtaatg cttttggcga ctatgataac gatggcaaca
     8281 tggatttagt ggttgctaat tgttatgatg cagcccaaaa taataatctt tataataata
     8341 atggtaatga taataattgg gttactattt catgtgtagg caccatgtct aacaaatcgg
     8401 caataggtgc ggttgtaaaa gttttagcta ctattaatgg cgagccaaca tggcaaatga
     8461 ctcaaataag cggacaaaca gggtattgtg gtcaaaacat gttgcctgcg cattttggat
     8521 tgggtgatgc aacaattatt gatagtgtat atatttattg gccaagtggg tatgtagata
     8581 tcactactga tttacctata aatcaattcg ctactattgc agaaggcact tatggaaatg
     8641 ctgtttcaac tattgttaaa aatgaattac aaatagttcc aaaccctgct gataccttta
     8701 ttaatttggg ggtgaatgaa ttggctgaca tccaaatcta taatttaagc ggacaaatag
     8761 tcgtacaata tattcaatat gcaaatgaaa atttaaatat ttccgccatt gaaaccggta
     8821 attatattgt tgtaataaat accatttcta atataaaaat ggctggtaga ttagttgtaa
     8881 ccagataata caatttattt accttagtca ataatcaatc ttacagcatg atgatttgag
     8941 cgcaatttaa tacttttaat tttggatgtt ggaagttgca atttctgttg cgaaaataat
     9001 aatcattcct gcagtatttt agaatttatt ttattttttc ctccaacata ggttttacct
     9061 tttctatcca ttcctgtttt aaaatactta gcacaatact atctcgacgt cctccactta
     9121 attttggtgt gttacttcgt aatatgcctt cctcaataca accaattttt ttcattgcac
     9181 gaatactttt tgcattgttg gcatcagcac gaaattcaac ccgctctata cccatgtttt
     9241 caaatgcatt tgctaataat aaatatttac aatgtgtatt taagcctgtc ccttgaaatt
     9301 gtttgccata ccatgtatat ccaagttgta aagtttgcat gggcaattga atatcataat
     9361 atcttgtgct gccggcatat tgttgcgttc ttttatcaaa tacaataaat ggatattcac
     9421 ggagttgatt ttttgcatgt aatgcaattt gtaaataatt tttcaaccct gcctcaccag
     9481 ctgctgaaac tagtgaatat gtccataact caggttcgtt aagtgcaaaa ggaagtaata
     9541 acgcataatc ggcctcctgt agtggcctca attctacaac tgaggtagaa agcacaatag
     9601 gttttgttaa atcaaagagg gaggagttgt tcatacataa atacttattt gcaaaattat
     9661 catttgcctt tataatttag caacgtatgg ggaattttat atatttgaaa tcgtgaggca
     9721 accttatata ttacactgtg gttacacatt taagtatcat ttatttatgg tggttgcttt
     9781 atttattgct tgtatgcgtg ttgggaattc gcaggtcatt gatacattaa acacccaaaa
     9841 ggaattcatt ctgccttcca atagctccaa tattggggta tcggcaaatt tggcttccta
     9901 taaaattaaa ggtgcgacaa ctttatccaa tgttggtatt gagccggcta tttcgtttgc
     9961 tgcttattat aacttaaata aactggttac ccatggctgg tatctgcaaa cacaatatgc
    10021 gtgggctgat aatatcagaa accaggcttc aaatgcgttt ggatttgaag gagggtaccg
    10081 ggcttctctc ttaatagctc catttggatt aataaccggt gtaggatata aatcaacaat
    10141 ttatgatttt agtaacatca ccccaaatca aaacttttgg ttgacacaaa tctacattcc
    10201 ggcagaagtt tactggtcga aattattgcc atccaacaat gccattttag ttcaactttc
    10261 cattccgatt gccgaattgg tgagttattc tgaagctaca tttagaaatc ctgtatcgtc
    10321 agctaatgca gaacctttaa cttttagtct aatacatagt gattttgaag ccgcattacc
    10381 ttttgtagat ttttttggct caaaactggg gcttgcctat gttaaaacac tatccaaaac
    10441 caaggctttg aaattggcct atgttttcca atactatcnn nnnnnnnnga tgatggtttt
    10501 gccttggttt accagttcta aaaggcaccg ttttttggag aaatcacgca aatggtttat
    10561 ttttagcatc agaaacaaca aaacgtattc atatgaaatc aaatcgccac ttttttgccg
    10621 ttgttatttt attgatggta tcagcgttta cctatgcgca ggatgccggt atacctaatg
    10681 gctcctataa agctactcaa ctggcttcaa caggtaaacc acatatcata acaatggaaa
    10741 gcactacaat tgacggtgta agtcatattt cttacactga aactattgat ggtgttgaag
    10801 tttgctctgc aaaattggtt tacactgcaa ctgaatctgc agaccgatat ttgtataagt
    10861 ctactgtagc aggaacaaaa gactgcacta atggagctga tatgcatttt atcgtgttag
    10921 acgcttcagg tactgctatt aatatgcaga gaggctcagg tagggttttt tcgcttttaa
    10981 aacaataaac ctatttaata atattttacc ccttggcgtt tgttcgtcaa ggggtttttt
    11041 attgccaatt gttcataaag aaaaactgct tactaagtga ataaccgatt tcacacaaaa
    11101 tttagtgcac ttcgctcaaa cccgtttttt tgttgttggg aaagaaaata cttttgaatt
    11161 gttaattaat tgaaaatatt tatacgcaca atatttacct gttatgaaac aaacgctcat
    11221 tacccttttt ctgctatttt ccatgaatac catattaagt gccggcaata atccccacgt
    11281 tgccgcactt aatattccgg tgcaatcatt tgatgctatt cgtgttgaaa aaggcattac
    11341 tgtaattatt ctgcaaggcg acgaatgcgg agttaaggtt gcagaccaac aagatgcaaa
    11401 tgcaataaac attcggtgca aaggtgccca actccgaata agtaaaaaaa tattcagaaa
    11461 acataccaca gtttatgtta ccagcgatca attaaccaaa attcgtgctt gttatggcgc
    11521 gcaggttatt gctaacgata ttagtggcga acaactcgaa cttattgtag atgaaaatgc
    11581 agttgtagat cttgaagcag atgtggaact gactaaatcg actaacagaa atggattaat
    11641 caatattaca ggcgattatc aggccgaaaa aggtttatac gattacaatg gaagatattc
    11701 agtaatatat actcattcaa aaaacaatct tacagaaaat attaaataat attatatcat
    11761 gcagatttcc aggaggttca catttgtgag ccttttttat ttgtcaaatt ttttgcggat
    11821 atagtctacg agattatcac cttccattcc ttccatgtca gacaattcta actccagggc
    11881 tctggtactt tgcataattt caattaaaga aataattaaa cttatcgaga aactaatgat
    11941 agacgcagca aatgtgatat gagccaaatc gaaattatcc tgatatatgg tatacataca
    12001 cataattgct aataaaaaag aggtcactcc aaaaaactgc atacgacgaa ccaactttat
    12061 acgatattgg agattcttaa tttgtaactg taagttgtta ttggatgtgt tagtctgata
    12121 ctcgtcatgt aactttctta ctaccgtact caaggccata aatctgtttg tataagctaa
    12181 cattaccaaa cttattgccg ggaataacaa agccggtgtg tttattgtga tatccataat
    12241 gcaaattaaa ctaattaata ggaattggca gtttttcttc tttatttaaa atatggctct
    12301 catttgtaat cggcccaaat gggtaatcgg gttgtcgccg gttttacggc cttcatagct
    12361 ggctgttaat tgcaacagtt gtgataactt tctgtccaat gttagattcc ataacagatt
    12421 gtttccattg tttaatccct ccaacatagc gtaacctaca ggtgtatctt caattccctc
    12481 aaatgcaatt tcgatatacg aaaacttagc tgaaactgtg cttttggcaa caacattata
    12541 tcttacatct aatgtgaatt cattacttat taatgtttct gctccctcat tatttttact
    12601 ggttgtgtat tgatagaatg cagtggttct aaaaacactg ttttgaataa aagtaatttt
    12661 tggttcagcc gaataatatg ggataaaata ggttttgttg gtgaaagcat ctgataccag
    12721 cgatttattt cctttaatta aagtaccatt aactgttata ggtttggcaa ttttatagcg
    12781 gaatattatt ttatgttcat ctttccctct gctttcaggg ccaactgtta tgacaatttt
    12841 attgaaatta cgtaaataac tatagtctat accgaattta gttcctgctc tgttaaaaaa
    12901 taaggtattt aaccaatata catttgaact caccaacaaa ctatcggcaa tttcagaaaa
    12961 tggattatat gcattcaacg attgatcatc caaaacttta cgtgaaagtt gcagtgatga
    13021 ttgtgcggca aattttccaa taaacccttc aaaccctttg ctatcgtgcc aaataacctt
    13081 tggatttaaa cctaatgcat aattgaaatt ggtcaagtta ctttgtatgt actcatctgt
    13141 tggtgtaatt acctgaacaa aattggcttc atcggcaaat accgcaatct caaattcatc
    13201 taattcctgg gtgccatttg cattataatc attccatgta tatacgccta atcccggttc
    13261 aacttcaaca tatgtaaatg tacgtttagg ttcttgtccg gtgcctaatt cataaaataa
    13321 atctccggtt actaaacctt tttttactgt gaaactattt tgtaatcggc ctaataatga
    13381 tttttcagga tttaataagg ttaatgaagt atctaaaatg gataaggttc tgtaagttag
    13441 ttgccatgag tatttattat ttattttttt attaaatgca ccatttaagg aatatgtatt
    13501 gccctgtgta acatttgtga attttccgtt taatggtaat ttgtctaagc gggtaattat
    13561 ttctccctta attgaagttt ttgcggtatc agaacttcca ataaaatatt gtaattgatc
    13621 gtaataaaag gaattactta gtaatgaatc accctgcata gaaattgcat tgtgttcacc
    13681 ctcatacaaa atgcctgttt tccattgttt aatggaaggg aatgttctta ttatacttgc
    13741 tttaggacga aaaaacaccg cttgattact atcatctgaa gattgtaaaa aactccctcc
    13801 ggctgttaag ttccatttag tagttgtaaa tccaccattc agcgcttgta aaaaaccggt
    13861 ataaacattt tcgcgaataa atgctgatga ctggaatcct aaattccatt tttcactacc
    13921 ggaaatagtg aagcctgcat ttgcataatg ttcatttgtt ttttccgttg aaaaaatatt
    13981 ccaatcgcga ttaaactcaa ctggacgata acgctcaggt ggagcaaaat tagccgctgc
    14041 aaattcgtaa tttgcctgtg tttgtaattt agtggtagca cttatacttt tattgtaatt
    14101 ataacttcct ctgtaagcaa atccagcatt atctgaatta tcaatatcag aaaatgtatt
    14161 tacatcatta ttggtaaatg ccaactcact gccaattgtg tgattaggtg tgagcttgta
    14221 acttccacct aatgctacca ttgtcgattt ttttggagct attaataaaa taacaggttc
    14281 ggcacttcct tgtggaaccc ctgcaatagg ggctacccat tgatataccc tcccgtttgc
    14341 ggaagtggtt gttagtatat aattcccgtt gttactcccc aattcggtga atgataatgc
    14401 atatttagcc gaatcgggat ttgttgaata aacaaaaacg gagtcaaagc ctaacgtatc
    14461 aatcatctta tacattattc gcgaagcatc aaatgcaata ctatcaatac ctgatgaaaa
    14521 agcttgattt aatgaatctc caaccgagga caaaatattt cgttgctccg aatctatagt
    14581 ttggtctaag ggttgatttt tactatcctg ttcgctgtaa atatttaagt gtaatgacaa
    14641 tttatcatct cctgatgtaa aggt
//
LOCUS       DAORKW010000028        14663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_50_length_14663_cov_5.453861, whole genome shotgun sequence.
ACCESSION   DAORKW010000028 DAORKW010000000
VERSION     DAORKW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14663
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50_length_14663_cov_5.453861"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="PLB46_02395"
     CDS             complement(<1..1445)
                     /locus_tag="PLB46_02395"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRF75392.1"
                     /translation="MKPILRNLLLTALSTATLLFTANTGYAQIEVDDSPTGIELAEAL
                     IGEGVTISDINLNCPTGASGFFDCIDCSVGIESGILLTSGLVTNAIGPNNFDGMGYAH
                     FTPGDPDLDAIPGIIGTGDACVLEFDLTVTSDTVRFNYSFASEEYPEYVDFFNDVFAF
                     YISGPGIVGIENMALIPGTTTAVTINNVNDEDYSEYYVNNGNGYNPPYNSDDYYIQYD
                     GFTTPLEAKRQVIPCETYHLKMVIADDGDNAYDSGVFIEAGSLSSPGVNLAYETEIDG
                     YPYIIEGCNDGSLTFELSFAPIDTFEVVINVGGTAIAVTDYDMQTTLLFLPGDTLLEI
                     PIIAPEDGIDEGIETIVISLEAGCVTGLEDSLVLYIYDAIPVEISPDTMICPGTDAVL
                     VASGGDTYSWTPEATLSDPSSSTTVASPTEETTYTVTSTLASCINTATTTVSIQESSA
                     YAGEDTTIYFGESAFLEATGGVEYTWLPILT"
     gene            complement(1605..2903)
                     /locus_tag="PLB46_02400"
     CDS             complement(1605..2903)
                     /locus_tag="PLB46_02400"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRF75393.1"
                     /translation="MIQLRSYATLAELFNDWFGTNMRFPVFSFGFFVGMAFLVAGWIL
                     YKELKRKKEQGLFKSTSIKVVIGKPASTSELIFNGIVGFLVGFKLGGMILNWSAFNDA
                     PQAYVFSGEGNILFGLLGAVALAYLKYREKKKEQLPEPKEKTITIFPHDRLGDIVMVA
                     AVSGILGAKIFSNFEEPNGWKDFIADPFGNFFNGLTIYGGLLLGALCVILYAKSKKIH
                     VLHLGDAVAPALILAYAVGRMGCQVSGDGDWGILNSAYVSQADGSVVAAQPGDFEKIL
                     EANAAYYEREFESINNVPSSHFEGPSFLPRSLFAQNYAYNVNNEGAYIQGCQGDWCGQ
                     LPVAVYPTPIYEIIMSLIIFGILWFLRKKLKIPGMLLALYVIFAGLERYFIEKIRVNN
                     VLEFMGIHATQATFISIIFISFGLGMMFVLWRMSKRTPQS"
     gene            complement(2915..4285)
                     /gene="mtaB"
                     /locus_tag="PLB46_02405"
     CDS             complement(2915..4285)
                     /gene="mtaB"
                     /locus_tag="PLB46_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRF75394.1"
                     /translation="MQKRSVALYTLGCKLNYSESSALGRQMEEAGFEIKGFHEPADVY
                     VVNTCSVTEHADKKCKKIVRQALKISPNARIVIVGCYAQLKPEEIVNIPGVDLVLGAA
                     EKFNLTTHILSLPPKSENNKGQFINGNISEVKQFNTAWSFGERTRTFLKVQDGCDYSC
                     SFCTIPLARGASRSNTISNVIENVNRIAENDVAEIVLTGVNLGDFGHLSGLEKEESFF
                     ELVQALDKVEKIQRFRISSIEPNLLQEEIIAFVAKSVKFVPHFHIPLQSGSDKILRLM
                     RRRYQRDLYASRVQMIKNVMPHCCIGVDVITGFPGETHEDFLTTYQFLNELPVSYLHV
                     FTYSERPNTFALNLPEPVPQKIREERTTQLRSLSEKKRRYFYHQFVSTTRPVLFEEET
                     DGVWMYGFTDNYIRVKTHYDPLLVNEVVEVQLTAIDADGDMLGEPIDKANSTILAHET
                     IQSLNK"
     gene            4448..5179
                     /locus_tag="PLB46_02410"
     CDS             4448..5179
                     /locus_tag="PLB46_02410"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRF75395.1"
                     /translation="MLIEHRGKCFDLSKPIDIGISLHDGTQLNCYSAPPFSSRPVVLG
                     DFVGDMQQGGLLNYKTVTLNPHGNGTHTECLSHVYDTPLTINQALKQFHFLAQLITLI
                     PHKTKENDLVLNTAHIKDKIEPGVNALIIRTLPNEKSKLTRNYNKTNPPYLEESLATY
                     LNKQGIFHLLVDLPSVDREEDGGKLAAHKAFWGIPNKLRPSATITELIYVPNEVKDGI
                     YLLNLQIAAFELDATPSKPVLYPQL"
     gene            5271..7508
                     /locus_tag="PLB46_02415"
     CDS             5271..7508
                     /locus_tag="PLB46_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRF75396.1"
                     /translation="MKKRLLTTILLAGIMVLGQAQISIADARILTPGTNVTVKGLVLN
                     GAELGDIRYIQDNTGNIAAFGATLLDGVLRGDSIQVTGPLFNYNSLLEISPVSSVTVI
                     NSGNPLPAPTSITTVSGYIESFEAKLVTVNAALFTETGTFSTAGSGTNYDITDAAGTA
                     QVRVVTSTNIDGTPIPTDEVSVTGIMSQFAPGGTGGYQLLPRDLADISTGGNPPVFST
                     GLTQTDITTSSFTVNFSTLNPGNTIIYYGLTTALGSVSSDAALVTTHAHNLTGLTAGT
                     IYYVQAASVSATNDTSFSAISAMATASNSSGQIKVWFNNTVDNSVSTGTNAIFVNGAM
                     DDTIIWYLDHAKYSVDLAIYNIDNVNNIITAINDAYSRGLDVRVVCDSGVDDFNYGLI
                     NIGPGKKKKSPPDGSTNADGALYGIMHNKFIVVDAISTDPDDAWVITGSMNFTDGQVK
                     LDKQNIIAIQDQSLARGYRLEFDEMFNGKFGPDKSNNTPHEFIIGGNRVELYFSPSDE
                     TETRMLEKINTADYDLHFAVFSFTRFGISYEIEDAVTERGVFAAGIYDQTDASDSTAV
                     NILEDALGDKFFNYSGSNLLHHKYLLIDANCSQSDPLVWTGSHNWSTSANSRNDENTL
                     VIHDSTTTNIYYQEFMQRYKEEGATTFVSEKCDFVGVEEHVVENTILLFPNPAQNELN
                     IYADNLADFELLYIYDLSGKKILSAAVNNESLININITDLSAGVYFIVIQNGETIYSK
                     QFVKK"
     gene            7529..9058
                     /locus_tag="PLB46_02420"
     CDS             7529..9058
                     /locus_tag="PLB46_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75397.1"
                     /translation="MKSTLYFISLILVSVQVFCQNCEPVVAYNFNNNTTDISVNDYNA
                     TLFNGASTVDDYLEIGYNDDDRAEIPADALNGISDFTIKFDFYLTGLNLSGAAPTNTI
                     IAGASATDESEFAISYQADIMAIVIALKGSGDVFPVELNAENWYCLTVTRAESIVSVF
                     INGTEVSSIDMTSSDLDIEFLEIGQELDCVSGCYATNQSLNGRIDNLSIYPCAIENSN
                     CEPYVAECDTLVYYKFDGTVLDEGIFANNGILNAGAAVSSNILEIDYNDNDYVEIPSS
                     AADGLDEFAISFNFYLNNLNEDGSSPTNTFIAGTDGIDMHELALSYESSSNAFEVAIH
                     EIGGIIPATILPGTWYCVTFYRKSDSVWIELDGIILPTVLSLPEGPLSISFLEIGQEL
                     DCAEGCFAENQSLNGKMDNLIIQGCSTIINCNPASVGIYSNQNSDVAITPNPASNWIT
                     INGVNLNSANISITNLQGQKLPLEILNNTLVNIAHLPEGMYFLIIESNGSVMVKRFIK
                     E"
     gene            complement(9139..10266)
                     /locus_tag="PLB46_02425"
     CDS             complement(9139..10266)
                     /locus_tag="PLB46_02425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF75398.1"
                     /translation="MVKKIAAVTFWFPTKEKPLSFPFIVEHLKAISKTQHLAFVLYIR
                     VEQSHKLYAQSISKYDYEGLKIVEIFVTSKFHKLLYQFPILLWPSIKKRIKTENFDQI
                     DVVHGMVVFPAGVLAYLIAKKINKPLVLTEHWSKTTKFLTSHLLSFIGIKAFKYAEKV
                     TFVSKWLQHEVCKVIPALVGKSVIVPNVVDGNLFHYTPKIQKPEMPIRFIAVASNWNA
                     DRDLFLIVNSLEQLVRKSGKSIELVLIGGGILYDEIKSLKWSYPILFSGFQSRQEIAN
                     QLNDSHYFLFSSKFETFSVVTAEALVTGTPVLVSDVAALPELVKQGYGFTTPNTIEGW
                     NNALEKLIETKFDNAKIAAENITIFNAENIAAAFEKVYEGI"
     gene            10316..11131
                     /locus_tag="PLB46_02430"
     CDS             10316..11131
                     /locus_tag="PLB46_02430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF75399.1"
                     /translation="MSNNQTPNFDPQAYWEKRLKSNTGLTGVGYTLVGEHYNKWAYKI
                     RKYVFNKIVTKNLKDQKNADILDIGSGSGFYIDEWLTYQPKSMTGIDITEIAVSRLSE
                     KYTDLTFHKLDISDLAATQLMFQQKFHAISCMDVLFHIVDDEKYKSTFQSVAQLLVNG
                     GYFIFSENFVHEQLSTLKHHKSRSKVEIEQLLAQNNFKIISRRPFMYLMNEPVDSNSF
                     ILKWLWKITAIICAKSALLGYIIGAITFPIEKLLVSLLKESPTSEIVIAVKLK"
     gene            complement(11255..12880)
                     /locus_tag="PLB46_02435"
     CDS             complement(11255..12880)
                     /locus_tag="PLB46_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75400.1"
                     /translation="MKILKLLIAFSLLLTVKIVDAQFIKSYDNGPEQGIRFAALVPVD
                     ETTTSNLYLAGSNEGYLIIGEIDHSGVLVWNKRINAKDTSMTINQMIKDSDGNLVLVG
                     TSIAGDLGKSFVVKFDPVIQDIVWFRKCTVNSFFWDVVELGPGGDYLVGGQETNNGTG
                     AGSDDITIKFKRASGAQSVITNLNKNMNESVEAVAYDPTSSSIYSTGRYELSTGGNNK
                     FRICINKFDITGTIDWTRSYIKSTATSGRFYSEDMLIDGEHIIVIGSGDDVSTSSNKY
                     FWFIKTDLSGEAVITRKIDVTGAFYDGVFAGIKKHESGYIVYGSLYQDGKYTNVCLYN
                     LDFDGNVVWAKSYPFRLRYPTTGLFSSSAMTIVGDDIFVVGEQVGDGGTVEGIFMKTS
                     ILTGEIGVCETEILNTSTVVTNYDNFETLESETVTLSYPSSFPGIKPLALNMSTVCSP
                     NDGKLVLTSSNSDSFIYPNPSNGFIYMNEIQGYNLDDKVLITDIAGKSVATCNVEQLQ
                     HGFSIANFAPGMYFVTVVNENTSATKSCSIVKE"
     gene            complement(12883..14343)
                     /locus_tag="PLB46_02440"
     CDS             complement(12883..14343)
                     /locus_tag="PLB46_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75401.1"
                     /translation="MKLKIVIIIALLGSMVQIGNSQSNFVGSGLALNFDGNSDNYYDL
                     GDNYNTLNFPLTFEAWLNQSEYSLYTPVFASDSYTSGNYYGFYIRFDPVGKLIFEIGN
                     GVGAGGAHRRGKITTSTVPLNEWVHIAIVATSVTDIKFYFNGVLQSAVSTDGTASNTS
                     IVHNSNKVNLGRYATVHRSDAFIGKIDEVRLWNVARTELDIRDKMCEKLTLSETGLIG
                     YWIVDENTTETTLQDYSVTGANGNEIGSVEKSNSGAPIGNASVFVYQTDYSGATLSLN
                     SMIGDKLKVNKILGTPYGIHLYRVDSYPIGDNGLINSPDMYYGVFPVDNAVDATYTMA
                     YTYAYSNGVSTVSNEENSKLFKRLDGSIDTWINQPAMLNIAGKKLTKKNFAGRTEIIL
                     NIPNPNGRSNNTVQDLIKDDQISVYPVPATELISITNLQKGSSLQIYDCNGRLVFSNI
                     CNNDYLHVNISQFEPGLYIIIENNISGSSITKFQKI"
     gene            complement(14340..>14663)
                     /locus_tag="PLB46_02445"
     CDS             complement(14340..>14663)
                     /locus_tag="PLB46_02445"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75402.1"
                     /translation="PNSSLVKKNCIGRGEHILNIPFVNEMKAEITPELEVFPNPSADY
                     FITKSLSTNPITVSDNQGKIIRHYTVFEVGELITLDLSRLPAGNYFVSQQVNGKNITS
                     KIVKQ"
BASE COUNT         4760 a         2812 c         2427 g         4664 t
ORIGIN      
        1 aatgttaaaa ttggcaacca ggtatattca acaccacctg tagcttcaag gaaagctgat
       61 tcaccaaaat atattgtggt atcctctcct gcataagcgc tcgactcttg tatgctaaca
      121 gtagtcgttg ctgtattgat acaagaagct aaagtagaag taaccgtata ggtggtttcc
      181 tcagtaggtg aagccacggt agtagaggaa cttggatcac tcaaggttgc ctctggagtc
      241 caagaatacg tatcgcctcc actagccact aaaacagcat cagtacccgg acaaatcatc
      301 gtgtctggtg aaatttcaac aggtatcgca tcgtaaatat ataatactaa actatcctct
      361 aaacctgtaa cgcatccagc ttcaagcgat atcactatag tctcaatacc ctcatctatg
      421 ccatcttctg gtgcaataat tggtatttct aacaaggtat ctcctggcaa aaataacagc
      481 gtagtttgca tatcataatc agtaacagca atggccgttc caccaacatt aataacaact
      541 tcaaaagtat caataggcgc aaaagataat tcaaaagtta aactaccatc attacatcct
      601 tcgataatat atggatatcc atcaatttct gtctcgtagg ccaaatttac acccggactt
      661 gataaggatc ctgcctcaat aaatacacca gaatcatatg cattgtcacc gtcatcagca
      721 atcaccattt tcagatggta agtttcgcat ggtataacct gacgtttagc ttctagagga
      781 gtagtaaaac cgtcatattg aatataatag tcatcactgt tgtatggggg gttataacca
      841 ttaccattat taacataata ttcggaatag tcctcatcat taacattgtt tattgttacc
      901 gcagttgtgg ttcccggtat caaggccata ttttcaattc ctacaattcc tggtccgctt
      961 atgtaaaatg caaatacatc gttgaaaaaa tctacatact caggatactc ttcagatgca
     1021 aaactataat taaaacgcac cgtatctgaa gtaacagtca agtcaaattc taatacacaa
     1081 gcatcaccag taccaattat acccggtatc gcgtctaaat caggatcacc cggtgtaaaa
     1141 tgagcataac ccatcccatc aaaattattg ggaccaatag catttgttac aagtcctgac
     1201 gttagtaaga tgccactttc aatacccaca ctacaatcta tacaatcaaa aaaaccagat
     1261 gcacctgttg ggcaattcaa atttatatcg gatatggtaa ccccttcacc gataagcgct
     1321 tcggccaatt caatgccagt aggggaatca tcaacttcaa tttgtgcgta gccggtatta
     1381 gctgtaaaca gcaatgtagc agtagataac gcagtaagga gtaaattgcg taaaattggt
     1441 ttcattgtta aaatatatgt ttaggttaaa cctaattata aaagcactct caaacttacg
     1501 aataatttaa caaatcacat taaattatag tagtttttaa ttgccgaata tgacatagca
     1561 gtgacacata tgccatcctt caacttgcga ttggcagggg tttattaact ctggggtgtg
     1621 cgttttgaca ttcgccacaa tacgaacatc attccaagcc cgaagctaat aaatataatt
     1681 gatatgaagg tagcctgtgt agcatggata cccataaatt ccaatacatt gttgacacgt
     1741 attttttcaa tgaaatagcg ctcaagccct gcaaatatca catacaaggc gagtaacata
     1801 ccgggaattt ttaacttttt tcgcaaaaac cataaaatcc cgaaaataat gagcgacatg
     1861 ataatttcat atatcggggt cggataaact gccaccggaa gttgaccgca ccaatcgccc
     1921 tgacaaccct gaatatacgc gccttcgttg tttacattat aagcataatt ttgggcaaat
     1981 agcgagcgcg gtaaaaacga aggaccttcg aaatgtgatg atgggacatt gtttattgat
     2041 tcgaattcac gctcataata tgcagcattt gcttccaaaa ttttttcaaa atcaccaggc
     2101 tgagctgcta ccacactacc atcagcttgt gaaacatagg cactgtttaa tatcccccaa
     2161 tcaccatcgc cggaaacctg acatcccatt ctaccaacag cataagccag aattaacgcc
     2221 ggtgcaactg catcgcccaa gtgtaataca tgtatttttt tactttttgc atacaatatc
     2281 acacataagg caccaagtaa taatccacca taaatggtta aaccattaaa gaaatttcca
     2341 aacggatctg caataaaatc tttccaacca tttggctctt caaaattgga aaatattttg
     2401 gcgcctaaaa ttcctgagac agcggcaacc attacaatat ctcccaatct gtcgtgtggg
     2461 aaaatagtaa ttgttttttc ttttggttcg ggtaactgtt cttttttctt ttcgcggtat
     2521 tttaaataag ccaaagcaac cgcgccaagc aaaccaaata aaatatttcc ttcacctgaa
     2581 aatacatatg cttgtggagc atcattaaat gcactccagt tcaaaatcat tccgcctaat
     2641 ttaaaaccaa ctaaaaaacc aactatacca ttaaaaataa gttcggaagt actcgccggt
     2701 ttaccaatta caacttttat gctggtcgat ttgaatagtc cttgctcctt ttttcttttt
     2761 aattctttgt ataaaatcca gcctgcaact aaaaatgcca tccccacaaa aaatccaaaa
     2821 ctaaatacag gaaaacgcat gtttgtgcca aaccaatcgt tgaataattc tgctaaagtt
     2881 gcgtatgaac ggagttgtat catttaatat atttttattt atttaaagat tggattgttt
     2941 cgtgtgctaa aatcgttgaa ttagccttat ctattggctc acccaacatg tcgccatcag
     3001 catcaatagc agtgagttgc acctctacta cttcatttac taatagtgga tcataatgtg
     3061 tttttacgcg aatataatta tctgtaaaac catacatcca caccccatct gtttcttctt
     3121 caaataatac aggacgcgtt gttgaaacaa attgatgata aaaatatcgg cgcttttttt
     3181 cgcttaaact tcttaattgc gtggtgcgtt cttcgcgtat tttttgagga acaggttcag
     3241 gtaaatttaa tgcaaaagta ttaggacgct cactataggt aaatacgtgt aaatagctta
     3301 cgggcaattc atttaaaaac tggtaagtgg tcaaaaaatc ttcatgtgtt tcacccggaa
     3361 aaccggtaat tacatctacc ccaatacaac aatgtggcat aacattttta atcatctgca
     3421 ctcgtgatgc atataaatcg cgttgatatc gtctgcgcat taatcgcaaa attttatcac
     3481 tgccgctttg taatggaata tgaaagtgtg gaacaaattt tactgacttc gcaacaaatg
     3541 caataatttc ttcttgcaat aaatttggct caattgatga aatacgaaaa cgttgaattt
     3601 tttctacttt gtccaatgcc tgaactaatt cgaaaaaaga ttcttctttt tctaatccgc
     3661 ttaaatgtcc aaaatcaccg agatttacac ctgttaaaac aatttccgca acatcgtttt
     3721 cagctatgcg atttacattt tcaataacat ttgaaatggt attacttctg cttgcacctc
     3781 gcgctaatgg aatggtgcaa aatgaacatg aataatcgca gccatcctgc acttttaaaa
     3841 atgtacgtgt gcgttcacca aacgaccagg cagtattaaa ttgttttact tcactaatgt
     3901 tgccattaat aaattgtcct ttgttattct ctgattttgg tggtaatgaa agaatatgtg
     3961 tcgtgaggtt aaatttttcg gctgcaccta aaaccaaatc tacacccgga atatttacga
     4021 tttcttcagg tttgagctga gcataacaac ccacaattac aatacgtgca ttgggtgaaa
     4081 tcttgagcgc ctggcggaca atttttttac attttttgtc tgcatgttca gttacggagc
     4141 aagtgttaac tacatataca tcggcaggct catgaaaacc ttttatctca aacccagcct
     4201 cttccatttg tctgcctagc gcagaacttt cagaataatt gagcttgcaa cctaatgtat
     4261 ataacgctac actccgtttt tgcataccct cgcaaaatta gttaattaag ccttattagc
     4321 agcctgcttc cgatgaataa accattttaa actgtcaata tccccttgtg aaggtgaaag
     4381 aaattattgc cgtttcggct gaaaattggg aaattgcacc ttatatttcg gaaaaatagc
     4441 gcgttgtatg ctcattgaac atcgtggaaa atgttttgat ctcagcaaac caatagatat
     4501 tggcatttca ttacatgatg gaacacaact caattgttat tcggcgccgc ctttctcttc
     4561 aagaccggtt gttttgggcg attttgtagg cgatatgcaa cagggtggat tgctcaatta
     4621 taaaaccgta acactcaacc cgcatggcaa tggcacgcat accgaatgcc tctcacatgt
     4681 ttatgatacg cctttaacta tcaatcaggc acttaaacaa tttcactttt tggcgcaatt
     4741 aattacgctt ataccgcata aaaccaaaga aaacgacctg gttttaaata ctgctcacat
     4801 aaaagataaa attgaacctg gtgttaatgc attgattatc agaacattac caaacgaaaa
     4861 atcgaaactt acccgcaatt ataacaagac taatccccca tatctggaag aatcgcttgc
     4921 tacctatctc aataaacagg gtattttcca tttattggtt gatttaccaa gtgtggacag
     4981 agaagaagat ggcggcaaac tggctgcaca caaggctttt tggggtattc cgaataaact
     5041 tcgcccatca gcaactatta ccgaactcat ttatgttccc aacgaagtga aggatgggat
     5101 ttacctgctt aacctgcaga ttgcagcctt tgagctggat gctacacctt caaaacctgt
     5161 gttatacccg caactctagg atgttgttga tatacaattg ccaatttgat aacattattt
     5221 taggcagcct actgcaaata ttgggtatct ttgcaccttt attttaatat atgaaaaaac
     5281 gtttacttac tactattttg ctcgcaggta tcatggtact tggacaggca caaatttcta
     5341 ttgctgacgc cagaatttta actcctggca ccaatgtaac tgtaaaagga ttggttttaa
     5401 acggtgctga attaggcgat atccgttaca ttcaagataa taccggcaat attgcagcct
     5461 ttggagctac tttgttagat ggtgttttga gaggggattc cattcaggtt accggaccac
     5521 tatttaatta taactcactt ttagaaatat cacctgttag tagtgtaacc gtaatcaatt
     5581 caggcaatcc attacctgca cctacaagta tcaccacagt ttccggatat attgaatcat
     5641 tcgaagcgaa actagtaaca gtaaatgcag ctttatttac tgaaacagga accttttcaa
     5701 ctgcgggttc aggaacaaat tacgacatta cggatgctgc aggaacagca caggttcgtg
     5761 ttgttacttc aaccaatata gatggcacac caattcctac tgatgaagtt tcagttacag
     5821 gaattatgag tcaatttgcg ccgggcggaa caggtggcta ccaattatta ccacgtgacc
     5881 ttgctgatat ttcaaccgga ggtaacccac cagtattttc aacaggttta acacaaacgg
     5941 atatcacaac atcctcattt actgtaaact tcagcacgtt gaatcctggc aatactatta
     6001 tttattatgg tctaacaacc gcattaggta gtgtttcaag tgatgctgca ttggtgacaa
     6061 ctcatgcaca caatttaacg ggattaactg caggaactat ttattatgtg caggcagcaa
     6121 gtgtaagtgc aacaaacgac acttcttttt ctgcaattag tgcaatggct acggcatcta
     6181 attctagcgg tcaaattaaa gtatggttca ataatactgt tgacaatagt gtatcaacag
     6241 gcactaatgc gatatttgta aatggtgcaa tggacgatac tattatttgg tatttagacc
     6301 atgcgaaata ttcagttgac ttagctattt ataatattga taacgtaaat aatatcatca
     6361 cagcaattaa cgatgcttat tccagaggtt tagatgttcg cgtagtttgc gatagcggtg
     6421 ttgatgattt taattatgga ttaataaata ttggtcccgg taaaaagaaa aaatcaccgc
     6481 ctgatggttc tacaaatgca gatggcgcct tgtatggtat tatgcataat aaatttattg
     6541 ttgtagatgc aatatctacc gaccctgatg atgcatgggt aattacaggc tctatgaatt
     6601 ttactgacgg ccaggttaaa ctcgataaac aaaacataat tgccatacag gatcaaagct
     6661 tagccagagg ctacaggtta gaatttgatg aaatgtttaa tggcaaattc ggtcccgata
     6721 aatcgaataa cacaccacat gaatttataa ttggcggcaa tcgcgttgaa ttatattttt
     6781 caccaagcga cgaaactgaa acgcgaatgt tggaaaaaat taacactgct gattacgact
     6841 tacattttgc tgtattttca tttacacgtt ttggcataag ttatgaaatt gaagatgccg
     6901 ttaccgaacg cggtgtattt gcagccggca tttacgacca aaccgatgca tctgactcta
     6961 ccgctgtaaa cattttagaa gacgctttgg gtgataaatt tttcaattat tccggaagca
     7021 atttattaca ccataaatat ttactaattg atgcgaattg ttcgcaatca gatccattgg
     7081 tttggacagg ttcgcacaat tggagcacta gtgcaaactc acgcaacgat gaaaatactt
     7141 tagtgattca cgattcaact accaccaaca tttattatca ggaattcatg caacgttaca
     7201 aagaagaagg tgcaacaact tttgtgagtg aaaaatgtga ttttgttggg gtggaagaac
     7261 atgttgttga aaatacaatt ttactattcc ctaacccggc tcaaaacgaa ttaaatatct
     7321 acgcagataa tcttgccgat ttcgagttgc tttatattta tgatttatcc ggtaaaaaaa
     7381 tattgtcagc agctgtaaac aatgaatcac tcattaatat aaatatcacc gatttaagtg
     7441 ctggtgttta ttttattgtt attcaaaacg gcgaaactat ttacagtaaa caatttgtta
     7501 aaaaataact tacaatttaa atcctattat gaaaagcacg ttgtatttta ttagccttat
     7561 ccttgtttct gttcaggttt tctgtcagaa ttgcgaacct gttgttgcct ataactttaa
     7621 taacaacaca actgatattt cggttaatga ttacaatgca acattattta atggcgcatc
     7681 aacagtggat gattatctcg aaattggcta caacgatgat gaccgagcag aaatacctgc
     7741 tgatgcttta aatggaattt cagactttac aataaagttc gatttttatc taacaggttt
     7801 aaacctttca ggtgcggcac ctacaaatac gataattgca ggtgctagcg caacagacga
     7861 atcagaattt gcgatttcat accaggctga tatcatggca attgtgattg cattaaaagg
     7921 aagtggggat gtttttccgg ttgaattaaa cgcggagaat tggtattgtt taacagtaac
     7981 acgagcagaa agtattgtat ctgtatttat taatgggact gaagttagca gtatagatat
     8041 gacttcgagt gatcttgata ttgaattttt agaaattggt caagagttag attgtgttag
     8101 tggttgttat gcaaccaatc aaagtttgaa cggaagaata gataacctga gtatttatcc
     8161 atgtgcaata gaaaatagca attgtgagcc ttatgttgct gaatgcgata cattagtgta
     8221 ttataaattt gatggaacag ttttagatga gggcattttt gccaacaatg gaattttaaa
     8281 tgcgggtgcc gcagtcagct ctaatattct tgaaattgat tacaacgata acgactatgt
     8341 ggaaattccg tcaagtgctg cagatggttt agatgaattt gccatttcct ttaattttta
     8401 tcttaacaat ttaaatgaag acggctcatc tccaactaat acatttattg cgggaacgga
     8461 tggaatagat atgcatgaac tagctttgtc atacgagtca agttcaaacg cttttgaagt
     8521 agctattcat gaaattggcg gtataatccc tgcaacaata ttacctggca cttggtattg
     8581 tgttacattt tatcgcaaaa gcgatagtgt ttggattgag ttagatggta taatattacc
     8641 aactgttttg tcattacccg aaggtccttt atcaatttca tttcttgaaa ttggtcagga
     8701 gttggattgc gcagaaggtt gttttgctga aaaccaatcc ttaaatggta aaatggataa
     8761 tttaattatt caagggtgtt caactataat caattgcaat ccggcaagtg taggcattta
     8821 ttcaaatcaa aattcagatg ttgctataac cccaaatccg gccagtaatt ggattactat
     8881 aaatggggtg aatttaaatt cagcaaatat ttcaattacc aacctgcaag gtcaaaagct
     8941 acctcttgag attctcaata atacattagt aaatatcgct catttacctg aaggaatgta
     9001 ttttttgatt attgaaagta atggatccgt tatggtcaag cgttttataa aagagtaaaa
     9061 ctcaacttag gtaattcaca atttcaaatt taagatcttg tagaatcata atgtaggcat
     9121 aattatttat tccaataact atataccttc ataaactttt tcgaaagctg ctgcaatatt
     9181 ttctgcatta aaaattgtta tgttttcagc tgctattttg gcgttatcga atttagtttc
     9241 aattaacttt tcaagcgcat tattccagcc ttctattgta ttgggtgtag taaatccata
     9301 accttgtttt accaattccg gaagtgccgc tacatcagaa actaaaaccg gcgttccagt
     9361 aactaatgcc tctgctgtca ccaccgaaaa cgtttcaaac ttagaactga ataaaaaata
     9421 atgcgaatcg ttaagctgat tggcaatttc ctgtcgggat tgaaacccac taaataaaat
     9481 tggataactc catttcaagg atttaatttc gtcgtacaaa atacctcctc ctattaatac
     9541 aagttctatt gattttcccg atttacgcac taattgttct aatgaattta caattaaaaa
     9601 caaatcccta tcggcattcc aattagaagc cactgcaata aatctgatag gcatttcagg
     9661 tttttgaatt tttggtgtat aatgaaataa attaccatct acaacgttcg gcacaattac
     9721 actttttcca acaagtgctg gtataacctt acaaacttcg tgttgaagcc acttactgac
     9781 aaatgttact ttttctgcat atttaaaggc ttttatacct ataaaggaaa gcaaatgtga
     9841 agtgagaaat tttgttgttt tagaccagtg ttcagtcaat actaatggtt tatttatttt
     9901 ttttgcaatc agataagcaa gaactcccgc agggaataca accataccat gaaccacatc
     9961 aatttgatca aaattttccg ttttaatcct tttttttatt gatggccaaa gtaatattgg
    10021 gaattggtac aaaagtttat ggaacttaga tgttacaaaa atttcgacaa tttttaagcc
    10081 ttcataatca tacttgctaa tagattgggc atataactta tgtgattgtt caactcttat
    10141 atataacacg aaggctaaat gttgtgtttt acttatagcc tttaaatgct caactataaa
    10201 ggggaagcta agaggttttt ccttagttgg aaaccaaaat gtaacggcag caattttttt
    10261 taccatctca cactcaacaa aaaattatat tctatctttg caataataag cgaaaatgtc
    10321 gaataatcaa acacctaatt ttgaccctca agcatattgg gaaaaacgcc taaaaagcaa
    10381 cactggttta acaggagtcg gatatacgct ggttggcgaa cactacaata aatgggccta
    10441 caaaattcgg aagtacgttt ttaataaaat agttacgaaa aacctaaagg accagaaaaa
    10501 tgctgacatt ttagatatcg gctcagggag tggtttctat attgatgaat ggctgacata
    10561 tcaacctaaa tcaatgacag gtattgacat tactgaaatt gccgtatccc gtttgtcgga
    10621 aaaatacacg gatctaacat ttcataaatt agatatcagc gatttagctg ctacccaact
    10681 tatgtttcaa caaaaatttc atgcaatctc ttgtatggat gtattgtttc atattgttga
    10741 tgatgaaaaa tataaatcaa catttcaatc tgtcgctcag ttgctggtta atggtggcta
    10801 ttttattttt tcagaaaatt ttgtgcatga acaattatca actttaaaac atcacaaatc
    10861 aaggtctaaa gtagaaatcg aacaattatt agctcagaat aattttaaaa taatcagccg
    10921 acgaccattt atgtatttaa tgaatgaacc tgttgattct aatagtttta tacttaaatg
    10981 gctctggaaa atcactgcaa tcatttgcgc caaatcagcg cttttgggat atattatagg
    11041 agcaataacc tttccgattg aaaaattgct agtatccctg ttaaaagaaa gtcctacaag
    11101 tgaaatagtt attgcagtta aattaaaata atagttttat cacttttgta actagttctc
    11161 aagccaacaa tatcggttta ttatctattt atgataaata aaaaagagga gctgttttaa
    11221 aacaactcct cttttcatat ataatgctta aaatttattc cttcactata gaacacgatt
    11281 ttgtcgcgct agtgttttca tttacaactg ttacaaaata cataccgggt gcgaaattag
    11341 ctatagaaaa tccatgctgt aattgttcta cattgcaggt agctaccgac ttacctgcaa
    11401 tatcagtaat gagcacctta tcatctaaat tatatccttg aatttcattc atataaatga
    11461 aaccattact aggattagga taaataaacg agtccgaatt agatgaagtt aaaactaatt
    11521 tgccgtcatt tggtgaacac actgtactca tatttaaagc aagaggttta atacctggaa
    11581 atgagcttgg gtaggataat gttactgttt cagattctag tgtttcaaaa ttatcataat
    11641 tagtaacaac agttgaagta tttaatattt cggtttcaca cacaccaatt tctccagtta
    11701 aaattgatgt tttcatgaat atcccttcaa cagttcctcc atcaccaact tgttcaccaa
    11761 ctacaaaaat atcatctcct actattgtca ttgcgcttga actaaataac ccggtggtag
    11821 ggtaccttaa tctaaaagga taagatttag cccaaacaac attaccatcg aaatccaggt
    11881 tatataaaca aacattagta tactttccat cttgatataa actaccataa acaatatatc
    11941 ctgattcgtg ctttttaatt ccggcaaata cgccatcata aaatgcgcct gttacatcaa
    12001 ttttacgagt tataactgcc tcaccggaca aatctgtttt gataaaccaa aaatatttat
    12061 ttgagcttgt gcttacatca tcaccactac ctatcacaat aatatgttcc ccatcaataa
    12121 gcatatcttc agaatagaac cttcctgagg ttgctgtact tttaatataa gacctggtcc
    12181 aatcaattgt tcctgttata tcaaatttat taatacaaat cctaaattta ttatttcctc
    12241 cagttgaaag ctcgtaacgt ccggttgaat atatggaaga tgaagttgga tcataagcaa
    12301 ctgcctctac agattcattc atgtttttat tcaaattcgt aattactgac tgagcaccgg
    12361 aagctcgttt aaacttaatc gtaatatcat cagatccggc acctgttcca ttatttgttt
    12421 cttgcccacc taccagatag tcacctcctg gaccaagttc cactacgtcc caaaaaaagc
    12481 tgttaacagt gcactttcta aaccatacaa tatcctgaat tactgggtca aatttaacta
    12541 caaatgattt ccctaaatca cccgcaatag aagtcccaac caatacaaga ttaccatcac
    12601 tatctttaat catttggttt attgtcatgc tagtatcctt agcattaata cgtttattcc
    12661 aaacaagcac tccgctatga tctatttcac caataattaa ataaccttca tttgatcccg
    12721 caaggtaaag atttgaagtg gttgtttcgt caacaggaac tagcgcagca aaacgtattc
    12781 cctgttcagg accattgtca taagatttta tgaattgcgc gtcaactatt tttaccgtta
    12841 gcaacagtga aaaagcaatt aataatttta aaattttcat agttatattt tttgaaattt
    12901 agttattgaa ctaccggata tattattttc aatgataata tataatccag gttcaaattg
    12961 tgaaatgttt acatgtaaat aatcgttatt acaaatattg gaaaaaacta atcggccatt
    13021 gcaatcataa atttgtaacg acgaaccttt ttgcaaatta gtaattgaaa tcaattcagt
    13081 agcaggaacg ggataaactg aaatttgatc gtccttaatt aagtcttgaa ctgtattatt
    13141 actcctccca ttgggatttg gtatgtttaa aataatttcg gttctcccgg caaaattttt
    13201 cttagttaat ttttttcctg caatattgag catagcaggt tgatttatcc atgtgtcaat
    13261 tgagccatcc aaacgcttaa acaatttgga attttcctca ttactaacag tagaaactcc
    13321 gttactatag gcatatgtat aagccatagt gtaagtcgca tctaccgcat tatcaacagg
    13381 aaaaacgccg taatacatat ccggagaatt tatcagacca ttgtccccaa taggataact
    13441 atccacacga tataaatgta ttccatatgg agtgcctaaa attttattta cttttaattt
    13501 atcgccaatc attgaattaa gcgataaagt tgctcccgaa taatctgtct gataaacaaa
    13561 aacactggca ttacctatag gtgcacctga attagatttc tctacactac caatttcgtt
    13621 gccatttgct ccggtaactg aataatcttg aagcgtagtt tcagttgtat tttcatcaac
    13681 aatccaatat ccgatcagcc cggtctctga taatgtcagt ttttcgcaca ttttatctcg
    13741 tatatcaagt tctgtccgag caacattcca taaccttacc tcatcaattt taccaataaa
    13801 tgcatccgat ctatgcaccg tagcatatct acctaggtta actttattac tgttatgcac
    13861 aattgatgtg tttgatgcag ttccatcagt tgatacagca gattgcaaaa caccattaaa
    13921 ataaaactta atgtcagtaa ccgaggtagc aacaattgcg atgtgcaccc attcatttaa
    13981 aggcactgtg cttgttgtta tttttcctct tcgatgcgca ccacctgcac ccacgccatt
    14041 acctatttca aaaattaatt tcccaaccgg atcaaatcgg atataaaacc catagtaatt
    14101 acctgatgta taactgtctg atgcaaatac tggtgtgtat aacgaatatt ctgattggtt
    14161 aagccacgct tcaaaagtca aagggaagtt tagtgtatta taattatcac ctaaatcgta
    14221 ataattatcc gaattcccgt caaaattcaa tgccaaaccc gagccaacaa agttggactg
    14281 gctatttcct atttgaacca tcgatcctaa taaggcgatg ataataacta tttttaattt
    14341 cattgtttta caattttaga tgtaatattc ttgccattta cttgttgtga tacaaagtaa
    14401 ttcccagcag gcaacctact taagtcaaga gttatcaatt ctccgacttc aaaaacagta
    14461 taatgcctaa ttattttgcc ttggttatca cttacagtaa ttggattagt ggacagtgat
    14521 tttgttataa agtaatctgc actcggattt gggaatacct ccaattccgg agtgatttca
    14581 gctttcatct cattaacaaa tggaatatta agtatgtgtt cacctcttcc aatacaattc
    14641 tttttaacca aactggaatt ggg
//
LOCUS       DAORKW010000029        14529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_53_length_14529_cov_5.747340, whole genome shotgun sequence.
ACCESSION   DAORKW010000029 DAORKW010000000
VERSION     DAORKW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14529
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53_length_14529_cov_5.747340"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PLB46_02450"
     CDS             complement(<1..912)
                     /locus_tag="PLB46_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRF75403.1"
                     /translation="MKLTTTPKRLTAYSTMAAAVLAIPNDSDAQVIYTDINPDKVVFT
                     TGPLDSLHLDVDGDSVTDFIFNAFYFYSSYTASGNNYDHLGVQGVNGARIAYTLQSTN
                     IYTFSNALFTNLSAKFAQILPANTPINNTLNFANNAEFFYQQCFNAIYSSAPDYCWTQ
                     GMGIGVVKYIGFRLKNGPLKYYGWMRLSISNTTPFVPVPTSINILDYAINWTPNTPIL
                     AGQGLICIVTDPLGTGTTTAHWAKVYWEPQPGVSYYEVAYREPGGFWETKLVAPDKTN
                     TKLNGLTCSTNYEWQIRSACADGSMSDY"
     gene            complement(1031..1912)
                     /locus_tag="PLB46_02455"
     CDS             complement(1031..1912)
                     /locus_tag="PLB46_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRF75404.1"
                     /translation="MQQQYNAKNDNIFVWVKDKLYPRNEAKVSVFDSSVQGGDAVWEG
                     LRVYNGKVFCLSEHLERLQASAHAMMFANVPDNDFITTAIYATLKANGMSDDAHIRLT
                     LTRGEKITSGMDPRLNQLGCTLIVLAEWKAPVYPNAITLITSSIRRNGPANIDSKIHH
                     NNLINNILAKIEANNAGADDALMLDNNGFVAETNACNIFMIKNGMVFTPNADACLPGI
                     TRKTVISLCQQNNIPIHLKNISLTEMYNADQVFTTGTMGELTAVKMIDGRAIINRSGA
                     GLMQHIRNLFRNLTQAL"
     gene            complement(1914..2636)
                     /locus_tag="PLB46_02460"
     CDS             complement(1914..2636)
                     /locus_tag="PLB46_02460"
                     /inference="COORDINATES: protein motif:HMM:NF040370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75405.1"
                     /translation="MKPAQIIINLWSGPRNISTAMMYAFSQRKDTRVFDEPLYAHYLR
                     VTGLQHPGREEVMAAQNANGDEVMDGFLFDNFTRDVLFLKQMTHHLVDMDLRFLEQTK
                     NIILIRDPKKVLHSYGKVIAEPTLEDIGIKQSFELFEYLQKLHLHCLVVDADTILKNP
                     ETALQAICKSCGIPFLPAMLSWAPGPKKADGVWAKYWYQNVHTSSGFQEYVEEEIHLL
                     SNLQKVYAEALPYYEKLKQHAI"
     gene            complement(2670..4961)
                     /locus_tag="PLB46_02465"
     CDS             complement(2670..4961)
                     /locus_tag="PLB46_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRF75406.1"
                     /translation="MKLLDTLNEAQLAAVTNIHGPTMIIAGPGSGKTRVLTFRVAYLM
                     YSGIDPFSILALTFTNKAAREMKNRIEQLYGTDARNLWMGTFHSVFSRILRSEADKLG
                     YPSNFTIYDSEDAKNLLKTIVKEMGLNDKLYKPGYVLYRISLCKNNLIGPEAYAVNTD
                     LISEDEGNGRPKMAEVYKNYTKRCFRAGAMDFDDLLLKTHELLERFPDVLYKYQHRFK
                     YVMIDEFQDTNFLQYSIVKRIADVFQNIAVVGDDAQSIYAFRGASIANILNFEKDFPE
                     VKTFRLEQNYRSTKIIVEAANHIIGKNQHQLAKKIWTDNDEGDTIKVIRAMSDNEEGR
                     MVADSIFEQRMRDHVKNGEFAILYRTNAQSRAFEEGLRKLNIPYRIYGGISFYQRKEV
                     KDLIAYLKLTVNPHDEESFKRVINYPARGIGKTSIEKLMVYASDNDKSLWTMSENIDL
                     LQFNSGTRKLFSDFVTMIKSFQTMLDKKDAYEVASHIAKSTGLLKELYNDQSVEGLSR
                     YENIQELLNSIKEFVAPDEADSPEFAPTFTDELKEEKTLTNYLQQVVLLTDADAEGNE
                     DETVKLMTIHAAKGLEFKSVFVVGLEENLFPGTLSVSSREDLEEERRLFYVAVTRAEK
                     KLTITHAATRYKYGNLEYCEPSRFLEELPQNLLQYVGYNSKSWTEEKSNKQYENGTPV
                     TKVKPAVPVNNFVHKPSENFVAAFPEELQSGMDVEHQKFGFGKILAMEGANDGKMATI
                     FFQNAGQKKLLLKFAKLSIVKSA"
     gene            complement(5020..5736)
                     /locus_tag="PLB46_02470"
     CDS             complement(5020..5736)
                     /locus_tag="PLB46_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75407.1"
                     /translation="MATKKFVQALSLKSKNYRKVAAIEIVLIFLVLILGDMMIKLDVG
                     NPHTLVSAILLIPEAVYLIILYQLGQLLNPDKKVQQIIKIAIIIVFLISFVALNPFVD
                     VTEWKTNLLILTHVFLCGIECYIIALGLKDIYAENLHIAERLWGSVAVYLLIALGWAS
                     FYEVFLLLDHSALGFALEPGYQTYSESLYFSLCSLSGTGSAYPNPTHIIRNLALIESV
                     WGVLFLVMLIGRLFSLPAKD"
     gene            6081..8234
                     /gene="recQ"
                     /locus_tag="PLB46_02475"
     CDS             6081..8234
                     /gene="recQ"
                     /locus_tag="PLB46_02475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRF75408.1"
                     /translation="MTPELILKKYYGYNSFRDKQADIIQHVLNKQDAVVVMPTGGGKS
                     VCFQIPAMLLPGITIVVSPLIALMKDQVDGLNAIGIPAIALNSSMNAAEQQEAYKRIN
                     NGEIKLLYVAPERIMSEGGRFIEFLKSLPISLFAIDEAHCVSMWGHDFRPEYLQLAVF
                     KQQFPGIPVIALTASADAITRKDIAEKLLIAKSKIFISSFNRANINYIIERKKDALDA
                     IIEYIDAHKNKSGIIYTLSRNGAETLAAQLSDAGFAAKYYHAGMDSASRAKVQEEFKT
                     DRINIIVATIAFGMGIDKPDVRFVIHYNMPKNIESYYQETGRAGRDGLKSDAILFYSY
                     GDVMALKRFVEVEDKTQAAIMYKKLDQMAGFCDARKCRRKMILEYFGETYSVDNCQSC
                     DYCLSKRNSFEATVIAQKALSAVVRTEERFGVNYLIDFLRGSKSEKIWDKHKLYKTYG
                     IGADISKEKWTLYFRELIDQGFLHRDESGDYPRLTLTEKSQLVLQGRQEVFFSLVEEK
                     ARAKAVLEIEYDTALYEQLRSFRTKIAYNENLPPYMVIGDATLQSMAAYLPQSLSDLP
                     FIPGFGEIKIERYGKKLLEIVVSHCRLNKLTSRMELINKRAAKKSSGSKPAKIAETKK
                     TTLYMFRSGKSIEEIAAERNYAISTIEGHLCEFILTGDVSVNQFVSPEKIEVITRYIK
                     HSTDKKITPLKEKLGDDYSYFEIRAVLSHLDYLLT"
     gene            complement(8236..10326)
                     /gene="scpA"
                     /locus_tag="PLB46_02480"
     CDS             complement(8236..10326)
                     /gene="scpA"
                     /locus_tag="PLB46_02480"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRF75409.1"
                     /translation="MNRNSYNKMKRDFKHIPYQHFSINHDFVAPTDDFKNVIDTQPGL
                     APFLRGPYTSMYVMQPWTIRQYAGFSTAEASNAFYRKNLAAGQKGLSVAFDLATHRGY
                     DSDNERVVGDVGMAGVAIDTVEDMKILFDQIPLDQMSVSMTMNGAVIPIMAFYIIAAE
                     EQGVQPHQLAGTIQNDILKEFMVRNTYIYPPAPSMRIVADIFKYCSSNMPKFNSISIS
                     GYHMQEAGATPEIELAYTLADGIEYVRTGINSGLKVDDFAPRLSFFFGIGMDHFMEIA
                     KLRAARFLWYKLMQQFAPQNAKSLMLRTHCQTSGWSLTEQQPFNNVARTCIEALAAAL
                     GGTQSLHTNSLDEAIALPTDFSARIARNTQIYLQQETHICKTVDPWGGSNIVEALTEK
                     IIEDAWQLIEEVEQLGGMQKAIETGIPKLRIEEAAAKKQGKIDGGSEIIIGLNAFITD
                     EQSNIDILEVDNSAVRLSQIERINQVKSQRDNAAVENILQQITNCAATGEGNLLALAV
                     DAARKRATLGEISFAMEKVFGRYAANIQTVAGVYQKEVMDNESFINARQLSDQFAEKE
                     GRRPRILIAKLGQDGHDRGAKVIASAFADIGFDVDISPLFLTPAEAVKQAIENDVHVI
                     GISSLAAGHKTLVPQVIDLLKEQGRGDILVVVGGVIPQQDYNFLHEAGAAFIFGPGTV
                     ISDAAGELLTKMLN"
     gene            complement(10335..11540)
                     /locus_tag="PLB46_02485"
     CDS             complement(10335..11540)
                     /locus_tag="PLB46_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRF75410.1"
                     /translation="MKDLFHDVSPIPEEKWFEQVTKDLKGADFNEKLVNTIDQITIQP
                     LYTRKSLPQFTVDADAEFMLDLDPEFVEAISDQGQQSLTWNITEDVLVTENDNLNNLI
                     AAARARGASHVRISVTNDEVWDHVVNEIIKLTTPANFIFNVYGDLSEGDVVNKWRDRV
                     GHLGERDRLLFGIEFDPIGYWQQYGNDTYNGTMFAHLADLFFRLSGHLHDCKLIKIDT
                     THFANQGEDVVSQLVKTLQITSTYFDEMEKRNVPLEELVQLCTFRFGIGSNFFFEIAK
                     LRAFKILWHNLIKVYLPEMDFITNPEIHCVTATASFTQTDEHSNLLRTTTAAMSAILG
                     GCDVLIVTPFSNSGENNAIQLATNIQNILRYESYIDRYRDAANGSFYIESLTQQFGQQ
                     AWQQFIDSK"
     gene            11602..12390
                     /locus_tag="PLB46_02490"
     CDS             11602..12390
                     /locus_tag="PLB46_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRF75411.1"
                     /translation="MQLENLTTEIKGNILYITINRPKNLNALNIKTIVELDEVFEEYM
                     YNDDVKGVILTGAGEKSFVAGADISEFADFTPAQGKTMSENGHDTFDKIENYTKPVIA
                     AVNGYALGGGCELAMACHLRVASENAIFGQPEVNLGIIPGYGGTQRLVELIGKGKALE
                     LLMTGDSINAAMAKELGLVNHVVAQADLMAKCEEILNKIMSKGPVAVAKVIQCVNAHY
                     RGGGYVGDSYQGEITQFGQCMETEDFVEGTSAFLEKRKPEFKGK"
     gene            complement(12611..14242)
                     /locus_tag="PLB46_02495"
     CDS             complement(12611..14242)
                     /locus_tag="PLB46_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75412.1"
                     /translation="MKPTILSAILWLGIMGTSFGQSFHFLENIDNGMPTNTVNIATDP
                     TGNTYMTGTFFYELTIQDITLTSDEYMTCFLAKYDKNGILKWLKPIPASGANFPTGIV
                     FNKVTKGVTLCGGFNDTMNINGVTHISNGNIDAFWMSLDANGNILNGATAGGDSSDIF
                     YDVEIDKGGNVYVLGNSDSQEMQFNGVNQFMDATENTGLMAKFTADGSPVWMRTMTGD
                     ANFLAYEMAVGNNGTAYLGFKSTGSYFIHNGPDELYGLIKDISETNIILFSFTKNGDV
                     FQSTEFETSSIYSSVSDVIFDGSATIAINFVDSIQVFGEYYYAEDGTGSIIARINFNT
                     LEPEWAKFIFSPYGTGIMSLKNNGNTILCGGDFIYYAECDGLTVENPFADPDALLVTL
                     NRNTGLVLGFADFDNVESYDYIVDVDVDKNKNIYLAGMFNGLIYFDAMSATSNDFYYN
                     IFLTRLNATIFKEEAISDQSAITLYPNPAVEYITINGEIQLWEIINMEGQIILSGIGS
                     TSSTTINLQSIPSGFYLLSTTNTNNEIITQEFVKQ"
BASE COUNT         4483 a         2694 c         2780 g         4572 t
ORIGIN      
        1 ataatcagac atagagccgt cggcacaggc gcttcgaatt tgccattcat aattggtgct
       61 acatgttaat ccatttaatt tagtatttgt tttatcgggc gcaaccaatt ttgtttccca
      121 aaatccaccc ggttcacggt aagccacttc ataataggat actcccggct gtggttccca
      181 atatacctta gcccaatgcg ctgtggttgt gccagttcca agcgggtcgg taacaataca
      241 aatgaggccc tgacctgcaa gaattggtgt attaggtgtc caattgatgg cataatctaa
      301 aatattaatg gaagttggca cagggacaaa cggtgtagta ttgctaattg aaagtcgcat
      361 ccaaccataa tattttagtg ggccgttttt taaccggaag ccaatatatt ttacaacgcc
      421 gatacccatt ccttgagtcc agcaataatc aggcgcactc gaatatattg cattgaaaca
      481 ttgttggtaa aaaaattcag cattattagc aaaatttaat gtgttattga tgggtgtatt
      541 tgcaggtaat atttgtgcaa attttgcact tagattagta aataatgcat tcgaaaaggt
      601 ataaatattt gtcgattgca aggtataggc aattcttgca ccattaactc cctggacacc
      661 aagatggtcg taattatttc ccgaagccgt ataggaagag taaaaataaa atgcattaaa
      721 aataaaatct gttactgagt cgccatctac atctaggtgt aaagagtcca aaggtcctgt
      781 tgtaaatacc accttatcgg gattaatgtc agtgtaaata acctgggcat cggaatcatt
      841 tgggatggct aaaactgcag cagccatcgt agaataggct gtcaagcgtt tcggggtagt
      901 tgttaatttc atatggaagg ttttatcaaa tataacaaaa tttgacacca tatgcaatcc
      961 tctatatatt taattaaatt acaattgggc aattaccaaa tgattacaga ctactccacc
     1021 tattcaggta ttacaaagcc tgtgttaaat tgcggaacaa attgcggata tgttgcatca
     1081 aaccggctcc actcctatta ataatcgctc tgccatcaat cattttaaca gcggtaagtt
     1141 cacccatcgt tcccgttgta aatacctggt cggcattata catttcggtg agtgaaatat
     1201 ttttcaggtg tatgggaata ttattttgtt gacataaact gatgacagtt tttctggtaa
     1261 ttcccggtaa acacgcatca gcatttggtg taaataccat gccgtttttt atcataaaaa
     1321 tattacacgc gtttgtttct gcaacaaaac cattattgtc taacatcaat gcatcatcag
     1381 ctccggcatt attggcttca atttttgcga ggatgttatt aattaaatta ttgtgatgaa
     1441 ttttagaatc gatattagca ggaccattgc ggcgaatgga agatgtaatt aaggtaatag
     1501 cattaggata taccggagcc ttccattctg ccaaaacaat taatgtacat cccaattgat
     1561 ttaatcgcgg gtccattccg gaagtaattt tctcacctcg tgttaaggtt aaacgaatat
     1621 gtgcgtcatc agacatacca ttggctttta atgtagcata aatagccgtg gtaataaaat
     1681 cattatccgg cacattcgca aacatcatgg catgtgcact tgcctgcaaa cgttcaaggt
     1741 gctcagataa acaaaacacc tttccattgt aaacacgcaa tccttcccaa acagcatcac
     1801 caccttgcac agatgaatca aatacagaaa cttttgcttc attgcgtgga tataatttat
     1861 cttttaccca aacaaaaata ttatcgtttt tggcattata ctgctgttgc atattaaatg
     1921 gcgtgttgtt ttaatttttc ataatagggt aaggcttcgg catatacttt ttgtaaattt
     1981 gacaataagt gtatttcctc ctccacatat tcctgaaatc ctgatgaggt atgtacattt
     2041 tgataccagt atttcgccca aacaccatct gcttttttgg ggccaggcgc ccagcttaac
     2101 atcgcaggaa gaaatggaat accacacgat ttgcaaattg cctgtaatgc cgtttcaggg
     2161 tttttcaata tagtatccgc atccactaca agacaatgca aatgtaattt ttgcaaatat
     2221 tcaaacaact caaaactttg tttaataccg atatcttcca aggtaggttc agcaataact
     2281 ttgccgtagg agtgtaatac ttttttaggg tcgcgaatta aaataatgtt ttttgtctgc
     2341 tccaaaaatc gcaaatccat atctaccaaa tgatgggtca tttgttttaa gaataacaca
     2401 tcacgagtaa aattatcaaa taaaaaaccg tccatcacct catcaccatt ggcattttgt
     2461 gctgccatta cttcctcgcg tccggggtgt tgcaaacctg tcacacgcag gtagtgggca
     2521 tataaaggct cgtcaaatac gcgagtgtct tttctttgtg aaaaggcata catcatagcc
     2581 gtagaaatat ttctcgggcc agaccataaa ttaataatta tttgagccgg tttcaattgt
     2641 tacaattatt ttcaggttaa acaaataact taagcagatt taacaatact cagtttagca
     2701 aatttgagta ataatttttt ctgtccagca ttttgaaaaa agatagtagc cattttacca
     2761 tcgttagcac cttccattgc caatattttt ccaaaaccaa atttctgatg ttctacatcc
     2821 atgcctgact gcaattcttc agggaaagca gccacaaaat tttcggatgg tttgtggacg
     2881 aaattattaa caggaacggc aggttttact ttagtaacag gtgtgccgtt ttcatattgt
     2941 ttattacttt tttcttccgt ccagcttttt gaattataac ctacatactg caataaattt
     3001 tgtggcaatt cttccaaaaa acggcttggc tcacaatatt ctaaattccc gtatttatat
     3061 cgcgttgcgg catgcgtaat ggtcagcttt ttttctgcgc gggttacggc tacataaaac
     3121 aatctgcgtt cttcttccaa atcttcacgg gaagaaacag atagtgttcc cgggaataaa
     3181 ttttcttcta acccaacaac aaacacactt ttaaattcca gtccctttgc tgcgtggatg
     3241 gtcattaatt taaccgtttc atcttcattg ccttctgcgt cagcatcggt taataaaact
     3301 acctgttgca gataattggt caatgttttt tcttctttca gttcatccgt aaaggtcgga
     3361 gcaaattccg gagaatcggc ttcgtcggga gccacaaatt cttttattga gtttaataat
     3421 tcctgaatat tttcatatcg gctcaaacct tcaacacttt ggtcgttata taattctttt
     3481 aataaacccg ttgatttggc aatatgacta gcaacttcgt aagcatcctt tttatcgagc
     3541 atggtttgga agcttttaat catggtaaca aagtcggaga acaattttct ggtgccggaa
     3601 ttaaattgca ataaatcgat attttccgac atggtccata aacttttgtc gttgtcggaa
     3661 gcgtatacca ttaatttttc tatacttgtt ttcccaattc ctcgggctgg ataattaatt
     3721 acacgtttaa acgattcttc atcatgcgga ttaacggtca gttttaaata cgcaattaaa
     3781 tcttttacct ctttgcgctg ataaaacgaa ataccaccgt aaatgcgata aggaatattg
     3841 agtttgcgta aaccttcttc aaatgcacgg ctttgcgcat tagtgcgata tagaattgcg
     3901 aattctccat ttttaacatg gtcgcgcata cgctgctcaa aaatgctatc cgccaccatt
     3961 cttccttcct cattatcgct cattgcgcga ataaccttta tagtatcgcc ttcatcgtta
     4021 tcggtccata tttttttagc taactgatgt tgatttttgc caataatatg attcgccgct
     4081 tcaacaataa tttttgtaga acgataattt tgttcgaggc ggaaagtttt tacttccgga
     4141 aaatcttttt cgaaatttaa aatatttgca atgcttgctc cgcggaaggc ataaatgctt
     4201 tgtgcatcat caccaactac ggcaatattt tgaaatacat ctgcaatacg ttttacgatg
     4261 gaatattgta gaaagttggt atcctgaaac tcatctatca ttacatattt aaaacgatgc
     4321 tggtatttat ataaaacatc aggaaaacgt tcgaggagtt catgtgtttt taataataaa
     4381 tcgtcaaagt ccatcgcccc ggcacggaaa cagcgttttg tataattttt atacacttct
     4441 gccattttag gtcggccatt tccttcatcc tcactaatta agtcggtatt aaccgcataa
     4501 gcttcaggac caattaaatt atttttacaa agtgatatcc tgtataatac atatccgggt
     4561 ttatacaatt tatcgttcaa tcccatttct ttcacaatgg ttttgagtaa attttttgca
     4621 tcttccgagt cgtaaatggt aaagttggaa ggatatccca atttatcggc ttcgctgcgt
     4681 aaaattctgg aaaacacgga gtggaaagtg cccatccaca aattgcgcgc atcggttccg
     4741 taaagctgtt cgatacgatt tttcatttcg cgggcagctt tgttggtgaa agtcagcgct
     4801 aatatcgaaa acgggtctat gcccgaatac attaaatacg ccacacgaaa agtaagcact
     4861 ctggtttttc cagaacccgg tcccgcaata atcatagttg ggccgtggat attggtaaca
     4921 gccgccaact gagcttcgtt taaagtatct aataatttca cgggttaaaa atacggcaaa
     4981 gagtcaggct tatacaagta tttggtgtgg atatagcttt taatctttgg caggcagtga
     5041 aaacaatcgg ccgattaaca tcaccaaaaa taaaactccc caaaccgact caattaaggc
     5101 caggtttcga attatgtgcg taggatttgg ataagccgaa cccgttccgc ttagactgca
     5161 gagactgaaa tataaacttt cggaataggt ttggtaaccc ggttcgaggg caaaacccaa
     5221 tgcgctatga tcgagtaaca aaaaaacttc gtaaaaactt gcccatccca aagcgattaa
     5281 taaatacact gccaccgaac cccataaacg ttcggcaata tgtaaatttt ctgcataaat
     5341 atcttttaat cccaatgcaa taatataaca ttcaatcccg cataaaaata catgggtgag
     5401 gattaataaa tttgttttcc attccgttac atcaacaaaa ggatttaggg cgacaaaaga
     5461 aatcagaaat acgataataa ttgcaatttt gataatttgc tgcacctttt tatcagggtt
     5521 taacaactgt cctaattgat acaggataat caaataaaca gcttcaggaa ttaacaaaat
     5581 tgcggatacg agggtatgtg gatttcctac atccagttta atcatcatat ctcccaatat
     5641 caatactaaa aatatgagta caatttctat tgccgctact ttgcgataat ttttggattt
     5701 gagactaagc gcctgaacaa attttttagt tgccataaaa agctgtgctt acctgagcat
     5761 cgaagataat aaaaacagcg aaagtttatt taataattat acatttttgg ttcaaaagat
     5821 ggcctgtatt gagttgtggt tgattgccaa ttgtaatatt tcattaaatt acagttcaaa
     5881 aaggggttgg ctatcgtttg cagttaattg ttttttaact gataatttta ggttttagaa
     5941 aaacaaagcg atgttgctga aaaaaaattt atttcatttt tccgctaaaa ttaaagcaat
     6001 aaatggctca aagcgagtaa gttcgttttt tgtttggtga taccgaagca atagttttat
     6061 ttttgatgga aatatctctt atgacacccg agcttatatt aaaaaaatat tatggttata
     6121 atagcttccg cgataagcag gcagacatta ttcagcatgt attaaataaa caggatgctg
     6181 ttgtagtaat gccaaccggc ggaggaaagt cggtttgttt tcagatccct gccatgttac
     6241 ttcccggaat tacgatagtt gtatcaccgc ttattgcatt aatgaaagac caggtggatg
     6301 gtttaaatgc tatcggtatt ccggccattg ctttaaatag tagcatgaat gcagctgaac
     6361 aacaagaagc atataaacgt attaataatg gtgaaataaa attattatat gttgcacctg
     6421 aacgtattat gagtgaaggt ggtcggttta ttgaattttt aaagtcgctg ccaattagtt
     6481 tgtttgccat tgatgaagct cattgtgtaa gtatgtgggg gcatgatttc agaccggaat
     6541 atttgcaatt ggcggttttt aaacaacagt ttccgggtat tccggttatt gcattaacag
     6601 ccagtgctga tgctattacc agaaaagata ttgcagagaa attattaatc gccaaatcga
     6661 aaatttttat ttccagtttt aatcgtgcga atattaatta catcattgaa cggaaaaaag
     6721 atgctttaga tgcgattata gaatatattg atgcgcataa aaataaatcc ggcatcattt
     6781 atacattaag tcgcaatggc gctgaaacat tagcagccca actcagcgat gccggatttg
     6841 cggctaaata ttatcacgcc ggaatggata gcgcctctcg tgcaaaagtg caggaagaat
     6901 ttaaaaccga caggattaat attattgttg caaccatagc atttggtatg ggtatagaca
     6961 aacctgatgt gcggtttgtt attcactaca acatgccaaa aaatattgag agttattatc
     7021 aggaaaccgg cagagcaggt agagatggct taaaaagtga tgccatatta ttttattcgt
     7081 atggcgatgt aatggcgctt aaacgatttg tggaagtaga agataaaaca caggctgcta
     7141 ttatgtataa aaaactggat caaatggccg ggttttgtga tgcgaggaaa tgcagaagaa
     7201 aaatgatttt ggaatatttt ggcgaaactt attcagtgga caactgtcag agttgtgatt
     7261 attgtttaag caaacgaaat agtttcgaag cgacagttat tgcacaaaaa gcattaagtg
     7321 ctgtagtgcg gactgaagaa cgatttggtg ttaattatct catcgatttt ttacggggaa
     7381 gtaaaagtga aaaaatatgg gataaacata agttatataa aacctatggc attggcgccg
     7441 acatatcgaa agaaaaatgg actttatatt tcagggaatt aattgaccag ggatttttac
     7501 atcgcgatga atccggcgat tacccaaggc tgaccttaac agaaaaaagt cagttggttt
     7561 tacaaggcag acaagaagta tttttttcgc ttgttgaaga aaaagcgcgc gcaaaagcag
     7621 ttcttgaaat tgaatatgat acggctttat atgaacaatt gcgcagtttc agaacaaaaa
     7681 ttgcgtataa cgaaaacctc ccaccttata tggtaattgg tgatgcaact ttgcaaagta
     7741 tggctgccta tttaccacaa tcgctaagcg acctgccgtt tattcccggc tttggcgaaa
     7801 taaaaataga gcgatatggt aaaaaattat tagaaattgt agttagtcat tgccgtttaa
     7861 acaagctgac ttcgcgcatg gaattaataa ataaaagagc ggctaaaaaa agtagtgggt
     7921 caaaacctgc aaaaattgct gagactaaaa aaactacctt gtatatgttt cgttcaggta
     7981 aatctataga agaaattgcc gctgaaagaa attatgccat atccacaatt gaagggcatt
     8041 tatgtgaatt tattttaacc ggtgatgttt ctgtaaatca gtttgtttca cctgaaaaaa
     8101 ttgaagtaat taccaggtat atcaaacatt caacagataa aaaaataacg cctttaaagg
     8161 aaaaattggg tgatgattac tcttattttg aaataagagc cgtattgagt caccttgatt
     8221 atttgttaac ctaaattaat tcaacatttt agttaatagc tctcctgcag catcactaat
     8281 tactgttccg gggccaaaaa taaatgccgc gccggcttca tgtaaaaagt tgtagtcctg
     8341 ttgtggaatt acgccaccaa caacaaccag aatatcgcca cgtccctgtt cttttaataa
     8401 atcgattact tgcggaacta aggttttgtg tcctgcagct aatgaagaaa taccaataac
     8461 atggacatcg ttttcgatag cttgtttcac agcttcagca ggtgttaaaa acaatggtga
     8521 aatatctaca tcaaatccaa tgtcagcaaa tgcagatgca ataacttttg cgccgcggtc
     8581 gtgaccgtcc tgaccgagtt ttgcaattaa aattctcgga cgtcggcctt ccttttcagc
     8641 aaattggtcg ctgagttgtc gggcattgat aaaactttcg ttatccataa cttctttttg
     8701 ataaacacct gccacggttt gaatatttgc tgcatatcgg ccaaatactt tttccatagc
     8761 aaaactaatt tctcccaatg tagcccgctt gcgcgcagca tcaactgcca atgctaataa
     8821 atttccttca ccggttgctg cacagttggt aatttgttgt aaaatatttt ctactgctgc
     8881 attatcacgt tgtgatttca cctgattaat tctttcaatt tgcgataaac gcacagcact
     8941 attatctact tccagaatat cgatatttga ttgttcgtct gtaataaatg catttaatcc
     9001 gataataatt tccgaaccgc catcaatttt cccttgtttt tttgcagccg cttcttctat
     9061 tcgcaatttc ggaatacctg tttctatggc tttttgcatg ccacccaatt gttccacttc
     9121 ttcaattaat tgccaggcat cttcaataat tttttctgtt aatgcttcta caatattact
     9181 tcctccccaa gggtcaaccg ttttacaaat atgtgtttcc tgttgtaaat atatttgtgt
     9241 gttgcgtgca attcgtgcag aaaaatcagt tggaagtgca atggcttcat cgagactgtt
     9301 ggtatgtaaa ctttgtgtgc cacctaatgc agcagctaac gcttcaatac aggttcgtgc
     9361 aacattatta aagggttgtt gttctgttaa actccatcca gatgtttgac aatgcgtgcg
     9421 caacatcaat gatttcgcat tttgaggagc aaattgttgc atcagtttat accataaaaa
     9481 tcgcgcagca cgcaatttgg caatttccat aaaatggtcc atgccgatgc caaagaaaaa
     9541 tgataatcgc ggagcaaaat catctacttt taatccactg ttaatacctg ttcttacata
     9601 ttcaatgcca tcagcaagcg tataagctaa ttcaatttcg ggtgttgcac ctgcttcctg
     9661 catgtggtag cctgaaattg aaatagaatt aaattttggc atattggagg agcagtattt
     9721 aaatatatct gccactatgc gcatacttgg agcaggcgga taaatgtaag tattgcgcac
     9781 cataaactct ttcaaaatat cattctgaat agttcctgcc aattgatgtg gttgcacacc
     9841 ttgttcttct gcggcaataa tataaaatgc cataattgga ataacggcac cattcattgt
     9901 catactcaca ctcatttgat caagcggaat ttgatcaaac aaaattttca tatcctcaac
     9961 agtatcaatt gcaacaccag ccatgccaac atcaccaacc acacgttcat tatcagaatc
    10021 gtaaccgcga tgtgttgcca aatcgaatgc tactgataat cctttttgtc ccgcagctaa
    10081 attttttcta taaaatgcat tcgatgcttc agcagtagaa aatcctgcat attgacgaat
    10141 agtccaaggc tgcattacat acatcgaagt atatggtcca cgtaaaaaag gtgcaagtcc
    10201 gggttgtgta tcaattacat ttttaaaatc atcagtaggc gccacaaaat cgtggttaat
    10261 agaaaaatgt tggtagggaa tatgtttaaa atcgcgtttc attttgttgt agctattgcg
    10321 gttaattatg gcaattattt tgaatcgata aattgttgcc aggcttgctg accaaattgt
    10381 tgtgttaaac tttcaatata aaaactgcca tttgcagcat cgcgataacg gtcgatataa
    10441 ctttcgtatc gcaatatgtt ttgaatattt gttgctaatt gtatggcatt attttctccg
    10501 ctattggaaa atggtgtaac aattaataca tcgcaacctc caagtatagc gctcattgca
    10561 gcagtggtgg tgcgcaacaa attactatgc tcgtctgttt gggtaaacga tgccgtagca
    10621 gttacacaat gtatttcagg attggttata aaatccatct caggcaaata gactttaatt
    10681 aaattatgcc acaaaatttt gaatgccctt aattttgcta tttcaaaaaa gaaatttgaa
    10741 ccaataccaa aacggaaagt acacaattga acgagttctt ctaaaggcac atttcttttt
    10801 tccatctcat caaaataggt ggaagtaatt tgcaaagttt ttaccagttg cgaaacaaca
    10861 tcttcacctt gattggcaaa atgtgtagta tcaattttaa ttaatttgca atcatgtaaa
    10921 tgaccactta agcggaaaaa taaatctgcc aaatgcgcaa acatagttcc attataagta
    10981 tcgttaccat attgttgcca ataaccaatc gggtcaaatt caattccaaa taaaagtctg
    11041 tcgcgttcac cgagatgtcc aactctgtcg cgccatttat tcaccacatc accttctgat
    11101 aaatcgccat acacattaaa aataaaattg gcaggtgttg taagtttgat aatctcattt
    11161 accacatgat cccaaacttc atcattcgtt acactaattc gcacatgact ggcgcctctt
    11221 gctctggcag cagctatcag attattcagg ttgtcgtttt cagttaccaa cacgtcttcc
    11281 gtaatgttcc aggttaacga ttgttgtccc tgatcggaaa ttgcttcaac aaattcgggg
    11341 tctaaatcca gcataaattc agcatcggcg tccacggtaa attggggcag acttttacgg
    11401 gtatacaagg gctgaatggt aatttggtcg atggtattca ccagcttttc gttaaaatcg
    11461 gctcccttca ggtctttggt aacctgttcg aaccattttt cctccggaat ggggcttaca
    11521 tcatgaaata aatctttcat tgttcaaagg tattaaaacc tgtttgtgtt aacgtcttta
    11581 tatttgtgaa aaattatcat gatgcaattg gaaaatttaa caaccgagat aaaaggcaat
    11641 attctatata ttaccataaa ccgtccgaag aacctgaatg ctttaaatat taagaccatt
    11701 gttgaactgg atgaagtgtt tgaagaatat atgtataacg atgatgtgaa gggcgtgata
    11761 ttgaccggag ccggagaaaa atcgtttgta gccggggctg acatttctga gtttgccgat
    11821 tttacacctg ctcagggcaa aacaatgagt gaaaacggac atgatacttt cgataaaatt
    11881 gaaaattata ccaagcctgt tatagccgct gtaaacggtt atgctttagg gggtggatgt
    11941 gagcttgcaa tggcatgtca tttacgtgta gccagcgaaa acgcgatttt cgggcaacca
    12001 gaggtgaatt tgggtataat cccgggatat ggtggcactc agcgactggt tgagttgatt
    12061 ggcaaaggaa aggctttgga actgttaatg accggcgatt cgattaatgc ggcaatggca
    12121 aaagaactcg gattggtgaa tcacgtggtt gcacaggcag atttaatggc aaaatgcgag
    12181 gaaattttga ataagataat gagcaaagga ccggtagctg tggctaaggt gatacagtgt
    12241 gtgaatgcac attacagagg tggtggatat gttggagata gttaccaggg tgagataaca
    12301 cagtttggac aatgtatgga gacggaggat tttgtggaag ggacatcggc gtttttggag
    12361 aagaggaaac cggagtttaa agggaaatga tggaacacgg accccgcggg gcggggcgcg
    12421 gatttggcgg attgacgcgg attttttatg gggtgtgaag aggaacctgc ctggcggcag
    12481 acaggcacgg attgaacggg tggaacggat ggggacggat ttttgggtgg agagatagat
    12541 gtgattaaat tataaaacac aagaggctgt cgttttagga cagcctcttg tgttttgatt
    12601 ataaattgtg ttattgtttt acgaattctt gtgtgattat ttcgttatta gtatttgttg
    12661 tggatagcag ataaaaaccg gaaggaatag attgtaaatt gatagtagtt gaggaggtac
    12721 ttcctatacc tgataaaata atttgtcctt ccatatttat tatttcccaa agttgaattt
    12781 ccccattgat agtaatatat tcaactgctg gattaggata caaagtaatt gccgactgat
    12841 cagaaatcgc ttcttcttta aatattgttg catttaatct ggttaaaaaa atattgtaat
    12901 aaaaatcatt actagttgca ctcatggcgt caaaataaat taacccgtta aacattcctg
    12961 ccaaataaat atttttattt ttatccacgt caacatcgac aatataatcg taggattcta
    13021 cgttatcaaa atcggcaaaa cccagcacca gtcctgtatt cctattaagt gtaacaagca
    13081 gtgcatctgg gtcggcaaat ggattttcaa cggttaatcc atcacactcg gcgtaataaa
    13141 taaaatcgcc tccgcataaa atggtgttac cattattttt aagtgacatg attccggttc
    13201 cataaggtga gaaaataaat ttagcccact caggttccag tgtattaaaa ttaattcggg
    13261 caatgatgga accggttcca tcttctgcat aataatattc gccaaaaacc tgtattgaat
    13321 caacaaaatt aatagcaata gttgcacttc cgtcaaaaat tacatcagat acgctggaat
    13381 atatactgga agtttcgaat tctgttgact gaaacacatc accattttta gtaaaagaaa
    13441 ataagataat atttgtttcg cttatgtctt tgatgagacc atataattca tcaggcccgt
    13501 tgtgaataaa ataactgccg gtgcttttaa atccgagata tgctgtacca ttatttccta
    13561 ctgccatttc ataagcgaga aaattcgcat cacctgtcat agtcctcatc cataccggtg
    13621 aaccatctgc tgtaaattta gccatcagtc cggtgttttc tgttgcatcc ataaattgat
    13681 taacaccatt aaattgcatc tcctgactgt cagaattacc caatacatat acatttcctc
    13741 ccttatcaat ctctacatca taaaatatgt cagagctgtc gccgcctgct gttgcaccat
    13801 ttaaaatatt gccattggca tccaggctca tccaaaatgc atcaatatta ccattagaaa
    13861 tatgggtaac gccgtttata ttcattgtgt cgttgaatcc accgcataaa gtcacacctt
    13921 ttgtcacttt attaaataca atcccggttg gaaaatttgc tccgcttgca ggaatcggct
    13981 ttagccattt taaaataccg tttttatcat attttgccag aaaacaagtc atatattcat
    14041 cgctggttaa ggttatatcc tgtatggtaa gttcataaaa aaaagtgcct gtcatatacg
    14101 tatttccggt tggatcggta gcaatattta ctgtgttagt aggcatacca ttatcgatat
    14161 tctccaaaaa gtgaaatgat tgtccgaatg atgttcccat gattcctaac cataggatgg
    14221 cagataaaat tgtaggtttc attgttgttt atttttagtg caaacctatt tcgggctcca
    14281 gggtctatca agcaaaaaaa tggaggattc tttggtttcg gtgaggagat aaatggtgga
    14341 aactgttgtt gtatttgggt ttggggaact tgtgcggtgg aggactgggg ttgatttttg
    14401 aaatgggatt ggattgaacg ggtggagcct gcctggcggc agatgaggac ggattttttt
    14461 aaaaggaacc tgcctggcgg cagacaggca ctgatttaac agattaaacg gatgaaaacg
    14521 gattaatta
//
LOCUS       DAORKW010000030        14441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_54_length_14441_cov_6.284513, whole genome shotgun sequence.
ACCESSION   DAORKW010000030 DAORKW010000000
VERSION     DAORKW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14441
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54_length_14441_cov_6.284513"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PLB46_02500"
     CDS             complement(<1..761)
                     /locus_tag="PLB46_02500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF75413.1"
                     /translation="MLLLISSFVFAALSIFAFFKQKQVWVYLMVFAATFSIRLYFIQQ
                     DPYLHDWDERYHALVAKNMVEDPFTPMLRTEPVMPYNYQNWWDNHIWLHKQPVFLWQM
                     ALSIKIFGANEFAVRFPSALMCALLALIILRIGYILKKPMVGLSASLLFCFSFFSIEQ
                     TTGALGMDHNDVAMLFYVSASIWALMEYTLSGKKRFLILIGLFSGMAILVKWLTGLLV
                     YACWGAYLFFANSSNITSKSKQLTHALLALAITCVV"
     gene            complement(836..3766)
                     /locus_tag="PLB46_02505"
     CDS             complement(836..3766)
                     /locus_tag="PLB46_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRF75414.1"
                     /translation="MLRSIILLPLLLSLSIYAQIPQRWEIDAKTFRANSDQVETGDMD
                     SDGDLDIVVGGGDYYHMMWFENTGTTFSTKQHVLDIVADRFMLSDINGDGYLDIVSSH
                     SWYIELTYNLNISGTGLDFEEHTIYAPLIGAYSFPFTVADLDSDNDDDILYTTTGAHE
                     LIWAENVYAEGGEFIPHGIFELTYYPNQYAVGDFDNDDDMDIITSTLDDNVCWIENLG
                     GEFFSFETIIEEIENMKCMTFTDLQDDNDLDLLIGAGESYDDDDNGLFVLYNDGLGNF
                     SGAVNYYTYTDVINITTGFINDDIYPDVVFLDNSLGALDGYPVNGHMLYGTNDATLSD
                     INNLLSFTPPQFDLTGFSKGDLSDINEDGFDDLAISVNGGVIWSTNVPAEGHFSGYKQ
                     LAQMRAEPRGLSFIDENGSDENEIILYDRNGRINSYDFDTLANAFELISKPDSVAKLF
                     NVSSLLTFDFDNDGLEDIFANSYSACEECLYDEEYKILWVYKNLPGQGVYASPSIAAT
                     EKLIYPITSDIDGDGYKDIVASYPNYYFVGYDDDGFEIYASQYNIYWLKNESGSGIFT
                     KHMISLEDTPTQNITKSGDMDEDGDMDVISVNYATGNLGWWENLDGLGSFSDIQDLIE
                     IDSIRAFDIIDEDNDGDTDILLFLNNALFISLNETGVFTDTLLRSSITDGYYAGTPLV
                     EDMDSDGRQDVISGSFQTYYHHNTALTGIDSIPAVMPFNYNYTIAADINEDGIAEIVG
                     VPDEDYFPGDSAFAIEINPEPVYVVVEHSNDSLTLDENLFYTDTITVHLNTIPAETCY
                     MEFNAKAEFDPEYEVTFTGADPHVFTMIFNPDSTALIPQTIVITAENDYEMDSADRGK
                     LFTEAYSDHNIYDFLFDGLTYFEILDNDYEEVSNQDKNEALHIMPYYNEAEQNLYITY
                     NALSSLNTINLYTIDGKIITGINTNQSSGTVSINCSGFAEGLYLIEFIDKKNNLRVIR
                     KVVF"
     gene            complement(4136..4432)
                     /locus_tag="PLB46_02510"
     CDS             complement(4136..4432)
                     /locus_tag="PLB46_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRF75415.1"
                     /translation="MFTPLPFCVYVLFSEKDNMLYTGYTSDLNRRMQQHLNGESPNTS
                     KRLPVKLIFCEYYLFKEDAERRENYFKTTAGKKALKIMLNGTLEKLKNGFTNNL"
     gene            5197..6420
                     /locus_tag="PLB46_02515"
     CDS             5197..6420
                     /locus_tag="PLB46_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRF75416.1"
                     /translation="MATVKIVLRQKESKNGTFPLALRITKDRKTSFIHLGHSIREEDW
                     DANQQRVKKSYPNSARLNNFLVKKLSEATDKSLELETHKSSVSSFAVKQKIKPTGGLT
                     LFAQADLYLDNLKTAGKYNRYTADKPRVKHFKDFLKGNDISFSDVTIPLLERFQIYLK
                     STLSISERTIVNHLVVIRSVFSQAIKAEIIDQKYYPFGASKIRIQFPQSSKIGLDTRD
                     IQALENLHLQENTFENHCRNLWLFSFYLAGMRVSDILRLKWTDIQNDRLHYVMGKNSK
                     AGSLKIPEKALYIISQYSSVRRFKDDYIFPDLKVLDSTTEPFIVQRKIAFTTSRIDKF
                     LRNKVAPQAKIQKRLTMHIARHTFGNISGDKIPIQMLQKLYRHSSVLTTIGYQSNFVN
                     KDVDEALESVINVSK"
     gene            complement(6435..7196)
                     /locus_tag="PLB46_02520"
     CDS             complement(6435..7196)
                     /locus_tag="PLB46_02520"
                     /inference="COORDINATES: protein motif:HMM:NF022019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRF75417.1"
                     /translation="MDIYDFIYPISEKQILELAKVNGPCNNVVVGIDKHCFGVTFGES
                     GDICQFLYVNGTIQLKGYLLQIHLAEKNFYPLVVEFTYMMTIEFDLIDPVDLYNALIK
                     NFRSAIEIQGIQTVTNFELRLLNEKFGDNYLISAGPIMVIYYLNKLEFYQTIVGDNHL
                     AIEADKNYVYLLINCRNGLFKIGRSKNPLQREKTLQAEEPEILLIKYWAAEKAFENTL
                     HKHYLSKRIRGEWFKLNLNDLLELRKVTNELNLSK"
     gene            complement(7364..8575)
                     /locus_tag="PLB46_02525"
     CDS             complement(7364..8575)
                     /locus_tag="PLB46_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75418.1"
                     /translation="MIIKLVPIKTKAKLSNVINYIATDKGRIEDYKNQGVFHNVKSLE
                     LENLRKEFEVNYDNYANKRSNGNIAMHIIQSLSPLDKDTMTIEMMDDITSTFLNMAYP
                     KALGFGTHHKEQSHWHTHTIVSANELMSKQSTRLSKEKLFEVHKSMLDYVHEKYPELK
                     TNIDISNWGKKLHNENDYYMKKRNPNFKLTKEEVQEKVQDIFRTCESSKQFYAVLEFD
                     GFKTYTYKDNVYGILFGEDEKKLRFSRIGIDQEQIAELDKQYERLQELEKLSSGNKTM
                     TLDHYTYDLNDMRGNPDGQDPDIKTDEEIELEEAILNAEFAEIMNYPIEEQEVIKTGE
                     IEKEVATEEQEIEVIDNEITADTEATMSDDPDPSEEQEPNDQENDIEDLKSDSQSDIA
                     TDNDMDMGTEP"
     gene            complement(8572..8967)
                     /gene="mobC"
                     /locus_tag="PLB46_02530"
     CDS             complement(8572..8967)
                     /gene="mobC"
                     /locus_tag="PLB46_02530"
                     /inference="COORDINATES: protein motif:HMM:NF017524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HRF75419.1"
                     /translation="MESNEKQKTLWREQKRIYRQKRKEYSLILTNHEAAELKKFAQQK
                     KMKVPAFLKSLIKAYSKKTEYILPDDNTLQNLILEMRKIGTNINQMAKYVNTNKTISK
                     SEMEGFRVKLDDLEHLIVSKLTQPVKKIT"
     gene            complement(8954..10405)
                     /locus_tag="PLB46_02535"
     CDS             complement(8954..10405)
                     /locus_tag="PLB46_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRF75420.1"
                     /translation="MIGFIDFCISLIGSLFSGLWHLTELVISLWSKKKGYDATFTPLH
                     EILSKHNKGFSLGAHQSMDLKTTFQHLLVSAPSGVGKSTSVCIPSLINLHGNGSVVVL
                     DPAKELYKAVGDVYKDYNTYVINFSDRTGSESDGWNPMPENVADLPNFTDRISNIVHG
                     SSRDKFWQIQSAELLTTLAEPLYKLDKKYRTVPNLMLLLNTLAAKEDLCNKFMAKYAS
                     PPTWLEYLAFHKNSDNTKASILSSAKATLSVFRQEHIQRITNTSTFSISELRKQKSIV
                     FIQTNPAELEKYKLLISEFFNQLIGYILADMPDEADENIFLLLDEAGVYRIEALPLGI
                     VQARKMRCGIMMLVQSEQQLYNLLSKEEASTIISNCSRLILTNAPLSTAREISELAGK
                     FQYKDEENITRVRELISVDEVRSMKADQGILVSGHFRPSLITLKPHYKDKKYKAITSV
                     APPTLIRKLPENNIELLPIESLVQQQTKSNGIK"
     gene            complement(10574..11281)
                     /locus_tag="PLB46_02540"
     CDS             complement(10574..11281)
                     /locus_tag="PLB46_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75421.1"
                     /translation="MKSMQLFSTLALIGAVCCSSCKETSIAQETVILIDVTDTAIQQF
                     DTTGVNNVSIMDDSPLNGETTKVVIISDVAVNKHFEASIPIVTSQLFSNTKSRKKMVT
                     EYLSKLNTMIGNVTEQDKGKDASVIYKRIAEELNILTKKHAIKKTMIINSDLMEKSFI
                     NFYKESVLELMVTNPDSLSNKLTGKYPLKDLTGIEIFILFQPNDVEESERFEVVSTFY
                     QNLFESHGATVHIQSNI"
     gene            complement(11304..12284)
                     /locus_tag="PLB46_02545"
     CDS             complement(11304..12284)
                     /locus_tag="PLB46_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75422.1"
                     /translation="MKKFTKFNFGLQVHELEAYISKQVKRIITSALNDINAAIKTKLE
                     NLQGISNAITAEFNVVREKVGQWLQPEIEKLTEKINQFETSGIIDALAAKIDEAKQAS
                     KSLIADISRTVLTYNWKWYFICWVAIFAINMVDSIFNAPIYQIIVTTLLFSIIVSVFI
                     AGILMYFSHLVGTKARIANTPKQRMLRLLFGIASAAIIFLILGFIRKAYTGEENLFVN
                     SPFVWMILNTFFFTVAVLISYYKMPTEEQKLEHDALKAKKKELAKLQLIVKIAERDMT
                     AAVEEYNATKQRREEFISYRDKCLSSLDKEEALVIATCKKEAEIKGGTFN"
BASE COUNT         4461 a         2653 c         2405 g         4922 t
ORIGIN      
        1 acaacacagg ttattgccag cgctaataag gcatgtgtaa gttgcttcga ttttgatgtg
       61 atattgcttg aattggcaaa aaataaataa gctccccaac aggcatacac taaaagtcct
      121 gtaagccatt ttaccaaaat tgccattcct gaaaataatc caataagtat caaaaaacgt
      181 ttttttccgg ataaggtata ttccattaat gcccatatac tggcggaaac ataaaatagc
      241 atagcaacat cgttatgatc catcccaagg gcgcccgtag tttgttctat agaaaaaaag
      301 gagaaacaaa acaggagtga tgcggaaagt cccaccatag gtttttttaa tatataccca
      361 attctcaaaa tgattaacgc taataaggca cacataagtg cacttggaaa gcggacagca
      421 aattcatttg caccaaatat tttaatacta agtgccattt gccataaaaa cacaggttgt
      481 ttatgcaacc agatatgatt gtcccaccag ttctgataat tataaggcat aaccggttcg
      541 gtgcgcaaca ttggggtaaa cgggtcttcc accatgtttt tagccactaa tgcatgatat
      601 cgttcgtccc aatcgtgtaa ataagggtct tgttgaataa aatacaatcg tatactaaaa
      661 gtagccgcaa aaaccatcaa atacacccac acctgcttct gtttaaaaaa agcgaaaatg
      721 cttaaagctg cgaaaacaaa tgaggagatg agtagaagca tggaatgatt tagccgaaac
      781 aaagataaca gctaatctta gataaatcat tataaacgac gatacgaatt aaatattaaa
      841 aaacaacttt ccttattacc ctcaaattgt ttttcttatc tataaattca atcagatata
      901 atccttcggc aaaccctgaa caattaatac ttactgttcc tgaagattga ttagtattta
      961 ttccagtaat tattttacca tcaatagtat acaaattgat agtgtttaat gatgaaagtg
     1021 cattgtaagt gatgtataaa ttttgttccg cttcgttgta atagggcata atatgtaacg
     1081 cctcattttt atcttgattt gatacttcct cataatcgtt atctaaaatt tcaaaatagg
     1141 taagtccatc aaacaaaaaa tcgtaaatat tgtggtcgct ataagcttct gtaaacaatt
     1201 tgcctctatc ggcactatcc atttcataat cgttctctgc ggtaataact attgtttgtg
     1261 gaattaaagc agttgaatcg ggattaaata tcatggtgaa cacgtgagga tcagcacctg
     1321 taaatgtaac ttcatattcg ggatcaaatt ccgcttttgc attaaattcc atatagcagg
     1381 tttctgccgg aatagtattg agatgaactg ttatggtatc tgtgtaaaaa aggttttcat
     1441 ctaaagtcag gctgtcgttg gaatgttcaa caactacata taccggttcg ggatttattt
     1501 caatggcaaa ggcagaatcc cccggaaaat agtcttcatc tggcactccg acaatttcag
     1561 caattccatc ttcattgata tcggcagcaa ttgtataatt ataattaaaa ggcattacag
     1621 ccggaataga atcaatgccg gttaaagccg tattatgatg gtaatacgtt tgaaaactac
     1681 cggaaattac atcttgacga ccatcgctat ccatatcttc taccaacggg gtgccagcat
     1741 aataaccatc agttatactg cttctcaaca aagtatcggt aaacacaccg gtttcattta
     1801 gactgataaa taatgcgtta tttaggaaca ggagtatatc agtgtctccg tcattgtctt
     1861 catcaataat atcgaatgca cgtatggagt ctatttctat caagtcttga atatccgaaa
     1921 agctaccaag accatctaaa ttttcccacc aacctaaatt tcctgtagca taatttacag
     1981 aaataacatc catatcacca tcttcatcca tgtcgcccga ttttgtgata ttttgtgttg
     2041 gggtatcttc taaggaaatc atatgtttgg taaatattcc gcttccactt tcatttttca
     2101 accagtaaat attatactga gaggcataaa tttcaaaacc atcatcgtca taaccgacga
     2161 aataataatt tggataacta gcaacaatat ctttatatcc atccccatca atgtctgaag
     2221 ttatcggata aatcaatttt tctgttgctg ctatagaggg gctggcatat actccttgtc
     2281 cgggtaaatt cttgtaaacc cataaaattt tatattcctc atcatataaa cattcctcgc
     2341 aagctgaata gctatttgca aaaatatctt caagaccatc attgtcaaaa tcaaatgtta
     2401 atagtgatga aacattaaac aattttgcaa cactatccgg ttttgaaatg agttcaaaag
     2461 cattagctaa tgtatcaaaa tcataactgt ttattctacc gtttctatca tacaatatta
     2521 tctcattttc gtcgctaccg ttttcatcaa taaagctcaa tcctcttggc tcggctctca
     2581 tttgtgctaa ttgtttatat cccgaaaaat ggccttcagc cggcacattt gtcgaccaaa
     2641 taacaccacc atttactgaa attgccaaat catcaaaacc atcctcatta atatctgata
     2701 aatctccttt tgagaagcct gttaaatcaa attgcggtgg tgtgaagctc aacaaattat
     2761 taatgtcact taaagttgca tcattggtgc cgtacagcat atgtccatta actggatatc
     2821 catctaacgc tcctaaacta ttatccaaaa aaacaacatc tggataaata tcatcattga
     2881 taaacccggt tgtaatattt attacgtcgg tataggtata gtaattaaca gcacctgaaa
     2941 aattacccaa accatcatta tacagcacaa acaatccgtt atcatcgtca tcgtaggatt
     3001 ccccggcgcc tatcagcaaa tccaaatcat tatcatcttg gagatcagta aaagtcatac
     3061 atttcatatt ttcaatctct tcaataattg tttcaaatga aaaaaattct ccacccaaat
     3121 tttcaatcca acatacatta tcatctaaag ttgaagtaat aatatccata tcatcatcat
     3181 tgtcaaaatc tcctacggca tattgatttg gataataagt aagttcaaaa ataccatgtg
     3241 gaataaattc acccccttca gcataaacat tttcagccca gattaattca tgtgcaccgg
     3301 ttgtggtata taagatatca tcatcattat cagaatccaa atctgcaaca gtgaatggaa
     3361 aactataagc cccaataagc ggagcataaa tagtgtgttc ttcaaagtca agccctgtgc
     3421 ctgatatatt taaattatag gttagttcaa tataccaact atgagagctt acaatatcta
     3481 aataaccatc tccatttata tcgctcaaca taaaacgatc tgcaacaata tctaaaacat
     3541 gttgcttggt tgagaaggtg gtacctgtgt tttcaaacca catcatatgg tagtagtcgc
     3601 ctccaccaac cacaatatcc aaatctccat cagaatccat atcgcctgtt tccacctgat
     3661 cagaatttgc cctgaacgtt ttagcatcaa tctcccatcg ttgtggaatt tgggcataaa
     3721 tacttagtga aagtaataag ggcaataata ttattgatct tagcaagtag ggaaaatgaa
     3781 acatgggctg tattttaatt tcaaatgtag gtagtcctgc tcagaaatgc ttatggtttt
     3841 gagcgaaata caggtttaag tgtgcgaagt gtgtggtatt tttagtaaag gggatatttg
     3901 gggagatgaa tgagtctcgt ggctatgcat tggaagagat tcttggagtg gggtgttata
     3961 gtaaaaaatg tgggaggtta ttctacgacg agaaagcttc cgttcgtctt aaatacggga
     4021 caaaactttt aggcgctaac cacttctcct tattttctta atttgcatcc agtttggttt
     4081 agctgtgaca agctatttca ggacgagaca gaatgtttaa ctcgtataaa ttatactata
     4141 gattatttgt aaatccattt tttaatttct ccaaagtacc attcaacata atttttaacg
     4201 cctttttacc tgcagtagtt ttaaaataat tttctcttcg ttcagcatct tctttaaata
     4261 aataatattc gcaaaatata agtttaaccg gtaagcgctt tgaagtattt ggactttcac
     4321 cgttgaggtg ttgttgcatc cttctgttta aatcggatgt atatccagta tataacatgt
     4381 tgtccttttc actaaaaaga acgtacacac aaaaaggtaa gggagtaaac atttttaagg
     4441 aattttaata aatgtagaaa tgattttgga ggagttcaaa ttttccctga agtagcgctc
     4501 ctgcgtcgct catttcaggg gtgaacccct tttgtcgctc actctgaagt accccggaag
     4561 tagagctcgt gcttgccagg ttataaggtt tatattaaat tttccaaaga atttgagctt
     4621 gtgttttgga actgcttgtg cagagtgctt ttgggataaa aaaaattaac gtctccggaa
     4681 gccccagaag tatagctcgt tcctcgctca cagcggggca ccctaaagtg aaaaaacctg
     4741 atgcgttatg ttgtataatc atattttgaa ttccgaaagc cctaaagtag aaaaagcctg
     4801 atgcgttatg ttgtataatc ctattttgaa ttccgaaagc cctaaagtag aaaaagcctg
     4861 atgcgttatg ttgtataatc atattttgaa ttccgaaagc ccagaagtag agctcgttcc
     4921 tcgctcactt cggggcaccc taaagtgaaa aagcctgatg ctcctgcatc aggctttttc
     4981 tactttaggg tggtgtgggg cgggatcgaa ccgccgacac aaggattttc agtccttggg
     5041 atattggttt gatatgtatt gatgtgtgtt gaaacaccta taaatactga agcacgttta
     5101 aataatattt cataaaaata catataaaat cacatattgt tgtacctatg ttgtaccttt
     5161 gtaacatcaa ttcatccctt aaacccttta ttatctatgg ctaccgtaaa aattgtactg
     5221 agacagaaag aatcaaagaa cggaactttt cctttagcat tacgaataac caaagataga
     5281 aaaacgtcat ttattcattt ggggcattcg ataagagaag aggattggga tgccaatcaa
     5341 caacgtgtta aaaaaagcta tcctaattct gcaagattga ataactttct tgttaaaaag
     5401 ttatcagagg ctactgataa atcgttggaa ctagaaactc ataaaagtag tgtatcatca
     5461 ttcgcggtaa agcaaaaaat aaaaccgaca ggtgggctaa ctctttttgc acaggccgac
     5521 ctttatcttg ataacctaaa aactgcagga aaatataacc gatatactgc tgacaaacct
     5581 agagttaaac actttaaaga ttttctaaaa gggaatgata tttctttctc agacgttacc
     5641 attccacttt tagaaagatt tcaaatttat ttaaaaagca cattatcgat aagtgaaaga
     5701 acaattgtaa atcatctagt tgttattcgc tcagttttta gccaggcaat caaagctgaa
     5761 attatcgacc aaaaatatta cccatttggg gcctcaaaga ttcgaattca atttcctcaa
     5821 tcctccaaaa ttggtcttga cacaagagat attcaagcat tggagaattt acatttgcaa
     5881 gaaaatactt ttgagaacca ttgccgcaac ctttggctat tttcttttta tctagctggt
     5941 atgagagtgt ctgacatttt aagactaaaa tggacagata ttcaaaacga tagattacac
     6001 tatgttatgg gtaaaaattc aaaggctggt tcactcaaaa ttccagagaa ggcgctttac
     6061 atcatttccc aatattctag tgtaaggcgt tttaaagatg attacatatt cccggactta
     6121 aaagttttgg attctacaac tgaaccattt attgttcaac gcaaaatagc ttttaccact
     6181 agtaggattg ataagttttt acgaaataaa gttgcacctc aagctaaaat tcagaaaagg
     6241 ttaaccatgc acattgcacg gcacacattt ggaaatattt caggtgataa aattccaatc
     6301 caaatgctac agaagcttta tcgacattca tccgttttaa caactattgg gtatcaatcc
     6361 aattttgtta ataaagatgt tgatgaagcc ttagaatccg taattaatgt ttccaagtaa
     6421 aaagttggaa acgattattt cgacagattt aattcattcg taacctttct taattccaat
     6481 aagtcattta aatttaattt aaaccattca cctctaatac gttttgagag gtaatgctta
     6541 tggagtgtat tttcaaatgc tttctctgct gcccaatact taatcaacaa tatctcaggc
     6601 tcttctgctt gaagggtttt ttccctttgc agaggattct tacttcttcc tattttaaat
     6661 agcccgtttc ggcaattaat tagcagataa acataatttt tatctgcttc aatagctagg
     6721 tgattatctc caacgatggt ttgataaaat tccaatttat ttaaataata aattaccata
     6781 atagggccag cagagattag gtaattgtca ccaaatttct catttaacaa acgcaattcg
     6841 aaatttgtaa cagtttgaat tccttgaatt tcaattgccg aacgaaaatt tttaataagc
     6901 gcattgtata agtctacagg gtcaatcaaa tcaaattcaa ttgtcatcat ataagtaaac
     6961 tctactacta aaggataaaa attcttctca gctagatgga tttgaaggag atatcctttt
     7021 aattgaattg tcccatttac ataaagaaat tgacaaatat ctccagattc accgaaggta
     7081 acaccaaaac aatgtttatc aattcctaca acgacattat tacaagggcc attgactttt
     7141 gcgagttcaa ggatttgttt ttctgaaatt gggtatataa agtcataaat gtccatataa
     7201 tttactttca attttctttt gcaattatta aagatattaa ataattccat tacatgatta
     7261 catattaaaa cctaattgca agatagcttt cagactgtct ttttatcttt cgtaaaagat
     7321 aaggttgtct gaaagcgatc ttggcaggaa tattcctgca cctttatggc tcggtaccca
     7381 tatccatgtc attgtctgtt gcgatgtcac tttgtgagtc ggacttcaag tcttcaatat
     7441 cattttcttg gtcatttggt tcttgttcct cggaggggtc tgggtcatcg ctcatagtgg
     7501 cttcagtgtc agcggttatt tcattatcta ttacttcaat ttcttgttct tccgttgcaa
     7561 cctccttttc tatttcaccg gttttaataa cctcttgctc ttcaattggg tagttcataa
     7621 tttctgcaaa ttccgcattt aaaattgctt cctccaattc gatttcttca tcggttttaa
     7681 tatcagggtc ttggccatca gggttgcctc tcatgtcgtt taaatcataa gtatagtggt
     7741 caagggtcat tgtcttgtta ccagaactta acttttcaag ttcttggagg cgttcgtact
     7801 gtttatctaa ttcagctatt tgctcttgat caataccaat tctggaaaaa cggagtttct
     7861 tttcgtcttc gccaaataag ataccgtata cattgtcttt atatgtatag gtcttaaagc
     7921 catcaaattc gagaactgca taaaattgct ttgatgactc acaggttcga aaaatgtctt
     7981 gtactttttc ctgcacctcc tctttggtca acttaaaatt tggattgcgt tttttcatat
     8041 aataatcatt ttcgttatgc aattttttgc cccagtttga aatatcaata ttggttttta
     8101 gttcaggata cttttcatga acgtagtcca acatactttt atgaacttca aaaagctttt
     8161 cttttgaaag ccgggttgat tgtttgctca ttaattcatt agccgaaaca attgtatgtg
     8221 tatgccaatg tgattgctct ttatgatgcg ttccaaatcc gagtgctttg ggatatgcca
     8281 tattcaaaaa ggtagaggtt atatcatcca tcatttctat agtcattgta tccttgtcta
     8341 atggtgataa actttgaatg atgtgcatcg caatatttcc attactcctt ttatttgcat
     8401 aattgtcata attaacttca aactcttttc gcaagttttc taactccaga cttttgacat
     8461 tgtgaaacac cccttggttt ttataatctt caatccgccc cttgtcagtt gcaatgtaat
     8521 taatcacatt tgatagttta gcctttgttt taatcggaac aagcttaata atcatgtgat
     8581 cttttttaca ggttgagtta atttcgaaac tatgagatgt tcaaggtcat ctaactttac
     8641 acgaaatcct tccatttcac ttttcgaaat ggttttattt gtattcacat attttgccat
     8701 ttgatttatg ttagtgccaa ttttccgcat ttctaaaata aggttttgga gcgtattgtc
     8761 atcaggtaaa atatattcag tttttttgct atatgctttt attagcgatt ttaagaaagc
     8821 aggcactttc attttctttt gctgtgcaaa ttttttaagc tctgcagcct catgatttgt
     8881 caatatcagg gaatactctt tccgcttttg gcgatagata cgtttttgtt ctcgccacaa
     8941 tgttttttgt ttttcatttg attccattgg attttgtttg ttgttgaaca agagattcga
     9001 tgggtaacaa ttcaatattg ttttccggca acttccggat tagagttgga ggtgcgactg
     9061 atgtaattgc tttatatttt ttatctttgt agtgtggttt tagtgtaatg agtgacggac
     9121 gaaaatgccc tgaaactaag attccctggt ccgccttcat tgaccttact tcatccactg
     9181 aaatgagttc tcgtacccga gttatatttt cttcatcttt atattgaaat tttccggcga
     9241 gctcagatat ttctctagct gttgacagtg gcgcatttgt aagaataagt cgtgagcaat
     9301 tagaaattat ggtagaagct tcctctttag ataaaaggtt atataattgc tgttcagatt
     9361 gcactaacat cataatgcca catcgcattt tccttgcctg cactattccg agaggcaaag
     9421 cttctatgcg atatacacca gcttcatcaa gtaataaaaa aatgttttca tctgcttcat
     9481 caggcatgtc agcaagaata tatccaataa gttgattgaa aaattcactg ataagtagtt
     9541 tatatttttc aagctctgca ggattggttt ggataaaaac aatcgatttc tgtttcctca
     9601 gttcagaaat cgaaaaggtg gaggtgttgg ttattctttg aatatgctcc tgtctaaaaa
     9661 ccgataaggt tgcttttgca ctgcttaaaa tactggcttt tgtattatca gaatttttgt
     9721 gaaatgctaa atattcaagc catgttggag ggctagcata ttttgccata aatttattac
     9781 aaaggtcttc tttggctgcc agcgtattca gtagcaacat aagatttggc actgtcctgt
     9841 attttttatc gagtttatac aagggttcag cgagtgtcgt taataattcc gcactttgaa
     9901 tctgccagaa tttatcacga gaacttccgt gaacaatatt cgaaatgcgg tctgtaaaat
     9961 ttggtaaatc agctacattc tccggcattg gattccaacc atcgctttca gagccggtcc
    10021 gatctgagaa atttataaca taagtgttgt aatccttata aacgtcccct acagccttgt
    10081 aaagctcttt agcggggtct aggacgacaa cactaccatt tccatgtaaa ttgattaacg
    10141 atggtataca gaccgatgtg gactttccta caccacttgg tgctgatacc aataaatgct
    10201 gaaaagtggt ttttaagtcc attgactgat gcgctcccaa actaaaaccc ttattatgct
    10261 tactgagtat ttcatgcaaa ggtgtaaacg ttgcatcata tcctttcttt ttgctccaca
    10321 gtgaaatgac aagttcggta aggtgccaaa ggccgctgaa aagactacca atgagactaa
    10381 tgcaaaaatc aataaatcca atcatggcaa tttgtttgag gtgagaattt gctttaaaaa
    10441 aattgacagg tgttcagaaa taatagccac aagcttggtg agtgcataac cagctaatac
    10501 aataatatat attactagtc gtatgcattt caccaatatg tggcttactt tatttctgtt
    10561 gctcatagtg cttttaaata tttgattgaa tgtgtactgt tgcaccatgg gattcaaaaa
    10621 gattttgata aaaagtggag actacctcaa atctttcact ttcctctacg tcattgggtt
    10681 ggaataaaat aaatatttca atcccggtca aatcttttaa tggatatttt ccagttaact
    10741 tattgctcaa tgaatctgga ttggtaacca tcaattccaa cactgattct ttgtaaaagt
    10801 taatgaaaga cttttccatt aaatctgaat taataatcat tgttttttta atcgcatgct
    10861 ttttggtcaa tatatttagt tcttctgcaa ttcttttata tatcactgaa gcatctttac
    10921 ctttgtcttg ttctgttaca ttgccaatca ttgtgttcag cttcgaaagg tattcggtta
    10981 ccattttttt tcgtgacttt gtattgctaa aaagctggct ggttactatt gggatagatg
    11041 cttcaaaatg tttattaact gcaacatctg aaataattac caccttggtt gtttctccgt
    11101 tgagcggtga atcatccatt atcgaaacat tgtttacacc tgttgtatca aattgttgta
    11161 tagctgtgtc agtaacgtca attaggatga ctgtttcttg tgcgattgat gtctctttgc
    11221 acgaagagca gcacaccgcc cctatgaggg caagtgtgct gaataattgc atcgatttca
    11281 taaaactctc agaattgatt tggttaatta aatgttcctc ctttgatttc tgcttccttt
    11341 ttacaggttg caatgacaag cgcttcttct ttatcaagcg atgaaagaca tttatcacgg
    11401 tagctgataa actcttctct tcgttgcttt gtagcattgt attcttcaac tgcagcagtc
    11461 atatcgcgtt ccgcaatttt cactatcaac tgcaactttg ccaattcctt ctttttagct
    11521 tttagagcat cgtgctccaa tttttgctct tcagttggca ttttatagta ggatattaat
    11581 acagcaacag taaagaaaaa tgtatttaat atcatccaaa cgaatggtga attcacgaaa
    11641 agattttctt cacctgtata tgcttttctg ataaatccta atatcaagaa aatgatggct
    11701 gcgctagcaa tgccgaatag gagtcttaac attctttgtt ttggtgtgtt agctattctt
    11761 gctttcgtac caactagatg cgagaaatac attaatattc cagcgataaa aaccgagact
    11821 ataattgaaa acaaaagtgt ggttactatt atttgataga ttggggcatt aaaaatagaa
    11881 tccaccatgt taattgcaaa aattgctacc caacatataa aataccattt ccagttatac
    11941 gtgagcactg tacgggaaat gtcagcgatg agtgattttg aagcttgctt tgcctcatct
    12001 atcttagctg ctagtgcatc aataataccg cttgtctcaa actggttaat tttttcagtt
    12061 agtttttcaa tctctggttg taaccattgc cctacttttt ctcgtaccac attaaattcc
    12121 gctgtaattg cattactgat accttgtaaa ttttcgagtt tcgttttaat agcagcattt
    12181 atatcattca aggcgctagt aatgatgcgc tttacttgtt tcgatatgta ggcctcaagt
    12241 tcatgaacct gcaggccgaa attgaatttt gtaaatttct tcataactat tattattaaa
    12301 aggtgaatga attaagaaca cgaaggggta aattggattg tcaatcagtt agtgattccc
    12361 aaaaaggcgg tgacccccgg caacaatagt ttgagttttc cacagaaaac cacagattga
    12421 aatggtgtaa gtagacagtc agatttgtcc attgggcata aaagcctcta aatgaaccgt
    12481 tttggaggtt tttgtagttc ataagccttg gttttaggcc gaaccccctg tgcatagtat
    12541 ttttaccaaa agtgtgatac gatactaacg aattagtatc tttgtaccag ttcaaaatgt
    12601 cattcgtcta ttttcgacga tatcgaatga cacatctttt ggtcgatttc ttttcttgca
    12661 agaggggtcg acttttttta tttcatatta ttcgatttct ttttacaatt tgagattaaa
    12721 aaagccactc aaaattttga gtggcttact ttaaaattta tttaacataa atttatttca
    12781 gcctattcgg tctctggttt gaactcttgt tgttttggtt gaaatagtca ttacggtaaa
    12841 attgtttagt gtatattact atgatgtaat tttattgttt ggaaaataaa aataccgcca
    12901 ctctttattt ttaccaaatt attcaggtag atgttattaa caccgtcctg tgtgtaaaat
    12961 gtattaaaat ggacaacata tcagaaaccc catcggcact aagcatgaca attttttata
    13021 taactaacga acagaaaatg ttgtaaggat tgtagagttg agcgtaattt ttatttaccg
    13081 atggtaaaaa tttaaatatt tcgaataatt atgggctttc tcttgtcgat tcagtaacta
    13141 tatgactaaa cttccttcga ttttttgcat aaaatgtaaa aagtgctatc tctttctaac
    13201 tactttaaat aaaataattt tcttgttagc cggggaacaa tcacttcatg taatgagggt
    13261 aaacttgcga tgagtagtaa attccttgta aaaaggtgaa ctatcttcta tctatatgcg
    13321 ctcataaaat cacctccgga gccacaaaaa atgtcaaaca ccggaaaaat ttcacgcttt
    13381 caattttttg caaaaaaagc aactgagcca tatttttttg ccgttttaat aatttgtaaa
    13441 actaatagct ttaagcaaaa aaataattga ttgcattttt gttaatgaat tcactatctt
    13501 tgtgcaaaga aaaagccccc tttggacggg agctcaattc aaagtgttac cctggaagtt
    13561 tcaagtttgg accttggaac tggacacacc ggcgtagcac tttttctttt gtaaagataa
    13621 tcattacaat taattttttc aaaaaactaa tgcgatagaa aaaattcaat tacatagcaa
    13681 ataaacacta tagttgttga tgcaaattaa tgttatccat tgaaatgccc agccagaagg
    13741 tttttaagtt gctatttatt ttaatatttt gtaataatat ttttcaaagt tgtgatttta
    13801 gcgacttaaa aatcttactt acatacgtta ttttcttctc gcttgaagcg ctaaaatctc
    13861 ccccccccaa aaaaatttaa gcaaaatctt ttttattgaa ttttttcaat ctttcttaaa
    13921 tctttcctct atttcaaaat ttctaaatct tttcaagagg aacattcttt cttttgtagg
    13981 gatattatgt ttcttaaatg taagaatgca actcatcaat gcttctatga caaataacaa
    14041 tctctttcaa agatcgaaaa gtattcatag tctttgaact tcaatatttt cttactatgc
    14101 tcggaagttt tattaaaact tggacaaaag gagttcggct ccatgccttc gaaaaaatca
    14161 cgccaaacca atacaggagt agaggctaca cctgtattgc acacttgtgt ttaagagttt
    14221 gtcatgattt tacctcagtc atgtagcctc acacgcagcc tcctttgtcc tcaaggtcgc
    14281 tttactcata tcgttcattc cacttcaggg gcttccgcaa gctccaactc ccttcgtgtc
    14341 attctctcat ttcgtttcag ccaccttgag tccaccggct gctggttagt ttagctcgcc
    14401 tgcgcagtcg aatgggctgc ggggttgtca tggtttttgg t
//
LOCUS       DAORKW010000031        13875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_59_length_13875_cov_5.096889, whole genome shotgun sequence.
ACCESSION   DAORKW010000031 DAORKW010000000
VERSION     DAORKW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13875
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59_length_13875_cov_5.096889"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2460
                     /locus_tag="PLB46_02550"
     CDS             <1..2460
                     /locus_tag="PLB46_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75423.1"
                     /translation="DRNFESAENEEEETAVDEWLYAQKAFPYGVADKHAQRNAIAHMQ
                     SARAQNALFKTTADGDDWSFIGPYNIGGRILDVDCPGGNKNIIYVATASGGVLKTYNG
                     GDTWQNIFDTESTLSIGDIAIDPVDTATIYIGTGEPGNGVGSVTYDGNGIYKSTNSGA
                     TWTNIGLSEGGNTGRIAINPDNTQIIFAAMLGDLFQNTPDRGIYRTENGGTTWEKVLY
                     VNDSTGGIDVAINPENPNIIYASTWERVRRYNRRDYAGVGSRIYRSEDGGDTWEMLST
                     GLPFGSTELSRIMLSIAPSDPDIVYASIVASNDKLKDIYKSTNGGDTWTPLNCAGEVE
                     TTSQDNWFGGVKVDPNNANHVYWIGFICSRSLDGGNTWDLIMEDAHVDCHAMYLHPTD
                     NNFRIMGSDGGLFFTYDDFDDYTMDQNIPVTQLYTLDVFPSDTSLLIGGAQDNGSFIR
                     DPSNYWAFANWGDGVSTKFVPVDDNSYYANYQYGGYFGYVDGNVMDLAGLGLGSDRFN
                     WRSPLELNPLNPKTIYFGGSKIYRSNNYGNTMTTISSDLSNGSQGTGLTFGTVFTIHN
                     APADTNYIYVGTDDANVWKSDDYGASWEKITDGLPYRYCMSIETDPEDAETVYVAFSG
                     FRWAESIAHVYKSTDAGETWAPIDGDMPDIPVNDIQIFKTNDTTGLILATDVGVYYSY
                     NDGVNWNALGTAMPIVSVYEINYNEATNYLFAGTYGRGAWKMAMPRKTQNPIAINTLN
                     IADYGMFPNPTSDQLWITLNATHQNVQTTVMALDGKVVKQQLFRTTNQPQISMRDLAP
                     GTYLVKVQVGEQFVVKKVVKS"
     gene            2536..3081
                     /gene="pyrR"
                     /locus_tag="PLB46_02555"
     CDS             2536..3081
                     /gene="pyrR"
                     /locus_tag="PLB46_02555"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRF75424.1"
                     /translation="MQSRIILDSLKFQLTIERLCHQLIENHADFSNACLIGVQPRGVL
                     LAKRILNKLEELLPGVKIKFGVLDATFYRDDFRHHEKPLLAEETDIDFVIKGKNVILV
                     DDVFYTGRTIRSALDALLDFGRPEKIELLVLIDRRLSRHVPITPDYVGKTVDAVTSEK
                     VKVEWSENGTEDKVWIIPYKE"
     gene            3083..4033
                     /locus_tag="PLB46_02560"
     CDS             3083..4033
                     /locus_tag="PLB46_02560"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRF75425.1"
                     /translation="MSNLSVNHLIAIKDLQPEDIHLILNTADNFKEVLSRPIKKVPTL
                     RDITIANIFFENSTRTRISFELAEKRLSADTINFAASSSSVKKGETLIDTVNNILAMK
                     VDMVVMRHQSPGAPVLLSKHIKANIVNAGDGTHEHPTQALLDAYSIMEKLGEKQGKRL
                     SDLKGVKVAIIGDILHSRVAGSNIHALKMLGADVTLCGPPTLMPKHIESMGVRVSYNL
                     KETLEWCDVANVLRIQLERQDIKFFPSLREYSLYYGINRELLDSLSKEIVIMHPGPIN
                     RGVELTSDVADSKQSIILQQVENGVAVRMAVLYLLAGRLD"
     gene            complement(4041..5558)
                     /locus_tag="PLB46_02565"
     CDS             complement(4041..5558)
                     /locus_tag="PLB46_02565"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75426.1"
                     /translation="MKHFLTVVLFTFSQVVYTQYCEIDSTFDYDGWKITSVTPSEDYI
                     EGLIPLSDGKLIAVGPVGSLLHFDIGMVKYNEDGSLDETFGTDGIFLDAPETASLVTY
                     DARIQDDTHFIVGGSWYEDGNKDIFISRFDFFGIRDVSFGIDGYVHLDLGDTDEKISS
                     LALQDDGKIYAAGRTFTGANSDWFVARFNINGTLDTIFGNNGIQLIDITETGYNLEKI
                     LVQADGKIVCCGNTPNMVTGMNIVCARLTNSGILDTTFNHSGIFEYDLDSTYDLALSM
                     AIQNDGKLIIAGISESYGLLLRMLAGGVIDSSFGNNGAFLLPNASFASILLDEFDQII
                     AIGNSHEVAADVDFCIYRIKPNGTLDSTFGNFGRIASDLGQTVEVFVCAAWLYDGKIV
                     SGGYSRGINSNFAVGRYIYGEKVTVPIDYTITKQLHIYPNPTNGSIKIQGELRSGCTV
                     NIINLAGKIITTISNTVDAPEIDYTFDKSIPNGIYFVEIIDNKSKILSSAFILSR"
     gene            5934..7277
                     /locus_tag="PLB46_02570"
     CDS             5934..7277
                     /locus_tag="PLB46_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75427.1"
                     /translation="MRKIISTLIVFVFIFTTALVAQVPLNTFVGPTSMPSDDDPICTV
                     PIYPDVTTNLEGPLEGELVHDFKLYTLEGDSVQLSGVLNDGKPILLIGCNYTCYVFRG
                     KIDVINEMQAMYGDLINIFLMYTVEAHPVIDFSPYFGYENVGADNYAEGILYRQPLTY
                     GERKLIVSDMLDNEEINVPVLIDGPCNDVWMNYGTAPNPAWLIAPDGIVYDAQKWFNK
                     EPDNMYASIDELLGIVGTGTYTPEGTFEAVDEGEEIYYGTVDNMIAAHITFTNTSDDD
                     VLIDFVRESEDIPAGWYTSLCTELCYSPSVDSTTVYLTPGQSEEVRIDFFTDGTPAEG
                     SVTLSCTNHYDATNNYTFTINASSIAATNIENNQIHFTTLFPNPANAGSVIHFDNMQF
                     ADALPVLFGLDGKPIANLKRVNSGDYILPEVATGLYYIQIIAENNIYLSSVTITN"
     gene            7347..8894
                     /locus_tag="PLB46_02575"
     CDS             7347..8894
                     /locus_tag="PLB46_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRF75428.1"
                     /translation="MKKQFLLLLLPISFILFFSCETELPNHLTFDSYVYATTDETGGD
                     WEPVYLTGTGLVVIPEPEDITAESYLDELATVKSFNQSLTNEEQAAVNYWGNNSIIRW
                     FEITEELIAKYNLAPSPDENGEYGVPDPVFPETYPTFPFAHPPYASRAYAYLGASFYD
                     ALITAWHYKYIYNRPAPYKVDPTIVPAYPDNNLPSYPSEDAVIASVAEELLIFLFPLE
                     INYIQEQAQELRDTRIWSGMNVASDIVAGDSIGKTIAEIFIGRAKTDSMKFAQVEQDE
                     YDEMETIASALWGSNWPRWENLEVPQRPIGITPKFGHVVPWWIPSVEAVRPGPPPAIG
                     SDEFNSAKNELLDYTDNLTREQEQIAYFWGDGFGTYTPAGHWNTIAKEYIVQYRFNPL
                     RTARTYAYLNTAMMDAGISCWDTKYFYMYPRPPQADKNIETLFGLPNFPSYTSGHSTF
                     SAAAAEVLGYIFPNEQSLFEQYAKDASESRIYARIHFRFDCETGLTVGENIGNYAVTA
                     AQADGAD"
     gene            complement(8895..9482)
                     /locus_tag="PLB46_02580"
     CDS             complement(8895..9482)
                     /locus_tag="PLB46_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRF75429.1"
                     /translation="MAHIELGNNLQGIRGLLKYNPVTAYPLLLLAESLLQGNSTLSKG
                     ERELIATYTSYKNDCQFCQLSHGGAAATHLGINLDSITAIKSNPDNSDLITPKMKTLL
                     HIAALVQQGGKFVTDDAIQSAKNAGATDAEIHDTVLIAAAFCLFNRYVDGLGTNSESN
                     PESYLEASARMANEGYLRKENLAMMGINQDKSNSL"
     gene            complement(9687..10154)
                     /locus_tag="PLB46_02585"
     CDS             complement(9687..10154)
                     /locus_tag="PLB46_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRF75430.1"
                     /translation="MSNPQQILDHVKQFIQSVGVDPATCWNEQNKAYYIYKGSAKLEI
                     FVSSHPQNDGTTRHYLRIFSGLMKVPATNKEYFYRRCMEISDQSLGVKLTVVPNAAPD
                     NDWVYATYERDINGMDYNETITCVNDMGLWADWLDDQLKTEFGGGAGPAGPAQ"
     gene            complement(10329..10724)
                     /locus_tag="PLB46_02590"
     CDS             complement(10329..10724)
                     /locus_tag="PLB46_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75431.1"
                     /translation="MRKKNWIKYGIGIFFAGAAMIALMGLATMWLWNWLVPELFSGSS
                     ITFFQAIGLIALGKLLTGFMGMGRHGWGKHSNGWNHKKEMYWKQKMEAKMSSMTEEER
                     EKFKRYYYDRCGWKTKRDEAVEDKTQTQQ"
     gene            complement(10737..11333)
                     /locus_tag="PLB46_02595"
     CDS             complement(10737..11333)
                     /locus_tag="PLB46_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF75432.1"
                     /translation="MDESLHLGTFMTQQQNEIIEQAVKNERKKLLKFIKTRVGNEEDA
                     SDILQDVFYQLASNHGMVETIENMASWLYRVTRNKIIDWYRKRKTESIDTMTAFDDED
                     EDGYFSNLAALSSAQSDNPDAVFERQLVWDTMYEALNELPEEQREVFILHELENKSFN
                     EIAEQTGVSVNTLLSRKRYAVLYLREKLKDLYDELINK"
     gene            complement(11520..12329)
                     /locus_tag="PLB46_02600"
     CDS             complement(11520..12329)
                     /locus_tag="PLB46_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF75433.1"
                     /translation="MKKINLLLFSLVILTLAISGCKGGKKDKEQTELTDLPEMETVAV
                     SGMKVDTLEMPSTDGLTMRAVVYTIDSTKPVIVLCHQARMNNYEYAEIAPKLCSMGYN
                     CVALDQRSGGAMETHENITNKNALAKNMATGYTDALPDITAGVNYAATTFGQQVILWG
                     SSYSASLVLKVAMDNPNVKSVITFSPILKFDDGSTAADYFKGYNGKPIFMTSTEKEAA
                     PLTKAMSHLGDDVLTQYYPDMKGTHGSKALWSSDPSSADYWEAVTKWLAAH"
     gene            12475..>13875
                     /locus_tag="PLB46_02605"
     CDS             12475..>13875
                     /locus_tag="PLB46_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75434.1"
                     /translation="MNFIKSKSLLLWLTALFFVLIAQSCRKDSFITTPDAKLNFSEDT
                     VHFDTVFSTVGSITLPLKLFNNYNENLNISSIQLAGGAFSQFRMNVDGESGSQFTDIE
                     ILPNDSIYIFIEVTVDPNDDALPFVIEDSIIFTTNGNIQDVKLIAYGQNANFHYGEIL
                     CDTTWTDNLPHVIIGSVLVDTLCSLTITEGTDIYLHGGSSFYVLGTLNVLGTKDSLVT
                     FQGDRLEDFYKDVPGQWEGIYLLRGSFDNLIQYAEIKNANKGISLGYSTDTGLATFLE
                     LQPTLTLENTKIFDIQYYGIESLNSKIKATNVLIYNVGQSNTALTLGGDYEFRHCTMA
                     NYSSRYLTHQAPVLLIWDFYPFGPELNLQNDLTKADFINCIIYGSIAEGNELSIDTLT
                     LPTVFNFNFDHCVIKSAINQDLLNDVGCFFNTDPVFANIGERNYCPTTGSPALNAAID
                     IPSDPVLIDIIGNPRPF"
BASE COUNT         4242 a         2539 c         2638 g         4456 t
ORIGIN      
        1 gaccgcaatt ttgaaagtgc tgaaaacgaa gaagaagaaa ctgcggttga tgaatggtta
       61 tatgcccaaa aggctttccc gtatggtgtt gcagacaaac atgctcagcg aaatgccata
      121 gctcatatgc aaagtgcaag agctcaaaat gccttgttta aaactactgc tgatggggac
      181 gattggtcgt ttatcgggcc atataatatt ggtggccgca ttctggatgt agattgtccc
      241 ggcggaaata aaaacattat ttatgtagct acagcttcag gtggggtatt aaaaacctat
      301 aatggtggtg atacctggca aaatattttt gatactgaat ctactttgtc aataggtgac
      361 atcgcaattg atcctgttga tacagcaaca atttatatcg gtaccggtga accgggaaat
      421 ggtgttggct ctgtaactta tgatggcaat ggtatttata aatctaccaa ctctggtgcc
      481 acatggacta atatcggctt aagtgaaggt ggaaatacag gcagaattgc tattaatcct
      541 gataatacac aaattatttt tgcggctatg ttgggtgatt tattccaaaa tactcctgac
      601 cgcggaattt atcgtaccga aaatggtgga acaacctggg aaaaagtact gtatgtaaat
      661 gattcaacag gtggaattga tgtggctatt aatccggaaa atccaaatat tatttatgca
      721 tctacttggg aacgtgtgcg cagatataac agaagagatt atgctggtgt aggctcacga
      781 atttatcgca gtgaagatgg tggcgataca tgggaaatgc tttcaacagg tttgcctttt
      841 ggttcaacag aattatcacg catcatgtta tcgattgcac cttccgaccc ggatattgta
      901 tatgcttcaa ttgtagcaag taacgataaa ttaaaagata tttataaatc taccaatggt
      961 ggtgatacat ggactccgtt aaattgtgct ggtgaggttg aaactacttc tcaggataat
     1021 tggtttggtg gagttaaagt tgacccgaat aatgccaatc atgtttattg gattggattt
     1081 atttgctcac gcagcttaga tggcggtaat acctgggatt taataatgga agatgcacac
     1141 gtggattgtc acgccatgta tttacatcct acagataata attttcgcat tatgggaagt
     1201 gatggcggat tatttttcac ttacgatgat tttgatgatt ataccatgga tcaaaatatt
     1261 cctgttacac aattatatac attagatgta tttcctagtg atacatcatt attaattggt
     1321 ggcgcacaag ataatggtag ttttattcgc gacccaagta attattgggc tttcgcaaat
     1381 tggggcgatg gtgttagtac gaaatttgtt ccggttgatg ataattctta ttacgctaat
     1441 tatcagtatg gtggttattt tggttatgta gatggaaatg taatggattt agctggattg
     1501 ggtttaggtt cggaccgatt taattggaga agtcctttag aattaaatcc gttaaatcca
     1561 aaaacaattt attttggtgg aagtaaaatt tatcgctcaa ataattatgg aaataccatg
     1621 acaaccatca gcagcgattt gtctaatggc agtcagggaa caggacttac tttcggaacc
     1681 gtatttacca ttcataatgc acctgcagat accaattata tttatgttgg aacagatgac
     1741 gctaatgttt ggaaaagtga tgattatgga gccagctggg aaaaaattac agatggctta
     1801 ccttatcgct attgtatgag tattgaaacc gacccggaag atgcagaaac cgtgtatgtt
     1861 gcatttagcg gcttccgatg ggctgaaagt attgcgcatg tttacaaaag taccgatgca
     1921 ggtgaaacat gggcaccaat tgatggcgat atgccggata ttccggttaa tgatattcag
     1981 atatttaaaa ccaacgatac cacaggatta attttagcaa cagatgtggg tgtttattat
     2041 agttataacg atggtgtaaa ttggaatgca ttgggaacag caatgccaat agtatcggtg
     2101 tatgaaataa attataacga agccacaaat tatttgtttg ccggtaccta cggaagagga
     2161 gcctggaaaa tggctatgcc aagaaaaacg caaaatccaa ttgctataaa cacattaaat
     2221 attgcagatt atggtatgtt tccaaatccg acttccgatc agctatggat tactttaaat
     2281 gctacgcatc aaaatgtgca aacaacagtt atggcgttag atggtaaggt ggtgaaacaa
     2341 caattattcc gtacaaccaa tcagccgcaa atcagtatgc gcgacctggc acccggcact
     2401 tatttggtaa aagtgcaggt aggagagcag tttgtggtga aaaaggttgt gaaatcataa
     2461 aatttaccct tacagatagt tgccaacagg aatacatttt gctgtttggc tactatctgt
     2521 taactttgtg ttttaatgca aagccgcatt atactcgaca gtttaaaatt ccagctcacc
     2581 attgagcggc tttgtcatca attaatcgaa aaccatgctg atttttccaa cgcctgtctc
     2641 ataggagtgc agccacgtgg cgttttgctg gctaaaagga tattaaacaa gctggaagaa
     2701 ttattaccgg gtgtcaaaat aaaattcggc gttttggatg ccacttttta ccgcgacgat
     2761 ttccgtcatc acgaaaaacc attacttgca gaagaaacag atatcgattt tgtgataaaa
     2821 ggcaaaaatg tgattctggt agatgatgtt ttttacacag gcagaaccat acgaagcgca
     2881 cttgatgcat tattagattt tggccgccct gaaaaaattg aattattagt attaatcgac
     2941 cgacgtttga gtcgccacgt accaattaca cccgattatg tcggcaaaac cgttgatgct
     3001 gttaccagcg aaaaggtgaa agtagaatgg agcgaaaacg gtacggaaga taaagtttgg
     3061 attatacctt ataaagaata atatgagcaa cctgagtgtc aatcatctga tcgcaatcaa
     3121 agaccttcaa cctgaagaca ttcatctcat tttaaatacc gccgataatt tcaaagaagt
     3181 attaagcagg ccaattaaaa aagtgccaac actgcgcgat attactattg ccaatatatt
     3241 tttcgaaaat agtacacgca cacgaatttc atttgaatta gcagaaaaga gattaagtgc
     3301 cgataccatc aattttgctg catcaagttc ttctgttaaa aaaggagaaa ccttaattga
     3361 taccgtaaat aatattctcg ccatgaaagt agacatggtg gtaatgcgtc atcaaagtcc
     3421 aggtgctcct gtattattga gcaaacatat caaagccaat attgtaaatg ccggtgatgg
     3481 tacgcatgaa catccaactc aggcattact tgatgcctat agtatcatgg aaaagttagg
     3541 cgaaaaacaa ggcaaacgat taagcgattt aaaaggagtg aaggtggcca ttattggaga
     3601 tattttacac tcacgcgtag caggttctaa tattcatgca ttaaaaatgt taggtgctga
     3661 tgttacctta tgcggacctc caaccttaat gcctaaacac atcgaatcga tgggcgtgag
     3721 ggtatcgtat aatttaaaag aaacattaga gtggtgtgat gttgccaacg tgttgcgcat
     3781 ccaactcgaa cgtcaggata ttaaattttt cccttcatta cgcgaatatt cattgtatta
     3841 cggtataaat cgcgaattat tagattccct cagcaaagaa attgtcatca tgcacccggg
     3901 cccaatcaac cgtggtgtag aactcaccag cgacgtagca gacagcaaac aatccattat
     3961 tttacaacag gtagaaaatg gtgttgctgt gcgtatggct gtcttgtatt tattagctgg
     4021 gagattggat taaattaaaa ttaacgactg agaataaatg cagatgacaa tattttactt
     4081 ttgttatcaa tgatttctac aaaatatatt ccattcggta ttgatttatc aaaagtgtaa
     4141 tctatttcag gtgcatctac agtattgcta atggttgtaa taattttacc ggctaagtta
     4201 atgatgttaa ctgtgcaacc ggatcttaat tctccctgaa tttttatact accatttgtt
     4261 ggattgggat aaatatgtag ttgctttgtt atcgtataat ctataggtac ggttaccttt
     4321 tcgccataaa tatatcgccc cactgcaaag ttagaattta taccacgaga ataacctccg
     4381 ctaactattt tgccatcata tagccatgcg gcacatacaa aaacttcgac ggtttgtcca
     4441 aggtcactag caatcctgcc aaaattacca aatgtactat ctaaggtgcc attcggtttt
     4501 attctataaa tacagaagtc gacatccgct gcaacttcat gtgaattacc aatcgcaatt
     4561 atttgatcaa attcatccaa caaaatgcta gcgaaacttg cgtttggcag aaggaatgcg
     4621 ccattgtttc caaatgatga atctattaca ccacccgcta acatacgaag tagtaaaccg
     4681 tatgattcag atatgccagc aataattaat ttgccatcgt tttgaatagc catactcaaa
     4741 gcaagatcgt atgtactgtc caaatcatac tcgaatattc cactatggtt aaatgttgta
     4801 tctaatattc cggaatttgt taatctagca cagacaatat tcattcctgt aaccatgttt
     4861 ggtgtatttc cacaacaaac tattttacca tcagcttgaa caagaatttt ttctaaatta
     4921 taaccagttt ctgtaatatc aattagttga ataccattat tgccgaaaat agtatcaagt
     4981 gttccattta tattaaagcg agcaacaaac caatcggaat ttgcaccagt aaatgttcgc
     5041 ccagctgcat atattttccc gtcatcctgc aatgcaaggg aacttatttt ttcatcggta
     5101 tcccccaaat ccagatgaac atatccatca atcccaaaac ttacatctct tattccaaaa
     5161 aaatcaaacc tcgaaataaa aatatctttg ttaccatcct cgtaccaaga acctccaaca
     5221 ataaaatgtg tgtcatcctg tattctggca tcataggtaa ctaagctagc agtttcggga
     5281 gcatctagaa atatgccatc agttccaaat gtttcatcca gactgccgtc ctcattatac
     5341 ttaaccatgc caatatcgaa gtgaagcaaa ctgccaacag gtcctacagc tattagtttt
     5401 ccatctgaaa gtggaattaa gccctcaata taatcttctg atggagtaac ggatgttatt
     5461 ttccatccat cataatcaaa agtgctatca atttcacaat attgagtata tacaacttga
     5521 gaaaatgtaa atagtacaac ggtaagaaag tgtttcatgt gaacatacaa ttattttgct
     5581 ggggcataat acagtaaaag taataaaaag taataaaatg atgtgcaaga aatgtgtttt
     5641 tttttgaata gcaggttggg tttccaatta ccacaaattt cctgtgtaaa ttatttgata
     5701 agtttgtaaa agaaaggtaa aacttgtttc aagtaagttt aaatgcaatt gaatattaat
     5761 aattccgacc ttaaattcag catatattta gtctgtaaac accaccctta ttgactttca
     5821 atgaatttca gttgattttt ttgtaagcaa tttatccgtt tacatttcat taacgcaatt
     5881 tggtgaattc actttgtcgc agcttctttg tgccggctca aagaaaaacc cttatgcgaa
     5941 aaattatatc tacccttatt gtatttgttt ttatattcac tacagcttta gttgctcagg
     6001 ttccgttaaa cacttttgtg ggtccaacca gcatgccttc agatgatgac ccgatttgta
     6061 ctgttccaat ttatcctgat gtaaccacca atctggaagg gccattggaa ggtgaattag
     6121 tacacgattt taaattatat actttagaag gtgactccgt tcaattaagc ggtgtattaa
     6181 atgatggtaa accgatttta ttaattggtt gtaattacac ttgttatgtg tttcgcggca
     6241 aaattgatgt gattaatgaa atgcaggcta tgtatggcga tttaattaat atctttttaa
     6301 tgtataccgt tgaagcacat cctgtaattg atttcagtcc gtattttgga tatgaaaatg
     6361 tgggtgcaga taattatgca gaaggaattt tatatcgcca acccttaact tatggtgaac
     6421 gcaaattaat tgtgagcgat atgcttgaca atgaagaaat taatgttcct gttttaattg
     6481 acggaccttg caacgatgtt tggatgaatt atggcactgc gccaaatcct gcttggttaa
     6541 ttgcacccga cggtattgtt tatgatgcac aaaaatggtt taataaagaa ccggataata
     6601 tgtacgcatc aatcgatgag ttattaggta tagttggaac aggcacttac acaccggaag
     6661 gaacatttga agcagtagac gaaggtgaag aaatttatta tggtactgta gataatatga
     6721 ttgcggcaca tatcaccttt accaatacca gtgatgatga tgtattgata gattttgttc
     6781 gcgaaagtga agatattcct gcgggatggt atacttcttt atgcaccgaa ttatgttatt
     6841 ccccaagcgt tgattctaca actgtatatt taacaccggg acaatcggaa gaagtgcgta
     6901 tcgatttttt cacagatgga acacctgctg aaggtagtgt tacattatcc tgcaccaatc
     6961 attacgacgc gactaataat tatacattta ccataaatgc ttccagtatt gctgcaacca
     7021 atattgaaaa taatcaaatt cattttacaa cattatttcc aaatcctgca aatgccggaa
     7081 gtgtaattca ttttgataat atgcaatttg ctgatgcgtt acctgtttta tttggattag
     7141 acggtaaacc aattgccaac cttaaacgag taaattcagg agattatatt ttgccggaag
     7201 tagcaaccgg attatattat attcaaataa ttgctgagaa taatatttac ctgtcttcag
     7261 ttacaattac gaactgaggt taggtttggt ttaaactggc ggaagttgag tggctatctc
     7321 atacttccgc ttttatcatt atagttatga aaaaacaatt tttattgctg ttattaccga
     7381 ttagtttcat actatttttt agttgtgaaa cagaattacc taatcatcta actttcgata
     7441 gttatgttta tgcaactact gatgaaacag gtggcgattg ggagcctgta tatttaaccg
     7501 gtaccggatt agtggtaatt cctgaacctg aagatattac tgctgaaagt tatttggatg
     7561 aattagctac agtaaaatct tttaatcagt cactcactaa tgaagaacaa gctgcagtaa
     7621 attattgggg aaataattcc atcattcgtt ggtttgaaat aacggaagaa ttaattgcga
     7681 aatataatct cgccccatca ccagatgaaa atggtgagta tggtgttccg gatcctgtgt
     7741 ttcccgaaac atatccaact tttccatttg cacatccacc ttatgctagt cgcgcatatg
     7801 cttatttagg tgcatccttt tatgatgcgc taattaccgc ctggcattat aagtatattt
     7861 acaatcgtcc ggcaccatat aaagttgacc ctacaattgt tcctgcttat cccgataata
     7921 atttaccatc gtatccaagt gaagatgccg ttattgcatc agttgcagaa gaattactta
     7981 tctttttatt tccattagaa attaattata tacaagagca ggctcaggaa ttacgtgata
     8041 cacgcatttg gtcaggaatg aatgtggcaa gcgatattgt tgcaggagat tcaattggta
     8101 aaactattgc agaaatattt atcggaagag caaaaaccga tagtatgaaa tttgcacagg
     8161 tggaacagga tgaatatgat gaaatggaaa ctatcgcctc ggcattatgg ggaagtaact
     8221 ggccaagatg ggaaaacctc gaagtgcctc aacgcccaat tggtatcaca cccaaatttg
     8281 gacatgtagt gccctggtgg ataccatcgg ttgaagctgt gcgacccgga ccaccacctg
     8341 caattggttc ggatgaattt aattcagcaa aaaatgaatt attagattat acagataatt
     8401 taacacgtga acaagaacaa attgcctatt tctggggcga tggattcggt acctacacac
     8461 cggcaggcca ctggaataca attgccaaag aatacatcgt tcaatatcgt tttaatccat
     8521 tgcgaacagc acgcacatac gcttatttaa atacggctat gatggatgcc ggtatcagtt
     8581 gttgggatac aaaatatttt tacatgtatc ctcgaccgcc tcaagctgat aaaaatatcg
     8641 aaacactttt tgggttgccc aattttccat cgtacacttc aggacatagt actttttccg
     8701 cagctgcggc tgaagtactg ggatatattt ttccaaatga acaatcttta tttgaacagt
     8761 atgcaaaaga tgcttctgaa tcacgcattt atgcacgaat tcactttcgt ttcgattgtg
     8821 aaacgggatt aactgtagga gaaaatattg gtaactatgc agtcactgcc gctcaagcgg
     8881 atggagcaga ttaattacaa agaattagat ttatcctgat tgatacccat catggctaaa
     8941 ttttctttgc gtaaataacc ttcatttgcc atacgtgcgg aggcttccaa ataagattct
     9001 ggattacttt ctgaattagt ccccaatccg tcaacatagc ggttaaataa acaaaacgct
     9061 gctgcaatta atacggtatc gtgaatttct gcatctgtag cacctgcatt ttttgcagat
     9121 tgaattgcat catcggtgac aaattttcct ccttgttgca caagcgctgc aatatgcaat
     9181 aatgttttca ttttaggtgt tattaaatcc gaattatcag ggttcgattt tattgctgtt
     9241 attgaatcta aatttattcc caaatgcgtt gcagctgcgc caccgtgtga taactgacaa
     9301 aactgacaat catttttata cgaagtatag gtggcaatta attcacgttc acctttgctt
     9361 aaggttgaat ttccctgtaa taaagattca gctaaaagca agaggggata agctgttaca
     9421 ggattatatt ttaacaaccc tctgattccc tgcaggttat tgcctagttc gatatgtgcc
     9481 ataatttttt attgtaaatg taaaacacaa atattttaat tgcatttaat ttacaaaata
     9541 ttagcaattt gctgtggtta tatttttagt attttttttt aagtaaaatt tgttttctat
     9601 acgctctaat taatttaaaa atgaggatgc ttaaaataaa aaatctccgc aaataattgc
     9661 agagattttt atcagtttga aattttttat tgcgcaggac cagcaggacc cgcaccaccg
     9721 ccgaattctg tttttaactg gtcgtctaac cagtctgccc ataaacccat atcattcacg
     9781 caagtaatgg tctcattata atccatgcca ttaatatcgc gttcataagt tgcgtaaacc
     9841 caatcgttat caggtgcagc atttggcacg acagttaatt taacgccaag gctctggtcg
     9901 cttatttcca tacaacgacg atagaaatat tccttattgg ttgcaggcac tttcattaaa
     9961 ccgctgaata tgcgcaggta atgacgagtg gttccgtcat tttgtggatg actggaaaca
    10021 aatatttcta atttagctga gcctttatag atataatagg ctttattttg ttcgttccag
    10081 catgttgccg ggtcaacacc aacgctttgg ataaattgtt ttacgtggtc gagaatttgt
    10141 tgtgggttac tcataattat tagtggtttt ttataaatta agacactaat gtaggatttt
    10201 tatcgtaacc acaaaaactt ttatattaaa ttaacggtaa accgtgaatg ataaattgca
    10261 gtatctataa aatacaaaac cgccgcaaat tcgcatttac gacggtttta tattaagttt
    10321 ttgaaaactt attgctgagt ttgagttttg tcttccactg cttcatcgcg tttggttttc
    10381 catccacagc ggtcgtaata ataccttttg aatttttcgc gttcttcttc ggtcatactc
    10441 gacattttgg cttccatttt ttgtttccaa tacatttctt ttttatggtt ccagccattg
    10501 ctgtgttttc cccatccatg gcgacccatg cccataaatc cggtaagtaa tttacctaaa
    10561 gcaattaatc caattgcctg aaaaaatgtg atgctgctgc cactaaataa ttcgggtacc
    10621 agccaattcc acaaccacat ggtggctaaa cccataagtg cgatcattgc tgcaccggca
    10681 aaaaatattc cgataccata tttgatccaa ttcttttttc gcatatcaat aatgttttac
    10741 ttgttaatta attcgtcgta taaatctttt agtttttctc taagatataa tacggcatat
    10801 ctttttcgtg ataataaggt attaactgaa acaccggttt gttctgcaat ttcattaaag
    10861 cttttatttt cgagctcatg caaaataaaa acttcacgtt gctcttccgg taattcattt
    10921 aatgcttcat acatggtatc ccaaactagc tgtcgctcaa acacagcatc cggattatcg
    10981 gattgtgctg atgataaagc tgctaaattg gaaaagtatc catcttcatc ttcgtcgtca
    11041 aaagctgtca tggtatcaat cgattcggtt tttcgcttgc gataccaatc gataatttta
    11101 tttcttgtta ccctgtacag ccatgaagcc atattttcga tggtttccac catgccatgg
    11161 ttggaggcta attgataaaa tacatcctgc agaatgtcac ttgcgtcttc ctcattgccc
    11221 acacgtgttt taatgaactt caatagcttt ttgcgttcat ttttaaccgc ctgctcaata
    11281 atctcgtttt gttgttgagt catgaaagtg cctaaatgta aactctcgtc catacttgaa
    11341 atattgacga aagtaggtgc gaagatattt taaattatgc tgtgttttag attgaccttt
    11401 tgagctattt cggctgaaaa gctttgtttt cgagcatttc agcagaatat gctcagccat
    11461 aaaaaaactc agtatagttg taaaaccata ctgagttttc ctgttgagtt agttgatttt
    11521 tagtgcgcag ctaaccattt agttacggct tcccaataat ccgcagatga tgggtcgcta
    11581 ctccatagcg ctttagagcc atgtgtacct ttcatatcag gataatattg cgttaataca
    11641 tcatcaccta aatgcgacat cgccttagta agcggtgcag cttctttttc agtagatgtc
    11701 ataaatattg gcttgccatt atatccttta aagtaatcgg ctgcagttga accatcgtca
    11761 aattttaaaa tcggactgaa agtaattacc gatttaacat tcggattgtc catggccact
    11821 tttagtacca aactggcaga ataactactt ccccacaaaa taacctgctg cccaaatgtt
    11881 gttgcggcat aattaacgcc tgccgtaata tccggcaatg catcggtata accggttgcc
    11941 atgttttttg ccaacgcatt tttattggta atgttttcat gtgtttccat tgcaccaccg
    12001 gaacgctgat ccaatgctac acagttataa cccattgaac ataatttagg cgctatttca
    12061 gcatattcgt aattattcat tcttgcctga tgacataata ctattaccgg ttttgtgcta
    12121 tcgatggtat aaacaacggc acgcatggta agtccatcgg tggaaggcat ttcaagcgta
    12181 tcaactttca ttccgctcac cgcaactgtt tccatttccg gtaaatcggt aagttcggtt
    12241 tgttctttgt ccttttttcc acctttacag cctgaaatag ccagtgtaag gatgactaaa
    12301 ctaaagagta atagattaat ttttttcatt ttgctttgag ttttgtggtg caaaagtaca
    12361 tcaagcgaca cgggtaacac aattttaaca ttacaagggt ttaaaagttt tgctaatttt
    12421 gacacactat tgtgttgtta gattacgtta aagagccaaa gctgctgtat ccaaatgaat
    12481 ttcatcaaaa gtaagtcctt attgctgtgg ttaacagcac ttttttttgt gttgattgct
    12541 cagagttgcc ggaaagattc cttcattaca accccagatg ccaaactcaa ttttagcgag
    12601 gataccgttc attttgatac cgttttttca acagtaggtt caattacgct tccactaaaa
    12661 ttattcaata attataatga aaatctcaat atcagtagca ttcagcttgc cggtggagcg
    12721 ttttcgcagt ttagaatgaa tgtggatggt gagtcaggtt ctcagtttac cgatattgaa
    12781 attctcccca acgacagcat ttatatattt atagaagtta ctgtagaccc taatgatgat
    12841 gccctgcctt tcgttattga ggattctatc atttttacta ccaatggcaa tattcaggat
    12901 gttaaactga ttgcttatgg tcaaaatgcc aatttccatt atggggagat tttgtgtgac
    12961 accacctgga cagataacct gccacatgta attatcggtt cggttttggt tgatacttta
    13021 tgctctttaa ctattaccga aggtacagac atctatttgc acggcggaag ttccttttat
    13081 gttttaggaa ctttgaatgt tttaggcacc aaagacagtc tggtgacctt tcagggagat
    13141 agattagaag atttttataa agatgttccg gggcaatggg aaggcatcta tttgttgcgg
    13201 ggaagttttg ataatcttat tcaatatgca gaaatcaaaa atgcgaataa ggggatttca
    13261 ttagggtatt cgactgacac cggtttagct acatttttgg aattgcaacc gacattaaca
    13321 ctggaaaata cgaagatatt cgacattcaa tattatggta ttgagtcgct caatagtaaa
    13381 attaaggcga ctaatgtgct tatttacaat gtggggcaat ccaatacagc tcttacgctt
    13441 ggaggcgatt atgagtttcg tcattgcacc atggccaatt acagttcaag gtatttaacc
    13501 caccaggcac cggtgctttt aatttgggat ttttatccat ttggaccgga attaaactta
    13561 caaaacgacc tcacgaaagc ggatttcatc aattgtatta tttatggcag catagccgaa
    13621 gggaatgaat tatcaattga tacattgacg ttaccaactg tctttaattt taacttcgac
    13681 cattgcgtta taaaatcggc tataaatcag gatttgctta acgatgttgg gtgtttcttt
    13741 aataccgacc cggtttttgc caatattggg gaacgtaatt attgcccgac tacgggttcg
    13801 ccagcgctta acgctgctat tgatattccg tcagacccgg ttttgattga cataataggc
    13861 aatccgaggc ctttt
//
LOCUS       DAORKW010000032        13807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_61_length_13807_cov_4.833333, whole genome shotgun sequence.
ACCESSION   DAORKW010000032 DAORKW010000000
VERSION     DAORKW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61_length_13807_cov_4.833333"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="PLB46_02610"
     CDS             <1..158
                     /locus_tag="PLB46_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HRF75435.1"
                     /translation="PQSQTLKFDPELKYISHWVPELNELSYPKPIVDHSFARDRVLKV
                     YKAALSR"
     gene            complement(134..919)
                     /locus_tag="PLB46_02615"
     CDS             complement(134..919)
                     /locus_tag="PLB46_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75436.1"
                     /translation="MKCTLVLFSLATLFLCACNKSKPDSSTEYQLQIANSITKSMIEL
                     RLNSLIKMKSDDARIEKLIQPEITIDSLIEDYIDGLGKNNSNSNYNSLMLFKRKIINV
                     YADGYGRSELIDSNITYYNNLDTFFTAAEVTNQLLNLELQVVTNKHRRITNCGWDYID
                     QAIDIGDKKYLCIIQNNIDFREAKKLPLYVEVSVNNHYLPNYAYKVEKMHHMFVVQIN
                     LEEYPIAPLDNIRVFLVINPENEHKEIFLKYFTLAPYLDNAAL"
     gene            1178..2545
                     /locus_tag="PLB46_02620"
     CDS             1178..2545
                     /locus_tag="PLB46_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF75437.1"
                     /translation="MPNWIYYFMPHNVVNKLEKRRLTYALWVISGLGIFFILVLIVHT
                     ACKMTPVHYYADAFGLIGSYLAASFIKKNKLVLAVKTIVITLLVAILVYGPIVDYVEP
                     GPVMFLRLYVTIFALEAALLLMVSFYIAILNYVYLALLFTSYVLLHFFVIVNKNGWET
                     VNPEMISYGVIGVLAINISCFIASLMLRYNNDLMNKINADNTAIVEHNIRLEAEVKNR
                     TSDLQNSNDNLKQFAHIVSHELKEPLRTISGFSGLLQKKLDNLNVDDPDIKDYTNTIQ
                     NSSRHMANLVKEMLTFSSVQQSPKSLVEIKMNELIELVLNQIDNIVQEKKAEITWTAL
                     PNAYGESLLVFQLVQNLIINGIKYQPENNTPKITISGSPKGQFIEYVVTDNGIGMSNE
                     TLLKIFDPLYREHKHTYGDGTGMGLSICKKIVDAHHGKIWVSSQLSAGTCFHFTLPAY
                     NIPIT"
     gene            2636..4303
                     /locus_tag="PLB46_02625"
     CDS             2636..4303
                     /locus_tag="PLB46_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HRF75438.1"
                     /translation="MNRLLITLLLLTGMQLHAQKQIIVDAPVEKATVFLTGAQLFHQA
                     AVNLPKGQSEIILQGLSMSLDPNSVQGGGKGEFTILDIQYRLFYPEPKPNAVVLTETD
                     KKIKSINDSIVELDFDLRTFYNRREVLNVQKQMMLNNTLYKGTGSDSLVLIQQSVEYY
                     DKKLNDIYVELLQVEKKEYQLNLKRAAMVARVYELQNYRNQQAALEQPNNPIPQIIMS
                     VQTDYALSATVEANYITYNAGWYATYDIRATDIAKPVDIAYKANVWQNSGIDWKDVKL
                     TCSTGNPMIGNNLPEITTWYLGYYDYYYNRDEAKTITLGSVATEDMDDAKVLSDKYLN
                     APSAVDAGYANNYTTPVQTIANVEFDIQLKYSIPNDGKGHIVALQTKQLPTTYNYLIV
                     PKVEQAAFLIARITDWESLNLLPGNANIYFNNTYVGKTNINPLALADTLSLSLGRDRA
                     IEVKRTQLTDKSSERILATNAKKTMAFEIEIRNGKAIPIEVIIKDHIPVSQKESIKVE
                     LFEKDGGELDELTGIITWREKLKTKEKKDFNLEFEITYPKNEPLSLN"
     gene            4701..5336
                     /locus_tag="PLB46_02630"
     CDS             4701..5336
                     /locus_tag="PLB46_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75439.1"
                     /translation="MKILGGLNRIVSLSLLGFLLSLTTASFAGAGVSKSPYHKDHNPL
                     SFYYFGYNTINPGFTLGTHINLSWTKMEKAGCRGSKISDRQFVMIPTIGMFQNEANTL
                     SAYGALEFNFNITFSKGLTMQFFGAPGYAQMLSGDETISGNDNANSRVLSTTAHSGFM
                     PTAGAGLGYNFQKMNGKDCPLAISFRGMATSVDVANLAVFPSFQTGITYSF"
     gene            5406..7808
                     /locus_tag="PLB46_02635"
     CDS             5406..7808
                     /locus_tag="PLB46_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75440.1"
                     /translation="MKKYAFLIVAVLATFTASAQYPLVNIADIQFKDDAALAAEDDLS
                     AYDGDTVRIQGIVTFDPCDYALSSSGSRMGTYLQDPDEDEFAGIHVLIDWPAIGWTDL
                     ESLNDATLFVDNFQVGNIVECTGIVNTFEGNTQFWLIPVESEIIGFGVVPDPDEREIA
                     EFMVSDGAGGQVINTLDGEKYEGKYVEFTNVYVTDVVPSGLRWFWYLQDDAGNKIQIR
                     DMSGHFRNDTYDDECNLWAGGAAGETNTPLTYEPPAIGTNLAYVRGIILEFTAETQYG
                     IAPLLLSDIGPSLASPPVITNITRTPTVATAAETVVVSADITDLDGFVSSANLYYSYG
                     YGSTDWNLATMSNVGADSWEGEIPGPGIAFDSMYVNFYLEAFDDDANVITSPSPASPL
                     TYIVYNDGITSIVQIQNTPFPSGSSVWANDSIPTMNIEAIVTATTKTYDLGMVAVQEG
                     SGQYEGIFIKSVPGDGTDALLRGDKIKITAGKVIEEFGVTKLTNITYTLVSQMNDLPA
                     FVTGINPTDVDAKVYSATEPWEGMLVKFDNAFVTSNNADAAGGGAFGEWRVNTSNTPE
                     VGMRCDDYSYEINFEFGVDTLTMGEELTYIQGLMYYSFSNWKLLPRDRNDIEGYTTVY
                     PNSIVAFNFTSPAATGAIDQAAGTINLTVPMGTDVTALTPSIDFTGQYVDPASGIAQN
                     FTSPVVYTSYSPVTFEPKTYTVTVTQNVGIESFNLSEIQVYPNPTSDVLTVEIGAPTD
                     MQVSIGIKDLEGRNIQEINELCYVGKNIFAFDLRSVANGLYLLQISSKDKTTILKIEV
                     SK"
     gene            complement(8062..8481)
                     /locus_tag="PLB46_02640"
     CDS             complement(8062..8481)
                     /locus_tag="PLB46_02640"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRF75441.1"
                     /translation="MSGNRTLTMIKPDAVRKNSIGGILQMINEAGFRIIAMKYTKLSK
                     EKAGEFYAVHKERPFYGELVDFMSSGPIVAAILEKDNAVEDFRKLIGATDPSKAEEGT
                     IRKKYASSIGENAVHGSDSDENANIESDFHFSKLERF"
     gene            8571..9584
                     /locus_tag="PLB46_02645"
     CDS             8571..9584
                     /locus_tag="PLB46_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRF75442.1"
                     /translation="MKDLGLLFNHLKQPRRIVLLTHVRPDGDAVGSALALKSYFEIKG
                     HTADVIVPDDFPDFLNWMFQSEKAFNHQKNPKTCFAVLARAEVIFFLDFNALKRIENL
                     GEDVKKLNRPYVSVMIDHHREPDYFTDYMLWNDKASSTAELVYDFIHMAGDAELINTE
                     MAACIYAGMALDTGIFQFSNTTSRVHEIAAQLMQKGINIEQIHNNVYNQYGENRMRFV
                     GFLLAEKMVVVAEHRAAYMSITMAEAEKYKLNNGDKEGIVNLPLAMRDVDMAVLFTED
                     RDKIKISFRSKGEVNVEFLARNFFNGGGHRNASGGSSKLSLDETVKRFVEVLQEFAQQ
                     QNN"
     gene            9609..10250
                     /locus_tag="PLB46_02650"
     CDS             9609..10250
                     /locus_tag="PLB46_02650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF75443.1"
                     /translation="MKKTSLFLLMIMFVQALAFGQSDKKKYKHLPDGLYAEMQTSKGN
                     ILLFLEFQKAPLTVANFVGLAEGKIENAAKPLGTPFFDSLIFHRVIADFMIQGGDPKK
                     NGTGGPGYAFKDEFHSDLKHSGPGILSMANSGKFTNGSQFFITHKETPWLDNKHAVFG
                     HVVEGQDVVNAIAQGDLIVHVNIISKGQALKKNDPAAVFKEFETKGDVKRSSK"
     gene            complement(10299..10712)
                     /locus_tag="PLB46_02655"
     CDS             complement(10299..10712)
                     /locus_tag="PLB46_02655"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRF75444.1"
                     /translation="MMGINMRKIFMIVFLIGLTSSCEDIRVDQATDHISRSWSVYKFE
                     IDGYDQTPLFTSTYLNYNITFTDTSDYTETYNLGATPVSITGIYIFSDKASILTLSDE
                     FQSRVFNILTLTNNEMTLEDRNAETNQVYYFEVVL"
     gene            complement(10714..12105)
                     /locus_tag="PLB46_02660"
     CDS             complement(10714..12105)
                     /locus_tag="PLB46_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF75445.1"
                     /translation="MHIEQLYTNCLAEAAYYIESDGVAAVIDPLREPEPYLEKAASRN
                     ATIKYVFETHFHADFISGHLDLAKKSGAAIIYGPEANTAYTVINATDGQEFNIGKIKI
                     RVLHTPGHTLESTCYLLIDEQGKEHCIFTGDTLFVGDVGRPDLLDGLMTKEELASKMY
                     DSLNQKIKPLADDVIVYPAHGPGSACGKSMGKETFSTIGIQKQTNYALQEMTREAFIA
                     AVTDGLTQPPPYFFEDARINKNGYTSIDEVIANNTKALSIDAFKHAIENGAIILDTRV
                     ADNFEKGYVPGSLNIGLNGMFAVWVGTLVDIHLPLVLVTEQGKEEESIIRLARVGYEQ
                     VIGYLEGGIENWINKGEAIDSVTTIQAEQMSQFYNNPEYVVLDVRKPSEFDTEHVVNA
                     INIQLSEIDERAGELDNTKKYIVHCAAGYRSMMAASILKEKGFDNFVNVAGGWGSIKK
                     TSLPTEASVATLI"
     gene            complement(12140..13450)
                     /locus_tag="PLB46_02665"
     CDS             complement(12140..13450)
                     /locus_tag="PLB46_02665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HRF75446.1"
                     /translation="MSQKELIMPKLGESIMEATILKWLKKEGDSVELDEAVLEIATDK
                     VDSEVPSPFAGKITSILFKEGDVVQIGKAIAIIDGAGNGNGAAVIETPVKQEVKTEAP
                     KQVVVETKVEAAPVIAGDRFYSPLVLNIAREEGISMSELERIPGTGKDDRVTKSDILN
                     YLQNRGNVTTQQTVQVEKPVTQAPVVQEKSSPVVQTVSSNGNNEIVEMDRMRKLIADH
                     MVMSKKTSPHVTSYVEVDMTPIVEWRNRIKPKFEKREGQKITFTPIFIEAIAKAIKDF
                     PGINISVDGYNLIYKKDINIGMAAALPTGNLIVPVIKNADRMNLVGLTAAVNDYATRA
                     RNNKLKPDEIQDGTFTLTNVGSFGSLMGTPIINQPQVAILAVGAIKKKPVVLETVHGD
                     VIAIRHMMFMSMSYDHRVVDGMLGGSFLKRVADYLEGFDIEREI"
     gene            complement(13553..>13807)
                     /locus_tag="PLB46_02670"
     CDS             complement(13553..>13807)
                     /locus_tag="PLB46_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRF75447.1"
                     /translation="TLMGYAIATHKFFDDGFAITGNAGPTADGKAEDVGTVFIAVGSK
                     TEKRVKKHFFNKNREVNIEYASMFSLHELRILLTEYLVKA"
BASE COUNT         4292 a         2534 c         2510 g         4471 t
ORIGIN      
        1 atccgcaatc acaaaccctc aaatttgatc ctgaattaaa atacatttcc cattgggttc
       61 ctgaattaaa tgaattaagt taccctaaac ccattgttga ccatagtttt gcacgcgata
      121 gagtgctaaa ggtttacaaa gcggcattgt ccagataagg tgcaagtgta aaatatttta
      181 agaagatttc cttgtgttca ttttcaggat ttattactag gaacacccgg atattatcaa
      241 gcggtgcaat aggatattct tccagattga tttggacaac aaacatgtgg tgcatttttt
      301 ctactttata tgcataattc ggtaagtagt gattattgac actcacttcc acatataaag
      361 gcaatttttt tgcctcgcga aaatcaatat tgttttgaat aatacataaa tactttttat
      421 cgccgatatc aattgcctga tcaatataat cccaaccgca attagttatg cgtcgatgtt
      481 tattggttac tacttgaagt tcaaggttta ataactgatt agttacttcg gcagctgtaa
      541 aaaaggtatc caaattgttg taataagtaa tatttgaatc aattaactcc gacctgccat
      601 atccatctgc atacacattg attatttttc gtttaaataa catcaggcta ttgtaatttg
      661 aattcgagtt gttctttcct aaaccatcga tgtaatcttc aattaatgaa tcaatggtta
      721 tttccggctg aattaacttt tcaatccttg catcatcact tttcatcttg attaagctat
      781 ttaaccttaa ttctatcatt gatttagtga tgctattggc aatttgtaat tgatattcag
      841 ttgaactatc cggtttagat ttattgcaag cgcataaaaa taatgtggct aaggaaaaca
      901 gtaccaaggt gcatttcatt gtttaacaac gaatgcccta taattttaat tatcaataat
      961 gcctaaatct tgaaaatttg cgacagtgat ttctggtatg ttaatagtga attgggctaa
     1021 agttggtagg gaattgacgt tctgtcaata aaagtaaggt ttgctaaaaa ttaattgaaa
     1081 ttagggcaga tttttaatct taaattaata tatttgtaat cacgggcagc gatgcctcat
     1141 tgcacactcc aactttagta aattgtcgta aaccaatatg cccaactgga tatattattt
     1201 tatgccacat aatgtggtta ataaactcga aaaacgcaga cttacctatg cgttatgggt
     1261 gatatcaggt ttaggtatat tttttattct tgtattgatt gtccacacgg cttgcaaaat
     1321 gacgccggtg cattattatg ctgatgcatt tggtttaatt gggtcctatc ttgctgcatc
     1381 gtttataaaa aaaaataagt tggtattggc tgtcaaaaca attgtgatta cactattggt
     1441 tgcaatttta gtctacggac ctatagtcga ttatgttgaa cccgggcctg taatgttctt
     1501 aaggttgtat gtaaccatat ttgcacttga agccgcttta ctcctcatgg tttcttttta
     1561 tattgccata ctcaattatg tttatctggc tttattattt accagttatg tattactgca
     1621 cttttttgtt atcgtgaata aaaatggttg ggaaactgtt aatcctgaaa tgatttcata
     1681 cggtgtaatt ggtgttttag ccatcaatat ttcatgtttt atagcctcac tcatgttgcg
     1741 gtataataat gatttaatga ataaaattaa tgccgataat acagcaattg ttgaacataa
     1801 tatccgcctt gaagctgaag tgaaaaacag gaccagcgac ttacaaaaca gcaatgataa
     1861 cctcaaacaa tttgcgcaca ttgtttcaca tgaattaaaa gaacctttgc gcacaatttc
     1921 gggtttttca ggtttattac aaaagaagct ggataatttg aatgtagatg acccagatat
     1981 aaaagattat accaacacca ttcagaatag ttcaagacat atggcaaatc tggttaaaga
     2041 gatgctaaca ttttcttctg tacagcaatc acctaaatcg ctcgtggaga ttaaaatgaa
     2101 tgagctaatt gaattagttc tcaatcaaat tgataacatc gtccaggaaa aaaaagcgga
     2161 aattacctgg actgctttgc ccaatgcata tggtgaatca ttactggttt tccaacttgt
     2221 gcaaaattta ataattaatg gtattaaata ccaacctgaa aacaacactc cgaaaattac
     2281 tatatccgga agtcctaaag gccaattcat tgagtatgtt gttaccgaca atggtattgg
     2341 tatgagcaat gaaaccctgc ttaaaatttt cgacccgctt tatcgtgaac acaaacatac
     2401 ctatggcgac ggaaccggaa tgggcttatc aatttgtaaa aaaattgttg atgcacatca
     2461 tggcaaaatt tgggtgagta gtcagctaag cgcgggcacc tgctttcatt ttacgctgcc
     2521 tgcatacaat atacctatta cttaatgtaa gggtgcggat tcatttttat cttttttgtt
     2581 ggaactagtt tatggaattg taacaccttt tgcatcctat tgcaaaactt actacatgaa
     2641 ccgtctgctc attacattat tactccttac agggatgcaa ttacacgcac aaaaacaaat
     2701 tattgttgat gctcctgtag aaaaagccac tgtgttttta accggtgcac aattgttcca
     2761 ccaggctgca gtaaatctgc ctaaaggcca gtctgaaatt atattacaag gtttatccat
     2821 gtcgcttgac cctaattctg ttcaaggtgg agggaaaggg gaatttacta tcctggatat
     2881 tcaatatcgt ttgttttatc ccgaacctaa accgaatgca gttgtgctta cagaaacaga
     2941 taaaaaaatt aaatctatta atgattccat agttgaattg gattttgatt taagaacttt
     3001 ttataatagg agagaagtac tgaatgtcca aaaacaaatg atgctcaata atactttata
     3061 caaaggaact ggaagtgatt cgctcgtatt aattcagcaa agtgttgagt attatgataa
     3121 aaagttaaac gacatttatg tagaattatt acaagttgag aaaaaagaat atcaattaaa
     3181 tctaaaacga gctgctatgg ttgctcgagt atatgaatta caaaattacc gcaatcaaca
     3241 agctgctttg gagcaaccaa acaacccaat tcctcaaata ataatgagtg tgcaaactga
     3301 ttatgctttg agcgcaacag tggaagcaaa ttatattact tataatgccg gttggtatgc
     3361 tacttacgat attcgtgcaa ctgatatagc taaacctgtt gatattgcct acaaagcaaa
     3421 cgtgtggcaa aatagtggta tagattggaa agatgtaaaa ctcacctgtt caacaggaaa
     3481 tcctatgatt ggtaataatt taccggaaat tacaacctgg tatttagggt attatgatta
     3541 ctattataac cgtgatgaag ctaaaactat tacattaggt tctgttgcaa ccgaagatat
     3601 ggatgatgct aaagtattat ctgataaata cctaaacgca ccttcagctg ttgatgccgg
     3661 atatgctaat aattatacaa cacctgtgca aactattgcc aatgtggaat ttgatattca
     3721 attaaaatac tccataccta atgatggaaa aggacatatt gttgccttgc agacaaaaca
     3781 attaccaaca acatataatt acctgattgt accgaaagtg gaacaagccg catttttaat
     3841 cgcaagaatc accgattggg aaagtttaaa ccttttaccg ggcaatgcca acatctattt
     3901 caataatact tacgttggta aaaccaatat caatccattg gctttagctg atacactatc
     3961 attgtcttta ggtcgcgaca gggcaattga agtaaaacgc actcaattaa cagataagtc
     4021 gagcgaacga atattagcta ccaatgctaa aaagaccatg gcattcgaaa tagaaatccg
     4081 gaacggtaaa gcaattccaa ttgaggttat tattaaagac catatcccgg tatctcaaaa
     4141 agaaagcatt aaagtggaac tgtttgaaaa agatggtggt gagttggatg aattaacagg
     4201 aattattact tggagagaaa aactgaaaac taaagagaaa aaggatttca acctcgaatt
     4261 cgagatcacc tacccaaaaa acgagccact cagtttaaac taatcgcagc agttcgttgt
     4321 ctattaaaac ggccggtaat aatctaccgg ctgttttgtt ttgcaaagcc tcaagcatat
     4381 ggcttcaaat tggttataca ataggtcttg aggttactta tataacagaa cacggcatag
     4441 ggactttaat tgtcatcatt ttgtcttgta tcaactgctt ctattttccc cttaactgtt
     4501 gataattagg gcattatggg gtgagtgctg tggataaaaa tcagcctggt ttagaacttt
     4561 tccgactaat tttgtgtcat attattgatt taggtgagga aatacttgga atttcacgaa
     4621 tttacttgaa attaacctta aagtccgacc aaaatcagta attagcacta actttgcaca
     4681 ttaatttgaa aaaaaataaa atgaaaatac ttggggggtt aaatcgaatt gtttctttat
     4741 cattacttgg ttttctgcta agccttacta ctgcttcatt tgcaggggct ggagtaagta
     4801 aatcacctta tcataaagac cacaatccgc ttagttttta ttactttgga tacaatacta
     4861 tcaatcctgg ctttacctta ggaacgcata taaacttgtc atggactaaa atggagaaag
     4921 caggttgtcg tggttcaaaa atcagcgacc gtcagtttgt tatgattcca accatcggaa
     4981 tgtttcaaaa tgaagcaaac accctgagtg catatggcgc gttagagttt aactttaaca
     5041 ttactttcag caaaggttta accatgcagt ttttcggtgc tcccggttat gcccaaatgt
     5101 tatccggtga tgaaaccatc agtggtaacg ataatgccaa cagccgtgta ttaagtacaa
     5161 cagcacacag cggctttatg cctactgctg gtgcaggttt aggttataat tttcaaaaaa
     5221 tgaatggtaa agattgtcct ctggcaatca gcttccgagg tatggcaaca tcagttgatg
     5281 tagccaattt agcggtattt ccatcattcc aaacaggaat cacgtattct ttttaaagat
     5341 taattaagga taaatttttc tttggggata gaggttttgc tacctttatc cccaaattta
     5401 ttttaatgaa aaagtatgct tttttaatag tagctgtatt ggctactttt accgcttctg
     5461 cccaatatcc actagtgaat atagcagata tccaatttaa agacgatgct gcacttgctg
     5521 ctgaagatga tttatctgct tatgatggtg ataccgttcg tattcaggga attgtaacgt
     5581 ttgacccttg cgattatgcg ctttcaagtt caggaagccg catgggaacc taccttcagg
     5641 atcctgatga ggatgaattt gcaggaattc acgtattaat cgattggcct gctattggtt
     5701 ggacggacct cgagagttta aacgatgcta cgctgtttgt agataatttt caagtaggaa
     5761 atatcgtaga atgcactggt attgtgaata cctttgaggg taatacgcaa ttttggttaa
     5821 taccggttga atctgaaatc ataggatttg gcgtagttcc tgaccctgat gaacgtgaaa
     5881 ttgctgaatt tatggttagc gatggcgcag gcggacaggt tatcaatacc cttgatggcg
     5941 aaaaatatga gggcaaatat gttgaattta ctaacgttta tgtaacagac gttgttcctt
     6001 caggattgag atggttctgg tatttacaag atgatgccgg taataaaatc caaattcgcg
     6061 atatgagcgg acatttccgt aacgatactt atgatgatga atgtaaccta tgggctggtg
     6121 gagcagcagg tgaaactaat acgccattaa cctacgaacc gccggcaatt ggaaccaatt
     6181 tagcctatgt tcgtggtatt attctcgaat ttacggctga aacccaatat ggaattgctc
     6241 cattattatt atctgatatt ggaccttcat tggcctcacc tccggtaatt accaatatta
     6301 ccagaactcc aacagtggct actgcggctg aaacagttgt tgttagtgcc gatattaccg
     6361 atcttgacgg gttcgtgtca tctgccaatt tatattatag ttatggatat ggcagtacag
     6421 actggaacct ggcaactatg tcaaatgtag gtgctgattc ttgggaaggt gaaattccag
     6481 gaccagggat agcttttgat agcatgtatg ttaactttta cctggaagct tttgatgatg
     6541 atgccaatgt gattacaagt ccttcaccgg cttctccttt aacatatata gtatataatg
     6601 atggtattac ttctattgtg caaatccaaa atacaccttt cccttcagga agttcagttt
     6661 gggcaaatga tagtatacct acaatgaata tcgaagccat tgttacagca acaaccaaaa
     6721 cctacgactt gggtatggta gctgttcagg aaggaagtgg tcaatatgaa ggtattttta
     6781 ttaaatctgt tccgggtgat ggcactgacg ccttactccg tggcgataaa atcaaaatca
     6841 ccgctggaaa agtgattgaa gaatttggtg tgaccaaact taccaatatt acctatactt
     6901 tggtttcaca aatgaatgat ttaccggctt ttgttaccgg tataaaccca actgacgtag
     6961 atgctaaagt ttattcagct accgaacctt gggaagggat gttagtgaaa tttgataatg
     7021 cctttgttac atcaaacaac gcagatgctg ccggtggtgg tgcttttggc gaatggagag
     7081 tgaatacatc caatactcct gaagtaggaa tgcgttgcga cgattatagc tacgaaatta
     7141 actttgaatt tggtgttgat acattgacta tgggtgagga actcacttat attcaaggac
     7201 ttatgtatta tagcttcagc aactggaagt tattacctcg cgatagaaac gacatcgaag
     7261 gctatacaac agtgtatcca aacagcatag ttgctttcaa ctttaccagc ccggcagcta
     7321 ctggagcaat tgaccaggca gccggcacta tcaatttaac agtgcctatg ggaaccgacg
     7381 taaccgcttt aacaccgtct atcgatttta cgggacaata tgttgaccca gcctcaggaa
     7441 tagctcaaaa ttttactagt cctgtcgttt ataccagcta ttcaccagtt acttttgagc
     7501 caaaaaccta taccgttacg gtaacgcaaa atgttggcat cgagagtttc aacctgtcag
     7561 aaatacaggt ttatcctaac ccaacatctg acgttttgac tgttgaaatc ggggcaccta
     7621 cagacatgca ggtttctatt ggaattaaag acctggaagg gcgtaacatt caggaaatca
     7681 atgagttatg ctatgtggga aaaaatattt ttgcttttga tttgaggtca gttgcaaacg
     7741 gattatattt gcttcaaata agttcaaagg ataaaacaac gatacttaag atagaggttt
     7801 ccaaataaca ttttgccatt ggaaacctgt tttggttaac ccgtttcggt ttaaaatccg
     7861 taacgggttt ttttatgttt tgcattttca ttttaatagc agctaaaata aaattatcag
     7921 tatattttct gattaatcat ctttaccgga agctgttttt tattaatcga atatttttat
     7981 tgagtataaa atatcacctt gaaagtttgg tgcaaaaaaa atccggctca tttgaaccgg
     8041 attgttatga gttgtcgttt ttcaaaaacg ctcaagtttg ctaaaatgga aatcactttc
     8101 aatatttgca ttttcatcac tatcgcttcc atgaacagca ttttctccaa tgctggatgc
     8161 atattttttt ctgattgtgc cttcttctgc ttttgaaggg tctgttgcac caattaattt
     8221 tctgaagtct tctactgcat tatccttttc aagaatagct gctacaatag gacctgaact
     8281 cataaagtct acaagctcac cataaaaagg acgttcttta tgcacagcat aaaattcacc
     8341 tgctttctct ttacttaact tagtatattt catggcaatg attctgaagc ctgcttcatt
     8401 aatcatttgt aagattccac cgatggaatt ttttcgaact gcatcgggtt taatcatagt
     8461 taatgttcta tttcctgaca tattatagta ttttcggccg caaagatagg aattaactct
     8521 ctccattttt aaaaaatcta aaaaatggtg accttagcag taaatttaac atgaaagacc
     8581 tcggactcct cttcaatcat ttaaagcaac cacgcagaat tgtgttgctt actcatgtgc
     8641 gtccggatgg tgatgcagtt ggttcggcat tggcccttaa atcctatttt gaaataaaag
     8701 gccatacagc cgacgttatt gttcctgatg atttccctga ttttttgaat tggatgtttc
     8761 aaagtgaaaa ggcgtttaat catcaaaaaa acccaaaaac atgtttcgct gttttagcca
     8821 gggcagaagt aatttttttc ctcgacttta atgctttgaa aaggattgaa aatttagggg
     8881 aggatgtaaa aaaactgaac agaccatatg tgagtgtcat gattgatcat catcgtgaac
     8941 ccgattattt tactgactat atgttgtgga acgataaagc aagttcaacc gctgaattgg
     9001 tttacgattt tattcatatg gccggagatg ccgaattgat taataccgaa atggctgctt
     9061 gtatttatgc aggtatggca ttagataccg gtatttttca attcagtaat acaacctcgc
     9121 gtgttcatga aatagctgcg caattaatgc agaaaggaat aaatattgaa caaatacata
     9181 ataatgtata taatcaatat ggtgaaaaca gaatgcggtt tgtcgggttt ttattagctg
     9241 aaaaaatggt cgtagttgct gaacatcgcg cagcctatat gagtattacc atggcagaag
     9301 cagaaaagta taaattgaac aatggcgata aggagggtat tgttaaccta ccattagcca
     9361 tgcgcgatgt agacatggct gtgcttttta ctgaagatag agataaaatt aaaatatcat
     9421 tcaggtcaaa gggtgaagtg aatgttgaat ttttggcacg aaatttcttt aatggtggtg
     9481 gacataggaa tgcttcaggt ggttcttcca aattatcact ggatgaaact gtgaaacgat
     9541 ttgtagaagt gctacaggag tttgctcaac aacaaaacaa ttgatttaca taaaaacagt
     9601 tattcgaaat gaaaaaaaca agtttatttt tattgatgat tatgtttgtt caggcattag
     9661 cgtttggaca aagcgacaaa aagaaataca aacatttacc tgatgggttg tatgcagaaa
     9721 tgcaaaccag caaaggcaat attttgttat ttcttgaatt tcaaaaagca ccattaaccg
     9781 ttgctaattt cgttggtctt gcagaaggca aaattgaaaa tgcagctaaa ccattaggta
     9841 caccattttt tgatagcctg atttttcacc gcgttattgc tgattttatg attcagggtg
     9901 gtgatccgaa aaaaaatgga actggtggtc ctggttacgc attcaaagat gaattccata
     9961 gcgacctcaa acattccggt cccggaattt tatccatggc aaactcaggt aagtttacca
    10021 acggcagtca gttttttatt acgcataaag aaacaccatg gctggataat aagcatgctg
    10081 tatttggaca tgttgttgaa ggacaagacg tggttaatgc cattgctcaa ggcgatctga
    10141 tcgtgcatgt aaacattatc agcaaaggac aggcccttaa aaaaaacgat ccggcagctg
    10201 tcttcaaaga atttgaaaca aaaggagatg tgaaaagaag ttcaaaataa aaaaagggcg
    10261 aaagcccttt tttttatgca gtaaatttca atttttattt ataaaaccac ttcaaaataa
    10321 taaacctgat tggtttcagc attcctgtct tcaagcgtca tttcattatt ggtgagcgtt
    10381 aaaatattga acaccctcga ttgaaattca tcacttaatg ttaggatact tgctttatca
    10441 ctgaaaatat atataccggt aatactaaca ggtgttgccc ctaaattata tgtctcggta
    10501 taatctgaag tatcagtaaa tgtgatattg taattaagat aagtagaagt aaataatggt
    10561 gtttgatcat acccatcaat ttcaaattta tatacggacc atgatctgct aatatgatcg
    10621 gttgcctgat caacgcgaat gtcttcgcac gaagatgtca ggccgatcaa aaacacaatc
    10681 ataaatattt tccgcatatt aattcccatc atatcaaatt aatgttgcca ccgatgcttc
    10741 tgtcggaagt gaagtttttt taatacttcc ccaaccacct gcaacattca caaaattatc
    10801 aaatcctttt tcttttaaaa tagaggctgc catcatactg cgatatccag cggcacaatg
    10861 tacaatatat tttttagtat tatccaattc acccgcacgc tcatcaattt cgcttagttg
    10921 aatattgata gcatttacaa catgctcagt atcaaattca cttggtttgc gcacatctaa
    10981 cactacatat tcaggattat tgtaaaactg actcatctgc tctgcctgaa tcgtagtaac
    11041 ggaatcaatt gcttcacctt tgttaatcca attttcaatt ccaccttcta agtatccaat
    11101 cacctgttcg tatccaaccc ttgctaacct gatgatagat tcttcctctt tgccttgttc
    11161 ggttacaagt acaagtggta aatgaatatc cactaatgta cctacccaaa cagcaaacat
    11221 gccatttaat ccaatattta aagagcctgg cacatatcct ttttcaaagt tatctgctac
    11281 tcgagtgtct aaaataatgg caccgttttc aatcgcatgt ttaaatgcat ctatacttaa
    11341 cgctttcgta ttattggcaa ttacctcatc aatactggta tagccgtttt tattaattcg
    11401 agcgtcttca aaaaagtagg gtggtggctg agttaatcca tcagttacag cggcaataaa
    11461 tgcttcgcga gtcatttcct gcaaagcata attggtttgt ttctgtatac ctatagttga
    11521 aaatgtttct ttgcccatac tttttccgca tgcacttccc ggtccatgcg cagggtaaac
    11581 aataacatcg tcggctaaag gttttatttt ttgatttaat gaatcataca tttttgaagc
    11641 taattcttcc tttgtcatta atccatccaa taaatcagga cgaccaacat caccaacaaa
    11701 taacgtatca ccagtaaata tgcagtgctc ttttccctgc tcatcaatca ataaataaca
    11761 agtagattcc agtgtatgac cgggagtatg taatacgcga atttttattt taccaatatt
    11821 aaattcctga ccatctgtgg cattaattac agtataagca gtattggctt caggaccata
    11881 aataattgcg gcgcctgatt tttttgctaa atctaaatga cccgaaatga aatctgcatg
    11941 gaaatgagtt tcaaatacat atttaattgt tgcatttctg cttgcagctt tttctaaata
    12001 aggttccggt tcacgcagtg gatctattac agcagcaacg ccatcacttt ctatgtaata
    12061 agcagcttct gcaaggcaat tcgtgtatag ttgttctatg tgcatgttgt atattattgg
    12121 cgataaaaaa atcgttcaat taaatttctc tttcaatatc aaatccttct aaataatcag
    12181 ctacacgttt aaggaaactg ccgcctaaca tgccatctac aacacggtga tcataactca
    12241 tactcataaa catcatatgg cgaatggcaa taacatcacc atgaacagtt tccaatacaa
    12301 ccggtttttt cttaattgca ccaacagcta aaattgcaac ctgtggttga ttaataattg
    12361 gtgttcccat caaactgcca aatgaaccca cattggttaa tgtaaatgtg ccatcttgta
    12421 tttcgtccgg ttttaattta ttatttctag cacgcgttgc ataatcattt actgcagctg
    12481 ttaatcctac taaattcatg cggtctgcat ttttaattac aggaacaatt aaatttcctg
    12541 taggcaatgc agctgccatg ccaatattga tatctttttt ataaatcaaa ttatatccat
    12601 ccactgaaat attgataccc ggaaaatctt taattgcttt ggcaatggct tcaataaaaa
    12661 taggagtgaa tgttattttt tggccttcgc gtttttcgaa ttttggttta attctatttc
    12721 tccattcaac aatcggcgtc atatccactt caacataaga agttacatgg ggtgaagttt
    12781 ttttactcat caccatatga tcggcaatta atttgcgcat acgatccatc tccacaattt
    12841 cattgttgcc atttgatgac actgtttgta caaccggtga agatttttct tgcactaccg
    12901 gtgcttgtgt aaccggtttt tcaacctgaa cagtttgttg tgtagttaca ttacctctgt
    12961 tttgcagata atttaaaata tctgattttg taacacggtc atctttaccg gtgccgggaa
    13021 tacgttccag ctccgacata gaaataccct cttcacgggc aatattgagt actaaaggag
    13081 aataaaaacg atcaccagca ataaccggtg ccgcttcaac ttttgtttca actacaactt
    13141 gtttaggggc ttctgttttc acttcctgtt taacaggcgt ttcaattact gcggcgccat
    13201 ttccatttcc tgcaccatca ataatggcaa ttgccttacc aatttgaacc acatcacctt
    13261 ctttgaagag aatactggta attttacctg caaatgggga tggaacttcg ctatccacct
    13321 tgtcggttgc aatttctaat acggcttcat ctaattcaac agaatcacct tcttttttaa
    13381 gccattttaa aatggtggcc tccattatac tttcgcccaa tttgggcata atcagttctt
    13441 tttgcgacat gtttttctaa ttgctgttgt tagctatcta tttcgaaaca tgaaggtcgg
    13501 gattcaacaa gtccgctcct caggtttaat tttcgcaaaa atactgtctt tattatgctt
    13561 taacaaggta ttccgtcaac agtatgcgta attcatgtaa agaaaacatg cttgcatatt
    13621 cgatattcac ctcacggttc ttgttgaaaa aatgtttttt cacccttttt tccgtttttg
    13681 agcctaccgc aataaacact gttcctacat cttcagcctt gccatccgct gtcgggcctg
    13741 catttccggt tattgcaaat ccatcatcaa aaaatttatg tgtggcaatt gcgtacccca
    13801 tcagtgt
//
LOCUS       DAORKW010000033        13714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_63_length_13714_cov_5.815579, whole genome shotgun sequence.
ACCESSION   DAORKW010000033 DAORKW010000000
VERSION     DAORKW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13714
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63_length_13714_cov_5.815579"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLB46_02675"
     CDS             <1..319
                     /locus_tag="PLB46_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75448.1"
                     /translation="KLVCYFSMGCEYCKLTAGKITGMSEKLSIDSNIVYLFMGDEKNL
                     NQFWEDSDSKKFTYYILPVEDFFKASGSHLPAVYFVENGKVVKKAAYRTLNDKDVATF
                     FGK"
     gene            complement(350..931)
                     /locus_tag="PLB46_02680"
     CDS             complement(350..931)
                     /locus_tag="PLB46_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRF75449.1"
                     /translation="MSNFIVSIYTEATPNPDSLKFVMNKMLLTGRSVDFERGDEVAFA
                     PLAEAIFNEFSFVKGVFIMNNFVTVRKDGDIDWFEVKSSIADFIKNWVSEGKVIVGEI
                     PETDNISGEMDGDAVVAKIKAMLDQYVKPAVEMDGGAIQFKSFDNGIVTLMMQGSCSG
                     CPSSTVTLKSGIEGLLRRMVPEVKEVVAESVEM"
     gene            complement(1128..3710)
                     /locus_tag="PLB46_02685"
     CDS             complement(1128..3710)
                     /locus_tag="PLB46_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF75450.1"
                     /translation="MKNYFYLIILSLLTQSLVAQNQIAGNVSDINGGPLPGATIVQKG
                     STNGTVADLDGNFKLAINSMPATLVFSFVGYATQEIEVSAQTTLRVLMEPESYNLSGV
                     EVVGTRSLNRSVTESMVPVDLIDVASLTQANGQLDVNQLLQYTAPSFNSNRQSGSDGA
                     DHIDPATLRGLGPDQTLVLINGKRYHQSSLINIFGTRGRGNTGTDLNTIPAAAIERIE
                     ILRDGASAQYGSDAIAGVINIVLKDNVNEFTGNVNTGAYLAQHNFDVGAIDGENLQLN
                     GNYGFKIGERGFVNVTADYHYRGHTNRAEFTGDFPDHTDVRNQYGDAEVADFSAWFNM
                     NVPLNANTHFYAFGGSGNRNVEAYAYTRAADEDRNVIDIYRKGFDPIIASVVNDHSLS
                     TGLRGDIKGWDVDFNMTTGKNKMHYYGRNTLNASLGTASPTEFDDGGFSLMQSSTSID
                     FSRYFSEALKGLNIAYGAEYRIDNYTIFAGEEGSWYNYGGLFIDGGDTTTRPGGSQGF
                     PGFQPSDVTDEYRSNVGVYLDVEADITNDFALSAALRYEDYSDFGNTITGKLAARYAI
                     SDKYAIRGSVSNGFRAPSLAQIYFSSTYTNVENGQIIDELIADNKSVITRAAGIPGLT
                     EEKSINGSFGITAKPTTALSITLDAYYVTIADRIVLTDGFGTDDDSIGATLEELNVGY
                     ARFFTNAVDTKTMGLDAIISYKIYMNDNTLTLTYAGNFNQMEVTAIHTNELLAGKEDN
                     YFSDREEYFLLASAPPVKMHFNVEYATQKLNVNLRANYFGAINLINYSVEEYTYSPKT
                     TLDASIGYDITSNMHLTIGGTNLLNTYPDDTDPYETETGGAWDAVQMGFNGLFMYTKL
                     GFKF"
     gene            complement(3912..5354)
                     /locus_tag="PLB46_02690"
     CDS             complement(3912..5354)
                     /locus_tag="PLB46_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRF75451.1"
                     /translation="MKRLQFIFTFCLFAVLQSAVFGQNPDNSIRIKIDANVDGKTVKI
                     DTNISSLEDLDIDAYLRELGIDDELNQLNIDINSGFSFHWDEAAFDEMMEGLQSIEIP
                     EMPELHLEGLDDLAFISPNKAVLGVYTEKTPEGAEITGLVKDGAAAEAGLMEGDIITG
                     IDNRTIESPSNLSEVMALYEAGNSVKVTYLRNGKSETKNIVLKENKPAIDTWIEDWSN
                     EFDSIQFNWEGLNNGKIYEVMVPERGYLGVYLDDAENGVEVTGVEENSPAQKAGLQEG
                     DIITSLNDVDVEDYDALMDVMNDTKPGENVEIEFIRNGKKEKTSATLDEVKGGNFYFN
                     FPDEGNEPNIIIDHVAPLPPNCSYSYNSEDGNRNVTICITAVKDNEAVSTENNSTKLT
                     HPLMDPKNLVVYSNPSDGAFNIKFNLNEEGDTKIVITDINGKEVFTETLKNFSGVYDK
                     TISLSDAPKGTYFVKVTQNGYSGTKTVVLQ"
     gene            5525..7138
                     /locus_tag="PLB46_02695"
     CDS             5525..7138
                     /locus_tag="PLB46_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF75452.1"
                     /translation="MLKNRIWLIVIFMSTALLGIILVQVYWIRNTISQKEQVFNFHVN
                     EALNKVADKVETNIAASVVSSQMNLFFSDSTYWSTSAGSLEIYDGMMSDSMIAIALEA
                     ASAYGVDYFRANAKTAEPFGHAVSPMPSGLDRPTMILEPLEYNGDFRSADDALITDIL
                     TDIDRQLRINSQRIKKAMEQMMFQLMQRGIEPEQTIDTVFLKNTLYHELGNKGITTDF
                     NFGVLMDDKFFITNAGNNESIKELAATSHKVSLFPDDMFFNNDVLLVNFPHQKSFILS
                     SIWSLLIGSLLFTSIIIGVFYYTVLVLLRQKKLSEIKNDFINNMTHEFKTPLATISLA
                     VDAVNNPMILKDETKVKHYTHIIKEENKRMNAQVEKVLQMALLDKNEISLSTDDIDIH
                     EIIYRAIENISLQVEEKGGKIDMELAAERYEMTGDEVHLSNVISNLLDNANKYSPENP
                     QIKVTTESDDKGIYITIADNGIGMNSEDIKMIFEKFYRVPTGNVHNIKGFGLGLTYVK
                     AIIDAHHGTIEVKSQPKKGSQFKLFLPLT"
     gene            7152..7856
                     /locus_tag="PLB46_02700"
     CDS             7152..7856
                     /locus_tag="PLB46_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF75453.1"
                     /translation="MEEIKTKILLVEDDPNFGAVLKDYLSMSGYDVKLATDGEKGEDL
                     FFKEEFNLCVLDVMMPKKDGFTLAAQIKRINPEMPVIFLTAKTLQDDVLEGFKVGADD
                     YITKPFNSEELLYRIAAILKRGGKLRTNNQQKEFEIGKYHFNYKTRMLKNGSSDAKLS
                     PKEAELLRLFCLKVNDIVYREEALKQIWGRDDYFTARSMDVFVTKLRKYLKDDPSLEI
                     VNIHGNGFRLNTEEVV"
     gene            complement(8251..9948)
                     /locus_tag="PLB46_02705"
     CDS             complement(8251..9948)
                     /locus_tag="PLB46_02705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRF75454.1"
                     /translation="MKFSSYPYSRPDIEQLKADFKTLLNNFNASTTAAMQLDAMKAIN
                     NLRSQFDTMYQICSIRHSIDTRDDFYAAEQDFFDENTPSVEALASEYYQALITSPFRE
                     AIEAHFGKQLFRIAEMQLRAFKPEIIEDLIIENKLSTEYQELIANASIPFDGEEKTLA
                     EMTPYLRSTDRSVRERAANSRWQFFSDNEAALDRIFDELVKTRHRIAQKLGFQNFVPV
                     GYLRMMRSDYNPNMVADFREQVHQQIVPIAIALKQKQAKRLGLPALKFYDEPLSFKSG
                     NATPKGTPEWIIANGKKMYAELSPETADFFNFMLENELMDLVAKKGKAGGGYCTYISG
                     LKSPFIFSNFNGTSADIDVLTHEIGHAFQVYMSREFETSEYYWPTSDGAEIHSMSMEY
                     FAYPWMDNFFNGEADKYRFSHLVDSLLFLPYGVAVDEFQHGIYENPHWTPMERKQFWS
                     SIEKKYQPYKDFDGNKFLTQGGFWQTQAHIYQSPFYYIDYTLAQICAFQFWLKARQDK
                     NAAFTDYVKLCKAGGSKSFLELVDYAGLRSPFQKETVSEIVHEINHYLSQVDDLQLN"
     gene            10185..11216
                     /locus_tag="PLB46_02710"
     CDS             10185..11216
                     /locus_tag="PLB46_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75455.1"
                     /translation="MHLNSIDLNDPNSASRKKDHIELAFESQTSALQNDNRFYYEPLL
                     QGHSGDKTLAKSFLGKTLQTPIWISSMTGGTEQAGKINRHLAQACNEYGMGMGLGSCR
                     RLLDDNTYFEDFNLRPIIGDAQPFFANLGIAQIETLLEKGEADKIHALVAKLNADGLI
                     VHVNPFQEWLQPEGDHFRNSPLETIQKLLELVDYQIIVKEVGQGMGPESIKALLKLPI
                     AALDFGAYGGTNFSRLELLRSSQQHASLYRDLAYIGHTAVEMTGFVNAAVDTLGADLK
                     CKEVIISGGIGGFLDGYYGMEKVVLNSIYGQGSAFLKPAQQDYQALKTYIESQREGLA
                     LCSAYLKVK"
     gene            11286..12635
                     /locus_tag="PLB46_02715"
     CDS             11286..12635
                     /locus_tag="PLB46_02715"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HRF75456.1"
                     /translation="MAKIKGFSKLSKTEKIAWLAKHFLTSNLLDIAREFSSYMHSNAE
                     MQKVFDGISENTLTNFYLPYGVAPNFVINGRTYVVPMVIEESSVVAAASAGAKYWSDR
                     GGFHAEVISTTKVGHVHFLWRGNSEKLQAFFDTELKQILIADVKHLTENMEARGGGLK
                     SIELIDCTHLEPEYFQLKATFETMDAMGANFINSVLEEFASCLTEEVKANTDFTGAEK
                     TVEVVMSILSNYTPECVVKAWVECDVNALGTFDEGHLNAQQFARKFSTAIRIAKHDMY
                     RATTHNKGIFNGIDAVILATGNDFRAIEACGHAYASRNGKYESLSSCEVKDNKFIFSL
                     EIPLAVGTVGGLTKLHPLSKRSLEMLGNPSAKELMKIIACVGLAQNFSAIKALVTSGI
                     QKGHMKMHLTNILSHFGATESEIQLAATYFSDKTVSFSNVRAYLNDLRAGKLLTEKG"
     gene            12706..13332
                     /locus_tag="PLB46_02720"
     CDS             12706..13332
                     /locus_tag="PLB46_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75457.1"
                     /translation="MKIIYQRISALLFGAFMLFIISCNKSIYPPTSSVATTSQQENSL
                     SAITEFNPGNSPSGLSQSETFNAEIAADSTPTPSIEKNVVPENSNEQIPIIKSLKDAP
                     VYVVEDDEYPSQRDVNEALFVAILGLLLLPTGISLIFSALSLGMSKKLQRKIEADPKL
                     AIYSKKTKVAKILSIIGIALYVALVILIIIAIFSFFNYLENDFTLFGL"
     gene            13390..>13714
                     /locus_tag="PLB46_02725"
     CDS             13390..>13714
                     /locus_tag="PLB46_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75458.1"
                     /translation="MQEFYAHGKLLISGEYLVLKGAMALAVPTKFGQKMQIIDEPGSE
                     NIIHWKSYDVNNNCWADVILSKDDLLPLRGSVKNIDETTLATLQKILTTIRQNQPNFS
                     RSAGTT"
BASE COUNT         4387 a         2533 c         2468 g         4326 t
ORIGIN      
        1 aaaacttgtt tgttatttca gtatgggttg tgaatattgc aaacttacag ccggtaaaat
       61 tacaggcatg tccgaaaaac tgtcaatcga cagcaatatt gtctatttat ttatgggtga
      121 tgaaaaaaat ctaaaccagt tttgggagga tagtgactct aaaaaattca cctactatat
      181 cttacctgta gaagattttt ttaaagcaag tggtagtcat ttacctgcag tttattttgt
      241 tgaaaatggg aaagtcgtaa aaaaagctgc ttatcgtact ttaaatgaca aagatgtagc
      301 cactttcttt ggaaaataac tacatcttca aaattaatta ttggaattat tacatttcta
      361 cactttctgc aacaacttct ttcacctcag gtaccattct gcgcaataaa ccttctattc
      421 ctgatttcaa tgttacagtt gaagaagggc aaccactgca agaaccttgc atcattaatg
      481 ttacaattcc attatcgaaa gatttaaatt gaattgcacc accatccatt tcaacagcag
      541 gttttacata ttgatccagc atggctttaa tttttgccac aactgcatca ccatccattt
      601 cgccacttat attatctgtt tcaggaattt cgccaacaat aaccttccct tcgctaaccc
      661 agtttttgat aaaatcagca atcgacgatt ttacttcaaa ccagtcgata tcaccgtctt
      721 ttctaacggt aacgaagtta ttcatgataa aaacaccttt aacaaagctg aattcattaa
      781 aaattgcttc tgccaatggt gcgaaggcta cttcatcccc acgttcaaaa tctacactac
      841 ggcctgtgag caacatcttg ttcattacaa attttaatga atccggattg ggagttgcct
      901 ctgtgtatat gctaacaata aaattactca tgttggttat cttttttttg ccgaacaaaa
      961 ttaggcatta attggttcct gacgaaactt atatcctctg tttatgccct atttgttaaa
     1021 acagctaccc ccatatttta gttcaaaacc tcaaacaaca gcaatacttt cattaaaaaa
     1081 gccccattgg attgaaccaa tgaggctttt tataaaataa tcagttatta gaatttgaaa
     1141 cccagttttg tatacataaa caaaccatta aagcccattt gaactgcatc ccaggcgccg
     1201 ccggtttcag tttcataagg gtctgtatcg tcagggtaag tattaagcaa gttagtgccc
     1261 ccaatagtta aatgcatatt ggaagtaata tcgtatccaa tgcttgcatc aagggttgtt
     1321 tttggtgaat aggtgtactc ttcaacacta taatttatca ggttgattgc tccgaaataa
     1381 tttgcacgca gatttacatt taatttttga gtagcatatt caacattaaa atgcattttt
     1441 accggaggtg cacttgccaa taggaaatat tcttctctat cgctaaaata attatcttct
     1501 ttgccagcta ataattcatt agtatgaatg gctgtaactt ccatttgatt aaaattacca
     1561 gcgtaggtaa gcgttagtgt attatcgttc atataaattt tatatgaaat aatagcatcc
     1621 aagcccattg ttttagtgtc gactgcattg gtaaagaaac gcgcatatcc aacatttaat
     1681 tcttctaatg ttgcaccaat actatcgtca tcagttccaa aaccatcggt taatacaatt
     1741 cggtcagcga tagtaacata ataagcatca agtgtaattg acaatgctgt agttggtttt
     1801 gctgttattc cgaaactacc gtttatactt ttttcttcag ttaatcccgg aatacccgca
     1861 gctcgggtaa ttacactttt gttatcggca attaattcat caataatttg tccgttttct
     1921 acattggtat aagttgatga gaaataaatt tgggctaatg aaggcgctct aaatccatta
     1981 cttactgaac cacgaattgc atatttatca ctgatagcat aacgggcggc taatttacct
     2041 gttattgtgt tgccaaaatc tgaataatct tcataacgca atgctgcact caacgcaaaa
     2101 tcatttgtaa tatcagcttc tacatctaaa taaacaccaa cattacttct gtattcatcg
     2161 gttacatcac taggctgaaa gccaggaaat ccttgtgatc cacctggtcg tgttgtagta
     2221 tcgcccccat caataaacaa accgccgtaa ttataccatg aaccttcttc accggcaaaa
     2281 atggtgtaat tatcaattct gtattctgca ccgtatgcaa tatttaatcc ttttaatgcc
     2341 tcactaaaat atcgtgaaaa atcaatactt gttgacgatt gcattaatga aaaaccaccg
     2401 tcatcaaatt cggtaggaga tgcagttcct aaagatgcat ttaaagtatt gcgaccataa
     2461 taatgcattt tattttttcc ggttgtcata ttaaaatcta catcccagcc tttaatatct
     2521 cctctcaatc ctgttgataa cgaatggtca tttacaactg aggcaataat tgggtcaaat
     2581 ccttttctat aaatatcgat aacgtttctg tcttcatctg cagcgcgtgt ataagcatat
     2641 gcttcaacat tgcggtttcc ggagcctcca aatgcataaa aatgtgtgtt ggcatttaat
     2701 ggaacattca tattaaacca tgcagaaaag tctgcaactt ctgcatcacc atattggttg
     2761 cgcacatccg tgtgatccgg aaaatcgcct gtaaattctg cgcggttggt atgcccacga
     2821 tagtggtaat cggcagtaac gtttacaaag ccacgttcgc caattttaaa tccgtagttg
     2881 ccatttagtt gtaaattttc accatcaatt gcgcccacat caaaattatg ttgtgctaaa
     2941 taagcaccgg tatttacatt tccggtaaat tcatttacat tatcttttaa tacgatatta
     3001 attacacctg caatagcatc agaaccatat tgtgcagaag cgccatcacg taaaatttct
     3061 atacgttcaa ttgccgctgc cggaatggta tttaaatctg ttccggtatt tccacggcca
     3121 cgtgttccaa aaatatttat gagagaactt tgatgatatc tttttccatt tatcaatacc
     3181 aacgtttggt cagggcctaa tccacgcaaa gtagccgggt caatatggtc agcgccgtcg
     3241 gaaccacttt ggcggtttga gttaaacgat ggagcggtgt attgtaataa ttgattaaca
     3301 tctaattgac cattcgcttg cgttaacgaa gcaacatcaa ttaaatcaac tggcaccata
     3361 ctttcagtaa ccgaacgatt taaacttctt gtgcccacta cctcaacacc ggataagtta
     3421 tatgattccg gttccattaa tacacgtaag gtagtttgtg cgctcacttc aatttcctga
     3481 gttgcataac caacaaacga aaaaactaaa gtggcaggca tactattaat ggccaattta
     3541 aaatttccat caaggtcggc aactgtgccg tttgtagagc ctttttggac aatagtggca
     3601 cccggcaaag gccctccgtt aatgtcagaa acattgccgg caatttgatt ttgcgcaacc
     3661 aggctttgtg tgagtaggga aagaatgata aggtaaaagt aatttttcat aacatgtggt
     3721 ttattgtaag gctaatgtaa atattttata tgaaaagtat cagccttagt caacacctga
     3781 ttgttaacca atcaggcgtt ttattttatg ataatgagaa cctcaaataa ccaagcaata
     3841 caacacctac ctcataaaaa aagccgacta tttaaatagt cggcttaatc tatttaatta
     3901 acccttaaga actattgtaa tacgactgtt tttgtgccgg aataaccatt ttgcgtcacc
     3961 ttaacaaagt aggtgccttt tggtgcatcc gataatgaaa ttgttttatc gtaaacacca
     4021 ctgaaatttt ttaatgtttc tgtaaaaact tctttcccat taatgtctgt aattacaatt
     4081 ttggtatcac cttcttcatt caaattaaat ttgatattaa aagcaccatc acttggatta
     4141 gaatacacaa ccagattttt aggatccatt aatggatgag taagttttgt gctgttgttt
     4201 tccgttgaaa ccgcttcgtt gtcttttact gcagtaatac atatagtcac attgcgattg
     4261 ccatcttcag aattataact atacgaacag tttggtggca acggtgctac atggtcgata
     4321 atgatatttg gttcatttcc ttcatcagga aaattaaaat aaaaattacc tccctttact
     4381 tcatcaagtg tagcagatgt tttttctttt tttccattac ggataaattc aatttctaca
     4441 ttttcaccgg gtttagtatc attcattaca tccattaatg catcataatc ttccacatca
     4501 acatcattta atgaagtaat aatatctcct tcctgtaaac ccgccttttg agccggagaa
     4561 ttttcttcaa cacctgtaac ttccacgcca ttttctgcat catctaaata tacgccaagg
     4621 tatcctcttt cagggaccat tacctcgtaa attttgccgt tatttaaacc ttcccaatta
     4681 aattgaatag aatcaaattc gttactccaa tcctctatcc aggtatcaat tgccggttta
     4741 ttttctttta aaacaatatt ttttgtttcc gatttaccat tgcggaggta agtaactttt
     4801 accgaatttc cggcttcata aagtgccatt acttcgctca aatttgaagg cgattcgatt
     4861 gttctgttat caataccggt aataatatct ccttccatta atcccgcctc agctgcagct
     4921 ccatctttta ccaaaccggt aatttctgca ccctccggag ttttttctgt gtaaactccc
     4981 aaaacagctt tgtttggaga aataaaagcc aaatcatcca atccttctag gtgaagttca
     5041 ggcatttcgg gtatttcaat gctttgaagc ccttccatca tttcatcgaa tgcggcttca
     5101 tcccaatgaa aactgaaacc actgttgata tcgatattca gctggttgag ctcgtcatca
     5161 atacctaatt cacgcaagta ggcgtcaatg tccaaatcct cgagggagct gatatttgtg
     5221 tctatcttca cagttttacc atcaacatta gcatcaattt tgatacgtat ggaattatcc
     5281 gggttttggc caaaaacagc agattgcaga acggcaaaaa ggcaaaaagt gaaaatgaac
     5341 tgtaaccgtt tcatgttttg attcgtttaa attgtgttta gtaatgctgg aagccttgtt
     5401 atgactaagc catgacaaat ttaacctact aaacgaagcg aagtagttaa atgtcaatta
     5461 aaagatgtta atgttgcgtt aaacaacttt ttccccgatt ttaacacttg taaatttgta
     5521 aaagatgtta aagaaccgaa tttggttgat tgtaatattt atgagcacgg ctttgctggg
     5581 tatcattttg gttcaggtat actggattcg gaatactatt tcgcaaaagg agcaggtgtt
     5641 taatttccat gtaaatgaag cactgaataa ggttgctgat aaagtggaga ccaatattgc
     5701 tgcttctgta gtgagcagcc aaatgaacct gtttttttct gattctacct attggagcac
     5761 aagtgcaggc tcgttagaaa tttacgatgg catgatgagc gatagcatga ttgccatcgc
     5821 actggaagcg gcaagtgctt atggcgttga ttattttagg gcaaatgcta aaactgcgga
     5881 accttttggg catgcagttt caccaatgcc ttcagggctc gacaggccaa ctatgatatt
     5941 agagcctcta gaatacaacg gcgatttcag gtcggcagat gatgctttaa ttaccgatat
     6001 tttaacggat atcgaccgcc agttgcgcat taactcacaa cgcattaaaa aagctatgga
     6061 acaaatgatg ttccaactga tgcaacgggg tattgaacct gaacaaacaa tagacactgt
     6121 ttttttaaaa aacaccttat atcacgaatt aggtaataaa ggcattacga ctgattttaa
     6181 tttcggtgtt ttaatggatg ataaattttt tatcaccaat gcgggtaata atgaatcgat
     6241 aaaagaatta gctgcaacat cacacaaagt gagtttattt cctgatgata tgttttttaa
     6301 taacgacgta ttattggtta atttccctca ccaaaaaagt tttattttaa gtagtatctg
     6361 gtcgctgctg attggttctt tattatttac ttccattatt attggggtgt tttattatac
     6421 cgttttggta ttgttgagac aaaagaaatt atcggaaatt aaaaatgatt tcatcaacaa
     6481 catgacacat gaatttaaaa caccattagc tactatttct ttggcagttg atgcggttaa
     6541 taatccgatg atattaaaag atgaaacaaa agttaaacac tatacgcata tcataaaaga
     6601 agaaaataaa cgtatgaatg cacaggttga gaaagtattg cagatggcat tgttggacaa
     6661 aaatgaaatc agcctgagta ccgacgatat tgatatacat gaaattattt accgagctat
     6721 tgaaaacatc agccttcagg tagaagaaaa aggcggaaag atagatatgg agctggctgc
     6781 ggagcgttat gaaatgactg gcgatgaagt gcatttaagt aatgtaatca gtaatttact
     6841 tgataacgcc aataaatatt ctcctgaaaa tccgcaaata aaagttacta cagaaagtga
     6901 tgacaaaggt atttatatca ccattgcaga caatgggatt ggaatgaaca gtgaagatat
     6961 taaaatgatt ttcgaaaaat tttatcgtgt gccaacaggc aacgtgcaca atattaaagg
     7021 ctttggattg ggactgactt atgttaaagc aattatagat gcccatcatg gcactattga
     7081 ggttaaaagt caacccaaaa aaggcagtca attcaaatta tttttaccac taacctgatt
     7141 aaaactattt tatggaagaa attaaaacta aaattttatt agtagaagac gacccgaatt
     7201 ttggtgccgt tttaaaagac tatttatcta tgagcggcta tgatgtgaaa ttagctactg
     7261 atggtgaaaa aggtgaagat ttatttttta aagaagaatt taatctttgt gtattagatg
     7321 ttatgatgcc taaaaaagac ggcttcacgc ttgctgctca aattaaacgc ataaatcctg
     7381 aaatgccggt aattttcctc actgccaaaa cattgcagga cgatgtactg gaaggtttta
     7441 aagtgggtgc tgatgattat atcaccaaac catttaacag tgaagaatta ttgtatcgta
     7501 ttgcagcaat attaaaacgc ggtggaaaat tgcgcaccaa taaccaacaa aaagaatttg
     7561 aaattggaaa atatcatttc aattataaaa cacgcatgct caaaaatggc agcagtgatg
     7621 ctaaattatc gccaaaagaa gctgagttat tgcgtttatt ctgtttaaaa gtaaatgata
     7681 ttgtttatcg cgaagaagcg ctcaaacaaa tttggggtcg cgacgattat tttactgcaa
     7741 gaagtatgga cgttttcgta accaaattac gcaaatacct aaaagatgac ccttcattgg
     7801 aaattgtaaa tattcatgga aatgggttta ggttgaatac ggaggaggtg gtttgatgta
     7861 ccaatgtgga gatgtaccta tgtaccaatg aaacagccgg tttggagtat acctttaatt
     7921 cggaacttcg gttggggtta ataaatatta acccaattaa atttataaat caattatccg
     7981 gttaggagta tccgtttaat tcggaacttc ggttggggtt aataattatt aacctaatta
     8041 aacgtattac tgtttccgcc tcggcggaaa cagccagaaa aaatggtaga atttaattct
     8101 tagcaattat caccatcacc ccgcgtaaaa accatccaac caatcaacca aggatgcgcc
     8161 aagcatccga aaaaattgga tggtttaatc aaaatgcact gctgaatcaa aaaccccatt
     8221 tgctaatttg ctaattacct aattagctaa ttaattcaac tgcaaatcgt ccacctgact
     8281 taagtaatga tttatttcat ggacaatttc agagactgtt tctttttgaa acggtgagcg
     8341 caaaccggca taatcaacta attctaaaaa cgatttactg ccacctgctt tgcataattt
     8401 tacgtaatcg gtaaaggcag catttttatc ctgacgagct tttaaccaga attggaatgc
     8461 acatatttgt gcaagtgtat aatcgatata ataaaatggc gactggtaaa tatgtgcttg
     8521 agtttgccaa aatccgccct gcgttaaaaa tttattgccg tcaaaatctt tatatggctg
     8581 atattttttt tctatcgaac tccaaaattg ttttctttcc attggtgtcc aatgcggatt
     8641 ttcataaatg ccatgttgaa attcatcaac agcaacgcca tacggtaaaa ataataagga
     8701 atcaaccaga tgtgaaaagc gatatttatc tgcttcacca ttaaagaaat tatccatcca
     8761 cgggtatgca aaatattcca tgctcattga atgaatttca gccccatcgc tggttggcca
     8821 gtagtattca ctggtttcaa attcgcggct catatatact tgaaacgcat gtccaatctc
     8881 gtgtgtaagc acatcgatat cagcacttgt tccattaaaa tttgaaaata taaatggcga
     8941 ttttaatcca gagatatacg tgcaataacc accacctgct ttaccttttt tagcaaccaa
     9001 atccatcaac tcattttcta acatgaaatt aaagaagtcg gcagtttcag gtgataactc
     9061 tgcatacatt tttttgccat tggcaataat ccattcgggt gttcctttag gagttgcgtt
     9121 gccgctttta aatgaaagcg gttcgtcata aaattttaat gcaggcaatc ctaaacgttt
     9181 agcttgtttt tgttttaatg caattgcaat aggtacaatt tgctgatgca cttgttcgcg
     9241 aaaatctgcc accatgttag gattataatc gctgcgcatc atgcgtaaat atccaaccgg
     9301 gacaaaattt tgaaaaccta atttctgcgc aatacgatgt ctggttttta ccaactcatc
     9361 aaaaatgcgg tccagtgctg cctcgttatc gctaaaaaat tgccatcttg aattagctgc
     9421 tctttcacga acactcctgt cggttgatcg caaataaggt gtcatttcag ccaaagtttt
     9481 ttcttcccca tcaaacggaa tagatgcgtt ggcaattaat tcctgatatt cagtacttaa
     9541 tttattttca ataattaaat cttcaattat ttcaggttta aatgcgcgca attgcatttc
     9601 tgcaattctg aataattgtt tgccaaaatg cgcttcaatt gcttccctaa acggtgaagt
     9661 tataagcgcc tggtaatatt cacttgctaa tgcttcaaca gatggtgtat tttcatcaaa
     9721 aaaatcctgt tctgcagcat aaaaatcatc tctggtatca atggagtgac gaatagaaca
     9781 aatttgatac atcgtgtcga attgacttct caaattatta attgctttca tcgcatctaa
     9841 ctgcattgct gcagtagtgg atgcattaaa attgttcagc aacgttttaa aatcagcctt
     9901 aagttgttct atatcgggtc tgctatatgg atatgatgag aatttcattg aattttcctg
     9961 ttaaaaacgc aaaattaagg tattgcattg ttaaggcgta gtaaacagtt gttatgctac
    10021 tcaattttat tcctaaaaca caaatacata gcaatgtcta actattagca cacgttttgt
    10081 gtttacacat gcagtagcat aactgaacat ttacatctaa acgcggttat ggtaaagtta
    10141 tttgaacggc agctgcggtt caaatagtga ttaaaaccgt aactttgcac ctcaattcaa
    10201 ttgatttgaa cgaccctaat tcagcttcac gtaaaaagga ccatatcgaa ttggcttttg
    10261 agagccagac ttctgcgttg cagaacgata accgttttta ttacgaaccc ttattacaag
    10321 ggcattccgg cgataaaacg ctggccaaaa gttttttagg caaaacgctt caaaccccta
    10381 tttggattag cagcatgact ggcggcaccg agcaggccgg gaaaattaac cgtcacttag
    10441 ctcaggcatg caacgaatac ggcatgggaa tgggccttgg cagctgcaga aggcttttgg
    10501 acgacaatac ctactttgaa gattttaatt taagaccaat aattggcgat gctcaaccat
    10561 tttttgccaa tttaggtatt gcacaaatcg aaaccttact ggaaaagggc gaagccgata
    10621 aaatacatgc cttggtggcg aaactcaatg ccgacgggct tattgtgcat gtaaacccat
    10681 ttcaggaatg gctacaaccc gaaggcgacc atttccggaa ttcacctctt gaaactattc
    10741 aaaaattatt agaactagtt gattatcaaa taattgtgaa ggaagtgggg caaggaatgg
    10801 ggccggaaag tataaaagcc ttattaaaat tgccaattgc tgccttagac tttggcgctt
    10861 atggtggtac caacttttcc cgcttagaat tgttacgttc cagtcagcag catgcatcct
    10921 tataccgtga tttagcgtat ataggacata cagcagtgga aatgaccggc tttgtaaatg
    10981 cagcagttga caccctggga gcagatttga aatgtaagga agtgattatc agtggtggta
    11041 ttggcgggtt tttagatggg tattacggca tggaaaaagt ggtgttgaat agtatttatg
    11101 gacaaggttc agcattcctg aaacctgcac aacaggatta tcaggcgttg aaaacatata
    11161 ttgaaagtca gcgggaaggt ttagctttgt gttcagcgta tttgaaagta aaataactgc
    11221 actttggtgc aaaaaaataa attaacgatt attattggct ttattgccgg ttagttaatt
    11281 agcatatggc aaaaattaaa gggttttcga aattaagtaa aacagaaaaa atcgcctggt
    11341 tggcgaaaca ttttctgaca agtaatctat tggatattgc gcgtgaattt tcaagttaca
    11401 tgcactccaa tgctgaaatg caaaaagtat ttgacggtat ttctgaaaat actctcacta
    11461 atttttattt gccttacggt gttgcaccaa attttgtaat taatggccgt acgtatgtgg
    11521 tgccaatggt aattgaagaa agttcagtag tggctgcagc aagtgcaggt gcaaaatact
    11581 ggagcgatcg cggtggattt catgctgaag ttatttctac caccaaagtt ggtcatgtac
    11641 attttttgtg gagaggaaac agtgaaaaat tacaagcttt ttttgatact gaattaaaac
    11701 aaatcctgat tgccgatgta aagcatctta ccgaaaatat ggaagcgcgc ggcggtggat
    11761 taaaaagtat agaattaatt gattgcacgc atttagaacc tgaatatttt caattaaaag
    11821 cgacctttga aacaatggat gctatgggtg cgaattttat taattcagtt ttagaagaat
    11881 ttgcatcgtg tttaacagaa gaagtaaaag caaataccga ctttacggga gcagaaaaaa
    11941 ccgtagaagt ggtaatgagc attttgagta attatacgcc tgaatgtgta gtaaaagctt
    12001 gggtagaatg tgatgtaaat gcattaggca cttttgatga aggccattta aatgcacaac
    12061 aatttgcacg taagttcagt acggcaatca gaattgctaa acacgatatg tatcgtgcaa
    12121 ctacgcacaa taaaggaatt tttaatggta tagatgctgt tattttggca accggaaacg
    12181 atttccgcgc aattgaagct tgcggtcacg catatgcaag tcgcaatggc aaatacgaaa
    12241 gcttatcgag ctgtgaagta aaagataata aatttatttt ttcactggaa attcctctgg
    12301 cagttggaac tgttggcggt ttaacaaaat tacatccgct tagcaaacgt tcactcgaaa
    12361 tgttgggcaa cccaagtgca aaagaattaa tgaaaataat tgcctgcgtt ggacttgcac
    12421 aaaatttttc tgctattaaa gctttagtta catccggcat tcagaaaggg catatgaaaa
    12481 tgcacctgac aaatattctc agccattttg gtgcaacgga aagtgaaatt caactagctg
    12541 caacttactt ttccgacaaa acggtttctt tcagcaatgt gagagcctat ctcaacgacc
    12601 tcagagccgg gaagttactt acagaaaaag gataaaatgc ccgaatagtt tgttaacttc
    12661 gtaacgatag tataacgagg ttttcctgtt catccatttt accttatgaa aataatttac
    12721 caacgtattt cagcattatt atttggggct tttatgttgt ttattatttc ctgtaataaa
    12781 tcaatctatc cacctacatc ttcggtagca actacctcac aacaagaaaa tagtttatct
    12841 gctattaccg aattcaatcc tggtaattca ccttcgggtt taagtcaatc tgaaacattt
    12901 aatgcggaaa tagctgccga cagcacacct actccatcaa ttgaaaaaaa tgtagtaccg
    12961 gaaaattcta atgaacaaat tcctataatt aaatcgctta aagatgcccc ggtttatgta
    13021 gttgaagatg atgaataccc ttcacaaaga gatgtaaatg aagcattatt tgttgctatt
    13081 ttagggctcc tattgttacc aacaggaata tctttaatat tcagtgcatt gtctttggga
    13141 atgagcaaaa agctgcaaag aaaaattgaa gccgatccca aattagccat atattccaaa
    13201 aaaactaaag tcgctaaaat actttccatt attggcattg cattatatgt ggcattagta
    13261 attttaataa taattgcaat atttagtttc ttcaactatc ttgaaaatga ttttacatta
    13321 tttggtttat aagttttatg taaactgata tggtgtaatt aactttacaa aaggttatat
    13381 ctttgtgtta tgcaagaatt ttatgctcat ggcaaactcc tgataagtgg agaatattta
    13441 gtgctgaaag gtgcaatggc attggcagtc ccaaccaagt ttggccaaaa aatgcagatt
    13501 attgacgaac caggcagcga aaatatcatc cattggaaaa gttatgatgt aaataataat
    13561 tgctgggctg atgtaatttt atcaaaagat gatttacttc cgctcagagg aagtgtaaaa
    13621 aatattgatg aaacaacact tgctacatta caaaaaatat tgaccaccat acggcaaaac
    13681 cagccgaatt tctcgcgttc agcaggcaca acac
//
LOCUS       DAORKW010000034        13488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_68_length_13488_cov_4.699099, whole genome shotgun sequence.
ACCESSION   DAORKW010000034 DAORKW010000000
VERSION     DAORKW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68_length_13488_cov_4.699099"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..1366
                     /gene="secY"
                     /locus_tag="PLB46_02730"
     CDS             11..1366
                     /gene="secY"
                     /locus_tag="PLB46_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRF75459.1"
                     /translation="MKKFIQTLKNIWSIEDLRKRLTYTIGLVLLFRFGSYVMLPGIDP
                     AIYGQAANVNGQSGLLGLINLFAGGGFSRASIFALGIMPYISASIAMQLLAIAVPYFA
                     RMQKEGESGRRKMNQYTRYLTVIVTALQASGYFAYLKNMSEQADFIVAPEGLFWFICV
                     IILTAGTLFTMWMGERITDKGIGNGISMLIMVGIVAGLPAAIINEITNRGADGGGGYI
                     PFVLEIAFMVLVIMGCILLVQGTRRIPVQYAKRVVGSRQYGGVRNYIPLKINAAGVMP
                     IIFAQALMFIPGIFAQINEDWSSSPVLAMLNDFQSVGYNVIYGLLIIIFTYFYTAIII
                     NPTQMADDLKKNGGFIPGVKPGKKTAEFIDNVLSRITLPGSIFLALVAVAPVITRVIG
                     VDQSFASFFGGTSLLIMVGVILDTLQQIESHLLMRHYDGLMKSGRIKGRTSSAIGVPQ
                     G"
     gene            1363..2175
                     /gene="map"
                     /locus_tag="PLB46_02735"
     CDS             1363..2175
                     /gene="map"
                     /locus_tag="PLB46_02735"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRF75460.1"
                     /translation="MIYYKTTDEIKLIRESSLLVSETLALIAGILQPGITTLELDTKA
                     EAFIRDNGARPSFKGYRGFAHTLCISVNDEVVHGIPSKREIKNGDVVSVDCGVYKNSF
                     HGDSAYTFLVGEVTMDAVNLAKVTKESVYKGIEMATVGNRIGDISYAIQNYAESFHGY
                     GVVRELVGHGLGKSLHEEPEVPNFGARGKGPKIQEGLVIAIEPMINLGTKNVVQENDG
                     WTIRTKDRKISCHFEHTIAVTKGKADILSSFEPIEAAVKRNKNLYQEVETVS"
     gene            2175..2393
                     /gene="infA"
                     /locus_tag="PLB46_02740"
     CDS             2175..2393
                     /gene="infA"
                     /locus_tag="PLB46_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRF75461.1"
                     /translation="MSKQGLIKQDGTITEALSNAMFRVKLENEHEIVAHISGKMRMNY
                     IRILPGDRVTVEMSPYDLTRGRIIYRYK"
     gene            2401..2517
                     /gene="rpmJ"
                     /locus_tag="PLB46_02745"
     CDS             2401..2517
                     /gene="rpmJ"
                     /locus_tag="PLB46_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRF75462.1"
                     /translation="MKVKPSVKKRSVDCKIVRRKGRLYVINKKNPKFKQRQG"
     gene            2543..2923
                     /gene="rpsM"
                     /locus_tag="PLB46_02750"
     CDS             2543..2923
                     /gene="rpsM"
                     /locus_tag="PLB46_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRF75463.1"
                     /translation="MARIAGIDLPKNKRGVIGLTYIYGISNSTSKKILASANIDESKK
                     VKDWNDDEINAIRSFINNELKVEGALRSEQQLAIKRLMDIGCYRGVRHRKGLPVRGQR
                     TKTNSRTRKGRKKTVANKKKAPKG"
     gene            2964..3362
                     /gene="rpsK"
                     /locus_tag="PLB46_02755"
     CDS             2964..3362
                     /gene="rpsK"
                     /locus_tag="PLB46_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRF75464.1"
                     /translation="MAKTTTAKKTVKKRIVKVEAEGQAHILASFNNLIITFTNMTGQT
                     ISWASAGKAGFRGSKKNTPYAAQVATSDAAKVAYDAGLRRVEVFVKGPGSGRESAIRS
                     IVSSGIDVTVIRDITPLPHNGCRPPKKRRV"
     gene            3392..3997
                     /gene="rpsD"
                     /locus_tag="PLB46_02760"
     CDS             3392..3997
                     /gene="rpsD"
                     /locus_tag="PLB46_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRF75465.1"
                     /translation="MARYTGPTTKKSRSFGESIFGYDKSFDKRKYAPGQHGQSRKKKS
                     LSEYGVQLKEKQKAKYTYGVLERQFRKTFEAATRRHGVTGEALLQLLEARLDNTVYRL
                     GIAPTRRGARQLVNHKHIEVNGTVCSTPSASLRPGDVITIREKSKDIEWLKEAASTAS
                     RKFSWLEWNPTQMKGVFVSYPERDQIPETIKEQLIVELYSK"
     gene            4022..5020
                     /locus_tag="PLB46_02765"
     CDS             4022..5020
                     /locus_tag="PLB46_02765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRF75466.1"
                     /translation="MALLSFQRPDKITLQKATDFEGTFEFKPLEPGFGVTIGNALRRV
                     LLSSLEGFAISSVKISGVDHEFSTITGVMEDITEIVLNLKQVRLKKELGGDENAVEKI
                     FISLKDKEEFRAGDIEKHTNSFRVTNPDLLICRMEPFVKLDIELTIQKGRGYLPAEEN
                     KNIDKTIGVIPIDAIYTPIKNVKYTIENTRVEQKTDYEKLVVEIKTDGTIHPEDAIKE
                     AARILIQHLMLVTDENITFHTSGEKEVNVVDEHTLHIRKLLKTPLEDLDLSVRAYNCL
                     KAAKINSLSELVKYDTNELLKFRNFGKKSLVEIEAMVLDKGLQFGMDLAKYKLEED"
     gene            5058..5411
                     /gene="rplQ"
                     /locus_tag="PLB46_02770"
                     /pseudo
     CDS             5058..5411
                     /gene="rplQ"
                     /locus_tag="PLB46_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(5616..6497)
                     /locus_tag="PLB46_02775"
     CDS             complement(5616..6497)
                     /locus_tag="PLB46_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75467.1"
                     /translation="MRPDKLNITTYATLAAMFLHQENSSAEVIYTDIEPDILIHNPGQ
                     IFILDINDDGIADFNFLFSTTHFAYFTYFGGINFYQLNAILALPQNDNAIAAFTGSGG
                     AYVYPYVIDEGISIGPVTGNFSDKSIQTMAYQFYAIIQSYFYYPIYIAGDWVFGETKK
                     YVGLQLHANDSIYYGWLRLDVATNNRSFTVRDFAFETVAETAISTFIPVAINQISPTY
                     NCDIYANANTIYINLHSTNNKNAIATIYNLEGRKILSKEINNGTNSISIENVPAGMYI
                     VNVNCEDQIKSETILLQ"
     gene            complement(6564..7475)
                     /locus_tag="PLB46_02780"
     CDS             complement(6564..7475)
                     /locus_tag="PLB46_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75468.1"
                     /translation="MKITSTNKFKPGKLASYSAMAGAFIAINSNADGQVLYTDVNPDE
                     TYSENGEFYELDLNLDGDIDFSLKVFDFSTAGLFYSGTLTWAGVLQGVIAYSYNGAIG
                     GSSTYMFPYALNNGITIDSDMNFNTNASFGTQVLALYLGIQDFYGSGGTLPFFTSPLG
                     EWEGKSEKFLALRLEDGGADYYGWARLSVDDNSHEFTIHDFAIKQSQDTPIETGETVG
                     VHTVIQNNQLTAYSFGSTINLVVKDLQTNGASVKVFNMEGQIVYQNDLNLSGMQINLD
                     KAATGNYTVQVVTVEDGVYSKTVNIQN"
     gene            complement(7612..8475)
                     /locus_tag="PLB46_02785"
     CDS             complement(7612..8475)
                     /locus_tag="PLB46_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75469.1"
                     /translation="MKKLYKSNKAGKLTAYSAMAGAFVAVGTDANALVVYNDIDDVTY
                     TLGEGYGLDLDDNGYPDFLMQAISASSGNWTFASAIGALSSSGYGNPSNMIVGYTGAI
                     LPYGSALNAGEVIDSDSPFMSNTYNRVWLASIYSGGSYGQFGDAGDKYMGVMFDIDGE
                     LHYGWARLDITLSPVTVTVKDYAYEDVAEAGIEAGATTGGAVGIVALNENQVAAYSYG
                     STINVIVKDLNAGVNTVNVFDIDGRVVYTASVSGNNMSINLDNVATGLYTVQLTGAEN
                     AVFTKKLYIQN"
     gene            complement(8595..9479)
                     /locus_tag="PLB46_02790"
     CDS             complement(8595..9479)
                     /locus_tag="PLB46_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75470.1"
                     /translation="MKSNTHSNKKLLHYATSAAAFLAINNLQATVVYTDLDPDIMVGG
                     EGAEISLDINSDGNDDFGFFVYSFTGVGTYYGINFTYDFKLAAVSAQNGNELFGSLVT
                     YSSYSAVYSPVLPAGEGINSGDPFAEGGGTLGISLMVSLSGFPYYDYQAGNWAGVNMG
                     YMGFRINIDKDHYYGWMRVSVNEEATLITIHDYAYENEANKAIFTGQTATAIDENPLS
                     ETAIYSNGTNVFVALPEQIKAETTVNIFDLTGKMVKNFNGVTGSVTLDCDDLPSGNYV
                     VYITEGELSIKKQVNLTK"
     gene            complement(9571..12129)
                     /locus_tag="PLB46_02795"
     CDS             complement(9571..12129)
                     /locus_tag="PLB46_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRF75471.1"
                     /translation="MGKRIAKKVLLIGWDAADWKVISPLMDAGHMPTLEKLVNGGVMG
                     NFATLDPPLSPTLWTSIATGKRPYKHGIIGFTEPDPSGKGIRPAYITSRKVKAIWNIF
                     NQHNMKSHVIGWWPSHPAEPINGTMISNLYQRASKPLGEPWPMLDGTVHPAEKSEFYK
                     KLRIHPGELTAAHILPFIPDAKNIDQEKDKRLGMLTKIIADCSTIHAAATYILENEEW
                     DFLGVYYDAIDHFCHGFMKFHPPQMPMVPDDIYNLYKDVVISGYKYHDMMLERLLQLA
                     GPDVTVMLISDHGFHPDHLRPIELPKEPAAPALEHSPYGIFVLNGPGIKKDERIYGAS
                     ILDVTPTILTMYGLPIGADMDGNVLVNAFEEQIIPDKIDSWDEIPGNSGEHPKDKQED
                     PYAAAEAMEQLIELGYIERPSENIEKAIKSTVNENNYYLARSYMNGRKYNEAIEILTK
                     LFEENPSTTRYGMRLAHAYQSINKVAETRDVLSKIKNVQQGRESISMRIMEGNLLLIE
                     NRPKDAMELFEQIEKDMPNVARVNMQLGRCYLALKKWKKAEVAFVKELEYDPGNASAH
                     HGLGVTFLRRKNYDEAIDCFLNAVGLTYFFPFAHYHLGEALYFSGEYERSAEAFEVCL
                     KMAPGINKARQMLSTIYGEHLKKPEIAMDHKAKIQDYIKGDIVIVSGLPRSGTSMMMQ
                     MMDKGGLEIFTDGIRKPDENNPKGYYEHELVKSLARNKNWVKDAQGKCVKIISQLLFE
                     LPANYNYKIVFMERDLDEVIQSQHKMLVRDGKAKEDALNIRVLNVFKQNLERVHRWVP
                     AQSNMEIIFVSHRNLVNNPKEELQKVNDFLGKTLNIDEMVGVVDKSLHREKSNK"
     gene            complement(12201..12398)
                     /locus_tag="PLB46_02800"
     CDS             complement(12201..12398)
                     /locus_tag="PLB46_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75472.1"
                     /translation="MEKKQGNKITITTTDGQPFKVPVEGSLGLLALGYKGLIAWRQVR
                     RQYQKVREANKPQNENTKTED"
     gene            complement(12547..>13488)
                     /locus_tag="PLB46_02805"
     CDS             complement(12547..>13488)
                     /locus_tag="PLB46_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF75473.1"
                     /translation="ATGKTLPNYVLNPGGNFTADPAIVINGATGEIDLDASTPGGPYT
                     ISYTSPGCPETQTFTITINPLPAPSITAPEVVCLEGDPVILIGEPTGGVFSGTGVVGE
                     EFSPAFANATGSYITTYTYTDANGCTNSASTVIDVIQNSVDAGLDQYIEEYTTTVIYA
                     AGGSTFLWEPPAGLSCVDCATPIFDSLFSNTYTVTSWDDYGCIASDEVSIFVVPVFDT
                     EIFVPNTFTPNGDNMNDYLFAFGTDLESILSMQVYDRWGELMFSTENIDPNTQSDGWN
                     GYYKGIEASQGVYVYMIEVELKEGVRQQIQGNVTLIR"
BASE COUNT         4297 a         2451 c         2622 g         4118 t
ORIGIN      
        1 agcttaatac atgaaaaaat tcatacaaac attaaaaaat atttggagca tcgaagatct
       61 ccgtaaacga cttacctata ctatcggttt agtgttgttg tttcgttttg ggtcttatgt
      121 tatgcttccg ggtatagatc cagctattta cggtcaggcc gctaacgtta acggtcagag
      181 tggtttgttg ggtttaatta acttatttgc aggtggtggt ttcagcagag cttccatatt
      241 tgcattgggt atcatgcctt acatttcggc atctatcgcc atgcaattat tggctattgc
      301 agtgccctat tttgcacgta tgcaaaaaga aggtgaaagc ggtcgtcgta aaatgaacca
      361 gtatacacgt tacctcacgg taatagtaac tgctcttcag gcttccggtt atttcgctta
      421 tttgaaaaac atgtctgaac aggccgactt tattgttgct cctgaaggat tgttctggtt
      481 tatttgtgta ataattttaa ctgcgggcac cttatttacc atgtggatgg gtgaacgtat
      541 tacagataaa ggtataggta atggtatttc catgttaatc atggttggta tcgttgcagg
      601 tttaccggca gccattataa atgagataac aaatcgtggt gctgatggtg gtggtggata
      661 tattccattt gtacttgaaa tcgcatttat ggttttagtt attatgggct gtatcttatt
      721 agttcaggga acaagaagaa ttcctgttca atatgcaaaa agagtagttg gttcacgcca
      781 atatggtggg gttagaaatt atattccgtt aaaaattaat gcagctggtg tgatgccaat
      841 cattttcgca caggcattaa tgtttattcc gggtattttc gctcaaatca atgaagattg
      901 gagcagctca ccggttttag caatgttaaa cgattttcag agtgtaggtt ataatgttat
      961 atatggttta ttaattatca tattcactta tttctataca gcaattatca tcaacccaac
     1021 acaaatggca gatgatttaa agaaaaacgg tggttttatt cctggtgtta agcccggtaa
     1081 aaaaacagct gagttcattg ataatgtgtt gtcgagaata acattgccgg gatcaatttt
     1141 ccttgcactt gttgcagtag caccggtaat tacgagagta attggagttg atcagagttt
     1201 tgcaagcttt ttcggaggaa catcattact catcatggtg ggtgtaattc tggatacatt
     1261 acaacaaatt gaaagtcacc tgttaatgcg tcactatgac ggtttgatga agtcgggacg
     1321 aattaaaggt cgcacctcat cagcaatagg cgtaccacaa ggatgattta ttataaaaca
     1381 acagatgaga taaaactcat cagagagagt tctttacttg tttcagaaac gttagccctg
     1441 atagcgggaa ttttacagcc cggtatcact acacttgagc ttgatacaaa agctgaagcg
     1501 tttataaggg acaacggtgc tcgtccatca tttaaaggat atcgcgggtt tgctcataca
     1561 ttatgtatat cggttaacga tgaagtagtg catggtattc catccaaacg cgaaatcaaa
     1621 aacggggatg ttgtatctgt tgactgtggc gtatacaaaa acagttttca cggtgattct
     1681 gcctatactt ttttagtagg ggaagtaaca atggatgctg ttaatttggc taaagtgacc
     1741 aaagaatctg tatacaaagg cattgaaatg gcaactgtag gtaataggat tggagatatc
     1801 agttatgcca ttcaaaatta tgctgaatcg ttccacggtt acggagtagt tcgcgagttg
     1861 gttggtcatg gcttaggcaa aagtttgcat gaagaacctg aagtgccaaa ttttggtgcg
     1921 agaggaaaag gaccaaaaat tcaggaggga ttagtaatag ctattgaacc tatgattaat
     1981 ctcggaacaa aaaatgtggt gcaggaaaac gatggctgga caataagaac gaaggacaga
     2041 aaaatttcct gtcattttga acataccatt gcagtaacaa aaggaaaggc agatatttta
     2101 tcgagctttg aaccgattga agccgcggtg aaacgaaata aaaatttata ccaggaagta
     2161 gaaacagtta gttaatgtcg aaacaagggc taataaaaca agacggaacc atcacagaag
     2221 cattgtcgaa tgcaatgttt agagtgaagc tggaaaatga gcatgaaatt gtagctcata
     2281 tttcaggcaa gatgcggatg aattacattc gtattttacc gggcgacaga gtgacagtgg
     2341 aaatgagtcc gtatgattta acaagaggaa gaataattta ccgttataaa taacattgtt
     2401 atgaaagtaa aaccatcagt aaaaaaacgc agcgtcgatt gtaagatcgt gagaagaaag
     2461 ggtaggctgt atgtgataaa caaaaagaac cctaaattca aacaacgtca aggttaacat
     2521 ttaaaatttt tgaattaata atatggcaag gattgctggt attgatttac ctaaaaacaa
     2581 aagaggcgta ataggcctta cctacatcta cggaattagt aattctactt ccaagaaaat
     2641 tctggcttca gcaaacattg atgaaagcaa gaaggtaaaa gattggaacg atgatgaaat
     2701 taatgctatc cgctcattta tcaataatga gttgaaagta gaaggtgcat tacgttcaga
     2761 acaacagctg gctatcaaac gcctgatgga tattggttgc taccgtggtg ttcgtcaccg
     2821 taaaggttta cctgttcgtg gacagcgtac aaaaaccaat tcaagaaccc gtaaaggtcg
     2881 taagaaaaca gttgccaaca agaagaaggc acctaaagga taataattaa acagtaagtt
     2941 ttactacatc atttatttat aaaatggcta aaacaacaac agcaaaaaag acagttaaaa
     3001 agaggatagt aaaagttgag gctgaaggcc aggctcacat acttgcttct tttaacaacc
     3061 tgattatcac ttttaccaat atgactggtc aaaccatttc atgggcatcg gcaggtaaag
     3121 caggattcag gggatcaaaa aagaatactc cgtatgcagc tcaggttgca acatcagatg
     3181 cagctaaagt tgcttatgat gcaggattac gcagagtaga ggtatttgta aaaggaccag
     3241 gttctggtcg tgaatcagcg atacgttcaa ttgtatcatc aggaattgat gtaacagtta
     3301 ttcgtgacat taccccgctg cctcacaacg gatgtcgtcc tcctaaaaag cgtagagtat
     3361 aaactattaa acttttaaat ctaacagatc aatggcaaga tatactgggc ctacaacaaa
     3421 aaaatcaaga tcatttggcg aatccatttt cgggtacgac aaatcatttg ataagcgtaa
     3481 atacgcaccg ggccaacacg gtcaatcccg taaaaagaaa agcttgagcg aatatggtgt
     3541 tcagcttaaa gaaaagcaaa aagcaaaata cacttatggt gtattagaac gtcagttccg
     3601 caaaactttt gaagcagcaa ctcgtcgtca cggcgttaca ggtgaagcat tattgcaatt
     3661 actggaagct cgtttagaca acacagttta ccgtttaggt attgctccaa cacgtcgcgg
     3721 tgctcgtcag ttagttaatc acaaacacat tgaagtgaat ggtactgttt gcagtactcc
     3781 atcagcttct ttacgtccgg gtgatgtaat taccattcgc gaaaaatcga aagatattga
     3841 atggttaaaa gaagcggctt ccacagcatc gagaaaattt tcttggttag agtggaaccc
     3901 aactcaaatg aaaggcgttt ttgtaagcta ccctgaacgt gatcagatac ctgaaacgat
     3961 caaagaacag cttatcgtcg aactttattc taaataaaat acttaaaaaa caccaattaa
     4021 tatggcatta ctttcatttc agcgtcctga taaaatcact ttacaaaaag caacggattt
     4081 tgaaggtact ttcgagttca aacctttgga accaggcttt ggtgttacta tcggaaacgc
     4141 gttacgcaga gtattattaa gttcactgga aggttttgca atctcatcag taaaaataag
     4201 cggcgttgat cacgaatttt ctactatcac cggcgttatg gaagatatta ctgagattgt
     4261 cctcaacctg aaacaggttc gcctgaaaaa agaattaggt ggtgatgaaa atgcagtaga
     4321 aaaaatcttc atttctctta aagataaaga agaattcaga gccggtgata tcgagaaaca
     4381 taccaatagt ttccgtgtaa ctaatcctga tttacttatt tgcagaatgg aacctttcgt
     4441 taaattggat attgaattaa ctatccaaaa aggaagagga tatttacctg cagaagaaaa
     4501 taaaaacatt gataaaacaa tcggagttat tccaattgat gctatttata cgccaattaa
     4561 aaacgtaaaa tacactattg aaaatacccg tgttgaacaa aaaactgact acgaaaaatt
     4621 agtagttgaa attaaaactg atggaactat tcatccggaa gatgccatta aagaagctgc
     4681 aagaatttta attcagcact taatgttggt taccgatgaa aacattactt tccataccag
     4741 tggcgaaaaa gaagttaatg ttgttgatga gcatacttta catatccgta aattgttaaa
     4801 aacaccactt gaagatttag atctttcggt tcgtgcttat aactgtttga aagcagctaa
     4861 aatcaactca ttgtctgaat tagtaaaata tgatactaat gaattgttga aattccgcaa
     4921 cttcggtaaa aagtcgttgg tagaaattga agcaatggta ttagacaaag gtttacaatt
     4981 cggaatggac cttgctaaat ataaattaga ggaagattaa tcaagatgtt gtgaaacatc
     5041 gataaaaaaa ttgaataatg agacacggaa ataaaataaa ccacctcgga agaaagtacg
     5101 cacacagaaa agccatgtta ggtaatatgg gtgtgtcgct catcatgcat aaacgcataa
     5161 aaacaacttt ggcaaaagct aaagaactta gagtgtatat tgagccgatt atcaatcgcg
     5221 caaaagaaga caatacacat aaccgtcgtg aggtgttttc acaccttcag gataaagaag
     5281 ctgttaacga gctttttact gaaattgcag gcaaaattgc aggacgtaat gggggttata
     5341 cccgcatttt aaaaattgct gaaactcgtg ttggcgatag cgctgaaact gcttatattg
     5401 agttagttga ctacaacgaa aacatgttga agacagctaa aaccgcaact aaaaaacgca
     5461 gtcgccgcgg atctggtaaa actgataaaa aagcagttga agcagaaaca aaaacagaaa
     5521 ccaaagatga agaaactaca gaagacaaag ctgcagaata aagcttttat taatattact
     5581 caaacggggg tgaggatttt cttcatcccc gttttttatt gcagtaaaat ggtttcagat
     5641 tttatttgat cctcgcaatt tacatttacg atatacattc cggccggtac attttctata
     5701 gagatactat tagtgccatt attaatttct tttgacaata ttttacggcc ttctaaattg
     5761 taaatggtgg caattgcatt tttattattt gtgctgtgta aattgatata tatagtgttt
     5821 gcgttagcat atatatcaca attatatgtc ggtgatattt gatttattgc caccggaata
     5881 aaagtcgata tcgcagtttc ggcaaccgtt tcaaaagcaa aatctctcac tgtaaacgat
     5941 cggttatttg ttgcgacatc taaacgtaac caaccataat agatgctgtc gtttgcatgc
     6001 agttggaggc cgacatattt ttttgtttct ccaaacaccc aatcgccggc aatataaatt
     6061 gggtaataaa agtaagattg aataatggcg taaaattgat aagccattgt ttgtatagat
     6121 ttatcagaaa aattaccggt taccggtcca atcgaaatgc cttcgtcaat tacataggga
     6181 tatacataag cgccaccact tccagtaaaa gcagcaatag cgttatcatt ttgtggtaat
     6241 gctaaaattg cattcagttg ataaaaatta atccctccaa aataggtaaa ataggcaaaa
     6301 tgtgttgttg agaataaaaa attgaaatcg gcaataccat catcattaat atcgagaata
     6361 aatatttgcc cgggattgtg gattaagata tcgggttcaa tgtccgtata aattacttct
     6421 gcagaactat tttcctgatg taaaaacatt gcagctaaag tagcatatgt agtaatattt
     6481 aatttgtcag gacgcattag aaattaaata tacaaaaaaa cctccgaatt tttcggaggt
     6541 tttaactttt gaataggcta aaattagttt tggatattaa ctgttttgga ataaacacca
     6601 tcttcaacag taacaacctg cacagtataa ttaccagttg cggctttgtc taaattaatt
     6661 tgcataccgc tgaggttcaa atcattttgg taaacgattt gtccttccat gttaaatact
     6721 tttacagatg cgccgttagt ttgtaaatct ttaactacca ggttgatagt agatccgaaa
     6781 ctataagcag ttaactggtt gttttgaatt acagtatgta ctccaactgt ttcaccagtt
     6841 tcaattggtg tatcttgtga ttgtttgatt gcaaaatcgt ggatggtgaa ttcatgagag
     6901 ttatcatcaa cagaaagtcg ggcccaaccg taataatcgg ctcctccatc ctctaatctg
     6961 agtgctaaaa acttttctga ttttccttcc cattccccaa gtggagaagt aaagaatggt
     7021 aatgttccgc cagagccata aaaatcttga attccgagat atagcgccaa aacctgcgta
     7081 ccaaatgacg cattggtgtt gaaattcata tcagaatcaa tagtaattcc gttatttaaa
     7141 gcatatggaa acatgtaggt agaagaacca ccaatagcac cgttgtaaga ataagcaatt
     7201 acaccttgta atacgccagc ccaagtgagt gtaccactat aaaatagtcc tgcagtactg
     7261 aaatcaaaca ctttaagcga aaagtcgatg tcgccatcaa ggtttagatc taattcataa
     7321 aactccccat tttctgaata ggtttcatca gggttaacgt ctgtgtacaa cacctgacca
     7381 tcagcatttg aatttattgc aataaaagct ccagccatag ctgaatagga agcgagttta
     7441 cccggtttga atttgttagt agatgtaatt ttcattttta cagattaaag gttaattaat
     7501 tatagagcga atgtagtaat ttttttacat ttaaaaataa ccaaatgtca tttttatata
     7561 aataaaaagc ccccgaaaat cgggggctaa aatgatatta cacctaaaaa attaattctg
     7621 aatgtataat tttttagtga atacagcgtt ttcagcgcca gttaattgaa cagtatataa
     7681 gcctgtagca acattgtcaa ggttaattga catgttattg cctgatacag atgcagtata
     7741 tactacacga ccatcgatat cgaatacatt aacggtgttc acaccagcat tcaaatcttt
     7801 aacaataaca ttgattgtgc taccgtagct ataagcagca acttggtttt cgtttaaagc
     7861 aacgatacca acagcgccac cagtagttgc accagcttca ataccagctt cagcaacatc
     7921 ttcgtaagca taatctttaa cagtaacagt tacagggcta agtgtaatgt caagacgagc
     7981 ccaaccatag tgaagttcac catcgatatc gaacataaca cccatgtatt tgtcgcctgc
     8041 atcaccaaac tgtccgtaag aaccgcctga ataaatagaa gctaaccaaa cacggttgta
     8101 agtgttagac ataaacggag aatctgaatc gattacttcg ccagcgttta atgcactacc
     8161 gtaaggtaaa atagcgccag tgtaaccaac gatcatgttt gatgggttac cataacctga
     8221 agaacttaaa gcaccaatag ctgaagcgaa agtccagtta ccagaactag ctgaaatagc
     8281 ttgcattaag aaatcaggat aaccattgtc atcaagatct aatccgtaac cttcacctaa
     8341 ggtataagta acgtcatcaa tgtcgttata aacaacaaga gcgtttgcgt ctgtgccaac
     8401 tgcaacgaat gcaccggcca tagctgaata agctgttaac tttccagctt tgtttgattt
     8461 gtagagtttt ttcatatctg cagattgttt taaaaaatcc agtgcgaatg taactctttt
     8521 ttttgagtac aacacggttt cagacaaaat ttatgacgaa aaaagccggg attttggtcc
     8581 cggctttggt gagtttattt ggtcaaattg acttgttttt tgatacttaa ttcaccctca
     8641 gtgatatata caacatagtt gcctgatggg agatcgtcgc aatcaagggt aacagaaccg
     8701 gtaacaccgt tgaaattttt aaccatttta ccggttaaat cgaagatatt cacggttgtt
     8761 tcggctttta tttgctcggg taacgcaaca aatacatttg tgccattgct gtaaattgct
     8821 gtttcagaaa gcgggttttc gtcaatagct gttgcagttt ggcctgtgaa aatggcttta
     8881 ttggcttcat tttcataagc ataatcgtga atagttataa gtgttgcctc ttcatttaca
     8941 gaaacgcgca tccaaccgta atagtggtct ttatcgatat tgatacggaa acccatataa
     9001 cccatgttta ctccggccca atttcctgct tgataatcat aatatgggaa gccagataag
     9061 gataccatta agcttatacc aagtgttcca ccaccttctg caaatgggtc accactatta
     9121 atgccttcac ctgctggtaa aacagggctg taaacagcgc tataacttga ataagtgact
     9181 aaactaccaa atagctcgtt accattttga gcagaaacgg cagccaattt gaaatcgtaa
     9241 gtgaaattaa taccgtagta agttcctaca ccggtaaatg aataaacgaa gaaaccgaaa
     9301 tcatcatttc catcactatt gatgtcgaga cttatttcag caccttctcc accaaccata
     9361 atatccggat caaggtcggt atatactaca gttgcctgta aattattaat ggctaaaaat
     9421 gctgcagcac ttgtagcata gtgcaataat tttttgtttg agtgcgtatt gcttttcata
     9481 cttttaattt ttattaacga agataccggt tgttgtttat ttgatttatt aaaacttgtt
     9541 aatgttcaac atctcagtgt atgctatgtt ttatttgttt gatttttcgc ggtgaaggct
     9601 tttgtctacc actccaacca tttcgtcaat gtttaaagtt ttaccgagaa aatcgttaac
     9661 tttttgaagt tcttcctttg gattatttac caagttgcga tgggaaacaa aaataatttc
     9721 catgtttgac tgagcaggta cccaacgatg aacgcgttca agattttgtt taaatacatt
     9781 taatactcgg atatttaaag catcttcttt agccttacca tcacgcacca acattttatg
     9841 ttgactttga attacttcat ctaagtcgcg ctccatgaaa acgattttgt aattataatt
     9901 ggcaggtaat tcaaataata actgactaat tattttcaca cattttccct gtgcatcttt
     9961 cacccagttt ttattacgtg caagtgattt tactaattcg tgttcgtaat aacctttagg
    10021 gttattttca tcaggtttgc gaataccgtc ggtaaatatt tcaaggccac ctttatccat
    10081 catttgcatc atcatactgg tgcctgatct aggtaaacct gaaactatta cgatatcacc
    10141 tttaatgtaa tcctgaattt ttgctttgtg atccatcgca atctcaggtt ttttgaggtg
    10201 ctcaccatat attgtgctca acatttgacg agctttgtta atacccggcg ccatttttaa
    10261 acatacctca aatgcttccg cactgcgttc gtattcacct gaaaaataaa gtgcttctcc
    10321 caagtggtaa tgtgcaaacg gaaagaagta agtaaggccg actgcattga ggaaacaatc
    10381 aatagcttca tcgtaatttt tgcgacgtaa aaatgtaaca cctaaaccat ggtgtgctga
    10441 tgcattaccc gggtcgtatt ctaattcttt aacaaatgct acttcagctt ttttccattt
    10501 ttttaacgcc aaataacaac gaccaagctg catattaacg cgtgcaacat tcggcatatc
    10561 tttttcaatt tgctcgaata attccattgc atcttttggc ctattttcaa ttaataataa
    10621 attgccttcc ataatgcgca tactaattga ttcacgacct tgttgtacgt ttttaatttt
    10681 agataaaacg tcgcgggttt cggcaacttt attaatggac tggtatgcat gagctaatcg
    10741 cattccgtaa cgcgtagttg aagggttttc ttcaaacaat ttcgtcagaa tttcgatggc
    10801 ttcattatat tttcttccgt tcatgtagga acgggcgagg taataattat tttcgtttac
    10861 agtactctta attgctttct caatattttc acttgggcgt tcaatatatc cgagttcaat
    10921 taattgttcc attgcttcag ctgccgcata tggatcttcc tgtttgtctt ttgggtgttc
    10981 accggaattg cctggtattt catcccaact atcaatttta tcaggaataa tttgttcttc
    11041 aaatgcattt acgagcacgt taccatccat atcggcacca attggaagtc cgtacatggt
    11101 taaaatagtt ggtgtaacat caagaattga agcgccataa atacgttcat cttttttgat
    11161 gccaggtccg tttaaaacaa aaataccata agggctatgc tctaaagcag gtgcagccgg
    11221 ttcttttggt aattctatcg gacgtaaatg atccgggtgg aaaccgtgat cggaaataag
    11281 cataactgtt acatccggtc cggcaagttg taacaaacgt tccaacatca tatcgtgata
    11341 tttgtaaccg ctaattacta catctttata taaattataa atatcatccg gaaccattgg
    11401 catttgtggt ggatgaaatt tcataaatcc gtgacagaag tggtcaatcg catcataata
    11461 aacgccgagg aaatcccatt cttcattttc aagaatatac gtagctgctg catggattgt
    11521 tgagcagtcc gcaataattt tagtaagcat tcccaagcgt ttatcttttt cctggtcgat
    11581 attttttgca tcaggaataa aaggtaaaat atgggcagca gttaattcac ccggatgaat
    11641 acgtaatttt ttatagaatt cagatttttc agcagggtgt acagtaccgt ctaacattgg
    11701 ccaaggctca cctaaaggtt ttgaagcgcg ttggtataag ttagaaatca tggtaccatt
    11761 aatcggctca gcaggatgtg aaggccacca tccaataacg tggcttttca tattatgctg
    11821 attaaaaata ttccagattg cttttacttt tctggacgtg atatatgcgg ggcgaatacc
    11881 tttacctgac gggtcaggtt cagtaaatcc gatgattccg tgtttataag gacgtttacc
    11941 agttgcgatt gatgtccata aagttggaga aagtggtgga tctaacgtag cgaaatttcc
    12001 cataacgcca ccattaacta acttttccaa agtaggcata tgacctgcat ccataagcgg
    12061 gctaattacc ttccagtcgg cagcatccca cccgataagg agtacttttt ttgctattct
    12121 tttacccatg atgtttttgt gtctttatta atacagatga ttgttgaggt gtgtttgggg
    12181 cctttatgca atcatactga ttaatcttca gttttagtgt tttcgttttg tggtttgttc
    12241 gcttcgcgaa ctttttggta ttgacgacga acctgacgcc atgcaattaa ccctttatag
    12301 cccagcgcta ataaacccaa agaaccttct accggaactt taaaaggctg accatcagtt
    12361 gtggtgatgg tgattttatt tccttgtttc ttttccattg atttgaatta gtaaagagtg
    12421 caaatttaac agatgccttt cgggattact aatttggact gatgatttta ctcaaagatt
    12481 aacttttggt taaaccatac caatattgat taatggtagg tattatgcac acccttatat
    12541 tgcagattaa cgaatgagcg taacgttgcc ttgaatttgt tggcgaacgc cttcttttaa
    12601 ttcgacttca atcatgtaaa catatacacc ctgactggct tcaatgccct tgtagtagcc
    12661 attccagcca tctgactgtg tatttgggtc aatgttttca gtgctaaaca tcaactcacc
    12721 ccaacgatcg taaacttgca ttgaaaggat ggattcgagg tcggttccaa atgcaaaaag
    12781 atagtcgttc atattatccc cgtttggtgt aaatgtatta gggacaaaaa tttctgtatc
    12841 gaatacaggg actacaaaaa tggatacctc atcagaagca atacaaccgt agtcatccca
    12901 agacgtaacc gtataggtat ttgaaaataa tgagtcgaaa ataggcgtgg cgcaatcgac
    12961 acaacttaac cctgccggtg gttcccataa aaaggttgaa ccgcctgcag cataaattac
    13021 tgtggttgta tattcctcaa tatattggtc aagaccggca tcaacactat tttgtattac
    13081 atcaattaca gttgaagctg aattcgtaca accgtttgcg tctgtgtaag tatacgttgt
    13141 aatataactg ccggttgcat tcgcaaaagc agggctgaat tcctcgccaa ctacaccggt
    13201 tccgctaaaa acaccacctg ttggttcgcc tattaaaata actgggtcgc cttccagaca
    13261 aacaacttca ggtgcggtta tacttggcgc aggtaaagga ttaatggtaa tggtaaatgt
    13321 ttgtgtttca gggcacccgg ggcttgtata agaaattgtg tatggtccgc ctggtgtact
    13381 ggcatccagg tcgatttcac ctgttgctcc atttattaca atggcagggt ctgcagtaaa
    13441 attgccaccc ggattaagta cataatttgg taatgttttg cctgttgc
//
LOCUS       DAORKW010000035        13391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_70_length_13391_cov_5.862702, whole genome shotgun sequence.
ACCESSION   DAORKW010000035 DAORKW010000000
VERSION     DAORKW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13391
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70_length_13391_cov_5.862702"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(447..1622)
                     /locus_tag="PLB46_02810"
     CDS             complement(447..1622)
                     /locus_tag="PLB46_02810"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75474.1"
                     /translation="MRIFLIVIIAVVVLLSAPQQVKAQAATFEVEFIGFTAESETAFN
                     YATDIWSNFLISDVPIKIVAHLQPLLPGQLGITFPNGEINFVDAPFLNYWYASCLANS
                     IAGTDLNGDDADIEIYISSTANWYYGLDENPGPGEYDFVSTVLHEICHGLGFLSLSNK
                     SGTEGSFGLIFASSFFPLTTTFIWPELDTMPGIFDKYLEDFSGVALTTYENPSTALGD
                     AQISNNLFFDSPEIILENDGIRPKIYAPATFTLGSSLSHWDETTYPVGSYNEFMTPKA
                     AANVADHMPGILTLTVLEEIGWEINYDTFQVDVVNIEPEIIIYPNPSHGILYIDNAQL
                     INATNYAIIDIQGKICKAGELVNNEINMLELKNGVYVIVLKSLDGEVVWRGVNVTTD"
     gene            1858..4359
                     /locus_tag="PLB46_02815"
     CDS             1858..4359
                     /locus_tag="PLB46_02815"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRF75475.1"
                     /translation="MFSGFNNINQSVWTIVLFLLFPVATFAQTRTIEGHVYDNETKQP
                     IPLATVIIKNTDFGVYTEEDGSFYINTALNNDTLLISYIGYYSDTIFLGKQQTQRFTV
                     FLKPQNFNLYEVVIKPREDPARVLMKKVIEHKKENDYYNIDGYKTRAYTKIELDLGNL
                     VLDNMNAEDNMRKKNPYNVLIDHIDTLNDETNYLPFFFTETLSDLYFRESPKSRKEVI
                     VASRTSGINNASASQILGNYYEQYNIYNNYWFIINKNFVPPVTDAWNFFYKVELTDSA
                     FIDNYWCYKINFSPRQKQENVFEGYMWIADDDFAVKEVYMSLDSAANINYYKRAVFYQ
                     EYEKVDDKHWVLKQDKILGEFLPMQNSASIIVRKSTTYQSYIFAADIFDEVVKLRDDI
                     VYSDSVINNDEQFWASIRPEALNKNETGVYELVDSLQNIKSFMTVVDLYNTLMYGYWN
                     LGYVRIGPFANMISSDEVEGLRLRLGAKTGDLISKRMSLGGYLAYGIKDNAFKYGGDF
                     NIVVNKKPWQQFKFSYENDLDVSSNEGVTFGEDNILSGFYRRRDTPQKILDHEKIRGH
                     YEKDWIWGISNSVTFTSVKMHPLFDIYYYNQHDSLVSDINNAELDFGLRFAYREKFLF
                     DNYRRYSLGTSYPVINFHYKVGIPDVGSSDFKYTAFEFSIDDYFLLGSFGATDYSIKA
                     GKVYGNLPTLLLESPPGNETYFMNHGNFNLMNEYEFVMDTYAELFLTHSFHGFFLNKI
                     PLIQKLKLREVATFKMVYGKLSADNAAANNYLKNVEPFYIGNAAPSPKPYMEAGIGVE
                     NIFKLIRVDAIWRLSYRNNPLAPNFGVRVGVSLDI"
     gene            complement(4424..7231)
                     /locus_tag="PLB46_02820"
     CDS             complement(4424..7231)
                     /locus_tag="PLB46_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF75476.1"
                     /translation="MFRRYTQLAGLLLIAQLASAQAVTTIPVFPTVDVPVTIIFDATQ
                     GNGALADVPGPIYAHTGLITTESDSPTDWQYTQGVWGTPDPEVLMTEIGDNLYQIEYT
                     SILDFYGATEDDTILQMAFVFRTASGDVVGRDTDGSDIFVNVYEDGLNVAITSPTTDP
                     FIIDLGDNIHVSAAAVFADSMYLYVNGAEAVATDLSSIEYDITAASFGLGEIRVVAKG
                     DGDIKVDSTYYYVIGDPIMADVPAGLHQGINYIDDNTVTLVLYAPFKSYIFAVGEHSN
                     WMLQESSFMNLDSDGATWWVTLTGLEAGKEYPYQYFIDGNLWVADPLAEKVLDPWNDS
                     YIPEVTYPGLIDYPTGKASGIVSVFQTAQPAYTWAIPEFTPPAVEDLVVYELLVRDFL
                     AARNYQTLLDTLSYLKALGINAIEFMPVMEFEGNESWGYNPSFFIALDKYYGTREAFK
                     IFIDSCHANGIAVILDIAMNHAFGQSPLAQMWWDPLANAPAANSPYFNTVPKHEYNVG
                     YDFNHESDATRNMRNIIFSYWLQEFNVDGYRFDLSKGYTQTNTLGDVGLWGQYDATRI
                     AIWKDIADVLYAVEEDAILILEHFAVNTEEEELSDYGFLLWGNSNYNYAQGTMGYTGS
                     DIHWMSYVARGWSDPHVMGYMESHDEERLMYKNLTFGNSGNPDYNVKYLPVALSRMEL
                     AGAFFFTIPGPKMIWQFGELGYDISIDYGCRVCNKPVLWNYYDIASRLRINQVWSELI
                     KLKTTYPAFSSTSFILNVAGSGKRINLNNDEMNVTVIGNFDVYNASVNPNFQHTGFWY
                     EYFSGDSINVSNISATINLLPGEYRLYTDVRLNTPEIIISIEELASAMQHELLVSPNP
                     SGNLFNLSAELPTSGEVAISIYNLKGELVFEDDIISNAGAFNYTWSGNTTDGSAIPTG
                     TYLCKMQTAAGVYLAKLMKVQ"
     gene            complement(7406..7825)
                     /locus_tag="PLB46_02825"
     CDS             complement(7406..7825)
                     /locus_tag="PLB46_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF75477.1"
                     /translation="MFPQRLSLLTIGVRNLDLMKNFYLNQFGWTLMLEQGDIVFFKLN
                     GFILSLYPSTELAKDIGIEAEAGNFKQITTALLLPSIEAVNALFEELIAKNVTVIKSP
                     EKVFWGGYSGYVADPENNYWEIAFNPFIEMDEAGNIQ"
     gene            complement(7827..8672)
                     /locus_tag="PLB46_02830"
     CDS             complement(7827..8672)
                     /locus_tag="PLB46_02830"
                     /inference="COORDINATES: protein motif:HMM:NF039362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HRF75478.1"
                     /translation="MGFLDRLSNGWKLSMSSFAVIKKHKQLLLFPVLSGASLLLLFAS
                     YLAILLLPNEGNFGAVVRERDIITYIGLFVFYLINYFIVVFFNMGLIHCARIYFQGGV
                     PKVSDGLNFSLSRLGDILAWSAMAATVGLIFRLLEENLGKVGQIIAALAGVAWSITTF
                     FVVPVLAYENLTPIAAFKRSAQIMKEKWGESLGSSFSFGIVQILGILIIGLPLFFLGS
                     LIGLIPSIVLAGIGILFVIAVTSAGQTIFISAVYHRINDNDVEDFDGDLLDSAFIHKE
                     KKGLF"
     gene            complement(8729..9649)
                     /locus_tag="PLB46_02835"
     CDS             complement(8729..9649)
                     /locus_tag="PLB46_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75479.1"
                     /translation="MKKILLILFVSINFFVHAQVDSLFQVPGTHCSMVPPKGFDKAET
                     FSGFQQPTTGSSIMITELPTNYMVIVNSFTADALAKAGLILINSDTIIFNEITAFTYL
                     IKQKANEVIFNKQVFIFGNDKQAVMVNGMYPENETAVGDIILSSMKSVRYNETTAENG
                     KEAVKFALDPTGTSMIYSGYVSGALMYSQDGKMPTQGSDNAVFTVGSSFASVTIGDTK
                     AYTQSRLKSLPYAEETVIKTINPITINNISGYEIVAEGKDREKKPQLIYFVMLYPDNE
                     SFYIMVGMTSAKYEDYLSDFRKIAQSFTLK"
     gene            complement(9653..9964)
                     /locus_tag="PLB46_02840"
     CDS             complement(9653..9964)
                     /locus_tag="PLB46_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HRF75480.1"
                     /translation="MENKWYTTEIGRFRTVAIFEGISFLLLLFIAMPLKYAANIPQAV
                     DIMGWIHGGLFILYMFTGLNVKMVHDWPFKKTLLAVVASIIPFGPFILDKKILQKEIA
                     K"
     gene            10079..10903
                     /locus_tag="PLB46_02845"
     CDS             10079..10903
                     /locus_tag="PLB46_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HRF75481.1"
                     /translation="MLLIGKKQRLKAIRFVPEGVFLDDGAEGVLLPRKQVPAGLRTDD
                     EVEVFIYNDGDGALMATTMEPKGVLDDIVALEVINVTPNGAYLQLGIPKDVFLHKSQI
                     RNIAEPGDIKVVKLILDYDGRLAATELIESWLDNSELTVIEKEQVSIIIIRETDLGYN
                     VAINNKHLGLLHYSDVFRHVKPGDVFTGHIKRILPENKLDVAEGKHGYQRVEDELGKI
                     LRLLDENDGFLPFHDKSEAEDIYAYFAMSKKTFKKAVGALYKQRIIRLSEDGIHKV"
     gene            complement(10923..11474)
                     /locus_tag="PLB46_02850"
     CDS             complement(10923..11474)
                     /locus_tag="PLB46_02850"
                     /inference="COORDINATES: protein motif:HMM:NF018866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HRF75482.1"
                     /translation="MITDEDVILHTKEWIKQVVIGCHFCPFAAAPVRNNAVRYVVMAN
                     AETAKLLQLILDECTYLDEHELTSTSFIIVPESLTDFRTYLDFLGMAEDIMKNSGFEG
                     VYQVAGFHPNYLFAGSDENDAANYTNRSVYPMFHLIREAEIDIALENYSEPMSIPERN
                     INFARKKGLAYMKMLRDSVMHTK"
     gene            11637..12110
                     /locus_tag="PLB46_02855"
     CDS             11637..12110
                     /locus_tag="PLB46_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75483.1"
                     /translation="MKPATLSDIKKELQLLDPLHVTELCIRMARFKKENKELLTYLLF
                     ESSNEQAFIEGIKEETIAFFETINDSNLYFAKKSIRKILRNINKHIRYSGNAATQVEL
                     LLFFCKSLKESGIPYHTSAAMVNLFDGQIKKINKALSTMHEDLQYDFKKDLKQLI"
     gene            12139..>13391
                     /locus_tag="PLB46_02860"
     CDS             12139..>13391
                     /locus_tag="PLB46_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF75484.1"
                     /translation="MSAEVILITPPFTQLNTPYPATAYLKGFLNTKNITAFQADLGIE
                     VILKVFTKNGLNKIFDTIQQQHPENADALSCLVLRNDYVATIEPVILFLQNNEPTLAH
                     RIAAGGFLPEGPRFQQYDNLDWAFGHMGVTEKAKYMATMYLEDIADIISTFVDPHFGF
                     SRYAERLARSANSFDELYQQLLNPPTFFDTILFEIIHQHILQHHPKILAISAPFPGNV
                     YAGFRCAQFVKQHFPQIKTVLGGGFANTELRAVADARVFEFFDFIILDDGEAPIEQLV
                     NFVRNNITIEELKRTFTLVNNKVVYINNSKCIDYKQFETGTPDYGDLLLDKYIAAIEV
                     INPMHSLWSNGRWNKLTMAHGCYWGKCTFCDISLNYIQAYEPLTAAVICDRMEQIMAQ
                     TGESGFHFVDEAAPPSLMRAVALEI"
BASE COUNT         4369 a         2617 c         2231 g         4174 t
ORIGIN      
        1 gttaccaata tgccaccctg acgggctcgt tgatgtgggg tctcaccaag tgatggtgtt
       61 attaatatgc cgccctaccg ggcttattga tgtgtggccg ccgacgctgt ggcgttaccg
      121 atatatcgcc ctctcgggct ggtttacttt aaagttggga tctattaata caatgtaact
      181 tcaaacagcc cgacagggcg tcattatggt ataacaaacc acacttcaat tagtcttcca
      241 ggccggcatc ttggtaacac cacactgcta tccgtcttcc agcccgatag ggcgacatat
      301 tggtaacacc actctgctat ccgtcatcca gcccgacagg gcggcatcta ggtaaccaca
      361 ccgtcatacc ccaaccagcc cgccagggcg acacactggt ttaccagctc cacattggta
      421 catcggtaca ttccctcatc ggtacattag tcggtggtca cattaacccc tctccaaaca
      481 acttcaccat ccaaactctt caacacaatt acataaacac cgttttttaa ctcaagcata
      541 tttatttcat tgtttactaa ttcacccgcc ttacaaattt ttccctgtat atcaataatt
      601 gcataattag ttgcattaat taattgtgca ttatctatat acaatatccc atgagaagga
      661 tttggataaa taattatttc aggttcaata tttacaacat caacttgaaa agtatcataa
      721 ttaatttccc aaccaatttc ctccaatacc gttaatgtta aaatacccgg catatgatcc
      781 gcaacatttg ctgccgcttt gggtgtcata aattcattat aactgccaac cggataagta
      841 gtttcatccc aatgtgataa acttgaacca agtgtaaacg ttgccggagc atagattttg
      901 ggacgtatgc catcgttttc taaaataatt tcgggactat caaaaaataa attgttactt
      961 atttgagcgt cacctagagc cgttgaagga ttctcataag tggttaatgc aacaccggaa
     1021 aagtcttcca gatatttatc aaaaatgccc ggcatggtat ccagttccgg ccaaataaat
     1081 gtagtagtta aaggaaaaaa cgaagaagcg aaaattaatc cgaaagaacc ttccgttccc
     1141 gatttattcg aaagacttaa aaatcctaag ccgtgacaaa tttcatgtaa tacggtggaa
     1201 acaaaatcat attctcccgg accgggattt tcatctaatc cgtaatacca attcgcagtt
     1261 gagctgatat aaatttcaat atcggcatca tcaccattta aatctgttcc tgctattgaa
     1321 ttagccaaac acgatgcata ccaataattg agaaaaggcg catcaacaaa attaatttcc
     1381 ccgtttggaa aagtaatacc caactgtccg ggaagcagcg gctgcaaatg cgcaacaatt
     1441 ttaataggca catcacttat caaaaaatta ctccaaatat ctgttgcata attaaatgcc
     1501 gtttcggatt ctgcagtaaa tccaataaat tccacctcaa atgtagctgc ctgagcctta
     1561 acctgttgtg gagccgagag caataccaca acagcaatta taacaattaa aaatatgcgc
     1621 ataatttcaa tttgtattca aaaatacaca atagttaatt ggttattaat catttataat
     1681 gtaattacgt cataaagctc attaaaattt ttcacaacat tttcgggttt agcaggagtt
     1741 attcttgtca caaaatgcct tacgctgtta ttagaaatcc ttattttcca agccttataa
     1801 aaaatctatg acaattttgt tttattttta ttgcagattc ggcgcagccc ttaaaatatg
     1861 ttttcaggtt ttaataatat aaatcaatcg gtttggacaa ttgtgttgtt cctgcttttt
     1921 cctgtagcaa cttttgcgca aacaagaaca attgaaggtc atgtttacga taatgaaaca
     1981 aaacaaccca ttcctcttgc tactgtaata attaaaaata ccgattttgg cgtttatact
     2041 gaagaagatg gtagttttta tataaacact gcactcaaca acgacaccct gttaatttct
     2101 tacatcggtt attatagcga caccatattt ttaggtaaac aacagacaca aaggtttact
     2161 gtttttttaa aaccccagaa ttttaattta tatgaagtgg taattaaacc acgtgaagat
     2221 cctgcgcgag ttttaatgaa aaaagtaatt gaacacaaaa aagaaaacga ttattataat
     2281 attgatggtt ataaaacccg cgcttatacc aaaatagaat tggatctcgg caacctggtg
     2341 ctcgacaaca tgaatgcaga ggataatatg cgcaaaaaaa atccttataa tgtattaatc
     2401 gaccatattg atacattaaa tgacgaaacc aactacttgc cattcttttt tactgaaacc
     2461 ttatctgatt tgtatttcag agaaagtccg aaatcgagaa aagaagtaat agtagccagc
     2521 agaacttcgg gcataaataa cgcaagcgca tctcaaattt taggcaacta ttatgagcag
     2581 tataatattt ataacaatta ctggttcatt ataaataaaa attttgttcc cccggttacc
     2641 gatgcctgga atttttttta taaagttgaa ctaaccgatt cggcattcat cgataattat
     2701 tggtgttata aaattaattt ctctccacgc cagaaacagg aaaatgtgtt tgaaggatat
     2761 atgtggattg cagatgacga ttttgctgtg aaagaagtct atatgagcct cgatagcgcc
     2821 gcaaatatta attattataa acgcgccgtt ttttatcagg aatatgaaaa agtggacgat
     2881 aaacattggg tgcttaaaca agataaaatt ttaggcgagt ttttacccat gcaaaattcg
     2941 gccagtatta ttgttcgtaa atccaccact tatcaatcat atatattcgc tgccgatatt
     3001 tttgatgagg tagtaaaatt gcgcgatgat attgtgtata gcgacagcgt gattaataat
     3061 gacgaacaat tttgggcatc aatcagaccc gaagcactca ataaaaatga aacaggggtt
     3121 tatgaattag tcgacagcct tcagaatatt aaaagtttta tgacagtcgt cgacttgtat
     3181 aacacgctaa tgtacggcta ctggaacttg gggtatgtgc gtatcggtcc attcgccaat
     3241 atgattagca gcgatgaagt agaaggattg cgattgcgct taggtgctaa aactggcgac
     3301 ctgattagca aaagaatgag ccttggtggt tatctggctt atggaataaa agacaatgcc
     3361 tttaaatatg gtggtgattt taatattgtt gtaaacaaaa aaccatggca acaatttaaa
     3421 ttctcgtacg aaaacgacct ggatgtcagc agcaacgaag gggtgacttt tggtgaagat
     3481 aatatcctat ccggatttta tcgccgacgt gatacaccac aaaaaattct tgaccatgaa
     3541 aaaattcgtg ggcattatga aaaagactgg atttggggta tttccaattc cgtgactttt
     3601 acgtccgtaa aaatgcatcc gctttttgac atctattatt ataaccagca cgattcattg
     3661 gttagcgata tcaataatgc cgaattagat ttcggtttgc gatttgctta cagagaaaaa
     3721 ttcttatttg ataattatcg tcgttatagt ttaggcacca gttatccggt tattaatttt
     3781 cattataaag ttggtattcc cgatgttggt tcaagcgatt ttaaatatac cgcattcgaa
     3841 tttagtatcg atgattattt tttactgggt agttttggcg ctaccgatta ttccattaaa
     3901 gccggaaagg tgtatggtaa tttacctaca ctgttgttgg aaagtcctcc gggaaatgag
     3961 acttatttta tgaaccacgg caatttcaat ctcatgaatg agtatgaatt tgtaatggat
     4021 acttatgcag aattattttt aacacatagc ttccacggtt tttttctcaa taaaatcccg
     4081 ttaattcaaa aacttaaatt gcgcgaagta gcaacattta aaatggttta tggcaaatta
     4141 agcgccgaca atgctgctgc taataattat ttaaaaaatg tagaaccctt ttacattgga
     4201 aatgctgcac cttcaccaaa accttatatg gaagccggta ttggagtaga aaacattttc
     4261 aaacttattc gtgtagatgc aatctggcgc ttatcctatc gcaacaatcc cttagcgcct
     4321 aattttggtg taagggttgg cgtgagttta gacatataaa aaaacctgtt tccaaaaaga
     4381 tactcagttt gaaaacaggc ttctataata aattaataat ttgttattgc actttcatca
     4441 gcttcgctaa atatacacct gccgctgttt gcattttaca taaataagtt cctgttggta
     4501 ttgcacttcc atcagtggta tttcccgacc aagtgtaatt aaatgcgccg gcatttgaaa
     4561 ttatatcatc ttcaaacact aattctcctt tcaaattata aattgaaata gccacttctc
     4621 cacttgttgg taattcagca gataaattaa ataaattgcc cgaagggttc ggcgatacta
     4681 acaattcatg ttgcatagct gatgctaatt cttcaatact gatgataatt tcaggtgtgt
     4741 tcaatcttac atctgtatat aagcgatatt ctcccggtaa caaattaatt gttgcgctaa
     4801 tattcgacac gttaatgcta tcccctgaaa aatattcata ccaaaatccg gtatgctgaa
     4861 aattaggatt aacactcgcg ttatacacat caaaattgcc tataaccgta acattcattt
     4921 catcgttatt caaattgatg cgttttcctg aaccggctac atttaatata aagctagtgg
     4981 agctaaatgc aggataagtt gtttttaatt ttattaattc gctccacacc tgattaatgc
     5041 gcaatctact ggcaatatca taataattcc acaaaacagg tttattacaa accctgcaac
     5101 cataatcaat actaatatcg tatcctaatt caccaaattg ccaaatcatt ttcggccccg
     5161 gaatcgtaaa gaaaaacgct cctgccaatt ccattctcga caatgcaacc ggtaaatatt
     5221 ttacattata atccggattt ccgctattac caaaagttaa gtttttatac attaatcgct
     5281 cttcgtcatg actttccata tatcccatta cgtgcggatc actccagcct ctggctacat
     5341 agctcatcca gtgaatatct gaaccggtat aacccattgt gccctgtgca tagttgtaat
     5401 tggaatttcc ccacaacaaa aatccataat cgcttaattc ctcttcttcg gtatttaccg
     5461 caaaatgttc aagaattaat atggcatcct cttcaacggc gtacaataca tcggcaatat
     5521 ctttccaaat tgctatccgt gttgcatcat attgtcccca taatccaaca tcacctaaag
     5581 tatttgtttg tgtatatcct ttcgataaat caaatcgata tccgtcaaca ttaaattcct
     5641 gcaaccaata cgaaaaaata atattgcgca tatttctggt cgcatcactt tcgtgattaa
     5701 aatcataacc aacattatat tcgtgtttag ggactgtatt aaaataaggc gaatttgcag
     5761 ccggtgcgtt tgccagcgga tcccaccaca tttgtgctaa gggactttga ccaaatgcat
     5821 gattcatggc aatatccaaa ataacggcaa tgccatttgc gtgacacgaa tcaataaaaa
     5881 ttttaaatgc ttctcgcgtg ccataatatt tatctaacgc aataaaaaaa cttggattat
     5941 atccccaact ttcattgccc tcaaattcca tcaccggcat aaattcaatc gcattaatgc
     6001 ctagtgcttt taagtaagat aaggtatcaa gtaatgtttg ataatttctt gccgctaaaa
     6061 aatcgcgaac caacaattca taaaccacta aatcctcaac agcaggtggc gtaaactcag
     6121 gaattgccca agtatatgcc ggttgtgctg tttggaacac gctcacaatg cccgaagctt
     6181 ttcctgttgg gtaatctatt aatcccggat aagtaacttc cggtatataa ctatcgttcc
     6241 atgggtccag cactttttca gcgagtggat ctgctaccca taaattacca tcaataaaat
     6301 attggtatgg atattcttta cccgcttcta atcctgttaa tgttacccac catgttgcac
     6361 catctgaatc taaattcata aaactagatt cttgtaacat ccaattgcta tgttcaccta
     6421 cagcaaaaat gtacgattta aatggtgcat acaatacaag tgttaccgta ttatcatcaa
     6481 tataattaat gccttgatgt aaacctgcag gcacatctgc cattatcggg tcaccaataa
     6541 cataataata tgttgaatct actttaatat cgccatctcc ttttgccacc acacgtattt
     6601 cgcctaaacc aaaacttgca gctgtaatat catattctat actggataaa tctgttgcta
     6661 cagcttctgc accatttaca tataaataca tactatcagc aaataccgct gcagctgaaa
     6721 catgaatatt atctcctaaa tcaataataa atgggtctgt tgttggagat gttatggcta
     6781 cgtttagtcc atcttcgtat acattaacaa aaatatctga accatctgta tctcggccta
     6841 caacatcacc ggaagctgtt cgaaaaacaa atgccatttg taaaatggta tcatcttcag
     6901 ttgcgccata aaagtccaga atactggtat actcaatttg atataaatta tcgcctattt
     6961 ccgtcatcag cacctccggg tccggcgttc cccatacacc ttgcgtatat tgccagtcgg
     7021 ttggagagtc gctttcagtg gtaataagcc cggtatgcgc ataaattggg cccggaacat
     7081 ccgctaatgc gccatttccc tgagtggcat caaatataat ggtaaccggc acatctactg
     7141 ttgggaatac cggaatagtt gtaactgcct gagcgctagc taattgtgca attaacaata
     7201 agcctgctaa ttgagtatat cgtctgaaca tacagtaaaa atacaatgat gttgttagaa
     7261 ctcatctcaa attggacagg gattttttat tttaagtaag taaacgcatt tataaatcac
     7321 ttcatgcgca ttagagaaag cattaaaatt tataaaacgt tataaagtag aagttaatta
     7381 ttccttaaat taacagtaaa aactattatt gaatattacc cgcttcatcc atttcaataa
     7441 aaggattgaa ggcaatttcc caataattgt tctcagggtc tgcaacgtaa ccactatatc
     7501 ctccccaaaa aaccttttcg ggtgatttaa ttacggttac atttttagca ataagctcct
     7561 caaacaaagc atttactgct tcaatagaag gcaacaacaa agcagttgta atttgtttaa
     7621 aatttccggc ttctgcttca ataccaatat cctttgctag ttctgtagat ggatacaagc
     7681 ttaatataaa tccattgagc ttaaaaaaca cgatatcacc ctgctccaac attagtgtcc
     7741 agccaaattg attcaaataa aaatttttca tcaaatcaag attgcgaaca ccaatggtga
     7801 gcagagataa tcgttgcgga aacattttaa aataatccct ttttttcttt gtgaataaaa
     7861 gcactgtcta ataaatcgcc atcaaaatct tcaacatcat tatcattaat acgatgataa
     7921 acggcactaa taaaaatggt ttgtcctgca gatgtaactg caataacaaa taaaattcct
     7981 attccggcta aaacaattga aggaattaaa ccaattaatg aaccaagaaa aaataaaggc
     8041 aaaccaataa tcagtatacc taaaatttgc acaatgccaa aactaaatga agagcctaat
     8101 gactcccccc atttttcctt cataatttgt gctgatcttt taaatgcagc aataggagtg
     8161 aggttttcat aagctaatac cggaactaca aaaaatgtgg taattgacca cgccacgcct
     8221 gcaagagcag ctataatttg tccaactttt cccagatttt cttccaataa tcggaaaatc
     8281 aaaccaacag tagcagccat tgccgaccat gctaaaatat cgccgaggcg acttaaacta
     8341 aagtttaaac catccgatac ttttggtaca ccgccctgaa agtaaatgcg tgcgcaatgt
     8401 atcaaaccca tattaaaaaa tacgacaata aaataattga ttaaataaaa tacaaaaagt
     8461 ccgatataag ttatgatatc tcgttcccta actacagctc caaaattgcc ttcattggga
     8521 agtaataaaa tagcaagata agatgcaaac aataataaca atgatgcacc ggataatacc
     8581 ggaaaaagta acaattgttt gtgttttttg ataacggcaa aactgctcat cgacagtttc
     8641 cagccattgg agaggcggtc aagaaatccc atttatgtgt ttatttccca taaatgtaaa
     8701 atttttccga taaaaaaaca attccgggtt atttaagcgt aaagctttgt gctatttttc
     8761 taaaatctga taagtagtcc tcatattttg cgcttgtcat acccaccata atataaaagg
     8821 attcattatc gggatacaac atgacaaaat aaattaattg aggctttttt tctctgtctt
     8881 ttccttcagc aacaatttca taaccactta tattattaat agtaatggga ttaattgtct
     8941 ttattaccgt ctcctcagca taaggtaaac tttttaagcg gctttgagta taagccttgg
     9001 tatcgccaat cgtaacgctg gcaaacgaac tacctaccgt aaataccgcg ttatcagaac
     9061 cctgtgttgg cattttcccg tcttgcgaat acattaatgc gccagaaaca tatccggaat
     9121 atatcattga tgttcctgtt ggatccaatg caaactttac cgcttctttg ccattctcag
     9181 ccgttgtttc attataacgc acacttttca tcgacgataa aataatgtcg cccacagcag
     9241 tctcgttttc aggatacata ccatttacca taactgcttg tttgtcattt ccaaaaatga
     9301 acacttgttt attaaatatt acttcgtttg ctttttgttt aatcaaataa gtaaaagcgg
     9361 taatctcatt aaaaattata gtatcactgt ttattaatat taaacccgct ttcgctaatg
     9421 catctgcagt aaatgaattt acaattacca tataattagt aggtaattct gtaatcataa
     9481 tcgatgaacc tgtagttggt tgctgaaaac cggaaaatgt ttctgcttta tcaaatcctt
     9541 tcggaggcac catactacag tgtgttcccg gaacctggaa aaggctgtca acctgagcgt
     9601 gaacaaaaaa attaatgctc acaaataata tcaacagaat ttttttcatg cgttattttg
     9661 caatttcttt ttgaagaatt tttttatcta aaataaacgg accaaacgga attattgatg
     9721 ccactactgc caataatgtt tttttaaacg gccaatcgtg taccattttt acattaagtc
     9781 cggtaaacat atataaaata aaaagtccgc cgtgaatcca tcccattata tcaaccgcct
     9841 gcggaatatt tgctgcatat tttaaaggca ttgctataaa taataataat aaaaacgaaa
     9901 tgccttcaaa tatggcaact gttctaaatc tgccaatttc tgttgtatac cacttgtttt
     9961 ccatcttgtt gcttttcttt ttagccgcga caaaggtagc cactttttaa ttgaattgtg
    10021 ttaatgacta ttaccccgtt tttttaagtt acttttatac cggaataacc gctactacat
    10081 gttattgata ggtaaaaaac aacgattaaa agcaattcgt tttgtcccgg aaggtgtttt
    10141 tctggacgat ggtgcagaag gtgtattatt gcccagaaag caggtgccgg ccggattgag
    10201 aaccgacgac gaggttgagg tatttatcta taacgatggt gatggtgcac taatggccac
    10261 tacaatggag cccaaagggg ttttggatga tattgttgct ttggaagtaa ttaatgttac
    10321 acctaatggc gcttatttgc aattgggaat accgaaagat gtttttttac acaaaagtca
    10381 aattcgaaat attgccgaac ccggtgatat taaagtggtg aagttaattc tcgattatga
    10441 tggcagactt gctgcaacag aattaattga atcatggctt gataattcgg aattaaccgt
    10501 aattgaaaaa gaacaagttt caatcattat tattcgcgaa accgatttgg gatataatgt
    10561 tgcaataaat aataaacatc ttggcttatt acattacagc gatgtattcc gtcatgtaaa
    10621 accaggcgat gtatttaccg gacatattaa acgtatttta ccggaaaata aattagatgt
    10681 ggcagaagga aaacacggct atcagcgagt tgaagatgag ttggggaaaa ttttgcgact
    10741 gttagacgaa aacgatggct ttttaccatt tcacgacaaa agtgaagccg aagatattta
    10801 tgcttacttc gcgatgagta aaaaaacatt taaaaaagca gtcggcgcat tgtataaaca
    10861 acgaattatt cgtttgagtg aggatggtat tcacaaggtt taatcaacac ttctttttat
    10921 tattattttg tgtgcattac actatcgcgc aacattttca tgtaggctaa cccttttttt
    10981 cttgcaaaat tgatattcct ttccggtatc gacatgggtt cactataatt ttctaaggct
    11041 atatctattt cagcttctct aattaaatga aacatggggt atacagaccg attggtataa
    11101 tttgctgcat cattttcatc actccctgca aataaataat tcggatgaaa tcccgccacc
    11161 tgataaacac cttcaaaacc gctgtttttc attatatctt ctgccattcc taaaaaatcg
    11221 agatacgttc ggaaatctgt taatgattcg ggaacaataa taaaacttgt tgatgttaat
    11281 tcgtgctcgt ctaaataggt gcattcatcc aagataagtt gcaataactt tgctgtttcg
    11341 gcatttgcca ttacgacata tcgaacggcg ttattacgca cgggtgcagc agcaaacgga
    11401 caaaagtgac agccaattac tacctgtttt atccattctt ttgtgtggag gattacatcc
    11461 tcgtcagtta tcaattaata ttcattatcc attcatttag tgcttgataa aacccctaaa
    11521 gcggttatga tatctataag gtgagcaata atatcaatag attaaagata gtggattttt
    11581 aaccactcca acaaaatcaa caaaatcgcc tgttgccaaa gcattacctt tgtctaatga
    11641 aaccggcaac cttatctgat attaaaaaag aactacagct gctcgaccct cttcatgtaa
    11701 cagaactatg tatccgaatg gcccgattta aaaaggagaa taaagaatta ctcacttatc
    11761 tgctttttga gtcatctaat gaacaagctt ttattgaagg tattaaagaa gaaacaattg
    11821 ctttcttcga aacgataaat gatagcaatc tttattttgc caaaaaaagt atccgaaaaa
    11881 ttttgcgcaa tatcaataaa catattcgat attcaggcaa tgcagctact caggtagaac
    11941 tcttactatt cttttgcaaa tcattaaaag aatccggaat tccctatcat accagcgccg
    12001 caatggtgaa tttattcgat gggcaaatta aaaaaatcaa taaagccctg agcactatgc
    12061 acgaagattt acaatatgat tttaaaaaag acctgaaaca attgatataa tcacatattt
    12121 ttactctaaa tccttttctt gagcgccgaa gtaatattaa ttacaccacc atttacgcaa
    12181 ttaaatacgc cctatccagc tacggcatat ttaaaaggat ttctcaatac taaaaatata
    12241 accgcttttc aagccgattt aggtatagag gtaattttaa aggtgtttac taaaaacggc
    12301 ctcaacaaaa tatttgatac cattcagcaa caacatcccg aaaatgcgga tgcattgtcg
    12361 tgtttagtat tgcgaaacga ctacgtggca accatagagc cggttatcct ctttttgcaa
    12421 aataatgagc ctacccttgc acaccgtatt gccgccggcg gttttttgcc ggaaggtccg
    12481 aggtttcagc aatatgataa tttagactgg gctttcggac atatgggggt taccgaaaaa
    12541 gcaaaataca tggctaccat gtatctcgaa gatattgctg atatcatatc aacttttgta
    12601 gaccctcact ttggatttag ccgatatgca gaacgattgg caagatcagc caattcattt
    12661 gatgaattat atcaacaatt acttaatccg ccgacttttt ttgatactat tttattcgaa
    12721 attattcatc agcatattct tcaacatcat cctaaaattt tagccatttc agctccattt
    12781 ccaggtaatg tgtatgcagg atttcgttgt gcgcaatttg taaaacaaca ttttccacaa
    12841 attaaaacag tgttaggagg aggttttgca aatacggaat tgcgcgctgt tgccgatgcg
    12901 cgtgtttttg aattttttga ttttattata ctcgatgatg gtgaagcacc aattgaacaa
    12961 ttggtaaatt ttgtgcgtaa taatattact attgaggaat taaaacgaac atttacttta
    13021 gtaaataaca aagtggttta tatcaacaac agtaaatgta ttgattacaa acaatttgaa
    13081 acaggcactc ccgattacgg cgatttatta ttggataaat atattgcggc tattgaagta
    13141 ataaatccaa tgcatagttt gtggagcaat ggacgatgga ataaacttac catggcacat
    13201 ggttgctatt ggggtaaatg tactttttgc gatatttcat taaattatat tcaagcctat
    13261 gaacctttaa ccgctgctgt tatttgcgac cgcatggaac aaattatggc acaaaccggc
    13321 gaatcgggtt ttcattttgt ggatgaggct gcaccgccgt cattaatgcg cgcagtagct
    13381 ttagaaataa t
//
LOCUS       DAORKW010000036        13311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_71_length_13311_cov_5.893407, whole genome shotgun sequence.
ACCESSION   DAORKW010000036 DAORKW010000000
VERSION     DAORKW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13311
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71_length_13311_cov_5.893407"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PLB46_02865"
     CDS             complement(<1..996)
                     /locus_tag="PLB46_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75485.1"
                     /translation="MRKKIAFVLGVLLLPSAVFAGDGSGKSIDFAGIPVEFILFGLTL
                     LGIALMHKHVLKVALSGLAAVLLLKIGFLQFNLGHHLQEEWSVLLNLLGLLLGFAILA
                     KLFEDSHIPKLLPKVLPHDWKGPFVLLICVFVLSSFLDNIAAAMIGGSIAHVVFRGKV
                     HIGYIAAIVAASNGGGSGSVLGDTTTTMMWIDGVNPLNVIHAYAAAIPAFLFFGIFAA
                     VLQHKYHPMLKGKYESPKIDYKKLVVCFMILAGAITANFLLDFPAAGVWAAIILGALI
                     TQIEWEELQKAVPGTIFLLSLVLMASMMPVNALPTASWETTFVLGFISSVFDNIPL"
     gene            1116..2501
                     /locus_tag="PLB46_02870"
     CDS             1116..2501
                     /locus_tag="PLB46_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HRF75486.1"
                     /translation="MSNLLQTFLQKADTKAFDAEHKRKLLFNIGKYNATVVKGKKQYA
                     DLEYVRKKAKNIKWKVMENLDKYLIEFETNFTANGGKVIWANDEKEAQQEILKILQKK
                     EARMVVKAKSMATEEIHLNEFLGEHNIESVETDLGEYIQQLDGEAPYHIVTPAMHKSK
                     DDVAKLFNEKLGTDLNLTAQELTLVARKNLRQKYVQADVGFSGANFILPDIGGIAVTE
                     NEGNGRLSTSFPKTHIVVVGIEKMLTSVNDLNLFWPLLSTFGTGQKVTVYNTIFTGPK
                     KEVESDGPEEMYVVLLDNGRSKLLAEERQREALYCIRCGSCLNACPVYKNIGGHTYGT
                     TYSGPIGSIISPHFKGMDEFKHLSNASSLCGNCTEVCPMNIELHSLLLYNRNEAVEKG
                     YTTFTERTVWKLWRRSMLSRKSMEKPSPGIKNMALKMLFKNSWGKRREMFTIAPKSFN
                     KMWAEKFPPTE"
     gene            2628..3965
                     /locus_tag="PLB46_02875"
     CDS             2628..3965
                     /locus_tag="PLB46_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF75487.1"
                     /translation="MRILFATLFVISIIEVSAQSQPITFSVYFEKGKADINPADVLTI
                     NDFLALAKDSSYNAVFLKGYADPDGSNTDNMELSEQRVNNVSSMFYGKRYTVESKYYG
                     ETLSINKNKTEAEKAMNRRVDIILWTDFKLAQKNKKPQVFIVSPNRTIEFTAAEGTRI
                     KIPANSLVYENGNTPNGDVKIEITEFYSMLDIVNQQLSTTSNGKVLESAGMINIEASR
                     NDNPLRLKGGTLMDVQFAERAENDGFGLFYGNESARSQVINWTPAANPSAIDKTWNIS
                     GAKLFVTDTIEKWRSKFDYNNLGQRIKVTERWEETKGTSYDTLVLDKTINANKIILQA
                     TRLGWINCDQFYDDKQPTVELYVDAVREYKTDIVLIFTDRKGMILPATAGDGRLVFSN
                     VPKGEKAIITGVGSAEGKLFFAKHDVITGENAIKLDMKETPIATINEQLAVLK"
     gene            4179..4508
                     /locus_tag="PLB46_02880"
     CDS             4179..4508
                     /locus_tag="PLB46_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75488.1"
                     /translation="MLSPENLKKILPGIVTRVAINYPIAYINSVTFIVKNKMVAACFW
                     NEKKNSTEQPSSFALGSDNKSGCFIFHATDEIHNSEIEITGTDERGNDFIVSLPYHHL
                     ISYRLAS"
     gene            4772..5056
                     /locus_tag="PLB46_02885"
     CDS             4772..5056
                     /locus_tag="PLB46_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75489.1"
                     /translation="MFNRKNIEHIIARVVTQSALTLPVAYIQSIVFFNGKTKIADCSW
                     EHFQQNGNLQFYGGDDLTSELLYAPTVVVHAVDMDGTVREFTTAFSNLFA"
     gene            5444..6352
                     /locus_tag="PLB46_02890"
     CDS             5444..6352
                     /locus_tag="PLB46_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRF75490.1"
                     /translation="MKKFNSLSFVATLLLMVAYNTSNAQCATPTGLTATVADATSMNL
                     LWAAVAGADDYTINIQEGPDNPVNYDVTITDVAGTSYLAAGLTASTNYKFKVRTNCGG
                     DHSSWSEYFYFTTGFAGGGGSCDAPTGLAALDITSTSATLTWDVLDAASKYRIRVEDG
                     SGNPVPFTATLGAVTNTTTITGLNPSSNYKFKVRGRCPPGSGSWSDWFDFSTIAGRLG
                     TNEAGLLVYPNPAQNLVTIQIAENELPTQIQLIDLTGKVVYQNEILAGAATTVELSIN
                     DLAAGIYQVVSVAGNQVQTTKLVVTK"
     gene            6658..6801
                     /locus_tag="PLB46_02895"
     CDS             6658..6801
                     /locus_tag="PLB46_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75491.1"
                     /translation="MSRDYIWRLLEGKAGCAVTGEQDVAAPTIFPMYFKAYQGYHGAG
                     ISL"
     gene            6811..8817
                     /locus_tag="PLB46_02900"
     CDS             6811..8817
                     /locus_tag="PLB46_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HRF75492.1"
                     /translation="MDADVQHFSRVMQYDRKNLSNEQLLDLYKRILKPRMIEEKMLNL
                     LRQGKVSKWFSGIGQEAIACAVPYVLEADEYILPMHRNLGVFTGRGIPFGKLFNQWQG
                     KKSGFSKGRERSFHFGTNEYHIVGMISHLGPQLAVADGIALAAKLKKENKVTVVFSGE
                     GGTSEGDFHESLNVAAVWGLPVIFIVENNGYGLSTPTNEQFKCEKIADKGIGYGMKSL
                     TVDGNNYLEMVHVLNECRHYCITENKPVLLECMTFRMRGHEEASGVKYVPKELFDMWG
                     KKDPVVNYEMYLRESGILNDEVNNLFREEIKHEIEYGLEIGFNEPDITPDTNEEVQDV
                     YAPFAQQQVIASGKTSSKKFINAISDALSLAMEKYPELVIMGQDIAEYGGAFKVTEGF
                     VDKFGKERIRNTPICESAILGAGCGMSIKKMKTVIEMQFADFVTSGFNPIVNNMAKMY
                     YRWGQNINSVVRMPTGAGVGAGPFHSQSNEAWFFHTPGLKVVYPSNAHDAKGLLLSSI
                     EDPNPVMFFEHKGLYRSITEDIADNYYTEPIGKAKLVTSGNEVSVITYGMGVHWAKKV
                     CAENNIDADILDLRTLLPLDYDAIQATVKNTGKVLVLHEDTLIGGIGGEIAAYISENL
                     FQYLDAPVKRCASLDTPIPFSIELEQNFLANSRLKEKLMELMAY"
     gene            8895..10091
                     /locus_tag="PLB46_02905"
     CDS             8895..10091
                     /locus_tag="PLB46_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75493.1"
                     /translation="MKLKLIASIFPFIILLCCKQWVAAQTYTPGVSYFGAHGYTEYIP
                     GSLPIIISAPHGGYLEPEVIPNRTCFDAVYATDAYTQELIRDIQNSIYAEFGCYAHVV
                     INLLDRKKLDANRNLAEGACGNDSAKQAWNDFNDFLDIAENTVMEQYAKGLYIDLHGH
                     GNPKQRLEIGYLLYDDELQLSDTELNTTTYINYSSIQHLVETNASGFTHAELLRGAIS
                     FGTLMEEAGYASVPSAADPFPLTGDNYYSGGYNTVERSSYSGGTIDGFQIECNYTGVR
                     DNSENREKFADSIVSVLRAYLETHYFIDNELSDCSKFVSINEHQHDLVKVAIYPNPAS
                     QVLQINSDKIISSISIYTITGTKLITLNEATDFHQISISALKPGCYVMQLLIEGEVVV
                     RNFLKL"
     gene            complement(10459..11352)
                     /locus_tag="PLB46_02910"
     CDS             complement(10459..11352)
                     /locus_tag="PLB46_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75494.1"
                     /translation="MKPQFPYKKLAGYSIFATAFLSTKSSESQIVYTDIDDVTLSADN
                     EFYNIDLDYDGLYDFKLQKISGSFYSYWSEHLSYADYIFIAPLQSGNKIAGLKSVIDP
                     SYGGFTQYFPFALNSGALINEDLTFQNNNFEILAYKIRNDDGGGGPSGGEWFYNNADQ
                     YLGVMFVDTLDCKHYGWIRCEAFGGADSIVIKEFACVIPCELGVYAGTYLTDTSDIAI
                     NSLQNFKPTIYNYSNVLTININANLVGSNYTITSADGKLIGSGNLNQSNNTINLTCAP
                     GIFIVTVKNAGSSYSQKIIVH"
     gene            11447..11875
                     /locus_tag="PLB46_02915"
     CDS             11447..11875
                     /locus_tag="PLB46_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRF75495.1"
                     /translation="MPIHLQHLESETNEKTRLRGYDYYCEGRVKSVFNINGIEWMAVV
                     QGYDDHVVQLNITNDSVLRHECNCSDAEDPCKHVIAVLYYIRNEAHKLKKTDATGYEE
                     IKENISYLPPHVLRYLILKYATISKEFRKFLLDYTKDGKK"
     gene            11995..12375
                     /locus_tag="PLB46_02920"
     CDS             11995..12375
                     /locus_tag="PLB46_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75496.1"
                     /translation="MNTKKHIGDLHPEVKAWMSYVSYVKDELKVHNLRLEEIISKNTG
                     REVMAHTEHFQNQFIRQNEACDELMHDLHKVEKSYSDLAANNPAAMHVAIADETGLRD
                     RVDTHRHIFEDLTHEFRAFLSKHM"
     gene            12548..>13311
                     /locus_tag="PLB46_02925"
     CDS             12548..>13311
                     /locus_tag="PLB46_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75497.1"
                     /translation="MKRILLLFFGLSVFTSAFAQSTWESVYAVLQTNCGSCHVAGHTS
                     GLDLSASIGEVYDNLYDLTPTNATSASKGYKRVFPGEPYKSFLFSKVNNGLAWDVNLE
                     AGEGEACPQGAAPLNNKDIELIRQWIMYGAFESDFAVDIPLINEFYDNGGIQSVPLPP
                     APPAEGEGFQVHFGPFFLWPEEEHEYFTKFFTQIPSDLEINRVDTYMGDYSHHFITYR
                     FTEPFWEEFTPYGLHDGPDFAGIDLVTANQYPQNIV"
BASE COUNT         4398 a         2175 c         2673 g         4065 t
ORIGIN      
        1 gagtggaata ttgtcgaaaa cgctggaaat gaagcccagc acaaatgtag tttcccagga
       61 agcggttggc aaagcattaa ccggcatcat agaggccata agcactaaac tcaacaggaa
      121 tatggttcca ggaacggcct tttgtaattc ttcccattca atttgggtaa ttaacgcacc
      181 caaaataatt gctgcccaaa ccccggcagc aggaaaatct aataaaaaat tagcagtaat
      241 agctcctgcc aaaatcatga aacaaaccac cagttttttg taatcaattt ttggcgattc
      301 gtatttcccc ttgagcattg ggtgatattt atgttgcagc acagctgcga aaatgccaaa
      361 aaataaaaat gccggaattg ctgcggcata ggcatgaatt acgttcaatg gatttacgcc
      421 atctatccac atcatagtag ttgtggtgtc gcccaacacg gaaccagaac ctccgccgtt
      481 acttgcagca acaatagctg caatatatcc gatatggact ttacctctga acaccacatg
      541 ggctatactg cctccaatca tggctgcagc tatattatct agaaaggaag aaagtacaaa
      601 cacacaaata agtaatacaa aaggtccttt ccagtcgtga ggaagcacct tgggaagtag
      661 tttgggaatg tgagaatctt caaacaattt ggctaaaatg gcaaagccta aaagcaaacc
      721 caataaattt agcagcaccg accattcttc ctgtaaatga tgacctaaat tgaattgtaa
      781 aaacccaatt ttaagcaaaa gcaccgcagc aagtccgctc aaagccactt ttagcacatg
      841 tttgtgcata agtgcaatgc ccaataaggt gagaccaaat aaaataaatt ccaccgggat
      901 accggcaaag tcgatacttt tacccgaacc atcgccggca aatacagccg aaggaagcag
      961 taacacccct aaaacaaaag caattttttt acgcatataa gaaacaatgg aaaatcaaaa
     1021 atagaactat tttgtgcata acggattttg gttgtggaaa gatattaatg gatatctata
     1081 catatgtagt atttttgtgc agttgcgcac aataaatgtc gaacttacta caaacatttt
     1141 tacaaaaagc agacaccaaa gctttcgatg ctgaacataa acgcaagttg ttgttcaata
     1201 ttggaaaata taatgccacg gtggtgaagg gcaaaaaaca atatgccgac ttagaatacg
     1261 ttcgcaaaaa agccaaaaat attaagtgga aggtgatgga aaatctcgat aaatatctta
     1321 tcgaatttga aaccaacttt acagcaaacg gaggaaaagt aatttgggct aacgatgaaa
     1381 aagaagctca acaggaaatt cttaaaattc ttcagaagaa agaagccaga atggtagtta
     1441 aagccaaaag tatggctact gaagaaattc atctcaacga atttttaggg gaacacaata
     1501 ttgaaagtgt tgaaaccgat ttaggggaat acattcagca gctggatgga gaagcaccat
     1561 atcatattgt tacaccggca atgcataaaa gtaaagatga tgttgccaaa ttgtttaacg
     1621 agaaactagg aacagacctc aatcttactg cacaggaatt aaccctggtt gccagaaaaa
     1681 acttgcgcca aaaatatgtt caggcagatg taggttttag tggagccaat tttattttac
     1741 ctgatattgg cggtatagca gttaccgaaa atgaaggtaa tggtcgcctt agcaccagtt
     1801 ttcctaaaac acatattgtt gttgtaggta ttgaaaaaat gcttaccagt gtaaacgatt
     1861 taaatttatt ttggccattg ttatccacgt ttggaactgg tcaaaaagta actgtatata
     1921 ataccatttt caccggaccc aaaaaagaag ttgagtctga tggtcctgaa gaaatgtatg
     1981 ttgtgttgtt ggacaatggc cgctcgaaat tattggcaga agaaagacaa cgtgaagcat
     2041 tatattgtat cagatgcggc tcatgtttaa atgcatgtcc ggtatacaaa aatattggag
     2101 gccatactta tggcaccact tactccggac caatcggttc gataatatct ccacatttta
     2161 aggggatgga cgaatttaaa cacctgagca atgcatccag tttatgcggc aattgcaccg
     2221 aggtttgtcc aatgaatatt gagttacaca gtttattatt atataataga aacgaagccg
     2281 ttgaaaaagg gtatactaca tttaccgaaa gaacagtttg gaaactatgg cgacgttcaa
     2341 tgctgagcag aaaaagtatg gaaaaaccat cgcctggcat taaaaatatg gcactcaaaa
     2401 tgctcttcaa aaattcatgg ggaaaacgcc gtgaaatgtt tactatagcg cctaaatcat
     2461 ttaataaaat gtgggccgaa aaatttccac caactgaata aaaccttaag gtgggttaag
     2521 ttgcaaatgg cgctttccgg ctttttgatt tgttgcaaga tggcccaata atccacatca
     2581 aacaatattt tttaacgtaa tggcgtttgt tacccaaact cacacctatg cgtatattat
     2641 ttgctaccct gtttgtaatt agtataattg aagtatctgc gcaatcacaa cctatcactt
     2701 tttcggtgta ttttgaaaag ggtaaagccg atattaatcc ggctgatgta ttaaccataa
     2761 atgatttttt ggcactggcg aaggactctt cgtataatgc tgtttttttg aaaggatatg
     2821 ctgaccctga tggatctaat actgacaaca tggagttgtc ggagcagcgt gtaaataatg
     2881 taagcagtat gttttatggt aaacgttata ctgtagaatc gaaatattat ggcgaaacgt
     2941 tatccataaa taaaaataaa acagaagctg aaaaagcaat gaaccgcagg gtagatatta
     3001 ttttatggac agattttaaa cttgcacaaa aaaataaaaa accacaggta tttattgtat
     3061 ctcccaaccg aacaattgaa tttactgcag ctgaaggtac cagaataaaa atacctgcta
     3121 atagtttagt atatgaaaac ggcaatacac ctaatggcga tgtaaaaatt gaaatcacag
     3181 aattttattc aatgttagat attgttaatc agcagctttc tactacttca aacgggaaag
     3241 tgttggagtc tgccggcatg attaatattg aggcgtcgag aaatgacaat cctttgcgtt
     3301 tgaaaggcgg aactttaatg gatgttcaat ttgctgaacg agcagaaaac gacggattcg
     3361 gattatttta cggaaatgaa agtgccagat cacaagtaat taactggact cctgctgcaa
     3421 atccttctgc aattgataaa acgtggaata tttcaggagc taaattattt gtaacagata
     3481 ccatagaaaa atggaggagt aaattcgatt ataataattt aggtcagcgc ataaaagtta
     3541 ccgaacgatg ggaagaaaca aaaggcactt cttatgacac tttagtttta gataaaacaa
     3601 ttaatgccaa taaaattatt ttgcaggcta cgcgattagg ctggataaat tgtgatcaat
     3661 tttatgacga caagcagcca acagttgaat tatatgttga tgccgttcgc gaatataaaa
     3721 ccgatatagt attaatattt acagacagaa aaggtatgat acttcccgcc actgcgggtg
     3781 atggaagatt agtttttagt aatgttccta agggagaaaa agcgataatt accggtgtgg
     3841 gttctgcaga agggaagttg ttttttgcca aacatgatgt aattacaggt gaaaatgcaa
     3901 taaaactgga tatgaaagaa accccaattg caacaattaa tgaacaattg gctgttttaa
     3961 aataacattt tcatgcctga tttatgaaaa ggccggaaat tcgtcccgga attatttaca
     4021 ggttacattg cctgaaaccc ttgtcgggat tgaatttagt gtaattggta tttgaacggg
     4081 caagacattt taatgcaaat gagactggaa gtgagacaaa tgtgtatttg agagttgtat
     4141 gtttatacat gaattttgga tcatcaaaac caatagttat gttgagtcca gaaaatttaa
     4201 aaaagatact tccgggaatt gtaacaagag ttgcaattaa ttatccaata gcctatatca
     4261 attctgtaac ctttattgta aaaaataaaa tggtagcagc ctgtttttgg aacgagaaaa
     4321 aaaacagcac tgaacagcct tcgtcatttg ctttgggttc agataataaa agtggttgtt
     4381 ttatttttca cgctactgat gaaattcaca atagtgaaat tgaaattacc ggaactgatg
     4441 aacggggaaa cgactttata gtttctttac cttatcacca tctgattagt taccgattag
     4501 cttcttaaac tacaccactc ttaacagcac tcttggttat gtttaaataa ttctgcgaaa
     4561 agcatttgca cctgccatat tactatagac gatacgacac tgatttttgt cgtaacaaaa
     4621 aattgttgaa aagcgcctgt ttataagggt tgctgacatt cacctgaggc aaattgcctg
     4681 ccaccaataa aaaactttac tgatacgctt caatatttgt atttgaagtt gcgttataag
     4741 gttagcattt ttgaccttgt aatcagcaca catgttcaac cggaaaaata tagaacacat
     4801 aatagcaagg gtagttactc aaagcgccct tacattgccg gtggcttata ttcaatctat
     4861 tgtatttttt aatggtaaaa caaaaattgc agattgttcc tgggagcatt ttcagcaaaa
     4921 cgggaatttg caattttatg gtggtgatga tttaaccagt gaattattat acgcgccaac
     4981 agtagttgta catgctgtag atatggatgg aacagtgcgt gaatttacaa cagcatttag
     5041 caatttattt gcgtaaaaca gtttttttca tacttggtag ataaaggggt caggagagga
     5101 gcctgattcc tttttttatg ccttttttta agcaaaattg ccgaaacagc ttattatgtg
     5161 tcgctacata tatttttgct aagtttcttc aatcccttgt ttttgagcta tttacattat
     5221 aatcttcact ctgttaccca tgttcttcgg gtaaaaatat atgtatatac agtggaaatg
     5281 taaaaattct gtattggtta gttgtatttt atcgccacac ttttgtatcg tgattagtaa
     5341 acaatattca ttattactaa ttaccaggta acaatttatc cggccgataa atatactcaa
     5401 aaggacatgc acaatttaat tcacttttta aattgaaatt attatgaaaa aattcaactc
     5461 attatcgttt gttgcaacat tattattaat ggttgcttac aacacttcta atgcacaatg
     5521 tgcaacacca acaggattaa cggctacagt ggcagatgct acatcaatga atttattatg
     5581 ggctgctgtt gcaggtgcag atgattatac aattaatatt caggaaggtc ctgataatcc
     5641 ggtaaattat gatgtaacaa ttactgatgt tgccggaact tcttatttag ctgcgggtct
     5701 gactgccagc acaaattata aatttaaagt aagaacaaat tgtggtggtg atcattcatc
     5761 atggtctgaa tatttttatt ttacaacagg ttttgccgga ggtggtggtt catgtgatgc
     5821 tcctacagga ttagcggcgc ttgatataac ttcaacttct gctacattaa cctgggatgt
     5881 tttagatgca gccagcaaat atagaattcg tgttgaagac ggaagtggca atccggttcc
     5941 atttactgct acattgggag cagtaaccaa taccacaaca ataacaggat taaatccttc
     6001 atccaactac aaatttaaag ttcgtggtcg ttgcccacca ggttcaggaa gctggagcga
     6061 ttggtttgat ttttctacca ttgctggtcg acttggaaca aacgaagctg ggttattagt
     6121 atacccaaat cctgctcaaa atttagttac tattcaaatt gctgaaaatg aattgcctac
     6181 ccaaattcaa ctgattgatc ttaccggaaa agtagtttac caaaatgaaa ttttagcagg
     6241 tgcagcaaca actgttgaat tatctattaa cgatttagct gccggtattt atcaggtagt
     6301 gagtgttgct ggtaatcagg ttcaaacaac aaagttggtt gtgactaaat aagtttttac
     6361 gacgtatata tgaaaggaag gggtacagtg aaagctgtgc ccttttttgt tttatttttt
     6421 ttaaaaacca cacaaaaaat taattcgcat gttaccattc gttttttcta atcaaaaatt
     6481 gtgatgttat ttgatgttat ttgttataat taaattggca aaccggatgc atttttaatc
     6541 accattaaaa ttggcattac cactgtacaa attagcccgg attggaccgg aaacaaacag
     6601 ccaatgtgct gatgcgcgca gtgtccgctg aggtggatgc taattaaaca tccgaaattg
     6661 agccgagatt acatttggcg tttgttggag gggaaagccg gttgtgcagt aactggggag
     6721 caggatgttg ctgctccaac tatttttccg atgtatttta aggcttatca gggttatcat
     6781 ggagcgggga tttcgttgta actttgcgcc atggatgcgg atgtacaaca ttttagtcgc
     6841 gtaatgcaat acgatagaaa aaatttgagt aacgaacagt tgctcgattt atataagcgg
     6901 attttgaaac cgcgcatgat tgaggaaaaa atgctaaatt tattgcgtca gggcaaggtg
     6961 agcaagtggt ttagcggcat tggacaagag gcgattgcct gtgccgtgcc gtatgtgttg
     7021 gaagcggacg aatatatttt gccgatgcat cgcaatttgg gagtatttac cggtcgcggt
     7081 attccgtttg gcaaattgtt taatcaatgg caaggtaaaa aaagcggatt tagtaaaggt
     7141 cgcgagcgca gttttcattt tgggacaaat gaatatcata ttgtaggtat gatttcgcac
     7201 ctggggccgc aattagcggt tgccgatggt attgcacttg ctgcgaaatt gaaaaaagaa
     7261 aataaggtta cggttgtttt ttcgggagaa ggtggaacaa gtgaaggtga ttttcacgaa
     7321 tcgttaaacg ttgctgctgt ttggggttta ccggtaattt ttatagttga aaataatggt
     7381 tatggtttat cgacaccaac caatgaacaa tttaaatgcg aaaaaattgc agataaaggc
     7441 attggttatg gtatgaaatc gcttacggta gatggcaata attatttgga aatggtgcat
     7501 gtattaaatg aatgcaggca ttattgtata acagaaaaca aaccggtatt gctggagtgt
     7561 atgacattta gaatgcgcgg tcacgaagaa gcaagtggtg taaaatatgt gcccaaagaa
     7621 ttgtttgaca tgtggggcaa aaaagatcct gtcgtaaatt atgaaatgta tttgcgtgaa
     7681 tcaggtattt taaatgatga agtaaataat ttattcagag aagaaataaa acacgaaatt
     7741 gagtacgggt tagaaattgg ttttaacgaa ccggatatta cacctgatac caatgaagaa
     7801 gtgcaagatg tgtatgcacc gtttgcacaa caacaagtta ttgcatcggg aaaaacatct
     7861 tcgaaaaaat ttattaatgc gataagtgat gcgttgagtt tagcaatgga aaaatatcct
     7921 gagttagtaa ttatgggtca ggatattgca gagtacggtg gtgcatttaa agtgactgaa
     7981 ggatttgtag ataaatttgg caaagagcgc attcgcaata caccaatttg tgaatcggcc
     8041 attttaggtg caggatgtgg tatgtcgatt aaaaaaatga aaacggtaat tgaaatgcag
     8101 tttgccgatt ttgtgacgag tggatttaat ccgattgtaa acaatatggc aaaaatgtat
     8161 tatcgctggg gtcaaaatat aaactcggtg gtgcgcatgc caaccggtgc cggtgttggt
     8221 gcaggtccgt ttcactcaca aagcaatgaa gcgtggtttt ttcatacacc tggattaaaa
     8281 gtggtgtatc caagtaatgc acatgatgca aaagggttat tattatcttc tattgaagac
     8341 ccgaatccag taatgttttt tgaacataaa ggattatatc gcagcattac agaagatatt
     8401 gctgataatt attataccga acctattggg aaagcaaaat tggtgacatc aggaaatgag
     8461 gtgtcagtaa ttacttatgg tatgggcgtg cattgggcga aaaaagtatg tgctgaaaat
     8521 aatattgatg ctgatatttt agatttacgc accttgttgc cgctggatta tgatgctata
     8581 caagcaacag ttaaaaatac cggtaaagta ttggtattac atgaagatac cttgattggt
     8641 ggtattgggg gagaaattgc tgcatatatt tccgaaaatt tattccaata tttagacgca
     8701 ccggttaaac gttgtgcgag tttagatacg cctattccat ttagtattga actggagcaa
     8761 aattttctgg ccaatagcag gttgaaggaa aaattgatgg agttgatggc gtattaattt
     8821 ttatattaat tacttaacgt attggatatt gcagttaagt ttgtttttat tttgccaaca
     8881 ccaataacat gcaaatgaaa ctgaaattaa tcgctagtat ttttccattt ataatactgc
     8941 tttgttgtaa acaatgggtt gctgcacaaa cttacacacc cggtgtatct tattttgggg
     9001 cacatggtta tacggaatac attcccggtt cactaccaat aattattagt gcaccacatg
     9061 gcggttatct ggagccggag gttattccaa acagaacttg ttttgatgcg gtgtatgcaa
     9121 cagatgcgta tacgcaagaa ttaattcgcg atattcaaaa ttcgatatat gctgaatttg
     9181 gttgttatgc gcatgtggta attaatttat tagatagaaa aaaactggat gccaacagaa
     9241 atttagcgga aggtgcttgt ggaaatgatt cagccaaaca agcatggaac gattttaacg
     9301 actttcttga tattgccgaa aatactgtga tggaacaata tgcaaaagga ttgtacatcg
     9361 atttacatgg tcatggcaat cctaagcagc gattagaaat tggttattta ttatatgatg
     9421 atgagttgca attaagcgat acggaattaa atacaactac ttatattaat tatagcagca
     9481 ttcaacattt agtagaaaca aatgcatcag gtttcacgca tgctgaatta ttaagaggtg
     9541 caattagttt tggcacttta atggaggaag caggatatgc atcggtgcct tctgctgcag
     9601 acccatttcc tttgacaggc gataattatt acagcggtgg atataatacg gttgagcgtt
     9661 catcgtattc gggtggaaca attgatgggt ttcaaattga atgtaattat accggtgtta
     9721 gagataattc agaaaaccgg gaaaaatttg cagacagtat agtttcggtt ttaagggcct
     9781 acctggaaac acattatttt attgataatg aattgagtga ttgcagcaaa tttgtgagta
     9841 ttaatgaaca tcagcatgat ttggtaaaag ttgcaattta ccccaacccg gcatcacagg
     9901 tgttacaaat taattccgat aaaataattt catcaataag tatttatact attaccggga
     9961 caaaactgat aacattaaat gaagcaacag attttcatca aatatcaatt tcggcactta
    10021 agcccggttg ttatgttatg cagttgttaa ttgaaggaga agtggttgtt cgtaattttt
    10081 taaagctgta aagtcgcttt ggataagttc atttgaatgt ttttaattgc ttcaattttg
    10141 taattcccat aactataaat attatcccta aaataaaata cagacataat gaaactatac
    10201 caactacgtc cattgaaatt gctgtagggt caggtgtgtt taacttcttt aatattcgaa
    10261 aaaagttcac aattctaaga attgccaata atagcagcac tattcctgca atcaatatta
    10321 ggattgctaa atttcgtttt gacataggta taaaattcct gtgtttaaaa atacatgttt
    10381 tcaattacaa gcatcaattc cttgagatat tactcatgta aagaatttaa ttatggtatt
    10441 tatttataaa agaaaagatt aatgaacgat tattttttgc gaataactac tacctgcatt
    10501 ttttactgta acaataaaaa tgcccggagc acaagttaag ttaatcgtgt tatttgattg
    10561 atttaaattt cctgaaccaa tcaatttgcc atctgctgat gtaattgtat agttactgcc
    10621 aacaagatta gcattaatat ttattgttaa aacattacta taattataaa tagttggttt
    10681 aaaattttgc agactgttta tggcaatgtc agaggtgtca gtaaggtatg tgcccgcgta
    10741 aactccgagt tcacatggaa taacacatgc aaattcttta ataactattg aatctgcacc
    10801 accaaatgct tcacatctta tccatccata gtgtttacaa tccaaggtgt caacaaacat
    10861 aacccccaaa tattggtctg cgttattata gaaccattct cctccgcttg gaccaccacc
    10921 gccatcgtca tttcttattt tataggctaa aatttcaaaa ttattattct gaaaagtcaa
    10981 atcttcatta attaaagcgc cactgttaag tgcaaaggga aaatattgtg taaatccgcc
    11041 ataggatggg tcaataacac tttttaatcc tgctatttta ttgccgcttt gcaagggagc
    11101 tataaaaata taatctgcat aggagaggtg ttcactccaa taggaataaa atgaaccgct
    11161 aattttttgt aatttaaaat catataatcc gtcgtaatct aaatctatat tataaaattc
    11221 attatctgct gataaggtaa catcatcaat atcggtataa actatttggg attcactact
    11281 ttttgttgac aagaatgcag tagcaaatat cgaataaccg gcaagctttt tatatgggaa
    11341 ttggggcttc atacaacttt tattatttac actggattag aatttatagt taaaaatacc
    11401 tattttcaat tataagttac ctgctcgttg atgtaatttt gcacttgtgc caatacatct
    11461 gcaacattta gaatcagaaa caaacgaaaa aacccgtctg cgcggttacg attattattg
    11521 cgaaggccgg gtaaaatcgg tgtttaatat taatggtatt gaatggatgg cggtagtgca
    11581 gggttacgac gaccatgtgg tgcagttgaa tattacgaat gactctgtat taaggcatga
    11641 atgtaattgc agtgatgcgg aggatccatg taaacatgta attgcggtgt tgtattatat
    11701 ccgcaacgaa gcacataaac tcaaaaaaac cgatgctacc ggttacgaag aaataaaaga
    11761 aaatatcagt tatctgccac cgcatgtgct gcggtatttg atattaaaat atgctactat
    11821 tagcaaggag tttaggaagt ttttgctcga ctacactaaa gatggcaaaa agtaattttg
    11881 aaatagtgct cggctgctaa atcgaaatgg tgataaatgt tgtacttgtt gctgatttct
    11941 attatctgaa gggtaatggc taaagcccat ctttgaatat aaaacttaaa cgttatgaat
    12001 accaaaaaac atatcggcga tcttcaccct gaagtgaaag cctggatgag ttatgtgagt
    12061 tatgtgaaag acgaactcaa agtgcacaat ttgcgtctcg aagaaatcat tagcaaaaac
    12121 accggacgtg aagtaatggc ccataccgaa cattttcaaa accagtttat ccgtcagaat
    12181 gaggcttgtg atgaactgat gcacgatttg cataaagtag aaaaatctta cagcgactta
    12241 gcagccaata acccagctgc catgcacgtg gctattgctg atgaaacagg cttacgcgac
    12301 agagtggata cacacaggca tatatttgaa gacctgactc atgagtttag agcatttttg
    12361 tcgaaacaca tgtaagttat taataatgaa tggtttagca caatagctgc taccattttt
    12421 atgctggtga attaacattt aaccatgtta tggttatgtt aaacaaatgc ttaaacagtg
    12481 agatttttta ttgaaatgcc ttaagctaga ggtaaattcc ttaatttcgt tagctcaaag
    12541 gcattttatg aaaagaatct tattattatt ttttggttta tcagtattta cttctgcatt
    12601 tgcacaatct acctgggaat cggtatatgc tgttttgcaa actaattgcg gcagttgtca
    12661 cgttgccggg cataccagcg gtttggattt aagtgcatca attggtgagg tgtatgataa
    12721 cttatatgac cttacaccaa caaatgcgac ttcagcatcg aaaggttaca aacgtgtatt
    12781 tcccggtgaa ccttataaaa gttttttgtt cagtaaagtt aataacggct tagcctggga
    12841 tgtaaatctc gaagccggtg aaggtgaagc atgcccacag ggtgctgctc cgttaaataa
    12901 taaggatatt gaattaattc gtcaatggat tatgtatggt gcttttgaaa gtgattttgc
    12961 ggttgatatt ccattaatca atgagtttta tgataatggt ggtattcaaa gtgtgccact
    13021 acctcctgca ccacctgctg aaggtgaagg atttcaagtg catttcggtc cgttcttttt
    13081 atggcctgaa gaagaacacg aatatttcac taaatttttc acacaaattc caagtgattt
    13141 agaaattaat cgcgtagata cttatatggg agattactcc caccatttta tcacttaccg
    13201 attcactgaa ccattctggg aagaatttac accatacggt ttacatgatg gtcccgattt
    13261 tgcaggaatt gatttagtaa ctgcaaatca gtacccgcaa aatattgtat t
//
LOCUS       DAORKW010000037        13286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_72_length_13286_cov_5.366488, whole genome shotgun sequence.
ACCESSION   DAORKW010000037 DAORKW010000000
VERSION     DAORKW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13286
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72_length_13286_cov_5.366488"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PLB46_02930"
     CDS             complement(<1..468)
                     /locus_tag="PLB46_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75498.1"
                     /translation="MWLRFKWLSVLMFVAAVTSAQHINIDQFNNEVIKKELHQAAYKT
                     SAFDAAETGNYDIFYHKCNWNIDPDTLMINGSVETHFTLTQNATSIFYDAADNLIIDS
                     VWLNEINIDFNRITNAVEILFGEELSSGENLSTTVFYRGSPASTGFGSFVKGTN"
     gene            complement(468..875)
                     /locus_tag="PLB46_02935"
     CDS             complement(468..875)
                     /locus_tag="PLB46_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75499.1"
                     /translation="MIDFLKRFIPFSAVVALALFGLGKLEMFSGAMDFVWICFVLFFV
                     LAIVIYYFSARTMRQKFSNFMGAFFVSIFAKLILSAIVVMVYKSTNDVGGIAFIAPFA
                     LIYFSFLIFETLELVKLSKALGNNAPPKNPIVK"
     gene            complement(879..1121)
                     /locus_tag="PLB46_02940"
     CDS             complement(879..1121)
                     /locus_tag="PLB46_02940"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRF75500.1"
                     /translation="MPQRNTKKPQFDSYFKYSGMAFQLFAGIFLGVWGGMKLDEVLHT
                     KPWFTVILSLFGIAAGMYAVLKDFILPNKNKKNNKK"
     gene            complement(1087..1497)
                     /locus_tag="PLB46_02945"
     CDS             complement(1087..1497)
                     /locus_tag="PLB46_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRF75501.1"
                     /translation="MFGNKQSNPQSAPGSFISNRINHGTTFEGSITSDGDIRIEGIIR
                     GTLYTKAKVAVGQTGVIEGDVHCKSADVEGKIIGDLEVSEVLTLKSTAIVEGNIYTAK
                     IVIENGAHFDGICNMGTKERKLTNASAQYEEATV"
     gene            complement(1512..2324)
                     /locus_tag="PLB46_02950"
     CDS             complement(1512..2324)
                     /locus_tag="PLB46_02950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRF75502.1"
                     /translation="MHDKTFEVQASLRLSPLNVIILSSTLFVVIGFLVYLAIVFTPLK
                     NLVVGYSEVSTTRQIAENNKITDSMLTALKSYEVYFANLQKRLNGEIDTLPPIDSLSK
                     QDYSGIKVGEASEIDKAMREQVAREDLFNLSLADPRDATGEQNLESLHFFPPIKGTVT
                     SSFQTREGHYGIDVVAPENTPIKACLDGVVIDSYWNLESGNVIIIRHDHGLISIYKHN
                     SQILKRQGNIIRAGDVIAIIGNTGEMSTGPHLHFELWHEKTPLNPKDFINFN"
     gene            2479..5085
                     /locus_tag="PLB46_02955"
     CDS             2479..5085
                     /locus_tag="PLB46_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF75503.1"
                     /translation="MKRNSVIFTLTIIVLFFVSACSPGKNADKSFIGRSVDDLIARDN
                     GYFNARLIMKETEHTLWTDQVDNYDETLPIFKYGTQEQATSIQPSMDEVIKKTSFVIQ
                     MHKKSKWVDDSFFLIGKSQFYKRNYDEALTSFQYIISEYSNLIEKQSKAKRVAEDEDG
                     ELTFFEKLKHQPVSSEAGIWVARTLVEKKQYSDAHTVISVLKSRENFPKWLIGELYAV
                     EADLYMKEGQEANAIEPLINALKNTNDKALTNRYNFILAQLYAQQKNIDKALATYQLV
                     LDNKPVYDMEFYAQLNIAKLSLANFGVSGGESKEELMALLKDEKYKDFYGLIYYTLAD
                     IALANKDEVTGVDYLNKSLRSTNDSKQKGQSYLRLADLYYADLDYKAAYAYYDSTINQ
                     IQREHERYTEVRGLRDNLKLLVDELTIIETEKKLQYWAGLSEKELEKELAKLIPEKEE
                     TDSLATDVLNPNNVKPLAGNPTEGDFYFYNTNLRSRGFSEFKKNWGTRKLEDNWRRSE
                     KGTFSEEGGTEPIVGEEKTIDLNAKNVTTEKIIASLPKTPDQIAVSNAKIAGALYRAG
                     IVYKDNFRNKTNAIAMFEENVNNYPANTFEVQSLYYLYRLTLNPQQDKYKNLLLSKYP
                     ETDYAKIISDPDYFKREEDRKHAIESYYTVTYDALQAKNYAEVKSRALAADSLFKPNT
                     LKPKFDLLTALAEVDSIETFKNALQKIALKYPENEVGIRAQEMLDYLRRGSVMEDAGK
                     TIPVAAYTMNAEEEHFFMFVMNSTGKNATTMKSNIATFNGANFSTDNLKISSLLLGKD
                     NSLILVKSFNNVEKAMVYYFAIKDNLGVFKDLTKETFTPFIISKSNYVQFFKVKDVEA
                     YDVYFQDNYLKE"
     gene            5096..7384
                     /locus_tag="PLB46_02960"
     CDS             5096..7384
                     /locus_tag="PLB46_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRF75504.1"
                     /translation="MSIKEQFAVYKQQIKWFWRIFFIGAGVVTLFIILLSFGVFGKLP
                     SFEELENPRSNLSTQLISSDGELLGRFYRENRTNATYKELPPHLIDALVSTEDRRYYE
                     HSGIDAKSVMRVIFKTLILQQSESGGGSTLTQQLAKNLYGRPTVSNKFELAVTKLKEW
                     VTAAKLERRYTKEEIIVMYLNTIPYIYDASGIKTASKTFFNKSVSDLTVEEAAVLVGM
                     VQNPAFYNPVRFPERTLERRNIVLHEMHEAGKLSDKDYEATKAKPLVLDFKRQDHKSG
                     NGTYFREYLRQWLADWVEENEKPDGTKWDIYKDGLKVYTTIDSRMQSYAEAASSQWLT
                     KLQKQFDGHWKGNKYEPWAYPYSGRTNPELIEKSIKQTERYRKMKKAGASEDEITKAF
                     HTPDTMTVFSWYKEGYDLDTVMTPYDSIKYVKKILHPGFMAMDPHTGEIKAWVGGVDM
                     RYFQLDHVKPAQGRQVGSTFKPFVYTAAIADKGFSPCYQIPNLPVTFEKGQKWSLISD
                     WTPKNSDGSYGGMLTLSQGLGKSVNTVTAYLLREVGIQPVIDLAHDMGIESEIPFQPS
                     ICLGTPEVTVYEMTAAYSAYANKGVAVRPVFVTRIEDKNGNIIAEFPAKRVEVMREEI
                     AAVMVDMMQYVVDYGTGQRLRRPEYGGITSQVAGKTGTTQENTDGWFMGMVPQLVCGV
                     WVGADDPVVHFRSTSLGQGANMALPIYGFFMKRVYADKTLGIDPAAKFPLFEGTPTIE
                     LDCGKYIDMQNDDYQDLMEGYN"
     gene            7463..9268
                     /gene="uvrC"
                     /locus_tag="PLB46_02965"
     CDS             7463..9268
                     /gene="uvrC"
                     /locus_tag="PLB46_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRF75505.1"
                     /translation="MDIEAFKNIEINLPNKPGVYRYYDKDNTILYVGKAKNLKKRVAS
                     YFQKTDHSARIKLMVKKINQIEFTIVNSESDAFLLENTLIKQHQPKYNVNLKDGKTYP
                     YIVVKNERFPRIFFTRKKINDGSQYLGPFTSVGKVKSIFEFIKSVFPIRTCNYLLSET
                     NIKNKKFKICLEYHLGNCKGPCEGLMTEEAYMENVQNIVYILKGNLSFVKNAFKQKIQ
                     EHVEQLNFEAAEEVRVKLENIENYQGRSTVVNTNIDNVDVFAFSDAESFCVIGYLKIV
                     QGMITQATTLVLTKKLDETPEELLQIAITELRIQFDTNAREIIVPFKTELSDEQIIQT
                     VPLAGEKKKLLELAYKNALYVREEKSQGKEKRDARNQNLRILETIKADFKLQQLPYHI
                     ECFDNSNIQGTNPVASMVCFKNAKPSKKDYRHFNIKTVVGPDDFASMFEIVTRRYKRL
                     LDEAEPLPQLIVIDGGKGQLGAAVQALKELDLYGKVAICSIAKKLEEIYFPEDPFPLY
                     INKKSESLKVIQQLRDEAHRFAITFHRLKRSKAAIGSELNNIKGIGPNTVKELLKTFK
                     SVKNVKTATPEALEAVVGAAKTQVILNYFNNIPSS"
     gene            complement(9387..10223)
                     /gene="gldN"
                     /locus_tag="PLB46_02970"
     CDS             complement(9387..10223)
                     /gene="gldN"
                     /locus_tag="PLB46_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HRF75506.1"
                     /translation="MKRLFVLSFVMFIVATAMYAQPTTPSAPRQDGIYKRTLLGTREI
                     IPYDDIREADIYWEKRIWRNIDFREKINLAFTWPVDPFVQIVYDAVVSGELKAYSPLY
                     DDFSEGTELPLEQIIIKFNRTDTTWILDEETYEEKPVIIKTEFDYQTVQKIQIKEDWV
                     FDEETSTMVVRIIGLTMIRDRIDPTTGETLGSEPMFWIYYPDLRSIIVRHEVFNEKNS
                     ARYLTFEDIFEMRLFSSYIVKEDNVYDRFIENYTSGIDQVLESERIKQQIFDFEHNLW
                     EF"
     gene            complement(10241..11845)
                     /gene="gldM"
                     /locus_tag="PLB46_02975"
     CDS             complement(10241..11845)
                     /gene="gldM"
                     /locus_tag="PLB46_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HRF75507.1"
                     /translation="MSIPKEPRQLMINLMYLVLTALLALNVSAEILNAFNTVNKGIGD
                     SNELLNSKNELMMGMITKAAAKDGRAETQAILKQAEEAKKLSADFVAYVEDLKNKVKE
                     AAGGPSEENPEKLKTEGEMEKTSNLFLNQGKGVELEKKISETREKFIAILGKESGIDV
                     PLKVSEVPAGEHAKNWEEYNFDRVPAIAVMTILTKFQQDAKSAESTILDYLRRKINAE
                     DIQVDKMAAQVVAPSAYVRKGAEYTADIFIGATSEAIKPKVFIGSFTGAVQKDPEDPN
                     GKSYIMVESETMPLSGAREIEVVGGMGKIKETASGERKFQGVIQIPSPSKEGQFRFYP
                     FEFGYETFEVGKAVVSPTAMNVLYIGVDNPIKISVPGYASDKVSASGCGIVKKSGEEY
                     NAKPTTVGEADITVTVKTAKGTETSAEKFRVRRIPDPYAYILNTKGGPLKIGEFKAAD
                     GLIVKNPDFVFQIPYTVTSFEMIYAPKVGNVVSDVSNSSKFSTIMKDIQKKAKPGDTI
                     VLQNVTVKMPDGRNVPVNTSFKLIGG"
     gene            complement(11893..12717)
                     /gene="gldL"
                     /locus_tag="PLB46_02980"
     CDS             complement(11893..12717)
                     /gene="gldL"
                     /locus_tag="PLB46_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HRF75508.1"
                     /translation="MSAFFESDRFKRIKNLIIGCGASVVLIGALFKIMHWEGADFMLM
                     VRMLTEAVLFLMLGILPPHKDYYWEKIYPDLDIAPEVEEAKSGKKAVHKMEPRESVTR
                     QLDKMLEDSKVEPVMIERLGENLKRLGENIEKLSDVQDAAVNTNEYSTKIKDASMAVA
                     EMRDAYSNAAKAMEQITASSVDTTKYHEQVQMVTKNLGQLNAIYELELQDTNNHLKAM
                     NKFYGSLNSAMDNLQDSVTDTAKYREEIAQLSNNLAALNRVYGNMLSAMSMGANRS"
     gene            complement(12794..>13286)
                     /locus_tag="PLB46_02985"
     CDS             complement(12794..>13286)
                     /locus_tag="PLB46_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF75509.1"
                     /translation="VDDFRLPTEHEWEYAARGGRDQAPYPWGGPYVRNTKGCYLANFK
                     PGRGAYPEDGGYYTVKVYSYAPNDFGVYNMSGNVSEWTSTAFSENSYSFIHDENPDYR
                     YDAEESDPPALKRKVIRGGSWKDISYYIQTGSRHWEFQDTAKCYVGFRCCMTFLGRSM
                     SDF"
BASE COUNT         4369 a         2435 c         2429 g         4053 t
ORIGIN      
        1 gttggtgcct ttcacaaatg atccaaatcc agttgaagca gggcttccgc ggtaaaaaac
       61 agttgtgctt aaattttccc ctgaagataa ttcttcacca aataatattt caacggcatt
      121 ggtaatacga ttaaaatcaa tatttatttc attgagccaa accgagtcaa taattaaatt
      181 atctgctgca tcataaaaaa ttgatgtcgc attttgagta agtgtaaaat gcgtttctac
      241 acttccattt atcatgagtg tatcaggatc gatattccaa ttacatttat gataaaaaat
      301 atcataatta ccggtttctg ctgcatcgaa tgctgacgtt ttatatgcag cctgatggag
      361 ttcttttttt attacttcat tattaaactg gtcaatgttt atgtgctgtg cagatgttac
      421 agctgctaca aacatcaaaa ccgataacca tttaaaacga agccacatta tttcacaatc
      481 ggattttttg gaggagcatt attgccgagc gctttagata atttaaccag ctccagcgtt
      541 tcgaatatga gaaaagaaaa atagatgagg gcaaatggcg ctataaaagc aataccacca
      601 acatcgtttg ttgatttata aaccattaca actattgccg acaaaattaa tttagcaaaa
      661 atgctgacaa aaaatgcacc cataaaattg ctgaattttt gtcgcatagt ccttgctgaa
      721 aaataatata tgacaattgc caaaacaaaa aacaacacaa aacaaatcca aacaaaatcc
      781 attgcacctg aaaacatttc gagtttacct aatccaaaca aagccaatgc aacaactgca
      841 gaaaacggaa taaaacgttt caaaaaatct atcatatatt acttcttatt attcttttta
      901 tttttatttg gcaaaataaa atccttcagt acggcataca tacctgccgc tatgccaaat
      961 aaagacaaaa ttacggtaaa ccagggttta gtgtgaagca cctcatctaa tttcattccg
     1021 ccccaaaccc caagaaaaat tccggcaaac aattggaatg ccattccgga atacttgaaa
     1081 taactgtcaa actgtggctt cttcgtattg cgctgaggca ttagttagtt ttctttcttt
     1141 ggtgcccata ttgcaaatac catcaaaatg agctccgttt tctataacaa tttttgcagt
     1201 ataaatgttt ccttccacta ttgcagttga ttttaaagta agcacttcac ttacttcaag
     1261 gtcgccaatt atttttcctt ccacatcagc acttttgcag tgaacatcac cttcaattac
     1321 accggtttgt cctactgcta ctttggcttt ggtatatagg gttcctcgaa taatgccctc
     1381 aatcctaata tcaccatcgc tggtaatact tccctcaaag gtagtaccat ggttaatcct
     1441 gttggaaata aaagaacctg gtgcagactg ggggtttgat tgtttattgc cgaacatatc
     1501 agtataaaat tttagttgaa gttaataaaa tcttttggat taagcggagt tttttcatgc
     1561 cacagttcaa aatgtaaatg cgggcctgtg ctcatttcgc cggtatttcc tataatggct
     1621 ataacatctc cggctctgat gatattgccc tgccgtttta aaatctggct attatgctta
     1681 taaatactga ttaaaccatg gtcgtggcgg ataataatta cgttgccgct ttccaaattc
     1741 cagtatgaat ctataacaac accgtctaaa caggctttaa ttggggtgtt ttcaggtgca
     1801 actacatcta ttccatagtg accttccctg gtttggaatg aggaggtaac agttcctttt
     1861 ataggaggga aaaaatgcaa agattccaga ttttgctcac cggtggcatc ccttgggtca
     1921 gccaaactca aattaaagag gtcttcacgt gccacttgtt cgcgcatagc tttatctatt
     1981 tcagaagcct cacccacttt gattccgcta taatcctgtt tacttagact atctattgga
     2041 ggtaaagtat caatttcgcc gttcaaacgc ttctgcaagt tggcgaaata tacctcataa
     2101 gacttcagtg ccgtaagcat actgtcggtt attttgttgt tttccgcaat ttgtcgggtg
     2161 gtagaaactt cgctataacc tactaccaaa ttctttaatg gagtgaatac aatggccaga
     2221 taaaccaaaa atccgataac gacaaatagt gtgctggaca aaattatcac gtttaagggg
     2281 cttaaacgca gcgatgcctg aacctcaaaa gtcttatcgt gcataagcac cagcttatat
     2341 ttggtgcgta accgctccag ccattttctt gtttttttgt tacctttcgc cattagtccc
     2401 taaaacacta tattttttac gttatttaaa atatttttgc tgaaaagctg ttatatatcc
     2461 ggcataaaac taactacctt gaaacgaaat tctgtcatat ttacactaac aattatagtt
     2521 cttttttttg tatccgcatg ttccccgggt aaaaatgcag acaaaagctt tatcggacgc
     2581 agtgttgacg accttatagc tcgtgataat ggctacttta atgccaggtt aatcatgaaa
     2641 gaaacggagc acacactttg gaccgatcag gtcgataact atgatgaaac cctaccgata
     2701 tttaaatatg gcacccagga gcaggcaact tctatacagc cttcaatgga tgaggtaatt
     2761 aaaaaaacct catttgtcat ccaaatgcat aaaaaaagta agtgggtaga tgatagtttt
     2821 ttcctgatag ggaaatcaca gttttataag cgaaattatg acgaggccct cacatctttc
     2881 cagtatatca tctccgaata cagcaatttg attgagaaac aatctaaggc aaaacgagta
     2941 gctgaggatg aggatggtga actcactttt tttgaaaaac ttaaacatca gcctgtttct
     3001 tccgaagccg gtatttgggt ggcaagaacc ttagtagaaa aaaaacaata cagtgatgcc
     3061 cataccgtaa ttagtgtttt aaagagccgt gaaaatttcc ctaaatggtt aattggcgaa
     3121 ttatatgcag ttgaagctga tctttacatg aaagaaggcc aagaagcaaa tgctattgaa
     3181 cctttaatta atgcattaaa aaatactaac gacaaagcat taaccaatag atacaatttt
     3241 attttggcgc aattatatgc tcaacaaaaa aatattgata aagcccttgc tacttatcag
     3301 cttgttttag ataacaagcc ggtttatgat atggaatttt acgcccaatt aaatattgcc
     3361 aaattgagtc tggcaaattt tggcgtttct ggtggcgaat caaaagaaga gttaatggct
     3421 ctacttaaag atgaaaaata caaagacttt tacggtttaa tatattatac acttgcagat
     3481 attgcattag ctaataaaga cgaggtaacc ggagttgatt atttaaataa atcgttaaga
     3541 agtacaaacg atagcaaaca aaaaggacaa tcctatttgc gacttgccga tttgtattat
     3601 gccgacttag attataaagc tgcatatgct tattacgaca gtacgataaa tcaaattcaa
     3661 cgtgagcatg aacgttacac tgaagtgcgt ggcttacgcg ataatttaaa attactagtc
     3721 gacgagctta ctattattga aacagaaaag aaattacaat attgggctgg tttgtcggaa
     3781 aaagaattgg aaaaagaatt agctaaatta attcctgaaa aagaagaaac agactcactg
     3841 gctaccgacg tactaaatcc caacaacgta aaaccactgg caggcaaccc aactgaaggt
     3901 gatttttatt tttacaatac aaatttacgt tctcgcggtt tttcagaatt taaaaagaac
     3961 tggggcacaa ggaaactgga agataactgg cgccgaagtg aaaaaggcac atttagcgaa
     4021 gaaggtggta cagagccaat agtcggtgaa gaaaaaacta ttgacctcaa tgcgaaaaat
     4081 gtaaccacag aaaaaattat tgcgagtttg ccaaaaacac ctgatcaaat tgctgtttcc
     4141 aatgcaaaaa tagcaggagc attatatcgt gccggtattg tttacaaaga caatttccgc
     4201 aataaaacaa acgctatcgc aatgtttgag gaaaatgtaa ataattatcc tgccaataca
     4261 tttgaagtgc aatcgcttta ttatttgtat cgattaaccc ttaaccctca acaggataaa
     4321 tataaaaatt tattgttatc taaatatcct gaaacggatt acgcaaaaat tatcagcgat
     4381 ccggattatt ttaaacgtga agaagatcgc aaacacgcaa tagaatccta ttacacagtt
     4441 acctatgatg ccttgcaggc caaaaactat gctgaggtta aatccagggc attagctgct
     4501 gactctttat ttaaacccaa tacattaaag ccaaaattcg atttgttaac tgctttggct
     4561 gaagtggaca gcattgaaac atttaaaaac gcattacaaa aaatagcatt aaaatatcct
     4621 gaaaacgaag taggtataag agcgcaggaa atgttggatt atctgcgtcg tggttctgtg
     4681 atggaagatg caggcaaaac aatccctgta gcggcttata caatgaatgc tgaagaggaa
     4741 cattttttca tgtttgtaat gaattcaacc gggaaaaatg ccacaaccat gaaaagtaat
     4801 attgccacct ttaacggcgc taattttagt accgacaacc taaaaattag ttcactcctt
     4861 ttgggtaaag acaacagttt aattttggta aaatcattta acaatgttga gaaagcaatg
     4921 gtatattatt ttgcaattaa agataaccta ggggttttta aagatttaac taaagaaaca
     4981 tttacacctt ttattatatc caaatcgaat tatgttcaat tttttaaagt aaaagatgta
     5041 gaagcttacg atgtatattt tcaggataac tacctaaagg aataaaacaa acttaatgtc
     5101 aatcaaagaa caatttgctg tttataaaca acagataaaa tggttttggc gcatcttttt
     5161 tattggtgcc ggagttgtta cgttgtttat tatattatta tcttttggtg tttttggaaa
     5221 attgccatcg tttgaagaac tcgaaaaccc aagaagtaat ttatccactc aactaatttc
     5281 ttcggatgga gagttactcg gtcggtttta tcgcgaaaac agaaccaatg caacatataa
     5341 agaattgcca ccacatctta ttgatgcact agtttcaaca gaggataggc gatattatga
     5401 acatagcgga attgatgcca aaagtgtaat gcgtgttatt ttcaaaacac tcatacttca
     5461 acaatcagaa tcaggtggtg gtagtacttt aacccaacaa ttagctaaaa atttatatgg
     5521 tcgtccaact gtaagtaata agtttgagtt ggcggttacc aaattaaaag aatgggttac
     5581 tgcagctaaa ttggaacgtc gctataccaa agaagaaatt attgtgatgt atttaaatac
     5641 cattccatac atttatgatg cgagtggtat taaaacagct tcaaaaactt ttttcaataa
     5701 atcggtaagt gatttaaccg ttgaagaagc agcagtattg gttggcatgg tacaaaatcc
     5761 tgctttttac aaccctgttc gttttcctga gcgtacgtta gaacgcagaa atattgtact
     5821 acatgaaatg catgaagccg ggaaattaag tgataaagat tatgaagcta caaaagccaa
     5881 acccctggtt ttagatttta aacgccagga tcataaatca ggaaacggta cttattttag
     5941 agaatattta cgacaatggc ttgctgattg ggttgaagaa aatgaaaaac ctgacggaac
     6001 aaaatgggat atttataaag acggcttaaa agtatatacc accattgaca gtcgtatgca
     6061 aagttatgca gaagctgctt cttcacaatg gttaacaaaa ttgcagaaac aatttgatgg
     6121 ccattggaaa ggaaataaat atgaaccttg ggcgtatccg tatagtggcc gaacaaatcc
     6181 tgaattgatt gaaaaatcaa taaaacaaac tgagcgctat cgcaaaatga aaaaagcagg
     6241 tgccagtgaa gatgaaatta ctaaggcttt tcatacgcct gatactatga ctgttttttc
     6301 atggtataaa gaaggttatg atttagatac ggtaatgacg ccgtatgact ccattaaata
     6361 tgtaaaaaag atattacatc ccggatttat ggcaatggat cctcacacag gtgagattaa
     6421 agcctgggta ggtggcgttg atatgcgata ttttcaactg gaccacgtaa aacctgctca
     6481 aggccgacag gttggttcaa cgtttaaacc atttgtgtat actgctgcta ttgccgataa
     6541 aggtttttct ccttgttacc aaatacccaa tttgccggta acatttgaaa aggggcaaaa
     6601 atggagttta atcagcgact ggacgccaaa aaactcagat gggtcttatg gtggtatgct
     6661 cactttatcg caaggtttag gtaaatcggt aaatacggta actgcatatt tattgcgtga
     6721 ggtgggtatt caaccagtta ttgatttggc tcacgatatg ggaattgagt ctgaaattcc
     6781 ttttcaacct tccatatgtt tagggacacc ggaagtaact gtgtatgaaa tgaccgccgc
     6841 ctattcggca tatgccaata aaggtgttgc cgttcgtcct gtttttgtaa cgcggattga
     6901 agataaaaat ggcaatatca tagcggaatt ccctgcaaaa cgtgtagaag ttatgcgtga
     6961 agaaattgcg gcagtaatgg ttgacatgat gcaatatgtt gttgattatg gaacaggtca
     7021 aaggttacgc agaccggaat acggcggtat aaccagtcag gtagcaggta aaaccggtac
     7081 cactcaggaa aatactgatg gttggtttat gggtatggtg ccgcaattgg tatgtggggt
     7141 atgggttggc gccgacgatc ctgttgtcca ttttaggtca acttcattag gacaaggggc
     7201 aaacatggca ttacctattt atggtttctt tatgaaacgc gtttacgcag ataaaacatt
     7261 gggtattgat ccggcggcta agtttccgct atttgaaggc acaccaacaa ttgaattgga
     7321 ttgcggcaaa tatatcgaca tgcaaaacga cgactatcag gatttaatgg aaggatacaa
     7381 ttagaacaat ctaaaaaaaa ttccgggaca gccgttatta aacagctgtc cttttttatt
     7441 ttttgtgcta tacatttgta aaatggatat cgaggcattt aaaaatattg aaattaacct
     7501 gcccaataaa cccggtgtat atcgctacta cgataaagac aatactatat tgtatgtagg
     7561 caaagcgaaa aatttaaaaa agcgggttgc ctcttatttc cagaaaaccg accatagtgc
     7621 ccgcattaaa ttaatggtta aaaaaattaa ccagatagaa tttaccattg taaattccga
     7681 atcagatgca tttttattag agaatacgtt gataaaacaa catcaaccaa aatacaatgt
     7741 caatttaaaa gatggcaaaa cttatcccta catagtagtt aaaaatgaac gctttccgag
     7801 aatatttttc accagaaaaa aaataaatga tggttcccaa taccttggtc cttttacttc
     7861 ggtaggtaag gtgaaatcta tttttgaatt tattaaatcg gttttcccaa ttagaacttg
     7921 taattatttg ctttctgaaa ccaacataaa aaataaaaaa tttaaaattt gtttagaata
     7981 tcatttgggg aactgcaaag ggccttgtga agggttgatg acagaagaag cctatatgga
     8041 aaatgtacaa aacattgtat acattttaaa aggtaattta agttttgtca aaaatgcttt
     8101 caaacaaaaa attcaggaac atgtcgaaca attaaatttc gaagctgcag aagaggtgcg
     8161 cgtcaaactt gaaaatattg aaaattatca aggtagaagc acagttgtaa atactaatat
     8221 cgacaacgtt gatgtttttg cattttcaga tgctgaaagt ttttgtgtta tcggatattt
     8281 aaaaattgtt cagggtatga ttacgcaggc aacaaccctg gtacttacca aaaaactgga
     8341 tgaaactcca gaagagttat tacaaatcgc cattactgaa ttgcgcattc aatttgatac
     8401 taatgcaaga gaaattattg ttccgtttaa aacagaacta agtgatgagc aaatcatcca
     8461 aacggtgcca ttggctggag aaaaaaagaa gttgcttgaa ctggcataca aaaatgcatt
     8521 atatgttcgc gaagaaaagt cgcagggaaa agaaaaacgt gatgcacgca atcagaactt
     8581 acggatttta gaaactataa aggcggattt caaattgcag caattacctt atcatattga
     8641 atgtttcgac aactctaaca tacaggggac taatcctgtt gccagtatgg tatgttttaa
     8701 aaatgccaaa ccgagcaaaa aagattaccg gcattttaat attaaaactg ttgttggccc
     8761 cgacgatttt gccagtatgt ttgaaatcgt aacacgcagg tataagcgat tattagatga
     8821 agccgagcca ttgccacaat tgatagtaat tgatggcggc aaggggcaat taggcgcagc
     8881 tgtgcaggca ttaaaggaac tggatttata cggcaaggtg gccatttgtt ccattgctaa
     8941 aaaattggaa gaaatttatt tccctgagga cccttttccg ttgtatatca ataaaaaaag
     9001 tgaaagctta aaagtgatcc agcaattacg tgatgaagcg catcgatttg ccatcacttt
     9061 ccatcggtta aaacgaagta aggcagctat aggctccgaa ttaaataata tcaaaggaat
     9121 tggccccaac accgtgaaag aattgctaaa aacatttaaa tctgtaaaaa acgtaaaaac
     9181 agcaacaccg gaagcattag aagcagttgt tggtgcagcg aaaacacagg taattctaaa
     9241 ctacttcaat aacataccat catcttaatg tttcctatta cgtttaattt ttaacataac
     9301 gttacacatt caagaatggt ataaaaagtg aagaccggca aaaaatgccg gccttcggtt
     9361 caagtatgaa tagaaaagaa agatgattaa aattcccaaa gattgtgttc gaaatcaaat
     9421 atttgttgtt tgatacgttc actttcgagt acttgatcaa taccagaagt atagttttca
     9481 ataaagcggt catacacgtt atcttctttt acgatataac tactgaataa acgcatttca
     9541 aaaatatctt caaatgttaa atatcgggca ctgttttttt cattaaaaac ttcgtggcga
     9601 acaataatac ttcgcaggtc aggataataa atccagaaca ttggttcaga acctaaagtt
     9661 tcaccggttg tagggtcgat acggtcgcgt atcattgtca gcccgatgat acgaacaacc
     9721 atagttgatg tttcttcatc aaatacccag tcttctttta tctgaatttt ttgtaccgtt
     9781 tggtagtcga attcagtttt aataattacc ggtttttcct cgtaggtttc ttcgtccaat
     9841 atccatgtag tatcagtacg attaaattta attatgattt gctccaaagg aagctcagtg
     9901 ccttcgctaa agtcatcata aagagggcta taagctttca attcaccgga aacaactgca
     9961 tcataaacaa tttgaacaaa aggatcaaca ggccaggtaa aggccagatt gattttttcc
    10021 ctgaagtcaa tatttctcca gatacgtttt tcccaataaa tatctgcttc gcgaatatca
    10081 tcgtaaggaa ttatctcacg cgttccgagc aatgttcttt tataaatacc atcttgtcta
    10141 ggtgcgctag gcgtggtagg ttgcgcatac attgctgtag cgacgatgaa cattacaaat
    10201 gataaaacaa ataatctttt catactactt gaatttacaa ttaacctccg attaatttaa
    10261 atgatgtatt taccggaaca tttctaccat caggcatttt aacggttacg ttttgtaaca
    10321 cgatagtatc accaggtttt gcttttttct ggatatcctt catgatagtt gaaaatttgc
    10381 tagagttaga aacgtcagaa acaacgttac ctactttagg tgcataaatc atttcaaagc
    10441 tggtaactgt ataaggaatc tggaatacga aatccggatt ttttacaatt aaaccatcag
    10501 ctgctttaaa ttctccgatt ttaagcggac cacctttagt atttaagatg taggcatatg
    10561 gatccggaat acgacgtact ctgaattttt ctgcggatgt ttcagtgcct ttagcagttt
    10621 tcactgttac agtaatatca gcttcaccaa ctgttgtagg tttagcatta tattcttcac
    10681 cacttttttt aacgatacca caacctgaag cacttacttt atcagaagca taacccggaa
    10741 cagaaatttt gattgggtta tcaactccga tataaagtac gttcatagct gtaggagaaa
    10801 caacggcttt acccacttcg aaggtttcgt aaccgaattc gaaaggatag aagcggaatt
    10861 gaccttcttt acttggagaa gggatttgga ttacgccttg gaatttgcgt tccccagatg
    10921 ctgtttcttt aattttaccc ataccaccaa caacctcgat ttcgcgagcg ccgcttaatg
    10981 gcatagtttc actttctacc atgatataag atttaccgtt tggatcttca gggtcttttt
    11041 gaacagcacc tgtaaacgaa ccgataaata ctttaggttt aatagcttca gaagttgcac
    11101 cgataaaaat gtcagcagta tattcagcac ctttacgcac ataggcagaa ggagctacaa
    11161 cctgtgcagc cattttatca acctgaatat cttcagcatt aattttacgt cttaagtaat
    11221 caaggatagt actttcagca cttttagcat cctgttggaa ttttgttaag atagtcatta
    11281 cagcgatagc cggaacgcga tcaaaattgt attcttccca gtttttagca tgctcacctg
    11341 ctggcacttc agaaactttt agcggaacat cgatacctga ttccttacct aaaatggcaa
    11401 tgaatttttc acgtgtttcg ctaatttttt tctcaagttc tacaccttta ccctgattca
    11461 aaaagagatt actggttttt tccatttccc cttcagtttt taacttctca ggattttctt
    11521 cacttggccc accagcggct tcttttactt tatttttcaa atcttcaaca taagcaacga
    11581 aatcggcgct cagttttttt gcttcctcag cttgttttaa aatagcctgc gtttcagcac
    11641 gaccatcctt tgcagctgct ttagtaatca tacccatcat cagctcattt ttgctgttga
    11701 gtaattcatt actatcacca atacctttat taacggtatt gaaggcattc aaaatttccg
    11761 cagatacgtt caatgccaga agggctgtaa gcaccagata catgaggtta atcatgagct
    11821 ggcgtggttc cttaggtatt gacatttgta tttaaatttt attggttaag taaattttta
    11881 tcaatcaatt aattatgaac ggtttgcacc catgctcatt gcactcaaca tattaccata
    11941 aacacggttt aatgctgcga ggttattaga caattgagca atttcttcgc ggtatttagc
    12001 agtatcagta actgaatcct gaagattatc catagcgctg ttaaggctac catagaattt
    12061 gttcattgcc ttgaggtggt tgttagtgtc ttgcaattct aattcataga ttgcatttaa
    12121 ttgacctaag tttttagtaa ccatttgaac ttgctcatgg tacttagttg tatctacaga
    12181 agatgcagta atttgttcca ttgctttagc agcattagaa taagcatcac gcatttcagc
    12241 tacagccata gatgcatctt taatttttgt agaatactca ttagtattta cagcagcatc
    12301 ttgcacatca gaaagttttt caatgttttc gcctaaacgt tttaagtttt cacctaaacg
    12361 ttcaatcata acaggttcaa ctttgctgtc ttccaacatt ttgtctaact gacgggttac
    12421 actttcgcgt ggttccattt tatgaaccgc ttttttacct gatttagctt cttcaacttc
    12481 aggtgcgata tccagatccg gataaatttt ttcccagtaa taatctttgt gtggaggaag
    12541 gataccaagc attaagaaga gaactgcttc ggtaagcata cgaaccatta acatgaaatc
    12601 ggcgccttcc cagtgcatga ttttgaagag agctccaatt agtacaacag aagcaccgca
    12661 accaatgatc aggtttttaa ttcgtttaaa acgatcggat tcgaaaaatg ctgacatttt
    12721 gttttaaatt ttaagttttt aaatgattgt taacaaatag ctgtgttcaa taagaattgg
    12781 ttaggaattt attttagaaa tcgctcattg aacgaccgag gaaggtcatg caacaacgga
    12841 atccaacata acatttagca gtatcctgaa actcccaatg acgtgaacct gtctggatat
    12901 agtaactaat atctttccat gaaccgcctc tgattacttt acgttttaat gccggtggat
    12961 cactttcctc agcatcataa cgataatcag ggttttcatc atgtataaat gaataactat
    13021 tttcagaaaa agctgttgaa gtccactcag aaacgttacc ggacatatta tatacaccga
    13081 aatcgtttgg tgcataagaa taaaccttaa cggtatagta accaccatct tcagggtagg
    13141 caccacgacc aggtttaaag ttagcgaggt aacatccttt ggtatttctt acataaggac
    13201 caccccaagg ataaggagcc tggtctcttc cgccacgagc ggcatattcc cattcgtgtt
    13261 ctgtaggtaa acggaaatca tctacc
//
LOCUS       DAORKW010000038        12678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_75_length_12678_cov_4.889487, whole genome shotgun sequence.
ACCESSION   DAORKW010000038 DAORKW010000000
VERSION     DAORKW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12678
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75_length_12678_cov_4.889487"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PLB46_02990"
     CDS             complement(<1..772)
                     /locus_tag="PLB46_02990"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRF75510.1"
                     /translation="MRWIISLCLLSVMVNISCSEELPGQQPFPLYDHIVVVFMENHDY
                     ATMVDTSAAAYIHQLMSDPNTANFTRSYALGHPSQPNYIMFFSGDNQGVTTNDKPISS
                     FTTPNLAAQLVDEGFTFVTYSEGLPYPGYDGEIFERYVRKHNPVTNWMGPKRNQVDSA
                     LNQPFTAFPTDYNELPTVSFVIPNLDNDMHDGSIAQGDAWLKTFFEPYIEWAKTHNSL
                     FILTFDEGSYAEDGDHIITTFTGQHIIGGDYNEIINHYN"
     gene            1009..1093
                     /locus_tag="PLB46_02995"
     tRNA            1009..1093
                     /locus_tag="PLB46_02995"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1043..1045,aa:Leu,seq:tag)
     gene            1194..2570
                     /locus_tag="PLB46_03000"
     CDS             1194..2570
                     /locus_tag="PLB46_03000"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRF75511.1"
                     /translation="MNISIDNLGLQHNLVRFTFTNEDYRGRFDKALKDLSKKAQVPGF
                     RPGHVPMGMVKKMYGDSVILDELYKMVNEQMNAYLKENNLELLGDALPVENDLGVDYN
                     ETKTYEFVYEIGVQPTIDLSINLNKNKSLTRFRIPAKQEEIDQEFERIVRKYGERKDV
                     DSVEENDVVYAHVKELNEDGSATDGGVNVETYFNLQMLNDEHQNLFLGGKPGDVKNID
                     DLFIVFKGDKVKVAKNILHLTEATEETVVNISPKFEIKVDRIARLFPAEINDAFFTEI
                     SKEFGPIATETELREKIAAAIEQYHDSLTEVSLENELFKYLIDTTEVPLPDVFLRKWY
                     SQTNQKDSTAENFEAEFADFTNKLKQSLIYREVQKSHELNVSNDEIIQEALFAVRSSY
                     GQMGEDFVQYILQSQLKDKAFVENMHDRVAQKKFFNVIKEYITIEEQPTTLEAFQQLN
                     KTEEVYAE"
     gene            2560..3249
                     /gene="clpP"
                     /locus_tag="PLB46_03005"
     CDS             2560..3249
                     /gene="clpP"
                     /locus_tag="PLB46_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRF75512.1"
                     /translation="MQNNEFRKYAIQHMGMSSNSLDSYMKTQSMHVTNFTPYIIEERQ
                     LNAQALDVFSRLMMDRIIFMGTAIDDYVANVIQAQLLFLSSVDAKRDIQIYINSPGGV
                     VYSGLAIYDTMHFITPDVATICTGIAASMAAVLLCAGAPGKRAGLSHSRVMIHQPLGG
                     AQGQASDMEITLKEILKLKTELYNIISKHSGQPFEKVEKDSDRDYWMRSAEAKEYGMI
                     DEVLVKDPKKG"
     gene            3269..4504
                     /gene="clpX"
                     /locus_tag="PLB46_03010"
     CDS             3269..4504
                     /gene="clpX"
                     /locus_tag="PLB46_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRF75513.1"
                     /translation="MKKKETAHCSFCLRPKEDVKMLIAGQEGHICETCVSQAQTLVEE
                     ELHAPVKRYKFALANSMKPRAIKEFLDTYVIGQVEAKRSVSVAVYSHYKRLNQKTNED
                     VEIEKSNIMVVGRTGTGKTLLARSIAKFLNVPFAIVDATVFTEAGYVGEDVESILSRL
                     LQVCNYDVAAAEKGIVYIDEIDKIARKGDNPSITRDVSGEGVQQALLKMLEGTEVMVP
                     PQGGRKHPEQKMVKVNTQNILFICGGAFDGIEKIIARRTDTQLIGFKRQNDAVTADRE
                     NLLQYIAPQDLKAFGLIPELIGRLPVLTHLDPLDADVLRAILVQPKNAIIKQYKQLFE
                     IEDINLIIDDAVLDFIVEKAIDFKLGARGLRSICEALLTDAMFELPSNSDIKDFHLTL
                     EYAVNKFNQSKLSLLKIAS"
     gene            complement(4823..5128)
                     /locus_tag="PLB46_03015"
     CDS             complement(4823..5128)
                     /locus_tag="PLB46_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRF75514.1"
                     /translation="MKKKNNNLTSLEDFKVKNYGKRGTKKREELEAGYENFKIGVLLL
                     EARLEKGLTQEQLAAKAGTTKSYISKIENNIKEVRISTLQKIVETGLGGHLELSIKL"
     gene            complement(5112..5456)
                     /locus_tag="PLB46_03020"
     CDS             complement(5112..5456)
                     /locus_tag="PLB46_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRF75515.1"
                     /translation="MEAGKFRTIVFYKDYFQTFFDKLSTKVKAKIIWTLDLIEETHRV
                     PETYLKHIEGTDGIFEIRVQFGSDIYRIFCFFDKGKLVILANGFQKKTQKTPKNEIEL
                     AKKIKAEYEKEK"
     gene            6196..7734
                     /locus_tag="PLB46_03025"
     CDS             6196..7734
                     /locus_tag="PLB46_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75516.1"
                     /translation="MKNLIEISKVVTKKRISKIEIFDKSLLNRKESKFNEFYEGLVND
                     KFNTDEEAAAVLYNTSPLDDKYRQLKSRFSKRLLNTLFFLDSNDPSFSDYHRANYSCN
                     KNWAQIRILLNSGARNAAIKIAAKTLGVAVIYRFSDMILNCSRILMNNASLNGDEKEY
                     EEYAALTNKALRDVEYEIKAEEMYQRLTINFALSAASTGELASTADKYLDAVNKLCEQ
                     SDSYQLHYMKYQIRIMVAQIKGQYLQTIKVCEEAEKYLNDNPNFNQKVKLANLALIKI
                     SSYLQLRDYENGRMNAEKCLDLVAEGSNNWFIFLEYYFLLAMHTENYINAAGIFIKVV
                     NHPRFVYTSEERKEKWKIFESYINYIFETENLDRDLLASDPTKRKFRLLKFLNEVPNF
                     TKDKKGYNVSILVIQIIYMLEKGELTGIINRSEALNVYCSRYLRKDDSYRSSCFLKML
                     LTMVKEDFVYDRTKQMAQKYLRKMQEGDPNGNNMVNEWEIIPYEILWEKVLNRLKHKP
                     ATRI"
     gene            complement(7745..8953)
                     /locus_tag="PLB46_03030"
     CDS             complement(7745..8953)
                     /locus_tag="PLB46_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRF75517.1"
                     /translation="MKTSRRDFIRYSSLASASMFLPQFLKASGNLIGDAARPKVLVVI
                     QFSGGNDGLNCIIPYGNDLYQKARPKIGYKKEEVIKLGDDIGMNTSLQGLADLYFNGD
                     VVLINSVGYPNPNRSHFRSMDIWQSGSDENVYLPTGWLGRVMDSTCDNKDCAKPHFAV
                     ELDDSLSLALKGNYMSGFAMRDPKQLSQVNQNPFIDSIARQQLNFEDDHHNVAYLHKI
                     LADTAESTDYLTEVSKKYTNNAIYPVNEFSNQLKTIAQLIIAESNTSIYYVSLSGFDT
                     HAGQKGQQERQLKVYADGLKAFCNDLKSKGKFKDTMIMTFSEFGRRVDENASLGTDHG
                     TANNVYLISGGLKQGGMINDMADLANLQDGDLIHQVDFRSVYSTLLNKWLNVDAQKIL
                     NGSYPMLDCL"
     gene            complement(8956..10350)
                     /locus_tag="PLB46_03035"
     CDS             complement(8956..10350)
                     /locus_tag="PLB46_03035"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HRF75518.1"
                     /translation="MVSKQQQVQHLYLRAGFGETPAVIKKKQQQSIHKLVDELFDDAK
                     QYKQITYLENPADSKEVGNFKVLLLILKSRDQMQELNIEWLKMLHTTNSVLREKMTLF
                     WHNHFATSTQLAYLMQVQNNTLRKNALGKFDKLLHAISTDPAMIIYLNNQQNKKDAPN
                     ENFAREVMELFTLGEGNLYTEKDIKEAARAFTGWTVNKRGKFEFVAKEHDDGEKEFMG
                     MKGNFNGQDIINILLSKKQTARYVTYKIYKEFVNDDVDETIVTQLTDQFFESGYDIGK
                     LMKTIFTADWFYDEKNVGTHIASPVELLTRYKRLLNIGFKDDKAQLVVQKVLGQVLFF
                     PPNVAGWPGGKNWVDSSTLTLRMNIPTRIVQGGGFDIRPKPEFEDAPEDETALKARKR
                     SEIYSDWASIVTAFKMVPKESLVDAVIEAFIQSPKEGIDKSLIEKYVDHSTDDKRIIS
                     TVGIVMSLPEFQLI"
     assembly_gap    10673..10772
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11084..12487)
                     /locus_tag="PLB46_03040"
     CDS             complement(11084..12487)
                     /locus_tag="PLB46_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75519.1"
                     /translation="MKKLLILLAFFGTVAKAQNLGFNGSGFFENYNAEVEQYLVDMNQ
                     PFTIRVPGGAISKFHDPANVKKGWGMTEANVKTWFDKTGFDEDGNGYEKWLRKTEEQP
                     NHSYLDDLIALQKKFPNMQVLYVLNVLNSTPEKNMQAIRYLMKNNVKVVGVEAGNEIY
                     GKYASFDEYVNDFEPIFTMLTKEYPSIKKGLVAGANLGRKELVRWNESMAQYKGDYDA
                     VIFHYYYTKNDLDEAYNMIPLRIKYDENVTNKDLEKAYDKAAEVLLDKSMIERGVAYA
                     RDLFGDKAIWITEWNTKPSEMLCNTILNGAWQFREMVEQSEQVEYLLMHNGVSPDKYG
                     MISKGTYADSENATMIRRTGYWAYILASEAQGAQKLVKNEAPKIQVDDDGEICIYFVN
                     MDEAFNYTLDLGKLNKTSITINYVSGKHLYSGSGMTGYMGKGSKPSYEVNGIKREKFS
                     GTIPKNAFGYILIKTGK"
BASE COUNT         3836 a         2432 c         2380 g         3930 t
ORIGIN      
        1 cattataatg gttaatgatt tcattataat cgccaccaat aatatgttga ccggtgaagg
       61 tggtaataat atggtcgccg tcttccgcgt aactgccttc gtcgaaagtg agtataaaca
      121 gactgttatg tgttttagcc cattcgatat agggctcaaa aaaggttttc agccaggcat
      181 caccttgggc tattgaaccg tcgtgcatgt cgttgtccag attgggtatc acaaaactca
      241 ccgtaggcaa ttcattataa tcagtcggaa atgccgtaaa gggttgattt agtgcggagt
      301 ccacctgatt gcgtttgggt cccatccaat tggtaacagg gttgtgtttg cggacatatc
      361 gttcaaaaat ttccccatcg taaccgggat atggtaagcc ttcgctgtaa gtgacaaacg
      421 tgaaaccctc gtcaaccaat tgagctgcca gattaggtgt agtaaaactg cttattggtt
      481 tgtcgtttgt ggtaaccccc tgattatcgc ctgaaaaaaa catgatatag ttcggctgac
      541 tagggtgacc aagtgcataa ctgcgggtaa aattggcggt attggggtcg ctcatgagct
      601 gatggatata agctgctgcg gaagtatcta ccatagtagc ataatcgtgg ttttccataa
      661 acactactac aatatggtca tacagcggaa atggttgctg cccgggcagt tcctcgctgc
      721 aggaaatatt taccattaca cttaataaac aaaggctgat aatccatctc acaagggtaa
      781 agatatagtt taatacacta tccctgctgc acttatccgt caaaagcttt tccctaatta
      841 cgggtatcaa ggcctacaag gtaaactgca ttatacttta cctacaactc tccattttcc
      901 cacttatttt tcttaccttt gcatctcttt ttaaaaagga cccggttggc gggcaaaaag
      961 cgtgttcttt atttgatttt acatcaatat tgacatccaa caacaaaagc gggtgtggtg
     1021 aaattggtag acataccaga cttaggatct ggcgccgcaa ggcatggggg ttcgagtccc
     1081 tccacccgca ccataacctc aacagaaagt ctgaagtcct caggctttct gttttttata
     1141 ccaaagggaa cgaaaaatca ttctaataac tttacaataa taatttccaa acagtgaata
     1201 tctcaatcga caacttaggc ttacagcaca atttggtgcg ttttacattt actaacgaag
     1261 attaccgtgg tcgtttcgac aaagcattaa aagacctttc aaaaaaagca caggttccgg
     1321 gtttccgtcc gggtcacgtg ccaatgggta tggtgaaaaa aatgtatggc gattcggtta
     1381 ttctcgatga gctttacaaa atggtaaatg agcaaatgaa tgcttattta aaagaaaaca
     1441 atctcgaatt attaggtgat gcactgccgg ttgaaaacga ccttggcgta gattacaacg
     1501 agacaaaaac ttatgaattt gtttatgaaa ttggtgttca gcctaccatt gacctgagca
     1561 ttaatctcaa taaaaataaa tcgttaaccc gtttccgcat tccggcaaaa caggaagaaa
     1621 ttgaccagga atttgaacgt attgtacgca agtatggtga gcgcaaagat gtggacagcg
     1681 ttgaagaaaa cgatgtggtg tatgcgcatg taaaagaatt aaatgaagat ggttcagcta
     1741 cagatggtgg tgtgaatgtt gaaacttatt tcaacctgca gatgctgaat gatgagcatc
     1801 agaatttatt tttaggtggt aaacccggtg atgttaaaaa tattgatgac ctgtttatcg
     1861 tcttcaaagg agataaagta aaagtggcga aaaatattct ccatttaact gaggctaccg
     1921 aagaaactgt ggtaaacatc agccctaagt tcgaaattaa ggttgaccgt attgcccgtt
     1981 tgttcccggc agaaatcaac gacgcgttct ttacagaaat cagcaaagag tttggcccta
     2041 ttgccactga gactgaatta cgtgaaaaaa ttgctgcggc aatagagcaa tatcacgaca
     2101 gtttaacgga ggtttcgctt gagaatgaat tatttaaata tttaatcgat acaaccgaag
     2161 tgccccttcc ggatgtcttc ttaaggaaat ggtatagtca gaccaaccaa aaagacagca
     2221 cagctgaaaa ctttgaagca gaatttgctg attttaccaa taaattgaaa caatcgttaa
     2281 tttaccgtga ggtgcaaaaa tcgcatgaat taaatgtgag caacgacgaa attattcagg
     2341 aagcgttgtt tgcggtgcgc agcagttacg gacaaatggg tgaagatttt gtgcagtaca
     2401 ttttacaaag tcagttaaaa gacaaggctt ttgtagaaaa catgcacgat cgcgtagcac
     2461 aaaagaagtt ttttaacgtc attaaagaat acatcactat tgaagaacag ccgactactc
     2521 tggaagcttt tcaacaacta aacaaaacag aagaagtgta tgcagaataa cgaatttcgc
     2581 aagtatgcga tccagcacat gggcatgagc agtaactcgc tcgatagtta catgaaaaca
     2641 caaagtatgc atgtaaccaa ttttacacca tacattattg aagaacgcca gttgaatgcg
     2701 caggcactcg acgtgttttc aagattaatg atggaccgta tcatttttat gggcactgcc
     2761 atcgacgatt atgttgccaa tgtaatccag gcgcagctat tatttttatc gagtgtagat
     2821 gcaaaacgtg atatccagat ttatattaat agtccgggtg gcgttgtgta ttccggtttg
     2881 gctatatacg ataccatgca ttttattacc ccagatgtag caacaatttg caccggtatt
     2941 gccgcttcaa tggcagccgt attattatgt gcaggtgctc cgggaaaacg tgccggttta
     3001 tctcattcac gcgtaatgat tcaccagccg ctaggtggtg ctcaaggtca ggcaagtgat
     3061 atggaaatca ccttaaagga aatattaaaa ctcaaaactg agttatataa tattatcagc
     3121 aagcacagcg gtcagccttt cgaaaaagta gaaaaagaca gcgatcgcga ttattggatg
     3181 cgttcggctg aagctaaaga gtatggtatg attgacgagg tgttggtaaa agacccgaaa
     3241 aaagggtaaa tttgattgaa caagttacat gaagaaaaaa gaaaccgccc attgttcatt
     3301 ctgtctgcgt ccgaaagagg atgtgaagat gctgatagca ggtcaggaag gacatatttg
     3361 tgaaacctgt gtaagccagg ctcaaacctt ggtggaagag gaattacacg cccctgtaaa
     3421 gcgttacaaa ttcgcattgg ccaattccat gaagccacgt gctatcaaag aatttcttga
     3481 tacttacgtg attggtcagg tagaagccaa aaggagtgta tccgttgcgg tttacagcca
     3541 ttataaacgc cttaatcaaa aaacgaatga ggatgtagag attgaaaagt cgaatattat
     3601 ggtggttggt agaaccggaa caggaaaaac cttacttgcg cgctctatcg ccaagttttt
     3661 gaatgtgccg tttgcgattg ttgatgctac tgtgtttact gaggccggtt atgtgggcga
     3721 ggacgtggaa agtatattat cgcgtttgct ccaggtttgt aactacgacg ttgctgctgc
     3781 cgaaaaaggc attgtatata tcgacgaaat agataaaatt gcgcgcaaag gcgataatcc
     3841 ttccattacc cgtgatgtat caggtgaagg tgtgcaacag gcattgttaa aaatgcttga
     3901 aggcaccgaa gtgatggttc caccgcaagg gggacgtaaa catccggaac aaaaaatggt
     3961 gaaggtgaat acgcaaaaca ttctgtttat ctgcggcggt gcattcgatg gtatcgaaaa
     4021 aattattgcc cgacgcacag atactcagct cataggcttc aaacgccaaa acgatgcggt
     4081 aactgcggac agagaaaatt tattgcaata cattgcccct caagacttaa aggcattcgg
     4141 tttaattccc gaactcattg gtcgtttacc ggtgctcaca catcttgatc cattggatgc
     4201 tgatgtattg agagcgattc tcgtgcaacc taaaaatgcc atcatcaaac aatacaaaca
     4261 actgtttgaa attgaggata tcaatttaat tattgatgat gccgtattgg actttattgt
     4321 agaaaaagct attgatttca aactcggtgc tcgcggctta cgctctattt gtgaagcctt
     4381 acttaccgat gcaatgtttg aactgccaag taattcagat atcaaagatt tccatcttac
     4441 cctggaatat gcagtaaaca aattcaatca atcgaaattg agtttgttga agatagccag
     4501 ttgatattaa ggaaacttgt ttccgtttgg gttatcagat tgtacagcaa ttattcagca
     4561 tcccattaaa cgattcatta aaagatgttt agcaggaaat gttttgtaat ttaactcagt
     4621 gtcattaagg atacttgtat ccgtgatagt aaggatactc gcatccgctg ggatgaagat
     4681 ttatcggcag taaggtttcg acattccaat aaactcaagc gtaaaaaaat tatcaggttg
     4741 ctctgaattc catacgctga aatacattct actcgagatt aaactaatta aagtggtcag
     4801 gtttgataat aatcagtatc cattataatt tgatggataa ctcaagatgt ccaccaagtc
     4861 cggtctccac aattttttgc agggtcgaaa tgcgaacttc ttttatatta ttttcaattt
     4921 ttgaaatata tgattttgtt gtgccggctt tagcagcaag ttgctcttgt gttaaacctt
     4981 tttccagcct tgcctcaagc agtaaaactc caattttgaa attctcgtat ccggcttcta
     5041 attcttcgcg ttttttggtc ccacgttttc cataattttt cactttaaag tcttcgagcg
     5101 aagtcaaatt gttatttttc tttttcatat tctgctttta ttttttttgc taattcaatt
     5161 tcgtttttag gcgttttttg tgttttcttt tgaaacccgt ttgctaaaat gactagtttt
     5221 cccttgtcga aaaaacagaa gattcgatag atgtcacttc caaactgaac tctaatttcg
     5281 aatattccat ctgtcccttc aatatgcttg agataagttt caggcacccg atgagtttcc
     5341 tctatcaagt ctaaagtcca aatgattttg gcttttactt ttgttgataa tttgtcaaaa
     5401 aaagtctgaa aataatcctt gtaaaaaacg atcgtcctga atttccctgc ctccacaaac
     5461 caaagataca aaagtttccc ttaagtgaaa cttcaaatgc tattatttta tcgggaatgg
     5521 aaaattttgg gtggtttaac atcgcaaatg ggggtaaatg cagcaaaagt tgattggtcc
     5581 gacggcgtaa ctacatgatt aaccgagtgg ctcaatattg gttggttttt gggggatgga
     5641 cctctactta ccacaaacag aaaggaggcc taagcctcct tctggtcgtg aacctaattt
     5701 atgaaacaaa ccaactgtct aaaagttata attcgtcatt gtctataata actatagctc
     5761 caactggaaa ttttgtaggt aattctcttt tcatagttat tgttgttttg gctgttaact
     5821 gaatgactaa cgaagtcaaa agtaagtggc ttaaatgatg cttccattac agggacgagt
     5881 tatttgtgac tatatatttt aaactttttt aacctataac tgcctctaat ttaaccattt
     5941 acgccattaa ttaaataatt tactatcatt ttgtgacatt ttaaaataat tatagacatg
     6001 tgaattaata tctctttagt ctcaaaaagt gtgcataatg cgtagaaaaa agaattccaa
     6061 ttttgttagg aattaactat ttttgttatg tttgtgacac aaattacgcc tcttaaaaac
     6121 ggttccccgg taaaaaaccc ttaatttcag gctttaatca ttagtattga cacattaacc
     6181 aagcgcacac atagtatgaa aaacctaatt gaaatttcaa aagttgtgac taaaaagcgg
     6241 atctctaaaa tcgagatttt cgataagagt ctgctcaatc gtaaggagag taagttcaat
     6301 gagttctacg aggggttggt gaacgataag tttaatacag acgaagaagc tgctgctgtg
     6361 ctgtataaca cttctccgct tgatgataaa tatcgtcagt taaaatcacg tttttcgaaa
     6421 aggttgttga ataccttgtt ttttctggat agtaatgacc cttcattttc ggattatcat
     6481 cgcgcaaatt attcctgcaa taaaaactgg gctcagataa gaatattgtt aaacagtggt
     6541 gcgcgtaatg ctgctattaa aattgccgct aaaacattag gcgttgctgt tatttaccgt
     6601 ttttcggata tgatattaaa ttgttcacgc atattaatga acaatgccag cttaaacggt
     6661 gatgaaaaag aatatgaaga atatgctgcg ttaactaaca aagcattacg ggatgttgag
     6721 tatgaaatta aggctgaaga aatgtatcag cgccttacca tcaattttgc attaagcgcg
     6781 gcgtcaaccg gagaactggc atctactgca gataagtatc tggatgccgt aaacaaactg
     6841 tgtgagcaaa gcgacagcta tcagttacat tatatgaaat atcaaattcg tataatggtt
     6901 gcgcaaatta aaggtcagta cctgcaaacc attaaagttt gtgaggaagc tgaaaaatat
     6961 ttaaacgaca atcctaattt taatcagaaa gttaaacttg ccaacctggc attaattaaa
     7021 atcagttcgt acctgcaact ccgcgattat gaaaatggtc gcatgaatgc agaaaaatgt
     7081 ctcgacctgg ttgccgaagg aagtaacaac tggtttatat tcctcgaata ttacttttta
     7141 cttgccatgc ataccgaaaa ttatatcaat gcagcaggta tatttattaa agtggtaaat
     7201 cacccgcgtt tcgtttatac cagcgaggag cgtaaagaga aatggaaaat tttcgaatcg
     7261 tacatcaact atattttcga aaccgaaaat ctggaccgcg atttattagc cagcgaccct
     7321 acgaaacgaa aattccgttt gttgaaattc ctgaatgagg taccgaattt taccaaggat
     7381 aaaaaaggtt acaatgtatc tatcctggta attcaaataa tttacatgct cgaaaaaggt
     7441 gagctcaccg gtatcatcaa tcgttcagaa gcattgaatg tttattgcag tcgctactta
     7501 cgcaaagacg actcctatcg cagcagttgt tttctcaaaa tgttgctcac tatggtgaaa
     7561 gaagattttg tgtacgaccg caccaaacaa atggcgcaaa agtatttacg caaaatgcag
     7621 gaaggtgatc caaacggcaa caacatggtg aatgaatggg aaattattcc ttacgaaata
     7681 ttatgggaaa aggtacttaa tcgcctcaaa cataaaccgg ccacccgcat ttaatttttt
     7741 cggattacaa acagtccaac atcggatagc tgccatttaa tattttttgg gcatctacat
     7801 ttaaccattt gtttaacaat gtggaatata cactcctgaa atctacttga tgaattaaat
     7861 ctccatcttg cagattagct aaatcagcca tatcgtttat cataccacct tgttttaatc
     7921 cgccgctgat taaatacaca ttattcgcag ttccgtggtc ggtacctaaa cttgcatttt
     7981 catctactct tctgccgaat tccgaaaatg tcataatcat ggtatcctta aatttacctt
     8041 tggattttaa atcattacaa aatgctttta atccgtcggc atacactttt aactgacgct
     8101 cctgttgtcc tttttgtccc gcatgtgtat caaaaccact cagcgataca taataaatgg
     8161 aggtatttga ttctgcaata ataagttgag caattgtttt taattgattg ctgaattcat
     8221 ttaccggata aattgcatta ttcgtatatt ttttggatac ctcggttaaa taatcggtgc
     8281 tctctgcagt atcagccaaa attttatgca agtaagcaac attatgatgg tcgtcttcga
     8341 aatttaattg ttgtctcgca atggagtcaa taaatggatt ttggtttacc tgacttaatt
     8401 gttttgggtc acgcatagca aatccgctca tgtaattacc ttttaaggca aggcttaacg
     8461 aatcgtcgag ttccactgca aaatgtggtt tagcgcaatc tttattatcg caggtgctat
     8521 ccattacgcg gcctaaccat cctgttggca gataaacatt ttcatcgctg ccgctttgcc
     8581 atatatccat acttctgaaa tggctgcggt taggattcgg atatcctacg ctattaatca
     8641 gcaccacatc tccattaaaa tataaatctg ccaatccttg caagctggta ttcatgccaa
     8701 tatcatcccc aagttttatt acttcttctt ttttgtaacc gatttttggt ctggcttttt
     8761 gatataaatc attgccatac ggaataatac aattgagtcc gtcgttacca cctgaaaatt
     8821 gaattaccac caacactttt ggacgtgcgg catcgccaat taaatttccg gatgctttta
     8881 aaaattgcgg taaaaacata cttgcagatg caagacttga atagcggata aaatctcttc
     8941 ttgaagtttt catactcaga ttaattgaaa ttcaggtaat gacattacta ttcccacagt
     9001 gctgatgata cgtttatcat cagttgaatg gtccacatat ttttcgataa gtgacttatc
     9061 aatgccttct ttaggtgatt gaataaatgc ttcaattaca gcatcaacaa gtgattcttt
     9121 tggcaccatt ttaaatgccg taacaattga tgcccaatca gaatatattt ccgaccgttt
     9181 cctggccttc aatgctgttt catcttccgg tgcatcttca aattccggtt ttggtcgtat
     9241 atcaaatcct ccaccctgaa caatacgtgt cgggatattc atacggagtg tgagtgtaga
     9301 actatccacc caattttttc cgccaggcca accggccaca ttcggaggaa aaaataatac
     9361 ttgtcccaac actttctgca ctacgagttg tgctttatca tctttaaatc cgatattgag
     9421 taatctttta tatcgcgtta gcaattccac cggcgatgcg atatgcgtgc cgacattttt
     9481 ttcatcataa aaccagtccg cggtaaatat tgttttcatc aatttcccga tatcatatcc
     9541 cgattcaaaa aactggtcgg taagttgagt tacaattgtt tcatctacat catcattcac
     9601 aaattcctta taaattttat aggtgacata cctggcagtt tgttttttac ttaataatat
     9661 attgatgata tcctgaccat taaaattgcc tttcatcccc ataaattctt tttcaccatc
     9721 atcgtgttct tttgccacga attcaaattt tcctctttta tttacggtcc acccggtgaa
     9781 tgctcttgca gcttctttga tgtctttttc tgtatataaa tttccttctc ccaaagtaaa
     9841 taattccatt acttcacggg caaaattttc gttgggagcg tcttttttat tttgctggtt
     9901 atttaaataa ataatcatag cagggtcggt agaaatggcg tgtaataatt tatcgaattt
     9961 ccccaatgca tttttgcgca gggtattatt ttgcacttgc attaaatagg cgagttgtgt
    10021 tgaggtggca aaatgattat gccaaaacaa ggtcattttt tcgcgcaata ctgaattggt
    10081 ggtatgtaac attttcagcc attctatatt gagctcctgc atttggtcgc gggattttaa
    10141 aatgagcagg agtactttaa aattgccgac ttctttgctg tccgcaggat tctcgaggta
    10201 agttatttgt ttgtattgtt tggcgtcatc aaataattca tcgactaatt tgtgaatgga
    10261 ttgctgttgt ttctttttga tgactgccgg ggtttcccca aagccggccc taaggtagag
    10321 gtgctgaact tgctgctgct tacttaccat ggttgtgatt ttgatgcggt aaaacaattg
    10381 ttgtgccagt aatgaattaa gtatatgaca ctgttgggtt tatagggttt aatgtaaaac
    10441 gattagaggt gttattttgt tacagatacc gtaataaaat cttccctgca tctccgctcc
    10501 gcgcctccgc gtgcaacaaa tccataacat ttacgaaatg tttctcgcaa aggcgccaag
    10561 gtgagggtat tagcactata tgctttgttt gaatataaaa ccaattcaag cgcgcaaagg
    10621 tgtatttgga ttatcaccta aaaaaaaata tcatatagtt tacgaattgt ttnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctcgctaa ggcgcaaagt aaagatttcc
    10801 gtaatatgta ccatgtctaa ttaaaaaacc aattcaagca cgcaaaggtg tatttggatt
    10861 atcacctaaa aaaatatcat atagtttacg aattgtttct cgctaaggcg caaagtaaag
    10921 atttccgtaa tatgtaccat gtctaattaa aaaccaattc aggcacgcaa aggtgaaatt
    10981 ggtttaccag ctttgcgcgc ttaaaaacaa accataccgc ccatcatata ttcaaatcac
    11041 cttagcgcct tggcgagaaa caacaacaca ctttccaatc agctcattta ccggttttaa
    11101 ttaatatgta cccaaacgca tttttaggta tagtccccga aaatttttcg cgtttaatac
    11161 catttacttc gtaactaggt ttagaacctt tacccatata accagtcata ccggaaccac
    11221 tatataaatg tttaccactt acataattta tggtaataga agttttattt aatttaccca
    11281 ggtctaatgt ataattaaat gcctcatcca tattgacaaa gtagatacaa atttcaccat
    11341 catcatccac ttgtatttta ggtgcttcat ttttaaccag cttttgtgca ccctgtgctt
    11401 cggaagctaa aatatacgcc caataacctg tgcggcgtat cattgtggca ttttcactat
    11461 ccgcataagt acctttacta atcattccat atttatcagg gctcacacca ttgtgcatga
    11521 gtaagtattc cacctgttca gattgctcca ccatttcacg aaattgccat gcaccattta
    11581 aaatggtatt acacaacatc tctgatggtt tagtattcca ttcggtaatc catattgcct
    11641 tatcgccaaa caaatcgcgt gcataggcaa caccacgttc aatcatactt ttatcgagta
    11701 aaacttcagc tgctttatcg tatgcttttt ctaagtcttt gttggttaca ttttcatcgt
    11761 atttaatgcg gagcggaatc atattatacg cttcgtccaa atcattttta gtatagtaat
    11821 agtgaaaaat tacagcatcg taatcacctt tatattgcgc catactttca ttccaacgca
    11881 ctaattcttt tctacccaaa ttggctcctg caactaaacc ttttttaata gatggatatt
    11941 ctttagtaag catagtaaaa ataggttcga aatcatttac atattcgtcg aaggaagcat
    12001 atttaccata aatttcgttc ccggcttcta cacctaccac cttcacatta tttttcatga
    12061 ggtaacgaat ggcctgcata tttttttcag gtgtgctgtt taatacattc aacacataaa
    12121 gcacctgcat gttaggaaac tttttttgca gcgcaattaa atcatccaaa taactgtgat
    12181 taggttgttc ttccgttttg cgcaaccatt tttcataacc gtttccatct tcatcaaaac
    12241 ccgttttatc gaaccaggtt tttacgttgg cttcggtcat accccatcct tttttcacat
    12301 tggcaggatc atgaaattta gaaatagccc caccgggaac acgaatggta aatggctgat
    12361 tcatgtccac cagatattgt tcaacttcgg cattataatt ttcaaaaaat ccggagccat
    12421 tgaagcctaa attctgggct tttgcaactg ttccgaaaaa tgccaaaagg ataagtaatt
    12481 ttttcatgta aggttttttt agtaactctg cagtacaaaa gacaaatacc gtgcctacgc
    12541 accacaatgg caaacaaaaa taattcaatt tggctagtgt tactaatgct gcagttattg
    12601 ctgcataatg aagtgttggc acaaaccgct atcaccctgc cacagtgggg aaatgcctac
    12661 acgccgctgg ggagtaat
//
LOCUS       DAORKW010000039        12632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_76_length_12632_cov_5.188916, whole genome shotgun sequence.
ACCESSION   DAORKW010000039 DAORKW010000000
VERSION     DAORKW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12632
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76_length_12632_cov_5.188916"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..1135)
                     /locus_tag="PLB46_03045"
     CDS             complement(278..1135)
                     /locus_tag="PLB46_03045"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75520.1"
                     /translation="MKKILLLNFLITVSLLDVIAQTTSSTPYCTANYDGGIAGALTKY
                     ISKVEINEAGWVNATGTANPLPGFTYYNNLDPVPLSLNNNYTIKITHSGIAMHFVAVF
                     IDYNQDDDFADAGEVVNFVTANSPEFEFPGSPTTLEFTVPTTAVPGKTRMRVMVFEDD
                     DYTWNLNNSIPTPCTDYSMVNELDYGETEDYDVMISNTPVSTVELNNNSGVKMFPNPA
                     SDILNIVSNKNAIIYAEIVNTNGAVIKQFYPQNVSFEFSVKQFEKGLYIIRVYTDAIH
                     HTDNIITVM"
     gene            complement(1732..4611)
                     /locus_tag="PLB46_03050"
     CDS             complement(1732..4611)
                     /locus_tag="PLB46_03050"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF75521.1"
                     /translation="MKKLLRKLLVILLHVCFLLPANAQMFPSRQYTTEDELANSNVYD
                     ICHDNRGYLWFATERGVSRFDGYKFTNYLFKEGLGGSIVFGLAQDDKGTVYAATKYDG
                     VYRLDGNFVKSIEENKIIANEQIVTTSNYFYSLRDKRYITVVDLNGKQLQKIAFPENG
                     VIPNCIYKSPDGNVYSGTNSGIYELNGFNQPKKVFEEITLPVHSIYLQNEMWYFGCES
                     VVIATNGTTLNAIKLPEPGNIKRLLVDRNNNIWAATFPQNKLFLIANNRILDVSSKLG
                     LAGASVNKVMEDNEGNIWIATYGKGVFCIHHMFCTNYTSFDGLNNEYITAIEAHVDKS
                     VFIGSYNGLYYLDETGIHAKKLYDNSLEFIKELHGYDNHLFVTVAGIPEKKMYVGDPV
                     INNSQTTYLQASAILIDDDDLYYSHWDKKLWLTSRSQISQETARLVFYDSTAMWSRIN
                     KIFKDKKDNIWIGTTRGLYIIDPSGAYQKIDTGFFKTNITAFLYDATGTLLIGSDKGL
                     IKYKNNQWTTARDIKGKNLENITGLVKDAKERVWIGTLNGLFLLDKKSLIQFDTRNTL
                     LSDEINALEYDKKNNFVWVGTTYGLSSINLSLFDKTPVNPPAAIFKTLRATDSIYRDL
                     DINNTLTLPYTSKNFTVRFSAVHFSSPEGIKFLYKFDDGEWQPTTGRQIEFASMPYGK
                     HTLLLKSFGEQEIEGPVATLIINVETPFWATVWFKIMVGIAIGIAAYLILRKRFEAVR
                     KKQQERLELQSKIAELRHQALAASMNPHFIFNALNSIQHFINSHNTEEATDYLGKFAR
                     LIRMILDYGGKTFIPLQDELGGLNFSLELEKIRFGNKLTFTIDVDPTLVHQQPEIPNM
                     VIQPVVENALWHGLLPANRNGNLHISFAKIDNAIRVIVEDDGIGVNESKRRKKSGHNS
                     LGIQMIRERLDLLKKLSGYQATIMIQDKSDFNPPGQGTIVQINLA"
     gene            complement(4619..5359)
                     /locus_tag="PLB46_03055"
     CDS             complement(4619..5359)
                     /locus_tag="PLB46_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF75522.1"
                     /translation="MNRYTAILIDDEEPGRRNLLALLEKYCPEIEVIAEAANAAEAKE
                     KILSLGPNVLFLDINMPELNGFDLLESLNKKDFAVVFVTAHKDYGIQAVKAGAIDYIL
                     KPIVIKELQQAVNKIVAFYDQKRVELITDNQKPGRITLSHTGGFSVIEMKDIIRLEAE
                     SNYTRVYVTEKRSYFVSKPLKVFEDSIKDNIFFRVHRSYIINLNHVKEFLREDGGVIV
                     MNDDARIQLPKARYNDFIDAMRRLSIAI"
     gene            complement(5431..6939)
                     /locus_tag="PLB46_03060"
     CDS             complement(5431..6939)
                     /locus_tag="PLB46_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRF75523.1"
                     /translation="MYIIHSITSTVFKRQIARIQHAVNDPMRAQEAVFTSLMAGAAGT
                     EWGKKYDFASIKSIQDYRNRFPVQDYETLKPYIDRVMSGEQSVLWNTPISWFAKSSGT
                     TSDKSKFIPVSKMALDDCHFKGSRDVLAMYLHNNPSSRLFTGKTLLMGGSHQINQLNQ
                     HSKYGDVSAVMMQNMPLLSRYAMTPSLKIALMDEWESKIEAMADHTIDKNVTSIAGVP
                     TWTIVLIRRLFELTGKSDLKEIWPNLELYIHGGVSFKPYRSQFNDLITPGNMHYVETY
                     NASEGFFAFQDRLGSDEMLLMPDYGLFYEFIPMEEFDSEFPKTITLDEVELFKNYAVV
                     ISSNAGLWRYKIGDTVRFTSKNPYRIQVSGRVKHFINAFGEEVIVDNTDKAVAEACAQ
                     THATVVEYSAAPIYIEGNEKGGHEWIIEFGQQPDNLQQFIQILDKTLQSINSDYEAKR
                     HKDLALKMPVVHIAPKGAFYEWLKSKGKLGGQHKIPRLSNERTYIEEIKQFI"
     gene            complement(6984..7760)
                     /gene="lptB"
                     /locus_tag="PLB46_03065"
     CDS             complement(6984..7760)
                     /gene="lptB"
                     /locus_tag="PLB46_03065"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRF75524.1"
                     /translation="MILRSEKLIKKYKQRTVVNEVSVEVNQGEIVGLLGPNGAGKTTS
                     FYMIVGLIKPLSGNVYLDSKDITDYPMYKRAQLGIGYLPQEASVFRKMSVEDNIASVL
                     QMTNLKASEQKKKLESLIGEFGLTHIRKSNGDVLSGGERRRTEIARALATDPKFILLD
                     EPFAGIDPIAVEDIQLIVEKLKFKKIGVLITDHNVQETLSITDRAYLLFEGKILKQGS
                     AEDLAGDPQVRKVYLGQNFVLRKTRVSYAGSDAASEKDVE"
     gene            complement(7776..9473)
                     /gene="recJ"
                     /locus_tag="PLB46_03070"
     CDS             complement(7776..9473)
                     /gene="recJ"
                     /locus_tag="PLB46_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRF75525.1"
                     /translation="MEKRWVIKDADEDKVNSLQQELKVHPVVCKLLVQRGIYTFEAAR
                     KFFRPALTDLYNPFLMKDMQKAIDRINTAIANNERILIYGDYDVDGTTSVALAYSFFH
                     SIYKNIDYYIPDRYLEGYGISLKGIDHAKQNGCTLVIALDCGIKAVDKIAYANSLGVD
                     FIICDHHIPGDEVPAAFAVLDPHQIDCNYPYKELSGCGIGFKLIQAYAQHHQIAIENI
                     IPYLDLVVVSIASDIVPITDENRVLAFFGIEQLNKNPRPGLKALIDISGYEGQLNISN
                     IVFGLGPRINAAGRMDDANKAVRMLIAETVEMAKNGADVLHEKNVNRKEVDKMITGDA
                     LSMLHNDPDNDKYVSTVLCSDQWHKGVIGIVASRLIDHFYRPTILLTESEGMLYGSAR
                     SVKNFDIYEAIKACADLLEQFGGHMYAAGLSLKPENFEQFKKRFDDVVRANIKPWQLT
                     PEIEIDAALNLKDITPTFNNIIKQFAPFGPQNMDPIFMSEQLLNNGRSRIVGDNHLKL
                     GVKDNNRYNAEAIAFQKGAFFDAINNGARFDICYTIEENVYRDMKTIQLNVRDIKLN"
     gene            complement(9489..11114)
                     /locus_tag="PLB46_03075"
     CDS             complement(9489..11114)
                     /locus_tag="PLB46_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF75526.1"
                     /translation="MVWSATISAQTINQVEGEIILQLFPANSPQQVIAEINTSLQTDL
                     SIIKPVSKRLNIWLLHYDETLMNGDKILSALYMHPNVANVQYNHITAQRSVPNDPSFN
                     QQWNMFNDGTTGGISDADIDADLAWETTTGGVTILGDTIVVALIGEGANFIHEDLTYW
                     ENHNEIPENSIDDDGNGYIDDFHGWNSTADNDSVPERFHGTHVGGIAGAKGNNNIGVT
                     GVNWNLKIMPVYNVTNEADAVSSYSYALEMRELYESSNGEKGAYIVATNSSFGIDFAN
                     PADYPIWCAMFDSLGEVGILSVGSTANAYVNVDIVYDMPTACTSDYLITVTNTDKADN
                     LFGAGYGATTIDLGAPGTLIYSTILDNTYGVQSGTSMAAPHVTGTIALLFAAACPKFF
                     EDYQFDPANMALLLKQYILNGTDFAADLNGITVSNGRLNVNNALNNLLLTGYCNVGIN
                     DITTETNNMLVHPNPANNQLFFTFNDAINNEIIITIYNQTAQPVFSQTTNDADLMLNG
                     IPVHNLPNGWYAITATDIKTGKQFSTGCIVQHD"
     gene            complement(11196..11828)
                     /locus_tag="PLB46_03080"
     CDS             complement(11196..11828)
                     /locus_tag="PLB46_03080"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRF75527.1"
                     /translation="MLLFRNFLILSLLVVLASCSKQDTESPVISLVGAPTTSHFRGEP
                     YLDEGIVVIDNHTCNLENEIFTHNTVDENRYGAYTVTYSVQDEAGNATNAVRNVDVVL
                     PLTDYYLVDWHAFDTCTSGNYAYIGLVQDCDCPDFKVTVGNISNLGLSAIFALPVSGQ
                     FNQFLTLDTAKAGVTFSGIATMNPTADSLFWSYIISDSVSSDVCTSVWVK"
     gene            complement(11918..>12632)
                     /locus_tag="PLB46_03085"
     CDS             complement(11918..>12632)
                     /locus_tag="PLB46_03085"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRF75528.1"
                     /translation="KKLSMMTKFSVLTIVVVAIGTAIFTGCNTEDLTTPTIVLEGDSP
                     YYIVLGETWEEPGYTATDNEDGDITANVKVDSSAINLNEIGDYEVSYTVTDEAGNTGV
                     ETRIVRVVVGSANYLGSYQVNEICDIDGDGVLGEADVEFEINDYIVEVTAGIEVNELL
                     FFNFGNINEDVNVPVFFSGDLDDLLIVDNFNVEGSEIYIDADGVITTAVIGTIEFDLD
                     YTVTEGAAEVPCEATFVKL"
BASE COUNT         3910 a         2496 c         2159 g         4067 t
ORIGIN      
        1 aagatgatgc gaaagaggtt actaccagca tggaatgcgg tttaactatc cataacttta
       61 acgacataga agttggtgat attctggaag gatatgaaga ggtggaagta aaacgaacac
      121 tgtaaataat taccataaaa caaaaaggct gtctcacaag gacagccttt tttttgacca
      181 gcttaaaaca gttatttcgt ttttgttatg ttaaaataaa atatgcactc agctaaaatt
      241 caatcattaa tgtgatgttt atttaatatt gaaattttta cattaccgta ataatattat
      301 ccgtgtgatg aattgcatca gtataaactc gaataatata taaacccttc tcaaattgtt
      361 ttacagaaaa ttcaaatgat acattttgag gataaaactg tttaattact gcaccatttg
      421 tatttacaat ttctgcatat ataattgcat ttttattaga aacaatattt aatatatctg
      481 atgccggatt tggaaacatc ttaacaccgg aattattatt taattcaact gtagaaacgg
      541 gagtattaga tatcattaca tcataatctt ctgtttcacc ataatctaat tcgttcacca
      601 ttgaataatc ggtacatggg gtaggtatgc tattatttaa attccacgta tagtcatcat
      661 cttcaaatac cataacgcgc atgcgggttt tacccggaac agcagtggta ggtacagtaa
      721 attccaatgt tgtaggtgaa cccggaaatt caaattcagg agaattggct gtcacgaaat
      781 ttactacctc gcccgcatct gcaaaatcat catcctgatt ataatcaata aatacagcaa
      841 caaagtgcat ggcaatacct gaatgcgtaa ttttaatggt gtaattgtta ttcaagctta
      901 aaggaaccgg gtcaagatta ttataataag taaaccctgg taaaggattg gctgttccgg
      961 ttgcattaac ccagcctgct tcatttattt caactttaga aatgtatttg gtaagtgccc
     1021 ctgcaatacc accatcataa ttagcggtac agtaaggcgt agaagaagta gtttgagcaa
     1081 ttacatccaa taatgaaaca gtaataagaa aatttaagag gagtattttt ttcataatgt
     1141 aaaagtattt agaaatcaaa cttattctta tgctaattgt aaattgcatt tgaaactaaa
     1201 aatgacttct attttacaca atatcttctt aagtataatg aatcaattaa agcatcttta
     1261 aatgctaaca atgaaaggcc aataacttaa caaattagtt gcttgccgaa taggcataaa
     1321 aaaaggcgac atgagggagc cgccttttta gtctgtgtaa cgatggggtg catcattaca
     1381 catgaatcat caacccgaaa ttttcacccc caagtaaata acttggtttt catgggagtg
     1441 tttgcatgga gcaaaaaagt ttgtgtttcc gtggtgtgtg ttttcttaag ttgtgttttt
     1501 tgaggaattc gtgtgtgttt ctggttcaaa tagacagcaa ataaccctat tacacaaccc
     1561 actttgagtg aaatggtggt tttttcatgt gaaatgatgc tatgaattag cgaagtagca
     1621 acttcaacta gtgaaatatc cagaatccgg tgccaaatgc tgcataataa gttctgggta
     1681 aaacccggaa agtgttggtt ttgtcggaaa tgttggttta gcgactgcct atcaggcaag
     1741 attgatttgt actatggtgc cctgtcccgg cgggttaaag tccgatttgt cctgaatcat
     1801 gatagttgcc tggtaaccac ttagtttctt aagcaagtcg agacgttccc gtatcatttg
     1861 aatacccaga gaattgtgac cgcttttttt acgtcgtttg ctttcattta cccctatacc
     1921 gtcatcctct actattactc ggatggcatt atcaatttta gcaaaggaaa tatgcaggtt
     1981 gccattccgg ttggcaggta acaatccatg ccaaagtgca ttttccacta cgggttgaat
     2041 taccatattc ggaatttccg gttgctgatg aacaagcgtt ggatcgacat caattgtaaa
     2101 tgtcagttta ttgccaaaac gtattttttc gagttcgagt gaaaaattta aacctcccag
     2161 ttcgtcttgt aacggaataa atgttttacc gccataatcg agaatcatac gaataagccg
     2221 ggcaaatttt ccgaggtagt ctgttgcttc ttcggtatta tgcgaattaa taaaatgctg
     2281 aatagaattt aatgcattaa aaataaaatg cgggttcata ctggctgcta atgcctgatg
     2341 acgtaattcg gcaattttgg attgcagttc taatctttcc tgttgttttt tgcggactgc
     2401 ttcaaatcgt tttctcaaaa ttaaataagc agcaatacca attgcaatac ccaccattat
     2461 tttaaaccat accgtagccc aaaacggagt ttccacatta ataattaaag ttgcaacagg
     2521 tccttctatc tcctgttcac caaacgattt taataataaa gtatgtttac catacggcat
     2581 acttgcaaat tctatttggc gaccagttgt tggttgccat tcgccatcgt caaatttgta
     2641 taaaaattta ataccttccg gtgaagaaaa atgtactgcc gaaaaacgca ctgtgaagtt
     2701 tttagacgta tacggcaatg tgagtgtgtt attgatatct aaatcgcggt aaatggaatc
     2761 cgttgcgcgt aaagttttaa aaatggccgc aggaggatta acaggagttt tatcaaacaa
     2821 acttaaattg atactggaca aaccataggt tgttcccacc caaacaaaat tatttttttt
     2881 atcgtattcc agtgcattta tttcatcact taataaagtg tttcgcgtat cgaattgtat
     2941 caatgatttt ttatcgagga gaaacaaacc atttaatgtg ccaatccaaa ccctttcctt
     3001 ggcatcttta accagtccgg taatattttc aaggtttttc cctttaatat ctcgcgcagt
     3061 tgtccattga ttgtttttat atttaattag ccctttatcg ctgccaatta ataaggtacc
     3121 tgtggcgtca tataaaaagg ctgtgatatt tgttttaaaa aaaccggtat caattttttg
     3181 ataggcgccg ctagggtcaa taatatataa acctcttgta gttcctatcc agatattgtc
     3241 ctttttatct ttaaatattt tatttatcct cgaccacatt gcggtggaat cataaaatac
     3301 taatcgtgct gtttcctgtg aaatttggct cctgctggtc agccacaatt ttttatccca
     3361 atggctgtaa tacaaatcat catcatctat taaaatggca ctagcctgta aataggtggt
     3421 ttgagaatta ttaataacag gatcgccaac atacattttt ttctccggta taccggccac
     3481 agtaacaaac aaatggttgt catacccatg cagctcttta atgaattcta atgaattgtc
     3541 atataatttt ttggcatgta tgccggtttc atctaaataa tataaaccgt tatagcttcc
     3601 tataaatacc gatttatcta catgtgcttc tatggctgtt atatattcat tatttaatcc
     3661 atcgaaagaa gtataattgg tgcaaaacat atggtgaata caaaacactc cttttccgta
     3721 agtagcaatc caaatatttc cctcattgtc ttccattact ttgtttacag aagcgcctgc
     3781 cagaccaagt ttggacgaaa catccaaaat cctgttattc gcaatcagga ataatttatt
     3841 ttgtggaaag gtagcagccc atatattatt atttctgtct actaataaac gtttgatgtt
     3901 tccgggttcg ggcaatttaa ttgcattaag tgttgtgccg ttagttgcta tcacaacact
     3961 ttcacaacca aaataccaca tctcattttg cagataaata ctatgcaccg gaagtgtaat
     4021 ttcttcaaat acttttttag gttgattaaa accgttgagt tcatatattc ctgaattggt
     4081 tccggagtac acattgccgt caggtgattt gtaaatacaa ttgggaatga ctccgttttc
     4141 aggaaaagca attttttgaa gttgtttgcc atttaaatca acgactgtaa tgtaccgctt
     4201 atcgcgcagt gaataaaaat aattggacgt ggttactatt tgttcgttgg caattatttt
     4261 attttcctca atagatttca cgaaattgcc atcgaggcgg tatacgccat catatttagt
     4321 tgcagcatac acagttcctt tatcatcctg tgcaagacca aaaacaatag acccgcccaa
     4381 accttcttta aacaggtagt tggtaaactt ataaccatcg aacctgctta caccgcgttc
     4441 ggtagcaaac cataaataac cacggttatc gtgacaaata tcatacacat tactattggc
     4501 taattcatct tcagtagtat attgccttga aggaaacatt tgcgcatttg cgggaagcaa
     4561 aaaacacacg tgcaataata ttaccagtaa tttgcgcaac aattttttca tgagtggttt
     4621 aaatggcaat acttagtcgg cgcattgcat caataaaatc gttataacgc gcttttggta
     4681 gttgaattcg ggcatcgtcg ttcattacaa ttacaccacc atcttcacgc aaaaattctt
     4741 tcacatgatt taagttgata atataagaac gatgcacgcg gaaaaagata ttatccttga
     4801 tactgtcttc aaaaactttt aaaggtttag aaacgaagta acttcttttt tcggtaacat
     4861 aaacacgcgt ataattactt tccgcttcga ggcgaattat atctttcatt tcaattacac
     4921 taaatcctcc tgtatggctg agtgtaatac gacccggttt ttgattatcc gtaataagtt
     4981 caacacgttt ttggtcgtaa aatgccacaa ttttattaac ggcttgttgt aactctttaa
     5041 ttacaattgg ttttaagata taatcaattg cacctgcttt tacagcttga ataccataat
     5101 ctttatgtgc tgtaacaaac acaacagcaa aatctttttt attgagtgat tccaataaat
     5161 cgaacccatt tagttcgggc atattaatat ccagaaacaa cacattaggt ccgagcgata
     5221 aaattttttc ttttgcttca gcagcatttg cagcttcggc aattacttca atttcagggc
     5281 aatatttttc taacaacgcg agtaaatttc gtctacccgg ctcctcgtcg tcgattaaaa
     5341 tggctgtgta acggttcacg gtaataggtt taatttgata gctaatgcaa tcaaagttaa
     5401 ctaattatac caatagcaaa aacgtggaaa tcagataaac tgcttaattt cttcaatata
     5461 agtgcgttca ttactcaggc gaggtatttt gtgctgtcca ccgagtttac ctttcgattt
     5521 gagccattcg taaaatgcac ctttaggagc aatatgcact accggcattt ttagggcaag
     5581 gtctttatgt cttttagctt cgtaatcgct atttatagat tgcaatgttt tatctagaat
     5641 ttgaataaac tgctggagat tatccggttg ttgtccgaat tcaattatcc attcatgacc
     5701 acctttttcg ttgccttcaa tgtatatggg agcggcagaa tattccacaa cagtagcatg
     5761 tgtttgtgcg cacgcttcag ccactgcctt atctgtattg tcaacaatta cttcttcacc
     5821 aaaagcatta ataaaatgtt ttacgcgacc tgaaacctgt atgcgataag ggtttttaga
     5881 agtaaatcga acggtatcac caattttata acgccaaagc ccggcattgg atgaaattac
     5941 cacagcataa tttttgaaca gctcaacttc atctaacgta attgttttag gaaattcact
     6001 gtcaaattct tccattggaa taaactcata aaaaaggcca taatccggca tcaaaagcat
     6061 ctcatcagac ccaagtctgt cctgaaaagc aaaaaatcct tcacttgcat tataggtttc
     6121 aacataatgc atattgccgg gtgtaataag gtcgttaaat tggcttcgat aaggtttaaa
     6181 gcttacacca ccgtgaatat ataactccag atttggccaa atttctttta aatctgactt
     6241 accggtaagc tcgaataacc tgcgtattaa tacgatggtc caggtaggta cacctgctat
     6301 actggtaaca tttttatcga tggtatggtc ggccattgct tcaatcttgc tttcccactc
     6361 atccattaat gcaattttta acgaaggcgt catagcataa cgggaaagaa ggggcatatt
     6421 ttgcatcata acagcactta catcgccgta tttggaatgc tgattgagtt ggttgatttg
     6481 atggctgcca cccataagca gggttttccc ggtaaataat cgggaggaag ggttgttgtg
     6541 caaatacata gccaacacat cgcggctgcc tttaaaatgg caatcgtcca atgccatttt
     6601 gctgacaggg ataaatttac ttttatcgct ggtggttccc gatgatttgg cgaaccagct
     6661 gatgggcgta ttccaaagaa cagattgctc gccggacatt actctgtcta tatatggctt
     6721 tagggtttcg tagtcctgca caggaaaacg attgcgataa tcctgaatag attttatgga
     6781 agcaaaatca tattttttcc cccactcggt tccggcagct ccggccataa gtgatgtgaa
     6841 cacagcttcc tgtgcacgca tagggtcgtt aactgcatgc tgaatgcgcg caatttgcct
     6901 tttaaaaacg gtggaagtta tggaatgaat aatgtacatc cgcgatacaa aaataaaacc
     6961 ctgccttcac ataaaggtat agattattcc acatcctttt cggaagccgc atcggagccg
     7021 gcataagaca cccttgtttt gcgtaaaaca aagttttggc ctaagtacac tttacgaact
     7081 tgtgggtcac cggcaaggtc ttcggcagaa ccttgtttaa gtatttttcc ttcaaataat
     7141 aaataggctc tatcggtaat agataacgtt tcctgcacat tatggtcggt aatgagcaca
     7201 cctatttttt tgaattttaa tttttctaca atcaattgaa tgtcttccac cgcaatcggg
     7261 tcgataccgg cgaaaggttc atctaataaa ataaatttgg gatcggtagc taatgctctg
     7321 gcaatttctg ttcttcttct ttcaccacca cttaatacat caccattact tttgcgaatg
     7381 tgtgttagtc cgaattctcc aattaatgat tccagttttt tcttctgctc actggctttt
     7441 aaattagtca tttgcagtac gctcgcaata ttatcttcca ccgacatttt tctaaataca
     7501 cttgcttctt gcggcaaata accaattcct aactgagcac gtttatacat gggataatcg
     7561 gtaatatctt ttgagtcgag atatacatta cccgaaagtg gtttgattaa tcccacaatc
     7621 atataaaatg aagtggtttt tcccgcacca ttaggtccca gcaaaccaac aatttctccc
     7681 tggtttactt caacgctaac ttcatttaca acagtgcgtt gtttatattt tttaatgagt
     7741 ttttctgaac gtaaaatcac aaatgagtat ttaaattaat tcaactttat atcacgaacg
     7801 tttaattgga tagttttcat atcccggtaa acattttctt caatagtata acaaatatcg
     7861 aaccgagctc cattgttaat ggcatcaaaa aatgcacctt tttgaaaagc gatagcttca
     7921 gcattgtaac ggttattgtc ttttacacca agttttaaat ggttatcacc aacaattctg
     7981 gaccttccgt tatttaataa ctgttcactc ataaaaatcg gatccatatt ttgtggaccg
     8041 aatggtgcaa attgtttaat gatattatta aaagttggtg ttatgtcttt taaattgagc
     8101 gcagcatcta tttcaatttc cggggtaagc tgccatggtt taatattagc cctcacaaca
     8161 tcatcaaaac gttttttaaa ttgctcaaaa ttttctggct ttagcgataa acctgctgca
     8221 tacatatgac cgccaaattg ttcgagcaaa tcggcacagg ctttaatggc ttcataaatg
     8281 tcgaaatttt ttacggaacg tgcgctgcca tacaacatcc cttccgactc agtgagtaaa
     8341 atagtagggc gataaaaatg gtcaattaat cgagatgcca caattccgat aacgccttta
     8401 tgccattggt cactacacag tacagtagaa acatatttgt cgttatccgg gtcgttgtgc
     8461 aacatactta atgcatcacc cgtaatcatt ttatctactt ccttgcggtt gacatttttt
     8521 tcatgtaaaa catcggcacc attttttgcc atttcaacag tttcggcaat taacatgcgg
     8581 actgctttat tagcatcatc catccttccg gcagcattaa tacgtggccc caatccgaaa
     8641 acaatattcg aaatatttaa ttgcccttcg tatcctgaaa tatctatcaa agcttttaaa
     8701 cccggtcgcg gatttttatt taattgttcg atgccaaaaa atgccagcac tctgttttca
     8761 tcggttatag gcactatatc actggcaata ctcaccacta ctaaatccaa atacggaata
     8821 atattttcga tggcaatttg gtgatgttgt gcataggcct gaattaattt aaacccaata
     8881 ccgcaaccac ttaattcttt gtaagggtaa ttacaatcaa tttgatgagg gtccaacacg
     8941 gcaaaagctg cgggcacttc atcgcccgga atatggtggt cacaaataat aaaatcgaca
     9001 cctaaactat tggcgtatgc aattttatca acggctttaa taccacaatc taatgcaata
     9061 actaatgtgc aaccgttttg ttttgcatgg tcaatacctt tcaaagaaat accataccct
     9121 tctaaatatc tgtcggggat ataataatcg atatttttat aaatggaatg aaaaaacgaa
     9181 taagccaaag ctactgaagt agtgccatct acatcgtaat cgccatatat taaaattcgt
     9241 tcattatttg caattgctgt atttattctg tcaattgctt tttgcatatc cttcattaaa
     9301 aaaggattat acaaatcagt taatgcaggt ctgaaaaatt ttcgtgcagc ttcaaaggta
     9361 taaatacctc tttgcactaa caatttacac accacaggat gcacttttaa ttcctgttgc
     9421 agcgaattca ctttgtcttc atccgcgtct ttaatcaccc atcgtttctc catcgcaaaa
     9481 tgtttcactt aatcatgttg aactatacag ccggttgaaa attgtttacc ggtttttata
     9541 tctgtagccg taattgcata ccatccattt ggtaaattat gcaccggaat accatttaac
     9601 attaaatcag catcgtttgt tgtttgacta aacaccggtt gtgctgtttg attgtaaata
     9661 gtaataatta tttcattatt tatagcatcg ttaaatgtaa aaaataattg attgttagca
     9721 ggattaggat gcacaagcat attatttgtt tccgttgtaa tgtcatttat acctacatta
     9781 caataaccgg ttaataataa attattgagt gcattattta catttaatcg tccgttgctt
     9841 actgtgatac catttaaatc tgcggcaaaa tcggtgccgt ttaaaatata ttgtttcaac
     9901 aacagagcca tattagccgg atcaaactga taatcttcaa aaaatttagg acaagccgct
     9961 gcaaataaca atgctatagt tcctgtaaca tgtggtgctg ccatactggt gccggattga
    10021 accccatacg tattatctaa aatagtggaa taaatcaatg ttcccggagc tcccaagtca
    10081 atagtagttg caccgtatcc tgcgccaaat aaattatctg ctttatcggt attggtaaca
    10141 gtaattaaat aatcagaagt acatgctgtt ggcatatcat aaacgatatc tacattcaca
    10201 tacgcatttg ctgtagagcc aacacttaat atacctactt ccccaagtga atcgaacatg
    10261 gcacaccaaa tgggataatc agcggggttg gcaaaatcaa ttccaaatga tgaattagtt
    10321 gctacaatat atgcaccttt ttcgccattg gaactttcat ataattcacg catttctaat
    10381 gcataactgt acgaagaaac agcatcggct tcattggtta cattatacac aggcattatt
    10441 ttcaaattcc aattaactcc tgttactccg atattattat ttccttttgc gccggcaatt
    10501 ccgccaacat gtgtaccatg aaatctttca ggaacactgt cgttatcagc ggtagaattc
    10561 caaccatgaa aatcgtcaat atatccattg ccatcatcat cgattgaatt ttcaggaatc
    10621 tcattatgat tttcccaata ggttaaatct tcatgtataa aattagcgcc ttctccaatt
    10681 aatgctacaa caatcgtatc gcctaaaatt gtaacaccgc cggtagttgt ctcccatgcc
    10741 aaatcggcat cgatatcggc atcactaatt ccacctgttg taccatcgtt aaacatattc
    10801 cattgctgat taaatgaagg gtcgtttggt acagagcgtt gagcagttat atgattatat
    10861 tgcacattcg caacatttgg atgcatgtat aatgccgata atattttatc gccattcatt
    10921 aaagtttcat cataatgcaa cagccagatg tttaatcttt tggaaacagg tttaattatc
    10981 gatagatctg tttgtaatga cgtatttatt tcagcaatta cctgttgagg tgaattagcc
    11041 gggaataatt gtaaaattat ttcaccttcc acctgattaa ttgtttgtgc agaaatagtt
    11101 gcactccata ccatcaggaa taatacacat tttattttac ctaaaaacat atctcttagt
    11161 aaatatacat aacatcaatc attcgaacaa agttgttatt ttacccaaac tgatgtgcaa
    11221 acatcgctgg aaacagagtc ggaaattata taactccaaa acaacgagtc ggctgtcggg
    11281 ttcatagtgg caataccgga aaaagtgacg cctgcttttg cggtatcgag cgttaaaaac
    11341 tgattaaact gtccggatac cggtaaagca aaaatcgccg aaagtcctaa attgctgata
    11401 ttacctacag taaccttaaa atcagggcaa tcgcaatcct gaacaagtcc gatataagcg
    11461 taattacctg atgtacacgt atcaaaagca tgccagtcca ccaaataata atcggttaaa
    11521 ggcaaaacaa cgtcgacatt tctgactgca tttgtcgcat ttccggcttc atcctgcact
    11581 gaataggtta cagtataagc gccgtagcga ttttcatcta cggtattatg tgtaaaaatc
    11641 tcgttctcca aattgcaggt atggttgtca ataactacaa ttccctcatc tagatagggt
    11701 tcacctctaa aatgggaagt agtgggcgct cctaccaggc ttattacagg cgattcggta
    11761 tcctgtttgc tgcaggaagc caaaactaca agtaaactga gtattaaaaa gttgcgaaat
    11821 agtaacatca acgggtaata aatgggcaac taaggtaata atattaaaac aaaagccccg
    11881 aaatcagtga tttcagggtt tttaaatgca ttttcaccta taatttcacg aaagttgctt
    11941 cacaaggcac ctccgcagca ccttcggtaa cagtgtagtc caaatcaaat tcaatagttc
    12001 ctattacggc tgtggtaata acaccatctg catcgatata aatttctgag ccttccacat
    12061 taaaattatc tacaatcagc aagtcatcaa ggtcgccact gaaaaacaca ggaacattta
    12121 catcttcgtt aatattgcca aaattgaaga aaagcagttc attaacttcg atacccgctg
    12181 taacctctac aatataatcg ttgatttcaa actcaacatc tgcttcgccc agcactccat
    12241 ccccatctat atcacaaatt tcattgacct gataactgcc taaatagtta gcacttccta
    12301 ctaccacgcg cacaatgcgg gtttcaacac ctgtattgcc agcttcatcg gtaacggtat
    12361 aacttacttc gtaatcacca atttcattta aattgatggc tgaactatcc acctttacgt
    12421 tggcagtaat atcgccatct tcattgtcag tagcagtata accaggctct tcccaggttt
    12481 caccaagcac aatgtagtaa ggagaatccc cctctaaaac aatagttggc gtagtaaggt
    12541 cttcggtgtt acacccagta aaaatcgcag ttccaatagc aacaaccaca atggtcaaca
    12601 ctgaaaattt agtcatcata gatagttttt tt
//
LOCUS       DAORKW010000040        12632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_77_length_12632_cov_5.041981, whole genome shotgun sequence.
ACCESSION   DAORKW010000040 DAORKW010000000
VERSION     DAORKW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12632
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77_length_12632_cov_5.041981"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLB46_03090"
     CDS             <1..552
                     /locus_tag="PLB46_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HRF75529.1"
                     /translation="VAVMFTKNVTMMNNTFEDNWGDAAYGLLLKEISDSNISGNRFIN
                     NTTGIYMEGTSRIWLDKNLFRENGWGIKIMASCMDNSLRKNDFYSNSFDVSTNGNVVL
                     NHFEGNYWDRYHGYDLNKDGIGDVTYHPLSMFGVVVEKMPSAMLLHRSFFITLLDKSE
                     KVLPSLTPSNFEDKFPNIKPSLI"
     gene            549..1253
                     /locus_tag="PLB46_03095"
     CDS             549..1253
                     /locus_tag="PLB46_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF75530.1"
                     /translation="MIKIRKLKKSFGKLEVLKSIDLDLSQSECIGLIGPNGCGKTTLF
                     KSILNMVIPTQGEILVNNKSIYGNDNYRENIGFMPQIGRYPENMSIGQVLGTLKSLRP
                     QTKNFDMDLFNQYEIPRLLNKKMGTLSGGTRQKISAVLAFMFNPAILMLDEPTAGLDP
                     LSAEILKDKIQLEKNKGKLILITSHLLSELDDMVSEVIFMQEGNVTFHKKYATLMLET
                     KENTLAKAITHVLKAT"
     gene            1255..1755
                     /locus_tag="PLB46_03100"
     CDS             1255..1755
                     /locus_tag="PLB46_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75531.1"
                     /translation="MSKIGQLVLIDIFKNKIVLIYAVVLLIITWSVFMLEDTSGKGLL
                     TLLNVILLVVPLMSLLFSTIYLYNSAEFIELLVSQPVKRQQIWLSLYFALSLSLAGAF
                     VIATIIPLLLFCNLAQALMMTITGFAVTLVFISLGFVASIISRDKAKGIGIAILMWLY
                     FALLFD"
     gene            2019..2315
                     /locus_tag="PLB46_03105"
     CDS             2019..2315
                     /locus_tag="PLB46_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75532.1"
                     /translation="MKLGNVKGKLESGINPVVINFVKEENCKVFAIFFKQGMVLTDHK
                     TNRPAILYVLTGKVQFKTADQMLEVGCFEEIDIPVGVIHQVVCLEEATCLLIQK"
     gene            2323..2694
                     /locus_tag="PLB46_03110"
     CDS             2323..2694
                     /locus_tag="PLB46_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRF75533.1"
                     /translation="MKNDITNRGDIEMLVNAFYDKVKTDDKIGLFFTDVIHVNWEQHL
                     PRMYDFWDNVLFQTGSYIGNPMQRHRQIHLQHPIQNEQFIHWVNLFHATIDEFFSGKN
                     AELLKSRAASIAAIMQVRVNQ"
     gene            2699..3139
                     /locus_tag="PLB46_03115"
     CDS             2699..3139
                     /locus_tag="PLB46_03115"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRF75534.1"
                     /translation="MYTVVFLLHLLAACIWVGGHLILAITILPHALKTKSVETLLNFE
                     NKFEKLGIPALIVQIISGFYLADWHHVKMRTWLQFDNPVETVISIKLICLVATLLLGA
                     HARLKLIPNLSPKSLNALAIHITLVTLIGVTMLILGTLVRFGGL"
     gene            complement(3192..3752)
                     /locus_tag="PLB46_03120"
     CDS             complement(3192..3752)
                     /locus_tag="PLB46_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRF75535.1"
                     /translation="MKTRIICLLFTSSLFIISCKNEPKQTSPTTDSETVQPTGQSGVK
                     DDVSNPNIVQVASGSPDHTTLVAAVKAADLVDALSNTGPFTVYAPTNAAFEKLPKGTV
                     EGLLEPSKKADLQAILEYHTYVGVLKTAYLSDGQEFEQVSGQKITITLRDGKTYVNGT
                     AEIVASIPTSNGIIHVINEVLLPPSK"
     gene            complement(3810..5045)
                     /locus_tag="PLB46_03125"
     CDS             complement(3810..5045)
                     /locus_tag="PLB46_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75536.1"
                     /translation="MYTINNKFWVKLGLFNFSIVAFVGFIMRYKIGYSFPFFDQKNLL
                     HAHSHFAFSGWVSHILMLFITLAIAQNISPKRLRYYQICILLNIICAFGMLIGFTING
                     YNTFTIIISSISLFTSIAYAIITLSDLLQNKTIPQRMWFIFANCMQIISAAGTASLVL
                     MMAKHQIEQERYLASIYWYLHFQYNGWFFFACMGLFFTYIKKLLPQAEIPSAIFNYFA
                     ISCIPAFGLSVLWLNLPILVYITIMLAAICQICGWLWLVLFLKNHKVISIFSKKDLLI
                     KLLFITIFCATTVKLFLQLFSVIPSISEMAFGFRPIVIAYLHLVLLAITSLFLLTYAY
                     AMKFINHNIYTKIALILIAAGVLLNECALGIQGIASLSYTPVPHIDQILVIISGILVV
                     GILAILLAQLKSSKQNESI"
     gene            complement(5046..5642)
                     /locus_tag="PLB46_03130"
     CDS             complement(5046..5642)
                     /locus_tag="PLB46_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF75537.1"
                     /translation="MSSIDTNLLISWGATYKKYKKGEYIFYEGDEALFYYQIESGELR
                     MSNMNDSGKEYIQGMFSNGESFGEPPLFLNEKYPASAIANEDLVVLRISKEYFFKILR
                     ENTELQFTFLKILAKKVYSKAITAKSVINHTPEERIMAFLCDYKRKLKEHENHIQIPF
                     TRQEIANYTGLRVETVIRTLSVMKSKNMIIIKDRKLFF"
     gene            5943..6788
                     /locus_tag="PLB46_03135"
     CDS             5943..6788
                     /locus_tag="PLB46_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRF75538.1"
                     /translation="MADIHQLKRICSQVRRDIVREVYQCQSGHPGGSLGCTEFFVALY
                     FDVMNHNNNFNMNGEGEDLFFLSNGHISPVWYSVLARSGYFPLEELTTFRKLDSRLQG
                     HPTTAEHLPGIRIASGSLGQGMSVGIGAAQAKKLNGDTHLVYTLHGDGELQEGQIWEA
                     AMYAAHVGVDNIICTVDWNGQQIDGPTKKVMDLGDLKAKFDAFGWDTLVCNDGNDMEQ
                     LLLALNTAKSKTGKGKPVMILMKTAMGKGVDFMEGSHEWHGIAPNKDQLEKAMAQLEE
                     TLGDY"
     gene            6861..8888
                     /locus_tag="PLB46_03140"
     CDS             6861..8888
                     /locus_tag="PLB46_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75539.1"
                     /translation="MKKAKQSGRLVHSAKLKNYSALSASFIALAAPVLSQVVYTDVNP
                     DINVFLDEITLDLDNNGTNDFKLVFVSETFATFAPAELKLRINPLGENKIAYSFQTIP
                     VTYSGTPWYTYNSGATQIVFSNVPLLNESAVIDEAMDFSLDKAALFEKLYFYIYSSYD
                     LQAFEGGWWNGTENHFAAFQLDIDGTYHFGWMRLNVSVETGITLLDYAFESLPDSPIT
                     TAINNYSISWINVADAGITASPEDLTFSFNAAGDEEDIDAYRVICAKAGTSISVAEAN
                     GLPADRYVSIIPDGSASYNGNFGTIIVDSDGDIITTNQEYQLWVLHELNAATGYLNTL
                     SIPSNTIQLVDTVAIVTDLSITDIDNNGNAADIRIDFDAPAVEQGIVAYRIYITQKDA
                     ADIFTLSDALVLGADLYIEVLPGDVSYSIIPTPSLLNTEGNIIEPDKYKAFVVSIPDG
                     TTVEHAAMTLASNVISLEQPTSVVENILLEDIADEGNGADIKVTYFIPSYEQTIESYH
                     IFFVDFATAFDFDLSSALASTNYIEITPTGSDITVTGDATTKDSNGNLITWGVPYYAY
                     VLALASDYGIDDTLSLPSNQVILNYPVIIGIQDAAQQIPQIFAHGNQVVIQLPNDNPA
                     TVNIYNTLGQNIFNRVITSSEKITVDLPEGNYFVSITQLESIYTRQVFVTE"
     gene            9094..10455
                     /locus_tag="PLB46_03145"
     CDS             9094..10455
                     /locus_tag="PLB46_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF75540.1"
                     /translation="MRNIIVVCLLFLGFSLSAQTTTAGSAKRILILLDGSGSMLDPWK
                     GTNKWEVAKKLVVKTIDSIQKEDPEVEIGIRVFGHQSPRAAHDCKDSKLEVSISKNSG
                     YNVKNALNNIVPKGYTPIAYSLFLSAGDFISTEGTNSIILITDGIENCEGDPCASSQA
                     LRDKKITLKPFVIGLGLAETDKKQFDCIGSYYDAGDEKSFSNAMSIVVSQALNITTTQ
                     INLLDAFGLPVEKNIEITLYDHATGEVRYNYVHTPDSRNQPDTLFLNPIGKYDIVVHT
                     FPIVTLNDVELTPGKHNIIGIDVPLGNLIISEGQSTSFSPKQCVVRDNKTGEIVYAQN
                     FNTKERYIAGVYDIDILTVPRLHYEDFVIKGGVDNKIDIPLPGTLNISTTENKVYSIY
                     LVKEGKLEKVYESNLNANNEMVQLLPGDYKIFSRSNIKKYSANTKDIAVTIKSAKTTI
                     IKI"
     gene            10515..11825
                     /locus_tag="PLB46_03150"
     CDS             10515..11825
                     /locus_tag="PLB46_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF75541.1"
                     /translation="MNVIAQTTQVTRILFVVDASRSMSQTWDRSAKMAAARAVVNGIA
                     DSLNDNPNIQMAMRVYGHQSPQPLNDCEDSKLEIGFRTKNGLSIKNRLDDIRPNGVTP
                     IAYSMEQSLTDFGPDAKNYRNILILVSDGFESCGKNPCEVVQRLRNMGVITKSYVIGI
                     GIDATEFTQFSCMGEFMNIESEQKTEQVIDATIAKIFNSVYVKVDLLDTYNQPEETDV
                     LMTFYDATSDVQKFNYYHTINPKGNPDTITLDPALNYDMQVHTTPELMKKDIELTPGK
                     INTITQPAPQGYLVVDVRNEKFVATLNCIIKKDNKIVTWEQTNKPRKLLVGNYDVEIL
                     TLPIITVKNVKVEQDKTTTLEIAAPGFATISKTESVSGGIYEYRENKLVEIYELNESS
                     LKETLTIQPGKYKIIYRYLSKKDMDATKEVDFEITSGASFTIRL"
     gene            11891..>12632
                     /locus_tag="PLB46_03155"
     CDS             11891..>12632
                     /locus_tag="PLB46_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRF75542.1"
                     /translation="MINVDYTNQKDTRSGFGVGILEAGKANPQVVALCADLIGSLKLD
                     AFIKEFPDRFFQCGIAEANMTGIAAGLTIGGKIPFACTFANFATGRVYDQIRQSIAYS
                     NKNVKIAASHAGVTLGEDGATHQILEDLGLMQMLPNFVVINPCDYNQTKAATIAAAEY
                     DGPVYLRFGRPKWPIFYPEQEKFEIGKAWKIQDGKDVSIFATGHLVLKAIEATKILEE
                     KGISVDLINIHTIKPLDIPAVIASVSKTK"
BASE COUNT         4213 a         2022 c         2373 g         4024 t
ORIGIN      
        1 gttgcagtta tgtttacaaa gaacgtaacg atgatgaata atacttttga agacaattgg
       61 ggtgatgcgg cttatggttt gttattaaaa gaaatttcag atagtaatat ttcaggcaat
      121 agattcataa ataatactac tggtatttac atggaaggga caagtagaat ttggttggat
      181 aaaaatttat tcagggaaaa tggatgggga attaaaatta tggccagttg tatggataat
      241 tcgctccgca agaatgattt ttattccaat tcatttgatg tttcaactaa tggaaatgtt
      301 gtgcttaatc actttgaggg caattattgg gatagatacc acggctacga tttaaataag
      361 gatgggatag gtgatgttac ttatcatcca ttgagtatgt ttggtgtagt agtagaaaaa
      421 atgccatcgg ctatgttatt acatagaagt ttttttataa cattattaga caagtcggaa
      481 aaagttttac cttctcttac gccatctaat tttgaagata agtttcctaa tattaaacca
      541 tcgttaatat gattaaaata cgtaaactta aaaagagctt cggaaaattg gaggtgctca
      601 aatctattga tcttgacctg tcacaatcag aatgcattgg tttaattgga ccaaatggtt
      661 gcggcaaaac cactctgttt aagtctatac ttaatatggt aatacctacg caaggtgaaa
      721 tcttagttaa caataagtca atttacggta atgataatta tcgtgaaaat attggattta
      781 tgcctcaaat agggcgttat cctgaaaata tgtcaatcgg gcaggtttta ggtactttaa
      841 agtcgttgag accacaaaca aaaaactttg acatggattt atttaatcag tatgaaatac
      901 cacggctatt aaacaaaaaa atggggacat taagtggtgg aacaaggcaa aaaataagtg
      961 cagtattagc atttatgttt aaccctgcaa tattaatgtt agatgaacca acagcaggat
     1021 tagacccatt atcggctgaa attttgaaag ataaaattca acttgaaaaa aataaaggaa
     1081 agctaatttt aattacttct catttattaa gtgaattaga tgacatggtt tcagaagtaa
     1141 tatttatgca ggagggcaat gttacatttc ataaaaaata tgcaacattg atgttagaaa
     1201 ctaaggaaaa tacattggct aaagctatca cacatgtttt aaaagcaacc tgatatgagt
     1261 aaaattgggc aacttgtgtt aatagatatt tttaaaaaca agattgtatt aatttacgct
     1321 gttgttttgt tgattattac atggagtgtt ttcatgttag aagatacttc cggcaaaggg
     1381 ttacttactt tactaaatgt aatcttgcta gttgtgcctt tgatgtcctt attgttttca
     1441 actatatatt tatacaacag tgctgaattt attgaattat tagttagtca gccggttaaa
     1501 cgccagcaaa tatggctgag tttgtacttt gcattgtcac ttagtttggc aggtgcattt
     1561 gtaattgcta caattattcc actattgttg ttttgtaatt tagcacaagc attaatgatg
     1621 acaattactg ggtttgctgt caccttggta tttatttcac tcgggtttgt tgcttccata
     1681 attagcagag ataaggctaa aggcattgga atcgcaattc ttatgtggtt gtatttcgca
     1741 ttgttatttg attgacttgt attattcctg ctgttccagt ttgctgaata cccaattgaa
     1801 aaacccatgg tcattttatc catcttctca ccactagatt tggctcgcat caataattta
     1861 atgcaaatgg atgccgccgc attaatgggt tataccgggg caatatttaa acaatatttt
     1921 gggtctggcc tgggcttatt tataacgggt tttgctatgt tattgtggac tatcgtccca
     1981 ttatttattt caacacgaat atttaaaagg agagatttat gaaattagga aatgttaaag
     2041 gaaagttgga aagtggaatt aatccggtag tgataaattt cgttaaagaa gaaaactgca
     2101 aggtgtttgc aatttttttc aaacaaggga tggtgctaac tgatcataaa actaaccgcc
     2161 ctgcaatttt gtatgtatta accggtaaag ttcagttcaa aacagctgac cagatgttgg
     2221 aagttggttg ttttgaggaa attgatatac ctgttggtgt tatacatcaa gttgtttgct
     2281 tagaggaagc aacatgtttg ttaattcaaa aataaattaa ttatgaaaaa tgacattact
     2341 aatcgaggcg acatcgaaat gcttgtaaat gctttttatg acaaagttaa aactgatgat
     2401 aaaattggcc tcttttttac agatgtaatt catgtaaatt gggaacagca tttgccaaga
     2461 atgtatgatt tttgggataa tgtattattt caaacggggt cttatattgg taacccaatg
     2521 cagcggcaca gacaaattca cttacaacat cctattcaaa atgaacaatt tatccattgg
     2581 gtaaatttat ttcatgcaac tattgatgaa tttttttcgg gtaaaaatgc ggaattgtta
     2641 aaaagtagag cagccagtat tgctgctatc atgcaagttc gtgtaaatca ataaaattat
     2701 gtatactgtt gtctttttat tacatttatt ggcggcctgt atctgggttg ggggtcactt
     2761 gattctggca atcactattt tgccacatgc cctaaaaaca aaatctgtag agactttact
     2821 aaattttgaa aataaatttg aaaagcttgg tatacctgca ttaattgtcc aaattatttc
     2881 aggcttctat cttgctgatt ggcatcatgt aaaaatgagg acatggctcc aatttgacaa
     2941 cccggttgaa actgtaattt caattaaact tatttgtttg gttgcaacgt tgttacttgg
     3001 ggcacatgca agattaaaat taatcccaaa tttgtctcca aaatcattaa atgcattagc
     3061 aatccatatt acgttagtta ccttgattgg ggtaacaatg ttaatattgg gcactttagt
     3121 taggtttggt ggattataaa aaaaatggtg cgaaattaat tttcgcacca tttcaaacaa
     3181 aactgaaaat attattttga aggcgggaga agcacctcat taattacatg tattattccg
     3241 tttgatgtag gtatgctggc aacaatttca gctgtgccgt ttacatatgt tttaccatca
     3301 cgcaaagtaa tggtaatttt ttggccacta acttgttcaa actcttgacc atcacttaaa
     3361 tacgcagttt ttaaaacacc aacataggtg tgatactcaa gaatggcttg taaatctgct
     3421 tttttggaag gctctaaaag accttctaca gtcccttttg gcagtttttc aaatgcggca
     3481 tttgttgggg catacaccgt aaatggtcct gtattgctta gtgcatctac taaatcagca
     3541 gcttttactg ctgcaactaa ggtagtatgg tcagggctgc cgcttgctac ctgaacaata
     3601 ttagggttgg agacatcatc ctttactccg gattgaccgg ttggttgtac ggtttctgaa
     3661 tctgtagttg gagatgtttg tttcggctca tttttacatg aaataataaa taaactagat
     3721 gtgaatagca aacaaattat acgggttttc ataacattaa aaatttgtca caaggtaata
     3781 acattaagca catttgaata tgattgtaat catatacttt cattttgttt tgaggattta
     3841 agctgggcta aaagaattgc cagaattcct acaactaata ttcctgaaat tattactaaa
     3901 atttgatcaa tgtgagggac aggagtgtag ctcaatgatg ctattccttg tatacctaat
     3961 gcacattcgt ttaataaaac ccctgcggca attaaaatca atgcaatttt tgtgtaaata
     4021 ttatgattta taaatttcat ggcataagca taagttagta aaaagagcga cgtaattgct
     4081 aagagaacaa gatgtaaata ggctataact ataggcctaa atccaaatgc catttcacta
     4141 attgatggta taacagaaaa taattgaaga aataatttga ccgttgttgc acaaaatata
     4201 gttataaaaa gtagtttaat tagtaagtct tttttagaaa agattgaaat aactttgtga
     4261 ttttttaaaa ataatactaa ccacaaccat ccacaaattt gacaaatagc agctaacata
     4321 atggtgatat aaactaatat aggaagattt agccataata ctgaaagccc aaatgccgga
     4381 atgcaactaa ttgcaaaata attgaaaata gctgagggaa tttctgcctg cggtaatagt
     4441 ttttttatgt aagtaaaaaa tagccccata caggcaaaga aaaaccatcc attatattga
     4501 aaatgaagat accaataaat ggaggctagg tatctttcct gttcaatttg gtgtttagcc
     4561 atcattaata cgagcgatgc agttcctgct gctgatataa tttgcatgca attcgcaaaa
     4621 atgaaccaca ttctttgggg tatagtcttg ttttgtaata aatcacttaa cgtaattatt
     4681 gcataagcaa ttgaagtaaa taaggagatg gatgatatga taatagtgaa tgtgttgtag
     4741 ccattgattg taaaccctat caacatcccg aaggcacaaa taatattgag taaaatacaa
     4801 atttgataat accgtaaacg tttaggtgaa atgttttgtg caatagcaag tgttataaat
     4861 aacatgagta tatgggatac ccatcctgaa aatgcaaaat gagaatgcgc gtgtaataag
     4921 tttttctggt caaaaaaggg aaatgaatac ccaatcttgt agcgcataat aaagcctaca
     4981 aatgcaacga ttgaaaaatt aaacaaacca agcttaaccc aaaatttatt gttaattgtg
     5041 tacatttaaa aaaataactt gcggtcttta attataatca tgttttttga tttcattact
     5101 gaaagagttc ttattactgt ctcaactctc aatccggtgt agttggcaat ttcctgtctg
     5161 gtaaaaggaa tttggatatg attttcgtgc tcttttaatt ttcttttata gtcacataaa
     5221 aatgccatta tacgttcttc aggtgtgtga ttaataacag atttggctgt tatcgctttt
     5281 gaataaacct tttttgcaag aatttttaaa aaggtaaatt gtagctctgt gttttcacga
     5341 agtattttaa aaaaatattc ttttgaaatt cttaaaacta ccaaatcctc atttgcaatt
     5401 gcggaagccg gatacttttc atttaaaaaa agtggaggct cgccaaaact ttcaccatta
     5461 ctaaacattc cttgtatata ttccttgcca ctatcattca tattagacat cctcaactcc
     5521 ccactttcaa tctgataata aaataaagct tcatcccctt cataaaaaat atattcaccc
     5581 tttttgtatt ttttgtaggt tgctccccat gagattaata aattggtatc aattgatgac
     5641 atataaaaac aaatttacaa cagaatagat taaccctatg gataccgcct tactattcaa
     5701 aaatttaagt tatttatgcc cgcaaaattg gctgaagatg tgaattaatc atatgattgt
     5761 aatcataata ataatttgct attggttaag ccttgtgata aaccctcttg attttagaca
     5821 aataaatcca tgtttaacta ctttttgcaa cgcatacagg tggaagtgcc ttttcacaag
     5881 ccaggtgtgc gttctgtttt ctcccttcaa tttcttaatt ttgcccaaaa taaccaattg
     5941 ttatggctga tattcatcaa ttaaaacgca tttgttcaca ggtaagaaga gatatagttc
     6001 gtgaagttta ccaatgccaa agcggtcacc cgggcggatc attgggttgc accgaatttt
     6061 ttgttgcatt gtatttcgat gtgatgaatc ataacaacaa ctttaacatg aatggggaag
     6121 gtgaagattt atttttcctg agtaatggac atatttctcc ggtttggtat agtgtgttag
     6181 caagaagcgg ttatttccca cttgaagaat taactacttt ccgcaagctg gattctcgtt
     6241 tgcagggtca ccctacaact gctgagcatt taccgggtat acgtatagca agcggttcgc
     6301 taggacaagg aatgagtgta ggaattggtg cagcacaagc gaaaaaatta aatggcgata
     6361 cacatttggt gtatacatta catggtgatg gcgaattaca agaaggccaa atatgggaag
     6421 cagcaatgta tgcagcgcat gttggcgtag ataatattat ttgcacagtt gactggaacg
     6481 gtcagcaaat tgacgggcca actaaaaaag taatggattt aggtgacctc aaagcgaaat
     6541 tcgatgcatt tggttgggat actcttgtgt gtaacgatgg aaatgatatg gaacagttac
     6601 tacttgcctt aaacacagca aaatctaaaa cagggaaagg taaaccggta atgattttga
     6661 tgaagaccgc tatgggcaaa ggagttgact ttatggaagg cagtcacgaa tggcacggaa
     6721 ttgctccaaa caaagaccaa ttggaaaaag caatggcgca attggaagaa actttaggcg
     6781 actattaatt ctcacttacc gtattgaata tttcgtaaca aattgttgcg gaaataaggc
     6841 tttagctgta acttttcggc atgaaaaaag ctaaacaatc aggtcgtttg gtgcattcag
     6901 caaaactgaa gaattattct gcactatcag catcttttat agctttagca gcacctgttt
     6961 taagtcaggt ggtatatact gatgtaaatc ctgacatcaa tgtgttcctc gatgaaatta
     7021 cgctcgactt agataataac ggaacaaatg attttaaact tgtttttgtt agtgaaacct
     7081 tcgccacttt tgctcctgca gaattaaaat tgcgcataaa tccgctcggt gaaaataaaa
     7141 ttgcttattc atttcaaaca attcctgtca cctattcagg aactccttgg tacacttata
     7201 attcaggagc aacacaaata gtattttcta atgtaccatt attaaatgaa agtgcagtaa
     7261 ttgatgaggc tatggatttt agtttggaca aggcagcact ttttgaaaaa ttatattttt
     7321 acatttatag cagttacgat ttacaggcat tcgaaggcgg atggtggaat ggaacagaaa
     7381 atcattttgc cgcatttcag ttagacattg atggcactta tcattttggt tggatgcgat
     7441 taaatgtaag tgttgaaacc ggaattactt tgttggatta tgcatttgaa tcattacctg
     7501 attcgccaat tacaactgcc attaataatt atagtatttc ctggataaat gttgcagatg
     7561 ccggaataac agcatctcct gaagatttga cttttagttt taatgctgca ggtgatgagg
     7621 aagatataga tgcctatcgg gtaatttgtg caaaagcagg aacatcaatt tcagtagccg
     7681 aagcaaatgg tttacccgcc gaccgttatg tgtcaattat tcctgatggt agtgcaagtt
     7741 ataatggcaa ttttggaaca attatagtag atagtgatgg cgatatcatt accacaaatc
     7801 aggaatatca gttatgggtt ttgcacgaat taaatgcagc taccggttat ttaaatacat
     7861 tatcaattcc ttctaacaca atacaattgg tagatacagt tgctattgtt accgacctct
     7921 caattacgga tattgataac aatggaaatg ctgcggatat tagaattgat tttgatgcac
     7981 ctgcagtaga acaaggaatt gttgcatata gaatttatat aacacaaaaa gatgcagctg
     8041 atatttttac cttatctgat gcattggttt taggtgcaga cctttatatt gaagttttac
     8101 cgggtgatgt atcctattcc ataattccaa ctccttcatt attaaatact gaaggtaata
     8161 tcattgaacc ggataaatac aaagcatttg ttgttagtat acctgatggc actaccgttg
     8221 aacatgcagc aatgactttg gcttcaaatg tgatttcact tgaacaacct acatctgtag
     8281 ttgaaaatat tttattggaa gatattgcag atgaaggcaa tggtgcagat attaaggtta
     8341 cctattttat tccttcgtac gaacaaacaa tagaatccta tcatatattt tttgtcgatt
     8401 ttgcaactgc atttgatttt gatttgagta gtgcccttgc ttcaacaaat tatatcgaaa
     8461 ttacaccaac cggcagcgat attacagtga caggtgatgc cacaacaaaa gacagtaatg
     8521 gtaatttaat tacttggggt gtgccgtatt atgcttatgt attagcactt gcaagtgatt
     8581 atggtattga tgatactttg tccttgccta gtaatcaggt gattttaaat tatccggtca
     8641 taataggtat acaggatgca gcacaacaaa tacctcaaat ttttgctcac ggcaaccaag
     8701 tagttattca attacccaat gacaatcctg ctaccgtaaa catttataat acacttggtc
     8761 aaaatatatt taatcgagtg attacatctt cagaaaaaat cactgtagac ttacctgaag
     8821 ggaactattt tgttagcata actcaacttg aatcaatcta taccagacag gtatttgtga
     8881 ctgaataaat tacctttctc tgagcccgga atatttggct gaagcaaaca tctacaaaaa
     8941 actatacacc tcataacgag gctgttaact ctaattttag tgcaaatcct taaaaacggc
     9001 agcatttttg tttatttgca ttggttcaaa ctatgaaaag cccaataaaa ccttaaaaat
     9061 catcgtttta taaggattac ggggtaagcc aagatgagaa acatcattgt tgtatgctta
     9121 ttgtttttag gattttccct ttctgcccaa acaactacgg ccggaagtgc gaagcgcatc
     9181 ttaattttat tagatggcag tggtagtatg ttagacccct ggaaagggac aaataaatgg
     9241 gaagttgcca aaaaattagt cgtaaaaacc atagacagta ttcaaaaaga agacccggag
     9301 gttgaaattg gtattcgggt atttggccat caaagtccga gagcggcaca tgattgtaaa
     9361 gactccaagc tggaagtttc catttctaaa aattcaggct ataatgttaa aaatgcctta
     9421 aataatattg tccctaaagg atatacacca atagcctact cgttattttt atcggcgggt
     9481 gattttatct caacagaagg aaccaactcg attattttga tcacagatgg tatcgaaaat
     9541 tgcgaaggtg acccatgcgc aagttctcag gcactgaggg ataaaaaaat tactttaaaa
     9601 ccttttgtaa taggtttggg tttggcagaa accgataaaa aacaatttga ttgtattggg
     9661 agttattacg atgcagggga tgaaaaatca ttttcaaatg ccatgagtat tgttgtatca
     9721 caggcattaa atattaccac tactcaaatt aatttattgg atgcttttgg attgcctgtc
     9781 gaaaaaaata ttgaaattac cttatatgac catgcaacag gtgaagtgag atataattac
     9841 gtacatactc cagattccag aaatcaacct gatactttat tccttaatcc aattgggaaa
     9901 tatgatattg ttgtgcatac atttcctatt gttacactta atgatgtgga gctaacacca
     9961 ggtaaacata atattattgg tattgatgtg cctttgggta atttaattat cagtgaagga
    10021 caaagtacat ctttcagccc taaacaatgt gtagtgcgcg ataataaaac aggcgaaatt
    10081 gtatatgccc aaaattttaa taccaaagaa agatatattg caggtgttta tgatatcgat
    10141 attttaactg ttccgaggtt acattatgaa gactttgtaa ttaaaggagg ggttgataat
    10201 aaaattgata ttcctttacc aggcacttta aatatttcaa ctacagaaaa taaagtgtat
    10261 agtatttatc tggtgaaaga aggaaaatta gaaaaagtgt acgaaagtaa tttaaatgcc
    10321 aataatgaaa tggtgcaatt gttaccgggt gattataaaa tatttagtcg ttccaacatt
    10381 aaaaaatatt cggctaacac aaaagatatt gcggtcacaa ttaaaagtgc taaaacaaca
    10441 ataattaaaa tttgagtaaa caacaaacat atcgtaacct aattctttta cttgtcattt
    10501 gttttagcgc acagatgaat gtgattgcgc aaacaacgca agtcacaagg attttgtttg
    10561 ttgttgacgc tagccgtagt atgtcgcaaa cgtgggacag gagcgccaaa atggcggctg
    10621 cgcgagcagt ggtgaatggt attgccgata gtttaaatga taatccgaat attcaaatgg
    10681 ccatgcgggt ttatggtcat caaagtccgc aacctttaaa cgattgtgaa gattcgaaat
    10741 tggaaattgg atttcgaaca aaaaatggat tgagtattaa aaacagatta gatgatattc
    10801 gtccgaatgg tgtaacacca attgcctact ctatggagca atcgctcaca gattttggtc
    10861 ctgatgcaaa aaattatcgt aacatcctca ttttagtgag cgacggtttc gaaagttgtg
    10921 gtaaaaaccc ttgtgaggta gttcagcgtt tgcgtaatat gggtgttata acaaaatcgt
    10981 atgtaattgg tattggaatt gatgcaactg aatttacaca gtttagttgt atgggggaat
    11041 ttatgaatat cgaatcggag caaaaaaccg aacaggtaat tgatgctact atcgctaaaa
    11101 tatttaactc agtttatgta aaagtagatt tgttagatac ctacaatcaa ccggaagaaa
    11161 ctgatgtgct gatgactttt tatgatgcca ccagtgatgt acagaaattt aattattacc
    11221 atacaattaa cccaaaagga aatcctgata caattacatt agacccggct ttgaattatg
    11281 acatgcaggt gcataccaca ccggaattaa tgaaaaaaga cattgaatta acacctggga
    11341 aaattaatac catcacccaa ccggcaccac agggatattt agtagttgat gtaagaaatg
    11401 aaaaatttgt ggccacatta aattgtatca tcaaaaaaga taataaaata gttacctggg
    11461 agcaaacaaa taaacctcgc aaattattag tcggaaacta tgatgttgaa atattaacac
    11521 tgcccattat taccgtaaaa aatgtgaaag tagagcagga taaaactacc acattggaaa
    11581 tagcggcacc tggatttgcc accatttcga aaactgaatc tgtttccggt ggaatttacg
    11641 aataccgaga aaataaactg gttgagattt atgaattaaa tgaatccagc ctaaaggaaa
    11701 cgctaacaat acaacccggt aaatacaaaa ttatttaccg ctacctctcc aaaaaggata
    11761 tggatgccac taaagaagtt gattttgaga taacttcggg ggcatcattt actataagac
    11821 tataatcttt gccttatctt cgcagcagat ttcggaatac ccgttatcgg aaacctaaaa
    11881 aatcgctgtt ttgatcaacg tagattacac caaccaaaaa gatacccgct caggatttgg
    11941 cgttggtatt ttggaagccg gaaaagctaa ccctcaagta gtagccttat gcgcagacct
    12001 tatcgggtca cttaagttag atgcgtttat taaagaattc cctgatcgtt ttttccagtg
    12061 tggtattgca gaagccaata tgaccggtat tgcagctggt ttaaccattg gcggtaaaat
    12121 accgtttgcc tgcacctttg ccaacttcgc taccggtagg gtatatgacc agattcgtca
    12181 gtcgattgct tattccaaca aaaacgtaaa aattgccgct tctcatgccg gggttacctt
    12241 aggtgaagat ggcgcaactc accaaatttt ggaagattta ggtttaatgc aaatgcttcc
    12301 caattttgta gtgataaacc cttgcgatta caatcaaacc aaagcagcaa ccattgccgc
    12361 tgccgaatac gatggccctg tttacctgcg ttttggccgc ccgaaatggc ctatttttta
    12421 tcctgaacag gaaaaatttg aaatcggtaa agcctggaaa attcaggatg gtaaagatgt
    12481 ttccatattt gcaaccggcc acttagtgct aaaagcaatt gaagcaacca aaattttgga
    12541 agaaaagggt atcagcgtag atttaattaa tattcatacc attaaacctc tcgatattcc
    12601 tgcagtcata gcatctgtaa gcaaaacaaa at
//
LOCUS       DAORKW010000041        12380 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_83_length_12380_cov_5.082759, whole genome shotgun sequence.
ACCESSION   DAORKW010000041 DAORKW010000000
VERSION     DAORKW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12380
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83_length_12380_cov_5.082759"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PLB46_03160"
     CDS             complement(<1..1166)
                     /locus_tag="PLB46_03160"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRF75543.1"
                     /translation="MNSQIAIHWFRRDLRLEDNTALYQALTNNEQVLPIFIFDTEILE
                     TLEDKSDARVQFIFDTLMAIRKKLRASGNDIYIAYGSPLDVFKTLIRQYAISTVYTNH
                     DFEPYAIQRDNAVSQFLQEHNIAFKTFKDQVIFEKDDVLKDDGKPYTIYTPYSKKWKL
                     KLTADDLAPKPSEKYLRYLFQTPPLPEISLQQLGFLPTKIFIPGNQLNLEIVKNYHNT
                     RDLPAVNGTSRMSVHLRFGTVSIRHLARLTQQVNEKYFNELIWREFYQMILAHFPHVV
                     TKSFKPEYDRIVWRNNESEFESWCQGKTGFPIVDAGMRELNATGHMHNRVRMIVSSFL
                     TKDLLIDWRWGEAYFAKKLLDFDLASNNGGWQWAAGCGVDAAPYFRIFNPQSQTL"
     gene            complement(1203..2045)
                     /locus_tag="PLB46_03165"
     CDS             complement(1203..2045)
                     /locus_tag="PLB46_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRF75544.1"
                     /translation="MIFYNEAFMEAGAFAVTASNRAFKYGDGVFESALVINKKVPLLN
                     YNLNRLKKSLALLGIEEPSNWNLSYFTSIITKLCEQSNIENGRCKITVWRSGEGLYLP
                     VANTADCLIEIFPHETNPLIYKGAPLVLGVYDDYPKMIHPLSACKTSNAIPYILAANY
                     AKQNGFDDVLLLNTNDGIADSISSNLFIVKNNTCYTTSVMNGGVEGTMQAYILANAVS
                     LGIKVDRIPMNINDVLSANEVMLTNAIFGIKPVTQFQDVVFKNNFTIELLNKLKWLLE
                     QDAK"
     gene            complement(2075..2728)
                     /locus_tag="PLB46_03170"
     CDS             complement(2075..2728)
                     /locus_tag="PLB46_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HRF75545.1"
                     /translation="MLDKNQIAKRIARELKDGYYVNLGIGIPTLVANYIPEGISVTLQ
                     SENGLLGMGPFPTEDKVDPDLINAGKQTITTLPGSAFFDSALSFGMIRAGKVDLTVLG
                     AMEVADNGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHVNKAGESKLLKKCSLP
                     ITGLGVVKKVVTELGVFDVTADGFKLLERAPGVTVEEIIAKTEGRLVVEGEIPEMII"
     gene            complement(2759..3451)
                     /locus_tag="PLB46_03175"
     CDS             complement(2759..3451)
                     /locus_tag="PLB46_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HRF75546.1"
                     /translation="MNKVVNNAEAAIQDIHDGATIMLGGFGLCGIPENCIAALVKKGT
                     KNLTCISNNAGVDDFGIGLMLQQRQVKKMISSYVGENAEFERQLLSGELEVELIPQGT
                     LATRCMAAGYGMPAIFTPAGVGTEVAEGKETRVFNGKTYLLEYAFDADYAIVKAWKGD
                     THGNLIYKSTARNFNPLMAMGGKITIAEVEELVPAGELDPNAIHTPGIYVHRIFQGSN
                     YEKRIEQKTITK"
     gene            complement(3432..3842)
                     /locus_tag="PLB46_03180"
     CDS             complement(3432..3842)
                     /locus_tag="PLB46_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HRF75547.1"
                     /translation="MGRITVISLVFLVFAAACKPHSAFEVDAEMINNVPDSVIAVEPM
                     IKIMADVHLAEAWVQENKTDTIPQDIRLKAYYQKIFDLHGITSTAYQNSYNYYSAHPA
                     LMSHIYTKVVEQLNVLESESAKVKKKIPNEQSSK"
     gene            3880..4539
                     /locus_tag="PLB46_03185"
     CDS             3880..4539
                     /locus_tag="PLB46_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRF75548.1"
                     /translation="MMSNFEDIKLYLDEKNVTLVAVSKTKPTTAILALYDKGQRDFGE
                     NKVQELREKYEALPKDIKWHLIGHLQTNKVKYIAEWVYMIHSVDSLPLLEEINKQAIK
                     HNRIIPCLLQTYIAKEETKFGMDEAELHELLQSSTFAGLKNISIYGLMGMATNTNDEQ
                     IIRAEFLHLKAIFNTLKQTYFNAETQFSMLSMGMSSDYKIAVECGSSMVRIGSLLFGE
                     R"
     gene            4628..5665
                     /locus_tag="PLB46_03190"
     CDS             4628..5665
                     /locus_tag="PLB46_03190"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HRF75549.1"
                     /translation="MNIKLIFPSLLLILGSCAYMPVKRAFTEEKLPTSPDYSQETYWS
                     ALPSKKDSADHIPKNSVFEIVDNQNNATADVFFIYPTHFFSRKEWNADVNDTKLNNHI
                     AERSIYHQASVFNGSCKVYAPYYRQATFNAYFSLDNPDAYKAFDLAYQDVKNAFQYYL
                     DHYNNGRPIIIAGHSQGTTHAKWLLRDFFEGKPLQKQLVCAYLIGMPVYDYEFTTIAP
                     CDSANQTGCFVSWRSYLSGSEPDKKMQIPTPEKVIVHNPLTFTRATGIAKAELNEGGL
                     GRDGNSIYPQVCTAEIHNDIVWVSRPEIPYNKFVPKNLHPADYNLYWMVIRKNVAERI
                     AVWENNNTSQK"
     gene            complement(5666..6601)
                     /locus_tag="PLB46_03195"
     CDS             complement(5666..6601)
                     /locus_tag="PLB46_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRF75550.1"
                     /translation="MKAYTMNKKWFVIINPVSGGKYAPKLWNKMLPHMETTGISFQVA
                     FTEYIDHAGIIVKQAVDDGFQHFMIIGGDGTANDVINGIFNSTGQPENFVVAMIPAGT
                     GNDWVRTIGEYKNIMEIPEKLQSGNTFLQDVGLVTFQKENATRTRYFINIAGLGFEGQ
                     VAKKLYEKDGLLKGTKLQYQLAILQSLFVYKHTRLTLTVDGVTQSQTGLSIAAGICKY
                     NGGGLKQLPNAIIDDGLLDMTVIGSMTKLQMVMNLPKLRNGSHVKLKSVNTFRGKTIT
                     INSEPPIYLDADGEFLGTTPVTISLIPKAIQVLQF"
     gene            6592..8001
                     /locus_tag="PLB46_03200"
     CDS             6592..8001
                     /locus_tag="PLB46_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HRF75551.1"
                     /translation="MLSNSEFRQQAHKAVDWIADYLDNIEKFPVKSQILPGSIKAQLP
                     ENAPGKGENMDIIFDDFASIIPPGITHWQHPGFMALFPSNHSYESVLAEMLAAGLGIN
                     AMVWETSPAATELEERMMEWIGNNFGIPPGWKGVIHDTASTSTLTAIISAREKYSQFK
                     INQHGYNNFNALTLYCSAQTHYSIEKGAKAAGIGLDNVIKIATDEHYAMQAAALEIQI
                     QNDLAVGKQPFMVVAALGTTSSLACDPLAEISAICKKYNLWLHIDAAYAGAAFVLEEY
                     HHYLKGIIDADSYVFNAHKWLFTNFDCSLYFVKDAQLLINTFSSNPDYLKRTMDEVTN
                     FKDWGLPLGRRFRALKLWFMLRSFGIDGIKVQLRKHIELAQHFHDTLLSESNWELMAP
                     FQMNVLCIRFHPAHIQDNAELNKINENIIKSINATGKFYLSGTRLNGNFVIRVVIGQT
                     HVEKRHVEELVMLLNQVTP"
     gene            complement(8003..10504)
                     /locus_tag="PLB46_03205"
     CDS             complement(8003..10504)
                     /locus_tag="PLB46_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75552.1"
                     /translation="MKYFLLLTSIAILGITYQITQKTDSGTVYYESETKSLEALAWIA
                     QIHDYPNQSSPDGRFYESFLYFKEHYLNSNKARLTDEGEWENIGPNNVGGRTISIAIH
                     PIDTNIIYMGSASGGLWKTTTGGIGVNAWQFVETGYPILGVSAIAINPENPDEIYIGT
                     GETYAYGKATNGLIDRTERGTFGMGILKSHDGGANWEINLNWTYQLNHCVWDIVFDPF
                     NTDQLYAATTEGLYKTTNGGLYWSQRLDEFMVMDIEVNKLAPHVLFAGVGNLNSANKG
                     LYRSEDYGETWELMDNGLPPYTHDGRITVTSNINNASEVISVIANAFSTVGIYKSTNG
                     GDSWFELDDADVASYQGWYAKGALIHHENPNLILLGGVELFKSINGGNVFVQKTTYTG
                     PDSEMHPDIHDIVANPLDPDKVYILTDGGLFRSNDFGETFFSCNDGYVTSQFYIGSIS
                     AQTNDKGLGGLQDNFTQRYDGSKYWDAIIGGDGSFNAIRPDDDEIQFASYQYGVVYKS
                     NDGGNNFDEFVFSPTGDAAFVTPFVLCPSDPESMYIGDTKLHYSNNSGGSFSLTSPAL
                     IDSGNVVLSIGVSGLSIDTVFISTAGLSGRADMFISTNGGDSYTAITNGLPDRYYRDI
                     AVNPNNANELYITLSGYGTGHVFRSMDAGSNWVDVSESLPDMPFHSVAIDPSNDSIIY
                     VGCDFGVFVSFDKGLSWENFGTGLPEAVMVFDLVFSNSDNMVLAFTHGNGVYRIPQAS
                     KPTQVSINTFDDESITLTPNPVADYLTVNISDGRYTHCAAKILNSRGEVVFQKNDFTL
                     ENWMRIYCNQLASGIYFLVLENNQFKHIEKFVKTP"
     gene            complement(10711..12105)
                     /locus_tag="PLB46_03210"
     CDS             complement(10711..12105)
                     /locus_tag="PLB46_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HRF75553.1"
                     /translation="MLNDTFSRKVFTKQPEITFRDRKLYPYTKGYWIAQPILIGVPYR
                     YYYSRFQVNGWENVPTDKPVIFAISHRNAFMDSLAFVNTKSTQVWQLARGDAFNNKLL
                     GNLFYFFHMLPLWRERDGVDTREMNQPTFNACADLLANNAMIGIYPEGNCINEEHIRP
                     LKKGICRIAFLAEERYNFELDVHIIPVGINYTGAEKFKKWQLVNFGQPILLKKYSEQY
                     KTNPALAINHLKDEIERGMKKVNTHVEHGPLHHDIVELAEFHARESIIISGKPYEPIT
                     RFDAIKSITPILENNNKNNHEAFKNIVADYHAYKTQRDTLGFRENTFDKARQSNLSMI
                     FMALYFIFLLPVFLFGLIINYIPYRIPQIYAEKKLKQQIFWSSAKYVLGFMVFPIYYL
                     ILMLIVWSMLGSLLSALIFLVAFPISGNIAFYYWYDFKKWWSVLRFKRMSAEIKAQLT
                     FQREKLYNTLNSLS"
     gene            complement(12131..>12380)
                     /locus_tag="PLB46_03215"
     CDS             complement(12131..>12380)
                     /locus_tag="PLB46_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75554.1"
                     /translation="FAMLSGYGVGGLFKLDFQDKKCLAIETGIHNAALGLIIIFTFFN
                     GMGGMAVVAGWWGIWDLVTGFVVATIWARMAAKQAAKG"
BASE COUNT         3845 a         2440 c         2162 g         3933 t
ORIGIN      
        1 agggtttgtg attgcggatt gaagatgcgg aaataaggag cagcatctac accacaacct
       61 gcagcccatt gccatccgcc gttgttgctg gctaaatcga aatccaataa tttttttgca
      121 aaataagctt caccccaacg ccagtcgatt agtaaatctt tggtgaggaa actggagaca
      181 atcatacgca cacgattatg catgtgccct gttgcattga gttcacgcat accggcatct
      241 acaatgggga atccggtttt tccttgacac cagctttcaa attcggactc attatttctc
      301 caaacaatcc tatcatattc cggtttaaac gatttagtta ccacatgcgg aaaatgcgcc
      361 aaaatcattt gataaaattc gcgccagatt aattcattaa aatatttttc atttacttgt
      421 tgggttaatc gagccaagtg tctaatactc accgtcccga aacgcaaatg cacactcatg
      481 cgcgatgttc cattcacagc aggtaaatcg cgggtgttat gataattttt tacaatttcg
      541 agattgagtt gattgcctgg aataaatatt tttgtcggta aaaatccaag ttgctgtaaa
      601 ctgatttccg gtaatggagg tgtttgaaat aaatatctca ggtatttttc cgatggcttt
      661 ggtgcaaggt catctgcagt taatttcaat ttccactttt tgctgtaagg tgtataaatg
      721 gtatagggtt taccatcatc tttcagcaca tcatcttttt caaaaatgac ctggtcctta
      781 aatgttttaa acgcaatatt atgttcctgt aaaaattgac taacggcatt atcacgctga
      841 atggcatagg gttcaaaatc gtgatttgta taaaccgttg agatggcgta ttgccggatt
      901 aaggttttaa acacatccaa gggtgaacca taggcaatat atatatcgtt accggatgca
      961 cgcaattttt ttctgatagc catcagtgta tcaaaaataa attgcactct tgcatccgat
     1021 ttatcttcta gggtttccaa aatttcggtg tcgaaaataa atattggcaa cacttgttcg
     1081 ttattggtaa gtgcctgata taaggcagta ttgtcttcca aacgcaggtc gcgcctaaac
     1141 caatgtatcg caatttgaga gttcaaggta gtaattgaat tagggtgtaa actaaactaa
     1201 ggttatttag catcttgctc taataaccat ttcagcttgt ttaacaattc aattgtaaaa
     1261 ttgtttttga atacaacatc ctgaaactga gtaaccggtt ttatgccgaa gatggcattg
     1321 gtcaacatca cttcatttgc gctcagcaca tcgtttatgt tcataggaat acggtcaact
     1381 tttataccca gactgactgc attagctaaa atataagctt gcattgtacc ttctacccct
     1441 ccgttcataa ctgaggtggt atagcaagta ttatttttta cgataaacag attggaactt
     1501 atgctatcgg caattccgtc gtttgtattg agcaacaaaa catcatcaaa accattttgt
     1561 ttggcatagt ttgctgctaa aatgtagggt atcgcattcg atgttttaca tgccgatagt
     1621 gggtgaatca ttttaggata atcgtcatac acacctaata ctaacggagc acctttataa
     1681 ataagcggat tagtttcgtg cgggaaaatc tcaattaaac aatccgcggt atttgcaacc
     1741 ggtaaataca aaccttctcc tgaacgccaa actgtaattt tacatctgcc attttcgata
     1801 ttcgattgtt cacataattt cgttataatg gaagtgaaat aggaaagatt ccaattggat
     1861 ggttcttcta ttcccagcag ggccaaactt tttttcaggc gattaagatt gtagtttaac
     1921 agcggtactt ttttatttat caccaatgcc gattcaaata ccccatctcc gtacttaaaa
     1981 gcgcgattgc ttgctgtaac agcaaatgca ccggcttcca taaatgcttc attataaaaa
     2041 atcatatgca caatttatat gaatgaacgt tcattcatat tatcatttcc ggaatttcac
     2101 cttcaactac aaggcgtcct tctgttttgg caattatttc ttcaacggta acaccgggag
     2161 ctctttcaag tagtttaaaa ccatcggcag ttacatcaaa aacgccgagt tcagtaacta
     2221 ctttttttac tacaccgaga ccggttattg ggagcgaaca tttttttaat aatttacttt
     2281 ctccggcttt atttacatgc tgcatggcta cgataatatt tttagcgctg gcaacaaggt
     2341 ccattgcacc acccattcct tttaccattt tacccgggat tttccagtta gcgatatcac
     2401 cgttatcggc cacttccata gcgcctaata ctgtgagatc cacctttccg gcgcgaatca
     2461 ttccaaagct taaagcactg tcgaaaaaag cggaacccgg caatgtggta atagtttgtt
     2521 tacctgcgtt aattaaatcg gggtctactt tatcttcagt tggaaaaggg cccattccga
     2581 gcaatccgtt ttcactttgc agggtaacag aaataccttc ggggatataa ttagcgacta
     2641 atgttggaat accgatgcca agatttacat aatatccatc ctttaattcg cgtgcaattc
     2701 tttttgcgat ttgattttta tctaacataa tactttttga gtaataagtg atgcaatatt
     2761 atttagtgat ggttttttgt tcaatacgtt tttcataatt acttccctga aaaatgcgat
     2821 gaacataaat gcccggtgtg tgaattgcat tagggtccaa ttcaccggca ggaactaatt
     2881 cttccacttc tgcaatcgta atttttcctc ccattgccat caatgggttg aaattgcgtg
     2941 cagtagattt ataaattaaa ttgccatgcg tatcaccttt ccaagctttt acaattgcgt
     3001 agtcagcatc aaatgcgtat tcaagcaaat aagtttttcc attaaatacc cttgtttctt
     3061 ttccttcggc aacttcagtt cccacaccgg ccggagtgaa gatagccggc atgccatatc
     3121 ctgcagccat gcaacgcgta gctagagtac cttgcggaat taactccact tctaattctc
     3181 cgcttaataa ttggcgctcg aattctgcat tttccccaac ataactactt atcatttttt
     3241 tcacctgccg ttgttggagc attaatccga ttccgaaatc atcaacacct gcgttattgg
     3301 aaatgcaggt taaatttttt gttccttttt ttactaaagc ggcgatacaa ttttccggta
     3361 ttccgcacaa tccaaaacca ccgagcatga tagttgcgcc atcatgaata tcctgaatgg
     3421 cagcttcagc attatttact actttgttca tttggtattt tttttttcac ttttgcactt
     3481 tcactttcca aaacattgag ttgctcaacc actttggtgt aaatatggct cattaaagcc
     3541 gggtgcgctg aataataatt ataactgttt tgataggcag ttgaggtaat gccatgcaaa
     3601 tcgaatattt tctgataata cgcttttaac cgaatatcct gagggatggt gtcagtctta
     3661 ttttcctgca cccaggcttc agctaaatgc acatcagcca taattttaat catgggttct
     3721 acagcgatta cggagtccgg cacattgtta atcatttcgg catcaacttc aaacgcacta
     3781 tgtggtttac aagctgcagc aaaaacgaga aacactagag aaatcactgt aatccgcccc
     3841 attacctcaa aaggatttaa ttttgcgcta agttagccaa tgatgagcaa ttttgaagat
     3901 attaagttat acctcgatga aaaaaatgtc accctggtgg cagtatcaaa aaccaagcct
     3961 accactgcca tattagcgtt atatgacaag ggtcagcgcg attttggtga aaacaaggtt
     4021 caggagttgc gcgaaaaata tgaggccttg ccaaaagaca tcaaatggca tctcataggc
     4081 catttgcaaa ctaataaagt aaaatatata gcagaatggg tttatatgat tcattctgtt
     4141 gatagtttac cattgttgga agaaatcaac aaacaggcaa ttaaacacaa tcgaattatt
     4201 ccttgtttgc ttcaaaccta tattgcaaag gaagaaacaa aattcggaat ggatgaggca
     4261 gagttacacg agttattaca aagttccact tttgccggtt taaaaaacat ttcgatatat
     4321 ggcttaatgg gcatggcgac gaatacaaat gatgaacaaa taattcgcgc agaatttttg
     4381 catttaaaag caatattcaa cactttaaaa caaacttatt ttaacgcgga aacacaattt
     4441 tctatgttat cgatgggcat gagcagcgat tataaaattg ctgttgaatg cggtagttcg
     4501 atggtgagga ttgggagttt gttattcggc gaacggtaag aaaaaacaga cctactaggt
     4561 ttaaaccttc caggtttaaa cctggaaggt ttttaactaa aaaaaatcaa caattacaaa
     4621 caaaaaaatg aacatcaaac ttatcttccc atcacttctg ttaatattag gctcttgcgc
     4681 atacatgccg gttaaacgcg cctttacgga agaaaagtta ccaacaagtc cggattatag
     4741 tcaggaaacc tattggagtg ctttgccatc taaaaaagat tctgctgatc acattcccaa
     4801 aaattcagta tttgaaattg ttgacaatca aaataatgcc actgcagatg tattttttat
     4861 ttatcctaca catttttttt ctcgcaaaga gtggaacgca gatgtaaatg atactaaatt
     4921 aaataatcac attgccgaac gttctattta tcatcaggcc agtgtattta atggcagttg
     4981 taaagtttat gccccctatt atcgccaggc tacatttaat gcctattttt ctttggacaa
     5041 tcccgatgcc tataaagcat ttgatttggc ttatcaggat gtgaaaaatg cctttcaata
     5101 ttatttagac cactataata acggacgacc aattataatt gccggtcata gtcagggaac
     5161 tacacatgcc aaatggttgc tgcgtgattt ttttgaagga aagccattac aaaaacaact
     5221 tgtttgtgcc tatttaattg gtatgcctgt ttatgattac gaatttacaa ctatagcacc
     5281 ttgcgacagc gccaatcaaa ccggatgttt tgttagttgg agaagttatt tatcaggaag
     5341 cgagccggat aaaaaaatgc aaattccaac acctgaaaaa gtaattgtcc ataatcctct
     5401 cacctttacc agagcgaccg gaattgcaaa agcggaatta aatgaaggcg gtcttggcag
     5461 agatggcaac agcatttacc ctcaggtttg tactgcagaa attcataacg atatagtttg
     5521 ggtgagcaga ccggaaattc catataataa atttgttcct aaaaatctac accctgccga
     5581 ttataatttg tattggatgg tcataagaaa aaatgttgcg gaacgtattg cagtctggga
     5641 aaataataat acctcacaaa agtgattaaa attgcaatac ctgaattgct ttaggaatta
     5701 gcgaaatggt tacaggtgtt gtccctaaaa attcaccatc ggcgtccaaa taaataggtg
     5761 gctcactatt gatagtaatt gttttaccac gaaatgtatt taccgatttc aatttaacat
     5821 gtgacccatt tcttaattta ggtaaattca ttaccatttg caattttgtc atgctgccaa
     5881 taacggtcat gtccaataaa ccatcgtcga ttattgcatt tggtaattgt tttaatcctc
     5941 caccattata tttacaaatc cctgcagcaa ttgataagcc tgtttgcgat tgtgttacgc
     6001 catctacagt taatgttaat cgggtatgtt tgtatacaaa taatgattgt aaaatggcca
     6061 attgatattg caatttcgta ccttttaaca aaccatcctt ttcgtataat ttttttgcca
     6121 cctgaccttc aaaccctaac ccggcaatat tgataaaata tcttgttctt gttgcattct
     6181 ctttttggaa agttaccaaa cctacatcct gtaaaaatgt atttccgctt tgcagttttt
     6241 caggtatttc cattatgttt ttatattctc cgattgttct tacccaatcg ttgccggttc
     6301 ctgccggtat catggctaca acaaaatttt cgggctgacc ggttgaatta aatatgccat
     6361 tgatcacatc atttgctgtg ccatcaccac caataatcat aaaatgttga aacccatcat
     6421 caactgcttg ttttacgatt attcctgcat ggtcaatata ttcggtaaaa gcaacctgaa
     6481 acgaaatgcc ggttgtttcc atgtgcggta acattttgtt ccataactta ggcgcatatt
     6541 ttccccctga aaccggatta attattacaa accatttttt attcatagtg tatgctttca
     6601 aattcagaat ttcgtcaaca agcgcataag gcagtggact ggattgccga ttatctcgac
     6661 aatattgaaa aatttcctgt taaatcgcaa atattaccgg gaagtattaa agcgcaattg
     6721 cccgaaaacg caccaggcaa aggcgaaaat atggatatta tttttgatga cttcgcctct
     6781 attattccac cgggaataac gcattggcag catccgggat ttatggcatt attcccaagc
     6841 aaccacagtt acgaaagtgt attggccgaa atgttagcag cgggattggg cattaatgcc
     6901 atggtttggg aaacatcccc ggctgcaaca gaattggaag agcgaatgat ggaatggatt
     6961 ggaaacaatt ttggcattcc tcccggatgg aaaggcgtaa ttcatgatac tgcttccact
     7021 tcaacgctta ctgctattat ttcagctcga gaaaaatata gtcagtttaa aattaatcaa
     7081 catggttaca acaattttaa tgcactgaca ctttattgtt ctgcacaaac acattattcc
     7141 atcgaaaaag gtgcaaaagc tgccggaatt ggtttggata atgtaattaa aattgccacc
     7201 gatgaacatt atgctatgca ggcagcagcg cttgaaattc aaattcaaaa cgatttagct
     7261 gtcggtaaac aaccctttat ggttgttgct gcattgggca ctaccagttc attggcatgt
     7321 gaccctttag cagaaattag tgccatttgt aaaaaatata atttatggtt gcatattgat
     7381 gctgcctatg caggtgcggc ttttgtatta gaagaatatc accattattt aaaaggtata
     7441 atagatgccg atagttatgt ttttaatgcg cataaatggt tatttaccaa ttttgattgc
     7501 tctttatatt ttgtaaaaga tgctcaatta ttaattaata cattttcatc taatcccgat
     7561 tatttaaaac gtaccatgga tgaggttacc aatttcaaag actggggatt gccgttagga
     7621 cgaaggtttc gtgcgttaaa gttgtggttc atgttacgca gttttggtat tgatggtatt
     7681 aaagttcaac tgagaaaaca tattgaatta gcacaacatt ttcacgacac cttattatcc
     7741 gaaagtaatt gggaattaat ggcaccattc cagatgaatg tgttgtgtat tagatttcac
     7801 ccggctcata ttcaggataa tgctgagtta aacaaaatca atgaaaacat tattaagtcg
     7861 ataaatgcaa cgggtaaatt ttatttatcc ggcacacgat taaatggcaa ctttgtcatc
     7921 agagtagtga taggtcaaac acatgttgaa aaaagacatg tggaggaact tgtaatgtta
     7981 cttaatcagg ttacacctta atttaaggtg ttttaacaaa tttttcgata tgtttaaatt
     8041 gattattttc caataccagg aaataaatac ctgatgctaa ttgattgcaa taaatacgca
     8101 tccagttttc cagtgtgaaa tcattttttt gaaacactac ctctcccctg ctatttaaaa
     8161 ttttcgcagc gcaatgcgta tatctgccat cagaaatatt tacagttaaa taatcagcaa
     8221 cagggttcgg tgttaatgta attgattcat catcaaaagt attaatactc acctgtgtag
     8281 gtttactagc ttgaggtatt cggtaaacac cattaccatg tgtaaatgct aaaaccatat
     8341 tatctgaatt ggaaaatacc aaatcaaaaa ccataactgc ttccggtaaa ccggtgccaa
     8401 agttttccca gctaaggcct ttatcaaatg aaacaaatac accaaaatcg caaccaacat
     8461 aaatgatact gtcatttgaa gggtcaattg ccacgctatg aaaaggcatg tcaggaagcg
     8521 attcacttac atctacccaa ttacttcccg catccattga gcgaaataca tgacctgtgc
     8581 cgtaacctga taatgttata tataattcgt ttgcattgtt gggatttacc gctatgtcgc
     8641 gataataacg atcaggcaaa ccattggtaa tggcagtata actatcgcca ccatttgttg
     8701 aaataaacat atcagcacga ccggataaac ctgcggttga aataaaaaca gtatctatac
     8761 tcaaacctga aacaccgatt gacaatacca cattaccact atcgattaag gccggtgatg
     8821 ttaatgaaaa ggacccaccc gaattatttg aataatgtaa tttcgtatca ccaatataca
     8881 tactttcagg gtcagacgga cataaaacaa atggtgttac aaatgcagca tctcctgttg
     8941 gagaaaatac gaattcatca aaattatttc caccatcatt tgatttgtat accacaccat
     9001 attggtaaga tgcaaattga atttcatcgt catcaggtct gatagcgttg aacgaaccat
     9061 caccaccaat tatggcatcc caatatttgg aaccatcgta acgttgagta aaattgtcct
     9121 gcaaaccacc taaaccttta tcgttggttt gggcactaat cgatccaata taaaactgtg
     9181 aagtcacata tccatcgtta caactaaaaa aggtttcccc aaaatcgttg gagcgaaata
     9241 aaccgccgtc tgttagaata tatactttat cagggtctag tggattggca acaatatcat
     9301 gaatatcggg atgcatttca ctgtccggtc cagtgtaggt agttttttgt acaaacacat
     9361 ttcctccgtt aatggattta aataattcaa cacctcctaa taaaatcaaa tttggatttt
     9421 cgtggtgaat taatgcccct ttggcatacc aaccctgata actggccaca tctgcatcat
     9481 ctaattcaaa ccagctatca ccaccatttg ttgatttgta aattcccaca gtgctaaatg
     9541 catttgctat aactgatatt acttctgacg cattattaat atttgatgtt acagtaattc
     9601 gaccatcatg ggtatatggc ggtaaaccat tatccatcaa ttcccatgtt tcaccataat
     9661 cttcggagcg gtaaagacct ttgttagcag agttcaaatt ccctactcct gcaaataaca
     9721 catgtggtgc caatttattc acttcaatat ccatcaccat aaattcatct agacgctggc
     9781 tccagtataa tccaccatta gttgttttat ataaaccttc tgtagttgca gcatataatt
     9841 gatcggtatt aaatggatca aaaacaatat cccaaacaca atgatttaat tggtaagtcc
     9901 aatttaaatt tatttcccag tttgctcctc catcatgcga ttttaaaatg cccattccaa
     9961 aagttcctct ttcggttcgg tctattaatc catttgttgc ttttccatat gcataggttt
    10021 caccggtgcc aatataaatt tcatcaggat tttccggatt aatggcaatt gcgcttacac
    10081 ctaaaattgg atatcctgtt tccacaaact gccaggcatt aacaccaatt cctccggtag
    10141 ttgttttcca taaacctcca ctggcacttc ccatataaat tatatttgta tcaatgggat
    10201 gaatagcaat acttatagtt cgtcctccaa cattattagg acctatattt tcccactcac
    10261 cttcatcggt taatcttgct ttattactat tgagatagtg ttccttaaaa tataaaaagg
    10321 actcataaaa tcttccatcc ggagacgact gattaggata atcatgaatt tgtgcaatcc
    10381 atgcgagtgc ctcaagcgat ttggtttcac tttcgtaata gacagtgccc gaatcggttt
    10441 tttgtgttat ttgataagta attcctaaaa tggcaattga agtgaggagt aaaaaatatt
    10501 tcatgacatg taattagcct gtgaatgaat aacaggaact aatttacaaa aattctataa
    10561 tgttgtctgc agttctctcc aaattagcct tgattttaca tttgcatgaa ttagaaggct
    10621 taaaatcgca ttagttctca atgtccttta attgcaatct agcttgcaaa tgactagcat
    10681 ttaagaccga gcagcgcaaa taaaagtgtt ttaacttagg ctatttaagg tattgtacaa
    10741 tttctccctt tggaaggtta attgtgcctt gatttctgcc gacattcgtt taaagcgaag
    10801 cactgaccac cactttttga aatcgtacca ataataaaag gctatattac cggaaattgg
    10861 aaacgctacc aggaaaataa gcgcagataa taaactgcct aacattgacc atactattaa
    10921 cattaatatc aaataataaa tagggaatac cataaaacca agcacatatt tggctgagct
    10981 ccaaaatatt tgttgtttca attttttttc ggcataaatc tgtggtatgc ggtaagggat
    11041 ataattaatg attaatccaa acaaaaacac cggcaataaa aaaataaaat acaaggccat
    11101 aaaaatcatc gacaaatttg attgtcgagc cttatcgaat gtattttctc taaatcctaa
    11161 cgtatcacgt tgcgttttat aggcatgata atctgccaca atatttttaa acgcttcgtg
    11221 attgttttta ttattgtttt ccagtattgg agttatagat ttaattgcat caaaacgtgt
    11281 tattggttcg tagggtttac cggaaataat tattgattca cgtgcatgaa attcggcaag
    11341 ttcaactata tcatgatgca gaggtccatg ctcaacatgc gtatttactt ttttcattcc
    11401 ccgctcaatt tcatccttta aatgattgat ggctaaagca ggattagttt tatattgttc
    11461 gctgtatttt ttaagtaaaa tgggctgtcc aaaatttacc aattgccatt ttttaaattt
    11521 ttcggcaccg gtataattta taccaaccgg tataatatgt acatccaatt caaaattata
    11581 gcgctcttcg gcaagaaatg ctattcggca aatacctttt ttaagcgggc gaatatgttc
    11641 ctcgttaatg cagtttcctt caggataaat accaatcatg gcattatttg ctaaaaggtc
    11701 ggcacaagca ttaaatgttg gctgattcat ttcacgggta tctacaccat cgcgttcgcg
    11761 ccataaaggg agcatatgga aaaaataaaa caagttgcct aataacttat tattaaaggc
    11821 atcgccacga gctaattgcc aaacctgtgt gctttttgta ttcacaaaag cgaggctatc
    11881 cataaacgca ttgcggtgac taatagcaaa aatgaccggt ttatcggtag gaacattttc
    11941 ccaaccgttt acctgaaagc ggctgtagta atatcggtaa ggtacaccaa taagtatagg
    12001 ttgagcaatc caataccctt tggtataggg atacaatttc ctatctctga atgtaatttc
    12061 aggctgttta gtgaaaactt tgcgtgaaaa agtgtcgttt aacataaaaa accgtgcaaa
    12121 cttatcactt ttatccttta gctgcttgtt ttgcagccat tcttgcccat attgttgcca
    12181 ccacaaagcc ggttactaag tcccaaatac cccaccagcc cgcaacaaca gccattccgc
    12241 ccattccatt aaaaaaggta aaaattatga ttaacccaag tgctgcattg tgtatacctg
    12301 tttctatagc gaggcatttt ttgtcctgaa aatcgagttt gaataatccc cccaccccat
    12361 acccagacaa cattgcaaag
//
LOCUS       DAORKW010000042        12209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_87_length_12209_cov_4.388350, whole genome shotgun sequence.
ACCESSION   DAORKW010000042 DAORKW010000000
VERSION     DAORKW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87_length_12209_cov_4.388350"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PLB46_03220"
     CDS             complement(<1..1154)
                     /locus_tag="PLB46_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF75555.1"
                     /translation="MPNTLSPQQKVYGLSKFWQEVNYNFVFYNRVDKKEWDSLYQVMI
                     MEVQATPNDYEYYKKLEQFCAYLKDGHTNVYYPQAVSEKMMTTMFGNYRLFLTNFDGK
                     AIITRVNQSKKDEIPAGSEIITVNGMPTADYIAQYVRPFIASSTDYVRENQCVYQLLK
                     GFEGQEFTITIKKPNGSTQQLTLTHARTEEQEIYPPFEDFNLLSYKSLENDIAYLQLN
                     SFENESLDSLFQAVLPEIYKAKAVIIDLRNNGGGSTYIGLNVLKYFVDDTLMYGSRSE
                     SRMHIPTYKAWGTFLTPADTLQDNPDWGMSKEEMTKSYLIANDNYYYQFDYYPDTIQL
                     TAKRMNVPTVLLTSNNTASAAEDFLIYADGYKHITTIGSPTYGSTGQPYL"
     gene            1463..2509
                     /locus_tag="PLB46_03225"
     CDS             1463..2509
                     /locus_tag="PLB46_03225"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF75556.1"
                     /translation="MYFIRVLFYVVALTIVANSIFAQNIDNTTSRLDSLLNAVEVRPF
                     NGVILIKQQGKVLYENALGFADYDKKIPNKMENEFVLLSNSKQITAVLILMAADDGLL
                     QLENPIGQYLPTLSMPWADVITIHQLLNHSSGLDAADKPLLFEPGTSFKYADINYVLL
                     GQIIESVRHQTYADIATALFNKLGLKHTYVPNAYNQQNLIIGKKMIEGELPQLIDSVI
                     ISPHKIPAAGIVSTAFDFSNWVELLHTGKILSDTIYQKLIHYNITAQHVAFGPQKIGY
                     SYGLRIDDLNEPVYYGHTGTSPDQGYTSFTLWFPKSQTSVVVLENCAYDNMDVNYYYE
                     AQIRAIMTQSNWVK"
     gene            2591..3079
                     /locus_tag="PLB46_03230"
     CDS             2591..3079
                     /locus_tag="PLB46_03230"
                     /inference="COORDINATES: protein motif:HMM:NF020961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HRF75557.1"
                     /translation="MATVPHFGDIELDNLSDYYEADYNYLGKSIAIDLNFEDITIEAS
                     DLEPTIVALNGLNKLIETAYAELTKSYQTNGPVKQYINRHMSGLNPEELATLFEAAPE
                     GLNKELKMMSQLKLRRIGFYPEDTIGLVSMDFTIGYELTDDVIAIYFNNKLQLEDIAL
                     EN"
     gene            3084..5255
                     /locus_tag="PLB46_03235"
     CDS             3084..5255
                     /locus_tag="PLB46_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75558.1"
                     /translation="MMRKTIITIFSVISFFLQLHAQSLSDSLLAHYTFSDNLLDGSEN
                     HYDILSASGIFTTDRFGQSNAAFAFDGINDSLVLPIPEMAPVSGDFAISFWYKTNSAK
                     IMNLFSSKQTPDDTTHNFEMQLNSHNQYYLEYYQQVWYQTFVYWNGSGVDSNALAEGV
                     AGNFIKDEWCHFVITREADTFKIYRNHQLYFLSIDNYYGDELGDAVDLIFSASPYRFK
                     GAVDDIRCYNRNLTQQDVDALWFENNPIQFVTPQATDAYVFGSNVLVYWEYDTAQISD
                     SILVQYRINNGDWINSIHSNLIWENYTYIDMSYEPGTAVEVRVADYTDTSIFQLTGQF
                     IVSPYDWISVNDSLPFAAKDGAGLVSFKNKMWLLGGWDPPFHEPNYTHNEVWSSIDGV
                     TWNFETLAPWPPRHCAAWLSDENYIYVIGGDPQSGCLTDVWQSADGINWVQLVDTIPG
                     YKKRNMVNAAILNNQLTLFGGEQCSGDGLNDVWQSADGVTWDNLPDAPWEGRGMQINN
                     CVDETGNMWMLSGANEGERRPYSEVWNTADGITWNLINDSPPWMGRYWHTVAWFDNHI
                     WIMGGITAGTELNDVWYSPDGIDWRELKSTTGNWPLGTRHAQSTTVFDNALWYMCGIA
                     TNNAWKIINQSNPINIVTPINHANQITVFPNPTKSTCVVELNDNMIGEKYYIVNILGE
                     ILITGDITNKNQPIDLLNLPSGIYNFIVPSVNTATGIIIKQ"
     gene            5367..6545
                     /locus_tag="PLB46_03240"
     CDS             5367..6545
                     /locus_tag="PLB46_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75559.1"
                     /translation="MRLFTSLFTIIFAIQLQAQSLKLPTDEGVILTADSAMDAALTRD
                     LSAFLAAADLPNEQNIYVLPTQQIETYILLDEILGITKNPKTGQINCYQPVITNCIAL
                     DNNQYLIQVAYMGGDSLPTQLRAIFEIIAFANANSFLFSSPLKYHTHHWNKFTHQNTT
                     VFYKRDIQTKKVEEYATFSTEIDAKLKSTGKLTEFYCCENFVEVLQLIGVLHKADYAS
                     IELNTLGSLVGNKKLIVTGEHSSNFNDFDKHDLWHDRLSLVVPRSTVNKPVDEACAYL
                     YGGSWGYSWDTIFKQFTKRIAISEITDWQYYKENPYDFGESDEKHLYVDYVVNALIVQ
                     HLEKEYGFDAVWKLLNTGKYQKGNANYYTALQQLTGVSADNYNQWVYQLVQAEMNKKQ
                     "
     gene            6619..7773
                     /locus_tag="PLB46_03245"
     CDS             6619..7773
                     /locus_tag="PLB46_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF75560.1"
                     /translation="MFYLIGVVIAFFLVAILLTKRQKTQADWILMGWLSAGGIHLTAY
                     FLFIQNLHLQYPVILPISMPLPLFQGPFLFLYTKALTSKQKLKWQQALHFLPILLAYI
                     MFTPFFLLSFDEKVEVIENEGAPYLTESLINIIAIYISGFVYVIISFITILRYKRNML
                     NQFSNTEKVNYNWLLYLIIWLAVLWIIILFVQYDEVIFGAAVIFIVWLGYFGIKNVQV
                     FSQHKPHAFAGKPDFEMLLADLNNFSGVENADEQLSADTKYVGSVLSKSAIEKIQLGL
                     NELMGNNKIYTNPDLTLDDLADMIKVHPNHLSQVINSVMGKTFYDYINELRVKEFTEQ
                     INLPQKQQFTILAIAFDCGFNSKASFYRNFKKFTGLTPSDYMQMHNKLQN"
     gene            7912..8913
                     /locus_tag="PLB46_03250"
     CDS             7912..8913
                     /locus_tag="PLB46_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF75561.1"
                     /translation="MQTKLVLAGLISLMFFGSCETDHLLDMSGNLVPKTVDEDPDLPS
                     AFVNGVQLHTEAYGPEDGTLLICLHGGPGADYRYLLNCKELADEGYRVVFFDQRGTGL
                     SQRLPEEAYGENVFEAMQLFYDDILGIIAHYRTSPEQKVYILGHSWGGMLGTAFAAHY
                     PNLVEGLVVCEPGGLVWGDIVTYISNSRAFSMWGELMNDATYMDQFLVAEPDDHIMLD
                     YKFGILGANNEITGEDSGLPEGFWRSGAIANKIAFELADVGKPDLSDGIENFHVPVLF
                     IYSELNEAYPDDWAQKISSPFNEVELFKVPGVGHDMLTKQAGWLIAKPKIIEYLNGL"
     gene            8923..9585
                     /locus_tag="PLB46_03255"
     CDS             8923..9585
                     /locus_tag="PLB46_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75562.1"
                     /translation="MKKVLIIVCLLVASKLAKSQNINWALMNDSLNQSVEATLSLDYG
                     ITYGVGYNYVFNTIFPLALHGDFSIPSGENLLDDYKIKTGAVLRLTQIGDVYVDAGLM
                     GITRSYTSDFVRIFNFGSEATLSIGYYRKQWYLGATISYDKAIVSNFKHTDDYKNNYP
                     EVVDGWYEPESGGNIKFGGKASGNFGNNGIVLQAGLVKTEELNSSPLIPFYASLGYTY
                     NW"
     gene            complement(9596..9799)
                     /locus_tag="PLB46_03260"
     CDS             complement(9596..9799)
                     /locus_tag="PLB46_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75563.1"
                     /translation="MLKNTLLYLKIHNLMVNMKIMLRAISFDSYHFTAFEDCNKKNHQ
                     HYAKIEHSVDDLDIINRKLLIIT"
     gene            9818..11497
                     /locus_tag="PLB46_03265"
     CDS             9818..11497
                     /locus_tag="PLB46_03265"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75564.1"
                     /translation="MKKLCIILSLLTSYTTYAQVTQVSDAMYPFDFIVNVNGVLLFFA
                     ADKTTPANYELWRSDGTTAGTYLVKDINGNNDGSILSNGEFGESYTDRDFVVFNDELY
                     FIATDGPHGLEIWKSDGTADGTVMLKDIYPGANGYLTPEFNFPYFTELNDKLYFAATD
                     GVHGFELWKTDGTEAGTQMVIDISADNIYGSNPEHLIAFDGKLYFAARDDVDGYEIFS
                     SDGTAAGTSVVKDIVSGINGSMNNGFASSIDPLFTVSGDYLYFVARVDATLPVLKKLH
                     RTDGTSAGTILLNNDLYDIANLCDVNGTLFCYALDGIFDHSTLWKSDGTETGTEIVNT
                     STVYVSNIGHAFYSFNDLLYFSGFTVDFTEYGLGKSDGTAAGTTMVHGFESVGSIPET
                     YNYNAEPGSNYFFYHALTKISDFEMSVRLVQTNGTDLGTKVFSGAMPFRSTAFLDGDI
                     YFGGIDSTAETVWGLYKITPEELTIPVLNAQATEVLNVFPNPAKDIITIQLPENISEI
                     AVCDMLGNIVTKGKNNTNTLTIDVSDWTAGTYTIFGYQNANLYRCNFEVIK"
     gene            complement(11571..>12209)
                     /locus_tag="PLB46_03270"
     CDS             complement(11571..>12209)
                     /locus_tag="PLB46_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75565.1"
                     /translation="VYNQTSFSSYSLTTIVQDSITNVIPGVESLQLLHPTIAFEWKNK
                     GNKFHEIELTELRFQTQGSDKEVIQTSGTSTIKEVIEGEVTQIIAISARYEYILGANQ
                     TKTKKLVPYIGFGIQPFFEQTKYSPVIATDFPTVSTYTGFRTYITPRLHYFFSEKIYL
                     DVNIPFCVTDLYMSNEVTENPNIPDDQRNNGSNNFRLWPTMYSGRVGIGVVI"
BASE COUNT         3866 a         2003 c         2318 g         4022 t
ORIGIN      
        1 aataaatagg gttgtccggt gctgccatac gttggagaac caattgttgt aatatgttta
       61 taaccatcgg cataaattaa aaaatcttct gcagctgatg ccgtattatt gcttgtaagt
      121 aacactgttg gtacgttcat tctttttgca gtcaattgaa tggtatccgg ataataatca
      181 aattggtaat aataattatc gttagctatt aaatagcttt ttgtcatctc ttctttggac
      241 attccccaat cggggttatc ttgtaaagta tcagccggtg ttaaaaaagt tccccatgct
      301 ttgtaggttg gaatatgcat tctactctca cttctggagc catacatcaa ggtgtcgtct
      361 acaaaatatt ttaagacatt taatccaata tacgtgctgc cacctccatt atttcttaaa
      421 tctataatta cagcttttgc cttatatatt tccggtaata ctgcttgaaa caaactatct
      481 aacgattcgt tttcaaatga attgagttgt aaataggcaa tatcattttc cagtgatttg
      541 taactgagta agttgaaatc ttcaaatgga ggataaattt cctgctcttc agttcgtgca
      601 tgtgtaagtg ttaattgctg tgtgctgcca ttaggttttt tgatggtaat tgtaaattcc
      661 tgcccttcaa aaccttttaa taattgataa acacattgat tttcacgcac ataatctgtt
      721 gatgaggcaa taaatggacg aacatattga gcgatataat ctgcagtagg cattccattc
      781 actgtaataa tttcactgcc agcaggaatt tcatcttttt tactttgatt aactcgggta
      841 ataattgctt taccatcaaa attggttaaa aacaatcggt aatttccaaa catggtagtc
      901 atcatttttt cggataccgc ctgagggtaa tatacattag tatgtccatc ttttagataa
      961 gcacaaaatt gttcgagttt tttataatat tcataatcgt ttggggtggc ttgcacttcc
     1021 attatcataa cctgatacaa actgtcccat tcctttttgt caaccctgtt atagaataca
     1081 aaattgtaat tcacttcctg ccaaaatttg gataagccat atactttttg ctgcggactt
     1141 agtgtgttag gcatttgggc ttgaagtgat agacctgtaa gaaggaggat taggctaaag
     1201 aaagatttca ttgtaaggtt tatttcagtg atgatttaaa attacaaatt tttggtaaat
     1261 caccattttt aggctgggtt tggtgtttta tttaacataa tttaggaatt tggttgttaa
     1321 atataaattg ggcagttagg aacaaacaaa ccaggggcat aaaataaatg gtaaaaaaaa
     1381 gtttagtttt attttacaaa ttaaaaattc tcctaccttt gtgctgaatt aattttcttc
     1441 aatacttcat ttcattacta ttatgtattt catcagggtg cttttttatg ttgtcgcatt
     1501 gacaatagtt gccaattcta tatttgctca aaatattgac aatacaacaa gtcgtttaga
     1561 tagtttgtta aatgcagttg aggtaaggcc atttaacgga gtaattctta ttaagcaaca
     1621 aggcaaagta ctctacgaaa atgcactagg gtttgctgat tacgacaaaa agattccgaa
     1681 taaaatggaa aatgaatttg tgttattgtc taatagtaaa caaataacag cggttttaat
     1741 tttaatggca gcagacgatg ggctgttaca actggaaaat ccaattggac agtatctacc
     1801 tacattatcg atgccatggg ctgatgtaat aaccatacat cagttgttaa atcactcttc
     1861 cggcctagat gctgccgata agccactttt attcgaaccg ggtacatcat ttaagtatgc
     1921 tgatatcaat tatgtcttgc ttggtcaaat tattgaatct gtccgccatc aaacttatgc
     1981 tgatattgca actgcattgt ttaataagtt gggactaaaa cacacctatg ttccaaatgc
     2041 atataatcaa caaaacttaa taattggcaa aaaaatgatt gaaggggaat tacctcaatt
     2101 aattgattct gtaattatat ctcctcataa aattcctgcg gctggtatcg tatcaacagc
     2161 atttgacttt tcaaactggg ttgagttgtt gcacacaggg aaaattttat ctgatactat
     2221 ttatcaaaaa cttattcatt acaatatcac agcgcaacat gttgcatttg ggcctcaaaa
     2281 aattggttat agctacggtt tgcgcattga cgatttaaat gaacctgtct attacggaca
     2341 tacaggcaca tcaccggatc agggttatac ttcgtttacg ttgtggttcc ctaaatctca
     2401 aacaagcgtt gtagtgcttg agaactgtgc ttacgacaac atggatgtaa attattatta
     2461 cgaagcacaa ataagagcaa ttatgaccca aagcaattgg gtgaaatagt tattgataag
     2521 cagactatct aactctactg catctatcaa accaacttaa aggtttacct ttgttacaaa
     2581 ctaacaacac atggcaaccg ttccacattt tggtgatatc gaactcgaca accttagcga
     2641 ttactacgaa gctgattata actaccttgg caaatcaatt gccatagacc tgaattttga
     2701 agatatcact attgaagcaa gcgatttaga gccaaccatt gtcgcgttaa atggattaaa
     2761 taagctcatt gaaacagcct atgccgaatt gaccaaaagt tatcaaacca atggtcctgt
     2821 taaacaatat atcaataggc atatgagtgg cttaaatccc gaggaattag caacattgtt
     2881 tgaagctgca ccggaaggac tcaataagga attaaaaatg atgagtcaat taaaattgcg
     2941 cagaattgga ttttacccag aagataccat cggtttggta agcatggatt ttaccatcgg
     3001 ttatgaactt actgatgatg taattgccat ttattttaat aacaaacttc agttggaaga
     3061 tatagctttg gaaaattaaa aagatgatga gaaaaacgat tataaccatt ttttcggtaa
     3121 ttagtttttt cttgcaattg catgctcaat ctttgagcga tagcctctta gcacattata
     3181 ctttttctga taacttgtta gatggttctg aaaatcacta cgatatttta tccgcatccg
     3241 ggatttttac tacagataga tttggtcaat cgaatgcagc atttgctttc gatggtataa
     3301 atgattcgct tgtgttgccg attcccgaaa tggctccagt atcaggcgat tttgcaatta
     3361 gtttttggta taaaacaaat tcagcaaaaa tcatgaactt gttttcttcc aaacaaacac
     3421 ctgatgatac tactcataat tttgaaatgc agttgaatag tcacaaccaa tattatttgg
     3481 aatattacca acaagtttgg tatcagactt tcgtttactg gaatggaagt ggtgttgatt
     3541 caaatgcatt ggcagaaggg gtggcgggta attttattaa agatgaatgg tgccattttg
     3601 ttattactcg tgaagcagat acatttaaaa tttaccgtaa tcaccaattg tattttttga
     3661 gtattgataa ctactatgga gatgaattag gtgatgctgt tgatttaata ttcagtgcat
     3721 cgccataccg tttcaaaggt gcagttgatg atatacgttg ctacaatcgc aacctgacac
     3781 aacaagatgt tgatgcgtta tggtttgaaa ataatcctat tcaatttgta acaccacagg
     3841 caacagatgc ttatgtattt ggctctaatg tattggttta ctgggaatat gataccgcgc
     3901 aaatcagcga ttcaattttg gtgcagtatc gaattaataa tggcgattgg ataaatagta
     3961 tacactctaa tttaatttgg gaaaattata cttatatcga tatgagttat gagccgggca
     4021 ctgctgttga agtgcgtgtt gcagattata ctgataccag tatttttcaa ttaacaggac
     4081 aatttattgt aagcccttac gattggattt cagtaaatga tagtttgcca tttgccgcaa
     4141 aagatggagc aggattggta tcatttaaaa ataaaatgtg gttattaggt ggatgggatc
     4201 ctccatttca cgaaccaaat tatacgcaca atgaagtatg gagttcaata gatggcgtca
     4261 catggaattt tgaaactttg gcaccttggc caccgcgaca ttgcgctgcc tggttaagtg
     4321 atgaaaatta tatctatgta attggtggcg atcctcaatc cggctgttta actgatgtat
     4381 ggcagtcggc agatggcatt aattgggtgc aattagtaga taccattccc ggttataaaa
     4441 aacgtaatat ggtcaacgct gcaattttga ataatcaatt gacattattt ggtggggagc
     4501 aatgcagtgg cgatggatta aatgatgttt ggcaaagtgc tgatggtgtt acctgggata
     4561 atttacctga tgcaccatgg gaaggaagag gtatgcaaat aaataattgt gttgatgaga
     4621 ccggtaacat gtggatgctg agtggagcaa atgaaggtga gcgaagacca tatagtgaag
     4681 tatggaatac agcagatggc attacttgga atttaataaa tgattcaccc ccatggatgg
     4741 gcaggtattg gcataccgtt gcctggttcg acaatcatat ttggattatg ggtggaataa
     4801 ctgcaggcac tgaattaaat gatgtgtggt attcgccgga tggcattgat tggagagaat
     4861 taaaaagtac taccggaaat tggccattag gaacaagaca tgcacaatcc accaccgtat
     4921 tcgacaatgc actctggtat atgtgtggta tagcaacaaa taatgcatgg aaaattatta
     4981 atcaatctaa tcccattaat attgtaacac caattaatca tgctaatcaa attactgttt
     5041 tcccaaaccc gacaaaatct acttgtgtag ttgagcttaa tgataatatg attggtgaaa
     5101 aatattacat agtaaatata ctaggagaaa tattaatcac aggtgatatc acgaataaaa
     5161 atcaacctat tgatttattg aacctgcctt ccggaattta taatttcata gtgccaagtg
     5221 taaatactgc tacaggtatt atcatcaaac aataatgttc gaaaatttaa caattaatgg
     5281 catattattt gcgccattga tttgattttg cagctatgta ataattagtt gcctgatatt
     5341 ttttcgtcac tactcaacca actcacatgc gtttatttac atcgctgttt actattattt
     5401 ttgccattca attacaggca caatcgctta aactacctac tgatgaaggt gttatactca
     5461 ccgcagattc ggctatggat gccgcattaa ctcgcgattt atctgcattt ttagctgcag
     5521 ccgatttgcc aaacgaacaa aatatttatg tgctaccaac acaacaaatt gaaacatata
     5581 ttttattgga tgaaatttta ggtatcacaa aaaatcccaa aactggacaa attaattgct
     5641 atcagccagt tataacgaat tgtattgcac tggataataa tcagtattta atacaagttg
     5701 cctatatggg aggtgattca ttgccaacac aattgagggc catttttgaa ataattgcgt
     5761 ttgccaatgc caatagtttt ttattctctt caccactaaa atatcataca catcactgga
     5821 ataaatttac acatcaaaat acgacagtct tttataaacg cgatattcaa acaaaaaaag
     5881 tagaagaata tgcaacattt tctaccgaga ttgatgctaa attaaaatct acaggtaagc
     5941 taaccgaatt ttattgttgt gaaaattttg tagaagtatt gcagttaatt ggggtgttgc
     6001 ataaagctga ttacgcaagt attgagttaa atactttagg ttcattagtt ggcaataaaa
     6061 aattaattgt aacaggtgaa cacagcagca atttcaacga ttttgataaa cacgaccttt
     6121 ggcacgacag gttgagtttg gtagtgccaa gaagcacggt aaacaaacct gttgatgaag
     6181 cctgtgctta tttatacggt ggaagttggg gatatagttg ggatacaatt tttaagcagt
     6241 tcacgaaaag aattgctatc tccgaaatca cggattggca atattacaag gaaaatcctt
     6301 atgactttgg cgagtccgat gaaaaacatt tatatgtaga ctacgtggta aatgcactca
     6361 ttgttcaaca ccttgaaaaa gaatatggtt ttgatgccgt ttggaaattg ctcaataccg
     6421 gtaaatacca aaaaggaaat gctaactatt atactgctct gcaacaatta accggagtat
     6481 cagcggataa ttataaccaa tgggtgtatc aattggtaca agcagaaatg aataaaaagc
     6541 agtaagtttt ccctaactgt aaaggtattg gatttagcat aataattcta ttacctttac
     6601 acctgtatca aatcaggtat gttttattta ataggtgtag taattgcgtt ttttttagtc
     6661 gctattttat tgactaagcg gcagaaaacc caggcagatt ggattttaat gggttggctg
     6721 tctgcaggtg gtattcactt aactgcttat tttttattta ttcaaaacct ccatcttcag
     6781 tatccggtaa ttctacctat ttcaatgccg ttacctttgt ttcagggccc ctttttattt
     6841 ttatatacaa aggcgcttac cagcaaacaa aaattaaaat ggcagcaagc tttgcatttt
     6901 ctgccaatct tattagcgta tattatgttc acaccttttt ttctattgtc gtttgatgaa
     6961 aaagttgaag tgatagaaaa tgaaggtgca ccgtatttaa ctgaatcgct gattaatatt
     7021 atcgctattt atatttccgg ttttgtttat gttatcattt cgtttataac cattttgcgc
     7081 tataagcgga atatgctcaa ccaattttcc aataccgaaa aagtaaatta taattggttg
     7141 ttatatctca ttatatggtt ggcagtttta tggataatta tcctatttgt acaatacgat
     7201 gaagtgattt ttggtgccgc ggtaatattt atcgtttggt taggctattt cggtatcaaa
     7261 aatgtgcagg tgtttagtca gcataaaccg catgcatttg ccggcaaacc tgatttcgaa
     7321 atgcttttag ctgatttaaa caatttttcc ggagtagaaa atgcggatga acaattatcg
     7381 gccgatacta aatatgttgg ctccgttttg tcaaaatctg cgattgaaaa aatacaactg
     7441 ggtttgaacg aactgatggg taataataaa atttatacca accccgacct caccctcgac
     7501 gatttggcgg atatgataaa ggtgcatcct aatcatttat cgcaggtaat aaattctgta
     7561 atgggaaaaa ctttttacga ttacatcaat gagttacgcg ttaaagagtt taccgaacaa
     7621 atcaatctcc cccaaaaaca acagtttacc attttggcaa ttgccttcga ttgtggattt
     7681 aattccaagg cttcgtttta tcgcaacttc aaaaaattta cgggacttac accttccgat
     7741 tacatgcaaa tgcataataa attgcagaat tgagacgtct catcgtttta ttttgtctaa
     7801 ctgtcactaa atcaataagt tgtaatttaa atttttgatg ttccatctta cccgttgaga
     7861 cctttagtga tataattccc tccaattttg cagtattaaa aatcaaaatt tatgcagaca
     7921 aaattggtgt tggctggatt aatcagccta atgttttttg ggtcatgtga aactgaccat
     7981 ctattagata tgtccggaaa tttggttcct aaaaccgtag atgaggatcc ggatttgcct
     8041 tccgcattcg taaacggagt gcaattacat accgaagctt atggtcctga agacggtact
     8101 ttattaattt gtttacatgg aggtcccggc gccgattatc ggtatttatt aaattgtaaa
     8161 gaacttgcag atgaaggtta tcgcgttgtt tttttcgacc agcgcggaac tggtttatct
     8221 caacgcttac ctgaagaagc ctatggggaa aatgtatttg aggcgatgca gctattttat
     8281 gatgacattt taggcatcat agcacattac agaaccagtc ccgaacaaaa agtatatatc
     8341 ctcggtcact catggggcgg catgttggga acagcgtttg cagctcatta tcctaacttg
     8401 gtggagggtt tggtagtatg tgaaccaggt ggtttagttt ggggagatat tgtcacctac
     8461 atctccaact cacgcgcctt tagcatgtgg ggagaattaa tgaatgatgc cacttatatg
     8521 gatcaatttt tagtcgccga acccgacgat catatcatgt tggattataa attcggtatc
     8581 ctgggtgcga ataatgaaat aacaggtgaa gattccggat tacctgaagg attctggcgc
     8641 tctggtgcaa ttgccaataa aattgcgttc gaattagccg atgtaggcaa gccggattta
     8701 tctgatggaa tagaaaattt tcatgttcct gttttattta tttatagcga attaaatgaa
     8761 gcataccctg atgattgggc gcagaaaata tcatctccct ttaatgaagt ggaattattt
     8821 aaagtgccgg gagttggaca tgatatgtta actaaacaag ccggttggtt aattgctaaa
     8881 ccaaaaatca ttgaatattt aaacggatta taaattgtat atatgaaaaa agtattgata
     8941 atagtttgtt tgctcgttgc atcaaaattg gctaagtctc aaaacataaa ttgggcatta
     9001 atgaacgata gtttaaatca atcggttgag gctactctat cattagatta tggcattaca
     9061 tacggagtgg gatataatta tgtgtttaat acgatattcc cactggcact tcatggcgat
     9121 ttttcaattc cttccggtga aaatttattg gacgattata aaataaaaac aggcgctgtt
     9181 ttgcgcctga cacaaattgg cgatgtgtat gtcgatgcag gcttgatggg tatcacaaga
     9241 agttacacgt ctgattttgt gcgcattttt aattttggtt ctgaagcaac tttaagtatc
     9301 ggatattacc gtaaacaatg gtatttagga gcaactatca gttatgataa agcaatagtt
     9361 tctaatttta aacatactga tgattacaaa aataactacc cggaagtagt tgacggttgg
     9421 tatgaaccag aaagcggtgg taatattaaa tttggtggaa aagcaagcgg taattttggc
     9481 aataatggaa ttgtattgca agccggttta gtgaaaacag aagaactaaa ttcttcacca
     9541 ttaattcctt tttatgccag tttaggatat acatataatt ggtaagttgt tcttcctaag
     9601 taataattag tagttttcta ttaataatat ccaaatcatc aacgctatgt tcaattttag
     9661 catagtgttg atgatttttt ttgttacaat cttcaaaagc tgtaaagtga tacgaatcaa
     9721 atgaaatagc cctcagcata attttcatat ttaccatcaa attgtgtatt tttaaataca
     9781 ataaggtgtt tttcagcata actcaaatta tccatttatg aaaaaactat gtataatcct
     9841 ttcgctctta actagttata caacttatgc gcaagtaact caggtaagtg acgccatgta
     9901 tccttttgac tttattgtca atgtgaatgg ggttttgttg ttttttgccg cagataaaac
     9961 aacacctgca aattatgaat tgtggcgttc tgatggcact acggcaggca catatttagt
    10021 aaaggatatt aatggtaata acgatggaag tattttatct aatggagagt ttggagaaag
    10081 ttatactgac cgtgattttg tcgtttttaa cgatgaatta tattttattg ccacagacgg
    10141 accgcatgga ttagaaatat ggaaatcgga tggcactgct gatggaacgg tcatgttaaa
    10201 agatatttat ccgggtgcaa atggttatct cacacctgaa tttaattttc cgtatttcac
    10261 tgaacttaat gataaattat attttgccgc aaccgacggt gtacatggtt ttgaattatg
    10321 gaaaacagat ggcactgaag ctggtacaca aatggttatt gatatttctg ctgataatat
    10381 ttatggttca aatccggagc atttaatcgc atttgacggg aaactttatt ttgctgcaag
    10441 ggatgatgtt gacggatacg aaattttttc gagcgatgga acagcggcag gaacaagtgt
    10501 ggtaaaagat attgtttccg gaattaatgg ttcaatgaat aatggttttg caagtagtat
    10561 tgaccctcta tttacagtga gtggcgacta tttatatttc gttgcccgtg tagatgcaac
    10621 attgcctgtg ttgaaaaaat tgcacagaac agatggcaca tctgcaggaa caattttact
    10681 aaataatgat ttatatgata tcgctaattt atgtgatgtc aacggcactt tgttttgtta
    10741 tgcattagat ggcattttcg atcattctac attatggaaa tcggatggta ctgaaacagg
    10801 cacagaaata gttaatacca gcacagttta tgtatccaac atcggtcatg cattttatag
    10861 ttttaacgac ctgctttatt tttcaggatt tacagtcgac ttcaccgaat atggcttagg
    10921 aaaaagtgat ggcacagctg caggaacaac gatggtacat ggatttgaaa gtgtgggtag
    10981 tattcctgaa acatataact ataatgcaga acctggaagt aattactttt tttatcatgc
    11041 attaactaaa atttctgatt tcgaaatgtc tgtccgtctg gtgcaaacaa acggaactga
    11101 tttaggaaca aaagtatttt caggtgccat gccatttcgc agcaccgcat ttttagacgg
    11161 cgatatttat tttggcggaa tagatagcac tgcagaaaca gtttggggtt tatataaaat
    11221 aacacctgaa gaattgacca ttcctgtttt gaacgcacaa gcaactgaag tgctgaatgt
    11281 ttttccaaac ccagcaaaag atataatcac tattcaattg cccgaaaata taagtgaaat
    11341 cgctgtatgt gatatgttgg gtaatattgt taccaaggga aaaaataata ccaatacttt
    11401 aaccatagat gtttcagatt ggaccgcagg tacatataca atctttggtt accaaaatgc
    11461 aaatttgtat agatgtaatt ttgaagtaat aaaataacac atcgatattt ccattgtatt
    11521 ttttcaaaaa tactttgaac gactacaatg gaaatatacc tttgttacat ttaaatcacc
    11581 accccaatcc ctacacgacc gctatacatg gttggccaca aacggaaatt gttggagcca
    11641 ttgttgcgct ggtcatccgg aatatttgga ttttcggtaa cttcattact catatacaaa
    11701 tcagttacac aaaaaggaat atttacatcc agatatattt tttcactgaa gaaataatgt
    11761 aatcttggtg ttatgtatgt gcgaaaaccg gtgtaggtag acacagttgg aaaatctgtt
    11821 gcaattaccg gactatattt tgtttgttcg aaaaaaggct gaataccaaa tccgatataa
    11881 ggcactagct tttttgtttt tgtttgattg gcgcccaaaa tatattcata tcgtgcactt
    11941 atggcgataa tttgagtaac ctcaccttca atcacttctt tgatagtaga ggttccgctg
    12001 gtttgtatca cctctttatc actaccttgt gtttgaaaac gcaattcggt aagttcaatt
    12061 tcatggaatt tattaccctt atttttccat tcaaatgcaa tggttggatg aagaagttgt
    12121 agcgattcaa caccgggaat tacattagta atactatcct gaactatggt tgtcagagag
    12181 taactgctaa aactcgtctg attatacac
//
LOCUS       DAORKW010000043        12163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_89_length_12163_cov_4.821606, whole genome shotgun sequence.
ACCESSION   DAORKW010000043 DAORKW010000000
VERSION     DAORKW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89_length_12163_cov_4.821606"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2041)
                     /locus_tag="PLB46_03275"
     CDS             complement(<1..2041)
                     /locus_tag="PLB46_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRF75566.1"
                     /translation="MAKKKTDNNDEEIEKSENITPAQVKTSVTHVSGMYKDWFLDYAS
                     YVILERAVPDIRDGVKPVQRRILHAMREMDDGRFHKVANVIGQTMQYHPHGDAAIGDA
                     LVNMGQKDLLIETQGNWGDVRTGDDAAAPRYIEARLSKFALEVVFNKETTEWQLSYDG
                     RKAEPVFLPVKFPLVLAMGVEGIAVGLSTKILTHNFCELILASIEVLKGNPTKILPDF
                     ANGGFIDVTDYNSGERGSKIRCRAKMEVADKKTILIKEIPYGTTTSSLIDSIIKANDK
                     GKIKIKKVTDNTAANVEIAIELAAGVSPDVTVDALYAFTDCEISISPSTCVIEDDTPL
                     FLTVDEILRRSTKNTKNLLKLELELRLAELNEKWHFSSLEKIFIENRIYRDIEECETW
                     EAVIKTIDKGLNPFKKLLVREVTEEDIVRLTEIKIKRISKFDAFKADEYIRALENEMK
                     EVKHNLRHLTDYAIKYFEDLLKKYGKGRERKSEIRKFDTIEAKAVVANNAKLYVNREE
                     GFVGFGLKKDEFICDCSDIDDIIAFTDDGKMKVVRIADKVFIGKKILYAAVWKKEDER
                     MTYHMLYSDSKTGKAFAKRFNVTAITRDKEYDLTQGNPKSKVWYFSANPNSESEVINI
                     QLHPNAKAKIKIFDFDFGELAVKGRSSMGNTVTKYPLRKAVQKERGTSTLGGRKIW"
     gene            2161..3306
                     /locus_tag="PLB46_03280"
     CDS             2161..3306
                     /locus_tag="PLB46_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75567.1"
                     /translation="MHKLTAYSTAAAAFIACANAAQGQIVYQNIDPDLVTEDERIFLD
                     VNADGEFDIWFDLFHSGFFGAQYTSAYAGNQNPYVQMATNGLLPGAQGLKNFAEGEII
                     DSAANWETPAPFFGDLLGRLLFFDYYSCEDMLVYFPYGEFVDVSNGILAFKIEDAGEN
                     YYGWVRLSISQSPVCPLPLYDALTIVVHDFAIQNTPNAPIAAGVVGCDTPENPDAEAA
                     ATAIKVSWDAADGATEYKIQYRPVGAISWETKTASAPKTARIIKGLDCNTTYEYQIAG
                     ICDGIVSAFTSIQTITTLDCKIGDMPLTETLLIFPNPATDVITINIDEEIVADNISVT
                     DLQGNIVLQRNLPANSMVIDISGLANGTYIIIAQTPEGMQHAYFVKQ"
     gene            3484..3813
                     /locus_tag="PLB46_03285"
     CDS             3484..3813
                     /locus_tag="PLB46_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75568.1"
                     /translation="MKSKLSKLYVALFLLLGFAYASCGDFPFGGFGDVDPDLTDSIYD
                     DPTDSIWIDDDSTEIDFPGDSIDIEWPEDSTEWPEDSTFWPEDSIFWPEDSIFWPEDS
                     VFEDSIP"
     gene            3893..4522
                     /locus_tag="PLB46_03290"
     CDS             3893..4522
                     /locus_tag="PLB46_03290"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF75569.1"
                     /translation="MHTIKQIHNERITLPIKDNTFVCSQNMGITPELLTKSATGNKQA
                     QYLLYKQSYSLLMSICARYVKNFDDAMALLNEGFLKILLHADKRKSNVPPELWMRRIM
                     INTIIDAYRKNKQYKEQTQLQDFSDQSFGDVNNDTTENEYEHHIQSETLQHMLNKLPE
                     VTGKVFNLFAIDGFTHKEIAEMLQMSEGTSKWHVNNARTLLKQMINELD"
     gene            4534..6141
                     /locus_tag="PLB46_03295"
     CDS             4534..6141
                     /locus_tag="PLB46_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75570.1"
                     /translation="MSDKRNDNNKQNPIDKVFRDGLHDRPFEFDEKYWEQAQGTLSQF
                     DQLKKSGKGGNRGLFWMLGGALVVIGFLAWFLLKPETQPIASTSSNNQQQKNITSENK
                     TTEKVIGEKAELPQQQQTPVISSQNDEKIGNNLQVVDNQSNKIENNQQIGDKQSKNDG
                     SKGKQDNQNPLANTGSKNSNKATDNNDKTTTANNTTSDGASRQIDVKQEDKLASNNDV
                     VGVNENQLGEQNLANNQITTKEDIIDNQTQKADTGNTDNNNSNDVTAEEKAIISIDSN
                     QITSLEKSDSTQTNVIPSNKTTPKKSTNLDLNWYGALSFGADFTSTTLSSTNPSLTEW
                     VNYRTAGETLTPTLGVALEGGLNIKQFTVGTGINMFNYASDVQYSFLSVNIDSVIEYV
                     YDTAMVTIIDSFYVISIDTNEQVFNGRNKISYIEIPILLGYQFKAGNWRFGLSTGPSI
                     GLLNSAVITYPNYDLTTTETKPVTYFKNSYFNWIAKPSVTYMFTDNIGFGINGMFRWN
                     LGSIVEDADIQQQYSGYGIQVGLRVEF"
     gene            complement(6205..8079)
                     /locus_tag="PLB46_03300"
     CDS             complement(6205..8079)
                     /locus_tag="PLB46_03300"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRF75571.1"
                     /translation="MATTNYNYSEDHIRTLDWIEHMRMRPGMYIGKLGDGSSPDDGIY
                     ILVKEVIDNAIDEYMMGYGKKIEVEIEDYKVTVRDYGRGIPLGKVIECVSVMNTGAKY
                     DSAAFQKSVGLNGVGTKAVNALSHYFRVEAFREGKTKMAEFEKGILRKDNAIIKSDQK
                     DGTLVTFIPDNTIFKNFHFIPEYLDNQLWNYVFLNAGLTIHYNGQKYYSQNGLLDLLK
                     RKTNEDDLRYPIIHLKGDDIELSLTHNNEYGEEYYSFVNGQFTTQGGTHLAAFREGIV
                     KTVREFYKKDFDASDIRQAIVGAIAIRVQEPVFESQTKTKLGSINMSPDGVTVKSFVV
                     DFIKKELDNYLHKNTATADALLKRILQSERERKDIAGIKKLANERAKKANLHNKKLRD
                     CRFHLGEDKHEKTINSTLFITEGDSASGSITKARDVETQAVFSLKGKPLNVYGLTKKI
                     VYENEEFNLLQHALNIEDGIEGLRYNQIVIATDADVDGMHIRLLLLTFFLQFFPDLVK
                     NGHVYILQTPLFRVRNKKSTIYCYSETEKQNAVNQLGNKPEITRFKGLGEISPDEFSG
                     FIGESIRLEPVIIGKEQTIQSTLEYYMGKNTPERQGFIIKNLRVEKDVIEEAEVVAEV
                     "
     gene            complement(8313..9662)
                     /locus_tag="PLB46_03305"
     CDS             complement(8313..9662)
                     /locus_tag="PLB46_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRF75572.1"
                     /translation="MALKKKAKVQEEKKIFILDTSVILHDHNSVNKFEEHDVVIPITV
                     LEELDNFKKGNETINFEAREFIRFLDNKSKESTLQEWRPINGRTTGRMLVAMYAHTGL
                     NAEEVFGEEKPDHKILNTALVMKQKYPEHKVILVTKDINLRLKAKSLGLYSEDYKNDK
                     IENTDLLKEPIETIEGIDHESIDKIYSDGFIGFKKILKQEPFANQCFILKNGSSSVLA
                     SYNSEEKVFERINKATAYGIKPRNAEQVFALNVLLDPDIKLVALQGQSGTGKTLLALA
                     SALEQRRNYKQIFLARPIIPLNNKDIGYLPGDIKSKLNPYMEPLWDNLKMIQNQFEQN
                     DSEYKAIAQMIEQEKLVIQPLAYIRGRSISNIYFIIDEAQNLTPLEVKTIITRAGENT
                     KIIFTGDINQIDTPYLDTHSNGLSYLIDKINNHPMCSVITLQKGERSELANLANELL"
     gene            complement(9759..11399)
                     /locus_tag="PLB46_03310"
     CDS             complement(9759..11399)
                     /locus_tag="PLB46_03310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HRF75573.1"
                     /translation="MPLNTLTRAANMHAAANTQFDVIIIGGGITGAGIALDAASRGLK
                     VLLLEKYDFAGGTSSKSTKLIHGGLRYLKQLEFGLVRTVGIERAVIHKLAPHLVEPIN
                     MVLPIYKNGSLGKFSTGIALRIYDTLAKVERDDRYYMVDPTHLHQLEPLLSTRDLIGG
                     AIYKEFRTDDARLTISVIKSAATFGARCFNYMKVENFIFTNNIINGVVARDLLSDTTI
                     TFNATHVINAAGPWVDDVRSLETLPKGKKLHLTKGIHIVVNKNDFPVQHALYFDVAGD
                     NRMIFVIPRGKSVYIGTTDTNYVGDKDNILLSREDVNYLLDAVNNKFPKVQLQYSQVI
                     SGWAGLRPLIHEEGKSPSELSRKDEIFISDKGLISIAGGKLTGYRKMAEKVVNMLAKS
                     FKKSGRKIPNSKTHELLLFGADFDCSIPEYITRRTGEAKQIDVDENVISYLVYTYGTG
                     SETIINYAFDIGNAIKNPASRLLTAEIMYAIEHEMVTNVSDFIIRRTGRLYFDMEQTK
                     QLYPEIAKIIAAKLSLDEVEAAQQLAFFETALVQALPA"
     gene            11489..>12163
                     /locus_tag="PLB46_03315"
     CDS             11489..>12163
                     /locus_tag="PLB46_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75574.1"
                     /translation="MSKIRIICLCILFLGVNKIYTQVPTLDWAGKIGKNGSDYGYQVH
                     AGLDGNIYAVGTFKGNNVDFNIDTAAAEVDVFSSGNYNGSILVKYTKTGEFLGGFSLH
                     SDNDMVINGTVTFNSIYVESDGEIYVTGFYQGTTDFDPGVGENILTAIGDADIFIAKY
                     SGDFTLQWVKRIGYAGRLSGGEKLVLGNDGAIYVTGYFGGVCDFNPDDIAVNNLTSLG
                     TDDAFIA"
BASE COUNT         3677 a         2333 c         2186 g         3967 t
ORIGIN      
        1 accagatttt tctaccacct aatgttgaag ttccgcgttc tttttggaca gcttttcgca
       61 gtggatattt ggtaaccgta ttccccatac tggaacgtcc ttttacagcg agttcaccaa
      121 aatcgaaatc gaatattttt attttggcct tagcatttgg gtgcagctga atattgataa
      181 cttcactttc gctattcgga ttggcagaaa aataccaaac tttacttttc ggattgcctt
      241 gcgtaaggtc gtattcttta tcacgagtaa tggctgtcac attaaatcgt ttggcaaaag
      301 cttttcctgt tttgctatca gaatacaaca tatgataagt catacgttca tcttcttttt
      361 tccagacagc agcgtataat atttttttac cgataaatac tttatccgca attcgcacta
      421 ctttcatttt accatcatca gtaaaagcga taatatcatc aatatcggaa caatcacaaa
      481 taaactcatc tttctttaaa ccgaaaccga caaaaccctc ttccctgttg acatacagct
      541 tagcattgtt ggccacaact gctttagctt caatggtatc gaatttgcgg atttccgatt
      601 tacgttcgcg acctttgccg tattttttta ataaatcttc aaaatattta atcgcataat
      661 cggtgaggtg acgaagatta tgtttcactt ctttcatctc attttctaat gcccgaatat
      721 attcatccgc tttaaaagca tcgaatttcg aaatgcgttt gattttaatt tcagtaagtc
      781 ggacaatatc ttcttccgta acttcacgca ccaataattt tttaaacgga tttaatcctt
      841 tgtcgatggt tttaattacc gcttcccagg tttcacattc ttctatgtcg cggtaaattc
      901 tattttcaat aaatattttt tccaaagaac tgaaatgcca tttttcgttt aattcggcaa
      961 gtcgcaattc caattccagt ttaaggaggt tcttcgtatt ttttgttgag cgacgaagaa
     1021 tttcatctac cgttaaaaac aaaggtgtat catcttcaat aacacatgtt gatggagaaa
     1081 tcgaaatttc gcaatcggta aatgcataca aggcatccac agtaacatca ggcgaaactc
     1141 ctgctgctaa ttcgatagca atttcaacat tggccgcagt attatccgtt acttttttaa
     1201 tttttatttt ccctttgtcg ttggctttga ttatagaatc aattaacgac gaagtggtgg
     1261 taccatatgg tatttcttta attaaaattg tttttttatc cgcaacttcc atttttgctc
     1321 ggcaacgaat tttgcttcct ctttctccac tattataatc ggtaacatca ataaaaccgc
     1381 cgttggcaaa atcgggtaat atttttgttg gattgccttt gagtacttct atactcgcta
     1441 atatcagttc acaaaaatta tgggtaagaa ttttagtaga taaaccaaca gcaataccct
     1501 caacacccat ggcaagcacc aatggaaatt taacaggtaa aaaaacaggt tctgcttttc
     1561 tgccatcgta acttaattgc cattcggtag tttctttatt aaaaactact tccaatgcaa
     1621 acttcgacaa acgtgcttct atataacgtg gtgccgctgc atcatcaccg gttcttacat
     1681 caccccagtt accctgtgtt tcgatgagta aatctttttg gcccatattc acaagggcat
     1741 caccaattgc agcatcacca tgcgggtgat attgcatggt ttgaccaatt acgttagcta
     1801 ccttgtggaa acgaccatcg tccatttcgc gcatggcatg cagaatacgt cgctgaacgg
     1861 gttttacccc atcgcggata tcgggcacag cgcgttccag gatgacataa ctcgcgtagt
     1921 cgagaaacca atccttatac atgcctgaaa catgcgttac actcgtttta acctgcgcag
     1981 gagtaatatt ctccgatttt tctatttcct cgtcgttgtt gtctgtcttc ttctttgcca
     2041 ttgcaattaa cgtaaacccc aaaagtaaac attagtaaca gagcctaaac cacaagtttt
     2101 ccacgttgtt ttttgcacat gtaggctgac tttattatct taacagtaaa aattaaccat
     2161 atgcacaaac tcaccgccta ttctaccgca gctgctgcat ttattgcctg tgccaatgcc
     2221 gctcaaggac aaattgttta ccaaaacata gatcctgatt tggtgactga agatgaacgt
     2281 atatttttag atgtaaatgc ggatggagaa tttgacatct ggttcgattt atttcacagt
     2341 ggattttttg gtgctcaata tacttcagcc tatgcaggca atcaaaatcc gtatgtgcaa
     2401 atggctacta acggattatt acctggtgct cagggattaa aaaattttgc tgaaggtgaa
     2461 ataattgaca gcgccgccaa ctgggaaaca cctgcaccat tttttgggga tttattgggg
     2521 agactattat ttttcgatta ttatagttgt gaagatatgc tggtatattt tccttacgga
     2581 gaatttgtgg atgtcagcaa tggtattttg gcatttaaga ttgaagatgc aggcgaaaac
     2641 tattacggat gggtgcgact tagtatatca caatctcctg tctgcccatt gcccttatat
     2701 gatgcactca ctattgttgt gcatgatttt gccatacaaa atacacctaa tgcaccaata
     2761 gctgccggag tggtgggatg cgacacccct gaaaatccag atgcagaagc tgcggcaact
     2821 gctattaaag taagctggga cgctgctgac ggagctacag aatataaaat tcaataccgc
     2881 cctgttggag caatatcctg ggaaacaaaa accgcctctg ctcccaaaac cgcacgtata
     2941 ataaaaggtt tggattgtaa taccacttat gaatatcaaa ttgccggtat ttgcgatggg
     3001 attgtttctg cttttacttc tattcaaaca attaccacac ttgattgtaa aattggggac
     3061 atgccattga cagaaacatt gttgattttt ccaaatcctg caacagatgt tatcactata
     3121 aatattgatg aagaaattgt tgcggataat atttcggtta cagacttaca aggtaatata
     3181 gtattacaac gcaatttgcc agcaaacagt atggttatcg atatttcagg gcttgcaaac
     3241 ggcacttaca tcattatagc acaaactccg gaaggcatgc aacatgctta ttttgtgaaa
     3301 caataatttt tgtttactgc ggtagccttg cattcatatt gagtgagtgt taattatgtg
     3361 aagcgaaaaa aaagctcatt acccaaccta attaaaattt cttacgtgta ggtaatcaaa
     3421 agcaacgatt ctacattgaa ttgagctttt tgttttttaa ttaatcttaa accttattaa
     3481 attatgaaaa gtaaactgag caagctgtat gttgcccttt ttctcctctt aggttttgcc
     3541 tatgcgtcgt gtggcgattt cccttttgga ggatttggtg atgttgaccc tgatttaacg
     3601 gactcaattt atgatgatcc gacagattca atctggattg atgatgattc caccgaaatc
     3661 gattttccag gcgattccat tgatattgaa tggccggaag attcaactga gtggccagag
     3721 gattcaactt tttggcctga agattcaata ttctggcccg aagattcaat attttggcct
     3781 gaagattctg tattcgaaga ttcaattccg taaatgctga taggttccat cctgcttctt
     3841 attaggcagg atggtttctt taatatttgg cgggttgatt aagttaaaaa tcgtgcacac
     3901 cataaaacaa attcataatg agcgtataac tttaccaatt aaagacaaca catttgtctg
     3961 tagtcaaaac atgggcataa cgccggaatt attaaccaaa tcagcaacgg ggaataaaca
     4021 agcccaatat ttattataca agcagagtta tagcttgttg atgagtatat gtgctcgtta
     4081 tgtcaaaaat tttgatgatg ctatggcatt actgaatgaa ggttttctaa aaattttact
     4141 acacgccgat aaacgcaaaa gcaacgtgcc gccagagttg tggatgcgcc gcataatgat
     4201 taataccatt atagatgcgt atagaaaaaa caaacaatat aaagaacaaa cacaattaca
     4261 ggatttttcc gatcaatctt ttggagatgt aaataacgac actaccgaaa atgaatatga
     4321 acaccatatt caaagtgaaa cattacaaca tatgttgaat aaattacccg aagtaaccgg
     4381 taaggtattt aacctttttg ctattgatgg gtttacccat aaagaaattg ctgaaatgct
     4441 tcaaatgagt gaaggcacca gcaaatggca tgtaaacaat gcccgaacat tattaaaaca
     4501 aatgattaac gaacttgatt aagtttttac gttatgtcag ataaaaggaa cgataacaac
     4561 aagcaaaatc cgatagacaa agtctttcgc gatggtttgc acgaccgtcc ttttgagttt
     4621 gatgaaaaat actgggaaca ggcacagggc acactcagtc agtttgatca acttaaaaaa
     4681 agcggtaaag gtggcaacag aggtcttttc tggatgcttg gcggagccct tgtagttatc
     4741 ggctttttag cctggttttt attaaaaccg gaaactcaac ctattgcatc aacatcatca
     4801 aataatcaac agcaaaaaaa tataacaagt gaaaataaaa caacagaaaa agtaattggg
     4861 gagaaagcag aattaccaca acaacagcaa acgcctgtaa taagttccca aaatgatgaa
     4921 aaaattggaa ataatttgca agttgttgat aatcaatcaa ataaaattga aaataatcag
     4981 caaattggtg acaagcaatc gaaaaatgat ggttctaagg ggaagcaaga caatcagaac
     5041 ccactggcaa atacaggttc caaaaacagt aataaggcaa cagacaataa cgataaaaca
     5101 actactgcta acaacacaac aagtgatggc gcttcaagac aaattgatgt aaaacaagag
     5161 gataagttgg catcaaacaa cgatgttgtg ggagtaaatg aaaatcaatt aggtgagcaa
     5221 aatttggcaa acaatcaaat aacaacgaag gaagatatta ttgataatca aacccaaaaa
     5281 gctgataccg gtaataccga taataataat tccaatgatg ttactgctga agaaaaagca
     5341 attatatcaa ttgacagtaa tcagattact tctttagaaa aatcagatag tactcaaacc
     5401 aatgttattc catccaataa aaccacgcca aaaaaatcga caaaccttga tttaaactgg
     5461 tatggcgctt taagttttgg tgcagatttt acatctacta ctttatcaag taccaatcct
     5521 tcattaactg agtgggttaa ttatcgcaca gcaggtgaaa cactaacacc aactttaggt
     5581 gtagcattgg aaggcggatt aaatataaaa cagtttacgg ttggcaccgg aattaatatg
     5641 tttaactatg caagcgatgt acaatattcg ttcttgtctg tcaacatcga ttctgttatc
     5701 gaatatgttt atgacaccgc tatggttaca attattgatt cattttatgt gatttccatc
     5761 gatacaaacg aacaagtatt taatggacgt aataaaatca gttatatcga aatacctatt
     5821 ttattgggtt atcagtttaa ggcaggtaat tggcgttttg gattgagtac aggtccatcc
     5881 attggattat taaacagtgc cgttattact tatccgaatt atgatttaac aactactgaa
     5941 acaaaacctg ttacctattt caaaaacagt tatttcaact ggattgccaa accttcggtt
     6001 acctatatgt ttactgacaa tattggtttt ggcataaatg gtatgtttag atggaatttg
     6061 ggctctattg tagaagatgc cgacatccag caacaatatt caggatatgg catacaggtt
     6121 ggtttacgcg ttgaatttta ataacgtcag gtgaagtcgg gctaaataat taaccggaaa
     6181 agtagttaag aaaattgctt taaattaaac ttctgccact acttcagctt cttcaattac
     6241 atctttttct acacgaaggt ttttaataat aaatccctgg cgttcaggtg tgttttttcc
     6301 catgtaatat tctaaggtgc tttgaatggt ttgttctttt ccgataatta ccggctcgag
     6361 acgaatgctt tcaccaataa atccgctgaa ctcatcagga gaaatttcac ccaaaccttt
     6421 gaagcgggta atttctggct tgttgcctaa ttggttaact gcattttgtt tttccgtttc
     6481 agaataacaa taaatggtag attttttatt gcgcacacgg aataatggtg tctgcaaaat
     6541 atatacgtga ccatttttta ccaaatcagg gaaaaactgt aagaagaaag tgagtagtaa
     6601 taatcgtata tgcataccat ccacatcggc atctgtggct attacaattt ggttataacg
     6661 caatccttca ataccatctt caatattaag tgcatgttgc aataaattaa attcctcatt
     6721 ttcatacaca atttttttgg taagtccgta cacatttagt ggcttacctt ttaacgaaaa
     6781 cactgcttgt gtttctacat cacgggcttt tgtaatacta ccgctcgcgc tatccccttc
     6841 tgtaataaat aaggttgagt taatagtttt ttcgtgttta tcctctccta agtgaaagcg
     6901 gcaatcgcgc aattttttat tgtgcaagtt cgctttttta gcgcgttcgt tggctagttt
     6961 tttaataccg gcaatatcct tgcgttcgcg ttcgctttgt aatattcttt tgagcaaagc
     7021 atccgcagtg gccgtatttt tatggagata attatccaat tcttttttga taaaatcaac
     7081 cacaaaactt ttaacggtaa caccatccgg cgacatattt attgagccga gttttgtttt
     7141 ggtttgcgat tcaaatacag gttcctgcac acgaatggct atagcgccaa caatagcctg
     7201 acggatatcg gaagcatcaa aatctttttt ataaaattcc cttaccgttt tcacaatacc
     7261 ttcgcggaaa gcggctaaat gtgttccgcc ctgtgtcgta aactgaccgt tgacaaacga
     7321 ataatattct tcaccatatt cattattatg ggtgagcgac aattcaatat cgtccccttt
     7381 taagtgaata attggataac gcaaatcatc ttcattggtt ttgcgtttta ataaatcgag
     7441 taatccgttt tgagagtaat atttctgacc gttataatga atggttaagc cggcattcaa
     7501 aaaaacataa ttccacaact gattatcgag gtattccgga ataaaatgga agtttttaaa
     7561 aatcgtattg tccggaataa atgttactaa tgtgccatcc ttttggtcag atttaattat
     7621 cgcattgtct tttcgtaaaa tccctttttc aaattccgcc attttcgttt ttccttcgcg
     7681 gaaggcttca acacgaaaat aatggctaag tgcgttaacg gctttagtac caacaccatt
     7741 taacccaacc gatttttgga atgcagcgga atcgtatttg gcaccggtgt tcatcacgct
     7801 aacacattct atcacttttc ccagcggaat accacgtccg taatcgcgaa cggtaacttt
     7861 ataatcctca atttctactt caatcttttt gccgtaaccc atcatgtatt cgtcgatggc
     7921 gttatcgata acctctttta caaggatata aataccgtca tcaggcgaag aaccatctcc
     7981 gagtttacca atatacatcc ccggacgcat gcgcatgtgc tcaatccagt cgagggtcct
     8041 gatatgatct tccgaataat tataattggt agtagccatg gggcgaaaat aggagatttg
     8101 ggcagcatat atcaaagatg atatgcacat cctgcggttt gtacgtagta aaacctcaat
     8161 agccttagca gcttcttatc gaagacttgc ctgtcaaact tactaaaagt caaatccatc
     8221 caactctaca cccgcaaggg tgtaagggtg taagggtgaa taattattca ccccgacgtg
     8281 aagtataatt gtaattgtca cacacctccc aatcacaaca actcattcgc caaattagcg
     8341 agctcactgc gctctccttt ttgaagtgta atgacagaac acataggatg gttattgatt
     8401 ttatcaatca gataacttag tccgttgcta tgcgtatcta ggtatggtgt atcaatttga
     8461 ttgatgtcgc ctgtgaaaat aattttggtg ttctctcctg ctctggtaat aatcgttttt
     8521 acttccagtg gtgtgagatt ttgtgcttcg tcaatgataa aataaatatt agaaatactt
     8581 cttccacgaa tatatgccaa aggttgaata acgagttttt cctgttcaat catttgcgca
     8641 atagccttgt attcgctgtc gttttgttcg aattgattct gaatcatttt taaattatcc
     8701 caaagtggtt ccatgtatgg attcaatttg gatttaatat caccgggtaa atatccgatg
     8761 tctttattat tcagcggaat aattggtctt gctaaaaata tttgtttgta attgcggcgt
     8821 tgttccagtg cagaagccaa tgccaataag gtttttccgg ttcctgactg tccctgcaag
     8881 gctaccagtt tgatatccgg gtctaataat acatttaatg cgaaaacctg ttcagcattg
     8941 cgcggtttaa tgccgtaggc agttgcttta ttgatacgtt caaaaacttt ttcttctgaa
     9001 ttataacttg ctaaaacgct gctgcttcca ttttttaaaa taaaacactg attggcaaac
     9061 ggttcttgtt ttaatatttt tttaaatccg ataaatccgt cgctataaat tttgtcaata
     9121 gattcatggt caataccttc aatcgtttct attggctctt ttaataaatc ggtgttttca
     9181 attttatcat ttttataatc ttcactatat aaaccaagcg atttagcttt taatcgcagg
     9241 ttgatatctt tcgtcaccaa aataacctta tgctcaggat atttttgttt cataaccaaa
     9301 gccgtattca gaattttgtg gtcgggtttt tcttccccaa acacctcttc cgcattgagg
     9361 cctgtatgcg cgtacatggc taccagcata cgtcctgtgg ttcgtccgtt tatcggtcgc
     9421 cactcttgta gagtgctttc cttcgactta ttatcaagaa agcgaataaa ttctctggct
     9481 tcgaaattga tggtttcgtt gcccttttta aaattgtcta attcttccag cactgtgatg
     9541 ggaattacaa cgtcgtgttc ttcgaattta tttaccgaat tgtggtcgtg cagaataacc
     9601 gatgtgtcga gtatgaaaat ttttttctct tcctgcactt tagccttttt cttcaatgcc
     9661 atatcgtggt ttgttatcca agattagcgg atgtacacaa taaaacaaac agggttatac
     9721 cacatcaatg ttgataattg tctgtacaag cgtacagctc aggctggaag tgcctgtaca
     9781 agcgcagttt caaaaaaggc taattgttgc gcagcctcca cttcatctaa acttaatttt
     9841 gcagcgataa tttttgctat ttcaggatat aattgtttgg tctgttccat atcaaaatat
     9901 aaacgaccgg ttctgcgaat tataaaatcg ctgacattag taaccatttc atgttctatt
     9961 gcatacataa tttcggctgt gagcaatcgt gatgcgggat ttttaattgc attaccgata
    10021 tcaaatgcat agttaattat cgtttcactg cctgtgccgt aagtatacac caaataggaa
    10081 attacatttt catctacatc aatctgcttg gcttcacctg ttctgcgtgt gatgtattcg
    10141 ggtatactgc aatcaaaatc agcgccaaat aataataact catgtgtttt gctgttagga
    10201 atttttctac ctgatttttt aaatgatttc gctaacatat ttaccacttt ctccgccatt
    10261 tttcggtagc cggtcaattt acctccggct atagatatca gccctttatc agaaataaat
    10321 atttcatctt tccgtgataa ttcagaaggc gatttacctt cttcatgaat aagtggccgc
    10381 aaacctgccc agcctgaaat tacctgcgaa tattgcaact gcactttggg aaatttatta
    10441 tttacggcat ccaataaata attaacatcc tctctggaaa gtaaaatatt atccttatcg
    10501 ccaacataat tggtatcggt tgtgccaatg taaacggatt ttccgcgagg tattacaaaa
    10561 atcattcggt tatcgcctgc aacatcaaaa tataatgcat gctgaacagg aaaatcgttt
    10621 ttgtttacta cgatatgtat gcctttggtg agatgtaatt tttttccttt aggtaatgtt
    10681 tccagactcc ttacatcatc tacccaaggc cctgcagcat taattacatg agttgcatta
    10741 aatgttatag ttgtatcgga taataaatcg cgtgcaacaa caccatttat aatattattg
    10801 gtaaaaatga aattttccac tttcatataa ttaaaacaac gggcgccaaa agttgcggca
    10861 gatttaatta cacttattgt taatcgtgca tcatcagttc taaattcttt ataaatagca
    10921 ccaccaatta agtcccgggt cgataataat ggttccagtt gatgtaaatg tgttggatcc
    10981 accatataat atctatcgtc tcgttccact tttgctaaag tatcataaat gcgtagtgca
    11041 attcccgtag aaaattttcc aaggctgcca tttttgtaaa tcggcaaaac catattaatc
    11101 ggttcaacca aatgtggagc aagtttatgg ataacagccc gttcaatccc caccgtacgc
    11161 actaacccaa attccaattg ttttaaatac cgtaatccgc catgtatcag ctttgtagat
    11221 ttagatgaag tccctcccgc gaagtcgtac ttttccagta acaacacttt taatcctctt
    11281 gaagcagcat ccagcgcaat tcctgcgccc gtaatacctc caccgatgat gatgacatca
    11341 aattgggtat ttgctgcagc atgcatattt gctgctcggg ttaaggtgtt tagtggcata
    11401 agtatatatg caaatgtgca aaaaacaatt tgttttaaat attttagtgt tcaattattc
    11461 taattaactt tataaaaaca ctacactcat gagcaaaatc aggattatct gtttatgtat
    11521 acttttttta ggagtaaata agatttatac ccaagtcccg actttagact gggcgggaaa
    11581 aattggtaaa aatggctcag attatggcta tcaggtccat gccggtcttg acggcaacat
    11641 ttatgctgtt ggtacattta agggcaataa tgttgatttt aatatcgata ctgcagctgc
    11701 agaggttgat gtattttcct ccggtaatta taatggttcc attttagtta aatacaccaa
    11761 aacgggtgaa tttttaggtg gattctcatt acattctgat aatgatatgg ttattaatgg
    11821 cacggtaacc tttaatagta tttatgttga aagtgacggc gaaatatatg tcacgggttt
    11881 ttatcagggc accaccgatt ttgacccggg tgttggcgaa aatattttaa ctgcaattgg
    11941 agatgcagat atttttattg ccaaatattc gggtgatttt actttacaat gggtgaaaag
    12001 aataggatat gccggtcgat tgtccggtgg cgaaaaattg gtattaggca atgatggtgc
    12061 tatttatgta acaggttatt ttggtggtgt ttgcgatttt aatcctgatg atattgccgt
    12121 caacaattta accagtttgg gtacagatga tgcttttatt gct
//
LOCUS       DAORKW010000044        12135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_90_length_12135_cov_5.156209, whole genome shotgun sequence.
ACCESSION   DAORKW010000044 DAORKW010000000
VERSION     DAORKW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90_length_12135_cov_5.156209"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="PLB46_03320"
     CDS             complement(<1..1641)
                     /locus_tag="PLB46_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRF75575.1"
                     /translation="MMKKWMRCLFLVMSLTGLQQAHAQPDNSGKKNNFSGNNYSSYLA
                     HIAAANASIRLHDGAEAQRWLTSTPVEYRGWEYNYLKNTIQGYTHSFNLLYEANEIDL
                     SNDGKKMAIAGADSLIHICSADGLVEQKTYKGHTNTVYAVDYFGNDTKLVSCARDFTI
                     RVWDTQNDIQLWKKTTKAQGICDVVGSPDGKLVALSSWYRDEDHNHVVGFIEVYDAFT
                     GELKWETTFGEKPLVAITFSHDGKLLAAAGWNWAVAVWDVQTKEKKHAFNFDDVTTYS
                     AIDDLVFSADDAYLMATSRNTTARVWNMYTGTIQFELKGHENAVYAGDFTNDGTLMIT
                     GSADGSLILWSTETGLPVNSLFGHQSVITDITMAPDGNTFYTLANDKTIKTWSLKNAA
                     PYSNPSGQNGLVYGFNISKDNQYLVTQGDNGILTVWNNNDGSIVKQFPTFGGIMNAAA
                     ISPDNNYVVGCNWEGRIKVWELFTGKKAIEFAGINAGVVGCTFSPDGKQVAVAAYDST
                     ITIWDLASGARLHQLKHSGLMYNVTYSPNGKYIVGSSTN"
     gene            complement(1644..2021)
                     /locus_tag="PLB46_03325"
     CDS             complement(1644..2021)
                     /locus_tag="PLB46_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75576.1"
                     /translation="MKGIIVITLVALSLHLHAQDSTKSAYLGISTLQPFYQQDSNWTD
                     ADDAIIGAHFVRLQQLAKEGKIELAGRVNLPSNDPNMFGIVLILPTTLAEAEKIMLED
                     PAVTGKIMSAKILPFNIAVQKSD"
     gene            complement(2039..2440)
                     /locus_tag="PLB46_03330"
     CDS             complement(2039..2440)
                     /locus_tag="PLB46_03330"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HRF75577.1"
                     /translation="MEKLNVIAVIVAAVSMFLIGGLWYSKALFGNVWMRENNLTEEQL
                     AKSNKGKTFGLSFLFSLIMSFNLAMFVSDPSIDFTMTIFYALCVGLGWIALAFGIVAL
                     FELRSWKYIFINGGYMVISFLVMGIILGAWK"
     gene            2511..3593
                     /locus_tag="PLB46_03335"
     CDS             2511..3593
                     /locus_tag="PLB46_03335"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF75578.1"
                     /translation="MDTLLPKPENSLLKPENKVPAYLNDKGLRMVLIPLFAIAVPNLT
                     GLVSYHFNWWTLILHYCYYLFISIAIYYGNRYLLFQLSDRFNWFYSPFRKVLTLLAGV
                     ILYTLPVTLFLIGLYYICILQQPIDEVVLRNVSLMNVICVIFVAHIYETVFLIKHTEG
                     EMLRIAQVEKAKAEAELEALKAQIDPHFIFNSLNTLSHLITQNPEKAKKFNDNLADVY
                     RYILQHKNKSLVFLRDEMSFMENYYALLHIRFGESLQLQNEVHPSQLEHYLLPPISLQ
                     IILENCVKHNAFSEIQPMPIRIFIEENMLVVRNRLMPKKTIKQNTGTGLVNLKGRYRL
                     LAENEIVINQHEDHFEVRLPLLSTGI"
     gene            3590..4345
                     /locus_tag="PLB46_03340"
     CDS             3590..4345
                     /locus_tag="PLB46_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF75579.1"
                     /translation="MNIIIIEDEKPALEKLRLQLNNYQYPVNILVTLSGAQESVEWLN
                     ENAHPDLIFCDIALSDGISFDIFRQVKVKCPVIFITAYDDYLLEAFELNSIDYLLKPV
                     SQERLFVALNKYFQLKEHFASNYSGLADAYFAGQHQKTRYLVKKGVSYSTVKLEDIAY
                     FISEHKITFLIDKSGNKYILDKTLQELETELPKNDFFRINRKYIGNISAITKFKPFTK
                     GKILVELTPAVGEEIIVSAENAAAFKMWVSGDS"
     gene            complement(4447..6918)
                     /locus_tag="PLB46_03345"
     CDS             complement(4447..6918)
                     /locus_tag="PLB46_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF75580.1"
                     /translation="MKRILVLLYCISLLQLAFSQGQMHAKKNDYVSITNGTTKIRYPE
                     ISEELLNATPESYYANPDFGILPVNAPDNAIELIQKRTPTSRYYIKPNTAGNKFFIQQ
                     AYGPINYFDPSGHLRAIDFYLHPTLVKGIYEAEAQPAPTKIDFVNNFTAIRIKDLDYR
                     YNADLQLFFQTGNNFTTPQKAMLTNHTAGRDGVWITDAFEKVDMEIAFRQNAIKTNFI
                     IKDKSIVNPNSEYLIIDDPISLPEGYFLEMEDVDGYFLDNGDWKGDLILKNQFGLPYL
                     RIEKPLVLDQSDEKTHAMDQVNAVAYQLIKTATGYTIRLRVSTKWLLAPERIYPVIVD
                     PTLIGEATYTAEDIGFEFDVLCWNEADYCNYFLDVTVPGKTTLTAAYFDGTYYSQNFG
                     CFFVTDCLMKEAAFRILGICDDSPSAGGFWTCSPPAGDTAGTCYGIDLDMFNTIACIP
                     PQCDDYEFTFEMRTWHCSCTQPPCGILCHFMPAGSWVITIEGETVVENPIQSSTYPDF
                     VICEGDTIDLFANGYWGVPPYTYEWLPGGTVEPIHEVWPTTTSTYTSIIHDACDMTDT
                     VTQTVTVNAAPVVNIGPFEACYETTITAPAGYDNYVWTDADSNFLASGNTLYVDSTGE
                     YFVTVIDANGCEGTSSPILVNIYEAPIINAIPDTVFVNNGALALLEAEIIEGDDVGFL
                     WVPESYANCPTCPSTLAYTEGEEFVFYVYGVENGCVSLPDSIIVVMQESELIIPNAFT
                     PNDDGLNDEFNILNPIFFPEFSFRIFNRWGQEVFATSDVLKGWDGTYDSINQEIGMYV
                     WMVIYEKANEPGKQYILRGTVTLLR"
     gene            complement(6982..9900)
                     /locus_tag="PLB46_03350"
     CDS             complement(6982..9900)
                     /locus_tag="PLB46_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF75581.1"
                     /translation="MMKFYTTLLCFLAAFSLGAQTFQLFTEDFNVPSGSFTLNTAGPD
                     GVATGTNKWIINDSYTGGFGYANTTSQELTETGTIGGAPYSPYLHIYDEEGAPAITCA
                     SYDATDPSDNFTEMSGGFCTLGLIDVEFTFFWLCEGGAGDYGQVYYSADGGPWTPVGM
                     GLYSGQTLWKYEVITDPAFEDVANLRFGFRWINNADGGANTMSFAIDDIIAVGTYDEA
                     VHPVDINIDFVIPDPVCKLSTLIIGWSLSDPLCTGIYEIELSNAAGAFAAPTSLGVFT
                     ILAEDTVGAIAATIPGAIPDGSCYKVRINRTSPLPEISGEASVCFVVENCPNVITTLK
                     PVITIDTNAICVNSVIDVPFYSTGVYNPGNIYTAQLSDSTGSFDTFSVIGTLNSNATY
                     DPALGSPPGSVSGIVPMVPPGCNYLIRVVSSNPVATGSPYGTVCIQECDIETNDIEDV
                     YACITEDSGVTITITYDVNVFDEVETYCDTNTFCVEVLNSMFFTQANLCGLGFTVDTE
                     SGTIDLEIPGYFDLLAMGLDAGMWYMRVIANCGTPQENTLGTLIHLTIGAPADNAPIL
                     IPADTLLCEGAIGSATVVPYNPNSDYQFQFGLGTPFVWPFNPIFIDFTGLPGDYTLRV
                     REINYNCPGPWSEYITFHIIDVPIVTITGPIKACTGDTVYYSVPYFLTTYYDWSLSGG
                     AIVDTANNVVGIVWDEAGTYTLNVFALNECGSGNGSKNITIIETIPVENTDDVTICEG
                     ETATFSVLTVGVPYYEWSDETGTLISDDFYLTVTPDSSSTYYLTAEDDEGCPSWDTIS
                     VFVEYPEFEFDTTEYCIGGSVILDAGYPGATYVWNTGATTQTIEVSLFGTYTVQIDNP
                     DQACDITKTVYVNEVIDNCDPIINIPNAFSPNGDGFNDNLVIVGAAIVDIEIIIYNRW
                     GEIVFQSDDVGIVNNPAFGWDGRVNDVDQEMGTYVYQLSATGGSGTTVQLQGNITLVR
                     "
     gene            10063..11538
                     /locus_tag="PLB46_03355"
     CDS             10063..11538
                     /locus_tag="PLB46_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRF75582.1"
                     /translation="MSLRRLANVTAIYGLSTILVRVLNYLLAPLHTRVFTDQSDYGII
                     SEMYAYVTFINVICMFGLETAFFRFASNSTNSLEKKTIFGTAQFSLIVSTILIVGTII
                     FSAGNIAELLDYPGNANYIIFFALIIGFDTLVNIPFAKLRLEGRPWRYFSIKLINIGI
                     NILLNLFFLWPALKNNYTLFEPFGFTYQPQYGIAYVFIANLIASGVTFLLFAPGMFNI
                     RFDLPTWKKLMQYGAPLIIIGLAGMINETLDRVLIKYWGSGTMEENLKQVGIYSAVYK
                     LSIFMTLAVQGFRMGAEPFFFSQSADKNAPSLYALIMKYFVVVCCVIFLGVGLFPDLF
                     KIIIGEEYHEGLAIVPILLLANLFLGIYYNQSVWYKLTDKTVFATIIPLTGAALTLLF
                     NFLLIPQLGYTGAAWTTLICYFSMVVMSHIIGQKHYHVPYNLRKIIAYIIISVVLCLI
                     GLWLNKIISNQLISIIIRAIFMGVFFVFAWWLDLHKLLRKT"
     gene            11600..12031
                     /gene="dut"
                     /locus_tag="PLB46_03360"
     CDS             11600..12031
                     /gene="dut"
                     /locus_tag="PLB46_03360"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRF75583.1"
                     /translation="MEIKIINKSNNPNPSYATAQSAGMDLRANLETEMVLLPGQRMLI
                     PTGIFIELPIGYEAQIRPRSGLAINSGITMLNSPGTIDADYRGEIKVIAINHSDQPFH
                     INNGDKIAQMVVAKHEFVSWINVANLEKSERGDGGFGSTGG"
BASE COUNT         3852 a         2355 c         2132 g         3796 t
ORIGIN      
        1 atttgtggat gagccaacaa tatattttcc attaggagaa tacgtaacgt tatacatgag
       61 tccgctatgc tttagttgat gtaagcgcgc acctgatgct aagtcccaaa ttgtaatagt
      121 actatcataa gcagcaacag caacttgttt accatcgggg ctaaaagtac atccaacaac
      181 tcctgcattt attcctgcaa attcgatagc tttttttccg gtgaataatt cccacacttt
      241 aattcgtcct tcccaattac aacctactac ataattatta tccggactaa ttgcagctgc
      301 attcattatt ccaccaaaag tgggaaattg tttaacaata ctgccatcat tattattcca
      361 aacagttaat attccgttgt caccttgtgt taccagatat tgattatctt ttgaaatatt
      421 aaaaccgtaa acaagaccat tttgcccaga gggattgcta taaggagcag catttttcag
      481 gctccaagtt ttaattgttt tatcattagc gagtgtataa aatgtattgc catccggtgc
      541 cattgtgata tcagtaatta cactctgatg tccgaataaa ctattaacag gcaaacctgt
      601 ttcggtactc cataaaatta aactaccatc tgcacttccg gttatcatta atgttccatc
      661 attagtaaaa tcgccggcat acacggcatt ttcatgacct tttaattcga actgtatggt
      721 gcccgtatac atattccata cacgcgcagt ggtgtttcta ctggtagcca ttaagtaggc
      781 atcatcggca gaaaaaacga gatcatctat cgccgaataa gtagttacat catcaaaatt
      841 aaatgcatgt tttttttctt ttgtttgaac atcccaaacc gcaacggccc aattccatcc
      901 tgctgcagcc aacaatttgc catcatgact aaatgttatc gccaccaaag gtttttcacc
      961 aaaggtggtt tcccatttta attctccggt aaaagcatca tatacttcaa taaatccgac
     1021 aacgtgatta tggtcttcat cgcggtacca tgagctgagt gcaactaact ttccgtcagg
     1081 gctgccaaca acatcacaaa taccttgagc tttagttgtt tttttccaaa gttgaatgtc
     1141 attttgtgta tcccaaacac gaatggtaaa atcacgtgcg caggaaacta atttagtatc
     1201 gttgccaaaa taatcaactg catatacagt attagtatgg cctttatagg ttttttgttc
     1261 taccaatcca tccgcagagc aaatatgaat gagactatct gcgccggcaa ttgccatttt
     1321 ttttccgtcg ttactcaggt caatctcatt agcttcatat aacaaattga aggaatgtgt
     1381 atatccctga atagtatttt tcaaataatt atattcccat cctctatatt ctaccggagt
     1441 ggaggttaac caacgttgag cttcggcacc atcatgcaac cgaatagaag catttgctgc
     1501 agcaatatgc gcaaggtaac tggaataatt attaccggag aaattgtttt ttttaccgct
     1561 attatcaggt tgtgcatgag cttgttgaag tccggttaaa ctcattacca aaaacaaaca
     1621 acgcatccat tttttcatca ttattagtcc gatttttgta ctgcaatatt aaaaggtaaa
     1681 attttagcag acataatttt gcctgttaca gccgggtcct ccaacattat tttttcggcc
     1741 tcagctaatg ttgtcggcaa aattaaaact attccaaaca tgttggggtc atttgagggt
     1801 agatttaccc tgccggctaa ttctatttta ccttcttttg ccaattgctg taatcggaca
     1861 aaatgtgcgc caataattgc atcatcagca tcggtccaat tgctgtcctg ctgataaaac
     1921 ggctgtaaag ttgatattcc aaggtaggca gatttggtgc tgtcctgtgc atgcaaatgc
     1981 aacgacagtg ccacaagcgt aataactatt atacctttca ttttttaaaa ctaaataatt
     2041 acttccatgc acccaaaatt attcccataa ctaaaaaaga aataaccatg taaccgccat
     2101 ttataaaaat atatttccag ctgcgtaatt caaacaatgc aacaatacca aaggcaaggg
     2161 ctatccatcc taacccaacg cacaatgcgt aaaaaatagt catagtaaaa tcaattgatg
     2221 ggtctgaaac aaacatggcc agattaaacg acatgatcag cgaaaataaa aatgacaatc
     2281 caaaggtttt gcctttattg gatttagcca gctgttcttc ggttaaatta ttttcacgca
     2341 tccaaacatt gccaaaaagc gcttttgaat accataatcc accaattaaa aacattgaaa
     2401 cagcagcaac tattactgca ataacattta atttttccat aaatagtatt tttgttgtaa
     2461 attcgaaaat aggttaccca tttaatttaa aataatattt accctttaaa gtggatactt
     2521 tattacccaa accagaaaat agtttactga aaccggaaaa taaggttccg gcatatctca
     2581 acgacaaagg tttgagaatg gtattaatac cattgtttgc tattgctgta cctaatttaa
     2641 ccggtttggt aagttatcat tttaattggt ggacgcttat tttacattat tgttactatt
     2701 tatttatttc cattgccatt tattacggca acaggtattt attatttcag ttaagtgata
     2761 gatttaattg gttttattca cctttcagaa aggttttaac attactagcc ggtgtaatat
     2821 tatatacatt gccggtaacg ttatttttaa taggcttgta ttatatctgt attttgcagc
     2881 aacccattga tgaagtagta ttacgcaatg taagtttgat gaatgtaata tgtgtaatat
     2941 ttgtagctca catttatgaa actgtatttt tgattaaaca taccgaagga gaaatgctga
     3001 ggattgcgca ggtagaaaaa gcaaaagcgg aagctgaact agaagcgctt aaagcacaaa
     3061 tagacccgca ctttattttt aattctttaa ataccttatc tcacctgatt acacaaaatc
     3121 ccgaaaaagc gaaaaaattt aatgataacc ttgccgacgt ttacaggtat attctgcaac
     3181 ataaaaacaa aagcttggta ttcctgcggg atgaaatgtc ttttatggaa aattattatg
     3241 ctttactcca tattcgtttc ggtgaaagtt tgcagttgca gaatgaagtt cacccatctc
     3301 aattagaaca ttatttgttg ccacccattt cgctgcagat aattttagaa aattgcgtta
     3361 aacataatgc cttcagcgaa atacaaccca tgccaattcg cattttcatt gaggagaata
     3421 tgctggtggt gagaaacaga ttgatgccca aaaaaacgat aaaacaaaat acaggaaccg
     3481 ggttagttaa tttaaaaggg cgttacaggt tattggcaga aaatgaaatt gttattaatc
     3541 agcacgagga ccattttgaa gttcggttgc ccttattaag tacaggcata tgaatataat
     3601 aataatcgaa gacgaaaaac cggcattgga aaagttgcgc cttcaactta acaattatca
     3661 gtatccggtt aatatacttg ttactttatc cggggctcag gaaagtgttg agtggttaaa
     3721 tgaaaacgcc catcccgacc ttattttttg tgatattgca ttaagcgatg gtatttcatt
     3781 tgatattttc agacaggtaa aggtgaaatg tccggtaata tttataaccg cttatgacga
     3841 ttatttgctg gaagcttttg agctcaatag tatcgactat ttattaaagc cggttagtca
     3901 ggagcggttg tttgttgctt tgaataaata ctttcagcta aaggaacatt ttgcaagcaa
     3961 ttattcggga ttagcggatg catattttgc tggacagcat caaaaaacgc gttatttggt
     4021 gaaaaaaggt gtgtcttatt ctactgtgaa actggaagac attgcatatt ttatttctga
     4081 acataagata acgtttttaa tagataaatc gggcaataaa tatattttgg acaaaacttt
     4141 acaggaatta gaaaccgaat taccaaaaaa tgatttcttc agaattaatc gaaaatatat
     4201 tggcaacata agtgctatta caaaatttaa accttttacc aaagggaaaa tactggttga
     4261 attaacgccg gctgttggag aagaaataat tgtaagtgcg gagaatgctg cagctttcaa
     4321 aatgtgggtt tcgggtgata gttaaaatgg tgctttaaca gccgaaagca cgaagcgaca
     4381 aagtagtgct aaggtacatc aagatgatgt tgaataaata aatttggtag cagtaaacat
     4441 ttaaccttac cgcaacaagg taactgtacc ccgaagaata tattgtttgc cgggctcgtt
     4501 ggctttttcg tagataacca tccaaacata catgccaatt tcctgattaa tactatcgta
     4561 tgtaccatcc catcctttta atacatcgct ggtggcaaat acttcttgtc cccaacggtt
     4621 aaaaatccgg aaagaaaatt cgggaaaaaa tattggattg aggatattaa attcatcatt
     4681 taatccatca tcgtttggtg taaatgcatt tggaataatt aattcacttt cctgcataac
     4741 tacaatgata gaatcgggca atgaaacaca tccattttca acgccataaa cataaaatac
     4801 aaactcttct ccttcggtgt atgctaatgt gcttggacaa gttggacagt tagcataaga
     4861 ttccggcacc cacaaaaatc caacgtcatc accttcaata atttcagctt ccagcagagc
     4921 taatgcacca ttgtttacaa aaaccgtatc aggaatggca ttaattatag gggcttcata
     4981 aatattgact aaaattggtg acgaagttcc ttcacatccg ttggcatcaa ttacggttac
     5041 aaaatattca cctgttgaat caacatacaa ggtgtttccg cttgctaaaa aattggaatc
     5101 tgcatctgtc caaacataat tatcgtaccc ggcaggtgca gtaatggtgg tttcgtaaca
     5161 agcttcaaaa gggcctatat ttacaacagg tgctgcatta acagttaccg tttgggtaac
     5221 cgtatcagtc atatcacatg catcatgaat aatggatgta tatgttgaag tagttgttgg
     5281 ccaaacttcg tgaatgggtt caacggttcc tccaggcaac cattcgtaag tatatggcgg
     5341 aacaccccaa taaccattag caaataaatc gatagtatca ccttcacaaa taacaaaatc
     5401 gggataagta gaagattgaa ttggattttc tacaacagtt tctccttcta ttgtaattac
     5461 ccaacttcct gctggcataa aatgacataa aataccacaa ggtggttgtg tgcatgagca
     5521 gtgccatgtg cgcatttcaa aagtaaattc ataatcatca cattgcggtg gaatacatgc
     5581 aatggtatta aacatatcga ggtcaatacc atagcaagta cctgctgtat cgccggcagg
     5641 cggactacaa gtccaaaaac cacctgcaga aggcgaatca tcacaaatac ccagaatacg
     5701 aaaagcggct tctttcatca aacaatcggt aacaaaaaaa catccgaaat tttgggaata
     5761 ataagtgcca tcaaaatatg cagcagttaa tgttgtttta ccgggaacag ttacatctaa
     5821 aaaataatta caataatctg cttcattcca gcacaataca tcaaattcaa atcctatatc
     5881 ttctgctgta tatgttgctt cgccaattaa ggtagggtca acaattaccg gataaattct
     5941 ttcaggcgcc aataaccatt ttgtgcttac gcgtaaacga atagtatatc ctgttgcagt
     6001 tttaattaat tgataagcaa ctgcatttac ctgatccata gcatgcgttt tttcatcact
     6061 ttggtctaac accagtggtt tctcgatacg taaataaggc aaaccaaatt gattttttaa
     6121 aattaaatct cccttccaat caccattatc taaaaaataa ccatctacat cttccatctc
     6181 caaaaaatat ccctcaggca aacttatagg gtcatcaatt atgagatatt cactattggg
     6241 attaacgata cttttatctt tgataataaa atttgttttg attgcatttt gtctgaatgc
     6301 aatttccatg tctacttttt caaatgcatc tgtgatccat acaccatcgc gaccggcagt
     6361 atgattagta agcatggctt tttgtggtgt ggtgaaatta tttccggttt gaaaaaataa
     6421 ttgcaaatct gcattgtatc ggtagtcaag gtctttaatt cggatagccg taaaattatt
     6481 tacaaaatca atttttgttg gtgcgggttg cgcctccgct tcataaatgc cttttaccag
     6541 tgttggatgt aaataaaaat caattgcgcg caaatgtcct gaagggtcaa aataattgat
     6601 tggcccatag gcttgttgaa taaaaaattt attgcctgcc gtattgggtt taatataata
     6661 cctggaagtt ggtgtacgtt tttggattaa ttcaattgca ttatcaggag cattcaccgg
     6721 taaaatacca aagtcgggat tggcataata agattccggg gttgcgttta ataattcttc
     6781 actgatttcc ggataacgaa tttttgttgt accgtttgta attgaaacat agtcgttctt
     6841 ttttgcatgc atttgccctt gcgaaaatgc aagctgcaat aaagagatgc aataaagcaa
     6901 tactaatatt cttttcataa tatgttaccc ggcagcatgc cagcactcat ttcaattgat
     6961 tgaaatttac cttttcctta tttatctcac caatgtaata ttaccttgca actgcacagt
     7021 tgtgccactg ccaccagtag cacttaattg atatacgtaa gtgcccattt cctggtctac
     7081 atcatttact cttccatccc aaccaaatgc cggattattt acaataccta catcatcact
     7141 ttggaaaaca atttctcccc aacgattata aataataatc tctatatcaa caattgcagc
     7201 accaacaatt accaggttgt cgttgaagcc atctccattc ggtgaaaacg catttggtat
     7261 attaataatt gggtcacaat tatcaatcac ttcattaacg taaactgttt ttgttatatc
     7321 acaagcctga tcagggttat caatctgcac tgtgtatgtg ccaaataatg atacctcaat
     7381 agtttgcgta gttgcacctg tattccaaac atacgttgca ccaggataac ccgcatctaa
     7441 aattacgctt ccaccaatgc aatattcggt agtatcaaat tcgaattcag gatattcgac
     7501 aaatacgcta atggtgtccc aacttgggca accttcatcg tcttctgctg ttaaataata
     7561 agttgatgat gaatctggtg ttactgttaa ataaaaatca tcgcttatta atgtaccagt
     7621 ttcatccgac cactcgtaat atggcacacc cacagttaat acactaaatg ttgctgtttc
     7681 accttcacaa atagttacat cgtcagtatt ttctaccgga atagtttcta ttatggtaat
     7741 atttttagaa ccgtttccac tgccacattc atttaatgca aatacattta aagtatatgt
     7801 tccggcttca tcccaaacaa taccaacaac attatttgct gtatcaacaa tagcaccacc
     7861 cgataaagac cagtcgtaat aggtggttaa aaaataaggc actgaataat agaccgtatc
     7921 accggtacaa gctttaattg gtcctgtaat agttactatt ggtacatcaa taatgtggaa
     7981 tgtaatatat tcactccaag gccccgggca attataattt atttcgcgaa cgcgtaaagt
     8041 atagtcgccg ggtaaaccgg taaagtcgat aaatattgga ttaaacggcc atacaaatgg
     8101 cgtccctaaa ccaaactgga attgataatc tgaatttgga ttatacggca ctactgttgc
     8161 acttccaatt gcaccttcac ataatagtgt atctgccgga attaaaatcg gtgcattatc
     8221 tgcaggagct ccaatggtta aatggattaa tgttcccaag gtattttcct gtggagttcc
     8281 acaatttgca attacacgca tataccacat tcctgcatca aggcccattg ccaataaatc
     8341 gaaataaccc ggaatttcta aatcaattgt ccccgattct gtgtcaactg taaatcctaa
     8401 tccgcataaa ttcgcttgtg taaaaaacat agaatttaat acttcaacac aaaatgtatt
     8461 cgtatcacaa tatgtttcta cttcatcaaa aacatttaca tcataggtaa tggtgatggt
     8521 aacaccggaa tcttcggtaa tacatgcata tacatcttca atatcatttg tttcgatatc
     8581 acattcctga atacaaactg ttccatacgg tgaacctgtt gcaaccggat tgctggaaac
     8641 aactctaatt aaatagttac atcccggagg aaccatcggc acgataccgg atactgaacc
     8701 cggaggtgaa cccaatgcag gatcataagt agcattacta tttaaagttc ctattacaga
     8761 aaatgtatca aaacttcctg ttgaatcgct caattgtgct gtgtaaatat ttccgggatt
     8821 ataaacgcct gtggaataaa acggcacatc gataacacta tttacacaaa tggcattggt
     8881 atcaattgtg ataacaggct ttaatgtagt aattacattc ggacaatttt ctacaacaaa
     8941 acagacgctc gcttcaccac tgatttcggg taaaggtgac gttcggttga tgcgcacttt
     9001 ataacacgac ccatctggaa tggcaccggg aattgtcgca gcaattgcac caactgtatc
     9061 ttctgcaaga atagtaaata cacccaagct ggttggagct gcaaaagcgc cggctgcgtt
     9121 ggaaagttca atttcataaa tacctgtaca aagcggatca gaaagcgacc agccaataat
     9181 gagcgtgctg agtttacaaa cagggtcagg aattacaaaa tcgatattaa tatcaaccgg
     9241 atgaacagcc tcatcgtaag taccaacagc aataatatca tcaatagcaa acgacatggt
     9301 gtttgcgccg ccatcggcat tatttatcca tcgaaaacca aaccgcaaat tggccacatc
     9361 ttcaaaagcc gggtcggtga ttacctcata tttccagagg gtttgaccgc tgtaaagtcc
     9421 cattccaacc ggtgtccaag gtcccccatc ggcactgtaa tatacttgtc cgtaatcgcc
     9481 agcaccacct tcacacaacc aaaaaaaagt aaattcaaca tctatcaaac ctagtgtaca
     9541 aaagccaccg gacatttcgg taaaattatc ggaagggtca gtcgcgtcgt agcttgcgca
     9601 ggtaattgcc ggtgcacctt cttcatcata aatatgcaaa taagggctat acggagcgcc
     9661 gcctatagtt ccggtttcgg ttaattcctg tgaggtagta ttcgcatatc caaatccacc
     9721 tgtataacta tcgttaataa tccacttatt agtgcctgtt gctactccat cggggcctgc
     9781 agtgttaagc gtaaaactcc ctgaaggcac attgaagtcc tctgtaaaca gttggaatgt
     9841 ctgagctccg aggctgaagg cagccagaaa acacagtaat gtggtgtaaa atttcatcat
     9901 aggcgtggat aatagctact aaagtaaaga aaaaatgaag aataatgaag gcaaaagcca
     9961 tttctcacca agggagtgct caattttcaa tacaataact caaatagttg ctgtcaacga
    10021 ctttttggtg gggctaacct cattttttat aactttaccc caatgtcact ccgacgtctg
    10081 gcgaatgtta ctgcaattta tgggttgagc acaattttgg tgcgtgtttt aaattattta
    10141 ttagcaccat tacatacacg tgtttttacc gaccagtctg attatggaat tatatcagaa
    10201 atgtatgcat atgttacatt tataaatgta atttgtatgt tcggtttgga gactgccttt
    10261 tttcgttttg cttcaaattc aaccaattcg ctggagaaaa aaactatatt tggcactgct
    10321 caattttcat taattgttag caccatactt atagttggta ctataatttt ttctgcagga
    10381 aatattgccg aattattgga ttacccagga aatgccaatt acatcatatt ttttgcatta
    10441 ataatcggtt ttgatacact tgttaatatt ccatttgcaa aattgcgttt ggaaggaagg
    10501 ccctggaggt atttttctat taaattaata aatattggaa ttaatatttt attgaattta
    10561 tttttccttt ggccggcttt aaaaaataat tacaccttat ttgaaccatt cggatttacg
    10621 taccagcccc aatatggaat tgcctatgta tttattgcca atttaattgc cagcggtgtg
    10681 acttttttac tatttgcgcc aggcatgttt aatattcgtt tcgacttgcc gacatggaaa
    10741 aaattaatgc aatatggtgc tccattaatt attattggtt tggccggaat gatcaacgaa
    10801 accctagaca gagtactgat aaaatactgg ggttccggca ccatggaaga gaacctaaaa
    10861 caggtgggta tttatagcgc ggtttataaa ctctccatct tcatgacact tgcggtacaa
    10921 gggtttagaa tgggtgcaga accctttttt ttcagccagt ccgccgataa aaatgcacct
    10981 tccttatacg ctttaattat gaagtatttt gttgtggttt gttgtgtaat atttttaggc
    11041 gtagggttat tcccggattt atttaaaatc ataattggcg aagagtatca cgaaggttta
    11101 gccatagttc ctattttatt attagcaaat ttatttctcg gtatatatta taatcaaagt
    11161 gtttggtata agctgaccga caaaactgtt tttgccacca taattccatt aacaggtgca
    11221 gcattaactc tattatttaa ttttttgctc attcctcaat taggttatac cggagcagct
    11281 tggacaacat taatatgtta tttcagcatg gtagtaatga gtcatataat tggacaaaaa
    11341 cattatcacg tcccttacaa tttacgcaaa ataatagctt atataataat atctgtagta
    11401 ttatgtttaa ttggcttgtg gttaaataaa atcatatcta atcaacttat ttctattatt
    11461 attcgcgcaa tattcatggg tgtatttttt gtatttgcct ggtggttaga tttacataaa
    11521 ttattgcgta aaacttaatt tatcatcacc tgatatttgc tacaacaaaa cattttttat
    11581 tttatttatt gctccaagta tggaaattaa aatcatcaac aaatcaaata atcccaaccc
    11641 atcatatgcc actgctcaat cggcgggaat ggatttacgt gcaaatcttg agacagaaat
    11701 ggtgctttta cccggacaaa gaatgttgat accaacgggc atttttattg aactcccgat
    11761 tggttatgaa gcccaaattc gtccgcgttc cggtttggca atcaattccg gtataaccat
    11821 gctcaattct ccgggcacaa tagacgccga ttaccggggt gaaataaaag taatcgccat
    11881 taatcacagt gaccagcctt tccacatcaa taatggcgac aaaattgccc aaatggtagt
    11941 cgccaaacat gaatttgtat cctggatcaa cgtcgccaat ttagaaaaaa gcgagcgtgg
    12001 agatgggggg tttggttcga ctgggggttg atgtgctgat gtgattatgt gctgatgtgc
    12061 tgattaatgt accaatgtgt gaatgtaccg atgtaccaat gaaacagccg ttgatgtggt
    12121 aatgtgctga tgtgt
//
LOCUS       DAORKW010000045        11909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_96_length_11909_cov_5.010461, whole genome shotgun sequence.
ACCESSION   DAORKW010000045 DAORKW010000000
VERSION     DAORKW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11909
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96_length_11909_cov_5.010461"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..2063)
                     /gene="acs"
                     /locus_tag="PLB46_03365"
     CDS             complement(147..2063)
                     /gene="acs"
                     /locus_tag="PLB46_03365"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRF75584.1"
                     /translation="MSYPYQIKSFEEYQSAYAKSLAQPTEFWGEIASYFTWRKHWEMT
                     LDWNFVEPHVRWFMGGKLNITENCIDRHLKDRGNQPALIWEPNDPDEAHRVLTYKQLY
                     DKVCQFAHVLRNNGVKKGDRICIYMGMTPELAIAVLACARIGAIHSVIFGGFSAQSIA
                     DRLQDAHAEFIITSDGAYRGGKDIPLKSIIDDALIACPFVKRVIVSTRTRTPISMIKG
                     RDVWWEDEIKKIETLGLHDFEPEIMDSEDPLFILYTSGSTGKPKGVVHACAGYMIYTN
                     YTFINVFQYQPGEIHFCTADIGWITGHSYIVYGPLSAGGTSLMFEGIPTWPDASRFWD
                     IVEKHKVNILYTAPTAIRSLMSFGTAPLEGKNLDSLRVLGTVGEPINEEAWHWYNTNV
                     GKGKCPIVDTWWQTETGGTLISNLAGITPAKPSYATLPMPGVEPVLVDEKGEIITGNN
                     VSGNLCLRSPWPSIIRTTYGDHERCRTNYFATYHNMYFTGDGAYRDENGFYRITGRVD
                     DVLNVSGHRIGTAEVENAINMHAGVIESAVVGFPHDVKGQGIYAFVVYSGDSLADAHL
                     SRQDILQTVTRVIGAIAKPDKIQFVSGLPKTRSGKIMRRILRKIAEGELEQIGDTTTL
                     LDPGVVEEIKDGRV"
     gene            complement(2229..3137)
                     /locus_tag="PLB46_03370"
     CDS             complement(2229..3137)
                     /locus_tag="PLB46_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75585.1"
                     /translation="MNTSPGQHSLTLRSNTLLLLLFLFFLNTIGKAQVPYYEYSKVDT
                     FPQPPLHITLDINTEYAASQFYTLSWSLIPGKNNRILDGTYGEELHCFIYDSKGLVGF
                     YQGDQKPEQFCGFYTTDRVVDVFFRVRPVNEELDDPSYFKELEHFCDYSEFKKIRLIV
                     KHTKSKNVTLYNNNGVVTVRHTNKGAMVPDNMPCYKCCNYDQVLVLSRYYVSTNTGKI
                     LKGSQWLNRKSLYKIVNDLQLVQIDPSFSYFDKKGNQYAGQALIDQHEVPIDSFDMQQ
                     FSEVVDIIPYPQRRLQKIIQSFAKNN"
     gene            complement(3279..5117)
                     /locus_tag="PLB46_03375"
     CDS             complement(3279..5117)
                     /locus_tag="PLB46_03375"
                     /inference="COORDINATES: protein motif:HMM:NF003780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HRF75586.1"
                     /translation="MKILIRIIAVFFVFQNVSIVKGQTATTNSGLLVFRDGVSQPVPE
                     FTDASSWIKEELWVETNFDSDYDNKLDRMHVFVTRPAQTQPGNMQLPIIYSSSPYYGL
                     KLWALLGIGSNKYNWDVKHNFDEEPKSRKHPNLGTRTKRPFMAFYYDNLFVPRGYIMV
                     YSSSPGTGLSDGAPTVGGENESLAPKAVIGWLCGRAKGYTTRTGSETVTAFWSSKKVG
                     MIGTSYDGTLCLAAATTGVEGLEAIIPVAPVSSFYHYYRSNGLVRSPEGFPGEDIDVL
                     YDVITTGDKSKRKNNDRLVRDSILVKNFDRKTGDYNDFWATRDYLNNIDNLHAAVLMA
                     HGFNDWNVMPEQSYRFYKAAKDKGLPVQLYYHQGGHGGDPSITMMNRWFTRYLHGIEN
                     GVENDAPVNIVREFEVEPTAYDSYPDKNAQAVTFFAQSTGNNSGKLILSKGNTVDIDN
                     KISSASLTESENSDHRLLFVTPILKEEIRISGIPTIHLRVAADQPAVNLSVMLVALPW
                     EEGKKVEIYENIITRGWADPQNYQSLTKGEPLQSGKFYDINFDLMPDDQIIPKGQQIG
                     LMIFSSDKEFTIAPKPGAQLMFDVNNISITIPVVGGVEELNAAGIK"
     gene            complement(5402..6079)
                     /locus_tag="PLB46_03380"
     CDS             complement(5402..6079)
                     /locus_tag="PLB46_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75587.1"
                     /translation="MSKFNPFIHDRRSIRLKGYDYSKEGLYFITICCHNRAHLFGKII
                     REEIEPTGSEGIQKMALNEAGQIANDCWLAIPVHFPHAVLHEHVIMPNHVHGIIELIS
                     RPGGAENLPPNNVKDISNDNLFEHDKHLIIHPPKNLELNKSGGANGYSPPQGNFRSPS
                     KTIGSIVRGFKIGVTKWMRQHTKIEEVWQRNYHEHIIRDNRAYENISRYIINNPTKWH
                     IDKFNKK"
     gene            complement(6342..7190)
                     /locus_tag="PLB46_03385"
     CDS             complement(6342..7190)
                     /locus_tag="PLB46_03385"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HRF75588.1"
                     /translation="MRLSPIISTEELLNIYQHPDVLIFDVSTGKNARANYELEHIAGA
                     HFVDLNTQLADIKSDFAGGGRHPLPTVSAFAKTLTDLGISKNKHLIIYDDNNGSNAAA
                     RFWWMLKSAGHEKVQVLNGGINEAKKNNFPMSAETEIIKPANESYVIDQWMLPTIEMQ
                     AVENVSQNPDYLVIDVRSKERYDGKFEPIDLIAGHIPGAINMPFTENLDEQGLFLDPQ
                     VLRNKYEQLFNQIKSENIIVHCGSGVTACHTLLALAYAEMEIPNLYVGSWSEWSRNNK
                     TISTTI"
     gene            complement(7369..8922)
                     /locus_tag="PLB46_03390"
     CDS             complement(7369..8922)
                     /locus_tag="PLB46_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75589.1"
                     /translation="MSRLITMGVLIYILGVQTLSAQYPLDETFGDNGIRFVEWASDMV
                     VLNNGNILLIGSDLDPKKLNVWAFSERGNPLASFGEDGALKLQFSEGCVFRMAIPAEN
                     NEWYMWLTYPVAYYYRDSVIRFNEHGERILDYGINGAAPLPTLNQYIDGNMCILSTGE
                     LVITGELKITDPYVEGRFVYKYTKEGKLDTQFGDEGMVVLNNFTPWVTAPSILIERYD
                     GSLVVGNTIDNGATYFVEIRVIDKWGVPVNSFGQNGVITLKNNASETRLLSYWYDFKS
                     QVLYVHTTGYNDDIRIWQLNWDGELNAFFGTEGYQKSKIMDGSKTMYKVSDGTFYFTG
                     IDFVNNYLQRDIKIWKTNADLDIIPWSEEESAPTLNLQETPDKIQASDEYEIRSVMLH
                     DGSILILTKTLFYGTYAFDYPSAALIKVPPIDQPRTDLCPLLSVPVLYHDQEQSFLQL
                     TLDPLASGDFTFSVTDMMGRTVITHYSLDLAPGISNYTYPIPLELPNGMYLLTIHADD
                     CATGIKFMK"
     gene            complement(9112..9954)
                     /locus_tag="PLB46_03395"
     CDS             complement(9112..9954)
                     /locus_tag="PLB46_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75590.1"
                     /translation="MEKRYITIIIDSITDLNTTNFEIEVSFSVFDDHPDFIELAVESS
                     TQQGISQHDTKLVEMKDDVKSYKGTLKIPIANIVFNIVAHPIIVKPDNERLVKYEDGS
                     DVDNDMKYFPYALVRTTDKNAEFPAPHITEISPFPKTIYGNSGIKITWQTIKKYGEYE
                     LLIRKAGEEAYDTILFKATADNSYKLMSRKLNNVAIDFIMSDLHHLHNFEIQIRGVQP
                     ATVPLETVSSNWSPVVSFHTAIPLNSLSSFLYKKDVITGIRKYFPQNTNVSLRRQMWI
                     DDIY"
     gene            complement(9983..10858)
                     /locus_tag="PLB46_03400"
     CDS             complement(9983..10858)
                     /locus_tag="PLB46_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75591.1"
                     /translation="MPNQDELSARVSFFRREQHPSPLRADFYEGDKFTNGWMPYDAHS
                     AILGVNNVTHSKGVIAGAIDNIPDVGVGGRSDAKFNVGVYGYSENGTGVYCKSVNYEA
                     LHAETQSNETAAIAAYQMNPESNKAAFYAEHANPNKAAAVFKGNVHVHGDILLLNADI
                     AEDFTVSDFETEIGSVMIFGEDGALIPCNRDYDKKVVGVISGAGNYKPGIILDKQKDL
                     NNRLPIALMGKVYCKVDADICPIEIGDLLTTSSTTGSAMKALDPYKSFGTIIGKALMP
                     LNSGKGLIPILVNLA"
     gene            complement(11400..>11909)
                     /locus_tag="PLB46_03405"
     CDS             complement(11400..>11909)
                     /locus_tag="PLB46_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRF75592.1"
                     /translation="KHHDKSTGIWMKIYKKDSGIKTITYAEALDVALCFGWIDGQKQA
                     FDEECWLQKFCPRTTKSIWSKVNIGHVERLIQNGNMRPAGLKVIESAKADGRWEKAYD
                     SPSKMTIPEDFLKALSKNKKAETFFKSLNKTNLFSIGFQLQTAKKQETRDKRMKVIIE
                     KLAKGEKFH"
BASE COUNT         3640 a         2415 c         1930 g         3924 t
ORIGIN      
        1 gaataatgag taatcgcgtt aaatcacaat ttacttgcat ttttggaaca accgaaacca
       61 cagagttacg aaaaaaacag cccaccaaga acccaacagc cgttcaactt caactaaatt
      121 cactaccaaa accacttacc ttgttgttaa accctaccgt ccttaatttc ttccaccact
      181 cccgggtcga gtaacgtagt agtatcacca atttgctcga gttcgccttc tgcaattttt
      241 cgaagaattc ggcgcatgat tttaccactg cgggttttgg gtaaacctga aacaaactga
      301 attttatccg gtttggcaat ggcgccaatt acacgggtta ccgtttgtaa tatgtcttgt
      361 cgcgacaaat gtgcatctgc taacgaatca ccggaataaa ccacaaaagc ataaatgccc
      421 tgccctttaa catcatgcgg gaaaccaact acggcacttt cgataactcc cgcatgcata
      481 ttgatagcat tttctacttc ggcagtgcct attcggtgac cgcttacatt taatacatca
      541 tcaacgcgtc cggtaatgcg ataaaatcca ttttcatcgc gataagcacc atcacctgta
      601 aaatacatat tgtgatatgt agcaaaataa ttggtgcggc aacgttcgtg gtcaccataa
      661 gttgttctga taatagaagg ccaaggtgaa cgcaaacata aattaccact cacattattg
      721 cctgtaataa tttcaccttt ttcatcaact aaaacgggtt caactccggg catcggtaaa
      781 gtggcatacg aaggttttgc cggcgtaatt cctgctaaat tcgaaattaa agtcccgccg
      841 gtttcagtct gccaccaggt gtcgacaatt ggacattttc ctttgcctac gtttgtatta
      901 taccaatgcc atgcttcttc atttattggt tcaccgacag tacctaacac gcgcaaggaa
      961 tctaaatttt ttccttccaa aggtgccgtt ccaaaactca ttaaactgcg aatggctgtt
     1021 ggtgccgtat ataaaatatt tactttatgt ttttcaacaa tatcccaaaa acgggaagca
     1081 tcaggccaag ttggaatacc ttcaaacatc aacgaagttc cacctgcact tagtgggcca
     1141 taaacgatat aactatgtcc cgtaatccaa ccaatatcag ctgtacaaaa atgaatttct
     1201 ccgggttgat attgaaatac attgataaat gtataattgg tataaatcat ataacctgca
     1261 catgcatgca caaccccttt tggtttgccc gttgagccgc tggtgtataa aataaataaa
     1321 ggatcttcgc tatccataat ttcaggttca aaatcatgaa gtcctaatgt ttctattttt
     1381 tttatttcat cttcccacca aacatcgcga cctttaatca tactgattgg tgttcttgtg
     1441 cgggtgctga caattactct ttttacaaac ggacaagcaa ttaatgcatc gtcaataata
     1501 ctttttaatg gaatatcttt tcctccacga taagcaccat cgctggtaat aataaattcg
     1561 gcatgtgcat cttgtaatct gtccgcaata ctttgcgcac taaatccacc aaaaataaca
     1621 gagtgaattg ccccaattct cgcacaagct aaaactgcaa tggcgagttc gggagtcatg
     1681 cccatataaa tacaaatacg atcacctttt tttacaccat tgttgcgtaa aacatgtgcg
     1741 aactggcaaa ccttatcata tagttgtttg taagttagca cacgatgggc ttcatccggg
     1801 tcattcggtt cccaaattaa ggcaggctga ttgccgcgat cttttaagtg tcggtcaata
     1861 caattttcag tgatatttaa tttgccaccc ataaaccaac gcacatgtgg ttctacaaaa
     1921 ttccagtcga gggtcatttc ccaatgtttg cgccaggtaa aataagaagc aatttcaccc
     1981 caaaattcgg ttggctgcgc taaacttttg gcgtatgcgg actgatactc ctcaaaggat
     2041 ttgatttggt atggataact cattttagaa aattgatata acaaagataa tagcgactgc
     2101 agttggcagt tagcacaatt atatcaaatt taggaggatt gtatcaattt gcggatatta
     2161 agcaagtaat tttaaacaac aactgcttta agatgtaaga actaaataat ctgataagca
     2221 caaaatcatc aattgttttt agcaaaagat tggattattt tttgcaatcg tctttgagga
     2281 tagggaataa tatcaacgac ttcagaaaat tgttgcatat caaacgaatc gattggaacc
     2341 tcatgttggt caattaatgc ttgcccagca tattgattac cctttttatc aaaataggaa
     2401 aaggatgggt caatttgaac taactgtaaa tcgttgacaa ttttatacaa acttttacga
     2461 tttaaccact gacttccctt taatattttt ccggtgtttg tactcacata ataacgagac
     2521 aacactaaca cctggtcgta gttacaacat ttgtaacaag gcatattatc cggaaccatg
     2581 gctcctttgt tggtatgcct caccgtaaca acaccgttat tattgtagag cgttacattt
     2641 ttactttttg tatgttttac aataagtctg atttttttga attcgctata atcgcaaaag
     2701 tgctcgagtt ccttaaaata gctaggatca tcaagctcct catttaccgg tcgcactctg
     2761 aaaaatacat ctacaactct gtctgttgtg taaaacccgc aaaattgctc cggcttttga
     2821 tcaccttgat aaaacccaac caaccctttt gaatcataaa taaaacaatg taattcctcc
     2881 ccataagtcc catccaaaat cctgttattt tttccgggta ttaacgacca cgacaaggta
     2941 taaaattgtg aagcagcgta ttctgtatta atatccaacg ttatgtgcaa cggtggctga
     3001 gggaaggtat ctacttttga atattcgtaa tacggaactt gcgctttgcc tattgtgttg
     3061 aggaaaaata aaaataacaa caacaataat gtgttacttc gtaatgtcaa actgtgctgg
     3121 cccggactag tgttcatgaa tttaggaaat aataacttca aaactattaa aaaaaatttg
     3181 gatcagttta aaattatgtc atagtaatat caaaatgagt ttagcaaaac gaaattttta
     3241 atttacgtta aagggttaaa ttaaaaggca gcatcatatt attttattcc cgctgcattt
     3301 aattcctcta ccccacctac aaccggaatc gttatcgata tattgttcac atcaaacatc
     3361 aattgcgctc ccggttttgg tgctatggta aattctttat cactcgaaaa aatcatcaaa
     3421 ccaatttgtt gacctttcgg aattatttgg tcatcaggca tcaggtcgaa gttgatatca
     3481 tagaattttc ctgactgtaa aggttcacct ttggttagtg attgataatt ttgtgggtcg
     3541 gcccagcctc tggtaataat attttcatat atctccacct tttttccttc ttcccatggc
     3601 aatgccacca acattaccga taaatttaca gcaggctgat cagcagcaac gcgcagatga
     3661 attgttggaa ttccggaaat acgaatttcc tctttcaata tcggcgtaac aaacaacaaa
     3721 cgatggtcac tattttcact ttccgttaat gatgcactgc tgattttatt atctatatca
     3781 acagtatttc ctttcgataa aattaattta ccgctattgt tgcccgtcga ttgagcaaaa
     3841 aatgtgacag cctgcgcatt tttatccgga taactatcgt aagcagttgg ttccacttca
     3901 aattcacgga caatattcac aggcgcatca ttttcaacac cattttctat accgtgcaaa
     3961 tatctcgtaa accagcggtt catcattgta atagacgggt cgccaccatg tccaccctga
     4021 tgataatata attgcacagg taatccttta tccttcgctg ctttataaaa acgataactc
     4081 tgttcaggca tcacattcca atcattaaat ccatgtgcca tcaaaacagc ggcatgcaaa
     4141 ttatctatat tatttaaata atcacgtgtt gcccaaaaat cattgtaatc acccgttttt
     4201 ctgtcgaaat tttttactaa aatactatcg cgtactaatc ggtcattatt ttttcttttg
     4261 cttttatctc cagttgtaat tacatcatat aaaacatcaa tatcttctcc cggaaatccc
     4321 tccggcgaac gcaccaatcc attcgaacga taataatgat aaaaagaaga gacaggtgcc
     4381 acaggaataa tcgcttccaa accttccaca cctgtagtag ctgctgctaa acataatgtg
     4441 ccatcgtatg atgtaccaat catacccact tttttcgacg accaaaatgc agttacagtt
     4501 tcagaacctg ttcgtgtggt ataccctttt gctctgccac ataaccaacc aattactgct
     4561 ttaggtgcga gcgattcatt ttctcctcca actgttggtg ctccatccga caaacccgtt
     4621 ccaggcgatg aagaatacac catgatataa cctctgggca caaacaaatt gtcgtaataa
     4681 aatgccataa acggacgttt agtccgcgtg cccaaatttg gatgtttacg cgatttcggc
     4741 tcctcatcaa aattgtgttt tacatcccaa ttatatttat tcgagcctat tcctagtaat
     4801 gcccataatt ttaatccgta atacggactg ctagaataaa taattggtaa ttgcatattt
     4861 cccggttgtg tttgtgcagg tcgcgtaaca aacacatgca tgcggtcaag tttattatca
     4921 taatcagaat caaaatttgt ttccacccaa agctcttcct taatccatga acttgcatca
     4981 gtaaattcag gcacaggttg cgatacccca tctcgaaaaa ccaacaatcc ggaatttgta
     5041 gttgccgttt gcccttttac tattgaaaca ttttgaaaaa caaaaaatac tgctataatc
     5101 ctgattaaaa ttttcatgca ttaaaatttt gtggccaatt tttaaatcga gcagtaaaat
     5161 taaggaatat tcttttttaa aattcagtat ttgggggctt cccggctggc aacatttttc
     5221 ccttaccaat ccaatctcgt gccgggtcgg gcttttcact gcaacaagct gtaacattat
     5281 tattttaatc aaacccggct gttccattag cacataagca cattaccaca ttagcacatt
     5341 agcttgtttc cgttacaatc cctagcctgg ggtgttgtag taaacatgat atttatcggt
     5401 attatttttt attgaattta tcaatatgcc attttgtggg gttgttgatg atatatctgg
     5461 aaatattttc atatgcccta ttatcccgaa tgatatgctc gtgataattt ctttgccaaa
     5521 cttcttcaat ttttgtatgc tgtctcatcc attttgtaac accaattttg aatcctctta
     5581 ctattgaacc tattgttttt gatggtgacc tgaaatttcc ctgtgggggc gaataaccat
     5641 tcgccccacc acttttattc aattccaaat tttttggtgg atgaattatt aaatgtttat
     5701 catgttcaaa caaattatca tttgaaatat cctttacatt attgggcgga agattttccg
     5761 cccctccggg gcgggaaatc aattcaatta tgccatgtac atgatttggc ataataacat
     5821 gttcatgcaa aacggcatgc ggaaaatgga cgggaatggc caaccaacaa tcattggcaa
     5881 tttggccggc ctcatttaat gccatttttt gaatgccctc tgatcctgtc ggttcaattt
     5941 cctccctgat aattttgcca aataaatggg cgcggttatg acaacaaatc gtaataaaat
     6001 acaacccctc tttcgaataa tcatatcctt ttaatcgaat ggatctgcgg tcgtggatga
     6061 acggattaaa tttgctcatt gttttttggg atatcaataa atttttaaat ggtattgaaa
     6121 tatcatttgg attgaaaatg tttattcaat caataaaaac gagaattcga taacattgtg
     6181 aaattatgat aaaccccaaa cggttgttgc aattgttatg tgcattgttt gccgattatt
     6241 cgattacaag cggctttggc tttacagggt tttccattta taatgtggtg tcaatgtggg
     6301 taaattcagg aattcccaaa acttaaaatt ttgtgatgaa ctcaaattgt tgtacttatc
     6361 gttttattat tcctactcca ctcactccac gaaccgacat ataaatttgg tatttccatt
     6421 tcagcataag ccaatgctaa taacgtatga caagccgtaa cgcctgagcc gcagtgcacg
     6481 ataatatttt ccgatttaat ttggttgaat aactgttcat atttatttct taaaacttgt
     6541 gggtcgagaa ataatccttg ctcgtcaagg ttttctgtaa atggcatatt tatagccccc
     6601 ggaatatgtc ccgcaattaa atctataggt tcaaattttc cgtcataacg ttctttggac
     6661 cgtacatcta taaccaaata atcagggttt tgtgaaacat tttctaccgc ttgcatttca
     6721 attgttggaa gcatccattg gtcaattaca tatgattcat ttgccggttt aataatttcc
     6781 gtttcagcac tcatgggaaa attatttttt ttcgcctcat ttattccgcc attcaatacc
     6841 tgcacttttt catgtcctgc tgattttaac atccaccaaa atcttgcagc ggcatttgaa
     6901 ccattattat catcataaat aattagatgt ttgttttttg aaatgcctaa gtcggttaac
     6961 gttttagcaa atgctgaaac agttggcaac ggatgtcgcc cgccaccggc aaagtcgctt
     7021 ttgatatctg ccaattgtgt attaaggtcg acaaaatggg ctccggctat atgttccagt
     7081 tcgtagttgg cacgggcatt tttaccggtg ctgacgtcga aaatcaaaac atcaggatgc
     7141 tgatatatgt ttaataattc ttcagttgaa attattggtg ataggcgcat aggttacttt
     7201 atttttttca atgaaaactg ctgcaaatta actattaatt atatagacac cttttgtgga
     7261 catctaattt ttacgtaaat ctattcgaaa tttttttact ggtgtccaac ttaaaaatga
     7321 agcgaattaa agttactaaa aacaaataag catcaacaat aatatcaatt atttcataaa
     7381 cttaatccct gttgcacaat catcggcatg aatggtgagc aagtacatac catttggtaa
     7441 ctcaagtgga attggatagg tataattcga aatacctggt gcaaggtcta atgaatagtg
     7501 tgtaataacc gtgcgaccca tcatatctgt cacactaaaa gtaaagtcac ccgaagccag
     7561 tggatcaagg gtaagttgta aaaatgattg ttcctggtcg tgatacaaca ccgggacact
     7621 taatagcgga cataaatccg tacgtggttg atctataggt ggcactttaa taagtgcagc
     7681 gctgggatag tcaaacgcat aagttccata aaataatgtt ttggtcagaa ttaaaatact
     7741 gccgtcatgc aacatcaccg agcgaatttc atattcatcc gatgcttgta tcttatcggg
     7801 tgtttcctgc agatttaagg tgggagcact ctcttcctcc gaccatggta tgatatcaag
     7861 atcggcgttt gttttccaaa ttttaatgtc gcgttgcaaa taattattta caaaatcgat
     7921 acctgtaaaa taaaatgttc catcacttac tttatacatg gtttttgacc catccataat
     7981 tttgcttttc tgataccctt ccgtaccaaa aaatgcattt aattcaccat cccaatttaa
     8041 ttgccaaata cgaatatcat cgttatatcc ggtggtatgc acatataaaa cctgtgattt
     8101 gaagtcatac cagtaggaaa gcaatcgggt ttcactggca ttatttttta atgtaatcac
     8161 accgttttgt ccgaaagaat ttaccggcac tccccattta tctattaccc tgatttccac
     8221 aaaatatgtt gctccattat caatggtatt acccaccact aaacttccat catagcgttc
     8281 tattaaaatt gatggtgcag taacccatgg tgtgaagtta tttaatacta ccatgccttc
     8341 atcaccaaat tgggtatcca attttccttc ttttgtgtat ttatatacaa atcttccctc
     8401 aacatatgga tctgttattt ttaattcacc ggtaatcact aattcaccag tagacaaaat
     8461 acacatatta ccatctatat attgattcag cgttggcaat ggtgcagcac cgtttatacc
     8521 ataatccaaa atacgttcac catgttcatt gaaccggatt actgaatcgc ggtaataata
     8581 agcaaccgga taagtcagcc acatatacca ctcattattt tcggcgggaa tagccatcct
     8641 aaatacacat ccttcgctaa actgtaattt caatgcacca tcttcaccaa atgatgccaa
     8701 tggatttccc cgttcactaa aagcccacac attcaatttt ttagggtcca aatcgctgcc
     8761 gattaagagg atgttaccat tattaagcac aaccatatca gaagcccatt caacaaaacg
     8821 aattccattg tccccaaaag tttcatccaa tggatattgt gcactaaggg tttggacacc
     8881 caaaatgtat atcaaaacac ccatggtgat aagccgtgac atacttcaaa tttattaaat
     8941 atgtgggaat ttatatatgc agtaaggaat ttaacattgg ggtgatatac atggccaggt
     9001 tatgttgttt taaaactgta acagggttta gacattattt taacaaaggc ttaggtaaaa
     9061 acaatttgtt ttatgtttgt gtatttaaat ttacataaaa aatatagttt gttaatagat
     9121 atcatcaatc cacatttgcc tgcgcaaact cacatttgtg ttttgaggga aatacttcct
     9181 aataccggta ataacatctt ttttataaag aaaactgctg aggctattta agggtattgc
     9241 tgtatgaaag ctaactacag gtgaccaatt tgaagagacg gtttccaaag gaacagtagc
     9301 cggctgtacg cctctaattt gtatttcaaa attgtgcaaa tgatgtaaat cgctcattat
     9361 aaaatctata gccacattat ttagttttcg gctcattaat ttgtaggaat tgtctgctgt
     9421 agctttaaat aaaatagtat cgtatgcctc ttctcctgcc tttctaatca acaattcata
     9481 ttcaccatat tttttaattg tttgccaagt tatctttatc ccactattac cataaatagt
     9541 ttttgggaaa ggtgaaattt cagttatatg aggtgctgga aattcagcat ttttatcggt
     9601 ggttctaact aaagcataag gaaaatattt catgtcatta tcaacatcag atccatcctc
     9661 atatttaact aatctttcgt tatcaggctt tacaattatc ggatgcgcca caatattgaa
     9721 aacaatgttt gcaataggta tttttaaagt gcctttgtag ctttttacat cgtctttcat
     9781 ttcaaccaat tttgtatcat gttgactaat gccttgttgt gtgctacttt caaccgcaag
     9841 ctcaataaaa tccggatgat catcaaatac tgaaaacgaa acttcaattt caaagtttgt
     9901 tgtatttaaa tcagtaatgc tatcgataat tattgtaata tatcgctttt ccataaaata
     9961 gttttattgt tttataattg gttcatgcca agtttaccaa aataggtatc aaacctttac
    10021 ctgaatttaa aggcattaat gcctttccta ttattgttcc aaaagattta taagggtcaa
    10081 gggctttcat cgcactacct gtggtggaag aagtagtcaa taagtctcca atttcaatag
    10141 gacagatatc ggcatctact ttacaataca cttttcccat caaagcaata ggtaagcgat
    10201 tgtttaaatc tttttgttta tctaaaataa tacctggttt gtagttacct gctccagaaa
    10261 taacaccaac aactttttta tcataatctc tattgcatgg tatcaaagct ccatcttcgc
    10321 caaaaatcat tactgaacct atctcagttt caaaatcaga aaccgtgaaa tcttctgcaa
    10381 tatctgcgtt aagcaaaagt atatcaccat gcacatgaac atttccttta aatactgctg
    10441 cagccttatt cggattggca tgttcagcat aaaatgctgc tttgttactt tcaggattca
    10501 tttgataggc ggcaattgca gcggtttcat tcgattgtgt ttctgcatgt agggcttcat
    10561 aattaacgct tttacaataa acacctgtgc cattttcgct gtaaccataa acgccaacat
    10621 taaattttgc atcacttctg ccacctactc caacatcagg aatattgtca atagcacccg
    10681 ctattactcc tttggaatgt gtaacattat tcactccaag aattgcagaa tgtgcatcat
    10741 atggcatcca tccattagta aatttatcac cttcataaaa gtctgctctc agtggtgatg
    10801 gatgttgttc tcttctaaag aaactaacgc gtgctgataa ttcgtcttga ttaggcataa
    10861 aataattatt ttaaatgtta aaaaaaaggt tttgtaagct tgaaatttcg aagtatcaat
    10921 tttcatgaca caactttatt gaaaagcaac taccttgttt tgttaatcca aaaaaatcct
    10981 tgaggataag tgtagcaact cgattcatcg tgcaatttat accctaattt tcaatataaa
    11041 gaaattttaa tgcaaattca cggtataccg ttaatcctca aaatggagta tgcgctgggc
    11101 aaggtcatag caaattcacc aagcccaagt tgtatacaaa cgaattataa aacattacaa
    11161 tgaaaattat agcaaattgt gtgatacaca tcaattgcat ttccatattc aactttcggt
    11221 aactatctta ctatatccag ataaattaca attagagaat aatttgaata ggccgtaatt
    11281 acaagtttga ggaaagaatt aatctttagc aatacataac cttaggaagc ccattatttt
    11341 tgttatgcaa ggagggtttc aaccagttta acaaacacaa acctacatct ttcaatattt
    11401 caatgaaact tctccccctt tgctaacttt tcaataataa ctttcattct tttatcgcgt
    11461 gtctcttgtt ttttggctgt ttgcaattga aatccaattg agaataggtt cgttttattg
    11521 agacttttaa aaaacgtttc tgcctttttg tttttactga gtgcttttaa aaaatcttcc
    11581 ggtatggtca ttttacttgg ggaatcgtac gccttttccc atctgccatc tgctttggca
    11641 ctttcgataa cttttaatcc ggccggtcgc atgttgccat tttggattaa tctttccacg
    11701 tgaccaatat tcactttaga ccaaatactt tttgttgttc tcggacaaaa tttttgtagc
    11761 caacactcct cgtcaaatgc ctgtttttga ccatcaatcc aaccaaaaca taatgcaaca
    11821 tccaatgctt ctgcataagt tatcgttttg atgccggaat cttttttata tattttcatc
    11881 caaattccgg ttgatttgtc atggtgttt
//
LOCUS       DAORKW010000046        11854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_98_length_11854_cov_4.442326, whole genome shotgun sequence.
ACCESSION   DAORKW010000046 DAORKW010000000
VERSION     DAORKW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11854
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98_length_11854_cov_4.442326"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..4591)
                     /locus_tag="PLB46_03410"
     CDS             complement(269..4591)
                     /locus_tag="PLB46_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75593.1"
                     /translation="MKLKKLLPFLLIIFISNQIWADTEPDNNTAATADAMTLDVGNTG
                     TINITGDVIDYYAFSTATDGKITLSLTSDVTYLTIRLYDSDGATQLGTESNYSTVALS
                     VDGLAAGNYYAAVVAYAGYSGNYTITSSLTIAEYANDTEVNDTYLQAIDIAENGNATG
                     HIGFRNNGGAYDMNDWYKVVTTQDGNITATFANNTGDYNNLYIYDNDGTTLLGSTADY
                     GTDTLTVTGKAAGTYYIRLNYYSTNYFNGYTITNTVTPTIFANDAEPNDTYASATVTI
                     PENGSVNGHIGHRYNGGSYDTDDWYILNMSQDGQLQLNLTQGAGMYHGIYLYDNDGTT
                     LLASNFEYTAVSTVRYNLSAGTYYAKVDNYSTSYYSGYTLQFVATPTAFASDVEPNEL
                     YTDATVSIPEGGTVNGHIGFYRNGGTYDTDDWYKLTTTEDGIITLTINQGTGMYHGIY
                     LYDNNGTTLLGSEFNYTTATFSVNNLAAGDYYAKVDNYSTSYYSSYTLSFNLTPPVYA
                     NDPEPNGSLAEAAPMLTNSTVEGHIGYRSNGTIYDQNDYYIINISEPGDLTLTIEKDP
                     AQYNTIYILNSAGTTLESQSNYGTFSISELNLAAGTYYARVNYYSTLHFGPYRLTNNY
                     CPDQITIVAEGETTFCDGESIILSTPDHHLSYLWSDGSVTETNTVTLSGDNYLTIDNG
                     AGCVRTSNTITTESTPLPVAIIEADGATEFCEGGDVTLSVPIVADSYLWSNGATTPTI
                     TVSATGDYSVELTKNSCSAISDPIHITVNANPVAAITPDGATTFCEDGSVTLTANDAE
                     SYEWSNGATTNSIIADASGDFYVTITDANGCSNTSSTISVTENANPIAGISADGPTTF
                     CSGGSVNLIATGGVTYLWNTGATTSSINVTTSGVYSATVYSAEGCSNNSSTIEVIVEA
                     CGSVSIAAEGPVEFCEGGAVILTSTEADGNIWNTGETTQSIVVTESGDYYCTNGVNTS
                     NTISVTVNANPIATISADGATEFCEGGSVNLTASAANLYDWNTGETTASILVTTNGDY
                     AVTVTDANGCSATSATTTVTVNSNPTATISADGATTFCEGGDVNLMASEATSYLWSNG
                     STDGTINVSASGDYSVMVTDANGCSATSDNITVTENANPTPVITADGPVVFCGTDVTL
                     NTDGVYDTYLWSNGETTSSITTAISGAYSVTVTSGLCEGVSNTIDVTADAAPSVSITS
                     EGTLICYEASTMLTATTDAANIQWQRNGIDIPGANDATYYASLNGKYRAVVTTGECEA
                     TSLDIKLKYAERLEITPSGTVGLCGGDVIMSVPAVMGATYQWYQNNVALGGATSNSYT
                     TSIKGKFYCLATKEGCSSASKLLVVTDGCREGELTQAEMMVMPNPASDNFEMVFTALQ
                     AGTVTIQITTITGSIIVSENVEVIEGEQTRNYTTENMPSGMYLVNMQSANGAVITQKI
                     IIEK"
     gene            complement(4696..5334)
                     /locus_tag="PLB46_03415"
     CDS             complement(4696..5334)
                     /locus_tag="PLB46_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF75594.1"
                     /translation="MPIRVAIFDDNATRRDGVKLLLDTSEGFLCAGAFENFNNGVADI
                     LSCNPDVIIMDIDMPGINGIEAVRMIKTNYPQLPVMMQTVFDHDDKVFDSILAGASGY
                     LLKKAAPLKLLEAIREIHEGGAPMTPEIAIKVLHFFRNKEQQKVTNDMLLTEKEKEVL
                     SDLVAGLSYKMIASKQAISYHTVNFHIRNIYKKMHVNSVSEAVAKALKEKMI"
     gene            complement(5393..8461)
                     /locus_tag="PLB46_03420"
     CDS             complement(5393..8461)
                     /locus_tag="PLB46_03420"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF75595.1"
                     /translation="MAYKPLSAQQFPDFHFENISTADGLSDNTITCLFQDNKGFLWIG
                     TGNGLNRYDGNIVTTYFYEEGNVNSMGGNFISGIIQDDAGVFWIGTRDGGITRLDLSA
                     PLTQQFKRFSNIPGDSTTMFSNRITTLAELNADYIVFSAEGISTGFINRKTFEITYHE
                     NKNKSVALVDTKITKPKPDGNSWMQLIKRDGNTVYTSGLIGGTVYAFDRNNPVVPKNS
                     TNGTASSIQNFDVDGDTVWVGAWVHGLFIQENPLGKPIDKPLALKKVVPIDAEVLSVL
                     SWDKNFVLAGSKGNGLYLVNKKTFEYKVFQHDRADAFSLAGNKINCLLRDKNGILWVG
                     TSAGLSKYNTIQWQLNATLIKDDFSKEITHFSIYEFNDHSFGLNTGMGLFRYYPESGT
                     FTQHHFMLNGAEINPTAIVAITDNKHYLTTETNTFYIDLKTLHIEEISPKTLCNPALD
                     TCYRTQDVLFGNYQIYDVLFDTIDNHILHLFTTIGSGVSIYDATDDTYYNLFQYTNKP
                     NSISNNFVRNIYRDSKGNIWVSTSEGLNKWNKSFPIKNDFTIYKHSISDSNSISHNNI
                     SAVWEAPDGILWIATSNGLNAFDGKKFTRYYNTVNNQQQMFGLYPDNKGNLWLPIKGG
                     FLVFNLQDRNFRFVPLIYSGWSLRSPARLLQSKSGTWMYGAGNYLISFNPDQYIFETK
                     FPELYITDMLVSDKLIDKSNDYAELVFKHDENFITVNFSSLQLSQPKTVKYRYQLAGL
                     TDNWTDLGKNNVIRFTSLPPGKYTLYIQVTNPQGYWSNSSNMLTFEILKPYWQTWWFY
                     LLCVLSIFGITFLIIRYREQQLKNVLTMRNKIANDLHDDVGSALSTINLYSEVAKNKS
                     GANKDLEPILDKITGISTEMQENMTHIVWSLQPRNDNFDQMLLRIKSYALENLSVKNI
                     EIHFDIDEKLSGIKIAANKRKELFLIYKEVLNNIQKYANATQVHIQFKRLGGDLRMEI
                     RDNGVGFDVNALNDGNGMFTMRERAAALNGNFEMFSQLGKGTTVVLSFKW"
     gene            8793..9899
                     /locus_tag="PLB46_03425"
     CDS             8793..9899
                     /locus_tag="PLB46_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75596.1"
                     /translation="MKKIDLGIAQNKYTQIAQRIEYIAHLSMAKMLAHADNPSTYPMT
                     SDQNSIEHILFTHYKKLPKSAQNKFRAGIENLIKMPSTQAQRKAKYGDLFEVDFKKNE
                     AVATQIQKMPFPEHLKFTPAENDVVAKKILNKRVINKKTFSIPEPVVAAPRIIRLSVE
                     SIHCVQTVELNKDEILLNGLFTDALGNTTVIDIIDIGKMKNGDTIPLGEKGQLAEVNL
                     NNAGFFPQTLLGTFALIDKDIFADQEKIVDAFKTCAAMAITLTSIGLVSMGIAVGMAL
                     TGGALAIAAGLMTGALVAMIGSLVVLVVGGGITLFLQGESSELINDAFVFDLNPVTLQ
                     PGEFVVRTLNIDIARSIGKKKGNYTMNVKWERIS"
     gene            complement(9994..10647)
                     /locus_tag="PLB46_03430"
     CDS             complement(9994..10647)
                     /locus_tag="PLB46_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF75597.1"
                     /translation="MKIIITGATGMVGEGVLLVCLQHPDVSQVLSVSRKPQAHKHAKL
                     KELIVRDFSELEKYKTELQSYDGCLFCAGVSSIGETEATFTNKTFDFVVPFGKLLAEI
                     NPQLTFIYVSGSGTDSSEKGKVMWARVKGRTENTLSKLPFKGVYHFRPGLMKPVAGQK
                     NVKPIFRVVTALYPVVKVLAPKWTCTLDQVGTAMLHCVEKGYTKSILEVEDIKKIGG"
     gene            complement(10905..11249)
                     /locus_tag="PLB46_03435"
     CDS             complement(10905..11249)
                     /locus_tag="PLB46_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HRF75598.1"
                     /translation="MENKQEMVQMKTLTASLNSFYLKGYTENYKVDNLGLKALSTEKI
                     YQPDDVKVLDFHRFEGPTDPADESILYAIETEDGCKGTLVDAYGPYADSKITSFMQRV
                     EEISKNTTEKGS"
     gene            complement(11252..11428)
                     /locus_tag="PLB46_03440"
     CDS             complement(11252..11428)
                     /locus_tag="PLB46_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HRF75599.1"
                     /translation="MVKKSKKSPGKMEKVMHEFKEGTLKSGKDGKGGKVTNRKQAIAI
                     GLSEERGAKSKKKK"
     gene            complement(11606..>11854)
                     /locus_tag="PLB46_03445"
     CDS             complement(11606..>11854)
                     /locus_tag="PLB46_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75600.1"
                     /translation="LYNYGATGLQTFLESNFHKATGLHQIIPSGDDFIFYTTALSFKE
                     GGVVRSGFEIINNDVIFSISISPHFENFKVTCGKIKLQ"
BASE COUNT         3727 a         2343 c         1941 g         3843 t
ORIGIN      
        1 tattattaca cccgatggca gagttcgtgt caaagctttc cgtctctcag aatacgatgc
       61 catcattgct aaaaatgatg taaaaacagg tgtgggtatt tattatacca aagatttcaa
      121 taaaataaaa gagttgttgc cttggaataa ataatatggt aacaattaaa agctagagaa
      181 agagtttgtt ttaatccgga ttatacaatt tcacatcata aaaaaagccc ccacctttca
      241 gcaggggcta tggacttgtt ttaaaaaact atttttctat aataattttt tgtgtaatta
      301 ctgcaccatt ggcagactgc atgtttacta aatacatccc tgaaggcata ttttcagtag
      361 tataattgcg tgtttgttca ccttcaatta cttcaacatt ttcgctaacg atgatacttc
      421 cggtaatagt agttatttga attgttacag tacccgcttg caaagcagtg aataccattt
      481 cgaaattatc agaagcaggg tttggcataa ccatcatttc cgcttgagtt aattcgcctt
      541 cgcgacaacc atctgtaacc acgagtaatt ttgatgcact ggaacaacct tctttagttg
      601 ccaggcaata aaatttacct ttgatagatg ttgtataact attagaagta gcgccaccaa
      661 gagcaacatt attttgatac cattgataag tagcacccat aactgcaggt acactcatta
      721 ttacatctcc accacataaa ccaactgttc ctgaaggtgt aatttccaaa cgttcggcgt
      781 atttcaattt aatatctaaa cttgtagcct cacattcgcc tgtagtaacc actgcacgat
      841 attttccatt taaggaagca taataagtgg catcattcgc accaggaata tcaatgccat
      901 ttctttgcca ttgaatatta gcagcgtcgg ttgttgctgt taacatagta ctagcttcat
      961 aacaaattaa tgtgccttca gaagttatag aaacagaagg tgctgcatca gcagttacat
     1021 caatagtatt tgaaacacct tcgcataatc cggaagtgac agtaactgag taggcgccag
     1081 aaattgcagt agtaatagat gaagttgttt caccattact ccataaataa gtatcataaa
     1141 caccatcagt atttaacgtc acatcagttc cacaaaatac aacaggacca tctgccgtaa
     1201 ttacaggtgt cggatttgca ttttcagtaa ctgtaatatt atctgaagtt gcagaacatc
     1261 cgttagcatc ggtaaccata actgaataat caccggatgc acttacattt attgttccat
     1321 ctgttgagcc attgctccat aaataagatg ttgcttcaga tgccattaaa ttaacatctc
     1381 caccttcaca aaaagtagta gcgccatcag cagaaattgt tgctgtaggg tttgaattaa
     1441 cagttacagt tgtggttgca gaagttgctg aacaaccatt agcatctgtt accgttactg
     1501 cataatcacc atttgtagtt accaaaatag atgcagtggt ttcgccggta ttccaatcat
     1561 ataaatttgc cgcacttgct gttaaattta cacttccacc ttcacaaaat tcagttgccc
     1621 catcagcaga aatagttgct attggatttg cattaacggt tacagaaatg gtgtttgaag
     1681 tatttactcc attggtgcaa taataatcac cactttcagt taccacaata ctttgtgttg
     1741 tttctccggt gttccagata ttaccatcag cttctgttga agttaagata acagcaccac
     1801 cttcgcaaaa ttcaacaggc ccttctgctg caattgaaac agaaccgcaa gcctcaacaa
     1861 ttacttcaat tgtgctacta ttattactac atccttcggc actatacact gttgcagaat
     1921 aaactcccga agtagttaca tttattgatg atgttgttgc accggtattc cataaataag
     1981 taacaccgcc tgttgcgatt aaattaacag aacctccact acaaaacgtt gtcgggccat
     2041 cagcactaat tcctgcaatt ggatttgcat tttcggttac acttattgtt gacgatgtat
     2101 ttgaacaacc atttgcatca gtaatggtta cataaaaatc accactcgca tctgcaataa
     2161 ttgaatttgt tgtagcacca ttactccatt cgtaggattc ggcatcattt gctgtaagtg
     2221 tcacacttcc atcttcacaa aaagttgtcg caccatcagg tgtaattgcc gcaacaggat
     2281 ttgcatttac agtgatgtgt atcggatcag agatagcaga gcaactgttt tttgttaatt
     2341 caacactata atcacctgtt gcacttacgg tgatagttgg tgttgttgcg ccattgctcc
     2401 ataaataact atctgcaaca attggtacac ttaaagtaac atctccccct tcacaaaatt
     2461 cagttgcacc atcagcttct attattgcaa ccggtaacgg tgtagattct gtagtaattg
     2521 tatttgaagt gcgcacacat ccagcaccat tatctatagt taagtaatta tctcctgata
     2581 aagttactgt attcgtttct gtaacggaac catcactcca caaataactt aaatgatgat
     2641 caggtgtact taaaattatg gattcaccat cacaaaaagt agtttcacct tcagcaacta
     2701 tagtaatttg atcagggcaa tagttgtttg ttaaacggta tggtccaaag tgtaatgtgc
     2761 tgtaataatt tacacgagca taataagtgc cggcagctaa atttaattct gaaattgaaa
     2821 aagttccata atttgattgc gattctaaag tagttccagc actattcaaa atatagatgg
     2881 tattatattg cgccggatcc ttttcgatag ttaacgttaa atcacccggt tcacttatat
     2941 taataatata ataatcgttc tggtcgtaaa tagtgccatt acttctataa ccaatgtggc
     3001 cttcaactgt agaattggta agcattggtg ctgcttccgc caacgaacca tttggttccg
     3061 ggtcattagc ataaacaggt ggagttaaat taaacgataa agtgtaagat gaataataag
     3121 atgtactata gttatcaacc tttgcataat aatcgccggc agcaagattg ttcacagaaa
     3181 aagtggcagt agtataatta aattcactac ctaataaagt tgtaccatta ttgtcataca
     3241 aataaatacc atgatacatt cctgtcccct gatttattgt taaggtaata attccatcct
     3301 cagttgtggt tagtttatac cagtcatcag tgtcataagt accaccgttt ctataaaatc
     3361 cgatatgtcc gtttactgtt ccaccttcag gaatactgac cgttgcatcg gtatataatt
     3421 cattaggttc aacgtcagat gcaaaagccg ttggtgttgc cacaaattgt aaggtataac
     3481 ctgaataata agatgtagaa taattgtcaa ctttagcata ataagttcct gcagataaat
     3541 tatatcgaac tgtgcttaca gcagtatatt caaaattaga tgcaagcaaa gttgtgccat
     3601 cattatcata taaataaata ccgtgataca tgccggcacc ttgtgtcaag ttaagttgta
     3661 actgaccatc ctgactcata tttaaaatat accagtcgtc agtatcataa gagcctccat
     3721 tataacgatg accaatatgt ccgtttacac tgccgttttc aggaattgta actgtagcag
     3781 atgcataagt atcattcggt tctgcatcat tagcgaaaat tgttggtgta acggtattcg
     3841 ttattgtata accattaaaa taattagtag aataataatt taaacgaatg taatatgttc
     3901 cggctgcttt tccggtaact gttaatgtgt cggttccata atctgctgtt gaacctaaca
     3961 atgttgttcc atcattatca taaatgtaaa ggttattgta atctccggta ttatttgcaa
     4021 aagttgcagt aatatttcca tcctgtgttg taacaacttt ataccagtcg ttcatgtcat
     4081 aggcaccgcc attatttcga aaaccgatat gtcctgttgc attaccgttt tcggctatat
     4141 caatggcttg caaataagta tcattcactt ctgtatcatt ggcatattcg gcaatggtta
     4201 aactggaagt aatggtgtaa tttcctgaat aacctgcata tgcaacaact gctgcataat
     4261 aattacctgc tgctaaacca tcaactgaaa gtgcaacagt cgaataattt gattcagtcc
     4321 ctaattgcgt agctccgtcg ctatcataca accgaatggt aagataggtt acatcgctag
     4381 taagcgataa agttatttta ccatctgtgg cagttgaaaa cgcatagtaa tctatgacat
     4441 ctcctgttat gttgatagtg cctgtgttgc caacatcaag tgtcatggca tctgcagtag
     4501 ctgcagtgtt gttgtccggc tctgtgtcgg cccaaatttg attgctgata aaaataatca
     4561 gcaagaatgg gagtagtttt ttgagtttca taaaatagtt tattggtttg ctcacaagta
     4621 tgagaaattt gagaaactca aaattaagtg caaagccctg cctgtaaaac agcgaatagt
     4681 agtgggaaaa tcagctcaaa tcattttttc ttttaacgct tttgcaacag cttcacttac
     4741 actgttaaca tgcatttttt tgtagatatt ccgaatatga aaattaacag tatgatagct
     4801 aatggcttgt tttgaagcaa tcattttata acttaatcct gcaacaagat cagataatac
     4861 ttctttttct ttttcagtaa gcagcatatc attcgtcact ttttgctgtt ctttatttct
     4921 gaaaaaatgt aacactttaa tggcaatttc gggtgtcatt ggcgcacccc cttcatgaat
     4981 ttcacgaatc gcttctaaca gttttaacgg cgctgctttt ttgagcaaat accccgaagc
     5041 acctgctaaa atactatcaa aaaccttgtc atcgtggtca aaaaccgttt gcatcattac
     5101 cggtaattgc gggtaattcg ttttaatcat ccttactgcc tctataccat ttataccggg
     5161 catgtcaata tccatgataa tcacatcagg attacaggaa agtatatcag caacaccatt
     5221 attaaaattt tcaaatgccc ccgcacataa aaagccttca gaagtatcca ataataattt
     5281 aaccccatct cttctggtgg cattatcatc aaatatggct acacgaatcg gcataaaatc
     5341 atatcaaaat tatctaataa tgttgattac ctacctacta atattagtag gtttaccatt
     5401 taaacgaaag cacaacagtg gtgcctttac cgagctgtga aaacatttca aaattgccat
     5461 ttaatgccgc agccctttct cgcatagtaa acatgccgtt tccatcattc aacgcattta
     5521 catcaaaacc aacaccatta tcccgaattt ccatgcgcaa atcgccaccc agtcgtttaa
     5581 attgtatatg cacttgtgtt gcgtttgcat atttctgaat gttatttaat acttccttgt
     5641 aaatcaaaaa taactctttg cgtttattag cagcaatttt aattccactt aatttttcat
     5701 ctatatcaaa gtgaatttca atatttttca ccgataaatt ttccagcgca tatgatttta
     5761 tacgtaacaa catctgatca aagttgtcgt ttctcggctg caaactccaa acaatatgtg
     5821 tcatgttttc ctgcatttcg gtgctgatgc cggtaatttt atctagaatc ggttccaggt
     5881 ctttattcgc tcctgattta tttttggcta cttcactgta taaattaatt gtacttagag
     5941 cgctgcccac atcatcatgc aaatcgttcg caattttatt acgcatagta agcacatttt
     6001 ttaattgctg ctcgcgataa cgaataatta aaaatgtaat tccaaaaatg cttaacacac
     6061 acagcagata aaaccaccat gtttgccaat agggtttcaa aatttcaaaa gtcagcatat
     6121 tactgctgtt gctccagtag ccttgcggat tggtaacctg tatatacaag gtatatttcc
     6181 ccggaggtaa actggtaaac cgtatcacat tattttttcc caaatctgtc cagttatcag
     6241 ttaatcctgc tagttggtag cgatatttta ctgttttagg ttgtgacagt tgtaaactgg
     6301 aaaaattaac agtaatgaag ttttcatcat gtttaaacac cagttcggcg taatcattac
     6361 ttttatcgat taacttatca gaaaccaaca tatcggttat gtataattcc ggaaatttgg
     6421 tttcaaaaat atattggtct gggttaaagg aaattaaata attgcctgcg ccgtacatcc
     6481 atgtgcccga tttgctttgt aataacctcg ccggtgaacg caacgaccaa cctgaataaa
     6541 taagtggcac aaagcggaaa ttcctatcct gtaaattaaa cactaaaaag ccccctttta
     6601 ttggcaacca aaggtttccc ttattgtccg gatacaatcc aaacatttgc tgctggttat
     6661 ttaccgtatt gtaataccgc gtaaattttt tcccgtcaaa tgcatttaat ccatttgagg
     6721 tcgcaatcca taatattccg tccggagcct cccatactgc ggaaatatta ttatgagaaa
     6781 tggaattaga atcagatata ctgtgtttat aaatggtgaa atcattttta atgggaaacg
     6841 atttattcca cttatttaat ccctcagatg ttgacaccca aatgttacct ttagaatcgc
     6901 gataaatatt tcggacaaag ttattggata tactattcgg tttgttggta tattggaaca
     6961 ggttataata agtatcatct gtggcatcat atatacttac gccagaacca atagtagtga
     7021 acaggtgcaa aatgtggtta tcaatagtgt caaacaatac atcataaatt tggtaattcc
     7081 caaacaaaac atcctgtgtc ctatagcatg tatctagcgc aggattacaa agtgttttag
     7141 gagatatctc ctctatatgc aaggttttta aatcgatata aaaggtattt gtttcagttg
     7201 ttaaataatg tttattatct gtaatcgcaa caatcgcagt tgggtttatt tcagcaccgt
     7261 taagcataaa atggtgttgt gtgaatgttc cactttcagg ataatatcta aacaaaccca
     7321 ttcctgtatt taacccaaat gaatggtcat taaattcata tatcgaaaaa tgggtaattt
     7381 ctttagaaaa atcatcttta attaaagtcg cattcaattg ccattgaata gtattatatt
     7441 tacttaatcc tgcagaagtc cccacccaca aaataccatt tttgtctctt aataaacaat
     7501 taattttatt tcccgccaaa ctaaaagcat cagctctatc gtgctgaaat actttatatt
     7561 caaacgtttt tttatttaca agatataatc cattaccttt tgaaccggca agcacaaaat
     7621 ttttatccca cgacaatacc gataaaactt cagcgtcaat aggcactact tttttaaggg
     7681 caagcggttt gtcaattggt tttcccaacg gattttcctg aataaataaa ccatggaccc
     7741 atgcgcctac ccaaactgta tcgccatcaa catcaaaatt ctgaatactg gaagctgtcc
     7801 catttgtact gtttttcgga acaactggat tatttctatc aaatgcataa acagtaccac
     7861 caatcagccc tgatgtataa accgtattac catcacgttt tattagttgc atccaactgt
     7921 ttccatccgg tttaggtttt gtaattttag tatctactaa tgcaactgat ttatttttat
     7981 tttcatggta ggtaatttca aatgtttttc tgtttataaa accggtggaa ataccttctg
     8041 cactaaatac aatataatct gcatttaatt ctgctaaagt ggtaatacgg tttgaaaaca
     8101 tagtagtgga atcacctgga atattgctga accgcttaaa ttgctgcgtt aaaggtgcac
     8161 tcaaatccag tcgcgtaatg ccaccatcgc gtgtgccaat ccaaaacaca cctgcatcat
     8221 cctgaataat acctgaaata aaattgcctc ccatggaatt gacatttccc tcctcataaa
     8281 aataggtggt tacaatatta ccgtcatatc ggtttaatcc atttcctgtg cctatccata
     8341 aaaagccttt attatcctga aataagcaag taatggtatt atcgctcaag ccatcagcag
     8401 tgctaatatt ttcgaagtgg aaatctggaa attgttgggc actcagcggt ttataagcca
     8461 tcaagcaaac aatcaatatc aacccatatt tgaaacccaa ttttagcatg gtaagctaag
     8521 atacgaaagt gaatatattt ccttgcattt gatataacat acctatgtaa agtatcaaaa
     8581 gttaaaaata tcaattatta aaaatgtaac aattatgcac ccatattacg acgtaaatca
     8641 aataaacaaa ataggatttt ttaatcagga aattgaacaa aagttgcgtt acagcctcat
     8701 ctaccaccca tcacatcttg atgtgatatt tcaattttcc taggtatttt gcaagtgctc
     8761 atactacatt tggactaaca aaatgtaagc gtatgaaaaa aatagacctg ggtattgctc
     8821 aaaacaaata cacccaaata gctcaacgca ttgaatatat tgcccattta tcaatggcaa
     8881 aaatgcttgc tcatgccgat aatccttcaa cctatccaat gaccagcgac caaaattcga
     8941 tagagcatat tttatttacg cattataaaa aattacccaa atcagcacaa aataagttta
     9001 gagccggtat cgaaaacctg attaaaatgc cttccactca agctcagcgc aaagctaaat
     9061 atggagacct gttcgaggtg gacttcaaaa aaaatgaggc agttgccacg caaatccaaa
     9121 aaatgccctt cccggaacat ttgaaattta ctccggcaga aaatgatgta gttgcgaaaa
     9181 aaattttaaa taaacgggta ataaataaaa aaacgttttc catccctgag cctgttgttg
     9241 ctgctcctcg catcattcga ttatctgtgg aaagtataca ttgtgtgcag acagttgaat
     9301 taaataaaga tgaaattttg ctcaacggat tatttacaga tgcgcttggc aatactacag
     9361 taattgatat tatagatatc ggcaaaatga aaaatggcga caccattcca ttaggcgaaa
     9421 aagggcaatt agctgaggta aatttaaata atgcgggatt tttcccacaa acattattag
     9481 gtacatttgc cctaatcgat aaagatattt ttgctgatca ggaaaaaatt gtagatgcct
     9541 ttaaaacctg tgcagctatg gctatcacat taacaagtat tggtttagta tctatgggta
     9601 ttgcagttgg catggcttta accggtggtg cattggcaat agctgccggt ttaatgaccg
     9661 gggcgttggt ggctatgatt ggttcgttgg tcgtgctggt ggtaggtgga ggtataaccc
     9721 tatttttaca aggcgaatct tccgaattaa ttaatgatgc atttgtgttc gatttaaatc
     9781 cggtaaccct gcaacccgga gagtttgtag tgcgaacttt aaacatcgat attgctcgtt
     9841 ctatcggaaa gaaaaaaggg aattatacca tgaatgtaaa atgggaacgt atttcttaat
     9901 atggtttagg gaaataattt ttctatttaa acctattatt aaggtcaata gctatttatg
     9961 ctaattggta tgttgtagcc tgaaatacaa atttcaacca cctatttttt taatatcctc
    10021 aacctccaat atgcttttcg tataaccctt ttccacacag tgtaacatag ccgttccaac
    10081 ttgatccagc gtgcaggtcc attttggtgc aagcacttta accacagggt ataaggcggt
    10141 aacaacgcgg aatatcggtt ttacattttt ttgcccggca actggtttca tcaagcccgg
    10201 tctgaaatga tatacccctt taaaaggtaa tttgcttaat gtattttctg ttctgccttt
    10261 aaccctagcc cacatcactt ttcccttttc actgctgtcg gttccgctgc cggaaacata
    10321 tatgaatgtg agttgtggat ttatttctgc taataatttt ccaaaaggca ccacaaaatc
    10381 aaatgtctta ttggtaaacg ttgcttccgt ttcaccaatc gaactcacgc ctgcacaaaa
    10441 taaacaacca tcgtacgact gcaattcagt tttgtatttt tccaattctg aaaaatcgcg
    10501 gacaatcaat tcttttaatt tggcatgttt atgcgcttgc ggttttctgc tcacagacaa
    10561 aacttgtgaa acatcaggat gttgcaaaca aaccagcaac acaccttcac caaccatacc
    10621 cgtagcaccg gtaatgataa ttttcataca ttattttgcc taaaagtagg caagatttac
    10681 caaatactat tcaaacaaat tttaaataaa acaaattgga gtagcaacat cgcgtttcga
    10741 tagtcaatat cttgcaggtg cttcatttta agaccttctc gcctgcactg cattctgggt
    10801 cctgcgggct tttttcccga ttactatcgg gccgcctcgg gctagctatt catttatcat
    10861 aaacaaaaca tataagtgaa tttttcgctt atttttaaag tagtttatga ccctttttct
    10921 gttgtgtttt ttgaaatttc ttcaacacgt tgcataaaag aagtaatttt cgaatctgca
    10981 taaggtccat aggcatccac cagggttcct ttgcatccat cttctgtttc aatggcatat
    11041 aatatagact catctgccgg gtctgtagga ccctcaaagc ggtgaaaatc caaaaccttt
    11101 acatcatccg gctgataaat tttttctgta gaaagtgcct ttaatcccaa attatccacc
    11161 ttatagtttt ccgtataacc tttcaaataa aaactgttta acgacgccgt taaggttttc
    11221 atctgaacca tttcttgctt attttccata cttatttctt tttcttcgat tttgcaccac
    11281 gttcctcact tagtccaatg gcaatagcct gcttacgatt ggtaaccttt ccacctttac
    11341 catcttttcc cgatttcaat gtaccttcct taaattcatg catcaccttt tccattttgc
    11401 caggcgattt ctttgatttt tttaccataa ttgcatgatt ttaaacttgg ttgaaaatga
    11461 ttacaaatag catttactat agaacaaatc taatctttaa aacacggaca cttttacaca
    11521 attaaattta taaattgtat aattcattca aaccggacaa tgtttatcga atagaattgc
    11581 ggaaatgtta tccgagaagt taaaattatt gtaattttat ttttccgcaa gtcaccttga
    11641 aattttcaaa atggggcgat atactaattg aaaaaataac atcattattg attatttcaa
    11701 atcccgaccg aactacacca ccttctttaa atgaaagcgc agtcgtataa aaaataaaat
    11761 catctccaga tggtataatt tggtgcaaac cagtagcttt atgaaaattt gattctaaaa
    11821 atgtctgtaa tccggtagca ccataattgt aaag
//
LOCUS       DAORKW010000047        11714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_103_length_11714_cov_6.206621, whole genome shotgun sequence.
ACCESSION   DAORKW010000047 DAORKW010000000
VERSION     DAORKW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11714
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103_length_11714_cov_6.206621"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLB46_03450"
     CDS             complement(<1..255)
                     /locus_tag="PLB46_03450"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75601.1"
                     /translation="MKLQSLFLLFIFFNLSCSAQSDNSVASTGYGVMFYNLENLYDTI
                     NDPAKDDEVFLPNADRLWDASKYAAKLENLSKVIATGADML"
     gene            complement(329..1567)
                     /gene="odhB"
                     /locus_tag="PLB46_03455"
     CDS             complement(329..1567)
                     /gene="odhB"
                     /locus_tag="PLB46_03455"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRF75602.1"
                     /translation="MIIELKVPTIGESINEVTLSKWLVKDGDVVALDQSLCEFESDKA
                     TLEFPAEKSGKITLKAEEGAELKIGDVIATLDTSVSIEAAPKKTEAPVVEKQEVKKEI
                     TPAVTSDSSGTSEKQITPLAENIAKENNIDINKITGTGAGGRITKDDVLQFLQQGVGG
                     KEIVAALKRGSRNQREEKVSKLRKTIAKRLVGVKNETAMLTTFNEVDMSAIMAIREKY
                     KVAFEKKQGVGLGFMSFFVKACCNALQQFPAVNAYFNEDVVIYNDYCDISIAVSTPKG
                     LVVPVLRNAESMSMAGVEAKIKELAVKGRDGTLSLEEMQGGTFTITNGGVFGSLMSTP
                     IINAPQSAILGMHKIQDRPIALNGQVVIRPMMYLALSYDHRIIDGKESVSFLVRVKEQ
                     LEKPEQLLFGLDVTELLLEL"
     gene            complement(1626..3155)
                     /locus_tag="PLB46_03460"
     CDS             complement(1626..3155)
                     /locus_tag="PLB46_03460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF75603.1"
                     /translation="MSKQISENISITKTRYVNGILLIICLAGLWLRLIPVHHSLWFDE
                     LATSWVVSDGINQVWERSLFNNVSPFYFYIEYISVQLFGFNEFGLRLPSLIAGMIMIL
                     LVFKIIQLLTQSNISALIAALLVALDAKMIYYSIEVRSYAIATLLVLIMVFYFIKTYH
                     QNYNVKQVVVFGLLIGCLIWAHYLAAIFAICFFAVICFKIFKQNGFNKYAFLLAGALA
                     IVPVLMTLPVIEQFMYVNNNKELFGSYIQPPGLDDIFTTIPHLKYYLLLPLSLFVLTW
                     VVNSIRKQNIAPNKLPQVLLVMFTLLPIGITFLLAFSGTLQIFIIRYIAWFLPLIILL
                     SVYFVAAVPNRYLQVLSLGLLLLFSQVYFSDAIIPKLLNTDFKKELTILLTPEQNTND
                     WKEVVQLVNDTATNPDKIYVKAGLIEAKSIDFNATIDPVLKAYLLSPVNSLYALKPEL
                     ADKATPFYDVKKIAPQEGNYWVIGKLMDWDARYIAAKGQVFTLPDCNIQIYFVPAVNT
                     N"
     gene            complement(3179..5908)
                     /locus_tag="PLB46_03465"
     CDS             complement(3179..5908)
                     /locus_tag="PLB46_03465"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRF75604.1"
                     /translation="MDKFSYIANAEPAYIESLYDTYKANPQAIDTEWKKFFEGFDFAQ
                     SNFSTNGHSENVVTDNKLIDEFKAYNLILAYREKGHLIAKTNPLKERKDRNAQLKLSQ
                     LGFSEADLDKKFGAGIEIGKPNATLKEIIAHLEAAYCQSLGFEYDYIIDPAEKLWMQA
                     KIEGAGKLIQFSPEKKKRILKKLNETVVFEQFLDKKYIGQKRFSLEGGESTIAGLDAI
                     INLGADLGVEEFVFGMAHRGRLNVLANILGKTYGQIFNEFEGNTLPDTTMGDGDVKYH
                     LGYSSQVQTSGDKMVHLKLTPNPSHLEAVNPVMQGFVRAKTDVMYAEDWDKILPVTIH
                     GDAAVAGQGVVYEVLQMSNLKGYRVGGTIHFVINNQIGFTTDFDDARTSNYCTSYAAT
                     VQAPVIHVNGDDAEAVVFASELAVAYRQEFNKDIFVDMVCYRKHGHNEGDDPKYTQPT
                     LYKLIENKKNPREIYVQKLLSSHDIDKELATDMEKDFWNELQQRLDEIKQQPLKYQYQ
                     PPEEAWRKLKKIIDIPVDFAKLPETKISDSQLQIIINNLFKVPEGFVKLKKIEKLFES
                     NKKLIAENKVDWALAELMAYGSILLDGKDVRMSGQDVRRGTFSHRHAVLREENTNEEY
                     IRLNYLAENQGKFRIYNSLLSEFGVLGFEFGYSLASPDHLVIWEAQFGDFNNGAQTII
                     DQFIAASVSKWQRMSGLVMLLPHGYEGQGPEHSSARLERFLQLCAEGNMIVTNITTPA
                     NFFHVMRRQLAFDFRRPLVNMSPKSLLRHPLVQSNIQELSEGKFHELIGDDYAEPAEV
                     KRVILCSGKVYFDLLDYQQKNERTDTAILRLEQLYPFPMTQLNAQLKKYNHAELMWVQ
                     EEPRNMGAWYYIWNLLGTKINKKVSRKSSASPATGFAKIHAKEQNDIVVEAFK"
     gene            6107..6931
                     /locus_tag="PLB46_03470"
     CDS             6107..6931
                     /locus_tag="PLB46_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRF75605.1"
                     /translation="MPSTLAYRDNQYLHKSEIRIGLDDLGFERGFGVFDYCRVRNGKV
                     TFLNDHLERLNHSQSVLQLATPADINIIREVVLKLQQANGLSDAYFKIIISARMVNQQ
                     LQPIITIYQDSWKPYADVMYEKGISLILEEYDKPFSSFKTTFYLGSFRLLPRMHANGS
                     EDVLFYFNDEVRECARCNFFIVKNGLIYTPNENMLLGVTRKHVIQCAKQDQMVIEQSI
                     SVKSLFEADEVFITSTTRNIMPVVKIEDKFIGNGKPGALTLSLMHRFENYCKNYAE"
     gene            6921..7481
                     /locus_tag="PLB46_03475"
     CDS             6921..7481
                     /locus_tag="PLB46_03475"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRF75606.1"
                     /translation="MLSDIQQTLKDNAEADFAEKMKTVAPGAKRIYGVRMPVLNEIAK
                     VAKEGGFELIEQLWEAGAYEERMLAAKTLHKVAKKDPEKAIALVKKYSSEIDNWALCD
                     TLGMQSLKAINKIRGKAIFALANELSTSPIMWQRRLSMVLLEDFCKHPVYHTTIQEMV
                     NRHKGDKEYYIKKAVIWLEASLKKHQ"
     gene            7528..7872
                     /gene="ytxJ"
                     /locus_tag="PLB46_03480"
     CDS             7528..7872
                     /gene="ytxJ"
                     /locus_tag="PLB46_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HRF75607.1"
                     /translation="MNWIELSTEQELLKLTEKSFTQPQAIYKHSTTCGTSFHVKMMLE
                     DEVTPDNIDFHYLDLLANRPLSNKIATQFNVHHESPQILIIKDGKCVYHTSHLAIRME
                     KISQFAKPELSL"
     gene            7967..8671
                     /locus_tag="PLB46_03485"
     CDS             7967..8671
                     /locus_tag="PLB46_03485"
                     /inference="COORDINATES: protein motif:HMM:NF021365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HRF75608.1"
                     /translation="MATSQQINTALLLFTRSQQEEARVKDFSSVHSLRSNSLIAGHLI
                     SHAEKTARSTGLPCFVINSAHQHGNTFGEKLSDAYQQIFKLGFENVIAIGNDCPSLSA
                     QDLRTAAFQLTSKQAVLGPASDGGLYLIGLNKSAFHALSFSALPWETAIVQDMMQVYL
                     SDQAITFILSDVKEDIDFGKQLSSVLAANQISFRLRNAVRSIIASIVQQLSKQFQHPI
                     TINHRIYTSLRAPPVY"
     gene            8766..9104
                     /locus_tag="PLB46_03490"
     CDS             8766..9104
                     /locus_tag="PLB46_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HRF75609.1"
                     /translation="MERTTYYDPKDLKKFGDISKWDAELGKAFFDYYGKVFAEGALTE
                     REKSLIALAVAHAIQCPYCIDAYTQDTMAKGCDEEQMMEAIHVAAAIRGGASLVHGTQ
                     MMNKFKDLTM"
     gene            9150..10208
                     /gene="arsS"
                     /locus_tag="PLB46_03495"
     CDS             9150..10208
                     /gene="arsS"
                     /locus_tag="PLB46_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274671344.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HRF75610.1"
                     /translation="MSSKKSLHALHHKLADNDYQLKVLETHEISTVDFERFDDKLKSI
                     GLFPLKPTNTEIFQVNVGYMCNQTCAHCHVDAGPDRKEIMTRETMQQCLDALAKTSIP
                     TVDLTGGAPEMNPDFRWFVEEITKLGRKTMVRCNLTIIMANKKYHDLPQFFADHKIEV
                     VSSLPFYNKSRTDAQRGDGVFEDSIKALQMLNEVGYGKPGSGLILNLVYNPSGAFLPG
                     SQASLEAQFKKELKRLYQIDFNSLFCITNMPISRFLEYLIDSGNYEEYMQKLIEAFNP
                     IAAQNVMCRNTVSIGWDGNIYDCDFNQMLGLKVNHGAPNHISNWDLELLNNREIVLNQ
                     HCFGCTAGSGSSCGGSVA"
     gene            10227..10835
                     /locus_tag="PLB46_03500"
     CDS             10227..10835
                     /locus_tag="PLB46_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRF75611.1"
                     /translation="MQHKPALIIMLKNPVLGKVKTRLAADIGDERALKIYQELLQHTL
                     AVSKNIQADKFIFYSDVVERSDMFDNSAYKKYVQCSGDLGVRMDYAFSIPFKNEYHHV
                     VMIGADCYDITQQHIEQAFEALANNNDFVIGPANDGGYYLIGMKKWNRWVLENKNWST
                     ATLFDETKSDILNRNGKLFELPTLSDIDTIADIQSHLQLQNL"
     gene            10832..>11714
                     /locus_tag="PLB46_03505"
     CDS             10832..>11714
                     /locus_tag="PLB46_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRF75612.1"
                     /translation="MTEIIIILAACFLAFSNGANDNFKGVSTLFGSGTTNFKSALNWG
                     TITTLAGCIFAVFIAQGLMKNFSGKGLIPDEALKDPSLAIAVALGAASTVFLATKIGI
                     PISTTHAIVGALVGAGFVSVGNALNFNQLGEVFLKPLLLSPFIACASAIILYIIFRQI
                     RLLLKIDKQTHLVVDAPKTDSNNIMPPGISLSNNYRESYNGKIVGFSAQKILDGLHYI
                     SAGAVGFARGMNDAPKIAGLLLLVNFLSVPWMLIMIAVIMAVGGLLNSKKVAQTMSKK
                     ITYMNPGQGFTSNLITAI"
BASE COUNT         3765 a         2205 c         2040 g         3704 t
ORIGIN      
        1 aagcatatcg gcgccagttg caattacttt cgataaattt tccaatttcg ctgcgtattt
       61 gctcgcatcc cagagtctgt ctgcatttgg tagaaatact tcatcgtctt ttgccggatc
      121 gttgatggta tcatacaaat tttcgaggtt gtaaaacatt actccgtatc ctgtagacgc
      181 aactgaatta tcagattgag cgctacatga taaattaaaa aagataaaca agagaaataa
      241 tgattgcaac ttcataaaat aaattaacag attaaaaata aattaaatca ataaacaaac
      301 tagtgccgca ccttaaattc atgttgaatt ataattccaa taacaattca gttacatcta
      361 agccaaatag caattgttcc ggtttttcta attgttcttt cactcgcacc aaaaaactca
      421 ccgattcttt accatcaatt atacggtggt cgtaacttaa tgctaaatac atcattgggc
      481 gaattacaac ttgtccgttc aaagcaattg gccggtcctg aattttatgc atacctaaaa
      541 ttgcactttg aggtgcgtta attattggag tgctcattaa cgaaccaaat acaccaccgt
      601 ttgtaattgt aaaagtaccg ccttgcattt cttccaacga taacgtgcca tctctccctt
      661 taacagcaag ttctttaatt ttggcttcta caccagccat gctcatactt tcagcattac
      721 gcaaaacagg tactacaaga ccttttggtg tagaaacggc aattgaaata tcacaataat
      781 cgttatagat aactacatct tcattaaaat acgcattcac tgccggaaac tgctgcaatg
      841 cattacaaca cgctttaaca aaaaagctca taaagcccaa tcctacaccc tgctttttct
      901 caaatgccac tttatatttt tcgcgaatag ccataattgc actcatatct acttcattaa
      961 aagtagtgag catagcagtt tcgtttttaa caccaactaa acgtttggca attgttttgc
     1021 gcagtttcga tactttttct tcgcgttgat ttcttgaacc gcgttttaaa gcagcaacaa
     1081 tttcttttcc tcccacacct tgttgcaaaa attgcaaaac atcatcttta gtaattcgtc
     1141 cacctgcacc agttccggta attttattaa tatcgatatt attttctttt gcgatatttt
     1201 ctgctaatgg agttatttgt ttttcagatg tacctgaaga atcggatgta actgcaggag
     1261 ttatttcttt tttaacttct tgtttttcaa caacaggagc ttcagttttt ttaggcgcgg
     1321 cttcaatgga tacagatgta tctagtgtgg caattacatc accaattttc aattcagcac
     1381 cttcttctgc ctttaaagta atttttcccg acttttcagc agggaattcc agtgtagctt
     1441 tatcgctttc aaattcgcat aacgattgat ccagtgcaac tacgtcccca tctttcacca
     1501 accacttgga taaagttacc tcgtttatag attcgcctat tgtcggcact tttaattcga
     1561 taatcatagt acaaagttaa acatataata tttgtgaatg caacacgctg taagtcaacc
     1621 gtgacttagt ttgtattgac agcaggcaca aaataaattt gaatattaca atcaggcagc
     1681 gtaaacacct gacctttagc tgcgatatac cttgcgtccc aatccattaa cttgccaatt
     1741 acccaataat tgccttcctg aggtgctatt tttttaacat cataaaaagg ggtggctttg
     1801 tccgccaatt caggcttcaa tgcgtataaa ctatttaccg gtgataacaa ataggccttc
     1861 aaaaccgggt caatagttgc gttaaagtca attgatttcg cttcaattaa gcctgctttt
     1921 acataaattt tatccggatt agtagctgtg tcatttacta actgcactac ctccttccag
     1981 tcgttggtgt tttgttccgg tgtaagtaaa attgttagtt cctttttaaa atcagtattc
     2041 agcaatttag gaataatggc atctgaaaaa tatacttgag agaataaaag caacaaacct
     2101 aaacttagca cttgcaaata tcgatttgga acagccgcaa caaaataaac ggacaataaa
     2161 ataattaagg gcaaaaacca tgcaatatat cgtattataa atatttgtaa tgtacctgag
     2221 aaagcaagta aaaaagtaat gcctattggc aataaggtaa acattacaag caacacttgt
     2281 ggtaatttat tgggagctat attttgtttt ctaatgctat taacaaccca agttaaaaca
     2341 aacaagctta atggtaaaag taaataatat tttaaatgcg ggatggttgt aaaaatatca
     2401 tcaagcccag gtggttgaat atagctgcca aacaattcct tattattatt cacgtacatg
     2461 aattgctcaa ttacaggcaa tgtcattaaa acaggaacta ttgcaagcgc gcctgccaat
     2521 aaaaatgcat atttattaaa tccattttgc ttaaatattt taaaacaaat tacagcaaaa
     2581 aaacaaatag caaaaatggc agcaagataa tgtgcccaga ttaaacatcc tattaaaagt
     2641 ccaaaaacaa caacctgttt aacattataa ttttggtgat atgttttaat aaagtaaaac
     2701 accattatta aaactaacaa agttgcaatt gcataagaac gcacttctat cgaataataa
     2761 atcattttag catccaaagc aactaataaa gcagctatta atgcactgat attcgactgt
     2821 gtaagcaatt gaattatctt aaataccaat aatatcataa tcatgccggc gattaaagat
     2881 ggcaatcgca aaccaaattc attaaatcca aacagttgaa ctgaaatgta ttctatataa
     2941 aaataaaatg ggctgacatt attgaataaa cttctttccc aaacctggtt aattccatca
     3001 ctaacaaccc atgatgtagc taattcgtcg aaccaaagtg agtggtgaac aggtatcaac
     3061 cgcaaccaca atcctgctaa acaaataatc aatagtatgc cattaacata ccttgtttta
     3121 gtaattgaaa tattttcgga aatttgtttt gacatggctt tcaatttcct aaaaaatatt
     3181 atttgaatgc ctctactacg atgtcgtttt gttctttggc atgaattttt gcaaagcctg
     3241 ttgcaggtga cgcactggat tttctgctca ctttcttatt tatcttagtt cctaataaat
     3301 tccagatata ataccaagca cccatattac gcggttcttc ctgaacccac attaactcag
     3361 catgattata ttttttcaat tgcgcgttta attgcgtcat tggaaatgga tacaattgtt
     3421 ccaaacgcaa aatagcagtg tcggttcgtt catttttttg ctgataatcc aacaaatcga
     3481 aatatacttt accgctgcat aaaataacgc gttttacttc cgctggctca gcataatcat
     3541 cgccaattaa ttcatgaaat ttaccttccg ataattcctg aatatttgat tgcaccaaag
     3601 gatgacgcaa taaacttttc gggctcatat taaccaatgg tcttctgaaa tcgaaagcga
     3661 gctgacggcg cattacatga aagaaatttg caggtgttgt aatattcgtt acaatcatat
     3721 taccttcagc acataactgt aaaaagcgct ccagccttgc gcttgagtgt tcagggccct
     3781 gaccttcata accatgtgga agtaacatta ctaatccact catccgctgc catttactta
     3841 cacttgcagc aataaactgg tcaataattg tttgtgcgcc attattaaaa tcaccaaatt
     3901 gtgcttccca aattacaagg tggtcgggtg aagctaaact atatccgaat tcaaatccta
     3961 aaacaccaaa ttcgctcagt aatgaattat aaatcctgaa tttaccttga ttttctgcaa
     4021 gataatttaa gcgaatatat tcttcgttgg tattttcttc acgcaataca gcatggcgat
     4081 gactaaatgt tcctctgcga acatcctgac cactcatgcg tacatctttt ccatccaaca
     4141 aaattgaacc ataagccatt aattcagcta atgcccagtc aactttattt tcagcaatta
     4201 attttttatt agattcaaat aacttttcaa tttttttcaa cttcacaaat ccttccggca
     4261 ctttaaacaa attgtttatt atgatttgta actgactatc cgaaattttt gtttcgggta
     4321 attttgcaaa gtccacgggt atatcaatta tctttttcag ttttctccat gcttcttcag
     4381 gtggctgata ttgatatttt aaaggttgtt gttttatttc atcaagtcgc tgctgtaatt
     4441 cattccagaa atctttttcc atatcagtag ccagttcttt gtcaatatca tggctggata
     4501 ataatttctg cacataaatt tcgcgcggat tttttttgtt ttcaattaac ttgtataagg
     4561 taggttgcgt atacttagga tcatcgcctt cgttgtgacc gtgtttgcga taacaaacca
     4621 tatcgacaaa aatatcttta ttaaattcct gtctatatgc tactgctagt tcactggcaa
     4681 acacaactgc ttcggcatca tctccattaa catgtataac aggcgcttga acagttgctg
     4741 cataacttgt gcaataatta ctggttcggg catcatcaaa atcagtagta aatccaattt
     4801 gattattaat aacaaaatga atggttccac ccacacgata tcctttcaga ttgctcattt
     4861 gcagcacttc atacaccaca ccttgcccgg caactgcagc atcaccatga atggttaccg
     4921 gtaaaatttt atcccaatct tctgcataca ttacatcagt ttttgcgcgc acaaaaccct
     4981 gcattaccgg atttactgct tccaaatggg aaggattagg cgttaatttg aggtgcacca
     5041 ttttatcccc tgaagtttgc acctgagaag aatatcctaa atgatatttt acatcaccat
     5101 ctcccatcgt ggtatcagga agtgtattgc cttcaaattc attaaaaatt tgtccgtatg
     5161 ttttacctaa aatatttgcc aatacattta atcgtccgcg atgtgccatc ccaaaaacaa
     5221 attcctcaac acccaaatct gcacctaaat tgataattgc atctaaaccc gcgatggtag
     5281 attcgccgcc ttctaaagaa aaacgttttt gaccaatata ttttttatct aaaaattgtt
     5341 caaaaacaac tgtctcattt agttttttga gaatacgttt tttcttttcc ggcgaaaact
     5401 gaatcaattt accagcgcct tcaatttttg cctgcatcca taatttttca gcagggtcaa
     5461 taatataatc gtattcaaaa cccaaacttt ggcaatatgc cgcttccaaa tgagctatta
     5521 tttcttttaa agttgcgttg ggtttgccaa tttcaatacc ggccccgaat tttttatcta
     5581 aatcggcttc ggaaaatccg agctgcgaca atttcagttg tgcatttcgg tcttttcttt
     5641 cctttagtgg gtttgtttta gcaataaggt gccctttttc gcgataggcc aaaatcagat
     5701 tgtaagcctt aaattcatct ataagcttat tatcagtcac tacattttcg gaatgcccgt
     5761 tggtagaaaa attggattga gcgaaatcaa atccctcaaa aaatttcttc cattctgtat
     5821 caattgcctg aggattggcc ttgtaggtat cataaagcga ctcaatataa gccggttctg
     5881 catttgcgat ataagaaaac ttgtccatgc gctgctattc tttgcctgtt taacaaagta
     5941 ttttacactt tggtttggaa gggcaaatat cggggttttt gaggggaaaa cagggaaata
     6001 atggtgaaaa ttacttccgg cttaattatt atgttcgttc aaccagctca agtctcaaaa
     6061 gggtttgcga ataccgtaaa tcgttttaat ccagtacttt tgtgttatgc catcaactct
     6121 agcctatcgc gacaaccaat atctccacaa atcggaaatt cgtattggat tagacgacct
     6181 gggttttgaa cgcgggtttg gtgtttttga ctattgccgg gtgcgcaacg gaaaagttac
     6241 gtttttaaat gaccatctgg agcgattaaa tcactcgcaa tcggtattac aacttgctac
     6301 accagccgac attaatatta ttcgtgaagt tgttttgaaa ttgcaacagg caaatggatt
     6361 aagtgatgcc tattttaaaa ttatcatctc ggcaaggatg gtgaatcaac aattacaacc
     6421 cattattact atttatcagg acagctggaa accgtatgct gatgtgatgt atgagaaagg
     6481 aatatcacta attttagaag aatatgacaa gccattttca tcgtttaaaa ctacttttta
     6541 tttaggttct ttccggttat taccgcgcat gcatgctaac ggtagtgaag atgtattatt
     6601 ctattttaat gatgaagtga gagaatgtgc aagatgtaat ttttttattg taaaaaacgg
     6661 acttatttat acgccgaacg aaaatatgtt attgggtgta acgcgaaaac atgtaattca
     6721 atgtgcgaaa caagatcaaa tggtaattga acaatctatt tctgtaaaat cactttttga
     6781 agctgatgaa gtatttatta ccagcactac tagaaatata atgcctgttg taaaaattga
     6841 agataaattt attggaaacg ggaaacctgg tgcattaacc ttatcgctta tgcataggtt
     6901 tgaaaattat tgtaaaaatt atgctgagtg atattcaaca aacattaaaa gataatgctg
     6961 aagctgactt tgctgaaaaa atgaagactg ttgctccggg cgcaaaacgc atatatggtg
     7021 tgcgtatgcc ggtgttgaat gaaatcgcca aagtagccaa ggagggaggg tttgagctga
     7081 ttgaacaatt gtgggaagca ggtgcctatg aagaaagaat gttagctgca aaaaccttgc
     7141 ataaagttgc taaaaaagac cctgaaaagg cgatagcatt ggtgaaaaaa tattccagtg
     7201 aaattgacaa ttgggcattg tgtgatacgc ttggaatgca aagtttaaaa gcaattaata
     7261 aaattcgcgg caaagcaatt tttgcacttg ccaatgaatt atctacttca ccaataatgt
     7321 ggcaacgcag attgtcgatg gtattattgg aagatttttg caaacatcct gtttatcata
     7381 ccaccattca ggaaatggta aacaggcata aaggtgataa agaatattac attaaaaagg
     7441 cagttatttg gctcgaagca agtttaaaaa aacaccaata attgaactat ttccattttg
     7501 ataacattaa cacactaaat taacaacatg aattggattg aattaagtac ggaacaggaa
     7561 ctacttaaac ttacagaaaa atcttttacg cagccacaag ccatatataa gcatagtaca
     7621 acctgtggaa caagttttca cgttaaaatg atgcttgaag acgaggttac tccggataat
     7681 attgattttc actatctaga cttactggct aacagaccat tatccaataa aattgctact
     7741 caatttaatg tgcatcatga atcgccacaa attttgatta ttaaggacgg aaaatgtgtt
     7801 tatcacacct cgcatttggc aatcaggatg gaaaaaatta gtcagtttgc caagccggaa
     7861 ctgagcctct aggtccgatt tctttaaaca tttgttaaaa tctcccggtt aacgaatagt
     7921 tttctatttt agccgaacca aatcaggcat atttggttaa agacacatgg ctactagtca
     7981 acaaatcaat accgcacttt tgctttttac gcgctctcaa caggaagagg cacgtgtaaa
     8041 agacttttcg tcggtgcata gcctgcgttc aaacagtctg attgcaggac atttgattag
     8101 ccacgccgaa aaaaccgcca gaagtaccgg attaccctgt tttgtaatca attctgctca
     8161 ccagcacggc aacacctttg gcgaaaaatt gagtgatgcc taccaacaaa tatttaagct
     8221 gggttttgaa aacgtaattg ccatcggaaa cgattgccct tcgctttctg cacaggattt
     8281 gcgaactgct gctttccagc taacttcaaa acaggccgtt ttaggacccg caagcgacgg
     8341 tgggttatac cttataggtt taaataaaag tgcattccac gccttatctt tttccgcttt
     8401 accgtgggaa acagctattg tgcaggatat gatgcaggta tatttatctg atcaggccat
     8461 tacttttatt ttatctgatg taaaagagga tattgatttt ggcaaacagc tcagttcagt
     8521 tttggctgct aaccaaatta gttttcgatt acgcaatgcc gtacgcagta taattgcatc
     8581 aattgttcag caattgagca aacaattcca acatccaatt acgataaatc atcgtatata
     8641 cacttcattg cgggcgccgc cggtttatta atcgtattta ctcattttta tttacatgtc
     8701 acctgcaaca gcaagtggtg tgttatttta catttactaa tttatcatta agcaaacata
     8761 aaacaatgga aagaactacc tattacgacc cgaaagacct taaaaaattt ggtgatatct
     8821 ctaaatggga tgctgaatta ggaaaagcat tttttgatta ttatggaaaa gtttttgcag
     8881 aaggcgcatt aactgaacgt gaaaaaagtt taatagccct ggctgttgct catgctattc
     8941 aatgtccata ttgcattgat gcatacacac aagacacaat ggctaaagga tgtgatgagg
     9001 aacaaatgat ggaagctata catgttgcag ctgcaattcg cggtggagca tcattagtgc
     9061 atgggacaca aatgatgaat aaattcaaag acctaaccat gtaacgttag ttaaattttc
     9121 aggcatattt ttttaattat tcgaaacaaa tgagttcaaa aaaatcatta cacgctttac
     9181 atcacaaatt agctgataac gattatcagt taaaagtgct cgaaacacat gaaatttcta
     9241 cggtagattt tgagcgcttt gatgacaagt tgaaaagcat cggattgttt ccacttaaac
     9301 caaccaatac cgaaattttt caggtaaatg ttggttatat gtgtaatcaa acctgtgcac
     9361 attgtcacgt tgatgcaggt cccgacagaa aagaaattat gaccagggaa acaatgcaac
     9421 aatgtctgga tgcattggca aaaacaagca ttccaactgt agatttgact ggtggtgcgc
     9481 cggaaatgaa tcctgacttc agatggtttg ttgaagaaat taccaagttg ggcagaaaaa
     9541 caatggtgag atgtaatttg accatcatta tggcaaataa aaaataccat gacctgccac
     9601 aattttttgc cgaccataaa atagaagttg tttcttcctt gccattttat aataaatcgc
     9661 gcaccgatgc tcaacgtggt gatggtgttt ttgaagatag tatcaaagca cttcaaatgt
     9721 tgaatgaagt tgggtatggt aaacccggaa gtggtttaat cttaaacctg gtttacaatc
     9781 cgagtggagc atttttaccc ggcagtcagg cttcacttga agcgcagttt aaaaaagaac
     9841 tgaaaagatt atatcaaatc gatttcaata gtttattctg tattaccaat atgcctattt
     9901 ctcgcttctt ggaatattta attgacagcg gtaattacga ggaatacatg caaaaattaa
     9961 ttgaagcatt taatccaata gcagcacaaa atgtaatgtg tcgcaatact gtttctattg
    10021 gttgggatgg caatatttat gattgcgatt ttaaccagat gcttggatta aaagttaatc
    10081 acggtgctcc gaatcatatc agcaactggg atttagaatt attaaataat cgcgaaattg
    10141 tgctcaacca gcactgcttc ggctgcacag cgggcagtgg cagcagttgt ggtggttcag
    10201 ttgcgtaata aaataaacaa ccactcatgc aacataaacc ggcattaatt atcatgttaa
    10261 aaaaccctgt attgggtaaa gtaaaaacgc gtttggcagc agacattggt gacgaacgtg
    10321 cattaaaaat ttatcaggaa ctattacaac atacgcttgc tgtatctaaa aatatccagg
    10381 ccgataaatt tattttttat agcgatgtag ttgaacgctc cgatatgttt gataacagtg
    10441 cttataaaaa atatgtgcag tgcagcggtg atttaggtgt gcgcatggat tatgcatttt
    10501 ctatcccctt taaaaacgaa tatcaccatg ttgttatgat tggtgcagat tgttatgaca
    10561 tcacccaaca acatattgaa caagcttttg aagcgttagc caataacaat gattttgtaa
    10621 ttgggccagc caacgatggt ggttattatt taataggtat gaagaaatgg aaccgttggg
    10681 tgttggaaaa taaaaactgg agtacggcta cattatttga tgaaacaaaa agtgacatct
    10741 taaacagaaa tgggaaattg tttgaactgc caacactttc agatattgac acaatagcag
    10801 atattcaatc acacttacaa ttgcaaaatt tatgacagaa attattatca tattggcagc
    10861 ctgttttctg gcattttcaa atggggcaaa cgataatttt aaaggtgttt cgacattatt
    10921 cggaagtggc acaaccaatt ttaaatctgc gctaaactgg ggaacaataa caaccttggc
    10981 aggatgcatt tttgccgttt ttattgctca gggattaatg aaaaatttct ccggaaaagg
    11041 attaataccc gatgaagcat taaaagaccc ttcgctagca attgccgttg cacttggtgc
    11101 tgcgagtaca gtttttctgg caaccaaaat tggtattcca atttcaacca cacatgcaat
    11161 tgttggcgca ttagttggcg ccggatttgt ttccgttggc aatgcattaa attttaatca
    11221 acttggtgaa gtatttttaa aaccactttt attaagtcct tttattgctt gtgcttctgc
    11281 aattatatta tatatcattt tcagacaaat aaggttgctt ttgaaaatcg acaaacaaac
    11341 acatctcgtg gttgatgctc ctaaaacaga ctcaaataat ataatgccgc cgggaatttc
    11401 attgagcaat aattaccgcg aaagttataa tggcaaaatt gttggcttta gtgcacaaaa
    11461 aatattagac ggattacatt atatcagcgc aggtgctgtt ggttttgcaa gaggtatgaa
    11521 cgatgcacca aaaattgccg gcttattatt gctggttaat tttttatccg ttccgtggat
    11581 gcttattatg attgctgtaa tcatggctgt tggcggtttg ttgaattcga aaaaagttgc
    11641 acaaaccatg agtaaaaaaa tcacctatat gaatccgggg cagggattta cttccaactt
    11701 aattaccgcg atat
//
LOCUS       DAORKW010000048        11687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_104_length_11687_cov_4.957445, whole genome shotgun sequence.
ACCESSION   DAORKW010000048 DAORKW010000000
VERSION     DAORKW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11687
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104_length_11687_cov_4.957445"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PLB46_03510"
     CDS             <1..989
                     /locus_tag="PLB46_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75613.1"
                     /translation="IVNDKEGNTIGRIAAFYKKQPGSDMVKSAGCGFFECIDDQEIAN
                     TLFDAAKDWLQSKGFNAMDGPINFGERDKWWGLLVEGFDAPVYGMNYHHPYYKSLFEQ
                     YGFKTYFNQFTFVLNRSKNMPPIVYRIKQRLEARNKVKFRTADKRNLAKFAEDFRSIY
                     NQAWANHEDFTPMTSAQSEALMREVKPIMDPELFIFAYVDDRPAGFFIGLPDANQILR
                     YAGGNLNLRGMLNVLLHKPFVKLERIYGVIFGVVPEFQNKGVEAGMAAYMHEIVNNSD
                     KYTHIELLWIGDFNKKMLNFVSHIQATQHRTLITYRKLFDDTIPFERCPIIL"
     gene            1045..2112
                     /locus_tag="PLB46_03515"
     CDS             1045..2112
                     /locus_tag="PLB46_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRF75614.1"
                     /translation="MLIGTPEVNAQSDSVSVDSVKKKSKFFPSPVVGYSPETRLYFGA
                     GLVWYLPPSKKYPDTYPSVLKGVFVYTLNKQIESNVSGDSYLRNNLFKLNYSTSYFKF
                     PDSFFGIGNYTKEEDKEKYDYDYFNIYINGQRKVKENIYAGLKTFFEYTKVYNVDTAG
                     FFANDTIPGKEGGINTGIGPWFTFDTRDNIYFPLKGINVDVSAVVHNEVIGSGYNYVD
                     YLLEVSQFNKIGKDDVLGFNFYGQFVPGNPPFNRMAQLGGDKHMRGNFEGRFRDKNYL
                     TLQAEYRITFWKYFGINVFAGIGEVADEFSNFSAKGLKYSYGAGGRLFIVPEDKLSLR
                     VDYGFDGYGNGGLYVTFREAF"
     gene            2170..2922
                     /locus_tag="PLB46_03520"
     CDS             2170..2922
                     /locus_tag="PLB46_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF75615.1"
                     /translation="MKIEFKLFEHLGKFLIMIRRMFSRPARWEMYWRELFRQMNDIGV
                     GSAFIVLLVSIFIGAVTAVQTAYQLYGTPFVPSSYIGLIVRNTIIFELAPTFTALILA
                     GKVGSNLASELGSMRVTEQIDALEIMGVNTESYLIATRIIAAVITIPVLVAIAAYSGI
                     MGGYIATSLSSNYSTEDYVRGIRMLFDPFDVQLMFIKSFTFSFIVSSISCYMGYYVKG
                     GSVEIGKASTQAVVYSCIFVLLADYVIASLLL"
     gene            2919..3695
                     /locus_tag="PLB46_03525"
     CDS             2919..3695
                     /locus_tag="PLB46_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF75616.1"
                     /translation="MIEISNITKSFGEQRVLTDVSTVFEAGKTNLVIGISGSGKTVLM
                     KIMTGLMTPDSGKVHFNGRDFFALSRNDQKNLRREIGMLFQGSALFDSMTVEENVMLP
                     LLMLSNATKKEKLDRVNFCLERVNLAGANKKFPGEISGGMKKRVGIARAIVLNPKYLF
                     CDEPNSGLDPRTSIVIDDLIIEITREFNMTTIINTHDMNSVMEIGEKIIFLYKGVKDW
                     EGDKSQILDVTDENLNSFIFASDFLKDARDALKEKIARSK"
     gene            3771..5135
                     /locus_tag="PLB46_03530"
     CDS             3771..5135
                     /locus_tag="PLB46_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75617.1"
                     /translation="MKIHLLLFLFIFGCTQINAQWTNFCTGNTDGFVVDFARYNNSIY
                     STGFFHTICDADAEFVASWDGSNWNSTDNSFNQEGHAIDSINGLLYASLYQFTTDSNY
                     VYTYNGSTWSTVGNGVFQNNPGPGFPKNATIYDIIEYDGKVVVCGDFDIAGDIVVNGI
                     MQWDGTTWLPLGEGLTEFIPGMPSILYPHNMTTFDDDLIVCGNFNKAGGTIVNGIARW
                     DGTTWHALGEGFNNSAYAVCVYDGELYAGGEFTMSGTTPLGRIAKWNGVSWENPGFEV
                     AYSVTALHEFVHTLKTIDGKLYITGGFDQLIIDGVTTDAGSILAFDGTTIDTLAGGVE
                     GEIEAIIEYGNGFLVAGAFTKAGGLDMENFAIYTNPANAVENTTIKLFEVYPNPAADY
                     LQVNLIYPNATEITISDSQGRCVLTSTIHTSTALPISSLASGCYFITAKSAQTVYSEL
                     IFIE"
     gene            5194..6075
                     /gene="sucD"
                     /locus_tag="PLB46_03535"
     CDS             5194..6075
                     /gene="sucD"
                     /locus_tag="PLB46_03535"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRF75618.1"
                     /translation="MSVLVGKYSRVIVQGFTGKEGTFHAEQMIAYGTNVVGGVTPDKG
                     GSKHLDRPVFNTVEDAVKKEGANTTIIFVPPAFAADAIMEAADAGIKVIVAITEGIPV
                     QDMIKVKSYISNKDCRLIGPNCPGIITPEEAKVGIMPGFIHKKGHIGIVSRSGTLTYE
                     AVDQVTKAGMGQSTCIGIGGDPVPGTTTLDAVQLLMADDETEGIILIGEIGGTMEAHA
                     ANWIKAHGTKPVVGFIAGQTAPKGRTMGHAGAIVGGKDDTAAAKMEIMRNCGIHVCDS
                     PAILGEMMAKAIKGQLV"
     gene            6145..6891
                     /gene="fabG"
                     /locus_tag="PLB46_03540"
     CDS             6145..6891
                     /gene="fabG"
                     /locus_tag="PLB46_03540"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRF75619.1"
                     /translation="MKLLENKTALITGASRGIGEAIALKFAEAGANVAFTYLSSEAKA
                     LALEEKLNSFGIKAKGYKSNAGVYAEAEQLINDVMADFNALDVVVNNAGITKDNLMLR
                     MSEQDWDAVIDVNLKSVFNLTKFAIKPMMKARKGSIINVSSIVGLIGQGGQANYSASK
                     AGIIGFTKSIAKELGSRNIRCNAIAPGFIETDMTGELPEELKQSYFKSIPLGRFGKGS
                     EVADVALFLASDMSSYVTGQVLSVCGALHT"
     gene            7022..9127
                     /locus_tag="PLB46_03545"
     CDS             7022..9127
                     /locus_tag="PLB46_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75620.1"
                     /translation="MNVQLFTDYPSWFWLLCFLAGGIYAFAMYYREKKMAEAISNKWW
                     LRGLAVLRFTGVVILAFLLLNPYLKSKTNETEKPVVVIMQDNSSSIQSAVGDSVGYRE
                     KLNSLYESLSETYDVRTYSFGSSMQEGFDSLNFKEKSTDISSALSDLNNLYENLNVGA
                     VILASDGIYNTGSNPVYVKNNLGAPFYTIALGDTTPKKDLRITRVLHNNIVYLNDKFV
                     VTADIEAIFCNGKSSKGKIIEVLSGSTKTIGEQVFEIKDNNFNTSLTWEVDANTPGIR
                     HFQVSLTSIEGEYTTVNNMQDIFIEVLDARQKVLLLANAPHPDLNAIRQSIESNRNYE
                     IDLQLANELKGNVNDYDIIILHQLPSSKNDITQVLADIKNKQIPTWFFAGSQTATGAF
                     NKAQPLVSISSGGQSANEITGIYQNNFNLFNLEPSTISLFSKLPAINAPYGQYTVSPA
                     SQVLLNQKIGAVNTKNPLLVYSLAGSSKMAVFCAENFWKWRLYDYVMNNNQVATNELV
                     SKTLQYLSTKNDKKQFRVNQTRTVINENEPVILDAELYNDSYELINEPDATLTIKNES
                     GKDFPFQFSKNNKSYVLRAGFFPAGNYTYEGKVVYNGKEFKDNGAFSVSPLRLETINT
                     TADHQLLNQLAVQNGGQMVYPDNMQTLTSLINESAAAKPVMREIVRTQSIINLKWICF
                     VLFGLLGIEWFVRKFNGGY"
     gene            complement(9130..9453)
                     /locus_tag="PLB46_03550"
     CDS             complement(9130..9453)
                     /locus_tag="PLB46_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRF75621.1"
                     /translation="MAKKSTFRQRLKQWTVPSMLLNKYVFTLFVFGVWMTFFDQNNFI
                     RQYNRIHELNTAKGKMSYYIEETQLANEQLVKLKSDMQALEKFAREEYYMKKPNEDVF
                     VILEK"
     gene            complement(9567..10850)
                     /gene="eno"
                     /locus_tag="PLB46_03555"
     CDS             complement(9567..10850)
                     /gene="eno"
                     /locus_tag="PLB46_03555"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRF75622.1"
                     /translation="MSHIINIYARQILDSRGNPTVEAEVTTEFGAIGRAAVPSGASTG
                     VHEAAELRDEDPDVYLGKGVLKAVENVNEIIATELEGMNVYDQNHIDTLMMELDGTPN
                     KSKLGANAILAVSLACAKAAAIELNVPLYRYVGGVNANTLPVPLMNILNGGAHADNKI
                     DFQEFMIAPIGGDNFSDALRMGVEVFHHLKKILKNKGYSTNVGDEGGYAPNIQTNEEA
                     IEIVLKAIESAGYIPGEDIVIAMDAAVSELYDTASGMYIFKKSDGRKLTSAEMVDYWA
                     NWAKKYPIASIEDGCAEDDWAGWKMLTEKIGSKIQLVGDDLFVTNVKRLNKGIKENIA
                     NSILVKVNQIGSLTETIQAVDMAHRAKYTSVMSHRSGETEDTTIADLAVALNCGQIKT
                     GSASRSDRIAKYNQLIRIEEELGASAVWPGNVFMK"
     gene            complement(10896..>11687)
                     /gene="carA"
                     /locus_tag="PLB46_03560"
     CDS             complement(10896..>11687)
                     /gene="carA"
                     /locus_tag="PLB46_03560"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRF75623.1"
                     /translation="QDYLIEERLVGISDVDTRAIVRHIRSKGAMNCIISSETTDIDVL
                     TAKLNEVPDMNGLELASRVSTTTPYYIGNSDAEKKVIVYDFGVKKSILENIAIRGCYL
                     KVVPAKTKFEDTLDFKADGYFISNGPGDPASMGYAVETVKQILQNDKPLFGICLGQQL
                     LGLANGVTTYKMHHGHRGCNHPVKNLITGLSEITTQNHGFSIKEEDMRASKNMEITHI
                     NLNDNTIEGFRLTDKKAFSVQYHPEATPGPHDARYLFDEFIKLMH"
BASE COUNT         3675 a         2032 c         2251 g         3729 t
ORIGIN      
        1 atatcgttaa cgataaagaa ggcaacacca ttggccgtat agcggctttt tacaaaaaac
       61 aaccgggttc cgatatggtg aaaagtgccg gttgtggctt ttttgaatgt atcgatgacc
      121 aggaaattgc caatacctta tttgatgcag ctaaagactg gctgcaaagc aagggtttta
      181 atgcaatgga cggcccaatc aattttggcg aacgtgataa atggtgggga ctgctggtag
      241 aaggctttga tgcccctgtt tacgggatga attaccatca cccctattat aaatcgttgt
      301 ttgagcaata tggttttaaa acctatttta accaattcac ttttgtatta aatcgcagca
      361 aaaacatgcc accgattgtg tacaggataa aacagcgttt agaagccaga aacaaggtaa
      421 aatttcgcac tgccgataaa cgcaatctgg caaaatttgc agaagatttc aggagtatat
      481 ataatcaggc atgggccaat catgaagatt ttacacctat gacttcagca caaagtgaag
      541 ccttgatgcg ggaagtaaaa ccgattatgg acccggaatt attcattttt gcttatgttg
      601 acgaccgacc tgcaggtttt tttatcggct taccggacgc caatcagata ctgagatatg
      661 caggaggtaa tctgaattta agaggtatgt taaatgtgtt attacacaaa ccttttgtga
      721 aacttgagcg tatatatggt gtcattttcg gcgttgtgcc tgagtttcaa aataagggtg
      781 tagaagccgg aatggcagct tatatgcatg aaatagtgaa caatagtgat aaatacacac
      841 atattgaact cttatggatt ggcgatttca ataaaaaaat gttgaatttt gtgtcgcaca
      901 tacaggcaac ccaacacaga acgttaatta cctacagaaa actttttgat gataccattc
      961 cgtttgaacg ttgtccgatt attctataaa aacatctttt tttttacgag gtattacaaa
     1021 gtggcggttt taataattgc cattatgctt ataggcacac cggaagttaa tgcacaatcg
     1081 gatagtgtta gcgtagattc tgttaaaaag aaaagtaaat ttttcccgtc tcctgttgtt
     1141 ggatactcac ctgaaacacg tttatatttc ggcgcaggat tagtttggta tttaccacca
     1201 tcaaaaaaat atcccgacac ctacccttct gtattaaaag gcgtttttgt ttatacatta
     1261 aacaaacaaa ttgaaagtaa tgtaagcggc gatagttacc tgcgcaataa tttattcaaa
     1321 ctaaattatt ctacatctta ctttaaattt cctgactcat tttttggcat tggcaattac
     1381 accaaagaag aggataaaga aaaatacgat tatgattatt tcaatattta cataaacgga
     1441 caacgaaaag taaaagaaaa tatttatgcc ggtttaaaaa cattttttga atacactaaa
     1501 gtgtataatg tagatactgc cggttttttc gcaaatgata ctatacccgg gaaggaaggt
     1561 ggcattaata ccggtatagg accatggttt acatttgata caagagataa tatttatttt
     1621 ccgttaaaag ggattaatgt tgacgtttcg gcggtggtgc acaacgaagt aattggcagt
     1681 ggttataatt atgtagatta tttattggag gtaagtcaat ttaataaaat tggcaaggac
     1741 gatgttttag gatttaattt ttacgggcag tttgttccgg gaaatccacc cttcaacaga
     1801 atggcacaat taggaggtga taaacatatg cgtggtaatt ttgaaggtag attcagagat
     1861 aaaaattatt tgacattgca ggcagagtat cgcattacct tttggaaata ttttgggata
     1921 aatgtatttg caggaatagg tgaagttgcc gatgagtttt ccaatttttc ggccaaagga
     1981 cttaaatact cttatggagc cggcggtcgt ttattcatag ttccggagga caaattgagt
     2041 ttacgcgttg actatggttt tgacggttac ggcaatggcg gtttatatgt tacctttaga
     2101 gaagcatttt aattgaataa ttttcatcat aggccgacaa cttaaggcta aaatgataat
     2161 tttacgaaaa tgaaaataga atttaagctg tttgagcatc ttggcaagtt tctgattatg
     2221 atcagacgca tgttttcaag acctgcgcgt tgggaaatgt attggcgtga gttgtttcgc
     2281 caaatgaacg acatcggtgt tggctctgcg tttattgtat tgttagtttc tatttttatt
     2341 ggcgcagtta ctgcagtgca aactgcctat cagttatacg gaacaccgtt tgttccttct
     2401 tcgtacatcg gattaattgt ccgcaatacc attattttcg aattagcccc tacattcacc
     2461 gctttaattc ttgccggtaa agtaggttcc aatttagcat ctgaattagg ttcaatgcgc
     2521 gttaccgaac aaattgacgc attggaaatt atgggtgtaa atacagaaag ttatttaatt
     2581 gccacacgca ttattgctgc tgtaattacc attccggttt tagttgccat agcagcttat
     2641 tcaggaatta tgggtggata tattgctaca tcattgtcga gtaattattc aactgaagat
     2701 tatgtgcgag gaatcagaat gttatttgac ccgtttgatg ttcaattaat gtttatcaaa
     2761 tcatttactt tttcatttat tgtttcatct atttcctgtt atatgggata ttatgtaaaa
     2821 ggtggttcag tagaaattgg aaaggccagt acgcaggcgg tagtatatag ttgtattttt
     2881 gttttattag ctgattatgt tattgcaagt ttattgttat gattgaaata tccaatatca
     2941 caaaatcgtt tggcgaacag cgtgtattaa ccgatgtttc aactgtattt gaagcaggca
     3001 aaactaattt agtaattggt atcagcggct cgggcaaaac agttttaatg aaaataatga
     3061 ccggtttaat gacacctgat tcaggtaaag ttcattttaa cgggcgcgac ttttttgcac
     3121 tcagccgcaa cgatcaaaaa aatctccgcc gtgaaatcgg catgttattt caaggctcgg
     3181 cattattcga ttccatgact gtggaagaaa atgtgatgtt gccattgctg atgctcagca
     3241 atgccactaa aaaggaaaaa ttagaccgcg taaatttttg tctcgaaaga gtaaatcttg
     3301 ccggagcaaa taaaaaattc ccgggcgaaa tatcaggtgg tatgaaaaaa cgtgttggta
     3361 ttgctcgtgc aattgtgctc aaccctaaat atttattttg cgatgaaccc aactcaggat
     3421 tagacccacg tacatcaatt gtaatcgacg atttaattat tgaaattaca cgtgagttta
     3481 atatgactac tatcattaat acacacgaca tgaatagtgt gatggaaatt ggtgaaaaaa
     3541 ttatattcct ttacaaggga gtaaaagatt gggaaggtga taaatcacaa atattagatg
     3601 taactgacga aaatctgaac tccttcatat ttgcatctga ctttctgaaa gatgcacgag
     3661 atgcattgaa ggaaaaaatt gccagaagta aataaatcca gcttaatatt ctgacattat
     3721 cgttacacca ttttttgtag ctttaattaa tattacccta aaattgagat atgaaaatac
     3781 atttactgct atttttattc atatttggat gtacccaaat caatgcccaa tggaccaatt
     3841 tttgtaccgg taatacagat ggatttgttg tagattttgc acgatacaat aacagtattt
     3901 actccacagg tttttttcat actatttgcg atgccgatgc cgagtttgtc gccagttggg
     3961 atggcagcaa ctggaattca acagacaatt cttttaatca ggaaggccat gcaattgatt
     4021 ccattaatgg acttttatat gcgtcattgt atcagtttac cactgattca aactatgtgt
     4081 acacatacaa tggcagtacg tggtcaacag ttggcaacgg tgtttttcaa aataatccgg
     4141 gtccgggctt tccaaaaaac gcaactattt acgacatcat tgaatatgat ggtaaagttg
     4201 tagtctgcgg cgattttgat atagcaggtg atatagttgt aaatggcata atgcaatggg
     4261 atggtacaac ttggttacca ttaggtgaag gactcacaga atttattccc ggtatgccat
     4321 ctattttata cccccacaac atgaccactt ttgatgacga tttaattgtt tgtggtaatt
     4381 ttaataaagc aggcggaaca attgtaaatg gtattgcccg atgggatgga acaacatggc
     4441 atgcattagg cgaaggattt aataattcag catatgcagt atgtgtttat gatggcgagt
     4501 tgtatgccgg aggcgaattt actatgagcg gaacaacacc acttggccgc attgcaaaat
     4561 ggaatggcgt gtcatgggaa aaccctggat ttgaagttgc ctattcagtt actgcattac
     4621 atgaatttgt gcatacgtta aaaacaattg atggtaaatt atatattaca ggtggttttg
     4681 atcagttaat tatagatggc gtaacaaccg atgcgggtag tattttagct tttgacggta
     4741 caactatcga taccttagca ggtggcgtag aaggtgaaat tgaagccatt attgaatatg
     4801 gaaatggttt cctggtagca ggcgctttta cgaaagccgg aggtttagat atggaaaatt
     4861 ttgccatcta taccaacccc gctaatgccg ttgaaaacac aacaataaaa ctattcgagg
     4921 tttatccaaa tccggctgca gattatttgc aagtcaactt aatttatccc aatgctactg
     4981 aaatcaccat tagcgacagc cagggtcgtt gtgtgttaac ttctactatt catacttcaa
     5041 cagcccttcc aatatcgtcg ttggcgtcag gttgttattt cataactgca aaaagtgccc
     5101 aaactgttta ttctgaatta atttttatcg aataattgct ttgtgattta agttttggtg
     5161 ccataataaa caaattcatt ttatcttcgc accatgagcg tattagtagg aaaatattcc
     5221 agagtaatcg tacagggatt taccggaaaa gaaggaacct tccatgcaga acaaatgatt
     5281 gcctatggca ctaacgtagt tggtggtgta acaccggata aaggtggcag taagcattta
     5341 gaccgaccgg tgtttaacac ggttgaagat gctgtaaaaa aagaaggcgc taatactacc
     5401 attatatttg ttccacctgc gtttgcggcc gatgccatta tggaagctgc ggatgccgga
     5461 atcaaggtaa ttgtagccat aaccgaagga attccggttc aggatatgat taaagtaaaa
     5521 tcttacatca gcaataaaga ttgtcgttta ataggcccaa actgtccggg tatcataact
     5581 ccggaagaag caaaagtggg cattatgcct ggttttattc ataaaaaagg tcatattggt
     5641 attgtttccc gttcaggaac tttaacttac gaagctgttg accaggtaac taaagccggc
     5701 atgggtcaaa gcacatgtat cggtattggt ggcgaccctg ttccaggcac aactacatta
     5761 gatgctgttc aattattaat ggccgacgat gaaaccgaag gtattattct tatcggtgaa
     5821 attggtggta ctatggaagc acacgctgcc aattggatta aagctcatgg tactaaacct
     5881 gttgtaggtt tcattgcagg tcaaaccgca ccaaaaggac gcactatggg tcacgccggt
     5941 gctattgttg gtggtaaaga tgatactgct gctgccaaaa tggaaattat gcgcaattgc
     6001 ggaatccatg tatgtgacag cccagcaatt ttaggcgaaa tgatggcaaa ggcaattaaa
     6061 ggccagctcg tttaattttt tgaaacagta aacccttaca acatcctttg ggtgattgta
     6121 ttacatattt gaaataaccg catcatgaaa ttacttgaaa acaaaactgc cctaatcaca
     6181 ggcgcttcca gaggaattgg cgaagctatt gcactcaaat ttgctgaagc aggagctaat
     6241 gttgctttca cttatttaag cagcgaagca aaagcattag cactggaaga aaaactgaat
     6301 agttttggta ttaaagccaa agggtataaa agtaatgcag gtgtttatgc agaagctgaa
     6361 cagctgatta atgatgtaat ggctgatttc aatgcacttg atgttgttgt gaataatgcc
     6421 ggtattacta aagacaattt gatgttgcgc atgagtgaac aggattggga tgccgtaata
     6481 gatgtgaatt taaaatcggt tttcaacctg acaaaatttg ccattaagcc aatgatgaag
     6541 gcgcgcaaag gcagcatcat taatgtttcg tctattgtag gattaattgg tcaaggtggt
     6601 caagctaatt attcagcttc taaagcaggg attattggtt tcactaaatc aatagcaaaa
     6661 gaattaggct ccagaaatat ccgttgtaat gctattgcac cgggctttat tgaaacagat
     6721 atgacaggtg aactgcctga agaattaaaa caatcttatt tcaaatctat accactgggt
     6781 cgttttggaa aaggaagtga agtagctgat gtagcattgt ttttagccag cgatatgagt
     6841 agttatgtta ccggacaagt attgagtgtt tgcggtgcgt tacatacctg atttttactt
     6901 ttcttgctca tattcaaata gtagctgttc ccacatttgt atttggtaac gaaatttaac
     6961 tgttatccta aatcagtttt actaccgctt actcgtttag tcattgtata ttcgcaacgg
     7021 catgaatgtt caattattta ctgattatcc atcctggttt tggctcctgt gttttttagc
     7081 cggaggtata tatgcttttg ctatgtacta ccgcgagaaa aaaatggcag aggcaatcag
     7141 taataaatgg tggttgcgtg gattggcggt attacgtttt accggagtgg ttattttggc
     7201 ttttttgtta ttaaatccgt atttaaaatc aaaaaccaat gaaaccgaaa aaccggttgt
     7261 tgtaataatg caggataatt catccagtat acaatcagct gttggcgaca gtgttggcta
     7321 tcgcgaaaaa ttaaacagtt tatatgaatc actttccgaa acgtatgatg taagaacata
     7381 ttcgtttggc agcagtatgc aggaaggatt cgattcttta aattttaaag aaaaatcaac
     7441 agacatttct tcggcactta gcgatttaaa taatttatat gaaaatttaa atgttggtgc
     7501 tgtaattctg gccagcgatg gaatttacaa taccggaagc aaccctgtat atgtaaaaaa
     7561 taatttaggt gctccatttt atactattgc attaggagac acaaccccta aaaaagattt
     7621 acgaattaca cgcgtgttac acaataatat tgtttatttg aacgataagt ttgttgtaac
     7681 tgccgatata gaagctatat tttgtaacgg caaatcttct aaaggaaaaa ttatagaagt
     7741 gttatccggc tcaacaaaaa caattggcga gcaggttttt gaaatcaagg ataataattt
     7801 caataccagt ttaacatggg aagtagatgc caatacaccc ggaattagac attttcaagt
     7861 gagtttaaca tctattgaag gagaatatac aacggtaaat aacatgcagg atatatttat
     7921 cgaagtatta gatgccagac aaaaagtttt attattagca aatgcaccac atccagattt
     7981 aaatgctata cgtcaatcta ttgagtcgaa ccgcaattat gaaattgacc ttcagttagc
     8041 aaatgaatta aaaggtaatg tgaatgatta cgatatcatt attttacatc agcttccatc
     8101 ttcaaaaaat gatattacac aagttttagc cgacatcaag aataaacaaa ttccaacctg
     8161 gttttttgca ggcagtcaaa cagcgacagg tgcatttaac aaagcacaac cattagtatc
     8221 tattagttca ggaggccaat cggcaaatga aataaccggt atttatcaaa acaattttaa
     8281 cttatttaat ctggagccaa gcactattag tttgttttct aaattacctg ctataaatgc
     8341 accatatggt caatataccg tttcaccggc ttcacaggta ttattaaatc aaaaaatagg
     8401 tgcagtaaat acaaaaaatc cgttacttgt ttacagcttg gcaggtagtt caaaaatggc
     8461 tgttttttgt gcagagaatt tttggaaatg gcgtttgtat gattatgtaa tgaataacaa
     8521 tcaggttgct accaacgaat tggttagcaa aacacttcaa tatctttcta caaaaaacga
     8581 caaaaaacaa tttagagtaa atcaaacgcg tacggtaata aatgaaaatg aaccggtaat
     8641 tcttgatgct gaattgtata acgatagtta tgaattaatc aatgaaccgg atgccacact
     8701 tactataaaa aatgagtcgg gaaaagattt tccatttcaa ttttcaaaaa ataataagag
     8761 ttatgtttta agagcaggtt ttttccctgc agggaattac acctatgaag gcaaagtggt
     8821 gtataacggc aaagaattta aagacaatgg tgcatttagt gtatcaccat tgcgattaga
     8881 aacaattaat actacagctg accatcaatt acttaatcaa ttagctgtac aaaacggtgg
     8941 tcaaatggtg tatcctgata acatgcaaac acttacttca ctcatcaatg aaagtgcagc
     9001 agccaaaccg gtgatgcgtg aaattgtaag aacccaatcc atcattaatt taaaatggat
     9061 ttgttttgtc ctttttggtt tgttgggaat agaatggttt gttcgaaaat ttaacggcgg
     9121 ctattaatat tacttctcta aaataacgaa tacatcttcg ttcggttttt tcatgtaata
     9181 ttcttcacgg gcaaattttt ccaatgcctg catgtctgac ttgagtttaa ctaattgttc
     9241 gttagctaat tgtgtttctt ctatgtagta cgacatttta ccttttgccg tatttaactc
     9301 gtgtattcta ttgtattgtc tgataaaatt gttctggtca aaaaatgtca tccaaacgcc
     9361 aaaaacaaat agggtaaata catacttatt gagcaacatg gatggcactg tccattgttt
     9421 taatctttgt ctgaatgttg atttttttgc catgatatat tggtaaagat acagtgcacc
     9481 tgatgaatca gtaacgtcta aaaaagaaaa aaccgtgtgg atagtttaca aaacagtcca
     9541 cacggcacaa attttaacgt ttcagcttat ttcatgaaca cgttgcccgg ccaaaccgct
     9601 gaagcaccta actcctcttc aatacggatg agctggttat attttgcaat cctgtcgctt
     9661 cttgaagcag aaccggtttt aatttgtccg cagtttaatg ctacggctaa atctgcaatg
     9721 gtagtatctt ctgtttcacc acttctatgg ctcattactg atgtatattt tgctctgtgc
     9781 gccatatcaa cagcttggat agtttcggtt aaagaaccaa tctgatttac tttaaccaga
     9841 atagaattgg caatattttc tttaatccct ttatttaaac gttttacatt agttacaaac
     9901 aaatcgtcgc caaccaattg gattttactg ccaatttttt ctgtcagcat tttccaacct
     9961 gcccaatcat cttcagcgca tccatcttca attgaagcaa taggatattt ttttgcccaa
    10021 ttagcccaat aatctaccat ttcggcagat gttaattttc tgccatctga ttttttgaaa
    10081 atgtacatac ctgaagcagt atcgtataat tcacttaccg ctgcatccat ggcaataact
    10141 atatcttcac ccggaatata tcctgcgctt tcaattgcct ttaatacaat ttcaatagct
    10201 tcctcattgg tttgaatatt tggtgcataa ccaccttcat cgccaacatt ggtagagtaa
    10261 cctttatttt ttaatatttt tttcaggtga tgaaatactt caacacccat gcgtaatgca
    10321 tcagaaaaat tatcacctcc aatcggagca atcataaatt cctggaaatc aattttatta
    10381 tccgcatgtg caccaccatt taaaatattc attaatggca caggaagtgt gtttgcatta
    10441 acaccaccaa catagcgata taaaggaaca tttaattcaa ttgcagctgc tttagcacat
    10501 gccaaagaaa cagcaagaat agcattcgct cctaatttag atttatttgg tgtaccatcc
    10561 agttccatca tcagggtatc gatatgattt tggtcatata cattcatacc ttctaattct
    10621 gtagcgatta tttcgtttac attttcaact gcttttaata caccttttcc taaatacaca
    10681 tcagggtctt catcgcgcag ttcagcagct tcgtgaacac ctgtacttgc acctgaagga
    10741 acagcagcgc gtccaatagc accgaactct gtagtaactt ctgcttcaac agttggatta
    10801 cctcttgaat ctaaaatttg tctggcataa atattaataa tgtgggacat agatataagt
    10861 tatgagtgat aaattaaatg tgaaagtgtt tattgttaat gcattaattt tataaattca
    10921 tcgaataaat atctggcatc atgtggaccc ggtgttgctt ctggatgata ttgcacactg
    10981 aatgcttttt tatcggttaa tctaaatcct tcgatggtat tgtcatttaa attaatatgc
    11041 gttatttcca tatttttaga tgcacgcata tcttcttctt taatactaaa tccgtgattt
    11101 tgggtggtga tttcacttaa accggtaatt aaatttttaa ccggatgatt acaacctcta
    11161 tgtccgtgat gcattttata tgtagtgaca ccatttgcca aacccaataa ttgttgccct
    11221 aaacaaatac caaataaagg cttgtcgttt tgcagtattt gtttaacagt ttcaacagca
    11281 taacccatac ttgcagggtc gcctggacca ttcgaaataa aataaccgtc agctttaaaa
    11341 tctaaagtgt cttcaaattt agttttagcc ggtacaactt tcaaataaca tccgcgaatg
    11401 gcaatatttt cgaggatact ttttttaacc ccgaaatcgt acacaattac ttttttctca
    11461 gcatcgctgt tacctatata ataaggtgta gtggtagaaa cgcgactagc caactccaac
    11521 ccgttcatat caggcacttc attgagtttg gctgttaata catctatatc ggtagtttca
    11581 ctactaatta tacagttcat tgcgccttta cttctgatat ggcgaacaat ggcacgcgta
    11641 tccacatctg aaatccccac tagtctttct tcgattaaat aatcctg
//
LOCUS       DAORKW010000049        11639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_107_length_11639_cov_6.058702, whole genome shotgun sequence.
ACCESSION   DAORKW010000049 DAORKW010000000
VERSION     DAORKW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11639
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107_length_11639_cov_6.058702"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLB46_03565"
     CDS             <1..472
                     /locus_tag="PLB46_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75624.1"
                     /translation="NPQSQQKGGQSGMSKELAEMAQKQAMIREALEKMAKELGGGNTE
                     DGKLAKQLQQLADQMDKTEEDIVNKQISIETLKRQEEILTRLLEAEESERKRKQDNER
                     KSNTANEINRPVPPAIEEYLKKRNAELDLYKTVSPELKPFYKNLVEEYLRSISY"
     gene            490..900
                     /locus_tag="PLB46_03570"
     CDS             490..900
                     /locus_tag="PLB46_03570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF75625.1"
                     /translation="MSAETISKQISLKSQPSNIVLIEPFIEEVRMRLNIADEMYGNIL
                     VCVTEAVNNAILHGNKADESKEVVVSIQKEQNKLTCLVKDQGCGFDFNNLPDPTAPEN
                     IENCGGRGIFLMKHLSDLVVFSNDGSNVEIQFRL"
     gene            900..1325
                     /gene="ybeY"
                     /locus_tag="PLB46_03575"
     CDS             900..1325
                     /gene="ybeY"
                     /locus_tag="PLB46_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRF75626.1"
                     /translation="MSAIQFVNKKTRFKLKEQERIASWVERVVLMYHKFPGDIQYIFC
                     DDTFLAEMNQQYLQHNTLTDIITFNYNSGHYISGDIFISIDRIKENAATYKTTIEDEL
                     HRVMIHGILHLCGLNDKTAKDKKLMQEKENEALEILTRV"
     gene            complement(1804..2484)
                     /locus_tag="PLB46_03580"
     CDS             complement(1804..2484)
                     /locus_tag="PLB46_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75627.1"
                     /translation="MKYLSTYLLLFFPLLIMAQAPGKYSKPNDFSHYAISLGTINSPS
                     NDAFQTADLWSGKYYALYGEARISTIEVLFKTAPKNRFHIRDIIDLQLGVGYGSGFYA
                     NAAFAGGLKFKYDINQNMDFGFNVMGRLALDHMSYYGLFAQPFARYKWVMVQYGYGEN
                     NPDNVSDSEERKFNQLNAKIYLNPDELHSRYINFNYWHIQARNNQVTNPWEGNTQLMI
                     GFGWQLGS"
     gene            complement(2565..3680)
                     /locus_tag="PLB46_03585"
     CDS             complement(2565..3680)
                     /locus_tag="PLB46_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75628.1"
                     /translation="MQHQNNTTLVDFEPGTPANKSQFIRQYIVAKLQLSMLMILIIGF
                     TKINAQNSNTLLLKEGMIIESTVYTYKTPLLIDPIKWVKTKDEKKEIAVDKFNADVAA
                     GTIPPATTGSLPLVVNEKSETEEMITFNCTATIGASGGNNIKIQQKGDTVCYLFNNAA
                     FYPIVDAKGDTIGMNYYSAKTFPRNIEIGNIIPGHLSEMNLFPYDFLNTSRRYFNLDG
                     GDGYNYSGFVNVRTSTKMDINTITLYTPYTVIGKEDMVISGKSYTTYKMFNAQWIKTT
                     NNSVVTEIPTVYFDDPVLSKKIKDYFSDLASKKSYQRAKERIAAKIDKFSMTNEYGYT
                     ENLIESWYCPELGMFTLARVYDADGAILVETKVTSIK"
     gene            complement(3729..4385)
                     /locus_tag="PLB46_03590"
     CDS             complement(3729..4385)
                     /locus_tag="PLB46_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75629.1"
                     /translation="MKLFITTLLAITFGLNVIAANPTSTTTKINCTVAPDEAMDPLVE
                     AIVLKDLAKLKSAIEAKSKLEQDIKFIYVRAGVGTISFEKGTPLMYAIQMDWYEGVLE
                     LLRAGAKPNAVRNAEPSGFYHLGCLAEKIEGITPMYLATLHANADILKIMIIADGDTR
                     GSMNVFYSNKCNVNGTNVKMAMWHMRENGPASMVEIIKDAKKAAWAQNGLPAEGQRPL
                     "
     gene            complement(4501..5145)
                     /locus_tag="PLB46_03595"
     CDS             complement(4501..5145)
                     /locus_tag="PLB46_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF75630.1"
                     /translation="MNPIAKILIADDHKLFLDGLNMLLRNQPNFLIEGMAYNGAEVLE
                     SLKSNQYDMVITDLNMPGMGGVELVKSIKEQFPQVKLLVITMNDDREIVTEILLAEAE
                     GYLLKNSGKEQLLTAINNILGGKTHYEPEVINLMLDRVRKTTQTNNFNAQLSTREKEV
                     LDLIMQEFTSKEIAEQLFISKQTVDTHRINIMQKTGAKTLVGLIKMVIQQEGHS"
     gene            complement(5142..5654)
                     /locus_tag="PLB46_03600"
     CDS             complement(5142..5654)
                     /locus_tag="PLB46_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF75631.1"
                     /translation="MESIQQDESEKELITNALKILDDAAIETRNISHNLMPSSLSDLG
                     LVSAIRELSRRINKAGGLQIQFTCDSEFNETDITRSTAIYRILQEVLNNMIKHSGATA
                     IQITLSQTIQHIQLVVTDNGIGFKVSEINNSKGIGWSNIFARAAVIKGELEITSTPQK
                     GTKIMLTAPI"
     gene            complement(5675..6763)
                     /locus_tag="PLB46_03605"
     CDS             complement(5675..6763)
                     /locus_tag="PLB46_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75632.1"
                     /translation="MHTRFIFLWIFFISAFGINVANAQLAVIDSLIKIGDFSEAQKLT
                     LQMLKTASETGDCANEVMANFKNAEIAALLKDDFRAIQFCQDGIAKAKGCDADSAYWL
                     CIRYLGGMYFGHNPDSATYYLKLAYELKKNSNWHRLISSTCGMIANNYTENFKDTAQS
                     LIWYERSMKHALLSPDSAALGYAYLRYAAFLGNAGNCVEAIPMMQESYAIFKRMNDTE
                     GMMFSLKSLAQTYNTCGVKDSVFYVMYRILDLQDTIYKNETARKTAEYRTIYETEKKE
                     KENVIKDAQIQKQQEQRKVIIIAFIVVFVLVILIFVTIYYRNQLKLKTENQRKLAEEQ
                     LLRLKNVVEAEEKERTRIARELHDGVGI"
     assembly_gap    7312..7411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7632..9137)
                     /locus_tag="PLB46_03610"
     CDS             complement(7632..9137)
                     /locus_tag="PLB46_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75633.1"
                     /translation="MSKLLNSEQIKSQWKPLLELITAMTASEKGYFVKSASGFTKQQL
                     PTYMQLFHLIDKQGFESDMQVREKLGTGKTNIHSIRNFLYAQILKSLRAYHSEKNTQF
                     KIRELLDYAELLSEKGLLQQSQHFTDLGIALSDPVTLPAYQIIFQTQQIQLLRSYDEE
                     EKIKKTDEIVLSITESAEIIKHAYITRQGLTKALFYVNTYFPLRNQIVKMEVYQLLHE
                     LISLPDTEKQNYRFRNSRNAAISLLYRLLNDWDNALFYQEKTIRIFEQMQTQLLNRNI
                     PAIGAYYNYISLFINKGDRINYAIQLKKLQALPVSGKAEEHYLKAVVTQLQLDDIIFN
                     KDIFNGKQIVRDTIIFLEEKHPIVSIYHDTLLRLAAYFIYDKNYVDALDVINKLLNSN
                     FQHSLRSFVVHVKLMNILVHFELNNYLLLPGLIRSVYRFMMQQELKYEIEKAILNFFK
                     RTFVVTNPMQLEKEFEKLLHQLEYISNDSYEQQALLSFFDYRFWIAGKLKA"
     gene            9261..11243
                     /locus_tag="PLB46_03615"
     CDS             9261..11243
                     /locus_tag="PLB46_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75634.1"
                     /translation="MKNLTLYCALSLCLSANAFAQNLVWMASSDASSPTRNDQAFTTE
                     LDLSGNHYVAGNYADTLFIDGIAYPNYLNDGSNDAYIAKYDANGNVLWMRTINGPSSQ
                     LIKSMKVNATTGDCYVSGSFIADLYVNGTKLNSQTFSGFSTNFYRSFIMRFDTNGNLI
                     WSDNTFSISGYAIDGGESIALSPLGDYVYMQNGYIGDVVFESGPSYLPSGFGVQGILL
                     TKMSTSTGAIVASRNDIERYLVDGYILETDKVGNVIYAGSHRRTCMFEVDPTAFGTCD
                     TNPAYAIPDGFVWKMNSSFGSIWGKEINGTGFEIVSALGTDAQNNIYIYGTFTSNADF
                     NGTVLTPVAGKSNAFVAKLNSAGNYVYLKQFSADQLYMTEYPTDAEAPFAVDAKGNTF
                     LGGAFSGTLDFNGNTLTSDILPFLFYANGYLIKLNSSGNFRWAEKFEGTTGPFDETAV
                     HGISVLGSYLAVGGEFANYNVYQTDTVFADANAYFISSIQDCDVTIQISASSNLVSVA
                     FPVTLSTPIKPGYSYQWQRNNVDIPGATSNTYVTTLTGNYKVIVTAGVCEIASRRIKL
                     NPHPRFGNIDDATIAVYPNPANENIYIQLPPSQVNTTFSLIISDLSGRTVYSENTTSD
                     ESGLMHVQLQNSISSGTYFIQLIGDNYSATTPIVIE"
     gene            complement(11306..>11639)
                     /locus_tag="PLB46_03620"
     CDS             complement(11306..>11639)
                     /locus_tag="PLB46_03620"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF75635.1"
                     /translation="FFLSLPQPTQSTFLALSNIILNHHPSITPEWKYSMPFFYYNGKM
                     LCYLWTNKKTRQPYIGFADGNKMDFPELIQEKRSRMKIFMIDAEKDLPVKKITLLLKM
                     AIEILEKK"
BASE COUNT         3427 a         2230 c         2124 g         3758 t
ORIGIN      
        1 aaaccctcaa agtcagcaaa aaggcggcca aagcggcatg agtaaagagt tagcagaaat
       61 ggcgcaaaaa caagccatga tacgcgaagc cctcgaaaaa atggcaaaag agctgggcgg
      121 tggcaatact gaagatggca agttggcaaa acagctgcag caactggccg accagatgga
      181 taaaaccgaa gaagatattg ttaacaaaca aatttctata gaaaccctga aacgccagga
      241 agaaatcctt acccgattgc ttgaagccga ggaaagcgag cgcaaacgca agcaggacaa
      301 cgaacgcaag tcaaatacgg ccaacgaaat taaccgtccg gttccgcctg caatagaaga
      361 atacctcaaa aaacgcaatg cagaactcga tttatacaaa actgtttcac cggaattgaa
      421 acctttttac aaaaatttag tagaagaata tttgcggtct attagctact aaactgtaaa
      481 cggggtaaaa tgtcagcaga aaccataagt aaacaaatca gtcttaaatc ccaaccatcc
      541 aatattgtat taatcgaacc ttttatcgaa gaggtgcgaa tgcggctcaa tattgccgac
      601 gaaatgtatg gcaatatatt ggtatgcgtt acggaagcgg ttaacaatgc cattttgcac
      661 ggcaataagg cagatgagag caaagaggta gtggtgagta tccaaaagga gcagaacaaa
      721 cttacctgct tagtaaaaga ccagggttgt ggctttgact ttaacaactt gcccgacccg
      781 acagctccgg aaaatataga aaattgtggt ggtcgtggca tcttcctcat gaagcacctc
      841 agcgacctgg tagttttttc taacgatggc tctaacgtag aaattcaatt ccgcctctga
      901 tgtctgcgat tcaatttgtc aacaaaaaaa caaggtttaa gcttaaagaa caggagcgta
      961 tcgcttcatg ggttgagcgc gtcgttttaa tgtatcacaa atttccaggc gatattcaat
     1021 atattttttg tgacgatact tttttggctg aaatgaatca gcagtattta caacacaata
     1081 cacttaccga tattatcact tttaattata attccgggca ctacatttca ggcgatatat
     1141 ttatcagcat agatagaata aaagaaaatg ccgctactta caaaacaaca atagaagatg
     1201 aactacatcg tgttatgatt cacggcattt tacatctatg tggattaaat gataaaaccg
     1261 ccaaagacaa aaaactcatg caggaaaaag aaaacgaagc cctcgaaatt cttacccgcg
     1321 tttagatgag tggtccgcta tcgtagccac attacttacc ggtttctcgc aaagctgcaa
     1381 agagtggggt tcataatatg tgttaattca agcgcgctaa gctttaaaac cagtttcacc
     1441 cacctcttcg gatgcttcgc attagcacat aagctcatta tcatccgcct cggcgatcac
     1501 attaattggt acattcacac atcggtacat tggtacatca attagttgtt tctcgcaaag
     1561 ctgcaaagag tgggattcat attatgtgtt aattcaagcg cgctaagctt ttaaaccaat
     1621 ttcacccacc tcttcggatg cttcgcatta gcacatcagc acattaacat atcagcacat
     1681 taattggtac atcggtacat tttcacattg gtacatcatc aaattagcac atcaacaaat
     1741 cagcacatca gcacattaaa tggctgttta attggtacat tcacacatcg gtacattggt
     1801 acatcaactc cccagctgcc acccaaaccc aatcatcaac tgcgtatttc cttcccacgg
     1861 atttgtcacc tgattattcc ttgcctgtat atgccaataa ttaaaattaa tatatcgcga
     1921 atgcaattca tccgggttta agtaaatttt tgcattgagt tgattaaatt ttctttcttc
     1981 cgaatcagat acattgtccg gattattttc tccataacca tattgcacca ttacccattt
     2041 atatctggca aatggttgtg caaacaatcc ataataactc atgtggtcca gagcaagtcg
     2101 ccccattaca ttaaaaccaa aatccatatt ttgattgata tcatatttaa attttaatcc
     2161 gcctgcaaat gcagcatttg cataaaatcc agaaccgtat cccacaccga gttgcaggtc
     2221 gataatatct cttatatgaa atcggttttt gggagctgtt ttaaataata cttcaattgt
     2281 acttatcctg gcttctccat ataatgcata atattttccc gaccacaaat cagcagtttg
     2341 aaacgcatcg ttggatggtg aattaatggt tcctaaagat atggcgtaat gactaaagtc
     2401 gtttggcttc gagtatttac cgggagcttg tgccattatc agcaatggaa aaaataataa
     2461 taaatatgtg cttaaatatt tcatgttttt atatttaata aaagtacatt tcaataaatg
     2521 cgctttttgg gaattaaaac atttgtgttg agatagcaga aaaattattt tattgacgtt
     2581 accttggttt caactaagat ggcgccatcg gcatcgtaca ctctcgctaa agtaaacatg
     2641 cccaattcgg gacaatacca gctttcaatt aaattttctg tatagccata ttcgttggtc
     2701 atactaaact tatcaatttt agccgctatc cgttctttag cacgctgata cgattttttg
     2761 gaagctaaat cagaaaaata gtcttttatc tttttgctta acaccgggtc gtcaaaataa
     2821 acagtgggta tttctgtaac tacactgtta tttgttgttt ttatccattg tgcattaaac
     2881 attttataag tcgtatacga ttttcccgaa atcaccatat cttcctttcc aattaccgta
     2941 tagggggtat aaagtgtaat ggtattgata tccattttgg ttgatgttct aacatttaca
     3001 aaaccggaat agttataacc atcgccacca tcaagattaa agtaccgtct cgaagtattt
     3061 aaaaaatcgt aaggaaataa attcatttca gataaatgcc cgggaataat atttccaatt
     3121 tcgatattac gcggaaatgt ttttgcacta taataattca ttccaatagt atctcctttt
     3181 gcatcaacta tcggataaaa ggcggcatta ttaaataagt aacagacagt atctcctttt
     3241 tgttgaattt ttatattatt acctccgctg gcgccaatag ttgcggtgca attgaaagtt
     3301 atcatttctt ccgtttctga tttttcattt acaaccaaag gaagcgaacc tgttgtggcc
     3361 ggtggtatag ttccggccgc aacatccgca ttaaatttat caactgcaat ttcttttttt
     3421 tcatcctttg tttttaccca tttaataggg tcaattaaca atggtgtttt ataagtataa
     3481 acggttgatt ctattatcat accctcctta agtaaaaggg tgttgctgtt ttgagcgtta
     3541 atttttgtaa atccaataat taatatcatc aacatactta gttgcaactt agctactatg
     3601 tattgtctga taaattgaga tttgtttgca ggagttcccg gttcaaaatc tacaagcgtt
     3661 gtgttgtttt gatgttgcat atcacacata aattttcaag atttagttaa aaattgttgt
     3721 gcgtatgctt atagtggtct ttgtccttca gcgggtaagc cattttgcgc ccaagcagct
     3781 ttttttgcat ccttaattat ttctaccatc gatgccggtc cgttttcacg catatgccac
     3841 attgccattt ttacattggt gccgttaaca ttacatttat tactataaaa aacattcata
     3901 gaacctctgg tgtcaccgtc ggcaatgatc ataattttta aaatgtcggc attggcatgt
     3961 aatgttgcca aatacattgg tgttatacct tcaatttttt cagcaaggca tcccaaatga
     4021 taaaatcctg atggttcggc attgcgcacc gcatttggtt ttgcacccgc tctgagtaat
     4081 tctaaaacgc cttcatacca atccatttgg atggcataca ttaacggtgt gcctttttca
     4141 aaagaaatag ttcctacacc ggcgcgaaca taaataaatt taatgtcctg ttccagtttt
     4201 gatttagctt caattgcaga ttttaactta gctaaatctt ttaaaacaat tgcctccacc
     4261 agtggatcca tagcttcatc cggtgcaacg gtacaattaa ttttggttgt ggttgatgtt
     4321 gggttagcag caattacatt taatccgaat gtaatcgcca gcaatgttgt gataaataat
     4381 ttcatagatg tattttatga ctacaaaact attttcaatt gcaagcgcgg tcaatacata
     4441 tttatactga aaatatatcc atacaaatat ggataagctt cttggcgaag cgtttccgct
     4501 tcaggagtga ccctcttgct gaatcaccat tttgataagc cccaccagcg ttttagcccc
     4561 tgttttttgc ataatattaa tacgatgcgt atctacggtt tgtttgctga taaacagttg
     4621 ttccgcaatt tctttactag tgaattcctg cataattaaa tcaagcactt ctttttcgcg
     4681 agtacttaac tgtgcattaa aattatttgt ttgtgttgtt ttgcgaacac ggtctaacat
     4741 taaattaatt acttcgggtt cataatgcgt tttcccgccg agtatattat taattgcagt
     4801 taacaattgt tcttttccgg agttttttaa aagatatcct tccgcctctg ctaataaaat
     4861 ttcggtaact atttctctgt catcattcat ggtaatcacc aataatttaa cttgtggaaa
     4921 ttgttccttt atcgacttca ctaattctac cccacccatg ccaggcatat tgaggtcggt
     4981 aataaccatg tcgtattgat tactttttaa actttcgagc acctctgcac cattataggc
     5041 catgccctca attaaaaaat tgggctgatt gcgcaagagc atatttaatc catccaaaaa
     5101 taatttatgg tcgtcggcta ttaatatttt cgctattgga ttcatattgg tgctgttaac
     5161 ataattttag tgcctttttg gggggtagat gtaatttcca gttcgccttt aatcaccgct
     5221 gctcgtgcaa aaatattact ccaaccaatg cctttcgaat tatttatctc acttacctta
     5281 aacccaatgc cattatctgt tactactagt tggatatgct gaattgtttg cgataaagtt
     5341 atttgaatag ctgttgcacc cgagtgttta atcatgttgt ttaatacttc ctgcaaaatt
     5401 cggtaaatgg ctgtagagcg ggtaatatct gtttcattaa attcggaatc acaagtaaat
     5461 tgtatttgta atccgcctgc tttattaatt cgccttgata attcgcgaat agccgacacc
     5521 aatccaagat cagaaagact tgatggcatt aaattatgtg aaatatttct tgtttctata
     5581 gcggcatcat ctaaaatttt taatgcgttg gtaattaatt ccttttccga ttcatcttgt
     5641 tgtattgatt ccacatgcat ttttgcggcg ctgattaaat gcccacacca tcgtgtaatt
     5701 ctctggcaat acgcgttctt tctttttcct ccgcttccac cacatttttt aatcgcaaca
     5761 attgttcctc tgctaacttg cgttgatttt cggtttttaa ttttaattga ttgcggtaat
     5821 atattgttac aaatattaaa attaccaaaa caaacacaac aataaatgcg attataatta
     5881 cttttcgttg ttcttgttgt ttttgaattt gtgcatcttt tattacattt tctttttcct
     5941 ttttttcagt ctcataaata gttcggtatt ctgcagtttt tcttgccgtt tcatttttat
     6001 aaatggtatc ctgcaaatcg agtatcctat acatgacata aaacaccgaa tcttttacac
     6061 cgcaggtatt gtatgtttgc gccagcgatt ttaaagaaaa catcatgcct tcggtatcgt
     6121 tcatacgttt aaatatggca taactttcct gcatcatcgg aatggcttca acacaatttc
     6181 ctgcattacc caaaaatgcg gcgtatcgca aataagcata tccaagcgca gcggaatcgg
     6241 gcgagagtaa tgcgtgtttc atggaacgtt cataccaaat taaggattga gcagtgtcct
     6301 taaaattttc tgtataatta ttggcaatca tgccgcatgt gctcgaaata agtcgatgcc
     6361 agttgctatt ttttttgagt tcgtaagcaa gttttaaata ataggtcgct gagtcggggt
     6421 tatgtccaaa atacattcct cctaaatacc taatacacaa ccaataagca gaatcggcat
     6481 cacaaccctt tgccttggca ataccatcct gacaaaactg aattgcacga aaatcgtctt
     6541 taagcagtgc cgctatttct gcatttttga aattggccat cacctcattc gcgcaatcgc
     6601 cggtttcgga agcggttttg agcatttgca gcgttagttt ctgagcctca ctaaaatctc
     6661 cgatttttat taaactgtcg ataacagcta attgcgcatt ggcaacattt atcccaaatg
     6721 ctgaaatgaa aaatatccat aaaaaaatga atctagtgtg cattaaaatg gaaggtataa
     6781 tagtagtccg aaatttacaa atacttgatg aggttggttg aaaaaagatg gatgtggtgc
     6841 gtgtgatttg accaatatgc cgccctgtcg ggcttgttgg tgtggggtgg ccgcctggcg
     6901 ggcgggtaac cggtgtgccg ccctatcggg cttgatgtgg tgagttgacc tattagcgga
     6961 gtgttaccag tatgccgccc tgacgggctt gatgtggtcg ggttatcgaa cggtgggtcg
     7021 ggttaccagt atgtcgccct gacgggctca acatatcagg gtgactgagc agtgggtcgg
     7081 gttagcggtg tgccgccttg gcgggctcga tggtgtgggg tggccacctg gccggcgggt
     7141 taccgatgta tcgccctggc gggctgggtt aaagtatcat gaaggttgtt tcttaataca
     7201 ttggcacttc actcagcccg ccagggcggc ataccgttgt atcgccctga cgggctgggt
     7261 taaagtatca cctagattgt ttcttaatac attggcactt cactcagccc gnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggttaaag tatcacctag attgtttctt
     7441 aatacattgg cacttcactc agcccggcag ggcagtatac cgttgtatcg ccctgacggg
     7501 ctgggttata gtatcatgaa gagtgtttct taatataccg gcacttcact cagcccggaa
     7561 gggcggcaga ccggtaacac aacaatagca aatactatat agcccgtcag ggcggcatac
     7621 tggtttctgg atcaggcctt cagtttaccc gcaatccaaa atcgataatc gaaaaacgat
     7681 aacaaggctt gttgttcata ggagtcgttc gagatatatt cgagctgatg caacaatttt
     7741 tcaaattctt tttccagttg catcggattg gttaccacga aagtgcgttt gaaaaaattg
     7801 agaatggcct tttcaatttc atattttaat tcctgctgca tcataaatcg ataaacactt
     7861 cttatcaatc cgggaagtaa taaataatta tttaactcaa agtgaacaag aatgttcatc
     7921 aattttacgt gcacaacaaa cgaccgcaac gagtgttgga aatttgaatt taggagttta
     7981 ttaattacgt ctaaagcatc aacataattt ttatcataaa taaaataagc ggctaaacgt
     8041 aataatgtat catggtaaat gctaacgatg ggatgttttt cttccaaaaa aataatggta
     8101 tcccgaacta tctgtttacc attaaaaata tctttattaa aaataatatc atctaattga
     8161 agttgagtca caactgcttt tagataatgc tcctcagcct ttcctgaaac cggtaatgct
     8221 tgcaattttt ttaattgaat ggcgtaatta attctatcgc ctttattgat aaacaatgaa
     8281 atgtaattat aataagcgcc aattgcagga atatttctat tgagtaattg cgtttgcatt
     8341 tgttcaaata tccgaattgt tttttcctgg taaaacaagg cattatccca atcatttaat
     8401 aatctgtata ataatgaaat agctgcgtta cgtgagtttc taaaacgata attttgtttt
     8461 tctgtatccg gcaacgaaat gagttcgtgt aataattgat acacttccat tttaacaatt
     8521 tgattgcgca atggaaaata agtatttaca taaaacaacg ctttggttaa gccctgtcgt
     8581 gtaatatatg cgtgtttaat tatttctgca ctttcggtta tggataaaac tatttcatct
     8641 gtttttttaa ttttttcttc ttcatcataa gaacgcaata attgtatttg ttgtgtttga
     8701 aaaataatct gataagcagg aagtgtaacc gggtcgctta atgcaatgcc taagtcggtg
     8761 aagtgctgac tttgttgcaa caatcctttt tcggaaagta attctgcgta atcgagtaat
     8821 tcacgaattt taaattgggt gtttttttcg ctgtgataag cacgtaaact tttaagtatt
     8881 tgggcatata aaaaattgcg gatgctatga atatttgttt ttccggttcc tagcttttcc
     8941 ctcacctgca tatcactttc aaatccttgt ttgtcaatta aatgaaataa ttgcatataa
     9001 gttggcaatt gctgtttggt aaatccgctg gcagatttta caaaatatcc cttttcactg
     9061 gctgtcatcg ccgtaattag ctccaataat ggtttccact gactttttat ctgttcggaa
     9121 tttagcaatt ttgacataaa tgaaaactaa aataaggtat ttatggctac ttgtccaact
     9181 tcgaaatatc aaaatttaaa attttttgta ttgacttcgc ccgttttcgc taatagtttt
     9241 gccctaaaag aatactattt atgaaaaatt taacccttta ttgtgcgctt tcgctgtgtt
     9301 tgtcggcgaa tgcgtttgcc caaaatttag tgtggatggc ctcttccgat gcttcatcac
     9361 ctacccgtaa cgaccaggct tttaccactg agctggacct gagtggcaat cattatgttg
     9421 caggaaacta cgcagacaca ctttttattg acggtattgc ttatcccaac taccttaacg
     9481 atggttcaaa tgatgcttac attgcaaagt atgatgcaaa tggcaacgtg ctttggatgc
     9541 gcaccatcaa tgggccatct tcacaattga ttaaatcgat gaaggtgaat gccacaacag
     9601 gcgactgtta tgttagcggt tcgtttatcg cagacctgta tgttaatggc actaaactaa
     9661 attctcaaac cttttccgga ttctccacca atttttatcg ttccttcatt atgcgttttg
     9721 atacaaacgg taacctgatt tggtcagaca atactttcag cataagcggt tatgccattg
     9781 acggtggcga gtctatcgca ctttctccgc ttggcgatta tgtatatatg caaaatggtt
     9841 atatcggcga cgtggtcttt gaaagcggtc cttcctattt gccatccgga tttggtgtgc
     9901 agggtatttt gttgaccaaa atgtcgacct caacaggagc tattgttgct tcaagaaacg
     9961 acattgaacg atatcttgta gatggttata ttttagagac cgataaggtt ggaaatgtaa
    10021 tttatgccgg cagtcaccgc agaacatgca tgtttgaagt tgatccaaca gcttttggta
    10081 cttgtgacac caacccggcc tacgctatcc cagatggatt cgtttggaaa atgaacagct
    10141 catttggcag catctggggc aaagaaatta acggaaccgg atttgagatt gtttctgcac
    10201 ttggaactga tgcgcaaaac aatatttata tctatggcac atttaccagc aatgccgatt
    10261 ttaacggaac cgttttaacg cccgttgcag gtaaatcaaa tgcatttgtt gcaaaattaa
    10321 attcagcagg aaattatgtt tacttgaaac aattttctgc agatcagtta tacatgaccg
    10381 aatatccaac cgatgcagaa gcgccttttg ctgttgatgc aaaaggtaat acatttcttg
    10441 gcggcgcgtt ttcaggaaca ttagatttta atggtaacac tttaacgtca gatatattac
    10501 catttttatt ctacgcgaac ggttatttaa ttaaattaaa cagttccggt aattttagat
    10561 gggctgaaaa atttgaaggc actacaggtc cttttgatga aactgctgtg catggaattt
    10621 cagtattagg ttcttaccta gcagtaggtg gagaatttgc aaattataat gtatatcaaa
    10681 cagacacagt atttgcagat gccaatgctt attttataag ttcgattcag gattgtgatg
    10741 ttaccattca aatttcagca tcgtcaaatc ttgtaagtgt tgcatttcct gtaacacttt
    10801 ctacaccaat aaaaccaggg tatagttatc agtggcaacg taataatgtg gacataccgg
    10861 gagcaacttc aaacacttat gtaacaacac ttaccggtaa ttataaagta attgtcactg
    10921 caggtgtttg tgaaattgca tccagaagaa taaaattaaa tccgcatcca cgttttggta
    10981 atattgatga tgcaacaatt gcagtatatc caaatccggc aaacgaaaat atttatattc
    11041 aattaccgcc atcacaagtg aatacaacat ttagtttaat catttcagat ttatccggaa
    11101 gaaccgtgta ttcagaaaat accacctccg atgaatcggg attaatgcat gttcaattac
    11161 aaaacagcat cagcagtgga acatatttta tccaattaat tggagataat tatagtgcca
    11221 ctacaccaat tgtgatagaa taaataaaat cataataaac caaaaaggtc accgcatttg
    11281 gtgacctttt tttatgcaaa attttttatt ttttttccag tatttcaatt gccattttca
    11341 acaacaaagt aattttttta accggcaaat ctttttccgc atcaatcata aatattttca
    11401 tgcgcgaacg tttctcctga attaattccg gaaaatccat tttatttcca tctgcaaatc
    11461 ctatataggg ttgtcttgtt tttttattcg tccacaagta acacaacatt tttccattgt
    11521 aataaaaaaa cggcatgctg tatttccatt cgggggtgat ggatgggtgg tgattgagaa
    11581 ttatattgct cagggctaga aaagtagatt gtgtgggttg tgggagggag aggaaaaag
//
LOCUS       DAORKW010000050        11588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_111_length_11588_cov_5.183560, whole genome shotgun sequence.
ACCESSION   DAORKW010000050 DAORKW010000000
VERSION     DAORKW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111_length_11588_cov_5.183560"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(643..2019)
                     /locus_tag="PLB46_03625"
     CDS             complement(643..2019)
                     /locus_tag="PLB46_03625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF75636.1"
                     /translation="MQTTLTIQQRKKTIFPALSENKLLRYFNFVALYFAQGIPEGMLA
                     LGIPAWMAMNGKTPGEISAFAIIIVLPWSFKFIAAPLMDRYTYLPMGRKRPWVLVGQL
                     GLVLSCINMAFVPDPLNNLNQLMIAAFFISCFGAFQDIATDGMAVDLIPGDQQAKANG
                     LMWGSKVIGISVSLAACSLILNMYDFKTAMLTVAAAIGIIMLVPAFLRERKGEKLAPW
                     SKGKASTETKNLQLNNWQSLFKSLYKVFTLRNSLLIALMLFICQGATKYMGTILPIFT
                     VKELGWTNVEYSNYYASAKLIGGILGMIIGGILIDKYGKKQMINIYFFSFILFISFFS
                     FSTMYWADKNFVFAFMIVQNTIFTLNSIAVFAVAMQCCWKKISASQFTLYMTIANMGQ
                     MTFASFIGPITNNFNWQISLFVFAGFIALAWIVLQFVNIDKQIQQVIHLDKSDLENEE
                     KVAKVAYI"
     gene            complement(2110..2853)
                     /locus_tag="PLB46_03630"
     CDS             complement(2110..2853)
                     /locus_tag="PLB46_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75637.1"
                     /translation="MKIKFNPKPLALYLLIMVMPLWSMAQTKNVISTHRVFPKIDKVL
                     EFERALANHAQKYHSGDAAWRVFAIQTGPDMGGYHITEGPKTWESEDSRGDLGAAHME
                     DWHKNVAIYLTDKQSAGYYEFIDTLSSVAIGDVTDKINITNVYPKPGQGDNVVLMINK
                     LQKTWEASGVSVAVYKSNSSGQFHYLIVTRYKEGLKEKTPGFRKPFKETYEAVNGQGS
                     YSQYQKDLAEYIDKSWSELLFLKKDMSSK"
     gene            3102..7232
                     /locus_tag="PLB46_03635"
     CDS             3102..7232
                     /locus_tag="PLB46_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF75638.1"
                     /translation="MKVNLLFTLLLCSFLKPVHAQLKYDWLTTSQGLSQGYIHDIIQD
                     RDGFMWFGTKDGLNRYDGYTFKEYTYNIYDPNSISNNTVHHLYEDSKGRIWAITDNGI
                     NIYDKNRDNFRRIMHDPKNENSLADDKISLPIIELNDGRFLVMPEEESIHLITLPDDF
                     LASNLAPEIVRLKTPEVHNATHLFKDKHGKVWLLADNILYEFIPDNSSLIKRKEKVDF
                     GQTYTEATGEVWTNDMFFSKIEDTEFYPLFSKNIAKGHGTFFLLEADKHRLWLGITDS
                     AKLEIYDTKNWTKGKPLDPEKCRLYSFSNITPTKFYKDRAGIVWVGTNGYGVRKYTTD
                     TEQFNHIGSGFSVRKIVELPNNQVYAFGWGEIKKFDANLKLITTEKDKLLFKNYEFIV
                     GNDQTIWGFRKKRSSQNEVLVDAIENINPVTGAVKSYKINVDIAKLIAEPKMEDSKGY
                     IWFVGFGGNMYVLNPETAEGKIVSINTDLINPMLKGAQITALYEDAEGTIWAGTETGL
                     AKVNFEFNAPAKTQVRWYNTNPDDIKALSYSHVSSMMDDAADKNILWVATKGGGLNRL
                     DKRSNTFLHITTKEGLCNNVVYGILSDAEGNIWGSTNNGIFCLLMNVNQVSNGWQFRH
                     FTKAAGLQEDEFNTNAYTKLKNGYLAFGGVNGLNIFDPAVVLQKGFIPNVFITKILLG
                     NKEIFPDDESGVMQQSIEFVQGITLNYKQDIVTLEFSSLDFTAPGQNKYRYQLEGIDD
                     GWVESGNRRTATYVHLPSGNYTFKVQGSNSKGEWSSKIVELDVVILPPWWQTWWAYTL
                     YILIIAIGIGSYFKFRLNKAKLQAQLNFEQQEAIRIKELDAVKTQLYTNITHEFRTPL
                     TVILGMANQVKNEPDKFLDKGVDMILRNGENLLKLVNEMLDLSKLESGKMILNLRNGD
                     FIAYLRYIVESFQSLASAQQKQFHFLANADELQVVFDAEKTRQIITNLFSNALKFTPE
                     HGNIYVSVSYQPISSSNMVEVEIKVKDTGIGIPENQIPHIFDRFTQLDNSHTRKAEGT
                     GIGLALTKELIKLMNGTIGVKSPPVGANKGTEFIIRIPMTKSDLIDASQTIIPAHDEK
                     NVKAHTDEKIQINHLNETISNSNVPLILLVEDNEDVVGYTAGCLTDYRLLVGRDGREG
                     FDIAIEKIPDIIITDVMMPYVDGFEMVKKLRQDERTSHIPVIILTAKADIQSKLEGLE
                     SGADVYLQKPFHKEELLLRIKKLLEMRKNLQQYYRKQIGISSPNETHKNEKPETIIEE
                     KAEHEFVIKVREIIEANFNNYTFSVEQLCKLLFMSHSQLHRKLYALIDCSPNKFIRIV
                     RLNKSKELLTNHSLSIATVALECGYNDPGYFTRVFKQEFGITPQEWRQSSSEFQSK"
     assembly_gap    7919..8018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8264..8536)
                     /locus_tag="PLB46_03640"
     CDS             complement(8264..8536)
                     /locus_tag="PLB46_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75639.1"
                     /translation="MRKIKILIIVIITGVLASCSEKKADETEALPETTTTTETKAVEV
                     ETKTPEKTTEITIGEKGAKVETKNGDNKTSVEIKDGTTEIEIKKKD"
     gene            complement(9266..9832)
                     /locus_tag="PLB46_03645"
     CDS             complement(9266..9832)
                     /locus_tag="PLB46_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75640.1"
                     /translation="MELNIIHLKHRHDRFELLKNQLNEQNIKNYRLWEGIVDQNPKRG
                     IAKAHKQIIEWANKQNLPSVLIAEDDIKFTARGAFEYFLQNEPQQYDIYLGGIIYGNI
                     NADNSVNDFAGAHLYKINKRFYNTFLSLPEEIDIDRTLANKGKFIVCKPFAAIQQNGF
                     SDNQKKYRNYNRYLQNRELYKSISHDEH"
     gene            9930..10439
                     /locus_tag="PLB46_03650"
     CDS             9930..10439
                     /locus_tag="PLB46_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75641.1"
                     /translation="MRPQDVAILLKIVLMDSQNWQLVGLANLLKISISEISESLNRSR
                     LAGLIDYNKKKINRQNLLEFLEHGVRYVFPQQPGSMVRGVPTAHSHPDMKKYFISEMN
                     YVWPDNKGDAMGLMIEPFYKKQVEAVKEDQEFYKLLALVDVIRVGKVREIEYAVDELK
                     KNILHGPSY"
     gene            10423..11103
                     /locus_tag="PLB46_03655"
     CDS             10423..11103
                     /locus_tag="PLB46_03655"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRF75642.1"
                     /translation="MGHHINKVRIKAVATALDTLKEKVVFVGGATISLYPDQPVFEVR
                     PTDDVDVIIEILNYSDRTELEEKLRSIGFAHDIESGVICRYRIQGIVVDIMPTNDSSI
                     GFTNIWYPEGYKNAVNYKIDERCIVKILSAPYFIATKLEAYKNRGKDDGRTSKDFEDI
                     IYVLENREVIWDEINNSTENLKNYLLTEFRNLLKNPNLFEWIDCHVERGSPPASYFIM
                     EEIEKFTA"
BASE COUNT         3768 a         1961 c         2061 g         3698 t
ORIGIN      
        1 gggtaattat gtcaaatagc aaaagggccg aagcatcagc aacaagtgaa tagataccta
       61 acttgaaccc aataaaccta aaatattgat ctagtttaaa ataatattat ttatagccat
      121 ttttatttca ttaaaattac ataaaaaaca gaatatcaat agattacgat tttgttttag
      181 ccaaatttat agattttacc ctgtctcata accccatagt atgtcctctt tgggagctgg
      241 ctcccaaagc aatcgggaac ggttgtatgc tgtgctgatg tgattgcgct agcgaggtgt
      301 aaaccaattt cgctattgac attctatgcg aaaatgagca atgcttgtgc ttagattgca
      361 acagaagaac ggaaaataat tttcgaagca aactcacaac cggtctgaag accggggtgg
      421 cgtctggagt cggagactcc acgacaactc aaagcccggg tattttcatg caaagctcat
      481 attgttgcta tgttactatg caatcggcaa caaacgccat tggattttag caatacattt
      541 attgcatttg gtttaatgca ataaatgttt aacttttata tagagcaatt gctccatgta
      601 taactaataa aaaacaacga agagttgaac ccggtgtgtg gttcaaatat atgcaacctt
      661 ggcaactttc tcttcgtttt ccaagtcgct tttgtcaagg tgtatcacct gttgtatttg
      721 tttatcaata tttacaaatt gtaatacaat ccaagccaat gcaataaaac ctgcaaatac
      781 aaaaagtgaa atttgccagt taaaattgtt tgttatcgga ccgataaacg atgcaaatgt
      841 catttgcccc atattggcga tagtcatata taatgtaaac tggctcgctg aaatcttttt
      901 ccagcagcat tgcatggcaa cagcaaaaac ggctatacta tttaaagtaa aaatggtatt
      961 ttgaacaatc ataaaagcaa atacaaaatt tttatctgcc caatacattg tagaaaagga
     1021 aaaaaaggaa atgaataata taaagctaaa aaagtaaata ttaatcattt gttttttgcc
     1081 gtatttatct atgaggatcc cgccgataat cattcccaaa attccaccga tgagttttgc
     1141 agaagcataa taattagaat attcaacatt tgtccaccct aattctttca cggtaaaaat
     1201 aggaagtata gtgcccatat attttgtggc accttgacaa ataaataaca ttaatgcaat
     1261 gagtaaagaa ttacgcaatg taaatacttt atataatgat ttgaaaaggg attgccagtt
     1321 attcagttgc aaatttttcg tttctgtgga agcctttccc tttgaccatg gagctaattt
     1381 ttcacccttt ctttctctta aaaatgcggg aactaacatg attattccga tagcagctgc
     1441 tactgtaagc attgcagttt taaagtcata catatttaaa attaaactac atgctgcaag
     1501 tgaaacagat atgccaatta cttttgaacc ccacattaaa ccattggctt ttgcctgttg
     1561 atctccgggt attaagtcaa ctgccatgcc atcagttgca atatcctgaa aagcaccaaa
     1621 gcaagaaata aaaaatgcgg ctatcattaa ttgatttaag ttgttaagag gatcaggaac
     1681 aaaagccata ttaatacaac tgagcaccaa acccagttgt ccaacaagta cccatggtcg
     1741 ttttcttccc atcggcaaat aggtatacct atccatcaac ggagccgcaa taaatttaaa
     1801 actccacggc aaaactataa tgatagcaaa tgcagaaatt tcgcccggtg ttttcccgtt
     1861 catagccatc catgcgggta ttcccaaggc aagcatccct tcaggaatac cttgtgcaaa
     1921 atataaggct acaaaattga aatatcgcag taatttattt tccgagagtg ccggaaaaat
     1981 ggtttttttc ctttgctgaa ttgtcaaggt ggtttgcata atatgcgcgt tgaaaatttg
     2041 aaagtgggaa gagtaataaa ttattactct tcccttttga agtgttgtta attaataaag
     2101 gtttctagat tattttgagc tcatatcctt tttcaaaaaa agtaattcgc tccagctttt
     2161 atcaatatat tccgcaaggt ctttctgata ttgcgaatat gatccttgtc cgtttacagc
     2221 ttcatacgtc tctttaaatg gtttgcggaa accgggagtt ttttccttta aaccttcttt
     2281 ataccttgta acaattaaat aatgaaattg tcctgaagag tttgatttat acacggcaac
     2341 cgaaaccccg gatgcttccc aagttttttg taatttatta atcatgagca caacattatc
     2401 gccttgaccc ggtttcggat acacattggt aatatttatt ttatccgtaa catcccctat
     2461 ggcaactgaa cttagtgtat caataaattc ataatagcct gccgattgtt tgtcagttaa
     2521 ataaattgcg acgtttttat gccaatcttc catatgggca gcaccaaggt cacctctgga
     2581 atcttcactt tcccatgttt ttggtccttc agtaatatgg tatccaccca tatcagggcc
     2641 tgtctgaatt gcaaaaaccc tccaggctgc atcacctgaa tgatattttt gagcatggtt
     2701 ggcaagtgcg cgctcaaatt cgagcacttt atcaattttt ggaaataccc ggtgtgtaga
     2761 tattacattt ttggtttgtg ccatactcca aagtggcatt accataataa gcagatacaa
     2821 agcaagtggc tttgggttaa atttgatttt cattgtcttt tgattttgtg gttaatgttt
     2881 aattagcaat cataaattaa tcctccaaaa ttccaccata caggcatttt tcatgcgcat
     2941 ggattaacca aaaaaccgaa tttttcgtgc aaagaatggg acaaatggcc caacaatagg
     3001 acaaaatcac catattttaa accctttggc aattttgtat tcaggaagtg taaataattg
     3061 cgttttgaat ttacctttat tgacaaaagt tgctgcctta tatgaaagtc aaccttttat
     3121 tcaccctttt gctctgtagt tttttaaagc ccgttcatgc gcagctaaaa tatgattggc
     3181 ttaccacctc ccagggctta tcgcaagggt acattcatga tattattcag gatagggatg
     3241 gttttatgtg gttcggaact aaggatggtc tcaaccgcta cgacggatac acttttaaag
     3301 aatacaccta taatatatat gaccccaatt caataagcaa caacaccgtg catcatttgt
     3361 atgaagatag caaggggaga atttgggcta ttacggataa tgggataaat atttatgata
     3421 aaaacaggga taattttcga cgcatcatgc acgaccccaa aaatgaaaac agccttgcag
     3481 atgacaaaat atcattacct attatcgaat taaatgacgg gcgtttttta gtaatgcctg
     3541 aagaagaaag tattcatctt ataaccttac ctgacgattt ccttgccagc aaccttgccc
     3601 cggaaattgt tcgtttgaaa actcctgaag tgcacaatgc cactcattta tttaaagata
     3661 aacatggaaa agtatggctt ttggcagata atatcttgta tgaatttatt ccggataaca
     3721 gttcacttat caaacgaaaa gaaaaagtgg attttggtca aacgtatact gaagctactg
     3781 gtgaagtttg gactaacgat atgtttttca gtaaaattga agacacggaa ttttatcctt
     3841 tattttcaaa aaatattgct aaaggacatg gtacattttt cttgcttgaa gcagataaac
     3901 acagattgtg gctgggcatt accgattcag ccaaattaga aatatatgat actaaaaact
     3961 ggacgaaagg gaaaccgctt gatccggaaa aatgccgcct ttatagtttt tcgaatataa
     4021 cacctacaaa attttataag gaccgcgctg gtattgtgtg ggtaggaacc aatggttatg
     4081 gcgtgagaaa atatacaacc gataccgaac aatttaatca tatcgggtcc ggattcagtg
     4141 ttcgcaaaat agtggaattg ccaaataatc aggtatatgc tttcggttgg ggagaaatta
     4201 aaaagtttga tgccaaccta aaattaatca caacagaaaa agacaaatta ctttttaaaa
     4261 attatgaatt tattgttgga aatgaccaaa cgatttgggg tttccgcaaa aaaagaagta
     4321 gccaaaatga agttcttgta gatgcaattg aaaacatcaa cccggtaaca ggagcagtaa
     4381 aatcttacaa gattaatgta gacatagcga aattaatcgc ggaaccaaaa atggaagata
     4441 gtaaagggta tatttggttt gttggttttg gcggaaatat gtatgtatta aatcctgaaa
     4501 ctgcagaggg taaaattgtt tccataaata cagaccttat taatcctatg ctaaaaggtg
     4561 cacaaataac ggcattatac gaagatgccg agggcactat ttgggcagga actgaaacgg
     4621 gcttggcaaa agttaacttt gaatttaacg cgcctgctaa aactcaggta agatggtata
     4681 atactaaccc tgatgatatt aaagccttaa gttatagcca tgtttcaagt atgatggatg
     4741 acgctgccga taaaaatatt ttatgggttg ctacaaaagg tggtggatta aacaggttag
     4801 ataaacgttc aaatacattt ttacatatta ctactaaaga aggtttatgt aataatgtgg
     4861 tgtatggtat tttatcggat gcagaaggta atatttgggg tagtaccaac aatggtattt
     4921 tttgtttatt gatgaatgta aatcaagtaa gtaatggctg gcaattcaga cattttacca
     4981 aagctgcggg attacaggaa gatgaattta ataccaacgc ttacaccaaa ctgaaaaatg
     5041 gatatttagc ttttggcggt gtaaatggat taaatatttt tgatcctgcc gtggtattgc
     5101 agaaaggatt tataccaaac gtttttatta caaaaatact gttaggtaac aaggaaattt
     5161 ttccggacga cgaaagcggc gtcatgcaac aaagtataga atttgtgcaa ggcattacac
     5221 tcaattacaa acaggatatt gtaacacttg aattttcttc gcttgatttt actgccccgg
     5281 gacaaaataa ataccggtat caactagaag gtattgatga tggctgggta gaaagtggta
     5341 accggcgaac agcaacctat gtgcatctgc catcaggaaa ttacacattt aaggtgcagg
     5401 gaagtaacag taagggtgag tggagtagta aaattgtaga attagatgtt gtaattcttc
     5461 caccctggtg gcaaacctgg tgggcttata cgctatatat tttaataatt gcaataggca
     5521 ttggaagtta ttttaaattc agattaaata aagcaaaatt acaagcacaa ttaaattttg
     5581 aacagcagga agctataaga attaaagaat tagatgccgt aaaaacacaa ctctacacaa
     5641 atattacaca tgagtttcga acaccgctca ccgttattct ggggatggca aaccaggtta
     5701 aaaatgagcc tgacaaattt ttagataaag gtgtagacat gattttgcga aatggagaaa
     5761 atttattgaa gttagtaaat gaaatgctgg atctttcgaa attggaaagc ggtaaaatga
     5821 tactgaattt gcgcaatggc gatttcattg cttatttaag atatatagta gagtcttttc
     5881 agtcgctggc atcagctcag caaaaacaat ttcatttttt ggcaaatgca gatgaattgc
     5941 aggtagtttt tgatgcagaa aaaacaaggc agattattac aaatttattt tcaaatgcat
     6001 taaaatttac acctgaacat ggcaatattt atgtgagtgt ttcgtatcaa cctattagca
     6061 gcagtaatat ggttgaagta gaaataaaag tgaaagacac aggcattggt attcctgaaa
     6121 atcaaattcc acatattttc gaccgcttta cccaattaga taacagtcat acacgaaaag
     6181 cagaaggtac aggcattggt cttgccctta ccaaggaatt aataaaatta atgaatggta
     6241 ccataggtgt aaaaagtccg ccggttggtg caaataaagg aacagaattt attatccgta
     6301 tccccatgac aaaatcagat ttaattgatg cctcacaaac tataatacct gcccatgatg
     6361 aaaaaaatgt aaaggcacat actgatgaaa aaattcaaat aaatcaccta aatgaaacta
     6421 taagcaacag taatgtccct ttaattttgc tggtggaaga taatgaggat gtggtgggct
     6481 atacagcagg atgtttgacc gattaccggt tgttggttgg aagagatggg agagaaggtt
     6541 ttgacatcgc aatagaaaag atacctgata ttattattac cgatgtgatg atgccttatg
     6601 tagatggttt tgaaatggtg aaaaaactga gacaagatga acgcactagc catatccctg
     6661 ttattatatt aactgctaaa gcagatattc aaagtaagtt agaagggttg gaaagtggag
     6721 ccgatgtgta tttgcaaaaa ccatttcata aagaagagct gttattgcgt atcaaaaaat
     6781 tattagagat gcgcaaaaac cttcaacaat attaccgcaa acagataggt atttcatcac
     6841 caaatgaaac tcacaaaaat gaaaaaccgg aaaccattat tgaagaaaaa gcggaacatg
     6901 aatttgtaat caaggtaaga gaaataatag aagccaattt taataattat accttcagtg
     6961 ttgagcaatt gtgcaagtta ttatttatga gccactccca attgcatcgt aaattatatg
     7021 cactcatcga ttgttcaccc aataaattta tccgtattgt acgattgaat aaatcaaaag
     7081 aattactgac taatcattcc ttgagtattg ccacagtagc tttagaatgc gggtataacg
     7141 accccggata ctttacccgc gtatttaaac aggaatttgg aataaccccg caagagtggc
     7201 gtcaaagcag cagtgaattt caatcgaagt gaatttttgc gatgggtaat tgatgtacca
     7261 atgtgcgaat gtaccaatgt accaatgcgc aaagcgtccg ccgaggcgga aacagccaat
     7321 tttgagtaag catttcatac aaagctccgt agggggctga cctatgtgtc agctcaatgt
     7381 gctgcaaacc agataattta caaatgaggc aactgcggta actgctattc gaacagcgat
     7441 tttgagcagg attgtgatgc ccccattagc taatttgcta atcacctaat tagctaattt
     7501 cttcccgcct tggatgctaa ttaaccctcc cgattctaat cagaattgtc atttaatcga
     7561 taaactccat tagctaattt gctaatcacc taattagcta atttcttccc gccttcgatg
     7621 ctaattaacc ctcctgattt taatcagaat tgccctttaa tcgataaact ccattagcta
     7681 atttgctaat cacctaatta gctaattgct tcccgccttc gatgctaatt aaccgtcccg
     7741 attttaatca gaattgcccg ttaatcgata aactccatta gctaaattgc taatcaccta
     7801 attagctaat tgctacccgc cttcgatgca aattaaccgt cccgatttta atcagaattg
     7861 ccctttaatc gataaactcc attagctaat ttgctaatca cctaattagc taatttctnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa tttgctaatc acctaattag
     8041 ctaatttctt cccgccttcg atgctaatta accgtcccga ttttaatcag aattgtcatt
     8101 taaccaatca acccccatta gctaatttgc taatcagcta atttgctaat tgcttcccgc
     8161 cttcgatgct aattaaccgt cccggtttta atcagagttg tcatttaatc gataaacccc
     8221 atttgctaat cagctaattt gctaattagc taattctcca tcattaatct ttcttcttaa
     8281 tctcaatctc cgtagtcccg tctttaattt cgacgctggt tttgttgtca ccattttttg
     8341 tttccacttt cgcacctttt tcaccaatag tgatttctgt agttttttca ggtgtttttg
     8401 tttctacctc aactgcttta gtttctgtag ttgttgtggt ttcaggtagt gcctcggttt
     8461 catctgcttt cttttcagaa caagatgcta aaacaccggt aataattact ataatcaata
     8521 ttttgatttt tctcatttac caaagttaat ctatttaagt tagtaattgt gttttttttg
     8581 ttttttggcg gttgaattgt tttccggctt agcgaaggtg gtgattttac ccctgaacta
     8641 acatacgaag cacgcacttt aaatttacga aaaaaactgc catggtaaga acgaactcac
     8701 tgcttttgac aagccgctgt gagtggtaga tttatttttt agcttcaaat gactggatac
     8761 attatttaca tgctttaaaa gaaaacaata ataataaatg catattttat ggattaattc
     8821 acttgatatt tcagattcat atttggtcat tttaactgtg acacggattc tgtataaccc
     8881 tccctcattt gtttcttttt cttcggagat ttcatcagaa attgttactt caatttcgtt
     8941 gccttcaggt catttagcat ttcgtttaaa attgtataat actcctcagg cagtaaatgg
     9001 cccattgtaa aatattctgt tttatatcgg attgttatgt tgtggaaatg cgtaattcaa
     9061 ttactttttt aaaaatattt tttctacttc ggaaatatta cattgcataa accatttttc
     9121 tcagccattt attttaccaa atacatgacc tataacccat gtcgtacttg ttgcggaatt
     9181 taaatatgcg ctaaggatgt aatcaaaata tggataattt gggttgagtt gctttaaaaa
     9241 atatcgcgcc tcttcaagtt ttctttcagt gttcgtcatg agaaattgac ttgtataatt
     9301 ctctattttg taaataccga ttataattcc gatatttttt ttgattatca ctgaacccat
     9361 tttgttgaat agctgcaaat ggtttgcaaa ctataaattt tcctttattg gctaatgttc
     9421 tatcaatatc tatttcttcc ggcaatgaca gaaaagtatt ataaaatctt ttattaattt
     9481 tatatagatg agctcccgca aaatcgttta ccgagttatc agcattaata tttccataaa
     9541 ttattccacc aagatatatg tcatattgtt ggggctcatt ttgaagaaaa tattcaaaag
     9601 ctcctctagc cgtaaatttt atgtcgtcct cagcaataag tactgaagga aggttttgtt
     9661 tgttggccca ttctataatt tgtttatgag cttttgcaat gcctcgtttg ggattttggt
     9721 ctacaatgcc ttcccataat cggtaatttt ttatattttg ttcatttagt tgatttttaa
     9781 gtagttcaaa tctatcgtgc ctgtgtttta aatgaatgat atttagttcc attacaacga
     9841 atataagaat aatttatact atttctagtt tcgcgatttg cgaaatgcga aatttgttgt
     9901 atatttgtcc tatgagaaaa cataatggca tgcgcccaca agatgtggct atactattga
     9961 agattgtctt gatggattcc caaaattggc aattggtagg acttgcgaat ttgctaaaaa
    10021 ttagcatttc agaaatttcc gagtctttaa atagaagtcg cttggctgga cttattgatt
    10081 ataacaaaaa gaagattaat cgacagaatt tattggaatt tttggaacat ggtgttcgat
    10141 atgtttttcc tcaacaacca gggtcaatgg taaggggggt gccaacagct cattcacatc
    10201 ccgatatgaa aaagtacttt ataagtgaaa tgaattatgt ttggccagat aataaaggag
    10261 acgccatggg attaatgatt gaaccgtttt ataaaaaaca ggtagaagca gttaaagaag
    10321 atcaagaatt ttataagctt ttagctttag ttgatgttat ccgtgtggga aaagtaagag
    10381 aaattgaata tgctgtagat gaactaaaaa aaaatatttt acatgggcca tcatattaat
    10441 aaggtaagaa ttaaagctgt tgccaccgca ttagatactt taaaagaaaa agtagttttt
    10501 gtaggaggtg ctactatttc actttatcca gaccagcctg tttttgaagt caggccaaca
    10561 gatgatgtgg acgtaataat tgaaatacta aattattctg ataggaccga actggaagaa
    10621 aaactgagat caattggctt tgctcacgac atagaatcag gtgtaatatg tcgatatcga
    10681 attcaaggaa ttgtagttga tattatgcca acaaatgatt cttcaattgg ttttactaat
    10741 atttggtatc cagagggtta taaaaacgct gttaattata aaattgatga acgatgtata
    10801 gttaagatat tgagcgcacc atattttata gctacaaaat tagaagctta caaaaatcga
    10861 ggaaaggatg atggaagaac tagtaaagat tttgaggata taatctatgt tttggaaaac
    10921 agagaagtaa tatgggatga aataaataat tcaactgaga atttaaaaaa ttatttgcta
    10981 acagaatttc gaaatctatt aaaaaatcct aatctgtttg aatggataga ttgtcatgtt
    11041 gaaagagggt ctccacctgc ttcttatttc attatggagg aaatagaaaa atttactgca
    11101 taacatttct atctaagagc cattaataat tggcactagt gtattcggga tttgcgttag
    11161 tggccttttg aagcagcaaa ctatttgcca gcaccaatgg ttattaattg ctcaaatttt
    11221 tctttttaca ttatctgcgc acaaacacaa aatcaatatt agctgacatt atttctatga
    11281 tttaacaaat tgcgtcgatt tttcttttat aaacataccg attgttgtaa aaccttttgc
    11341 atttttcttc aatgtttccg ttttcttgtc aaaagtgtgc atgcgtattt ggtcatttgc
    11401 aaaaatattt atttcgtctg tgctgtcgga aacagaaaag tcaaaattgt ttctgttacc
    11461 accaaaagga taaagtgaaa gatgattact cgtcaattcg ttttttggaa tttttacaaa
    11521 tccaattaaa aagtgattat ttcggtgaac gtgcaccacc tatttcggag caaagtgagc
    11581 cacccctt
//
LOCUS       DAORKW010000051        11356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_114_length_11356_cov_5.737368, whole genome shotgun sequence.
ACCESSION   DAORKW010000051 DAORKW010000000
VERSION     DAORKW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11356
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114_length_11356_cov_5.737368"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /gene="mutL"
                     /locus_tag="PLB46_03660"
     CDS             <1..1592
                     /gene="mutL"
                     /locus_tag="PLB46_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRF75643.1"
                     /translation="ARMCFERHATSKIRTAEDIFTKGFRGEAMASIAAIAQVEMKTRL
                     HDAELGTRIVIEGFELKSQEQVQCSGGTNISVKNLFYNVPARRNFLKSNTVELKHIID
                     EFERVALAHPNIFFSFQHNGVEIFHLKAGNHRQRIIHLFGNNYNEKLVPVEENTSVAN
                     IRGFIGKPDSAKKTKGEQFFFVNNRYIRSPYLHHAVMSVYDDLIPENSYPLYTIFIDI
                     DPSRIDVNVHPTKQEIKFEDERIIYTFIQAGVRHALAQYSVTPTLDFNVDNTFANLPS
                     FYKKAEDTVAKHFSFKTIGSKMNDTPKSEKSFFPDLKTVRNDEQQSDFIQQSELDEAP
                     YDGITESAPSFQIHNQYIVAQIKSGFIVIDQQAAHERVLFEKYLYQLQNKKPHTQQQL
                     FPQTLKLSSADAALLSELLSDINNLGFDIQPFGKDAFVIHGVPVEFESGNEQKVVEDL
                     LEQFKEDRSALKLNKHNVIARSMARTNSIKRGQKLDEKGMRHLTDQLFACTTPYVSPF
                     GNLTFTSFSLEELAKQFQKNN"
     gene            1601..2308
                     /locus_tag="PLB46_03665"
     CDS             1601..2308
                     /locus_tag="PLB46_03665"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRF75644.1"
                     /translation="MTQFQPTRFNYLPPVIKNLLIINGLVFLATLAFPGLLEKLALYF
                     PTSPDFQPFQIVTHMFTHADLGHIFFNMFSLWMFGAVLENVWGPKRFLTFYLITGLGA
                     AFLHMGFNAYELYTAVGTLNPDVIITGETISGNYSQHQLQEIISSFAPTVGASGAVYG
                     ILVAFGLLFPNTLIYIYFLLPIKAKYLMIIFIAIEVYMGFVNSPTDNIAHFAHLGGAL
                     FGFLLVKYWGRNKTRFY"
     gene            2312..3202
                     /locus_tag="PLB46_03670"
     CDS             2312..3202
                     /locus_tag="PLB46_03670"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRF75645.1"
                     /translation="MNLTNDIKYQFRYGNAVMKLLLVNVFLFLIQSLVLLVTYILGTK
                     DAYLTFLVKWFWVSSDVSVFITRPWTLFTYMFLHDPLRIFHILSNMIYLYFFGNILAQ
                     FFQHKKIYPLYVVGGIIGFLFAFLIYTLAPNINDGIDMYLVGASAAVMAIVLAAAALV
                     PEYSVSLLLIGPVKLKYIAVVVVIIDLISIPSEMNIGGHLAHLGGALTGYLYIRSYNS
                     GRDWFSWWPRFENKAYNLFEPKKPKVVYRNEQAGPATKKENDITKQAKLDAILDKIKA
                     SGYDSLSKAEKEFLFKISNE"
     gene            3205..4308
                     /locus_tag="PLB46_03675"
     CDS             3205..4308
                     /locus_tag="PLB46_03675"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRF75646.1"
                     /translation="MKWWHKIILFFTILVTIALTGAYLAMYVSPESFWPLAFIGLGYF
                     PILIAYLVFVFIWFFLRKKIFYFLLVLLLLGFKAHLSYFSFGSLKKADTEGKEVITIM
                     QYNVQGFDAYNKAGKYKNRDEIITNIANAKPDILCLEEFNTYHNHPKEKSNMEMVLTA
                     TGLQYHYYYKAFENTRSTRSFGLVIYSRYPIVDTGRLDYLSLSNLNSTIYADIKINND
                     TIRVFCSHLQSTQLSHYDLEFIEASNEAETDFDADRVTDKLKGSFALRSQEADTVAVH
                     IKKSPFPIISCGDFNDTPVSYTYRKMSGDLQDAFLVRGFGIGATYAPFPFIRIDYQLF
                     DKDKFEIVDFKRIKEGSSDHYGCVTKFTINPEQ"
     gene            4389..5891
                     /gene="cysS"
                     /locus_tag="PLB46_03680"
     CDS             4389..5891
                     /gene="cysS"
                     /locus_tag="PLB46_03680"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRF75647.1"
                     /translation="MEQVLFHQNALYVYNTTSRQKERFEPLHAPFVGMYACGPTVYSE
                     PHLGNCRSFVMYDLMFRYMLHLGYKVRYVRNITDAGHVESTSGETGDRISKQAKIEQV
                     EPMEIVQKYSVKFHRIMDTLNCLPPSIEPTATGHIVEQIEMTKKIMENGFAYETDDAI
                     YFDVEKFSANYPYTKLSGRNLDDLLNNTRELGGQESKKGRLDFALMIKAKPEHIMRWP
                     SPWGDVYPGWHIECSAMSEKYLGKTFDIHCGGMDLIPTHHTNEIAQCVACNHVDPVKY
                     WLHPNMLTVNGQKMSKSLGNSFLPAELFDGSHPLLDQGYSPMTVRFSMLQAHYRSTLD
                     FSNDALKAAEKGYKRLMNAKSALDQITISGTVNNEEEANVNKLLLATYDAMNNDFNSA
                     QAIAELNEAASLINTWNSLQNKKANIQEHTLQRFKTLLHHFLFDIFGLLEESENNNEK
                     LNGVMELVIDIRKDARSKKDFATSDKIRDALQGVGIQLKDSKDGTSWQTE"
     gene            6217..7191
                     /locus_tag="PLB46_03685"
     CDS             6217..7191
                     /locus_tag="PLB46_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75648.1"
                     /translation="MKKYFSVLIVLIATNCIISAQSYCVSGNCANGFGKQVMEVGDTY
                     EGDWVEGSRSGKGVYTWVDGSTYVGDFKNDVFHGVGKYTGATGIIIEGYYSNGLYVAP
                     FEQIDYELAEMQQQADSLLEFINNIPEPDYLDEYYDDSYYEEEGYYDEDLSSFDSDSL
                     MAEIYLMIQKDYDVLYAIINDYPTAFNNTKGVEDFSNMFAQQFISTTGFEVAQSNFLN
                     LPMGYKYWQWISVINESADFLVAEANYNYYNSIVEGVSADCCTLVASTKEVKDNYNES
                     YTTTYTPSIIADGYSSEYANMQIIVSMYKNYNNLSDYTITVSIRDARN"
     gene            complement(7343..8467)
                     /locus_tag="PLB46_03690"
     CDS             complement(7343..8467)
                     /locus_tag="PLB46_03690"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRF75649.1"
                     /translation="MKKVIVLGAGMVGRAMAIDLAKDYAVTSADISEENLSRLKAFGI
                     ETIQADLTNKEKIQSLIADKDLVIGAVPGFMGFETFKTVIESGKNTSDISFFDEDPFL
                     LHDMAVKNNVTAVMDIGVAPGMDNIILGYHNKRMEVTDFVCLVGGLPAARIWPYEYKA
                     PFSPIDVLEEYTRPARFVKDKKVVIKPALSEPELMDFNEIGTLEAFNSDGLRSLIKTM
                     PNIPNMIERTLRYPGHIELMRVMRETGFFSKEEIMVKGVLIRPLDVTAKLLFPKWKLG
                     DEENEFTVMRVIVAGKENGVQKTYTYDLLDRYDAATKTSSMARTTGYTCTGAARLILE
                     NKFTRKGVSTPEFVGEDENNFHDMLNYLKARNIHYKLTIA"
     gene            complement(8600..>11356)
                     /locus_tag="PLB46_03695"
     CDS             complement(8600..>11356)
                     /locus_tag="PLB46_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75650.1"
                     /translation="VCSGDSIQLTAITAATGGYLWSTGSVINPIYVTASGNYSVTVTD
                     AAGCTASANINATINPLPDLSMLPIGCDSACINPDPDTIHGPPGMLSYNWLINNITAS
                     TAQDLILNTTNMPVFGIPYTVTLIATTTFGCVDTASFEYTPIDCDSASCFDLIDSIYC
                     NPDGTFTLNLVITNNNAATSTYLWLHDINAPFTFTPTFVIPLTLTQGQTSAPIPIIVS
                     FPPGPLPTDICFKASLYNTDSCCHDSVLYCIPVPDCSPCGNVSVSMSSDSTDECCKTI
                     DITNNYASNYFSGVQFVPISTGTSIASAQLGLPYLGSWGGMGNSTQMTFTPNSGFIPT
                     GTTAGLVELCLNLTPAAPSPQIVIVNWFVSTPLGDSIACTDTLLFYCDAPLENPCGDI
                     HGEITCVGDGTYQYDFTFTNNSTHNVTMLVFTNTNPVLNVIPNPIILGSPVVPGDSIT
                     SSVIIDPGIWPPGTDFCFDLTLADATGWCCHEMDSICITLPECDTCACGNWDLFTMGT
                     SDGLEVFVECGINISIVNPGTTVTFLTGGYNCIGDSTCVGEIAWTLTGPVTTSGTGLP
                     TFTVTTGGVYTLTMIGSCDGNACDTCIITFTVPEICECGGGWQSFGMNVDVPGALDYS
                     ILADCGELFSSIPVGTVISAVSGGYLCNGDPAACPALIKWSLTDPLGNSITGVGYPTF
                     TTSLPGTYSLTFSAVCNGQKCGTCNIKFSVVDVALCDCGTWGPITVKKGTKNLKNQPC
                     GTGYNWTTNTPIIISGAYTCAGPADCLTTYSWTATRNGVFYTSGTSMPVNFTPTLNGT
                     YVVTITPICNGVECPPCEFTFKAKTPIAKEGNVELESLFNMRIAPNPASTNVKVTVED
                     ATDNFGNIQIVNELGVVVLESEIRFEYFNTIELNIELLPAGCYMVRYIGENESQIQQL
                     VIVK"
BASE COUNT         3691 a         1987 c         2078 g         3600 t
ORIGIN      
        1 atgccagaat gtgtttcgag cggcatgcaa cgagcaaaat tcggacggct gaggacattt
       61 ttaccaaggg tttcagggga gaggctatgg ccagtattgc tgccattgcg caggttgaaa
      121 tgaaaacccg acttcacgat gccgaattag gaacaagaat agtgattgag gggtttgaac
      181 tcaaatcgca ggaacaagtg cagtgttcag gtgggacgaa tatttcggtg aaaaacctgt
      241 tttacaatgt tccggctcgt cgtaacttct tgaaatccaa tactgtagag ttaaaacata
      301 ttatcgatga gtttgagcga gtagcattgg ctcatcccaa tatatttttc agcttccagc
      361 acaacggggt tgaaatattt catttaaaag ccggcaatca tcggcaacgg attattcacc
      421 tttttggtaa taattataat gaaaagctgg ttccggttga ggaaaatacc tcggtagcca
      481 atattcgcgg atttattgga aaacccgatt ctgccaaaaa gaccaaaggc gaacaattct
      541 tttttgtcaa taaccgctat atcagaagtc cgtatttgca tcatgctgta atgagtgttt
      601 acgatgattt gattccggaa aacagttatc cattgtacac aatatttatc gatatagacc
      661 cgtctcgtat tgatgtgaat gtgcatccca ccaagcagga aattaaattt gaagacgagc
      721 gtattattta tacgtttatt caggcaggtg ttcggcatgc actggcacaa tacagtgtta
      781 ccccaacttt agattttaat gtagacaata cgtttgccaa tttgcccagt ttttataaaa
      841 aggctgagga cacggtcgct aaacattttt cgtttaagac cattggcagt aaaatgaatg
      901 atacacccaa atcggagaaa tcgttttttc cggatttgaa aacggtgaga aacgacgagc
      961 agcaaagtga ttttattcag cagtcagaat tagatgaagc accttacgat ggtattaccg
     1021 aaagtgctcc ttcgttccaa atacataacc agtatattgt tgcacaaatt aaatcagggt
     1081 ttattgtaat tgaccaacag gcagcacatg agcgggtgtt gtttgaaaaa tatttatacc
     1141 agttacaaaa taaaaaaccg catacacagc agcagttatt tccgcaaacg ctaaaacttt
     1201 catcggcaga tgctgcattg ttaagtgaac ttttatcgga cataaacaat ttaggatttg
     1261 acattcagcc atttgggaaa gatgcatttg tgatacacgg tgttccggta gaatttgaat
     1321 ccggtaatga gcaaaaggtt gtagaagatt tacttgaaca attcaaagaa gatcgttcgg
     1381 cacttaaatt aaacaaacac aatgtaattg cccgatcaat ggcgcggaca aatagtatta
     1441 aaagaggaca aaaattagat gaaaaaggca tgcgccattt aactgaccag ttatttgcat
     1501 gcaccacacc atatgtttcg ccatttggta atttaacttt cacctcattt tcattagaag
     1561 aattagcaaa acaatttcag aaaaataatt aacaggcaag atgacgcaat ttcaaccgac
     1621 aagatttaat tacctccctc cggttataaa aaacttactc ataattaatg gtttggtgtt
     1681 tttagcaacc ttggcatttc cagggttatt ggaaaaactt gcattgtatt ttcctacttc
     1741 acctgatttc caaccatttc agattgtaac gcatatgttt acacatgctg atttgggaca
     1801 tatctttttt aacatgtttt cgttgtggat gtttggcgca gtattagaaa atgtttgggg
     1861 accaaaaaga tttcttacat tttatttaat taccggatta ggcgctgctt ttttacacat
     1921 gggatttaat gcttacgaat tatatactgc agttggcact ttaaatcctg atgtaattat
     1981 taccggtgaa accatatctg gaaattattc gcaacatcaa ttacaggaaa tcatttcatc
     2041 gtttgctcct acagttggtg cttcaggagc ggtttatggt atattggtgg catttggatt
     2101 gttgtttccc aacacgctca tttatattta tttcctattg cctattaaag caaagtacct
     2161 catgataatt tttattgcca tagaggtgta tatgggattt gtaaacagcc ctaccgataa
     2221 cattgcgcac tttgcacatt taggtggagc attatttgga ttcttattag taaaatactg
     2281 ggggcggaac aaaacgcgtt tctattaaat aatgaatctc accaacgaca ttaaatacca
     2341 attcaggtat ggcaacgcag ttatgaagtt gttgctggtg aatgtatttt tatttctcat
     2401 tcagtcgctg gtgctattag taacttatat acttggcacc aaagatgctt atcttacttt
     2461 tttagtgaaa tggttttggg taagttcaga tgttagcgta tttataacac gcccctggac
     2521 attattcact tacatgtttt tgcacgaccc gctaagaata tttcatattt tatcgaatat
     2581 gatttattta tattttttcg ggaacattct tgcgcagttt tttcagcata aaaaaatata
     2641 tccactatat gttgtaggtg gcattatagg atttttattt gcatttttaa tttatacact
     2701 cgctcccaat attaatgatg gcatcgatat gtatttggtt ggcgcttctg ctgctgttat
     2761 ggcaatagta ttagcagccg cagcattagt tccggaatat tcggtatcgc ttttattaat
     2821 aggtcctgtc aaactaaaat atattgccgt tgtggtggtt attattgatt taatcagtat
     2881 tcccagcgaa atgaatattg gcggacattt ggcacatctt ggtggtgctt taactggata
     2941 tttatatatt cgcagctata attccggtcg cgattggttt agctggtggc caaggtttga
     3001 aaacaaagca tataatttgt ttgaaccaaa aaaaccaaaa gtggtatatc gcaatgaaca
     3061 agccggccca gcaacaaaaa aagaaaacga tattactaaa caggctaaac ttgatgcaat
     3121 tttagataaa ataaaagcat ccggttatga tagtttgagc aaagcagaaa aagaattttt
     3181 atttaagata agtaatgagt aacaatgaaa tggtggcata aaatcatatt attttttaca
     3241 atactcgtca ccattgcatt gacaggtgct tatttagcca tgtacgtaag tcctgaaagt
     3301 ttttggccac ttgcatttat cggattagga tatttcccta tattaatcgc ctaccttgtt
     3361 tttgtattca tatggttttt tctacgcaaa aaaatctttt attttttatt ggttttatta
     3421 ttgctgggtt ttaaagccca tctaagttat ttttcgtttg gttcgttaaa aaaagcagac
     3481 actgaaggta aagaagtaat caccattatg caatataatg tgcagggatt tgatgcttat
     3541 aataaagccg ggaaatataa aaacagggat gaaattatta ccaatattgc caatgcaaaa
     3601 cccgatattt tatgtttaga agaatttaat acctaccata atcatcctaa ggaaaagagc
     3661 aatatggaga tggtattaac tgccacaggt ttgcaatatc attattatta caaggcattt
     3721 gaaaacacca gaagcacacg tagttttggg ttggttattt attcgcgcta tccgattgta
     3781 gacacaggtc gtttagatta tttatcatta agcaatttga attccaccat ttatgctgat
     3841 ataaaaataa ataatgatac cataagggta ttttgttctc atttgcaaag tacacaatta
     3901 agtcactacg acttagaatt tattgaagca agcaatgaag cagaaaccga ttttgatgct
     3961 gaccgcgtta ctgataaatt gaaaggcagt tttgctttgc gttcacagga agctgatact
     4021 gttgctgttc atattaaaaa atcaccattt ccaattattt catgtggtga ttttaatgat
     4081 acacctgtgt catatactta tagaaaaatg agtggcgatt tacaggatgc atttttagta
     4141 cgtggatttg gcattggcgc tacatatgca ccgtttccat ttatcagaat tgattatcaa
     4201 ttattcgata aagacaaatt tgaaattgtc gattttaaaa gaattaaaga gggctccagc
     4261 gatcattatg gttgtgtgac aaaatttacc atcaacccgg agcaataatt cactttcagg
     4321 accagcgtct tcgcataaaa attacctaac aacttcatca ttttactttt acgcgctaac
     4381 tttaccacat ggaacaagtg ttatttcatc aaaacgcctt atacgtttat aatacaacaa
     4441 gtcgccaaaa ggagcggttt gagcctttac atgcaccatt tgtaggcatg tatgcctgcg
     4501 ggcctacagt ttacagtgaa ccacatttag gcaactgcag gagttttgta atgtatgatt
     4561 taatgtttcg ctacatgctg catcttgggt ataaagtgcg atatgtacgc aatattaccg
     4621 atgcagggca cgtagaaagt acaagtggtg aaaccggaga ccgcatcagc aaacaagcta
     4681 aaatcgaaca ggtggaaccg atggaaattg tacagaagta ttctgtaaaa tttcatcgca
     4741 ttatggatac acttaattgt ttacctccca gtattgaacc tactgcaacc ggacatattg
     4801 ttgagcaaat agaaatgaca aaaaaaatta tggaaaacgg tttcgcttac gaaactgatg
     4861 atgccatata ttttgatgtc gaaaaatttt ctgccaatta tccttatacc aaattaagtg
     4921 gcagaaatct ggatgatttg cttaataaca cccgggaatt aggtggtcag gaaagtaaaa
     4981 aaggtcgatt ggattttgct ttaatgataa aagcaaaacc agaacatatc atgcgttggc
     5041 cttcaccttg gggagatgta tatccgggct ggcatattga atgttcggca atgagtgaaa
     5101 aatatttagg taaaactttt gatattcatt gtggtgggat ggatttaatt ccaacacatc
     5161 atacgaatga aattgcacag tgtgttgctt gtaatcatgt tgaccctgtg aaatactggt
     5221 tgcatccaaa tatgcttaca gttaatggcc aaaaaatgag taaaagttta ggcaattcat
     5281 ttttacctgc agaattattt gatggttctc atccattgtt agatcagggc tattcgccaa
     5341 tgactgttcg cttttcgatg ttacaggcac attatcgcag tacattggat tttagtaatg
     5401 atgcactaaa agcagcggaa aaaggctaca agcgattaat gaatgctaaa tcagcacttg
     5461 atcaaattac tatttcagga actgtaaata atgaagagga agccaatgta aataaattat
     5521 tattagctac ttacgatgct atgaataatg actttaactc ggcacaagct atagctgaat
     5581 taaacgaagc tgcatccttg ataaatacct ggaacagttt acaaaataaa aaagctaata
     5641 ttcaggagca tactttacag cgttttaaaa cattattaca tcatttcttg tttgacattt
     5701 ttggattatt ggaagagtcg gaaaacaaca atgaaaaatt aaacggcgta atggaattgg
     5761 ttattgatat cagaaaagat gcacgcagca aaaaagattt tgcaaccagt gataaaattc
     5821 gagatgctct tcaaggtgtt ggtattcaat taaaggatag caaggatggc acaagttggc
     5881 aaacggaata aaggtaatta attcataata aaagcgatca gctgcaaagt tggtcgcttt
     5941 tttatttgtt acaaattacc attattctaa acatttttca ccttacttat tttatccgtt
     6001 ttatattcga ttatgtctat cagtaatttg cttgactatt aaaatcagaa aatggaatgt
     6061 tattattcaa atgaacgata agtctgtgga atgccggaaa aacactgttg aatcggagat
     6121 cctttagcac atgcataaaa taataaacgg gattagttaa ttattagctt aacttagcat
     6181 aaattcaatg gcatagtcag ccaataaaaa taatttatga aaaaatattt ttcggtttta
     6241 atagtactta ttgcaactaa ttgcattata agtgcgcaat cctattgtgt ttccggtaat
     6301 tgtgccaatg gttttggaaa acaagttatg gaggtaggag atacttatga aggcgattgg
     6361 gtagaaggct caaggtcagg gaaaggtgtt tacacctggg ttgacggttc aacatatgtt
     6421 ggcgatttca aaaatgatgt ttttcatggc gtagggaaat atacaggagc cacaggaata
     6481 attattgaag gatattactc caatggttta tacgtggcac catttgaaca aattgattat
     6541 gaacttgctg aaatgcaaca acaggctgat agtttgcttg aatttataaa taatattcca
     6601 gagccagatt acttagatga atattatgat gatagctatt atgaagaaga ggggtattat
     6661 gatgaagatc tatctagctt tgacagcgat tcattaatgg cagaaattta tctcatgatt
     6721 caaaaagatt atgatgtgct gtatgcaatt attaacgatt atcctactgc gtttaataac
     6781 acaaaaggcg tcgaagattt ttcgaatatg tttgcacaac aatttatttc aactacaggt
     6841 tttgaagttg cacaaagtaa ttttttaaat cttcctatgg gttacaaata ttggcaatgg
     6901 attagtgtca taaacgagtc tgccgatttt ttggtagctg aagcaaatta caattattat
     6961 aattccatag ttgaaggagt atctgcagat tgttgtacat tggtggcaag cactaaagaa
     7021 gttaaggaca attataatga atcctatact acaacttaca caccatcaat tattgccgat
     7081 ggatattcat ctgaatatgc taatatgcaa ataattgtta gtatgtataa aaattataat
     7141 aacctgtcgg attacaccat aacagtgagt attcgggatg cgaggaatta gtaaaattaa
     7201 ttcaacgctt ggattggcat ttcaaaaagt taattcattt gaatatttat acctattgct
     7261 tatttacaag taacttaata agttacactc aacctaactt tcgaattaat ttggtatctt
     7321 gtttttactc aagtagtcaa tcttaagcaa tcgtcagctt ataatgtata tttcgagctt
     7381 ttaagtaatt gagcatatcg tgaaaattgt tttcatcttc cccgacaaat tccggtgtgc
     7441 ttacgccctt tcttgtgaat ttattttcga gaattaaacg ggcagcacct gtgcaggtgt
     7501 aacctgttgt acgagccatt gatgaagttt tggttgctgc atcgtaacgg tctaacaaat
     7561 cgtaagtgta tgttttttga acaccatttt ctttaccggc aacaattact ctcattaccg
     7621 taaattcatt ttcttcatca cctaatttcc attttggaaa taacaattta gctgttacat
     7681 ctaatgggcg aatgagtacg cctttaacca taatttcttc tttactgaaa aatccggttt
     7741 cgcgcattac tctcattaat tcaatatgcc cggggtatct taaagtgcgc tcaatcatat
     7801 taggaatatt cggcattgtt tttattaacg aacgcaaccc atcagaatta aatgcttcga
     7861 gtgtgccaat ttcattaaaa tccattaatt ccggttcact gagtgccggt ttaataacta
     7921 cttttttatc ttttacaaat cttgcagggc gtgtatattc ttccaacacg tcaattggag
     7981 aaaacggtgc tttatattca taaggccaaa tgcgtgctgc aggtaaaccc ccgactaaac
     8041 atacaaaatc ggtaacctcc attcgtttgt tgtggtaccc taaaataata ttatccattc
     8101 ccggagcaac accgatatcc attacagcgg taacattatt ttttaccgcc atgtcatgca
     8161 ataaaaaagg atcctcatca aaaaaagaaa tatcgctggt gtttttaccg ctttcaatta
     8221 ctgttttaaa tgtttcgaaa cccataaaac cgggaacggc accaatcact aaatctttat
     8281 ctgcaataag tgattgaatt ttttctttat tggttaaatc agcttgtatt gtctcaatac
     8341 caaatgcctt taatctgctc aaattttcct cactgatatc cgcagatgtt accgcataat
     8401 ctttagctaa atcaatagcc atagcgcgtc ctaccatgcc tgccccaagt acaattactt
     8461 ttttcatatt aaatgcttta caataagttg attatcaaat tcaatcacaa aggtaaaact
     8521 aatttattca gcaataaaaa aactcccgca attacaataa ctgcgggagt tccaagcttt
     8581 ataatagtga attatgtggc tattttacaa tcactaattg ctgaatttga gattcatttt
     8641 caccaatata gcgaaccata taacaaccgg caggcagcaa ttcaatattt aattcgatag
     8701 tattaaaata ttcgaatctg atttcgcttt ctaaaactac tacgccaagt tcatttacaa
     8761 tttgaatgtt accaaaatta tctgttgcat cttcaactgt tactttaaca tttgtagagg
     8821 cagggtttgg tgctattcgc atattaaata atgactctaa ttctacattt ccttctttag
     8881 caataggtgt ttttgctttg aaagtaaatt cacaaggcgg acattccact ccattacaaa
     8941 ttggagttat agtaactaca taagtaccgt ttaatgtagg tgtaaagtta accggcatgg
     9001 aagttccact tgtatagaac acaccatttc ttgttgctgt ccatgagtag gttgttaaac
     9061 aatcagctgg accggcacaa gtatatgctc cggaaattat aattggtgta tttgttgtcc
     9121 agttatagcc ggttccacat ggttggtttt tgaggttttt agttcctttt ttaactgtaa
     9181 ttggacccca agttccacaa tcgcaaagag ctacatctac aacactaaat ttgatgttac
     9241 atgtgccaca tttttgacca ttacaaacag ctgagaatgt gagcgaataa gtacccggta
     9301 aacttgttgt aaatgttgga taaccaacac ctgtaatact attaccaagc ggatcggtta
     9361 acgaccattt tattaatgct gggcaagctg caggatcacc attacacaag tatccacctg
     9421 acacagcgct tattactgtt cctacaggaa ttgatgagaa taattcaccg caatcggcaa
     9481 gtattgaata atctaatgcg ccaggaacat ctacattcat tccaaacgat tgccatccac
     9541 caccacattc gcatatttca ggcactgtaa atgtaataat acatgtatca catgcattac
     9601 catcacaaga accaatcatg gttaatgtat atactccgcc tgttgtcaca gtaaaggttg
     9661 gtaatcccgt tccggaagta gtaacaggtc cggttaaagt ccaagcaatt tctccgacac
     9721 aagttgaatc tccaatacaa ttatatccac ctgttaaaaa tgtaaccgtg gttcctggat
     9781 taactataga aatattaatt ccacattcaa caaacacttc taatccatca ctagtaccca
     9841 tggtaaataa atcccaatta ccacatgcac aagtatcgca ttccggtaag gtaatacata
     9901 tactatccat ttcgtgacaa caccatccag ttgcatcggc taatgttaaa tcgaaacaga
     9961 aatcagttcc cggaggccaa atacccggat caataattac acttgatgta attgaatccc
    10021 ctggaactac cggtgaacca agaataattg gatttggaat tacatttaat accggattcg
    10081 tatttgtaaa taccaacatg gtaacattat gtgtactgtt atttgtaaag gtaaaatcat
    10141 actgataagt accatcacca acacaagtta tttcaccatg aatatcacca caaggatttt
    10201 ctaatggcgc atcgcaataa aataataagg tatcggtgca ggcaatacta tcgccaagtg
    10261 gtgttgatac aaaccaattt acaattacaa tttgcggact tggtgcagcc ggtgttaaat
    10321 tcaaacacaa ttcaaccaaa cctgcagttg ttcctgtcgg aataaatcct gaatttggtg
    10381 tgaatgtcat ttgtgttgaa ttacccattc caccccatga tccgagataa ggtaatccta
    10441 attgtgcaga ggcaattgaa gttccggttg aaatcggcac aaattgaact ccggaaaaat
    10501 aattagaagc ataattattg gtaatatcaa ttgttttaca acattcatca gtagaatcgg
    10561 agctcatgct tacactaaca tttccgcatg ggctgcaatc aggtaccgga atacaataca
    10621 acacgctatc atgacaacag gagtcggtat tatataacga agctttaaaa caaatatctg
    10681 ttggcagtgg tcccggaggg aatgaaacaa taataggaat aggcgcagat gtttgtcctt
    10741 gggttaatgt caacggaatt acaaatgttg gtgtaaatgt aaatggtgca ttaatatcgt
    10801 gcagccataa ataggttgac gttgcagcat tattattggt aattacaaga tttaatgtaa
    10861 atgtgccatc cggattacaa taaattgaat cgattaaatc aaaacatgaa gcactatcac
    10921 aatcgatagg tgtatattca aatgatgctg tatctacaca accaaaagtt gtagttgcaa
    10981 ttaatgtaac cgtatatgga attccaaata ccggcatatt agtcgtattt aaaattaaat
    11041 cctgagcagt acttgcagta atattattta ttaaccaatt gtaagacaac attccaggag
    11101 gaccgtgaat ggtatccggg tcaggattaa tacatgcaga atcgcaacca attggcaaca
    11161 tagataaatc tggtaaagga tttatagttg cattaatatt agctgaagca gtacaaccgg
    11221 cagcatctgt tacagtaact gaataattac cacttgcagt aacataaata ggatttatta
    11281 cacttcctgt gctccacaaa tatccacctg ttgcagcagt aattgctgtg agttggatgg
    11341 aatcacctga acaaac
//
LOCUS       DAORKW010000052        11349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_115_length_11349_cov_5.996812, whole genome shotgun sequence.
ACCESSION   DAORKW010000052 DAORKW010000000
VERSION     DAORKW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11349
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115_length_11349_cov_5.996812"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..736)
                     /locus_tag="PLB46_03700"
     CDS             complement(137..736)
                     /locus_tag="PLB46_03700"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRF75651.1"
                     /translation="MINLILFGPPGSGKGTQAEKLKAHFNLLHISTGDLLRAEIAGGT
                     QLGLAAKQFMDEGKLVPDEVVIGMLGGAVEEAKSKGKQGIIFDGFPRTSAQAQALDNM
                     LLEKGTSVSCVLSLVVDEEELTQRILKRGLTSGRSDDNDVETIRKRVQEYRTKTEPVA
                     GYYKNQDLLIEIEGVGTIDTIFDALMQAVNSVSSKHSDN"
     gene            892..1182
                     /locus_tag="PLB46_03705"
     CDS             892..1182
                     /locus_tag="PLB46_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRF75652.1"
                     /translation="MNVLEEIKETLTKNKPYLSSKYSLSSIGLFGAVVKDDFIPGSEI
                     DIIVDFKKPIGIEIIDLTDELEKLLKHKVDLVAKNGIEKGYYKVFRKEILYI"
     gene            1255..1674
                     /locus_tag="PLB46_03710"
     CDS             1255..1674
                     /locus_tag="PLB46_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75653.1"
                     /translation="MQVFKLLVGIVLVFFTVMFMRCEKVTEISELIKNTQKIQVVFYN
                     DTLPDTFVDITDKKDIKKFKNYITIEDTPIYKCGYDGQLIFFMDDEEGAQPKNSVAME
                     FNLNPECMHIAYQYAGALQTKKLSEEGINYLLSLQNK"
     gene            1674..2423
                     /locus_tag="PLB46_03715"
     CDS             1674..2423
                     /locus_tag="PLB46_03715"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HRF75654.1"
                     /translation="MLRMPVYDSRETNFPVLSAIILSNLMPLIAVLFYDVSFFALFYL
                     YWWETVIISVFQFIKMGKAQKLSEPDPNFSINDKTLSYEQVNSKRYMRTSYVVIRFLM
                     LVFYLFFIVIFVGVLSTMQENPTAFVRALLFTEPWVKVSFAGFFVTHLVEYIFWRSNK
                     EYEQTSLRELGTVFDTRVMVIHIVIVLGTFAAMFAGDKLMPGAQNAGSIAYVCLFVLM
                     KIAMDVYAHNKSTRRLEVINALTNSLISKKP"
     gene            complement(2525..3052)
                     /locus_tag="PLB46_03720"
     CDS             complement(2525..3052)
                     /locus_tag="PLB46_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRF75655.1"
                     /translation="MQIRNKTFKLYLDANTIQKRIAEMGKEITADFHDKNPVFIIVLT
                     GAFAFSSELIRQFQGKCVVRFTRIKSYTGTVSGEIETFTGFDIPIKDRHVIFLEDIID
                     TGKTVFHLTKEAEKLKPASITVATLLQKQILRPNLIKGDYVGFEIPDVFVVGYGLDYD
                     EEGRNWNGVYQLVAE"
     gene            complement(3059..4444)
                     /locus_tag="PLB46_03725"
     CDS             complement(3059..4444)
                     /locus_tag="PLB46_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRF75656.1"
                     /translation="MKGLIESGLKHTGIAIVLFLLSASVLAGQPNQKVLDYIDLYKEI
                     AVREMIEYKIPASITMAQGILESGAGQSELALKSNNHFGIKCHTDWKGDKVYYDDDAK
                     DECFRKYDHVEESYRDHSTFLVNKTRYAELFTLSITDYKGWAKGLKAAGYATNPKYAD
                     LLINLIEEYNLDELDKMTLADIKKQDKKTDKKQNDPIVKEDKPVENKQPKKVSWGGYN
                     EEVYYFNRIPTITVEAGDSPESLAKKHHIKIHLLKDYNDIENGGELTPGTKFYLQPKR
                     KKGDTKYHIVKEGENMWSISRDEGVRLSQLYKYNKISEGQEPAVGESINLRSKRHNDL
                     KLAGKTPTKKPVEVKQQKQEEKQPAPKQEKEPEFKDDNNDVFIDYEEEIIAPEVKDPK
                     AEKEQDIYTPDEPKKEAPVALPIYHIVAAQETLYAISKKYQVTVAQIQSWNNITDTNI
                     KIGQQLIVGYK"
     gene            complement(4511..5152)
                     /locus_tag="PLB46_03730"
     CDS             complement(4511..5152)
                     /locus_tag="PLB46_03730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRF75657.1"
                     /translation="MDDLISDALSAYIDKYTTAEDTHLSALNRETHLKAMMPRMLSGH
                     VQGKFLEFISCMIKPAHILEIGTFTGYSAICLAKGLRQNGQLITIDINEELTPMVKKY
                     VAAENMEDKIAIRTGNAMTIIPTLTQQFDLVFIDADKTNYANYFDLVIDKVAPGGWIL
                     ADNVLWSGHVTDAKHDKDTAAIHAFNEKVHLDPRVEQVIVSIRDGITIARKNG"
     gene            5227..6810
                     /locus_tag="PLB46_03735"
     CDS             5227..6810
                     /locus_tag="PLB46_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HRF75658.1"
                     /translation="MKFYFTLIIVLLGINESAFAQYNIAYAGKVDYADTIQLSGCWGY
                     TADDGKEYALVGTTHGTSIVDVSNPLLPEELFFVPGSLSTWREVKTWNKHAYVTTEGG
                     GGLMIIDLQNLPDAIDTISYHGTVDHPFKTAHTLIFDEFGFGYLFGFNILTGANEGAY
                     IIDVNVDPKHPEFVAEFTDVYVHDGFVRDNQLWAAAIYEGGVYVYDVTNKNDFTLLGV
                     QPTSASATHNCWLSDDGNYLFTTDEIWTGFITSFDVSDVTDIKTLDQIKHGAADSTVA
                     HNTYYLNGYLVTAHYTEGVTIHDAQRPGNLIEVGHYDTSPFGPEVLLEGAWGVYPYFP
                     SGTIIISDIGEGLIVLQPTYTEAAFLEGTVKDEVSFGNIIHALIEISGTPVVDSTDIL
                     GVFETGYHEAGIYDITISKSGCVTKTITDVALINGEVTNLDITLDCGTLSIGELPENK
                     INCYYLPDNGIIYVNNQSDLSNHVNGRLINSEGKIIQEISLAQSATLQLDANALPKGL
                     YYFEFYTAETTFTKKLMLY"
     gene            6935..7960
                     /locus_tag="PLB46_03740"
     CDS             6935..7960
                     /locus_tag="PLB46_03740"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HRF75659.1"
                     /translation="MRSLLFCCFVVSSILVKAQILPAYGDSRTATTGWQFLKIAPDGR
                     SAAMSESFLAVVDDVSSLYWNPAGLTQLDTNKFNFAVDYTDFAAGTSLNFAGITYRIN
                     DNTLVGVSMQYFDAGEMDVTTEFLPFGTGQTFRATDLGLGVSLAKELTDQFSFGVTAK
                     YVREDLASVHNQNVVFDFGFQYDIGIQNTRFAVAISNFGFNTQPGGEIVVLNLSNDST
                     VNEFTEIAVPTVFRLGFAWDAIKNDKHILTTALQLNHPTDNNETYSIGVEYGWKHIFY
                     ARTGYTFATDSGAFPAFGFGTWINRRFGQIKLDYGFNYLTNLGMINKIGIAYTLPNSF
                     KQSNERQ"
     gene            7961..9184
                     /locus_tag="PLB46_03745"
     CDS             7961..9184
                     /locus_tag="PLB46_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75660.1"
                     /translation="MKKLYLGITTVFVMLILSGCDFLFGTREDSTVDEIFEEGAIDPD
                     IIQNEAGYVPILPFWNEFVNPTDIFCGYDEMIYVVDDEGLKVLDQTGTVYNTFYIQGA
                     TDVTQDRRLHTYVCGRVDIDVDNDGTTENLAAVYHLTGTSSGAIQIVDTLIHPFCDVS
                     RNVTSFRGAEDEAVEFTGVTTLADNTVYVSRKGPNNDIAGIARTDNAVLFYNADGTNI
                     GYANGLSPTTSNLRSVWDITGIATFAAPPQSLSGISSSPEFIVTLNNPAAQYKCLWIA
                     QFSDPESGITYGENAALTVFDYTKADQFLYEPNRFINPADVYIAPDFTGYIFVVDEGT
                     DSLYQFTRKGYEGVNPPAGSSTTKQIIASFGGEGSGPFNFINPSGVAYLRETVYVVDR
                     GNNRICRYKLSTDLE"
     gene            complement(9187..10110)
                     /locus_tag="PLB46_03750"
     CDS             complement(9187..10110)
                     /locus_tag="PLB46_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF75661.1"
                     /translation="MQTILGSGGTIGKDLAKELLSYTKDIRLVSRNPKKVNATDTLHP
                     ADLSDPKQIDTAIAGSEVVYVVVGFEYSLKVWQQNWPTLMRHVIDSCERHNCKLVFFD
                     NVYSYAKSAVPHMTEDAPLDPPSKKGIVRKQLVDMIWDAVKHNRINAIIARSADFYGP
                     GNDKSMLVTTVAENFKKGKKANYFGGVNYIHTFTYTPDAAKATAILGNTEDAYNQTWH
                     VPTTSEKLTGKQWIELFANEMHVKPDVMILPVWMMKLLGLFMPIMKEFPEMNYQYEQD
                     YILDSSKFEKRFGIKATSPAEGVKNTVAAMK"
     gene            10367..>11349
                     /locus_tag="PLB46_03755"
     CDS             10367..>11349
                     /locus_tag="PLB46_03755"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75662.1"
                     /translation="MQLTTEPGSFSAMPFKLNESIPLSHLKVWVLAPHLETNDPNLDY
                     YYDFTQSIAEYTKTFEEIGVNWIWQPVTMQSYVTVIEQIVTERNAGINFPIVFNICDG
                     DEVNGTPGVSVVNLLEAKGLIYTGADAYFYNITTSKITMKEAFDLAGVPTAKWEAITN
                     PNQDITGIFERLGAPLIVKPAVSGGSMGVCIKNVVNNEAELKEQITKMFDGYRGWHLS
                     SGGVVVEEFINGPEFTAMIVGSSDQQKNMQVYEPVERIFHEALPDNEKFLSFDRLWEI
                     YEDETPMPDNGNFYQYAAAPAALNRKIKKISWDAYVSNGGKGYTRVDLRMD"
BASE COUNT         3394 a         2035 c         2100 g         3820 t
ORIGIN      
        1 atggtgtttt tagctctatg gaaatgcacg cttcctgcac cacccttacc gcttttgcag
       61 tggattttta cgtaatcaac aaaattctgc ttctccattg tatacctgat attataaacc
      121 tgaaaacggg tagatgtcag ttatcgctat gctttgatga aacgctgtta actgcctgca
      181 tcagcgcgtc aaaaattgta tcgatagtgc ctacaccttc aatttcaatc agcaaatcct
      241 gatttttata gtagcccgca accggttctg ttttggtgcg atattcctgc acgcgttttc
      301 tgatagtttc cacatcatta tcatcacttc ttccgcttgt taaaccgcgt ttcagaatgc
      361 gctgtgttag ttcttcttca tcaaccacca gacttaacac acaactcacc gatgtgcctt
      421 tttctaacaa catattgtct aaagcctgcg cctgggctga agttcttggg aaaccatcaa
      481 atataatccc ctgttttccc ttactttttg cttcttcaac cgcaccaccc aacatgccaa
      541 tcactacttc atcaggcacc aatttgcctt catccataaa ttgttttgct gctaagccca
      601 actgtgtgcc gccggcaatt tctgccctca gcaaatcgcc ggttgatata tgcaaaagat
      661 taaaatgtgc tttgagtttt tcagcctgag tgcccttgcc gcttcccgga gggccaaaaa
      721 gtattaaatt gatcatgaaa aaaataaatt gaagctgcaa agatagcggg ttatggcgga
      781 tttatcgcac aaaacgccca ttatctctgt tttaggtaaa taacgaagac ggagattagc
      841 catccaagtt atttttaaga tgagttctat aatatcggtt acctttgctt tatgaacgta
      901 ctcgaggaga taaaagaaac actcaccaaa aacaaaccgt acctttcatc aaaatacagc
      961 ttaagcagta taggattgtt tggggcagtt gttaaggatg attttattcc cggcagtgag
     1021 attgacatta ttgttgattt taaaaaacca attggtattg aaataattga cctcactgat
     1081 gaattggaga agttgttaaa acacaaggtg gacctggtag caaaaaatgg tattgaaaaa
     1141 ggctattata aagttttcag gaaggaaatt ttatatatct aagcctgaat ttttgagttt
     1201 ttgaccatta gataaagcag caaattactt tttaccgtaa ctttagcatt cattatgcaa
     1261 gtatttaaat tattagtagg cattgtattg gtatttttca ctgtcatgtt tatgcgctgt
     1321 gaaaaggtta ctgaaatttc ggagctgata aaaaataccc aaaaaattca ggtagtattt
     1381 tacaacgata cgctacctga cacctttgtt gatattaccg ataaaaagga tatcaaaaaa
     1441 tttaaaaatt atattactat agaagatacc ccgatttata aatgtggtta tgacggacag
     1501 ttaatatttt ttatggatga tgaagaagga gctcaaccta aaaattcagt ggctatggaa
     1561 tttaatctga accctgaatg tatgcatatt gcttatcaat atgccggtgc attacaaaca
     1621 aaaaaattaa gtgaggaagg gataaattac ttactttcat tacaaaataa ataatgcttc
     1681 gaatgccggt ttacgattca agagaaacaa attttccggt tttatccgca attattcttt
     1741 ctaatttgat gccattaatt gccgtattat tttatgatgt ttcttttttt gctttgtttt
     1801 atttgtattg gtgggaaacc gtaattattt ccgttttcca atttatcaaa atgggcaagg
     1861 cgcaaaaatt atcggagcct gatccgaatt tttcgataaa cgataaaacg ttatcttatg
     1921 agcaggtgaa cagcaaacga tatatgcgga caagttatgt cgtaattcgg tttttaatgc
     1981 tggtatttta tttattcttt atcgtaattt ttgtaggggt gctttctacc atgcaggaaa
     2041 atccaaccgc ctttgtgcgc gctttattat ttacagagcc ttgggtgaaa gttagttttg
     2101 caggattttt tgtcacgcat ttggttgaat atattttctg gcgaagtaat aaagaatatg
     2161 aacaaactag tttgcgtgaa ttaggaaccg tgttcgacac gcgtgttatg gttattcata
     2221 ttgtaattgt tctgggcact tttgctgcca tgtttgcagg tgataaatta atgcccggcg
     2281 cacaaaatgc cggcagcatt gcctatgtgt gtttatttgt tttgatgaaa atagcgatgg
     2341 atgtttatgc gcataataaa agcacgcgca gattggaagt aattaatgct ctcaccaata
     2401 gtttaataag taaaaagcca taacaaaggt aaacgagtta cgttgccgat aaatcagaac
     2461 aggtgttttt cgcaatatag ttagctgatg aattgataat tttttaataa atcaagttgc
     2521 aaaattattc cgcaaccaat tgatataccc cattccagtt tctgccctcc tcatcgtaat
     2581 ctaagccata gcctacaaca aatacatccg gaatttcgaa gcccacataa tcgcctttaa
     2641 tgaggttggg tcgcagaatt tgtttttgta aaagtgttgc tacagttatg ctggcgggtt
     2701 ttaatttttc ggcttctttt gtaagatgaa atactgtttt ccctgtatca ataatatctt
     2761 ccaaaaaaat tacatgtcgg tctttaatag gtatatcgaa accggtaaag gtttcaattt
     2821 cacctgaaac agttccggta tatgatttta tgcgcgtaaa acgaacaaca cattttccct
     2881 gaaattgtcg aattaattct gaactaaaag caaaggcgcc ggtaagcaca ataataaaca
     2941 ccggattttt atcgtgaaaa tcggctgtaa tttctttacc catttcagca atacgctttt
     3001 ggatggtatt agcatctaaa tacagcttaa atgttttgtt tcgtatttgc aaaataaatt
     3061 atttgtaacc aactatcaac tgttggccga ttttaatatt ggtatcggta atattattcc
     3121 acgattgtat ttgcgcaaca gtaacctgat attttttaga aatggcataa agtgtttcct
     3181 gtgcagctac aatatggtat ataggtaatg ctacaggtgc ttctttttta ggttcatcag
     3241 gtgtataaat atcttgttct ttttctgctt tagggtcttt tacttcagga gcaataattt
     3301 cttcttcgta gtcgataaat acgtcgttat tgtcatcctt aaattccggt tctttttcct
     3361 gtttcggcgc aggctgtttc tcttcctgct tttgttgttt tacttcaaca ggtttttttg
     3421 taggggtttt accggcaagt tttaaatcat tatgcctttt cgaacgtaga ttaattgatt
     3481 caccaaccgc aggttcctga ccttcagata ttttattgta tttataaagc tgtgataaac
     3541 gtacaccttc atcgcgagaa atactccaca tgttttctcc ttcttttaca atatgatatt
     3601 tggtatctcc ttttttgcgt ttaggttgta aataaaattt tgtacccggt gttaattcac
     3661 ctccgttttc aatatcgttg taatctttta gcaaatgtat tttgatatga tgttttttag
     3721 caagcgattc aggcgagtca ccggcttcaa cagtaatagt tggaattcga ttaaaataat
     3781 atacttcttc attataacct ccccaactta ctttcttagg ttgcttattt tcaacaggtt
     3841 tatcttcttt aacaattggg tcattttgtt ttttatctgt ttttttatct tgctttttaa
     3901 tatcggcaag tgtcatttta tccaattcat ctaaattgta ttcttcaatt agattaatta
     3961 ataaatcggc atattttggg tttgttgcat aaccggcagc ttttaaacct tttgcccaac
     4021 ctttatagtc ggtaatactt aatgtaaata attcagcata ccgggtttta tttactaaaa
     4081 atgtgctgtg gtcgcgatag gattcttcca catgatcgta tttccgaaaa cattcatcct
     4141 tagcatcatc gtcataatac actttgtcac ctttccagtc ggtgtgacat ttaataccaa
     4201 aatggttatt tgattttagt gcaagttcac tctggccggc gccactttct aaaattccct
     4261 gagccatagt aatactggca ggaattttat attcaatcat ttcccgtaca gcaatttctt
     4321 tgtataaatc gatatagtcc agcacctttt ggttaggttg ccctgccaaa accgatgcgg
     4381 ataataaaaa caatacaata gcaatgccgg tatgttttaa cccggattca attaagccct
     4441 tcatgtcaat ttcaaaatta ccctacctaa cgtcctgttt atcagcgata ttatccacaa
     4501 ataatgttat ctatccattt tttcgggcaa tagttatacc atcgcgaata gacactatca
     4561 cttgctcaac cctgggatcc aaatgaacct tctcattaaa tgcgtgaatt gcggctgtat
     4621 ctttatcgtg tttagcatcg gtaacatgcc cactccaaag cacattatcc gccaaaatcc
     4681 agcctccggg tgctacttta tcaattacta aatcaaaata attggcataa ttcgttttat
     4741 cggcatcaat aaataccaaa tcaaattgtt gggtaagggt tggaataata gtcatagcat
     4801 tgcctgttct gatagctatt ttatcctcca tattttcagc ggcaacatac tttttcacca
     4861 ttggtgtaag ctcttcatta atatctatgg taattaattg accgttttga cgtagtcctt
     4921 ttgccaaaca aattgcacta tatcctgtaa atgtgcctat ttcgagaata tgcgccggtt
     4981 taatcatgca ggatataaat tcaagaaatt tgccttgcac atggccgctc aacattcttg
     5041 gcatcattgc ttttaaatgc gtttcacggt tgagtgccga tagatgcgta tcttcggctg
     5101 tggtatattt atcgatatat gcacttaatg catcgctaat taaatcatcc ataaaaaggt
     5161 tttactttga aattacaaaa cacacaaatt tgataaataa ttttacaaat taactaaatg
     5221 ccgcgtatga agttttattt tacattaata atagtgttgt tgggaataaa tgaatcagct
     5281 tttgctcaat ataatattgc atatgccgga aaggtagatt atgctgacac tattcagtta
     5341 agtggttgct ggggatatac tgcagatgat ggtaaagaat acgcattagt gggcacaaca
     5401 catggcactt caattgtaga cgtaagtaat cctttgctgc ctgaagaatt atttttcgta
     5461 cccggatctt taagcacatg gcgcgaagta aaaacatgga acaaacatgc ttatgttact
     5521 accgaaggtg gtggtggatt gatgattatc gatttacaaa atttacctga tgcaattgat
     5581 accattagtt atcacggcac tgttgatcat ccatttaaaa ctgctcatac gctgattttt
     5641 gatgaatttg gatttggtta tttatttggt tttaatattc taaccggagc caacgaagga
     5701 gcatatatta ttgatgtaaa cgttgaccca aaacatcccg aatttgttgc tgaatttact
     5761 gatgtttatg tacacgatgg gttcgttcgt gataatcaat tatgggcagc agctatttat
     5821 gaaggtggag tttatgtcta tgatgtaaca aataaaaacg attttacatt gttaggtgtg
     5881 cagcctacct ccgcaagtgc aacacataat tgctggttga gtgatgatgg taattattta
     5941 tttactaccg atgaaatctg gaccggattt attacttcgt ttgatgtttc agatgttacc
     6001 gatataaaaa cattagacca aattaaacat ggtgctgcag attccacagt tgcacataat
     6061 acgtattatt taaatggtta tttagtaact gcacattata ccgaaggtgt tactattcac
     6121 gatgcacaac gcccgggaaa tttgatagaa gttggacatt acgacacctc accgtttggt
     6181 ccggaagtat tgctggaagg ggcttggggc gtgtatccgt attttccttc cgggactatc
     6241 attatttcag acataggtga aggattaatt gttttgcaac ctacttatac agaagcagca
     6301 tttttagaag gtaccgtaaa agatgaagta tcgttcggca atattataca tgcactaatt
     6361 gaaatttcag gaacacctgt tgttgatagc accgatattt tgggggtttt tgaaacagga
     6421 tatcatgaag cgggcattta tgatatcact atttcaaaat ctggatgtgt tactaaaaca
     6481 attaccgatg tagcattaat taacggtgaa gtaacaaatt tagatatcac attagattgt
     6541 ggtacattgt caattggtga attgccggaa aataaaataa actgttatta tttaccggat
     6601 aatggaatta tttatgtgaa taaccaaagt gacttgtcta atcatgttaa tggaagatta
     6661 attaatagtg aaggaaaaat aattcaggaa atttcattgg ctcagtctgc aacattacaa
     6721 cttgatgcaa atgcgttgcc aaaaggcttg tattatttcg aattttatac tgctgaaacc
     6781 acttttacca aaaaactgat gctttattaa aacatctggt acatcagcag ggataaatta
     6841 ggctattcaa tttaccaaga ataacgtttt taggctgatt tgcttgcggt tgaacctttc
     6901 atcttccaat ctttttcgca atctttgtcc ccgaatgcgt agcctcttat tttgttgttt
     6961 tgtggtatca tccatcctgg taaaagctca gattttaccg gcgtatggcg attcgcgaac
     7021 ggcaactacc ggatggcagt ttttaaaaat tgcacctgac gggcgttctg cagctatgag
     7081 cgaaagtttt ttggccgttg tggatgatgt gtcttcactg tattggaacc cggcgggact
     7141 tacccaactc gataccaata aatttaattt tgctgtcgat tatactgatt ttgcagccgg
     7201 aaccagtttg aattttgcag gaattaccta tcgtataaac gacaatactt tggttggtgt
     7261 tagcatgcaa tattttgacg caggggaaat ggatgtaact accgaatttt taccttttgg
     7321 aacagggcaa acatttcgtg ctaccgattt aggtttaggt gtttcacttg ctaaagaatt
     7381 aaccgaccag tttagttttg gcgtaacagc aaaatatgtt cgcgaagatt tagcatcagt
     7441 gcataatcag aatgtagttt ttgattttgg atttcaatat gatatcggca ttcaaaatac
     7501 acgatttgct gtagcgatat ctaatttcgg atttaataca caaccgggtg gcgaaatagt
     7561 tgtacttaat ttaagtaacg attcaacagt gaatgaattt acagaaattg ctgtgccaac
     7621 agttttcaga cttggttttg catgggatgc tatcaaaaat gataaacata ttttaactac
     7681 cgcattacaa ttaaatcatc caaccgataa taatgaaact tatagtatag gtgttgaata
     7741 tggatggaag catatatttt atgcccgcac aggatatacc tttgccacag attcaggcgc
     7801 ttttcctgca ttcggatttg gcacatggat taatcgtaga tttggtcaaa ttaaattgga
     7861 ttacggattt aattatttga caaatcttgg aatgataaat aaaattggta ttgcttatac
     7921 tttacccaat tcatttaaac aaagtaacga acgccaataa atgaaaaaat tatatctcgg
     7981 tattaccact gtttttgtta tgctgatttt atcgggatgc gattttttat ttggcactag
     8041 ggaagatagt accgttgatg aaatttttga agaaggtgct attgaccccg atattattca
     8101 gaatgaagca ggttatgttc ccattcttcc attttggaat gaatttgtta atcccaccga
     8161 tattttttgc ggttatgatg aaatgattta tgttgttgat gatgaaggat taaaagtatt
     8221 ggaccaaacc ggaactgttt ataatacttt ttacatacaa ggtgctacag atgtaacgca
     8281 agatcgcaga ttacatactt atgtttgtgg acgcgttgat attgatgtag ataatgatgg
     8341 cactacggaa aatctggctg cggtttatca cctgaccggt acatcttccg gtgctataca
     8401 aattgtggat acgcttattc atcctttttg tgatgttagt agaaatgtaa cttcattccg
     8461 cggcgcagaa gatgaagcag ttgaatttac cggtgtaact acacttgcag ataatacggt
     8521 ttatgtcagc aggaagggac ccaataatga tattgcagga atagcccgca ccgataatgc
     8581 agttttattt tataatgcag atggtactaa tattggctac gcaaacggcc tttcaccaac
     8641 caccagtaat ttacgttcag tttgggatat taccggaata gccacctttg ctgctcctcc
     8701 acaaagttta tccggaattt caagttcgcc ggaatttatt gttacactca ataatcctgc
     8761 ggcacaatat aaatgtttat ggatagcaca atttagcgac cctgaaagtg gaattaccta
     8821 cggcgaaaat gctgcactta ccgtttttga ttataccaaa gctgatcaat ttttatacga
     8881 acctaatcgt tttattaatc ctgcagatgt ttatatagct cccgatttta ccggttatat
     8941 ttttgttgta gatgaaggaa cagattcttt atatcagttt accagaaaag gatacgaagg
     9001 cgtaaatcca ccagcaggtt catctacaac caaacaaata attgcatcat ttggcggcga
     9061 aggaagtggg ccgtttaatt ttattaaccc aagtggcgtt gcttacctgc gtgaaacagt
     9121 ttatgttgtt gatcgcggta ataaccgaat ttgcaggtat aaattaagta cggatttaga
     9181 ataattttat ttcattgcag caacagtatt tttaacacct tcggcaggtg aagtcgcttt
     9241 tataccaaat cgtttttcga atttactgct gtctaaaata taatcctgtt catactgata
     9301 attcatttca ggaaattctt tcataattgg cataaacaga cctaataatt tcatcatcca
     9361 aaccggtaat atcatcacat caggttttac atgcatttca tttgcaaata actcaatcca
     9421 ttgtttgcct gtcagttttt ctgaagtagt tggaacatgc caggtttgat tatacgcgtc
     9481 ttcagtattt cctaaaatgg ctgtagcctt tgctgcatca ggagtatacg tgaatgtatg
     9541 aatgtaattt acaccgccaa aataattagc ttttttacct tttttgaaat tttcagcaac
     9601 tgttgtcacc aacatactct tatcatttcc cgggccataa aaatctgccg aacgcgcaat
     9661 gattgcgttt atacgattgt gttttacagc atcccaaatc atatccacta attgcttgcg
     9721 cacaattcct tttttacttg gaggatctaa aggcgcatct tctgtcatat gcggaacggc
     9781 actttttgca taactataaa cattatcaaa aaataccaac ttacaattgt gtcgttcaca
     9841 tgaatcaatt acatggcgca ttagcgttgg ccagttttgt tgccaaactt taagggaata
     9901 ttcaaatcct actactacat aaactacttc actcccggca attgcggtat caatttgttt
     9961 tgggtcgctt aaatcagcag gatgtaaagt atccgttgca tttacttttt taggattgcg
    10021 acttactaat cgaatgtctt ttgtgtatga caataattct tttgctaaat cttttccgat
    10081 tgttcctcct gagcctaaaa tagtttgcat attacttttt tttaattgac gtaaaggtaa
    10141 cccaaatatt gtatgagggg aaaatggata gcaagttaca cacttattgt aaaaaataca
    10201 aaaagtatat cccgggcatc aatctatctg ctgatttttg tccaccttaa gtgggtttag
    10261 agatggcagc tacacaaacc tgacatttgc acatagatac atttgcacat gtgcagctta
    10321 ttactatttt tacaatacat taaccaaaac attgctttac acccacatgc aattaaccac
    10381 tgaacccggc tcgttttcag caatgccctt taaacttaac gagtcaatac cgctatctca
    10441 tcttaaagta tgggtattag cgccccatct ggaaaccaat gatccgaatc tggattatta
    10501 ctacgatttt acacaaagta ttgctgagta tactaagacc tttgaagaaa ttggggtcaa
    10561 ctggatttgg cagcccgtta ctatgcagtc gtatgtaacg gttatcgaac aaattgttac
    10621 tgaacgaaat gcaggaatta actttccaat tgtattcaat atatgtgatg gtgatgaagt
    10681 gaatggcacc ccgggtgttt cagttgtgaa tttattggaa gcaaaaggac ttatttatac
    10741 gggtgcagat gcttacttct ataatatcac tacatctaaa ataaccatga aagaggcatt
    10801 cgatttagca ggagtgccaa cagcaaaatg ggaagctatt acaaacccca atcaggacat
    10861 caccggaatt tttgaacgat taggcgcacc acttattgtt aaacctgccg tatcgggtgg
    10921 ttcaatgggc gtttgtatta aaaatgtagt gaacaatgaa gctgaactaa aggagcaaat
    10981 caccaaaatg tttgatggtt atagaggatg gcatttgtcg agtggaggag tagtggtgga
    11041 agaatttatt aatggtccgg aatttacggc aatgatagtt ggctcatctg accaacaaaa
    11101 aaacatgcaa gtgtatgagc cggttgaacg catttttcat gaggctttac ctgataatga
    11161 aaagttttta tccttcgacc gtttatggga aatttatgaa gatgaaacac caatgccgga
    11221 caatggtaat ttttatcaat acgctgcagc acccgctgcg ttaaatagaa aaattaaaaa
    11281 aattagttgg gatgcatatg tatccaatgg cggcaaaggt tatacccgtg tagatttacg
    11341 tatggataa
//
LOCUS       DAORKW010000053        11225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_116_length_11225_cov_5.056580, whole genome shotgun sequence.
ACCESSION   DAORKW010000053 DAORKW010000000
VERSION     DAORKW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116_length_11225_cov_5.056580"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLB46_03760"
     CDS             <1..596
                     /locus_tag="PLB46_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRF75663.1"
                     /translation="LQIIGKEDGTNRVAGMHIVLTRDQGPIFFADTTVSFNPSSEELV
                     EIAALTAKAVRQFNIQPKVAMLSYSNFGSVENEETIRVRNAVSMLKERFPGMIVDGEM
                     QVNIAFDNDLMKENYPFSAVVDKNVNTLVFPNLHAANIGYQLMHSLGRCEVIGPILLG
                     MKRSVHILQLGSSVREIVNMVAIAVTDAQVKAKRKVG"
     gene            1284..1871
                     /gene="ruvA"
                     /locus_tag="PLB46_03765"
     CDS             1284..1871
                     /gene="ruvA"
                     /locus_tag="PLB46_03765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRF75664.1"
                     /translation="MIAYLNGEFKVKNPAYFIVECNGVGYQVNISLNTFSKLQTLDKG
                     KIHTYLHVREDAQILYGFADESEKNLFISLISVQGVGTNTARMILSSMSPGDLEEAIR
                     TENDVLIQRIKGVGPKTAKRLVIDLKDKIGKGAGATENSFGFGHNTMKEEALSALAML
                     GFTRQQGEKAIGSAMKLNPTPTNVESLIKLALKNL"
     gene            1882..9183
                     /gene="sprA"
                     /locus_tag="PLB46_03770"
     CDS             1882..9183
                     /gene="sprA"
                     /locus_tag="PLB46_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HRF75665.1"
                     /translation="MAVSKFFPQIIIGTTIGAAALAGFLYSEPEVSQHVNSSESYYLN
                     PVAPLDTPPTLPYPIDPNDGSDPFYDDENNFDLEPEVEPTIIYDPETDEYIFQPDLGT
                     EFYESPQYITFEEYIEAQGDKAIDDYWQERSDASNILGGSGQGIELTPFNKECRLFDG
                     CNVEIRPQGNVELTFGGSFQNIQNPILTENQRKQGGFDFNMDINANVLGSIGNNLKLN
                     FNYNTQATFDFENQIKLEYKGKDDQIVQGIEAGNVSLPLTTQLIPGTQSLFGIKTEFQ
                     FGRLTMTNILSQQKSQTQSIQIEGGAQKREFEIFADEYDENRHFFMSHYFRAGYEGWL
                     KTLPYVSSPINVTKMEVWVTNTTGETQNTRDIVAFADLGEPDSIYSGVIDPTGTISSL
                     TPGGLPSNYANDLYQKLSANPSTRDLNNVISTLSGAAFNMEAVQDYEKARMRKLDATD
                     YIYNPQLGFISLNTTLQPDDVLAVSYEYTYQGQTYQVGEFSSDLPPGVDTSNVLFMKM
                     LKSTSAITQIPLWDLMMKNVYSLGAFQVSSDGFRLDVLYQDPGGGLKRFIPANNANVN
                     SKPLIKLLGLDRLNNNNDPQSDGIFDFVNGTTINTQNGRVYFPVLEPFGDYLRNDAVF
                     GDDLEAEKFAYDELYNETKTIALQFPQYDRYVISGEYKSSVSSEIYLGAFNLPKGSVQ
                     VTAGGQLLTENVDYTIDYSLGRLKIINEAILNSGQQINVTFENNAFYGFQTKSLIGTR
                     LDYWINDNFTLGATWLHLSERPYTQKVNIGDDPISNSIYGFDINYTDDAPWMTRALDK
                     LPFYGTKEASTISFTGEFARFQPGHSKAIGKDDAGTIYIDDFEGSRSAIDLKFPFASW
                     VLASAPQNATDEDGDVLFPEATLFDSLEYGKNRAKIAWYNIDPLFNEDNNPSKPDHLT
                     AEDLSNHYTIQIDEKEIFPQADFETTVLTQLSTFDLAYYPDERGPYNYDVIAGPLSAG
                     LTLDGSGKLDRPETRWGGIMRNLETNDFEAANIEFVEFWVMDPFDNRGPIDGEQVVDG
                     GYLYVNLGNVSEDILKDSRMFFENGLPKDGSDDGLDQTSWGNVPRTLPITTAFDNDVE
                     SRANQDKGYDGLSSVEEADFFDTYLTYVAGVVTGTPLNLFENDPSADDYHYYRGGDYD
                     DQGLSILERYKKYNNPQGNSPTTESSPEDYPTAATTIPETEDLNDDFTLTETESYFQY
                     RIKMEPGMEASNPFITDVVVAPHSFKDGSTDSIKWYQFKIPIDQYDTRVGSIQDFKSI
                     RFIRMYMTDFEQPVVMRFARLELVRNQWRRFQFSLLNPGEYLPDDDGNETDFNVSSVS
                     IEENSARQPIPYALPPGVEREQTLGSGSSVSTYQQNEQSLSMQVCPLQDGDARAVFKS
                     LNIDLRRYGRMIMNVHAEPLPDAPLATLNDGDLHLFIRLGSDFTNNYYEYDIPLTVTK
                     LPLPAGTTEDDAVREAIWATLIDFPLDSLVAVKTLRNKDEISPLTPYSVDAGDGITYS
                     IIGNPDLGYVEEIMIGIRNPKEVAGSGQEYCAEVWVNELRLTGIDESGGWAALARADF
                     KLADLGTFTVSGNMHTAGFGTLEQQIDERYKDNFYQYAASLSLELGKFLPQDVNLRLP
                     LYASISESFSNPEFDPYELDVDLGDKLEEISTTYGTDSADTYKRSVIDYIAVKSINLS
                     NIRFERGDKGGNPHLWDKENFDLTLAYSQEYNSNPTLEYDVVDRYKAALGYSFNQKAK
                     YITPFEKVIDKKYKWLTPIRDFNFNLLPTGYSFRTELNRQFGETLMRDIYGDGLIDPT
                     FDKYFTWDRFYGLKLEPAKSIKIDFSATNNARIDEPYGKLDSQEKRDSVWMSFMDLGR
                     TTNYRHTLSATYTLPISKLPIFSWVTVKANYNTSYAWIAGSLGLADTLGNTINNTQSR
                     SINGEFNFRNLYNKSKFLKQYNSNTSPTKTNNKGSKGGDEEEEDDDKAAAVDPKKDKT
                     PTVSAPVETMLRLLISLKRVTINYTQNYGTTLPGFMQNSEIIGLNTGTMAPGFDFIFG
                     YQPTYKWLDSVSGLGYLSTATTLNYLFLQTYSEVLDVRANFEPLKDFRLDVNLNKNYS
                     KNHSEYFKNTLSVDDPLFEHLNAVDAGSFSISYSIMGTVFEKLDPKEVSATFKQFEAN
                     REIISNRWQTELNTVYSEGQFFNPLDSTLLPNYAEGYGPYSQDVLIPAFIAAYTGKSA
                     ADISLNAFSQIPKPNYRITYNGLSKLPGFKKLFSTFTVTSAYTSTLSLNSFTTDLDFD
                     GNYYAYAHVVDTLTGNFVTLYDIPNIIINEALSPLIGFDVTWINGITSKFDFKKSRTL
                     TMSFIDYQLTQIHSTEFTIGAGYKMSGFTLPIKIKGRRPNLENDLTFKFDFSFRDDIT
                     TNFRLDQDLNEPTGGMKSITLSPTIDYVVNDRFNVQLYFDRQKTVPKISTSYPITSTN
                     AGIKVRFSLAE"
     gene            9330..>11225
                     /locus_tag="PLB46_03775"
     CDS             9330..>11225
                     /locus_tag="PLB46_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRF75666.1"
                     /translation="MANSTDEKKLYLLDAFALIFRSYFAFAKNPIINSKGQNTSAILG
                     FVNTLNLLLKKTNSTHIAVCFDSAEQTQREEVFAEYKANRAETPEDIKFAVPYIKAIV
                     KAFNIPLIEKPGYEADDIIGTLAKKAKAAGYDVYMVTMDKDYGQLVEDGIYMYRPSFT
                     GNGFDTVSLEDILKKWEIENPLQVIDILGLMGDAVDNIPGVPGVGEKTAKKLIQEYGS
                     VEGLYENTDKLKGKLQENVVNNKEQALMSKHLATIMLDVPVEFEEDKLIIEDPDREAL
                     SALFQELEFRKLGKEILGEEYSVNTAKKNEQLDLFGNPVEGGNKTAVQETIDEVVAGR
                     NIDNVEHTYTLVDTPELRKKLIADLSKQKIFCFDTETTGIDANTAELVGLAFSWKTNE
                     GYYVPLPADQKEVRQIVHEFKHILEDTTKTKVAQNLKYDYLALKWYDINVRGPFEDSM
                     IAHYLLEPELRHGMDFLAETYLGYSPVSIETLIGKKGKNQLSMRDADVKKVCEYSSED
                     ADITLQLHNHFKPLLKTEELDALYNEVEIPLIEVLADMEFEGINLDVPFLADYSKVLE
                     KDIIELEKKIHEIAGVRFNIDSPKQLGEVLFTKLKIPYEGQKTKTGQLSTGEDILAKL
                     AHNHPIAN"
BASE COUNT         3706 a         1973 c         2217 g         3329 t
ORIGIN      
        1 ctcttcaaat tataggaaaa gaagatggca ccaatcgcgt tgccggtatg catattgtgc
       61 ttacccgcga ccagggacca atattttttg ccgacaccac cgtgagcttc aatccaagca
      121 gcgaagaact ggtggaaatt gctgcgctga ctgccaaagc tgtccgccaa tttaacattc
      181 agccaaaagt tgccatgtta tcttattcca atttcggctc agttgaaaat gaagaaacca
      241 ttcgtgttcg aaacgcagtg agtatgttga aagagcggtt tccgggaatg attgtagatg
      301 gtgaaatgca ggtgaacatt gcttttgaca acgatttaat gaaagaaaat taccccttca
      361 gtgcggtagt agataaaaac gtaaatactt tggtattccc taaccttcat gctgccaata
      421 tcggctatca gctgatgcat tcgttgggta gatgtgaagt tattggacca attttattgg
      481 gtatgaaacg ttctgtacat attttacaac taggttcatc cgttcgcgaa attgtgaaca
      541 tggtggcaat tgcagtaaca gatgcgcagg tgaaagcgaa gaggaaggtt ggttaatgtg
      601 ctgatgggtt aatgtgttga tgtgctgatt aatgtgttga tgtaccaatg taccgatgta
      661 ccaattaaca gccaatgatg tggcgctgta tccgccgggg cggaaacggc agggttggag
      721 gtggaatgac attcagagtt ccgtggtgat aaaccaaatt ggttggatga attaccattc
      781 ggggcaaccg tagccgtcaa cccacgtgtt gaccaatggt tatcgcgatt gtttatgtgc
      841 tgatttgata atgtgttgat gtgctgatta atgtgcgaat gtaccaatgt accgatgtac
      901 caattaacag ccaatgatgt ggcgctgtat ccgccggggc ggaaacagcc gggttggagg
      961 tggaatgtaa ttcggagttc tgtgacaaaa aaccaattga ttggacaaaa taccatccaa
     1021 ggcaaccgta gccgtcaacc cacgtgttga catctgtttg tcgcgaatga taatgtgtca
     1081 accgcgttat tggtaaaata acatccaaac caaccatccg ccgtagcgga gggttgaatt
     1141 ttaaattaaa agaaaattgt gaggtttatt aggcgtttca aacccttaaa acctccggaa
     1201 aaatataatt ttaacacaaa aaaaccggag attgcatcgc aatcccccat ttgctaattt
     1261 gctaattagc taattaacta attatgatag catacttaaa cggtgaattc aaagtgaaaa
     1321 acccagccta ttttattgtg gagtgcaatg gggttggcta tcaggtaaat atcagtttaa
     1381 ataccttttc taagcttcaa actttggata aagggaaaat acacacatat ttgcatgttc
     1441 gtgaggatgc gcagattttg tacggatttg cggatgaaag tgagaagaat ctgtttatca
     1501 gtttaatctc cgttcaggga gttggaacca acacggcaag aatgattttg tcgagtatga
     1561 gtcccggcga ccttgaagaa gcaattcgca ccgaaaacga tgtattaatt cagcgcataa
     1621 aaggggttgg tcccaaaacc gcaaaacggc tggtaatcga ccttaaggac aagattggca
     1681 aaggagccgg cgcaactgaa aattcttttg ggtttggaca caatactatg aaagaagaag
     1741 cgttaagtgc tctggcgatg cttgggttta cacgtcaaca aggcgaaaag gctattggaa
     1801 gtgcgatgaa actgaaccct acacccacga atgtagaaag cttgattaaa ttagcattga
     1861 agaacttgta aacaagaggt tttggcagta tcgaaatttt ttccccaaat aataattgga
     1921 actactattg gtgccgcggc actggccggt tttttatatt ccgaaccgga ggtttctcag
     1981 catgttaatt catctgaatc ctattaccta aatccggttg ccccactcga cacccctcca
     2041 acattaccct atcctatcga ccctaacgat ggctcggacc cattttatga tgacgaaaat
     2101 aacttcgacc tggagccgga agttgaaccg accattattt acgacccgga aacggatgaa
     2161 tatattttcc aaccggacct tgggaccgaa ttttatgaaa gtccgcaata catcacgttt
     2221 gaagaataca ttgaagctca gggtgacaaa gcaattgatg attattggca agaacgttcc
     2281 gacgcttcta acattttagg tggtagtggt cagggaattg aactcacccc tttcaacaaa
     2341 gaatgtcgtt tgtttgatgg ttgtaacgtt gaaattcgcc ctcagggtaa cgttgaactc
     2401 acttttggtg gaagttttca gaatattcaa aatcccatcc ttactgaaaa tcagcgtaaa
     2461 caaggtgggt ttgattttaa tatggatatt aatgctaacg tattagggtc tattggtaat
     2521 aatctaaaac tgaattttaa ttacaatacg caggcaacat tcgatttcga aaatcaaatt
     2581 aaactagaat acaaaggcaa agatgatcag atagtgcaag gtattgaagc cggtaacgtg
     2641 agtttgcctt taaccactca gttaattccc ggaacacaaa gtttatttgg tattaaaacg
     2701 gaatttcaat ttggccgttt aacgatgacc aatattcttt ctcaacaaaa atctcaaaca
     2761 caaagtatac aaattgaagg tggtgcgcaa aaacgcgagt tcgaaatatt tgcagatgaa
     2821 tatgatgaaa atcgtcactt ctttatgtcg cattatttcc gcgcaggtta cgaaggctgg
     2881 ttaaaaactt taccttatgt aagctctcca attaatgtta ccaaaatgga agtttgggta
     2941 acgaatacta caggtgaaac acaaaataca cgcgacattg tggcatttgc cgatttaggt
     3001 gaaccggatt caatttattc gggtgtaatt gatcctacag gaacaattag cagtttaaca
     3061 cctggtggat taccttccaa ctatgcgaat gatttatatc agaaattaag tgctaatcca
     3121 agcacacgcg atttaaataa tgttatctct actttatccg gcgcagcatt taatatggaa
     3181 gctgtgcaag attatgagaa agcaagaatg cgcaaattag atgcaactga ttatatctat
     3241 aatccacaat taggttttat ttcattaaac accacattac aacctgatga tgttttagcg
     3301 gtatcatatg aatatactta tcagggacaa acatatcagg tgggtgaatt ttcgagtgac
     3361 cttcctcctg gtgtagatac atcgaatgta ttattcatga aaatgctgaa aagcacttct
     3421 gcaattacac aaattccgtt gtgggatttg atgatgaaaa acgtgtattc acttggtgca
     3481 ttccaggtga gtagcgatgg gttccgttta gatgttttat atcaagatcc cggtggtgga
     3541 ttaaaaagat ttattcctgc taataatgcg aatgttaaca gcaaaccatt aattaaatta
     3601 ttaggtctcg accgtttaaa taataataat gacccgcaaa gtgatggtat ttttgacttt
     3661 gtaaatggaa caaccattaa tacacaaaat ggtcgtgtat atttcccggt actcgaacca
     3721 tttggtgatt atttacgcaa cgatgccgtt tttggggacg acttagaagc tgaaaaattt
     3781 gcgtatgatg aattgtataa cgaaacaaaa acaattgccc ttcaatttcc gcaatacgac
     3841 agatatgtaa tttccggtga atataaatcc agtgtttctt ctgaaattta tttaggtgca
     3901 tttaatttac ctaaaggttc ggttcaggta actgcaggcg gacaattact gactgaaaat
     3961 gtggattaca ccatcgatta ttcattgggt agattaaaaa ttattaatga agcgattctg
     4021 aattccggtc agcaaataaa tgtaacattt gagaataatg cattttatgg cttccagaca
     4081 aaatcattaa ttggaacaag attagattat tggattaatg ataatttcac tttaggtgct
     4141 acatggttgc atttaagtga acgtccttac acccaaaaag taaatattgg tgacgacccg
     4201 atttctaaca gcatttatgg ttttgatatc aattataccg atgatgcacc atggatgaca
     4261 agagcgttag ataaattacc attttacggc actaaagaag catctaccat ttcatttacc
     4321 ggtgaatttg cacgtttcca accgggacac agcaaagcaa ttggtaaaga tgatgccggt
     4381 acaatttata tcgacgactt tgaaggaagc agaagtgcta tcgatttgaa atttccattt
     4441 gcaagttggg tattagcgag tgcgcctcaa aatgcaactg atgaagatgg tgatgtatta
     4501 tttcctgaag caactttatt cgatagtctt gaatacggaa aaaatcgtgc gaaaattgcg
     4561 tggtataata tcgacccatt atttaatgaa gataataatc catccaagcc cgaccattta
     4621 actgctgaag atttatccaa tcactatacc atacaaattg acgaaaaaga aattttccct
     4681 caagcagatt ttgaaactac tgttttaacg caattatcaa ctttcgactt agcttattat
     4741 ccagatgaac gtggtcctta caattatgat gtaattgccg gccctttgtc tgcaggttta
     4801 actttagatg gtagtggtaa attagaccga cctgaaacgc gttggggtgg tattatgcgc
     4861 aacctcgaaa ccaacgactt tgaagcagcc aatatcgaat ttgtcgaatt ttgggtaatg
     4921 gacccatttg ataatcgcgg tccaattgat ggcgaacaag ttgttgatgg tggatattta
     4981 tatgtaaact taggtaacgt ttcagaagac atattaaaag attcaaggat gttttttgaa
     5041 aatggtttac caaaagatgg cagtgatgat ggattagacc aaacgagttg gggaaatgtt
     5101 ccaagaactt taccaattac tacagcattt gataatgacg tagaatcacg tgccaatcag
     5161 gataaaggtt atgatggttt aagcagcgtt gaagaagcag atttttttga cacttattta
     5221 acctatgttg caggtgttgt tacaggaaca ccattgaatt tatttgaaaa tgacccttct
     5281 gccgatgatt atcattatta tcgcggtggc gactatgatg atcaggggtt gagtattttg
     5341 gagcgctata aaaaatataa taaccctcag ggcaactcac caaccacaga atcatctcct
     5401 gaagattatc cgacagcagc aactactatt cctgagacgg aagatttaaa tgatgatttt
     5461 acattaacag aaactgaatc gtatttccaa tatcgtatta aaatggaacc gggaatggag
     5521 gcatcaaatc catttattac agatgttgtc gttgcccctc actcatttaa agatggtagc
     5581 accgattcta ttaaatggta tcagtttaaa attccgatcg accaatatga tacacgcgta
     5641 ggaagcatcc aggattttaa atctattcgt tttatcagaa tgtatatgac agattttgaa
     5701 caacctgttg taatgcgttt tgctcgttta gaattagtac gtaatcaatg gagaagattc
     5761 cagtttagtt tattaaatcc tggtgaatat ttaccggatg atgatggcaa cgaaactgat
     5821 ttcaacgtat catctgtaag tattgaagaa aatagtgctc gtcaaccaat tccttatgca
     5881 ttacctccgg gtgttgaacg tgaacaaaca ttaggaagcg gaagttcagt ttctacttat
     5941 cagcaaaacg aacaatcgtt gagcatgcag gtttgtcctt tacaagatgg tgatgcaaga
     6001 gcagtattta aatcattgaa tattgattta cgtcgttatg gtcgtatgat tatgaacgtg
     6061 cacgcagaac cacttcctga tgcaccgttg gcaacattaa atgatggcga cttacattta
     6121 tttattcgtt taggttctga ctttaccaat aactattacg aatatgatat tccgttaacc
     6181 gtaactaaat taccattacc agcaggaacc acagaagatg atgcagttcg tgaagctatt
     6241 tgggctacgt taattgattt cccactcgac tcattagttg ccgttaaaac attacgtaat
     6301 aaagatgaaa tttcaccttt aacaccatac tctgttgatg ccggtgatgg cattacttat
     6361 tccattattg gtaatcctga tttaggatat gtggaagaaa ttatgattgg tattcgcaat
     6421 ccaaaagaag ttgctggctc aggtcaggaa tattgtgctg aagtttgggt gaatgagttg
     6481 cgattaaccg gtattgatga aagtggcgga tgggcagcat tagcaagagc cgattttaaa
     6541 ttagctgacc tcggaacatt tactgtttcg ggtaatatgc ataccgcagg atttggaaca
     6601 ttagaacaac aaattgatga acgttataaa gataattttt atcagtatgc agcttcctta
     6661 agtttagaat taggtaaatt tttacctcag gatgtaaact tacgtttacc attgtatgcg
     6721 agtatttccg aatcattcag taatcctgaa ttcgaccctt atgaattaga tgttgacctt
     6781 ggcgacaaac tggaagaaat atctactact tatggcaccg attcagctga tacttacaaa
     6841 cgttcggtaa tagattatat cgccgtaaaa agtattaact tatctaatat ccgtttcgaa
     6901 cgcggcgaca aaggtggtaa tccgcattta tgggataaag aaaattttga tttaacatta
     6961 gcttactcac aggaatataa cagtaacccg acattagaat atgatgttgt ggatcgttac
     7021 aaagcagctt taggatatag cttcaatcaa aaagcaaaat atattacacc atttgaaaaa
     7081 gtaattgata aaaaatataa atggttaaca cctattcgcg atttcaattt caatttatta
     7141 ccaacgggtt atagtttccg caccgaatta aacagacaat ttggcgaaac cttaatgcgc
     7201 gatatttatg gtgatggatt aattgaccca acatttgata aatattttac atgggataga
     7261 ttttatggtt taaaactgga acctgcaaaa tcgattaaaa ttgatttcag tgctactaac
     7321 aatgcccgta ttgatgaacc atatggtaaa ctagattcac aggaaaaacg tgattcggtt
     7381 tggatgagtt ttatggattt aggaagaact accaattacc gtcatacttt atccgcaacg
     7441 tatacattac caatcagcaa attaccaata ttcagctggg ttacagttaa agctaattac
     7501 aataccagtt atgcatggat agcaggttct cttggtttag ccgatacatt aggaaatacc
     7561 attaataaca ctcaatcgcg ttcaattaat ggtgaattta atttccgtaa tctttacaat
     7621 aaatctaaat tcttaaaaca atacaattcc aatacatcac caactaaaac aaataacaaa
     7681 ggttctaaag gtggtgacga ggaagaagaa gatgatgata aagctgcagc tgttgatcct
     7741 aaaaaagata aaacacctac agtatctgct ccggttgaaa ccatgttgcg tttattaatt
     7801 agtttgaaac gtgttaccat taattacact caaaattatg gaacaacatt gccgggcttt
     7861 atgcaaaatt cagaaatcat tggattaaac accggcacaa tggctcccgg gttcgacttc
     7921 atttttggtt atcaacctac ctataaatgg cttgatagtg tttccggttt aggttatttg
     7981 agcaccgcta caaccttaaa ctatttgttt ttacaaactt atagtgaagt gcttgatgtt
     8041 cgtgccaatt ttgaaccgtt aaaagatttc agattagatg tgaacctcaa taaaaattat
     8101 tcaaaaaatc attctgaata ttttaaaaat acattaagtg ttgacgatcc gctgttcgaa
     8161 catttaaatg cggttgatgc gggaagtttc agcatcagct atagcattat gggaaccgtt
     8221 ttcgaaaaac ttgacccgaa agaagtttct gcaacattta aacagtttga agcaaatcgt
     8281 gaaattattt ctaaccgctg gcaaacagaa ttaaatactg tttactcaga aggccaattc
     8341 tttaacccgc ttgattcaac attgttgcct aactatgctg aaggttacgg tccgtattca
     8401 caggacgtat taattcctgc attcatagct gcatataccg gaaaatcggc agcagacatt
     8461 tcgttaaatg catttagtca aatacctaaa cctaactaca gaattaccta taatggttta
     8521 agtaaattac cagggtttaa aaaactgttt tctacattta ccgtaaccag tgcttacact
     8581 tcaacactgt cgctgaacag ctttaccact gatctagact ttgatggtaa ttattatgct
     8641 tatgcccacg tggtagatac attaaccgga aactttgtaa cactttacga tattcctaat
     8701 attattatta atgaagcttt atcaccgtta attggatttg atgttacctg gataaatggt
     8761 atcacatcga aatttgattt caaaaaatca agaacattaa ccatgagttt catcgattat
     8821 cagttaacac aaattcattc aactgaattt acaattggtg ccggttacaa aatgagtggc
     8881 ttcaccttgc caatcaaaat aaaaggtcgt cgtccaaatc ttgaaaacga tttaacattt
     8941 aaattcgact tcagcttccg tgatgatatt acaaccaatt tcagattaga ccaggactta
     9001 aatgaaccta caggtggtat gaaaagtatc acattgtcgc caacaatcga ttatgtggtt
     9061 aacgacagat tcaatgttca gttgtatttc gacagacaaa aaacagttcc aaaaatttcg
     9121 accagctacc ctatcacttc taccaatgca ggtataaaag tgagatttag tttagcagaa
     9181 taagaaattg aaacattata cacctagccg gcagctttat acaagttttg ccggctattt
     9241 ttttgagatg gcatcaaaac aacccactat cacttgagcc cttattctgc tcctcaatat
     9301 gccttatatt tgcagtataa ctcaaaaaga tggcaaattc aaccgacgaa aaaaaactct
     9361 atttactaga tgcatttgca ttaattttca ggtcctattt tgcgtttgca aaaaatccga
     9421 taataaattc taaagggcaa aatacttctg ccattttagg ttttgtaaac acccttaatt
     9481 tattgctgaa aaaaaccaat tcaacacata ttgcagtttg tttcgactca gcagaacaaa
     9541 cacaacgtga ggaagttttt gctgagtata aagccaacag agcagaaaca ccggaggata
     9601 tcaaatttgc cgttccatat atcaaggcta ttgtaaaggc atttaatatt ccattaattg
     9661 aaaaaccggg ttatgaagcc gatgatataa ttggcacgct ggctaaaaaa gcgaaagccg
     9721 ccggatatga tgtttacatg gttaccatgg ataaagatta tggccaattg gtggaagatg
     9781 gtatttatat gtatcgcccg agttttactg ggaatggatt tgacaccgtt agtttggaag
     9841 atattttaaa aaaatgggaa atagaaaatc cacttcaggt aattgatata ttagggttaa
     9901 tgggtgatgc agttgataat attcccggcg ttcccggagt tggtgaaaaa actgcgaaaa
     9961 agttaattca ggaatatggt tcagtagaag gtttgtatga aaataccgat aaactaaaag
    10021 gcaaactaca agagaacgtt gtcaataaca aagagcaagc cctcatgagt aaacatttag
    10081 caaccattat gttagatgtt cctgttgagt tcgaagaaga taaattaata attgaagacc
    10141 ctgaccgcga agcactttct gcactttttc aagaattgga atttagaaag ttaggtaaag
    10201 aaatattagg tgaagaatac agcgtaaaca cagctaaaaa aaatgagcaa ctcgatttat
    10261 ttggcaaccc tgttgaaggc ggcaacaaaa ctgccgtgca ggaaactatt gacgaagttg
    10321 ttgcaggaag aaatattgat aatgtagaac atacctacac attggtagac acacctgaat
    10381 tacgaaaaaa acttatagct gatttatcta aacaaaaaat attttgcttc gatacggaaa
    10441 ctaccggtat tgatgccaac acagccgagt tggtaggatt ggctttttcg tggaaaacaa
    10501 atgaaggata ttatgtgccg cttccggcag accaaaaaga ggtgcgacaa attgtacatg
    10561 aatttaaaca cattttagaa gatacaacca aaactaaagt agcgcaaaat ttaaaatatg
    10621 attacctcgc attaaaatgg tacgacatta atgtaagagg tccttttgaa gacagtatga
    10681 ttgcgcatta tttattggaa cctgaattac gtcatggcat ggacttttta gcggaaacct
    10741 acttaggcta ctcaccggtt tctattgaaa cattaattgg caaaaaagga aaaaatcagt
    10801 tgagcatgcg cgatgcggat gttaaaaaag tttgtgagta ttcttctgaa gatgctgata
    10861 tcactttaca actgcacaat cactttaaac ccctgctcaa aacagaagag ctggatgcat
    10921 tatataatga agtagaaatt cctttaattg aagtgttggc ggacatggaa tttgaaggta
    10981 tcaatctcga cgttccgttt ttagcagatt acagtaaagt gctggaaaaa gatattattg
    11041 aacttgaaaa gaaaattcac gaaatagctg gagtaagatt taatattgat tcgccaaaac
    11101 aattaggtga agtattattt accaaattaa aaattcctta cgaaggtcaa aaaacgaaaa
    11161 ccgggcaatt atcaaccggt gaagatattc tggcaaaatt ggcacacaat catccgattg
    11221 ccaat
//
LOCUS       DAORKW010000054        10900 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_121_length_10900_cov_6.070724, whole genome shotgun sequence.
ACCESSION   DAORKW010000054 DAORKW010000000
VERSION     DAORKW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121_length_10900_cov_6.070724"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..595)
                     /locus_tag="PLB46_03780"
     CDS             complement(17..595)
                     /locus_tag="PLB46_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF75667.1"
                     /translation="MKRHLGVIIFLVYNSLHLSAQDSTTVSQNIDTTTYVYGNVMYEN
                     GNYDAATGVYEQLLAKNGPSDEIYFNLGNCYYKSNAYGKAILNYERALFFNPGNEDAQ
                     YNLELANQRIRDQIEPVNESIFTIWWNNFIQIFTAHTWTILAVIFMWLALAGFVIYRL
                     PKFVAWQRPRFFFFFFKLIFSILFFFCWFGWE"
     gene            complement(592..2385)
                     /locus_tag="PLB46_03785"
     CDS             complement(592..2385)
                     /locus_tag="PLB46_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HRF75668.1"
                     /translation="MKTWLTVIVLFIATIPIMAQNATVTATVSSNKVGLSDRFKYTIE
                     VTNSADAKDFRPPSLTDFRILGGPNQSMSYQNVNGNISQSVAYSYILQPKELGKFTIG
                     AAYVKVNGQTLTSKPITIEVIDKPATTDNAKDQKSSSSPTTDLETYLKDNVFIKTEVS
                     DDEVYKGENIAVTLKLYVNNNGSIYGPRGFQNIITPKYDGFYAEEIDLPDQQIKTEVI
                     NGVSYHVNIIKKTILTPQRSGSLEVDPLSIDAIFAVITKSGKSFNPFASNAKDVMVTI
                     KSNPVKIKVNELPPNTPDDFNGAVGKFTMKTQINATETKTDEPLTYRIIISGTGNLEL
                     FNAPELNLPPGWETYDPKTTTASGAKTFEYLLIPRSPGDFTIPSYTWSYLDPGSKKYV
                     SLASEAYNVKVEAGPGYNPATGNFATNKEEVEALASDIRFITKDNPSYYNEKPSFAGS
                     GLFYTLFMLPFVAGIGLFAFTFVNNRKQHDVVALRYNSANANAKKRLTKAAEFVASNN
                     GRSFYDETIRALWGYLSDKLAINRSELTKENIEQVLAGKNVSHATAQSLIDLLNDCEM
                     SLFAPPTGGSSLQQVYASAVDLITKLENEIK"
     gene            complement(2387..3097)
                     /locus_tag="PLB46_03790"
     CDS             complement(2387..3097)
                     /locus_tag="PLB46_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF75669.1"
                     /translation="MKKNETMRIVVLTFLLLISYTGFAQNSNAIIKEGNDLYEQKDYK
                     GAQQKYEQAVTETPDSDAGNYNLGNTYYQQQEYDKAAAQYERAAQAASDPETRANAYH
                     NLGNAYLQQKKYEESINAYKQSLRDKPTDDNTKYNLAYAQKMLKQQQQQQQQNQDKNQ
                     QKKEEPKKQDQQKQDQNQQKQEQQQKEQQQPAQPKEYSKEELERILQSLNNDDKNVQE
                     KVNKQKSQSGNADAEKDW"
     gene            complement(3084..4130)
                     /locus_tag="PLB46_03795"
     CDS             complement(3084..4130)
                     /locus_tag="PLB46_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF75670.1"
                     /translation="MEFENKIMLYGLAVIPLLMLVFYAYVAWRKKALAKMGDQHLLEQ
                     LMNRTSMKRKWIKFSLLLAACAFIILGLANLRLGSKKQKVKGESAEVMICFDVSNSML
                     AEDVKPNRLAQAKLSAAQLIEKLASNKIGLIVFAGESYVQMPLTSDSRAALMYLNNIN
                     TGTITNQGTAIGSALETAIKAFDNGGEKDNKKGKAIIIITDGESHDDNAEAMAKEAAD
                     NDIKIITLGVGTPTGAPIPVRKGNTVNGFKKDREGNVVLTKLNEKMLQQLALEADGVY
                     MNLNGGKKVVQDVYEQIDALDKTADDTYQFTEYANHFQLFLGIGLFLLTLEFFMSDKK
                     PKWLEKVNLFDEKK"
     gene            complement(4132..5142)
                     /locus_tag="PLB46_03800"
     CDS             complement(4132..5142)
                     /locus_tag="PLB46_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF75671.1"
                     /translation="MNEFISNIKFEDPWLLLLLLLVPVYIYYLRTQANNKYVAFKISN
                     TSGFKDYVPAKIKWMRLLPVLRITAVILLIIAIARPQTGFSNKKISSQGIDIMLSLDV
                     SPSMYAIDFKPNRMEAAKVAANEFIDSRPNDRIGLVVFAGEAFTQCPATLDHEMLKAQ
                     VATADNWYLKDGTAIGDGLFLAVNRLADTVNVNTKVIILLTDGVRIGGKYSPVDAANA
                     AKQLNVRVYTIGVGSESNTPIPVVDKNGRKIFELDPRISFDETMLKEVANLTGGQYFK
                     ATSKEKLSEIYQQIDQIEKQKFEVDITQRYDEHFFYFALIGFILLLIEILLTNTIFRS
                     LT"
     gene            complement(5139..6023)
                     /locus_tag="PLB46_03805"
     CDS             complement(5139..6023)
                     /locus_tag="PLB46_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75672.1"
                     /translation="MPLTAQVSVEVQTDKFDYAIGDYIQITIKATGDSTNTYYWPPVE
                     EIAPYEIISAQPIDTLTADGKTMFTQQITYSIYEEGKYYFPQVFILYKMQRDTTTLLA
                     MSDSLPFNVITPEVDTTAAFKPIKDIKLVTVRDFTWMQILLLLHAIIAIAFLVYFFFI
                     RKEDKPTPKPLAKPIRPLHEIFLEQLRALDEKKLWQQDRLKFYYSELTDILRDYLEQR
                     FYINAMESTSDEIIEQIQHIPEAKAFTNDISFILQLADMAKFAKSRPLANENVRAMEL
                     TVNFVENTKIIPPTPEKI"
     gene            complement(6070..6834)
                     /locus_tag="PLB46_03810"
     CDS             complement(6070..6834)
                     /locus_tag="PLB46_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRF75673.1"
                     /translation="MSFSEVREYTFGDDVRNIEWNVTARMSHPYIKVFEEERELTVML
                     LIDISQSSFFGTQLQMKNQLITEIAAVLSFSAINNNDKVGVILFSDRIEKFIPPKKGK
                     SHILRIISDLLELTPEGKGTNIAQALQYFNNVVNKKSIAFVFSDFMDKGYEKPLMIAA
                     RKHDVIGVHIHDKREEELPDAGIVRMFDPEKNDTILVDTSNKKVRDRYRSNYLKNVQA
                     FKSTFVKSGADTISIHSQKPYINELHSFFRKRIMMK"
     gene            complement(7008..8066)
                     /locus_tag="PLB46_03815"
     CDS             complement(7008..8066)
                     /locus_tag="PLB46_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRF75674.1"
                     /translation="MNTELFLCSENFNRPNLTNMDTAASTDIKELNERIHQESAFIDL
                     LRLETAKAIVGQKNMIDRLLLGLLTQGHILLEGVPGLAKTLAIKSLASAINAKFSRIQ
                     FTPDLLPADLIGTMIYNQKDNQFTVKKGPIFANFILADEINRAPAKVQSALLEAMQEK
                     QVTIGENTYKLEEPFLVLATQNPIEQEGTYPLPEAQVDRFMLKVVIHYPSKEDEKLII
                     RQNVTGAINKIAQVVKPEDIIRARDLVREVYMDDKIENYILDIVFATRKPEDYKLSKI
                     KHLIAYGGSPRASINLAMASKAYAFIKRRGYVIPEDVRAICQDVLRHRIGLTYEAEAE
                     NITAEDIIAEILNVVEVP"
     gene            8188..8583
                     /locus_tag="PLB46_03820"
     CDS             8188..8583
                     /locus_tag="PLB46_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRF75675.1"
                     /translation="MQNKISIQANLGNVQYRTTLSNGKHTLLADEPESEGGLATGPTP
                     DEFLAMALAACTSITLRMYSQRKNWDLGEIAVAVDMQRQDDGSAVFERKITFEKKPDA
                     ATCERLMIVADKCPVHKTLSHVNTIISSL"
     gene            8592..8963
                     /locus_tag="PLB46_03825"
     CDS             8592..8963
                     /locus_tag="PLB46_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HRF75676.1"
                     /translation="MNDIVSSNEIKMVIDTFYEKVRADEMIGFIFNDIAKVDWEHHMP
                     IMYNFWESTVFGTGTYTRNAMTPHFVLQDKVKFTAAYFERWVFLFVTTVDELYTGENA
                     EIMKMRATSIAGLMQNKLGIN"
     gene            9214..9753
                     /locus_tag="PLB46_03830"
     CDS             9214..9753
                     /locus_tag="PLB46_03830"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF75677.1"
                     /translation="MTIILTILMIIGIIISVILITALILPKKYKVLREATIQQPQQKV
                     FDYIKYIRNQDGFSVWANMDPNMKKTYKGDDGRVGFVSAWESDNKKVGAGEQEIIRVD
                     EGKGIVTTLRFFKPFPGEAVAQFMTSPAENNATKVSWQLDSAMKYPMNFMLLFMNMDK
                     MLGADMEKGLENLKNKLAE"
     gene            complement(9756..10169)
                     /locus_tag="PLB46_03835"
     CDS             complement(9756..10169)
                     /locus_tag="PLB46_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRF75678.1"
                     /translation="MRTTVCRRATFNAAHRLFNPDWDEATNDKVFGLCNNPNFHGHNY
                     TLFVKITGEINPQTGYVMDLKIVNDLIKELVIEPFDHNNLNLDVPEFAALNPTAENIA
                     KVIYDKFRAKLDAEFDVKIELYETENNFVIYPGWD"
     gene            10235..10723
                     /locus_tag="PLB46_03840"
     CDS             10235..10723
                     /locus_tag="PLB46_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HRF75679.1"
                     /translation="MVVTDSIHAFTFRSIDGGTIDFSAFKGKKVLVVNTASDCMYTDQ
                     YKQLQELHDNYADKLVIVGFPSNDFGEQEPGTDREIKNFCSYKYGVTFPLASKSEVIG
                     VNSNPIFKWLISLPFENTLNKSIVWNFQKFLLNEEGKLIAVFPPATDPINDELLAFLN
                     EN"
BASE COUNT         3265 a         2020 c         1945 g         3670 t
ORIGIN      
        1 attgtggtaa aaaaaattat tcccacccaa accagcaaaa aaaaaaaagt atagaaaaaa
       61 ttaatttaaa aaaaaaaaaa aaaaaccggg gacgttgcca agccacaaat ttcggcaagc
      121 gataaataac aaatcccgcc aatgctaacc acataaaaat tacggcaaga attgtccagg
      181 tatgtgctgt aaaaatttga ataaaattat tccaccaaat agtaaatata gattcattaa
      241 ccggctcaat ctggtcgcga atacgttgat tggctaactc caaattatat tgagcatcct
      301 cattacccgg attaaaaaac aaagcccgtt cataatttaa aatggctttg ccataagcat
      361 ttgatttata ataacaatta cccaaattga aataaatttc atctgaaggg ccatttttag
      421 cgagtagttg ttcatataca cctgttgctg catcgtagtt accattttca tacattacgt
      481 tgccatacac ataagtggta gtatcaatat tttgtgatac tgttgtactg tcctgcgctg
      541 aaagatgcag actattgtaa accagaaaaa tgattactcc taaatgtcgt ttcattttat
      601 ttcgttttcc agttttgtaa ttaaatcaac tgcagatgca tatacttgct gtaatgatga
      661 gccgccagtt ggtggtgcaa aaagcgacat ctcgcaatcg ttcaacaaat caattaatga
      721 ttgtgcagta gcatgcgaaa cattttttcc ggccagcacc tgctcaatat tttctttcgt
      781 caactcactt ctgttgattg ccagtttatc ggacaagtaa ccccaaagtg cacgaattgt
      841 ttcatcataa aatgaacgac cattattcga ggctacaaat tcagctgctt tggtaagtcg
      901 ttttttagca ttcgcatttg cactgttgta acgcaatgcc acaacatcat gttgtttacg
      961 attatttaca aatgtaaaag caaaaagccc tatccccgcc acaaaaggca gcataaataa
     1021 agtataaaat aaaccgcttc cagcaaatga tggtttttca ttataataag aaggattgtc
     1081 tttagtgatg aaacgaatat cactggctaa cgcttccact tcttctttgt ttgtcgcaaa
     1141 atttcctgtt gcaggattat atcccggacc tgcctcaact tttacattat atgcctcaga
     1201 agcaagtgaa acatattttt tagaaccggg atccaaatac gaccaggtgt acgatggaat
     1261 cgtaaaatcg cccggcgaac gcggaattaa taaatattca aaagttttag cacctgatgc
     1321 ggttgttgtt ttggggtcat atgtttccca tcccggaggc agatttaatt ccggcgcatt
     1381 aaataattcc aggttgccgg ttccggaaat aataattcta tatgtcaacg gctcatccgt
     1441 ttttgtttcg gtagcattta tttgtgtctt catggtaaac ttacctacag caccattaaa
     1501 atcatccgga gtattgggtg gtaattcgtt tactttaatt tttacgggat tagatttaat
     1561 agttaccatt acatctttag cattagatgc aaatggatta aaacttttac cacttttggt
     1621 aattaccgca aaaatggcat caatactcag cggatcaact tcaagcgagc ctgagcgctg
     1681 aggtgttaaa attgtttttt taattatgtt tacgtggtag ctaacaccgt ttattacttc
     1741 tgttttaatt tgttggtccg gcaaatcaat ttcttcggca taaaaaccat cgtatttagg
     1801 tgtaataata ttctgaaatc ctcttgggcc gtaaatactg ccattgttgt tgacatataa
     1861 ttttaaagtg accgcaatat tttccccttt atatacctca tcgtcgctga cttcagtttt
     1921 gataaaaaca ttatctttta aatatgtttc aaggtcagtt gttggtgagc ttgatgattt
     1981 ttggtctttt gcattatcag tagtagccgg cttgtcaatt acctcgatag taattggttt
     2041 agatgttagt gtttgtccgt ttacttttac gtatgcagct ccgatggtaa attttcctaa
     2101 ttcttttggt tgtaaaatat acgaatacgc aactgactga gaaatattac catttacatt
     2161 ttggtaactc atactttgat tagggccgcc caaaattcta aaatcggtaa gggaaggcgg
     2221 acgaaaatct ttggcatcgg cgctattagt tacttctata gtatatttaa atctatcgct
     2281 cagccctacc ttattgctac ttactgtagc cgtaacagta gcattttggg ccattattgg
     2341 tatagtggcg ataaataaaa caataactgt tagccaggtt ttcatattac caatcttttt
     2401 ctgcatcagc attgccggat tgtgattttt gcttattcac tttttcctga acatttttat
     2461 cgtcattatt taatgattgt aaaatgcgtt caagttcttc ttttgagtat tctttaggtt
     2521 gagcaggttg ttgttgttct ttttgctgtt gctcttgctt ttgttgattt tgatcttgtt
     2581 tctgttgatc ctgcttttta ggctcttcct ttttttgttg atttttatcc tgattttgct
     2641 gttgttgctg ctgttgttgc ttcagcattt tttgtgcgta agctaaattg tattttgtat
     2701 tatcgtcggt aggtttatcg cgcaacgatt gtttatatgc atttatactt tcttcgtatt
     2761 ttttttgctg caagtaagca ttacctaaat tatgatatgc atttgctctt gtttcagggt
     2821 cggaggcagc ctgagcagca cgttcatatt gtgcagcagc tttatcatat tcctgttgct
     2881 ggtaataagt attaccaaga ttataattac ctgcatcgga atcaggagtt tccgttaccg
     2941 cctgttcata tttttgttga gcacctttgt aatctttttg ttcatataag tcgttaccct
     3001 ccttaataat agcattacta ttttgggcaa aaccagtgta gcttattagt aacaaaaagg
     3061 ttaaaacaac aattctcatg gtttcatttt ttttcatcaa acaaatttac tttttccaac
     3121 cattttggtt ttttgtcgga cataaaaaac tctaacgtca ataaaaataa gccaattcct
     3181 aaaaataatt gaaaatgatt ggcatattcg gtaaactgat aggtatcatc tgccgtttta
     3241 tctaaggcat ctatttgctc gtatacgtcc tgtacaacct ttttcccgcc atttaaattc
     3301 atataaaccc catcggcttc tagtgcaagt tgttgcaaca tcttttcgtt gagtttggtc
     3361 aagacaacat taccttccct gtccttttta aatccattta cggtattgcc ctttctcaca
     3421 ggaatgggag caccggttgg tgtgccgacg cctaatgtaa ttattttaat atcgttatct
     3481 gctgcttcct ttgccatggc ttcggcatta tcatcatgac tttcaccatc ggtaatgata
     3541 ataattgctt tgcctttttt attatccttc tcaccaccat tgtcgaatgc cttgatagca
     3601 gtttctaaag cgctgccgat agcagtaccc tgattggtta tagttccggt attgatatta
     3661 tttaaataca tgagtgcagc acgcgaatca cttgttagtg gcatttgcac ataactttct
     3721 cctgcaaaaa caattaatcc tattttgttg gaagcgagtt tttcgattaa ttgcgccgca
     3781 gataatttag cttgagccaa cctattcggt tttacatctt ccgcaagcat actgttcgaa
     3841 acatcgaaac aaatcattac ttctgcactt tcacctttta ctttttgttt tttagaaccc
     3901 aatcgcaaat tggccaatcc taaaataata aaagcgcaag cagctaagag caatgaaaat
     3961 tttatccatt ttcttttcat ggaagttcta ttcatgagtt gctccaacag gtgttggtca
     4021 cccatttttg ccaaagcctt tttgcgccac gccacataag cataaaatac cagcatcagc
     4081 agcggtatta cagccaaccc atataacatt attttatttt cgaattccat attacgtgag
     4141 cgacctgaaa atagtattgg ttaaaagtat ttctatcaac aataaaataa atccaatcaa
     4201 agcaaaataa aagaaatgtt catcataacg ttgtgtgatg tccacttcaa acttttgttt
     4261 ttctatttgg tctatttgct gataaatttc tgataatttt tcttttgatg tagctttgaa
     4321 atattgtccg cctgttaggt ttgctacttc ttttaacatg gtttcgtcaa acgaaatgcg
     4381 cgggtccagt tcaaatattt ttcttccatt tttatctact acaggaattg gtgtattaga
     4441 ttctgaacca acaccaatgg tatacacgcg aacatttaat tgtttagcag cattagccgc
     4501 atcaaccggt gaatattttc cgccaatacg tacgccatca gttagtaaaa taatcacttt
     4561 tgtatttaca ttaaccgtat cggctaaacg atttactgct aaaaataatc catcaccaat
     4621 agcagtccca tctttaagat accagttatc ggcagtggct acctgggctt tcaacatttc
     4681 gtggtctaat gttgctgggc attgcgtaaa tgcttcaccg gcaaatacta ctaagccaat
     4741 gcggtcgttg gggcgactat caataaattc gttggcagcc acctttgctg cttccatcct
     4801 attcggttta aaatcgatag catacatact tggtgaaacg tcaagcgaaa gcataatatc
     4861 tataccctgg gacgaaattt ttttattgct aaatccggtt tgaggcctgg cgattgcaat
     4921 aattaataat attacagcag ttatacgcaa aacgggaagc aaacgcatcc attttatttt
     4981 agccgggaca taatctttaa atcctgaagt atttgaaatt ttaaatgcaa catatttatt
     5041 gtttgcctgc gtacgcaaat aataaatata caccggcacc agcagtagca gtaaaaggag
     5101 ccatggatcc tcaaatttta tattgctgat aaattcgttc atattttttc cggtgttggg
     5161 ggaataattt tagtattttc tacaaagttg acagttaatt ccatggccct cacattttca
     5221 tttgccaatg gtcgcgattt tgcaaatttt gccatatcgg ctaattgcaa aataaatgag
     5281 atatcattgg tgaaagcctt agcttccgga atatgttgta tttgttcaat aatttcatca
     5341 ctggtgcttt ccatggcatt gatataaaaa cgttgctcca aataatcgcg taaaatgtca
     5401 gttaattcag aataataaaa cttaagcctg tcctgttgcc ataatttttt ttcgtcgagt
     5461 gcccgcaatt gttccaaaaa tatttcatga agcggacgta ttggtttagc taaaggttta
     5521 ggtgttggct tatcttcttt tctaataaag aaaaaataaa ctaaaaatgc gatggcaatt
     5581 atggcatgca aaagcaataa tatttgcatc catgtaaaat cgcgaactgt taccagtttt
     5641 atatctttaa tcggtttaaa agcagcggtg gtgtcaacct caggagtgat gacattaaat
     5701 ggcaacgaat cagacattgc taataacgtt gttgtatcac gctgcatttt atataggata
     5761 aatacctggg ggaaataata ttttccttct tcgtaaatac tataggttat ttgctgcgta
     5821 aacattgttt taccatcagc agttagtgta tcaattggct gggcagaaat aatttcatac
     5881 ggcgcaattt cttccaccgg tggccaataa taggtattgg tgctatcgcc ggttgcttta
     5941 atggtaattt gtatataatc gccaatggca taatcaaact tatcggtttg cacttctaca
     6001 cttacctggg ctgttaatgg cacaagtaaa agacaaaaca aatgatatgt aataattttc
     6061 ctcagcatac tatttcatca taattctttt tctgaaaaaa ctatggagtt cgttgatata
     6121 tggtttctgg ctgtgaatag atatggtatc ggcaccactt tttacaaatg tggatttaaa
     6181 tgcctgcaca ttttttaaat aattacttcg gtacctatct ctcacttttt tattggaagt
     6241 atccaccaaa attgtgtcat ttttttcggg gtcaaacatt ctaacaattc ccgcatcagg
     6301 taattcctct tcacgtttgt cgtggatatg tacaccaatt acatcgtgtt ttcgcgccgc
     6361 aatcatcaaa ggtttttcat aacccttatc cataaaatcg ctaaaaacaa atgcaatact
     6421 ttttttattc accacattat taaaatattg taatgcctgg gcaatatttg ttccttttcc
     6481 ttcaggagtt agttcaagca aatcactgat aattcgcagg atatgtgatt ttcctttttt
     6541 gggtggaata aatttttcga tgcggtcgga aaataaaatc accccaactt tatcgttgtt
     6601 attaatagca gaaaaactta atacagctgc aatttctgta atcagctgat ttttcatttg
     6661 tagctgtgtg ccaaaaaatg agctctgact gatatctatg agcaacatta cggtaagttc
     6721 acgttcctct tcaaatactt taatataggg atggctcatg cgtgcagtaa cattccattc
     6781 aatattgcgc acatcatcac caaaagtata ttcgcgcact tcgctaaaac tcattcctct
     6841 tcctttaaac gcagagtgat attccccgga aaaaatattc tgggaaatac ccttcgtttt
     6901 tatttcgatg agtcgaactt tttttagcaa ggttgcttta tccaattgtt ttactcgtta
     6961 aactgtgaat taaatacatg ttatgtgtag tacacacatt aaaacattta aggcacctca
     7021 actacattga gaatttcggc aattatatct tctgcagtaa tattttccgc ttctgcttcg
     7081 taggttaatc caattcggtg acggagcaca tcctgacaaa ttgcgcgcac atcttccgga
     7141 ataacataac ctctgcgttt aataaatgca tacgctttac ttgccattgc caagttaatg
     7201 cttgcacgtg gcgaaccacc gtaagcaata agatgtttga ttttcgataa tttataatct
     7261 tcaggtttgc gtgtagcaaa aacgatatcg agaatataat tttctatttt atcatccata
     7321 tacacttcgc gtactaaatc acgggctctg ataatatctt caggtttaac tacctgagca
     7381 attttgttga ttgcgccggt gacattttga cgtataatta atttttcatc ttctttactt
     7441 gggtaatgaa ttaccacttt tagcataaaa cggtctacct gtgcttccgg taatggataa
     7501 gttccttctt gttcaatcgg gttttgtgta gctaacacta aaaatggctc ttccagttta
     7561 tacgtgtttt ctccgatggt aacctgtttt tcctgcatcg cttcaagtaa agcactttgt
     7621 acttttgccg gagcacggtt aatttcatct gccaaaataa aattggcgaa aatgggtcct
     7681 ttttttacgg taaactggtt gtctttctga ttgtaaatca tggttccaat caaatcggca
     7741 ggtaataaat cgggtgtaaa ctgaatgcgc gaaaatttcg cattgattgc tgaagccagt
     7801 gacttaatag ctaaagtttt tgctaagccc ggaacacctt ctaataaaat atgaccttgt
     7861 gtaagtaatc ccagtaacag gcggtcaatc atattttttt gccccacaat agctttggca
     7921 gtttccagcc ttaataaatc tataaatgcg ctttcctgat gaatgcgttc gtttaattct
     7981 ttaatgtcgg tagatgccgc agtatccata ttagtcaagt ttggtcgatt aaaattttca
     8041 gaacacaaaa ataactctgt gttcatacat taaatttggt gtgcaaagtt aattaaggtt
     8101 aactacaaga taaacattgt taaccgcaag ctttataaca agtgaacctt gattaacgta
     8161 gctttaggac actttaaaac aaacgctatg caaaataaaa tcagcattca ggcaaaccta
     8221 ggcaatgtgc agtatcgcac gacactcagc aatggtaaac atacactttt ggccgatgag
     8281 ccggagagtg aaggcggact ggccactggc cccactccgg atgaattttt agccatggct
     8341 ttggcagctt gtaccagcat tacactgcgt atgtatagtc agcgaaaaaa ttgggatttg
     8401 ggcgaaattg ctgtagcagt tgatatgcaa aggcaggatg acggttcagc tgtatttgaa
     8461 cgtaaaatca ccttcgaaaa aaaacctgat gcagccacct gcgaacgttt gatgattgtt
     8521 gccgataaat gtcccgtgca taaaacttta tcgcatgtta ataccattat ttcatcctta
     8581 taaacaacat tatgaacgat atagtttcaa gtaatgaaat taaaatggta attgatacat
     8641 tttatgaaaa agtgcgcgcc gacgagatga ttggatttat ttttaacgat atagcaaaag
     8701 tagattggga acatcacatg ccaataatgt ataacttttg ggaatcgaca gtttttggta
     8761 caggcacgta tacaagaaat gctatgacac ctcattttgt attgcaggat aaagtcaaat
     8821 ttactgcagc atattttgaa cgctgggtgt ttttatttgt gactactgtc gacgaattat
     8881 atacaggaga aaatgctgag ataatgaaaa tgcgtgcaac tagtattgca ggtttaatgc
     8941 aaaataagtt aggtataaat tgaataccac aacctgattt aatctgtatt tatgatattt
     9001 aatgcgctat aaataacagt cagttttata gcgggctgag tttataaatt atttcataac
     9061 tcgctgatta tcaaaacaat aatattacat ttttattgaa attatagcta gataaacgac
     9121 tgccaataac ttttttggta gttgtaaaat tcattatacc aattttctaa aaccgtttaa
     9181 ttattttact tttgtataaa caacaggcta cttatgacaa ttatactcac cattttaatg
     9241 attatcggaa ttattatttc ggttatttta attacagcat taatactacc taaaaaatat
     9301 aaagttttac gcgaagccac tattcaacaa ccacaacaaa aagtatttga ttatatcaaa
     9361 tacattcgca atcaggatgg atttagcgta tgggcgaata tggacccaaa catgaaaaaa
     9421 acgtataaag gtgacgatgg aagagttgga tttgtttcag cgtgggaaag cgacaataaa
     9481 aaagttggtg caggtgaaca ggaaataatt cgcgttgatg aaggtaaagg cattgttact
     9541 actttgcgtt tttttaaacc atttccggga gaagcggtgg ctcaatttat gacctcgccg
     9601 gcagaaaata atgctacaaa agtttcctgg caattagaca gtgccatgaa atatccgatg
     9661 aacttcatgt tgttgtttat gaacatggat aaaatgttgg gtgccgatat ggaaaaaggc
     9721 ttggaaaatt taaaaaataa attggccgaa taagtttaat cccaaccggg ataaatcacg
     9781 aaattatttt ccgtttcata aagctcgatt tttacatcaa attcagcatc cagttttgcc
     9841 ctgaatttgt cgtaaataac cttggctata ttttcagctg tagggtttaa cgctgcaaat
     9901 tccggcacat ctaaattcag gttattgtgg tcgaaaggct caataaccag ttctttaatc
     9961 aggtcgttga caattttcaa atccattaca taaccagttt gcggatttat ttcgccggta
    10021 attttcacaa aaagggtata gttatgcccg tgaaagttag ggttattgca taagccaaac
    10081 accttgtcgt tagtggcttc atcccaatcg gggttaaata atcggtgggc tgcgttaaag
    10141 gtagctcttc tgcaaactgt tgttctcata atgcctgaac aattaatgtt gtgcaaaggt
    10201 tgccaaaata ctttaaattt gccctattgt gacgatggta gtgacagatt caattcatgc
    10261 attcacgttc cgttcaattg atggcggaac gatagatttt tcagcattta agggcaaaaa
    10321 agtgcttgtt gtgaacaccg caagcgattg tatgtatacc gatcagtata aacaattgca
    10381 ggaattacac gacaattatg ctgataaact ggttattgtt ggttttcctt cgaatgattt
    10441 tggtgagcag gaaccgggaa cagaccgaga aattaaaaat ttttgttcct ataaatacgg
    10501 agtaacgttt ccacttgcct cgaaaagcga agtaattggt gtaaacagta atcctatatt
    10561 caaatggctg attagtttgc cgtttgaaaa tacgctcaac aagtcgattg tatggaattt
    10621 ccagaaattt ttattgaatg aagaaggcaa actcattgct gtttttccgc ctgccaccga
    10681 tccgataaat gatgaactgc tagcatttct caacgaaaat tgagtagtgc cggccaaatg
    10741 aacaggcgtg ctatttaaaa atgtaattgg tcaaacccgc atcaaattgc agctcacaaa
    10801 aggtgtagct gacaatcggg tgagtcatgc ccagttgttt cttggtccgg aaggaagcgg
    10861 caatttagcc atggccttag cctattctca atatattaat
//
LOCUS       DAORKW010000055        10754 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_122_length_10754_cov_5.049537, whole genome shotgun sequence.
ACCESSION   DAORKW010000055 DAORKW010000000
VERSION     DAORKW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122_length_10754_cov_5.049537"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PLB46_03845"
     CDS             <1..537
                     /locus_tag="PLB46_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HRF75680.1"
                     /translation="DILKDSVIGLSLRVLGVEGARVKITPDSIEIIDRLNQEYIPRDI
                     DFIKSSFNIDADFYDLQNLIVGNPVYYDTTALNTGESDDKYVLLAENTVYKNTLWLSP
                     DYDILRMFIQDLIANRTLTLDYDAYEKIDGRQFAFIRNISIDAQDDLTAHIEFTKVEF
                     DQPFGFTFTVNPKYKRVD"
     gene            479..1921
                     /locus_tag="PLB46_03850"
     CDS             479..1921
                     /locus_tag="PLB46_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRF75681.1"
                     /translation="MTSHSGLPLLLTPNTNASTNTEANLITPISRFPFYGIVLIVIAL
                     LLAQGSTALAQTGDKASLQSKYNKLQNEIKDTEDLLNSTKKKKENSLNDLKLLNRKID
                     MREQVINNIAQQMHSVNQELNSTNAAINAMQSDMTELKKQYAQMVYYTYVNDQDYKPL
                     HFIFSSKSINEAFQRVQYIRSFHGFRKEQIVAIEEISEDLTKKIEIIEQEKAEKESLL
                     SKEEKEKKQLDSEKAKKDEAVKKLQTQEKDLKKQIDKKKKDAASLKNKIQAIISEEIR
                     KEKEKAAAEAARIKEAEAKKALEQKNTNTNTSTATTTKTTESTTTTKTAEKTTEKVNL
                     GLTPEMQLISKNFAGNKGKLPWPVERGTITERFGKHAHPVLKDVIVENNGIDIATTEG
                     TAVRSIFDGEVVNVIFNPSFQKGVIIKHGEYYSVYTNLENVTVKAGDKVTTKQKIGTA
                     WEDPEEGKTEVHLEIWKNTVIMDPALWISK"
     gene            2109..2591
                     /locus_tag="PLB46_03855"
     CDS             2109..2591
                     /locus_tag="PLB46_03855"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRF75682.1"
                     /translation="MEKPLINKKYLLQKYPGKGGWTYVVIPEVLQDKRAPFGWVKVKG
                     FIDDFEIKSYKLMPLGNGQLFLPVKSEIRKKIGKQAGAWVNVVLYADNTPLEIPEELL
                     VCLKEDPIAYKTFLSYTEGQQKEFIDWIYSAKTDDTKVRRIAATLNKLIKKEKLRDVK
                     "
     gene            2800..3456
                     /locus_tag="PLB46_03860"
     CDS             2800..3456
                     /locus_tag="PLB46_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75683.1"
                     /translation="MTDEQAVNITKDVFAFITTAKTISDLSQLPEHLSLISKHKLPIN
                     TYPKIEFSISEQEIQSLIKNEMLNKDHEFTSKINSSLTDPLTKLLYATVWKNGDLKKI
                     KHIIKGILDGANHNSQQSEALVFYQFGKYLTKVPGQPIIDQHVIRAFGIYKATNLAEI
                     TKLRHMKIIDKNHIDIIEAYKVWLTSDAIQNELKNNVDYTYHIDNLLFATGKTIKQHN
                     "
     gene            3666..5240
                     /locus_tag="PLB46_03865"
     CDS             3666..5240
                     /locus_tag="PLB46_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75684.1"
                     /translation="MKNKLWLFVSCLYLFFPNVTFSQITFQKTYNGAIASKGNSVIQT
                     SDEGYIVVGSSTQAFGLTYEDAYIIKTDKQGNVEWTTFYGGPFDEVAFSVRETDDGGF
                     IIAANTSSYGAGYWDIYLIKLDKNGNSEWTKTIGGNNDDRCYAIEQTIDGGFIIAGTT
                     LSFGTEYYNIYLIKTDSNGNEVWAKIFGGSGGDYAYDVQETEDGGFIITGDTQSFSAV
                     GYTDVYLIKTDNAGNLLWSKTYGGPSNEVGKAISQTTDGGYIIAGITQGFSAYPKSDV
                     YLIKTDESGNIIWTKTHGGESYDNATSVEQTNDGGYIIAGNTNRAVDGVFNCYLIKTD
                     EVGDVIWTNSFGSVEYYYGDYFAHQTIDGGFIISGTTYNYDGFYNAVYLVKTDALGNT
                     GCNGENISTVSDTPETQEYNTFGLDSNIISITSSPVDIYKNGDDTASTICFNEIETDN
                     SLWVYPNPCINEVIVVGTANDGELIIYNIAGQAVLRKETVTAQTIINTTQLIPGIYLL
                     NYSNGDKTTSIKLIKY"
     gene            complement(5404..5718)
                     /locus_tag="PLB46_03870"
     CDS             complement(5404..5718)
                     /locus_tag="PLB46_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75685.1"
                     /translation="MPFQFFGVYFQGDDDTEYRCGFHDNNHYMPILERRDASGWVEVN
                     QHNGTGSATQACRDIRSELPVVINFPAGHTQNERMELINQIVYNLNRYTTERVNYQGA
                     HT"
     gene            complement(5730..6395)
                     /locus_tag="PLB46_03875"
     CDS             complement(5730..6395)
                     /locus_tag="PLB46_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75686.1"
                     /translation="MALIENSKGRKSGGGYERLFGNQLLGHLLSRVQASVISSGNELE
                     HVIITHSKSIADVDSFLSQQELPPGTYLITKQAIKKSVLKSDQEPDLLVFEIDPPHRH
                     CYIIELKDGDNFDTKKAAGEVALLQKFENHISKKLQFTTSIHVCSFNQNDKDKIVAGF
                     KKKITTDMAMTGKELCDLLNIDYTEILGLRIGEQGKNFDYFISQLLEIPEAKEAILDK
                     LFE"
     gene            complement(6382..7131)
                     /locus_tag="PLB46_03880"
     CDS             complement(6382..7131)
                     /locus_tag="PLB46_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRF75687.1"
                     /translation="MKSETSSLNVYSKERNVVIKQLENVIIGDTIKTNEPNVMDGLTF
                     LDGIFSNSISVCFFDPQYRGIMDKMKYGNEGVSRQIERSQLSQMSEEVISEFIQEIGR
                     VLIPSGHLFLWVDKFHLCQGVASWLKDTDLSIVDLITWDKGKIGMGYRTRRKTEYLVI
                     IQKLPIKAKGIWTKHNIPDVWTEKIVGKIHPHQKPLELQKALIEAVTNEGEYVIDPCA
                     GSYNVMEACKQINRIFIGSDLRNPYQENGTN"
     gene            8220..8882
                     /locus_tag="PLB46_03885"
     CDS             8220..8882
                     /locus_tag="PLB46_03885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HRF75688.1"
                     /translation="MKNYSVDISKKWDYENGFYLTCETSRIGKFLNHLEIYNHILGIE
                     GDILEFGVYKGASLVRLLSFRNLLENESKRKVYGFDIFGKFPDSLALESDIQFVHNFE
                     NAGGYGISKSDLEMLLNKKGFNNYALIQGNILNTIPEFVANKISEKIALLHIDVDVYE
                     PTKVILENLWDKVVPGGVIILDDYGIVEGETKAVDEFFAGQHITINKPKFNNLPSFII
                     KK"
     gene            8966..10420
                     /locus_tag="PLB46_03890"
     CDS             8966..10420
                     /locus_tag="PLB46_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF75689.1"
                     /translation="MFKMKLIITSLIFSFGSAYCLAQNNNTKSIDVLKTETAAAIQSN
                     YDRYKNIALSIWDYAELGYKETKSSSLLQNTLTENGFTVTSGVAGMPTAFVATYGSGS
                     PVIGILAEYDALPGLSQDTSATKSPIENKPNGHGCGHNLFGTGSVAAGIAIKNLLASG
                     DLKGTIKVYGCPAEEGGSGKVYMVREGLFNDVDVVLHWHASDNNAVTYTSALANKSAK
                     FRFYGVASHASVAPEKGRSALDGVEALNYMTNMMREHIPQEARVHYVITNGGKAPNVV
                     PEFAEVYYYVRHPDKATAVAIFDRIVKAAEGAALGTETKMDYEIIGGTHDLLINKTLA
                     INMLSNLEKVGGVTYTTEEIAFAKTIQSGFTGIIPPINSAEKILPLQIESALGSTDVG
                     DVSYVVPTVGVNTATWVPGTVAHSWQAVACGGTDIGIKGMMVASKTMALTAIDLFTNP
                     ELIKKAKEEFILSKGDYQYRALLGDRKPALNYRD"
     gene            10593..>10754
                     /locus_tag="PLB46_03895"
     CDS             10593..>10754
                     /locus_tag="PLB46_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF75690.1"
                     /translation="METRRDIFQAIADPTRRAILTLLAFQAMTPGAIASNFDSKRQTI
                     SKHIQILTEC"
BASE COUNT         3723 a         1836 c         1883 g         3312 t
ORIGIN      
        1 gatatcctga aggactccgt aattggcctt tcactccgtg tgttaggtgt agaaggcgca
       61 agggtaaaaa tcacccccga cagcatagaa ataatcgaca ggcttaatca ggaatatatt
      121 ccccgcgaca tcgattttat taaatcctcg ttcaatattg acgccgattt ttacgattta
      181 caaaatctga tagtcggcaa tccggtttat tacgacacta ccgccctcaa taccggtgaa
      241 agtgacgaca aatatgtact attagcagaa aatactgttt ataaaaacac actttggttg
      301 agccctgatt atgacatctt acgtatgttt atccaggatt taatcgcaaa tcgcaccctt
      361 acgctggatt atgatgcgta tgagaaaata gatggaaggc aatttgcgtt tatccgtaac
      421 atatctatag atgcacaaga tgaccttaca gcgcatattg agtttaccaa agtcgaattt
      481 gaccagccat tcgggtttac ctttactgtt aaccccaaat acaaacgcgt cgactaatac
      541 agaagcgaat ttaattacac cgatttcgcg ttttcctttt tatggtattg ttttaattgt
      601 aatagcgttg ttgcttgcac aaggttctac tgcgttagca caaaccggcg acaaagcaag
      661 tttgcaatca aaatacaata aactacaaaa cgaaataaaa gacacagagg atttactcaa
      721 cagcacaaaa aagaaaaaag aaaattcttt aaacgatttg aaactattga atcgcaaaat
      781 tgacatgcgt gagcaggtga taaataacat tgcacaacaa atgcatagcg ttaatcagga
      841 attaaacagc accaatgcag ctattaatgc catgcaaagt gatatgaccg aattaaaaaa
      901 gcaatatgct caaatggtat attacacgta tgtgaatgat caagattaca aaccattaca
      961 ttttattttt tcgagtaaaa gtattaatga agcatttcaa cgtgtgcaat atatccgttc
     1021 gtttcatggt ttcagaaaag aacaaattgt tgcaatagaa gaaataagtg aagatttgac
     1081 taaaaaaatt gaaattattg agcaggaaaa agcggaaaaa gaatcgttgt tgagcaaaga
     1141 agaaaaagaa aaaaagcaac tcgatagtga aaaagcaaaa aaagatgagg ccgttaaaaa
     1201 attgcaaacg caggaaaaag atttaaagaa acaaatcgat aaaaagaaaa aagatgcggc
     1261 ctctttaaaa aataaaattc aggcaattat ttcggaagaa atacggaaag aaaaagaaaa
     1321 agcagctgct gaagcagccc gtataaaaga agcagaagca aaaaaggctt tggagcaaaa
     1381 aaacaccaat accaatacca gcacagcaac aactacaaaa accacagaaa gcacaactac
     1441 caccaaaacc gccgaaaaaa caacagaaaa agtcaatctg ggcttaaccc cggaaatgca
     1501 actcatttca aaaaactttg ccggcaataa aggtaaatta ccatggccgg tcgaacgtgg
     1561 aacaattacc gaacgttttg gaaaacatgc gcatccggtt ttaaaagatg taattgttga
     1621 aaataatggt attgatattg ccacaactga aggcactgca gtgcgcagta tatttgatgg
     1681 tgaagtagta aatgtaattt ttaatccttc gttccaaaaa ggcgtaatta taaaacacgg
     1741 cgaatattac agcgtataca ccaatctcga aaatgtgacc gtaaaagccg gcgacaaagt
     1801 aactaccaaa caaaaaatag gcacagcctg ggaagacccc gaagaaggca aaactgaagt
     1861 gcacttagaa atctggaaaa atacagtaat tatggatcct gcgttgtgga ttagtaaata
     1921 agttttgcat gagggatgag tgatgagttg tatccaacaa ttggggattt aatactgcta
     1981 tttgaaatta tggaatgact atttattcat accgttgcac gtttggaaat aattgtaaat
     2041 tcgattagaa ttaattcgca gaatttgatt agtgatacct aaccttttaa agtaaaatag
     2101 taagggaatt ggaaaagcca ttaataaaca aaaaatattt acttcaaaaa tatccgggga
     2161 aaggcggttg gacatatgtt gtaattcccg aagtgcttca ggataagcga gcaccttttg
     2221 gttgggttaa ggtaaaagga tttattgatg actttgaaat aaaaagttac aagctaatgc
     2281 cattgggtaa cggtcaactt tttttacctg ttaagtcgga aatcagaaaa aaaattggta
     2341 agcaggcagg tgcttgggta aatgtggttt tatatgctga taacacacca ttagaaatac
     2401 cggaagaatt gttagtttgt ttaaaggaag atccaattgc ttacaaaaca tttttaagtt
     2461 atacagaagg tcaacaaaaa gaatttatcg attggattta ttcagcgaaa accgatgaca
     2521 caaaagttcg gagaattgca gcgacgctta ataaattaat aaaaaaggaa aaactaagag
     2581 acgtgaaata acaatattag tagattagaa aaattatcgt ttcctaacgc taattttgaa
     2641 atccaaaaat tagtggtttt gtggtaaggt atattaaagc acagggtaaa aagtatagac
     2701 aattaatgat aaattgttta atttgtctat tatttacatc ttgtaccaat ttatggtgct
     2761 aacatacctt tatcagttaa atcatttata aacaccaaga tgaccgacga gcaagcagtt
     2821 aatattacaa aagatgtttt tgcgtttatc acaacagcta aaacaatttc agatctatct
     2881 caacttcctg aacacttaag tttgataagt aagcataaac taccaattaa cacctaccct
     2941 aaaattgaat ttagcataag cgaacaagaa attcaatcat taataaaaaa tgagatgctt
     3001 aataaagatc acgaattcac atcaaaaata aatagtagtt taacagaccc tctgacaaaa
     3061 ctactatacg ctaccgtatg gaaaaatggt gacctaaaaa aaataaaaca tatcattaaa
     3121 ggtattttag acggtgcaaa tcataacagt caacaaagcg aggcattagt attctatcaa
     3181 tttggcaagt atttaactaa agttcctgga caaccaatca tcgaccaaca tgtaattcgg
     3241 gcatttggta tatataaagc aacaaattta gccgagatta caaagttaag gcatatgaaa
     3301 attatcgaca aaaatcatat tgatattatt gaagcctata aggtttggct tacttccgac
     3361 gccatacaaa atgaactgaa aaacaatgtt gattacactt accacatcga taatttgtta
     3421 tttgcaaccg gtaaaacaat taaacaacat aattaaaaca actaaattaa gtaacctaat
     3481 tagccatctt taaacaatta atataatgcc caattattaa ggtgtttttg ttaattccaa
     3541 cgaaattgat tgaaataaac aaaactaaac cgatgttaac gactattaag ggccattgaa
     3601 tattggttga aatacatttt acaccacatt attttgtata attgttgagt tttaaagtgg
     3661 tgcctatgaa aaacaaactt tggttgtttg ttagttgctt gtatttgttt tttccaaatg
     3721 tgacattttc tcaaattaca tttcaaaaaa catataacgg agcaattgca agcaagggga
     3781 actcggtcat tcagacttca gacgaaggtt atattgttgt tggctcaagt acacaagctt
     3841 ttggcttaac ctatgaggat gcctatatca ttaaaactga taagcaaggt aatgtggaat
     3901 ggacaacttt ttatggcggg ccatttgacg aagttgcttt ttcggtgcgg gaaactgatg
     3961 acggaggatt tattattgct gctaatacta gtagctatgg tgccggatac tgggatattt
     4021 atttaattaa attagataaa aatggcaatt cggaatggac caaaacaata ggaggaaata
     4081 acgatgatcg ttgttatgca attgaacaaa ccattgatgg aggatttatt attgcaggta
     4141 ctacattgag ttttggaacc gaatattata acatttatct tattaaaact gacagcaatg
     4201 gtaatgaagt ttgggcaaaa atttttggcg gaagtggcgg tgattatgcc tacgatgtgc
     4261 aggaaaccga agatggaggt tttatcatta ccggtgatac gcaaagtttc agtgcagttg
     4321 gatatacgga tgtttacctt attaaaacag ataatgcagg caatttgctt tggtcaaaaa
     4381 catatggtgg ccccagtaat gaagttggga aggcaatcag tcaaaccaca gatggcggct
     4441 atattattgc cggtattaca caaggatttt cagcttaccc caaatctgat gtttatttga
     4501 ttaaaacgga tgaatccggg aatatcattt ggacgaaaac acatggtgga gaaagttatg
     4561 ataatgccac ctctgtagaa caaacaaatg atggcggcta cataattgcg ggaaatacca
     4621 atagagcagt tgacggtgtt ttcaactgtt atttaattaa aacagatgaa gttggagatg
     4681 taatttggac aaactccttt ggatctgttg aatattatta tggcgactat tttgcacatc
     4741 aaacaataga tgggggattt atcatatcag gcactactta taattatgac ggtttttata
     4801 atgcagttta tttagtaaaa accgatgcat tgggaaatac cggttgcaat ggagaaaata
     4861 tcagcactgt atctgataca cccgaaacgc aggagtataa tacgtttgga ttggattcta
     4921 atattataag tattacgtct tcgccagttg atatttacaa aaacggagat gacacagctt
     4981 ccactatttg ttttaatgaa attgaaaccg acaattcact gtgggtttat cccaatccat
     5041 gtataaacga agtaatagta gtaggaacag caaatgatgg tgaattaata atttataaca
     5101 tagcaggaca ggcagtcttg cgcaaagaaa cggttactgc acaaacaata ataaatacaa
     5161 cacaactcat tcctgggata tatttattaa actacagcaa tggagataaa acaacaagta
     5221 tcaagttaat aaaatattaa tattcttgtc gagcaaccca attgttcaca attttaggta
     5281 caaaatgaat gccctaacta taaaaaaagt aattaagcta tatttttata cgcttgcatt
     5341 ttataaggca caaatttttc attgacctta tttattattt acattacact ttggaaaaat
     5401 atgttatgta tgagctccct gataatttac acgctctgtt gtgtacctgt taagattata
     5461 tacaatttga ttaattagtt ccatcctttc gttttgtgta tgtccggcag ggaaattaat
     5521 tacaacaggc agttctgatc gaatatctct gcatgcctgt gtagcactcc ctgttccatt
     5581 gtgttgattg acttctaccc aacccgaagc atctcttctt tcaagtattg gcatgtaatg
     5641 attgttatcg tgaaatccac atcggtattc cgtgtcgtca tccccttgga aatagacacc
     5701 aaaaaattga aaaggcataa gtattgattt tattcaaaga gtttatcaag tattgcttct
     5761 tttgcctctg gtatttccag tagctgactt ataaaataat caaaattttt accctgctca
     5821 ccaattctta atcctaatat ttccgtgtaa tcgatattta ataagtcaca aagttcttta
     5881 cctgtcattg ccatatctgt cgttattttt ttcttaaacc ctgccacaat tttatccttg
     5941 tcgttttgat taaatgaaca aacgtgaata gaagtggtaa actgcaattt ttttgatata
     6001 tgattttcaa atttttgcag caaagcaact tctcctgcgg ccttcttagt atcaaaattg
     6061 tcgccatcct tcaattcaat gatataacag tgtcgatgcg gtggatcaat ttcaaatact
     6121 aataaatcgg gctcttgatc tgatttgagt acagactttt ttattgcttg tttagtaatg
     6181 agataagtac ctggtggtaa ttcctgttga gagaggaagc tatcaacatc cgctatgctt
     6241 tttgaatgcg ttataattac atgctcaagt tcattcccgg aggagattac agatgcctga
     6301 actctagaaa gtaaatgtcc taataactga ttaccaaaaa gtctttcgta acctccgccc
     6361 gatttgcgtc ctttactgtt ctcaattagt gccattttct tggtatgggt ttcttaaatc
     6421 cgaacctatg aatatccggt ttatttgttt gcaagcctcc attacattat aacttcctgc
     6481 acatgggtca attacatatt caccttcatt agttacagct tcgatcaacg ctttttgcaa
     6541 ctcaagtggt ttttgatggg ggtgaatttt tccaacaatc ttttctgtcc aaacatccgg
     6601 gatattgtgt ttagtccaaa ttccctttgc ttttattggt agtttttgaa ttataacaag
     6661 gtattctgtt tttctccgtg ttctatatcc cattccaatt tttcccttat cccaggtaat
     6721 aagatcaact atcgataaat cagtgtcttt taaccaactt gccacacctt ggcataaatg
     6781 aaatttatca acccataaaa acaaatgccc actcggaata agaacccgtc caatttcttg
     6841 aataaattct gaaataactt cctcagacat ttggcttaat tgagaccgtt caatttgcct
     6901 gcttacgccc tcatttccgt atttcatttt atccataatt cctctatatt gaggatcaaa
     6961 aaaacacact gaaattgaat tactaaatat accatccaaa aatgtaagcc catccattac
     7021 attaggctca tttgttttga tggtatcacc tattatgaca ttttcaagtt gcttaattac
     7081 aacattcctt tctttggaat aaacatttaa tgatgacgtt tctgatttca cacttccagt
     7141 actcatttaa attgatttat taagtggtgc tctatttcgc aatcctttca attgattaac
     7201 taatttattt tttactttaa aactgtgtta taatattttt ttcattaaaa acactataag
     7261 cagccatgtg aaaacctaac aaatactttt cactattttc taattttaca cccggctttc
     7321 tataattcaa catgctttaa aacttagaaa acatgcaaat tttcaaccaa aattaacgtt
     7381 ttcaatctat cacctgctat ttgctggcaa atcaacatat tctaggcatg gtggtataaa
     7441 tttgcttttc atagtagtct tattgaccga ttgttcccta ccgaataaaa accttatctg
     7501 ccaatttgta ggtgggacgc atatttcaga gctaccttac tacaagttgg tttaaacgca
     7561 attagcatca aacttatcat tactactttg attttttgcg aaattacaca ttactttaga
     7621 ttccaaacaa cggcaaagaa caagttggct atcgcaatat taacccacat gttacactgc
     7681 tttcagcata aatatcggat atcttttggt aaaattttca ctattacact tcattttttt
     7741 aaatttttcc gcaaagcgca gtaaaataat actaaacaag caaagaaacc tattgatttc
     7801 acactcggtg tcctatacaa gtgctttacc aattgttgtc attaccttgt aaattaagcg
     7861 gtttcccaat tttaagctta ataattgaat atgattatga actttcaaaa cctaagggca
     7921 atattagata aaatcaaatt attaggattc taagttgccc actgttatgt tcaatgatgt
     7981 ggtataaaca tggcaaataa atcctgattg aatcaaatat aggcagtgtc gtggaacaac
     8041 acttcctttt agggactaaa ctaaaaccgt ttattaataa atgcctgtta atcaaagcac
     8101 cccatccccc aaacacctgg ggtattatct cctcaaaaat tctgactaac tttagcgctg
     8161 ccttttaaag gtcaattcaa caaatcaggc atcgtcattt taaaacaaaa cagcttgcta
     8221 tgaaaaacta ctcagtagac atatcgaaaa aatgggatta cgaaaatggg ttttatttaa
     8281 cttgtgaaac gagtagaatt ggcaaatttc tcaaccatct ggaaatctat aatcatattc
     8341 tcggaattga aggtgatatt ttagaatttg gtgtttacaa aggcgcttct ctggtgcgcc
     8401 ttttatcatt cagaaattta ttggaaaatg aatcaaaaag aaaagtatac gggtttgata
     8461 tatttggcaa atttcctgat tcattagcac ttgaaagtga tatacaattt gtccataatt
     8521 ttgaaaatgc cggaggttat ggtatcagca aaagcgacct ggaaatgtta cttaataaaa
     8581 aaggatttaa caactatgct ttaattcaag gcaatatttt aaataccatt cctgaatttg
     8641 tggcgaataa aatttccgaa aaaattgcct tattacatat cgatgttgat gtgtatgaac
     8701 ccactaaagt gatattagaa aacctttggg ataaagtggt gccgggcggt gtaatcattt
     8761 tagatgatta tggcattgtt gaaggtgaaa ctaaagcagt agatgaattt tttgccggtc
     8821 aacatatcac cattaataaa cccaagttta ataatctccc ttctttcatt attaaaaaat
     8881 aattgaaaat agcgcttact aagcattcct gtttatttct tttgataaca tcatttaaaa
     8941 aaattttaaa ataaaatatt attttatgtt taaaatgaaa ttgataatta catcccttat
     9001 cttttctttt ggcagcgctt attgtcttgc tcaaaacaat aataccaaat caattgatgt
     9061 tttgaaaact gagacagcag cagcaattca gtcgaattac gataggtata aaaatatcgc
     9121 gttaagcatt tgggattatg ccgagttggg ttataaagaa accaaaagtt caagtttatt
     9181 acaaaacaca ttaactgaaa atggctttac agtaacatca ggtgtggccg gtatgccaac
     9241 tgcttttgta gcaacctatg gcagtggctc accggttatt ggcatattgg ccgaatacga
     9301 tgccttgccc gggttatcgc aagatacttc tgcaacaaaa tcaccaattg aaaataaacc
     9361 aaatggtcac ggttgtggtc acaatttatt tggtacgggt tctgtagctg ccggaatagc
     9421 catcaaaaat ttattggctt cgggtgattt aaaaggtacc atcaaagtat atggttgccc
     9481 tgcagaagaa ggtggaagtg gcaaagtata tatggttcgt gaaggattat ttaatgatgt
     9541 tgatgttgtt ttacattggc atgcttccga taataatgca gtaacatata ccagtgcatt
     9601 ggctaataaa tctgcgaaat tcagatttta cggagttgca tcacatgcat cagttgcacc
     9661 cgaaaaagga cgttcagctt tggatggtgt tgaagcatta aattatatga caaacatgat
     9721 gcgtgaacat attccgcaag aagcacgtgt gcattatgtg atcaccaatg gtggcaaagc
     9781 acccaatgta gtgcccgaat ttgctgaagt gtattattat gtgaggcatc ccgataaagc
     9841 tacagcagtt gctattttcg acagaattgt aaaagctgcc gaaggtgctg cattaggaac
     9901 ggaaacaaaa atggattacg aaattatcgg tggcacacac gatttactta ttaataaaac
     9961 attggcaatt aatatgttat ccaatttaga aaaagttggt ggtgttactt ataccacaga
    10021 agaaatagct tttgccaaaa caatacaatc cggatttacc ggcattattc cacctattaa
    10081 cagtgcagaa aaaattttac cacttcaaat cgaaagtgca ttgggctcaa ctgatgttgg
    10141 cgatgtaagt tatgttgtgc ctaccgttgg tgttaatacc gccacttggg tacctggcac
    10201 tgtagcgcac agctggcagg ctgttgcatg tggtggaacc gatattggaa ttaagggaat
    10261 gatggttgct tcaaaaacaa tggcactaac tgcaatcgat ttatttacaa atcccgaatt
    10321 aattaaaaaa gccaaagaag aatttatttt gtctaaaggt gattatcaat atcgtgcttt
    10381 gttaggtgac agaaagcctg cattaaatta tagggattaa ctttttgttt tggtttatac
    10441 tagttaatgg ttacgtttaa ttagttatcc taaatgcgct ctgcaattat ttctccttct
    10501 tcacttaaaa aaagtctgca ttttaaaaaa agtgccaaaa aacttgcgca gtcaaataac
    10561 tgcattatat ttgcagtcaa atagctgcat aaatggaaac tcgcagagat atatttcaag
    10621 ccattgccga ccccaccaga agggcaatcc tgactttact ggcatttcag gcgatgactc
    10681 ccggagccat agcttccaat tttgattcaa aaaggcaaac catttcaaaa catattcaga
    10741 ttctaaccga atgt
//
LOCUS       DAORKW010000056        10743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_123_length_10743_cov_6.802863, whole genome shotgun sequence.
ACCESSION   DAORKW010000056 DAORKW010000000
VERSION     DAORKW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10743
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123_length_10743_cov_6.802863"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..942
                     /locus_tag="PLB46_03900"
     CDS             271..942
                     /locus_tag="PLB46_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75691.1"
                     /translation="MKNLLTFILLAISLSSFCQTITVDTTFAQVGRTFIVRRQCVDEK
                     YIILSIYDNKALILKDTLDFIGPGGGFDLWDFDMDGNKDLLLTMIGNNPISFLYLFDI
                     TKNSFKNVEGFNDFPQAKAVKGQKNIYYSYHRSGCADANWVSDLFKIVNFETIQIGHI
                     YGKGCDFEVGENPPVIEIYKILDNDEEKEELIQKIPYSKGVEEYGDKWDFIENYWSNN
                     YKRFE"
     gene            1395..2282
                     /locus_tag="PLB46_03905"
     CDS             1395..2282
                     /locus_tag="PLB46_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75692.1"
                     /translation="MANKFSLLQYSTAAVALLGVTAASSQVVYTDIDPDVVIVEPDEM
                     FGIDLDDDGLNDFNFFNESFTTYIPYAGSALIHAIFVGAFDTMQNGIVGSTIYNSAGP
                     GYTYHFPFAIQPGEVIGTSKIFHNDNYQTMARTESFLSLPFTFHQGNWHPALWGYKLE
                     DFIGIRFTDETEIQRYGWIRCSVIDSGRTLIIHDYAYELQPNYPIIAGDTLSYVDIQD
                     PTSIVATVHSYNNIVHINLITHTPTLLTIVNLNGQNILAKTLYHQVEIVDMNYFPKGI
                     YLVTLKEGENLFTTKVAIE"
     gene            2466..2960
                     /locus_tag="PLB46_03910"
     CDS             2466..2960
                     /locus_tag="PLB46_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRF75693.1"
                     /translation="MARPGRPNKSDLRIDEMKLDIEKGHNEKVKQLLLEVGIDACDDY
                     LRTALIWSTFFNNIHLLNWVINNGANVNHQDKNGYSALHVAAQEKHFDCAQILIDNGS
                     NLELGDLHGNTALWTAVFNSRGDTRLVKLYVQNGANLDHVNKYQRTPRQMAEIIAGFD
                     MTSI"
     gene            3139..3714
                     /locus_tag="PLB46_03915"
     CDS             3139..3714
                     /locus_tag="PLB46_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF75694.1"
                     /translation="MEKIIKHFEKYLPLSDHEKLALIGRIKERKIKRKQLILQEGDIC
                     KYFTYVIEGCFKTYGVDNAGKEHNLLFTAEDDWITDIDSLHKERPSKLFIEAIEPSII
                     LQISKGDLWYLYNNYPKFDRNFRVIIEDKYIELQNRLLQTFSTTAYERYESFMEQYPN
                     LANRLPNTQIASYLGITPEFLSKIRNERTVK"
     gene            3832..4494
                     /locus_tag="PLB46_03920"
     CDS             3832..4494
                     /locus_tag="PLB46_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRF75695.1"
                     /translation="MNTSKTKVAIIGLGNIGTAIATNLVKGNHSVILASRDNEKTNTL
                     ASQLGELASAREITDAIKDADVVIPAIYFNALKEFFQTYSKELEDKIIVDVSNPIAPD
                     GNGGFKKIIGEHESAGKVLSDLIPKNAKLIKAFGTLGAGSLLNSAFNVPDNKVLFYAT
                     DSTNSIQQIEELITSSGFIPFNVGGIDQSIKIEVFGDLHEFGALGKPVTLAEAKNKIA
                     HP"
     gene            4505..4990
                     /locus_tag="PLB46_03925"
     CDS             4505..4990
                     /locus_tag="PLB46_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HRF75696.1"
                     /translation="MKNLIISAMLLVVLASCQNETKNQSNNSTQKTMEQSTEKLAIEK
                     LLFSYRDALNSSDVNKVLPLYTNDGVFMPSNAPSAIGQEQVKVSYEFVFSQIQLNIEF
                     YIDEIVVNGDYAFARTTSKGKTLIRANGETVPEENRELFVLQKTNGQWKISRYMFNKM
                     K"
     gene            5189..6145
                     /locus_tag="PLB46_03930"
     CDS             5189..6145
                     /locus_tag="PLB46_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75697.1"
                     /translation="MFPLLFLLFFSCQNTTNENKVSDVKTKEVVSDTVYTWTKLLDSA
                     AWKKNYNFQMFSINDTLWTFHPDRIWYSKNGKNWTKSVLENSINNLAFLDYVYFKGSV
                     YGLGYFSGNIEQFTFKPEIVKTTDFKSWTTISRTSNLPNRFFYHPFVFENKIWIVGGE
                     DKNTKYADIWNSEDGITWTKQKENLPFGKRSGSQIVTLNNNLFLLDNDVWRSIDGLNW
                     EKVADEIVKGEQIFGYAAQVFDNKIWLLGCNRNGQFSSQVLYSSDGKNWQTNTAPWLP
                     RGGIAATVHNNKIYMTGGKYGGTPNHPEFRYDNDVWTLEKRE"
     gene            complement(6636..9233)
                     /locus_tag="PLB46_03935"
     CDS             complement(6636..9233)
                     /locus_tag="PLB46_03935"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRF75698.1"
                     /translation="MKNLNKLRPSTWIIFICLQIFTLNISAQYCIPQHSFPMLDVIID
                     GVELGDIMNTGSGVAEENIGYSDYTELSTALIAGSTNTIYLDNKSWQYNYSVWIDFNQ
                     NMEFEEAEKLGTIVLTGVQSGSITFSIPADAINGETRMRVRAVHEPFGPWMNSNDPCL
                     PFLQGDTEDYTVLISEGIEHDLSIKKIISPVSAVELTEESITIAITNHTDTDFSDVNV
                     AYSIDGGPEVVETVPDVIPANSDFIYTFSEPANLAGLGCYAITLEVQHPDDGFPLNDN
                     LEANICNLNYVSGTKKWLIHSNIDGGAEALSGAPYFNTTNTTCMNTVFGEGNWTQEYF
                     ETVDPEILFSDSSCFIFLDGSYNHIVAMELFLNDNRQLMENWVAAGGKLYLNSSGSES
                     EGEHFYVDYGFDGTKIAMSYQVTDIKKKTGITHPVYEGPYTPVGTTFSGFYASNAVIY
                     GKNYDTLAHENIDGVLFGAPHNDIPCMIEKQWGIGKVIMSAWAPSQLIDPADKNMNLR
                     QNILVYLGDCNVVIPDDAGVINLPELVSSCNKSETEEIKITIQNYGGTTISAFPVAYK
                     INGGPEIMEIADVSIAPGFEAEYTFATTADLSVPGEYVFEIYTLLPDDIDISNDTFTL
                     SINAYASPIAMLPENITACDAVILDAGNPGTEYVWNTGEITQTITVTSSGTYSVSISD
                     PVSGCEISDTVNVTLEFAPEASFNYTSSGLTVFFENTSSGTGTYFWVFGDGDGSTEID
                     PTHTYATEGNYSVTLIVENDCGISEYNMLITIQVTPISNTDIRSINLFPNPADEFFIV
                     ELENLSGNEIVLEMYDLQGKQIISPFETQIINENALNVNISVLPTGIYVVKLIVGKTV
                     YLGRVEKLR"
     gene            9416..10591
                     /locus_tag="PLB46_03940"
     CDS             9416..10591
                     /locus_tag="PLB46_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRF75699.1"
                     /translation="MLPEPSFTIGSFVFLIISAQGLFLAVLLAVYNRSNVVANRTMAI
                     FVAAFSMILCYYVLYWTGYSCNFMWTKGWCSALPFLLGPLMYLYIIIIKNGELPTNYK
                     KHFIPFAVHLTYMLPFIYWFTTGPEVIATSQEPMNLFLQDKIYRHLNFVITSFQVFNA
                     LQFLSMFIYAFLLLRLLIKQQKEKHGSEHQQMKYRWYVRNISFYVLFSLCFSSYWITL
                     YFGISIDGLDYLVSGIMSLCIYLIGYGAFRQPLSLFEQKNAITRKKLPPADLILQSQK
                     LTTYLETQKPWLDSELNLNKLSELLQMPVHELSFLINSTLHKNFSELVHDYRIKEACR
                     LLLDESYSDSKILSIAFDVGYTNKATFNAAFKNKMGVSPSAYKLMAKGHGMNEAINL"
BASE COUNT         3644 a         1926 c         1880 g         3293 t
ORIGIN      
        1 ccataaaagc ctaaacactc actggacaat gacagcgaaa accttaacag acctgactta
       61 aacgacacga cagacagaac gccagctggt aacacgggtt ttgcaaaagt ggcggtgcag
      121 tgcttcgtat gacagttttt cgtaaatttg aagtttggta cttcgaatga agttcggagc
      181 taaaaatcgc caccttcgca aagcccgaaa ccgttagcga taaatttaac ccacactcaa
      241 ccataatcga taaatattaa catacaaact atgaagaacc tcttgacttt tattcttctg
      301 gcaatttcac tttcctcatt ttgtcagaca ataacagttg acaccacatt tgcacaagtt
      361 ggcagaactt ttattgttag acgacagtgt gttgatgaaa agtatataat actttctatt
      421 tacgacaata aggcactcat cttaaaggat accttggatt ttattggacc gggaggagga
      481 tttgatttat gggactttga tatggacgga aataaggatt tattgttgac tatgattggt
      541 aacaacccaa tttcttttct ttatttattt gatattacaa agaattcatt taagaatgta
      601 gaaggattta atgattttcc acaagcaaaa gccgtaaaag gtcaaaaaaa tatttattat
      661 tcttatcaca gaagcggatg tgcagatgcc aattgggtaa gcgacctttt taaaattgtg
      721 aactttgaaa caattcaaat cggacacatc tatggtaaag gttgcgactt tgaggtagga
      781 gaaaatccgc cggtaattga aatttacaag atattggata atgatgaaga gaaggaagag
      841 ttgatacaaa aaattcctta ttctaaagga gttgaggaat acggagataa gtgggacttt
      901 atagaaaact attggagcaa caactacaaa cggtttgaat aaaaaccagc cgctaacaac
      961 acatttgcgc cagcggggcg aggacgacca ccgaatattt ttcgataatc aaaaacttag
     1021 taattttatt aaagattttc gattgcctga cccgccttcg caaatccgca aaacgttata
     1081 ggatatggtc tatcacattc acagttgcaa aaagcaggtt gagaaataca caatctggtc
     1141 aactgacaga ctgaacaaga aaaacggatc aaataaatca aacgcttaca gtttatccta
     1201 atccggcaaa caaccagctt aatatttcaa catcagaaca aatcataaaa gttaagataa
     1261 tttcaatgac aggagctaaa atgtttgaag caattgataa ttgatatttc aatgctcagt
     1321 tatggcatgt atcaggtaat ttgtaatttt agtgatggaa gtagtgttac aaaacctttt
     1381 ataaaattgt aataatggca aacaaatttt ctttattaca atattcaaca gcagcagtag
     1441 ccctccttgg ggttactgct gcttcatcac aggtggttta taccgatatt gaccccgatg
     1501 tagtaatagt tgaacctgat gaaatgtttg gtatcgatct ggatgacgat ggattgaatg
     1561 attttaattt ctttaatgaa agttttacga cttatattcc atatgcagga agtgctttaa
     1621 ttcatgcaat atttgtaggc gcatttgata caatgcaaaa tggaattgta gggagtacta
     1681 tttacaatag cgctgggcca ggttatacct atcattttcc ttttgcaatt cagccaggtg
     1741 aggtgattgg aacttcaaaa atattccata atgacaatta tcaaacaatg gcgagaacag
     1801 agtcgttttt aagtttacct tttacttttc accaagggaa ttggcatcct gccttgtggg
     1861 gatataaatt ggaagatttc attggaatca gatttacaga cgaaacagaa atccaaaggt
     1921 atggttggat tcgatgcagt gttatagata gcgggcgcac cttgattatt catgactatg
     1981 cttatgaatt gcaacccaat tatccgatta tagctggaga tacgttaagc tatgtggata
     2041 ttcaagatcc gacttcaatt gttgcaacgg tccatagtta caataatatt gtgcatatta
     2101 atttaataac acacacacct actctgctaa caattgttaa tcttaacggg cagaatattt
     2161 tggctaagac attataccat caagttgaaa ttgtggatat gaattacttc cccaaaggaa
     2221 tttatttagt tactttgaaa gaaggtgaaa acttatttac aacgaaagta gcaattgaat
     2281 aatcgaaaga ccacatccta taacaaaagc tttgcaaaat ggcaagtgac gagtaattat
     2341 taatattcat cttcaaataa aatttataga taaccatgaa acatttcgtt ttgttgccgc
     2401 cacttcgcaa agcttctaaa cgttatgcgt cattttcaca gtacatcaac caaaacgaat
     2461 aataaatggc aagacccggt agaccaaata aatcagacct acgaatagac gaaatgaagt
     2521 tggacattga aaaaggtcat aacgaaaaag taaaacaact tttattagaa gtgggtattg
     2581 atgcttgtga tgattattta cggacagctt taatttggtc aacatttttt aacaacattc
     2641 acttattaaa ttgggttata aacaacggtg caaacgttaa tcatcaagac aaaaatggtt
     2701 actctgcact tcatgttgca gcgcaagaaa aacactttga ttgtgctcaa atacttattg
     2761 acaatggctc aaatcttgaa ttaggtgacc ttcacgggaa caccgcgttg tggacagcag
     2821 tttttaattc tcgaggtgac acaagattag ttaaacttta tgttcaaaat ggtgcaaacc
     2881 ttgaccacgt aaacaagtat caaaggacac caagacaaat ggctgaaata attgcaggat
     2941 ttgatatgac atcaatatag atgtaaaaaa cccacaaaac aaacgcaatt gcattggcac
     3001 cgcgattact acctcaacac ttattcaaaa acctaaaact ttgtattttt attagtgatt
     3061 tacgattgcc agacctgctg tcgcaaatct gcataatgtt ataggcaacc cctacaaacg
     3121 atagtaaaaa agtatcagat ggaaaaaatt attaagcatt tcgaaaagta tcttccgttg
     3181 agtgatcacg aaaaattagc cttaattggt cgcataaaag agcgtaaaat caagcgcaaa
     3241 caattaattt tacaagaggg tgatatctgt aagtatttta cttatgtaat agagggatgt
     3301 tttaaaactt atggcgttga caatgccggt aaagaacaca atcttttatt tactgccgag
     3361 gacgactgga ttacggacat tgacagctta cacaaggagc ggccttcgaa attatttatt
     3421 gaggccattg aaccttctat tatattgcaa atatctaaag gcgacctttg gtatttatac
     3481 aacaattatc cgaagtttga caggaacttc cgagtaatta tcgaagacaa atatatagag
     3541 ctccaaaacc gacttcttca aacttttagc acgactgcat acgaacggta tgaaagtttc
     3601 atggaacaat atccgaacct tgctaaccga ttgcctaata cccaaattgc ttcttacctg
     3661 ggaattactc ccgaattttt aagtaaaatt agaaacgaaa gaactgtcaa ataatattac
     3721 ccttaatcta cattaagaca atttcttaaa ctagtttata ggtgcaggca ctgcttacac
     3781 ctcatctttg tattgtcaat tttgacattg taaaaattca atacaaaagt tatgaataca
     3841 tctaaaacaa aagtagctat tattgggctt ggcaatatcg gcacagccat tgcaacaaat
     3901 cttgtaaaag gaaatcattc cgtaattctt gcttctaggg ataacgagaa aacaaacaca
     3961 ttagcaagtc aattgggcga acttgcaagc gcaagagaaa ttacagatgc aataaaagac
     4021 gcggatgttg ttattcctgc catttatttc aacgcattaa aagagttttt tcaaacttat
     4081 tccaaagaat tggaagataa aattattgtt gatgtatcta atcccattgc gcctgatggg
     4141 aatggtggct ttaaaaaaat tataggagag catgaatcgg ccggcaaagt tttatcggat
     4201 ttaattccta aaaatgctaa actaattaag gcttttggaa cattaggcgc cggctcatta
     4261 ttaaattccg catttaatgt acctgataat aaagttcttt tttatgcaac cgacagcacc
     4321 aacagtatcc aacaaattga ggaacttata actagtagcg gtttcatacc atttaacgtt
     4381 ggcggtatag accaaagtat caaaatagaa gtatttggcg acctgcacga atttggagcg
     4441 ttgggcaaac cggtaacact tgctgaagcg aaaaacaaaa ttgctcaccc ttaaaactaa
     4501 caatatgaaa aatttaatca tttccgcaat gttgcttgta gtattagcat cttgccaaaa
     4561 cgaaacaaaa aatcaatcaa acaattcaac acaaaaaact atggaacagt ctaccgaaaa
     4621 attagcaatt gaaaaattac tattctctta tcgtgatgct ttaaacagct cagatgtaaa
     4681 taaagtgttg ccactttata caaacgatgg tgtttttatg ccatcaaatg caccatcagc
     4741 aatcggccag gaacaggtaa aagtatctta tgaattcgtt ttttcgcaaa ttcaactaaa
     4801 tattgaattt tacattgatg aaatagtggt aaacggcgac tatgcttttg ctcggacaac
     4861 ctcaaaagga aagactttaa ttcgcgctaa cggagaaact gtgccagaag aaaacagaga
     4921 actttttgtt ctgcaaaaaa caaacggaca atggaaaata agccgttata tgtttaacaa
     4981 aatgaaatag aaggactaaa agggataatt aaaacagcaa ttttgcgtca tacggagttt
     5041 tttgcttcgt ttgacgcttt tgcgtatatt tgaatacggt tgttcatttc aattgatatc
     5101 ataaaatctc gcctgaatgc aaatcatata acattagtaa caaaatggtg ggacatctca
     5161 aacgataaaa gtctaaaata tgaaatgtat gtttccttta ctttttctgt tattcttttc
     5221 gtgccaaaac actactaatg aaaacaaagt atcagatgta aaaacgaagg aagttgtttc
     5281 agatacagtt tacacttgga caaaattgtt agacagcgct gcgtggaaaa agaattacaa
     5341 tttccaaatg ttcagtatta atgacacttt atggacattt cacccggata gaatttggta
     5401 ctcaaaaaac gggaaaaatt ggacaaaatc ggttcttgaa aattcaatta ataatcttgc
     5461 gtttcttgac tatgtttatt tcaaaggttc agtttacgga cttggatatt tcagcggtaa
     5521 tattgaacaa ttcacattta aaccggaaat agtaaagaca acagacttta aaagttggac
     5581 gaccatctct aggacaagca acttgccaaa tcgttttttt tatcatccat ttgtttttga
     5641 aaataaaatt tggattgttg gtggagagga taaaaacaca aaatacgccg acatttggaa
     5701 ttcagaagat ggtattacat ggactaaaca aaaagaaaat cttccctttg gtaaaagaag
     5761 tggaagtcaa attgttacac ttaacaataa cttgtttttg cttgataatg atgtttggcg
     5821 ttcaatagat ggtttgaatt gggaaaaagt agctgacgag attgtaaaag gcgaacaaat
     5881 atttggatac gctgctcaag tatttgataa taaaatttgg ctacttggtt gtaacagaaa
     5941 tggacagttt tctagccaag ttctttatag ttcagacggg aaaaattggc aaacaaatac
     6001 tgctccctgg ctgccccgtg gtggaattgc agcaactgtt cacaacaaca aaatatatat
     6061 gacaggcgga aaatacggcg gcacacccaa ccacccggaa ttcagatacg ataatgatgt
     6121 ttggacactt gaaaaaaggg aataaaagaa aaacagtcac tactaacgaa ttggcaattt
     6181 gtccaaatgc tggggtgata ttttacaaat taaccaacat cgtcaagtcg taaaacgtca
     6241 tgagcattta atatttacat cgagttaaaa attcaattac ctaacaggaa agtaatttaa
     6301 tagttgtctc gatgatactt gctaaagtcc acttcaaaat atcagaggaa gtgatttaac
     6361 tgtagtcgat tgccggaagg caggtttttc tgcgctaaaa ttacttccga caataaatga
     6421 tggtcgaata aaaagaaaat accattacat attatacgtt ttttcgaaag cgggctattc
     6481 tatatacctg taattttgtt cttaaatgca ccgaagaaaa taattaaaac gttttaccat
     6541 gtaaaacgac tgaacctaaa atcgaaaaaa aatgcaacac cataaaaatt atattatgat
     6601 gttgcattaa agtagaattt aatccttcaa ttgatttacc tcaacttctc taccctacct
     6661 aaataaacag ttttaccaac aattaattta accacataaa tcccggtagg taatactgat
     6721 atatttacat tcaaagcgtt ttcatttatg atttgagttt caaatggtga aattatctgt
     6781 ttaccctgta gatcatacat ttccaaaact atttcattcc cacttaaatt ttccagctct
     6841 acaataaaaa attcatctgc agggtttgga aataagttaa tagagcgaat atcagtattt
     6901 gaaataggcg taacttgaat agtaattaac atattatatt cagaaattcc acaatcattt
     6961 tctacaatta gtgttaccga ataattacct tcagttgcat aagtatgtgt agggtctatt
     7021 tcggtactac catctccgtc accaaaaacc cagaaatatg tcccggttcc tgaggaagtg
     7081 ttttcaaaaa atacagtaag ccctgatgaa gtgtaattaa aagaagcttc cggtgcaaat
     7141 tcaagggtga cattaacggt atcgctaatc tcacaacctg ataccgggtc acttatacta
     7201 acagagtagg ttccggaact tgtaaccgtt atagtttgtg tgatttctcc tgtgttccat
     7261 acatattcag tacccggatt tcctgcatca agaattactg catcacatgc cgttatattt
     7321 tctggtaaca tcgctatagg tgaggcataa gcattaatgc ttaacgtaaa agtatcgttg
     7381 ctaatatcaa tatcatccgg aagcagggtg tatatttcaa aaacatattc acccggaaca
     7441 gaaagatcgg cagtagttgc aaaagtgtat tcagcttcaa acccgggtgc aatggaaacg
     7501 tcagctattt ccataatttc cggaccacca tttattttat acgctacagg aaaagcactt
     7561 attgttgttc caccgtaatt ttgaatagta atttttattt cctccgtttc agatttatta
     7621 caggaagaaa ctaattccgg taaattgatg acacctgcat catcaggaat aactacatta
     7681 caatcaccta aatacaccaa aatgttttga cgcagattca tatttttatc cgccggatca
     7741 attaactgac ttggtgccca tgcactcata attactttgc caattcccca ttgtttttct
     7801 atcatacagg gaatatcatt atgtggtgca ccaaacaaca cgccatcaat attttcatgt
     7861 gcaagtgtat cataattctt tccatatatc accgcattac tcgcataaaa gccactgaaa
     7921 gttgtgccaa ccggcgtata tggtccttca taaacagggt gtgtaattcc ggttttcttt
     7981 ttaatatccg taacctgata actcatcgca atttttgttc cgtcgaatcc gtaatccaca
     8041 taaaaatgct ccccttcact ttctgaccca ctgctgttca aatataattt tccgccggct
     8101 gctacccagt tttccattaa ttgacggtta tcatttaaga ataattccat cgcaactata
     8161 tgattataac ttccatctaa aaaaataaaa catgatgaat cggaaaataa tatttcaggg
     8221 tcaacagttt caaaatattc ctgcgtccag ttgccctctc caaaaacagt attcatacag
     8281 gttgtattcg tagtattaaa atatggtgcg ccactaagtg cttctgcacc tccatcaata
     8341 ttggaatgta tcaaccattt tttagtaccg ctaacataat tcaaattaca gatatttgct
     8401 tccaggttat catttaacgg aaatccatca tccggatgtt gtacttccag tgtgatggca
     8461 taacatccta aaccggcaag gttagccggt tccgaaaaag tataaataaa atcgctgttg
     8521 gcgggaatta catcaggaac tgtttctacc acctcaggac caccgtcaat ggaataagca
     8581 acatttacat ctgaaaaatc tgtatcggta tgattggtaa tagcaatggt aatactctct
     8641 tcagttaatt caaccgctga aacaggagat attatttttt ttatactgag atcatgttca
     8701 atgccttcgg aaattaggac agtataatct tcagtatcac cttgtaaaaa tggcagacaa
     8761 gggtcgttag aattcatcca ggggccaaat ggttcgtgaa cagcacggac tctcattcta
     8821 gtttctccat tgatggcatc ggcaggaata ctaaaagtga tgctccccga ctgaaccccg
     8881 gttaaaacga tagttcccaa tttttctgct tcttcaaatt ccatattctg attaaaatca
     8941 atccaaaccg aataattata ctgccaggat ttattgtcga gataaatggt attggtggac
     9001 ccggcaataa gcgctgtgga gagttcagta taatcgctgt aaccgatatt ttcttcagca
     9061 accccgcttc cggtattcat aatgtcgcca agttccactc cgtcgatgat tacatcgagc
     9121 atgggaaacg aatgttgagg aatacaatat tgtgctgaaa tgttgagtgt gaatatttgt
     9181 aaacaaataa aaattatcca tgtggaagga cgaagtttgt ttaaattttt catatgcatt
     9241 ttttaatgca aatcttctcc ataccattat taatagcgtc ctgaattata aattcagacc
     9301 tgatttattt gttcatgact taattatgac aaaataactg ttagcgcatg ttaaagcaag
     9361 ttaaaattgg tagggtcatt gtttattatt atttttattt atcaaaattt acatcatgtt
     9421 acctgaacca tcgtttacca ttgggtcttt tgtatttctg attatttcgg ctcagggatt
     9481 atttcttgcg gtattgctcg ccgtatacaa taggtcaaat gtagttgcaa acagaactat
     9541 ggcaattttt gttgctgcat tttcgatgat cttgtgttat tatgtgttat actggacagg
     9601 ttacagttgc aatttcatgt ggaccaaggg ttggtgttcg gccttgccgt ttttgttggg
     9661 gcctttgatg tatttatata ttattatcat aaaaaacgga gagcttccaa caaactataa
     9721 aaaacacttt attccctttg cggtgcacct cacctatatg ttgccattta tatattggtt
     9781 cactacaggg cctgaggtaa ttgcgacatc gcaagaaccc atgaatcttt ttttgcaaga
     9841 taagatatat cgtcacctga attttgtaat tacatcgttt caggtattca atgccctgca
     9901 gtttttgagt atgtttattt atgccttttt attattgcgt ttattaatca aacaacaaaa
     9961 ggaaaaacat ggcagcgaac atcaacaaat gaaatatcgt tggtatgttc gaaatatttc
    10021 cttttatgta ttgttctccc tttgtttcag cagttattgg ataacacttt attttggaat
    10081 ttccatcgat ggattggatt atttagtatc cggaataatg tcactttgta tctacctgat
    10141 tggatatggt gctttccgtc agcccttatc gttatttgaa caaaagaatg ccattaccag
    10201 aaaaaaactc cctcccgcag atttaatact tcaaagtcaa aaactgacta catacctgga
    10261 aacacaaaag ccctggctcg actctgaatt aaatttgaac aaactgtccg agttattaca
    10321 aatgcctgtg cacgaattat cctttttaat taattccacc ctacataaaa atttctctga
    10381 actcgtgcac gattatcgca tcaaggaagc ctgccgactt cttctcgacg agtcctattc
    10441 cgactcgaaa attctcagta ttgcattcga cgtaggctac actaataaag caacctttaa
    10501 tgccgctttc aaaaacaaaa tgggtgtttc gccaagcgct tataaattaa tggcgaaggg
    10561 acatgggatg aatgaggcta taaatctgtg aaggattttt aatttttatt ggagtttata
    10621 gaatgttttg gtataatcaa tgaattacaa agaataggta ttcataacac gaagctaaca
    10681 acaatcataa ataaaggctt cagaaacgcc actaatttca gacattcgaa ttcaagaagt
    10741 att
//
LOCUS       DAORKW010000057        10658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_125_length_10658_cov_5.038857, whole genome shotgun sequence.
ACCESSION   DAORKW010000057 DAORKW010000000
VERSION     DAORKW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125_length_10658_cov_5.038857"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PLB46_03945"
     CDS             <1..882
                     /locus_tag="PLB46_03945"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HRF75700.1"
                     /translation="IIPVNKFIYLSHNLILMQHTTYHKQNKKGQEIFAQSWTGEKEPI
                     AVICHIHGQSDHSSRFEHVAAFFVAHQVDFFAADLIGHGKSGGARGHVNHFDEYLETV
                     DMLYDEVVTKYPGKPIFIYGHSMGGNVVINHALQNTKPVTGYIATSPWIRLAFDPPAW
                     KVALGKTVKSIFPALLQPTGLNPALISHDKEVVEKYKNDKLVHGKISAAGFFEIFTKG
                     KSILERSSELHYPMFIAHGTEDQLTDHTASKAFAQMRSDLIEYKEFPGLFHEMHNEPE
                     KEQLFNAILHFIHQKIQ"
     gene            884..1342
                     /locus_tag="PLB46_03950"
     CDS             884..1342
                     /locus_tag="PLB46_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75701.1"
                     /translation="MSTQTLGYLKTLQIMFMGLFTGPILFLGVCFMLQAGNQPVEQEM
                     SSPLFFIAIGFATIGIIGSRAMYASRISKLQALESTQQKLDGWRTTFIIRMAMIEGPT
                     LLCVVGLLIEEQRVFLFLALVLLFFQALNFPTKSKIQSDLNLSETETKEL"
     gene            complement(1540..2598)
                     /locus_tag="PLB46_03955"
     CDS             complement(1540..2598)
                     /locus_tag="PLB46_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF75702.1"
                     /translation="MKPIISGIQQVGIGNPFVHEAWRWYREKFGMDVKIFEEAAEANL
                     MLPYTGGKPHKRHAILAVNMNGGGGFEIWQYTSRKPEAATFEIQYGDLGIYACKMKSR
                     DVKATYEFLKKQNVNILGDISKNPNGDDHFYLRDLYGNVFEIVKAEDWFGKTNFTTGG
                     SYGAVIGVTDMEKSIAFYSDILGFDKVIYDKEEIFSDTAVLTGGNNKSRRVLLAQSES
                     RRGPFSKLLGNGEIELVKVYDRTPNKIFANRYWGDLGFIHLCFDIQGMDKMKSLCESK
                     GYPFTVDSGNHFDMGEAAGHFSYIEDPDGALIEFVETHKVPILKKFGWYLNLKKRDPN
                     EALPNWMVKAMGLNRVKG"
     gene            complement(2627..3424)
                     /locus_tag="PLB46_03960"
     CDS             complement(2627..3424)
                     /locus_tag="PLB46_03960"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF75703.1"
                     /translation="MFAEKVIWITGASSGIGEELAKQLAKEKAYLILSARRIEELERV
                     KQYCLQFTNKVEILPVDLSLNHTLNDAANKALSFFGKIDMLVNNGGISQRSGVLETSE
                     EVEQRIMQINFTSAVTLTKAVLPQMEKNKFGNIILMSSITGKIGVPNRSTYCASKHAI
                     IGYFDALRAELKIRKSPVNIHIIMPGYVHTNISLHAVQGDGKAQGLMDKGQANGMPVE
                     DCVRKILSAVRRNKKEVLIGRKEILMAHFRRFIPSLYYHLIAKVATK"
     gene            complement(3463..4167)
                     /locus_tag="PLB46_03965"
     CDS             complement(3463..4167)
                     /locus_tag="PLB46_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRF75704.1"
                     /translation="MRYSIIIPTLNEERFIHRLISHVQLAGEGESYEIIVCDGGSSDR
                     TVSVAAGLGVRTYESPKKGRAGQMNFAAARAKGEILYFVHADCLPPITFFKEIENQLK
                     KGADAGCFRYKFDSDKFMLRVNAFFNRFGGLICRGGDQTLFIKRTIFNSLGGFNEKFT
                     IMEDYDLVCSIRKQYRFAVIPAYAVVSSRKYEHNNWLQVNFANSIAMFMFISGACSPD
                     RIKSTYHSLIRHPKDE"
     gene            complement(4171..4704)
                     /locus_tag="PLB46_03970"
     CDS             complement(4171..4704)
                     /locus_tag="PLB46_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF75705.1"
                     /translation="MEANLFEMEVIIRKANPDDSKSLAELGAKTFFDTYHNDNTPEDM
                     ADYITKYFNINVLQVELLDKNTLFFVAETNQELIGYIKLNLIGNSYIPNEKAMEVSRF
                     YVDKQFQGHKLGAKLMLEAEKYAIECGCEVMWLSVWQKNLTSITIYEKLGYNKIGTTT
                     FKLGKDIQDDYIMVKHL"
     gene            4888..5268
                     /locus_tag="PLB46_03975"
     CDS             4888..5268
                     /locus_tag="PLB46_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75706.1"
                     /translation="MKKLITLTLVLLTGISVTFACAGPEGEDLIRKAATIVDETAQQQ
                     YCKPEGKKEISYASVMPAADEKNVVITFTIDEQNKIHIVEVQGGYAYINHYIKTSLEG
                     KEIKTENAIPGINYIMTVKLPTSV"
     gene            5547..8027
                     /locus_tag="PLB46_03980"
     CDS             5547..8027
                     /locus_tag="PLB46_03980"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRF75707.1"
                     /translation="MIRNVFTFILAFTAFTLQAQQLQWNSSRILLEIEKLNSTASVLY
                     IAAHPDDENTRLITYLANEKRVRTGYLSLTRGDGGQNLIGEEQGAYLGIIRTQELLAA
                     RRLDGGEQFFTRAVDFGYSKTAAETFTKWPKDTILSDMVWVIRNFKPDIIITRFPADE
                     RAGHGHHTASAMLAEEAFAAAADPTKFPEQLQYVSVWQAQRLFWNNSTWWDKDLPNKI
                     ANGAPELAVFDVGGYNTLLGKSYGEIAAESRTNHKSQGFGSTPTRGEQKEYLELKKGT
                     AIQNSDIFSGIQLGWERYRQGADIQNTINNIISNYDILHPEKSVDALLKVYGQLKLIP
                     EDQLIQYKKQQVQKIIVACLGLWLEPVAEKDMVANGDNLNIVSTSLKRVDYPLSLESI
                     TVLGVEYKSGEILPAGINQIDSFKIVVPEGISSSPYWLNGAYDGVFNVEDRKLIGKAE
                     NDPAIIFTYNIKVSTEVFAINRGVVYKETYAVKGEIYTPLAVVPRAIVHADSYNLIYT
                     DSLSKSFPLLIKSYAADTIAGNVRLFGNRANISAKPYTLNKNGESSTLNFTITPPKQN
                     GTKEIGLNISGNNENETLGESAKKIRVISYDHIPRQVVVEDAKLHVVKFDAMPSTKKV
                     LYIDGAGDLVDESLKQIGVNITTIKPEDMNAALLQQYDVAIIGVRAFNTSKVLADNNA
                     QLLEFVNAGGTLIAQYSTNWDMYLEKIGPYNFKITRTRVTDENSPVEFLLPEHKVLNT
                     PNKITQDDFTGWVQERGIYFAGELAPEYVAPLAFTDPNEQPQNGSLIIAQYGKGTFMY
                     TGLAFFRELPAGVAGAYRLMLNMIEYKP"
     gene            8146..9870
                     /locus_tag="PLB46_03985"
     CDS             8146..9870
                     /locus_tag="PLB46_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRF75708.1"
                     /translation="MSTIDWLVMFGSLLGIAMFGIYKTRSVHTAQSYLQGDNEKKWWE
                     IGLSVMATQASAITFLSVPGQAYDDGLRFIQFYFGLPLAMIVIAVFFIPMYYKLKVYT
                     AYEFLEQRFDVKTRTFTALLFLIQRAFSTGITIYAPSIILSQLLGWDLNTTVIFIGGI
                     VTLYILSGGTKAVAVTHKQQMFVIYVGMFTAFGCLIYYLSEYVSFNESLNVAGALGKM
                     NIVTTGAESGSFDWSDRYNIISGLIGGLFLQLSYFGTDQSQVGRYLGGESVKESRLGL
                     IFNGLMKVPIQFFILLTGVLVFVFYQFNQPPVYFNEVAYTQTKNSIYAEELVSLETAY
                     NAATANQTKLNRELAEQKITATPHQTELLQNSQKQVNGLRTEINAVIKKANPDIDDDH
                     DYIFMHFVMNYLPIGLVGLLFAVIFSASMSSTSSALNGLATTFSVDIYKRFINKKASE
                     QQHLNASKVLTVLFGAMAIGFALICSMFDNLIEAVNILGSLFYGTILGIFLTAFLLKR
                     VKGNAVFIAALLSEAVILYLDFSVRYNWDTPQLAIGYLWYNVIGCLLVMLIASVLSLG
                     NKRPTTAM"
     gene            complement(9925..10332)
                     /locus_tag="PLB46_03990"
     CDS             complement(9925..10332)
                     /locus_tag="PLB46_03990"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HRF75709.1"
                     /translation="MISNLLDNLNWLAALAGGAAYYVIGAAWYGILGKGWMEAAGLTS
                     EQIKTNFNKAVYGLTLVVEIIIVAFMGGFMSAETSISDAAQLGLVVGLIFSGLTTYVH
                     YLYTMRKPNLIFYDAGYTTIASIVAAVVYAAIN"
     gene            10492..>10658
                     /locus_tag="PLB46_03995"
     CDS             10492..>10658
                     /locus_tag="PLB46_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRF75710.1"
                     /translation="MQQYLDLLQKILDEGHQKSDRTGTGTISLFGYQMRFNLQDGFPL
                     LTTKKLHTRSI"
BASE COUNT         3344 a         1948 c         1968 g         3398 t
ORIGIN      
        1 ataatacctg taaataaatt tatatattta tcacataatt taatattgat gcaacatact
       61 acttaccata aacaaaataa aaaaggccag gaaattttcg cccaaagctg gaccggtgaa
      121 aaggagccaa ttgctgtgat atgccatatt cacggccaaa gcgaccacag ctcaagattt
      181 gagcacgtag cggccttttt tgtggcgcat caggtagact tttttgctgc cgatttgatt
      241 ggtcacggga agtcaggtgg tgccagagga catgtcaatc actttgatga atatctggaa
      301 acagtagata tgttatacga tgaggtagtt acaaaatacc ccggaaaacc gattttcatt
      361 tatggccata gtatgggtgg taatgtggta atcaaccatg cattacaaaa cacaaaaccg
      421 gtgacaggat atattgctac atcgccatgg atacgattag cattcgaccc accggcatgg
      481 aaagtagcct tgggcaaaac agtgaagtca atattccctg ctttgttaca accgacaggt
      541 ttaaatcctg cactaatttc ccacgacaaa gaagtagtag aaaaatataa aaatgataaa
      601 ctggtgcatg gtaaaatttc agcggcagga tttttcgaaa tttttacaaa aggaaaatca
      661 atattggaaa gatcatctga attgcattat cctatgttta ttgcacatgg cactgaggac
      721 caactgactg atcatactgc aagtaaagca tttgcccaaa tgcgcagcga tttaattgag
      781 tataaagaat ttcctggttt atttcatgaa atgcataatg aacctgaaaa ggaacaatta
      841 tttaatgcta ttttacactt cattcaccaa aaaattcaat aagatgagca cacaaacgct
      901 gggttattta aaaacactac aaatcatgtt tatgggatta tttaccgggc ccatactttt
      961 tttgggagtg tgttttatgt tacaagcagg taatcaaccg gttgagcaag aaatgagcag
     1021 tccattattt tttatcgcaa ttggatttgc aaccattggt ataattggtt caagagctat
     1081 gtatgcatca cgtataagca agttgcaagc acttgaaagt acacaacaaa aattagatgg
     1141 ctggagaact acatttatta tcagaatggc catgattgag gggccaactt tattatgtgt
     1201 tgtgggattg ttaattgaag aacaacgtgt gtttttattt ttagccttgg tattattatt
     1261 ttttcaggcg ctcaatttcc caacaaaatc aaaaatccaa tccgacctca atttaagtga
     1321 aactgaaacg aaggaattat aacattaaat taaaaaggag agacaccata tttcaataat
     1381 tccatttaat tatttcaaca aatcattaaa tccacaacaa caaaaatcac ccaatccaat
     1441 ttcaatattg ctctcccaac agctattgag actgttttaa ttttgcttct cccgatagct
     1501 atcgggattg ctcaatcttg attcaatcct gttcaatccc taccccttca ccctattcaa
     1561 ccccatcgct ttcaccatcc aattcggtaa agcctcattc gggtcgcgtt ttttgagatt
     1621 taaataccag ccgaattttt tcaagatagg cactttatgc gtttctacaa attcaattaa
     1681 tgcaccatca ggatcttcga tataactaaa atgtcctgcc gcttcaccca tgtcgaaatg
     1741 gttgccacta tctacagtaa acggatatcc ttttgattca cacaaagatt tcattttatc
     1801 catgccctga atgtcgaagc ataaatgaat aaatcctaaa tcaccccaat aacgattggc
     1861 aaatatttta tttggtgtgc ggtcgtagac ttttaccaat tcaatttctc cgttgcctaa
     1921 taatttacta aaaggaccac gacgtgattc agattgtgct aataatactc tacgagattt
     1981 attatttcct cctgttaata ccgcagtgtc gctaaatatt tcttctttat cgtaaataac
     2041 cttatcgaaa cctaatatgt cgctataaaa cgcaattgat ttttccatat cagttacacc
     2101 aatcaccgca ccataactac cacctgtagt gaaatttgtt ttgccaaacc aatcctcagc
     2161 ttttacaatt tcaaatacat tgccatacaa gtcgcgtaaa taaaagtgat catcgccatt
     2221 tggattttta ctgatatcgc ctaaaatatt tacattttgt tttttgagaa attcgtaagt
     2281 ggctttaacg tcgcgacttt tcatcttaca cgcataaatg cccaaatcgc catattgaat
     2341 ttcgaaagtc gctgcttctg gtttgcgtga agtgtattgc caaatttcaa atccaccacc
     2401 accgttcata tttacagcta atatcgcgtg acgcttatgt ggtttacctc ctgtgtatgg
     2461 taacattaaa tttgcttctg cagcctcttc aaaaatcttt acatccattc cgaatttttc
     2521 tcggtaccat ctccatgctt cgtgaacaaa cggattacct attcctacct gctgaatgcc
     2581 actaataatt ggtttcattt tgctttatac aatttatttt aataatttat ttggttgcca
     2641 ctttggcgat taagtgataa tacaatgagg gaataaatct tctgaaatgc gccatcaaaa
     2701 tttctttacg gccaattaat acttcttttt tatttcggcg aaccgcactt aatattttcc
     2761 gtacacaatc ttcaaccggc atgccattag cctggccttt gtccatcaat ccttgtgctt
     2821 ttccatcgcc ttgcacagcg tgtaacgata tattggtatg tacatatccc ggcataataa
     2881 tatgaatatt aaccggtgat tttctaattt ttaattctgc acgtaaagca tcaaaataac
     2941 caataatggc atgtttactt gcacaataag ttgaacgatt tggaacacca attttacctg
     3001 tgatactgct catgagaata atattgccaa atttgttttt ttccatttgt ggtaaaacag
     3061 ctttggttaa agtgacagca ctggtgaaat taatttgcat aatccgttgc tccacttctt
     3121 cactggtttc taaaacgccg gagcgctgac taattcctcc gttatttacc agcatatcta
     3181 ttttgccgaa gaagcttagt gctttattgg cagcatcgtt taaggtatgg ttaagcgata
     3241 aatcaacagg taatatttca accttgttgg tgaattgcag acagtattgt ttcacccttt
     3301 ccaattcttc aattcggcgg gctgataaaa tgagatatgc tttttctttt gcgagttgtt
     3361 tcgccagttc ttcgcctatg cctgacgaag ctccggttat ccaaataact ttttctgcaa
     3421 acatgggtgc aaagataccc aaagaattgt ttgagttaag agttactcat cttttgggtg
     3481 cctgatgaga ctgtggtaag tggatttaat cctgtcggga gagcaggctc cggagataaa
     3541 cataaacatg gcaatgctgt tagcgaaatt tacctgaagc cagttattgt gttcgtattt
     3601 gcgggatgat accacggcat aagcaggtat tacagcaaag cggtattgct tgcgaatact
     3661 gcacaccaga tcgtaatcct ccataatggt aaatttttcg ttgaaacctc ccaaactgtt
     3721 gaaaatagtg cgttttatga acagggtttg atcgccacct ctacaaatta atccgccaaa
     3781 cctgttaaaa aaggcattca ccctaagcat gaacttatca gaatcgaatt tatacctgaa
     3841 acagccggca tcggcacctt tttttaattg attttcaatt tctttaaaaa atgtaatcgg
     3901 tggtaaacaa tcggcatgga cgaaatataa aatttctcct ttagctctgg ctgctgcaaa
     3961 gttcatttgt ccggcgcggc cttttttagg tgactcataa gtcctaaccc ctaaacctgc
     4021 cgcaacgctt acggttcggt cactgcttcc accatcacaa acaattattt catacgattc
     4081 accctcgccg gctaattgca catgactgat gaggcgatga ataaatcgct cttcgtttaa
     4141 ggtcggtatg atgatagaat aacgcatatg ttataaatgc tttaccatga tatagtcatc
     4201 ctggatgtcc ttccccaatt taaatgttgt tgtaccaatc ttgttatagc ccaatttttc
     4261 ataaatggta atggaagtta agtttttttg ccaaaccgac aaccacataa cctcacaacc
     4321 acattctatt gcatattttt cagcttccaa catcaatttt gcccccaatt tatggccctg
     4381 aaattgttta tctacataaa acctggacac ttccattgct ttttcgttgg gtatatacga
     4441 attacctatg aggtttagtt ttatgtaacc aattaattcc tgatttgttt cagcgacaaa
     4501 aaataaggtg tttttatcca gtaattcaac ctgtaataca ttgatattga aatacttagt
     4561 aatataatcg gccatatcct caggggtatt gtcattatga taggtatcaa aaaatgtttt
     4621 tgcccccaat tcggctaagg atttggaatc gtccgggttt gcttttctga ttattacttc
     4681 catctcaaat aaattcgcct ccaattttac caattgatag acgaaattta ggcatttgtc
     4741 gaaaaaagat gttaaaattt gtcacccacc tgtaacttta ggcatagttc atgcgtcaaa
     4801 gcgttaataa tggttaataa cctaactaat taacttaaac tgttttaatt tcacagtgca
     4861 gcaatgcacc tttgtaaaac ccggattatg aaaaagctga taaccttaac ccttgtgcta
     4921 ttaaccggaa tttcggtaac gtttgcctgc gctggaccag aaggtgaaga ccttattaga
     4981 aaagcagcaa caatagttga cgaaactgcc cagcaacaat attgcaaacc ggaaggtaaa
     5041 aaggagattt cctatgcttc agttatgccg gctgcagatg aaaaaaatgt ggttatcaca
     5101 tttacaatcg acgaacagaa taaaattcat atagtggaag tgcagggtgg ttatgcctat
     5161 attaatcact atatcaaaac ttcattagaa gggaaagaaa taaaaaccga aaatgccatt
     5221 cccggaataa actatataat gacagttaaa ttgccaactt cagtttaagc gcaattcaat
     5281 aatatgttaa aacctccgat aatcgggggt tttttgtttt ccggatattg tggattccct
     5341 tttagtgcaa agcttggctg cgtttacaaa gatgttttac gtatcaacca tgatgaaatg
     5401 ctaactgtgt aactttaagg ctcataactt ttgcctacat ttgtttctta aacattaatc
     5461 aggcatacgg ttagttacaa tgggaacaat tcatattgag ccggtgttac tggtttttta
     5521 ccaatcagtt ttattatagc aattttatga ttcgaaatgt ttttactttt attttagcat
     5581 ttactgcttt tacacttcaa gcacaacaac tacagtggaa ttcttcccga attttattgg
     5641 aaatagaaaa attaaattca acagcaagtg tattatatat agcagcacac cctgatgatg
     5701 aaaatacgcg tttaataact tacctggcta atgaaaaacg tgtgcgcacc ggatacttat
     5761 cacttacacg tggcgatggt ggtcaaaatc tgattggtga agagcaaggc gcttatttag
     5821 gcataattcg tacacaggaa ttattggcag ctcgtcgttt agatggaggc gaacaatttt
     5881 ttactcgcgc tgtagatttt ggttacagca aaacggctgc agaaacattt actaaatggc
     5941 caaaggatac catattatcc gacatggttt gggtaattcg taattttaaa ccggatatta
     6001 ttattacccg atttcctgca gatgaaagag ccggtcatgg tcaccacaca gcaagtgcaa
     6061 tgttagctga agaagctttt gccgcagctg ctgacccaac taaatttcct gaacaattac
     6121 aatatgtaag tgtatggcaa gcacaacgtt tattttggaa taactcaacc tggtgggata
     6181 aagatttacc caataaaatt gccaatggcg cacctgaatt agctgttttt gatgtgggag
     6241 gatataatac tttgttagga aaaagttatg gagaaattgc agcagaaagt cgcaccaatc
     6301 ataaaagtca gggatttggc agcacaccaa cccgcggcga acaaaaagaa taccttgaat
     6361 taaaaaaagg aactgccatt caaaatagcg atattttttc aggcatacaa ttaggttggg
     6421 agcgctatcg tcaaggggca gatattcaga atacgataaa taatattatc agcaactacg
     6481 atattttaca tcctgaaaaa tcagtagatg cattacttaa agtttacggg caattaaaat
     6541 taattcctga agatcaactc attcaatata aaaaacaaca agtccaaaaa ataattgttg
     6601 cctgtttagg attatggcta gagccggttg cagaaaaaga tatggttgcg aatggcgata
     6661 atttaaatat tgttagtact tcgttgaagc gtgttgatta tccattgtca ttggaatcaa
     6721 taactgtttt aggtgttgaa tataaatcag gtgaaatttt gcctgcagga ataaatcaaa
     6781 tcgattcatt taaaatagtt gtacctgaag gtatttcttc atcaccgtat tggttaaatg
     6841 gtgcttatga tggcgtattt aatgtagagg acagaaaatt aataggcaaa gcagaaaatg
     6901 acccggcaat tattttcacg tataatataa aagtcagcac cgaagttttt gcgattaaca
     6961 ggggagtagt ttataaagaa acgtatgcgg ttaaaggcga aatatatacg ccattagcag
     7021 tagttccaag ggcaatagtg cacgcagata gttataattt aatttatact gatagtttga
     7081 gcaaaagttt tccattatta attaaatcat atgcagcaga tactattgca ggtaatgtgc
     7141 gtttatttgg gaatagggca aatattagtg ctaaacccta tacattaaat aaaaatggag
     7201 aatcttccac gctcaatttc actatcactc cgccaaaaca aaatggcaca aaagaaattg
     7261 gattaaatat ttcgggaaat aatgaaaacg aaacattagg agaaagcgca aaaaaaatcc
     7321 gagttataag ttacgaccat atccctcgtc aggttgtggt ggaagatgct aaattgcatg
     7381 tggtgaaatt tgatgcaatg ccttctacta aaaaagtact ttacattgat ggtgccggcg
     7441 acttggtgga tgaaagtctc aaacaaattg gtgtaaatat tacaactatt aaacctgaag
     7501 atatgaatgc cgccttgctg caacaatacg atgtggctat aattggcgtg cgcgcattta
     7561 atacctcaaa agtattagct gataataatg cacaattgtt agaatttgta aatgcaggtg
     7621 gtactttaat tgcacaatat tctaccaatt gggatatgta tctggaaaaa atagggccat
     7681 ataattttaa aattacccga accagagtta ccgatgaaaa ttcacctgtt gaatttttat
     7741 tacctgaaca taaagtactt aatactccga ataaaattac acaagacgat tttactggat
     7801 gggtgcagga acgaggaata tattttgcag gagaattagc gcctgaatac gtggctcctt
     7861 tggcatttac tgaccccaat gaacaacctc aaaacggcag tttaattatt gctcaatatg
     7921 gtaaaggaac attcatgtat accggacttg cattttttag agaacttcct gcaggtgttg
     7981 caggcgcgta tagattaatg ttgaatatga ttgaatacaa accttaatta ttatttacat
     8041 gcctgaaaat aaaccgtcaa attattggac aaactggtat gtaattgttg cagtactctt
     8101 gttgctggag attattattt tcatagcatt aacccataaa tacgcatgag tactatcgat
     8161 tggttggtaa tgtttggcag tttattgggg attgccatgt ttggcattta caaaaccagg
     8221 agcgtacaca ccgcacaaag ttatttacag ggtgataatg aaaaaaagtg gtgggaaatc
     8281 ggactaagcg taatggctac tcaggccagt gctatcactt ttttatcggt gccgggtcag
     8341 gcttacgatg atggcttgcg ttttattcag ttttattttg gtttgccttt ggcgatgata
     8401 gtaattgctg tgttttttat tcctatgtac tacaaattaa aagtgtacac agcatatgaa
     8461 tttttagaac aacgatttga tgtaaaaaca agaacattta cagcattatt atttttaatt
     8521 caacgtgcat tttctacggg tataactatt tatgcgcctt caataatttt atcgcaatta
     8581 ttgggttggg atttaaatac gactgtaata tttattggag gaattgtaac cttatacatt
     8641 ttatcaggag ggactaaggc tgttgccgtg acgcacaagc aacaaatgtt tgtgatttat
     8701 gttggtatgt ttaccgcttt tggttgcctt atttattacc tcagtgaata tgtatctttt
     8761 aacgaatcac tgaatgttgc cggcgctttg gggaaaatga atatcgttac aaccggagct
     8821 gaatccggca gttttgactg gagtgaccga tataatatta tttcaggatt aattggggga
     8881 ctgtttttgc agttgagtta ttttgggaca gaccaaagtc aggtaggtcg ttacctcggt
     8941 ggtgaatcgg tgaaagaaag ccgattaggt ttgattttta acggtttaat gaaagtccct
     9001 attcagtttt ttattttgtt aaccggtgta ttagtttttg tgttttatca gtttaatcaa
     9061 ccaccggttt attttaatga agttgcctat acacaaacaa aaaattccat ttatgctgaa
     9121 gagttggttt cactggaaac agcctacaat gcagccactg ccaatcaaac aaaattaaac
     9181 cgggaactgg cggaacaaaa aataactgca accccacatc aaacagaatt gttacagaat
     9241 tcgcaaaagc aggtaaatgg gttacggaca gaaattaatg cggtaattaa aaaagcaaat
     9301 cccgatattg atgacgacca cgattatatt ttcatgcatt ttgttatgaa ttatctccca
     9361 attggattgg tcggtttatt gtttgcagtt atattttctg ctagtatgag ttcaacatca
     9421 tcagcattaa acggattggc cacaactttt tctgtagata tttataaacg ttttatcaat
     9481 aaaaaagcca gtgagcagca acatttaaac gcaagtaaag tgctaactgt tttatttggc
     9541 gctatggcaa taggttttgc acttatttgt tcaatgtttg acaacctgat tgaagctgta
     9601 aatattttag gcagtttatt ttacggaact attttaggta tttttttaac ggcattttta
     9661 ttgaaacggg taaaagggaa tgctgttttt attgccgctt tattatcaga ggctgtaata
     9721 ttgtatttgg attttagtgt gcgttataat tgggatacac ctcaattagc gattgggtat
     9781 ttatggtata atgttatcgg atgtcttttg gtaatgttga tagcttcagt tttaagtttg
     9841 ggaaacaaaa ggccaactac ggcaatgtga aataaaaaaa cgacacacta ggtatgtcgt
     9901 ttttcgaata acttctattt tttgttaatt tattgctgca taaaccaccg ctgcaactat
     9961 acttgctatg gtagtataac ctgcgtcata aaaaatgaga ttcggtttgc gcatggtgta
    10021 taaataatgc acataggttg ttaagccgga aaaaattaat cctacgacaa ggcctaattg
    10081 cgctgcatcg cttatggatg tttctgccga cataaatcca cccataaatg ccacaataat
    10141 aatttccact accagggtaa ggccataaac agccttgttg aagttggttt taatttgttc
    10201 tgacgtaagt ccggcggctt ccatccatcc tttacctagt atgccatacc aggcagcgcc
    10261 gattacataa tacgccgcgc cccctgcgag ggctgccagc cagttaaggt tgtcgagtaa
    10321 gttgctgatc ataagtaatt ggtttaaaga taaatatact ggttttccaa tagaactggc
    10381 tattttttac acactttaac atgccgaaaa gcccgtttaa ctcatggttt tgctggtttt
    10441 tcactttcgt attgggaact attaacaggg tattttgctt aattttacaa catgcagcag
    10501 tatctcgatt tactacaaaa aattcttgat gaaggccatc aaaaatctga tagaaccggc
    10561 acaggtacta ttagtttgtt tggttatcaa atgcgcttca accttcagga tggttttcct
    10621 ttgcttacca caaaaaaatt acatacccgg agtatcat
//
LOCUS       DAORKW010000058        10524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_127_length_10524_cov_5.558793, whole genome shotgun sequence.
ACCESSION   DAORKW010000058 DAORKW010000000
VERSION     DAORKW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127_length_10524_cov_5.558793"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /gene="cysC"
                     /locus_tag="PLB46_04000"
     CDS             complement(<1..447)
                     /gene="cysC"
                     /locus_tag="PLB46_04000"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRF75711.1"
                     /translation="MSPVQDNIHPIFDRLLQRGDKETMLKQRSKVIWLTGLSGSGKST
                     IAEAVEKILHERGYLTMLLDGDNIRTGINNNLTFSEPDRVENIRRIAEVSKLFLNCGI
                     ITLNSFVSPTNEIRTLAKNIIGETDFVEVYVNAPLEVCEARDVKGLY"
     gene            complement(829..1614)
                     /locus_tag="PLB46_04005"
     CDS             complement(829..1614)
                     /locus_tag="PLB46_04005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF75712.1"
                     /translation="MPTALITGASSGLGLEFAKIHAANGGDLILVARSGNKLEQLKQE
                     LTAKHNIQVNIIVKDLSIPDAAQQVYDEVKNKQLKVDYLINNAGFGDFGLFANTNWEK
                     EHQMMQLNMTALAHLTKLFVKDMVVARSGKILNIASTASFQPGPTMAIYYATKAFVYY
                     FSSALYNELKPYGVSVTSFHPGPTKTGFQDAADMHSSRLLKLMPVPDALPVAKKGYAA
                     MMKGKRTAIPGLINNVFQMSSKMMPWWFIIPSTRFLQGSEEVK"
     gene            complement(1950..3344)
                     /locus_tag="PLB46_04010"
     CDS             complement(1950..3344)
                     /locus_tag="PLB46_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRF75713.1"
                     /translation="MSGIRTFFILLKKALKGENIDATQGKIDTVIFLLAVPMIAEMLM
                     ESIFALVDIIFVSMISVEAVAVVGLTESTLTIIYSLGFGLSMGATALVARRVGEKDPK
                     GAAEAGMQAIYLGLGLATIISLIGMFYSRDILILMGASDGMVETGKGYIQWMLCGNFA
                     ILLLFLINGIFRGAGDAGIAMRSLMLANLINIILDPLFIFGIWIFPELGVKGAAVATT
                     IGRSVGVLYQLYYLVNGKSMIVLKGKIWQIKTHIIAQLMKLAAGTAGQFIIASASWIF
                     LMRIMSEFGENVLAGYTTAIRIVVFTILPAWGISNAAATLVGQNLGANLPERAEKSVW
                     RTGYFNMLFMGFVMVLFFAIPEQLIHIITRDEAVVKEGVLCLRILCLGYIAYGYEMVL
                     ANAINGAGDSKTPTIVNFFGFWILQIPLAYFLSTVLDLKSTGVYMAVPISEACMAIAF
                     VIIFKRGKWKTQVV"
     gene            complement(3817..3990)
                     /locus_tag="PLB46_04015"
     CDS             complement(3817..3990)
                     /locus_tag="PLB46_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HRF75714.1"
                     /translation="MLRYALIFFIVALVAAVFGFTGIAVGAASIARILFFIFIVLFLI
                     SLIGGYSFGKKKS"
     gene            complement(4172..4798)
                     /locus_tag="PLB46_04020"
     CDS             complement(4172..4798)
                     /locus_tag="PLB46_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRF75715.1"
                     /translation="MKKNYNCILILLLIISASQQLSGQYVTFGRAFSFGDAFITDKDV
                     SHGFLLSLTGGLSITYADNEHYGFAADVVYATEGSNSKTAGVAGDIQNKTKLGYIRIP
                     FKYIRFLGKYGDPIRPKIFVGPSFGYLVLAETNRDNSISDFNKFDMGLHIGVGANKII
                     CDRIWLNTDITYTQGILDVTKQTPLNDEINLNGSVRLNISLLLGTNRK"
     gene            5105..5455
                     /locus_tag="PLB46_04025"
     CDS             5105..5455
                     /locus_tag="PLB46_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75716.1"
                     /translation="MSNKTTVFAVLAGIGAGAALGYWFASEKNDKLRKKISKALNSVS
                     DDIKEKLINEFDELRMKAGEFKGKGVTLKDQILQSIVDMKEEGKQKVLDFIDKTHAET
                     NKIHDNGKQAVKQL"
     gene            5460..5843
                     /locus_tag="PLB46_04030"
     CDS             5460..5843
                     /locus_tag="PLB46_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75717.1"
                     /translation="MNTPDKNELRDFADDVKEYINVRLQIIGLNVTEKVAFALSNFIT
                     NGLSIILLLIMVVFLSLGGAFWLGTLVENTALGFVLMGAFYLFVFILIRYFLSKGIKA
                     IIMNILIRDLTNDNDDDDPDNKIEN"
     gene            5833..6261
                     /locus_tag="PLB46_04035"
     CDS             5833..6261
                     /locus_tag="PLB46_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75718.1"
                     /translation="MKIKNLNELRHERFLLRSRSEVLESEIKVQYREIADQVKPVLSV
                     IKRVTHLKDTISDKFNMDNEQPALKKGMQIAKQALPLVPIIAGNMIFNKGRKLIFQSI
                     AGYGLTQLTRYIFSKNGSEHAESIKGVFSFNKKDQEKGIF"
     gene            6351..7904
                     /locus_tag="PLB46_04040"
     CDS             6351..7904
                     /locus_tag="PLB46_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75719.1"
                     /translation="MAKTTFNTSLFILNFCFICTFPINSYSQAGELDLGFGIDGIVKT
                     DFGTTNDDKSYAVLVQPDGKIITAGYTFTTFSNDFAVARYNYDGSLDVDFGTDGLVTI
                     DFNGEKDFGYAAQLQPDGKILVAGTKCCFDDFGMIRLNTDGTLDNTFGGDGKVETDFF
                     GNLDNGLSIAIQTDGKIVMGGEAYGADRMFGLIRFNADGTIDDSFGVDGKVTTDVGIG
                     HDYLKSITLQADGKILAGGYYDTEGSDYHDYAIVRYNTDGTIDNSFGVDGFVKQDIYH
                     DQDFGFSIKTDADDNVLLAGYSDTVDGKYFTILRYTSAGLLDNTFGENGITTINFGDI
                     SYCKDIVLQPDGKIILAGFKVITDPDYEIALTRLNTDGKIDTSFGENGLVITDVGPEA
                     DYVYAVALQPDSKILAAGYIYNAGYDFLMLRYTGDKKVAIHNNLPNEQIIAVNPNPFN
                     DQFAITKYNSNSNKGELVMYNLSGQTLLKEIINNSETLIDAGKYPSGIYFLKYTEGNF
                     VQNIKIIKN"
     gene            complement(8075..8149)
                     /locus_tag="PLB46_04045"
     tRNA            complement(8075..8149)
                     /locus_tag="PLB46_04045"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8112..8114),aa:Pro,seq:tgg)
     gene            complement(8315..8404)
                     /locus_tag="PLB46_04050"
     tRNA            complement(8315..8404)
                     /locus_tag="PLB46_04050"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8368..8370),aa:Ser,seq:gct)
     gene            complement(8602..10038)
                     /gene="dnaA"
                     /locus_tag="PLB46_04055"
     CDS             complement(8602..10038)
                     /gene="dnaA"
                     /locus_tag="PLB46_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRF75720.1"
                     /translation="MSSKDFQTVWANCLKIIKDNVNLQSFKTWFEPIEPVKLEDEVLT
                     IQVPSQFFYEWLEEHYVTLLRKTIKRELGNNARLEYRIVVENSSGNNSPFTIDYPNYN
                     TGNNKNPEVAAPLVMGTSIKNPFVIPGLKKVNIESGLNANYNFDNFIEGDCNRLCRSA
                     GYAVAQKPGGTAFNPLVIYGATGLGKSHLAQSIGNEVKQNFPNKTVLYTNAERFTNQF
                     IESLKNNSVNDFVHFYQLIDVLIIDDIHFFANKAKTQDIFFHIFNHLHQESKQIILTS
                     DRPPRDLEGVEERLLSRFKWGLSADLQAPDFETRVAILEKKMYADGIELPKDVVEFVA
                     YNITNNVRELEGALVSLLAQASLNKKQIDLDLAKKIVKNFVKNMSREVSIDFIQKAVC
                     DYFEVPVDKLKEKTRKRQVVQARQLSMYLSKNFTKHSLKAIGKHFGGRDHSTVIHSCQ
                     AIQNLMDTDTKFKESVDDLQKKIQMSIS"
     gene            complement(10118..>10524)
                     /locus_tag="PLB46_04060"
     CDS             complement(10118..>10524)
                     /locus_tag="PLB46_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75721.1"
                     /translation="IELKGLVPEKLYDKIQYLDPVEKAKEMVAILRDKEKCDYIICLS
                     HLGYQYKDDKISDVKLAAQTEGIDLILGGHTHTFLDAPVIYKNLADKQVLINQVGWAG
                     VWLGRVDIFFEKKRTKKWHSGKPVKVSKKTRE"
BASE COUNT         3442 a         1992 c         1817 g         3273 t
ORIGIN      
        1 atacaagcct tttacatcac gtgcttcaca aacttctaaa ggcgcattta cataaacttc
       61 tacaaaatcg gtttccccaa taatgttttt ggccaaagtc ctgatttcat tggtcgggct
      121 aacaaaactg ttcagggtaa tgatgccgca gttcaaaaac aatttgctta cctcggcaat
      181 acggcgaatg ttttcaaccc tgtccggctc actaaaagtg aggttgttgt tgattcctgt
      241 gcgaatattg tcgccatcaa gcaacatggt caaataacca cgttcatgta aaattttctc
      301 caccgcttct gcaatagtgc tctttcctga accactcaaa cccgtaagcc atatgacttt
      361 cgagcgttgt ttcagcattg tctctttgtc gcctctttgt aaaagacggt caaaaatcgg
      421 atgtatattg tcctgaacag ggctcatatt gtgcaaatct gtgttaaaag cggtgcaaag
      481 ttaaggattg atgccgagga tttgtaggaa aacaggaaag aattggaaca aagtgaggct
      541 aatattctat taagaaagta gaatagtaat aaaaacgcta gaaaccacgg ctactaaacc
      601 gcaaaacacc aaagaatagg gaggttttct tttttggagc agggcaatct gaatggttat
      661 agcatgcaca acgggctttc gctatctagt gtgagcagag caagcattcc gcccatatca
      721 agaccaatta gctgtcaatt cgcacaagcc caaccgcatc aatcccggct aaccgtgtat
      781 tatctgccgg tcagcacatt tcagttccga cattatttat taaatccact atttcacctc
      841 ttcacttccc tgcaaaaagc gggttgaggg aattataaac caccaaggca tcatcttcga
      901 agacatttga aaaacattat tgattaaccc tggaattgca gttcttttcc ctttcatcat
      961 tgcagcatat ccttttttag caaccgggag cgcatcgggc accggcatga gtttcaataa
     1021 gcgggaacta tgcatatccg ctgcatcctg aaatcccgtt ttggttggtc ccgggtggaa
     1081 agaagtaaca cttacaccat aaggttttaa ttcattgtat aacgccgagg aaaaataata
     1141 aacgaaagct tttgttgcgt aataaattgc catggtagga ccgggctgaa aagaagccgt
     1201 gctggcaata ttcaaaattt ttccgctgcg agcaactacc atatctttta caaacaattt
     1261 agtgagatgc gccagcgcag tcatgtttaa ctgcatcatt tgatgttctt tttcccaatt
     1321 ggtattggca aacaacccaa aatccccaaa cccggcatta ttgattaaat aatcaacttt
     1381 taattgtttg tttttcacct catcataaac ttgctgagca gcatcaggaa tactcaaatc
     1441 ttttacaata atattaacct gaatgttatg tttagcagtt aattcctgct ttaattgttc
     1501 cagcttattt ccgcttcggg caactagaat taaatcgcca ccattagcag catgtatttt
     1561 agcaaattcg agacctaatc ctgaagaggc tccggtaatt aatgcagttg gcataattta
     1621 ttttttgtgg ttgaatttta aagtaattat tacagttttc gggcagggta tctatgttga
     1681 cgagttgctc aattttatat tgcaattgaa cacaaaattg cggtaaaagt ttagtttttc
     1741 tcggcatgca attttgacag atatcaaaaa cctgtcaggt ttaaacctcg gaggtttaaa
     1801 cctgacaggt tcaatttttt ttatccgcgc ttacacgttt aaatcaaatc cggctcaata
     1861 aaacaaatgt gccatgtgag taaaaatgtt taaatcgacc ctctgtaaat ttgtcaggta
     1921 ttggtttata aaaaaattta tttttttatt tataccacct gggttttcca tttgcctctt
     1981 ttaaaaataa ttacaaaagc aattgccata caagcctccg aaattgggac agccatatat
     2041 acgccggttg attttaaatc caacactgtc gacaaaaaat aagcaagcgg aatttgtaat
     2101 atccaaaacc cgaaaaaatt cacaatggta ggtgttttgg agtcgcctgc accatttatg
     2161 gcattggcca aaaccatttc gtacccataa gcaatatatc ctaaacataa aatccgcagg
     2221 cataaaacac cttcttttac aactgcttca tcgcgtgtaa taatatggat aagttgttca
     2281 ggaatggcaa aaaacaaaac cataacaaat cccataaata acatattaaa ataacccgtc
     2341 cgccagacag atttttcagc acgttcaggc aaattagctc cgagattttg acctaccaat
     2401 gttgctgctg cattggaaat tccccaagca ggtaaaatgg taaatactac aattcgtatc
     2461 gcagtggtgt atcctgccaa tacattttcg ccaaattcag acataatacg catcaaaaaa
     2521 atccagcttg cagaagcaat aataaattga cccgcagtgc ccgcagccaa tttcattaat
     2581 tgcgcaataa tatgtgtttt tatttgccaa atttttcctt tcaacacaat catcgacttg
     2641 ccgttaacga gataatacaa ttgatataat acgccaactg aacggccaat tgttgttgca
     2701 acagctgcac ctttaacacc caattccgga aaaatccaaa taccaaatat gaataacggg
     2761 tcgagaatga tattaattaa attcgccagc attaaactgc gcatggcaat tcctgcatca
     2821 cctgctccgc ggaaaattcc attaatcaga aacaataata aaatggcaaa attgccgcac
     2881 aacatccatt gaatataacc tttaccggtt tccaccattc catcagatgc gcccattaat
     2941 atcaatatgt ctcttgaata aaacataccg attaatgaaa taatcgttgc caatcctaac
     3001 cctaagtaaa ttgcctgcat acctgcttct gcagctccct ttgggtcttt ttcaccaacg
     3061 cgacgagcta ccaatgcagt agcacccata cttaatccaa atcctaatga ataaataatg
     3121 gtaagtgtac tttcagtgag tccaaccact gcaaccgctt caacactaat catgctgaca
     3181 aatatgatat caaccagagc aaaaatgctt tccatcaaca tttctgcaat cattggtact
     3241 gctaataaaa aaataacggt atcgatttta ccctgggtag catcgatatt ttcgcctttt
     3301 aaggcctttt tcaataatat aaaaaatgta cgtataccgg acataataat agtttaacaa
     3361 ataaaataat ttgagtaaac ctgtccgcat ttccaccaga tgggagacag ggtaagatac
     3421 cgggaaaaat taagctaacc gacttcggta aagtgaaagc ttatatttat aaaatcatat
     3481 gcctgaataa ttactgtggc aaatataaaa aaagattgca ataattgaaa actgaacaat
     3541 accaatggga actttcagga attgaatgac aaacggatgc catccgaaaa atggtataca
     3601 aaaagcgctt tgtagaatgt aagcgctttt tctcgaaata ctcaaatttt tcagtaaatc
     3661 agaagtatta aaacgatatt ttgagtgcta tttttccgaa caaaacatca cacaaccaca
     3721 ttataaaaca tggagccaaa attaaaaatc aaggcactca ctactcgtct aacaaatagt
     3781 aggttcgggt aaaaatgttt attgattgag ttgctatcag gattttttct tcccgaaaga
     3841 atatcctcca attaaactaa tgaggaacaa tacaataaaa ataaagaata aaattctggc
     3901 aattgaagct gcaccaacag ctatcccagt aaagccaaaa actgctgcaa ccaatgcaac
     3961 aataaagaaa ataagcgcat aacgtaacat aagcagtaat ttagaaagtt aaataaattg
     4021 ttaaaaatta tatcatacta taaacatgcc gtaaaaaact agtggctatt aacagttaaa
     4081 attgcattcg atttttatat atttcattat ttcgtgttta atccatctta aaatttgggg
     4141 atgtttttcc catattgcgg aaaacatcca cctattttct attagtccct aacaacaaac
     4201 taatgttgag acgaacactg ccatttaaat taatttcatc atttaaagga gtttgtttag
     4261 ttacatctaa aataccttgt gtgtaggtaa tatcagtgtt taaccaaatt cgatcgcata
     4321 taattttatt tgcaccaaca ccgatatgta aacccatatc aaatttatta aaatcgctta
     4381 tcgaattatc tctatttgtt tcagctaaaa caagatatcc gaacgatggc cctacaaata
     4441 tttttggtcg aattgggtcg ccatatttac ctaaaaaacg aatatattta aaaggtattc
     4501 tgatataacc cagtttagtt ttattctgta tatcaccggc tacaccggct gttttactat
     4561 tacttccttc tgtagcatac acaacatctg ctgcaaaacc ataatgttca ttgtcagcat
     4621 aagtaattga caatccaccg gttaaactta ataaaaagcc atgtgacaca tccttgtcgg
     4681 tgataaatgc atcgccaaaa ctaaatgcac gaccaaacgt tacgtactgc ccggaaagct
     4741 gttgacttgc actaattatt agtagcagaa ttaaaataca attgtaattt tttttcacaa
     4801 taaataaatt taaaaaatca gatacactaa tctttatagg tacaacatta caattaaaat
     4861 aattgatttt cgcaagaagt gataattctg taactatttg aattaattat gtaacaatgt
     4921 agcacctttc aatacctacc tttataattg taaaatatgg acatgtatta agctcaatta
     4981 gcatagcatg tttttatgaa caaactattg tatttaaaaa aacatggctt aatatttgaa
     5041 ttataaaaat caatagtgaa atcaaaaaac gtagcaattt atttaacaat ttaaatttag
     5101 aaatatgagt aacaaaacaa ctgtatttgc agtgttagcc ggcataggag cgggtgctgc
     5161 tttgggatac tggttcgcat cagaaaaaaa tgacaagttg cgaaaaaaaa ttagcaaagc
     5221 tttaaattca gttagcgatg atattaagga aaaactgatt aatgaatttg atgaattaag
     5281 aatgaaagcc ggtgaattta aaggaaaagg cgttacactt aaggaccaaa ttttacaatc
     5341 tatcgttgat atgaaagaag aaggcaaaca aaaagtactt gattttattg acaaaactca
     5401 tgctgagaca aataaaattc atgataatgg caaacaagcc gtaaaacaac tataacaaat
     5461 tgaatactcc ggataaaaat gaactacggg attttgcaga tgatgtaaaa gagtatatca
     5521 atgtgcgtct acagataata gggctgaatg taaccgaaaa ggttgcattc gccctatcta
     5581 attttatcac caatggttta tctatcattt tattattaat aatggtagtg tttttaagct
     5641 taggtggcgc attctggtta ggtacattag tggaaaatac agccttggga tttgtcctga
     5701 tgggcgcatt ttaccttttt gtatttatac ttattcgtta tttcttatca aaaggaatta
     5761 aagctattat aatgaatatt ttaattcgtg atttaaccaa cgacaatgat gatgatgacc
     5821 ccgataacaa aattgaaaat taaaaattta aatgaattac gtcatgaacg gttcttgtta
     5881 aggagccgct cggaggtttt agagtcggaa attaaggtcc aatatagaga aattgccgac
     5941 caggttaaac cggttttatc agttattaaa cgcgtaacgc atttaaagga caccataagt
     6001 gataagttca acatggacaa cgaacaacct gctctgaaaa aaggtatgca aatagcaaaa
     6061 caggctttac cattggtccc aatcattgcc ggaaacatga tttttaataa gggcagaaaa
     6121 ttaatctttc agtccattgc aggttatgga ttaacccagc ttacccgtta cattttttca
     6181 aaaaacggaa gcgaacacgc tgaatctatt aaaggtgtgt tttcattcaa taaaaaagat
     6241 caggagaaag ggattttttg atttacctgg ctctttatac gctcttccat gcataagtga
     6301 taaaatttct tttactaagg gaaaatattt tttatctttc ggtaaaaaaa atggcaaaaa
     6361 ctacatttaa cacttctctt tttattttaa atttttgttt catttgtact ttcccaataa
     6421 attcatactc ccaggcgggt gaacttgacc tcggttttgg tatagacggc attgtaaaaa
     6481 cagattttgg aacaacaaat gatgataaaa gttatgctgt tttagtgcaa cctgatggaa
     6541 aaattataac ggcaggatat acatttacta cattttcaaa tgattttgcg gtagcgcgat
     6601 acaactatga tggttcttta gatgtagatt ttggtaccga tggcctggta acaattgatt
     6661 ttaatggtga aaaagatttt ggatatgccg cacaacttca acctgatggt aaaattcttg
     6721 tagcaggaac aaaatgttgt tttgatgatt tcggcatgat tcgtttaaac actgatggaa
     6781 ctttagataa tactttcggc ggggatggga aagttgaaac tgattttttt ggaaatcttg
     6841 acaatggact ttctattgct attcaaacag atggtaaaat agttatgggt ggtgaagctt
     6901 atggcgcaga tcggatgttt gggcttatta ggtttaatgc tgatggtact attgacgata
     6961 gttttggtgt cgacggaaag gtaacaaccg atgtaggtat cgggcatgat tatttaaaat
     7021 ctattaccct tcaagccgat ggtaaaattt tagccggagg gtattacgat accgaagggt
     7081 ctgactatca cgattacgcc attgttcgtt ataatacaga tggcactatt gataatagtt
     7141 ttggcgttga tggttttgta aaacaggata tttatcatga tcaagatttt gggttttcga
     7201 taaaaaccga tgctgatgat aatgtattat tagcaggtta tagcgatacc gttgatggta
     7261 agtatttcac aattttgcgt tacacaagtg ctgggcttct cgataacact tttggagaaa
     7321 acggaattac aacaattaat tttggagata taagctattg caaagacatc gttttacaac
     7381 ctgacggaaa gataatacta gccggcttta aagtaattac cgatccggat tatgaaattg
     7441 ccctaacacg attaaatacc gatggaaaaa tcgatacttc ttttggagaa aatggattgg
     7501 ttataaccga tgttggtcct gaggctgatt atgtttatgc tgttgctctt cagcctgatt
     7561 ctaaaatttt agctgccggt tacatttata atgcagggta tgatttcctg atgttgcgat
     7621 ataccggcga taaaaaagtg gctatacata ataatttacc taatgaacaa atcattgctg
     7681 taaatccaaa tccgtttaat gaccaatttg caattacaaa atataacagc aattccaata
     7741 aaggcgaatt ggttatgtat aacttgtcag ggcaaacatt attgaaggaa ataatcaaca
     7801 attctgaaac attaattgat gccggtaaat acccaagtgg catctatttt ttgaaatata
     7861 cagaaggcaa ttttgttcag aatatcaaga taatcaaaaa ttagtccggc attattaaaa
     7921 ttaaccctca cttatttgag cttattaatt tggaccatgc tgccaactcc cttcatgcgc
     7981 gcacaattcg ggcttgtggg ttgatcatgc ttttctaaaa aataaggata aattggtaca
     8041 taaaaaaacc atcaactaag ttgatggttt tgtgtcgggg cggctggatt cgaaccagcg
     8101 gcctcctgct cccaaagcag gcattctaac cgggctgaac tacgccccga acaatatctt
     8161 ttttgacctt gcggccttcc cgcctaagcg ggacattcta accgggctga actacgcccc
     8221 gaacaatatc ttttttgacc ttacggcctt cccgcctgag cgggacattc taactgggct
     8281 gaactacgcc ccgaacggtt atttttcaat atgatggcgg tgagggagag attcgaactc
     8341 tcggtaccgt ttccagtacg gcagtttagc aaactgctgg tttcagccac tcacccacct
     8401 caccgggtaa ttagttaaaa tcaattcata tcttcaaaac ctaaagaaca actcaataaa
     8461 accctggctt ttaagcttgt tttctatcga aaggacggca aaggtaataa aacggttttt
     8521 tttatcctaa aaatttatgc attactgcaa aataaaatgc ctcccggttg aggaggcact
     8581 catttttatc gtttttgggc attatgaaat gctcatttga atcttctttt gtaagtcgtc
     8641 aacactttct ttgaacttgg tatcggtgtc catcaggttt tggatggcct gacagctatg
     8701 gataaccgta gagtggtcgc gaccgccgaa atgtttacca attgctttca gtgagtgttt
     8761 tgtaaagttt ttagacaaat acatactcaa ctgacgagcc tgaacaacct ggcgcttgcg
     8821 cgttttctct ttaagcttgt caaccggcac ttcaaaataa tcacaaactg ctttttggat
     8881 aaagtcgata gacacttctc gtgacatatt tttcacgaag ttcttcacaa tcttcttagc
     8941 taaatccagg tcaatttgtt ttttgttcaa tgaagcctga gcaagtaatg ataccagtgc
     9001 accttctaat tcacgaacgt tattggtaat attataggct acaaactcaa ccacatcttt
     9061 tggtaactcg ataccatcgg catacatttt cttttcgaga atggctacac gtgtttcgaa
     9121 atcaggtgcc tgtaaatcag cacttaatcc ccatttgaaa cggctcaaca aacgttcttc
     9181 aactccttcc aagtcgcgcg gagggcgatc gctggttaaa atgatttgtt tgctttcctg
     9241 atgtaaatgg ttgaagatat ggaagaaaat atcctgcgtt tttgctttat tggcaaaaaa
     9301 gtgaatatca tctataataa gtacatctat taattgataa aagtgtacaa aatcgttaac
     9361 tgaattattt tttaacgact cgataaactg atttgtaaaa cgttcagcat tggtatacaa
     9421 taccgttttg ttcgggaaat tttgtttaac ctcgttacca attgactgtg caagatggct
     9481 tttaccaagt cctgttgcac cgtaaattac taaaggatta aaagcagttc ctcccggctt
     9541 ttgcgctaca gcataaccag cacttctgca taaacgatta cagtcaccct ctataaaatt
     9601 atcgaagttg taattggcgt tcaaccctga ttcgatattt acttttttca aaccgggtat
     9661 tacaaacgga tttttgatgc ttgttcccat aactaatgga gccgctactt cagggttctt
     9721 gttgttaccg gtattgtaat tcggataatc aatagtaaaa ggggagttgt ttccagagct
     9781 attttctact actatcctgt actctaaccg ggcattgttt cctaattcac gtttaattgt
     9841 ttttcttaat aaagttacat aatgttcttc taaccattca taaaaaaatt gactgggtac
     9901 ctgaatcgtt aaaacttcat cctccaattt aaccggttca atcggctcaa accaggtttt
     9961 gaaactctga agattcacgt tgtcttttat tatcttaaga caattggccc acacagtttg
    10021 aaaatctttg ctactcatct cagaatttag aattttttaa ttatttattc acagcgggat
    10081 aacgaaggtg tgaaaaaaat atttctaaaa aaaattttta ttcccttgtt tttttcgaaa
    10141 ccttgacagg tttaccagaa tgccattttt ttgttctttt tttctcaaaa aaaatatcaa
    10201 cccttccaag ccacacccca gcccaaccta cctgattaat caacacttgt ttgtcggcaa
    10261 gatttttgta aattacaggg gcgtctaaaa aggtatgtgt atgcccccct aaaatcaaat
    10321 cgataccctc agtttgtgcc gccaacttaa catcggaaat tttatcatcc ttatattgat
    10381 accccaaatg cgacaaacat attatatagt cacatttttc tttatccctt aaaatggcaa
    10441 ccatctcttt tgccttttca acggggtcaa ggtattgtat tttatcatat agtttttcag
    10501 gcaccaatcc tttcaattcg atac
//
LOCUS       DAORKW010000059        10492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_128_length_10492_cov_4.334004, whole genome shotgun sequence.
ACCESSION   DAORKW010000059 DAORKW010000000
VERSION     DAORKW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128_length_10492_cov_4.334004"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PLB46_04065"
     CDS             complement(<1..767)
                     /locus_tag="PLB46_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HRF75722.1"
                     /translation="MALLYNRISHKELKQRMLESDEKRMTVSFYEYFPIEDPKLFRDT
                     LFADLSPLGVMGRIYVAHEGINAQMSLPVEQFDNFKKYINSVVHSTQFTDPVTGEKQL
                     RLNVAVDDDGKSFFVLKIKVREKIVADGINDPSFSMQNKGKYLTAEAYNQLSENPDTI
                     IVDMRNHYEYEVGHFKNAIEVPSDTFREQLPMAADMLKDKKDKPIIMYCTGGIRCEKA
                     SAYMLHNGFKNVYHIEGGIIHYANTIKEQHLDNKFIG"
     gene            944..2965
                     /locus_tag="PLB46_04070"
     CDS             944..2965
                     /locus_tag="PLB46_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75723.1"
                     /translation="MKKRILLPLLLISSSVAFSQTIIGNTSYDVQTNNAAKHRISVYD
                     DGSISAAWTGSTDYAVGTTNPDRGMFFNHYNGATWGAFPATRVETTKTGFGEVITVLD
                     HEVTLAHDGAALSIQLYANSSIGGTTWTELTGSDDVTGFWPAAVCPEGTNDIYMVNAN
                     ANPPTELRFSRSDDGGLTWSVLNSTVPFLTAAEGIPGLGVGLVAAAETYQIAVSGTNV
                     YILYGVPNSDLVLISSNANGAVGTWTSTVLHNFPIDNYNGLTQTDVDGDFVTDVIETT
                     DGNHNIIVDGDGNVHVYTGYVNIFNDGIGAFWSYDYSAPMGVWQWHTGMAEAELVDLT
                     LDWDNTDGLGDPDAGLGAFRSHYRYPGIVSTPGACIDPATGRIYLTYTMAIEYTDLFG
                     DPTNGSAQSFRDIFGVYSDDMGATWSTPVNLTNTAETGKENFYVSVYPEFKGGKVHMV
                     WQEDIYPGTTITDLDPIGVNNIMYNGFTAADFGDAPVVCNDITGPGGLYADGITSNSA
                     VMHWDAVAEADQYIVAFWNVADMANVGKRRPNTNMYAIPEGKLAPETTYGFRVKTVCY
                     DEGTISPYSDIAYFTTLPLREGETRESINVFPNPSNGVFTIELNGYENASVNTVIINA
                     VGQIVYENTSTPSAYNFTLPVNLNGVPTGIYYVTSTCNGKIVTNTIMID"
     gene            3069..5441
                     /locus_tag="PLB46_04075"
     CDS             3069..5441
                     /locus_tag="PLB46_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF75724.1"
                     /translation="MKKKLLLLVAVIYSFNLFAQTTVGTTTYDVQTNNSAKHRIIVYD
                     DGTVSTVWTGSTDLTGTFPDRGMFYNRYEGAWGAAPTLRAESVRTGFGEILNVEDHEL
                     IIAHDAGANKIQLFENDAIGSTTWTETAGSDDIRGIWPVAYSPASSNIVYVVCADTQI
                     ITSMIFSRSDDGGESWNILNYTLPFLTEADGVPIITNAAENYQVAAYENHVYVLFGMI
                     NSDLILLHSDDYGNDGTWEKTDIIDFPFDNYTGTVQTDIDGDLVTDTVNTTDGSHNMI
                     IDDDGIVHVFSPLYRIYSDAGAFFWTVNWNTMGMWHWSSGMPAAEIIDIELDWVNATC
                     DNNPYAGIGASTFNYRSAAPATNPAAAIDAASGVLYLLYAMKIEYTDEFDDPTNPSAQ
                     SFHDIFGMYSNDGGGSWTDPVNLTKNAEDGVENFYLNIYDRMVDGKIHAIWQQDNEPG
                     HFNEGDPITTNNMLYQSWDSESFTPTLPTANFTAAIDIEEVTFTNLSTDATGCYTWDF
                     GDGTFSNEINPVHTYLTADNYTVCLTAENPYGTDNYCTTINVILPPDAAFTYTGDPIV
                     SFTDLTLNDPTSWSWVFGDGGTSTLQNPTHTYLTEGTFTVCMTATNALGFEVYCLPVT
                     ITNTEALIPAADFDVSFVALTGVFTDMSTNSPNAWSWDFGDGGTSTEQNPSHTFAVSG
                     NYTICLTATNDFGSDDSCRQINTNAAFNFETVQLSLSPNPANDLVQIVMDNNTIYTVI
                     AYDITGRAIPLTYTQQSNIIVADVQSLPAGNYLLQIMDADKIGIGILQKQ"
     gene            5611..7218
                     /locus_tag="PLB46_04080"
     CDS             5611..7218
                     /locus_tag="PLB46_04080"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF75725.1"
                     /translation="MGSNIFKRTLLIIFIGLLHFQYAIGQDYIIRIMSANDKELALIE
                     CDNYYDYYNGFAAVKTDSCWTFFDEKGVAHFRNLNLISAENFADNGLAMVQTESGYGF
                     INKTGKFVVQPIYSSAYGYDFSNIALVENDNKWGVLDAQGKLIIPIEFDGIQSLGAAI
                     AVIKESSVAFYTNTGKPITGFDYNGYGSSNYDVVSVRSDAGWSFLDKYGNVSAPQYFD
                     EIGNYAEGRIGFIRYGKLGYVNSKGVEVIPAIYESGDEFSGGLAAVRKDGLWGYIDTA
                     GTVVIPFTYTAAFKFYDGFASVKNGEAWLSINKKNEIVPEPIATNTSPKSTYIKPVTA
                     NERITLTYQKYNYKVEKVWLNYDQTSNDGKIYHLTTGQLIGDNIVFKGQNIVSGKFYK
                     DNKSTQLKAEDGIPMGLLSAAITNQFAVDVAHEEAAKSKQVNTNNAQVDYVAMYTTLL
                     EKMKQEEEQFDKYLDLSTEFDLDAATEEERVANKQKKLQPYVDLINEIVQTIDYLIAN
                     DPNMHSDLEAELNYHRKDVLQDLKWIE"
     gene            7313..7828
                     /locus_tag="PLB46_04085"
     CDS             7313..7828
                     /locus_tag="PLB46_04085"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRF75726.1"
                     /translation="MEFTLRPWLESDLESLVQHANNFQVAKFMTDGFPHPYSYENGRA
                     FIAFASKDKPTHIFAIVVNGKAVGGIGIHPQTDIMQKNAELGYWLGEVYWRHGIISRA
                     IVLMLKFAFDTYDITRVYARPFGTNIGSQKVLEKLGFQLEARIKNNIFKNGQFDDELI
                     YGFRKEDLQKL"
     gene            complement(8270..10348)
                     /locus_tag="PLB46_04090"
     CDS             complement(8270..10348)
                     /locus_tag="PLB46_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF75727.1"
                     /translation="MNQKETLELIDKKILLEAYRKMFTSKVMADVFNENRQTCIFVHA
                     NSRGHEAIQLAMGMQLKKSDWASLYYRDDSILMGMGWDPSQIMLQLMAKRDDPFSGGR
                     NYYCHPSYKGSDYPQMIHQSSATGMQAIPTTGVAQGLQYLEQQGLLDTASQNPLVVCS
                     IGDSAMTEGEVAEALQFAVLKQLPIIYLVQDNEWGISVSKEEARTMDAYEYAGGFKGL
                     RRVRVDGSDFLESWQCVRDAIGYVRINRKPVLIHAKVPLLGHHTSGVRMEMYRTEEDL
                     KEHSSRDPLPKLRQVLRDAGISDSEVQALEMEADAKTRADFAQVVASPDPDPATVDLY
                     EFAPSPITEEQGNREPEGAEVVMMVDAALFAMDEILTKHPEALLYGQDVGRRLGGVFR
                     EAATLAAKHGDHRVFNTAIQEAYLVGSTVGMSATGTKPIVEIQFADYIWTGVNQLVSE
                     LSKSYYLTNGKWNVQTVIRIPIGAYGSGGPYHSGSIESSITAIKGIKVVYPSNAADMK
                     GLLKGAFYDPNPVVMFEHKGLYWSKVPGTAAAKNIQPADDYIIPLGKARIFQHADAAK
                     IDNGESMLVVTYGMGVHWALNASKSFPGQIEILDLRTLYPYDWEAIETSVKKHNKVFV
                     LTEEPIMNSYAQAMAGRIGSELFKYLDAPVQMLGAKNLPAVPLNSGLEKAMLPNVDKV
                     KVVMEELLGW"
BASE COUNT         3160 a         2004 c         2035 g         3293 t
ORIGIN      
        1 ttaccgataa atttattgtc gagatgttgt tctttaatag tattggcata atgaataata
       61 ccaccttcaa tatgatacac atttttaaat ccgttgtgta acatataggc acttgctttt
      121 tcgcaacgaa tgccaccggt gcaatacatg ataatcggtt tatctttttt atcctttaac
      181 atgtctgcag ccattggtaa ttgttcgcgg aatgtatcac tcggaacttc gatggcattt
      241 ttaaaatgac ctacttcgta ttcatagtga ttacgcatgt caacaataat cgtatcgggg
      301 ttttccgaca attgattata ggcttctgct gtcaggtatt tccctttatt ttgcatgctg
      361 aatgatgggt cgttaatacc atcagcaaca attttttcac gcacttttat ttttaaaaca
      421 aaaaaagatt ttccgtcgtc atcaactgca acatttaatc gcaattgttt ttcaccggtt
      481 acagggtcgg taaattgtgt tgaatgcaca acactgttta tatatttttt gaaattgtca
      541 aattgttcaa ccggcaaact catttgcgca ttaattcctt catgtgcaac ataaattctg
      601 cccataactc ctaatggtga taaatcggca aataatgtat cgcgaaataa ttttggatct
      661 tcaatcggaa aatattcata aaaagaaaca gtcattcttt tttcatccga ttccagcata
      721 cgctgtttga gttccttgtg ggaaattctg ttgtataata aagccataat agaaattttg
      781 tatcccgtta caagcggggt gaaatctcaa taaacggtgc aaaggtacca ccaatctttg
      841 gcctttaaaa attttaacga ttgattaata ttcttggaaa tttcctttgt ttttaccaat
      901 tggattaagt aatattaact tttattaacc aaaagcactt ataatgaaaa aacgaatttt
      961 actcccattg ctgctgattt cctcatcagt agccttctcg caaaccatta ttggcaatac
     1021 atcatacgat gtgcaaacca ataatgccgc caaacatcgt atttcagtat atgacgacgg
     1081 cagtatttca gcagcctgga caggctcaac agattacgct gtcggcacta ccaaccccga
     1141 tcgcggtatg ttttttaatc attacaacgg tgctacctgg ggtgcctttc ctgcaacccg
     1201 tgtagaaaca actaaaacag gttttggcga agtcatcaca gtgctcgacc atgaagtaac
     1261 cctggctcac gacggtgcag cactttcgat acaattgtat gctaactcca gcattggtgg
     1321 cacaacgtgg actgaattaa ctggttctga tgatgttacc ggtttttggc ctgcagctgt
     1381 ttgtcctgaa ggaactaatg atatttatat ggtaaatgct aacgctaacc ctccaactga
     1441 attgcgtttt tcgcgttctg atgatggtgg tttaacctgg tcggtattaa attccaccgt
     1501 tccgttttta actgctgctg aaggtattcc tggtttgggt gttggtttag ttgcggctgc
     1561 cgaaacatat caaattgctg tttcaggtac caacgtatat atcttatacg gagtgccaaa
     1621 ctcagatttg gtattaattt cttcgaatgc aaacggtgct gttggaacct ggaccagcac
     1681 tgtattacat aatttcccaa ttgataatta caatggcctt acacaaacag atgtagatgg
     1741 cgattttgtt acagatgtaa ttgaaactac tgacggtaat cacaatatta ttgttgatgg
     1801 cgatggtaat gtgcatgtat ataccggtta tgtaaatatt tttaacgatg gtataggtgc
     1861 attttggtca tatgattaca gcgcacctat gggtgtttgg caatggcata caggtatggc
     1921 agaagctgaa ttagtggact taacattaga ttgggataat actgatggtc ttggtgatcc
     1981 tgatgcaggt ttaggtgctt tccgttcaca ttatcgttac cctggtattg tttctacacc
     2041 tggtgcttgt attgaccctg caacaggtag aatttattta acatatacga tggcaattga
     2101 atacaccgat ttatttggtg acccaacaaa cggttctgca caatcattcc gcgatatttt
     2161 tggtgtatat agcgatgata tgggcgcaac ctggtcaaca cctgttaacc tgactaatac
     2221 tgccgaaact ggaaaagaaa atttctatgt ttctgtttat cctgaattta aaggaggtaa
     2281 agtgcacatg gtttggcagg aagatattta cccgggaaca actattactg acctcgaccc
     2341 gattggtgta aataatatca tgtataatgg ctttactgct gccgatttcg gtgatgctcc
     2401 cgttgtttgt aatgatatta ccggcccagg tggattatat gctgatggta taacatcaaa
     2461 ctctgcagtt atgcattggg atgctgttgc agaagccgat caatatattg tggcattctg
     2521 gaatgttgcc gatatggcaa atgttggtaa acgccgtcca aataccaata tgtatgctat
     2581 tcctgaaggc aaattagcac ctgaaactac ttatggtttc cgtgttaaaa cagtatgtta
     2641 cgatgaagga acaatttctc cttattctga cattgcttat tttacaactt taccattaag
     2701 agaaggtgaa actcgtgaat caataaatgt tttccctaat ccatcaaatg gtgtatttac
     2761 catcgaatta aatggttatg aaaatgcaag tgtaaatact gttattatca atgctgttgg
     2821 tcagattgtg tatgaaaata ccagcacacc ttccgcttat aactttactt taccggttaa
     2881 tttaaatggt gttcctaccg gaatttatta tgttacttct acttgtaatg gtaaaattgt
     2941 taccaataca ataatgatag attaattacc aaaaaaaaca acacctctaa ataaattagg
     3001 ggtgttgttt tttaactact tcattgttgt ttttcccttc gtgtttttta acagctaaaa
     3061 aaataagcat gaaaaaaaaa ttactccttt tagttgccgt aatttattcc ttcaatttat
     3121 ttgctcaaac aaccgtagga acaaccactt acgatgtgca aacaaataat agtgcaaaac
     3181 accgcattat tgtttatgac gacggcaccg tttccacagt ttggactgga tctacagatt
     3241 taaccggcac atttcctgat cgagggatgt tctacaatcg gtatgaaggc gcctggggtg
     3301 ctgctccaac gcttagagca gaatcagtac gcaccggttt tggcgaaata cttaatgtgg
     3361 aagatcatga attaattatt gcccatgatg caggcgcaaa taaaattcag ctatttgaaa
     3421 atgatgccat tggttcaaca acatggactg aaacagcagg ctctgacgat atacgtggta
     3481 tttggcccgt tgcctattct cccgcatcgt ccaatatcgt gtatgtagtt tgtgcagata
     3541 cacaaattat tacttcaatg attttttcgc gctccgacga tggtggtgaa tcgtggaaca
     3601 tattaaatta tacgctgccg tttttaacag aagcagatgg tgttcctatt attaccaatg
     3661 ctgctgaaaa ttatcaggta gctgcttatg aaaatcatgt ttacgtttta tttggcatga
     3721 ttaattccga tttaatctta ttacattccg acgattatgg aaatgatggc acttgggaaa
     3781 aaacggatat cattgatttc ccttttgata attatacagg aacagtacaa acagatatcg
     3841 atggcgattt agtgactgat actgtgaata ccactgatgg ttctcataac atgataattg
     3901 atgatgatgg tattgtgcat gtattttctc cgttatatcg catttatagt gatgcaggtg
     3961 catttttctg gaccgttaac tggaatacca tgggtatgtg gcattggtcc tctggcatgc
     4021 ctgcagcaga aataattgat attgaattgg actgggttaa tgcaacctgt gataataatc
     4081 catatgcagg aattggagcc agcacattta attatcgaag cgcggcacca gctaccaatc
     4141 ctgctgctgc aatagatgct gctagtggtg ttttatattt attatacgca atgaaaattg
     4201 aatataccga tgagtttgat gacccaacta atccgtcagc tcaatcgttt cacgatattt
     4261 ttggcatgta ttcaaacgat ggtggtggtt cgtggacaga cccggttaat cttacaaaaa
     4321 atgcggagga tggcgttgaa aatttttatt tgaatattta cgacagaatg gtagatggaa
     4381 aaattcatgc tatctggcaa caggataatg agccaggaca ttttaatgaa ggtgatccaa
     4441 taacaacaaa taatatgttg taccaatctt gggatagcga aagctttaca ccaactttac
     4501 caactgccaa ttttactgca gcaattgata ttgaggaagt aacttttact aatttatcaa
     4561 cagatgcaac cggttgttat acatgggatt ttggtgatgg tacattcagc aatgaaatta
     4621 atccggtgca tacttatctc accgcggata attacacggt ttgtttaacc gcagaaaatc
     4681 catatggaac tgataattat tgtacaacaa taaatgtcat tttaccaccg gatgctgcat
     4741 ttacttatac aggtgaccca atagtatcat tcaccgacct cacgttaaat gatcctactt
     4801 cttggagctg ggtttttggt gatggcggaa caagtacctt acaaaatccg actcatactt
     4861 atttaactga aggtacattt actgtttgta tgactgcaac aaatgcatta gggtttgaag
     4921 tatattgttt acctgttact attaccaata cagaagcact tattcctgct gctgattttg
     4981 atgtgagttt tgttgcattg accggtgtat ttacggatat gtcgacaaat tcaccaaatg
     5041 catggagctg ggattttgga gatggtggaa cttctacgga acaaaatccg tctcatactt
     5101 ttgcagttag tggtaattat accatttgtt taactgccac aaacgatttt ggaagtgatg
     5161 attcatgtcg ccagataaat acgaatgcgg catttaattt cgagactgtt caattgagtt
     5221 tgtcgccaaa tccggctaac gatttggtac aaattgttat ggataataat accatttata
     5281 cggtaattgc ttatgatatt acaggaagag caattccatt aacatatacg caacaatcca
     5341 atattattgt cgcggatgtg caatcattgc ctgcaggaaa ttatctgttg cagataatgg
     5401 atgccgataa aataggtatc ggaattttac agaagcaata aatgattaat tttatttaaa
     5461 ctctcggcat tcgccgggag ttttttattt tatgagcatt ccgcttgcga tacttgacta
     5521 ccaattaaac cttataaata ggtagggttt aagcggcaat ctaaatatgt tttttaatta
     5581 tacctttgtt tgctcaaaca aaatgaacat atgggatcca acattttcaa aaggacgctg
     5641 cttataattt ttataggtct attacatttt caatatgcca ttggtcagga ttatatcatt
     5701 cgaatcatgt ccgcaaacga taaggagctt gctttaattg aatgtgataa ttattatgac
     5761 tattacaatg gatttgctgc cgtgaaaaca gattcctgct ggacattttt tgatgagaaa
     5821 ggtgtggcac actttcgtaa tctgaattta atttcagctg aaaattttgc tgataatggt
     5881 ttggccatgg tgcaaactga atcgggttac ggatttatta ataaaacagg aaaatttgtt
     5941 gttcaaccaa tttatagcag cgcttatggt tacgacttta gtaatatcgc tctcgtggaa
     6001 aatgataata aatggggtgt gttggatgcg cagggaaaat taattattcc aatcgaattt
     6061 gatggtattc aatccctggg tgctgcgatt gcagttatta aagaaagtag tgttgcattt
     6121 tataccaata ccggcaaacc tattacagga tttgattata atggttatgg gtcttcaaat
     6181 tatgatgtgg tgagtgtaag aagtgatgcc ggatggagtt ttttagataa atatggaaat
     6241 gtttcggcac ctcaatattt tgatgaaatt gggaattatg ctgaaggaag aattggattt
     6301 attcgttatg gtaaattggg ttatgtaaat agtaaaggtg tggaagttat tccggctatt
     6361 tatgaatccg gggatgaatt ttcaggagga cttgctgcag tcaggaaaga tggtttatgg
     6421 ggatatattg atactgccgg tactgttgtt attccgttta cgtatacggc tgcttttaaa
     6481 ttttatgatg gttttgcatc tgtaaaaaat ggagaggcat ggctgagtat taataaaaaa
     6541 aatgaaattg ttcctgagcc gatagctacc aatactagtc ctaaaagcac ttatattaaa
     6601 ccggtaactg ctaacgaacg aattacatta acttatcaaa aatataatta taaagttgaa
     6661 aaggtatggc ttaattacga ccagacatca aatgatggaa aaatatatca tttaacaacc
     6721 ggtcagttaa ttggtgataa tattgttttt aaagggcaaa acattgtgtc aggtaaattt
     6781 tataaggata ataaatcaac gcaattaaaa gcagaggatg gtatcccaat gggtttactt
     6841 tcagcggcta taactaatca gtttgcagtg gatgtggcac atgaggaagc agctaaatct
     6901 aaacaggtta atacgaacaa tgctcaagtt gattacgtag cgatgtatac tacacttttg
     6961 gaaaaaatga agcaggaaga ggaacaattt gacaagtatc ttgacctcag tactgaattt
     7021 gatttagatg ctgctacaga agaagaacgt gttgccaata aacaaaaaaa gttacaaccc
     7081 tatgttgatt tgataaatga aattgttcaa acaatagact acttaattgc aaacgaccca
     7141 aatatgcaca gtgacctgga agctgagtta aactatcacc gaaaagatgt attgcaggat
     7201 ttgaaatgga tagagtaagt agatagcatt tatatcatgt agagggggga atttaatgat
     7261 gattattccc aaccggtatt tgccggataa atttatttaa taatttagcg gtatggaatt
     7321 cacacttcgc ccatggttgg aaagcgatct ggaaagttta gtgcaacatg ccaataattt
     7381 tcaggttgct aaatttatga ccgacggttt cccgcatcct tattcgtatg aaaatggcag
     7441 agcatttatt gcatttgcat caaaagataa acccacacat atttttgcaa ttgttgttaa
     7501 tggtaaagca gtaggtggaa ttggtattca tccgcaaacg gatattatgc aaaaaaatgc
     7561 ggagctggga tattggctgg gagaggtgta ttggcgacat gggattattt ccagagcaat
     7621 tgtattaatg ttgaaatttg cgtttgatac gtatgatata acccgtgtgt atgcccgccc
     7681 attcgggact aatattggtt cccaaaaagt gttggagaaa ttaggttttc aattagaagc
     7741 acgaattaaa aataatattt tcaaaaacgg ccagtttgat gacgaactaa tttatggttt
     7801 ccgaaaagag gatttacaaa aattataaat aattaaaaag ctcccagctc tgcaaaatct
     7861 ctgattctgc agcatcgtgc caggactccg tccgatgcgc tctatagata aaatttaatt
     7921 ttatggtatt aattagcaat taaatttccg aactaaacag ccgaaaccac aatcccgata
     7981 gctatcggga cacagagaga acccacacag agcccacgga gaaacccaca cagtgaacac
     8041 aaaaaaaact gctttgcaaa atctctgatt ctgcagcatc gtgccaggac tccgtccgac
     8101 gcactccaca aataaaataa tatcgttttt gtgtttatca aataaatttt gaaccagctc
     8161 tgcaaaatct ctgattctgc agcatcgttc caggactccg tccgacgcac tctataaata
     8221 aaattaaatt gaaaagtata aaccagcact ttaattgcgc actaaaaaat caccaaccca
     8281 acaactcttc catcaccact ttcaccttat ctacatttgg tagcatcgct ttttctaatc
     8341 ccgaattaag tggcactgca ggtaaatttt ttgcacccaa catttgtaca ggagcatcga
     8401 gatatttaaa taattcggaa ccaattcttc ctgccatagc ttgcgcatac gaattcatta
     8461 ttggttcttc agtgagtaca aatactttat tatgtttttt tacagatgtt tcaatagctt
     8521 cccaatcgta aggatataat gtacgtaaat cgagaatttc aatctgcccc ggaaaacttt
     8581 tcgaagcatt taatgcccaa tgcacaccca taccatatgt caccaccaac atactttctc
     8641 cgttatcaat tttcgctgca tcagcatgct gaaaaatacg tgcttttccc aaaggaataa
     8701 tataatcatc agcaggctga atattttttg ccgcagcagt ccccggcact ttactccaat
     8761 acaatccctt atgttcaaac atcaccacag gattcgggtc gtaaaatgca cccttcaata
     8821 atcccttcat atccgcagca ttacttggat acaccacctt aattcctttt atcgcagtaa
     8881 ttgacgattc aattgaccct gaatgatagg gtccgccact gccataagca ccaatcggaa
     8941 tacgaatcac cgtctgcaca ttccatttcc cattcgttaa ataataggat ttagataatt
     9001 cagaaactaa ttgattaacg cctgtccata tataatctgc aaattgaatt tctacaattg
     9061 gtttagtacc cgtagcactc attccaacgg tagatccaac caaatacgcc tcttgaatag
     9121 cagtattaaa tacacgatgg tcgccgtgtt ttgccgccaa agttgcagcc tcacgaaata
     9181 caccacctaa acgtctgcca acgtcctgac catacaacaa tgcctccgga tgtttagtca
     9241 aaatttcatc catggcaaac aaggctgcat ccaccatcat taccacttca gcaccttccg
     9301 gttcgcggtt gccttgctct tcggtaattg gcgatggtgc aaattcatat aaatcaacag
     9361 tggcaggatc cgggtcagga gaagccacca cctgagcaaa atcggctctg gttttcgcat
     9421 ctgcttccat ttccaatgcc tgcacctcac tatcggatat acctgcgtca cgtaatacct
     9481 gacgcagttt tggcagtggg tcgcgactgc tatgttcttt caaatcttct tcggtgcgat
     9541 acatttccat gcgcacaccg ctggtatgat ggcctaataa tggcacttta gcatgaataa
     9601 gcaccggttt tcggttgatg cgtacataac caatcgcatc acggacacat tgccagcttt
     9661 ccagaaaatc gctgccatct acacgaacgc ggcgcaagcc tttgaaacca ccagcgtatt
     9721 cgtatgcatc catagttcgg gcttcttctt tggaaacaga aatgccccat tcattatcct
     9781 gcactaaata aataatcggt aattgtttta atacggcaaa ttgtagtgcc tctgccacct
     9841 caccttcagt catggcacta tcgccgatag aacaaaccac cagcgggttt tgagatgccg
     9901 tatctaacaa tccctgctgc tctaaatatt gtaaaccctg tgcaacaccg gttgtaggaa
     9961 ttgcctgcat gccagttgcc gaggattggt gaatcatttg agggtaatcg ctgcctttgt
    10021 agctggggtg acaataataa ttccggcctc cgctaaatgg gtcgtcgcgt ttggccatta
    10081 attgtaacat tatttggctt gggtcccaac ccattcccat caaaattgaa tcatcacggt
    10141 aatataaact ggcccaatcg ctttttttca actgcatacc catagccaat tgaatagctt
    10201 catgcccgcg ggaattggca tgcacgaaaa tacaggtctg gcgattttcg ttaaaaacat
    10261 cggccattac cttgcttgta aacatctttc gatacgcttc aagcaatatc tttttgtcga
    10321 ttagttccag ggtttccttt tgattcatct tgcgggtgca aagctataaa ctaatagtgt
    10381 gggtagaaag ccgtttggtt gcttatttaa tgtttatttc aaatctttta gtcccttcca
    10441 aaaatccggt cagcacatca ccaatggcca caggacctac accggccgga gt
//
LOCUS       DAORKW010000060        10457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_131_length_10457_cov_4.919054, whole genome shotgun sequence.
ACCESSION   DAORKW010000060 DAORKW010000000
VERSION     DAORKW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10457
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131_length_10457_cov_4.919054"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PLB46_04095"
     CDS             complement(<1..470)
                     /locus_tag="PLB46_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF75728.1"
                     /translation="MSKKYLLLILTLVGLKMQAQLVINEYSASNWKQFIDNHNDYEDW
                     FEIYNGYPDDVDLVGYYLSDEDDNLTKWQFPAGSVIPANGFLNIWCSGRNTVDGEYYH
                     TNFRLTQTKNNPDQIIISNPGAVIVDSLTMDFTLTHHSRARALDGSPTWQLCTSP"
     gene            complement(806..1372)
                     /locus_tag="PLB46_04100"
     CDS             complement(806..1372)
                     /locus_tag="PLB46_04100"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF75729.1"
                     /translation="MSKISAPARRALEQEGISNLKQLSKYTEAHLLELHGIGKSAVKI
                     LLQELTANNLTFKIQTTKATSVKDKTKPESVNAYINSFPKDAQKYLQKIRTLIKKIIP
                     DVEEKISYGMPSYHYQGKYIIYFAGYKNHIGIYPIPTGNKELDGLYKNYKITGKATLQ
                     LPLGKPLPLDLIEKMVKHKLATLTNKLK"
     gene            complement(1539..3344)
                     /locus_tag="PLB46_04105"
     CDS             complement(1539..3344)
                     /locus_tag="PLB46_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRF75730.1"
                     /translation="METFNKKNGVSVRAYQGDAMTLLAFDLQYSLRKNLAGFTIRYSF
                     EVNGNLQHAFLYNRMIFPDSFYQINQVPFTEKNSTLYAPIQKMNWVHVPNTNINTSLP
                     IFGNYTYEITPRYIKNGVLLPLNTELTVAVTIYVGPFKVKNTELGFTRGFVSSVAYAK
                     RFNVKNNRVRPPKTKDELLFDVKQVADTAKRWNDTKRQYETVPYTFEEQHKWLGWQAR
                     ERILTFLDEAVADTTILVKAFVYDLNEPEICKRLLELGKQGRLQIILDNAGAHGDSQS
                     METQFERKFKYVAPDNNAIYRGKFKALAHSKVFIQIKNDKAIKVLTGSTNFSTNGLYI
                     NSNHVLIFSDNTIAKLYQDVFDASFGETNMKSFYGSSWSTGEFSFSGNSTPKMTITFA
                     PHTKDAAQTIFDRIRKRISVASNTDVLFAIMKDTNASAILDAVREQVRNEKIFTYGIT
                     DSIGDNAEYSVFLYKPNALRGVRVAAKGISNTLPPPFGTVAGIDGYAIHHKFIVVNFK
                     GKDPTVYCGSSNLSFTPEQKNGDNLLEIHDDDIVTAFAIEALRLVDHFHWRNNELSDK
                     PMFLDDLSDNSEMWYEAWFNRKDLKNRQRKLYIKA"
     gene            complement(3538..4044)
                     /locus_tag="PLB46_04110"
     CDS             complement(3538..4044)
                     /locus_tag="PLB46_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF75731.1"
                     /translation="MINIATPVTTSRMVLQPIAVDDHEFVLQLVNSEGWIQFIGDRNV
                     HSEEDAKAYIHKIQQTPNLVYWVARLNDNQLPVGIISYMKRAYLDNYDLGFAFLPEYQ
                     QQGLAFEAASGVLDFVINKIHEKTILATLLPENVKSIRLLLKLGFQFSATVDVENERL
                     HIYQLNIT"
     gene            complement(4126..5307)
                     /locus_tag="PLB46_04115"
     CDS             complement(4126..5307)
                     /locus_tag="PLB46_04115"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRF75732.1"
                     /translation="MKNILLILVLGLLFAACRKGEVIEPVFYTCGFGYIDSSDNNIND
                     GPYQNLLDNITANGVVGIEMAVYNNTDGMWVGASGKADLFNDVDMQPCNITRVGSTVK
                     MFTATTVLLLQEEGKLNINDKISNYLDGIYMDKIENASTATIEQLMHHSSGIFNYIHN
                     LQFQTASLNDLLKPWEAEELLQYAYNKPAYFSPGDDVTYSNTNYILLGLLIEQIEGKP
                     LYQVFDEKIFQPLGLSSTQFAGKNPVPDGIVRGYIDIYSNLQVIESTYYSGWDYYTAD
                     GGLISTAYDLNVFLRALMQGQIINSTSLDAMFSWQTPKNLDPEFYDIAYGLGIFKMVT
                     PMGECYFHSGDAIGYYANMMYFPEDSTTVVYAVNSNYGKIDEFVSTKDAITNILNVTK
                     H"
     gene            complement(5304..5903)
                     /locus_tag="PLB46_04120"
     CDS             complement(5304..5903)
                     /locus_tag="PLB46_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75733.1"
                     /translation="MKLLLHPMFFFVAVSLHAQQKNIPIQISLYNEATAIPFTKLVTT
                     PIHPGIQIGTEFTYKESEHFRLFQTVNLSYYFHAQLNHGISVNTELGGEYLTNSRFSF
                     GALIGVGYLHTLATTKEYVFEDGHYTIATDKGNARIAPSLSFDVNYQLNKIEPTSPKI
                     FVRYQSWIEYPYSPGFIPLMSHINLHIGAKFNLPSNTGK"
     gene            complement(5993..6697)
                     /locus_tag="PLB46_04125"
     CDS             complement(5993..6697)
                     /locus_tag="PLB46_04125"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HRF75734.1"
                     /translation="MNTNKSIARKAGLIYLIVVISGIFSLMYVPNQLIDLNNAAVTYQ
                     NIQSHELLFRLGILGNVICYLAFMILPFALYKLLKNIHPNMAKSMVILVLIGIPIAFV
                     NLQYKFAVLSMLNNEHLFAGIPINQLQQQLLISLQNFNNGINLLSVFWGLWLFPFGWL
                     VYKSGFLPKLLGILLMVGCMCYLIDFTASLLAPKYNSSGISTFVMLPASIGEIGICLW
                     LLIVGVSKSKGPDQHV"
     gene            complement(6703..7137)
                     /locus_tag="PLB46_04130"
     CDS             complement(6703..7137)
                     /locus_tag="PLB46_04130"
                     /inference="COORDINATES: protein motif:HMM:NF039408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HRF75735.1"
                     /translation="MPENQPIRSKFKDYEVNTKIKLAFLWTAVTFCYLYGDYFELYTP
                     GKVEGLISGNNLLNTPLNLLLATVMLAIPALMVIVSVFLRPAINRILNMIFGLIFTLI
                     MFSIVIGATSEWYRFYFLLGIIEMVITLLIIRVAFVWPKNQS"
     gene            complement(7225..8052)
                     /locus_tag="PLB46_04135"
     CDS             complement(7225..8052)
                     /locus_tag="PLB46_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75736.1"
                     /translation="MNHQKNLQGLICLFVIVLSSCKEDFPVNANKTNFKIPVVIQKKT
                     DDTIGYFKAISISDAHNRYEGKRKFCDTLDINISRHKDSLEKSDYVSGRDVWRADTFG
                     YDGLELFPDYSSTIIRDDLLHEQLKNYYPVYIVNQTPNVKFIIGKDSHVFAIQEAVDT
                     AGHWRPIEGRGFDFCGFGYWGVKIHPQEFVTILMPRYEGEFTTKLRVRVKIGNMIYIS
                     KSFTGKIDERQFYIDEEDYFFSEFKRDNVNTIQHIFYGAIPAQMDNADSELHALITQ"
     gene            complement(8218..9099)
                     /locus_tag="PLB46_04140"
     CDS             complement(8218..9099)
                     /locus_tag="PLB46_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRF75737.1"
                     /translation="MVTQDTAKKQAIRLVECPRDAMQGLHDFIPTDKKVAYINQLLKV
                     GFDTIDFGSFVSPKAIPQMADTADVLAQLNLSETNSKLLAIIGNVRGAEDAVKHKQIT
                     YLGFPFSISETFQQRNINSSITESVERVKEIKQLCDDNGKKLMIYISMGFGNPYGDPW
                     SAEVAAEWVEKLVTMDIKYVSLADTIGVANPESITYLFSHLIPKFPGVDFGAHLHTTP
                     DAWLEKIDAAYKSGCTRFDGAIKGFGGCPMAADDLTGNMPTENIIQYFDHQKVSMGIN
                     KYEFRESVRKALVTFPV"
     gene            complement(9121..9648)
                     /locus_tag="PLB46_04145"
     CDS             complement(9121..9648)
                     /locus_tag="PLB46_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75738.1"
                     /translation="MRIIFSCYLVSISILSFGQSQVVMQQTIKNQATTMLDAFEDHNY
                     EKLLDFTYPKIFELSGGKATMLEIIQQSMQELELAGYIVDSANIGEPGPIYNAGTELH
                     AIISQFVYMTIPGGSMMNESPLLAVSDNNGVSWYFLDTKQLDGNLKAYFFPNFNEDLK
                     IPEPKPVIYDYEKKK"
     gene            complement(9690..>10457)
                     /locus_tag="PLB46_04150"
     CDS             complement(9690..>10457)
                     /locus_tag="PLB46_04150"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRF75739.1"
                     /translation="DFTDYQGSFGILEEMQTINFIASSSITEKMSKSKYNVVNPDDVI
                     NEYGADCFRMYEMFLGPIDAGKPWDTKGITGVQGFLRKLWRLFIDEQGQLRITNEAPT
                     TAELKLLHKTIKKAGEDIEKLSFNTAVSAFMICVNELQSAKCYKREILEQLLILVAPF
                     APFITEELWEKCGHTDAVHIQSWPKYDATYLTESNFNYPVSINGKVRANIELPLDMEE
                     NEIRETVLGLESVVKWTEGNTPKKVIIVKGRIVNVVL"
BASE COUNT         3383 a         1949 c         1649 g         3476 t
ORIGIN      
        1 ggtgatgtac aaagttgcca tgttggactt ccatccaaag ctcttgcacg ggaatgatgt
       61 gtaagggtaa aatccatagt taatgaatca acaattaccg ctcccggatt ggaaataata
      121 atttggtcgg ggttattttt agtttgtgtt aagcgaaaat tcgtatgata atattcacca
      181 tccactgtat ttcttcctga acaccatatg tttaaaaaac catttgccgg aattaccgac
      241 ccggcaggaa attgccattt ggtgagatta tcatcctcat cacttaaata atagcccact
      301 aaatcaacat catccgggta tccattataa atttcaaacc aatcctcgta atcgttatgg
      361 ttgtcgataa attgtttcca gtttgaagct gaatactcat taataaccaa ctgtgcttgc
      421 attttcagac caaccagggt caggataagg aggaggtact ttttcgacat gaaattaatt
      481 ttttgtaaat atatagttaa gttcacgtaa attatagttg catatagcag taaatatgaa
      541 tttaggatga caaactataa agggatactc tttttaagtc aatttaacat ttttgcgatg
      601 tttaaagtag gtgaaatagg tatcaagtaa taacaattga aaatctgcct caaccggata
      661 acaagggtaa aaggcatatc agttaccatc ttagtcaaat gcagaaatta tgaattaaac
      721 ctgcctacta tggaaagaac aatttcagct taatgcataa ttaccctttc aattttgttt
      781 acaaattaat taaacaatcg ctgctttact ttaatttgtt tgttaaagta gccagtttgt
      841 gttttaccat tttttcaatt aagtcgagtg gtaatggttt gcctaatggc aattgaagtg
      901 tggccttacc tgtaatttta taatttttat acaaaccatc taattcctta tttcctgttg
      961 ggattggata aataccaata tgatttttat acccggcaaa ataaattata tactttcctt
     1021 gataatgata ggaaggcatt ccataactta ttttttcctc tacgtcggga ataatttttt
     1081 taattaaagt cctaatcttt tgtaaatatt tttgtgcatc tttcgggaaa ctattaatat
     1141 aggcattaac agattccggt tttgttttat cttttacact ggtggctttt gttgtttgta
     1201 ttttgaatgt caaattgttt gcggttaact cctgcaataa aattttaaca gcagattttc
     1261 caattccatg taattcgagt aaatgcgctt ccgtgtattt actaagctgt tttaaattac
     1321 taataccttc ctgctctagt gctcttcggg caggtgcact gatttttgac aaaaaacctt
     1381 cggctggttt tttctttttt tcacaaacag ggcaagtcaa acaatcgctg cttttataaa
     1441 aaacatgacc gttgggacaa actttccgaa ttccgcttat attattcatc aaacaatatt
     1501 aaaacaaaat atttattcag cgaaataagg ggcatttttt aagctttaat atataatttc
     1561 cgctgtctgt tttttaagtc ctttctatta aaccatgctt cataccacat ttcactatta
     1621 tcgctcaaat catctaaaaa cattggctta tccgaaagtt cattatttct ccaatggaaa
     1681 tgatccacca aacgaagtgc ttcaatggca aatgcagtta caatatcatc gtcatgaatt
     1741 tccaaaagat tatcgccatt tttttgttcc ggagtaaatg ataaattgct gctaccgcaa
     1801 taaacggtag gatctttacc tttaaaattc actacaataa atttatggtg aatggcatac
     1861 ccgtcaattc cggctacagt gccaaaaggt ggtggtaacg tattcgaaat tccttttgca
     1921 gccaccctga cacctctcag agcattgggt ttgtataaga acacgctgta ttcagcatta
     1981 tctccaattg aatcggtaat accataagta aatatctttt catttctcac ctgctcacga
     2041 actgcatcta aaattgcgct ggcattggta tctttcatga tagcaaataa tacatctgta
     2101 ttagaagcca cggaaattct ttttcttatt cggtcaaaaa ttgtttgtgc agcatcttta
     2161 gtatgaggag caaatgttat ggtcatttta ggtgttgaat ttcctgaaaa agaaaattca
     2221 ccagttgacc aagatgagcc ataaaaactt ttcatgtttg tttcaccaaa agatgcgtca
     2281 aatacatctt gatataattt tgcgattgta ttgtcgctaa aaattaatac gtgattacta
     2341 ttgatgtata atccgtttgt agaaaaatta gtagacccgg tgagcacctt aatcgcctta
     2401 tcatttttta tttgtataaa tactttacta tgtgccaatg ctttaaactt ccctctgtaa
     2461 atagcgttat tatcaggagc tacatattta aatttgcgtt caaactgtgt ttccattgat
     2521 tgggaatcac catgtgcacc ggcattatct aaaattattt gtaatcttcc ctgtttgccc
     2581 aattccaata gtcttttaca aatttcaggt tcatttaaat cgtacacaaa ggcttttaca
     2641 agtatagtag tgtctgcaac tgcttcatct aaaaatgtca aaattctttc tcttgcctgc
     2701 caacctaacc atttatgttg ttcttcaaaa gtataaggta ccgtttcata ctgtcgttta
     2761 gtgtcattcc atctttttgc tgtatctgca acctgtttaa catcaaacaa cagttcatct
     2821 tttgtttttg gaggtctaac cctgttattt tttacattaa atcgttttgc atacgcaacc
     2881 gatgaaacaa agcctcgggt aaatccaagt tccgtatttt tcactttgaa tggacctaca
     2941 taaattgtaa cagctacagt cagctcagta tttaatggca ataaaacgcc atttttgata
     3001 tatctgggtg taatttcata agtataattt ccaaaaattg gtaatgatgt attaatatta
     3061 gtattcggga catgcaccca attcattttt tgtattggag catataacgt cgaatttttc
     3121 tctgtaaatg gcacttggtt tatctggtaa aaggaatcag gaaatatcat acggttatat
     3181 agaaaggcat gttgcaaatt gccattaact tcaaagctat atcgaattgt aaaccctgcc
     3241 agattttttc taagtgaata ttgcaaatca aaggccagga gagtcatagc atcaccttgg
     3301 tacgcgcgaa cgctaacacc atttttttta ttaaatgttt ccatactaga tagcttagtg
     3361 ttacaatcaa taagcagtgg tgtctgctga atacagatac ttattgaaca gtgaaaacca
     3421 aatttaaaag aaactcaatt caaaaacaag tgtatttatc aaattcacgg ttttccgtta
     3481 atccaaatgg caatttggaa atattttaaa caaaaggtcc tttaagtgaa atcaatttca
     3541 tgtaatattg agttgatata tatgtaaacg ctcattttca acatcaaccg tagcagagaa
     3601 ttggaacccc agttttaata gcaatcttat tgatttcacg ttttcaggta aaagggttgc
     3661 taaaatagtt ttttcatgta ttttgtttat cacaaaatca agaacaccgc tagcggcttc
     3721 aaaagccaat ccttgttgct gatattccgg taaaaatgca aaacctaaat catagttatc
     3781 caaataagct cttttcatat aagaaatgat gccgacagga agttgattat cattgagcct
     3841 tgccacccaa taaacgaggt ttggtgtttg ctgaatttta tgaatatacg cctttgcatc
     3901 ttcttctgaa tgtacattcc tgtcacctat aaattgtatc catccctccg aatttactaa
     3961 ttgtaaaaca aattcgtggt cgtcaacggc aattggttgc agtaccattc tggaagtagt
     4021 gacaggtgtt gcaatattta tcatttggta attaatcagc aaatattaaa aaaatatggc
     4081 aattaaattg taattctgat aattaccata ttcaaacgtt gtttattagt gctttgtaac
     4141 gtttagaata tttgtaatgg catcctttgt tgaaacaaat tcatcaattt tcccataatt
     4201 actgttgaca gcatacacca ctgtagtgct atcctcagga aaatacatca tgttcgcata
     4261 ataaccaatt gcatctccgc tatgaaagta acattctccc ataggggtta ccattttaaa
     4321 aatgcctaac ccgtatgcaa tatcatagaa ctcagggtcc aaatttttag gagtttgcca
     4381 ggaaaacatt gcatccaacg aagttgaatt aataatttgc ccctgcatca atgcgcgcaa
     4441 aaagacattc aagtcatagg cagttgaaat aagacctcca tctgctgtat aataatccca
     4501 accgctgtaa taggtacttt caattacctg taaattgcta taaatatcaa tatacccgcg
     4561 aacaatacca tccggaaccg gatttttgcc tgcgaattga gtgctgctta accctaaggg
     4621 ttggaaaatt ttttcatcaa atacctgata caaaggtttg ccctcaattt gttcaatcaa
     4681 taaacctaat aaaatgtaat tggtattgga gtaagtcaca tcatcgccag gtgaaaaata
     4741 agccggtttg ttataagcat attgtaataa ttcttctgcc tcccagggtt tcaataaatc
     4801 gtttaacgat gctgtttgaa attgtaaatt atgaatataa ttaaatatgc cgcttgaatg
     4861 atgcatcagt tgttcaattg ttgcagttga tgcattttct attttatcca tgtaaatccc
     4921 atcaaggtaa ttggatattt tatcattgat attgagttta ccctcctctt gcaataacaa
     4981 tactgttgtg gcagtaaaca ttttaactgt tgaaccaact cgtgtgatat tacatggctg
     5041 catatctaca tcattaaata aatccgcttt accacttgca ccaacccaca taccatcagt
     5101 attattataa acagccattt caataccaac cacaccgttt gcagtgatgt tgtctaacaa
     5161 attttgatag gggccgtcat ttatattgtt atcactggaa tctatatacc caaaaccaca
     5221 tgtataaaat acaggttcaa ttacttctcc ttttctgcaa gctgcaaaca aaagacctaa
     5281 aactaaaatc agtagtatgt ttttcatttg cctgtgtttg agggtaaatt aaattttgca
     5341 ccaatatgca aattaatatg cgacatcagt ggaataaatc ccggtgaata aggatattca
     5401 atccaggatt gataacgaac aaatattttt ggtgatgtgg gttcgatttt gttaagttgg
     5461 taatttacat caaatgataa tgaaggggca attcgggcat tacctttatc tgttgcaata
     5521 gtgtaatgtc catcttcaaa aacatattct ttagtagttg ctaaagtgtg caaatagcct
     5581 actccaatta aagcaccaaa agaaaaacgg gaatttgtca aatattcgcc gcctaattcg
     5641 gtgtttacac taataccatg gtttaactga gcatgaaagt agtaactcag gttaactgtt
     5701 tggaataatc gaaaatgttc actttcctta taggtaaatt cagtcccgat ttgaataccc
     5761 ggatgtattg gagttgtaac taatttagta aatggaatag ccgtagcctc gttgtataat
     5821 gaaatttgaa tggggatatt tttttgttgt gcatgcagtg acacagcaac aaaaaagaac
     5881 atagggtgta gtaacagttt cacttaaaat aaataattga aaggtcaaaa ttaccggata
     5941 taatttgata atttggtcat attaacctca tttaaccaaa ttggatatac atttacacat
     6001 gttggtcagg ccctttagat ttacttacac caacaattaa caaccacaaa caaattccta
     6061 tctcccctat tgaagccggt aacattacaa aagtggaaat acctgaactg ttatattttg
     6121 gagccaaaag cgaagcagta aaatctatca gataacacat acaacccacc attaataata
     6181 ttcccaacaa ttttggcaga aatccgcttt tataaactaa ccaaccaaac gggaataacc
     6241 acaggcccca aaaaactgat aataaattaa tgccgttgtt gaaattttgc agcgatatta
     6301 atagttgttg ctgcagttga ttgattggaa tacctgcaaa aagatgttca ttattgagca
     6361 tggataaaac cgcaaattta tattgtaaat taacaaaagc aattggaata ccaatcaaca
     6421 ccaaaataac catactcttt gccatattag gatgaatgtt ttttaataac ttatacaagg
     6481 caaaaggcaa tatcataaaa gccagataac aaattacatt tcccaaaatg cccaacctaa
     6541 aaagcagttc atgagattgg atattttggt aagtaacagc tgcgttgttt aaatcgatta
     6601 attgatttgg cacatacatg agactgaata tcccacttat tacaacaatt aaataaatta
     6661 atcctgcctt acgggcaata cttttatttg tattcataat attcaggatt gatttttagg
     6721 ccagacaaaa gcaactcgaa taatcagcag cgtaataacc atttcgatga taccaagcaa
     6781 aaagtaaaat cgataccatt cgcttgtcgc accaatcaca atgctgaaca taattaatgt
     6841 aaaaattaat ccaaaaatca tattgaggat gcggttaatg gcaggtcgca aaaaaacaga
     6901 tacaattacc ataagtgccg gaatggccaa cataaccgtt gccaacaata aattcaatgg
     6961 ggtgttcaac agattgttgc cactaatcaa accttccact ttgcccggtg tatataattc
     7021 aaagtagtcg ccatacaaat aacaaaaggt gacggcagtc cacaaaaaag caagtttgat
     7081 tttagtattc acttcatagt ccttgaattt ggaccggata ggttgatttt cgggcatagt
     7141 ttgggtattt agggatagga cgaatgtggg tggagaaaag ttacattttt accacaatat
     7201 gtttaaaagc attaacacag ctaattactg agtaattaaa gcatgtaatt cagaatcagc
     7261 attatccatc tgcgcgggaa ttgccccata aaatatatgc tgaatagtgt taacattatc
     7321 tctcttaaat tcggaaaaaa aatagtcctc ttcatcaatg taaaattgcc tttcatcaat
     7381 tttccctgtg aatgatttag atatatatat catgttccca atctttactc tgacacgaag
     7441 tttagttgtg aattcacctt cataccttgg cattaatatg gtaacaaatt cctgtggatg
     7501 aattttcact ccccaatatc caaaaccaca aaagtcaaaa cctcttccct ctattggtcg
     7561 ccaatgacca gcagtatcaa ccgcttcctg aatagcaaaa acgtggctat ctttccctat
     7621 tataaactta acatttggtg tttgattaac aatataaaca ggataataat ttttcaattg
     7681 ttcatgcagt aaatcatccc taattattgt tgaagaataa tcaggaaata attctaaacc
     7741 atcataacca aaagtgtcgg ctcgccaaac gtccctaccg gaaacataat cgcttttttc
     7801 caagctgtct ttatgccgag aaatattaat atcaagcgtg tcacaaaact ttctctttcc
     7861 ttcataacga ttgtgagcat ctgatataga tattgcttta aaataaccaa ttgtatcatc
     7921 cgtttttttt tgaattacaa ctggaatttt aaaattagtt ttatttgcat ttactggaaa
     7981 atcttcctta caagacgaca atacaataac aaataggcaa attaatcctt ggagattttt
     8041 ttggtggttc ataacaaaaa aattaggtaa atgtgtttgt cccaagacta acgaattcat
     8101 ctcccatttc agtatcttac aaggatttgc taattacaat tccggcacaa tatctagttt
     8161 cggctaaaat taccgattga tgtcttattg taacaattac aataagtatc taatttatca
     8221 cacaggaaaa gtcaccaacg ccttccgcac actttcacgg aattcgtatt tatttattcc
     8281 catacttacc ttttggtgat cgaagtattg aataatattt tcggttggca tattgccggt
     8341 gaggtcgtcg gcagccatag gacatccgcc aaatcctttt attgcgccat caaatctggt
     8401 gcaaccgctt ttgtaggcag catcaatttt ttctaaccag gcatcgggag tagtgtgtaa
     8461 atgtgcacca aaatcaacac cgggaaattt aggtatcaaa tgggaaaaca agtaagtaat
     8521 actttcagga ttggcaacac caatagtatc ggctaaggaa acatatttga tgtccattgt
     8581 cactaatttc tccacccact ctgcagcaac ttcagcactc catgggtcgc catacggatt
     8641 accaaaaccc attgaaatgt aaatcatgag tttttttccg ttatcgtcac acaattgttt
     8701 tatttccttt acacgttcta cactttcggt aatacttgaa ttgatattac gttgctggaa
     8761 agtttcagaa attgaaaatg gaaatccgag ataagtaatt tgtttatgtt taactgcatc
     8821 ttctgcgccg cgaacattac caattatggc taataattta gagttggttt cactcaggtt
     8881 taattgagca agcacatccg ctgtatctgc catttgggga attgcttttg gagaaacaaa
     8941 actgccaaaa tcgatagtat caaatcccac cttcaacagc tgattaatat acgctacttt
     9001 tttatccgta ggaataaaat cgtgtaaacc ctgcatggca tcacgtgggc actctacgag
     9061 gcgtattgct tgcttttttg ctgtatcctg ggttaccatt gctttattga ttaaaattaa
     9121 ttactttttc ttttcataat cataaattac cggctttggt tccggtattt tcaaatcctc
     9181 attaaaattc gggaaaaaat aggcttttaa attgccatcc aactgtttag tatctaaaaa
     9241 ataccaggac acaccattgt tatcggaaac agccagtaaa gggctctcgt tcatcatact
     9301 tcctcccgga atagtcatgt atacaaattg tgatatgata gcatgtaact cggtacctgc
     9361 attataaatt ggacctggtt cacctatatt ggctgaatct actatataac cggctaattc
     9421 caattcctgc atcgattgct gaataatttc cagcatagtt gctttgcctc cacttaattc
     9481 aaatatttta ggataagtaa aatctaacaa cttttcataa ttatggtcct cgaatgcatc
     9541 cagcatggtg gttgcctggt ttttaatggt ttgttgcatt accacctgac tttgaccaaa
     9601 agataaaatg ctgatgctaa ctaaatagca actaaaaatt attctcacgt tattcccaaa
     9661 gggatattta ttgattaaaa aatacttaat taaagcacaa catttacaat acgtccttta
     9721 acgattatta cttttttagg cgtattccct tcggtccact taaccacaga ttctaatcct
     9781 aatactgttt ccctgatttc gttttcttcc atatctaaag gcaattcaat attcgctctt
     9841 actttaccat ttatggaaac cggataatta aaattacttt cagttaaata ggttgcatca
     9901 tatttcggcc aactttgtat atgcacagca tctgtatgcc cacatttttc ccaaagttct
     9961 tctgtaataa aaggcgcgaa aggagcaact aaaattaata attgttcaag tatttcgcgt
    10021 ttgtaacatt ttgcactttg taactcattt acgcaaatca taaaagcact tactgccgta
    10081 ttaaagctca gtttttcaat gtcttctcca gcctttttaa ttgttttatg caacaatttt
    10141 aattctgcag ttgtaggtgc ctcattggta attctcaatt gcccctgctc atcaataaac
    10201 aaacgccaca acttgcgcaa aaatccctgc acacctgtaa ttccttttgt atcccaaggt
    10261 ttacctgcat ctatagggcc taaaaacatt tcatacatgc ggaaacaatc tgcgccgtat
    10321 tcgttaatta catcatcagg atttacaaca ttatattttg atttagacat tttttctgtt
    10381 attgaagatg aagcaataaa gttaatagtt tgcatctctt ccaaaattcc aaaactacct
    10441 tgataatcag taaaatc
//
LOCUS       DAORKW010000061        10409 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_132_length_10409_cov_5.669210, whole genome shotgun sequence.
ACCESSION   DAORKW010000061 DAORKW010000000
VERSION     DAORKW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10409
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132_length_10409_cov_5.669210"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="PLB46_04155"
     CDS             complement(<1..1587)
                     /locus_tag="PLB46_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75740.1"
                     /translation="MSSIHKPAYQHLVEFYQTKLSHEFHYNYKEIHNAINEIIETKNF
                     SSEFKRDYSNFKEYVQGQKKSPNVKYVNFLLEIKDDVHLHLMINNPQIHFPLQDKFYK
                     LFNSKLKLILNNEKKEFQHDTTFFSRRYKIHNNDVGEFLTAFPTTIWAIIDKESYDQD
                     YVSDLILNSSDSASIQSVKKMSTFEPNQIILGHALSPVSDSIFLLMELGRYLRICHTL
                     NKSPRIYLTDTSWAKYNYSVIEYFSDDPEKAKTHLNANFRFRKKLYGMLGIECEIQDP
                     SNFLSSGEEIKQLEKTIDSYNTFGNLLFNSISNGERGVENKKRVLDFIDKSLDKKIDE
                     RPEDEYNNELTFLKLYPAIPLEVIDKMFFITKTVLKNYKDINEHTLKYAFYQRFAQRK
                     YSGCLKVGVRSETKFDQSFARMDKYENEHAKSNVNNKLYGVYLDHYFFSRLENNQPID
                     IIPYTFPSGRFSSAFPKIEDAINNAILLWDFIDEDRKSKVKRLIDNLELAQLAIQFSD
                     LLSFTFYFFKTNVTYWVGSEI"
     gene            1839..2069
                     /locus_tag="PLB46_04160"
     CDS             1839..2069
                     /locus_tag="PLB46_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75741.1"
                     /translation="MKRLNKNAAHKKASQSIEKLNSIPDSSIISITQDILKSSRLSDT
                     HQFVLKFQSFEYPVELEEGNVQCSVNDTENPN"
     gene            2231..2413
                     /locus_tag="PLB46_04165"
     CDS             2231..2413
                     /locus_tag="PLB46_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75742.1"
                     /translation="MEEIKKSVDTFVIERESTEPQNEWWESIELHLYFELAAILSKSE
                     WDEIQSLALRSSYKIS"
     gene            2595..3722
                     /locus_tag="PLB46_04170"
     CDS             2595..3722
                     /locus_tag="PLB46_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75743.1"
                     /translation="MSLTDCTCLSSLLSPAGSSLIPQFLTSFTIFQNFKFLFMNLFKF
                     TYGIRDKVLERRLETKITRMEDNAKQDTGIAVSQNLTNPQVILTEVAAETDVLKEEVT
                     KTLNPEIGKLTEEIEQTDLEALQSVAKGQEEQLNHKISALEVDIKRTTLDFDFKKLIW
                     TMVAIVALCSIDAGANYSSFQVIAKNLLGAIMLAIAVAIALSSSAHTIGMKIRTASST
                     LMKRLWFFGGLAGAGVVFYFLGMIRQEYTGKADGFANSPLLWMCFNLFFFAVALLLAA
                     TKFPTKEQRLAYVALQDKKKQLASLKNEKETLLNALRKEESRINECRQKLEAFLTYRA
                     ELLSSLDKEKERINAMCLKEFELKGGKIATKVSFTSKNNKS"
     gene            3719..4438
                     /locus_tag="PLB46_04175"
     CDS             3719..4438
                     /locus_tag="PLB46_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75744.1"
                     /translation="MRVILFTYTLPAILAVCLSACEAEPITQDISILLDQTEEHFTHV
                     SVEDIAKKSVITESITNGESIRILSITELGFNAVSVFRMDVVTNVLLDNDFERDSLVV
                     TYFTDIDSALSGLLLERRERTGSVICKILAGELNRLSASSVDRKLLIINSDLMEKSFI
                     DFYSTTIFNQIKNEPAKIMDTLIKRYPLKSLQGIEIYIVYKPTDKSDSERFEIISGFY
                     ENFLESLGAKVFVGSDLNTQN"
     gene            4471..4629
                     /locus_tag="PLB46_04180"
     CDS             4471..4629
                     /locus_tag="PLB46_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75745.1"
                     /translation="MARPLGNTLFDAIKQLTIALFKLIAIAFAWLCKIAGMVLLKAGE
                     TIEKMISR"
     gene            4626..6062
                     /locus_tag="PLB46_04185"
     CDS             4626..6062
                     /locus_tag="PLB46_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRF75746.1"
                     /translation="MIKLFAAIFELLFSVVAEIVTTIFDFIVSIIQPKRKTEYTADFI
                     APGSILRKHDTGFCLTGKFSMSSEDSHKNCLVLGGSGSGKSSIILINSMLFMMHGKNS
                     LIINDPSGELRLLTSGALIKYGYIVKVLDYNNILSECFNPLKRCKSISDIQKLSALLV
                     RNALGESKGDQFFNKIAESLLSLFIRYLVFYVKPEYSTLYNVLHLINLFAGGGAEKID
                     RLIVKTKDEKLLAEYKAFIAYGDKTLMSIVATVKSALSLWSDETIAKITSIDTIDFAE
                     FRVKRIALFINNSVPTMNYYGALSSLFFQQFFNEILVRIPDKKENSVFFLLDEAGSMY
                     LPSISVTISNIRKNRGGILCIYQDYSILENIYGAYEAKNISANCYAKVYLPGQPIETC
                     RMLETTLGKFEYEDEKSGNTKLRSLMTADEIRMTDKAIILIGNKAPIHATLHPFYKNS
                     KLKSLTELPSYEPNNILPFDTPPLIAID"
     gene            6063..6602
                     /locus_tag="PLB46_04190"
     CDS             6063..6602
                     /locus_tag="PLB46_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75747.1"
                     /translation="MTRKAKIGSNVNSYLRATGILEHGTHDQIQAARKKYWSEYKRKW
                     RNEKRKKEKEFTISLDAEQQKIIAQVSKSHKLSRTAFMKSAIFGYINTTFIVPDSHEV
                     KKISQLLAMTYNSIQELFEENGIDTLSVKELLERLAELEREVLPKLHNPKSLEEYIIE
                     YICKADGNKEQLIDFINAI"
     gene            6605..7522
                     /locus_tag="PLB46_04195"
     CDS             6605..7522
                     /locus_tag="PLB46_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HRF75748.1"
                     /translation="MIVKSLTRRSNTLQLVNYLFKQEKGERLQPILKRNLRSRTAEGW
                     AKEIDANDALRIRRRTDNIRLYHTVLSFSDRDKAHIDKSLLKDISKKYVELRGRDSIF
                     LASSHTDKDHIHLHIIQSGTKYLTGESNRISKKEFHELKLALDLYQKEKYPQLIHSLP
                     EHGKSLRMQKTEREYQSQNRGGILSQKEQLFQTMETAYTKSKSLGHFLDQLKSEGYNS
                     YNRGGKLYGLEDTEGRHYRFKTLDYAEKIKSLQKEADIEEKALQDLADLRESKNMERE
                     EEYEREMEEEETVEEETETDDSDPNYTSE"
     gene            complement(7503..8138)
                     /locus_tag="PLB46_04200"
     CDS             complement(7503..8138)
                     /locus_tag="PLB46_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75749.1"
                     /translation="MTIEELVQQAKSIRDSDISDVNSLLSESLEFLRMYAGEKTAFYK
                     QLLERSKKIPIDTEWNYNPKEIPRLVNGALDAFIRYAEKGLLNGVTIQRQAEIDVVSD
                     ILSQAKMLLETNGVHPASPAVVAGAALEEFLRNWLEEQGLKSAHNKPTLDGYSKILYE
                     KELIDKQDLKDIIAWAELRNNAAHGLWDKVNNKEKVQLMVESINLFIRKYS"
     gene            8347..8538
                     /locus_tag="PLB46_04205"
     CDS             8347..8538
                     /locus_tag="PLB46_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75750.1"
                     /translation="MLSFETCKKILSSHGRTYSDDEIKIIMGVLQQLAYFEYTFLKNK
                     KVMHGKGSDLYQGFNRRTG"
     gene            complement(9366..9494)
                     /locus_tag="PLB46_04210"
     CDS             complement(9366..9494)
                     /locus_tag="PLB46_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75751.1"
                     /translation="MKKNPAIRQMNDAQHPLLPFERTLVDSGNKNICPRYPLMRFL"
     gene            complement(9999..10070)
                     /locus_tag="PLB46_04215"
     tRNA            complement(9999..10070)
                     /locus_tag="PLB46_04215"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10035..10037),aa:Glu,seq:ttc)
     gene            10169..>10409
                     /locus_tag="PLB46_04220"
     CDS             10169..>10409
                     /locus_tag="PLB46_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HRF75752.1"
                     /translation="MSNYRVAINGFGRIGRIFLRRALEKNNFEIIAINDLTDVKTLAH
                     LFKYDSVHGRFSGTVEVSGNDLLVNGKLIRVYAEKD"
BASE COUNT         3546 a         1747 c         1909 g         3207 t
ORIGIN      
        1 aatttccgat ccaacccagt atgtcacgtt tgtcttaaag aaataaaatg taaaactaag
       61 taagtcggag aattgaatag ccaattgagc gagttctaaa ttgtcaatta atcttttgac
      121 tttacttttt ctatcttcat cgatgaaatc ccataagagt atagcattat taattgcgtc
      181 ttctatttta ggaaaggctg atgaaaaacg gccactagga aacgtatatg gtataatatc
      241 aatcggttga ttattttcaa gccttgaaaa aaagtaatga tcaagataga ccccatataa
      301 tttattattt acattagatt tggcatgttc gttttcatat ttatccattc tagcaaaaga
      361 ttgatcaaat ttggtttcac tccttacccc aacctttaaa cagccactgt attttcgctg
      421 agcaaatctc tggtaaaagg catatttcaa tgtatgttca ttaatatcct tataattctt
      481 gaggaccgtt ttggtaataa aaaacatttt atcaattacc tccaatggta tggcgggata
      541 tagctttaaa aaggtcagtt cgttattata ctcatcttct gggcgctcat caattttttt
      601 atccaaggat ttgtcaataa aatccagcac tctttttttg ttttctaccc ccctttcacc
      661 gttactgata gaattgaaaa gaaggtttcc aaaagtgttg taagaatcaa tcgttttttc
      721 aagttgtttg atttcctccc cagaagaaag aaaattgctg ggatcttgaa tttcacattc
      781 gatgccaagc atcccatata gtttttttct aaatctgaaa ttggcattta ggtgggtttt
      841 tgctttttct gggtcgtccg aaaaatattc aattacactg tagttgtatt tggcccaact
      901 tgtatcagtt aaataaattc gtggtgattt atttaatgta tgacatattc ttaagtatct
      961 tcctagctcc attagcaaaa aaatgctatc acttaccgga ctcaaagcgt gacctaaaat
     1021 tatctgattc ggttcaaaag tggacatttt ttttacagat tgtattgagg cgctatcaga
     1081 gctatttaaa attaagtcag atacataatc ttgatcataa ctttctttat caattatggc
     1141 ccaaattgta gtgggaaagg cggtaagaaa ctctcctaca tcgttattgt gaattttata
     1201 cctcctgcta aaaaaggtag tatcatgttg gaactctttc ttttcattat tcagaataag
     1261 tttgagcttc gaattgaaca atttgtagaa cttatcttga agcgggaaat gaatttgagg
     1321 gttattaatc ataagatgta agtgaacatc atctttaatt tctaataaga agttgacata
     1381 cttaacattt gggctctttt tttgcccctg gacatactct ttaaaattgg agtagtctct
     1441 tttgaactct gaactaaagt tctttgtttc aataatttca ttgatagcgt tatgtatttc
     1501 tttataattg taatgaaatt catggctaag ctttgtttga taaaattcta ccaaatgttg
     1561 ataggcaggc ttgtggatgg agctcattat gtattatttt ataccaaatt taagtatagc
     1621 gcactacaaa tgacaaataa agtgtcttat ttattaccaa taactcactt tttgtaaaag
     1681 gtaagactat gacttgtgcc atatgcgtgt ttgtggagat atcattctaa atcctttctc
     1741 gcctttaata aagagtaagt tatgagtata tgatgtaata tcaaaaaaat acttctaata
     1801 taccataaga agtattttcg tagtaaatct ctgacaaaat gaaacgacta aacaaaaacg
     1861 ctgctcacaa aaaagcttca cagagcatag aaaaattaaa tagtattcca gacagcagca
     1921 taatatctat aacacaagat attcttaaga gttcaagact atctgatact catcaatttg
     1981 ttcttaaatt tcaaagtttt gaatacccag tagaattaga ggaaggaaat gtgcaatgct
     2041 cagttaacga tactgaaaac cctaattaat aggattagac tcaacacaag gtatttaaac
     2101 cgattttaat tttataatta atgataccta atacttataa tggttttaaa aattactacg
     2161 caacaagtgc tcacagggtt tcattttatc tactaaatga atattatctc acaatataaa
     2221 atcaactcat atggaagaaa tcaaaaaaag tgttgatacc tttgttattg aaagagaatc
     2281 aactgaacct caaaatgaat ggtgggagtc tatcgaactt catttatatt ttgagcttgc
     2341 tgcaatctta agtaaatctg aatgggatga gattcaatct cttgctttaa ggagtagtta
     2401 taaaattagt tgaactttat atatttttcg gataactcct tttgatttaa gaaccatttc
     2461 attctgtctt aaaatacata aaactaagtg agctgatgtg tgcacattga ccttactaca
     2521 ttataaaata atcaataaaa gtaagattaa aataacttaa ttgtttgctc tttacggtgg
     2581 gtttatttct tgtcgtgagc ctcactgact gcacctgcct ttcttctctc cttagccctg
     2641 cggggtcatc cttgatcccg cagtttttaa cttctttcac catttttcaa aactttaaat
     2701 ttttatttat gaatcttttc aaatttactt atggcatccg ggacaaggtc ctagagcgtc
     2761 gtcttgaaac taaaatcacc cgtatggagg acaatgcaaa acaggacacg ggcattgcag
     2821 tttctcaaaa tttaaccaat ccacaagtta ttttaactga agtagctgct gaaactgatg
     2881 tgctaaaaga ggaggttacc aaaaccctca atccggaaat tggaaagctc actgaagaaa
     2941 ttgaacaaac agacttggaa gcattgcaaa gtgtggcaaa aggtcaggag gagcaattaa
     3001 atcataagat ttctgctctt gaagtagaca tcaaacgaac tacacttgat tttgatttca
     3061 agaaattaat ttggactatg gttgccattg ttgcattgtg ttccattgat gcaggtgcaa
     3121 attactccag ttttcaggtt attgccaaaa accttttagg tgcaattatg ctagctattg
     3181 ctgttgcaat cgctttgtct tcttcagcgc atacaatcgg catgaaaata cggactgctt
     3241 cctctactct tatgaaaagg ctatggtttt ttggtggctt agccggagca ggtgttgtgt
     3301 tttacttttt aggtatgata cgccaggaat acaccggtaa ggctgatgga tttgcaaact
     3361 caccgctatt atggatgtgt tttaatttat tcttttttgc tgttgccctt ctgcttgctg
     3421 ctacaaaatt tccaactaaa gaacaaagat tggcatatgt ggcattacaa gacaagaaaa
     3481 aacagcttgc ttcactaaag aatgaaaagg aaacgctatt aaatgctttg aggaaggaag
     3541 aatctagaat taacgaatgc aggcaaaaac ttgaagcatt cctcacttac agagctgaac
     3601 tattgtcctc gctcgataaa gaaaaggaac gcatcaatgc aatgtgtctg aaggagtttg
     3661 aattaaaagg gggtaagata gccaccaaag tatcattcac atctaaaaat aataaatcat
     3721 gagagtaata ctattcacct acacactgcc ggcaatcctg gcagtgtgcc tttcagcttg
     3781 cgaagctgaa cccattactc aggatatatc tattttactt gaccaaacgg aagaacattt
     3841 tacacatgtt tcggtagaag atatcgccaa gaaatctgtt ataaccgaaa gcataaccaa
     3901 cggagaaagt ataaggatac tatcgattac tgaacttgga tttaatgccg tatctgtttt
     3961 tagaatggat gtagttacca atgtattgtt agataatgat ttcgaacggg acagtttggt
     4021 agttacatat tttactgata ttgactcagc gttatctgga ttgttgctgg aaagacgtga
     4081 acgaaccggt tcagtaatat gcaaaatttt ggctggagaa ttaaacaggt tgagtgcatc
     4141 aagtgtagat cgaaaactac tgattattaa ttcagatttg atggaaaaat catttattga
     4201 tttttattca acaactatct tcaatcagat caaaaatgaa cctgctaaaa taatggacac
     4261 tttgataaag cgttatccat tgaaaagctt acaagggatt gaaatatata ttgtatataa
     4321 accgacagat aaatctgata gtgaacggtt tgagataata tctggcttct acgaaaattt
     4381 ccttgaatct ctcggcgcaa aagtgtttgt gggcagtgat ttgaatacac aaaactagca
     4441 ttcacaataa ttacttaaaa cttcaagaag atggcacggc ctttaggtaa cacattgttc
     4501 gatgcaataa agcaactcac tattgctttg tttaaattaa tagcaattgc ctttgcatgg
     4561 ctttgcaaga ttgccggtat ggttctttta aaagctggcg aaacaattga aaaaatgatt
     4621 tctagatgat aaaacttttt gcagcaattt ttgagctatt gttttcagtt gtagctgaaa
     4681 tagtcactac catattcgac tttattgtct caattattca gccaaagcgc aaaactgaat
     4741 atactgcgga ttttatcgct ccaggttcca ttctacgaaa gcatgatact ggtttttgcc
     4801 tgactggcaa attttctatg tcgagtgaag acagtcataa aaattgtctc gtgctcggag
     4861 ggtctggatc gggaaagagc tcaattattt taattaattc catgttattc atgatgcatg
     4921 gaaaaaactc tttaattatc aatgacccat ctggcgaatt gcgattattg acaagtggtg
     4981 cattaataaa atacggatat attgtaaaag tgctggacta taacaatata ctttccgaat
     5041 gttttaatcc tttaaaacgt tgtaagagta tttcagacat tcagaaatta tctgcgcttc
     5101 ttgtccgaaa tgcacttggt gaatcaaaag gagatcagtt tttcaacaaa attgcagaga
     5161 gtttgctctc actttttatc aggtatttgg ttttttatgt aaagcctgaa tattccactt
     5221 tgtataatgt cttacattta attaatctat ttgcaggagg cggtgctgaa aaaattgacc
     5281 gcctaattgt aaagactaag gatgaaaaat tattagcaga atataaagct tttattgcgt
     5341 acggggacaa aactttgatg tcaatagtag caactgtcaa aagcgcccta tcactttggt
     5401 cagatgaaac gattgcaaaa attacctcta ttgatactat agattttgct gagtttagag
     5461 taaagagaat cgctttattt ataaataatt ctgttccaac aatgaattat tatggcgcac
     5521 tatcctcact attctttcaa cagtttttca atgaaatatt agtgagaatt ccagataaaa
     5581 aagaaaacag tgttttcttt ttactggatg aagcgggctc gatgtatttg ccttcgatat
     5641 cagtaacaat ttcgaatatc aggaaaaata ggggtggtat attatgtatt tatcaggact
     5701 attccatcct tgaaaatatt tacggagcat atgaagcgaa aaatatttca gccaactgtt
     5761 atgcaaaagt atatttgcct ggtcagccga ttgaaacatg caggatgtta gaaacaacat
     5821 tggggaaatt tgaatatgaa gatgaaaagt caggtaatac aaagttgaga agcttaatga
     5881 cggctgatga gattagaatg actgacaagg ctattattct cataggcaac aaagctccga
     5941 tacatgctac gctccatccg ttttataaaa actccaaatt aaagagtctc actgaattac
     6001 cttcgtatga gccaaataat atattgccat ttgatactcc tccattaatt gcaatagatt
     6061 aaatgacgcg caaggctaaa ataggaagta atgtaaatag ctatcttagg gctacaggta
     6121 tactagaaca tgggacacat gatcagatac aagcagcacg gaaaaagtat tggagcgaat
     6181 ataaacgaaa gtggaggaat gaaaaaagaa aaaaagaaaa agagtttact atctcacttg
     6241 atgctgaaca acaaaaaata attgcccaag tttccaagag tcataaactg agccgaactg
     6301 catttatgaa aagtgcaata tttggctata tcaatactac atttattgtt cccgatagtc
     6361 atgaggtgaa aaaaatctcg cagctattgg caatgaccta taactcgata caggaattat
     6421 ttgaagaaaa tggaatagac acgttgagcg taaaggaatt acttgaaaga ttagcagagc
     6481 ttgagcgtga ggtactaccc aaattacaca atcctaaatc gttggaagaa tacatcatag
     6541 agtatatatg caaagctgat ggcaacaaag aacaactaat tgattttata aatgctatat
     6601 aaaagtgatt gtaaagagtc tgacacgaag aagtaacaca ttgcaattag tcaactacct
     6661 attcaaacaa gaaaaaggag aaagattaca acccatatta aaacgtaacc tacgttcaag
     6721 aacagcagaa gggtgggcta aagaaattga tgcaaatgat gctttacgaa ttcgaaggag
     6781 gactgacaac attcgtttat atcatacggt gctttcgttt tcagatagag ataaagcaca
     6841 tatcgataaa tctcttttaa aggatatttc caaaaaatat gtagaactgc gtggtagaga
     6901 tagtattttc cttgcttcat cacatactga taaagaccat attcatttac atatcataca
     6961 gtccgggact aaatatctta ccggtgagtc aaaccggatt tcaaaaaaag aatttcatga
     7021 gttaaaattg gctctcgatt tatatcaaaa agaaaaatat cctcaattaa ttcatagcct
     7081 gcctgaacat ggtaaatcac ttagaatgca gaaaactgaa agagagtatc agtctcaaaa
     7141 tcgtggaggt atattatcac agaaggaaca gctttttcaa actatggaaa ctgcatatac
     7201 aaaatcaaag tcgctaggtc attttctgga tcaactaaaa tcagaaggat ataattctta
     7261 taaccgaggt ggtaagttat atggattgga agacacagaa ggcagacatt acagatttaa
     7321 aacccttgac tatgcagaaa aaattaaatc cctgcaaaag gaagctgata tagaagaaaa
     7381 ggcattgcaa gacctggcag atttgcggga atcaaagaac atggaacgtg aagaagaata
     7441 cgagagagaa atggaagagg aggaaacagt cgaagaagaa accgaaactg atgatagtga
     7501 tcctaactat acttccgaat aaacaaatta atgctttcaa ccataagttg aactttctct
     7561 ttgttattca ccttgtccca taatccatgt gccgcgttat ttctcaattc cgcccaagca
     7621 ataatatctt ttaaatcttg tttgtcgatt agttctttct catatagaat ctttgaatag
     7681 ccatctaagg tcggcttgtt gtgtgcggat tttaaacctt gttcttctaa ccaatttcgt
     7741 aaaaattcct ccaatgcggc acctgcaact acagcaggtg aagctggatg aactccattt
     7801 gtttctaaaa gcatttttgc ttgagacaaa atatctgata cgacatctat ttccgcttgt
     7861 ctttgaatgg taactccgtt taataatccc ttttcggcat agcgaataaa tgcatctaat
     7921 gctccattta caagtcgagg aatctccttc gggttgtaat tccactctgt gtcaatcggt
     7981 attttctttg atctttcaag tagctgttta taaaacgcag ttttttctcc ggcatacatt
     8041 cgtaaaaact ctaagctttc cgaaagtaag ctgtttacat ccgatatatc tgaatctcga
     8101 attgattttg cttgttgcac taattcctca attgtcatat aagttgaatt tacatacaaa
     8161 attaataatt aaaaggggtg cgggtctccc gcccagattg cgtctccaaa aaggcagatt
     8221 aaaaatcgcc ctgcggagag gcaatctggc aactgctatt tcccttagaa tttatatgaa
     8281 ttttcaaaac attttgaaca ccaaattaaa ttgtatctct tgtaattaaa gcgcaaaatt
     8341 gttgaaatgt tgtctttcga aacatgcaaa aaaatactat cttcacatgg cagaacatac
     8401 tctgacgatg aaataaaaat cattatgggt gtgttacaac aacttgcata tttcgaatac
     8461 acatttctta aaaacaaaaa agtaatgcat ggaaaaggta gtgatttata ccagggtttc
     8521 aacagaagaa caggctgaca aaggatatag tttgcgcgac caaaaagacc gtcttgaaaa
     8581 gtattgtgcg attaaaaacc tcaccatagt aaaacattat gaagacgact attctgcaca
     8641 aacatttgaa cgacctcagt tcaaaaaatt tctcgaattt atcaagcaca acaaaagagc
     8701 agcagacggt tttttgatta ttcgttggga caggttcgca aggaatgcaa ctgatgcgct
     8761 ggttatgctc cgtaacctgt cacgacaagg agtggtggtt gatgctgtgg aacagcccat
     8821 cgacatgagc atccctgaaa ataagttcat gctgaacttt tatctaaccg ctccggaagt
     8881 agaaaatgac agacgtgcaa tgaacaccat caatggtatg cgcagggcta tgaaggaagg
     8941 cagatgggta agcaagccac cgcttggtta ccaatttatc tacgatgaaa aacaaaaacc
     9001 gctgcttgta aaaagtgaaa gagcattatt aataaaggaa gtatttgaat tgcttgcaac
     9061 cggttcacat acaatggagg aagtgagaaa ggaaataaaa ttgaagggct tgaaatgtga
     9121 aaagaataac ttttggaaat tagtaagaaa tccggtgtat tgcggcaaga tattactgaa
     9181 agcatacaaa tcggaacctg aagaagtttt acaaggcata catgaaccga ttgttagcga
     9241 agaattattt tatcgagttc aaaatgtgtt gttcaacaag aagaaaatca aggcgaagcc
     9301 tcaaaccaaa tcagattttc taccattgcg tgggttttta gtctgtcctg attgtggcaa
     9361 gaatcttaca ggaagcgcat caaagggtaa cgggggcaaa tatttttatt accactgtca
     9421 accagggtgc gctcaaaggg taaaagcgga tgttgcgcat cattcatttg gcgaatggct
     9481 ggattctttt tcatttaaac ctgagattgc agaactctat ttggctgtta ttgaagatac
     9541 ctttaaaatt gaggaaggcg acagaatcgc aactacaaga cgcattcaaa aggacattga
     9601 aaatgcaaat aacaatttat tgaaagttga aaaaaaatat atcaacgatg agttggagag
     9661 agattcatat ctccgactaa aagaatcata caaaaaagaa gtatttgaac tgcaagatcg
     9721 tttgatagat ttacaatcaa gcgatacaga atttatgcac tatatgagct atggtttttc
     9781 cgttttatct aacctcaaat catgttacga atctgctgat gtagaattaa aacaaaaatt
     9841 gattggttcg atatttcctg aaaaattaat ttatgcagat ggaaagtatc gaaccacaaa
     9901 agacagcgat atacttcggc tactttgctt gagtggtgcg gatttcaaag gaatggaaaa
     9961 taaaaagtcc cgcacaaatg cgggacagtt cgctaaggta gcccgtaggg gaatcgaacc
    10021 cctgtaccaa gaatgaaaat cttgtatcct aacccctaga tgaacgggcc gtttttgtaa
    10081 acgagagtgc aaaagtaata gaaaatttat ttttattaaa atttctacac aaaaattctc
    10141 tttttgaagc taattttaca gtcatattat gagtaactat cgcgtagcaa tcaatggatt
    10201 cgggcgtatc ggacgaattt tcctccgaag ggccttagaa aaaaacaact tcgaaattat
    10261 tgccataaac gaccttaccg acgtaaaaac actggctcat ttgtttaaat acgactctgt
    10321 tcatggtcgt tttagcggta ccgtggaggt ttcaggcaac gatttgttgg taaacggcaa
    10381 acttatcagg gtttatgccg aaaaagacc
//
LOCUS       DAORKW010000062        10360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_134_length_10360_cov_5.058224, whole genome shotgun sequence.
ACCESSION   DAORKW010000062 DAORKW010000000
VERSION     DAORKW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134_length_10360_cov_5.058224"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLB46_04225"
     CDS             <1..336
                     /locus_tag="PLB46_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75753.1"
                     /translation="ITTAFCTDTQCDTVVIAPTGIDEETIIGVLNAYPNPANDLINIT
                     LENAITANTSIVINDLSGKQLKEIFTGTLYSGQHNFQTNINVLPEGIYLIHVISDAGG
                     VHVIKFIKQ"
     gene            544..3912
                     /locus_tag="PLB46_04230"
     CDS             544..3912
                     /locus_tag="PLB46_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRF75754.1"
                     /translation="MTDVQPYQPKHKIRIVTAASLFDGHDAAINVMRRIIQATGVEVI
                     HLGHDRSVEEVVNCAIQEDVQAIAMTSYQGGHTEYFKYMYDLLKQKGCEHIKIFGGGG
                     GTILPSEIEELQNYGITRIYSPDDGRAMGLQGMINDLVQKSDFATGINLNGELTHLTK
                     ERDWKSIGKLISAVENNTPDASGIMTQVEVLIKNNNTPVLGITGTGGAGKSSLVDELV
                     RRFLIDFTDKTIAILSVDPSKRKTGGALLGDRIRMNAINNARVYMRSMATRQANLALS
                     KHVDEAVKILKAAQFDLIILETSGIGQSDTEITEHSNVSLYVMTPEYGAASQLEKIDM
                     LDFADLIAINKFDKRGAQDALRDVRKQFQRNHKLFDVETEKMPVFATIASQFNDPGTN
                     QLYRHLMDTIKEKAKVDLHSDFHITDEMSKKIFIIPPQRTRYLAEISENNRAYDKKVV
                     EQKTVAQNLYALHTSKAIVSDAELKNKLEEEYQRVAKKIDNENLDLIHSWNKKLASYK
                     DEYYIFKVRDKELKIKTHSESLSHLEIPKISLPKYEAWGDILQWQLQENVPGEFPFTA
                     GIYPFKREEEDPTRMFAGEGGPERTNKRFHYVSLGMPAKRLSTAFDSVTLYGNDPDYR
                     PDIYGKIGNSGVSICCLDDAKKLYSGFDLADPKTSVSMTINGPAPMLLGFFMNAAIDQ
                     QCEKYIIENKLEAAVETKIQEIYKGREHLRPKYNADSLPTGNNGLGLLLLGVTGDQVL
                     PADVYAVIKAKTLSTVRGTVQADILKEDQAQNTCIFSTEFALRLMGDVQEYFIQQKVR
                     NFYSVSISGYHIAEAGANPITQLAFTLANGFTYVEYYLSRGMNINDFGPNLSFFFSNG
                     IDPEYAVIGRVARRIWAKAMKLKYGADDRTQKLKYHIQTSGRSLHAQEIDFNDIRTTL
                     QALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGLAKNENPIQGSFI
                     IEELTNLVEEAVLSEFDRITERGGVLGAMETMYQRGKIQEESLFYETLKHTGEYPIIG
                     VNTFLNSKGSPTILPMEVIRATEEEKEAQITTLKNLHEKNAELTAKHLRHLQTTAIQN
                     NNLFEALMEACKFCSLGQITKSLFEVGGQYRRNM"
     gene            complement(4087..>5483)
                     /locus_tag="PLB46_04235"
     CDS             complement(4087..>5483)
                     /locus_tag="PLB46_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75755.1"
                     /translation="EINYGYNYDKASIFFNFGVTGAVAGEKTYYFDDVMFGEPVVVVN
                     YNVTFNVNMNEVVAGFTTPEVNGTFNGWCGGCNPLSDPDGDGIWSTTLSIPEGTYQFK
                     YAYDAWAGSEILADGAVCTINDGGFVNRVLEVSSDMVMDPVCWGSCLDCGAAVTYDVL
                     FTVDMNEVAAAFTTPEVNGTFNGWCGGCAPMSDLDGDNIWELTIPLEAGTYEYKFAYD
                     TWAGSETLLEGDPCTITAFGFTNRSLVVTAPVALDTVCWGSCTSCETAVPAHIVKFQV
                     DMSTVAAAFTTPEVNGTFNGWCGGCAPLSDLDGDNIWELDILLPEGTYEYKFAYDAWA
                     GQEELTSGDDCTITTDGFTNRALTVTGPATLDAVCWSKCEACDVSINDIVIGDVSVWP
                     QPAKGVAYLTFEDQIFINANAIITDITGRMIQQSTIVGNNHAFNLNNMVPGVYQVVVI
                     KNGQILYANSLSVQ"
     assembly_gap    5484..5583
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5584..6283)
                     /locus_tag="PLB46_04240"
     CDS             complement(<5584..6283)
                     /locus_tag="PLB46_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75756.1"
                     /translation="MKQIFTYLLLCTLATSAMAQTYNVTFKVNMNEVAAAYTTPEVNG
                     TFNGWCGGCNPLFDDDANGIWEVTIALAPGTYEYKFAYDAWAGQESLTEGSPCTVTAF
                     GFTNRTFTLGEADMVLNQVCWGSCDDCDGVVLAQMDLPVTFDEAGVDYGLIGFGGAEA
                     STIEVDPTDAGNMVAKVIKSGAAELWAGTTVTDGAGSGFATDIPFTVDATTMTVRVWS
                     PDAGIQVRLKVEDHL"
     gene            complement(6631..8091)
                     /locus_tag="PLB46_04245"
     CDS             complement(6631..8091)
                     /locus_tag="PLB46_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75757.1"
                     /translation="MKNLTLFFVLLGFSLFATAQTYNVTFKVNMNEVAEPFTTPEVNG
                     SFNGWCGGCAPMSDPDLDGIWEVTIALAPGTYEYKFAYDSWTGQENLVPGTFCTVTNF
                     GFTNRSLVVDAADIVMDPVCWASCDNCGEGALYDITFQVNMNGVAGPFTTPEVNGNFN
                     GWCGGCAPMSDVDGDGIWAITITLAPGTYEYKFAYDAWTGQEALTEGSPCTVTNFGFT
                     NRSLVVTDNATLDPVIWGTCDFINTTIAPAGTVTICSGTTQAFAAPTGYTGYQWKKNG
                     VNIPGATSSNYTANQAGNYSVVISQGTAFAQSAATTLVVSKPKPKIQPVGSLDICATG
                     SVLLKGKIGVGSTYQWYKDGVAIVGAPLNLVATSIGSYKLKETTPAGCTKSATVVVTT
                     SCKLEGANDDVFAIYPNPNNGDFTIQVQTASLTADAINIAIYSSTGAVVYQTTNTIDN
                     GNPNQTISLGNTLASGMYMVKINTGNTSVTKTLIVE"
     gene            8206..8931
                     /locus_tag="PLB46_04250"
     CDS             8206..8931
                     /locus_tag="PLB46_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRF75758.1"
                     /translation="MAKTNKTLRRRNEYQIYDVPIDSFFELAFSIDCVVFGYGKKDLQ
                     VLLIQRGAEPYKGLWALPGDLVYPDEDLDTSAGRVLKALTSLPDLNFEQVHTFGAVDR
                     HPLGRVVTVAYYAIVNMKGFNPKAAFWADKAKWHSVKNLPKLAFDHRDIIDYSYKKLK
                     ESTMTKPLWNGVIEEKFTLTQLQSFFETVLDKKFDKGNFRKKIDLFQYVKETEEFEKD
                     VPYRPSRLFKFDKKLYQKNEESS"
     gene            8915..10264
                     /locus_tag="PLB46_04255"
     CDS             8915..10264
                     /locus_tag="PLB46_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRF75759.1"
                     /translation="MKNPVSNFCKFVTSALLFVAFTQPVFSQAIPVTIQQDADGNWTL
                     YRNNEPYYVKGVGGSVELDKAVSIGANSIRTWGIDNAGEILDAAQEKGLTVLLGLWLQ
                     HERHGFDYDNAEKVKQQLAHFTEVVEKYKNHPALLAWGVGNEVDLFYTNTNVWYAVND
                     IAKMIHEKDPNHPTMTVTAGLDTTETRLLLERCPDIDIYGINTYGDLGNVAKQLRTTK
                     WNKPYMITEWGPNGHWEVQKTIWQVPVEQSSSEKAISYKERYAKYIAGDRKMCMGSYV
                     FLWGQKQETTATWYGLFDEKGLSSQAVDELERIWTGKEPINTAPVLTDVKLNGQQKGD
                     SVLIQPYSTCSAIATATDANGDKLKYVWEVVYESTDIKAGGDVEAKPAALKGLGIKTK
                     GNTVTFTAPEKEGPYRLYLFIYDGEGHYAYQNIPFYVLPGDAATDKGPALMWKKQSL"
BASE COUNT         3437 a         1913 c         1937 g         2973 t
ORIGIN      
        1 ataaccactg cattttgcac cgatacacaa tgtgatactg ttgtaattgc accaaccgga
       61 attgatgaag aaacaattat tggtgtatta aacgcatacc caaatccggc aaatgacctc
      121 atcaatatta cattggaaaa tgccattact gccaatacat caattgtgat taatgattta
      181 agtggtaaac aattaaaaga aattttcaca ggaacattgt acagtggtca acataatttc
      241 caaaccaata tcaatgtttt acctgaaggc atctatttaa tacatgtaat aagcgatgcc
      301 ggtggtgtgc atgtgattaa atttattaaa caataaatct cagcaaaagc gttcggttgt
      361 agttgtttga ttaaacgttg caggaaactc ctatcctgcc ttcaaaaccc cttcttggtc
      421 gaggggtttt gtcgttttat aagggtttgt aatcgccttg ttgagctgtg catgccaccg
      481 ggttgtttgt cttttcaggg tagattgaag gctattaagt aactttgtgc tacattataa
      541 gctatgacag acgttcaacc atatcaacca aagcataaaa taagaattgt aactgctgcc
      601 agcctgttcg atggccacga tgcagcgatt aatgttatgc gcaggataat ccaggctacc
      661 ggggtagaag taattcatct tggccatgac agaagtgtag aagaggtggt taattgtgcc
      721 atacaggaag acgttcaggc tattgcgatg accagttatc agggaggaca taccgaatat
      781 tttaaatata tgtatgacct gctcaaacaa aaaggctgtg agcatataaa aatatttggt
      841 ggtggcggtg gcactatttt gccgtctgaa attgaggagt tacaaaacta tggtatcacc
      901 agaatttatt ctcctgatga tggaagagca atgggcttac aaggtatgat taacgacctt
      961 gtccaaaaaa gtgattttgc taccggaatt aatttaaatg gagaactcac acatcttacc
     1021 aaagaaagag attggaaaag tatcggcaaa ttaatttctg ccgtagaaaa taacacacct
     1081 gatgcatcgg gtataatgac acaggtagaa gtattaataa aaaataataa tacacctgta
     1141 ttaggtatca caggaacagg tggagcagga aaatcatctt tagttgatga attagtgcgc
     1201 agatttttaa ttgattttac ggataaaaca attgccattt tatcggttga ccctagtaag
     1261 cgtaaaactg gtggtgcgtt gcttggcgac agaatcagaa tgaatgcaat aaataacgca
     1321 cgtgtttata tgcgttcaat ggcaacacgt caggcgaatt tagcattgag taaacacgta
     1381 gatgaagcag ttaaaatatt aaaagcagca cagttcgatt taattattct ggaaacttca
     1441 ggtattgggc aaagtgatac agaaattacc gaacacagca atgtgagttt atatgtgatg
     1501 acacctgaat atggcgctgc aagtcagctc gaaaaaattg acatgctcga ttttgccgat
     1561 ttaattgcca ttaataaatt cgataaacgc ggagctcagg atgcattacg tgatgtgcgc
     1621 aaacagtttc agcgcaatca taaattgttt gatgttgaaa cagaaaaaat gcctgtgttt
     1681 gcaactatcg catcgcagtt taacgacccg ggcaccaacc agttgtatcg tcatttaatg
     1741 gataccatta aagaaaaagc aaaggttgac ttacattccg attttcatat tacggatgag
     1801 atgagtaaaa aaatattcat cattcctcca cagcgtactc gttacctagc tgaaatttca
     1861 gaaaacaacc gcgcttacga taaaaaggta gttgagcaaa aaactgtagc gcaaaattta
     1921 tatgcactac atacttctaa agcaattgta agtgatgcag aattaaaaaa taaacttgaa
     1981 gaggaatacc aacgcgtagc taaaaaaata gataatgaaa atctggattt aattcattca
     2041 tggaataaaa aattagcatc gtataaagat gaatattaca tttttaaggt gcgcgataaa
     2101 gaattaaaaa taaaaacaca ttcagaaagt ttatcgcacc tggaaattcc aaaaatcagt
     2161 ctccctaaat atgaagcatg gggtgatatt ttacaatggc aattgcagga aaatgtgccg
     2221 ggtgaatttc catttaccgc aggtatttat ccatttaaac gcgaagaaga agacccaaca
     2281 cgtatgtttg ccggtgaagg cggacctgag cgcaccaaca aacgttttca ttatgtgagt
     2341 ttaggtatgc ctgcaaaacg attgtcaact gcattcgata gtgtaacatt atatggcaat
     2401 gaccctgatt atcgccctga tatttatggt aaaatcggta acagcggtgt gagcatttgt
     2461 tgtttagatg atgcgaaaaa attatatagc ggtttcgatt tagccgaccc taaaacatca
     2521 gtgtcaatga ccattaatgg tcctgctcct atgttgcttg ggttttttat gaatgcagct
     2581 attgatcagc agtgtgaaaa atatattatt gaaaataaat tagaagccgc ggttgaaact
     2641 aaaattcagg aaatatataa aggacgtgaa cacctcagac ctaaatacaa tgccgattcg
     2701 ttgcctacgg gtaataatgg ccttggatta ctattattag gcgttactgg cgaccaggtt
     2761 ttacctgcgg atgtgtatgc agtaattaaa gctaaaacac tgagcactgt tcgcggtact
     2821 gtgcaagccg atattttaaa agaagaccag gcgcaaaata cttgtatatt ttctactgaa
     2881 tttgcattgc gtttaatggg cgatgtgcag gaatatttta ttcagcaaaa agtccgcaat
     2941 ttttattcgg tttcaatttc aggttatcat attgctgaag cgggtgcaaa tccaattacg
     3001 caattagcat ttacattggc taacggattt acttatgtag aatattattt aagccgtggc
     3061 atgaacatta acgattttgg tcctaacctt tcgtttttct tcagcaatgg aattgatccg
     3121 gaatacgctg taattggtcg cgtagcacgc cgtatttggg caaaggctat gaaattaaaa
     3181 tatggcgccg acgacagaac acaaaaatta aaatatcata ttcaaaccag cggaagaagc
     3241 ttacacgcgc aggaaatcga tttcaacgat attagaacaa cgctgcaggc attatatgcg
     3301 atttatgata attgtaattc attgcatacc aatgcatatg atgaagccat tacaacacca
     3361 actgaagaaa gtgtgcgcag ggcaatggct atccaattaa ttatcaataa agaattaggg
     3421 ttagcaaaaa atgaaaatcc tattcaaggt tcatttataa ttgaagaact tactaatcta
     3481 gttgaggaag ccgtattaag tgaattcgac cgaattactg aacgtggcgg tgttttaggt
     3541 gcaatggaaa cgatgtatca gcgcggaaaa attcaggaag aaagtttatt ttatgaaaca
     3601 cttaaacata ccggcgaata tcccataatt ggtgtaaata cgtttttaaa ttcaaagggt
     3661 tcgccaacta ttcttcctat ggaagttatt cgtgcaactg aagaagaaaa agaagcacaa
     3721 ataactactt taaaaaatct gcatgaaaaa aatgcagaat taactgctaa acatttacgt
     3781 catttacaaa ctacggccat acagaacaac aatttatttg aagcattaat ggaagcttgt
     3841 aaattctgct cactcggaca aattaccaaa tcgctgtttg aggttggtgg gcaatataga
     3901 cgaaacatgt gattgccggg aagacgggaa ggcgggaagt ttcgggatga tggttgatga
     3961 ggtgcgtgat tcattgcctt aatcagtatg ggattaaaat ttttattatt taaggaaaga
     4021 actactcata aaaaaccccg gctatgttaa acacaaccgg ggttgaaact aaccgtacta
     4081 aattatttat tgaacagata aactgtttgc ataaagtatt tgtccgtttt taattacgac
     4141 tacctgatat acgccaggta ccatattatt cagattaaat gcatgattat ttccaacaat
     4201 ggtagattgt tgaatcattc ttccggtaat atcggtaatg attgcatttg cattaataaa
     4261 tatttgatct tcaaatgtta aataagcaac accttttgca ggttgtggcc aaacactaac
     4321 atctccaata acaatatcat taatactcac atcacaagct tcacattttg accaacaaac
     4381 tgcatctaaa gtagctggac ctgtaacggt aagtgcacga ttggtaaatc catctgtagt
     4441 gatggtacaa tcgtcaccgg aagttaattc ttcctgacct gcccaagcat cgtatgcaaa
     4501 tttgtattca taagttcctt ccggtaataa aatgtctaat tcccaaatat tatcaccatc
     4561 taaatcgctc aatggcgcac aacctccgca ccatccgttg aacgtaccat ttacttcagg
     4621 tgtagtaaat gctgcagcaa ctgtactcat gtctacctgg aatttaacaa tatgtgcagg
     4681 aactgctgtt tcacaagatg tacaacttcc ccaacaaaca gtatctaaag caaccggtgc
     4741 agttaccact aatgaacggt tggtaaatcc aaacgctgta atagtacaag gatcaccttc
     4801 taataatgtt tcggaacctg cccaagtatc gtaagcaaat ttatattcgt aagtacctgc
     4861 ttctaaagga atagttaatt cccaaatatt atcaccgtct aaatcgctca ttggtgcaca
     4921 acctccgcac catccgttaa atgtaccatt tacttcaggt gtagtaaatg cagcggcaac
     4981 ttcattcata tcaacagtaa ataaaacatc ataagtaact gctgcaccac aatccaaaca
     5041 acttccccaa catacagggt ccattaccat atcgctgctt acttctaaaa cgcggttaac
     5101 aaatccacca tcattaatag tacaaactgc accatcagct aatatttctg aaccagccca
     5161 agcatcataa gcatatttaa attgataagt accttcaggg attgataatg ttgtactcca
     5221 aataccatca ccatcagggt cagataatgg attacatcca ccacaccatc cgttaaatgt
     5281 tccattcact tcaggtgtag taaatcctgc aactacttca ttcatattta cattaaatgt
     5341 aacattgtaa tttaccacta ctaccggttc accaaacatt acatcatcaa aataataagt
     5401 tttttcacct gcaactgctc cggttacgcc gaagttgaag aagatagaag ctttatcata
     5461 attatatcca taattaattt cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnncaaggtg atcttctact tttaatctaa cctgaatgcc tgcatccggt gaccaaacac
     5641 gaacagtcat ggttgttgca tcaacggtaa aaggaatatc tgtagcaaaa cctgaacctg
     5701 caccatcagt tactgttgtt ccagcccata attcagctgc acccgattta attactttag
     5761 caaccatatt gcctgcatca gtagggtcaa cttcaattgt agaagcttct gctccaccga
     5821 atcctattaa accataatct acacctgctt catcgaatgt taccggtaaa tccatttgag
     5881 ctaaaaccac tccgtcgcaa tcatcacaac ttccccaaca aacctgatta agcaccatgt
     5941 ctgcttcacc caaagtgaat gttctatttg taaaaccaaa tgcagtaact gtgcaaggtg
     6001 aaccttctgt taaagattcc tgtcctgccc aagcatcata ggcaaatttg tattcgtaag
     6061 tacctggagc taaagcaatt gtaacttccc agatgccatt tgcatcgtcg tcgaataatg
     6121 gattacaacc accgcaccaa ccattaaaag taccattaac ctctggtgtg gtatacgctg
     6181 ctgccacctc gttcatgttt accttaaagg taacattgta ggtttgcgcc atggcactgg
     6241 tagccagggt gcaaagcagc aaataagtaa atatttgttt catagtaaaa ttattttaca
     6301 gtaaaaatag tcctttttgt aattattaca aaaagcaaaa tcaattaata ttttttttgg
     6361 gggaattgtt taatcacaac ttgcacaaaa tggttaaaac aggggaattt taacatttgg
     6421 atggaagttg taattcgccg caactacagc ttaatttttg catatccaat cgaatcttga
     6481 gttgaacaaa tgattttctg tataattaaa tattctattt aatttttatc taatccaatc
     6541 gaattgtttt tataacaaag acgcattttt ataaaaaaaa caggactacc cttttggagt
     6601 agtcccaccc ttaaacttaa aattcaaaat ttattcaaca attaaagttt tggtaactga
     6661 tgtattaccg gtattaattt taaccatata catacctgaa gccaaagtat ttcctaaaga
     6721 aattgtttgg ttaggatttc cattgtcaat agtatttgta gtttgataaa caactgcacc
     6781 tgttgaagaa taaatagcaa tatttattgc atcagcagta agtgaagcgg tttgaacttg
     6841 aatagtgaaa tcaccattgt ttggatttgg atagatagca aatacatcat catttgcacc
     6901 ttccaattta catgatgtag ttaccacaac agtagccgat tttgtgcaac ctgcaggtgt
     6961 tgtttctttt aatttataac taccaattga agtagcaacc aaatttaatg gagcaccaac
     7021 tattgcaaca ccatctttat accattgata tgtgctacca acaccaattt taccttttaa
     7081 taatactgaa cctgtagcac aaatatcaag actacctaca ggctgaattt taggttttgg
     7141 ttttgaaaca actaaagtag tagctgcaga ttgtgcaaat gcagttcctt gagaaataac
     7201 tacactataa ttacctgcct gattggctgt ataatttgaa gaagttgcac caggaatatt
     7261 tacaccattt ttcttccatt gatatccggt atatcctgtt ggtgcagcaa aagcctgagt
     7321 tgttcctgag caaattgtaa ctgtcccggc aggagcaatt gtagtattaa tgaaatcaca
     7381 agttccccaa attacagggt caagtgttgc gttatcagta accactaaag atctatttgt
     7441 aaatccgaag ttagtaacgg tacaaggaga accttccgtt aaagcttcct gacctgtcca
     7501 tgcatcataa gcaaatttat attcatatgt gcccggagcc aaagtaatcg tgatagccca
     7561 aatgccatcg ccatcaacat cgctcatagg agcacaacca ccgcaccatc cattaaaatt
     7621 gccattcact tccggagtgg taaatggtcc tgcaactcca ttcatgttaa cctgaaaagt
     7681 aatatcgtat aaagcgcctt ctccacaatt atcacaactt gcccaacata caggatccat
     7741 tacaatgtca gctgcatcaa ctactaaaga gcgattggtg aagccaaaat tagtaaccgt
     7801 acaaaatgtt cccggtacaa ggttttcctg accggtccag ctgtcataag caaatttgta
     7861 ctcataagta cctggtgcaa gtgcaatggt aacttcccaa attccgtcta aatcagggtc
     7921 actcattggc gcacaacctc cgcaccaacc gttaaaactg ccatttacct caggtgtggt
     7981 aaaaggttca gcaacttcat tcatgttcac cttgaaggtg acattgtagg tttgagccgt
     8041 ggcaaacagg ctgaaaccca atagcacaaa gaaaagcgta agatttttca tagaaaaaca
     8101 tttaggttaa agtgaaataa tgggataaat gtaattactt tttgtaattt ttacaataag
     8161 tattattttt ataatcttgt ttacatttgc cggatagaga tttgtatggc taaaacaaac
     8221 aaaaccctca gaaggcgaaa tgaataccaa atttatgatg tgcctattga ttcttttttc
     8281 gaattggcat tcagcataga ctgtgtggta ttcggctatg gaaagaagga cttacaggta
     8341 ttactaattc agcgcggagc cgagccctac aagggtttgt gggctttacc gggcgacttg
     8401 gtttaccctg atgaagacct cgatacctcc gccggaagag tcttaaaagc gcttaccagt
     8461 ctgcctgatt tgaactttga acaggtgcat acgtttggtg cggtagacag gcatccgttg
     8521 ggtagggtgg ttactgtggc ctattatgct atagtaaata tgaagggttt taacccaaaa
     8581 gcagcttttt gggcagacaa agcgaagtgg catagtgtaa aaaatttgcc aaagttagct
     8641 ttcgaccaca gggatattat tgattattcc tacaaaaagc taaaagaaag taccatgact
     8701 aaaccattgt ggaatggtgt gattgaagaa aaattcacgc tcactcaact acaatcgttt
     8761 tttgaaacgg tgctcgacaa aaaatttgat aaaggtaatt tcagaaaaaa aattgacctt
     8821 ttccaatatg taaaagaaac cgaagaattt gaaaaagatg tgccttatcg cccatcacga
     8881 ttattcaaat tcgacaaaaa actctatcaa aaaaatgaag aatccagtta gcaacttttg
     8941 caaatttgta acatcagccc tattgtttgt ggcatttaca caacctgttt tttcacaagc
     9001 aataccggtc acgatacaac aagatgcaga tggcaactgg acattatatc gcaacaacga
     9061 accttattat gttaaaggtg ttggcggcag cgttgaactc gacaaggctg ttagcattgg
     9121 tgcaaattct ataagaacat ggggaattga caatgcagga gaaattcttg acgctgcgca
     9181 agaaaaaggt ttaactgtat tattaggatt atggttacaa catgaacgcc atggttttga
     9241 ttatgataat gcagaaaaag taaaacagca attagcacat tttactgaag tagtagaaaa
     9301 atataaaaat caccctgcat tactcgcttg gggtgtaggt aacgaagttg atttatttta
     9361 taccaatacc aatgtttggt acgcagtaaa tgatattgca aaaatgattc atgaaaaaga
     9421 tccgaatcat ccaactatga cagttactgc aggattagat acaactgaaa ccagattact
     9481 tttggaacgt tgtcctgaca ttgatattta tggcattaat acttatggcg atttaggtaa
     9541 tgtagctaaa caattacgca ctactaaatg gaataaacca tacatgatta ccgaatgggg
     9601 accaaacggt cactgggaag tgcaaaaaac aatttggcag gttcctgtag aacaaagcag
     9661 cagtgaaaaa gcaatttctt ataaagaacg ttatgctaaa tatattgccg gcgatagaaa
     9721 aatgtgtatg ggttcatacg tttttttatg gggacaaaaa caagaaacaa ctgcaacatg
     9781 gtacggcttg tttgatgaaa aaggattatc tagtcaggca gttgatgaac tggaaagaat
     9841 atggaccgga aaagaaccaa ttaatactgc tccggtttta actgatgtaa aattaaatgg
     9901 ccaacaaaaa ggtgattccg ttttaataca accttatagc acctgttctg caattgcaac
     9961 tgcaactgat gcaaatggcg ataaattaaa atatgtttgg gaagtagttt acgaaagtac
    10021 cgatattaaa gccggtggtg atgtagaagc aaaacctgct gcattaaaag gtttaggaat
    10081 taaaacaaaa ggtaatactg ttacatttac tgctcccgaa aaagaaggtc cttatcgcct
    10141 ctatttattt atctacgatg gcgaaggaca ttatgcttac caaaatattc cgttttatgt
    10201 tttaccgggt gatgctgcta cagataaagg gccggcgttg atgtggaaga agcagagtct
    10261 ttaatgtgct gatttgttaa tgtgctgatg tgctgattaa tgtgagaatg taccaatgta
    10321 ccgatgtacc aattcgaacc gtccgctgag gcggaaacag
//
LOCUS       DAORKW010000063        10317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_137_length_10317_cov_6.373124, whole genome shotgun sequence.
ACCESSION   DAORKW010000063 DAORKW010000000
VERSION     DAORKW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10317
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137_length_10317_cov_6.373124"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..81)
                     /locus_tag="PLB46_04260"
     CDS             complement(<1..81)
                     /locus_tag="PLB46_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRF75760.1"
                     /translation="MQVEIWSDIMCPFCYIGKRKFEIALSQ"
     gene            226..2016
                     /locus_tag="PLB46_04265"
     CDS             226..2016
                     /locus_tag="PLB46_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75761.1"
                     /translation="MTLYRFLLLVLTTVIIGTDISAQTATYTPAEATLDSAVHNYNAM
                     RDFAGSLTSASITDADLQRIATQREIINRQLERVIQTGTADNISVAKYFKLFSDYEYG
                     FMLGVANKLNESYRILDPLKSQMKDLEPSDFSKYYLFENKTYTIKWENAAPTILEYYS
                     SMTELYNNQKNYTAAIEMAQSGFNLAYSPENNDNKNFLKYICITQYLVAANQLFKYNY
                     EDAMMAINQITYFQLLDEDNKKRITENGSFGYASGYNFINTIKTYNPGLTSEDEIYAK
                     AATALKNGNDFERGPVMYIKALNAGYYNYTFIQEAFSMADLAKDNKLGLLATEKYEQQ
                     ISSSDCAAWREIAIKWNTYGNSQKSNAANEKAKSCEQIAAEAQKAYEKEQARRSRIAD
                     RDFSMYAGIYPLPMIIRFNKYRDYGGVAGFGIRKFSIEVSYKKINLNHVIYDDLFIQE
                     IDVNDFDNYWSGYRAHVAFKFGERDTYSDGFFVGPLFEIVQRNYEPVTSAVFANDAFS
                     YLYDADFYPKETSYNAMLNFGTRIEENHIMFEYFTGIGVAYHQFDGGGIEYGNADFYL
                     SNPVLQNRKPERFGPVVRMGVTFGLSTRGN"
     gene            complement(2040..2615)
                     /locus_tag="PLB46_04270"
     CDS             complement(2040..2615)
                     /locus_tag="PLB46_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HRF75762.1"
                     /translation="MRYSLILILSILLGGCGLNKPLSVVQPLSVEQPTSLETTNEISD
                     TLIIINRAPSGMQAMDEVTVLPSDRTTKIIQQQNFRKKSITTLQQEQPNQLKKKKLNA
                     DDEPTPTKPKMNIWAIMGFVFVLTGVIPIIGNLVGYIMCIVALNQINKNPGMYTGKGL
                     ALAGFVIFTIFLVAAITIALFYFGLLSFNGS"
     gene            complement(2733..3272)
                     /locus_tag="PLB46_04275"
     CDS             complement(2733..3272)
                     /locus_tag="PLB46_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75763.1"
                     /translation="MRFSHLFALFLAITVNKAKCQDFINYDKEIVVDFLFDGGYDTSR
                     GSEYLLLMDSLKITAMFNENYCSILEYYFYKDEDDYLICDSIYLSSNCERCFEVNLRE
                     LLSKKSRRWKKINDSLYISSNNVGSITHYGPPKETTYWVLKLEIQHSNDNTNSSLYIH
                     AILIDEADYKKNKKLPRYK"
     gene            3494..3856
                     /locus_tag="PLB46_04280"
     CDS             3494..3856
                     /locus_tag="PLB46_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRF75764.1"
                     /translation="MPYVCTMNVENAQAQMRKGVLEYCILAIINEGEIYPTDVINRLK
                     ESNLLVAEGTVYPLLNRLKTMELLSYKWVESNSGPPRKYYQLTDNGRLFLTELNKTWQ
                     ELAHAVSINTKSLNTTQQ"
     gene            3853..5418
                     /locus_tag="PLB46_04285"
     CDS             3853..5418
                     /locus_tag="PLB46_04285"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRF75765.1"
                     /translation="MNKLLYVNIGGVVFQIDETAYQQLDNYLNSIRRKYASTPDGEEI
                     IKDIEHRIAELMYERVGERGAITHAHVEDIIGIMGKPEAYDSQPEAEEKTTYNEYTQR
                     PGATKFFRDKENNVLGGVCAGFGAKFDIDPLWIRLAFLAFFFVAGTGFLLYIILWAII
                     PEAKTTAEKLEMRGEKVDINNIEKTVKDGARQFSKKVNEFGEEVKQTFSKENIDKSKR
                     NAGDFIEDAAQTLGPVVKTIVKVFVFGILIVSLMVVVVTGVELFTNWGTDFREIEFFG
                     NHITEGSQQAWILVGCAVSLVIIPFLGIIFSSVKYLLGIKRKTKFVATTLGFIWIACL
                     LVVIYLGISIGRNFKYEASVSNKMTISQPVNQAMHIQLDAIPENNMEHGIVSWSIKKD
                     DFDDSLMFKHISVEFEKSLDTNFAVLLNKTARGYDRNNAKNNAQELNFPVTQNADSII
                     TIPSLIQLAEKEKWRGQKVDIIIRVPENKYVYIDNRLDYYLDKNEYTSGLRDVELFNN
                     KLLMTSTGLKPVN"
     gene            5478..6002
                     /locus_tag="PLB46_04290"
     CDS             5478..6002
                     /locus_tag="PLB46_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75766.1"
                     /translation="MQVSPAHWHLLINHFPIILSIVGTLFIGLSFMLKKNTLINGGLI
                     LLVMAALTALPANATGEGAEESVEGITGVTHDGIEIHEHAASTGLKILIGTGILALIT
                     AFAQVKMPKRAMVLLFVTFIAGVASAGYMSYVGLTGGEIRHSEIRGDFGRSASAATPG
                     QTTGNQFEAEEDDD"
     gene            6111..7142
                     /locus_tag="PLB46_04295"
     CDS             6111..7142
                     /locus_tag="PLB46_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75767.1"
                     /translation="MFKWLSYLSIFSILNLSCSKNNNSVTRGFYFWQSSLNSKTLNYN
                     RLDSLNISSLYVKFFDVTWDETSQQPIPVADISLEQPIPANFSVIPVVFITNKTLEHL
                     DTIQTKYLAQKIAKRIQYIIDTNNLAQPTEIQIDCDWTATTRQNYFSLLQTIKQYPAF
                     NKIQISATIRLHQIKYYKTTGVPPVDKGLLMFYNMGNIEDDKSVNSIYDENIATQYVD
                     YIKAYPLALDAAIACYSWGLLYDNHKLLRIFYPLYQDEISDSLFLKVGDNTYKANGNF
                     YFEGQFFVSGNILKLETMTPELSLRAAEQLAENLHNEKINVILFHLDEIILKKYSNED
                     LEAIYNSFE"
     gene            7105..9426
                     /locus_tag="PLB46_04300"
     CDS             7105..9426
                     /locus_tag="PLB46_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75768.1"
                     /translation="MKIWKPFTIVLSSLLITFPLHLANTCSWGYDMDESILSPFHSEI
                     LDLPELFPFYYSEHFYNGDPNSDWSENGGSMDEDLFDGTDNNINEWFGYFNNAVTKED
                     ITAIIYQTQVSDYEAFANHIKGKKNAVEAKWLTNSVLKFWENNPKDPSFRYLTLAKQI
                     EPLVQPVYWWDEIRTDTMRLVDYKNDALGQLKKSKSEFITLRYAYQAARAAHYTGNYQ
                     ECISIFQKYVAPVQSESQIKYWTMSLMAGAEQRLKNYAVAAYYHAMVFNKCWSRRLSS
                     KRDFYIDNDTIWQQCLNLCKNDHEKNTLWFLTGVSQYNSAVPALNEMLKIDPSSKEIE
                     LLLSREIEKIQRNNMPERWWSGIDYEGDFEYQRTSIAPNDIAEIIDVLEKGIAINKMH
                     TPVFWYNAAAFLYFIVEDAEKCKSMCALAIQHANGDNNQIQQAKIISILNKVNNAGEI
                     KSSVEKELIQDLKWLAEQDNTNEMSGFKADAYRIVMFQLMEYYLNDDKPLLAEMCRAR
                     AVEYYDIYLEPEKAPIEELYTFLTSNKKSDFESFLAGQYPYNADHMLEIKGTLLLREN
                     QLEEAIRTFKKIQSPDGILYPLSADPFVIHINDCHDCDFEAYPTDLNKLDLAEKMLAL
                     KSLAKSDALNRAQIYHELGNAYYNISYWGNAWNALDYYRCHGCETYNIGENASDYYYY
                     NDEYDLTNAVFYYTLAVQETSGKQFAALNYFMLAKCEQNNFYRSAEITDYKFKTDDQQ
                     IYFGILHELFSDTEFYREAINECKYFNYYVSNH"
     gene            9530..>10317
                     /locus_tag="PLB46_04305"
     CDS             9530..>10317
                     /locus_tag="PLB46_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75769.1"
                     /translation="MKQLVSAALLLTLTLLNNTLFSQPLEWQSRGIGGGGALFAPSIN
                     PVNDAEMYIGCDMTEVFHTTNSGLSWNTVGFNELLSVTTSKVQFTSDNLIRYAVNLDF
                     FTEASFPVKSIDGGNTWSPITDPSSGECYAIFADDNSTQRIVIASYNRIYFSSNGGTT
                     FTNIYTNMGSGAAHVGGVFFDGTNIYIGSAKNLIVSTDNGASFTSYTLTGLPAGRGIM
                     SFAGAKSGATTRFFIVSYDLVDIYPGVTGADYFSYKSIYKMDYA"
BASE COUNT         3470 a         1757 c         1895 g         3195 t
ORIGIN      
        1 ttgggaaaga gcgatttcaa atttccgttt tcctatataa caaaacggac acatgatatc
       61 cgaccaaatt tcaacttgca tgatattacg ctttaatcaa taaaccgcaa tattaacaga
      121 tttagttcat tataaatgaa gctgcatttg gttatgatga aactattgga gccagcgggt
      181 ggcgtttaat aacgatttaa tacatttgta tcgaaattaa aaacaatgac attatacaga
      241 tttttactgc ttgttttaac tacagttatt ataggaacag acatatcagc acaaacagca
      301 acatatacgc cggcagaagc aactttggac tcggcagttc acaattataa tgctatgcgc
      361 gattttgccg gttcactaac ttctgcaagt attaccgatg ccgatttaca aagaatagct
      421 acccaacgtg aaatcattaa cagacaattg gagagagtta ttcaaacagg cacagcagat
      481 aatataagtg ttgctaagta ttttaagctg tttagcgatt atgaatatgg atttatgtta
      541 ggtgttgcaa ataaattaaa cgaatcgtat cgcatattag accccttgaa atctcaaatg
      601 aaagacctgg aaccttcaga ttttagtaaa tattatttat ttgaaaataa aacctatacc
      661 atcaaatggg aaaatgccgc gccaacaatt ttagagtatt attcttcaat gacggaacta
      721 tacaacaatc aaaaaaacta tactgcagca attgaaatgg ctcagagcgg atttaattta
      781 gcatattcac ctgaaaataa tgataacaaa aattttctta aatatatctg tataacacaa
      841 tatttagtcg ctgcaaatca attgtttaaa tataattatg aagatgcgat gatggcaatt
      901 aatcaaatta cctattttca gctacttgat gaagataaca aaaaacgaat aactgaaaat
      961 ggcagctttg gttatgcatc cggatataat ttcatcaata caattaaaac gtataacccc
     1021 ggcttaactt cagaagatga aatttatgct aaagcagcta ctgcattaaa aaatggaaat
     1081 gattttgaac gcggtcccgt aatgtatatt aaggcattga atgccggtta ttataattat
     1141 acatttatcc aggaggcatt tagtatggcc gatttagcaa aagataataa attgggtttg
     1201 cttgccactg aaaagtacga acaacaaata agttcaagcg actgtgcggc atggcgtgaa
     1261 atagccatta aatggaatac atatggcaat agccaaaaaa gcaatgcagc aaatgaaaag
     1321 gcaaaatctt gcgaacaaat tgcagctgaa gctcaaaaag cctatgaaaa agaacaagca
     1381 agaagaagcc gaattgcaga tcgtgatttt agtatgtatg caggaatata tcccttgcca
     1441 atgattattc gttttaataa atatcgcgac tatggtggag ttgcaggatt tgggattcgt
     1501 aaattttcga ttgaagtgag ttataaaaaa attaatttaa accatgttat ttacgatgat
     1561 ttgtttattc aggaaattga tgtaaacgat tttgataact attggagtgg ttacagggcg
     1621 cacgttgcct tcaaatttgg cgaacgcgat acttatagcg atggattttt tgttgggccc
     1681 ctattcgaaa tagtacaaag aaattacgaa ccagttacta gtgccgtttt tgccaatgat
     1741 gcatttagtt atttatacga tgccgatttt tatcccaaag aaacaagtta taatgcaatg
     1801 ctcaattttg gcaccaggat tgaagaaaat catattatgt ttgaatattt caccgggata
     1861 ggtgttgctt atcatcaatt tgatggtggg ggtatagaat atgggaatgc tgatttttat
     1921 ttaagtaacc ctgtattgca aaaccgcaaa cctgaacgtt ttgggcctgt tgtcagaatg
     1981 ggtgtaacct ttggactttc taccaggggt aattaatggt gaaataacca ccaattaatc
     2041 taggagccat taaatgataa taacccaaaa taaaacaagg caattgtaat agccgcaact
     2101 aaaaatatag tgaaaataac aaatcctgct aaggcgagcc ctttacctgt atacatgccg
     2161 ggatttttat tgatttgatt tagggctact atacacataa tgtaaccaac taaattgcca
     2221 attatcggaa taactccggt taacacaaaa acaaatccca taattgccca gatattcatt
     2281 ttaggttttg tcggtgttgg ctcgtcatct gcatttaatt ttttcttttt aagttggttc
     2341 ggttgctctt gctgtaatgt tgtaattgat ttcttccgga aattttgttg ttgaataatt
     2401 tttgttgttc tgtccgatgg caatactgtt acttcatcca tggcttgcat tccggatgga
     2461 gccctgttga ttattattaa tgtatctgat atttcattgg ttgtctccag tgaagtaggt
     2521 tgttcaacgg acaatggctg caccacagac aggggcttat ttagtccgca tccacctaat
     2581 agtatactta aaatgaggat tagtgagtat cgcatgacaa aatgttacat aaattgttaa
     2641 acattaatgc taatggtggt tcttttaatt acagtaggca ttatttaagt tgaactcaat
     2701 aatacgaatt atttttactg atatctaatt aattatttat accgtggtag ttttttgttt
     2761 tttttataat ctgcttcatc aattaatatt gcatgtatat ataaactcga attcgtatta
     2821 tcatttgaat gttgaatttc caattttagc acccaatacg ttgtttcctt cggaggtccg
     2881 taatgagtta tactgccaac attatttgag gaaatataca gactgtcgtt tatttttttc
     2941 caacgacgac ttttttttga taacaattca cgaagattaa cttcaaaaca tctctcacaa
     3001 ttgcttgaca aataaatact atcacaaatt aaataatcat cttcatcctt atagaaataa
     3061 tattcaagta tggaacaata gttttcattg aacatggcgg taatttttaa actatccatt
     3121 aggagcaaat attcactgcc cctgctcgtg tcatatcccc catcaaataa aaagtcgaca
     3181 actatttcct tgtcataatt aataaagtcc tggcattttg ccttattgac agtaattgct
     3241 aagaaaaggg caaaaagatg gctaaaacgc atcttataaa agtaattaac ctgactcttt
     3301 aaggcggggt tttgttgaaa aaaaaatttt tagcccaaaa gcgcaaattt tagggtgcac
     3361 tgtaaaaagg cctacaaatc aataccctgt tatgttatag ccgaatttta tcacacaatt
     3421 ttttctcttt aacaaaaatt taacacctag aactagtata aacatgatac tttgtattgc
     3481 atagttcagg tttatgccct atgtttgcac catgaatgta gaaaatgctc aggctcaaat
     3541 gcgaaaaggt gtgctggagt attgtatcct ggccatcata aatgaagggg agatctaccc
     3601 tactgatgtg ataaacaggc tcaaggaaag taatttgtta gtagcagagg gaactgttta
     3661 ccctttgttg aacagattaa aaacaatgga actcttaagc tacaaatggg tggaaagtaa
     3721 ttcaggtccg cctcgcaaat attatcagtt aactgataat ggccgcttgt ttttaactga
     3781 attaaataaa acatggcagg aattagctca tgccgtatca attaacacca aatcattaaa
     3841 cacaacgcaa caatgaacaa gttattatat gtaaatatag gtggagttgt ttttcagata
     3901 gacgaaactg cctaccaaca attggataac tatttaaaca gtatccgccg caaatatgct
     3961 tcaactcctg atggagaaga aataattaaa gatatagaac accgtatagc cgaacttatg
     4021 tatgaaagag ttggcgaacg cggagctatt actcatgctc atgtggaaga tattattgga
     4081 ataatgggta aaccggaagc ctatgattca caacccgaag cagaggaaaa aacgacgtat
     4141 aacgaataca ctcaacgtcc aggagcaact aagtttttcc gtgataaaga aaacaatgtg
     4201 ctcggcggtg tttgcgcagg atttggtgca aagttcgaca tcgacccact ttggataagg
     4261 ttggcgtttt tagctttctt ttttgtagcc ggaactggat tcctattata tatcatattg
     4321 tgggcaatta ttcccgaagc taaaaccaca gcagaaaaat tagaaatgcg tggtgagaaa
     4381 gtggacatca acaatattga aaaaacggta aaagacggtg ccaggcaatt cagtaaaaaa
     4441 gtaaatgaat ttggcgaaga agtaaaacaa accttttcga aagaaaatat cgataaatcg
     4501 aagcgcaacg ccggcgattt tattgaagac gccgcacaaa ctttgggtcc agttgtgaaa
     4561 actatcgtga aagtttttgt tttcggaatt ttaattgttt ccttaatggt agtggttgta
     4621 acaggtgttg aattgtttac caattggggt actgatttcc gtgagataga atttttcggc
     4681 aaccatatta ccgaaggttc acaacaagcc tggatattag ttggatgtgc ggtttcccta
     4741 gttattattc cttttttagg tatcattttt tccagtgtta aatatttatt aggtattaaa
     4801 cgcaaaacaa aatttgttgc caccactttg ggatttatct ggattgcttg tttgttggta
     4861 gtaatttatc tcggtatcag cattggtcgc aattttaaat atgaagccag tgtaagtaat
     4921 aaaatgacta tttcgcaacc ggtaaatcaa gctatgcata tacaattaga tgccattccg
     4981 gaaaataata tggaacatgg aatagttagt tggagtatta aaaaagatga ttttgacgac
     5041 agcttaatgt ttaaacatat cagtgttgaa tttgaaaaaa gtctggatac caattttgcc
     5101 gttctgctca acaaaacagc acgtggttac gatcgtaata atgctaaaaa caatgcgcag
     5161 gaattaaatt ttcctgtaac acaaaatgca gattccataa ttaccattcc atctcttatt
     5221 caattggctg aaaaagaaaa atggcgcgga caaaaggtgg acatcataat ccgtgttcct
     5281 gaaaataaat atgtatatat cgataatcgt ttagattatt atctggataa aaacgaatat
     5341 acctcgggat taagagatgt agaattattc aataataaat tattgatgac atcaacaggg
     5401 ttaaaaccgg taaattaaat ttgacaggca aactcaaagt ttaaatttat tttttaactt
     5461 taagtaaaac aattattatg caagtaagtc ccgcccattg gcatctcctg attaaccact
     5521 ttccaattat tttaagtata gttggtactt tatttatcgg attgagtttt atgctcaaaa
     5581 aaaacaccct catcaacgga ggattgatat tgttggtgat ggcagcctta accgctttgc
     5641 ctgcgaatgc tacaggcgaa ggtgcagaag aatcggtgga aggtattacc ggtgttacac
     5701 acgatggtat cgaaattcac gaacatgccg cgtcaacagg attaaaaata ttgataggca
     5761 ccggaatttt agcgctaata acagcctttg cgcaggtaaa aatgccaaaa cgtgctatgg
     5821 tattgctttt tgtcacattt attgcaggcg tggccagtgc cggttatatg agttatgttg
     5881 gattaaccgg tggtgaaatt cgccatagtg aaattcgtgg cgactttgga aggagcgcat
     5941 ctgcagcaac acccggccaa acaaccggca atcaattcga agcagaagaa gacgatgatt
     6001 aaaatatttg cttagttcaa ttagcgttgt atttaatgca aaaatcaaaa tcccagctca
     6061 aaaggcaggg atttttttat ggatatcaaa ttgtttgcga actttgaaac atgtttaagt
     6121 ggttaagcta tttgagtatt ttcagtatat tgaacttgag ctgctcaaaa aataataatt
     6181 cagttacacg tggattttat ttttggcaaa gcagtttaaa tagcaaaaca ttaaattaca
     6241 acagactaga ttcattaaac atttcatcat tgtatgtcaa attttttgat gttacatggg
     6301 atgaaacaag ccagcagcca attccggttg ctgatatcag ccttgaacaa cctattcctg
     6361 caaatttttc agttataccg gtagtattta ttaccaataa aacactggaa catctggata
     6421 caattcaaac caaatattta gcccaaaaaa tcgccaaacg tattcaatat attattgaca
     6481 caaacaattt agcacagccc actgaaatac aaattgactg cgattggaca gctacaacca
     6541 gacaaaacta tttttccctt ctacaaacaa taaaacaata tccggcattc aataaaattc
     6601 aaattagtgc aacaattcga ttgcaccaaa taaaatatta taaaacaacc ggagtgccgc
     6661 ctgtagataa aggtttactc atgttttaca atatgggtaa tattgaagat gataagtcgg
     6721 taaattccat ttatgatgaa aatattgcta ctcaatatgt agactacatc aaagcttatc
     6781 cccttgcatt agatgctgct attgcctgct acagttgggg gctattatat gacaaccaca
     6841 aattattaag aatattttat ccgctttatc aggatgaaat tagcgatagt ttatttttga
     6901 aagtaggtga caacacttat aaagcaaatg gtaattttta ttttgaaggg cagttttttg
     6961 taagtggcaa catattaaaa ttagaaacta tgacaccgga gttaagtttg cgcgctgcag
     7021 aacaacttgc tgagaattta cataatgaaa aaattaatgt aattttattt caccttgatg
     7081 aaatcattct aaaaaaatat tcaaatgaag atttggaagc catttacaat agttttgagt
     7141 agtttactga taacattccc attacatctc gccaacacat gcagctgggg atacgatatg
     7201 gacgagtcaa ttttatctcc tttccattcc gaaatacttg atttacctga gctatttccc
     7261 ttttattata gtgaacactt ttataatggt gaccccaatt cagattggag tgaaaatggt
     7321 ggcagtatgg acgaagattt atttgatggc accgacaata acattaacga gtggtttggc
     7381 tattttaata atgccgtaac taaagaggat atcactgcta ttatttatca aacacaggtt
     7441 agcgattatg aggcatttgc caaccacata aaaggcaaaa aaaatgcagt agaagctaaa
     7501 tggttgacaa atagtgtatt aaaattctgg gaaaataatc ccaaagaccc ttcgttccgt
     7561 tatttgacac ttgctaaaca aattgaacca cttgttcaac cggtgtattg gtgggatgaa
     7621 attcgcaccg acaccatgcg attagttgac tataaaaacg atgcacttgg gcaattgaaa
     7681 aaatcgaaat ccgaatttat tacactccgt tatgcttatc aggctgcccg cgctgcacat
     7741 tataccggca attatcaaga atgtatcagc atctttcaaa aatatgtggc tccggtgcaa
     7801 agtgagtcac aaataaagta ttggacaatg agtttaatgg caggtgccga acagcgatta
     7861 aaaaattatg ctgttgctgc atattatcac gctatggttt ttaataaatg ctggagccgc
     7921 agattaagtt caaaacgcga tttttatatt gacaatgata ccatttggca gcaatgttta
     7981 aacttatgta aaaacgatca cgaaaaaaac actttatggt ttttaaccgg tgtaagccaa
     8041 tataattctg ctgttcctgc attaaacgaa atgcttaaaa ttgacccttc atctaaagaa
     8101 atagaattat tactctcccg cgaaatagaa aaaattcaac gtaataatat gccggaacgt
     8161 tggtggagcg gcattgatta tgaaggagat tttgaatatc aacgcacatc aattgcacct
     8221 aacgatatag ctgaaattat tgatgtactg gaaaaaggta ttgccatcaa taaaatgcac
     8281 acaccggtat tctggtataa cgctgcagca tttttatact ttattgttga agatgctgaa
     8341 aaatgtaaaa gcatgtgtgc acttgccatt caacatgcca atggtgataa caaccaaatt
     8401 caacaggcaa aaattattag tatcctcaat aaagtaaaca acgccggtga aataaaatct
     8461 tcagtagaaa aggaattaat tcaggattta aaatggcttg ccgaacagga taatactaat
     8521 gaaatgtccg gatttaaagc tgatgcttac agaattgtta tgtttcagtt gatggaatat
     8581 tatttgaatg atgacaaacc cttattggca gaaatgtgtc gtgctcgtgc cgttgaatat
     8641 tacgatattt atttggagcc cgaaaaagca cctattgaag aattgtatac atttttaacc
     8701 tccaataaaa aatctgattt tgaatcattt ttggcggggc aatatcccta caacgccgac
     8761 cacatgcttg aaattaaagg caccttgctg ttgcgcgaga accaactgga agaagcaatc
     8821 cgcactttta aaaaaataca atctccggat ggtattttat acccattgtc ggctgaccca
     8881 tttgtaattc atattaacga ttgtcacgac tgcgattttg aagcttatcc aaccgattta
     8941 aataaactcg atttagcgga aaaaatgctg gcattaaaat cgctggcaaa atccgatgcg
     9001 ttgaatagag ctcaaattta tcacgaactc ggcaacgcat attacaatat atcttattgg
     9061 ggaaatgcgt ggaatgcgct ggactattac cgctgccatg gttgtgaaac gtataatatt
     9121 ggtgaaaatg ccagtgatta ttattactac aacgacgagt atgatttaac caatgctgta
     9181 ttctattata cattagcagt gcaagaaaca agtgggaaac aatttgctgc attaaattat
     9241 tttatgctgg ccaaatgcga acaaaataat ttttatcgca gtgctgaaat tactgactat
     9301 aaatttaaaa ccgacgacca acaaatttat ttcggaatat tacatgaatt attttctgat
     9361 accgagtttt accgggaagc catcaatgag tgtaaatatt ttaattatta tgtgagcaat
     9421 cattaatctt gcttcccccg attgtggtat tgtgaaatgc ccaaattttg gctattttta
     9481 catagtccgg cacgcactta ttttttaagc tttcaaccta atcgaccgta tgaagcaact
     9541 tgtatccgct gccctattac tcacactaac attattaaac aacacccttt tcagtcaacc
     9601 cctggaatgg caaagtcgcg gtattggcgg aggtggtgca ttatttgcac cttccattaa
     9661 ccctgtaaac gatgctgaaa tgtatatcgg ctgtgatatg accgaagtgt ttcacactac
     9721 aaattccggc ctcagctgga atacagttgg ttttaatgaa ctgctttctg taaccacttc
     9781 aaaagtgcaa ttcacttccg ataatttaat acgatatgct gtaaatcttg atttttttac
     9841 tgaagcttcg tttccggtaa aatcaataga tggcggaaat acttggtcgc caataacaga
     9901 cccttcaagc ggagaatgtt atgccatttt tgctgacgat aattcaacac aacgcattgt
     9961 aattgcatcc tataaccgca tttatttttc ctccaatggc ggaacaactt ttactaatat
    10021 ttataccaat atgggaagtg gtgcagcaca cgttggaggc gtatttttcg atggcactaa
    10081 tatttatatc ggctctgcaa aaaatttaat tgtaagtacc gataatggcg cttcatttac
    10141 cagttacaca ttaacaggtt tgcctgcagg gagaggtatt atgtcgtttg caggtgcaaa
    10201 gtctggcgca acaactcgtt ttttcattgt gagttacgac ctggttgata tttatcccgg
    10261 tgttaccgga gctgattatt tcagctataa aagcatttat aaaatggatt atgcgat
//
LOCUS       DAORKW010000064        10205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_141_length_10205_cov_4.809951, whole genome shotgun sequence.
ACCESSION   DAORKW010000064 DAORKW010000000
VERSION     DAORKW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141_length_10205_cov_4.809951"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..2032)
                     /locus_tag="PLB46_04310"
     CDS             complement(68..2032)
                     /locus_tag="PLB46_04310"
                     /inference="COORDINATES: protein motif:HMM:NF025697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HRF75770.1"
                     /translation="MQLKKLLKGKQSATAKMKLFSMALAVGLSLQSEAQTIYALSGGG
                     LYSIDAGMPESANFYGSISGITPGQTIEGMDFRPLNGMLYALGYDRNTQMAQIYTVDI
                     ATAIATPVNIAPISLVLGPVGADRLDVNFDFNPTVDRIRVTSNRDYNYRLNPITGAIA
                     FTDINLNYAVADVNAGANPYIATGAYTNSYVGSTSTVLYNVDALLKVVAKQDPPNNGT
                     LNTIGNLGGAVSKSFSSADMDIFYDAVSQTNKTYLTVNSNLEADRLISVDLTTGVGTL
                     ITTNIGGGLYIDDIACFIDRSYPELMGNLMYALNTNNFLLSYDSNNPSVIRKAVPVTG
                     ITLNQAIVGMDFRPATGALYALGYNSATNESQLYTINVSTGAATVVNAVPTILMLGGN
                     NIGMDFNPVVDKIRVVGANNANYRLNTDGTLFFTDLNLNYGAADINFGADPMIGAVAY
                     TNSFAGTLTTSLYDYDQMLNVISNQNPPNDGALITIGASGIMQNLTDPTTDMDILYKD
                     GMNIAYLTANTGVATFDRLYALDLTTGATTDMGAIGYGLAVKNIAATTNPELKIAGGI
                     INAAGKLQVDIFPNPVHDLLTVKFHTKEASLAQIAVFDIFGNNTGVGLAQLVSANASV
                     TLDVSPLVPGAYVIKINAEGKTISKNFVKL"
     gene            2253..4349
                     /locus_tag="PLB46_04315"
     CDS             2253..4349
                     /locus_tag="PLB46_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75771.1"
                     /translation="MKISRVLILVVILVEGLMANAQASFDHKVVEVGNMGFYVTNALT
                     LGRPDVRNSPSGEPSMEYPLNSGIEHLFEAGLWIGAIVDGQYLVSTSAKDAASGYTTG
                     QTGFEFSAEIGNTIQQRSSLTASDFFNFDAVSHADMLIDASDKNVIVPGTTIAISEHT
                     QPLNADVHLESYAWNYSFADYFVLLNYSITNNSTNIWDSVWCGLWSDLVVRNVNVSTD
                     FGSAFFSQGGYGYLADYFTNYAFDVDGDPGFTNSYGAFQFLGIEWRDQFIHPNNNVNL
                     IAAGLPAANVNSNFWIFNSTATPPYNAPANDVERYDKLQDGLNYADPGIVDFLHDPST
                     TGGLTNLLSAGPVIAIEPGETIHLVFAAVCAKQIDNGGVNGEEKDTPEAQATLVDHLS
                     WSKRTYLGEDQNENGILDAGEDLDADDILDRYILPEPPATPKIKVIANSQSIEIYWDN
                     KAEFSVDPISKEVDFEGYRLYRTQPGDDFNLNLLGDANMIAQWDLPGNNLGYNNGLQL
                     VALTTPEIIDADTFYYKYTLDNVLNGWQYLIILTAFDRGDENLNIESLESSFIENAVS
                     VFPGTLATSDEQTAIGVYPNPYRLNAAWDGATSTTRKIIFYHLPAKCEITIFTLGGDI
                     VATIQHDGDNYNGSDIGWFDTYGGNENAREFSGGEHAWDILSESKQTITQGIYLFNVK
                     DLETGINKQGRFVVIE"
     gene            4432..5664
                     /gene="serA"
                     /locus_tag="PLB46_04320"
     CDS             4432..5664
                     /gene="serA"
                     /locus_tag="PLB46_04320"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRF75772.1"
                     /translation="MSALTSYPKEKIQILLLENIADRAVQGFVDSGYAQVKKLSGALE
                     EDALIDAIKDVHLLGIRSKTLITSRVLQQANKLLAVGCYCIGTNQVDLQTAKEKGVVV
                     FNSPYPNTRSVAELVIAEAIMLLRRIPEKNTAAHQGIWLKDARNCFELRGKTLGIIGY
                     GNIGMQVSVLAEALGMRVIYTDVTTKLPMGNAQQIHQLYDLLKIADVVSLHVPEAPST
                     IQLLNDAALSQMKKGSILINCARGNVIDLKALKTHLLSGHIGGAAIDVFPEEPEKNGA
                     EFVSDLQQIPNTILTPHIGGSTEEAQVNISEDVTAKLIGFLESGVTSGSHTVPALHLP
                     QHPNAHRILHIHRNTPGVLSAINSVFSNNEVNIVGQYLNTNTEIGYAVIDVNRNLTTS
                     TIEALKNVPHTIKMRVVY"
     gene            5760..6545
                     /locus_tag="PLB46_04325"
     CDS             5760..6545
                     /locus_tag="PLB46_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HRF75773.1"
                     /translation="MKPIYSTTLALSLFITFNLNAAISNFCFVDEFGYIAEVTATRTG
                     PGYYELNGTADVFAGYDWIVTGYYDKTSDVWSLTYTNPFPDGCLVFTDYFVYTSTSHT
                     AGNINFDWTNYCFGGATDVGSASTIYFKSLCVFRLPESTPAGPASADKSLSGIKQFIA
                     TPQEGTPLFRDLLFDQEFSVTTTAENNYTISFELDNSTDVKIDIYNYAGQFVTTVTEA
                     AYNSGFYKLNWNGNDAAGNESKPGMYLAILHSDNEQLSYKFIK"
     gene            complement(6621..9305)
                     /locus_tag="PLB46_04330"
     CDS             complement(6621..9305)
                     /locus_tag="PLB46_04330"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HRF75774.1"
                     /translation="MPRKFLLLAISIFVNCMFLKASDGIFVYKNEQSNQNVVNYAAQE
                     LPDTIVRPDSLNQAELIADDSITLNGTNLWYDLSPDALDEMINYKGVDSIVYDLDSGK
                     TFIYAKGEITYQTFNLKADFIEFDWDAKTICAKQITDSTGKKGEKVFFKDGDEEFNAE
                     YMCYNFETKKGKIYYFRAQEGEGYIQVEAAKMTGDDSYYGDHLSYTTCELDHPHFYIA
                     ADKAKVIPQKVAVTGPANLVIADVPTPLFLPFGIFPIKRGQTSGLIIPTYGKHFTQGY
                     FLRNGGYYFALNDYFDLALTGDIYSRGSWGLHAATAYRLNYKFSGNFRVDYAVNKLGL
                     PFALDYSENSGFYISWNHAQDGKARPNSSFSANVNLSSSDYLQNNAYNSSYLTNTLNS
                     GISFSQAFPSTPFNLTVALRHSQTISDNMVNLTLPEASLSMNRIYPLKKLSNKKEGFL
                     NQFSVTYNLKTRNEIISPDSLLFMPETLDKMENGMQHTVNSSAPLKFLKYFTFNPYYN
                     YTENWYLESIRKNYQLTIVTDTFPTGTGEDSITIDTVYLDIDTVNGFVAARYFNMGAS
                     INTKIYSTIQFNGKLKAIRHVMTPSLTFNYTPDFGAQKWGYYGDYYTAPTAEEPTPYS
                     IFEGSIYGGPSRGEVGSIGLNLSNTLEMKVYSKKDSVKHEKKIKILESFSFGTSYNLA
                     ADSLNLADISFSAYTTLFKNVRINFSGSFDPYILDSTGRNLNQFEWDVNQRLGRFNGG
                     NVSLSTDFQSKRNSNPTLATSAGTEGEREMVWNNPEGYIDFEIPWNFSISYNLRVTNA
                     PTPDGRDSLYTTQSAYFQGDLNLTPNWKVLLSSGYDLELKQFTYTSIDIYRQLHCWEM
                     GFKWIPFGARQSYIFNINVKASVLQDLKLTRKKEWTEF"
     gene            9377..10195
                     /locus_tag="PLB46_04335"
     CDS             9377..10195
                     /locus_tag="PLB46_04335"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRF75775.1"
                     /translation="MKKRTGKTSLLIGMFVVAFIVLTSFKSFSLSPVAIQPYKLKTVV
                     IDPGHGGHDHGCSGSKNNEKTVALNLSLKLGELIEKNYPDVKVVYTRKTDVFIELHER
                     AAIANRNNADLFICIHCNANPSSSPFGTETYVMGLHKTEANLNVAKRENDVILLEDNY
                     NQHYDGFDPSNPASHIIFSLNQHAFMEQSILFASSVEKYMKSHAKRSSRGVKQAGFLV
                     LWKTGMPSVLIETGFLTNANEEKFLGSSEDRLGEWRVKIYLTTQLTEFLYLAQA"
BASE COUNT         3206 a         1877 c         1878 g         3244 t
ORIGIN      
        1 gtttattatc ccaaaaaaaa tcaccctcca aaattgaaga gtgatttatt atggtatgaa
       61 aacaaactta taacttcaca aaatttttcg aaattgtttt tccttctgca tttattttaa
      121 tcacatatgc acccggaaca agcggactta catctaaggt tactgatgca ttcgcactaa
      181 ctaattgtgc taagccaaca cctgtgttat tgccgaaaat atcaaacact gcaatttgtg
      241 ctaaactagc ttctttagta tgaaatttaa cagtcaataa atcatgcaca ggatttggga
      301 aaatatccac ttgtaattta cctgcagcgt tgattattcc acctgcaatt tttaattcag
      361 gatttgttgt tgctgcaata ttttttactg caaggccata tccaattgca cccatatcgg
      421 tagttgcacc tgtagttaaa tctaaggcat ataagcggtc gaaagtggca acaccggtat
      481 tcgctgttaa ataggcgata ttcattccat ctttatataa aatatccata tcggttgttg
      541 ggtcagtcaa attttgcatg ataccacttg ctccaattgt aattaaagca ccatcgtttg
      601 gtgggttttg gttacttatt acatttaaca tctggtcgta atcataaagt gaggttgtaa
      661 gcgttcctgc aaaactattg gtatacgcca ccgcaccaat cattgggtct gctccgaaat
      721 taatatcagc agcaccataa tttaaattga ggtctgtgaa aaataaagta ccatctgtat
      781 ttaatctgta gttcgcattg tttgctccaa ctaccctgat tttatctaca acgggattaa
      841 aatccattcc aatattgttt ccacctaaca ttaaaatagt aggtacggca tttacaactg
      901 ttgctgcacc tgtgctaaca ttaatagtat acaattgtga ttcgttggtt gctgaattat
      961 atcctaaagc atataaagcg cctgttgcag gacggaaatc cattcctaca atcgcctgat
     1021 ttaacgtaat accggttaca ggcacagcct tacgaataac ggaagggtta ttactatcat
     1081 atgataataa aaaattattg gtatttaatg catacattaa attacccatt aattcaggat
     1141 aacttctatc tataaaacaa gcaatatcat caatgtataa accaccgcct atattggtag
     1201 taattaatgt tccaacaccg gttgttaaat caactgaaat taaacggtcc gcttcaagat
     1261 tggaattaac ggttaagtat gttttgttag tttgactaac tgcatcataa aaaatatcca
     1321 tgtctgcaga agaaaatgat ttacttactg ctccacctaa atttccaatg gtatttaaag
     1381 ttccattgtt tggcggatct tgtttagcca ccacttttaa taatgcatca acattatata
     1441 aaacagttga agttgaacca acataactat tagtatatgc accagttgcg atataaggat
     1501 ttgctcccgc attcacatca gctactgcat aatttaaatt aatatcagta aatgcaatgg
     1561 ctccggtaat aggatttaat cgataattgt aatcgcgatt gctggttaca cgaattctat
     1621 ccactgtagg attaaaatca aaatttacat ctaatctgtc tgcaccaaca ggtcctaaaa
     1681 ccaaagaaat aggtgcaata tttaccggcg ttgcaattgc agttgctata tcaactgtat
     1741 aaatttgggc catttgagtg ttacggtcgt agcctagcgc atataacata ccgtttaaag
     1801 ggcgaaaatc cattccttca atggtttgcc ccggagtaat accgctaata ctcccgtaaa
     1861 aatttgccga ttccggcata cctgcatcaa tactatacaa gcctccaccg cttaatgcat
     1921 aaatagtttg tgcttcggat tgtaaactta atccaactgc aagtgccatt gaaaataatt
     1981 tcattttagc tgttgcagac tgttttcctt tgagtaattt tttgagttgc atacaataac
     2041 gtttaattgt taaataaata tgttttactt gttaagtgat gatagtcatc acaactgcct
     2101 gctatttgtt taaaaccatt atatgagcgg atatacgcaa ttgcttcgtc tttggatttt
     2161 tatcctggat tttttttcaa tctttcagtg taaacagaac agtatggcat gaaacacctg
     2221 caattccatc gaaattttag tagttttacg cggtgaaaat ttcgcgcgtt ttaatcctgg
     2281 ttgttatact tgtagaaggg ctgatggcta atgcccaggc ttctttcgac cataaagtgg
     2341 ttgaagtggg caatatgggt ttttatgtca caaatgcgtt aaccctgggt cggcctgatg
     2401 tgcgcaattc accttccggt gagccatcta tggaatatcc gctgaattcc ggaattgagc
     2461 acctttttga agccggttta tggataggtg ctatagttga tggccagtat ttagtttcta
     2521 cctcagcaaa agatgctgca agcggatata ctaccggtca aacaggattt gagttttcag
     2581 cagaaatagg aaataccata caacaacgca gctcattaac cgcaagtgat ttttttaatt
     2641 tcgatgccgt ttctcatgct gatatgttaa ttgatgcttc agataaaaat gtgattgttc
     2701 ccggaaccac aattgctatt tctgagcata cacaaccgtt aaatgcagat gtgcatttag
     2761 aatcttatgc atggaattat tcttttgccg attattttgt gttattaaat tatagcatca
     2821 ccaataattc aaccaatatc tgggatagtg tttggtgtgg gttatggagc gatttagttg
     2881 tccgcaatgt aaatgtatct accgattttg gttctgcatt ttttagtcag ggtggttatg
     2941 gatatttagc cgattatttt actaattatg cttttgatgt agatggcgat ccgggattta
     3001 ccaattcgta tggtgcattt caatttttag gcatcgaatg gcgcgaccag tttattcatc
     3061 caaataataa tgtcaattta attgctgccg gattaccagc tgcaaatgtg aatagtaatt
     3121 tttggatttt taattcaacg gcaacacctc cttataatgc acctgcaaat gatgttgaac
     3181 gctatgataa attgcaagac ggattaaact atgctgatcc gggcattgta gattttttac
     3241 atgacccgtc cacaactggt gggttaacaa atttattatc tgctggacct gtaattgcca
     3301 ttgaaccggg tgaaactatt catttagttt ttgctgctgt ctgcgccaaa caaatcgaca
     3361 atggtggtgt aaacggcgaa gaaaaagata cgcctgaagc acaagctaca ttagtagacc
     3421 atttaagctg gtcgaaacgc acctatttag gagaagacca aaatgagaat ggtattttag
     3481 atgcaggtga agatttagat gcggatgata ttttagaccg atatatttta cctgaacctc
     3541 ctgctacgcc aaaaattaaa gtgattgcta atagtcagtc aattgaaatt tattgggaca
     3601 acaaagcgga gttttcggtt gacccaattt caaaagaagt tgattttgaa ggatatcgtt
     3661 tatacagaac tcagcccggt gacgatttta atttaaattt attaggcgat gccaatatga
     3721 ttgctcaatg ggatttgccg ggtaataatt tgggttataa taatggatta caacttgttg
     3781 ctttaacaac gcctgaaata attgatgcag atacctttta ttacaaatac actttagaca
     3841 atgtgttaaa tggatggcaa tatttaatta tcctaaccgc atttgatcgt ggtgatgaaa
     3901 atttaaatat cgaaagttta gaatcttctt ttattgaaaa tgcggtgagt gtttttccgg
     3961 gtactttagc cacttcggac gaacaaactg ctattggtgt ttatccaaat ccatatcggt
     4021 taaatgccgc ttgggatgga gcaacatcaa caaccagaaa aataattttt tatcatttgc
     4081 ctgcaaaatg tgaaatcacc atttttactt tagggggaga tattgtcgct actattcaac
     4141 acgatggtga caattataat ggctccgata ttggttggtt tgatacgtat ggtggtaatg
     4201 aaaatgcgcg tgagttttcc ggcggtgaac acgcctggga tattttaagt gaaagtaaac
     4261 aaaccattac gcaaggtatt tatttattta atgtaaaaga ccttgaaacc ggcatcaaca
     4321 aacaaggccg ttttgttgta atagaataag ttttccattt tgtttagtca aaaatgctat
     4381 ttaatattcg acaatactta aattgttagc ctatctttgt aaaaaactta catgtccgcg
     4441 ctaacgtcgt atcctaaaga aaaaattcag attctgcttt tagaaaatat agctgacaga
     4501 gctgttcagg ggtttgtcga ttccggatat gcacaagtga aaaaattaag tggggcatta
     4561 gaagaagacg cactgattga tgctattaaa gatgtacatt tattaggcat ccgttcaaaa
     4621 acactaatta cttcgcgggt tttacaacaa gcaaataaat tgctggcggt aggatgttat
     4681 tgcattggca ccaatcaggt tgatttacaa acagcaaaag aaaaaggtgt tgtagttttt
     4741 aattctcctt atccaaacac acggtctgta gcggaattag taatagctga agccatcatg
     4801 ttattgcgca gaattccgga aaaaaacact gccgcgcatc agggtatttg gctaaaagat
     4861 gcccgcaatt gttttgaatt acgtggaaaa acactcggca taattggata tggtaatatt
     4921 ggtatgcagg tatctgtatt agcagaagct ttgggaatgc gggttatata tactgatgta
     4981 actacaaaat tgccaatggg taacgctcaa caaattcatc agctttatga cttgttaaaa
     5041 atagcagatg tagtttcgtt acacgtgccc gaagcgccat ctactattca attgttgaac
     5101 gatgctgcgc tttctcaaat gaaaaagggc agtattttaa taaattgtgc cagagggaat
     5161 gtaattgatt tgaaggcatt aaaaacacat ttattatcag gccatattgg tggagctgca
     5221 attgatgtat ttcccgaaga accggaaaaa aacggagctg aatttgtttc ggatttacaa
     5281 caaatcccta atacaatatt aaccccgcat attggtggct ccacggaaga agcacaggta
     5341 aatataagcg aagatgttac agctaagctg attggatttt tggaatccgg tgttaccagt
     5401 ggctcacata cagtaccagc attacattta ccccaacatc ctaacgctca ccgtattttg
     5461 catattcacc gcaatacacc tggtgtttta tcagcaataa acagcgtttt ttcaaacaac
     5521 gaagtgaata tcgttggtca atacctcaac accaatactg aaattgggta tgctgttata
     5581 gatgtcaacc ggaatttaac cacctcaact atagaggcat taaagaatgt gcctcatacc
     5641 attaaaatgc gggttgttta ttaataactt ttgtgtacgg ccttgtaata aaatcttact
     5701 catggtaaca ttagtttgat aaaagacact taaatttata ccgaacaaac actaattaca
     5761 tgaagcctat ctattcaacg acactcgctt tatccctttt tattacattt aaccttaacg
     5821 cagctatttc caatttttgt tttgtggatg aattcgggta tattgctgaa gtcactgcaa
     5881 caagaacagg tccgggctat tacgaattaa acggaactgc agatgtattt gcaggttacg
     5941 actggattgt gacaggttat tatgataaaa catctgatgt ttggagttta acttacacga
     6001 acccattccc tgatggatgt ttagtattta ccgattattt tgtttacact tctacctctc
     6061 acactgccgg aaatattaat ttcgactgga caaattattg tttcggtggg gcaacagatg
     6121 tgggaagtgc cagcaccatt tatttcaaat ctttatgtgt tttccgatta cctgagtcca
     6181 ctcctgccgg cccggcaagt gctgataaat cattatcagg tataaaacaa tttatcgcca
     6241 ctccgcaaga aggaactccg cttttccgcg atttattatt cgatcaggaa tttagtgtaa
     6301 caactacggc tgaaaataat tatactatca gttttgaatt agataattca actgatgtta
     6361 aaattgatat ttataattat gccggtcaat ttgttacaac tgtaactgaa gctgcataca
     6421 atagtggatt ttacaaacta aattggaacg gaaatgatgc tgcaggaaat gaaagtaaac
     6481 caggtatgta tttagctata ctacattcag acaatgagca actatcgtat aaattcatta
     6541 aataattgaa acaaaattta ttgaaaaaag tgcgaataat ttcgcgcttt ttttttgcaa
     6601 taaagttggg aattctgacc tcaaaattct gtccactctt ttttccgggt aagttttaaa
     6661 tcctggagca cacttgcttt tacattgata ttaaatatat aactctgtct agctccaaat
     6721 ggtatccatt taaagcccat ttcccaacaa tgtaattggc gatagatatc aatactggtg
     6781 taagtaaatt gcttcaactc tagatcgtat cccgacgata ataatacctt ccagttaggc
     6841 gttaaattta aatcaccctg aaaataagcc gattgtgtag tatataaaga atctcttccg
     6901 tcaggtgttg gtgcattagt tacacgtaaa ttgtaggaga tgctaaaatt ccatggtatt
     6961 tcgaagtcga tatatccttc agggttattc caaaccattt cccgttcacc ttcagtaccc
     7021 gctgatgtag ctaaggtggg attagaattt cgtttagatt gaaaatcggt actcaaactc
     7081 acatttccac cattaaatct tcccaggcgt tgattaacat cccactcaaa ctggttgaga
     7141 tttcttcctg ttgaatccaa aatataaggg tcgaaacttc ctgaaaaatt gatacgcaca
     7201 tttttaaata aagtagtata tgcgctgaat gaaatatcag ccaaatttaa agaatctgca
     7261 gcgaggttat acgaagttcc aaagctgaaa ctttctaaaa tttttatttt cttttcatgt
     7321 tttacagagt ccttcttcga atacactttc atctccaatg tattactcag atttaaccca
     7381 atactcccta cctcgccccg cgagggacca ccataaatac tcccctcaaa gatgctatat
     7441 ggggtgggct cttctgctgt aggcgctgta taataatcgc cataatatcc ccacttttga
     7501 gcaccaaaat caggtgtata attaaaggtg agggatggtg tcattacgtg tctgattgcc
     7561 tttaattttc cattaaactg tatggtagaa taaattttag tattaatact ggcgcccata
     7621 ttaaaataac gagcagcaac aaatccattt acggtatcaa tatctaagta aacggtatca
     7681 atggtaatac tatcctcacc tgttccggtg gggaaggtat cggtaacaat agttaattga
     7741 taattttttc gaatagattc caaataccag ttttcagtat aattataata tggattaaac
     7801 gtaaaatatt ttaaaaattt taaaggcgca gaagaattaa cagtatgctg catgccgttt
     7861 tccattttgt ctaatgtttc cggcataaat aaaagtgaat cgggcgaaat aatttcgttt
     7921 cgtgttttca gattataggt aacactaaat tgatttaaaa aaccttcttt tttattactc
     7981 aattttttta acggataaat gcggttcata ctcagcgatg cttcaggcaa agtgaggttt
     8041 accatattgt cggaaatagt ttgagaatga cgcaatgcaa cagttaaatt aaaaggcgta
     8101 ctagggaaag cctgtgaaaa tgagatacca gagtttaagg tattggttaa ataactgcta
     8161 ttgtaggcgt tattttgtaa ataatcgctt gagcttaaat tcacatttgc actaaatgat
     8221 gagttgggac gtgctttccc atcctgtgca tgattccatg aaatataaaa tccggaattt
     8281 tctgaataat ctagcgcaaa tggtaatcct aatttattaa cagcataatc taccctgaaa
     8341 ttaccactga atttataatt taaacggtaa gcagttgcag catgcaatcc ccagcttccg
     8401 cgtgaataaa tatcgcctgt taatgcaagg tcaaaataat cgttgagcgc aaaataatat
     8461 cctccattgc gtaaaaaata tccctgcgta aaatgttttc cgtatgttgg aataattaac
     8521 ccggatgttt gacctcgttt aatcggaaaa atcccaaatg gtaaaaataa tggtgtgggt
     8581 acatcggcaa taaccaaatt tgcagggcct gtaacagcaa ctttttgcgg aattactttg
     8641 gctttatcag cagcaatata aaaatgcgga tgatccaatt cgcaggtagt ataactcagg
     8701 tggtcgccat aataggaatc gtcaccggtc atttttgctg cttcaacttg aatatatccc
     8761 tctccttcct gtgcccggaa ataatatatt ttcccctttt tagtctcaaa gttataacac
     8821 atatattcgg cgttaaactc ttcatcgcca tctttaaaaa acactttttc accctttttt
     8881 ccggtgctgt ccgttatttg tttagcacaa attgttttag cgtcccagtc aaattcaata
     8941 aaatcggctt tcaaattaaa tgtctgatag gtgatttcac ccttcgcata aataaaagtt
     9001 tttcccgaat caaggtcata cacaatagaa tcgactcctt tataattaat catctcatcc
     9061 agtgcatcgg gcgaaaggtc gtaccataaa ttagtaccat ttaacgttat actatcatct
     9121 gcaattaatt ccgcctgatt aagtgagtct ggccgcacga tggtatcggg aagttcctga
     9181 gcagcataat tcacaacatt ttggttggat tgctcatttt tatatacaaa aatgccatcg
     9241 gatgccttta aaaacataca attcacaaaa atcgatatgg ctaaaagcag aaatttacgt
     9301 ggcacagata attttggaat aattttatta ctaaatggat gtttacttac aattattgtg
     9361 caaagctatt gaaattatga aaaaaaggac cggcaaaaca agtttgctaa ttggaatgtt
     9421 tgtggttgca tttattgttt taacctcatt taagtcattt tcgttatctc cggttgccat
     9481 tcaaccgtat aaattaaaaa cagtagtcat agatccgggt catggaggcc atgatcatgg
     9541 atgcagtggc agcaaaaaca acgaaaaaac agttgcactc aatttatcgc ttaagctggg
     9601 tgagctcatc gaaaaaaatt acccggatgt aaaggtagtg tatacgcgca aaaccgacgt
     9661 atttattgaa ttacacgagc gggcagcaat tgctaaccgc aataatgccg atttatttat
     9721 ctgcattcac tgtaacgcca acccaagttc atctcctttc ggcacagaaa cctatgtaat
     9781 ggggttacat aaaaccgaag ccaacttaaa tgtggccaaa agggaaaatg acgtaatctt
     9841 attggaagac aactacaatc agcattacga cggtttcgac ccgtcaaatc ctgcctcaca
     9901 tatcattttt tctctcaacc aacatgcgtt tatggagcaa agtatcttat ttgcctccag
     9961 cgttgaaaaa tatatgaaaa gtcacgcaaa acgcagcagc cgaggggtta aacaggccgg
    10021 tttcctggtt ttatggaaaa cagggatgcc gagtgtgttg attgaaaccg ggtttttaac
    10081 caatgccaac gaagaaaagt tcttaggttc atctgaagat aggttaggcg agtggcgagt
    10141 gaaaatttac ctaacgacgc aactcacaga gttcttatac ttagcccagg cctaaatgcc
    10201 tgggc
//
LOCUS       DAORKW010000065        10200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_142_length_10200_cov_5.534155, whole genome shotgun sequence.
ACCESSION   DAORKW010000065 DAORKW010000000
VERSION     DAORKW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142_length_10200_cov_5.534155"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..517)
                     /locus_tag="PLB46_04340"
     CDS             complement(161..517)
                     /locus_tag="PLB46_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75776.1"
                     /translation="MKHLSILVPDEQTNMSTVACIVGAFQVFSEANNYLIKKNEKGLF
                     KIELVGAQKNEYLINNLLSIKHQVTIDEINNTNLIIIPASLIRSYGTSSKNNQLLINW
                     VAQQYRLGAEVASMCA"
     gene            550..1116
                     /locus_tag="PLB46_04345"
     CDS             550..1116
                     /locus_tag="PLB46_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF75777.1"
                     /translation="MSISTRENVYFTPYLYPFWTLPLLSNIQTMVNTALFVKMVIDRW
                     NGSILSLNKNLSVLTDDQLHKAVAPGKNRGIYLLGHLIAVHDEMLVLLALGDKLYPEL
                     YEPFIKQADNINTQLPSAVELREMWEKQNTHLQAKFEQVQPEEWFGRHTAVSAEDFAK
                     EPHRNKLNIIISRTSHLQYHTGQIVLLN"
     gene            1126..1554
                     /locus_tag="PLB46_04350"
     CDS             1126..1554
                     /locus_tag="PLB46_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF75778.1"
                     /translation="MTNQHFQYSFTSSKNANEVFAFLINPKNWWMGLFDETIEGKSEN
                     VNDAFSFSAGGGLHNSNQKLITVIPGKKIVWLVTESNLSFLNNTNEWADSKICFEIEQ
                     NGIKTNITFTHIGLMPQIECYESCSSAWSQYMTNLQQHLK"
     gene            1629..2132
                     /locus_tag="PLB46_04355"
     CDS             1629..2132
                     /locus_tag="PLB46_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF75779.1"
                     /translation="MNKTSGNSYTTTILVDKDISTVFNAIKNFSAWWSEEIEGKTDIV
                     GETFFYHYKDVHLCKIKLIEMIADKKLVYQVIDNEFNFTKDKTEWINTHLIFDISDEN
                     GKTKIVFTHDGLTSAYECFQVCNDAWTSYIQGSLKNLIETGIGKPNGKEGGLNAELVE
                     KWGLPNK"
     gene            2661..4340
                     /locus_tag="PLB46_04360"
     CDS             2661..4340
                     /locus_tag="PLB46_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRF75780.1"
                     /translation="MIEKGNLNFIEEIIKTDLEAGKYTEIITRFPPEPNGYLHLGHAS
                     SICLNFGITQKFNGYTNLRFDDTNPITEETEYVESIIHDIKWLGFQWKNMYFASDYFD
                     KLYEFAVALIKNNLAYVDDSTSEEIASQKGTPTQAGIPNIYRSRTVEENLLLFQEMKD
                     GKYADGQKVLRAKIDLSSSNMLMRDPIMYRIKHAHHHRTGDKWCIYPMYDFAHGQSDS
                     IENITHSICTLEFAPHRELYDWFILKLNIFPSHQYEFARRNISYTVMSKRKLLQLVNQ
                     QIVDGWDDPRMPTISGLRRRGFPPESIVEFCNKIGIGKRENSVDISLLEYCVREQLNK
                     TAERRMVVLDPVKVTITNYEGEEEIQTENNPEVDVVKYRTMPFTSELYIEREDFLEIP
                     TKKFHRLTIGNYVRLKSAYIIQCNEVIKDSTGKIIELKCTYFPNSKSGNDTSELPAKG
                     VIHWVSSKYAIPVEVRLYDRLFMVENVDAAENGFLENINPNSLVIINNAFAEPILQSA
                     TLEDRFQFLRKGYFCLDKHSNNEKLIFNRTATLKDTWMNQPDKLTNKTDKN"
     gene            4368..6008
                     /locus_tag="PLB46_04365"
     CDS             4368..6008
                     /locus_tag="PLB46_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRF75781.1"
                     /translation="MTYSKELISLIFPNDLLSPEALEAQYPKRDIPHGAIVTRFAPSP
                     TGFIHIGGVYIAVLGKDLTFHSNGVFFIRIEDTDQKRKVDDSKTQFNTTFKYFGIESD
                     ENDSNSIYGPFTQSKRANIYLAYVKKLMEKNRAYPCFCTKEMLEEQTNEQKEAKVDLG
                     YYGKWAKCSRLSEQQIKQKIDAGEPYVIRFRCNGDDNTVEFEDKIKGTIGLKNNINDI
                     VILKSSSEETRLPTYHLAHPIDDHLMGVNLVLRADEWLPSVPLHFQLFDALEFERLPY
                     AHIAPIMKLDGSSKRKLSKRKDPEASVGYYMENGYPSKAVTAYLKSLANSRLLDATID
                     ECLSAQIMLDEMPKAGALLDITKLNHISSNYIATLTNKEIGLELKKWALAYDMDLYNV
                     IEKDFDYVCSAIDTDRINDGKIRKDLSKWSDYKKVFGYFHNELFNYLEITDERFENIS
                     IETIKQVLGDFALHYAENLENDSWMEVLQTVAKNAGFAINNQEYKKEPEKFKGKLSDA
                     IKIIRIALTGSANGLNLHEVCKTIGSMEIKNRISHVIN"
     gene            6023..6322
                     /locus_tag="PLB46_04370"
     CDS             6023..6322
                     /locus_tag="PLB46_04370"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75782.1"
                     /translation="MFCKYITYRDCDSRKLAAVKTAYINNIKPPNTVVLSTCHRIEFY
                     SQDAFDLHEPPKDLTGFWNSLEGIEDILIRLATIACGADSKILGENFIAFQTLSK"
     gene            complement(6337..7335)
                     /gene="dusB"
                     /locus_tag="PLB46_04375"
     CDS             complement(6337..7335)
                     /gene="dusB"
                     /locus_tag="PLB46_04375"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRF75783.1"
                     /translation="MPSIGNITLPDFPILLAPMEDVSDPPFRVLCKQLGADLMYTEFI
                     STEGLIRNAEKSLKKLDFYEEERPIGIQIFGADIEVMKGCAEIVEQAGPDLLDINFGC
                     PVKKVVCKDAGAGILKDIPRMVRLTAEIVKATKLPVTVKTRLGWDDNSKNIMEVAERL
                     QDIGIKALTIHGRTRAQMYKGEADWTLIGDVKNNQRIQIPIFGNGDVDTPQKALEMKN
                     RYGVDGIMIGRAAIGAPWVFREIKHFLATGEELPPPTISERVDICRRHLIKSIEWKGP
                     VLGILEMRRHYTNYFKGIPNIKSYRNQLVTVNSLEEIDEVLLEITRLTNDNVEVGV"
     gene            7375..8403
                     /locus_tag="PLB46_04380"
     CDS             7375..8403
                     /locus_tag="PLB46_04380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRF75784.1"
                     /translation="MPAQMLWFTYFYSKFSFHLKGSIKYLPWVGKLLVLLGIYLIVEY
                     IFALMAVEIINAIFPGMDFLHFITGFEKIKSVQEVTLDQEHAIKIYQFATSLGRFIFV
                     AWFFIYLCGDNFLASVSLNKNIQAVSYGTVILIVMASGAIISLIHDWNQHLHLPASMA
                     EMESQMRSLEQQAKIQTEVFLRTTTTGGFLLNILIIGLLAAVGEEIFFRGVLQNIFFR
                     GTGNAHIAIWSAAFLFSFIHFQFFGFFPRLLMGALLGYLYYYSGSLWSAILAHFVNNV
                     ATVIAYFLLNKGIITENVTETVNVWAALIAIPVVFLLIRYFKKTETQLSFAVAKKQED
                     NITLDGIQ"
     gene            8435..9274
                     /locus_tag="PLB46_04385"
     CDS             8435..9274
                     /locus_tag="PLB46_04385"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRF75785.1"
                     /translation="MNNLVLRIVTALIGVAVIIGSIFLNTWVFVLVFMAIGFATHFEY
                     MRTITLNASKKPGIELPFALAAGLAIITAINANVFDLSAIHLLSVAVFLWPAGIIILE
                     IFYNREKPFEHIGLTILGLLYIPLAFGLFIFYAIDFGGTVKTWFAVGLLFMVWFNDSF
                     AYFAGRFWGKRKLFERISPKKTWEGFWGGLVMTIVCGVIMSFLFDELGLVQWMVWGLI
                     VSVSGTLGDLAESLLKRSIQIKDSGSVLPGHGGFLDRFDGFIMVIPFTVAYLEILRFF
                     HLV"
     gene            9345..10022
                     /locus_tag="PLB46_04390"
     CDS             9345..10022
                     /locus_tag="PLB46_04390"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HRF75786.1"
                     /translation="MLRLIHKEGRKIIIAFLAFLVALNFATHYFLPEQGLFNTLILVA
                     SLGGILFVTMFFRNPKRRLFTEEGAIIAPADGKVVVIEETTENEYFKDRRLQVSIFMS
                     VKNVHINRTPISGQVKYFKYHPGKYLLAWDPKSSTENERTTYVIEDEDDNMEILMRQI
                     AGTVARRIKFYVEEGDEVTQGAEFGFIKFGSRVDLFLPLDSKIEVNIGEKVKGGQTVI
                     ARVARTK"
BASE COUNT         3413 a         1786 c         1885 g         3116 t
ORIGIN      
        1 aataggcgcc accattggtg tagataccgt tttcatcggt aatcaactgg tcggtttgta
       61 agtgcacatc aggaaacaaa gtcctaaaat tttctgctaa agcccaatgt gtagagcaag
      121 ttttacccga taaaataccc gtagatgcca acataaaact tcacgcacac atactcgcaa
      181 cttcagcacc caacctgtat tgttgcgcta cccaattgat taaaagttgg ttgttttttg
      241 atgaagtacc ataactgcga atcagcgatg ccggaataat aattaaatta gtattattta
      301 tttcatcaat ggtaacttga tgttttatag aaagcaagtt gttaattaaa tattcatttt
      361 tttgagcacc aaccaactca attttaaaca gacccttttc attttttttg atcaaataat
      421 tgttcgcttc actaaaaacc tgaaatgctc caacaataca ggcaacagtg ctcatattag
      481 tctgttcgtc cggaaccaaa atactcaaat gtttcatttc cgctttttga acaaaggtaa
      541 acaaacctat tgtccatatc aacccgtgaa aatgtctatt ttacacctta tttataccca
      601 ttttggacat tacctttgtt atcaaatatt caaactatgg taaatacagc attatttgta
      661 aaaatggtca tcgacagatg gaatggctca atactgagtt taaacaaaaa tcttagtgtt
      721 ttaactgatg atcaattaca caaagcagtc gcaccgggta aaaacagggg aatatatttg
      781 ttggggcatt tgatagcagt acatgatgaa atgctggtgc ttttggcact cggagataaa
      841 ttatatcctg aactttacga accttttata aagcaggcgg ataacataaa cacacaactt
      901 ccatctgctg tcgagttaag ggaaatgtgg gaaaagcaga atacgcatct gcaagccaaa
      961 tttgaacaag tgcaacctga ggagtggttt ggaagacata ctgcagtatc agcagaagat
     1021 tttgcaaaag aaccacatcg caataaactt aacatcatca tttcgagaac ttcacattta
     1081 caatatcata cggggcaaat tgttttactc aactaaaaaa atgttatgac aaatcaacat
     1141 ttccaataca gttttacttc ttcaaaaaac gcaaacgaag tatttgcctt tttaataaat
     1201 ccgaaaaatt ggtggatggg tttatttgac gaaaccattg aaggaaaaag tgaaaatgtt
     1261 aatgatgcat tttcttttag tgccggagga ggattacata attccaatca aaaactgata
     1321 acagttatcc ccggtaaaaa aatagtttgg ttagttactg agagtaattt gtcattctta
     1381 aacaacacca acgaatgggc agattcaaaa atttgtttcg aaattgagca aaatggtatt
     1441 aaaacaaaca taacctttac gcatattggg ttaatgccac aaattgaatg ttatgaaagt
     1501 tgttcaagtg cctggtctca gtatatgaca aatctgcagc aacacctcaa ataataattt
     1561 aaactattgt actcgtagca ttgctttatt gcagtgaata aaatcaataa gaaactcaat
     1621 taaaaaaaat gaataaaaca tccggtaata gttatacaac taccatttta gttgataagg
     1681 acatttctac ggtatttaat gctatcaaaa atttcagcgc ctggtggtct gaagaaattg
     1741 agggtaaaac agatatagtc ggcgaaacct ttttttacca ttataaagat gtgcatttat
     1801 gcaaaataaa actcattgaa atgatagccg ataaaaaatt ggtttatcag gtaatagaca
     1861 atgaatttaa ttttacaaaa gacaaaactg aatggataaa tacacacctg attttcgata
     1921 taagtgatga aaatggtaaa actaaaatag tatttaccca tgatggttta acatctgcct
     1981 acgagtgttt ccaagtttgt aacgatgcct ggacaagtta tatacaagga agtttaaaaa
     2041 atttaattga aacaggtatc ggaaaaccaa acggaaaaga aggtggatta aatgccgaat
     2101 tggttgaaaa atgggggctt cctaataaat aaaaaaacgt ccccttatca agatttttaa
     2161 aatgtttagc tgaagtttca atttaaacga atgtttattc gagttacttg tatgcaaaaa
     2221 ccggtgaaat tggccactcc acaccgattt agacggatga ttagaatttc ataaatttaa
     2281 agtatcaact actacaaatt ctattgcaac agactttatt aagctatcaa atgacaaaaa
     2341 tttaccggct aaataaagaa tggtaaatat ctgctccatt aagggcttgt atttaaacaa
     2401 gccgactggc ttaagaacga aaattgcatg tgctaatttg cacctttgcg cctattgagc
     2461 gaaatcaaaa tccagcatca aattccgaaa tccaaaattt agcatttgaa tgaaagattt
     2521 ttttctactc tttcatagtc gcaaggccgc aaatcactag gggagccacg ctttttatac
     2581 cttttactaa aagtaaaaag tgaaaccgtt caaacaaaaa gaatcactaa ctttgccata
     2641 acactataaa gcaataaaaa atgatagaaa aaggtaactt aaactttatt gaggaaatta
     2701 ttaaaactga tttagaagcc gggaaataca ctgaaattat aaccagattt ccgcctgaac
     2761 ctaatggtta cctgcatttg ggtcatgcaa gcagtatttg tctcaacttt ggaataaccc
     2821 aaaagtttaa tggttatact aatttacgat ttgatgatac caacccaata acagaagaaa
     2881 ccgagtatgt cgaaagtatt atacatgata ttaaatggct gggttttcag tggaaaaata
     2941 tgtacttcgc aagtgactac tttgacaaat tatacgaatt tgcagtagcg ctgattaaaa
     3001 ataatttagc ctatgtagat gatagcacca gtgaagaaat tgcatcacaa aaaggcacgc
     3061 caacccaagc cggaataccg aatatttata gatcaagaac tgtagaggaa aatttattgc
     3121 tattccaaga aatgaaagac gggaaatatg ctgatggaca aaaagtatta agagcaaaga
     3181 tagatttatc ctcctctaac atgttgatgc gagaccctat tatgtatcgt ataaaacatg
     3241 cccatcacca tcgcacaggc gataaatggt gcatttatcc aatgtacgat tttgctcatg
     3301 gacaatctga ctcgattgaa aatattacac atagtatttg caccttagaa tttgcccctc
     3361 atcgtgaatt atacgattgg ttcattctta aactaaatat tttcccttct catcaatatg
     3421 aattcgcaag gagaaatatt agttacaccg taatgagtaa acgcaaatta ttgcagttag
     3481 tcaaccaaca aattgttgat ggctgggatg atccgcgaat gccaactatt agcggcctaa
     3541 gaagacgtgg tttcccgcca gaaagtattg tagaattttg caataaaata ggtatcggga
     3601 aacgtgaaaa ctcagtagac attagtcttt tggaatattg tgtaagagaa cagctaaata
     3661 aaaccgctga aagaagaatg gtagttttag atcctgtgaa ggtgacgatt acaaattatg
     3721 aaggtgaaga agaaattcaa actgaaaata atcctgaagt agatgtagtt aagtacagga
     3781 ccatgccatt tacgagtgaa ttatacatag aacgtgaaga ttttttggaa attcctacta
     3841 aaaaattcca ccggttaact attggcaatt atgtaagatt aaagagcgct tacattattc
     3901 aatgtaatga ggtaattaaa gattcaacgg gtaaaatcat tgaattgaaa tgcacctatt
     3961 tcccaaacag caaaagtggt aacgatacta gtgaactgcc agcaaaaggt gtaattcatt
     4021 gggttagcag caaatatgct atacctgtag aagtacgact ctacgatagg ttgtttatgg
     4081 tggagaatgt tgatgctgct gaaaacggat ttctcgaaaa cattaatccc aattctttag
     4141 taatcataaa caatgctttt gcagaaccta ttctccaatc tgcaacgttg gaagatagat
     4201 ttcagttttt acgcaaaggg tatttttgtt tagataaaca tagcaataat gaaaagctta
     4261 tttttaatcg aactgcaacg cttaaagaca cgtggatgaa ccaacctgat aagttgacaa
     4321 acaaaactga taaaaattaa taaggaacaa acatcaattt tcggcacatg acatatagca
     4381 aagaattaat ttcacttata tttccaaacg atttactaag cccggaagca ctagaggcgc
     4441 aataccctaa aagagatatt ccacatggag caattgttac aagatttgca ccaagtccca
     4501 caggttttat ccatattggt ggagtataca ttgcggtttt aggtaaagat ttaacctttc
     4561 attctaatgg cgtatttttt attagaattg aagataccga ccagaaaagg aaagtagatg
     4621 attcaaaaac acaatttaat acgactttca aatatttcgg aattgaatct gacgaaaatg
     4681 attcaaattc gatttacgga ccttttacac aatctaaaag ggcaaacatt tatcttgctt
     4741 atgtaaaaaa attaatggaa aaaaacagag cttatccatg tttttgtact aaagaaatgc
     4801 tggaggagca aaccaatgag caaaaagaag caaaagttga ccttggttat tatggaaaat
     4861 gggcaaaatg cagcaggctg tcggagcaac aaataaaaca gaaaatagat gcaggcgaac
     4921 catatgttat tagatttaga tgcaatggtg atgataatac cgttgaattt gaagataaaa
     4981 taaaaggcac tattggtctt aaaaacaata ttaatgatat tgtaatcctt aagtcgtcat
     5041 ctgaagaaac ccgactgcca acctatcatt tagcacatcc tatagatgac cacttaatgg
     5101 gagtcaacct agtactcaga gcagacgaat ggttgccctc agtgccactt cattttcaat
     5161 tatttgatgc tttagaattt gaaagattac cctacgcgca cattgcacca atcatgaagt
     5221 tagatggtag ttcgaagcgt aaactctcta aaagaaaaga ccctgaagct tcagtaggct
     5281 attatatgga aaatggctac ccttctaaag cggttacagc ctatttgaaa tcattggcaa
     5341 actcacgatt attagatgcc acaatagatg aatgtttatc agcacaaata atgctcgacg
     5401 agatgcctaa agcaggtgca ttacttgata ttaccaaatt aaaccatata tcctcaaatt
     5461 acatagctac tttaaccaat aaagaaattg gtttagaatt aaaaaagtgg gccttagctt
     5521 atgatatgga tttatataat gtaatcgaaa aagactttga ttatgtgtgc tcagcaatag
     5581 atacagatag aataaacgat gggaaaatta gaaaagattt gagtaaatgg tctgattaca
     5641 aaaaagtatt tggctatttc cacaatgaac ttttcaatta cctcgaaatt accgatgaac
     5701 ggtttgaaaa tatttcaata gaaacaataa aacaagtgct tggagatttc gctttgcatt
     5761 atgctgaaaa tctggaaaat gatagttgga tggaagtact tcaaacggtt gccaaaaatg
     5821 caggttttgc aattaacaat caagagtata aaaaggaacc ggaaaagttt aaaggtaaat
     5881 taagcgatgc cattaaaata atacgcattg cccttaccgg tagtgctaat ggactaaacc
     5941 ttcatgaggt ttgcaaaact attggcagca tggaaattaa aaatagaatt tctcatgtta
     6001 tcaattaaaa atattataaa ccatgttttg taagtatatt acatacaggg attgtgatag
     6061 caggaaatta gccgctgtta aaacagcata tataaacaat attaaaccgc ctaatactgt
     6121 agttttatct acttgccatc gtatagaatt ttattcacag gatgcatttg atttacatga
     6181 gccgccaaaa gacctaacgg gattttggaa ttcccttgaa gggattgaag atatactcat
     6241 cagattagca accattgcat gcggtgccga ctctaaaatt ttgggggaga attttatcgc
     6301 atttcaaact ttatctaaat aacaacacaa aaaaaattac acccctacct caacattgtc
     6361 gtttgtgagt ctagttattt ccaataatac ttcatcaatt tcttctaacg aatttacggt
     6421 taccagttgg ttgcggtatg atttaatatt cggaatacct ttaaaataat tcgtgtagtg
     6481 tctgcgcatt tctaaaattc ccaataccgg ccctttccat tctatagatt taattaaatg
     6541 tctgcgacaa atatcaacgc gttcgcttat tgtcggtgga ggtaattctt caccggttgc
     6601 cagaaaatgt tttatttcac gaaacaccca aggagctccg atagctgcgc ggccaatcat
     6661 aatgccatct accccataac ggtttttcat ttccaatgct ttttgaggcg tatctacatc
     6721 gccgttgcca aaaataggaa tttgaatgcg ctgattattt ttaacatcac caatcaaggt
     6781 ccagtctgcc tcacctttgt acatttgcgc acgcgttctg ccatgaatgg taagcgcttt
     6841 aataccaata tcctgcagcc tttctgccac ttccataata tttttggagt tatcgtccca
     6901 accaaggcgg gttttcaccg taaccggcaa cttagttgct ttcacaattt cggcagtgag
     6961 gcgcaccata cggggaatat cctttaagat acctgcgccg gcatctttac acaccacttt
     7021 ttttaccgga caaccaaagt taatgtccag caaatcgggg ccggcctgct caacaatttc
     7081 ggcgcaaccc ttcattactt ctatgtctgc cccaaaaatc tgaatgccaa ttggtcgctc
     7141 ctcttcataa aagtcgagct ttttcagact tttttcagca ttgcgtataa gcccttctgt
     7201 ggagataaac tccgtataca tcaggtctgc ccccagctgt ttacacaaca ccctaaaagg
     7261 cggatcgctc acatcttcca taggtgcaag caaaatgggg aaatcaggca atgttatgtt
     7321 accaatactc ggcatagccc aacaaagtta gccattagta gccaaagccg gggcatgcct
     7381 gcacagatgc tttggtttac ctatttttac agcaaattca gtttccattt gaaaggcagt
     7441 ataaaatatc tcccatgggt aggcaagtta ttggtgttgc tgggtatata ccttatagtg
     7501 gaatatatat ttgctttaat ggcagtagaa ataatcaatg ccattttccc cggcatggat
     7561 tttttacatt ttattacagg tttcgaaaaa attaaatccg ttcaggaagt tacacttgac
     7621 caggaacatg ccattaaaat ttatcaattt gctacttcat tggggcgttt tatttttgtt
     7681 gcctggtttt ttatttattt atgtggagat aattttttag caagtgtttc gctgaataaa
     7741 aatatacaag cagtttcata cggcacggtg atattaattg taatggcttc cggtgcaatt
     7801 atctccttaa tacacgattg gaatcagcat ttacatttac ctgcttctat ggcagaaatg
     7861 gaatcgcaaa tgcggtcgtt ggagcaacag gcgaaaattc aaacagaagt atttttacgc
     7921 accaccacta caggaggatt tttattaaac attttaatta ttggattatt ggcagcggtt
     7981 ggtgaagaaa tatttttcag aggtgtatta caaaatatat ttttcagagg aaccggcaat
     8041 gcacatattg ctatttggag cgctgcattt ttgttcagct ttattcattt ccagtttttt
     8101 ggatttttcc cacgtttatt gatgggagcc cttttggggt atttgtacta ttacagcgga
     8161 agcctctgga gtgccatact ggcgcacttt gtaaacaatg tagctactgt aattgcctat
     8221 tttttattga acaagggtat tatcacagaa aacgttactg aaaccgtaaa cgtttgggct
     8281 gctttaatag ctattccggt agtattttta ttaatacgtt atttcaaaaa aacagaaaca
     8341 caattgagtt ttgctgttgc gaaaaaacaa gaagataata tcacattgga tggcattcaa
     8401 taacacaaaa tcaagttaat tgtaatttac caaaatgaat aatttagttt tacgtatagt
     8461 aactgcttta attggtgtag ctgtaattat tggcagtata tttttaaata catgggtttt
     8521 tgttttggta tttatggcca ttggtttcgc tacgcatttt gaatatatgc gaactattac
     8581 gctaaacgca agcaaaaaac ccggcattga attgcctttt gctttagcag caggtttggc
     8641 aataattact gcaattaatg ccaatgtttt tgatttatct gcaattcatt tattaagtgt
     8701 agctgttttt ctttggcctg caggtattat tattttagaa atattttaca accgcgaaaa
     8761 accatttgaa catattggtt taactatttt agggttgtta tatatcccgc tggcatttgg
     8821 actttttata ttttatgcca tcgattttgg tggtaccgtg aaaacctggt ttgccgttgg
     8881 cttgttgttt atggtgtggt ttaacgactc atttgcctat tttgccggtc gtttttgggg
     8941 taaaaggaag ttatttgagc gtatttcccc aaaaaaaact tgggaaggct tttggggagg
     9001 gctggtaatg accatagttt gcggggttat tatgagcttc cttttcgacg aattaggcct
     9061 ggttcaatgg atggtttggg ggctaattgt atcggtgagc ggcaccctgg gtgatttggc
     9121 tgaatcgcta ctgaaacgga gcattcagat taaagacagc ggctctgttt tacctggtca
     9181 cggtgggttt ttggaccgtt ttgacggctt cattatggtg atacccttta cggtagccta
     9241 cttagaaata ttgaggttct ttcacttagt ttaaccaaaa cgaacaattt tgacgcacaa
     9301 attgtattac ctttgcatcc ctgaatcatt aaggggacat aactatgctt agactcattc
     9361 ataaagaagg tcgcaaaatt attatcgctt ttttggcttt tttagtagcc ttaaactttg
     9421 ccacacatta cttcttgccg gaacaaggtt tatttaatac cttgatttta gtagcctctt
     9481 tgggtggaat tttgtttgtg acgatgtttt tccgcaaccc gaaacgccgc ttgtttactg
     9541 aagaaggagc cataatagct ccggcagacg gcaaagtggt ggtaatagaa gaaactaccg
     9601 aaaacgaata ctttaaagac cgcagattgc aggtttccat atttatgagc gtgaaaaacg
     9661 tgcatattaa ccgcacgcct atcagcggcc aggtgaaata cttcaaatac catcccggta
     9721 aatatctgtt agcttgggac cctaaatctt ctaccgaaaa tgaacgtact acctacgtaa
     9781 ttgaagatga agatgacaat atggagattt taatgcgcca aattgctggg actgttgccc
     9841 gccgaatcaa gttttatgtg gaggaaggcg atgaagttac gcaaggtgct gaattcggct
     9901 ttatcaaatt tggttcacga gtagacttgt ttttaccact tgactcaaaa atcgaggtga
     9961 atattggcga aaaggtaaaa ggtggtcaaa cggtaattgc ccgtgttgcc agaacaaaat
    10021 aaattgatta attttatcct acaataataa aacaactact atgaaaaaaa tattcttatt
    10081 cgcagcagta atggcattcg gtattgctgt ttcttttggt caaacagcag tgtcatcttc
    10141 aactacagaa tctgcatcac cggttattaa agtcactgat gctactggac ctgcgaaatg
//
LOCUS       DAORKW010000066        10180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_143_length_10180_cov_5.428938, whole genome shotgun sequence.
ACCESSION   DAORKW010000066 DAORKW010000000
VERSION     DAORKW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143_length_10180_cov_5.428938"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="PLB46_04395"
     CDS             <1..1587
                     /locus_tag="PLB46_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75787.1"
                     /translation="NGGGDCGESNNSIILGKRKNATIDNSGCKTDEFALTLAAADFNA
                     AGDTIWFKMVNTGGGGLGDMTDHYVYGLWNGALGADVQSQIKFQTATDFTNLPSGQGA
                     MNYLNFKPNGNPTQLAGDQDAFYFYYDIDQTVGSTKDVLYDYTKYLWTDESIRGYRLD
                     AVKHFPGWFVGDLLDDLHDNGMNPGMVVGENYDFTPSVLKGWLDDVMFNMDGDTKDAI
                     NVRVFDFALRNNLEQACDAFGYDVRNVFNSGIVDGAGGSGFNVVTFINNHDFRDAGQP
                     VDNDPMLAYAYILTNNKLGIPCVFFKDYYPLNLRYQIDGLMEVHKRYIYGATQVDYLS
                     RFASPYTANYIAGGANTSLIYQTSYAESGREVVVVINFSGSTLKVDQTINTANISPGD
                     TLTDIFSQTAFPFITVQGDNQIYLEIPARSYAVYVEGDLSDELIDISTPVNIAQLANA
                     VTFDVFPNPVDDVLYFHISDNNSSNQQVNIIDMTGKLIYSTTLSPTENTISTKNLISG
                     TYLIQIIRDEEIRIAKFIKQ"
     gene            1589..3223
                     /locus_tag="PLB46_04400"
     CDS             1589..3223
                     /locus_tag="PLB46_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HRF75788.1"
                     /translation="MSTEQSASIFAGIFPSADQIPENAKLDLPIKQSQYLINGEFRKW
                     NGAFTKIYSPVYLKNGDNFEQVLVGEIPHYTIEEAMSALHAAVKAYDLGRGEWPTMPV
                     KKRIGCLINFTEKMKTKREEVVRLLMWEIGKSRADSEKEFDRTVQYIYDTIDALKELD
                     RTTSKIQKEEGIYAQIRRGPLGVVLCMGPYNYPLNETFSTLIPALIMGNSILFKPAKY
                     GVLLINPLQECFRDSFPAGVVNMLYGDGKEIITPIMESGKVDVFAFIGTSRVANIIRK
                     SHPKPNRLRACLGLEAKNPAIILPDADLEIAASECITGSLSFNGQRCTALKMIFVHES
                     IAVPFMDLYKQKLAALKYGMPWSEKVMLTPLPEDNKPAYLKGLIDDAKSYGAQIINEH
                     GGDNFLSFNYPAVLFPVNEKMRVWHEEQFGPVIPIATFSDINEPITYIQNSNYGQQVA
                     VFGTDHDKMAKLIDPLVNQVCRVNINSQCQRGPDKYPFNGRKDSAEGTLSVQDALRVF
                     SIRTTVAFKDSAINRDMVNDILENRKSNFLSTDYIL"
     gene            3252..4733
                     /gene="malQ"
                     /locus_tag="PLB46_04405"
     CDS             3252..4733
                     /gene="malQ"
                     /locus_tag="PLB46_04405"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRF75789.1"
                     /translation="MKKRASGILLHPTSLPGKYGMGALGNEAKAFIDFLHLAKQTYWQ
                     ILPLGPTGYGDSPYQCFSSNAGNPYLIDIDTLVNDGLITTADIPELTVSDDTRIDYGS
                     VIDHKMGILQTAKNNFKPEGNNEYTFFLQANAHWLNDYALFMALKEKFDKRPWYEWEN
                     DYRLRNQSVLLKETLALKDSIEFQQFIQYVFFKQWKDIKNYANSKGVQIIGDIPIYVA
                     MDSSDTWCNPELFQFDNNKNPIVVGGCPPDYFSETGQLWGNPIFNYHKMEEDGFAWWI
                     NRIRASLALYDYVRVDHFRGFAGYWSIPYGEPTAVNGKWVDGPGKKLFYAIKNVLGDI
                     PIIAEDLGLITPDVTELRDHFNLPGMKILQFAFDSAESNDYIPHNYIKNCVVYTGTHD
                     NDTVLGWYEKAKEADQKYFLEYIHSDGSDICKDLIRVAWASVANTAIIPMQDLLRLGG
                     DARMNLPGTTVNNWLWRMKADDLRTELAEELAALTKLYDRISR"
     gene            complement(4737..5381)
                     /locus_tag="PLB46_04410"
     CDS             complement(4737..5381)
                     /locus_tag="PLB46_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75790.1"
                     /translation="MKKYALLLVVVIFTITAKGQDTDKAPYAIKYDFFTPVAGCFGFS
                     LEIPRTNFTSLDIDAGLIGLKLGDYYYNETFGGGYASIGPRYYFRKDELPKNDMRGPY
                     FKPQVLLNYFQYTYDYGYYDPVSNQSIISEVNGSDFSANILLCMGSQWILSDLIVFDL
                     WFGLGYGGDWTKEETNVPPDYFYEYNSRSYKYSHVHFGDSPMIFDGGLSIGLVF"
     gene            complement(5428..6714)
                     /locus_tag="PLB46_04415"
     CDS             complement(5428..6714)
                     /locus_tag="PLB46_04415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF75791.1"
                     /translation="MLAIQKRLSNSFYAILAMPATAMGFALCVQISALSWILSTKYNL
                     KIEEIGLVWASGPLAGLVMQLLVGLVSDNAWFWGGRRRPFIIIGGTIAALMLICLPNL
                     DVIQKLFGAESLIPVALAVATTLDLAINLGFNPTRSIITDVTPDGKARTKGYTWMQTV
                     SGTLGVGAYFLGAYFSNYTLIYVGVFIVLAFSIIPVFFIEEPKQLQADTTDAEINALD
                     VQAHTQKAEINELIKIYFANAFSWIGVQTMFVFMFAYASQMLFNTNDSASLDETQNNK
                     IGQIIGYSFLILNAVGAFLPAIVLEPLTKKFGRIAVQSTCLLIMSIAYGLIVWIGNSV
                     TALYCCMALAGIGWAAIVSLPFAIMSEKVNKQRMGLFMGIFNMSIVIPQLIVSLLIAQ
                     FIGSAENKDLIFEISAVSLFLSFIMWRLVKEKRATA"
     gene            6928..8277
                     /locus_tag="PLB46_04420"
     CDS             6928..8277
                     /locus_tag="PLB46_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF75792.1"
                     /translation="MEKPRMSFWQIWNMSFGFLGIQFGFALQNGNASSILQNFGADIH
                     HLSWYWLVAPITGMIVQPIIGHYSDNTWTKLGRRKPYFLLGTILCCLALVLLPNSAFF
                     LAGKSALLIGAGFLMIMDASINIAMEPFRALVADNLPDKQRTLGFSIQTVLIGVGAVA
                     GSWLPWVLANWANVSASAPQGQVANNVIYSFYIGAIVFFGAIMVTIIFAKEYPPKEYE
                     QYHGKPQKSNKGIWEIFSDFSKMPKTMKQLGLVQFFSWFALFGMWVFTTPAIAHHIYK
                     LPVDDTSSDEFRKAGNWVGIIFGVYNLVSAIYAIFLPAIARKIGRKFTHAISLTCGGA
                     GLISMFFANSPEMLIFSMIGVGIAWASILAMPYAILAGSIPAGKMGVYMGIFNFFITI
                     PQIINGIVGGPIVHAIYNDNAIYAIVMSGVFMICAAVSVIYVHDDGDITAIKTKPIA"
     gene            complement(8278..9369)
                     /locus_tag="PLB46_04425"
     CDS             complement(8278..9369)
                     /locus_tag="PLB46_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRF75793.1"
                     /translation="MTQDQSIPIRKRAVHFDLVRFIATFMVVQLHVATPWLWRELDVN
                     SGFWITCLIFDTFGRVGVPIFLMISGALLLSKEESYPSFFKKRFVRVLLPLIFWTIVY
                     EIKIIADHHYLKGGVEWSTLYSVMKNPFTGPVYDHLWFLYMIVGMYLVTPFLRRIIPV
                     LRKQDLYYLLGLWVFSNVILPLVYNYTTFKLGLSIPVVTSYLGFYVLGYYLMQYQFNA
                     KQIKIAWITFAVSYIINILLVWMDSNQHDKVDIFYLSHHRPGIFVQAVAAFILLEHYA
                     MKAFANFSEKKYKLLAYLGSVTFGIYLLHPLIIELFEGVFMGIIGIYPPAMTTAPVIT
                     IPITAIIVTSITMGIVTLMQKTPFLKNFV"
     gene            complement(9563..>10180)
                     /locus_tag="PLB46_04430"
     CDS             complement(9563..>10180)
                     /locus_tag="PLB46_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75794.1"
                     /translation="PEAKGDTSATFDASLQGPLAVPGKYMVKMYKGNTLIGEEQFEIK
                     KDPRNPSTQQELQAQFDLNVKICNKLNEISAATATIRSLRKQINEHSAGIKDPAQKEK
                     FEAIATPVLDSLTAIEEGLVNPKILAYEDNLKFPIMLEEKLAGLNYFLQMADTGPTKS
                     MIAKYDDLNKRIEVFLSAYRKMVPSEINKINAAVGTQNSGLLKIE"
BASE COUNT         3270 a         1829 c         1933 g         3148 t
ORIGIN      
        1 aatggcggtg gagattgtgg tgaaagtaat aacagcatta ttttaggtaa aagaaaaaat
       61 gccacaattg ataatagcgg ttgtaaaacc gacgaatttg cacttacact tgctgccgca
      121 gattttaatg ctgccggaga taccatttgg tttaaaatgg taaacaccgg aggaggtggt
      181 ctcggtgata tgaccgatca ttatgtatat ggattgtgga atggtgcttt aggggcggat
      241 gttcaatcgc aaataaaatt tcaaactgct accgatttta ccaatttacc aagcggtcag
      301 ggagcaatga attatcttaa tttcaaaccc aatggtaatc ctacacaatt agccggcgat
      361 caggatgcat tttattttta ttatgatatc gatcaaactg taggttcaac taaagatgtt
      421 ttatacgact acacaaaata tttatggact gatgaaagta tacgcggtta tcgtttagac
      481 gctgttaaac attttcccgg atggtttgtc ggcgatttat tggatgattt acatgacaat
      541 ggcatgaacc cgggaatggt tgttggtgaa aattatgatt ttacaccttc agttttaaaa
      601 ggttggttgg atgatgtcat gtttaatatg gatggcgaca caaaagatgc aatcaatgtg
      661 cgtgtatttg attttgcttt gagaaataat ttagaacaag cttgtgatgc atttggctac
      721 gatgtgcgta atgtatttaa ttcaggtata gttgatggtg ccggcggtag cggatttaat
      781 gttgtcacat ttattaataa tcacgatttc agagatgccg ggcagccggt tgataatgat
      841 ccaatgttag cttatgccta tattctcact aataataagc tgggcattcc ttgcgtgttt
      901 tttaaggatt attatccttt aaatctgcgt taccaaattg atggtttaat ggaagttcat
      961 aaacgatata tttatggtgc aactcaagta gattatttaa gccgatttgc ttcaccatat
     1021 accgcgaatt atattgcagg tggtgcaaat acctcgttaa tttatcagac aagttatgca
     1081 gaaagcggac gggaagtagt tgttgttatt aatttttccg gaagtacatt aaaagtagac
     1141 caaacaatta atacggcaaa catatctccc ggtgatactt taacagatat tttttcgcaa
     1201 actgcttttc catttattac tgttcagggc gacaatcaaa tttatctcga aattccggcg
     1261 cgcagctatg ctgtgtacgt tgaaggagat ttaagtgatg aactgataga tatttcaact
     1321 ccggtaaata ttgcgcaatt agcaaatgca gttactttcg atgtatttcc aaatccggtt
     1381 gatgacgtat tatattttca tatcagcgat aataattctt ctaatcaaca ggtgaatatt
     1441 attgacatga cgggtaaatt aatttattcc acaactttat ctcctactga aaacacgata
     1501 tcaacaaaaa atttaatttc aggcacttat ttaattcaaa ttatccgtga tgaagaaatc
     1561 cggatagcaa aattcattaa acaataacat gagtacagaa caatcagcat ccatttttgc
     1621 aggaatattt ccttcagcag accaaattcc cgaaaatgca aaattggatt tacccataaa
     1681 acaatcgcaa tatttaatca atggtgaatt cagaaaatgg aacggagcat ttacaaaaat
     1741 ttattcccct gtatatctga aaaatggtga taattttgaa caggtgcttg ttggtgaaat
     1801 tccacattac actattgaag aggcaatgag cgccctgcac gctgccgtta aggcttatga
     1861 tttaggtcgc ggcgaatggc ctaccatgcc tgtgaaaaaa cgcattggct gtttaattaa
     1921 tttcaccgaa aagatgaaaa caaaacgcga agaagtggtg cgtttgttaa tgtgggaaat
     1981 tggaaaatcg cgcgccgatt ctgaaaaaga atttgacaga acagtccaat atatttatga
     2041 tacaatagat gccttaaaag aattagaccg caccacttct aaaattcaaa aagaagaagg
     2101 tatttatgca caaattcgca gaggcccgct tggtgttgtg ttatgtatgg ggccatacaa
     2161 ctatccttta aatgaaacat ttagcacttt aattcccgca ttaattatgg ggaatagcat
     2221 tttatttaaa ccagcaaaat atggtgtatt attaattaat cctttgcagg aatgtttccg
     2281 cgatagtttt cctgccggtg tggttaatat gttatatggt gatggtaaag aaattattac
     2341 accaataatg gaatcgggta aagtagatgt atttgcattt attggcacaa gccgagttgc
     2401 caatataatt cgcaaatcgc atcctaaacc aaacagacta cgcgcgtgtt tagggcttga
     2461 agcgaaaaat cctgcaatta ttttacccga tgccgatttg gaaatagctg ccagtgagtg
     2521 tattaccgga agtttatcgt ttaacggtca gcgctgtacg gcattaaaaa tgatttttgt
     2581 gcatgaaagt attgccgttc cgtttatgga tttatacaaa caaaaattag cagctttaaa
     2641 atacggtatg ccatggagcg aaaaagtaat gttaactcca ctaccggaag ataataaacc
     2701 ggcttattta aaaggattaa ttgatgatgc aaaatcgtac ggtgcacaaa taattaacga
     2761 acatggtggc gataattttt taagttttaa ttatcctgca gtactatttc ccgtaaatga
     2821 aaaaatgcgt gtttggcatg aagaacaatt tgggccggta attcctattg ccacatttag
     2881 cgacattaat gagccaatta cctatatcca aaacagcaac tatggtcagc aggtggcagt
     2941 gtttggcacc gaccacgata aaatggctaa actaattgac ccgcttgtaa atcaggtttg
     3001 tcgcgtaaat attaactctc aatgtcagcg cggccccgat aaatatccgt ttaacggaag
     3061 aaaagattcg gcagaaggaa cactgagtgt gcaggatgct ttacgtgttt tcagtatacg
     3121 gacaacggtt gcgttcaaag attctgcaat caaccgcgat atggtaaacg acattttaga
     3181 aaaccggaaa tcgaatttcc tttcaacaga ttatatattg taaaatcctt tccgatatat
     3241 ttgctattgt tatgaaaaaa cgcgcatcgg gaatattatt acatccaaca tcgttacccg
     3301 gcaaatatgg catgggtgct ttgggtaatg aggctaaagc ttttatcgat tttttacatc
     3361 tggccaaaca aacctattgg caaatattac cacttgggcc aaccggttat ggcgattcac
     3421 cttatcaatg tttttcgagc aatgccggca atccgtattt gatagatatt gatacattgg
     3481 taaatgatgg tttaataaca acagctgaca ttcctgagtt gactgtaagc gatgatacca
     3541 gaattgatta tggctcggtg atagaccata aaatgggcat tcttcaaact gctaaaaata
     3601 attttaaacc ggaaggcaat aacgagtata ctttttttct tcaagctaat gcgcattggt
     3661 taaatgatta cgccttgttt atggcgttaa aagaaaaatt tgataaacgt ccttggtacg
     3721 aatgggaaaa tgattatcgc ctgcgcaatc aaagtgtgtt attaaaagaa acacttgcat
     3781 taaaagacag catcgaattt caacaattta ttcaatatgt attttttaag caatggaagg
     3841 atattaaaaa ttatgctaac agcaagggcg ttcaaataat tggcgacatt cccatttatg
     3901 tggcgatgga tagcagcgat acctggtgta atccggaatt atttcaattc gacaataata
     3961 aaaatccgat tgtggttggt ggatgtcctc ctgattattt cagcgaaacc ggacaattat
     4021 ggggcaatcc tattttcaat tatcataaaa tggaagaaga tggatttgcg tggtggataa
     4081 atcgtattcg cgcaagttta gctttatatg attatgtgag ggttgatcac ttcagaggat
     4141 ttgccggata ctggagtatt ccatacggag agccgacagc agtgaacggc aaatgggtag
     4201 atggtccggg taaaaaatta ttttatgcca ttaaaaatgt tttaggcgat attccaatca
     4261 ttgccgaaga tctcggttta attacacctg atgttaccga attgcgcgac cattttaatt
     4321 taccgggaat gaagattttg caatttgcat ttgatagtgc cgaatccaac gattacattc
     4381 cacataatta tattaaaaat tgtgttgttt acaccggcac tcacgacaac gatactgttt
     4441 tgggctggta tgaaaaagca aaagaggcag atcaaaaata tttcctcgaa tatattcaca
     4501 gcgatggcag cgatatttgt aaagatttaa ttcgtgttgc ctgggcaagt gtagcaaata
     4561 ctgctataat tcccatgcag gatctattgc gtcttggagg agatgccaga atgaatttgc
     4621 cgggaacaac cgtaaataac tggttgtggc gaatgaaagc agatgattta aggacggaat
     4681 tggctgaaga gttagcagcc cttaccaaac tttatgatcg catttccaga taaatattaa
     4741 aacaccaacc caatacttaa cccaccatca aatatcatag gtgagtcgcc aaagtgtacg
     4801 tgactatatt tatatgacct gctgttatac tcataaaagt aatctggtgg aacattagtt
     4861 tcttcttttg tccaatcgcc gccataacct aaaccaaacc acaaatcgaa tactattaaa
     4921 tcagataaaa tccattgact tcccatgcac aataaaatat ttgcagaaaa atcggagcca
     4981 tttacttcac tgataatgct ttgatttgaa accgggtcat agtatccata atcgtaagtg
     5041 tattgaaaat aattaagtaa aacctgaggt ttaaaatacg gaccacgcat atcgtttttg
     5101 ggtaactcat cctttctgaa ataataccta gggccaatag atgcgtaacc acctccaaat
     5161 gtttcgttgt aataataatc gcctaatttc agcccaatta atccggcatc aatatcaagc
     5221 gaagtaaaat tagtacgagg tatttctaat gaaaatccaa aacaacctgc aaccggtgta
     5281 aaaaaatcat attttattgc ataaggagct ttatctgtat cctgaccttt agctgttatt
     5341 gtgaaaatta caactacgag taatagcgca tattttttca tagtgatgaa ttttatgacc
     5401 taaagttacc ttaaaaatat cgaataatta cgctgttgca cgtttttctt tcaccaatcg
     5461 ccacataata aaactcaaaa aaagacttac tgcgcttatc tcaaatataa ggtccttgtt
     5521 ttctgcgcta cctataaatt gtgcaattaa taaacttaca atgagttgcg gaattactat
     5581 actcatattg aatattccca taaacaaacc cattcgctgt ttattcactt tttcgctcat
     5641 aatagcaaaa ggcaagctta caattgccgc ccagcctatg ccggccaaag ccatgcagca
     5701 gtataacgcg gtaacactat tgccaatcca aactattagg ccgtaggcaa tactcataat
     5761 taataagcag gtggactgaa ctgctatacg gccaaatttt ttcgttaagg gttccaaaac
     5821 tatggctggc aaaaacgcac ctaccgcatt taatatcaaa aacgaataac cgataatttg
     5881 accaatttta ttgttttgtg tttcatcgag ggatgccgag tcgtttgtat taaataacat
     5941 ttgtgaagca taggcaaaca taaaaacgaa cattgtttgc acgccaatcc acgaaaacgc
     6001 attagcgaaa taaattttta tcaactcatt aatttcagct ttttgtgtgt gagcctgcac
     6061 gtcgagcgca ttaatttctg catcggttgt gtcggcctgc aattgttttg gttcttcaat
     6121 aaaaaacaca ggaataatac taaatgccaa tacgataaaa accccgacat aaattaacgt
     6181 atagttacta aaatatgctc ctaaaaaata tgcaccaact cctagtgttc ctgaaactgt
     6241 ttgcatccag gtataccctt ttgttcttgc tttcccatcg ggagtaacat cggtaataat
     6301 acttcttgtt gggttaaaac caaggtttat agcaaggtct aatgtagtag ctacagccag
     6361 tgctaccgga ataaggcttt ctgcaccaaa caatttctga ataacatcta agttgggcaa
     6421 acaaatcagc attagtgccg caatagtgcc gccaataata ataaatggcc ttcttcttcc
     6481 accccaaaac catgcattgt ctgatactag tcccaccagt aattgcatca ccaaacctgc
     6541 caaaggaccg cttgcccaaa ccaatccaat ttcttctatt ttgagattgt atttagtaga
     6601 taaaatccag ctcagggcgc ttatttgcac acatagtgca aaacccattg ctgttgcagg
     6661 cattgccagt atggcataaa atgaattgct gagtcgtttt tgaatagcta acatgttgaa
     6721 gaattaggtg aacactgttc ggttgaataa gcaataaata tacaaaaacc cttaacgaag
     6781 gcaagttgta aaataagaaa acaagtgtat cagacattgc atattgtaaa ttttacaaaa
     6841 agtgttatct gcgcatttta tcgggtggca taaattcaga tatatcccta tcattcctta
     6901 aattgcgacc aaattaaaca ggacaacatg gaaaagcctc gcatgagttt ttggcaaatc
     6961 tggaatatga gtttcggatt tttaggaata cagtttgggt ttgccttgca gaatggtaat
     7021 gcatcatcaa tattgcaaaa ctttggtgcc gatattcacc atttgagttg gtattggtta
     7081 gtagctccaa ttacaggtat gattgtccaa ccaattattg gacattatag cgataacacc
     7141 tggacaaaac ttggccgcag gaaaccttac tttttgttag gtacaatttt atgttgttta
     7201 gcgttggtgc tgttacctaa ttcagcattt ttccttgccg gtaaatccgc tttgttaata
     7261 ggtgccggat ttttaatgat tatggatgcc agcattaata ttgccatgga accattcaga
     7321 gcgctggttg ccgataactt acctgataaa caacggacac taggtttcag tatacaaact
     7381 gtactcattg gtgttggagc ggttgcaggc agctggttgc catgggtttt agctaactgg
     7441 gcaaatgttt ctgcttcggc gccacaaggt caggttgcta ataatgttat ttattcattt
     7501 tatatcggtg caattgtatt ttttggtgct attatggtga ccatcatatt tgcaaaagaa
     7561 tatccgccaa aagaatatga acaatatcat ggcaaaccac aaaaatcgaa taaaggcatt
     7621 tgggaaatat tttccgattt cagtaaaatg cctaaaacaa tgaaacagtt aggtttagta
     7681 caatttttta gctggtttgc attatttggc atgtgggtgt ttacaacgcc tgcaattgca
     7741 catcatattt ataaattacc cgttgatgat acatcgagcg atgaattcag gaaagcaggt
     7801 aactgggtgg gaattatttt tggtgtgtac aatttagtga gtgcaattta tgctattttt
     7861 ttaccggcaa tagcaagaaa aatcggaagg aaatttacac atgccatttc tttaacctgt
     7921 ggcggcgccg gattaatttc tatgtttttt gctaattctc ctgaaatgtt aatcttttct
     7981 atgattggcg ttggtattgc atgggcaagt attttagcta tgccttatgc catattagcc
     8041 ggaagtattc ctgcaggaaa aatgggtgta tatatgggta tatttaattt ctttattacc
     8101 ataccacaaa taataaatgg tattgttggc ggaccaattg tacatgccat ttataacgat
     8161 aatgccattt atgcaattgt tatgagcggg gtatttatga tttgcgcagc agtgtcagtt
     8221 atttatgtgc acgacgatgg tgacatcact gcaattaaaa caaaacccat tgcttaatta
     8281 aacaaagttt ttcaggaaag gggttttttg cataagcgta acaataccca ttgtaattga
     8341 agtaactatt attgccgtaa taggtatggt aattacaggt gcggttgtca tggcaggagg
     8401 gtaaatgcct attataccca taaaaacacc ttcaaacaat tcgataatga gcggatgtaa
     8461 taaataaata ccaaatgtta cgctgcctaa atatgccaat agtttgtatt ttttttcaga
     8521 aaaatttgca aatgctttca tggcataatg ttcaagtaaa ataaatgcag caaccgcctg
     8581 aacaaatatg cccggtctgt ggtgagataa ataaaaaata tcaaccttat cgtgctgatt
     8641 ggaatccatc caaaccaata aaatatttat gatataacta acggcaaatg ttatccaggc
     8701 aattttaatc tgtttagcat taaactgata ttgcattaaa taatatccca gtacataaaa
     8761 ccctaaataa gaagtaacta ccggaatact taaaccaagt ttaaacgtgg tgtaattata
     8821 tacaagtggc agaatcacat ttgaaaaaac ccataacccc aataaataat ataaatcctg
     8881 tttccgtaaa accggaataa ttcttcgtaa aaatggtgtt accagataca tgccaacaat
     8941 catgtacaaa aaccacaagt ggtcataaac cggtcccgta aatgggtttt tcatcaccga
     9001 ataaagtgta gaccattcta cccctccttt taaataatga tggtcggcaa taattttaat
     9061 ttcgtaaaca attgtccaaa aaatcaatgg caaaagcacc cttacaaacc gttttttaaa
     9121 aaaagaagga taagactctt ctttcgaaag cagcaaggcg ccgctaatca ttaaaaaaat
     9181 gggcacccca accctgccaa acgtgtcgaa aatgaggcaa gtaatccaaa aaccgctgtt
     9241 tacatcaagc tcacgccaca gccacggtgt tgcaacgtgt aattgtacta ccataaaagt
     9301 ggcaataaat cgaaccagat cgaagtgaac ggcccgtttt cgtatcggaa tagattggtc
     9361 ttgcgtcatg ctgaaaacgt agctgccagg cagcctgttt aacacacggc aaatatacga
     9421 ttattaaata gttaacaatg tgcgatgtgt ttataacgca tacgagaggt gaagggcctt
     9481 gaatatcgcc aataaaaaag gggaaacaac acttaatttg ttgttccccc atttaatatt
     9541 atactttata agtgttaagt tattattcga ttttaagcaa gcctgaattt tgtgtgccaa
     9601 ctgctgcgtt tattttgttt atttctgacg gtaccatttt ccgataagca cttaaaaata
     9661 cttctatacg tttatttaaa tcatcgtatt ttgctatcat cgatttagtt ggaccggtgt
     9721 cagccatttg caagaaataa tttaatcccg ccaacttttc ttccaacata attggaaact
     9781 ttaaattgtc ttcataagca agaatttttg gatttaccaa cccttcttct atcgcagtaa
     9841 ggctatccaa caccggtgtt gcaattgctt cgaatttttc tttttgagcc gggtctttaa
     9901 tgcctgcact atgttcgttt atttgtttgc gtaaacttct tattgtagcc gttgctgcag
     9961 aaatttcatt taatttatta cagattttta catttaaatc gaactgcgct tgtaactctt
    10021 gttgagtaga tggattgcgc gggtcttttt taatttcaaa ttgctcttca ccaataagtg
    10081 tattgccttt atacattttt accatatatt ttccgggcac agctaagggt ccttgcaacg
    10141 atgcatcaaa cgtagcactg gtgtctcctt tagcttccgg
//
LOCUS       DAORKW010000067        10138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_146_length_10138_cov_6.730140, whole genome shotgun sequence.
ACCESSION   DAORKW010000067 DAORKW010000000
VERSION     DAORKW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146_length_10138_cov_6.730140"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PLB46_04435"
     CDS             complement(<1..803)
                     /locus_tag="PLB46_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF75795.1"
                     /translation="MENHQFRPESLMMSYGYKPELSEGAIKCPIFQTSTFVFKNAEAG
                     KRFFEVAYGLSEQRPGEEQGLIYSRLNNPDLEVLENRLTLWDGAEACAVFSSGMSAIS
                     TVLLEFLKPGDFVLFSAPVYGGTDHFIKHVLPQWGIESASFDANDSEAAIAAIIEAKG
                     QKKLGLIYVETPANPTNALIDLKMCKRVADKFSAPDKPVYLAVDNTYMGPLWSHPLKH
                     GADLVMYSATKYIGGHSDVIAGAVLGNNALMTRIKTMRTFMGNMMSPWTG"
     gene            complement(952..1593)
                     /locus_tag="PLB46_04440"
     CDS             complement(952..1593)
                     /locus_tag="PLB46_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF75796.1"
                     /translation="MNNIKVLLFDDNIRIRESLSILFNATRGFEFCGAYADNMQVVEL
                     VELHQPNVVLMDIDLPGINGIEAVRRIRKTNTDLPILMLTVFDDDEKIFGAICAGASG
                     YMLKNKSLDQLIDAVREVSLGGAPMTPSIAKRVLELFQKQNKLETADDFNLSPREKDV
                     LGHLVKGNSYKMIADELQIGYDTVHSHIKKIYKKLHVNSMTEAVAKALKNKLT"
     gene            complement(1646..3469)
                     /locus_tag="PLB46_04445"
     CDS             complement(1646..3469)
                     /locus_tag="PLB46_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF75797.1"
                     /translation="MKYLFTLASFFVLFGANLYASRTDTAYINQINKRSFELREANPD
                     SCKLLADEAIALSEKINYAKGLGDGYTRLGLLEKNKGNFEGAIRFYQKSLYYRKQLND
                     PELVARVYSNIGNVFHNSAQFDSAIFYHLESVKIAESLKDDGLISKYSGNLGIAYQKN
                     ESPEEALKYYTYSYEYAQKTGNTDAILLSTINLSSLYIDMNELRKAIGYLLQIEKTLS
                     EDAAPDDVGNIYNNLGTAYNNLNKVDSALYYFEKAAVIYAEEELPELGVCLSNMGILY
                     FKTGNPEKAKLYLHNSNVLLLEANRLEVVEDNYMYLANVFSFQGKFDSAEIMMEQHII
                     FRDSVYNQEKASKISEIQTQYETEKKEQIIKRQKLINTGIIVAAVLVLAILLLLFNRR
                     QLKKKLEFEQQLVSDRTRISSDLHDDIGSTLGSISYFSQLVTKLQKENDADKVNVLLA
                     KIEEVSGEAVENMSDIVWAIKPDNDSSEKMFLRIQNYASELQQAAELNYVSKIEESAN
                     AISLNMEQRKNIYLIFKESIYNAFKYAEATEINFNANYDGNMLTITLTDNGKGFDPEK
                     TNSINGNGLGNLHRRAKEIGATLNVKSANKQGTTITVTLPI"
     gene            complement(3505..4182)
                     /locus_tag="PLB46_04450"
     CDS             complement(3505..4182)
                     /locus_tag="PLB46_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75798.1"
                     /translation="MLQSFKPHIFIDHATATFTFLINTNSSNGNYTINSTINEFDDPY
                     QNGVRVYEFTLVTSSATVNNEPISSSLPSGSSGNYNKISVRILFPNGSAAGSVTVNIK
                     NEEHFTHDGTNVMPYCWIKSLPVPATKVFFKTFVPNTVLPSQPTEIVLLPAKNKIVLN
                     YIVPPLAANPNNLFSERSYNLSDYDPATYNLIEVRITEEDSSNNTRKKKGSGTTSQSD
                     ADSSDDN"
     gene            complement(4192..5289)
                     /locus_tag="PLB46_04455"
     CDS             complement(4192..5289)
                     /locus_tag="PLB46_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF75799.1"
                     /translation="MGSIIFKEIQDFRKQQTAFTVHPKPLTIDSLNKTDEQYVSEFIS
                     PAQYYKINNGGKHNVIALLDTGVYAEHKALQNKVIASMMYDGSTKGPNGKLTDEHGHG
                     THLAGILVADHITISYNIDGNTKTEKIGGMIPDARLVSIRVTADNSGSTSWYAIAEGL
                     EQVFNYNLKPGMKEDDKIKIVVLAYNAFDNVNTDYPVCNHRINKLIQGLYKMGIAVVV
                     SAGNAYEYYQPTFTKSIASGLAYPAYFKNTLVAMANDSDNKLSSFTQRNCDEDHDFGN
                     HVFSAVGADTISTGIGAVDSVSVLSGSSQAAAVLAGLYIQKLTHISSGTSNLHPSQIF
                     FELNQNATIQNQNMFDASRSTHVKHFKIANL"
     gene            complement(5359..6231)
                     /locus_tag="PLB46_04460"
     CDS             complement(5359..6231)
                     /locus_tag="PLB46_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75800.1"
                     /translation="MRYFFIVSIFLLLILGCGKRELPQLNDGTETVVEQSVYVVHSMI
                     YADSLPWTTSNIYIIKGTGDTVWIYGSGYGDFADMCSDCNDNNYYLGAKFYGTGPAIA
                     DVNQVDSVITKIFDLHRDSVLLQFIVPHYHNDHINNEFIDAFHNKLNYPLRSDEKIWI
                     HTNDAYGAICNEPCCGTEPCPDKKNKYYGVPFHPAWQKEYINLFTKMGETDDDCNAVI
                     KTFTSSTGEWQITKALAVENGGHTDGTVNLHNNSLQLRIAGTKSKKQCPLPEGWRMIS
                     VHGNVNGEDNSPGE"
     gene            complement(6237..6935)
                     /locus_tag="PLB46_04465"
     CDS             complement(6237..6935)
                     /locus_tag="PLB46_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75801.1"
                     /translation="MKKTSILLCALVMLMATAFKSDNNCESFFPTEVGTKWEITSYDG
                     KDKVQSRSVSELTALNNVADGVEAKINIEMFDQKDKSVNKGEVIMKCTPESFFMDMSN
                     MFPKDQFEGMDDVTIEVTDQYMEFPSNPVAGQTLPDAESSMTVTMNGMQLMSMKMKTT
                     NRKVVGYESVTTPAGTFNCIKYTYDSEFASTMFKSKSSSTMYMAKNVGMVKMESIDEK
                     GKYPSKQILTSFSK"
     gene            complement(6960..>10138)
                     /gene="mfd"
                     /locus_tag="PLB46_04470"
     CDS             complement(6960..>10138)
                     /gene="mfd"
                     /locus_tag="PLB46_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRF75802.1"
                     /translation="KDILFIPDSFKKETSFEEVNTNSVLLRTEAINKITNSHTRGEII
                     VTYPEALVEKFVVAEVLKENIILLKKGEKLDDHFIIDLLAENGFERTDFVYEPGQFSV
                     RGGIIDIFSFGNEMPYRVELYDDEVESIRVFDPTTQISEKTISQVTIIPNIQTQFGAE
                     QKSSLFHSLPKNTIIWFKDAKYTFEVINKVYEKALDWYEVTKREKLLDRDHPFNKYPP
                     EELLLSGPALLDEVPHFSAVEFGLHSNLPDTQIIQFDAAPQPLFNRNFEQLIQKLKEL
                     QSLRYHLFIFMSNARQAERFHEIFTDLNAQVKYLPIIPVINEDGAASTTIAAGFIDND
                     KKIVCFTDHQIFERHHKYTVKSGFSKTDAITLKTLMELRPGDFVTHIDHGVGVYSGLE
                     KIEIGGQIQEAVRLIYRDNDLLYVNINSLHKISKYAGKEGTQPKLNKIGSDAWEQLKR
                     KTKAKVKDIAKDLIALYAKRKASKGFAYSPDGYLQNELEASFIYEDTPDQYRATVDVK
                     RDMEKPHPMDRLVCGDVGFGKTEIAIRAAFKAVTDGKQVAVLVPTTILASQHFKTFSE
                     RLKEFPVTVDYINRFKSDKDKKTTLEKLEAGKVDILVGTHAIIGKKIKFKDLGLLIID
                     EEQKFGVTVKEKLRAFRANIDTLTLTATPIPRTLQFSMMGARDLSIINTPPPNRQPIQ
                     TELKTFDAEFIRDAIYQEIYRGGQVYFIHNRVRDIQETAALIKKVCPDLNIGVAHGQL
                     EGEELEDHMLKFIDKTYDVLVCTNIVESGLDIPNANTIIINNAHWFGLSDLHQLRGRV
                     GRSNKKAYCYLISPPLFGLPDDSRKRLRTIEQFADLGSGFQISMRDLDIRGAGNLLGA
                     EQSGFISEIGFDMYHKILDEAIRELKQTQFKALFADQIEEKQEYVRDVQIDTDLEMLI
                     PDAYITNINERMSIYTRLDNVANEEQITQFKNELTDRFGKVPRQVEELFNGIRLRWLA
                     KDMGLERIIFKSRKLRCYFVENPESVFYDSPLFHTIMQLIAQKKTTGIIKQTDKHLIL
                     IFEQVQSMSHARQLLQNMHQNLFSTAPK"
BASE COUNT         2987 a         1928 c         1760 g         3463 t
ORIGIN      
        1 cctgtccagg ggctcatcat atttcccata aaagtgcgca tggtttttat acgtgtcatt
       61 aacgcattat tccctaaaac tgcaccggca ataacatcac tgtgtccacc aatgtattta
      121 gtagccgaat acataactaa atcggcacca tgttttaacg gatggctcca aagcggaccc
      181 atataggtat tatctacagc taaataaacc ggtttgtccg gagcggaaaa tttatcagca
      241 acgcgtttgc acatttttaa gtcgataagt gcatttgttg ggttagctgg agtttctaca
      301 taaattaatc ccagtttttt ctggcctttt gcttcaataa tagctgcaat tgccgcttca
      361 ctgtcgttgg cgtcaaaact ggcgctttca attccccatt gaggcaacac gtgtttgatg
      421 aagtgatcgg tgccgccata cacaggtgca ctgaacaata cgaaatctcc cggttttaaa
      481 aactctagta aaacggtaga aatggcactc attccgctgg aaaaaaccgc acaagcttct
      541 gcgccatccc ataatgttaa acggttttcg agcacttcca aatccggatt atttaatcgc
      601 gaataaatta atccctgctc ttctccgggt ctttgttcgc tgagtccata agcaacttca
      661 aaaaatcttt ttcctgcttc tgcattttta aaaacaaaag tggacgtttg aaatatcgga
      721 cattttattg cgccttcact gagttcaggt ttgtagccgt aactcatcat gaggctttca
      781 ggcctaaatt gatgattttc catagatggg tggtttagac gaatttgttt tcgttgtcgc
      841 aaaaatagaa ttaattattt gaccctacag tgatttttat ccccaaaaaa aatcccggca
      901 gcaaattttg acaaccggga aataaaacga gaaataattt ggatagaaaa attatgttaa
      961 tttatttttt aatgcttttg caacagcctc cgtcatggaa ttaacgtgta gtttcttata
     1021 tatttttttt atatgtgaat gtacggtatc gtaaccaatt tgtaattcat cggcaatcat
     1081 tttgtaacta ttgcctttta ctaaatgacc aagcacatct ttttcacgcg gacttaaatt
     1141 aaaatcgtcg gcagtttcta atttattttg tttttggaat aattctagaa cacgttttgc
     1201 aatggaggga gtcataggtg cacctccaag tgaaacttcg cgaacagcat caatgagttg
     1261 gtcgagcgat ttgtttttta acatgtatcc cgaagcgccg gcacaaatgg cgccaaatat
     1321 tttctcatcg tcatcaaaaa cagttaacat taaaatcggc aaatcagtgt ttgttttgcg
     1381 aattctccga acagcttcaa taccattaat accgggtaag tcgatatcca ttaatactac
     1441 attcggttgg tgcaattcta ccaattctac aacttgcata ttatcggcat acgcaccaca
     1501 aaattcaaat ccccgggtag cattaaacaa tattgaaagc gattccctta tgcggatatt
     1561 gtcatcgaat aataaaactt taatattgtt cattgttgca aatttgagta ttcatgccgg
     1621 gaaattgtat ccccaaacat ggtgattata taggtaatgt tactgtaatg gttgttccct
     1681 gcttattggc actttttaca tttaatgttg cacctatttc tttagcacga cgatgtaaat
     1741 tgcctaatcc gtttccgtta atgctatttg ttttttcagg gtcaaatcct ttgccattgt
     1801 cggttaaagt aatcgtcaac atgttaccat cgtaattagc attaaaattt atttcagtcg
     1861 cttcagcata tttaaaggca ttataaatac tttctttaaa tattagataa atgttttttc
     1921 gttgctccat atttaaactg atagcatttg cagattcttc gattttagaa acataattta
     1981 attccgcagc ttgttgtaat tcactggcat aattctgaat gcgcaaaaac attttttcgg
     2041 aactgtcgtt atccggttta atcgcccaaa caatatcact catgttttct acagcttcgc
     2101 cactaacttc ttctatttta gcaagcaaaa catttacttt gtctgcatcg ttttcttttt
     2161 ggagtttggt taccagttga ctgaaataag aaatactgcc aagtgtggaa ccaatatcat
     2221 cgtgaaggtc tgaactaatt cttgtcctgt ccgataccag ttgttgctca aactccagtt
     2281 tcttcttgag ttgtcgtcgg ttgaataata ataataaaat agccagaacc aacactgctg
     2341 ctacaattat acccgtatta atgagctttt gacgtttaat aatttgttcc ttcttttcgg
     2401 tttcatactg ggtttgtatc tcacttattt tactcgcttt ttcctgattg taaacagaat
     2461 cgcgaaatat aatatgttgc tccatcatta tttcagccga gtcgaattta ccttgaaagc
     2521 taaatacatt tgccaaatac atataattat cttccactac ttccaggcga ttagcttcca
     2581 ataataaaac attgctgttg tgtaaataca atttagcttt ttcaggattc ccggttttaa
     2641 aatataaaat tcccatatta ctcaaacaga cacctaattc gggtagttct tcttctgcat
     2701 aaatgactgc ggctttttca aaataataca aggcgctgtc taccttattt aaattattgt
     2761 atgccgtgcc taaattgtta taaatatttc caacatcatc gggagctgca tcttcggata
     2821 aagttttttc tatttgtaat aaataaccaa ttgccttacg caattcgttc atgtcgatat
     2881 acaatgaact taaattgatg gttgataata aaatcgcatc ggtattacct gttttttgcg
     2941 catattcata gctgtaggta taatatttta aagcctcctc cggactttcg tttttctggt
     3001 aggctatgcc taaattgccg gaatattttg aaatcaatcc atcatctttt aaactctcgg
     3061 ctattttaac cgattctaaa tgataaaaaa tggcagagtc aaattgtgct gaattgtgaa
     3121 atacgtttcc aatattagaa tatactcgag caaccaactc cgggtcgttg agttgtttgc
     3181 ggtaatacaa acttttttga taaaaccgga tggctccttc aaaattacct ttgttttttt
     3241 ctagcaaacc taaacgcgta tatccatcgc ctaagccttt tgcataattt attttttctg
     3301 aaagagcaat agcctcatct gccaataatt tacacgaatc agggttagct tccctaagtt
     3361 caaagctgcg tttatttatt tggttgatat aagcggtatc cgtacgcgac gcatagaggt
     3421 ttgcgccaaa aagcacaaaa aaagaagcca acgtaaatag atatttcatt tgcattggct
     3481 tcaaatatag cagctaatat ttaattaatt atcatctgat gaatcggcat cggattgaga
     3541 ggtggtaccg gaaccttttt tcttgcgggt attgttgctg ctgtcttctt cagtgatgcg
     3601 cacttctatt aaattatagg ttgcgggatc ataatcactt aaattatagg agcgttccga
     3661 aaataaattg ttcggattag cagctaatgg aggaacaata tagtttaaca caattttgtt
     3721 ttttgccggt aataatacaa tttcagttgg ttgtgaaggc aaaacagtat tagggacaaa
     3781 tgttttaaaa aatactttag ttgcaggcac tggcaatgat tttatccagc aataaggcat
     3841 tacattggtg ccatcatgtg taaaatgttc ttcattttta atgtttacgg taacagaacc
     3901 ggcagcagaa ccattaggaa acaaaatacg caccgaaatt ttattgtaat tacctgatga
     3961 acctgatggt aatgatgaac ttataggttc attatttacc gttgcacttg atgtgacaag
     4021 cgtaaactca tacaccctta caccattttg ataggggtcg tcgaattcat ttattgttga
     4081 gttaatcgta taatttccgt tactgctgtt ggtattaatt aaaaatgtaa acgtagcagt
     4141 agcatgatct ataaatatat gtggtttgaa agattgtaac atagaataaa tttagaggtt
     4201 tgcaattttg aaatgtttaa catgtgttga gcggctggca tcaaacatgt tttgattttg
     4261 aattgtggcg ttttggttta attcaaaaaa tatttgactg ggatgtaaat tgcttgtccc
     4321 tgagctaatg tgggttaatt tctgaatata taagccggct aacacagcgg cggcctgact
     4381 gctgccactc agcacactaa ctgaatctac agctccaatt ccggtagaaa tggtatcggc
     4441 gccaacagca ctaaatacgt gattgccaaa atcatggtcc tcatcacaat tacgttgagt
     4501 aaatgatgat aatttattat cactatcgtt ggccatagca acgagtgtgt ttttaaaata
     4561 agcaggataa gctaaacccg aagcaatact ttttgtaaat gtcggttgat aatattcgta
     4621 tgcattacct gctgaaacta ccacggcaat tcccatttta tataacccct gaatgagttt
     4681 atttattcga tggttacaaa caggataatc tgtatttaca ttatcaaatg cattataagc
     4741 cagtacaaca attttaattt tatcgtcttc tttcattccc ggctttaaat tataattaaa
     4801 cacttgctcc aaaccttcag caatggcata ccatgaagtg ctaccactat tgtcagcagt
     4861 aacccgaata cttactaacc gagcatccgg tatcatgcca ccaatttttt ctgtttttgt
     4921 attaccatca atgttatagc tgattgtaat gtggtcagca accaaaatgc cggccaggtg
     4981 tgtgccatgt ccatgttcgt cagttagctt accattaggt cctttagttg aaccgtcata
     5041 catcatagag gcaattactt tgttttgtaa agctttgtgt tcagcataaa ctcccgtatc
     5101 taataatgca attacattgt gtttgccgcc gttattaatt ttgtagtatt gggcagggct
     5161 tataaactca cttacatatt gttcgtctgt tttattaagt gaatcaatag ttaatggttt
     5221 aggatgaacg gtgaatgccg tttgttgttt tctaaaatcc tggatttctt taaaaatgat
     5281 acttcccata agtgtgtttt tgttgacaca aatgtagaag tataaaacaa tactgctgta
     5341 cccaaatatg gggatagctt actcaccggg agaattatct tctccattta cattgccatg
     5401 caccgatatc attcgccagc cttcaggaag cggacattgt tttttgcttt tagtgcctgc
     5461 tatacgcagt tggagcgagt tgttgtgcag atttaccgtg ccgtcggtat ggcctccgtt
     5521 ttctacagca agtgctttag taatttgcca ttcacctgtt gaagaagtaa atgttttgat
     5581 gacagcatta caatcatcat cggtttcacc cattttggtg aataaattta tgtattcttt
     5641 ttgccatgca ggatgaaatg gcacaccgta atatttattt tttttatccg gacaaggttc
     5701 agtaccacaa caaggttcat tacatatagc accataagca tcgttggtat gaatccagat
     5761 tttttcgtcg ctgcgcagtg ggtaatttaa tttattatga aaagcatcaa taaattcatt
     5821 attaatatgg tcattgtgat aatgcggaac aataaattgt aataaaacac tatcgcgatg
     5881 taaatcaaat attttcgtaa taacactatc cacttgattt acatcggcaa tagcaggtcc
     5941 ggttccataa aattttgcac ctaaataata attattatca ttacaatcac tacacatatc
     6001 ggcgaagtcg ccatagcctg aaccatagat ccatactgtg tctccggtgc ctttgatgat
     6061 ataaatattt gaagtagtcc agggtaaact gtcggcataa atcatactat ggaccacata
     6121 aacagattgt tcaacaactg tttcggtgcc atcgtttaat tgtggcaatt cgcgtttgcc
     6181 acatcccaat atcaacaata aaaaaatact aacaataaaa aaatatcgca caaatattat
     6241 ttgctgaatg aagtaagtat ttgttttgac gggtattttc ctttttcgtc gatgctttcc
     6301 attttaacca ttcccacgtt tttagccata tacatggttg aacttgattt tgatttaaac
     6361 atggttgatg caaattcaga atcgtaagta tattttatac aattaaatgt accggcaggt
     6421 gtagttactg attcataacc aactacttta cgattggtag ttttcatttt catgctcatt
     6481 aattgcatgc cgttcatagt tacagtcata ctgctttcag catcaggtaa tgtttgaccc
     6541 gctaccggat tggaaggaaa ttccatgtat tggtcagtaa cttcgatagt aacatcatcc
     6601 attccttcaa attgatcttt tggaaacata ttactcatgt ccatgaaaaa gctttccggc
     6661 gtacatttca taattacttc acctttattc accgatttat ctttttggtc aaacatttca
     6721 atgtttattt tcgcttcaac accatcggct acattattaa gtgcagttaa ttcacttaca
     6781 ctgcgtgatt gtactttatc ttttccatcg taactggtga tttcccattt agtaccaact
     6841 tccgttggga aaaatgattc acaattatta tcgcttttaa atgcggttgc catgagcata
     6901 actaatgcac ataacaatat gcttgttttt ttcatagtag tggttttgta acaaagttac
     6961 tattttgggg cagtactaaa aaggttttga tgcatgtttt gcagtaattg ccttgcgtgg
     7021 ctcatgcttt gtacctgctc aaaaatgagg atgaggtgtt tatcggtttg tttgataatt
     7081 ccggtagttt ttttctgtgc aatcaattgc atgatagtgt gaaacagcgg actatcataa
     7141 aacaccgatt cgggattttc gacaaaatag caacgtaact tcctgctttt gaatataata
     7201 cgctctaaac ccatatcctt tgccagccag cgtaatcgga tgccgttaaa caactcttcc
     7261 acctgtcgcg gaactttgcc aaaacggtcg gtgagctcgt ttttaaactg tgtaatctgt
     7321 tcttcgttgg caacattgtc taaacgggta taaatgctca tccgctcatt aatgttggtg
     7381 atataagcat ccggaatcag catttccaaa tcggtatcta tttgtacgtc gcgcacatat
     7441 tcctgttttt cttcaatctg gtcggcgaat aatgctttaa attgcgtttg ttttaattcg
     7501 cgaatggctt cgtctaaaat tttatgatac atatcgaagc cgatttccga aataaatcca
     7561 ctttgttcag cgcctaataa attgccggca ccacgaatgt ccaaatcgcg catagaaatc
     7621 tggaaaccac tgcctaaatc agcaaattgt tctatagtgc gcaatctttt tctagaatca
     7681 tcgggtaaac caaataaagg cggagaaatt aaataacaat aggctttttt attacttcta
     7741 cccacacggc ctcgcaactg atgtaaatcg ctcaagccaa accaatgggc gttattaata
     7801 attatggtat ttgcattcgg aatatctaat ccgctttcca caatattggt gcagactaac
     7861 acatcgtaag ttttatcgat aaacttcagc atgtggtctt ccagctcttc gccttccaac
     7921 tgaccatgtg caacaccaat atttaaatcg ggacaaactt ttttaattaa agcagcagtt
     7981 tcctgaatgt cgcgcacccg gttatgaata aaatacacct gaccaccgcg ataaatttcc
     8041 tgataaatgg catcgcgaat aaactcagca tcaaatgttt ttaattcggt ttgaatgggt
     8101 tgacggttag gcggaggtgt attgataata gataaatcac gtgctcccat catactgaat
     8161 tgtaatgtac gtggaattgg tgtagcagtt aaagtaagtg tatcgatatt tgctctgaat
     8221 gcacgtaatt tttctttaac ggtaacacca aatttttgtt cctcatcaat aatgagtaat
     8281 cctaaatctt tgaatttaat ttttttaccg atgatagcat gtgtgccaac aagtatatct
     8341 actttaccgg cttctaattt ttctaaagtt gtttttttat ccttatcaga tttaaaacga
     8401 ttgatataat caaccgtaac aggaaattct tttaaccgtt ctgaaaatgt tttaaagtgc
     8461 tgactagcca gaattgtagt aggaaccaac acagcaactt gtttgccatc ggttactgct
     8521 ttaaatgcag cgcgtatggc aatttcagtt tttccgaatc ccacatcacc acatactaat
     8581 cgatccatcg gatgcggttt ttccatatcg cgttttacat caacagtggc tctgtattgg
     8641 tcaggcgtat cttcgtaaat aaaactagct tctaattcat tttgcaaata gccatccggt
     8701 gaataagcaa atcctttgct ggcttttcgt ttagcataaa gtgcaattaa atctttggca
     8761 atatctttta ctttcgcttt tgttttgcgt tttaattgct cccaggcatc acttcctatt
     8821 ttatttaatt tcggttgcgt gccttctttg cctgcgtatt ttgaaatttt atgtaatgaa
     8881 ttaatattta catataataa atcgttatcg cgataaatta atcgaacagc ttcttgtatt
     8941 tgtcctccga tttcaatttt ttctaaaccc gaatacacgc caacaccgtg atcgatatgt
     9001 gttacaaaat cgccgggacg caattccatc aaggttttta atgtaatggc atcggttttg
     9061 gagaatccgc ttttaaccgt gtatttatgg tgacgttcaa atatctgatg gtcggtaaaa
     9121 caaactattt ttttatcatt atcgataaaa ccggcagcta ttgttgttga tgcagcgcca
     9181 tcttcattaa taaccggaat gatgggtaaa tattttacct gtgcatttaa atcggtaaat
     9241 atttcatgaa aacgttctgc ctgtcgcgca ttactcatga aaataaatag atgataacgc
     9301 agcgattgta attcctttag cttttgaatg agctgctcga aattgcgatt aaacaaaggt
     9361 tgtggtgcag catcaaattg aataatttgt gtatccggta aattactgtg taaaccaaat
     9421 tcaacagcag agaaatgggg cacttcatcc aacaatgcag gtcccgataa caataattcc
     9481 tcaggtggat atttattaaa gggatggtcg cgatcgagta gtttttccct ctttgttact
     9541 tcataccaat ccaacgcctt ttcatatact ttattaatta cctcaaacgt atatttagca
     9601 tctttaaacc aaataatagt gtttttaggc aaggaatgaa atagtgaaga tttttgttct
     9661 gcaccaaact gggtttggat attcggtata atggtaacct gcgaaatggt tttttcagaa
     9721 atttgtgtag taggatcaaa aacgcggata ctttctactt catcgtcata taattctacg
     9781 cgataaggca tttcattgcc aaaagaaaaa atatcgataa taccaccacg caccgaaaat
     9841 tgcccgggtt cgtacacgaa atcggttctt tcaaaaccat tttctgctaa caaatcaata
     9901 ataaaatggt cgtcgagttt ttcacctttt tttaaaagaa taatattttc tttcagcact
     9961 tcggcaacaa caaatttttc gaccaatgct tccgggtaag taacaattat ttctccccgc
    10021 gtatgtgaat tggtaatttt attaatggct tccgtgcgta ataaaacact attggtattt
    10081 acttcttcaa aggaggtttc ttttttaaaa gaatcgggaa taaataaaat gtcttttt
//
LOCUS       DAORKW010000068         9826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_157_length_9826_cov_4.666871, whole genome shotgun sequence.
ACCESSION   DAORKW010000068 DAORKW010000000
VERSION     DAORKW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157_length_9826_cov_4.666871"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1249
                     /locus_tag="PLB46_04475"
     CDS             191..1249
                     /locus_tag="PLB46_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75803.1"
                     /translation="MSHQLAKWFIAGILLATLFSCESDFDINGDYIEKVVVFGLLDYG
                     EDTNFIRIEKTFLEANTNAILLAANSEQIFYGNNELEVYLEEWNNGIYQRTIPVTYVD
                     GDSLGLEKEEGVFSTSPNILYRITTPLDSTSAYTIYIIKTSTEDTITATTQLVQNYHL
                     YFPTYTTSYVNFADTGKMTYTCKQAVNAMLYELTMDFNYYEKNVITGDSVLKTINWQI
                     FNNKEGDNIEGNGNISYVVLRHAFFNFLASSIDENPDVIRTAVSVNYHWYAGGKEVYD
                     LYLNMLANVGINQEYISPEYTNVNGGIGLFSSRHGQHVMDLSLYEATIDSIACGSITG
                     NLRFLSSPANPAYPGCSF"
     gene            complement(1263..2339)
                     /locus_tag="PLB46_04480"
     CDS             complement(1263..2339)
                     /locus_tag="PLB46_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75804.1"
                     /translation="MNKWLVAAGITIAAFSNSCSTDFDLNADFIETPVVYGLLDASVD
                     TQYIRINRAYLSDEVDGLTMATDPESIYYGDELQVRIEEYNFGTLINTYPVERVDGDT
                     LGMPKQEGIFANSPNILYRFVADLDYTHSYKLVAENTETGKIITAETALIDSFAIIRP
                     DDENVFPFSVGLSPQNTFQLRWKSAQDAKIYDLTMRFHYREGIYYPEGDSIHYTYSGY
                     KDWVIDRNVETESTDGNIIITYDVPGETFFNFIKSEFPPVADFSFIRIADSVQFFIDG
                     GGQELFTYFEYNNATLGITEGQITQAYSNVNGGLGILSSRFHKEGKIYPLTLQTRDSI
                     ACSVVTGGLNFAPDGATFGFPYCD"
     gene            complement(2757..>3935)
                     /locus_tag="PLB46_04485"
     CDS             complement(2757..>3935)
                     /locus_tag="PLB46_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRF75805.1"
                     /translation="GTTTLGTYDRFTSRINVPVVVAGSFITGINSEAVGNASPTNTNT
                     GIGVYGKCVNNNYYGYGGYFYGDYAGTFSLGSGYNSLSYGVLALAENVDGYAYGVYGG
                     ASSDANAYNYGVYGFSDQGWIYNVGVTGIQPYYAGAAGYFDGDLEYTGNLYDVSDAKF
                     KSDVKDLTSALDKINMLSPKSYTLNQEQFKGLNFGKTSEFGFIAQEIEAIFPELVKDC
                     VAPVNPYEMGKEPSLEPFKYKAVNYSGLIPVLTAAIQEQQVLIESQNSTITNQETEIA
                     DLKSTVEDLVKRVALIEARNATNIELGDNSMSWLGQNTPNPFGASTQIAYYIPEQSKT
                     AKMIVSDINGKQITTTDIHVFGNGQVNIQSEKMASGNYVYSLVVDDVIVDSKQFSVTK
                     "
     assembly_gap    3936..4035
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4036..4282)
                     /locus_tag="PLB46_04490"
     CDS             complement(<4036..4282)
                     /locus_tag="PLB46_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75806.1"
                     /translation="MKKQLFTTAAALLIAANMFGQNWLTTGNSITGTEKLGTTNNKSL
                     DIITNNSVRMKINNKGNIGVGTTTLGTYDRFTSRINVP"
     gene            4683..6584
                     /gene="dnaK"
                     /locus_tag="PLB46_04495"
     CDS             4683..6584
                     /gene="dnaK"
                     /locus_tag="PLB46_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRF75807.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVIPNDEGRRTTPSIVAFLD
                     NGERKVGDSAKRQAITNPRKTISSIKRFMGHRYSEVSNELSMVSYDVTRGDNDTARVK
                     IEDRLYTPQEISAMVLQKMKKTAEDYLGTEVTEAVITVPAYFNDSQRQATKEAGEIAG
                     LKVKRIINEPTAAALAYGMDKRHKDMKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDKKIMDWLADEFKKEEPTIDLRKDPMALQRLKEASEKAKVELSSSMETEI
                     NLPYITAIDGVPKHLVKKISRSKFEQLADDLIQRTLRPCEAALKDAGYSKNEIDEVIL
                     VGGSTRIPKIQEVVEQYFGKKPNRSVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPL
                     SLGIETLGGVFTRLIESNTTIPSKKSEVFSTAADNQPSVEIHILQGERSMAKDNRTIG
                     RFILDGIPPARRGTPQIEVIFDIDANGILHVTAKDKGTGKEQSIRIEASTGLSDDEIK
                     RMRDEAKANEETDKLAKEKIEKLNRADGMIFETEKQLNEFGDKLSDGAKQPIQDALNE
                     LRDAHKAQDIAACDAATEKLNTAWNNASQEMYAAQQQAQPDAGAGATDNNEQTTANKT
                     DDVEFEEVK"
     gene            complement(7060..9267)
                     /gene="katG"
                     /locus_tag="PLB46_04500"
     CDS             complement(7060..9267)
                     /gene="katG"
                     /locus_tag="PLB46_04500"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HRF75808.1"
                     /translation="MENNGNNGGKCPFTGAMQKHSVGTSGTKNRDWWPNQLKINILRQ
                     NSNLSNPLGAEFNYAQAFKELDLDAIKQDLHHLMTDSQDWWPADFGHYGPLFIRMAWH
                     SAGTYRTGDGRGGAGNGQQRFAPLNSWPDNVNLDKARRLLWPIKQKYGQKISWADLMV
                     LAGNVALESMGFKTFGFAGGRPDVWEPEEDVFWGAETKWLEADKRYSGERDLENPLAA
                     VQMGLIYVNPEGPNGNPDPLAAAKDIRETFARMAMNDEETVALIAGGHTFGKTHGAAA
                     ATHVGPEPEAAGIEEQGFGWNNSYGSGKGGDTITSGLEVTWTQTPAKWSFYFFDNLFK
                     YEWELSKSPAGAHQWIAKNADAVMPDAHDPNKKHVPTMLTTDLSLRFDPIYEKISRRF
                     YEYPLEFADAFARAWFKLTHRDMGPRSRYLGKDVPSEELIWQDPIPAVNHVLVDGNDI
                     QLLKEKISQSGLTISQMVSTAWASASTFRGSDKRGGANGARIRLAPQKDWEVNNPTQL
                     KMVLGILENIQQEFNASQLNGKKISLADLIVLAGCVGVEIAARNGGVAINVPFTPGRM
                     DASQEQTDIESFEALEPAADGFRNYLRKRYTISQAELLIDKSQLLRLTAPEMTVLLGG
                     MRVLNTNFDGSSNGVFTHRPGVLSNDFFSNLLDMNTAWVPTNETKDLFDGHDRKVGGV
                     KWKATQVDLIFGSNSELRALAEVYASADAQMKFVTDFVAAWDKVMNLDRYDLR"
     gene            complement(9320..>9826)
                     /locus_tag="PLB46_04505"
     CDS             complement(9320..>9826)
                     /locus_tag="PLB46_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRF75809.1"
                     /translation="CFYLKANHSRLIFIIFMTITDIQQQLSEKGLKVTPQRIAVLEAI
                     IKLNNHPSAENIIDYIRKNYPNVSTATVYKVLDALVDNELIKRVKTDKDVMRYDAILH
                     AHHHLYCTKTDKIKDYFNDDLNHLLANYFKKHRILDFEIADIKLQIVGKFTSPKKNAL
                     KKRNSLPK"
BASE COUNT         3115 a         1827 c         1745 g         3039 t
ORIGIN      
        1 tggcgagcaa caattggttt cccagctccg cgcgctcaaa acatactata tcgcacatcc
       61 tacaatccca acctctccgc gcctccgcgt gcaactgcat tgtgccattt gtttgccatc
      121 tttttgttat cgctgtgtca tttattaatt ttactattca caacatccaa caggctatta
      181 tatttgatta atgtcacatc aattagcaaa atggtttatt gcaggaatat tgcttgccac
      241 attattttca tgtgaaagtg attttgacat taatggcgat tatatcgaaa aagtagtagt
      301 attcggatta ttagattatg gcgaagacac caatttcatc cgcattgaaa aaacgttttt
      361 agaagccaat actaatgcga tattgttagc tgctaattca gaacaaatat tttatggcaa
      421 taatgaatta gaagtgtatt tggaagaatg gaataacggg atttaccaac gcaccattcc
      481 tgttacttat gtggatggcg attcacttgg tttagaaaaa gaagaaggtg tattttctac
      541 ttcacccaat attttatatc gcattactac accattagac agtacgagtg cttataccat
      601 ttatattatt aaaacatcca cagaagatac gataacagcc accacacaac ttgttcaaaa
      661 ttatcatttg tatttcccca cctataccac ctcgtatgtg aatttcgcag ataccggaaa
      721 aatgacctat acatgcaaac aagcagtaaa tgctatgttg tatgaattaa caatggattt
      781 taattattat gaaaaaaatg taattacagg cgattctgtt ttaaaaacga ttaattggca
      841 aatttttaat aataaagagg gcgataatat tgaaggcaat ggcaatatca gttatgtggt
      901 attacgccat gcatttttta attttttagc atcaagcatt gatgaaaatc ccgatgtgat
      961 tcgcacagcg gttagtgtta actatcattg gtatgcaggg ggaaaagaag tatatgattt
     1021 atacttgaat atgctggcaa atgtgggaat taatcaggaa tatatttcac ctgaatacac
     1081 caatgtaaat gggggaatag gattattttc atccaggcat ggacagcatg taatggatct
     1141 gtcgttatat gaagcaacaa tagattctat tgcctgcggc agtattaccg gtaatttaag
     1201 atttctgtca agtccggcta atccggctta tccaggttgc tcgttttaat aaaagttctg
     1261 tattagtcgc aataagggaa tccaaatgtt gcaccatcag gggcaaaatt taatccccct
     1321 gttactacac tgcaagcaat ggagtctcta gtttgtaaag ttaaaggata aatttttcct
     1381 tctttatgaa aacggctgga taaaatgcca aggcctccgt ttacatttga atatgcttga
     1441 gtaatttgac cttcagtgat acctaaagtg gcattattat attcaaaata tgtaaacaat
     1501 tcttgaccac caccatcaat aaaaaattga acagaatctg ctatacgaat aaaactaaaa
     1561 tcagcaaccg gtgggaattc ggattttata aaattaaaaa atgtttcacc cggcacatca
     1621 taagtaataa taatattacc atcagtggat tcagtttcaa cattgcggtc aattacccaa
     1681 tctttatatc ctgaatatgt ataatgaata ctgtctcctt ccggataata aattccttca
     1741 cggtaatgga aacgcatggt taaatcataa atttttgcat cctgagcact tttccaacgc
     1801 aattgaaaag tattttgtgg tgataatcca acggaaaaag gaaatacatt ttcatcatca
     1861 gggcgaataa ttgcaaagct gtcgattaat gcagtttctg cggtaatgat tttgccggtt
     1921 tcggtgtttt ccgcgactaa tttataagag tgggtataat ctaagtctgc aacaaaacgg
     1981 taaagtatgt ttggtgagtt ggcaaaaatc ccttcctgtt taggcattcc taaagtgtcc
     2041 ccgtctactc gttcaaccgg gtaagtattg attaaagtcc cgaaattgta ttcctcaata
     2101 cgcacttgaa gttcatcgcc gtaataaatg gactcagggt cagtggccat agttaagcca
     2161 tcaacttcat cgcttaaata agcacggttg attctgatat attgtgtatc taccgaagca
     2221 tccaataagc catacaccac aggggtttca ataaagtcag cgttcaggtc aaaatcggtt
     2281 gaacaactgt ttgaaaatgc agcaattgta atacctgctg ccactagcca tttattcata
     2341 agctgcaaag atagcagttt aaccaattga tatgtaaaat gattgtcagg aatacacgaa
     2401 taacagatgt aaagtgcctg ttttaacggt ttttagcatt ttattatcgc ggctttattg
     2461 ttaaaaagca aaatggcccc aaaacggagc catcttttag ggtaagggtt aggggtttag
     2521 atagtaacgc tcaataattt tgaagccata ataatatcat caacaaccag ggtttaaatg
     2581 taatttccgg tattaaaatt tggtgcatgt atagtcatgg taccatcgac aaaacctatc
     2641 attctctttt agtatcaata tgtgcggttt tgtaattata aaaactcaaa attgtcatat
     2701 aacgtaaatt aaaaagtgac tgtctgtaca gccacttttt aatcagggga aatagattat
     2761 ttcgtaacac taaattgttt gctgtcaaca ataacatcat caacaactaa tgaataaaca
     2821 taattgccag aagccatttt ttcagattga atatttacct ggccgttgcc gaaaacatga
     2881 atgtcagttg tagtaatttg tttgccgttg atatcactta caatcatttt agcagtttta
     2941 gattgttcag gaatataata ggctatttgt gttgatgcac caaatggatt aggtgtgttt
     3001 tgacctaacc agctcattga attgtcacct aattcgatat tagttgcatt tcttgcttct
     3061 attaatgcca cgcgttttac taagtcttca acagttgatt ttaaatcagc aatttcagtt
     3121 tcctgattgg tgatggtgct gttttgtgat tcaattaata cttgttgttc ctgaatagca
     3181 gcagttaata caggaattaa gcctgaataa tttactgctt tgtatttaaa tggttcaaga
     3241 cttggttctt tgcccatttc ataagggttt acaggagcaa cacaatcttt cactaattca
     3301 ggaaaaatgg cttcaatttc ttgagcaata aatccaaatt cgctggtttt accaaaattt
     3361 aaccctttaa attgctcttg atttaaagtg taactttttg gtgacaacat atttattttg
     3421 tctaaagcgc tggttaaatc tttaacatct gatttgaatt ttgcatcact cacatcatat
     3481 aaatttccgg tatattctaa gtctccatcg aaataaccgg cagcacctgc ataataaggt
     3541 tgaattcctg ttacacctac attataaatc cagccttggt cactaaaacc atatacacca
     3601 taattgtaag cattagcatc agagctggcg ccaccataaa caccatacgc atatccatca
     3661 acattctctg ccaatgcaag tacaccgtat gataatgaat tgtacccgct acctaaagaa
     3721 aaggtgccgg cgtaatcacc ataaaaatat ccaccataac cataataatt attgtttacg
     3781 catttaccat acacaccaat gccggtgtta gtattagtag gactggcatt tccaactgct
     3841 tcactgttga tgccggtaat aaatgaaccc gctacaacta ccggaacatt aattctggag
     3901 gtaaaacggt cgtaagtgcc caatgtagtt gttccnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnccgga acattaattc tggaggtaaa acggtcgtaa gtgcccaatg
     4081 tagttgttcc aacaccaata tttcctttgt tgttgatttt catacgtaca ctgttgttgg
     4141 taataatatc caacgatttg ttgttcgtag ttccgagttt ttcggtacct gtaatggagt
     4201 ttcctgttgt cagccaattt tgaccaaaca tgtttgccgc tataagtaaa gcagctgcgg
     4261 tagtaaataa ttgttttttc ataacgatta atttttgggt tttgttaaaa aagtatttgt
     4321 tgatttgttt tgatgaggca aaaatatttt ccatctaaaa agtcaccaag aggtttttgg
     4381 aaaattatta aatttcaagt atgttaatat gttttataat atactgataa tcaaataatt
     4441 aaatttcaaa agacttaaaa gttaaggcat caatttagcg ccattaaaag ctaaaaaccc
     4501 tataagcctt cggtcataca gaaagccgat ataatgtcat ccggaatgag gaattagcta
     4561 aatgttgtcg catattccat ccacaaacca acaaaacctg tcagcctgtc agttttatgc
     4621 caatggtata aaaattgtcc accgccaatc attaaaactt gtaaacaaac aatctttata
     4681 caatgggaaa aatcataggt atagacttag gaacaacaaa tagttgtgtt tcggtaatgg
     4741 aaggaaacga accggttgtt attccaaacg atgagggccg cagaacaacg ccttctatcg
     4801 ttgcgttttt agataacggt gaacgtaaag ttggagattc agctaaacgt caggcaatta
     4861 ccaatcctcg taaaaccatt agctctatca aacgttttat gggacatcgc tatagcgaag
     4921 tgagtaatga gttaagcatg gttagttatg acgtaactcg tggcgacaac gatactgcac
     4981 gtgttaaaat tgaggaccgc ttatatacac ctcaggaaat ttcagcaatg gtattgcaga
     5041 aaatgaaaaa aactgcagaa gattatcttg gcactgaagt tacggaagca gttattaccg
     5101 ttcctgctta ctttaacgat tctcagcgcc aggcaactaa agaagccgga gaaattgcgg
     5161 gattaaaagt aaaacgtatc atcaatgaac caaccgctgc tgctttagca tacggtatgg
     5221 ataaacgtca caaagacatg aaaatcgcag tattcgacct tggtggaggt actttcgata
     5281 tttcaatttt ggaattgggt gatggtgtat ttgaagtaaa atctaccaat ggtgatacac
     5341 acttaggagg tgacgacttc gataaaaaaa ttatggattg gctggcagat gaattcaaaa
     5401 aagaagagcc aactatcgat ttacgtaaag acccaatggc tttgcaacgt ttaaaagaag
     5461 cttcagaaaa agcaaaagtt gaattgtctt catcaatgga aacagaaatc aacttgcctt
     5521 atattacagc tatcgacggt gtgccaaaac acttggtgaa aaaaatcagc cgttctaaat
     5581 ttgaacaact tgctgatgat ttaattcaac gcactttacg tccttgcgaa gcagcattaa
     5641 aagatgccgg ttatagcaaa aacgaaattg acgaagttat tttagtaggt ggttctacac
     5701 gtattcctaa aattcaggag gttgttgaac aatattttgg taaaaaaccg aatcgcagtg
     5761 tgaaccctga tgaagttgtt gccgttggtg ctgcaattca gggtggtgta ttgacaggcg
     5821 aagtaaaaga tgtattgtta ctcgacgtta caccattatc attaggtatc gaaactttag
     5881 gtggtgtatt tacacgatta atcgaatcga atacaactat tccttctaag aaaagcgaag
     5941 tgttttcaac tgctgccgac aatcagcctt ctgttgaaat tcatatttta caaggtgagc
     6001 gttcaatggc aaaggataac agaacaattg gtcgctttat attagatggt attccacctg
     6061 caagaagagg aactccgcaa attgaagtta ttttcgatat cgatgcgaat ggtattttac
     6121 atgttactgc aaaagataaa ggaacaggta aagaacaaag tattcgtatt gaagcttcaa
     6181 ccggcttaag cgatgatgaa attaaacgca tgcgtgatga agctaaagcg aatgaagaaa
     6241 ctgataaatt agctaaagaa aaaatcgaaa aactcaaccg tgcagacggt atgattttcg
     6301 aaactgaaaa acaattaaat gagtttggtg ataaactgag tgatggtgct aaacaaccaa
     6361 tccaggatgc attaaacgaa ttgcgtgacg cgcacaaagc tcaggatatt gcagcttgtg
     6421 atgcggctac tgaaaaactg aatacagcat ggaataatgc aagtcaggaa atgtatgcag
     6481 cacaacaaca agcacaacct gatgcaggtg caggcgctac agataataat gaacaaacaa
     6541 ctgcaaacaa aacagatgat gttgagtttg aagaagtgaa gtaattataa ttaattaatg
     6601 aaataattaa acccgttgag caatcagcgg gttttttgtt ttacgcggag aattaataaa
     6661 ttaaaaataa ttacatttta aattgtttta ccacctccgc gactccgcgt gaaccagata
     6721 aaaaatgttt ctcgcaaagg cccaaaggca aagacagatt aatttatgtt tatataaaaa
     6781 accaattcaa gcgcgcaaag gtataattgg tttacctgct ttgcgcactt gaaaacaaac
     6841 catatctcac aatatctaaa taaaaaaaaa cttagcgcct tagcgagaaa caaacactat
     6901 acgtttctca caaagacgct aattaataaa ggtatataag taaaactaaa tactcaattc
     6961 acgtttaaat ggtttaccaa ctccgcgcgc tcaaatacaa accacatcgc acacccaaca
     7021 atcacaaacc ctccgcccct ccgcgtgaaa ccccaccgat tatctcaagt cgtatctatc
     7081 caaattcatc accttatccc aagctgcaac aaaatcagta acaaatttca tttgcgcatc
     7141 tgcactcgca tatacttcag ctaaggcacg gagttctgaa ttagagccaa aaattaaatc
     7201 tacctgagtt gctttccatt ttacaccacc tacttttctg tcgtgaccat caaataaatc
     7261 ttttgtttca tttgtcggta cccaggcagt attcatatct aataaattac taaaaaagtc
     7321 gttgcttaat acacccggac gatgagtaaa taccccattt gatgaaccat caaaattcgt
     7381 atttaataca cgcatcccgc ctaataaaac agtcatttca ggagcagtta atcgcaataa
     7441 ttgtgattta tcaattaata attcagcctg agaaatggta taacgttttc ttaaataatt
     7501 gcggaaacca tcagcagcag gttcaagcgc ttcaaacgat tcaatatctg tttgttcctg
     7561 cgatgcatcc attcttcccg gtgtaaatgg cacgttgata gcaacaccac cattgcgtgc
     7621 agcaatttca acaccaacac aacctgctaa tacaattaaa tccgctaatg atattttttt
     7681 accgttcaat tgtgatgcat taaactcttg ttggatattt tctaaaatac ctaacaccat
     7741 ttttaattgt gttggattat ttacttccca atctttttgc ggggcaaggc gaatacgtgc
     7801 gccatttgca ccaccacgtt tatctgaacc gcggaatgtt gaagcagaag cccatgcagt
     7861 agataccatt tgcgaaatgg ttaatccaga ttggctgatt ttttctttca atagttgaat
     7921 atcgttacca tccactaaaa catggttgac agcaggaatt gggtcttgcc atattaattc
     7981 ttctgaagga acatctttac ccaaatatct tgagcgaggt cccatatcac gatgtgttaa
     8041 tttaaaccat gcacgtgcaa atgcatctgc aaattcaaga ggatattcat aaaaacgacg
     8101 agatattttt tcataaatcg gatcgaatct caacgataaa tcggtagtca acatagttgg
     8161 cacatgtttt ttatttgggt catgtgcatc cggcataacc gcatctgcat tttttgcaat
     8221 ccattgatga gctcctgcag gacttttaga taattcccat tcatatttaa ataaattatc
     8281 gaaaaagtaa aaactccatt ttgcaggtgt ttgtgtccag gtaacttcta atccactggt
     8341 aatagtatcg ccaccttttc ctgagccata actgttattc cagccaaaac cttgttcttc
     8401 aattcctgca gcttcaggtt caggaccaac atgtgttgca gcagcggcgc catgtgtttt
     8461 accaaaagta tgtccgcctg caattaatgc tactgtttct tcatcattca ttgccatacg
     8521 agcaaatgtt tcacgaatat cttttgctgc tgcaagtggg tcgggattac cattaggacc
     8581 ttccggattt acataaatta atcccatttg cacagcagct aacggatttt ctaaatcgcg
     8641 ttcacctgaa tatcgtttat ctgcttccag ccattttgtt tcagcgcccc aaaaaacatc
     8701 ttcttcaggc tcccacacat caggtcttcc accggcaaac ccgaaagttt taaaacccat
     8761 tgattctaat gcaacgtttc ctgctaatac cattaaatcg gcccatgaaa ttttttgacc
     8821 atatttttgt tttattggcc acaataatct gcgtgcttta tctaaattca cgttatcagg
     8881 ccaactgttt aatggtgcaa aacgttgttg cccgttgcct gcgccaccac gtccatcgcc
     8941 tgtgcgatac gttcctgcac tgtgccatgc catacgaata aataatggac cataatgtcc
     9001 aaaatctgcc ggccaccaat cctgtgaatc ggtcatcaaa tgatgcaagt cttgtttaat
     9061 tgcatctaaa tccaattctt taaatgcttg cgcataatta aattcagcac caagtggatt
     9121 cgataaattg ctgttctgac gcaatatgtt tattttaagt tgattaggcc accaatcgcg
     9181 gttttttgtg ccgctggttc ctacactgtg tttttgcatt gctccggtaa aagggcattt
     9241 gcctccgttg tttccgttat tttccattgt attgtgatat ttatattttc aattatttat
     9301 actctatgtt ttattgtgcc tatttcggga gggaattacg ttttttaagt gcgttttttt
     9361 tgggagatgt aaatttgcca actatctgta atttaatatc tgcaatttca aaatcaagga
     9421 tacgatgttt tttgaaataa ttagctagaa ggtgatttaa atcatcgtta aaataatctt
     9481 tgattttatc tgttttggtg cagtataaat ggtggtgagc atgtaaaatg gcatcatacc
     9541 gcattacgtc tttatctgtt tttacacgtt taattaattc attatctact agtgcatcta
     9601 atactttata aaccgttgct gttgaaacat taggataatt tttacgaata taatcgatga
     9661 tgttttctgc ggaagggtgg ttgttgagtt ttatgatagc ttctaaaacg gctattcgct
     9721 gtggggtgac ttttaaccct ttttcactta gttgttgttg aatgtcggtt atggtcataa
     9781 atataatgaa tattaaacga gaatgattcg cttttagata aaagca
//
LOCUS       DAORKW010000069         9802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_158_length_9802_cov_4.269416, whole genome shotgun sequence.
ACCESSION   DAORKW010000069 DAORKW010000000
VERSION     DAORKW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9802
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158_length_9802_cov_4.269416"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..869
                     /locus_tag="PLB46_04510"
     CDS             60..869
                     /locus_tag="PLB46_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75810.1"
                     /translation="MKLGALFVGMVICFQVLAQDTVVAPQSKSNPIVLNKGNSASKPS
                     GLSYEHEFSMGAKVTTTGWGIFGDYTKDVNYDKKRVMYFELAFLKNPKETKRVNEFTI
                     GFTPPKPFVYGKQNTFFNLKAAIGEKRLIGEKAEKSGFQISANYAGGFSAGFVKPYYL
                     EALNTDAEDGNLTIPIKYSPETDSLFLDATKIYGASGFSAGFNEISFVPGLFGKAGLN
                     FDWATYDDFVKSAETGIGAELYLKEIPIMIQENNKPLFVYLYLSLQFGKKW"
     gene            885..1757
                     /gene="lipA"
                     /locus_tag="PLB46_04515"
     CDS             885..1757
                     /gene="lipA"
                     /locus_tag="PLB46_04515"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRF75811.1"
                     /translation="MIELPVADAHERLRKPKWLKVKLPTGENYRNVRQIVDEFKLHTI
                     CESGNCPNMGECWGEGTATFMILGNVCTRSCSFCAVATGRPTELDLEEPQRVAEAIKL
                     MKVKHAVITSVNRDELADRGASVWHDTVAAIKNLSPGTTIETLIPDVKANWDALITMI
                     SPGQEVVSHNMETVKRLYRLVRPQAKYERSLEQIQRTKAYGKRTKSGVMLGLGETPDE
                     VYAIMDDLLTHGCEILTLGQYLQPTKMHLEVKEFVTPEMFEHYREIGMNKGFLYVESG
                     PLVRSSYHAERHVF"
     gene            1857..4847
                     /locus_tag="PLB46_04520"
     CDS             1857..4847
                     /locus_tag="PLB46_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75812.1"
                     /translation="MKRNFTIFSLSFISLVMLSLLVVSSFGKQESTAFNSDTIKGLHD
                     RAERKHARVEGYRTWLNSMKANPKTGKVEMADVFAAREQMKEYFQQASSGERGGSTLN
                     LAWETMGPTNVGGRTRIILLDKNNPDRMYAGGASGGLFYSNNGGLEWFPHPQNAEFSS
                     LLICAMDQAANGDIYFGTGEYWADYFDGSFGSYTHGFVGDGVFKAPAVTGDELPTFSQ
                     LTATVPTPADLGSTSGVAWAYVNRIACSPLDANIVVAATNTGLKISKDGGNSWENCEG
                     GGSPLSSVSDDAQFDNQGYLHAISSSLRKYYRSTTTADPAVLDELGTGLPVGSTRRVL
                     AVAPSDNNYVYIYSAKSGTYGLQGVYQSTDMGVNFTQITEEASEFFNPNGTGANTTWN
                     MCIAVKPDNPQRIYIGGQIQAWTWNGLGGSWTAMTSSGYPAWYPKYIHADHHFITFHP
                     ENPEIMYYGSDGGVSRSLNASSLYPDFATLNKGLNFYQANGVAIGIEGEAMGGSQDNG
                     TQYVNFDLNSELQSVEVLGGDGGKAEISHIRPEYLFGTFFSITANGNGAALRRSVNEG
                     ASMAAIYDCNIDGGTSNCTQDGLADGGTDFVTPFVLWENYDLFKTFEPVLTGGSVEYP
                     VGSGDFYEIGDEVNYLGRDITLSSTSLYESRLYQAVKTNLWVTTGALFNSTEAPSWFK
                     ILPSTSGTISAIEYDNSGDIVYVGTENGRLYRVSGLLTANFEYVDVDGDPETAGVFNA
                     ATAGITSFTYANIFPGKITGISINRNNPDEIAISIGGYGVDQNVWITDNGLDGDAAIF
                     ECVADGGALPNIPAYDILMLSSDNDKLLIATEFGIWSYSISSGSPWTLENGFTAGEIG
                     PGFVPVFEVREDWVRSKDCLAIYVGTHGNGFYRATNLAGAECDFTTVSSGPIQEEIIA
                     GVTLAPNPADIYTNANIILNDGALIAISIVNLSGAAVKNYGATNYPSGVQNINLDVRD
                     LTPGTYLVVFEINGVLTSKRLVVI"
     gene            complement(5409..7001)
                     /locus_tag="PLB46_04525"
     CDS             complement(5409..7001)
                     /locus_tag="PLB46_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75813.1"
                     /translation="MKKNFLLIVCLIIVTALHAQAPLNYQSSVLQGSEQIRDAKNITK
                     SDVLKQTADQGDRALTYYWLNYSDAVDYAFNAWALENLAALPLWPDSSGYVVTGTGED
                     FFWYGHGYVHMFDPISDFVSDFVDDNFTVIGDAKDWFNADHAFDIDSVRFYYLYERPT
                     TGYTDTLKVYLLAPNSNCYVENLYFDNDANGDFDEGTDISVVLSKYSSAGNKPNGTVT
                     EYTFLLTDADTASENVAFKQMAIDYTITKNSPDHYLGIAFQFIPGQPYSLGDTLLDFS
                     DPPLNITNPLNRFWLLCNEEILESSPASWSEYSENHNGFASTEVRYDLSPGSWNGYYI
                     STYAYTDAFAFEQGYVDWYVAPKGVNFLSSTPSPCVSLTKNFQDLSNFAAVPDDATYY
                     WEFGDGNLSFERNPSHTYLIPGTYTVCLAVDYGGQAYDYCKNVSVDYCTAIEEIENLS
                     VMSLFPNPADDVLNISLEFGSAQDAQISIVNMSGQVVETITTGVITNYSNAIDISGLS
                     AGVYMVRVSSGNQATIRNFVVE"
     gene            complement(7101..7670)
                     /locus_tag="PLB46_04530"
     CDS             complement(7101..7670)
                     /locus_tag="PLB46_04530"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRF75814.1"
                     /translation="MFIEPHLKGERRGWIEVICGSMFSGKTEELIRRLKRARIANLKV
                     EIFKPAVDTRYDVQNIVSHDETAILSSPVSNSLNILLLSEDVDVVGIDEAQFFDEHIV
                     EVCEKLAQKGIRVIVAGLDMDFRGKPFGPMPELLAIADYITKVHAICMQCGNIASYSF
                     RKVHNENQVLLGEKNEYEPRCRNCFLKDS"
     gene            7739..8575
                     /locus_tag="PLB46_04535"
     CDS             7739..8575
                     /locus_tag="PLB46_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75815.1"
                     /translation="MDTKITEIEILLDKLNLLFASIKNDGVIDKVEMELLRKYVQQLQ
                     DKLNTDSGQKIVPPVVIPVAKVEEVKKEVEKTMIPEIEVPVHPVELPKVEKIDEPIVS
                     KEVNVPITKTPVVEPVINPVKEVEKKVEQIIPPVEIEKQKIVETPVVNKSPEVKPPVS
                     GNDKKPLAILDDEEEEEDFNAGLNNKLYKDKKTLADKLTSSKGKDLKSVIDLNEKLFF
                     INKLFKGDKDAYENTIKQLNQVTSYEAGKQLIVADLANKYNWKDKEAIERLTEVIQQK
                     FM"
     gene            complement(8580..>9802)
                     /locus_tag="PLB46_04540"
     CDS             complement(8580..>9802)
                     /locus_tag="PLB46_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRF75816.1"
                     /translation="HAGIRIAALIQPKAKLWVAGRKNIFGKIKQEFVRDNSKVVWFHC
                     ASLGEFEQGRPVMEQLKKDYPHIKIVLTFYSPSGYEVRKNFEGADYVTYLPEDTASNA
                     KQFVELIQPDLVVFVKYEFWYHYLNTLATKKIPTVLISAIFRKKQLFFKWYGRLHKAM
                     LKKFTQIFVQNENSLQLLQSIKIQHVAIGFDTRYDRVFEVAQNVKQYPEIATFTANHN
                     VIVAGSTWAKDEKILADAFYSKLVYVNFKIIVVPHNIDKKSITKTKKRFKKYALVYSE
                     LHKATEADLIGKRVLIVDTIGMLNALYQYADVNYIGGGFNKGIHNTLEAAVYGKPILF
                     GKKYKKFDEAVGLVNVNAALVIRDADELLNRINLMNQFQFIYKGAGSDADKFIKSHLG
                     GTATVMQYLAQLLK"
BASE COUNT         3094 a         1797 c         1943 g         2968 t
ORIGIN      
        1 atgacaattt tttttaaaat taggcaactt atctgcgaca aaattcgtaa tattatccga
       61 tgaaattagg tgcactgttt gtaggtatgg tgatttgctt tcaagtgttg gctcaggata
      121 ctgtagttgc acctcaaagt aaatcaaatc ccatcgtcct aaacaaaggc aattctgctt
      181 caaaaccttc cggattaagc tatgagcacg aatttagcat gggtgctaaa gtaactacca
      241 ccggttgggg tatttttggt gattatacca aagatgtcaa ctacgacaaa aaaagagtaa
      301 tgtattttga attggcattc ctcaaaaacc ctaaagaaac aaaacgtgtt aacgaattta
      361 ctatcggatt tacgccacca aaacccttcg tttacggtaa acaaaacacc ttttttaatc
      421 tgaaagcagc aataggcgaa aaacgattaa taggtgaaaa ggctgaaaaa agtggattcc
      481 aaataagtgc caattacgca ggcggttttt cagccggttt tgtaaaacca tattatctcg
      541 aagctttaaa cacagatgct gaagatggta accttaccat accaattaaa tattcacctg
      601 aaacagacag tttgttttta gatgccacca aaatttatgg ggcctccgga tttagtgccg
      661 gatttaatga aatttccttt gtgcccggat tgttcggcaa ggccggatta aattttgact
      721 gggcaactta cgacgacttt gtaaaatcgg ctgaaaccgg cattggtgct gagttatacc
      781 taaaagaaat tcctattatg attcaggaaa acaacaagcc gctttttgtt tatttatacc
      841 taagtttgca gttcggaaag aagtggtaaa aattacgtaa aacaatgatt gaattaccgg
      901 ttgcagatgc tcacgaaaga ctgagaaaac caaaatggct taaagtaaaa ttgcccaccg
      961 gcgaaaacta ccggaatgtt aggcaaatag ttgatgaatt taaacttcat accatttgtg
     1021 aaagcggaaa ttgtcctaat atgggcgagt gctggggcga aggaacagct acttttatga
     1081 ttttaggcaa tgtttgtaca cgttcctgct ctttttgtgc tgtagctacc ggaagaccaa
     1141 cggaactcga cctcgaagaa ccacaacgtg ttgcagaagc catcaaatta atgaaggtaa
     1201 aacatgctgt aattaccagt gttaatcgtg atgaactcgc cgaccgcggt gcttctgttt
     1261 ggcatgatac tgttgcggca attaaaaatt tatcacccgg cactaccata gaaacactaa
     1321 ttccggatgt aaaagcgaat tgggatgcgc ttatcaccat gatttcaccc ggacaggaag
     1381 ttgtttcaca caatatggaa accgtaaagc gcttataccg tttggtgcga ccacaggcaa
     1441 aatatgaacg cagtctggag caaattcagc gcacaaaagc ctatggcaaa cgcacaaaat
     1501 ccggtgttat gttaggcctc ggtgaaacac ctgatgaagt ttatgctatt atggacgatt
     1561 tgcttactca cggatgcgaa atactaacat taggccaata tcttcagcca acaaaaatgc
     1621 acctcgaagt aaaagaattt gttactcctg aaatgtttga acattatcgc gaaatcggca
     1681 tgaataaagg atttttatat gtcgaaagtg gcccgctggt aaggtcatct taccatgcag
     1741 aacgtcatgt gttttgacat aaaaagccaa ttgtaaaatg taattagtta gtcataaata
     1801 tttacttgtt aattagtctt aatatttttt tatagtttta cccaaaatag cgtttcatga
     1861 aaaggaattt tacgattttc agtctctcat ttattagtct ggtaatgtta tcattgcttg
     1921 tggttagtag ctttggaaaa caagagtcta ctgctttcaa tagtgatact attaaaggtt
     1981 tgcatgatcg tgcagaaaga aaacacgctc gtgttgaagg ttaccgcact tggctaaaca
     2041 gtatgaaggc taaccctaaa acaggaaaag tggaaatggc agatgttttt gctgcccgtg
     2101 aacaaatgaa agaatatttt caacaggcta gcagtggcga gcgcggtggc tcaacattaa
     2161 atcttgcttg ggaaactatg ggaccaacaa atgttggtgg acgtacacgt attattttac
     2221 ttgataaaaa taatccggat agaatgtatg ccggcggcgc aagcggtggt ttattttatt
     2281 ctaataatgg aggtttggaa tggtttccac acccacaaaa tgcagaattc agctctttat
     2341 tgatttgtgc aatggaccag gcagctaatg gtgatatcta ttttggaaca ggtgaatact
     2401 gggccgatta ttttgatggc tcttttggta gctatacgca tggatttgtt ggcgatggtg
     2461 ttttcaaagc tccggcagtt accggcgacg aattacctac ttttagtcaa ttaactgcta
     2521 ctgtaccaac tccagctgat ttaggaagca cctcaggtgt tgcttgggct tatgttaacc
     2581 gaatagcttg tagccctctc gatgctaata tcgttgttgc tgcaactaat accggtttga
     2641 aaatttcaaa agatggtggt aactcatggg aaaactgcga aggcggtgga agccctctca
     2701 gcagcgtttc tgatgatgca caattcgata atcagggata tttacacgca atttcttcca
     2761 gcttaagaaa atattatcgt tcaactacaa cagcagaccc tgcagtgtta gacgaactgg
     2821 gaactggttt acctgtaggc agcacacgtc gcgttttagc agttgctccg tcagataata
     2881 actatgtgta tatctattca gccaaatcag gcacttatgg acttcagggt gtttaccaat
     2941 caactgacat gggtgtaaac ttcacccaaa ttactgaaga agccagtgaa ttttttaacc
     3001 cgaatggaac cggtgcaaac actacttgga atatgtgtat tgcagttaaa ccggataatc
     3061 cacaacgtat ttatatcggt ggtcaaattc aagcttggac ttggaatgga ctgggcggca
     3121 gctggacagc aatgaccagt tctggttatc cagcctggta tcctaaatat atccacgccg
     3181 atcatcattt tatcactttc cacccggaaa atcctgaaat catgtattat ggttcagacg
     3241 gtggtgtttc aagatcttta aatgcatctt cgctttatcc tgatttcgca acacttaaca
     3301 aaggattgaa tttctatcag gcaaatggtg ttgcaattgg cattgaaggt gaagctatgg
     3361 gtggttctca ggataacgga acgcaatatg taaacttcga ccttaactca gaattgcaat
     3421 ctgttgaagt acttggtggt gatggtggta aagcagaaat ttctcatatt cgccctgaat
     3481 atttattcgg aacattcttc tcaataactg ccaacggaaa cggtgctgca ttgcgtcgtt
     3541 cagtaaatga aggcgcatca atggccgcaa tttacgattg taatatcgat ggtggaacat
     3601 ctaattgtac acaggacgga cttgctgatg gtggaaccga cttcgtaaca ccgtttgttt
     3661 tatgggaaaa ttatgattta ttcaaaactt ttgaacctgt tttgacagga ggctctgttg
     3721 aatatcctgt aggtagcggc gatttttatg aaattggcga cgaagtgaat tatttgggaa
     3781 gagatatcac attatcttct acttcattat atgaatccag attgtatcag gcagtaaaaa
     3841 ctaacttatg ggttacaact ggcgcattat tcaattctac tgaagctcca tcatggttta
     3901 aaatattacc ttctacatca ggaactatta gtgctattga atatgataat tcaggagata
     3961 tcgtttacgt aggaacagaa aatggaagat tataccgtgt aagtggttta ttgactgcaa
     4021 actttgaata tgttgatgtt gatggagatc cggaaacagc tggtgtattt aatgcagcta
     4081 ccgcgggtat aacatctttt acctatgcaa atattttccc tggtaaaatc accggtatca
     4141 gtatcaaccg caataaccct gatgaaattg caatttcaat tggcggatat ggcgttgacc
     4201 aaaacgtttg gattactgac aatggtttag atggtgatgc ggcgattttt gaatgtgttg
     4261 ctgatggcgg tgcacttcca aatattccag cttacgatat ccttatgttg tcatctgata
     4321 acgataaatt gcttatcgct actgaatttg gaatttggag ttattcaatt tcaagcggat
     4381 caccttggac tttagaaaat ggctttacag caggtgaaat tggccctggt tttgttccgg
     4441 tgtttgaagt tcgtgaagat tgggtacgca gcaaagattg tttagctatc tatgttggta
     4501 cgcatggtaa cgggttctac cgcgctacta atttagccgg cgcagaatgt gactttacca
     4561 cggttagttc aggacctatt caggaagaaa ttattgcagg tgtaacatta gcacctaatc
     4621 cggcagatat ttataccaac gcgaatatta tcctgaatga tggcgctctt atcgcaatat
     4681 caatagttaa tttgtctggt gccgctgtta aaaactatgg tgcaacaaat tatccttcag
     4741 gtgtccaaaa catcaacctc gatgttcgcg acctaactcc tggaacttat ctggttgtat
     4801 ttgaaattaa tggagtgctt accagcaaac gtttagtagt tatttaatct aactacaata
     4861 tcaatatttc aaggctcctt ccgctatgaa ggagcctttt tttataacct gtaaagccaa
     4921 tggcaggaac ttacaggcca atccgccact tttgctgtaa ctagcctgac aggcgattaa
     4981 tttttcctta tcaacgataa tctcctccta tcatcgcatc aaaagctatc aatccatagc
     5041 cattggcttt gctaaccatc aattcaacca agggtggaag gtgtataatt tatagacata
     5101 gtaattaggt tatctaagcc aaatactgcc aatggctcat ataactaaaa tataatgctg
     5161 gggtagttga tatttaccct tgccaccagt caattgaggg gtgtaaagag cctgatttat
     5221 cagtgaggtt ccggtagata acctgtttaa cctgcagaat gaagtggggg cgctataatc
     5281 ttaggtttga tctaattgca ttatcaaagc caacattaag cttatattaa caactgaaac
     5341 ctaattaaat aggcgaaaaa aaagcccccc aagatgggag gctttttaat atgataagtt
     5401 gcaaagtatt attctacaac aaaattgcgg atagtagctt ggttaccgct tgaaacgcga
     5461 accatataaa caccagctga taagccgctg atatcaatag catttgaata atttgtgatt
     5521 acaccggtag tgattgtttc aacaacctga ccagacatat tcacgataga aatttgtgca
     5581 tcctgagcgc tgccaaactc taaagagatg tttaaaacat catcagcagg gttagggaat
     5641 aaactcataa cacttaaatt ttcaatttct tctattgcag tgcaatagtc aactgataca
     5701 tttttgcaat aatcataagc ttgtccaccg tagtcaaccg ctaaacaaac agtataagta
     5761 cccggaatta ggtaggtatg actagggtta cgttcaaaac ttaagttacc atcaccaaat
     5821 tcccaataat aagttgcatc gtcaggaact gcagcaaagt tggataaatc ctggaagttt
     5881 ttggttaaac ttacgcatgg tgaaggggtt gaactaagga aattcacacc ttttggagca
     5941 acataccaat ccacataacc ttgttcgaag gcaaatgcat ctgtatatgc ataggtgctg
     6001 atataataac cattccaaga acctggcgat aaatcgtaac gcacttcggt actggcaaat
     6061 ccattatggt tttcagagta ttcagaccat gaagcaggtg aactttccaa tatttcttcg
     6121 ttacataaca accagaaacg atttaaagga ttggtgatgt tcagcggagg atcagaaaag
     6181 tccaacaacg tgtcacctaa gctataaggt tgtcctggta taaattggaa ggcaattcca
     6241 agataatgat caggggaatt tttagtaatg gtataatcta ttgccatttg tttaaaggca
     6301 acattttcac ttgctgtgtc ggcatcggtc aataagaaag tgtattcagt aactgttccg
     6361 ttcggtttgt tgccggcaga agaatattta gagagaacta cactaatgtc ggtaccttca
     6421 tcaaagtcac cgttagcatc attgtcaaag tagaggtttt ctacataaca atttgaattt
     6481 ggagctaaaa ggtaaacttt taaagtgtcg gtatagcctg tggttgggcg ctcatataag
     6541 taataaaaac gcactgaatc gatgtcgaac gcatgatcgg cgttgaacca atctttagca
     6601 tcgccaataa cggtaaagtt atcatccacg aaatctgaaa cgaaatcaga aattgggtca
     6661 aacatatgaa cgtagccatg tccgtaccag aaaaagtcct caccggttcc ggtaacaacg
     6721 tagcctgatg aatccggcca taatggaagt gcagcaaggt tttcaagtgc ccaggcatta
     6781 aatgcataat ccacggcatc tgagtaattt aaccaatagt aggttaacgc tctgtcacct
     6841 tggtcggcag tttgttttaa tacatcgctt ttagttatgt ttttagcatc acgaatctgc
     6901 tcagagcctt gtaaaactga actttgataa ttaagaggag cttgtgcatg caaagctgta
     6961 acaattatca aacagacaat aagtaaaaaa ttttttttca tagctgttct tttgttgaat
     7021 tgtttatttt atttacaaat ataggcatta atagcggaaa atggtcatat aattgcaagt
     7081 taacttagtt tgggttgagt ttaactatcc ttaagaaaac agttcctgca acgtggttca
     7141 tattcatttt tttcaccgag tagtacctgg ttttcattgt gcactttgcg gaaagagtag
     7201 gaggctatat tgccacattg catacatata gcatgaactt tagtaatata atcggctatt
     7261 gcgagcaact ccggcatagg gccaaagggt tttcctctga agtccatgtc aagccctgct
     7321 actataaccc gaattccctt ttgtgcaagt ttttcacata cttctacaat gtgctcatca
     7381 aaaaactggg cttcgtcaat acctaccaca tctacatctt cagaaagcaa tagaatattt
     7441 agggaattag aaacaggtga agataaaatt gccgtttcat cgtgactgac aatgttttgc
     7501 acatcatatc tggtgtctac ggcaggttta aatatctcaa cctttaaatt ggctatacgc
     7561 gcacgtttaa gcctgcgaat cagttcttcg gttttgcccg aaaacatgga tccacaaatt
     7621 acttctatcc atccacgacg ttctcctttt agatgcggtt caataaacat acatttgtac
     7681 tgtatttttc tggtacgaaa ttacgtttta cttatcagtt aaaactactt atttgaatat
     7741 ggatactaaa attactgaaa tagaaattct gctcgacaag ctcaatctgt tgtttgcctc
     7801 aatcaaaaac gacggtgtta tagacaaagt ggaaatggaa ttactaagga aatatgtgca
     7861 gcagctgcaa gataagctca ataccgattc cggtcaaaaa atagttccac cggtggttat
     7921 acctgttgct aaagtagaag aagtgaaaaa agaggtagaa aaaactatga tacctgaaat
     7981 tgaggtacct gttcatcctg ttgaattacc taaggtggaa aaaattgatg aaccaatagt
     8041 tagcaaggaa gtaaatgtgc caattacaaa aacacctgtt gttgaacctg ttattaatcc
     8101 ggttaaagag gtagaaaaaa aggttgaaca aattattcct ccggtagaaa tcgaaaaaca
     8161 aaaaatagta gaaacaccgg ttgtaaataa atcgccggag gtaaaaccac ccgtttcagg
     8221 aaacgacaaa aaacctttag cgattttaga tgacgaagaa gaggaagaag actttaatgc
     8281 cggtttaaat aataaattgt acaaagacaa aaaaacactt gcggataaac tcacttcatc
     8341 aaaaggcaaa gacttgaaga gcgttattga tttaaacgaa aaattatttt ttatcaataa
     8401 gttatttaaa ggtgataagg atgcttatga aaacacaatt aagcaactta atcaggttac
     8461 cagttatgaa gcaggcaaac aattgattgt tgctgatttg gcaaataaat ataattggaa
     8521 agataaggaa gctattgagc gtttaacaga agttattcag caaaaattca tgtagtcggt
     8581 cacttaagta attgagctaa atattgcatc acagtagctg ttccaccaag atgcgatttg
     8641 atgaatttgt ctgcgtcgga acctgcgcct ttataaataa actgaaattg gttcataaga
     8701 ttaattctat tcagtaattc atctgcatct ctgataacta atgcggcatt tacgtttacc
     8761 agacctaccg cttcatcaaa tttcttgtat tttttaccaa ataaaattgg cttgccgtat
     8821 actgcagctt ctaatgtgtt atgaatacct ttattaaaac ctccgccaat ataattcaca
     8881 tctgcatatt gatacaatgc attcagcata ccaatggtat caactattaa taccctttta
     8941 ccaattaaat cagcttcagt tgctttatgc aattctgaat atactaaagc gtacttttta
     9001 aaacgttttt ttgtttttgt tatacttttt ttatcaatat tgtgcggaac aacaattatt
     9061 ttaaagttga cataaactaa tttactataa aaggcatcag ccagaatttt ttcatccttt
     9121 gcccatgtac tacctgctac aattacattg tgattggcag taaatgtggc tatttcggga
     9181 tattgtttta cattttgtgc aacttcaaat acacgatcgt aacgtgtgtc aaatccaata
     9241 gcaacatgct gaatttttat agattgtaaa agttgtaatg aattttcgtt ttggacaaaa
     9301 atttgtgtga attttttaag catagccttg tgtaatcggc cataccattt aaaaaacaat
     9361 tgtttttttc tgaaaattgc ggagattaaa actgttggga tttttttggt ggccagcgta
     9421 tttaaatagt gataccaaaa ttcatatttg acaaaaacaa ctaaatcggg ttgaatgagt
     9481 tcaacaaatt gttttgcatt agatgcagta tcctccggca gataggtaac atagtcggcg
     9541 ccctcaaaat ttttacgcac ctcatatccg gatggagaat aaaacgttaa aacaattttt
     9601 atgtgcgggt agtctttttt aagttgctcc atcacgggtc ggccttgctc aaattctcct
     9661 aaggaagcac aatgaaacca aacaactttg ctgttatcgc ggacaaattc ttgtttaatt
     9721 tttccaaaaa tattttttct tcctgctacc cataatttcg ccttaggttg aatgagcgca
     9781 gcaatgcgaa tacctgcatg at
//
LOCUS       DAORKW010000070         9797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_159_length_9797_cov_6.623281, whole genome shotgun sequence.
ACCESSION   DAORKW010000070 DAORKW010000000
VERSION     DAORKW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159_length_9797_cov_6.623281"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1528
                     /locus_tag="PLB46_04545"
     CDS             131..1528
                     /locus_tag="PLB46_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRF75817.1"
                     /translation="MGKLSLNGKDLRKIGYPESKVIGIAIQVMHQYYKHSAEAEAIDI
                     LKDVLAAPDAYLEDAALGKIAAALLDEIRPKNDVIPLEQKRKEYAIYGFENIEMGAIN
                     QMEVAMKLPVTVAGALMPDAHQGYGLPIGGVLATNNAVIPYGVGVDIGCRMCLSVFDI
                     PGDFINDNVEKLKGFLTRNTVFGAGGTLKNPMDDAVLERKEFSENKLLKSLHDKAFKQ
                     IGTSGSGNHFVEFGIIEIKQSTEKFYIPPGNYLGLLSHSGSRGLGAAVANYYTRLARE
                     LCKLPDVAQHLAYLDLNSAEGQEYWAAMNLSGDYASACHHQIHKKIARSVGAQVLGMV
                     ENHHNFAWKEMYNGQEVIVHRKGATPAGKGVLGIIPGSMTAPGYIVEGNGIANSIQSA
                     SHGAGRKMSRTAAKNNFNQKMLDEKLNEAGVHLIGGDIDESPMAYKDINEVMQYQNEL
                     VNIIGMFKPKVVRMA"
     gene            1801..2304
                     /locus_tag="PLB46_04550"
     CDS             1801..2304
                     /locus_tag="PLB46_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75818.1"
                     /translation="MNILFNIEEKMIRVNKYLYENKCIKAKRLLEDILEEEPDCGSAH
                     FEMGMLYYNELAEYDTALYHFDLCKRFKPGFIPVYHVTVQLLYQLNKHETLIKYANSC
                     LQIEGVNKFFVYKHLAMSCETTLLFKPAIKYYKQSKLYAIHEYDITFIKNSLIRVKEK
                     TVNKSRK"
     gene            complement(2333..2845)
                     /locus_tag="PLB46_04555"
     CDS             complement(2333..2845)
                     /locus_tag="PLB46_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75819.1"
                     /translation="MEPLDNLELKKFKTDKGAVIPAPLGVSAIFTSLIVMFTGHGNLF
                     FVGIFLVGIVILFLKNGVEVDLVNHQYRDYKLLFGNYWGKWKPLAEGDYLLISKSTKR
                     SAAPITMAMSATSSRVFYDLDLITKGSRDVILKRDPDYDKLLRIAKMVADDHALKIAE
                     RVNNDYIWLN"
     gene            3101..3325
                     /locus_tag="PLB46_04560"
     CDS             3101..3325
                     /locus_tag="PLB46_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75820.1"
                     /translation="MSINLKYLIKSSDPEEIYNFVASANFALKLIKAGKGSKAKRYGN
                     AYFFAKTNEDMDSSDFAIETGKLINATKHQ"
     gene            3931..6720
                     /locus_tag="PLB46_04565"
     CDS             3931..6720
                     /locus_tag="PLB46_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75821.1"
                     /translation="MKNNTTFTTQNKIGLLSILIISLALATASSNTNSVFILNNNKIE
                     NKIKINPDTLPDVIDTINSYEQGDTIALDLAGLDTLFILFEDTTFAGSDTLSDTIALR
                     LFDVTKDITITPGAITKTVAPGLFGIHIEGIFTPKHLPQDTGNVNFPSAWNWMADLKP
                     SVLRFPGGGSSRWMHLLPYKDEVAPFGVLDPIKGYGYDIKEIIRYYDVTDGSVEAHEL
                     TIMNSIISDMSDSTCDDCNSWMNDFKFQDDFENIYLKFLEQESNIPAHQQQQYIDQFI
                     ALIDTLQNQGDYTVDVIVDLNIISESATQCKKIIDYLQNPDPKIDGGNGVTSVHVVGV
                     EMGNECHLNWATDLMGFYAFDDYWNLINGIGKDDDPINELTEEFEVWLDDFSEYVFSE
                     EYYNDHDFIAAFKAEPTFNMKVGIPAANLKNNDSTKFAFRMMEDLSTSWNEDLVTHYH
                     DSLVIGGLTRYLFDAVILHTYYDSQTNWDTLATSNLCDSTYPNSGMPTCVTEDCDFWL
                     GDRWQFNTYDDRLKDAYEAVIGLGSNGFGNFKQFIRTRYAESYDQQRNDLLFAGTQSW
                     KKELWTTEWNMKDKNTDYVTGSMPQNILSSFCNSFEHGWITHEWFLNNIKQNYASGYR
                     NGFHTYSTFHAYGGGSFYAMLLQSDKADRINHLDSFGEPDTLNDPPSGQNLFLRKTVY
                     YTFEMLSEIQKKNLKYLQSNFTAYAHNPNVQPTVFIDKPNKKLYIYYSNMKPETQSYM
                     VKKGYVIGLYPGSTEFAYGEALLYNIDAQQLYSNSGNSYLYRMNICYNDSNQLHPFEI
                     QGINGPITNEPECTGLVDGAICITVPANSFGYIEIPFYVTTREGVILTEEQIELFPNP
                     TANTFRITCTIPEELFNEFVVDVFDLQGNLVKHTTTSQNSDIDVSNWPSTIYVIQISN
                     RVQSFSVTKKLIKIE"
     repeat_region   7610..8647
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=7610..7655
                     /rpt_unit_seq="gttgtgaattgatttcaaattatgttcttatttttggttcgac
                     aac"
     repeat_region   9135..9636
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9135..9180
                     /rpt_unit_seq="gttgtgaattgatttcaaattatgttcttatttttggttcgac
                     aac"
BASE COUNT         3174 a         1502 c         1823 g         3298 t
ORIGIN      
        1 ggggcatgta tttttaggaa aaataatagt taaaaatatt cataaatcga gccccgacca
       61 ccgcgtcggg gttttttatt gccccaaatt ttattattaa atttatttat tcatcacaaa
      121 ataaagtgat atgggaaagt tatctctaaa cgggaaagac cttagaaaaa taggttatcc
      181 tgaaagtaaa gttattggca ttgcaattca agtgatgcat caatattata aacatagtgc
      241 tgaagcagaa gctatcgata tattaaaaga tgtattggcc gcaccagatg cgtatttaga
      301 agatgctgca ttaggaaaaa ttgctgctgc attattagat gaaataagac caaaaaatga
      361 tgttattccg cttgagcaaa aaagaaagga atatgctatt tacggttttg aaaacattga
      421 aatgggcgcc attaatcaaa tggaagttgc catgaaatta cctgttactg ttgcaggggc
      481 attaatgcct gatgcgcatc agggatatgg tttgccaatt ggtggtgtgc ttgcaactaa
      541 caatgctgta attccttatg gtgttggtgt ggacattggc tgcagaatgt gtttgagtgt
      601 gtttgatatt ccgggtgatt ttattaacga caacgttgaa aaattaaaag gatttttaac
      661 cagaaatacc gtttttggcg ccggtgggac tttaaaaaat ccgatggatg atgctgtttt
      721 ggaaagaaaa gaattcagtg aaaataaatt actgaaatcg cttcacgata aagcatttaa
      781 acaaattggc acttcgggtt ccgggaatca ctttgtggaa tttggtatca ttgaaattaa
      841 acagtctacc gaaaaatttt atattcctcc tggtaattac ctgggattat tatcgcattc
      901 aggttcgaga ggccttggtg ctgcggtggc aaattattat accagattgg caagagaact
      961 atgcaaatta cctgatgtag cacaacacct ggcttatctc gatttaaata gcgcagaagg
     1021 tcaggaatat tgggcagcta tgaatttaag tggcgattac gcttcggctt gtcaccacca
     1081 aattcataaa aaaattgcca gaagtgttgg tgcacaggta ttaggtatgg ttgaaaatca
     1141 ccacaatttt gcctggaaag aaatgtacaa tgggcaagaa gtgatagtgc atcgaaaagg
     1201 agcaactcct gcaggaaaag gtgtattggg aattattccc ggttccatga ctgctcccgg
     1261 atacattgtt gagggtaacg gaattgcaaa ttcgattcaa tctgcttcac atggcgccgg
     1321 gagaaaaatg tcgcggactg ctgctaaaaa taatttcaac caaaaaatgt tggatgaaaa
     1381 attgaatgaa gcaggcgtgc atttaattgg tggcgacatt gatgaatcac cgatggccta
     1441 caaagacatc aacgaagtaa tgcaatatca aaacgaattg gtaaacatta ttggcatgtt
     1501 taaaccaaaa gtggttagaa tggcataata aaatatgttt tgttcaacac cctgaagtag
     1561 actaacgtca cttcagggct ttgccctaat ttatgaagga gagcaaggtc gccctgaagt
     1621 agactaaagt cacttcaggg gtattttaag aacaaaaaaa ttacttgtaa ttactcgatg
     1681 ccggagtctg caatggggca gactcctata attggtgttt cgggttattt atttgtgtgc
     1741 cacagggtta tgctgtttaa taggttacct attaatttat tgattaatta aatttttaac
     1801 atgaacattt tattcaacat agaagaaaaa atgatacgcg ttaataaata tttatatgaa
     1861 aacaaatgca taaaagccaa acgtctgctt gaggatattc ttgaagaaga acccgattgt
     1921 ggttctgctc attttgaaat gggcatgtta tattacaatg aattagcaga atacgacacg
     1981 gccctttatc attttgattt atgtaaacgt tttaaaccgg gatttattcc tgtatatcat
     2041 gtaaccgtgc aattgctgta tcagttaaat aaacacgaaa cattaataaa atatgccaac
     2101 tcgtgtttgc aaattgaagg agtaaataaa ttttttgtct ataaacacct cgctatgagc
     2161 tgtgaaacga ctttgttatt taaacctgcg attaaatact ataagcagtc aaaattgtat
     2221 gcgatacatg aatatgatat cacatttata aaaaacagtt taatacgcgt aaaagaaaaa
     2281 actgtaaaca aaagcaggaa ataataaaaa ggcaattttt taattgcctt ttttaattta
     2341 accagatgta atcgttgttt accctttcag caatttttaa tgcatgatcg tcggcaacca
     2401 ttttggctat tctaagtagt ttatcataat ccgggtcgcg ttttaaaata acatcacggc
     2461 tacccttggt aattaaatcg aggtcgtaga aaacacgtga tgaggttgca gacatggcca
     2521 ttgtaatagg tgctgcagaa cgtttagttg atttagatat caataaataa tccccttctg
     2581 ccaatggttt ccacttgccc caataatttc caaacaataa tttataatcg cggtattgat
     2641 ggttcaccaa gtcaacttct acaccatttt ttaaaaaaag gataacgata cctactaaaa
     2701 atatgcctac aaaaaacaag ttgccgtggc cggtaaacat aactatcaaa ctggtaaaaa
     2761 tagcagaaac accaagtggt gcaggaatta ccgcaccttt gtcggtttta aattttttta
     2821 attcgagatt gtcgagtggt tccataatat agggttttgt atgatatgac gaaacattta
     2881 tgtgaatatg ataaattaga ttaaaaataa tcgggtaaaa gcattttcta ttaatgaatt
     2941 cgtttttttt aatttaaatt gctgatttat cgtaactgaa tgaattaaaa tgtaaggact
     3001 ttaaagttca aattattaaa ccatttacac taaatagttc cgctttcagt cattgaaaat
     3061 tgatactttt taataacttt acgatgcaaa tatctccaac atgtcaataa atttaaaata
     3121 tctaatcaaa tcatcagacc ctgaagaaat ttacaatttt gtagctagtg ctaattttgc
     3181 cttgaaatta attaaagccg gcaagggtag taaagccaaa aggtatggaa atgcatactt
     3241 ttttgcaaag acaaatgaag atatggattc aagcgacttt gcaattgaaa ctggcaagct
     3301 aattaacgcg acaaagcatc aataaaatcc tcggcaaata gaaattgcgt aattcgcttc
     3361 tttgtttgca gtttgttaga ttagtctaat gaaatattaa attgccttta tttcttgtaa
     3421 tgcaatattt caccgacaaa gggttagagc aattcacaaa acttttaaat gattttattt
     3481 ttgtgtttat ttttgttcga caattgtata aacgcgaaag agacgccttt agaagtctct
     3541 ttcaccgagc agaacggatt atgataaatc acaaagcaca tcccgcaaca caaatataat
     3601 ataaaaataa ttaaagacgc gccattcaaa aaaataggaa gcattcttaa ggagattaag
     3661 cgcaattctc cctgtgctat attagccatt ccgaacgttt tttgaaaaga gctttaaacg
     3721 tcagattatg aattaatttc aaataatgtt cttattttta gttcgacaac taaacctatc
     3781 gttataacca atgtgttacg agtgcattca cgatattata actaaaaact tacctagacg
     3841 aatttcgttt gcttgtttaa ataagcgcaa acgacgatgt tttttcatat agtttataaa
     3901 aattattatt taacatttaa tatttttcgt atgaaaaaca atactacatt tactacacaa
     3961 aataaaattg gacttttgtc aattttaatc atctctttag cattagcaac cgcaagtagt
     4021 aacacaaatt cagtttttat tttaaataac aataaaattg aaaataaaat aaaaattaat
     4081 ccggatacac tccctgatgt aatcgataca ataaatagtt atgaacaggg cgacacaata
     4141 gcgcttgatt tggctggttt agatacgctt tttattttat ttgaagacac aacatttgcc
     4201 ggaagtgata cattaagcga caccattgcc cttcgattat ttgatgtaac taaagatatt
     4261 acaataacac cgggtgctat aaccaaaact gttgcacccg gactgtttgg cattcatatt
     4321 gaaggtattt ttacacctaa acatttaccg caagacactg gtaatgtaaa ttttccaagt
     4381 gcctggaatt ggatggctga tcttaaacct agtgtgctgc gttttcctgg tggaggtagc
     4441 agccggtgga tgcatctttt accttacaag gatgaagtag ctcctttcgg tgtgctcgac
     4501 ccaattaaag gatatgggta tgatattaaa gaaataatac ggtattatga tgttactgat
     4561 ggtagtgttg aagctcatga attaacaatc atgaattcta ttataagtga tatgagtgat
     4621 tctacatgtg atgactgcaa tagttggatg aatgatttta aatttcaaga tgattttgaa
     4681 aatatttacc taaaattttt ggaacaagaa agcaacatcc cggctcacca acagcagcaa
     4741 tacattgatc aatttatcgc gttaattgat acccttcaaa atcaagggga ttatacagtg
     4801 gatgtcattg tcgatttaaa tataattagt gaatcagcaa cacaatgtaa aaaaataata
     4861 gactatttac agaaccccga tcctaaaatt gatggcggta atggcgtaac cagtgtgcat
     4921 gttgttggtg ttgaaatggg taacgaatgc catctaaatt gggcgacaga tttaatgggt
     4981 ttttatgcgt ttgatgatta ttggaattta ataaacggga ttgggaaaga tgatgaccct
     5041 attaatgaat taactgagga atttgaagtt tggctagacg atttttctga atatgtattt
     5101 agcgaagaat attacaatga ccatgatttt attgcggcgt ttaaagctga accaacattt
     5161 aacatgaaag tgggcatccc ggcagcaaat ttaaaaaaca atgattctac aaaatttgct
     5221 tttcgaatga tggaagattt atccacttcg tggaatgaag atttagtaac ccattatcat
     5281 gatagtttag taattggagg acttacacgt tatttatttg atgctgttat tttacatact
     5341 tactatgatt cccaaacaaa ttgggataca ttagctactt caaatttatg tgattctacc
     5401 tatccaaatt ccggcatgcc aacctgcgta acggaagatt gtgatttttg gttaggagac
     5461 cgatggcaat ttaatactta tgatgaccga ttgaaggatg cttatgaagc agttattggt
     5521 ttaggctcaa atgggtttgg taactttaaa caattcattc gtacccgata tgctgaatct
     5581 tatgaccagc aaagaaatga tttattgttt gctggcacgc aatcctggaa aaaggaattg
     5641 tggacaacag aatggaatat gaaagataaa aatacagatt atgttacagg gtcaatgccc
     5701 caaaatattt tatcttcatt ttgcaattca ttcgaacatg gttggattac tcatgagtgg
     5761 tttttaaata atatcaaaca aaactatgct tcaggatatc gcaatggttt tcatacctat
     5821 tccacatttc atgcctatgg tggaggctca ttttatgcaa tgctactgca atctgacaaa
     5881 gcagacagga ttaatcattt agattcattt ggtgaacctg atactttaaa tgatccaccc
     5941 tccggccaaa atttattcct tagaaagaca gtttactata cttttgaaat gttgagtgaa
     6001 atacaaaaga aaaacttaaa atatttgcaa agtaatttta ctgcatatgc ccataatccc
     6061 aatgtacaac caactgtgtt tattgataaa cccaataaaa agttgtacat ttactattca
     6121 aatatgaaac ctgaaacgca aagttatatg gtaaaaaagg gctatgtaat tggcttgtat
     6181 cctggttcta cagagtttgc ttatggcgag gcattgttat ataatattga tgcacaacag
     6241 ttatattcca atagtggcaa cagttattta tatcggatga atatttgtta taacgatagt
     6301 aatcaattac atccttttga aattcaaggc atcaatggcc caattactaa cgaaccagaa
     6361 tgcacaggat tggttgatgg cgcaatatgt ataactgtgc cggcaaattc gtttgggtat
     6421 attgaaatcc ctttttatgt caccacacgc gaaggtgtaa ttttaacaga agaacaaatt
     6481 gaactttttc caaatcctac tgcaaataca tttcgaatta cttgcactat tcccgaggag
     6541 ttatttaatg aatttgtcgt tgatgtgttt gatttacagg gtaatttagt gaagcatacc
     6601 acaacaagcc aaaattcaga tattgatgtt agtaattggc cttctactat ctatgtaatc
     6661 caaattagta atcgcgtgca aagtttttcc gttacaaaaa agttgataaa aattgaatga
     6721 tggcagaaag aaaatttagt ttacaacaat actccagcgt tgccggggta ttgttaatca
     6781 tgcataataa tacacatgca caggctattt atacggacat agacccagat gttgttttac
     6841 aattagatgg caactacatc tatattgata tggacaatga tggctcaaac gatttctttt
     6901 tattaaaaga tagtcattat tttacaaatt ctgaatatag ttggtaccgt ttcaatagag
     6961 ttctatgggc aggccctaat ttcgacccgg aaaatgaaat tgcgggtgat tttgcaacta
     7021 atggcgcagg tggtgggaca acttatttcc catatgcatt attggaaggg aatataataa
     7081 acaatgattt aaattttaat tatggaactt atcaattaat gggaattggt ttttttgaga
     7141 ccgatgaaac accttgggac tggcattatt ttataggctc atggcaatat agaattgatt
     7201 ccgcctattt aggtgttcga tttttaggcg cagacgattg caaacactat ggctggataa
     7261 gatgttcagt aggtgactct ttaaaaacat taacggtgca tgactatgca tatgaattaa
     7321 aatgtaacac ccctattatt gccggcgata ctattggcga cacgactacg gttgaaatta
     7381 gtgaaataaa taatttggaa gcaataattt atactttcaa tcaaaacttg tatattaatt
     7441 tgcctgacca agacgaagta aatgtgtata tttttaatat gaatgggcag gaaatatata
     7501 aaacggtttc atctcaaaaa aaccttataa tcgacatggc aggtaaacca gttgggactt
     7561 atatagttaa actatcgaca aaggacaaac aattttccca aataattgag ttgtgaattg
     7621 atttcaaatt atgttcttat ttttggttcg acaacacggc aatggagatt gaatatgcca
     7681 atgcagttgt gaattgattt caaattatgt tcttattttt ggttcgacaa ccctgagctt
     7741 gattaaaaac aggcggctcg taagttgtga attgatttca aattatgttc ttatttttgg
     7801 ttcgacaact taactctcca gtagtaaaag atggagtggg ttgtgaattg atttcaaatt
     7861 atgttcttat ttttggttcg acaaccagta gatggcgcaa tagcaactcc ggcatagttg
     7921 tgaattgatt tcaaattatg ttcttatttt tggttcgaca acaaccaacc acagcgcatc
     7981 gtaatcttcg cagttgtgaa ttgatttcaa attatgttct tatttttggt tcgacaactg
     8041 cttctgattt tttgtgaagt ggtttggtgt tgtgaattga tttcaaatta tgttcttatt
     8101 tttggttcga caacaggttt aatcatccat tgcatcactt ctacgttgtg aattgatttc
     8161 aaattatgtt cttatttttg gttcgacaac ttcatcgacg cgcatataga gccgtcggta
     8221 gttgtgaatt gatttcaaat tatgttctta tttttggttc gacaaccccg gttgacaccg
     8281 ggcacgaagg ttacccgttg tgaattgatt tcaaattatg ttcttatttt tggttcgaca
     8341 acgtgtgcag ttgaaagaag ttaacaacaa aggttgtgaa ttgatttcaa attatgttct
     8401 tatttttggt tcgacaactt catcattaat gatgttatcg gacgtaaggt tgtgaattga
     8461 tttcaaatta tgttcttatt tttggttcga caacaaaatg tgaatgcggc cataccgttc
     8521 caatagttgt gaattgattt caaattatgt tcttattttt ggttcgacaa caattggtgg
     8581 gattatcgga attatttcac agttgtgaat tgatttcaaa ttatgttctt atttttggtt
     8641 cgacaacttc ggcaacaatt ggcacaacat tcttaaagtt gtgaattgat ttcaaattat
     8701 gttcttattt ttggttcgac aactaatgat ttcttacggg cagaaagtta tgagttgtga
     8761 attgatttca aattatgttc ttatttttgg ttcgacaacg gtgtcagatt cgatcagaca
     8821 tctgactatg ttgtgaattg atttcaaatt atgttcttat ttttggttcg acaacttcat
     8881 cattaatgat gttatcggac gtaaggttgt gaattgattt caaattatgt tcttattttt
     8941 ggttcgacaa caaaatgtga atgcggccat accgttccaa tagttgtgaa ttgatttcaa
     9001 attatgttct tatttttggt tcgacaacag atgttttgat gagaaccaac acagatgcgt
     9061 tgtgaattga tttcaaatta tgttcttatt tttggttcga caactatcac ttttgatatt
     9121 gctacaaaag ttaagttgtg aattgatttc aaattatgtt cttatttttg gttcgacaac
     9181 ttaccaacgg gcgttacatt ttcgggtagt gttgtgaatt gatttcaaat tatgttctta
     9241 tttttggttc gacaactatt cccatgcttc ttaaatatcg ctttgcgttg tgaattgatt
     9301 tcaaattatg ttcttatttt tggttcgaca accggccgga acgtcatcct tttacccgat
     9361 aagttgtgaa ttgatttcaa attatgttct tatttttggt tcgacaactt atggatggca
     9421 attttgccac ggtaatacgt tgtgaattga tttcaaatta tgttcttatt tttggttcga
     9481 caacatattt agatgaatta aatgatttat ttaagttgtg aattgatttc aaattatgtt
     9541 cttatttttg gttcgacaac ttgatggcca acacaggctt gaagcagcaa gttgtgaatt
     9601 gatttcaaat tatgttctta tttttggttc gacaactttt tctgatatca tttatgaaaa
     9661 gctgaagttg tgaattgatt tcaaattatg ttcttatttt tggttcgaca acttgaacgt
     9721 tacgggcaac catttgcaac cggttgtgaa ttgatttcaa attatgttct tatttttggt
     9781 tcgacaacac gccagtt
//
LOCUS       DAORKW010000071         9795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_160_length_9795_cov_4.204620, whole genome shotgun sequence.
ACCESSION   DAORKW010000071 DAORKW010000000
VERSION     DAORKW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160_length_9795_cov_4.204620"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..576)
                     /locus_tag="PLB46_04570"
     CDS             complement(184..576)
                     /locus_tag="PLB46_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75822.1"
                     /translation="MKRFSRILSVLFISFIGNSVIQAQVATDLELQLCHNWTISAIEV
                     MGAETEVPLLNANDKVNIHNDKTIELTIDGTNYAGTWTVNDAGTWMTIALPGNTGMRL
                     QLAEVTDTKLKVDHVNESAVHTIYIFTH"
     gene            741..4484
                     /locus_tag="PLB46_04575"
     CDS             741..4484
                     /locus_tag="PLB46_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75823.1"
                     /translation="MRLTLPGLLLCISPIASNSQSLVTAEIFHGNDAQSTASVTTDFD
                     NNMLCFTGFWDDLDADPGPGVLNFNSVGSQDIAITKLDPSGNLIWSKQIGGSGFAAAQ
                     VIKTDAAGNVIIFGYFNGTMDMNPGPGTSNLVSNGGDDVYCAKYNQDGNLVWATNIGG
                     TGTEQSYGFDLDAAGNPIVLGYFQNTVDFDPGVGTVNLTAGFSGSNFLLRLNADGSYN
                     NVIQMSSAYGNYLYVDNSDNIYITGLFWGTVDFDPGPAVFNLIAAGFSSDAFIVKLNS
                     AFVFQWAGIISGNSSEQGTTISVDETANAVIVAGFFEGTIDINPEPAVVNIATVDYVD
                     AFIVRLDGADGGFIWGKSIGGTGFQAIYGVGVNSLGSIWLAGNFSNTIDCDPGPGTAS
                     LTSGGSQDIMKVNWDADANYIDAEKIGGTNSDYASCLHIDNEGAVIISGIFEGIVDFD
                     PGDVTFNLNSAFTGWDGYVAKYCTVYTINNIINICEGESYFAEGANQTESGIYYDYYT
                     PIEGCDSIIITHLTVGNPTVDLGANYAICNGTTTTLNAGNPGATYLWNTGATTQTINV
                     STTGTYSVTVTDPSGCVGVDAITITVNPAPNVNLGADINACSGETILLNAGNPGATYL
                     WSTGATTQTINVTETGTYSVVVTNGFTCADNDVINVTFHPSPIVDLGSTIQLCEGETV
                     VLDAENAGATYLWNTGATTQTISVNTAATYSVVVTSAFGCDASDAVVVNVVANPVVDL
                     GDDLTVCSDAIITLDANNPGATYNWNTGATTQTIAVTESGTYDVIVTNGFGCTKSDFV
                     SITINPTPIIDLGTTIDFCENTTITLDAENTGSSYLWNTGAVTQTITTAIPGTYSVTV
                     TSDEGCIATDEVIINALPAPVINLGVDTGFCDGSDLLLDATTPDVSYLWNTGAETSSI
                     VIDEAGIYTVSITNDFGCVAADEIVIEVYVTPIVDLGDDGTYCLDEEIVLDASNPDCN
                     YVWNTGDTLAAINVTTSGIYNVLVTNANGCTAYDEISISFSPLPMASLGDDIISCSNE
                     PVILDATTPLCTYLWNTGATTATIEVSISGLYFVHITNSFGCSITDSIQVDFLPAPDV
                     NLSLPFTTICNNVAPVELTGGAPLGGSYFGEGVIDGLFSPAGLTPDTYEIHYVYVDAI
                     GCSDTATQTITVTICQNIQLSTLSEVLLFPNPALYSTTILNNSSTPITEFVIYDLHGH
                     KVLTSQLNLLPEQTYILNIADFPSGTYLIQYNNNQYQTLIIQH"
     gene            complement(4524..5024)
                     /locus_tag="PLB46_04580"
     CDS             complement(4524..5024)
                     /locus_tag="PLB46_04580"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HRF75824.1"
                     /translation="MNKQNVVFVLLLCVTQSLFAQEVLTLQKGEKPGLGKITELSWLA
                     GYYTGTGFGGNCDELWSPVLDNSMHGIFRYASNDSVQFSEYMIMEASEGTINLKLKHF
                     GRDLSPWEEKDIWTTFELIKIEGTIAYFDGITYAKNNDVLTIKLLLHEGDKSWVETFT
                     FNKTNF"
     gene            complement(5102..5845)
                     /locus_tag="PLB46_04585"
     CDS             complement(5102..5845)
                     /locus_tag="PLB46_04585"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRF75825.1"
                     /translation="MENNITNPATNYVMPRIGDTAPSFTAETTQGTIHFPEDYSGKWV
                     ILFSHPADFTPVCTTEFMTFAKMDKEFQSLNWQLIGLSVDSLYSHIAWLRTIKEKIEF
                     RDMKNIEVLFPLIDDIRMDVANKFGMIQPNESNTKAVRAVFFIDPSAKIRAIIYYPLS
                     TGRNFEEIKRVLIALQVADAYGVATPADWQPGEDVIVPPAGSCGVAKKRMDGQEEGVY
                     CIDWFFCMKSIDKEKIDDLFIRKKDLILN"
     gene            6194..6709
                     /locus_tag="PLB46_04590"
     CDS             6194..6709
                     /locus_tag="PLB46_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRF75826.1"
                     /translation="MSCTYTTTIKKFGTMGEKTGWTYIEIPSDIAEELNPGIKTSYRV
                     KGLLDVLRIQQIAILPMGDGNFIMPLNAAMRKALGKKSGYSITVKIAVDNSPFSLSDD
                     FMSCLADEPKALAYFNSLPGSHQRYFSKWIESAKTDSTKTKRITMAIRALAAKQGYGE
                     MIRSNKQNSSV"
     gene            6706..7101
                     /locus_tag="PLB46_04595"
     CDS             6706..7101
                     /locus_tag="PLB46_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75827.1"
                     /translation="MKKLFIVVSVILLSSCAVDEEINFTSSLCEAIATNDDRSLRTII
                     DDICDEYQPNVTINDPGGHQINLQKVIEDINNTFDCAEASLVCYACIETYPPISEIRI
                     ETTVDSVYYFVVLDILTPENNEMSFQDMH"
     gene            complement(7108..7956)
                     /locus_tag="PLB46_04600"
     CDS             complement(7108..7956)
                     /locus_tag="PLB46_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75828.1"
                     /translation="MEFHTRLFTMSKPVSVFFHVGLGKTASTYLQNKVFGKLKGLHYI
                     HPTKFRSWKKLAAQSSAEKVFLSREMDQQLEREVKKFAEVYPDTHAIIVLRRNDQWIA
                     SQYRRFLKNGYPYTFTEFFDVVQDKGFWKTSDAMFYPKIEILEKYFTSPPLVLFYDDL
                     RKEPFQFIDAIANYTGSTYDKSAISLDPHHTSYEEKQLKVMLKVSKKIFSSAPIYKEG
                     PRYKTWIRRRSRMLLCYIILYSALLIPSRFISKKPLIDPADLARVKQFYQEDWLRCEA
                     RRNKSL"
     gene            8026..>9795
                     /locus_tag="PLB46_04605"
     CDS             8026..>9795
                     /locus_tag="PLB46_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF75829.1"
                     /translation="MTSILHYSGYLRRNIMRRILTLFILLTGIIAVANAQTGTIRGFV
                     YDEENGEPVPFVNVIVANTDNIGAPSDINGFFSISNVPVGEQKLVMTYIGYDTLFYNV
                     LVEANKISNVKLNMKQSGVDLDIVTISSKTTAKLEEIQISTTTITPLQIKRLPSVGGE
                     PDLAQYLQVLPGVIFTGDQGGQLYIRGGSPIQNKVLLDGMVIYNPFHSIGLFSVFETD
                     IIRNVDVYTGGFSAEYGDRISAVVDITTKDGNKNQTQGEVSVSPFLARFLIEGPLKKS
                     TDKSSSLTYILTAKHSYLDKSSKVIYPYVNSQEGIIGNNGIPYSFTDLYGKVSLNSES
                     GNKLNLFGFNYRDSVNYSDVTTFKWNAIGAGTNFVIVPSESNMLIEGVFAYSNYTMVQ
                     NEADEKPRNSFVGGFNGGVDFTYYRPHGDVQYGVEVIGFETDYEFFNRLGVKYSQQQF
                     TTELGTYVKYRRSFNKKFVIEPSLRIQYYASLSEFSPEPRIGMKYNITDRVRIKFAGG
                     LYSQNLISTKSDRDVVNLFTGFLSGPDDDLYTIDGEEAKSKLQRSTHAIAGIEFDPSD
                     YIEVNVEPYIKYFNQLIEITRNKT"
BASE COUNT         3047 a         1728 c         1859 g         3161 t
ORIGIN      
        1 aaaacaaagg ccggtttgct ctcacaattg caggaattag cagaaaaaga aacggagaaa
       61 aacaccttta ataattttaa ttatttctac tggcttgaaa aggtacgcaa atggtaagtg
      121 caagtaaaaa gcaaaaaaaa accggcaaca tatattgctg ccggttaaaa aatataatgc
      181 ttattaatgt gtaaaaatat agatagtatg aactgctgac tcatttacgt gatccacttt
      241 caatttggta tctgtaactt cagccaattg caaacgcatt ccggtatttc ctggtaatgc
      301 aatggtcatc caagtacctg cgtcatttac tgtccacgta ccggcgtaat tagtaccatc
      361 aatagttaat tcaatagttt tatcattatg gatattaact ttgtcgtttg catttaatag
      421 cggcacttca gtttcagcac ccataacttc aatcgcgcta atggtccagt tatgacataa
      481 ttgcaattcc aaatcagttg caacttgtgc ttgtataacc gaatttccaa taaaagagat
      541 aaataataca ctcaaaatac gggagaatct tttcataaaa cagattttat tgatattaat
      601 taaaaataaa tgtttaacaa ttatactcaa tgtaaataag gctttatctg cattgatatg
      661 gtaaaaataa ggaaatttcc ccaaattagt ggcacaatgt ttggcaactt acccatgcca
      721 acacccaaaa atattcgcct atgcgcctaa cattacccgg ccttttatta tgtattagcc
      781 ctattgccag taactctcaa agccttgtta ctgctgaaat atttcatggt aatgacgcgc
      841 aatcaacagc atcagttaca acagattttg acaataacat gttgtgtttt accggatttt
      901 gggacgattt agatgccgac ccgggaccag gtgtgctcaa tttcaattcg gttggttcac
      961 aggatatagc catcaccaaa cttgaccctt cgggtaattt aatttggtcg aaacaaattg
     1021 gcggtagcgg ttttgctgca gctcaggtta ttaaaacgga tgctgccggt aacgtaatta
     1081 tattcgggta ttttaatggc accatggata tgaacccggg accgggaaca agcaacttgg
     1141 tgagtaatgg tggtgatgat gtgtattgcg caaaatataa tcaggatggc aatttagttt
     1201 gggcaactaa tattggggga acaggtactg aacaaagtta tgggttcgat ttagacgctg
     1261 cgggtaaccc aatagtatta ggttattttc aaaacaccgt agattttgat cccggggttg
     1321 ggacagtaaa tttgactgca ggtttttccg gttctaattt tttattgcga ttaaatgctg
     1381 atggcagtta taataatgtt attcaaatgt catcggcata tggcaattat ttatatgtag
     1441 ataatagtga taatatttat atcacaggat tattttgggg tactgtagat tttgatccgg
     1501 gtccggcagt ttttaattta attgctgcag gattttcatc cgatgcattt attgtaaaat
     1561 taaacagcgc atttgttttt cagtgggcag gaataatttc aggaaatagt tctgaacaag
     1621 gcacaacaat ttctgttgat gaaacagcaa atgccgttat tgttgccgga ttttttgaag
     1681 gaacaattga tatcaatccc gaacctgctg ttgtcaatat tgctacggta gattatgtag
     1741 atgcttttat tgtgcgactg gatggcgccg atggtggatt tatttggggt aaaagtattg
     1801 gaggaacagg ctttcaggcc atttatggag ttggtgtaaa ttcactcggc agcatatggc
     1861 ttgctggaaa tttcagcaat actattgact gtgatccggg tcctggaact gcttcactta
     1921 ccagtggtgg ctctcaggat attatgaaag taaattggga tgcagatgcc aattatattg
     1981 atgcagaaaa aattggtggt accaattccg attatgccag ttgtttacat atcgataacg
     2041 aaggtgccgt tattatttcc ggaatatttg aaggtatagt agattttgat ccgggtgatg
     2101 ttacatttaa tttaaattca gcttttaccg gatgggatgg ttatgtcgca aaatattgta
     2161 ctgtttatac tattaacaat atcatcaata tttgtgaagg tgaaagttat tttgccgaag
     2221 gcgctaatca aactgaatca ggaatttatt atgattatta tacacctatt gaaggttgtg
     2281 atagtattat cattactcac ttaacagttg gaaatcctac ggtagacctt ggtgcaaatt
     2341 atgccatttg taatggcact actaccacat taaatgccgg aaatccgggt gctacgtatt
     2401 tatggaatac cggtgctact actcaaacaa taaatgtaag tacaacaggc acttatagtg
     2461 taacagtaac tgacccttcg ggatgtgtag gtgttgatgc gataacaatt acggttaacc
     2521 cggcaccaaa cgtaaattta ggtgcggata tcaatgcttg cagcggagaa acaattttat
     2581 taaacgcagg taaccccggt gctacttatt tatggtccac tggtgctact acacaaacaa
     2641 taaatgtaac tgaaacgggt acttattctg ttgtggttac gaatggattt acctgtgccg
     2701 ataatgatgt gataaatgta acatttcatc cctcaccaat agttgattta ggaagtacga
     2761 tacaactttg tgaaggagaa accgttgtgt tggatgctga aaatgccggt gcaacttatt
     2821 tatggaatac cggtgcaact acacaaacca tttcagttaa tactgcagct acgtattcgg
     2881 tggtagttac ttctgcattt ggctgtgatg cttcagatgc agtagtggta aatgttgtag
     2941 ccaatcctgt tgttgattta ggcgatgatc ttactgtttg ttcggatgca ataataacat
     3001 tagatgccaa taatccggga gccacttata attggaatac gggtgcaaca acacaaacaa
     3061 ttgctgttac cgaaagtggt acgtatgatg taattgtaac taatggtttt ggttgcacaa
     3121 aaagtgattt cgtaagcatt accataaatc caactccgat tatagattta ggtacaacaa
     3181 tcgatttttg tgaaaataca actattactc tcgatgcaga aaatacaggc tcatcttatt
     3241 tatggaacac cggtgctgtc acacaaacta ttaccactgc tattcccggc acctatagtg
     3301 taaccgtaac ttccgatgaa ggatgtattg caacagatga agtaataata aatgcactgc
     3361 ctgcacctgt aattaactta ggtgttgaca ccggtttttg tgatggcagc gatttattat
     3421 tagatgccac aactccggat gtttcctatt tatggaacac cggagctgaa acatcttcca
     3481 tcgtaattga tgaagctgga atatacacag taagtattac caacgatttt ggttgtgtgg
     3541 ctgccgatga aattgttatt gaagtttatg ttacgccaat tgttgattta ggtgatgatg
     3601 gcacttattg tttggatgaa gaaattgttt tagatgcaag taatcccgat tgtaattatg
     3661 tatggaatac aggagataca ttagcagcaa ttaatgtaac aacatctggt atatataatg
     3721 tattagttac caatgcaaat ggttgcactg cttatgatga aatatccatt tctttttcac
     3781 cattgccaat ggcttcgttg ggtgatgata tcataagctg ctccaatgaa cctgtaattt
     3841 tagatgccac tacgccattg tgcacatatc tctggaacac aggtgcaaca actgcaacaa
     3901 tagaggtaag tatttcaggt ttgtattttg tacatattac aaattcattt ggttgctcta
     3961 ttacagattc catacaggta gattttttac ctgctcctga tgtcaattta tcgttaccgt
     4021 ttacaacaat atgtaacaat gttgcaccgg tagaattgac cggaggcgca ccattaggag
     4081 gtagttattt tggcgaaggg gtaattgatg gattattttc acctgcaggt ctcacaccgg
     4141 atacttacga aattcattat gtttatgttg atgcaattgg atgcagtgat actgctacgc
     4201 aaacaattac agttactatt tgtcaaaaca ttcaattatc aacgctgagt gaggttcttt
     4261 tattcccaaa tcctgctttg tattctacta caatattaaa taatagcagc acacccataa
     4321 ctgaatttgt tatttatgat ttacatgggc ataaggtttt aacttcgcaa ttaaacttat
     4381 tacctgaaca aacgtatata ttaaatatag cagatttccc ttcaggaacc tatcttattc
     4441 aatataacaa taatcaatat cagacactga taatacaaca ttaaattagt gctgtcactt
     4501 gtaaaattga ccccactaat tatttagaaa tttgttttat taaacgtaaa ggtttccacc
     4561 caacttttat ctccctcatg caacaatagt ttaatcgtta acacatcgtt gttttttgca
     4621 taggtaatgc catcaaaata agcaatggtg ccttctattt taattaattc aaaggtggtc
     4681 catatatctt tttcttccca aggagaaagg tctctcccaa aatgttttaa tttgagattg
     4741 atggtgcctt ccgatgcttc cattatcatg tattcggaaa attgaacgga gtcgttgctg
     4801 gcatatcgga aaatgccatg catcgaatta tctagtaccg gcgaccataa ttcatcgcaa
     4861 ttgccgccaa atccggttcc ggtatagtat cctgctaacc aactgagttc tgtaattttt
     4921 cccaatcccg gtttttcgcc cttttgcaat gtgagcactt cttgtgcaaa taacgattgt
     4981 gttacacata acaacaatac aaaaactaca ttttgcttat tcatatgcct gatttattaa
     5041 ataaagataa aaaaaaggcc gtcaatcaga cggccctcac atataaatct aagcttcaaa
     5101 cttaatttaa aatcaggtct tttttacgaa taaacaaatc atcaattttt tccttatcaa
     5161 ttgatttcat acaaaagaac cagtcgatac aatacacacc ctcttcttgg ccgtccatac
     5221 gttttttagc tactccacat gaaccagcag gtggaacaat aacatcttca ccgggttgcc
     5281 agtcggcagg agttgctaca ccataagcat cagcaacctg caaagcaatt aatactcttt
     5341 taatttcttc aaaattgcga cctgtagaaa gcggataata tataatagct ctaatttttg
     5401 cagaagggtc gataaaaaat acagctctta ctgctttcgt attactttca ttgggttgta
     5461 tcattccaaa tttattggca acatccattc ggatatcatc aataagcggg aataacactt
     5521 cgatattttt catatcacga aattcgattt tttctttaat cgttctcaac caggcaatat
     5581 gactgtataa actatctact gataaaccga tgagctgcca attcagcgat tgaaattcct
     5641 tatccatttt cgcaaacgtc ataaattcag tagtacatac cggtgtaaaa tccgcagggt
     5701 gactaaataa aatcacccat tttccggaat aatcctccgg gaaatgaata gtgccctgag
     5761 ttgtttctgc tgtaaacgaa ggggctgtgt cgccaatacg cggcataaca tagtttgttg
     5821 ccggatttgt tatattgttt tccataatta cttattattt caatgatagt acaaagttgc
     5881 gatgggttaa ccgtgaattg tgtgatttat gtgacataag cacctgattt ttgtcaactt
     5941 cgaaaaattt ggataggcct ttgtaatcca taaattatgc aaaaagcaac agttgcaata
     6001 ggttgctaac tttctaggaa tgtggttatg aaattatttg ttaatgcgtt gattgacatc
     6061 gtattagtag ttattgatta aacttaacca caactattaa gcctttaagc atttggtctg
     6121 ataaatgcca ttttttgcga ttttaacaat tcaaaattac cattcgaaga agattttatt
     6181 atatttgaat ttgatgtcgt gcacctatac tactactata aaaaaattcg gcaccatggg
     6241 tgaaaaaacc ggatggacct atatagaaat cccatctgat attgctgagg agttaaatcc
     6301 cggtataaaa acatcctaca gagtgaaggg attattagat gttttacgta ttcaacaaat
     6361 tgctatttta cctatggggg acggcaattt tattatgcct ttaaatgctg ccatgcgcaa
     6421 agcacttgga aaaaaaagcg ggtatagtat aacagtaaaa attgcggttg ataatagtcc
     6481 gttttcactt tctgatgact tcatgtcttg tttagcagat gaaccaaaag ccctggctta
     6541 ttttaattca ttgccgggtt cacatcagcg gtatttttca aagtggatag agagtgcaaa
     6601 aactgactct accaaaacaa aacgtataac catggctatt cgtgcacttg ctgctaaaca
     6661 gggttatggt gaaatgattc gttcaaataa acaaaattca agcgtatgaa aaaattattt
     6721 attgtagtta gtgttatttt attaagcagc tgcgcagttg acgaggaaat caattttacc
     6781 agttctttgt gtgaagcaat tgcgacaaat gacgacagga gtttgcggac aattattgat
     6841 gatatttgcg atgaatacca accaaatgta acaataaatg acccgggcgg acatcaaata
     6901 aatttgcaaa aagtaattga agatattaat aacacatttg attgtgccga ggcttcattg
     6961 gtgtgttacg cctgcattga aacctatcca cccatttctg aaatacgtat agaaactact
     7021 gtagacagcg tttattattt tgtagtactt gatattctta ctcctgaaaa caatgaaatg
     7081 tcgtttcagg acatgcatta attttgatta caaggattta tttctcctgg cttcacaacg
     7141 caaccagtct tcttgataaa attgttttac acgcgctaaa tctgccgggt ctatcaacgg
     7201 ctttttgctg ataaatctgc tgggaattaa taatgcgcta tataaaataa tataacacaa
     7261 caacatacgt gaacggcggc gaatccatgt tttataacgc gggccttctt tataaatagg
     7321 tgctgatgaa aatatttttt tgcttacttt caacattacc ttgagttgtt tctcctcgta
     7381 agaagtgtgg tgaggatcta aagaaatagc tgacttatcg tatgtgctgc cggtataatt
     7441 ggcaatagca tcaataaact gaaagggttc ttttcttaaa tcatcataaa ataataccag
     7501 cggtggggat gtaaaatatt tttccagaat ttcaattttc gggtaaaaca tggcatcact
     7561 tgttttccaa aatcctttgt cctgcaccac atcaaaaaac tcagtaaacg tatacggata
     7621 accatttttt aaaaacctgc ggtattgtga tgcaatccat tggtcgttgc gacgcaaaac
     7681 gataatggca tgcgtatccg gataaacctc ggcaaacttt ttcacctccc tttccagttg
     7741 ctggtccatt tcgcgggaca aaaacacttt ttcggcacta ctttgtgcgg ccagtttttt
     7801 ccaagagcgg aattttgttg gatggatata atgtaagcct ttgagcttcc caaacacctt
     7861 gttttgaagg taggtactgg ccgttttacc cagcccgaca tgaaaaaata cacttaccgg
     7921 tttgctcatc gtgaataatc gcgtgtgaaa ttccataaaa atatgttgtg gtgcaaatac
     7981 ttacgaaatg ttaatgaagg tcaaaacact gtaaaattgg gtgttatgac atctatttta
     8041 cattattctg gttatcttcg ccgcaatatt atgcgcagaa ttttaacctt atttatcctc
     8101 cttacaggga ttatcgctgt ggcgaacgca caaaccggaa caatccgtgg gtttgtgtat
     8161 gatgaagaaa acggagaacc ggtgccattt gtcaacgtta ttgtagccaa taccgataat
     8221 attggtgccc cttccgacat caacgggttc ttctctattt ctaatgttcc ggttggcgaa
     8281 caaaagttag tgatgaccta tatcggttac gataccctat tttacaatgt tttagttgag
     8341 gcgaataaaa tttccaatgt taaactgaat atgaaacaaa gcggagtaga tttggatatc
     8401 gttaccattt cgtccaaaac caccgccaaa ctggaagaaa ttcagatttc cacaacaacg
     8461 attacacctt tgcagataaa acgattacca tcagtaggtg gtgaaccgga tttagcacaa
     8521 tatttacagg tgcttcccgg ggttattttt acaggcgacc agggtggaca gttatacatc
     8581 agaggtggta gccctattca aaataaagtt ttgttagacg ggatggtaat ttacaatccc
     8641 ttccactcta ttggtttatt ttcagttttc gaaacagata tcatcagaaa cgttgatgtt
     8701 tacacgggag gatttagtgc tgaatatggt gatagaattt ctgctgttgt agatattact
     8761 accaaagacg gtaacaaaaa tcaaacccag ggtgaagttt cggttagtcc tttcctcgct
     8821 cgtttcctga ttgaaggtcc attaaaaaaa tctaccgata aatcatccag tttaacgtat
     8881 atattaactg ccaaacattc ttatttagat aaatcttcaa aagtcattta tccttatgta
     8941 aacagtcagg aaggaattat tggcaacaat ggtatacctt attcttttac cgatttatat
     9001 ggtaaagttt ctttgaattc agagtccggc aataaattaa atttatttgg atttaattat
     9061 agagatagtg ttaattattc agatgtaact acatttaaat ggaatgcaat tggcgcagga
     9121 acaaattttg ttattgttcc atctgaatcc aatatgttga ttgaaggcgt atttgcttat
     9181 tctaattata ccatggttca aaatgaagct gatgaaaaac cgcgtaacag ttttgtgggt
     9241 ggatttaatg gcggggttga ctttacctat tatcgtcctc atggcgatgt gcaatatggt
     9301 gtagaggtta ttggctttga aactgattat gaatttttta atcgtttagg tgttaaatat
     9361 tcacaacaac aatttactac cgaattaggt acttatgtaa aatacagaag gtcgtttaat
     9421 aaaaaattcg ttattgaacc aagtttacgc attcagtatt atgcctcatt aagtgaattt
     9481 tctccggaac cgcgaattgg tatgaaatac aatattaccg atagagtgcg tataaaattt
     9541 gcaggtggtt tatatagcca gaatcttatt tcaaccaaaa gcgatagaga tgtagtgaat
     9601 ttattcaccg gatttttatc aggtcctgat gatgatttat ataccattga tggcgaagaa
     9661 gcgaagtcga aattacaacg ttcaacacat gccattgccg gtattgaatt tgacccttct
     9721 gattatatag aagtgaatgt agaaccttat attaaatatt ttaatcaatt aattgagatc
     9781 accagaaata aaacc
//
LOCUS       DAORKW010000072         9718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_164_length_9718_cov_4.833489, whole genome shotgun sequence.
ACCESSION   DAORKW010000072 DAORKW010000000
VERSION     DAORKW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9718
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164_length_9718_cov_4.833489"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="PLB46_04610"
     CDS             <1..1345
                     /locus_tag="PLB46_04610"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF75830.1"
                     /translation="KLFTPFLIIAAMLFTLASCTNMSGKDAAPNAVNAIPKLLDNPLT
                     TGSDVEWETILTSYDKAIATLNQNPAALDQYIVLAQVFITEARLTGNSGYYNEAAIKM
                     LDKVLTDENKNDELVFQALTLKSGVLLSMHQFNDALATANTAFAISNHNAQLLGALVD
                     AHVELGNYTEAVKYCDMMMQLRPDIRSYSRVSYLRQIHGDNAGAIEAMKMAVESGLPG
                     AESTEWARVVLGDLLIMIGDLKNAEICYTTADGLRNNYAYAKAGLGRLAKAKGDYNAA
                     ITYTEQAIAIISDVAFVNQLGDLYALKGDQQKAGEIRADVMDLLKDAEKEQNKNGAAI
                     KHNGARELAMAYMQNGKMADAYTFAKKDLATRQSNIDANELAAWTAYLNKDYTQAKIY
                     AEKMLATNTKNPVTLYKAGIILKKNGDNEKGTTLINEATAMQPNIANQVNTAITY"
     gene            1396..2052
                     /locus_tag="PLB46_04615"
     CDS             1396..2052
                     /locus_tag="PLB46_04615"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HRF75831.1"
                     /translation="MKKITFLSLFVLIAFQQSYAHTINYTLDKMNDGEVFWQYLREGF
                     LHIIPLGLDHILFILCVFFLNTNLKKIILQASMFTLAHTITLGLSMYGIINPPSGIVE
                     PIIALSIVFLAVENIYAQKVKPWRLIMVFIFGLVHGMGFAGALNDLGMPAYAFSTALI
                     SFNIGVELGQLAIIFAMYFLVSKQFSNAVWYRSKLVIPVCMIIAVIAGYWTLERFLTL
                     "
     gene            2087..2509
                     /locus_tag="PLB46_04620"
     CDS             2087..2509
                     /locus_tag="PLB46_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75832.1"
                     /translation="MQAALQYQHKQESVSVMLLLIVVLTLLRPITPFITDSIAHHFYS
                     QQHEQLHRIGINHLEKDLQDANETQSSSEKKGNGFEQILIFFLQFVLSPAIAIFSIIL
                     FKEPKCWQLRTSKKPTLQPPDANFVVCPDNTTPSVKVV"
     gene            2617..4893
                     /locus_tag="PLB46_04625"
     CDS             2617..4893
                     /locus_tag="PLB46_04625"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF75833.1"
                     /translation="MNTFCKKYNNYLVILLLTFSQSVFAHNGSIQGNITQQSNGQPVS
                     DAMIFVSELSTTTFTNTFGNYTITDLPKGTYHLSIVHIGFEQLTAELTVTDATTSTAN
                     FVLTEVPISLEQVVVRSTQTDQMRAVNVLDMQLRPTENAQDLLRLVPGLFIAQHAGGG
                     KAEQIFLRGFDIDHGTDISVNVDGIPVNMVSHAHGQGYADLHFLIPETVQSFDFLKGP
                     YYAGYGDFATAAAVNFKTKNTLDYNTIKVSAGQFNTLRTLLMMQLPIAEKTASKSSAY
                     LAGEYQITDGPFNSPQDFTRVNLFGKYHTSMRNNTILQLSASTFSSRWNASGQIPQRA
                     IDMGLIDRFGAIDDREGGVTGRSNINVELIKSINTNTTISNQIYFSDYQFELYSNFTF
                     FLEDSINGDMIKQKEHRHLYGYKSKLSHESIVAGKLLTTETGIGFRYDKSMENELSHV
                     AEREFLLNRLAFGNIYQTNAFIYLDETLQLTDRLAVNASLRYDMFNFAYDNLMDSSYQ
                     FKSVDDGILSYKLNFNYTVNNNLGVYLHTGKGFHSNDSRVVVAQNALQTLPAAYGADL
                     GLQVKPYRNLILNPAVWILYLEQEFVYVGDAAVVEAGGKTLRRGFDLSARWELTDWLF
                     VDADINYTLAELIGSPEGANYIPLAADLTAIGGLNIRTENGWSGSLRYRYMDDRPANE
                     TGSVIAEGYFVNDAVINYTTKNYALGIEVQNLFNVDWNEAQFDTESRLLDEPTPVSEL
                     HFTPGTPFFAKGSVSLFF"
     gene            complement(4928..5266)
                     /locus_tag="PLB46_04630"
     CDS             complement(4928..5266)
                     /locus_tag="PLB46_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75834.1"
                     /translation="MIITVTYIHLRKPWFFFRLSYFGYKIMRQAQNSSGFIRMSNSGF
                     WRDHYTLSAWENEAAMKNFARSGEHLKAMKQSQSISDKLATYSYVAEKFPDWNTAKQL
                     IHKNGKVLTF"
     gene            5428..6459
                     /locus_tag="PLB46_04635"
     CDS             5428..6459
                     /locus_tag="PLB46_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF75835.1"
                     /translation="MIQALGSLPKGAVLERIRQAPNYTSNGFENLSHTPTMAEGHNFF
                     KVMRAFMNKPKGSVPTSIIPSIQNDLNRLPDGNPAMVWFGHSSYLLKVEGKNILVDPV
                     FSGNASPFSFMVKAFNGSNVYSAENMPDIDILIITHDHYDHLDYQTIKALKSKVKQVV
                     CSLGVAAHLIKWGYDAKIITELYWGEKTTIQNIQFTAAPARHFSGRTIIRNRSLWSSF
                     ILRTVSHQIYIGGDSGYDTHFKQIGEDFGKFDLAILECGQYNPAWKYIHMMPEETVQA
                     AIDLNADIFIPVHWGKFDLSLHHWTEPIERATAHAQKLNMRYFTPMIGAISQLNKEMP
                     THNWWRDVK"
     gene            complement(6575..7249)
                     /locus_tag="PLB46_04640"
     CDS             complement(6575..7249)
                     /locus_tag="PLB46_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75836.1"
                     /translation="MKIITLFGVFMVLSACSKEQKKDISMTDYLTAGIPSNGYRTYMH
                     NSDSLPITVLLKQNVQIINDTTLTISTYWLNSDSIPMNFAVEKWFVNNNIQLLKQSLF
                     EPIGPGKVVELKGNFDTTKICSFFDNNLKLSISFKGIADTSYLMTINASGKAEIDSIK
                     DLDGQWVSGLIISNTEQTTFDFIDNRTDTTTSSTLYRYYTLDQGLIYFKAISGSDTSQ
                     FFLTKN"
     gene            7440..8552
                     /locus_tag="PLB46_04645"
     CDS             7440..8552
                     /locus_tag="PLB46_04645"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HRF75837.1"
                     /translation="MQQKQSVKFINRDKSLFYQTVKENVNKYFTDNHISKFGNNTMYM
                     KSFVLLTAYIGAFSLILIFNPPFIVSMLLWFTMGITVAGVGMSVMHDANHGAYADNKY
                     VNMMMGYTLNLLGGTVHNWKLQHNILHHTYTNITGLDDDIDDKAGMRFSPHNTWKPKH
                     NFQYVYAFFLYGLITLYWCIAKDFAQFSKYTANGVNANSRSANIIMFIKLIFLKAGYF
                     FLFLVMPTMFFEIPFIQVLTGFLIMHFVGGMILSVVFQLAHTVEGTSHPMPSTDGTIE
                     NCWAIHQLNTTMNFSRKNKWLSWYVGGLNFQVEHHLFPKICHVHYPEIAPIVKATAEQ
                     FNIPYLESKTFGSALQSHIKTLKRLGKMPVLDEIMG"
     gene            complement(8555..>9718)
                     /locus_tag="PLB46_04650"
     CDS             complement(8555..>9718)
                     /locus_tag="PLB46_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75838.1"
                     /translation="GARLTGKANGWQMGALNMQTLGIADEGISPHNFTVLRTRKDIDS
                     LGSFVGGIITNKINTDTSNTSNQSVGLDFVKRFTQQFTVEGGVASTLINLQANDLIDA
                     SYLHLGVFKSAVTGFTYSSTLDFLGNEIRPDMGFIDDNNYGNSRTQLTYRVGLPESSK
                     LQYMYLNSTNNYRWRFDTHKRETHAIDLWPGVQFKNGMEINVSLFEYKIDSLPFDWIL
                     DENNAIAAGTYTTWNNSLEIYSPAQSALRLALIGVYGGFYSGQKLTLMPNINYYINAH
                     LNFSLYYEFNNINFDTYLTDTVSTQFVSNLIRWNVTYNFTTKVSLRFYVQYDDLSDLV
                     SANLRFRYNPKEGTDLFIVYNQGTNTDVTRLDPHLPVVDNQAVTVKFIKTFEL"
BASE COUNT         3104 a         1633 c         1858 g         3123 t
ORIGIN      
        1 aaagctattt acaccctttc ttattatcgc agctatgctc tttacactcg cttcatgtac
       61 taatatgagt ggtaaggatg ctgcacctaa tgcagttaat gcaattccta aattactgga
      121 caatccatta acaaccggtt ccgatgtgga atgggaaaca atattaacca gttacgataa
      181 ggcaatagcc acattgaatc aaaatccagc agcgttggac caatatattg ttctggctca
      241 ggtatttatc acagaagctc gtttaacggg caattccggt tattacaacg aagcggctat
      301 aaaaatgctg gacaaggttt tgacagatga aaataaaaat gatgaattgg tattccaggc
      361 acttacctta aaatcaggtg tgttattatc gatgcatcag tttaatgatg cgctggcaac
      421 tgccaatacg gcttttgcca tcagtaacca taatgcacaa ttattaggtg ctttagtaga
      481 tgctcatgta gaattgggaa attatactga agcagttaaa tattgtgata tgatgatgca
      541 attacgtccc gatattcgtt cttattcacg ggtgagttat ttacgtcaaa ttcatggcga
      601 taatgcgggt gcaatagagg caatgaaaat ggccgttgaa tccggattac ctggagcgga
      661 atctactgaa tgggcaagag tagtgttggg cgatttatta attatgatag gcgatttaaa
      721 aaatgcagaa atatgttata ctactgcaga cggtttgcgc aataattatg cttacgcaaa
      781 agcaggtttg ggaagattag caaaagctaa aggtgattac aatgctgcca ttacttacac
      841 tgaacaggcg atagcaatta taagtgatgt tgcatttgtc aatcaacttg gtgatttata
      901 cgcattaaaa ggtgatcaac aaaaagccgg cgaaataaga gctgatgtga tggacctgct
      961 aaaagatgca gaaaaagaac aaaataaaaa tggtgcagca attaagcaca atggcgctcg
     1021 cgaattagca atggcttata tgcaaaatgg gaaaatggca gatgcttata catttgctaa
     1081 aaaagattta gcgacaagac aatctaatat tgatgctaat gaattagcgg cttggactgc
     1141 ctatttaaat aaagattata cacaggcgaa aatatatgcc gaaaaaatgt tggcaaccaa
     1201 cactaaaaac cctgttacct tgtataaagc cggtattatt cttaaaaaga acggcgacaa
     1261 tgaaaaagga actaccttaa ttaatgaggc tactgcaatg cagccaaata ttgccaatca
     1321 ggtaaatact gctattactt attaatattc caaccataac cttcctcttt tgaggaaggt
     1381 tttaattaag taatgatgaa aaaaataaca tttttaagcc tatttgtgtt gatcgcattt
     1441 caacaaagtt atgcgcacac cattaattac accctagata aaatgaatga tggagaggtg
     1501 ttctggcaat atttacgaga agggtttttg catattattc cccttggatt ggatcatatt
     1561 ttatttatcc tttgcgtgtt ttttctaaat accaacctaa aaaaaataat attacaggca
     1621 agtatgttta cgcttgcaca taccattacg cttggattga gtatgtatgg tattattaat
     1681 ccaccaagtg gaattgttga acctattatt gcactttcta ttgtatttct tgcagtagaa
     1741 aacatttacg cacaaaaagt aaaaccctgg cgactcatca tggtatttat ttttgggtta
     1801 gtacatggca tgggattcgc cggtgcctta aatgatttgg gaatgccagc atatgcattc
     1861 agcacagcat taataagttt taatatcggt gttgaactcg gtcagttagc aatcattttt
     1921 gcaatgtatt ttttggtgag taaacaattt agtaatgcag tatggtatag aagtaagttg
     1981 gtgattccgg tttgtatgat tatagccgta atagccggtt actggacatt agagcgtttt
     2041 ttaacattat aaaatccatt gaccaagttt ttacgtaaat ggtattatgc aagcagcact
     2101 tcaatatcaa cataaacagg aatccgtgtc agtgatgtta ttactgatag tggtgttgac
     2161 attgctgcga cctattacac catttattac cgattccatt gcgcatcatt tttattctca
     2221 gcaacatgaa caattgcacc gaattgggat taatcacctg gaaaaggatt tacaagatgc
     2281 taacgaaaca caaagttcat cagaaaaaaa aggaaatgga tttgagcaga tattaatttt
     2341 ctttttgcaa ttcgttttat ctcccgcaat agcaattttc tctataatac tatttaagga
     2401 acctaaatgt tggcaactac gtacatctaa aaagccaacg ttacaacctc cggatgcaaa
     2461 ctttgtagtc tgcccggaca acacaacacc aagtgtaaag gttgtttaag gttagcaaaa
     2521 ttaaaggcag actatcttct tcatcattta gcatacaggc ggcagtgtgg ctgcactgcc
     2581 gcttttttat tgcctttact ttgtaaaacc tcaattatga atactttttg taaaaaatac
     2641 aataactatc ttgttatatt attactcaca ttttcacagt ctgtttttgc gcacaatggc
     2701 agcattcagg gaaatatcac acagcaatct aatggacaac cagtttcaga tgccatgatt
     2761 ttcgtgagtg aactatcaac tactacattt accaatacct ttggaaatta tactattact
     2821 gatttaccta aaggaactta tcatttatcg attgtacaca tcggtttcga gcagttgact
     2881 gcagaactaa cggtgacaga tgcaaccact tctaccgcaa atttcgtgtt gaccgaagtg
     2941 cctatttcgc ttgaacaagt tgtagttcga tcgacgcaaa ctgaccaaat gcgcgctgta
     3001 aatgtgttgg atatgcagtt acgaccaact gaaaatgcgc aggacttatt gcgtttagtg
     3061 cccggattat ttattgctca gcatgcagga ggcggaaaag ccgagcaaat atttttaaga
     3121 ggatttgata ttgaccatgg aacagatatc agtgtaaatg ttgatggaat tccagtaaac
     3181 atggtaagtc atgcacacgg acagggctat gcagatttac attttttaat accggaaact
     3241 gtgcagtcct ttgacttttt gaaaggccct tattatgccg gttatggcga ttttgccaca
     3301 gctgcagccg taaattttaa aacaaaaaac acgctggatt ataataccat taaagtgagt
     3361 gcagggcaat ttaatacctt gcgcacctta ttaatgatgc aattaccaat agccgaaaaa
     3421 acggcgtcaa aatcatctgc ctatttggcc ggcgaatacc aaattaccga tggtccgttt
     3481 aatagccctc aggattttac cagggtgaat ctttttggta aatatcatac ttccatgcgc
     3541 aataatacta ttttgcaatt aagtgcatca acatttagta gtagatggaa tgcatcaggc
     3601 caaataccgc agcgtgcaat tgatatgggt ttgatagata gatttggtgc aattgatgat
     3661 agggaaggag gtgttacagg cagatcaaat ataaatgtag aattaattaa aagtataaat
     3721 acaaatacca ctatttctaa tcaaatttat tttagtgatt atcaatttga attatatagc
     3781 aattttacat tctttttgga agacagtatt aatggtgata tgattaaaca aaaagaacac
     3841 cgacatttat atgggtataa atcgaaatta tctcatgaat ctattgttgc aggtaaattg
     3901 ttgactacag aaaccggaat tggattcagg tatgataaaa gtatggagaa tgaattgtcg
     3961 catgtggcgg aacgcgagtt cttattgaat cgtttggcat ttggtaatat ttatcaaacg
     4021 aatgctttta tttatttgga tgaaacattg caacttacag acaggcttgc tgttaatgcc
     4081 tcgttgcgat atgatatgtt caattttgca tatgacaact taatggatag tagttatcaa
     4141 ttcaagagtg ttgatgatgg cattttatcc tataaattaa atttcaatta cacagttaat
     4201 aacaatttag gcgtttattt acacacaggt aaaggcttcc attcaaacga tagcagggta
     4261 gtagtcgcgc aaaatgcact gcaaacgtta cctgctgctt atggtgctga tttaggctta
     4321 caggtaaaac cctaccggaa tttaattttg aatcctgcag tgtggatatt gtacttagaa
     4381 caggaatttg tatatgttgg cgatgcggca gtagtggaag caggtgggaa aacattacgc
     4441 agaggatttg acttatcagc tcgatgggaa ttaactgatt ggttatttgt tgacgctgat
     4501 attaattaca ctttagcaga attaattggt tcccctgaag gagctaatta tattcctctt
     4561 gcagcagatt taacagcaat tggcggattg aatattcgaa ctgaaaatgg atggagtggc
     4621 agtttacgct accgctacat ggatgatcgt ccggcaaatg aaacagggtc ggtaattgca
     4681 gaaggttatt ttgtaaacga tgcggtaatc aattatacaa caaaaaatta tgcgctggga
     4741 attgaagtgc aaaatttatt taatgtagat tggaatgaag cacaatttga tacagagagt
     4801 cgtttgttgg atgagccaac accggtgagt gaattacatt ttactccagg cactccattt
     4861 tttgcaaagg gaagtgtaag cttgtttttt taattaatag gaaaggtgct gcattttgtt
     4921 aataaattta gaatgtcagc acttttccat ttttatggat taattgttta gctgtattcc
     4981 aatccggaaa tttttctgca acgtatgaat atgttgccaa tttgtcagaa atagattggg
     5041 attgtttcat tgcttttaaa tgctcaccag aacgtgcaaa atttttcatg gctgcttcat
     5101 tttcccatgc actcagggta taatggtcgc gccaaaaccc actgttcgac atacgaatga
     5161 acccactgga gttttgtgcc tgacgcataa tcttatagcc aaaataactc aacctgaaaa
     5221 agaaccaagg tttacgcaaa tgaatatagg tgacagtaat aatcatacaa agagttttgc
     5281 ctagtcaaag ataggtggta taagcgatta tataaaaaaa ttgttttaaa cctacgctca
     5341 tgtggttctg aacattagcg gcaataaaat gtttaatggc ttacctatct ttcacaatat
     5401 ttgaattttt aacacgtcat caccattatg attcaggctt taggcagttt accaaaagga
     5461 gcagtccttg aaagaatacg acaagcaccc aattatacct caaacggatt tgaaaattta
     5521 tctcatacgc ctacaatggc tgaggggcat aactttttta aagttatgcg ggcatttatg
     5581 aataagccga aaggaagcgt tccaacttca attattccat ccatacaaaa cgatttaaat
     5641 cggctgcctg atggcaatcc tgctatggtt tggtttgggc actcttcata tttattaaaa
     5701 gtggaaggca aaaatatttt agtagaccct gtatttagcg gtaatgcctc accattttca
     5761 tttatggtaa aggctttcaa tgggagtaat gtgtatagtg ctgaaaatat gcccgatatt
     5821 gatattttaa ttattacaca cgatcattat gaccatttgg attatcaaac tattaaagca
     5881 ttaaagtcga aagttaaaca agtagtttgt tcgttaggtg ttgctgccca tttaattaaa
     5941 tggggatatg atgcaaaaat aattacagaa ttgtattggg gagaaaaaac aaccattcaa
     6001 aatattcaat ttacggctgc gcctgcccgg catttttccg ggagaacaat aattcgcaac
     6061 cgcagcttgt ggagttcatt tattttgaga accgtatccc accaaattta tattggtggt
     6121 gattcgggtt atgatacaca ttttaaacaa ataggagagg actttggaaa atttgatctg
     6181 gcaattttgg agtgtggcca atataacccc gcttggaaat atattcacat gatgcctgaa
     6241 gaaacggttc aggctgccat cgatttaaat gccgacattt ttattccggt tcattggggt
     6301 aaattcgatt tatcgctgca tcattggacc gagcctatag agcgggctac agcacatgcg
     6361 caaaaactaa atatgcgtta ttttacacct atgatcggtg caatttcaca actgaataaa
     6421 gaaatgccaa cacataattg gtggagagat gttaagtaat aattttgaat tgaaatattt
     6481 taaggttggc aaaaaaatgc atgattatac tttcattttt attgttacaa gcaaacaaag
     6541 ccctgtttac ctatcaaagg tgcacgaatg tttattagtt tttagtcaag aaaaattgag
     6601 aggtatccga tcctgaaatt gccttaaaat aaattaatcc ctggtctaat gtatagtacc
     6661 gatataaagt tgatgatgtt gtagtatctg ttctattgtc aataaaatca aaggtagttt
     6721 gttcggtatt gcttataatt aaaccggata cccattggcc atctaaatct ttaatcgagt
     6781 ctatttcagc ttttccactg gcattaattg tcattaaata agaagtgtca gcaatacctt
     6841 taaaggaaat tgataatttc aaattgttat caaaaaatga gcatatttta gtagtgtcaa
     6901 agttgccttt aagttctact acttttccgg gtccaatggg ttcaaataac gattgtttta
     6961 ataattgaat attgttattt acaaaccatt tttcaactgc aaaattcatg ggtattgaat
     7021 cggaatttaa ccagtaggtt gaaattgtta atgtagtatc gtttattatt tggacatttt
     7081 gtttcaacag cacagttatt ggcagtgaat ctgaattgtg catatatgtt cgataaccgt
     7141 tggatggaat gcctgcagtt agataatcgg tcattgatat atcttttttt tgttctttgg
     7201 agcaagctga caataccata aataccccaa atagcgttat tattttcatc acctcaaaat
     7261 tacaaaaacc cgaaacattc atggaattcg tctaaattgg tgttgaggag tagcttcaac
     7321 aaaaaatgga tagtcaatgc tagtaagtgt ttcagcctgt gtgacttatg tcattgtcgc
     7381 tttcggcctt attttgtatc tttgataggt catcaatgtc gatggcgctc aattttaata
     7441 tgcagcagaa acagtccgtt aaatttatta atcgtgataa aagtttattt taccagacag
     7501 ttaaagagaa tgtaaataag tatttcaccg ataatcatat atccaaattt ggcaataata
     7561 cgatgtatat gaagtcgttt gtgttgctta cagcatacat cggagctttt tctctgattt
     7621 taatttttaa tccaccattt attgtcagta tgttgttatg gtttaccatg ggcattactg
     7681 ttgccggtgt tggaatgagt gtaatgcacg atgccaatca cggtgcttat gcagataaca
     7741 aatacgtgaa tatgatgatg ggttacacgc ttaatttatt aggtggaacc gtacataact
     7801 ggaaattgca acataatatc ttacatcata cttatactaa cattaccggt ttggatgatg
     7861 atattgatga taaggcaggt atgcgttttt caccacataa tacctggaag cctaaacata
     7921 attttcagta tgtatacgca tttttccttt acggtttaat tactttgtat tggtgtattg
     7981 ccaaagattt tgcgcagttt tcaaaatata ctgcaaatgg cgtaaatgca aattctaggt
     8041 cggcaaatat tatcatgttt ataaaactga tattcctgaa agctggttat ttctttttgt
     8101 ttttagtaat gccaactatg ttttttgaaa taccttttat tcaggtttta acaggcttcc
     8161 taattatgca ttttgtaggt ggaatgattt tgtcggtagt attccaatta gctcataccg
     8221 tagaaggaac ttcacatcca atgccttcaa ctgatggaac tatcgaaaat tgttgggcaa
     8281 ttcatcaatt gaacacaaca atgaatttct caagaaaaaa taaatggtta tcgtggtatg
     8341 ttggtggact aaattttcag gtggaacatc atttgttccc taaaatttgt cacgtgcatt
     8401 atcctgaaat tgcaccaatt gttaaagcta ccgccgaaca gttcaatatt ccttacctgg
     8461 aaagcaaaac ttttggctct gccttacaat ctcatattaa aacactaaaa cgtttgggca
     8521 aaatgcctgt tttagatgaa attatgggat gattttataa ctcaaatgtt ttaataaact
     8581 ttactgttac cgcctgattg tctactacag gtaaatgtgg gtctaatcgt gttacatcag
     8641 tgttggtgcc ttgattatag acgataaata agtcagtacc ttcctttgga ttatatcgga
     8701 aacgcagatt tgcactaact aaatcagata aatcatcgta ttgcacataa aaccgcagcg
     8761 atacttttgt agtgaagtta taggttacat tccatcgaat taaattactc acgaattgag
     8821 tgcttaccgt atcagttaaa taggtgtcga agttgatatt attaaattca tagtataggg
     8881 agaaattaag atgtgcattg atatagtaat taatatttgg cattaaagtc agtttttgac
     8941 cgctgtaaaa tccaccatat actccgatta atgcaagtcg aagcgcagat tgtgcaggcg
     9001 aataaatttc cagtgaatta ttccaggtgg tgtaagttcc ggctgcaatg gcattgttct
     9061 catccaatat ccagtcaaat ggcaatgagt ctattttata ttcaaacaac gacacattaa
     9121 tttccattcc attcttaaat tgaacacctg gccataggtc gattgcatgt gtttctcgtt
     9181 tgtgtgtatc aaatctccaa cgataattat tggtggagtt caaatacata tattgcaatt
     9241 tactcgattc gggaagtcca acccgatagg tgagttgcgt gcggctatta ccataattat
     9301 tatcatcaat aaatcccata tcagggcgaa tttcattacc gagaaaatcc aacgtgctgc
     9361 tgtaagtaaa tccggttaca gctgatttga aaacacccag atgtaaataa ctggcatcaa
     9421 tcaaatcatt agcctgtaaa ttaatgagag tgcttgctac accaccttca acagtaaatt
     9481 gctgggtaaa tctttttaca aagtccaagc ctacagactg attagaggtg ttagaagtat
     9541 cggtatttat tttattggta ataataccgc ctacaaaact ccctaaggaa tcgatatctt
     9601 ttctggtgcg caaaactgta aagttgtgtg gcgaaatacc ctcatctgca atacctagtg
     9661 tttgcatatt taaggcaccc atttgccagc cattcgcttt tcctgttaat cttgcacc
//
LOCUS       DAORKW010000073         9578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_168_length_9578_cov_5.912633, whole genome shotgun sequence.
ACCESSION   DAORKW010000073 DAORKW010000000
VERSION     DAORKW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168_length_9578_cov_5.912633"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2043
                     /locus_tag="PLB46_04655"
     CDS             <1..2043
                     /locus_tag="PLB46_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF75839.1"
                     /translation="YFVFEYKCSSAGVDLFFCDDIFIDPPFVDDVAPEIVSLEVLSST
                     QLTLTFNEPVTLESAETESFYVVDGGVGNPIDATRDVVDFSKVVLTFLSAFPEGVTLT
                     LTVNDVADENGNLIDNATQSFSIYTTQEYDLLIDEIFADIEPQVGLPLGEYVELYNNT
                     DVEIDLVGFQLHDATSESDAFTNYIIPAGGYVVLVDDGVADLFSAYANVLVVTGLPSL
                     NNDGDDLQLISPEGTVIHAVNYKIDWYGNAVKEDGGWSLEMIDTNNPCQEKENWKAAI
                     DANGGTPGLVNSVAADNPDETAPALIEAYPIGLDTVITTFDEKIILDGLSTSNFTITT
                     STGETIIPTALIIDPNNAFIVGLKMPTTLLFGIIYTVSATGVSDCSGNEINLFNQAIF
                     GLPEPALANDVVINEVLFNPISEGVDFVEIYNRSAKIIDLSTLIIAEMDLFDTTEVNE
                     YDNISLSGRLLLPGQYLCLSSDPDLIAAQYFSIDTGNFLMVNELPNYADNEGIVAILD
                     NTLNEIDRLHFYDDWHYALLSNQDGVSLERINYDYTTQDNNNWFSAASDVHFATPGYQ
                     NSNFGAYTSSGTVAVSSPVFSPDGDGFNDLMVITYSTETEGFTGRFQIFSAQGKLVKD
                     LTGNITLSREGFITWDGLNNDDQKASSGIYILYAELFDLEGNVKSYKLKSTLVRKQ"
     gene            complement(2047..3237)
                     /locus_tag="PLB46_04660"
     CDS             complement(2047..3237)
                     /locus_tag="PLB46_04660"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF75840.1"
                     /translation="MNIPNSLRQIYLLTVLICFSVAGFAQNNHIKIGDTLRLESYQLT
                     ALNNTGLQKDKPLVIDFWATWCGPCIAAFPHLDEITTKYKNNVQVIVLSDEKSERVNN
                     FIKNNNYNFDYFIDNNKSVFTLFQIDARPLTCFIDPTGKLLWVGDSKNLEQVIEDYLL
                     NNSVGSNIATGALAGKFYTTNEVPVKSNNLMLYQIGYSIDPDNYFVRSQKGHRVDSAI
                     NIEYRGVTVSELLQDMLNLPVLQFTNTVEKLDTIYIDLIAISENPAMTYKVVTGKIKT
                     DLGLLFNFSIISNKREVALYELTIADEKKLKQYFEPVEGGGMVEIIGDSTTITRLSLE
                     QIAYHLEKKYKTFFSYNGDDTGKYSFTYKNVTSITEMEKQLFEIIGIKFETEMAVKEF
                     ITIQ"
     gene            complement(3471..9542)
                     /locus_tag="PLB46_04665"
     CDS             complement(3471..9542)
                     /locus_tag="PLB46_04665"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRF75841.1"
                     /translation="MQNPDNGNWNGYEKEWAIVDSLEGQGLPASALKAAEAIYVYAKN
                     EKNDEQIVKAIIHKAKYTSMVEENEQIKAIHLFQEEIETSSGVIKSVLQSMTAEIYWN
                     YYTNNRWRFNNRSKTVDFNNEDISTWDVTTLHQKTYDLYVASIANREELKKVKTSRYN
                     EILIKGDEYGQMLRPTMYDFLAHRALDFLMNDEVYITDPAYAFNIDNPVVFGSNKAFA
                     GFVFSAEEVESKKLLALRIFQDLTLAHLYDPYPAALIDVDIKRLNFAKNNTIINNKDS
                     LYLNALIAEEKLYANDSSSTRISFLIAQHHYSLGSKYVPLAGPEYKWEYETAIKICDD
                     AIKKFPNSLGAINCASLKATIEQKELTLVAEQINVPEKPFRVGVGFRNVDTVFLRVIK
                     VSQKDIDKIKVMEYDVKFNELTKYPVYKSWSQQLPLDGDYQDHFTEIKVDGLPLGSYY
                     LIASTNAAFTFSENKLIAQEIIISNLSYFASGNDYYGTPNKDFYVLDRSTGKPIKGAS
                     IQTFRKEYDYNKRDYNYNPLKTYTTDENGMFSVSDPLIKDYYYFSFSITYNKDFLSSG
                     DNYYISKENKPNTNSTFQTYFFTDRAIYRPGQTIYFKGIMVERSYDGKKQTVVANKKS
                     TILFYDVNSQVVAKIDVTTNDYGSFNGTFTTPQTGITGQMRISNETGSTFFSVEEYKR
                     PTFSVSNDPVTGGFVLGDTITVTGKAISYAGANIDNAKVAYRVVRNTYYPYYYSDYYW
                     WRKPAPVSAEMEITNGTSTTDAEGKFAVTFKAIADLTADKKLLPHFSYTVYVDITDVS
                     GETRSQETYVTVGYTALNLFVNAPETINTGKNVTLNITTNNLNGSRITANGKVEIFAL
                     ETPNKLFRERTWQQQDVYTMSKEEYYKLFPHDLYADESNMYAWPVTGSVLKAEWSTEK
                     SDSLVLNTAALKQGVYKIEVSAIDISGEVIKVIKYISVLNQGNYAATPKYNFTTTESK
                     SYKPGENAVINIGSSAKNINALLEITRADGKAARKWITLPEVGNRGSNATVMDIEIPI
                     SESDRGGVSAQICFIKDGRFYNQQYFISVPWDNKDLKLTLETHRDKLQPGATETWKIK
                     ITRNNGEKVAAELLANMYDASLDAFVGHYWNSVSWPSSYTYKYWTGVGFGNGENLVYT
                     DYDWYKRVSGLTFNYTTLNWFGIYWGVSYYRDYYFADGDMDRLDNRSFAGAPVLMETA
                     VEKSKMLESEESMGLGAIANGKVSIEPVTNSNTLSNNSLEQVGTRTNLNETAFFYPHL
                     ETDAEGNIAFTFTMPEALTTWNFMGFAHTKEMQSAQLFDKVVTQKELMIQPNAPRFLR
                     EGDEIVFTAKVSNLTENELNGNASLELFDALTMQPIDNIFGNTANMVAFKALAKGSAA
                     VSWKLKIPSGYDAIVYKVKAASGTFTDGEENALPVLSNRILVTEALPLWVRGNQSKLF
                     TFDKFLAAKGSSTIRNQSLTLEYTSNPAWYAVQALPYMMEYPYECAEQVFSRYYANSI
                     AYHIANSDPEIKRVFDSWKNNATSLTSNLEKNQELKQLLLAETPWVLQAQNETERKKR
                     VALLFDLNRMNNELQSAINKLEKMQLPNGGWPWFKGYEDDRYITQYIVTGLAHLQQLG
                     ITNANTDAQVSRMLNNAMAYCNNRMVEDYRNLEKYKVDKATHVPGNITVQYLYAASYF
                     GDDVVALSSEQKIAKDYYIGQLVKYWTKYSIYEKGMIALILHREDQSKTAASIVKSLK
                     EFSLNSDEMGMYWKDNLAGYYWYQAPIETQSLMIEVFGEVGKDEKAVEDLKVWLLRQK
                     QTTDWKTTRATAEACYALLLRGNNLLSNKELAIITVGKLLVKPESVEAGTGYFKTNWL
                     GTDINNDYGKISITPPSTNSLSYGAMYWQYFEDIDKVTASNNNLQINKQLFLQQNTAT
                     GPVIKPITDKTELHVGDLIKVRIEIRVDRNMEYVHMKDMRASGFEPVNVLSQYKWQDG
                     LGYYEATGDAATNFFIAYLHKGTYVFEYPLRVAHSGNFSNGITTIQCMYAPEFTSHSE
                     GIRVQVK"
BASE COUNT         2843 a         1733 c         1663 g         3339 t
ORIGIN      
        1 tattttgttt ttgaatataa atgctcgtct gctggtgttg atttattttt ttgcgatgat
       61 atttttatcg acccgccatt cgtagacgat gtggctcctg aaattgtttc attagaagtg
      121 ctttcttcta cgcaactcac tttaactttt aatgaacccg taacgcttga gtcagccgag
      181 acagaaagtt tttatgtggt tgatggcggt gttggcaatc cgatagatgc aacgcgcgat
      241 gttgttgatt tttcgaaagt tgtgttaact tttttaagtg catttcctga aggtgttacc
      301 ttgactttaa ctgttaatga tgttgcagat gaaaatggca atttaataga taatgccaca
      361 caatcatttt ctatttatac tacacaggaa tatgatttat taattgatga aatttttgca
      421 gatatagaac cacaggttgg cttaccccta ggtgaatatg ttgaattata taataatact
      481 gatgttgaaa ttgatttagt tggttttcaa ttacacgatg caacgtcaga aagtgacgca
      541 tttactaatt atattattcc tgcaggtggc tatgtggtgt tggttgatga tggcgttgcc
      601 gatttgtttt ctgcctatgc taatgtttta gttgtaaccg gtttgccttc attaaataac
      661 gacggcgatg atttacaatt aattagtccg gaaggcactg ttatacatgc tgtgaattat
      721 aaaattgact ggtatggcaa tgcagtaaaa gaagacggcg gctggagtct cgaaatgatt
      781 gacacaaaca acccgtgtca ggaaaaagaa aactggaagg cggcaattga tgccaacggt
      841 ggaactccgg gattggtaaa ttcagttgca gcagataatc cggatgaaac agcaccggct
      901 ttaattgaag cttatcctat aggattagat acggtaataa ctaccttcga tgaaaaaata
      961 atattggatg gattaagcac atcgaatttt accataacta cttcaacagg tgaaacaata
     1021 attcctacag ctttgataat tgaccccaat aatgcattta ttgttggctt aaaaatgcca
     1081 actacattat tgtttggaat tatttatacg gtaagtgcaa caggagttag cgattgcagt
     1141 ggcaatgaaa ttaatttatt taatcaggct atttttggac taccggaacc tgcattagca
     1201 aatgatgttg taattaatga agtattgttt aatccaattt cggaaggtgt agactttgtt
     1261 gaaatttata accgttcagc aaaaattatt gacttatcca ctttaattat tgctgaaatg
     1321 gatttgtttg ataccactga agttaacgaa tatgataata tttcactaag tggcagattg
     1381 ttattgccgg gacaatattt gtgtctttcc agtgaccctg atttaattgc cgcacaatat
     1441 ttttcaattg ataccggaaa ttttcttatg gtaaatgagt tgccaaatta tgcagacaac
     1501 gaagggatag tagctatttt agataataca ttaaatgaaa ttgacagatt acatttttat
     1561 gatgattggc attatgcatt attaagtaat caggatggtg tttcgttaga acgcattaat
     1621 tacgattata ctacgcagga taataacaat tggtttagtg cagcatctga tgtgcatttt
     1681 gctactccgg gatatcaaaa ttctaatttt ggggcctata cttcgtctgg tactgttgct
     1741 gtttcatccc cggtgttttc gccggacgga gatggattta acgatttaat ggtaataacc
     1801 tatagcacag aaacagaagg atttaccggt cgtttccaaa ttttttcagc tcaggggaaa
     1861 ttggttaaag atttaacagg aaatattaca ctaagccgtg aagggtttat tacctgggac
     1921 ggactgaata acgacgacca aaaagcatca tcaggcattt atatattata tgctgagtta
     1981 ttcgatttag agggtaatgt taaatcgtat aaattaaaat ctacgttggt acggaagcaa
     2041 taatttttat tgtatggtaa taaattcttt aacagccatt tctgtttcga attttatgcc
     2101 gataatttca aataactgtt tttccatttc tgttatactc gttacgtttt tataagtaaa
     2161 cgaatatttg ccggtgtcgt caccgttata actgaaaaat gttttatatt ttttttcgag
     2221 atgataagca atttgttcaa gcgacaatct tgtaatagtg gtgctgtcac caataatttc
     2281 caccatacct ccaccctcaa ccggttcgaa atattgtttt aatttttttt catctgcaat
     2341 ggttaattca taaagggcaa cttccctttt gtttgatata attgaaaaat tgaataataa
     2401 ccccaaatcc gtttttattt ttcctgtaac cactttatag gtcatagccg ggttttcgct
     2461 tattgctata aggtcgatat aaattgtatc taatttctct acggtattag taaattgtaa
     2521 aacaggtaaa ttaagcatat cctgaagtaa ttcactcacc gtaacgccgc gatattcaat
     2581 gttaatggcg ctatcaacac gatgaccttt ttgtgaacgg acaaaataat tatccgggtc
     2641 aatgctataa ccaatctggt ataacattaa attatttgat ttaacgggca cttcatttgt
     2701 ggtataaaat tttccggcca gtgcgcctgt agctatgttt gaaccaactg aattattgag
     2761 caaataatct tcaattacct gttcaagatt cttactatca ccaacccata agagtttgcc
     2821 ggtagggtca ataaaacagg taagtggccg cgcatcaatt tgaaatagtg tgaaaacaga
     2881 tttattgtta tctataaaat aatcaaaatt gtagttattg tttttgataa aattattaac
     2941 cctttcagat ttttcatcac ttaatacaat tacctgaaca ttatttttat attttgtcgt
     3001 tatttcatct aaatgtggaa acgctgcgat acatggccca caccaggtag cccaaaaatc
     3061 aataaccagc ggtttgtcct tttgtaatcc ggtattattc agagcagtaa gttgatagct
     3121 ttcaagacgt aacgtatcgc caattttgat gtggttgttt tgagcaaaac ctgcaacgct
     3181 aaaacatatc agaactgtaa ggagataaat ttgacgcaaa ctatttggaa tattcatact
     3241 gcaaaaatat taatttgaaa aaaaattgtt aaatttttat tcaaatctta gattgccatg
     3301 acaatgcaaa taacgcttta aaaataagta ggaaaagtaa aatttggaac gaattaccga
     3361 gctcaggaac cggccttaca aatacacaaa aaatcagcta ttagcatttt ttgcttcaat
     3421 cataaataaa aaagcgcact aagcttatta cttggcgcgc tttttataat ttatttcacc
     3481 tgcacccgaa taccttcgct atggctggtg aattccggag catacataca ttgaatagta
     3541 gtaataccat tgctgaaatt cccgctatgt gctacacgca gaggatattc aaatacatag
     3601 gttcctttat gtaaataagc gataaagaaa ttagttgcag cgtcgccggt ggcttcataa
     3661 tatccgagac catcttgcca tttgtattgg ctcaatacat taaccggttc aaaaccgctg
     3721 gcgcgcatat ctttcatgtg cacatattcc atatttctgt ccacacgaat ttcaattctc
     3781 actttaatta aatcgccaac atgtaattcg gttttgtcgg ttatcggttt tattaccgga
     3841 ccagttgcgg tgttttgttg taagaacaat tgcttattaa tttgtaaatt attatttgaa
     3901 gcagttactt tatctatatc ttcaaaatat tgccaataca tagcaccata actaagcgaa
     3961 ttagttgaag gcggggtaat actaattttt ccgtaatcgt tattaatatc tgtacccagc
     4021 cagtttgttt taaaatatcc ggttccagct tctacacttt caggtttaac caataattta
     4081 ccaacagtaa ttatcgccag ttctttattg cttaataaat tatttccgcg aagcaacaag
     4141 gcataacatg cttcagcagt tgcgcgtgtt gttttccaat cggtagtttg tttctgacga
     4201 agcaaccaca cttttaaatc ctctacggct ttttcatctt tacccacttc tccaaacact
     4261 tcaatcataa gcgattgggt ttcaattggt gcctgatacc aataataacc tgctaggttg
     4321 tctttccaat acatacccat ttcatcgctg tttaagctaa attcttttaa tgatttaact
     4381 attgatgcgg cagttttact ttggtcttca cgatgtaata tcaaggcaat catacctttt
     4441 tcataaatac tgtattttgt ccagtattta actaattgcc cgatgtaata gtctttggca
     4501 attttttgtt cgctgctcaa ggccactaca tcatctccaa aataactcgc agcatataaa
     4561 tattgaacag taatgtttcc gggaacatgt gttgctttgt caactttata tttttctaaa
     4621 ttgcgataat cttcaaccat tctgttatta caataagcca tggcattatt taacatgcgg
     4681 ctaacctgtg catcggtatt agcattggta atacctaatt gttgtaaatg cgcaagccct
     4741 gttacaatat attgcgtgat atatctatcg tcttcatatc ctttaaacca tggccagccg
     4801 ccatttggca attgcatttt ttccaattta ttaatggcag attgcaattc gttgttcatt
     4861 ctgtttaaat cgaataataa tgcaacacgt tttttgcgtt cggtttcatt ctgtgcttgt
     4921 aatacccatg gtgtttctgc taataataat tgttttaact cctgattttt ttcgagatta
     4981 gaagttaatg atgttgcatt atttttccat gaatcaaaca cacgtttaat ttcagggtct
     5041 gaattcgcga tatgataagc aatactattt gcataataac gactaaacac ttgttcggcg
     5101 cattcgtaag ggtattccat catataaggc aaagcctgca cagcatacca tgccgggttg
     5161 ctggtgtatt ctaacgtaag actttgattg cgaatagttg aagaaccttt tgcggccaaa
     5221 aatttgtcga aagtaaataa tttagattga ttaccgcgca cccataaagg caatgcttcg
     5281 gttactaaaa tgcgattact taaaaccggt aaggcgtttt cttcaccatc ggtaaatgtt
     5341 cctgatgctg cttttacttt atacacaata gcatcataac cggaaggaat ttttaatttc
     5401 caacttactg cagcacttcc ttttgccagt gctttaaacg caaccatgtt ggcggtattg
     5461 ccaaaaatat tatcaatagg ttgcatggtt aatgcatcaa ataattctaa agatgcattt
     5521 ccgtttaatt cgttttcagt taaattggaa actttagcag taaatacaat ttcatcgccc
     5581 tctcttaaaa atctcggagc attaggttgt atcattaatt ctttttgtgt aacaacctta
     5641 tcgaataatt gcgcactttg catttcttta gtatgcgcaa atcccataaa attccaggta
     5701 gttaacgctt caggcattgt aaaggtgaaa gcaatattac cttcagcatc tgtttccaaa
     5761 tgaggataaa aaaaggctgt ttcatttaaa ttggttctag tacctacctg ttccaggcta
     5821 ttattggata atgtgttcga atttgtcaca ggttcaattg aaactttacc attggctatt
     5881 gcacccaacc ccattgactc ttcactttcc agcatttttg atttttcgac agcggtttcc
     5941 attaaaacag gagctccggc aaaacttctg ttatccaaac gatccatgtc accatcagca
     6001 aaataataat cacggtaata actcactccc caataaatac caaaccagtt tagggttgta
     6061 taattaaatg ttaggccgga tactcttttg taccaatcgt aatcggtata cactaaattt
     6121 tctccattgc caaaaccaac acctgtccaa tatttatagg tataagacga tggccatgaa
     6181 acactattcc aataatgtcc gacaaaagca tcgagagaag catcatacat atttgccaat
     6241 aattctgctg ccaccttttc accgttgttg cgtgtgattt ttattttcca ggtttcggtt
     6301 gctccgggtt gtaatttatc gcgatgtgtt tcgagggtta attttaaatc tttattatcc
     6361 caaggcacac tgataaaata ttgttgatta taaaaacgac catcttttat aaaacaaatt
     6421 tgtgcggata caccacctct atcgctttcg cttattggga tttcaatatc cattactgtt
     6481 gcattacttc cacggttacc aacttcaggc agggtaatcc attttctggc agcttttcca
     6541 tcggctcggg taatttctaa taaggcatta atatttttag cgctactgcc aatatttatt
     6601 acagcatttt caccgggttt atacgatttg gactcggtag ttgtaaaatt atattttggt
     6661 gtagctgcat agttgccctg atttaataca ctgatgtatt ttatcacttt aataacctca
     6721 ccactgatat caattgcact tacttcaatt ttatatacac cttgctttaa tgcagcagta
     6781 tttaatacca gcgaatctga tttttctgtc gaccactctg ctttaagcac agaacctgtt
     6841 accggccatg catacatatt ggattcatca gcatataaat cgtgtggaaa taatttataa
     6901 tattcctctt tgctcattgt gtatacatcc tgttgctgcc aggttctttc tctgaataat
     6961 ttattaggcg tttccagtgc aaatatttcc acttttccgt ttgcggtaat tcgtgaaccg
     7021 tttaaattat tggtggtaat atttaaggtt acatttttac cggtatttat ggtttcaggt
     7081 gcatttacaa ataaattgag tgcggtgtaa ccaacagtta cataagtttc ctgacttctg
     7141 gtttcacctg acacatctgt tatatcaaca taaacggtat aagaaaagtg aggcaataat
     7201 tttttgtcgg cagttaaatc ggcaattgct ttaaatgtta ctgcaaattt tccttctgca
     7261 tcggtggttg aagttccgtt ggttatttcc atttctgcag aaacaggagc cggtttgcgc
     7321 caccaataat aatcgctgta atagtaggga taataggtgt ttcttaccac gcggtaagcc
     7381 accttcgcat tatcaatatt tgcacctgcg taagatattg cttttcctgt tacggtaatt
     7441 gtatcgccaa gcacaaaacc tccagttacg gggtcgttac taaccgaaaa tgtagggcgt
     7501 ttatattctt ccacagaaaa aaatgtgctt ccagtttcgt tgcttatgcg catttgtccg
     7561 gtaatacctg tttgcggcgt tgtaaacgtt ccgttaaatg agccataatc atttgtagtg
     7621 acatctattt tggcgacaac ctggctattc acatcgtaaa atagaatagt tgatttttta
     7681 ttcgcaacaa cagtttgttt tttaccatcg taactgcgtt caaccattat ccctttaaaa
     7741 taaatggttt gtcccgggcg atatattgct ctatcggtga aaaaataggt ttgaaaggtt
     7801 gagttagtat taggtttgtt ctctttgctg atataataat tatcaccgga acttaaaaag
     7861 tctttattat aagtaatact aaagctgaaa taataataat cttttatcaa tgggtcgcta
     7921 acagaaaaca ttccattttc atcagtagta tatgttttta aaggattgta gttataatct
     7981 cttttgttat aatcatattc ttttctgaat gtttgaatac ttgcaccttt aatgggttta
     8041 ccggttgatc tgtccaacac ataaaagtct ttattaggag taccataata atcgttaccg
     8101 gaagcgaaat acgataaatt tgaaataatg atttcctgag caattaattt attttcgcta
     8161 aatgtaaacg cggcattcgt gctggcgatt aaatagtaac ttccaagcgg taaaccgtct
     8221 acttttattt cagtaaaatg gtcctgataa tcgccatcca aaggcaattg ctgactccat
     8281 gatttataaa caggatattt agtaagttcg ttaaacttaa catcatattc catcacttta
     8341 attttgtcaa tgtctttttg acttacttta atcacacgta aaaaaacagt atcaacattg
     8401 cggaaaccta caccaacccg aaaaggtttt tccggtacgt ttatttgttc ggcaaccagt
     8461 gttaattctt tttgttcaat tgtagctttt aacgaggcac aattaatggc gccaaggcta
     8521 tttgggaatt ttttaatggc atcatcacaa attttaatag cagtttcgta ttcccattta
     8581 tattccgggc cggctaaagg aacatatttg ctaccaaggc tgtaatggtg ttgagcaatt
     8641 agaaaactaa ttcttgttga actactgtcg tttgcgtata atttttcttc tgcaattaat
     8701 gcatttaaat acaaactgtc tttgttatta attatcgtat tgttttttgc aaagtttaat
     8761 cgtttaatat ccacatcaat taatgcagcc ggataagggt cgtacaaatg tgctaaggta
     8821 agatcctgaa aaatgcgcaa tgcaagtagt tttttagact ccacttcttc ggcagaaaac
     8881 acaaatccgg caaaagcctt attactgcca aacactacgg ggttatcaat attaaatgca
     8941 taagccgggt cggtgatata cacttcgtcg ttcattaaaa aatctaaagc gcgatgtgct
     9001 aaaaaatcgt acatggtagg gcgtaacatt tggccatatt catcaccttt tattaaaatt
     9061 tcgttgtaac gtgaggtttt aacttttttt agttcttctc tgttagcaat agaggccaca
     9121 tataaatcgt atgttttttg atgtaatgtg gtaacatccc aggtgctgat atcctcatta
     9181 ttaaagtcaa cagttttact tctattgtta aaacgccatc tgttattggt ataataattc
     9241 caataaattt cggcagtcat actttgtaat acacttttaa ttacacctga tgaagtttca
     9301 atttcttcct ggaataaatg aattgctttt atttgttcat tttcttccac catactggtg
     9361 tatttggctt tatgtataat tgctttgaca atctgctcgt catttttttc gtttttggca
     9421 tacacataaa ttgcttcagc agctttgagg gcagaagccg gcaatccctg accttcaaga
     9481 gagtctacaa ttgcccattc tttttcgtaa ccgttccagt taccgttatc gggattttgc
     9541 atagtttgat aggttatagc tgaaaccaaa gctccggc
//
LOCUS       DAORKW010000074         9541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_170_length_9541_cov_5.739933, whole genome shotgun sequence.
ACCESSION   DAORKW010000074 DAORKW010000000
VERSION     DAORKW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170_length_9541_cov_5.739933"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLB46_04670"
     CDS             complement(<1..441)
                     /locus_tag="PLB46_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75842.1"
                     /translation="MNRILIVCMMIFLAWPSFVQAQQIFISDPQKISTELIGYNILGK
                     NKNGDVLIYKKYRFEDEIDIYDKQMSFKRRKEITIRSLDYETVEVYKTGDNIYHFYTH
                     KDNKMQYLSVQHYNSDLEKKGVPVLLDSTSQKLGDSFTEFKITKA"
     gene            complement(580..1908)
                     /locus_tag="PLB46_04675"
     CDS             complement(580..1908)
                     /locus_tag="PLB46_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF75843.1"
                     /translation="MSKKNNDNQFSFRKYAMKQFKHNKPAYVSVYILLFFSVIAIFAP
                     ILANEKPLYIHYKGEHFFPAFSFKNNYYINNVDGSSEKIQLDIADWKQMQFDKVVWAP
                     IPYSPGKSDRLNSGYKSPGGEQFFKDKSGAVVPIPGRFRHILGTGSRGDDLMAGLIHG
                     ARISLTIGFISMAIATLIGLFLGSMAGYFGDNKLTTSRGRFWMVVIGIFVAWFYGFQV
                     RQFVLQEAIKTSGITLLLQLLLSIFIVVAVIFLFSQLGRLVGKLPWLNSKVNIPVDGL
                     VSRTIEIFHSMPTFILILTIAAIARPSLTNIMIIIGLTSWTGIARLTRAEFLRIRNLE
                     YIQAARSLGYNEYTIMFKHALPNGMAPALVSIAFGIASAILVESSLSFLGIGVPSDVV
                     TWGSLVNDGREKFSAWWLVIYPGLAIFITVTVYNLIGEGLRDALDPKQKR"
     gene            complement(1912..3411)
                     /locus_tag="PLB46_04680"
     CDS             complement(1912..3411)
                     /locus_tag="PLB46_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF75844.1"
                     /translation="MGKYLLKRILFFIPTLIIISLLAFIISINAPGDPLDRMVSAQES
                     GGEFSTQTANTAKEKERWRKKLGLDLPVFYVSLSNAATPDTLYKVYDDNERTALKRLI
                     SQYGNWENIAAWNNQLHALSTAINRMIPDTSNFAVYGKNFVLDTIIEAKQNIGVLMYS
                     YEEAPIQLRLNTLAHYAATYPYFEPIKAQILKTKALYEQLISEKQIAKTWIPAIHFYG
                     YNQYHRWIFGDGNWLTGKGSVDCKGLIRGDFGYSYETKLPVGNVIGERIKWSLFFALT
                     SVILAYLISLPIGIKAAAKKNSPFDKTSSVILFVLYAMPTFWVATILLMSFANVEALH
                     IFPASGIQPVTGIPDDASWLTTIKMRLPYLILPTIAFTYSQLAFLSRITRVSTLEIIA
                     QDYIRTARAKGLSEQTVIYKHAFRNALLPIITVFSNIFPMAISGSVILETIFTIPGMG
                     EQVFHAITTKDYPVIIDVFTLTGILTLLGYLVADILYAVADPRISYASK"
     gene            complement(3475..5367)
                     /locus_tag="PLB46_04685"
     CDS             complement(3475..5367)
                     /locus_tag="PLB46_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF75845.1"
                     /translation="MSTKFPVLSTLTIATAFLLASCGGNGGNNKNDISTLNVSDKIRN
                     KRIADATNILPEWSKENIVVNHWPGDPDNLHPTNGGTASRSWILQLTANYILRNDVIN
                     LNVCPDLAEAMPEVSDDNLRYTYTLRKNATWDDGSAITAEDCVFMLKANRCPLTNNPI
                     TKSYFENIQDVITYPDNAYKFTIVMKQEEINNINFLSDYPVFQRKYYDPNNVLAKYTF
                     AQFNNPDFNTDNEKDLLAWANKFNDAALGNDISLLNYSGAYKVAEWTRGQNLILERKK
                     NHWTQKLSNPNEYETSFPEKIIFKIIKDPTAQKLEFGSQTLDASVWLSTVTATELMDG
                     GNFLTHYNIEFTDNYSYNYIGLNMKPDGVTHKKFFDDVKVRRAMAYLVPVDDLINVIT
                     LGRAKRMAGPISPLKDEYNTNLKPLPNDIAAATKLLDEAGWKDSDGDNIRDKVIDGVK
                     TNMQIEFKYQAGQTFVEETAIMIKEAVYKGGIELILIPIEGNTLKEHLQKHDFDMYMS
                     AWAGGSLPEEFTQLWHTKSYSTGGSNYVGFGNSETDALIDQIKVTMDAEKRIPLSQKM
                     QQIIYDEQPYIFLYSAYRKNIIHKRFGNQVMTFDRPGNILNHLRLIALYGAQSGTATN
                     ATESNQ"
     gene            5472..6374
                     /locus_tag="PLB46_04690"
     CDS             5472..6374
                     /locus_tag="PLB46_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRF75846.1"
                     /translation="MKKVLISGGSGLIGRHLTGILLQNGYEVAWLSRSVKPLKDVKMY
                     KWDVDAGYIDPEAILWADVLVHLAGESIAGKRWTAKRKQEIIESRTKSTELLITAMQQ
                     HNHHIGQVVAASAIGFYGETGDLPVDETNASGNGFLSESVRQWENATTKFSALGINLV
                     TLRIGIVLAENGGALKEIVQTKSLRVLPIMGSGKQIYAWIHIQDVGQIMLYSIQQKLT
                     GVYNAVAPHPVSLKSIIQHYKQVSGKWYLQMPVPAFALKLVLGELSAVVLTGQNVSCA
                     KIQSAGYQFSFPDITSALTQIITT"
     gene            complement(6427..8385)
                     /locus_tag="PLB46_04695"
     CDS             complement(6427..8385)
                     /locus_tag="PLB46_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HRF75847.1"
                     /translation="MQKLLFPSLLVVLIASGILIGAESNRTPEVDFTGTSKIFGLIVE
                     STYGTPLVSAELTLDNKTKFHPDENGLYSIEKLAAGKYTLQVSATGYVAQKIPIEIAD
                     GESKEINISMAMQVSVSGDSLQVLQIKENVEDKKGEVACKVVAQSNSVSFDAISYMSL
                     SIDEEFHGGEVVHNTEAYDVINENAFKTVVGNPLSTFSIDVDNAAYANIRRFITYGQT
                     PPKDAVRIEEMLNYFDYTYAAPKGNDPFAINYEMSECPWNTESKLVMIGLQGKDIDYS
                     EAAASNLVFLIDVSGSMSDQNKLPLVISSLNTLVDNLNEKDRVALVVYAGAAGEVLPS
                     TPCNQKSTIKNALNNLQAGGSTAGGAGIKLAYEIAVKNLIPGGNNRVILCTDGDFNVG
                     ASSDAEMTRLIEEKRKTGVFISICGFGMGNYKDSKMEKIADNGNGAYYYIDSEKEAKK
                     VFTTDMRGTLFTIAKDVKIQIEFNPALVESYRLIGYENRILAKEDFDDDTKDAGELGA
                     GHKVTALYELKLRKSAYQPSGINSTSGTVEDLKYQTTSIKNDAYNTDEIMTLKLRYKA
                     PNSETSKLIEKSLFKTTVPFASTSDNFRFASAVVEFGMLLRDSEFKGDASYDEVLKLA
                     SSATGADANGYRAEFISLVKTFNGLAKK"
     gene            complement(8605..8955)
                     /locus_tag="PLB46_04700"
     CDS             complement(8605..8955)
                     /locus_tag="PLB46_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75848.1"
                     /translation="MKTLTVFLLAISMMLSSCYNVDELPETNKITYIVTGTQFDVIYK
                     DETGNNQYVYDREGVFKVYIEDIVGDPVFILAKGSDDLKVHIHVKDGIDTVRKAKDAG
                     SELVYIDLGSSRVE"
     gene            complement(9071..>9541)
                     /locus_tag="PLB46_04705"
     CDS             complement(9071..>9541)
                     /locus_tag="PLB46_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRF75849.1"
                     /translation="AVRDAVTIPVIAAGGIGDGRAMLACMALGADGVQVGSRFAASIE
                     SSAHINFKQEIIQTDEGSTTLTLKEITPVRLIKNKFYQEVEAAYQRCAGPDELKTLLG
                     RGRAKKGMFEGDLAEGELEIGQVSATIRDILPAATILQEIIKGFEAAKLRLLAD"
BASE COUNT         2924 a         1855 c         1691 g         3071 t
ORIGIN      
        1 tgctttggtt attttaaatt cggtaaaact atcgccaagt ttttgtgatg tagagtccaa
       61 taacaccgga acaccctttt tttcaaggtc gctgttgtaa tgctgcaccg acaggtattg
      121 cattttattg tccttgtgcg tgtaaaagtg ataaatatta tctccggttt tatatacttc
      181 tacagtttcg taatctaaac ttctgatggt aatttctttg cggcgcttaa aactcatttg
      241 tttatcataa atatctattt catcttcaaa acgatatttc ttatagatta aaacatcacc
      301 atttttattt ttaccaagaa tattataacc gattaattcc gtgcttattt tttgggggtc
      361 agaaataaat atctgctgag cctggacaaa agagggccaa gccaaaaaaa tcatcataca
      421 tactatcaat attcggttca cgttcacgaa tttatctaat aactgcttct gccggataat
      481 tattttcaaa attacataaa aatagcttag ttccttcaca ggattataaa atggcaaagt
      541 tgtgatgatt gcctaaaatg gaattgtaca gaataaaaat taccgttttt gtttagggtc
      601 aagtgcgtct ctaagccctt ctccaataag attgtaaacg gttactgtga taaatatggc
      661 taaacccgga taaataacta accaccaggc actgaacttt tcacgaccat cgtttaccag
      721 cgaaccccat gttaccacat cagacggcac accaatgcct aaaaaggata aactggattc
      781 aaccagaatt gcagatgcaa taccaaaagc tatacttacc agagccggtg ccattccatt
      841 tggcaaggca tgtttaaaca tgatagtata ttcattatat cccaaagacc tggcagcctg
      901 aatatattcg agattgcgta tgcgtaaaaa ttcagctcga gtaagtctgg caattcctgt
      961 ccacgatgtt aatccaatta taatcataat attggtcaac gaaggtcttg caatagcagc
     1021 tatagtgagt attaaaataa aggttggcat gctatggaat atttctatgg tgcgggacac
     1081 taatccgtca accggaatat tcactttaga atttaaccat ggtaatttgc caactaaacg
     1141 gccaagttgt gaaaacaaaa atataacggc tactacaata aaaatactga gtaacaattg
     1201 taatagtaaa gtgatacccg atgttttgat ggcttcctgt aacacaaatt gcctaacctg
     1261 gaaaccataa aaccacgcaa caaaaatgcc tattactacc atccagaaac gtcctctgct
     1321 ggttgttaat ttattgtcgc caaaataccc cgccatcgac cctaaaaata atcctatgag
     1381 tgtggcaatt gccattgaaa taaatccgat ggttaatgaa attcgtgcac cgtgaattaa
     1441 tccggccatt aaatcgtcac cgcgactgcc tgtacctaaa atatgtcgaa acctgcctgg
     1501 gatgggcact acagcaccac ttttatcttt gaaaaattgt tctcctcccg gtgatttata
     1561 gcctgaattt aacctgtcac ttttgcccgg tgaataaggt atgggcgccc aaacaacttt
     1621 atcgaactgc atttgtttcc agtcggcaat gtctaactga attttttcag aactgccatc
     1681 aacgttgttg atgtaataat tatttttaaa ggaaaatgca gggaaaaaat gttctccttt
     1741 ataatgaata tataaaggtt tttcgttagc taaaatagga gcaaaaatgg caattactga
     1801 aaaaaacaat aatatataca ccgaaacata agcaggttta ttatgtttga attgcttcat
     1861 cgcatatttc ctgaaactga attgattatc gttgtttttt ttgctcatgc tttattttga
     1921 tgcatacgaa attcgagggt ctgctactgc atataaaata tcagccacta aatatcccaa
     1981 taaagttaat ataccggtta aggtaaaaac atcaataatt accggataat ctttggtagt
     2041 tatggcatga aatacctgct cgcccatacc cggaatggtg aaaatagttt ccagtattac
     2101 agaaccacta atcgccattg gaaaaatatt ggaaaaaacc gtgataatag gcaataacgc
     2161 atttctgaac gcatgtttat aaataacagt ttgctctgaa agtccttttg ctcttgctgt
     2221 gcgaatataa tcctgcgcta taatttccaa agtggaaaca cgtgtaatac gacttaaaaa
     2281 tgctaattga gagtaggtaa atgcaatagt aggcaatatt aaatatggca gccgcatttt
     2341 gatggtagtc agccaactgg cgtcatccgg aatgcctgta accggctgta taccacttgc
     2401 cgggaaaata tgtaatgcct ctacattggc aaacgacatt aatagtatag tcgccaccca
     2461 aaatgttggc atagcatata aaacaaataa tatgacagaa gatgttttgt caaatggaga
     2521 attttttttc gctgctgcct taatgcctat gggtaaacta attaaataag caagtattac
     2581 agatgttaat gcaaaaaaca gcgaccattt aattcgttca cctattacat tccccaccgg
     2641 caattttgtt tcataagaat atccgaaatc gccacgaatc aatcctttgc aatcaacact
     2701 accttttccg gttaaccagt tgccatcgcc aaaaatccag cggtggtatt gattataacc
     2761 ataaaaatga atagccggta tccaggtttt tgctatttgt ttttcggaaa ttaattgctc
     2821 atatagcgct tttgttttta atatttgtgc tttaataggt tcaaaataag gatatgttgc
     2881 tgcgtaatgt gctagagtat ttaatcgaag ttgaattggt gcttcttcat aactgtacat
     2941 taacacacca atattttgtt tggcttcaat gatggtatct aacacaaaat tttttccata
     3001 aaccgcaaaa ttgctggtgt ccggaatcat tctgttgatg gctgtagata aagcatgtaa
     3061 ttggttattc caggcagcaa tattttccca gttaccgtat tgactaatca atcgttttaa
     3121 cgcagtgcgt tcattgtcgt cataaacttt atataaagta tcgggtgttg ctgcattact
     3181 taggctcaca taaaacacag gtaaatctaa acccagtttt ttgcgccagc gctctttttc
     3241 ttttgcagta ttagctgttt gtgtgctgaa ctctcctccg gattcctgag cagaaaccat
     3301 cctgtccagc gggtcaccgg gtgcattaat actgataatg aacgccaata acgaaataat
     3361 tatcagcgtt ggaataaaaa acaatatgcg tttgagaaga tatttgccca ttacctgagt
     3421 tcaattgcct tgtaaaaata cagtaagggc ggaattattt cccaactaat gattttattg
     3481 atttgattcc gttgcatttg ttgccgtgcc ggactgagca ccatataaag caatcaatcg
     3541 caagtggttt aatatattcc ccggacgatc aaatgtcatt acctgattac cgaaacgttt
     3601 atgaataata tttttccgat acgctgaata caagaaaata taaggttgtt catcgtaaat
     3661 aatttgttgc attttttgcg acaatggaat acgtttttct gcgtccatgg taacctttat
     3721 ctggtcgatt aaggcatcgg tttcactatt gccaaatcca acataattac tacctccggt
     3781 tgaatatgat ttagtgtgcc acaattgtgt aaactcttcg ggtaatgaac cacctgccca
     3841 ggcactcata tacatatcga aatcgtgttt ttgtaaatgc tccttaagtg tgtttccttc
     3901 aatcggaatt aaaattaatt caataccacc tttgtaaact gcttctttaa tcatgatggc
     3961 agtttcttca acaaatgttt gcccggcctg atatttaaat tcaatttgca tgttggtttt
     4021 tacaccatca atcactttat ctctgatatt atcaccatca ctgtctttcc atccggcttc
     4081 atctaataat tttgtcgccg ctgcaatgtc gtttggaagt ggcttcagat tagtattata
     4141 ttcatccttt aaaggtgaaa tcggacctgc catccgctta gcacggccta atgtaataac
     4201 gttaatcagg tcgtcaacag gcactaaata agccattgct ctgcgcacct taacatcgtc
     4261 aaaaaacttt ttgtgcgtta caccatcagg tttcatattt aaaccaatat agttgtaact
     4321 atagttgtcg gtgaattcaa tattataatg cgttaaaaaa tttccaccat ccattaattc
     4381 tgttgcagtt accgttgata accacaccga agcatctaaa gtttgtgaac caaattccag
     4441 tttttgagct gttgggtctt tgattatttt aaatattatt ttttcaggga aactggtttc
     4501 atattcgtta gggttgctta atttttgagt ccagtgattt tttttgcgtt caagaattaa
     4561 attttgtccg cgtgtccatt ctgcaacttt ataggcaccg gaataattta ataaactgat
     4621 atcgttacct aatgccgcat cattaaattt atttgcccat gctaataaat ctttttcatt
     4681 atcagtgtta aaatccggat tgttaaactg agcaaatgtg tatttcgcca aaacattgtt
     4741 cgggtcataa tatttgcgtt gaaataccgg atagtcgctt aagaaattaa tattatttat
     4801 ttcctcctgc ttcattacta tcgtgaattt atacgcatta tcaggataag taattacatc
     4861 ctgaatgttt tcaaaatatg atttagtaat cgggttattg gtaagcggac aacggtttgc
     4921 ttttaacata aatacacaat cttcggctgt aatagcactg ccatcgtccc atgtagcatt
     4981 tttgcgcaag gtataagtat aacgcaaatt gtcatccgaa acttcaggca tagcttctgc
     5041 aagatcgggg caaacattta aattgattac atcgttgcgc agaatataat tcgcagttaa
     5101 ttgcaatatc cagctgcgtg atgctgtgcc accgtttgtt ggatgtaaat tatcaggatc
     5161 acccggccaa tggttcacta caatgttttc tttgctccat tccggcaata tatttgttgc
     5221 atcagcaatg cgtttattcc gaattttatc tgaaacgtta agggttgaaa tgtcgttctt
     5281 gttgttaccg ccatttcctc cgcatgaagc aagtaaaaag gctgttgcaa tagtcagggt
     5341 cgaaaggaca ggaaacttag tgctcataat ggtagtgctt tagtcaccaa agtaaatgaa
     5401 taatattgat ttttttaaac tgtatgaata gcagattgtt atattaactt taactcaata
     5461 ttgacaggtg tatgaaaaag gttttaatca gcggaggttc agggttaatc ggcaggcatt
     5521 taacgggtat tttattacaa aatggttatg aagttgcctg gctcagcaga agtgttaagc
     5581 cacttaagga tgttaaaatg tataagtggg atgttgatgc cggttatata gaccctgagg
     5641 caattctttg ggccgatgtt ttggtccatc tggcagggga gtccattgca ggaaaacgct
     5701 ggaccgcaaa aagaaaacag gagattattg aaagcaggac aaaaagtacg gaactgctta
     5761 ttaccgcgat gcagcaacac aaccatcata ttgggcaggt ggttgcagct tcggcaattg
     5821 gattttatgg tgaaacggga gacttgcctg ttgatgaaac aaatgcttcg ggcaatggct
     5881 ttttatcgga atctgttagg caatgggaaa atgcaacaac aaagttttca gctttaggta
     5941 ttaaccttgt tacgctgcgc atcggcattg tgttggccga aaatggtggt gcgttaaaag
     6001 aaattgtcca gacaaaatcg ctgcgggtat tacctattat gggttcgggt aaacaaatat
     6061 atgcatggat acacattcag gatgttggtc aaattatgtt gtatagtatt caacaaaaat
     6121 taacaggcgt atataatgca gttgcgccac atccggtttc attaaaatca atcatccaac
     6181 attataagca agtgagtgga aaatggtatt tacaaatgcc tgttccagcc tttgcgttaa
     6241 aacttgtttt aggtgaatta agtgcagttg tgctcaccgg tcaaaatgtt agttgtgcaa
     6301 aaattcaatc tgcaggatat cagtttagtt ttcctgatat tacatctgca ttaactcaaa
     6361 taattacaac ataaaaaagg ccggtgcact cctcagtaac accggccggc aaccacatat
     6421 tgataattat tttttagcta aaccgttaaa tgtttttacc agagaaataa attctgctct
     6481 gtatccattc gcatctgctc ctgttgcaga agaagcgagt ttcaatactt catcataact
     6541 ggcatcacct ttaaattctg aatcgcgaag taacatacca aactcaacaa cagcactggc
     6601 aaaacggaaa ttatcggaag tgcttgcaaa tggaacggtt gttttaaaaa gcgacttctc
     6661 aataagttta cttgtttcac tgttaggtgc tttataacgc aattttaaag tcataatttc
     6721 atcggtatta taagcatcgt ttttaatgga agtggtttga tattttaaat cttccacagt
     6781 gcctgaagtg ctgtttatac ccgatggctg ataggcagat ttacgcaatt ttaattcata
     6841 taaagcagtc actttatgtc cggcacccaa ttcacctgca tctttagtat catcatcaaa
     6901 atcttctttt gccaatattc tgttttcata accaattaat cgatacgatt caactaaagc
     6961 aggattaaat tcaatttgta ttttaacatc ttttgcaatg gtaaataatg tgccacgcat
     7021 atcggtagta aatacttttt tggcttcttt ttcactgtca atataataat acgcaccgtt
     7081 gccattgtcg gcaatttttt ccattttact gtccttataa ttccccattc caaaaccaca
     7141 aatactgata aatacacctg tttttctttt ttcttcaatc agtcttgtca tctctgcatc
     7201 gctactggca cctacattaa agtcgccatc ggtgcaaaga attactctat tatttcctcc
     7261 gggaattaaa ttctttacag caatttcata cgcaagttta atacccgcac cgccagctgt
     7321 ggaaccacct gcctgtaaat tattcagtgc gtttttaatt gttgatttct gattacatgg
     7381 ggtagaaggt aatacctctc cggcagcacc tgcataaaca accaatgcca cacggtcttt
     7441 ttcatttaaa ttatcaacta aggtattgag agaactaatt actaaaggca atttattttg
     7501 atcactcata ctaccactta catcaattaa aaataccaga ttagaggcag cagcttcgct
     7561 gtaatcaata tcttttccct gtaatccaat cattactaat ttgctttcgg tattccatgg
     7621 gcattcactc atttcgtagt taattgcaaa tgggtcatta ccttttggtg cggcataagt
     7681 gtaatcaaaa taatttaaca tttcttcaat acgcactgca tcttttggag gtgtttgacc
     7741 gtaagtaata aacctgcgta tattggcata agcagcattg tctacatcaa tcgaaaatgt
     7801 agataaagga ttgcccacaa ctgtcttaaa tgcattttca ttaattacat cataggcttc
     7861 agtattatgc actacttcac ccccatgaaa ttcttcatct attgaaagac tcatatagct
     7921 gatagcatca aatgacacac tgttactttg agcaaccacc ttgcaagcaa cttcaccttt
     7981 tttgtcttct acattttctt ttatttgtaa aacctgaagt gaatcgcctg atacacttac
     8041 ttgcatagcc atgctgatat ttatttcttt agattcacca tcagcaattt caatagggat
     8101 tttttgagcg acataaccgg ttgccgacac ttgtaaagtg tatttacctg ctgctaattt
     8161 ttcaatacta tataaaccgt tttcatcagg atgaaatttt gttttgttat ctaaagtaag
     8221 ttcggcactt accaatggtg ttccgtaagt ggattctaca atcaatccaa atattttaga
     8281 tgttccggta aaatctactt ccggtgtgcg attagattca gcacctatta aaatgcctga
     8341 tgctatcagc accacgagca gcgaaggaaa aagtaacttt tgcataagat atttatttag
     8401 tgttagaaat gaaataccca catgtgatgc aaaagcagga gttaatccat aaatggggtc
     8461 aaattaattt ggttgagaaa taactttaaa tagacaaaga cctgccaggt ttttaaaacc
     8521 tggcaggtct tatgttaaaa atatagggac aaaaaaaaga ggatatctat aaaagacatc
     8581 ctctttgtaa aaagtttaat gctattactc aaccctgctt gaacccaggt cgatgtaaac
     8641 taattctgaa ccggcatctt tggctttgcg aacagtgtcg ataccatctt ttacatggat
     8701 atgcaccttt aaatcatcgc tgcctttagc caaaataaaa acagggtcgc caacaatatc
     8761 ttcaatgtaa actttgaaga caccttcgcg gtcatacaca tactggttgt ttcctgtttc
     8821 atctttataa ataacatcaa attgagttcc ggtaacaatg taagtaatct tattagtttc
     8881 cggcagctca tcaacattat aacacgacga taacatcatg ctaatggcta ataaaaatac
     8941 agttagagtc ttcatactta aaacgttagt ggataaaggt aaaaataaac ttctcatact
     9001 catctgtaca aaaataggga atatgtgaca tggttatgtg gataaagtga ccttttgtaa
     9061 gaaaacacga ttaatcggct aaaagtcgca attttgctgc ttcaaagcct tttattattt
     9121 cctgcaaaat ggttgctgca ggcaagatat cccgaatggt ggcacttacc tgtccaattt
     9181 ctagttcacc ttcggctaaa tcgccttcga acatcccttt ttttgccctt ccgcggccca
     9241 ataaagtttt gagttcgtcg ggcccggcac agcgctggta ggcggcttca acctcctgat
     9301 agaatttatt tttgattaat cgaaccgggg ttatttcttt taaggtaagg gtagtgcttc
     9361 cctcgtcagt ttgaataatt tcctgcttaa aattgatatg tgcactgctt tcaatactgg
     9421 ctgcaaaacg gctgccaacc tgcactccgt ctgcccccag ggccatacaa gccagcatcg
     9481 ctctgccatc gccaatacct cctgcagcaa ttacgggaat tgtgacagcg tcacgaaccg
     9541 c
//
LOCUS       DAORKW010000075         9504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_173_length_9504_cov_6.167743, whole genome shotgun sequence.
ACCESSION   DAORKW010000075 DAORKW010000000
VERSION     DAORKW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173_length_9504_cov_6.167743"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1123)
                     /locus_tag="PLB46_04710"
     CDS             complement(95..1123)
                     /locus_tag="PLB46_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF75850.1"
                     /translation="MNRIVLSLFCALIAISSSAQDIHFSQYSQSPLTLNPALTGLTPC
                     TYRGVLNYRNQWASAVGPASYQTFAGSFDAGLWRNKLDGNIVGVGAMIYNDISGDGAL
                     TNMTIMGSFAYHQKLGDENHYLSVGGQLGLVQKRVDYGKLIFESQIGNNGVDPTLPSG
                     EYGDDNFSYLDINAGANWRSRFSNKFAMQIGGAYFHLSEPTESFYNANDNKINAHYLG
                     YGSFKIGIGKNTVLIPSYLFMQQTQGSNVQMNTGADVGFQLEKGVSMAYVGAHYRSTD
                     DIDVADAVIINLGLDYNNINFGLSYDVNISGLSAVTNYRGGIEFSLVYWGCLDVNPKQ
                     KPIDCPRF"
     gene            complement(1293..3821)
                     /locus_tag="PLB46_04715"
     CDS             complement(1293..3821)
                     /locus_tag="PLB46_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF75851.1"
                     /translation="MRTTIVLIVLFICSIAKLAAQAGDKPNFIFIVIDDLNDYIEGYT
                     DQPQLTTPNIKAIAEQGTLFLNSYSNAAGCAPSRTSFFSGKDLAYTQVYNNDDYVSKF
                     RENFTAEKNNELVYSLPEILKDSGGYFTYGINKLFHNPSSNDFDKPDSVALCEKKLSW
                     NNMVFFEDEDSTLEMLSEYAFGNYFDWGMIPDSLETILEDYKAADSAIAFIESIADGT
                     ANTCGNPFFLGLGLYRPHSERYIPQKYFPADYVNDIYAEPFDIPYNYPVNTFPYNGIV
                     MPPQPEPMYNDYYTLPDGGIASKMADNGGVYDQIESWIDGLTTLPVIDELLTDADREA
                     ILFETAKANYQMNYIAAVQYIDAQIGRVMDALNAHPEIKENTIIVLLSDNGYSLGEKR
                     HWTKWTLWEPDTRVPLLIADPTKPGNRVVRQTVTLVDIFPTVCDLAEVQYPTKPDGSA
                     YLDGLSLIDLLDGPELQFEYPALTSYKKNIGNGSCFPHHSVRNDRWHYIRYRQNNDGA
                     FGVSYCDSTNVSFEEELYEIGVNKETDPYEWNNLISNPDYIPLVHYLNQWMPDSALYM
                     DRTFKLLMSSVSPDCFAAHDDTLLLTFTIYDTTGILISPPADYIYKWSNNITDDVMYG
                     ETISFDLQLLSDAEYAANERIVFYIEMLNPDNDIVAGFDLQYYFLNGANIPNATFNVI
                     NLSGLTVQVIDYAITGSYLDTWWDFGDGNIIDETIPAPYTYDAPGTYLITNYANYGND
                     SCVISFTQNITLDTNITQPEDLVQVFPNPANNNINVLFPMVTEFASVTIYDLAGQEIL
                     TNRYLNGNYGFTCKIDISDFKQGMYLLNLQTAEVNSTTPFVVVR"
     gene            complement(3824..6346)
                     /locus_tag="PLB46_04720"
     CDS             complement(3824..6346)
                     /locus_tag="PLB46_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF75852.1"
                     /translation="MKKTILLLFTLLQAALQAQDVAKPNILFIVVDDLNDYIEGFEGH
                     PQIQTPNIKSIADNGYLFTNAFCNAPVCGPSRTSFLSGKDLLYTQVYDNNNYLDEFRD
                     NFTEANNNEEVITLPEHLKDAGGYYTISINKVFHDAFNKDFDNVIADPCLKTLSWSRA
                     TSFNDFAWLLTEMEIYNSGLQEMNWGILPDSLEEDLKDFRAVDTAIKIFKDIVDGDLA
                     LCDSAFFMSIGFDLPHLDLYVPEKYFPADYIKDFYADTFNIPYNYPADTFPTNGIIMP
                     PQPEIRWNDYNNLGPLGKAISAGQADIEASLQLFCDTLDPMPEIDPGLSDSARAEILY
                     ESTRANAIMAYMAGIQFIDAQVGRLLEYLNSEPELKANTIIIFAGDNGFSFGEKHHWF
                     KRSLWEPDVRVPFIIYDPNRGSNNICDQTVSLLDLYPTICELTGTPFPTFEDGSNYLD
                     GKSIVPILDNPDNNWERPVLISFESEDNKEGSCFPQYAVRSEKFKYIRYASDGGDPYD
                     ECDADSSYYEEELYEIGKYREVDPNEWNNLITNDDYDDVVTYLQQWLPDGPLYLDATY
                     ALRISDYSDPCLYGYEDSILLHFDLFDTLGNSISPLPGFNYVWSLNLSDEIFNGNDIL
                     LPLNVIDAATFNANSEILVYAKMVDTINNIVYALDIRTFQINALTQPTVSFNTVQIDD
                     YTIQILDFSLTGVYSDIWWDMGDGTILHTLNPGIYTYDSSGAFNITCYVQYGNESACL
                     ISYTRSIISGVEQFEGKGNIIIYPNPASDLISIFTNVYDTAGVVYITDALGNLVYQKT
                     TTAADYPFMQISTQFLPSGVYFLVLKQEDFQETGIFVVAQHN"
     gene            complement(6372..8888)
                     /locus_tag="PLB46_04725"
     CDS             complement(6372..8888)
                     /locus_tag="PLB46_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF75853.1"
                     /translation="MKRTQLLLFLIMLWIGPGFGQAGSSPNIILIYIDDMNDYGMPVN
                     GLPGVEYPGMQQLISQGTTFYNAHASSPKCGPSRTSMLAGKDVSYTKIYNNPTCKPFT
                     DYFTPGLDNEVFFTLPGYLKSNGYYTYGINKIFHCFDSEPDYDTITRNPCSKSLSWNK
                     YSWFYENEDANILTYGNAHNIGMHDMATAILPDSLEHLLYDYRAVDSALLFIDGVVNG
                     EVSTCGNPFFMSIGIRKPHSPWYVPEKYFSSDFVTNLYTQPFDYPYNTPDNSYPYNGV
                     VLAPQPDTIFNDYNNLGALGKVMADYDSAIIHYASEIEDYDPVPQFADGLSEAERLDI
                     LTKSLFANTQLGYLAACHFADAQIKRFLDSLSLYPEVYHNTVVVLVSDNGFSLGEKQH
                     WKKGTMWENDLRVPFIITDFRNPVEQQVFSAVSLLDVFPTICDIASIQKPLQPDGGVY
                     LDGHSLFPFLSNPNIKIEKPAIATYREQNLSQCGCFPQFSIRNNNFHFLYYTSNGADL
                     TLPCNASGAWHESELYEIGNNFKQDPHEWNNLIGNEDFKPVINYLQQWLPDSSLYLQK
                     AYFLEINDVSPNCYANNDETLHLSFSIFDTSGSLVTPPETFLYRWTNNLTGDTLYGSE
                     VDFSTSLIDEVTFSSAKEMLVFVEMLDTSKQTIVGLALRHVHINSENMPVVNFNLKSW
                     GFQTVKVEDFSITGDYNNYWWDFGFGPTFLNTIPGPVIFEEEGPYTIKCIIEYGNNLS
                     CENVIEKTYVTEIGIFDNNSPLVVAPNPTNAQLTISIPDNTTGGNIFIYTSGGSLVKQ
                     FALGAQINNFLISTNDLPQGCYFIKIFTPNTNYSATFIKN"
     gene            complement(8891..>9504)
                     /locus_tag="PLB46_04730"
     CDS             complement(8891..>9504)
                     /locus_tag="PLB46_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75854.1"
                     /translation="YNNGDPLYIYFSLIANGTKITMGFDLQVFSVNPEKTPEIHYDLV
                     SNDKGESFVSNFSIQPPANSWWWEINGDSLFYNTLPGPVNAGSNNTVTYKCIASYGNN
                     NCELVAEKTVVTKPLTYYDEHQLICLPNPTKSEVILMHTDNVIYGNLRIYDISGTVVK
                     EMNISDAFSSFYKINISDLSAGLYVITLTDKEKVLTTSLIVTN"
BASE COUNT         3116 a         1769 c         1582 g         3037 t
ORIGIN      
        1 atccatttac gaaaaacacc aaatttgaat tttacccggc ataaaaaaag cggatagttt
       61 ttcaaccatc cgctctaata gtatttatat tattttagaa tcgagggcag tcaattggtt
      121 tttgcttagg attgacatct aaacatcccc aatacaccaa tgaaaattca ataccgccac
      181 gataatttgt aacagcagat aatcctgaaa tattaacatc ataactcaat ccgaaattga
      241 tgttattgta atcaagtccc aggttaataa ttacagcatc agcaacatca atatcatctg
      301 tagagcgata gtgtgcacct acataagcca ttgaaacacc tttttcaagc tggaatccaa
      361 catcggcacc agtattcatt tgtacgtttg aaccttgagt ttgttgcatg aataaatatg
      421 aaggtattaa cacagtgttt ttgcctattc caattttgaa tgaaccataa cctaaataat
      481 gtgcattgat tttattatcg ttggcattat aaaaagattc ggttggttca cttaaatgga
      541 aataagcacc accaatttgc atggcgaatt tatttgaaaa acgacttctc cagttagcac
      601 ctgcattaat atctaaataa gaaaaattgt catctccata ttcaccactt ggcaaagttg
      661 gatcaacacc attattccca atttgtgatt caaaaattaa tttgccgtaa tcaacacgtt
      721 tttgaactaa acccaattgc ccaccaactg ataaataatg gttttcatca cctaattttt
      781 gatgatatgc aaatgaaccc atgatggtca tattggttaa agcaccatct ccggaaatat
      841 cattgtaaat cattgctcca actcctacaa tattgccatc caacttattt ctccataaac
      901 cggcatcaaa agaaccggca aaagtttgat aggaagccgg accaacagca cttgcccatt
      961 ggttgcggta attgagcacg ccccggtaag tacaaggtgt taaccccgta agcgccggat
     1021 tgagcgtaag tggtgactga gaatattgcg aaaagtgaat atcctgagcg gaggagctta
     1081 ttgcgattag tgcgcaaaaa aggcttaaga ctattctatt catactgttc atcgtggatt
     1141 atccttccaa agttaataaa atcctgtatt ccacacaaaa ttaacaagag tatttgtcat
     1201 attggagtaa aggtttaccg gacgaatcag ggtcgaagtg cccaaaaagt gggctaaacc
     1261 ctttaaaaac aacgacttat acaccaaaat ccttacctaa ccaccacaaa aggggtagta
     1321 gaatttactt ctgcggtctg caaatttaac agatacatac cttgtttgaa gtccgaaata
     1381 tcaattttac aggtaaaacc atagtttcca ttcaggtagc ggttggttaa aatttcctga
     1441 ccggctaaat catatattgt tacagaggca aattctgtaa ccattggaaa caatacattg
     1501 atattattat tcgcaggatt tgggaaaact tgcaccaaat cttcgggctg ggtaatgttc
     1561 gtatctaaag taatattttg cgtaaatgaa atcacacaac tatcattgcc ataattagca
     1621 taattggtaa ttaaatatgt tccgggtgca tcataggtat atggagctgg aattgtttca
     1681 tcaataatat tcccatcacc aaaatcccac caggtatcaa gataacttcc ggtaattgca
     1741 taatctataa cctgaacagt aagtcctgat aaattaatta cattaaaagt agcatttgga
     1801 atattggctc cgtttaaaaa ataatattgt aaatcaaatc cagcaacaat atcattgtcg
     1861 ggatttagca tttcgatata aaatacaatt cgttcatttg ctgcatattc tgcatcactt
     1921 aatagttgta aatcaaatga tattgtttca ccgtacatca catcgtcagt aatattattc
     1981 gaccatttat agatataatc tgccggcgga gatattagta ttcctgtagt atcataaatc
     2041 gtaaacgtta ataataaagt atcatcatgt gcagcaaaac aatcaggtga aacagagctc
     2101 ataagcagct taaatgttct atccatataa agcgccgaat cgggcatcca ttgattgaga
     2161 taatgcacaa gcggaatata gtcagggttg ctgattaaat tattccattc ataaggatct
     2221 gtttctttgt taacaccaat ttcatataat tcctcttcaa aagaaacatt ggtagaatcg
     2281 caataactaa caccaaaggc accatcatta ttttggcgat agcggatata atgccatcta
     2341 tcgttacgca ccgagtgatg cggaaaacaa gaaccatttc ctatattttt tttatatgaa
     2401 gtcagggctg gatattcaaa ttgtaattcc ggtccatcta ataaatcaat tagacttaac
     2461 ccatcaagat aagcacttcc atcaggttta gtaggatatt gcacttcagc taaatcacaa
     2521 acagttggaa aaatatcaac aagtgtaacc gtttgacgca ctactctgtt gcccggttta
     2581 gtagggtcgg caattaataa aggcacacgc gtatcgggct cccataatgt ccattttgtc
     2641 caatgtcttt tttcgcctaa agaatatcca ttatcactca ataaaacaat aatcgtattt
     2701 tcttttattt caggatgagc atttaacgca tccattacac ggccaatttg tgcatctata
     2761 tattgcactg cagcaatata attcatttga taatttgctt ttgctgtttc aaataaaatg
     2821 gcttcgcggt cggcatccgt taaaagttca tcaattaccg gtaatgttgt aagaccatca
     2881 atccaggatt caatttgatc gtaaacacca ccattgtctg ccattttact tgcaattcct
     2941 ccatccggca aagtgtaata atcgttatac atcggttcgg gttgcggagg cataacaata
     3001 ccattatatg gaaaagtatt taccggataa ttataaggaa tatcgaatgg ttctgcataa
     3061 atatcattga cataatctgc aggaaaatat ttttgcggaa tatacctttc agaatgtggt
     3121 cgatataatc ctagtcctaa aaaaaatgga ttaccacaag tattagctgt gccatcggct
     3181 attgattcaa taaatgcaat agcagaatca gcagctttat aatcttcaag tatggtttcc
     3241 aacgaatcgg gaatcattcc ccaatcgaaa taattgccaa atgcgtattc tgataacatt
     3301 tctaatgtac tgtcttcatc ttcaaaaaac accatgttat tccatgataa ttttttttca
     3361 cataatgcta cgctgtcagg tttatcaaaa tcgttgctgc tggggttatg aaataattta
     3421 ttaataccat aggtaaaata tcctcctgag tcttttaaaa tttcaggaag tgaataaact
     3481 aactcgttgt ttttttcagc cgtaaaattt tctctgaatt tacttacata atcatcgtta
     3541 ttatacactt gtgtataggc taaatctttc ccgctaaaaa aactggttct tgaaggtgcg
     3601 caaccggcag cattactata actatttaaa aataatgttc cctgctccgc aatcgctttg
     3661 atatttggtg tggtaagttg tggttggtcg gtatatccct cgatgtaatc attgaggtcg
     3721 tcgattacaa taaaaataaa attaggttta tcaccagcct gtgctgctaa ttttgctata
     3781 gaacaaataa ataaaacaat taggacaatg gtggtgcgca tgtttagttg tgttgtgcta
     3841 ccacaaaaat acctgtttcc tgaaaatctt cctgttttaa cacaaggaaa tatacaccgg
     3901 aaggtaaaaa ttgagtagaa atttgcataa aaggataatc ggctgcagtt gtagtttttt
     3961 gatagactaa attgcccaaa gcatctgtaa tatatacaac accagctgta tcgtaaacat
     4021 tggtaaaaat gcttattaag tcactagcgg gatttgggta gatgattata tttcccttgc
     4081 cttcaaattg ttccactcct gaaataatgg agcgggtata acttattaaa catgcagact
     4141 cattaccata ttgcacataa caggttatgt taaaagcacc tgaactgtcg taggtatata
     4201 tgcctggatt taaagtatgt aaaattgttc catcacccat atcccaccaa atgtcggaat
     4261 atacgccggt tagcgaaaaa tctaaaattt gaatggtata atcgtcaatt tgtaccgtat
     4321 taaaacttac cgtaggttgt gttagtgcgt ttatttgaaa agtgcggata tccaaagcgt
     4381 aaacaatatt atttattgta tcaaccattt tggcataaac aagtatttca gaattagcat
     4441 taaatgttgc agcatcaatt acattaagtg gcaacaaaat atcattgcca ttaaatattt
     4501 catccgataa attaagtgac caaacataat taaatccggg tagcggtgaa atgctattgc
     4561 ccagtgtatc aaataaatcg aaatgcaata aaatgctgtc ctcatatcca tataaacaag
     4621 ggtcactata atcgctaatt cttaatgcat aagttgcatc taaatacaat ggaccatcag
     4681 gaagccattg ctgtaaatat gttactacat cgtcatagtc atcgttggtt attaaattat
     4741 tccattcatt tggatctact tcgcgatact tgccaatttc atataattct tcctcgtaat
     4801 aagaagagtc cgcatcacat tcatcataag ggtcgcctcc atcagaagca tatcggatat
     4861 atttaaattt ttcggacctg acggcatatt gaggaaaaca agaaccttct ttattatctt
     4921 cagattcaaa actaatcaat acaggtcgct cccaattgtt atcaggattg tctaaaatgg
     4981 gtacaatact tttgccatct aaataattac tgccatcttc aaaagttgga aatggtgttc
     5041 cggttaattc acaaatggtt ggatacaaat ccaataaact tactgtctgg tcacaaatat
     5101 tattacttcc gcgatttggg tcataaataa taaaaggcac tcttacatct ggttcccaca
     5161 agctgcgttt aaaccaatga tgtttttcgc caaaagaaaa tccattatcc cctgcaaaaa
     5221 ttatgatggt atttgccttg agttccggtt ccgaatttaa atactccaac aaccttccaa
     5281 cttgtgcatc tataaattga atgcctgcca tataagccat aatagcgttg gctcttgtac
     5341 tttcgtataa aatttctgct ctggcagaat cactcaatcc gggatcaatt tccggcatgg
     5401 ggtccaatgt gtcgcaaaat agttgaagac tggcttcaat atcggcctgc ccggctgaaa
     5461 ttgccttgcc aagtggccct aaattattat aatcgttcca tctaatttcc ggttgtggtg
     5521 gcataataat accattggtt ggaaatgtat ctgccggata attatatgga atgttaaacg
     5581 tatcagcata aaaatctttg atataatctg cagggaaata tttttccggt acatataaat
     5641 ccagatgggg caaatcaaaa ccaatcgaca taaaaaatgc gctatcgcac aaggctaaat
     5701 ctccatcaac aatgtcttta aaaattttga tagcggtgtc cactgctctg aaatctttta
     5761 aatcttcttc gagactgtcg ggtaaaattc cccagttcat ttcttgtaaa ccactgttat
     5821 agatttccat ttcggttagc aaccacgcaa aatcgttgaa agatgttgct ctgctccacg
     5881 acaatgtttt taagcaggga tcggcaatga cattatcaaa atctttatta aatgcatcat
     5941 gaaatacttt atttatacta atggtgtaat atccgcctgc gtcttttaaa tgctcaggca
     6001 aagtaattac ctcctcatta ttattcgctt ccgtaaaatt gtccctgaat tcatccaaat
     6061 aattgttatt atcatacact tgcgtataca ataagtcttt cccgcttaaa aagcttgtcc
     6121 tggaagggcc gcaaacgggg gcgttacaaa aagcattggt aaataaatat ccgttatcgg
     6181 ctatgctttt tatattgggc gtttgaattt gaggatgccc ttcaaaccct tcaatatagt
     6241 cgtttaaatc atctacaact ataaacagaa tgttcggttt agctacatcc tgtgcctgaa
     6301 gcgctgcttg taataaagtg aaaagaagta ggattgtttt tttcatgagt gtttgcaaat
     6361 gtaatatacc attaattttt tataaaagtt gcactatagt tagtgtttgg ggtaaatatt
     6421 ttaataaagt agcaaccttg aggtaaatcg tttgtgctta ttaaaaaatt attgatttgt
     6481 gctcctaatg caaattgttt tactaatgat ccaccacttg tatatataaa tatattgcca
     6541 cctgtagtat tatccggtat tgaaattgta agttgcgcat tggttggatt tggtgcaacc
     6601 actaaaggcg aattgttatc gaatattcct atttcggtca cgtaggtttt ttcaattacg
     6661 ttttcacaac ttaaattatt accatactcg attatacatt taatagtata aggaccttct
     6721 tcctcaaaaa taacagggcc ggggatggta ttcagaaatg taggcccaaa accaaaatcc
     6781 caccaataat tattgtaatc acctgtaata ctaaaatctt ccaccttcac agtttgaaat
     6841 ccccaacttt taagattgaa atttaccaca ggcatatttt cggaattaat atggacatgc
     6901 cgtaaggcta aaccaacaat tgtttgtttc gaagtatcta acatttctac aaacaccaac
     6961 atctctttag ccgaagaaaa agtaacttca tctattaacg atgttgagaa atcaacctcg
     7021 ctaccatata atgtatcgcc ggttaaattg tttgtccagc gataaaggaa agtttccggt
     7081 ggagtaacta atgatccact tgtatcgaaa atactaaaag ataaatggag tgtttcatca
     7141 ttattagcat aacaattagg acttacatca tttatttcca aaaaatatgc cttttgtaaa
     7201 tacaaactgc tatccggtaa ccattgttgt aaataattaa ttaccggttt aaaatcttca
     7261 ttacctatca ggttattcca ttcatgtgga tcctgcttaa aattattccc aatttcgtat
     7321 aattcacttt cgtgccaagc gccactagca ttacatggca aagttaaatc agctccattt
     7381 gatgtataat ataaaaaatg gaaattatta ttcctgatac taaattgagg aaaacaacca
     7441 cattgactta aattttgttc tcggtatgtt gcaatggccg gtttttctat tttaatattc
     7501 ggattactta aaaacggaaa taaactatgt ccatcaagat aaactccacc atcaggttga
     7561 agtggttttt gtatcgacgc gatatcacaa attgtaggga aaacatccaa taggctcaca
     7621 gcactaaata cttgttgttc aactggattt ctaaagtctg taattataaa tggaacacgt
     7681 aaatcattct cccacatggt accttttttc caatgttgtt tttcgcctaa actaaaaccg
     7741 ttgtcgctga ctaatacaac aaccgtgtta tgataaactt caggatataa ggaaagcgaa
     7801 tccaaaaatc gttttatttg agcatcagca aaatggcaag ctgctaaata acctagttgg
     7861 gtattagcaa ataaactttt agttaatata tccagtctct cagcttctga taatccatcg
     7921 gcaaactgtg gtaccggatc ataatcttca atttcagatg cgtaatgaat aatggcacta
     7981 tcgtaatcag ccataacttt ccccaaagca cctaaattat tataatcgtt aaatatggta
     8041 tccggttgag gtgctaacac tacaccattg taagggtaac tattatcggg tgtgttataa
     8101 ggataatcaa aaggttgggt atataaatta gtaacaaagt cgcttgaaaa atatttttca
     8161 ggaacatacc aaggcgaatg tggtttgcga atccctattg acataaaaaa cggattacca
     8221 caggttgata cttcaccatt aacaacaccg tcaataaata ataatgcgga gtcaacggcg
     8281 cgataatcat ataacaagtg ctctaacgaa tcaggtaaaa tggcagttgc catatcatgc
     8341 ataccaatgt tgtgtgcatt tccataagtt aaaatattgg catcttcgtt ttcataaaac
     8401 caactgtatt tattccaaga caatgattta ctgcaagggt tcctggttat ggtatcatag
     8461 tcgggttctg aatcaaaaca atggaatatc ttgttaatac catatgtgta atatccattc
     8521 gattttaaat aacccggaag tgtaaaaaat acttcgttgt ctaagccagg tgtaaagtaa
     8581 tcggtaaatg gtttacatgt aggattattg taaatttttg tataactcac atcttttcct
     8641 gcaagcatac tagtgcgtga tggcccacat tttggtgaag atgcatgagc attataaaat
     8701 gtagttcctt gtgatattaa ttgctgcatt ccgggatatt caactcccgg caatccatta
     8761 accggcatcc catagtcatt catatcatca atataaatca aaatgatatt aggagatgag
     8821 ccggcctgac caaaacctgg ccctatccaa agcatgatta aaaagagtaa tagttgtgtg
     8881 cgtttcatgt ttaatttgtt acaattaatg aggtggttaa aactttttcc ttatctgtta
     8941 gggtaattac atataagcct gcacttaaat cggaaatatt tattttataa aaagatgaaa
     9001 atgcatccga tatattcatt tcttttacaa cagttcctga aatatcatat atccttaagt
     9061 ttccatagat tacattatca gtatgcatga gaattacttc acttttagtc gggttgggca
     9121 aacaaattaa ttgatgctca tcatagtaag taagcggttt ggttacgact gttttttctg
     9181 caacgagttc gcaattatta ttgccatagc tggctataca tttatacgtt actgtattat
     9241 tgctgccggc atttaccgga cctggaagag tgttataaaa taaactgtcg ccgtttattt
     9301 cccaccacca gctgttcgca ggaggttgta tactgaaatt acttacaaaa ctttcaccct
     9361 tatcgttgct aactaaatcg taatgaattt caggtgtttt ttctgggtta acactaaaaa
     9421 cctgaaggtc aaatcccatg gtaatttttg ttccattagc aattaatgaa aaatagatat
     9481 ataatgggtc gccgttgtta taat
//
LOCUS       DAORKW010000076         9503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_174_length_9503_cov_5.292337, whole genome shotgun sequence.
ACCESSION   DAORKW010000076 DAORKW010000000
VERSION     DAORKW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174_length_9503_cov_5.292337"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLB46_04735"
     CDS             complement(<1..683)
                     /locus_tag="PLB46_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75855.1"
                     /translation="MKGNNISNFTRICLFFGAVALIVSLFVPLWRIDLDAPQYPEGLR
                     LLIYPDEIGGDVEIINGLNHYIGMDTLHSEDFLEFTILPYLIVFYALLIFSVMFIIKK
                     NWLTVVFVLFLLFGIVAMVDFWRWEYNYGHNLDPNAAIIVPGMAYQPPLIGFKQLLNF
                     GAYSIPDIGGWLFVSCGIILLFTVIYESKFYRRKFHKANSIVLLMGLGFLAAGCGPKG
                     PVPIMLNKDA"
     gene            complement(758..2749)
                     /gene="nosZ"
                     /locus_tag="PLB46_04740"
     CDS             complement(758..2749)
                     /gene="nosZ"
                     /locus_tag="PLB46_04740"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HRF75856.1"
                     /translation="MYYQQQHTRQLYTIVFVITLLSITSCKPKNVANAISGDAAAKTY
                     VAPGSHDEFYNFVSGGFSGQMSVYGLPSGRLLRVIPVFSVDPEKGWGYSEETKPMLNT
                     SHGFVPWDDLHHTELSQTKGEINGKWVFANANNTPRLARVDLKTFRTAEIIELPNSGG
                     NHSSPFGTENSEYIVAGTRFSVPADGAGGDVPIGSYKENFKGHISFVSVDQNSGEMNI
                     AFQLKTPGVNFDLARCGRAKSHGWFFFSCYNTEQANTLLEVNASQKDKDFIMAVNWKK
                     AEEYIKAGKGQKMDVRYAHNKYDEATHTATSEIETEVLVLDVAELKDICYFIPCPKSP
                     HGCDVDPTGEYIVGSGKLAALIPVFSFDKLQKAIADKAFDGEFEGIPIIKYEAALHGE
                     VKKPGLGPLHTEFDGKGNAYTSFFVSSEVVKWNIKDLTVIDRAPTFYSVGHLCIPGGN
                     TTKPTPKYLIAYNKITKDRYLPTGPELAQSAQLYDISGDKMQLILDFPTIGEPHYAQA
                     ALASIIENNGQIKIFKIEDNKHPYVTKSEGDSKVVREGNKIHVYMSSIRSHFSPDNIE
                     GVRMGDEVYFHVTNLEQDWDVPHGFAVKGADNAELLIMPGETQTLKWVPDRVGIFPMY
                     CTDFCSALHQEMQGYVRVSPAGSNVPLTFSLGKNSTTPK"
     gene            complement(2775..3254)
                     /locus_tag="PLB46_04745"
     CDS             complement(2775..3254)
                     /locus_tag="PLB46_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRF75857.1"
                     /translation="MRVFFNLSFLLLVTIIIVSCGGSGKKPANTTGDEPKSMIPDTET
                     YDPNRGTGKFTAENVTLGAIDAAMASAGEDISKTKCQSCHKLTDEKLVGPGWKGVTTR
                     RTAHWIMNFITNPDPMIDKDPALQAQLELCLVRMPNQNLNDEDARKVVEFMRQNDNQ"
     gene            complement(3362..5401)
                     /gene="uvrB"
                     /locus_tag="PLB46_04750"
     CDS             complement(3362..5401)
                     /gene="uvrB"
                     /locus_tag="PLB46_04750"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRF75858.1"
                     /translation="MEFKLTSKFTPTGDQPEAIKQLTEGVVNGEPAQVLLGVTGSGKT
                     FTIANLIQQTQKPTLILSHNKTLVAQLYGEFKQFFPENAVEYFVSYYDYYQPEAYIST
                     TDTYIEKDLAINAEVEKLRLKTTSSLQSGRRDVIVVATVSCIYGMGNPTEFKESVVRV
                     NVGDKISRNYLLHRLVDALYSRAAIDFKRGNFRVNGDVVDVFLAYADYGYRITFFGDE
                     VEEIEIFNPETGKATEKQKNIAIFPANLYIAPRDILPSIIHEIHAELDAQVTYFESIG
                     KYIEAKRLSERVNFDIEMIKELGYCSGIENYSRFFDRRKTGQRPFCLIDYFPDDYLLV
                     IDESHATIPQVGAMYGGDRARKMNLVEYGFRLPSAMDNRPQNFDEFMGLLNQTIYVSA
                     TPGDFELKQSEGVIVEQVVRPTGLLDPPIEVRPSLNQIDDLLHEVDERIKVNERVLVT
                     TLTKRMAEELSKYLMRMNVNCKYIHSEVETLERVEILRDLRLGKIDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRNTRSLTQTAGRAARNSNGKVIMYADKITESMQRTIDET
                     NRRRAKQIAYNTEHNITPTTILKSTEDIFKHNIGQGERKGEPQPYIEPQDRTALGLVA
                     DPVVHLMSKQQLEKAIVDAKKKMLQAAKDMDFLEAARLRDEMYGLEKILDGRVEG"
     gene            5517..5966
                     /locus_tag="PLB46_04755"
     CDS             5517..5966
                     /locus_tag="PLB46_04755"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HRF75859.1"
                     /translation="MRFRDITSITGMGGLFKMESHKPSGLIVTSLTEGWTKFVSNREH
                     LFSPLDNISIYTANDTVELLDVMLKAYEQKSSNPPVDAKSDNATLSAYFEKVLPEYDR
                     EKVHISDIKKFIKWFQILDEKGILATEIEEKAKEEAAAKTEETTTKE"
     gene            complement(6048..6881)
                     /locus_tag="PLB46_04760"
     CDS             complement(6048..6881)
                     /locus_tag="PLB46_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75860.1"
                     /translation="MKRFQSTHVFLCALLILIITGLSKFSYGKEDGAPSGNTGSPGDD
                     QTCAHVDCHTGSASEKDGLIATDVPASGYLVGETYLITVTIEEIGITRFGFQASPQNL
                     EGDEMGEMALIDSDQTKFTGGGKYITHTNAGTDGVDSKTWTFNWTPEEASGDVTFYVA
                     VNASDDEDDASGDHIYTSSVTITEDPDNHPLSIEEMNDILFDIHGIVQDELAVTVSTP
                     VNNPIVIDIIDIHGRLVLSNKHQYANGTFVMPVDNLNKGMYFVRISNAQGSLTKQFFK
                     N"
     gene            complement(6979..7452)
                     /gene="bcp"
                     /locus_tag="PLB46_04765"
     CDS             complement(6979..7452)
                     /gene="bcp"
                     /locus_tag="PLB46_04765"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRF75861.1"
                     /translation="MAELLSITHLKPGDKAPAFNALDQDGNKVSSTGLKGQRFALYFY
                     PNDDTETCTKQACNLRDNYAALKKAGIKIIGVSHAPVESKKKFADKYKLPFTLLADTD
                     LTIVKKYGVYGEKLFMGRTITTIHRITFLINEKGVIEKIIHKVWAGKAASQILET"
     gene            complement(7460..8167)
                     /locus_tag="PLB46_04770"
     CDS             complement(7460..8167)
                     /locus_tag="PLB46_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRF75862.1"
                     /translation="MAEKYRKKKLFKVKDWTELSAQSSWRMFKIMAEFVDGFETMDKI
                     GPCVSIYGSARTKPDHKYYKLSEQIAAKLVHEGFGIITGGGPGIMEAGNKGAYKEGGK
                     SVGLNIELPYEQSHNPYIDRDKLINFKYFFVRKVMLVKYAQAFIFMPGGFGTLDEMFE
                     ALTLIQTHKIERVPVILVGKSYWKGLMAWVRETMLHKEHNISEEDLDLFYMTDDPEEV
                     VKHIAAFYATSHLRPNF"
     gene            complement(8172..>9503)
                     /locus_tag="PLB46_04775"
     CDS             complement(8172..>9503)
                     /locus_tag="PLB46_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRF75863.1"
                     /translation="AFGLIGDSLSGVTFVSVPGNVATQEFGYMQIVIGYLLGYFVIAT
                     VLLPLYYRLNLTSIYTYLGQRFGKNAQKTGSFYFIVSRLVGAAFRLYVSATVLQLFIF
                     QPWGVPFEVTVSIVIILILLYTYRGGIKTLVWTDTLQSSFLLMGVILSIIAIAGALNL
                     DFNGIIDTVRNSEHNQIFFWDVKAPNYFWKQFISGALIAIVMTGLDQNMMQKNLSMRT
                     LPEAQKNILWFSVVLIFINLLFVSLGALLYEYIGVMGIDSPARTDHLFPILALDYLGI
                     FAAVVFILGIAAATFSSADSVLTTLTTSFCIDILGWDVTQTESTKQKQNRHIIHVLFA
                     VILLIVIIVFNAINNDAVINQVFAVAGYTYGPLLGLFAFGIITKRNIMDKVVLLVCLL
                     PPFICYYLNMHSKALFNGYQIGYELLLINGILTFIGLLLISSSNSNKLVQS"
BASE COUNT         3015 a         1934 c         1524 g         3030 t
ORIGIN      
        1 gcatccttat ttaacataat tggaacaggg ccttttggtc cacagcctgc cgccaaaaat
       61 cctaaaccca taagcaatac tatactattt gccttgtgga attttctgcg ataaaattta
      121 gattcataga ttactgtaaa taataaaata ataccacaac tcacgaataa ccatccccca
      181 atatcaggaa tcgaatatgc accaaaattt aatagttgtt taaacccaat aagtggtggt
      241 tgatatgcca tacccggaac tattattgct gcatttggat ctaggttgtg tccataatta
      301 tactcccacc gccaaaaatc aaccattgct acaattccaa acaaaaggaa taaaacaaaa
      361 accacagtga gccagttttt ctttataata aacattacag aaaaaattaa tagtgcataa
      421 aatacaatta ggtatggcag tatagtaaat tctaaaaagt cttcactatg caacgtatcc
      481 atacctatat aatgatttaa gccatttatt atttccacat ctccacctat ctcatctgga
      541 taaataagta accgcaatcc ttccggatat tgaggggcat ctaggtcaat gcgccataac
      601 ggaacaaaaa gtgaaacaat taatgcaaca gctccgaaaa acaagcatat tctcgtaaaa
      661 ttcgaaatgt tatttccttt cattattata atttttgcac taaaaaaggg gtaggataca
      721 cccatgtgta accaacccct ttactgaata atttgtatta tttcggtgtg gtagaatttt
      781 tacctaaact aaatgttagc ggcacattac ttccagcagg agaaacccta acataacctt
      841 gcatttcttg atgtaaggca ctacagaagt ctgtacaata cattggaaaa attccaaccc
      901 tgtctggaac ccatttgagt gtctgagttt ccccgggcat aattaaaagc tcagcattat
      961 cagctccttt aactgcaaaa ccatgaggaa catcccaatc ctgttccaaa tttgtcacat
     1021 gaaaataaac ctcatcaccc atcctaacac cttcaatatt atcaggagaa aagtgagatc
     1081 ttatagaact catataaaca tgaattttat tgccctcacg aactactttc gaatcaccct
     1141 ctgatttggt aacataaggg tgcttgttgt cctcaatttt aaaaatttta atctggccat
     1201 tattttctat aatacttgcc agtgcagctt gcgcataatg tggttcacct atggtaggaa
     1261 aatctaaaat aagttgcatt ttatcaccac ttatatcata aagttgagca ctttgcgcta
     1321 attccggtcc ggttggtaaa tacctgtctt ttgtaatttt attgtaggca atcaaatatt
     1381 taggtgttgg ctttgttgta ttaccaccag gaatacataa gtgtccaacc gaataaaatg
     1441 ttggtgccct gtcaattacg gttaaatctt ttatattcca ttttactact tcagaagata
     1501 caaaaaacga tgtgtaggca ttgcctttcc cgtcgaattc tgtgtgcaga ggacccaaac
     1561 ctggtttctt aacttcacca tgtaaagctg cttcatattt aataatcgga attccttcga
     1621 actcaccatc aaatgcttta tctgcaatag ccttttgtaa tttatcgaaa ctaaaaacag
     1681 gtattaatgc agctagttta ccacttccaa ctatatactc tccagtagga tccacatcac
     1741 aaccgtgagg tgattttggg catggaataa aataacagat atcttttaac tccgctacat
     1801 ccaaaacaag cacttctgtt tctatttctg aagttgcagt atgcgttgct tcatcatatt
     1861 tattatgagc ataacgtaca tccattttct gcccctttcc tgcttttata tattcttcag
     1921 cttttttcca atttactgcc ataataaaat ctttgtcttt ttgagaagca ttaacttcca
     1981 gcaaagtatt cgcttgttca gtattgtaac aactaaagaa aaaccatcca tgagattttg
     2041 ctcttccaca acgcgctaaa tcaaaattta ctccaggtgt ttttaattga aatgcaatat
     2101 tcatttcacc tgaattttga tctacactta caaaactgat atggccttta aagttttctt
     2161 tataactacc aattggcacg tcgccacctg caccatcagc cggtacacta aagcgtgttc
     2221 ctgcaactat atattctgaa ttctcagtac cgaaaggcga actatggtta cctccactat
     2281 taggcaattc aataatttct gccgttctaa atgttttcaa gtcaacccga gctaatcgcg
     2341 gcgtattatt agcattggca aaaacccatt tgccgttaat ttcacctttt gtttgcgata
     2401 attcagtgtg atgtaaatca tcccacggaa caaacccatg cgaagtattg agcattggct
     2461 ttgtttcctc actataaccc cacccctttt caggatcaac tgaaaaaaca ggtattactc
     2521 ttaatagcct gccgcttggt aatccataaa cagacatttg cccactaaag ccaccagaaa
     2581 caaaattata gaattcgtcg tgcgaaccag gtgcaacata tgttttagcc gcagcatcgc
     2641 cacttattgc attagctaca ttttttggct tacaagaggt tatacttaaa agtgtaatta
     2701 caaatacaat cgtatacaat tgtcttgtgt gttgttgttg gtaatacata tccaaaaaat
     2761 taaattttaa aaaattactg attatcattt tggcgcataa attcaactac ttttcgcgca
     2821 tcttcatcat tcaaattttg attgggcata cgcactaagc aaagttctaa ttgtgcctga
     2881 agcgctggat ctttatcaat cattggatct ggattagtta taaaattcat tatccaatga
     2941 gcagtgcgcc ttgttgttac acctttccaa cctggcccta ctaatttttc atccgtaagt
     3001 ttgtggcacg attggcattt tgtttttgaa atatcctcac ctgccgatgc cattgcagca
     3061 tcaattgctc caagtgtcac attttcagca gtaaactttc ctgttccacg atttgggtca
     3121 taagtttctg tatcaggaat catacttttg ggttcatcgc ccgtggtatt agcaggtttt
     3181 tttcctgatc ccccgcaaga aacaataata attgtaacta ataataagaa tgacaaattg
     3241 aaaaatactc tcataatgat aaattttatg agagcgaagg tattgtggac tattttagcc
     3301 gcttatgatt agagtcataa gcaaatccgg taaatagttg aaacttattt caatgtaatg
     3361 tttacccttc taccctaccg tccaatattt tttctaatcc atacatttca tctcgtaagc
     3421 gagcagcttc taagaagtcc atatcttttg cggcctgcaa catttttttc ttggcatcaa
     3481 caatggcttt ttctaattgt tgtttactca ttaaatgtac aaccgggtca gccactaatc
     3541 ctaatgcagt tctgtcctga ggttcgatat atggttgtgg ttcacctttg cgttcgcctt
     3601 gaccaatatt gtgtttgaag atatcttctg tagattttaa aatagttgtt ggtgtgatat
     3661 tatgttcggt gttataagca atttgttttg ctcttcttct atttgtttca tcaatagtac
     3721 gctgcatgct ttcagtaatt ttatctgcat acataattac ttttccattt gaattacgag
     3781 cagcgcgtcc cgcagtttgc gttaaacttc tggtatttct taaaaaccct tctttatctg
     3841 catctaatat ggctaccaat gaaacctccg gtaaatctaa accctcacgc aataaattta
     3901 caccgactaa tacatcaatt tttcctaaac gtaaatcgcg caaaatttca acacgttcca
     3961 acgtttcaac ttcactgtga atgtatttac aatttacatt catacgcatt aaatatttcg
     4021 aaagctcttc cgccattcgt ttggttaatg tggtaaccaa tacacgttca tttactttta
     4081 ttctttcatc tacttcgtgt aataaatcat caatttgatt taatgaaggg cgcacctcaa
     4141 ttggcgggtc gagtaatcct gtagggcgta ctacctgttc cacaataacg ccttcacttt
     4201 gttttaattc gaaatcaccg ggtgttgcac ttacataaat agtttgattt aacaagccca
     4261 taaattcatc gaaattttgt gggcgattgt ccatcgcact tggcaaacga aaaccatatt
     4321 ctacgagatt catttttctc gcgcgatcac caccatacat agctccaact tgcggaatag
     4381 tagcgtgact ttcatcaatt accaataaat aatcatcggg aaaataatca attaaacaga
     4441 atgggcgctg accggttttt cttctgtcaa aaaaacggga gtaattttca atgccggaac
     4501 aatatcccaa ttcctttatc atttcaatat caaagttcac acgttcactt aaacgttttg
     4561 cttcaatata ttttccgata ctttcaaaat aggtaacctg cgcatctaat tcagcatgta
     4621 tttcatgaat aattgaaggt aatatatctc tgggagcgat gtataaattg gcaggaaaaa
     4681 tggcgatgtt tttttgtttt tcagttgctt ttcctgtttc gggattaaat atttcaattt
     4741 cttcaacttc atctccaaaa aacgtaatgc gataaccata atcggcatac gctaaaaaaa
     4801 catccaccac atctccattc actcgaaaat ttccgcgttt aaaatcaatt gccgctcgtg
     4861 aatacaaagc atcaaccaaa cgatgtaata aataattgcg tgaaatttta tcccctacat
     4921 ttaccctcac cacactttct ttaaattcgg ttggatttcc cataccataa atacacgaca
     4981 ctgttgccac aacaataaca tctcgccttc cgctttgtaa acttgaagta gtttttaaac
     5041 gcaatttttc aacttccgca tttatggcta aatctttttc tatataggta tctgttgtgg
     5101 agatgtaagc ttcaggctga taatagtcgt agtaactcac aaaatattcc acggcatttt
     5161 caggaaaaaa ttgcttaaac tcaccataca attgcgcaac caatgttttg ttatgcgaca
     5221 aaataagggt tggcttttga gtttgttgta ttaaattggc tatggtaaac gttttaccac
     5281 tgcctgtcac tcctaataaa acctgcgccg gctcaccatt aaccacacct tcggttaatt
     5341 gtttgatagc ctccggctga tccccggttg gggtaaattt gctcgttagt ttaaattcca
     5401 taaagtattg ataaaaaaca cttttgaacc tcaaatataa gcagtgaatt tcaccgcctt
     5461 gtgtaaaaac aagtaaatct tgaaatcgtt tccttaaatt tgcacaaaat taaaatatgc
     5521 gtttcagaga tattacatca ataacaggaa tgggcggctt gttcaaaatg gaatcacaca
     5581 agccatcagg tctcatagtt acttcactta cagagggttg gacaaaattt gtatctaacc
     5641 gcgaacatct tttttcacca ttggataaca tatctattta taccgccaac gatacagttg
     5701 agttgttaga tgtaatgtta aaagcctacg agcaaaaatc cagcaacccg ccggttgatg
     5761 ctaagtcgga caatgctaca ctttcggctt actttgaaaa agtattaccg gaatacgatc
     5821 gtgaaaaagt gcatatcagc gatattaaaa agtttattaa atggttccag attttggatg
     5881 aaaagggcat tttagcaact gaaattgaag aaaaagcaaa agaagaagct gcagctaaaa
     5941 ctgaagaaac tactacaaag gaataatcaa cattataaaa aagcaaaacg ccaatccggt
     6001 ttcaacctga ttggcgtttt ttgttaaatc aatttaaact ataattatta attcttaaaa
     6061 aattgtttgg taagtgagcc ttgtgcattg ctgatacgaa caaaatacat gcctttgttc
     6121 aaattatcaa ccggcataac aaatgtgcca ttggcatatt gatgtttgtt ggaaagcacc
     6181 aaccgtccgt gtatgtcgat tatatcgata acaattggat tattaaccgg tgtgctgaca
     6241 gtaacagcaa gttcatcctg aacaatgcca tgaatatcaa aaaggatatc attcatctct
     6301 tcgatactaa gcggatgatt atcagggtct tctgtaatag ttactgaaga ggtataaata
     6361 tgatctccgg aagcatcatc ttcatcatca cttgcattta cggcaacata aaaagtaaca
     6421 tcaccacttg cttcctcagg ggtccagtta aaagtccagg tttttgaatc tacgccatcg
     6481 gttccggcat tggtatgcgt aatatattta cctccacctg taaattttgt ttggtcggaa
     6541 tcaatgagtg ccatttcacc catttcatca ccttcaagat tttgtggtga agcctggaag
     6601 ccaaatcgtg taatgcctat ttcttcgata gtaaccgtaa tgaggtaagt ttccccaacc
     6661 agataacctg atgccggaac atcggtagca attaaaccat ctttttcaga agcagaacct
     6721 gtatgacaat caacatgcgc gcaggtttgg tcatctcccg gcgagcctgt attccctgat
     6781 ggtgcgccgt cttcttttcc ataactgaat ttagaaagtc cggtgataat aagaataagg
     6841 agtgcgcaga ggaatacatg tgtcgattga aatcttttca taatgtaatt tgatacaata
     6901 aaggtgcctg aaaaaaatgt atgtttatac atacaattac aaaaatgaca atttacttac
     6961 ataattccaa acgggaagtc aggtttcaag aatttgcgac gcggcttttc ctgcccaaac
     7021 tttgtgtata attttttcga tgaccccttt ttcatttatt aaaaaggtga tacgatgaat
     7081 tgtggtaata gttcgcccca taaataactt ttcaccataa acaccatatt ttttaacaat
     7141 ggttaagtcg gtgtcggcaa gcagggtaaa tggcagttta tatttatctg caaatttttt
     7201 ctttgactcg acaggtgcat gagacacacc gattattttt atgccggctt tttttaatgc
     7261 tgcataatta tcccgcaaat tacaagcttg tttggtgcaa gtttcggtat cgtcgttggg
     7321 gtaaaagtat aaggcaaacc gctgcccttt taatccggtg gaagaaactt tattgccatc
     7381 ctggtctagt gcattaaatg caggcgcttt atctccgggt ttgagatgtg taatggaaag
     7441 taattcagcc atacgttaat taaaaattcg ggcgaagatg tgaagtggca taaaatgcag
     7501 caatatgttt tactacctct tcaggatcat cggtcatata aaataaatct aaatcttctt
     7561 cgctgatatt atgttctttg tgcaacattg tttcacgcac ccaggccatc aaacctttcc
     7621 agtaggattt acctacaaga ataaccggaa ctcgttcaat tttatgtgtc tgaattaaag
     7681 taagtgcttc aaacatttca tctaaagtgc caaatccccc gggcataaaa ataaatgcct
     7741 gtgcatattt cacgagcatt acttttcgaa caaaaaagta tttaaaatta atgagtttgt
     7801 ctctgtcgat atatggattg tgactttgtt cataaggtaa ttcaatatta agacctaccg
     7861 atttaccgcc ttctttatag gcacctttat tacctgcttc cataataccc ggtccgccac
     7921 ctgttattat tccaaatcct tcatgtacca gtttagctgc aatctgctca gataatttat
     7981 aatatttgtg gtcaggtttt gttcttgcgg aaccatatat tgaaacacat gggcctattt
     8041 tatccatggt ttcaaaacca tcaacaaact ctgccattat tttaaacatt ctccagcttg
     8101 actgtgcact tagctcggtc cagtctttta ccttaaacaa tttcttctta cgatatttct
     8161 cagccatata attatgattg tactaattta ttagagttcg aggatgaaat taacaataat
     8221 ccgataaaag tcaaaattcc gttaatcagt agtaattcat atcctatttg gtatccattg
     8281 aataatgctt ttgaatgcat gttcaaataa taacaaataa aaggtggtaa taaacaaact
     8341 aataatacca ctttatccat aatatttctt tttgtgataa ttccgaaagc aaacaaacct
     8401 agcagcggac cataagtata tcccgccacc gcaaacactt gattaattac tgcatcatta
     8461 ttaattgcgt taaaaacaat tatcacaatt aatagtatta cagcaaacag cacatgaata
     8521 atatgcctgt tttgtttttg tttagtcgat tcggtttgcg taacatccca tcctaatata
     8581 tcgatacaaa aagacgtagt aagtgtggta agcacagagt cggcacttga aaatgtggca
     8641 gcagcaattc caagaataaa aacaacagcc gcaaaaatac cgagatagtc gagtgctaaa
     8701 ataggaaaca aatgatcggt gcgagccggc gaatcgatac ccataacgcc gatatattcg
     8761 tataacaaag cacctaatga cacaaacaac aaattaataa aaataagcac tacgctaaac
     8821 cataaaatat ttttctgcgc ttccggtaat gtgcgcatac tgagattttt ttgcatcata
     8881 ttctggtcca aacccgtcat tacaattgca attaatgcgc cgctgataaa ttgtttccag
     8941 aagtaattcg gagcttttac atcccagaaa aatatttgat tgtgttcact atttctcacg
     9001 gtatcaataa taccgttaaa atctaaattc aacgcaccgg cgattgcaat tattgaaaga
     9061 attacaccca ttaataaaaa tgaggattgc aaagtatctg tccacaccag tgttttaatc
     9121 ccacctctat aggtgtataa taatataaga attattacaa tcgaaacggt tacttcaaat
     9181 ggtacgcccc atggttggaa aataaataat tgcagtaccg ttgccgatac atataatcga
     9241 aaagctgccc ctaccaatcg tgaaacaata aaataaaacg aacctgtttt ttgtgcgttt
     9301 ttaccaaatc gctgccccag ataagtatag atagaagtta aatttaatcg ataatataac
     9361 ggcagtaaaa cagtagctat tacaaaatat ccaagcagat aaccaattac aatttgcata
     9421 taaccgaact cctgagtagc aacattaccc ggtacagaaa caaaggttac tccgctgagt
     9481 gaatcaccaa ttaatccaaa tgc
//
LOCUS       DAORKW010000077         9460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_176_length_9460_cov_5.771611, whole genome shotgun sequence.
ACCESSION   DAORKW010000077 DAORKW010000000
VERSION     DAORKW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9460
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176_length_9460_cov_5.771611"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1127)
                     /locus_tag="PLB46_04780"
     CDS             complement(93..1127)
                     /locus_tag="PLB46_04780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF75864.1"
                     /translation="MNPKVCIVLVNYKTWQDTLECMESILRSDYRNYQIVVVENGGDD
                     DSWEQMLKWSRGENVVAIETTNPLHHLTFPPVPKPVLYFRADVGESDTMRLGPLDGIM
                     PILFVKSKINLGFAGGNNVGIRYALNSNFDYVWLLNNDTVIEPNAITELVHYCENKAA
                     KKEKVGIVGSKLMVYHQPEKMQGVGAIFNKYSGKSKIIGAQEIDRNQYDNTEISCNYV
                     IGASMFVSQPFLLDVGLMSEEYFLYNEENDWSARAKLKGWKVGVALKSKVYHKQGAST
                     GNSPKKSKWQIKALNYKYRGKIKLYKKYYRAQLPFLYLHLVTRSFKYIAKGNMSEAKV
                     IYGAIFNTKF"
     gene            complement(1134..2093)
                     /locus_tag="PLB46_04785"
     CDS             complement(1134..2093)
                     /locus_tag="PLB46_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HRF75865.1"
                     /translation="MQSNTSILPNFIVVGANKGGTTSIYHYLRQHPEVYLSPVKEPHY
                     FSKDIDVDLFKREFAQNKLQDIEKYVNGDMSEEYHAAFIRDETQYRKLFKNVKGEKAI
                     GELSTSYLYSTVAVNEIKKLVPNCKIIICLRNPIDRAYSHYRMNLWTGNSNEFDFHKA
                     LLDDFNHDPKVWGNAHLYTEIGMYYQQVKRYLDAFGAENVKIIFTEDMKKNASGVIKE
                     LYNFIGVDSSFVADTSKQYNEVYTPKYKNLTWFLNKSGIRPLMKKFSPRFIKNIIVKA
                     LYKGKSDKGEIPAAAKQFLKEKLQADVMQLGVLLNKDLSNWLK"
     gene            complement(2093..3091)
                     /locus_tag="PLB46_04790"
     CDS             complement(2093..3091)
                     /locus_tag="PLB46_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75866.1"
                     /translation="MQLISADKRILVIGSLNANPADRAISRDNIRSMQVIDENQQILP
                     LNYFNTWGELGMSSLCWHETDPYTIYLASSNELFKFYLKENRYEKLDIPNLTDIHEIT
                     MIGNKVWISNTKHDEIVSYDIVANKIGERINLTDFKSNSPEEGEGDEDVEVVDKFHCN
                     LVFEGYEGDLYILVHHTSGRQLMKRIAQKFIKSQGNGGVVNIIKRKRHPLNFKAPHSV
                     RKINNQYWVFDSGHFELKIFDKNWNYLRTMDTKGFGRGGDLDTNRNIYYAGVSETRKR
                     YLGLFPGGEAVPNMVQVFAVGEYKLLGSIKIPNIEQINNVYCMQKDVVDLLLNFKG"
     gene            complement(3108..4445)
                     /locus_tag="PLB46_04795"
     CDS             complement(3108..4445)
                     /locus_tag="PLB46_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75867.1"
                     /translation="MGILAQEKGIFKEPMSVFGKTLMLVVFILFTTRLLTRTPLVDFY
                     TPFEILWYTFIITFTFIYAFYLFLVRKTVDEIHFYYVLIILMPIWAGFFAVLSEGQPF
                     MQGLSSQRHWYGISGIFVMIYMLQTRFITIGDLFRAMKWLGWGTMVYYLLCYMFLDPK
                     QYLDYSFVGYSDIKGGYRFKFGIDFIAFLSVYYTVKYVKQRDSSSLFYSGLNLSYLFF
                     LHQGRSVMLSVVATLAVFYMFEVNWQRALRIFVTIGIVFMVLMGVLFAIIPDRVNDYV
                     VQYTSLATVVFTGNASGESSVDARLEEIGILLIYLQDYSIGWWVGVGKLGAESQSVGT
                     DHDITTVPPGDIGILGSIMTYGIIGSLIIYMQFLIAFRSGKFIRRFKHEPFMIASKYF
                     LIFSFLSSLAKGYLFMQPGSTAIFIMVIYAYKLLEVQMDKQGLPESLVIKNSF"
     gene            complement(4447..5316)
                     /locus_tag="PLB46_04800"
     CDS             complement(4447..5316)
                     /locus_tag="PLB46_04800"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRF75868.1"
                     /translation="MQQNFVCIGAQKAGTTTLADILSQHSDICIPPIKETKYFLFDAD
                     YQKGKSFYDSYFTNYKNQKAVGEFDPDYLLFPFTAQRIFETLGPDVKIIVVLRNPADR
                     AYSHYLMTKRKGLEPFDFLEAIKEEANRKSDIKTQKIFAYKERGMYGQQLKQFLNVFK
                     PENFLFLVFEEDFLKNREITITKVQQFLQVKEQNLNLDVHSNEAGEAKSESLNELVRK
                     PNFVKRILKAILPSKSFRKKVRKMFIRQNMQKVSAPKLENEQRKQLIADYYFDDIKLT
                     EQLINRDLSVWYK"
     gene            complement(5321..6223)
                     /locus_tag="PLB46_04805"
     CDS             complement(5321..6223)
                     /locus_tag="PLB46_04805"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRF75869.1"
                     /translation="MNRWPNFLIVGTARAGTTSLHEFLGGHPDIYMPLQKEPCFFTFY
                     NEQPSFKDSKHRYTTTVESYSELFDGHQEKIMGESSTPYLYFHQKSIEHIKQLVPDYR
                     KLKILIVLRDPAERAYSQYMHNRRDLLETATFETAIAEEKKRMAENWHFDFFYVDKGY
                     YYEQVKNYLDNFDNVKIVFYDTLEKNPDKLLSEIYEFLEVPQQGQKELVKRNQSGEMK
                     VKWIKQIITTRKNPILNFFRKLMSRETKKKLRNWVKDKLLRYNLKKTEMNPDTRKQLI
                     ELYKNDIIKLQSLVNKDLSDWMRI"
     gene            complement(6225..7733)
                     /locus_tag="PLB46_04810"
     CDS             complement(6225..7733)
                     /locus_tag="PLB46_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HRF75870.1"
                     /translation="MISKLKKKFNKKFKDEDFSEILKKGSGAFGISIVGRFIGYLLLM
                     VIGWIYGQEAMGVYTVGLSILSIVGIFGRFGVDMALSRFVAQYGSIGRWDLVQHTYRK
                     ALIIIIPLGIFLSAATYFILPYYTDNLFDPTDANFKNYTFYMQLFAASIIFYVLGGLS
                     EECIKGLKKTAQYNWIAQVINQLVAILFLLIFGLFSKDQLWVNLSYCLGIFVAFLIAQ
                     IYWLHYLKNPEIAGSEFKGKKNNKQKNKPVQLEAANKIIAPEEPITTTELLKISAPMM
                     SAKYLTMIYTWLDILILAWYAAETKVAIYRVATRLTSLATIPLISINSIAGPKFAEAH
                     GKNDQKQLKNSVKQSTRLIFWSCIPILAIFLIFPKFSMWTFGKDFTGTEAVLVFVIVT
                     LGQAANVLTGPVTVLLNMTGRQKITMYYAFATVIIDVSLNLILIPLYGIIGAAIATSV
                     ARTVLNFGLAFQIYYTMGIVTIYNPFSDIRDMLNRGKKNKNRVDLNQSDEHE"
     gene            complement(7730..8860)
                     /locus_tag="PLB46_04815"
     CDS             complement(7730..8860)
                     /locus_tag="PLB46_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75871.1"
                     /translation="MKKLFIRIGILLLIVYTICQLAISSMPFSWGNTRLNTKFQSYKE
                     NHEQYNTLLIGASTTYRHINSTTFDSIVNAQYPELHIKSYNFGIPANRTPQSLQMLDA
                     LLDYDMRNIKHVVIDMSELTKMGADNLHKKEMLYWYNWGNIGDVMKASYESEKGVNKF
                     GVPMLHAFSFGEKSLLFGNGPTVIMQHAGLNIEPLSVGPDNNGFYSLDQEMHDDPEGD
                     LAIRYNELRTPDTIAYRTKRCQELVDHYKTASKNPNKTIEENLKDVIEYCEKNGISVT
                     IMLSQRLGERYEYLIPLYNQLPEKNRIGFQDPSAYPALNDRDNLFDLAHLNENGSKVF
                     THIFAGEWLKRLEMQNIIQPKPAPVVDQDTLNLNTTADGSGI"
     gene            complement(8864..>9460)
                     /locus_tag="PLB46_04820"
     CDS             complement(8864..>9460)
                     /locus_tag="PLB46_04820"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRF75872.1"
                     /translation="DYLYIPLGGSRVSFGRMLFNNWFTLTIMGLWHGANWTYVMFGFI
                     HGFFIVMSRIWDRYFPKLTLPQLLPGMKAFTGVFLAFWIFNLTCIPDIFFRSNTVGDA
                     WGVIQNIFSPDTTNYLIHNSAAKGAVPSVIEFWVSIACVALLLFTDYKLYTNKSVGID
                     KFVVAKPFYARWSFYVVFLVIVTLFAYTSKSVFIYFAF"
BASE COUNT         3011 a         1869 c         1442 g         3138 t
ORIGIN      
        1 ataattagct tttaattcct ttgcaattgc actacttgca gtttggggta attcaacaaa
       61 tacatatttg tgcttgtgac tgataatcat aattaaaatt ttgtgttaaa aatggcacca
      121 taaataactt tcgcttcact catgtttccc ttggctatat atttaaaact tcgggtaact
      181 aaatgaaggt ataaaaatgg caattgcgcc ctgtaatatt ttttatataa tttaattttt
      241 ccgcgatact tataattaag tgctttaatc tgccatttac tttttttcgg actgttgcct
      301 gttgaagcac cttgtttgtg atatacttta ctttttaaag caacacccac tttccatccc
      361 ttcagcttag cccttgcact ccaatcgttt tcttcattgt acagaaagta ctcttcactc
      421 atgagcccta catctaataa aaatggctgc gacacgaaca tagatgcgcc tatcacatag
      481 ttacaggaaa tttctgtatt gtcatactgg ttgcggtcaa tttcctgtgc tccaattatt
      541 ttagactttc cggaatattt attaaaaatt gcaccaacgc cctgcatctt ttccggctga
      601 tgataaacca tgagtttact gccaacaata cccacctttt cttttttggc agctttattt
      661 tcacaataat gcactaattc agtaatggca ttgggttcaa ttaccgtatc gttatttaat
      721 aaccacacat aatcgaaatt gctgtttaat gcataacgaa tacctacatt attgcctcct
      781 gcaaaaccga gattaatttt acttttaaca aataaaatag gcataatacc gtccaatggt
      841 cctaatcgca tagtatccga ttcacccaca tctgctctaa aatataaaac aggttttgga
      901 acaggcggaa atgtcaagtg atgcagcgga tttgtggttt ctattgcaac aacattttcg
      961 ccacgtgacc atttaagcat ttgctcccaa ctatcatcat ctcctccatt ttctacaaca
     1021 acaatctggt aattgcgata atcgctgcgc aatatacttt ccatacattc cagcgtatcc
     1081 tgccatgttt tataatttac taaaacgata caaactttag gattcatggc aatttatttt
     1141 aaccaattgg ataaatcttt atttaacaat acacctaact gcattacatc tgcttgcaat
     1201 ttttctttaa gaaactgttt cgctgcagca ggtatttcac ccttgtctga tttaccttta
     1261 tataaagctt taacaattat gtttttgata aatcttggtg aaaacttttt catcaacggg
     1321 cgaattcccg acttatttaa aaaccatgta aggtttttgt attttggtgt atatacctcg
     1381 ttgtattgtt tagaagtatc agcaacaaaa ctgctgtcca caccaataaa attgtataat
     1441 tctttgataa ctcctgaagc attttttttc atgtcttcag tgaaaataat ttttacattt
     1501 tctgccccaa atgcatccag gtagcgtttt acctgttggt aatacatgcc tatttccgta
     1561 tacaaatgcg cattacccca caccttcggg tcgtgattaa aatcgtcgag taaagcttta
     1621 tgaaaatcaa attcgttgct attacctgtc cataagttca tgcggtaatg tgaataggcc
     1681 ctatctatgg ggttgcgcaa acaaataatg attttacaat tcgggactaa cttttttatt
     1741 tcgtttacag caactgtaga atataggtaa gaagtactta actcaccaat tgccttttcg
     1801 ccttttacat ttttaaataa cttcctgtat tgggtttcat cgcgaataaa agctgcatgg
     1861 tattcttcgc tcatatcccc attcacatat ttttcaatat cctgtaattt attttgtgca
     1921 aattctcgtt tgaataaatc tacatcaata tcttttgaaa aataatgcgg ttcctttacc
     1981 ggggacaaat acacttccgg gtgctggcgc aagtaatggt aaatagaagt ggttccacct
     2041 ttatttgcac cgactactat aaaattgggt aatatggaag tattagattg cattaacctt
     2101 taaagtttaa caataaatct actacatctt tttgcatgca atatacattg ttaatttgtt
     2161 caatgtttgg tattttgata gaccctaata atttgtattc gccaacagcg aatacctgaa
     2221 ccatgtttgg cactgcctca ccgcccggga ataaaccgag ataacgtttt cttgtttcgc
     2281 tcacacctgc ataatagata ttacggttgg tatctaaatc accaccacgt ccaaaacctt
     2341 tggtatccat ggtgcgcagg taattccagt ttttgtcaaa tatttttaat tcgaaatggc
     2401 cgctgtcaaa aacccaatac tgattattaa tttttcttac actgtgtgga gctttgaaat
     2461 tgagtggatg acgttttctt ttgatgatat ttactactcc accattgcct tgacttttaa
     2521 taaatttctg agcaattctt ttcattaact ggcgacctga ggtatggtgc actaaaatat
     2581 ataaatcgcc ttcataccct tcaaatacca ggttgcaatg aaatttatct acaacttcta
     2641 cgtcctcatc accttcgcct tcttcaggtg aatttgattt aaagtcggtg aggttaatac
     2701 gttcgccgat tttattggca acgatatcat aacttacaat ttcatcatgt tttgtatttg
     2761 aaatccatac tttattgcca atcatggtaa tttcatgtat gtctgtcaaa ttggggatat
     2821 ccaatttttc gtatctgttt tccttcaaat aaaacttgaa caattcgtta ctggaagcca
     2881 ggtaaatcgt ataagggtcg gtttcatgcc agcataaact gctcatgccc aattcacccc
     2941 aagtgttaaa ataattgagt ggtaatattt gctgattctc atcaattacc tgcatactcc
     3001 taatattatc tctgcttatt gctctatcgg caggattggc gtttaaagac ccaattacca
     3061 gtattcgttt atctgcacta attaattgca tggtataatt aattttttta aaaagaattt
     3121 ttaataacta aactttcagg taacccctgt ttatccattt gcacctctaa taatttatag
     3181 gcgtaaataa ccataataaa tatcgccgtt gacccgggtt gcataaacaa atatcccttt
     3241 gccaacgaag ataaaaaact aaatattaaa aagtatttgg aagctatcat aaacggttca
     3301 tgcttaaatc gcctaataaa cttcccactt ctaaatgcta tcagaaattg catgtaaata
     3361 attagtgacc caataatacc ataggtcata attgaaccta atatgccgat atctcctggt
     3421 ggcactgttg taatatcgtg atcagttcca actgattgtg actctgcacc taactttcct
     3481 actcctaccc accaacctat tgaataatcc tgcaagtaga ttaacagaat acctatttcc
     3541 tctaaccttg catcaacgga actttcaccg gaagcattac cggtaaacac cactgtcgcc
     3601 aaactggtat attgaactac gtaatcattt acacggtcgg gaataatggc aaataataca
     3661 cccattaaaa ccataaatac aataccaatc gtgacaaaaa ttcgcaatgc cctttgccag
     3721 ttaacttcaa acatataaaa cactgcaagg gtagccacaa ccgacaacat taccgaacgt
     3781 ccctgatgta aaaagaacag gtatgataag ttgagtcctg aataaaacaa acttgaacta
     3841 tcgcgttgtt tcacatattt aacagtataa tacacagata aaaatgcaat aaagtcgata
     3901 ccaaatttaa aacggtaacc ccctttaata tcgctatacc ctacaaacga ataatctaaa
     3961 tattgtttag ggtccaaaaa catgtaacat aataaataat acaccatggt tccccaaccc
     4021 agccatttca ttgctctaaa caaatcgcca atggtaataa aacgtgtttg gagcatataa
     4081 atcatgacaa aaattccgga aataccatac cagtgtcgtt gtgaggataa gccctgcatg
     4141 aatggctgac cttcacttaa cactgcaaaa aatccggccc aaatgggcat gagaataatt
     4201 aggacataat aaaagtggat ttcgtcaacg gttttacgca ccaaaaacaa ataaaatgca
     4261 taaataaaag taaatgtaat gatgaatgta taccacagaa tttcaaaagg tgtataaaaa
     4321 tcgaccaatg gtgttctggt aagtaatcgc gtagtaaaaa ggataaatac caccagcatc
     4381 agtgttttac caaacaccga cattggttct ttaaatattc ctttttcctg tgctaaaatt
     4441 cccatattac ttataccaaa ctgataaatc gcggttaatc aattgttcag ttaatttaat
     4501 atcatcaaaa taataatccg caataagttg cttacgttgt tcattttcca attttggcgc
     4561 acttactttt tgcatatttt gccggataaa catcttacgc accttttttc taaacgattt
     4621 agaaggtaaa atggctttta aaattcgttt aacaaagtta ggctttctta ctaactcatt
     4681 caatgattcg ctttttgctt cgccggcttc attgctatgc acatcgaggt tcagattttg
     4741 ctcctttacc tgtaaaaact gctgcacctt tgttattgta atttcgcgat tttttaagaa
     4801 atcttcttca aaaaccaaaa aaaggaaatt ttcaggttta aacacattta aaaattgctt
     4861 caactgttgg ccatacatac cacgttcctt gtaagcgaaa atcttctgtg ttttaatatc
     4921 cgatttccta ttcgcctctt cttttattgc ttccaaaaaa tcaaatggtt ctaaaccttt
     4981 gcgtttagtc attaaataat gcgaatacgc cctatcggca ggattgcgca atactacaat
     5041 aatctttaca tcaggtccca atgtttcaaa tatccgctga gccgtgaacg gaaataacaa
     5101 ataatcaggg tcaaattcac caacagcttt ttggttttta taattggtaa aatagctatc
     5161 gtaaaatgat ttaccttttt gataatcagc atcaaataaa aagtacttcg tttcttttat
     5221 tggcggaata cagatatcac tatgctgact gagaatatca gccaacgtag tagttcctgc
     5281 tttttgagca ccaatacaaa caaagttctg ttgcatatat ttatattctc atccaatcgc
     5341 tgagatcttt gttcaccagt gattgcaatt taataatatc gtttttatat aattcaatta
     5401 attgtttcct tgtatccgga ttcatctcag tttttttgag attatatctc agcaatttat
     5461 ccttcaccca attgcgcaat ttcttttttg tttcgcggct catgagtttt ctgaaaaaat
     5521 ttaatatagg gtttttacgc gtggtaatta tctgcttaat ccatttcact ttcatttcac
     5581 cactttgatt acgctttacc aattcttttt gcccctgttg aggcacttct aaaaactcgt
     5641 aaatttcgga caacaattta tccggatttt tctcaagcgt atcgtaaaat acaattttta
     5701 cattgtcgaa attatccaga taatttttca cctgctcgta ataataacct ttatcaacat
     5761 aaaaaaagtc gaaatgccaa ttttccgcca tacgtttttt ttcctcagca atagccgttt
     5821 caaaagtggc agtttctaat aaatcacggc ggttgtgcat atattgcgaa taggctctct
     5881 cagcagggtc gcgtaataca atgagtattt ttaattttct ataatcgggc accaattgtt
     5941 taatatgctc aattgatttt tgatggaaat acaaatatgg cgtagatgat tctcccatta
     6001 ttttttcctg atggccatca aataattccg aataactttc tactgttgta gtatatctgt
     6061 gtttagaatc tttaaaagaa ggttgttcgt tgtaaaaagt gaaaaaacat ggttcttttt
     6121 gcaatggcat ataaatatca ggatgcccgc cgagaaactc gtgcaacgat gttgttcctg
     6181 cgcgtgcagt acctactatc aaaaaattag gccacctgtt cattttattc gtgttcgtcg
     6241 ctttgattta agtccaccct gtttttattt ttcttacctc gatttagcat atctctgata
     6301 tcagaaaatg ggttatagat ggtcacaata cccatggtat aataaatctg aaaagccaat
     6361 ccaaaattca ataccgttct tgcaactgat gttgctattg ctgctccaat aattccatac
     6421 aaaggaatta aaattaaatt taaactcaca tcaataatta cagtggcaaa ggcataatac
     6481 atggtaattt tttgcctgcc ggtcatattt aataaaacag taaccggacc ggttaaaacg
     6541 tttgccgcct gacctaaagt tacaataaca aacaccaaaa cagcttctgt tccggtaaaa
     6601 tctttaccga aagtccacat gctgaatttc ggaaaaatta aaaatatagc caaaataggg
     6661 atgcaagacc aaaaaattaa tcgagtagat tgtttaacgc tgtttttcaa ttgcttttgg
     6721 tcatttttac catgagcttc agcaaactta ggccctgcaa ttgagttaat agaaattaaa
     6781 ggtattgtgg ccaaagaagt taatcgtgta gctactctat atatagcgac tttggtttca
     6841 gcagcatacc aagctaaaat cagaatatct aaccaggtat atatcatagt cagatatttt
     6901 gccgacatca taggtgccga aattttaagc aattcagttg ttgttatagg ttcctccggg
     6961 gcaattatct tatttgcggc ttctagttgt accggtttat ttttttgctt gttgtttttt
     7021 ttccctttaa attctgaacc tgctatttct ggatttttta aataatgcaa ccaataaatt
     7081 tgtgcaatta aaaaggcaac aaatataccc aaacaatagg ataaatttac ccaaagctgg
     7141 tctttggaaa acaatccgaa tatcaacaaa aatagaattg ccaccaactg atttatcacc
     7201 tgtgcaatcc aattgtactg tgcagttttt ttcagacctt ttatacactc ttcacttaaa
     7261 ccacctaaaa cataaaaaat aatagatgct gcaaatagtt gcatgtagaa tgtataattt
     7321 ttaaaattag catcagttgg gtcaaataaa ttatccgtat aataaggtaa aataaaatag
     7381 gtagcagcag ataaaaatat accgagtggt ataataatta ttagtgcttt tcggtaagta
     7441 tgttgaacta aatcccagcg accaattgaa ccatactgtg caacaaatcg ggataatgcc
     7501 atgtcaacac caaagcgacc aaaaatgccc acaatactta aaatacttaa acctacggtg
     7561 taaacaccca tagcttcctg accgtaaatc cagcctatta ccatgagtaa caggtaacca
     7621 ataaatctac caacgatact aataccgaaa gcaccggagc cctttttcag gatttcagaa
     7681 aaatcttcat ccttaaactt tttattgaat ttctttttta atttggagat catatgccac
     7741 ttccatccgc agttgtattc aggtttaaag tatcttggtc aactacagga gccggctttg
     7801 gctggataat gttttgcatt tccagacgtt ttaaccactc gcccgcaaaa atatgtgtga
     7861 aaactttaga accgttttca tttaagtgag ccaaatcaaa tagattatca cggtcattta
     7921 atgccggata tgcacttggg tcctgaaaac caattctgtt tttttctggt aattgattgt
     7981 ataagggaat taaatattca taacgttccc ctaatcgctg agataacatg atagtaactg
     8041 aaataccgtt tttttcgcaa tactcaatta catcttttaa attttcttct attgttttat
     8101 tcgggttttt tgatgctgtt ttataatggt caactaattc ctgacaacgt tttgttctat
     8161 acgcaattgt atcaggagtg cgtaattcat tataacgtat agctaaatcg ccttccgggt
     8221 catcgtgcat ttcctgatct aacgaataaa atccattatt atcaggtcct accgatagtg
     8281 gttcaatatt tagaccggca tgttgcataa ttactgttgg gccattgcca aataacaacg
     8341 atttttcacc aaatgaaaaa gcatgcaaca tgggcacacc aaatttgttt accccttttt
     8401 ccgattcgta agaggccttc attacatcac ctatattacc ccagttgtac caatacaaca
     8461 tttctttttt gtgtaaattg tcggcaccca ttttagtcaa ctctgacata tcgattacaa
     8521 catgtttaat attacgcata tcataatcga gcaatgcgtc taacatttgc agcgattgcg
     8581 gtgttctgtt tgcaggtata ccaaaattgt aagatttaat atgcaactca gggtactgtg
     8641 cattaacaat agaatcaaac gtggttgaat tgatatgccg ataagtagta ctcgctccaa
     8701 ttaacaatgt gttgtattgc tcgtgatttt ctttgtacga ttgaaatttc gtgtttaacc
     8761 tggtattacc ccatgaaaac ggcatgctag atatagctag ctgacagatt gtatatacaa
     8821 tcagtaataa aatgccgatt cttataaaga gttttttcat agtttaaaac gcaaaataaa
     8881 tgaacaccga tttagaggta tatgcaaata aggtaactat cactaaaaat accacataaa
     8941 agctccaacg ggcataaaat ggtttagcta ctacaaattt atcgatacct acactcttat
     9001 tggtgtataa tttataatca gtaaacaata ataaagctac gcatgcaata ctcacccaaa
     9061 actcaataac acttggtact gcacctttgg cagcactatt atgaattaaa taattggtag
     9121 tatccggtga aaatatgttt tggataactc cccaagcatc acctacagta tttgaacgga
     9181 agaaaatatc cggaatacag gttaagttaa aaatccaaaa cgccagaaat acacctgtga
     9241 atgctttcat tcctggcagt aattgcggca atgtgagttt aggaaagtac cggtcccaaa
     9301 tccggctcat gactataaaa aagccatgaa taaacccaaa cataacatag gtccaattag
     9361 ctccgtgcca taaacccata atagttaagg tgaaccagtt attaaataac attctgccaa
     9421 acgaaaccct gctacctccc aaagggatgt agaggtaatc
//
LOCUS       DAORKW010000078         9429 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_177_length_9429_cov_5.533497, whole genome shotgun sequence.
ACCESSION   DAORKW010000078 DAORKW010000000
VERSION     DAORKW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9429
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177_length_9429_cov_5.533497"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1921
                     /locus_tag="PLB46_04825"
     CDS             <1..1921
                     /locus_tag="PLB46_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF75873.1"
                     /translation="STCPYLSSAQGADLLWQKSFGGAQTDIGYSACNAVDSGYIIATT
                     TYSSDGDVSENAGLRDFWIINSDLSGNIIWQKNYGGSSLEELGAVLKTSDSCYVFGGT
                     SYSSDGDIGLNFGGSDCVVMKLSKEGEILWLHVFGGSKNDWLTSIIQTIDGGYIFTGH
                     SRSDNGIFADHSGSTDYPDLIVVKLNSLGELEWSKSYGGINSDEGNAVIQTTELDYVV
                     VGYTSIWEPTFDFDYWILRIDVDGNIIWDKKYGGTKQDYAFAVAELYSKNLIVTGEAW
                     SDDGDISDSHGLQEAWTILLDEVDGEIIWKKSFGGSCAETGRDILMHADSTISIIGST
                     CSPDDGDILGHHGDFGYSDYWIFNIDTIGIIRWQQCFGGTETDQAYNASLTLDTSILI
                     TGSARSNDFDITDHHSGDDAWTIKIKNACNAMKYYPDLDDDGFGDIASMYIYSCNDSI
                     GFESNNLDCNDSIFSINPSVKESCNYFDDNCNGLIDEGFAYLHTFEDFDGDVFGNENI
                     DSISCVIPEGFVLDSTDCDDNNAAIYPGAVELLNGLDDDCDQIADEGLAIDNQEQFDF
                     SIYPNPTDNFVTIINDLNLTATVQVFNNVGQAVIANKEFHGNEILLFENLPSGIYLIE
                     ITTSNKKESIVLIRN"
     gene            1947..2741
                     /locus_tag="PLB46_04830"
     CDS             1947..2741
                     /locus_tag="PLB46_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75874.1"
                     /translation="MLNRNKPELIFLAVFCVIAGIYFIIYYHFHVDENQFGAMGDSFG
                     GFVGPVAAYFAAYFAYRTYQVQKADLKATTDGLKEERDNKKIQEVKIAIQEKIDTIAD
                     SFEFLKENVDRLYYKSVNKEVFEGVAAIEQIFIDLNKQSGPTHLMAKQTVKSLEVCNE
                     IFNYNTENTMAFNDNKEMQVLLIWELILIYDSMFYTTYENYNMKHLFETSITTIKKLR
                     NSGDYSNTQWLNEQGLTDLYDSVNLARANYKNLSFAFNQLRRRQIM"
     gene            complement(3100..5466)
                     /locus_tag="PLB46_04835"
     CDS             complement(3100..5466)
                     /locus_tag="PLB46_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF75875.1"
                     /translation="MKDLVTPFALCLIFQSCIFAQVPEIHWQKALGGSGYDASYSICE
                     DIGGGFIVTGDTWSNDGDVTEYAGSGDIWVAKLDSIGEIEWEESYGGSGWEQAFQIKQ
                     TQDLGFIIVGFSTSTDGDLSENNGADDVWIIKLDSIGNIQWQKSYGGTSSDRGNSVTI
                     IDDGGYLITGLSSSNNGDVSGNHGEADYWILKINSDGDLVWQKCYGGTEDDQAQSAIK
                     TLDGGFLIVGHSSSNNGDVFGNHGLGDFWVIKITEDGVLQWQKAYGGTNYEDAFSVSN
                     TADSGYIILGYTQSNNGDISGFHGGVSDYWVIKIDSLGVLMWQKCLGGSSSEEGFSIY
                     RAIDTSYILAGHSGSNDGNVTGHHGLAGYSDYWIVNIDSNGNIIWEKSLGGSNTDEAL
                     SITQASDEGYIVTGWARSTDGDVTDHNGIGNNWDFWVVKLAASCTPTLFYADSDEDGY
                     GDLLNDSLSCNLPIGYVGDSSDCNDDDPFTYPSAEDICNTNDDNCNGLIDEDAIFVTY
                     FADVDGDDFGDTFNDSIACFELEGYVINSEDCNDLNVEISPAASEICNDIDDNCDFDI
                     DEGLTTYTLYKDEDGDSFGNSDIFINSCLAVFIGYVSDSTDCDDANNLIYPGAIEICD
                     YLDNDCDGFVDDNLTYIHSFEDADGDDFGNINVDSLSCSIPDGFVEDDADCDDTNPNI
                     YPGADEILNGLDDDCNLLIDEGLSVSESILNTLKIYPNPTDNILYVEYTGFDGSIIEV
                     INIAGQILWTQTIVSSLIQIDVRTFTSGIYLLKVKTTDGEASLKFIKE"
     gene            5611..8490
                     /locus_tag="PLB46_04840"
     CDS             5611..8490
                     /locus_tag="PLB46_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75876.1"
                     /translation="MRTKSKHLFNLEKLAKVFLFNLSLFIFLFQGNLLKAENYFYTNY
                     LFEPNIINNCLPDSTFDDSLNVYSDGDTVSLFTSGADTLIVLYASESADTNSSAKLIP
                     FATEDMVISINSNNQPVQHGLYGINVSDLFEHVGEFTPDGTPNPYYLQSWDDIARLDP
                     AVIRAFSGSGSKFMHPLGSDNTDPLNPFVISGLTRNGGYGYCIEEIINYYENTDGTPF
                     DAPPLNLIIADMGLPTPVLSWMNPNEKGAFKEYYNKWNNQPHFDPTVIPDIEDQPLYI
                     NQLIRLVAQIETFNTLSGKVDVILAINILSQTVNEIKDLLNYLVVDNPIWPVQVVGIE
                     LGNESYFPSHNRLIGFSDFDHYWYFINGGNDYQTEFSDPLDFELTDVLSLTMQADHDF
                     IGDLRSLIPFEFKIGIPAMNLPNGAGWAFKTANPEDTLYPPVGNPEGFSPCELSWNQC
                     LALHYDEMVGEDYAFDAVILHPYYTPSNDPAAGFGTNWGRIPTNYDVDPLNLTPNHLD
                     NDILTDNGYQDFVAPMYDYTTPDTRLQTVFEGMVGIPISDDGGLTYYEGTGNIKALIR
                     TRYKESYDEHAGILNFQSADVGPQYKELWTTEWNFHDADHLDFLPIGYLGANQISERL
                     AVYTNTLVHLDLVQEWYLKNLKMNLQPGYRHNFFTYSTIQNIAGGSNITMLSESKQQD
                     QIQKGLIASCAEIDLLPDYYIKKGSYYGFSLLKTIYEKNLYYISSTTSMYIANNNLAP
                     TVFLAPSEKKLFIYYTNIKNIPQRYIIDPNNLVSVYPGALGVSFSAPSILCVDPKQLY
                     STSGRSSILENITNQYPCTTDITDYPDWFDLKSAASIPGFAFVSCPPGVPADCKCVEV
                     PATSVGYVEIPFTLLRLGELEDIYRIYPNPTSEYFIVELVTPFELENNDFQVDILSMT
                     GMLIKSVLTGEGQKIDIQDIPVGVYNVMIRKEGFAVESEVLVKMK"
     gene            complement(8907..>9429)
                     /locus_tag="PLB46_04845"
     CDS             complement(8907..>9429)
                     /locus_tag="PLB46_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75877.1"
                     /translation="QITSIFIKGILFSIYFCININAFGQTLNSEFMEFIREKQQSTNV
                     EMQIPANQNGLETESIIQDLIVPEWNFKELPIQSETAHIPIPIEIKRPASSNIIFEPQ
                     IVKELLKLRLIQNNTVIPVTVNIYDMEGTVFYTANIQNDVEIDISLYPPGTYILYTSS
                     SATTHQFQKVVIQ"
BASE COUNT         3060 a         1640 c         1630 g         3099 t
ORIGIN      
        1 gagcacctgc ccctatttat catcggcaca gggtgctgat ttattatggc aaaagagttt
       61 tggtggggca cagactgaca tagggtatag cgcctgcaat gccgttgatt ctggttacat
      121 aattgcgaca actacatatt catcagacgg agatgtatca gaaaacgccg gcctcagaga
      181 cttttggatt ataaactctg atttatcagg gaatatcatt tggcaaaaaa attatggagg
      241 ttcatcactt gaggagctag gagcggtgct taaaacgtca gatagctgct atgtttttgg
      301 gggtacttct tattcctcag atggcgatat tggtctcaat ttcggtggtt ctgattgtgt
      361 tgttatgaaa cttagcaagg aaggggaaat cttatggctg catgtttttg ggggtagtaa
      421 aaatgattgg cttacatcta taattcaaac cattgacggg ggttacattt ttacaggaca
      481 tagccgttct gataatggaa tttttgcaga ccattccggt tccacagatt atccagattt
      541 aattgtagtg aaattaaaca gtttaggtga attggaatgg tcaaaaagct atggcgggat
      601 taattctgat gaaggaaatg cagtaataca aacaactgaa cttgattatg ttgtggtggg
      661 ctatacaagt atttgggagc caacttttga ttttgattat tggattttga gaattgatgt
      721 agatggtaat attatttggg ataaaaaata tggagggact aaacaagatt atgcttttgc
      781 ggttgcagaa ttatattcta aaaacctgat tgtaacaggc gaagcttggt cagacgatgg
      841 cgatatttca gattcacatg gtttacaaga agcttggaca attttacttg atgaagttga
      901 tggagaaatt atttggaaga aatcttttgg aggctcttgt gctgaaactg gtagagatat
      961 actaatgcat gctgattcta ctatttcaat aattggttcg acatgctcac ctgatgatgg
     1021 cgatatatta ggtcaccatg gtgattttgg ttattcagat tattggattt ttaatattga
     1081 tactatagga ataattaggt ggcaacaatg ttttggtggc accgaaacag accaagcata
     1141 caacgcatca ttaacgctag atacttcaat tttaataaca ggctctgcta gatcaaacga
     1201 ttttgatatt actgaccatc attcgggtga tgatgcatgg acaattaaga ttaagaatgc
     1261 ttgcaacgct atgaaatact atcccgatct agatgatgat ggttttggtg acatcgcaag
     1321 tatgtatata tatagttgca atgattcgat tggattcgaa agtaataatt tagattgtaa
     1381 tgactcaata ttctctataa atccttcggt aaaggaaagt tgtaattatt ttgatgataa
     1441 ttgcaatggg ttaattgatg aaggctttgc atatttgcat acgtttgaag attttgacgg
     1501 tgatgtattt ggtaatgaaa acatagattc aatttcctgt gtaattcctg aaggatttgt
     1561 tttagatagt acggattgtg atgacaacaa cgcagccata tatccaggtg ctgttgagct
     1621 tttaaacgga ttagatgacg actgcgacca gattgcagat gaaggcttag caatcgacaa
     1681 tcaagagcaa tttgattttt caatataccc aaacccaact gacaattttg taacaatcat
     1741 taatgactta aatttaactg caactgttca agtatttaac aatgtcgggc aagcagtaat
     1801 agcaaataaa gaatttcatg ggaatgaaat tttattgttc gaaaaccttc cgtctggaat
     1861 ttatttaatt gaaataacta cgagcaataa aaaagaatcc atagtgttaa ttcgtaatta
     1921 attggaaaca ttatttttaa tagccaatgt tgaatagaaa taaaccggaa ctcatattcc
     1981 ttgccgtatt ttgtgtaatt gcaggtattt attttattat ttactatcac tttcacgtcg
     2041 atgaaaatca atttggcgca atgggtgatt catttggcgg tttcgtgggc ccagttgcag
     2101 cttattttgc agcttatttt gcctatagaa catatcaagt acaaaaagca gacttaaagg
     2161 ctacaactga tggactaaaa gaggagcgag ataataaaaa aattcaagaa gttaaaattg
     2221 caatacagga gaaaattgat acaattgcag attcttttga gttcttaaaa gaaaatgtgg
     2281 atcggcttta ttacaaatct gtaaacaaag aagtatttga aggagttgct gctattgagc
     2341 agatatttat tgacctcaat aaacaatctg gaccgactca tttaatggca aaacaaactg
     2401 tcaaaagtct agaagtatgt aatgaaatat ttaattataa tacagaaaat acgatggctt
     2461 ttaacgataa caaggaaatg caggttctgc tcatttggga gctaatactt atttacgatt
     2521 ccatgtttta cacaacgtac gaaaattata atatgaagca tttgtttgaa acgtcaataa
     2581 cgacaataaa aaaattacgc aattccggcg attattcaaa cacgcagtgg ttaaacgaac
     2641 agggtcttac ggatttgtat gattctgtaa accttgctag ggctaattat aaaaatttat
     2701 cattcgcatt taatcaattg cgcagaagac aaattatgtg aaatgttact ataatactct
     2761 agctaaatat agctatcttt tctgaagtta cttttgtttt ttatatgcaa aaaatacaat
     2821 cactccacaa aggtattatg ctcggaacat atttttcagg aactacggca taaacacaaa
     2881 agccagcgct caaaaagcca gcgctttatt ccgttcctcc tgaaaaatat gttccggaca
     2941 taatagtcaa gttcctttct ttttattttt ttctcttttt tatttttctt ttgaaactat
     3001 ccgattagga taaagttatc agtacatgca aattaaaaac ccgactccat ctagcgactt
     3061 tgcctggtat ccccatgaaa acacaagatt tatagtaatc tattctttta taaatttcag
     3121 gctagcttct ccgtctgtcg ttttgacttt taataagtaa ataccagaag taaatgttct
     3181 tacatcaatt tgtataagtg atgaaacaat agtttgtgtc cacaaaattt gccctgcgat
     3241 gtttatgacc tcaataatag atccgtcaaa tcctgtatat tctacatata aaatattatc
     3301 agttgggttg ggatatattt taagtgtatt taaaatactt tcactgacac ttaatccctc
     3361 gtcaattaat agattgcaat catcatccag accatttagt atttcatctg ctccgggata
     3421 gatgttaggg ttggtatcat cgcaatctgc atcatcttct acaaagccat cgggaattga
     3481 gcaagaaagt gagtcaacat ttatatttcc aaagtcgtct ccgtctgcat cttcaaagga
     3541 atggatatag gttaggttat catcaacaaa gccatcgcaa tcgttatcta aatagtcaca
     3601 aatttcaatt gctcccggat aaattaaatt atttgcatca tcacaatcag ttgaatcact
     3661 tacatatcca ataaatacgg ctaagcaaga attaataaat atatcagaat taccaaagga
     3721 atctccatct tcatctttgt ataatgtgta agttgttaat ccctcatcta tatcgaaatc
     3781 gcagttatca tcaatgtcat tgcagatttc actagcagca ggactaattt cgacatttaa
     3841 atcattgcaa tcctcactgt taattacata accttcaagt tcaaaacatg caattgagtc
     3901 attgaatgta tcaccaaaat catcaccatc aacatctgca aaataggtaa caaatatcgc
     3961 atcttcgtca attaagccat tgcaattgtc atcgttggta ttgcaaatat cttcagcaga
     4021 aggataagtg aacgggtcgt catcattaca atcactgctg tcgccaacat atccaatagg
     4081 taaattgcat gaaagtgaat catttagtaa atcaccatat ccatcctcat cactatctgc
     4141 ataaaataaa gtaggcgtac aggatgcagc gagtttaact acccaaaaat cccaattatt
     4201 tccaattcca ttgtgatcag ttacatctcc atccgttgat ctagcccagc cagttactat
     4261 atacccctcg tcagaggctt gtgtgataga taatgcttcg tctgtgttag aaccaccgag
     4321 cgatttttcc caaataatat ttccattaga atcgatattc acaatccaat aatctgaata
     4381 tcctgctagc ccgtggtgcc cggtcacatt tccatcatta gaacctgaat gacctgcgag
     4441 tatataagag gtgtcaattg ctctataaat agaaaatcct tcttcagaag aactaccgcc
     4501 aagacatttt tgccacatta aaacacctag gctatctatt tttataaccc aataatcaga
     4561 aacgccacca tgaaagccag aaatatcacc attattagat tgggtgtatc ctaaaataat
     4621 atacccacta tcagcagtat tacttacact gaaagcatct tcatagtttg tgcctccata
     4681 tgctttttgc cactgtaaaa ccccatcttc agttatttta atcacccaaa aatcacctaa
     4741 accgtgattt ccgaaaacat cgccgttatt tgaagaagaa tggcctacta ttaaaaatcc
     4801 accatcaagt gttttgatgg cagactgcgc ttgatcatct tctgtcccgc cgtaacattt
     4861 ttgccaaact aaatctccat ctgaatttat ttttaaaatc caatagtctg cttctccatg
     4921 attaccactc acatcgccat tatttgatga agataaacct gtaattaaat atccaccatc
     4981 atctataatt gttactgaat tacctcgatc tgaggatgtt ccgccataag atttctgcca
     5041 ctgaatgttc ccaattgaat caagtttaat aatccataca tcatctgccc cattgttctc
     5101 acttaggtcg ccatcagtcg aagttgaaaa ccccacaata ataaatccga gatcttgtgt
     5161 ttgtttaatt tgaaaggctt gctcccaacc actgccacca taagattcct cccattcaat
     5221 ctcacctata ctatccaatt ttgcaaccca aatatctcct gatccggcat actcggtcac
     5281 gtcgccatca ttagaccaag tatcaccagt aacaataaat ccgcctccaa tgtcttcgca
     5341 aatgctatat gaggcatcat aacctgagcc tccgagcgct ttctgccaat gaatttcagg
     5401 cacttgggcg aagatacaac tttgaaaaat caggcataaa gcaaaaggag taactaaatc
     5461 tttcacaatt ggagttttga caaatataat taaaatataa ggaatacaaa aaaggtgtat
     5521 atttacctta aaaatgaaaa cacctttctt ctatataata ttttctattt tttgttgaac
     5581 acatatcaat taaccattta attaatttac atgagaacta aatcaaaaca tttattcaac
     5641 ttggaaaaac ttgcaaaagt ttttcttttt aatttaagtt tatttatttt tttgtttcaa
     5701 ggtaatttat taaaagccga aaattatttt tataccaatt atttatttga accaaatatt
     5761 ataaataact gtctcccaga ttcaacattt gatgacagtc tgaatgtata ttcagatgga
     5821 gatacagtaa gtcttttcac ttcaggagca gatacattaa ttgtacttta tgcatctgaa
     5881 agtgctgaca ctaattcttc ggcaaaactc attccttttg caacagaaga tatggttatt
     5941 agcattaata gtaataatca acctgttcag cacggtctgt atggaataaa tgtatcagat
     6001 ttatttgaac acgtaggcga attcacacca gatgggacac ctaatcccta ttacttacaa
     6061 tcgtgggatg atatagctag attggatcca gcagtaatca gagcattcag tgggtcaggt
     6121 agtaaattta tgcatccttt aggatctgac aacactgatc ctcttaatcc ttttgttatt
     6181 tctggactaa caagaaatgg aggttatggt tattgcatcg aagaaattat taattattac
     6241 gaaaatactg acggaacacc atttgatgcc ccacctctta atttgattat agcagatatg
     6301 ggattaccta cacccgttct aagctggatg aatcctaatg aaaaaggtgc atttaaagag
     6361 tactataaca aatggaacaa ccaaccacat tttgatccaa ctgttattcc tgacattgaa
     6421 gatcaaccgc tttatattaa tcaattaatt agattggttg cacaaattga aacatttaat
     6481 actctttcag gtaaagtcga tgttatactt gctataaata ttttaagcca aacagtaaat
     6541 gagataaagg atttgcttaa ttatttagtt gttgataatc caatttggcc tgttcaagtt
     6601 gtaggaatag aattaggtaa tgaaagttat tttccatcgc ataaccgatt aattggcttt
     6661 tctgattttg accattattg gtattttata aacggcggaa atgattatca gactgaattt
     6721 agtgacccat tagatttcga gttgacagat gtactaagcc tgacaatgca agcagaccat
     6781 gattttattg gcgacttgcg ttcacttatt ccgtttgaat ttaaaattgg aattccagcc
     6841 atgaatttac caaacggggc aggttgggcg tttaaaaccg ccaaccctga agacacttta
     6901 tatcctcctg taggtaaccc tgaaggattt tctccttgtg aactttcttg gaatcaatgt
     6961 cttgctttac actatgatga aatggttggg gaggattacg catttgatgc cgttattttg
     7021 catccttact atactccaag taatgaccca gcagctgggt ttggaacaaa ttgggggcgg
     7081 attccaacaa attacgatgt tgatccttta aatctaaccc caaaccattt ggataacgat
     7141 attttaactg acaatggata tcaagatttt gtggctccca tgtatgatta cacaacaccc
     7201 gacacaagac tacagaccgt atttgaaggg atggtaggta ttcctatttc agatgatgga
     7261 ggtctcacat attatgaggg aactggtaat ataaaagctt taataaggac acgctataaa
     7321 gaaagttacg atgaacatgc tggcatcctt aattttcagt cagctgatgt aggtcctcaa
     7381 tacaaggaat tatggacaac agaatggaat tttcatgatg cagatcattt agattttctt
     7441 cctattggtt atttaggtgc aaaccagatt agtgaacgat tggctgttta tacgaatact
     7501 cttgttcact tggatttagt acaagagtgg tatttaaaaa atttaaaaat gaatctccaa
     7561 cccggttatc gacataactt ttttacatat tcaacaattc aaaatattgc aggtggaagc
     7621 aatataacta tgcttagtga aagcaagcaa caagaccaaa ttcaaaaggg tttaatcgcg
     7681 agttgtgccg aaattgatct gttaccagat tattatataa agaaaggttc atattatggt
     7741 ttttctttat taaaaacaat ttatgagaaa aatctctatt atatttcttc cacaacctca
     7801 atgtatattg ctaacaacaa ccttgcgccg accgttttct tagcaccctc tgagaaaaag
     7861 ttatttattt attatacaaa cattaaaaat attcctcaac ggtatataat tgaccctaac
     7921 aatttagttt ccgtttaccc aggtgcttta ggagtttctt tcagcgcacc gtctatttta
     7981 tgtgtagatc ctaaacaatt atattctact tctggtagat cttctatttt agaaaacata
     8041 acgaaccaat atccttgcac aactgatatc acagactatc cagattggtt tgatttaaaa
     8101 agtgctgcct caataccagg ctttgctttt gtgtcatgtc ctccaggagt tcccgcggat
     8161 tgcaagtgcg ttgaagtgcc tgctacatca gttggctacg ttgaaatacc atttactcta
     8221 cttcgtttag gagagttaga agatatttat cgaatttacc caaatccgac ttctgaatat
     8281 ttcatcgttg aattagttac tccatttgaa ttggaaaata atgattttca ggttgacatc
     8341 ctaagtatga ctgggatgct tataaaatca gtacttactg gagagggcca aaaaattgat
     8401 attcaagata ttccagtggg agtttacaat gtaatgataa gaaaagaagg attcgctgtt
     8461 gagtcggaag tgcttgtgaa aatgaagtaa aaatagattt gtatgatatt taaaaggaac
     8521 ccgataaatt ttgtttcggg ttccttttaa atttaacact tgttaattcc tttaaataaa
     8581 aagaaaaaaa taacacaatt taaggtatac tctaatttgt cttaataggc atttgcagta
     8641 agtaatatct gaattacaaa atacaatcac ccacaaaagt attaggtttg atttattttt
     8701 atcaggaatt tcccggcata aacacaaaag ccgccgcact attgccagtg ctttattccg
     8761 ttcctcctga aaaatatgtt ccggacataa tgagcaagtt cctttctttt tatttttttc
     8821 tcttttttat ttttcttttg aaaatataca ggtagagata atctgtagct ctggtttaat
     8881 gcttatggtt gcaaaagctg agagtttcat tgaataacaa ccttttgaaa ttgatgtgta
     8941 gttgccgaag atgaggtata taaaatgtaa gtgcctggcg ggtacaatga tatatcaatt
     9001 tctacatcat tttgaatatt tgcagtataa aaaactgttc cttccatatc gtaaatatta
     9061 acagtaaccg gaattactgt attgttttgt atgagtcgta atttcagtaa ttctttaaca
     9121 atttgtggct caaaaattat attacttgaa gccggtcttt tgatttcaat aggaattgga
     9181 atgtgagcag tttctgattg tatgggaagt tctttgaaat tccactccgg aactatcaaa
     9241 tcttgtatga ttgattcagt ttcaagtcca ttttggtttg caggtatttg catttcgaca
     9301 tttgttgatt gttgtttttc acggatgaat tccatgaatt cactgtttaa ggtttgtccg
     9361 aatgcattaa tattaataca aaagtaaatt gagaagagta tgccttttat aaagatgcta
     9421 gttatttgt
//
LOCUS       DAORKW010000079         9419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_178_length_9419_cov_5.630607, whole genome shotgun sequence.
ACCESSION   DAORKW010000079 DAORKW010000000
VERSION     DAORKW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178_length_9419_cov_5.630607"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLB46_04850"
     CDS             complement(<1..445)
                     /locus_tag="PLB46_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRF75878.1"
                     /translation="MQLTTIKTLGELKKAGYTSLSVKEELRKNLIIKLRSGAPFFEGI
                     HGFEHTVIPDLERAILSRHNLILLGLRGQAKTRIARLLTTLLDEYIPVVSGSDLNDDP
                     FQPLSFYAKNLIAEKGDDTPISWMHRDERYTEKLATPDVSIADLIG"
     gene            complement(482..1576)
                     /locus_tag="PLB46_04855"
     CDS             complement(482..1576)
                     /locus_tag="PLB46_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRF75879.1"
                     /translation="MLGNRFSQFTEKPQSTFDTLFELLQELLVYTSGDMTEAMDWLNQ
                     LDRQYQITTPEYGMGDFIQELKDRGFLKDDEQEGGVLQITAKMEQSIRKSSLDQIFGK
                     LKKSQQGSHKTSHTGTGDENSTDMRNFSFGDAHEQIDYNESLKNAYINHGIHEFQMAE
                     DDLVVVEKAFKSQTSTVLMIDISHSMILYGEDRITPAKKVAMALSELIKTKYPKDTLD
                     VIVFGNDAWQIEIKDIPYLQVGPYHTNTVAGLELAMDLLRRRKNPNKQIFMITDGKPT
                     CIVRNGKYYKNSFGLDRIIVNKTLNLAAHCRKLGIKITTFMIAQDSYLQQFVEEFTQT
                     NQGKAFYTSLKGLGDYIFEDYERNRRRNVR"
     gene            complement(1582..2622)
                     /locus_tag="PLB46_04860"
     CDS             complement(1582..2622)
                     /locus_tag="PLB46_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRF75880.1"
                     /translation="MKYKLLGKSGLRVSEICLGTMTFGEEWGWGANFEESKKQFFGFL
                     EAGGNFIDTANRYTEGTSERFLGDFITESGRREDLVLATKYALVTHDRGLNLAGNHRK
                     NMIQSVEGSLKRLKTDYIDVLYLHAWDFTTYSEEVLRALNDLIQQGKIFYIAISDTPA
                     WIVSRANTIAELRGWNAFIGMQLEYSLLQRTGERDLIPMANAFDMGIIAWAPIAGGAL
                     TGKYLQQTDEAKRLQPNSARLNEKSRAIAQEVVNIAAETGFSAAQVAIRWVMQQHQQV
                     IPIVGARHLGQLNDSLGATSIQLQTEHLHRLNEISKIELGFPHDFLKGDGVRDILFGG
                     KLGELEIHHRGY"
     gene            2736..2867
                     /locus_tag="PLB46_04865"
     CDS             2736..2867
                     /locus_tag="PLB46_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75881.1"
                     /translation="MIKHFIAGGLSLLSMAVQAQQTDKQGFAKFVEEPWLVGEVYDN"
     gene            2922..4100
                     /locus_tag="PLB46_04870"
     CDS             2922..4100
                     /locus_tag="PLB46_04870"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRF75882.1"
                     /translation="MAVRWSYELMQSYDLDSVWLQEVMVPHWVRGAKEYGEVINFGQV
                     NMLALGGSIATPKEGIIEQVIEVKDFDELKALGAEKIKGKIVFYNHIFPQNVVNSFDG
                     YGEAGPYRWRGASEASKLGAKAVIIRSVGSAHDDFAHTGSTGFADGVKKVPCAALSAV
                     DADFLHDALIKDPALKFKMVMNCEMLDSVKSYNVIAQITGTQFPNEIIVVGGHLDSWD
                     VGEGAHDDGAGCTQSLEVLRTLKAINAQPKRTIRCIFYMNEENGNMGGKTYGAYAKGT
                     NKEKHIAALESDAGGFSPRGFNIDTTFLSHPKFDELVELMTFYGAYKFEVGHGGTDIG
                     PLEGAGTKLFGLSPDSQRYMDLHHTENDTFDKVNKRELHMGASVMGMLCWWLSEYGVK
                     "
     gene            4150..6447
                     /locus_tag="PLB46_04875"
     CDS             4150..6447
                     /locus_tag="PLB46_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF75883.1"
                     /translation="MKQLTQLLFLLCLLCVAQITLAQTVGGKIIDAQTKQPITGVTVV
                     SENNTGVVSDLDGNYQLALSPGNHTVTFSFIGYQKVTRNIQINEGTALLLPISLTAQF
                     AALDVVVVSGSLYAKKLSEETQSIEVIKSGLISNTNSTNLSDAITKAPGVYMMDEQAN
                     IRGGTGFTYGAGSRVMLVVDDQIMLAADRGDAKWNFVPMENVEQIEIIKGASSVLYGS
                     SALNGVIAVRTAWPKLEPESNITIYHGIYDKPAIKEAAWWDNAPTNTGINFSHKQKFE
                     KMDLVVGGHISQNNSHLLGQYSDRARLNWKTRFHPEQHKNITWGVNGNVMSDREGLFF
                     LWEDWDTAGFKPFQGYLDTSTTTLLNWQFKWMSVDPWLNYYDRFKNEHHFKMRFYNNS
                     VDYSDSTNGDAYLVNLDYQFHRSFKHDIVITAGTQGYYFNVRDYELGDHSGISAGVYF
                     QADKSLFDKLILNVGVREEFYQLDTSAGSAVPIFKGGLNYKAGQLTNIRLSFGQGYRF
                     PSLVEKFAKSKLGALFIFPNPDLVAEYGWNAELGIKRAINGDVIKGYADIAFYVTDYY
                     DMTEFTFNFYPGEGAGFKSLNTSRARIGGMEFTLGGEAVLGNGKLNWLGGYNYIYPAD
                     IAGDSSNLDVGTFMQNFIDGFTAKSTDSVFLSAVLKYRFRHMIKMDVEYAIQKWMFGA
                     DINYFSLMENLDAIYISFIPGITEYREGHFDGDWIADFRVGYYIKPDMRLQFIVKNAF
                     NNMYALRPAKYDPPRNFTVQYKIDF"
     gene            complement(6953..7195)
                     /locus_tag="PLB46_04880"
     CDS             complement(6953..7195)
                     /locus_tag="PLB46_04880"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRF75884.1"
                     /translation="MNFLYFILIGALAGWAAGKLTKGESFGLLGNIIVGIVGGVLGGW
                     IFNQLGIMKENLFYSLAAAVVGSVVFLLVIGLFRRK"
     gene            complement(7229..9040)
                     /locus_tag="PLB46_04885"
     CDS             complement(7229..9040)
                     /locus_tag="PLB46_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRF75885.1"
                     /translation="MAERRNKYAPVEPEDKKRITKESLKVAVKILRFLKPYKTPFIIG
                     SVFLLLSSLTTMTFPYFMGKLIDSAKNANPMPWMNNTNTMALILISILLLQSVMSFFR
                     VYLFATASERAMADVRMSFFDKMLTLGLPFYEQRRVGELTSRSANDVDQLQDMLSATL
                     PEFIRQLVTIVFGISFIFFISTPLTLLMISTFPVLVIMAVLYGKFIRKISKDRQDALA
                     NSNVVVEETLQNINTVKSFTNENYESKRYRTFMQRVVETGVKGAVNRGGFTTFLILGL
                     FGGFVLVIWYGTTLLKQGAILEGELFSFVLYTGFIGGAVGGLGDLYGRLQKTVGASER
                     IFDILEEEGEFDLTKTPETKVKLTGDIQLENIKFSYPTRKDITVLNGISIHINHGDKI
                     ALAGYSGSGKSTIAQLIMQMYTPDSGTIYFDGKPGNTFERRQLRYNMAVVPQEVLLFG
                     GTIKENIAYGKPNATNEEIIEAAKKANAWQFIAQFPEQLETVVGERGVKLSGGQRQRI
                     AIARAILKDPAILILDEATSSLDSESEKLVQDALEILMENRTTIIIAHRLSTIRNVDT
                     IFVLNNGSIVETGNFEALSKIENGVFNNLLKLQFNEN"
     gene            9108..>9419
                     /locus_tag="PLB46_04890"
     CDS             9108..>9419
                     /locus_tag="PLB46_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRF75886.1"
                     /translation="MVKQVNITLPAFERGFHLIDTYINSACGALPQTGIMHVFILHTS
                     AGICINENADPTVLMDFETVFNKLVPEGMSYYKHDMEGDDDMPAHIKAVMAGSSVTIP
                     IT"
BASE COUNT         2840 a         1685 c         1848 g         3046 t
ORIGIN      
        1 caccaattaa atctgcaata ctcacatcag gtgttgccag tttttcagtg taacgctcgt
       61 cgcggtgcat ccaactgatt ggtgtatcat cacctttttc tgcgattaaa tttttagcat
      121 aaaaggagag gggttgaaat gggtcatcgt ttaaatcgct gccgcttact accggaatat
      181 attcatcgag caacgtagta agtaaacgcg caattctggt tttagcctga ccgcgcaatc
      241 cgagtaaaat taaattgtga cgcgataaaa tggcgcgctc caaatccggt attaccgtat
      301 gttcgaagcc atgaatgcct tcaaaaaatg gtgctcctga acgcaatttt ataatgagat
      361 ttttgcgtaa ttcttctttt actgataatg aagtataccc ggcttttttt aattcgccta
      421 atgtttttat tgttgttaat tgcataaata tggtaatgtg aaattttaat aaacctgttt
      481 gttatcgaac gttacgtctt ctgttgcgtt cgtagtcttc aaaaatataa tcgcctaatc
      541 cttttaatga agtgtaaaat gctttgccct gattagtttg tgtgaactct tctacaaatt
      601 gttgtaaata actatcctga gctatcataa atgtggtaat ttttataccc aactttctgc
      661 aatgtgctgc aaggttgagt gttttattta caataatccg gtctaaccca aaactgtttt
      721 tgtaatattt cccattgcga acaatgcagg ttggtttgcc atcggtaatc ataaatattt
      781 gtttattcgg attttttctt ctccgcaata aatccatcgc aagttctaat cctgcaactg
      841 tgttggtatg ataaggtcca acttgtaaat aaggaatatc tttaatttca atttgccatg
      901 cgtcgttacc aaatacaata acatctaaag tatctttcgg atatttggtt ttaattaatt
      961 cacttaatgc cattgctact tttttggccg gtgtaattct atcttcacca tacaaaatca
     1021 tggaatgcga aatgtcaatc atcaaaaccg ttgaagtctg cgatttaaat gctttttcta
     1081 caaccactaa atcatcttcc gccatttgga attcatgaat gccatggttg atatatgcat
     1141 ttttgagcga ctcattataa tctatttgtt catgcgcatc tccaaaacta aaattgcgca
     1201 tatcagttga attctcatcg ccggttccgg tatgtgaagt tttatgactg ccttgttgtg
     1261 atttttttaa cttgccaaaa atttgatcga gtgaactttt gcggattgac tgttccattt
     1321 tggcagttat ttgcaatact ccaccttcct gttcatcatc cttcaaaaat ccgcggtctt
     1381 ttaattcctg aataaaatca cccataccat attccggagt ggtaatttga tattgcctgt
     1441 cgagttgatt taaccaatcc atagcctcgg tcatatcacc actggtgtaa accaataact
     1501 cttgtaacaa ttcgaaaagg gtatcgaatg tggactgtgg tttttctgta aattgtgaaa
     1561 atctatttcc gagcataatt attaataacc tctgtgatgt atttccagtt cacctaattt
     1621 gccgccgaat aaaatatctc ttacgccatc tcctttcaga aaatcgtgag ggaagcccaa
     1681 ttctatttta cttatttcat taagtcggtg taaatgttcc gtttgtaatt gtattgaagt
     1741 agctcccaga ctgtcgttta attgtccaag atgtcgtgct ccaacaatag gaattacctg
     1801 ctgatgctgt tgcattaccc atctgatggc aacctgagca gctgaaaagc ctgtttctgc
     1861 ggcaatatta acgacttcct gagcaatggc gcggcttttt tcatttaatc ttgcgctgtt
     1921 gggctgcaaa cgttttgctt catcggtttg ctggagatat tttccggtta aggcaccacc
     1981 tgcaattgga gcccaggcaa tgatgcccat atcaaaagca ttggccatag gaattaaatc
     2041 gcgttctccg gttctttgca gaagcgaata ttccagttgc ataccaataa aagcattcca
     2101 accgcgtaac tcggcaatgg tatttgccct cgacacaatc caagccggcg tatcagaaat
     2161 ggcgatataa aatattttcc cctgctgaat caaatcgttt agtgcgcgta atacttcttc
     2221 actataagtc gtaaaatccc atgcatgtaa atacaacaca tcaatgtaat cggttttgag
     2281 acgtttcagg ctgccttcaa cactttgtat catgtttttg cggtgattgc ccgctaaatt
     2341 caaccccctg tcgtgcgtaa ccaaggcata tttggttgcc agcactaaat cttcacgacg
     2401 accgctttcg gtgataaaat cgcctaaaaa gcgttcactg gtgccttctg tgtatctgtt
     2461 ggcggtatca ataaagttac ccccggcttc aagaaagccg aaaaattgtt ttttactttc
     2521 ctcaaaattg gctccccagc cccattcttc gccgaatgtc atagtgccta agcagatttc
     2581 ggagacgcgt aaaccgcttt tgcctaataa tttatatttc atatttggat gtttttggta
     2641 attcggtttt taaggttgct aacaagattg ttactttaaa acttaaatga actgaatagg
     2701 ttcattattt tagagcaaaa ttcaaattta atataatgat taaacatttt attgccggag
     2761 gattaagttt gctttcaatg gcggttcagg cacaacaaac cgacaaacaa ggatttgcaa
     2821 aatttgtcga ggaaccgtgg ttggttggtg aagtttatga taattgacat gatgtatgaa
     2881 aaagtaaggg actgcgatgg gggggttcac cacaagccga tatggcagtt agatggagtt
     2941 atgaattaat gcaaagttat gatttagatt cggtgtggtt gcaggaagta atggttccac
     3001 attgggtgcg cggcgcaaaa gaatatggtg aagtaattaa tttcggtcag gtaaatatgc
     3061 ttgctttagg tggcagtatt gcaacaccga aagaaggtat tattgaacaa gttattgaag
     3121 taaaagattt tgatgaatta aaagcattag gtgcagaaaa aataaaaggg aaaatagttt
     3181 tttataatca tatttttccg caaaatgtgg tgaattcatt tgatggttat ggagaggccg
     3241 gaccttatcg ctggagaggt gcgagtgaag caagtaagtt aggtgcaaaa gctgtaatta
     3301 tcagaagtgt gggttctgca cacgatgatt ttgcacatac aggtagcacc ggttttgccg
     3361 atggtgttaa aaaagtacct tgtgctgcat taagtgctgt tgatgctgat tttttacacg
     3421 acgcattaat taaagatcct gcacttaagt ttaaaatggt tatgaattgt gaaatgctgg
     3481 attcagtaaa atcttataat gtaattgcac aaattaccgg aacacaattt cccaacgaaa
     3541 taattgtggt tggcggacat ttagatagtt gggatgtcgg cgaaggtgca catgatgatg
     3601 gtgccggatg tacacaaagt ttagaagtgt tgcgcacttt aaaagccatt aatgcacaac
     3661 caaaacgtac cattcgttgt atattttata tgaatgaaga aaatggcaac atgggtggaa
     3721 aaacatatgg tgcttatgct aaaggaacca ataaagaaaa acatattgct gcattagaaa
     3781 gtgatgcagg cggattttca ccaaggggat ttaatatcga tactaccttt ttatcacatc
     3841 ctaaatttga tgaattagtt gagttgatga cattttatgg cgcctacaaa ttcgaagtag
     3901 gccatggtgg aactgatatt ggaccattgg aaggtgctgg cactaaatta tttggtttat
     3961 cacctgactc gcagcgatat atggatttac atcacacaga aaatgatact ttcgataaag
     4021 taaataaacg tgaattacac atgggcgctt cagtaatggg gatgttatgt tggtggttaa
     4081 gtgaatatgg ggtgaaataa taaagtccta aagcataatt ttttaccttt ataaaaacgc
     4141 agtattcaaa tgaaacaact aacacaactt ttatttttac tatgtttgtt gtgtgtcgca
     4201 caaattacct tagcacaaac tgttggtggt aaaattattg atgcacaaac taaacaaccc
     4261 attaccggtg taactgttgt gtccgaaaat aatacaggtg ttgtatctga tttggatggt
     4321 aattaccaat tagcattatc tcccggtaat catactgtta cattttcttt tattggatat
     4381 cagaaagtta ccagaaatat tcaaattaat gagggcacag cattattatt accaattagt
     4441 ttaacagctc agtttgcagc attagatgtt gttgttgtaa gtggtagttt atatgccaaa
     4501 aaattatccg aagaaacgca aagtatagag gtaattaaat caggattaat ttccaatacc
     4561 aattctacca atttatcaga tgctattacc aaagccccgg gcgtttatat gatggacgaa
     4621 caggctaata ttcgcggagg aaccggattt acttatggcg caggaagtcg tgtgatgttg
     4681 gtggtagatg atcagataat gctggcagct gatcgcggtg atgcaaaatg gaattttgtt
     4741 ccaatggaaa atgtggaaca aattgaaata ataaaaggtg catcgtctgt tttatacggt
     4801 agcagtgcat taaatggtgt aattgcagta agaactgcat ggccgaaact ggaacctgaa
     4861 agtaatatta ctatatatca cggcatctac gataaacctg ccataaaaga agctgcatgg
     4921 tgggataatg caccaaccaa taccggaatt aatttcagcc ataaacagaa atttgaaaaa
     4981 atggatttgg ttgtgggagg acatatttct caaaacaata gtcatttatt aggccaatat
     5041 tctgacaggg caagattaaa ctggaaaaca agatttcatc ctgagcaaca taaaaatatt
     5101 acctggggtg ttaatggtaa tgtgatgagc gacagagagg gattattttt tttatgggaa
     5161 gattgggata ctgcaggctt caaacctttt cagggatatt tagataccag cactactact
     5221 ttattaaatt ggcaatttaa atggatgagt gttgatccat ggttaaatta ttacgacagg
     5281 tttaaaaatg aacatcattt taaaatgcgt ttttataaca atagtgtcga ctactcagat
     5341 tcaacaaatg gtgatgcgta tttggtaaac ttggattatc agtttcaccg ttcatttaaa
     5401 catgatattg ttataacagc aggtactcaa ggttattatt ttaatgtgcg cgattatgaa
     5461 ttaggcgacc attcaggcat ttctgctggt gtttattttc aggcggacaa atcgttgttc
     5521 gataaattga ttttaaatgt tggcgttagg gaagaatttt accagttaga taccagtgca
     5581 ggttctgctg tgcccatatt caaaggtggt ttaaattata aagctggaca attaacgaat
     5641 atccgtttat cgtttggtca gggatatcgt tttccgagtt tagtcgaaaa atttgctaaa
     5701 tcgaaattag gtgccttatt cattttccca aatcctgatt tagttgctga gtatggatgg
     5761 aatgccgaat taggtattaa acgcgcaatt aatggtgatg tcataaaagg atatgcagat
     5821 atcgcatttt atgtgaccga ttattacgac atgactgaat ttaccttcaa cttttatccg
     5881 ggtgaagggg cgggatttaa atcgctgaat accagtagag ccagaatagg aggaatggaa
     5941 tttacactag gtggtgaagc agtattaggc aacggaaaat taaattggtt gggtggatat
     6001 aattatattt atcctgcaga catagcaggt gattcatcca atcttgatgt gggaacattc
     6061 atgcaaaatt ttattgatgg atttactgct aaatctacag attctgtttt tttaagtgcc
     6121 gtgttaaaat atcgttttcg gcacatgata aaaatggatg tggaatatgc cattcaaaaa
     6181 tggatgttcg gagctgatat taattatttt agtttgatgg agaatttaga tgccatctat
     6241 atctcattta ttcccggaat caccgaatat cgggaaggcc attttgatgg tgactggatt
     6301 gcagatttcc gtgttggtta ttacatcaaa ccggacatgc gtttgcagtt tattgtaaaa
     6361 aatgcattta ataatatgta tgcacttcgc ccggcaaaat atgatccgcc taggaatttt
     6421 acggtgcagt ataaaattga tttttaatta gctaattagc tgattagctg attagctaat
     6481 gggggttgat cgatgggata acagctatac ctattaccgg tggtttttcg aaattgtcga
     6541 atgcggatgc ttggcgcatc cttggtttat ttgcaattat attaagtcag cacgtgggtt
     6601 gacggctaca ccgctccgaa ttgtattttt tttaattcaa tttttctgct gctgcggaaa
     6661 aagcaattaa tatgcgtccg ccgcggcgga cacattagca catcattttc cggctgttta
     6721 attggtacat cggtacattt acacattggt acattaatcg gtttagcggg ctaatattta
     6781 ttagccccaa cggtagatct aaacgaaaac cgcagtcaaa atttccggct attcccgcca
     6841 cgattgatgc ttcgcattgg cacataggta catttacaca taggtacatc ggcacattcc
     6901 cacattggta cattaattag cacatcagca ccttatcaca tcagcacatt aatcacttac
     6961 gtctaaacaa accaatcact aataaaaaca caactgagcc tactacggca gcggcgaggc
     7021 tgtagaataa attttctttc atgatgccga gctgattaaa aatccagccg cctaaaacgc
     7081 ctcctacaat accaacaatt atattaccta acaatccaaa gctttcacct tttgttaatt
     7141 tacctgcagc ccagccggca agtgcaccaa ttaatataaa ataaaggaag ttcatagtgg
     7201 tttgatttta tgtttggtta tcgggttttt agttttcgtt aaattgtaat ttcaataaat
     7261 tattaaatac accgttttct atttttgaca aggcttcaaa atttcctgtc tcaacaattg
     7321 aaccattatt taatacaaaa atagtatcaa catttctgat ggtagataaa cgatgcgcga
     7381 taatgattgt tgtgcggttt tccattaata tttccagcgc atcctgtacc agcttttcac
     7441 tttctgagtc gagcgaactg gttgcttcat ctaatattaa aatggcaggg tcttttaaaa
     7501 ttgctctggc aatcgcaatt cgttgtcgct gaccgcctga taattttacg ccgcgttcac
     7561 ccacaactgt ttcgagttgt tccggaaact gcgcaataaa ttgccatgca tttgcttttt
     7621 ttgccgcctc tattatttct tcatttgtcg cgttaggttt gccataagca atgttttctt
     7681 taatggtacc gccgaataat aatacttcct gtggcactac tgccatatta tagcggagtt
     7741 gtcttctttc aaaagtattt ccaggtttgc catcaaaata aatggtaccg ctgtcaggcg
     7801 tatacatttg cataattaat tgcgcaatgg ttgattttcc tgaaccacta tatcccgcca
     7861 acgctatttt atcgccatga ttaatatgaa tactgatgcc atttaatacg gtgatatctt
     7921 ttcttgttgg gtatgaaaat ttaatatttt ctaattgaat atcgccggtg agtttaactt
     7981 tagtttccgg tgttttagtt aaatcaaatt caccttcttc ttctaaaata tcaaaaatac
     8041 gttctgaagc accaactgtt ttttgtaatc gtccgtataa atcgcctaac ccaccaacgg
     8101 ctccaccaat aaatccggta tataaaacga agctgaataa ttcaccttct aaaatcgcac
     8161 cttgttttaa taaagtagtg ccataccaga ttactaaaac aaatccgccg aataaaccta
     8221 aaattaaaaa ggtagtaaat ccgcccctgt tcactgcacc ttttacgccg gtttcaacca
     8281 cacgttgcat aaatgtacga taacgttttg attcgtaatt ttcgttggtg aatgatttta
     8341 cggtattaat attttgtaat gtttcttcaa caacaacatt tgaatttgct aatgcatcct
     8401 gacggtcttt cgaaattttg cggataaatt ttccatacaa tacagccatg attaccaaaa
     8461 ccggaaaagt ggaaatcatc aataaagtaa gtggtgttga aataaaaaat ataaatgaaa
     8521 taccgaaaac gatagtcacc agttgacgaa taaattccgg taacgttgca ctgagcatat
     8581 cctgtaactg gtctacatca tttgcggagc gactggttaa ttctccaact ctgcgttgtt
     8641 cgtaaaaagg taatcccagc gtgagcattt tatcaaaaaa cgacatccgt acatctgcca
     8701 tagccctttc cgatgctgtc gcaaataaat acactctgaa aaaagacatc accgattgta
     8761 gcaataaaat actgattaaa atgagtgcca tggtattagt attattcatc cagggcatgg
     8821 ggttggcatt tttagcagag tcgatgagtt tccccataaa ataagggaag gtcatggtag
     8881 tgaggctgga taacaataaa aataccgagc cgattatgaa gggtgtttta taaggtttga
     8941 ggaagcgtaa aatttttacg gcaactttca gactttcttt ggtaatgcgt tttttatctt
     9001 ccggttcaac gggagcatat ttatttcttc tttctgccat aatgggtcac aaaattatcc
     9061 tataaacagg tacagacggc ttaaagttgt aataattttg cagggagatg gtaaaacaag
     9121 taaatattac actgcctgct tttgaaagag gttttcactt aatagatacc tatatcaata
     9181 gtgcttgcgg agcattaccg caaaccggta ttatgcatgt ttttattttg catacttcag
     9241 caggcatttg tattaatgaa aatgccgacc caactgtttt gatggatttc gagaccgtat
     9301 ttaacaaact ggttcctgaa ggaatgtcct attacaaaca cgacatggaa ggcgatgacg
     9361 atatgcctgc acatattaaa gcagtaatgg cgggttcatc ggttactatc ccaattact
//
LOCUS       DAORKW010000080         9330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_185_length_9330_cov_6.590512, whole genome shotgun sequence.
ACCESSION   DAORKW010000080 DAORKW010000000
VERSION     DAORKW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9330
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185_length_9330_cov_6.590512"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..611)
                     /gene="iscU"
                     /locus_tag="PLB46_04895"
     CDS             complement(192..611)
                     /gene="iscU"
                     /locus_tag="PLB46_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HRF75887.1"
                     /translation="MAYSKKVLEHYENPQNVGTLDSKKNEVGTGLVGAPECGDVMRLQ
                     IEVDPATQKIVDAKFKTFGCGSAIASSSVATEWLKGKTIDEALTIDNMDIVEELALPP
                     VKIHCSVLAEDAIKAAIQDYRKKNNMPELQLEEKIVH"
     gene            complement(696..1913)
                     /locus_tag="PLB46_04900"
     CDS             complement(696..1913)
                     /locus_tag="PLB46_04900"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HRF75888.1"
                     /translation="MLKLPIYLDNSATTPADPRVVDTMVPYFYEHFGNAASRSHPFGW
                     EAEDAVDQARERIGKLIGATDKEIIITSGATEADNLAIKGVYDMYSRKGNHIVTVTTE
                     HKAVLDTCKHLEKQGAQITYLKTDAEGNIDLNELESMVTDKTIMVAVMYANNEIGTIA
                     PIRQIADIAHKKGALMFSDATQAAGKISIDVDRDGIDLMAFTAHKMYGPKGVGALYVR
                     RKNPRVKVTAQMDGGGHERGMRSGTLNVPGIVGFGKAAELAMMEMEIDAARLSVMRDR
                     LENELCKIEESYVNGNRRSRLPHITNISFKYVEGEGLMMAFNQNIAVSSGSACTSASL
                     EPSYVLKALGLGDDLAHSSIRFSLGRFTTDEQIQYTIDHVTKAVNKLRDMSPLWEMFK
                     QGIDLNSIEWAEH"
     gene            2145..2549
                     /gene="mce"
                     /locus_tag="PLB46_04905"
     CDS             2145..2549
                     /gene="mce"
                     /locus_tag="PLB46_04905"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRF75889.1"
                     /translation="MKKIEHIGIAVKSLETSNELFAKLFGKEHYKIEAVEREGVKTAF
                     FMMGETKIELLESSNENSAIAKFIEKRGEGVHHIAYAVDDILAEMHRLESLGFQLLNK
                     EPLPGADNKLICFLHPKTTNGVLVELCMDKPL"
     gene            complement(2607..4454)
                     /locus_tag="PLB46_04910"
     CDS             complement(2607..4454)
                     /locus_tag="PLB46_04910"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HRF75890.1"
                     /translation="MRNFYVLILGLSSFAASATEPTANNYTATIDLTVVENDRVKVTI
                     DVPAITSDNIVYCIPKIVPGTYSIYDFGRFVFDFKAYNENGTALKVEPVDVNRYKIYN
                     ATTLKKIEYWIDDTYDMTGDNPIFEPAGTNIAPDNFVVNTFGFIGYLDGMKENDYELI
                     IKKPSGFYGSTPLKAKSAGTNLDKFHLDDYDHLADSPLLYAVPDTASTMVGNAQVLIG
                     VYSPNKVITAKQIMDNVSEILAAQGEYLGGKLPVDKYAFLLYFTDTTGVSGGMGALEH
                     KNSSVYFLPETAIENIAPMLRDVCAHEFFHVVTPLAIHSHEIADFNFIEPKMSKHLWL
                     YEGQTEYAAHHAQVKAGLITHEVFINRMQEKIETSTNYYNDTLPFTVMSLGCLDVYEE
                     QYGNVYQKGALINMCLDIELRQLSKGKYGTQELMRDLGKEFGANKAFDDDELFDKIAK
                     MTYPEIRNFFATYVEGNTPLPYEKYLNKAGIILSPEGTMDVISMGNISMNYNITPESS
                     EIIIVGLEGSNSFAKDMGYKIGDKFLSINGQSLTAGGANRVIDVWKETTQAGDKVVVM
                     VERKLENGKVKKVKLKGNAITVKVPMPRTLMLDDQATPEQVAFRKAWLG"
     gene            complement(4638..6293)
                     /locus_tag="PLB46_04915"
     CDS             complement(4638..6293)
                     /locus_tag="PLB46_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF75891.1"
                     /translation="MAIERTVHSPLATFYKWENAQRNRLYLRQPINGEWQTYTWGECG
                     DQVRRMAAYLKSLNYEPGSRIAILSKNCVHWMLSDFAIWLAGYVSVPMYPNMSPNTVR
                     QILEHSESKMLFVGKLDDWAYLKPGVPENLPCVAYPFYGITEYKNWDDIIKNNAPIKE
                     NPDYPMQNLCTIMYTSGTTGVPKGVMHTFYNFGFAAEYAFPELKVDNTAVFFSYLPMC
                     HIAERLLIEMGSLYTGGQVSFAESLETFPKNLQETSPTLFLAVPRIWAKFQEKILEKM
                     PQQKLNRLLKIPIVNGIVKKKIKAALGLSKAEQCFSGAAPISVDTINWFWKIGIKIQQ
                     AYAMTEDCCYSHTNYIDRNKIGTVGLRFPHVDVKISEQGEILIKHPAMMKGYYKEEAQ
                     TKEVFNEEGYFKTGDKGDVDSEGYLKITGRVKELFKTSKGKYVAPMPIEMKMAANTDI
                     GQALVIGYGLPQPMVLITLSENGKKKSNADVEAGLDKTLKAVNETLDSFEKLEKVVVM
                     KEEWTIQNNMLTPSLKIKRNEVEKLFQDNYEKWFEMKGDVIWQ"
     gene            6411..7454
                     /gene="thiL"
                     /locus_tag="PLB46_04920"
     CDS             6411..7454
                     /gene="thiL"
                     /locus_tag="PLB46_04920"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRF75892.1"
                     /translation="MSEQVNRTEISSLGEFKLIEQLTKNFELRNASSLKGIGDDAAVI
                     DNTNKLTVVTTDMLVEGIHFDMMYSPLLHLGYKCIAVNVSDLLAMNAEPKQVTVSIAI
                     SNRYSVEAMEELYEGIYHACEYYGVDLIGGDTTSSLKGLIISVTAIGEANEDEIVYRS
                     GAKDGDLICVSGDLGGAFLGLQLLEREKKIFLENPGIQPDLENKKHIVGRFLKPEARK
                     DIVEAMRAQSIVPTAMIDISDGLSSEILHICNQSNCGAIIYENEIPIHPESYDQALIF
                     NMDPTVCALNGGEDYELLFTIDPKHATAIAEIPNVSVIGKIVPQANGTFLKTKNDNLF
                     KIQAQGWESFKQE"
     gene            complement(7455..8786)
                     /locus_tag="PLB46_04925"
     CDS             complement(7455..8786)
                     /locus_tag="PLB46_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75893.1"
                     /translation="MPVLLNIMLRRISAITLILFHFTLDAQINCDNDSSLLIPIVDLQ
                     FDTYLGFQGGLYPGGLNVMPAAHADSGVVHANAIKPINFDGEIDTTYGKVVMVGLGNG
                     SAGKSFNKFIGEYLNQGYTDSCVRLINACMENYSLHDMISSDANDVYWKDVNDFFQNV
                     DLKKKQVSVVWMQAISFEDTFFTAEQYVDTLTNTYREVIRKLKDQFINLKLIYISGLH
                     YGGYSDTLGEHYTAVGEPAPYFNDFAIKAIIQQQIEGDTLLKYSGEDAPAAWVAWGPN
                     FWADGRTLREYDDLRWLCPGDFDTDQDGFLLSGSGQQKAADRLFTFFSTEPTSTPWFF
                     GLPYDCFTEIEAEDTTGVDSLIIPEDEILWITQNPVKGVIKFVINIDTQDKADILVCN
                     ALGQTIVEGAFYKVEKGRIFSINIEQHARGIYVLSVFVEGKVYNKLFYLDD"
     gene            complement(8808..>9330)
                     /locus_tag="PLB46_04930"
     CDS             complement(8808..>9330)
                     /locus_tag="PLB46_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75894.1"
                     /translation="IWGPPLWADGKNPRDYDGLTWNCEAEFAIDGGGYHLTNEGKDKL
                     ANILLDFFRTDTLSESWFMDGPKWASCGDGRLADGSIILPEETIITKEDITVFPNPSN
                     GQFYVDFDEVLTEAIQVKVVNQIGEVVMSESYGSVQPFSFYQFDLSGKPAGIYYFEVV
                     IGNQLFSQPIVLN"
BASE COUNT         2900 a         1839 c         1559 g         3032 t
ORIGIN      
        1 atcaaagtcc attttatagc tcaaacctga gcaaccgccg cccactacgc tgattcggat
       61 aaaataatcg tttcccaatc ccgcttcatt tttgagctgg tctactttct gttttgcgtg
      121 ttctgatata aaaatcataa acttaaatac aaggttaaac gctatgtgaa taacgtgttc
      181 cttaattgat tttagtgaac aattttttct tctaattgca gttcaggcat attatttttc
      241 ttgcgataat cctgaattgc tgctttgata gcatcttctg ctaatacaga gcaatggatt
      301 tttaccggag gtaaagccag ttcttccacg atgtccatat tatctatcgt taatgcttca
      361 tcaattgttt ttccttttaa ccattcggtg gctactgatg aagaagcaat tgcgcttccg
      421 caaccaaatg ttttaaattt agcatccaca attttttgtg tagcagggtc aacttcaatt
      481 tgcaaacgca tcacgtcacc acattcagga gcaccaacta atccggttcc cacttcattt
      541 tttttgctat cgagtgttcc tacattttgc ggattctcgt aatgttccaa cacttttttt
      601 gagtatgcca tattcagttg ttttctttat ttaatttatt gcctttaaat gataacactt
      661 gaactaagta aataattcaa attgatattt actatttaat gttctgccca ttctatggaa
      721 tttaaatcaa ttccttgttt aaacatttcc cacagcggac tcatgtcgcg taatttattt
      781 actgctttag ttacatggtc aatggtgtat tgaatttgtt catcggtagt aaaacgacct
      841 aatgaaaaac gaatacttga atgcgctaaa tcatcgccta aacccaaggc tttcaacaca
      901 taactaggct ctaaagatgc actagtacag gcacttccgg agctaaccgc aatattttga
      961 ttaaatgcca tcatcaaacc ttcaccttcc acatatttga aagatatatt ggtaatatgc
     1021 ggcaaacggc ttctgcgatt tccatttaca tatgattctt ctattttgca caattcgttt
     1081 tctaaacggt cgcgcatcac actcaaacga gctgcatcaa tttccatttc catcatggct
     1141 aattctgctg cttttccaaa tcctacaatt ccaggtacat ttaaagtacc actgcgcata
     1201 ccgcgttcgt gtccaccacc atccatttgg gcggtcactt taacacgtgg attttttctg
     1261 cgaacataaa gtgcaccaac acctttcggg ccgtacattt tgtgtgcagt aaatgccatt
     1321 aaatcgatac catcacggtc aacatcaata ctaattttac ctgcagcctg cgttgcatct
     1381 gaaaacatta atgcaccttt tttatgtgcg atatcggcaa tttgacgaat tggagcaatt
     1441 gttccgattt cattgttggc atacataaca gccaccataa tagttttatc ggtaaccata
     1501 ctttccagtt cgttcaagtc gatgttacct tctgcatcag ttttgaggta ggtaatttga
     1561 gcaccctgtt tttcgaggtg tttacaggta tccaaaacag ctttgtgttc ggttgtaact
     1621 gtaacaatat gattgccttt gcggctatac atatcgtaca cccctttaat agcaaggtta
     1681 tcggcctcag tagcacctga agtaatgata atttctttgt ctgttgctcc aattaactta
     1741 ccaattcgct cacgagcctg gtctacagca tcttcagctt cccagccaaa aggatggctt
     1801 ctgctggcag catttccaaa atgctcataa aaataaggga ccatggtatc caccacacga
     1861 ggatcggcag gggttgtagc actattatct aggtatattg gtagtttgag catatttcgc
     1921 tatttagact aattctaaaa cacaaagtta atagaaaagt tctaaacttg ggaaaaattg
     1981 cggctatatt cccaaacctg tttggctgaa acctgcaatt ggcctaaaat cccgcctttt
     2041 ttaggtttac attaagttta atgctagtcc aaacagtaga aaaattgcct gttttagcgt
     2101 catagtgtta atttggtaat gtaagtttgc ataaacacgc gattatgaaa aagattgaac
     2161 atattggtat tgcggtaaag agtttggaga cctccaatga gttatttgcc aaactttttg
     2221 ggaaagaaca ttacaaaata gaagctgttg aacgcgaggg tgtaaaaacc gcatttttta
     2281 tgatgggaga aaccaaaatt gaattattgg aatcgagtaa tgaaaattct gcaattgcaa
     2341 aatttattga aaaacgcggt gaaggtgtac atcatattgc ctatgccgtt gacgatattt
     2401 tagcagaaat gcatcgctta gaaagtttgg gatttcaatt attaaataaa gaaccattac
     2461 cgggagctga taataaattg atttgttttt tacatcctaa aacaacaaat ggtgtattgg
     2521 tggaattgtg tatggataaa ccgctataaa aaaagttgca atagttttaa ctaccgcaac
     2581 tttactttac actatcaaat atttatttaa cctaaccagg cttttctaaa tgctacctgt
     2641 tccggagtag cttggtcatc cagcattaat gttctgggca taggcacttt aactgtaatg
     2701 gcattgcctt ttaattttac tttcttcacc ttcccatttt caagcttacg ttcaaccatt
     2761 accacaacct tatcaccggc ttgtgttgtt tctttccaaa catcgattac cctgttagca
     2821 cctccggctg tgagtgattg tccgttaata cttaaaaatt tatctccaat tttataaccc
     2881 atatcttttg caaaactatt gctgccctct aaaccaacaa ttataatttc actgctttca
     2941 ggagtaatat tataattcat cgaaatatta cccatactta tcacatccat tgtaccttca
     3001 ggtgataaaa tgattcctgc cttattcaaa tatttttcat aaggcaaagg tgtgttacct
     3061 tccacataag ttgcaaaaaa attacgaatt tccggataag tcattttagc aattttgtcg
     3121 aacaattcat catcatcaaa tgctttgttg gcgccaaatt ctttgcctaa atcgcgcatt
     3181 aattcctgcg taccgtattt acccttgctc aattgtcgta attcaatatc caaacacatg
     3241 ttaatgagtg cacctttctg atatacattg ccgtattgct cttcataaac atctaaacaa
     3301 cccaaactca ttacagtaaa tggcaaagta tcgttataat aattagtgga agtttcaatt
     3361 ttttcctgca tacgattaat aaatacctcg tgtgtaatta atccggcctt tacctgcgca
     3421 tgatgtgcag cgtattctgt ttgcccttca tacaaccata aatgtttact cattttaggt
     3481 tctataaaat tgaaatcagc tatttcgtgg ctgtgaatgg caagtggtgt taccacatgg
     3541 aaaaattcat gcgcacaaac atcacgcaac atgggtgcaa tattttcaat ggccgtttcc
     3601 ggtaaaaagt aaacacttga atttttgtgt tcgagtgcac ccattccacc gcttactcct
     3661 gtggtatcgg tgaaatacaa taaaaacgca tatttatcaa ccggtaattt ccctcccaaa
     3721 tattcgcctt gtgcagcaag tatttcgcta acattatcca ttatttgttt tgcggtaata
     3781 actttattag gcgaataaac gccgattaat acctgcgcat tacctaccat agtagaagcc
     3841 gtatcgggca cagcatacaa caaaggtgaa tcagctaaat ggtcgtaatc atctaaatga
     3901 aatttatcta aattagtacc ggcagatttt gcttttaaag gcgttgaacc gtaaaatccg
     3961 gaaggttttt taataatcag ctcataatcg ttttctttca tcccatctaa atatccaata
     4021 aatccaaagg tgttaaccac gaaattatcg ggtgcaatat ttgttccggc aggttcgaaa
     4081 atgggattat cacctgtcat atcatacgta tcatcaatcc aatattcaat ttttttaagt
     4141 gtagtagcgt tatatatttt atacctgttc acatcaacag gttcaacttt taaagcagtg
     4201 ccattttcgt tgtaagcttt aaaatcaaaa acaaaacgtc cgaagtcata aatggaatat
     4261 gttccgggta caattttagg tatgcaataa acaatattat ccgaagttat ggcgggcaca
     4321 tcaatagtta ctttaacgcg gtcgttttca acaacggtaa ggtcgatggt agcagtgtaa
     4381 ttatttgcag ttggctcggt agcactggca gcaaaactag atagccccag tattaataca
     4441 taaaaattgc gcatacatgt taaattgtaa gccgaaaata actgtatgat ttattaagga
     4501 atgttaatgt taagagagat taacttaaga ataaaaaaat taacaacttt atctcgcaac
     4561 aaaattgaaa atagaaaaaa tataacgagc agggacaaca gttttccaat cacaaaaaca
     4621 attgccgcat catgaagtta ttgccaaatt acatcccctt tcatttcaaa ccatttttca
     4681 taattatcct ggaataattt ctccacttcg ttgcgtttaa tttttaatga aggtgtaagc
     4741 atattatttt gaatagtcca ctcctctttc atcactacaa ctttttccaa tttttcaaaa
     4801 gaatcgagtg tttcattaac ggcttttaat gttttatcaa gtccggcttc aacatcagca
     4861 ttagattttt tctttccatt ttctgataag gtaatcaaca ccattggttg tggtaagcca
     4921 taaccgataa ccagcgcctg tccaatatct gtatttgcag ccattttcat ttcgattggc
     4981 attggcgcaa catatttgcc tttacttgtt ttgaataatt cttttacacg accggtaatt
     5041 tttaaatatc cttctgaatc tacatcacct ttatcgccgg ttttaaaata tccttcttcg
     5101 ttaaatactt cttttgtttg tgcttcttct ttataatatc ctttcatcat agcaggatgt
     5161 ttaattaata tttcaccctg ttcactgatt ttaacatcaa catgcgggaa gcgtaaacct
     5221 acagtcccaa ttttatttct atcaatataa ttagtgtggc tgtaacaaca atcttcggtc
     5281 atagcatatg cctgctgaat ttttatgccg attttccaaa accagttaat tgtatccacc
     5341 gaaattggtg ctgcaccgga aaaacattgt tctgcttttg acaagcccaa cgctgcttta
     5401 atttttttct tgactatgcc attaacaata ggaattttca acaaacgatt gagtttttgc
     5461 tgcggcattt tttccaatat tttttcctgg aattttgccc agattctcgg tacggccaaa
     5521 aacaaggtgg gcgaagtttc ctgcaaattt ttaggaaatg tttccagact ttctgcaaat
     5581 gaaacctgac cgccggtgta taaactgccc atttcaatta ataatctttc cgcaatgtgg
     5641 cacatcggta aataggagaa gaaaactgca gtattatcaa cttttaattc cggaaacgca
     5701 tattctgctg caaatccaaa attgtaaaaa gtatgcataa cacctttagg cacacctgtt
     5761 gttccactcg tgtacatgat ggtgcacaag ttttgcatag gataatcggg attttcttta
     5821 atcggagcat tattttttat gatatcatcc cagtttttat attcagtaat gccataaaat
     5881 ggataagcaa cgcaaggcaa attttctggt acaccgggtt tcaaataagc ccaatcgtct
     5941 aattttccga caaacaacat tttgctttcg ctgtgttcga gaatttgtct aacagtattt
     6001 ggcgacatat tcggatacat tggaaccgat acataacctg ccaaccaaat ggcaaagtcg
     6061 ctcagcatcc aatgcacaca gtttttcgac aggatagcaa tgcgactgcc cggctcataa
     6121 ttgagtgatt tcaaataagc tgccattctg cgaacctgat caccacattc gccccaggta
     6181 taggtttgcc attcgccatt tattggttgg cggaggtata atctgttgcg ttgtgcattt
     6241 tcccatttgt aaaacgttgc caaagggctg tgaactgttc tttctatcgc cataatactt
     6301 actttttaac cgttgtcaat acaattatag tcactaagat aataacctgt gacagatatt
     6361 gtaaaatctt tatgcaattt tgtggttcca aagggcaaaa gccaataatt atgagcgaac
     6421 aagtaaacag aaccgaaatt tcatcactgg gtgaatttaa actgattgaa caactgacta
     6481 aaaactttga attgcgcaat gcatcaagtt taaaagggat tggcgatgat gctgcagtga
     6541 ttgacaatac caataaatta actgttgtaa ctacagatat gctggtagaa ggcattcatt
     6601 ttgatatgat gtattctcca cttttgcatt taggatataa atgtattgca gtaaatgtga
     6661 gcgatttatt ggcaatgaat gctgagccaa aacaagttac agtttccatt gctatttcga
     6721 acagatattc agtggaagct atggaagaat tatacgaagg tatttaccat gcttgcgaat
     6781 attatggtgt tgatttaatt ggtggagata caacatccag tttaaaagga ttaattattt
     6841 ctgtaactgc aattggagaa gccaatgaag atgaaattgt atatcgcagc ggcgcaaaag
     6901 atggtgattt aatttgtgta agcggagatt taggtggtgc gtttttagga ttacaattat
     6961 tagaaagaga gaaaaaaata tttttagaaa atccgggcat acaacctgat ttagaaaata
     7021 aaaaacatat tgtcggtcga tttttaaaac cggaagccag gaaagatatt gtagaagcga
     7081 tgagagctca gtcaatagta cctactgcaa tgattgatat ttctgatgga ttatcgtctg
     7141 agatattaca tatctgcaat caaagcaatt gcggtgccat tatttatgaa aatgaaattc
     7201 ctattcaccc tgaatcttat gaccaggcgt taatatttaa tatggaccca acagtatgcg
     7261 cattaaatgg tggtgaggat tacgaattat tatttaccat tgacccaaaa catgcgactg
     7321 ctattgcaga aatcccgaat gtttccgtaa ttggtaaaat tgtaccgcaa gctaatggta
     7381 catttctgaa aacaaaaaat gataatttat ttaaaataca agcccaggga tgggaaagtt
     7441 ttaagcagga ataattaatc gtcgagataa aataatttat tatatacttt tccttcaaca
     7501 aataccgaca acacgtaaat tccgcgtgcg tgttgttcaa tatttatgct gaatattctg
     7561 cctttttcaa ctttataaaa agcgccttca actattgttt gtcctaaagc attacatact
     7621 aaaatatcag ctttgtcttg ggtatcgata ttaatgacaa acttaattac ccctttaacc
     7681 ggattttgtg taatccataa aatttcatct tccggaatta taagcgaatc aactccggtt
     7741 gtatcttcag cttcaatttc tgtaaaacaa tcgtagggta atccaaaaaa ccacggtgta
     7801 gaagttggtt cagtgctgaa aaaagtaaat aagcgatctg ccgctttttg ttgacctgac
     7861 ccggataaca aaaacccatc ctgatcggta tcaaaatcgc cggggcataa ccaacgtaaa
     7921 tcatcatatt cacgtaatgt tcttccatca gcccagaaat tcggtcccca tgcaacccag
     7981 gctgcaggag cgtcttcgcc ggaatatttt aacaaagtat cgccttcaat ttgttgttga
     8041 attattgctt ttatggcaaa gtcgttaaaa taaggtgccg gttcaccaac agcagtatag
     8101 tgctcgccga gtgtatcact atatccccca taatgtaaac cggaaatata aatgagtttt
     8161 aaattaatga actggtcttt taatttgcga attacttcgc gataggtatt ggtgagtgta
     8221 tcaacgtatt gttctgccgt aaaaaaagta tcttcaaaag aaatggcctg catccaaaca
     8281 acactcactt gttttttctt taaatcaacg ttctgaaaaa aatcatttac atctttccaa
     8341 tacacatcat ttgcatcaga tgaaatcata tcatgtaaac tataattttc catacatgca
     8401 ttgattaatc gcacacatga atcggtataa ccctgattaa gatattcgcc aatgaattta
     8461 ttaaatgatt tcccggctga gccatttcct aaacccacca ttaccacttt gccataggta
     8521 gtgtctattt caccatcaaa attaattggc ttaatggcat tagcgtgtac tacacctgag
     8581 tcggcatgag cagcaggcat aacattcaaa ccgccgggat acaatccgcc ctgaaaacct
     8641 aaatacgtat caaattgtaa atcgacaata ggtatgagca aagaactatc attatcacaa
     8701 ttgatttgcg catcaagagt aaaatgaaag aggataagtg tgattgcact tatcctcctc
     8761 aacatgatat ttagtaatac aggcatttac ctgttaataa ttataaatta atttaaaaca
     8821 ataggttgag aaaataattg attgccaatt accacctcaa aataataaat tcctgcaggt
     8881 tttcctgaca aatcaaattg gtaaaacgaa aacggctgaa cagaaccata agattcgctc
     8941 attacaactt ctcctatttg attaaccact ttaacctgaa tggcttcagt taacacttca
     9001 tcgaagtcaa cataaaactg accgtttgaa gggttaggga aaacagtaat atcttctttt
     9061 gtaataattg tttcttcagg taaaataata ctaccatctg ctaaacgacc atcgccacag
     9121 ctagcccatt taggaccatc cataaaccaa ctttcgctta atgtatctgt tctgaaaaag
     9181 tcgagtaaaa tatttgccaa tttgtctttc ccttcattgg tcaaatggta tccaccacca
     9241 tcaattgcaa attcagcttc acaattccag gtaagtccgt cataatcgcg aggatttttt
     9301 ccatcagccc ataacggtgg tccccaaatt
//
LOCUS       DAORKW010000081         9239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_189_length_9239_cov_4.165941, whole genome shotgun sequence.
ACCESSION   DAORKW010000081 DAORKW010000000
VERSION     DAORKW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189_length_9239_cov_4.165941"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLB46_04935"
     CDS             complement(<1..661)
                     /locus_tag="PLB46_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF75895.1"
                     /translation="MHTTISNSARTQSYIDKEEKFGAHNYHPIPVVIESAEGVYMWDV
                     DGKRYYDFLSAYSAVNQGHCHPKIISALIHQAEKLTLTSRAFHNDLLGQYEEYMSKLF
                     GYDKLLPMNSGAEAVETAIKLCRRWAYDVKGVPANMAKILVCDGNFHGRTTGIISFST
                     DPDSTSGFGPFIPGYEVVAYNDLTSLANALKDPNVAGFLVEPIQGEAGVYVPDEGFLS
                     SA"
     gene            959..1342
                     /locus_tag="PLB46_04940"
     CDS             959..1342
                     /locus_tag="PLB46_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRF75896.1"
                     /translation="MKKSHIIVLFLIAICIGVFASKLGNVSSYSSYNDAKSKEGKTVQ
                     LIGTLTPGKAVNFDPIKDPNSFSFYLNDRDGNEVHVVCFDDMPTDFEKSEEIVLTGSM
                     KENTFYADEMLVKCPSKYMENEIQK"
     gene            1355..3856
                     /gene="ccsA"
                     /locus_tag="PLB46_04945"
     CDS             1355..3856
                     /gene="ccsA"
                     /locus_tag="PLB46_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRF75897.1"
                     /translation="MEELSHQHAVQAINPLFGQIGHFFVITAFICSLFAAIYYFRSTR
                     NQQKIIQDNAHKIARLFFIGHIISILAVVITLFIMIFNHHFEYYYVWSHSSTDLQMKY
                     VLSCFWEGQEGSFLLWSFWHVVLGIVIMLKAGKWESPVMSIVSLMQVFLLSFILGIYI
                     TDTLHIGSSPFLLLRDKLTGDPVFLFDDYMRFIPDGRGLNALLQNYWMVIHPPVLFLG
                     FASITIPFAYAIAGLWNKDYTGWIKPAIPYALFASMVLGTGILMGGAWAYEALSFGGF
                     WAWDPVENASLIPWLIIVAGLHNLVISKATGHALRSSFVMIILAFFFVVYASFLTRSG
                     ILGDTSVHAFVEEGLTNHLLIFLFTFVIISKYFLIVNWKKIPRKEKEEALSSREFWMF
                     VGSLVLLFGGLHIIFFTSIPVFNTIIGHINDLFGSSIRDDMGKPDNEIQFYSGVQIWV
                     GVLIAILSAFGQYLKYKATESRVFFRSIMANFIFAIIATVISSILLDKLWSITADRGI
                     FHLITSYSILFFAGWFSVIANMQYLFSVLKGKIKIAGGAISHIGFGVLILGILISNAG
                     QRVISENNFGVQFDGEGMDKTFQRENVRLIKNAPLPLGDYLVTYLGDTIEQGATYYEL
                     NFRKIDPETGKIQQDFNVRPYLLMDTKMQQLAPNPDTKHYLTYDVFTHVTSLPGEGSK
                     NAPPTVKTDTIGMGDTLRFNTGYLLLNDVVRFTSNADSIGVMAKFDAVIGVAKEELTP
                     RFVIRLADGTAQQGTAQATIGNVQVTFLGILPEQNKFVFTVTDATLNTDWIVIKSIIF
                     PMINLVWLGTIVMAIGFFISMRKRIQDNKVSAKNA"
     gene            3858..4643
                     /locus_tag="PLB46_04950"
     CDS             3858..4643
                     /locus_tag="PLB46_04950"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HRF75898.1"
                     /translation="MQIQAITIIGTGNVATHLGKAFLKAGIQINAIVARDQQKAIALG
                     ALLNNAPGLLLGDILPPSDAYIIAVKDDAIHEVAKAVAHKDRLLIHCAGASNGNILDI
                     KGNEFGVMWPMQTLTKNNDVALHQTLIAVSGSNDATLMAIAALARKISERIIEVSEPH
                     RAMLHLSPVWINNYTNHMFVIAEQLLKENNLDFNLFIPLIDEHINKLKTMSADLLQTG
                     PASRGDMVTLNKHRSMLKAHPEILQLYDVLAQSVLNHSLKNKS"
     gene            4652..4924
                     /locus_tag="PLB46_04955"
     CDS             4652..4924
                     /locus_tag="PLB46_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRF75899.1"
                     /translation="MITIKYNFEDGSEPREIQIEPGYTVLEAALLNDIKLNHNCGGVC
                     ACSTCHVYIEKGMEHLPETTEKEEDFIDRARDPRLNSRLSCQCDIM"
     gene            5002..5244
                     /gene="iscX"
                     /locus_tag="PLB46_04960"
     CDS             5002..5244
                     /gene="iscX"
                     /locus_tag="PLB46_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HRF75900.1"
                     /translation="MNHFEEPPIYWADHEDIALKLYERFGDDFNESKIYRVRFTELIE
                     WVLEVPEFKGTREECNEGHLEMIQSAWVYEWRDAQK"
     gene            5360..6292
                     /locus_tag="PLB46_04965"
     CDS             5360..6292
                     /locus_tag="PLB46_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HRF75901.1"
                     /translation="MKAWFQLIRPINLVLLMLTQYCIDWFILQPNFTTYGLHFTLNEW
                     QFFLLVLSTTLICAGGYIINDYFDVDIDNINKPGKQIIGKTIAPQRAFYGYLLLTGIG
                     LGLGIYLCLAIDYWKLITVFVVVIFLLYFYSATFKKTAFIGNLIVAVLAAMSVIIVML
                     FEPSLYQLARPGDYYIAGICIDYITGISFFAFALTMVREIVKDIQDMDGDRRHQAKTL
                     PIKFGVKTAKAIAIGFVLITMAALGYIYLDVLQIFGSLYLIYIVILMVFLAVIAVRLF
                     TSATKNEYGLVSTLLKAAMFLGLGLMPFYYLLEF"
     gene            6295..6861
                     /locus_tag="PLB46_04970"
     CDS             6295..6861
                     /locus_tag="PLB46_04970"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRF75902.1"
                     /translation="MSIVLASGSPRRSQLLADAGFTFTVIKPEVDESFDAAIPVMDVP
                     LIIANNKMAVAKTMCNANDIIITADTVVVLEDTIIGKPTDRDDAIRILKALSGKMHTV
                     VSGVCISQGTRQYDITVSTKVYFDVLQEAEILYYLDKYQPYDKAGAYAIQEWIGINKI
                     TRIEGDYYNVVGFPMSKIFPVLSALLEQ"
     gene            complement(6973..7818)
                     /locus_tag="PLB46_04975"
     CDS             complement(6973..7818)
                     /locus_tag="PLB46_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75903.1"
                     /translation="MQADDFIKLVDNPNFISGIYNYCNRWCDKCPFVAQCTVGTMEQL
                     IHFGRTSNEPITLDEFTGMMEIATGTSINKEDVKTITINVEELMELDEDEPSLLNDVS
                     TSDPDSDFEAEFKAAFEFVESHDIIALAHPYSISCYKWKNHMSKYFYFDESSENHSCQ
                     YIGKFPIKSQQQKAELIEAFETVVWYVLQIEIKLKRALHGFFTDKDDYLPGDYQTDFN
                     GSAKIAVIGINSSLQAFEKLYAFFTEEQSSIKNKMGLLESVRAKTYEQFPNLDAFIRP
                     GFDKE"
     gene            complement(7824..9002)
                     /locus_tag="PLB46_04980"
     CDS             complement(7824..9002)
                     /locus_tag="PLB46_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF75904.1"
                     /translation="MDISAIRNDTPGTRLIKHFNNAGCSLHPKPVVDAVQQFLEEESL
                     IGGYEIAAKREAEIAEFYAVAATMLNCKSTNIAFVSSATDGFAKALSSVPFKQGDYIL
                     TTINDYASYQIQFLSLQKRFGIQIIRAANTPSGEVDINSMHELMKKYKPVLVSVTHVP
                     NNTGMIQPVALIGDLCEQENILYAVDVCQSAGQMPLDMQKIKCDFASFTLRKFMRGPR
                     GAAVLYVSDKVLNLGLEPLFIDMRGADWIQQNSYSPIPNATRFEFVEQSYAILIGAKV
                     AIQYYLNLGQENVFARNRVISAYTREKLAAIPGITLMDQGNHLSNIISALPAKKNIFE
                     IKNELNRLGINIGAAQKKFALLDFEQKHMEGTLRISPHYFNTHEEIDILVDSIHCLNP
                     "
     gene            complement(9012..>9239)
                     /locus_tag="PLB46_04985"
     CDS             complement(9012..>9239)
                     /locus_tag="PLB46_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRF75905.1"
                     /translation="IPALVNQIQKRGREYEDNLRLDYLRRLNEYYEAWIAKYKHGKLL
                     IIDIDHCNFAENAEDLGSVIQRVNTELYGLF"
BASE COUNT         2864 a         1559 c         1801 g         3015 t
ORIGIN      
        1 aagcagaaga taaaaatccc tcatccggga cgtagacccc cgcttcgccc tgaataggtt
       61 ctaccaggaa accggctaca ttagggtctt tgagtgcatt tgccagtgaa gtgaggtcgt
      121 tataagcaac aacttcatag cccggaataa aaggaccaaa tccggaggtt gagtctggat
      181 cggtgctgaa agagatgata ccagtggttc tgccatgaaa attgccatca caaactaaaa
      241 ttttcgccat attagcaggc acacccttaa catcataagc ccaacggcga caaagtttga
      301 tagctgtttc taccgcttct gcaccggagt tcataggcag taatttatca taaccgaaca
      361 atttgctcat atattcctca tattggccca ataagtcgtt gtgaaacgcc ctgctggtga
      421 gggttagctt ttctgcctgg tgaatgagtg cactgataat tttggggtga caatgtccct
      481 gatttacagc actataagct gaaaggaagt cgtagtaacg ttttccgtcc acatcccaca
      541 tataaacacc ttcggcactt tcaattacaa ccggtatcgg atggtaatta tgtgcgccaa
      601 atttttcttc tttatcaata taggattgtg ttctggcaga atttgaaata gttgtatgca
      661 taaaatgatg tattttaacg tatagaattg gttgaaaaaa taatgtgtaa aaataaagcg
      721 ggtaaacaac aaaatgcggc ggtaatatgc cttattaccc gggattgatt aatccaagtg
      781 gtggtcgagg aagtcgagat tcatatctaa aaaatgtata tccacgatgc aaagataagg
      841 ttaatttgac aatcggtttc aacaatggcg gcaatttgtg caaaatggtt gaaaaacaag
      901 gctgtaaaac gaacattttg acctaatttt gcattatcaa ttaaaccaaa gatatacagt
      961 gaaaaaatcg catatcatcg tcctcttcct cattgcaata tgtatcggcg tttttgcctc
     1021 aaaattgggt aatgtgagtt cttactcaag ttacaacgat gctaaaagca aagagggtaa
     1081 aacagttcaa cttattggaa cacttacacc gggtaaagca gtcaactttg acccaattaa
     1141 agacccgaat tcgttttctt tttatttaaa cgaccgcgat ggaaatgaag tgcatgttgt
     1201 gtgtttcgac gatatgccaa ccgactttga aaaaagtgaa gaaattgtgc ttaccggctc
     1261 aatgaaagaa aacacttttt atgcggatga aatgctcgta aaatgcccgt ccaaatacat
     1321 ggaaaacgaa attcaaaaat aaggggcact atacatggaa gaactttctc atcagcacgc
     1381 agtgcaagct ataaatccgt tgttcggaca aataggacat ttttttgtga taacggcttt
     1441 tatttgttcg ttatttgcag ctatttatta tttccgctca acacgcaatc aacaaaaaat
     1501 tattcaggat aatgcccaca aaatagccag actatttttt attggccata tcatttccat
     1561 ccttgccgta gtaattacat tattcattat gatatttaat catcattttg aatactatta
     1621 cgtttggtca cattcttcca ccgacttgca aatgaaatat gtattgtcct gtttttggga
     1681 aggacaggag ggaagctttc tgctgtggag tttttggcat gtggtgttgg gtatcgtaat
     1741 aatgctgaaa gccggcaaat gggaaagtcc cgttatgagt atagtgagtt taatgcaggt
     1801 atttttactc agctttattt taggtattta tattactgat actttacata ttggcagttc
     1861 accatttttg ttattaagag ataaactaac cggtgaccct gtatttttat ttgatgatta
     1921 tatgcgtttt attcctgatg gacgaggttt aaatgcatta cttcaaaatt attggatggt
     1981 aatacatccg cctgttttgt ttttaggatt tgcatctatc actattccat ttgcttacgc
     2041 gattgcaggt ttgtggaata aagattatac aggatggata aaaccggcta ttccttatgc
     2101 attgtttgca tcaatggtat taggaacagg tattctaatg ggtggtgcct gggcatatga
     2161 agcattaagt tttggtggat tttgggcatg ggacccggtt gaaaatgcat cacttattcc
     2221 gtggttaatt attgtagcag ggttacataa tctggtaata tcaaaagcaa ccggacatgc
     2281 attacgctcg agttttgtga tgattattct tgcattcttt tttgtggtgt atgcatcttt
     2341 tctcacacgc agtggtattt taggtgatac cagtgtacac gcatttgtgg aagaaggact
     2401 tactaatcac ctcctgatat ttttattcac gttcgtaatt atttcgaaat actttttaat
     2461 tgtcaactgg aaaaaaattc cgcgtaagga aaaagaagaa gcactttctt cacgtgagtt
     2521 ttggatgttt gtagggtcat tagtgttatt gttcggcgga ttacatatca tatttttcac
     2581 ttctattccg gtgtttaata ccatcatcgg tcatatcaac gatttgtttg gttcatctat
     2641 tagagatgat atgggaaaac cggataatga aattcagttt tattccggtg ttcaaatttg
     2701 ggtaggtgtg ttaattgcca ttttatctgc atttggtcaa tatttaaaat acaaagccac
     2761 tgaaagtagg gtatttttcc gcagtataat ggcgaatttc atttttgcta ttatcgctac
     2821 tgttatcagt tcaatattac tggataaatt gtggagcatc actgcagaca gaggtatatt
     2881 tcatctcata acatcttatt ctatcttatt ttttgcgggt tggttcagtg tgattgccaa
     2941 tatgcaatat ttgtttagcg tgttaaaagg gaaaattaaa attgcaggtg gtgctatatc
     3001 acatattggt ttcggtgttt taatacttgg tattttaatc tccaatgcag gacaaagagt
     3061 aatttctgaa aataatttcg gagtgcaatt tgatggcgaa ggaatggata aaacattcca
     3121 gcgcgaaaat gtgcgcctta ttaaaaatgc accattgcct ttgggtgatt atttagtcac
     3181 ttatttggga gataccattg aacagggtgc gacttattat gaattaaatt tcagaaaaat
     3241 tgatccggaa acaggaaaaa tacaacaaga ctttaatgtg cgcccttatt tgctgatgga
     3301 tactaaaatg cagcaattag ctccaaatcc ggataccaaa cattatttaa cttacgatgt
     3361 atttacgcat gttacatcat tgccgggtga aggttctaaa aatgcacctc caacagttaa
     3421 aacagatacc ataggaatgg gcgatacact cagatttaat acaggttatt tattattaaa
     3481 tgatgtggta cggtttacaa gtaacgcgga tagtattggt gtaatggcaa aatttgatgc
     3541 agtaataggt gtggcaaaag aagaattaac accaaggttt gttattcgcc ttgcggatgg
     3601 tacggcacaa caaggaacag cacaagcaac aattgggaat gttcaggtta cctttttagg
     3661 cattttacct gagcaaaata aatttgtatt taccgttacc gatgctacat taaataccga
     3721 ttggattgtc attaaatcta tcatattccc tatgataaat ttggtatggc tcggaactat
     3781 tgtaatggct atcggatttt ttatcagcat gcgcaaacgc atccaggata acaaggttag
     3841 tgctaaaaac gcttaatatg caaattcagg caattacgat tatcggaaca ggtaatgtgg
     3901 ccacacattt gggcaaagct tttttaaaag ccggtattca aattaatgcc attgtggctc
     3961 gtgatcaaca aaaagcaata gcattaggag ccttgttaaa taatgcgccc ggtttattgt
     4021 taggagatat attaccgcca tctgatgcct atattattgc tgttaaagat gatgctattc
     4081 acgaagtagc aaaagcagtt gcgcataaag accgattatt aattcattgt gcgggtgcat
     4141 ccaatggcaa tatattagat ataaagggta atgagttcgg tgtaatgtgg cctatgcaaa
     4201 cgcttactaa aaacaatgat gttgcattac atcaaacatt aattgccgtt tctggaagca
     4261 acgatgctac acttatggct attgctgctt tagctcgaaa aatatctgaa cgaattatag
     4321 aagtatcaga accacacaga gcgatgttgc acttaagtcc agtatggata aataattata
     4381 ccaatcacat gtttgtaatt gcggaacagt tactcaaaga aaacaactta gattttaatt
     4441 tatttattcc gctcattgat gaacatatca ataaacttaa aactatgtct gccgatttat
     4501 tgcaaacagg tccggcatca agaggcgata tggttacact taacaaacac cgaagtatgc
     4561 tgaaagcaca tcctgaaatt ctgcaattat atgatgtgct ggcgcaaagt gtgttaaatc
     4621 attcattaaa aaacaaatcg taattcaggc tatgataact attaaatata attttgagga
     4681 cgggagcgaa ccacgggaaa ttcagattga accggggtac actgttttag aagcagcatt
     4741 attaaacgat attaaactca atcataattg cggcggtgtt tgtgcatgca gcacctgtca
     4801 tgtgtatata gaaaaaggta tggaacatct tcctgaaaca acagaaaagg aagaagattt
     4861 tatcgaccgt gcacgcgatc cacgcttaaa ttcccgattg agttgtcagt gtgatatcat
     4921 gtaagacaat acttatatcg aggtaattgt tccagatcag tctactatta ttgcatcata
     4981 aaaatatata tccaaaatac tatgaaccat tttgaagagc caccaattta ttgggcagac
     5041 catgaagata tcgcattgaa attgtacgaa cgatttggtg atgattttaa tgaatcaaaa
     5101 atttatcgcg ttcggtttac cgaattgata gaatgggttt tggaagttcc ggaatttaaa
     5161 ggaacaagag aagaatgtaa cgaaggtcat ttagaaatga tacaaagcgc atgggtttat
     5221 gaatggcgcg atgcacaaaa ataaattacc tacaattgca tacattttat atacaaatta
     5281 tctgtaattg tagataatcg gttatacggt atactagttg gtaacactca atcgttattc
     5341 aatcaacaaa caacacactg tgaaagcctg gtttcaactt attcggccca ttaacctggt
     5401 attgttaatg ctcactcaat attgcattga ctggtttatt ttacaaccta attttaccac
     5461 gtacggatta cattttacgc ttaatgaatg gcagttcttt ttactcgtgt tatctactac
     5521 tttgatttgt gccggtgggt atattatcaa cgattatttt gatgttgaca tagataacat
     5581 taataaaccc ggcaaacaaa taatcgggaa aacgattgct cctcagcgtg cattttatgg
     5641 ctatttgttg cttaccggaa ttgggttggg attgggcatt tatttatgtt tagccatcga
     5701 ttactggaaa ctaattacgg tttttgtagt ggtgatattt ttattgtatt tctattccgc
     5761 aacatttaag aaaacggcat ttattggcaa tttaatagtt gctgttttgg ctgcaatgag
     5821 tgttattatt gtcatgttat ttgagccttc gttatatcaa ttggcaagac cgggtgatta
     5881 ttatattgcc ggaatttgta tcgattatat taccggaatt agtttttttg cttttgcctt
     5941 aaccatggtg cgggaaattg ttaaagatat tcaggatatg gatggcgaca gaaggcatca
     6001 ggcaaaaacg ctgcctatta aatttggtgt aaaaactgca aaggctattg ccataggttt
     6061 tgtgctgata accatggctg cattgggtta tatttattta gatgtattgc agattttcgg
     6121 atctttgtat ttaatatata tcgttatttt aatggtattt ttagcagtaa ttgctgttcg
     6181 gttatttaca tctgcaacta aaaacgaata tgggttggta agcactttat taaaagctgc
     6241 catgttttta ggtttaggat taatgccatt ttattatcta ttagaatttt aattatgtcg
     6301 atagttttag cttcaggatc accacgccgc agtcaattat tggcagatgc cggatttaca
     6361 tttacggtaa ttaaacctga ggtagatgag tcgtttgatg cagcaattcc tgttatggac
     6421 gtgccgctga taattgccaa taataaaatg gctgtcgcaa aaaccatgtg taatgctaac
     6481 gatattatta taactgcaga taccgttgtt gtgttagaag ataccattat tggtaaacca
     6541 acggacaggg atgatgcaat acgcatatta aaagctttgt caggtaaaat gcatactgtt
     6601 gtttccggtg tatgtatttc tcaagggaca cgacaatatg atattaccgt aagtaccaaa
     6661 gtgtattttg atgtattaca ggaagcggaa atactttatt atttagataa ataccagcct
     6721 tacgacaaag ccggtgcata tgccatacag gaatggatag gcattaataa aatcaccaga
     6781 attgagggcg attattataa tgtggttggt ttccctatga gtaaaatatt tcctgtgtta
     6841 tctgcgctgc tagaacagta attctatcgc attattaata aaattacttg ctgataatcc
     6901 ggaacaccgg tatcagtctt cgattgaaat taactgatac cggtataatt tcggatattg
     6961 ttctatcatt aattattcct tgtcaaaacc aggacgtata aatgcatcca ggttggggaa
     7021 ttgttcatag gttttggcgc gaaccgattc caataatccc attttatttt taatagagga
     7081 ctgttcttct gtaaaaaaag catacaattt ttcaaatgcc tgtaaagaac tattgatacc
     7141 aattactgca atttttgccg aaccattaaa atcggtctga taatcaccgg gcagataatc
     7201 atctttatca gtaaagaaac catgtaatgc tcgtttaagt ttaatttcaa tttgtaatac
     7261 ataccacacc acagtttcaa aggcttcgat taattccgct ttttgctgtt gtgatttaat
     7321 aggaaattta ccgatatact gacagctgtg gttttcggaa gattcatcaa aataaaaata
     7381 tttactcatg tgatttttcc atttataaca actaatggaa taggggtgag cgagtgcaat
     7441 aatatcatgc gattcgacaa attcgaaggc agctttaaat tccgcttcaa aatctgagtc
     7501 ggggtcgctt gttgatacat cgttgagtaa acttggctca tcctcatcaa gttccatgag
     7561 ttcttctaca ttaatggtaa ttgttttaac atcttcttta ttaatagatg tgcctgttgc
     7621 aatttccatc ataccagtaa attcatctaa tgtaatcggt tcattggaag ttctgccaaa
     7681 atgaattaat tgttccatag tgcctacagt acattgcgca acaaaaggac atttgtcgca
     7741 ccaacgatta caataattgt aaataccgga aatgaaattg gggttgtcta cgagtttaat
     7801 aaaatcatct gcttgcatat taattaaggg tttaaacaat gaattgaatc cacaagtata
     7861 tcaatttctt cgtgagtatt gaaataatgt ggactaattc tgagtgtccc ttccatatgc
     7921 ttttgttcaa aatctaataa ggcaaacttc ttctgagcag caccaatatt gatacctaaa
     7981 cggtttaact cgtttttaat ttcgaatata tttttttttg caggaagtgc agaaataata
     8041 ttacttaagt gatttccctg gtccattaaa gtaattccgg gaattgcagc taatttttcc
     8101 cgtgtataag cgcttatgac acggtttctt gcaaacacat tttcttgtcc caggttgaga
     8161 taatattgta ttgccacttt tgctccgatg agtatggcat aagattgttc cacaaattcg
     8221 aaacgagtgg cattgggtat aggactataa ctattttgct gaatccaatc cgccccacgc
     8281 atgtcaataa acaacggttc taatcctaaa ttcaaaactt tatccgacac atataaaaca
     8341 gcagcacctc ttggtccacg cataaattta cgcagggtaa atgaagcaaa atcgcatttt
     8401 attttttgca tgtccaaagg catttgtcct gcactttgac aaacatcaac tgcgtataat
     8461 atattttcct gttcacataa atcgccaatt aaagctaccg gttgaatcat acccgtatta
     8521 ttaggaacat gggttacgga aactagaaca ggtttatatt ttttcatgag ttcgtgcatg
     8581 gaattaatat ccacttcacc tgaaggggtg tttgctgcac gaattatttg tataccaaat
     8641 cttttttgta aagaaagaaa ttggatttga taagatgcgt aatcgttgat ggtggttaat
     8701 atgtaatcac cttgtttaaa tggcacagat gataatgctt ttgcaaaccc atcagtagca
     8761 ctgctaacaa aggcaatatt ggtacttttg caattgagca tggtggcagc aacggcataa
     8821 aattccgcaa tttcggcttc tcgtttagct gcaatttcgt aaccaccaat tagtgattct
     8881 tcttctaaaa attgctggac agcatcaaca acaggtttag gatgcaaaga gcagccggcg
     8941 ttattaaaat gtttaattaa acgtgtcccg ggagtatcgt tgcgaattgc cgaaatatcc
     9001 ataaagggaa attaaaacaa tccgtataat tcggtgttaa cgcgttgaat tacactacca
     9061 aggtcttctg cgttttctgc aaaattgcag tggtcgatat cgataattaa taatttaccg
     9121 tgtttgtatt tggcaatcca tgcttcgtaa tattcgttta agcgacgcag ataatcgaga
     9181 cgtaaattgt cttcatattc gcgaccgcgt ttttgaattt gattaactaa agcaggtat
//
LOCUS       DAORKW010000082         8999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_195_length_8999_cov_5.643336, whole genome shotgun sequence.
ACCESSION   DAORKW010000082 DAORKW010000000
VERSION     DAORKW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195_length_8999_cov_5.643336"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLB46_04990"
     CDS             complement(<1..276)
                     /locus_tag="PLB46_04990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRF75906.1"
                     /translation="MYFVKTPWYLKTVYPSLVWDIPKSKTVYLTFDDGPVPGVTDKVL
                     DILSDYHIKATFFCIGDNVVKHHNLFLRQAHGEGAALAGGALPGDTAG"
     gene            991..1236
                     /locus_tag="PLB46_04995"
     CDS             991..1236
                     /locus_tag="PLB46_04995"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75907.1"
                     /translation="METEKKIEIFVNLIAKIESITNFTEQEIETILKAFMEDNYQGMG
                     KRRPILRAALVDSLQRPPVFAMVDVLRKNNSVARLHN"
     gene            1281..1793
                     /locus_tag="PLB46_05000"
     CDS             1281..1793
                     /locus_tag="PLB46_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75908.1"
                     /translation="MPKKKPLEGKPEVHKDLDGFEMNINEFGEISSNTDIDKLNKFLN
                     KNVDDKKFKGLEDLPYAKLGDDDDDDAVKDEEDDLNEIADEFEEFRQFNDKDFKDEFD
                     FDEKDEKEDFGFDEGKHMYEDLDGDDDKKIDDDDDDDFDEEEFVDDMADEFGEEDNYN
                     FDDGDFDDRY"
     gene            complement(1855..2565)
                     /locus_tag="PLB46_05005"
     CDS             complement(1855..2565)
                     /locus_tag="PLB46_05005"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRF75909.1"
                     /translation="MSAFHFKQFSIQQGETAMKVGTDGVLLGAWVDARLIGEKVLDIG
                     TGTGIIAIMLAQKKPDARIDAIERDENAFQQAVGNIALCPWYNQIKVYPIDLQQYLLS
                     CTTTYATIVCNPPYFIKGWQIDNPGRKLARDAEHLPHDLLIAAAKQLLTVDGNLWIVL
                     PKQEGELFITAALQEQLYCCKKTQVFTKPGIVAKRLLLRFSKQKMEVVENELLIADEQ
                     NNYTDAYKALTKDFYLNF"
     gene            2641..4224
                     /locus_tag="PLB46_05010"
     CDS             2641..4224
                     /locus_tag="PLB46_05010"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRF75910.1"
                     /translation="MKKNLLLTFVSIAALSNINAQLIVTEEPNDTTFIYSLAGPGIVV
                     SGIERNCAEGASGFFDATSANVGIDNGIVLTSGTLLNVVGPNNLSSATGYNGTAGDDD
                     LTPLTIWETYDACAISFDMNVAADTLKLQFVFGSEEYAEFVGSGFNDVFAFWVSGPGM
                     PDPVNIAVVPGTETPVTINNINIDTNPDYYIYNGDGYSDPYASDPAYVQYDGLTTVML
                     ATIPVTAGETYHMKMIVADAGDAILDSGVFLETGSLGSLRMQYQTLADNDLTNAVEKC
                     AKGYFKLINEVPCADPLVIDYYIAGSATNGVDYEMLPGQITIPAFVSEGIIEVNPIPD
                     AVDESLESVVLYLFNPQSGFVYDTLTLLIDDEAAPASFTAENDDLVYTFTETSGIASS
                     VTWEFGDGITSTEMNPTHTYLTEGVYTVCVTSINIYGCSDTYCEEVMVGAVGINNLSN
                     DLTISPNPANNYITVNYSGVISSDLTITIKNVTGTIVSTTTSNAPTTTLDVQSLASGI
                     YFIELNNGGVISVQKIEKL"
     gene            complement(4285..6888)
                     /locus_tag="PLB46_05015"
     CDS             complement(4285..6888)
                     /locus_tag="PLB46_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75911.1"
                     /translation="MKKKIALLIVAGTILLTFIVMAVVFQKQKDGNNFHQSEPPQPTR
                     VMEHRNDEDKNKEEREAFVNLMHNAAPGTNWMKMDADLRYKKMLERANYYKYRTTGGE
                     WDTLAEGNVIGKWDEIGSFNTSGRIWATDIDFENDNIYAFTDGGNLWKGDLNGDNWSV
                     INDNFKLTGTHMLRKMDDRLIAASSAWGTQGVFYTEDEGLSWTSATGMENVATWGYVF
                     DAEILNNAEHTMLVLAYEWDYTNWYDIVSIYKSDNLGESFEKITSYDVPTYGGPNKFV
                     LWSAVNGDPISYFIHNDTVITIDATGVLTTTGILPITDDGDIMLCGFTGLTNTTLYAA
                     HNNYALGKTKFYKSVDAGVNWSATGEVDEGNFSKNSFNCSQKTEGYLYYGGVDSYRSF
                     TGGDTWVRNNYWWEYYDDVEYNLHADIPYIRTYNNPAGGDEIVLVSTDGGLFKSTNNG
                     LIWNNITNEGMRNAQYYDVYTYRFVPDIIFAGAQDQGYQRSTYEVDNDYYFDQLISGD
                     YGHIVSRSGGDNLWCVYPGFAMYINDAASGSDMFFWDFVGTGHLWMAPIMADPYEDEV
                     AWWGGGSEGGGAYLWRLEKSGADITGVKQIKNFAVAGGGSISAINYSPVNPEYWYLLT
                     SGGNFYYSENGGDTWTMTSSFSGPGSHYFYGATIEPSKTDADVVYIGGSGYDNPAVYV
                     SSNHGESFTALTDGMPSTLVYDLAISTDDSLLFAATEVAPYVYVKAENKWYDLAGFDA
                     PLQTYWSVDFVDEIQAARFGTYGRGTWEFKLEDETPNIAVNNLNENGVLQLYPNPASD
                     VVRLKLNMFLPDATIEIINMQGKTVLSKNAAINKDVAFLINISTLPAGNYFLRIKGNG
                     NQFVEKLVVTK"
     gene            complement(6912..8531)
                     /locus_tag="PLB46_05020"
     CDS             complement(6912..8531)
                     /locus_tag="PLB46_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRF75912.1"
                     /translation="MMKNIVTPLLLMLLSYGLLAQNIPAQPKLVVGVVVDQMSYEFLY
                     RYTEQYSENGFKRLLKEGFSCENTHYSYIPTYTGPGHASIYTGSIPGVNGVVSNDWYD
                     ESTKQFMYVTQDNSVTAVGAAAGNQQSPKNMLTTTITDMLQLSNQQASKVIGIALKDR
                     GAILPAGHTADAAYWYDGSANAWVTSSYYMNALPAWVQSFNNEKQAEGMMNKNWNMML
                     PLSSYKSATADSVSWETVYIGEASPSFPHYMVYSGNSENIKATPFGNTLTANFAKAAI
                     ANEDLGKDAITDFLCVSFSSTDYVGHAYGPHSIETMDTYLRLDRDLADLINYLDTQVG
                     KGNYLMFLTADHGVSPTPQYMQHLKIPAGTTTEAIMTANMTTVLREQLGEGEWIKAYA
                     NQQIYLNDSLLLAKKISKKEIYNILYEPLMKLNGVAYVLLVDELHGATLNSKIKELVI
                     NGIYPKRCGDIQILYEPYWFEAYRPTGTTHGSHFSYDTHVPLVFYGWKMKPGALFRDT
                     YVTDIAPTIAALLKISEPNGCTGTIIPEVVK"
BASE COUNT         2881 a         1715 c         1528 g         2875 t
ORIGIN      
        1 accggccgta tcgcccggca aagcaccccc ggccagggca gcaccttcac cgtgtgcctg
       61 ccgcaaaaat aaattgtggt gtttaacaac attatcgcca atacaaaaaa aagtagcttt
      121 tatatggtag tctgatagga tatcgagcac tttatctgta acacccggaa ccggcccatc
      181 atcaaaagta aggtaaaccg ttttcgattt aggaatatcc caaaccaaac tcggatacac
      241 tgttttaaga taccagggtg ttttaacaaa atacatagat gcaaagttat ctttttgact
      301 aaaatttaag tattcggttt aacgcaattt gatatttaag ttaatcttta taacaaaaaa
      361 cataggaaaa tgattactaa tagctcaatt tacaatatac tgtaaccttt attacctggt
      421 gtattagaaa atggtgtgcc aattacgttg atatatgcca aactcagcag ttgaacagta
      481 ttttggacgg attaatacgc acattgatga ttatttgtac aagaaaaaga aggtcttaaa
      541 cgcttattag gaattaaaga aacggaccaa accagatata tacccaaagc tgtggtatat
      601 gtaattaaca tttaccaatg ggcaggaatt gagttggatg aagctactca tggtgcagga
      661 cactatttgc catatgccaa aatgggaagt taaatttaag tcatacaaaa tgttgttgat
      721 ttaattgaaa acagaaatgc tggtaatgtt ttggatatcc taaggttagc accctgtgtt
      781 attgaaaatt gcaagtttcg caaaaggata gccttgccta tttgttacaa cctacttaga
      841 acaaaaaaat cttacttttg accgcaatta tatggaaaag gtttatgagg taatgaaaga
      901 acggtcggtg tttattcccg tcctgaatac agcagggaaa tacatttttg aggatagtac
      961 cttttatgat gcaaatgcca ttcgaaaaaa atggaaactg aaaaaaaaat agaaatcttt
     1021 gtaaatttga ttgctaaaat tgaatcaatt actaatttta ccgagcagga aatagaaact
     1081 atcctaaaag catttatgga agacaattat cagggtatgg gtaaacggag gccaatattg
     1141 cgagcagccc tcgtagatag cttacaaaga ccaccggttt ttgctatggt agatgtatta
     1201 agaaaaaata attctgttgc ccgtttacat aattaaatta aaatttttga taactttcac
     1261 tgaaaattca gattaacctt atgcctaaga aaaaaccatt ggaaggtaaa cccgaagtgc
     1321 acaaagacct ggatggcttc gaaatgaaca ttaacgaatt tggcgagatt tcgtctaata
     1381 ccgatattga caaactcaac aaatttttaa acaaaaatgt tgacgacaaa aaattcaaag
     1441 gactggaaga cttgccttat gccaagcttg gtgatgacga tgatgatgat gccgttaaag
     1501 atgaggaaga tgatttaaat gaaatcgccg atgaatttga agagttccgt cagtttaatg
     1561 ataaggactt caaagatgaa ttcgactttg atgaaaaaga cgaaaaggaa gatttcggtt
     1621 ttgatgaagg taaacatatg tacgaagacc ttgatggtga cgatgataaa aaaattgatg
     1681 acgacgatga tgatgatttt gatgaggaag aatttgttga cgatatggcg gatgagtttg
     1741 gagaggaaga taactacaac tttgatgatg gcgatttcga tgacaggtat taaactttga
     1801 ccaaaacagt gtttccaaat atcatggcgt cggcatttgc cggcgctttt tttattaaaa
     1861 attcaagtaa aagtccttgg ttaatgcctt atacgcatcc gtgtaattgt tctgctcatc
     1921 ggcaatcaat aattcatttt ctacaacttc cattttttgt ttactaaatc gaagcaacag
     1981 gcgttttgct acaatacccg gtttggtaaa tacttgtgtt tttttacaac aataaagctg
     2041 ttcctgcagc gccgcagtaa taaacaattc accttcctgc tttggtaaaa caatccataa
     2101 attaccatca accgttaata attgttttgc tgcggcaatt aataaatcat gtggtaaatg
     2161 ttcagcatct ctcgctaatt ttcttcccgg attatcaatc tgccatcctt taataaaata
     2221 cggaggatta caaactatgg tagcgtaagt tgttgtacag cttaataaat actgttgtaa
     2281 atcaatagga tagactttga tttgattgta ccatgggcat aatgcaatgt tacctactgc
     2341 ctgctgaaat gcattttcat cccgttcaat agcatcaata cgtgcatcag gttttttttg
     2401 agctaacatg attgcgataa tacctgttcc ggtgcctata tccagcactt tttcgccaat
     2461 taaacgcgca tccacccatg caccaagtaa aacaccgtca gttcccactt tcatagccgt
     2521 ttcgccttgt tgtatagaaa attgtttaaa gtgaaaagcc gacataaatt caaaattcca
     2581 ttaaataatg ctatcaaatc cggagcaaat gtctaatttt agtagcaaat actttgtatt
     2641 atgaaaaaaa atctactcct cacatttgtt tcaatagctg ccctaagcaa tatcaacgcg
     2701 cagttaattg taactgaaga acccaacgac accactttta tttattcttt agctggtccg
     2761 ggaattgtag tttccggtat tgaaaggaat tgtgctgaag gcgcctcagg tttttttgat
     2821 gcaacttcag cgaatgttgg aattgataat ggtatagtgc tcacttccgg tactttacta
     2881 aatgtggttg gacctaacaa tttaagttca gcaaccggtt ataatggcac tgcaggtgac
     2941 gacgatttaa cgccattaac tatttgggaa acatacgatg catgtgctat ttcatttgat
     3001 atgaatgttg cagctgatac cctgaaattg cagttcgtat ttggctcaga agaatatgcc
     3061 gaatttgttg gttcaggatt taatgatgta tttgcatttt gggtgagtgg tcctggtatg
     3121 cctgatccgg ttaatattgc cgttgttccg ggaacagaaa ctcctgttac aatcaataat
     3181 ataaatattg ataccaatcc tgattattat atatataacg gcgatggcta ttcagaccca
     3241 tatgcatcag accctgctta tgttcagtat gatggactca ctacagtaat gctggctacc
     3301 attcctgtaa cagcaggcga aacttatcat atgaaaatga tagttgccga tgcaggtgat
     3361 gctattcttg attccggtgt atttcttgaa acgggttcat taggaagttt aagaatgcaa
     3421 tatcaaactt tggctgataa tgatttaacc aatgcagttg aaaaatgtgc taaaggttat
     3481 ttcaagttaa ttaatgaagt accttgcgct gacccccttg taattgatta ttatattgca
     3541 ggtagtgcaa caaatggtgt tgactatgaa atgttaccgg gacaaattac cattccggct
     3601 tttgtaagcg aaggtattat tgaagttaat cctattcctg atgctgttga cgaaagtttg
     3661 gaaagtgtgg tattgtattt atttaatcca caatccggat ttgtttacga tacactcaca
     3721 ctgttaatcg acgatgaagc agcacctgct tcatttacag ctgaaaacga tgatttagtt
     3781 tatacattta ccgaaacatc aggcattgca agcagcgtaa cttgggaatt tggtgatggc
     3841 attacttcta cggaaatgaa ccctacacat acttatttaa ctgaaggtgt ttatactgtt
     3901 tgtgttacct caataaatat ttatggttgc agcgatacct actgtgaaga agtaatggtt
     3961 ggagcggttg gaattaataa tcttagtaac gatttaacca tttcacctaa tcctgcgaac
     4021 aattatatta ctgttaatta ttccggtgta ataagcagcg acttaaccat caccattaaa
     4081 aatgtaacag gaacaattgt ttccacaacc acaagtaatg caccaactac aactttagat
     4141 gtacaaagtt tagcaagtgg catttatttt atagaattaa ataatggtgg cgttatttca
     4201 gttcaaaaaa tagaaaaatt ataaattata ctacaaaaaa aatcccgctt cagtaattga
     4261 agcgggattt ttttttcctt tgaattattt tgtaacaacc aatttttcaa caaactggtt
     4321 gccatttcct ttaatacgta aaaagtaatt gcctgctggt aaggtgctga tattgatgag
     4381 aaaagcaaca tctttattta ttgcagcatt tttggataat acggttttac cctgcatatt
     4441 aattatttca attgttgcat caggtaaaaa catattgagt tttaatctga ctacatcgct
     4501 cgcaggatta ggatataatt gtaaaactcc gttttcgttt aaattgttta ctgcaatatt
     4561 aggtgtttcg tcctccagct taaattccca ggtacctctt ccgtaggtgc cgaatcgggc
     4621 agcttgaatt tcatcaacaa aatcaacact ccaataagtt tgcagtggcg catcaaatcc
     4681 tgccaaatcg taccatttat tttctgcttt aacatataca tatggtgcaa cttcagttgc
     4741 tgcaaataac aaactatcat cggtcgaaat ggctaagtcg taaactaagg ttgatggcat
     4801 accatctgtt aatgcagtaa acgattcgcc gtgatttgat gacacatata cagcaggatt
     4861 atcgtaaccg cttccgccaa tatatactac atcagcatca gtttttgatg gttcaatagt
     4921 tgccccataa aaataatgag agcctggtcc ggaaaaactg gatgtcatcg tccacgtgtc
     4981 acctccattt tcggagtaat aaaaatttcc acctgaggtt aataaatacc agtattcagg
     5041 atttacaggc gaataattaa tcgctgaaat agaaccgcct ccggcaacgg caaaattttt
     5101 aatttgttta acacctgtaa tatcagcacc acttttttct aatcgccata aataagcacc
     5161 acctccttcg gagccaccac cccaccaggc aacttcatct tcatatgggt cagccataat
     5221 gggtgccatc cataaatgtc cggttcccac aaaatcccaa aaaaacatat ctgaacctga
     5281 ggctgcatcg ttaatataca tcgcaaatcc cgggtatacg caccacaaat tatctcctcc
     5341 gctgcgcgat actatatgtc cataatcgcc acttattaat tggtcgaaat aataatcatt
     5401 atccacttca taagtactgc gttgataccc ttgatcctga gcgccggcaa aaataatatc
     5461 cggcacaaaa cgataggtgt aaacatcata atactgcgca ttacgcattc cttcgttggt
     5521 tatattattc caaattaatc cattgttagt cgatttaaat aagccaccat ccgtactcac
     5581 caacactatt tcatcaccac ccgctggatt attgtaagtc ctgatatagg gaatatctgc
     5641 atgtaaatta tactccacat catcataata ttcccaccag taattattgc gcacccaggt
     5701 atctccaccg gtaaaacttc tataactatc tactccaccg tagtataaat acccctctgt
     5761 tttttgagaa caattaaaac tgtttttact aaaattgccc tcatccactt caccggttgc
     5821 tgaccagttt acaccagcat caaccgattt ataaaatttt gttttaccaa gtgcataatt
     5881 attatgtgca gcatataatg ttgtatttgt taatcctgta aacccacaca gcataatatc
     5941 accatcatca gtaatcggta aaatacctgt tgttgttaaa acacctgttg catcaatagt
     6001 aattactgta tcgttgtgaa taaaataact aatcgggtca ccattcactg cagaccaaag
     6061 tacaaattta tttggaccgc cataggttgg cacatcgtac gaagtaattt tttcaaacga
     6121 ctctcctaaa ttatctgact tataaataga aacgatatca taccaattag tgtaatccca
     6181 ttcgtatgct aaaaccagca tagtatgttc agcattattt aaaatttcgg catcaaatac
     6241 ataaccccat gttgccacat tttccatacc tgttgctgaa gtccagctaa ggccttcatc
     6301 ttcggtataa aacacccctt gagttcccca agctgatgaa gcagcaatta aacgatcatc
     6361 cattttgcgc aacatatgtg tgccggttaa tttaaaatta tcgtttatta cactccaatt
     6421 atcgccattt aaatcacctt tccataaatt ccctccgtcg gtaaaagcat aaatattatc
     6481 attttcaaaa tcaatatctg ttgcccaaat tcttcctgaa gtattaaagc tgcctatctc
     6541 atcccattta ccaattacat tgccctcagc caatgtatcc cattcgccac ctgttgtgcg
     6601 atatttataa tagttagcac gttccaacat ttttttatat cgcaagtcgg catccatttt
     6661 catccaattg gttccgggtg cagcattatg cattaaattt acgaatgctt ctcgttcttc
     6721 tttattttta tcctcatcgt tgcgatgttc cattacccga gtaggttgtg gtggttctga
     6781 ttgatggaaa ttattaccat ctttttgttt ttggaaaaca actgccataa ctataaaagt
     6841 taaaagaatt gtccccgcaa caatgagtaa tgcaattttc tttttcatgc cgtgttttta
     6901 ataattacaa tttattttac aacttcagga ataatggttc cggtgcaacc gtttggttca
     6961 gatattttaa gtaaagcggc aattgttggt gcaatatccg ttacataggt atcgcgaaac
     7021 aaagcgcccg gtttcatttt ccaaccataa aatactaaag gaacatgggt atcataacta
     7081 aaatgtgaac catgcgtagt acccgttggg cgataggctt caaaccaata tggttcatat
     7141 aaaatttgta tgtcaccaca ccgttttggg tatataccgt ttatgactaa ttcttttatt
     7201 ttactattta aagtagcacc atgtaattcg tcaactaata acacataagc aacaccgttc
     7261 aatttcatga ggggctcata taatatgttg taaatttctt ttttggatat tttttttgct
     7321 agcaataaac tatcatttaa atagatttgc tgattagcat atgctttaat ccattctccc
     7381 tctcctaact gttcacgcaa aacggtggtc atattagcag tcataattgc ttcggttgtt
     7441 gttcctgcag gaatttttaa atgctgcata tattgtggtg taggagaaac accatgatca
     7501 gctgttaaaa acattaaata atttcctttc cctacttggg tatctaagta attaattaaa
     7561 tctgctaaat ctcgatctaa acgtaaatac gtatccattg tttctattga atgtggtcca
     7621 tatgcatgac caacataatc tgttgatgaa aagcttacac ataaaaaatc ggtaatagcg
     7681 tctttgccta aatcttcatt cgcaattgct gcttttgcaa aatttgctgt taatgtattt
     7741 ccgaaaggag tagctttaat attttcacta ttgccactgt ataccatata atgaggaaat
     7801 gatggtgagg cttcacctat ataaacggtt tcccaactca cactatctgc tgttgcactt
     7861 ttatatgatg acaaaggaag catcatattc cagtttttat tcatcatgcc ttcagcttgc
     7921 ttttcattat taaacgactg tacccaggca ggaagtgcat tcatataata cgaactggta
     7981 acccatgcat ttgcagatcc atcgtaccaa tacgctgcgt ctgcagtgtg acctgcaggt
     8041 aaaatagcac ctctgtcttt tagtgctatt ccaattactt tagaggcctg ctgattactt
     8101 aattgcaaca tatcggtgat tgtagtggtt agcatatttt tcggtgattg ctggttgcca
     8161 gctgcagcac caaccgcagt aacagaattg tcctgagtaa catacataaa ctgtttagta
     8221 gattcatcgt accaatcgtt ggaaacaacg ccatttaccc ctggaattga gcccgtataa
     8281 atactggcat gccccggacc ggtatatgtt ggaatgtagc tgtaatgtgt attctcgcaa
     8341 ctaaaaccct ccttgagcaa tcgtttaaac ccattttcgc tatactgctc agtatatcgg
     8401 tataaaaact cgtaactcat ttgatcaact acaacaccaa ccactaattt aggttgagca
     8461 ggaatatttt gggctaataa cccataactc aaaagcatca acaataaagg tgtcacaata
     8521 tttttcatca ttatcctaaa atttttgata aagttaatta ctagtatcca tttaatcttt
     8581 acccattttg caaaatgccc tattttatac cctaaaatac ccctttaatt gcgacgaaat
     8641 cagtggtaaa gcaattagtt atgttgttta acctattcaa aaccgattta aacctgagca
     8701 ctttaaacga ttatagaata atacaaaccc tatttcaaac aagggtatta tttcaataca
     8761 attaagtaat gattcaaaca aagaaattta aaatgcagta aattgacttg ggattatata
     8821 aattgctgaa aaatcattgt tttcaatact ttaacaaaaa attccatatg taaatttaat
     8881 ttaaatttaa tttaatgctt ttgccaacat gctttgtaag cgtatctttg aaatgataaa
     8941 taaccaaagt caatctatta atattattgc agtaacatta aattgtgtca attaataat
//
LOCUS       DAORKW010000083         8872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_203_length_8872_cov_5.231144, whole genome shotgun sequence.
ACCESSION   DAORKW010000083 DAORKW010000000
VERSION     DAORKW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203_length_8872_cov_5.231144"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLB46_05025"
     CDS             <1..298
                     /locus_tag="PLB46_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75913.1"
                     /translation="MGENHFATNTVAVYSAILLMCGAAYYILQRCIMAGYKESSPMIE
                     ALQKQTTKANISTISYFSAIPLAFVHPGISGVLIVFVAALWLIPDKNIEKALDN"
     gene            complement(300..2588)
                     /locus_tag="PLB46_05030"
     CDS             complement(300..2588)
                     /locus_tag="PLB46_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF75914.1"
                     /translation="MSYCLATASKALSIKIKDKLKQTDYFYVFIKMKRLSSILLVFLL
                     MVGTNAYNQTICDPAGNVMLYSNYDGGTLNINVDVDIPNLKIGITTYEIVDVNIFGTF
                     VGNITEVIFAGFNAPAAIDGVDPALVTIYSTTTDNIAITNYLGDEIIPGFPPLVNCMV
                     SGSGCSETETGGGNSSPQIVQFYLAEFGPGSILYGHWTDYSVFPAGDFLVSEGGNCCL
                     EDPVTDPNPIYVGGADYNLLPDTTLLCDDEILLDVSFYPVVWGDPEWNTGDVGYTLLV
                     DEPGMYIVSISDYCHYDPGSYLLTDTIYIEPCATTIDTAICEGESFTLPDGSIVTAAG
                     EYEITLTAIDGSDSIVFVNLSLLPVYDILLNDAICDGLVYTLPDGTTTTTTGIYIFNF
                     ITAAGCDSIITLNLTVSTSYSTTINASICDDENYILPDGTLVSTAGTYVNNFTTVAGC
                     DSIITINLIVYPNYTILFDTTVCVGENVILPSGITTNIPGTYTTTFTTISGCDSTINY
                     TLNNFLPTTVTDEIPEIVCLESPPIELIVSLPGGVFSGIGVSGTTFDPAAAGIGGPFS
                     ISYSVNDANGCITGGVYPIIVTQNYADAGSDTTIFDTGVAELNATAGGSVTWSPAEGL
                     TCSNCFTTYSDVDYTTTYSLVSIDPNGCIATDEVTVFVITSNENIYIPNTFTPNGDGV
                     NDAFSILGPGIVLIHTCAIYDRWGELIFYTENTSPNQPNALWDGTFKGTDVNQGVYAY
                     TTLVELATGRKVQLKGNVTLIR"
     gene            2548..3339
                     /locus_tag="PLB46_05035"
     CDS             2548..3339
                     /locus_tag="PLB46_05035"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF75915.1"
                     /translation="MESAFDAVAKQYDNEFSYSAVGKAQRACVWRHLGAILPSKKVQI
                     LEMNCGTGEDAIRFIKLGHSVLLTDVSNEMVTTATNKLEQEGLTNFTTSIWNLKDTFP
                     LASDKYDLIFSNFGGWNCLSKSELMALATQCEKLLSRDGKIVAVVMGRKCLWEKWYFR
                     FKHKPEEQKRRLLTTPSKARLDKVTAVDTWYYSPDEIAQLFSGFAVTKIKPIGLFIPP
                     SYLNPFFSHKLFLLKILTRLDKLFSFSKLSNYADHFLIVLEKKNN"
     gene            3503..4873
                     /locus_tag="PLB46_05040"
     CDS             3503..4873
                     /locus_tag="PLB46_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF75916.1"
                     /translation="MKVLLTHGYFIADDPKEQVIMRPYVPLGILYISGFLEKQGVEHE
                     VFDSTFQTQELQLAFIEKNQPDFIGIYVNLMTKVNVLKIITAVKKMPSLQHCKIILGG
                     PEVRNHVDNFLKHGADVIVLGEGEITFHEVINTLHEKKDLLTVKGICYKNTINEIIHT
                     AEREKIKDLDVLPVPNRKKVNLQLYLDAWKKHHGKNAISISTMRGCPYTCKWCSRAVY
                     GLSYRRRSAANLVDEMEQLKTTYQPDTLWFVDDVFTVSHNWMQAFANEVQLRNCKIPY
                     ECITRADRLNEEVLQLLQQSGCIRVWIGAESGSQKIIDAMDRRVDVEQVRNMIQLAKK
                     YHIETGTFIMLGYPGEDERDIEETIFHLKAANPDYFTITVAYPIKGTALYTQVEPDFI
                     NQQDWTTSSDRDINFKRTYPEQYYKFAVRRVVNEVNYFKHKRLQDKVKSSLAKSGMWM
                     CRTFLS"
     gene            4870..5928
                     /locus_tag="PLB46_05045"
     CDS             4870..5928
                     /locus_tag="PLB46_05045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRF75917.1"
                     /translation="MNDKILILTPGFPENEQDSTCVPYIQQYVIALAKKIGNENVVVV
                     SLQYPFTRAPYIWNGIQVFPSGGKNNKSPFAYYLNLRRTRKRISKLYKTAHFCVHAFW
                     MGEAAFLGRYIAKKYISRFVVTFMGQDVNQGNRVMRFIDYSKTFIVTLCRFHDDTFFA
                     NYGHQADAIIPLPIKAHPFQTDSLRPIDILFVGSFIPVKQPLQFVEIISRLKSSYPNI
                     NAVMIGEGNLMEEVKTEISKLQLDNNIRLLGQVSHDVVFDTMSKAKILLHTSKFEGQC
                     LVYAEALSLGMYVVSYPVGWFDSSAKHITGKSVEELADKVQHLFSQPLSFHQEQVINT
                     DNIVDAYLKLYWGGQENV"
     gene            6002..7432
                     /locus_tag="PLB46_05050"
     CDS             6002..7432
                     /locus_tag="PLB46_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF75918.1"
                     /translation="MAASIDSLFPYVIVDGNLEEDPLQKIEQYVLSGNFKYFALTVMP
                     GPQLKQAIPFSKKIKSAFPHITIIWGGYFASNQHKVVMESGYIDYIIYGPGDKAFPQL
                     LSSLENNAPLHLIPNLIWNEEGNIIKNHKDELYEQDELPPLPYEKLNSFYPISKYLGK
                     TYLGTKTIAYHSSIGCPFTCAFCGIVPIYNARWKGKSASLIYQDIQYLKNNYGGNAIE
                     FHDNNFFVSEKRCVEFSKMIMHDNIQWWGEGRIDTLDKYSDESLLLMHKAGCKMIFFG
                     AETGNDKILKMMDKGGTQTGEQIKRFAARLHRIGIVPEYSFVLGTPGESVEATEKQIE
                     EDIQFIKTIKSINPDTEIIIYVYSPVPTEGSDLYMAVQQTGFHFPQKLEDWISPQWEN
                     FDLRKNPLTPWLSPGMIDKIKNFETVLNGYFPTVSDIRLTHRKRKIISTIAAWRYKSG
                     FYALPYEIKFLQKYWLQYQQPETEGF"
     gene            7432..8088
                     /locus_tag="PLB46_05055"
     CDS             7432..8088
                     /locus_tag="PLB46_05055"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRF75919.1"
                     /translation="MRRISKKFTQWFLQPIISRRISRASEYTYNGITLHIPAGVFHPK
                     YFFSTKYLLKQLTTLPLQGKTFLELGAGNGLISISAAKHGAQVTASDISDLVVKTLVQ
                     NALDNNVKINIIKSDLFQQIPKQTFDIIAINPPYYPKQARNDLEKAWYCGVNFEYFYA
                     LFREIKDYMTTSSIVLMVLSDGCDIQGITTIANKNGLKLALYHQQQIWWEREFIFRIL
                     "
     gene            8156..>8872
                     /locus_tag="PLB46_05060"
     CDS             8156..>8872
                     /locus_tag="PLB46_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75920.1"
                     /translation="MKVLHRSATEKIQLSPATSAVFKVLAYFDVFKYPLTLPEIEKYA
                     TFRFHHLSEVEEALGFLTERLIIFQFGEFYSLRNEFCLITRRTTGNLAAFNIYKKAQK
                     RAALIHQFPFVRSVNVSGSLSKNYFDDSTDIDYFIIAEPGRLWLCRLLLTFYKKVFLF
                     NSRKYFCINYYIDSNHLEIPDKNLFSATEIVTLRNMSGGDYYERFVSANNWVNDYFPN
                     HHEDYPVGSITNDTRIKKVLE"
BASE COUNT         2962 a         1468 c         1676 g         2766 t
ORIGIN      
        1 gatgggtgaa aatcatttcg caactaatac agttgcagta tatagtgcaa ttttattaat
       61 gtgtggggca gcctattata ttttacaacg ttgtatcatg gcagggtata aagaatcgtc
      121 accaatgatt gaagcactac aaaaacaaac aacaaaagcg aacatttcaa ctatcagtta
      181 ttttagtgca attccgttgg catttgtaca tcctggtatt agcggagtat taatagtttt
      241 tgtagctgcc ctttggttaa tcccggataa aaatattgaa aaggctttag ataattgatt
      301 tatcgtatta atgtaacatt accttttaat tgtacttttc tgcctgttgc taattcaacc
      361 aatgtggtat aggcatatac accttgattt acatctgtgc ctttaaaagt gccatcccat
      421 agtgcatttg gttggttggg agatgtgttt tcggtataaa aaattaattc tccccaacga
      481 tcataaatgg cacatgtatg aatgagtact atgcccggac caagaatgga aaatgcatca
      541 tttacgccat caccatttgg agtgaatgtg ttgggaatat aaatgttttc gtttgatgta
      601 ataacaaata cggttacctc atcagttgca atacaaccat ttggatcaat tgaaactagt
      661 gaatatgttg tagtataatc aacatcgcta taagtagtaa aacaattgga acatgttaga
      721 ccttccgcag gcgaccacgt tacggaacct cctgcagtag catttaattc tgcaacaccg
      781 gtatcaaata tagtagtgtc ggaaccggca tctgcataat tttgagtgac aattatcgga
      841 tatacgccac ctgtaataca tccattggcg tcgtttaccg aataagaaat agaaaacgga
      901 cctccaattc cggctgcagc tggatcaaat gtggttccac ttacaccaat acctgaaaat
      961 acacctcccg gaagcgatac aataagttca attgggggcg attctaaaca aactatttcc
     1021 ggaatttcat cagttactgt tgttgggaga aaattattta aagtataatt aattgtacta
     1081 tcacaacctg aaatcgtagt aaatgttgtt gtataagtac cgggaatatt tgttgttatt
     1141 ccactcggaa gaattacatt ttcgccaacg caaacggtag tatcaaaaag aattgtataa
     1201 ttcggataaa caataaggtt aattgtaata atactatcac aacctgctac agtagtaaaa
     1261 ttatttacat aggttccagc tgttgataca agtgtgccat caggtaaaat ataattttca
     1321 tcatcacaaa tacttgcatt aattgtggtg gagtaagaag tagaaacagt taaattcaag
     1381 gtaataatgc tatcgcatcc tgcagcagtt ataaaattga aaatataaat tccggtagtt
     1441 gtagttgtgg ttccatcggg aagtgtgtaa actaatccat cacaaatggc atcattcaac
     1501 aagatatcgt aaactggcaa caatgataaa ttaacaaaca caatactatc actaccatca
     1561 attgctgtaa gggtaatttc atattctcct gcagcagtta caatgctacc atcaggcaat
     1621 gtgaaacttt caccttcgca tatagcagta tcaatagttg ttgcacacgg ctcaatataa
     1681 atagtatcgg ttaataaata ggaacccggg tcgtaatggc aataatctga aatggaaaca
     1741 atatacattc ccggttcatc aacgagtaaa gtataaccaa catctcctgt attccattca
     1801 gggtcgcccc aaactaccgg ataaaatgac acatccaata aaatttcatc atcgcataat
     1861 agtgtagtat cgggcaaaag attataatct gctccaccaa catatattgg attcgggtcg
     1921 gtaacaggat cttctaaaca acaattccct ccttcactca ctaaaaaatc tccagcagga
     1981 aaaacactat aatctgtcca atgcccataa agaattgaac cgggaccaaa ttctgccaaa
     2041 taaaattgca caatttgggg agatgaattt ccacctccgg tttctgtttc gctgcaacca
     2101 cttccggaaa ccatacaatt aactaatgga ggaaaaccag gaattatttc atcacctaaa
     2161 taattggtaa ttgcaatatt gtctgtagtt gtggagtaaa tagtaacaag ggctgggtca
     2221 acaccatcaa tagctgccgg tgcgttaaat cctgcaaata tgacttcagt tatatttccg
     2281 acaaaggtgc caaatatgtt tacatcaaca atttcgtaag tggttattcc aattttgaga
     2341 ttgggaatat caacatctac atttatattt aaggtgcctc catcgtaatt agagtataac
     2401 ataacgttgc cggctgggtc acaaatagtt tgattataag catttgtgcc aaccattaac
     2461 aaaaacacta acagtatgga tgataaccgt ttcattttaa tgaaaacata aaaataatct
     2521 gtttgtttta atttatcttt aatttttatg gaaagtgctt tcgatgcagt agctaaacaa
     2581 tatgacaacg aattttccta ttcagccgtt gggaaagccc aacgcgcttg tgtttggcgg
     2641 catctagggg caattctgcc atctaaaaag gtgcaaattt tagaaatgaa ttgtggaaca
     2701 ggtgaggatg caatcaggtt tattaagctg ggtcattcag ttttgctaac cgatgtttca
     2761 aatgaaatgg ttactacagc tacaaataaa cttgagcagg aagggctaac taattttact
     2821 acaagtattt ggaatttaaa ggatacattt cctttagcat ctgataaata tgatttaatt
     2881 ttttccaatt ttggaggatg gaattgttta tctaaaagtg aattaatggc actcgccact
     2941 caatgtgaaa aattattgtc acgtgacggc aaaatcgttg ctgtggttat gggaagaaaa
     3001 tgtttgtggg aaaaatggta tttcaggttt aaacataaac ctgaggagca aaaaaggcga
     3061 ctacttacaa ctccatcaaa agcaaggctg gacaaggtta cagcagttga tacctggtat
     3121 tattctcccg atgaaattgc acaattattt tcgggttttg cagtaacaaa aataaaaccc
     3181 attggtttgt ttatcccgcc aagttacctt aatccgtttt ttagccataa attattttta
     3241 ttaaaaatat tgaccagatt agataaatta ttttcttttt caaagttaag taactacgcc
     3301 gaccactttt taattgtttt ggaaaagaaa aacaattaat taccaaattt acttacttct
     3361 tacaattttg gttataatgg ttttcaattt cactgtttga ggggtgccat ccatggattc
     3421 tatcccatga agtatgacgg gaaaatttta tagatatgta cttttagtgt agcttgtgat
     3481 taaatttgtg tttatcaggc ttatgaaagt gcttttaacc cacggctatt ttattgcaga
     3541 tgaccctaag gagcaggtta tcatgcgtcc ttatgtgccc ttgggaattt tatatatatc
     3601 cgggtttctt gaaaaacagg gtgttgaaca tgaggttttc gacagtacat ttcaaacaca
     3661 ggaattgcaa ttggcattta ttgaaaagaa tcaacctgat tttattggta tttatgtcaa
     3721 tttaatgacc aaagttaatg tgctgaaaat aataacagcg gttaaaaaaa tgccatcatt
     3781 acaacactgt aaaattattt taggcggacc tgaagtgcgg aatcatgtcg acaatttttt
     3841 gaaacatgga gcagatgtta ttgtattagg tgaaggagag attacttttc atgaggtaat
     3901 taatacgtta catgaaaaga aagatttatt gacggtaaaa ggaatatgtt ataaaaatac
     3961 gattaatgaa ataattcata cggcggaaag agaaaaaata aaagatttag atgtgctccc
     4021 tgttccgaac cgcaaaaaag taaatttaca gttatacctc gatgcatgga aaaagcacca
     4081 cgggaaaaat gctatttcaa tttctaccat gcgtggttgc ccttatactt gtaaatggtg
     4141 cagcagagca gtatatggtt taagttatcg tcgacgtagc gctgccaatt tagttgatga
     4201 aatggagcag ttgaaaacga cttatcaacc cgatacactt tggtttgtag atgatgtatt
     4261 tacggtgagt cacaattgga tgcaggcctt tgctaatgaa gtacaattaa gaaattgtaa
     4321 aattccttac gaatgtataa cacgtgctga cagattgaat gaagaagttt tacaattatt
     4381 gcagcaaagc ggttgcatca gagtctggat tggagcagaa agcggttcac aaaaaataat
     4441 tgatgcaatg gacaggcgcg tagatgttga acaggttcgc aatatgattc aactcgcaaa
     4501 aaaataccat attgaaaccg gtacgtttat tatgctcggt tatcccgggg aagatgaacg
     4561 tgatattgaa gaaacaatat ttcatttaaa agcggctaat cccgattatt ttactataac
     4621 tgttgcatat cccattaaag gaacggcact ttatacgcag gtggagcctg attttattaa
     4681 ccaacaggat tggactactt ccagtgatcg tgacattaat tttaaacgta cttaccccga
     4741 acaatattat aagtttgctg tacgacgagt tgttaatgaa gtaaattact ttaagcataa
     4801 acgtttgcag gataaagtaa aatcatcgct tgcaaaatca ggaatgtgga tgtgtcgcac
     4861 ttttttatca tgaatgacaa gatactcata ttaacaccgg ggtttcccga aaatgaacag
     4921 gacagcacct gtgtgccgta cattcaacaa tatgtcattg cattggctaa aaaaataggg
     4981 aatgaaaatg ttgtggtagt cagtttgcaa tatccgttta cccgagctcc ttacatttgg
     5041 aacgggattc aggtatttcc atctggagga aaaaataaca aatcaccttt tgcttattat
     5101 ttaaatttgc gcagaaccag aaagcgtata tcaaagttat ataaaactgc acatttttgt
     5161 gtccacgcat tttggatggg tgaagctgca tttttaggca ggtatattgc taaaaaatat
     5221 atttcaaggt ttgtggtaac atttatggga caggatgtta atcagggtaa tcgcgtaatg
     5281 cggtttattg attactccaa aacatttata gttaccttat gtcggtttca cgatgatact
     5341 ttttttgcca attatggcca tcaggcagat gccataattc cgctgccaat aaaagcgcat
     5401 ccgtttcaaa ctgatagcct gaggcctatt gatattttat ttgtcggctc atttattccg
     5461 gttaaacaac ctttgcaatt tgttgaaatt ataagtaggc tgaaaagcag ttatccaaat
     5521 attaatgctg ttatgattgg agaagggaat ttaatggagg aagttaaaac ggagatatca
     5581 aaattgcaat tggataataa catcaggtta ttaggacagg tttcgcatga tgttgtgttt
     5641 gatacaatgt caaaagcaaa aatattatta catacttcaa agtttgaagg acaatgtctg
     5701 gtgtatgctg aagcattatc actcggtatg tatgtggtca gttatccggt aggctggttc
     5761 gattcctcag caaagcatat taccggaaag tcggttgaag aacttgcaga caaggttcaa
     5821 catttatttt cacaaccgct tagtttccat caagaacagg taatcaatac agacaatatt
     5881 gtggatgcat atttgaaatt atattgggga gggcaagaaa atgtataata aaaatggcgt
     5941 tatattattt aaccctcgag cggctaacta caagcctcgc attccgaatt ctgttttgtc
     6001 ggtggctgct tctattgata gcttgtttcc ttatgtaatt gttgatggta atttggaaga
     6061 agaccccttg caaaaaattg aacaatatgt attatccggt aatttcaaat atttcgcatt
     6121 aacggtaatg ccagggccgc agctgaagca ggcaatacca ttttccaaaa aaataaaatc
     6181 ggcatttcct catatcacaa ttatttgggg cgggtatttt gcgagtaatc agcacaaagt
     6241 ggtaatggaa tcggggtata tagattatat tatttatggt ccgggcgaca aagcctttcc
     6301 gcagctgtta tcctctctgg aaaataatgc accattacat ttaattccaa atttaatttg
     6361 gaatgaggag ggtaatatca taaaaaatca taaagatgaa ttatatgagc aggatgaatt
     6421 gcctccttta ccatatgaaa aactcaatag cttttatccg atttccaagt atttaggtaa
     6481 aacgtatttg gggacaaaaa ctattgctta tcatagcagt attggttgcc cgtttacttg
     6541 tgctttttgt ggaatagtgc caatctacaa tgccagatgg aaagggaagt ctgcttcgct
     6601 gatatatcag gatattcaat accttaaaaa taattacggc ggaaatgcaa ttgaatttca
     6661 tgacaataat tttttcgttt cagaaaaacg ttgtgtggag ttttcaaaaa tgattatgca
     6721 tgataacata caatggtggg gtgaaggccg tattgatact ttagataaat attcagatga
     6781 aagtttgcta ttaatgcaca aagcgggatg taaaatgata ttttttggcg ctgaaaccgg
     6841 caacgataaa atattaaaaa tgatggataa agggggcact caaaccggcg aacaaataaa
     6901 gcgatttgca gcacgtttgc atcgaattgg tatagtgccc gaatattctt ttgtattagg
     6961 aactccgggt gaaagtgttg aggcaactga aaaacaaata gaagaggata ttcagtttat
     7021 taaaaccata aaatccatta atccggacac ggaaattatt atttatgttt attccccggt
     7081 gcccactgaa ggaagtgatt tatatatggc tgtgcaacaa acgggttttc atttccccca
     7141 aaaattagag gactggattt caccacaatg ggaaaatttc gatttaagga aaaacccatt
     7201 aacaccgtgg ttaagtcccg gtatgataga caaaatcaaa aatttcgaaa ccgtactaaa
     7261 tggctatttc cctacagtaa gtgatatcag acttacacat cggaaaagaa aaattatttc
     7321 aaccattgct gcatggaggt ataaatcggg attttatgca ttgccttatg aaattaaatt
     7381 tttgcaaaaa tattggctgc aataccaaca gcccgaaacg gagggatttt aatgcgtaga
     7441 atcagcaaaa aatttaccca atggttttta caaccaatta tcagtcgccg aatttcacgt
     7501 gcttctgaat atacgtataa cggcatcacc ttacatattc cggcaggggt tttccatcca
     7561 aaatattttt ttagcacgaa atatctgttg aaacaattaa caacattacc attgcagggg
     7621 aaaacatttt tagaattagg agccggcaac ggacttataa gtatatcggc tgccaaacac
     7681 ggtgctcaag taactgcctc tgatattagt gatctggtag taaaaacatt agtgcaaaat
     7741 gctttagata ataatgttaa gataaatatc ataaaaagtg atttattcca acaaatcccc
     7801 aaacaaacct ttgacataat tgctattaat ccaccttatt atcccaaaca ggcacgtaat
     7861 gatttagaaa aggcatggta ttgtggtgtt aatttcgaat atttttatgc tttattcaga
     7921 gagattaaag attatatgac tacttcgtca attgttttaa tggtattatc cgatggttgc
     7981 gacatacaag gaataacaac aattgctaac aaaaatggat taaaattagc actgtatcat
     8041 caacaacaaa tttggtggga acgagaattt atttttcgta tactgtaaca taatataacc
     8101 attgttatta tttatgtaat caaaaaaccg gtatcttagc atacggtaat tttttatgaa
     8161 agtattacat cggtcagcaa ctgaaaaaat ccaattatct ccggctacat cagccgtatt
     8221 taaagtgttg gcatatttcg atgtgtttaa atatccttta acgttgcctg aaatcgaaaa
     8281 atatgcgact tttcgttttc accatttaag cgaagtggaa gaagctttgg gatttttaac
     8341 ggaacggcta atcatttttc aatttggaga attttattca ttgcgcaacg aattttgttt
     8401 aattaccaga cgtactaccg gtaatttagc ggcttttaat atttataaaa aagcgcaaaa
     8461 aagagctgcc ctcatacatc aatttccatt cgtaagaagt gtaaatgtct caggctctct
     8521 ttcaaaaaat tattttgatg atagtactga tatcgattac tttataatag ctgagcccgg
     8581 aaggttatgg ttatgcaggt tgttgttaac attttataaa aaagtgtttt tatttaattc
     8641 aagaaaatac ttctgtatta attattacat cgattccaat catttggaaa ttccggataa
     8701 aaatttattt tcggctactg aaattgtgac cttacggaac atgagtggtg gtgattatta
     8761 tgaacggttt gtatccgcta acaattgggt taatgattat ttccctaatc accatgaaga
     8821 ttatccggtt ggatcaatta cgaatgatac caggattaaa aaagttttag ag
//
LOCUS       DAORKW010000084         8836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_204_length_8836_cov_5.325362, whole genome shotgun sequence.
ACCESSION   DAORKW010000084 DAORKW010000000
VERSION     DAORKW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204_length_8836_cov_5.325362"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..919
                     /locus_tag="PLB46_05065"
     CDS             131..919
                     /locus_tag="PLB46_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRF75921.1"
                     /translation="MNLVKNIPNLLTLFNLFLGCMGVVYLFNDHMVIMSAERDMFIDM
                     GQIHLACYCVFLAGFIDFFDGFIARLLKVQSELGKQLDSLADMVTFGLLPGLIMYELL
                     ARAYYANYNSFDYPILFYAAGFALTIFAAIRLGKFNLDTRQLNEFRGLPSPSMAFFIV
                     ALPLIILRDEMGLTNLLSNNWVLLLIIVALCYFMVSDIRMLSLKLKSLKFEENQWLYS
                     LVLLSLIIAFIGIFILHVYFVIIPVIILLYIIISIAKNISENGI"
     gene            909..1160
                     /gene="purS"
                     /locus_tag="PLB46_05070"
     CDS             909..1160
                     /gene="purS"
                     /locus_tag="PLB46_05070"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRF75922.1"
                     /translation="MAYKAEINVMPLKELLDPKGKAVAGSMKNLDLSSIQDVRIGKHI
                     QLHIEAGSKAEAEKIATEACKKLLANPIMESFEVVVHEA"
     gene            1235..1903
                     /gene="rsmI"
                     /locus_tag="PLB46_05075"
     CDS             1235..1903
                     /gene="rsmI"
                     /locus_tag="PLB46_05075"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRF75923.1"
                     /translation="MAKLYVVPTPIGNLEDMTFRAVRILSEVDLILAEDTRTSHVLLS
                     HYNIKTKTSAHHKFNEHAQVEKIAQDILDGKNVALISDAGTPGISDPGYLLVHTCVLK
                     NITIETLPGPTAFVPALVNSGFPTDEFVFMGFLPVKKGRMTKFKSIAEELRTIVIYES
                     PYRIIKTLEQLNIYCSDRTISISRELSKKFEETLRGTPEQLLQHFSQHEPRGEFVLII
                     AAKK"
     gene            complement(1904..3454)
                     /locus_tag="PLB46_05080"
     CDS             complement(1904..3454)
                     /locus_tag="PLB46_05080"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HRF75924.1"
                     /translation="MRKVQIFVIIFQQHLSNAAVYYLIRQLYQPTVGTHKPEHTHEQV
                     YSFDDFRLMPRGVLIIICFTVLVFSNTMAQEFIGLRTDNYSGSNGMLLNPATPIAGKL
                     PWDVNMMAFGVNEDNNYLKIDDPLLKYVTTSDSIIDVDFYNPHKLYGHGNVLLQLPSA
                     FVKFDDFAVGTFFTARTAGYALSTKDTMSVSGVLNVPIDSVLYMPQFNAAALIWGEVG
                     INGEFTLESNSVYALHLGANLKYLTGFEAVAFRNNDPFNYVKDTVNTTTPYFDADFDY
                     TRNLGSNLLYDPNNYALNGSGFGIDIGGYFVLHKKSKFKYSKKLDYTWKFGASLVDLG
                     VIKFKSNSGSYHLLQEEPLVVANLVLDSIGDIDEFNRTGSRIIYDNSDASKDASTFSM
                     FLPAALVLTADKPLPHNFYVQAMIVRRIPNFETNLIARSNLFALTPRYEGRKLGFTLP
                     MSLYEDKEFRIGAAARILFLTVGSDNLRTWFHEEDYASIDFYVGVKLTPYWLVPDTKK
                     RFLECLKW"
     gene            complement(3484..4884)
                     /locus_tag="PLB46_05085"
     CDS             complement(3484..4884)
                     /locus_tag="PLB46_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRF75925.1"
                     /translation="MYQPISTDVISQLAKIVGHDYVLLDGENLEKYAHDETEDLRYQP
                     EVVVKPGTSEEIAAIMVLANLHHFPVTPMGARTGLSGGALPVCGGVALSMERFNKIIH
                     IDERNLQVTTEPGVITEVLQNAVIEKGLFYPPDPASKGSCFIGGNVAENSGGPKAVKY
                     GVVKDYVLNLEVVLPTGEIIWTGANVLKNATGYNLTQLFVGSEGTLGIITKIVLRLIP
                     YPQKNLLMLVPFSNSTKACEAVAAIFRAGITPSAMEFMERDAIDYVLQFNTDIQIAIK
                     DHIKAHLLIEVDGNNQDVLMQDCETIAQIVEQFECDEILFAETEREKNDLWKMRRKVG
                     EAVKQHSTYKEEDTVVPRAELSVLLEGVKAIGNKYGFQSVCYGHAGDGNLHVNILKGT
                     LTNEEWESRLPQGITEIFQLVIKLGGTISGEHGIGWVQKKYMPLAFNEIQLNIFRNLR
                     KIFDPNGILNPGKVVD"
     gene            5024..7072
                     /locus_tag="PLB46_05090"
     CDS             5024..7072
                     /locus_tag="PLB46_05090"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRF75926.1"
                     /translation="MKKNIYLFLFGVILLSSNTLFAQAKKYPLFEHFTQASCGPCAAQ
                     NPFFQAVYVANKSNVHHIAYHTSWPGFDPMYNFNMSESDEMVDYYGVSGVPDMIVNGA
                     EFGSPAGVTQDVVNNLLTESSPIRVLVTETVAGSTVNVHIEVQTVGTIPAGTYKIKAA
                     AVERIIEYASAPGTNGELDFPNVFRESLTGTDGDAITPAAIGSSVSFDYSYDLAAEYV
                     AENMYAIAWVQNVSSKDILNSGASGDPQVEVVNTTTATFAQGTIGAANAFEGNIINIG
                     DANANIEITLSASQPADWSSNFVYNGTTYTTTASASINALSELPINLNVNVGATSGIG
                     EYTITVEFPDNPEFMPQVLSYYVISGVTDLIVNNEVSFGDGTPYGTYNWESLYTDALN
                     AAGVSTYAATSHFTMKKGFSSNSLDDIENIYYNVGWTFPGLVDDEIKIEQLMEFLDNG
                     GNLFISGQDIGWEVNEYAAFYPQAVDFYENYLHATYVNDAASGATTLTAVVDETWYGD
                     IAESEISKPYGTTYYYPEQIEANGAEAYDIFTYNAGTKVGGVRSETADYKTVYLGIGI
                     EMIEDINVRNAVMEATYLYFKGNIDGIAYDNIVKGLLGGAQPNPAVNATTITLSNLDK
                     NYVIYVTDITGKIVLEDNITAGTDSYNLNTSDLSSGMYFYYLTDGQQRTQTEKLNVVK
                     "
     gene            7096..7824
                     /locus_tag="PLB46_05095"
     CDS             7096..7824
                     /locus_tag="PLB46_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75927.1"
                     /translation="MKKLILLPAILFIATTSFAQIMLMPSEAEIEGDPTSINEVHIEF
                     HNMGEGQPMTWIRTVNDIPEGWKTSVCDFNLCWADFADEPDYTFSAPADTIGTVYVKF
                     DARNFHDGAFDPIPGCGTVEVNFYSVADSAGYNALGVFHARLGVEAEDCKTLIVTPIN
                     DNNFLIYPNPAKNVVNAVASFSADIQHAEIINTAGIVVRSYNWETTSGKMTFDLNDLP
                     AGIYFMRLVDVNNAGVYTEKVIIE"
     gene            8032..8451
                     /locus_tag="PLB46_05100"
     CDS             8032..8451
                     /locus_tag="PLB46_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75928.1"
                     /translation="MSAQVNDIKYPDIPVSEVIDNDKFTIYLRNDLIVYVLIKQNNFF
                     DVPDVTEVLEGIKKVSKGNIFPLIAVYETNIGFSKNAKDIVAKHHLTTADALVTLDNW
                     PIRVLANFYLKVNKPIRPTRIFGEETTAIEWLKLYRE"
     gene            8567..>8836
                     /locus_tag="PLB46_05105"
     CDS             8567..>8836
                     /locus_tag="PLB46_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75929.1"
                     /translation="MKKCIAALIAIMLSVAGFSQNVSINNDGSTADNSAMLDIKSTSK
                     GILVPRLTAAQKLGIATPATGLLIYQTDGTTGFWYYDGSNWVNLSS"
BASE COUNT         2820 a         1587 c         1605 g         2824 t
ORIGIN      
        1 agtggtcaac ccacgtgttg acctgacgat ttacggcgga atagggtagt ttgaggatag
       61 tgggatactt tatttataag atgaaccgtt gatgttttaa ttttgcgctt tataataatc
      121 gtataacctg gtgaatttag taaaaaatat acccaacctg ctaaccctgt tcaacctgtt
      181 tttggggtgc atgggtgttg tttatttatt taacgaccac atggtgatta tgagtgccga
      241 acgcgatatg tttatcgata tggggcaaat acacctggcc tgttattgtg tgtttttggc
      301 aggtttcatc gatttttttg atggatttat agccagattg ttaaaagtgc aaagtgaatt
      361 aggcaaacaa ctcgattcgc ttgccgacat ggttactttt ggcttattac ccggtttaat
      421 tatgtatgaa ttattagcca gagcttatta tgcaaattat aattcattcg attacccaat
      481 attattttac gcagccggat tcgcgttaac catttttgcg gctatacgat taggcaaatt
      541 taatttggat acccgtcagt tgaatgagtt ccgcggatta ccatcaccaa gtatggcatt
      601 ttttattgta gcgcttccgc ttattatttt acgcgatgaa atgggtttaa ccaacttact
      661 cagcaacaat tgggtattac tcctaattat agttgccctt tgttatttta tggtaagcga
      721 tattcgtatg ctaagcctga aattaaaatc cttaaagttt gaagagaacc aatggttata
      781 tagcctggtt ttattatcgc tcataatcgc atttatcggc atatttattt tacatgtgta
      841 ttttgtaatt attccggtaa ttattttatt atatattatt atttcaatag ctaaaaacat
      901 atcagaaaat ggcatataaa gcagaaatca atgtgatgcc tttaaaagaa ttgctcgacc
      961 caaaaggaaa agcggttgca ggcagcatga aaaatttaga tttaagttca atacaagatg
     1021 tacgcattgg caaacacatt caattacata ttgaagcagg tagtaaagct gaagcagaaa
     1081 aaatcgcaac agaagcatgt aaaaaacttt tagcaaatcc aattatggag agctttgaag
     1141 tggtggtaca tgaggcataa tgccatttat ttaccgtaca ttatttagca gcatcaattg
     1201 taatgttgct caacaaatat ttaattgcaa ttaattggca aaattatatg tagtacccac
     1261 tcctattggc aatctggaag acatgacttt ccgggcagtg cgtatattaa gtgaggtaga
     1321 tttaatttta gccgaagata cacgcacttc tcatgtgttg ttgagtcatt ataatatcaa
     1381 aacaaaaaca tcggcgcatc ataaatttaa tgagcatgcg caggttgaaa aaattgctca
     1441 ggatatttta gacggcaaaa acgtagcatt aatttcagat gcaggcactc cgggtatttc
     1501 tgacccggga tatttattag tgcatacttg tgtattaaaa aatattacta ttgaaaccct
     1561 tcccggccca acagcatttg ttccggcact ggtgaattca ggtttcccaa ctgatgagtt
     1621 tgtttttatg ggatttcttc cggttaaaaa aggaagaatg acaaaattca aatccattgc
     1681 agaagaatta cgtacaattg taatttatga atctccttat cgaattatca aaacactgga
     1741 acagttaaat atatattgca gcgatagaac aattagtatt tcgcgggagc tttctaaaaa
     1801 atttgaagaa acattacgcg gcacacctga acaattattg cagcacttta gtcaacatga
     1861 accacgtggt gagtttgtgc tcattattgc agcgaaaaaa taattaccat tttaaacact
     1921 ccaaaaaccg ctttttggta tcaggcacta accaataagg tgtgagttta acaccaacat
     1981 aaaaatcaat agaggcataa tcttcttcgt gaaaccaggt acgcaagtta tcagatccaa
     2041 cagttaaaaa taaaattcgt gcagcggcgc caatcctgaa ttctttatcc tcatataaag
     2101 acattggcaa tgtaaatccc aattttctcc cttcatatcg aggtgttaaa gcaaataaat
     2161 ttgatcgggc aattaagttt gtttcaaaat tgggtattct tcgtacaatc atcgcctgca
     2221 cataaaaatt gtgtggcaaa ggcttatcag cagttaatac aagagctgca ggtaaaaaca
     2281 tggaaaatgt gctggcatct tttgatgcat cactattgtc ataaataatt cgtgaaccgg
     2341 ttcggttaaa ttcatcaata tcaccaatag aatcgagcac cagattagca acaaccaacg
     2401 gctcttcctg taataaatga tacgaaccag aatttgattt aaatttaatt acacccaggt
     2461 caacaagaga agcaccaaat ttccaggtgt agtccaattt ttttgaatat ttaaatttag
     2521 attttttatg caatacaaaa tacccgccaa tatcaattcc gaaaccactt ccatttaatg
     2581 cataattatt tgggtcatac aataaatttg aacccagatt tctggtataa tcgaagtcag
     2641 catcaaaata tggagtagta gtatttactg tatctttcac ataattaaaa gggtcgttat
     2701 ttctaaatgc aaccgcttca aaacctgtta aatattttag attggcgcca aggtgtaaag
     2761 cataaacaga attactttct aaagtaaact caccattaat tccaacttca ccccaaatta
     2821 gcgcagctgc attaaattga ggcatatata atacggaatc aataggcaca tttaacacac
     2881 cggaaacaga catggtatct tttgtgctta atgcataacc ggcagttctt gcggtgaaaa
     2941 aagtaccaac tgcaaaatca tcaaatttta caaatgcgga tggtaattgt aataatacat
     3001 ttccatgtcc atataattta tgtggattat aaaaatcaac atcaattata ctatctgagg
     3061 tggtaacata ttttaaaagt gggtcatcta ttttgaggta attattatct tcatttaccc
     3121 caaatgccat catatttaca tcccatggta atttacctgc aattggagta gccgggttta
     3181 gcaacatacc atttgagccg gaataattat cagttcgcaa accaataaat tcctgcgcca
     3241 tggtattaga aaacaccaat actgtaaaac aaataattat cagcactcct cttggcataa
     3301 gccggaaatc atcaaatgaa taaacctgtt catgtgtgtg ttcaggtttg tgtgtgccga
     3361 cagttggttg gtataactgc cgaattaggt aataaacggc agcgtttgat aaatgttgct
     3421 gaaaaattat gacaaaaatt tgaactttcc gcaaaaagta agcatttttt ttgcagaagt
     3481 taattaatct accacttttc ccgggttaag aatgccattt gggtcgaata ttttgcgcag
     3541 atttctaaaa atattgagtt gtatttcgtt gaatgccaat ggcatatatt ttttttgcac
     3601 ccagccaata ccatgttctc cactaatggt gcctcctagt ttgataacca actgaaaaat
     3661 ttcggtaatc ccttggggca gccgcgactc ccattcttcg ttagtcaggg tcccttttaa
     3721 aatattgacg tgtaagttgc cgtcacctgc atggccataa caaactgatt gaaatccgta
     3781 tttatttcct atagctttaa caccttctaa caacactgac aattctgctc ttggaacaac
     3841 ggtatcttct tctttataag tagagtgttg tttaacagct tcgccaactt tgcgccgcat
     3901 tttccagagg tcgttttttt ctctttctgt ttcagcgaat aaaatttcat cacattcaaa
     3961 ttgttcaact atttgtgcaa ttgtttcaca atcctgcatt aatacatctt ggttattccc
     4021 atctacttca attaataaat gcgctttaat atggtctttt attgcaattt gaatatcggt
     4081 attaaattgt aaaacataat caatggcatc acgttccata aattccattg cacttggtgt
     4141 aatgccggct ctaaatattg ctgctactgc ttcacaggct ttagtagaat tagaaaatgg
     4201 caccagcatt aataagtttt tttgcggata aggaattaat cgcaacacta tttttgtaat
     4261 aattcccaat gtcccttcac tgccaacaaa caattgtgtc aaattataac cggttgcatt
     4321 ttttaataca ttagctcctg tccaaattat ttcaccggtt ggtaaaacaa cttccaaatt
     4381 cagcacataa tctttaacaa caccatattt aactgctttt ggtccgcctg aattttccgc
     4441 cacatttcca ccaataaagc aactgccttt actggccggg tcagggggat aaaacaaacc
     4501 tttttctatt acagcatttt gtaaaacttc ggttataaca cccggttcgg tggttacctg
     4561 caagttgcgt tcatctatat gaataatttt attaaacctt tccatgctca atgccacacc
     4621 accgcagacc ggcaatgcac caccgctaag tcctgttctg gcacccattg gcgtaaccgg
     4681 aaaatggtgg aggttggcta aaaccattat tgctgctatt tcttctgaag tgcccggttt
     4741 tactactact tcaggctggt aacgcaggtc ttcggtttcg tcatgcgcat acttttctag
     4801 gttttccccg tctagtaaaa cataatcatg accgacaatt tttgccagtt ggctaattac
     4861 atcagtgctt attggctgat acatggtgca aaggtattgg atttgcaatt ggtaacaatc
     4921 aacttataaa ccgccctgaa attgtcagat agatgtcagt acgtaggttt tccttgtaaa
     4981 gattatagga agtagctacc tttaataccc aaaactacga gaaatgaaaa aaaatattta
     5041 ccttttttta tttggtgtaa tcttactttc atcaaatacc ctttttgcgc aagcaaaaaa
     5101 atatccatta tttgaacatt tcacacaagc ttcttgtggt ccgtgcgcag cccaaaatcc
     5161 atttttccag gctgtttatg tggctaacaa aagtaatgtg catcatattg cttaccatac
     5221 ctcatggcca ggatttgacc caatgtataa tttcaatatg tcggagagtg atgaaatggt
     5281 tgattattat ggtgtatcag gtgtgcctga tatgattgtt aacggcgctg aatttggcag
     5341 tccagctggt gtaactcaag atgtagttaa caatttactt actgagtcaa gcccaatacg
     5401 tgtattagta actgaaacag ttgcaggttc tactgtaaat gttcatattg aagtgcaaac
     5461 cgtaggaaca attcctgcag ggacatataa aataaaagca gctgcggtag aaagaataat
     5521 cgagtatgca tctgcaccgg gaaccaatgg tgaattagat tttccaaatg tattccgtga
     5581 atcgttaacc ggaaccgatg gtgacgcaat tactcctgca gcaatcggtt cttcagtttc
     5641 atttgattat tcttatgatt tagcagctga atatgtagct gaaaacatgt atgctattgc
     5701 atgggttcaa aatgtttcgt caaaagatat tcttaactca ggtgcaagtg gtgatccaca
     5761 agtagaagtt gtaaatacaa ccacagcaac atttgctcaa ggaacaattg gcgctgccaa
     5821 tgcttttgaa ggcaacatta ttaatattgg tgatgctaat gcaaatattg aaattacatt
     5881 atcggcttct caacctgctg attggagttc aaatttcgta tataacggta caacatacac
     5941 tactaccgct tctgcatcaa ttaatgcatt gagtgaatta cctattaatt taaatgtgaa
     6001 tgttggagct acatccggca ttggagaata tacaattaca gttgaattcc ctgataatcc
     6061 ggaatttatg ccacaggtgt taagttatta tgtaatttca ggtgttacag atttaattgt
     6121 aaataatgaa gtaagttttg gtgacggcac accttatggt acttacaatt gggaatcatt
     6181 atatacagat gctttaaatg cggcaggtgt ttctacatac gcagctactt ctcatttcac
     6241 aatgaaaaaa ggtttcagta gcaattcact tgatgacatt gaaaatattt attacaacgt
     6301 tggttggaca tttcctggtt tagttgatga cgaaattaaa attgaacagt tgatggaatt
     6361 ccttgataat ggtggtaact tatttatttc aggacaagat attggatggg aagtaaatga
     6421 atatgctgcc ttttatcctc aagcagttga tttttatgaa aattatcttc atgcaactta
     6481 cgtaaatgat gcagcttcag gagctacaac tttaacagca gtagttgacg aaacctggta
     6541 tggcgatata gctgaaagtg aaataagcaa accgtacgga acaacttatt actaccctga
     6601 acaaattgaa gctaatggag cggaagcata cgacattttt acctacaatg caggtacaaa
     6661 agttggtggt gtgcgttctg aaacagccga ttataaaact gtttatttag gtattggaat
     6721 tgaaatgatt gaagacatta atgtgcgaaa tgccgttatg gaagctacct atttatactt
     6781 taaaggcaat attgatggta tagcttatga taatatcgta aaaggtttat taggtggagc
     6841 tcaacctaat ccagccgtta atgcaaccac aattacctta agcaatttgg ataaaaatta
     6901 tgttatttac gtaacagata taaccggtaa aattgtacta gaagataaca ttactgccgg
     6961 aactgatagt tataatttaa atacatcaga cctaagcagt ggtatgtatt tttactattt
     7021 aactgatggc caacaacgca cacaaacaga aaaactaaac gttgttaaat aaaacatcat
     7081 taattaaaaa agaatatgaa aaaattgatt ttactcccgg caattttatt tattgctact
     7141 acatcattcg ctcaaattat gttaatgcct tcagaagcag aaattgaagg tgatccaact
     7201 tcaattaatg aagtacacat tgaattccat aatatgggcg aaggtcaacc tatgacctgg
     7261 ataagaacag taaatgatat tccggaaggc tggaaaacat ctgtatgcga ttttaattta
     7321 tgctgggcag attttgctga tgaacctgat tatactttta gcgcacctgc cgatactatt
     7381 ggaactgtgt atgtaaaatt tgatgcacgc aatttccatg atggtgcatt tgacccaatt
     7441 ccgggttgcg gcaccgtgga ggttaatttt tatagcgtag ccgacagcgc aggatataat
     7501 gctttaggcg tatttcatgc tcgtttaggt gttgaagcgg aagattgtaa aacattaatt
     7561 gttacaccaa ttaatgataa taacttctta atatatccta acccggctaa aaacgttgta
     7621 aatgcagttg catcatttag cgctgatatt caacatgcag aaataattaa tactgcagga
     7681 attgttgttc gttcatacaa ttgggaaaca accagtggta aaatgacttt tgatttaaat
     7741 gatttacctg ccggaattta ttttatgcgt ttggtagatg taaataatgc aggtgtttat
     7801 accgaaaagg ttattataga ataacatttg ttaataaata aaagtaacac ctctgattgg
     7861 cttgccggtc aggggtgttt ttttgcctcg acggtttgtt tcgcgcggag aagggagttg
     7921 cggagagttg gtttaatgat aaattaataa taattctact gttttattac tacctgaaat
     7981 gcaagttatt aatttgaata ttcaataaaa tatttataca tttaactcag catgagcgca
     8041 caagttaatg atataaaata tcccgatatt ccggtttcgg aagtaatcga taatgataaa
     8101 tttaccattt acctgcgcaa cgatttaata gtttatgtgt taattaaaca aaacaatttt
     8161 tttgatgtgc ccgatgtaac cgaagtgctt gaaggtatta aaaaggttag caaaggcaat
     8221 atatttccac ttatagccgt gtacgaaacg aatattggct ttagtaaaaa tgccaaagac
     8281 atcgtagcca aacaccattt aaccaccgct gatgcattag ttaccctcga caactggcca
     8341 atcagggttt tggcaaattt ttatttgaaa gtaaataagc ccatacgtcc cactcgaatt
     8401 ttcggtgaag aaaccactgc aatcgaatgg ctgaaacttt atcgagagta gggattcagc
     8461 attttcttca cctgccggta gctgctacaa ttgctaaaaa gtctactgtt tttcaccttt
     8521 ttttcactat attattgtag ctgatttact accaaaacag ctctttatga aaaaatgtat
     8581 tgctgcacta atagctataa tgctatctgt agctggtttt agccaaaatg tttccatcaa
     8641 taatgatggg tcaacagccg acaactcggc aatgctggac ataaaatcta cttccaaagg
     8701 catattagtg ccacgcctta ctgccgcaca aaaactaggc atagctactc ctgccaccgg
     8761 cttattgatt taccagaccg atggaacaac cgggttttgg tattatgatg gcagtaattg
     8821 ggtcaatcta agtagc
//
LOCUS       DAORKW010000085         8832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_205_length_8832_cov_4.592344, whole genome shotgun sequence.
ACCESSION   DAORKW010000085 DAORKW010000000
VERSION     DAORKW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205_length_8832_cov_4.592344"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /gene="rplJ"
                     /locus_tag="PLB46_05110"
     CDS             complement(<1..189)
                     /gene="rplJ"
                     /locus_tag="PLB46_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRF75930.1"
                     /translation="MNKEQKSAVIEDLKEKVSQYDNFYLTDASAMTVEDVNKLRRLCF
                     ERGVKFEVVKNTLLRKAFD"
     gene            complement(215..913)
                     /gene="rplA"
                     /locus_tag="PLB46_05115"
     CDS             complement(215..913)
                     /gene="rplA"
                     /locus_tag="PLB46_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRF75931.1"
                     /translation="MKLTKNRKKVQALVDSNKLYSLREAAELVKKSTTCKFNASVDLH
                     VRLGIDPRKADQAIRGTTALPHGTGRDKKVLVLCTPDKEDEARKAGADYVGLDEFVDK
                     IVGGWIDMDVVIATPSVMPKIAKLGRVLGPRNLMPNPKVGTVTNEVGEAITEVKKGKV
                     AFKVDKYGIIHSSIGRVSFNPDQIAENSEELIKALAKLKPASAKGAYFKSITMASTMS
                     PGIAIDPKSVTEIH"
     gene            complement(936..1382)
                     /gene="rplK"
                     /locus_tag="PLB46_05120"
     CDS             complement(936..1382)
                     /gene="rplK"
                     /locus_tag="PLB46_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRF75932.1"
                     /translation="MAKEIQTYVKLQVKGGQANPAPPIGPALGSKGINIMEFCKQFNA
                     RTQDKMGKVLPVVITVYKDKSFEFIIKTPPAAILILEATKKQKGSAEPNRTKVGSISW
                     DQIKNIAQEKMPDLNCFTVESAMKMVAGTARSMGITVTGQAPWMNN"
     gene            complement(1384..1929)
                     /gene="nusG"
                     /locus_tag="PLB46_05125"
     CDS             complement(1384..1929)
                     /gene="nusG"
                     /locus_tag="PLB46_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRF75933.1"
                     /translation="MADKKWYVLRVISGKERKLRDYIEQHVKRLGWDTIVSQILVPTE
                     KIYKIKNGKKVIHEKNSLPGYILIEATEGKMTPEMMQTITGITSVIDFLGKEQPIALR
                     RNEVNRILGKMDEMSEEGTSMNEPFIIGETVKIIDGPFNEFLGNVDEVHEDKKKVKVI
                     VKIFGRKTPVELNFAQVEKVS"
     gene            complement(1949..2140)
                     /gene="secE"
                     /locus_tag="PLB46_05130"
     CDS             complement(1949..2140)
                     /gene="secE"
                     /locus_tag="PLB46_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRF75934.1"
                     /translation="MNKLRAFISEAYQELRFKVSWPTWPELQSSTQLVLVAFLIISVI
                     IFIMDLASKELLDLFYGLF"
     gene            complement(2156..2228)
                     /locus_tag="PLB46_05135"
     tRNA            complement(2156..2228)
                     /locus_tag="PLB46_05135"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2193..2195),aa:Trp,seq:cca)
     gene            complement(2271..3458)
                     /gene="tuf"
                     /locus_tag="PLB46_05140"
     CDS             complement(2271..3458)
                     /gene="tuf"
                     /locus_tag="PLB46_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRF75935.1"
                     /translation="MAKENFVRSKPHVNIGTIGHVDHGKTTLTSAITSVLSEKGLAQK
                     KDYDSIDAAPEEKERGITINTAHVEYETENRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPAIVVFMNKVDLVDDPELLDLVEMEVRDLLTF
                     YSFPGDTISIVKGSALKALEGEAGGKQAILDLMAAVDKDIPIPPRLTELPFLMPIEDV
                     FSITGRGTVATGRIERGIINSGDGVDIIGLTAEALKSTVTGVEMFRKILDRGEAGDNA
                     GLLLRGIEKDQLKRGMVICKPGSVTPHTEFKCEVYVLKKEEGGRHTPFFNKYRPQFYF
                     RTTDVTGEITLPEGVEMVMPGDNITITVKLIYPIAMEKNLRFAIREGGRTVGAGQVTE
                     ILK"
     gene            complement(3547..3619)
                     /locus_tag="PLB46_05145"
     tRNA            complement(3547..3619)
                     /locus_tag="PLB46_05145"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3584..3586),aa:Thr,seq:ggt)
     gene            complement(3734..3806)
                     /locus_tag="PLB46_05150"
     tRNA            complement(3734..3806)
                     /locus_tag="PLB46_05150"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3771..3773),aa:Gly,seq:tcc)
     gene            complement(3925..4007)
                     /locus_tag="PLB46_05155"
     tRNA            complement(3925..4007)
                     /locus_tag="PLB46_05155"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3971..3973),aa:Tyr,seq:gta)
     gene            complement(4017..4089)
                     /locus_tag="PLB46_05160"
     tRNA            complement(4017..4089)
                     /locus_tag="PLB46_05160"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4054..4056),aa:Thr,seq:tgt)
     gene            complement(4226..4528)
                     /locus_tag="PLB46_05165"
     CDS             complement(4226..4528)
                     /locus_tag="PLB46_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRF75936.1"
                     /translation="MDVKIHTIHFDADQQLLDFVNKKVGKIDKLDLKITDLQVSLKLD
                     AAIGGIHAKVAQIKANLPGKTIFSEEEAYTFEDAVEAAVDNALTQIKKHKEKQNIG"
     gene            complement(4557..5462)
                     /locus_tag="PLB46_05170"
     CDS             complement(4557..5462)
                     /locus_tag="PLB46_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRF75937.1"
                     /translation="MFLEGFYTYLQFEKRYSKNTLEAYSKDISQFQDFLIQEYQSDDP
                     ELLSVNHRQVRAWVIYLMNNNVTPKSVNRKISALKTYYRFLLKNNAIAKNPMAKVVSP
                     KIPKKLPLFVEKTGMEELFDVLDQVYPVETEVQRFVKQRDRLIVELLYSTGMRRAELL
                     GLSDNSFDRAQQQVKVLGKGNKERIIPVSKGLMLMADNYIAERKRIFGAGGPFIVTEG
                     GKPAYPKMVYNIVHQVLNHVTTLTRKSPHVLRHTFATHLSNNGAELNAIKELLGHASL
                     ASTQVYTHNTIDQLKKIHKLAHPKG"
     gene            complement(5515..5712)
                     /gene="rpsU"
                     /locus_tag="PLB46_05175"
     CDS             complement(5515..5712)
                     /gene="rpsU"
                     /locus_tag="PLB46_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRF75938.1"
                     /translation="MLIIDAREAESIDKALKNYKKKFEKAGIVKELRRRQSFSKPSID
                     RRQEIIKARYKQSFVTEANKG"
     gene            complement(5786..6925)
                     /locus_tag="PLB46_05180"
     CDS             complement(5786..6925)
                     /locus_tag="PLB46_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF75939.1"
                     /translation="MTVATSEHLSIIAESARDFAEKFIRPDMMEWDETQHFPLHVFHK
                     AGELGLMGVLVPHEYGGAGLGYIEYVNIIVEIASVCSSIGLSVAAHNSLCTGHILQFG
                     SEEQKHKYLPKLASATWIGAWGLTEPNTGSDAGNMQTTAVRDGDDWVLNGTKCWITHG
                     KSSDVMVVIARTGEPRTSSNCTAFIVERGTPGFSAGKKENKLGMRASETTEVIFDNCR
                     ISDAQRMGEVGDGFKQAMKVLDGGRISIAALSLGIAKGAYKAAIKYAKERQQFDQPIA
                     NFQGISFKLADMATRIEAAELLTIKAADMKNRGQKVTEISAMAKYYASEICVSVSNDA
                     VQVFGGYGYTKDFPVEKFYRDSKLCTIGEGTSEIQKLVIAREILK"
     gene            complement(6953..7483)
                     /locus_tag="PLB46_05185"
     CDS             complement(6953..7483)
                     /locus_tag="PLB46_05185"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRF75940.1"
                     /translation="MNIEQKIKKAIRDVADFPKEGVVFKDITPLLSDPVLSNDIAEAL
                     MERIKPLNLDAIVAIESRGFLYGFLLANKLNIPFIPVRKKGKLPGDTLEYTYDLEYGS
                     ATVEIHTKDIEPGWNVLIHDDLLATGGTAQAAAELLKMQGAHVAGFAFVVELTFLNGV
                     KKIKPYCDNIVSLATY"
     gene            complement(7480..8421)
                     /locus_tag="PLB46_05190"
     CDS             complement(7480..8421)
                     /locus_tag="PLB46_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF75941.1"
                     /translation="MQRKKKWRGYFTFSRRERNVGIYLMVMIVFITIAPFLLGFVQPK
                     PVSISKEIIANLDYHFVEVEEKKSANYSPFQYAPKNNSNSNFVDDKKSQITPTELSLF
                     YFNPNQITKQQWMQLGITPYVAERLVNYVAKGGKYKNKTDLLKTYGFTQSDLDRLSNY
                     ILLDSVITPIENKPVEKIVTINPIPTSLNTAGKEDLMVLGFSADNATRIIKFREGAGG
                     IYHADQLTTVFGIDAAVLEAAKPFLLADVSEITKININTATFEQLANHVYISDELAQA
                     IVNYRNTTGKFYTLTELRKVNGMYANLFEKLKPYLSI"
     gene            complement(8489..8632)
                     /locus_tag="PLB46_05195"
     CDS             complement(8489..8632)
                     /locus_tag="PLB46_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75942.1"
                     /translation="MSSIALTMMISTVSIVAVITGYFFWKVLKTPPREEPDSFSENDD
                     EKR"
     gene            complement(8629..>8832)
                     /locus_tag="PLB46_05200"
     CDS             complement(8629..>8832)
                     /locus_tag="PLB46_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75943.1"
                     /translation="IKQITNSGLKEQIAAAQTAGDQALVDTLTEKKLYINAARLLLLA
                     VFSGVAYLVYIAYRKRIKEGRFI"
BASE COUNT         2422 a         1848 c         1602 g         2960 t
ORIGIN      
        1 atcgaatgct ttgcgcaata aagtattttt cactacttcg aattttacgc ctctttcaaa
       61 gcataatctt cttaatttgt tcacgtcttc aacggtcatc gcagaagcgt ctgtcaagta
      121 aaagttgtcg tattgcgaca ccttttcttt aaggtcttca attacggccg atttttgttc
      181 tttgttcatc ttgtatgaat ttcgatgatt agcattaatg aatttcagtt acacttttag
      241 ggtcgattgc gataccaggg ctcatggtag aggccatagt aatgcttttg aaatacgcac
      301 cttttgcaga agccggtttt aatttagcca atgctttgat gagctcttca gagttttcgg
      361 caatttgatc aggattaaat gatacacgac cgattgaaga gtgaataatt ccgtatttgt
      421 ctaccttaaa ggcaaccttt ccttttttaa cttcggtgat ggcttcgcct acttcattgg
      481 taacagtacc aacttttggg tttggcataa ggtttcttgg acctaacaca cgaccgagtt
      541 ttgcaatttt aggcattact gatggtgttg caataacaac atccatgtct atccaaccgc
      601 cgacaatttt gtctacgaat tcatcaagac ctacgtaatc agcacctgct tttcttgctt
      661 cgtcttcctt gtcaggagta caaagcacga gaactttttt atctctacct gttccgtgag
      721 gtaatgcggt agttcctctg atggcctgat cggctttacg agggtcgata cccaatctca
      781 catggaggtc aacagatgca ttgaacttgc aggttgttga tttttttaca agttcagcag
      841 cttccctcaa cgaatatagt ttgttggagt caactaatgc ctgaaccttt tttctgttct
      901 ttgtaagttt cacttcttat aattttttag ttgacttaat tgttcatcca aggtgcttgt
      961 cccgtaacgg tgattcccat actgcgagca gttccggcaa ccattttcat ggcgctttct
     1021 acggtaaagc agttaagatc cggcattttt tcctgtgcaa tatttttaat ctgatcccag
     1081 gaaatagaac caacctttgt gcggttaggt tcagcagaac ctttttgttt tttggtagct
     1141 tcaaggataa ggatagcagc cggaggtgtt ttaatgataa attcgaacga tttatcttta
     1201 tacacagtaa taacaaccgg taatacttta cccattttat cctgggttct tgcattgaac
     1261 tgtttgcaga actccatgat gttgatacct ttagaaccta atgcaggacc aattggcggg
     1321 gcagggttcg cctgtcctcc tttaacctgc agttttacat acgtttgaat ttctttagcc
     1381 atatcagctt actttttcta cctgtgcgaa gtttaattcc accggcgttt ttctaccgaa
     1441 tatttttaca attactttaa cttttttctt gtcctcatgt acttcatcaa cattacctaa
     1501 gaactcgttg aatgggccgt caataatttt caccgtttca ccaataatga aaggttcatt
     1561 cattgaagta ccttcttcac tcatttcatc cattttaccc aaaatcctat tcacctcgtt
     1621 acggcgcagg gcaattggct gctcttttcc cagaaagtca ataacactgg taataccggt
     1681 tattgtttgc atcatttccg gggtcatttt cccttcagtt gcttcaatca ggatatatcc
     1741 cggcagactg tttttttcgt ggattacctt tttaccgttt ttaatcttat agattttttc
     1801 ggtaggaacc aggatttgag atacaatagt atcccatcca aggcgtttca catgctgctc
     1861 gatataatct ctcagcttgc gttcctttcc actgatcact cttaatacat accatttttt
     1921 gtctgccata agttatatga tctggtggtt aaaacaatcc atagaataag tccagcagtt
     1981 cctttgatgc caagtccata ataaatatta ttacagatat aatcaggaag gcaactaata
     2041 ccaattgggt actagactgc aattcaggcc atgttggcca actcacttta aaccggagtt
     2101 cctgatatgc ttcgcttata aatgctctta acttattcat tttctttttt tattgcacgg
     2161 gtggagaggc tcgaactccc agcctacggt tttggagacc gccactctac caattgagct
     2221 acacccgtat gggcggaaaa tccgctttac gggcgggttt tcctattatt ttattttaaa
     2281 atttcagtta cctgaccagc acctacggtt ctaccacctt cgcggatagc gaaacggagg
     2341 tttttctcca tagcgatcgg ataaatgagt tttactgtaa ttgtaatgtt atcaccaggc
     2401 attaccattt ctactccttc cggtaaggtg atttcacctg ttacgtcagt tgtacggaaa
     2461 tagaattgag ggcgatattt gttaaagaat ggagtgtgac gtccaccttc ttcttttttc
     2521 aatacgtaaa cttcgcattt gaattcagtg tgcggagtaa cgctacctgg tttacagata
     2581 accataccac gttttaactg atctttctca ataccacgga gtaataaacc agcgttgtca
     2641 ccagcttcac cacggtcaag aattttgcgg aacatctcaa cacctgttac agtagatttt
     2701 aatgcttcag cagttaaacc gataatatca acaccatcac ctgagttaat gatacctctt
     2761 tcgatacgac ctgtagcaac ggtaccacga ccggtgatag agaatacgtc ctcgattggc
     2821 atcaagaaag gtaattcagt cagacgcgga ggaattggaa tgtctttatc aaccgcagcc
     2881 attaaatcaa ggatagcttg tttaccacca gcttcacctt ctaatgcttt taatgcagaa
     2941 cctttaacga tagaaatagt atcaccaggg aaactataga aagtaagtaa atcacgaact
     3001 tccatttcaa caaggtctaa taattctgga tcgtcaacaa ggtcaacttt attcataaat
     3061 accacgatag caggaacacc tacctgacgt gctaaaagaa tgtgctcacg agtttgtggc
     3121 ataggaccat cagttgcagc aactacgaga attgcaccat ccatctgagc agcacccgta
     3181 accatgtttt tcacatagtc agcgtgtccc ggacagtcaa cgtgtgcata gtgacggttt
     3241 tccgtttcat attcaacgtg agctgtattg atggtgatac ctctttcttt ttcttcaggt
     3301 gcagcatcaa ttgaatcata atctttcttc tgagcaagac ctttttctga taatacagag
     3361 gtaattgctg aagtcagggt ggttttaccg tgatccacgt gaccgattgt accgatgttc
     3421 acgtgtggtt tcgaacgtac gaaattttct ttagccattt tataaaattt aaaaaattgt
     3481 attattaata tttactgttt actactactt tgttttgcag tccgattaca ctgcgattat
     3541 tttttgagcc aatgaccagg atcgaactgg tgacctcatc cttaccatgg atgcactcta
     3601 ccaactgagc tacattggct tttgttgcta ctttcactac tgcactttcc atgtgctcac
     3661 cttttaagca tttgccaaaa ggtttgcgga gcaccacccg ttaaggtaat attccgttta
     3721 tcgctattaa gcgagcggga gacgagactc gaacccgcga ctttcagctt ggaaggctga
     3781 cgctctacca actgagctac tcccgcttaa aattttaaat acagactgag gaaaatgacg
     3841 aagacagcaa tcctattaaa gcagaatgct ttgcaaggtt gttatattcg gcgtctttct
     3901 cagtttcaaa gaactgatgg atagtgggaa gaggtggatt cgaaccaccg aagtcgtaag
     3961 acaacagatt tacagtctgc cccatttggc cactctggta tcttccctcc gcctagagcc
     4021 aatggacgga ctcgaaccgc cgacctgctg attacaaatc agcagctcta ccaactgagc
     4081 tacattggcc tctatgtacc taatcgtatt tcaaagaaaa atcgaacgat tttcgcttga
     4141 ctttttacga aaatggatgc aaatatacga agtttttcaa ttgaatgtgt aaaacctcaa
     4201 aaaaaatact tggaattttt caggactagc caatattttg cttttccttg tgctttttaa
     4261 tctgggttaa agcgttatct actgcggctt ccaccgcatc ttcaaaggta taagcttctt
     4321 cctcagaaaa aattgttttt ccgggcaggt tggctttgat ttgtgcaact ttggcgtgta
     4381 tccctcctat tgcagcgtcc aacttcaaag acacttgcag gtcagttatt tttaaatcga
     4441 gtttgtcaat tttacccact ttcttgttta caaagtccaa caactgctga tcagcatcaa
     4501 aatggatggt gtgaattttt acgtccatgt cttttctttt gtgtaaatat atcatgctat
     4561 cctttaggat gcgctaattt gtgtattttt tttaattggt cgatggtatt atgcgtataa
     4621 acctgcgttg aagccagact cgcatggcca agcaactctt taatggcgtt gagttcagcg
     4681 ccgttattac tcaaatgcgt ggcgaaagtg tggcgcaaaa cgtgagggct tttacgtgtc
     4741 agtgttgtta cgtgatttaa aacctggtgc actatattat ataccatttt cggataagcc
     4801 ggtttacccc cttcggttac aataaaaggg ccgccggcgc caaatattct tttccgctct
     4861 gctatataat tgtctgccat cagcatcaat cctttactaa caggtataat tcgctctttg
     4921 ttcccctttc cgagcacttt tacctgttgc tgggccctgt caaacgaatt gtcgctaagt
     4981 cccagcaact ccgccctacg cattcctgtg ctgtataata actcaacgat aagcctgtcg
     5041 cgctgtttga caaatcgctg cacctcggtt tctacagggt aaacctggtc gaggacgtcg
     5101 aaaagctctt ccatgccggt tttttcgaca aaaagtggca gttttttggg aattttgggt
     5161 gaaaccacct tggccattgg atttttagca atggcgttgt tttttaataa gaaacgatag
     5221 taagttttaa gggcggaaat tttcctgttt acgcttttgg gagtgacgtt attattcatc
     5281 aaataaataa cccaagccct tacctgacga tggtttacac tgagcaactc cgggtcgtcg
     5341 gactggtatt cctgaatcaa aaaatcctga aattgggaaa tatctttgct ataggcttcg
     5401 agggtatttt tgctataccg tttctcgaac tgtaggtatg tgtaaaagcc ttccaaaaac
     5461 ataaacgaat ttgaaacgtt agcaaaccag cccttacact aaataattaa tgtattagcc
     5521 tttgttagcc tcagttacaa atgactgttt gtaacgagcc ttgataattt cttgtctgcg
     5581 atcaattgat ggcttagaaa aagattgtct tctacgtaat tcttttacga taccagcttt
     5641 ctcaaatttc tttttgtaat ttttgagtgc tttgtcaatt gattcagctt ctcttgcgtc
     5701 gatgattaac attaattttt attttgggac tgcaaaaata gggtttgtta tttaaaatac
     5761 ctaatacgga aaaaaattct gttggttact taagaatttc gcgggcaata acgagttttt
     5821 gtatctcaga agtgccttct cctatggtac aaagtttaga atcacggtaa aatttttcta
     5881 cagggaagtc tttggtatag ccatatccgc caaaaacttg aaccgcatca ttggatacac
     5941 tcacacagat ttcggaagcg taatatttcg ccattgctga tatttcggtc accttttggc
     6001 ctcggttttt catgtcggct gccttaattg taagtaattc ggctgcttcg atacgggtgg
     6061 ccatatcagc taatttgaat gaaatacctt ggaaattggc aataggctgg tcgaactgct
     6121 gacgttcttt agcatattta attgctgctt tataagcgcc ttttgctata cctaaactca
     6181 atgcggcaat agaaatacga ccaccatcga gcactttcat agcttgttta aagccgtcgc
     6241 caacttctcc catacgttgt gcatcagaaa tgcggcaatt atcaaaaatt acttctgtgg
     6301 tttcgctggc ccgcatgcct aatttatttt cttttttacc tgcgctaaaa ccaggagtgc
     6361 cgcgttctac gataaatgct gtgcaattac ttgaagtgcg cggttcgcct gttctggcaa
     6421 tcactaccat tacatcgctc gatttgccat gtgtaatcca gcattttgtg ccatttaaaa
     6481 cccaatcatc gccgtcgcga actgcagtgg tttgcatatt accggcgtcg ctacctgtgt
     6541 taggttcggt taaaccccaa gcacctatcc aggttgcgga agctaatttt ggtaaatatt
     6601 tgtgtttttg ttcttctgag ccaaattgta aaatatgacc agtacataat gaattatgtg
     6661 ctgctacaga caaacctatt gaactacaaa cagaggctat ttcaactatg atatttacat
     6721 attcgatata tcctaaacct gcaccaccat attcatgagg aacgagtaca cccattaaac
     6781 ccaattcgcc agctttgtga aatacatgga gcgggaaatg ttgagtttcg tcccattcca
     6841 tcatatcagg gcgaataaat ttttctgcga aatcgcgggc agactcggca attattgaca
     6901 aatgttcaga agtagctaca gtcatatttt tatcttcagg actgcaaaag tatcaatatg
     6961 tagccaatga aacaatattg tcgcaataag gttttatttt tttaacacca tttaaaaatg
     7021 tgagttctac cacaaacgca aatcctgcaa catgtgcacc ctgcattttc aacaattcgg
     7081 ctgcggcttg agcggtacct cctgtagcta ataaatcgtc atgaattaac acattccatc
     7141 ccggctcaat atccttagta tgaatctcaa ctgtggctga gccatattct aagtcgtaag
     7201 tatattctaa cgtatctccg ggtaatttcc cttttttgcg taccggtatg aatggaatat
     7261 ttaatttatt tgccaataaa aatccataca aaaatcctct gctttcgatg gcaactattg
     7321 catctaagtt taatggttta attctttcca ttaacgcttc agcaatatca ttagataaaa
     7381 ccgggtcaga taataatggt gtaatatctt taaatactac tccttctttt ggaaaatcag
     7441 ctacatctct gatagctttt tttatttttt gttcaatatt catatgctta aataaggttt
     7501 caatttttcg aaaagattcg catacatacc atttactttt ctcaattcag ttaatgtgta
     7561 aaatttgccg gttgtatttc tataatttac gatcgcttgc gctaattcat ctgaaatata
     7621 cacatgattg gcaagttgtt caaatgttgc tgtatttata tttattttag taatctcgct
     7681 aacatctgcc aacaaaaatg gttttgctgc ttccagcact gcggcgtcaa ttccaaaaac
     7741 tgtcgttaat tggtcagcat gataaattcc accagcacct tcgcgaaatt ttataatgcg
     7801 cgttgcatta tctgcagaaa atcctaacac cattaaatcc tctttaccgg ctgtatttaa
     7861 actagtagga attgggttta tggtaacaat tttttctacc ggtttatttt caataggtgt
     7921 aattacagaa tctaataaaa tataattact taatctgtct aaatcactct gtgtaaatcc
     7981 gtaggttttt agtaaatcag ttttgttttt atattttcca cctttagcaa cataatttac
     8041 caatcgttcg gcaacataag gagttatgcc taattgcatc cattgttgtt tagtaatttg
     8101 attgggatta aagtaaaata atgataattc agttggagta atttgacttt ttttgtcatc
     8161 aacaaaatta ctattcgaat tatttttggg tgcgtattga aaaggcgaat aattggcgga
     8221 ttttttttct tccacttcaa caaaatgata atccaaattg gcaattattt ctttacttat
     8281 tgataccggt ttcggctgca caaaaccaag caggaatggt gctatcgtga taaatacaat
     8341 catcaccatt aaataaattc ccacatttcg ctctctcctt gagaaagtaa aatatccacg
     8401 ccacttcttt ttcctttgca tgcctaaaaa taaacaaaaa ggagataatc aattgactat
     8461 ctcctgtagt atgttcagat gataataatc atcttttttc atcatcattt tccgaaaagg
     8521 aatccggttc ttctcttggt ggtgttttta atactttcca gaaaaaatat cctgtaatta
     8581 ctgctacaat agaaacagta ctaatcatca ttgttaatgc gatagaactc atataaacct
     8641 cccttctttt atgcgtttac gatatgcaat ataaaccaaa tatgctacgc cgctaaatac
     8701 ggcaagtaat agtaatctgg ctgcattaat atataatttc ttttcagtta aagtatcaac
     8761 caatgcctgg tcgcctgctg tttgtgctgc agcaatttgt tcctttaatc cggaattagt
     8821 tatctgtttg at
//
LOCUS       DAORKW010000086         8807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_207_length_8807_cov_5.421504, whole genome shotgun sequence.
ACCESSION   DAORKW010000086 DAORKW010000000
VERSION     DAORKW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207_length_8807_cov_5.421504"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="PLB46_05205"
     CDS             complement(<1..1248)
                     /locus_tag="PLB46_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRF75944.1"
                     /translation="MATNKKQQLFSGSDYAIFRRLMSYAGPFKRQFYISTILAITLSL
                     LTPLRPFLVGVTVDTYILGNNYNGLVLITLISLLVLFAESICKYFFLFLTNWLGQHVV
                     KNLRVKVFSHLIGLRLSYYNNTPVGISTTRTINDLETINSVFTEGAIQIIADLLTIVF
                     VITFMFITNWQLTLLSLISLPMIVYATYVFKEGIKSTFQEVRTAVARLNSFLQERITG
                     MKVVQIFNAEDQEYENFKLINKDHRGAQVRSVWYYSIFFPVVEIIIAFATALMVWRGA
                     HSVLQGHATLGDINAFILYLSILFRPLRMLADKFNTLQMGVVSSDRIFKILDTREHIE
                     NTGTYAPDKVAGKIDFKHVWFAYKDKDWVLRDVSFQIKPGETLAIVGATGAGKTSIIN
                     ILNRFYEIQQGDILIDGHSVKSYE"
     gene            complement(1323..2513)
                     /locus_tag="PLB46_05210"
     CDS             complement(1323..2513)
                     /locus_tag="PLB46_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRF75945.1"
                     /translation="MEHIIDKIKSLAIENFEEIRECRRHLHRHPELSFEEFATSRYIQ
                     QQLTKFGVSFKADIATTGVVAIIEGNNPGKKTIALRADIDALPITETNTHDYVSTNHG
                     VMHACGHDAHTASLLGVAKILSALKNEFEGTVKLIFQPSEEKLPGGAAVMIEEGVLEN
                     PKVESIFAQHVFTPFEVGTVAFCFGNMLASTDEIYITVKGKGGHGAYPHETKDPVVMA
                     AQMLVALQQVVSRNMSPFQPTVLTFGKVIANGATNVIPDEVNLAGTFRAMDEKVREQA
                     HQLIHQIATKTVEALGGTAEINIVKGYPVLVNDDAVTKRSYDRAVQYLGADKVKITTP
                     RMGAEDFAFYSQKVPACFYRLGTGNPEKGITSNIHTPTFNIDEDALKIGMGLMAYHAI
                     SELS"
     gene            complement(2516..2899)
                     /locus_tag="PLB46_05215"
     CDS             complement(2516..2899)
                     /locus_tag="PLB46_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRF75946.1"
                     /translation="MKFILTGLLFLVVAAGYCQTGTVVINDQAGATQLIEQHVLFNKE
                     HGELPGYRIQVMATSSLVTAKEAKAGFLKQYDDYRATIVFEAPNYKLRIGNYTNRFDA
                     NRDLQLLLEAYPNALIVKDLISITE"
     gene            complement(2987..3643)
                     /gene="deoC"
                     /locus_tag="PLB46_05220"
     CDS             complement(2987..3643)
                     /gene="deoC"
                     /locus_tag="PLB46_05220"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRF75947.1"
                     /translation="MSSNILLNKYIEHTILRPDTTILEVEQICHEALKYQFAGVCVPS
                     YFLESAVELLTDTGINIVTVIGFPYGYNSLMSKFEEAEDAINRGADHLDMVINIAAVK
                     NNDWETVENEIDTINRLVKRENKIFKLIAETGLMSEIELELICKIANDLKIDYLKTST
                     GVNGAGANVEVVTAMREMLDNGIKIKASGGIRTRAAALELIEAGADRIGASKSVEMMG
                     "
     gene            complement(3697..7011)
                     /gene="secA"
                     /locus_tag="PLB46_05225"
     CDS             complement(3697..7011)
                     /gene="secA"
                     /locus_tag="PLB46_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRF75948.1"
                     /translation="MSLISNVLSKLFGGNKYEKDVKAITPIIEQVKAAYAQLSKLSND
                     QLRNKTAEFKSRIAEHLKQIDAEIAVLRAEADDPNLLDVDRKDDIYKDIDKLRKDRDK
                     KIEEVLLMILPEAFAVMKETARRFTENEKVISTATAMDRDLSVNHDFVQIEGGNAIYS
                     NEWTAAGVPIKWNMVHYDVQLIGGYVLHSGKIAEMATGEGKTLVATLPAYLNALAGEG
                     IHIVTVNNYLARRDAEWNAPIFNFLGLRVDCIDLYQPNSPERRNAYLADITYGTNNEF
                     GFDYLRDNMVRSVEEKVQRKHHFAMVDEVDSVLIDDARTPLIISGPIPKGGDAEVYNQ
                     LKPRIHKLIEAQKKVFTQNLNDAKKMIEAGRNKEGGVHLVKAYRSLPKNNALIKLLSE
                     EGVRKLLMDTEGIYIAEHNKRMPEIDQDLYFVIDEKSNSVDLTDKGIELITEGGEDTN
                     FFIIPDVGVQIAEIERNIFDKEEKLAAKDKLMQDFAIKSDRIHSVQQLLKAYTLFEKD
                     VEYVVMDSKVKIVDEQTGRILDGRRYSDGLHQAIEAKENVKVEALTQTYATITLQNYF
                     RMFHKLCGMTGTAETEAGEFWDIYKLDVSAIPTNKPIVRKDLEDYVYKTRKEKYLAVI
                     DRIQQLVKDGRPVLVGTTSVEVSELLSRMLQLKGIKHSVLNAKQHQREAEIVAEAGKA
                     GSVTIATNMAGRGTDIKLGIGVVDAGGLAIIGTERHDSRRVDRQLRGRAGRQGDPGSS
                     EFFVSLEDNLMRLFGSERIAKVMDTMGYKEGEVIQHRMITKSIERAQKKVEENNFGMR
                     KRLLEYDDVMNNQRSLVYQKRNHALSGERLTIDLNNTIFDLCEELVNTHHAAKDYENF
                     TLDVIRYFSMEPEITQRDFENQKPDVITRNLYNNALAYYKRKSQVLLNKVYPLVQDLH
                     ASRGETLENVIVPFTDGRKGLNVMVNLTKAIETNGLEINYSIERMISLIVIDDHWKEH
                     LREMDDLKQSVQGAVYEQKDPLLIYKFEAFEMFKKMLVELNKEIVSFLFKGYVPIRSA
                     EEVRSAVPQKRTDMSQLKETREDAAGPADGTPRTAAQPERKPVEPIRVEKKVGRNDAC
                     PCGSGKKYKNCHGKEE"
     gene            7138..>8807
                     /locus_tag="PLB46_05230"
     CDS             7138..>8807
                     /locus_tag="PLB46_05230"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRF75949.1"
                     /translation="MHECSIVLALHRISTMGYAIKDIVAILGNRGQIVSPEAQIEHLL
                     TDSRNITFAGQSLFIAISGKQHDGHQYIMDAYNAGVRSFLVEKMPEPPLSDANFIVTP
                     NSLLALQQLAAHHRSYYHLPVIGITGSNGKTIVKEWIYHLLKIDFDIVRSPKSYNSQL
                     GVPLSIWAIDKQHELGIFEAGISLPDEMEVLEKIIQPNIGIFTNITNAHNEGFSDEDQ
                     KVSEKLKLFAQADIIIYRKDYTSIDYALKNNNSVLSWSTDATKNPDILISDVETLIDK
                     TLFTIHFLQQHFHFAIPFADEASFENAVHALIASLFIANNTEQLNDEMLNRITIQAQE
                     LPVISMRLELKKGNNNCLLINDAYSADIASLDIALRFMHHHADDLTKTIIISDFDESG
                     ESDEQLYQKVAALLKENGIKNVYAIGAALEKNQIIFNDFNSWFFNNTQDFLKQINSLS
                     FEQQIILIKGARRFQLEKIGNQLALKTHGTVLRINMNNLAHNLNIYRAMLNPGVKTMA
                     MVKAFSYGSGQAEIARLLANQRVDYLAVAYADEGADLRRQGIKLPIMV"
BASE COUNT         2696 a         1778 c         1464 g         2869 t
ORIGIN      
        1 ctcatatgat tttacagaat gtccatctat taaaatatca ccttgctgaa tttcgtaaaa
       61 tcggttaagg atattaataa ttgatgtttt acctgctccg gttgcgccga caattgccaa
      121 ggtttcaccg ggtttaattt gaaaactaac atcacgcaat acccaatctt tatctttata
      181 tgcgaaccat acatgtttaa aatcaatttt accggctact ttatccggtg catacgttcc
      241 ggtattttcg atatgttcgc gagtatccag tattttaaaa attctgtcgc ttgataccac
      301 tcccatttgc aaagtattaa atttatctgc caacatacgc agtggcctga ataaaatact
      361 caggtataat ataaatgcat ttatatcacc caatgtggca tgaccttgta aaacactatg
      421 cgccccacgc cataccatga gtgctgtagc aaaggcaata attatttcta caaccggaaa
      481 aaagatggaa taataccaaa cagacctaac ctgcgctcct cgatggtctt tgttaatcaa
      541 tttaaaattt tcgtattcct ggtcttcagc attaaatatt tgtactactt tcatacccgt
      601 aatacgttcc tgcagaaaag aatttaatct tgctaccgca gttcgcactt cctgaaaggt
      661 tgacttaata ccttctttaa agacatacgt ggcataaaca atcatgggca aactaatcaa
      721 actcaaaagc gtcaattgcc agttcgtaat aaacataaaa gtgattacaa aaacaattgt
      781 cagcaaatcg gcaattattt gtatggcacc ttcagtaaat acagagttga ttgtttcgag
      841 gtcgttaata gttcttgttg ttgaaatacc aaccggtgta ttattgtaat agcttaaccg
      901 caaaccaatc aaatgactaa aaacttttac gcgcaagttt ttcactacat gttggcccaa
      961 ccaattggta aggaataaaa aaaagtattt acatattgat tccgcaaata acaccaaaag
     1021 tgaaatcaag gttattaata caaggccgtt gtaattatta cccaaaatat aggtatcaac
     1081 tgtgacccct accaaaaatg gcctaagcgg tgttaacaac gaaagggtta ttgctaaaat
     1141 ggtagaaata taaaactgcc gcttaaatgg accggcataa ctcattagcc ttctgaaaat
     1201 cgcataatca gagccggaaa aaagttgctg tttcttattg gttgccaagc agaattatta
     1261 caaaaactaa agccgcaaag ttcagtcttt tcaagtaaat aatccactaa ttgagaaaga
     1321 aatcaggata attcgctaat tgcgtggtat gccatcaacc ccatacctat ttttaatgca
     1381 tcttcatcga tattaaatgt aggcgtatga atattagacg ttataccctt ttcaggattt
     1441 cctgttccaa gcctgtaaaa gcaagccgga actttttgcg aataaaatgc aaaatcttca
     1501 gcgcccattc taggtgtagt gattttcact ttatctgctc ctaaatattg aaccgctcta
     1561 tcgtaactac gtttagtaac tgcatcatca tttaccagaa caggatatcc tttaacaata
     1621 tttatttctg ctgtgccacc aagtgcttca actgtttttg ttgcaatttg atgtataagt
     1681 tgatgagcct gttcgcgcac tttttcatcc attgccctga atgttcccgc taaattcact
     1741 tcatcgggaa taacattagt agcgccgttg gcaattactt ttccaaaggt taatacagtt
     1801 ggttgaaatg gcgacatatt acggctaacc acttgttgta aagctaccag catttgtgca
     1861 gccataacaa ccgggtcttt ggtttcatgc gggtaggctc catgaccgcc ttttcctttt
     1921 acggtaatat aaatttcgtc ggtactcgcg agcatattgc caaaacaaaa tgcaacggtt
     1981 cctacttcaa atggagtaaa tacatgttgc gcaaaaatac tttctacttt aggattttcc
     2041 agcacacctt cttcaatcat aactgcagca ccacccggta atttttcttc tgaaggttga
     2101 aaaattaatt tcactgttcc ttcgaattca ttttttaaag cggataaaat tttagctaca
     2161 cctaataaag aagcagtatg ggcatcgtgg ccacaagcat gcattacacc atgattagtt
     2221 gaaacataat catgtgtgtt tgtttccgta atcggcaagg catcaatatc ggcgcgaaga
     2281 gcaattgttt tttttccggg attatttcct tctattatcg ccacaactcc ggtagttgca
     2341 atatcagctt taaagcttac accaaatttt gtcagttgtt gctgaatata tcgcgaagta
     2401 gcaaattctt caaatgataa ttcaggatga cgatgaagat gtcggcgaca ctctcgaatt
     2461 tcttcgaaat tttcaatcgc taacgattta attttatcaa taatgtgctc cattattatt
     2521 ctgttatgct aatcaaatct ttaacgatta gcgcattagg gtaagcttct aataaaagtt
     2581 gcaagtcgcg gttggcatca aaccgatttg tgtaatttcc aattcgcaac ttataatttg
     2641 gtgcttcaaa tacgattgta gcccgataat catcatactg ttttaaaaat ccggctttag
     2701 cttcctttgc cgtaactaaa ctacttgttg ccataacctg aatgcgataa ccggggagtt
     2761 cgccatgttc tttattaaat aatacgtgtt gctcaatgag ttgagttgca cccgcctggt
     2821 cattaataac aaccgtgcct gtttggcagt agcccgcagc aacaactaaa aacaacaatc
     2881 cggttaaaat gaatttcata cgattaggtg gcactaaaat aataataata aacgattagc
     2941 aggggctacc aaatatatag caggccaaaa aatatcattt ccgggcttat cccatcattt
     3001 caacactttt ggaagcgcca atacggtctg cacctgcttc tatcagttct aaggcagctg
     3061 ctcgggttct gattccaccg gatgctttta ttttaatacc attatcgagc atttcgcgca
     3121 tagcagtaac tacttccaca ttagcccctg ccccattaac acctgttgaa gttttgagat
     3181 aatcgatttt taaatcattg gcgattttac aaatcaactc caattcaatt tctgacatta
     3241 aaccggtttc ggcaattaat ttaaaaattt tattttccct ttttaccaaa cggttaatgg
     3301 tatcaatttc attttctaca gtttcccagt cgttattttt tactgctgca atattgatta
     3361 ccatatctaa atggtctgcg ccgcgattaa ttgcatcctc agcctcttca aatttgctca
     3421 tcagcgaatt gtagccataa ggaaaaccaa ttacggttac aatattaata ccggtatcag
     3481 ttaataattc caccgctgat tctaaaaaat aagatggaac acaaactccg gcaaattgat
     3541 attttaatgc ttcgtgacaa atttgttcta cttcaaggat agtagtatcg ggtctgagta
     3601 tagtatgctc aatatattta ttaagcagta tgtttgatga catatagata taaggaatta
     3661 aaatgtcccg ataaaaatta ccgggactta aataatttat tcttctttac cgtgacagtt
     3721 tttatatttt tttcctgaac cacaaggaca tgcatcgttg cgaccaactt tcttttcaac
     3781 gcgaattggt tcaacaggtt tgcgttcagg ttgcgcggct gttcttggtg ttccgtctgc
     3841 tggacctgct gcatcttcac gagtttcttt gagctgactc atgtcggtgc gtttttgtgg
     3901 aacagctgac ctcacttctt ctgcgctgcg aataggcacg taacctttaa ataggaatga
     3961 aacaatttct ttatttaatt caacaagcat ttttttaaac atttcgaatg cttcgaattt
     4021 ataaattaac agtggatctt tttgctcata tactgctccc tgaaccgatt gtttcaaatc
     4081 gtccatttca cgtaagtgtt ctttccaatg atcgtcgata acaattaaac taatcattct
     4141 ttcaattgaa taattaattt ccaaaccgtt tgtttctatt gctttggtta aattaaccat
     4201 tacattcaac cctttacgac catcagtaaa cggaactata acgttttcta aagtttcacc
     4261 tcttgaggca tgtaaatctt gcacgagtgg atacacttta tttaacagca cttgcgactt
     4321 acgtttataa taagctaatg cgttattata caaatttctt gtaataacat caggcttttg
     4381 gttttcgaaa tcgcgttgag ttatttccgg ctccatactg aagtatcgaa ttacatccag
     4441 tgtgaaattt tcgtaatctt tagctgcgtg atgtgtatta accagctctt cacacaaatc
     4501 aaatatggta ttatttaaat cgattgttaa acgttctcca gataatgcat gattacgttt
     4561 ttggtaaact aatgaacgtt ggttattcat aacgtcatcg tattcgagta atcgtttacg
     4621 cataccgaag ttattttctt caactttctt ttgtgcgcgt tcgatagatt tggtaatcat
     4681 acgatgttga attacttcac cttctttgta acccatggta tccattactt tggcaatacg
     4741 ttcactacca aacaaacgca tgaggttatc ttcaagcgac acaaaaaatt cgctggatcc
     4801 aggatctccc tggcgaccgg cacgaccgcg taactgacga tcgacacgac gcgaatcgtg
     4861 acgttcagta ccaataattg ccaaaccacc ggcatcaaca acaccaatac ctaatttaat
     4921 atcggtacca cgaccggcca tattggttgc aatagttaca cttcctgctt tacctgcttc
     4981 cgcaacaatt tcagcttcgc gctgatgctg tttcgcattt aatacactgt gtttaattcc
     5041 tttaagttgc agcattcggc ttagcaattc actcacctca actgaagttg taccaaccaa
     5101 aaccgggcga ccatctttta cgagttgctg aatgcggtca ataactgcaa ggtatttttc
     5161 tttacgtgtt ttataaacgt aatcttctaa atcttttcta acaattggtt tgttggtcgg
     5221 aattgccgaa acatccaatt tataaatatc ccaaaactca cccgcttccg tttctgcagt
     5281 acctgtcata ccgcataatt tatgaaacat acggaaatag ttctgtaaag taattgtagc
     5341 atacgtttgt gttaatgctt ctacttttac attttctttt gcttcaattg cctgatgtaa
     5401 accatcacta taacgacgac catctaatat acgtcctgtt tgctcatcaa caattttcac
     5461 tttactatcc attaccacat actctacatc tttttcaaat aaagtatatg cttttaataa
     5521 ttgttgaaca gagtgaatac ggtcgctttt aatagcgaaa tcttgcatca atttatcttt
     5581 agctgcaagt ttttcttctt tatcaaaaat attgcgttca atttctgcaa tttgtacacc
     5641 aacgtcagga ataataaaga aattagtatc ctctccgcct tcagtaatta actcaatacc
     5701 tttatcagtt aaatcaacgc tattactttt ttcatcaata acgaaatata aatcctggtc
     5761 aatttcaggc atacgtttat tgtgttcagc gatataaata ccttctgtat ccatcaataa
     5821 tttacgaaca ccttcttcac ttaataattt gatgagtgca ttgttttttg gcaatgaacg
     5881 atatgcttta accaaatgaa ctccgccttc tttatttctt ccggcttcaa tcattttttt
     5941 cgcatcattc aaattttgcg taaacacttt cttttgtgcc tcaattaatt tatgaattcg
     6001 tggttttaac tgattataca cttcagcatc tccaccctta ggaattggac ctgaaataat
     6061 taatggtgtt ctggcatcat caattaaaac tgagtcaacc tcatccacca ttgcgaaatg
     6121 atgtttacgt tgaacttttt cttctactga gcgcaccatg ttatcgcgca aatagtcaaa
     6181 gccaaattcg ttatttgtgc cgtaggtaat gtctgctaaa taagcatttc ttctttccgg
     6241 tgaattaggc tgatacaaat cgatacaatc tactcttaag ccaaggaaat taaatattgg
     6301 cgcattccat tccgcatcac gacgagcgag gtaattattt acagttacaa tgtgaatacc
     6361 ttcaccagct agtgcattta aatatgcggg caaagttgcc acgagggttt taccttcacc
     6421 ggtagccatt tcggcaattt ttccggagtg taatacatat ccacctatca gctgcacatc
     6481 ataatgtacc atattccatt taattggcac acctgctgca gtccattcgt tgctataaat
     6541 tgcattacca ccttcaatct gaacaaaatc gtgatttaca gataaatcgc gatccatagc
     6601 agttgctgta gaaataactt tttcattttc ggtaaatctg cgtgcagttt ctttcatcac
     6661 cgcaaaagct tcgggaagta tcatgagtaa cacttcttca atttttttat cacggtcttt
     6721 ccgcagctta tcaatatctt tatagatatc gtccttacgg tcaacatcca ataagttcgg
     6781 gtcatctgcc tcggctctta aaacagcaat ttcagcatca atttgcttta agtgttcagc
     6841 aatacgactt ttaaactctg cagttttatt acgtagttga tcgttagaaa gctttgaaag
     6901 ctgcgcatat gctgctttta cctgctcaat aataggcgta atcgccttaa catctttttc
     6961 gtacttattg cctccaaata actttgaaag gacgttggat attaaactca tatatatatt
     7021 tactcaattg aaagaatgca gttgtcaaaa gtcgttcctg catttgccgc aaagataagg
     7081 attgtgttgt gtaggggtat attttaacac atcatttaca gctaaaaccc cgaaaacatg
     7141 catgaatgtt caattgtatt agctttacac cgaatttcaa ctatgggtta cgcgattaaa
     7201 gatattgttg ccatactcgg aaaccgggga caaattgtca gcccggaggc ccaaattgag
     7261 catttattga cagattcccg caatataacg ttcgccggcc aatcgctgtt tattgccatt
     7321 tcgggtaaac aacatgacgg gcaccagtat ataatggatg cctataatgc cggtgtgcgt
     7381 tcgtttttgg ttgaaaaaat gcccgaacca cctctttcgg atgccaattt tatcgtgact
     7441 ccaaatagtt tgcttgcttt acaacaatta gctgcccatc acagaagcta ctatcacctg
     7501 ccggttattg ggattacagg tagcaatggc aaaaccattg tgaaggaatg gatttatcat
     7561 ttattaaaaa tcgatttcga cattgttagg agcccaaaaa gttataactc acaacttgga
     7621 gttccattaa gcatttgggc tattgacaaa caacatgagt tgggtatttt tgaagcaggc
     7681 atttcattac ctgatgagat ggaagtattg gaaaaaatta ttcaacctaa tattggcatt
     7741 ttcacgaata ttactaacgc acataatgaa ggattttctg atgaagacca aaaagtgtca
     7801 gaaaaattaa aattatttgc ccaagctgat attatcattt accgcaaaga ttatacatca
     7861 attgactacg cattaaaaaa taataattca gtattaagtt ggagcacaga tgcaacaaaa
     7921 aatcctgata tattaattag tgatgttgaa acattaattg ataaaacttt attcaccatt
     7981 cattttttac aacaacattt tcacttcgct attccttttg cagatgaagc ttcatttgaa
     8041 aatgcggtgc atgcattaat agcctcgttg tttatcgcca acaacactga gcagttgaac
     8101 gatgaaatgc ttaaccggat aacaatacag gctcaagaac tacctgttat aagcatgcgg
     8161 cttgaattaa aaaaaggcaa taacaactgt ttattaatta atgatgctta cagtgccgat
     8221 attgcctcct tagatattgc attacgattt atgcatcacc atgctgatga tttgacaaaa
     8281 acgataatca tcagcgattt tgatgaaagt ggtgaaagtg atgaacaatt atatcagaaa
     8341 gttgcagcac tattaaaaga aaatgggatt aaaaatgtgt acgctattgg tgcagctttg
     8401 gaaaaaaatc aaattatttt taacgatttc aatagttggt tctttaacaa cacacaagat
     8461 tttttaaaac aaataaacag cttatcattt gaacagcaaa taatattaat taaaggcgca
     8521 cgaagatttc aattagaaaa aatcggcaat cagcttgcat taaaaacgca tggcactgta
     8581 ttaaggatta acatgaataa tcttgcgcat aatttaaata tttacagagc aatgctgaac
     8641 ccgggtgtca aaactatggc catggtaaag gcatttagtt atggcagtgg acaagcggaa
     8701 atagcaaggc ttctggccaa tcagcgtgtc gattatctgg cagttgctta tgctgacgaa
     8761 ggtgctgatt tacgtcgtca aggcattaaa ttacctatta tggtaat
//
LOCUS       DAORKW010000087         8719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_210_length_8719_cov_6.296745, whole genome shotgun sequence.
ACCESSION   DAORKW010000087 DAORKW010000000
VERSION     DAORKW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210_length_8719_cov_6.296745"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLB46_05235"
     CDS             <1..719
                     /locus_tag="PLB46_05235"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HRF75950.1"
                     /translation="PHNLFLTLKQINELGGVVKRGEHSCPVVFWSQKEVAKEGTDEKE
                     TKRILRYYSVFNIDQCLHIPEEKIPELHLNDNVLLSTCEAIVENMPQKPKIQHKGQEA
                     YYSPPKDVINMPKLQTFVDSEAYYTTLYHELVHATGHASRLNRDTLTKPSKPGEEAYS
                     EEELIAQVGACFLAAQSGCSLKHFPNDVAYIQGWLSRLKGDKRLLLFASSKAQRAVDF
                     ILNNQLHEMREDIGNEETAD"
     gene            1333..5202
                     /locus_tag="PLB46_05240"
     CDS             1333..5202
                     /locus_tag="PLB46_05240"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRF75951.1"
                     /translation="MKTQIQFYKSLLILIALTCNLNANGQLYLIEDWVQSTGIPDTID
                     YSASKVDGSGNIYVTTNTISVTEKANILTTKYNSSGVVQWEVENDNADENDYGTAIEV
                     DGSGNVYVGAATWVDATNKYDYIVIKYNSSGTQQWTATYNGPGNFYDIPTDIYVDGSG
                     NVYVTGYSYGSTTLGDYCTIKYNSSGTAQWTSRYDYASDQDAAAIVRSGPGSTVVVIG
                     ASENIPGDWDFAAVQYNTSTGAQVNTNRNSASGSGFDQVYGADVDASGNIYITGRASV
                     VDEGFNMRTVKLEPDLDLVWAKNHDHAELDDEAHGVIVDLDDNVYVSGWVTKEDQTRS
                     MLLVKYNSAGTLQWYKQIDAPNSGLDAVGLKISSVTGGNVVIAGNVDNGQSLDFLTAI
                     YNSDGDLLWLEQYDSPDKYDDKVNFVKADVDGVFYVGGKSYGISTATNRLIKYNSVSY
                     IIPPDDEMEHTSSFTFFENKGQIKDTEDEIRNDIKYYTHRHNPSLYFSDDVLSYVWSR
                     IDTNTTINDTLARVDLTFTGSNTSKSIHRAVSQGGEYLNYYLPHCPDGVTNARSSDRL
                     IISELYDYVDLEYYFDNAGLKYYLIIKPGWSEQADPISLLYDGATDVNILGGGELEVI
                     TSLGKLTHSIADAYQIDASGEFVALGWDAEFLSIDDFEIGFDIDVYDDELPLIIEMKL
                     EGALGGADCEDNVIWGTWNGGTGDEYMMDITTGYVHPAYTKLFTTGNTRSNDFPAELL
                     FIGELSPFRDIFIEKFNLDNAGGMDKYEPNWSTYVGGSGNDGNTSVVLTDLSSDIVFA
                     GQTLSDDLPVVEFADAYFDNDQNTFTDGVLGKLDFGGNLNWLTYFGGDEAINTIYGIE
                     TTVENGLIIVGRSHGGAEFPFVNPGGGSPYYTSGKSGFIVELDETLQPVWGTLFGGSD
                     VYYDWLTDVAIDQNRDYIFCGQVSSSGLPTTAGAFQETYSGNQDAYILKIHPDGAYRT
                     KVWCTYYGGYGSEDPTALIADNDNNIFIVGSVNDHDVDDIDAFPRYFAGGCPECFYDD
                     VYSPSGEFESEGFLIKLNDDGNRLWATYFGGTTGNVIRDIAKMGDYIMVTGNLNLDID
                     EFPLVEVDGVYFDDVNENATSYISMFNKSTELVWSTFLGDNATNGGIALTTDNTHPEV
                     YMVGNISFGTELNDFKPLCDPGGDAFYQDTPLGIATGTDVYKNYDGYILGFDISMFES
                     YISLQNNEDISGLELFPNPTTDIINIITGFEITEVNVFDILGRNIISRQIGSKFCQVD
                     ISQFNAGTYLVQVVTRIGTLNKLIVLE"
     gene            5208..6476
                     /locus_tag="PLB46_05245"
     CDS             5208..6476
                     /locus_tag="PLB46_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75952.1"
                     /translation="MQKYFYLIIFMVLNLSCFSQWELIYENDDVGCLSDMIFTTPING
                     YIVGGQKHILTTINAGATWIVDSSYDGSDFRIIDFINQDTGLICCNPYSGDGNVLITN
                     DGGATWNVPILDNSVNFADMELVSGGNVIYAGVTGSFGYSFISENYYSNNIWSTIVDP
                     ATNIWTITFLNENIGYLTGEFSSDIAYATIYKTVDGGYTWYINENMYGPLYSVSFPSD
                     SIGYGLGYESRVWKTIDFGESWTMLPYDFGGYDYNDIDLWISGIYFYNDTIGYLKATY
                     NDLDKVEILRTFDGGISWNPTVIDDPEFNDMGHFFCTGLDTCYAISCYKIYKTTDGGG
                     KDTTTSINTTPNYDVCYIYPTLSSSLITLKNPQNIELLEITTRSIWGQEIDLFFSENK
                     TADISKLMNGIYITDIYTVSGRQVIKWVKY"
     gene            6690..7334
                     /locus_tag="PLB46_05250"
     CDS             6690..7334
                     /locus_tag="PLB46_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75953.1"
                     /translation="MNAFEIVFEKAYEDGFILYTNEAQKSAEESLHSFLFNQYLQETF
                     NISITEDNYLRYCTYENFYNYLSNYYQLPRFNLSLTAIEKIDTVLKFFISDKNLRPRI
                     LGHVIASRIVTKESLMSYDDLQRCLNQLVKDGFITVEIDVNKKAEYLITWDGEYFCEI
                     GGYQNDVKLDLDAKEAPIKTAKMQHQLNRLTWILAIAAGISAIYYLIQIVEIFN"
     gene            complement(7415..8356)
                     /locus_tag="PLB46_05255"
     CDS             complement(7415..8356)
                     /locus_tag="PLB46_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRF75954.1"
                     /translation="MNRKKPSQKIEADVMFKSNRECCICKKPKRGDHIHHIDGNSSNN
                     KLENLALLCFKHHNEASISGNISKKLSPQTIIRYREYKYLVVNTKRQNALKVFNSSLG
                     DLSTEDLLIASKNAIIILKLEKIKEEYFRSNWKQRDKVLNKLTKYSNHSNYRVALDVY
                     KFLNLVADGTRSGMTSGISFSILFLVMNYFPDTENIDDNQKIIELAEICIHISFSLVY
                     DMFIYLKDYDIAQYGLLILKFINQVGKRYKLPVITEKVSNIYIELQDAIKRPDRPDLI
                     NAQKIITLFYDDVNEGTITLPELPSDIMEVIQLNRPT"
BASE COUNT         2887 a         1279 c         1693 g         2860 t
ORIGIN      
        1 acccccacaa tctgttcctc acccttaaac aaattaacga actaggtggt gtggtgaaaa
       61 gaggagaaca ttcctgtccc gtggtatttt ggtcacaaaa ggaagtcgct aaagaaggga
      121 ctgatgagaa agaaacaaaa cgaatactcc gatattattc tgtatttaac attgatcagt
      181 gtttgcatat tccggaggag aaaataccgg aactgcactt gaacgacaat gtattgcttt
      241 ctacctgtga ggcaattgta gaaaacatgc ctcaaaagcc caagattcaa cataaagggc
      301 aggaagcgta ttacagccct ccaaaggatg ttattaatat gcctaagcta cagacctttg
      361 ttgatagcga agcatactac acgaccttat atcacgaatt agtgcatgct acaggccacg
      421 caagccgttt aaacagagat acgctcacca aaccatcaaa gccaggtgaa gaagcatatt
      481 ctgaagaaga actcattgct caagttggtg cctgtttctt agcagcacaa tcaggttgtt
      541 cccttaaaca ctttcctaac gatgttgcct acatccaagg atggctcagt cgcctaaagg
      601 gtgacaaacg cctcctcttg tttgctagtt caaaagcaca acgggctgtg gattttatcc
      661 ttaataacca actgcatgaa atgcgggaag atattgggaa tgaagaaact gcagattgat
      721 tgcagctttg ttgtgttttg cttgcaatgc ttgtttaacc cctaattggg atgtatggtg
      781 tgaacccaca gccgaaatgg tctgtgggtt ttttatattt tttcaatcaa atgttgtttg
      841 ggaatatata aaaatctatt tttatattgt gttttcatgg ggatagcgag ggttatcgga
      901 gtccttccga gcccttgctt tttttgtttt cgattttcta catagataca tctcaaagaa
      961 aaataaaaaa gagaaaaaaa taaaaagaaa ggaacttact cattatgacc ggaacatatt
     1021 tttcaggagg aacggaataa agcgctggcc tttttgtgcg ctggcttttg cgtttatgcc
     1081 ggaattcctg aaaaatatgt gaagggcata atacctttgt ggagagattt tatttcttag
     1141 gcagaatcgg acaacctttg cgaagattga ttatattaaa tttaatatta ttagtgtcta
     1201 ttatttagga ttcacggaaa accgtgaatt tgactataaa tgacaataat ttaactttta
     1261 atttttgaca atctattttt ttataactaa ttttcataat tatttttaaa aaacccttta
     1321 aattcttttg atatgaaaac tcaaatacaa ttctacaaga gcttattaat tctgatagca
     1381 cttacatgta atttaaacgc aaatggacag ctttatttaa tagaggactg ggttcaatct
     1441 actgggatcc ctgacactat agattacagc gcgtcgaaag ttgacggctc tggtaatatt
     1501 tatgtaacga caaatactat ttctgtaacc gaaaaagcaa atattttaac aacaaaatac
     1561 aattcatccg gtgtagttca gtgggaggta gaaaatgata atgctgacga aaatgattac
     1621 ggaactgcaa tagaggttga tggttcagga aatgtatatg ttggtgcagc tacttgggta
     1681 gatgcaacaa ataaatatga ttatatcgta ataaaatata attcgtctgg aacacaacaa
     1741 tggactgcaa cttataatgg cccaggtaat ttttatgata tccctacaga tatttatgta
     1801 gatggttcag gtaatgttta tgtgacaggt tatagttatg gtagcacaac acttggtgat
     1861 tattgcacaa taaaatacaa ctcatcaggc accgcacaat ggacttcaag atacgactat
     1921 gcatcggatc aagatgcagc agcgattgta agaagcggac ctggttcaac tgttgttgta
     1981 attggcgcaa gtgaaaatat tcccggagat tgggacttcg ccgctgtgca atataacact
     2041 agtactggcg cgcaagtaaa tacaaatcgc aactcagcct ctggtagcgg ctttgaccaa
     2101 gtatatggtg cggatgtgga tgcttcagga aatatctata taacgggtag agcttcagtt
     2161 gtagatgaag gatttaatat gcgtactgtt aaattagaac ctgaccttga tttagtttgg
     2221 gctaaaaatc atgaccacgc agaattagat gacgaggcac atggagttat tgtggattta
     2281 gatgacaatg tttatgtgtc cggttgggtt acaaaggaag atcaaactcg ttcaatgcta
     2341 ttagtgaaat ataatagtgc tggcacactt caatggtata agcaaattga tgcaccaaat
     2401 tcaggtctgg atgccgtagg actaaaaatt agtagtgtta caggtggaaa tgttgttatt
     2461 gctggtaatg tagataatgg tcaaagttta gactttttaa cagcgattta taattctgat
     2521 ggagatttgc tttggttgga gcaatatgac agccctgata aatatgatga taaggtaaat
     2581 tttgttaagg cagatgttga cggagtgttt tatgttggag gtaaatctta tggaatttct
     2641 acagccacaa atcgactaat taaatataat agcgtgagtt atataattcc acccgatgac
     2701 gaaatggaac atacttcatc attcaccttt tttgaaaata aaggacaaat aaaagatact
     2761 gaggatgaga ttagaaatga tataaaatat tatactcatc gtcataatcc ttcattatat
     2821 ttttcagatg atgttttaag ttatgtttgg tcgcgaatag acacaaacac aacaataaac
     2881 gacactttag ccagagtaga ccttactttc accggaagca atacgagcaa atctatacat
     2941 agggctgtga gtcaaggagg tgaatattta aattactatt tgcctcattg tcccgatggt
     3001 gtaacaaatg cacgtagtag tgatagatta attatttctg aattatacga ttatgtagat
     3061 ttagaatatt atttcgataa cgccggttta aaatattatt taatcattaa accaggatgg
     3121 agcgagcagg cagaccctat atcgctttta tatgatggcg caacagatgt taacatttta
     3181 ggtggaggtg aacttgaagt aattacttca ttaggtaaat taacacattc aattgcggat
     3241 gcatatcaaa ttgatgcaag cggagagttt gtagcattag gttgggatgc tgaatttttg
     3301 tctattgatg attttgaaat aggctttgat attgatgtat atgatgatga attacctttg
     3361 attatcgaaa tgaagttaga gggggcatta ggtggtgctg attgcgaaga taatgtcatt
     3421 tggggcactt ggaatggtgg tacgggtgat gagtatatga tggacataac aacaggatat
     3481 gttcatccag cttataccaa attatttaca acaggtaata cacgatccaa tgacttccca
     3541 gcagagttat tatttattgg tgaattaagt ccgtttcgag acatatttat agaaaaattt
     3601 aatcttgata acgcaggtgg aatggataaa tacgagccaa attggagtac ctacgtagga
     3661 gggtcaggta atgatggaaa tacttcagtc gtgttaactg atttaagttc ggacattgta
     3721 tttgctggac aaacacttag cgacgattta ccagttgtgg aatttgcgga tgcttatttt
     3781 gataatgacc aaaatacttt tactgatggt gttcttggaa aattagattt tgggggcaat
     3841 ttaaattggc ttacttattt tggtggagat gaggcaatta atacaattta tgggattgaa
     3901 acaacagtag aaaatggact aattattgtt ggtaggtctc atggaggagc tgagtttcca
     3961 ttcgtaaatc caggtggggg cagtccttat tatacttcgg gcaagtccgg ttttattgta
     4021 gaattagatg aaacactcca acctgtttgg ggtactttat ttggcggttc agacgtttac
     4081 tatgactggc ttacagatgt agcaatcgat caaaatagag actatatttt ttgtggacaa
     4141 gtaagttcga gcggtttgcc gactactgct ggagcatttc aagaaactta ttcaggaaac
     4201 caagatgctt acattttgaa aatccatcca gatggtgcct atagaacaaa agtttggtgc
     4261 acctattacg gtggatatgg ttctgaagat cctacagcac tcatagcaga taatgataat
     4321 aatattttta tagtcggttc cgtaaatgac cacgatgtgg atgatataga tgcattccct
     4381 cggtattttg cgggaggatg cccagagtgc ttctacgatg atgtttattc accatctggt
     4441 gaatttgagt ccgaaggttt tttaataaaa ttaaatgatg atgggaatcg tttatgggca
     4501 acttattttg gtggaaccac aggaaatgtg attcgcgata ttgctaaaat gggcgattat
     4561 attatggtaa ctggcaactt aaatcttgat atagatgaat ttccattagt tgaagtagat
     4621 ggagtatatt tcgatgacgt aaacgaaaat gcaacttctt atatttcaat gtttaataaa
     4681 tcaacggaat tggtttggtc tactttttta ggagataatg ccactaatgg tggtattgct
     4741 ttaactactg acaatacaca tcctgaagtt tacatggttg gaaatataag ttttggtacc
     4801 gaattgaatg attttaaacc actttgcgat cctggaggag atgcattcta tcaggataca
     4861 cctttgggta tagcaacagg aaccgatgtg tataaaaatt atgatggcta tattttaggt
     4921 tttgatattt cgatgtttga atcttatatt tcattacaaa ataatgaaga tatatctggc
     4981 ttagaattat ttccgaatcc aaccacagac ataattaaca ttattacagg atttgaaata
     5041 actgaagtaa atgtattcga tattttagga agaaacataa tttcaagaca aattggatca
     5101 aaattttgcc aagttgatat aagccaattt aatgctggaa cttatcttgt acaagtggtg
     5161 acccgaattg gaactttgaa taaattaatt gtattagaat aaatgtaatg caaaaatatt
     5221 tttatttaat aattttcatg gtattgaatc tatcatgttt ttctcagtgg gagttgatct
     5281 atgaaaatga tgatgtagga tgtttaagtg atatgatatt tacaactccg ataaatggtt
     5341 atatagttgg gggccaaaaa catatcttaa caacaataaa cgcaggagca acttggatag
     5401 ttgactcatc atacgatgga agtgacttta gaataataga ctttattaat caagataccg
     5461 gtttgatttg ttgtaatcca tattctggcg atggaaatgt attaattaca aatgatggtg
     5521 gagcaacttg gaatgttcct attttagata attcagtgaa ttttgccgat atggaattag
     5581 tctctggagg taatgtaatt tacgcagggg ttactggttc ttttggctat agttttatct
     5641 cagagaacta ttattcaaat aatatttggt caacaattgt agacccagct actaatattt
     5701 ggacaattac tttcttgaat gaaaacattg gctatttaac tggagagttc agttcagaca
     5761 tagcttatgc gactatttat aaaaccgtgg acggtggtta cacatggtat attaacgaaa
     5821 acatgtatgg cccactttat agtgtttcct tcccatctga ttctataggt tatggattag
     5881 gttacgaaag ccgagtttgg aagacaattg attttggtga aagctggacg atgttaccat
     5941 acgattttgg tggatatgac tataatgata tagacttatg gatttcaggg atctactttt
     6001 ataatgatac gattggatat ctcaaagcta cttacaatga tctagataaa gtagaaatat
     6061 tgcgcacatt tgatggcggg attagttgga acccaacagt aatagacgac ccagaattta
     6121 atgatatggg gcatttcttt tgcacaggat tagacacttg ttatgcaatc agttgttaca
     6181 aaatctataa aacaactgat ggtggtggga aggacactac aacatcaata aataccactc
     6241 caaattatga tgtatgttac atttatccca ctttatcaag ctctttaata actttgaaaa
     6301 atccacaaaa cattgaatta ttagaaatta caacaaggtc aatttggggt caagagattg
     6361 atttattttt tagtgaaaat aaaactgcag acataagtaa attaatgaat ggtatttata
     6421 taaccgatat atatacagtt tctgggagac aagtaataaa gtgggtaaaa tattgatact
     6481 taaaatatac catgcataaa gctaaataga tttccgtata atcagatacg tatatcgatt
     6541 atggtgtatc ccgaaaatat tatatagcag tgtgaatgat atataactat tcttaaaaga
     6601 gcgtggttta acgccaaaca aagaaataaa tatatttaac ctaagaatta ctttttgaat
     6661 aattagaaaa agaaataata gttttgaata tgaatgcatt tgagatagtt tttgaaaagg
     6721 cctatgaaga tggttttatc ctctatacta atgaagcgca gaaatcagca gaagaatcac
     6781 ttcatagttt tttatttaat caatatttgc aagaaacctt caacatttca ataacggaag
     6841 acaactatct tcggtattgc acttacgaaa acttttataa ttacctatca aattactatc
     6901 aactgccacg gtttaatctg agtcttacag ctattgaaaa gattgatacc gtgctaaagt
     6961 ttttcatttc tgataaaaat ttaagacccc gaattttggg acatgtaata gcaagtcgta
     7021 ttgttacaaa agaaagtttg atgagctacg atgatttaca aaggtgtcta aatcagttag
     7081 taaaagatgg ttttattaca gttgaaattg acgttaataa gaaagctgaa tacttaatta
     7141 cttgggatgg tgagtatttc tgcgaaatag gtggttacca aaatgatgtt aagctcgatc
     7201 ttgatgcaaa agaagctcca atcaaaacag ccaaaatgca acatcaatta aatagattaa
     7261 catggatact tgcaattgcg gcggggatta gtgctattta ttatttaatt caaattgttg
     7321 aaatctttaa ctaagaacaa ggttgtataa attaagattt agactctcaa atttctcagt
     7381 aataccttca ttatttaatt aggatgtgaa aatattaagt aggtctattt aactgaatta
     7441 cctccattat atctgaaggt aattctggta atgtaatggt accttcattt acatcgtcat
     7501 aaaacaatgt aatgattttt tgggcgttaa ttaaatccgg tcgatcaggt cttttaattg
     7561 catcctgtag ttctatgtaa atattcgaaa ccttttctgt aataacgggc agtttatagc
     7621 gtttacctac ttgattgata aacttcaaaa tcaataatcc atattgagca atatcgtaat
     7681 ctttcaagta tataaacatg tcataaacca agctaaaaga gatgtgtatg cagatttctg
     7741 ctaattcaat aattttttgg ttgtcatcta tattttcagt gtctggaaag tagttcataa
     7801 caagaaaaag tattgagaag gaaatacctg aagtcatacc acttcttgtt ccatcagcaa
     7861 ctaaattgag aaatttgtaa acatctagtg caactctata atttgaatga tttgagtatt
     7921 ttgtaagctt attaagaact ttatctcttt gtttccaatt cgacctgaaa tattcttcct
     7981 taattttctc gagcttaagt ataataattg catttttaga agctattaat aaatcctctg
     8041 ttgataaatc ccccaaggag gaattaaaaa ccttcaatgc attctgtctc ttagtgttta
     8101 ccactagata tttatactct cgatatctaa taatggtttg cggagaaagt ttttttgata
     8161 tatttccgga aattgaagcc tcattatggt gtttaaaaca aagtaaagcc aaattttcta
     8221 atttgttatt agatgagtta ccatcgatat gatgaatgtg gtcgccacgt ttcggttttt
     8281 tacaaataca acactctctg ttggatttaa acattacatc tgcttctatt ttttgtgaag
     8341 gtttttttcg gttcactaat tttaatttat tatttaaatt attagtgcta ttgaataaaa
     8401 gataaatttt gttgtcttct gcctgaatac ttaatcaagc cctcctgaga aagttcttga
     8461 agaatacttc taatttcttc aattgaggca ttaggaagaa gatgctcaat tttgtgttca
     8521 ttactatcag attcatgttg tttgaggcac atgagtaaat tcagttaact gctcattttg
     8581 atatttaatt ataaaattaa taaaaattca aattattaaa ctgattaatc aacaattaca
     8641 attattgact aaaaaactgc tcgccgttaa agcgaaatga atgaagtata acgtaatgaa
     8701 tggagctgcg agcggagat
//
LOCUS       DAORKW010000088         8698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_212_length_8698_cov_6.092213, whole genome shotgun sequence.
ACCESSION   DAORKW010000088 DAORKW010000000
VERSION     DAORKW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212_length_8698_cov_6.092213"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /gene="radA"
                     /locus_tag="PLB46_05260"
     CDS             complement(<1..1289)
                     /gene="radA"
                     /locus_tag="PLB46_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRF75955.1"
                     /translation="MAAKAKTAFFCRNCGFESPKWQGKCPSCAEWNTFTEEVIERPDD
                     KEVWKDEKPDKKGNFPLALDEITAGDTVRIDTGDLELNRTLGGGIVPGSLVLVGGEPG
                     IGKSTLMLQLALTLKKLKVLYVSGEESEQQIKMRADRLKGKNANLFISTEVAINKLFK
                     QIKQLGPELVIIDSIQTLVSDFVDSTAGSIAQIKECAGQLMRFSKESNVPVFIIGHIN
                     KEGYIAGPKILEHMVDSVLQFEGDRNYTHRILRTIKNRFGSTSEIGIYKMEGDGLQQV
                     NNPSELLITEKEEPLSGVAIAATMEGMRPMLIEVQALVSPAVYGNPQRTATGFDLRRM
                     NMLLAVLEKRGGFAFGNKDVFLNIAGGLRVEDPAIDLSIACALLSSISDIALPLNICF
                     AAEVGLSGELRGVSRIEQRITEAEKLGFDTIYISKHN"
     gene            1347..2891
                     /locus_tag="PLB46_05265"
     CDS             1347..2891
                     /locus_tag="PLB46_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRF75956.1"
                     /translation="MDRSEQFKQTITEGYNFQGKSIILGTAILDHQPLEQTHVKVPLR
                     MMNRHGLVAGATGTGKTKTLQGIVEQLSDNGVGVLIMDIKGDVSGIAMSGTPNPKIDE
                     RMQKIGKTWQARNYPVELMSISQEKGVKLRATVSEFGPVLFSKILELNDIQEGVVSLV
                     FKYCDDNGLPLLDLKDFRKTLNFLTNEGKAEIESNYGKISTSSAGTILRKIIEIEEQG
                     ADVFFGEKSFDVEDLCRINDKGQAYINILRLTDIQSKPKLFSTFMLCLLAEVYQTFPE
                     IGDKEDPKLVIIIDEAHLIFNEASKSLLEQIQTIVKLIRSKGVGIFFCTQLPTDIPPE
                     VLSQLGLKIQHALRAFTANDRKAIKLTSENYPLTDYYKTDELLTNLGIGEAIVSALNE
                     KGAPTPLAHTLLVAPQSRMDILSDDEMNTILQNSALVKNYNESIDRESAYEMLNKKLE
                     ASVKAAEKEKELVEKEKQIKQENREKKIKQHEDKPLIDNQTKKRLVNSAITLVQRGLL
                     GLLKRR"
     gene            2931..3929
                     /locus_tag="PLB46_05270"
     CDS             2931..3929
                     /locus_tag="PLB46_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF75957.1"
                     /translation="MKQLTFAGMVIMLLFTACVPQRQFQDVVAARDKCLQDTAVMASQ
                     LRARNTEVAQLEEQIKQMQSSMSLMRKDSVDIHSRYDQMVKANEQLRQIEDMLNNRIN
                     QLLALSSTENQQLREDLQRKSDELLLKEKELNNKAQILTDQQKSIETLNKDLEARSKR
                     VQELEDILNKQEQTVIDLKNTIDKALSGFSSSDLTVERKNGKVYVSLSEKLLFASGST
                     VVDPKGKDALKKVADVLKTNGDITVEIEGHTDNVPLKSANFPKDNWDLSVLRATSIVK
                     ILTVDNGVDAKRVTAAGRGEYFPVADNGTTDGKSKNRRTEIILSPNLDALYDLLNK"
     gene            3957..4778
                     /gene="tatC"
                     /locus_tag="PLB46_05275"
     CDS             3957..4778
                     /gene="tatC"
                     /locus_tag="PLB46_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRF75958.1"
                     /translation="MAAIKKNKDEMSFMDHLEDLRWHLVRIVAVVILFSIVFLIYPDF
                     IFDKIILAPKSPDFVTYKIFCDIGERFNIGGLCYTQLDYELFYLNLSSGFLFYIKIAF
                     MAGFIFAFPYILWEIWRFVKPALHDNEKSNLGGVIIISTLLFYVGVFFGYFLMAPFSI
                     NFLATFSLSDQIENQFTFDSYLGILTGMVLWTGVIFEIPMVAYFLAKLGLLGREFLAN
                     NRKYAFIVSLVLAAIITPSGDAFSLFLVAMPLWILYEVSIVVVMVVEKRKLKQVD"
     gene            5332..5772
                     /gene="rpiB"
                     /locus_tag="PLB46_05280"
     CDS             5332..5772
                     /gene="rpiB"
                     /locus_tag="PLB46_05280"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRF75959.1"
                     /translation="MQNKTIAIGCDHAGFPYKEGIINYLTSNGYTVKDCGTSSLDSVD
                     YPDYVHPLAQSIESGESAMGVLICGSGNGVCITANKHQQIRAAMVWREDISALSRQHN
                     NANVICIPARFITLEEAVNFVHVFLHTEFEGGRHATRVNKITQF"
     gene            5786..7357
                     /locus_tag="PLB46_05285"
     CDS             5786..7357
                     /locus_tag="PLB46_05285"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRF75960.1"
                     /translation="MKYSALFFALLIATNCVFAQTEDLGIKYANTITASELENHLTIF
                     ASDYYEGRETGTRGLSRAADYLSKQFTLSGVPPLQANGGYFRDYPLIQYQWTNSTISS
                     YKNLFTIMTDFYGYAGANNSFAYTANDIVFLGYGIDDSMYSDYKNVDVKGKIVLIAAG
                     EPMVNGKSVITGSDSLSAWSKDWRKKAAAATSNGVMCLLTIDPKLAEVLSNPQWKNFL
                     EGSILKLQSEYKQPEYTNNLFISQNMADKLLGKKKKFIQKTIDKISTTGKPANFLVKN
                     TIVFNVVKTENTIYADNVLAFVEGTDLKEEVIVVSAHYDHLGKKDTVVYNGADDDGSG
                     TIALLEISEALAKAKSNGEGPRRSVLFIAFSGEEKGLLGSKAYTDNPAIPFENTVANL
                     NIDMIGRVDEKHTATPNYVYIIGSNFLSTALHSINEAAAKNYTNIELDYKYNSTTDPN
                     RYYYRSDHYNFAKNNIPVIFYFNGTHEDYHQPTDDVEKINFPVYEARCRLVFHTLWIL
                     ANQDARIKVDVAQED"
     gene            7390..>8698
                     /locus_tag="PLB46_05290"
     CDS             7390..>8698
                     /locus_tag="PLB46_05290"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRF75961.1"
                     /translation="MTKLIALFTFLFISIAAFGQTVYTVETIPDPKQTGGGYVSNPDG
                     VIDASTVQNINAVLAALEDSTTIQVAVVVVNSIGDEVPGDFRTKLFRHWGIGQADNNN
                     GLLILLVMDQRRMEFEVGYGLEGILTDIMCVRIQQTYMVPLAKEGRMSEAVLAGVMQV
                     NQILTDPQYRDEVYADSLNSSSNNAWWKDPVSGMGLGVIGGIYALIAYAIFANRKSSL
                     KKAPSYVKNNYSDAYTKTKFGLLNIGAPAGLLAWQEIEGSLRIFEFAAILYGIFLIIL
                     LEKRFRLNKYILKEGNTLEPQETYNLFSKSHSNGWLAATIFFPLPFVLYNLFNKGRMN
                     KLRNTPPIADSGIAMVKMDEKEDDGFLKAFQIKEEELRSVDYDVWIENATHQVKIYRF
                     ENYNSRYKPCPNCQSITFLMTKNETLVSPSYTSSGTGQKTYTCK"
BASE COUNT         2791 a         1510 c         1708 g         2689 t
ORIGIN      
        1 atattatgct tagaaatata aatcgtatca aaccctaatt tttctgcttc agtaattcgt
       61 tgctcaatac gacttacacc gcgcagctca ccactcaatc cgacctcagc agcaaaacaa
      121 atatttaaag gcaatgcaat atcactaata gatgataaca atgcacaagc aatacttaaa
      181 tcaatagcag gatcttccac ccgcaaacca cctgcaatat ttaaaaacac atctttattg
      241 ccaaatgcaa acccgcctcg tttttctaat actgcaagta acatattcat tcttcgcaaa
      301 tcgaaaccgg ttgcggtgcg ctgcggattg ccatatactg ccggacttac caatgcctgc
      361 acttcaatca acatcggtcg cataccttcc atagttgcgg caattgcaac accactcagt
      421 ggttcttctt tttcggtgat taataattcg ctcggattat ttacctgttg caaaccatca
      481 ccttccattt tataaatacc aatttcgcta gtagaaccaa atcgattttt gatagtgcgc
      541 aaaatacgat gcgtataatt tctgtcgcct tcaaattgta acacactatc taccatatgt
      601 tccaaaattt ttggtccggc gatataacct tctttattga tatgcccaat aataaatacc
      661 ggcacattac tctctttcga aaatcgcatt aattgtccgg cacattcttt tatttgcgca
      721 atacttcctg cagtggaatc aacaaaatca gaaactaaag tttgtatcga atcaataatc
      781 accaactcgg gaccaagttg ttttatttgt ttaaataatt tattgattgc tacttctgtt
      841 gaaataaata aattggcatt tttacctttc agtctgtcgg cgcgcatttt aatttgctgc
      901 tcactttctt caccacttac atataatact ttgagttttt ttaaggtaag tgccagttgc
      961 agcatgaggg tcgatttgcc gattccgggc tcaccaccaa ccaaaaccag gctacccggc
     1021 acaattcccc caccgagggt tcggtttaac tctaggtctc cggtatcaat tctaacggta
     1081 tcgcctgctg taatttcatc cagcgcaaga ggaaaattgc cctttttatc gggtttttca
     1141 tctttccata cctctttatc gtcagggcgc tcaattactt cttccgtaaa agtgttccat
     1201 tccgcacaac tcgggcattt gccctgccat ttcggcgatt cgaatccgca attcctacaa
     1261 aagaatgctg tttttgcttt tgctgccatg tatttaccac accaaatttt aaggcgtatc
     1321 tttgccgcaa attaaaacat ttttttgtgg atcgttcaga acagttcaaa caaacgataa
     1381 ccgagggcta taatttccag gggaagtcca tcattttggg gacagccata cttgaccatc
     1441 agcctttaga acaaacacat gtgaaggtgc ctttgcgcat gatgaaccgt catggattgg
     1501 tggctggagc tacaggaaca ggaaaaacta aaacccttca gggaatagtt gagcagttga
     1561 gcgataatgg cgtcggcgtt ttaattatgg atattaaggg tgatgtgagt ggtattgcca
     1621 tgtcaggaac acctaatcca aaaattgatg agcgcatgca aaaaatcgga aaaacctggc
     1681 aagccagaaa ttatccggtg gaattaatga gcatcagtca ggaaaaaggt gtaaaattac
     1741 gcgctaccgt ttcggagttc ggaccggttt tattttccaa aattttagaa ttaaatgata
     1801 ttcaggaagg tgttgtttcg ctggtattta aatattgcga cgacaacgga ctgcctttgc
     1861 tcgatttaaa agactttaga aaaacgctca attttttaac caacgagggc aaagctgaaa
     1921 ttgaatcgaa ttacggaaaa ataagtacat cgagtgcagg aactattttg cgtaaaataa
     1981 tcgaaataga agagcagggt gcagatgtgt tttttggaga aaaaagtttt gatgtggaag
     2041 acctttgtcg tatcaatgat aaaggacagg catatattaa tattttgcgg ttaacggaca
     2101 tacaaagcaa accgaaattg ttttcaactt ttatgttgtg tttgctggca gaagtatatc
     2161 agacttttcc ggaaattggt gacaaggaag accctaagct ggtaattatt attgatgaag
     2221 cacatttaat ttttaatgaa gcgagtaaaa gtttattgga gcaaattcaa acgattgtaa
     2281 aattaattcg ctcgaaagga gtgggtattt ttttctgcac gcaactgcca acagatattc
     2341 cacctgaagt tttatcgcag ctggggttaa aaattcagca tgccttacgt gcatttactg
     2401 ctaacgaccg caaggccatt aaattaacgt cagaaaatta tcctttaacg gattattata
     2461 aaacagatga gctgttaacc aatttaggta ttggagaggc gattgtaagc gcattaaatg
     2521 aaaaaggcgc acctacaccg cttgcgcaca ctttattagt tgcaccgcag tcgagaatgg
     2581 atattttaag tgatgatgaa atgaatacca ttttacaaaa ctccgcgttg gtaaaaaatt
     2641 ataatgaaag tatcgacaga gagagtgcgt atgaaatgct caataaaaaa ttggaagcaa
     2701 gtgtaaaagc tgcggaaaaa gaaaaagaac ttgttgaaaa agaaaaacaa ataaagcagg
     2761 aaaacagaga gaagaaaatt aaacaacacg aagacaaacc attaatagat aatcaaacaa
     2821 aaaaacgttt agtaaattcg gcaatcacac ttgtgcaaag aggattgtta ggattattaa
     2881 aacgtcgtta atttaaccga taaaatatta taaataaaat gaacaaccgt atgaaacaat
     2941 taacattcgc aggcatggta ataatgttat tatttactgc ttgcgttccg cagcgtcaat
     3001 ttcaggatgt tgttgctgcc agagataaat gtttgcaaga cactgctgtt atggcaagtc
     3061 agttgcgcgc aagaaatacc gaagttgccc aactggaaga acaaattaaa caaatgcaat
     3121 ccagcatgtc gctgatgcgc aaagactctg tggacatcca tagtcgttac gaccaaatgg
     3181 taaaagcgaa tgagcaattg cgtcaaattg aggatatgct aaataacaga ataaatcagt
     3241 tattggcatt atcatccact gaaaatcaac aattacgtga agatttacaa cgcaagtcgg
     3301 atgagttatt attaaaagaa aaagaactta ataataaagc acaaatatta accgaccagc
     3361 aaaaaagtat tgaaacatta aacaaagatt tagaagcgag gtctaaacgc gtgcaggaat
     3421 tggaagatat tttaaacaaa caggaacaaa cggtaattga tttaaaaaac actatcgata
     3481 aagcattgtc cggctttagt tccagcgact taactgttga gcgtaaaaat ggcaaagtat
     3541 atgtttcatt aagcgaaaaa ttgttgtttg cttcaggcag tacagttgtt gacccaaaag
     3601 gaaaagatgc tttgaaaaaa gtagctgatg tattaaaaac aaatggcgat atcactgttg
     3661 aaattgaagg acataccgat aacgtgcctt taaaaagtgc taattttcct aaagacaact
     3721 gggacttgag tgttttacgt gcaactagta tcgtgaaaat tttgactgta gataatggcg
     3781 tagatgcaaa aagagttact gctgccggtc gcggtgaata tttccctgtt gccgacaatg
     3841 gaacaacaga tggcaaatct aaaaacagaa gaacggaaat tattttaagt ccgaacctcg
     3901 atgccttata cgatttgtta aataaataat tagaaaagat aatttaggat aagttaatgg
     3961 ctgctattaa gaagaataag gatgagatgt cgtttatgga ccatctcgag gatttgcgtt
     4021 ggcatctggt gaggattgtt gcggtggtaa ttctgtttag tattgtattt ttaatttatc
     4081 cggattttat tttcgacaaa ataattctcg caccaaaaag tcctgatttt gtcacctaca
     4141 aaatcttttg tgacattggt gaacgattta atatcggcgg gttatgttat acgcagctcg
     4201 attatgagtt gttttatttg aatttatcat caggattttt attctatata aaaatcgcat
     4261 ttatggcggg ttttattttc gctttccctt atatattgtg ggaaatatgg cgatttgtaa
     4321 agcctgcttt gcacgacaat gaaaaaagta atttgggagg ggtaattatc atttccacat
     4381 tactttttta tgtcggcgtg ttttttgggt atttcctcat ggctcctttt tctatcaact
     4441 ttttggcaac atttagttta agcgaccaaa ttgaaaatca atttacattt gattcttacc
     4501 tcggtatttt aaccggcatg gtattgtgga cgggtgtaat ttttgaaatt ccgatggtgg
     4561 cttatttcct tgcgaaattg ggattgttag gaagagaatt tttagccaat aatcgcaaat
     4621 atgcatttat agtttctctc gttttggctg cgattataac accttccggt gatgcattta
     4681 gtttgttttt agtagccatg cctttgtgga ttttgtatga agtgagtatt gtggtggtaa
     4741 tggtggttga aaaacgtaaa ctcaaacagg tagattaaag tgtttgtttc acgcgaaggc
     4801 gcggagggga ttgatgaatt gatgtgtgat atggtttgtt tttgagttcg cggagctgat
     4861 aaaccagttt agtggttgtt ttttttaatt tccttaccat ctccgctttc tcccttctcc
     4921 gcgtgaaata ttcacacata taaaaattat ttgtttctcg caaagtcgct aagttttaaa
     4981 aaaaagtatg gtgtgtgata tggattgttt ttaggcacgc aaagctttta aaccatagta
     5041 cctttgcttg cttgaactgg ttttttattt aaacatggta catttatttg aaccttaacc
     5101 tttgggcctt tgcgagaaat acgttctaca tgttaagtgt atttgtttca cgcgaaggcg
     5161 cggagatttt atgtttgtat aatgtgtgat atggattgtt tttgagctcg cggagtttct
     5221 aaaccaaggc aacattgctt tctagaatag attttttatt taaaccttaa cctttgggcc
     5281 ccgatagcta taggggttgc tataactgca tacaaacatt aactttgtat catgcaaaac
     5341 aaaactatcg ctattggctg cgatcatgca ggctttcctt ataaagaagg tatcatcaat
     5401 tacctgacct ctaatggata taccgttaaa gactgtggca catcctcact cgattcagta
     5461 gattatcctg attacgtgca tccacttgcc caaagtattg agtcaggtga atcggccatg
     5521 ggcgttttaa tttgcggaag tggaaacggc gtatgtataa cagccaataa acaccaacaa
     5581 attcgcgctg ccatggtttg gcgtgaagat atttcagctt tatcgcgaca acataacaat
     5641 gccaatgtaa tttgtattcc tgcccgtttt attactttag aggaagcagt caattttgtg
     5701 catgtatttt tacataccga atttgaaggt ggcagacatg caacacgcgt taataaaatc
     5761 actcaatttt aattccattt ttttgatgaa atattctgca ttatttttcg cattgcttat
     5821 tgcaaccaat tgtgtttttg ctcaaaccga agaccttgga ataaaatatg ccaatactat
     5881 taccgcttcg gaattggaaa atcatcttac cattttcgcc agcgattatt acgaaggtcg
     5941 tgaaacagga acaagaggtt taagcagagc tgctgattat ttgtcaaaac aatttaccct
     6001 atccggcgtg cctccattgc aagctaacgg tggatatttt cgcgattatc cgctcatcca
     6061 atatcagtgg acaaattcta caatatcgtc atataaaaat ttgtttacca taatgaccga
     6121 tttttatgga tatgccggcg caaataattc tttcgcctat accgctaatg acatcgtatt
     6181 ccttggttat ggtattgatg acagcatgta tagcgattat aaaaatgttg acgtaaaggg
     6241 aaaaattgta ttaattgctg ccggcgaacc tatggttaac ggaaagtcgg taattaccgg
     6301 ttccgattca ttaagtgcct ggtcgaaaga ctggcgtaaa aaagctgctg ctgctacatc
     6361 aaacggtgta atgtgtttat tgacaattga ccctaagtta gcagaggtat taagtaatcc
     6421 gcaatggaaa aactttttgg aaggcagtat actcaaacta caatctgaat ataaacaacc
     6481 tgaatacacc aacaatttat ttattagtca aaacatggca gataaattat tgggaaaaaa
     6541 gaaaaaattc attcaaaaaa ctattgataa aatttccact accggcaagc cggctaattt
     6601 tttagtcaaa aatactatag tatttaatgt tgtaaaaaca gaaaatacta tttatgcaga
     6661 taacgtgttg gcgtttgtgg aaggaacaga tttgaaggaa gaagtaattg ttgtatctgc
     6721 ccattatgac catctaggaa aaaaagatac cgttgtatat aatggagcag atgacgatgg
     6781 ttctggcact attgctttat tagaaatttc ggaggctttg gcgaaagcga aatcaaatgg
     6841 tgagggacca cgacgcagcg ttttgtttat agccttctcc ggtgaggaaa aaggtttatt
     6901 gggttccaaa gcatataccg ataatccggc cattccattt gaaaatactg tcgctaattt
     6961 aaatattgat atgattggga gggttgatga aaagcatact gctacaccaa attatgtata
     7021 tattatcggc tccaattttt taagcacagc tttacatagt attaatgaag ctgcagcaaa
     7081 aaattatact aatattgaat tagattataa atataattct accactgacc ccaacagata
     7141 ctattacaga agcgaccatt ataattttgc taaaaacaat attccggtta ttttttactt
     7201 caacggcaca catgaagatt atcaccagcc aactgatgat gtagaaaaaa ttaatttccc
     7261 ggtatatgaa gcacgttgtc gcttagtatt ccatacctta tggattttag ctaatcagga
     7321 tgcaagaatt aaagtggatg ttgcgcagga agattgaaat tgttactttc atgtcgcaaa
     7381 acaatcctga tgacaaaact gattgccctt tttacttttt tatttatatc tatagctgca
     7441 ttcggtcaaa cggtttatac cgttgaaact attcccgacc ctaaacaaac cggtggggga
     7501 tatgtgagta atccggatgg ggtaattgat gcctcaacgg tacaaaatat taatgcagtt
     7561 ttggcggcgt tagaagattc tactactatt caggttgcag ttgttgtagt gaattcaatt
     7621 ggcgatgaag taccgggtga tttcagaaca aaattatttc gacattgggg aattggtcag
     7681 gccgataata ataatggttt actcatttta ttagtaatgg accagcgccg tatggaattt
     7741 gaagttggtt atggattaga aggtatttta acggatatca tgtgcgtgcg tattcagcaa
     7801 acttatatgg tgccattggc aaaagaagga cgcatgagcg aagcagtttt agctggtgtt
     7861 atgcaggtaa atcaaatatt aaccgacccg caatatcgcg atgaagttta tgctgacagt
     7921 ttaaacagtt cgtcaaataa tgcctggtgg aaagacccgg tttcgggaat gggattaggg
     7981 gtaattggtg gaatttatgc actaattgcc tatgccattt ttgccaatag aaaatcatca
     8041 ttaaaaaaag ccccttccta tgtgaaaaat aattattcag atgcttacac taaaacgaaa
     8101 tttggattgt tgaatattgg cgcaccggca ggtttgttgg cttggcagga aattgaaggc
     8161 tcattgagga tatttgaatt tgcagcaatt ttatatggca tttttttaat tattctttta
     8221 gaaaaacggt ttaggttaaa taaatacata ttaaaagaag gcaatacctt agagccacag
     8281 gaaacatata atctgttttc aaaatcgcat agcaatggat ggttagctgc aaccatattt
     8341 tttcctttac cttttgtatt gtacaatttg tttaacaaag gcagaatgaa caaattgcgc
     8401 aacacgccac caattgcaga cagtggaata gccatggtta aaatggatga aaaagaagat
     8461 gatggatttt taaaagcttt tcaaattaaa gaagaagaat tgcgttctgt cgattatgat
     8521 gtttggattg aaaacgcaac gcatcaggta aaaatttatc gttttgaaaa ttataacagc
     8581 cgatataaac cttgtccaaa ttgccagtcc attacatttt taatgacaaa aaatgaaacc
     8641 ttagtttccc cttcgtatac ttcttcagga accggacaaa aaacatatac ttgtaaag
//
LOCUS       DAORKW010000089         8685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_213_length_8685_cov_4.712399, whole genome shotgun sequence.
ACCESSION   DAORKW010000089 DAORKW010000000
VERSION     DAORKW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213_length_8685_cov_4.712399"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLB46_05295"
     CDS             complement(<1..494)
                     /locus_tag="PLB46_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75962.1"
                     /translation="MRRIIVILTVILMAKTGLYGQLMDNQVLFTPKDTLRGALRPERT
                     CFDVTYYNLTVDVNIGSKSIKGSNDIHFTAVEDFTILQIDLFQNLNITSITWKGKILE
                     FKRQFDAVFVQFPSTIKKGDKEIITIAYEGYPQIAKNAPWDGGFSFDTDINGNPWVGI
                     SCEG"
     gene            894..1301
                     /locus_tag="PLB46_05300"
     CDS             894..1301
                     /locus_tag="PLB46_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75963.1"
                     /translation="MKIKLLALLPISVSAIVIFAFSGCTSLPGDGGTSTITGKIYTEL
                     YNESGTLYQEYYSPDEKVFIVYGDNAAFDDEVSTAYDGTYKFEYLRKGNYTIYVYSDC
                     LLCPGGTEAKIVEVEIKDNNSVVELADIIIEKR"
     gene            1302..2054
                     /locus_tag="PLB46_05305"
     CDS             1302..2054
                     /locus_tag="PLB46_05305"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRF75964.1"
                     /translation="MSSLPEILNNFSPITLAEMENVELMNRKDMKYFFDRNKLPEILE
                     QMSEDYRVLDINGTRLFNYRNIYFDTKDFFFYTQHHNKRVNRYKIRIRQYVESNINFL
                     EVKFKTNTKRTLKSRIKIPEFTEVFSDKAKEFILQHTPFNPEDLVPTLRNDFKRFTLV
                     SKKLNERATIDTDLELEVPGNKRHFSNLCIAELKIDGSSSGSKFKVVMRDNLCPEVRI
                     SKYSVGAAFMYDELKQNNFKFKKLQINRIENA"
     gene            2047..2733
                     /locus_tag="PLB46_05310"
     CDS             2047..2733
                     /locus_tag="PLB46_05310"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRF75965.1"
                     /translation="MLNLLLQDGDESVRFIDLKLFDAEDFTDLILRSLFNFIIVFILV
                     KFIYRSDKKNKNYAFSFYLFSMIIFFLCYLLSGIKLEMGFAFGLFAIFSILRYRTISI
                     PMKEMTYLFMVIAIAVINALTTKKVSFMELLFTNLSLIAASFFLERLWYKEGLSEQTI
                     EYEKIENIKPERRAILIADLKERTGLDIRSAEILTTDFLRDMARVRVYYKASAQSNDD
                     DSAMDDDDDD"
     gene            2756..3364
                     /locus_tag="PLB46_05315"
     CDS             2756..3364
                     /locus_tag="PLB46_05315"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HRF75966.1"
                     /translation="MCCVCFSSVLFAQHTDGGAWISVGVSHEPVNDFTLEVNEEIRYN
                     ISVEELYQINTNISVDYKLSKKFKTGVDYRYSVRDGRNTNRFGWGISYKEGMQDFDLS
                     LRSKLQYSPVPDGAEGTSWRNKAALSYQINKDFSPFVSGELFYSMSNEIDQLDNYRVE
                     AGLDYGPNKHHDFTISWLYDVEFNVNNPDKMQVITFGYKHSF"
     gene            3364..3699
                     /locus_tag="PLB46_05320"
     CDS             3364..3699
                     /locus_tag="PLB46_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75967.1"
                     /translation="MKYCFGLFFTMLVVVKQSDAQFVADKAIPSNLELNTDVEVVNLT
                     ITTKTDYTEQNSNHSKQMKWLNSAILHRDLNNYYHTVTLPAYVQGKFCDFEDAINKGN
                     LFRIDFSVK"
     gene            3776..4684
                     /gene="hemF"
                     /locus_tag="PLB46_05325"
     CDS             3776..4684
                     /gene="hemF"
                     /locus_tag="PLB46_05325"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HRF75968.1"
                     /translation="MKTLDRFVITDWFMHLQDRICHAIEKTDGKGVFEEELWQRPGGG
                     GGRTRIIQNANIIEKGGVNFSQVHGVLPEKIAHQLQVKGHDFFATGVSIVMHPFSPLV
                     PIIHMNVRYFEVSSGEQWFGGGIDLTPIYVDPKQAQYFHQQIKHTCDLHDHTYYETFK
                     KWADDYFFIKHRNETRGVGGVFFDRLGATDNISLYQRFEFVKDIGNIFAPIYTKFMEE
                     NKDISYGKGEKQFQAIRRGRYVEFNLVYDKGTKFGLDTDGRTESILMSMPPEANWLYN
                     YQPLAGSKEAATLAFLRKDVDWISIN"
     gene            complement(4701..5102)
                     /locus_tag="PLB46_05330"
     CDS             complement(4701..5102)
                     /locus_tag="PLB46_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75969.1"
                     /translation="MASCVKPPDYPIEPRIEFVSMNANTFDELDEDSLSVVIYFEDGD
                     GDLGSDDSVNMYWEDSRVPGYQVPFKIPLIAIQGNSKAISGTITTFYPISFCINDDDP
                     IDSFYYKIFIVDRAGHESNVDSTDMIFLNCN"
     gene            5190..6179
                     /locus_tag="PLB46_05335"
     CDS             5190..6179
                     /locus_tag="PLB46_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HRF75970.1"
                     /translation="MQNIAGLIQNLFDGDEQSIHSKTLAIFRFQAENCAVYKEFLQYL
                     DIQPATVTSIEAIPFLPITLFKSRTVLIDGCQPDFYFESSGTTQMERSKHFVPDLTLY
                     EKSFRNSFHLFYGDVKQYCIAALLPSYLERKNASLVYMCNDLITSSQQTGSGFFLDDF
                     AQLAELIRYNETNGIRTLLIGVSFALMDFADSFPMRLQHTTIMETGGMKGKRPELTRE
                     ALHAYLNKSFQTSTIHSEYGMTELLSQAYSNGNGIFQTPPWMKILIRDTEDPFTILGY
                     NKTGCINVIDLANIYSCSFIATQDLGKLHENGSFEVLGRFDKSDVRGCNLMVE"
     gene            complement(6188..7837)
                     /gene="pgi"
                     /locus_tag="PLB46_05340"
     CDS             complement(6188..7837)
                     /gene="pgi"
                     /locus_tag="PLB46_05340"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRF75971.1"
                     /translation="MATYPSFYQTRAYEKLKNSFLQLQNGHMRDMFQADANRFDKMHV
                     NFENMLFDYAKNRLTTEVIQQLSELAEELELQNQMLAMFEGEPINKTENRPVLHTALR
                     NFSGNPVIVNGENVMPKINAVLKKMKSFSDGVIDGSIKGYTNHYFTDIVNIGIGGSDL
                     GPVMVTEALKHYKVPHLQVHFVSNVDGTHITEVLNTLNPETTLFLIASKTFTTQETMA
                     NAHSAREWFLQTAHDETFIAKHFVALSTAEKEVVQFGIAKENIFEFWDWVGGRYSLWS
                     AIGLSICLAIGYDNFEQLLKGGYAMDQHFLKEPFHQNIPVLMALIGFWYIEFFGWHTQ
                     AIIPYDQYLHRFTAYLQQADMESNGKYIDRNGKRVNHATGPIIWGEPGTNGQHAFFQL
                     MHQGTSIIPADFIAPAKSLNKIGNHHQLLLSNFFAQTEAFMKGKTAEEVENELANKGF
                     TTEQIKYHTPFRVFEGNRPTNSIMFDELNPYNLGKLISAYEHKIFVQGVLWNVFSYDQ
                     WGVELGKKLASAILPELTSTTTITTHDNSTNGLINWTKSKQ"
     gene            complement(7843..>8685)
                     /locus_tag="PLB46_05345"
     CDS             complement(7843..>8685)
                     /locus_tag="PLB46_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75972.1"
                     /translation="NGRMKLIADSGSTKTDWALIGEQAVLFTSSGLNPNILKDEEIVF
                     ALNNIKEFTDNKSNISEVFFYGAGCGSPANCVRLEAIFKQFFPSAIIQIKTDIEGAAF
                     AADNGKPGIVAILGTGSNSCYFDGDNVFGSDFSLGYILGDEGSGSYFGKRLLRDFCYQ
                     LLPDDLHIAFENTYAVNRAAIIEKVYRQPAPNEFIASFLPFYKEHLQHPYCTYFINFG
                     LEEFIKIYIWRFSNYKSVDVHFVGSVASHFEEQLRKVCANNSIHIGKIIAAPIQGLIQ
                     YHNK"
BASE COUNT         2818 a         1478 c         1585 g         2804 t
ORIGIN      
        1 aagccttcgc aggaaatgcc cacccatgga ttaccattaa tatctgtatc aaaggaaaaa
       61 ccaccatccc agggagcatt ttttgctatt tgaggatacc cctcataagc tatggttatg
      121 atttctttat cacctttttt gatggtggat gggaattgca caaaaactgc atcaaattgg
      181 cgtttaaatt ccagtatttt acccttccat gtaattgatg tgatgtttaa attttggaag
      241 aggtcgattt gtaaaattgt aaaatcttca acagcagtga aatggatatc atttgagcct
      301 ttaatggact ttgatcctat atttacatct accgtaaggt tataataggt aacatcaaaa
      361 caagttcgtt ccggtcgcaa tgcacctcgt agtgtgtcct ttggcgtaaa caacacctga
      421 ttgtccatta attggccata caacccggtt tttgccatca aaatgacagt cagtattaca
      481 ataattctcc tcataggttt tggttcttga tacaatgata aatggttttt aatcgccaaa
      541 ccacgtttgt taacccgttt taacgaattt tatcactaaa atcggatttt ttgacacgtt
      601 taacctgtag gtatacaaac cttacaccaa attgaatcca tattgtgtaa cgcctgctca
      661 ataatactgt cattctctgg taaaactgtc taaccagctg ggtttcaatg catagcattc
      721 gaataaaaaa atgttacatt tgggaaaaat taatacttta aaccagatag taattaaact
      781 ttgaatgttg aaacattaat ttcaaatgct cgtaaaagcc gttaatttta cggaggtaac
      841 aattaattta catctagtta aactttaatt aacccaatct gtccaattac gatatgaaaa
      901 taaaactcct cgctttattg cctataagtg tttcggcaat tgtaattttt gcatttagcg
      961 gctgcaccag tttacccgga gatggaggaa cttctacaat aaccggcaaa atatacacag
     1021 agttatataa tgaatcaggc accttgtatc aggaatatta ttcgccggat gaaaaagtat
     1081 ttattgtgta tggcgataat gcggcatttg atgatgaggt aagtactgct tatgatggca
     1141 cttacaaatt tgaatattta cgcaaaggta actataccat ttatgtgtat tcagattgtt
     1201 tactttgtcc gggtggcact gaggcgaaaa ttgtagaagt agaaataaag gacaacaatt
     1261 ctgttgttga attagcggac attattatag aaaaaagata agtgagtagt ttacccgaaa
     1321 tactaaacaa cttctctcct attacattag ccgaaatgga gaatgtagag ttaatgaatc
     1381 gaaaagatat gaagtatttt ttcgacagaa ataaactacc ggagatactt gaacaaatgt
     1441 cggaggacta cagagtatta gatatcaacg gcactcgttt atttaattac cgaaacattt
     1501 attttgatac taaagatttt ttcttctata ctcagcatca taataaacga gtaaatcgat
     1561 ataaaatccg cattagacaa tatgtagaaa gtaatatcaa ttttttagaa gtaaagttta
     1621 aaacgaatac caaacgcact ttaaaatcaa ggattaaaat accggaattt acagaagtgt
     1681 tttcagacaa ggcgaaagaa tttattttac aacacacgcc atttaacccc gaagatttag
     1741 tgccaacttt gcgcaacgac tttaaacgtt ttacgctggt aagtaaaaag ctcaacgagc
     1801 gggcaacaat tgatactgat ttagaattag aagtacccgg taacaaaagg cattttagta
     1861 acttatgtat tgccgagctc aaaattgacg gttcatcatc aggttctaaa ttcaaagtgg
     1921 taatgcgcga caacttatgt ccggaagtgc gcataagtaa gtatagtgtg ggcgcagctt
     1981 ttatgtatga tgaattaaaa cagaacaact tcaaatttaa aaaactacaa ataaacagaa
     2041 tcgaaaatgc ttaatttact cctacaagac ggtgatgaat cggtgcgatt tatcgacttg
     2101 aaactatttg atgcagagga tttcaccgac ctcatattgc gctctttatt taatttcatt
     2161 attgtattta ttctggtaaa atttatttat cgaagcgaca aaaagaataa aaactatgca
     2221 tttagtttct atttatttag tatgattatt ttctttttat gttatttatt atcgggtatt
     2281 aaacttgaaa tgggtttcgc ttttggtttg ttcgctatat tttcgattct gcgttaccga
     2341 accattagta ttccgatgaa ggagatgact tatttattta tggtaattgc aattgcggta
     2401 atcaacgcat taacaaccaa aaaagtaagt tttatggaat tgttattcac taatctttca
     2461 cttattgctg cttcattttt cttagagcgc ttatggtata aagaaggttt gagtgagcaa
     2521 actatagaat acgaaaaaat agaaaacatt aaacctgaac gaagagcaat tttgattgcc
     2581 gatttaaaag aaagaacggg attggatata cgttctgcag agatattaac cacagacttt
     2641 ttacgtgata tggcccgcgt tagggtttat tataaagcca gtgcacaatc gaacgacgat
     2701 gatagtgcta tggatgacga tgacgatgat taaaaaaata tccatattag tttttttgtg
     2761 ttgtgtatgc ttttcgagtg tgctgtttgc tcagcataca gatggaggag catggataag
     2821 tgtgggtgtt agccatgagc ctgtcaatga ctttacttta gaagtaaatg aggaaattcg
     2881 atataatatt tctgtggagg aattatatca aatcaatacc aatatttcag ttgattataa
     2941 gttatctaaa aaatttaaaa ccggagttga ctatcgttat tcggttagag acggtcgaaa
     3001 taccaatcgt tttggatggg gaatcagtta caaagaaggc atgcaggatt tcgatttaag
     3061 tttgcgttct aagttgcaat acagtcccgt acctgatggc gcagaaggaa catcctggag
     3121 aaataaagct gcattatcgt atcaaattaa taaagacttt agtccgtttg tttccggcga
     3181 gttgttttac agtatgagca atgaaattga ccaattagat aattatcgag ttgaggcagg
     3241 attggattat gggcccaaca aacatcacga tttcactatt tcgtggttat acgatgttga
     3301 gtttaatgtg aacaatccgg ataaaatgca ggttattaca tttggataca aacactcatt
     3361 ttaatgaaat attgttttgg gttatttttt acaatgctgg ttgttgttaa acaatcagat
     3421 gcccaatttg tggctgataa ggcaatacca tcaaaccttg agttgaatac cgatgtcgaa
     3481 gttgttaatt tgaccattac aacaaaaact gactacacag agcaaaattc caatcattca
     3541 aaacaaatga aatggttgaa ctcagcaata ttgcacagag atctaaataa ttattatcac
     3601 actgtcactt tacctgctta tgttcaggga aaattttgtg attttgaaga tgctataaac
     3661 aagggtaacc tttttcgtat cgactttagt gttaaataag gttgatgacc ttaatcattt
     3721 ttagagtagc ccgtaatggt taactttgtt acattcatta ataaatgctg caattatgaa
     3781 aactttagac cgctttgtga ttaccgattg gtttatgcat ttgcaggata gaatctgcca
     3841 tgctattgaa aaaacagatg gtaaaggagt ttttgaagaa gagctgtggc agcgtcccgg
     3901 tggaggtggc ggaagaacac gtattattca aaatgccaat attatagaaa aaggcggcgt
     3961 caatttttca caagtgcatg gtgttttacc tgaaaaaatt gcccatcaat tacaggtaaa
     4021 agggcatgat ttttttgcaa cgggagtatc tattgttatg catcccttta gcccgttggt
     4081 gccaataatt cacatgaatg tcaggtattt tgaggtaagt tcaggtgagc agtggtttgg
     4141 aggtggtatc gatttaacac caatatatgt tgatccgaag caagctcaat attttcatca
     4201 gcaaataaaa catacctgcg atttgcatga tcatacctac tacgaaacat ttaaaaaatg
     4261 ggcggatgat tatttcttta tcaaacatag aaatgaaaca cgtggtgttg gcggtgtgtt
     4321 tttcgacaga ttaggtgcta cagataacat ctcgctttat cagcgatttg aatttgtaaa
     4381 agatatcgga aatattttcg ctcctattta caccaaattt atggaggaaa ataaagacat
     4441 ttcttatggt aagggtgaaa aacaatttca ggcaatcaga agaggaagat atgttgaatt
     4501 taatctggtt tatgataagg gaacaaaatt tggtttagac accgatggca gaactgagtc
     4561 aattttaatg agtatgccac ctgaagccaa ttggttatac aattatcaac ccttagccgg
     4621 tagtaaagaa gctgcaacgc tagctttttt gcgtaaagat gtagactgga ttagtattaa
     4681 ttaaaaactt agtcctgaag ttagttgcaa tttaaaaata tcatatcggt tgagtcgaca
     4741 ttactctcat ggcctgctct gtccacaata aatattttat agtagaaact gtcgatgggg
     4801 tcatcgtcgt tgatgcagaa tgagatggga taaaatgtag taattgttcc tgaaatggct
     4861 ttactgtttc cttgaatagc aatcagcggt attttaaaag gcacctggta gcccggaact
     4921 ctactgtctt cccaatacat attcacgctg tcatcgctgc ctaaatcacc atcaccatct
     4981 tcaaaataga taacaacgct aagactgtct tcatccaatt catcgaaggt attggcgttc
     5041 atactgacaa attcgatacg tggttctata ggataatcag gaggcttaac acaggaagcc
     5101 acaaagaaaa tgattatcag aattaggaag ctgtatcgat gcatcatatt tttacatttc
     5161 aaaattacac ataaattaac tcaaaaccaa tgcaaaatat tgccgggctt attcaaaatt
     5221 tgtttgatgg agatgaacaa tctatccaca gcaaaaccct ggccatattt cgttttcagg
     5281 ctgaaaattg tgctgtttac aaggaatttt tacagtattt agacattcag cctgctactg
     5341 tcacgagcat tgaagcaatt ccctttttac ccatcacctt gtttaaaagt cgtacggtat
     5401 taattgatgg ttgccaaccg gatttttatt ttgaaagcag cggaacaaca caaatggaac
     5461 gcagtaaaca ctttgttccg gatttgacat tatatgaaaa aagttttcga aacagttttc
     5521 atctgtttta tggagatgta aaacagtact gtattgccgc attacttccc tcctatttgg
     5581 agcgtaaaaa tgcatctctt gtttatatgt gtaacgattt aattacctca tcacaacaaa
     5641 ccggaagtgg attttttctg gatgattttg cgcaactagc agaacttatc cgctataatg
     5701 aaaccaatgg aattcggact ttactgattg gtgtttcatt tgcgttaatg gattttgctg
     5761 acagtttccc aatgcgctta caacatacca ctattatgga aaccggaggt atgaaaggca
     5821 aacgaccgga gttaacgcgt gaggcattac acgcctattt aaataaatcc tttcaaacat
     5881 caaccataca ttctgaatat ggcatgactg aattattatc acaggcgtat tcaaatggta
     5941 atggcatttt tcaaacacct ccatggatga aaattctgat tcgcgataca gaagaccctt
     6001 tcacaatttt agggtataat aaaacaggtt gtattaatgt aattgattta gccaatattt
     6061 attcctgtag ttttattgct acacaagatt taggaaaatt acatgaaaac ggttcatttg
     6121 aagttttggg ccgtttcgat aaaagtgatg tacgcggatg taatttaatg gtggaataac
     6181 ctattcatta ctgtttagat tttgtccaat tgattaaacc attggtgctg ttatcgtgag
     6241 tagtaattgt tgttgtagat gttaattccg gtaaaatagc agaagccaat ttttttccta
     6301 attcaactcc ccattgatca taactaaata cattccataa aacaccttgc acaaatattt
     6361 tatgttcgta tgcggaaatt aatttaccga ggttataagg atttaattca tcgaacataa
     6421 tactgttagt aggtctgttt ccttcaaaca cacgaaatgg tgtatgatat ttaatttgct
     6481 cagtagtaaa tcctttattg gccaattcat tttcaacttc ttcagcagtt ttacctttca
     6541 taaaggcttc ggtttgggca aaaaagttgc tgagtaataa ttgatgatgg ttgcctattt
     6601 tatttaatga tttagcaggt gcaataaaat ctgccggaat tatgctagtg ccctgatgca
     6661 ttaattgaaa aaatgcatgc tgaccgttag tgccgggttc tccccaaata attggaccag
     6721 ttgcatggtt tacgcgttta ccattgcggt cgatgtattt gccgttactt tccatatctg
     6781 cttgctgcag ataggctgta aatcgatgca aatattggtc gtaaggaata attgcttgtg
     6841 tatgccatcc aaaaaattcg atataccaaa aaccaattaa tgccatcaac accggaatat
     6901 tttgatggaa tggttccttc aaaaaatgtt ggtccatggc atatccgcct tttaataatt
     6961 gctcaaaatt atcgtaacca atggcgaggc aaatagataa accgattgca ctccataaag
     7021 aataacgtcc tcctacccaa tcccaaaatt caaatatatt ttctttagca ataccaaatt
     7081 gtactacttc cttctctgca gttgaaagcg ctacaaagtg tttggcaata aatgtttcat
     7141 catgtgccgt ttgcaaaaac cattcgcgtg ctgaatgcgc atttgccatt gtttcctgcg
     7201 tagtgaaagt tttggatgca attaaaaata atgttgtttc aggatttaat gtatttaata
     7261 cttcagtaat atgtgtgcca tcaacattgg aaacaaagtg cacttgaaga tgtggcactt
     7321 tataatgttt gagtgcttct gtaaccatta caggtccaag gtcagaacct ccaataccaa
     7381 tattgacaat atcggtaaaa taatgattgg tgtatccttt aatgctgcca tcgataacac
     7441 catcggaaaa cgatttcatt ttttttaaaa ccgcatttat tttaggcatc acattttcac
     7501 catttactat aacagggtta cctgaaaaat tgcggagggc agtgtgtaat accggacggt
     7561 tttctgtttt attaatcggt tcgccttcaa acatggccag catttggttt tgtaattcca
     7621 gttcttctgc caattccgat agttgttgta ttacttcagt agtcaacctg tttttagcat
     7681 aatcaaacaa catattttcg aagttgacat gcattttatc aaaacgattt gcatctgcct
     7741 gaaacatgtc acgcatatgc ccattttgca attgcagaaa tgaatttttc agtttttcat
     7801 atgcacgtgt ttgataaaaa gatggatatg ttgccatgta atttacttat tgtgatactg
     7861 gattaaaccc tgaattggag cagcaattat tttacctata tgaatactat tattagcaca
     7921 aacctttcgc aattgttcct cgaagtgaga agctacagag ccgacaaaat ggacatccac
     7981 agatttgtaa ttactgaatc gccaaatata tattttaata aattcttcta aaccaaaatt
     8041 aataaaatag gtacaatatg gatgctgcag atgttcttta taaaatggta aaaatgaggc
     8101 tatgaattcg tttggggccg gctgacgata cactttttca ataattgcag ctctgtttac
     8161 cgcatatgta ttttcaaagg caatatgtaa atcatcaggt aataattgat aacaaaaatc
     8221 gcgtagtaat cgtttcccaa aataggagcc actgccttca tcacccaaaa tataacctaa
     8281 actaaaatca cttccaaaaa cattatcacc gtcgaaataa caggaattgg aacctgttcc
     8341 caaaatggct acaatgccgg gtttaccgtt atcggcggca aaagccgcgc cttcaatatc
     8401 agttttaatt tgaataattg cagatgggaa aaattgttta aatattgctt ctaaacgaac
     8461 acaatttgcc ggagaaccac aaccggctcc ataaaaaaac acttctgaaa tgtttgattt
     8521 attatcagta aattctttga tattattaag tgcaaaaaca atttcttcat cctttaatat
     8581 attaggattt agtccggatg aagtaaaaag taccgcttgc tctcctatta gcgcccaatc
     8641 tgtttttgta gaaccgctat cagcaatcaa tttcatccta ccatt
//
LOCUS       DAORKW010000090         8683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_214_length_8683_cov_4.388271, whole genome shotgun sequence.
ACCESSION   DAORKW010000090 DAORKW010000000
VERSION     DAORKW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8683
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214_length_8683_cov_4.388271"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..509
                     /locus_tag="PLB46_05350"
     CDS             87..509
                     /locus_tag="PLB46_05350"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRF75973.1"
                     /translation="MKKWLFNANATYFNIGMLFLRIALGASFIYNHGFKKIADPSRWG
                     KLGAEMQVIHIDFAPAFWGFMAAFSEFFGAVFIILGLFTRLSAFMLAFTMFIAFMAGN
                     SPNDFNAYPFEMMLVFITIMIIGPGKLSIDAQISGFKK"
     gene            950..1840
                     /locus_tag="PLB46_05355"
     CDS             950..1840
                     /locus_tag="PLB46_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75974.1"
                     /translation="MRQTKYLLVLLLPLLSYSCSRDSVVHEDLIIDGNVAPPIDGVAE
                     ITLNNYINNLYIDLYGRAATEDEISDHVSYLKENNYAESARKVIVEELLNNWDYYKNF
                     NLLTSQKLLVNLDSFSLKSQIDYWVYIIDLLNATGMETEAYYYTYENNRLLLLSTAHV
                     DLYSGVIGIKEYYRRYIDNYFYDQVNMGSENFVVSCFTNLLHRYPTDDEKLQGVNMCD
                     GGSGHLFLQDGSSKGDFIESFVNSLEFYQGQVIEVYQTFLARLPESVEMNEYSLQMQE
                     EDNFNNVKVDILISEEYAGF"
     gene            1878..2366
                     /locus_tag="PLB46_05360"
     CDS             1878..2366
                     /locus_tag="PLB46_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75975.1"
                     /translation="MSFTTTFVACKKNIVEEVIYDNIIYQVDTVPVYQNNSEKDRLKT
                     PLQYISSLYSNLYYSSIPSNLLDNLVIYRLSIGDKTLVNEMILNAMLKDPVVLANIPK
                     NYEMRNDVDAFVKECYLRFYLRLPTEYEKYGLVNIIEADDDIDVVDVYRSFLLSNEYM
                     FY"
     gene            2388..3716
                     /locus_tag="PLB46_05365"
     CDS             2388..3716
                     /locus_tag="PLB46_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75976.1"
                     /translation="MNRRKFIKNSLLTTAGMISAPYILPAGTLFRSSGARLANHVVLV
                     VFGGGIRNQESVEQQYLINQGAGPTGNIMRNMLNGASPASNLVYDIWDPVTPSPLVTK
                     GTLFKHMKYASGPTGHFNGHTAVITGNYTPDSLNLYANPATPTLFEYYRKHNDPQKTA
                     LNAWWMSIDLGPYVNLNYSLDKLYGSQYGANYLSPQTTFGGLGIDYFSTLNTMHPEEL
                     ARLNSIKSFLNSNFNRSVTDIPGINNTEDDKQAIQDFVLKVIKGEIPIEWPLPPGVGF
                     DQLSGDLSNVAISWKVLQQFHPELMVINTTNLDICHTNFSEYISYLHRADYGVGWLWQ
                     KIQSDPVLANDTIMICIPEHGRNQVANAIPDANGLFAYDHTGDDNSRSVFSLIVGPPS
                     VVKQNLVVGSEEDPIGETIDIVPTIAHILGFKDSIPSGYLPGNVLEQAFV"
     gene            3727..4695
                     /locus_tag="PLB46_05370"
     CDS             3727..4695
                     /locus_tag="PLB46_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75977.1"
                     /translation="MKILIPIASVLAMIIVSCQREITRIEPEIPFNPFDTIDYSDGIL
                     DDVPIDSASFLGLHKYIFSKTCAVPGCHDGTFEPDFRTVQSAYNTLLYAPVIKNDAAG
                     TFTYRVIPGDTALSWLHERITTEDEVLGRMPLYDTLYPEEREKITQWILNGAPDVFNQ
                     SPTLADYQPVTYGFIAYENDTNGMRLDTIRADFFSPVQVPANSNVEFWFGFYDTDENG
                     VFQMPSSLQENIIRVSQDMFMNTGYTEYTFELEPNFEPFYGPTYYDPLVEVTYWHHFT
                     IDTDDFEPGQVYFMRAYIQDNAHDFLTEIPDDSQLYIMGYFSFVVE"
     gene            4692..5147
                     /locus_tag="PLB46_05375"
     CDS             4692..5147
                     /locus_tag="PLB46_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF75978.1"
                     /translation="MIKNTILAVAATLVIGCGKEDDIINPPMSDVPAIELINVSPSTV
                     TALGDSIVFIIHYEDGNGDLGFNAPDSMSLYITDTRIPLTESFFVPLLAPEGADIAIQ
                     GELEVVLNNTILVDPEATSETVQFEIQLRDRSGNYSNVLVAPTITVLPE"
     gene            5304..6530
                     /gene="hemA"
                     /locus_tag="PLB46_05380"
     CDS             5304..6530
                     /gene="hemA"
                     /locus_tag="PLB46_05380"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRF75979.1"
                     /translation="MNQYKVLTVTHKHVPVEQLKHYIIDSDKSSPEFGHKLHFIKQNM
                     HLDELMYLNTCNRVTYFFTTKNQLGSGFTANFFKTINPAFEACNAVNSNMYEGVEALE
                     HLCEVSASLDSMVVGEREIIRQLRVAYDDSNNLKLTGDDIRLAMKMLIPAAKKIYSET
                     KIAEKPISVVSLAALQLKALKLSPEAKILLIGAGETITNMSTYLVDMGFKNFIVFNRT
                     LANAEKLAQAINGKAYPLAELENYTDGFDVIITSTGSAKKEITAPVYQKLVGSNKDKK
                     VIIDLAIPLDTDESIITDFNVTYIKIDTLRAIAQKNLSSRKAEVLKAKHLVHEFVKEF
                     QAVYVERMVEKAHSIIPIKLNEIRTKAVEEVYAKEMASLDDNAKEVVDKLLDYMEKKY
                     LALTMAASKDAMRHKH"
     gene            6545..8098
                     /gene="hemC"
                     /locus_tag="PLB46_05385"
     CDS             6545..8098
                     /gene="hemC"
                     /locus_tag="PLB46_05385"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRF75980.1"
                     /translation="MQETIIIGSRGSDLALWQAHFLQKTLLEQGYRSEIKIIKTQGDK
                     IQHISLEKLEGKGFFTKEIEDALLMGEIDVAVHSHKDLPTSITPGLTIAAVSHREDPA
                     ELLLIRNEKVDELNEFNLTTNAIVGTSSSRRNVQMRFFRPDLQFADIRGNVPTRINKL
                     RNGDYDAILLAFAGVYRLQLDISDLTAVKIPSHKLIPSPAQGVLAFQCREKDEHMIGI
                     LEKINQPDVADTIYIERKVMNLFNGGCHMPLGVYCRKHNEQYQVWAAQTADRLLPLKR
                     LFVEGNNADQLATHIFNTLQQQQQKSIFISTDLNEDALYLQVLRDKGFEVIGKSCVHM
                     EFIPFQFSEATDWVFFTSKNGVDYFFNSVKSISPKIKFAAIGEATAKQINQHGYAVEF
                     VGEGTIAQIAAQFKLLAAGEDVVFAVAENRQTALQSHIAQFALTTIIPVYNNTPIAIE
                     PVQADIYVFTSPLNVDGFLLNNNIPCTAKIVAIGATTQQHLIDKGYTQVAVPPFHNLF
                     SVAELICGW"
     gene            complement(8103..>8683)
                     /locus_tag="PLB46_05390"
     CDS             complement(8103..>8683)
                     /locus_tag="PLB46_05390"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HRF75981.1"
                     /translation="TGPTYAIQGNILLGPEILDNMEANFLATEGNLGCKLMSALQGAK
                     VPGADTRCAEFDISALSSFLRVARPTDEPGSFLIDLRVFDVEGDSIDPIDSLQTLYDA
                     IGGCNFNGVSNLPLIAPTIYPQPTNSSVIFQLSQVYDNITISSINGEIVYTKSIAGQQ
                     QLIINCENWIPGIYLYKLITKEGNQTGKIIVE"
BASE COUNT         2782 a         1469 c         1668 g         2764 t
ORIGIN      
        1 tacacaacaa attcatttgg tatttcgtta gctaatactt ttggatttgg tattacttat
       61 cattttataa aagattaaac tgaaaaatga aaaaatggct ctttaatgca aatgctacct
      121 attttaatat aggcatgttg tttttacgaa tagcattagg tgcatctttt atttataatc
      181 acggatttaa aaaaattgcc gacccttccc gctggggaaa attaggtgct gaaatgcagg
      241 taatccatat tgattttgca ccggcatttt ggggttttat ggctgccttt tccgaatttt
      301 tcggagcggt gtttatcata ttgggattgt ttacgcgttt atctgcattc atgttagcat
      361 ttacgatgtt tattgcattt atggccggta attcccccaa cgattttaat gcttatccat
      421 ttgaaatgat gcttgtgttt attaccatca tgataatcgg tcccggaaaa ttgagtattg
      481 acgcacagat ttccggcttt aaaaagtgat gctgtgtact actgtgtaca agtttgttga
      541 attttattaa aacagttcgc tgtaaccctt gctgcaatgc acttttaaac cattgagtac
      601 aaaattgtac gctgtgtaca aagttgttga atacagcact tgtacaacgt acagaaacaa
      661 tctacttttg acttatcaaa taaaacaaaa caagttatcg ttagtaaaaa atcaaaagca
      721 tattaacggt aatatataaa atataccacg atgcttttgc tgtgtttcgg ttcaagaaac
      781 agcacaagta tgaaaagaga acggctccag gtgagccgtt tttttatttt tggcagattt
      841 tttgcttcta catacttaaa ttcccttttt aacgtttttt tgtatatttg gtctagctcc
      901 tgcaacccac acactgttag ttacacattg ctaatattaa ttatagagca tgcgccaaac
      961 aaagtatctg ttagttttac tacttccatt actgagctac tcttgttcaa gggatagtgt
     1021 tgttcacgag gatttaatta ttgacggcaa tgttgctcct ccaattgatg gggtggccga
     1081 aattacgttg aacaattaca tcaacaattt atatatcgac ttatatggca gggccgcaac
     1141 tgaagatgaa atcagtgacc atgttagtta tctgaaagaa aataattatg cagagtctgc
     1201 acgtaaggtt attgttgagg aattgttaaa caattgggat tattacaaga attttaattt
     1261 gttaaccagc caaaaattac tggtaaacct cgatagcttt agcctaaaat cgcaaatcga
     1321 ttactgggta tatattattg atttgttgaa tgctaccggc atggaaacgg aagcatatta
     1381 ttatacttat gaaaacaaca ggttgttgtt attatctaca gcgcatgttg atttatatag
     1441 tggtgttatt ggtattaagg aatattatag gagatatatc gacaattatt tctacgacca
     1501 ggtaaatatg ggtagcgaaa attttgtggt atcctgtttt acgaatttat tacaccgtta
     1561 tcctactgat gacgaaaaat tgcaaggtgt aaatatgtgt gatggaggca gtggtcattt
     1621 gtttttacag gatggcagca gcaaaggtga tttcatcgaa agttttgtga attcactcga
     1681 gttttatcag gggcaggtga tagaggtgta tcaaacattt ttagcccgtt tacccgagag
     1741 tgtggagatg aatgagtata gtttacaaat gcaggaagag gataatttca acaatgtaaa
     1801 agtagacatt ttaatttctg aagaatatgc cggattttaa attcatcagc aggaccataa
     1861 ttacaggttt tgtaattatg agtttcacaa caacatttgt tgcttgtaaa aaaaatattg
     1921 tagaagaagt tatctacgac aatattattt atcaggtaga cactgttccc gtgtatcaaa
     1981 ataattcgga gaaggacagg ctgaaaacgc cgctgcaata tatttccagc ttatatagta
     2041 atctttatta ttccagtata ccttctaatt tactggataa tctggtgata taccgattat
     2101 ctattggaga taaaacattg gtaaatgaga tgatactcaa tgctatgttg aaagatcctg
     2161 ttgtgttagc aaatattccc aaaaattatg aaatgcgaaa cgatgtggat gcttttgtga
     2221 aagagtgtta tttgcgtttt tatttgagat taccaactga atatgaaaaa tacggattag
     2281 taaatattat tgaagcagat gatgatattg atgtggtgga tgtttaccgc tcgtttttat
     2341 taagtaacga atacatgttt tattaattac ccttcaaacc ggattgcatg aacagaagaa
     2401 aatttattaa aaactcattg ctcactacag cgggcatgat aagtgcacct tatatattac
     2461 ctgccggtac attatttcgg agttccggcg cgcgattagc caatcatgtg gtactggttg
     2521 tttttggcgg cggtattcgt aatcaggaat ctgttgaaca acaatacctc atcaatcagg
     2581 gagcagggcc taccggaaat attatgcgca acatgttaaa cggtgcttca cctgcatcta
     2641 atttagtgta tgatatttgg gacccggtaa caccatcacc attggttaca aaaggtactt
     2701 tgtttaaaca catgaaatat gcaagtggcc caacaggaca ttttaatggc cacacagcag
     2761 taataactgg taattataca ccagatagtt tgaatttata tgcaaatcct gcaacaccaa
     2821 ctttatttga atattatcgc aaacacaacg acccgcaaaa aactgcatta aatgcgtggt
     2881 ggatgtcgat tgatttaggc ccttatgtaa atttaaatta tagtctggac aaactgtatg
     2941 gttctcaata tggtgctaat tatttaagtc cccaaacaac atttggagga ctaggaatag
     3001 attatttttc tactttaaat acgatgcatc ctgaagagtt ggcgagatta aattctatta
     3061 aatcattttt aaatagcaat tttaatcgca gtgtaaccga tattcctgga attaataata
     3121 ctgaagatga taaacaggct attcaggatt ttgtgttgaa agtaattaaa ggagaaattc
     3181 caatcgaatg gcctttacct cctggcgttg gatttgatca gctttccggc gacttatcca
     3241 atgtggcaat ttcatggaag gtattacaac aattccatcc tgaattaatg gtaattaata
     3301 cgactaacct ggatatttgt cacaccaatt tcagtgaata tatttcttat ttacaccgcg
     3361 ctgattatgg tgtgggttgg ttatggcaaa aaatacaaag cgacccggta ttggctaatg
     3421 ataccataat gatttgtatt cctgaacacg gcagaaatca agttgccaat gccatacctg
     3481 atgcgaatgg attatttgct tacgaccata ctggtgatga taattcaagg tcggtattta
     3541 gtttaattgt tggaccacca tccgttgtta aacaaaattt agtggtagga agtgaggaag
     3601 acccaattgg tgaaacaatt gatattgttc caactattgc acatatttta ggatttaagg
     3661 atagtattcc ttcaggttat cttccaggta atgttttaga acaagctttt gtataatatg
     3721 aagcatttga aaattcttat tccaatagcc tcggtgctgg caatgataat tgtatcatgt
     3781 caaagggaaa ttacgcgtat tgaaccggaa attcctttta atccttttga tacaattgat
     3841 tacagcgatg gtattttaga tgatgtgcca attgattcgg caagtttttt gggattacat
     3901 aaatacatat tcagcaaaac ttgtgcagtg cccggttgtc acgatggtac ttttgaaccg
     3961 gatttcagaa ccgtacaaag tgcttataat accctattgt atgcacctgt tattaaaaat
     4021 gatgctgccg gaacatttac ttatcgcgta attccgggtg atactgcttt atcatggttg
     4081 catgaacgta ttacaactga ggatgaagtg ttaggaagaa tgccgttata cgatacacta
     4141 tatcctgaag aaagagaaaa aattacgcaa tggatattaa atggtgcacc ggatgtattt
     4201 aatcagtccc caacattagc cgattatcaa cctgttactt atggttttat tgcctatgaa
     4261 aatgatacaa atggcatgcg tttagatact attcgtgcag atttcttttc accggttcag
     4321 gttcctgcta attccaatgt tgaattttgg tttggatttt atgatacgga tgaaaatggt
     4381 gtttttcaaa tgccgtcttc attgcaggaa aatattattc gtgtttcaca agatatgttc
     4441 atgaacaccg gttatacaga atatactttt gaactggaac ctaatttcga accattttat
     4501 ggcccaacgt attacgaccc attggttgaa gtaacttatt ggcatcattt caccatcgat
     4561 accgatgatt ttgaaccggg tcaggtgtat tttatgaggg cttatattca ggacaatgca
     4621 catgattttt taactgaaat acctgacgat tcgcaattgt atataatggg gtatttttca
     4681 tttgttgtag aatgattaaa aacacaattt tagctgtcgc tgcaactttg gtaataggtt
     4741 gcggtaagga agatgacata attaatccgc caatgagcga tgtgccagcc attgagttaa
     4801 ttaatgtttc accatctacg gtaactgcat tgggcgattc tatcgttttt attattcact
     4861 acgaagatgg caatggcgat ttagggttca acgcccctga ttcaatgtcg ttatacatta
     4921 ccgataccag gattccgctc acagaatcgt tttttgtccc tctgcttgcc ccggaaggtg
     4981 ctgacatagc cattcagggt gagctggaag tggtgcttaa taatacgatt ttggtagacc
     5041 cggaagccac ttccgaaact gtgcagtttg agatacaact ccgcgacagg tccggcaatt
     5101 acagcaatgt tttggtagcc cctacaataa cagttttgcc tgagtaataa gatttcacag
     5161 ccaataatga attccacatc tttaagtgga aagaaaggtc ttttttgtca ccccttatga
     5221 cattattatg acaattgaca ggctatagtt agggaatttt gcagcgtcat ttttggttac
     5281 tctatttaat acgcgacctt cttgtgaatc aatataaagt tttaacagta acgcataaac
     5341 atgttccggt agagcaatta aagcactaca ttattgattc agacaaatca tcacctgaat
     5401 ttggacataa attacacttc ataaaacaaa atatgcatct cgatgagttg atgtatttaa
     5461 atacatgcaa cagagtgacg tattttttca ccacaaaaaa tcagttaggc tcaggtttta
     5521 ccgctaactt ttttaaaaca attaatcctg catttgaagc ttgtaatgca gttaactcca
     5581 atatgtatga aggggttgaa gcccttgaac atttgtgtga agtaagtgca tcgttagatt
     5641 caatggtggt gggtgaacgg gaaataataa gacagttacg cgtagcttac gatgattcca
     5701 ataacctgaa gctgacagga gacgatattc gcctagcgat gaaaatgctg attcctgcgg
     5761 ccaaaaaaat ctattctgaa acaaaaattg cagaaaaacc catttcagtt gtttcattag
     5821 cagctttaca attaaaagca cttaaacttt cacctgaagc aaaaatccta ttaattggag
     5881 caggtgaaac tattaccaat atgtccactt atttagttga catgggtttt aaaaatttta
     5941 tcgtatttaa caggacctta gcaaatgctg aaaagctggc gcaggctatt aacggcaaag
     6001 catatccgct tgcggagtta gaaaattata cagatggatt tgatgtaatt attacctcaa
     6061 caggttcagc aaaaaaagaa attacagcgc ctgtttatca aaaactggta ggtagtaaca
     6121 aggataaaaa agttattatc gatttagcta ttccattgga tacagatgaa tcgattatta
     6181 ctgattttaa tgttacgtat attaaaattg ataccttaag agcaattgca caaaaaaatc
     6241 tttcatcacg caaagcagaa gtgcttaaag ccaaacacct tgtgcatgaa tttgtaaaag
     6301 aatttcaggc ggtgtatgtg gagcgtatgg tggaaaaagc tcatagtata attccgatta
     6361 aactcaacga aatacgtact aaggctgttg aagaagtgta tgctaaagaa atggcaagct
     6421 tagacgataa cgccaaagag gttgtagata aattattgga ttacatggag aagaagtatc
     6481 tcgcacttac tatggctgct tctaaagatg ccatgcgaca taaacactga taaatacagt
     6541 tcacatgcag gaaacgatta ttattggctc acgcggaagc gacttggcat tgtggcaggc
     6601 tcatttttta caaaaaactt tattggaaca aggttatcgc tcagaaataa aaataataaa
     6661 aacacaaggc gataaaattc aacatatcag tctcgaaaaa ctggaaggca aaggattttt
     6721 caccaaagaa atagaagatg cattattaat gggtgaaatt gatgttgccg ttcactcaca
     6781 taaagattta cccacttcaa ttacacctgg cttaacaata gcagccgttt cgcatagaga
     6841 agatccggct gagttattgc tgattagaaa tgaaaaggta gatgaactca acgaatttaa
     6901 tttaacaaca aatgcgatag taggtacatc atcttcacgc agaaatgttc aaatgcgttt
     6961 ttttcgccct gatttacaat ttgcagatat acgaggaaat gtgcctaccc gaataaataa
     7021 attacgcaat ggcgattacg atgccatttt attggctttt gcaggtgtgt atcgtctgca
     7081 attagatatc agtgatttaa ctgcagttaa aattccatca cataaattaa taccatcacc
     7141 tgcacaaggg gttttagcat tccaatgtcg cgaaaaggat gaacatatga ttggtattct
     7201 tgaaaaaatt aatcaacctg atgttgccga taccatttat attgaacgca aggtgatgaa
     7261 tttatttaat ggtggatgtc acatgccgct tggtgtgtat tgtcgtaaac acaatgaaca
     7321 ataccaggtt tgggctgcac aaacggctga taggttgttg ccgctcaaac gattattcgt
     7381 ggaagggaat aatgcagacc aattggcaac acatattttt aatactttac aacaacagca
     7441 acaaaaatcc atttttattt ctaccgattt gaatgaggat gcgttgtatc tgcaagtatt
     7501 acgcgataaa ggatttgaag taataggaaa atcatgtgta cacatggaat ttattccgtt
     7561 tcaattttct gaggcaactg actgggtatt ttttaccagt aaaaacggag tagattattt
     7621 ttttaattcg gttaaatcca tctctccaaa aattaaattt gctgcaatag gagaggccac
     7681 tgcaaaacag ataaatcaac atggttatgc tgttgagttt gttggagaag gcactatcgc
     7741 acaaattgca gcacagttta aattattagc agcaggtgaa gatgtagtat ttgcagttgc
     7801 ggaaaacaga caaactgcac ttcaatcgca cattgcacag tttgcactaa cgactattat
     7861 tcctgtttat aataatacgc ctattgccat tgaacccgtt caggcagata tttatgtgtt
     7921 tacaagtcct ttaaatgtag acggattttt acttaacaat aacattccat gcaccgctaa
     7981 aatagtagct attggtgcta ctacacagca gcatttaata gataaaggtt atacgcaggt
     8041 ggcagtgccg ccattccaca atttattcag tgttgcagaa ttaatttgcg gctggtaata
     8101 aattattcga ctattatttt cccggtttga ttaccttctt tagtaatcaa tttatacaaa
     8161 taaataccgg gtatccaatt ttcacaattg ataattagtt gttgctgacc ggcaattgat
     8221 tttgtataaa caatttcgcc atttatcgaa gaaatagtaa tattatcgta aacctggctc
     8281 aattgaaaaa ttacacttga attggttggt tgcggataaa tggtaggagc aattagcggt
     8341 aagttactta ctccgttaaa attacatccc ccaatagcat catataatgt ttgtaacgaa
     8401 tcgatagggt caatgctgtc gccttcaaca tcaaacacac gaaggtcaat taaaaaactt
     8461 ccgggctcat cggttggacg cgcaacacgc aaaaatgatg acaacgcaga aatatcaaat
     8521 tctgcacatc ttgtatcagc ccccggcact ttagcacctt gtaatgctga catcagttta
     8581 catcctaaat taccctcagt tgccaaaaaa ttagcttcca tattgtctaa aatttcaggt
     8641 cccaataaaa tatttccctg aatggcataa gtaggtccgg tta
//
LOCUS       DAORKW010000091         8602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_215_length_8602_cov_4.750790, whole genome shotgun sequence.
ACCESSION   DAORKW010000091 DAORKW010000000
VERSION     DAORKW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215_length_8602_cov_4.750790"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..936)
                     /locus_tag="PLB46_05395"
     CDS             complement(79..936)
                     /locus_tag="PLB46_05395"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRF75982.1"
                     /translation="MQSVRRYFTIGFLVSLILGGKVYAQEVEIGAWGGITNSYGDINN
                     TLESLQFVEPGAGVFFRYNVNPRIGYYLGLSGGRTYGYDSISSNPYQLRRNLSFRSDI
                     YDFTGRVDFNFLPLEREKPKNWFTPYVFIGISVFYFNPQAYFDDEWVDLQPLGTEGQQ
                     FPELTGNEKYQRVQVNVPMGGGFKFALNKNITMGIEANWHWLFTDYLDDVSKTYVDPS
                     ILATGADGAQAVALSDRSITFDFLPLGEPGKQRGDSQHNDKYLYTGIFLSYTFVDLKC
                     PGPGKWGTR"
     gene            complement(1328..2956)
                     /locus_tag="PLB46_05400"
     CDS             complement(1328..2956)
                     /locus_tag="PLB46_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75983.1"
                     /translation="MKLLMKIGFFFFVFTTLKLNAQWSNDPANPLLLSTAPDNQNKPV
                     IITDNNEGNFVFWLDKRSGITNVYGQHLDKMGNQLWESNGRLIVESPIDISDINAVLN
                     NEGNILLAWYRYADTLFIQKYDSDATPLWATPTKVAGDDATLTNTFWGPVGPIAMLLN
                     GDGCFLAYNLTGWGYDNLLYNKVEGDGSLPWGYNGIQHVDMSPAGIGKIKLFSDGAGG
                     AFLQSFRGRYYFSRFDSNGNVLWGEVAMDECTGGTGGTYASHTYYEMQTDNDNGWIAA
                     WTSASDDVFATRIDEDGAFVWTPECQPILENPNQQEDIAFTKSGDGYYATWRDSRLDA
                     PGIYMQKINDEGVTQWDEPLYVEDTSLYIPVMKSIATSEGDIVVFYQTSWYFAAQKVH
                     ADGSTAWPQPLKILEGAYNPFYEDYQLFATADNNVIGVAQYSSGLESNIVMFNLNWIS
                     GDTITVDPVAVQTNTIVDFNMSPNPVVQNLQIQSQYPIDNISIIDLNGKTILSQNENN
                     IYSKELYLNQLPSGVYFIKVTSDDNYSVRALVKQ"
     gene            complement(3121..4005)
                     /locus_tag="PLB46_05405"
     CDS             complement(3121..4005)
                     /locus_tag="PLB46_05405"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRF75984.1"
                     /translation="MQVAIYGRLIKKDALPYVQQLFDLLHTRNIKCLIHEEYYPHLVN
                     NIQFKQHPEIFYEKDKDKIKNIDFMFSVGGDGTLLDTITIVKDTKVPIVGINIGRLGF
                     LASIGKDGIEDMIDALEQGTFSLDERSLIHLDSNMPELFGGDNFALNEFTIHKKDSSA
                     MITIHTYINGEFLMSYWADGLIVSTPTGSTGYSLSCGGPIIFPTSENFVITPVAPHNL
                     NIRPIVVSDESVISFEIEGRSEHFLVTLDSRFETVDATVQLAVRKENFTIALIRPNEQ
                     NFLDTLRNKLGLGVDQRN"
     gene            complement(4009..4674)
                     /locus_tag="PLB46_05410"
     CDS             complement(4009..4674)
                     /locus_tag="PLB46_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF75985.1"
                     /translation="MLAKDIISENIPPLKIKDTGAKAIEWMYEFKLSHLPLVDHQNYI
                     GLVSEEDILDFNDTHEQLGKFLKNLYKPFVKDTEHIYEVLRVAANLKTTVIPVVDSEQ
                     HYIGLITLQSLLYNFAKMSSISEPGGVIIIELNNKTDYVLSDIARIVEGNDAHILSLY
                     FNVEPDSGKHAVTIKVDSTEIKHIVATFERYQYQVKAYFQESDMVEIIKDRYDSLMNY
                     LNI"
     gene            complement(4676..5434)
                     /locus_tag="PLB46_05415"
     CDS             complement(4676..5434)
                     /locus_tag="PLB46_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF75986.1"
                     /translation="MNHTIIEENGFKYVEEGEGEVLMLLHGLFGALSNFYDIIEGFKK
                     THKVVIPILPLYDLPIEETTVGGYVNYVERFVAYKGYQKVIVLGNSLGGHIALIYALA
                     NPDKIKGVVLTGSSGLFESAMGDSYPKRGDYDYIKKKAELTFYDPKFASKELVDELFD
                     IVNDRAKVIRVLATAKSAIRQNLAEEVAKISAPTLLIWGNNDTITPPFVGEEFHKLIP
                     NSQLFFIDKCGHAPMMEQPEEFNRYLGKFLNEIN"
     gene            complement(5495..6067)
                     /locus_tag="PLB46_05420"
     CDS             complement(5495..6067)
                     /locus_tag="PLB46_05420"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRF75987.1"
                     /translation="MKILLLDNYDSFTYNLADYLSQNGATPIVKRNDAITLAEIRNLK
                     ISAIVISPGPKRPEDAGITMDLIHHFHATLPILGVCLGYQALGAYFGASVVRSPLPQH
                     GKTSKIDHNGSGLFKNIPSPTEVMRYHSLNIAEIPTILEITAFTQDTNEPMALQHQSL
                     PLAGVQFHPESVLTPHGKQLLANWLDSLPR"
     gene            complement(6064..6615)
                     /locus_tag="PLB46_05425"
     CDS             complement(6064..6615)
                     /locus_tag="PLB46_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRF75988.1"
                     /translation="MIIDIIFAIVLLLAFIHGYKKGIIHSIVSLIALVIGIMAAVRLS
                     ELAAVYLDRWFNIASRYLPLISFILVFIGIYLLFRLLEQMLEGFFKMIKLNFINQLIG
                     AVVWCVIWIMLYSTLLFYINNMNLIADETKKDSITYETIAPFAPKTIEFVGNIIPPVK
                     NVFNTLEIWFDEFELNHTSDNSQ"
     gene            complement(6655..7107)
                     /locus_tag="PLB46_05430"
     CDS             complement(6655..7107)
                     /locus_tag="PLB46_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRF75989.1"
                     /translation="MSIFDQVNEGIKNAMKNKEEATLRALRGVKSALLLAKTEKGAAD
                     ELSEEKEIQVLQKLVKQRKESLEIYTTQNRPELAAVEAEEIAVIEKFLPKQMDEATLR
                     TELQIIITAVGAAGPQDMGKVMGAATKQLAGKADGKMISQLVKELLAK"
     gene            7258..8388
                     /gene="bshA"
                     /locus_tag="PLB46_05435"
     CDS             7258..8388
                     /gene="bshA"
                     /locus_tag="PLB46_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HRF75990.1"
                     /translation="MKIGIVCYPTYGGSGVIATELGKALADKGYKVHFITYKQPFRLD
                     SFHENIFFHEVNFSNYPLFEYPPYETALASKIVDVALFEKLDLLHVHYAIPHASAAYI
                     AKQILKEKNYHLPFITTLHGTDITLVGKDETFIPVVTFSINQSDGITSVSQSLKDETY
                     QNFDITKEIAVIPNFIDFSRFKKLDKEHFRKVIAKNGEKIIVHTSNFRKVKRVEDVVK
                     VFCKIKESIPAKLLLVGDGPERQNVEKLTRQVCMEDDVYFLGKQEAVEELLAIADLFV
                     LPSETESFGLAALEAMACEVPVISTNTGGIPEINVQGVTGYLSNVGDVDDMAANAIKI
                     LSDESTLLQFRKNAIKQANKFSLDNILPLYEAYYKKVVNVQD"
BASE COUNT         2675 a         1609 c         1646 g         2672 t
ORIGIN      
        1 gcaatgtgtt gaggtatatt atttaagtcg agttgaacgg cggacatcag ggtgcaaatt
       61 tatcaaaaaa ggatggtgtt atcgtgttcc ccatttgccg ggtccggggc attttaagtc
      121 aacaaatgta taggatagga aaattccggt gtagagatac ttatcgttat gctgactatc
      181 gccgcgttgt tttccgggtt cacctagtgg aagaaagtcg aaggtaatac ttctatcgct
      241 caaagccaca gcctgtgcac catcagcacc ggttgccaat atcgaagggt ctacgtaggt
      301 tttactcaca tcgtcgaggt aatcggtaaa caaccaatgc cagttagctt caatacccat
      361 ggtaatattt ttgttgaggg cgaatttaaa tcctccaccc ataggtacat ttacctgaac
      421 tcgttggtat ttttcattgc ctgttagttc gggaaactgt tgcccttctg ttccaagtgg
      481 ctgtaaatca acccattcgt catcaaaata ggcctgcgga ttaaaataaa acacagaaat
      541 accaataaaa acataaggtg taaaccaatt tttaggtttt tcgcgctcca ggggcaaaaa
      601 gttaaagtct accctgcctg taaaatcata aatatctgac ctaaaactga gatttcttct
      661 tagttgataa gggttagaag agatagaatc gtagccataa gtgcgtcctc cgctcaagcc
      721 aaggtaatat ccaatacgcg gatttacatt gtatcgaaaa aaaacacctg cacccggctc
      781 tacaaactga aggctttcga gggtattgtt aatgtcgccg taagagttgg taatgccgcc
      841 ccaggcgccg atttcaacct cctgggcata cacttttccc cctaagataa ggctcactaa
      901 aaaaccaatt gtaaaatatc gacgaacaga ctgcattaaa aggattgcaa agttacggaa
      961 actaggggtt aacgcataga gattgagatt attgtactta aacggttaat tgagcggtag
     1021 caggtggttt ttgtagtgga tagatgcaat tctagcttcg ggttttactg ccagttgaat
     1081 agcttaatta taaaaagatt gcagtgggtt gggtgctatg cctacgcagt tatccattat
     1141 ttgtagggcg gatgcaattc gcccctacaa ataatggata actgctccat ttgagttaaa
     1201 cttcacagtt ttaggaaaaa tttcaatcaa gagaacaagg gtgatttact caaaccgact
     1261 gcctatcgcc gactgctaac tgcatatcac tctcaaaatg ttacttgtag accaaaactc
     1321 aaaaaagtca ttgtttaacc aaagctctta ctgaataatt atcgtctgat gtaactttta
     1381 tgaaatatac tcccgatggt aattgattta aatagagttc ttttgagtaa atattatttt
     1441 cgttttgcga taatattgtt tttccattta agtcgatgat tgaaatatta tcgataggat
     1501 attgagattg aatttgcaga ttttgcacca caggatttgg gctcatatta aaatcaacaa
     1561 ttgtatttgt ttgaacagca accggatcaa ctgtaatagt atcaccgcta atccaattta
     1621 aattaaacat cacaatattg gattcaagtc cggaagaata ttgtgcaacg ccaattacat
     1681 tattatcggc tgtagcgaat agctgatagt cttcataaaa aggattgtat gcaccttcta
     1741 aaattttgag cggttgcggc catgcagttg aaccatcggc atgtactttt tgtgctgcaa
     1801 aataccagct tgtttgataa aataccacga tatctccttc tgatgttgca attgatttca
     1861 taacagggat atataaactt gtgtcttcaa catataaagg ctcgtcccat tgcgttactc
     1921 cttcatcatt aattttttgc atataaattc caggcgcatc gaggcgacta tctcgccagg
     1981 ttgcataata tccatcgcca ctttttgtaa atgcaatatc ttcctgttga tttgggtttt
     2041 ctaaaattgg ttgacattcc ggggtccaaa caaaagctcc gtcctcgtca attctagttg
     2101 caaatacatc gtctgaagca cttgtccagg ctgcaatcca gccattatca ttatcggttt
     2161 gcatttcgta atacgtatgg ctggcataag taccgccggt gccacctgtg cattcatcca
     2221 tggcaacttc tccccaaagt acattgccat tactatcgaa tcgtgaaaaa taatatcgac
     2281 ctctgaatga ttgtaaaaat gcgccgcctg caccatcgga aaataatttt attttaccaa
     2341 ttccggcagg gctcatgtct acatgttgaa tgccattata tccccaaggt aatgaaccgt
     2401 cgccttcaac tttattgtat aataaattat cgtaacccca accggtaaga ttgtaggcta
     2461 aaaaacaacc atctccattt agcaacatgg caattggtcc aacaggtccc caaaaagtat
     2521 ttgttaatgt agcatcatca cctgcaactt ttgtcggagt tgcccataat ggtgttgcat
     2581 cggaatcata tttttggata aacaatgtat cagcatatcg ataccatgcg agcaaaatat
     2641 tgccttcatt attcaatacc gcattgatgt cagaaatatc aattggactt tcaacaatta
     2701 atcttccatt tgattcccat aattgattgc ccattttatc aagatgttgt ccatatacgt
     2761 ttgtaattcc tgaacgttta tccaaccaaa aaacgaaatt accttcattg ttatcggtaa
     2821 tgattaccgg tttattttgg ttgtcgggcg cagtgctcag cagcaaggga tttgcagggt
     2881 cgttgctcca ttgtgcgttt agttttaacg tggtaaaaac aaaaaagaaa aatccaattt
     2941 tcattaacag tttcataatc ggtagtattt aaacacaaag gtaggcgtgg ggtagaggcc
     3001 aaagtagtag aaaaatggcg gattgtagca acacctataa actgcctcct atccctgatt
     3061 gagctaggca ggtagtgaat aatatataaa atcgtaaatg cagcagtaag cgcaggctga
     3121 ttaattccgt tgatcgacac ctaaaccaag tttattccgc agcgtatcga ggaaattttg
     3181 ctcgttaggg cgaattaatg ctatggtgaa attttctttg cgcaccgcaa gttgaacggt
     3241 ggcatctaca gtttcgaagc gactgtcgag ggtaactaaa aaatgttcgc ttcttccttc
     3301 aatttcaaaa cttataaccg attcgtcaga aacaacaatt ggccgaatat ttagattatg
     3361 tggtgcaacg ggagtgatga caaaattttc ggaggttgga aaaataattg ggcctccgca
     3421 acttagtgaa taaccagttg aacctgttgg tgtagaaaca attaatccat cggcccaata
     3481 actcattaaa aattcaccat taatataagt atgaatggta atcatggcag atgagtcttt
     3541 tttgtgaatg gtaaattcat tcaaagcaaa attatctccg ccaaataatt caggcatatt
     3601 ggaatcgagg tgaattaatg aacgttcatc taaactaaat gttccttgtt caagcgcatc
     3661 aatcatatct tcaattccat ctttaccaat actcgcaaga aatcctaatc ttccgatatt
     3721 aattcctaca attggcactt tggtatcttt cacaattgtg atggtatcga gcagagtacc
     3781 atcaccacca acactaaaca taaaatcgat attcttgatt ttatctttat ctttttcgta
     3841 aaatatttcc ggatgttgtt tgaactgaat attatttacc aggtgcggat aatattcttc
     3901 atgaattaaa catttaatat tacgggtatg taataaatcg aaaagttgct gcacataagg
     3961 cagtgcatct ttttttatta atcttccgta aatggcaacc tgcatatatt aaatattgag
     4021 gtaattcatt agtgaatcgt agcggtcttt gatgatttct accatatcag attcctgaaa
     4081 atatgctttt acctgatatt ggtaacgttc gaaagtagca acaatatgtt ttatttcggt
     4141 gctatcaact ttaatagtaa cagcatgttt tccggagtcc ggttcaacat taaaatacaa
     4201 actgagaata tgcgcatcgt taccttctac aatacgtgca atatcactta acacataatc
     4261 agttttgttg ttgagttcga taataattac accacccggt tcgctgattg aactcatttt
     4321 tgcgaagttg tataataaac tttgcagtgt aatcaatccg atataatgtt gctcgctatc
     4381 tacaaccgga attacggtag ttttcagatt cgctgcaacg cggagcactt cgtaaatatg
     4441 ttcagtgtct ttgacaaatg gtttatacaa attttttaaa aatttgccca gttgttcatg
     4501 tgtatcatta aaatcaagaa tatcttcctc gctaaccagt ccgatataat tctggtgatc
     4561 aaccaatggg aggtgagaga gtttaaactc atacatccac tcaatagctt ttgctccggt
     4621 atcttttatt tttaacgggg gaatgttttc cgatatgatg tcttttgcca gcacgttaat
     4681 taatttcgtt taggaactta cccagataac gattaaactc ttccggttgt tccatcattg
     4741 gtgcatgtcc gcatttatct ataaaaaata actgcgaatt tggaatgagt ttatgaaact
     4801 cttcaccaac aaacggtggg gttatagtat cgttattacc ccatatcagc aaggttggtg
     4861 ccgaaatttt tgccacttcc tctgccaagt tttggcggat agcgcttttt gcagttgcca
     4921 gcacacgaat aacttttgct cggtcgttca cgatgtcaaa aagttcatca accaattctt
     4981 tagaagcaaa ttttgggtcg taaaacgtga gctccgcttt tttcttgata taatcgtaat
     5041 cgccgcgttt cgggtaactg tcgcccatgg cactttcaaa caacccggag ctaccggtta
     5101 aaacaacacc tttaatttta tcgggattag ccaaagcata aatgagtgcg atatggccgc
     5161 ctagtgaatt acctagtaca atcacttttt ggtatccttt gtaggctaca aaacgctcca
     5221 cataatttac ataaccgcct acagtagttt cttctatagg taaatcgtaa agtggaagta
     5281 tcgggataac aactttatgt gttttcttaa atccttcaat tatgtcataa aagttgctca
     5341 gggccccaaa taaaccgtgc aacagcatca gcacttcgcc ctcgccctct tctacgtatt
     5401 taaatccgtt ttcttcaatt atcgtgtgat tcatacctgt cagtaaatca caaaaatatg
     5461 aattttgaat cgttattata gatttaattg gtagttagcg gggtaaactg tccaaccaat
     5521 tggccaaaag ctgtttccca tgcggtgtca gcaccgattc ggggtgaaac tgaacaccag
     5581 ccaaagggag cgattggtgt tgtaatgcca tgggttcgtt ggtgtcttga gtgaaggcag
     5641 ttatttccaa aatagtaggg atttccgcaa tattcaggct atgatagcgc ataacctctg
     5701 ttggcgaagg aatattttta aacaaacctg agccattgtg gtcaatttta gaggttttgc
     5761 cgtgttgggg aagcggagaa cggacaaccg atgccccaaa ataagcgccc agagcctggt
     5821 agccgagaca tacccctaaa atgggcaaag tggcgtggaa atgatgaata aggtccatgg
     5881 taattccggc atcttcaggg cgttttgggc ccggcgaaat aacaatagcg gaaattttca
     5941 gattccggat ttcagcaagg gtgatggcat cattgcgttt tacaataggt gttgcaccgt
     6001 tttgggataa ataatctgca agattataag taaatgaatc gtaattatcg agcagtagaa
     6061 ttttcattgc gaattgtcgg atgtatgatt gagttcaaac tcatcgaacc agatttcgag
     6121 ggtattaaat acatttttta ccggtggaat aatgttgccg acaaactcaa tagtttttgg
     6181 tgcaaaagga gcaattgttt cgtaggtaat ggaatctttt tttgtttcat cggcaatgag
     6241 attcatattg ttgatataaa acaacaatgt tgagtataac ataatccaaa taacacacca
     6301 tacaactgca ccaataagct gattgataaa attgagtttt atcattttaa aaaagccttc
     6361 aagcatttgt tctaataacc taaataataa atagatacca ataaatacaa ggataaaaga
     6421 aattaagggt aaatagcgcg aagcgatgtt aaaccacctg tcaagataca ccgctgcgag
     6481 ctcgcttaaa cgcactgctg ccataatacc aattacgaga gcaattaatg aaacaatgga
     6541 atgaataatt ccttttttat acccatgtat aaatgccagt aataaaacta tagcgaaaat
     6601 aatatcaata atcatgagtg gtaattaggt ggtatgctaa ttaaaagtcg cttattattt
     6661 agccaataat tcttttacca attgcgaaat cattttgcca tcagcttttc ctgcaagttg
     6721 tttggttgcg gcacccataa ctttacccat atcctgagga cctgcagcac caacagctgt
     6781 tataataatt tgaagttcgg ttctcaaggt tgcttcatcc atttgttttg gaagaaattt
     6841 ttcaataacc gcaatttctt ctgcttcaac tgcagccagt tcggggcgat tttgtgtagt
     6901 ataaatttct aagctttctt ttctttgttt caccaatttt tggagcacct gaatttcctt
     6961 ttcttcactt aattcgtcgg cagcaccttt ttcagttttg gctaataaca aggcagactt
     7021 tacaccgcgc aatgcacgta aagtagcttc ttctttattc ttcatggcat ttttaatgcc
     7081 ttcgtttacc tgatcaaata ttgacatatg cgatagttta tattggcaaa tttcgtgatt
     7141 taaaatgagt tattgaaata gggtaagtca tttcaaacag ggcaacatta acattttttt
     7201 tgttttaccg aacaaaacgc aactaaaatg tattaaatat gtcactaact tcacagcatg
     7261 aaaatcggaa tagtatgtta ccccacctat ggcggaagcg gggttatagc gacagaactg
     7321 gggaaagccc tggcggacaa agggtataaa gtacatttta ttacctataa gcaacccttc
     7381 aggttggact catttcatga gaacattttt tttcatgaag taaattttag caattacccc
     7441 ttatttgaat atccacctta tgaaacagca cttgcgagta aaattgtaga tgttgcactt
     7501 ttcgaaaaac tggatttact acatgtacat tatgctattc cgcatgccag tgctgcttat
     7561 atcgccaaac aaattttaaa agaaaaaaat tatcatctcc cttttataac aactttacac
     7621 ggaacagata ttacattggt tggaaaagat gaaacgttta ttccggtggt aactttttcg
     7681 attaatcaaa gcgatggtat tacatctgtt tcgcaaagtt taaaagatga aacgtatcag
     7741 aattttgata ttactaaaga aattgccgtt attcccaact ttatcgactt cagtcgtttc
     7801 aaaaagctgg acaaagaaca ttttagaaaa gtaattgcta aaaatggaga aaaaataatt
     7861 gtgcatactt ctaatttccg aaaagtgaaa cgggtagagg atgtggtaaa agtattttgt
     7921 aaaataaaag aatctatccc ggcaaaatta ttattagtag gtgacggacc agaacgacaa
     7981 aatgtggaga aattaacccg tcaggtttgt atggaagatg atgtttattt tttaggcaaa
     8041 caggaagcgg tagaagaatt attagctatt gccgacttat ttgtattacc atcagaaaca
     8101 gaaagttttg gcttagcggc gttagaagcc atggcatgtg aagtgccggt tatttcgacc
     8161 aacacgggtg gtattcctga aattaatgta caaggtgtaa caggttattt aagtaatgtt
     8221 ggtgatgttg atgatatggc tgccaatgca ataaaaatat taagtgatga atctacttta
     8281 ttgcaattca ggaaaaatgc gattaaacaa gcaaataaat tttcgctgga taatattctt
     8341 ccattgtatg aggcttatta taaaaaggtt gtaaatgtac aagattaatg tgtccgccac
     8401 ggtggatgct aattaaacag ccggagaatg taccgatgta ataatgtacc gatgtaccaa
     8461 tgaaacagcc ggggaatgta ccaatgaaat aatgtaccga tgtaataatg taccgatgta
     8521 ccaatgaaac agccggggaa tggaccgatg taataatgta ccgatgtacc aatgaaacag
     8581 ccggggaatg tacccatgta at
//
LOCUS       DAORKW010000092         8602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_216_length_8602_cov_4.684685, whole genome shotgun sequence.
ACCESSION   DAORKW010000092 DAORKW010000000
VERSION     DAORKW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216_length_8602_cov_4.684685"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..2190)
                     /locus_tag="PLB46_05440"
     CDS             complement(316..2190)
                     /locus_tag="PLB46_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRF75991.1"
                     /translation="MRNLFTLFFICTSFILMAENEVAVTSKINSVTVYTQGAQIYRDA
                     KAEIPVGKTMLVFKDISTNVDKESIQVKGEGAFTIMSVAYKFDYLTIQSVADEIVALQ
                     KKQTEINSQITYENSILNVFKAEQTLIERNQSIGGANTGVTAENLKAMADFQRARLTE
                     IFTKQIEIANKISTLNTEVQKISAQLKELNINITQAKGEIHVLVSSKTALTGKFTLNY
                     YVQNAGWYANYDLRVDKINQPIEINYKANVFQNTGEDWKNVKLSLNNGNPNESGVMPK
                     IYPWRLHYGAITGGIYQPSSYINKTFFSVQGRVTDATGEPLIGATVMVKGGTLGTITD
                     INGNYSLQLSNGSNYLLINYLGYNTVELPITNSVLNVVMQENVQYLEEVVISSKSSKA
                     IGNVGFFEGRTNSEKAEMKTLALETNFQYKATTFTYEIEEPYTVISDGKISTIDIQAV
                     KVKADYEYFAVPKIDRDAFLTANVTDWRELNLLTGEANIFFEGAFLGKTVLDPTYASD
                     TISISLGRDKSVQIQRNKMKEFTQRQFLGGNKIETVAFEIKIRNNKSEPIHIVIQDQF
                     PISSENDVTVEGSIKPAGKIEDATQIITWDMDIVPGEEKKMELKYTVKYPKDKVIVLE
                     "
     gene            2466..2933
                     /locus_tag="PLB46_05445"
     CDS             2466..2933
                     /locus_tag="PLB46_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRF75992.1"
                     /translation="MSTMQLDDLDYKILEKLIKDGRKSFTDIADELNVSVGTIRNRFN
                     VFVENELLTIIGRVNPDKIGFHTYAQILIKVRPVDKVTQVAENIASLPEVSFLAMTTG
                     AYDLEVNVMCRDNEHLVQLMTNHITKMDGIFESATNIYFKVYKYAQPDLYLLK"
     gene            2982..6095
                     /locus_tag="PLB46_05450"
     CDS             2982..6095
                     /locus_tag="PLB46_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF75993.1"
                     /translation="MVKKNSLPLLPLLLIIAIIFMSTASFAQTTITGVVLDSITNETL
                     IGANVRLQSDPTIGASTDIDGKFTISSPTTSGAIIISYIGFNTKTITFNGNSSLNIVL
                     SSEKFLEEVIVVGYGTQKKSDITGSISSTRNEEFRDQPISNIAGSIQGKVSGIDIRNA
                     SGTPGAGLLVSVRGQSNPLYVVDGIPMISESNSGISTSFDLQGASVGSGQNISSISDL
                     NPDDIESIEILKDASAASIYGARAANGVILITTKRGAAGKTKFNLNYYSGIQQLSRPI
                     EFLSSEELVALIEEARLNDLNAYNADPTIFGEDYDPALSTNPLPDSWNTGVNTDWMDE
                     VFRLAPINNVEFSASGGNDKTTFYISNSYFDQQGIVIESFYKRFNSRLNLDHKISDKL
                     KIGENISLTYSNNRRSLNDNVYTGTVTNAIGASPLMPVYDEDGEFANFEDYQASWLSD
                     NPVLSTEEIIPFTKSNRALASIFGEYQIAPGLKFRSTWSVDFTYLTDDLYFSPITTDA
                     EGVGGKATNSTYSQRVWLGENILSYDKYFTNDHAINIIGGFTLQESSSDYLSISGQGF
                     PIGSGLQDVSSAAIITGGDSYASNWALVSFLSRLNYNIKSRYLFSLSARLDGSSRFSP
                     NNRYGFFPAGSAGWHLSDESFWPAALFITDFKLRTSYGITGDQEIGDFQYISFWSPVG
                     YNGQSGLGPRNIADEDLKWQSNKMFNIGFDYELWRGRVSGSFEYYIGNKTDLLSEDVV
                     PGTTGFGSVTRNFGNIQNSGFEFSANTIIVDKSDFDWSFGFNFSYLHNEIIELSTDSI
                     LLSAYTDLTATHILVEGQSYGSFWGVPYEGVDPQTGDPVYTDTNEDGVIDDGDAVILG
                     KATPDWYGGFNTAVKYKNWDAGLAATFSLGNEVYNLIRSTYQTGGWSDYGWDEEFYLY
                     QVYANNSHVIDDRWQQPGDETDIPRASLFNYNFYQNSGQAIEDGSHLRIREISVGYTI
                     KPTSITRFESLRIYAQVQNAYVFSKYTGFDPEVSSTGGDHPETAGVDYAAYPQARTYM
                     LGFNFKF"
     gene            6123..7556
                     /locus_tag="PLB46_05455"
     CDS             6123..7556
                     /locus_tag="PLB46_05455"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF75994.1"
                     /translation="MKFQHKSFNNNIQLNNWMLGSLVVIMTLSFTSCESFLSQTSTND
                     VADGDMFSDAASLESARIGMYNSLQNQYYYGGFFPLMADAYSDNGATGGYDNLDLDEL
                     GYKAATPNNIYIENCWIAIYNTIYNCNQIITNIDDINDPLLSEELRNDIKGEALTVRA
                     LAHFDLLRMFGEHWDNTSTLGIPVVKEILSPETTIGRNTVAETYDAIINDLTTAEGLL
                     INDDLRFDPPALKGPQFITKTACQALLARVHQYHFDYINADYYASLVILEGFAGLSDA
                     NSYAETYATKFATEAIFELAFNGQDKSQFNALTFARPDALRSEVSFLAAEDLSAFFST
                     RADDVRAGLVDFENNDVSIEPDGRTQKYRGEVFQDNSAYVIRYAEMLLILGETGGFAG
                     GPIVLNDLRAARGLSNATPADEAEYVALLLDERRAELNFEGHRYFDLAHFGLVESVLG
                     EEVMPCFPIPGREINASGGLLIQYPGY"
     gene            7589..>8602
                     /locus_tag="PLB46_05460"
     CDS             7589..>8602
                     /locus_tag="PLB46_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF75995.1"
                     /translation="MNAFKSINPFNQELIAEHPVFTSEQIDAALTNAEKAFHNWKTTS
                     FEQRSDLLFKLSTVLKNNKEQYALHIALEMGKILAEARAEVEKCALACEYYAENGPIL
                     LQDRIIESDARKSLVAYDPIGAVFAVMPWNFPFWQVIRFAAPTIMAGNVALLKHAKNV
                     SLCAKDIEAAFIEAGFPTGVFQSLIAQANQSEQIIAHDIVQGVTLTGSENAGAAVALL
                     AGKHIKKSVLELGGSDPFIVLDDADVHAAAKIATKSRMQNAGQSCIAAKRFIVTQKIE
                     QDFIQAFKNEIALIHQGDQLLATSTMGPVSSVVAADELTTQQQQSVDLGAQIITGGFK
                     NGAN"
BASE COUNT         2713 a         1493 c         1627 g         2769 t
ORIGIN      
        1 aattagttgt tacataaaat aaaaaaagcg gcttttttac agaaagccgc tttttttatt
       61 tgtataattt gtaccggtcg ggagaccggg gtggcgtctg gagtcggaga ctccacgaca
      121 actcgaagcc atgaaatttt cggtaaacat tgcatgttta gtaatgattt aaatttggct
      181 acaacaaccc cacatatacc acacagcaaa aatgtagtca tagactacat ttttgctgtt
      241 gtcgtggagt ctttgactcc agacgccacc ccggtctcca gaccgaaagg caacattcaa
      301 tgttgctgta tttaattatt ccagcacaat tactttatcc ttcggatact taaccgtata
      361 ttttaattcc atttttttct cctctcccgg aacaatatcc atatcccaag ttattatttg
      421 cgttgcatct tcaattttac ctgcaggttt aattgaacct tctacggtta catcattttc
      481 tgaggaaatt ggaaattggt cttgtattac aatatgaata ggttctgatt tattattgcg
      541 gatttttatt tcaaatgcaa cagtttcaat tttgttgcca cctaaaaact gacgttgggt
      601 aaattctttc attttgttgc gttgaatttg tacactttta tctctaccca aagaaataga
      661 aatggtatct gatgcatacg tcggatctaa taccgttttg ccgagaaacg ctccttcaaa
      721 aaagatgttt gcttcaccgg ttaataaatt taattcacgc caatctgtta cattggcagt
      781 taagaatgca tcgcgatcaa ttttagggac tgcaaaatat tcataatcag ctttcacttt
      841 tactgcctga atatctattg tggaaatttt accatcactt attacagtat agggttcttc
      901 aatttcatac gtaaatgttg tcgctttata ttgaaaattg gtttctaaag ccagggtttt
      961 catttcagct ttttcactat ttgtcctacc ttcaaaaaat ccaacattac cgatagcctt
     1021 gctacttttg ctggatatta ccacttcttc taaatactga acattttcct gcataaccac
     1081 gtttaatacg gagttagtaa taggaagttc aaccgtattg taaccgagat aattaattaa
     1141 caaataattg gagccattgg aaagttgtaa tgaataattg ccattaatat ctgtaatagt
     1201 tcccaatgtt cctcctttta ccattaccgt tgcacctatt aaaggttcac cagttgcatc
     1261 tgtaactctt ccctgcactg aaaaaaatgt tttattaata taactactag gctgataaat
     1321 accacctgtt atggcaccat agtgtaatcg ccatggataa attttaggca ttacaccact
     1381 ttcattcgga ttgccattgt tcagcgacaa ttttacattt ttccaatcct caccggtatt
     1441 ttgaaataca ttcgctttgt aattaatttc aataggttga ttgattttat ctacccttaa
     1501 atcataattc gcataccatc cggcattttg cacataatag tttaaggtaa atttaccggt
     1561 taacgccgtt tttgatgaaa ccaaaacatg aatttcgcct ttcgcctgcg tgatattaat
     1621 attcaattct tttagctgag cagaaatttt ttgcacttca gtatttaatg ttgaaatttt
     1681 attggctatc tcaatttgtt tggtaaaaat ttcagttaag cgtgctcttt ggaaatcggc
     1741 catggctttt aaattttcag ccgttacacc agtgttggct cctccaattg actggttacg
     1801 ctcaattaac gtttgttctg ctttaaaaac gttgagtata gaattctcat aggtgatctg
     1861 actattgatt tcagtttgtt ttttctgtaa agcaacaatt tcatcggcca ccgattgaat
     1921 agtcaaataa tcgaatttat aagcaaccga catgatggta aaagcacctt cgccttttac
     1981 ctggatactt tctttatcta cattcgtaga aatatcttta aacaccaaca tggttttgcc
     2041 taccggaatt tccgctttgg catcgcggta aatttgggcg ccctgagtgt aaacagttac
     2101 actgttaatt tttgaagtaa cagcaacttc attttccgcc attaaaataa aactagtgca
     2161 aataaaaaat agtgtgaata gattacgcat ggttataaat ttatagttca aaataacgtc
     2221 aaataagcta tactattata taattgaaca tacaacgctc aaattatggc tgaattttta
     2281 cctcaagctt aaaaatgtag agtaagtttt ttggtatagc tagtgagaaa attaacccta
     2341 aaacttgatg gtaacttgca tttggcataa aaaaaatgca aaaatcggct aatatttgtc
     2401 acttttattg aaaaaaactt tgaaatattg taaattattc cattatttgt ataaatctta
     2461 gcctgatgag caccatgcaa cttgacgacc tggactataa aatccttgaa aagttaatca
     2521 aggatggacg taaaagcttt acggatatag ccgatgaatt aaatgtatca gttggaacta
     2581 tacgcaaccg gtttaatgta tttgttgaaa atgaactgct cactataatt ggcagggtta
     2641 atcccgacaa aattgggttt catacctacg cacaaatctt aattaaggta aggcctgtag
     2701 acaaagtaac acaagtagca gaaaatatcg cgagtttgcc tgaagtgagt tttttagcaa
     2761 tgactaccgg tgcttacgac ctggaagtaa atgttatgtg ccgcgataat gaacacctag
     2821 tacaattaat gaccaatcac attaccaaaa tggatggcat ttttgaaagt gcgactaata
     2881 tttattttaa ggtttacaaa tatgcacaac ccgaccttta tctcctgaaa taacacattt
     2941 aaattaccaa ttgattaaac cattaaccaa aacccactac catggttaaa aaaaattcat
     3001 tgccattatt gccattattg ctgattatcg caataatatt tatgagtacc gcctcctttg
     3061 cacaaacaac aattaccggt gttgtactcg attcaattac taatgagacc ttaataggtg
     3121 cgaatgtcag attgcaatct gacccaacaa ttggtgcaag taccgatatt gatgggaaat
     3181 ttaccatttc atcgccaacc acttcaggtg ctattattat ttcttacatt ggatttaata
     3241 caaaaacaat aacgtttaat ggcaacagtt cattaaatat tgtattgagt tctgaaaagt
     3301 ttttggaaga agtaattgtg gttggttatg gcacacagaa aaaaagtgat ataacaggct
     3361 ccatatcttc aacacgcaat gaagaattcc gcgaccagcc aatttccaat attgccggaa
     3421 gtatacaggg aaaagtttca ggaattgata tccgcaatgc ctccggaaca ccgggtgcag
     3481 gattgctggt gagtgttcgc ggtcaaagta atccgcttta tgtagtagat ggaattccta
     3541 tgataagcga aagtaattcg ggaatttcca cctcattcga tttacaaggc gcttcagtag
     3601 gttcgggaca aaatatttcg tctatttctg atttaaatcc ggatgatatc gaatccatag
     3661 aaatattaaa agatgcatct gctgcttcta tttatggtgc acgcgctgct aacggcgtaa
     3721 tattaatcac tacaaaaaga ggcgctgccg gaaaaacaaa atttaattta aattattatt
     3781 ccggtatcca acagctgagt cgacctattg aatttttaag ctctgaagaa ttagttgcct
     3841 tgattgagga agcaagatta aatgatttaa atgcttataa cgcagaccct accatttttg
     3901 gagaagatta tgacccggca ctttcaacaa atcctttacc ggatagctgg aacacaggtg
     3961 taaataccga ttggatggat gaagtttttc gtttagcacc aattaataac gtagaatttt
     4021 ctgcttcagg tggtaacgat aaaaccacat tttatatttc taattcctac tttgatcaac
     4081 aaggtatagt tatcgaatca ttttataaaa gatttaattc gcgattaaat ctcgaccaca
     4141 aaataagtga caaattaaaa attggtgaaa acatatcact tacctattct aacaatcgca
     4201 gaagtttgaa tgataacgtg tatacaggaa ctgtaacaaa tgcaattggt gcctcaccac
     4261 ttatgccggt ttatgatgag gatggtgagt tcgcaaattt tgaggattat caagcgagct
     4321 ggttaagcga taatcctgta ctttcaactg aagaaataat tccatttaca aaaagtaatc
     4381 gcgcactggc ttccattttt ggagaatatc aaattgcacc gggtttaaaa ttccgttcaa
     4441 catggtcggt tgattttacc tatttaaccg atgatttata tttttcacct ataactaccg
     4501 atgcagaagg tgttggcggt aaagcaacaa atagtactta tagtcagcgc gtttggttag
     4561 gcgaaaatat tttatcgtat gataaatatt ttacaaacga ccacgcaata aatataattg
     4621 gcggatttac cttacaagaa tcttcttctg actatttatc tatttcaggt cagggatttc
     4681 caattggaag cggattgcag gatgtatcaa gtgctgcaat aattaccggt ggcgattctt
     4741 atgccagtaa ttgggccttg gtgtcttttt taagccgatt aaattataat attaaaagtc
     4801 gttacctatt ttcattaagt gcacgcttgg atggttcttc tcgtttttct ccaaataaca
     4861 gatatggatt ttttcctgca ggttccgcag gttggcattt gagtgatgaa agtttttggc
     4921 ctgcagcact atttattacc gattttaaac ttagaaccag ttatggtatt acaggagacc
     4981 aggaaattgg tgattttcaa tatatcagct tctggagccc tgttggatat aacggacaat
     5041 cgggattagg accacgtaat atcgctgatg aagatttaaa atggcaatcg aataaaatgt
     5101 ttaacattgg atttgactat gaattatggc ggggacgtgt aagtggttca ttcgaatatt
     5161 atatcggaaa taaaacagat ttattaagtg aagatgttgt tccgggaact accggttttg
     5221 gttcagttac ccgaaatttt ggtaatattc aaaacagtgg tttcgaattt tctgcgaata
     5281 ccattattgt agataagagt gatttcgatt ggtccttcgg atttaatttc agctatttac
     5341 acaatgagat aatcgaattg agtacagata gtattttatt aagtgcttat accgacctca
     5401 ccgcaacgca tattttagtg gaaggccaat cgtatggttc attttggggt gttccttacg
     5461 aaggtgtaga cccgcaaaca ggtgaccctg tttataccga taccaatgag gatggtgtta
     5521 tagatgatgg tgatgccgtt attttaggaa aagcaactcc tgattggtac ggtggattta
     5581 atactgctgt aaaatataaa aattgggacg caggtttagc cgcaacattt agtttaggaa
     5641 atgaagtgta taatttaatt cgctctacct accaaactgg cggatggagc gattatggat
     5701 gggatgagga attttattta tatcaggttt atgccaataa ttcacatgta attgatgaca
     5761 gatggcagca accgggtgat gaaacggata ttcctcgtgc gtcattattt aattacaatt
     5821 tttaccaaaa ttccggacaa gcaattgagg atggttcgca tttgcgtata agagaaataa
     5881 gtgttggtta tacgattaaa ccaacatcta ttacacgatt cgaatcgttg cgtatatatg
     5941 ctcaagttca aaatgcttat gtgtttagca aatatacagg cttcgaccct gaggtgagtt
     6001 caacaggtgg cgatcatccg gaaacagcag gagtggatta tgctgcttat cctcaagcaa
     6061 ggacttatat gctcggattt aatttcaaat tttaattctg tttaccactt taaatgatga
     6121 ttatgaaatt tcaacataaa tcttttaata acaacatcca attaaacaat tggatgttag
     6181 gtagtttagt cgtaataatg acattgagtt ttacctcttg cgaaagtttt ttatcgcaaa
     6241 cttctaccaa cgatgtagct gatggagata tgtttagcga tgctgcaagt ttggaaagtg
     6301 cgcgcattgg tatgtataat agtttgcaga accaatatta ttatggcgga tttttcccgc
     6361 tgatggctga tgcttatagc gataatggag caacaggtgg ttacgataat ctcgacctcg
     6421 atgaattggg atataaagcg gcaactccaa ataatattta tattgaaaat tgttggattg
     6481 ccatctataa tactatttat aattgtaatc aaattattac caatattgat gatatcaacg
     6541 accctttgtt atctgaagaa ttgcgcaacg atattaaagg tgaagcatta accgtacgcg
     6601 cattagcaca tttcgattta ctgagaatgt ttggtgaaca ctgggacaat acttcaacac
     6661 ttggtatacc tgtagtgaaa gaaattttat cacccgaaac tactatcgga agaaatactg
     6721 ttgctgaaac atacgatgcc atcataaatg acctcacaac tgcagaaggt ttattgatta
     6781 atgacgattt acgatttgac ccacctgcat taaaagggcc gcaatttatt accaaaactg
     6841 cttgtcaggc attattagct agagtacatc aatatcattt tgattatatc aatgccgatt
     6901 attatgcttc acttgtaatt ctcgaagggt ttgccgggtt atctgatgca aattcatatg
     6961 ctgagactta tgcaacaaaa ttcgcgacag aagcaatttt tgaattggca tttaatggtc
     7021 aggataaaag tcagtttaac gccctcactt ttgcaagacc ggatgcattg cgcagtgaag
     7081 tttctttttt agctgccgaa gatttatccg catttttctc aacaagagcc gatgatgttc
     7141 gtgcaggttt agtcgatttt gaaaataatg acgtaagtat agaacctgat ggcagaacac
     7201 aaaaatacag aggagaagta tttcaggata acagtgcgta tgtaattcgt tatgctgaaa
     7261 tgttattgat acttggtgaa accggtggat ttgcaggcgg tccaattgtg ttaaacgatt
     7321 tgagagcagc aagaggtttg tctaatgcta cacctgcgga tgaagcagaa tatgttgctt
     7381 tacttttaga tgaacgaaga gcagaactca attttgaagg acatcgatat ttcgatttag
     7441 ctcactttgg tttagtggag tctgttttag gggaagaagt aatgccatgt ttcccaattc
     7501 ccggtcgtga aataaatgca agtggtggat tattaattca atatccggga tattaaatga
     7561 tacttaaggg aggaataata aaaatataat gaacgctttt aaatcgataa atccatttaa
     7621 tcaggaatta attgctgaac atccggtatt cacatcggag caaattgacg ctgctttgac
     7681 taatgcggaa aaagcatttc ataactggaa aacaacttca tttgaacagc gctccgattt
     7741 attgtttaaa ctttcaacag tattaaagaa taataaagag caatatgctt tacatattgc
     7801 gctggaaatg ggtaaaatat tagcggaagc aagagctgag gtagaaaaat gtgctttagc
     7861 atgtgaatat tatgcagaaa atggtccgat tttattacaa gatagaataa tcgaaagtga
     7921 tgcacgtaaa tcgttggttg cttatgaccc aatcggtgct gtgtttgctg tcatgccatg
     7981 gaattttcct ttttggcaag taatacgttt tgcagcgcca accattatgg caggaaatgt
     8041 ggcattactt aaacatgcta aaaatgtttc attatgtgcc aaagatattg aagctgcttt
     8101 tattgaagcc ggatttccta caggtgtttt tcaaagctta attgctcaag ctaatcaatc
     8161 ggaacaaata attgcgcatg atattgtgca gggtgtaaca ttaacaggta gcgaaaacgc
     8221 gggtgctgca gtggcattgc ttgccggtaa acatattaaa aaatcagtgc ttgaattagg
     8281 tggtagcgac ccatttattg tactcgatga tgcggatgtc cacgcagcag caaaaattgc
     8341 aacaaaaagc agaatgcaaa atgcagggca aagttgtatt gccgcaaaaa gatttatcgt
     8401 tacccaaaaa atagagcagg attttatcca ggcattcaaa aatgaaattg ctttaattca
     8461 tcagggcgac caattattag caacttcaac tatgggccct gtttcttcgg ttgtagcagc
     8521 tgatgaactt actacacaac aacaacaatc ggtggacctt ggcgcacaaa taattaccgg
     8581 tgggtttaaa aatggtgcaa ac
//
LOCUS       DAORKW010000093         8598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_217_length_8598_cov_4.822662, whole genome shotgun sequence.
ACCESSION   DAORKW010000093 DAORKW010000000
VERSION     DAORKW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217_length_8598_cov_4.822662"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1163
                     /locus_tag="PLB46_05465"
     CDS             6..1163
                     /locus_tag="PLB46_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRF75996.1"
                     /translation="MIHAIPVDELTGAISVPIYQTATYVQESPGIHKGYDYARSNNPT
                     RKALEDLIATLENGVAGFAFASGLAAIDAVLKLLKTGDEILAVDDIYGGAFRLFTHVY
                     EKFGIKVTYVDTTEIINVIDNINANTKLVWLESPTNPTLKISDIEAICNIAHQYGALV
                     VVDNTFASPVAQQPLLCGADIIVHSGTKYLAGHSDVIAGLVVTKTAALAEKIKFIQNA
                     SGGILGPFDSWLTIRGIETLPLRIEKSSTNALAVARFLSEHELVDKVYYPGLPTHKNH
                     LIATRQQQYFGGVVSFSLKDDEVSNANTFCSNTQLFKCAESLGGVKSLICHPASMTHK
                     SIPEQKRKQSGIQDSLIRLSCGIEAAADLIQDLHVAFEKSFSTIQKNKFIS"
     gene            1174..2388
                     /locus_tag="PLB46_05470"
     CDS             1174..2388
                     /locus_tag="PLB46_05470"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRF75997.1"
                     /translation="MQRKNLTIGVFGFGCVGQGLYDVLSRTQGLKVHIKKIGIKDATK
                     TRNLPAHYFTTNRDEILFDPEIDVVVELINDEHAAFEIVSTALRNGKAVVSASKKMIA
                     NNLKTLYALQQQYQVPLLYEGACCASIPIIRNLEEYYDNDLLNSVEGIFNGSTNYILT
                     KIFNENKSFSQALSEAQELGYAETDPSMDIEGYDPKFKLCILLLHAFGLFIQPEHIFN
                     FGINRINDFDINYAKEKHSSIKLLAKCRKVDNAVYAYVLPHFVNGNTQLTNIHNEYNG
                     VIVESAFADKQFFVGKGAGSTPTGSAVLSDISALSYDYRYEYRKTSQLLAKQAQNELK
                     LGDSLALDNNLHLKVYVRCKNQAIVPLTDFTEIIEKYESTEQQYIIGIINLATIMTSE
                     WVENEDINLIVL"
     gene            2600..3178
                     /locus_tag="PLB46_05475"
     CDS             2600..3178
                     /locus_tag="PLB46_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRF75998.1"
                     /translation="MIAISITDIITISLTLFAIIDVLGNVPVIISMKNKGIVLQPIKT
                     TIFAGVLMVTFLFLGDTILSLLGLDTQSFALAGSVVIFLLGLEMILGIHIFKSSPEGS
                     AAGSLVPIAFPLLVGAGTLTVLISMRSVYSIYDILIAVLINSIIMYFVLRSTTWIELK
                     IGKTALHAITKFFGVIALALAIKIFMTNFYHA"
     gene            complement(3251..4876)
                     /locus_tag="PLB46_05480"
     CDS             complement(3251..4876)
                     /locus_tag="PLB46_05480"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF75999.1"
                     /translation="MKPTFFVGLLLSIISLTTTAQTPNYLTMMSNGNVNENHMVVDRF
                     GNTYLTITYSGLIFIDGLDFYTDGPWTSLVLKFNHDGDLLWGYSFTGTGSITVQSIAN
                     DKGDGLAVAGNFNGSLNIDGETIVSQGLEEGFIAGFSPEGNIRYVNSFGTTLSDYAYS
                     ICVDKTGNKYILAEVHESFTFAGETIEPIGGVYPTVICKFNKLGAPTDLIAFNNDNYI
                     IYANMAIDNKNNLIISGRFNDHLSLLGTTMESAGFNYFAAKIDAGFNVVWTNVFPIGG
                     FTNIKDMVVDKAGNPYLLFVIDTLTIDGNTFYNAAMEDAYMESVVCKLNKSTGVAKWA
                     NKLNSFGEVYGTSLAVNGLQNVAVGGYYKDYINYGAMELTNPGPDLWDSFILMVDPDG
                     NALNLFDFGDNNTYDELWNIEFDNTGDMHLYGICSDGFSIGDFTAEVDFGAGYWLSAL
                     EFGTYKSEAIDLPATFTMSPNPAVNRVQLQFNANEVLDTPIQIIDITGKIVLTASLSN
                     ITGETITIDISSLAAGAYIVKYNQQQQQLIVTK"
     gene            5056..6570
                     /locus_tag="PLB46_05485"
     CDS             5056..6570
                     /locus_tag="PLB46_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76000.1"
                     /translation="MKPSDQLFRLIKALDTSEKGYFKKFAKGYGKAQNYILLFDAVDE
                     LNEYDENAVIKKLRKQPFIKQLAVTKNYLFELILKSQRQYKADISKFGKLNTLMENGE
                     ILFEKGLYEEALKTWEKAKTLALAFDEKPFILDIETNKRRYYIDMTACNWATYAEPSY
                     QNSFKLLSAYERMLKIQQLYVEIINVIKIQPFFRTQEQRSIWDKFMQDPLLAPENEPD
                     DFYGKLYFNYIYNIYHLLCRNKDEALIYIKKIVELWDSHQTLKDIEPIKYVSAVNNYL
                     TNLLFLGEKEQYIQYFDSIEWPNGNSISREAVIFEHYWLLKSSYYGLKNNITEAGQFF
                     VSTLPQIKKYARYINKVRLMIMYFSIAQYYTMVGNYVLSLDYLQLVQDSKEIELRKDV
                     QAMARLLILINHFESGNNMLMEHLTRSTKRFLQINNNYFETEKLFIKHLTLVLKSPDK
                     QSRQLAFVKMSEEIDSLFAANEQEKIALDSLRVSEWIKSKIANKPFGTYIESLV"
     gene            6748..7212
                     /locus_tag="PLB46_05490"
     CDS             6748..7212
                     /locus_tag="PLB46_05490"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRF76001.1"
                     /translation="MDSFKLDKTQTRIYIDKSERVLKIYANNQLLKTYPVVLGGNPKD
                     DKLQEGDNCTPEGKFAIRDLYPHKSWDKFIWVNYPTAASYVKHNDAKKQGHIKNNSGI
                     GGEIGIHGVPEGKDDWIDNNEDWTAGCISLKNEHIREIFASVQTGTEIEIVQ"
     gene            7240..8073
                     /locus_tag="PLB46_05495"
     CDS             7240..8073
                     /locus_tag="PLB46_05495"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HRF76002.1"
                     /translation="MIIISVSAISCKNKLLIKAGITKAPINEALIDTTGTTLQTRIKC
                     PEGYSRTVAEPNSFTSFIRNLKLKPHGTDVQLYNGKAKQVMVHCGVIDMPIRNKDLQQ
                     CADSGIRLYAEYLYATKQYDKIRFNFTNGQSCAYVKYAEGFRMRFEGEKAVWYPSKLK
                     DYGYETFLDYLDLVYMYAGSFSLSEELIKRPIQDIAPGDLFIYPGFPGHVVIVLDVCQ
                     NETTGEKLFLTAQGFTPAQVIEVLVNLESKHQNPWYTIPEANVITPQFDFTVDQLYRW
                     K"
     gene            complement(8123..>8598)
                     /locus_tag="PLB46_05500"
     CDS             complement(8123..>8598)
                     /locus_tag="PLB46_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76003.1"
                     /translation="GYVTTPGAVVADEAIQVSFKIDSLAHNDCACVAYSTVFTPSGVA
                     DQVALSNTACATLGTLARYGDDVVAEYLDNPTYFLNNQMLVYPNPSNGNFTLNLFEIE
                     DATITVVNTTGQVVYSTTGASDFVGITLENLTPGLYFVNAEYGNGKKITKSVVIE"
BASE COUNT         2897 a         1456 c         1562 g         2683 t
ORIGIN      
        1 caacaattat tcatgccatt ccggttgatg aacttaccgg agccatttca gttcccattt
       61 atcaaactgc aacgtatgtg caggaaagtc cgggcatcca taaaggatac gactacgctc
      121 gcagcaataa tccaaccaga aaagctttgg aagatttaat agcaacactg gaaaatggtg
      181 ttgcaggatt tgcttttgca agtggtttag cagccattga tgcagtttta aaattattaa
      241 aaaccggtga tgaaatttta gcagtggacg acatttatgg tggtgctttt cgtttgttta
      301 cacatgttta tgaaaagttt ggcatcaaag taacttatgt ggatacaacc gaaatcatta
      361 atgtaataga caacattaat gctaatacaa aacttgtttg gcttgaatca ccaaccaatc
      421 ctactttaaa aatttcggat attgaagcca tttgtaatat cgcacatcag tatggtgcat
      481 tggttgttgt agataatacg tttgcctcgc cggtcgctca acaaccgttg ttatgcggtg
      541 cggatattat tgtgcattct ggcacaaaat atctggcagg acatagtgat gtaattgcag
      601 gattagtagt aacgaaaaca gctgccttag ctgaaaaaat caagtttata caaaatgcca
      661 gtggtggtat tttaggtccg tttgacagtt ggttaactat cagaggcatt gaaacattgc
      721 cattacgcat cgaaaagtca agtaccaatg cgctcgctgt tgcacgattt ttaagcgaac
      781 acgaattagt ggataaggta tattatccgg gtttgccaac acataaaaat catttgatag
      841 ccacgcggca gcaacagtat tttggaggtg tggtgagttt tagtttaaaa gatgatgaag
      901 tatccaatgc gaatacattt tgtagcaata cccaattatt taaatgtgca gaaagtttag
      961 gcggcgtaaa aagtttaatt tgtcatccgg caagtatgac ccataaatca attcctgaac
     1021 aaaaacgaaa acaatccggg attcaggatt cattaattag gttatcctgc ggaattgaag
     1081 cggctgcaga tttaattcag gatttacatg tagcttttga aaagagtttc agcacaatac
     1141 aaaaaaataa attcatctct taaacaaact atcatgcaaa ggaaaaattt aaccataggt
     1201 gtatttggat ttggttgtgt tggtcaaggg ttatacgatg tattaagtcg aacacaaggg
     1261 ctaaaagtac atatcaaaaa aataggaata aaagatgcga ccaaaacacg caatttacct
     1321 gcccattatt tcaccaccaa cagagatgaa attttgtttg atccggaaat agatgtagtt
     1381 gtagaattaa ttaatgatga acatgctgca tttgaaattg tttcaactgc cctacgtaat
     1441 ggaaaagcag ttgtgagtgc aagtaaaaaa atgattgcca ataatttaaa aactttatat
     1501 gccttgcaac aacagtatca ggtgccacta ctttatgaag gtgcatgttg tgcgagtata
     1561 cccattatca gaaacctgga agaatattat gacaacgatt tattaaacag cgtagagggt
     1621 atttttaatg gttcaacgaa ttatatctta accaaaatat ttaatgaaaa taaaagtttt
     1681 agtcaggctt taagtgaagc tcaggaatta ggttatgcag aaacagaccc gtcaatggac
     1741 attgaaggtt atgaccccaa gtttaagctg tgtattttat tattacatgc atttgggttg
     1801 tttattcaac cggagcacat atttaacttt ggcatcaaca gaattaatga ttttgatatt
     1861 aattatgcca aagaaaaaca tagtagtata aagttgttag caaaatgccg aaaagtagat
     1921 aacgccgttt acgcctatgt gttgcctcat tttgtaaatg gcaataccca actaaccaat
     1981 attcacaatg aatacaatgg ggttattgtt gaaagtgcat ttgcggataa acaatttttt
     2041 gtaggcaaag gcgcaggaag cacaccaacc ggttctgcag ttttgagcga tatttctgca
     2101 ttgagttatg attatcggta tgaatatcgc aaaacaagtc agctgcttgc caaacaggct
     2161 caaaatgaat taaaacttgg agattcatta gcactcgata acaatttaca tttaaaagta
     2221 tatgtgcgtt gcaaaaacca ggcaattgtt cctttaactg acttcacaga aattatagaa
     2281 aaatacgaat caacggaaca gcagtatatc ataggaataa tcaatctggc taccattatg
     2341 acatcagaat gggttgaaaa cgaggatatc aacctgattg tgctataaaa ctgacattat
     2401 tttgaaaatc gggaactttt aagtcccgat tttccttcat acctttgtta cggcgatgaa
     2461 attcacatta aatatctaca acaatgctat tgggattcat gagtatccct ggctttcgtc
     2521 tgcattagct tgctgctaat tatagtattc ctgtttacac accaatccac atttttatta
     2581 ccttttttaa gcttttgtta tgattgctat tagcattact gatattatta ccattagttt
     2641 aaccctattt gccattatcg atgtgttggg taatgttcct gttattattt ctatgaaaaa
     2701 taaaggaatt gtactacaac ctattaagac aaccattttt gccggtgttt taatggttac
     2761 ctttttattt ttaggtgaca ccattttatc cttgcttggc ctcgataccc aatcgtttgc
     2821 attagcaggc tcagttgtta tttttttatt gggattagaa atgattttag gtattcatat
     2881 ttttaagtcg agccctgaag gttctgctgc tggtagttta gttccgattg catttccatt
     2941 attagttggt gcaggtactt taactgtttt aattagtatg cgcagtgtgt attctattta
     3001 tgatatatta attgcagtac ttattaattc aattatcatg tactttgtat tacgttcaac
     3061 aacctggatt gaattaaaaa tcggcaaaac tgcattacat gccattacta aattttttgg
     3121 tgttattgca ttggctttag cgattaaaat ttttatgacc aatttttatc atgcataaaa
     3181 aagttcctct taaaaaatga agaggaactt taattacgga gttgaaataa acaccatatt
     3241 tgaaacctaa ttatttggtt acaataagtt gttgttgttg ttgattgtat tttacaatat
     3301 atgctcctgc agccaatgat gaaatgtcta tagttatagt ttcgccggtg atattactca
     3361 ggcttgccgt aagtactatt ttcccggtaa tatctattat ttgtattgga gtatctaaaa
     3421 cttcgtttgc attaaattgt aattgcacac gatttacagc aggatttggg ctcattgtaa
     3481 atgttgcagg taaatcgata gcctcagatt tataggtacc gaattccaaa gcggacagcc
     3541 agtatccggc tccaaagtcc acttctgcag taaaatcacc aatggaaaat ccgtcgctac
     3601 atattccgta tagatgcata tcaccggtat tatcaaattc aatattccat agttcatcat
     3661 aagtattgtt gtcgccgaaa tcaaatagat tcaatgcgtt accatcaggg tcaaccatta
     3721 atataaaact atcccataaa tcggggccag gattagttaa ttccatagca ccataattga
     3781 tataatcttt ataatagccg cctacagcaa cattttgcaa accattaact gcaagtgaag
     3841 ttccatatac ttcgccaaaa ctgtttaatt tattagccca ttttgccacg ccggttgatt
     3901 tatttaattt acaaactaca ctttccatgt atgcatcttc cattgcagcg ttgtaaaaag
     3961 tatttccatc aattgtcagg gtatcaatta caaataataa ataaggatta ccggctttgt
     4021 caactaccat atcttttata ttggtaaaac cgccaattgg gaacacattt gtccatacca
     4081 cattaaatcc ggcatctatt tttgccgcaa aataattaaa gccagcactt tccattgttg
     4141 tgcccagtaa tgataaatgg tcgttaaatc ttccactaat aatcaggtta tttttattgt
     4201 caatggccat gttagcatat atgatataat tatcgttatt aaatgcaatt aaatcggtgg
     4261 gagcacccaa tttattgaac ttacaaatta ctgtaggata tacaccacca attggttcaa
     4321 tagtttcacc ggcaaatgta aatgattcgt gcacttctgc cagtatatat ttattccctg
     4381 ttttatcaac gcaaatacta taggcataat cacttaatgt tgtaccaaaa ctatttacat
     4441 accgaatatt gccctctggt gaaaagccgg caataaatcc ttcttccagg ccctgtgaaa
     4501 caattgtttc accatcaata tttaaggaac cattaaaatt accggctact gctaagccgt
     4561 cgcctttatc gttcgcaata ctctgaaccg taatggaacc tgtaccggtg aaggaataac
     4621 cccaaagtaa atctccatcg tgattaaatt ttaaaaccag tgacgtccaa ggaccatcgg
     4681 tataaaaatc caatccgtca ataaatatta atccgctata agtaatggtt aaatacgtgt
     4741 tgccgaatcg gtcaaccacc atgtggtttt catttacatt gccattactc atcatggtca
     4801 gataattggg agtttgtgct gttgtcgtca ggctgattat acttaataat aagccaacaa
     4861 aaaatgtagg tttcattttg atattcaatt ttgtgttaaa taatggtgca aacctattgc
     4921 ggcgtagcag gtggccaaaa caaaaaaagg agttattatt aggttccggc aggttgctaa
     4981 aaagctacaa gtattgcctg caaagggttg tggccaaaat ggcgtatcga cgattttgct
     5041 taaatttggg cgttcatgaa gccttcagac caattatttc gtttaattaa agcgctggat
     5101 accagtgaaa aagggtattt taaaaaattt gccaaggggt atggcaaggc ccagaattat
     5161 attcttttgt ttgatgccgt tgatgaacta aacgagtatg atgaaaatgc tgtaattaaa
     5221 aaattacgca aacaaccatt cataaagcaa cttgccgtta caaaaaacta tttatttgaa
     5281 cttatattaa agtcgcaacg acaatacaaa gccgatattt caaagttcgg taaattgaac
     5341 accctgatgg aaaatggtga aatattattt gaaaaagggc tgtatgagga agcacttaaa
     5401 acctgggaga aagccaaaac cttagctctg gcgtttgatg aaaagccatt tattttagat
     5461 atcgaaacca acaaacgcag atattatatc gatatgactg cctgtaattg ggcaacatac
     5521 gcagagccta gttatcagaa tagttttaag ttactgtcgg catacgaaag gatgttaaaa
     5581 atccagcagc tatatgttga gattattaat gtaataaaaa ttcaaccttt tttcagaact
     5641 caggaacaac gaagcatttg ggacaaattt atgcaagacc ccttattggc accggaaaat
     5701 gagcctgatg acttttatgg caagttatac tttaattata tctacaatat ttaccattta
     5761 ctttgccgta ataaggatga agcactcatt tatataaaaa aaattgtaga attatgggat
     5821 agtcaccaaa cgttaaaaga tattgaacct ataaaatacg tttcggcagt aaataattat
     5881 ttaaccaatt tattattcct tggggaaaag gaacaatata ttcaatactt tgattctatt
     5941 gaatggccga atggaaattc tatttcacgg gaagccgtta tatttgagca ttattggtta
     6001 ttaaaaagta gctattacgg cctaaagaat aacatcactg aagcaggcca atttttcgtg
     6061 tctaccttac ctcagattaa gaaatatgcc cggtatatca ataaagtgcg tttgatgata
     6121 atgtactttt caattgcgca atattatacc atggtaggca attatgtcct atcattggat
     6181 tatttgcaac ttgttcaaga ctccaaagaa atagaattac gtaaagatgt tcaggcaatg
     6241 gcccgattgc ttatacttat aaaccatttt gaatcgggta acaatatgtt aatggaacat
     6301 cttacacgtt ctactaaacg attccttcaa ataaataata attacttcga aactgaaaag
     6361 ttgtttatta aacatctaac attggtatta aagtctccgg acaaacaatc caggcaactg
     6421 gcttttgtaa aaatgtcaga agaaattgat tctttgtttg cagccaatga gcaggaaaaa
     6481 attgcattag acagtttacg tgttagtgaa tggattaagt cgaaaattgc gaacaaacca
     6541 tttggcacct atatagaatc attagtttag gttcaaaaat taaaataaat taccggaatt
     6601 ttcgttttag catccatgaa aaccgcggtt gccattattt tattcttcgt atgtaatgct
     6661 tgcaaaccat tcctaccgaa aaatgagcat tttcaaaatg aaactttaca aagaattgca
     6721 aacccggaaa ttccattagc gcaaattatg gattcattta agttggataa aacacaaacc
     6781 cgtatttata ttgataaatc ggagcgggta ttaaaaattt atgctaacaa tcaactgctt
     6841 aaaacctatc cggtagtttt gggaggtaac ccaaaagatg ataaattaca agaaggagac
     6901 aactgcacac cggaaggcaa gtttgctata agagatttat atccgcataa atcctgggat
     6961 aaatttattt gggtaaatta tccaacggca gcaagttatg tgaaacataa tgacgccaaa
     7021 aaacaaggac atattaaaaa taattcaggt ataggcggag aaattggtat tcatggtgta
     7081 cctgaaggaa aagatgattg gatagataat aatgaggatt ggacagcggg gtgtatctca
     7141 ttaaagaatg aacatatcag agaaattttt gcatctgttc aaaccgggac tgaaattgaa
     7201 attgtgcaat aatatcacat gaaaataaaa ctgctgttaa tgattataat cagcgtatca
     7261 gctatttcct gtaaaaataa attgctgata aaagcgggta ttacaaaggc accaattaat
     7321 gaagcgttaa tagacactac cggaactacc ttgcaaacca ggataaaatg tcctgaaggt
     7381 tattcaagaa cagtagccga acctaatagt tttacctcat ttataaggaa tttaaaatta
     7441 aagccacatg gtacggatgt tcaattgtat aatggtaagg ccaaacaggt tatggtgcat
     7501 tgcggagtta tagatatgcc tatacgcaat aaagatttac aacaatgcgc cgatagcgga
     7561 attaggcttt atgcggaata tttatatgcc actaaacaat acgacaaaat acgttttaat
     7621 ttcacaaacg gacaatcatg tgcatacgta aaatatgccg aaggttttag aatgcgattt
     7681 gagggtgaaa aggccgtgtg gtatccctcc aaattaaagg attacggcta cgaaacattt
     7741 ttagattatt tagatttggt atatatgtat gccggttcat tttcgctatc tgaagaactc
     7801 ataaaaagac cgattcagga tatagcgccc ggcgatttat tcatatatcc cggatttccc
     7861 gggcatgtag ttatcgtatt agatgtttgc caaaatgaaa ctacaggtga aaaattattt
     7921 cttaccgcac agggatttac accggcgcag gtaattgaag tgttggtaaa tttagaatca
     7981 aaacatcaga acccgtggta tactattccg gaagcaaatg ttattacacc acaatttgat
     8041 tttactgttg atcagttata cagatggaaa taaaaaagcg cattcatttt tatgaatgcg
     8101 ctttaaaaaa gttaatcgga tattattcga taactactga tttagtaatt tttttaccat
     8161 taccatattc agcatttaca aagtataaac ccggagttaa attttcgagt gtgataccaa
     8221 cgaaatcgct agcacctgta gttgaataaa ctacttgtcc tgttgtattt acaacagtaa
     8281 ttgtagcatc ttcaatttcg aataaattta aagtgaagtt accgttagat gggtttggat
     8341 aaaccaacat ttgattattt aagaaataag ttgggttatc taaatattca gcaactacat
     8401 catcgccgta acgagcaagt gttcctaaag tagcgcaagc agtattactt aatgcaacct
     8461 gatcggcaac acctgaaggt gtaaatacgg tgctataagc aacacaagca cagtcgttat
     8521 gagcaagtga atcgattttg aaagatacct gaatggcttc atcggctaca actgcaccag
     8581 gggtagtaac ataaccac
//
LOCUS       DAORKW010000094         8522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_222_length_8522_cov_5.059053, whole genome shotgun sequence.
ACCESSION   DAORKW010000094 DAORKW010000000
VERSION     DAORKW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222_length_8522_cov_5.059053"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLB46_05505"
     CDS             complement(<1..213)
                     /locus_tag="PLB46_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF76004.1"
                     /translation="MLKCCILDDEPLAIKLLSGYVAKTPLLELTLATTDPFKALATVQ
                     SGEVDLILLDIQMPELNGYEASIKIRE"
     gene            complement(216..1055)
                     /locus_tag="PLB46_05510"
     CDS             complement(216..1055)
                     /locus_tag="PLB46_05510"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF76005.1"
                     /translation="MLKTYPKKLSQLIISLILLIPAFIAFRYLLEETLCPILIGRSNY
                     VETTTVEYYIADNFSYALYYMVFGILLFMLDYNMLNNKKVKLLESQNREAQLQYLQTQ
                     FNPHFLFNSLNNIYSLVTEKSDNAGEAVMRLSDMTRYLIYNNSNECLLKDELIQVDNY
                     INLQKLRFDYELPIIYELSGNYESKQLPTFTVVTFIENAFKHGDFKEKEKPLFIGVDA
                     GNNILHIIVKNKIGAYNKDKTSGVGLDNIRKRLAIIYPNQHQLICSVENNISTVDLTI
                     NYY"
     gene            1401..2291
                     /locus_tag="PLB46_05515"
     CDS             1401..2291
                     /locus_tag="PLB46_05515"
                     /inference="COORDINATES: protein motif:HMM:NF039197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HRF76006.1"
                     /translation="MLLKGITFSICLSIMMLSVNAQHRLTVEQAKTQSDSILNGDRNL
                     PQMMVLGSFHFAYYNLDAHVTSEDEQVDVLSAQRQKEMEELVAYISKYKPTKIAVESG
                     PITGYLLRNYEEWIAGKRSLGRSETEQIGFRLMRQFGMDTIYGVDAIPLMFDLYDAKD
                     SAVLRPLLDSIYTDWDFRSDDSISKLYSDWYNFDDQLALKMSLKDYFIYMNDDWVLDK
                     GYGAYLTGDFKLGSFEGADALAMHWYSRNLRIFRNLQEIVTSPDDRILLIIGAGHAGI
                     LTHLFECSNEFELVKVGEIK"
     gene            complement(2350..4221)
                     /gene="htpG"
                     /locus_tag="PLB46_05520"
     CDS             complement(2350..4221)
                     /gene="htpG"
                     /locus_tag="PLB46_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRF76007.1"
                     /translation="MSIEKGTIAVQTENIFPIIKKFLYSDHEIFLRELVSNAVDATQK
                     LKKLSSLGEISGEIGSTNISISIDKDAGTLTISDKGIGMTAEEVKKYINQVAFSSASE
                     FLEKYKGLDDGKSIIGHFGLGFYSAFMVADKVMLVTKSGKENAEPVRWTCDGSTEFTL
                     ETWEKAERGTDVILFINEENKSFLEDAKIEGLLNKYCKFLPVEIQFGVKEETVKGDED
                     GTDTTVTNPNIINNPSPAWTKKPAELQDEDYKNFYNELYPYTEAPLFWIHLNVDYPFN
                     LTGILYFPRIKKSYEIQKNKIQLYSNQVFVTDEVKEIVPEWLTLLHGVIDSPDIPLNV
                     SRSYLQSDQNVKKINNYITKKVAEKLEETFKKDRAEFENKWENINVIIKYGMLSDEKF
                     AERAEKFCLLQNTDKKYFTFEEYKEHIKAGQTDKNNNIIALYTNNTTDHHVYVDAAKG
                     RGYDVLETDNVIDNHFISFMEQKMTGVQFKRVDADTVDKLIDKDEKTESILSEEESNS
                     LKELFEKIADANAATIVLSPQSPDDNVVAITKPEFMRRMKEMSHLNGMDFAASMPDQY
                     NLVVNTNHPVIQKILKQEETVKAQSVKQLHDLALLSQGMLKGTDLTEFVKRSVELMN"
     gene            4355..>4854
                     /locus_tag="PLB46_05525"
     CDS             4355..>4854
                     /locus_tag="PLB46_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76008.1"
                     /translation="MAGLNRTYIQKTKAEIEELLHTSWDLVSKRNSEIENYSKKALKL
                     ADSIDSNNYRGLAIIESALFECLVKNNYYQSIKLCTEGFDLMKGEFKKRYAPYFHLNL
                     GRNYHFLGDNVLTQKHYLETIKLLEHRPDKNYYEKRWLSHTYYNIFILFNFSGAEFAQ
                     SEYLEKA"
     assembly_gap    4855..4954
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4955..6022
                     /locus_tag="PLB46_05530"
     CDS             <4955..6022
                     /locus_tag="PLB46_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF76009.1"
                     /translation="DREINSTYHSAHTHNQMGEAHAIQKNYQKAIEHFKVAEKYFESV
                     GVKKSLSTLYDNVSLAYLGMGDFENAYTYQKKYSDSLKDLFNDEKTFAIARARNEYEL
                     EIKEQEAQILRSKNEQIAKYANQLEFSNNELRQFAHVASHDLREPLRMVSSYVQLLER
                     SLKNKLTDDEKDFMRFIVTGTHTMQNLISDLLTLSGISFVRSASPVDLNNVMKTVVST
                     LKTQIEEKQVIINYSLLPTVKGDETHMMQLFQNLISNGIKYNKSEPPIVDISFNLTGK
                     KYHFAVSDNGIGIPDEFVEKIFLIFQRLHSRDEYSGTGIGLAICKKIIDQAGGKIWVE
                     PNKGGGCVFKFTLPVSQGNNV"
     gene            6063..6542
                     /locus_tag="PLB46_05535"
     CDS             6063..6542
                     /locus_tag="PLB46_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HRF76010.1"
                     /translation="MKLKISYFFLIIGLTFVFTSCATSKEMEYKGFENFAVSGITSQP
                     KMNVDVTLYNPNKFGATLKTMEFTVLVNDKPMGSGGILEPIKVKKQTEFILPVECSTS
                     LDKMGGLLAAGLKSYLGEEKVPVGLEGAITVQKFYFFRKTYSFKFTDELDIKKIMGN"
     gene            complement(6539..7066)
                     /locus_tag="PLB46_05540"
     CDS             complement(6539..7066)
                     /locus_tag="PLB46_05540"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HRF76011.1"
                     /translation="MAANPDTQNPASPLLWHVAYVKSRSEKKVKERLDRMGIQAFLPL
                     IKTVRQWSDRKKTVQVPLFNGYIFLQLSGDDYTRVRMVEGVVNFIYQEKKIAVVPEKQ
                     IEDIRTFLESGLPVSASTDTFSKGEQVVINFGPLKGYQGELLEIENENHFIVRIEVIN
                     QILRLSVPKAYLEKV"
     gene            complement(7073..8110)
                     /locus_tag="PLB46_05545"
     CDS             complement(7073..8110)
                     /locus_tag="PLB46_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF76012.1"
                     /translation="MIENKINCAVIGCGHIGKRHAEMILRNNRTQLVALCDIQPKEEL
                     NIDKFPGKFYSDIQQLLSEEKNIDVVHICTPNGLHATHANLALLAGKHVVIEKPMALT
                     VADCDSIIANAAIHNKSIFCVMQNRFSPPSVWLKNMITENRLGQIYMVQVNCFWNRDE
                     RYYHKNTWHGKNELDGGTLFTQFSHFIDTMVWLFGDIENIQTQFADFNHQSLTDFEDS
                     GNITFSFKNGGIGSMHYSSSVYQQNFESSIRIIGEKGTIAVGGQYMNEVKYCHVENYT
                     MPEIQPTAAPNNYGPYIGSAANHHFIIDNIVDVLDNNGAIATTAEEGKLVVSVIERIY
                     AQRPDYLLKNK"
     gene            complement(8114..>8522)
                     /locus_tag="PLB46_05550"
     CDS             complement(8114..>8522)
                     /locus_tag="PLB46_05550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRF76013.1"
                     /translation="NVKLYYDVSNYSGVVCEDDVFLGPSMVFTNVINPRSAIERKNEF
                     KSTLVKKGATIGANATIVCGITIGAYAFIGAGAVVTKSIDDYTLVTGNPAKHQGWMSE
                     HGAKLIFDENGKATCAISGASYQLINNRVSKIG"
BASE COUNT         2630 a         1408 c         1536 g         2848 t
ORIGIN      
        1 ttccctgatt tttatactcg cttcatatcc atttaattcg ggcatttgaa tgtcaagtaa
       61 aattaaatca acttctcccg actgcactgt tgctaaagct ttaaatgggt cggttgtggc
      121 aagtgttaat tccagcaatg gcgttttagc aacataacct gacaataatt ttattgcgag
      181 cggttcatcg tctaatatgc aacacttaag cattgttaat aataattaat tgttaaatct
      241 acggtagaaa tattgttttc gaccgaacaa ataagttgat gttggttagg gtaaataatg
      301 gccagtcgtt ttctgatgtt gtctaggcca actccgctgg ttttgtcttt attgtaggcg
      361 ccaattttat ttttgacaat aatatgtaaa atgttgttgc cggcatcaac acctataaac
      421 aaaggttttt ctttttcttt aaaatcgccg tgtttaaatg cgttttcgat aaaagttact
      481 acggtaaaag taggtagttg tttggattcg taattgccgg atagctcata aataatgggt
      541 aattcgtagt caaacctgag cttttgaaga ttgatataat tgtccacttg aataagttca
      601 tctttcaata aacactcatt tgagttgtta taaattaaat atcttgtcat atctgaaagc
      661 cgcatcactg cttcgcctgc gttgtcggat ttttctgtaa cgagggaata gatattgttt
      721 aaactattaa ataaaaaatg gggattaaat tgcgtttgta aatattgtag ttgggcttcg
      781 cgattttgag attcgagtaa tttcactttt ttattgttga gcatattata atctaacata
      841 aacaataaaa taccgaacac catgtaatac aaagcataac taaaattgtc ggcaatataa
      901 tattcaactg tggtagtctc cacataatta cttctaccta ttaaaatggg gcataaggtt
      961 tcttctaata aataccggaa tgctatgaaa gccggaatta gcaaaatgag tgatatgata
     1021 agctgagata attttttagg atatgttttt agcattatta agcgcactga caaaactgtc
     1081 gctgtgatat agataaaagc ggtaatgtca aatggagtga acctaagcgc tcgactataa
     1141 tcacgttgaa ggattccata atcaataaag ttaaagccga ataacagcat gaaaatggtt
     1201 aagccatagg caaaccagag ttgtgatttc gtcatgtgat aaaggtattt aaagaaacca
     1261 tgataatttg tgtttgtctg caaacaccta caatttgtct gcaaacataa tttacgcgtt
     1321 tgcccgataa aataaggtgt tgggcgaggg tagtagtaag cgcgtatttg acattttact
     1381 tttgaggaaa actatacatt atgctattaa aaggaatcac gttttcaatt tgtttaagta
     1441 taatgatgtt gtctgtaaat gcacaacatc gtttaactgt agaacaagca aaaacacaat
     1501 cggacagtat tttaaacggc gacagaaact tgcctcagat gatggttttg gggagtttcc
     1561 atttcgcgta ttacaatttg gatgcgcacg taacgagcga agatgaacag gtggatgttt
     1621 tatctgcaca acgtcaaaaa gaaatggaag aattagttgc gtatatcagt aagtacaaac
     1681 caactaaaat tgctgttgag tcaggaccaa ttaccggtta tttattgcgt aattatgaag
     1741 aatggatagc aggtaaacgt tcgttgggta gaagtgaaac tgaacaaatt gggttcagac
     1801 taatgcgaca atttggaatg gacaccattt atggtgtaga tgcaataccg ttgatgtttg
     1861 atttatatga tgctaaagat tctgcagtac ttcgcccatt attggatagc atttataccg
     1921 attgggattt tagaagtgat gatagtattt cgaaacttta tagtgattgg tataattttg
     1981 atgaccaatt ggcattaaaa atgtcactca aagattattt tatttatatg aatgatgatt
     2041 gggttttgga taaaggttat ggcgcatatc ttacgggcga ctttaagtta ggctcatttg
     2101 aaggtgcaga tgcgttggct atgcattggt atagcaggaa cctccgaatt ttccgtaatc
     2161 tgcaggaaat tgttacaagt cctgatgata gaattttatt aattattggt gcaggccatg
     2221 cgggtattct gactcatttg tttgagtgtt caaatgaatt tgaattagta aaagttgggg
     2281 aaataaaata gccaggtaac cggacataaa aaaatccgct taaagcggat ttttttatgt
     2341 tgtgtattat taattcatta attcgacgct gcgcttaaca aattcggtaa ggtcggttcc
     2401 ttttagcatg ccttgagaaa gtaatgcaag gtcgtgaagt tgttttaccg attgcgcttt
     2461 aactgtttct tcctgtttta atattttttg aataacagga tggttagtat ttactaccag
     2521 attatattgg tctggcattg atgctgcgaa atccattcca tttaaatggc tcatttcttt
     2581 catcctgcgc ataaattccg gtttggtaat tgcgacaaca ttatcatcag gcgattgtgg
     2641 agaaagaaca attgtggctg cattggcgtc agcaattttt tcaaataatt cttttaagga
     2701 attagattct tcttcactta gtatagattc tgttttttcg tctttatcga taagtttgtc
     2761 aacagtatcc gcatctacac gtttaaactg aacgccggtc attttttgtt ccataaagga
     2821 aataaaatga ttgtctataa cattgtcggt ttctaaaaca tcgtaaccac ggccttttgc
     2881 tgcatctaca taaacatggt ggtcggtggt attattggtg tagagagcaa taatattatt
     2941 gtttttatct gtctgtccgg ctttgatatg ttctttgtat tcttcgaatg taaaatactt
     3001 tttatcggta ttttgtaata agcagaattt ttcagcgcgt tcagcaaatt tttcatcgct
     3061 taacataccg tatttaataa taacattgat gttttcccac ttgttttcaa attcagccct
     3121 gtccttttta aatgtttctt ccagtttttc agccaccttt ttggtgatgt aattattgat
     3181 tttttttaca ttttggtcgc tttgcaaata acttcttgac acgtttaacg gaatgtccgg
     3241 cgaatcgata acaccatgta aaagggttaa ccattcgggc acaatttctt ttacctcatc
     3301 ggtaacaaaa acctgattac tataaagttg gattttattt ttttggattt cgtagctctt
     3361 tttaattcga gggaaatata aaataccggt taggttaaat ggataatcaa catttaaatg
     3421 aatccaaaat aaaggtgctt cagtataagg gtataattcg ttgtaaaagt ttttataatc
     3481 ctcatcttgt aactctgccg gtttttttgt ccatgccgga gatgggttat taataatatt
     3541 aggattggtt acagtggtat cagttccatc ctcgtcacct ttaacggttt cttctttaac
     3601 accaaattga atttcaacag gtaaaaattt acaatatttg ttgagcaggc cttcaatttt
     3661 agcatcttca agaaacgact tgttttcctc atttataaat aaaataacat ctgtccctct
     3721 ttccgctttt tcccatgttt caagtgtaaa ttcggtactg ccatcacaag tccagcgaac
     3781 aggctctgca ttttcttttc ccgactttgt aacaagcata accttatcgg caaccataaa
     3841 tgcgctataa aaacctaatc cgaaatgtcc gataatactt ttaccatcat ccaggccttt
     3901 atatttttct aaaaactcgc tggcactgga aaaagctacc tgattgatat attttttcac
     3961 ctcctctgcg gtcatcccga tacctttatc ggaaatagtt agggtgcctg catctttgtc
     4021 gatactgata ctgatattgg ttgaaccaat ttcaccggaa atttcaccta gtgaggatag
     4081 tttttttaac ttttgggtag catcaacagc atttgatacc aattcgcgta agaatatttc
     4141 atggtcgctg tataagaatt ttttaataat tgggaaaatg ttttccgttt gaacagctat
     4201 ggttcctttt tctattgaca tatttaggta attttaagta agaccccctt ttcaaacctt
     4261 gtgccatgag gttaaggctg acaatttgtc gctgaattca agcgttttta gctggaattg
     4321 gctaaattta cccccttgtt gataggaaca gataatggca ggattaaatc gcacatatat
     4381 tcaaaaaact aaagcggaga ttgaggagtt attgcatacg tcctgggatt tggtgtcgaa
     4441 acgcaattct gaaatcgaaa actattcgaa aaaggcttta aaattagctg attcgataga
     4501 ctcaaataat tacagagggt tggccattat tgaatccgct ttgtttgagt gtttggttaa
     4561 aaataattac tatcaatcta tcaaactttg cacagaaggg ttcgacctga tgaaaggcga
     4621 atttaaaaag cgatatgctc cctatttcca tttaaattta ggcaggaatt atcatttttt
     4681 aggtgataat gttttaaccc aaaaacatta tctggaaacc attaaattat tagaacatcg
     4741 tccggataaa aattattatg aaaaacgatg gctgtcgcat acctattaca atatttttat
     4801 tttatttaat ttttccggag ctgaatttgc tcaaagtgag tatttggaaa aagcnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaccgc gaaataaaca gcacttacca
     4981 ttcggcgcat acgcataacc aaatgggtga agcacatgct attcaaaaaa attaccaaaa
     5041 agccatcgag cattttaaag tagcagaaaa gtattttgaa tcagttgggg taaagaagtc
     5101 gttatccact ttatacgata atgtttcctt agcatattta ggtatgggcg attttgaaaa
     5161 tgcgtatact tatcaaaaga aatattccga ttcattaaaa gatttattta acgatgaaaa
     5221 aacgtttgca atagcgcgtg cacgaaacga atatgaattg gaaattaaag agcaggaagc
     5281 acaaattttg cgttctaaaa atgagcagat tgccaaatat gcgaatcaat tagaattttc
     5341 caataatgag ttacgccaat tcgctcatgt tgccagtcac gatttacgag agccactacg
     5401 tatggtttcg tcgtatgtgc aattattgga aaggagttta aaaaataaac tcaccgatga
     5461 tgaaaaagat tttatgcggt ttattgtaac gggaactcat accatgcaaa acctcataag
     5521 tgatttgtta accttgtctg gtatcagctt tgttcgatca gcaagccctg ttgatttaaa
     5581 taatgttatg aaaacggttg tttcaaccct caaaactcaa attgaagaga agcaggtaat
     5641 aatcaactat tcattacttc ccacggtaaa aggtgatgaa acgcatatga tgcaattatt
     5701 tcaaaacctg atatccaacg ggataaaata caataaaagt gaaccaccta ttgtagatat
     5761 ttcttttaat cttaccggaa aaaaatatca ttttgctgtt tcagataatg gtattggcat
     5821 acctgatgaa tttgtcgaaa aaatattcct cattttccag cgtttacatt ctcgtgatga
     5881 gtattcagga acgggcatcg gactggcaat ttgtaaaaaa ataattgacc aggcaggtgg
     5941 aaaaatatgg gtggaaccaa ataaaggcgg cggatgtgtg tttaaattca ccttaccggt
     6001 ttctcagggt aataatgttt aatgccatta aaaatgggca tacttgttgt aatatagtgt
     6061 atatgaaatt gaaaatcagt tacttttttt taattatagg gttaacattt gttttcactt
     6121 cttgcgcaac ctccaaggaa atggaatata aaggatttga aaattttgcg gttagtggaa
     6181 ttacttctca gcctaaaatg aatgttgatg tgacacttta taatcctaat aaattcggtg
     6241 ctacacttaa aacaatggag tttactgttt tagttaatga caagccgatg ggaagtggtg
     6301 gcattttaga acctattaaa gtgaaaaaac aaacggaatt tattttgccg gttgagtgtt
     6361 ctacatcatt agataaaatg ggtggactgt tagcagccgg attaaaatcc tatttaggtg
     6421 aagaaaaagt gccagtagga ttggaaggtg cgataacagt gcagaaattt tatttcttca
     6481 gaaagaccta tagttttaag tttaccgatg agcttgatat taaaaaaata atgggtaatt
     6541 aaactttttc gagataagct ttagggacag ataatcgtaa aatctgattg attacttcga
     6601 tacgcacaat aaaatgattt tcattttcta tttccagtaa ttcgccctgg tatcctttca
     6661 acggaccgaa attaataaca acctgttcgc cttttgaaaa agtatcagtt gaagcagata
     6721 cgggtaatcc agattctaaa aaagtgcgaa tatcttcgat ttgtttttca gggactaccg
     6781 caattttttt ctcctgatat ataaaattaa caacaccttc caccatacgc actctggtat
     6841 aatcgtcgcc tgatagttgt aaaaaaatat aaccattaaa cagcggaacc tgaacagttt
     6901 tttttctatc gctccattga cgtaccgttt taattaatgg aaggaaagcc tgaataccca
     6961 tgcggtctaa tcgttctttt actttttttt cgctgcgtga ttttacatat gcaacatgcc
     7021 aaagcaaagg agaggctgga ttttgtgtat cgggattggc tgccatttac aattatttat
     7081 tttttaataa ataatcaggt cgttgcgcgt aaattctttc gattacactc acaactaatt
     7141 tgccttcttc agcagtagtg gcaattgcgc cattattatc gagtacatct acaatattat
     7201 caataataaa atgatgattt gcagctgagc cgatatacgg gccataattg tttggagcgg
     7261 cagtaggttg tatttcaggc atggtataat tttcaacatg acaatattta acttcattca
     7321 tatattgtcc gccaactgca attgttcctt tttcacctat gatgcgaata ctgctttcga
     7381 agttttgttg atacactgac gatgaataat gcatactgcc aattccgcca tttttaaatg
     7441 aaaaagtaat atttcctgaa tcttcgaaat cggttaatga ttgatgatta aaatccgcaa
     7501 attgtgtttg aatattttct atatcaccaa acagccaaac catggtgtcg atgaaatgtg
     7561 aaaattgcgt aaataatgta cctccgtcta attcattttt cccatgccag gtgtttttgt
     7621 ggtaataacg ttcatcgcga ttccaaaaac aatttacctg caccatataa atttgtccca
     7681 atcggttttc tgtaatcata tttttcaacc aaacggatgg tggagaaaaa cgattttgca
     7741 ttacacaaaa tatcgattta ttgtgaatag cagcatttgc aataatactg tcgcaatcgg
     7801 caacagttaa tgccatgggt ttttcaatta ccacatgttt tccggcaagt aaagctaaat
     7861 tagcatgtgt agcatgtaag ccatttggtg tgcaaatatg cacaacatca atattttttt
     7921 cttcgctgag taattgttga atgtcactat aaaatttacc cggaaatttg tcaatgttta
     7981 attcttcctt aggttgtata tcacacaaag caactaattg agtgcggttg ttgcgcaaaa
     8041 tcatttctgc atgtcgcttc ccaatatgac cacatccaat tacagcacaa tttattttgt
     8101 tttcaatcac gtgttatcca attttactta cacgattatt tattaattga tacgaagcgc
     8161 ctgaaattgc acaagtcgct tttccatttt catcaaaaat taatttcgct ccatgttcac
     8221 tcatccatcc ctgatgtttt gcaggattac cggtaactaa agtgtagtcg tctatacttt
     8281 ttgtaacaac agcccccgcg ccgataaatg cataagcacc aatcgttatt ccacatacaa
     8341 tagttgcatt agcaccaatg gttgcccctt tttttactaa agtagatttg aattcatttt
     8401 tgcgctcaat ggcacttctg ggattaatca cattggtaaa taccatactt ggccctaaaa
     8461 aaacatcatc ttcacaaaca actcctgaat aattagatac gtcgtaatac aatttcacat
     8521 tg
//
LOCUS       DAORKW010000095         8490 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_224_length_8490_cov_5.394428, whole genome shotgun sequence.
ACCESSION   DAORKW010000095 DAORKW010000000
VERSION     DAORKW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224_length_8490_cov_5.394428"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLB46_05555"
     CDS             <1..340
                     /locus_tag="PLB46_05555"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRF76014.1"
                     /translation="QHKMPDSYGHAKDFFNPVIADGQLNTQRNLLQFTNGSKSQPKVN
                     DILVLDKSRTNLYGHVAIISAVEGGYIEIIQQNAGKYATSRDIMELVQEEGKWVIKND
                     RVLGWLRIKN"
     gene            393..1208
                     /locus_tag="PLB46_05560"
     CDS             393..1208
                     /locus_tag="PLB46_05560"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF76015.1"
                     /translation="MGSTNLFQYINSTFHYTIYGDGAEVLIAFHGFSESGRSFASLKL
                     ALSARYTIYAFDLPFHGATKWNEPRPFTTGDLEKIIIQFTQQHNFEKFAVLGFSMGGK
                     CALHLAQTMPQKITALWLLASDGIKTNKIYNVAVYPRWGRSLFKTTIQHPAWLFAVVN
                     IANKLRLLSPWLKKFTYNHMETKEKRERLYHTWISMAAFNPDINLVKKQINQHQIPVL
                     LFFGKRDEVIPVAVGEFFAEGLTSCLLTRLERGHYFIDEKINPSLTAALQWLK"
     gene            1276..2412
                     /locus_tag="PLB46_05565"
     CDS             1276..2412
                     /locus_tag="PLB46_05565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF76016.1"
                     /translation="MLLLNILVFTLIGGYVLLILYFLAGWLRLKEFEPGQKMPTTKVT
                     VVVPVRNESHNILNLLNNLKEQRYNKNLFEVIVVDDHSTDNTLQLIRNFQMPNLRVIA
                     LSDDPELALVNRVNKKIGIEKAVREATGELIMTTDADCHPGSNWIFTTVAYYEQHKPV
                     MIAGMVSYFHDTSFLGKFQTLDFLSLVGIAAASIQNGFYNLCNGANLAYTKSAFIEVD
                     GFRQIDHIPSGDDMMLMHKMAKKYGDKIAYLKNKDAIVYTSTEKDFVAFWNQRVRWTS
                     KSTHYEDKRITAILAFVYLMNLLIVLNLAIGLFFPPMLKLAMWMFLAKLCIDTLFTYA
                     VTKFFRRENLLWLFLPVQAVHVLYIILIAPAGVFGKYNWKGRRV"
     gene            2435..3817
                     /locus_tag="PLB46_05570"
     CDS             2435..3817
                     /locus_tag="PLB46_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF76017.1"
                     /translation="MRKQIIGIIIFMLVLAGIIAWFQHAASGVRFFVALINSIKVIAI
                     VGLVLFLIYYPFILLIRYIWKKRSAKLDLQHNQQAKAINRFIKNGNALIGSITIFYAI
                     LLALFAHLIAPDITPYANDQINELPFKEPGYTTMILQQPLELKPEKTGFIQWLLNGKK
                     VDYRQIPITDYTVVGDSLIVERYIGDGIAGRELHFLLADITGEKLTPEENRKIIEADW
                     IHQRKFILGTDDLGRDYLSRILLGIRVSLSVGVVAVLIALFIGIPLGALAGFYKQYPP
                     FIQLKKRKKLFFPVDGAIMWLINIVWAIPTLLLVFPIVFAFGQNFYTIFIAVGITMWV
                     DIARIVRGQVLQIREQEFIQAAKTFGFSDVRTIFRHILPNITGPVIVITAANFAYAIL
                     TEAGLSFLGIGVQPPAPSWGLMISKYKDNLITEPYLALIPGFAITILVLAFFMIGNGL
                     RDALHIKSRL"
     gene            3863..5113
                     /locus_tag="PLB46_05575"
     CDS             3863..5113
                     /locus_tag="PLB46_05575"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HRF76018.1"
                     /translation="MAIAGLNVMEMLRDQLKLKQLEIDSLLEITKAINSNKSASALFE
                     LFHRTLKDQIGVENILLYSNNGDDWEPVIGNKFISELNPGLINSLLKYNKLTDLSALN
                     GELPEYLKQYDILIPVFHKEKPLAYLLMSNPRVESYEPLEDKVKFAQTVTNIISVAIE
                     NKRLFKKEVEQQSFQRELELAAQVQSMLIPDTLPNDDKIQMAAIYLPHKNIGGDYYDF
                     IKLSEDEMFFCIADISGKGIAAAILMANFQAQIRELTKRNPVTIEEYMQELNKGVLMS
                     TKGEKFITLFLGKYNRKTRLLKYVNSGHNPPILVNTNGAKFLDKGTTILGMFNELPSV
                     KMGQVPIIEDTAIICFTDGLTDVVNEDNQVFPLDELIDLVKANYKGNIETLNKKIINE
                     IMRFKDEDGAVSDDLTVLSVKILK"
     gene            complement(5125..6591)
                     /locus_tag="PLB46_05580"
     CDS             complement(5125..6591)
                     /locus_tag="PLB46_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRF76019.1"
                     /translation="MLNLQAKPVELNIFWTATVLAIAAMFAGFMLEEPLFFLVPVGFL
                     FAYQLIINYKVIFFLLLLVTPGATEFYFTGGFSTTLPTEPIMIVLMLTFFFFLMMKRE
                     NLDKAFFTHPLAFVLYLHFIWMIFTSIFADEIVISLKYMLAKTWFIVAFFCVAGMVIK
                     NINHYKAAFWCLFVPTVLLTIYTLINHMHYQFRFSEVNKTMVPFFRNHVNYAVFLALM
                     LPLSIAATKWYERFTWQKMVLKLGVIIIMLGIYFAYTRSAWLSVMGAFFAYYLIKNNK
                     LIPAAFIAIVGVIIFVFYMMHDNKYLDYAPEYTKTIYHSDFSDHMESTISLEDVSSAE
                     RIYRWVAAVHMIEDKPVLGFGPGQFYFNYKEYTVNKFETYISRNEEQSTVHNYYLQIT
                     VEQGFIGITIWVILLLSILYLGQRLYNKYKDSEYKAMAMAITLSIITIIINISLSDLI
                     EADKIGTCFFMFIAILINLDVHYKRNQAAVETSKEVNL"
     gene            complement(6584..7483)
                     /locus_tag="PLB46_05585"
     CDS             complement(6584..7483)
                     /locus_tag="PLB46_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRF76020.1"
                     /translation="MEQSNDLVRLIQIIYKWRKSLIAVTVIVAIAACIFTWFFMPNYY
                     KSTVNFYPSNPIMTDRQVLFSQSSGEIEIEYFGSASDVDRILTIANTSGIVDYIINKY
                     KLMEHYDYDSTKEMARYNTKKEFLSNYSTIETEYGAIEISVWDQDKQLAADMVTHIVE
                     TIDDHNKAMLLRDKKLVVETFKKQVADKEALVTKLTDSIAVMKKSGAPSEVLLVLDSK
                     LEGAVEDLNNNKKILEQNQTSVNTDFSTVHITEEAYPAIRKDKPVRSLIVIGVTLGAL
                     FFMVILAVLTENYRKIKSKLKDA"
     gene            complement(7527..8441)
                     /locus_tag="PLB46_05590"
     CDS             complement(7527..8441)
                     /locus_tag="PLB46_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRF76021.1"
                     /translation="MGYVIAIIVATLVLSAKKLEIRFTVKTTLIKDIFRQSYPYALLG
                     FLMTAYYRIDGVLLERMLENGAYEAGVYASAFRLLDALAIAGFLIAGVLMPMFARMLE
                     EKQSIQPLLEIGFKVMLIISVCVGVGAIFYRNEIMELLYLSGDAYSGSIFGWLMVSFI
                     CISLTYIYGSLLTAGGKIALLNKISLAGFAINLILNMILIPKYLALGSAIATVAAQLL
                     VLIAHVMASEKTFHLTKSLQQWLKSGLFLALVTGINLLFAQLPINWVYALVMSGFVSL
                     LAVFPSTILTVSEVRQFLRTVSGEVSKK"
BASE COUNT         2790 a         1497 c         1587 g         2616 t
ORIGIN      
        1 ccaacacaaa atgcctgata gttatggcca tgcaaaagat ttttttaatc ctgttattgc
       61 tgatggacaa ttaaatacac aacgcaattt actccaattt acaaacggca gtaaatcaca
      121 accaaaagta aatgatattt tggtgttaga taaaagcagg acaaatctct acggacatgt
      181 tgcaattatt tcggcggtag aaggagggta tattgaaatt attcagcaaa atgccggaaa
      241 atatgctact tcacgcgaca ttatggaact ggttcaggaa gaaggcaaat gggtgataaa
      301 aaacgaccgt gttttaggat ggttgcgaat taaaaactga atatcaaaca gcgttttaat
      361 agatttattt aattttgact ggtattcaat aaatgggtag cacaaatctt tttcaatata
      421 taaattcaac ttttcactat accatttatg gtgatggagc ggaggtgtta attgcattcc
      481 atgggtttag tgaaagtggt agaagtttcg cctcgcttaa attggcattg tcggcacgtt
      541 acacgattta cgcatttgac ttaccattcc atggcgcaac aaaatggaat gagccaaggc
      601 cttttacaac cggcgatttg gaaaaaataa ttattcaatt tacacaacaa cataatttcg
      661 agaaattcgc ggtattagga tttagtatgg gtggaaaatg tgcattacat ctcgcccaaa
      721 caatgccgca aaaaataact gctttatggc tactcgccag cgatggcatc aaaacaaata
      781 aaatatataa tgttgctgtt tatccgagat ggggtagaag tttatttaaa acaactattc
      841 aacatccagc ctggttattt gcagtagtta atattgccaa caaattgcga ttgttaagcc
      901 cgtggttaaa aaaatttacc tacaatcaca tggagacaaa agaaaaacgc gaacgattat
      961 atcatacatg gattagtatg gcagcgttta atcctgatat taatttggta aaaaaacaaa
     1021 tcaatcaaca tcaaattccg gtattattat tttttggcaa acgcgatgaa gtgataccgg
     1081 ttgcagttgg tgaatttttt gccgaaggcc taacatcttg tttgctcact cgcctcgaac
     1141 gcgggcatta ttttatcgat gaaaagataa accccagcct tactgctgcc ttacagtggt
     1201 tgaaataata tttttacagt tatttaatgg cagtaaatgc cattttcagg cttgagatac
     1261 ctaattttgc agcgcatgct cctgctcaac atacttgttt ttacgctcat tggtggctat
     1321 gtgttgctca tcctgtattt tttggcaggc tggttgcgac taaaagaatt tgagccggga
     1381 caaaaaatgc ctactaccaa agttactgtt gtagtgcccg ttcgaaacga atcgcacaat
     1441 attctcaatc tgctgaataa tttaaaggag cagcgttaca ataaaaattt attcgaagta
     1501 attgtcgtag atgatcattc tacagataat accctacagt taatccgaaa ttttcaaatg
     1561 cctaatttac gtgtcatcgc tttatccgac gacccggaat tagcattggt aaacagagta
     1621 aataaaaaaa taggcatcga aaaagcagtc cgtgaagcaa caggcgagtt aatcatgacc
     1681 accgatgccg attgccaccc gggcagcaat tggattttta ccacagtagc atattatgag
     1741 cagcataaac cggttatgat tgccggcatg gtaagctatt ttcatgacac atcgtttttg
     1801 ggaaaatttc aaacacttga ttttttaagt ttagtgggca ttgccgctgc aagtatccaa
     1861 aatggatttt ataatttgtg taatggtgca aatttggcat atactaaatc ggcatttatt
     1921 gaagtggatg gctttagaca aattgaccat attccaagtg gcgatgatat gatgctgatg
     1981 cataaaatgg caaaaaaata tggggataaa attgcctatc tgaaaaataa agatgccatt
     2041 gtatatactt ctacagaaaa ggattttgtt gcattttgga atcagcgtgt tcgttggaca
     2101 agcaagtcga cacattatga ggacaaaagg ataactgcaa tattggcttt tgtttatctg
     2161 atgaatttgc tcattgtgct taacctcgct ataggattat ttttccctcc aatgctcaaa
     2221 ctggcgatgt ggatgttttt ggccaaattg tgtatagata ccttattcac ttatgctgtt
     2281 actaaatttt tcagaaggga aaatttactt tggttatttt tacccgtgca ggcagtgcat
     2341 gtgttgtaca ttattcttat agctcctgca ggtgtattcg gaaaatacaa ctggaaagga
     2401 agaagggttt aattgagtgc ctgcaaatta aatcatgcgt aaacaaatta ttggcattat
     2461 tatttttatg ttggtgctgg ccggtattat agcctggttt cagcatgctg cctccggtgt
     2521 acggtttttt gtagcattga ttaattccat caaagtaatc gcaattgttg gcctggtatt
     2581 atttctgatt tattatcctt tcatactatt gatacgatac atttggaaaa agcgaagtgc
     2641 caaacttgat ttacaacaca atcaacaagc aaaagcaatt aaccgtttta ttaaaaatgg
     2701 caacgcacta atcggttcca taactatttt ttacgccatt ttattagcct tatttgcaca
     2761 tttaattgcg ccggatatta ccccttatgc caacgaccag ataaatgaat taccatttaa
     2821 ggagcccggt tataccacaa tgatattgca gcaaccactg gaattgaaac ccgaaaaaac
     2881 cggatttatt caatggttac taaatggtaa aaaagtggat taccgacaaa tcccaatcac
     2941 agattatacg gttgttggcg actcgttaat agtagaacgt tatattggtg atggcattgc
     3001 aggaagagaa ctgcattttt tattagcaga tataacaggt gaaaaattaa cgcctgagga
     3061 aaatcggaaa attatagaag cagattggat acatcagcga aaatttattt taggaaccga
     3121 cgatttggga cgagattatc tcagtcgtat tttattagga atcagagttt cattatctgt
     3181 tggagtggtt gctgttttaa ttgccttatt tatcggaatt ccacttggag ctttagccgg
     3241 tttttacaaa caatatccgc catttattca attaaaaaaa cgcaagaaat tatttttccc
     3301 tgttgatggt gctatcatgt ggctgattaa tattgtttgg gcaattccta ctttattact
     3361 ggtatttcca attgtgtttg catttgggca aaatttttac accatattta ttgctgtcgg
     3421 catcaccatg tgggtcgata ttgccagaat agtgagagga caggtattac aaattcggga
     3481 gcaggaattt attcaggcag ctaaaacgtt tggctttagc gatgtaagga caattttccg
     3541 tcatattttg ccaaatatca caggaccggt aattgtaata acggctgcaa attttgcgta
     3601 tgctatttta acagaagcag gattgagttt tttgggcatt ggcgttcaac ctcctgcccc
     3661 aagctggggt ttgatgatat caaagtataa agataatttg attaccgagc cctacctcgc
     3721 tttgattccg ggctttgcaa tcactatctt agtacttgca ttttttatga taggcaatgg
     3781 attacgtgat gcgctgcata ttaagagtcg attgtaaaag aaaatgcaca aaaagattta
     3841 tttttaaagt gaaatacaag taatggcaat cgcaggatta aatgtaatgg aaatgttgcg
     3901 cgaccagttg aaactcaaac aactggagat agattcctta ttggaaataa ccaaagccat
     3961 aaattctaat aaatcagcct ccgcattatt cgaattattt caccgcacgt taaaagacca
     4021 aattggtgta gaaaatattt tattgtatag taacaatggt gatgattggg aaccggtaat
     4081 cggcaataag tttatttctg aactaaatcc ggggttaatt aattctttat tgaaatataa
     4141 taaactcacc gacctgagtg cactcaatgg ggaattgcct gagtacttaa aacaatatga
     4201 tattttaatt ccggtattcc ataaagaaaa accacttgct tatttactta tgagtaatcc
     4261 gcgagttgaa agttatgagc cgctggagga caaagtaaaa tttgcacaaa cggtaacgaa
     4321 tattatcagc gtagcaattg aaaataaacg tttgtttaaa aaggaagtgg agcaacaatc
     4381 gtttcagcgt gaacttgaat tagctgcgca agtgcaaagt atgcttattc ctgacacctt
     4441 gcccaacgat gataaaattc aaatggcggc catttatttg ccacataaaa atattggcgg
     4501 cgattattac gactttatca aattgagtga agatgaaatg tttttttgta ttgccgatat
     4561 ttctggaaaa ggaattgcag cagcaatatt aatggctaac ttccaggcgc aaattcgcga
     4621 gctgacaaaa agaaatcctg ttactattga agaatatatg caggaactta ataaaggtgt
     4681 gttgatgagt accaaagggg aaaaatttat caccttattt ttaggtaaat acaatcgcaa
     4741 aacacgtttg ttgaaatatg tcaactccgg gcataatcct cccattttag tcaacaccaa
     4801 tggcgcaaaa tttttagata agggcactac tattttgggt atgtttaatg agttgccatc
     4861 agttaaaatg ggacaggtgc caataattga agatactgcc atcatctgtt tcaccgacgg
     4921 acttaccgat gtggtgaatg aagacaatca ggtgtttcca ttagatgaat taatcgattt
     4981 ggtgaaggcc aactacaaag gcaatatcga aacgctcaac aaaaaaatca ttaacgaaat
     5041 tatgcgcttt aaagatgaag atggcgctgt tagcgatgat ctcaccgtat tgtctgtaaa
     5101 aattttgaag taattacctt ataattacaa attcacttcc ttgcttgttt caactgcggc
     5161 ttgatttctt ttataatgca catctaaatt tatcagaatt gcaataaaca taaagaaaca
     5221 agttccaatt ttatcagcct caattaagtc ggaaagtgaa atatttataa ttatggtgat
     5281 aatggaaagc gttatggcca ttgccattgc cttgtattcc gaatctttgt atttattata
     5341 taatcgctga cctaaatata aaatagagag tagtaatata acccagatag taataccgat
     5401 aaaaccttgc tcaacagtta tctgaagata ataattatgc actgttgact gttcctcatt
     5461 tctactaata taggtttcga atttattgac cgtatattct ttataattaa aataaaactg
     5521 tcccggtcca aaacctaata caggtttgtc ttcaatcatg tgtactgctg ctacccaacg
     5581 ataaattctt tccgcactgg aaacatcttc taaactgatg gtagattcca tgtgatcgga
     5641 aaaatcactg tgataaatag ttttagtgta ttcaggtgca taatccaaat acttattatc
     5701 atgcatcata taaaaaacaa aaataataac cccgacaatg gcaataaaag cagccggaat
     5761 taatttattg tttttaatta agtagtaagc aaaaaatgca cccataactg ataaccaggc
     5821 agaacgcgtg taggcgaagt aaatgcctaa cataataata attacaccca atttcaatac
     5881 cattttttgc caggtaaaac gttcatacca tttagtggca gcaatactaa gtggcaacat
     5941 gagcgctaaa aaaacggcat agttaacatg atttctaaaa aacggcacca tggttttatt
     6001 tacttcgcta aacctaaatt ggtagtgcat gtggttaatt agtgtgtaaa tagtaagcaa
     6061 caccgtaggc acaaataaac accagaatgc agctttgtaa tggttaatgt ttttaattac
     6121 cattccggcc acacaaaaaa atgcaactat gaaccatgtt ttggctaaca tgtattttaa
     6181 tgaaataacg atttcatcgg caaatatgga agtgaaaatc atccatataa aatgcaggta
     6241 taatacaaag gcaagcggat gggtaaaaaa tgctttgtcc agattttctc ttttcatcat
     6301 taaaaaaaag aaaaaggtaa gcatgagcac tatcataatt ggctcggttg gcagggtggt
     6361 gctgaaacca ccggtgaaat aaaattcggt tgcaccgggt gttaccaaca ataataaaaa
     6421 gaaaattact ttataattga taatcagctg gtaagcaaat agaaaaccaa caggaactaa
     6481 aaaaaataaa ggttcctcca gcatgaatcc tgcaaacata gcggctatgg caagaacagt
     6541 tgctgtccaa aaaatattta attcaacagg tttcgcctgc agattaagca tctttaagtt
     6601 tggattttat ttttctgtaa ttttcagtga gtactgctaa aattaccatg aagaataatg
     6661 cacctaaggt tacaccgatt acaattaacg aacgcaccgg tttgtctttt ctaattgcag
     6721 gataagcctc ttcggtgata tgcacggttg aaaaatcggt gtttactgag gtttgatttt
     6781 gttctaatat ttttttgttg ttgtttaaat cttcaactgc accttccaat ttgctatcta
     6841 aaaccaatag cacttcactt ggagcgcctg attttttcat tacagcaata ctgtctgtta
     6901 atttagttac taatgcttct ttgtctgcaa cttgtttttt aaatgtttca acaactaatt
     6961 ttttgtcgcg taacaacatg gctttattgt gatcatcaat tgtttcaaca atatgtgtta
     7021 ccatatctgc agctaattgt ttatcctggt cccaaacgga aatttcaatg gcgccatatt
     7081 ctgtttcaat agtgctgtaa ttacttaaaa attctttttt ggtattataa cgtgccattt
     7141 ctttggtgga atcataatca taatgttcca tcaatttgta tttatttata atatagtcaa
     7201 caattccact ggtattggca atggttaaaa tgcggtctac atcactggca ctaccgaaat
     7261 attcaatttc aatttcacct gacgactggc taaaaagcac ttgtctgtcg gtcatgatcg
     7321 gattagaagg gtaaaagtta acggtagatt tgtaataatt tggcataaaa aaccaagtga
     7381 aaatacaggc cgcaatagct acgataacgg ttactgcaat cagcgattta cgccatttat
     7441 aaataatttg tatcaaccta accaggtcgt tggattgttc catgcttaaa atttagagtg
     7501 caaaggtaga aaaactagct tattgttcac tttttggata cttcaccgct cacagtgcgc
     7561 aaaaactgtc gcacttccga tacagttaaa attgtagaag gaaatacagc cagtaaactc
     7621 acaaatccgc tcatcactaa ggcatacacc caattaatag gcaattgtgc aaataataga
     7681 ttgataccag taaccaaggc taaaaacaaa ccacttttta accattgttg taagcttttt
     7741 gtaaggtgaa acgttttttc tgaagccatt acatgcgcaa ttaacaccaa taattgggca
     7801 gctactgttg ctattgcact gcctaatgcc agatatttag ggatgagtat catgttgaga
     7861 atcaggttga tagcaaatcc ggccaatgaa attttgttta agagcgcaat tttccctcca
     7921 gcggtaagta aactgccata tatataggta aggcttatac aaatgaaact caccatcagc
     7981 cagccaaaaa tggagccgga gtaggcatcg ccggaaaggt ataagagctc cataatctca
     8041 ttccggtaaa aaatggcccc aacaccaacg caaacgctta taatgagcat caccttaaaa
     8101 ccaatttcca gcaacggttg tatcgattgt ttttcttcca gcattcgagc aaacatcggc
     8161 atcaatacac cggcaatcag gaatcccgca atggccaaag cgtccagcag cctaaacgct
     8221 gaggcatata cccccgcttc ataagcgcca ttttcgagca ttcgctccag taaaacgccg
     8281 tcaattcggt aataagccgt catcaaaaat cccaatagtg cataaggata actttgacgg
     8341 aaaatgtctt ttatgagggt ggttttaacc gtaaaacgta tttccagttt tttggctgaa
     8401 agtactagtg tggcaacaat tattgcaata acgtaaccaa taatctgact ttaaatgaat
     8461 aaggttgcac ttatcggtga tttaattacc
//
LOCUS       DAORKW010000096         8441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_228_length_8441_cov_4.982352, whole genome shotgun sequence.
ACCESSION   DAORKW010000096 DAORKW010000000
VERSION     DAORKW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228_length_8441_cov_4.982352"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2142)
                     /locus_tag="PLB46_05595"
     CDS             complement(<1..2142)
                     /locus_tag="PLB46_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76022.1"
                     /translation="MKHIQLFLVLVFTQLALAAQKYEQAYAAGNFEKAIKYAEKAIEA
                     DGKNLDAYLIKAMANYHLGSDSATREENSFGIESAFSTLKLIIRKDKSGAFVEAHQAE
                     ADTILFAIYQVANTALEKGKTDKAERIINDLIEIEPKPAYYYLVGKMWLDQGNEFKAI
                     DMFNDAAAKIYLDNKAGKKPEPYLFEIFQVLAENIALKGDYTSAYTIFNRALLLFDDK
                     EIDEVYYQFLFPIAEYNYFSYDTLRQKSFIFNLDTAAQLTQNSQQFNELKWKMLMQFY
                     TTNVADNYYEGASFLSEYICAESKKDFNQFFYHQIIDATQVSVTIDGTVPLDGRQYMF
                     TWLRLQDCLYERNNLTAMTRVMDSLLQNQHRVEASKWLYNLKIQKVDAKKITEYEQAL
                     YQLIKNADTAQFAFMDLYDLTFYFPQNKNFKSLQEKGAYAEIVKLINNNQFSAAGKLL
                     RKQIRNTPNDKTINDLYKRWVIADYQTNYLGSAYYIDNDEWGGSVESCEPGKLTAEMQ
                     KKFLQRLNYARRVAGVPDNCELRESWNAYCQSAALMMTAEGDLSHFPGKDWKCYSPEG
                     AKGAGNSNLSYGYSAVSALMGQLDDSGANNYFVGHRRWILNPSRKVFGHGSTGYAMAL
                     WALGGENSDYDAAIVKEFETQYVCWPPEFYFPAVLNTARWSFSLRGADFDSVTVEMYW
                     GNKKIDVSIQEWTPGYGMSTIVWEPQTTYYDY"
     gene            2208..2402
                     /locus_tag="PLB46_05600"
     CDS             2208..2402
                     /locus_tag="PLB46_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76023.1"
                     /translation="MAFSDSAFGAVVTCKCPRCRKADMFTNKNPYVFNEMAKMPKHCP
                     NCGLKLEPETGFYYGSMYLS"
     gene            complement(2585..3229)
                     /locus_tag="PLB46_05605"
     CDS             complement(2585..3229)
                     /locus_tag="PLB46_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76024.1"
                     /translation="MMKIFAPVFVALLLVVSCKNYIGIPDNFDYGKIENNVYTNEFFG
                     MRMPLNDGWTSLEKDFVDSLRAEGLKDMVGDNKELEKTISAADIKTANLISILHADTT
                     VFQLYNPNISMIAENLKLALQVKNGKDYLEAAKSFMEGSGVDFQFVGDIRAYKIGGKE
                     FYTMEVINTVQGVPIHQDFYAAVMNGFGLAIVGSWMDEEQRAIIRSELETITFD"
     gene            complement(3258..4727)
                     /gene="dacB"
                     /locus_tag="PLB46_05610"
     CDS             complement(3258..4727)
                     /gene="dacB"
                     /locus_tag="PLB46_05610"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HRF76025.1"
                     /translation="MKKLLTILFIGITAFAQSQTKIDKIGINKLNGGLKALAADEDLK
                     HANWSFCITDPQTGKVLAAHESEKSLVPASSQKVITTIAGLNLVGADYTYKTYIEYDG
                     TIGADSVLKGNLYVRGTGDPTLGSERLANMLRYDSLMVTWAKLIKEKGINQITGKIIA
                     DASVFEDYATPGSWNWDDIGQYYGAGSYGLNIFENTFTLYYSSTSSTCRVDSMFPKIE
                     DLTVWNDVKVGGGGDEAYIYGAPDNYYRYVTGTIPANRKAYTVDGSMPDPPLYLALEL
                     KKALINNGIKVDQQATTVYAMKREKLMVNNERKNLYTHVSAPLSDVIYHTNQKSNNLY
                     AETILKTIGKKQLSDGSRESGTKAVAAYYKNKGIDLAGMNIEDGSGLSRMNVFTTKQM
                     CSILNMEMKEPTFEQFKKSLPIAGKSGTLITVADNTVAAGKVFAKSGSMYKVRSYSGY
                     VQTKSGELLSFCVIVNNYTCGSVEIKSKLEKLMVLMAGL"
     gene            complement(4769..5503)
                     /gene="dapB"
                     /locus_tag="PLB46_05615"
     CDS             complement(4769..5503)
                     /gene="dapB"
                     /locus_tag="PLB46_05615"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRF76026.1"
                     /translation="MNIALIGYGKMGKAIEAIIRKHNAMHSNHMHVVAQFSKTNHPLI
                     SAAQLKGVDVAIEFTTPLTAVEHMKLCFDAGVPVVVGSTGWYHQLDEVTTYCAEKKGS
                     LIYAANFSIGVNLFFKINAMLASLMQSQPQYQLSISETHHTQKLDAPSGTAIKTAQVI
                     LENWSHKNTWKLAPTTDENAIPITAHRVEHVPGTHEVIYRSAVDEIQLTHTAHSRDGF
                     AEGALIAAKWLVNKKGVFTMQDVLAI"
     gene            complement(5500..6135)
                     /locus_tag="PLB46_05620"
     CDS             complement(5500..6135)
                     /locus_tag="PLB46_05620"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HRF76027.1"
                     /translation="MKYVCIVIGFFCATLAQAQTDTIAVTDTIITAAIDTSAVEFDSL
                     HTPFYKNLWSHNPHSVGRAALYSAIIPGLGQAYNGKYWKIPIVYGAIGTAGYFIYYWY
                     DFYDELRTAYKYRTDDDPLTVDVIYDYIADDAYLYDRVSLAKQYTDLMVVLTAATYVL
                     NIVDAVVDAHLYDFNVSDDLTMQIHPYFNPNLAAANTFQPTYGIGLKFSLK"
     gene            complement(6144..7058)
                     /locus_tag="PLB46_05625"
     CDS             complement(6144..7058)
                     /locus_tag="PLB46_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRF76028.1"
                     /translation="MSQKKPDLGKGIRALLQSMESETSVEINEKKSAQQETQNNDAVN
                     HIALDTIEVNPFQPRADFDETALKELADSIKIHGVVQPITVRKVGTNKYQLISGERRT
                     RASKLAGLKEIPAYVRAANDQEMLEIALIENIQREDLNAIEVAIHFRRLLDECNLTHE
                     TLSDRVGKDRSTVTNFLRLLKLPPEVQRGLKEKKISMGHARAILALEDPIAQIDIYKE
                     TITRNLSVRDVELLARDYRGKKQSSGSNSIKKSTSLSFELKKVQDSLASHLATRVQIK
                     KDKQGKGEITISFFSDDDLMRIKDIIED"
     gene            complement(7149..7961)
                     /locus_tag="PLB46_05630"
     CDS             complement(7149..7961)
                     /locus_tag="PLB46_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF76029.1"
                     /translation="MGKIIAIANQKGGVGKTTTAINLAASLAVLEFKTLLVDADPQAN
                     STSGVGFDPKENRVNIYDCLMNDKLAKDAIVETETPNLHLLPSHLDLVGAEIELINHP
                     NRERIMKTILEEVKNNYDYIFIDCSPSLGLITVNALTAADSVLVPVQCEYFALEGLGK
                     LLNTIKIVQNRLNNPLQIEGILLTMYDARLRLSNQIVTEVKRHFSDMVFDVIVHRNTK
                     LGEAPSFGKPVIMYDADSTGAVNYLNLAREILQKNGGINPQHTNELLKVNQN"
     gene            complement(8007..>8441)
                     /locus_tag="PLB46_05635"
     CDS             complement(8007..>8441)
                     /locus_tag="PLB46_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF76030.1"
                     /translation="TNAHRMNSGGVWNFDFGSGKMVNAVHSSSFEDGSYGGQAAGFYF
                     ETDEANFYYAGDTALHYDMKLLGKHFDIDIAFLPIGSNFTMGIDDAMIASKYIKCDKV
                     IGMHYDTFGFIKIDHDEAKKKFMSQDKELILMDINTSKTFTF"
BASE COUNT         2490 a         1690 c         1405 g         2856 t
ORIGIN      
        1 gtaatcataa taagtagttt gaggttccca tactatagtg ctcatgccgt atccaggcgt
       61 ccattcctgt atactcacat caattttttt attgccccaa tacatttcaa ctgtaacact
      121 gtcgaaatcg gcgccacgta atgaaaatga ccaacgcgca gtatttaata cagcaggaaa
      181 ataaaattcc ggaggccaac aaacatattg tgtttcaaat tcttttacaa ttgccgcatc
      241 ataatctgaa ttttcaccac ctagtgccca taacgccatt gcataaccgg ttgacccatg
      301 tccaaatact ttgcgtgaag gattcaatat ccatcttcta tgaccaacaa aataattgtt
      361 ggcaccacta tcatctaatt gtcccattaa cgcactcact gcactataac cgtacgacaa
      421 attagaatta cctgccccct ttgctccttc aggcgaataa catttccagt cctttccggg
      481 aaaatgcgac aaatcgcctt cagctgtcat cattaatgct gcagactgac aataggcatt
      541 ccacgattca cgtaattcgc aattgtcagg cacacctgca acacgtcttg cataatttaa
      601 tcgctgcaaa aatttctttt gcatttcagc agttaatttt ccgggttcac aagattcaac
      661 tgagcctccc cattcatcgt tgtcgatata gtatgcactt cctaaataat ttgtttgata
      721 atcggcaatt acccaacgct tatataaatc gtttatggtt ttatcgtttg gtgtgttacg
      781 aatttgttta cgcaataatt tccctgcagc actaaactga ttgttattaa ttaatttcac
      841 aatttccgca taagcacctt tttcctgcaa tgatttaaaa tttttgtttt gaggaaaata
      901 aaatgtcaaa tcatataaat ccataaatgc aaattgagcg gtatctgcat tcttaatcag
      961 ttgatataac gcttgttcgt actccgtaat tttttttgcg tctaccttct gaatctttaa
     1021 attatacaac cattttgatg cttcaactcg atgctgattt tgcaacaaac tgtccattac
     1081 acgggtcatg gctgttaagt tgttacgctc gtataaacaa tcctgcaacc ttaaccaggt
     1141 aaacatatat tgccttccat cgagtggaac agtgccgtca atagttacgg aaacctgcgt
     1201 agcatctatt atttggtgat aaaaaaattg attgaaatct tttttcgatt cagcacatat
     1261 atattcgctc aaaaaacttg caccttcgta ataattgtcc gcaacatttg tagtataaaa
     1321 ctgcattaac attttccatt tcagctcatt aaattgctga gaattttgcg ttaattgtgc
     1381 tgctgtatct aaattaaaaa taaacgattt ttgtcgcaat gtatcgtaag agaaataatt
     1441 atattcagca attggaaata aaaattggta atacacctca tcaatttctt tatcatcgaa
     1501 taacaataat gcgcggttga aaatagtata agcactggta taatcaccct tcaaagcgat
     1561 attttcagcc aatacttgaa atatttcgaa taaatagggt tcaggttttt taccggcttt
     1621 attatctaaa taaattttgg cagccgcatc attaaacata tcaatggctt taaattcgtt
     1681 gccctgatcc agccacattt taccgaccaa ataataatat gcaggtttgg gttcaatctc
     1741 aattaagtcg ttgataattc tttccgcctt atctgttttc cctttttcca aagctgtatt
     1801 ggcaacctgg tatatcgcaa ataaaatggt gtcagcttca gcctgatgtg cttcaacaaa
     1861 tgcacctgat ttatcttttc ggataatgag ttttagggta gaaaaagcgg actcaatacc
     1921 aaagctattc tcctctctgg ttgcagaatc ggaacctaaa tgataattag ccatggcttt
     1981 tatcaaataa gcatccagat ttttaccatc agcctctatt gctttttcag cgtatttgat
     2041 agctttttca aaatttccgg cagcataagc ctgctcatac ttttgtgcag caagcgccag
     2101 ttgagtaaaa actaaaacca ggaataattg tatgtgtttc aaactgttgt ataaacgaag
     2161 atgtaaaaat atttagtatt aaataaataa taagcgtaat tttgtacatg gctttttctg
     2221 attctgcttt tggggcagtt gttacctgca aatgcccacg ttgtcgcaaa gccgacatgt
     2281 ttactaacaa aaatccttat gtcttcaatg aaatggctaa aatgcctaaa cattgtccta
     2341 attgtggttt aaaactggag cctgaaaccg gcttctacta tggctctatg tatttgagtt
     2401 aagcaattgg tgttttttcc agcctgatat tttttgccat tttaaatttt ggctttggta
     2461 tacagactac ttatgcattt ataattgttg ccggattgtg gcttttgggt tctccgtatt
     2521 tgtttcgatt ttcccgtgca ctatggttga gtttatacgt aaaccggcat cgcgatattt
     2581 aatatcagtc gaaagttatc gtttctaatt cggagcgaat gatagctctt tgttcttcat
     2641 ccatccaact gccgacaatt gcgagtccaa atccgttcat tacagctgca taaaaatctt
     2701 ggtgaatagg cacaccctga acagtattta tcacttccat tgtataaaat tccttgccgc
     2761 caattttata tgcacgaata tcgccaacaa actgaaaatc aacaccactg ccttccataa
     2821 acgatttagc agcttcgaga taatctttac catttttcac ctgtaatgct aatttcaggt
     2881 tttcggcaat cattgaaata ttgggattat aaagttgaaa tacggtagtg tcagcatgca
     2941 aaatggaaat cagatttgct gttttgatat ccgctgcact aatcgttttt tccagctctt
     3001 tgttatcgcc taccatatct ttcaaaccct ctgcacgcaa gctgtccaca aaatcttttt
     3061 ccaaagaagt ccacccgtca tttaatggca ttctcatacc aaaaaattcg ttggtataaa
     3121 cgttattttc aattttaccg taatcgaaat tatcgggaat accaatataa tttttacagg
     3181 aaactaccaa caacaaggct acaaaaacgg gcgcgaatat cttcatcaat gtttaatttt
     3241 tgataaatat acaaaaatta taagcctgcc atcaatacca ttaatttttc caatttactt
     3301 tttatttcaa ctgaaccgca agtgtagttg ttaacaatga cacaaaaact taataattcg
     3361 ccgctttttg tctgcacata tccggagtat gaccttactt tatacatgga gccacttttt
     3421 gcaaacactt tacctgcagc aactgtattg tctgcaaccg taattaaagt gcctgatttg
     3481 cctgcaatgg gcaaactctt tttaaattgt tcgaaagtag gctctttcat ttccatattc
     3541 aaaatggaac acatctgttt agttgtaaaa acattcatgc gcgataaacc actgccatct
     3601 tcaatattca taccggccaa atcgatacct ttatttttat aatatgcagc tactgctttt
     3661 gtacctgatt cacgactgcc gtcactcaac tgtttttttc caatagtttt taaaattgtt
     3721 tccgcataca gattattact tttctgattg gtgtgatata ttacatcact tagtggtgca
     3781 gatacatgtg tatataaatt ttttcgttca ttatttacca ttaatttttc acgtttcatg
     3841 gcgtaaacag tagtcgcttg ctggtctact ttaattccat tatttatcaa tgcttttttt
     3901 aattccaaag ccaaatacaa tggaggatcc ggcatgctgc cgtcaacagt ataagctttt
     3961 ctgttagcag gaattgttcc ggttacatat cgataataat tatccggtgc accataaata
     4021 tatgcctcat ctcccccgcc acccacttta acatcgttcc aaacggttaa gtcttcaatt
     4081 ttgggaaaca tactatctac ccgacaagta cttgatgtag aagaataata taaggtaaag
     4141 gtattttcaa aaatatttaa accatatgaa cctgctccgt aatattgacc aatatcgtcc
     4201 caattccaac tgccgggagt ggcataatct tcaaatacac tggcgtctgc aattatttta
     4261 cctgtaattt gattgatacc tttttctttg atgagttttg cccaggttac catcagtgaa
     4321 tcatatcgca acatattagc cagtcgttca ctgcccaatg ttggatcacc ggtaccgcgc
     4381 acatataaat taccttttaa taccgaatca gctccaattg ttccatcata ttcaatataa
     4441 gttttgtagg tgtaatcagc acccactaaa tttaatccgg ctatggtagt aattaccttt
     4501 tgactggaag ctggcactaa acttttttca gattcatgtg ccgctaaaac ttttcctgtt
     4561 tgcgggtctg taatgcaaaa actccagttt gcgtgtttta aatcttcatc tgcagctaat
     4621 gcttttaatc cgccatttaa tttattaatc ccaattttgt caattttggt ttgggattgc
     4681 gcaaatgcag taattccaat gaataatatc gtgaggagtt ttttcataac ctgaaatatc
     4741 gtaactaaac gtataaatga attaaaaatt atatcgccaa aacatcttgc atagtaaata
     4801 cacctttttt atttaccagc cattttgctg caataagagc gccttctgca aaaccatcac
     4861 gcgaatgcgc agtgtgggtt aattggattt catcaactgc agaacgatat attacttcgt
     4921 gtgtgccggg aacatgttca acgcgatggg cagttatcgg aatagcattt tcatctgttg
     4981 ttggcgctag tttccaggta tttttatgtg accagttttc aagaattacc tgtgctgttt
     5041 ttatagcagt gccacttggt gcatctaact tttgggtatg atgtgtttcg cttatggaca
     5101 attggtattg aggctgagat tgcattaggg atgctagcat cgcatttatt ttaaaaaata
     5161 aattaacacc tatactgaag tttgctgcat aaattaagga gccttttttt tctgcacaat
     5221 aggtagttac ttcatcaagt tgatgatacc agccggtaga acctaccaca acaggaacac
     5281 cagcatcgaa acacagtttc atatgctcaa ctgccgttaa aggagtggta aattcgatag
     5341 cgacatcaac accctttaat tgtgctgcag atattaatgg atgatttgtt tttgaaaatt
     5401 gggcgacaac atgcatatgg ttactatgca tagcattgtg tttgcgaata atggcctcta
     5461 tcgctttgcc cattttacca tatccaataa gtgctatatt cattttaaac tgaattttaa
     5521 acctattcca taagtgggtt gaaatgtatt tgcagcagct aaattagggt taaaataagg
     5581 gtgaatttgc atggttaaat cgtcgctcac attaaaatca tacaagtgtg catcaacaac
     5641 tgcatctaca atattcaata cataagttgc tgcagttaac accaccatta aatcggtata
     5701 ttgtttcgcc agactaaccc tgtcatataa ataagcgtca tcagcaatat aatcataaat
     5761 tacatcgaca gtcagcgggt catcatcagt tctgtattta tatgctgtgc gtaattcatc
     5821 gtaaaagtcg taccagtaat aaataaaata accggctgta ccaattgctc catatacaat
     5881 tggtattttc cagtatttgc cgttataagc ctgcccaagc cccggaatta ttgcactgta
     5941 taaagcagca cgaccaacac tatgcggatt atgcgaccat aaatttttat aaaaaggcgt
     6001 atgtaacgaa tcgaattcaa ctgctgaagt atcgattgca gcagtaatga tggtatctgt
     6061 tacggcaata gtatcggttt gagcctgcgc taaagtggca caaaaaaaac caattactat
     6121 gcatacgtat ttcattgcct caattaatcc tcaattatat ctttaatgcg cattaaatcg
     6181 tcatcagaaa aaaatgagat ggtaatttct cctttccctt gtttatcttt tttaatttgc
     6241 acgcgagtgg ctaaatgaga tgccagacta tcctgcactt ttttcaattc aaaagaaagc
     6301 gatgtagatt tttttattga atttgaacct gatgattgtt tttttccacg gtagtcgcgg
     6361 gctaataatt caacatcacg taccgataaa tttctggtga tagtttcctt ataaatatca
     6421 atttgtgcaa ttgggtcttc caaagctaaa atagcacgtg catggcccat tgatattttt
     6481 ttctctttta atccgcgttg tacttccggt ggtaatttta gtaaacgcaa aaaattggta
     6541 actgttgaac ggtcctttcc tactctgtcc gagagtgttt catgcgtaag attacattca
     6601 tccaataatc ttctgaaatg aatagctact tcaattgcat ttaaatcttc gcgttgaata
     6661 ttttcaatca gcgcaatttc gagcatttcc tggtcattgg ctgcgcgaac ataagcagga
     6721 atttctttta atccggcgag tttggaagcg cgggtgcggc gttcaccgga aatgagctga
     6781 tatttattcg tgccaacttt acgaacagta atcggctgaa caacaccatg aatttttatc
     6841 gaatcggcta attcctttaa tgcggtttcg tcgaaatcgg ctcgtggttg aaatggattt
     6901 acttcaatag tatctaatgc aatatggtta acggcatcgt tattttgcgt ttcctgttgc
     6961 gcagattttt tttcgttgat ttctactgaa gtttcagatt ccatgctttg aagcaatgct
     7021 ctgatacctt ttcccagatc cggttttttc tgactcatac acttatggat ttatgacctc
     7081 tattcaaaaa gtatacccta agcattgata tgccagggta taattgttat aagtaacgat
     7141 aaatttaatc aattctgatt aaccttcaat aattcattgg tatgctgagg attgataccg
     7201 ccgttttttt ggagaatttc tctggccaaa ttgaggtaat ttactgctcc ggtgctatct
     7261 gcatcgtaca taataacagg tttaccaaaa ctaggtgctt cgcctaattt agtattgcga
     7321 tgtacgatta catcaaatac catatcggag aaatggcgtt ttacttcagt tacaatttga
     7381 ttgctcaagc gcaaacgggc atcgtacatg gtaagtaaaa taccttctat ttgtaatgga
     7441 ttatttaaac gattttgtac tattttgatg gtatttaata atttaccaag accttcaagg
     7501 gcaaaatatt cgcactgcac aggaaccaat acagaatctg cagcagttaa cgcgttaacc
     7561 gtaattaaac ctaatgatgg tgaacaatca ataaagatat aatcgtaatt atttttcact
     7621 tcctccaaaa tcgttttcat aatgcgttcg cgatttgggt ggttgattaa ttcgatttca
     7681 gccccaacta aatcgaggtg agatggcaat aaatgcaagt ttggtgtttc cgtttcaaca
     7741 atagcatctt tagccaattt gtcgttcatc aaacaatcgt aaatatttac cctgttttcc
     7801 tttgggtcga aaccaacacc actcgttgag tttgcctgag gatcggcatc taccagtaag
     7861 gttttaaatt cgagcaccgc cagtgatgct gcaaggttga tggctgttgt tgtttttcct
     7921 actccacctt tctgatttgc aatcgcaata atttttccca tcgttttggt tgttatttat
     7981 aattgtttag ctgaattatg tttcaattaa aatgtaaatg ttttgctggt attaatatcc
     8041 attaatatga gttctttgtc ctgactcata aatttctttt tggcttcatc gtgatctatt
     8101 ttaataaatc caaacgtatc gtaatgcata cctattactt tatcgcattt aatatatttg
     8161 gaagctatca tagcatcgtc gatacccatg gtaaaattgc tgccaattgg taaaaaggcg
     8221 atatcgatat caaaatgttt gcccaacaat ttcatatcgt aatgtaaagc cgtatcgccg
     8281 gcataataaa aatttgcttc gtctgtctca aaataaaagc ccgctgcctg tccgccataa
     8341 ctgccatctt caaaagaact cgaatgcact gcatttacca ttttaccgga tccgaaatca
     8401 aaattccata ccccgccgct attcattcgg tgcgcattgg t
//
LOCUS       DAORKW010000097         8428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_229_length_8428_cov_4.508420, whole genome shotgun sequence.
ACCESSION   DAORKW010000097 DAORKW010000000
VERSION     DAORKW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229_length_8428_cov_4.508420"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /gene="cysQ"
                     /locus_tag="PLB46_05640"
     CDS             <1..928
                     /gene="cysQ"
                     /locus_tag="PLB46_05640"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRF76031.1"
                     /translation="TTNQYPTPAKRPKYSVMNCDKTINTLQINIPHWENALEECIQLL
                     KQQEMLEQIDVKKIIAIAEAAGKEIMQIYDTDDFAVTDKSDNSPLTKADKAGNAVIVA
                     ALKEQYPQIPIISEENKMIEYADRKDWNYFWLVDPLDGTKEFIKKNGEFTVNIALVRN
                     GKPVLGVVGIPAKNQMYYAVENKGAYKIDENGVETKLLVNEPKTDEIALIGSRSHPSP
                     EFDAYLKDMESKFATVNFVPAGSSLKFCLVAEGKADVYPRLGPTMEWDTAAGHALVLE
                     AGARVKVYGQEMDLQYNKENLLNPFFIVEKAN"
     gene            complement(906..4505)
                     /locus_tag="PLB46_05645"
     CDS             complement(906..4505)
                     /locus_tag="PLB46_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF76032.1"
                     /translation="MRYFAHNIGLFAFTLLFISGCKLFRSDDHISRASVVIDESRIVK
                     TDVSSGKMFDILTAKSTGLEFLNTIPDNYEDNYWRYVFVYNGGSVNIGDFNNDGLPDI
                     FFTGVHVPHRLFINKGGLQFEDVTAKSGILKGATDWTSGSTVADVNGDGLLDIYICNT
                     RRDNPEERRNKLYINNGDLTFTEAGKSYGLDDASTCSTANFFDYDNDGDLDMYLVTHP
                     MDFINKFKTVYFQKIETQQNLSNKLYRNNGDGTFTDVHLEAGINNHGFGLACSVGDVN
                     EDGWQDIYVANDFGMYDFLYLNKGDGTFTDVSLTAIKRHDVSSMGTDIADIDNDGLLD
                     IFNADIEMEENYTYKTFQVSSQIEIIRTLINAGYGYQKHGNSLKLNNGNGTFSEISRT
                     AGVGVTDWAWSPFFTDFDNDGYKDLFISTGYLQDFNVDETETYSKLRRACRISDSIIY
                     YELVNSIPRNVLDHPNFIYKNNGDLTFADKRDEWGIYYPSVSYGAAAADFDLDGDIDI
                     VCSNANNHPFLYRNNASTLTENNYIKIQLIGYAKNTNGIGAKVRIYYGDEMRYVQHTH
                     VRGYISTTEQVIHFGLGKQNSIDRIEVEWPDGNQQILKDITANQLITIAHKNAKKQVI
                     PIKEKPTPIFVSNTSSGITYEHKENEYDDFLREFLLPHKMSVLGPCIAVGDVDGNQLD
                     DVYFGGSASQMGKLYLQTSPQTFSLSPNQLPAPKLLTDNGGALFFDADNDKDNDLYVT
                     AGGNEFPANDKSYIDYLYLNDGTGKFTLQQQAIPQSYTPKSSVNACDFDADGDMDLFI
                     GGRQYPGAYLKPVSSYILRNDNGKFTDVTAQIAPELLEIGMVTSAIWSDYNNDNKMDL
                     VLCGDWMPFTIFENTGKLFKNISATINDVKTDGWWQSVQGADFDNDGDIDYVLGNFGT
                     NRRYINTPSEVDGRNLPMEAFLSDFDNNKTPDLILCYYQHDQLYPIQLRERLLEQVPS
                     YRKIMPSWDWYGKATVTDIFGEALNDAIHKYAYEFRSVVMRNKGNGKFDILPLPINAQ
                     ISVMMGSVIDDFNQDGFTDILMHGNYYPMDVLFMRHDAGTGLLLLGNGDCTFEGVRGN
                     TSGYWSDGDSRALTTIGCGEKNQPLIVGAINNGKTHVFTWNSGQSIYFQEDEIYALVT
                     YQNGSIQKVESYNGSGYLSQRSRFISLTPTIRFITFVTDTGKQRKVINSLSPL"
     gene            4687..7671
                     /locus_tag="PLB46_05650"
     CDS             4687..7671
                     /locus_tag="PLB46_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76033.1"
                     /translation="MKKITTLVAALTLMLSAYAQADPIPADTAAITPEPINERVQGIQ
                     KRKALETASLVKNLSFTSIGPTIMSGRVTDLDVNPKDASKFYVAYASGGLWYTDNNGQ
                     SFRPIFDNEDVITIGDIAVDWTNNIIWVGTGEVNSSRSSYAGIGVYKSEDMGKTWIYC
                     GLPESHHIGRIAINPRNPENVFVAVLGHLFSPNEERGVYKTIDGGKTWKKTLFVDDNT
                     GVVDLLMDPNVPDFLYATTWHRERRAWNFVEGGQSSDIYKSVDAGETWQLLTTGNSGF
                     PSGNVGRIGITVAPLHNNIVYAVVDNQNPKEQDPALRERELDTSDFNLDSLKIITAAK
                     FATLDETALTKFLKDNKFPEEYTAQSIKAKVATGEYAPTVLTDYLNDGGYVFNLPIKG
                     CEVYRSDDGGTSWYKTHEGYLDGAFFTYGYYFATIKVSPKNDNKILVSAFNALLSEDG
                     GKTFKNIDGPNVHPDHHAAYFNPTNDKHIIVGNDGGVNITYDNGANWFFANNPAVGQF
                     YSVAVDNATPYNVYGGLQDNGVWWGPSDYEKSNYWQAAGKYPYQPLYGGDGMQVQVDT
                     RDNATVYTGYQFGYYARVNNATAQEEIAIRPSHKFGESPLRYNWQTPILLSRHNQDIL
                     YYGSNKFHRSMNKGENMTTLSGDLTNGKKTGDVPYGTITTIAESPKRFGLLYCGTDDG
                     NIWVSKDGGYSWNKINVFIKKELPPVKSKTPPPVFNAIPDGLWVSRVAASNHNEGTVY
                     ASLNGYRYDNFLPYLFVSTDYGATWSEIGKDLPYEPINVVKEDVKNPNIIYVGTDNGL
                     YVSINGGKNFMAFNGNLPRVAVHDIAIQERDNEMVIATHGRSLYKVGLDEIQTLDFIS
                     NKDIHIFATEKFQYDENAGKQFNVFDKPYEPTYKFPYYVKNAGITTITIATIDGTVIH
                     EFTDNSEAGLNYLDYALTFDAKYKSAYEEYLNGLPVEGYREVANIKAADNKLFYLMPG
                     KYIIKFTAESLTTVQENFEIVK"
     gene            7970..8212
                     /gene="rpmB"
                     /locus_tag="PLB46_05655"
     CDS             7970..8212
                     /gene="rpmB"
                     /locus_tag="PLB46_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRF76034.1"
                     /translation="MARVCDLTGKKTISGNNVSHSNRKTRRKFYPNLQVKRFYIPEED
                     RWVTLKVATTTMKTINKKGISAYLKELRQAGKTINI"
     gene            8224..8412
                     /gene="rpmG"
                     /locus_tag="PLB46_05660"
     CDS             8224..8412
                     /gene="rpmG"
                     /locus_tag="PLB46_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRF76035.1"
                     /translation="MAKKSKGNRIQVILECTEQKNTDVPGTSRYITTKSRRNTPDRIE
                     MKKYNPNLKKVTAHKEIK"
BASE COUNT         2787 a         1563 c         1487 g         2591 t
ORIGIN      
        1 cacaaccaac caatatccaa ctcccgcaaa acgcccgaaa tattcggtaa tgaattgcga
       61 taaaacgata aatactttac aaataaatat cccgcattgg gaaaatgcat tggaagaatg
      121 catacaacta ttaaaacaac aagaaatgtt agaacagatt gacgttaaaa aaattattgc
      181 gattgctgaa gcagcaggta aagaaattat gcaaatttat gatactgacg attttgcagt
      241 taccgataaa agtgataatt caccattaac caaagctgac aaagcaggta atgcagttat
      301 tgttgctgca ttaaaagaac aatatccgca aattccgatt atcagcgaag aaaataaaat
      361 gattgaatat gctgaccgca aagactggaa ttatttttgg ttagtagacc cgcttgatgg
      421 cactaaagaa tttattaaaa aaaatggcga gttcacggta aacatcgcat tagtgcgtaa
      481 tggcaaaccg gtattaggcg ttgttggaat tcctgctaaa aatcaaatgt attatgcagt
      541 agaaaataaa ggtgcttata agattgacga aaacggtgtg gaaacaaaat tgcttgtaaa
      601 tgaaccaaaa actgatgaaa ttgcattaat cggaagccgt tcacatcctt cacctgaatt
      661 tgatgcttat ttaaaagaca tggaaagcaa atttgctact gtaaattttg ttcctgcagg
      721 tagttcatta aaattttgtt tagttgcaga aggcaaagct gatgtttatc ctcgtctagg
      781 gccaaccatg gaatgggata ctgctgctgg tcatgcttta gtattagaag caggcgccag
      841 agtaaaagta tatggtcagg aaatggattt acaatacaat aaagaaaatt tattaaatcc
      901 gttttttata gtggagaaag cgaattaatt acttttcgtt gtttaccagt atcggtaaca
      961 aatgttatga atcgtatagt tggagttagt gaaataaatc tcgaacgctg agataaataa
     1021 ccgctcccat tatacgattc tactttttgg atacttccat tttgataggt caccaaggca
     1081 taaatttcat cctcctgaaa ataaatgctt tgtccgctat tccaggtaaa tacatgcgtt
     1141 ttgccattgt tgatggcgcc aactattaat ggctgatttt tttcaccaca acctattgta
     1201 gttaaggcac gagaatctcc gtcactccaa tagccgctcg tattgccccg caccccttca
     1261 aaagtgcagt ctccattacc taataataat aatccggttc cggcatcatg acgcataaat
     1321 aatacatcca ttggataata attgccatgc attaaaatat cggtaaaacc atcttgatta
     1381 aaatcatcaa taacactgcc catcattaca cttatttgtg catttatggg taatggtaaa
     1441 atgtcaaatt taccattacc tttattgcgc atgactacac ttctaaattc atacgcatat
     1501 ttatgaatgg catcatttaa ggcttcacca aaaatatctg ttactgttgc tttgccatac
     1561 caatcccaac ttggcataat tttccgatag gagggaacct gttctaataa acgttcacgt
     1621 aattgaattg gatataattg atcatgctga tagtaacaaa gaattaaatc cggtgttttg
     1681 ttattatcaa aatcggatag aaatgcctcc ataggtaaat tacgtccatc aacttctgaa
     1741 ggcgtattga tgtatctcct gttggtacca aaattgccta atacataatc gatatcacca
     1801 tcattatcaa aatcggcacc ttgtacactt tgccaccaac catcagtttt tacatcgttt
     1861 attgttgctg aaatattttt aaataattta cccgtattct caaaaatggt aaatggcatc
     1921 cagtccccac ataatactaa atccatttta ttatcattat tgtaatcgct ccaaatagcg
     1981 gaagtcacca taccaatttc cagtaattcc ggagcaattt gagctgtcac atctgtaaat
     2041 tttccattat catttcgtaa aatataactg cttaccggtt ttaaataagc acccgggtat
     2101 tgtcgtcccc caataaataa atccatatcg ccatcagcat caaaatcaca ggcattcaca
     2161 ctcgattttg gggtataact ttgtggtatt gcttgttgct gcaaggtgaa ttttccggtg
     2221 ccgtcattca ggtataaata atcaatgtaa cttttgtcgt tggcaggaaa ttcatttcct
     2281 cctgcagtta catataaatc attatcctta tcattatctg catcaaaaaa taatgcgcct
     2341 ccattatcgg ttaataattt tggtgcagga agttggtttg gtgataaact gaatgtttgt
     2401 ggggaagtct gtaaatagag ttttcccatc tgacttgcac ttccaccaaa atatacatca
     2461 tccaattgat tgccatctac atcaccaaca gcaatacaag gacctaaaac actcatttta
     2521 tgtggcaata aaaattcgcg caaaaagtca tcatattcat tttccttatg ttcgtaagtt
     2581 attccggaag atgtgttgga aacaaaaatc ggtgtgggct tttctttaat aggtataacc
     2641 tgtttttttg catttttatg tgctattgta atcaactgat tggctgtaat atcctttaaa
     2701 atttgctgat tgccatcagg ccattccact tcaattctat ctatactatt ttgtttgcct
     2761 agtccaaaat gtattacttg ttctgtggta gaaatatagc ctcttacatg ggtgtgttgc
     2821 acatatcgca tctcgtcacc atagtagatg cgcactttcg cgccaattcc atttgtattt
     2881 ttggcataac ctattaattg tatttttata taattatttt ccgttaatgt agaagcatta
     2941 ttgcgatata aaaacggatg gttatttgca ttagaacaca caatgtcaat atcgccgtcc
     3001 aaatcaaaat ccgcggcagc tgcaccatac gaaacagatg gataataaat tccccactca
     3061 tcacgtttgt cggcaaaagt taaatcacca ttatttttat aaataaaatt gggatgatcc
     3121 aatacatttc ttggaattga attcaccaat tcataataaa taatgctatc actaattcga
     3181 catgctctgc gcagtttgct ataggtttct gtttcatcaa cattaaaatc ctgcaggtag
     3241 ccggttgaga taaataaatc tttgtatcca tcattatcaa aatcagtaaa aaacggactc
     3301 caggcccaat ctgttacacc aactcctgct gtgcgcgata tttccgaaaa ggtgccatta
     3361 ccattattta attttaatga attaccatgt ttctgataac cataaccggc atttattaag
     3421 gttctgatta tttcaatctg actcgatacc tgaaatgttt tgtaagtata attttcctcc
     3481 atttcaatat cagcattaaa aatgtccagc aaaccatcat tgtcaatatc tgcaatatcg
     3541 gtgcccattg aactcacatc atgtcgttta attgctgtta acgaaacatc agtaaatgtg
     3601 ccatcgccct tatttaaata taaaaaatcg tacatgccaa aatcattggc aacataaata
     3661 tcctgccaac catcttcgtt tacatcacca accgaacacg caagtccgaa accatgatta
     3721 tttattcctg cttcaaggtg cacatctgta aaggtgccat cgccattatt gcggtataat
     3781 ttattgctta aattttgttg tgtttcaatt ttttgaaaat atactgtctt gaatttattg
     3841 ataaaatcca tgggatgggt aactaaatac atatctaaat caccatcatt gtcataatcg
     3901 aagaaattgg ctgttgaaca tgttgatgca tcatctaacc cataggattt tcctgcttcg
     3961 gtaaatgtta aatcaccatt gttaatatat aatttattgc gcctttcttc aggattatcg
     4021 cgacgggtat tgcagatata aatatctaat aatccatctc catttacatc agcaaccgta
     4081 gaaccggatg tccaatcggt agcacctttt aaaatgccgc tttttgctgt tacatcttca
     4141 aattgtagtc cacccttatt aataaataaa cgatgcggca catgcacacc ggtaaaaaaa
     4201 atatcaggga gcccatcatt attaaaatcg ccaatattta cactgcctcc attatataca
     4261 aacacatatc gccaataatt atcttcataa ttatccggaa tggtattgag aaattccaac
     4321 cctgtcgatt tagcagtcaa tatatcaaac atttttccgc ttgacacatc tgtttttaca
     4381 atccgtgatt catctataac tactgatgct cgtgatatat ggtcatcact tcgaaatagc
     4441 ttacagcctg aaatgaataa tagtgtaaaa gcaaataaac cgatgttgtg tgcgaaatat
     4501 ctcattagaa aggttaaaat aatacaattt taaatttttt gtaaatatca tgctaaaaaa
     4561 taccgatttt gtgacatgca attctttgtt aattttttga attttgagta aaattttgtg
     4621 acttcgcctt ttataagaag aaagacaggc ctatttttaa gccatcgtta gcccaaacca
     4681 gaaattatga aaaagatcac aacacttgta gcagccttaa cccttatgct ttctgcctac
     4741 gcacaggcgg accccatacc ggcagatact gctgcaataa cacctgaacc tattaatgaa
     4801 cgtgtgcagg gaattcagaa aagaaaggcc ctcgaaaccg catctttagt taaaaacctg
     4861 agtttcacca gtataggacc taccattatg agcggtcgtg tgacagactt agatgtcaat
     4921 cctaaggacg cttctaaatt ttatgtggca tatgccagcg ggggtttatg gtatactgat
     4981 aacaacggac aatcgtttag accgattttc gacaatgaag atgttattac aatcggtgac
     5041 atagcagttg actggactaa taacataatt tgggtgggaa ccggtgaggt aaatagctct
     5101 cgttccagtt acgctggtat aggcgtatat aaatcggaag acatgggtaa aacctggatt
     5161 tattgtggat tgcctgaatc acatcatatt ggtcgtattg caatcaatcc aagaaatcct
     5221 gaaaatgtat ttgttgccgt gttaggtcat ttgttttcac ctaacgaaga acgtggcgta
     5281 tacaaaacta tagatggtgg taaaacctgg aaaaaaacat tatttgttga tgataatacc
     5341 ggtgtcgttg atttattaat ggaccctaat gttcctgatt tcctttacgc aacaacctgg
     5401 catcgtgaac gccgtgcatg gaattttgtg gaaggcggac aatcatccga tatttataaa
     5461 tcagtggatg ccggcgaaac ttggcaatta ttaactaccg gaaattccgg tttcccttca
     5521 ggtaatgttg gacgtattgg tattacagtt gctccactgc ataataatat tgtgtatgca
     5581 gtcgtagata atcaaaaccc gaaagaacaa gaccctgcct tgcgtgaacg tgaactggat
     5641 acatcagatt ttaatctcga cagtctaaaa ataattaccg ctgcaaaatt tgctacatta
     5701 gatgaaacag cgcttacaaa atttttaaag gataataaat ttcctgaaga atatactgct
     5761 caatccatta aagcaaaagt ggctaccggc gaatatgcac caacggtatt aaccgattat
     5821 ttaaacgacg gtggttatgt atttaattta ccaattaaag gttgtgaagt ttaccgcagc
     5881 gatgatgggg gaacctcatg gtataaaacc catgaaggct atttagatgg tgcctttttt
     5941 acttatggat attatttcgc aactattaaa gtaagtccta aaaatgataa taaaattctg
     6001 gtgtctgctt ttaatgcatt attaagtgaa gatggcggaa aaacatttaa aaatattgat
     6061 ggacctaacg tacatccaga ccatcatgcg gcttatttta atcctacaaa cgacaaacat
     6121 attattgtcg gaaacgacgg tggtgtaaat ataacttacg acaatggtgc caattggttt
     6181 tttgccaaca accctgcagt aggtcaattt tattcagtgg ctgtagataa tgcaacacca
     6241 tataatgtgt atggcggatt acaagataat ggcgtatggt ggggaccttc tgattacgaa
     6301 aaaagtaatt actggcaagc tgccggaaaa tatccgtatc aaccgttata cggtggcgat
     6361 ggtatgcagg tgcaggttga tacccgcgac aatgctactg tgtatacagg ttatcaattt
     6421 ggttattatg cccgcgtaaa taatgcaact gcgcaagaag aaattgctat tcgtccttca
     6481 cataaatttg gtgaatcacc attgcgttat aactggcaaa caccaatttt attaagtcga
     6541 cataatcagg atatccttta ttatggttca aataaatttc atcgctcaat gaacaagggc
     6601 gaaaatatga ccacattaag tggcgattta accaatggca aaaaaaccgg tgatgtacct
     6661 tacggaacaa ttactaccat tgctgagtca ccaaaacgtt tcggattatt gtattgcgga
     6721 actgatgatg gaaatatttg ggtaagtaaa gacggcggat attcttggaa taaaataaat
     6781 gtatttatta aaaaagaatt acctccggta aaaagtaaaa cacctcctcc ggtatttaat
     6841 gcaattcctg acggactttg ggtgagccgt gttgcagcaa gtaatcacaa tgaaggaact
     6901 gtgtatgctt cattaaacgg ataccgatat gataactttt taccctattt atttgtaagt
     6961 accgattacg gtgcaacctg gtccgaaata ggtaaagatt taccatacga accaattaat
     7021 gtggtaaaag aagacgttaa aaatccgaat attatttatg tgggaaccga taatggttta
     7081 tacgtttcaa taaatggcgg aaaaaatttc atggcattta atggtaatct gccaagagtt
     7141 gctgttcatg atattgctat tcaggaacgc gataacgaaa tggtaattgc tactcacgga
     7201 agaagtttat ataaagttgg gttagatgaa attcaaacat tggattttat ttcgaataaa
     7261 gacatacata tttttgctac cgaaaaattc caatatgatg aaaatgcagg taaacaattt
     7321 aatgtattcg ataaacctta tgaacctacc tacaaatttc cttattacgt gaaaaatgca
     7381 ggtattacta ccatcacaat tgccactatt gatggtactg ttattcatga atttacggac
     7441 aatagtgagg ccggtttaaa ttaccttgac tatgcactta ccttcgacgc aaaatacaaa
     7501 tctgcgtatg aagaatattt gaatggttta ccggttgaag gttaccgaga agtggccaac
     7561 ataaaggcag cggacaataa attattttat ttgatgccgg gtaaatatat catcaaattt
     7621 accgctgaaa gtttgacaac cgttcaagaa aactttgaga ttgtgaagta ataaataaaa
     7681 tccttaaacg aaaaaaggct gtctctaagg cagctttttt tttaccgggt gggtaaaaca
     7741 aggataattt gcgttttaag tgactttttt aataaaaaat agataccttt ccttaggatt
     7801 tcgctaaccg gaagccttga aatcaaagac ttattagctt ctaaaacccc gtttttttgc
     7861 tttctgagcc agtcccaggg gttattaaca ccaattattc agcaaaatcg gcgatttttt
     7921 tgggtttttc attaaaacat ccgatatttg cactctcatt ttaaagaaca tggcaagagt
     7981 ttgtgattta accggcaaaa aaacgatcag tggaaacaac gtttctcact ctaatcgtaa
     8041 gacacgccgc aagttttacc cgaatttgca ggtaaaacgt ttttatattc ctgaggaaga
     8101 ccgttgggta actttaaagg tagctactac taccatgaag actatcaaca aaaaaggtat
     8161 cagtgcatac ctgaaagaat tgcgtcaagc aggtaaaact attaacattt aaatagctga
     8221 tttatggcaa agaagagcaa aggcaaccgg atccaggtaa ttctggagtg caccgaacaa
     8281 aagaacactg atgttccggg aactagccgt tatatcacga ctaaaagtcg tcgtaataca
     8341 ccggacagaa tcgaaatgaa aaaatataat ccgaacttga aaaaggtgac ggctcacaaa
     8401 gaaatcaaat aaacccaaaa ccagaaac
//
LOCUS       DAORKW010000098         8414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_230_length_8414_cov_4.486900, whole genome shotgun sequence.
ACCESSION   DAORKW010000098 DAORKW010000000
VERSION     DAORKW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230_length_8414_cov_4.486900"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PLB46_05665"
     CDS             <1..547
                     /locus_tag="PLB46_05665"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRF76036.1"
                     /translation="VTKGSAIDGYFANPDIKILFPEEAAVVESVVGLIPGGDILIDEF
                     VQSVNRAAEDAADEATPIIKDAIVNITFDDAMTILNGSDTAATNYLRLKTFSDLYTAF
                     KPDIQNSLEGVGAQQAWEEIIDLYNTIPFVEDVNTDLADYSTNKGLDGLFILLGQEEG
                     KIRTDVEHQVTSLLQEVFGGN"
     gene            677..1381
                     /gene="pnuC"
                     /locus_tag="PLB46_05670"
     CDS             677..1381
                     /gene="pnuC"
                     /locus_tag="PLB46_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HRF76037.1"
                     /translation="MFFILLMNLSNCLHDSVLFMTIPEIIAVTTGILSVWFAKKENIL
                     VYPIGIISVLLYVYICLEVKLYADAAINAYYFVVSMYGWYYWKNGKTTKLSKNKTEQN
                     QFDQVFANDQNITFSGKKAVITWNKKEENLYYVLGTIFLTAFLGYMLNEFTDSTVPYW
                     DGGTTAIFCVAMILMALKKIENWIYWIIGDVICIPLFFSKGLCLSSFQYLIFTGIAIA
                     GLMVWMKKYNDLKTTS"
     gene            1378..1902
                     /locus_tag="PLB46_05675"
     CDS             1378..1902
                     /locus_tag="PLB46_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF76038.1"
                     /translation="MIHKIVFTGPESCGKSTLVELLAQKLQLPFVPEMARPYINNLNR
                     KYTYDDLLSIAMLQMSEEERIKSMHPPLLICDTDLLTIKIWCDDKYQRCEHWLNHAWV
                     DRTPGMYFLCAPDFPWQPDPQREDPNRRDALYQVYKDHMVKYKLRFIELKGTVEQRLA
                     TVMKHLEGDSQQLY"
     regulatory      1921..2019
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2068..4485
                     /locus_tag="PLB46_05680"
     CDS             2068..4485
                     /locus_tag="PLB46_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF76039.1"
                     /translation="MKKFLFFSFFIFLYGAISSQITVTGNVSIIDEKTPISDVAVYFV
                     NNDVLVFTNQNGDFLAVLDSFEMPTAQHIAFMSETLQYELVANDMNTTGKQPYSEIKM
                     YLYPKNYIFLPVKINANSWIQKAPLSIKVIKGAQLNENNFGEDMPFLLESTPSSVVTS
                     DAGNGVGYTGMRIRGSDATRVSVTVNGVPYNDAESQQTYWVDLPDFASSVDDIQIQRG
                     VGTSTNGISAFGAAININTNNLSKSPFVKTGYYTGSFNLSKKMIQFGSGLIKNHWTIE
                     GRYSDVYAEGYIDRAYADLNSYFITAAYQSEKYKSVINVFSGNEITYQSWGGVPAEII
                     DTNRTYNPYTYENQTDNYIQTHYQWHNSFVVNDEDFLKVTLNYTKGKGYYEQYETDQT
                     FSDYGAEDVIINEDTISVTDLISRKWLDSDFGGMFLQYEHNINNNTKFNIGGAYYVHV
                     GDHFGNIIWSEWAATLPHNYEWYRNDALKQDGNIFAQYIYQNSKINWLIDLQVRNVNY
                     RFEGLDQFAVPLEQQVNHLFFNPKAGITFSHGNLFETYLFGGVSGKEPNRDDFVESSP
                     ESRPAPEYLYNAEIGERLRFKGWQFEANLYMMYYKNQLVLTGEINDVGAYTRTNIPES
                     YRTGIEIAWGKSMFSNKLTWEGNVAFSQNKITTYTEFVDNWDTGEQTAIVYNNTDIAF
                     SPNLIAFNKLKYQLIAFGNTTFNSQFDIWVSSKYVGEQFADNTGDVARMLDAYLVHDA
                     GISYNLQKSKKQLLAMTFNLQNVTNVEYESNAWVYRYIYENAQNQLMGYYPQALINWN
                     AGLVLSF"
     gene            4572..6500
                     /locus_tag="PLB46_05685"
     CDS             4572..6500
                     /locus_tag="PLB46_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRF76040.1"
                     /translation="MRKSLLTISLAIATAVLFTALVVPPVDTDYDWMVEGNDVDSEFG
                     FWIDGGGDVNGDGYDDVIIGASGYEHDFIGEGAVFIYFGSPSGLSLEPDWVQYGEHDS
                     SGYGRCVSIKGDVNNDGYADVLVGAHEFTGTRVNEGKVYLYLGGPDGPSTTPYWTKVG
                     GRKGAKLGEAVVFAGDLNNDNYDEICIGAHGWDDDEVLGDLGNKAGKFWIFNGNADGI
                     NPVAFMSCVGIVEDANLGVSMDRAGDVNGDGFNDLHIGGYIFLIGDGMLCTFHGSAVG
                     PDGIPDFMVAGGAMDTSFFAINLSSAGDVNGDGFDDVVIGSPRYDALGVYQSGKLQVY
                     LGSEGGLQPEIAFTAHGTQYDERFAFNVNEAGDLNHDGHDDLLVGSKYYDGGGLQNTG
                     KAEIYLGGPQGLQKTPTWSFVGLDSGNVVGTNLANAGDVNGDGYDDIIVSGDEYCGDL
                     LREGAVYMFYGQPQKCEAPQNLTPYFVTPNSVTIGWDWLYGSQKYKFYMRRTDGVGTE
                     YKIITQDSLLFIGGLTSGKEYTAYVAAKCDGGWTDKSEIITFTTPGHKEGELSGMQIY
                     PSPTSDKININLGPATGPVMIKIVNLAGEVVFENQYQIDESHTVIVVNEVKSLPVGNY
                     FVLVETSNSKLTKQFVKQ"
     gene            6719..7039
                     /locus_tag="PLB46_05690"
     CDS             6719..7039
                     /locus_tag="PLB46_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRF76041.1"
                     /translation="MAQQYNLLRILRGQYPNPASVNLLIERMLDKQSNASRLVDKLDA
                     KGLVKRKKCKEDKRQVDVVLSTKGVQLLDAIDKEIDNLDNIFTNISKAEAKKMSELLD
                     KMRG"
     gene            7039..7455
                     /gene="arfB"
                     /locus_tag="PLB46_05695"
     CDS             7039..7455
                     /gene="arfB"
                     /locus_tag="PLB46_05695"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRF76042.1"
                     /translation="MSAINLIPDLSAEFTFKAVRSGGKGGQNVNKVSTKVELYFTISN
                     SQLLSEAQKEVLIQKLQHLLNSEGELRIVCEEARSQLKNKQLVIEKLYALFEKALTPV
                     KKRVKTKTPKSVIAKRIKDKKIAGEKKSMRKKPDLD"
     gene            complement(7461..8183)
                     /locus_tag="PLB46_05700"
     CDS             complement(7461..8183)
                     /locus_tag="PLB46_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF76043.1"
                     /translation="MCRFALILLLLISGMVVTAQQTTRVYLIPGQGSDYRIYKNIQFD
                     AHLDTIHVHYITPLPFESMSAYAKRLSAQIDTTTPFYIIGVSLGGMLATEMYDIVHPQ
                     KIIVISSATDSTEIPEMYQFFHQYPLHRYIPASVFKYSSFIMQPMYEQDRALERATCN
                     AMLWDKEKIFVKRATEMIVSWQRKPEDNTGKNIIHIHGDNDNTLPIKNITADYIIPGG
                     SHMMVLTKAEVITPILNKELKQ"
BASE COUNT         2724 a         1412 c         1697 g         2581 t
ORIGIN      
        1 agttaccaaa ggttccgcaa ttgatggcta ttttgctaat cctgatatta aaatattatt
       61 ccctgaagaa gctgctgttg ttgaatcggt agttggcctt attcccggag gtgatatact
      121 tattgatgaa tttgtgcaat cagtaaaccg cgctgctgaa gatgcagcag acgaagcaac
      181 ccctataatt aaagatgcaa ttgtcaatat tacttttgat gatgctatga ctattttaaa
      241 tggttcagat acagcggcaa ctaattatct ccgactaaaa acattttccg atttatatac
      301 ggcctttaaa ccggatatac aaaactcatt ggaaggggtt ggcgcacaac aggcttggga
      361 agaaattatt gatttatata ataccattcc ttttgtggaa gatgtaaaca ccgacctggc
      421 tgattattca acgaataaag gactggatgg tttatttatc ctattaggtc aggaagaagg
      481 aaaaatcaga actgatgttg aacatcaggt aacaagtttg ttacaagaag tgtttggcgg
      541 aaattaatat cagttaacta tctgataact aaacaggtag tatttaggtt aaggtattgg
      601 aaacacttgc tatttgtgtt ttacaagctt caaactaatt gtaaatactg taaaaactgc
      661 acaaattgtt gcagttttgt tttttatact actgatgaac ctgagcaatt gtttacatga
      721 ttctgtgtta ttcatgacca taccggaaat aattgccgtt actaccggaa ttttaagtgt
      781 ttggtttgcg aaaaaagaga atatactcgt ttaccctata ggcatcataa gtgtgttatt
      841 atacgtttat atctgcctgg aagtgaaact atatgccgat gcagcaatta atgcttatta
      901 ttttgttgtg agtatgtatg gctggtatta ttggaaaaat ggcaaaacaa cgaagttatc
      961 aaaaaacaaa actgaacaaa accaatttga ccaggtgttt gccaatgatc aaaatataac
     1021 tttttccgga aaaaaggctg ttattacctg gaataaaaaa gaggaaaatt tatactatgt
     1081 tttgggaaca atatttttaa cagcattttt aggatatatg ctgaatgaat ttaccgattc
     1141 tactgttcct tattgggatg gaggaaccac agccattttt tgtgtggcga tgattttaat
     1201 ggcactgaaa aaaattgaaa actggatata ctggattatt ggcgatgtaa tttgtatccc
     1261 attatttttt tcaaaagggt tatgtttaag ttcgtttcag taccttatat ttacaggaat
     1321 agccattgca ggtttaatgg tttggatgaa aaaatataac gacttaaaaa ctacttcatg
     1381 atacataaaa ttgtgtttac cggccccgaa tcatgcggta agtctacatt ggtagaatta
     1441 ttggcacaaa aactacaatt gccttttgtc cctgaaatgg caaggcctta tatcaacaac
     1501 ctcaatagga aatatacata cgatgacctg cttagtattg ccatgttgca aatgagcgag
     1561 gaagaacgta taaaatctat gcaccctcca ttactgattt gtgatacaga tttgctcacg
     1621 attaaaattt ggtgcgatga taaatatcaa cgctgcgagc attggctgaa tcatgcgtgg
     1681 gtggacagaa caccgggtat gtattttttg tgtgctcctg attttccttg gcaaccagac
     1741 ccgcaacgtg aggacccgaa cagacgagat gctctttatc aagtgtataa agaccatatg
     1801 gtgaaatata aattgcgttt catcgaatta aaaggcactg tggaacagcg tttggccact
     1861 gtaatgaaac atcttgaagg cgatagtcaa caattatatt aaaatcttgt tgtaactttg
     1921 taacatctca tggggtgttg tttgttcgca aacgactgag attaaaccca tcgaacctgg
     1981 ccgggtaatt ccgggtaggg attgaggaaa attttatttc tgccggcaaa ggctccattt
     2041 gcaggtttca aactgtaaac ctttgctatg aaaaagtttt tatttttttc attttttata
     2101 ttcctgtatg gtgctatatc atctcagata actgtaaccg gtaatgtttc gattattgac
     2161 gaaaaaacgc caattagcga tgtcgccgta tatttcgtaa acaatgatgt attggtgttt
     2221 accaaccaaa atggcgattt tttagccgtg ctcgattcat ttgaaatgcc aactgcgcag
     2281 catattgcat ttatgagcga aacgctgcaa tatgaattgg tagctaacga tatgaatacc
     2341 accggaaaac aaccttactc tgaaattaaa atgtatttgt atccaaaaaa ttatattttt
     2401 cttccggtaa aaatcaatgc caattcgtgg atacaaaaag caccgttatc cattaaagta
     2461 ataaaaggtg cgcagttaaa tgaaaataat tttggtgaag acatgccatt tttattagag
     2521 tcaacaccat cttcagttgt tacttccgat gctggtaatg gcgtgggata taccggcatg
     2581 cgtattcgcg gaagtgatgc tacacgagtg agtgttacag tgaatggtgt gccttacaat
     2641 gatgcagaat cacaacaaac atattgggtc gatttacccg attttgcttc cagtgtagac
     2701 gatattcaaa ttcaacgcgg agttggcact tcaacaaatg gaattagtgc atttggcgca
     2761 gctattaata tcaataccaa taatttaagt aaatctcctt ttgtgaaaac agggtattat
     2821 accggctcat ttaatctctc caaaaaaatg attcaatttg gtagtggatt aattaaaaat
     2881 cactggacta tcgaagggcg ttattctgat gtgtatgctg aaggatatat cgaccgggct
     2941 tatgctgatt taaacagtta ttttatcact gctgcttatc agtccgaaaa atataaatcg
     3001 gtaattaatg tattttccgg taatgaaatt acttatcagt catggggtgg tgtgcctgct
     3061 gaaattattg acacaaacag aacgtataat ccttatacct acgaaaatca gaccgataat
     3121 tatatccaaa cacattatca atggcataac agttttgtag taaatgatga agattttttg
     3181 aaagtaacat taaattatac caaaggcaaa ggatattacg aacaatatga aactgaccaa
     3241 acatttagtg attatggtgc agaagatgta ataataaatg aagacaccat ttctgtcact
     3301 gatttaattt cacgcaagtg gctcgatagc gattttggcg gcatgttttt acaatacgaa
     3361 cataatatca ataataatac aaaattcaat attggcggcg catattatgt acatgttgga
     3421 gatcattttg gcaatattat atggagcgaa tgggcagcaa ctttaccaca taattatgaa
     3481 tggtatcgca acgatgcatt aaaacaagac ggcaatattt ttgcacaata tatatatcaa
     3541 aacagtaaaa taaattggtt gattgacctg caggtgcgca atgtaaatta tcgctttgaa
     3601 ggcctcgatc aatttgcggt tccactggaa caacaagtaa atcacttgtt ttttaatcca
     3661 aaagcaggta tcacattttc acacggcaat ttatttgaaa cctatttatt tggaggggtt
     3721 tctggtaaag aacctaatcg agatgatttt gtagaatctt ctccggaaag tcgccctgcg
     3781 cctgaatatt tatataatgc cgaaataggt gaacgtttgc gatttaaagg atggcaattt
     3841 gaagcaaatt tatatatgat gtactacaaa aatcaattag tactcaccgg agaaattaat
     3901 gatgttggag cctataccag aacaaatatt cctgaaagtt acagaacagg aattgaaatt
     3961 gcctggggaa aatcgatgtt ttcaaataaa ttaacttggg aaggtaatgt cgccttcagc
     4021 caaaataaaa ttaccactta caccgaattt gtcgataatt gggataccgg agagcaaaca
     4081 gcaattgttt acaataatac agatattgca ttttcaccca acctgattgc ttttaataaa
     4141 ttaaaatatc aattaatagc ttttggaaat accaccttta attctcagtt cgatatttgg
     4201 gtatcctcga aatatgtggg cgaacaattt gcagataata caggtgatgt tgcgcgtatg
     4261 ttagatgctt atttagtgca tgatgcaggt attagttaca acttacaaaa atcgaaaaag
     4321 cagttacttg caatgacatt taatctgcaa aacgttacca atgttgaata cgaaagtaac
     4381 gcgtgggtgt atcggtatat ttacgaaaat gcacaaaacc agctcatggg ctattatcca
     4441 caagccttaa tcaattggaa tgccggctta gtactgtcat tttaaaacct tttacactac
     4501 ctgctgtaat tgattaaaat cattttggtt aagcttaaaa ttccaattac ctttaatcag
     4561 atttttgagc tatgaggaaa tctttactta ccatatcact tgctattgct actgctgtat
     4621 tgtttacagc attggtggtg ccaccggttg ataccgatta cgattggatg gtcgaaggaa
     4681 atgatgttga ctctgaattc ggattttgga tcgacggcgg tggagatgta aatggcgatg
     4741 gttacgatga tgtaattatt ggcgcatcgg gttatgagca tgatttcata ggtgaaggcg
     4801 ctgtatttat ttatttcggt tcaccttcag ggctttcact tgagccagac tgggtgcaat
     4861 atggtgaaca cgatagtagc ggttatggcc gttgcgtttc aattaaaggt gacgttaata
     4921 atgatggtta tgcagatgta ttggttggag ctcatgagtt tactggaaca cgggtaaatg
     4981 agggaaaagt gtatttatac ctcggtggtc ctgatgggcc ttctacgaca ccctactgga
     5041 ctaaagttgg aggtagaaaa ggtgctaaat taggcgaggc agttgttttt gccggagatt
     5101 tgaataatga taattacgat gagatttgta ttggtgccca tggttgggac gatgatgaag
     5161 tgttaggcga tttaggtaat aaggcaggta aattttggat atttaatggc aatgccgatg
     5221 gcataaaccc tgttgcgttt atgtcctgcg ttggaattgt tgaagatgcc aaccttggcg
     5281 tatctatgga tcgcgcgggt gatgtaaatg gcgatggatt taacgacctg catattggcg
     5341 gatatatttt ccttataggg gatggcatgt tgtgtacgtt tcacggcagt gctgttgggc
     5401 cggatggcat acctgatttt atggtagccg gcggcgcaat ggatacatca ttttttgcga
     5461 tcaatctatc ttcagctgga gatgtaaatg gtgatggttt cgatgatgta gtaattggtt
     5521 caccaagata tgatgccctc ggtgtatacc agtccggtaa actccaagtc tacttaggaa
     5581 gtgaaggagg attacagcct gaaatagcct tcaccgccca tggcacacaa tacgatgaac
     5641 ggtttgcatt taatgtaaac gaagctggcg atttaaatca tgatgggcat gatgatttat
     5701 tggtgggttc taaatattat gacggtggtg gattacaaaa taccggcaaa gcagaaatat
     5761 atctcggtgg cccacaagga ttgcaaaaaa caccaacttg gtcgtttgta gggttggata
     5821 gcggaaatgt ggtaggtact aatttggcca atgcaggaga tgtaaatggc gatggatacg
     5881 atgatatcat tgtttcaggt gatgaatatt gtggcgattt actaagggaa ggtgctgttt
     5941 atatgtttta cggtcagccg caaaaatgtg aagcaccaca aaatctcaca ccatattttg
     6001 ttacacctaa ttctgtaact attggctggg attggttgta tggctcgcag aaatacaaat
     6061 tttacatgcg tagaaccgat ggtgttggca ctgagtataa aattataacc caggattcgt
     6121 tgttgtttat tggtgggctg acttccggaa aagagtatac agcttatgtt gcagcaaaat
     6181 gtgatggtgg ttggaccgat aaatcggaaa ttattacgtt tacaactccg ggacacaaag
     6241 aaggtgaatt atcaggcatg caaatttatc cgtcaccaac ttctgataaa atcaatatca
     6301 atttaggccc tgctaccggt ccggtaatga ttaaaatagt gaatttagcc ggagaagtag
     6361 tcttcgaaaa ccaataccag attgatgaat ctcatactgt aattgtagtc aatgaggtga
     6421 aaagccttcc tgtaggaaat tatttcgttt tggttgaaac cagcaatagt aaattgacta
     6481 aacaattcgt taaacaataa gactgtgcac aaattatagc ggcaattgta gcgactattg
     6541 ccgttttttt atacttttag acgacctaac tacctgtcaa ctaatcatct atgaaactag
     6601 aagaagaaat caaacaacag aaatttaagg atgaatacca tcgtttattg gtaaatatca
     6661 tgtttaccgg aagctggtta aacatccgac aaatacactt tttcaaacaa tttgatttat
     6721 cgcccaacaa tataacctac tgcgtatttt gcgtggccaa taccctaatc ctgcaagtgt
     6781 aaacctgctt atcgaacgta tgctcgataa acaaagtaat gcttcacgat tggtcgacaa
     6841 actggatgct aaaggattag tgaaaagaaa aaaatgtaaa gaagataaac gtcaggttga
     6901 cgttgtatta tccacaaaag gtgttcaact gctggatgcc attgataaag aaattgacaa
     6961 tttggataat atttttacca atatatcgaa agcagaggcg aaaaaaatga gcgagttgct
     7021 cgacaaaatg cgtggttaat gtccgccata aatctcatac ccgatttatc tgctgaattc
     7081 acttttaaag ctgtccgcag cggaggtaaa ggtggtcaaa atgtaaataa agtatctact
     7141 aaggtagaat tgtatttcac catctctaat tcacaattat tatctgaagc acaaaaagaa
     7201 gtgctaatcc aaaaattaca acacctgctc aacagcgaag gtgaattgcg tattgtttgt
     7261 gaagaagctc gttcacaact caaaaacaaa caattagtca tagaaaaatt atatgcactt
     7321 tttgaaaagg ctttaacacc ggtgaaaaaa cgtgttaaaa caaaaacacc aaaatcagtt
     7381 atcgctaaaa gaataaaaga taaaaaaata gccggcgaaa aaaaatcgat gcgtaaaaaa
     7441 ccggatttgg attaatcaac ttattgcttc aattctttat ttaaaattgg tgttattacc
     7501 tcagctttcg tcaacaccat catgtgtgaa cctccgggaa taatataatc tgcagtaata
     7561 ttttttattg gtaatgtatt atcattatcg ccatgaatat gaatgatatt tttaccggta
     7621 ttatcttcag gtttacgttg ccagctgaca atcatttcag tagcgcgttt aacaaatatt
     7681 ttttctttgt cccataacat ggcattacat gttgcacgtt ccaaagctct gtcctgttca
     7741 tacatgggtt gcataataaa actggaatat ttaaataccg aagcgggaat ataacgatgt
     7801 aatggatact ggtgaaaaaa ctgatacatt tcaggaattt ccgttgaatc agttgcgctg
     7861 gaaataacaa ttattttttg tggatgtaca atatcataca tttctgttgc taacatccca
     7921 cccaatgaaa caccaataat ataaaatggt gtagtggtat ctatttgtgc acttaatctt
     7981 ttggcatatg cagacatcga ttcaaatggg agaggagtaa tataatgcac atgaatagta
     8041 tctaaatgtg catcaaactg aatgttttta taaatccgat aatccgaacc ctgccccgga
     8101 attaaataca cacgagtagt ctgctgcgca gtaacaacca taccgctaat aagcaataat
     8161 aatatcaatg caaatctgca catgaaatat aaaattacct ttttatttat aaaaacgaca
     8221 ttgagtttag aaagaaaatg taattaaatc caggcgccac gaagtctcaa agtaacgcta
     8281 agttgctcaa agttgaattc ctaacccttt gtgtcccttc gaggcgctta gcgacttagt
     8341 ggtgatataa aatatcgcct cccgaaactc cccgtcttcc cgccttcccg gcaacctcac
     8401 actcaacaac ctca
//
LOCUS       DAORKW010000099         8359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_234_length_8359_cov_5.617413, whole genome shotgun sequence.
ACCESSION   DAORKW010000099 DAORKW010000000
VERSION     DAORKW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_234_length_8359_cov_5.617413"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="PLB46_05705"
     CDS             complement(<1..1352)
                     /locus_tag="PLB46_05705"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF76044.1"
                     /translation="MSITIIVIYTIAITLLFFYCIMQLSLAIHYIKFKRNLKKEETIF
                     TIPESEYPYVTVQLPVFNELYVVERLIDAIAAFDYPADKLEIQLLDDSTDESFEIARK
                     KVDYYHQRGIDIKQIRRPERKGFKAGALEYGLREAKGEYVAIFDADFVPFPDFLKKTI
                     PHFLLDDKIGVVQAKWDHLNKDYSILTKMQAFALDMHFTIEQQGRNAAGYFINFNGTA
                     GVWRKATIEDAGGWKADTLTEDLDLSYRAQLKGWKFKYVENVVAPAELPTDMNALKSQ
                     QFRWNKGGAETAKKIGLRVINAELPLRIKLHAVAHLFNTTNYIFILITAILSVPLLFI
                     KNQVIDFNYFKYASIFLMGSIAIAYAYYISTLQREKTMRKTLSVYITRFPIFLAITMG
                     MSLHNAWAVFRGWLGQQTAFVRTPKFNIVNGKDIWKNKKYTASKISPIVYLEGLFSLY
                     "
     gene            complement(1368..2060)
                     /locus_tag="PLB46_05710"
     CDS             complement(1368..2060)
                     /locus_tag="PLB46_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF76045.1"
                     /translation="MQKISVIIPVFNESGSIGHVLKDIPADLVTEVIVVNNGSTDNTA
                     EIAKTMGATVLLEQRKGYGSACLKGINYLKQKPLAEQPEIVVFIDGDYSDYPEQMREL
                     VQPVINGDAVMVIGSRARGKRIKGSMAPQQIFGNWLATTLIKLLFGARFSDLGPFRAI
                     TWATLLDLNMQDTNYGWTVEMQIKVAKKKLRFTEIAVDYRPRIGVSKITGTVKGTIMA
                     GYKIITTIFKYY"
     gene            complement(2133..3506)
                     /locus_tag="PLB46_05715"
     CDS             complement(2133..3506)
                     /locus_tag="PLB46_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRF76046.1"
                     /translation="MSQANNSLSLSNPHPANFNTKQKTGLAITGLGLLAFFMAWAGAA
                     NHQPLIFLIITLLCFFGGGILYTIGSYGHLPKGIKNNRTMFTSISSRGALAWISGVVI
                     TGFYIMLYWRDYWTELFGADLFVGLVNLFNPLSLLLRNTKADQWFVYGTLYTIAVLVM
                     GFKFIYKYRHNRYQQIRTTSVMFFQLGFAWLLPGIMKALYNYEPYLTYFWPLDYDAIF
                     PTTLSYFQTQGAFGMFFIFWGLVMAFVATPLLTYFFGKRWYCSWVCGCGGLAETAGDP
                     FRHLSDKSMKAWKIERWMIHSVLAAIILITVLLLVDNSFKNNLLGGAAKDIAKGYSFV
                     IGMVFSGVVGVGFYPIMGSRVWCRFGCPMAAYMGLIQRFKSRFRITTNGAQCISCGNC
                     STYCEMGIDVRAYAQKGQNIVRASCVGCGVCSAVCPRGVLKLENGPDNTKRTEILFTS
                     EDLKILS"
     gene            complement(3554..4891)
                     /locus_tag="PLB46_05720"
     CDS             complement(3554..4891)
                     /locus_tag="PLB46_05720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRF76047.1"
                     /translation="MHIVIIGNGITGITAARTIRKHSDHEITVVSGETDYFFSRTALM
                     YIYMGHMQFKDTQPYEPWFWTKNRINLKRGWVKTIDTQTKQLLFSNDDRLPYDKLLIA
                     SGAKPNKFGWPGQDLVGAQGLYSYQDLEELEKNTANGINRAVIVGGGLIGMEMAEMLH
                     SRHYPVTLLVRESSYWNNILPEQESQLITRHIREHGFDLRLNETLKEIKGDANGKVTS
                     VITGSGEEIACQVVGLTAGVSPNIAFLQGTEIKTDKGVVINQHMHTNIEDIYAAGDCA
                     QFQHALPDRRVIEQVWYTGKLQGETAAYNMIGYNKVYEPGFWFNSAKFLDIEYQTYGT
                     VLNTLREGETEFYWESDDHQKCVHIVYRESDHLFIGINVFGIRMRHEIFNRWLLEKQK
                     VEHIMSHLSAANFDPEFFQLFEQQIVDAWNKQHPANTIQLKSKSLLTKLIFGN"
     gene            complement(5047..5733)
                     /locus_tag="PLB46_05725"
     CDS             complement(5047..5733)
                     /locus_tag="PLB46_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HRF76048.1"
                     /translation="METDSQPFTHEQWTNLLQKHVSADGWVNYEGFIKDSTQLNNYLQ
                     QIESNYPNEKNWSREQILAYWINAYNAYTVQIVIRNYPVASIKDIKPGVAFLNSVWDI
                     KFITIEGEQLDLNNIEHNILRKMNEPRIHFAVNCASYSCPKLLNTAYEAATIDNQLNQ
                     QAIDFINDPKRNLITPEEATVSSIFNWFTGDFTNNGSLKDFLNKYSNVPIAENTSINF
                     MEYNWNLNKQ"
     gene            complement(5955..>8359)
                     /locus_tag="PLB46_05730"
     CDS             complement(5955..>8359)
                     /locus_tag="PLB46_05730"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRF76049.1"
                     /translation="SMFAQNTSLRPVAKMVADSKSSGISFKQAAPFNVQINPAHKSTT
                     STFAKDITFVKMNSTLVKDIFTKRNAALEITLPYKESEIIVDLVQVNLFADGFQVETN
                     TSNGQPVDYTPGVYYQGTIKDDPNSIVAISFFEDEIIGLIASADKGNINIGRYGDGSK
                     EDYLIYSDWDILVTPDNAGCATVDDPEYLDEFQEIMQIEGESRITSCPKIYFEGDYQL
                     YQNKGSVTAAANYITGIYNNSAIIYTNESVPTQISTIFVWTSPDDYSTASSYDALVSF
                     MSYRTSFTGDIAHLVTLDPGGLGGIAATINGLCNPYKYCYSGIDPTYNAFPTYSWTVM
                     VVTHEMGHLFGSYHTHNCGAWAGGAIDNCYTTEGGCPAGPAPVGGGTIMSYCHLTGYG
                     INLANGFGVQPGNAIRNTISAAGCITSCGGAATYCASSGLSTADEWIQTITVAGTTKT
                     SGNNSGYADFTATTVNLTPGGTAAYSLTPGYTGTVYPEYFSIWIDYNKDYDFNDAGEN
                     VYNSGAVTSTVTGSFSVAAGMTGTTRMRISMKYNASPTSCETFDYGEVEDYTVSFTPV
                     VTYCSSAGTSAASRHIDYVKFNTINRTSGSDGGYYNGVATTTSIIKGTAYTLTYSAGF
                     SGTIYQMYWRGWIDYNRDGDFSDAGEQVFQKKATTSANLTKSIVVPATASTGTARMRI
                     SAKYGGYPTACETFTNGEVEDYTVNILPALPSGETKPFSLSIFPNPGSGVFNIEFLNA
                     VTGGVTLEVFDITGNVISNEIIASENGMIVLDITNAASGMYFVKMTQTNGTTEVKQII
                     KQ"
BASE COUNT         2546 a         1652 c         1514 g         2647 t
ORIGIN      
        1 aaatagagac tgaataatcc ttcgaggtaa acaataggac ttattttaga tgccgtgtat
       61 tttttatttt tccagatatc ttttccattc acaatattaa acttaggtgt gcgcacaaat
      121 gcagtttgct gacctaacca gccacgaaaa actgcccagg cattgtgtaa gctcataccc
      181 atggtaatgg ctaaaaatat tgggaaacgg gtaatataca cgcttaaggt tttacgcatt
      241 gttttttcgc gttgtaaagt ggaaatataa taagcgtaag caatggcaat agaacccatt
      301 aaaaaaatac tggcgtattt gaaatagtta aaatctatca cctgattttt aataaacaac
      361 agcggtacac ttaatatggc tgttattaaa ataaaaatat agttagtggt attaaacaag
      421 tgcgcaacgg catgtaattt aatacgcagt ggtaattcgg cattaataac acggaggcca
      481 atttttttag ctgtttcggc tccaccttta ttccaacgga attgctgcga ttttaacgca
      541 ttcatatcgg ttggtaattc agccggagca actacgtttt caacgtactt aaatttccat
      601 ccttttaatt gtgcacgata actgaggtca aggtcttcag ttaaagtatc agccttccag
      661 ccacctgcat cttcaatggt ggctttacgc caaacacctg cagtgccgtt aaaattgata
      721 aagtaaccgg ctgcattacg accttgttgc tcaatagtaa aatgcatatc gagtgcaaaa
      781 gcctgcattt tggtcaggat gctataatct ttattgaggt gatcccattt tgcctgaaca
      841 acgccaattt tgtcgtccaa taaaaagtga gggatagttt ttttaagaaa atcaggaaaa
      901 ggtacgaagt ctgcatcaaa aatagctaca tactcgcctt ttgcttctct taaaccatat
      961 tcgagggcac cggctttgaa acctttacgt tcagggcgac gaatttgttt aatatctata
     1021 ccacgctggt gataataatc cactttttta cgcgcaattt caaaactttc gtctgttgaa
     1081 tcgtcgagta actggatttc taatttgtca gcagggtaat cgaatgcagc aatagcatcg
     1141 attaaacgtt ccacaacata caattcgttg aacaccggca attgcacggt aacataggga
     1201 tactctgatt caggaatagt aaaaattgtt tcttcctttt tcaggtttct cttgaacttg
     1261 atgtaatgga tggccagact taactgcatt atgcagtaaa agaacagcag ggtaatggct
     1321 atggtataaa ttactattat agtgattgac atgtcaatcg gttaggttca ataatattta
     1381 aaaatggtag ttataatttt ataaccagcc ataattgtgc ctttaacggt gcctgtgatt
     1441 tttgaaacgc ctattctcgg tctgtaatct acggcaattt ctgtaaatcg taatttcttt
     1501 tttgccactt taatctgcat ttcaaccgtc catccgtaat tggtatcttg catattcagg
     1561 tcgagcaggg ttgcccaggt tatggctctg aacggcccca aatcgctgaa acgtgcgcca
     1621 aacaagagtt tgataagggt tgttgccaac cagttaccaa aaatttgttg tggtgccata
     1681 gatcctttaa tgcgttttcc ccttgctctg ctgccaataa ccataacggc atcaccgttg
     1741 ataactggtt gaacaagctc tcgcatttgc tccggatagt cggaataatc accgtcaatg
     1801 aacactacaa tttcaggttg ctcagctaag ggcttttgtt tcaggtaatt tattcccttt
     1861 aaacaagctg acccataacc ttttctctgt tccaacaaca cagtggcacc cattgttttg
     1921 gcaatttcag cagtattatc ggtagaacca ttgttcacca ctattacttc tgttaccaaa
     1981 tcagcaggta tgtcttttaa aacatgtcca atcgaaccgc tttcgttaaa aaccggtatg
     2041 attacgctga ttttctgcat gttaaacttt gagctgccac ccctgaaaaa gtgcacaaaa
     2101 ttacgtcaat tgtaaatgat atttaaataa tgttaagaga gaattttcaa atcttcagaa
     2161 gtaaacaaaa tttcggtgcg ttttgtgttg tccggaccgt tttcgagttt gagtaccccg
     2221 cgagggcaaa cggcagaaca aacgccacaa ccaacacagc tggcacgcac tatattttgg
     2281 cccttttggg catatgcacg cacatcgata cccatttcac aataggtgga gcaattgcca
     2341 caggatatac actgggcgcc gtttgtagta attctaaaac gcgatttaaa tcgctgaatt
     2401 agccccatgt aagcggccat cgggcatccg aaacggcacc atactctgct gcccataatt
     2461 gggtaaaatc caaccccgac taccccgcta aacaccatac caattacgaa actatagccc
     2521 ttggcaatat ctttagcagc accgcctaat aaattatttt tgaaactatt atcaaccaat
     2581 aataatacgg ttatcaaaat tatggctgcc aaaacgctat gaatcatcca tctttcaatt
     2641 ttccaggctt tcatggattt gtcgctgagg tgacgaaacg ggtcgcctgc tgtttcggct
     2701 aagccaccac atccacatac ccagctgcaa taccatcttt taccaaaaaa gtaggtaagt
     2761 aaaggtgttg ccacaaatgc catcaccaaa ccccagaaaa tgaaaaacat tccaaatgca
     2821 ccctgtgttt ggaaataact taaagtagta gggaaaatag catcataatc taatggccag
     2881 aaataagtta aataaggctc gtaattatac aatgctttca taattccagg caacaaccag
     2941 gcaaaaccca attgaaaaaa cataacagaa gtggtacgta tttgctgata acgattatgg
     3001 cgatatttat aaataaattt aaatcccata actaatacag caatggtata taatgttcca
     3061 taaacaaacc attgatctgc ttttgtatta cgcaacaata aactcaatgg attaaataaa
     3121 tttactaatc cgacaaataa atcagcacca aataattctg tccagtaatc acgccaatac
     3181 aacatgatgt aaaatccggt aattaccaca ccactaatcc atgctaatgc tcctcgtgat
     3241 gaaatagagg taaacatggt tcggttgttt tttattccct tcgggagatg tccgtacgag
     3301 ccaattgtat acaaaatccc cccaccaaaa aaacataaga gggtgattat taaaaatata
     3361 agcggctgat gattggctgc tcctgcccac gccataaaaa acgccagcaa acccagtcct
     3421 gtaatagcca gaccggtttt ttgtttggtg ttaaaattgg ctggatgcgg attggatagt
     3481 gataaactat tatttgcctg tgacattgta gtaagttttt attaacgatg aattagtata
     3541 aaaaacttca gcgttaattg ccgaaaataa gttttgttaa caatgatttt gattttaatt
     3601 gaatagtatt tgccggatgt tgtttgttcc atgcatcaac tatttgttgt tcgaaaagtt
     3661 ggaaaaattc cgggtcgaaa ttggcagcac ttaaatgcga cataatatgt tcaacttttt
     3721 gtttttctaa caaccagcga ttaaatattt cgtgtcgcat tctgatacca aaaacattga
     3781 tgccaataaa tagatgatcg ctttcacgat acacaatatg tacacatttt tgatggtcat
     3841 cactttccca ataaaattct gtttcccctt cacgcaaagt atttaatacg gtaccataag
     3901 tttggtattc aatgtctaaa aactttgcac tattaaacca aaaacccggt tcataaactt
     3961 tgttgtaacc tatcatatta taagcagcag tttctccctg caatttaccg gtataccata
     4021 cttgttcaat tacacgtctg tcgggaaggg catgctgaaa ttgagcacag tcgccggcgg
     4081 cataaatgtc ttcaatattg gtgtgcatgt gttggttgat caccacacct ttatcagttt
     4141 taatttcggt accttgtaaa aaggcaatat tgggcgaaac accagctgta agtcccacta
     4201 cctggcaggc tatttcttca ccactacctg taattacaga agtcactttg ccgttggcat
     4261 caccttttat ttcttttagc gtttcattta acctgaggtc aaatccgtgt tcacgaatat
     4321 gtcgcgtaat tagctgcgat tcctgctcag gtaaaatatt attccaataa ctgctttccc
     4381 gcactaaaag tgtaaccgga taatggcggg aatgcaacat ttctgccatt tccattccaa
     4441 ttaaacctcc tccaacaata accgctctgt taattccatt agctgtattt ttttccagtt
     4501 cttcaaggtc ctgataacta tacaatcctt gtgcgccaac taaatcctga cctggccagc
     4561 caaatttgtt gggttttgca cctgaggcaa tcagcaattt atcgtaaggc agacggtcat
     4621 cgttgctgaa taacagctgc tttgtttgcg tatctatcgt tttaacccaa ccacgtttga
     4681 gatttatgcg gttttttgtc caaaaccagg gctcatatgg ttgtgtgtct ttaaactgca
     4741 tatgccccat gtaaatatac atgagagcag ttctgctaaa aaaataatct gtttctccgg
     4801 atacaacagt tatttcatga tccgaatgtt tgcggattgt ccgtgctgcg gttattccgg
     4861 taattccatt tccaataatt acaatatgca tagatagtgg ctgcgaaagt aaaacgaatg
     4921 aactatgaaa ataagaacaa aatatgaata gttccgactt atttcataaa aaacgttaat
     4981 tgtatttgcg ttagtttaaa attacccaat tgttttgtgc aatttttcgc tgactaagaa
     5041 aataaattat tgcttattca aattccaatt atattccata aaatttatgg aggtgttttc
     5101 ggctattggc acattactgt atttgttcag gaagtctttc aatgagccat tattagtgaa
     5161 gtcgccggtg aaccagttga aaatgctact tacagttgct tcttccgggg ttatcaggtt
     5221 gcgttttggg tcgttaatga aatcaattgc ttgttgattg agctgattgt caattgtagc
     5281 ggcttcgtaa gctgtattga gaagttttgg gcatgaataa ctggcgcagt ttacagcaaa
     5341 atgaattcgg ggttcgttca ttttgcgtaa aatattgtgt tcaatattat tgaggtcgag
     5401 ttgttcacct tcaatggtaa taaatttaat atcccaaaca gagttaagaa acgcgacacc
     5461 gggtttaata tctttaatgc tggcaacagg ataattgcgt ataacaattt gcacggtgta
     5521 ggcattatat gcgtttatcc aataggctaa tatttgttcc ctgctccaat ttttttcgtt
     5581 gggataattt gactcgattt gttgaaggta attgttgagt tgtgtgctgt ccttgataaa
     5641 tccttcataa ttaacccagc catcggcact tacatgtttt tgcaaaaggt tagtccattg
     5701 ttcatgggta aatggttgcg aatcggtttc caagccggaa attgttctgc aggagggtag
     5761 ggtgcagctg gttgataacg caataataca aatcaataaa atcggtcgca tatatgggtt
     5821 gttatggata aatatacgca aaaaactgct tttaagttta gcaaaatgat tcgatcgatt
     5881 aatataatta ttttctaaga ataaaaaaag aggctatctg aattagatag cctctttaaa
     5941 aaatttaata ttaattattg tttaataatc tgttttactt ctgttgtgcc atttgtttgt
     6001 gtcattttta caaaatacat tccactggca gcattagtaa tatctaatac tatcatgcca
     6061 ttttcacttg caataatttc attgctaatt acatttccgg taatatcaaa tacttctaat
     6121 gttacaccac cggtaacagc atttaaaaat tcgatattaa atacacctga acccggattt
     6181 gggaaaatag ataaactaaa aggttttgtt tcaccggatg gtaatgccgg taaaatattt
     6241 acagtataat cttctacttc accattggta aatgtttcgc aggcagttgg gtaacctccg
     6301 tatttggcac tgatgcgcat gcgagctgtt cctgtgcttg cagtagcagg tacaacaata
     6361 ctcttagtta aattggctga tgttgttgct ttcttctgga aaacctgttc acctgcatca
     6421 ctaaaatcac catcgcgatt gtagtcgatc caacctctcc aatacatttg ataaattgtt
     6481 ccggaaaatc ctgcactata agttaaagtg taagctgttc ctttaattat actggtggta
     6541 gttgcaacac cattgtaata accaccatca cttcctgatg tgcggttaat ggtattaaat
     6601 ttaacataat cgatatggcg tgatgctgca ctggtacctg cactgctgca ataagtaact
     6661 accggtgtaa atgatacagt ataatcttct acttcaccgt aatcaaatgt ttcgcatgaa
     6721 gttggagacg cattgtattt catactaata cgcattctgg tagttccagt catacctgct
     6781 gcaacagaaa aacttcctgt aactgtactt gtaacggctc cagaattata cacattttca
     6841 cctgcatcat taaaatcata atctttattg taatctatcc aaatagaaaa atattccgga
     6901 tacacagttc cggtataacc aggagttaaa ctataagctg ccgttccgcc tggtgttaaa
     6961 ttaacggtag tggcagtgaa atctgcatac ccactattat tacctgaagt ttttgttgtt
     7021 cctgcaacgg taattgtttg aatccattca tctgcggttg ataatccgga tgatgcgcaa
     7081 taagttgcag ccccaccaca agaagtgata caacctgccg ctgaaattgt gtttctgatt
     7141 gcattacccg gttgaacacc aaatccattt gctaaattga taccgtaacc agtaagatgg
     7201 caataactca ttatggtgcc gccgccaact ggtgccggac cagcggggca accgccttca
     7261 gttgtataac aattatcaat tgcaccaccg gcccatgcac cacaattatg tgtatgataa
     7321 ctgccaaata agtgtcccat ttcatgtgtt accaccataa cagtccagct ataagttggg
     7381 aatgcattat acgttgggtc aataccgctg taacaatatt tatagggatt acataatcca
     7441 ttaattgttg ctgcaattcc acctaaacca cccgggtcta aagtaactaa atgagcaata
     7501 tcacctgtaa aagatgtgcg ataactcata aaggaaacca acgcgtcgta agaagatgct
     7561 gttgaatagt catctggact tgtccaaaca aaaatggttg aaatttgtgt tggtacactt
     7621 tcgttagtgt aaataatagc tgaattatta taaataccgg taatgtaatt tgctgctgca
     7681 gttacacttc ctttattttg atataattga tagtcacctt caaaataaat tttaggacaa
     7741 gaggtaattc tcgactcgcc ttctatttgc attatttcct gaaattcatc taaatattca
     7801 gggtcatcta cagtagcaca gcctgcatta tcgggagtta ctaaaatgtc ccaatcagaa
     7861 taaattaaat aatcttcttt gctgccatca ccataacgac caatattgat attgccttta
     7921 tcagctgaag caattaaacc tattatttca tcttcaaaaa agctgatggc cacaattgaa
     7981 tttgggtcat ctttaatggt gccttgataa tatacaccag gcgtatagtc aacaggttgt
     8041 ccattacttg tattagtttc aacctgaaat ccatcagcaa ataaatttac ctgaacaagg
     8101 tctacaataa tttcactttc tttataaggt aaagtaattt caagtgcggc atttcttttg
     8161 gtaaaaatat cttttactag tgttgaattc atttttacaa aagtgatgtc ttttgcaaaa
     8221 gttgaagttg tgcttttatg tgcgggattt atttgcacat taaatggagc cgcttgctta
     8281 aaggagatgc cagaactttt gctgtcggca accatttttg caactggacg tagtgatgtg
     8341 ttttgagcaa acatgctca
//
LOCUS       DAORKW010000100         8332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_235_length_8332_cov_4.707261, whole genome shotgun sequence.
ACCESSION   DAORKW010000100 DAORKW010000000
VERSION     DAORKW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_235_length_8332_cov_4.707261"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PLB46_05735"
     CDS             <1..424
                     /locus_tag="PLB46_05735"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRF76050.1"
                     /translation="LKKIVMLISKVSYLGDLRTSATHLGSGTTIITDAPLDNQGKGEA
                     FSPTDLVATATASCMLTLMGIAARTHGLNIDGTTVEVTKVIASNPRRIAEIHCKLVIP
                     AGTLTAKDKQILKIAGKTCPVIQSLSTEIIKVLTFEYR"
     gene            complement(484..633)
                     /locus_tag="PLB46_05740"
     CDS             complement(484..633)
                     /locus_tag="PLB46_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76051.1"
                     /translation="MFKARSITRVFIAAGLAVLLTVSISSCGHKEGCPNKITEVAGVK
                     ITTTT"
     gene            787..2127
                     /locus_tag="PLB46_05745"
     CDS             787..2127
                     /locus_tag="PLB46_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRF76052.1"
                     /translation="MQIHLIAIGGAVMHNLALALHQIGYKVTGSDDEIFEPSLSRLKQ
                     HGLLPAEKGWFPDKIHDKLDAVILGMHARADNPELLKAKDLGLAVYSFPEYVYEQSKD
                     KIRVAICGSHGKTTITSMVMHVLKQQNMAFDYLVGAQLAGFETMVQFSDAPLMIIEGD
                     EYFASPLDKRPKFLHYQSQITLISGIAWDHFNVFPTFGNYTDQFKQLMEGTKSTDSLI
                     VFNDDINIQSLINESKIAAKIIPYETPAYVLNNHTFQIITDSNQYTFSIFGRHNMQNM
                     EGAKKVCNALGISDADFYTAMQSFKGAAKRLEILKQNNDHIIFRDFAHAPSKVEATVN
                     AVKEQYPEKILVACLELHTFSSLNKDFINQYHNTMQKADVRIVYFNPHTLVIKKLPSL
                     STLEVQHYFNDNDMYVVNDSAQLLELLQKLNIVNTNLLLMSSGNFDNLDLTILT"
     gene            2142..2909
                     /locus_tag="PLB46_05750"
     CDS             2142..2909
                     /locus_tag="PLB46_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRF76053.1"
                     /translation="MLKLDKPLAFLDLETTGINLGADRIVEISILKISPDGKEESLTM
                     RINPGIAIPKESTAIHGISDEDVKDAPLFKDAAEQLNAFLHNCDIAGYNSNKFDIPML
                     AEEFLRADKGFDENRRYIDVMRIFTLMEKRTLEAAYKFFCNKELTNAHSAEADVRATY
                     EVLLGQLDRYKDQLKNDVQSLHEFTKDGDFVDFGRRMIMKDGKAYFNFGKYTGQACVD
                     VYKREPQYFDWIYKSDFPLHTKLKLKMIEFKIKTQNT"
     gene            2915..3646
                     /locus_tag="PLB46_05755"
     CDS             2915..3646
                     /locus_tag="PLB46_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRF76054.1"
                     /translation="MHGTIVVIPTYNEQANIVAMIAAVLDTPDNFHLLIIDDNSPDGT
                     ALDVEKLQTKYPNRLFLHKRSGKLGLGTAYITGFKWALDHGYDFIIEMDADFSHKPSD
                     LPRLKATCEAGADVAIGSRYVKSGRVEDWPLNRILLSKGASLYVRLITGIPVKDTTAG
                     FVCYKRAVLEKIDLDKIKFIGYAFQIELKFAAWKCKFKLKEIPIVFKDREKGVSKMSK
                     GIFKEAVLGVIKMKWNSFSDSYVKH"
     gene            3651..4736
                     /gene="corA"
                     /locus_tag="PLB46_05760"
     CDS             3651..4736
                     /gene="corA"
                     /locus_tag="PLB46_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRF76055.1"
                     /translation="MSAPLTHRSKKHTHKRKSGLLPGTAIYTGNKHPENISISRIDYS
                     EQHFSEKSFTQGDNIPMDVVDNNIMWLNVNGLNIEALIEKIGIHFKLHNLIVEDILNV
                     YQIPKADEYPESDIIYITLNEFYFDDAQRLQRDQISLILGGDFVLSFQEEEGDYFGII
                     RDRLRNAKGKIRKKCADYLIYVLIDAIVDSYYDILDHYANELEAIENDIFIQKNKNHL
                     SRIHHVNKDLIYLRRSIAPLNEVIFRLMKEEIVLIQPETKIFLRDVLDHVNQVVNQID
                     VDREYLSDLVQTNMANMNSHLNSIIKVLTLISTIFIPLTFIVGVYGMNFDNFPEIHSQ
                     NGYYIVWGVMIAITILQLLIFKKKKWL"
     gene            4763..5263
                     /locus_tag="PLB46_05765"
     CDS             4763..5263
                     /locus_tag="PLB46_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76056.1"
                     /translation="MKKLSLFLFITLITGSLIAQTTVANKKSATYFGANFDLGFPFGS
                     FQTINDEIAIGGGVNLFFQPDKKIPILLGFDIAFLGNGHKSQTETLTATIEANGTIIQ
                     TLSFPLRAETSNIITKGHLNVRFLAPTKTFQPYVDGLIGFNHFGTTTAIYDESPEHFL
                     PKKITI"
     gene            5374..5553
                     /locus_tag="PLB46_05770"
     CDS             5374..5553
                     /locus_tag="PLB46_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76057.1"
                     /translation="MGSKAEYYVEDDIEQWEISYNSSILSEEEIDEDDISIAATPKES
                     RTDMMVGTVGISFKF"
     gene            5752..6285
                     /locus_tag="PLB46_05775"
     CDS             5752..6285
                     /locus_tag="PLB46_05775"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRF76058.1"
                     /translation="MMLRFIAPGTKTKLPGMPIDSLNHIPVYYNGDIHHVGKEITTST
                     GYVLGVEWQCVEFVKRYYYLRFNHIMPNGSGNAKDFFDKQLSDASYNYARELVQYVNP
                     SLKKPAVEDIIVFDGNLLNNYGHIAIISAVNEHTIEIIQQNGGPEAPSRLLLDLVYEN
                     GRWEVKHKRILGWLRIE"
     gene            complement(6389..6898)
                     /locus_tag="PLB46_05780"
     CDS             complement(6389..6898)
                     /locus_tag="PLB46_05780"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HRF76059.1"
                     /translation="MKNFRLILTLLTIVLLSQSTFAATPDVPTPDEMSSSKINLWIPG
                     FLMKMAAEIADDHVDDIEDEQAIDLLRKFGNTTICIREGEYYKDRTDKKMTRKLDRME
                     RKNYSELMSVITAEEQVNISIKENKKGKIKRMVVLVDEKDNTYVFLKMNCKLSIDDIS
                     KVCSQFTED"
     gene            complement(6938..7459)
                     /locus_tag="PLB46_05785"
     CDS             complement(6938..7459)
                     /locus_tag="PLB46_05785"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HRF76060.1"
                     /translation="MKKLILSAILFAAATSLFAQNAQIEQFYQKYEGKEGFTSVKITE
                     KLFALAASATGSDPDIQNIVDGIKGIQILVFENTENNAKSGEYYREFMSTVSTSNFDE
                     LMTVNSEGDKVKFYGKMASEKVLNEMLLVCDSDGEFVMISILGEINMEDISKLSEMDI
                     NGLEELKKVEDKE"
     gene            complement(7581..8054)
                     /locus_tag="PLB46_05790"
     CDS             complement(7581..8054)
                     /locus_tag="PLB46_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76061.1"
                     /translation="MPDYIQTGLSTFKDLFTWLSDRSKSKDITKNAILREIRDNLKML
                     EHRNQEGVNITALIAGLSGNAIAEAYVNNYKFNALKDGPKQLSKQLIFNKRQEKYIGW
                     DAERFVYSIEGKIKDLKNIPVLFADLSKAPVNITVRLDNLYYQLLLFAIFLHNKQ"
     gene            complement(8091..>8332)
                     /locus_tag="PLB46_05795"
     CDS             complement(8091..>8332)
                     /locus_tag="PLB46_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRF76062.1"
                     /translation="TPTPYVEAQPGDLILFAGPNNGPGNPGHVGIVLSFEPETGFSFI
                     HTSSPESGGVRISNEKSEKYYNKKFLEIRRVITTD"
BASE COUNT         2746 a         1388 c         1506 g         2692 t
ORIGIN      
        1 tttaaaaaaa attgtcatgt taatatcaaa agttagttat ttgggggatt taagaacttc
       61 ggccacacat ttaggttcgg ggactactat aattaccgat gcaccactgg ataatcaagg
      121 gaaaggagaa gcattttcac cgacggattt ggttgcaact gctacagcga gttgcatgct
      181 tacgctgatg ggcattgcag cgagaactca cggtttgaat attgacggca caaccgttga
      241 agtaaccaag gttatagcat caaatccaag gcgtattgcc gaaattcatt gtaaactggt
      301 aattccggcc ggaaccctta ccgctaaaga caaacagata ttgaaaattg cgggtaaaac
      361 ctgccctgta attcagagtt tgagcactga aataatcaaa gtgctcacat tcgaatatcg
      421 ttaaaataaa aagcccgatc ttaattaaaa gatcaggcaa catatgtttg cggttaccaa
      481 aaatcaggta gtagtggtaa tttttacccc tgctacttct gtgattttgt ttggacaacc
      541 ttctttatgg ccgcaagaag aaatagaaac ggttaataat acagctaatc cggctgcaat
      601 aaaaactctg gtgattgaac gtgctttaaa cataatactg aatatttctt ttgaacaaat
      661 taactgtgtg tgcagttcat ttgatgttta actgtattaa taacgtaaag atgcaaaata
      721 tattttaact ccgcaatcca cactcccact ttgtcaacaa cagccgtatt gttttatctt
      781 cggcgcatgc aaattcatct aatagcaatt ggtggggcgg tgatgcacaa tctcgcgctt
      841 gccctgcatc aaataggtta taaagttaca ggatcagatg atgagatttt tgaaccatct
      901 ctgtcgcgac ttaagcaaca tgggctttta ccggcagaaa aagggtggtt tcctgataaa
      961 attcacgata aattagatgc tgttattttg ggaatgcatg cccgggcaga taatcctgaa
     1021 ctattaaagg ccaaggattt agggctcgcg gtatatagtt ttccggaata cgtgtacgag
     1081 caatcaaaag ataaaatcag agtggcaatt tgcggaagtc atggtaaaac aaccatcact
     1141 tccatggtga tgcatgtact taaacaacaa aacatggcat ttgactacct ggtaggtgcg
     1201 caactagcag gatttgagac aatggtgcaa tttagtgatg ctccgctgat gattattgaa
     1261 ggagatgaat attttgcttc accattagat aaacgaccta aatttttaca ttatcaatct
     1321 caaataactt taataagcgg aattgcgtgg gatcatttta atgtatttcc aacatttggt
     1381 aattataccg atcagtttaa acaattaatg gaaggcacaa aatcaacaga ttcattaatt
     1441 gtatttaatg atgacatcaa tattcaatcg ttaattaatg aaagtaaaat tgctgccaaa
     1501 ataattccat acgaaacacc tgcgtatgtt ttaaataatc acacatttca aattattaca
     1561 gattctaatc aatatacatt ttccattttc ggcagacata atatgcaaaa catggaaggt
     1621 gctaaaaagg tctgtaatgc tttaggaata agtgatgcag acttttacac tgccatgcaa
     1681 tcatttaaag gtgctgctaa acgtctcgaa attttaaaac aaaataatga tcatattatt
     1741 tttagagact ttgcacatgc accgtcaaaa gttgaagcaa cagttaatgc tgtgaaagaa
     1801 caatatcctg aaaaaatatt agtggcatgt ttagaattac atacgttcag tagtttgaat
     1861 aaagatttta taaaccaata tcataatacc atgcagaagg cagatgtacg tattgtgtat
     1921 tttaatccgc atactttagt tattaaaaaa ttaccttcac ttagtacttt agaagtgcaa
     1981 cattatttta acgataatga tatgtatgta gttaacgatt cagctcagct attggaactg
     2041 cttcaaaagc taaatattgt taatactaac ctgttattaa tgagttcggg caactttgat
     2101 aacctcgatc ttacaatttt aacataattt tgagcgtaac tatgttaaaa cttgataaac
     2161 cactggcttt tctagacctt gaaacaacag ggattaacct tggtgccgat agaattgtag
     2221 aaatcagcat actgaaaatt tcaccggatg ggaaggaaga aagccttacc atgcgcatta
     2281 atccgggtat cgcaattccg aaggaatcca ccgcaattca tggtattagt gatgaagatg
     2341 ttaaagatgc gccattattt aaagatgcag cagagcagct aaatgcattt ttgcacaatt
     2401 gtgatattgc cggatataat tccaacaaat ttgatattcc aatgcttgct gaggaatttt
     2461 tacgagcaga caagggattt gatgaaaaca gacggtacat tgatgtgatg cggattttca
     2521 cccttatgga aaaacgaaca cttgaagctg cttataaatt tttctgcaat aaggaactca
     2581 caaatgcgca tagtgcagaa gcagatgtga gagctactta tgaagtttta ttaggtcaat
     2641 tagaccgtta taaagaccaa ttgaaaaatg atgttcaatc attacatgag tttacaaaag
     2701 atggggattt cgtggatttt ggcagaagaa tgatcatgaa agacggcaaa gcgtatttta
     2761 attttggaaa atatacagga caggcgtgtg tcgacgttta caaacgagaa ccacaatatt
     2821 ttgactggat ttataaaagt gatttcccat tacacactaa attgaaatta aaaatgattg
     2881 aattcaaaat taaaactcaa aatacctaat ccctttgcac ggaacaatag ttgtaatacc
     2941 aacctataat gagcaggcaa atattgttgc catgattgct gctgtactag acactcctga
     3001 caattttcat ttgctcatta ttgatgataa ttctccggat ggaactgcat tggacgttga
     3061 aaaactgcag accaagtacc ccaatcgatt atttttacat aaacgaagtg gaaaactcgg
     3121 attaggcact gcttatataa caggatttaa atgggcactc gatcacggtt atgattttat
     3181 tatcgaaatg gatgctgatt tttcgcataa accttccgac cttccacgtt taaaagcaac
     3241 atgtgaagct ggtgcagatg tagccatagg ttctagatat gtaaaatcag gtcgggttga
     3301 agattggcca ttaaaccgaa tattattatc taaaggtgct tctttgtatg tgcggctgat
     3361 aaccggcatt cctgtaaagg acactactgc cggttttgtt tgttataaac gcgctgtttt
     3421 agaaaaaatt gatttggata aaattaaatt tattggctat gcatttcaaa ttgaattaaa
     3481 atttgctgca tggaagtgta agtttaaatt aaaagaaatt ccaatcgtgt ttaaggatag
     3541 ggaaaaaggc gtatccaaaa tgagtaaagg tatatttaaa gaagctgtgt tgggtgttat
     3601 caaaatgaaa tggaatagtt tttcggatag ttatgttaaa cattaaaaaa atgagtgctc
     3661 cacttacaca tcgttcgaaa aaacataccc ataaacgcaa gtccggcctt ttacccggaa
     3721 ctgccatcta caccggcaac aaacatcccg aaaacatttc tataagtcgc atcgattata
     3781 gcgagcagca tttttccgaa aaatcattta ctcaaggcga taatattccc atggatgttg
     3841 ttgataacaa catcatgtgg ttaaatgtta acggcttaaa tattgaagcg cttattgaaa
     3901 aaattggtat acactttaaa ttacataatt taattgttga agatatttta aatgtatatc
     3961 agattcctaa ggccgatgag tatcctgaat cagatatcat ttatattact ttaaatgaat
     4021 tttatttcga tgatgcacaa cgtttacaac gcgaccaaat cagtttaatt ttaggtggtg
     4081 attttgtact tagttttcag gaagaggaag gtgattattt tggcattatt cgcgatagat
     4141 taagaaatgc gaaagggaaa atcaggaaaa agtgcgctga ctatttaatt tatgtgctta
     4201 ttgacgcaat agttgatagt tattacgata ttctggatca ttatgctaat gaattggagg
     4261 caattgaaaa tgacattttt attcaaaaaa ataaaaatca cctgtctcgt atccatcatg
     4321 tgaataaaga cttaatctat ttaagacgtt ctattgcacc gctaaatgaa gtgattttca
     4381 ggttaatgaa ggaagaaatt gttttgatcc agcctgagac aaaaatattt ttgcgtgatg
     4441 tacttgacca tgtcaaccag gttgttaatc aaattgatgt agacagggag tatttaagcg
     4501 acctggtaca aacaaatatg gccaatatga atagccattt gaactccatt atcaaagttt
     4561 taacacttat atctaccatt tttattccgt tgacatttat agtcggggtt tatggtatga
     4621 actttgataa ctttcctgag attcactctc aaaacggata ctatattgtt tggggggtga
     4681 tgatagctat tactatactc caacttctta ttttcaagaa gaagaagtgg ttgtaaaatt
     4741 cattcaccat aaaaattttg ttatgaaaaa actttcactc tttttattta ttacattaat
     4801 aacagggagt ttgattgctc aaaccactgt tgccaataaa aaatccgcaa cctattttgg
     4861 tgcaaatttc gatttaggat ttccatttgg ttcgtttcaa acgattaatg atgaaattgc
     4921 cattggaggt ggtgttaatt tattttttca acctgataaa aagatcccta ttttattagg
     4981 atttgatatt gcatttttgg gcaatggaca caaatcacaa accgaaacat taactgcaac
     5041 tattgaggct aacgggacaa taattcaaac cttaagtttt cccttacgtg ccgaaaccag
     5101 caatattatt acaaaagggc atttaaatgt tcggttttta gcgccaacaa aaacatttca
     5161 accttatgta gacgggttaa ttgggtttaa tcattttgga acaactacag ctatttatga
     5221 tgaatctccg gaacattttt taccgaagaa gataacgatt taattacctc aagttcacaa
     5281 aattcaagct gggcagttag ttatgggggt gctgcaggtg taatggttga aattaaagaa
     5341 aatatatttt tagacttacg gtttttatat acaatgggtt cgaaagctga atactatgtg
     5401 gaagacgaca ttgaacaatg ggaaatatct tataactcat ctattttaag tgaagaggaa
     5461 attgatgaag acgatatttc aatagctgca acaccaaagg aatcgcgtac tgatatgatg
     5521 gtaggcactg taggaataag tttcaaattc taactgtttt gcaacatatt tcgagggact
     5581 gttttagagc agtccctttt tttattgata taaatatgac agaaaaacca tacaaatttt
     5641 atctttagca tcgttatcag caacctgatg tttacgcata aacacccttt aaccaaaaaa
     5701 ttattaatca tataccttct ggtagttggt gggtttttgt caattacatt tatgatgctt
     5761 cggtttattg caccgggtac taaaactaaa ctacctggaa tgccaatcga ttcgcttaac
     5821 catattccgg tttattataa tggagatata catcatgttg gaaaagaaat aaccacatca
     5881 acaggttatg tattaggcgt agaatggcaa tgtgtagaat ttgtaaagcg ttactattat
     5941 ttacgtttta atcatattat gccaaacggt tcaggtaatg cgaaagactt ttttgacaaa
     6001 caattaagtg atgcatctta taattatgct cgagaattgg ttcaatatgt caatcccagt
     6061 ttaaaaaaac cggctgtgga agatatcata gtatttgatg gaaatttatt aaataattac
     6121 ggccatatag ccataatttc tgcagtaaat gaacatacga ttgaaattat ccaacaaaat
     6181 ggagggccgg aagcgccgag cagattacta cttgatttag tttatgagaa cggtcgctgg
     6241 gaagtaaaac ataaacggat acttggctgg ttgcgtattg agtaattccc aaaaaaaagc
     6301 cgcagacact cctgactgcg gcttgagaag catttgcttc ggtctatgaa ctaatttttg
     6361 cgaaaaatta ttcttctatt tttattcatt aatcctcagt aaattgactg cacactttac
     6421 taatgtcgtc gattgataat ttacaattca ttttgagaaa tacataagta ttatcctttt
     6481 catctacaag caccaccatt cttttaattt tacccttttt attttccttg atactgatat
     6541 tcacctgttc ctctgcagtt atcacactca tcaattcgct ataattttta cgttccatgc
     6601 gatccaattt acgagtcatt tttttatcgg ttctatcttt ataatattca ccttctcgta
     6661 tacaaatggt agtatttcca aatttgcgca gtaaatcaat agcttgttca tcctctatat
     6721 catctacatg gtcatcagca atttcagctg ccattttcat aagaaacccc ggtatccata
     6781 agttaatttt tgaagaactc atttcatcag gtgtaggaac gtcaggcgta gcagcaaaag
     6841 tgctttggct taataacacg atggttaata gcgttaatat caggcgaaaa tttttcataa
     6901 ttatcaagtt taagtttggg ttcaattgtt atcagattta ctctttgtct tcaacttttt
     6961 tcagttcttc taatccgtta atatccattt cagacaattt tgaaatatct tccatgttaa
     7021 tttcaccgag tatacttatc attacaaatt cgccatcaga atcacataca agtaacattt
     7081 catttaatac tttttctgaa gccattttgc cgtagaattt cactttatcc ccttctgaat
     7141 ttacggtcat taattcatca aaattagaag tagaaaccgt tgacataaat tcgcggtaat
     7201 attcaccgga tttggcattg ttttcagtat tttcaaaaac caaaatctga atacctttaa
     7261 tgccgtcaac tatattttga atatcaggat cagagccagt tgcagaagct gcaagtgcaa
     7321 ataatttttc agtaattttt acagaagtaa atccttcttt accttcatat ttttgataaa
     7381 attgttctat ttgagcattt tgagcaaaaa gagatgttgc agctgcaaat aaaattgcgg
     7441 atagaattaa ttttttcatg ctatagtttt ttatggttaa aaattgcttg ttaccataag
     7501 tcgaaccaag tatgaaaaag ttacagggca gtcgaaaaaa aatgcagtat cctcaaaatt
     7561 taacatttga ggtagtgtaa ttattgctta ttatgcaaaa atatagcgaa cagcaacagt
     7621 tgataatata ggttgtctaa acgtactgtg atattaaccg gtgctttact aaggtcagca
     7681 aataatacgg gaatattttt caaatccttt atttttcctt caatactata cacaaaccgt
     7741 tcagcatccc aacctatata tttttcctga cgtttgttaa agatgagttg ctttgatagt
     7801 tgtttggggc cgtccttaag cgcattgaat ttatagttat ttacataagc ttcagcaatg
     7861 gcatttccgc ttaaaccagc tataagcgcg gttatattaa ccccttcctg attcctgtgt
     7921 tccaacattt tcaaattatc gcggatttca cgaaggatgg cgtttttagt aatatctttt
     7981 gacttagacc tatcgctgag ccaggtaaat aagtctttaa aagttgagag tccggtctgg
     8041 atatagtcag gcattgaaaa aattaacaac gcaaaataaa caatatctga ttaatcggtt
     8101 gtaataaccc tccttatttc aaggaatttt ttgttgtagt atttttcgct tttttcgttg
     8161 ctgatgcgca ctccgccgga ttcaggagaa gaagtatgta taaatgaaaa tccggtttcg
     8221 ggttcaaagg aaagtacaat tccgacatga ccggggttac cggggccatt attggggcct
     8281 gcaaacagaa ttaagtcgcc cggttgggct tctacataag gagttggtgt tc
//
LOCUS       DAORKW010000101         8275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_238_length_8275_cov_6.617032, whole genome shotgun sequence.
ACCESSION   DAORKW010000101 DAORKW010000000
VERSION     DAORKW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_238_length_8275_cov_6.617032"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..2212)
                     /locus_tag="PLB46_05800"
     CDS             complement(314..2212)
                     /locus_tag="PLB46_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRF76063.1"
                     /translation="MSDQIKHECGIALIRLLKPLSYYHKKYGTPLYGLNKLYLLMEKE
                     HNRGQDGAGIATIKIDSAPGYKFLDRKRSVAPSAIKDIFEDVFKKARKIERKNPELYN
                     DTDWMKKNVPFTGEVLMGHLRYGTFGGNDLDSVHPFVRENNWITRNLIVAGNFNMVNN
                     EFLFQQLLELGQHPKQFTDTVTVMEKIGHFLDDEVQRLFDERKEYHDNKTLTHLIASD
                     LDIQRILTRASKDFEGGFAMCGMFGHGDAFVLRDPNGIRPLYFYQDDEVVVAASERPA
                     IMTTFNVPFEKVNELKPGHALIVKKKGAVKEAMVIPPKEKLSCSFERIYFSRGNDKEI
                     YEERKNLGRFLTERVLQSVNYDFDNTIFSFIPNTAETAFYGMVKGIEEYLNNYKSEEI
                     KKLNGKLDTAELQRILNLRPRVEKLALKDVKLRTFITNDSDRGDLVSHVYDVTYGQVN
                     DGKDTVVLIDDSIVRGTTLKDSIIAILDRLNPKKIVIVSSAPQIRYPDCYGIDMSKMG
                     DFVAFRAMIELLKENKKEHLIQEVYDECVAVTNMSNDKSYNAVKKLYDQFTDDQISKK
                     IAEIVKAKHIRAEVEVIYQSVDSLHLACPKNLGDWYFTGNYPTPGGNKVANKAFMNWV
                     EKKDVRAY"
     gene            complement(2380..3345)
                     /locus_tag="PLB46_05805"
     CDS             complement(2380..3345)
                     /locus_tag="PLB46_05805"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF76064.1"
                     /translation="MKEFKNDVEAIDALSADFKRLKAEIGKVIIGQDEVVQNVLMSIF
                     ADGHSLLVGVPGLAKTLLIKTISDALHLSFKRIQFTPDLMPSDITGSEILDDNRNFKF
                     LKGPVFANIILADEINRTPPKTQAALLEAMQEKNVTIGGHLHKLDLPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFNIILDYPSFEAELNIVKSTTTDTKATLSKIIGGEEIIAYQH
                     LIRRIPITDNVLNYAVSLVAKTRPNTEKADAKATQYLSWGAGPRASQYLVLGAKCHAA
                     INGKYAPDIEDVKAVALPVLRHRIVRNYKAEADGIGVDDLIKGLL"
     gene            complement(3412..4764)
                     /locus_tag="PLB46_05810"
     CDS             complement(3412..4764)
                     /locus_tag="PLB46_05810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF76065.1"
                     /translation="MKKPIITCLLVFISAIAFAQPTANKPIVDKIVGQVGDKIILMSE
                     IESFYFEQLQKGEVPEDYRCQVMQELVTQKLMLIQAAKDSIEVTDDEVESNIENRIRY
                     FISLFGSQEKMEEFYGKSVQEIKEEFRDDVRNLLLIQRMQDNIFGEMSVSPTEVKAFY
                     NAIPKDSLPLINAEIEYAQLLIIPQANAEQKAIAKQKAEDIRKRIIAGEDFCSLASIY
                     SGDGSKDDCGFLGCEPREKYVTEFSAAAFKLQPGEISEIVETQFGYHIIKMDSRQGDK
                     ACLRHILIMPPVTNLNFVAANKQLDSIRANIVAGKLTFCEATKKYSDDENSNKTCGTV
                     VNPQTGESTFEIAELSPDDYYSIENLKPGEISQVLPFTTQDGKKAIRLIKLIDQSAPH
                     SLNLKDDYAKIQNAAMSQKQVAVMRAWVEEKAAKEYIKVDPAYNNCGNLDILLNTVSK
                     "
     gene            complement(4761..5621)
                     /locus_tag="PLB46_05815"
     CDS             complement(4761..5621)
                     /locus_tag="PLB46_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76066.1"
                     /translation="MSFKNVYIALLLSTVVLGCNRHKPEKDQEPLARVYDKYLYVTDI
                     QGLINDKTSPEDSARIVSEYVENWVRQNLILRVAEDNLQSALANINKQAEAYKESLVI
                     YAYERQWLAENLDTVVVDDSIRSYFDNNQKDFTLKTDIYQLAYAVVPAAVKSADSIQY
                     WFSRGIEKYRLPIERYCAANCESFSLNSDIWLNEDDLFKLLPYDMYAGGKFRTKGIVT
                     YRDTSFRYFVKVEDFHLAGDLGPFEYQKKGIKEIIINKRKMALLDKTYKDIYTEGLKR
                     NNAEYINKEQ"
     gene            complement(5628..7589)
                     /locus_tag="PLB46_05820"
     CDS             complement(5628..7589)
                     /locus_tag="PLB46_05820"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF76067.1"
                     /translation="MKMNIFKTLMLLAGLLLITSVNAQSTGGNEVLFTVAGQPVTKEE
                     FLYVYKKNNPDKQNDYSKESLDEYLDLYINFKLKVAEARAERIDTTEKVREELQKYGD
                     QLIKSNFDKEVLEAAALKMYNRQQSERLVYHIMIAIDAAAPGADTTGAINKLKAAAER
                     LKKGEDFGKVAAEVSTDKTNATDPGLIGWITAGQIPDANFENAAFETPVGTTSNIVKS
                     KFGYHIIKVAKERPSQGTVTVAHILIKTGKEAKPEDIAKAKVKIDSIYNLVKSNPATF
                     EELAGRYSEDKVSAANGGKLEPFGTGKMVLPFQEAAFALKNPGDIAAPVQTTFGYHII
                     KLIEKKPNGTFAEVKDELKGKIERSPEYKTLRSDFVEKVKTKYPFTENSEAKKEMLAK
                     LDSSFVKNTWLMSQAANMNKTMFSIGNRNFTQSDMASYLEINQRVSRDKNIEEKYNKL
                     YTQALEQTLIEYDLSERNIDFKRLLQEYRDGLPLFAMLERKIWSKGSTDTIGLKNYYE
                     AHKNEYMWEERIDATIYTCADVKTAKAVRKLAGKNQSDKAIMDKFNVDSTELVSIKSA
                     LFLPGQDSNVDLLNKTVGIGSDILNANGSITFVKINKIVAPAPKTLDEARGYVISAYQ
                     DQLEKQWIEELHKKYPVSVNEKVFNSMVR"
     gene            7803..>8275
                     /locus_tag="PLB46_05825"
     CDS             7803..>8275
                     /locus_tag="PLB46_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76068.1"
                     /translation="MSISKNKVVSLTYELREDNVSGDLIEVVKADQPFVFLFGAGGLL
                     PEFESNLEGKIIGSNFEFQILSANAYGEFDETAMEYVPKDIFMIDGQLADDLLEVDKV
                     INLRDQGGHLVRARVAEVGEAEVLLDFNHPLAGVNLHFKGEILDIREASQEEIEHG"
BASE COUNT         2406 a         1628 c         1433 g         2808 t
ORIGIN      
        1 aaatttgtca gagtccctaa aaccttccag gtttaaacct ggaaggtttt aagacctgga
       61 aggtgtatgt taaaatacaa atctcaagcg gttaagtttt tgttgcgatt aaatttcgaa
      121 tattaaactc agaatttaac acgtaaaaaa aaattaaaaa taggggctgc aaatttgtca
      181 ggcgcgattt tttagatgca tgtaaaacaa tatattgcat gcaattaaaa tctatttgtt
      241 ttacaataaa aaataaagct tttagttaat tatacctgca actgtagaat agttactata
      301 aaaacttttt atttcaatac gcccttacat ccttcttctc tacccaattc ataaatgcct
      361 tgttcgcaac tttgtttcca cccggcgttg gataattgcc ggtgaaatac caatcgccta
      421 aatttttagg gcaggctaaa tgcaaactgt ctaccgactg ataaattact tctacctctg
      481 cgcgaatatg cttcgctttt acgatttcag caattttttt ggagatttga tcatcggtaa
      541 actggtcgta taattttttt acagcgttat aagatttatc gttgctcata ttggtaacag
      601 caacacattc atcatatact tcctgaatta aatgttcttt tttgttttct ttcaacaact
      661 caatcattgc tctgaacgca acaaaatcgc ccatcttact catgtcgatt ccataacaat
      721 ccggataacg aatttgtggt gccgaagaaa ctatcacaat ttttttcgga tttaatctgt
      781 ctagaatggc aataatacta tcctttaatg tagtgccacg aacaatactg tcgtcgatta
      841 atactactgt atcttttcca tcattaactt gtccgtacgt tacatcatac acgtgactca
      901 ctaaatcacc acggtcagaa tcgttggtga taaatgtgcg gagttttaca tcttttaatg
      961 caagtttctc aacgcgcgga cgtaaattta aaatacgttg caactcagca gtatctaatt
     1021 taccattgag ttttttaatt tcctccgact tataattatt taaatattct tcaatacctt
     1081 ttaccatacc ataaaatgcg gtttcagcag tattgggaat aaatgaaaat atggtattat
     1141 cgaaatcgta attaacagac tgtaaaactc tttccgttaa aaagcggccg agattttttc
     1201 tttcttcgta aatttcctta tcgttaccac gtgaaaaata aatgcgctcg aaactgcagg
     1261 ataatttttc cttcggagga attaccatgg cttctttaac agcacctttt ttcttaacaa
     1321 ttaatgcatg ccccggtttt aattcattta ctttttcgaa aggcacatta aacgtagtca
     1381 taattgccgg gcgttcactt gccgcaacaa ctacttcatc gtcctggtaa aaatataatg
     1441 gacgaatgcc attcgggtcg cgcaaaacga atgcatcgcc gtgaccaaac atgccgcaca
     1501 tggcaaatcc gccttcaaaa tcttttgatg cgcgggttaa aatgcgctga atatctaaat
     1561 cgctggcaat taaatgggtt aatgttttat tgtcgtgata ttcttttctt tcatcaaata
     1621 aacgttgcac ctcatcgtcc aaaaaatgcc cgattttttc catcacggtc accgtatcgg
     1681 taaactgttt agggtgctga ccaagttcta ataattgctg gaacaaaaat tcattgttca
     1741 ccatattaaa attaccggca acgataaggt tgcgggtaat ccagttattt tctcttacaa
     1801 atgggtgaac actatctaaa tcgttgccgc caaaagtacc atatcgcaaa tgtcccatca
     1861 aaacctcacc ggtaaacgga acattttttt tcatccaatc ggtatcgtta tataattccg
     1921 ggtttttgcg ttcaattttt ctggcctttt tgaaaacatc ttcaaaaatg tctttaatag
     1981 ccgaaggggc tacactacgt ttgcggtcta aaaatttgta accgggagcc gaatcaattt
     2041 taatagtagc aatacctgca ccgtcctgtc ccctgttgtg ttccttttcc atcaacaggt
     2101 agagtttatt taatccatac aaaggagtgc catatttttt atggtagtag gaaagtggct
     2161 ttaataatcg aatgagggct atgccgcatt catgcttgat ttgatcgctc acaataaatg
     2221 tttcagaaaa ttgaaccgca aatttagtca attagcagct taaatattca ataaaaacac
     2281 caatagcagg gataagttcc cgttaacgct tgtacaacac caatttgcca tgtttacaac
     2341 atggttaact gtgcagattt acatctaaat tgggctaatt tataaaaggc ctttaatcaa
     2401 atcgtcaacc ccaataccat cggcttcggc tttgtagttg cgcactattc ggtgacggag
     2461 cacagggagc gctacagcct taacatcttc aatatcaggt gcatatttac cattaatagc
     2521 agcatgacat tttgcaccaa gcactaaata ctgtgatgct cgaggccctg caccccaact
     2581 gaggtattgt gtagccttgg catctgcttt ttcagtattc gggcgtgttt tcgctactaa
     2641 tgatactgcg taattcaaga cgttgtcggt aattggaatg cggcgaatta aatgctggta
     2701 agcaattatt tcttcacccc cgataatttt cgataaagtg gctttggtat cggttgtagt
     2761 agatttaaca atattcaact cagcctcaaa cgacggatag tccaaaatga tattaaacat
     2821 aaacctgtcg agctgtgctt caggtaaagg ataggttccc tcctgttcaa tcgggttttg
     2881 tgtagctaat acaaaaaacg gtaaatcgag tttatgtaaa tgcccaccaa tggtcacatt
     2941 tttttcctgc atggcttcta ataatgcagc ttgtgtttta ggtggtgtac ggttaatctc
     3001 atcggcaagg ataatattcg caaaaacagg tccttttaaa aatttaaagt tgcgattatc
     3061 atctaaaatt tcactaccgg taatatcact cggcattaaa tcgggtgtaa actggatacg
     3121 tttaaaggat aaatgtaatg cgtcagaaat ggttttaatt aataatgttt tagccaaacc
     3181 gggaacacca accaataaac tgtgaccgtc ggcaaaaatg ctcatcaaaa cattttgtac
     3241 aacttcgtcc tggccgataa ttacttttcc tatttccgcc ttcagccttt taaaatctgc
     3301 cgacagggca tcaatggctt ccacatcgtt tttaaattcc ttcataccgc tatattaata
     3361 caaatcaatt gaatatttgt ctgcaacaaa aataattaac gcattataac attacttact
     3421 aaccgtgttc aataatatgt cgaggttacc gcaattattg taagcagggt caactttaat
     3481 atattctttg gccgcttttt cttccaccca cgcacgcatt acagctactt gtttttgcga
     3541 cattgccgcg ttttgaattt ttgcgtaatc atcttttaaa ttcaatgagt gtggcgcaga
     3601 ctggtcgatt aatttaatga ggcgaattgc ttttttacca tcctgcgttg tgaaaggtaa
     3661 tacttgtgaa atttcgccgg gttttaaatt ttcaatggaa taataatcgt caggtgataa
     3721 ttcagcaatt tcgaaggtgc tttctcctgt ttgaggattc acaacagtac cacaagtttt
     3781 attgctgttt tcgtcgtcgc tgtatttttt tgtcgcctca caaaaagtta atttgcctgc
     3841 cacgatattt gctctgatac tatcgagttg tttattggct gcaacaaaat tgaggttggt
     3901 taccggtggc ataattaaaa tgtggcgcaa acaagcttta tcgccttggc ggctgtccat
     3961 tttaataatg tgatacccga attgtgtttc tactatttca gaaatttcgc cgggttgcaa
     4021 tttaaatgca gcagcagaaa attcggtaac atatttttcg cgtggctcac accctaaaaa
     4081 gccgcaatca tcttttgaac catcgccgga ataaatgctg gcaaggctac aaaaatcttc
     4141 tcctgcaata atacgttttc taatatcttc cgctttctgt tttgcaatgg ctttttgttc
     4201 ggcgttggct tgcggaataa tgagcaactg agcatattca atttccgcat taattaatgg
     4261 caacgaatct tttggtatgg cgttataaaa tgctttcacc tctgtaggcg atactgacat
     4321 ttcaccaaaa atattatcct gcatgcgctg aattaataat aaattgcgta catcatctct
     4381 gaattcctct ttaatttcct gaaccgattt gccataaaat tcttccattt tttcctgaga
     4441 gccgaaaagc gaaataaaat atcgaatacg gttttcaata ttactttcaa cctcatcatc
     4501 ggttacttca atactatctt tagcagcctg aataagcatg agtttttggg taaccaattc
     4561 ctgcattacc tggcagcgat aatcttccgg cacttctccc ttttgcaatt gctcaaaata
     4621 gaatgattct atttcgctca tcagaattat tttatcgcca acctgtccca caattttatc
     4681 tacaataggt ttatttgctg ttggttgagc aaatgctatt gcgctgataa aaacaagcaa
     4741 acaggtaatt attggttttt tcattgttct ttatttatgt attctgcgtt atttcttttt
     4801 aatccttcag tataaatgtc cttataggtt ttatccaata atgccatttt ccgtttatta
     4861 ataataattt ctttgatgcc ttttttttga tattcgaacg gacctaaatc tcctgccaaa
     4921 tgaaaatcct caactttaac aaaatacctg aaactagtat cccgataggt aacaattcct
     4981 tttgttctaa atttaccgcc ggcgtacata tcgtaaggca acaatttaaa caagtcgtcc
     5041 tcattcaacc aaatgtcgct gtttaacgaa aaactttcgc aattggctgc acagtaacgt
     5101 tcaattggca ggcgatattt ttcaataccc cgtgaaaacc aatattgtat ggaatcggcc
     5161 gacttaaccg cagcaggaac tactgcataa gctaactgat aaatatccgt ttttagggta
     5221 aaatcctttt ggttgttatc aaaataactg cgaatcgaat cgtcaactac aacagtatcc
     5281 agattttcag ccagccattg acgctcgtaa gcataaataa ccaacgattc tttataggct
     5341 tcagcttgtt tgttgatatt ggcgagagca gattgtaggt tgtcttctgc tacgcgtaaa
     5401 atgagatttt gtcttaccca gttttcaacg tattcggaaa ctatgcgggc gctgtcctca
     5461 ggagaagttt tatcgttaat gagaccctga atatcggtaa cgtataaata tttatcgtac
     5521 actctagcca gtggttcctg gtctttttca ggtttatgcc tgttacatcc aagaaccact
     5581 gtgcttaata agagtgctat gtaaacattt ttaaagctca atttatttta tcgaaccatt
     5641 gaattaaata ccttttcatt tacactaacc ggatattttt tatggagttc ttcaatccat
     5701 tgtttttcca gttgatcctg ataagcagaa attacataac ctcttgcttc atcaagtgtt
     5761 tttggtgcag gggcaacaat tttatttatt ttcacaaagg tgattgaacc atttgcattt
     5821 aaaatatcac taccaatgcc aacagtttta tttaataaat ccacattgct gtcctgacca
     5881 ggtaaaaata atgctgattt aatagaaact aattcggttg aatcaacatt aaatttatcc
     5941 ataatagctt tatcactttg atttttgcct gctaatttgc gcactgcttt agcagttttt
     6001 acatcagcac aagtataaat agtggcatca attctctctt cccacatata ttcatttttg
     6061 tgagcttcgt aataattttt caaaccaatt gtatcggttg aacctttgct ccagattttg
     6121 cgctcaagca tggcaaataa aggtaaacca tcgcggtatt cctgcaataa tcgtttaaaa
     6181 tcaatattgc gttcgctgag gtcgtactca attaatgttt gttctaaagc ctgggtatat
     6241 aatttattat atttctcttc aatattttta tcgcgggaaa cacgctgatt tatttccaga
     6301 taactagcca tatcgctttg tgtaaagttg cggttgccaa tagagaacat tgttttgttc
     6361 atattagcag cctggctcat taaccaagta tttttaacaa aactcgaatc gagttttgcc
     6421 aacatttctt ttttagcttc tgaattttca gtaaaaggat atttagtttt tactttttca
     6481 acaaaatcgc tgcgtaaagt tttatattca ggtgaacgtt caatttttcc ttttaattca
     6541 tcttttactt cagcgaaagt accattaggt tttttctcaa ttaatttgat gatatggtat
     6601 ccaaaagtag tttgtaccgg agcagcaata tcgccggggt tttttaatgc gaatgcagct
     6661 tcctgaaaag gcaacaccat tttacctgtt ccaaatggtt ctaattttcc gccattggca
     6721 gcacttactt tatcttcaga atatcgtcct gctaattctt caaatgttgc cggatttgat
     6781 tttactaaat tgtaaatact atcaatttta acttttgctt tggcaatatc ttcgggtttt
     6841 gcttcttttc cggttttaat taaaatatgt gcaacagtaa ctgttccttg agatgggcgt
     6901 tctttagcca ctttaataat atgataaccg aatttagatt taacaatatt gctggttgtt
     6961 ccaaccggtg tttcgaaagc cgcattttca aaattagcat ccggaatttg acctgcggta
     7021 atccatccaa ttaaacctgg gtcggtagca tttgttttat ccgtagaaac ttcagcagct
     7081 actttgccaa aatcttcacc tttttttaat ctttctgcag ctgcttttaa tttattaata
     7141 gcacctgtag tatcagctcc cggagcagcg gcatcgatag caatcataat atgatatact
     7201 aaacgttcgc tttgctgacg gttatacatt tttaaagccg cagcttccaa aacttcttta
     7261 tcgaagttgg atttaattaa ctggtcgcca tatttctgta attcttctct aactttttca
     7321 gtagtgtcga tgcgttcggc acgagcttca gcaactttaa gtttgaaatt gatgtacaaa
     7381 tcgaggtact catctaaact ttctttagag taatcgtttt gtttgtcggg attgtttttc
     7441 ttgtaaacat aaaggaattc ttccttcgta actggttgtc cggcaacggt aaataatacc
     7501 tcatttccac cggttgattg tgcgtttaca gaagtaataa gcagtaaacc tgcaagcagc
     7561 attaaagtct tgaaaatgtt cattttcata tgtggtaaat tatattttat aggtttgatt
     7621 gaattaaggg actgcaaagc taatgattat tagggtttaa gcaacataaa gggtaatgat
     7681 attgctcaaa acagcctgat cataggattg aaatccccgt ttcaaccgcc ctttacagca
     7741 aaaatcatgc gatattaacg gcattttatc cttacctttg acgccttaac aaattttaag
     7801 tcatgtcaat ttcaaaaaac aaagtggttt ccctgacgta tgaattgcgg gaagataatg
     7861 taagcggaga cctcatcgaa gtggttaaag ccgatcagcc attcgtgttt cttttcggcg
     7921 caggcggctt actgcctgag tttgagtcta atcttgaggg taaaatcatt ggaagcaatt
     7981 ttgaattcca aatcctgtct gcaaacgctt atggcgagtt cgatgaaact gccatggaat
     8041 atgtgcctaa ggatattttt atgatcgacg gccaattagc tgatgacctg cttgaggtag
     8101 ataaagtaat taacctacgc gatcagggtg gtcaccttgt tagagctcgt gttgctgaag
     8161 ttggcgaagc agaagtatta ctggatttca accatccact tgccggagtt aacctgcatt
     8221 ttaaaggtga aattctggac atccgcgaag cttctcagga agagattgaa cacgg
//
LOCUS       DAORKW010000102         8274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_239_length_8274_cov_4.453096, whole genome shotgun sequence.
ACCESSION   DAORKW010000102 DAORKW010000000
VERSION     DAORKW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_239_length_8274_cov_4.453096"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..471
                     /locus_tag="PLB46_05830"
     CDS             223..471
                     /locus_tag="PLB46_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRF76069.1"
                     /translation="MKKDIHPDNYREVIFKDFSADIAWLGKSTANTKETMTWEDGKDY
                     PLIKLEVSNVSHPFFTGKMKVLDTAGRIEKFNKRYSKK"
     gene            547..1755
                     /locus_tag="PLB46_05835"
     CDS             547..1755
                     /locus_tag="PLB46_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HRF76070.1"
                     /translation="MQIILFDDKRWQQLLPLTFTRPVADLRIGIDTIREKWESQLSGN
                     YSWLPEPYLNGKFPVGYDTDNYYIYGGVIPTPKLIAAVSNLQNDEVLVQNGEILASRS
                     QEHYFSGMSLCERAATLTQVEYPHELIIITRPWHLFQHNDSVLRQDFERMTKGRKSQD
                     ITANNTIIGNNIFVEPGAKVEAATINTNAGPVYISNDAEIMEGSLIRGPFALGKHSTV
                     KMGAKIYGATTVGPHCKVGGEVNNVIFQSYSNKGHDGFLGNSVIGEWCNLGADTNSSN
                     LKNNYAEVKIWDYQTQKFIPTGLQFCGLIMGDHSKCGINTMFNTGTVVGVNANIFGDG
                     FPRNFIPSFSWGGAAGFVTYKLSDAMIVAKDVMARRTVTLAEADKKILTTIYELSEKY
                     RKGQLIFLHK"
     gene            1800..2561
                     /gene="tpiA"
                     /locus_tag="PLB46_05840"
     CDS             1800..2561
                     /gene="tpiA"
                     /locus_tag="PLB46_05840"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRF76071.1"
                     /translation="MSRKKIIAGNWKMFKTLTEAELLVAEINNKLPKNLANAEVWIAP
                     PALYLPTLTKQFSESGIQFGAQDISKHQQGAYTGEISAMMLQSVSAKFTLVGHSERRQ
                     YHHEDDVLIGEKIHAALSQSITPVYCCGETLQERNSNNHFVIVEEQITTALANCTTEH
                     VQQLVIAYEPVWAIGTGVTASAGQAQEMHAFIRSILAQKYGASIANTIRILYGGSVKP
                     NNASELFSQPDIDGGLIGGASLVAADFIAIIESAI"
     gene            2563..3396
                     /gene="prmA"
                     /locus_tag="PLB46_05845"
     CDS             2563..3396
                     /gene="prmA"
                     /locus_tag="PLB46_05845"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRF76072.1"
                     /translation="MQYLEYKLITADDQTAEILMALLAEEGFDTFDQNYNTLSAYIPE
                     KAGNEITIANYLAELQQRFVFTWSQSQFEQRNWNEEWEKSFQPIVVSNTCLIRAPFHP
                     ARPEIAFDILIEPRMSFGTGHHESTYLMMEEMLGLNFNKKSVCDAGCGTGILSVLAVK
                     KGAQHVLAIDIEEWAYRNAMDNIALNGIYDQIDVELGDFSLMEGKQFDIILANINKHI
                     VLGNIQQFFRSIQPGGILIISGILQNDIQDVEKEASNYFFQLISTRTKNDWLCAVFQN
                     V"
     gene            3504..7979
                     /locus_tag="PLB46_05850"
     CDS             3504..7979
                     /locus_tag="PLB46_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76073.1"
                     /translation="MKKLFGIILFLFGMSTAFGQLKQFSYEQDQFLKELDDFMKTVNT
                     EITKDSYNRFAKEVEAGNITPDQLRLVTELCNKMLERKMKPTPQFVAVLDATVSFTKS
                     NQIMDRYEEWMLITNNILDNSKKGSYVTFNRFLDFSAAFFEKNALNDTPTRTWCYSSF
                     EYQFKLNGEIPEVHFDGTDIYGYTKGDTMYINGTKGVYYPLEKEWVGKSGSVTWTRAG
                     LDSTTTYGKLNNYKVNTESNEYVADTATLYFGGMFSKPLVGKLTDRLITNNEPIKSDY
                     PQFQTYDKTLVVKDILVNVDYKGGFLLKGGQIIGSGDDSIPAEMTFKNFEAKKAVIAK
                     SKTFLVEPAMSIYSKDAEVIIKVGKDDSIYHHDVSLRYDGVMGSMTITRQDEGFSSLM
                     FYSSYHKMDVKVDNLSWALSDTIIKFKNIEGAGQKMSWFESQDLFSAKLFERVRGVAT
                     YQPLVKIKRYCEMYKTREIPTLALAKELNPSLTIDGVQSLLLQLVEEGFIFYDKQKEM
                     VYVKDKTFNYVFASAGKKDYDIIAIPSVTKEPNALLNLKNYDLNVNGISQFYLSDSQF
                     VLIYPKDDKLTIKKNRDMLFDGTIVSGNIDFKGTNFYFSYDTFNIKMNAVDSMILYVE
                     STEIDDNGNPLYLPVNTNISVTSGRLQIDKTDNKSSKVHYKEYSIFTTYDYSIADYEK
                     PNIFDNVYDKEEFYFKLDPFTLESSDSLDYRKVKFDGTMISDGIFPDFREALKLQEDR
                     SLGFKTTTPAGGFPTYGKLGKYTGNLSLSNQGFRGSGTIDFMASKSQSSDIIFFPDST
                     LAKVEDFTVAKTESGVQFPAGSNTGVTTRWLPYEDKMIVQQGTKPFDLFENQATLKGT
                     AIVTSKGLEGDGTMFWGEVKLKSNQFKYKANNALSDTASVTIGQVTPDLVALKLDNVN
                     MDVDFKERIGHFKANYDSIMTELPYNEYKTDMNVFDWVMDERTVYFKSTKEYSNFYST
                     NGGQDSLVFQAKEGKFDLNTYLLNAEGVPLIKVADVHVIPDSGKVYIEKGAKMGTLYY
                     AKIKGDTLNFYPNLHNCVVNIMGRLAMSGTGEYEYITPQNVKQIIPIESITVQKQVDT
                     IMEVTTHHMFAKGYVQDSANFVLENKVRFKGPVELYSQDQFLKYRGYVKLDVLDTANI
                     RTSWFRINSYIDPKNPIFNLDDAVTERNDSVYAGIFRFNDTAALYTSIMSKKASIADA
                     QLFVARGSGYYNETDQTFYLGNLEKLDDPTAKGSILKYNDKTGALYGEGRLKMGLKTD
                     LCDTKAYGTIIKRPNNTNYRMDATVTFEIPLPGELMNKLGEMLLVSNSDAGAAPLDYN
                     VETQIVAAITELMDEKSYNKLMSDVSSKGYLDRPKDSPVSVFLSGIKMVWDPTSRSFH
                     TTGSDGKIVWFGDKAFNQHIKCYAEFGKRSGGDYFTIYLVTPSEDFVYISYKRNQLKL
                     FTSREDLNTEIFSYDPKKRVIETEKGNMVFSLEGKNAVNQFVQDMQYYEGLKENK"
BASE COUNT         2822 a         1377 c         1603 g         2472 t
ORIGIN      
        1 ggtcggagcc aatagaaacc ttcagtttat tgcccgcagc tgcctctttt tcaatggttt
       61 cacgcacctc atcttcaatc ggacgcgtta tttcaacacc gttaaatttg cgccatttca
      121 tagattgttc agtttactct acaatattaa tacgaaaata ttagaaaata ttaccaactt
      181 ctttaagaaa ttactaattt tgcaccttca attaacagaa caatgaaaaa agatattcat
      241 ccggataatt acagagaagt tatttttaaa gacttcagtg ctgatatagc ttggttaggc
      301 aaatcaactg ccaacactaa agaaaccatg acttgggaag atggtaaaga ttatcctctc
      361 atcaaattgg aggtttccaa tgtaagtcac ccattcttta caggtaaaat gaaagttttg
      421 gatacagcag gtcgtatcga aaaattcaac aaacgttatt caaaaaagta attttcataa
      481 taaattcata caaccccttt ggtgttaata tcatcaaagg ggtttttcat ttaaatttgt
      541 gcctgcatgc aaattatatt attcgacgac aaacgctggc aacaattatt gccacttact
      601 tttacccgac ctgttgctga tttacgcata ggtattgaca ccattcgcga aaaatgggaa
      661 tctcaattat ccggcaacta ttcctggtta cctgaacctt acctaaacgg taaattccct
      721 gttggttatg atacagataa ttattacatc tatggtggtg taattccaac accaaaattg
      781 attgctgcgg tttccaatct tcaaaatgat gaggttttgg ttcaaaatgg tgaaatacta
      841 gccagccgct cacaagaaca ttacttttcc ggaatgagtt tgtgtgaacg cgctgctact
      901 ttaacgcaag tggaatatcc gcatgaactc atcattatta caagaccctg gcatttattt
      961 caacataacg attccgtttt gcgtcaggat tttgaacgta tgaccaaagg aaggaagtcg
     1021 caggatatta cagcaaataa caccattatt ggcaataata tttttgttga gcctggtgct
     1081 aaagtggaag ctgctactat taatacaaat gcaggacctg tttatatcag caacgatgca
     1141 gaaattatgg aaggcagttt aattcgcgga ccttttgcat taggtaaaca cagcactgta
     1201 aaaatgggag caaaaatata cggcgctact actgttggtc cacactgcaa agttggtggc
     1261 gaggtaaata acgttatttt tcaatcgtat agtaataaag gtcacgacgg atttttaggt
     1321 aatagtgtaa ttggtgaatg gtgtaattta ggtgccgata ccaatagttc gaatttgaaa
     1381 aataattatg cggaagtaaa aatttgggat tatcaaacac aaaaatttat tccaacggga
     1441 ttacaattct gcggtttaat tatgggcgat catagtaaat gcggcattaa taccatgttt
     1501 aataccggta ccgtagttgg tgtaaacgcc aatatttttg gagatggatt tccaagaaat
     1561 tttattcctt catttagctg gggcggtgct gccggatttg tcacctataa attaagtgat
     1621 gccatgattg ttgcgaaaga tgtaatggca cgacggacag ttacattagc tgaagctgac
     1681 aaaaaaatac ttactaccat ttacgaattg agtgaaaaat acaggaaagg tcagttaata
     1741 tttttacata aataatagtt gataataatt tcaatcaact tacaaataaa attcaagata
     1801 tgtcgagaaa aaagataatt gcagggaatt ggaaaatgtt taaaacatta actgaagcag
     1861 aacttttagt tgcagaaata aacaataaat taccaaaaaa tttagcaaat gctgaagtct
     1921 ggatagcccc accggcgctg tatttaccga cattaaccaa acaattttct gaatccggaa
     1981 ttcaatttgg tgcgcaggat atcagcaaac atcaacaagg tgcttacacc ggtgaaattt
     2041 cagcgatgat gttacaaagt gtatcggcta aatttacatt ggttggacat agtgaacgca
     2101 gacaatatca tcatgaagat gacgtattaa tcggagaaaa aatccatgct gctttatcac
     2161 aatctatcac acctgtttat tgttgtggtg aaacattaca agagcgcaac agcaataatc
     2221 attttgtaat tgttgaagaa caaattacca ctgcattagc aaattgtacc acagaacatg
     2281 tgcaacaact tgtaattgct tacgaaccag tttgggcaat tggcacgggt gttacggcaa
     2341 gtgctggaca agcccaggaa atgcatgcgt ttattcgctc tatattggca caaaaatatg
     2401 gtgcatccat tgcaaatact attcgtattt tatacggagg aagtgtgaaa cctaataatg
     2461 catctgaatt attttcgcag cctgatattg atggcggact aattggtggt gccagtttag
     2521 ttgctgctga ttttattgcc ataattgaaa gcgcaatata acatgcaata tctcgaatat
     2581 aaattgatta ctgccgacga ccagactgct gaaattttaa tggcattatt ggctgaagaa
     2641 ggtttcgata catttgatca aaattataat accttaagtg catatattcc tgaaaaagcc
     2701 ggaaatgaaa taactatcgc aaattattta gctgaattac aacaacgatt tgtattcacc
     2761 tggtcgcaaa gccaatttga acaacgcaac tggaacgaag aatgggaaaa aagttttcaa
     2821 ccgatagtcg tttcaaatac ctgtttaatt cgcgccccat ttcaccctgc gcgtcctgaa
     2881 atcgcttttg atatcttaat cgaaccaagg atgagtttcg gtaccggaca tcatgaaagt
     2941 acgtatttga tgatggaaga aatgctgggg ttgaatttta ataaaaaatc ggtttgtgat
     3001 gccggatgtg gcaccggaat attatctgta ttggctgtta aaaaaggcgc tcaacatgtt
     3061 ttagccatag atatagaaga atgggcatat cgtaacgcaa tggataacat agcgttaaat
     3121 ggcatctacg accagattga tgttgaattg ggggatttca gcctgatgga aggcaaacag
     3181 tttgacatta tacttgccaa tatcaataaa catatcgttt taggtaatat tcagcagttt
     3241 ttccggtcca ttcaaccggg aggaatttta attatcagcg gtatcttaca aaacgacata
     3301 caagacgtgg aaaaagaagc gtccaattac ttcttccaac taatatccac acgcaccaaa
     3361 aacgactggt tatgtgcggt tttccaaaat gtgtaggcaa attaggcatt acgatgttta
     3421 ataagtgtta acagtataat ttctccacgt tatttccgtt ttaacataag gcctaattac
     3481 aatagatatt ttaacttaac ggcatgaaaa aacttttcgg tattatcctg tttctctttg
     3541 gaatgagcac tgcttttggt cagttaaagc agttttcata cgaacaagac caatttttga
     3601 aggaattgga cgattttatg aaaaccgtga acacagaaat caccaaagac agctataacc
     3661 gtttcgccaa agaagtggaa gccggaaata ttacgcccga ccagttgaga ttggttaccg
     3721 agctctgcaa taaaatgctc gagcgtaaaa tgaaacctac ccctcagttt gttgcggtac
     3781 tggatgccac agtttcattt acaaaaagta atcaaattat ggaccgctat gaagagtgga
     3841 tgttgattac caataacatt ttagataatt cgaaaaaagg tagttatgtc acctttaatc
     3901 gtttcctgga tttctctgca gcttttttcg aaaaaaatgc actaaatgat accccaacaa
     3961 gaacatggtg ttacagttct ttcgaatatc agttcaaatt aaacggagaa attcctgaag
     4021 tgcattttga tggcaccgat atttatggct acaccaaagg tgataccatg tatataaatg
     4081 gtactaaagg tgtgtattat ccgttggaaa aagaatgggt agggaaatca ggaagtgtta
     4141 cctggacacg tgcaggatta gatagtacaa caacatacgg taaactcaac aattacaaag
     4201 taaatactga atcaaatgaa tatgttgccg atactgcaac cttgtatttt ggtgggatgt
     4261 tcagtaaacc cttggtagga aaattaacag accgattaat tactaataat gaaccgatta
     4321 aaagtgatta tccacaattt cagacttatg ataaaacatt agtggtgaaa gatattttgg
     4381 tgaatgtaga ttacaagggc ggatttttat taaaaggagg gcaaattatt ggtagtggcg
     4441 atgattctat tcctgctgaa atgactttca aaaattttga agcgaagaaa gccgtcattg
     4501 ctaagtccaa aacattttta gttgaacctg ctatgtccat ttattccaaa gacgcagaag
     4561 taataattaa agttgggaaa gatgacagta tttatcacca tgacgtttcg ttgcgttatg
     4621 atggtgttat gggttcgatg accattactc gtcaggatga gggattttcc agtttaatgt
     4681 tttacagttc ctatcataaa atggatgtaa aagttgacaa cttgagttgg gctttgagtg
     4741 ataccattat taaattcaaa aacatagaag gtgccggtca aaaaatgagt tggttcgaaa
     4801 gtcaggattt atttagtgcc aaactttttg aacgtgtgcg tggtgttgca acctatcagc
     4861 cattggtgaa aataaaaagg tattgcgaaa tgtataaaac ccgcgaaata ccaacactgg
     4921 cgttagcaaa agaattaaat ccttccttaa ctattgatgg tgtgcaaagt ttattattac
     4981 aattggtaga ggaaggtttt attttttacg acaaacaaaa agaaatggta tatgtaaaag
     5041 ataaaacatt taattatgtg tttgcctctg ccgggaaaaa agattatgat atcattgcca
     5101 taccatctgt tacaaaagaa ccgaatgctt tactcaattt aaaaaattac gatttaaacg
     5161 taaatggtat cagtcagttt tatttgagtg attcacaatt cgtactcatc tatccaaaag
     5221 acgataaact taccattaaa aagaacaggg atatgttgtt tgacggaaca attgtctccg
     5281 gtaatatcga ctttaaagga actaattttt attttagtta cgacacattt aatataaaaa
     5341 tgaatgcagt tgactctatg atcttatatg tagaatcaac tgaaattgat gataatggca
     5401 atccattata tttaccggta aataccaata tcagtgtaac ttcagggcga ttacaaatag
     5461 ataaaacaga taataaatcg agcaaagtac attataaaga atacagcatt tttacaacct
     5521 acgactattc catcgccgat tatgaaaagc ccaatatttt tgacaatgtg tatgataaag
     5581 aagaatttta ttttaagctc gacccattca cattagaaag ttctgactcg cttgattatc
     5641 gaaaggtgaa gtttgatgga acgatgattt ctgatggcat atttccggat ttccgtgaag
     5701 cattaaaatt gcaagaggat cgttcactgg gtttcaaaac aactactcct gccggaggat
     5761 ttccaacata tggtaaatta ggcaaataca caggaaattt atcgctaagt aatcaaggtt
     5821 tcagaggctc gggaacaatt gatttcatgg cttctaaatc gcaatccagc gacattatat
     5881 ttttcccgga ttcaacatta gctaaagtag aagattttac tgtagcaaaa acagaaagtg
     5941 gtgtgcaatt tcctgcaggt tcaaataccg gtgttacgac acgctggttg ccttacgaag
     6001 acaaaatgat tgttcaacaa ggtacaaaac ctttcgattt atttgaaaat caggcaacat
     6061 taaaaggaac agcaattgta acttcaaaag gattagaagg cgatggcaca atgttttggg
     6121 gtgaagtaaa attaaaatcc aatcaattta aatacaaagc caataatgca ttatccgaca
     6181 ccgcaagcgt taccattggt caggtaacac cggacttagt tgctttaaaa ctggataatg
     6241 taaatatgga tgttgatttt aaagaacgta ttgggcattt taaagccaat tatgatagta
     6301 tcatgaccga attgccttac aacgaataca aaaccgatat gaacgtattc gattgggtga
     6361 tggatgaacg aacagtatat tttaaatcaa caaaagaata ttcgaatttt tattctacca
     6421 atggtggaca ggatagtctc gtatttcagg ctaaagaagg taaattcgat ttaaatacgt
     6481 atttattaaa tgcagaaggt gttccgttaa ttaaagttgc cgatgtgcat gttattcctg
     6541 attccggaaa agtgtatatc gaaaaaggag ccaaaatggg aaccttgtat tatgcgaaaa
     6601 ttaaaggtga tacactcaac ttctatccta acctacataa ttgtgtggtc aacattatgg
     6661 gacgattggc catgagtgga acaggtgaat atgaatatat tacgcctcaa aatgttaaac
     6721 aaataattcc aattgaaagt attactgttc aaaagcaagt tgatactatt atggaggtaa
     6781 caactcacca tatgtttgcg aaaggatatg ttcaggactc agccaatttt gttttagaaa
     6841 ataaagtgcg atttaaagga cctgtggaat tatacagtca ggaccagttt ttaaaatatc
     6901 gtgggtatgt aaaattagat gtattggata ccgcgaatat tcggactagt tggtttagaa
     6961 ttaattcata tatagacccg aaaaatccga tatttaattt agatgatgca gttactgaac
     7021 gcaacgactc tgtgtatgct ggtattttcc gttttaatga tacggcagca ttatacacaa
     7081 gtataatgag taaaaaagca tcgattgctg atgcgcaatt atttgttgcc agaggaagtg
     7141 gttattacaa tgaaaccgac caaacttttt acttaggtaa tttagaaaaa ttggatgacc
     7201 ctacggctaa aggttctatt ttaaaataca atgataaaac cggtgcttta tatggtgaag
     7261 gtcgattaaa aatgggatta aaaaccgatt tgtgtgatac caaagcatat ggcactatca
     7321 ttaaacgacc caacaacaca aattatcgaa tggatgctac agtcaccttc gaaattccat
     7381 tgccgggcga gttaatgaat aaactgggag aaatgttatt ggtatctaat tcagatgcag
     7441 gtgctgcccc actcgactat aatgtggaaa cacaaattgt tgctgctatc acagaattaa
     7501 tggatgaaaa atcatacaat aaattaatga gtgatgtaag ttctaaagga tatctcgaca
     7561 ggccaaaaga ttctccggtg tctgtatttt tatccggaat taaaatggtt tgggatccaa
     7621 catcacgttc attccatacc accggttctg atggcaaaat tgtttggttt ggtgataaag
     7681 catttaatca gcatattaaa tgttatgccg aattcggaaa acgaagcggt ggcgattatt
     7741 tcaccatata tctggtaaca ccgagtgaag attttgtata catcagctac aaacgcaatc
     7801 aattaaaatt atttacctca agggaagatt taaataccga aatatttagt tacgacccta
     7861 aaaaacgcgt aattgaaact gaaaaaggaa atatggtatt cagcttagaa ggtaaaaacg
     7921 ccgtaaatca atttgttcag gatatgcagt attatgaagg gttgaaggag aataagtaat
     7981 tagctaatta gcaaattagt taattagcta attggtttac gacttcggaa gtgaatgata
     8041 atctgctgtc gcagattatg gtgggtaatg gattgttggg attgtattcc aaggtttttc
     8101 aagattatat cccacctccg aacccggctg tttccattgg tacatccgca cattggtgca
     8161 tcggtacatt aatcagcaca ttagcacatc accacatcag cacattggta cattaaatag
     8221 tcaacacgtg ggttgacggc tacggtaata ccgaattaca ttccacctcc gaac
//
LOCUS       DAORKW010000103         8205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_243_length_8205_cov_5.009939, whole genome shotgun sequence.
ACCESSION   DAORKW010000103 DAORKW010000000
VERSION     DAORKW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_243_length_8205_cov_5.009939"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PLB46_05855"
     CDS             complement(<1..898)
                     /locus_tag="PLB46_05855"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRF76074.1"
                     /translation="MNKPLAAITGVYGYVPEYRLTNAELEKMVDTSDEWIMTRTGIKE
                     RRILKGEGLATSDMVVEAVKGLLEKTGNKATDIDMLLVATTTPDMPFPATANIACDKL
                     GCNHILSYDISAACSGFLYALTTGAKFIETGMYKKVVIVGADKMSSIINYTDRTTCVI
                     FGDGAGAVLLEPSTDRNGIIDSVLKSDGSGRHYLHQKAGGSLKPASVETVAANEHTVY
                     QEGKTVFKFAVKNMADVAEDIMKRNNLKSEDIAWLVPHQANLRIIDATAERMGLPKDK
                     VMINIEKFGNTTAGTLPLCLWEW"
     gene            complement(937..1116)
                     /gene="rpmF"
                     /locus_tag="PLB46_05860"
     CDS             complement(937..1116)
                     /gene="rpmF"
                     /locus_tag="PLB46_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRF76075.1"
                     /translation="MPNPKRRHSKQRTLTRRNHHGADMPNVANCPNCGAPVLRHRVCS
                     ECGNYRGKIAIEKKA"
     gene            complement(1128..1670)
                     /locus_tag="PLB46_05865"
     CDS             complement(1128..1670)
                     /locus_tag="PLB46_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRF76076.1"
                     /translation="MNELREYQIPFVGMKLGLHHFNFELEETFFSHFEGSEVNKGKVF
                     IDLEFDKKERLFVLKFDISGSITTECDRCGQEFDLPIHGNHTLYVKIGDKREEDVDNE
                     EVIWIPESESVLDLAEIIYEFVHLTLPIQRIHPDLRDGKPGCDPEILKLLATEDEGAG
                     ENDNSNKDPRWDILNNLNTN"
     gene            complement(1784..2848)
                     /gene="pdxA"
                     /locus_tag="PLB46_05870"
     CDS             complement(1784..2848)
                     /gene="pdxA"
                     /locus_tag="PLB46_05870"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRF76077.1"
                     /translation="MDNKIKIGFTLGDVNGIGPEVLIKALEDKRILGICTPVIYGSNR
                     IISFYKKMINAEGFSYTTCATADQANSKAVNIINCITEEIILTPGQETEVGAKAALVS
                     LEAASDDLINGKIDALVTAPINKNNLHTVGFKYPGHTEYFNARANNKESVMMLMNENL
                     RVGLVTNHLPLKDVAGAITKELILQKIQIIYQTLKQDFALFNPTIAVLGLNPHSGDNG
                     LLGQEEKNIIIPAIKEAQDKKILAVGPFSADGFFGSGNYQQFDAILAMYHDQGLVGFK
                     SLSFGSGINFTAGLPFVRTSPDHGTAYDLAGKNTASADSMLHAIYAAIDICRNRADYL
                     ESTKNPLKRTYVEAERGKVM"
     gene            3133..3312
                     /locus_tag="PLB46_05875"
     CDS             3133..3312
                     /locus_tag="PLB46_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76078.1"
                     /translation="MVVISFDIHLWRLPVVINVPDRNIILESIALPENQKVTSIHLLV
                     NYNYPDSKKYLRIAF"
     gene            complement(3599..6034)
                     /locus_tag="PLB46_05880"
     CDS             complement(3599..6034)
                     /locus_tag="PLB46_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HRF76079.1"
                     /translation="MKLFYHAILGLFIGVSAANAQVENIAPLAQKINALIQLNESPVY
                     FAPFEEPDFDVPKHESIVKNATYFEVNESILNSILNNHFGFIEIAIPFKDGMLQVQLI
                     PSQLFTPDFKVTNSNNEFFNYEGGAYYKGIVKGDNESIVAMSFYNNRIKGIISTKKDG
                     NIVVANLRSSPIHTIYQDRDLIITNPFVCTSDDKDILIDLNDVADGVEAIDPCLRVYI
                     ETDYQLYQEEGGIIQAADQIVDLFNVVSTLYYNEDITIIISEIYVWVTEDPYPHGSSD
                     DALAYFRDYRTTFDGNLAHLVTYDVENLGGIAYLPGVCTSYKYAYSNIDNYFADFPTF
                     SWTTYVFTHETGHNLGSPHTQNCSWPGGAIDNCYETEGACSPGPAPVDGGTIMSYCHL
                     TPTGINFANGFGPLPGDLIRDTYGDVGCLDACDLPPANDWPCDATILPVNGECIFIDG
                     SNIGAINSVVANVACDGISEGDVWFKVTMPPVGYVIIDTDNGAIVHNMGMNVYSGSCT
                     SLTSYPDGCVSNGSTYSPLMPGITLTGTPGVNYYLRLWEVGNDAFGSFSICAYTECAS
                     SYPADEINTTDPLICIGESTTLTVEGGALGDGGTWNWYSGSCAGTPVGTGESITVSPT
                     VPTTYYVKVIGDCNTTTCVSKTININPQPAVPVIIKTDCMLSVELVDGLSYQWFLDGV
                     LIAGETENTTTVLANGTYTVSVTNATGCVATSEALEVTCQGLNLQSINDASIQIFPNP
                     GNGNFTIAVEGMYGDITVSITDIAGQIISSQSHFISTTNNKLPIALNVASGIYFIKAG
                     KSEAFATAILIIE"
     gene            complement(6256..7146)
                     /locus_tag="PLB46_05885"
     CDS             complement(6256..7146)
                     /locus_tag="PLB46_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76080.1"
                     /translation="MKPHPDINSTFNSIKNLPVEIDYSGIEHFVMSQPATVFTLKTIF
                     SSTKIIFMTTGIATIITTAAIFLSINNKSETPVAATTLAIKKEISPEQPMVNIPQHTQ
                     QQSLENISPVPQEKKQQNTTAQIVLANPLNILFPDIAGNLQLDDTTKRKTSIAVSSGS
                     GYSYTNVSVGGSSNSSCTTSVISGSNATIMSYSGDNNEDETAVYNFTTYPCEKGNAYE
                     AIIEQALLADGYIKSDKKYKYEITSKGFSVDGDQKGVAARDKYYQLLIDNSCMILNGN
                     YAFEFAKKGDSISISTSSCD"
     gene            complement(7150..7698)
                     /locus_tag="PLB46_05890"
     CDS             complement(7150..7698)
                     /locus_tag="PLB46_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF76081.1"
                     /translation="MRSEAHDEFLALYQPVHERFVRYCSSHAYGIMETEDLVQETLLK
                     TMQHFNSLKDREKLLPFMISIANNVMHNVLRRRKFRGVYDEKAFSKLTAMSGNADVAM
                     DIHYLYKALNMLAEKDKEAIILFEITGYSIKEIAAIQSSNENATKTRLSRARQKLKSL
                     LEEEPVHQIKFAKAGQLFTLLM"
     gene            7712..>8205
                     /locus_tag="PLB46_05895"
     CDS             7712..>8205
                     /locus_tag="PLB46_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HRF76082.1"
                     /translation="MIAAPIETLICKRLHDGKFIILFFAKVMGNHFRHIKAFGQHFLR
                     NEETALQIVQALQPLTLAENILEIGPGLGVLTNHLKKITDKQLYVSELDKRIIAILKS
                     EQQFDDEHILEGDFLQSSPEKHINGPFMLIGNFPYNISSQILFKMLDHLELVPLMVGM
                     FQKE"
BASE COUNT         2456 a         1585 c         1442 g         2722 t
ORIGIN      
        1 cccattccca taaacacaat ggtagagttc cagcagtggt attaccaaat ttttcaatat
       61 tgatcattac cttatctttt ggcagtccca ttctttcagc agttgcgtca attatacgca
      121 aattggcctg atgtggaacc aaccaggcaa tgtcttcact tttcagatta ttgcgtttca
      181 tgatatcttc cgcaacatct gccatgtttt ttacagcaaa tttgaacact gttttgcctt
      241 cctggtaaac ggtatgctca tttgctgcaa ctgtttctac gctggctggt ttaagtgaac
      301 cacctgcttt ttgatgcaaa tagtggcgac cggaaccgtc agattttaac acggaatcaa
      361 taataccatt tctatcagtg cttggctcca gcaaaactgc acctgcacca tcaccaaaaa
      421 tcacacatgt agtgcgatcg gtataattaa taatggaaga cattttgtct gcacctacaa
      481 ttaccacttt tttatacatg ccggtttcaa taaacttggc accggtggta agggcataca
      541 aaaagccgct gcaagcggca ctaatgtcgt aacttaaaat atgattacag cctaatttat
      601 cgcaggcaat atttgctgtt gccggaaaag gcatatcagg tgtagtggtt gctaccaata
      661 acatatcgat gtcggtagcc ttattacctg ttttttcgag taaaccttta acggcttcca
      721 caaccatgtc ggatgtggct aaaccttctc ccttcagtat ccggcgttct ttgatgccgg
      781 ttcgtgtcat aatccactcg tcggaagtgt ctaccatttt ctcgagttcg gcattggtaa
      841 gcctgtattc gggaacataa ccgtaaacac cggtaattgc tgcgagcggt ttattcatac
      901 tgtgggtatt gattaactga aaatcccttt agtggattaa gcttttttct cgatagcaat
      961 tttacctctg taattaccac attcagagca aacgcgatga cgtaacaccg gtgcaccaca
     1021 atttggacaa tttgcaacat ttggcatgtc tgctccgtga tggttgcggc gcgtcaaagt
     1081 tctttgcttg gagtggcgtc gttttggatt tggcattgta ttaaatttta atttgtattt
     1141 aaattattca aaatatccca acggggatct ttattactat tatcattttc accggcacct
     1201 tcatcttcag tagccagtaa ttttaaaatt tcagggtcgc atcccggttt cccatctcgt
     1261 aaatccgggt gaatgcgttg aattggcaag gttaaatgca caaactcata aatgatttct
     1321 gcaaggtcca aaaccgattc actctccgga atccatataa cttcttcatt atcaacatct
     1381 tcctctcttt tatcgccgat tttaacatat aaagtatgat tgccatgaat tggtaaatca
     1441 aactcttgtc cacaacgatc acattccgta gttatacttc ctgaaatatc aaatttaaga
     1501 acaaataaac gctccttttt atcaaactca aggtcaatga aaaccttgcc cttgttcact
     1561 tcgctgcctt caaaatggct gaagaatgtc tcctccagtt cgaagttgaa gtggtgcaat
     1621 cccaacttca tgcccacaaa ggggatttga tattccctca actcgttcac ttcccgtgtt
     1681 ttttaaaaag gctgacaaag gtaaggaatg ataactaatt atggaagttt acagtaagaa
     1741 tagatataga aataggatga tttaccctcc tttagccact atatcacata actttccctc
     1801 tttccgcttc tacataagtc cgttttaatg gattttttgt gctttccaga taatcggccc
     1861 tgttgcgaca aatatcgatt gcagcataaa tagcatgtaa catgctatcg gcagaagcgg
     1921 tattttttcc tgcaaggtcg taagcagtcc catgatcggg tgaagtgcgc acaaatggca
     1981 aacctgcagt gaaattgatg ccgctgccaa aactcaaact tttgaagcca accagtccct
     2041 gatcgtgata catcgccaga atggcatcaa actgctgata gttaccgctg ccaaaaaagc
     2101 catcagcgct gaatgggcca actgctaata tctttttgtc ctgagcctct ttgatagccg
     2161 gtataattat atttttttct tcctgtccaa gaagtccgtt atcgccggaa tgcggattta
     2221 accccaaaac agcaatggta ggattaaata aagcgaaatc ctgttttaag gtctggtata
     2281 tgatttggat tttttgtaaa atcaactcct tggtaatagc accggcaaca tcctttagcg
     2341 gaaggtgatt ggtaactaac ccaaccctaa ggttttcgtt catgagcatc atcacgcttt
     2401 ccttgttatt agcccttgcg ttgaagtatt ctgtatgtcc cggatatttg aaaccaacgg
     2461 tgtgtaaatt atttttattg attggggcgg tcactaacgc atcaattttc ccgttgatta
     2521 aatcatcgga agcagcttcc agacttacta aagctgcttt ggcacctact tcggtttctt
     2581 gtccgggggt tagaattatt tcttcagtaa tgcaattgat aatgtttaca gccttcgaat
     2641 tggcttgatc tgcagtagcg catgttgtat aactaaagcc ttctgcgttg atcatttttt
     2701 tataaaatga aataattcgg ttggaaccat aaataacggg ggtgcaaatg cccagtatac
     2761 gcttgtcttc cagtgcttta atcaatacct caggccctat gccattaaca tcgccaagag
     2821 taaacccaat ttttatttta ttgtccatca acccgagttt attatagtgc gaaattaaac
     2881 atttgaagtg aagttgtaat atactaaatt tatcattgag actaattttt gtgacaaatg
     2941 caattgcttg ttaaactcgg aataggtttg gtggtatcgt gttttagcag ctgaggtatt
     3001 acaccacttc cttgtccggc tcccgataat taatttcatc agaataattt tggcactaaa
     3061 aatttggtat taaatgacca tctaaatgag cagcaataat cctaaatatt tccgtttaat
     3121 caccctgcta aaatggtggt catttcgttc gatatacatt tatggagatt gcctgttgta
     3181 attaatgtac cggacagaaa cataattctg gaaagtatag cattgcctga aaaccaaaaa
     3241 gttacatcaa tacatttatt ggtaaattat aattatcctg attcaaaaaa atatttacgt
     3301 atcgcctttt agctatttat tatacccaac atgccatttt attttagcat aaaataaata
     3361 taggccttgg tggtgtaaaa ttgtgttttg cctgaagtgg atgggaaatt aatttagtaa
     3421 cgcgatgggc aaatcaattt tgttcggaga aatatgattt atagcaacat tttttttctg
     3481 atattggcat tacttatttt ggagataaaa gtgaattgga aaatggaata attgatagcc
     3541 ggccaaaaca aaaaaagcac ttaaattcat aagtgctttt ttatagaaaa ttattttttt
     3601 actcaattat taatattgct gtagcaaagg cttcagattt tcctgctttt ataaaataaa
     3661 tgcccgatgc tacatttaat gcaattggca acttattatt tgtagttgaa atgaaatgtg
     3721 attgagagga aataatttga ccggctatat cggttattga tactgttata tcaccataca
     3781 ttccttcaac agctattgta aaatttccat tacccggatt tggaaaaatt tgtatactcg
     3841 catcatttat tgattgtaaa ttcagtccct gacaagtaac ttcaagggcc tccgatgttg
     3901 caacacagcc tgttgcatta gttacggaaa cagtataagt gccattggct aaaacggtgg
     3961 tggtgttttc ggtttcgcct gcaattaata ccccatccaa aaaccattgg taggataatc
     4021 catcaactaa ttcaacactg agcatacagt cagtttttat aattaccggg actgcaggtt
     4081 gtggatttat atttattgtt ttactcacac aggtcgttgt attacaatct ccaattactt
     4141 ttacataata agttgtcggt acagttggac ttacagtaat tgattctccg gttccaaccg
     4201 gagtgcctgc acaacttcca ctataccagt tccaagttcc accatcacca agtgcacctc
     4261 cttcaactgt taatgttgtg ctttcaccaa tgcatattaa agggtctgtg gtattaattt
     4321 catctgcagg atatgaactt gcacattcag tatacgcaca aattgaaaat gaaccaaatg
     4381 catcgttacc aacttcccac aagcgtaaat aataattcac tcctggtgta cctgttaaag
     4441 ttattcctgg cattaatgga gaataggtgc ttccattact aacacaaccg tcgggatatg
     4501 atgttaatga agtgcaagaa cccgaataaa cattcattcc catattatgc acaatagcac
     4561 cattgtctgt atcaataatg acataaccta caggtggcat tgttacctta aaccatacat
     4621 ctccttcact tattccgtcg catgcaacat tagcaacaac agaatttata gcaccaatat
     4681 tactaccatc aataaaaata cattccccat taacaggcaa aatagtggca tcgcatggcc
     4741 aatcatttgc aggtggtaaa tcacatgcat ccaaacaacc tacatcacca tatgtgtctc
     4801 gaattaaatc gcccgggagt ggaccaaaac catttgcaaa attgatacct gttggggtta
     4861 aatggcagta gctcattatt gtacctccat caacaggtgc cggtccgggt gaacaagctc
     4921 cttctgtttc ataacagttg tcaattgcac cacctggcca actgcagttt tgggtatgtg
     4981 gcgaacccaa attgtgaccg gtttcatgcg taaatacata tgttgtccag gaaaaggttg
     5041 gaaaatctgc aaagtaatta tcaatattac tgtaagcata tttgtaggaa gtgcatactc
     5101 ctggtaagta tgctatacca ccaagatttt caacatcata tgtaaccaaa tgagcgagat
     5161 ttccatcaaa cgttgtgcgg taatctctaa agtatgctaa cgcatcgtcc gatgatccat
     5221 ggggataagg gtcttcagtt acccaaacat atatttctga tataatgatg gtgatgtcct
     5281 cattataata taaggtagaa acaacattaa ataaatcaac aatttggtcg gcagcttgaa
     5341 ttattccacc ttcttcctgg tagagttgat agtcagtttc aatataaacg cgtaaacagg
     5401 ggtcaattgc ctcaacgcca tctgcaacat cgttcaaatc aatcaaaata tccttgtcat
     5461 cagaagtaca aacaaacgga tttgttatta ttaaatctcg atcctgataa atagtgtgga
     5521 ttggtgatga acgtaaatta gcaacaacaa tattcccatc ctttttggtt gaaataatac
     5581 ctttaattcg attgttataa aaactcattg ctactatcga ttcattgtcg ccttttacta
     5641 ttcctttata atatgcacca ccttcataat tgaagaattc gttatttgaa tttgttactt
     5701 taaaatcagg tgtaaacaat tgcgagggaa ttaattgtac ttgtagcatt ccatctttaa
     5761 atggaatagc aatttcaata aatccaaaat ggttgttaag tatggaattc agtatgcttt
     5821 catttacctc aaaataagta gcatttttta caatagattc atgtttgggt acatcaaaat
     5881 ccggctcctc aaatggtgca aaataaacag gtgattcgtt taattggatc agtgcattaa
     5941 ttttttgcgc tagtggggct atgttttcta cttgggcatt cgcagccgaa acgcctataa
     6001 aaaggcctaa aatggcatgg taaaacagtt tcattttttt gtattatgcc acaaatatag
     6061 ctgaactcat tggcacttgc attttttaac atttgaggtg agatataagt tgactaaagt
     6121 caatcgcctg ccatggaaaa cccgttttta ccctgtcact tttgatgtat aatgttaaat
     6181 ataaaattta acttaagcaa taaaataaca aggcgaacct tgaaagattc gccttgttat
     6241 ttgcaaagta aaaacttaat cgcaagaact tgtgctaata gaaatactat cccctttttt
     6301 agcaaattca aaagcgtaat ttccattcaa aatcatacag gaattatcaa tgagcaattg
     6361 ataatatttg tctcttgctg ctacaccttt ttgatcccca tcaacggaaa atcctttaga
     6421 tgtaatttca tatttatatt ttttatccga ttttatgtaa ccatcagcta acaatgcttg
     6481 ttcaataata gcttcatatg cattcccttt ttcacaaggg taagtagtga aattatacac
     6541 cgccgtttca tcttcattat tgtccccgct ataactcatt atggttgcat tactgccact
     6601 aattaccgat gtggtgcatg atgaatttga tgacccacct actgaaacat tagtatagga
     6661 ataacctgaa ccagaactta ctgctatgct tgtttttcgt ttggtagtat cgtcaagttg
     6721 caaattacct gcaatatcag gaaaaagtat atttaaagga tttgccaaaa caatttgtgc
     6781 tgtcgtattt tgttgctttt tttcttgtgg aaccggcgag atattttcca aagattgttg
     6841 ttgtgtatgc tgtggaatat taaccattgg ttgctcagga gaaatttctt tttttatcgc
     6901 taaagtggtt gccgctactg gtgtttcaga tttattgttg atcgataaaa atattgcggc
     6961 tgttgttata attgttgcta taccggttgt cataaaaata atttttgtag atgaaaaaat
     7021 agtttttaat gtaaacacag ttgcaggttg cgacataaca aaatgttcaa tacccgaata
     7081 gtcgatttca acaggcaaat ttttaatgct gttaaatgtt gaattgatgt ccggatgtgg
     7141 tttcatttgt tacattaata aagtgaataa ttgtcctgct ttggcaaatt taatttgatg
     7201 cacaggttct tcttccaaca atgattttag tttttgtctc gctctgctta accgtgtttt
     7261 ggtagcattc tcatttgacg actgaatagc tgctatttct tttatggaat aaccggtaat
     7321 ttcgaaaagg atgatcgctt ctttatcttt ttctgccagc atatttaatg ctttatacaa
     7381 ataatggata tccattgcaa cgtctgcatt gcccgacatt gctgtaagtt tgctaaatgc
     7441 cttttcgtca taaacgcctc tgaattttct tcttcttaat acattatgca taacattatt
     7501 cgcaatactt atcataaaag gcaataactt ttctctatcc tttaatgagt taaaatgttg
     7561 cattgttttt aataatgttt cctgaacaag atcttcggtt tccataatac cataggcatg
     7621 actgctgcaa tatcttacaa accgttcatg aaccggttgg tatagtgcta aaaattcgtc
     7681 atgtgcctct gatcgcatgt attcagttta tgtgattgct gctcctatag aaacattaat
     7741 atgtaaaagg ttacacgatg gtaaatttat tatccttttc tttgcaaaag taatgggtaa
     7801 tcattttcgt catatcaagg cttttggaca acatttttta cgcaacgaag aaactgcttt
     7861 gcaaattgtg caggctttgc aaccattgac cttagccgaa aatattttgg aaataggtcc
     7921 tggtttgggt gttctgacaa atcatttaaa aaaaattact gataaacaat tgtatgtatc
     7981 ggagttagac aaacgcatta tagccatttt gaaatcagag cagcaatttg atgatgaaca
     8041 tatacttgaa ggcgattttc tgcaatcttc acctgaaaaa catatcaatg ggccattcat
     8101 gctcatcggc aattttcctt ataatatcag ctcacaaatt ttgtttaaaa tgcttgatca
     8161 cttggaactg gtgccactta tggtagggat gtttcaaaaa gaaat
//
LOCUS       DAORKW010000104         8152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_245_length_8152_cov_4.318760, whole genome shotgun sequence.
ACCESSION   DAORKW010000104 DAORKW010000000
VERSION     DAORKW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_245_length_8152_cov_4.318760"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PLB46_05900"
     CDS             <1..519
                     /locus_tag="PLB46_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76083.1"
                     /translation="STFGIVAMDVNNDGNLDIVSHGNFYETEIETNTQDAGIGNVLLG
                     TGDGHFTPLHARYSGFYSCLNAKALAVITIGTNKTPVLLSTNNNNKMEAFKLVSNQKS
                     VALNNNDAYAIITFTDGKKRRQELYTGSGYLSQNSKSVIVNDQVAEITVYDNKGNARS
                     VYGSMLTLGSKK"
     gene            547..4164
                     /locus_tag="PLB46_05905"
     CDS             547..4164
                     /locus_tag="PLB46_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF76084.1"
                     /translation="MKLKNILLFAGAATLMFAASCKLFSKGDGDPRGTAIIDPKREVT
                     LDGNSGKLFDILTGKSTGITFVNDLRETYEVNWWRYSYIYNGGAVSIGDVNNDNLPDL
                     FFTGNLVESKLFLNKGNLKFEDITAKSGITTSKDKWPYGSSMVDVDGDNDLDIYVAMS
                     RWDKPERRTDEFWVNNGDGTFTEKAKEFGIADQSYSTHANFFDYDNDGDLDMYLATHP
                     VDFIDKNKTKYFQKIEQGKNLSNMFYINNGDNTFTESHMKVGINNHGYGLSATVGDIN
                     EDGFLDVFVSNDYAMYDYVYVNNGDGTFRDESLKAIKKTSINSMGTDISDFNNDGFLD
                     MFVVDMDMEDNYTYKTFMLSSQVEVMRILINAGYGYQNRSNSLQLNNGDGTFAEISRT
                     ANVSTTDWSWTTFFTDFDNDGWKDLYIANGFLRDFHVDESETYHKLRRAVRIEDSTVY
                     YEVKKELPTYVLDHPNYIFKNNGDLTFKDMRDEWGIYYPSITYGGGYADLDNDGDMDI
                     VTSNVNETPWVYRNNSEKMNTGNNWIQFKFKGYAKNTGGLGTKVKIYTGEQMQYIQHT
                     SSRGYITTVQDDCHFGVGKAQKIDMVEIEWLDGSKQILTDVPVNQVVMLDHANASKSV
                     KFIDRPKGDKVFVKKTEELKTDFAHQEAEYDDYLREFMLPHKMSTLGSGIAVGDVNGD
                     GADDYYIGGGAGQSGALYIQNANGSFEKSAWQQTAADGAASEDAGALFFDADGDGDND
                     LYVASGSNEFAANDAKYADRLYMNDGKGNFSKSGDALPGFKSSSSSVNGADFDKDGDI
                     DLFIGGRQVPGRYLAPASSYILQNNGGKFTDVTAQVAPMLKDCGMITSGLWTDFDNDN
                     NIDLILTGDWMPVTFLKNKGGKFTDVTKNTGLTNATGWWQSITGADMDNDGDMDYVVG
                     NFGTNRRYKNTTAVSDGHALPLEAFLYDFDKNGQEDYIMCYYQHDKLFPVKTRERMLE
                     QIPTMAEQYPTWGAYGDATVQDMFGDDLEKSIHRTAYIFHTSVLMNEGNGKFTMKFLP
                     NEAQISSTFGMVTDDFNEDGIQDILLHGNFYNTEIEITRHDAGTGLLLYGKGDGTFAP
                     QRGYITGFRSDGDSKALATIIDSKSKQPVYLLGNSNGKMESFGLIKTTQIITLAANDA
                     YAIVSYKDGKKSKVELYSGSGYLSQSAKMVRVTPNVAQIDVYDYKGQMRTVFPAALSA
                     K"
     gene            complement(4217..4858)
                     /locus_tag="PLB46_05910"
     CDS             complement(4217..4858)
                     /locus_tag="PLB46_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRF76085.1"
                     /translation="MQKFIPIFPLNLVAFPGEVINLHIFEPRYRQLIRECLEEEKMFG
                     IPVVENNRMLDYGTEMELVKIQKEYPNGEMDVKVKGVHVIQILETVKEVPGKLYDGAI
                     VSVIQNIEDRHSRLLAELEELTDTLFSLLDIKQDIYKPDFVLNSFKLGHYVGFEFMEE
                     YELLRHPRETSRQKLIVEHIKKILPSVKQIAEIKEKAKLNGHFRMINPPDTLK"
     gene            complement(4908..5525)
                     /locus_tag="PLB46_05915"
     CDS             complement(4908..5525)
                     /locus_tag="PLB46_05915"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HRF76086.1"
                     /translation="MKKNRLLCLLVIAVVLSACKQQSNEVVIQNDSAYFDAIRKSRAA
                     TNQYFMSASGPVSDSLKPNFEGVNYFPVDTNFKVIADFEKVNSNEVFEFSTTGKIADK
                     YKHIGDLIFTLQGVACKLAVYENQSLKAQGEVIYFVPFFDLTNSNETYGGGRYLDFKA
                     INTQKIIIDFNEAYQPYCYYNHSYSCPIPPLSNKLQVAVRAGERM"
     gene            5647..6006
                     /locus_tag="PLB46_05920"
     CDS             5647..6006
                     /locus_tag="PLB46_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRF76087.1"
                     /translation="MLDIEKFRSYCLAKKGVTEEFPFGEDTLVFKVMGKMFALTGLDA
                     ESFRINLKCDSEQITYLRAKYPAVQPGYHMNKQHWNTVMVDGSIPEKEVKAMIDHSYD
                     EVVKGMTKKQRQELENL"
     gene            6029..6766
                     /locus_tag="PLB46_05925"
     CDS             6029..6766
                     /locus_tag="PLB46_05925"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRF76088.1"
                     /translation="MLPPEILFEDNHLIAINKRPGEISQGDKTNDVPLVEIVKQYLKE
                     KYQKPGNVYAGLIHRIDRPVSGVLIFAKTGKAGERMSKIVRDRDFKKTYWAIVKNKPE
                     QESATLVHHLLKNERMNKSFAYTEKKGDSKEAILHYQLIASSDRYFLLEVDPQTGRHH
                     QIRAQLATIGSPIKGDLKYGFDRSNKDGSVSLHARQLEFIHPIKNEPVIITANPPVDP
                     AWNASMEILGHKHNPQSIPGYHKIFFL"
     gene            complement(6768..7595)
                     /locus_tag="PLB46_05930"
     CDS             complement(6768..7595)
                     /locus_tag="PLB46_05930"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRF76089.1"
                     /translation="MKKTLLTLSIILFVGAAAQAQQGIHFGFHGQMNSIWILNQNNYE
                     YSEMDYEYKFGPTGGLTLGYNWEDNFGIQVEFNYAQMGQDYSDIMKDFGPTEDTSKPD
                     LQTKVLTYRYVDLNYLQVPVLFKYMEGDTKDAIKYQLLGGLQFGYLLSAEQTYTADIM
                     NIDPDDQKPVNTAPQSGVPDFRGNASVNGTDYFQKLDVGLLLDVGADIYVNDKLYFSP
                     SFRGYYGFFDINAKETRNLQPAQGENIYLASHNAYIGFSLGINFMFPDAKLGGSDDE"
BASE COUNT         2735 a         1429 c         1492 g         2496 t
ORIGIN      
        1 agcaccttcg gtattgtagc aatggatgta aacaacgatg gtaatttaga tattgtttct
       61 catggtaact tctatgaaac agaaattgaa accaatacgc aggatgccgg tatcggtaac
      121 gtattgctag gaaccggtga tggccacttt acaccattac atgcgcgtta ttcaggattt
      181 tattcttgcc tgaacgcaaa agcattagcg gtaattacaa taggaacaaa caaaactccg
      241 gtattattat ctactaataa taataataaa atggaagcat ttaaattagt gagcaatcaa
      301 aaatctgttg cgctcaataa caacgatgct tatgcaatta tcacatttac tgatggcaaa
      361 aaacgtcgtc aggaattata taccggatca ggatatttaa gtcaaaattc aaaatcagta
      421 attgtaaatg atcaggttgc tgaaataact gtttacgaca acaaaggcaa tgcacgttct
      481 gtgtatggca gtatgttaac gctgggcagt aaaaaataaa tcaataacct gtattaatta
      541 ggcactatga aattaaaaaa catattatta ttcgcaggag cagcaacctt aatgtttgct
      601 gcctcttgca aacttttttc gaaaggcgat ggcgacccgc gtggtactgc tatcatcgac
      661 ccgaaaagag aagtaacttt agatggcaac tccggaaaat tatttgacat acttaccggc
      721 aaatcaacag gcattacttt cgtaaacgac ttacgtgaaa catacgaagt aaactggtgg
      781 cgatattctt acatttataa tggtggagca gtatctatcg gagatgtaaa taacgataac
      841 cttcctgatt tatttttcac aggaaatctt gttgaaagca aattgttcct caacaaaggc
      901 aatttaaaat ttgaagacat aactgctaaa tccggtatta ccacttctaa agacaaatgg
      961 ccgtatggtt catcaatggt agatgttgat ggtgataacg atttagatat ttatgttgcc
     1021 atgagccgtt gggataaacc tgaacgcaga accgatgagt tttgggtaaa taatggcgat
     1081 ggcacattta ccgaaaaggc aaaagaattt ggtattgccg atcagtcgta tagtacacat
     1141 gctaactttt tcgattacga taatgacggc gacttggata tgtatttagc tacgcatccg
     1201 gttgacttca tcgacaaaaa caaaacaaaa tattttcaaa aaattgagca gggtaaaaac
     1261 ctgagcaata tgttttatat caacaatggc gacaacacgt ttaccgaatc acacatgaaa
     1321 gttggcatta acaatcatgg ttatggtttg agtgctactg ttggtgatat taatgaagat
     1381 gggtttttag atgtatttgt ttcgaacgac tatgccatgt atgactatgt gtatgtaaac
     1441 aatggcgatg gcactttccg cgatgaatca ttaaaagcaa ttaaaaaaac atccatcaac
     1501 tcgatgggaa ccgatatttc tgattttaat aatgatggtt tcctcgacat gtttgtagta
     1561 gatatggata tggaagacaa ctacacttac aaaacattta tgttgtcgag ccaggttgaa
     1621 gttatgcgca ttttaattaa tgcagggtat ggttatcaga atcgctccaa ttcacttcag
     1681 ttaaataatg gtgacggtac ctttgcagaa atttcaagaa cggcaaatgt atcaacaacc
     1741 gactggagct ggacaacctt ctttaccgat tttgataatg atggttggaa agatttatat
     1801 atcgcaaatg gtttcttacg cgatttccat gttgatgaat ctgaaactta tcacaaactg
     1861 agaagagcag ttcgtattga agactcaaca gtttattatg aagtgaaaaa agaattacca
     1921 acttatgtgt tagatcatcc taactatatt ttcaaaaata atggcgattt aacatttaaa
     1981 gatatgcgtg atgaatgggg tatttattat ccttctatta cttatggcgg tggttatgcc
     2041 gacttagata atgatggtga tatggatatt gttacttcaa acgtaaacga aacaccttgg
     2101 gtatatcgca ataacagcga aaaaatgaat accggtaata actggattca atttaaattt
     2161 aaaggatatg ctaaaaacac cggtggctta ggtactaaag tgaaaattta taccggtgaa
     2221 caaatgcaat atattcagca cacttcatca cgtggttata tcacaaccgt gcaggacgac
     2281 tgtcattttg gtgttggtaa agcacaaaaa atcgacatgg ttgaaattga atggttagat
     2341 ggttctaaac aaattttaac tgacgttcct gtaaatcagg ttgtaatgct cgatcatgct
     2401 aatgcttcaa aatctgtgaa gtttattgac cgaccaaaag gtgataaagt atttgttaaa
     2461 aagactgaag aattaaaaac agactttgca catcaggaag ctgaatatga tgattatttg
     2521 cgcgaattta tgttaccgca taaaatgagc acattaggtt caggtattgc cgtaggtgat
     2581 gtaaatggcg atggagctga tgattattat attggtggtg gcgcaggtca atcaggagca
     2641 ttatatattc aaaatgcaaa tggttcattt gaaaaatctg catggcagca aacagcagct
     2701 gatggtgcag ctagtgaaga tgctggtgca ttatttttcg atgcagatgg tgatggtgat
     2761 aacgacttat atgtagcatc cggcagcaac gaatttgcag caaacgatgc taaatatgcg
     2821 gacagattat atatgaatga tggtaaaggt aatttctcca aatcaggcga tgctttacct
     2881 ggttttaaat caagtagttc atctgtaaat ggtgccgact ttgataaaga tggtgatatc
     2941 gatttattta tcggtggccg tcaggttccg ggacgttacc ttgctcctgc ttccagttac
     3001 attctgcaaa acaatggtgg caaatttact gatgttactg ctcaggttgc tccaatgttg
     3061 aaagactgtg gtatgattac ctctggttta tggaccgact tcgataatga taataacatc
     3121 gacttaatct taacaggcga ttggatgccg gttactttcc tgaaaaataa aggtggcaag
     3181 tttactgacg taactaaaaa taccggttta acaaatgcga ccggttggtg gcaaagtatt
     3241 accggggccg atatggataa tgatggtgac atggattatg tagtcggaaa tttcggaact
     3301 aaccgacgct ataaaaacac tacagcagtt tccgatggcc acgctttacc attagaagcc
     3361 ttcctttacg atttcgataa aaatggtcag gaagattaca ttatgtgtta ttatcagcac
     3421 gataaattat tcccggttaa aactcgtgaa cgtatgttgg aacaaattcc aactatggct
     3481 gagcaatatc caacatgggg cgcttatggt gatgctaccg ttcaggatat gtttggtgat
     3541 gatttagaaa aatctattca tcgcaccgcg tatattttcc atacctctgt tttaatgaat
     3601 gaaggtaatg gcaaattcac gatgaaattt ttaccaaacg aagctcagat ttcatctaca
     3661 tttggtatgg taactgacga cttcaatgaa gatggtatcc aggatatatt attacatggt
     3721 aacttctaca atacggaaat tgaaattacc cgtcacgatg ccggaacagg attattatta
     3781 tatggtaaag gcgatggtac atttgcccca caacgtggtt atataaccgg atttagaagt
     3841 gatggtgatt cgaaagcatt agctacaatt atcgacagta aatcaaaaca accagtttat
     3901 ctgcttggta attcaaatgg aaaaatggaa agttttggat tgataaaaac cactcaaata
     3961 attacattgg ccgcaaacga tgcttatgca attgtttcat ataaagatgg taaaaaatcg
     4021 aaagtggaat tgtatagtgg ttcaggatat ttgagtcaaa gtgctaaaat ggtgcgtgta
     4081 acacctaatg tagctcaaat agatgtatat gattacaaag gccaaatgcg cactgtattt
     4141 ccggctgcgt tgagtgctaa ataatatttt acggtttaat ttaaaaaggc tgtttcttaa
     4201 ttgaaacagc ctttttttat tttaatgtat ccggtggatt tatcatcctg aaatgcccgt
     4261 ttaattttgc tttctctttt atttctgcaa tttgtttgac agatggcagt atttttttta
     4321 tgtgttccac aattaatttc tgccttgatg tttcccttgg atgacgcaat aattcatatt
     4381 cttccataaa ttcaaatcca acataatgac cgagtttaaa agaattcaat acgaaatcgg
     4441 gtttataaat atcttgtttg atatccaata gcgaaaataa ggtatctgtt agttcctcca
     4501 attcagctag taagcggcta tgtcggtctt caatattttg tattacagaa acaatcgcac
     4561 cgtcgtataa tttaccgggt acttctttca cggtttctaa aatttgaatc acatgcacac
     4621 cttttacctt cacatccatt tcaccgtttg ggtattcctt ttgaatttta accaactcca
     4681 tttctgttcc ataatctaac atgcggttat tttccacaac aggaatacca aacatttttt
     4741 cttcctccaa acattcacgt atcaattgcc tgtacctggg ttcaaaaatg tgcaaattaa
     4801 ttacttcacc gggaaaggca acaaggttta atggaaatat cggaataaat ttttgcatcc
     4861 ttaacactac gaattatttt attgaaacca actattttgt aaaaagttta catccgctca
     4921 cctgccctaa ctgcaacttg caatttattg ctcaatggtg gaatgggaca agaatacgaa
     4981 tgattataat aacaataagg ctgataagct tcattaaagt caataattat tttttgggta
     5041 ttaattgctt taaaatccag atatctgcct cccccatatg tttcgttgct gttggtcaaa
     5101 tcaaaaaagg gtacaaaata aataacctca ccctgtgctt ttaaactttg attttcgtat
     5161 acagccaatt tacaagctac tccttgtaat gtaaatatca aatcgccaat atgtttatat
     5221 ttatcagcaa ttttgcctgt agttgaaaat tcgaatactt catttgagtt cactttttca
     5281 aaatcggcaa tcactttgaa attggtatct acaggaaaat aatttacacc ttcaaagttg
     5341 ggtttaagcg aatcgctaac agggcctgaa gcactcataa aatattgatt agtagcggct
     5401 ctgcttttac gaatggcgtc aaaatacgca ctatcgttct gtatcactac ctcattactt
     5461 tgctgtttgc aagccgataa tacaacagca attaccagta aacacaacag gcgatttttt
     5521 ttcatgtata agttatgata taattgatta cttaaattaa cgttgtttac accttcaaat
     5581 gtatagtaaa ttgtttcaaa aatagtaagt ttacataaca agctgatttt taattaaaaa
     5641 atacttatgc tggatattga aaaatttcgc agttattgcc tggcgaaaaa aggtgttact
     5701 gaagaattcc catttgggga agacacgctg gtgtttaaag taatgggcaa aatgtttgcc
     5761 ctaaccggtt tagacgctga aagttttaga atcaatttga aatgtgacag cgagcagata
     5821 acttatttac gggctaaata tccggcggta caacccggtt atcacatgaa caaacaacat
     5881 tggaatactg taatggttga tggcagtatt cctgaaaagg aagtaaaagc catgattgac
     5941 cattcatatg atgaagtagt aaaagggatg acaaaaaaac aacggcaaga gctcgaaaac
     6001 ctgtaaattt gagattttaa tcaacgatat gcttccaccg gaaatactat ttgaggataa
     6061 tcatttaata gcgataaata aacgccccgg agaaatttcg caaggtgata aaacgaatga
     6121 tgttccattg gttgaaatag taaaacaata tctcaaagaa aaataccaga aaccgggtaa
     6181 tgtatatgcc ggactcatac accgaattga cagacccgtt agtggtgtat taatttttgc
     6241 caaaaccgga aaagccggag aacgtatgtc gaaaattgtt cgtgatagag atttcaaaaa
     6301 aacatattgg gcgattgtaa aaaataaacc ggaacaagaa tcggcaaccc tcgttcatca
     6361 tttattaaaa aatgaacgta tgaataaaag ttttgcttac accgaaaaaa aaggtgatag
     6421 caaggaagcc attttacatt atcaattaat tgcctccagc gaccgttatt ttttattgga
     6481 agtagaccca caaacgggca gacatcatca aatacgtgca caattagcca ccattggaag
     6541 tccaataaaa ggggatttaa aatatggttt cgaccgttct aataaagatg gtagtgtaag
     6601 tttacatgcc cgacagcttg aatttattca ccccataaaa aacgaaccgg taattatcac
     6661 agctaatcct cctgttgatc cggcatggaa cgcatctatg gaaattttag ggcacaaaca
     6721 taacccgcaa tcaattccgg gttatcataa aatatttttc ttataattta ttcatcatca
     6781 cttccaccta atttcgcatc agggaacata aaattaatgc ctaatgaaaa tccaatgtag
     6841 gcattgtgcg aagccaaata aatattttca ccctgagccg gttgaaggtt acgcgtttct
     6901 ttagcattga tatcaaagaa accataataa cctctaaatg aaggtgaaaa atataattta
     6961 tcgtttacgt aaatatcagc tccaacgtct aataacaaac caacatccag tttttggaaa
     7021 taatcagttc cgtttacgct ggcattacct ctaaaatctg gcacaccgga ttgaggtgca
     7081 gtattaaccg gtttttgatc atccggatca atattcataa tatcagctgt ataggtttgt
     7141 tcagcactta ataaataacc aaattgtaac ccacctaaca attgatattt aatcgcatct
     7201 ttggtatcac cttccatata tttaaataat acaggaacct gtaaataatt taaatcaaca
     7261 taacggtagg taagtacttt agtttgtaaa tccggttttg aagtatcctc agttggcccg
     7321 aaatctttca taatatcaga ataatcctga cccatttgag cgtaattaaa ttctacctga
     7381 ataccgaaat tatcttccca gttataccct aaagttaagc ctccagttgg accgaatttg
     7441 tattcgtaat ccatttctga atactcatag ttgttctggt ttaaaatcca gatactattc
     7501 atttgtccat gaaatccaaa atgaatacct tgttgagcct gagctgccgc gccgacaaac
     7561 aagataatcg agagcgtgag taacgttttt ttcatacctt ttagttaatt aattcacgaa
     7621 tatacgacct tttcacaaat gcgcaactta gtatcaacat ttgtgaagaa aattgaataa
     7681 aaaacataca gtgtttgcta aaccatccgg acacaaatta ttagattttt ctcattctgt
     7741 gttgctacaa tccatgtaaa ataagccttc caacaaaaag taaggttaat aattttaaac
     7801 caaccttact tttacctaag ttcaaatctt ttgtctttca acgaaatagt gtgaaattat
     7861 ggttaaatta atcctaaatt ccatgtaaca gaaatcgata aaattcacca atttcaagtc
     7921 aaatcttcct aaaatccata aaagcagcac gtcacaaata ccccaattca gaaatgtgag
     7981 acaaatgtta tcttcgcagc cgtgagtaaa ctgaaaacag caatacgata ttgcaactac
     8041 ctgctaagcg ctaaaaccag gtacgaagtc cattccccat tcgtttacag ctttattaat
     8101 gatattttac gcgaccgacg ccattattat gcgttcgacg agatagaaca tc
//
LOCUS       DAORKW010000105         8049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_254_length_8049_cov_5.042782, whole genome shotgun sequence.
ACCESSION   DAORKW010000105 DAORKW010000000
VERSION     DAORKW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_254_length_8049_cov_5.042782"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLB46_05935"
     CDS             <1..978
                     /locus_tag="PLB46_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF76090.1"
                     /translation="NCAGPGGGGSGGLLWMSTGTLPAGITLISDGGNSGVTVGEGAAS
                     PCFNSTNFAQDGADGGFLNNLVLPAPTELYIELTVDMIPDDAVVCAGNELFMSVIATG
                     TGSLNYQWNDPTTTNTPDLIIVPPYDFTYAVTVTDDLGCQLIGFVEVDVIDSVAITAY
                     PDTTLVMGNFMTLYTNLDDPYTILWTPDYNISDITDPNPLINPYETTTYCVSATHPTG
                     CVSTDCVTIIVAAEVALPNAFTPNGDGVNDIFRVPPTANLCEEVQYFKVFNRWGDPIY
                     DYFKDFDRGGWDGNDYFGRSQEIGTYVYVVKMLCDGVSETYSGTVHLLR"
     gene            complement(1029..1688)
                     /locus_tag="PLB46_05940"
     CDS             complement(1029..1688)
                     /locus_tag="PLB46_05940"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HRF76091.1"
                     /translation="MKTLLLITGILIAGLFLQCDGNSTLFYNARKATADSLQKADSIN
                     TSLLISKAGYAKTYCIKNKFDTSICLLINMREHSGNFRFYVWDFETNQPIDSGLVSHG
                     CGDNPWGMDASSNNPIFSNIPESHTSSLGKYKVGKRDISQWGVGIKYYLHGLESTNSQ
                     AYERQIVFHSWNAIPDVATYPIGAPEGWGCPAISNNFFNRVDNLLMAKNKPVLLWIFN
                     E"
     gene            complement(1751..2383)
                     /locus_tag="PLB46_05945"
     CDS             complement(1751..2383)
                     /locus_tag="PLB46_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76092.1"
                     /translation="MRIFVLLAITIIALSSCNNSSKHKDANGALLVDFLTYQDGSPIS
                     FAPMKHEDSAFVESFQAEICLFEGEPYTGKVALYDSEKLKLEGQLKDGKFDGKWSFYY
                     PSGVVQIEGTYTNGIETGMWTTYYRKDYPKMMKYYDEKGYLLMRKEYFDNGKVKNYQN
                     VKCPQFGNLERRVQFNYKGEVEYIDAERELGRLEPAAINKLLQDNGLMVK"
     gene            complement(2398..>2688)
                     /locus_tag="PLB46_05950"
     CDS             complement(2398..>2688)
                     /locus_tag="PLB46_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76093.1"
                     /translation="VDDSVRRGRDGDGEKRPRRRREPPQLTLSGHTHGFQMGIETPAI
                     RFSPSQFIYDQWAGLYQKNNVAIYVNRGLGTVGYPGRIGIWPEITQITLRKK"
     assembly_gap    2689..2698
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2699..3768)
                     /locus_tag="PLB46_05955"
     CDS             complement(<2699..3768)
                     /locus_tag="PLB46_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF76094.1"
                     /translation="MDVQAIITRLTVTGIAYFVSDIFIHSAIKKAFNTNPKVIRYYWI
                     SNITLFILASVGFVLLQYIQPTLARINLWFFGIFISLLFSKLVVLIILFGEIIIRFIG
                     SLFTYNKRKKHQQPFMLSRRTFIARMSLLIGAIPFAGFAYGIFKGRYNFTIHKLEVAF
                     DDLPESFDGFTLTQLSDLHIGSWDKSAENELEYLVQTVQELKSDVVFFTGDIVNSRAD
                     EMDGWYTTFNKIAAPFGVFSILGNHDYGDYIKWKTEADREENLKNVKNIHPQLGFNLL
                     LNENVALEKNGEKIYVIGVENWGKGDFPKIGDLDIASQGVDDQSFRILLSHDPSHWDA
                     KVLEKSNPPQLTTTGFTTTLPS"
     gene            complement(3786..4259)
                     /locus_tag="PLB46_05960"
     CDS             complement(3786..4259)
                     /locus_tag="PLB46_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76095.1"
                     /translation="MKKVLDSVKNVFDETTGLLGEGYQSATGLLKETLSNFYDVKNLT
                     SEKLADMANDLIALSPIIEKTGFRTKEINVGVSIPPRIVFHFEKFADVSKADIDIILK
                     ENEDKTLLKVIVTTLVAADDFQKKLTLGSFKFNEIDIEVGVPPEVNVKLVNASAH"
     gene            complement(4274..6166)
                     /locus_tag="PLB46_05965"
     CDS             complement(4274..6166)
                     /locus_tag="PLB46_05965"
                     /inference="COORDINATES: protein motif:HMM:NF018000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76096.1"
                     /translation="MSTFSKKLRYAFDNTMSKGPIALIIWLAIISILVIILAAFVLMV
                     TGINQPEEEGLNFMEAAWQSLMRTLDAGTMGGDAGWPFRIIMLLVTIGGIFIVSTLIG
                     VLSSGIEGKLEELRKGRSEVMENDYTLILGWSSKVFTIISELVLANENQKKPRIVILA
                     GIDKVEMEDAIQSNIGDTKNTKIICRSGDTTNPLDLLIVNPKKAKSIIILGKDENESD
                     FEIIKTILAITNNPDRKPEPYHIIAEMRETQNFDVARMIAKDEAELILTDDIVARIMV
                     QTSRQSGLSVVYTELMDYGGDEIYYKNEPLLYGKTYAEALMAYDTSCIIGLKQNGLSR
                     INPPMDTIISNTDEIIAISADDDTIKLNQKTQFDIQQQAITSSLPPAVKKVERIILLG
                     WNRRVIRVIKELNNYVGKGSTIHVVANVELPQELAPNNAAQYENIQIEFTGAETADRN
                     VLESLQVFNADYVMLFSYELENDIQRSDSITLITLLHLRDMSEKAGKDLNLVSEMLDL
                     KNRELAEVTNADDFIVSDKMLSLLITQVSENKHLMRVFEDILDEEGSEIYMKPISDYI
                     DISNPINFYTITTSGIQKGHTVIGYRLMADEHNAAKSYGIRLNPDKPEMIKFSADDKL
                     IVLADD"
     gene            6402..6929
                     /locus_tag="PLB46_05970"
     CDS             6402..6929
                     /locus_tag="PLB46_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF76097.1"
                     /translation="MINWSKPQEGDYIPYQINYISLVPDGDLIEIMRSQIDATAAYLA
                     ELTEEQIQYRYAPGKWTIKEVLQHMIDCERIMCYRALRIARKDTTPLPGFEENDYVTN
                     ANVDERDFIDMVREYIAVRAGTIYMFRSFNNTILLNRGTANNNIMNVNAFAYVIAGHE
                     LHHMKIIKERYVSEK"
     gene            6997..7641
                     /locus_tag="PLB46_05975"
     CDS             6997..7641
                     /locus_tag="PLB46_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRF76098.1"
                     /translation="MSNSSEYKQLLQNNANWVEQKLATDPTYFEMLSQGQKPPFLYIG
                     CSDSRLPIDVFTGSIPGQIFIHRNVANQVFLNDMNLLTVLEFSVKTLKVQHIIVCGHY
                     GCGGVQAAYTGTTLNMVENWTMNIRDIAMENKKELDAIPDLTERLNKLSELNVIRQLR
                     QLCKTSVMHDAFRKNEYPKLHGWVLDISHGLIKELPIPIDEWKEYGLLPSSYEP"
     gene            complement(7629..>8049)
                     /locus_tag="PLB46_05980"
     CDS             complement(7629..>8049)
                     /locus_tag="PLB46_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRF76099.1"
                     /translation="YDALDDMLPMEKLNLYMQNRVIEIAPLAFMRGRTLDHAFIILDE
                     AQNATDAQIKMFLTRIGPSAKCIITGDLTQIDLPRNQSSGLYTSLRILKDIDGIGTIF
                     LNENDVVRHRLVKQIIKAYDKEKTRIENRVSDEHQGS"
BASE COUNT         2430 a         1483 c         1411 g         2715 t
ORIGIN      
        1 aattgtgcag gacccggtgg tggcggctca ggtggcttgt tgtggatgag caccggtaca
       61 ttacctgccg gaattacact tatttctgat ggcggtaact ctggagttac tgttggagaa
      121 ggcgcggcat ccccgtgttt taactcgaca aattttgctc aagatggcgc tgatggtgga
      181 tttttaaata acctggtcct tccggcaccc actgaattgt atattgaatt aaccgtagat
      241 atgataccgg atgatgcagt tgtttgcgcc ggtaatgaac tttttatgag tgttattgct
      301 acaggaacag gttcgcttaa ttaccaatgg aatgacccaa caactactaa tacgcctgac
      361 cttattattg ttcctccata cgatttcacc tatgcagtaa ctgtgacaga tgatttaggc
      421 tgtcagttga ttggttttgt agaagtggat gtgattgatt cagtagcaat tacagcgtat
      481 cctgatacca cgttagtgat gggtaatttc atgaccttat atactaattt agacgaccct
      541 tatactatat tatggacacc ggattataat atttctgata ttacggaccc taacccacta
      601 attaatcctt acgaaactac tacgtattgt gtttctgcaa cgcatcctac aggttgtgtt
      661 tcaactgact gtgttactat tattgtagct gcagaggttg ctttacccaa tgcctttaca
      721 ccaaatggtg atggtgtgaa tgatattttt agagttccac caacagccaa cctttgcgag
      781 gaggttcaat actttaaagt gtttaacaga tggggtgatc cgatatatga ctattttaaa
      841 gattttgaca gaggtggttg ggacggtaat gattattttg gacgctcgca ggaaatcggt
      901 acttatgtat atgtagttaa aatgttgtgc gatggtgttt ctgaaactta ttcaggcact
      961 gttcatttat taagataatt tatgctattt gaaaaaggaa cggatgactt attagttatc
     1021 cgtttttttt attcgttaaa tatccatagt aatacaggct tatttttcgc cattaataag
     1081 ttgtcaaccc ggttaaaaaa attgtttgaa atagcagggc aaccccatcc ttccggtgct
     1141 ccgattggat aagttgcaac atctggtatt gcgttccaag aatgaaatac aatttgcctt
     1201 tcatatgcct gactgttagt tgattcaaga ccatgtaaat agtattttat accaacaccc
     1261 cactgactga tatcacgttt gccaactttg tatttcccta atgatgaagt atgactttca
     1321 ggaatattag aaaatatggg attgttggaa ctagcatcca tcccccaagg attatcaccg
     1381 caaccatgac taaccagtcc ggaatcaatt ggctgattgg tttcaaaatc ccatacataa
     1441 aatcgaaaat tacctgaatg ttcgcgcatg ttaatcagca gacaaattga agtatcaaat
     1501 ttatttttaa tacaataagt ttttgcgtaa cctgcttttg aaattaataa actcgtatta
     1561 atactatctg ctttttgcaa actatctgca gttgctttgc gggcattata aaagagtgtt
     1621 gaatttccat cacattggag gaaaagtccg gctattaaaa tgccggtgat aagcagaagg
     1681 gttttcattc agtaagtgtg tactgaacaa cgataaacac tatttattta ttattaaaaa
     1741 cgaaaattgc ttatttaacc attaatccat tatcttgaag gagtttattg attgcagcag
     1801 gttctagtcg gcccaattcg cgttcagcat caatatattc aacttcacct ttataattga
     1861 attgcactct tctttccaaa ttaccaaatt gagggcattt cacattctga taatttttta
     1921 ctttaccgtt atcaaaatat tctttgcgca tcagtaaata tcctttttca tcataatatt
     1981 tcatcatttt aggatagtct ttacgataat aagtcgtcca cattcctgtc tctataccat
     2041 ttgtatatgt tccttctatt tgaactacac cacttggata gtaaaaggac catttgccat
     2101 caaatttgcc gtctttcaac tgcccttcca gttttaattt ttcgctatca taaagtgcta
     2161 ctttacctgt gtatggttca ccttcaaaca aacaaatttc tgcttgaaaa ctttccacaa
     2221 atgcactgtc ttcatgtttc attggagcaa aggaaattgg agagccatcc tgataagtta
     2281 aaaaatcaac taataaggcg ccgtttgcat ccttgtgttt ggaagaattg ttacacgatg
     2341 ataatgctat tatggtgata gccaacaaaa caaatattct catggtataa agttaattta
     2401 tttttttcga agtgtaattt gtgttatttc aggccaaata ccgattctac cagggtatcc
     2461 tacagtacct aaaccgcggt taacataaat agccacgttg tttttttgat aaagacctgc
     2521 ccattgatcg taaataaact gagaaggaga aaatcttatt gcaggagttt caatacccat
     2581 ttgaaatcca tgtgtatgtc cgctcaaagt taattgtggc ggctcccggc gtcttctcgg
     2641 tctcttctca ccatccccgt cacgcccgcg tctcacacta tcatccacnn nnnnnnnntg
     2701 ggatggaagt gtggtggtga atccggttgt cgttaattgt ggcgggtttg atttttccaa
     2761 tacttttgca tcccaatgcg atggatcatg tgataataaa atacggaacg attggtcatc
     2821 cactccttgt gatgcaatat ctaaatcgcc tatttttggg aaatcgcctt taccccagtt
     2881 ttcaacgcca attacatata ttttttcacc atttttttcc aatgccacgt tttcattcaa
     2941 caataaatta aaccctaatt gaggatgaat attttttacg ttctttaaat tctcttcacg
     3001 atcggcttct gttttccatt taatgtaatc gccataatca tgatttccaa gtatagagaa
     3061 aacaccaaac ggcgctgcaa ttttgttgaa agtagtatac caaccatcca tttcatcggc
     3121 gcggctgttt actatatctc cggtaaaaaa aacgacatca gattttaatt cctgaacggt
     3181 ttgaacaagg tattctaact cgttttcagc acttttatcc caagaaccaa tgtgtaagtc
     3241 gcttaattgc gttaatgtaa atccatcaaa tgattccggt aaatcgtcaa atgctacttc
     3301 cagtttatgt atggtgaagt tataacgacc tttaaatatg ccataagcaa atcctgcaaa
     3361 gggaatagct ccaatcagca aagacatacg agcaataaat gttcgtctgc ttaacataaa
     3421 tggttgttga tgttttttgc gtttgttgta agtaaacaag cttccaataa aacgtataat
     3481 gatttctcca aacaaaatta ttaataccac aagtttggaa aacaacaacg agataaatat
     3541 gccaaaaaac cagagattga ttcgtgctaa tgtgggctga atgtattgca acaaaacaaa
     3601 tcccactgat gccaaaataa acaatgtaat attgcttatc caataatacc ggattacttt
     3661 gggattggta ttaaatgcct tctttatggc actgtgaata aaaatgtcag atacgaaata
     3721 tgctattcct gtaactgtaa gtcgtgtaat tattgcctga acatccatat gcaaagcgta
     3781 acagtttagt gtgccgaagc gttcaccaat ttaacattta cttcaggagg tacccccacc
     3841 tcaatatcaa tttcgttgaa tttaaaactg cccaatgtga gttttttctg gaaatcgtca
     3901 gcggcaacca gtgtggttac aatcactttg agtaatgttt tgtcttcatt ttcttttagg
     3961 ataatatcaa tatctgcttt acttacgtca gcaaattttt caaaatgaaa tacaattctt
     4021 ggtggaatgc taactccgac attaatttct ttggtgcgaa acccggtttt ttcaataatc
     4081 ggtgataggg caatgaggtc gttcgccata tctgccaatt tttcgctggt taggttttta
     4141 acatcataaa aattacttaa agtttctttc agcaatcctg tagccgattg ataaccttct
     4201 cctaacagcc ctgttgtttc gtcaaaaacg ttttttaccg aatccagtac ttttttcata
     4261 attatcggca aagttaatca tctgccaaaa caattaattt atcatcagct gaaaacttta
     4321 tcatttccgg tttatcgggg tttaaccgaa taccatacga tttagctgca ttgtgttcat
     4381 ctgccattaa tcgataacca atcacggtgt gacccttttg aatgccggat gtagtgattg
     4441 tataaaaatt aatagggttg ctgatgtcaa tataatcgga aatcggtttc atgtaaattt
     4501 cactaccctc ttcatctaaa atatcctcaa aaacacgcat taaatgtttg ttttcactaa
     4561 cctgggttat taataaactt aacattttat cgcttacaat aaagtcatcg gcattagtaa
     4621 cctcggcgag ttcgcgattt tttaaatcaa gcatttcact taccaagttc aaatcttttc
     4681 ctgctttttc actcatgtcg cgtaagtgta ataaagtgat taatgtaatg gagtcgctgc
     4741 gctgaatatc attttctaat tcataactga aaagcataac ataatcggca ttaaatacct
     4801 gcagcgattc caatacattt ctgtcagcag tttcagcgcc ggtaaactct atttggatat
     4861 tttcgtattg tgcagcgtta tttggtgcta gttcctgagg taattccaca ttcgcaacta
     4921 catgaatggt ggaacctttc cccacgtagt tatttaattc tttaattaca cgaataactc
     4981 ttctgttcca acctaataaa attattcgtt ccactttttt cactgcaggt gggagtgaag
     5041 aagtaattgc ctgctgttga atatcaaatt gtgttttttg attgagtttg atggtatcat
     5101 catcagcact gatggcaatt atttcatctg tattactaat aattgtatcc attggcggat
     5161 taattcgact taaaccattt tgttttaaac cgataataca acttgtatca tatgccatta
     5221 atgcttcggc ataagttttt ccgtataata atggttcgtt tttgtaataa atttcatcgc
     5281 caccgtaatc cattaattca gtatacacta cactcaatcc gctttggcgc gaagtttgaa
     5341 ccataatgcg tgcaacaata tcatcagtta atataagttc ggcttcatct ttagcaatca
     5401 tccgcgctac atcaaaattt tgtgtttctc gcatttctgc aataatatga tagggttccg
     5461 gtttgcggtc cggattattt gttattgcca atattgtttt aataatttcg aagtcacttt
     5521 cattttcatc tttgcccaaa ataataattg attttgcttt tttgggattt acaattaata
     5581 aatccagtgg gttagtagta tctccacttc tgcaaattat tttagtattt ttagtatcac
     5641 cgatattgga ctgaatagca tcttccattt caactttatc gataccggct aaaattacta
     5701 tacgtggttt tttttgattt tcgttagcca acactaattc gctgataatg gtaaatactt
     5761 ttgaactcca tcctaatatg agggtgtaat cattttccat tacttcacta cgtccttttc
     5821 ttaattcttc caatttacct tctataccgc tagacaaaac accaattaat gtgcttacaa
     5881 taaaaatacc tccaatggta accaacaaca tgattattct aaacggccaa ccagcgtcac
     5941 cacccatggt tccggcatct aatgtgcgca ttaaactctg ccaagcagcc tccataaaat
     6001 ttaatccttc ttcttcaggt tgatttatgc cggttaccat caatacaaat gcagccaaaa
     6061 taatgaccaa tatggaaata attgccaacc agataattaa tgcaatgggg cctttgctca
     6121 tagtattatc aaaggcataa cggagttttt tggaaaaggt actcatgatg tatgtttgaa
     6181 gtaaaaatat caattttttg tgaatggcct tattatttag gggtttaatc aataactgca
     6241 accaattaat aaataacagc aggtttagcc aataacatat tattgaaaat gcaatatgtt
     6301 catgttttgt ttggtctttt taagtcagtt taatacgcta taatttgttc caacatttgt
     6361 ttggatacct gaagtttagg acatttgtaa aaaaattaga gatgataaac tggagcaaac
     6421 cacaagaagg tgattatata ccatatcaaa taaattatat cagtcttgtt cccgatggcg
     6481 acttaattga aataatgcgc agtcagattg atgcgacggc agcatattta gcagagctta
     6541 cggaggagca aattcagtat cggtatgcac cgggaaagtg gacgattaaa gaagtgttac
     6601 aacatatgat tgattgcgaa cgaataatgt gttatcgcgc attacgtatt gccagaaaag
     6661 atacaacacc acttccggga tttgaagaaa acgattatgt tactaatgcc aatgttgacg
     6721 aacgtgattt tattgatatg gtgagggagt atattgccgt gcgcgcaggt actatttaca
     6781 tgtttagaag ttttaataat accattttat taaacagagg aactgcgaat aataatatta
     6841 tgaatgtaaa tgcatttgcg tatgtaattg caggacatga attacatcat atgaaaatta
     6901 ttaaggagcg gtatgtttcc gaaaaatgag ttaaacattt ttaaaaacta attgttaggg
     6961 taggaattta aatttttgcc acttatcttt gatactatga gcaattcgtc ggaatacaaa
     7021 caattattac aaaataatgc caattgggtg gagcaaaaac ttgctactga cccaacatat
     7081 tttgaaatgt tgtcacaagg gcaaaaaccg ccttttcttt acattgggtg cagtgacagc
     7141 cgtttgccga ttgacgtgtt tacgggctcc attccgggac aaattttcat ccataggaac
     7201 gttgccaatc aggtgttttt gaatgatatg aatttgttaa cggtgttgga attttcagtt
     7261 aaaactttga aagtgcaaca tattattgtt tgcggtcatt acggatgcgg aggtgttcaa
     7321 gcggcatata caggaactac attgaatatg gtggaaaact ggaccatgaa tatccgtgat
     7381 attgccatgg aaaataaaaa agaattagat gctattcccg atttaacaga gcgtctaaat
     7441 aaactcagcg aattaaatgt aatccgtcag ttgcgacaat tatgtaaaac atcggtaatg
     7501 catgatgcat tccgcaaaaa tgaatatccc aaattacatg gctgggtact tgatatttct
     7561 cacggattaa ttaaagaatt accaatccca attgatgaat ggaaagaata tggtttatta
     7621 cctagcagtt atgaaccttg atgttcatcg ctaacgcggt tttctattct ggttttttct
     7681 ttatcgtaag cttttataat ttgttttacc agtcggtgac gaacaacatc attttcgttg
     7741 aggaaaatag tgcctattcc atcaatgtct tttaatattc ttaatgaagt gtacagccct
     7801 gagctttgat ttctaggtaa atctatttgt gttaaatcac ctgtaatgat acattttgca
     7861 ctaggaccaa ttctggttaa aaacattttt atttgtgcat cagtagcatt ttgagcttca
     7921 tctaaaataa taaatgcatg atctaaagtt ctaccgcgca taaacgccaa aggagcaatt
     7981 tcaattaccc tgttttgcat atacaggttg agtttttcca taggcagcat gtcgtccaat
     8041 gcatcgtat
//
LOCUS       DAORKW010000106         8042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_255_length_8042_cov_4.775009, whole genome shotgun sequence.
ACCESSION   DAORKW010000106 DAORKW010000000
VERSION     DAORKW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_255_length_8042_cov_4.775009"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..391
                     /gene="trxA"
                     /locus_tag="PLB46_05985"
     CDS             86..391
                     /gene="trxA"
                     /locus_tag="PLB46_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRF76100.1"
                     /translation="MAQRSSFTDIIQSEQPVLIDFFAEWCGPCKSLAPILKQFAGNYA
                     DKVRVIKVDVDKNPLVAAQYQIQGVPTMILFKQGKPVWRQSGVVSLSMLTQEINKHI"
     gene            404..934
                     /locus_tag="PLB46_05990"
     CDS             404..934
                     /locus_tag="PLB46_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF76101.1"
                     /translation="MTILLVIIGGGLAGYLYWRVFGCAFGICPLSQNKYMSTLIGVLL
                     GLMVFTSSSCSAQTNEEHREPNPTTIQSSIVNEDVKPEVFASKIGTENTVLIDVRTPE
                     EYAEGHMPNALNINFNAPDFAEQMEKMDKSKIYLVYCRSGARSGRAADLMSTSGFNTI
                     YTLQGGVLGWNQPLVK"
     gene            complement(1163..2005)
                     /locus_tag="PLB46_05995"
     CDS             complement(1163..2005)
                     /locus_tag="PLB46_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRF76102.1"
                     /translation="MKKLLLLIALSGIIQFAQAQTKSCCSAPDQFASLSSDIAFVNKH
                     ENPVPFTFISEMGVAVTVMCNDGTTANGYALKSTNSSNKFLFVFHEWWGLNDYIKQTS
                     EQLYKDLNAEVNIIAIDLYDGKVAAVRDSAKAYMNALSPERAKVIIDGFLTHVGDSAQ
                     ITTIGWCMGGGYSMQASLQMQQQAIGCVMYYGMPESNTERLKTLQADVLMIWPNQDKW
                     INKEVVDTFKANMVSLDKKLFVEEYNADHAFANPSNPKFDNEAATNANNKALIFLKSK
                     FGIN"
     gene            complement(2060..3226)
                     /locus_tag="PLB46_06000"
     CDS             complement(2060..3226)
                     /locus_tag="PLB46_06000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRF76103.1"
                     /translation="MQVILQKGKGRRVENGHPWVYGNEIDKIEGNPQTGDIVEVYNFR
                     NEFIGKGYINQLSQITVRLLTRDKSVNIDKSFFRERLLSCKAYRTTLNYIENYRLVFG
                     EADFLPALVIDKFNDVYVIQTLSAGMDRWKKIITEILMEDFGASCVYERNDVPVRKLE
                     GLEETKGFLSPETATQFVINEKGVNFFLDIENGQKTGFFLDQKENRMAIQQIVKDKTV
                     LDCFTYTGSFALFAAYYGAKHVTALDISADAIALANKNIGLNDFTNITTICENAFDVL
                     PVWAKEGKQFDVVILDPPAFTKNRKGIENAVKGYKEINLRGMKLVKPGGFLVTYSCSH
                     FMDVNLFYDTIASAATDANRTVREVQFLTQSKDHPIVWGVEETNYLKGLVLQIL"
     gene            3311..4129
                     /locus_tag="PLB46_06005"
     CDS             3311..4129
                     /locus_tag="PLB46_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76104.1"
                     /translation="MYIGIGAVLFTYQGYFLLHQIPKLDVLLLLGGAATMFVYLLSRV
                     AAVSRIKEYAPETRWDFFTRNLFLMQVLTVITFLITVVTYLMLPRSIQILLFFPGLIS
                     LLYGVPIRFGKTQFRLRDIGVTKIFMIAFVWAFIGSFLPAANSALPMFSGEVILLFFA
                     HYLFIFGITLPFDIKDLKIDAQHQVKTIPAIIGIQKSYNLSFLSLLLSTILYVVMQQL
                     TTEQDFSLPLCIAMLLSSWSIYLSRKKADNYLYFGLIDGMLIIQSLLVIGFAFN"
     gene            complement(4126..4545)
                     /locus_tag="PLB46_06010"
     CDS             complement(4126..4545)
                     /locus_tag="PLB46_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76105.1"
                     /translation="MAFMHHFNYKQVLGLLIIVLLSSAAINRKNKAASNAPSEDVKEM
                     DAMLLTDLKQQEITIDEHTQCLMECASIQESELRALFDINNVNYSKCDAGNCHLTSYT
                     IEGKLDNGKAVSFKLDSGEEGNQLRALNITEADCNCS"
     gene            4595..5566
                     /gene="bioB"
                     /locus_tag="PLB46_06015"
     CDS             4595..5566
                     /gene="bioB"
                     /locus_tag="PLB46_06015"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRF76106.1"
                     /translation="MGEIRNDWTKEEVAEIFNLPIMELMYRASVVHRKFQATGEVQVC
                     TLLSVKTGGCPEDCAYCPQASRYHTEVERHNLLGYDEVLTKALIAKENGSTRFCMGAA
                     WRNIKDNEDFDNVLEMVKGVSNIGLEVCCTLGMLTEDQAKKLKDAGLYAYNHNLDTGE
                     EYYEEIISTRNYGDRLKTIENVSNADISVCSGGIIGLGESQADRVAMLHTLATMKKHP
                     ESVPVNALVPVKGTPLENNTRVSVWEMIRMIATARIIMPRAMVRLSAGRNEMSVEEQA
                     LCFMAGANSIFAGEKLLTTPNPDVNEDKAMFELLGLKPREAYKEVLV"
     gene            5647..6795
                     /locus_tag="PLB46_06020"
     CDS             5647..6795
                     /locus_tag="PLB46_06020"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HRF76107.1"
                     /translation="MSIQERLNNAILKRRQENALRELTVTNNLVDFCSNDYLGFAQNA
                     EIHNYLLQELPKLRKIGSTGSRLLKGNYSLIEKVENEIANFHQAPAALFFNSGFEANA
                     GLIASVVSRHDTIIYDELCHASLREGIRLSAATAFSFTHNDTAALTNKLQKAKGEKFI
                     VIESVYSMDGDCCPLQEIVTIAKTFNAAIILDEAHATGVIGKKGEGLAQHLALQNDIF
                     ARVHTFGKAIGNNGAVVMGDEVLRQYLINFCKPFIYTTAPNYLQVLAVKKAYTLLAGQ
                     HESIATLNKNIQHFKFLISRFKHIQMLPSESAIFSIIIPGNDSVKLAAKYIQNAGYDV
                     RPVLSPTVPEGKERLRICLHSFNTELEIERLVKLLEQITGIIQSDATV"
     gene            6804..>8042
                     /locus_tag="PLB46_06025"
     CDS             6804..>8042
                     /locus_tag="PLB46_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF76108.1"
                     /translation="MEKIIDLIVTPEIAADMSAVKKVIAKQVGLPDTLFCEIIKRSID
                     ARSKQVKINLRVKYSTEKMPVQHWDIPQYPNVKNGKPVVIIGAGPAGLFAALELLEQG
                     LKPIIFERGKDVRERRFDLAAINKQGIVNGESNYCFGEGGAGTYSDGKLYTRSDKRGN
                     IRKVLELFVRFGASPEILIEAHPHIGTNKLPKIIEKMRTTIIEAGGEVHFNHKVTNFF
                     TAFNKITGVELNGTTTFDCDKIILATGHSARDIFELLHRNNILIEAKPFALGVRIEHP
                     QQVIDKMQYHCEVRGPLLPPSSYSLVQQVQGRGVYSFCMCPGGIIAPCATAPGEVVTN
                     GWSPSKRNNPFANSGFVVSITLDDTQAFKKMGPLAAMAFQQQTEQLCFAAAGGKQQAP
                     AQRLVDFTSNTVSNSLPDCSY"
BASE COUNT         2472 a         1396 c         1582 g         2592 t
ORIGIN      
        1 atttttgtca tttttaaaaa gctgtcgtga caaaagtcac taatttggat agaacgaata
       61 acgaattttg tgtgttataa aaggtatggc acaaagaagt agttttacgg atattattca
      121 gtcggaacaa cctgttctta tcgacttttt tgctgaatgg tgtgggccgt gtaaaagtct
      181 cgcgccaatt ctgaaacaat ttgccggaaa ttatgcggac aaagtgcgtg tgataaaagt
      241 agatgtagat aaaaatcctt tagtcgcggc acagtatcaa attcaaggtg taccaaccat
      301 gattttattt aaacaaggta aacctgtctg gcgacagtct ggtgttgtgt ctttgtcgat
      361 gttaacgcag gaaataaata aacatattta aaacagcatt tttatgacaa tattactggt
      421 aattattggt ggcggacttg ccggctactt atattggaga gtttttggat gtgcattcgg
      481 gatttgtcct ttgagccaaa ataaatatat gagtacatta atcggtgttt tattgggatt
      541 gatggtattt acatcctcaa gttgttcagc gcaaacaaat gaggaacatc gtgaaccaaa
      601 tccaactaca attcaatcat caattgtaaa tgaggatgta aagcctgaag tttttgcatc
      661 taaaatcgga actgaaaata cggtattaat tgatgtgaga acacctgagg aatatgcgga
      721 aggacatatg ccaaatgcat tgaatattaa ttttaatgcg cctgattttg ctgaacaaat
      781 ggaaaaaatg gataagtcga aaatatattt agtgtattgc agaagtgggg caagaagtgg
      841 tcgtgcggcg gatttaatgt cgacttcagg atttaatact atttatacat tgcaaggggg
      901 agtattaggc tggaatcagc cactggttaa gtaaattaca ctatagtttt gtttcacgcg
      961 aaggggtttc acgcggaggg ccggagaact ttattgtgtt atgtaagatg tggattgttt
     1021 ttgagagcgc ggagctggta aaccaattaa ttattattct ccgctaactc ccttctccgc
     1081 gtgaaacatt tcgtgaatta aaaattttac ttatttcacg cggaggggcg gagagtgaag
     1141 tatttattag ctataatatc ggttaattaa taccgaattt ggattttaaa aatatgagcg
     1201 ctttattgtt cgcgttggta gccgcttcat tatcaaattt gggattactt ggatttgcaa
     1261 atgcatgatc ggcattgtat tcttctacaa acagtttttt atctagcgaa accatattcg
     1321 ccttgaatgt atccaccact tctttattaa tccatttatc ctgattaggc caaatcatta
     1381 atacatcagc ttgtaaagtt tttaaccgct ccgtatttga ttcaggcatg ccataataca
     1441 tcacacatcc tattgcctgt tgttgcattt gtaaacttgc ctgcatggaa taaccaccac
     1501 ccatacacca gcctatagtt gtaatttgtg cactatcgcc aacatgcgta agaaatccat
     1561 caataatcac cttggctctt tcgggagata atgcattcat ataagctttt gcactatcac
     1621 gcactgccgc taccttgcca tcgtataaat caatagcaat aatatttact tcagcattta
     1681 agtccttata caattgctca cttgtttgtt tgatataatc atttaatccc caccattcat
     1741 gaaatacaaa caaaaattta ttggaagaat tagttgattt cagcgcataa ccattggccg
     1801 ttgtgccatc gttgcacatt acagttacag caacacccat ttcgctgata aaagtgaaag
     1861 gaaccggatt ttcgtgtttg ttaacgaacg ctatatcact cgacaaagaa gcaaattggt
     1921 caggagctga gcagcatgat tttgtttgag cctgcgcaaa ttgaattatg cctgaaaggg
     1981 caattagtag taataatttt ttcataatga taaatttaag ttacaattca atattgttaa
     2041 agcaaatact gtaaaacagt tataatatct gtaacaccag tccttttaaa tagttcgttt
     2101 cctctacgcc ccacacaatt ggatggtctt tcgattgtgt taaaaattgc acttcacgaa
     2161 ccgtacgatt agcatctgtg gctgctgagg ctatggtgtc ataaaataaa tttacatcca
     2221 taaaatgaga acaactgtag gtgactaaaa acccaccggg ttttacgagt ttcataccac
     2281 ggaggtttat ttctttatat cctttaaccg cattttcaat cccttttctg tttttggtaa
     2341 atgcaggcgg gtcgagaatt accacgtcaa attgtttccc ttcctttgcc caaaccggta
     2401 aaacatcaaa agcattttcg caaatagtag taatattagt aaaatcgttt aaaccgatat
     2461 ttttattagc caatgctata gcatctgcag aaatatctaa ggctgttaca tgtttggctc
     2521 cataataagc tgcaaataat gcaaacgaac ctgtataggt aaaacaatct aataccgttt
     2581 tgtcttttac tatttgttga atagccatcc ggttttcttt ttggtcgagg aaaaatcctg
     2641 ttttctgacc attttctata tctagaaaaa aattaacgcc tttttcgttg atgacgaatt
     2701 gtgttgcagt ttcgggagat aaaaatcctt ttgtttcttc caatccttcc aatttacgga
     2761 caggcacatc gttgcgttca tatacgcaag acgcaccaaa atcttccatt aaaatttccg
     2821 taatgatttt tttccaacga tccattcccg cacttaatgt ttgtatgaca tacacatcat
     2881 taaatttatc aataaccaat gcgggtaaaa aatctgcttc accaaatact aatcgataat
     2941 tttcaatata atttaaagtt gtacggtagg ctttgcaaga taataatctc tctcgaaaaa
     3001 atgatttatc aatgtttaca gatttatcac gggtaagtaa acgaacagtt atttgggaaa
     3061 gttgattgat atatcctttg ccaataaatt catttctgaa attatacact tcaacaatat
     3121 ctcccgtttg gggattgcct tcaattttat caatttcgtt gccgtatacc catgggtgac
     3181 cattttcaac acggcgccct ttgccttttt gtaaaattac ctgcaccttt taattaattt
     3241 tgtgtgaaaa tactataaaa tcctgaagca aatactacat catatcatta actggctgtt
     3301 atacagtaat ttgtatattg gaattggtgc tgtattattt acctaccagg gctattttct
     3361 tttacatcaa atacccaaat tggatgtgtt gttgctatta ggtggggctg ctaccatgtt
     3421 tgtatattta cttagtcgag ttgcagctgt ttcgcgtatt aaagaatatg cacctgaaac
     3481 acgatgggat ttttttacgc gcaatttatt cctcatgcaa gtgctgactg taattacatt
     3541 tttaattaca gttgttacct atttaatgtt gccgaggtcg atacaaatat tattgttttt
     3601 ccctggatta atttctttat tgtatggtgt tccaattcgt tttggtaaaa cacaatttcg
     3661 acttcgtgat attggtgtga ctaaaatttt tatgatagca tttgtgtggg catttatagg
     3721 tagtttttta ccggctgcaa atagtgcact gccaatgttt tcaggggaag tgatactgtt
     3781 attttttgcc cattacttat ttatttttgg cattacattg ccattcgata ttaaggattt
     3841 aaaaatagat gcacaacatc aagtgaaaac aattcctgcc attataggca ttcaaaaaag
     3901 ttataattta tcgtttttgt ctttgctgtt gagcaccata ttatatgttg taatgcaaca
     3961 gcttactacc gaacaagatt ttagtctgcc attatgtata gccatgctgt taagcagttg
     4021 gagtatttac ctctcccgta aaaaagcaga taattattta tattttggat tgatagatgg
     4081 tatgctcatc attcaatcac ttttggtgat agggtttgca ttcaattaac tacaattgca
     4141 gtcagcttcg gtaatgttta aagcgcgcaa ttgattgcct tcttcacccg aatctaattt
     4201 aaaactaaca gccttgccat tgtccagttt tccttcaatg gtatatgaag ttaaatggca
     4261 gtttcctgca tcacatttac tataatttac attgttgata tcaaataatg cgcgaagttc
     4321 tgattcctga attgaagcac attccattaa acattgagta tgctcatcaa tggtaatttc
     4381 ctgctgcttc aaatcggtca ggagcatggc atccatttct tttacatcct cagagggagc
     4441 attacttgcc gctttgtttt tgcggttgat tgccgcgctg cttaacaata ctataatcag
     4501 taaaccaagt acttgtttat agttaaagtg atgcataaat gccataatgt aaaaatttgc
     4561 tccaaaatta cgaattttgc ccaaaaatat gtcaatggga gagatacgca acgactggac
     4621 taaagaggaa gtggccgaaa tttttaacct gccgataatg gaactgatgt atcgcgcaag
     4681 tgttgtacat cgcaagtttc aggctacagg tgaggttcag gtttgtacct tgttatcggt
     4741 gaaaaccggt ggttgccctg aagattgcgc atattgccct caggcttccc gctaccatac
     4801 ggaagtggaa cgccacaatt tgttaggata tgatgaggtg ctcaccaagg cgttgatagc
     4861 caaagaaaat ggctctacac gtttttgtat gggtgctgcc tggagaaata taaaggataa
     4921 tgaggatttc gacaatgtgc tcgaaatggt gaaaggggtt tcaaatattg ggttggaagt
     4981 atgttgtacc ttaggtatgt taaccgaaga tcaggctaaa aagttaaaag atgccggttt
     5041 atatgcctac aatcataatt tggataccgg tgaggaatac tatgaagaaa ttatttcaac
     5101 acgcaactat ggcgatcgtt taaaaaccat tgaaaatgtg tccaatgcag atatttcagt
     5161 ttgtagcggt ggcataatcg gattaggtga atcgcaagcc gacagagttg caatgttgca
     5221 tacgctggct acgatgaaaa aacatcctga atcggttccg gtaaatgcat tggtacctgt
     5281 taaaggaaca cctttagaaa ataatacacg cgtgagcgtt tgggaaatga taagaatgat
     5341 tgcaacggca agaataatta tgccgagagc aatggttcga ttaagtgctg gaagaaatga
     5401 aatgagtgtg gaagaacagg ccttgtgttt tatggccggg gccaattcta tttttgcagg
     5461 tgaaaaatta ttaaccactc caaaccctga tgttaacgaa gataaagcga tgttcgaatt
     5521 attgggatta aaaccacgtg aagcttataa agaggtgttg gtgtaaaccc ggtgcaacgt
     5581 tgtttgtctc attttttatt tcaatttagt tgtttattta ttcgcagcaa ttaaccgtaa
     5641 aaatatatgt cgatacaaga gcggttgaat aatgcaattt taaaacgccg gcaggaaaat
     5701 gcattgcgcg aattaactgt caccaataat ttggtagact tctgttcaaa cgactacctg
     5761 ggttttgccc aaaatgccga aatacataat tatttattgc aggaattgcc caagttgcga
     5821 aaaattggtt ctaccggttc gcgattatta aaaggcaatt attcattgat tgaaaaagta
     5881 gaaaatgaaa tcgctaattt tcatcaggca cctgctgctt tgttttttaa tagtgggttt
     5941 gaagcgaatg ccggattaat tgcatcagtt gtatctcgtc acgatacgat aatttatgat
     6001 gaactttgtc atgcttcatt gcgcgaaggc attcgactat ctgctgctac tgctttctca
     6061 tttacgcata acgatactgc tgcactgacc aataaattac agaaagcaaa aggcgaaaaa
     6121 tttattgtaa ttgaatcggt ttattccatg gatggagatt gttgtccgct acaagaaatt
     6181 gtgacaatcg ccaaaacatt taacgcagct attattttag atgaagcgca tgccaccggt
     6241 gtaataggaa aaaaaggaga aggtttagcg caacatttag cattacaaaa tgatattttt
     6301 gcacgtgtac atacatttgg aaaagcaata ggtaataatg gcgcagttgt aatgggagat
     6361 gaggttttga gacaatacct aataaatttt tgcaaacctt ttatttatac cactgcgcct
     6421 aattatttgc aggttttagc agtgaaaaaa gcctatacgt tattagctgg tcaacacgag
     6481 tcgatagcta cattaaataa aaatatccaa cattttaaat tcctgatttc ccgatttaaa
     6541 catattcaaa tgttgccgag tgaatctgca atatttagca ttattattcc cggaaacgat
     6601 tcggtgaaat tagctgcaaa atatatacaa aacgcaggtt atgatgttag accggtatta
     6661 tcacccacag tgcctgaagg gaaagaaaga ttgcgtattt gtttacacag ttttaatacc
     6721 gaattggaaa ttgagcgatt agtaaaatta ttggaacaaa ttacagggat aattcaatct
     6781 gacgctaccg tataatcaac gccatggaaa aaataatcga cttaattgta acacctgaaa
     6841 ttgctgctga tatgtccgca gtaaaaaaag tgattgcaaa acaagttggt ttgcccgata
     6901 cgcttttttg tgaaattatt aaacgttcaa ttgatgcccg ctcgaaacag gtgaaaataa
     6961 atttgcgggt gaaatatagc acagaaaaaa tgcctgtgca acattgggat attccgcaat
     7021 atccgaatgt gaaaaatggc aaacctgttg ttataattgg tgcgggccct gccggtttat
     7081 ttgcagcttt ggagttgtta gagcaaggtt taaaacctat catttttgag cgcgggaagg
     7141 acgtgcggga gcgccgtttc gacttagcgg ccatcaacaa acaaggtata gttaacggag
     7201 aaagtaatta ttgttttgga gaaggaggag caggcactta cagcgatgga aaattatata
     7261 cgcgttctga taaacggggc aatattcgta aagtattgga attgtttgtt cgcttcggtg
     7321 caagtcctga aatattaatt gaagcacatc cacatatcgg gacaaataaa ttaccaaaaa
     7381 ttattgaaaa aatgcgtact accattattg aggccggtgg tgaagtgcat tttaatcata
     7441 aggtgactaa tttttttact gcttttaata aaattaccgg tgttgaatta aatggcacaa
     7501 ctacatttga ttgtgataaa ataatattag caacaggaca ttctgctcgt gatatttttg
     7561 aattattaca tcgcaataat attttaattg aagcaaaacc ttttgcttta ggtgtgcgca
     7621 ttgaacatcc gcaacaagtg atagataaaa tgcaatatca ttgtgaagtg cgtggtccgc
     7681 ttttaccacc ttcatcttat agtttagtgc aacaagtgca ggggagaggc gtatattcat
     7741 tttgtatgtg ccccggtggt attattgcac catgcgctac tgctccgggt gaagtggtta
     7801 ctaatggttg gagcccaagc aaacgcaata atccttttgc taactccgga tttgttgtaa
     7861 gtattacatt ggacgatacc caagcattca aaaaaatggg accgcttgcc gctatggcat
     7921 ttcagcagca gactgaacaa ttatgttttg ccgctgccgg tggaaaacag caagctcctg
     7981 ctcagcgctt agtagatttt acatcgaata ctgtttctaa ttccttgcct gattgttcct
     8041 at
//
LOCUS       DAORKW010000107         8040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_256_length_8040_cov_4.396493, whole genome shotgun sequence.
ACCESSION   DAORKW010000107 DAORKW010000000
VERSION     DAORKW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_256_length_8040_cov_4.396493"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PLB46_06030"
     CDS             <1..914
                     /locus_tag="PLB46_06030"
                     /inference="COORDINATES: protein motif:HMM:NF036911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-glutamine glutaminase family protein"
                     /protein_id="HRF76109.1"
                     /translation="LVILLVLLSSITPGCQKSIDILEETPTLQLTDEYRLMTPAYYRE
                     SEDGASIQVRFYESPRIYNLSKSHKDFDAIKALIDQSRDAKTPMRVVEGNKVMPFISL
                     TAAEKPSAQEILDAATKYDVTPEPDIFKKFVPNMTILNGIFDYCASQGCATGTSTIDY
                     CIPFQYVVDGCYARAHKMRQIMLEEYNVSCEKVFSYEDVTGFLAVDAGDCCVYWWYHV
                     APLVTVKGADGSYTKWVIDPSMFNEPVSIETWTGAQENLSCSPAANWGYYEITPGKIY
                     GPGGSTDNSYISTNWTLQAYANLETCD"
     gene            complement(989..5101)
                     /locus_tag="PLB46_06035"
     CDS             complement(989..5101)
                     /locus_tag="PLB46_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF76110.1"
                     /translation="MIRKMYIFGMFVSILSLFQQTQAQTAFTAGNIVIVRVGDGSTSL
                     INTGNAVFLDEYTTTGSLVQSIALPTVIAGSNKRLILSGTATSEGALSRSADGSALVL
                     TGYDATPGGATSLSGTSAASVNRTVGLIYADGTFNTSTGLTDFASANNPRSATSTNGT
                     DLWMTGGAGGIRYATLGATTSNDLSSATLANVRVVQIFDNQLYFSTASGTTYRVGSVG
                     TGLPTSGLQTLTHLTGIPASTGSPYGYFMADLDGAPGIDVIYVADDGANALRKYSLVA
                     GTWTLNGTIGVDADDYRGITGSVSGTTVTLYCTIKGGSGATGGGQIVTLTDASGYNGA
                     FAGVPTVLFTAATNTSIRGIAMAPEVTCTPVLWYADADGDNYGDLATSQSSCTMPSGY
                     VADSTDCNDGDGAIYPGAAELCNSIDDNCDGNTDEEFINPIISFASSELVAGLTGPSS
                     SQSPYLTPLKPGVQFTSIFTAGDVFGGYTASGLMDGIGAYDNGDSTFTVLISHEINNT
                     LGVVRDHGFIGTFVSKWIINKSDLSVISGEDLMQNAFVWNNLLNMYEPASAAFSRFCS
                     SDLPAQTAFYNAVSGNGSMEKIFMNGEESGNEGRQFAHIVSGAEAGNSYELPALGKAG
                     WENSVASYYAQDKTVVVELDDNTTNGQVYVYVGTKTNTGLEIEKAGLTNGILYGITVD
                     GMSTESSLSIPAANTAFSLTSLGSVTGLSGATLNTNSITAGVTNFLRPEDGAWDPQNP
                     AIFYFVTTNSFTAPSRLWKLEFTDINNPELGGTITALLDGTEGPKMMDNMTVDNAGNI
                     IIQEDPGNQKYLARIWEYRIATDELIELAQHDENRFISGAPSYLTQDEESSGVIDISS
                     ILGPGMLLLDDQAHYSIPGAVVEGGQLLVMYNPETATGVYTATDTVRVNTNTACTATG
                     VVLGTPMTSDNCVVASVTNDAPTVYPIGNTTVTWTVTDGSGNSTTGTQVVIVTDEVLP
                     TITAPAAVIVSADSSCTASEVSLGTPITADNCGVATVTNNAPATYPLGATTVTWTVTD
                     VNGNFATVAQTVTVTGTAATYYADVDGDTYGNSSDSIVACVIPSGYVVDNTDCNDSDI
                     AINPGATEICNGIDDNCDGNIDDNLIIATVTPVGPTSFCRGNSVQLDANTGVGYTYQW
                     KKNGTNIPGATAASYTANKPGNYSVLVTIPGGCSDLSDAVTVTVLASPAASITNVTGW
                     TDLCTGSPIKLKANGGATLTYQWYRGASMIAGATSNTYNVTLSGNYSVEVTNTVTGCS
                     TKSAKYPITQTCKEGEDVIIESSSFNVYPNPTNGSFTIDMTINASQEATADILLYNMI
                     GEVVYQSSIEVNNNTIFANVTPDNNLSEGLYIVKVVVGNTIFEKSIAVVK"
     gene            complement(5436..6080)
                     /locus_tag="PLB46_06040"
     CDS             complement(5436..6080)
                     /locus_tag="PLB46_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRF76111.1"
                     /translation="MKSNYILYTLISCFVLFSYTASAQQFRYGANIAPAASWWIVEGD
                     LYITSGNSIGFQVGGTIDLTLGESERFAIQSGLNFTSAPGKFEQNPESLSFAGKAWDY
                     SVKTIDLPILLRLRSDELGKTVLFAQYGLTLGFTMASEVKIRDGKNGGGEFDYESLNT
                     SLTMGTGIEYALNDNMDLMLTVFFQNGTKNMLISNANDDNMFPQQLGLRAGILF"
     gene            complement(6109..6774)
                     /locus_tag="PLB46_06045"
     CDS             complement(6109..6774)
                     /locus_tag="PLB46_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF76112.1"
                     /translation="MKKTLLLLFSLVTLAVQAQQFRYGFSIAPAQNILSFDTDLYSEP
                     TAYRGFQYGLLLDQTFGKGEHIALTFGLNLNYTNSGLTSVVEGQGDDLKEWAVRGRYL
                     EIPLTLRLRTGQIGKFIFYGEGGASYGHGLRAVGDYTVNGVRRDSDFDYYDKGNGNGI
                     TYYEKNASIVMGLGAEIAITETASILIGFFHQKGLLSVYEDNNDESILKLNQTGIKIA
                     GLF"
     gene            complement(7025..7453)
                     /locus_tag="PLB46_06050"
     CDS             complement(7025..7453)
                     /locus_tag="PLB46_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76113.1"
                     /translation="MYKSSTTQIIALATTIMLLTACGNKKTTSPEVPENTDTLVQTTI
                     TQPEVTDNTTETPPANTIAEKTETPVIKADVSGKGTIVQKGDIWVIRTTGSEGTDYLP
                     SNLTKAFQVDGKTVTFEANLDEIPPGVRMAGRPITLVSIK"
     gene            7834..>8040
                     /locus_tag="PLB46_06055"
     CDS             7834..>8040
                     /locus_tag="PLB46_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRF76114.1"
                     /translation="MPTIQQLVRNGRVAIKAASKSRALDACPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNQTEIIAYTPG"
BASE COUNT         2559 a         1546 c         1399 g         2536 t
ORIGIN      
        1 aattagttat cctgttggtg ctcctcagct ccatcacccc gggatgtcaa aaaagtattg
       61 acattttaga agaaacaccc acattacaat taacggatga gtatcgttta atgacaccgg
      121 cctattatcg ggagtctgaa gatggcgcat ctatacaggt gcgtttttat gaatcgccgc
      181 gtatatataa tttatccaaa agtcataaag attttgatgc cataaaagca ttaattgacc
      241 aaagtcgtga tgctaaaaca cctatgcggg ttgtagaagg aaataaagtg atgccgttta
      301 tttcattaac cgctgctgaa aaaccttctg ctcaggaaat attagatgca gccacaaaat
      361 acgatgtcac ccctgaacct gatattttta aaaaatttgt gcctaatatg acaatattaa
      421 acggcatatt tgattattgt gcatcacaag gttgtgcaac aggtacttct actatcgatt
      481 attgtattcc atttcaatat gtagttgatg gttgttatgc acgtgcacat aaaatgcgtc
      541 aaatcatgtt agaagaatat aatgtgagtt gcgaaaaagt ttttagctac gaagatgtca
      601 ccggattttt agcagtagat gcaggcgatt gttgtgtata ttggtggtac catgttgcac
      661 cactggttac agtaaaaggt gctgatggct catatacaaa atgggtaatt gaccctagta
      721 tgtttaatga accggttagt attgaaactt ggacaggcgc acaagaaaat ttgagttgtt
      781 ctcctgcagc aaactggggt tattatgaaa ttacaccagg caaaatatat ggtccgggtg
      841 gttcaacaga taattcatat atttctacta actggacttt acaagcttat gccaatttgg
      901 aaacttgtga ttaaataatt tataatttac agtcaataaa aaatccgcaa acatttacgt
      961 ttgcggattt tttatttaat gtttattgtt attttacaac tgcaattgat ttttcaaaaa
     1021 ttgtattacc aactacaact tttactatat ataaaccttc tgaaagatta ttatcagggg
     1081 taacattagc aaaaatagta ttgttattta cttctattga agattgataa acaacttctc
     1141 caatcatgtt atataacagg atatcagcag ttgcttcttg cgaagcgttt attgtcatat
     1201 caattgtaaa tgaaccattg gtagggttag gatatacatt gaatgatgag ctttcaataa
     1261 ttacatcttc accttcttta caagtttgag taatcggata cttagcagat tttgtactgc
     1321 aaccggttac tgtattggta acttcaacgg aataattacc ggacaatgta acgttataag
     1381 tatttgaagt tgcaccagct atcatcgatg caccacgata ccattggtag gttaatgttg
     1441 caccaccatt cgcttttaat ttaattgggc ttccggtgca aagatctgtc catcctgtta
     1501 cattagtaat tgaagcagca gggctagcta atactgttac agtcacagca tcagacaaat
     1561 cagaacatcc gcctggaata gtaactaata ctgaataatt acctggtttg ttagctgtat
     1621 aagatgcagc agttgcgcca ggaatattag tgccattttt cttccattgg taagtataac
     1681 ctacgccggt attagcatct aattgaactg aattacctct gcagaatgaa gtaggaccaa
     1741 caggtgttac agttgcgata attaagttgt catcaatatt gccatcacaa ttatcatcaa
     1801 ttccgttgca tatttcagtt gcacctggat taattgcaat gtcactatca ttacaatcgg
     1861 tattatcaac tacataacct gaaggaatta cacaagctac aattgaatca gatgaattac
     1921 cataagtatc accatcaaca tcagcataat aagtagcagc agttcctgta acagttactg
     1981 tttgagcaac agtagcaaaa ttaccgttaa catcggttac agtccatgtt actgtagtag
     2041 cacctaatgg ataagttgca ggagcattat ttgttacagt tgcaacacca cagttatcag
     2101 cagttatagg agtacctaaa ctaacttcag aagctgtgca agatgaatca gcacttacaa
     2161 ttactgcagc aggagcagtt atggtaggaa gaacttcatc tgtaacaatt acaacttggg
     2221 ttcctgttgt tgaatttcct gaaccgtcag ttacagtcca tgttactgtt gtattgccaa
     2281 taggatatac tgttggagca tcattcgtta cacttgcaac aacacagtta tcggaagtca
     2341 taggagttcc taaaacaaca cctgttgctg tgcatgcagt attagtattt acacgaacag
     2401 tatctgttgc agtatatact ccagttgcag tttcaggatt atacatcact aataactgtc
     2461 caccttctac aactgctcca ggaattgaat aatgagcctg atcgtcaagt aataacatac
     2521 cgggtcctaa aattgaagaa atatcaataa cacctgaaga ttcttcatct tgagttaagt
     2581 aagaaggggc accagaaata aatctgtttt cgtcgtgttg agcaagttca attaactcgt
     2641 cggtagcaat acgatattcc caaatacgag ctaaatattt ttgattaccc gggtcttcct
     2701 gaataataat attacctgca ttatctacag tcatattgtc catcattttt ggaccttctg
     2761 tgccatctaa taatgcagta attgttccac ctaattctgg gttattgata tctgtaaatt
     2821 ccaatttcca taaacggcta ggagctgtaa atgaattggt tgttacaaaa taaaatatag
     2881 caggattttg tggatcccat gcaccgtctt caggacgtaa aaaattagta acaccggcgg
     2941 taattgagtt tgtgtttaat gttgcaccag ataatccggt aacagaacca agtgatgtca
     3001 aagaaaatgc agtatttgca gctgggatgc ttaatgatga ttcagtagac ataccatcta
     3061 ctgtgatgcc atataaaata ccatttgtta aacccgcttt ttcaatttca agacctgtgt
     3121 tagttttagt tccaacatat acataaacct ggccgtttgt tgtattatca tctaattcta
     3181 ctacaacggt tttatcctgc gcataatagc tggctactga attttcccaa cctgctttcc
     3241 caagtgccgg taattcatat gaatttcctg cttctgcacc tgatacgata tgtgcaaatt
     3301 gtcttccttc attacctgac tcttcaccat tcataaagat tttttccatt gaaccatttc
     3361 cacttactgc attataaaaa gcagtttgag ctggaaggtc agatgaacaa aaacgactaa
     3421 aagctgctga tgcaggttca tacatgttta ataaattgtt ccaaacaaat gcattttgca
     3481 ttaagtcttc accagaaatt actgataaat ctgatttatt aataatccat tttgaaacaa
     3541 atgttccaat aaaaccatgg tcgcgtacaa caccaagggt attgttgatc tcatggctga
     3601 ttagtactgt aaatgtacta tcgccattat cataagcacc aataccatcc attaaacctg
     3661 aagcagtata tccaccaaaa acatcacctg cagtaaaaat tgacgtaaac tgaacaccag
     3721 gttttaacgg tgttaaatat ggagattgtg aacttgaagg tcctgttaaa ccggctacta
     3781 attcgcttga tgcgaaagaa ataatcggat taataaattc ttcatctgta tttccatcac
     3841 agttgtcatc aattgaatta cataattctg cagcaccagg ataaattgca ccatcaccat
     3901 cgttacaatc agtactatca gctacataac ctgaaggcat tgtgcaagat gattgtgaag
     3961 ttgctaaatc accatagtta tcaccatcag catcagcata ccataaaacc ggtgtgcaag
     4021 ttacttccgg tgccatagca ataccgcgaa ttgatgtatt tgtagcagca gtaaaaagta
     4081 cagttggaac tccggcaaat gcaccattgt aacctgaagc atctgttaag gttacaattt
     4141 gtccaccacc agttgcgcct gaaccacctt taattgtgca atataatgtt acagttgttc
     4201 cactcacaga accggtaatt ccacgataat catcagcatc aacaccaatt gttccgttta
     4261 aagtccaagt acctgcaact aacgaatatt ttcttaatgc atttgcgcca tcatcagcaa
     4321 cataaataac atctattccc ggagcaccat ctaaatcagc catgaaataa ccatatggag
     4381 aacctgttga agcaggaata cctgttaaat gagttaatgt ttgtaaaccg ctggttggca
     4441 atcctgttcc aacagaacca acacgatagg ttgttccgct tgcagttgaa aaatacaatt
     4501 gattatcgaa aatttgtact acacgaacat ttgctaatgt tgcactagat aaatcatttg
     4561 aagttgttgc gcctaaagta gcataacgaa tgccacctgc tccacctgtc atccataaat
     4621 cagttccatt agtggatgta gctgaacgtg gattatttgc cgatgcgaaa tcagttaatc
     4681 ctgtagaagt attaaaggtt ccatcagcat aaattaatcc aactgtgcgg ttcacagaag
     4741 ctgctgatgt tccacttaaa gaggttgcgc cccccggtgt tgcatcataa cctgttaaaa
     4801 ctaatgctga accatcagca gatctggata atgcaccctc tgaagtagcg gtgccactta
     4861 atattaaacg tttattggat ccagcaatta cagtaggtaa tgcaattgat tgaaccaatg
     4921 aacctgtggt ggtatactca tcaaggaata cagcgttacc tgtatttatt aatgacgtac
     4981 taccatcacc aacgcgaaca attacaatat tgcccgcagt aaatgccgtt tgcgcttgtg
     5041 tttgttgaaa caaactcaaa atcgaaacga acattccgaa aatgtacatt ttgcgaatca
     5101 taaaattggt tttagtgatt aatttgttaa atgattgcgc aaaattcaag accaactgtt
     5161 gcgttattat taaactacgt gtatttaaag atttaataac aaattcaata atttttttgt
     5221 caggtactaa taaatagtat tactcaggta aattttgttt gaagcaattt cggtgtatta
     5281 ctggtacatc aatttaatgt aatacttttt tatactttgt taatttaatt accggaataa
     5341 tttaataatt gattttcaaa aaggtaatca cactattaaa aataaaaaag gcgattctga
     5401 atgaatcgcc ttaattttta tttaaattat tattattaaa ataatatccc cgcccttaat
     5461 cccaactgtt gcggaaacat attgtcgtca ttcgcatttg aaatcagcat gttttttgta
     5521 ccattttgga aaaacacagt taacattaaa tccatattat catttaatgc atattcaatt
     5581 cctgtaccca tggttaaact cgtatttaaa ctttcataat caaattcgcc accaccattt
     5641 tttccatcgc gaattttcac ttcgcttgcc attgtaaatc caagggttaa accatattgt
     5701 gcaaataaaa ctgtcttgcc taattcatca gaacgcaaac gtaataaaat aggtaagtca
     5761 attgttttta cagaataatc ccaagccttt cctgcaaaac ttaatgattc aggattttgc
     5821 tcgaattttc ccggagcaga tgtaaagttt aatccactct gaatggcaaa tctttcactt
     5881 tctcctaatg ttaaatcaat tgtacctcca acctggaaac caatactgtt tccggaagtg
     5941 atatataaat cgccttctac tatccaccag cttgctgcag gtgcaatgtt agcaccataa
     6001 cgaaattgct gagcactggc cgtgtatgaa aataatacaa agcaactgat gagtgtatat
     6061 aatatataat tagatttcat tttagatgtt gattttgatg tgagttaatt agaataatcc
     6121 ggcaatttta attcctgttt ggttgagctt taatatactt tcatcgttgt tatcttcata
     6181 aacagaaagt aatccctttt gatggaaaaa tcctattaaa atagaagccg tttctgttat
     6241 ggcaatttca gcacctaagc ccataacaat acttgcattt ttttcataat aggtaatgcc
     6301 attaccattg cctttatcgt aataatcaaa atcagaatct cttctaacgc cattcacggt
     6361 gtaatcgcct acagcacgta acccatgacc ataagatgca ccaccttcac cataaaaaat
     6421 aaattttcca atttggcctg tgcgcaaacg aagtgtaagt ggaatttcta aataacgacc
     6481 tctaaccgcc cattctttta aatcgtcacc ttgcccttca accacactgg ttaaaccgct
     6541 attggtataa tttaaattta atccgaaggt taaagcaata tgttcgccct tgccaaaagt
     6601 ttggtcaaga agtaatccat attgaaatcc cctataagca gtaggctcac tatataagtc
     6661 cgtatcaaaa cttaaaatgt tttgtgccgg tgcaatcgaa aaaccgtaac gaaattgctg
     6721 tgcctgaaca gcaagtgtta ccaaagaaaa taataaaagg agtgtttttt tcatattcaa
     6781 ttttaaattg gctgtgaggc gcaaatataa attacaaaat gttacatttt tgtcacaagt
     6841 gggcataatt ctaatacaag ttaaacccag gtatttaaca aaatactgat tgtcaatata
     6901 ttatagctgc ctataaaata aaataggctt aataactatg tcacaattag acatgtattg
     6961 cagttttccg cggttatatg tagatacata tatcggcgga atgtcccaaa tgtgtagtta
     7021 attattattt aatacttacc agcgtaatcg gtcttcctgc catacgaaca ccgggaggaa
     7081 tttcatccag attagcctca aatgttacgg tttttccgtc cacctggaat gctttggtca
     7141 gattagaagg taaataatcg gtgccttctg agcctgttgt tctgattacc catatatcac
     7201 ctttttgaac aatagtgcct ttgccggaaa catcagcttt aattaccggg gtttcggttt
     7261 tttctgcgat agtgttggca ggaggggttt cggtagtatt gtcggtaacc tcaggctgag
     7321 taatagtggt ttgtactaaa gtatccgtat tctctggtac ttcagggctg gtggtttttt
     7381 tgttcccgca agccgttaaa agcataatgg tagtcgctaa agcgataatt tgagtggttg
     7441 atgatttgta catatatata aatatggtgt aaaggtagac aatccattct tctgctcgca
     7501 gtatttagtg tacatctcaa ctaaaatcgc cccatcctgg aggaggttaa agaatttaat
     7561 cttaccctac aaagccctgt tttcatagca ttttggtaag atttgcacat ttagtacaca
     7621 tttttttaag tcttggtttg gattgttcgg tactttgctt acctttgcag tccattttta
     7681 aggccgcgtg tcgaaattaa gggggtaaac accgatagcg tttacaatat tggctcgact
     7741 gcactacttc gcaagatttg tgcacaccga tttgttaatt acctccctaa cttctatctt
     7801 tgcagcccca aaagaaaagt tgaagtttaa caaatgccta caatacaaca attagttcgc
     7861 aatggaagag tagctattaa agctgccagc aagagccgtg cactagatgc atgtcctcaa
     7921 aaacgtggtg tatgcaccag ggtttataca acaactccaa agaagccaaa ctctgcatta
     7981 cgcaaggtgg ctaaagtgcg tttaaccaat caaacagaaa ttatcgccta cactccgggt
//
LOCUS       DAORKW010000108         8013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_257_length_8013_cov_4.761624, whole genome shotgun sequence.
ACCESSION   DAORKW010000108 DAORKW010000000
VERSION     DAORKW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_257_length_8013_cov_4.761624"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLB46_06060"
     CDS             complement(<1..516)
                     /locus_tag="PLB46_06060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF76115.1"
                     /translation="MKSPTYVIVIIIVCGIFSITTMHNTHAWGDDFALYMLHANNIVA
                     GAEYEDTGFIYNDLAPTYSPQSYPPGFPLLLAPVQAIFGINFLADKIYLLCFFLLFLW
                     LVYIWLKDKLDTIYVTGIVFILAFSPFLWQLRESVLSDIPAAVATMAAVLFFEKFVQT
                     KRWDHLLYAGLF"
     gene            complement(518..3352)
                     /locus_tag="PLB46_06065"
     CDS             complement(518..3352)
                     /locus_tag="PLB46_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76116.1"
                     /translation="MKNSLLKLLLIVICNLQFSIAFNQCPGDPGNGLFFCESISRTTL
                     NKYLNKAAAFSYVTNPAYTDSQLDLDIQMLQRLDPRWLGRVAGDWLAGNDDVFEFSRA
                     EYIADRIHTEVSTYIILQAAIYEYIDPKVSLYSDWNILIPSDVLALYGYPAHTGYTGY
                     FEHDNIVYPGTNTPDMNRIEAQMWFYYRATRYIDAGYEALHLGQFMIMNDNDPLNAKW
                     WDLLDKIRTYASTHARRKIVLIDAHVAGSGCEASGIEANDVPYTMLPGGEIPSGQLLF
                     EYTSFGITPDEYFEPIENENNTYDGNDRLVKINYTDCAMYDKGFGGTMPVDWGWSYGQ
                     SHPIPSIAEFDLGGAPEPFLCEGMKTDDYTLCDADGDGSADDTPQWLTWGFGGESSWF
                     GLQSPGYRRYFITYAFNRVKELDEDVYCRVNLRMPMSYSLNPWPGEIYNASNLEYGDE
                     DVIGWLWSNQDQIDFCTKSAVSSEFTHGVTGWQGDKHIRQVVDVSGDGKDDLVGFGES
                     NLIVQTSNGSGFNSPTIWYNNDFTYAFGWSVTNHVRRIADINGDGKLDVIGFDNNGVK
                     VAKSNGSSFNTAYYASVLFGNLDGWNPTKHVRDIGDFTGDGLIDIIGCWENETKVRKS
                     NGSNFITTAPHSDVWSTEFCYSNGWRLEKHLRLIGDVNGDGKDDIVGFKDDDVYVGIS
                     TGTSFVTSIWYEDNFCFNQGWDNNNDYRILADVNGDGKDDIVGFGYWGVQVALSTGTN
                     FSQAEIWIYDFGLDAAVGGWNTALHTRTCADINGDGKSDIVGFGNTGTIVSLSTGKSF
                     SKIGTYIDFNYPVYTNSNNPRILADMDADGRSEIIAYGESQVYLLNCNLGSETFRLSD
                     SNEQENNNNFKIYPNPSSGIINIEFENPVMGELIISDIQGRRIYNETFEMACQFKSIL
                     LNNQTVNGIYFISFITTDQVVHSKKLFLLH"
     gene            complement(3849..5336)
                     /locus_tag="PLB46_06070"
     CDS             complement(3849..5336)
                     /locus_tag="PLB46_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76117.1"
                     /translation="MKTISTIKYYSLNIMLWCSLLLFASSIFGQTGGATFENGPLMNR
                     TRIVPLATTLSNGNIAIFGGRENGFVSSYYTDIYDPVAKTFTELNMTVPRDNAALVTL
                     TDGRYLLVGGGYDWGIPAYATNEIFDPSNNSFTMASSMGYSRMQPAAAQLNNGHVLIV
                     GGWYDDYAAINTEIYNIEDNLYTVSGTLNAPRSQAVVIPTTDGGAIVAGGYTTYGAAM
                     ITTVEYYNVETGSFSILSDEIIPTDPGWTMWGRTRAIVDDKMNNGKYVFLAYRSSPEA
                     EYALVYFDPATKTFEKKFSFVLSEHPTINGGIYDMILDKNGNYVYLLATVSSSDPVEV
                     ALISVDLTNEVVYIPENNYVMPSGEYLFPSLAFIPSTGEILITGVSATPSDYFNATNI
                     TYLLKPEHSVGIEEEMILPNKLTCFPNPSNGKFSVYFDGRVDGRLILTVTNLSGQLMD
                     EIELTGSSQTPLRYDYTNTNLPAGYYQVRLVNNNMDMSVPLIIVK"
     gene            complement(5460..6941)
                     /locus_tag="PLB46_06075"
     CDS             complement(5460..6941)
                     /locus_tag="PLB46_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76118.1"
                     /translation="MKNYIIIFFMAFCFPVISHAQQYDFNKRCIDAYTYIQELRFEKG
                     RQLLELEKKENPKNLIPYYVENYIDFLTTYINENEAEFDKLEPNKTIRLDKLKTGDQQ
                     SPYYLYCQAELLIQWSFSKLKFEEYLSAFTDVRKAYLLLEENEKKFPDFMANKKSLGM
                     LHAIVGAIPDQYKWGVNMLGMDGSIDQGLKELKSIIEYSKTNTFIFKQEVYLYYAFIE
                     LYLGRDATTAWNIVKDLDTKTNLINVFCKASVAMRTGRNDLALQVLISRPTGSEYMQY
                     HYLEFMTGLAKVRKLDAGSSAHFEKFISSYKGKNYIKEAYQKMAWMALINGNKEKYWE
                     YMYYIKSRGADLIDDDKQALYESESKKMPNVTLLKSRLLFDGGYYKDALKQLEGKSAE
                     SFSNDKDKTEFTYRAGRIYDMAGMPDKAKGFYLATIKFGSDLPYYYAAGSAFYLANIY
                     ETEKDYAGATKYYQMCLDMDNKEYQTSLDGLAKAGLNRVKGKK"
     gene            complement(6973..7716)
                     /locus_tag="PLB46_06080"
     CDS             complement(6973..7716)
                     /locus_tag="PLB46_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRF76119.1"
                     /translation="MAAVKEMSVKESLQALWKLQKIHSKIDDITILKGELPMEVSDLE
                     DEIVGLETRMHKLEAELKEIETDISGRKTTIANAKTQIKKYETQQNAVKNSREFDALT
                     KEIELNKLDIELCEKKMKEAGFLKDAKAKAIEDAKEVFENKKKDLKKKKEELERIIEE
                     TEKEEADFAKKADKAEKEIEERLMGAYYRIRSSYRNGLAVVAVSRNSCGGCYAVVPPQ
                     RQAEIKQAKKIIICEHCGRILVDEAAMDA"
     gene            complement(7720..>8013)
                     /locus_tag="PLB46_06085"
     CDS             complement(7720..>8013)
                     /locus_tag="PLB46_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRF76120.1"
                     /translation="VRYTKLPEHPVQKVAICGGSGAFLLKDAMVAGAEVFITADFKYH
                     QFFDANNKIVIADIGHYESEQYTIELISDILMKKFATFAVHLSKINTNPINYL"
BASE COUNT         2517 a         1640 c         1236 g         2620 t
ORIGIN      
        1 aaacagtccc gcatacagta aatggtccca cctttttgtc tgtacaaatt tttcaaaaaa
       61 caatacagca gccatggtag ccacagctgc aggaatgtcg gataaaacac tttctcttaa
      121 ttgccacaaa aatggactaa atgcaagtat aaacacgatg cctgttacat aaatagtatc
      181 aagtttatct ttcagccaaa tatataccag ccataaaaac aataaaaaaa agcataacag
      241 ataaatttta tctgccaaaa aatttattcc aaaaatagct tgcaccggag ctaaaagcaa
      301 tggaaatccc ggcggataac tttgaggcga ataagtaggt gccaaatcat tataaataaa
      361 tcccgtatcc tcatattccg ctcctgcaac aatgttgttg gcatgcaaca tgtataacgc
      421 aaaatcatcg ccccaggcat gggtgttatg catagttgtt atagaaaata ttccgcaaac
      481 tataattatt actattacat aagtaggtga tttcatttta atgtaacaaa aataactttt
      541 ttgaatgcac aacctggtcg gtggtgataa agcttataaa atagataccg ttgacagttt
      601 ggttatttaa taaaatagat ttaaattgac aagccatctc aaaagtttca ttataaattc
      661 ttctgccttg aatatcggat ataattaatt cacccattac agggttttca aattctatat
      721 taattatccc tgaggatgga ttagggtaaa ttttgaaatt attattattt tcttgttcgt
      781 tggaatcact taatctaaaa gtttcagatc ccaaattgca attcaataaa taaacttgag
      841 attctccata agcaattatt tcagatcttc catctgcatc catatcagcg agaatcctag
      901 ggttattcga attagtgtaa acaggataat taaaatctat gtaagtgcca attttagaaa
      961 aacttttacc tgttgaaagt gaaacaatag ttccagtatt accaaaccct acgatatctg
     1021 atttcccatc accgtttata tctgcgcatg tcctagtatg taatgctgta ttccatccac
     1081 caacagccgc atctaatccg aaatcatata tccaaatttc agcctgtgaa aaattagtgc
     1141 ctgttgagag tgctacttgt actccccagt atccaaatcc cacaatatca tctttaccat
     1201 ctccattaac atcagctaat atccgataat cattgttatt atcccatcct tggttgaaac
     1261 aaaaattatc ctcataccaa atgctagtaa caaacgatgt accagtagaa atacctacat
     1321 aaacatcgtc atctttaaag ccaactatat cgtctttgcc atcaccattt acatctccaa
     1381 taagccttaa atgtttttcc agcctccaac cattgctata acaaaattca gttgaccaaa
     1441 catctgaatg tggcgctgtt gttataaaat tagatccatt agatttcctc acttttgtct
     1501 cattttccca acatcctata atgtctatca agccatcacc agtaaaatca cctatatctc
     1561 tgacatgctt tgttgggttc catccgtcta aatttccaaa taatactgaa gcataatatg
     1621 cagtattaaa gctcgaccca ttggattttg ctacttttac accgttatta tcaaaaccta
     1681 taacatctaa tttcccatca ccatttatat cagctatacg tctaacatga ttagtaacag
     1741 accaaccaaa tgcataagta aaatcattat tataccaaat tgtcggggaa ttaaacccac
     1801 ttccattgct tgtttggaca attaagttag attcaccaaa accgactaaa tcatctttcc
     1861 catcgccaga aacatcaaca acttgacgta tgtgtttatc tccttgccaa cctgttacac
     1921 cgtgggtaaa ttctgatgat accgctgact ttgtacaaaa atctatttgg tcttgatttg
     1981 accaaagcca tcctattaca tcttcgtcac catattctaa attacttgca ttataaattt
     2041 cacctggcca cggattaagt gaataactca ttggcattct taaattaact cgacaatata
     2101 catcctcatc cagttcttta actctattaa aagcataagt aatgaagtat cttctgtaac
     2161 ctggggattg taaaccaaac catgatgatt ctccaccaaa accccaagtc aaccactggg
     2221 gtgtatcatc cgctgaacca tcaccatcgg catcacaaag tgtataatca tccgttttca
     2281 taccctcgca taaaaatggt tcaggagctc ctcctaggtc aaattctgct attgaaggaa
     2341 taggatgtga ctgaccataa ctccaacccc aatccacagg cattgttcct ccaaagcctt
     2401 tatcatacat tgcacaatca gtataattga ttttaactaa tctatcatta ccatcataag
     2461 tattattttc attttcaatc ggttcaaagt actcatcagg tgtgattcca aaagaagtgt
     2521 attcgaacaa taactgtcct gagggaattt ctcctccagg taacattgta taaggcacat
     2581 catttgcttc tatacctgag gcttcgcaac cagagcctgc aacatgggca tcgattagta
     2641 caatcttccg tctagcatgg gtacttgcat aagtgcgaat tttatccaaa aggtcccacc
     2701 attttgcatt taatgggtca ttatcgttca taatcataaa ctgtcctaaa tgtaatgctt
     2761 cataaccagc atcaatgtac ctggtggcac ggtaataaaa ccacatctga gcttcaatac
     2821 gattcatatc tggtgtattt gttccaggat aaactatatt atcatgttca aaataaccgg
     2881 tgtagccagt atgcgcagga taaccatata atgctaaaac gtcactaggt attaaaatat
     2941 tccagtctga ataaagacta actttaggat cgatatattc ataaatagca gcttgcaaaa
     3001 taatataagt gctaacttca gtgtgtatcc tgtctgcaat atattcggcc ctactgaatt
     3061 caaaaacatc atcatttccg gctaaccaat cccctgcaac acggcctaac catcgagggt
     3121 ccagcctctg taacatttga atatctaaat ctagctgact gtctgtatat gcaggatttg
     3181 taacataact gaatgctgca gctttattta agtatttatt aagtgttgtt ctagaaatag
     3241 attcacagaa aaataatcca ttacccgggt ctcctggaca ttgattaaaa gcaattgaga
     3301 attgtaaatt acaaattaca attagaagca attttaataa agaatttttc ataattagaa
     3361 agttttaaat aggaataaat attaatgagc tgattgtctt ttaataccaa ctcatacaat
     3421 ctaaaattat gtaaaaaaaa cataaatcca aattttttac acaaaagtta attaaaattg
     3481 acattttcaa atgtaattta ggcttttaaa tggtatcctg taacgctttc aatcggaatg
     3541 cctttaaata atttaaatta acttaatgac gctttatgca gttcggaagt ttcgttgttt
     3601 ttccctttag accaataagt tgtggcatct gctcaacctc cactcgattt ggttaatacc
     3661 caaaatcatc gccccaggca tgggtgttat gcatagtaaa caaactaaaa atagccgtta
     3721 ctaaaataat ggagattaca atatacttgt ggcgatacat aaacttgtat aagccaaaaa
     3781 tacagaacta ttacaaataa aaagaaaggg gctgcagatg caaacccctt ttatgaacga
     3841 taaggaaatt attttacaat aataagcggc acactcatgt ccatattatt gtttactaac
     3901 cgaacctggt aataacctgc aggcagatta gtatttgtat aatcatatct gagcggagtt
     3961 tgtgatgaac ctgtcaattc aatctcatcc atcaactgac cacttaaatt agtaacagtt
     4021 aatatcaatc taccgtctac cctaccatca aaatatacgc tgaatttgcc attagatgga
     4081 ttagggaaac aggttaattt gttaggtaaa atcatttcct cctcaatacc tacactatgt
     4141 tcaggtttta gcaaataggt gatattggta gcattaaaat aatcgcttgg tgttgcactt
     4201 acgcctgtta ttaagatttc gcctgtagaa ggtataaatg ctaacgaagg aaataaatat
     4261 tcaccgcttg gcattacata attattttca ggaatataca ccacttcgtt tgtcaggtca
     4321 acggatatta atgcaacttc aacagggtcg ctgctactaa cagttgcaag taaataaaca
     4381 tagttgccgt ttttgtctaa aatcatatcg taaatgccac cattaatagt tggatgttca
     4441 gacaaaacaa aactgaattt tttctcaaat gttttagtcg ctggatcaaa atatactaat
     4501 gcatattcag cttccggact gcttctataa gccagaaaaa catatttgcc attattcatt
     4561 ttatcgtcaa ctatcgctct tgttctaccc cacattgtcc aacccgggtc ggtaggaata
     4621 atttcatcac ttaaaataga aaaactaccg gtttctacat tataatattc tactgtagta
     4681 atcatagcag caccataagt agtataacct ccggccacta ttgcaccgcc atcagtagtt
     4741 ggtattacca cagcttgcga acgaggtgca tttaatgttc cgcttactgt atacaaatta
     4801 tcctctatat tataaatttc ggtgttgatt gcggcataat catcatacca acctccaact
     4861 attaatacat ggccattatt taattgcgcc gcagcaggtt gcattctgct ataacccatt
     4921 gaactagcca tggtaaatga attattagac gggtcaaata tttcgttggt ggcataagca
     4981 ggaatacccc aatcgtatcc accacctacc aataaatatc taccatcagt tagtgtaact
     5041 agtgcagcat tatcgcgtgg aacagtcata tttaattcag taaatgtttt ggctacaggg
     5101 tcataaatat ctgtgtaata actggaaaca aatccatttt ccctgccacc aaaaatggcg
     5161 atattaccat tacttaatgt tgttgccagc ggcactattc gtgttctgtt catcagtggt
     5221 ccattttcaa atgttgctcc tcctgtttgt ccgaaaatag aagaagcaaa taacaataaa
     5281 ctacaccata acatgatgtt aagtgagtaa tatttaattg tgcttatagt tttcatattt
     5341 accgatttgg ttcaaccaaa tatatccggg aaacacatcc taaacagcaa tcgttatcat
     5401 accaataaac gatataaatc acattttaaa aggcaataca aattggtggt agcgcggaat
     5461 tattttttcc ccttaaccct gttaagtccg gcctttgcta atccatcgag cgatgtttga
     5521 tattctttat tgtccatatc cagacacatt tggtagtatt tagttgcgcc ggcgtaatct
     5581 ttttctgtct cgtaaatatt ggcaaggtaa aatgcagaac ctgccgcata ataataaggt
     5641 aaatcgctgc caaattttat ggtagccaga taaaatcctt ttgctttatc gggcattcct
     5701 gccatgtcgt aaatacgacc ggcgcgatag gtaaattcag ttttatcttt atcgttgcta
     5761 aaactttcgg cgcttttacc ctccagttgt tttaaggcat ctttataata tccgccatca
     5821 aataatagtc gggattttaa cagcgtaaca ttgggcattt ttttgctttc cgattcataa
     5881 agggcttgtt tatcatcatc aattaaatca gcaccgcgag atttaatata atacatatac
     5941 tcccaatact tttctttatt gccatttatc aaagccatcc aggccatttt ttgataggct
     6001 tctttaatgt aatttttacc cttataactc gatataaatt tttcaaaatg tgcacttgaa
     6061 cctgcatcta attttctcac tttggcaaga cctgtcataa attccaggta atggtattgc
     6121 atatattcac ttccggttgg tcgtgatatt aaaacctgca atgccaaatc gtttctgcct
     6181 gttcgcatgg caacactggc tttacaaaat acatttatca aatttgtttt agtgtcaagg
     6241 tccttcacaa tattccaggc agttgttgcg tctctgccca aatacaattc aataaatgca
     6301 taatataaat atacttcctg cttaaaaata aacgtattgg tttttgagta ctcaattatg
     6361 gattttagtt cttttaatcc ctggtcgata gaaccatcca tacccaacat atttacgccc
     6421 catttgtatt ggtctgggat ggcgcccaca atggcgtgta acatgcccaa actttttttg
     6481 ttggccataa aatccggaaa ctttttttca ttttcttcca acaataaata tgctttgcgc
     6541 acatcggtaa aagcagataa atattcttca aatttgagtt ttgaaaacga ccactgaatt
     6601 aacaattcgg cctggcaata taaataataa ggagattgct ggtcgccggt tttaagtttg
     6661 tctaggcgaa tcgttttatt aggttccagc ttatcaaatt ccgcttcatt ttcgttgata
     6721 tatgtcgtta aaaagtcgat ataattttca acgtaatacg gaatgaggtt tttgggattt
     6781 tcttttttct ccagttctaa caattgcctt cctttttcga aacgcaattc ctgaatgtag
     6841 gtataggcat caatacaacg tttattaaaa tcgtattgct gcgcatggga gatgacagga
     6901 aaacaaaatg ccatgaagaa tatgatgata taattcttca tggcatttat caatatgatg
     6961 tgttaaatta acttatgcat ccatagcggc ttcatcaacc aaaatacgac cacagtgttc
     7021 gcaaatgatg atttttttag cctgtttgat ttcagcctga cgctgaggcg gaacaaccgc
     7081 ataacaaccg ccgcaagagt tacgtgaaac ggcaactact gctaaaccgt tgcggtatga
     7141 tgaacgaatg cggtaataag cacccattaa acgctcttcg atttcttttt cagctttatc
     7201 tgcttttttc gcaaaatcag cttcttcttt ttcagtttct tctatgatgc gttccaattc
     7261 ttcttttttc tttttgaggt cttttttctt gttttcaaat acctcttttg catcttcaat
     7321 tgcttttgct ttagcatctt tcagaaaacc cgcttctttc atttttttct cacacaactc
     7381 gatatccaat ttattcaatt cgatttcttt ggtgagggca tcaaattcac ggctgttttt
     7441 aaccgcattt tgctgggttt cgtatttttt aatctgcgtt ttagcgtttg caatcgttgt
     7501 tttgcgaccg gaaatatcgg tttcaatttc cttcaactcc gcttctaact tatgcatacg
     7561 ggtttctaaa ccaacaattt catcttctaa atcgcttacc tccattggaa gttcgccctt
     7621 taagatggta atatcgtcga ttttggaatg tatcttttgg agtttccaaa gcgcctgtaa
     7681 gctttccttt acggacattt ccttcactgc tgccatagtt tataggtagt taataggatt
     7741 agtgttaatt tttgacaaat gaaccgcaaa ggtagcaaat tttttcatca atatgtcgga
     7801 aatcaattca attgtgtatt gttcactctc ataatgccca atatcggcta ttacaatctt
     7861 gttattggca tcgaaaaact ggtggtactt gaagtctgcc gtgataaaca cctctgctcc
     7921 ggccaccata gcgtctttta acaaaaatgc cccggaaccc ccgcaaatag ccactttttg
     7981 gacaggatgt tcgggtaatt tggtatatcg gac
//
LOCUS       DAORKW010000109         8010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_258_length_8010_cov_5.963042, whole genome shotgun sequence.
ACCESSION   DAORKW010000109 DAORKW010000000
VERSION     DAORKW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_258_length_8010_cov_5.963042"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..353
                     /locus_tag="PLB46_06090"
     CDS             42..353
                     /locus_tag="PLB46_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76121.1"
                     /translation="MDINYVPFEEANKEFNAASGAKSIWLQQFLLNRNNPIRKTISFI
                     LRPFRKLVIKSGIIDKVYSMNKKQVEIKPLTAHEIDVAKKYFKDDLTLLEKDYGISFE
                     N"
     gene            425..1486
                     /locus_tag="PLB46_06095"
     CDS             425..1486
                     /locus_tag="PLB46_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRF76122.1"
                     /translation="MRASKYILVMIGSGMLIAFAACGEKKSVDQPVVAGPPPAQPVKV
                     IIVGSENMDNSIDATGTVLANEEVEIRSEIQGRVTGVYFKEGESVAAGKLLISIDDRE
                     LLSQLKRTELSIQLAKDDESRKKQLAAIGGISKEDYDASLNYLLKLEAELELLNTQIA
                     KTKITAPFSGVIGLRYISEGGYVTPANLIATLQQTHPVKIEFSIPEKYSGNIKNGATV
                     NYTVEGNNKLYAATVYAKEPKIDPATRTIKIRATGPNTNNELIPGAFAKLEINLSTIQ
                     DAMILPSEALVPTIKGQSVMLVKNGKATMTVVSTGLRTETTTQIVEGISKGDTVIVTG
                     LLTVRDGMPVKPILSVKSN"
     gene            1498..4566
                     /locus_tag="PLB46_06100"
     CDS             1498..4566
                     /locus_tag="PLB46_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRF76123.1"
                     /translation="MNLSSVSINRPVLATVISILILLFGIIGYSFLGVREYPSVDPPI
                     ITVTTNYIGANADVVESQITEVLEENINGIAGIRSLSSTSADGRSTITVEFELDVDLE
                     AATNDVRDRVSQAVRNLPPDCDPPVVAKTSADSNPIVSMTIQSDQRTLLELSEIANNV
                     FKERLQTIPGVSEIRIWGEKKYSMKLLLDPVKLAGYNLTPLDVRDALNKENVELPTGR
                     IEGYNTELSIRTFGRLTTQEDFNELIIKETEGAVIKLKDIGEAKLLPENERSILRGNG
                     GIPQVAVAITPQPGSNYIDIADEFYNRVDKIKDELPPDLKYEIALDSTISIRKAIDEV
                     KETLIIAFMLVVLIIFAFLRDWRTTLIPVIAIPISLIGAFFIMYIAGFSINILTLLGI
                     VLATGIVVDDAIVVLENIYAKVEKGTPPLQAAHEGSKEIIFAIISTTVTLAAVFLPII
                     FLQGLTGRLFREFGITVAGAVIISAIVSLTLTPMMSSRILKNRERHNWLYRATEPFFE
                     ALIRTYNRSLKAIMGIKRIAYLIVALAAFIIYYFGGKLPEELAPMEDKGRFVINATAP
                     EGTSFEKMDAFNQDLIAFIDTLPEKRNIIAVTAPSFGSSSAANSSFIRVNLVEADLRD
                     KSQQELADEMAALAKKYNFARTFVSQEQTIGGDRRAGLPVQYVIQAPDFEQLRAAIPV
                     FMEKAAQEPAFQVVDINLKFNKPELTVEIDRDRARALGVTVRDIAETLQLYFSGQRFG
                     FFIFNNKQYQVIGQATRDNRDEPLDLSKAYVKNNRGELIQLDNLVTLTDKSSPPQLFR
                     YNRYVSATISANPAPGYTIGDGIEVMDKIAESTLNDDFSTALAGTSKEFAESSNSLQF
                     AFYLALILIYLILAAQFESFRDPLIVMFTVPLALAGAVLTLSIFGQTLNIFSQIGIIV
                     LVGIVTKNGILIVEFANQRKEAGLDKFDAVLEAATQRFRPILMTSFATILGALPIALA
                     LGASSKSRVSMGIAIIGGLLFSLILTLYLIPLLYLMWSSKRKKIAHTNED"
     gene            4556..5869
                     /locus_tag="PLB46_06105"
     CDS             4556..5869
                     /locus_tag="PLB46_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF76124.1"
                     /translation="MKINHLKIAISILFVVTQLTTRAQQLTLNEAKAIMLSNNYGIII
                     QKLETDITGLQNSAGFAGMLPTVTAEAAYNTEISNSEQKYFSGDVRSVENAGANSLDA
                     GVYINWTLFDGFAMFATKDKLNEFENLSQLQLKQQIETAMFNLQAMWFQLIQLKEAET
                     VNKSNIAVSAERLNIASGRERLGAASRLDVLQSEVDLSADSSALLQTQLQLKNVKANI
                     NNLLGRSPETNFDTDEKIEITTGFNYNDLLAKTIEANTDLQIAKNEADIIALSIKEYK
                     AMLYPELNLVGGYGYLRSTSESGFVESNLNYGPSVGLTLSIPLFNGLTTTKNLQIARI
                     EQEKVVTSTQQIQLELNTILLTVYNQYNTSLYLMNLEQKNIAAAQGNVAIAIEKYNLG
                     NITAIELRDIQQSLLQAKNRLLVETLNAKLAELQLLKLSGQLPLN"
     gene            5910..6578
                     /locus_tag="PLB46_06110"
     CDS             5910..6578
                     /locus_tag="PLB46_06110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRF76125.1"
                     /translation="MMIELLLFCTTLFAPQEKTMLCLGDSYTIGQSVQTHERFPELTV
                     ELLAQHNLNFANPDIIARTGFTSQELITNIEKNTPQHTYDIVTLLIGVNDQFRGYDTT
                     TYAKNFETLLKTAIQFANNNAQHVFVLSIPDYGVTPFAANLQSSSEQIADEINAFNSI
                     NQKIANKYNVQYLNITEISRLAKNDNTLLANDGLHPSGKMYGLWAEKLTEQISSSLGK
                     NKTK"
     gene            complement(6587..>8010)
                     /locus_tag="PLB46_06115"
     CDS             complement(6587..>8010)
                     /locus_tag="PLB46_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRF76126.1"
                     /translation="LATVKGDVHDIGKNIVGVVLACNNYAIIDLGVMVPTEKILTTAI
                     AEQVDIIGLSGLITPSLDEMVHVADEMEKRNMTIPLLIGGATTSRTHTAVKIAPAYSQ
                     PVIHVTDASRSVTIAGKLLGAEKNNFIKEIRDEYAIIHTNHKSKQSFKGYISIEEARK
                     NKIILQQKSYIPSIPSFTGVKVFENYPLSELVAYIDWTPFFQTWELHGKYPTIFEDEI
                     IGDAAKKLFDDAQHLLKSIIEHNWLTAKAVVGIFKANSIQDDIIVRNENGETITVLHH
                     LRQQNKKADNQQNYCLSDFILPDNSGITDYIGAFAVSAGFNIEAKVVEFEAQHDDYNS
                     ILLKALADRLAEAFAERMHELVRKEIWGYAKNERTSVDDLIAEKYVGIRPAPGYPACP
                     DHTEKITLFNLLDVTNNIGLQLTESMAMYPAASVSGWYFAHPESKYFGIGKIGKDQVI
                     DLAKRKNMDVAVLEKWLSSNLNY"
BASE COUNT         2670 a         1377 c         1494 g         2469 t
ORIGIN      
        1 aaaacaaccc ggaagcattg tagcgtaact tgggcaatcg attggatatt aattatgtcc
       61 cttttgaaga agccaataag gaatttaatg cagcttcagg ggcgaaaagt atttggttac
      121 agcaattcct tttaaaccga aacaatccaa ttcggaaaac tatatcattt attttgcgcc
      181 catttcggaa gctggtgata aaatctggta tcattgataa agtatattca atgaataaaa
      241 aacaagtgga aattaaacct ttaactgcac atgaaataga tgtggccaaa aagtatttta
      301 aagatgattt aacacttttg gaaaaagatt atggtatttc ctttgagaat taacaatttt
      361 tggtaaactt ttttgatatt attagactta aacaaatatt ctatatctct tatatctata
      421 cattatgcgt gcatcaaaat atattttagt gatgattggt tcaggaatgc ttattgcatt
      481 tgctgcctgt ggggaaaaga aatcagtcga tcaacctgtc gttgcaggtc caccacccgc
      541 tcaacctgtt aaggtaatta ttgtcggaag tgaaaacatg gacaactcaa ttgatgctac
      601 aggtacagtg ttggccaatg aggaagtaga aatacgcagc gaaattcaag gtcgcgttac
      661 aggagtgtat tttaaagaag gtgaaagtgt tgcagcaggg aaattactga taagtattga
      721 tgacagagag ttgctttctc agttaaagag aacagagtta tctattcaat tggctaaaga
      781 tgatgaatcg cgaaaaaaac aattggcagc aattggcgga attagtaaag aagattatga
      841 tgcatcactt aattatctct taaaactaga agcagaatta gaattattaa atactcaaat
      901 agccaaaact aaaattactg caccgttttc gggagttatc ggcttgcgat atatcagcga
      961 aggcggttat gttactcctg ccaatttaat tgcaacatta caacaaacgc atcctgttaa
     1021 aattgaattt agtattcctg aaaaatattc gggtaatatt aaaaatggtg ctacagtgaa
     1081 ttatacagtg gaaggtaata ataaattata tgcagctacg gtttatgcaa aagaaccaaa
     1141 aattgaccct gcaactcgca caataaaaat aagagcaaca ggacctaata ctaataatga
     1201 attgattccc ggtgcatttg caaaattgga aattaatttg tctacaattc aggatgcaat
     1261 gatattgcct tctgaagcat tggttccaac aattaaaggg cagtcggtaa tgctggtaaa
     1321 aaatggtaaa gcaaccatga ctgttgtttc aaccggatta agaacagaaa caaccacaca
     1381 aattgttgag ggtatttcga aaggagatac ggtgattgta acaggattat taactgtgcg
     1441 cgatggaatg ccggttaaac caatcctttc agttaaatca aattaatagt aaaacgcatg
     1501 aatttatcat cagtttctat aaatcgtccc gtacttgcaa cagtaatatc cattttaatt
     1561 ttattatttg gaattattgg ctactccttt ttaggggtga gagaatatcc aagtgtcgat
     1621 cctccaatta ttacggttac cacaaattat attggcgcaa atgctgatgt tgttgaatcg
     1681 caaattacag aggtgcttga ggaaaacatt aatggtattg ctggtatacg ttcgttatca
     1741 tctacaagcg cggatggcag aagtacaatc accgttgaat ttgaattaga tgttgacctc
     1801 gaagcagcaa ccaatgatgt tcgcgaccgt gttagccagg ctgtgcggaa tttaccaccg
     1861 gattgtgatc cgcctgtagt tgccaaaact agtgcagatt ctaatccaat tgtttcgatg
     1921 accattcaaa gtgatcaacg cactttattg gaattatcgg aaattgctaa caatgtattt
     1981 aaggaaagat tacaaacaat tcccggagtt agtgaaatca ggatttgggg ggaaaaaaaa
     2041 tattccatga aattattatt ggatcctgta aaattggccg gatacaattt aacaccattg
     2101 gatgttcgtg atgcacttaa taaagaaaat gtcgaattac caactggaag aatagaagga
     2161 tataataccg aattgtcaat tcgaacattt ggaaggctaa caacacaaga agattttaat
     2221 gaattaatta ttaaagaaac cgagggcgct gtaataaagt taaaagacat tggtgaggct
     2281 aaattattgc cggaaaacga aaggtctatt ctaagaggta atggtggcat tccgcaggtt
     2341 gctgtcgcga ttacaccaca gccggggtct aattatatcg atattgccga tgaattttat
     2401 aatcgggttg ataaaattaa ggatgaatta ccacctgatt taaaatatga aattgcgctt
     2461 gattcaacta ttagtattcg caaagcaatt gatgaagtta aagaaacatt aattatcgct
     2521 tttatgcttg tggtgcttat catttttgcg tttttaagag attggcgcac aacattaatt
     2581 ccggtaattg caattccaat ttccctgata ggtgcattct ttattatgta tatagccggg
     2641 ttttctatca atatattaac gttgttgggt attgttttag caaccggtat tgtggttgat
     2701 gatgccattg ttgtacttga aaatatttat gcgaaagtag aaaaaggcac acctccttta
     2761 caagctgctc acgaaggttc aaaagaaata atatttgcca ttatttctac taccgtaacc
     2821 ttagcagcag tttttttacc tatcattttt ttacagggat taaccggaag attattccgc
     2881 gaatttggaa ttacagtagc gggtgcggtt attatttctg ctattgtttc gttaacactt
     2941 acgccaatga tgagctcccg tattctaaaa aaccgggaac gtcacaattg gttgtatcgt
     3001 gccactgaac cattttttga agctttaatt cgtacatata acaggtcgct aaaagcgata
     3061 atgggtatta aaagaattgc gtatttaata gttgcattgg cagcatttat catttattat
     3121 ttcggcggaa agttacctga ggaattagct ccaatggaag ataaaggacg ttttgtaatt
     3181 aacgcaacgg cacctgaagg aacttcattt gaaaaaatgg atgcgtttaa tcaggattta
     3241 attgcattta ttgatacact gccagaaaaa agaaatatca ttgccgtaac agcaccaagt
     3301 tttgggtctt caagtgctgc aaactccagt tttattcgcg ttaatctggt tgaagctgat
     3361 ttgcgcgata aatcacaaca ggaattggca gatgagatgg ctgcattagc gaaaaagtac
     3421 aactttgcca gaacattcgt aagtcaggag caaacaatcg gcggagatag gagagcaggt
     3481 ttgcctgttc aatatgttat tcaggcgcct gattttgaac aattacgcgc tgctattccg
     3541 gtgtttatgg aaaaggccgc acaagaacct gcatttcagg ttgtcgacat caatttaaaa
     3601 tttaataaac cggaattaac tgttgaaatt gatagagaca gagctcgagc tttaggggtt
     3661 acagttcgcg atatcgcaga aaccttgcaa ttatatttta gcggtcagcg atttggattt
     3721 tttattttta ataataaaca atatcaggta attggtcagg caacccggga taatcgagat
     3781 gaaccactcg atttaagtaa agcctacgtt aaaaataacc gaggtgaatt aattcaactg
     3841 gataatttag taacactaac cgataaaagt tcgcctccac aattatttcg atataacaga
     3901 tatgtttctg ccactatttc tgctaaccct gcgcccggat atacaatagg tgatggtatt
     3961 gaagtaatgg ataaaattgc tgaaagcact ttgaatgatg acttttcaac tgcattagcc
     4021 ggaacttcaa aagagtttgc agaaagcagc aacagtttgc aatttgcctt ttatctggca
     4081 ctaattttaa tttatttaat tctcgcagca caatttgaaa gtttccgcga ccctttaatt
     4141 gtaatgttta cagtcccact tgcacttgcc ggcgctgttt taaccttgtc tatttttggg
     4201 caaacgttaa atatatttag tcagattggt attattgttt tggtcggaat tgttaccaaa
     4261 aatggtattc tgattgttga attcgccaac caaagaaaag aggcgggttt agataaattt
     4321 gatgcagtac ttgaagcagc aacacaacga tttagaccaa ttttgatgac aagttttgcc
     4381 actatcttag gtgctttacc aattgcattg gcgttaggag catcatcaaa aagccgtgtt
     4441 tcaatgggga ttgcgattat aggaggatta ttgttttcat tgattttaac attatactta
     4501 attcctttgt tgtatctgat gtggtcatca aaacgtaaaa aaattgctca tacaaatgaa
     4561 gattaatcat ctcaaaatag ctatatcaat actgtttgtt gttacacaat taacaacaag
     4621 ggcgcagcaa ttaacattaa atgaagctaa agcaataatg ctcagcaata attatgggat
     4681 aataattcaa aaactggaaa ccgatattac cggcttgcaa aattcggcag gctttgccgg
     4741 gatgttacct actgtaacag ctgaagctgc atataatacc gaaatttcaa attcagaaca
     4801 aaaatatttc agcggcgatg tcaggtctgt agaaaatgca ggtgctaatt cacttgatgc
     4861 aggtgtatat atcaactgga ctttatttga tggctttgcc atgtttgcaa ctaaagataa
     4921 attaaatgaa ttcgaaaact taagtcaatt gcaacttaaa caacaaatag aaactgccat
     4981 gtttaatctg caggcaatgt ggtttcagtt aattcaacta aaagaagcag aaacagtgaa
     5041 taagtcgaat atagcagtat ctgcagagcg cctgaatatt gcaagtggca gagaacgttt
     5101 aggtgctgct tcgcgattag atgtgttgca gtcagaggtt gatttgtcag cagacagttc
     5161 agcattattg caaacccaac tacaattaaa aaatgtaaaa gcaaatatca ataatttatt
     5221 aggccgcagc cctgagacaa attttgatac tgacgaaaaa attgaaataa caacggggtt
     5281 taattataat gatttacttg ccaaaacaat cgaagccaat actgatttgc aaatagctaa
     5341 aaatgaggcc gatattatag cccttagcat taaagaatat aaagcaatgt tgtatcccga
     5401 actaaacctt gttggtggat atggatatct gcgttcaaca tctgaatccg gatttgttga
     5461 atcgaatctt aactatggtc cttcggtagg gttaacattg agtataccat tgtttaatgg
     5521 gttgacaact acaaaaaatt tgcaaattgc tcgtattgaa caggaaaagg ttgttacatc
     5581 aacccaacaa atacaattag agctaaatac cattttgttg acggtatata atcaatataa
     5641 tacttcgctt tatttgatga atttggaaca aaaaaatata gctgctgcac aaggtaatgt
     5701 ggcaattgct attgaaaaat ataatcttgg caatataact gccattgagc tacgtgacat
     5761 acagcaaagt ttactccagg ccaaaaacag gttgttggtt gaaacactga atgcaaaatt
     5821 ggcagaattg caattgttga aattaagcgg acaattacca cttaactgat aattgccatc
     5881 caaaccgaca tttaatgcgt atataggtta tgatgataga gttgttatta ttttgcacaa
     5941 cattatttgc acctcaagaa aaaaccatgc tgtgtttggg cgatagctat acgattggcc
     6001 agtctgttca aactcacgag cgatttcctg aattaacagt agaattatta gcacaacata
     6061 acttgaattt cgccaatcct gatataatcg cccgcacggg ttttaccagt caggaactca
     6121 taacgaatat cgaaaaaaat accccgcaac atacttacga catcgttacc ctattaatcg
     6181 gcgtaaacga ccagtttaga ggatatgata ccacaaccta tgctaaaaat ttcgaaacat
     6241 tattaaaaac ggcgatacaa tttgcaaata ataatgccca acatgttttt gtgctgagta
     6301 ttccggatta tggcgtaacg ccattcgcag ctaacttaca aagcagctct gaacagattg
     6361 ctgatgaaat aaatgccttc aattccatta atcaaaaaat agcaaataag tataacgtgc
     6421 aatatttaaa tattactgaa atatcaagat tggctaaaaa tgataatact ttattggcca
     6481 acgatggttt gcatccttca ggaaaaatgt acggactttg ggcagaaaaa ttaactgaac
     6541 aaatcagttc gtcgttggga aaaaataaaa caaaatagta taccgcttaa tagtttaaat
     6601 tactactcag ccatttttct aacacagcaa catccatatt tttgcgtttg gctaaatcaa
     6661 ttacctggtc cttaccgatt ttaccaattc caaaatattt ggattccgga tgcgcaaaat
     6721 accaaccact tacgctagcc gcaggataca tcgccatact ttcagttaat tgtaatccga
     6781 tattattggt aacatccaac aaattaaaca gcgtaatttt ttcagtatgg tcagggcatg
     6841 ccgggtaacc cggagccggt ctgatgccaa catatttttc tgctatcaaa tcatctacag
     6901 aagttctttc atttttagca taaccccaaa tttctttacg aaccaactcg tgcattcttt
     6961 ccgcaaaggc ttctgctaaa cgatcggcaa gtgctttcaa taaaattgaa ttataatcat
     7021 catgttgagc ctcaaattct acaactttgg cttcaatatt aaatcctgca cttacagcaa
     7081 aagcacctat ataatcggta ataccactgt tatcaggtaa aataaaatca ctcaagcaat
     7141 aattttgttg attgtctgct tttttatttt gctggcgtaa atgatgcaat accgtgattg
     7201 tttcaccatt ttcgttgcgc acaataatat catcttgaat agagttggct ttgaaaatac
     7261 caacaactgc ttttgctgtt aaccaattat gttcaattat tgatttgagt aaatgttgtg
     7321 catcatcaaa taattttttg gctgcatcac caataatttc atcctcaaat atagtaggat
     7381 attttccatg taattcccaa gtttggaaaa acggtgtcca gtcaatatac gcaacaagct
     7441 cagataaagg gtaattttca aatactttaa cacctgtaaa agaagggatt gacggtatat
     7501 aacttttttg ctgaagaatt attttatttt ttctcgcctc ttcaattgat atataccctt
     7561 taaacgattg tttcgacttg tgattggtat gtatgatggc atattcatca cgtatttctt
     7621 taataaaatt atttttttct gcacctaata atttcccggc tatggtaaca ctgcgcgatg
     7681 catctgtaac atgaataaca ggctgagaat atgcaggtgc tattttaaca gcagtatgtg
     7741 ttctagaagt agtagcacca ccaatcaata aggggatggt catattgcgt ttttccattt
     7801 catctgcaac atgcaccatt tcatccagcg atggtgtaat taatccgctt aatcctataa
     7861 tgtcaacttg ttctgcaatg gcagttgtta gaattttttc agtcggcacc attacgccta
     7921 aatcaataat agcataatta ttacatgcaa gcacaacgcc aacaatattt tttccaatat
     7981 catgtacatc gccttttaca gttgcgagta
//
LOCUS       DAORKW010000110         8003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_259_length_8003_cov_4.709361, whole genome shotgun sequence.
ACCESSION   DAORKW010000110 DAORKW010000000
VERSION     DAORKW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_259_length_8003_cov_4.709361"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLB46_06120"
     CDS             complement(<1..410)
                     /locus_tag="PLB46_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76127.1"
                     /translation="MHIPLEDFFRNPKQTGFHIAPDGQHISWLETYADRLNIFIQNLT
                     SKETRQLTFQTDRDISNYFWLNNEQIGVLKDNNGDENHHLYAINCANGAMTDLTPFEN
                     VKVDVVDELDDNDDEILIMMNQRNPVLFDVYRLNT"
     gene            595..1254
                     /gene="rpe"
                     /locus_tag="PLB46_06125"
     CDS             595..1254
                     /gene="rpe"
                     /locus_tag="PLB46_06125"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRF76128.1"
                     /translation="MSHLVAPSILSCDFAYIARDVEMINNSAADWIHVDVMDGVYVPN
                     MSFGIPVVAAMKRHSRKPLDVHLMTVTPERYLKQFREAGADSISVHVEACTHLHATLQ
                     EIKKLGAKAGVAINPGTSISVLEDIITDIDIVCFMAVNPGWGGQSFIETSIEKIKKLR
                     ALINQTGSKALIEVDGGVKMDNAQRILDAGVDVLVSGSGVFGEPDPIAAIQTLKSLVR
                     K"
     gene            1256..3739
                     /locus_tag="PLB46_06130"
     CDS             1256..3739
                     /locus_tag="PLB46_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF76129.1"
                     /translation="MQKTAFVEVMFRSFLTTLFLLVSLSSFAQQTATVYGKITDTDGK
                     AIPYANVIAIDRSIIAQSDVDGNYSIKIPANDTVVLKYSNLSDTAYRKVFLAPDERYV
                     IIVKLSGYQLGTVEINVKETIFRIPVEKLEPIPYTMDPLTAILQAMGAVINNELTSQY
                     SIRGGNFDENLVYVNDFEIYRPLLVRSGQQEGLPFPNYDLIDNIAFSAGGFEAKYGDK
                     LSSVLDIQYKKPTEFEAGVTASLLGASFYFNDKPDSSKIYYLFGTRYKTNKYLLNSLN
                     TAGQYEPIFVDAQGLLGIQLNKKSSLEFLGNFSYNSYTFIPESRSTATGLVNEVIQLD
                     VFFDGQEIDQFITGFGGVSYNYQPTKSTVYKFLASGYHSLESETFDIIGEYWLGLVET
                     NLGDEDFGDVIYGLGVGTFQDFARNYLTSDVINIGHVGSHSNLVETHFLQWGVRAQYE
                     LINDELKEWELLDSAGFSLPYTGEEVQLQEVFKSNIALNSMRYNAYIQDAWEPQQKHF
                     ALTYGIRASYWDLNKEFTITPRVQYIWQPEWYRKDTTVNIEFKAAAGLYYQPPFYREL
                     RNLEGEINENVKSQKSVHLLIGSDYKFQAWDRDFRFITELYYKYMFDLIPYDLENVRI
                     RYYGENQAVGYAAGIDLRLHGEIVKDAESWVSLSVMRTKEDIENDSTIILNKDEDGNI
                     TSYTIVEQGYIRRPTDQLINFGMFFQDYMPGNENFKVHLSFLFGSGLPYGPPNNEYYR
                     NASKIPPYRRVDIGFSASLLDKDRNLENKKPINKAFESMWASIEVFNLLGIQNTISYI
                     WVKDVNNQQYSFPNYLTDRRLNFKIVAKF"
     gene            3766..4158
                     /locus_tag="PLB46_06135"
     CDS             3766..4158
                     /locus_tag="PLB46_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRF76130.1"
                     /translation="MKVFLSLIFICCFFRLPAQVIDISQPPQEPFENIKAIQVYSDSL
                     ATTFIIYIKHEVALHKHEWHTEQVYILEGTATMRLGDKWFDIKANDFIVIPQNTLHAV
                     KVTSSTPLKVMSIQSPYFDGTDRVMITE"
     gene            complement(4166..4627)
                     /locus_tag="PLB46_06140"
     CDS             complement(4166..4627)
                     /locus_tag="PLB46_06140"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRF76131.1"
                     /translation="MWKFFLFLIINFLALGIGSWLMGNPATNEWYNGLNKAPWTPPGW
                     VFGAAWFTVMFCFAVYMFKGASLFPPEKLNLLYVLFAIQFVLNVLWNPVFFRWHFVAP
                     GLIIISLLAIVMVIMLVFGKNQMGWNTLWVLPYVLWLCIATSLNAYVLFKN"
     gene            complement(4680..5615)
                     /locus_tag="PLB46_06145"
     CDS             complement(4680..5615)
                     /locus_tag="PLB46_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF76132.1"
                     /translation="MDLKQTTNILLIEDNPADARLVEIYLNESSMIIPKISRVAELKK
                     GLFMLEESDFDVVLLDLTLPDSSGFDTVKTILNEFPGQTVIVMTGLEDDTVAINSVKA
                     GAQDFIVKGQFDSNLLSRTITYAIERHQLQIKLEDYAKAIKLNEQRLIEAQTMARMGN
                     WELDVVNNQMYWSNEVFRILGFKENAIEPTLNDYLKYVAPEDINNVRNAIKRTMEKGQ
                     PYHIEYAVVLPGNVRKTIANQGLIQKSKKSNGLCLAGTIQDISNLSALHDGHSINIEA
                     LNQQLQLIKTEAAKLKGAEHLQKLINDAADLLQTK"
     gene            complement(5623..6072)
                     /locus_tag="PLB46_06150"
     CDS             complement(5623..6072)
                     /locus_tag="PLB46_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF76133.1"
                     /translation="MKSQKDDVIQILLVEDNPGDIRLTQEALKEGTIRNELHVVKDGV
                     EAIEFLKRKGKYIAHPTPDIILLDLNLPRKDGREVLAEIKSDENLKLIPVIILTTSDA
                     DLDIQKSYKLHANCFITKPVDLDQFIFIIRQIETFWFTVVKLPAKKY"
     gene            complement(6168..6845)
                     /locus_tag="PLB46_06155"
     CDS             complement(6168..6845)
                     /locus_tag="PLB46_06155"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRF76134.1"
                     /translation="MKRATFILGIVSLITTLMAFSPMPPTNSIALLKYNGGGDWYANP
                     TSLPNLAEFCNKQLDININTDPPTVEVGSPDIFNYPFIHMTGHGNVVFSAEEAQNLRT
                     YLIAGGFLHVDDNFGMDPYIRPALKKVFPELDLVELPFSHPIFHQRYDFKNGLPKIHE
                     HDNKPPRAFGLFWEGRLVCLYTYECDLGDGWEDADVHKDPEEVRMKALQMGANIIQFV
                     FMDNTSK"
     gene            complement(6842..7555)
                     /locus_tag="PLB46_06160"
     CDS             complement(6842..7555)
                     /locus_tag="PLB46_06160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRF76135.1"
                     /translation="MHWLYAPDLHDQTQFTLSESESHHLINVLRAKSGDQIIITNGNG
                     RLFEAVIAVAHKKHTTVNIIATSNFTRTQGSIHLVIAPVKTAERLGFLLEKITELNVT
                     SITPILTQNGERRVFNHEKEIHHLIAAIKQSKNPFLPILHPLTTFEKLLTDFQRNVAQ
                     KLICHCRNTPKMKLTQQYQPKQDVIICIGPEGDFTTEEVAAAEQAGFTSVSLGNEVLR
                     AETAGIVAAVTIKTINELS"
     gene            complement(7560..>8003)
                     /locus_tag="PLB46_06165"
     CDS             complement(7560..>8003)
                     /locus_tag="PLB46_06165"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF76136.1"
                     /translation="LFFNGGLGIAWESETGYFGIAVPAILTPRRNNSNDTIALNAPQL
                     NLNGAYTFILSSNWKLTSSLYYGYINTLPSQMALMFDFTFKNNYAFLAGIRSNNAYTA
                     GFNYTFLDNFKIGYTYDYFDNNLGAAAGGGHELVIGFDLNKPEGE"
BASE COUNT         2490 a         1419 c         1518 g         2576 t
ORIGIN      
        1 gtatttaatc ggtaaacatc gaacaaaacc ggatttcgct gattcatcat aatgaggatt
       61 tcatcatcat tatcgtccaa ctcatctacc acatcaactt ttacattttc aaatggtgtg
      121 aggtctgtca ttgctccatt cgcacaattg atagcatata aatgatgatt ttcatcgccg
      181 ttattatctt ttaatacacc tatttgttca ttattgagcc aaaaataatt gctgatatct
      241 ctgtcggttt gaaaggtgag ttgtcgggtt tcctttgaag ttaaattttg tataaaaatg
      301 ttcaacctat ctgcataagt ctccagccac gaaatatgct gtccatcggg agcaatgtga
      361 aatcccgttt gttttgggtt acgaaaaaaa tcctctaatg gaatatgcat ctatataatt
      421 ttgtgtaaag ttacctactt gaggttttgt ttacaggaca aagttctgct attcatagtt
      481 cagtaagcta cttttttcga caagtagagc cattttttcg acacgctact caatttgaga
      541 gatggagttt tcgatcggtt tatttcttac aatataagca cttatctttg cggcatgagc
      601 catttagttg cacccagtat attaagttgt gattttgctt atatcgccag agatgttgaa
      661 atgataaaca attcagccgc agattggatt cacgtggatg ttatggacgg cgtgtatgtg
      721 cccaatatga gttttggtat accggtggtg gcagctatga aacgccatag ccgcaagcct
      781 ttggatgtgc atttgatgac ggttacccct gagcgttacc tgaaacaatt tagagaagcc
      841 ggtgccgatt caatttcagt tcatgtggaa gcttgcaccc atctgcatgc cacattgcag
      901 gaaataaaaa agttaggcgc aaaggcaggt gtggcgatta atccgggaac ttctatatcc
      961 gttttagaag acatcattac agatatcgat attgtttgtt ttatggccgt gaacccgggg
     1021 tgggggggac aaagttttat agaaacttcg attgaaaaaa taaaaaaact gcgtgcactc
     1081 atcaaccaaa caggctcaaa agccttgata gaggtggatg gtggcgtaaa aatggacaat
     1141 gcacagcgca ttttagatgc aggtgtcgat gtgctggtat ccggaagcgg tgtatttggc
     1201 gaaccggacc ctattgctgc aatccaaaca cttaaatcgc tcgtaagaaa ataatatgca
     1261 aaaaacggcg tttgttgaag tgatgtttcg cagcttttta accacactat ttcttctagt
     1321 gtctttgtcc tcatttgctc aacaaacagc aactgtatat ggaaaaatta ccgatactga
     1381 tggcaaggca attccttatg ccaatgtaat tgcaattgat cgcagcatta ttgcgcaaag
     1441 cgatgtagat ggtaattatt ccatcaaaat tccggcaaat gatacagtag ttttaaaata
     1501 ttccaattta agtgatactg cttatcgaaa agtattttta gcacctgatg aacggtatgt
     1561 aattattgta aaattatcag gttatcaatt gggtactgtt gaaatcaatg taaaagaaac
     1621 catatttcgt atccctgtcg aaaaacttga acctatacca tatactatgg acccgttaac
     1681 agccatattg caggcaatgg gagcggtaat taataatgag cttacttcac aatattctat
     1741 tcgaggtggc aacttcgatg agaacctggt atatgtcaac gattttgaaa tttatcgacc
     1801 attgttggtg cgttcaggac aacaggaagg tttgccattt cccaattacg atttaattga
     1861 taatatcgcc ttcagtgccg gcggatttga agctaaatat ggggataaac tctcgagtgt
     1921 attagatatt caatacaaaa agcctacaga atttgaagca ggtgtcacag ccagtttgtt
     1981 aggtgcttcg ttttatttta atgataaacc cgattccagt aaaatttatt atttattcgg
     2041 tacccgatat aaaaccaata aatatttact caactcacta aatactgcag gccaatacga
     2101 accaatattt gttgatgcac aaggtttact cggcattcaa ctcaataaaa aatcatcatt
     2161 agaattttta ggcaatttca gttataacag ttatacattt attcctgaaa gcagatcaac
     2221 agcaaccggt ttggtgaatg aagttattca gttagatgta tttttcgacg ggcaggaaat
     2281 tgatcagttt attacaggtt tcggcggggt ttcttataat tatcaaccaa caaaaagtac
     2341 cgtatacaaa tttttagcat cgggttacca cagtttagaa tctgaaacgt ttgatattat
     2401 tggagaatat tggttaggct tagttgaaac caatttaggt gatgaagatt ttggagatgt
     2461 aatttatggt ctaggtgttg gtacttttca ggattttgcc agaaattatc tcacatctga
     2521 tgttattaat attggtcatg taggttcaca tagtaatctt gtagaaacac attttttaca
     2581 gtggggggta cgcgctcaat atgaattgat aaatgacgaa ttaaaagaat gggaattact
     2641 ggacagcgca ggtttttcgt tgccttacac cggagaagaa gtgcaattgc aagaagtatt
     2701 taaaagtaat attgcgctca acagcatgcg ttacaatgcg tatattcagg atgcatggga
     2761 accacaacaa aaacattttg cgttaactta tggtatcaga gccagttatt gggatttgaa
     2821 taaagagttt accatcacac ctcgtgtgca atatatttgg caacccgaat ggtatagaaa
     2881 ggatacaaca gtgaatattg aatttaaagc ggcagcaggt ttatattatc agcctccgtt
     2941 ttatcgtgaa ttgcgcaacc tggaaggaga aattaatgaa aatgtaaaga gtcagaaaag
     3001 tgtgcacctt ttaattggaa gtgattataa atttcaggca tgggatcgcg acttcagatt
     3061 tataactgaa ttgtattata aatacatgtt cgaccttatt ccttatgatt tagaaaatgt
     3121 gcgtatacga tattatggtg aaaaccaagc tgttggttat gctgcaggta ttgatttacg
     3181 tttgcatggt gaaattgtga aagatgcgga aagctgggtg agtttaagtg ttatgcggac
     3241 aaaagaagat atcgaaaatg atagtacgat aattttgaat aaagatgaag atggcaatat
     3301 tacttcttat acgatagttg agcaaggtta tattcgcaga ccaactgacc agctgatcaa
     3361 ctttggtatg tttttccagg attatatgcc gggtaatgaa aattttaaag tgcatttaag
     3421 ctttttattt ggcagtggtt taccatatgg ccctccgaat aatgagtatt atcgcaatgc
     3481 ttctaaaatt cctccttacc gccgcgtgga tattggtttt tccgcttctt tgttagacaa
     3541 ggatagaaat cttgaaaata aaaaacccat taataaagcc tttgaaagta tgtgggccag
     3601 tatagaggta tttaacctgc tgggcataca aaataccatc agctatattt gggtgaagga
     3661 tgtaaataac cagcaatatt ctttcccgaa ttatttgaca gaccgcagat taaattttaa
     3721 aatcgttgca aaattttaat acttttaggt attcaaattg atgcgatgaa agtatttctc
     3781 agtttaatat ttatatgctg cttttttaga ctacctgctc aggtaattga tatatctcaa
     3841 ccacctcaag agccgtttga aaatataaag gctattcagg tgtattctga ttcactagct
     3901 actacattta ttatttacat taaacatgaa gtggcattac ataaacatga atggcatact
     3961 gagcaagtat atattctgga aggaactgca accatgcgtt taggtgataa atggttcgac
     4021 attaaagcaa atgactttat tgtaattccg caaaacaccc tccatgcggt aaaagtaact
     4081 tcttcaaccc ctttaaaagt aatgagtatt caaagtccgt attttgatgg tactgaccga
     4141 gtgatgatta ctgaataaat gtaatttaat tcttaaacaa cacgtaagca tttagagaag
     4201 ttgctataca cagccataat acataaggta atacccaaag tgtattccat cccatttgat
     4261 tttttccgaa caccaacata attaccatta caattgcgag taaggatatt atgatgagtc
     4321 ccggcgctac aaaatgccat ctgaaaaaaa caggattcca caatacattt agcacaaact
     4381 gaatggcaaa aagtacatac agcaagttca gtttttcggg aggaaataaa ctagctcctt
     4441 tgaacatata taccgcaaaa cagaacatta ccgtgaacca tgcagcacca aaaacccaac
     4501 ccggtggtgt ccaaggagct ttgttaagac cattatacca ctcgtttgtg gcaggattgc
     4561 ccattaacca gctaccgatg cccagcgcaa gaaagttgat aatcaggaat aaaaagaatt
     4621 tccacatatc tatttaacaa aaataaatac gataatgttc attaagccat tagttgtggc
     4681 tatttagttt ggagtaaatc agcagcatcg ttgataagct tttgcaaatg ttccgcacct
     4741 tttaattttg ctgcttctgt tttaattaat tgtaattgtt gatttaatgc ttcaatattg
     4801 atggagtggc catcgtgaag tgcagataaa ttagaaatat cctgtatagt acccgctaaa
     4861 caaaggccat tacttttttt acttttttga attaaacctt ggttagcaat tgttttgcgg
     4921 acattacccg gcaatactac tgcatattca atatgataag gctgtccttt ttccatggtg
     4981 cgtttaatcg cattgcgcac attattaata tcttcgggag caacatattt taagtaatcg
     5041 ttgagggtag gttctatggc attttcttta aaaccaagga tacgaaatac ctcattactc
     5101 caatacattt gattatttac cacatcgagt tcccagttac ccatacgcgc cattgtttgt
     5161 gcttcaatta accgttgttc gtttaatttt atcgccttgg cataatcttc caatttaatt
     5221 tggagttggt gacgttcgat ggcatatgtg atagtacgtg ataataaatt gctatcaaat
     5281 tgtcctttta caataaaatc ctgagcaccg gcttttactg aattaatagc taccgtatca
     5341 tcctccaaac cggtcattac aattacggtt tgacccggga attcatttaa tatggtttta
     5401 acggtatcaa accctgagct gtcaggaagt gtaaggtcaa gtaatacgac atcaaaatca
     5461 ctttcttcta gcatgaataa accctttttt aattccgcta cgcgcgaaat tttaggtata
     5521 atcatggatg actcattgag ataaatttcc accaatcttg catcggcagg attatcttca
     5581 attagtagga tgttggttgt ttgtttaagg tccatcaggg ttttagtatt tttttgccgg
     5641 tagttttaca actgtaaacc aaaatgtttc tatttgtctg atgataaata taaactgatc
     5701 taaatctact ggtttggtga taaaacaatt ggcatgtaat ttataacttt tctgaatgtc
     5761 taaatcagca tcagaagtgg tgagtatgat tacgggtatt aactttaagt tttcgtctga
     5821 ttttatttct gccaatactt cacgtccatc tttacgaggc aggtttaaat ctaataaaat
     5881 aatatcgggt gttggatgtg caatatattt gcccttgcgc ttcaaaaatt cgattgcttc
     5941 cacgccatct ttaaccacat gtagttcatt tctaattgtc ccttctttta aagcttcctg
     6001 ggtcaatcga atatcaccgg ggttgtcttc caccagtaaa atttgtatta catcatcctt
     6061 ttgggatttc atcttgacag ttaatcaatt agataatgaa attactcccc cccccaacaa
     6121 cgaaagtatg aaaaaaatat taaattatcc aagcaatagt aaggtattca cttagatgtg
     6181 ttatccataa atacaaactg aatgatatta gcccccattt gtagggcttt cattctaacc
     6241 tcttcagggt ccttgtgcac atcggcatct tcccagccat cacctaaatc gcactcataa
     6301 gtatataaac atactaaacg accttcccaa aacaagccaa atgcccgtgg tggtttatta
     6361 tcatgctcat gaattttggg taagccattt ttaaaatcgt aacgctgatg aaatatagga
     6421 tgcgaaaaag gtaattctac taggtccaat tcaggaaata cctttttcaa tgcaggccga
     6481 atataagggt ccataccaaa gttatcatca acatgcaaaa aaccgcctgc aattaaatag
     6541 gtgcgcaggt tttgagcttc ttcagccgaa aacaccacat tgccatgtcc tgtcatatgg
     6601 ataaacggat aattaaaaat atcaggactt ccaacctcaa ctgtcggagg gtccgtattg
     6661 atattaatat cgagttgctt attacaaaat tcagccagat tcggtaatga ggtagggttc
     6721 gcataccagt cgccaccacc attatatttt aataatgcaa tagaattagt tgggggcatt
     6781 ggtgaaaatg ccatgagggt ggtaattaaa ctaactattc caagtataaa tgtggctcgt
     6841 ttcatgacaa ttcgttaatg gttttaatgg ttacggcagc tacaataccg gctgtttctg
     6901 cccgaagtac ttcattgccc aagcttacgg aggtaaatcc ggcctgctct gctgctgcaa
     6961 cttcttcagt ggtaaagtca ccctcagggc ctatacaaat aattacatct tgcttaggtt
     7021 gatattgttg tgtcagtttc atttttggtg tattgcgaca atgacatatc aatttttgtg
     7081 ctacatttcg ctgaaagtca gttaataatt tttcaaatgt tgttaaggga tgtaatatcg
     7141 gaagaaacgg attttttgat tgtttaattg ctgcaatgag gtgatggatt tctttttcgt
     7201 gattaaaaac cctgcgttcc ccattttgag ttaaaattgg ggtgatggag gttacattta
     7261 attcagtaat tttttctaac aaaaacccta aacgttctgc cgttttaacc ggtgcaataa
     7321 caaggtgaat actgccttgt gtgcgtgtaa aattgctggt agcaataata ttaacagtgg
     7381 tgtgtttttt atgcgcaact gctatcactg cttcaaataa acggccatta ccattggtaa
     7441 taataatttg atcgcccgat tttgctctta atacatttat gagatgatgc gattcacttt
     7501 cgcttaaagt gaattgcgtt tggtcatgta aatcaggagc gtataaccaa tgcatacttt
     7561 tattctcctt caggtttatt taaatcgaag ccaattacca actcatgtcc gccaccggct
     7621 gcagcgccaa gattattgtc aaaataatcg taagtgtaac caattttaaa attatctaaa
     7681 aacgtataat taaatcctgc ggtataggca ttattgctgc gaatgccggc taaaaatgca
     7741 taattatttt tgaaggtaaa atcaaacatc agcgccattt gtgaaggcaa tgtattgatg
     7801 taaccataat acaacgaaga ggttaatttc caattggatg ataaaataaa tgtataagca
     7861 ccgttcaaat ttaattgagg tgcatttagt gcaatagtgt cgtttgaatt atttcttctt
     7921 ggggttaata ttgccggaac cgcaatacca aaataaccgg tctccgattc ccatgctatg
     7981 cctaatccgc cattaaaaaa tag
//
LOCUS       DAORKW010000111         7994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_260_length_7994_cov_5.575135, whole genome shotgun sequence.
ACCESSION   DAORKW010000111 DAORKW010000000
VERSION     DAORKW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7994
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_260_length_7994_cov_5.575135"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PLB46_06170"
     CDS             complement(<1..474)
                     /locus_tag="PLB46_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76137.1"
                     /translation="MKKIFGLLVISLWLSGCKQSDPKDVLGREGDVVYFSGYVWDVKI
                     NEFAVGPGPNYFSGFYEDIYIDENGYLHMRIAEHNGKWYSTEIVGRDTLGYGKYTWVM
                     QADLENIPENITVGLFTWDNESFFEEANSEVDIEFSKWGNPDLATTLHYSVQPVAF"
     gene            complement(480..2726)
                     /locus_tag="PLB46_06175"
     CDS             complement(480..2726)
                     /locus_tag="PLB46_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRF76138.1"
                     /translation="MKQLQNNVVRKIKNSIGIFFITSTLHAQSNVDIDALIAKMTIEE
                     KVGQMTQLNLDVVCEGGIYNLVEPHHIEPAKLHKALVEYHVGSILNCGGHAYGLDQWH
                     TIINTIQQVATTETRLKIPVIYGIDAIHGANYTLGSTLFPQPLAQAASWNTDMVKRGG
                     EITAYEVRATGIPWNFSPVLDLGRDPRWSRLFETYGEDVLLATEMGLATIEGYQGNNL
                     ADAYHVAACMKHFLGYSMPRTGKDRTPVYIDEIQLREYFLPTFQAAINAGAATVMINS
                     GELNGIPVHADKKILTDLLRSELGFKGVAVTDWEDIMKLNNIHMVAPTLKDAVYMAID
                     AGIDMCMVPNDYDFTILLTELVKEGKISEARLDVSVKRILELKKQLGLFEQPFTPANT
                     DYKKFGSAEFARAAQNMVEESITLLKNNNQTLPIKTETKIFVTGPAANSMTYLNGAWT
                     RTWQGTDTIWNDQSKYTIREAFNAATTTFIASDNMSDASVREKMLAAAKASDVIIICL
                     GEKPSTEKVGDIDALTMPDNQQQLVGLLKSVGKPIVLVLVENRPLIVNDIEPLCDAIV
                     LAYEPGDYGGLALANIIYGKVNPSGKLPFTYPRHEASLLWYDHKHTETLDQTFGRKAF
                     NPQWEFGYGLSYTTFQYSNLKISNDTLSTGNLQISVDVTNTGNSAGKEVVQLYIADRY
                     ASITPSVKRLRGFEKVEIKPGATHIVTFTISAKDLAFVGRDEQWMSEAGWFDIMLGSE
                     KSSFYLSK"
     gene            complement(2707..3948)
                     /locus_tag="PLB46_06180"
     CDS             complement(2707..3948)
                     /locus_tag="PLB46_06180"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRF76139.1"
                     /translation="MTTSSGNIIKLLLAGLIAPTLFSCATKSVSIYDTAKSDSIPHIK
                     NVYAVNIFSDYVTSELWKTENNACLQLQNKFDSSMIGAGGLYLKWNKTSGGCDWVGMG
                     IGWDGWNGKNLSEIYNEAAIQFMVRSPKGTQPGLPWAMAFEDFGGGQAWAGVFSNFIE
                     GGKITEQWTKVQIPLTAFDFNQFDADKSGIKQLLIQFESEGEIFLDEMKIVPTQTIAS
                     KSVDVPYVNSLPTMDGVKESFFIGDGFQLENGTVWVAVNDKNVMLFAEVQDASPLQNN
                     QNGKDIWNGDALEIAFSTNSNAGTNRKAFLLSDQHFGIRATSTPVLWNWRSGTNTKGT
                     VITKTTKTGYVIEAVIPMEQFIAAPLVAGNNYQIEIAIDAGTTKGRDKQYRWNNPYNE
                     GFHTSPQLWGQMRIINETATK"
     gene            complement(3948..4952)
                     /locus_tag="PLB46_06185"
     CDS             complement(3948..4952)
                     /locus_tag="PLB46_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76140.1"
                     /translation="MKKIFLPLFSAVLFLASCSKDDSGFDGPSLNDIYGDFSILDSLS
                     ASAATVDFSAGQQLTFSAEFSKNVNWKIAITGNTSGAVYTIEGFSRLIDATNAIWDGS
                     ATTLPMFRSEDCVAQLTIDGEDDTLTAPISVLGTKVITGLILSDFEGEFNPGWSTFVQ
                     SGADMSFIITDSDPAAQGSKYYDMGGTVDWDWLLGLIDIPGSAYGATTFALNSNPNNV
                     YFNVMLSKIPGLNNGLVLFQFREDDNGDGVYTDGEEDLFSIEVALNNEDGWQLISSKY
                     ADMPTLVNGAPVDPIGNGIYEPEKLIQVSILMLANPVSGYAQAYMDLMLFTENQPFKP
                     "
     gene            complement(4958..6688)
                     /locus_tag="PLB46_06190"
     CDS             complement(4958..6688)
                     /locus_tag="PLB46_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76141.1"
                     /translation="MKKITSTIFTGLILCSALTIQAQTEVKKFSLNGTARTLSFLDNL
                     SQDVEVADTITAPKSLSSHTLVDLGMLIRPGDNIAIQSSVRIRNDFGGFWGSGVTFDV
                     RELYVKGVVKDVVRYQVGDVHYKLTPYTFYNNDSELSNYTPGAFSHLLDQVSYDNFYL
                     GDNTWSQQGAAVDFGVVFNKYVQEINWNVFTGRVAATNGGNQPERLFSAANMTLIQSK
                     YVSLGVNYANFYDFPGTASTTEQYVNPVVTGNAQVAYTFGDIALTANGEMGKSHNEIK
                     NDSLAPVLDGNFFDTKIEAKYTPWQLGIYIGMKSVGADFRSAGAQTKRINFDGTPAAY
                     ERITNDQILRETTMFDLLREADLYNLQLQNGLMAFDARYDNITPYGAASPNRQGFEVG
                     LNWVGCKEAVKLNASFLSQSEIRGQGTLNLRNFARMDANATIAINKLVKWKKSIELTA
                     GMRSDNTSRDGEAGVPSVELQTDVLSVGFSANIVSDLDIVLGLQQHTYAGFEFVNQTD
                     KYDQIINFTEYTVDGSQQMIGGGLRWNFSEKTYLMGQFNQFSQNNNLVDDVLPSYTIN
                     NFAFIYSMQF"
     gene            complement(6688..>7994)
                     /locus_tag="PLB46_06195"
     CDS             complement(6688..>7994)
                     /locus_tag="PLB46_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76142.1"
                     /translation="KYYNEGAVDQDVRWGNRAFKGFVLEGAELPGDLKAKILIGKTEL
                     NGGFSSTPNMSYGGQLVKQLNNGRYYGINTISSITYADSLALEKVGFNTLTFEVNEKI
                     KSFGLHVEAGMAQYFSPLHNEGWGQMLSGTITSPIKKNIPVISLHGFSIAPEVFNNNA
                     VFLNSSVTEYTTNDIPAGSIGSSAVLRPTGSAMLRMGMMSNNRKGLDLNIEQTWDNIS
                     FNIGFGMNGEITPMSNVITYSHPINSLTRARFWRFAFPSNVGPYDRYSVIYRDVYETV
                     NLTDDSSGVAVNKKYFSVFEPQIKFKTVAFNRPLYIFYLGYYSTAQRDWSVIPVLNEE
                     AYIRQYASEFEIYYSITDGLVLSGYYGYERTIGNYLTDIDEVTRRPRNQTGNGYGAGL
                     DIDLGRNAILYIRNRWANFSDASFADDTFRNQETIVELKIFF"
BASE COUNT         2405 a         1693 c         1304 g         2592 t
ORIGIN      
        1 aaatgcaaca ggttgcaccg aataatgtaa ggtggttgct aaatcgggat taccccattt
       61 tgaaaattct atatctactt cgctgttcgc ttcttcaaaa aatgattcat tatcccaggt
      121 aaataatcca actgtaatat tctccggaat attttctaag tcggcttgca tcacccaggt
      181 gtatttacca taccctaatg tatcgcgacc aacaatttcg gtgctatacc atttgccgtt
      241 gtgttcggca atgcgcatgt gtaaataacc attttcatca atataaatat cttcataaaa
      301 acctgaaaaa taattcggtc cgggaccaac tgcaaattcg ttgattttaa catcccatac
      361 gtatccggaa aaatacacca catcaccttc gcgacctaat acatctttcg ggtctgattg
      421 tttacatcca ctcaaccaca aactaattac caataatccg aatatttttt tcatactgtt
      481 tattttgata aataaaacga agatttttca ctgcctaaca taatatcaaa ccaaccggct
      541 tcactcatcc attgttcatc gcgaccaaca aatgcaaggt ctttggcact tattgtaaaa
      601 gttacaatat gtgttgcgcc cggcttaatt tctacttttt cgaagccgcg taatcttttt
      661 acagaaggtg taatactcgc atacctgtct gcaatatata attgcacaac ttcttttcct
      721 gctgagtttc ctgtgtttgt tacatcaaca ctaatttgca aattgcctgt gctcagcgta
      781 tcgtttgaaa tttttaaatt actgtattgg aaagtagtat aacttaaccc ataaccaaat
      841 tcccattgcg gattaaatgc ttttctgcca aatgtttgat ctaaggtttc agtatgttta
      901 tggtcgtacc ataacaacga tgcttcatgg cgcggataag taaatggcaa ttttcccgat
      961 ggatttactt ttccataaat aatattagcc aatgctaatc caccgtaatc gccgggttcg
     1021 taggcgagca caatggcatc acataatggt tcaatatcat ttacaattaa agggcgattt
     1081 tctactaaaa ctaaaacaat gggtttgcct acagatttta ataaaccaac taattgttgc
     1141 tgattatcag gcatggttaa cgcatcaata tcacccactt tttctgtcga tggtttttcg
     1201 ccgagacaaa taataattac atcacttgct tttgcggcag ctaacatttt ttcgcgcacg
     1261 ctggcatcac tcatattatc tgatgcaata aatgttgttg ttgcagcatt aaatgcttcg
     1321 cgaattgtgt atttgctttg gtcgttccag atagtatccg ttccctgcca ggttcttgtc
     1381 catgcaccat ttaaataggt catactgtta gccgcaggtc ccgtaacaaa tatttttgtt
     1441 tctgttttta tcggtaaggt ctgattatta ttttttaata acgtaatgga ttcttccacc
     1501 atattttgtg cagcacgggc aaattcagca gagccgaatt ttttataatc ggtattggca
     1561 ggcgtaaatg gttgttcgaa taagcccaat tgttttttta attccagaat tcgttttacg
     1621 gaaacatcta aacgcgcttc tgatattttt ccttctttca ctaattcggt taataaaatg
     1681 gtaaaatcat aatcattggg aaccatacac atatcaatac ccgcatcaat agccatatac
     1741 acagcatctt ttaatgttgg tgcaaccatg tgaatattat tcaacttcat gatatcttcc
     1801 caatcggtaa ccgctacacc tttaaatcct aattcactgc gtaataaatc ggtaagaatt
     1861 tttttatcgg cgtgaacagg aataccattt aattcaccac tgtttatcat tacagttgca
     1921 gctccggcat ttattgccgc ttgaaaagtt ggcaaaaaat attcgcgtaa ttgaatctca
     1981 tctatataaa ccggcgttct gtctttacct gttcgtggca tgctataacc taaaaaatgt
     2041 ttcatgcatg cagcaacatg ataagcatca gctaaattat ttccctgata accttcaatt
     2101 gttgctaatc ccatttcggt agctaataaa acatcttcac cataggtttc aaataaacgc
     2161 gaccaacgcg ggtcacgacc taaatctaaa accggtgaaa aattccaggg aatacctgtt
     2221 gcgcgcactt cataagctgt gatttcacca ccgcgtttca ccatatccgt attccagctg
     2281 gcagcttgag ctaaaggttg cggaaataat gttgaaccta atgtataatt agcaccgtga
     2341 atggcatcta taccataaat caccggaatt tttaatctgg tttcagtagt tgcaacttgt
     2401 tgaattgtat tgatgatagt atgccattga tccaatccgt aagcatgtcc accacaattt
     2461 aaaatggaac ctacatgata ctccactaaa gctttgtgca atttagccgg ttcaatatga
     2521 tgtggttcta ctaaattata aataccacct tcgcaaacta catctaaatt taattgcgtc
     2581 atttgaccaa ctttttcttc aatggtcatt tttgctatta atgcatcaat atctacattc
     2641 gattgagcat gtagggtaga ggtgataaaa aaaataccaa tgctgttttt tatcttcctt
     2701 acaacattat tttgtagctg tttcattgat tattcgcatt tgaccccaca gttgaggtga
     2761 tgtgtgaaaa ccttcgttat atggattatt ccatctgtat tgtttatctc tgccttttgt
     2821 tgttccggca tcaatggcaa tttctatctg ataattattt cctgccacta atggtgcggc
     2881 tataaattgt tccatcggaa ttacagcttc tattacataa ccggttttag tggttttagt
     2941 aataactgtt cctttcgtat tggttccgct gcgccagttc cataacacag gagtggatgt
     3001 tgcacgaatt ccaaaatgct ggtcgcttaa taaaaaagct tttctgtttg ttcccgcatt
     3061 actgtttgtt gaaaatgcaa tttccaatgc atcaccgttc cagatatctt ttccgttttg
     3121 attattttgt agaggtgatg catcctgaac ttctgcaaat agcattacat ttttatcatt
     3181 tacagcaacc catacagtac cattttctaa ttgaaatccg tcacctataa aaaacgattc
     3241 tttaacacca tccatggtgg gtaaagaatt tacatacggt acatctacac ttttagatgc
     3301 tatggtttgt gtaggcacaa ttttcatttc atctaaaaat atttctcctt ccgattcgaa
     3361 ctgaatgagt aattgtttta tacccgattt atcggcatca aactgattaa aatcaaatgc
     3421 agttaatgga atttgtactt tggtccactg ttcagtaatt tttccgcctt caataaaatt
     3481 ggaaaataca cctgcccatg cctgtccgcc gccaaaatct tcaaaagcca tggcccatgg
     3541 taatccgggc tgagtgcctt taggtgaacg caccataaat tgaatggccg cctcattata
     3601 aatttccgat aaattttttc cgttccatcc atcccatcca atgcccattc ctacccaatc
     3661 gcaaccacct gatgttttat tccatttaag atataaacca ccggcgccaa tcatgctgct
     3721 gtcgaattta ttttgtaatt gcagacaagc attgttttct gttttccata attcactggt
     3781 aacataatcg ctaaaaatat tcacggcata tacatttttt atatgcggaa tactgtcgct
     3841 tttagctgta tcataaatgc taaccgattt tgttgcacac gaaaataatg ttggtgcaat
     3901 taaacctgcc aacaataatt ttatgatatt gcctgatgaa gtagtcatta tggtttaaat
     3961 ggttggtttt cggtaaataa cattaaatcc atgtaagctt gtgcatatcc tgaaaccgga
     4021 ttagcaagca ttaatatgga aacttgaatt aatttttccg gttcgtaaat accattgcca
     4081 atcgggtcta caggagcgcc atttactaaa gttggcatat cagcatattt gctgctgatt
     4141 aattgccatc catcttcatt atttaatgct acttctatag aaaataaatc ttcttcacca
     4201 tcggtatata cgccatcgcc attatcatct tcgcggaatt gaaataaaac taatccattg
     4261 tttaatcccg gaattttcga taacatcaca ttaaaataaa cattattcgg attgctgttt
     4321 aatgcgaatg tagttgcacc ataagcgctg cccggaatat caattaatcc taataaccaa
     4381 tcccaatcaa ccgtgccacc catgtcgtaa tattttgaac cttgagctgc agggtctgaa
     4441 tcggtaatga taaaactcat atcagcacct gattgaacaa atgtgctcca gccgggatta
     4501 aattcacctt caaaatcgga taatattaat cctgttatca cttttgttcc taaaaccgaa
     4561 ataggtgccg ttaatgtatc atcttcacca tctatagtta actgtgcaac acaatcttca
     4621 ctgcgaaaca taggtaatgt tgttgccgaa ccatcccaaa tagcattggt ggcatctatt
     4681 aatctcgaaa aaccttcaat ggtataaact gcacctgaag tatttcctgt gatggcaatt
     4741 ttccaattta catttttcga aaattcagca gaaaaggtga gttgctgacc ggcactaaaa
     4801 tctacggttg cagcgctggc actcaggctg tctaaaatgc tgaaatcgcc ataaatatca
     4861 ttcagcgaag ggccgtcaaa accggaatca tctttggaac atgaagccag gaataaaacg
     4921 gctgaaaaca aaggaagaaa tatttttttc atatcgctta aaattgcata ctgtaaatga
     4981 aagcaaaatt gttaattgtg tatgatggta aaacatcatc aaccagatta ttgttctgcg
     5041 aaaactggtt aaattggccc atgaggtagg ttttttcaga aaaattccat cttaaacccc
     5101 cgccaatcat ttgctgactg ccatccacgg tatattcggt aaagttgata atctggtcgt
     5161 atttatccgt ttgatttaca aattcaaacc cggcataagt atgttgttgt aatcccaaaa
     5221 caatatctaa atcggaaacg atgttggcac taaatcctac acttaacaca tcggtttgta
     5281 gctctactga aggaacaccc gcttcgccat cgcgactggt attatcgctg cgcataccgg
     5341 cagttaattc aattgatttt ttccatttta ctaatttatt aatcgcgatt gttgcattgg
     5401 catccatacg cgcaaaattt ctcaaattca acgtgccttg tccgcgaatt tccgattgtg
     5461 ataaaaaaga tgcatttaat ttaaccgctt ctttacatcc cacccaattt aaacccactt
     5521 caaaaccctg acggttcgga cttgctgcac cataaggtgt aatattatcg tagcgggcat
     5581 caaaagccat taaaccattt tgtaattgta aattatataa atcagcttcg cgcaataaat
     5641 caaacattgt tgtttcgcgt aaaatctgat cgttggtaat acgttcgtat gcagcaggag
     5701 tgccatcgaa attaatgcgt ttagtttgcg cacctgcact tctgaaatca gcaccaacac
     5761 ttttcattcc aatataaata cctaattgcc aaggcgtata ttttgcttca attttggtgt
     5821 caaaaaaatt gccatctaaa actggagcaa gactgtcgtt tttaatttca ttatgcgatt
     5881 ttcccatttc accatttgcc gttaacgcaa tatcgccaaa tgtataagcc acctgcgcat
     5941 tacctgtaac taccggattt acatactgtt cagtagtgct ggctgttccc ggaaaatcat
     6001 aaaaattcgc ataatttacg cccagcgata catatttact ttgtataagc gtcatattcg
     6061 cagctgaaaa taatcgttcg ggctgattgc caccgtttgt tgctgcaacg cgaccggtaa
     6121 atacattcca gttaatttct tgaacgtatt tattaaatac aacaccaaaa tctacagcag
     6181 caccttgttg actccaggtg ttatcaccta aataaaaatt atcgtaagat acctggtcta
     6241 ataaatgcga aaaagcaccg ggtgtataat tacttaattc actgtcgtta ttataaaaag
     6301 tatacggtgt taatttataa tgcacatcac caacctgata acggacaaca tctttaacta
     6361 caccttttac atataattcg cgcacatcga aagtaacacc tgagccccag aaaccaccaa
     6421 aatcgttgcg aattcgcaca ctgctttgaa tagcaatatt atctcccggg cgaattaaca
     6481 tcccaaggtc caccaatgta tgcgacgata aacttttcgg cgcggtaatg gtgtcggcaa
     6541 cctcaacatc ttgcgacagg ttgtctaaaa acgataaggt gcgtgccgta ccgtttaaac
     6601 tgaatttttt tacttcagtc tgggcttgta tcgttaatgc cgaacacaaa atcaaacctg
     6661 taaaaatggt tgatgttatt tttttcacta gaaaaatatt tttaattcaa ctatagtttc
     6721 ctgatttcta aaagtatcgt ccgcaaaact ggcatcacta aaattagccc agcgattgcg
     6781 gatatataaa atggcattac gccctaaatc aatatctaat cccgctccat aaccattgcc
     6841 tgtttggttg cgcggacggc gggtaacctc atcaatatca gttaaataat tcccgatagt
     6901 gcgctcataa ccataataac cgcttaatac taaaccatcg gttatgctgt aataaatttc
     6961 aaattcactt gcatattgtc tgatatatgc ctcttcattt aataccggaa ttacactcca
     7021 gtcgcgttgt gctgtgctgt aatagcctaa ataaaaaata tagagcggac gattaaatgc
     7081 tactgtttta aattttattt gcggctcaaa tacggaaaaa tattttttat ttacagccac
     7141 acctgaacta tcatcagtta aattaacagt ttcataaacg tcgcgataaa taacagaata
     7201 tctgtcgtaa ggtccaacgt ttgatggaaa agcaaaacgc cagaaacgtg cacgtgttaa
     7261 actattaatt ggatgagaat acgtaattac attcgacatc ggtgtaattt ctccattcat
     7321 accaaaaccg atattaaatg aaatattatc ccatgtttgt tcaatattta aatctaatcc
     7381 tttgcggtta ttcgacatca tacccatgcg caacatcgca ctacccgttg gtcgcaacac
     7441 agcgctgctg ccaattgaac ctgctggaat atcgtttgtt gtatattccg taacgctgct
     7501 atttaaaaat accgcgttgt tattaaaaac ttcaggagca atactaaatc cgtgtaaact
     7561 aattaccgga atattttttt tgatgggaga tgtaattgtt ccgctcaaca tttggcccca
     7621 gccttcgttg tgaagaggac taaaatactg agccattccc gcttctacat gcaagccaaa
     7681 tgattttatt ttttcgttta cctcaaaagt gagcgtatta aatcccactt tttccaatgc
     7741 taagctatcg gcgtaagtaa tactgctgat ggtgttgatt ccataatatc tgccattatt
     7801 taattgttta acaagttgtc ctccgtacga catattaggt gtgctgctaa agccaccgtt
     7861 caattctgtt ttacctatta atatttttgc ttttaagtcg ccgggtaatt cagcgccttc
     7921 taagacaaaa cctttaaaag cgcgattacc ccaacgcaca tcctggtcaa ccgcaccttc
     7981 attataatat ttat
//
LOCUS       DAORKW010000112         7965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_262_length_7965_cov_4.985714, whole genome shotgun sequence.
ACCESSION   DAORKW010000112 DAORKW010000000
VERSION     DAORKW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_262_length_7965_cov_4.985714"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /gene="ftsZ"
                     /locus_tag="PLB46_06200"
     CDS             complement(<1..1254)
                     /gene="ftsZ"
                     /locus_tag="PLB46_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRF76143.1"
                     /translation="MYFDIPKEQSSIIKVIGVGGGGSNAVNHMFMQGIKDVNFVICNT
                     DQQALEMSPVPNKIQLGPSLTKGRGAGSHPSVGQQAAMESMQEIKSLLEKNTEMVFIT
                     AGMGGGTGTGGAPVVAKLAKEMGILTIGIVTVPFGFEGKRRRTQAHEGLETLKHFVDA
                     ILIVSNDKLREMFGNLAWNEAFAKADDILTTAAKGIAEIITVPGYVNVDFEDVKTVLR
                     DSGLAIMGSGSAEGENRALEAVSVALESPLLNDNDIRGARNILLNISSGSKAVLMDEI
                     ASITDYVQEAAGNDCDIIWGNCTDETLGEKIMVTVIATGFETIEQRNTRIKKNTYKIT
                     HVDTNEITAMKQNPVAQTTAVVEEVATKEPEPEIKLVNKNEVKLEKKEVKKEEDNNPR
                     QFAFNFDMFAPVEDHREEKVAEPVAP"
     gene            complement(1326..2729)
                     /gene="ftsA"
                     /locus_tag="PLB46_06205"
     CDS             complement(1326..2729)
                     /gene="ftsA"
                     /locus_tag="PLB46_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRF76144.1"
                     /translation="MSRNNRIITGLDIGTTKICAIVGQITDNGKIEVLGMGKADSFGV
                     MRGVVANIDKTVEAIRIAVAEAEQKSGVTIREVYVGIAGQHIKSLQHRDILTRNDAEN
                     EISMEDIDRMVENMHRLVLPPGDRIIHVLPQEFIVDGELGIKDPIGMSGVRLEANFHI
                     ITGQISAAKNIFKCVEKAGLKVTDLILEPLASSASVLSEEEKEAGVALVDIGGGTTDI
                     AIFQDGIIRHTAVIPLGGNIITEDIKEGCMIMKNQAEILKVKFGCALATETEDNEIIS
                     ITGLKGREPKEIKVENLARIIQARMEEILEHVYYEIKLSGFHKKLIGGIVVTGGGAQL
                     KHIVQLVSYITGMDARVGFPTEHLSKSKSEDIRHPMYATAVGLILKGMEDRNFREQVV
                     IEQQNTTTEVPVMQQEPVTVNEMQHNNNEDAFETANETQEEHKPEKNKSEKPGWLKNM
                     LSSTKKWFEEDDVSDFK"
     gene            complement(2716..3513)
                     /locus_tag="PLB46_06210"
     CDS             complement(2716..3513)
                     /locus_tag="PLB46_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76145.1"
                     /translation="MSWKKKVVRIFSLITLVACVTGLVLLTSFNAKKQDDVVCKNVDI
                     SVDQESGMFFLDNEDVKSILLHQFGDSVKGDQLKKIDVGRIEKVLENDPYVHDAETWL
                     DANGDLHIKIVQKQPIARVINKYGVNYYINENANKIPISSKFTTRVPVVTGNIQEGTY
                     NSNMIETPVLKNVLTITRFIHNNTFWNAQIEQVSVADNGSFVLIPKLGDHKIEFGGID
                     NMEEKFHKLEIFYAEGLSYTGWDKYETIKLDYKGQIVCEKKINYEQE"
     gene            complement(3510..4856)
                     /gene="murC"
                     /locus_tag="PLB46_06215"
     CDS             complement(3510..4856)
                     /gene="murC"
                     /locus_tag="PLB46_06215"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRF76146.1"
                     /translation="MQLNNIKRVYFVGIGGIGMSALARYFKYLGAEVSGYDRTRTALT
                     QQLEQEGMFIHYEDSVALIDRQADIVIYTPAIPKHHQELNYFIDHQYPLYKRSEVLGM
                     ISRERFTIAVSGSHGKTTVSGMITWILKDSGYDCTAFLGGICTNFHSNFVIGDNDVLV
                     AEADEYDRSFMRLFPNITVVTAVDSDHLEIYGTQAEVEKAFIEFGNKTEAGGLMVVKS
                     TLPIIPHLREKVWRYSLNDTTADLYAKHYDITLDGSIVTLSNDIRFTLQYPGIHNIEN
                     AVAAVSVALQLGIAPEKISSALNNFKGIYRRFEKIYSDANTVYIDDYAHHPEEIKMFL
                     KSVREIYKGKKITAIFQPHLFTRTRDLADGFAASLDLADEVILLPIYPAREEPIEGVT
                     ADTILSRVKNANKQILSKEALLEKVKNAHFDVICTIGAGDIDKLVQPIAEILKSKA"
     gene            complement(4860..5966)
                     /gene="murG"
                     /locus_tag="PLB46_06220"
     CDS             complement(4860..5966)
                     /gene="murG"
                     /locus_tag="PLB46_06220"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRF76147.1"
                     /translation="MQKAINILISGGGTGGHIFPAIAIANEIKRRRPDANILFVGAEN
                     KMEMEKVPKAGYRIEGLWISGFHRGLDMRNLSFPFKLIASYFKAGSIVKSFRPDVAIG
                     TGGFASGPSLNAAMRNGVPAIIQEQNSFPGVTNKILSRKAKTVCVAYDGMDKFFPKEK
                     VVKTGNPVRQNIVLCNVKPEKARKDFDLDLDSKILLIVGGSLGSRTLNNCLMGGLDYF
                     KEQKIQVIWQTGTLMFESCKQAARGYDNVKVFDFISNIDHAYAAADVIVSRAGAIAIS
                     ELCLVGKPVILVPFPAAAEDHQTKNAMALVEGNAAIHISDKHAQEQLIPAALQLLNDE
                     NKRTTLSNNIKAFAIEDAAERIVDEVFKLIPGVN"
     gene            complement(5966..7234)
                     /locus_tag="PLB46_06225"
     CDS             complement(5966..7234)
                     /locus_tag="PLB46_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRF76148.1"
                     /translation="MSKPVTFLLSKVKGDRYIWLVVFVLSVVSLLAVYSSTETLAYKS
                     AGGNTEYYLIKHFIIMVFGILLMYLSHLIPYKYYSRIAQILLWLSVPALIYTMTIAPE
                     VNDASRWITLPVINLTFQTSDLARFALIMYTARVLSKKQEKIESFKEAFIPVILPIAI
                     ICALILPENLSTASVLFFSCIVLMFVGRVKFKYIMLTVGAGIVLLGIIIGLSYLLPDV
                     GRLGTWNSRVESFINGGTEDDSFQTDQAKIAIAKGMIFGVGPGNSTQKDFLPYPYADF
                     IYATIIEEYGMIGGIFIIFLYLVFLYRCIKIVAKAPSSFGALLAVGLGLVLTIQAFVN
                     MGVAVHLLPTTGLTLPLVSMGGTSLWFTSLSIGIILSVSREIEMKEEEEINQAEGMEE
                     IAENVMEEDEIVLEDMAVDAISTTNKKTKA"
     gene            complement(7253..>7965)
                     /locus_tag="PLB46_06230"
     CDS             complement(7253..>7965)
                     /locus_tag="PLB46_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRF76149.1"
                     /translation="ADDPVLSDEMSKRTSPFLQNAANILTFTLDDRPHEGAWVADNSI
                     HIQFKQNLFEMTINELALQGKHNQYNTMAAGISGKLLGLNNENIRESFSDFTTLEHRL
                     EFVAQVHGIEFINDSKATNVNSTWYALESQTKPVIWIVGGIDKGNDYSLITDLVKKKV
                     KAIVCLGKDNTRIQQAFSKLVDIVVNTESMRDCVEMAYRLGNNGDVVLLSPACASFDL
                     FENFEDRGNQFKARVREL"
BASE COUNT         2312 a         1729 c         1265 g         2659 t
ORIGIN      
        1 tggagctacc ggttcagcaa ctttttcttc acgatgatct tcaaccggag caaacatgtc
       61 gaaattaaaa gcgaactgac gaggattatt atcttcctct ttcttcactt cttttttttc
      121 gagttttact tcatttttat ttaccagttt aatttctggt tcaggttctt ttgttgctac
      181 ttcttctacc actgctgttg tttgcgcaac aggattttgt ttcatggcag taatttcatt
      241 ggtatcaaca tgcgtaattt tataggtgtt ctttttgatg cgggtattgc gttgttcgat
      301 agtttcgaaa ccagttgcaa ttaccgttac cataattttt tcacctaatg tttcatcggt
      361 gcaattaccc caaataatat cgcaatcgtt accagcagct tcctgtacat agtcggtaat
      421 cgatgcaatt tcatccatca acactgcttt tgatcctgaa gaaatattca ataaaatatt
      481 tcttgcgccg cgaatatcat tatcatttaa cagtggagat tcaagtgcaa cactaacagc
      541 ttccaatgca cgattttcac cctcagcact tcctgaaccc atgatcgcaa gtccgctatc
      601 acgcaataca gttttcacat cttcaaaatc cacgttcaca taacccggaa cggtaataat
      661 ttcggcaata cctttagctg cggtagttaa aatatcatct gctttagcaa atgcttcatt
      721 ccacgccagg ttaccaaaca tttcgcgtaa tttatcattc gacacaatca aaattgcatc
      781 tacaaaatgt tttaacgttt ctaaaccttc atgcgcctgt gttcttcttc ttttaccttc
      841 aaaaccaaat ggaacggtaa caataccgat ggttaaaatg cccatttctt ttgccaattt
      901 agcaacaacc ggagcaccac ctgttcctgt tccaccgccc atacctgcgg taataaatac
      961 catttcagta tttttttcga gcagtgattt tatttcctgc atactttcca tggcagcctg
     1021 ttgacccaca ctagggtgtg atcctgcgcc acggcctttt gtaaggcttg gtcctaattg
     1081 aattttattc ggaaccgggc tcatttccaa tgcctgttga tcggtattgc aaattacaaa
     1141 gttcacatcc ttaatgccct gcatgaacat gtggtttacg gcatttgatc cgccaccacc
     1201 gacaccaatt actttaataa ttgatgattg ttcttttggt atatcgaaat acataatgtt
     1261 tgtagcgctt tttaaactag gtttacttag ttggtttata ttgactgtcc ggtcattgac
     1321 agtttttatt taaaatcaga aacgtcatct tcttcaaacc attttttagt tgagctcaac
     1381 atatttttaa gccaacccgg tttttcagat ttattttttt ccggtttatg ttcttcttgt
     1441 gtttcgttag ctgtttcaaa tgcatcttca ttattgttat gttgcatttc gttaacagtt
     1501 accggttctt gttgcatcac aggaacttca gtagtagtgt tttgttgttc gattactacc
     1561 tgttctctga aatttctgtc ctccattcct tttaaaatca gaccaaccgc agttgcatac
     1621 atcgggtgcc gaatgtcttc tgatttagat tttgaaaggt gttcggttgg gaaacccaca
     1681 cgggcatcca ttccggtgat atacgaaact aattgaacaa tatgttttaa ctgcgcacca
     1741 ccgccggtta ctacaatacc accaatcaat tttttatgga aacctgaaag tttgatttca
     1801 taatacacgt gttccaaaat ttcttccatt ctcgcctgaa taatacgtgc gagattttcc
     1861 actttaattt cttttggttc acgacctttt aatccggtga tagaaataat ttcgttgtct
     1921 tctgtttcgg tagctaatgc acatccaaat ttcactttta gaatttctgc ctgatttttc
     1981 ataatcatgc aaccttcttt aatgtcttcg gtaatgatat ttccacctaa agggataact
     2041 gcagtgtggc gaataatacc atcttgaaaa attgcgatat cagtagttcc accaccaata
     2101 tccaccaatg caacaccggc ttctttttct tcttcgctca atacggaagc agatgatgcc
     2161 aatggttcaa gaattaaatc ggtaactttt aaacccgctt tttcaacgca tttaaaaata
     2221 ttttttgctg cagaaatttg tccggtgata atatggaaat tcgcttctaa acgaacacca
     2281 ctcataccaa tcgggtcttt aattcccaat tcgccatcga caataaattc ctgaggtaat
     2341 acgtgaataa tgcggtcacc cggaggtaaa accaagcgat gcatattttc aaccatacgg
     2401 tcgatatctt ccattgaaat ctcgttctcc gcatcgttgc gggttaagat atcacggtgt
     2461 tgtaagcttt taatgtgttg cccggcaatt cccacatata cttcgcgaat ggtaacgcca
     2521 cttttttgct cagcctcagc aacagcaatt ctgatagcct caactgtttt gtcgatgttg
     2581 gcaacaacgc cacgcataac gccaaacgaa tcggcttttc ccataccaag cacttcaatt
     2641 ttaccattgt cggtaatttg tccaacaatt gcacaaattt ttgttgtgcc gatgtcaagg
     2701 cctgtaatta tccggttatt cctgctcata gtttatcttt ttttcacaaa caatttgccc
     2761 tttataatcg agtttaattg tttcatattt gtcccagcca gtataactca acccttccgc
     2821 ataaaaaatt tccagtttgt gaaatttttc ttccatattg tcaatccctc cgaactcaat
     2881 tttgtgatca cccaattttg gtatcaacac aaagcttcca ttatcagcga cggaaacttg
     2941 ttcaatctgg gcattccaga atgtgttgtt gtggatgaac cgtgttattg tcaacacgtt
     3001 tttgagcacc ggggtttcaa tcatgttcga gttataagtg ccttcttgta tgtttcctgt
     3061 aactacaggt acacgagttg taaatttact gctgataggt atcttattag cattttcatt
     3121 aatataataa ttcacaccat acttgttaat aacccttgca atcggctgtt tctgtacaat
     3181 tttgatatgt aaatcaccat ttgcatctaa ccatgtttca gcatcatgca cgtaaggatc
     3241 attttccagt actttttcga tacgaccaac atcaattttt ttcaactgat caccttttac
     3301 ggaatcacca aattgatgta acaaaattga ttttacatct tcattatcca aaaagaacat
     3361 cccactttcc tggtccaccg aaatatctac atttttgcat accacatcat cctgtttttt
     3421 ggcgttaaag gaggtcaata ataccaagcc tgtaacgcac gccaccaggg tgattagact
     3481 aaaaatgcgg actacttttt tcttccagct catgctttcg atttcagtat ttcggctata
     3541 ggttgaacta atttatcgat gtcgcctgct cctatcgtac aaatgacatc aaagtgtgca
     3601 ttttttactt tttccaataa cgcttcttta ctcagaattt gtttattcgc attttttacg
     3661 cgcgataaaa tggtatctgc tgtaacgcct tcaattggtt cttctcttgc cggataaatt
     3721 ggcaataaaa taacttcatc tgctaaatct aaacttgctg caaatccatc ggcaaggtct
     3781 cgtgttctgg taaataaatg tggttgaaaa atcgccgtga tttttttgcc tttataaatc
     3841 tccctgacac ttttcaaaaa catttttatt tcttccgggt gatgcgcata atcatcgata
     3901 taaaccgtat tagcgtcgct gtaaattttt tcaaaacggc gataaatacc tttgaaattg
     3961 tttaatgcgc tgcttatttt ttcaggggct ataccgagct gtaacgcaac agaaacggca
     4021 gctacagcat tttcaatatt atgaataccg gggtattgta aagtaaaacg aatatcgttg
     4081 ctcaatgtca caatactacc atctaaagta atgtcgtaat gtttagcata taaatctgca
     4141 gttgtatcat tgagactata acgccaaact ttttcacgta aatgcggaat aattggtaaa
     4201 gtagatttta ctaccattaa tccacctgct tccgttttat taccaaattc tataaatgct
     4261 ttttctactt ctgcctgagt gccataaatt tctaaatggt cgctgtcaac agcagttact
     4321 accgtaatgt ttggaaataa tcgcataaat gaacgatcat attcatctgc ttcagcaacc
     4381 aaaacatcat tatcgccaat cacaaaattg gaatgaaaat tcgtacaaat accacctaaa
     4441 aatgcggtgc aatcatatcc tgaatctttt aaaatccagg taatcattcc cgaaaccgta
     4501 gtttttccat gcgagccgga aaccgctatg gtaaagcgtt ctcggctaat catacccaac
     4561 acttccgaac gcttatacaa aggatattga tggtcgataa aataatttaa ttcctggtga
     4621 tgtttcggaa ttgcaggtgt ataaataacg atatcggctt gtctgtcgat taatgccaca
     4681 ctatcttcat aatgtataaa cattccttcc tgttccagtt gttgagtaag tgcagtacgt
     4741 gtacgatcat aaccggaaac ttcagcgcca agatatttaa aataccttgc caatgcgctc
     4801 ataccgatac cgccaatacc tacaaaatat acccgtttaa tattatttaa ctgcatatat
     4861 taatttacgc ccggaattaa tttaaatact tcatcaacaa ttcgttctgc tgcatcttca
     4921 atagcaaaag cttttatatt attacttaat gttgttcgtt tattttcgtc atttaacaat
     4981 tgtaatgcag cgggaataag ttgttcctgc gcatgtttat cgctaatatg tatggctgca
     5041 tttccttcca ctaatgccat tgcatttttt gtctgatggt cttcagcagc agccggaaaa
     5101 ggcaccagaa taacgggttt gcctaccaaa cacaattcag atatagcaat agctccggct
     5161 cttgaaacaa ttacatcggc agctgcataa gcatggtcga tattggaaat aaaatcaaac
     5221 acttttacat tatcataacc tctagctgct tgtttacagc tttcaaacat taatgtacca
     5281 gtctgccaaa tcacctgaat tttttgctcc ttgaaataat ctaatccacc cattaaacaa
     5341 ttattcaatg ttcgtgaacc gagactacct ccgacaatta ataaaatttt gctgtctaaa
     5401 tcaaggtcaa aatcctttct cgctttttca ggttttacat tacataaaac gatattttgt
     5461 cgtaccggat ttcctgtttt taccactttt tctttaggga aaaatttatc catgccgtca
     5521 taagcaacgc aaactgtttt tgcttttcgc gataaaattt tattcgtaac tccgggaaaa
     5581 gaattttgct cttggataat agccggaaca ccattgcgca ttgcagcgtt aagtgatggt
     5641 ccgctcgcaa acccgcctgt accaatagct acatccggtc taaatgattt cactatcgac
     5701 cctgctttaa aataactggc aattaattta aaagggaaac tcaggttgcg catatctaat
     5761 ccgcgatgaa atccgctaat ccataaacct tcaattcggt aacctgcttt aggcaccttt
     5821 tccatttcca ttttattttc agcgcccaca aataatatat tcgcatcggg gcgacgacgt
     5881 ttaatttcgt tggcaatagc aatggcagga aatatatgac ctcctgttcc accaccacta
     5941 attaatatgt ttattgcctt ttgcattagg cttttgtttt tttatttgtt gttgatattg
     6001 catctactgc catatcttct aatactattt catcctcttc cattacattt tcagcaattt
     6061 cttccattcc ttctgcctga ttgatttcct cttcttcttt catttcaatt tctctgctca
     6121 cactcaaaat aattccaata ctcaaactgg tgaaccaaag tgaagttcca cccatactca
     6181 ccaacggcaa tgttaaaccg gttgtcggca ataaatgcac ggcaacgccc atatttacaa
     6241 atgcctgaat agttaaaact aatcctaaac caacggcaag taatgcacca aaactactcg
     6301 gtgcttttgc cacaattttt atacatcgat aaagaaatac gagatataga aaaataataa
     6361 atatgcctcc tatcatgcca tactcttcaa taattgttgc ataaataaaa tcggcatacg
     6421 ggtatgggag aaaatctttt tgtgtggaat ttccagggcc aacaccaaaa atcatccctt
     6481 tagcgatggc gattttagcc tgatctgtct gaaaagaatc atcttctgtt cctccattta
     6541 taaaactttc cacacgtgaa ttccatgtcc ccaaacgacc aacatccggt aataaatagg
     6601 ataatccaat aataattccc aataaaacaa tacctgctcc aaccgtgagc atgatatatt
     6661 taaattttac tcttccaaca aacattaata caatacagga aaaaaataat acgcttgctg
     6721 tagataaatt ttccggtaat atcaatgcac aaataatagc tataggtaat atcaccggga
     6781 taaacgcttc tttaaaagat tcaatttttt cctgtttttt tgacaacacc cttgccgtat
     6841 acataatcaa tgcaaacctt gccaaatcgg atgtttggaa ggtaagatta attacaggaa
     6901 gtgtaatcca ccgtgaagca tcattaactt ccggagcaat agtcattgta taaattaacg
     6961 caggaacact caaccataac aaaatttgcg caatacgact ataatattta tatgggataa
     7021 ggtgactgag atacatcaac aaaataccaa acaccataat aataaagtgt ttgattaaat
     7081 aatactctgt atttccaccg gccgatttat atgccagtgt ttctgttgag ctatagacag
     7141 ccagcagcga aaccactgaa agtacaaaaa caaccagcca tatataacgg tcgcctttga
     7201 ctttacttaa taaaaatgtt accggtttcg acatcttacc tttttgtgaa tattataatt
     7261 cgcgaacgcg cgctttaaat tgattacctc tgtcttcaaa attttcaaat aaatcaaatg
     7321 aagcacaagc aggtgataat aaaacaacat caccattgtt gccaaggcga taagccattt
     7381 caacacaatc gcgcatgctt tcagtattta ccacaatatc taccagtttg ctaaatgcct
     7441 gttgtatgcg ggtattatct tttccaagac aaacaatagc tttcactttc ttttttacta
     7501 aatcagtaat aagcgaataa tcgttgcctt tgtcgatacc accaacaatc cagattaccg
     7561 gtttagtttg gctttcgagt gcataccatg tgctgtttac attggtagct tttgaatcgt
     7621 taataaattc gatgccatgt acctgagcaa caaattctaa acgatgttcc agtgttgtaa
     7681 aatccgaaaa actctcgcga atgttttcgt tgttcaaccc aagtaatttt ccactgatgc
     7741 ctgcggccat cgtgttgtac tggttgtgtt ttccttgtag cgctaattcg ttaatggtca
     7801 tttcaaaaag gttttgttta aattgaatat gtatggaatt atcagccacc catgcacctt
     7861 catgcggtcg gtcatccagc gtgaaagtga gtatgttggc tgcgttttgt aaaaaaggtg
     7921 aggtgcgttt tgacatttca tccgatagta cagggtcatc tgcgc
//
LOCUS       DAORKW010000113         7876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_271_length_7876_cov_5.160210, whole genome shotgun sequence.
ACCESSION   DAORKW010000113 DAORKW010000000
VERSION     DAORKW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7876
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_271_length_7876_cov_5.160210"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..82
                     /gene="rpmG"
                     /locus_tag="PLB46_06235"
     CDS             <1..82
                     /gene="rpmG"
                     /locus_tag="PLB46_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRF76150.1"
                     /translation="RNTPDRIEMKKYNPNLKKVTVHKEIK"
     gene            100..282
                     /locus_tag="PLB46_06240"
     CDS             100..282
                     /locus_tag="PLB46_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HRF76151.1"
                     /translation="MGKISKNARVSGGAKAKVAGGGKEMVKVIVTEKSPKTGAYIFKE
                     RIVHKDKVQDVLKGNA"
     gene            416..1366
                     /gene="ftsY"
                     /locus_tag="PLB46_06245"
     CDS             416..1366
                     /gene="ftsY"
                     /locus_tag="PLB46_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRF76152.1"
                     /translation="MGLFDIFKKEQKEDLNKGLEKTKQSIFSRISKAIAGKSTVDDDF
                     LDELENILISSDVGVDTTVKIIDRLNKRVSREKYFGSEELNTILKDEIVTLLSENNTP
                     DYTEFSIPVNAKPYIMLIVGVNGVGKTTTIGKLAYQFKQAGKKVLLGAADTFRAAAVD
                     QLTIWSERVDVPIIKQQMGADPAAVAFDTVNAAKSYDADVVLIDTAGRLHNKVNLMNE
                     LSKIKRVIQKVYPDAPHDVLLVLDASTGQNAFEQAKQFTAATDVTSIALTKLDGTAKG
                     GVVIGIADQFKIPVKYIGVGEKMNQLQVFNKKEFVNALFN"
     gene            1384..2694
                     /gene="rimO"
                     /locus_tag="PLB46_06250"
     CDS             1384..2694
                     /gene="rimO"
                     /locus_tag="PLB46_06250"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRF76153.1"
                     /translation="MKARTLKKDKVNIVTLGCSKNTVDSEVLLSQLRANDVEAHHQRE
                     KDDCNIIIVNTCGFIDNAKKESIDTILNYADAKASGEIDKLYVTGCLSHRYMDELQLE
                     IPEVDAWFGTLDLPKMLQKFNADYKHELIGERSISTPSHYAYLKISEGCNRTCSFCAI
                     PLMRGNHVSKSIEDVIKEAKYLASIGVKEIMLIAQELTYYGLDIYKKRALADLLYELN
                     KVDGIEWIRLHYAYPSKFPMEIIDAIKNCERVCNYLDMPLQHASDKMLKAMNRQITRA
                     ETTQLIQDIRTQIPDIGLRTTMLVGFPGETEQDIEELIAFIKIMRFERLGVFTYSHEE
                     GTSGYLLADTVSDEEKAERASRIMEVQQQISWEKNQEKVGKVYKVIIDSKEGGNFIGR
                     TEFDSPEVDNEVLIDASQHYLRIGDFANIKITRAEEFDLFGEPV"
     gene            2718..4313
                     /gene="bshC"
                     /locus_tag="PLB46_06255"
     CDS             2718..4313
                     /gene="bshC"
                     /locus_tag="PLB46_06255"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HRF76154.1"
                     /translation="MQKLSTPFIQTGRFSKIITDYLNADNALSPYYTYAPDIKNFELI
                     IKGKQAEFLNRAELVDVLEKQYANTTTSSQVKNNIQAFGKTNTFCIVTAHQLNIFTGP
                     LYVIYKTVSAIKLCSTLQQQFPQYQFVPVFWLGSEDHDFEEINHTSVFGKPFAWEDKQ
                     GGACGEYSTNSIAELCNTLFEAIGDGPHAASLKNIFTDAYLQGHNLAHATRVLLNSLF
                     QDYGLVVVDGNDTQLKANCAAIMQDELFNQSSEKIVNEVLATFPYEAQAKPRNVNLFY
                     LQPNLRERIIYHSDKDIFEINNTTITFSPEQLKKEILSHPENFSPNVILRPLFQQKVL
                     PSLAYIGGGGEIAYWLQLKSLFEYHHIQFPMLLLRDSFLLVDEAAQKKLNKLEISVDH
                     LFGEEQHVIAEYVKTHSSDAINLDAEKTAISELFTGIATKATSIDTSLDKAVMAEQQA
                     VMTSFAKLEAKLLKAEKAKMEVEINQIKGLLTKLFPNGGLQERSENFTAWYLKLGPDF
                     IKMLLENAQQPVAGFTSILVSSN"
     gene            4438..>7876
                     /locus_tag="PLB46_06260"
     CDS             4438..>7876
                     /locus_tag="PLB46_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF76155.1"
                     /translation="MELVQYPVVLVDGQYRLVHFNTAFASLLQEIDAVFPPKDLMEVF
                     PDFLQGLFSAESGAKAAKFILIEELKQPATNKYYDLQVSQIDENFDHLELLSVSCIDV
                     TSKRERLQPIKELSEHHLQMLNFTNTGVIIHKNGHIVYSNKRADLLAGLPEAESLKGK
                     EIWPFVSDAFKAKVAERIKMIIDHHSPAEPLEEQFIKMDGTMLDVEVFAFPVNYNGEF
                     AIKSIFTDITGRKNAEHQLVKSRQQYYNLVENLTDVIFQTDIDAKFIYLNASWKTLTG
                     YEIADSLGKSCFDFLFHVHGNEAFLLKVKKLLLYGAQDIQHDLLLLLANKDQRFVEVS
                     LKPIFDDNNQIIGINGIIRDIHDKKITDLEVKRIQRTIKHHQQILVSLTKEESIINGN
                     FHQALEKIVKVTAETLNVSCVNVWAFNENYSVLNCLANYNNNEKKFVEGNALTIDQFP
                     IYFKTLLRDRIIVADDAINDPSVNEFNELYNIPHQIYSMIDVAISKGEQIWGVICVES
                     RGKYYKWSLEDQSFARSIADFISLAHKSSLLSQTQHELIKRENLYRTLIEQASDAVIM
                     LDSDDRFVEVNDAMCKLSGYSRNELLTMSAADLVPKRFIGKIPGARINSKYKDGYFGE
                     RIFVNKHGEERIAEISARIFPDGRVQGIGRDITERKRQEQALKDSEARLELALKGADL
                     GVWDFYITENKMVHNKRWAEMLGYYFENTIVNQQFWEKFIHPEDKEKAYSAFQAHLRG
                     ETPFYESTVRMLASNGEWRWILDKGKVTEWDKEGNPVRASGIHQDVTTIRDYQQQLIY
                     QRKFLQEIINATPNLIYVRNANNEFVTVNTALTQFLGISAEELLNFNGNKQAPFIEII
                     KKITERDGEVFYTHKSVLIEEQSILHPVENITYWFKTIKIPLLNDDGGFTEVLSVSMN
                     ITEIKNKEQQLSHLNDQLENKVRDRTSMLESANKELETFNYSVSHDLRTPLRSIDIFA
                     YFLDKNYSNQLDKDGLENVRQIRRSIIKMTSLIDNLLIFSKIGRSDQRYGMLDTRDIV
                     DDVLTMFRDRDELLKFKIVISDLPELYGDYQMLRQAFMNLIGNAIKYTRMRKNPVIEI
                     FGYTKNATTTIAIRDNGIGFSMDLKDKLFTAFKRLHSEEQYEGAGVGLAIVERIIKRH
                     "
BASE COUNT         2715 a         1350 c         1547 g         2264 t
ORIGIN      
        1 tcgtaataca ccggacagaa tcgaaatgaa aaaatataat ccgaacttga aaaaagttac
       61 ggttcacaaa gaaatcaaat aaacccaaaa caataaacaa tgggaaaaat atctaaaaac
      121 gcccgtgtat cgggtggtgc aaaagcaaaa gttgccggcg gtggtaaaga aatggtgaaa
      181 gttattgtta ccgaaaaatc acctaaaacc ggtgcctata tctttaaaga acgtatcgtg
      241 cacaaagaca aagtgcaaga tgtccttaaa ggcaatgcat aagcgaacta aaaaatcttt
      301 gaaaagcttc tatcaacgta gaggcttttt ttgtgcccaa ttccttcaaa accatgctta
      361 acctatgggc acaaaccttt aactttgaac acaattttaa cataatcaac ccattatggg
      421 attattcgac atttttaaga aagaacaaaa agaagacctt aacaaagggc tcgaaaaaac
      481 caaacaaagt atcttctccc gtatttctaa ggctattgcc ggaaaaagta ctgtagatga
      541 cgactttttg gacgaattgg aaaatatcct catttcttcc gatgttggtg tagatactac
      601 cgtaaaaatt atcgaccgcc tcaataaaag ggtttccaga gaaaaatatt tcgggtctga
      661 agaattgaat accatcctaa aggacgaaat tgttaccctg ctttctgaaa ataatacccc
      721 agactacacc gaattttcaa tacccgttaa tgcaaaaccc tatatcatgc ttattgtagg
      781 ggtaaatggg gtgggaaaaa caactactat cggcaaattg gcctaccaat ttaaacaggc
      841 cggtaaaaag gtgttgctcg gtgctgctga tactttccgt gcagctgctg tcgaccaact
      901 tactatatgg tcggagcggg tagatgtacc tatcattaaa caacaaatgg gtgccgaccc
      961 tgctgcggtt gctttcgata ctgtgaacgc cgcaaaatcg tatgacgccg acgtggtatt
     1021 aattgatact gcaggtcgtt tacataataa ggtaaatctg atgaacgaat tatctaaaat
     1081 taaaagagta attcaaaaag tatatcccga tgctcctcat gatgtgttat tagtgctcga
     1141 tgcatcaaca ggacaaaatg cattcgaaca agccaaacaa tttacagctg ctaccgatgt
     1201 tacctctatt gctttaacaa aattggatgg cactgcaaaa ggtggcgtag ttataggtat
     1261 tgccgaccaa tttaaaattc cggtaaaata tattggcgtt ggagaaaaaa tgaatcaatt
     1321 gcaagtgttt aataaaaagg aatttgtaaa cgcattattt aattaatttt tcacttttcg
     1381 caattgaaag caagaacatt aaaaaaagat aaggtcaata ttgtaacgct tggttgcagt
     1441 aaaaacactg tggacagcga agtattgtta tcgcaattgc gcgctaacga tgttgaagca
     1501 catcaccagc gtgaaaaaga cgactgtaat attattatcg ttaatacctg cggatttatt
     1561 gataatgcta aaaaagaatc tattgatacc attttaaatt atgccgatgc aaaggcaagt
     1621 ggtgaaatag ataaattgta tgtaaccggt tgtttgagtc atcgctatat ggatgaatta
     1681 caattggaaa tacctgaggt ggatgcctgg tttggcacac tcgacctccc gaaaatgttg
     1741 cagaaattta atgcggatta taaacatgaa ttaattggcg aacgcagtat cagcacaccc
     1801 tcacattatg cttatttaaa aatttcggaa ggttgtaacc gcacctgttc attttgtgcc
     1861 atccctttaa tgcgcggcaa tcatgtttcg aaatcaattg aggatgtaat taaagaggcg
     1921 aaatatttag catctatcgg tgtaaaagaa ataatgctga ttgctcagga actcacgtat
     1981 tacggtctcg acatttataa aaaaagagca cttgccgatt tattatacga attaaataaa
     2041 gttgatggta tcgaatggat tagattgcat tatgcatacc catctaaatt tccaatggaa
     2101 attattgatg ccattaaaaa ttgtgaacgc gtttgtaatt atctggatat gccattgcaa
     2161 cacgcttcag ataaaatgct gaaagcaatg aacaggcaaa ttaccagagc agaaacaaca
     2221 caacttattc aggatatcag aacacaaatt ccggatattg gtttgagaac aacaatgttg
     2281 gtaggattcc cgggtgaaac tgaacaggat attgaggaat taatagcatt tattaaaata
     2341 atgcgcttcg aacgattagg tgtattcact tattcgcatg aagaaggcac cagtggttat
     2401 ttattagcgg atacggtgag tgatgaggaa aaagctgaac gtgcaagccg catcatggag
     2461 gttcaacaac aaatttcctg ggaaaaaaat caggaaaaag tgggcaaggt gtataaggta
     2521 attatcgact cgaaagaagg cggtaacttt atcggaagaa ccgaattcga ctcaccggag
     2581 gtagataatg aagtgcttat tgatgcgtct caacattatt taagaatagg cgattttgcc
     2641 aatataaaaa ttacacgcgc cgaagagttt gacctttttg gggaacctgt gtaaataaaa
     2701 ttgtgttgtc caccaacatg caaaaattaa gtacgccatt tattcagaca ggccggttca
     2761 gcaaaataat tactgattat ttgaacgccg ataatgcgtt atctccttat tatacctatg
     2821 cacctgatat taaaaatttc gaattaatta ttaagggcaa acaagctgaa tttctcaatc
     2881 gtgctgaact ggttgatgtg ctggaaaaac aatatgccaa tacaacaact tcatcacagg
     2941 ttaaaaataa tatccaggca tttggtaaaa caaatacatt ttgtattgta acagcacatc
     3001 aacttaatat ttttacagga cctttatacg tgatatataa aacggtttct gccattaaac
     3061 tttgttcaac tttacaacaa caatttcctc aatatcagtt tgtgcctgtt ttttggctgg
     3121 gaagtgaaga ccatgacttt gaagaaataa atcacacctc agtatttggt aagccatttg
     3181 cctgggaaga taaacagggc ggggcttgcg gcgaatattc caccaactct attgctgaat
     3241 tatgtaacac cttattcgaa gcaattggcg atgggccaca tgcagcatca ttaaaaaaca
     3301 tatttacaga tgcttatttg cagggacata atttagcaca tgcgacaagg gttttactga
     3361 atagtttatt tcaggattat ggtttagtgg ttgtagatgg taacgacaca caattaaaag
     3421 caaactgtgc tgctattatg caggatgagt tgtttaatca atcatccgaa aaaattgtaa
     3481 atgaagtatt agctaccttc ccatacgaag cacaagctaa accgcgcaat gtaaatttat
     3541 tttatttaca acccaacttg cgtgaacgta ttatttatca ttcggataaa gatatttttg
     3601 aaatcaataa tacaacaata acattttcac cggagcaact caaaaaagaa atactatcac
     3661 atcctgaaaa ttttagtcca aatgttattt tgcgcccgtt gtttcaacaa aaagtgctgc
     3721 cttcattagc ttacattggc ggtgggggag aaatagcgta ttggttgcag ttaaaatcac
     3781 tttttgaata tcaccatatt caatttccca tgttattgtt gagagattcg tttttattgg
     3841 tagatgaagc ggctcaaaaa aaattaaata aactcgaaat ttctgtagac catttatttg
     3901 gtgaagagca acacgtaatt gccgaatatg taaaaacaca tagcagtgat gccattaatc
     3961 tggatgcaga aaaaacggca atttccgaat tgtttaccgg catcgccacg aaagcaacat
     4021 caattgatac ttctttagat aaagcagtta tggctgagca acaggccgta atgacctcat
     4081 ttgccaaact cgaagccaaa ctgctgaagg cagaaaaggc caaaatggag gtggaaatca
     4141 accagattaa agggttacta accaaacttt tccctaacgg tggcttgcag gagcgttccg
     4201 aaaactttac cgcatggtat ctaaaattag gaccggactt cattaaaatg ctgctggaaa
     4261 acgctcaaca gcctgttgcc gggttcacta gtattttggt gagtagtaac taatgccatt
     4321 ttttcctgtc acaaaacttg ttatatttat gccaaatttc gactagttta gctgaaattt
     4381 gatattccta tggcatccga aatgcattcc ctaaaattgc ccctcaagta ccaggcgttg
     4441 gaacttgtac aatacccggt ggtattggtt gacgggcaat acaggctcgt gcatttcaat
     4501 acagcctttg cttcgttgtt gcaggaaata gatgcggttt tccctcctaa agatttaatg
     4561 gaagtgttcc cggatttctt acagggatta ttttcagcag aatcaggggc aaaagccgca
     4621 aaattcatat tgattgagga gttgaagcaa ccggctacca ataaatatta cgatttacag
     4681 gtgtcacaaa ttgatgaaaa tttcgaccac ctggaattac tttcagtttc ttgtatcgat
     4741 gttacttcta aaagagagcg actgcaaccg ataaaggaat taagtgagca tcatcttcaa
     4801 atgctcaatt ttactaatac gggcgtaatt attcataaaa atggtcatat tgtttattcc
     4861 aacaaacgtg ctgatttgtt agcaggtttg ccggaagcag aaagtttaaa aggaaaagaa
     4921 atatggcctt ttgtatcaga tgcttttaag gcaaaagtgg cagagcgaat aaaaatgatc
     4981 atcgaccatc attcacctgc agaaccactg gaggagcaat ttattaaaat ggatggcaca
     5041 atgctcgatg tagaagtttt tgccttccct gttaactata atggtgaatt tgccattaaa
     5101 tccatcttca ccgatataac aggaagaaaa aatgcggaac atcagctggt aaaaagcaga
     5161 caacaatatt ataacctcgt agaaaatctt acagatgtta ttttccaaac ggatattgat
     5221 gcaaaattta tttacttaaa tgcatcatgg aaaacattaa ccggttatga aattgctgat
     5281 agccttggaa aatcatgttt cgatttttta tttcatgtgc atggtaacga agcattttta
     5341 cttaaagtaa aaaagttatt gttatacggc gcacaggata ttcagcacga cttattgtta
     5401 ttacttgcca ataaggacca gcgatttgtt gaagtgagtt taaaacccat ttttgatgat
     5461 aacaatcaaa ttattggaat aaacggcatt atcagagata tacatgataa aaaaattact
     5521 gacctggaag taaaacgaat tcagcgcacc attaaacacc atcagcaaat actcgtttca
     5581 ctcaccaaag aagagtccat tataaatggg aattttcatc aggctttaga aaaaattgtg
     5641 aaagtaactg ccgaaacttt gaatgtaagt tgtgtaaatg tttgggcatt taacgaaaat
     5701 tattcggtgt taaattgttt agcaaattat aataacaatg aaaagaaatt tgttgaaggt
     5761 aatgcgttaa cgatagacca atttccaatt tactttaaaa cattgttacg cgacaggata
     5821 attgtggctg atgatgctat caacgacccg agtgtaaatg agtttaatga actatataat
     5881 ataccgcatc aaatatattc aatgattgat gttgctatta gtaaaggtga acaaatttgg
     5941 ggcgttattt gtgtagaaag cagaggtaaa tattataaat ggtcgttgga ggatcaatct
     6001 tttgcccgtt ctattgcgga ttttatttca cttgctcata aaagtagttt gttaagtcaa
     6061 actcagcacg aattaatcaa acgtgaaaat ttataccgca cactcataga acaagcttct
     6121 gatgccgtta tcatgttaga ctccgacgat cgtttcgtgg aagttaatga tgccatgtgc
     6181 aaattgtcgg gttattcacg caatgaatta ctcacgatga gtgcggctga tttagtgcca
     6241 aaaagattta tcggcaaaat accaggagca agaattaatt caaaatataa agacggttat
     6301 ttcggcgaaa gaattttcgt gaataaacac ggcgaagaac gaattgctga aattagtgct
     6361 cgcattttcc cggacggcag ggtgcaggga atcggtagag atattacaga acgcaaacgt
     6421 caggagcaag cattaaagga cagtgaggct cgattagaat tagcattaaa aggagcagat
     6481 ttaggtgttt gggattttta tatcactgaa aataaaatgg tgcataataa acgttgggcc
     6541 gaaatgttag gttattactt cgagaatacc atcgtgaacc aacagttttg ggaaaaattt
     6601 attcatcctg aagacaagga aaaagcatat tcggcatttc aggcacattt aagaggagaa
     6661 acaccattct atgaatcaac agtccgaatg ttagctagca atggcgaatg gcgttggatt
     6721 ttagataaag gaaaagtgac cgaatgggac aaagaaggta atcctgttcg tgcatcgggt
     6781 attcatcagg atgttactac aattcgagat tatcagcaac aattaattta tcagcgtaaa
     6841 tttttgcagg aaataattaa tgctacgcca aacttaattt atgttagaaa tgcgaataat
     6901 gagtttgtaa cagttaatac agcactaaca caattcttgg gtatttcggc agaggaatta
     6961 ctcaatttta atggcaacaa gcaggcgcca tttatcgaaa ttattaaaaa aattactgaa
     7021 cgtgacgggg aagtatttta tacccataag tcggtgctca ttgaagaaca atctatttta
     7081 catccggttg aaaatataac ctattggttt aaaacaatta aaattccttt actcaacgat
     7141 gatggcgggt ttactgaagt gctttcagtt tcgatgaata ttactgaaat taaaaacaaa
     7201 gaacagcagc ttagccatct caacgaccaa ttggagaaca aggttcgcga cagaacatcc
     7261 atgttagaat ctgctaataa agaactggaa acctttaact attccgtatc gcacgattta
     7321 agaacgccgc ttcgctctat tgatattttt gcttatttcc tcgacaaaaa ttatagtaat
     7381 caattagata aagatggtct ggaaaatgtg agacaaataa ggagaagtat cataaaaatg
     7441 acttcgctta ttgataatct attgatattc tctaaaattg gtcgttctga ccaaaggtat
     7501 ggcatgctgg atacaagaga tattgttgat gatgttttaa cgatgttcag ggacagagac
     7561 gaactgctaa aattcaaaat tgtaatcagc gacctaccgg aactgtatgg cgattaccaa
     7621 atgttgcgtc aggcatttat gaatttgatt ggtaatgcta ttaaatatac gcgcatgcgc
     7681 aaaaatccgg tgattgaaat ttttggatac actaaaaatg caaccaccac catagctatt
     7741 cgcgataatg gaattggatt cagcatggac cttaaggata aattatttac tgcatttaaa
     7801 cgcctgcaca gtgaagaaca atatgaaggt gccggagtag gacttgcaat tgtggagcgt
     7861 ataatcaaac gtcaca
//
LOCUS       DAORKW010000114         7824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_273_length_7824_cov_5.737161, whole genome shotgun sequence.
ACCESSION   DAORKW010000114 DAORKW010000000
VERSION     DAORKW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_273_length_7824_cov_5.737161"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PLB46_06265"
     CDS             <1..976
                     /locus_tag="PLB46_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF76156.1"
                     /translation="ELIGKTLLEITHPADIDVSEKWLAQIYSGEIESYVAEKKYIRKN
                     GTSFWAKAVVSTMNQIGNEIVTVAIIENIEKKKLTEDALEQKNKELTQINQELEHFAY
                     VASHDLQEPLRTITSFIQILDRRYVNKLDEDAQQFMGFVVDGAKRMQTLIHDLLDYSR
                     INRYNTGYEKIDLNEIFNTVSRVLKDKIESNDALVISENLPLVYGNRIQLTQVFQNLI
                     DNAIKFKAKKRKPEIIVSVNDIKDKWELIFKDNGIGISQEYFQRIFVIFQRLHTLEEY
                     SGTGIGLAICKKIIERHGGEIWVDSKPGKGTEFHLTISKHLMRALGNN"
     gene            complement(978..1982)
                     /locus_tag="PLB46_06270"
     CDS             complement(978..1982)
                     /locus_tag="PLB46_06270"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF76157.1"
                     /translation="MKKLLVICCCILFAQLVQAQDIHFSQFYAAPMLTNPANTGNFTG
                     SVRLGLNYRDQWGSVTVPYKTFSAYADAGIQPKKAVNRFGIGVTAFTDQAGDGILSTN
                     KIYLSSAYHIGYTEKSNVRFALGLTGGFVQKTVDITKLTFNSQWNDFEFDESLSNNEN
                     STTASLDYADIGAGAQLTIMPYEGERYIIGASLMHINEPQESFLGDENKIGIKYTATF
                     GGFFATGGIASFQPQLYVASQYNATEIIAGTNVSIPINAENNSYNSMFVGAWYRHKDA
                     FWLVGGAQMDKVTVSLSYDLNLSRLRGASGSLGGFEIAAAYIFGNKVKKDPLKCPAYQ
                     "
     gene            complement(1979..5503)
                     /locus_tag="PLB46_06275"
     CDS             complement(1979..5503)
                     /locus_tag="PLB46_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF76158.1"
                     /translation="MNKRLFTILLLLMTFVSAILPLGVSAQMQSVEHAQPVKYIENKN
                     QWESNILFKTQIGFSSIYLEKNAITFDLVNAHDLTHIHHPDIDSQHAHNNIRHHAYKM
                     VFKNANPEPQIIRNLQSKEYYNYLLSNDPNNWASFVYGYSEVTYKKLYNGIDLKWYSS
                     GENIKYDFIVAPGADVDDIKINYVGTDGIFIEEGNLQIYNSVNRITETKPYAYQYRNG
                     NIVVINCEYFVEDNTVSFIFPDGYNKNLALYIDPELVFASYTGATSDNWGYTATNDDD
                     GNLYGGGIVFGAGYPTTVGAFETTYQGGEEDIGISKFSADGSTLLYSTFLGGNDSELP
                     HSMIATATGELIIMGTTGSSDFPAIAGAYDNSYNGGPYTLVDGYISFDGADIFVAKLS
                     ADGGTMMGCTYIGGTGLDGMNMASGYSTFYNYGDFARGEVMIDAANNVYVASSSNSPN
                     FPTTPGAFQTSLSGDQEGVVFKLNSSLSTLLWSSFIGGSNEDGAYSIKLNSDNEPIIA
                     GGTASTDFPSTAGTWHSTYLGGAADGWVAHIAADGSDIIACTFVGTNQYDQTFFVETD
                     DNDFIYFTGQTKGAFPVTPGVYSEANGKQFIAKINAALTTLEYSTIFGSGSSAINISP
                     SAFLVDDCENVYVSGWGGTVNQIHNPATGFTTGMTVTPDALQATTDGSDFYFFVLSKN
                     AIDLLFASYFGGNFTAEHVDGGTSRFDKGGAIYQAVCAGCGGSDNFPTTDGAYSETNG
                     SGNCNLGVIKIEFNLAGIYASAIAEPSLIGCAPFDVDFTNTSLGAVNYIWDFGDGSPT
                     STLFEPSHTYSIPGDYDVMLIAIDSNSCNIADTAYLSVIVLSDSISAEFEYTTDENCD
                     SVTATFNLVAELLPTTNFLWNFGDGTISTETNPTHVYSEPGSYTITLIIDDPTSCNGI
                     DTFTATINYLYEFNEGFEFEAVGCLPINATFTANFVGESDLLWDFGDGTTGEGASITH
                     IFDTPGEYQVSLIALNCGIYDTVTQTVIIADLPVAYFADDPKYIIAETWVTFHNLSEH
                     AVAYEWTFSDGGFTTEPNGRHYFEELTSYEVCLTAYNYNGCSDTYCRTVEAVEYGTID
                     IPTAFTPNGDGNNDILFVKGIGIDNMQLLIYNRWGELVFETTNYLEGWDGTFKGNPQP
                     MEVYVYYLIGDFADSNTFERKGNITLLR"
     gene            complement(5740..6864)
                     /locus_tag="PLB46_06280"
     CDS             complement(5740..6864)
                     /locus_tag="PLB46_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76159.1"
                     /translation="MTTKKTPNKVVKISAYSTMAGAFLTLANESQAAVIYNNIDDHTY
                     TAIGWYGLDADDNGYPDFLMQAVSAEGGNWTFASAIGALSSSGYGNDANMVVGYLGDV
                     HYYGSALNAGELIGPLSPFADNTYNRAWLASVYSGSTYGQFGNTGDRFMGFKFDIDGS
                     LHYGWARLNITLNPVTVTVKGYAYDDIPNNPIIAGDLGVSCDVPDIDGTYNIMPTSAK
                     LKWFAVDAATKYQLKYRKVGTATWTKLNVSGTQKTITGLECNVAYEWNVKSKCGDLFS
                     AFSPLQTFTTAVCKIGELSSTEIDAYPNPASDAVYLDFQGLEATSIVSISDINGKQMA
                     SDFIIEDGIMQVDIHDYPAGIYMMQIQTSTVIKTMKFVKQ"
     gene            complement(6961..>7824)
                     /gene="alr"
                     /locus_tag="PLB46_06285"
     CDS             complement(6961..>7824)
                     /gene="alr"
                     /locus_tag="PLB46_06285"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRF76160.1"
                     /translation="IMVMNPEPETFDQVKQYQLEPEIYSIELLKVFIHFIGKEESSIH
                     IKIDTGMHRLGFVENDIPELLTLLKSHQHIRVASLFSHLAGADENKHDNFSEQQITLF
                     NKIAANIQSTLGYQPLRHILNSSGISRMPHAQFDMVRLGIGLYGVDPSAVLQNTLLPV
                     GTLQTNIAQIHDVKQGESIGYSRSFIAENDMRIATINIGYADGFSRRMSNGKGMVWLH
                     QKTAPIVGRVCMDMSMIDVTHIPETKIGDTVELFGEHISINDYATWQETIPYEVMTGI
                     SQRVKRIYEFE"
BASE COUNT         2518 a         1493 c         1386 g         2427 t
ORIGIN      
        1 agaattaatc ggcaaaacat tattagaaat tacacatcca gctgatattg atgtgtcaga
       61 aaaatggttg gctcaaattt attcaggcga aatagaaagt tatgtagctg aaaaaaagta
      121 tatacgtaaa aatggcactt cgttttgggc aaaagctgtt gtttctacca tgaaccaaat
      181 tggtaatgaa atagttacag ttgccattat cgaaaatatt gaaaagaaaa aattaacgga
      241 agatgcgctt gagcaaaaaa ataaagagct cacacaaatt aatcaggagc ttgaacattt
      301 tgcatatgtg gcatctcacg atttacaaga gccgcttcgt accattacca gttttattca
      361 aattctcgat cgcagatatg tgaataagct ggatgaagat gcgcagcaat ttatgggatt
      421 tgtggttgat ggtgctaaac gtatgcaaac attgattcac gacttattag attacagccg
      481 catcaatagg tataataccg ggtatgaaaa aattgattta aatgaaatat ttaataccgt
      541 aagccgtgta ttaaaagata aaattgaatc aaacgatgca ttggtgatat ccgaaaactt
      601 gccactagtt tatggtaatc ggattcaatt aacacaagta tttcaaaatt taattgataa
      661 tgccattaag tttaaggcta aaaagagaaa gccggaaatc attgtttcag ttaacgacat
      721 caaggataaa tgggaactta tatttaaaga taacggcatt ggtataagtc aggaatattt
      781 tcaaaggata tttgtaattt ttcaacgtct gcatactttg gaagaatatt caggaacagg
      841 aattggattg gcaatttgca aaaaaattat tgaacgccat ggtggtgaaa tatgggttga
      901 ttcaaaaccc ggtaaaggaa ctgaatttca tcttactatt tcgaaacatt taatgcgtgc
      961 tttaggcaat aattaattta ttggtaagcc ggacatttga gcgggtcctt tttaacttta
     1021 ttaccaaaaa tataagctgc ggcaatttca aagcctccca gagaaccact ggcaccgcgc
     1081 aagcgtgata aatttaaatc ataactcaac gatacagtta ctttgtccat ttgcgctccg
     1141 ccaacaagcc aaaatgcgtc cttatgccga taccaagcac ctacgaacat tgaattgtaa
     1201 ctgttatttt ctgcgttaat tggtatactc acatttgtac cagcaataat ttccgttgca
     1261 ttgtattgcg aagcaacata aagttgtggc tgaaaactgg caattccacc agtcgcaaaa
     1321 aaaccaccga atgtagcagt gtattttata ccaattttat tttcatcccc taaaaaagat
     1381 tcttgtggct cattaatgtg catcaatgaa gcacctataa tataccgttc accttcatat
     1441 ggcataatgg tgagttgggc accggctcca atgtcggcgt agtctaacga agcagttgtt
     1501 gaattttcgt tattggaaag tgattcgtca aattcaaaat cattccattg cgaattaaat
     1561 gtaagtttag ttatatccac cgttttttga acaaaaccac cagttaatcc taaagcaaat
     1621 cgcacatttg atttttctgt ataaccaata tgataagcac ttgataaata gattttattt
     1681 gtacttaaaa taccatcacc agcttggtcg gtaaaagcag taacaccaat accaaatcga
     1741 ttaactgctt ttttaggttg aatacctgca tcagcataag ctgaaaaagt tttgtaaggt
     1801 acagttacgg agccccattg gtcgcgataa tttaaaccta aacgaactga gcctgtaaaa
     1861 ttacctgtgt tggcaggatt agttaacata ggagcagcgt aaaattgaga gaaatggata
     1921 tcctgcgctt gcactaattg tgcaaacaaa atacagcagc atattaccaa tagttttttc
     1981 atctcaataa agtgatatta cctttccgtt caaatgtatt gctatctgcg aaatcgccaa
     2041 ttaaataata aacataaact tccattggct gcggatttcc tttaaatgtg ccatcccaac
     2101 cttccaaata attggtggtt tcaaatacta attcacccca acgattgtaa atgagtagtt
     2161 gcatattgtc aatgccaata ccttttacaa aaagaatatc attattgcca tccccatttg
     2221 gtgtaaacgc agttgggata tcaatggtgc catattctac tgcttctact gtgcggcaat
     2281 aggtatcgga gcagccatta taattgtatg cggttaagca aacttcatag gatgttaatt
     2341 cttcaaagta atgtctgcca tttggttcag ttgtaaaacc tccatcgcta aaagtccatt
     2401 cataagcaac tgcatgttca cttaaattgt ggaaggttac ccaagtttct gcaataatat
     2461 atttagggtc atcagcaaaa tatgcgacag gtaaatctgc tataattact gtttgtgtaa
     2521 cagtatcata aattccacaa ttaagtgcaa ttaatgatac ctgatactct ccaggtgtgt
     2581 caaatatatg tgtaattgag gccccctctc cggttgtacc gtcgccgaaa tcccataata
     2641 aatcactttc accaacaaaa ttggcagtaa aggttgcatt aattggtaaa cagcctacag
     2701 cttcaaattc aaaaccttca ttaaactcat ataaataatt tatagttgca gtaaatgtat
     2761 caatcccatt gcaacttgtc gggtcgtcta taattaatgt aatggtataa gaaccgggtt
     2821 ctgaataaac atgtgttgga ttagtttcgg ttgaaatggt gccatcacca aaattccata
     2881 aaaaatttgt ggttggaagt aattccgcca ccaaattaaa tgtggctgtt acgctatcac
     2941 aattttcatc tgtagtatat tcaaattctg cgcttatact atcactcaaa acaattacag
     3001 ataagtaagc agtatctgca atgttgcatg aattagagtc tattgcaata agcataacat
     3061 cataatcacc cggaattgaa taggtatgtg atggttcaaa taaagttgaa gttggggatc
     3121 catcaccaaa atcccaaata taatttacag cccctaaact cgtattggta aaatctacat
     3181 caaaaggagc acatccaatt aatgagggtt cggcaattgc agaagcataa attccggcta
     3241 aattgaattc gatttttatc acgccaagat tacagtttcc actgccattt gtttcactat
     3301 atgcaccgtc ggttgtagga aaattatcac ttcctccgca tccggcgcat acagcctgat
     3361 aaatggctcc gcctttgtcg aaacgacttg taccaccatc aacgtgctct gcagtaaagt
     3421 tgccgccaaa ataacttgca aataataaat caatcgcatt tttcgataat acaaagaaat
     3481 aaaagtcact accatctgtt gttgcttgca atgcatcggg cgtaacggtc atgccagttg
     3541 taaatccggt tgcaggattg tgtatttgat ttactgtgcc accccatcct gaaacgtaaa
     3601 cattttcaca atcatcaact aaaaatgcac ttggtgaaat atttatcgca gatgagcctg
     3661 agccaaaaat tgttgaatat tcaagcgtag ttaatgccgc gtttattttc gcaataaatt
     3721 gtttaccatt agcttccgaa taaacaccag gtgttacagg aaatgcacct ttagtttgcc
     3781 ctgtaaaata aataaaatcg ttatcgtcgg tttcaacaaa aaaggtttga tcatattgat
     3841 ttgtaccaac aaatgtacag gcaataatat cagaaccatc agcagcaata tgagcaaccc
     3901 agccatcagc tgctccgcct aaatatgtac tatgccatgt tccggcagtc gatggaaaat
     3961 cggtacttgc agtgccaccg gcaataattg gttcattgtc tgaatttaat ttaattgaat
     4021 aagcaccatc ttcattgctg ccaccaataa atgaactcca aagcaaagtt gaaaggcttg
     4081 aatttaattt aaatacaacc ccttcctgat cgccacttaa tgatgtttga aacgcaccgg
     4141 gtgtagtagg aaaattaggc gaattactac tacttgctac atacacatta tttgctgcat
     4201 caatcataac ttcacctcgc gcaaaatcgc cataattata aaaagtggaa tatccggaag
     4261 ccatattcat accatctaaa ccggtgccac caatataggt gcaacccatc attgtgcctc
     4321 cgtcggcaga taatttggcg acaaaaatat cggcaccatc gaaactgatg tatccgtcga
     4381 caagcgtgta tgggccgcca ttataactgt tgtcataagc tccggctatt gctggaaaat
     4441 cggaagagcc ggttgtgccc ataatgatta attcacctgt agcagtggca atcatactat
     4501 gcggtaattc actgtcattg ccacctaaaa atgtactgta taacaatgtg ctgccatctg
     4561 cactgaattt tgaaatacca atgtcttctt caccaccttg atacgttgtt tcaaatgcac
     4621 ctacagtggt cggatatcct gcaccgaata caattccgcc gccatataaa ttaccatcgt
     4681 catcattagt tgctgtgtag ccccagttgt cgctggtagc gccggtatac gaagcaaaca
     4741 ccaattccgg gtcaatataa agtgctaagt ttttgttgta accatccgga aaaataaatg
     4801 ataccgtatt gtcctcaaca aaatattcgc aattaattac aacaatatta ccattgcgat
     4861 attggtaagc ataaggtttt gtttcggtga tgcgatttac tgaattataa atttgcaaat
     4921 ttccttcttc aataaaaata ccatcggttc caacataatt aattttaata tcatcaacat
     4981 cagcacccgg agctactata aaatcatatt taatattttc acctgatgag taccatttta
     5041 aatcgatacc attgtataat tttttgtaag taacctcact atacccataa acaaatgagg
     5101 cccaattatt cgggtcgttt gaaagtaaat agttataata ttctttagat tgtaaattgc
     5161 gaattatctg aggttccgga ttcgcatttt tgaaaaccat tttataggca tggtggcgaa
     5221 tgttgttgtg tgcatgttgt gagtcgatat cgggatgatg aatatgcgtt aaatcatggg
     5281 catttaccaa atcaaatgta atggcatttt tttccagata gatggatgaa aagccaattt
     5341 gagttttgaa taaaatattg gattcccact gatttttgtt ttcaatgtac ttgaccggtt
     5401 gagcgtgctc aactgactgc atttgcgctg atacgcccag aggcagtata gcagacacga
     5461 atgtcattaa gaggagcaat atggtaaata accgtttgtt cacgactttg gttttgtttg
     5521 ggggtgtgca agtgcaaaga aaacaaccaa ttgatttctt aaaaaggaca aaaaccggct
     5581 actttcgaca cttagtcact taaaaataca gtattttgct tggttctacc aaatctggcg
     5641 ccgattgtga cattaatttt aacaatggct aaggcaccaa atcggacaaa tttgcccaat
     5701 tggtgcctta gcagactgta aagccacaaa atataggatt tactgcttta caaatttcat
     5761 agttttaatg actgttgaag tttgaatttg catcatataa attcctgccg ggtagtcatg
     5821 tatatctacc tgcataatgc catcttcaat tataaaatcc gacgccattt gtttaccgtt
     5881 aatatcgcta atggaaacaa tagaagtcgc ttcaagtcct tgaaaatcaa ggtaaacggc
     5941 atcactcgcc ggatttggat acgcatcgat ttcagttgag gaaagttcac caattttaca
     6001 aacggctgtg gtaaaagttt gtaacggaga aaaagcggaa aataaatcgc cacattttga
     6061 ttttacattc cattcataag caacattaca ctccaatccg gtaattgttt tttgtgtacc
     6121 cgatacattt aacttagtcc atgtagcggt tccaactttt ctatatttta attggtattt
     6181 ggtagctgca tctactgcaa accattttaa tttggcagaa gttggcataa tattataggt
     6241 gccatcaata tcaggaacgt cgcaagaaac tccaaggtca ccggcaatta tcgggttatt
     6301 cggtatgtca tcgtaggcat atccttttac ggtaacagtt acagggttta gtgtaatatt
     6361 taatctggcc caaccataat ggaggctgcc atcaatgtca aatttaaaac ccatgaatcg
     6421 gtctccggta ttaccaaatt gaccataagt gctacctgaa tatacagatg ctaaccacgc
     6481 tctgttataa gtattatctg caaatggcga taatggccca ataagttcgc ccgcatttaa
     6541 tgcacttccg taataatgta catctcccaa ataaccaact accatatttg catcgttacc
     6601 atatccggat gaactaagcg caccaattgc tgaggcaaac gtccagttgc ctccttcggc
     6661 ggaaactgcc tgcatcagaa aatccggata tccattgtca tccgcatcta agccatacca
     6721 tcctattgcg gtgtatgtgt gatcatcgat attattgtaa atgactgcag cttgcgattc
     6781 atttgccaat gtcaaaaagg ctcctgccat tgtagaataa gctgaaattt ttacgacttt
     6841 gttaggagtt ttttttgttg tcatgagtat agtttttatt taatacaata ataaataaat
     6901 aattttaaaa tttgtgggga agtaagtaaa ttcaatttta aataatctaa aagatataga
     6961 ttattcaaac tcatatatac gttttacccg ctgcgaaatt ccggtcatta cttcgtaagg
     7021 aattgtttct tgccatgttg cataatcatt aatggaaata tgttcgccaa ataattctac
     7081 cgtatcgcca atttttgttt cgggtatatg tgttacatca atcatactca tatccataca
     7141 tacgcgacct acaataggag ctgttttttg gtgcaaccaa accattcctt ttccattgct
     7201 cattcttctg gaaaacccat cagcatatcc aatattaatg gttgcaattc gcatatcatt
     7261 ttcagcaata aacgacctgc tatagccgat actttcacct tgttttacat cgtgaatttg
     7321 tgcaatatta gtttgtaatg ttccaacagg taataatgta ttttgcagca ctgctgaagg
     7381 gtcaacaccg tataatccta tgcccagtcg caccatatca aattgtgcat gtggcattct
     7441 gctgatgccg gatgaattta aaatatgtcg cagcggttga tagcctaaag tggattgaat
     7501 attagcggca attttattaa atagtgttat ttgttgttca gagaaattat catgtttatt
     7561 ttcatcagct ccggctaaat gggaaaataa acttgcaact cgaatgtgtt gatgtgattt
     7621 aagtaatgtt aataattccg gtatgtcatt ctcaacaaaa cccaaacgat gcataccggt
     7681 atcaatttta atgtgaattg atgattcttc ttttcctata aaatgaataa aaacttttaa
     7741 taattcaata ctataaattt caggttctaa ctgatactgt tttacctggt cgaatgtttc
     7801 tggttcgggg ttcattacca taat
//
LOCUS       DAORKW010000115         7818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_274_length_7818_cov_5.273090, whole genome shotgun sequence.
ACCESSION   DAORKW010000115 DAORKW010000000
VERSION     DAORKW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_274_length_7818_cov_5.273090"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PLB46_06290"
     CDS             <1..1224
                     /locus_tag="PLB46_06290"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76161.1"
                     /translation="TTNWGLYNDGTVFKIKTDGSDFVKLLDFQDTITGAVGQSGLVSD
                     GTYLYGITKYQAAYGFGGIYKIKMDGSDFVKIYDFPGEEGGRFPEGKLFYDGEYLFGM
                     SPFGGSYSKGFIYRLKTDGSDFTNIFSFDGPSSGTGPYGALISDGTFLYGMTTYGGLL
                     NQGTAFKVKKDGTSYQKLMDMQDDPNGSLPYGSLVYDGTYLYGMTNIGGEYNYGTIFK
                     ILTDGSGYEKLYDFDLTTGAQPLGSLIIDGTTIYGMTELGGPDNDGFAFQIDNDGSNF
                     THLIDFNGDNGSLPFGSLIKVNGALFGMTAEGGANNTGVIFRYGEEEIIAVNEFNQNA
                     FHVYPNPNSGDFNIVIDAVNLNTGLLLEVINPLGQIVLSQNITTTQTQIHLSQKIPGI
                     YLIKCGTNISTVFVF"
     gene            complement(1399..3720)
                     /locus_tag="PLB46_06295"
     CDS             complement(1399..3720)
                     /locus_tag="PLB46_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HRF76162.1"
                     /translation="MKKLLTQPIRRLQHAAINCMFIASGLMATQVMASSHREAPLIAN
                     DPLADNTDLYVFRSPDDPNTVTIIANYIPAEIPFGGPNYNHFGENIRYEIHIDNNILT
                     PGDDIIYRATFTRTNEDPTTFFSIRLGAENLKTTYMLEKSIDGGITFMTIVTDGIVPA
                     INIGARSIEDAVVGLGAASYEELMMNSIATAVTGEKVFCGPVDDPFFVDLGGIFDLGN
                     SPRATETPRDGLGHFNTHSIALQIPISMLQKDGLSVDMADDILDGDFVIGVWASASRR
                     AMRTLNGDGTESVSGDWVQVSRLGMPLTNEAVIPLGEKDYWNSLSPYAEDASHFEFFY
                     NPELGLYMDDALFGTAVPQFQKLRIQKASLGAYNFSNGADGLYGLKGSPAVAGTALDD
                     AIFGTLLLPAAGKPRSVDLWPIFFTGAPNFPPYQLATGKGGNPLAVGKPFINNFLPNG
                     GDMLRLNMAVPTTPRNDPSFSSLGLVQAAVLGLTDPTYAATADLQWIPNMDGFPNGRR
                     LEDDVTRIELQAVSGIVLAAIGLWYDDYDPLVDPSPVTTDLLDVYTYTTGVESNDTTF
                     KAKFPYVQKPWSGAGKCSGLPVDYLAETECDIPTDLSAVTVDATTVNLSWSAEEATTY
                     RVDYRVVGVGPVMKAPSVANFTTLYGLTPCTNYEFRVTVKCVNAGENYTTEWVPFSTP
                     CRMGTTTENMMEVYPNPAKDKLQINYFTSEGGNVAISVVDVTGKVYITQNTNATNGNN
                     TFSLSLEQLNSGVYFVQIKNGEKQVIDKFIVTK"
     assembly_gap    4108..4207
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4575..4874
                     /locus_tag="PLB46_06300"
     CDS             4575..4874
                     /locus_tag="PLB46_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HRF76163.1"
                     /translation="MKRLKNIHPGEILSEEFLIPLEISAYRLAKEIAIPQTRISEVIK
                     GNRRITADTALRLAKFFGNSAKFWLGLQNDFDIENELREKGKIIMGIRRFDGKVA"
     gene            5494..>7818
                     /locus_tag="PLB46_06305"
     CDS             5494..>7818
                     /locus_tag="PLB46_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HRF76164.1"
                     /translation="MLDKIIAFSIKNKFIISMMTVALIVWGVWSATQLPIDAVPDITN
                     NQVQIITVCPTLAGQEVEQLVTYPIEQSIANLPDLEELRSISRFGLSVITVVFADNVD
                     IYFARQLITERLKEAESKIPVGVGTPELAPVSTGLGEVYQYIIHPKKGSESKYTPMDL
                     RTMQDWIVARQLYGTPGIAEVNSFGGQLKQYEVAVNPDRLIAMGITIPEIFTALEKNN
                     ENTGGAYIDKKPNAYFIRGVGLIGSFEDIKNIVVKTNPNGIPILIKDIAEVQFGSAVR
                     YGAMTYDGEVDAVGGVVMMLKGANSADVVNRIKDKMETIQKSLPDDIVIEPYLDRTDL
                     VNRAISTVEKNLIEGALIVIFVLVLFLGNLRAGLIVASAIPLSMLFALGLMNLFGVSA
                     NLMSLGAIDFGLIVDGAVIIVEATLHHLGLRKTIGRLSQKEMDEEVFKSASKIRSSAA
                     FGEIIILIVYIPILTLIGIEGKMFRPMAQTVGFAIFGALILSLTYIPMMCAAFLPKTI
                     SNKKTFSDRMMQFLQRNYAPLLAKAINLKNLIIGATVIIFVISLVLFSRMGGEFIPTL
                     NEGDFAFHCILPQGTSLSQSIETSMQASRIIKQFDEVKMVVGKTGSAEVPTDPMPPEA
                     TDMMIILKSPDEWKRDISYDELADEIMAKLEMIPGVFFEKNQPIQMRFNELMTGIRQD
                     VAVKIFGENMDTLLYYANKVNAVVQSVEGATEPSVERVAGLPQIVINYNRSQIANYGL
                     NIEDINHIVSTAFAGGGAGVVYENERKFDLVVRLD"
BASE COUNT         2507 a         1409 c         1472 g         2330 t
ORIGIN      
        1 accacaaatt ggggactgta taacgatggc actgtattta aaattaaaac cgatggttcc
       61 gattttgtca agctgctcga ttttcaagat actataacag gtgccgtggg acaaagcgga
      121 ctggtttcag atggcactta tttatacggc ataacaaaat atcaagccgc ttatggcttt
      181 ggaggcattt ataaaattaa aatggatggc tccgattttg ttaaaattta cgatttcccc
      241 ggtgaagaag ggggccgctt tcccgaagga aaattatttt acgacggcga atatttattc
      301 ggtatgtcac ccttcggcgg tagttattca aaaggattta tttacagatt aaaaaccgat
      361 ggctccgatt ttacaaatat ttttagcttc gacggaccct cctccggaac gggaccttat
      421 ggtgctttaa tttcagacgg cacattttta tatggcatga caacctatgg tggattatta
      481 aatcaaggca ccgcatttaa agtgaaaaaa gatggaacat cttaccaaaa attaatggac
      541 atgcaagatg acccaaatgg ttccctacct tatggtagcc tggtttatga cggaacttat
      601 ttatacggaa tgacaaatat cggcggtgaa tataattatg gtactatttt taaaatatta
      661 accgatggaa gcgggtatga aaaattatac gattttgatc ttactaccgg agcccaacca
      721 cttggctcgt taattatcga tggaactaca atttatggca tgacagaact tggtggtcct
      781 gataacgatg gatttgcttt tcagatagat aatgatggta gtaatttcac acatttaata
      841 gatttcaatg gcgataacgg ttcacttcca tttggtagtt taattaaagt aaacggtgcg
      901 ttatttggaa tgactgctga aggtggagca aataataccg gtgtgatttt tcgttacggg
      961 gaagaagaaa ttattgcagt aaatgagttc aatcaaaatg cttttcacgt atatcctaac
     1021 ccaaattccg gtgattttaa tattgtaatt gacgctgtta atttaaatac cggtttatta
     1081 ctcgaagtaa ttaacccgct cggacaaatt gtattatctc aaaacatcac taccacccaa
     1141 acccaaattc atctctcaca aaaaatacct ggtatttatt taattaaatg cgggactaat
     1201 atatctacag tatttgtttt ttaaaagcaa cctgccaaag gcagacaggc actgattaaa
     1261 cggatttaat gggtttaaac ggatttaatc tgtccttatc cgttaaatcc gtttaatctg
     1321 tgttcctttt ttaatcgtct aaaaaaaaag aggggcctaa cgacccctcc ttaatataaa
     1381 caaacataca agcacctttt atttagttac gataaattta tcgataacct gtttttcacc
     1441 atttttaatt tgaacaaaat aaacaccact gtttaattgt tctaaactaa gtgaaaaagt
     1501 attattgcca tttgttgcat tggtattttg agtaatataa actttacccg taacatcaac
     1561 tacacttatt gcaacattac caccttcact agtgaaataa ttaatttgta atttatcttt
     1621 tgcaggatta ggatatactt ccatcatatt ttcggtagta gttcccattc tgcatggtgt
     1681 gctaaatggc acccattcag ttgtataatt ttcacctgca ttcacacatt ttacagtaac
     1741 tctgaattcg taattagtgc atggtgttaa accatataac gttgtgaaat ttgcaactga
     1801 tggcgctttc attacaggac caacaccaac cacgcgataa tcaacgcgat aagtagttgc
     1861 ttcttctgca ctccagctta aattaacagt agttgcatca acagtaactg cacttaaatc
     1921 tgtaggtata tcacattcag tttcggctaa ataatcaacc ggtaaaccag aacattttcc
     1981 agcaccactc catggttttt gaacatatgg gaatttagct ttaaatgttg tatcgtttga
     2041 ttcaacacct gtagtgtatg tgtacacatc taataaatct gtagttacag gactaggatc
     2101 cactaacgga tcataatcat cataccacaa accaatagct gctaaaacaa ttccactcac
     2161 agcctgtaat tcgatgcgtg taacatcatc ttccaaacgg cgaccgttag gaaaaccatc
     2221 catgtttgga atccattgta aatctgcagt tgctgcataa gttggatctg ttaatcctaa
     2281 tactgctgct tgcactaaac ctaatgaact gaaacttgga tcatttcttg gtgttgtagg
     2341 aacagccata tttaagcgta acatatcacc accattcggt aaaaagttat tgataaaagg
     2401 tttaccaact gctaacggat taccaccttt tcctgttgcc aattgatatg gtgggaaatt
     2461 cggtgcaccg gtaaaaaata ttggccataa atccactgaa cgtggtttgc ctgcagcagg
     2521 taataataat gttccgaaaa tagcatcatc taatgcagtt cccgcaacgg caggtgaacc
     2581 ttttaaaccg tataaaccat cagcaccatt gctgaaatta taagcaccta aacttgcttt
     2641 ttgaatacgc aatttttgaa attgtggaac agcagttcca aataatgcat catccatata
     2701 caatcctaat tcaggattat agaaaaattc aaaatgtgaa gcatcttcag cgtaaggcga
     2761 taatgaattc caataatctt tttcacccag tggaattacc gcttcattgg ttaaaggcat
     2821 acctaaacga gatacctgta cccaatcacc tgaaacagat tcagtgccat caccatttaa
     2881 tgtgcgcata gcacggcgac ttgcactagc ccaaacacca atcacaaaat caccatctaa
     2941 aatgtcatca gccatatcaa ctgataaacc atctttttgt aacatagaga ttggaatttg
     3001 taatgcaata gaatgcgtat taaaatgacc taaaccatcg cgtggagttt cagtagcgcg
     3061 aggtgaattt cctaaatcga aaataccacc aaggtcaaca aaaaatggat catcaaccgg
     3121 accgcaaaaa actttttcgc cggttacagc agttgcaatg gagttcatca taagctcttc
     3181 ataagaagca gcacctaaac ccacaacagc atcttcaata gagcgtgcgc caatgttgat
     3241 agcaggaaca ataccatcgg taacaatggt cataaaagta ataccaccat caatactttt
     3301 ttcgagcata taggtagttt tcaaattttc ggcaccgagg cgaatactaa aaaatgtagt
     3361 aggatcttca ttggtgcgcg taaatgttgc gcgataaata atatcatcac caggggttaa
     3421 gatattatta tcaatgtgaa tttcgtagcg aatattttca ccgaagtggt tgtagtttgg
     3481 accaccaaac ggaatttcgg ccggaatata attcgcaata atcgtaactg tattcgggtc
     3541 atccggactc cggaatacgt aaaggtcagt gttgtcggca agcgggtcat tagcaatcag
     3601 cggagcctct ctgtggctcg atgccataac ctgcgtagcc atcaaccctg aggcaataaa
     3661 catgcaattt atcgctgcat gctgcaacct tctaattggt tgggtgagca attttttcat
     3721 agaatacatt taaggttaga aaatttattt cgctgatata cgaggggaat tggtggttgg
     3781 attggaatga ttggaagaaa aatgtaataa aaacctaatt acctaaaatt acaggtatcg
     3841 tgacctatct agaatagaat ttgttttgga taaaacagtt tggcgtttac tcctgaagtg
     3901 taaatttgcg cggcacgagc caatttacta cttcaggatt gccctaaagt gaaaattggt
     3961 gaggctttcc caaaatataa aataggcagt aatctcatat atggttggag ggctcgccct
     4021 aaagtgaaaa ttggtgaggc tttcccaaaa tgtaaaatag gcagtaatct catatatggt
     4081 tggagggctc gccctaaagt gaaaattnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnggt gaggaacgaa ccaattttct actttagggc gttcccgcgc aggtccaatc
     4261 aaactccaat tgacaattcc gagcgcggtc gggtcattcg ctgcaactcc tcgcccgctg
     4321 aaaaagcggg ctccggggtt tccgctgctg cccctaacgc gggtgaagca aaaaactgat
     4381 aataggtcat ttcaaagggc atcacttgga aaatgaataa cattccaaaa tgttggcata
     4441 taaaatgctg acctattcaa attagggaat taagacataa ataaaatttg ataaaaaaaa
     4501 taatacttac ctttggcgtt agtaacgtta tacgttagta ttaaaaaaat catgatatga
     4561 taaagccacc caaaatgaaa cgtttaaaaa atattcaccc cggagaaatc ttatctgagg
     4621 aatttttaat tcctcttgaa atatctgctt accgccttgc aaaagaaatt gccataccac
     4681 aaacacgcat ttctgaagtc ataaaaggca accgtcgaat cactgctgat actgcacttc
     4741 gcctcgccaa attttttggc aactcagcaa aattttggct agggctacaa aatgattttg
     4801 atattgaaaa tgagttgagg gagaaaggaa agattattat ggggattagg cggtttgatg
     4861 ggaaggtagc gtgataattg atcatccaat aaatcggcgt caatcaaatt cctaaggtag
     4921 gtaaactttc ctgccaatat cttaattgta catgaatgca actaattagg atgctgctgt
     4981 tattaggatt tcaggtatca taaggtcaaa aagtgcatat tctgactaat tttaaatatg
     5041 gccatatttc caaaagtttt caatacattt gcaatgtgag agtattcttc tttatattta
     5101 gttgttatat tttggcggta tcggttttac cttgtgccga caaaacggac tgtaaatatc
     5161 aggaatcaga aatatcgaat ttcgcaacca aagatcatac tgagcataaa ggcgacactg
     5221 aaaattgctc tccgttctgc atgtgtgcct gctgtgagca atccgtatcg tttatcctta
     5281 ctcctataac tttgaaaaat gtgaaagttt cttcaatcga agtttttcat gtttataaga
     5341 tctcattagt ttctgagact tttgaatcta tttggcaacc gcctaaaatt agttaataaa
     5401 ttactttacc tccgtctgtt gcggaaaaca attacctaaa gcgcaaatgc actgagggta
     5461 ttactgtatt tattcatcca actaatttac aatatgttag ataaaattat tgcattcagc
     5521 ataaaaaata aattcatcat ttcaatgatg acagttgcgt taatcgtatg gggtgtttgg
     5581 agcgcaaccc aattacctat agatgctgtt cccgatatta ccaataatca agtacaaatt
     5641 attactgttt gccctacgtt agcaggccag gaggttgagc agttagtaac atacccaata
     5701 gaacagagta ttgcaaactt gcctgactta gaagaattgc gaagtatttc ccgtttcgga
     5761 ctttcagtaa ttacagttgt atttgccgat aatgtagata tttattttgc tcgccaacta
     5821 attaccgagc ggctcaagga agccgaaagc aaaatccctg taggtgttgg aacacctgaa
     5881 ttagctcccg taagtaccgg tttaggtgaa gtttatcaat atattattca tcctaaaaaa
     5941 gggagcgaaa gtaaatacac accaatggac ttgcggacaa tgcaagactg gattgttgcc
     6001 cgacaacttt atggcacacc cggaattgca gaagtaaaca gttttggtgg acaactcaaa
     6061 caatatgaag tagcagtaaa tccagacaga ttaattgcta tgggaataac aattcctgaa
     6121 atatttacgg ctttagaaaa aaacaacgaa aatactggag gagcctatat tgacaaaaag
     6181 ccgaatgctt atttcatacg tggtgttggt ttgattggtt ctttcgaaga cattaaaaat
     6241 atagttgtaa aaacaaatcc aaatgggatt ccaatactta taaaggatat tgcagaagtg
     6301 cagttcggta gtgcagttcg atatggtgca atgacatatg acggcgaagt agatgctgtt
     6361 ggtggtgttg taatgatgtt aaaaggtgca aacagtgcag atgttgttaa tcgcattaaa
     6421 gacaaaatgg aaacaattca aaaatcacta ccggatgata ttgtaattga accatacctt
     6481 gacagaactg atttagtaaa ccgtgccatc agcactgtag aaaaaaatct gattgaagga
     6541 gcattaattg taatttttgt tttggttttg ttccttggaa atcttcgagc aggattaata
     6601 gtagcttctg ccattccgtt atcaatgtta tttgcgctag gccttatgaa tttgtttggt
     6661 gtaagcgcca atctaatgag tttgggagcc atagactttg gtttaattgt ggatggtgct
     6721 gtaattattg tagaagcaac attgcatcat ttaggtttac gaaaaacaat aggcagactt
     6781 tcacagaaag aaatggatga ggaggttttt aaatctgcct caaaaattag aagtagtgct
     6841 gcatttggtg aaattattat ccttattgta tacattccga tattaacctt aattggaata
     6901 gaagggaaaa tgttccgtcc aatggctcaa actgtaggtt ttgccatttt cggcgcatta
     6961 attttatcac ttacctatat accaatgatg tgcgcagcat ttctaccaaa aaccatttca
     7021 aacaagaaaa catttagcga tagaatgatg caatttcttc aacgtaatta tgcaccactt
     7081 ttagcaaaag ctattaacct caaaaactta attattggtg caactgtaat aatctttgtt
     7141 atttctttgg ttcttttttc aagaatgggt ggcgagttta tacctaccct taatgaaggt
     7201 gattttgcat ttcattgtat tctgccccaa ggaacttcat tgtcacaaag tattgaaaca
     7261 tcaatgcagg catcacgaat tattaagcag tttgacgaag ttaaaatggt tgttggaaaa
     7321 actggttccg ctgaagttcc aacagatccg atgcctcctg aagcaaccga tatgatgata
     7381 atattaaaat cgccagatga atggaaacgg gatatttcct atgatgaact tgctgatgaa
     7441 atcatggcaa aattagaaat gatcccaggt gtattttttg aaaaaaatca accaattcaa
     7501 atgcgattta acgaactgat gacaggtatt cgacaagatg tggcagtaaa aatttttggt
     7561 gaaaatatgg atacactttt atattatgca aacaaagtaa atgctgttgt gcaaagtgtt
     7621 gaaggtgcta ccgagccgag tgttgaaaga gtagccggtc ttccacaaat tgtaattaat
     7681 tacaaccgtt cgcagattgc taattatggt ttaaacattg aagacatcaa ccatatagtc
     7741 agcacagctt ttgcaggtgg cggtgcaggt gttgtttacg aaaacgaacg aaaatttgat
     7801 ttagttgtcc gtcttgat
//
LOCUS       DAORKW010000116         7777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_277_length_7777_cov_5.247993, whole genome shotgun sequence.
ACCESSION   DAORKW010000116 DAORKW010000000
VERSION     DAORKW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_277_length_7777_cov_5.247993"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PLB46_06310"
     CDS             <1..497
                     /locus_tag="PLB46_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRF76165.1"
                     /translation="DEDISREEILRQGLVSENDYLQMEDYTRKLFARGTEMALKQGLI
                     LVDTKYEFGILNGEVVLMDEIHTPDSSRYFYAEGYAERQSAGAHQKQLSKEFVREWLI
                     ENGFMGKEGQVVPEMTDSWIKTISDRYIELYEHVTGKKFEPVSNSVAEIENNVSTALS
                     RLSI"
     gene            complement(507..1169)
                     /locus_tag="PLB46_06315"
     CDS             complement(507..1169)
                     /locus_tag="PLB46_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76166.1"
                     /translation="MLKTISLLSCLIICTTLFSQDTFLRFEYSTGKSKFVAGDESGMV
                     DNKTYQLGLIIEHEVAGNFIVDEDHGRYYYGLRFRHLQAGSINSIYQGYESHTYDYLE
                     VPLGIQTDWISLFEHSLVLGTDFGIFGTYPLSVKGERTGPDAIAWSEEYERVWFIFGF
                     NIGGYIGLNFGDKIGIKANRFGQFPFIPITRSKPEGWTGPEYKPQLLTDGGWQFMVNY
                     KF"
     gene            complement(1258..2979)
                     /locus_tag="PLB46_06320"
     CDS             complement(1258..2979)
                     /locus_tag="PLB46_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRF76167.1"
                     /translation="MKKLLLTGSFIFTLIANSFTQIVSGDAFIKGNFVEGGINTCGAF
                     ATNIVAPFGYHPNASGSKLSFVADSDMDGWLSGTPNRCGDYIIPGSPVEGWSLQFNGV
                     TYTNTDQGCGLSNIPGDVDNYSNDGSAASVDWIGSIVGIEITQHSSIGINDLYALHEI
                     TIKNTTASTISNVYYKRNLDPDPEAVITAAGYTSINTVVSNPPLSGDALVSAVGEDYG
                     CYIALGARNADAKVSYGNFSTTDGSPEEAYNGLAGYTTSGTNINDEAIQITFLIGDIA
                     AGDSTVIAFAHIFDPTAEEAALAATDLNFVCNPPTSISTDVTTTSATLSWPAIVGAEG
                     YSIKYRPTAGGTWTPLPLAVTPSATITGLTACTNYTYKIFTTCDTSNSSTTVGTFTTE
                     CLPCTFAPTGLFTDNITTTTAKLNWSADPDAIKYKVSYGPVGGPMTTINATSNFKSIS
                     GLTPGTTYNFKVRSVCGAGINSPFSTSSVFTTLLKMGEYNPGITIYPNPANDFVNINL
                     QNLMPEPVTINIYDMVGNLVAAQTVENAETNYSTQLSTSNFASGMYILQVEQSGYKQI
                     EQIIISK"
     gene            complement(3092..4021)
                     /locus_tag="PLB46_06325"
     CDS             complement(3092..4021)
                     /locus_tag="PLB46_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76168.1"
                     /translation="MAIWILKAAIQKTISFLPFRHNINFFFQKYITKGVQLTDALFTS
                     KLHHCQQHFSAHIVHGNKKPSFTSLEIGTGWFPIVPIGLYLCGAEKIYSADIKSVLNK
                     ERLIETINKFIEYHNNGSLVKFLPAYQSDKINNIAALLVENKNLNELLEQLNIELLIG
                     DITKVDIQQLPKIDLINSNNTFEHIPPQVINAILDYFHNIIVADGVMSHFIDMSDHFA
                     HLDKSISAYNFLKFSKTQWKLIDNSIQPQNRLRICDYTTLLKNAQFKVVFQEPLIDSA
                     ITIDKVNINRDFKNNYTSDCLQVTHVLLVAKTI"
     gene            complement(4069..4141)
                     /locus_tag="PLB46_06330"
     tRNA            complement(4069..4141)
                     /locus_tag="PLB46_06330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4106..4108),aa:Arg,seq:tct)
     gene            complement(4314..5072)
                     /locus_tag="PLB46_06335"
     CDS             complement(4314..5072)
                     /locus_tag="PLB46_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76169.1"
                     /translation="MKKIFTLGLIVCAIQNLFAQAVGYQGKKFTVEVGYSPVTTLTAR
                     YFDFNLSDAHYLNESPILVKHIPKIAVEYVVLNRGSLMLRYNKWNFTTNLEYSDLANT
                     ETHLAGANSKGNMLTLGYKSFLTATPAPLGSYFGIYVTHYGYSSEYTDSEFDQLSIPD
                     EIRSYELDNAQAIGFFGSLGFKNILWDQFTLDVSLEAGIFLPIGESTTYFSGDEFGDN
                     YTFVIGETPQREHIKNTNVFFTVVPTVSFGWLAF"
     gene            complement(5080..7395)
                     /locus_tag="PLB46_06340"
     CDS             complement(5080..7395)
                     /locus_tag="PLB46_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRF76170.1"
                     /translation="MKKTLLAAVCLFNYVLATAQTPSEYQRVINVGTLPSEVYTPSSE
                     KYQSEIANIESDLSKSEQKTQQQYYLESGFSIDEMMRSGLVLYNPEYNSYLDAIADEL
                     LKGDPQLRSQVKFYLLRSPVVNAFEGAGGNIFISMGLISMLENEAELAYIMGHEIGHI
                     AREHGLDFYMEAKEIDKKSSNNSLLKKSSSFNASLVIKNLYSQALETEADEYGIQSLM
                     RTKYAADTNTLNNVFDVLKYAYLPFDNQSFPVDFFESKYYVTSKNLKLDSIKKITGEP
                     EKLAKKESFKSTHPSIGDRRKTVNEYAKNVGLSNRSAYLVSEEKFSQLRNVARYELPM
                     FYLHNHLYQDAIYAAYLGLQKDPDNLYLEKIIAKSLTGLSKFRNSKDDEVYAEKARFE
                     DYEGEQQQLYFMLWAMNDVELNVMALDYTFRIHLDHPEDPEVLPLCRSLVNDLVFYHF
                     NDEDDFLRDQTVSRDSLYKLSDIANPKLKVAPPSTKTKKITRKTTVRAKRTTTEKIKS
                     DKYKKHLLYVYNDYWEMKDFRRMWTDAVIERDLREEEAKLYKRAGIAYKNKGDTRNYF
                     WGKKLGIDTVVVVNPFYRRIDLRKDNAIEYISSEEGEINYMDVLQENADLLGVNLIVL
                     DPLKMNPEDATKFNQMNELNDWFGEQLDFGSINMPGYNQMMIDSIANELGSDYFLWTG
                     IVSLRDRQNILGPILYIGLSPFFIPLLPYGIYELIKPEYEFFYLSLVYNVKTREASVL
                     KFLFLHNNDSRAILNSHTYEMLYQLSKSSNN"
     gene            complement(7497..>7777)
                     /locus_tag="PLB46_06345"
     CDS             complement(7497..>7777)
                     /locus_tag="PLB46_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF76171.1"
                     /translation="RLHNIKPGTSYPATMVTTADHDDRVVPGHSFKYAAALQSAQAGD
                     KPVLIRIDTQAGHGAGKPLAKIIEEDADWISFTMWNVGIKHMTLKDKP"
BASE COUNT         2421 a         1374 c         1453 g         2529 t
ORIGIN      
        1 atgatgaaga tatttcgaga gaagaaattt tgcgtcaggg gttggtgagt gaaaacgatt
       61 atttgcaaat ggaggattat accagaaaat tatttgcacg tgggactgaa atggcattga
      121 aacaagggtt gattttagta gatacgaaat atgaatttgg tatattgaat ggtgaagttg
      181 ttttgatgga tgaaattcat acgccggatt cttcacgcta tttttatgca gaaggatatg
      241 cagaacgcca gtctgcagga gcacatcaaa aacaattatc caaagaattt gtacgtgaat
      301 ggttgattga aaatggattt atggggaaag aaggtcaggt tgttcctgaa atgacagata
      361 gttggattaa aacaatttca gatcgttata tagaattata cgaacacgtt acaggaaaaa
      421 aattcgaacc tgtatcaaac agtgttgcag aaatagaaaa caatgtatct actgcactaa
      481 gtaggttatc tatataagta attagcctaa aatttatagt tgaccataaa ctgccatccg
      541 ccatcagtga gtagttgcgg tttgtattcc ggtcccgtcc agccttccgg tttggacctg
      601 gtaattggaa taaatggaaa ttgtccaaaa cgattggctt tgataccaat tttatcacca
      661 aaattgagtc caatatatcc gccaatatta aagccgaaaa taaaccatac acgctcgtat
      721 tcttcagacc atgcgatagc atcgggccca gttctttctc ctttaactga taaaggataa
      781 gtaccaaata tcccgaaatc agtacccaaa acaaggctat gttcaaacaa tgaaatccag
      841 tctgtttgta tccctaaagg tacttcaagg taatcgtaag tatgactttc gtagccttga
      901 taaatactat tgatggagcc ggcttgtaaa tgtctgaatc ttaagccata ataatatctg
      961 ccatggtctt catcaacaat aaaattgccg gcaacttcgt gttcaataat taatccgagc
     1021 tgatatgttt tgttgtctac cattccggat tcatcaccgg caacaaattt agatttgcct
     1081 gtgctgtatt caaatcgtaa aaaggtatcc tgggaaaata gagttgtgca aattatcaaa
     1141 caggagagga gacttatcgt ttttaacata tgttaatttt gccgcaagtt aatatgcatt
     1201 ttttaaaata aaaaaaggcc gacaatcgcc ggcctttact ttattttatt ggatggttta
     1261 tttcgaaata attatttgtt ctatttgttt gtatccactt tgttctactt gtaaaatata
     1321 catgccggat gcaaaattac tagtgcttaa ttgagtggaa taattggttt cagcattttc
     1381 tactgtttgt gcagcaacca aattacccac catatcgtaa atattaatag ttacaggttc
     1441 aggcattaaa ttttgtaagt tgatatttac gaaatcattt gccgggtttg gatagatagt
     1501 aattcctgga ttgtattcac ccatttttaa caatgtggta aaaacggaag aggttgaaaa
     1561 tggcgagtta ataccggcac cacaaacact tcttacttta aagttataag ttgtgccagg
     1621 tgttaatcca ctgatgcttt taaaattaga agttgcgtta atagttgtca ttggtcctcc
     1681 aacagggcca taagatactt tgtattttat ggcatcagga tcagcagacc agtttaattt
     1741 ggcagtagtt gtggtaatgt tatcagtaaa taatcctgtt ggcgcaaatg tgcatggtaa
     1801 acattcggtt gtaaatgtgc caacagtagt tgatgaattg gaggtatcgc aggttgtaaa
     1861 aattttgtag gtataattgg tacaagccgt taatccggta attgttgcac ttggtgtaac
     1921 agccaatggc agtggtgtcc atgtaccacc tgcagtagga cgatatttaa tagaatatcc
     1981 ttcagcacca acaatagcag gccaacttaa agttgctgat gttgttgtta catctgttga
     2041 aattgatgtt ggagggttgc acacaaaatt gaggtctgta gccgctaaag cagcttcttc
     2101 ggcagtaggg tcaaagatgt gagcgaaagc aattactgta gaatcgccgg cggcaatatc
     2161 accaatcaga aaagttatct gaattgcttc gtcattaata ttagtgcctg aagttgtata
     2221 gccagctaac ccattataag cctcttcagg tgagccatca gtagtactaa aattaccgta
     2281 gctaacttta gcatctgcgt ttctagcacc aagagcaata taacaaccat agtcttctcc
     2341 aactgctgaa actagggcgt caccgcttag tggtggattt gatacaacag tattaatact
     2401 tgtatatcct gcagctgtaa ttacagcttc cggatccggg tctaaattgc gtttgtaata
     2461 aacattacta attgttgacg cagtggtatt tttaattgtt atttcatgca aagcatataa
     2521 atcatttatt ccaatagagc tgtgttgtgt tatttcgata cctacaatac ttccaatcca
     2581 gtctacactg gctgcagaac catcattgga ataattatca acgtcaccag gaatattgga
     2641 tagtccacag ccctgatctg tgttggtgta agtaacacca ttaaattgta aagaccaacc
     2701 ttcaacaggt gatcctggaa tgatataatc accacacctg ttaggtgttc ctgaaagcca
     2761 cccatccata tcgctatctg caacaaaact taatttagaa ccagaggcat taggatgata
     2821 accaaatggc gcaactatat ttgtagcaaa tgcgccacag gtgttaattc caccttcaac
     2881 gaaattgcct ttaataaagg catcgccgct tacaatttgc gtgaaactat ttgcaattag
     2941 tgtgaaaata aaggagccgg tgagcagtag ttttttcatg acattttatt taaggtaatg
     3001 attgtttaat gttatacaat tataaggagt aaatagtaaa tttaataagc attttccatt
     3061 tatgcaaaac ggcatttttt ttaatgcatg attatatggt ttttgctacc aatagcacat
     3121 gcgttacttg caggcaatcg ctagtataat tatttttgaa atcgcgattt atgtttacct
     3181 tatcaattgt gatagcagaa tcaattaaag gttcttgaaa cacaacttta aattgagcat
     3241 tttttaataa agttgtatag tcgcatatcc tcaaccggtt ttggggttgg atggaattgt
     3301 cgatgagttt ccattgggtt ttagaaaact ttaaaaaatt atatgcgcta attgatttat
     3361 ccagatgggc aaaatggtcg ctcatgtcga taaaatgtga cattacacca tcagcaacaa
     3421 taatattgtg aaaataatcg agtatggcat taataacttg cggaggaata tgttcgaatg
     3481 tattattaga attaatgaga tcaattttag gtaattgttg aatatcaact ttagtgatat
     3541 ccccaattag cagttcaata ttgagctgtt caagtagttc attcaggttt ttattttcca
     3601 ccagtaaagc agctatgttg ttgattttat cactttgata ggcaggtaaa aactttacta
     3661 atgaaccgtt attatgatat tcaataaatt tatttattgt ttcaattaac cgttctttat
     3721 tgagcactga ttttatgtcg gcagaataaa ttttttctgc accacataaa taaagaccaa
     3781 taggcacaat gggaaaccag cccgtcccga tttccaggct cgtgaaagat ggttttttat
     3841 taccgtgtac aatatgggcc gaaaaatgct gctggcagtg atgcagtttt gatgtaaaca
     3901 gtgcatcagt taactgcaca cctttggtaa tgtacttctg aaagaaaaag ttgatgttat
     3961 gcctgaaagg caaaaaggat attgtttttt gaatagctgc ttttaatatc caaatggcca
     4021 ttagctgtat tttgtgttaa ggggaaataa aacaatattc aaaaaaagtg acctcggcaa
     4081 gattcgaacc tgcgaccctc tgcttagaag gcagatgctc tatccactga gctacgaggc
     4141 cataaaaaaa taacattaaa aaagaccctc agcttagaag gcatccgccg gggcggacat
     4201 ccactgagct acgaggccaa tatgtaaaac acccttatct taaaagggca ttcgcctaag
     4261 cggtaataaa aacgctgtta gcgggtgcaa aggtacggaa ttaaagccaa tttttagaag
     4321 gctaaccagc caaaactgac ggtaggcaca actgtaaaaa atacattggt gtttttaata
     4381 tgctcccttt gcggcgtttc gcctatcacg aaggtgtaat tgtcgccaaa ttcatctcct
     4441 gaaaagtaag tagttgattc gccaatagga aggaaaatac ccgcttccag acttacatcc
     4501 agcgtaaact gatcccataa aatattttta aatcccaaag acccgaaaaa tccaatggcc
     4561 tgggcattgt caagttcata agagcgaatt tcatccggaa tcgataattg gtcaaattct
     4621 gaatctgtat attcagatga ataaccataa tgggtgacat aaatcccaaa ataactacct
     4681 aaaggtgcag gggttgcggt aaggaatgat ttatacccta gcgttaacat gtttcctttt
     4741 gagttggctc cggccaaatg tgtttcggta tttgctaaat cagaatattc caaattggta
     4801 gtaaaattcc atttattata gcgcaacatt aaactccctc ggtttaacac aacgtattca
     4861 acggctattt taggaatatg ttttaccaaa attggcgact catttaaata atgtgcatca
     4921 cttaaattaa aatcgaaata tcttgctgtt aaagttgtga caggcgaata acctacttca
     4981 acggtaaatt ttttgccttg ataacctaca gcttgtgcga acaggttttg tatggcgcaa
     5041 acaattaatc ctaaagtgaa tatttttttc atggtattat taattgtttg atgattttga
     5101 taattgatac aacatttcat acgtgtgcga atttaagatg gcccttgaat cattattatg
     5161 caggaataaa aatttgagca cactagcttc tcttgttttt acattatata ccaagcttag
     5221 ataaaagaac tcgtattcag gttttattaa ttcgtaaatg ccataaggta ataacggaat
     5281 aaaaaacggt gacaatccta tatacaggat tggcccaaga atattttgtc ggtcgcgaag
     5341 ggaaactata cctgtccata aaaaataatc agaacctaat tcattagcaa ttgaatcaat
     5401 catcatttgg ttgtagccag gcatattaat gctgccaaaa tccaattgtt cgccaaacca
     5461 gtcgttcagt tcattcattt ggttaaattt agttgcgtct tcaggattca ttttaagcgg
     5521 gtctaaaaca atcaaattaa cgcccagcaa atctgcattt tcctgcaaca catccatata
     5581 attaatttca ccttcctcgc tggagatata ttcaattgca ttgtcttttc ttaaatcaat
     5641 tctacggtaa aatgggttta caacaactac ggtatctatt cctaattttt taccccaaaa
     5701 ataatttcgt gtatcgcctt tgtttttata ggcaattccg gctcgtttgt agagtttagc
     5761 ttcttcttct cgtaaatcgc gttcaataac agcatcggtc cacatgcgcc ggaaatcttt
     5821 catctcccaa taatcattat acacatatag taggtgtttt ttgtatttat cggatttaat
     5881 tttttcggtg gttgtacgtt tagcgcgaac ggtagttttg cgtgttattt ttttagtttt
     5941 ggtgcttggc ggcgccactt ttaatttagg atttgcgata tctgaaagtt tataaagact
     6001 gtcgcgagaa actgtttgat cgcgcaaaaa gtcatcttcg tcgttaaaat gataaaacac
     6061 caaatcattt accagtgaac ggcataacgg cagcacctca gggtcttcag ggtggtctaa
     6121 atgtattcta aatgtgtagt ctaaagccat tacatttaat tccacatcat tcattgccca
     6181 aagcataaaa tataattgtt gctgctcacc ttcataatct tcaaaacgtg ctttttcagc
     6241 atacacttca tcatctttac tgtttctgaa ttttgataaa ccggtgagtg attttgcaat
     6301 aattttttcg agatataaat tgtcagggtc tttttgtagt ccaagatacg cagcataaat
     6361 tgcatcctga tataaatggt tatgcaaata aaacatgggc aattcgtagc gagcaacatt
     6421 gcggagctgc gaaaattttt cttctgaaac taaataagca ctgcgattac tcagtccaac
     6481 atttttggca tattcgttta ctgtttttct cctgtcacca atactgggat gggtgctttt
     6541 aaaagattct tttttagcca atttttccgg ctcgccggta atttttttaa tggaatctaa
     6601 tttcaaattt ttagatgtaa cataatattt actttcaaaa aaatctacag ggaaactttg
     6661 attgtcaaaa ggcaggtagg catattttaa tacatcaaaa acattgttta atgtgttggt
     6721 atcagcggca tatttggtgc gcatcaatga ctgtatgcca tattcatcag cttctgtttc
     6781 aagtgcttga gaatataaat ttttaattac cagtgaagca ttaaatgaag atgatttttt
     6841 taagagggaa ttattcgaac tttttttatc aatttctttg gcttccatat aaaaatcaag
     6901 tccgtgttca cgtgcaatat gtccaatttc gtgacccatt atataagcca gttcagcttc
     6961 gttttccagc atagaaatta atcccatgct aataaatata tttccaccgg caccttcgaa
     7021 agcgttcact accggtgaac gcaataaata aaattttacc tgactgcgta gttgtggatc
     7081 gccttttaat aattcgtcag caatagcatc taaataacta ttatattcgg gattgtacaa
     7141 cactaaacca ctgcgcatca tttcatctat tgaaaatccg ctttccagat aatattgttg
     7201 ttgtgttttt tgctctgatt tagataagtc tgattcaata ttggcaattt ctgattgata
     7261 tttttcgctg gaaggagtat aaacttctga aggtaatgtg cctacattaa ttacacgctg
     7321 gtattcagaa ggagtttggg cagtagcaag cacataatta aacaagcaaa ctgctgccag
     7381 tagtgttttt ttcattttga tgttatttac cctacaattt tactgatttt aaggataagt
     7441 agcatgaaaa aagcgaaggt ttttggcctt cgctttaaaa tgatatcctc taaattttat
     7501 ggcttgtctt tcaatgtcat gtgtttaata cctacattcc acatggtaaa actaatccaa
     7561 tcggcatctt cttcaattat tttagctaat ggttttcctg caccatggcc agcctgagta
     7621 tcgatgcgaa tcaacaccgg tttatcacct gcttgtgccg attgcagggc tgcagcatat
     7681 ttaaacgaat ggcccggaac aactctatcg tcgtgatcgg ctgtggtaac cattgtagct
     7741 ggatatgaag ttcccggttt aatattatgc aaacggg
//
LOCUS       DAORKW010000117         7748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_279_length_7748_cov_4.009359, whole genome shotgun sequence.
ACCESSION   DAORKW010000117 DAORKW010000000
VERSION     DAORKW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_279_length_7748_cov_4.009359"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLB46_06350"
     CDS             <1..846
                     /locus_tag="PLB46_06350"
                     /inference="COORDINATES: protein motif:HMM:NF018534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRF76172.1"
                     /translation="NEILTQLTRPDLPNFYQNAKNVPLIAWKTGTSYGRKDAWSIGYN
                     NNYTIGVWVGNFSGKGVPELSGANVATPLLFNLFNAIEKENNKEWNTMPSSIQFRYVC
                     SVTGKNPADFCDNVVLDYFIPGVSKNVICDHLVEVKVTENEKLSYCTTCLPESGYKVK
                     LYNNYSPEIISWMESKSIPITLIPAHNPDCERVFSDGAPSITSPINNMEYLIDKLDKQ
                     KMMLSANVASDVKKVFWYINDRLLTEADVKTPVFFEPEEGKIKISCSDDKGRNTDIEI
                     TVELF"
     gene            complement(1001..2389)
                     /locus_tag="PLB46_06355"
     CDS             complement(1001..2389)
                     /locus_tag="PLB46_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HRF76173.1"
                     /translation="MKKIFLILTMPVILFGSGCSGWVDDVSTSPNAPTEVTPALLLTV
                     SEVAMQSLYTGQLARTSSILVQHSAGNDFQMIDVRDYKITEQSNTNEWKTFYADALIN
                     QQELINVAGNENPYYRGIGTVLKCMTIGLATDFYGDVPYSQALKGLEASEAALNPAYD
                     SQESIIAAIQSELNGAINDLSAPATANALLPGSDDFIHNGDVAMWIKAAYMLKARYAN
                     RLSQRDATGSANDALAFITSAGLASNADNTNAIFGINGNELNPWASFNTTRANYIKMG
                     EFFIDMLETSSDPRLPLYATLDDGGGYSGVPKNSVDPTASNTGELYSSNANPLPLLTY
                     YEMKFIEAEAKLRLGDAAGAAAAHNDAVKANILATVGAADAAYEAANASETAGTITLD
                     KIMTQKYIAMFTQCEAWADYRRTGLPALTPNPDAIVDGIPVRFPTCIDERLYNSSAVV
                     IGDILAPVWWDN"
     gene            complement(2409..5582)
                     /locus_tag="PLB46_06360"
     CDS             complement(2409..5582)
                     /locus_tag="PLB46_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRF76174.1"
                     /translation="MKKLAALMLLIPFCLVSVAFAQSRTITGTITSATDNEPLPGATV
                     LVKGTTTGTITDLDGKYVLVVPQDKDVLVYTFLGFKMHEETIGNRNVINVGLEEDVLG
                     LETVVVTALGIPKEKLSLGISTQEVGGDRIASSGEENFIEGLSAKAAGVQVVGAAGTP
                     GASSKIIIRGPSTFTNENQPLVVIDGVPIDNSTNNTVAGDYPFNATLNGVSNSNRAID
                     VNPDDIESINILKGPAAAALYGSRAGSGAIIITTKRGNKAGDKAVHINFSSSLEVNQV
                     SNLPKQQMLYAQGTGGGKITNQGVLEPEGNFIEADPGPDQLWNTPDDGSAGTASSWGP
                     LISGLGLTAIDNPAEFFQTGMTYNNNLAISGGTDRGSLRLSLGNTQDNGVIPNTEYNR
                     TSVRVNSDMRIWDKFRIAADGNYINSGGTRAQNGSNLSGVMLSLMRTPTSYDLGAGYE
                     YPTGENRNYFSAYDNPYWTINNNPFTDNVNRLLGNFSAIYTPWDWATITYRIGTDSYT
                     DERKQIYSIGSNDPANAPGGQIEENTIRYNQVYSDLIATASKNLTQDVVMNVTLGNNL
                     TDIDINYLYARGRDLTIPDFYNLSNAADLYASQSTTKQRNAAFFGELGFEYQSMLFLT
                     VSGRNEWSSTYGPNQGSAFFPAVSGAFVFSEILNTNDIWSFGKLRFAYAQAGIEPQPY
                     SANTYFIPPLYTDGFTDGYSFPYLGQSGFGYSQLNTLGNPDLKPERLTGTEAGLELKF
                     WKGRIDLDMTVYQQESSDILLVKPIASTSGFSYVYDNAGAMVNKGIELTLNADIIKKK
                     NLTWNIGGNFSKNENEVTQLADGVEEIEIEAGFGDPGAYVIVGQPYGLLYGSQWAYNE
                     NGDLLIDGSSGLPIFDTVSGVIGDPYPDWLMNINTALSWKGLTVSGLLDIRKGGDIWN
                     GTNARMNRIGISEASGERDHTFVIEGVIEQADGSYVANDIEIAPEDYWGDYLGDFGAS
                     SQAIQDGGWVRLREVKLAYTFTLDPKNVIKGLSVSATGRNLWLKTDYLGVDPETSLTG
                     AGSNISGFDWFNNPGTKSYLFSVSAAF"
     gene            complement(5764..6489)
                     /locus_tag="PLB46_06365"
     CDS             complement(5764..6489)
                     /locus_tag="PLB46_06365"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HRF76175.1"
                     /translation="MFRNFLLGLLTVGLFTGSTCDPKVMEDVLNSVLETTITEQEVAS
                     GLMEALVQGATNGSDLLSKVNGYLGNPQVKIPFPPEAQKIETTLRDLGMNKMCDDVIN
                     SLNRAAENAAIEAKPILINSIRSMTITDAMDILFGANDAATEYLKKTTTTQLTAKFSP
                     IIESSLNKVGATKYWTDAVNYYNKIPLVADLNPDLEAYVTAKALEGLFYMVEQEELKI
                     RQDPAARAAEIVQKVFGYYDSNK"
     gene            6747..>7748
                     /locus_tag="PLB46_06370"
     CDS             6747..>7748
                     /locus_tag="PLB46_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HRF76176.1"
                     /translation="MKAVIISIGDEILNGTTINTNASWISTQIQPIGIDIHEVVAISD
                     NAIHILHTLETFIGKVDIILITGGLGPTKDDITKKTICSYFKSELVFHQEIYARLKAA
                     FEKRNIPFTENNRDQAMYPDNCTILDNPLGTAQGMWFEKNDTVIACMPGVPFEMKGLM
                     TNQVIPKIMKRFNFPAIVNKYFMTSGISESFLANKLEPLEAILPEHITLAYLPTPAVV
                     KLRLTARGEDENLLEQELSAISNQMRAILGAGIYAEEPIPLEQHIGNQLRMLKATLAT
                     AESITGGKVAHKITLIPGSSDYFRGAVVAYHQSVKQHLLKVSTTTLENFTPVSEETAV
                     "
BASE COUNT         2459 a         1489 c         1389 g         2411 t
ORIGIN      
        1 aatgaaattt taactcagtt aacccgacct gatttaccta acttttatca aaatgctaaa
       61 aatgttcctt tgatagcatg gaaaacagga acttcatatg gcagaaaaga tgcctggagt
      121 attggttaca acaataatta tactattgga gtttgggtgg gtaatttttc cggaaaagga
      181 gttccagagt tatccggagc aaatgtagct acgccgttgt tatttaattt atttaatgcc
      241 attgaaaaag aaaataacaa ggaatggaat actatgccat ctagtattca attcagatat
      301 gtttgttcag ttacaggtaa aaatcctgcc gatttttgtg ataatgtagt attggattat
      361 tttattcccg gcgtatcaaa aaatgtaatt tgcgatcatt tggtagaagt taaagtaact
      421 gaaaatgaaa aactcagtta ttgcaccacc tgtttaccgg agtcgggtta taaagtaaaa
      481 ctctacaaca actattcgcc cgaaattatt agctggatgg agtcgaaaag tattcccatt
      541 actttaatcc cagcacacaa tcccgattgc gagcgcgtat tttcagatgg cgcgccttct
      601 attacttctc ccattaacaa tatggaatat ttaattgata aactcgataa acaaaaaatg
      661 atgttaagtg ccaatgtagc atcagatgta aaaaaagtgt tttggtatat caacgatcgt
      721 ttattaactg aagcagatgt aaaaacaccg gtattttttg agcctgaaga aggtaaaata
      781 aaaataagtt gcagtgatga taaaggacgt aataccgata tcgaaattac tgtagaatta
      841 ttttaataca gaacaggaaa tatttaccgg ttgcctaaat tgttaaacca aaaaaattcg
      901 tgttatcatt taagccttaa aataaaatta tgggttgaat actacaaata aaaaaggctg
      961 ccaaaaatga cagccttttt aagtattggt aatttgtcaa ttaattatcc caccaaaccg
     1021 gagctaaaat atctccaata acaactgcac ttgagttgta taaacgttca tcgatacaag
     1081 ttgggaatct taccggaatt ccatcaacaa ttgcatctgg gttaggcgta agagccggta
     1141 aaccggttct tctataatca gcccaagctt cgcattgggt aaacattgcg atatattttt
     1201 gggtcatgat tttatctaaa gtaattgttc ccgcagtttc gcttgcatta gcagcttcat
     1261 aagcagcatc ggcagcacca actgtagcaa ggatattagc tttaacggca tcattatgag
     1321 ctgcagcagc gccagcagca tcacctaaac gcaatttagc ttctgcctca ataaacttca
     1381 tttcataata agttaaaagt ggcaatggat ttgcgtttga tgaatataat tcaccagtat
     1441 tagaagctgt tgggtccaca ctatttttag gcacacctga atatccacca ccatcatcta
     1501 aagttgcata aagaggtaag cgtgggtcgc tgcttgtttc taacatatca ataaagaatt
     1561 cgcccatttt gatatagtta gcacgcgtag tgttaaatga agcccaagga tttaactcat
     1621 taccattaat accaaatatt gcattagtat tatcagcgtt agaagctaat ccagcagagg
     1681 tgataaatgc taaagcatcg tttgcagaac cagtagcgtc gcgttgactt aatcggttag
     1741 catatcttgc ttttaacata taagcagctt taatccacat agcaacatca ccattatgaa
     1801 tgaagtcatc tgacccaggt aataatgcat ttgcagtagc aggtgcagat aaatcattaa
     1861 tagcaccatt taattcagac tgaatagctg caattatact ttcctgagaa tcataagcag
     1921 gatttaatgc agcttctgaa gcttccaatc cttttaatgc ttgtgaataa ggaacatcgc
     1981 cataaaaatc tgtagccaaa ccaatggtca tacattttaa tactgtgcca attcctctgt
     2041 agtacggatt ttcgttgcct gctacattga ttaattcctg ttgattgatt aatgcatctg
     2101 cataaaatgt tttccattcg ttggtattac tttgttcggt gattttataa tcgcgaacat
     2161 caatcatttg gaaatcatta ccagcagaat gttgcactaa tattgaactg gttcttgcaa
     2221 gctgacctgt atataatgat tgcatggcaa cctcagatac ggttagcaac aaagccgggg
     2281 taacttcagt tggtgcgttt ggtgatgttg aaacatcatc aacccaaccg ctacatcctg
     2341 aaccaaatag gattaccggc atggttaata ttaaaaatat ctttttcata ttaataattt
     2401 tttacgtttt agaatgctgc gcttacgcta aacaaataag atttagttcc tggattgtta
     2461 aaccaatcga aacctgagat attagaacct gcgccagtta aactagtttc agggtcaaca
     2521 cctaagtaat cggtttttaa ccataggtta cgacctgttg ctgaaacaga taaacctttg
     2581 attacatttt taggatctaa agtgaaagtg taagctaatt taacttcacg taatcttacc
     2641 caaccaccat cttgaatggc ttgagatgat gcaccaaaat caccaaggta gtcaccccaa
     2701 taatcttcag gagcaatttc gatatcgttg gctacatatg aaccatcagc ttgttcaatt
     2761 acaccttcaa ttacgaaagt atgatcgcgt tcgcctgatg cttctgaaat accaatacga
     2821 ttcatacggg cattagttcc gttccagatg tcgccacctt ttctgatatc taataaacca
     2881 cttactgtta aacctttcca agataaagca gtattgatgt tcattaacca atccggatat
     2941 ggatcaccaa ttacaccact cactgtatca aaaattggta aacctgaact accgtcaatt
     3001 aataagtcgc cattttcatt atatgcccat tgtgaaccat ataataaacc gtaaggctga
     3061 ccaacaatta cataggcacc cggatcaccg aaaccagctt caatttcaat ttcttcaaca
     3121 ccatcagcta actgtgtaac ttcattttcg tttttgctaa agttaccgcc gatattccat
     3181 gttaagtttt tctttttgat gatatcagca tttaatgtta attcaattcc tttgttaacc
     3241 attgcacctg cgttatcgta aacataacta aatcctgaag tagaggcaat tggtttaact
     3301 aataatatat cagatgattc ttgctggtaa actgtcatat ctaaatcgat acggcctttc
     3361 cagaatttta attctaaacc tgcttcagtt cctgttaagc gttcaggttt taaatcagga
     3421 ttaccaagtg tatttaattg gctgtaacca aaacctgact gacctaaata agggaagcta
     3481 taaccatctg taaaaccatc agtatacagt ggtggtatga aataagtgtt tgcactatat
     3541 ggttgtggtt cgatacctgc ttgagcatat gcaaaacgca atttaccgaa tgaccaaata
     3601 tcatttgtat ttaaaatttc gctgaataca aaagcaccac tcacagctgg gaagaacgct
     3661 gaaccttggt taggaccata agttgaagac cattcatttc ttcctgatac tgttaagaat
     3721 aacatgcttt gatattcaaa accaagttca ccaaagaacg ctgcgttgcg ttgtttagta
     3781 gttgattggc tagcatataa atctgcagca tttgataaat tgtaaaaatc aggaatagtt
     3841 aaatcacgac cgcgagcata taaataatta atgtcgatat cagttaagtt attacctaac
     3901 gtaacattca taacaacatc ttgtgttaaa tttttagagg ccgtagcaat taaatcgcta
     3961 tatacttgat tgtaacgaat ggtattttct tcaatttgtc cgcctggagc attcgcaggg
     4021 tcgtttgaac caattgaata aatttgtttg cgttcatcag tataagaatc tgttccgatt
     4081 ctataagtga tggtagccca atcccaaggt gtataaatag ctgagaaatt acctaacaaa
     4141 cgatttacat tatctgtaaa agggttgttg tttattgtcc aatagggatt atcataagca
     4201 gagaaatagt ttctgttttc acctgttgga tattcataac ctgcacctaa gtcgtaacta
     4261 gttggtgtac gcattaatga taacataaca ccagaaaggt tactaccatt ttgtgctctt
     4321 gttccaccac tgtttatgta gtttccgtca gcagcaattc taaatttatc ccaaatgcgc
     4381 atatcggaat tcacacgtac agatgttctg ttatattcag tatttggaat aacaccatta
     4441 tcctgagtat tacctaacga aaggcgtaaa gaacctctat cggtaccacc gctaattgcg
     4501 aggttattat tataagtcat acctgtttgg aaaaattccg cagggttatc aattgcagtt
     4561 aagcctaagc ccgaaatcag tggtccccag ctgcttgcag tacctgccga accatcatca
     4621 ggagtattcc acaattggtc aggacccgga tcagcttcaa taaagttacc ttctggttcg
     4681 agtacaccct gattggttat tttaccacca ccggttcctt gagcatataa catttgttgt
     4741 ttaggtaaat tgcttacctg atttacttct aatgaagaag agaaattgat atgaactgct
     4801 ttgtcgcctg ctttattacc acgttttgta gtaatgatga tagcacctga acctgcacgt
     4861 gatccgtaaa gtgcagccgc agctggtcct ttaaggatgt tgatgctttc aatatcatca
     4921 gggttaacgt caatagcacg gtttgagtta cttacaccat ttaatgtagc attaaaagga
     4981 taatcgcccg caacagtgtt gttggttgag ttgtcaatag gcacaccatc aattaccact
     5041 aatggttgat tttcgttagt gaaagttgaa ggtccacgaa taatgatttt tgaagatgct
     5101 ccgggagtac ctgcagcacc aacaacctga acacctgctg ctttagcaga aagaccttca
     5161 atgaagttct cttcaccaga gctcgcaatg cggtcaccac caacttcctg agtagaaata
     5221 cccaacgaaa gtttttcttt aggaataccc aaagcggtta caaccactgt ttcaagacct
     5281 aaaacgtcct cttcgaggcc aacgttaata acatttctgt taccaatggt ttcctcatgc
     5341 attttaaagc ctaagaaagt gtacactaaa acatctttgt cctgaggcac aaccaataca
     5401 tatttaccat ctaaatcggt aattgtacct gttgtagtcc ccttcaccag tactgttgcc
     5461 cccggtaagg gctcattgtc tgtagctgac gtgattgttc ctgtaatggt tctgctttgc
     5521 gcgaaggcaa cagataccaa acaaaaagga atcagcagca tcaaagcggc taattttttc
     5581 atagatttgg ttttgaatga atagaattga cttttaggtt aattatcaaa aaatttgtaa
     5641 aatttatgtt aattatctct aaacaacaaa cataaggcac tttttattta gttatagctg
     5701 ttttgtgcaa tttttttcaa ggtttaacac ttaagtgcag gttttcaaca cattctccat
     5761 accttattta ttggagtcgt aatacccaaa caccttttga acgatttcag ctgctcgagc
     5821 tgcaggatcc tgacgaattt ttaattcttc ctgctcaacc atataaaaca aaccttctaa
     5881 agcctttgcc gtaacatatg cctctaaatc ggggttcagg tctgctacta aaggtatttt
     5941 attataataa ttgacagcat ctgtccagta tttggttgca cctactttat tcagtgaact
     6001 ttcaataatt ggagaaaact tagcggttaa ttgagtagta gttgtttttt tcaaatattc
     6061 agtcgccgca tcattagccc caaacaatat gtccatggca tctgtaattg tcatggaacg
     6121 gatactattt attaaaatgg gtttagcctc aatagcagca ttttcggctg cacggttcaa
     6181 tgagttgatt acgtcgtcac acattttatt catgcctaaa tcacgcaatg tagtctcaat
     6241 tttttgtgct tcaggtggga atgggatttt cacctgtgga ttacctaaat agccattaac
     6301 tttagataac aagtctgaac cattagtagc accctgaact agcgcttcca tcaaaccgct
     6361 ggcaacttcc tgttccgtaa tggttgtttc tagcactgag tttaatacat cttccattac
     6421 ttttgggtcg caggtagagc ccgtaaaaag gcctacagtt agcaagccga gcaaaaaatt
     6481 tctgaacata taacttgaat ttggcaccaa gttataaatt tttttgattc aagagtttgg
     6541 caataaataa taataaaaat ggtggaattg acctggcagg ttaaacggct taaacttaat
     6601 cgttttaaga ttcaaccttt tatcaaaatt attgtctttc cttaattcgt tcaaccatta
     6661 ttacgaattt gataatacca gcaagtaaat tgtataactg ctggtaaata ttcttatgcg
     6721 gattgtattt ttacaaaaaa caaaaaatga aggctgttat tatatctata ggagatgaaa
     6781 tattaaacgg aacaacgata aacaccaatg ccagttggat ttccacgcaa attcaaccta
     6841 ttggtataga tattcatgaa gtagttgcta ttagtgataa tgccattcat attttacata
     6901 cgctggagac ttttattggt aaagttgata taatattaat aacaggtggt ttaggaccta
     6961 ccaaagatga catcaccaaa aaaaccattt gttcatattt taaaagtgag cttgtttttc
     7021 atcaggaaat ttatgcccga ttaaaagcgg catttgaaaa acgtaatata ccgtttactg
     7081 aaaataatcg ggaccaggcc atgtatcctg ataattgtac catcttagat aatccacttg
     7141 gaacggctca gggtatgtgg tttgaaaaaa acgatacggt gatagcctgc atgcccggtg
     7201 ttccctttga aatgaaaggg ttgatgacca accaggttat acctaaaatt atgaagcggt
     7261 ttaatttccc tgctatcgta aataaatatt ttatgacaag tgggataagt gagagctttt
     7321 tggccaataa attagaaccc ttagaagcta ttttaccaga gcatatcact ttggcttatt
     7381 tacctacacc tgcagtggtt aaactgcgct tgactgctcg cggagaggat gaaaatctgc
     7441 tggaacaaga attatctgct attagcaacc agatgcgggc aatattagga gccgggattt
     7501 atgccgaaga acctataccg cttgaacaac atattggcaa ccaattgagg atgttgaagg
     7561 caactttagc aacggcagaa agtattaccg gcggcaaagt agcgcataaa ataaccctaa
     7621 ttccgggaag ttccgactat tttagaggcg cagttgtagc ctatcaccaa tcagtgaaac
     7681 aacacttact aaaagtttcc acaacaacac tcgaaaactt tacccctgtt agcgaggaaa
     7741 ctgcggtg
//
LOCUS       DAORKW010000118         7721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_281_length_7721_cov_5.198409, whole genome shotgun sequence.
ACCESSION   DAORKW010000118 DAORKW010000000
VERSION     DAORKW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_281_length_7721_cov_5.198409"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="PLB46_06375"
     CDS             complement(<1..1204)
                     /locus_tag="PLB46_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HRF76177.1"
                     /translation="MRKLLLMMIALPLFLVACENKAQTQDNSAIQAEVQQYLDSYNAE
                     YQKLATAAAEGQWVLNTHIVKGDSTASKNAAISDEAYAKFTGSKVNIETAQKYMAEKE
                     KLTAIQVKQLEYILYLAGNNPETAGEIVKKRIDATNAQTEKLFGFAFTLNGKPTTPNA
                     LDEILRSSNDMNQRLAAWNSSKEVGKDLKDGLANLQALRNQSVTPLGYKDFFAYMASE
                     YGMSSEEMLELTHSMINDVWPLYRELHTWARYELADKYKQPVPEYLPAHWLPNRWGQD
                     WTALVNVEGMDIDPELKKQNAEWVVKKGEEFWMSLGFPALPASFYEKSSLYPAPPGAD
                     YSKNNHASAWHMNLDQDVRSLMSVEPNTEWWSTVLHELGHIYYYMSYSNPDVPYVLRT
                     GANRGYHEA"
     gene            1485..2498
                     /gene="recA"
                     /locus_tag="PLB46_06380"
     CDS             1485..2498
                     /gene="recA"
                     /locus_tag="PLB46_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRF76178.1"
                     /translation="MAEATNEKVKALGLAVDSINKLYGKGTIMKLGDSPERGEEIIPT
                     GSIGVDLALGIGGIPKGRIIEIYGPEASGKTTLAIHIIAEAQKKGGICAIIDAEHAFD
                     KVYAEKLGVDVENLLISQPDYGEQGLEIADHLIRSGALDVVVIDSVAALVPKGELEGE
                     MGESKMGLQARLMSQALRKLTGTISKTGCCCIFINQLREKIGVMFGNPETTTGGNALK
                     FYSSIRIDIRRIGQLKDKEELIGNRVKVKIVKNKVAPPFRQAEFDIIYGEGISKIGEI
                     VDLGAESNVIQKSGSWYSYEGTKLGQGRDGVKELLKDNPELAAEIEKKIKDKLNPPVE
                     VKA"
     gene            2533..3915
                     /locus_tag="PLB46_06385"
     CDS             2533..3915
                     /locus_tag="PLB46_06385"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRF76179.1"
                     /translation="MLFNSFEFSCLLLITFFLYYLPAIQKWQVFILIAAGIVFYGFFN
                     PYLLLLLFASASISFVFTYLVIHNTGNKKKLFATIGVAINLGVLLFFKYSGFFADLFL
                     SSDNDMRNFLVSIPLPIGISFYTFHGISLLVDAYRNDESQRWVPTFNYRNSILYTLFF
                     PQLVAGPILRAKYFIPQIVSKQFNQINWVFCFRHLLMGYFLKMVIADNLKEQTAYFSE
                     DYLITSMSSITNIILLIGYACQIFADFAGYSYIARGLAGLFGYTIILNFNYPYIANSF
                     ATFWRRWNIALSQFMHQYLYIPLGGNKKGLWFTYRNIFITMALAGFWHGAALGYLIWG
                     IYHALFLMLERPFRAIKLTTAGGMIKRLVVFFIVVFGWVFFVFPEPERLLLFLQAVQL
                     NWQLPLHYAPIYFTCLYSLPILAFHIYGWRKENGKQPPPVFKQVVFAIMLFMIIVNSG
                     SAGSFIYFQF"
     gene            3917..4750
                     /locus_tag="PLB46_06390"
     CDS             3917..4750
                     /locus_tag="PLB46_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76180.1"
                     /translation="MIKNVVIYTIILFISYYLITLVIPNPVPIPEAPWHKNELKVSLL
                     KTQQSQPPIAIVGTSYSNVLDMQQMDSGCINLSFGGHTALDGILLLRHQKIVPEKLLI
                     EINYGNFDTTGWVNKYIQYPTLFSINPYENRPTVYATGLLVKLLQYDYVAFSRTGQYV
                     DSVAVQNAIAILKQPESQNHNIRDLNDYTLALKKNLVWFEEQGTSIYFYLMPFNPELC
                     DLPYLDKIRKTYLSVFADADYHFILNPDCSPYFTYDGRHLTAEEGEQFTRTLLNSIEI
                     E"
     gene            complement(4756..6021)
                     /locus_tag="PLB46_06395"
     CDS             complement(4756..6021)
                     /locus_tag="PLB46_06395"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRF76181.1"
                     /translation="MKLKSNRTETTNRPTSNYTKKNTPKIQTAGKNPEQKSNPNRKVE
                     RLERAKKYAKQYTEKRSEWDKQKGERDDNYHKRGGRNTSGSRYEKDVPKKKERDLMNK
                     LNKKRDFQSSRFDKKPAFKKVEKKSADFDRNDKFDRTDKVEKSESKYTKRLKLAAPKP
                     VLTKNKFPKKGIPAKLSLKPAALPDTGTRLNKYLANSGVAARRKCDEIIAQGFVKVNN
                     KVVLEMGYKVMPGDKVSYRDKLVKPTNYVYILLNKPKDFLTTVDDEKGRRTVMELIAN
                     ATNERVYPVGRLDRNTTGLLLLTNDGDLAQKLAHPSFGAKKIYEVELDKPVSIQHMNM
                     IREGVELEDGVAYVDGIDFAHATKKNVVGIELHIGKNRIVRRIFEHLGYNVERLDRTL
                     YAGLTKRDLPRGRWRMLTEREIIKLKYLQ"
     gene            complement(5972..6808)
                     /gene="scpB"
                     /locus_tag="PLB46_06400"
     CDS             complement(5972..6808)
                     /gene="scpB"
                     /locus_tag="PLB46_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRF76182.1"
                     /translation="MNDLQKLLEALIFSSEQPISAAELQTILFTYSGEEVSLEAITEQ
                     AELLTMKYESDDFIFEIAKTGGGYQFLSKPYYHKPITTLLQHRAKRKLSVAALECLSI
                     IAYSQPVTKTDLEQIRGVNCDHTIQKLMERDLIKITGRAETAGRPLLYGTTQYFMDYF
                     GINGLEELPRLKEFEQKDSDSSIGSPSDIMENIDEEDAYNQVNLHNERRDDAQNQNNE
                     ETEQQQDEQAIEVLEQPSDENLEQPEDEQISGGGDGDAEEEQDLSETEEQQDGNDESS
                     DK"
     gene            6909..>7721
                     /locus_tag="PLB46_06405"
     CDS             6909..>7721
                     /locus_tag="PLB46_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF76183.1"
                     /translation="MFRNITLLLTFFTLTGSLFAQSINFQPLSLEDAIIESGKTGKPV
                     MFMAYQSTCTHCEKMINEVFPDTMVSNFYNANFINIRIDMLDQAMAKKYIQQFYLSSF
                     PTFIIINGKAETLYQYVGEFKAAEFVKQAKLSLDPANQIPTNRRAFEANPADSTACYN
                     YLLTLSRGRLATQSVASAYFNANNKQLEFTTSNWRILSMSVSNLDSEIFRYMLEHKAD
                     FEKVASTKKVERKFYLTAAYNLQTPATTNDTTNYFRYRNLAAKVGLPIIDSLI"
BASE COUNT         2194 a         1458 c         1454 g         2615 t
ORIGIN      
        1 atgcttcatg gtatccgcga tttgctccgg tgcgtaatac ataaggaaca tcaggattag
       61 aataactcat gtaataataa atatgtccga gttcatgtaa cacggtgctc caccattcag
      121 tattcggttc aacactcatt aatgaacgta catcctggtc taaattcata tgccaagcac
      181 tggcatgatt attttttgaa taatctgctc ccggaggcgc aggatataac gatgattttt
      241 catagaagct ggcaggtaat gcagggaaac cgaggctcat ccaaaattct tcaccttttt
      301 ttaccaccca ttcggcattt tgttttttta attcaggatc aatatccatt ccttctacat
      361 taaccaatgc tgtccaatct tgtccccaac gatttggcaa ccaatgtgca ggtaaatatt
      421 ccggaacagg ttgtttgtat ttatcagcta attcgtaacg cgcccaggta tgtaattcgc
      481 gatataatgg ccacacatca tttatcatac tatgtgttaa ctcaagcatt tcttcgctgc
      541 tcataccata ttcactggcc atgtaagcaa aaaaatcttt gtaaccgaga ggagtaacac
      601 tttggttgcg caatgcctgt aagtttgcca aaccatcttt taaatcttta cccacctctt
      661 tactcgagtt ccaagcggct aaacgctggt tcatatcgtt tgaagagcgt aaaatttcat
      721 ccaatgcatt aggtgttgtt ggtttgccat ttaaagtaaa tgcgaagcca aataattttt
      781 cagtttgtgc attggtagca tcaatacgtt ttttaacaat ttcacctgca gtttccggat
      841 tatttccggc caggtataaa atgtattcca attgttttac ctgaatagcg gtaagttttt
      901 ctttttcagc catatatttt tgggctgttt cgatattcac tttgctaccg gtaaatttgg
      961 cgtaagcctc atcagaaatt gctgcatttt tgctcgcagt tgaatcgcct ttcacaatat
     1021 gggtattgag cacccattgt ccttctgccg cggctgttgc cagcttttgg tattcggcat
     1081 tatagctatc gagatactgc tgtacctcag cctggattgc gctattatcc tgtgtttggg
     1141 ccttgttttc acaagctacc aaaaacagtg gaagggcaat catcattaag agtaattttc
     1201 gcatgtagtt gaattttgta gcgaagttac cgaaatagcc ctgccttggg taatttgtta
     1261 acattcctta tcagttattt aagattataa tcattgtcaa aagcaccaaa tcccacaaat
     1321 tgcgagttaa cactataact ttttcgcgat atgtctacat gatgttgatt ttgcaaaaaa
     1381 ttagtataaa atggcttaat taatatttag cttcgcaaat actttcttcc ccaaaaaagg
     1441 ggttggaggc attttatttc aaaaccttaa aaacaaacaa gattatggca gaagcaacaa
     1501 atgaaaaagt gaaagcctta gggctcgcgg ttgacagtat caacaaattg tatggcaagg
     1561 gcaccattat gaagttgggt gattcacccg aacgcggtga agaaattatt ccaaccggtt
     1621 caattggtgt cgatttagca ttaggcattg gcggtattcc aaaaggacgt attattgaaa
     1681 tttatggccc ggaagcatca ggtaaaacaa ctttggcaat acatattata gctgaagcgc
     1741 agaaaaaagg tggcatttgc gccattattg atgcggaaca tgcttttgat aaggtatatg
     1801 ctgaaaaatt aggggtagat gtagaaaatc tacttatttc acaacccgac tacggtgaac
     1861 agggtttaga aattgccgac catttaattc gctcaggtgc gctagatgtg gttgtaattg
     1921 actctgtagc ggctttggtt cctaaagggg aattagaagg tgaaatgggt gaaagcaaaa
     1981 tgggtttaca agctcgttta atgagtcagg ctttacgtaa attaacaggt accatcagca
     2041 aaacaggctg ttgttgtatt ttcatcaacc agttgcgtga aaaaattggt gtaatgttcg
     2101 gtaaccctga aactactacc ggtggtaatg cattaaaatt ttattcttct atccgcatcg
     2161 atattcgtcg catcggacaa ttaaaagata aagaagaatt aattggtaac cgcgttaaag
     2221 ttaaaatcgt aaaaaataaa gttgcacctc ctttccgtca ggctgaattt gatattattt
     2281 acggagaagg tatttccaaa attggtgaaa ttgtggattt aggcgctgaa agtaatgtga
     2341 ttcaaaaaag cggaagctgg tatagctacg aaggcacaaa actcggtcag ggacgcgatg
     2401 gcgttaaaga attattgaaa gataatcctg aactcgctgc agaaattgag aaaaaaatta
     2461 aagataaact caatccacct gttgaagtaa aagcataatt gaaaataggg cagattaatt
     2521 aaacagaact atatgttgtt taattcattc gaatttagct gcctgctttt aattactttc
     2581 tttttatatt atttacctgc catccaaaaa tggcaggttt ttattttaat agcagccggt
     2641 attgtttttt acggattttt taatccttac ctcttactgc tactatttgc aagtgcaagt
     2701 atttcgtttg tatttactta cctggtaatt cataatacag ggaataagaa aaaattattc
     2761 gccaccatag gtgttgctat taacttaggt gttctgctat tttttaaata cagcggtttt
     2821 tttgccgacc tattcttaag cagtgataac gacatgcgca attttttagt gagtatccca
     2881 ctgccaatag gtatatcgtt ttatactttt catggcatca gtttgttggt tgatgcttat
     2941 cgcaacgatg aaagtcagcg ctgggtacct acattcaatt atcgaaattc cattttatat
     3001 accttgtttt ttcctcagct agtagcaggg ccaattttac gcgctaaata ttttattcct
     3061 caaattgtca gcaaacaatt taatcagata aactgggtat tttgtttcag gcatttgctg
     3121 atgggctatt ttttaaaaat ggtaattgcc gataatttaa aagaacaaac agcttatttc
     3181 tcggaagatt atttgataac atccatgtca tctattacta atattatttt attgattggt
     3241 tatgcatgtc agatttttgc cgattttgcc ggctactctt atattgccag agggcttgcc
     3301 ggtttgtttg gatataccat cattttaaat ttcaattatc cttatatcgc taatagtttt
     3361 gccacatttt ggaggcgatg gaatattgcg ctatcgcaat ttatgcatca gtatttatat
     3421 attccattag gaggcaataa aaaaggatta tggtttactt atcgcaatat ttttattacc
     3481 atggcgttag cgggattttg gcatggcgca gcgttagggt atttgatatg gggaatttac
     3541 catgccctat ttttaatgtt agaacgacca tttagggcaa ttaaattaac aactgcagga
     3601 ggtatgataa aacgtttggt tgtgtttttt atcgtagttt ttggatgggt attttttgtt
     3661 tttcctgaac ccgagcgttt actattattt ttacaagcgg tgcaactaaa ttggcaacta
     3721 ccattacatt atgcacccat ttattttacg tgtttataca gtttacccat tctggctttt
     3781 catatatatg gttggaggaa agaaaatggt aaacaacctc ctcctgtgtt taagcaggta
     3841 gtttttgcca ttatgttgtt tatgataatt gttaattcag gtagtgcagg aagttttatt
     3901 tattttcaat tttaacatga ttaaaaacgt tgtaatatat actatcattt tatttatcag
     3961 ctattacctg attacactgg taatacctaa ccctgtacct attccagaag cgccatggca
     4021 taaaaatgaa ttaaaggtaa gtttattgaa gacacaacaa tcgcaaccac ccatagccat
     4081 agttggcact tcgtatagta atgtgctgga tatgcaacag atggattcag gttgtatcaa
     4141 tttgtcgttt ggcgggcata cggcattaga tggtatttta ctattgcgtc accaaaaaat
     4201 tgttcctgaa aaattattaa tagaaattaa ttatggcaat tttgacacca ccggttgggt
     4261 aaataaatat attcagtatc caacactatt ttccatcaat ccgtatgaaa acagaccaac
     4321 ggtttatgct acaggattat tagtgaagtt gttacaatat gattacgttg catttagccg
     4381 cacaggtcag tatgtggaca gtgttgcagt gcaaaatgca attgccatat taaaacaacc
     4441 ggaatcgcaa aatcataata tccgcgattt aaatgattat actttagcac tcaaaaaaaa
     4501 cttagtttgg tttgaagagc aaggcacttc catttatttt tatctcatgc catttaatcc
     4561 ggaattatgc gatttaccgt atttagataa aataagaaaa acctatttat cggtatttgc
     4621 ggatgctgac tatcacttca tattaaatcc ggactgttca ccttatttca catacgatgg
     4681 caggcattta actgctgagg aaggagaaca atttacaagg acattattaa attcaatcga
     4741 aatcgaataa actatttatt gcaaatactt cagcttaata atttctcttt cagtaagcat
     4801 tctccaccgg ccacgaggta aatcgcgttt agttaatcca gcataaagtg tgcggtctaa
     4861 tcgttcaaca ttataaccta aatgttcaaa tatgcgtcga acaattcgat ttttaccaat
     4921 atgtaattct atacctacaa catttttttt ggtagcatgt gcaaaatcaa ttccatcaac
     4981 ataagcaaca ccatcttcta attcaacacc ttcgcgaatc atattcatgt gttgaataga
     5041 aacaggttta tcgagttcaa cctcatatat ttttttcgca ccaaaacttg ggtgagcgag
     5101 tttttgtgct aggtcaccgt cgttagttaa taataataat cctgtagtat ttctatccaa
     5161 tctgccaaca gggtacacgc gttcgttagt agcgttggca attaattcca ttactgttct
     5221 tctacccttt tcatcatcaa ccgtagttaa aaaatctttg ggtttattta agaggatgta
     5281 cacataattg gtaggtttta ccaatttatc gcggtagcta actttatcgc cgggcattac
     5341 tttgtagccc atttcgagca ccactttatt gtttactttg acaaaaccct gtgcaataat
     5401 ttcatcacat tttcttcttg ccgcaacacc ggaattagcc aggtatttat ttaatcgtgt
     5461 acctgtatca ggtaatgctg ccggttttaa acttaatttt gcaggtatac cttttttagg
     5521 aaatttattt tttgtcagca ccggttttgg cgcagctaat tttaatcgtt tagtgtattt
     5581 actttccgat ttttctactt tatcggtgcg gtcaaattta tcgttgcggt caaaatctgc
     5641 cgattttttt tctacttttt taaatgcagg ttttttgtcg aaacggctgg attggaaatc
     5701 gcgtttttta ttgagtttat tcattaaatc gcgttccttc tttttgggaa catctttttc
     5761 atatctgctg ccggaagtat ttcttcctcc gcgtttatga taattatcgt cgcgttcacc
     5821 tttttgttta tcccattcgc tgcgtttttc ggtatactgt ttcgcatact ttttcgcacg
     5881 ctcgaggcgc tctaccttgc ggttaggatt acttttctgc tcgggatttt tccctgcagt
     5941 ctgaattttt ggcgtattct ttttagtgta gttacttgtc ggacgattcg tcgtttccgt
     6001 cctgttgctc ttcagtttca ctgaggtcct gctcttcttc ggcatctccg tctcctcccc
     6061 ctgaaatctg ttcgtcttcc ggttgctcga ggttttcgtc ggacggctgt tcgaggactt
     6121 cgatggcctg ctcgtcctgt tgttgttcgg tttcttcatt gttttggttt tgggcatcat
     6181 cacgacgctc gttatgtaaa ttcacctgat tataagcatc ctcctcatca atgttttcca
     6241 tgatgtcgga tgggctgcct atacttgaat cagaatcttt ttgctcaaat tctttcaaac
     6301 gtggcaattc ttcgagacca ttaatcccga aatagtccat aaaatattgt gtagtgccat
     6361 acaacagcgg tctacctgct gtttccgcac gtcctgtaat tttaatcagg tcgcgttcca
     6421 tcagtttttg gatggtatgg tcacagttta caccacgtat ttgctcgagg tcggttttgg
     6481 taaccggctg agagtaggca ataatggaga ggcattcaag ggctgccacc gacaatttgc
     6541 gttttgcacg atgttgtaac agggtggtta tcggcttatg gtaataaggt tttgataaaa
     6601 actggtatcc accgccggtt ttagcgattt cgaaaataaa atcgtcgctt tcgtatttca
     6661 tggtcagcag ctcagcctgc tcagtaatag cttccaggct cacttcctca ccggagtagg
     6721 tgaataatat cgtttgcagc tcagcagccg aaatgggttg ctcgctactg aatatcaagg
     6781 cttctaagag tttctgaagg tcgttcattg cggtgcaaat ctaaggaatt gttggggcac
     6841 acagcgcttt ttggtgagat ttttgtaagt aaatcgacaa ttcacctaca agcgataact
     6901 ttgaagctat gtttcgaaat attaccttac tccttacatt tttcacgcta acaggcagcc
     6961 tatttgccca aagcatcaac tttcagcctt tgagcctgga agacgccatt attgaaagcg
     7021 ggaaaaccgg caaacctgtc atgtttatgg cctaccagag cacttgcact cactgtgaaa
     7081 aaatgattaa tgaagtattt cccgatacca tggtaagtaa tttttacaat gccaatttca
     7141 tcaatatccg tattgacatg ttggatcagg ccatggcaaa aaaatatatt caacaatttt
     7201 atctttcttc ttttcctaca tttatcatca taaatggcaa agcagaaaca ttgtatcaat
     7261 atgtcggtga atttaaagca gctgagtttg taaagcaggc aaagttgtcg ttagaccctg
     7321 ccaatcaaat tccaaccaac agaagggcat tcgaagccaa cccagccgat tccactgcgt
     7381 gttataatta tctattaaca ttatctcgtg gccgcttagc tactcaaagt gttgcttctg
     7441 cctattttaa tgctaacaac aaacaattag aattcaccac ttcaaactgg cgcattttaa
     7501 gtatgagcgt aagtaattta gattctgaaa ttttcagata catgttggag cacaaagcag
     7561 attttgaaaa ggtagcttcc acaaaaaaag ttgagcgcaa attttattta acggctgctt
     7621 acaatttgca aacgcctgca acaacaaacg atactactaa ttattttcga tatagaaatt
     7681 tagctgctaa agtaggcttg ccgataattg attcattaat a
//
LOCUS       DAORKW010000119         7685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_284_length_7685_cov_6.235125, whole genome shotgun sequence.
ACCESSION   DAORKW010000119 DAORKW010000000
VERSION     DAORKW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_284_length_7685_cov_6.235125"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3243)
                     /locus_tag="PLB46_06410"
     CDS             complement(<1..3243)
                     /locus_tag="PLB46_06410"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRF76184.1"
                     /translation="MASAKKQKAGLQFDRYFTKEGVSPFDQYEYELRSSVIRNTTGDA
                     VFEMYNVEVPKEWSQVATDILAQKYFRKAGVPQADGSLGSETSIKQVAHRLADCWKQW
                     GEDYGYFSSKKDANIFYEELVYMIVGQLAAPNSPQWFNTGLYSTYGIKGKPQGHYYVD
                     PVTEELKKSTSAYERPQPHACFILSVDDDLVNQGGIMDLWTREARIFKYGSGVGTNYS
                     KIRGEGEKLSGGGTSSGLMSFLKIGDAAAGAIKSGGTTRRAAKMVCLDLDHPEIETFI
                     DWKVKEEDKVAALIAAGYSSDYEGEAYRTVSGQNSNNSVRVSNKFFKALEEEKEWELI
                     GRSNGKVMKTVDAKKLWEKIAYAAWRCADPGIQYDTTINEWHTCPKSGRINASNPCSE
                     YMFLDNTACNLASINLRKFYDDDKLVFNVEAFEHACRIWTVVLEVSVLMAQFPSKEVA
                     QLSYEFRTLGLGFANLGSVLMVSGIPYDSDKARAIAGSISAIMTGISYKTSAEMAGVL
                     GTFKGYDLNKEDMMRVMRNHRYAAYNASENYENLEIKPMGIDQALCPEYLLKAACNAW
                     DGALQLGEQFGYRNAQATVIAPTGTIGLLMDCDTTGVEPDFALVKFKKLSGGGYFKII
                     NQSIPLALKNLGYKDESIQAIVDYAKGTGTLKGAPHINFESLKLKGFTHEEVEHVDSI
                     MAAAFDISFAFNVFTLGEATMERLGYSAEHYTEPGFNLLRALGFSREEIEAANNHICG
                     TMTIEGAPQLLEKHYSVFDCANKCGKIGERYIHQYGHVRMMGAVQPFISGAISKTINL
                     PNEATVEDIEGCYMMSWQLGLKANALYRDGSKLSQPLSTKSDAKEEKEAPKHEWTSEE
                     VLEAARSIIMQSTDTIFKRQLSRVVERKKLPAKRDGFTQKSKIGGQTIFVRTGEYEDG
                     TLGEIFIDMHKEGASYRSLLNCFAIAVSIGLQYGVPLEEFADKFTFTRFEPSGPVDHA
                     NIKFSTSIVDYVFRLLGFEYLNRTDLVHVAPEDLSTLEKSTQDISTEATGNYPVTEPI
                     AESKEKKAQATRKATAEKYEIVNATDAANKSLMGDAPPCPKCGHTTIRNGT"
     gene            3591..3842
                     /locus_tag="PLB46_06415"
     CDS             3591..3842
                     /locus_tag="PLB46_06415"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HRF76185.1"
                     /translation="MKTKLTLSIDKNVIKKARSISRNQKRSVSALFEEYIERLYEIND
                     TSEKKSPTIVLSKGIIKWKYPEQITYKELIKNKEDKPAR"
     gene            3839..4258
                     /locus_tag="PLB46_06420"
     CDS             3839..4258
                     /locus_tag="PLB46_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRF76186.1"
                     /translation="MKTVFIDTDILLDFYFGRKPFGDYAEQVLVLCENKKLEAFISTQ
                     SIITCYYFLRKEIGKVKAVEHLQQLLHIVEIIEINKQDILMAFNSNFTELEEALLHNA
                     ATKTEFTDAIITRNAKDFKNAVVPVVNANDFLKKFYL"
     gene            4424..5146
                     /locus_tag="PLB46_06425"
     CDS             4424..5146
                     /locus_tag="PLB46_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRF76187.1"
                     /translation="MKNVVLHTANTRGHANHGWLDSYHTFSFAQYYNPERVNFGVLRV
                     LNDDTVAAGMGFGKHPHDNMEIISIPLEGYLEHQDSMGNKTVIRSGDIQVMSAGTGVT
                     HSEYNKNSDKPVKFLQIWVFPNKKNVAPRYDQITLNEADSKNKLQQILSPNPDDAGVW
                     IHQNAWFHLGKFDNGIVTDYTLNDKNNGVYFFVLNGSVKIDEQLLNRRDGYGVWDITS
                     LKITSNEPNTKILVMEVPMKLN"
     gene            5160..5711
                     /locus_tag="PLB46_06430"
     CDS             5160..5711
                     /locus_tag="PLB46_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRF76188.1"
                     /translation="MSTATTTTRNWVVDPTHSTIGFSVKHLMMTTVRGEFEKYTIDVT
                     TQGEDFMTAVIAFAAETASVNTRNEGRDTHLRSDDFFNAEAYPAITFKPTKFENVDSD
                     GSYELYGDLTIRDVTKNIKLDVEFGGVMKDPWGNAKAGFTINGKINRTDFKLNWNAAL
                     ETGGVLVSEDVRINCEIQLVEVK"
     gene            complement(5851..6828)
                     /locus_tag="PLB46_06435"
     CDS             complement(5851..6828)
                     /locus_tag="PLB46_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76189.1"
                     /translation="MKIKYWRLIIWNRGLVILLLSSFVWSCKPDEQIIIDDDPCFFPD
                     IDFGINGPIADYAAIQYLKPCFNPSNENEIVYIEEQPTSGISRLCVLNIVDLTISYIQ
                     DNVSGSPKWSKTDWIIFNKDEDIWKIKPDGDSLTLLFSNGTNYSPEVNPRGDKIIFKY
                     QLDNYIAIKISDLNGNVIDSLEDQALYCPSWSPDGTKISMRRAGEPSSFGYYDTTLTT
                     FSPIITNTSGSPFDMISDTRWFPDSEHIFWFGSGFYSITNITTMETEMFRDYCPKNSL
                     LYPNFSSALDIIIWEKNTIKLLENNSGYIISTSIVMTDLDGANEVVLLE"
     gene            complement(7176..>7685)
                     /locus_tag="PLB46_06440"
     CDS             complement(7176..>7685)
                     /locus_tag="PLB46_06440"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF76190.1"
                     /translation="IYPGAIELCNYLDDDCDGITDDNLTYLLSYQDNDGDDFGNPLID
                     SLSCELPLGYVVNNTDCDDTNAEIYPGANETLNGLDDDCDQIADEGLAVNDIVKNTIH
                     IFPNPVNTVLFIQSNETQNITIVNQLGEEILHTNLFIGLNTISVADFASGMYWVKAEN
                     GEIVVWVKE"
BASE COUNT         2469 a         1390 c         1411 g         2415 t
ORIGIN      
        1 agtaccgttg cgaatagtgg tgtgaccaca tttcggacaa ggtggagcat cgcccattaa
       61 cgatttgtta gctgcatctg tagcattaac tatttcgtat ttttcggcag tagcttttct
      121 tgttgcttgt gcttttttct cttttgattc tgcaatcggt tcagttaccg gatagttgcc
      181 ggtagcttcc gtcgaaatat cctgagtcga tttttctaaa gtacttaaat cttccggtgc
      241 aacatgcact aagtcggtac gatttaaata ttcgaatcct aataaacgga atacataatc
      301 tactatcgaa gtactgaatt taatattggc gtgatcaacc ggtccgcttg gttcgaaacg
      361 ggtgaaagta aatttatcag caaattcttc cagtggcaca ccgtattgta aaccgataga
      421 tactgcaata gcaaaacagt ttaacaatga acgataggaa gcaccttctt tgtgcatatc
      481 gataaagatt tcacctaatg tgccatcttc atattcacct gtgcgcacaa atatggtttg
      541 accaccaatt ttagattttt gtgtaaagcc atcgcgtttc gcaggtaatt ttttgcgttc
      601 aactacgcgt gacaactgac gtttgaaaat agtatcagtt gactgcataa taatgctgcg
      661 tgctgcttcg agtacttctt ctgaagtcca ttcgtgtttt ggtgcttctt tttcctcttt
      721 tgcatcgctt ttggtagaaa gtggttgtga taatttagag ccgtcgcgat ataaagcgtt
      781 tgcttttaat cccaactgcc agctcatcat ataacagcct tcaatatctt caacagttgc
      841 ctcgttaggg aggttgatgg ttttagaaat agcccctgaa ataaacggtt gaacggcacc
      901 catcatacgc acgtgaccgt attggtggat ataacgttca ccaatttttc cgcatttatt
      961 tgcgcaatca aatactgaat aatgtttttc taataattgt ggtgcacctt caattgtcat
     1021 ggtaccacaa atgtgattat tagcagcttc aatttcttct cttgagaaac ctaatgcgcg
     1081 taataaatta aatccgggtt cagtataatg ttcggcgctg taaccaaggc gttccatggt
     1141 tgcttcacct aaagtgaata cgttaaatgc aaagctgata tcaaatgcag cagccattat
     1201 tgaatccaca tgttccactt cttcatgcgt aaaacctttc agttttaatg attcgaaatt
     1261 gatatgtggt gcacctttta atgtaccggt tccttttgca taatcaacaa ttgcctgaat
     1321 actttcgtct ttatatccaa gatttttgag tgcaagcgga atactttggt taataatttt
     1381 gaaataacca ccacctgata attttttgaa tttcactaaa gcaaaatcag gttcaacacc
     1441 ggtagtatcg caatccatta ataaaccaat tgttcctgtt ggagcaatta ctgtggcttg
     1501 cgcgttgcgg taaccaaatt gttcacctaa ttgcaatgcg ccatcccatg cgttacaagc
     1561 cgctttgagt aagtattcag gacataatgc ctgatcgata cccattggtt tgatttcgag
     1621 gttttcgtaa ttttctgatg cattatatgc tgcataacgg tggttacgca taacacgcat
     1681 catatcttct ttatttaaat cgtaaccttt aaatgttcct aaaacgcccg ccatttcagc
     1741 cgatgtttta taggaaatac cggtcatgat agcagaaata gaacctgcaa ttgcacgagc
     1801 tttatcgcta tcataaggaa tacccgatac cattaataca gaaccgaggt tagcaaatcc
     1861 taaaccgagt gtacggaatt cgtaagataa ttgcgctact tcttttgaag ggaattgcgc
     1921 cattaatact gaaacttcca atactacagt ccagatgcga caagcatgtt caaatgcttc
     1981 tacattaaat acgagtttat cgtcgtcgta aaacttgcgt aagttgatag atgcaaggtt
     2041 acaagcggta ttgtcgagga acatatattc gctgcatggg ttagatgcat tgatacgtcc
     2101 tgatttagga catgtatgcc actcgttgat agtagtatca tattgaatac ctgggtcagc
     2161 acaacgccac gcagcgtaag caattttttc ccacaatttt ttcgcatcaa cggttttcat
     2221 cactttaccg ttagagcgac caatcaattc ccattctttt tcttcttcaa gtgctttgaa
     2281 gaatttatta gaaacacgaa ccgagttatt agagttttgt cctgaaactg tgcggtaagc
     2341 ttcaccttca taatcagatg aataaccagc agcaattaag gcagcaactt tatcttcttc
     2401 ttttactttc cagtcaataa acgtttcaat ttccgggtga tcgaggtcta aacacaccat
     2461 tttggccgca cgacgtgttg ttccacctga tttaattgca ccggcagcag catcaccaat
     2521 ttttaagaag ctcattaagc cacttgatgt tccgccacca gataattttt caccttcacc
     2581 acgaattttc gaataattgg taccaacacc tgagccgtat ttaaagatac gtgcttcgcg
     2641 tgtccacaaa tccataatac caccctgatt tactaaatcg tcgtccacac ttaaaataaa
     2701 gcaagcatgt ggctgagggc gctcgtaagc agaggtagat tttttaagct cttctgttac
     2761 cgggtcaaca taataatgtc cctgtggctt tcctttaatg ccataggtag aatataaacc
     2821 ggtattgaac cattgtggtg agttaggagc agctaactga ccaactatca tatatactaa
     2881 ctcttcataa aagatattag catctttttt tgatgagaaa taaccatagt cttcacccca
     2941 ttgcttccag caatcagcta aacggtgtgc aacctgttta atagaggttt cgcttcctaa
     3001 tgaaccatca gcttgtggaa caccagcctt gcggaaatac ttttgcgcaa gaatatcggt
     3061 agccacttga ctccattctt taggaacttc aacattatac atttcaaata ctgcgtcacc
     3121 ggttgtattc cggataacgg atgaacggag ctcgtattcg tactggtcga aggggctcac
     3181 tccttctttt gtaaagtagc ggtcaaattg taaccctgct ttttgttttt tagcgctggc
     3241 cataaattgt gataagattc gtttcggttc gtaattggtt attgacagaa aaacctccca
     3301 cttttcggcg agaggtttta cgaaagtatg acataaaagt gtttacaaac aagtcacact
     3361 tttccacctc catgttcata aacgaccttt tgtatgattt acagtgcatc gggtttgtac
     3421 acatatattt ttatataata tgattgatga aacgtactgt gctgttatgt tgaaataaac
     3481 atataaaatg gggaaaacat ttcataaaaa aatttggatg ccgaaaaaag agatgtttaa
     3541 taggtataaa atgaataaaa caatacgtat ctttattttt catacgtata atgaaaacaa
     3601 aactcacttt aagcatcgat aaaaacgtga ttaaaaaagc acgttccatt agtcgtaatc
     3661 aaaaacgttc ggtttctgct ttgtttgaag aatacattga acgcttgtat gaaataaatg
     3721 atacatcaga aaaaaaatcg ccaactattg tattatcgaa aggtatcatt aaatggaaat
     3781 atcctgaaca aattacttac aaagaattaa ttaaaaataa agaagataaa cccgctcgtt
     3841 gaaaaccgtt tttattgata cggatatttt gctcgatttt tatttcggga gaaaaccttt
     3901 tggcgattat gctgaacagg tattggtatt atgtgaaaat aaaaaactag aagcatttat
     3961 cagcacacaa agtattatta cctgttatta ttttttgcgt aaagaaatag gtaaagtaaa
     4021 agccgttgaa catttacaac agttattaca tattgtagaa attattgaaa taaataaaca
     4081 agatatttta atggcattta attctaactt cactgaattg gaagaagcac tattacataa
     4141 tgctgctaca aaaacggaat tcaccgatgc cattattacc cgcaatgcca aggattttaa
     4201 aaatgccgtg gtgccggtgg tgaatgcgaa tgattttttg aaaaaatttt atttgtaatg
     4261 tttaaatgtg ttggggcgga taatcatccg cccccacggg gcggggcgaa taaaaatgcg
     4321 tgttttttgg ttttaatgaa ctttgtgggc aatttatatg tatatacata caaattgctg
     4381 aaatttgcga tattcgcaca gcgtaatcaa aaaaggaaac gttatgaaaa atgttgtatt
     4441 acatacggca aacactagag ggcacgctaa ccacggttgg ctagactctt atcatacgtt
     4501 cagttttgct caatattata atcctgaacg ggtgaatttt ggtgtgttaa gggtacttaa
     4561 cgacgatact gttgcggcgg gaatgggctt tggcaaacat ccgcacgaca atatggaaat
     4621 tatttccatt ccgctggaag gctatttgga acatcaggat agtatgggta ataaaacggt
     4681 aatccgttcg ggtgatattc aggtgatgag tgccggaacc ggcgttacgc atagcgaata
     4741 caataaaaac agcgacaaac ccgtaaaatt tttacagata tgggtgtttc cgaacaaaaa
     4801 aaatgtggcg ccgcgttatg atcagattac attaaatgag gctgacagta aaaataaact
     4861 acaacaaata ttatcaccta atccggatga tgccggtgta tggatacatc aaaatgcgtg
     4921 gttccatttg ggcaaatttg ataatggtat tgttacagat tataccttaa atgataaaaa
     4981 caacggcgtt tatttttttg tattaaacgg ctccgtaaaa attgatgagc aattattaaa
     5041 tagacgcgac ggatatggtg tatgggatat tacatcttta aaaataactt caaatgaacc
     5101 aaacacaaag atattggtta tggaagttcc aatgaaatta aattaaacaa atacataaaa
     5161 tgagtacagc aactacaaca acaagaaact gggtggtaga tccaacccat tcaactatcg
     5221 gatttagtgt aaaacattta atgatgacaa ccgttcgtgg tgaattcgaa aaatacacta
     5281 tcgacgtaac tacacaaggt gaagatttta tgactgcagt aattgcattt gcagcagaaa
     5341 ctgcaagcgt aaacacccgc aacgaaggtc gcgacaccca tttacgcagc gatgattttt
     5401 ttaatgcaga ggcatatcct gcaattactt tcaaaccgac aaaatttgaa aatgtagaca
     5461 gtgatggcag ctacgaatta tacggcgatt taaccatccg cgacgtaaca aaaaacatta
     5521 aactggatgt tgaatttggt ggtgtaatga aagacccttg gggaaatgct aaagccggat
     5581 ttaccattaa cggaaaaatt aaccgtaccg attttaaatt aaactggaat gccgctttgg
     5641 aaacaggtgg tgtattggtg agtgaagatg ttagaattaa ttgtgagata cagttggtag
     5701 aggtgaagtg aggctgtggc gtcgatttaa aagattgata atatttgaac ccgttgatta
     5761 ctcaacgggt tttttatttt ttgattgaac aagtttgagc aaattttaat acatagacaa
     5821 aatatcatct tgatttctag tgtaataatt ttattccaac aaaactactt cgttagcgcc
     5881 atctaaatca gtcatgacaa ttgaagtgga aataatgtag cctgaattat tttctaataa
     5941 tttaatggtg tttttttccc aaatgattat atctaatgcc gaagagaaat ttggatataa
     6001 taaggaattt ttagggcaat aatcacgaaa catttcggtc tccatagttg tgatgttagt
     6061 gatactgtaa aatccggagc caaaccaaaa aatgtgttct gaatcaggaa accatcttgt
     6121 atcactaatc atgtcaaaag gagaacctga agtatttgtg atgataggac tgaatgtagt
     6181 taaagtagta tcgtagtaac caaaggaaga tggctcacca gctcttctca ttgaaatttt
     6241 tgtcccatca ggtgaccaac ttggacaata tagagcttgg tcttctaaac tatcaattac
     6301 atttccattt agatcagaga ttttaattgc gatataatta tctaattgat atttaaaaat
     6361 aattttatca ccacgtggat taacttccgg cgaataatta gtcccgtttg aaaaaagtaa
     6421 tgttaaacta tcaccatcag gtttgatttt ccatatatct tcatctttat taaatattat
     6481 ccagtctgtt ttactccatt tgggtgatcc cgatacattg tcttgtatat atgaaattgt
     6541 aaggtcaaca atgtttaata cacaaaggcg tgaaattccc gaggttggtt gttcttcgat
     6601 atatacaatt tcattttcat tagatggatt aaaacaaggt tttaaatact gaattgctgc
     6661 ataatcggca attgggccat ttataccaaa atcaatatca ggaaaaaaac acggatcatc
     6721 atcaattatg atttgttcat caggtttgca tgaccaaacg aatgacgaga ggagtagaat
     6781 gactaaacca cgattccaaa taattaatct ccaatattta attttcaagt taaagctatt
     6841 tacgataatg gtataaaaat atataaaaaa agatactatg agtatattaa atgaataaat
     6901 ttaaaacggc ctacttgctt gtaaacgaca atcatcgctt cgtaggtgtc ctcaattata
     6961 aattgtaagg gcggatgcaa ttcgccccta caatttataa tcgaggacag attgtagaaa
     7021 aaatgaaatt aaaaagcgtt atgccttcaa ctaaaatgat atttttaaaa taaattgagc
     7081 aaatggtcga ggtttgtagg ggcgaattgc atccgcctat acaagcctcg aatatttgcg
     7141 atggcatagg agaaatgcaa ttcaaatatc gatgcttact ccttcaccca aaccactatc
     7201 tctccatttt ccgctttcac ccaatacata ccactcgcaa aatcggctac cgaaatcgta
     7261 tttaatccga taaataaatt tgtgtgtaat atttcttcac ccaattgatt tacaatggta
     7321 atattttgtg tttcatttga ttgaataaat aaaacagtat ttacaggatt agggaaaatg
     7381 tgaatggtgt tttttacaat atcattcaca gctaaacctt catcagcaat ttggtcgcaa
     7441 tcgtcgtcga ggccgtttaa tgtttcatta gctcctgggt aaatttcagc attcgtgtca
     7501 tcacaatcag tattatttac aacataacca agtggtaatt cacatgaaag tgaatctatt
     7561 aatggattac caaaatcatc accgtcatta tcttgatagg ataaaaggta agttaaatta
     7621 tcatcggtta ttccatcaca atcatcatct aaataattac agagttcaat agctccggga
     7681 tatat
//
LOCUS       DAORKW010000120         7628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_289_length_7628_cov_5.652714, whole genome shotgun sequence.
ACCESSION   DAORKW010000120 DAORKW010000000
VERSION     DAORKW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_289_length_7628_cov_5.652714"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2290
                     /gene="carB"
                     /locus_tag="PLB46_06445"
     CDS             <1..2290
                     /gene="carB"
                     /locus_tag="PLB46_06445"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRF76191.1"
                     /translation="TGGGFVQHKDELDAALMRGLTASPTHEVLVEKAVLGWKEFELEL
                     LRDAADNVVIICVVENLDPMGVHTGDSITVAPAMTLSDTCYQEMRNLSIKMMRAMGNF
                     AGGCNVQFAQDPYTEELIAIEINPRVSRSSALASKATGYPIAKIAAKLAIGYRLDELS
                     NQITKSTSAYFEPALDYVVVKMPRWNFDKFPGADDTLGLQMKSVGEVMSIGRSFQEAL
                     QKACQSLENNKIGLGGEKGFYKSSQEIIDSLAKPHWDRIFNIKKAMDLGVPVRTIHNQ
                     SLIDPWFLMQIQELVKIEKEVRRYQLNNIPHKLLLTLKQKGYSDLQIANNLGNCTEEE
                     VYNHRRSLGIKRTYKLVDTCAAEFEAKTPYYYSTFEDENESVSSDKKKVIVLGSGPNR
                     IGQGIEFDYCCVHGVLAIKESGYEAIMVNCNPETVSTDFDIADKLYFEPVFWEHLWEL
                     IEHEKPEGVIVQLGGQTALKLAKQLHEKGIKIIGTSYDNMDIAEDRGRFSDLLKELGI
                     PYPEYGAATTVEEATEVAHRIGYPVLVRPSYVLGGQRMRIVINDEELERSVIGILKHF
                     PDNRILIDHFLDRAEEAEIDAICDGTDVHVMGIMEHIEPAGIHSGDSSAVLPPFHLSA
                     HVIAQMEDYTTKIAHALNIRGLINIQFAIKNEKVYVIEANPRASRTTPFIAKAYQIPY
                     LNVATKVMLGVKKLSDFTYDKKLTGYAIKEPVFSFEKFPNVNKELGPEMKSTGEAIRF
                     IADLTDPAFREMYSKKSMYLSR"
     gene            2590..2778
                     /locus_tag="PLB46_06450"
     CDS             2590..2778
                     /locus_tag="PLB46_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76192.1"
                     /translation="MSDIFWIILILGLYWYIIRPMMQGILEQNKSAPSGRQQSPSTPK
                     KQQSEKRDGEYVDYEEIK"
     gene            complement(2779..3483)
                     /locus_tag="PLB46_06455"
     CDS             complement(2779..3483)
                     /locus_tag="PLB46_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HRF76193.1"
                     /translation="MQTFVIHVKGVDHRLKHMQQELVKANLSAEFILEGDKHELNPSL
                     LANYFTGKMAVVSNASSCAYKHIKAYEEMVRLELPEAIIFEDDIYLDKHFSEKLQQCK
                     SEIKTRQLENYIISLEETSLRYVKGSKRKKGTLLYPNSRGRMAGAYLVDYKAASNMLA
                     DLAQNKTDQAIDWYHNHCINKQIINMFWIHPTIAIQGSISGRLDSTISNRRTNYFRIS
                     SFYLQRWYKKILWGLR"
     gene            complement(3717..4292)
                     /locus_tag="PLB46_06460"
     CDS             complement(3717..4292)
                     /locus_tag="PLB46_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76194.1"
                     /translation="MKNKLLLVVATIIMAASTNKTHAQEFMAGNINVNVGFGIGGYLS
                     YVSFGDFKTSPLLTVSLDYAYMDDVGPGTIGIGGLLGYKSSSYEYNYFGYTDKGSWKD
                     FVVGGRGSYHVYLDIPKVDIYGVVNLGVVVETYDYSSDSPLTVGNNSTSVGLYGGLCA
                     GAKYFFTDNLAAYAELGYDVAWIKLGVTVRM"
     gene            4544..5632
                     /locus_tag="PLB46_06465"
     CDS             4544..5632
                     /locus_tag="PLB46_06465"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRF76195.1"
                     /translation="MSTKPRYANANNEEKVFIDTLRKKVNQYFKVQHLPKHAGNKLLL
                     RGLILLVLLLIIRTLAVISATQIGLFYFSYVLTGIIALPIILNIGHDAVHNNFSGNPF
                     INKLGSGVFYLFGTSTYFWKLRHIYAHHAFTNVPGWDLDITQSNIIRLHDGQKRKPFH
                     RFQYLYMPFLFSVYTLNWFFYRDIKDIFCHKFGNKTIDKHASTQIVLLIVSKLFFLFY
                     MLVVPMVFQHVELHEALFAFLLFNLSAGLTVTLALVSTHVGESQEFVAITDNTLPYSW
                     EMHQLQTTADFATRNKFITWFFGGFNHHVAHHIFPGISHIHYAAVTPIIKETTAAFGI
                     TYHESPTLLTAIREHFTLLKIKGLKEEF"
     gene            5649..6242
                     /locus_tag="PLB46_06470"
     CDS             5649..6242
                     /locus_tag="PLB46_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76196.1"
                     /translation="MRIATIADRATVVNIIVAMFKHNPTIQFMVKNDNKANKRITAIA
                     EYAFDFAVARDGVYISDNNKGVAICYQYNLKAITLKDIGLMLKMVFKAIHISKLFQIF
                     LHDNFIKSQRPVDGNYYYFWFFGVLAEEQPKISARDLTIALFEKAKIKRIPIYAETTL
                     EKNKKVYERFGFKVYKTWINQTNGIQVWFMCRDFRMA"
     gene            complement(6270..6563)
                     /locus_tag="PLB46_06475"
     CDS             complement(6270..6563)
                     /locus_tag="PLB46_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76197.1"
                     /translation="MQEIIDKVKQSSGITDEQAKNSINTVAEYLKSKMPKSLHSQIDN
                     MLKGEKFSDSLKETFMDAAVEAKEKSQEVLKDVVEKTEEAAKNIKEKVSNLFK"
     gene            6776..7561
                     /locus_tag="PLB46_06480"
     CDS             6776..7561
                     /locus_tag="PLB46_06480"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HRF76198.1"
                     /translation="MRKRNVWPVIIAAIALIGTITMTSCNKDNQDPTGTAHVNMYLTD
                     APADYDAVYIDVQSIEIISDAGTTTHTLLHPGVYDLLKFNSGVDTLMISEDLSPRTVS
                     QIRLILGENNSVVVDGVSYPLSTPSAQTSGLKLNVHYTFEAGISYNLWMDFDASQSIV
                     EQGNGDYTLKPVIRIFTAAESGAIAGNIFPLGAAFHVSAITATDTFGTSINADGSFLI
                     GGLVDGSYDVKFSAVAGFTDITVPGVLVTNGSVTQIGEIIIPL"
BASE COUNT         2476 a         1349 c         1408 g         2395 t
ORIGIN      
        1 aacaggtggt ggttttgttc agcataaaga tgagctggat gctgcgctaa tgcgaggttt
       61 aaccgcttca ccaacgcatg aggttttggt agaaaaggct gtattaggct ggaaagaatt
      121 tgaattagaa ttattacgtg atgctgccga caatgtggtt attatttgtg tggtggaaaa
      181 tctcgaccct atgggtgtgc ataccggcga ctcgataacc gtggctccgg caatgacgct
      241 gagtgatacc tgttaccagg aaatgcgtaa tctttcgata aaaatgatgc gcgcaatggg
      301 caattttgcg ggtggatgta atgtgcaatt tgcgcaggat ccttataccg aagaattaat
      361 tgcgattgaa attaatccgc gggtgtcacg ttcatcggca ctggcatcaa aagctaccgg
      421 ttacccgatt gcaaaaattg ctgctaaact tgctattggt tatcgattag atgaattgag
      481 taatcagatt acaaaatcta cctccgctta ttttgaaccg gctttggatt atgtggtagt
      541 aaaaatgcct cgttggaatt ttgataaatt tccgggtgct gatgatacat taggattaca
      601 aatgaaaagt gttggtgaag taatgagcat cggccgcagt tttcaggaag cattacaaaa
      661 agcttgtcag agtttagaaa ataataaaat tggtctcggt ggcgaaaaag gtttttacaa
      721 atcgagtcag gaaattattg acagtttagc aaaaccacat tgggaccgca tatttaatat
      781 taaaaaagcg atggacttag gtgtgcctgt gcgcaccatt cacaaccaaa gtttaattga
      841 cccatggttt ttgatgcaaa ttcaggaact ggtgaaaatt gaaaaagaag tccgccgtta
      901 ccagttaaat aatattccgc ataaattatt acttacctta aaacaaaaag gttacagcga
      961 tttacaaatt gcaaataatt taggtaattg cactgaagaa gaagtatata accaccgacg
     1021 tagtttaggc attaaacgca cttacaaatt agtggatact tgtgctgcag aatttgaagc
     1081 aaaaacacca tactactact ctacttttga agatgaaaat gagagtgtaa gcagcgacaa
     1141 gaaaaaagta attgtattag gttcgggtcc aaacagaatt ggtcagggta ttgaatttga
     1201 ttattgttgt gtgcatggtg tattagccat taaagaaagt ggttatgaag ccattatggt
     1261 aaattgtaat cctgaaactg tttcaaccga ttttgatatt gccgataaat tatattttga
     1321 accggtgttc tgggaacatt tatgggaatt aattgaacat gaaaaaccgg aaggtgtaat
     1381 tgtacaattg ggcggacaaa ctgcattaaa acttgcgaaa caattacacg aaaaaggaat
     1441 taaaattata ggcacttctt acgacaatat ggatattgcc gaagaccgcg gaagattttc
     1501 tgatttatta aaagaattag gcattcctta tcctgaatat ggggctgcaa caactgttga
     1561 agaagcaaca gaagtagcac atcgcattgg ttatccggta ttggtgcgtc caagttatgt
     1621 attgggcggg cagcgtatgc gaattgtaat taacgatgaa gaattagaac gcagcgttat
     1681 cggaatttta aaacattttc ctgacaaccg tatattaatt gaccatttcc ttgaccgtgc
     1741 tgaagaggcc gaaattgatg ctatttgtga tggcactgat gttcacgtaa tgggtattat
     1801 ggaacatatt gagccggcag gtattcacag tggtgattca agtgcggtat tacctccatt
     1861 tcatttaagt gcacatgtga ttgcacaaat ggaagattac acaacaaaaa ttgcacatgc
     1921 tttaaatatt cggggattaa ttaatattca gttcgcgatt aaaaatgaaa aagtatatgt
     1981 aattgaagcc aacccacgtg cttcgcgaac aactccattt attgcaaagg catatcaaat
     2041 accttatttg aatgtagcaa caaaagtgat gttaggcgtt aaaaaattat cagactttac
     2101 ttacgataaa aaattaaccg gttatgcgat taaagaacca gttttcagtt tcgaaaaatt
     2161 ccctaatgta aataaggaat tagggcctga aatgaaatcg accggtgaag ccattcgttt
     2221 tattgccgac ttaaccgacc ctgctttcag agaaatgtat tcgaaaaaga gcatgtattt
     2281 gagcaggtaa gagggctgag agatgagaga tgagcaatga gcaatgagtg atgagtgatt
     2341 tttggattgc ttgacattta atatttttca ttgcattttg tctattactt gcattggcta
     2401 attaaaaatt ttatgattcg ataaaccact catcactcat gtccgcctaa gcggacaact
     2461 cattactcat ttaatgaggg attttcggat ttctggacaa ttattaaaac cacatccaat
     2521 tcagttgttg tgcaatgaat accaattcag gattgattat tatctttaca gtcaataaaa
     2581 cagttaggta tgtctgatat attttggata atattaatct taggacttta ttggtatatt
     2641 atcagaccga tgatgcaagg aatattggag caaaataagt cggcgccatc cggcaggcag
     2701 caatctccat ctacccccaa aaaacaacag tccgaaaaac gtgatggtga gtatgtagat
     2761 tacgaggaaa taaaataatt acctcaaacc ccacaatatt tttttatacc atcgttgtaa
     2821 ataaaacgaa ctgattctaa aataattggt acgtctgtta ctgatggtgg aatccaatct
     2881 gccacttata cttccctgaa tggcaatagt aggatgtatc caaaacatat taattatttg
     2941 tttattgata caatggttat gataccagtc aattgcctga tcagttttgt tttgagctaa
     3001 atccgctaac atattacttg cagctttgta atccaccaaa taggctccgg ccattctacc
     3061 tcgcgagttg ggatataaca aagttccttt ttttctttta ctgcccttta catatcgcaa
     3121 gctggtttct tcaagactaa tgatataatt ttctagttgc ctggttttta tttcggactt
     3181 acattgctgc agtttttccg aaaaatgttt atccaaataa atatcgtctt cgaaaataat
     3241 agcttcaggt agttccaacc tcaccatttc ttcataggct ttgatgtgtt tataggcaca
     3301 tgaactggca ttacttacaa ctgccatttt acctgtaaag tagttggcca atagtgaggg
     3361 attcaactca tgtttatcgc cttccaatat aaactcagca gacaagttgg ctttaaccaa
     3421 ttcttgttgc atatgtttta atctgtggtc cacacctttt acgtgtatta caaaagtttg
     3481 catattgttt tttcagtgta gtaataagtt gcaccaattt ccaacaaaaa aattcacctt
     3541 ctttatttaa actcgttgta tacaggttgg tttatttccg gtattaaaca agactatcca
     3601 ttcactaagt taaacatttg taacaaaatg cgatttgaaa gcagcgccat gcaaaaattg
     3661 aattaagaag ccgaaacaga aatgctgttt cggcttacaa aaaagttaag atgtgattac
     3721 attcttacag taacccctaa ttttatccag gcgacatcat accctaattc agcgtaagcg
     3781 gctaaattgt cggtaaagaa atatttagca cctgcacata aacccccgta taaaccaaca
     3841 ctggtactgt tatttccaac tgttaaaggt gaatcggaag aataatcgta cgtttcaact
     3901 accaccccta aatttactac tccgtaaata tccactttag gaatatcgag ataaacatgg
     3961 tatgacccgc gtcctccaac aacgaaatct ttccaggagc ctttgtcggt atatccaaaa
     4021 taattatatt cataggatga ggatttataa cctaataaac cgccaatacc aatagttccg
     4081 ggacccacat catccatata ggcataatca agtgagacag tcagtaatgg tgaagttttg
     4141 aaatcgccga aagaaacata cgataagtag ccacctatac caaagcctac attaacattg
     4201 atattaccgg ccataaattc ctgtgcgtgt gttttattgg ttgaagcggc cattattatg
     4261 gtagcaacaa caagtaacaa tttgtttttc ataaatttta gtttcaagtg caaagttatt
     4321 acccacagat aaggaaaaaa gtgattttta tccacaaaat atattttttc ccagcaattt
     4381 tttttcattt ttctactatt tcatacagac tataaattgc gataatcgcg aaaaacagat
     4441 gtaacctttc tgggagttgc aaaaaatgca aatgaaataa aaattacttg caaaaagccg
     4501 tttcagcagc agtgtttgaa cctacacctt tgttaaaaaa aatatgtcca caaaaccaag
     4561 gtatgccaat gccaataatg aagaaaaagt atttattgat acattgcgca aaaaggtgaa
     4621 ccagtatttt aaagttcaac atttgcctaa acacgcaggt aacaagctct tattaagagg
     4681 gcttatttta ttagtgttat tattgataat caggacatta gcagttattt cggcaactca
     4741 aatcggcttg ttttatttca gctatgtgtt aacaggaatt attgctttac ctattatcct
     4801 aaacattggc catgatgctg tccataataa tttttcaggt aaccctttca tcaataaact
     4861 agggtcaggg gtcttttatc tttttggaac aagcacctac ttttggaaac tcaggcatat
     4921 ttatgcgcat catgccttca ccaatgtgcc tggttgggac cttgatatca cacaatctaa
     4981 catcattcga ctacacgatg ggcagaaacg caaaccattc caccggtttc aatatttgta
     5041 tatgccattt ttattcagtg tttatacgtt aaactggttt ttttaccgcg atattaaaga
     5101 tatattctgc cacaaattcg gtaataaaac aatagacaaa cacgcaagca cccaaattgt
     5161 attattgatt gtttcgaaat tatttttcct tttttacatg ctggttgttc ctatggtttt
     5221 tcagcatgtt gagctgcatg aagcattgtt tgctttttta ctatttaatt tgagtgcagg
     5281 tttaactgtg acacttgcat tagtaagtac acatgttgga gaaagccagg aatttgtcgc
     5341 tataacagat aatacattac cctattcatg ggagatgcac caattgcaaa ctacagcaga
     5401 ttttgccaca cgaaacaaat ttatcacctg gttttttgga ggatttaatc accacgttgc
     5461 ccatcatatt tttcccggca tttcacatat ccattatgca gcagttacgc caattattaa
     5521 agaaacaact gcggcatttg gaattactta ccacgaatcg cccaccttac ttacagccat
     5581 ccgtgagcat ttcaccttac ttaaaataaa aggtctaaaa gaagaatttt aatttaaatc
     5641 aaataactat gagaatagcc actattgccg acagagcaac agtagtaaac attattgttg
     5701 ccatgtttaa acacaaccca accatacagt ttatggtaaa aaacgataac aaagcaaata
     5761 aaagaataac agcaattgcg gaatatgcct ttgattttgc agttgctcgt gatggggtat
     5821 atatttctga caataacaaa ggggttgcca tttgttacca gtataattta aaagctatca
     5881 cgctaaagga tattggcctt atgcttaaaa tggtattcaa agccatacat atcagcaaat
     5941 tattccagat ttttcttcac gataatttta tcaaatcgca gcggcctgta gatggcaatt
     6001 attattattt ttggtttttt ggtgtgctgg cagaagaaca gccaaaaatt tcggcacgag
     6061 atttaactat tgcattattt gaaaaagcca aaatcaaacg catcccgatt tatgcagaaa
     6121 ccacgcttga aaaaaataaa aaagtgtatg agcgatttgg atttaaagta tataaaacat
     6181 ggataaacca aaccaatggt atacaggttt ggttcatgtg ccgcgacttc cggatggcat
     6241 aaaattaaat ccgaagaaat agcaacctgt tatttaaaca ggttgcttac tttttcttta
     6301 atatttttag cagcttcttc cgttttttca actacatctt ttagtacttc ctgtgatttt
     6361 tctttggctt caacagctgc atccataaat gtttctttca aactatctga aaatttttca
     6421 cctttcagca tattgtcaat ttggctgtgc aacgatttag gcatttttga tttaaggtat
     6481 tcagctacag tattgatact attttttgcc tgttcatcgg taataccgct ggattgtttc
     6541 actttgtcaa ttatttcttg cataaatgtt gattttattt tatccaattc atttaataca
     6601 accaaaatac aaaaattata cccattaaaa aaatatttta aaaaataaac gaataccggc
     6661 tccttttggg gaaaatatcc ccacaaaaat tattcttgcc agtgctgaaa cctagacaga
     6721 taaggctttt tacaggttgg tataggtatt ggactattat aaaaaaaata atgatatgag
     6781 aaagcgaaat gtatggccag ttataattgc agcaatagca ttaattggta caataacgat
     6841 gacctcttgt aacaaggata atcaggatcc aacaggaacg gcgcatgtta atatgtactt
     6901 aaccgatgct cctgccgatt atgatgcggt gtatattgat gtacaatcaa tagaaattat
     6961 ttccgatgca ggaactacca ctcacacatt attacatccc ggtgtatatg atttgttaaa
     7021 atttaattcc ggggtggata cacttatgat ttctgaagat ttatcaccaa gaacagtatc
     7081 gcaaatcaga ttgattttag gtgaaaacaa cagtgttgtt gtagatggtg ttagttatcc
     7141 attatccaca ccttcagcgc aaacttcagg attaaaactt aatgtgcatt atacctttga
     7201 agcaggtata tcttacaatt tatggatgga ttttgatgct tcgcaatcga tagttgaaca
     7261 gggtaatggc gactatactt taaaaccggt gattcgcata ttcacggctg ctgaatcagg
     7321 tgcaattgct ggaaatatat tcccgttagg cgcggccttt catgtaagtg caattacagc
     7381 cactgataca tttggcactt cgattaatgc agatggtagt tttttgatcg ggggattggt
     7441 tgatggttcg tatgatgtaa aattttcggc agttgcagga tttacggata taaccgttcc
     7501 gggtgtattg gtaaccaatg gttcagtaac ccaaataggc gaaataatta tcccattgta
     7561 atacattcac atcaagtaaa caacccccac caaaggtggg ggcttttata aaaaattaaa
     7621 cccccgat
//
LOCUS       DAORKW010000121         7626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_290_length_7626_cov_5.210540, whole genome shotgun sequence.
ACCESSION   DAORKW010000121 DAORKW010000000
VERSION     DAORKW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_290_length_7626_cov_5.210540"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLB46_06485"
     CDS             <1..214
                     /locus_tag="PLB46_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76199.1"
                     /translation="NFQILSGATIMLQIMDVSGKIVDTYANIPVSNPGETIRFNVYRK
                     NLVAGYYQAMVKYNNVNFGDFKIIVN"
     gene            316..927
                     /locus_tag="PLB46_06490"
     CDS             316..927
                     /locus_tag="PLB46_06490"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRF76200.1"
                     /translation="MSNRSPQVDQVILKAAAFAQPILTHFRDLVHAVCPEVEEKIKWG
                     MPFFVYNDDNLCHMAAFKQHCAIGFWKAKLIDEKLVAKAKTEEAMGHFGKITSLKDLP
                     SDKLLTKWIKAAMQLNDAGIKLTKPPVEKNKPVEVPADIQKVINKNAKAKKTWIAFSN
                     SHRKEYIQWITEAKTSATKEKRITTMLQLLEEGKDRYWKSAKK"
     gene            complement(973..4023)
                     /locus_tag="PLB46_06495"
     CDS             complement(973..4023)
                     /locus_tag="PLB46_06495"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRF76201.1"
                     /translation="MYKSVIAFITILVLNLHLNLHAQSTGYWQQNVDYDITVSLDDIN
                     HVLNGNISMQYTNNSPDALTFIWIHLWPNAYKDNNTAFAKQKVLQGSTEFFFAKDNER
                     GYIDNLDFKVNGTSVPLTYDPENPDIAKITLNSPLASGQTITITTPFKVQMPKCVSRM
                     GHVGQQYQVTQWYPKPAVYDKHGWHPIPYLDQGEFYSEFGNFKVSITVPENYVVAGSG
                     DLKTASEFTWLNALADKTASTELTETSLDFPPSSTTTKTLQYELKNSHDFAWFADKRF
                     HVMRSSVVLPESERTVLTYVYFTNQHKTEWKKACDYVNRSVLFYSDNVGEYPWNIAQA
                     VDGTLEVESAGGMEYPTVTVLGGDYDAESLDNVITHEVGHNWFYGIIGSNEREHAWMD
                     EGINSFYENRYMDTYYTRSNALGIPNNFLGYLGVDTNNVDNVQYHMYQSMARQNKAQC
                     INLHATEYTMINYGLDVYMQSAYIMRYLEDVLGKEEFDRIMRKYYRTYQFKHVYPEDM
                     RALFEAETGEYFAWFFDGVINDARGVDYKISKMQKGKTAIAVDITNKSDIPAPFSVAV
                     MDGDSILRTDWFRGFTGSQTLYFNIKPEWKVSHLKIDAQGTIPEYERENNTIKTSGLF
                     KRVEPFKLTLLGLKVDNPNKTTVTLSPVIGWNENDKFMLGLSLWNSVLPTPAIDFVLA
                     PMYAFGTEKLVGQGSIGYNLYPAKGFIDRLRISESVASYTYNKYEVLTVGESTTLTPS
                     YTRLQSKAEIDIRKKHLSQKHKHSFAFRNVFIQEDDYYFELGDNGEITNTFAEQYMVN
                     ELSYRFNKPSKIFPQSLFVAGEMGDDYSKISFTYNTKIHYPGTTTGVDVRVFAGTFLQ
                     NGESDKHYFTLAGNNGYYDDMYDEIYLGRNTQNMFISNQISLSDGFFKVPVYDLGLTS
                     DGAIAAANLDIAIPRIPLVGLFGDIAYVSPNTEVVGFDAFQYDAGIMIKLPKNIFAMY
                     FPLLMSENLSNQFVDDVNTYGDKISFMLSLNALNLFEIARKMEF"
     gene            4150..5565
                     /locus_tag="PLB46_06500"
     CDS             4150..5565
                     /locus_tag="PLB46_06500"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF76202.1"
                     /translation="MMDPEFNDREFTEMGELIELFEKYLKSNEQHFFDEDSLERILEY
                     YEMRNLPERAEAVADYAINQNPYSSDFLVRKAEFLVNRKKYNEALDLLDKAYLFDSHE
                     IDIYLLRSDIYIETNQPEKAEEILFEALEMADDEEKDAVYAELSDIYEIQENFDKAFD
                     CLAKALSINPSSEDALYKLSHIVDMTDKYDESVALHLSIIEKEPYAWLAWFNLGRAYM
                     GLGLYEKAIESFEFVMAIDENFDLVYRDAADVYYRIENFEKAISMFELAHEKSGGYED
                     YSFRIGLCYERLENYKSARFHFRKAARQDPFLSEAYFRIGETYRQEDRFEPALVNYKK
                     ALKLDDSNEDYICTLISIYKMLDRDEDVIFYLKTLVKTRPDILSYWLDYIIYLMELAK
                     YEEVIDVIHDAITLSGHYPEYFYLQSAALFNTGKSREAISVLEHALTMDYSRHTILYE
                     VCKEVAMRPEFQKVIGLYNAN"
     gene            5684..6442
                     /locus_tag="PLB46_06505"
     CDS             5684..6442
                     /locus_tag="PLB46_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HRF76203.1"
                     /translation="MNFSINSLPERHTKPRKEGLTMVMDKGLSVREAEDMLSVSEPYI
                     DLIKLGFGTAFVTPNLENKLAVYKNAGIACYFGGTLFEAFYIRGQFDDYLRLLEKYKM
                     GYAEVSDGSIDMDHDEKCKYITALSKHVTVLSEVGSKDENKMLAPYKWIELMKKEIEA
                     GSYKVIAEARESGTVGIYQSKGEVRSDLIDEILTQVPQEKILWEAPLKAQQVYFIKLL
                     GSNVNLGNIPPNEVIPLETLRLGLRGDTFHHFLK"
     gene            6465..7400
                     /locus_tag="PLB46_06510"
     CDS             6465..7400
                     /locus_tag="PLB46_06510"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HRF76204.1"
                     /translation="MSLIKKYFPLTLKGIAMGAADVVPGVSGGTIAFISGIYEELLNS
                     LKSFNGTAVKKLFKEGFGAFWKHINGWFLLFLVAGIGISILSLSKLILHLLENYPNLI
                     WSFFFGLVVASAVFVAGTIKKWSWDVILAALIGTGIAYYVTVATPSQTPENYGFIFIT
                     GAIAICAMILPGISGSFILLLMGKYEFILNSLNSMKFDIILTFIAGCFLGLIGFSHVL
                     SYMLKKYHNATVALLTGFMFGSLNKVWPWKEVLETQIIDGEEKILREANVLPSTYTQI
                     TGEPHQALWCILLAVVGFVLIYLIEKMASKKHKII"
BASE COUNT         2472 a         1363 c         1373 g         2418 t
ORIGIN      
        1 taattttcaa atactatcag gtgcaacaat tatgttacaa ataatggatg taagtggtaa
       61 aattgtagat acctatgcca atatccccgt aagtaatccg ggcgaaacca ttcggtttaa
      121 tgtgtatagg aaaaatttgg tggccgggta ttatcaagcc atggttaaat acaacaatgt
      181 aaactttggt gattttaaaa ttatagtcaa ttaacacgtt tattaaaatt accaggtgac
      241 tctatttgtg agttttttgt taaaattgca tagctttttc aattgataaa ggcaatttaa
      301 atcaaaacgc taaacatgtc taatcgaagc ccacaagtgg atcaggtaat tttaaaagcc
      361 gccgcatttg cacaacctat tctcacacat tttcgtgact tagtgcatgc cgtttgtccg
      421 gaggtggaag aaaaaattaa atggggaatg ccattttttg tttataatga tgataacctc
      481 tgtcacatgg ctgcattcaa acaacattgt gcaataggat tttggaaagc aaaattaatt
      541 gacgaaaaac tggttgctaa agcaaaaacc gaagaagcca tgggccattt tggtaaaata
      601 acttccctaa aagatttacc atcagataaa ttattgacca aatggattaa agcagccatg
      661 caactcaacg atgccggaat caaactgacc aaaccaccgg ttgaaaaaaa taaacctgtt
      721 gaagttcctg ctgatattca aaaagtaatc aataaaaacg caaaggctaa aaaaacatgg
      781 attgcattta gcaactcaca ccgcaaagaa tatatccaat ggataacaga agcaaaaaca
      841 tccgcaacta aagaaaagag aataactaca atgctccaac tacttgagga aggaaaagac
      901 agatattgga aatcggctaa aaaataataa gtctcgtcgg ggcgaaagat tattcgcccc
      961 gacagcgtct aattaaaatt ccatttttct tgcaatctca aataaattca atgcatttaa
     1021 actgagcata aaacttattt tatctccata agtatttaca tcatctacaa actggttact
     1081 taagttttca ctcatcaaca atgggaaata cattgcgaaa atatttttag gcagtttaat
     1141 cataatacct gcatcgtatt gaaatgcatc aaaaccaaca acttctgtat ttggcgacac
     1201 ataggcaata tcaccaaaaa gtccaacaag cggaattctt ggtatggcaa tatctaaatt
     1261 ggctgcagca attgcgccgt ccgacgttaa tcctaaatca taaacaggca ctttaaaaaa
     1321 tccatcactt aaacttatct ggttgctgat aaacatattt tgggtattac gtcctaagta
     1381 aatttcatca tacatatcat cgtaatatcc attatttccg gcaagcgtaa aatagtgttt
     1441 atccgattct ccattttgta aaaaagtacc ggcaaatacc cttacatcaa cacctgtagt
     1501 agttcccgga taatgaattt ttgtattgta agtaaacgaa atttttgaat aatcgtcgcc
     1561 catttctccg gctacaaata aggattgtgg aaatattttt gacggtttat taaagcgata
     1621 cgacagttcg tttaccatat attgctctgc aaacgtatta gtaatttcac cattatcgcc
     1681 taattcaaaa taatagtcat cttcctgaat aaatacgttc ctaaatgcaa agctgtgttt
     1741 gtgcttttgg cttaaatgtt ttttgcgaat atctatttct gcttttgatt gtaatcgtgt
     1801 ataggaaggc gttaaagtag tagattcacc tacagttaat acttcatatt tattgtaagt
     1861 ataacttgca acactttccg aaatcctcaa cctgtcgata aaccctttgg caggatacaa
     1921 attatatcca atacttccct gaccaactag tttttcagta ccgaatgcat acatgggtgc
     1981 taaaacaaag tcaatagccg gagtaggtaa aaccgaattc cataacgaca aacctaacat
     2041 gaatttatca ttttcgttcc agccaattac cggcgataaa gtaactgttg ttttattcgg
     2101 attgtccact tttaatccca ataatgtaag cttgaatggc tcaacacgtt taaataatcc
     2161 tgaagtttta atggtattat tttcgcgttc atattcagga attgtgcctt gtgcatcaat
     2221 ttttaaatgc gaaactttcc attcgggttt aatattaaaa tataatgtct ggctacctgt
     2281 aaatcctcta aaccaatcgg tgcgtaaaat actgtcccca tccattactg ctactgaaaa
     2341 tggtgcaggt atatccgact tgttggtaat atcaactgca atggcagttt tacctttttg
     2401 cattttactt attttgtaat ccacaccacg agcatcatta atcacaccat caaaaaacca
     2461 tgcaaaatat tcgccggttt ccgcttcaaa cagcgcacgc atatcttccg gataaacatg
     2521 tttgaactga taagtgcgat aatattttcg cattattctg tcaaattctt ctttgcctaa
     2581 aacatcttct aaataacgca taatataagc actttgcata taaacatcaa ggccataatt
     2641 aatcatggta tattccgttg catgtaaatt aatacactgt gctttatttt gacgagccat
     2701 tgactgatac atgtgatatt gtacattatc tacattatta gtatctacac ctaaatatcc
     2761 taaaaagtta tttggaatac ccagtgcatt tgagcgggta taataagtgt ccatgtatct
     2821 gttttcataa aatgaattaa ttccttcatc catccaggca tgttcacgtt cgttactacc
     2881 aattattcca taaaaccagt tgtggccaac ttcgtgtgta attacattat ctaacgactc
     2941 agcatcgtaa tcaccaccta aaacagtaac agtgggatat tccataccgc ctgcactttc
     3001 cacttctaaa gtaccatcaa cagcttgcgc aatattccac ggatattcac caacattatc
     3061 agaataaaat aataccgaac gatttacata atcacaagct tttttccatt ctgttttatg
     3121 ttgattagtg aaatatacat aagtaagtac agtgcgctcc gactccggca ataccactga
     3181 actgcgcatt acatgaaaac gtttatctgc aaaccatgca aaatcatgtg aattttttaa
     3241 ttcgtattgc aatgtttttg ttgttgttga agaaggagga aaatctaaac ttgtttctgt
     3301 taattcagtt gaagcagttt tgtcagctag tgcatttaac caggtgaatt cagaagctgt
     3361 ttttaaatca ccactaccgg caactacata attttcaggt actgtaatgc ttactttaaa
     3421 attgccaaat tcactataaa attcaccttg gtccaaatag ggaattggat gccagccatg
     3481 tttatcatat acagccggtt ttggatacca ttgtgtaacc tgatattgtt gcccaacatg
     3541 tcccatcctg gaaacacatt ttggcatttg tactttaaat ggtgtggtaa tggttatagt
     3601 ttgtccgctt gccaaaggac tatttaaagt aatttttgcg atatccggat tttcagggtc
     3661 atacgttaaa ggaaccgaag taccatttac tttaaaatcg agattatcaa tataaccgcg
     3721 ttcattgtct ttcgcaaaga aaaactcagt acttccctgc agcacttttt gtttggcaaa
     3781 tgcggtatta ttgtctttat acgcattcgg ccataaatga atccagataa aagtgagtgc
     3841 atcaggggaa ttattagtgt attgcatgga aatattgcca tttaatacat gattgatgtc
     3901 gtcgagggat acagttatat cataatccac attttgctgc caataacctg ttgactgcgc
     3961 atgaagattt aagtgtaaat tgagcaccaa tatggtaata aaagcaatca ctgatttata
     4021 cataggttaa atttttggta aagatacagg ttccgttgtt tgcttcgtta atgaaacaaa
     4081 cagccaaaat gttacagaat tgttgatcaa cattgaaact aaatttggaa aaactaaatt
     4141 tgcatgtata tgatggatcc ggagtttaac gacagagagt ttactgaaat gggcgaattg
     4201 attgagttgt ttgaaaagta tctcaaatcc aatgaacagc atttttttga tgaggacagc
     4261 ctggagcgta ttttagaata ctacgaaatg cgcaatttac cggagcgtgc tgaagctgtt
     4321 gccgactatg ccatcaacca aaacccttac tcctccgact ttctggtgcg taaagctgaa
     4381 tttctggtaa atagaaaaaa gtataatgaa gccctcgatt tattggataa agcctacctg
     4441 ttcgacagtc atgaaataga tatttattta ctgcgctccg atatttatat agaaaccaac
     4501 cagccagaaa aagccgaaga aattttattt gaagcgttgg aaatggctga tgatgaggaa
     4561 aaagacgcgg tttatgctga attgagtgat atttatgaaa ttcaggaaaa cttcgacaaa
     4621 gcgtttgatt gtttagccaa agccttgtct ataaaccctt ccagcgaaga tgccttgtat
     4681 aaattatcgc atatcgtgga tatgaccgat aaatatgatg aaagtgtagc cttacatcta
     4741 tccattatag aaaaggaacc ttatgcctgg ctggcttggt tcaatcttgg tagagcctat
     4801 atgggactag ggttgtatga aaaagcgatt gaaagttttg agttcgtaat ggccatagat
     4861 gaaaatttcg acctggtgta tcgcgatgca gccgatgttt actaccgtat cgaaaatttc
     4921 gaaaaagcga tttccatgtt cgagctggcg catgaaaaat ccggtggtta cgaagactac
     4981 agctttagga ttggtttgtg ttatgagcga ctggaaaact ataaatctgc ccgttttcac
     5041 tttagaaaag ctgcccgtca ggacccgttt ttgagcgaag cctactttcg aataggagaa
     5101 acctatcgcc aggaagaccg ctttgagccg gctttggtca attacaagaa ggctttgaag
     5161 cttgatgaca gcaatgaaga ttatatctgc acccttatca gtatctacaa aatgcttgat
     5221 cgcgatgagg atgttatttt ttatttaaaa acactggtta aaacccgtcc tgatatactg
     5281 agttattggc tggattacat tatctatttg atggaacttg ccaaatatga ggaagtaatt
     5341 gatgtcattc acgatgccat taccttaagt ggtcattacc cggagtattt ttatctgcaa
     5401 agtgccgcct tgtttaatac cggcaaaagc agagaagcca tttcagtcct ggaacacgca
     5461 ctcaccatgg attacagccg acataccatc ctttacgaag tgtgtaaaga agtggctatg
     5521 cgacctgaat ttcagaaggt gattggcttg tacaatgcta attaacaacc tgctaaacct
     5581 aaatttttgc tgttttcaat taaaagcatt gaggttgcaa tagttatact tcatactatg
     5641 ccttcgttta cgtaattttg cgccgtaact actaatttaa taaatgaact tttctattaa
     5701 cagtctccct gagcgccata caaaaccacg caaagaggga cttaccatgg taatggacaa
     5761 aggcttaagt gtgcgtgaag ctgaagatat gctatccgtt tctgagcctt atatcgactt
     5821 aattaaactc ggatttggaa ctgcgtttgt aacgccaaac cttgaaaata aactagccgt
     5881 ttacaaaaat gcaggtattg cctgttattt cggcggaacc ttatttgaag cattttacat
     5941 tcgcggacag tttgatgatt atttgcgatt gctcgaaaaa tacaaaatgg gttatgctga
     6001 agtaagcgat ggctctattg atatggacca cgatgaaaaa tgtaaataca ttacagcatt
     6061 aagcaaacac gtaactgtgt taagtgaagt tggttctaaa gatgagaaca aaatgcttgc
     6121 tccatacaaa tggattgagc tgatgaaaaa agaaattgaa gccggaagtt ataaagttat
     6181 tgctgaagca cgtgaaagcg gaactgttgg tatttaccaa agcaaaggtg aagtgcgcag
     6241 cgatttaatt gatgaaatat taacgcaggt accacaggaa aaaattcttt gggaagcacc
     6301 acttaaagca cagcaagtct attttatcaa attattaggc tcgaatgtaa accttggtaa
     6361 tattccacct aacgaagtta ttccactgga aactttacgt ttaggattac gcggagatac
     6421 cttccatcat tttttgaaat aacgaattgt tataaattac tctgatgagt ttaataaaaa
     6481 agtatttccc acttacatta aagggtattg caatgggcgc tgcggatgtt gttccgggtg
     6541 tttccggagg aaccattgca tttatttcag gcatatatga agaactactc aattctttaa
     6601 aatcttttaa tggcactgct gttaaaaaat tatttaaaga aggttttggc gctttttgga
     6661 aacatattaa cggatggttt ttattatttc tggtggctgg tattggaatc agtatcttat
     6721 cgctatcgaa attaatttta catctgttag aaaattatcc aaatttaatt tggagtttct
     6781 tctttggctt agttgtagct tcagcagtgt ttgttgccgg cacaattaaa aagtggagtt
     6841 gggatgtaat tcttgcagca ttaatcggaa caggtattgc ttattatgta acggtagcaa
     6901 caccttcaca aacaccggaa aattatggtt ttatttttat taccggagct attgccattt
     6961 gtgcaatgat tttacccggc atttcaggaa gttttatttt attgctaatg ggtaaatatg
     7021 agttcatttt gaattcatta aactccatga aatttgatat catcttgact tttattgccg
     7081 gatgtttttt aggattaatt gggttttcgc atgtattaag ttatatgctc aaaaagtatc
     7141 ataatgctac tgtagctttg ctgacaggat ttatgtttgg ttcattaaat aaagtttggc
     7201 cctggaaaga agttttggaa acccaaatta ttgatggcga agaaaaaata ttacgagaag
     7261 ctaatgtttt accgtctacc tacacacaaa ttaccggtga acctcatcag gcattatggt
     7321 gtattttatt agctgttgta ggttttgtgt tgatttattt aatagaaaaa atggccagca
     7381 aaaaacataa aataatttaa tagttgatat gacaatacca caacctcaac ctgaccctct
     7441 ccccaacttt acaattaaaa tccgaattaa atgaaactat tcggtttaat tggctttccc
     7501 ttatcccatt ctttttctga gaaatttttt tctgagaaat ttgaacgtga agaaattgaa
     7561 gattgtaaat acgaattata tccactcgag aatatcgaag acattcgttt gttgttcgaa
     7621 gtaaat
//
LOCUS       DAORKW010000122         7606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_294_length_7606_cov_4.650245, whole genome shotgun sequence.
ACCESSION   DAORKW010000122 DAORKW010000000
VERSION     DAORKW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_294_length_7606_cov_4.650245"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /gene="rodA"
                     /locus_tag="PLB46_06515"
     CDS             complement(<1..985)
                     /gene="rodA"
                     /locus_tag="PLB46_06515"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRF76205.1"
                     /translation="MQNIDQFAIGLYFALLVIGLLTVFAVSYDPETSRFFNFSQTHGK
                     QMVWMFISLIVGFSILTFDSTFFTKFAMIFYAVTLGIFILTFLVAVDINGARSWLQVG
                     SFQFQPAEFGKTTTALMLAKFMSMITGQARTFKNKLIAYLILGVPMGIIVLQSDVGSA
                     MVYGSLVFVIYREGFPVYEVLFVLFIGLCFVFSMIFGGDPVAITLSIAILIYVGFSSL
                     KLIRKEKVKGIAFLATFAGAILLFLLGGEVMSLNMYLIYAGTIGLLFASWYMVRKIKY
                     FKIWWPVVIYVALLAFVIFGVKYITTSLLEDYQVQRIETLLGMSDNADAEYN"
     gene            complement(1003..2919)
                     /gene="mrdA"
                     /locus_tag="PLB46_06520"
     CDS             complement(1003..2919)
                     /gene="mrdA"
                     /locus_tag="PLB46_06520"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRF76206.1"
                     /translation="MAIIDNTKSRSYVIQGIIVGIFVVYLIRLFYLQIIDDTYKQIAD
                     DRGIRKEQVDPDRGQIFDRTGKLIVYNEPSYKLMVVPSQVEKIDTIRFCEIIGIDKEQ
                     FIKKMKDAKSFSRVRESVFLSQISAEEFGRIEEYLYEFKGFYTAISPVRKYAYPSAAH
                     LLGYVAEVDSNDIKKSNGYYRNADVIGKSGVEKSYENLLRGQKGYKNIVIDRFGRDVG
                     RLNNGSEDIAPIAGKNLTLTIDIELQTYAEQLMANKRGALVAIEPSTGEILALVSTPS
                     YDPNMLVGSARTKNFAKLALNKENPLNNRALSGYYPPGSTFKPVMAALGMDIGSLTSD
                     QGYSCPGGYVMSGHKVNCHNHPYASNVELGIGHSCNAYFCYTFRYFMEAHDSPEEGMR
                     DFNEHLALWGIGTKTGVDLPNERNGNVPDAEDYDKVYGKGRWKASNCVTLGIGQDKLI
                     ITPLQSANSMAIIANGGFFFTPHVIKYAEDADTILNKFKTRRETGIPDSIFPFVQHGM
                     AGAVNFGTAKIAKVDSIQVCGKTGTAENPHGKDHSWFSCFAPEKNPKIAVAIIIENGG
                     WGASYAAPIASLVVEKYVNRTISEKRKPLEERMMKAVLIDAPELLPQPEQKTPVDSNK
                     QKITAQAAILPKKQ"
     gene            complement(3023..3478)
                     /locus_tag="PLB46_06525"
     CDS             complement(3023..3478)
                     /locus_tag="PLB46_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76207.1"
                     /translation="MLNKLNVSTYIHPYVYPMFIILLPFETPRWLILPLAFVVGFSID
                     MFNNTAGMHAAACVFIAYARPWLIKRYTPITGYENVNAPSLSELGVVWFALFTLTVIF
                     IHHSIYYFLQVFWMNDVGYLLLKIFLSTLVSTLLIVIFAFLFAKRKVKR"
     gene            complement(3531..4364)
                     /gene="mreC"
                     /locus_tag="PLB46_06530"
     CDS             complement(3531..4364)
                     /gene="mreC"
                     /locus_tag="PLB46_06530"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRF76208.1"
                     /translation="MRSFFLFIIRHYAVFLFGLLELISLYFVFTYNNFHNTFFINSAN
                     GVSGNVLNTYGNFQEYFSLKEVNDSLMHENALLREQLLLSALPDSSSVWVKDSLGQPL
                     YSYIPAEVIGNSFTEPNNYITLNKGSNDGIRENMGVITSSGIVGRVVKVSPKFSVVMS
                     VLHSQFVSRVAVQKNNSQGRLTWEGKSPTHISVIQVSEPGTLNKGDSVVTTKISPMFP
                     PDIMVGTVESFGKDPGSNYYTLNVRLSTKFSSLQFVYVVNELLQKERTDLENSAMDAG
                     N"
     gene            complement(4380..5405)
                     /locus_tag="PLB46_06535"
     CDS             complement(4380..5405)
                     /locus_tag="PLB46_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRF76209.1"
                     /translation="MGIFNFLTQEIAIDLGTANTLIIAHDKVMVDEPSIVAIDRKTEK
                     VIAVGKRAMQMHEKTHENIKTIRPLRDGVIADFQAAESMIRGMISMIFPKNKLFKPSL
                     VMVICIPSGITEVEKRAVKDSAEQAGAREAYLIHEPMAAALGIGLDVQEPVGNMIIDI
                     GGGTTEIAVVALSGIVCDQSIRVAGDEFTADIVDYMRRQHNLLIGERTAEAIKINVGS
                     ALTELDEAPEDYQVSGRDLMTGIPKQLVVTYSEIAHSLDKSISKIEEAILKALETTPP
                     ELASDIQRNGIYLTGGGALLRGLDKRISQKTKLMVHVADDPLKAVVRGTGIALKNREQ
                     YAALLMS"
     gene            complement(5714..7249)
                     /gene="purH"
                     /locus_tag="PLB46_06540"
     CDS             complement(5714..7249)
                     /gene="purH"
                     /locus_tag="PLB46_06540"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRF76210.1"
                     /translation="MHAPIKIKSALISVFYKDQLEPVIQKLHALGVVLYSTGGTREFI
                     EKLGVPVLAVEDLTTYPSILGGRVKTLHPKVFGGILARRELKEDMAQLAQYEIPEIDL
                     VIVDLYPFEETVKSTTVEADIIEKIDIGGVSLIRAAAKNCNDVLIIASKDDYAPLLEM
                     LNEKNGETYFGQRKYFAKRAFEITSHYDDAIYTWFNHNENYGVRYTPKIAMRYGENPH
                     QDAAFYGKLTDVFEKLHGKEISYNNLVDIESCVYLIEEFAETAKKGLSTFAIIKHTNA
                     CGLTTAKTTEEAWDKALAADPVSAFGGVLICNQIIDLATAYKINELFFEVLIAPAFEH
                     DALVVLQTKKNRIILRQKMFEFPAKQYKSMLNGVLVQDRNNKATALQELKYVTEVRPT
                     DAQLQDLLFAEIAVKHLKSNGIALVKNGQMIGCGTGQTSRVDALNQAINKARSFGFDV
                     SGSVMASEAFFPFPDCVQIAHEAGISAVIQPGGSIRDKDSVDYCNEHQMAMVLSGTRH
                     FKH"
     gene            complement(7336..>7606)
                     /locus_tag="PLB46_06545"
     CDS             complement(7336..>7606)
                     /locus_tag="PLB46_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRF76211.1"
                     /translation="PIGPALLPDFGGKGMYGKNVHQAVFDKGVDETGITVHEVDEVYD
                     EGRILFQISIDISPTDTPESIERRVRELEYAYLPGIVEKLLLNNI"
BASE COUNT         2365 a         1619 c         1242 g         2380 t
ORIGIN      
        1 cattatattc agcatcggca ttatccgaca tgcctaataa tgtttcaatt ctttgcactt
       61 ggtaatcttc taacaaacta gtagtaatat acttaacacc gaaaatgaca aaagctaata
      121 aggccacata aattacaacc ggccaccaaa ttttaaaata ttttattttc cgaaccatat
      181 accatgaggc aaaaagtagt cctattgtac ctgcgtatat cagatacatg ttcaaagaca
      241 ttacttcgcc tcctaataaa aacaataaaa ttgcgcctgc aaatgtggca agaaatgcaa
      301 taccttttac tttttctttt cgtataagtt ttaatgaact aaagccgaca taaatcaaga
      361 tggcaataga caatgtaata gcaacagggt ctcctccaaa aatcattgaa aacacaaaac
      421 ataatcctat aaacaagaca aataaaactt cataaaccgg aaatccttcg cgataaataa
      481 caaataccaa actaccatat accattgcag aacccacgtc actttgcaat acgataatgc
      541 ccattggaac accaagaatt aaataggcaa ttaatttatt tttaaatgta cgcgcctgcc
      601 cggtaatcat actcataaat ttggcaagca ttaaagcggt tgttgtttta ccaaattctg
      661 caggctgaaa ttgaaatgaa cccacctgca accaacttct cgcaccatta atatcaaccg
      721 cgactaaaaa agtaagaata aaaatgccaa gtgtaacagc ataaaaaatc atggcgaatt
      781 tggtgaaaaa agtgctgtcg aaagttaata tactaaatcc aacaatgagt gaaataaaca
      841 tccacaccat ttgtttgcca tgtgtttgcg aaaaattaaa aaagcgactt gtttccgggt
      901 cgtaacttac cgcaaataca gtgagcaaac caattaccaa taatgcaaaa tacagtccga
      961 tagcgaactg atcaatattt tgcataatgg attttttttc tctcattgct ttttaggtaa
     1021 aattgctgct tgtgcggtaa ttttttgttt attgctgtca accggtgttt tttgttccgg
     1081 ctgtggaagt aattcaggtg catcaattaa taccgctttc atcatacgtt cttctaaagg
     1141 tttgcgtttt tcgctgatag ttcggttaac atatttttca actacgagac tggcaattgg
     1201 tgccgcataa cttgcacccc aaccaccatt ttcaataatg atagccaccg caatttttgg
     1261 atttttttcc ggggcaaaac aactaaacca actatggtct ttaccatgcg gattttctgc
     1321 tgttcctgtt ttcccgcaaa cctgaataga atccaccttt gcaatttttg cggtaccaaa
     1381 atttacggct cctgccattc catgttgcac aaatggaaaa atactatcag gaataccggt
     1441 ttctcttctt gttttaaatt tatttaatat cgtatcagca tcttcagcgt attttattac
     1501 gtgaggtgta aaaaagaaac caccgtttgc gataatcgcc atggagttag ccgattgcaa
     1561 tggtgtaata attaatttat cctgaccaat acccaatgtt acacaattag atgccttcca
     1621 tcttccttta ccataaactt tatcataatc ttctgcatca ggcacattac catttctttc
     1681 attaggtaaa tccactccgg ttttagtgcc aattccccat aacgcaagat gttcgttaaa
     1741 atcgcgcata ccttcctcag gtgaatcatg cgcttccata aaatatcgga atgtataaca
     1801 gaaataggca ttacaactat gcccaatacc taattctaca ttggaagcgt atgggtgatt
     1861 gtgacaattc actttatgac cactcattac atatccaccc ggacagctat aaccctgatc
     1921 ggaagtgagt gaaccaatat ccatccctaa cgccgccata acaggcttaa acgtagaccc
     1981 cggagggtaa taaccgctta acgctcggtt atttaaagga ttttctttgt ttaatgcgag
     2041 ttttgcaaag ttttttgttc ttgcacttcc aaccaacata ttggggtcgt atgaaggtgt
     2101 gcttactaat gctaaaattt cacctgtcga aggctctatc gcaaccaaag cacctcgctt
     2161 attggccatg agttgttccg catacgtttg caactctatg tctatagtca gcgttaaatt
     2221 ttttccggca ataggagcaa tatcttcgct accattattg agtctgccca catcacggcc
     2281 aaaacggtca attactatgt ttttatatcc cttttgtccg cgcaacaaat tttcataact
     2341 tttttcaacg ccgctttttc caattacatc ggcattgcga tagtagccat tcgatttttt
     2401 aatatcattg gaatctacct cagcaacata acctaacaag tgggctgcag acggatacgc
     2461 gtatttccga accgggctaa ttgcagtata aaacccttta aattcatata aatactcttc
     2521 aatgcgacca aattcttcag ctgaaatttg ggataaaaaa acactttccc tcactcgtga
     2581 aaatgatttg gcatctttca tttttttgat aaactgctct ttatcaatgc ctataatttc
     2641 acaaaatcta atggtatcaa ttttttctac ctgcgaaggc actaccatta atttataact
     2701 tggctcgttg taaactatca gtttgccggt cctgtcgaaa atttgccctc tgtccgggtc
     2761 cacctgctct tttctaatcc ctctgtcgtc ggcaatttgt ttataggtat cgtcaattat
     2821 ctgaagataa aacaggcgga taaggtacac gacaaaaatg ccgacaatta tgccttgtat
     2881 aacataactg cgagattttg tattatcgat aattgccata aattaaattg cctgtgaagt
     2941 tgaatgccga ttccgaattc cggatacaaa tttcgaaaat gtttttacaa taccatcagt
     3001 attgttacag aattaattgt aattaacgtt ttacttttcg ttttgcaaac aagaaagcaa
     3061 aaattacaat taataatgtt gatactaaag tacttaaaaa tatttttaat aataaatatc
     3121 ccacatcatt catccaaaat acctgtaaaa aataatagat actatgatga ataaaaatta
     3181 cggttagtgt aaacaacgca aaccatacca cacctagttc cgataaagaa ggagcattta
     3241 cattttcgta acctgtaatt ggcgtgtaac gttttatcag ccaagggcgt gcataagcaa
     3301 taaacacaca agctgcagca tgcatacctg ctgtgttatt gaacatatca atagaaaatc
     3361 caaccacaaa tgccaatggt agtatcaacc agcgcggagt ttcaaatggc agcaatatga
     3421 tgaacatcgg atatacatac ggatgtatat aggtgcttac attgagctta ttcagcacaa
     3481 gcacctggaa caaaatcaaa aagattagcc ccacaagcct caatatccaa ttaattgcct
     3541 gcatccattg cgctgttttc taaatcggtt ctttcttttt gtaataattc gtttaccaca
     3601 tatacaaatt gcagcgaact gaatttagtc gataaacgaa cattaagtgt gtaataatta
     3661 cttcccgggt ctttcccaaa actttcaacg gttcccacca taatatcagg tggaaacata
     3721 ggcgaaattt tagtagtcac cacactatct cctttattta atgttcccgg ttctgaaacc
     3781 tgaataacac taatatgtgt cggcgatttc ccttcccagg ttaaacgacc ttgggaatta
     3841 tttttttgga cagccacacg gctaacaaac tgactatgta atacactcat tactacactg
     3901 aatttgggcg acacttttac cacacgtcca acaattccgc ttgaagtaat tacacccata
     3961 ttttcgcgta taccatcatt acttcctttg tttagggtga tgtaattatt aggctcagtg
     4021 aatgaattac caattacctc agcaggtata taagaatata ggggttggcc caaactgtct
     4081 tttacccaaa cagatgaaga atcgggtaag gcacttaaca acaattgctc acgcaaaagg
     4141 gcattttcat gcattaaact atcattcact tctttcagcg aaaaatactc ttgaaaattg
     4201 ccataggtat ttaatacatt tcctgaaaca ccgtttgccg aattaataaa aaaagtattg
     4261 tgaaaattat tgtaggtaaa tacaaaatac aacgatatta attcgagtaa accgaaaaga
     4321 aaaacggcat agtgacggat gatgaataaa aagaaactac gcattattca tccggggaat
     4381 taactcatta gcaatgctgc atattgttca cgatttttca atgcaatacc ggttccacga
     4441 acaactgctt taagtgggtc atcagcaacg tgcaccatca gttttgtttt ctgactgata
     4501 cgtttatcga gaccacgcaa taaagcgccg ccgcctgtga ggtaaattcc attgcgttgg
     4561 atatcacttg ccaactcggg aggagttgtt tcaagtgctt tgagaatagc ctcttcaatt
     4621 tttgaaatgg acttatcgag agaatgtgca atttcggagt aggtaactac tagttgttta
     4681 ggtatacctg tcattaggtc acgaccactc acctggtaat cttcaggggc ttcatctaac
     4741 tcagttaaag ctgagcctac atttattttg atggcttccg ctgttctttc accaatcagc
     4801 aagttgtgct gacgacgcat ataatccact atatcagctg taaattcatc acccgcaacg
     4861 cgtattgact ggtcgcaaac aatacctgaa agtgctacga cagcaatttc tgttgttcca
     4921 cctccaatat caataatcat attaccgaca ggctcctgta catctaaacc aatacctaac
     4981 gctgccgcca ttggctcatg aatcagataa gcttctcgtg ctcctgcttg ttcagcacta
     5041 tcttttactg ctcgtttttc aacttctgta atgcctgaag ggatacaaat aaccatcacc
     5101 aacgacggtt tgaacaactt gtttttgggg aaaatcatac ttatcatccc gcgaatcatc
     5161 gattctgcag cctgaaagtc agcaataaca ccatctcgca acggtcgaat cgttttgata
     5221 ttctcatggg ttttttcgtg catttgcatg gcgcgtttgc caacagcaat cactttttct
     5281 gttttacggt caatagctac aatggatggc tcgtcaacca tcactttatc atgtgctatg
     5341 ataagcgtgt ttgccgtacc gagatcgatt gctatttcct gagtgagaaa gttaaaaata
     5401 cccatggttg tttcttaaat gtacctaaag gtgcaaaatt aaagtttgtt aaggatatgc
     5461 gcgggaaaaa tcaataaatt gttatacaaa cttcaatatt ttttttcaat actaaaaata
     5521 ctaagcgcat tattttaata attatttttt tacacgaaca ttttaagttt ttgtaagtga
     5581 taatgtaaac aattattgca gcatataact cattattgtt tgggtatatt tcatttcaac
     5641 ttgtaataat tttacaagac aaaaaaaccg gcacttgctg gttaccggtt tatcaatttt
     5701 attgctcgtt atatcaatgt ttaaagtgtc gggtaccact caataccatg gccatttgat
     5761 gttcattaca atagtccacc gagtccttat cccgaatact gcctcccggc tgaataacgg
     5821 ctgaaatgcc cgcctcatgg gctatttgaa cgcaatcagg gaaagggaaa aacgcctcac
     5881 tagccatcac cgagccactc acatcgaaac caaaactgcg cgctttatta atggcctgat
     5941 taagagcatc tacccttgaa gtttgtcctg taccacatcc tatcatttga ccatttttta
     6001 ccaatgcaat accatttgat ttcaaatgtt taacagcaat ttctgcaaac aataagtctt
     6061 gcaattgtgc atctgttggt ctaacctcag taacatactt taactcttgc aaagctgttg
     6121 ccttgttgtt gcggtcctga acaagcacac cattcaacat actcttatat tgcttagccg
     6181 gaaattcaaa cattttttgt ctgagtataa ttctgttctt tttagtttgt aacaccacca
     6241 atgcatcatg ttcaaatgca ggtgcgatta aaacttcaaa aaacaattca tttattttat
     6301 aagccgtagc taaatcgata atttgattgc agataagcac gcctccgaaa gcagataccg
     6361 gatcagccgc caatgccttg tcccacgctt cttctgtggt tttagctgta gttaagccgc
     6421 atgcattagt atgcttaata atagcaaatg tgctgaggcc tttttttgct gtttctgcaa
     6481 attcttctat caaatacacg caactttcaa tatcaaccaa gttgttataa gatatttctt
     6541 tgccatgtaa tttctcaaac acatcagtca gtttaccata aaaagctgca tcctgatgtg
     6601 ggttttctcc ataacgcatg gctattttcg gggtataacg cacaccataa ttttcgttgt
     6661 gattaaacca cgtatagatt gcatcatcat aatgcgaggt aatttcgaaa gcgcgtttag
     6721 caaaatattt gcgctgtccg aaataggttt caccattttt ttcgttgagc atttctaaca
     6781 atggtgcata atcatctttt gatgcaataa ttaaaacatc attacagttt ttagctgctg
     6841 cacgtattaa cgatacacct ccaatgtcaa ttttctcaat aatatctgct tcaaccgtag
     6901 tgcttttcac agtttcttca aaagggtata aatcgactat aaccaaatct atttccggga
     6961 tttcatattg agccagctga gccatgtctt ctttgagctc acgacgggct aaaattcctc
     7021 caaaaacctt tgggtgtaag gttttaaccc tgccaccaag aatgctcgga taggtagtta
     7081 aatcctccac agctaatacc ggtacaccta atttttcaat aaattcacgt gtgccaccgg
     7141 tggaatataa tactactccc aacgcatgca atttttgaat aactggttca agttggtctt
     7201 tataaaaaac agaaattaag gctgatttaa tcttaattgg agcgtgcata ctataataat
     7261 ttattaagcc gcaaagttac ccttaagcaa tcaatacttg aagtaaagcc aggatgatat
     7321 tcatcagaaa tgcgcttaaa tattatttag caacagtttt tccactatgc cgggtaaata
     7381 ggcatattcc aattctctta cccttctttc aatactttca ggggtgtcgg ttggggagat
     7441 gtcaatactt atttgaaata aaattcttcc ttcgtcatat acttcatcta cttcgtgaac
     7501 ggtaattcct gtttcatcaa cgcctttgtc aaaaacagcc tgatgtacgt ttttgccata
     7561 catgcccttc cccccaaaat caggtaaaag tgctggaccg atcggc
//
LOCUS       DAORKW010000123         7589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_295_length_7589_cov_4.135386, whole genome shotgun sequence.
ACCESSION   DAORKW010000123 DAORKW010000000
VERSION     DAORKW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_295_length_7589_cov_4.135386"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLB46_06550"
     CDS             complement(<1..630)
                     /locus_tag="PLB46_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76212.1"
                     /translation="MGSVQGLAIKWLLPELNNSIESWCFKVSGCTEEDLVFYDIVIKY
                     YNRILDCINATSEGDSIIFVGWGFTPRLDLKSGFSDLDSKDKDFVFQKANTLETVILQ
                     AAKRNVNVNILLTSDHVWCNTSAVIKNFRNNKNIKTYIDNQLYEFHQKGCFFFTKGTP
                     ILFLGGMDLHPKANDHIDVQLEIRGTSALLGAGTFYERINSLENGSLQSM"
     gene            complement(653..3625)
                     /locus_tag="PLB46_06555"
     CDS             complement(653..3625)
                     /locus_tag="PLB46_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76213.1"
                     /translation="MNKLSDLTKYRGTLPYASEMFGVYTPLLGWKSARKVKKMQMAFQ
                     TAKSQFLNALALKYKPSVKVVFNNGFLEPDHFEPATISHNKLAIQHESLLLQNIALML
                     EGKDILSASDWKDLLKKEALAGMLNGNSNNTVFKQIQNLHNNFINNLDNIPSNVNESQ
                     EALAARKQQLKNQKVIETKNELEKESVIAGMLADLASNEMTLQLEQIFYVNKEGDVSK
                     EIKQLQEALKDEMDDPFLNFDPKKDIKNVSVSPIGIVNLYRQFFFELDTFLGSPVGHV
                     WISPGATVELIEVSTRRTIIEKSFESSFESSNKQESSSTTQDDLSNATKQDNKEDTKT
                     GFSTTVNTSTPIVSISATASLNMANSQQLSREQTQKQMRQQSNKLSSEIRQNYKSTFK
                     TITETTDTSSKRYILTNPSDEKLINYEMRRKMRKVGVQVQDMGSYLCWETFVDEPGEN
                     LGLANLVHIAKPADLVPPPDQTMLPELPESKDYVFTEPLNWKGGDRSVPWEESTGVIW
                     GPTCRISFPDGYEVDENSNAISQVKPCYGVDSNLTHWAFKFKIDPGLQTFSIGIWVDE
                     KYRAIQKIPTLAGYIEYKNQGLNVDEQHDFKLTGVIKLKLTTAKRQSIVDANNAKIKA
                     GYATDKENQRRTQEAFIQAAKDRIKLASNIKQRNFEDLREEERTIVYRKLIAELMTES
                     NYNLPDTPENYEPRHVFSELINAVFDIDKMLYFVAPEWWKPRKHFNQAVLPKMNNGLQ
                     KGPFKLVNASINDSFKTAMVMPFKKDFTVPKELRGSITNWSEGENREDNYFITEDSEP
                     AKLGSSLGWLLQLDGDKHRNAFLNAPWVKAIIPIRPGKEEAALNWLQNAGVEGVDGLN
                     AIYSQQNEYPFIIQKLNENGLVANNPPTISDAIKALCIEVKEKNKASMSVDTFPKDPD
                     INDDQRISATPIEKVFEHGFYPLQGGFRANPNPDGNEDPNNKEKYFNIFTQWLEILPT
                     DQIVPVPVEYDAVTGTMK"
     gene            complement(4200..4496)
                     /locus_tag="PLB46_06560"
     CDS             complement(4200..4496)
                     /locus_tag="PLB46_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76214.1"
                     /translation="MLADSISQWWEINQATWTRFSALKTALASTNKRSQVLALEYLRF
                     DNTIREGLILESYKTELEPLVKAIKNSNNSNADQSKYLLEDKEFYWLKVRKNTH"
     gene            complement(4509..5195)
                     /locus_tag="PLB46_06565"
     CDS             complement(4509..5195)
                     /locus_tag="PLB46_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76215.1"
                     /translation="MRKKYSNQLFHLVKNLGIEPNNLEMEDITDGVFIVKVIKYIGTP
                     LSFFIREETGSFDLFDYKYNQFKPGFPQTLYYPYDKYVSFNEVLDAFKFWINSHVMEY
                     LFEENEPDLWNEFKLGNKSLNIEAIDFSNRDNFSADERKQIVMSINELRFLIHNEISP
                     NEEEKLLIKERLEYLIESTNRLNKYDWKGLVITTLIGIATTLSLDTEKGILLFELFKR
                     VFNNLPKLLP"
     gene            complement(5854..6540)
                     /locus_tag="PLB46_06570"
     CDS             complement(5854..6540)
                     /locus_tag="PLB46_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76216.1"
                     /translation="MSNLKNIQRRISEGVSGWLLFEFNCLRGTLFNEKYLSYPVGQIL
                     NSITDYKTLTEINHPCSNNGQGRPLQVDFVLQDKNSKWKYAFESKWIGNSTISLGFVI
                     WDIIRLQNIYKHHPDIRCYFILAGFDKKINILLEDFDLYYNKDTIKKNEIAKANSTFL
                     IFDIFKLDSSTKTYLNKKIKKYPNFNVYSKIRCRPAHKFPKKDVINMTFSTYIFEVLK
                     PDETHKLRQV"
     gene            complement(6600..6693)
                     /locus_tag="PLB46_06575"
     tRNA            complement(6600..6693)
                     /locus_tag="PLB46_06575"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
BASE COUNT         2322 a         1379 c         1138 g         2750 t
ORIGIN      
        1 catactttgc aggcttccgt tttcaagcga attaattctt tcataaaatg tacctgcacc
       61 taaaagtgct gaagttcctc tgatttctaa ctgaacatca atatggtcat tagcctttgg
      121 atgtaaatcc attcctccta aaaataatat tggtgttcct ttggtaaaaa agaaacaccc
      181 tttctggtgg aactcgtata attggttgtc aatatatgtt tttatatttt tattattcct
      241 aaaattttta ataactgcag aagtattaca ccatacatgg tctgaagtca gcaaaatatt
      301 cacgtttaca ttcctttttg ctgcctgcag aataactgtt tcaagtgtgt tggctttttg
      361 gaaaacaaaa tccttatctt tactatcaag gtcagaaaaa ccacttttaa gatctaaacg
      421 aggagtaaaa ccccaaccta caaatataat ggaatctccc tcgctcgttg cattaatgca
      481 atcaagtatt ctattatagt actttattac aatatcataa aatactaaat cctcttcagt
      541 gcatcctgac accttaaagc accatgattc aattgaatta ttcaattctg gcaaaagcca
      601 tttaattgct agtccttgta cagaccccat attctacgat ttttttatcc ccttatttca
      661 ttgtccctgt tacagcatca tactctacag gaactggcac gatctgatcg gttggcaaaa
      721 tttccaacca ttgtgtaaaa atgttaaaat atttctcttt attatttgga tcttcattac
      781 catccgggtt ggggtttgcc ctaaaacctc cctgcaatgg ataaaaacca tgttcaaaaa
      841 ctttctcaat cggcgttgca gatatccgtt gatcatcatt tatatcaggg tctttgggaa
      901 atgtatcaac actcattgat gccttatttt tttcttttac ctcaatgcat aatgctttta
      961 ttgcatcaga aattgttggt gggttatttg caacaagacc attttcattt aacttttgta
     1021 ttatgaatgg atattcattt tgctggctat aaatggcgtt aagcccatca acaccttcaa
     1081 caccagcatt ctgcagccaa ttcaaggccg cttcttcttt tcctggtcga atcggtatta
     1141 tagcttttac ccaaggcgca tttaaaaagg cattgcggtg cttatcgcca tctaactgta
     1201 gcaaccatcc aagagaacta cctaactttg ccggttcgga atcttctgtt ataaaatagt
     1261 tgtcctctct gttttcacct tcgctccagt tagtaatact ccccctcagt tccttaggca
     1321 cggtaaaatc ttttttaaat ggcattacca tagctgtttt gaatgaatca ttaatagatg
     1381 cattcacaag tttaaaagga cctttttgca aaccattatt cattttgggc aaaactgcct
     1441 ggttaaaatg cttgcgtggc ttccaccatt caggtgctac aaaatagagc attttgtcaa
     1501 tgtcaaatac tgcatttatc agttctgaaa atacatgacg gggttcataa ttttcaggtg
     1561 tatctggcaa gttataattg ctttctgtca ttaactcagc aattaacttt cgatacacaa
     1621 ttgttcgttc ttcttccctc aaatcttcaa aatttctttg tttgatatta cttgccagtt
     1681 taatcctatc ttttgcagcc tgtataaatg cctcctgtgt tcgacgctgg ttttctttat
     1741 cggtagcgta acctgcttta atttttgcat tatttgcatc aacaatgctt tgccgtttgg
     1801 cagtggttaa tttaagttta ataaccccgg taagtttaaa atcatgctgc tcatcgacat
     1861 ttaagccttg gtttttatat tcaatatatc cggccagtgt tgggattttt tgtatagctc
     1921 tgtatttttc gtcaacccaa attccaatac taaatgtttg caaacctggg tcaatcttaa
     1981 atttaaatgc ccaatgtgtc aggttactgt caacaccata acaaggtttt acctgtgaaa
     2041 tagcattact gttttcatca acttcatatc catcaggaaa acttattctg caagtgggcc
     2101 cccagataac cccagttgac tcttcccacg gtacagatct gtctccacct ttccaattta
     2161 atggctctgt aaatacataa tcttttgatt ccggaagttc aggtaacata gtttgatcag
     2221 gaggcggcac tagatctgca ggttttgcaa tatgcacgag gttcgcaagc cccaagtttt
     2281 cgccaggttc gtctacaaac gtttcccaac aaaggtaact gcccatgtct tgcacttgga
     2341 cacccacctt tcgcatttta cgccgcattt cataatttat taatttttca tctgatggat
     2401 ttgttaatat ataccgtttg ctcgatgtat cggttgtttc agtaatagtt ttaaaagttg
     2461 atttataatt ctgcctaatt tctgacgata gtttattaga ttgctgccgc atttgctttt
     2521 gtgtttgctc tctgcttaac tgctggctat tggccatatt taaactagca gtggcagaaa
     2581 tactaactat tggagttgaa gtgtttacag ttgttgaaaa acctgttttt gtatcctctt
     2641 tattgtcctg tttagtcgcg ttgcttaaat catcctgtgt tgttgaactc gactcctgct
     2701 tattcgagga ctcaaatgaa ctttcaaacg atttttcaat aattgttctg cgtgtgctca
     2761 cttcaatcaa ttctacagtc gcaccgggtg aaatccatac atgccccaca ggagagccta
     2821 aaaaagtatc aagttcaaaa aagaactgac gatataaatt cacaattcct ataggcgaga
     2881 ctgacacatt tttaatatcc ttcttaggat caaaattcag aaatgggtcg tccatttcat
     2941 ctttcaatgc ttcttgcaat tgcttaattt ctttgctcac atcgccttct ttgtttacat
     3001 aaaatatctg ttcaagttgt aaagtcattt catttgatgc caaatctgcc agcatacctg
     3061 ctattactga ttctttttct aattcattct ttgtttcaat gactttttga ttttttaatt
     3121 gttgcttcct agctgcaagt gcctcttgtg attcattaac atttgaagga atattatcca
     3181 aattgtttat aaaattgttg tgtagattct gtatttgctt aaaaacagtg ttgttgctat
     3241 taccattaag catgcctgca agcgcttctt tttttaataa atctttccaa tcagaagcag
     3301 aaagaatgtc cttgccttca agcattaagg caatattttg tagtaaaaga ctttcgtgtt
     3361 gtatagccaa tttgttatgc gaaatcgtag ctggttcaaa atggtctggc tccaaaaacc
     3421 cgttattaaa tacaacttta acagagggct tgtatttgag agctagcgca tttaaaaatt
     3481 gactttttgc tgtctgaaac gccatttgca ttttttttac ctttctcgca gatttccagc
     3541 ccaacaaagg agtatatact ccaaacattt cacttgcata aggcaaagtg cctctgtatt
     3601 ttgttaaatc gcttaatttg ttcattatta atatagtttt ttgttaaaat taatagtaat
     3661 tagagtcaat actaaaagta ggttaattcg catttttttt taatagatat taacctattt
     3721 catttgagaa attttcatct attaaacttc ttaaagcaca ttttattggg agatttaaag
     3781 tagagttaaa tttcaaattt gtttaatatt ataatgtcca attcaaagtt attagaatga
     3841 attggcattg tgtcttttat tccttcattt tatttttttt atttgtattt aaaattgaaa
     3901 atcacgcgaa taaatggtcg gtgcactttt ataaaaaaat aatttcaata acccatttca
     3961 aatttacgta attaggatac ctaaagtaaa tcccaacccc aaattcacgg tttaccgtta
     4021 atctgaaata tgtcacatgc attttcataa aacataacat atttgatact ttcagcccac
     4081 taattcattt ttttgcacat ttaaccatgt cacaaaaaaa attattttcg caaaatttta
     4141 ggggcaattt aaaatggggt taaataggat attaacgccc tcctaccgca aaaacgttgt
     4201 tagtgcgtat ttttcctcac cttcaaccaa taaaattcct tatcctcgag caaatattta
     4261 gactgatctg cgttggaatt gttactgttt ttaattgctt taaccaaggg ttccagttca
     4321 gttttatagg attcaagtat tagcccctca cgtattgtat tatcaaaacg taagtattct
     4381 aatgctaata cctggcttct tttgttggtg cttgcgaggg ctgttttgag tgcggagaag
     4441 cgtgtccagg tggcttggtt tatttcccac cattgtgaaa tggagtcagc aagcatttgt
     4501 ctgtcagatt aaggtaaaag cttcggtaaa ttattgaata cacgcttaaa caattcaaaa
     4561 agaagtatgc ccttttcagt gtcaagtgag agggtagttg caattccaat cagtgtggta
     4621 attaccagtc ccttccaatc atatttattg agcctgttgg ttgattcaat taggtattct
     4681 aacctttctt ttatgagcaa tttttcttct tcattaggcg aaatttcatt atgaattaaa
     4741 aatcttaatt cgtttataga cattactatt tgcttccttt catcagcaga aaaattatca
     4801 cgattactaa agtctattgc ctcaatattc aaacttttat ttcctaattt aaattcattc
     4861 cataaatctg gttcattttc ttcaaataga tattccataa catgactatt gatccaaaac
     4921 ttaaaggcat ccaaaacttc attaaaagaa acatatttat cataaggata atataatgtt
     4981 tgtggaaaac ctggcttaaa ttgattatat ttataatcaa ataaatcaaa actacctgtt
     5041 tcttctctta taaaaaatga taatggcgta ccaatatatt taatcacttt aacaataaag
     5101 acaccatcag ttatatcttc catttccaaa ttattaggtt caatacctaa attttttacc
     5161 aagtgaaaaa gttggttgct gtactttttt ctcattttta taatgataag attgggggta
     5221 attaatttgg caccaaaaat acaattgatt ttttaagttt caaattgaat ttaacctttt
     5281 taaactgaaa acagtggtaa tttactattt ttaaattttt gccaaacaat cacattaaaa
     5341 tgaattaatt gccaaattta ggcaaaactc aaaagcaata ttctgcgatt tttaacctag
     5401 aaaaccttca tattgcttga atctcccgct taaaattcaa atttccgaac ctatttaaaa
     5461 tgtcacattc tatagcgcaa aaattcttta attaataata ccccggcacc ttctagttta
     5521 taaatctagt accaagccta aaaagcgcat tttttttttg caaaatcatg ctaatgcaag
     5581 taaatataca gcagagtata tttcacataa tactatgaaa aataaggttg gcaaatgttt
     5641 tactttatag tattcccatc aaggttttga attattatct cagaaataat gcttttaact
     5701 ttttaatacg taaaactatt aattgccttg taaagtaaaa tttgaaaatg aatcgaagtt
     5761 tgtaattgta atattgaaat gaccataatt taaagtagac attgtcagat tcaatacatt
     5821 acagaaagac ataaaatcat aagaattaaa atcttatact tgtctgagtt tatgtgtttc
     5881 atctggcttt aaaacctcaa aaatataagt agaaaaagtc atatttatta catctttttt
     5941 cgggaatttg tgagctggtc tgcaccgaat ttttgagtat acattaaagt tagggtattt
     6001 ttttattttt ttatttaaat atgttttcgt agagctatca agcttgaaaa tgtcaaaaat
     6061 taaaaatgtc gaatttgctt tagctatttc atttttcttg attgtatcct tgttataata
     6121 taagtcaaaa tcttcaagta gaatatttat ttttttgtca aaacctgcaa gaataaaata
     6181 acatcttatg tctggatgat gcttgtaaat attttgtagt cgaattatgt cccaaattac
     6241 gaaacctaaa gaaattgttg aatttccaat ccattttgat tcaaatgcat atttccattt
     6301 tgaattttta tcttgtaata cgaagtcaac ttgaagtggt cgtccttgcc cattgttact
     6361 acaaggatga ttgatttcag ttaacgtttt ataatcagta atactattca aaatttgccc
     6421 aacagggtag gataaatatt tctcattgaa tagggtgccc ctcaaacaat tgaactcaaa
     6481 taataaccaa cctgaaacgc cttcgctaat ccgtctttgt atgtttttta agttgctcat
     6541 atactaaacc aaaaaaaaga ccgacccgta taggaccagt ctataacaag gagctttaat
     6601 gtctcaaata ggactcgaac ctacatatga tgggttaaaa tgtttgctcc ttgtttaaat
     6661 gccgccatgt tttaccattt aaactattga gacatataaa attatttttc acaaatatat
     6721 tgattatttg ttgcattatt ttttaacaat cgtttaaatt tattcactga cttctttact
     6781 taccgaaact tcattatcaa tccatgcatt tacactacaa tatggacttt tagacatttg
     6841 tttatcaaat ataataaaaa ttttcctaaa taaaatatat aatgaaccta tctcctaccc
     6901 aaaattttaa aaatccagat atgttattta aaaccacgca aataattaaa atctactctt
     6961 ttgttctagt aaccaataaa tttcaataat accccaccac ctatttcccc ggaactccca
     7021 ctttctccct ccccacaacc tgcaccaaaa caaccttacc ctccgcataa cccgcaagca
     7081 acaaagggga taaaacctca tcaatttgtt tgtagatact tgtgttgcca tcgcggtcga
     7141 ctaataggat tgttatttta tccggttggc cgattattat gaagaggctg tcaggggaag
     7201 ccagggcaat gaagatacgg tctgtggatg tgggttcgag gggaagtcag atattgtatt
     7261 tcttgttttc acaataataa tcggtggaga gatggtaggt gggtttgttg cgggactaaa
     7321 gttcttcata ttggaggtta taggaatcag ggccgtctgc caactgtcga attttggagc
     7381 cttcagccaa attaatattg aagattggtg ccttttctaa ttcgatggta gattaaaaca
     7441 tcctagaata aggattattt tattggtttt tgattcttta atttgtaaca ggctttcatt
     7501 tatttcgccg tcaatattgt atttctcttc gctgacaaat tgggaataga cagtttgtcc
     7561 gacaaaatag agaatattaa aaagattat
//
LOCUS       DAORKW010000124         7515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_304_length_7515_cov_4.238204, whole genome shotgun sequence.
ACCESSION   DAORKW010000124 DAORKW010000000
VERSION     DAORKW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_304_length_7515_cov_4.238204"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLB46_06580"
     CDS             complement(<1..185)
                     /locus_tag="PLB46_06580"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HRF76217.1"
                     /translation="MAYELSGKLLEIFPTQEVSASFKKREFVIEKSESVNDRVFTDVI
                     KFQLIQDRCALLDSYKLG"
     gene            349..726
                     /locus_tag="PLB46_06585"
     CDS             349..726
                     /locus_tag="PLB46_06585"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76218.1"
                     /translation="MMTFLLNIMLMSSTSQAAVMPYAELPGQCIQLPGDLVEVVYYYP
                     NGKIKQVGFLENGNKTGEWVSYNLEGQIVVKAYFENNLKEGKWKVYNDEGELQYKIQY
                     RNGKKVWAQEYDESGNTTAFNYK"
     gene            824..1696
                     /locus_tag="PLB46_06590"
     CDS             824..1696
                     /locus_tag="PLB46_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76219.1"
                     /translation="MRLILGILTLCIVQTAAAQLPAVDIYHGKLTSADGKWALNDLKN
                     ITNRAGYDNQPSFSPDGNTIYFTAMHDTLQADIYTYNLLTAETKQITNTLESEYSPVV
                     MSNGKTFSVVRVELDTVQRMCMFKLNGTKPKVLMNNIDSVGYYCPVDEKHYAFFMVTD
                     TPTLVLADIKKQTTKLIDVNVGRCIKMIPGEQALSYIVKNSDNEFMIKRFDLTTQTTS
                     VITAIPGISEDFIWTKNGELLMARDNQIWMYSYGAEKKQWARILTVKELKGKKIYRLA
                     LAPDGINFAFVGDE"
     gene            1689..2270
                     /locus_tag="PLB46_06595"
     CDS             1689..2270
                     /locus_tag="PLB46_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76220.1"
                     /translation="MNNNLPELPFDFPLTASDLATKAMRAASCKTFADACRYVALLPY
                     RRNQDKKNMLCVAEEKCGTCSTKHTLLKKVAEEHDKDDIHLVIGIYFLNQHNTPKAKP
                     ILNSFYLRGFPEAHCYLTFDSKIYDFTFPSGNFNFAHDLLEEHVVTADEVATKKSGLH
                     KEYLRKWAKQEQLPFTHEKLWDIRELCIAALSL"
     gene            complement(2327..3565)
                     /locus_tag="PLB46_06600"
     CDS             complement(2327..3565)
                     /locus_tag="PLB46_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76221.1"
                     /translation="MKKHLLLISLAILLIAGKQTNAQGICDPSGNILIYSNYDGGSFT
                     INIDENIPNIRIGLCSYEQLNVTITGTYADNVVEVLYAGYNSGGGTSVSGVDAGIVEI
                     LNYPPVTLFDADGYGYMICAYECDTDYVPGGCNTVDQAVDYFSTYLDGNIRYSFFQYG
                     VFSGTENMSDGGNCCLGAEPCAIVVDAGQDASICVGDSVMLEGSGAVNYVWFPDIALS
                     DDGIYNPYSTPETTITYILTGSDADGCSGLDSVTVFVNPYPVVEIMDMGDGNLMATGG
                     GSYQWYLNGEIIPGAINETYVATTTGYYAVEVTSDAGCTTITEEIIVQVDQIANHYLN
                     QQIICYPNPVNNELHITTSTGLQIEQVEIYAINGQMVLSIADLQMTSNIISVEKIPAG
                     LYTIKVLTNEGIAVKQIVVE"
     gene            3813..5234
                     /locus_tag="PLB46_06605"
     CDS             3813..5234
                     /locus_tag="PLB46_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76222.1"
                     /translation="MKTNTHIYKVLLTLLFSVIFFVPDAEAHVKWFTDGSFADRPLQL
                     GEVMEGRFFYTLFTICLITVVFGVWLDSKISKSPWYLKIDGWLEGRKNYATIVLRVAA
                     AMVLILSWQSNAMIVPTIPIPKQFEWIGWFQFVLAFCLLIPRLVPIGGLGIILIWVVG
                     NITLHPFHMLDYLMFAGVGFYLASTSIKQGKFKEFGLVVLYITVGFSLCWVALEKFVY
                     PNWSLFLIKEHPKLAMGLNFDFFLKSIGFVEFGLGFLLLVCLLQRPLAIIITIVFFIT
                     SSIFGKVEIIGHTMIHGALIVFLLEGPGKYYLQLRDRFKNIFKRMAFTAVNFILLFSV
                     LAIGYSYLAQGKYDKKQALLAKNPENLKGQIELAGYPKDEIPSVWMDIKPDMKEGYNI
                     HFITTNFKFAPENVSTMHVMGQGTINLYVNGERIARVYDEWYHVNLKPGINEIMATLN
                     SNELTEYTIRGKPISSSESITVD"
     gene            5301..6026
                     /locus_tag="PLB46_06610"
     CDS             5301..6026
                     /locus_tag="PLB46_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRF76223.1"
                     /translation="MTEVDIFIPCFIDQLFPETGFNMVKVLEKAGCKVHYNPNQTCCG
                     QPAFNAGYWKEMDDVGKKFLADFANSDRYVVGPSGSCVGFVRNFYGTLYANTPVHNYY
                     KGLRKNLFEFSEFMVDVLKVDNLGGKLKAKITYHDACGALRECKIKEAPRKLLNNIEG
                     LEIVEMVDSETCCGFGGTFAVKFEPISTGMADSKVNNAIATGAEYIVSTDYSCLMHLQ
                     AYIDKQQKPIKVLHIADLLAMSL"
     gene            6070..6735
                     /locus_tag="PLB46_06615"
     CDS             6070..6735
                     /locus_tag="PLB46_06615"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HRF76224.1"
                     /translation="MAGIEDITKQIQDYQSRGLKLFTSSSFQTHSTVLLHIISRIDNT
                     IPVYFLNTGFHFPETMMYKQQITNLFNLNTIDVKSNTPKLMQVDENGRFLFTSDPDYC
                     CYINKIQPLEPALNTFDVWINGVRADQNANRATFHQEEKTNHKAFRYHPMLNWTNQMI
                     YAYIREHQIPKHPLDEKGYMSIGCEPCTRQISLEVLADERQARWYGLNKTECGLHTEL
                     IKQ"
     gene            6732..>7515
                     /locus_tag="PLB46_06620"
     CDS             6732..>7515
                     /locus_tag="PLB46_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF76225.1"
                     /translation="MKILITGGAGYIGFELAFALANNVDVESVVLYDNLSRHNENIFF
                     GNAIGNGKLKLIKADILDARKLNAAFKNVDVVYHCAAKVLTPFANQDPHFFEQINHWG
                     TSEIIEAAEKNNIQHLIYLSSASVYDRIAEPATEQTIPNPNTYYGISKLRGEEHIQRL
                     NGMALTHIIRCGNVYGYSNSMRFDAVINNFMLQANFENKISIHGTGAQTRAFIHISNT
                     VEALAQLPFKNVPAGIYNLVTKNLAVNDIAETITTLYPTLERL"
BASE COUNT         2435 a         1315 c         1395 g         2370 t
ORIGIN      
        1 ccaagcttgt agctgtccaa caaagcgcaa cgatcctgaa tcaactgaaa tttgattaca
       61 tccgtaaata ccctgtcgtt tacactttcg cttttttcaa taacaaattc tctctttttg
      121 aaagatgctg aaacttcctg agtaggaaat atttcaagca atttacctga taattcgtaa
      181 gccatttcgc aatttttttc aaagataaaa gtttatttca attccttgaa atttcggcta
      241 tgttaactta aggtaaatcc ggtcaaatgc taatgtaaat ttaatatgaa caaattgcta
      301 atatcaggtt aattacttct taaatttgtg ttaataaaaa tcgaagctat gatgactttt
      361 ctcttaaata tcatgttgat gtcttcaacc tctcaggctg ctgttatgcc ttacgctgaa
      421 cttcccggtc aatgtattca gttacccggc gatttagttg aggtggtata ttattatcca
      481 aatggcaaga ttaaacaagt aggttttttg gaaaatggca acaaaacggg agagtgggta
      541 agttataatt tggaaggtca aatagtagtt aaagcctact tcgaaaacaa cctcaaagaa
      601 ggcaaatgga aagtgtataa cgatgagggc gagttgcaat ataaaattca atatcgcaat
      661 ggtaaaaaag tatgggcaca ggaatatgat gaatcgggca atactactgc ctttaattac
      721 aaataaaatg gttgaatatt attgacttaa taccagttaa gttgattgca ggtatccgcc
      781 tttggcggat atttttttta catttgttca aaataactgc attatgcgat tgattttagg
      841 gattttgacc ttatgtattg tacaaacagc agctgctcaa ttacctgcag tagatattta
      901 tcatggtaaa ttgacttcag cagatggcaa atgggcactt aacgatttga aaaacattac
      961 caacagagcc ggatatgata atcagccatc tttttctcct gatggtaata cgatttattt
     1021 tactgctatg cacgacacct tgcaggcgga tatttatact tataacttat taactgctga
     1081 aacaaaacaa attaccaata cacttgaaag tgaatactca cctgttgtta tgtcaaacgg
     1141 taaaacattt tcagtagtga gggttgaatt ggatactgtg caacgcatgt gtatgtttaa
     1201 attaaatggc acaaaaccta aggtgctaat gaacaacatc gacagcgttg gttattattg
     1261 ccctgttgat gaaaaacact atgcattttt tatggtaacc gatacaccta cactggtatt
     1321 ggcggacata aaaaaacaaa ccactaagtt gatagatgta aatgttggcc gatgtattaa
     1381 aatgattccc ggcgaacagg ccttgagtta tattgtaaaa aattcagata atgaattcat
     1441 gataaaacgg tttgatttaa caacccaaac tacctcagtt ataactgcta ttccgggtat
     1501 tagtgaggat tttatctgga ctaaaaatgg cgagttattg atggctcgcg acaatcaaat
     1561 ttggatgtac agttatggtg cagaaaaaaa acaatgggca agaattttaa ctgtcaaaga
     1621 attaaaaggt aaaaaaatat atcgcctggc attggctcct gatggaatta attttgcatt
     1681 cgtaggtgat gaataataat ttaccggaac tcccttttga ttttccctta actgcttccg
     1741 acttagctac caaagcaatg cgtgctgcca gctgtaagac atttgctgat gcttgtcgtt
     1801 acgttgcttt attaccttat agacgtaatc aggataaaaa aaatatgtta tgtgttgcag
     1861 aagaaaaatg cggcacttgc agtactaaac ataccttact taaaaaagta gcggaagaac
     1921 atgataagga tgacatacat ctggtaattg gtatttattt tttaaatcaa cacaatacac
     1981 caaaagcaaa accgatacta aattcttttt atttgcgcgg atttccggag gcacattgtt
     2041 accttacttt tgattcgaaa atttacgatt tcacattccc ttcaggtaat tttaattttg
     2101 cacatgattt attagaagaa catgtagtaa ctgcagatga agtggcaact aaaaaatcag
     2161 gcctgcataa agaatacctg cgcaaatggg caaaacaaga acaactacca tttacccatg
     2221 aaaagctatg ggatattcgg gaattgtgta ttgcggcact gagtttgtaa ataaaaaaag
     2281 ctgtatgcgt ttaaacatac agctcatatt aaattgaatt atttagttat tccaccacaa
     2341 tttgtttcac agcaattcct tcattagtta atactttaat agtatataaa cctgccggaa
     2401 ttttttctac ggatataata ttggatgtca tttgtaagtc cgcaattgat aaaaccattt
     2461 gaccattaat ggcataaatt tcaacttgtt caatttgtaa accggttgaa gtagtaatat
     2521 gtaattcgtt gtttacagga tttggataac aaattatttg ttgatttaaa taatgattgg
     2581 caatttggtc tacttgtaca ataatttctt cagttattgt tgtgcaacca gcatcgctgg
     2641 ttacttctac agcataataa ccggttgttg ttgcaacata agtctcatta atagctccgg
     2701 gaattatttc accgtttaaa taccattgat aagaaccacc acctgttgcc attaaattac
     2761 catcacccat atccataatt tctacaacag gatacggatt aacaaataca gtaacactat
     2821 ctaaacctga acaaccatca gcatcgctgc cggttaatat ataagtaatt gttgtttccg
     2881 gagtggaata aggattgtaa ataccatcat cacttaaagc gatatcagga aaccacacat
     2941 aattaactgc accggaacct tctaacataa cactgtcgcc aacgcatata gaagcatcct
     3001 gccccgcatc aacaacaatc gcacatggtt cagcaccaag acaacaatta ccaccatcgc
     3061 tcatgttttc tgtcccacta aaaacaccat attgaaaaaa ggaatatcgg atatttccat
     3121 caagataagt gctaaaataa tctactgcct ggtccactgt attacatcca ccgggaacat
     3181 aatcggtgtc acattcataa gcacaaatca tgtaaccata tccatcagca tcaaataagg
     3241 taaccggtgg ataatttaaa atttcaacaa taccggcatc aacaccacta acactggttc
     3301 cgccgccact attataaccg gcatataata cttctaccac attatcggca taggtcccag
     3361 tgatggtaac atttaactgt tcgtatgaac ataatccaat tcggatattg ggaatgtttt
     3421 catcaatgtt aatagtaaag ctgccgccat cataatttga ataaatcaaa atgttgcctg
     3481 aagggtcaca aatgccttga gcatttgttt gtttccccgc tataagcagg attgctaaag
     3541 aaataaggag taggtgtttt ttcatatgtt taaagctttt acatttaaag ttaaagataa
     3601 ataaacagtt attgtagaac aatgcaatag taacgatttc ttcatataag gaaaattatc
     3661 aattaacatg gaaaattgtc attataagga ctgtaactta agtcacaact tagataatat
     3721 ttccctctgt ttacatttct tttacaatta atgataacta cccgttaaca gtgtatttac
     3781 atttgcaaat agcggctagt atgagagaat ttatgaaaac taacacacac atttataagg
     3841 ttctgctaac cctgttgttt tcagttatct tttttgttcc cgacgccgaa gctcacgtca
     3901 aatggtttac tgatgggagt tttgccgatc gaccgctgca actcggcgaa gtaatggagg
     3961 gtcgtttttt ttataccctc tttactattt gccttataac cgtagtattt ggtgtttggc
     4021 tcgatagtaa aatatcgaag tcgccatggt accttaaaat agatggctgg ctcgaaggcc
     4081 ggaaaaacta tgcgaccatc gttttacgcg tagcagctgc tatggtgctt attttaagct
     4141 ggcaaagtaa tgccatgatt gtccctacca tccccattcc caagcagttt gagtggattg
     4201 gctggttcca gtttgttttg gcattttgtc tcttaattcc acgtttggtt ccaattggtg
     4261 gattaggtat tattttgatt tgggttgttg ggaatattac cctgcatcca ttccatatgc
     4321 tcgattatct gatgtttgcc ggggtaggct tttatctggc cagcaccagt ataaaacaag
     4381 gtaaattcaa ggagtttggt ctggtagtgc tctatatcac agtcggtttt tcactctgtt
     4441 gggtagccct tgaaaaattt gtgtatccca actggagttt atttcttata aaagaacacc
     4501 cgaaactggc catgggcttg aatttcgact ttttcctcaa atccattggg tttgttgagt
     4561 tcggattggg tttcttgtta ttggtttgtt tattacagcg cccgctggct atcataataa
     4621 ccatcgtgtt ttttattacg tcctctattt tcggaaaagt ggaaatcatc ggccatacca
     4681 tgattcacgg tgcattaatc gtgtttctgt tggaaggtcc cggcaaatat tacctgcaat
     4741 tacgcgatcg ctttaagaat atttttaaaa gaatggcctt cacggcagtc aattttatac
     4801 ttttattttc tgtgttggca attggttatt cttacctggc tcaaggtaaa tacgataaaa
     4861 aacaagcatt attagccaaa aatccggaaa acctcaaggg gcaaattgaa ctggccggtt
     4921 atccaaaaga tgaaattcca agtgtttgga tggacattaa accggatatg aaagagggtt
     4981 ataatatcca ttttatcacc acaaacttca aatttgctcc tgaaaatgta agtaccatgc
     5041 atgttatggg ccagggcact attaatttgt atgtaaatgg tgaacgcatc gccagggttt
     5101 acgatgagtg gtaccatgtg aacttaaagc cgggtataaa tgaaattatg gcaaccctga
     5161 actctaacga acttacagaa tatacaatta ggggcaaacc tatttcctcc tccgaatcaa
     5221 taaccgtaga ttaatttatt gtgtgtcagg tgttaacaag ctatatgcgc aattaaccta
     5281 cgtttgctac ttttgctttt atgactgaag ttgacatttt cattccctgc tttatcgacc
     5341 aactattccc tgaaacgggg tttaatatgg ttaaagtgct cgaaaaagca ggttgtaaag
     5401 tgcattataa tcccaaccaa acatgttgcg gacaacctgc atttaatgcc ggatattgga
     5461 aagaaatgga cgatgttggg aagaaatttt tagcggattt tgcgaactcc gacagatatg
     5521 ttgtggggcc ttcaggttca tgtgttggat ttgtaaggaa cttttatggc accttatacg
     5581 ctaatactcc tgtgcataat tattataaag ggctgagaaa aaatttgttc gaattcagcg
     5641 aatttatggt tgatgtgctc aaagtggata atctaggtgg taaactcaaa gcgaaaatta
     5701 cctatcacga tgcatgtggc gcattgagag aatgtaaaat aaaagaagcg ccacgtaaat
     5761 tattgaacaa tattgaaggg ttggaaattg ttgaaatggt tgattcggaa acatgttgtg
     5821 gtttcggcgg aacctttgca gtaaaatttg aacctatttc aacagggatg gccgattcaa
     5881 aagtaaataa tgcaattgca acaggcgcag agtatattgt ttctaccgat tattcttgtt
     5941 taatgcactt gcaggcttat atcgataaac aacaaaaacc gattaaggtg ttgcatattg
     6001 cagatttatt ggcaatgagt ttataattat tgttgttgtt tcttgaattg ggattaaaaa
     6061 ttgatgttaa tggcagggat tgaggatata acaaaacaaa ttcaggatta ccaatcccgt
     6121 ggattgaaac tatttacttc ttcttcgttt caaactcata gtaccgtttt attgcacatc
     6181 atttcacgta ttgataatac tattcctgta tattttttaa atacgggttt ccattttccg
     6241 gagaccatga tgtataaaca acaaattacc aatttgttta atctcaatac gattgatgta
     6301 aaatccaata cacctaaact tatgcaggta gatgaaaatg gaaggttttt atttacatct
     6361 gacccagact attgttgtta tatcaacaaa attcaaccac ttgaacctgc attgaatact
     6421 tttgatgtat ggataaatgg ggtacgtgcc gatcaaaacg ccaatcgtgc aacatttcat
     6481 caggaagaaa aaacaaatca caaagcattt cgttatcatc caatgttaaa ttggaccaat
     6541 caaatgattt atgcttatat acgtgaacat caaataccga aacatccgct cgatgaaaaa
     6601 ggatatatga gtattggttg tgaaccctgc acacggcaga tcagtctcga agtgttggca
     6661 gatgaaaggc aggcacgttg gtacggctta aacaaaacgg aatgcggatt acatacagaa
     6721 ttaattaaac aatgaaaatt ttaattaccg gtggtgccgg atatattggt tttgaattag
     6781 cttttgcttt agctaataat gttgatgtgg aaagtgttgt gttatacgat aatttgagtc
     6841 gccacaacga aaatattttt tttggtaatg ccattggcaa tggaaaatta aaactgatta
     6901 aagccgatat tttagatgcc agaaaattaa atgcggcttt taaaaatgtg gatgtggtgt
     6961 accattgcgc agcaaaagta cttacgccat ttgctaatca ggacccgcat ttttttgagc
     7021 aaattaacca ctggggaacc agcgaaataa tagaagctgc agaaaaaaat aatatacaac
     7081 atttaattta tttaagcagt gcatctgttt acgaccggat tgcggagccg gcaactgaac
     7141 aaaccattcc taatccaaat acatattacg gtatttcaaa actgagggga gaagaacata
     7201 ttcaacgatt aaatggaatg gcactcacgc atattattcg ttgtggaaac gtttatggtt
     7261 atagcaattc gatgcgattt gatgctgtta ttaataattt tatgttgcag gcgaattttg
     7321 aaaataaaat ttcaattcac ggcacaggcg ctcaaaccag agcttttatt catatcagta
     7381 atacggtaga agcattagct caattaccat ttaaaaatgt gcctgcgggc atatataatt
     7441 tagtgacaaa aaatttggca gtaaacgata tagctgaaac aattacaaca ttatatccaa
     7501 cattagaacg tttat
//
LOCUS       DAORKW010000125         7471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_308_length_7471_cov_7.726537, whole genome shotgun sequence.
ACCESSION   DAORKW010000125 DAORKW010000000
VERSION     DAORKW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_308_length_7471_cov_7.726537"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLB46_06625"
     CDS             <1..236
                     /locus_tag="PLB46_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76226.1"
                     /translation="FSNWRYGYKIFLNLTPGTLDLTPRGRAKNISPLPNRNIPLSFSN
                     WRYGYKIFLNLTPGTLDLTPRARVKNISPNEFN"
     gene            complement(1004..1729)
                     /locus_tag="PLB46_06630"
     CDS             complement(1004..1729)
                     /locus_tag="PLB46_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF76227.1"
                     /translation="MQGSIVIIEDNPQHVTDLVHLLNKFYPLLNIRAVADSYAAALEA
                     IENHKPDLVFLDIELSGSKTGIDVLKFYHNLNLNVIYTSRLDKGVQALNLGAKYYLTK
                     PFTEDDFIEAIRAWEKRRNNVVTIESKYIYLPELNNPIFRIQISEIIALKGNKDYSIL
                     YSSNTKLNNKTVSKRLNILEDDFNKSGYSNIIRTSKSYLINSDHIEKAEKQFNIIIMS
                     NLLQVPVSFTQAREKIKKIIFLK"
     gene            complement(1729..4197)
                     /locus_tag="PLB46_06635"
     CDS             complement(1729..4197)
                     /locus_tag="PLB46_06635"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF76228.1"
                     /translation="MKTILLVMFAASAFFFSIENSISESNAKNTLTVIDSNHLFNLID
                     TADILSNTDEYGALLLYDSIIQLADLYGYQNPKIYAQINKAQLLLFIDSIDAADELIN
                     KAISNNNFYSDYYYPLKGFIVKAEIQNYLGEFDSALYYLNAAKPFGNDEYYFIGRLYY
                     DLAQLYSDQNNYDSMIQLYSTALEYFKLANDPNRTISTYAKIGEAYKLKGNLDSSYGI
                     LMECDSMINVTPNLFNITIAQAYLDIGDIYKAKSLFKPAIEYYEKCIPLFEREYDFYN
                     LAIVKQRIGNLLMDKGNYVDGKIYLYSMLDICIKYQYNNLIPRVYNSIANIENRSGDY
                     EKALENANAGIAICEQTNNIATTWSLLISKATALSGLGNHDSALQYFIEADVVSERVS
                     VFTAKFLPKYSLAAEYQLTNNLILADSFANIALAIAETAEQQKEISMANNLLYKINLE
                     KGNYKAALENYITGKIYKDSLRNIENNFTIETLNKKYQLAEKDKMLVETNLRNELVER
                     NLRLQSITLLEQQTQNRVITLINDSLQRTAELSNQAFELEKAKVKQAEDQKSLALLKA
                     QSEKQQRELEQQKRAKQKNWFVLFSILFFVIAFSIIILVKQIGKKKVLQIKNNFLSYW
                     MNTHFIANNIALNKGDTRENPERAILRGDLLSSITSDIFHFAKIDKITLREELNIVEK
                     FIKIEGLRLENRFNYSIKIVPENAPLHDIVMKPPLAIQTLVENTIKHAVEASNYIINI
                     EVAINVYESFIKINVIDDGPGFDKDITLESILQNKEPSAIQIIYERLRIECKKKKIKD
                     FIKINHINQGSNIELIIPSSVKLM"
     gene            complement(4211..5725)
                     /locus_tag="PLB46_06640"
     CDS             complement(4211..5725)
                     /locus_tag="PLB46_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76229.1"
                     /translation="MTPLEIREVEFSFKQRGPRALQSDPAKLFRALQSVSAYNTETIG
                     YAWRRQFKEKSKPMESKAFTDARYILKGIILDVLVKSNFKYLGGEAERLLYHAEILSS
                     KGMYEESHKALKQALIETNHCVSPFYKSMILQKLLNITPKVSGKNLEENVSYLLHQIE
                     QDAQDLNQFFLINKLNTHTALTLFKTAILNDGSDLDLINEIAAHPLIINETPNLSYSS
                     SLYLEKTKSWIFGLTCQYEKQYIAQKKLYHLLATNEQENLDTKYVQIYLTEVLDLALF
                     SAKFGNNTECNFLTDYVLSKAPAVGESSSLFNLYPQFIKALSPFYKKEIPKLQTLVNE
                     YEPVLYEQFEKLPIQARDNVRLGLMRIFMLLNNHTKIKQWHYLLNEPKVYTRLDANLV
                     CHIVYTCSLYEQSTISVNKQNINTIPGLRKQVLELKKLCQNRPNTSPVESAIINTFIK
                     IMDTNLIQFHIMELNNLMKFLGTEKNQNALYLKRFLNMFDLSEWIQNQLMKMNT"
     gene            5954..6265
                     /locus_tag="PLB46_06645"
     CDS             5954..6265
                     /locus_tag="PLB46_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76230.1"
                     /translation="MARVVLKLRKRSTAEIIEFARGIVADAAINADFVYPQTSLYTIT
                     AVATLLEQSMLEAINQDQEKRAMVRQRRAELERVIIELANYINNEIKKNNLIEVPSIF
                     K"
     gene            6369..6941
                     /locus_tag="PLB46_06650"
     CDS             6369..6941
                     /locus_tag="PLB46_06650"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HRF76231.1"
                     /translation="MKKINLPFVFALILLLSLNSCTRFYGIKKPIEFPMESNDELNRA
                     SLSEEKMQLPILINDASIKSREQDIDVVLLANNEKTSTIITPMQKNILSAHDVKQNVV
                     PVTKVTTAPSTPEEKMDGFAVAGFVTGLVGMLFAGIVLGIVAIILSSIALTRIKKSDV
                     PKRGKGLAVAGLILGILGLVLSVILLAVLL"
BASE COUNT         2379 a         1227 c         1239 g         2626 t
ORIGIN      
        1 cattttcaaa ttggcgctat ggatataaaa tattcttaaa tcttacacct ggaactctgg
       61 atttaactcc tcgaggacgg gcgaaaaata tttcgcccct tcccaatcgg aacattccac
      121 tttcattttc aaattggcgc tatggatata aaatattctt aaatcttaca cctggaaccc
      181 tggacttaac tcctcgcgca cgggtgaaaa atatttcgcc caatgaattc aattaaaaaa
      241 attctctccc cacggggcat tcatatcaaa accatttaaa tcattttgaa ctggctaaat
      301 atatttcgtc attacggcat acttcaaaat cctgctgtat tgataattaa taaatatata
      361 aaacattgat attcaataat atattaataa tcgctttgaa attttttgga ataaaacaat
      421 atataatgcg ttgcattagt atttagacgc gtgcagcgat tttgttattg ataaatcaaa
      481 tggcgatgca tatccacttg gttttgccat ctaccaaaat tttatttcca gatatcaagt
      541 tgcaaccgga caaatggtgt tcacacaaaa tgtttcccca ataacttgga tgttgattta
      601 gaaaaactac cggttcgatt ttacattttg ccgattaata ttgagggaat atttgaaacc
      661 aggggttttg tgaagatgta aaacgtaata aggtgtttag aaggctgtcc aattcatatt
      721 taaagaaaaa tttaacaaaa aaattgttta aaaaaaaacc ggcaattgcc ggtttttaaa
      781 ttacttaggt ggaaaaagaa tgtcccacca tgggaataaa aatgaaagca ccggaaccag
      841 tgcggccaac ttaccggcct tgccggatag gttgttcaac attgtttgta tcataaaatc
      901 aaattttatg atactaatgt cactacgatt ttagcggata ctatgggtat tcgaccaaga
      961 ggtaaaagat tcgatgaaat cgaggtttaa tcgacaaaag tgtttattta agaaaaatta
     1021 ttttctttat tttctctcta gcctgagtaa aactaaccgg aacctgcaat agattactca
     1081 ttatgattat gttgaactgt ttttctgctt tttctatgtg atcgctattt attaaataac
     1141 ttttactggt tcttattata tttgaatagc cacttttatt aaaatcatct tctaatatgt
     1201 tcaaacgttt tgagacagtt ttattattta gttttgtatt cgatgaatac aggatggaat
     1261 aatctttatt cccttttagc gcgatgattt ctgaaatttg tatccgaaaa atcgggttgt
     1321 ttaactcggg taaataaatg tatttgcttt caatggttac aacattattt cgacgctttt
     1381 cccaggcgcg tatggcttcg ataaaatcat cttcggtaaa cggttttgtc aaataatact
     1441 tggcgcctag attcaacgct tgaacacctt tatccagacg tgaggtataa attacattta
     1501 agttgaggtt gtggtaaaat ttaaggacgt caatacctgt ttttgagccg cttagttcta
     1561 tatctaaaaa cactaaatcc ggtttatggt tttcaatagc ttccaatgcc gcagcataac
     1621 tatctgcaac cgccctaatg tttagcaacg gataaaattt gttgagtaaa tgcaccaaat
     1681 cagtgacatg ttgtgggtta tcctcgataa ttacaattga accttgcatt acatgagttt
     1741 tacagatgat ggaataatta attcaatgtt gctaccttga tttatgtgat ttattttaat
     1801 aaaatccttt attttcttct ttttacattc aatacgcaag cgttcataaa taatttgtat
     1861 cgcacttggc tctttgtttt gtaaaattga ttcaagtgtt atgtctttat cgaatccggg
     1921 cccatcgtca ataacattaa ttttaataaa cgattcatac acatttatgg caacctctat
     1981 attaataatg taattactag cttccacagc atgtttaatc gtgttttcta ccaaagtttg
     2041 aattgcgaga gggggtttca tgacaatgtc atgtaaaggg gcgttttcag gaactatttt
     2101 aatcgagtaa ttaaacctat tttcaaggcg gaggccttct attttgataa acttctcaac
     2161 tatgtttaac tcttcacgaa gcgtaatctt atctattttc gcaaaatgga agatatctga
     2221 agtaatagag gaaagtagat cacctcgcag aattgctcgc tcgggattct ctcgtgtatc
     2281 gcctttatta agtgctatgt tattagcaat aaaatgcgtg ttcatccaat acgataaaaa
     2341 attgttttta atttgcaaca ccttcttttt accaatttgc ttcactagaa taataattga
     2401 aaaagcaatc acaaaaaaca agatactgaa caatacaaac caattttttt gcttagctct
     2461 tttttgttgt tccaactccc tttgttgttt ttctgattga gcttttaaca aggccagact
     2521 tttttggtcc tctgcttgtt ttacttttgc tttttccaat tcaaaggctt gattagaaag
     2581 ttctgcagtt cgctggagcg aatcattgat aagtgtgatt acccggttct gtgtttgttg
     2641 ttctaataaa gttatagatt gtaatcttag gttacgctca accaattcat ttctcaaatt
     2701 agtttcaacc aacatcttgt ccttttctgc cagctggtat tttttgttta aagtctcaat
     2761 ggtgaaattg ttttcaatat ttcttaaact gtctttgtat attttaccag tgatataatt
     2821 ttccaacgcc gctttataat tccccttttc taaatttatt ttataaagca ggttgttagc
     2881 catcgaaatt tctttttgtt gttctgctgt ttcggcaata gctaacgcaa tatttgcaaa
     2941 gctatccgct aaaattaaat tgttggtaag ttgatactcc gcagcaagac tatattttgg
     3001 taaaaatttt gcggtaaata ccgaaactcg ctcactcacc acatcagctt ctataaaata
     3061 ttgtaaagca gagtcgtggt tgcctagccc tgataacgca gtagcttttg atattaataa
     3121 tgaccacgtt gtagcaatgt tgtttgtttg ctcgcaaatt gcaatcccgg cattagcatt
     3181 ttccagagct ttttcgtagt caccactcct attttcgatg ttggcaatag aattatacac
     3241 cctcggaata agattattat attggtattt tatacatata tctaacatgc tatataagta
     3301 tattttacca tctacgtaat ttcctttatc catcaacaaa tttccaatac gttgctttac
     3361 aatggccagg ttataaaagt catattccct ctcaaaaaga gggatgcact tttcgtagta
     3421 ctctatagcg ggtttgaata aactttttgc tttgtaaata tcacctatat ctagatatgc
     3481 ttgagcgatt gttatattaa acaggtttgg cgttacgtta atcattgaat cgcactccat
     3541 taaaatacca tagctgctat ctaaattccc ttttaatttg taagcctctc caattttagc
     3601 ataggtacta attgttcgat taggatcatt tgctaattta aaatattcaa gcgcagttga
     3661 atataactga atcattgaat cataattgtt ttgatcggaa tataattggg ccagatcata
     3721 atatagtctg ccaataaaat aatattcatc atttccaaat ggttttgcag catttaaata
     3781 atacaaggca gaatcaaatt cgcctaaata gttttgaatt tccgctttaa ctataaaacc
     3841 ctttaaagga tagtaataat cagaatagaa attattattt gaaatggctt tatttattaa
     3901 ttcatctgcg gcatcaatgc tatcaataaa taacaaaagt tgtgctttgt taatttgagc
     3961 ataaatttta ggattttgat aaccgtataa atcagccagt tgaataatac tgtcgtataa
     4021 taggagcgct ccgtattcat cggtatttga caaaatgtcg gctgtgtcta tcagattaaa
     4081 gaggtggttg gagtcgatga cggtaagggt attctttgca ttagactcgc ttatactgtt
     4141 ttctatggaa aaaaagaatg cagaagcggc aaacataact agtaatatcg ttttcatagt
     4201 tgctgggttt ttaagtgttc atcttcatca attgattttg tatccattca gataaatcga
     4261 acatattaag aaatcttttt aaataaagtg cattttggtt tttttctgtt ccaaggaact
     4321 tcattaaatt gtttagttcc ataatgtgaa attgaattag gttggtatcc atgattttta
     4381 tgaatgtatt tataatggcc gattcaacag gcgaggtatt aggcctgttc tggcacaatt
     4441 tttttagctc aagaacttgt ttccgtaaac cgggaatggt atttatattt tgtttgttga
     4501 ccgatattgt tgattgttca tataatgagc acgtgtaaac tatgtgacaa actaaatttg
     4561 cgtctagtcg ggtatatacc tttggctcgt tcaataaata gtgccactgt tttattttgg
     4621 tgtgattgtt taataacatg aaaattctca tcaaacccag ccttacgtta tctctggcct
     4681 gaattggcaa tttttcaaat tgttcataca aaacgggttc atactcgttt accagggttt
     4741 gtagtttcgg tatttctttt ttgtaaaatg gagatagcgc tttaataaat tgtgggtata
     4801 gattaaatag ggaagatgat tcgcccaccg ccggagcttt gctcaaaaca tagtcagtta
     4861 aaaagttaca ttcggtgttg ttcccaaatt ttgcagaaaa aagtgcaagg tcaagcactt
     4921 ctgttaaata tatttggaca tacttagtat ctaagttttc ctgttcattt gttgctaata
     4981 aatgatataa ctttttttgt gctatatatt gtttttcgta ttgacaggtt agcccaaaaa
     5041 tccaagattt tgttttttct aaatataaac ttgaagagta ggataaatta ggtgtttcat
     5101 taataattaa aggatgggct gcgatttcgt ttattaaatc caggtcactg ccatcgttta
     5161 atatagcagt tttaaataat gtaagcgctg tatgagtatt taatttatta attaaaaaaa
     5221 attggtttaa atcttgggca tcctgctcaa tttggtgcaa taaataagat acgttttctt
     5281 ctaagttttt gcccgaaact tttggcgtta tgtttaacag tttttgcaaa atcatacttt
     5341 tgtagaaagg cgaaacacaa tgattagttt caattaacgc ttgttttaga gccttatgcg
     5401 attcttcata catccccttt gatgatagta tttctgcgtg gtagagcagc ctttctgctt
     5461 cgcctcctaa gtacttaaaa ttgctcttta ccaacacatc caggataata cctttcagaa
     5521 tataccttgc atcagtaaat gctttacttt ccatgggctt agatttttct ttaaactgcc
     5581 ttctccaagc ataccctata gtttcagtgt tatacgcact taccgactgt aacgcacgaa
     5641 acaattttgc cggatcgctt tgtaatgcgc gagggcccct ttgtttaaat gaaaattcaa
     5701 cttcacgtat ttctaggggg gtcatatttt gaatgagcgt ccaaatgtta aaaatattat
     5761 ccattgctaa attgagtgtt tattcagaac tatgcaaaac gcatattata tttcaatata
     5821 tccgctaaaa cattgataat caatatatta aaaaacttat taccgattaa taatcgaaaa
     5881 tagcaataaa ttatacattg tttggtttta agcacagaaa tatttttagc cttgaaaatg
     5941 gcagcaagaa gaaatggcta gagtggtatt aaaactcaga aaaaggtcaa ccgcagaaat
     6001 tattgaattt gccagaggca ttgtagcaga tgctgcaata aatgcagatt ttgtttaccc
     6061 tcaaacaagt ttatatacca taacggcagt tgcaacgcta ttagagcaat cgatgctgga
     6121 agctatcaac caagatcagg agaaacgtgc catggttaga caacgaagag cggaattgga
     6181 aagagtaata attgaacttg ctaattacat caataacgag attaaaaaaa acaatttaat
     6241 cgaggtacca tcaatattta aatgaggaat gataatacat agacactggt gcttaatgtt
     6301 tgtaatatta agacagggcg gtcatattaa aaaatataat ttttaaacca ttaataaaaa
     6361 aaagaactat gaaaaaaatt aatttaccat ttgtgtttgc acttatttta cttttaagcc
     6421 taaactcctg cacacggttt tatggtatta aaaaaccaat tgaatttcca atggaatcta
     6481 atgatgaact taatcgggca agtttatctg aagaaaaaat gcagctacct attttaatta
     6541 atgatgcaag catcaagagt cgggaacagg atatcgacgt agttttatta gctaataacg
     6601 aaaaaacgtc aactattatc acaccaatgc aaaaaaatat tttaagtgca cacgatgtta
     6661 aacaaaatgt tgttcctgta actaaagtta ccacagcacc gtcaacacca gaagaaaaga
     6721 tggatggatt tgcggttgcg ggttttgtaa caggtcttgt aggaatgtta tttgcaggta
     6781 tagttttagg aatagttgca atcattctta gttcaattgc cttaactcga attaaaaaaa
     6841 gcgatgttcc gaaaagagga aagggtcttg ctgttgcagg tctaatttta ggtatactgg
     6901 gcttagtctt aagtgtaata ttgttagcag ttttattgta atatgacaat ttttgttaac
     6961 tcaaagtatg taattaaagg taaaatacaa ttccgataat acttgtcttt ttcaatatag
     7021 aaaacggaac taaaaataaa agaactattt gataacgaat aaatggcatt gttcctcctt
     7081 gtaatattta gttttagcat agacttgaaa cgatgattac tctaaaatat taatgttgca
     7141 gcccatggcc cgtaattttg ggtttacttt ataacaaatt agcaataaaa ttaaaaatct
     7201 ctaaccagct ctgcagaaaa caattctgcg gagcttaccc agagatttat tgaaacgtgc
     7261 catattggat ttttatacgt tgaatttagt attgaattaa ttgcattggt tttaattaat
     7321 tgtttttcct ccaccggatt ttaatttatg tatttttacc aattaagaaa aaacaatgaa
     7381 aacccggtcg gatcaaaata ctattaatgc atcaaacttt ttcctctccc tcccacaacc
     7441 cacacaatct acttttctag ccctgagcaa t
//
LOCUS       DAORKW010000126         7461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_309_length_7461_cov_4.471240, whole genome shotgun sequence.
ACCESSION   DAORKW010000126 DAORKW010000000
VERSION     DAORKW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_309_length_7461_cov_4.471240"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..621)
                     /locus_tag="PLB46_06655"
     CDS             complement(73..621)
                     /locus_tag="PLB46_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76232.1"
                     /translation="MKKLFTLSFGILLLTQVKLSAQTNIFPATGSAGIGTTTPVSSAI
                     VEMQSTSQGLLAPRMTKAQRDAIAAPVAGLLIFQTNSQPGFYYYTGAGWTAMAAKGAN
                     TSLSNLAASTAINTGLTPNANNTLDLGSSTANWNELYVNTIKFMDGSTQTTAGGGGGR
                     SSAASLRGAMGRWTAGATRSTR"
     gene            complement(869..1522)
                     /locus_tag="PLB46_06660"
     CDS             complement(869..1522)
                     /locus_tag="PLB46_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF76233.1"
                     /translation="MENIRVAIFEDNKHLRESLYYLINGTPGFSCTGAYPDCNDAVFQ
                     ITKDTPDVILMDIEMPGLNGIQGVKLIKSKFPQVHVLMQTIFHDENNIFDAICAGASG
                     YILKSTTPAEYITAIKDVYNGGSPMTGSIARKVLELFQKNIQVEAKQDYQLTPKEKEM
                     LQFMVQGKSFKMIAEAAGISYETVRTHMKNIYAKLHVNSNTEAVSKVLKEKLLSIFF"
     gene            complement(1578..4514)
                     /locus_tag="PLB46_06665"
     CDS             complement(1578..4514)
                     /locus_tag="PLB46_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF76234.1"
                     /translation="MFAQEYLVKSFGAKQGLASAEIYTLFQDDDGYIWLGTRLGVSKF
                     DGISFVNYNGSNDLRFGKVSAITQDANRTIWIGCENGLFYFEEGIFKHVAFDSRFPDS
                     WIYALNADVENKLWIGTSDGPVLIEEQQIQKIKSGDTLAFKLLSNWSQLSDPSNQVFT
                     ITTKKQNNEIITYFGTRNSVIQLKNNEFAIRWFSREPRLEDVSAIKVTNHNKLLIGNK
                     SGFYYKESNTGFDTIHDFTYATGMTTINDSVFYVLGFEGIYSITGNTTRKLTEIASLG
                     YDSPSCILYDKEQNLWIGTWEGLVQLRKNTITTWLPGKTPNLNDMFSVTEAPSGEMVF
                     GGNRGNVLVFENNQFNKYFGLTQKPWPLSEVFGLYFSDNLVWMGSGYQGLSVWDGKNM
                     FNYAGEKLKDEHAQGFFRDEKNNLFVLSDGGLTQVLNPDNPSELAFKYFPWGIDIGGQ
                     YLKIFDYAILNNNAIYLATNFGVVYFNGDTLLQVTTDNALLNDALITSMAIYDDQTIW
                     LATGNYGLFKIQPDKTQAKVVFQLSETDGLLANAVLDLVADEKRNYLWCAHYNGLTVV
                     DLNNKNPKVVKRITKDEGFLPYDFTYCKLALQQKADCIWMATTAGVQRLNINEMPRNN
                     VPATPIIRNVLLFNNADAVGAYADEKELISGIWLNPKFPHNKNAISFNFQSLSLVIPE
                     LNRCRYKLIGYNEEWVYTKNEGEVTFAALSPGSYTFVLEAANNDGLWNTTPASYSFTI
                     TKPYWATWWFILVALITVILISYGIYRYRINQLIKIGNIRNKIAGDLHDDIGSTLSSI
                     SMYSEIVRNQIGNKTDVNDELLKKITQNSKEMVDNMSDIVWAIKPENDRFKNIESRMF
                     NFATEMCNLKGVELKMDKSNIDGDIKIRMEERRDFYLLFKEAVNNAIKYAQCSTLMVN
                     FNIEQKTLVMTIADNGKGFDVNQAQQKGNGLNNMVVRARQHNGDCLISSAIGKGTVVT
                     VLWPLT"
     gene            complement(4629..5642)
                     /locus_tag="PLB46_06670"
     CDS             complement(4629..5642)
                     /locus_tag="PLB46_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF76235.1"
                     /translation="MEKYPQAEQLLLPIYDSAVVHKFYDEQFDIAGTLSSLYAETGNY
                     EKAFLFLKQLNAAKDTIDNRKQNEQLQALQEQYTADQREKEIALLNATNNLQQKEIER
                     KNLITILLVVIFGALVVSGFVIWNRYTLKQQLKEVQLRNKIAADLHDDVGATLSSIKM
                     YSEIIKSNGTDLQTEQDHLLNKIINNSSESIESMSDIVWMVKPGNDKFSSLNNRIKHF
                     AEEVCSTNNIEHTTNFQPELAQLTLPMDMRRDVYLLIKEAVNNAAKYAHASQLDIVIT
                     YNNDTLQIAVTDNGKGFDTSADVHGNGLSNMHARATKYGGSCTIDSRQGLGTSVYVQL
                     PVK"
     gene            complement(5707..6534)
                     /locus_tag="PLB46_06675"
     CDS             complement(5707..6534)
                     /locus_tag="PLB46_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF76236.1"
                     /translation="MKRNYSIGIIVLSLILMSGFTVSAQQLNIDSLIHITKTGVDDTN
                     KVIAFRALCGATSNSNPQQSIQYGWQGVALSKKLFWDKGTAGCMLNVSIAYSNLGKYD
                     SAVLILDTALVYAKKVGEEKRIALIYINQASAYIYMGKLDDAMQVALNAVPYAEKSGD
                     ADRLARVNMTIGNIYFYQEKWKNAESYYAKSIPLFEQLSNENMVGVVTMNMSISQKNR
                     NSLDTAEIYAYKSIDILQRKNDIENLILAHTNLGDLLSYKKKLYRCRKGIPVIHQSG"
     gene            complement(6531..7223)
                     /gene="ung"
                     /locus_tag="PLB46_06680"
     CDS             complement(6531..7223)
                     /gene="ung"
                     /locus_tag="PLB46_06680"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRF76237.1"
                     /translation="MLSQTGNVIKIEDSWKEVLMDEFDKPYFKEIRQYLKSEKASRKV
                     IYPPGPLIFNAFNTTPFHKVKVVIIGQDPYHGEGQAHGLCFSVQKGIKPPPSLVNIYK
                     EMKSDLGLNPPPHGNLQSWAEQGVFLLNAMLTVEKDQPASHQKIGWQNFTDVAIQKLS
                     DHRDKLVFILWGAFAQQKALLIDSNKHLIIKSPHPSPFSADKGFFGSKPFSKTNQWLE
                     RNGITAVNWQLT"
BASE COUNT         2234 a         1451 c         1252 g         2524 t
ORIGIN      
        1 gcccgcgtgt gggctgcagg aacttggtca gcaggttgaa gcaggtcgtc ttgccggcgc
       61 cgttcgggcc gatcagcgcg tggatcgtgt ggcgccggct gtccagcgac ccattgcgcc
      121 ccgcaacgaa gccgcggaac tccttccacc acccccgcct gcggtagtct gtgtggagcc
      181 atccataaat ttgatggtgt ttacataaag ttcattccaa ttagcagttg aagaacctaa
      241 gtcaagtgtg ttgttggcat ttggagttaa gccggtattt attgcagttg atgcagctaa
      301 attagacaag gatgtattcg ctccctttgc tgccattgct gtccaacccg ctccggtata
      361 ataataaaac cctggttgcg aattggtttg aaaaataagc aaacctgcta ccggtgcagc
      421 tattgcatcg cgctgcgctt ttgtcatacg cggagcaagc aacccttgtg aggtagattg
      481 catttctaca atagcactgg aaacaggtgt tgtagttcca attcctgcgc ttcctgttgc
      541 gggaaaaata ttggtttgtg cggataattt tacttgagtc aagagtaaaa ttccaaatga
      601 taaggtaaat agctttttca taaattgaga tttaattatt catctcaaaa gtgcaactgt
      661 tttaaaaggt aagaaatacc acaatagggg gagcgaccaa aatgaaggct gactaaaaaa
      721 acagtagtaa gggaatatgg cttattgtaa atcccttgtt tttatttatt aaataagact
      781 gtgttgtaat gtgggagtta caagtttttt caataataaa tagtgagtta catttagtaa
      841 ttaaacgaag aataagcggg aaaatgtatt agaaaaatat cgataataat ttctccttca
      901 gcactttact aactgcttca gtatttgaat taacatgcaa tttggcataa atatttttca
      961 tgtgcgtgcg caccgtttcg taactgatac ctgccgcttc tgcaatcatt ttaaaacttt
     1021 tcccctgcac cataaattgt aacatttctt tttctttagg agtaagctga taatcttgtt
     1081 ttgcttcaac ctgaatgttt ttttggaaga gttccaaaac cttacgggca atagagcctg
     1141 tcattggtga gcctccattg tacacatctt taattgccgt gatatattca gcaggagtag
     1201 tactttttaa aatataaccg gaagcacctg cacatatagc atcgaaaata ttattttcat
     1261 catggaaaat agtttgcatg agcacatgta cctgagggaa tttgctttta atcagtttaa
     1321 caccctgtat accatttaat ccgggcattt caatatccat tagaataaca tcaggagtgt
     1381 ctttagtaat ttggaaaacg gcatcattac aatcaggata ggctccggtg caactaaaac
     1441 cgggtgtgcc gttgattaaa taatataaac tctcgcgaag atgtttatta tcttcaaaaa
     1501 ttgccactct gatgttttcc attgtcgttg attataaaac aaaaatacaa tatacgattg
     1561 tttaatatat accacaatca ggttagcggc cacaaaacag ttacaacagt acctttacca
     1621 attgctgaac taatcaaaca atcgccgtta tgctgacgtg ctcttaccac catattattt
     1681 aatccattcc ctttttgttg cgcctgattt acatcaaaac cctttccgtt gtcggcaatg
     1741 gtcataacaa gtgttttttg ttcaatattg aaattaacca ttaacgttga acattgtgca
     1801 tatttaatgg cattatttac tgcctccttg aaaaggagat aaaaatcacg gcgttcttcc
     1861 attctaattt tgatatctcc atcaatattc gatttatcca ttttcaactc cacacctttc
     1921 aggttgcaca tttcagttgc gaaattaaac atgcggcttt caatattttt aaatctatcg
     1981 ttttcaggtt ttattgccca tacaatatca ctcatattgt ctaccatttc tttggaattt
     2041 tgtgtaatct ttttaagcag ttcatcattt acatctgttt tattacctat ttgattacgg
     2101 acaatttcgc tatacatact gatactactt aaggttgaac cgatatcatc atgtaaatca
     2161 cctgcaattt tatttctgat attacctatt ttaattaatt gattaatccg ataacgataa
     2221 ataccataac tgatcagtat tactgtaatt aatgccacaa gtataaacca ccatgttgcc
     2281 caataaggct tggtaatggt aaatgaataa ctagcaggag ttgtgttcca aagtccatcg
     2341 ttatttgcag cctcaagcac aaatgtgtaa ctgcctggcg ataatgcagc aaatgttact
     2401 tcaccttcat ttttggtata tacccattct tcattataac caattaattt gtatcggcag
     2461 cggtttaatt caggaataac caaagagagc gattgaaaat taaaagatat cgcattttta
     2521 ttatggggga atttaggatt gagccaaatt ccgcttatca actctttttc atcagcatag
     2581 gcaccaactg catcagcgtt gttaaataaa agtacattcc gaattatagg tgttgcggga
     2641 acgttattgc gcggcatttc attaatattc agtcgctgaa caccggccgt ggtagccatc
     2701 catatgcagt ctgctttttg ttgtaaggct aatttgcaat atgtaaaatc gtatggtaaa
     2761 aaaccttcgt ctttagtaat tctttttact actttaggat ttttattatt taaatcaaca
     2821 accgttaatc cattataatg agcacaccac aaataatttc ttttttcatc ggccactaaa
     2881 tccagaactg cgtttgccag taaaccatct gtttcagata attgaaatac cacttttgcc
     2941 tgcgttttat caggttggat tttgaaaagg ccataatttc ccgttgccag ccaaattgtt
     3001 tgatcatcat atattgccat cgaggtaatt aatgcatcat ttaatagtgc attatcagtt
     3061 gtaacctgaa gtaatgtatc gccgttaaaa tataccacac caaagttggt agctaaataa
     3121 atagcattat tattcaatat agcataatca aatattttca gatattgtcc accgatatca
     3181 attccccaag ggaaatattt aaacgctaac tcagatgggt tatccggatt taacacttgt
     3241 gtgagtccac catcgcttaa aacaaaaaga ttgttttttt catcccgaaa aaaaccttgt
     3301 gcgtgttcat cttttagttt ttctccggca taattaaaca tgtttttccc atcccaaacg
     3361 ctaagtccct ggtaaccact tcccatccaa actaaattgt cgctgaagta cagcccgaac
     3421 acttcagata atggccaggg tttttgcgtt aaaccaaaat atttattgaa ttgattgttt
     3481 tcaaacacga gaacattccc tctgttgcca ccaaatacca tttcaccgga aggtgcttca
     3541 gtaacagaaa acatatcatt taaattcggt gttttacccg gcaaccaagt agttattgta
     3601 tttttcctaa gttgcactaa accttcccag gttcctatcc agagattttg ttctttatcg
     3661 tataaaatac aggaaggact atcataacct aagctggcaa tttcggtcag ctttcgtgtt
     3721 gtatttcctg ttatactata aataccttca aatcccaaca cataaaaaac tgaatcatta
     3781 attgttgtca tgcccgttgc ataggtaaaa tcgtgtatgg tatcaaaacc ggtattactt
     3841 tctttataat aaaatccgga cttattgcct ataagcagtt tattatggtt ggtgacttta
     3901 atagcgctga catcttctaa acgtggttca cgactaaacc acctaattgc aaactcatta
     3961 ttttttaatt gaataacaga attccgagtg ccgaaataag ttatgatttc attattttgt
     4021 ttttttgttg tgatagtaaa tacctggtta ctaggatcgg atagttgcga ccagtttgat
     4081 aataatttaa atgccagagt atcgcctgat ttaatttttt gaatttgttg ttcttctatc
     4141 aaaacaggac cgtcagatgt accaatccaa agtttatttt ccacatcagc gtttaacgcg
     4201 taaatccagc tatcaggaaa gcgtgaatca aatgctacat gtttaaatat tccctcttcg
     4261 aagtaaaaca aaccattttc acaacctatc caaattgtgc gatttgcatc ttgcgttatt
     4321 gctgatactt ttccaaatcg caaatcgttg ctgccgttat aatttacaaa ggaaatgcca
     4381 tcaaatttac ttacccccaa acgtgttcct aaccatatat atccatcatc atcttgaaac
     4441 aacgtataaa tttcagcact ggcaagtcct tgttttgcgc caaatgattt aacaaggtat
     4501 tcttgggcaa acaaagaaat actgttaaaa caaaagacga aaaataaaaa aaacattatt
     4561 cgggtataca acctgttcaa catatacgag tgcctgaatg aataatcata aagttacttc
     4621 attttgattt atttcaccgg taattgaaca taaactgatg tgccaagccc ttgacggcta
     4681 tcaatagtgc agcttcctcc atattttgta gctcgggcgt gcatattgga caagccatta
     4741 ccatgaacat cggcggaagt gtcaaaacct tttccgttgt cagtaactgc aatttgcagt
     4801 gtatcattat tgtatgtaat gacaatatca agttgactgg catgagcata ttttgctgca
     4861 ttgttaacag cctcttttat caaaagatac acatcgcgac gcatatccat tggcagtgtt
     4921 aactgcgcta attccggctg aaagttcgtg gtatgttcaa tattatttgt actacatacc
     4981 tcctcagcaa aatgtttgat gcgattgttt aaactcgaaa atttatcgtt cccgggtttt
     5041 accatccata caatatcgct catactttca atcgattcgc tggaattatt tataatctta
     5101 tttaacaaat gatcttgttc agtttgtaaa tccgttccgt tagatttaat tatttcactg
     5161 tacattttaa tactgctgag cgttgcacca acgtcatcgt gtaaatcggc ggcaatttta
     5221 ttacgcagtt gcacttcctt taactgctgt tttaaggtat atctattcca aatgacaaac
     5281 cctgaaacca ccaaggctcc gaatataacg acaagtaata tggtgatgag gtttttacgc
     5341 tctatttctt tttgttgcag attgtttgtc gcgtttagca atgcaatttc cttttcacgt
     5401 tggtctgcag tatattgttc ctgtaatgcc tgcagttgtt cattttgttt tcgattgtca
     5461 attgtatctt ttgcggcatt taattgtttt aaaaataaaa atgctttttc ataattgcct
     5521 gtttcagcat acaatgatga aagtgttccg gcaatatcaa attgttcatc gtaaaattta
     5581 tgcactactg ccgaatcata tattggtaac aataactgtt ctgcttgcgg atatttttcc
     5641 atttgaaaat ataaatcgcc aagcgattgt ttattggcaa tctgttgttc aaaatcacca
     5701 atttgttcag ccagattgat ggataacagg tattcctttt ctgcaccggt ataatttttt
     5761 tttataactc aataaatcac ctaagttggt atgggccaat attaaatttt ctatatcatt
     5821 tttccgttgg agtatatcaa tagatttata ggcataaatt tcagcagtat ctaaactatt
     5881 tcgatttttt tgagaaatgg acatattcat cgttaccacg cccaccatgt tttcattaga
     5941 caattgttca aacagaggta tactttttgc gtagtaactt tcggcgtttt tccatttttc
     6001 ctgataaaaa taaatattgc caattgtcat gttaacccgt gctagtcggt ctgcatctcc
     6061 cgatttttcg gcatacggca ctgcatttaa cgcaacctgc atggcgtcat ctaatttccc
     6121 catataaata taggcagatg cctgattaat ataaatgagt gctatacgtt tttcttctcc
     6181 aactttttta gcatacacta aagccgtatc caaaatcaaa acagcagaat catattttcc
     6241 taaattacta taggcaatac ttacattcag catgcagcct gcggttcctt tatcccaaaa
     6301 taatttttta cttaaagcaa caccctgcca gccgtactga atactttgtt gaggatttga
     6361 attagatgtt gcaccgcata atgcacgaaa tgctattact ttattggtat catccacacc
     6421 tgttttggtg atatgtatta atgaatcaat attgagttgt tgtgctgaaa ccgtgaaccc
     6481 gctcattaaa attaaggata atactataat gcctattgaa taatttcttt tcatgtaagc
     6541 tgccagttta ctgctgtaat gccgttacgt tccaaccatt gatttgtttt ggaaaaaggt
     6601 tttgacccaa aaaaaccttt atcagcacta aatggcgatg gatgtggtga ttttattatc
     6661 aaatgtttat tgctatcaat caacaatgct ttttgttgtg caaatgctcc ccataatata
     6721 aaaaccagtt tatcacgatg gtcgctgagt ttttgaatag caacatcggt aaagttttgc
     6781 catcctattt tttgatgact ggccggctgg tctttttcta cagtaagcat ggcatttaat
     6841 aaaaataccc cctgttctgc ccaagattga agattaccgt gcggaggcgg gttcaaacct
     6901 aagtctgatt tcatttcttt ataaatattg actaaggaag ggggtggttt tatgcctttt
     6961 tgcacactaa aacacaatcc atgtgcctgt ccctcaccat gataagggtc ttgaccaata
     7021 atcaccactt taaccttatg aaacggagtt gtattaaaag cgttaaaaat gagcggtccg
     7081 ggaggatata ttactttcct gctggccttt tcacttttca gatattgcct gatctcttta
     7141 aaatagggct tatcaaattc atccataagc acctctttcc atgaatcttc tatctttatt
     7201 acattgcccg tttgagataa cattttgtaa acttagtagt attggctcaa ttatttaaaa
     7261 aaactgattt aaatcacttg ttaaaaaatt gttttttact acatttgaaa aacaagccag
     7321 cttatgggag aaacatcctt aaagataaaa aaaaccgcct caaatcacat caagtttgtc
     7381 aatgcattgc ttaatgatat tgaagcgctt gaaatgatga ttaccgctaa cctttttgag
     7441 tccgacaaca ttagaatagg t
//
LOCUS       DAORKW010000127         7396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_313_length_7396_cov_4.820188, whole genome shotgun sequence.
ACCESSION   DAORKW010000127 DAORKW010000000
VERSION     DAORKW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_313_length_7396_cov_4.820188"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..3156
                     /locus_tag="PLB46_06685"
     CDS             34..3156
                     /locus_tag="PLB46_06685"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRF76238.1"
                     /translation="MKKQFSFLSVLLICLLFVQTIAAQTGSSSSQGSTNNPGTNTNKP
                     PTTGSSSSSSSTAGSTNGGPIRVEPGTPDPITLFTSGNATAAKPGTPNPGKYSFITIP
                     DDPTRTRIYKLNNGFTVYLSQNKAEPRIQTYIAVKAGSKNDPRETTGLAHYLEHMMFK
                     GTSNVGTTNWSREKPIIDAIAQLYEQHRKEKDPEKKKEIYKKIDEMSQEAAKYAVPNE
                     YDKMITSLGAKGTNAYTSKEQTVYVNDIPANELEKWLVIESERFSTCVLRLFHTELEA
                     VYEEYNMGQDRDGSKMSEAYYRALFPTHPYGTQTTIGEGEHLKNPSMLNIMAYFYNFY
                     VPSNMAICLSGDIDYDKTIELINKYFGTYVNRPIGEQKFLPEKPLQEVQRREVFGQEA
                     ETMMLGYRVSGAGTEETMLAKLFTGILYNGQAGLIDLDLNQQQKVLDAGAFLSEWKEY
                     TVITFAADPREGQSLEECEQLLLAEIEKVKRGEFDEWLLKAVINDYKLSRIKSFESNS
                     SRASAFVNTFVKGIDWGSYLNEHKAMESVTKQDIIDFANEFFGSNNYVVVYKRQGEDK
                     NVKKVDKPAITPVEANREAQSAFLQKFMELPSLRVQPEFIDYEKRISNQTLGTNIPFS
                     YIKNEENQLFELYYILDMGTNNDLYLPLAINYLPYLGTDKYSPSDLQKEFFKYGLSFN
                     VFTSSDRVYVSLQGLESNLDKGVELFEHLLSSVKPDNKAYKELVNGIIKEREDNKSNK
                     SAIFNSALATYARYGSYSPVTDILSEKDLNNTLPTTLTDKIKSLTNYKHRIFYYGALP
                     QDQVITVLAKYHKAPETLAEYPVAKKYTEQSTKTNKVYFVNYNMVQAQVMFMAKDENF
                     NKSLLPEARLFNEYFGSGLSSIVFQEIRETKALAYSAYANFTTPANKDEAHYVRAFVG
                     TQADKMQSAIEAMMDIMNNMPQAEKQFNASIESVVKQIESERIIRSNIFWSYENAKRR
                     GLTTDIRKDIYTNVTGMTLPDLQQFVNDHIADNTYTIIVMGDKTKLDMSYLQSIGEFK
                     ELTLEEIFGY"
     gene            3379..4620
                     /locus_tag="PLB46_06690"
     CDS             3379..4620
                     /locus_tag="PLB46_06690"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HRF76239.1"
                     /translation="MAEVIRMPRMSDTMTEGTFVEWHKKVGDTVKSGDILAEVETDKA
                     TMELENYVKGTLLYIGVEKGKSAQVDQVIAIVGAPGEDFQALLNETPKAVTEQKTEEK
                     APATTETPVATNKTPVVETQEDGRIKASPLAKKIAQDKGINLDAVAGSGPEGRIIKKD
                     VEEFVPQTTEVAKAQQPIINIPQIVGEEGYDETPVSQMRKVIASRLSQSKFTAPHFYL
                     TISVDMDNAIAARKSLNEVSPLKISFNDLVVKASAMALRRNPAVNSSWLGDTIRTYKH
                     IHIGVAVAINDGLVVPVIRFADNKSLSQIAAETGDLAERAKNRKLQPADWEGNTFTIS
                     NLGMMGIEEFTAIINPPDACILAVGGIQQVPVVKNGQVVPGNVMKLTMSCDHRVVDGA
                     VGAKFLQTLKQYLEDPVRMLA"
     gene            4714..5076
                     /locus_tag="PLB46_06695"
     CDS             4714..5076
                     /locus_tag="PLB46_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRF76240.1"
                     /translation="MSKKTLVIGASNNPDRYAYKAVKKLTAYGHPVLALGKKEEMIDA
                     IPVVKERPLDTDIDTVTLYVNPQLQKNYYDYLLNLHPKRIIFNPGTENDELAELAQQK
                     GIQTLEACTLVLLSIGDY"
     gene            5489..6160
                     /locus_tag="PLB46_06700"
     CDS             5489..6160
                     /locus_tag="PLB46_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRF76241.1"
                     /translation="MSINISHTEENYLKAIFKLSEMQNGNVSTNAIAEKLSTTAASVT
                     DMLKKLAGKKLLHYEKYYGVSLTEKGKKLAKDLIRKHRLWETFLVEKLKFSWDEVDEL
                     AEQLEHINSEILTDRIDHFLGFPKFDPHGDPIPDKHGNIAYHEDVTLDKMQKGDEGII
                     AGVIDHSTEFLQYLNKIQLLIHTKIKVKDDEEYDQSKIIIINNKHEIVVSQKVAANLL
                     VRKIK"
     gene            complement(6184..6645)
                     /locus_tag="PLB46_06705"
     CDS             complement(6184..6645)
                     /locus_tag="PLB46_06705"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRF76242.1"
                     /translation="MGRTPPPYLVGNTQQPSVIYAPLKKRLVNYFIDHIFTLTVTILV
                     ASGCAINAIADIGCLFIVINFIYYTTMELFFSRTIAKFITGTHVTRENSLQRISLKQV
                     LFRSACRLIPGEPISFLVNKYGWHDKWSKTMVIDVQVKTNKQTPLPKNLVD"
     gene            complement(6679..>7396)
                     /locus_tag="PLB46_06710"
     CDS             complement(6679..>7396)
                     /locus_tag="PLB46_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRF76243.1"
                     /translation="LISGSRSSVPMLIGAAAFGMLCTVLIELFHKKAKLQEDASIGIS
                     FTWLFALGVILITYFADKVDLDQDCVLYGDITFINFETWNGIPTAVWLLGALFILIIG
                     FITIGYKGLYLTTFDPDYAITLGISVTLWHYLLMGAVSLTTVLSFESVGAILVVAFLI
                     VPGASAYLLTTKLKTMLLVAAIFGIVSSTGGYYMALWLNASVSGAMVSVLGVIFLVVF
                     CYTLIRKQLQTPVPQQDFAN"
BASE COUNT         2585 a         1358 c         1354 g         2099 t
ORIGIN      
        1 tattaattaa aataaaagta taaacactaa cttatgaaaa agcagttcag ttttttaagt
       61 gtattattga tttgtctact ttttgtgcaa actatcgcag cccaaacggg ttcatccagt
      121 tcacaaggtt caaccaataa tccgggcact aataccaata aaccacctac aacaggttcg
      181 tcatcttcaa gttcatccac agcaggttct accaatggcg gacctattcg tgttgaacct
      241 ggaacacctg acccaattac attgtttaca tcaggtaacg caactgctgc aaaaccgggt
      301 acaccaaatc ccggtaaata ttcatttatt actatccctg atgaccctac gagaacacgt
      361 atttataagt tgaacaatgg gtttaccgtt tatttatctc aaaataaagc agagcctaga
      421 atccaaacgt atattgctgt taaagcaggc tcaaaaaatg accccaggga aactacaggc
      481 ttagcacatt atctcgagca tatgatgttt aaaggaacat caaatgtggg tactaccaat
      541 tggtcgcgcg aaaaaccaat tattgatgcc attgcacaat tatacgaaca acatagaaaa
      601 gaaaaagacc ctgaaaagaa aaaagaaatt tataagaaaa tagatgaaat gagtcaggag
      661 gccgcaaaat acgcagtgcc aaatgaatat gataaaatga ttacctcatt aggtgcgaaa
      721 ggtaccaatg catatacgag caaagaacaa actgtttatg taaatgatat cccggctaat
      781 gaattagaaa agtggctggt tattgaaagc gaacgtttca gcacatgtgt tttgcgttta
      841 ttccataccg agttagaagc tgtatacgaa gaatataata tgggccagga ccgcgacgga
      901 agcaaaatgt cggaagctta ttaccgcgct ttatttccaa cacatcctta tggcacacaa
      961 acaacaattg gtgaaggaga acatttaaaa aatccttcta tgttaaatat catggcttat
     1021 ttctacaatt tttatgtgcc atcaaatatg gctatttgtt tgagtggaga tattgattat
     1081 gataaaacaa ttgaacttat taataaatat tttggcacct atgtaaaccg tccaatcggt
     1141 gaacaaaaat ttcttcctga aaaaccattg caggaagttc aacgtcgcga agtttttggc
     1201 caggaagctg aaactatgat gttgggatac cgcgttagtg gcgcaggaac ggaagaaact
     1261 atgttggcga aattatttac cggtatttta tataacggac aagcagggct cattgattta
     1321 gatttaaatc aacaacaaaa agtattagat gccggtgcat ttttgagcga atggaaagaa
     1381 tataccgtca tcacttttgc tgccgaccca cgtgaaggtc aatcactaga agagtgtgaa
     1441 caattgcttt tagcagaaat agaaaaagta aaacgaggtg aatttgatga atggttatta
     1501 aaagcagtaa ttaatgatta taaattatcg cgcattaaat cgtttgaatc aaacagcagc
     1561 cgtgccagtg cttttgtaaa tacttttgtt aaaggtatcg attggggttc ttatctgaat
     1621 gaacacaaag caatggagtc tgttaccaaa caagatatta tcgactttgc caacgaattt
     1681 ttcggctcca ataattatgt ggttgtttat aaacgtcagg gcgaagataa aaatgttaaa
     1741 aaggtggata aacctgccat cacacctgtg gaagcaaatc gcgaagcaca aagtgcattt
     1801 ttacaaaaat tcatggagtt accttcgtta agagtgcagc ctgaatttat cgattacgaa
     1861 aaaagaatta gcaatcaaac attaggcact aatatacctt tttcttatat taaaaatgaa
     1921 gaaaatcaat tgttcgaatt gtactatatt ttagatatgg gtacaaataa cgatttgtat
     1981 cttcctttgg caattaatta tttaccttat ttaggaactg acaaatattc accatctgat
     2041 ttacaaaaag aattttttaa atacggattg agctttaatg tatttacttc ttccgacaga
     2101 gtgtatgttt cgctccaggg cttggaaagt aatttagata aaggtgttga actgtttgaa
     2161 catttattgt cttccgttaa acctgataat aaagcctata aagaattggt aaatggtatt
     2221 attaaagaaa gggaggataa caagtcaaat aaatcggcca tatttaatag cgctttagca
     2281 acctatgcac gttatggcag ctattcaccg gttacagata ttttatctga aaaagattta
     2341 aataatacat taccaactac gttaacggat aaaataaaat cgttaactaa ctataaacac
     2401 cgcatttttt attatggtgc attaccacaa gaccaagtaa taacggtatt agcaaaatac
     2461 cataaagcgc cggaaacttt agcggaatat cctgttgcta aaaaatacac agagcaatct
     2521 accaaaacaa ataaagtgta ctttgttaat tataacatgg tgcaggcaca agtgatgttt
     2581 atggcaaaag atgaaaattt caataaatca ttattgcctg aagcgcgttt gtttaacgaa
     2641 tattttggta gtggattgtc cagtatcgta tttcaggaaa ttcgcgaaac caaagcatta
     2701 gcgtattctg cttatgctaa ctttaccaca cctgcaaaca aagacgaagc acattatgtg
     2761 cgtgcatttg tcggcacaca ggcagataaa atgcaaagtg ctattgaggc tatgatggat
     2821 attatgaata atatgccaca agcggaaaaa caatttaatg ccagtatcga atcagtggta
     2881 aaacaaattg aaagcgaacg tattattcgt tccaatattt tctggagtta cgaaaatgca
     2941 aaacgcagag gattaactac tgatatcaga aaagatattt acactaatgt aacaggtatg
     3001 accttaccgg atttacaaca atttgtaaac gaccatatcg cagataatac ttatacgatt
     3061 attgtaatgg gcgataaaac taaattggat atgtcctatt tacaatcgat aggtgaattt
     3121 aaagaattaa ccctcgaaga aatttttggg tattaatatt acaaccaaca aatgattgta
     3181 cttcgcaata aaataaactt gcgaagtaca atctctactg ttggcacttg atagcattta
     3241 atcctccttc tatccccaat tatagcttcg aaactttgcc tttgtgcccg gtttgcttac
     3301 cacgtgggca acattctaca aacccggttt ccaaccatcc aattttcttc ctattttcgc
     3361 actaaatctg attgaactat ggctgaagta atacgtatgc cccgaatgag cgataccatg
     3421 accgaaggaa ctttcgtgga atggcataag aaagtgggtg atactgttaa atccggggat
     3481 atactggcag aagtggaaac tgataaagca accatggagc ttgaaaatta tgtaaaagga
     3541 actttactgt atatcggtgt cgaaaaaggc aaaagcgcac aagttgatca ggttatcgct
     3601 atagttggcg ctccaggtga agattttcag gctttactta acgaaacacc taaagcggtt
     3661 actgaacaaa aaacagaaga aaaagctcct gcaacaactg aaactccggt tgcaactaac
     3721 aaaacaccag ttgttgaaac tcaggaagat ggacgtataa aagcatctcc gttagctaaa
     3781 aaaattgcac aggacaaagg cattaatctc gatgcagttg ccggttcagg tcctgaagga
     3841 agaataatta aaaaagatgt agaagaattt gttccacaaa caacggaagt tgctaaagca
     3901 caacaaccaa ttataaatat ccctcaaata gttggcgaag aaggttatga tgaaacacct
     3961 gtttctcaaa tgcgcaaggt aattgcttca cgtttatcgc aaagtaaatt tacagcaccg
     4021 catttttatc tcaccatttc ggtagatatg gataatgcaa ttgctgctcg taaatcgctt
     4081 aatgaagtca gcccgttaaa aatttcattt aacgacctcg ttgtgaaagc ttcagcaatg
     4141 gcgttgcgca gaaatcctgc tgttaattca agttggttgg gtgatactat tcgtacttat
     4201 aagcatattc atattggtgt tgctgttgca atcaacgacg gactagttgt gccggttatt
     4261 cgttttgcag ataataaatc attatcacaa attgctgctg aaacaggtga tttagctgaa
     4321 cgcgcaaaaa acagaaaatt acaacctgcc gattgggaag gaaatacatt tactatctct
     4381 aatttgggca tgatgggaat tgaagaattt accgcaatta ttaatccgcc cgatgcctgt
     4441 attttagcag taggaggcat tcagcaggtt ccggttgtta aaaatggtca ggttgttccg
     4501 ggtaatgtaa tgaagctcac catgagctgc gatcaccgtg tggtggatgg tgcagtaggt
     4561 gctaaatttt tacaaacatt aaaacaatat ctcgaagacc ctgtgcgtat gctcgcgtaa
     4621 ttcgaggttt aaaatatcat cagttggctt cggccaactt tttttttgct gtattcaaac
     4681 gaactttttt aatgtggtat tcattttatt agtatgtcga agaaaacatt ggtaattggt
     4741 gcttcaaata atccggacag gtatgcttat aaagcagtaa aaaaattaac tgcatacggt
     4801 catcctgttt tggcgcttgg caaaaaggaa gaaatgattg atgccattcc ggtggtaaaa
     4861 gaacgtccgc tcgatactga tatcgatacc gtaacattat atgtaaatcc ccaactgcaa
     4921 aaaaattatt acgattatct gctcaacctt catcccaaac gtatcatttt taatcccggt
     4981 accgaaaacg atgaactcgc agaacttgca caacaaaagg gtattcaaac acttgaagcc
     5041 tgcaccttgg ttttgctttc tataggcgat tattaaacca ccatatttat ttagggcgtt
     5101 accctatagt gattaattat ttgcccccgc aaataattaa tctactatag ggtcgggcta
     5161 tccactccaa ctcctcgtcc gccgaaaagg gcggcctgcg gggtttccgt tactatccct
     5221 aacgcagtgt aaggcagtca tgtagtgctt aataagcata acaatttgaa aacgtcgtgc
     5281 tatgtttagc tcgtccgccg caatgaaatg gaggaggatc cctaacgcag tgagtaatag
     5341 ccatgaaatg cttaatacga atatcactat gaaaacatta attcacatga ccataatcca
     5401 actcattttt ttgacaactt aatcacatac aaatcactgt aaacctgatg aatttagatt
     5461 attattgcag tagcaattat tggaaatttt gagcatcaat atatcacata ccgaagaaaa
     5521 ctacctgaaa gccattttca aactttcaga aatgcaaaat ggcaatgtca gcacaaatgc
     5581 tattgccgaa aaattgtcta ccacagctgc ttcggttacc gatatgctta aaaaactggc
     5641 aggtaaaaag ttgttacact acgaaaaata ttatggtgtt tcgctgacag aaaagggaaa
     5701 aaaactagcc aaagatttaa ttcggaaaca tcgcttatgg gaaacatttt tagtggaaaa
     5761 actaaaattc agttgggacg aagtagacga actggcagaa caattagaac atattaactc
     5821 cgaaatacta accgacagaa tcgaccattt tttaggcttc cctaaattcg acccgcatgg
     5881 cgacccaatt ccggataaac atggtaatat tgcttatcat gaagatgtga cgctcgataa
     5941 aatgcaaaaa ggggacgaag gaattattgc aggggttata gaccactcaa ctgaatttct
     6001 tcaatacctc aataaaattc aattgctcat tcacactaaa ataaaagtga aagacgatga
     6061 agaatatgac cagagcaaaa tcatcatcat caataataaa cacgaaattg ttgtttcaca
     6121 aaaagtagcg gccaatttat tggtacgtaa aataaaataa gaattattgc acaataaata
     6181 gtttcaatct acaagatttt taggtagcgg agtttgctta tttgttttta cctgaacatc
     6241 aattaccatt gttttactcc atttgtcgtg ccaaccgtat ttattcacta aaaacgaaat
     6301 gggttctccc ggtattaatc tgcatgcaga cctgaataaa acctgtttta atgaaatgcg
     6361 ttgtaaacta ttttccctgg taacatgtgt gcctgttatg aatttagcaa ttgtgcgtga
     6421 aaaaaatagt tccattgtcg tgtagtatat aaaattaatc acaataaata agcacccgat
     6481 gtctgctatt gcattaattg cacaaccgct tgccactaaa atggttacag ttaacgtgaa
     6541 aatatggtca ataaaataat taaccagccg tttttttagc ggcgcataaa taaccgaagg
     6601 ttgctgtgta ttaccaacta aatatggtgg aggtgtgcgc cccattttgt caatttgaac
     6661 aaaaatattt attgtagttt aatttgcaaa gtcctgctga gggactggcg tttgaagttg
     6721 cttgcgaatg agggtgtaac agaataccac caaaaaaatc acacccaaaa cacttaccat
     6781 tgcacccgaa acagaggcat ttaaccacaa agccatataa tatccaccag tactggagac
     6841 gatgccgaaa atagctgcaa ccaataacat ggtttttaat ttggtagtta ataaatacgc
     6901 actggctccg ggcacaatta aaaatgcaac caccaaaatg gctccaacac tttcaaaaga
     6961 taatacagtg gtaagagaaa ctgctcccat caataaataa tgccacaatg tcaccgaaat
     7021 acccaatgta attgcatagt caggatcaaa ggtagttaga tataatccct tataaccaat
     7081 agtgataaat ccgataatta aaataaataa ggcacctaat aaccaaacag cagttggaat
     7141 accattccat gtttcaaaat tgataaaagt gatatcgcca tacaatacac aatcctggtc
     7201 taaatctact ttatcggcaa aataggtaat tagaataacg cctaatgcga acaaccaggt
     7261 gaaactaata ccaatacttg cgtcttcctg taatttggct tttttatgaa ataattcaat
     7321 taataccgta cacaacatcc caaaagctgc agcaccaatt aacataggca ctgaactcct
     7381 cgacccactt attaaa
//
LOCUS       DAORKW010000128         7383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_314_length_7383_cov_4.647243, whole genome shotgun sequence.
ACCESSION   DAORKW010000128 DAORKW010000000
VERSION     DAORKW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_314_length_7383_cov_4.647243"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..3697)
                     /locus_tag="PLB46_06715"
     CDS             complement(257..3697)
                     /locus_tag="PLB46_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF76244.1"
                     /translation="MKTLYTSLLGIICLNMLNGQIPEQEIILETHSVFAPSIKQMDIN
                     ADGNLEFIYLRSSKIQVCQNTGDSAWQKIYTINESSADIITDFAFGDVNDDGFDDIIY
                     SPYSGIKKIRVLINPTDNSSNWEEIILTEEARTVIGVFDINSDGWNDIIYEDLTTHIN
                     YYKNNSGAFTEAILIYNPTYTLTLFKSADIDLDGDIDIVGHFNSTGTLFFTNQGDGTV
                     VTTIISSDFYQQQLAVDDFDGDGYLDVMLRYSTLVKIALYDVPSGEYDSPISLGSINA
                     NDYDVADYDNDGDADILLTKFTDDFYSNINCLEHLTGTDFAPETLLFDSIMTKNSTMS
                     FFPYNLDDDLVDVFAFINGNTHVFINNDGEYLNESPITPDISFLRIDLIRLADLNNDG
                     TDEIIAVNNDATIAWLHYNQLTDTITASRKFGKIEGSPYPKQIETADCNNDGYPEVVV
                     SYQGIADYMQSSYYFMNDSVGNFTPHYIDAVAFAEIFFVDANEDGKIDLIGREIEYPN
                     ALKYYANTGDTANLFTFIANLVSGYLYDYNMNDIDNDGDQDFLFVNAFSGYIQSLENI
                     TPGAFGPVTDFLDHSCATAINVYSDDTDEDGDLDIYYTCTTDVTYFAENTGGSYIHHN
                     LGVFDNVTEFGPGYNDQFDLNADGFTDLIACTNSTYGTRIKLTSGTPLPDAAMYTATP
                     DYAILTDFDGNGTTDIIGANTYALYVEYDVIFPKPSISAIFPTAEYFVEAGPIDSVLI
                     FATAVPTVDLTVNLFPGAMVDAGAGEGVMVPIYFNADSTALDTQVVYISIPDDLIVED
                     IFINNISINSDDPSWGIYEGTFELDEPMLITDNDLGIFTSVNEITTTEGAPAVSYGFH
                     LNMVTAAPVNALTTTTDVVLLGGEDTISIEAGIASTYTHANSIFTLSDVYPEENTTGY
                     IIMKLISDDPFINEYEDTVIIVHVNDNDIADIFQTGNPTTKFTEGLDAFTTAFRPLTG
                     FYNDITITAIPDMELDLGAGAGVPVVSVFPASDIIPSDITLEGFPVEDYTFEGIHYGY
                     INFIVSTADVFYDGILMNTIQIKIDDNYDLLSVDDVIENAISIYPQSGNGNFTINWSQ
                     DADVQEIIIVNAIGQHIWNEQINGKLSLDVNLSAPQGVYQVVLLGSTHQFLKQYLLIK
                     "
     gene            complement(3857..4945)
                     /gene="dprA"
                     /locus_tag="PLB46_06720"
     CDS             complement(3857..4945)
                     /gene="dprA"
                     /locus_tag="PLB46_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRF76245.1"
                     /translation="MYHFIALTLIPGVGPVNAKSLLGYCGSAEAIFKTKKRELLKIPG
                     IGEAAAEAIIKKTPFERVDEEISFLEKSGVRCITFIDEDYPKRLKHIHDAPLVLYYSG
                     NGNLNSAKTLGIVGTRNATEYGRHICEQLIEDLKEEQIVFISGLAYGIDIAAHKACVK
                     RDVPNIGVLAHGLDMVYPYAHKDTAKKMIINGGLLTEHMSKTLPDKQNFPKRNRIVAG
                     MVDGLIVVETAENGGAVITAMLANAYNKDVMAFPGNITSPVSKGCNALIKTNRAAIVE
                     NAKDVLDVMCWSKAGEKKQVVQTSMFNELNEDEQLLYNIIKSSPEIGIDEITSKSALT
                     PGTIAGVLLNLEFYSLITNLPGKRYKLL"
     gene            5006..>7383
                     /locus_tag="PLB46_06725"
     CDS             5006..>7383
                     /locus_tag="PLB46_06725"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRF76246.1"
                     /translation="MRYLQIIFIAICCVSGFVSVNAQIIPDHFSRPAYSYRSEQNPYY
                     WKNKKPFDGYWQQDVEYAIKAAIDEKTDIVDGKVVLTYYNNSPDALPFVYFHLYQEAF
                     QPDTYVEELNFSNDVRPTYGKYEGAGLGTEVTEVKIIKRNGKKADEKTILVQDISVLR
                     VNLEKPIQPGENITIEIPFKTYFDTGSLRRRMKKFKSGDFMHYDGVHWYPRICVYDRK
                     MGWDTDQHLGKEFYGDFGSFEVELTFASNYIVEATGYLENEQDMMPRSLREKLDIKNF
                     ANKPWEEKASVIIPYVEGEKKTWKYYAINVHDFAFTADPNYRIGESMAFVNGQQVKCV
                     ALVQEGHAAFWQNAADYTKKIIETFSRDFTPYIWPKIIVADARDGMEYPMLTLDGGYD
                     PSYRDLLIHEVGHMWYFGMVGNNETYRASLDEGFTQFLTAWGYRSIDGDYRVQFPEDS
                     KYKAKYRKPDEIMMSEVYYAYINDAARNTETPLNTHSDMFNSALGHGGGYRQVYYKTA
                     VMLYNLQYVLGDDLFLKAMQNYTAEWKLCHPYFEDFRNSIINYTNVDLNWFFDQWLET
                     TKTIDYGIKSVNKTGTTNLDGQTLNNYDVTFKREGAMQMPIDFSVILENGDTLKYYIP
                     NTWFNKNETVGNNPQGRLDRTYFENVISLPKWYGWDKLNETYVAQITTTQKIKDVIID
                     PSYRLADVDLLDNSWKCPVEWSFDSKVANYNDWKNYTMNWRPEIWGNAYDGLKLGAHF
                     NGDYFGYKHKLEFTAWYNTGLGQGLLYEPEFIVSDDNGDYDVSDDYNMFNFD"
BASE COUNT         2353 a         1266 c         1405 g         2359 t
ORIGIN      
        1 gtaaggttcc agtaggctgt ggctttgtga tacaggccta tactgctgag tgtggccgta
       61 gggtttttta taccttgttc tttcatagta cttaaatagt agttatttcg acgcaaagat
      121 acggcagtga tttttgggaa agaaataact taagtcatcg tttttgtgca taaaaaagcg
      181 ggactttctg gtaaaatccc gcttttgata aatgggcgct gttttaacag taaagccctt
      241 taatctgcaa atgaagttat ttaatgagga ggtattgttt taaaaattga tgtgttgaac
      301 ccaacaaaac aacttgatat acaccttgag gtgcagataa attgacatcc aaacttagct
      361 tgccgtttat ctgttcattc caaatatgtt ggccaattgc atttacaata atgatttcct
      421 gcacatcagc atcttgtgac cagttgatgg taaagttgcc gtttcctgat tgtggataga
      481 tggaaatggc attttcaatt acatcatcta cacttaataa atcataatta tcatcaattt
      541 taatttgaat agtattcatc aaaatgccgt cataaaaaac atccgctgtt gagacaataa
      601 aattaatata accataatga attccttcaa atgtgtaatc ttcaacaggg aaaccttcaa
      661 gggttatatc agatggaatt atatcagatg caggaaatac acttaccacg ggtacacctg
      721 ctccggcgcc caggtcaagt tccatatccg gaattgcggt aatggtgata tcattataaa
      781 atcctgttaa tggtcgaaat gctgttgtaa acgcatcgag tccttcagtg aattttgttg
      841 ttggattgcc tgtttgaaaa atatcggcaa tatcattatc atttacatgg acaataatta
      901 cggtatcttc atattcattt atgaaggggt catcagaaat gagtttcatg ataatatagc
      961 ccgtagtatt ttcttcggga taaacatcag ataaagtgaa aattgaattt gcatgtgtat
     1021 aggttgatgc aattcctgct tcaatcgata tagtatcttc accacctaac aaaacaacat
     1081 ctgtagtagt ggtgagtgca tttacaggag cagctgttac catattcaaa tggaaaccat
     1141 acgaaacagc aggagctcct tcagttgttg taatttcatt tacagaagta aaaatgccta
     1201 aatcattatc ggtaattaac ataggttcat ctaattcaaa tgtaccttca taaattcccc
     1261 atgaagggtc atcggaatta atcgaaatgt tattgataaa aatatcttca actattaaat
     1321 catcaggaat tgaaatatat actacctgag tatccaaagc agttgagtcg gcattaaaat
     1381 atataggaac cattacacct tctccggcac cagcatctac catagctccc ggaaatagat
     1441 taacagttaa gtctactgtt ggaactgctg ttgcaaaaat cagcacggaa tctattggtc
     1501 ctgcttcaac aaaatattct gcagtcggga aaattgcact tattgatggt ttaggaaaaa
     1561 ttacatcata ttcaacatat aatgcatagg tatttgctcc aataatatct gttgttccat
     1621 tcccgtcaaa atcggttaat attgcataat ccggagttgc agtatacatt gccgcatctg
     1681 ggagtggtgt gccggaagtt aatttaattc tagttccata agtggaattg gtgcaggcaa
     1741 ttaaatcagt gaatccatct gcatttaaat caaattggtc gttatagcct ggaccaaatt
     1801 cagttacatt gtcaaacacg ccaaggttat ggtgtatata acttccaccg gtattttcgg
     1861 caaagtaagt aacatcagta gtacaggtat aataaatatc taaatcacca tcttcatcgg
     1921 tatcgtcgga atatacgtta atggcagttg cacaactgtg gtctaaaaaa tcggtaacag
     1981 ggccaaatgc tcccggagta atattttcca gcgattgaat ataaccggaa aatgcattga
     2041 caaaaagaaa atcctgatca ccatcgttgt caatgtcgtt catattataa tcatataagt
     2101 atcctgaaac gagatttgca ataaatgtaa atagattggc tgtatcacct gtgttcgcat
     2161 aatattttaa agcatttgga tattcaattt ctctgccgat taaatctatt ttgccatctt
     2221 cattggcatc tacaaaaaat atttcagcaa atgcaactgc atcaatataa tgtggtgtga
     2281 aatttccaac actatcattc atgaaatagt aactcgattg catataatca gcaattccct
     2341 gataagatac cactacttcg gggtaaccgt cattattaca atcggctgtt tctatttgct
     2401 tagggtaggg gctgccttct attttcccaa atttcctgct tgctgttatg gtgtcagtta
     2461 attgattgta atgcaaccat gctatagtag catcgttgtt aacggcaatt atttcatcag
     2521 ttccgtcatt attcaaatca gctaatctga ttaaatcaat acgaagaaaa gatatgtcgg
     2581 gtgtgattgg tgattcattt aaatattcac catcgttatt gataaaaaca tgtgtattac
     2641 cattgataaa tgcaaacaca tctactaaat catcatctaa attataaggg aaaaaagaca
     2701 tggtgctgtt tttcgtcata atggaatcaa acaacaaagt ttcaggtgcg aaatcagtcc
     2761 cggttaaatg ttctaaacaa ttgatattac tgtaaaaatc atcagtaaat tttgtcaata
     2821 aaatatctgc atcgccatca ttatcgtaat cggctacatc ataatcattg gcattaatgg
     2881 agcctaatga aatagggctg tcatactctc ctgaaggcac atcatacaaa gcaattttaa
     2941 ctaatgtgct atatcgcaac ataacatcta aataaccatc tccatcaaaa tcatcaacgg
     3001 ctaactgttg ttggtaaaaa tcagaagata tgattgttgt gacaacagta ccgtcccctt
     3061 gattggtaaa aaataaagta ccggttgaat taaaatggcc aacaatatct atatcaccat
     3121 ccaggtcaat atcagcactt ttaaataagg ttaaagtata ggtaggatta tatattaata
     3181 tggcttcagt aaatgctcct gaattgtttt tataataatt gatgtgtgtt gtcaagtctt
     3241 cataaataat atcgttccag ccatcactgt tgatatcaaa aacgccaata accgttctgg
     3301 cttcttcagt taaaattatt tcttcccaat ttgaactgtt gtcggttgga tttattaata
     3361 cacgtatttt ttttatacct gaataggggc tgtaaattat gtcatcaaat ccatcatcgt
     3421 taacatcacc aaaagcaaag tcggtaataa tatcagcgga tgactcattg atagtatata
     3481 ttttttgcca ggcactatcg ccggtgtttt gacaaacttg gattttactt gaacggagat
     3541 aaataaattc caaattgcca tctgcattaa tatccatttg tttaatggat ggtgcaaata
     3601 ctgaatgtgt ttctagaata atttcctgct ccggtatttg tccgttaagc atattcagac
     3661 aaataattcc caacagtgag gtgtatagtg ttttcataaa attggttaac aggttatatg
     3721 ttacaaggtg caagttggca actgtagaag gactgcacaa cccacttcgc ttgaaatggc
     3781 ttgtttaacg tatgaaatgc acaaataact tagtgaagca attttgcctt gtagcgtatg
     3841 attaagtttc gatggtttaa agcagcttat atcgtttgcc gggcaaattg gttatcaggc
     3901 tgtaaaactc cagatttaat agcacaccgg ctattgttcc tggtgttaag gcagatttgc
     3961 tggtaatttc atcgatgcca atttcagggc ttgatttgat gatattatac aatagttgtt
     4021 catcctcgtt taattcatta aacatggaag tttgaactac ttgttttttt tcacctgctt
     4081 tcgaccaaca catgacatcg agcacatctt ttgcattttc cactattgcg gcgcgattgg
     4141 ttttaataag tgcattgcaa ccttttgaaa ccggactggt aatattgccg ggaaatgcca
     4201 ttacatcttt attataagca tttgcaagca tagctgtaat tacagcaccg ccattttcgg
     4261 ccgtttcaac cactatgagg ccatctacca taccggctac tatacgattg cgtttgggga
     4321 aattttgttt atcgggaagt gttttggaca tatgctccgt taataatcca ccatttatta
     4381 tcattttttt ggcagtatcc ttatgcgcgt aggggtaaac catgtcgagc ccgtgtgcta
     4441 aaacaccaat attgggaaca tcgcgtttaa cacaagcctt gtgcgcagca atgtcgatac
     4501 catatgccaa accgctgata aaaacaattt gctcttcttt taaatcttca atcagttgct
     4561 cacaaatgtg acgaccatat tcagtggcat ttcgagtgcc gacaatacct aacgttttgg
     4621 cgctgttcaa gttgccattt cccgaataat ataataccag tggagcatca tgaatatgtt
     4681 tgagtcgttt cggataatct tcgtcgataa atgttataca gcgcactccc gatttttcaa
     4741 gaaaggaaat ttcttcatca acacgctcaa atggtgtttt tttgatgata gcctctgcag
     4801 cagcctctcc aattccggga atttttaaca gttcccgctt tttcgtttta aaaatggctt
     4861 cagcactacc gcaatagcct aggagtgatt ttgcattaac agggccaaca cctggaataa
     4921 gcgttaaggc gataaaatga tacatgttta ttaagggcag ggttatatat ttgcgtcaac
     4981 aatttttcac aaatcaagca agaacatgcg ttatctgcaa atcattttta ttgctatctg
     5041 ttgcgtaagc ggctttgtgt cggtaaatgc tcagattatt cctgatcatt tttctcggcc
     5101 tgcgtatagt taccggagcg aacaaaatcc ttattactgg aaaaacaaaa aaccatttga
     5161 tgggtattgg caacaagatg tggaatatgc cataaaagca gctattgacg aaaaaactga
     5221 tattgtggat ggaaaagttg ttttaaccta ttataataat tcccctgatg cgctgccatt
     5281 tgtgtatttc catttgtatc aggaagcatt tcaacctgat acctatgtag aggaattaaa
     5341 tttttcgaac gatgtaaggc caacatatgg caaatatgaa ggtgccggtt taggaacaga
     5401 agtaacggaa gtgaaaataa ttaaacgtaa tggtaaaaag gcggatgaaa aaacgatact
     5461 ggttcaggat atttctgttt tacgtgtcaa tttagaaaaa cctattcagc ccggagaaaa
     5521 tatcacgatt gaaattccat ttaaaacgta ttttgatacc ggttctttgc gcagaagaat
     5581 gaaaaaattt aaatccggtg attttatgca ttatgatggt gtgcattggt atccgcgtat
     5641 ttgtgtgtat gacaggaaaa tgggttggga tacagaccaa catttaggta aagaatttta
     5701 tggtgacttt ggttcatttg aagtagaact cacatttgct tctaattata ttgttgaagc
     5761 aaccggttat ttagaaaacg aacaggatat gatgccgcgt tcgttgcgtg aaaaactgga
     5821 tataaaaaac tttgccaata aaccttggga agaaaaagct tctgtaataa ttccttatgt
     5881 tgaaggcgag aaaaaaacat ggaaatatta tgctatcaat gtgcatgatt ttgcatttac
     5941 ggccgacccg aattaccgga taggagaaag tatggcattt gtaaacggac aacaggttaa
     6001 atgtgttgcg ttagtgcagg aaggacatgc agcgttttgg caaaatgccg ctgattatac
     6061 caaaaaaata atcgaaacat ttagtcgtga ttttacgcct tatatctggc cgaaaataat
     6121 tgttgcagac gctcgcgacg gtatggagta tccgatgtta actttggatg gaggatatga
     6181 cccttcttat cgcgatttat taattcacga agtgggacat atgtggtatt tcggtatggt
     6241 gggcaataat gaaacatacc gcgcttcatt ggatgaagga tttacccaat tcttaactgc
     6301 ttggggttat cgcagtattg atggcgatta tcgtgttcaa tttccggagg atagtaaata
     6361 caaagcgaaa tatcgcaagc ccgatgaaat aatgatgagc gaagtgtatt atgcctatat
     6421 taatgatgca gcccgtaata ctgaaacacc gcttaataca cattccgata tgtttaattc
     6481 agcgcttgga catggtggcg gttaccgaca agtgtattat aaaactgcag taatgttgta
     6541 taatttacaa tatgtattag gagatgattt gtttttgaaa gccatgcaaa attataccgc
     6601 tgagtggaag ttatgtcatc catattttga ggattttaga aattcgatta tcaattatac
     6661 caatgtagat ttaaactggt tttttgatca gtggttagaa acaacaaaaa ctattgacta
     6721 cggaattaaa tccgttaata aaacaggaac cacaaatctc gatggtcaaa cattaaataa
     6781 ttacgatgtc acctttaaac gtgaaggtgc tatgcaaatg ccaattgatt ttagtgtaat
     6841 attagaaaat ggtgatacac ttaaatacta tattcccaat acctggttta ataaaaatga
     6901 aacggtgggt aataatcctc aaggcagact cgacagaacc tattttgaaa atgtaataag
     6961 tctgccaaaa tggtacggtt gggataaatt aaatgaaaca tatgttgctc aaattaccac
     7021 aacacaaaaa attaaagatg tcatcattga tccgtcttat cgtttggctg atgtggattt
     7081 gctggataat tcgtggaaat gtccggtaga atggagtttt gatagtaaag ttgcaaatta
     7141 taatgactgg aaaaattata ctatgaactg gcgccctgaa atttggggaa atgcttatga
     7201 tgggttgaaa ttaggcgctc attttaatgg cgattatttt gggtataaac ataaattaga
     7261 atttactgcc tggtataata ccggtttggg ccagggttta ttatatgagc ctgaatttat
     7321 tgtttcggat gacaacggcg attatgatgt atcagatgat tataatatgt tcaattttga
     7381 ttt
//
LOCUS       DAORKW010000129         7355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_317_length_7355_cov_4.907534, whole genome shotgun sequence.
ACCESSION   DAORKW010000129 DAORKW010000000
VERSION     DAORKW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_317_length_7355_cov_4.907534"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PLB46_06730"
     CDS             <1..742
                     /locus_tag="PLB46_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF76247.1"
                     /translation="FIAFSQTTYCQVTFNYGPEIGVTTPHLPALQNTNPQGEAITETY
                     FPIISPIIGFQSQATFCQHLQLTAGLQYQITGTNFHSHSDGSDIRDDGSGTYYTYTYD
                     IWEEQRFHKITVPVTVGYKFTIGHFTPTIYTGLRPNLFLTGTYAYNTEYVATDNAEPG
                     ASENEFNPLSKLEARTPMAHCNFQFTGGVSTEIGPRIKIAINYSRGPEIFYAEKSTSK
                     ESDYINMINQDLSLSLTYLLADIVKVAK"
     gene            913..1836
                     /locus_tag="PLB46_06735"
     CDS             913..1836
                     /locus_tag="PLB46_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRF76248.1"
                     /translation="MQKTTIAFLVLPHVHLLDLAGPDQVFLEAIGYNAPFEIVYCSIG
                     DDVASSALLPFGKLKHFSKIKLKPQDFLIVPGAELSYLQSKHFKNNTNLFTWLTSCYA
                     NHINICSICSGAFVLAESGLLNGKECTTHWKRTKELQQLYPKAKVVENVLFTEDGNIY
                     ASAGIASGIDMALYIVEKIMGSFFAHKVARELVIYTRRSGNQHQHSELLNYRNHIHTG
                     IHKTQDWLHENLHKKVSLKFLAGIANMSDRNFTRVFKKETSLTVNEYITLLRKEKITQ
                     LLNNPDMSRAQIAKQCGLKSERQVSRLINNK"
     gene            1869..4436
                     /locus_tag="PLB46_06740"
     CDS             1869..4436
                     /locus_tag="PLB46_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRF76249.1"
                     /translation="MLHKLRLVVVLSAVLTGITEIIAQTPNSAPQQIDIIHQDIELSF
                     DWEKQQAYGSTALYMYILQPTSTISLAAQQLTIQNIYANNSQQLLFTENIGIVVQLDR
                     TYAAGDSLKLKIDYHTNWVNTSDPNFLSGSNGQGLRFFQPTKTEPTKKRQIWSACDYG
                     SLPLWLPYINNTEDLRTTSFTTTIDSSLTLITNGHLVKATTKGSGTTYHYFMNHPYSA
                     DLTAFVIGEYSDVRQYAGNIQLHNYSYKDEVAATNASVERLPDMVDYFSKTTGVPYPY
                     ETYSQVFVLDVPWGMAATSLAIQTENMVDDFDTHADFLYLWDGLEGESLAQQWFGNYV
                     TCKDPNHIWLTKGIARYFSGLYNANKNGKDEFLLWQHAYDQSTYFFDWDYGVRQALVN
                     TDTNNIYNFINSNTPYFHAAAFMRMLHKELGETQFKSVIQEYLTTYASTSVTTENLIT
                     TIEKVTGKPITWFFDQWVYQTGHPIFDVQQAYDAKNKLFTLQVVQTQTFDSLTAYKQT
                     PYFKGNMEIAINNKIHQINIEPKKENIFIFSMEEKPKLVNFDYESTWLKQVTFEKSLE
                     DLIYQLQFDSDVLGRQSALNALTTIAQNDTTSLSTKENILAQYREIILSDTYWRFKLI
                     TLTQLQNILVPWGSTDPINLDESTTNMLLELIKKDSSWVCAAAIRFLGLTKNVAYADI
                     FINALKNTSDRVVNSAAIALGKTKSEKAYDALAKLLGRPSWKNQSLMSALSGLKELGD
                     PRGYNIAYNALQDVYLSRWYLPTPPVWDFRVVAVETIVALNRGSDILPLLLTRFENAL
                     KEGDINTVFNYVVLLSKIPDEKVKPVFEKLKIVYQNDSNTLIAVNTFEQQYLTLIQK"
     gene            4439..4930
                     /locus_tag="PLB46_06745"
     CDS             4439..4930
                     /locus_tag="PLB46_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76250.1"
                     /translation="MEIIASATSNEHDFDFLVGKWQVKNRKLVARLANCTDWITFDSE
                     IHMVKTLNGFGNMERYFATFDNQPFEGMAIRLFNPETKLWKVYWTDSSSCKMDEHPVT
                     GSFENGLGKFYAADFFNGIPIVVLYQWDASNKQFPKWSQAFSIDNGLTWEWNWEMQLE
                     KLE"
     gene            5032..5673
                     /locus_tag="PLB46_06750"
     CDS             5032..5673
                     /locus_tag="PLB46_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HRF76251.1"
                     /translation="MTTHKKVCIFGTGGFGRETFCCLLDAMKVKDFNAAASTCFMVGD
                     EHYTTSEVMGIEVIRQSAFQPEIYDVVVAIGDPAARKKVVESLPPQTTYTTIVHPSVI
                     MSQWVTIGEGSIITAGTILTCNISIGKHAQLNLHTTIGHDCVIGDYFTTAPAANISGN
                     CTFGEQVYIGTNVAVKQGVTIPSETTIGMGAVVVKDIATAGIYIGNPLKQLIK"
     gene            5699..6103
                     /locus_tag="PLB46_06755"
     CDS             5699..6103
                     /locus_tag="PLB46_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF76252.1"
                     /translation="MTITVSTTVNAPISKVWDYWNLPEHIKQWCSASEDWHVPDAEND
                     LREGGEFTTTMAARDGSFSFDFNGIYTNIIEHQLIEYRMSDGRKVSITFSEKNNAVEI
                     IESFEAETENSEEMQQAGWQAILDSFKAYAEK"
     gene            6726..>7355
                     /locus_tag="PLB46_06760"
     CDS             6726..>7355
                     /locus_tag="PLB46_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF76253.1"
                     /translation="MKVEQIYTGCLAQGAYYIESNGEAAIIDPLREVQPYIDMAKTRG
                     SKIKYIFETHFHADFVSGHVDLSKKTGAQIVYGPTKMKTGFEALIGEDGQVFKLGNAT
                     IKLLHTPGHTMESSCFLLSDETGKETAIFTGDTLFIGDVGRPDLAQHVIADLTQEKLA
                     AHLYESLQNKILPLSDDLIVYPAHGAGSACGKNLSKETSDTLGNQKKTNS"
BASE COUNT         2476 a         1274 c         1321 g         2284 t
ORIGIN      
        1 atttattgcc ttttcccaaa caacctattg ccaggttacc tttaattatg gccctgaaat
       61 cggtgttaca actccacatt tacccgcact tcaaaacaca aatcctcagg gtgaagcaat
      121 aactgaaact tattttccta ttataagtcc cataatcggg tttcaaagtc aggctacttt
      181 ttgccaacat ctgcaactaa cagcgggtct tcaataccaa ataacaggta ccaattttca
      241 ttcacatagt gatggttcag atatacgcga tgatggcagt ggaacttatt atacctatac
      301 ttatgacatt tgggaagaac aacgttttca taaaataact gtaccggtaa ctgttggata
      361 taaatttact attggtcatt ttacgccaac aatttataca ggtttacgtc ctaatttatt
      421 tttaaccggc acttatgctt ataataccga atatgtagca accgataatg ccgaaccggg
      481 tgccagcgaa aatgaattta accccttaag taaattagaa gccagaactc caatggcgca
      541 ttgtaacttc caatttacag gtggtgtgtc aactgaaatt ggccctagaa ttaaaattgc
      601 aattaattat agcagaggtc ctgaaatatt ttatgcagaa aaaagcactt ctaaagaatc
      661 cgattatatt aatatgatta atcaggattt aagccttagt ttaacttacc tgttggctga
      721 tattgttaaa gtagccaaat aattttaatg ttattaccgt aggtttttac atttcccaat
      781 atttcctatc ttggtgccgg gttaagtatt aatgagcaaa agctcctttt ataccgtggc
      841 tcaaaatgtc cgttttttgg ctgcacaagt gattgaatta ccggtaggtt tgtagcttac
      901 taatttcatg ttatgcaaaa aactacaatt gcatttttag tattaccaca tgtgcacctg
      961 ctcgacctgg caggtcccga tcaggttttt ttagaagcta taggatataa tgcgcctttt
     1021 gaaattgtat attgttcaat tggtgatgat gttgcttcct ccgccttatt accttttggc
     1081 aaactgaaac atttttccaa aataaaatta aaacctcagg attttttaat agtcccgggt
     1141 gccgaattaa gttatttaca atccaaacat ttcaaaaata atacgaacct gtttacctgg
     1201 ttaactagtt gttatgccaa ccacataaat atttgcagta tttgcagcgg tgcatttgta
     1261 ttagctgaaa gtggcttgtt aaatggcaaa gaatgtacta cacattggaa acgaacaaaa
     1321 gaacttcaac aattgtatcc aaaagccaaa gtagtagaaa atgttttatt tactgaagat
     1381 ggaaatattt atgctagcgc aggcattgct tcaggtatcg acatggcatt atatattgtt
     1441 gaaaaaataa tgggcagttt ttttgcgcac aaggttgccc gcgaattagt tatttacacc
     1501 cgccgttccg gtaatcaaca tcagcatagc gaattattaa attatcgcaa tcatatacat
     1561 accggtattc ataaaacgca ggattggtta cacgaaaatt tacataaaaa agtatcgctt
     1621 aaatttttag caggaattgc caatatgagc gaccgtaatt ttacacgtgt tttcaaaaaa
     1681 gaaacttcac tcactgtgaa cgaatacata acattattgc gcaaggaaaa aattacgcaa
     1741 ttattaaata accccgatat gtcgcgcgcc caaattgcaa aacaatgtgg ccttaaaagc
     1801 gaacgccagg ttagtcgcct gattaacaac aagtaaatcc taatttttta atcacttaac
     1861 ttatttttat gttacacaaa ttacgtttag tagttgtgtt gtcagcagtg ttaacaggca
     1921 ttacagaaat aattgcccaa acaccaaact cggcacctca gcaaattgat attatacatc
     1981 aagatattga attatcgttt gactgggaga aacaacaagc ttatgggtca acagctttgt
     2041 atatgtacat actgcaacct acatcaacaa tttcattagc cgcacaacaa ctcactattc
     2101 aaaatatata tgcgaataat agccaacaat tactttttac tgaaaatatt gggatagtag
     2161 ttcaacttga tagaacctat gctgcaggcg attcgctaaa attaaaaatt gattatcata
     2221 ccaattgggt aaacacaagt gacccgaatt ttttaagcgg aagtaacgga cagggtttac
     2281 gtttttttca accaacaaaa acagaaccaa ctaaaaaacg acaaatttgg tctgcctgcg
     2341 attatggttc tttacccctg tggttgcctt atatcaataa tacggaagac ttaagaacaa
     2401 catcatttac aacaaccatt gacagttcat taaccttaat tacgaatgga catttagtga
     2461 aagcaacaac aaaaggtagc ggcaccacct atcattattt tatgaaccat ccttattcag
     2521 ctgatttaac tgcatttgta attggagaat atagtgatgt gcggcaatat gcaggaaata
     2581 tccaattgca taattacagc tataaagatg aggttgcagc aactaatgca agtgttgagc
     2641 gattacccga catggtagat tatttttcca aaacaacagg cgttccgtat ccttatgaaa
     2701 cttatagtca ggtatttgtt ttggatgtac cctggggtat ggcagcaaca tcattggcca
     2761 tacaaacaga aaatatggtg gatgattttg acacacatgc cgatttctta tatctatggg
     2821 acggactgga aggcgagtcg cttgcgcaac aatggtttgg caattatgtt acttgtaaag
     2881 accccaatca catatggtta actaaaggta tagcccgata tttttccggt ttgtataatg
     2941 ctaataaaaa tggaaaagat gaatttctat tatggcaaca tgcatacgac caaagtacgt
     3001 attttttcga ttgggattat ggggttcggc aagcattagt aaatactgat acgaataaca
     3061 tctataattt tattaacagc aatacgccat attttcatgc agcagctttt atgcgaatgt
     3121 tgcataagga attaggtgaa acacaattta aatcggttat acaggaatat ttaactactt
     3181 acgccagcac ttcggttacc actgaaaatt taataaccac aattgaaaaa gtcacaggta
     3241 aacccataac atggtttttt gatcaatggg tgtaccaaac aggacaccct atttttgatg
     3301 tgcagcaagc gtatgatgct aaaaataaat tatttacatt gcaagtagtg caaactcaaa
     3361 cttttgatag cttaaccgcg tataaacaaa caccatattt taaggggaat atggaaattg
     3421 cgatcaacaa taaaatacat caaataaata ttgaaccaaa aaaagaaaac atatttatat
     3481 tttcaatgga ggaaaaacca aaactggtaa atttcgatta tgaaagtaca tggctaaaac
     3541 aggtgacttt cgaaaaatca ctagaagatt taatttatca attacaattc gacagcgatg
     3601 tattgggtcg gcaatctgca ttaaatgcat taacaacaat tgcacaaaac gatactacat
     3661 ctctttctac taaagaaaat attttagccc aatacaggga gattatttta agcgatacct
     3721 attggcgatt taaattaatt acgttaacac aattgcaaaa cattttagtt ccctggggta
     3781 gcactgaccc gataaattta gatgagtcaa caacaaatat gttgttagaa ttaatcaaaa
     3841 aggatagttc ctgggtttgt gctgcagcaa ttcgtttttt agggttaaca aaaaatgttg
     3901 cttatgccga tatttttatc aacgcattaa aaaacacaag cgatagggtt gtaaattctg
     3961 cagcaattgc attaggtaaa acaaaaagtg aaaaggctta tgatgcgctt gccaaactac
     4021 ttggcagacc ttcatggaaa aaccaaagtt taatgagtgc cctttcagga ttaaaagaat
     4081 taggtgaccc tagagggtat aatattgctt ataatgcgct tcaggatgtt tatttaagca
     4141 gatggtattt accaacaccg ccggtttggg atttccgggt tgttgctgta gaaacaattg
     4201 ttgcgctcaa cagaggtagt gatatattac cattattact tacccgtttc gaaaatgcct
     4261 tgaaggaagg tgatattaat accgttttta actatgtagt attattatca aaaattcccg
     4321 acgaaaaagt aaaaccggtt tttgaaaaat tgaaaatagt ttatcaaaat gacagcaata
     4381 ctttaatagc tgttaacacc tttgaacaac aatatttaac cttaatacaa aaataactat
     4441 ggaaataatt gcttcagcta catcaaacga acatgatttt gactttttag ttggaaaatg
     4501 gcaggtgaaa aacagaaaac ttgttgcacg tttggccaat tgcaccgatt ggataacatt
     4561 tgattcagaa atacatatgg ttaaaaccct gaatggtttt ggcaatatgg aacgttactt
     4621 tgcaaccttt gataatcagc catttgaagg catggcaatt cgtttattta atcccgaaac
     4681 aaaattatgg aaagtatatt ggaccgattc gtccagttgc aaaatggatg aacatcctgt
     4741 tactggttca tttgaaaatg gtttaggcaa attttatgca gcggattttt ttaatgggat
     4801 acccatagtg gtattatatc aatgggatgc aagcaataaa caattcccta aatggtcaca
     4861 agcattttca attgataatg gcttaacttg ggaatggaac tgggaaatgc aattggaaaa
     4921 attggaataa aatagcattt caaaggttaa tgcattggca ataatttacc ttttgtttaa
     4981 ggaactctgc gtgtaaagaa ttatctttga ataacaaaag gaagttcata tatgactacc
     5041 cataaaaagg tttgcatttt tggcacagga ggatttggac gggaaacatt ttgttgtttg
     5101 ctggacgcta tgaaggtgaa agattttaat gctgcagctt ccacttgttt tatggttggc
     5161 gatgaacatt acacaacatc agaagtaatg ggaattgaag taattcgcca atctgcattt
     5221 caacccgaaa tctatgatgt tgtagtcgcc attggtgatc ctgctgccag aaaaaaagta
     5281 gtggaatcac ttccgcctca aacaacatat accactattg ttcatccaag tgttattatg
     5341 tcgcaatggg ttaccattgg tgaaggtagt attattaccg caggcacaat tttaacctgt
     5401 aatatcagta ttggcaaaca cgctcaatta aatttacata ccaccattgg ccacgattgt
     5461 gtgattggcg attattttac tacggctcca gcagctaata tcagcggcaa ctgtacattt
     5521 ggcgaacaag tttatatcgg aaccaatgtt gcagttaaac aaggtgttac tattccatct
     5581 gagaccacta ttggaatggg tgctgttgtt gtaaaagata tcgccactgc cggaatatat
     5641 attggcaacc ctttaaaaca attaattaag taatttatca taaattaaaa taaccgcaat
     5701 gaccattact gtatctacaa ctgttaatgc tcccatctca aaagtttggg attattggaa
     5761 tttacctgaa catattaaac aatggtgttc ggcatcagag gattggcatg tgccggatgc
     5821 agaaaatgat ttgcgcgaag gcggtgaatt taccacaaca atggctgcac gtgatggcag
     5881 tttcagtttc gactttaatg gtatatatac aaatattatt gaacatcaat taattgaata
     5941 tcggatgtct gatggtagaa aggtaagtat tactttcagt gaaaaaaaca atgctgttga
     6001 aattattgaa tcatttgaag ctgaaaccga aaatagtgaa gaaatgcaac aagcaggttg
     6061 gcaggctata ttagatagtt ttaaagcgta tgctgaaaaa taatttaatt aaaaccattc
     6121 aaacctattt tcctgttttc cttttcattt tagttacagc ttacaccatt ataaccagat
     6181 tattaagtgg taataacttt tcttttgaat atcaggtgca tgttgtgtta ctaatttttg
     6241 tcgggatatc atttttattt aaaaagcctg ctagcttatt tacaacattg gtagttctgg
     6301 ttatagggtt attttttcct tccgtttttg ttataggcaa caaaacatat tattactcct
     6361 tttccataag ttttctgcat ctaaatggcc tttattttat attgttgttg ttgtttatta
     6421 tttgtaatta caaattaata cccggcttaa ttcaatgggc atttaataac aactccgcat
     6481 caaataatta aaataagtag caaagagcag gcttacgagg cttggaactt tttgattata
     6541 ggctatgttt tcttcttttt gatgattttt gtcagctttt ttgatgcatg accaacttaa
     6601 ctttgtggta aatctgacag cggcaatgcc ggaaggcaga ttatgcaaaa taacagctct
     6661 gtaacaaaag taaccgaaat aactatttca ctatatcatc tttgcatttt aaataattaa
     6721 ctcatatgaa agttgaacaa atttataccg gctgccttgc acagggagcc tactatattg
     6781 aaagtaacgg cgaagcagcc ataattgacc cattacgcga agtacaacca tatatcgata
     6841 tggcaaaaac acgtggaagt aaaatcaaat atatttttga aacccatttt cacgccgatt
     6901 ttgtttcggg tcatgtggac ttgtccaaaa aaaccggagc acaaattgta tatggtccta
     6961 ccaaaatgaa aaccggattt gaggcattga ttggtgaaga cggacaagta ttcaagttag
     7021 gtaatgctac cattaaatta ctgcatactc ccggtcatac tatggaaagc agttgttttt
     7081 tattaagtga tgaaacaggt aaagaaaccg ccatatttac aggtgacact ttatttattg
     7141 gcgatgttgg ccgccccgat ttagctcagc atgttattgc ggacctcacg caggaaaaac
     7201 tggcagcaca tttatacgaa tcgctgcaaa ataaaatatt gcctttaagt gatgacctta
     7261 ttgtatatcc tgcgcacggt gcaggtagtg cttgtggtaa aaacctaagc aaagaaactt
     7321 ccgacacctt aggcaatcag aaaaaaacaa atagc
//
LOCUS       DAORKW010000130         7319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_318_length_7319_cov_5.272990, whole genome shotgun sequence.
ACCESSION   DAORKW010000130 DAORKW010000000
VERSION     DAORKW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_318_length_7319_cov_5.272990"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>830)
                     /locus_tag="PLB46_06765"
     CDS             complement(<1..>830)
                     /locus_tag="PLB46_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HRF76254.1"
                     /translation="IIDPHGDVAKYLLEHIPENRKKDLVYINPVDENVIAFNPLHAVH
                     PNYHSLVASGLISTFKKIWADSWGPRMEYILRFSLLTLLQYPYATLLDIQPLLTDKHF
                     KEKVLEYLKDDYIFDFWRKEFDTYTPSFRNEVISPILNKMGVFISSKPLRNMLGKQVS
                     GFRMSQVMDEQKILIANLSKGELGEDASTIIGSILVTSIQLSALYRAKQPMEKRIPFY
                     LYVDEAHSFLSLSFADILAESRKYGLSLFLAHQYIEQFHEDIRSSIFGNVGTLISFRV
                     G"
     assembly_gap    831..840
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1165..1290
                     /locus_tag="PLB46_06770"
     CDS             1165..1290
                     /locus_tag="PLB46_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76255.1"
                     /translation="MIEEQNRQSNMSEDDAIDYIAQLLVEMFLDKKHDEQFRNNN"
     gene            1265..2890
                     /locus_tag="PLB46_06775"
     CDS             1265..2890
                     /locus_tag="PLB46_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRF76256.1"
                     /translation="MNNLETTTKKRAVIYCRVSTDKQAVNYSLETQEKICCDYADRNN
                     YEVVEKFVEEGESAKTADRTKLKELLRYCYDKTNTISVVIFYKLDRFARQRMDYDVVC
                     SGLRAKGLIIRSATEPIEETPTGRLMEGMLASYAQFDNEIRTERSVLGMRAALRAGRF
                     IWGAPIGYNNIKIEDKGTITMSDKAPIVRVAFEEVAKNKEPAEVIYKSMIKIGLTNRK
                     GKPLARSQFYEMLKNPLYAGVVQGFGESHEASFEPIVSKDLFDRVQFVLKYRSRKNYT
                     AYQIQNPDFPLRRFIAHPEGVKMTGSWSKGRTKKYAYYRFPSKGIEISKDKLEGAFTE
                     ILNQFQFEKVIMTRFNASIKKHFQSATASQRNILQAIPKLIEEQEAKLDLAFLEKQKG
                     TISEFIFQRQTQTIEKRLGELQEQLGQKTETKYDFNEMMDYVAEYLKNPSKIWVEAKF
                     EKKLKLQWFQFPKGILFDGEKFKTEEIRSIYKVKDIFLPCKSDGVPPADHTSNGGKIC
                     YLQNLNPSSISDKEFWEQVKGDLIELKAILESD"
     gene            complement(3035..3592)
                     /locus_tag="PLB46_06780"
     CDS             complement(3035..3592)
                     /locus_tag="PLB46_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76257.1"
                     /translation="MPKITKIVTLKVMRDKIWHQMFQAKHAEIYLRYYLDRKKEIKNR
                     LNVSILFTTVVGAAGWKIELLPVISCLLATILVFIDRYGKEFVLTDEQLKTFSELKSK
                     NIQYCSRLEEMYDKLDRNLITENEALSEFYIARKIYEEIENMDDTANVFTNRKLVEKS
                     RTETLEYFNNYHKLNNNEQARNAEN"
     gene            3637..4521
                     /locus_tag="PLB46_06785"
     CDS             3637..4521
                     /locus_tag="PLB46_06785"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HRF76258.1"
                     /translation="MNSDGKVSLEETLNFINFLTMKSKEKSPESYIAIKNAINIFSIP
                     TQRFLFKKSNFLYRAHNNDKTPRFFSDTRLGANRHPNTIDKFGRANEPGQSVFYCSDN
                     CYVAYMETSHVVRNTDKPLKHKMTIGTWEVVEDLRLATLPSNSTIAGLNPTVDALHKN
                     YMELVEQYRDGTTDSTLVLLDYFSRMFSSEADGDSTNYLISCAIANYYYENLVFDYEL
                     QKAIYLDGIIYPSVMLKTEGVNLALTYDTYNSREPVKLKLVDVVELTMVRVGDKTYEE
                     ETIVKCKSINQETGEILW"
     gene            4614..4895
                     /locus_tag="PLB46_06790"
     CDS             4614..4895
                     /locus_tag="PLB46_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRF76259.1"
                     /translation="MENKGRYIPNNLIKYRKMLGLKQEEVASYLGLKSTNRLSRWEQG
                     LSMPSAQNLFKLSTLYHTLIDQLYSDHVSHIKRNMSENEELLDSSHPKK"
     gene            5297..5779
                     /locus_tag="PLB46_06795"
     CDS             5297..5779
                     /locus_tag="PLB46_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76260.1"
                     /translation="MGGVTILGISPGTRYIGIALQRDGILYDWRIKSYKGVWSEEKLS
                     KVVTYIENLILTHVVEHIACKIPHIGRCSEGLKLLIEKIKSIAEKCNSKIHLYSISEL
                     KELFTSSIQNKGALVNYLAEKLPELRHLLKKELRNKNTYYVKVFEAIATTVHCHNSLK
                     "
     gene            5902..6528
                     /locus_tag="PLB46_06800"
     CDS             5902..6528
                     /locus_tag="PLB46_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76261.1"
                     /translation="MESEYYITAFFGLIGTLIGAGISFFANVYQSSKEAKLRVREKIF
                     DKRINTHEEIIAFCQVMRTTVSTHEGDAQNNVITYPMLLINREHYGNFIKNFVQVLGR
                     NSIWMDIELYRYMNYIQDYLESLSSNIRDIEEKSFPLVGVLIKQDFINLALELEKEAF
                     KFFEKDIYNINLKMKKGHHKFPREETVKKFKNTQLNKNIAAIKNIQIK"
     gene            6550..7182
                     /locus_tag="PLB46_06805"
     CDS             6550..7182
                     /locus_tag="PLB46_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76262.1"
                     /translation="MNTQSILDFIESLGIIVASVTATLGLYAWRKEVKWKRRNEVAEE
                     ILYNLYDVRDRLRAIRHPFAHPSEGETRLVTKLESVEEKTIRNYAYTTVERYNNNREP
                     FLNLEKLKHRYAIYFGHKNASSITNIIFLKNEIISASYKYAQRSIERDKLIKKNLGEG
                     YIQNQINALDTQIEKYASIIWEKYEDDDAISSAIDETIANQEIVLNKYVK"
BASE COUNT         2545 a         1253 c         1346 g         2165 t
ORIGIN      
        1 ccaactcgaa aactaatcag tgttccgacg ttgccgaaaa tcgaagaccg tatatcttcg
       61 tgaaattgct caatatattg gtgagctaga aagagtgaga gtccgtattt ccttgattca
      121 gcgagaatgt ctgcgaaaga aagggaaaga aacgaatgtg cttcatctac atataagtaa
      181 aacggaatac gcttttccat cggttgtttg gctcgatata aagcagaaag ctgaatcgaa
      241 gtaactaaaa tactaccgat aattgtagac gcatcttccc caagttcacc ttttgatagg
      301 ttggcaatga gtattttttg ttcatccata acctgtgaca ttcgaaaacc tgaaacctgt
      361 tttcccagca tatttctcaa aggtttactt gatataaaca cacccatttt gttgagaata
      421 ggagaaatca cctcatttcg gaatgaaggt gtataggtat caaactcttt tcgccagaaa
      481 tcaaaaatgt aatcatcctt taagtattca agcacctttt ctttgaagtg cttgtctgtt
      541 agaaggggtt ggatatcaag caaggtagca tatggatatt ggaggagtgt caggaggcta
      601 aaacggagta tgtattccat tctagggccc caggaatcag cccaaatctt tttaaaggtg
      661 gatatgaggc ctgaggcaac taagctatgg taatttggat gcactgcatg tagtgggtta
      721 aacgcaatca cattttcatc aactggattg atatatacta gatctttttt tctattctcc
      781 ggaatatgtt caagaagata ctttgctaca tctccgtggg gatcgataat nnnnnnnnnn
      841 gcctttgcct ttttgtatgt ctgaaagtgc cattttttgt aataacgtag atttccccat
      901 tccggtcttt ccgatacagt aaatatgccc atgtctgtcc tggtctttaa taccaaaagg
      961 aatgttttgg ttgcgccaat ttgtgtagcc tattggcgta atgtcataat catccatact
     1021 catactggat attgtagaag atcaagttat tcagcataag aggggtaaaa ttgctatttc
     1081 cagcaaattt gaccccctta tatatctcac ctcagtaatt cacaatgtag gggtactcac
     1141 aagaacaaaa aagtacgcta cgctatgatc gaggaacaaa acagacagtc gaatatgtcc
     1201 gaagatgatg cgattgatta tatcgcacag cttttagtag aaatgttcct cgataaaaaa
     1261 cacgatgaac aatttagaaa caacaactaa aaagagggct gtcatctatt gccgcgtttc
     1321 tactgataag caggctgtga actacagtct ggaaactcag gaaaagattt gttgcgatta
     1381 tgcagaccga aacaattatg aggtggtaga aaagttcgta gaggagggag aatctgctaa
     1441 aaccgccgac cgaacgaaat taaaggaact attgcgctat tgttatgaca agaccaatac
     1501 aatttcggtg gttattttct ataagttaga tcgttttgcc cgccagagga tggactatga
     1561 tgttgtgtgc agtgggttaa gagcaaaagg actcattatt cgctctgcaa ctgaacctat
     1621 agaagaaacc ccaacaggaa ggctcatgga gggcatgctt gcctcatatg cccagtttga
     1681 taatgaaata cgcacagaac gctctgtgct aggtatgagg gcagctttac gggctggtag
     1741 gttcatttgg ggtgcaccaa ttggatacaa caacattaaa attgaagaca aaggaacaat
     1801 taccatgagt gacaaagcac ccatagtccg agtggctttt gaagaggttg caaaaaacaa
     1861 agagcctgct gaagtaattt acaaatcaat gattaaaatc gggctaacca acagaaaagg
     1921 aaaaccgctg gctaggtcac aattttatga gatgcttaaa aaccctttgt atgcgggtgt
     1981 tgttcaaggg tttggggagt cgcatgaggc ttcctttgag ccaatagttt caaaagacct
     2041 gtttgatcga gtgcagtttg tactaaaata tcgctctcga aaaaactaca ccgcctatca
     2101 gatccaaaac cctgactttc cattacgccg atttattgca cacccggagg gtgttaaaat
     2161 gaccggctcc tggtctaaag gaaggacaaa gaaatatgcc tattaccgtt ttccatcaaa
     2221 aggaattgag atatcaaagg ataaactaga aggtgctttt acagaaatat tgaatcagtt
     2281 tcagtttgaa aaagttatta tgaccagatt taatgcttct ataaagaagc actttcaaag
     2341 tgcaacggcc tctcaaagga atatactcca ggcaatacct aaactcatag aagaacaaga
     2401 agccaaactt gatttagcct ttttagaaaa acaaaaagga actatcagtg agttcatttt
     2461 tcaaaggcag acacaaacta tcgaaaaacg acttggggaa ttacaagaac aattagggca
     2521 aaaaactgaa actaagtatg atttcaatga gatgatggat tatgttgccg aatatctaaa
     2581 aaacccaagt aaaatatggg tagaagcgaa atttgaaaag aaactaaaac tacaatggtt
     2641 ccaattccca aaaggtatcc tctttgatgg agagaaattt aaaaccgaag aaatacgtag
     2701 tatttacaag gtaaaagata tttttttacc ttgtaaatcc gatggggtgc ccccagccga
     2761 ccacacttcg aacggaggga aaatttgcta cttacaaaat ttaaaccctt caagtatatc
     2821 agataaggaa ttttgggagc aagtaaaagg tgacctaatt gaattaaaag caatattaga
     2881 aagtgattaa ccaatctata ttgcgtttaa ttcaatatac tgttacttct gttcctttgg
     2941 cggaggtggt ggtgcctttt caaatcttgg aactgagccc tttaaatctc cggttttaat
     3001 agttgtaatc gactttggtt gaaccgaaat tttctcaatt ttcggcgttt cttgcttgtt
     3061 cattgttgtt taatttatga tagttattaa aatactctaa tgtctctgtc cttgattttt
     3121 caactagttt tctgttggta aaaacgtttg cagtgtcatc catgttctct atttcctcat
     3181 atatctttct tgcgatataa aattcagata aagcctcgtt ttctgtgatt agattccgat
     3241 ctagtttgtc atacatctct tctagtcttg aacaatactg gatgtttttg cttttcaatt
     3301 ctgaaaaggt ttttagctgt tcatcggtta gcacaaattc tttcccatat ctgtcaataa
     3361 atactagaat tgttgctagt aaacaagata ttacaggtaa caattcaatt ttccagccag
     3421 ccgcgccaac cactgtggta aacagtatag acacattcaa tctgtttttg atttccttct
     3481 ttctatccaa gtaataacgc aaataaattt ctgcgtgttt tgcctgaaac atttgatgcc
     3541 aaatcttatc cctcattact ttcaaagtta caatttttgt aattttaggc acataattta
     3601 gttgaaaagt tatacacaca aaaaaattga agagatatga attctgacgg aaaagtatca
     3661 ttggaagaaa cactgaattt tataaatttt cttaccatga aaagtaagga aaagtctcct
     3721 gaatcataca ttgctataaa aaatgcaata aatattttca gcattccaac gcaacgattt
     3781 ttatttaaaa agagtaattt tttgtataga gcacacaaca atgataaaac ccctcggttt
     3841 ttttccgata cccgacttgg ggcaaataga cacccaaata caatcgacaa atttggcagg
     3901 gctaatgaac cgggtcagag cgtattctac tgctcagaca attgctatgt agcctatatg
     3961 gagacttctc acgtagttag gaatactgat aagccgctga agcataaaat gacaatcgga
     4021 acctgggaag ttgtggaaga tttgcgcttg gcgactctac caagtaactc gactatagct
     4081 ggattaaatc caactgtaga tgcattgcac aaaaattata tggaattggt tgaacaatat
     4141 agagatggca caacagattc aactttagtc ctgttagact atttctcaag aatgttcagt
     4201 agcgaggctg atggtgactc caccaactat ctcataagtt gtgcaattgc aaattactac
     4261 tatgagaatt tagtatttga ttatgaactt caaaaagcaa tatatttaga tggaattatt
     4321 taccctagtg ttatgctaaa aactgaaggc gtaaatttgg ctttaaccta cgatacatat
     4381 aattccaggg aaccagtaaa attgaaatta gttgatgttg ttgaattaac aatggtgcgc
     4441 gttggtgaca aaacctatga agaagaaact atagtaaagt gtaaatcaat aaatcaggaa
     4501 actggggaga tactctggta gctagataaa attaaaagtt atccacataa ttcacctaaa
     4561 gtagcaaatt taccctcctt acaactcacc attccacttg ctatttttaa attatggaaa
     4621 ataaaggtcg atatattcca aacaatctta tcaaatatcg caagatgctc ggactcaagc
     4681 aggaagaagt tgcttcgtat ctcgggttaa aaagtacaaa tagattatcc cgatgggaac
     4741 aaggtttgtc catgccaagt gcacaaaatc ttttcaagct aagcacacta tatcacactc
     4801 tcattgatca gctctatagc gatcatgtct cacacattaa gcgcaatatg tctgagaatg
     4861 aagaactgct tgattcttcg catccaaaaa aataaagaaa taataaccgt attgtaattg
     4921 tgtaattacg cattgtttta tgtgcttttt tcttgcactt aaattgcaaa aatagcacct
     4981 gtggaaaact cctgaaaaaa caatcgttcg taatcgaatc ggtttattta tataaaataa
     5041 ataaacaacg aacgagtaga agttttccac agttttagca gttaaccatt gttcattaac
     5101 aacatgatac atcacaaatt ggcgtatgaa attgcgtcag cacttggaga tactgacaac
     5161 ttaaaggtgc acgaggattt tactgaaacc tatcaagagg aatttcttcg taacacgctg
     5221 aataaggtaa tgtcaatacc tgaaagaaaa atcaaaaaaa cgcgcggggc attatttacc
     5281 tgcctggttc aacgaaatgg gaggggtaac cattctaggg attagtcccg gcacaagata
     5341 tatcggtata gcactacaac gagatggcat tctttatgat tggagaataa aatcatacaa
     5401 gggtgtgtgg tctgaagaaa aactgtcaaa agtggttacc tatatcgaaa accttatact
     5461 gacacatgtc gtagagcata tagcatgcaa gataccacat attggtaggt gtagtgaagg
     5521 gttaaaactt ctcatagaaa aaatcaaatc aattgcagag aagtgcaatt ccaaaataca
     5581 tctttactct atttcagaac taaaagaatt gtttacctcg agtattcaaa ataaaggagc
     5641 actagtaaac tatttagctg aaaaattgcc tgaattgagg catctcttga aaaaagagct
     5701 gagaaataaa aacacgtatt atgtaaaagt atttgaagct attgctacca ccgtgcattg
     5761 tcacaacagt ttgaaataaa cttatgagcc ccgcttaaaa gccaatcagt tttgattggc
     5821 ttttaataat ttttctatgt actttttaaa aatagaattc attaacttta cttagtcaaa
     5881 aattaaacag cttacattta catggaatca gaatattata taaccgcttt ttttggatta
     5941 attggtaccc ttattggtgc aggtatcagc ttttttgcca atgtatatca aagttctaaa
     6001 gaagcaaaat taagagtgcg tgaaaaaata tttgataaaa gaatcaatac tcatgaagag
     6061 attatagcct tttgtcaggt aatgagaaca acagtttcta ctcatgaggg agatgcacaa
     6121 aataatgtga tcacttaccc catgctactg ataaacagag agcattacgg taattttatt
     6181 aagaattttg ttcaagtgtt aggtagaaat agtatttgga tggacattga actttatcgg
     6241 tatatgaatt acattcagga ttatcttgaa agcctatcta gtaacataag ggacattgag
     6301 gaaaaaagtt ttcctttagt tggagtcttg attaaacagg attttatcaa tttggcttta
     6361 gagcttgaaa aagaagcatt taagttcttc gaaaaagaca tatataatat caacctaaag
     6421 atgaagaaag gacatcacaa atttccaaga gaggaaactg ttaaaaaatt taagaataca
     6481 cagttaaata aaaatatcgc cgcaataaag aatattcaaa taaaataaca accggaaatt
     6541 cataataata tgaacacaca atcaatatta gattttatcg aaagcttagg aattatagtt
     6601 gcttctgtca cagccacttt gggtttatat gcttggcgaa aagaagtgaa atggaaaaga
     6661 aggaatgaag tcgccgaaga aattctatat aatttatacg atgtaagaga taggttacgt
     6721 gctatacgtc atccctttgc gcatccttct gaaggagaaa ctcgactagt aacaaaacta
     6781 gagagtgtgg aagaaaaaac aatccgtaat tatgcatata ccacagtaga gcggtataac
     6841 aataaccgag aaccgttctt aaaccttgaa aaattaaaac atagatatgc aatatacttt
     6901 ggtcacaaaa atgcgtcttc aattacaaat attatatttt taaaaaatga aattatttct
     6961 gcttcataca aatatgctca acgttcaatt gaacgagata aattgattaa aaaaaatctt
     7021 ggtgaaggtt atattcagaa tcaaatcaat gctcttgata cacaaattga aaaatacgcc
     7081 tcaattattt gggagaaata tgaagacgat gatgctatat cttctgcaat tgatgagaca
     7141 atcgcaaacc aggaaatagt tttgaataaa tatgttaaat aacaaaggtc aggaatttac
     7201 tgtttttagt tttttctacc ttgcataata cacaaagtca gttatcccca aaatttgcta
     7261 taaatagcaa atttaccccc cttacccatt cctccacaaa atcatagact aatttggaa
//
LOCUS       DAORKW010000131         7298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_319_length_7298_cov_5.378020, whole genome shotgun sequence.
ACCESSION   DAORKW010000131 DAORKW010000000
VERSION     DAORKW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_319_length_7298_cov_5.378020"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..972
                     /gene="cysD"
                     /locus_tag="PLB46_06810"
     CDS             67..972
                     /gene="cysD"
                     /locus_tag="PLB46_06810"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRF76263.1"
                     /translation="MSYSLTHLRELEAESIYVMREVAAQFQKPALLFSGGKDSIVMVY
                     LARKAFWPAKFPFPLVHIDTGHNFEETMVYRDWLVKEMGANLIVGSVEESINKGTAVE
                     EKGLNASRNALQTVTLLETLEKEKFDAAMGGARRDEEKARAKERFFSHRDEFGQWDPK
                     NQRPELWNLFNGKKHIGEHFRVFPISNWTEMDVWQYIYQENIPLPKLYFSHQREVIVR
                     DGALISTHPEWVKVRPNETPKTMTVRFRTCGDLPITGAVESDADTVEKIIYEVATTRV
                     TERGSRADDKRSETSMEDRKKAGYF"
     gene            1034..2332
                     /gene="cysN"
                     /locus_tag="PLB46_06815"
     CDS             1034..2332
                     /gene="cysN"
                     /locus_tag="PLB46_06815"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HRF76264.1"
                     /translation="MEHLVQDGKFDHHGYLNMELLRFTTAGSVDDGKSTLIGRLLYDS
                     KSIFQDQLEAIEKSSKSRGEDYVNLALLTDGLRSEREQGITIDVAYRYFSTPKRKFII
                     ADTPGHIQYTRNMVTGASTANLAIILVDARHGVVEQTYRHSFIASLLQIPHIVICINK
                     MDLVDYSKEKYDEVVEQFRHFASKLDVQDVRFIPISALLGDNVVDRSTKMPWYEGPTL
                     MYTLETVHIGSDHNLIDTRFPVQHVIRPMSNEYHDYRGYAGRVAGGVLKPGDEVMHLP
                     SGITTKIAAIKTIDGDLAEAYPPQSVTITLEDDVDISRGDMIVRENNVPKIGQDIDIM
                     VCWMDDKNPLRLNGKYSIRHTTRDARCIIKEILYKVDVNTLHRIQDVDSLSLNEIGRV
                     KIRTTVPLFYDEYRRNRNTGSLIIIDEGSNTTVGAGMIIE"
     gene            2466..2834
                     /locus_tag="PLB46_06820"
     CDS             2466..2834
                     /locus_tag="PLB46_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRF76265.1"
                     /translation="MKELTKAEEQVMQILWDLEKAFVKDVLEQLPEPKPAYTTVSTII
                     RILEQKEFVGYKAYGKTHEYFPLVSKDAYKKFITKGLLKDYFSGSLKNLVSHFAEKEK
                     IDVKELDEILKIIEQTKKKK"
     gene            2839..4011
                     /locus_tag="PLB46_06825"
     CDS             2839..4011
                     /locus_tag="PLB46_06825"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRF76266.1"
                     /translation="MLYLIKLNITLIACFLLYKGVFQHGTTLQARRFFLLFSLVFAII
                     IPFAATIHLRDNIAAVTTVLSTVNIQSNALAENVSVTGSYSQYITGAYIGIVIIMFLS
                     AVYKVWSLFRIIRQSSVEKTRQYKLISNSAISAPASFFNYIFMPENLDAKSYSLILIH
                     ERLHVKQWHSLDVLFTEILKWVCWFNPVAYLFQKEIRFVHECIADKAAGETQQVTYQQ
                     LLVQFHLSASVNTLTNHFNQTTNLKRRITMFNQQFSIANNAIKLALVLPFFVCIGILQ
                     SGAQTSEKSKTTTEEGVTNPEFPGGINELMSYLASNIVYPEAAKAANIQGTSIIEFTV
                     AKNGKVKDVHVKKSANPELDAEAVRVVSTMPNWKPGEKEGKKTDVVQILPIRFALN"
     gene            complement(4067..7141)
                     /locus_tag="PLB46_06830"
     CDS             complement(4067..7141)
                     /locus_tag="PLB46_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF76267.1"
                     /translation="MKRVFTALCFLSIPILSQAQPVVIGNTTYDFQTYSGNHNRIIAY
                     TDGKITAGWLGSDGFAAAFNDRGMFVNHFNGASWGAFPANRAESKKSYDGDLLRVLDH
                     EVIVTDETSTVMVHKNSSVGATDWTATAGSEDIDGYYPVSYCPAGTDDIYVVAAKKTT
                     QESLMFNRSDDGGETWAVAEYALPFAEEAYGIEFIASACYQVVAKGNDVYVLYGANFC
                     DMILMHSDSKGDIGTWTSQVLVNFPIDNFDGAAGETTDYDGDGDKDTIASSDGKLEMI
                     IEDDGTVHVFSGRIRIYDPTTAAGYFYIPKTDGIWYWKTGMASAVVLDAFVDWNNADG
                     LNNPYGGIGTDFTAYGAEGVTSFPTAAWDPITDKVFLMYMSPVEYQISLGNQTRYDVF
                     GMYTEDDGDTWTNPVNLTYTTHLTKENAYPSAYPRVVDGAVHVTWQEDAEPGTALDVP
                     ADAIITSNMLYAAWPPERFDPYNPTVDFTYVLVPFGGSFNATFTNLTVDAETYYWTFG
                     DGGTSTLKNPTHTYVPGEYEVCLTAYNVYGEATVCETIVAFNEPVADFTSIGDPEVAF
                     IDLSSGDPTSYFWDFDDGFTSTESDPVHTFVVNGTYNVCLTVSNVAGSDTHCEDIIID
                     SYLSPTAYFIYSGDPTVTFTDLSIGGPTSWAWNFDDGFTSTLQNPTHTYAANGVYNVC
                     LTATNGVGSNTTCQDVTIDYYVVPVVDFSFTGDPTTTFTDLSTNSPIEWAWDFDDGFT
                     STLQNPVHTFPTNGSYDVCLTATNLTGSATECKVVIIDGYLAPVATFNYTGDPIVTFN
                     DLSTNSPTSWFWDFGDGDFSSLENPVHDFVENGTYNVCLTVSNPGGVDEDCQNVVIDN
                     GASAPVVDFEVDTIIGLTVSFSDLSINEPTDWLWDFGDGAISGLQNPVHTYAVLDIYT
                     VCLTATNDIGSEQLCKQIDIATGINQTPNLAINIYPNPASNIIVVDLPAYDEQMQISL
                     LNMAGQEIYITEKNMFQSSTNIQINVADLPSANYIIRLTIHDKIYLGKLFKD"
BASE COUNT         2376 a         1355 c         1374 g         2193 t
ORIGIN      
        1 caggaaagcg tgcaacgtgt aatcgatgtt agcataccta tcatacaaca ttcttaatta
       61 attgtcatga gttatagttt aacacattta agagaacttg aagctgagag tatctacgtg
      121 atgcgagaag tagctgcgca gttccaaaaa cctgccctgc tgttttcagg tggaaaagac
      181 tctatcgtta tggtttacct cgcaagaaaa gccttttggc ctgcgaaatt cccattccct
      241 ttagtgcata tcgacaccgg acacaatttt gaagaaacca tggtttaccg cgactggctg
      301 gtaaaagaaa tgggtgctaa cttaattgtg ggtagtgttg aagaatctat taataaaggt
      361 acagcagttg aagaaaaagg tttaaatgca agtcgtaatg ctttgcaaac cgtaacctta
      421 ctcgaaacac tcgaaaaaga aaaattcgac gctgctatgg gtggtgcccg acgcgatgaa
      481 gaaaaagcac gtgcgaaaga acgtttcttt tcacatcgtg atgaattcgg tcagtgggac
      541 ccaaaaaatc agcgccctga attatggaat ttatttaacg gtaaaaaaca tatcggcgaa
      601 catttccgtg tattcccaat ttcgaattgg actgagatgg atgtttggca atatatttat
      661 caggaaaata ttccacttcc gaaattatat ttctcacatc agcgtgaagt tattgtgcgc
      721 gatggtgcat taatttctac tcaccctgaa tgggttaaag ttcgtccaaa cgaaacacct
      781 aaaacaatga cggtgcgttt cagaacttgt ggcgatttac ctattaccgg tgcagttgaa
      841 agtgatgctg atacagtaga aaaaattatc tacgaggtgg caaccacacg tgtaactgaa
      901 cgcggaagtc gtgctgatga taaacgcagc gaaacttcaa tggaagacag aaaaaaagca
      961 ggttatttct aaatacaatt ttgaaattat cagcttaaat aaacaattta ttcaaacact
     1021 tatccaatta taaatggaac atttagttca agacggaaaa ttcgatcacc atggttattt
     1081 aaacatggaa ttgttgcgct ttaccactgc aggaagtgta gatgatggta aatcgacatt
     1141 aattggtcgc ttattatacg attcaaaatc aatttttcaa gatcaacttg aagcaattga
     1201 aaaatcaagt aaatctcgcg gtgaagatta tgtgaacctc gcattattaa ccgatggttt
     1261 acgcagcgaa cgcgaacaag gtattactat tgatgtggct tatcgttatt tcagcacacc
     1321 taaacgcaag tttattattg ctgatactcc cggccatatt caatataccc gtaacatggt
     1381 taccggtgct tctacagcca accttgctat tattttagta gatgctcgtc acggtgtagt
     1441 tgaacaaact tatcgtcact cattcatcgc ttcattatta caaattccgc atattgttat
     1501 ttgtattaat aaaatggacc ttgttgacta cagcaaagaa aaatacgatg aagtagttga
     1561 acagttccgt cactttgcat cgaaattaga tgtgcaggat gttcgtttta ttcctatcag
     1621 cgcattactt ggcgacaacg ttgttgaccg cagtacaaaa atgccttggt atgaaggacc
     1681 aacattaatg tatacactcg aaactgtaca cattggttct gaccataatt taattgatac
     1741 acgtttccct gtgcagcacg taattcgccc tatgagcaat gaatatcacg attaccgtgg
     1801 ttatgcaggt cgcgttgcag gtggtgtttt aaaaccgggt gatgaagtaa tgcatttacc
     1861 aagtggtatt actactaaaa ttgcagccat taaaacaatt gatggtgatt tagctgaagc
     1921 atatcctcca caatcagtta ccatcacttt agaagatgat gtagatatta gtcgaggtga
     1981 tatgattgtt cgtgaaaaca atgtgcctaa aatcggtcag gatatcgata tcatggtttg
     2041 ttggatggat gataaaaacc cattgcgttt aaatggtaaa tacagcattc gccatactac
     2101 tcgtgatgcc cgttgtatta ttaaagaaat actttacaaa gtagatgtta atacattaca
     2161 ccgtattcag gatgtagatt ctttatcgtt aaacgaaatt ggccgcgtaa aaatcagaac
     2221 aaccgttcca ctgttttacg atgaatatcg ccgtaaccgt aacactggtt ctcttattat
     2281 tatcgatgag ggaagcaata caactgtagg tgcgggaatg atcatagaat aacatttttg
     2341 accaaatcaa tatgtaaccc ggtagtgagg agctgccggg cttttttatg cccggttttc
     2401 tcagatttct gtcattttct aaaaaattag ttgtataact aaataattag ttataggttt
     2461 gtaacatgaa agaacttacc aaagcagaag agcaggtaat gcaaatatta tgggacctgg
     2521 aaaaggcctt tgtgaaagat gtattggaac agttacctga acctaaaccg gcttatacca
     2581 ctgtttccac catcattcgt attctggagc aaaaagaatt tgttggctat aaggcttatg
     2641 gtaaaacgca tgagtatttt cctttagtgt cgaaagatgc ttataaaaaa tttatcacga
     2701 aaggcttgtt aaaagattat ttcagcgggt cattaaaaaa cctcgtatcc cattttgctg
     2761 aaaaagaaaa aattgatgtg aaagaattgg atgagatatt aaaaattatt gaacaaacca
     2821 aaaagaaaaa ataagcttat gttatactta ataaaattga atattacgct tatagcctgc
     2881 tttcttttat ataaaggcgt atttcaacat ggtactactt tgcaggcacg ccgatttttt
     2941 ttgttgttta gtttagtgtt tgcaatcata attccttttg cagcaacaat tcatttacgt
     3001 gataatattg cagcagtaac aacagttttg tctacggtga atattcaaag taatgcactt
     3061 gcagaaaatg tttcggtaac aggcagttat tcacagtata ttacaggtgc ttatataggc
     3121 atagtaatta ttatgttttt atcagctgta tataaagtct ggtcgttgtt tcgaattatt
     3181 cgccaatcat ctgtcgaaaa aacgaggcaa tataaattaa tcagtaatag tgcgattagc
     3241 gcaccggcat cattctttaa ttatattttc atgcccgaaa atctggatgc caaatcgtat
     3301 tcactcattt taattcacga acgtttgcat gtaaaacaat ggcacagttt ggatgtgctt
     3361 tttacagaaa tactcaagtg ggtttgttgg tttaatccgg tggcgtattt gtttcaaaaa
     3421 gagatacgat ttgtacacga gtgtatagcc gacaaagcag cgggtgaaac acaacaagtt
     3481 acctatcagc aattattggt gcaatttcat ttatctgcat ctgtaaatac attaactaat
     3541 cattttaatc aaacaactaa tttaaaaagg aggatcacaa tgtttaatca acaattttca
     3601 attgccaaca atgctattaa attagcttta gtgttaccat tttttgtttg tattggtatt
     3661 ttgcaatcag gcgcacaaac ttcagaaaaa agtaaaacta caactgaaga aggtgttaca
     3721 aatcctgaat ttccgggcgg tatcaatgaa ttaatgagtt acctggcgag taatattgtt
     3781 tatcctgagg ctgcaaaagc ggcgaatatt cagggcacca gtattattga atttacggta
     3841 gctaaaaacg gtaaagtaaa agatgttcat gtgaaaaaaa gtgccaatcc ggaattagat
     3901 gcagaagctg ttcgcgtagt ttcgaccatg ccaaattgga aacccggtga aaaggaaggt
     3961 aaaaaaacag atgttgtgca aatattacca atacgtttcg cacttaatta aagtcaattt
     4021 tatatcatta aaaaaccccg caattgcggg gttttatttt gcttgattaa tccttaaaga
     4081 gtttccctaa ataaatttta tcgtgaattg ttaaccgaat aatataatta gctgacggca
     4141 aatctgctac atttatttga atatttgtac tggattggaa catatttttt tctgtaatat
     4201 aaatctcctg accagccata tttagtaacg aaatctgcat ttgttcatca taagcgggta
     4261 agtccacaac tatgatatta ctcgcaggat tcggataaat atttatagcc aaattgggtg
     4321 tctgattaat accggtagca atatcaattt gtttacaaag ttgttcgctt ccgatatcat
     4381 ttgtagcagt taaacaaacg gtataaatat ctaaaacggc gtaagtatgc acaggatttt
     4441 gtaatcctga aattgcacca tctccaaaat cccagagcca atcagttggt tcattaatgg
     4501 ataaatcgct aaatgaaacg gtgagtccga taatagtatc aacttcaaaa tctacaactg
     4561 gtgccgatgc gccattatcg ataacaacat tctgacaatc ttcatctact ccacccggat
     4621 ttgacacagt taaacaaacg ttataagtgc cattttcaac aaaatcgtgt accggatttt
     4681 cgagtgaact aaaatcgcca tcaccaaaat cccagaacca tgatgttggt gagttggtac
     4741 ttaaatcgtt aaatgtaaca atcgggtcgc ctgtataatt aaatgttgcc accggagcaa
     4801 gataaccatc aataattacc actttacatt cagttgcaga acctgttaag ttggttgcgg
     4861 ttaaacaaac atcatagcta ccatttgtag gaaatgtgtg caccggattt tgtaatgtag
     4921 aagtaaatcc atcatcaaaa tcccaagccc attcaattgg tgaatttgta cttaaatcgg
     4981 tgaacgttgt agttgggtca ccggtaaatg aaaaatctac aacaggcaca acataataat
     5041 ctattgttac atcctgacaa gtggtatttg agccaacgcc atttgttgcg gttaaacaaa
     5101 cattatatac gccgttggct gcataagtat gtgttggatt ttgtaaagta gaagtaaatc
     5161 catcatcaaa attccaagcc catgaagttg gtccgccaat tgataaatcg gtgaaagtta
     5221 ctgttgggtc gccactataa ataaaataag ctgttggtga taaataacta tctataataa
     5281 tatcttcaca atgtgtatca gagccggcca catttgaaac agttaaacaa acattataag
     5341 taccgtttac aacaaatgta tgaacggggt ctgattcagt tgatgtaaat ccatcatcaa
     5401 aatcccaaaa ataactagtt gggtcaccac tacttaaatc gataaatgca acctcagggt
     5461 caccaattga agtaaagtcc gcaaccggtt cattaaatgc aacaattgtt tcacaaactg
     5521 ttgcttcacc atatacatta taggcagtta aacatacttc atattcaccg ggaacataag
     5581 tatgggttgg attttttaat gttgatgttc cgccatcgcc aaaagtccaa taataggttt
     5641 cagcatcaac agttaagttg gtgaatgtcg cattaaaaga accaccgaaa ggaactaata
     5701 cataagtaaa atcaactgtt ggattatatg gatcgaaacg ttcaggtggc catgctgcat
     5761 ataacatatt acttgtaata atggcatcgg caggaacgtc gagtgctgtt cccggttctg
     5821 catcttcttg ccaggtaaca tgcacagccc catctacaac tcgcggataa gcgcttggat
     5881 atgcattttc tttggtaaga tgtgtggtat aagttaagtt aacaggattt gtccaagtgt
     5941 ctccatcatc ttcagtatac attccaaaaa catcgtaacg cgtttgattg cctagtgaaa
     6001 tttgatattc cacagggctc atatacatta aaaatacttt atctgtaatt gggtcccatg
     6061 ctgctgtggg aaatgaagta acaccttctg ctccatatgc tgtaaaatct gttccaattc
     6121 caccataagg attatttaag ccatcggcat tattccaatc tacaaaagca tctaacacga
     6181 ctgcagatgc cataccggtt ttccagtacc atataccatc tgtttttggg atataaaaat
     6241 aaccggcggc agtagttggg tcatatatgc gaatgcgacc gctgaataca tgaacggtgc
     6301 catcatcttc aataatcatt tctaattttc catcgcttga agcaatggta tctttatctc
     6361 catcgccatc gtaatcggta gtttcgccag cagcgccatc aaaattatcg atagggaagt
     6421 ttactaatac ctgacttgtc caggtgccaa tatctccttt tgaatcagaa tgcattaaaa
     6481 tcatatcaca aaaattggcg ccgtataaaa catatacgtc attacctttt gcaacaacct
     6541 gataacaggc agatgcaatg aattcaatac cataagcttc ttcagcaaat ggtaatgcat
     6601 attctgcaac tgcccatgtt tcaccaccgt cgtcggagcg attaaacatg agtgattcct
     6661 gcgtagtttt tttagcggcg actacataaa tgtcatccgt acctgcaggg caataagaaa
     6721 ccggatagta accatcaata tcttctgaac cggctgttgc tgtccagtct gtggcaccaa
     6781 cactgctatt tttatgtacc ataacagtcg atgtttcatc ggttacaata acttcatggt
     6841 cgagcacccg caacaaatcg ccatcgtacg attttttgct ttctgcccga ttagcaggaa
     6901 atgcacccca agaggcgcca ttaaaatgat taacaaacat accgcggtcg ttaaacgcgg
     6961 cagcaaaacc atcagaacct aaccatccgg cagtaatttt tccatcggtg taagcaataa
     7021 tccggttatg attaccgctg taggtttgaa agtcgtatgt ggtgttcccg ataacaacgg
     7081 gttgtgcctg ggagagtatg ggaatggata aaaaacataa ggctgtaaat actcttttca
     7141 tatgatcaca tttattaaaa caatgaacta taggtaatag gcattataat attagctaat
     7201 aaccccatta agttcaaagc gcaaggtatt tacctagatt aatatgatgt cacaaattcc
     7261 cataaaataa aaaagcgggc aattgcccgc ttttttat
//
LOCUS       DAORKW010000132         7241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_324_length_7241_cov_5.668244, whole genome shotgun sequence.
ACCESSION   DAORKW010000132 DAORKW010000000
VERSION     DAORKW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_324_length_7241_cov_5.668244"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="PLB46_06835"
     CDS             <1..1329
                     /locus_tag="PLB46_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76268.1"
                     /translation="GTSWDNITSGTLNGLHPAAIVHQFGTDGGVYLTVREAAVFYRTN
                     TFLDWIQHGDDLPLVTDALKTIPFYRDNKLRLASWNIGIWEAPLFEPSALIADFSTAS
                     PYFYCGGDEVKFVDRSTASATATYAWEFPGAIPSSSTEKNPVVTYPTGGSFNVTLTIN
                     DGASVASITKTAIVSSLPESILPLAENFETGTFASGWHGGESGGWAIADFASAYAIGT
                     SSMYFNNYYTDLQGGYVEEISAKYNMEDVITATLNFDVAYRPYGGIYMDTLQILASTD
                     CGATFTEIFYAGGNDLSTGPVYTADVFYPEADEWETYTIDMTSFAGADNLTLYFKNIG
                     YWGQALYLDNININANFIPITEFEQQVAAIIVPNPNQGYFNLATECLQTQQAEIKLTN
                     ALGEIIYTTQQQLNVGDNTTVFDVQHLSKGVYFAVIQTGKQLTTVPFVIE"
     gene            complement(1394..3439)
                     /locus_tag="PLB46_06840"
     CDS             complement(1394..3439)
                     /locus_tag="PLB46_06840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HRF76269.1"
                     /translation="MNYKALLFLCITVGIFSCKPKEKTADVNYDNFLNEYIDSTVSPG
                     EDFFKFAMGKWLKNNPIPESERSWGIWTLVNEENYARIRKINEEAAANTKAKKGSNEQ
                     KIGDFWFTGMDTVTIEKQGITPLKPELDKIAAISDPQGVIKMAAYLHTMQVQPLFGNY
                     IFQDEKNSSKFRLHFYQGGIGLPDRDYYFDKDERTLTIRDEYVKHISKMFQLTGIDAA
                     TADTYAQTIMKMETDFAGASRKLADLRDPYANYNSMAVADVNKLCPAINWESFLTDAG
                     IKNIDTIIVGQPEFFKQLNTSLTKESVDNWKIYLQWNLINAYADRLSSDFDKEHFHFF
                     GTILNGTKEQRARWKRVLDAEEDAMGFLLGQLYVEAYYPPEEKARYEKIVENTMAAYK
                     VRIQQLDWMSPETKEKALLKLSTVIKKVGYPDKWRDYSALTITRDSYVQNAINANKYL
                     HAYEVEKLYKPVDKTEWEMTPQTWNAYYNPSNNEIVLPAAAFIVPGVPDKYVDDAIAY
                     GYAAGSTIGHEITHGFDDQGSQFDEKGNLSEWWTPQDRAQFLERTKLIVEQFNNYIII
                     DTIHVNGEATQGENIADLGGMLIGLEAFKMTDQYKEGKVIGGYTPVQRYFLGFALSWY
                     GKYRDEAMVLRAKTDVHSPNFLRVNGPASNIQEFYDAFGVKPGDKMYVPDSLRIKIW"
     gene            3583..4707
                     /locus_tag="PLB46_06845"
     CDS             3583..4707
                     /locus_tag="PLB46_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRF76270.1"
                     /translation="MNALRRTYLLLFIIGLGFFIAACTTKAENDNTISKQTDIPENAP
                     DTITPITPSDGPPGMVWIPGGEFIMGTDTDPQHRTDESPAHKVKVDGFWIGITEVTNA
                     EFAKFVAATGYKTTSERPIDWEELKQQVPPGTPKPADELLQPASMVFTTTTAPVDLNN
                     YLNWWSFIPGADWRHPQGPNTSIIGKEQHPVVQVSWDDAVAYCKWAGKRLPTEAEWEY
                     AARGGVAGAIYPWGNDKDLSKHTNSWQGNFPNLNTAEDGFKTTAPVKSYAPNKYGLYD
                     MAGNVWEWCSDFYHVDYYKTCAKQEVSLNPQGPAKPYDPNQPYNIVRSKRGGSFLCNE
                     VYCSSYRVAARMATSYDTGQDHSGFRCVMTQEMWEKQKNK"
     gene            4810..7191
                     /locus_tag="PLB46_06850"
     CDS             4810..7191
                     /locus_tag="PLB46_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF76271.1"
                     /translation="MRSFLLMLLLCFAAIGQAQTGKIYGTVTNSINNEPLPFVTVLLT
                     GTSFGAVTDIDGNYTIDNLAPGLYNVEFSFIGFKKKTVFEVQVTNAKSVYLNASLDED
                     NNVLEAVEVKAVITDKTEESPLSLRTIGANEIQRNPGGNRDISRVIQSLPGVTYTPSF
                     RNDIIIRGGAPNENRFYLDGIEVPNINHFATQGSSGGPVGMINVDFIREVEFYSGAFP
                     VNYGNALSSVFSFEQRDGRTDKWGGNFTVGSSDIGLTAEGPLGEKSSVLLSARRSYLQ
                     FLFDAIGLPFLPTYNDFQYKQKIKINQKNELTIIGLGAIDEFGLNLQDDTSAFQQYIL
                     GNLPFQTQWNYTIGASYKHYRERGYSTIVASRNMLNNRAYKYIDNDDSNESNLIFDYT
                     SREMENKFRYEETLDIKRFRIKGGVNYELARYTNNTYQLIPVGADVITVDYQSELPLQ
                     KWGAFVQSSTSIFDYRLTLSFGLRADANNYASSMQNLLDQLSPRFSASYKLTDTWSAN
                     FNTGIYYQLPAYTVLGYAEDENNFINKANNITYIQSNHLVGGLEYRPSNYLRFTLEGF
                     YKTYNNYPFSIRDSVSLANLGADFGVIGDEPVSSTSNGRSYGVEFLAQQKLNKNFYGI
                     LSLTYVVSEFEDKNGNYAASSWDNRTIISLTAGKKFKHDWEAGLKWRFSGGLPYTPYD
                     VEYSSLIYVWDINRQGVLDFDQLNQKRLDPYHQLDIRVDKKWFFNKWNLDVYVDIQNV
                     YNFQTTLQPFVDVVRDDIGNPVISATDPTRYEVYQLENTSGTLLPSIGLIIEL"
BASE COUNT         2258 a         1351 c         1291 g         2341 t
ORIGIN      
        1 ggaacatctt gggataatat taccagcggc acactaaatg gattacatcc tgcagcaatc
       61 gttcatcaat ttggaaccga tggcggtgtt tatttaactg tcagagaagc ggcagtattt
      121 tatcgcacca atacattttt agactggatt caacatgggg atgatttacc tttggttact
      181 gatgcattaa aaacaattcc attttatcgc gataataaat tgcgtctggc aagttggaat
      241 attggtattt gggaagcacc attatttgaa ccttctgcat taattgccga tttttctaca
      301 gcttcacctt atttttattg tggtggtgat gaagtaaaat ttgtcgaccg ctcaacagca
      361 tcggcaaccg caacttatgc ttgggaattt ccgggtgcaa taccatcatc ttctaccgaa
      421 aaaaatcctg ttgttaccta tccaacaggt ggtagtttta atgttacatt aactattaat
      481 gatggagcaa gtgttgcctc aattaccaaa acagcaattg ttagttcatt accggaatca
      541 attcttccat tagctgaaaa ttttgaaaca ggaacttttg catcaggctg gcatggtggc
      601 gaatcaggcg gttgggcaat tgcagatttt gcaagtgctt atgctattgg tacttccagc
      661 atgtatttca ataattatta taccgactta caaggtggat atgttgaaga aatatctgca
      721 aaatataata tggaagatgt tatcactgca acattaaatt ttgatgtagc gtatcgccct
      781 tacggtggta tatatatgga tacattacaa atattagctt caaccgattg tggtgcaact
      841 ttcaccgaaa tattttatgc aggtggcaac gatttatcta ccggccctgt ttacactgca
      901 gatgtttttt acccggaagc agatgaatgg gaaacatata ccattgatat gacctcattt
      961 gcaggtgcag ataatttaac cttatatttt aaaaatattg gttattgggg acaagcatta
     1021 taccttgata acattaatat aaatgccaat tttattccta ttactgaatt cgaacaacag
     1081 gtagctgcta ttattgttcc aaatcctaat caaggatatt ttaatttggc aactgaatgt
     1141 ttacaaacgc agcaggcaga aattaaactc acaaatgcac ttggagagat tatttataca
     1201 acgcaacaac aactaaatgt cggcgataat acaaccgtat tcgatgttca acatttaagt
     1261 aaaggtgtgt atttcgcagt tattcaaaca ggcaaacaat taaccacagt accatttgta
     1321 attgaataaa caatttaacc ataaaaaaag cccgacactt aaatgtatcg ggcttttttt
     1381 ataccggttt tcattaccat atttttattc ttaaactatc cggaacatac atcttatcgc
     1441 cgggtttaac accaaaggca tcataaaatt cctgaatatt actggcaggt ccgtttaccc
     1501 ttaagaaatt aggtgaatgc acatccgttt ttgcacgtaa caccattgct tcatcacgat
     1561 attttccata ccatgataat gcaaatccga gaaaatatct ttgcacaggt gtatatccac
     1621 caattacttt cccttcttta tattggtcgg tcattttaaa agcttccaat cctatcagca
     1681 ttccgccaag gtcagcaata ttttcacctt gtgttgcttc accatttacg tgaatagtat
     1741 cgataataat ataattatta aattgttcaa caatcaattt tgtcctttcc aaaaattgtg
     1801 ctctatcctg aggtgtccac cattctgata aattaccttt ttcatcgaac tgacttccct
     1861 ggtcatcaaa accgtgcgta atttcatgtc caatagtaga accggctgca taaccataag
     1921 caatagcatc atccacatat ttatcaggta ctcccggaac aataaatgcc gcagccggta
     1981 atactatttc attattactt ggattataat aagcattcca ggtttgtggg gtcatttccc
     2041 attcagtttt atctacaggc ttgtataatt tttctacttc ataagcatgt aaatatttat
     2101 tcgcattaat agcattttgc acatacgaat cacgtgttat agttaatgca gaataatctc
     2161 tccacttatc cggatatcct acttttttaa ttacggtgct taatttcaat aacgcttttt
     2221 cttttgtttc agggctcatc caatctaact gttggatgcg aaccttataa gcagccatgg
     2281 tattttctac aattttttca taacgcgctt tttcttccgg agggtaatat gcctcaacat
     2341 ataattggcc gagtaaaaat cccattgcat cttcctctgc atctaataca cgtttccatc
     2401 tggctctttg ttctttggtg ccatttaata tggtgccgaa aaaatgaaaa tgttctttat
     2461 caaaatcact gctcaaacgg tcggcatatg cattaattaa attccattgt aaataaattt
     2521 tccaattgtc caccgactct tttgttaagg atgtatttaa ttgtttgaaa aattccggct
     2581 gaccaacaat aatggtatct atatttttta ttcctgcatc agttaaaaat gattcccaat
     2641 taatagcagg acataattta tttacatctg caacagccat actgttataa ttagcataag
     2701 ggtcacgtaa atcagctaat tttctggatg cgccggcgaa gtcagtttcc attttcataa
     2761 tggtttgcgc ataagtatct gccgttgctg catcaatacc ggttaattga aacatttttg
     2821 aaatatgttt tacatattca tcgcgtatag ttaaagtgcg ttcatcttta tcaaaataat
     2881 aatctctgtc aggtaaacct ataccgccct gataaaaatg caatcgaaat ttgctgctgt
     2941 ttttttcatc ctgaaaaata taattgccaa acaaaggttg cacctgcata gtatgtaaat
     3001 aagctgccat tttaattacc ccttgagggt cactgattgc agctatttta tctaattcag
     3061 gttttaatgg tgttatgcct tgtttttcaa tggtaacagt atccattccc gtaaaccaaa
     3121 agtcgccgat tttttgctca ttactccctt tttttgcttt tgtgttagca gcagcttctt
     3181 catttatttt acgaatacgt gcataattct cttcattcac caaggtccaa attccccaag
     3241 agcgttcgct ttcaggaatg ggattatttt ttaaccattt tcccatggca aatttgaaaa
     3301 aatcttctcc tgggctaaca gtcgaatcga tatattcatt taaaaaatta tcgtaattaa
     3361 catcagcggt tttttccttt ggcttgcagg aaaaaatacc gacagtaata cataaaaaca
     3421 gcagtgcttt gtaattcata ttgtaatgtt taagtttcaa atataggtat atccttcaat
     3481 acagttactt aacagttgtt aaagcagcca aatttccgga atgataaaaa tcaattgtgt
     3541 gaacaggtta ctgccataaa aattgtaatt atctttgtat aaatgaatgc actaaggcgc
     3601 acatatttac tcctgtttat aatcggtttg ggatttttca ttgctgcttg cacaaccaaa
     3661 gccgaaaatg ataataccat ttctaaacaa acagatattc ctgaaaatgc gcctgatact
     3721 atcacaccta taactccttc tgacggccct cccggtatgg tttggatacc cggtggtgaa
     3781 tttataatgg gcaccgatac cgaccctcag catagaactg atgaaagccc tgcgcataaa
     3841 gttaaagtgg atggtttttg gataggcatt accgaagtaa ccaatgcgga atttgccaaa
     3901 tttgtcgcag caaccggtta taaaaccacc tccgaacgtc ctattgattg ggaagaacta
     3961 aaacaacagg ttcctcccgg cactcctaaa cctgctgatg aattattaca gcctgccagt
     4021 atggtattta ccaccactac tgcgcctgtc gacctgaata attatttaaa ctggtggagt
     4081 tttattccag gggctgattg gcgacaccct caaggaccaa atacatcaat tataggtaaa
     4141 gaacagcatc ctgttgttca ggttagttgg gacgatgcag ttgcgtattg taaatgggca
     4201 gggaaacgat tgccaactga agcagagtgg gaatatgcag ctcgcggtgg agttgctgga
     4261 gctatttatc cttggggaaa cgataaagat ttatctaaac atactaactc atggcaaggt
     4321 aatttcccta atctcaatac cgcagaagat ggtttcaaaa ctactgcacc ggttaaatct
     4381 tatgcgccaa ataaatatgg tttatatgat atggccggaa atgtctggga atggtgcagc
     4441 gatttttatc atgtagacta ttataaaacg tgtgcaaaac aagaagttag tttaaacccg
     4501 caaggaccgg caaaacctta cgaccctaat caaccttata atattgttcg gtcgaagcgt
     4561 ggtggctcat ttttatgtaa tgaagtttac tgttcatcat atcgtgttgc agctcgcatg
     4621 gcgactagtt atgataccgg tcaggatcac tccggtttcc gttgtgtgat gactcaggag
     4681 atgtgggaaa aacaaaaaaa taaatgatct tcctattttt agtttgatgc cataatccac
     4741 taggcaataa agtaaaaaaa tgtagatata ttctttaaac aaattattca aaaccatgtt
     4801 tatccattca tgagaagttt tttattgatg ctgcttcttt gttttgcagc cataggtcaa
     4861 gcccaaacag gaaaaattta cgggacagtc accaattcca ttaataatga acccctgcca
     4921 tttgtcactg tattattaac cggaacaagc tttggagccg taactgatat agatggaaat
     4981 tacactatcg ataatctagc tccgggactt tacaatgtag aattcagctt tattggattt
     5041 aagaaaaaaa ctgtttttga agtgcaggta accaatgcaa aatcggtgta tttaaatgcc
     5101 tctcttgatg aagataataa tgttctcgaa gctgttgaag taaaagcggt aattacagat
     5161 aaaacagaag aatcgccatt atcacttcgt accatcggtg cgaatgaaat tcaacgtaat
     5221 ccaggcggta atcgcgatat tagtcgggtt attcaaagtt taccgggtgt aacgtataca
     5281 ccatcttttc gcaacgatat tattattcgc ggaggtgcac ctaatgaaaa tagattttat
     5341 ttagatggca ttgaagtgcc aaatattaat cactttgcta cccaaggttc cagtggcggt
     5401 ccggttggaa tgattaatgt agattttatt cgtgaagtag aattttattc cggtgcattc
     5461 ccggttaatt atggcaatgc acttagcagt gtattttcat ttgaacaacg tgatggcaga
     5521 acagacaaat ggggaggtaa ttttactgta ggttcaagtg atattggttt aaccgcagaa
     5581 ggtccgcttg gcgaaaaatc ttcagtatta ctatccgcac gaagaagtta tttacaattt
     5641 ttatttgatg cgattggttt accatttttg ccgacataca atgattttca atataagcaa
     5701 aaaattaaaa tcaatcaaaa aaatgaactc accatcattg gcttaggtgc tatagatgag
     5761 tttggactta atttacaaga cgataccagt gcgtttcaac aatatatatt aggtaattta
     5821 ccttttcaaa cacagtggaa ctatactatt ggcgccagct acaaacatta tcgcgaacgt
     5881 ggatattcaa ccattgttgc cagtagaaat atgctgaaca atcgcgctta taaatatatt
     5941 gacaacgatg atagcaacga atcaaatctt attttcgatt ataccagccg cgaaatggaa
     6001 aataaatttc gttacgaaga aactttagac attaaacgat tcagaattaa gggcggagtt
     6061 aattatgaac ttgcgcgcta caccaataat acttaccaat taattccggt aggtgcagat
     6121 gtaattaccg tagattatca atctgaactt ccattacaaa aatggggagc atttgtgcaa
     6181 tccagcactt cgatttttga ttatcgctta accttgtcgt ttggcttacg tgccgatgcc
     6241 aataattatg cttcgagtat gcaaaatcta ctcgaccagt tatctccgcg tttttctgca
     6301 agttacaaat tgaccgatac atggagtgcc aattttaata ccggcatata ttatcagctt
     6361 cctgcttata ccgttttagg atacgctgaa gatgaaaata attttatcaa taaagcgaat
     6421 aatattactt acatccaatc caatcatttg gtaggtggat tagaataccg tcctagtaat
     6481 tatttgcgat ttactttaga aggtttttac aaaacttaca acaattatcc attcagcatt
     6541 cgcgattctg tttcgttggc caatttaggt gctgattttg gtgtaattgg cgatgaacct
     6601 gtttcatcaa ctagcaacgg aagaagttat ggagtggaat ttttagctca acaaaaatta
     6661 aacaaaaact tttatggtat tttatcattg acctatgtag tcagcgaatt tgaagataaa
     6721 aatggcaatt atgccgcttc ttcctgggat aacagaacaa tcatttcgtt aactgccggt
     6781 aaaaaattca aacacgattg ggaggcaggt ttaaaatggc gttttagcgg tggtttaccg
     6841 tatacacctt acgatgtaga atacagttca ttaatttatg tttgggatat taatcgccaa
     6901 ggggttttgg attttgacca gttaaatcaa aaacgtttag acccttacca tcaattagat
     6961 attcgggtag ataaaaaatg gttttttaat aaatggaatt tagatgttta cgtggatatt
     7021 caaaatgtgt acaatttcca aactacttta caaccatttg tagatgttgt gagagatgat
     7081 atcggcaatc cggttatcag tgccacagac ccaactcgtt acgaagtata ccaactggaa
     7141 aatacctcag gaacattgtt accaagtatc ggtttaataa tcgaactgta attaataatg
     7201 tgctgcagga attaaataag attttacata atcatcaata c
//
LOCUS       DAORKW010000133         7228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_325_length_7228_cov_4.116688, whole genome shotgun sequence.
ACCESSION   DAORKW010000133 DAORKW010000000
VERSION     DAORKW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_325_length_7228_cov_4.116688"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLB46_06855"
     CDS             <1..727
                     /locus_tag="PLB46_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76272.1"
                     /translation="YIQPHQPIIAESSVELLQSETWFFINQCKIFAAHQFNIEEKTLH
                     IPDAFLRHHEQHDLNRKSVDVWYQFLKEHYGEIQSYYIQVKMHELTAIQVKELDQLMA
                     ASRSGMHAVKCIHDVSHDLRELRNSAHEAKYNFLLHMNKNQTEFYNQLYFDNTTLQDA
                     EVVYDKLVEYLKGVQLAYNFNLQMIYTLSANSSLSDVEIATLMNFNREIFTGNKSLIM
                     SWKNYLLPTDLSAKFSDLPTYMA"
     gene            complement(968..2689)
                     /locus_tag="PLB46_06860"
     CDS             complement(968..2689)
                     /locus_tag="PLB46_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF76273.1"
                     /translation="MTQQTIKTNYSALSTLVTVFFFWGFIASGNGVFIPFCKNYFHLD
                     QFQSQLIDFAFYGAYYLGALFLFLVSVLRKSDLVSGWGYKRSIVYGLLFSFLGSLAMI
                     AAVNFGGFSGILVSLFIVALGFSLQQTAAQPFAITLGDPATGNTRITLGGAVNSFGTT
                     IGPIVVGLALFGSVAVTDDMINSLELDSITLLYCGIGALFVVCALMFFMSKKVPDGKL
                     DAHVEPANHALTTLIGITAALAIVFTVVFSSYTTTEARQVETMRTELAAIDANDTVAL
                     AAKNEAISKLNAPIENKRMVLLGSALVVVVGGLLFAKSRAAKKQEGWGALHYPQLVLG
                     MIGIFTYVGVEVTIQSNLSELLKQPSFGGLQAAEIAPYISMYWGSLMIGRWAGSIAAF
                     SVSGMQKKLLMILVPIIAFGVIIGVNSLANHDMRPLYGYIVCIAIQIAAFFYGKDKPA
                     RTLFIFSALGVLAMLIGIMTTGTIAVFAFLSGGLFCSIMWPNIFALSVAGLGKYTSQG
                     SAFLIMMILGGGIIPPIQGKLADIIGIHPSYWVTVLCFGYLAWFAIAVSKILKSQGVA
                     TGEAGAH"
     gene            complement(2838..3338)
                     /locus_tag="PLB46_06865"
     CDS             complement(2838..3338)
                     /locus_tag="PLB46_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76274.1"
                     /translation="MKKLLFVICIAPMVLLITGCPVGITYPLGTPGTEKIDKSILGKW
                     GQEPGDDKEILSVQIEKKDDHSIKVTVLERGPMYMEEVDDFNGWFTEINDMQFVYFQN
                     AADANGTYYHYAYKIEGDNLVTYDISLLDGGTDAVVSTEAFREQVKLSMQRPDFLSSK
                     TVWTKL"
     gene            4609..4902
                     /locus_tag="PLB46_06870"
     CDS             4609..4902
                     /locus_tag="PLB46_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76275.1"
                     /translation="MLPVIGKFKQRYQLEKLVIVANAGLLSKHNIEELLSKRYEFILG
                     GRIKSERRELKQKILSNVFSDGESIVRGSDEHTELIVSYASLRSTKDEQNRSR"
     gene            4987..5142
                     /locus_tag="PLB46_06875"
     CDS             4987..5142
                     /locus_tag="PLB46_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76276.1"
                     /translation="MVGDIQINIDYDKFKVDARWDGLKGYIAYCKMNKDLIIENYKHL
                     WQIENAF"
     gene            complement(5476..6003)
                     /locus_tag="PLB46_06880"
     CDS             complement(5476..6003)
                     /locus_tag="PLB46_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76277.1"
                     /translation="MKKIFTVFVTTLGFLFSTDLVGQCRFEKNEIDDFNKTKTIQTEK
                     ERINSIGHMYMQLQFQYVGNKYFLMLKTNQEDIKFYVTNGQQLIFLFTDGTTLTLDCY
                     SSDWDSETILGTYYEVYYMYYPLSATQMENFKSKEIEKYRLFTSEGTYDTPFNSKKDK
                     DVKKLAECIIEEVGN"
     gene            complement(6024..6527)
                     /locus_tag="PLB46_06885"
     CDS             complement(6024..6527)
                     /locus_tag="PLB46_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HRF76278.1"
                     /translation="MKALFTLVILLISVQIFGQISFKGNFDLNSQTYSSYNNNTQIAV
                     GIGIEKPIGYKVSAAFDIDLWLGGQTLLYIDGVFKYHFMEYANKLYTGFYAGYGILQE
                     DAWYLNFGAEVGYDVPISENLVIQPHGRLGFNSINYGNPAYTTIIIASDAGLHYGIGI
                     SAIWLFE"
BASE COUNT         2489 a         1223 c         1273 g         2243 t
ORIGIN      
        1 atacatacaa ccccatcaac cgattattgc tgaatcttct gtagaattat tacaatcaga
       61 aacctggttt tttattaatc aatgtaaaat ttttgcggcc catcagttta atattgaaga
      121 aaaaacatta catattccgg atgcgttttt gcgccaccat gagcagcatg atttaaacag
      181 aaaatcggtg gatgtctggt atcaattttt gaaggaacat tacggtgaaa ttcaatcgta
      241 ttatatacag gtaaaaatgc atgaattaac tgctatacaa gtaaaagaat tagaccagtt
      301 aatggcggct tcaagaagtg gtatgcatgc cgtaaagtgt attcatgatg tgtcgcatga
      361 tttacgtgaa ttacgcaatt cggcacatga ggcgaagtat aattttttgt tgcacatgaa
      421 taaaaatcaa acggaatttt ataaccaact gtatttcgat aatacaacgt tgcaagatgc
      481 agaagtggtg tatgataaat tggtggaata tttaaaagga gtgcagcttg cgtataattt
      541 taacttgcaa atgatttata cattatcggc aaatagtagc ttatcagatg tagaaattgc
      601 aacgctgatg aattttaaca gagaaatatt tacaggaaat aaatcattaa tcatgagttg
      661 gaaaaattat ttattgccaa cagatttatc tgccaaattc agtgatttac cgacttatat
      721 ggcataaata tttttagtcc gcaactgctg gaacagccga aaccacggag aaccacagag
      781 aaaatacaca cagagctcac ggagaatttt ttcgtaagtt taaattaagc gctatggttg
      841 cgcattaaaa ataaaataaa aactatttta ttaacccctc tgtatttttt cttttggctg
      901 tttctttgaa atcccaatag ctatcgggat tgtggttttg gctgtttgta aacgaatatg
      961 tttttattta atgcgcacct gcttcaccgg tagcaacacc ttgtgatttt aatattttgg
     1021 aaacagcgat ggcaaaccat gctaaatatc caaaacataa aacagttacc caatacgaag
     1081 gatgtatacc aattatatct gcaagttttc cttgaattgg cggaataata ccaccaccta
     1141 aaatcatcat gattaaaaat gcagaacctt gtgaagtata ttttcctaaa cccgcaactg
     1201 ataatgcaaa aatgttcggc cacataatac tgcaaaataa tccaccactt aaaaatgcaa
     1261 ataccgcaat ggtacccgtt gtcataatgc caattagcat ggctaaaaca ccaagtgctg
     1321 aaaaaataaa taacgttctt gcaggtttat ctttaccata aaaaaatgct gctatttgta
     1381 tagcaataca aacaatataa ccgtataaag gacgcatgtc gtggttcgct aatgagttaa
     1441 ctccgatgat aacaccaaat gcaataatcg gcaccaaaat cattaataat tttttctgca
     1501 tacccgacac cgaaaatgct gcaatggaac cggcccagcg accaatcatt aaacttcccc
     1561 aatacataga aatatagggt gcaatttctg ctgcttgtaa tccaccaaat gaaggttgtt
     1621 ttaataattc agataaatta ctttgaatag taacttcaac accaacatag gtgaaaatgc
     1681 caatcatccc taatactaat tgaggataat gtaaagctcc ccagccttcc tgtttttttg
     1741 ctgcgcggga ttttgcaaat aataatccgc caacaactac aactaatgca ctgcctaata
     1801 aaaccatacg tttgttttca ataggtgcat tgagttttga tatcgcttcg tttttagctg
     1861 ctaaagcaac agtgtcattg gcatcaattg ctgctaattc ggtgcgcatt gtttctacct
     1921 gacgagcttc tgttgttgtg tagctactaa atacaacagt aaaaacgatg gccaatgcag
     1981 ctgttatgcc tattaaagtt gtaagtgcat gatttgctgg ttctacatgc gcatccagtt
     2041 tgccatctgg aacttttttc gacataaaaa acatgagtgc gcacacaaca aataacgccc
     2101 cgataccaca atatagtaaa gtaattgaat cgagttccaa tgaattaatc atgtcgtcgg
     2161 taactgcaac cgaaccgaat aatgccaaac cgacaacgat aggaccaata gtagttccaa
     2221 atgaattcac tgcaccacct aaagttatac gggtatttcc agtagcaggg tcacccaatg
     2281 tgatggcaaa tggttgggca gcagtttgct gtaacgaaaa tcccaatgca acgataaata
     2341 aactcactaa aatcccggaa aatccgccaa agttaacggc agctatcata gctaaggagc
     2401 ctaaaaatga aaacaacaaa ccatatacga tactgcgttt atatccccat ccggaaacca
     2461 aatcgctttt gcgtaaaaca cttactaaaa ataaaaacag cgcccctaaa taataagcac
     2521 cataaaaagc aaaatcgata agctgtgact gaaactggtc taaatggaag taatttttac
     2581 aaaacggaat aaacaccccg ttcccggaag ctatgaaccc ccagaaaaag aataccgtaa
     2641 caagcgttga aagtgcgctg taattggttt taattgtttg ttgtgtcata atagtaagtg
     2701 ttttgcggta atgcctgaat ggcttatcct atgcaaataa gtaaataatt tggcatatca
     2761 ccaaacaatg agggggtact tattgaaaaa attacaataa gtatcaaaca ataggatttt
     2821 cgagggttat ggacttttta taacttggtc cagacagttt tgctgcttaa aaaatccggg
     2881 cgctgcatcg ataatttgac ttgttcccta aatgcttctg tggaaacaac cgcatcggta
     2941 ccgccatcca ataatgagat atcgtaagta actaaattat ctccttctat tttatatgca
     3001 taatggtaat aggtgccatt ggcgtcggcg gcattctgga aataaacaaa ttgcatatca
     3061 ttgatttctg taaaccaacc attaaagtca tctacttctt ccatgtacat tggtcctcgt
     3121 tctaagactg taacttttat tgaatggtca tctttttttt ctatctgtac acttagaatt
     3181 tctttatcat caccaggctc ctgtccccat ttacctaaaa tggatttatc aattttttca
     3241 gtaccgggtg tgcccagtgg ataagtaatt cctacagggc atccggtaat taataatacc
     3301 attggtgcaa tgcatataac aaataacagc tttttcatat cgtggtgttt attaaacaaa
     3361 ggtacatttt taagcacatt tagttcactt aataatcctt taaattaacc tagatgcgtc
     3421 ggaaagaaaa ggtcggaatt ggtgcacgac agttttagac agacatagca gtcaaaggtt
     3481 gggaactcaa atgtaaggaa aggcaattgg tcattattta gaattgctaa aaagaacact
     3541 caaatttgtt gttttattga aataatgata cattcgctac taattaggta ccaaacctac
     3601 ccaaaacata caccatatcc tcccggttac ccaacgagtg ttaggttgta agtcatggta
     3661 agtttgtagc aagcacaaca atgttggttg gtattttaac attcaaaacc attaccttgt
     3721 gtgcgaatta attgagtaca actaatcatt atacattcaa ctaacaacaa aaacacgcta
     3781 aatgaaaatc taaagattaa ttctgttttc cacagttttg taaaaacgag ttggaatgtc
     3841 ggctgtaacc cttgattttg gtaggttgat gttaaaaagt tgaatggctc gtttttaatt
     3901 tgggttgtgt ttacaaggca aagtacaaat aaaagtggaa gcatcagaat taaggtggta
     3961 gccaccagta gttataaaga taggattata agaaccattg gaagccactg ctatgctgac
     4021 tatgtgaagg ttttgcggaa tcaaagttta gattttgtag accaatataa gcacatacga
     4081 acgctcaaat ttattcattc tgaggatgct tctttttttt cgaaagtaaa acagggatta
     4141 aagcaggtcg aaacgattgt gcgggcttta atcctaggta aattatttga ccaaattgga
     4201 tttaatatca ttgaagaaga acttttctga cacttggtaa taagccgatt ggtttttcca
     4261 ttaggtaaat taacaacacg aatatttact gcagtataaa tcgctgcata ttgaagcaga
     4321 gcagctatat caattctttg aaaagctgta ttttaaatac aaggatattg ttaaaaatac
     4381 aagttatacg cacaagttaa atatacagac gtgtgaaatg agtattgtat tttacgacgt
     4441 aactaaaatg tatcttgaaa ccaatgatga agatgacttg agaaggtccg gtttttcgaa
     4501 ggagggcaaa catcaaaacc cccaaatcgt tttggggtac tagtaagtct aaacggctat
     4561 ccattagcat atgaagtgtt tgagggtaat aaatttgaag gtcaaacaat gcttcctgtc
     4621 ataggaaaat ttaaacaacg ttatcaatta gagaaacttg ttattgttgc caacgcaggt
     4681 ttgctatcta agcataatat tgaagaatta ttaagtaaac gatatgaatt tattttaggt
     4741 gggagaatca aaagtgaacg aagggagcta aagcaaaaaa tattgtcgaa tgtgtttagt
     4801 gatggggaaa gcattgtgag aggtagtgat gagcatactg aattaattgt tagttatgct
     4861 agtttgaggt caacaaaaga cgagcaaaat agatcccgtt gattataaaa attagaggcg
     4921 tcattaagaa atgggaaatt aggtaaacaa catatcaata accgtggatg caataaatac
     4981 ctgaggatgg tgggtgatat ccaaattaat attgattacg ataaatttaa ggtcgatgca
     5041 cgatgggatg gattaaaagg atatatagcc tactgtaaaa tgaataaaga cctaatcatt
     5101 gaaaactata agcatttatg gcaaatagaa aacgccttct aaatatccaa aacatattta
     5161 cgcatccgcc cggtgtatca caggttagaa cagcgaattg aagtgcacgt ctgcatagca
     5221 ttctgctctt ataaattgta ttaagaattg taacgacaat taatgtttgg tagaaccaag
     5281 atcagtgtcg aaaaagcaat agatctaata aaaactattt accaaatatc aatcgtattt
     5341 catgaataga cacaaccaag caaattcata tttgcatcgc agcatgaaca aattctccta
     5401 cttaaggctt ttgaaaatga aatataaatc cgactggctc gttgcgataa aaagaagggt
     5461 aattaacttt tttcttcaat tcccgacctc ttcaatgatg cactcagcta atttttttac
     5521 atctttgtcc ttttttgaat tgaaaggggt gtcatatgtt ccctcagatg tgaataatct
     5581 atatttttca atttccttgg atttaaaatt ttccatttga gttgcactta aaggataata
     5641 catataatat acttcataat aagtgcctaa tatagtttct gaatcccaat ctgaagaata
     5701 acaatccaat gttaatgtag ttccatcggt aaacaggaat attagttgtt ggccattcgt
     5761 tacataaaat tttatatctt cttgatttgt ttttaacatt aagaagtatt tattcccaac
     5821 atattgaaat tgcagctgca tatacatatg tccaatacta ttaattcttt ccttttcagt
     5881 ttgaattgtt tttgttttat taaaatcatc aatttcattc ttttcaaacc tacattgacc
     5941 aactaaatct gtggaaaaaa gaaagcccaa agttgttaca aatactgtaa agattttttt
     6001 cataatgctt gatttattat taactattca aatagccata ttgcacttat accaatacca
     6061 taatgcaatc cagcatctga ggctatgata atagttgtat aggccggatt gccgtagttt
     6121 attgaattga atcctaacct tccatgtggt tgtattacga gattctcaga aattggaacg
     6181 tcataaccaa cctcagcgcc gaaatttaaa taccaagcat cttcctgcaa tattccatat
     6241 cctgcataaa atccagtata aagtttgttt gcatattcca taaaatgata tttaaaaaca
     6301 ccatcaatat agagaagagt ttgtcctcca agccacaaat caatatcgaa agctgccgaa
     6361 actttataac ctattggctt ttcaatgcca ataccaactg ctatttgggt gttgttgtta
     6421 taagatgaat aggtctgaga atttaaatca aaatttcctt taaatgatat ttggccaaaa
     6481 atttgcactg aaattagtag aataacaagt gtgaacagag ctttcatttc gtaggtttat
     6541 gcagcaaata tattataaat ttattatttt tgctaattaa ttagttaagg taaaattatc
     6601 taatatattt tggtgaaatt gagattgcag gggttattgg ggcacaatac aattaatgcg
     6661 aagattgtaa ttttcatata acatgggttt gaagattgaa cctagcgtaa tcccctctct
     6721 tttaaagtga gtgcttttgg atttgaagca aatataatca taattctgaa aaatttaatg
     6781 catttccatg aggacagagt agtggttgtt gtagctattg agcaaatagg taaataaatc
     6841 aattgtgttg attatcaagt agatatgtca agaaggcata taattattac catacctctt
     6901 ttctttagtt agtaactttg attcggctac aacggatttc cctttggctt tgtaatagag
     6961 tatttaccaa atacatttac tgccaaaaca gctagaataa ggacaaattt accttttggc
     7021 ttcctgatag gggggcagca aggctagttg cttgcaagac tggaatttac aagtccaatg
     7081 gactaaaaga ttcggtaata cattaagggg ttcaaagtgt tgttagaaat tgaatgccaa
     7141 tattcaaagc aaaacacaca ttgctttcgt tttgaccggc attaaattaa tgggtgaggt
     7201 atgtgaaatt atatcctttg gctttaag
//
LOCUS       DAORKW010000134         7199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_328_length_7199_cov_3.973684, whole genome shotgun sequence.
ACCESSION   DAORKW010000134 DAORKW010000000
VERSION     DAORKW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_328_length_7199_cov_3.973684"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2839
                     /gene="gcvP"
                     /locus_tag="PLB46_06890"
     CDS             <1..2839
                     /gene="gcvP"
                     /locus_tag="PLB46_06890"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRF76279.1"
                     /translation="PRPHDVAAMLTSIGAESLDSLINQTVPENIRLKRPLNISQPMTE
                     HNYFKHLKAIAAKNKVFRTYIGLGYYNTLTPSVIKRNIFENPGWYTQYTPYQAEIAQG
                     RLEALLNFQTMIADLTGFDIANASLLDEGTAAAEAMIMFFHLRNKKEVVANKFFVSEL
                     CFPQTIDLLISKAEPLGIEVEVGNHTTFTFNSNYFGALLQYPAGNGQLYNYRSLIDDA
                     HQHNAYVTVAADILSLAILQSPAEMGADCAIGNSQRFGVPMGFGGPHAAFFATKDEFK
                     RDIPGRIIGVSVDRDNNPALRMALQTREQHIRREKATSNICTAQALLAIMAGMYGVYH
                     GPEGLKQIAEKVHGLTSVLNDTLNKMGFVQSNTAFFDTLYIKAGAMSGVIHQHAISRH
                     MNFFYHMNGDIQITLDESTELEDIKEIAEVFAEAKGSNNGHKIELSNIENLQTTIPAE
                     LIRTSSYLTHPVFNTYHSESEMMRYIKRLENKDLSLTTSMIPLGSCTMKLNAATELMP
                     VSWPEFSSIHPFVPAEQVQGYLEMIKTLEDDLCEITRFDACSLQPNSGAQGEYAGLMV
                     IRAYHRHHNEAHRNKVLIPASAHGTNPASAVMAGMDVIVVKSDAEGHIDIDDLKLKAE
                     EHQNDLACLMVTYPSTHGVFEETIMLICNIVHQHGGLVYMDGANMNAQVGLTSPAKIG
                     ADVCHLNLHKTFAIPHGGGGPGMGPICVNEKLAPFLPGHPLFKTGGINAIRAINAAPF
                     GSASILLISYAYIKMLGGEGCTNATKMAILNANYLKSNLEKHYDILYVSKSGRVAHEF
                     IIDFRKWKHTIGLEVEDVAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKEELDRFIE
                     AMVAIKAEIAAIENGDADKVDNTLKNAPHTMRDILSESWNHGYSREQAVYPVSYLKDR
                     KFWPSVARINNTQGDRNLICTCPPIESYMEV"
     assembly_gap    3089..3098
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3099..3588
                     /locus_tag="PLB46_06895"
     CDS             <3099..3588
                     /locus_tag="PLB46_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76280.1"
                     /translation="VLRLSSGTSSLYLEYHFALTLLKPSESEYYALSMGGYEFTDSAL
                     VSERVSSWAIGLNITYLYGADVWDNFRIGARFGLDYVRETIDQTATDAGQFYEASTPV
                     SGPKVGIGLSSLYYITEHFSINGDLYYHASRQGFNALNPLDPKLQLNNSINLNLGASF
                     YF"
     gene            3658..4011
                     /locus_tag="PLB46_06900"
     CDS             3658..4011
                     /locus_tag="PLB46_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF76281.1"
                     /translation="MENLKPLVDDLNARILAGDILGAFEMYYADDVVMQDNNFPVRTG
                     KDECRKFEQDFVSKLTAFRGAQLHNVLISDGIVINEWEFDYTHSEWGDRKYKQVSVQR
                     WNDGKIVEEKFYYNN"
     gene            4117..5091
                     /locus_tag="PLB46_06905"
     CDS             4117..5091
                     /locus_tag="PLB46_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HRF76282.1"
                     /translation="MNKGPISNFIQHHYRHFNAAALVDAAKGYEQHLLEGGKMMVTLA
                     GAMSTGELGISFAEMIRQGKVDIISCTGANLEEDIMNLVAHSHYKRVPNYRDLTPEQE
                     WELLEQGLNRVTDTCIPEEEAFRKIQQHIVKIWMDAEAAGERYFPHEYMYKLLLSGVM
                     KEGYEIDPKNSWMIAAAEKNIPIVVPGWEDSTMGNIFASYCIKGELKASTMKSGIEYM
                     VWLSDWYRNNSAGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWSYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLDIHTPKFIVESDATIVAPLIFAWVLGW"
     gene            5196..6485
                     /locus_tag="PLB46_06910"
     CDS             5196..6485
                     /locus_tag="PLB46_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HRF76283.1"
                     /translation="MYTTPHYISADEALSCIKSGDRVFVHGSACTPTHLLKHLAQRSN
                     TLTDVELVFISVYGDMYVDQPEYRDSFRMNSMFVSNSIRSDVNSGFADYIPVFLSEIP
                     ELFKRGILTLDVALVHVSPPDKHGYCSLGVSVDIARTAVNTAKYVIAQVNPQVPRTHG
                     DSLIHSSRFHSMVYVEEPLHEASFGERVGEAELQIAQHIAGLIEDRSTLQMGIGAIPD
                     AVLKALTNHKDLGVHTEMFSDGLIPLFDADIVNNKYKKIHPNTTVSGFALGSKKLYDY
                     VDDNPAFEFLDIDYVNEPSIIKQNDKVVAINSAIEVDITGQVCADSIGTIQYSGVGGQ
                     MDFMRGAALSEGGKPIIALSSRTAKGISRIVPVLKPGAGVVTTRAHVHYLVTEYGVAY
                     LFGKNLRQRAKALIEIAHPDDRAMLHQSCFERFKVFV"
     gene            complement(6652..>7199)
                     /locus_tag="PLB46_06915"
     CDS             complement(6652..>7199)
                     /locus_tag="PLB46_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76284.1"
                     /translation="YLTDPEAVMPISKLLSPGYLTAAVGLDWKPSEKFSLFLSPATGK
                     FTFVMDDVIASTVTDTVNNKNRYGVDAAENLRAEFGASMVAALNTPIGKNTSLSSKLT
                     LFNNYTDPITEHRGNIDVDFQNNLNIKISKYFATTIFLQVLYDHDIAIATYNDDDVQI
                     GTGPKTQFKEVFGIGLSYSFQ"
BASE COUNT         2244 a         1191 c         1515 g         2239 t
ORIGIN      
        1 cccaagacca catgatgttg cagcaatgtt aaccagtatt ggagctgagt cgcttgactc
       61 gctcatcaac caaacagttc cggaaaacat ccgcctgaag cgaccgttga atatcagtca
      121 gcccatgacc gaacacaatt acttcaaaca cctaaaggca attgccgcta aaaacaaggt
      181 gtttaggaca tatatcgggt tagggtatta taatacgctc accccaagtg taattaagcg
      241 caacatattc gaaaatccgg gttggtatac gcagtatacc ccttatcagg ctgaaattgc
      301 ccagggcagg ttagaagcgt tgctaaattt tcaaaccatg attgccgatt taaccggatt
      361 tgatattgcc aacgccagtt tattggatga aggaactgca gctgctgaag caatgatcat
      421 gttttttcat ttgcgcaata aaaaagaagt agttgccaat aagttttttg taagtgaatt
      481 gtgtttccct caaacaatag atttattaat tagcaaagct gaaccacttg gtattgaagt
      541 tgaagttggg aatcatacaa catttacttt caacagcaac tattttggag ctttgttaca
      601 atatcctgcc ggaaacggac aattatataa ctatcgttca ttaattgatg atgcacatca
      661 acacaatgct tatgtaacag tagctgcaga tattttatca ttggcaattt tacaatcacc
      721 tgccgaaatg ggtgcagatt gtgctattgg taattcgcaa cgttttggag taccaatggg
      781 ttttggtggt ccgcatgcag cattttttgc aactaaagat gaatttaaac gcgatattcc
      841 gggacgtatt attggggtga gtgttgaccg cgataataac cctgcgttgc gtatggcatt
      901 acaaaccaga gagcaacata tcagaagaga aaaagcgaca tccaacattt gtactgcaca
      961 ggctttactt gccattatgg ccggtatgta tggtgtttat cacgggcctg agggtttaaa
     1021 acaaattgca gaaaaagtac atggtttaac cagtgtatta aatgatacgc tgaataaaat
     1081 gggttttgtg caatcaaata ctgctttttt tgatacgttg tatattaaag caggtgcaat
     1141 gagtggtgta attcatcagc atgcaatcag cagacatatg aatttctttt atcatatgaa
     1201 tggcgatata caaattaccc ttgatgaaag tactgaactt gaagatatta aagaaattgc
     1261 agaggtattt gcagaagcaa aaggttcgaa caacggacac aaaattgagt taagtaatat
     1321 tgaaaatttg cagactacaa taccggcaga attaattcgc acatcatcct atttaacgca
     1381 tccggtattt aacacctatc attctgaaag tgaaatgatg cggtatatta aacgtttgga
     1441 aaataaagat ttatcgttga caacatcaat gattccattg ggaagttgta caatgaaatt
     1501 aaatgcggca actgaattaa tgccggtttc atggcctgaa ttttcttcca ttcatccatt
     1561 tgttccagcc gaacaggtgc aaggatattt agaaatgatt aaaacgttgg aagatgattt
     1621 atgtgaaatc acccgttttg atgcatgcag cctacagcct aattcaggtg cacagggcga
     1681 atatgctggg ttgatggtaa ttcgtgctta ccacagacat cataacgaag cacatagaaa
     1741 taaagtatta attccagcaa gtgcacatgg tacaaatcct gcaagtgcgg ttatggccgg
     1801 aatggacgtt atcgtagtaa aaagtgatgc agaaggacat atcgatattg atgatttaaa
     1861 attaaaagcg gaagaacatc aaaacgactt agcatgtttg atggtaacgt atccatctac
     1921 acatggtgtg tttgaagaaa ccattatgtt gatatgtaat atcgtacatc agcatggcgg
     1981 attggtatat atggatggcg ccaatatgaa tgcacaggta ggcttaactt ctcctgcaaa
     2041 aattggtgcg gatgtttgtc acctcaactt acataaaaca tttgccatac cgcatggtgg
     2101 tggtggaccg ggaatgggac caatttgtgt gaatgaaaaa cttgctccgt ttttacccgg
     2161 acatccatta tttaaaacag gtggtataaa tgccattcgt gctattaatg ctgcaccatt
     2221 tggcagtgca agtatattat taattagtta tgcgtatatt aaaatgttgg gtggtgaagg
     2281 atgtactaat gcaacaaaaa tggccatctt aaacgcaaat tatttaaaga gtaatctcga
     2341 aaaacattac gacattttat atgtgagtaa atcaggcaga gtagcacatg aatttattat
     2401 tgatttcaga aaatggaaac ataccatcgg attagaagtt gaagatgttg cgaaacgatt
     2461 aatggattat ggtttccatg cacctactgt ttcgtttcct gttgccggta cgttaatgat
     2521 tgaacctacc gaaagtgaaa gcaaggaaga attagacaga tttattgaag ctatggtggc
     2581 tattaaagca gaaatcgcgg caatagaaaa cggggatgca gataaagttg ataatacctt
     2641 aaaaaatgca ccacatacca tgcgcgatat tttgtcagaa agctggaatc atggttattc
     2701 gcgagagcag gctgtttatc cggttagtta tttaaaagac agaaaattct ggccaagtgt
     2761 tgccagaatt aataatactc agggcgacag aaatttaatt tgtacctgtc ctccaattga
     2821 aagttacatg gaagtttaaa cacttctaac tagatttatt caaggctgag ttggatttat
     2881 catagtcaat tatttacttt acagtaaaat aattaatatg aaaacaccaa ttcagctttt
     2941 tttattgagt ttatttattt ccagtgtgtt acaaagccag gtttatgttg gcgcacaagg
     3001 aacggtgcaa aaccaatttg cctttcaatc ttttgatctt gaaagtgtag tgcccggtat
     3061 tgtggctaga gtaggttatt ttgattggnn nnnnnnnncg tgctgcgtct gtcctctggc
     3121 acttcttcct tgtatttgga ataccatttt gcgttaacgc ttttaaaacc ttccgaatca
     3181 gaatattatg cgttatcgat gggtggatat gagttcaccg acagtgcatt ggtaagtgaa
     3241 agagtgtctt cttgggcaat aggactgaat attacttatt tatacggcgc cgatgtttgg
     3301 gataattttc ggataggggc gagatttggg cttgactacg ttcgcgaaac tatagaccaa
     3361 acagccacag atgccggaca attttatgaa gcatcaacac ctgtaagtgg accaaaagtt
     3421 ggtattggtt tatcatcact gtattatata acggagcatt tttcgattaa tggcgactta
     3481 tattaccatg catctagaca aggttttaat gctttaaatc cgttggaccc caagctgcaa
     3541 ttgaataata gcatcaacct gaatctcgga gctagtttct acttttaatt gacaattttt
     3601 tccgcttact gaacttttta tccatttata tgtatataca tgtaaattga tgtaaaaatg
     3661 gaaaacttaa aacctttagt agacgacctg aatgcccgca tattagccgg tgatatatta
     3721 ggtgcttttg aaatgtatta cgccgatgat gtggtaatgc aggacaacaa tttcccggtt
     3781 agaactggaa aagacgaatg tcgcaaattt gaacaggact ttgtaagtaa actcaccgct
     3841 tttcgtggtg cacaattgca caatgtttta ataagtgatg gaattgttat caatgaatgg
     3901 gaatttgatt atacgcatag tgaatggggt gatagaaaat ataaacaagt atctgttcag
     3961 cgttggaatg atggtaaaat tgtagaagaa aaattttact acaacaatta attgatcaag
     4021 atataaatac gcccgttctg ctaatcggca aacgggcttt ttttatgtcc ggaagcgcca
     4081 ttttttttgt tactttgcag ttcaaaatat tgaacaatga acaaaggacc tatttcgaat
     4141 tttatacagc accactatcg ccactttaac gcagccgcat tggttgatgc tgcaaaaggg
     4201 tatgagcaac atttgctgga aggtggcaaa atgatggtaa cccttgccgg cgctatgagt
     4261 acgggtgagt taggtatttc atttgctgaa atgattcgtc agggtaaagt agatattatt
     4321 agctgcacag gagccaatct tgaggaagat atcatgaatc tggttgcaca ttctcattac
     4381 aaacgcgttc ccaattaccg cgacctgact ccggagcagg aatgggaatt gttggaacaa
     4441 ggtttaaatc gtgtaactga tacctgtatt ccagaagaag aggcttttcg taaaattcag
     4501 caacatattg taaaaatatg gatggatgcc gaagctgcag gtgaacgtta tttcccacat
     4561 gagtatatgt ataaattgtt attgagtggt gtcatgaagg aaggatatga aattgatcct
     4621 aaaaattcat ggatgatagc tgctgcggaa aaaaatattc cgattgtagt tccgggatgg
     4681 gaggatagca ctatgggaaa tatttttgct tcctattgta ttaaaggcga attaaaagct
     4741 tctaccatga aaagtggtat cgaatatatg gtttggttaa gcgattggta tagaaataat
     4801 tcggcaggca aaggtgttgg atttttccaa attggcggtg gtattgcagg tgatttccca
     4861 atttgtgtag tgcctatgat gtatcaggat ttggaatggc atgatgttcc attctggagt
     4921 tatttctgtc agatatctga ttctaccact tcatacggta gttattcggg tgccgttcca
     4981 aacgaaaaaa ttacctgggg aaaattagat atccatacac ctaagtttat tgtagaaagt
     5041 gatgccacta ttgttgcccc attaattttt gcctgggtgc tcggttggtg atttttatca
     5101 actttcttgt tagattacct gtctaatttt gcggtattaa ttaaaatcaa ttggcaataa
     5161 tcaatattgc cgcttgtcat caataattta taattatgta tactacaccg cattatattt
     5221 cagccgatga agcacttagt tgtattaaat cgggcgaccg ggtttttgtg catggaagtg
     5281 catgcacacc tacacattta ttaaaacatc ttgcccaacg cagtaataca ttaaccgatg
     5341 ttgaattggt atttatcagt gtgtatggcg atatgtatgt tgaccaaccg gaataccgcg
     5401 atagttttcg tatgaattcg atgtttgtgt ctaacagtat tcgttcagat gtaaacagtg
     5461 gttttgcaga ttacataccg gtatttttaa gcgaaattcc cgaattattt aaaagaggaa
     5521 ttttaacctt agatgtagca ttggtgcacg tttctcctcc cgacaaacat ggttattgtt
     5581 cattaggtgt ttctgttgat attgccagaa ctgccgtgaa tacagctaaa tacgtaattg
     5641 ctcaggttaa tccacaagtg ccaagaacgc atggtgatag tttgatacac tcatcgcgtt
     5701 ttcattctat ggtgtatgta gaagaacctt tacatgaagc atcatttggt gaaagggtag
     5761 gagaagctga attacaaatt gcgcaacata ttgccggatt aatagaggac agaagtactt
     5821 tacaaatggg tattggcgcc attccggatg ctgtattaaa agcacttacc aatcacaaag
     5881 atttaggcgt gcataccgaa atgttttcag atggtttaat tccgcttttt gatgctgata
     5941 ttgtcaataa taaatacaaa aaaattcatc caaatacaac agtatccgga tttgcattag
     6001 gatcaaaaaa attgtatgat tatgtagatg ataatccggc atttgaattt ttggatatcg
     6061 attacgtaaa tgaaccttcc attatcaaac aaaacgataa agtagttgcc attaacagcg
     6121 ctatagaagt agatattacc ggtcaggttt gtgcggatag tattggcact attcaatatt
     6181 ctggtgttgg cgggcaaatg gatttcatgc gtggagcggc attaagtgaa ggaggtaaac
     6241 caattattgc attgtcgtca cgtacggcaa aaggtatttc acgaatagtt cctgttttaa
     6301 aacctggtgc aggtgttgtt acaacaagag ctcatgtgca ttatttagta actgaatacg
     6361 gtgtagctta tttgttcgga aaaaatttgc gtcaacgagc aaaagcatta attgaaattg
     6421 ctcatcctga cgacagagcc atgttgcacc aatcttgttt cgaaagattt aaagtttttg
     6481 tttaatccag ctaatagtac attttcgaaa taggttatat tattatagca gcgccattta
     6541 agtaattaat taaatggcgc tgttttattt tggggtatta tttgaatgaa tacaaaaaaa
     6601 ataggctatc gtggtgtgac agcctattat aaatgaataa gggttaattt attattggaa
     6661 cgaataactt aatccaatgc caaacacttc tttaaattgt gttttaggac ctgtgccaat
     6721 ttgcacatca tcatcgttat aagtagcaat tgcgatatcg tggtcgtata atacctgaag
     6781 gaaaatggtt gttgcaaaat atttgctgat tttaatattc aggttatttt gaaaatcaac
     6841 gtcaatattt cctctgtgtt cggtgatagg atcagtgtag ttgttgaata aagttagttt
     6901 agaagataag ctcgtgtttt ttcctattgg tgtatttaat gcagccacca tggaagcacc
     6961 gaattctgca cgtaaatttt cggcagcatc aacaccataa cggtttttgt tatttactgt
     7021 gtcggttaca gtagatgcaa taacatcatc catcacaaaa gtgaatttac ctgtagcagg
     7081 ggagaggaac agtgaaaatt tttcacttgg tttccagtcc aaacctactg cagccgtaag
     7141 gtatccggga cttaacaatt tggatattgg cataactgct tccgggtcgg ttaaataat
//
LOCUS       DAORKW010000135         7155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_330_length_7155_cov_5.090141, whole genome shotgun sequence.
ACCESSION   DAORKW010000135 DAORKW010000000
VERSION     DAORKW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_330_length_7155_cov_5.090141"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="PLB46_06920"
     CDS             complement(<1..1716)
                     /locus_tag="PLB46_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF76285.1"
                     /translation="MSKPLLLDIKNLSTHFQTDNGLVKAVDDISFQIFRGETVGIVGE
                     SGSGKSVTSLSMMRLIPNPPGKIAGGEMWYYEEGKNPVDLIKIADKDMRHYRGNKISM
                     IFQEPMTSLNPVFTCGYQVDEAIILHQKLSKKAAKQRTLELFEKVQLPNPERIYNAYP
                     HELSGGQKQRVMIGMAMSCNPDILVADEPTTALDVTVQKTILELMANLKQTINSSIIF
                     ITHDLGVIADIADRVLVMYKGKIVEQGPVAEIFKNPQHPYTKGLMACRPPLGKRLRKL
                     PVIADFMVENEQGELIEKNQTIAGAVEANIISAEETQQRHEQLYLEKPILQIQHIKTY
                     FPTGKSLFGKPTKFVKAVDDVSFDVYPGETLGLVGESGCGKTTLGRTIIRLVEPVSGD
                     IIFKGKSILSLSNQEMKPLRKDIQIIFQDPYSSLNPRMTVGSAIKEPMTVHKLYANDN
                     ERKEKVIELMEKTGLKADFYNRYPHEFSGGQRQRICIARALALNPQFIICDESVSALD
                     VSVQAQVLNLLIQLRNEFKFTYIFISHDLSVVKFMSDRMVVMNKGVIEEMGPADEIYN
                     NPQKAY"
     gene            complement(1799..2455)
                     /locus_tag="PLB46_06925"
     CDS             complement(1799..2455)
                     /locus_tag="PLB46_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF76286.1"
                     /translation="MSEEIFKVSAEQLRQPHGDLAIQVGEKMNEINVHIHRYTFEALN
                     ISANDSILEIGMGNGNFVKDLFKIHPDINYVGCDFSEKMVEESTKNNAQLIAAGKVAF
                     HFANAAKLPLHDAAVNKIFTINTLYFWENPAEVLSELHRILQPKGQLTITIRPKEIMQ
                     HYAFVKYGFTLYAKEDLVDLLNNNHFSVINIVEKNEPDMEVNGEKMPTATLLVTAEKM
                     "
     gene            complement(2483..3940)
                     /locus_tag="PLB46_06930"
     CDS             complement(2483..3940)
                     /locus_tag="PLB46_06930"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HRF76287.1"
                     /translation="MNKLITYCILCLFLIIGIPDSSSAQITGNAVRVEGNMVVVKIKQ
                     RSPDYKKLMMYFGLDEDSLFIHHSLGQLGKEGWKIRKITKRKAIITKVVGDDNMEVQW
                     GNQPIFFNLNVNESITPGYPGPVSYGVNNFKGAPTVYENDQDITVFKLLNFPQASKVF
                     LSGNFNDWSTGQTPMQKTDSGWIAEVKLKPGKYFYKFIIDGQWMQDINNNLRESDGHN
                     NYNSTYYHYNYNFKLEGLTDKKNIILAGSFNNWNERELKMQKTATGWVLPMFLKEGTH
                     TYKFIADGEWILDPGNSAIRPDGNGNINSVITLGDPYNFTLKGYQNANAVVLAGSFNN
                     WNAGELIMQKTDTGWQLPFVLAPGNYEYKFIVDGNWITDPANPVTTKLGETINSFIVI
                     SANHLFTLSAFPTAKEVYITGSFCGWAEPGYKMYNDNGVWKFPCYLPAGKYTYKFIVD
                     GKWMLDPANNNTEQNEFGTGNCVLWVSPESEFLEK"
     gene            complement(3937..4920)
                     /locus_tag="PLB46_06935"
     CDS             complement(3937..4920)
                     /locus_tag="PLB46_06935"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pepsin/retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HRF76288.1"
                     /translation="MKIGSYILALITCILLYGSSIALAGVPPVPDTDKGFDDPIPQGE
                     FDELFIPLKRVQNLFLVEARVDSLIGNFILDTGAPHLVLNKTYFTEGRKAEGEGVSYG
                     ITGGGNTILNTTIDSLIITDLFYTNVDADIVNLGHLEDARGVKILGLLGASLFKEVEM
                     EIDYRNNLLILYKLDKEGNRIVGNERTQLPDVDVSIDITNDIIFMDVVAAEKKLRFCL
                     DTGAERNVISNTLSNKIIKHFQLTTPGGLTGSNGNSQLILNGQMDSVIIGGKSFVAMP
                     FILTNLNYLQLVYDTNINGILGYDYLSKGIVTINHKKKRLTMYFYTAIKTE"
     gene            complement(5003..5422)
                     /locus_tag="PLB46_06940"
     CDS             complement(5003..5422)
                     /locus_tag="PLB46_06940"
                     /inference="COORDINATES: protein motif:HMM:NF022443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HRF76289.1"
                     /translation="MLHLFSGFGLHKQSKWYLLITICLLFIEIAIALFVKDKIIRPYI
                     GDLLVVILIYAFVKIWISNRELLVAAGVFVFACIIELLQYLHIVEVLGLDDNKVATVI
                     IGSKFNTLDIVMYLLGTVVVILAEKFLKSRANKMVDR"
     gene            complement(5465..6829)
                     /locus_tag="PLB46_06945"
     CDS             complement(5465..6829)
                     /locus_tag="PLB46_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76290.1"
                     /translation="MRYSLLFIALLKLQISYAQTGKLALNFGNNGVIITDLQQSADYA
                     TAITMDKSGNVLVAGTTEKGYDAGKQVFVAQYNNKGQLQTNFGKNGKILIPVAHQISI
                     TKIMMQYDGKILIGGTANEKDKTPEFFMLRLLADGTPDKTFGINGLAKSKFGTAYPTV
                     KSNYVLNDFDLDFYNNIIAVGGNNSIMLDQTAIIKFTPSGIIDENFLFEGMYVGSFPI
                     TNSNNKNVINNSCTATKVVALSNGTYLIGGTQYMSNAKSAFQNFTVQRILSNGSADTT
                     FNYGKAFTGGFDEASDELSDIMVNANGNYILVGTTDAGDQDFGCMALNKMGFLDYSAF
                     GDVSKKYGITGKTQTGFAKKGNQEAVSSVMLPDGKFVVVGKGGSIEEEIVIARYLPNG
                     KLDETFGDDGKTVFTPAGKTESDFFDIYEAVLDYEGNLLVVGWLSSKNSGTSADIFIV
                     AFTL"
BASE COUNT         2164 a         1377 c         1217 g         2397 t
ORIGIN      
        1 atatgctttt tgcgggttat tgtagatttc atcggccggt cccatctctt caattactcc
       61 tttattcatt actaccattc ggtcgctcat gaattttacc acgcttaagt cgtgactgat
      121 aaagatatag gtgaacttaa attcattacg taattgaatc agcaaattga gcacctgagc
      181 ctgcacactt acatcaagcg ccgaaacact ttcgtcacaa ataataaatt gtggatttaa
      241 tgctaaagcc cgggcaatac aaatacgctg acgttgacct ccggaaaatt catgtgggta
      301 acgattataa aaatcggctt tgaggcctgt tttttccatc agttctatca ctttttcttt
      361 gcgttcgttg tcgtttgcat ataatttatg caccgtcata ggttctttaa tggcactgcc
      421 aacggtcata cgcggattaa gcgaagaata agggtcctga aaaattattt gaatatcttt
      481 tctcaaaggt ttcatctcct gattggagag cgataaaata cttttccctt taaaaataat
      541 atctcctgaa acaggttcaa ccaaacgaat aatggtgcga cctaatgtag tttttccgca
      601 tccactttca ccaactaatc ctaaagtttc accgggataa acatcaaagg aaacatcatc
      661 cactgctttt acaaatttgg ttggtttacc aaataaactt tttcctgttg ggaaatacgt
      721 tttaatatgt tgaatttgta aaattggttt ttcaagatat agttgttcgt gacgttgttg
      781 tgtttcttca gcactaataa tatttgcctc aacggcacct gcaatagttt gatttttttc
      841 aattaattca ccttgttcat tttccaccat aaaatcggca attacaggta atttccgcaa
      901 acgttttccc aatggtggtc gacaagccat gaggcctttg gtgtaagggt gttgagggtt
      961 tttaaaaatt tctgcaacag gtccttgttc aacaatttta cctttataca ttactaaaac
     1021 cctgtcggca atatcagcaa taacaccgag gtcgtgggtg ataaaaatga tggacgaatt
     1081 aatggtttgt ttgaggttgg ccattaattc cagaattgtt ttttgcacgg taacatccaa
     1141 tgcggtggtt ggctcatcgg caactaaaat atcgggatta caactcatag ccataccaat
     1201 cataacgcgt tgtttttgtc cgccacttaa ctcgtgagga taggcattat atatgcgttc
     1261 gggattcggt aattgcactt tttcaaacaa ttcgagggtg cgttgtttag ctgctttttt
     1321 cgataatttt tggtgtaaaa ttatcgcctc atcaacctga taaccgcaag taaatacggg
     1381 attaaggctg gtcattggtt cctgaaaaat catggagatt ttgtttccac gataatggcg
     1441 catgtcttta tccgctattt taattaaatc gacaggattt tttccttctt cgtaatacca
     1501 catttcacca cctgcaattt ttcccggcgg gttgggaatt aaccgcatca tagaaagaga
     1561 agtcaccgat ttaccagaac ctgattcgcc aactatacca acggtttcgc cacggaatat
     1621 ctgaaatgaa atatcatcaa cagcttttac caacccattg tcggtttgaa aatgcgtaga
     1681 taagtttttt atgtcgagta ataaaggttt agacatgggg taaaaatagg gaaagatttg
     1741 aagatttggt gattggaaag gagcttcatt attgtacttg atgcaaacgt gaaatccatc
     1801 acattttttc cgctgtaacc aatagggtag cagtaggcat tttttcgccg tttacttcca
     1861 tatcaggttc atttttttca acgatattaa ttacagaaaa atgattgtta ttcagtaaat
     1921 ccaccaaatc ttcttttgca tataaggtaa aaccgtattt aacaaatgca taatgttgca
     1981 taatttcctt tggccgaatg gtgatggtta attgcccttt tggttgtaag atcctgtgta
     2041 attcgcttaa tacctctgcc gggttttccc aaaaataaag cgtattgata gtgaatattt
     2101 tattgactgc cgcatcatgt aatggcaatt tggctgcatt tgcaaaatga aatgcgactt
     2161 tgcctgctgc aattaattga gcattgtttt ttgttgattc ttctaccatt ttttctgaaa
     2221 aatcgcaacc tacataatta atgtcaggat ggattttaaa taagtcttta acaaaattac
     2281 cgttgcccat accaatttcc agaatgctgt cgttggcaga aatgttgagt gcttcaaatg
     2341 tatagcggtg aatgtgaaca ttgatttcat tcattttttc acccacctgt atggctaaat
     2401 caccatgtgg ctggcggagt tgttctgcgc ttactttaaa aatttcttca gacatgaaat
     2461 taaattataa aatgaatgaa aattatttct ccaaaaattc agattccgga gaaacccata
     2521 acacacaatt tccggtgccg aattcgtttt gttcagtatt gttattggca gggtctaaca
     2581 tccatttgcc atctacaata aatttataag tgtatttccc ggcgggtaag taacaaggga
     2641 atttccatac accgttgtcg ttatacattt tataacccgg ttcagcccat ccgcaaaaac
     2701 ttccggtgat atacacttct tttgctgtgg gaaaggcaga taatgtaaat agatgatttg
     2761 ctgatatcac aataaatgaa tttatggttt cgcctaattt tgttgtaaca gggtttgccg
     2821 ggtcggtaat ccaattgcca tctacaataa atttatattc ataattgccg ggtgctaata
     2881 caaatggtaa ttgccatcct gtatccgttt tttgcatgat taattcaccg gcattccaat
     2941 tattaaaact acctgctaac acaacagcat tcgcattctg ataacccttt aacgtaaaat
     3001 tataagggtc acctaaagtg ataacggaat ttatattgcc attaccatcc ggtctgattg
     3061 ctgaattgcc agggtctaaa atccattcac catctgcaat aaatttgtag gtatgcgtgc
     3121 cttcttttaa gaacatgggt aacacccaac ctgttgcagt tttctgcatt tttaattctc
     3181 tttcattcca gttattaaaa ctaccggcga gaataatatt ttttttatcg gtaaggcctt
     3241 ccagtttaaa attgtagttg taatgataat aagtgctatt gtaattatta tgcccatcgg
     3301 attcacgtaa attattattg atgtcttgca tccattggcc gtcgataatg aatttataaa
     3361 aatattttcc gggttttaat ttcacttcag caatccaacc tgagtcggtt ttttgcatgg
     3421 gcgtttggcc ggtactccaa tcattaaaat tgccacttaa aaatacttta cttgcttgcg
     3481 gaaaattaag tagtttaaaa acggttatat cttgatcgtt ctcgtatact gttggtgcac
     3541 ctttaaaatt attaacgcca taacttacag gtccgggata accgggtgta atactttcat
     3601 taacattcaa gttgaaaaaa ataggttgat tgccccattg cacttccata ttatcatcac
     3661 caactacttt cgtaataatt gctttgcgtt tagtaatttt tctgattttc caaccctctt
     3721 tgcctaactg accaagacta tgatgaataa ataaactgtc ttcatcaaga ccgaaataca
     3781 tcataagttt tttgtagtcg ggactgcgct gtttaatttt taccacaacc atattacctt
     3841 ctacacgaac cgcgttgccg gttatctggg cagacgaact gtcgggtatt cctattatta
     3901 aaaacaggca caggatacag tatgtaatta gtttattcat tccgttttaa tcgcagtata
     3961 aaaatacatg gttaatcgct tctttttatg attaatggtt acaattcctt tcgaaaggta
     4021 atcataacca agaattccgt taatattagt atcgtaaaca agctgtaaat agtttaggtt
     4081 agtaaggata aatggcatag cgacaaacga tttgcctcct attattactg aatccatttg
     4141 accgtttagg ataagttgtg agttgccatt tgaaccggtt aaacctcccg gggtggtaag
     4201 ttgaaagtgt ttaatgattt tattactcag tgtattactg attacattac gctcagctcc
     4261 ggtgtcgaga caaaatcgca actttttttc agcggcaact acatccataa aaataatgtc
     4321 gttggtgata tcgatggaca catctacatc gggtaattga gtgcgttcat ttcccacaat
     4381 cctgttacct tctttatcga gtttgtataa aatgagcaaa ttgttgcggt aatcgatttc
     4441 catttccact tctttaaaca aggatgcccc caataaacct aatattttga cacctcttgc
     4501 atcttctaaa tgacctaaat tcacaatatc tgcatccacg ttagtgtaaa acaaatctgt
     4561 gatgatgagt gagtcgattg tggtatttaa aatagtattt ccgccgcctg taataccgta
     4621 cgaaacgcct tcaccctctg cttttcggcc ttcagtaaaa taggttttgt ttaaaaccaa
     4681 atgaggagca ccagtatcta aaataaaatt cccaatgagt gaatcgaccc ttgcctcaac
     4741 gaggaataaa ttctgtaccc tttttaaagg gataaataac tcatcaaatt cgccttgcgg
     4801 aattgggtcg tcgaagcctt tatcggtatc cggaacgggt ggtacacctg ctaaggctat
     4861 actactgcca tagagcaata tacatgttat aagtgcaagg atataactac cgattttcaa
     4921 gcgaacaaat gtaattcatt ctgcctgtaa acctaatcat cttaacataa ttaaacattt
     4981 attgacggta aacggacatt ttttacctgt caaccatttt attggcccgg gatttaagga
     5041 atttttcagc cagaataact acaacagtcc ccaataaata cattacaatg tctaatgtat
     5101 taaacttact gcctataata accgttgcta ctttattgtc atctaatcct aatacttcta
     5161 caatgtggag atattgtaaa agttcgatga tacaagcaaa aacaaaaacg ccagcagcaa
     5221 caagtaactc gcgattgcta atccagattt ttacaaaggc ataaattaat attactacca
     5281 ataaatcacc aatatagggt cggataattt tgtcttttac aaacagggca attgctattt
     5341 caatgaataa aagacaaata gtaattaata aataccattt actttgttta tgcaaaccaa
     5401 atcctgaaaa caagtgcagc atatcaggca gatgaaggtt tatttcaata cgttgcttgt
     5461 atggttataa tgtaaatgca acgataaaaa tatccgccga agtgccgctg ttttttgagg
     5521 ataaccaacc cactacaagc agattacctt catagtctaa aacagcttcg tagatatcaa
     5581 aaaagtcgct ctcagttttg ccggcaggag taaatactgt ttttccatca tcaccaaaag
     5641 tttcatctaa cttaccattc ggtaaataac gggcaattac tatttcttct tcaatacttc
     5701 cacccttacc cacaacaaca aatttaccat cgggtaacat aacactgctt acggcttcct
     5761 gattaccttt ttttgcaaac cccgtttgtg tttttccggt aataccatat ttttttgaaa
     5821 catcaccgaa agcactgtaa tccaaaaatc ccattttatt gagtgccata cagccaaaat
     5881 cctgatcacc ggcatcggtt gtacccacta ggatataatt cccgttggcg tttaccataa
     5941 tatctgaaag ttcatctgaa gcttcatcaa atccgcctgt aaatgcttta ccatagttaa
     6001 atgtggtgtc ggctgaccca ttgcttaata ttcgttgaac ggtaaaattt tgaaatgcac
     6061 ttttggcatt cgacatatat tgcgtgcctc caatcaggta agtgccattg cttaacgcaa
     6121 ctacttttgt agctgtgcag gaattattaa taacattttt attgttgcta ttggtaatag
     6181 gaaatgaccc aacatacatc ccttcaaata aaaagttttc atcgataatt ccacttggtg
     6241 tgaattttat tatagcagtt tggtctaaca taatactatt gtttccacca actgcaatta
     6301 tgttattata aaaatcgagg tcaaaatcgt tgagtacata attactttta actgtgggat
     6361 atgcggtgcc gaatttactt tttgccagcc catttatacc gaaagtttta tcaggtgtac
     6421 catctgccaa taatcgcagc ataaaaaatt caggtgtttt atctttttca ttagctgtgc
     6481 cgccaattaa tattttgcca tcatattgca tcataatttt ggttatacta atttgatgtg
     6541 caaccggaat taatatttta ccatttttac caaaattagt ttgtagttgc cctttattat
     6601 tatattgagc aacaaaaacc tgtttgcctg catcatatcc tttttcggta gtgcctgcaa
     6661 ctaatacatt acccgattta tccatggtaa ttgctgtagc ataatcggca ctttgttgta
     6721 aatcggtaat aattacgcca ttgttaccaa aattcaacgc taattttcct gtttgagcat
     6781 agctgatttg cagctttaaa agtgcaatga aaagtaatga gtatcgcatt aatttgtgca
     6841 tttttccaaa ggtatttaat atggtgcttg aaagcaaaca atatgccatg atttcattgt
     6901 gaataatgac cgtaatacat ttcaaaaaaa ttgataaaaa tcaatcatat caggctgttt
     6961 aagtcatacc gacagcctta ataatgccta tatttttgaa ttgtaaaata ttcagttaac
     7021 ccattaaatg aaatgttatg acactggtta aaaaaggttg gacatcgcca ttcagcagca
     7081 actatttgtc agatttcttt aatactgaca agttatttgg cgataacttt ttcactaccg
     7141 aacggatgcc tgcgg
//
LOCUS       DAORKW010000136         7137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_332_length_7137_cov_4.956509, whole genome shotgun sequence.
ACCESSION   DAORKW010000136 DAORKW010000000
VERSION     DAORKW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_332_length_7137_cov_4.956509"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLB46_06950"
     CDS             <1..336
                     /locus_tag="PLB46_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRF76291.1"
                     /translation="TESVLVVLSSFVISIAVMFAFFGRFTNSKLFKTLSLQTSENNSE
                     GYNSTIFTEPASMKGKTGIAMSDLRPSGKIMIDGEWYDGQSDGDYILKGAEIKVVGVM
                     NAYLIVRLK"
     gene            353..1063
                     /gene="kdsB"
                     /locus_tag="PLB46_06955"
     CDS             353..1063
                     /gene="kdsB"
                     /locus_tag="PLB46_06955"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRF76292.1"
                     /translation="MKIIAVIPARFASTRFPGKPLVDIQGMSMIQRVYMQAKKVPEFS
                     SVIVATDDERIFHHVLAFGGEVVMTSANHISGTDRIGEVLNRISDIPDVVVNIQGDEP
                     FIQPEQLQLLLSAFHDHTTQIATLVKQISSPAEIENPNLVKVVFSNHHKALYFSRSII
                     PFARNVSTHYYKHIGIYAYREDVLHQLTQLSPTPLEQAESLEQLRWLENGFSITVVPT
                     EIETIGIDTPEDLNKILG"
     gene            complement(1338..1787)
                     /locus_tag="PLB46_06960"
     CDS             complement(1338..1787)
                     /locus_tag="PLB46_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF76293.1"
                     /translation="MEKFIVNTIEIQATPEVVWDALVNPEKTKIYMFGCATISDWQEA
                     SALLWQGSYEGKEMVFVKGFIIKIEAPYLLVYSVFDPNSTMEDVVSNYLHVTYKLAAN
                     GNSTLLTVMQGDYTTVADGERRYKESYNNGEGWNPILQAIKQLVEQA"
     gene            complement(1804..2409)
                     /locus_tag="PLB46_06965"
     CDS             complement(1804..2409)
                     /locus_tag="PLB46_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRF76294.1"
                     /translation="MDNKNIYKGIITTSAKSQNDWRKWLEKNHEKENAVWLIIAKKDS
                     GIASVNYPQAVDEALCFGWIDAVANKKDEDSFYQYFTKRKPKSKWSKINKEKVAQFIK
                     KGKMAPAGMAMVELAKATGTWDALNEVEELIIPDDMLKLFKKNKVAFENWKTFSRSIQ
                     RGILEWISNAKKEETRNKRIVETISLAEKNIKANQYQKKPD"
     gene            complement(2413..2961)
                     /locus_tag="PLB46_06970"
     CDS             complement(2413..2961)
                     /locus_tag="PLB46_06970"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HRF76295.1"
                     /translation="MLTPEQEKQISKLLSLVLRHQPEALGIVLDEQGWTDVKILIEKI
                     NNQGIQISFNELKLLVANNNKKRFALNETETLIRANQGHSIEVNLGYEPKQPPVILFH
                     GTGHLAVESIKKSGLDKRNRQHVHLSVDIETAIKVGQRHGKPVVFTVAAQLMFEKGYQ
                     FYVADNGVWLTEHVPSQFLTIE"
     gene            complement(2995..3558)
                     /locus_tag="PLB46_06975"
     CDS             complement(2995..3558)
                     /locus_tag="PLB46_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HRF76296.1"
                     /translation="MSTNKRQQQASTLRLFRKIHRFTGATLFVFFFIVAVTGTLLGWK
                     KHSNGVLLAETQSGTTSDLSKWLSLDTLQQLAIGYAHLHIDKNMSANLDRIDVRNEKG
                     IVKFVFAEGFWGIQVDGATGKLLSIERRRSDFIEKVHDGSILDEVFNTSNGQIKLIYT
                     SIMGLALLIFTITGFWLWYGPKRMRRQ"
     gene            complement(3575..4375)
                     /locus_tag="PLB46_06980"
     CDS             complement(3575..4375)
                     /locus_tag="PLB46_06980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF76297.1"
                     /translation="MDNQYLHINKHSWNNRVDAHFKSEFYDVQGFIQGKSSLNEIELQ
                     LLGDIKGKSILHLQCHFGQDTISLARLGAEVTGVDLSDKAIMQANVLAQQTHVAANFV
                     CCDIYDLPNHLNQQFDIVFTSYGTIGWLPDLNKWAAVIQQFLKPGGQFVFVEFHPVVW
                     MYDDDFSKVGYNYFNTGPIMETESGTYADRDAAIQQDYIMWNHGLAEVFTALLQNDLT
                     ISTFQEFDYSPYNCFKHTIEFAPGKFHIQHLGNNIPMVYALVANKKSI"
     gene            complement(4446..5471)
                     /locus_tag="PLB46_06985"
     CDS             complement(4446..5471)
                     /locus_tag="PLB46_06985"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF76298.1"
                     /translation="MLKNTTILIFFLALLVGQPLQAQLPTSVDSIYQVIKTNSVFTAS
                     ANWQVIDEMFEAKLVAASNDIDSIDALVEVFKQLDDVHSSITYNFKQYGHYHGMADSL
                     REIVLPVYEYGQSQINKLATAVLNGEYMYIRLPGYQVWGSDVHQYAQDIHDSICKYAT
                     YPVRGIVLDLRINTGGQISAMLGGLSLLLGEGKFGAAYAENGIIRYPFEIRENDFFMG
                     DEKRADITSNCTTGYHELPIAVLIGPVTSSSGTITAIAFKNRPLTQFIGEATADGYST
                     GNDYYVFGENLTMNLATTYFGDRTGVVYRNVVKPDVVISGKDEFLNLSADRKINYALM
                     WLKSIQH"
     gene            complement(5481..6203)
                     /locus_tag="PLB46_06990"
     CDS             complement(5481..6203)
                     /locus_tag="PLB46_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF76299.1"
                     /translation="MKLIAIVTDLHLGDDFPRANNVDAAQRWNIIFEDIKKRNVNQII
                     INGDIGDKPSNFVFFNELKYSGIPFTLTLGNHDFYDEAIKYFYRNNPNNDMELYYAEE
                     DEETLFIYLDSLTGTVSKNQCDWLATQLRSPKKIFIFIHHPILAVPTIVDTLYPLSNR
                     DEIANLLNQHTRPITICCGHYHIEHDQTVGNVRQLVTPAASVQIQQDDKEVIIDTTKF
                     GYRLLLLRNNVIQTDVIWFNNN"
     gene            complement(6906..>7137)
                     /locus_tag="PLB46_06995"
     CDS             complement(6906..>7137)
                     /locus_tag="PLB46_06995"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76300.1"
                     /translation="ESEIVEILTFPNPATNSIVISSKEDSLGNIYLYNASGMLLLNLK
                     VETEIVEIDVSKYASGQYWIRVGASVTGFMKE"
BASE COUNT         2208 a         1337 c         1164 g         2428 t
ORIGIN      
        1 accgaaagtg tattggtagt gctgagttca tttgtcattt cgatagccgt tatgtttgca
       61 ttttttggtc gtttcacaaa ttcgaaatta tttaaaactt tgtcgctgca aaccagtgaa
      121 aataacagcg aaggatataa ctccacaata tttaccgaac ccgcatcaat gaaaggcaaa
      181 acaggtattg ctatgagcga tttacggcct tcaggaaaaa taatgattga tggtgaatgg
      241 tacgacggac aaagcgatgg tgattatatt ctgaaaggcg ctgaaataaa agtagtaggc
      301 gttatgaatg cttatttaat tgtaaggtta aaataatatt tatttttaca acatgaaaat
      361 aattgcggta atacctgcac gatttgcatc aacacgattt cccggaaaac cattagtgga
      421 tattcagggc atgtcgatga ttcagcgtgt gtatatgcag gcgaaaaaag tacccgaatt
      481 ttcatccgta attgtagcta ccgatgatga acgtattttt catcatgtgc tggcatttgg
      541 tggcgaagtt gtgatgacaa gcgccaacca tatttccggt accgaccgta ttggtgaagt
      601 attaaacagg atatccgaca tacctgatgt agttgtaaat attcagggcg atgaaccttt
      661 tattcagccg gagcaattac aattattgtt atccgcattt catgaccata ccacgcaaat
      721 tgccacattg gtaaaacaaa tttcttcgcc ggcggaaatt gaaaatccca atctggttaa
      781 agtggtattt agcaatcacc acaaagcgtt atatttttcg agaagtataa ttccgtttgc
      841 tagaaatgtt tctacgcact actacaaaca tatcggcatt tatgcatatc gcgaagacgt
      901 tttacatcaa ctcactcaat tatctcccac accgcttgaa caagccgaat cgctcgaaca
      961 attacgatgg ctggaaaatg gttttagtat aaccgtagtg ccaactgaaa ttgaaacaat
     1021 tggtatcgat acacctgaag atttgaacaa aattttgggg taatctttgg ggcgaattaa
     1081 acacttgact tttaataatt actaaatgta taacccttat tttaatgggc tcaacggcat
     1141 ttatttgcat ttatgacatt taataatatc ggccattttg ctttgtagac gctgtatccc
     1201 accgtaatct tacatcatca aagctgcatc tgctagaact atgcaaacac aaacccacta
     1261 aagctcttgg gtgcagaaga gtacttgatt actcagggtt aaatcccgag ccggcaaatt
     1321 aatatttgcc ggttttttta tgcctgttct accagttgtt ttatggcttg gagaattgga
     1381 ttccaacctt ccccattatt gtatgattct ttataacgtc gttcaccatc agcaacagtt
     1441 gtgtaatcgc cttgcattac ggtgagtaaa gtagaattgc cgttagcggc taatttataa
     1501 gttacatgca aataattaga cactacatct tccatagttg aattaggatc aaataccgaa
     1561 tacactaata aataaggtgc ttcaatttta ataataaacc ctttcacgaa aaccatttcc
     1621 tttccttcat aacttccttg ccaaagtaaa gcgcttgcct cctgccaatc ggaaatggtt
     1681 gcgcagccaa acatgtatat tttagttttt tccggattta ccaaagcatc ccaaacaact
     1741 tcaggtgttg cctgtatttc gattgtattt acgataaatt tttccatgtg ttaaaagtac
     1801 aatttaatct ggcttttttt ggtattgatt agccttgata tttttttcgg ctagtgaaat
     1861 tgtttctaca attcgtttat ttcttgtttc ttctttttta gcattggaaa tccattccaa
     1921 aattcctctt tgaatggagc gcgaaaatgt tttccaattt tcaaatgcta ctttattttt
     1981 tttgaacaat ttcagcatat catcaggaat aatgagttct tccacttcgt tcaatgcatc
     2041 ccaggttcct gttgctttag ccaattccac catagccatt cctgcagggg ccattttccc
     2101 tttctttata aattgcgcta ccttttcttt atttatttta ctccatttac ttttcggttt
     2161 gcgtttggta aaatattgat agaaagaatc ctcatctttt ttattggcga cagcatctat
     2221 ccaaccaaaa cacaaggctt catctacagc ctgaggataa tttacagacg cgataccgct
     2281 atctttttta gcaataatga gccaaacagc attttccttt tcgtggttct tctctagcca
     2341 ttttcgccaa tcgttttgag atttagctga tgttgtaata attcctttat atatattttt
     2401 gttgtccatt gattattcta tagttaaaaa ttgtgacgga acatgttcag tgagccaaac
     2461 accattgtca gcaacataaa attgataccc tttttcaaac ataagctggg cggctactgt
     2521 aaacacgacc ggtttgccat ggcgttgccc aactttaatt gccgtttcaa tgtcaacact
     2581 caagtgcaca tgttgcctat tccttttatc taatccggat tttttaatac tttcaactgc
     2641 aagatgacct gtaccatgaa acaaaattac cggtggttgc ttcggttcat atcccaaatt
     2701 cacttcgata gaatgtcctt gatttgctct gattaatgtt tcagtttcgt tcaaggcgaa
     2761 ccgtttttta ttattatttg ctaccaataa tttcaattca ttaaaactaa tttgaatgcc
     2821 ttggttatta attttttcta ttaggatttt cacatctgtc cacccttgtt catccaaaac
     2881 tattcctaat gcttcaggct gatgtcgtaa aaccagactt agcagtttac tgatttgttt
     2941 ttcttgttca ggagtaagca cgttatcaat tttttaaagc aaaaacttat gttattattg
     3001 tctacgcatt cgtttaggac cataccataa ccaaaatccg gtgatggtaa aaatgagcaa
     3061 ggccagtccc ataatactgg tgtaaattaa tttaatttgc ccattagaag tattaaatac
     3121 ttcatctaat attgaaccat catgtacttt ttcaataaaa tcggaacgcc gcctttcgat
     3181 agataataat tttccggtag ccccgtcaac ctgaattccc caaaatcctt ctgcaaaaac
     3241 aaattttaca ataccctttt catttctaac atcaattcta tcgaggttgg cagacatatt
     3301 tttatctata tgcaaatggg catatccaat tgccagttgt tgcaatgtat cgagcgacag
     3361 ccatttactc aaatcgcttg tagtgccgga ttgtgtctct gctaaaagga caccattact
     3421 atgttttttc catcccaata aggttccggt tacagccaca atgaaaaaaa atacaaacaa
     3481 ggttgctccg gtaaaccgat gtatttttct aaataatcgt aaggtgcttg cctgttgttg
     3541 ccgtttattt gttgacatta aatttgataa cagtttagat tgatttttta ttggcaacta
     3601 acgcatatac cataggtata ttatttccta aatgttgaat gtgaaatttt cctggagcaa
     3661 attcaattgt gtgtttaaaa caattgtaag gagaataatc aaattcctga aatgtggata
     3721 tggttaaatc gttttgtaac aaagcggtaa atacttcagc caagccgtga ttccacatga
     3781 tataatcttg ttgaattgct gcatcgcgat cggcataagt gcctgattct gtctccataa
     3841 ttggccctgt attaaaataa ttataaccta ctttactaaa gtcatcatcg tacatccaaa
     3901 caacaggatg aaattctaca aatacaaatt gtccgccggg ttttaaaaat tgctgaatta
     3961 ctgcagccca tttatttaaa tcaggcaacc aacctattgt gccatatgaa gtaaatacaa
     4021 tatcaaattg ttgatttaaa tgattaggta aatcgtatat atcacaacaa acaaaattag
     4081 cggcaacatg ggtttgttgg gctaaaacat ttgcctgcat tatcgcttta tccgataaat
     4141 ctactccggt aacttccgcg cctaatcttg ccagtgaaat ggtgtcctga ccaaaatgac
     4201 attgtaaatg gagaatagac tttcctttta tatcacctaa cagttgtaat tcaatttcat
     4261 ttaaggaaga tttaccttga ataaatccct gcacatcgta aaattcagat ttaaaatgtg
     4321 catctaccct attattccat gaatgtttat taatatgtaa atattgatta tccatactaa
     4381 aagtaaagat aacaattaaa atacaatcgc atgaaaggca agggagacag gtatttcaaa
     4441 ggtgtttaat gttgaataga tttcagccac atcaatgcat aattaatttt tctgtcagca
     4501 ctcaggttca agaactcatc ttttcctgaa attactacat cgggttttac cacatttcta
     4561 taaactacac ctgttctgtc cccaaaatag gtagtagcaa ggttcatggt taaattttca
     4621 ccaaatacat aataatcatt tcctgtagaa taaccatcag ccgttgcttc accaataaat
     4681 tgagtcaatg gtcggttttt aaaagcaatt gccgtaattg ttcctgaact tgaagttacc
     4741 ggaccaatta ataccgctat tggtaattcg tgatatcctg ttgtgcaatt tgatgttata
     4801 tctgcacgtt tttcatcccc cataaaaaaa tcattttcgc gtatttcaaa cggatatcgt
     4861 attattccgt tttctgcata agcagcgccg aatttacctt ctcccaataa caaacttaat
     4921 ccgcctaaca tggcacttat ttgaccacct gtatttatac gcaagtccag cacaatgcct
     4981 ctgacaggat atgttgcata tttacaaata ctgtcgtgta tatcctgcgc atactgatgc
     5041 acatcacttc cccacacctg ataacctggc aaacggatat acatatattc gccattcaga
     5101 actgctgttg ctaatttgtt tatttgcgat tgtccgtatt cataaacagg taaaactatt
     5161 tcgcgcaacg aatcggccat accgtggtaa tgaccatatt gtttaaaatt gtatgtaata
     5221 gaagaatgta catcatccaa ctgtttaaat acttctacta aagcatcaat cgaatcgata
     5281 tcatttgatg ctgcaaccaa tttagcttca aacatttcgt ctatgacttg ccaatttgcc
     5341 gaagcagtaa aaactgaatt agttttaatt acctgataaa tagaatcaac tgatgttgga
     5401 agttgtgcct gcaaaggttg accgaccaac aaggcaagaa aaaaaatcag gatagtggta
     5461 ttttttagca ttttaaaaca ttaattatta ttaaaccaaa ttacatcggt ctgaattaca
     5521 ttgttacgca gcagtaataa gcgatagcca aatttagtgg tatcgatgat tacttcctta
     5581 tcatcttgtt gaatttgcac agaagcggcg ggagttacga gttgtcgcac attaccaacc
     5641 gtttgatcgt gttcgatatg ataatgccca caacaaatcg ttattggcct tgtatgctga
     5701 tttaataaat tagctatttc atccctattg gatagtggat ataaagtatc tacaattgtg
     5761 ggaacagcta aaatggggtg atgaataaaa ataaatattt tttttggcga acgaagttga
     5821 gtggctaacc aatcacattg atttttgcta actgtacctg ttagcgaatc caagtaaata
     5881 aatagtgttt cttcatcttc ttccgcataa tataattcca tatcgttatt tggattattc
     5941 cggtaaaaat attttatcgc ctcgtcgtaa aaatcgtgat tacctaatgt gagtgtaaaa
     6001 ggtataccgg aatattttaa ttcattaaaa aacacaaaat ttgaaggttt gtcgccaata
     6061 tcaccattaa ttataatctg attaacgttg cgttttttta tatcttcaaa aataatattc
     6121 cagcgttgtg cagcatcaac attatttgct ctggggaaat catcacctag gtggaggtcg
     6181 gttacaattg caataagttt caagttgaat tgtttgccac taagttagta aactccagga
     6241 tattatttta catatgcttc tatatcatca acatacaata ccatttcatt atcgaaagta
     6301 ttagaactta tctcaaggct aggtttgcgt tagaattttc aagtattagc tcgttagtca
     6361 aggcacttga agcagcctat attggagaaa gtacgggcaa ttcaagcagc gaagggaaaa
     6421 gagcggagac gaagaaaaga cgccaaaggt tttttttgag atgagttctt tacagattat
     6481 caggtattct ttagaggctt gattccttct aagttaattt ccatgcaact atttctgttc
     6541 aattttactc cagtattacc attggatttg atttatcaaa attactgtgt taaattgttg
     6601 atgttatcga tcaaagagtg aaaactgagg tggttttatt gattttcaac ataaatcatg
     6661 ttttaataac agcaagctaa atagtgcaaa ttctttatta tgagcattaa atatacttat
     6721 gaatttacat gcagtaatat ttatttaatt tatttaaaaa gctaggccaa tatttgaata
     6781 actatccacc atttcttgga ttatttaatt aaataaacaa acattttaat caatttattt
     6841 tgttgcttac ttacattaat ttatgttaat tattccttct atttacttgt taccttcatg
     6901 ctgaatcact ccttcataaa tccagtaact gatgctccaa ctctgatcca gtattgcccg
     6961 cttgcgtatt ttgatacatc tatttcaaca atttccgtct ccacttttaa atttaaaaga
     7021 agcataccac ttgcattata aagataaata tttcctaaag aatcctcttt tgaagaaata
     7081 acaattgaat ttgttgccgg gttcgggaaa gttagtattt ctactatctc agattcc
//
LOCUS       DAORKW010000137         7129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_333_length_7129_cov_4.449675, whole genome shotgun sequence.
ACCESSION   DAORKW010000137 DAORKW010000000
VERSION     DAORKW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_333_length_7129_cov_4.449675"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..638
                     /locus_tag="PLB46_07000"
     CDS             27..638
                     /locus_tag="PLB46_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRF76301.1"
                     /translation="MIHLNLHLNQKEFDKKVALITFGGAYTVLPYVYQGTVNYYHWLA
                     PTQMIDGLALGETTPGPLIMVLAFVGFMGGYQAFGSSLLAGSIGLLTTTYYTFLPFFF
                     FIFAGAPIIEKTQHNTHIKQILSFVTAAVTGVILNLTLYFAEAVIFGKTAENLLSINW
                     HEVQWLNVIWIVISVIALYRYKIDMLIWLAVSAIFGLTTYFIA"
     gene            674..3154
                     /locus_tag="PLB46_07005"
     CDS             674..3154
                     /locus_tag="PLB46_07005"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HRF76302.1"
                     /translation="MRYIKILACIVIPALVIYFLDTGIGSLPPLGKLMDPVNGFMANA
                     ETKTAFESNTVDITDPSVKGTIVMDDRLVPHVFADDERSLYYLQGYITAKYRLWQMDI
                     QTRAAAGRLSELFGPKFIEYDIEQRRKGMIFGAENALATIKNNPKLEACIQAYTDGVN
                     AFMQINETDGKSGLKYAAYPIEFKLLNYQPEAWTPLKTALLLKYMASTLTGFDDDAEM
                     TNLKKLLDEADFNALYPDYTTGIDPIIPTEKIYDFDAATTDTLASTTSDVYYKKVLDI
                     FEQQNQKNQVGSNNWAVGGSKTKNGYPILCNDPHLQLNLPSLWFEIQLQLGEMNCYGA
                     SLPGAPAIVIGFNDDIAWGVTNAQVDVRDWYTITYQDETKSAYKYGNGWRKTNKRIET
                     VNVRGAAAVIDTVIYTHYGPVVYENVKMVDGVKAKDKAAREGMAMRWLALEKSEEVNT
                     FYLLNRAKNYDDYRHAISYFSCPAQNFIFASHSGDIAITQQGKFPLKQKGQGKFILDG
                     ANPADDYQGYIPFEHNPTIKNPSRGFCASANQHPTDSTYPYYYTSFDFEFYRNRVINT
                     TLNKITGATVTDMQNLQQNNFNLAASEALPILLAKLADPCRTDENACKILTELKEWNY
                     ENNAVDLAPTYYQIWWDTLYNLMWDEMQSETIAYVKPNTYFTVEAMRNFHDNHPLFDF
                     KLTANKKETFADLVQQSFSSMISICDSLVKADKNVLQWYQHKHTNIMHISQIPAFSKL
                     MVKNGGYRNIVNATSETHGPSWRMIVELSSPVNAFGIYPGGQSGNPGSKYYDNFVDDW
                     AAGKYYPLYLFKDLDAAGKKALFTLTFN"
     gene            3157..3504
                     /locus_tag="PLB46_07010"
     CDS             3157..3504
                     /locus_tag="PLB46_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76303.1"
                     /translation="MKTAIIIIAGIIICYLLQTLLPIWWIFVLLTLLIGYVSKLASGW
                     KTFFVCWLIVFTTWIVLYILKDIANDSVMSSKMANLFSMKSNYWLFGIVSAVMGILGG
                     LTGVAGYYLSKKS"
     gene            3507..4406
                     /locus_tag="PLB46_07015"
     CDS             3507..4406
                     /locus_tag="PLB46_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76304.1"
                     /translation="MKTWDIVLLTEDRYCNPTELTPYIQNILTDDAILTKALEEKGLR
                     VIRKSWSDKFFDWSTTHYVVFRTTWDYFDRFPEFEIWLNATSEKTQFINSIQLLKWNL
                     DKHYFKDLLQAGVNTVETYYFKKGQTQKLSNLFAELKLNEAVLKPTISGSARHTYRLN
                     TSNVAKHNDIFSELIQHEDMMLQPFQYEIINKGEVAFMLMDGIFTHAVLKKSKPGDFR
                     VQDDFGGTLHDYTPTSGEIAFAEKAVTACSEKPLYARVDIINDNNGQLAVMELEIIEP
                     ELWFRRNPLSANVLANAIKNLFK"
     gene            4413..6356
                     /locus_tag="PLB46_07020"
     CDS             4413..6356
                     /locus_tag="PLB46_07020"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76305.1"
                     /translation="MKKLLLLICLPLLIISAKAQVASDYAVLVSSIVTEIPPTVELIW
                     PVYATATEYKIYKKEKSDPSWGTAVATLSGTAINYTDTDIIIDSAYEYKVERTASSGI
                     IATGYILCGIKMEVVDYRGTCLLVIDTTITMDMSEEIYQMKKDISGDGWSVEELHLAR
                     DMQPIAVREMIIARAEANEDITALYLFGRVPVLYSGNIYPDGHPEHQGAWPADAIYAD
                     VDVDYTDVTVDNIVAFRPENQNVPGDGKYDQSLLKSKVEMQIGRVDLFNMPTFVVDEA
                     TLLKRYLNSEHNFKHGIYTFNHRGLIDDNFGAFGGEAFASSGWRNFGPMFTANEIYEA
                     DYFTTLYNDSYLWSYGCGGGWYQGASGVGSTTEFAADTVKSAFTMLFGSYFGDWDAVD
                     NFLRAPLASGMTLTNCWAGRPHWQFHQMALGENIGYSVRVTQNNTNTYEANIFPHYVH
                     MGFMGDPTLRMHNFKPVAEVTVSQPDNTTIAYEIDFEPSPDIDVLGYHVYRSSTEFGK
                     YDRITTMLINEPYLDETPLPGNNFYMVKAVKLETTPSGSYYNTSIGITDSISNNLTAI
                     PQIEATQFELFPNPAFNVITITADASLTNAPYSIYNLEGKLCQTGSLYTNQSLIDIST
                     LTPGYYMIQINEEYAQFVKMVIE"
     gene            complement(6360..>7129)
                     /locus_tag="PLB46_07025"
     CDS             complement(6360..>7129)
                     /locus_tag="PLB46_07025"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HRF76306.1"
                     /translation="PFLLLHHHTGFIANDTHPREVGVSPHPHRGFSPVTFVFKGDIHH
                     RDSRGNSAVVKAGGVQWMDAGMGIVHSERPSVSFAKKGGQQEIVQLWINTPHAHKLDQ
                     PNYQALDEQTFPIISLPDEKGKLAVVAGKYGALQGPAKSKLDVIILRGDVLPGATYNL
                     QIPAKYNTLIYILNGSLTINENEQVAGLHLIQFNNDADNIHISTNQKTQFLVLAGLPL
                     NEPIESYGPFVMTNQTEIMQAIRDYQIGKMGILIEEW"
BASE COUNT         2336 a         1168 c         1354 g         2271 t
ORIGIN      
        1 aaatgatttc ctaaaaattg tagaaagtga tccacttaaa tttgcattta aaccaaaaag
       61 aatttgacaa gaaagtagca ttaataactt ttggtggggc ttatacagtt ctaccttatg
      121 tttatcaagg tactgtgaat tattatcatt ggttagcacc cactcaaatg attgatggat
      181 tagcacttgg ggaaacaaca cccggaccgc ttattatggt gttggcattt gtcggattca
      241 tgggaggtta tcaagctttc ggttcaagtt tgttggcggg ttcaattggc ttattgacta
      301 caacttatta tacttttttg cctttttttt ttttcatttt tgcgggagcg cctatcattg
      361 aaaaaacgca acataatacg catatcaaac aaatactcag ttttgttaca gcagcagtga
      421 caggtgtaat attaaattta accctgtatt ttgcagaagc tgtcatattt ggtaaaaccg
      481 ctgaaaattt gttgtctatt aattggcatg aagtgcagtg gttaaatgta atttggattg
      541 taatcagtgt gattgcactt tatcgttata aaatcgatat gttaatttgg ttggctgtaa
      601 gtgccatctt cgggttaaca acttatttta ttgcataata aactatcgtt atttttatgt
      661 ttaaatggct tatatgagat atatcaaaat acttgcctgc atagttatac ctgcacttgt
      721 tatttatttt ttagataccg gaataggctc gctgccacct ttaggtaaac tcatggatcc
      781 tgtgaacgga tttatggcta atgctgaaac aaaaacggca tttgaaagta atactgttga
      841 tattaccgat ccatcggtaa aaggaacaat cgtaatggat gatcgtttgg ttccgcatgt
      901 ttttgcggat gatgagcgat cattatatta tttgcaaggt tatattacag ctaaatatcg
      961 cctgtggcaa atggatatac aaacacgcgc tgctgccggt aggttaagtg aattgtttgg
     1021 tccaaaattt attgaatacg atatagaaca gcgtcgtaaa ggaatgattt ttggcgctga
     1081 aaatgcgctt gctacaatta aaaataatcc aaaattagaa gcttgtattc aagcatacac
     1141 cgatggtgtt aatgccttta tgcaaataaa tgaaactgat ggtaaaagtg gtttaaagta
     1201 tgctgcttat cccattgagt ttaaattatt gaattaccaa ccggaagcct ggacaccttt
     1261 aaaaacagcg ttgttattaa aatacatggc aagtacactt accggttttg atgatgatgc
     1321 tgaaatgact aacctaaaaa aattacttga tgaagcggac tttaatgcgc tttatccgga
     1381 ttatacaaca ggcatagatc cgattattcc aacagaaaaa atatacgatt ttgatgctgc
     1441 gacaactgat acattagcat caacaacaag tgatgtttat tataaaaaag tattggatat
     1501 ttttgaacag caaaatcaaa aaaatcaggt tggttcaaac aactgggcag ttggtggaag
     1561 taaaacaaaa aacggatatc ccattttatg taatgacccg catctacaat taaacttgcc
     1621 ttcgttgtgg tttgaaattc aattacaatt aggcgaaatg aattgttatg gtgcatcatt
     1681 accaggtgca cctgcaattg ttattggatt taatgatgat attgcatggg gagtaacgaa
     1741 tgcgcaagtt gatgttagag attggtatac catcacatat caggacgaaa cgaaatcggc
     1801 atataaatat ggaaatgggt ggagaaaaac caataaaaga attgaaacag taaatgtgcg
     1861 tggtgctgct gcagttattg atactgtaat ttatacccat tatggacctg ttgtgtatga
     1921 aaatgtaaaa atggttgatg gtgttaaagc aaaagacaaa gctgccaggg aaggtatggc
     1981 aatgcgctgg ttggctttag aaaaatcgga agaagtaaat accttttatt tactgaatag
     2041 agcaaaaaat tatgatgatt accgacacgc tatctcctac tttagttgcc ctgctcagaa
     2101 ttttattttc gcttcccatt cgggagatat tgctataaca caacaaggca aatttccatt
     2161 aaaacaaaaa ggtcagggaa aatttatttt ggatggtgct aatcctgcag acgattatca
     2221 gggatatatt ccatttgaac ataatccaac aattaaaaat ccgtctagag gtttttgtgc
     2281 atccgccaac cagcatccta cggattctac ttatccatat tattatacca gtttcgattt
     2341 cgaattttat cgcaaccgtg taattaatac taccttaaat aaaataacag gtgcaacagt
     2401 aacagatatg caaaatttac agcaaaataa ttttaacctt gctgcgtcag aagcattacc
     2461 aattttattg gcaaaattag ctgacccatg tagaactgat gaaaatgcct gtaaaattct
     2521 tactgaactt aaagaatgga attacgaaaa taatgcagtt gatttagctc caacctatta
     2581 tcaaatttgg tgggatacgt tgtataactt aatgtgggat gaaatgcaaa gtgaaactat
     2641 tgcttatgta aaaccgaata cttactttac tgtggaagcg atgcgtaatt tccatgacaa
     2701 tcatccgcta tttgatttta aattaactgc taacaaaaag gaaacatttg ctgacttggt
     2761 tcaacaaagt tttagcagca tgatatccat ttgtgattca ttagttaaag cagataaaaa
     2821 tgttttgcaa tggtatcaac acaaacatac taatatcatg catatcagtc agataccggc
     2881 atttagcaaa ctcatggtaa aaaatggcgg ttatcgcaat attgtgaatg caaccagcga
     2941 aacccacgga ccaagttggc gcatgatagt tgaactttca tcacctgtaa atgcatttgg
     3001 aatttatccc ggtggccaat cgggtaatcc tggtagtaaa tactacgata attttgttga
     3061 cgattgggct gcaggaaagt attatccttt gtatttattt aaagatttgg atgcggcagg
     3121 taaaaaagca ttattcacat taacatttaa ttaattatga aaacagcaat aataattata
     3181 gcaggaatta taatctgtta tttacttcaa actttattac ctatttggtg gatatttgtt
     3241 ttacttaccc tattgatagg ttatgtttcg aaactagctt ccggctggaa aacatttttt
     3301 gtttgctggt taatcgtatt tacaacatgg attgtattat atatcctaaa agatattgct
     3361 aacgacagtg tgatgagcag taaaatggcc aacttatttt ctatgaaaag taactactgg
     3421 ttatttggga tagtatctgc agtaatggga attttaggtg gtctgactgg tgtggccggt
     3481 tattacttgt cgaaaaaatc gtgagcatga aaacctggga tattgtttta ttaactgaag
     3541 accgttattg caatccgaca gaactcactc catatatcca aaatatttta accgatgatg
     3601 ctattttaac caaagcattg gaagaaaaag gattaagggt tattagaaaa tcgtggagcg
     3661 ataaattttt tgactggtcg accacacatt atgtggtatt tcgaacaaca tgggattatt
     3721 ttgacaggtt tcccgaattt gaaatatggt taaatgcaac ttccgaaaaa acacaattta
     3781 ttaattccat tcagttatta aaatggaatt tggataaaca ctatttcaaa gatttattac
     3841 aagccggggt taatacggtt gaaacatatt atttcaaaaa aggccaaact caaaaactca
     3901 gtaatttatt tgcggaactt aaactcaacg aagcagtgct taaacctact atatcaggct
     3961 ctgcacgtca cacctatcgt ttgaacacat ctaatgttgc aaaacacaat gacatcttta
     4021 gtgagctcat tcaacatgaa gatatgatgt tgcaaccatt tcagtacgaa ataattaaca
     4081 aaggtgaagt ggcttttatg ttaatggatg gtatctttac acatgccgta ttaaagaaat
     4141 cgaagccggg agattttaga gtacaagatg attttggcgg tactttgcat gactacacac
     4201 ccacttcagg cgaaatagca tttgctgaaa aagctgtgac tgcttgttct gagaaaccat
     4261 tatatgctag ggttgatatt attaacgaca acaatggcca acttgcagta atggaattag
     4321 aaataattga accggaattg tggtttagaa gaaatccgtt atctgccaat gttttagcga
     4381 atgcaattaa gaatttgttt aaataacctt gtatgaaaaa actattactc ctgatttgtt
     4441 tgccattatt aattatttct gcaaaagcac aagtggcttc agactacgct gttttagttt
     4501 catcaatagt tactgaaata ccacctactg tagaattaat ttggccggtt tatgcaacag
     4561 ccacggagta taaaatttat aaaaaagaaa aatcagaccc ttcttgggga acagctgttg
     4621 caacattaag tggaaccgca attaattata ccgatacaga tattattatt gattctgcat
     4681 atgaatataa agttgaacgc acagccagtt ccggaattat tgctaccgga tatatattat
     4741 gtggaataaa aatggaagtg gtagattata gaggaacatg tttattggta attgatacta
     4801 ccattacaat ggacatgtct gaagaaatat atcaaatgaa aaaagacatt tccggtgatg
     4861 gctggagtgt tgaggaattg catctagctc gcgatatgca accaattgca gtaagggaaa
     4921 tgattatagc acgggctgaa gcgaatgagg atattactgc attgtattta ttcggaagag
     4981 ttccggtttt atattccggc aatatatatc ccgacgggca tccggagcat cagggagcct
     5041 ggccggctga tgcaatttat gcagatgttg atgtagatta tactgatgta acagtagata
     5101 atatagtagc attcagaccc gaaaatcaaa atgttccagg tgatggaaaa tatgatcagt
     5161 cgctgttaaa atcaaaagta gaaatgcaaa taggaagggt tgatttattt aatatgccaa
     5221 cttttgtagt ggatgaagca accttactaa aacgatattt aaacagtgag cataatttca
     5281 aacatggtat ttatacattt aatcatcgcg gattaattga tgataatttt ggcgcatttg
     5341 gtggcgaagc atttgcgtcg agtggctgga gaaattttgg accaatgttt acggcaaatg
     5401 aaatttatga ggccgattat tttacaaccc tatacaacga tagttattta tggagttacg
     5461 gttgtggtgg cggatggtat cagggagcaa gcggtgttgg ttctacaact gaatttgccg
     5521 ctgatactgt aaaatctgct tttacgatgt tattcggtag ttattttggg gattgggatg
     5581 ctgttgataa ttttttacgt gcaccattag cttcaggtat gacattaaca aattgctggg
     5641 caggtcgacc acactggcaa tttcatcaaa tggcactagg tgaaaatata ggatatagtg
     5701 ttcgggtaac gcaaaataat acaaatacat acgaagccaa tattttcccg cattatgtgc
     5761 atatgggttt tatgggtgat ccaactttac gtatgcataa tttcaaacct gttgctgagg
     5821 taactgtcag ccaaccggat aatactacca ttgcttacga aattgatttc gaaccttcac
     5881 cggatatcga tgtgttaggt tatcatgttt atcgctcatc aacagaattt ggtaaatatg
     5941 atagaattac aactatgttg attaacgaac cttatttgga tgaaacacca cttccgggca
     6001 ataattttta tatggtaaaa gcagttaaac tagaaacaac accgagtggt tcatattaca
     6061 ataccagtat tggtattact gattcaatca gcaataattt aacagccatt ccacaaatcg
     6121 aagctacaca atttgaatta tttccaaacc ctgcttttaa tgtaataacc attacagctg
     6181 atgcatcatt aacaaatgca ccttatagta tttataattt ggaaggaaaa ttatgtcaaa
     6241 ctggttcatt gtataccaat caatccttaa ttgatatcag cacattaact ccgggatatt
     6301 atatgattca aattaatgag gaatatgccc aatttgtaaa aatggttatt gaatgagttt
     6361 taccactctt caattagtat ccccattttc ccaatctgat aatcgcgaat agcctgcata
     6421 atttcagttt gatttgtcat cacaaaagga ccataacttt caataggttc gtttaatggc
     6481 aatcctgcta aaaccaaaaa ttgtgttttt tgattggtgc ttatatgtat attatctgca
     6541 tcgttattaa actgtattaa atgcaaacct gctacctgtt cgttttcgtt aattgtcaaa
     6601 ctgccattca aaatataaat aagggtatta tattttgcag gtatctgtaa attataagtt
     6661 gcaccgggaa gtacatctcc ccttaaaata attacatcta attttgattt agcagggcct
     6721 tgcaatgctc cgtattttcc agctacaact gcaagttttc ctttttcatc cggcaacgaa
     6781 ataattggaa acgtttgctc atctaaagcc tgataattgg gttgatccaa tttatgtgca
     6841 tgtggcgtat ttatccataa ttgcacaatt tcctgttgcc ctcctttttt ggcaaatgaa
     6901 acggatggtc tttcactatg cacaataccc ataccagcat ccatccattg cactccacct
     6961 gctttaacta ctgctgaatt tcctcttgaa tcgcgatgat gaatatctcc tttaaacaca
     7021 aatgttaccg gcgaaaatcc acgatgcgga tgcggcgaca ctcccacttc tcgaggatga
     7081 gtatcattag caataaatcc ggtatgatga tgcaaaagta aaaacgggt
//
LOCUS       DAORKW010000138         7104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_334_length_7104_cov_5.000709, whole genome shotgun sequence.
ACCESSION   DAORKW010000138 DAORKW010000000
VERSION     DAORKW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_334_length_7104_cov_5.000709"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /gene="gpmI"
                     /locus_tag="PLB46_07030"
     CDS             complement(<1..859)
                     /gene="gpmI"
                     /locus_tag="PLB46_07030"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRF76307.1"
                     /translation="MNSGKKAMLIIMDGWGHGKNPAASAIAQAKTPNVNSYYNQYPNA
                     ELRTDGEFVGLPEGQMGNSEVGHLNLGAGRIVYQELQRIFVAIKTGELANNPNLLAAF
                     NYAKTNNKNIHLIGLVSDGGVHSHINHLKALCDIAKNNQVGGDKLFIHAFTDGRDCDP
                     KSGAGFLKDLQQHLNNSIGTIASVCGRYYAMDRDNRWERIKLAYDLLVKGTGKKTTDI
                     IAAVEASYAENVTDEFILPIVHTNAAGNPLAVIQPDDVVICFNFRTDRCRQITRVLTQ
                     EDFPDLGMFT"
     gene            998..1399
                     /locus_tag="PLB46_07035"
     CDS             998..1399
                     /locus_tag="PLB46_07035"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HRF76308.1"
                     /translation="MTKNSKTRLFITMLIVPIMIFATKAGDLENIAAAISSGNSKELA
                     TYFDNTVEVKIANKEGAYSKSQAEAIIKDFFTKNPPTSFSFIHEGNSGGNNAHYAIGN
                     LVTSKGTYRTYVYLKKINNKFVIQELKFDNE"
     gene            1392..2252
                     /gene="nadC"
                     /locus_tag="PLB46_07040"
     CDS             1392..2252
                     /gene="nadC"
                     /locus_tag="PLB46_07040"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRF76309.1"
                     /translation="MSDSVVIWDLLDIIDEALIEDIQEGDHTTLATIDHEAAGTAKLL
                     VKEPGIIAGVELAEQILKHVNPELEVDIFIPDGARVEPGDIVLTASGRVQALLVAERT
                     MLNFMQRMSGIATNTNRFVQAVRHTEAKILDTRKTTPGLRTFEKWAVRIGGGHNHRFG
                     LYDMIMIKDNHVDFCGGIENAINRVHQYLADNELNLRIEVETRNLDEVWQVLKTGRVD
                     RIMLDNYRLEDLREAVKVIGKQFETEASGGVNLNTVKAIAETGVDFISVGELTHSVKS
                     LDLSFKAVVA"
     gene            2351..4276
                     /locus_tag="PLB46_07045"
     CDS             2351..4276
                     /locus_tag="PLB46_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRF76310.1"
                     /translation="MKEQLFHIEILLKRLGIVLILYSACRLLFFVFNMDLFPPIPPGE
                     FLQIMGYGLRYDVSSIIYVNIIFIILHVIPNPYFDTPRYQRFLKILFYSFNGLALLLE
                     SGDWIYFEYGLKRTDAHELGLTGDTNILPQVIKDYWIIFVIAFLLILGVEYFYRKTEI
                     HRKVPKVPSPRLIHYPKQLIIMLVIVTFGVIGARGGIQQEPLSPNEAANVVEDGRYSS
                     LIINTPFSVIYAFGHRELHEPKYFEPEELSKRFQIFHENVKYYQRPDTIPAPKQPENV
                     CVIILESFSKEYIGYFNPGQPYTPFLDSLMGVGLTFTNAYSNGKSSNQGIIAVTTGIP
                     VLMGEPFISSIYQHNDFEGIGTILDRVGYSSYFFHGANNGSMEFDRFTEEAGFTGYFG
                     RNEYGNDKDFDGNWGIFDEPFLKWTANKMSTLPAPFYSEIFTISSHHPFTVPKQHIGK
                     FPKGQIPMLEVVAYGDYALRKFFEEAKKQPWYENTLFILTADHPGPPLPEHEFYQEQV
                     GAHSTWMVVYKANGEFKGTSEMIVQQTDIMPTVLDYVGYRGKFMAFGNSIFDTIAERY
                     AYNFHTPDYMILDNNYFLQFNAGRAIGLYEYKKDSTMSSNLLFEYPDVTASMEQKLKA
                     IIQTHHHVMINNKLVAE"
     gene            4273..5160
                     /locus_tag="PLB46_07050"
     CDS             4273..5160
                     /locus_tag="PLB46_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRF76311.1"
                     /translation="MKKRILFIINPVAGVKRKDKIPALINAHLDHDRFDYEVVYTEKR
                     GHATEIAAEAAKNNFDIVAVAGGDGSVNEVATGLMGTSTSLAIIPSGSGNGLARHLGY
                     SIDIKSTLAIINAYEVRKMDVCKINDEYFFSLIGIGFDAFVAKVFSREETRGFMTYAW
                     SAIKSLGSFESFDYTLQSKEKNITGKAFMINVCNSNQYGYNVKVAPDANLSDGLMDVI
                     IVDDIPKWKVPFAVMQVFTQTHKVNRHFKSFKTSALQINSPNYSYLQIDGETVHKDKD
                     FTITILPQQLNVLINPNLK"
     gene            5162..5503
                     /locus_tag="PLB46_07055"
     CDS             5162..5503
                     /locus_tag="PLB46_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HRF76312.1"
                     /translation="MAKNKINLSGLVFSTDPNFKMPEDVQHDTTLPPQQQDLRIWLEK
                     NHRGGKTASVIKNFVGSTTDLENLCRELKTKCGTGGSAKDGEIIIQGDHRKKLGEILS
                     KMGYKYKLAGA"
     gene            complement(5541..5876)
                     /locus_tag="PLB46_07060"
     CDS             complement(5541..5876)
                     /locus_tag="PLB46_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRF76313.1"
                     /translation="MQTIQYSDFEKIEIRTGTIVGVSDFPNAKKPAYQLFIDFGELGI
                     KKSSAQITSHYSKQALIGKQIAAVVNFPVKQIANFFSECLVLGAIQIDGSIILLQTEQ
                     EVQNGLKIA"
     gene            complement(5994..>7104)
                     /locus_tag="PLB46_07065"
     CDS             complement(5994..>7104)
                     /locus_tag="PLB46_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF76314.1"
                     /translation="TISVAGRQNDIWAATQNNGVQHLETTTGNLTIYNQLNTEFVTND
                     FRSIAVSPTTIYAGTFNNGFYTFYNNEWTHFDTINSPLPGMQVNDFCLYNDSIIFIAT
                     DNGLVKIENEIWTIYDSTLIAANKLTCLYKDNSNRLWIGSKYHGVTKLEDGIFTNYNY
                     ENAGINNDWIRAISSNSSGLMFIADYFGVNTYQPETDDWLFVYNTFTAPMSSERVNKI
                     GFQQNGTLWFATHIGVTSADTSNFWTQYYDYNSNLPHNVTDGLYIDDEDRVWLATYGG
                     VAVFTSTDNLPTTSTDLQVYPNPCSQQLTISANPGSLISVYDIQGQSVANLIKIDHNF
                     GEQQIQIDVSALPKGVFLVCMEIDTSISTKVFVKQ"
BASE COUNT         2199 a         1292 c         1352 g         2261 t
ORIGIN      
        1 atgtaaacat acccaaatcc ggaaagtctt cctgcgttaa tacacgcgta atttgtcggc
       61 agcggtcagt tctgaaatta aaacaaatta ctacatcatc aggctgaatt actgccaacg
      121 gattgccggc tgcattggta tgtacaatcg gtaaaataaa ttcatcagta acattttctg
      181 cataactagc ttctacagct gcaataatat cagttgtttt tttacctgtt ccttttacca
      241 ataaatcgta agccagttta attctttccc aacggttatc tctgtccatc gcgtaatatc
      301 gtccgcacac gctggcaata gtgccaatac tattatttaa atgttgttgt aaatctttta
      361 aaaatcccgc gccacttttt gggtcacaat cacgaccatc agtaaatgca tgaataaata
      421 atttatctcc gcctacctga ttgtttttgg caatatcgca taatgctttt aaatgattaa
      481 tatgtgaatg cacaccacca tcacttacca aacctattaa gtgaatattt ttattatttg
      541 tttttgcata attaaaagca gccaataaat tcggattatt cgccagttca cctgttttaa
      601 tggcgacaaa aatgcgttgt aattcctgat aaacaattct gccggcgccg aggttgagat
      661 gtcccacttc actgttgccc atttgtcctt ccggtaaacc gacaaattcg ccgtcggttc
      721 gtaattctgc attgggatat tggttgtaat aactatttac attaggcgtt tttgcctgtg
      781 caatggcact agctgccgga ttttttccat gtccccaccc atccattatg atgagcatcg
      841 cttttttacc actattcatg ttacaaagtt agcagagata tcgcattttt aggctggtat
      901 attgttatca acgatggtaa acactagttt tgttaaaaat tcctaatttt ggtatggttt
      961 tagccaatac cttagcattg accaaattag tacaaccatg acaaagaatt caaaaaccag
     1021 attgtttatc acaatgctta ttgtgccgat tatgattttc gccacaaaag cgggtgattt
     1081 agaaaacatt gccgcagcca tcagcagtgg taactctaaa gagttggcaa cctatttcga
     1141 caatacggtt gaagtaaaaa tcgctaacaa agaaggcgct tatagtaaaa gtcaggctga
     1201 agccattatt aaagactttt ttacgaaaaa ccctccaact tcgttcagct ttatccacga
     1261 aggtaattcg ggtggaaaca atgcacatta tgccattggt aatttagtta ccagtaaagg
     1321 cacttataga acttatgtgt atttgaaaaa aatcaacaac aaattcgtta tccaggagtt
     1381 aaaattcgat aatgagtgac agcgtagtta tatgggattt actcgatata attgacgaag
     1441 cccttattga agatatacag gaaggtgacc acaccacact tgctactatt gaccatgagg
     1501 ctgccggaac ggcaaaattg ttggtgaaag aacctggcat tattgccggt gtagaactcg
     1561 cagaacaaat tcttaaacat gtcaaccccg aattggaggt tgacattttt attcccgatg
     1621 gcgccagagt ggagccggga gatatcgttt taaccgcatc tggcagggtg caagccttgt
     1681 tagttgccga acgcaccatg ctcaacttta tgcagcgtat gagcggtatt gctaccaata
     1741 ccaatcgctt cgtacaagct gttagacata cggaagctaa aatactggac acccgtaaaa
     1801 ctacgccggg actgcgcacc ttcgaaaaat gggcggtgcg tataggcggt ggacataacc
     1861 ataggtttgg gttatacgat atgattatga ttaaggataa tcacgtagat ttttgcggag
     1921 ggattgaaaa tgccattaac cgcgtgcacc aatatttggc tgacaacgaa ctgaatctgc
     1981 gtattgaagt ggaaacccgc aatctggatg aggtttggca ggtgctcaaa acaggccgtg
     2041 tcgaccgtat tatgctcgat aattatcgtt tggaagacct tcgggaggct gttaaagtaa
     2101 ttggaaaaca gttcgaaaca gaagcctcag gtggtgttaa cctgaacacc gtaaaagcaa
     2161 tagcagaaac aggtgtagac ttcatttctg taggtgagct gacgcattcg gtgaaaagtc
     2221 tcgacctcag tttcaaggct gttgtagcct aagtttagtt gttaaagtag cttaattgta
     2281 tttccggccg ccgaatactt gtaaataacg gcttatttat ttattacctt tgtggtctga
     2341 aaaccaacat gtgaaagagc aattatttca tattgaaatt ttattaaaac gattaggtat
     2401 agtattaatt ctatattctg cttgccgttt gctttttttt gtgtttaaca tggatttatt
     2461 tccacccatt cctcccggcg aatttttgca aattatggga tatggtttgc gctacgatgt
     2521 atcatctatt atttacgtga acatcatttt tatcattctc cacgttattc ctaatccgta
     2581 tttcgataca cctcgttatc agcgcttttt aaaaatatta ttttattcct ttaatggttt
     2641 agccttattg ttagaaagtg gtgactggat ttattttgaa tatggattaa aacgcaccga
     2701 tgcacatgaa ctcggtctca ccggcgatac caatatttta cctcaggtaa ttaaagatta
     2761 ttggatcatt tttgtgattg cctttttgct gatattagga gttgaatatt tttaccgcaa
     2821 aactgaaata catcgcaaag taccaaaagt tccatcaccc agactaattc attatccaaa
     2881 acaattaatc attatgttgg tgattgttac gtttggtgta attggtgcaa gaggtggtat
     2941 acaacaagaa cctttatcgc cgaatgaagc ggcaaatgtt gtggaagatg gcagatatag
     3001 ttcacttatt atcaatacgc cgtttagtgt gatttatgca tttggtcatc gcgagttaca
     3061 cgaaccaaaa tattttgaac cagaagaatt atctaaacgt tttcagattt ttcatgagaa
     3121 cgtaaaatat tatcaacgtc ccgataccat tcctgctcca aaacaaccgg aaaatgtttg
     3181 tgtaattatt ctggaaagct tttcgaaaga atatatcggt tactttaatc cgggtcagcc
     3241 ttatacgcca tttttagatt cactgatggg tgtcgggtta acatttacca atgcgtattc
     3301 aaatggcaaa tcatctaacc aaggtattat tgctgttact accggtattc ccgttttaat
     3361 gggcgaacct tttatttctt ctatttatca gcacaacgat tttgaaggta taggaaccat
     3421 tctcgacaga gttggttatt cctcgtattt ctttcatggt gccaataatg gctcaatgga
     3481 gtttgacaga tttactgaag aggctggatt taccggttat ttcggaagaa atgaatatgg
     3541 taacgataaa gattttgatg gtaattgggg catttttgat gagccatttt taaaatggac
     3601 cgctaataaa atgagcacat tgcctgcacc attttatagt gaaattttta ctatttcttc
     3661 tcaccatcca tttactgttc ctaaacaaca tattggtaaa tttccaaagg gacaaatccc
     3721 aatgttagag gttgttgctt atggcgatta tgctttacgc aagttttttg aggaagctaa
     3781 aaaacaacca tggtatgaaa ataccttatt cattttaaca gccgatcatc ccggtccacc
     3841 gctgcctgaa catgaatttt atcaggaaca ggttggtgcg cattcaacat ggatggtggt
     3901 gtataaagct aatggagagt ttaaaggcac ttctgaaatg attgtgcagc aaactgatat
     3961 tatgcctacg gttttagatt atgttggcta ccgcggtaaa tttatggctt tcggtaattc
     4021 tatttttgat actattgccg aacgttatgc ctataatttt catacgccgg attacatgat
     4081 attggataac aattatttcc ttcaatttaa tgctggacgt gccattggat tgtatgaata
     4141 taaaaaggac agtaccatga gctcgaattt gttattcgaa tatcctgatg ttacagcttc
     4201 catggagcaa aagttaaaag ctattattca aacacatcat catgttatga taaataataa
     4261 attagttgca gagtgaaaaa acgcatctta tttattatca accccgtagc cggtgtaaag
     4321 cgaaaagata aaattcctgc gctgataaat gcgcatttgg accacgaccg gtttgattat
     4381 gaagtggtgt acactgaaaa acgcggacac gcaacagaaa tagctgctga agcagcaaaa
     4441 aataatttcg atatagttgc agttgccggc ggcgatggca gtgtgaatga agtggcaacc
     4501 ggtttaatgg gcacttctac cagtttagct ataattccca gcggctcagg aaacggactg
     4561 gcgaggcatc taggttattc tattgatatt aaatctacac tggcaattat caatgcctat
     4621 gaagtccgca aaatggacgt ttgtaaaatt aatgatgaat attttttcag cctgatagga
     4681 attggatttg atgcatttgt ggctaaagtt tttagtcgcg aagaaacacg aggatttatg
     4741 acctatgcct ggagtgccat caaaagttta ggttctttcg aatcgtttga ttatacttta
     4801 caatcgaaag aaaaaaatat caccggaaaa gcatttatga taaacgtatg taattccaat
     4861 caatatggtt acaacgtaaa ggtggcaccc gatgcgaatt taagcgacgg attaatggat
     4921 gtgattattg tagatgacat ccctaagtgg aaagtacctt ttgctgttat gcaggtattt
     4981 acacaaacac ataaagtgaa ccgacatttt aaatcgttta agacaagtgc tttacaaatt
     5041 aattcaccca attattctta tttacaaatt gacggtgaaa cagtgcacaa agacaaagat
     5101 tttaccatta ctattttacc tcagcaatta aacgttttaa ttaaccctaa cctgaaataa
     5161 aatggcaaaa aataaaatta atttatccgg cttagtgttt tctaccgacc cgaattttaa
     5221 aatgcctgag gatgtgcaac acgataccac tttacctcct caacaacagg atttgcgtat
     5281 ttggttagaa aaaaatcacc gcggtggtaa aacagccagc gtgataaaaa actttgttgg
     5341 cagcacaaca gatttagaaa acctttgccg cgaactcaaa acaaaatgcg gaaccggtgg
     5401 tagtgcaaaa gatggcgaaa ttattattca gggtgaccat cgaaaaaaac tcggtgaaat
     5461 tttatccaaa atgggatata aatataaact cgcaggagct tgatgcagct tcctaatgca
     5521 aattaaaggt tcaatattat ttaagcgatt ttcaaaccgt tttgaacttc ctgttcggtt
     5581 tgtaatagta tgatactgcc gtcaatctgt atggcaccca gtaccaaaca ttctgaaaaa
     5641 aagttggcta tttgttttac cgggaaattc actacggcgg caatttgttt tcctattaag
     5701 gcttgttttg aataatgcga agtaatttgt gcactcgatt tttttatgcc caattcacca
     5761 aaatcaataa ataattgata cgccggtttt ttggcatttg gaaaatcact tacaccaaca
     5821 atggttccgg ttcgaatttc aattttttca aaatcgctgt attgaattgt ttgcattgta
     5881 atagttttga attaagtaaa gttaatgcaa attagttggc tgtttataat taataggcaa
     5941 atatagtaac ctgtcacatg ctgtaaatac aatgagtaaa taaaatacac gggttattgt
     6001 tttacaaata ccttggtgct tatacttgta tctatttcca tacaaactaa aaacacgcct
     6061 tttggcaaag ctgagacatc tatttggatt tgctgctcac caaagttgtg gtctattttt
     6121 attagattgg caactgattg gccttgaatg tcataaactg aaattaaact ccctgggtta
     6181 gcacttatag taagttgttg actgcaggga ttgggataaa cttgtaaatc tgttgatgtg
     6241 gtaggtaaat tatcagtaga agtaaaaact gcaaccccgc cataagttgc taaccaaact
     6301 ctgtcttcat catcaatgta taatccatcg gttacattgt gtggtaaatt ggaattgtaa
     6361 tcataatact gtgtccagaa attgctggta tcggcactgg ttacaccaat atgagttgca
     6421 aaccataaag tgccattttg ttgaaacccg attttattta cgcgttctga actcataggt
     6481 gcagtaaatg tattatatac aaacaaccaa tcgtccgttt cgggttgata ggtatttaca
     6541 ccaaaataat cagcgataaa cattaatccg ctactattac ttgaaatggc gcgtatccaa
     6601 tcattgttta ttccggcatt ttcataatta taattagtaa aaatgccatc ttccaatttg
     6661 gtaacaccat ggtattttga accaatccaa agtcggttcg agttatcttt atataaacag
     6721 gtaagtttgt ttgctgcaat taaagtggaa tcataaatag tccaaatttc attttcaatt
     6781 tttactaaac cattatctgt tgctataaaa atgatactat cattatacaa acaaaaatca
     6841 tttacctgca taccgggcaa tggagaattt atggtgtcga aatgtgtcca ttcattatta
     6901 taaaacgtat aaaagccatt attaaacgtt ccggcataaa tagtagttgg tgacactgca
     6961 atacttctga aatcgttggt aacaaattcg gtattcaatt ggttgtagat ggttaaatta
     7021 ccggtagtgg tttccagatg ttgcacacca ttgttttgtg ttgccgccca aatatcattt
     7081 tgccggccgg caacactgat ggtt
//
LOCUS       DAORKW010000139         7082 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_336_length_7082_cov_5.744557, whole genome shotgun sequence.
ACCESSION   DAORKW010000139 DAORKW010000000
VERSION     DAORKW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_336_length_7082_cov_5.744557"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PLB46_07070"
     CDS             <1..673
                     /locus_tag="PLB46_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF76315.1"
                     /translation="QDSPDEIPELYKMIAVDGLDLVSGWKKRRHDPISKTIPSKFFNW
                     TTSQITGIKLHDFNCGLKAYKKDLVKSIEVYGEMHRYIPQIAKTAGFYKIGEKVVEHR
                     ARKYGQSKFGWERFMKGFLDLTSIMFVSKFGKKPMHLFGTMGFLSFFIGGVITIWLII
                     EKLLKMNSGEKYRQVTDQPLFYIALVAIIVGVQLFVAGFLGELISRNSPDRNTYQVDQ
                     QIGIS"
     gene            670..1788
                     /locus_tag="PLB46_07075"
     CDS             670..1788
                     /locus_tag="PLB46_07075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRF76316.1"
                     /translation="MKIVLLGTVWPYRGGLASFNERLLKEFQDQGHEVLAITFTVQYP
                     GFLFPGKTQYSTSPAPEYLSIQRKVNSINPLSWFKVGRELKKMAPDIIIIKYWMPFMA
                     PAFGTIAKIAKKNKVTKVVCIADNIIPHEPKFYDKSFTRYFVNQCDRFVVMSASVLKD
                     LLQFNRTKLRATNPHPLFDNFGTKLPKADACKYLKLDVSKNYILFFGFIRDYKGLDLM
                     LQAMALIKSDVELIIAGEYYTDEKPYQQLITSNQLQSRVHQFNHFIADEEVKYFFSVA
                     DMVVQPYKHATQSGVTQIAYHFEVPMVVTNVGGLPEMVPHNHVGYVTEPSATDIANAI
                     DLFYTENRAAEFTSNLKEEKKRFGWDRMVETILALVAR"
     gene            complement(1785..3110)
                     /locus_tag="PLB46_07080"
     CDS             complement(1785..3110)
                     /locus_tag="PLB46_07080"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRF76317.1"
                     /translation="MSIQPKLKQYDLTMIVVSLVIGVGIFRTPAVVAEKAGSEWIFYA
                     AWIFGGLVSICGALTFAEIGSRLPAAGGYYKIFSYAYHPAYAFMLNWSQVIINAGSSA
                     GVAIIGAEYITPVLFSNSTQTAVIEKIIAIGIILFLFAINFAGIKMGARTQNFLSILK
                     IVMITAFCFAIFGKHAPESESILNTSNDINFISAFGVALISIFFTYGGYQQTVNFGAD
                     VENARTNIPKAIFAGMGIVICLYLLLNFTYVQVIGFETMKTSPLIASTLAEIIFGPIG
                     ATIISIVLFTSVLGFLNTSVMSNPRVYYAMAEDKILPPVFLSVNPKTQVQQFGLTFFI
                     AVMLLSVFLLGDFENIVNYVLFIDSISLASAAAAIFILRKKSNANYDGFKMKGYPILP
                     FVFMATLLFVTYNTLVADPKSSLYGFIIFLGGLPLYFLMHRMFNKSKTH"
     gene            complement(3135..3905)
                     /locus_tag="PLB46_07085"
     CDS             complement(3135..3905)
                     /locus_tag="PLB46_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76318.1"
                     /translation="MRYIFILACITNIFCSSCDVQTDDSRDTDPIIQLTTLEATPNAP
                     DFDKNIYDRVAVVNDSGEFYIDGRILDKADTAYLIGTKVGRSNLTEWVNADDYKKYHS
                     SFSAIQGIIIDAYKIRIGEGRLSKIVWLSGKNIYPAPWINIDTLKQGDSVIIQRFGDF
                     EPYKGVLTQDVKEHTDYVIIKYNEYNGSDAFNFDEVFIQKYPAQLADIRPGAIMYFDK
                     MYWVMILGTRDNTHIIVRQSGFPAKDMIVPISKLEVYK"
     gene            complement(4012..5313)
                     /gene="icd"
                     /locus_tag="PLB46_07090"
     CDS             complement(4012..5313)
                     /gene="icd"
                     /locus_tag="PLB46_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRF76319.1"
                     /translation="MSGQKITIANGKLNVPDNPIIPFIEGDGTGADIWAASVRVFDAA
                     VEKAYGGKRKIEWKEVLAGEKSFNATGSWLPDETLDAFREYLVGIKGPLTTPVGGGIR
                     SLNVALRQILDLYVCLRPVRYFEGVPSPVKTPEKVDMVIFRENTEDIYAGIDWQGNGE
                     LATKFLNWLKENSPKDFDKIRFGSKEKADAWQKDLTSVGMPFRDIDTMVGIGIKPVSY
                     LGTERLVYAAIDYAIKHNRKSVNIVHKGNIMKFTEGGFRDWGYQVAKEFFGAVEIDGG
                     PWCVIPEGKPGAGIVIKDSIADITLQQVLTRPDEFDVIATLNLNGDYLSDALAAQVGG
                     IGIAPGANINYVSGHAIFEATHGTAPKYANQDKVNPGSVILSGCMMFEYLGWQEANDL
                     IIKGIEKSIAAKRVTYDFHRLMEGATMLKCSEFGDEIISHM"
     gene            5462..6898
                     /locus_tag="PLB46_07095"
     CDS             5462..6898
                     /locus_tag="PLB46_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRF76320.1"
                     /translation="MNKQPELKRALGLTTAILLVISSMIGSGVFKKIAPMSTTLMDSN
                     LILLAWLLAGIISMFGVFTYSGLASLTEEGGGQFEYFRIIYGRFFGFLFGWTCFTVIQ
                     SASIASIAYVFSESVNNLAPFGSPFESWANVHIGNFIFPFANSGVKLFAIATIIALTI
                     INYFGVKKGGVINDIFSWGKIAGILFLIFAGFFLYHTKSDITTQNAQLIQAQDFNLIS
                     GLFTAMLAAFWAYDGWVNLSYIGGEVKNPKRNIPIAIIGGTAVVMIIYTLVNVVYLRV
                     LPVQTFVDIDAANNKIAAAEVAQTILPGIGYTLISVLIMLSTFGATNASLMSSPRIYH
                     QMAKQKLFFKSFGETHPKYHTPHKSLFYQMIWSSVLVMSGTFDQLTDMLIFASFIAYG
                     SGAAGLLYMKMRGKKVINEVDGSVQRVKITAKVIGYPVIPIILLLVAIGLVINTLITM
                     PNESLTGLSFIAIGIPIYYIVKKQNTNS"
BASE COUNT         2280 a         1317 c         1242 g         2243 t
ORIGIN      
        1 gcaggacagc cccgatgaaa ttcctgaatt gtataaaatg attgctgttg atggtttaga
       61 tttagttagt ggctggaaga aaagacgaca cgaccctatt tctaaaacga taccttctaa
      121 attttttaac tggactacat cgcaaattac cggaataaaa ctgcacgatt ttaattgcgg
      181 attaaaagca tataaaaaag atttagtaaa aagtattgag gtgtatggtg aaatgcatcg
      241 ttacattcct caaatagcta aaactgccgg attttataaa ataggcgaaa aagttgtcga
      301 acaccgtgcc cgtaaatatg gtcaatccaa atttggatgg gagcgattta tgaagggatt
      361 tttagattta acctccatca tgtttgtttc caagtttggc aaaaaaccaa tgcacctttt
      421 tggaaccatg ggatttttat ctttttttat aggaggtgtc atcaccattt ggctgataat
      481 agaaaaatta cttaaaatga attccggtga aaaatatcgc caagtaactg accagccttt
      541 gttttatatt gcgttagtag caattattgt tggggtgcaa ttatttgttg ccggattttt
      601 aggagaatta atttcccgga attcaccgga tagaaatacc taccaggtag atcaacagat
      661 tgggatatca tgaaaatagt tttgctcgga acagtatggc catacagggg cggtttagca
      721 tccttcaacg agcgtttgtt aaaagaattt caagaccagg ggcacgaagt attggcaatt
      781 acatttactg ttcaataccc cggtttttta tttcccggaa aaacacaata ttcaacttct
      841 cctgcaccgg aatatttatc tattcaacgc aaagtaaatt caataaaccc gttaagttgg
      901 tttaaggttg gccgcgaatt aaaaaaaatg gcccctgata ttattattat taaatattgg
      961 atgcctttta tggcgcctgc attcgggaca attgcaaaaa ttgcaaaaaa gaataaggtc
     1021 actaaagttg tgtgtattgc agataatatt atcccgcacg aacctaagtt ttacgataaa
     1081 tcatttacac gttattttgt taatcagtgc gacaggtttg tagtaatgag tgcgagcgtg
     1141 cttaaagatt tattgcagtt caacagaact aaattacgtg caacaaatcc tcatcctttg
     1201 tttgacaatt tcggaacaaa attacccaaa gctgatgcat gcaaatattt gaaactggat
     1261 gtttcgaaaa attatatatt gttttttgga tttattcgcg actataaagg gctcgattta
     1321 atgttacagg caatggcatt gataaaaagt gatgtagaac taattatagc cggtgaatat
     1381 tatacagatg aaaaacctta tcagcaactc ataacttcaa atcaactcca gtcccgtgtc
     1441 caccagttca atcattttat tgcagatgag gaagtgaaat acttttttag tgtggctgac
     1501 atggtggtgc aaccctataa acatgctaca caaagtggtg taacacaaat tgcttatcat
     1561 tttgaagtgc caatggtagt tactaatgtt ggaggtttac ccgaaatggt accgcataat
     1621 catgttggtt atgttactga gccttcagct actgatattg ctaatgctat cgatttattt
     1681 tacactgaaa atcgtgcagc agaatttacc tccaatctta aagaagaaaa aaaacgtttt
     1741 ggttgggata gaatggtgga aaccatcttg gcattagttg cccgttaatg tgtttttgat
     1801 ttattaaaca ttcggtgcat cagaaaatac aaaggtaacc cgcctaaaaa tataataaac
     1861 ccatataagg aagatttcgg atcagccact aaagtgttgt aagttacaaa taggagggtt
     1921 gccataaaca caaacggtag gataggatac cccttcattt tgaaaccatc gtaattagca
     1981 tttgattttt ttcttaaaat aaaaatggca gcggcggcgc tagcaagact aatactgtcg
     2041 ataaatagaa catagttgac aatattttca aaatcgccaa gcaaaaaaac agacaataac
     2101 attacagcaa taaaaaaagt taacccaaat tgttgtacct gtgtttttgg atttacggat
     2161 aaaaataccg gaggtaaaat tttatcttct gccatagcat aatatacccg tggattgctc
     2221 attaccgaag tatttaaaaa acccaatact gaggtgaata atacaatgga aataatagta
     2281 gcacctattg gtccgaaaat aatttctgct aatgtgctgg caattaatgg tgaagttttc
     2341 atggtttcga accctattac ctgcacataa gtaaaattga gtaacaggta taaacaaata
     2401 acaataccca ttcctgcaaa tatggctttt ggaatatttg tcctggcatt ttcaacgtcg
     2461 gccccaaaat ttacggtttg ctgatatcca ccataggtga aaaatataga aattaatgcg
     2521 acgccaaatg cactaatgaa attaatatcg ttgctggtat ttaatattga ttcactttcc
     2581 ggcgcatgtt taccgaaaat ggcaaaacag aaagccgtta tcatcacaat ttttaaaatg
     2641 gataaaaagt tttgtgtgcg ggcacccatt tttatgccgg caaaattgat agcaaataaa
     2701 aataaaatta ttcctatagc aataattttt tcaatgacag ctgtttgcgt tgaattggaa
     2761 aataacacag gcgtaatata ctccgcacca attatggcaa ctccagcact actccctgcg
     2821 ttaataataa cctgactcca gtttaacata aatgcataag caggatgata ggcataagaa
     2881 aatattttat aataaccgcc cgcagccggt aagcgtgaac caatttctgc aaaagttaaa
     2941 gcaccacaaa tagataccaa tcctccaaat atccaggccg cataaaatat ccattcgctt
     3001 cccgcttttt cggcaacaac tgcaggagtt ctaaaaatac ctacacctat taccaggctc
     3061 accactatca tggtaaggtc gtattgtttg agtttgggtt gaatggacat agatgaattt
     3121 ataactgtaa tttattattt atacacttct aatttactta tcggcactat catatctttt
     3181 gcaggaaatc cactttgtcg aacaataata tgggtattgt cacgggtacc taaaatcatt
     3241 acccaataca ttttatcaaa atacatgatg gctccgggac gaatatcggc aagttgagcc
     3301 ggatattttt gaataaatac ttcatcaaaa ttgaaggcat cgctaccatt gtattcattg
     3361 tatttgataa ttacataatc agtatgctct ttaacatctt gtgtgagcac acccttataa
     3421 ggttcaaagt cgccaaatcg ctgaataatc accgaatcac cttgtttgag tgtgtcgata
     3481 ttaatccaag gtgcagggta aatatttttt cctgaaagcc aaacaatttt gctcaatctg
     3541 ccttctccaa tacgaatttt ataagcatct attataatac cttgaatagc actaaatgag
     3601 ctgtgatatt ttttataatc atccgcattc acccattctg ttaaattcga cctaccaact
     3661 tttgtgccta ttaaataagc ggtatcagct ttatctaaaa ttctaccgtc gatataaaat
     3721 tcaccactat cattcactac agcaaccctg tcgtaaatat ttttatcaaa gtccggagca
     3781 ttgggagttg cttccaatgt ggttaattga ataataggat cggtgtcccg ggaatcatcg
     3841 gtttgtacat cgcaggaact gcaaaaaata ttagttatac atgccagaat aaaaatgtat
     3901 ctcataccag tgtgtaaaca aatataccat ttacttacaa aatcatgggc acaaaaaaag
     3961 gagcctcaac gagactcctt ttttgtattt agctttttgt aattggtgat attacatatg
     4021 actgataatt tcatcaccaa actcactaca ttttaacatg gtagcgcctt ccattaatcg
     4081 gtggaagtcg taagtaacac gtttagcagc aattgatttt tctatccctt taataataag
     4141 gtcatttgct tcctgccatc ctaaatattc aaacatcata caaccactta aaataactga
     4201 acccggattt actttatcct gattagcata tttaggcgct gtaccgtgag ttgcttcaaa
     4261 tattgcatgt ccgcttacat aattaatgtt agcacccgga gcaataccga taccgccaac
     4321 ctgagcagct aacgcatcgc ttaaataatc gccgttgagg tttaatgttg caataacatc
     4381 aaattcatca ggacgagtta atacttgttg taaagtgata tcagcaatag aatctttaat
     4441 tacgatacct gcacctggtt ttccttcagg aataacacac catggaccgc cgtcaatttc
     4501 aacagcacca aaaaattctt tagcaacctg ataaccccag tctctgaaac caccttcagt
     4561 gaatttcatg atattacctt tgtgtacaat atttacactt ttgcgattat gtttgatagc
     4621 ataatcaata gcagcataaa ctaaacgttc agttcctaaa taacttaccg gtttaatacc
     4681 aataccaacc atggtatcaa tatcgcggaa tggcatacca acgctggtta aatctttttg
     4741 ccatgcatct gctttttctt tagagccaaa acggatttta tcaaaatctt ttggagaatt
     4801 ttctttcagc cagtttaaaa atttagtagc taactcgcca tttccctgcc aatcgatacc
     4861 tgcatatata tcttcggtat tttcgcggaa aattaccatg tctacctttt caggcgtttt
     4921 aaccggagat ggaacacctt cgaaataacg cacagggcgc agacaaacat ataaatctaa
     4981 aatttgacgt aaagccacat ttaatgagcg aataccgcca ccaaccggag ttgttaacgg
     5041 acctttgata ccaactaagt attcacggaa agcatctaac gtttcatccg gtagccagct
     5101 acctgttgca ttaaatgact tttcacctgc taaaacctct ttccactcaa ttttgcgttt
     5161 tccgccatac gctttttcaa ccgctgcatc aaaaacgcga accgaagccg cccaaatatc
     5221 agcacctgtg ccatcgcctt ctataaatgg tataatagga ttatcgggaa cattcagttt
     5281 cccgtttgca attgtaattt tttgaccact cattgttata cttttttaat ttgaccgcaa
     5341 agatacgatt caggtggcag tagtcaatga attttcataa tcatccttac accgggcgct
     5401 ttaacaaatc gtaaacaggt tgaaaacgga tactgttata acttttcctt attcttacat
     5461 catgaacaaa caaccggaac taaaaagggc actcggctta actacggcaa ttttgttggt
     5521 tatcagctct atgattggta gtggggtatt caaaaaaatt gctcctatga gtactaccct
     5581 tatggatagc aatctcattt tattggcttg gttgctggcc ggtataatat ctatgtttgg
     5641 tgtgttcacc tattcaggcc tggcatcatt aaccgaagaa ggaggtgggc agtttgaata
     5701 tttccgaatt atttatggca ggttcttcgg ctttttattt ggatggacct gttttacggt
     5761 aattcaatcg gcatcaattg catccattgc gtatgtattt tccgaatcgg taaataatct
     5821 ggctccattc ggttctcctt ttgaatcatg ggcgaatgtc catatcggaa attttatttt
     5881 cccatttgcc aactcgggag ttaaattatt tgccattgct accattattg cattaacaat
     5941 aattaattat ttcggcgtaa aaaaaggtgg ggtaattaac gatatttttt cgtggggaaa
     6001 aattgcaggt attctttttc tcatatttgc cggattcttt ttatatcata caaaaagtga
     6061 tataaccaca caaaatgcgc aattaattca agcgcaggat tttaatttaa tttcagggtt
     6121 atttactgcc atgttagccg cattttgggc ttatgatggt tgggtgaatt tatcgtacat
     6181 aggaggtgaa gtaaaaaatc ctaagcggaa tattccaatt gccataattg ggggaacagc
     6241 tgtggtaatg attatttata ctttagtaaa tgttgtgtat ttacgcgtgt tacctgttca
     6301 aacttttgtg gacatagatg cagcaaataa taaaattgca gcagcagaag ttgcacaaac
     6361 tattttaccc ggaataggat atactttaat atctgtttta attatgttga gcacatttgg
     6421 cgctactaat gccagtttaa tgtcatcgcc acgaatttat catcaaatgg caaaacaaaa
     6481 attatttttt aaaagttttg gtgaaacaca tcctaaatat cataccccac ataaatcatt
     6541 attttatcaa atgatttgga gcagtgtgtt agtgatgtcg ggcacttttg accaactgac
     6601 cgatatgctc atttttgctt catttattgc ttatggcagt ggtgcagctg gtttattata
     6661 tatgaaaatg cgcggaaaaa aagtaatcaa tgaagtagat ggttctgttc agcgggtaaa
     6721 aattactgcc aaagtaattg gttatcctgt tattccaata atattgttac ttgtggcaat
     6781 tggactggta attaatacgc tcattaccat gcctaatgaa agtttaaccg gattgagttt
     6841 tattgctata ggtattccta tttattatat cgtaaaaaaa caaaatacca attcataaat
     6901 tttattgaat tagaattggg ataaaataag aaaggagccc caagcggagc tcctattctt
     6961 tagataatac ttctcttaac agccattcag aagtattaat ctgtcagcac aaccagaaat
     7021 ttagaggatt tccagaacgc atccggatta tcaataacta attcctcaat tgttttgtca
     7081 cc
//
LOCUS       DAORKW010000140         7080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_337_length_7080_cov_5.633452, whole genome shotgun sequence.
ACCESSION   DAORKW010000140 DAORKW010000000
VERSION     DAORKW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_337_length_7080_cov_5.633452"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1417
                     /gene="atpB"
                     /locus_tag="PLB46_07100"
     CDS             245..1417
                     /gene="atpB"
                     /locus_tag="PLB46_07100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRF76321.1"
                     /translation="MKSSNKVLSTVILGLVMLMAGQVSAASEGKYDAKEFAMHHVADA
                     HEWHIVGGLTIPLPCIVIHDGLHVFLSNKMPSAHHGEATEATEEAHAAVTDTIHAQGA
                     EGTMIVEHHETGEHTAHHETYNGFYYSESGRIVHEDGGFTLDLSITKNVATLLIVSII
                     MLLVFTSVAKAYKRREGEAPKGLQSFMEPLILFVRDEVVRPNLGKKSDKYLPYLLTVF
                     FFIWISNMMGLIPIIANPNLSGNIAVTCALALMTFLITNLHANKYYWGHIFNTPGVPG
                     FVKVILVPIEIAGIFIKPVALMIRLFANITAGHIIIISLVGLIFVFGNAGENLGGGLG
                     GAAVAVPFALFMSVLELLVAFLQAFIFTMLSALFISLAQEDHHHDDHAEHASAAHH"
     gene            1450..1692
                     /gene="atpE"
                     /locus_tag="PLB46_07105"
     CDS             1450..1692
                     /gene="atpE"
                     /locus_tag="PLB46_07105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRF76322.1"
                     /translation="MDLLTIMQAAAEPVKGIAAIGAGLAAIGAGIGVGRIGGSALESM
                     ARQPEVLGEIRTNMIIAAALVEGVALFAVVVALMQG"
     gene            1789..2310
                     /gene="atpF"
                     /locus_tag="PLB46_07110"
     CDS             1789..2310
                     /gene="atpF"
                     /locus_tag="PLB46_07110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRF76323.1"
                     /translation="MTTMTLLGAFDIITPDPGLFIWTVLVFLILLWLLNKFAFGPIKD
                     ALKKREEGIADALHQAQKAREEMANMKADNEKILNEAREERSRMLREAKDAKDQIITE
                     AREKANAEYNRIVDEAKGAINNQKMAALIEIKNNVGNMVLEVSEKILRRELDNKADQE
                     QYIKQLVDEIKVK"
     gene            2351..2911
                     /gene="atpH"
                     /locus_tag="PLB46_07115"
     CDS             2351..2911
                     /gene="atpH"
                     /locus_tag="PLB46_07115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRF76324.1"
                     /translation="MSSTKLATRYAKSVLDFAKEKGKLSEVLADMRLLNSSIYNNREF
                     YMMLKSPIVNGDKKMTVVLNVFHDKIDEITENFIGILMRKNRESHLPEIIDAFIAQYN
                     AFMHITPVTITTAHPISDDVQNTLLAKLKSTVGLEQIELTTEIDENLIGGYILHWDDK
                     MIDNSISRGLAILKDEFDNNDYVRKF"
     gene            2972..4552
                     /gene="atpA"
                     /locus_tag="PLB46_07120"
     CDS             2972..4552
                     /gene="atpA"
                     /locus_tag="PLB46_07120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRF76325.1"
                     /translation="MVDVKPDEISAILRQQLSNFKTSSDLEEVGTVLQVGDGIARIYG
                     LNNAKSGELVTFENGVRAIVLNLEEDNVGVVLMGSSDEIKEGDTVRRTGNIASIKVGE
                     SMLGRVVNALGEPIDGKGPLSGELYEMPLERKAPGVIYRQPVNEPLQTGLKPIDAMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQREFYEAGKPVFCIYVACGQKNSTVAQVAKT
                     LTDHGAMDYTIIVAASASDPAPLQFYAPFSGAAIGEFFRDTGRPALIVYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKVINNDEIASQMNDLPASLVGKVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETNLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQYRELEAFSKFGSDLDAATMAVLNKGQRNVEILKQKQY
                     APMSVEKQIAIIYLGTKGMLGKIPVDKIKAFEDAYLTYLETHHKATLDGLKAGQLTDE
                     ITGTLEKVAADMSKQYSA"
     gene            4597..5484
                     /gene="atpG"
                     /locus_tag="PLB46_07125"
     CDS             4597..5484
                     /gene="atpG"
                     /locus_tag="PLB46_07125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRF76326.1"
                     /translation="MAGKLKEVRTRITSVISTQQITKAMKMVSAAKLRRAQDRILQMR
                     PYALKMGELLSNIADAAGQDVKIDFAQSREVKSVLLVAITSDKGLCGAFNANVMKQIK
                     KTIEDNYSHLPKANVKIMFIGKKGYDVFKRSAYTLNTDFMNLFTAVDFENVSRATAYI
                     KEGFLKGEFDEVQVVYNKFKNAATQILETEQFLPIKKLEPVAGAKNVKSDYIYDPEKE
                     QMILELVPKILNTSFYRYILDSNASEHGARMTAMDKASDNANELLKELKISYNRARQA
                     AITTELTEIVSGAAALQGN"
     gene            5725..6204
                     /locus_tag="PLB46_07130"
     CDS             5725..6204
                     /locus_tag="PLB46_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76327.1"
                     /translation="MKIAYKRLIFVFLGTCIFFINEVHAADSLTVSNDLNEIAQFEMQ
                     LHNLELNLAETRALNNGRFDNFNLWLTILSGILLVIGMFTGYRTMAIARTEAREAFDE
                     KFKIYCDLITNKSEEAEALVLQMRSHHETSLAITEDLNTAIDKILKKSKKDEEGNKK"
     gene            6182..6532
                     /locus_tag="PLB46_07135"
     CDS             6182..6532
                     /locus_tag="PLB46_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76328.1"
                     /translation="MKKETKSEVILEFIKDLKELIQKIENNEETMQDYILLQEMFELS
                     NVPDDYISDCYLGSGFQNWNELYHSRRKNSRQAPNNKHLESAISKIRGISSAIISVMQ
                     KYVAENSNLYNKDH"
     gene            complement(6561..7010)
                     /locus_tag="PLB46_07140"
     CDS             complement(6561..7010)
                     /locus_tag="PLB46_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76329.1"
                     /translation="MGIKPKFKLKAIVYFRIIFHSVLPKNAGISFVYLSVYEKNKKWD
                     AYIQAAKSGAEKYLWNDANSLRRISDMIYDHSSDKSTLVKAANFAVRSAELKPEYFNN
                     LSAAKIYFKLGYNDLSKKYAQQAIAEGKKKNMNTVEAENIVTTISNN"
BASE COUNT         2296 a         1197 c         1433 g         2154 t
ORIGIN      
        1 cacataattg attgtgtaaa aaccgaaatg ccgcccagta ccagcagcat agccgtaaaa
       61 agtaattttg ctttcatttt ctttagagtt tgagctaaca atttaagctt tttcgggcat
      121 tttgacacat ttcttccttg aaaaaaaact ttttctcatt cctgtcaagg ttaacccctt
      181 tcttttttct ttttttagta ttgaagcctt acctttgcgg cgaaattcaa aatttatcac
      241 tatcgtgaag tcgtcaaaca aggttttatc tacggttatt ctgggcttgg taatgctaat
      301 ggcagggcag gtttcagcag cctcagaggg gaaatatgat gccaaggaat tcgctatgca
      361 ccacgtagct gatgcacatg aatggcatat tgtgggtggt ttgaccattc cgctgccttg
      421 tattgtgatt cacgacggtc tgcatgtgtt tttaagcaat aaaatgcctt cagcgcatca
      481 tggtgaagca acagaggcca cagaagaggc gcacgcagcc gttacagata ccattcacgc
      541 tcaaggtgca gaaggtacta tgatagtgga acaccacgaa actggcgaac atactgctca
      601 tcacgaaaca tataacggat tttattatag cgaaagtggc cgtattgtgc acgaagacgg
      661 tggttttacc ctcgatcttt ccatcaccaa aaacgtggct accttattaa tagtgtcaat
      721 tattatgtta ttggtgttta catcggtagc aaaagcttac aaacgccgtg aaggtgaagc
      781 accaaaaggc ttacagagtt ttatggagcc ccttatctta tttgtacgcg atgaggttgt
      841 aagacctaat cttggcaaaa agtcggataa atatttacct tatttactta ctgtattctt
      901 ttttatctgg atttcgaata tgatgggatt gattccaatc attgccaatc caaacctttc
      961 aggaaatatt gcggttacct gcgctttggc gttgatgacc ttcctgatta ccaatttaca
     1021 cgctaataaa tattactggg gacatatttt taacacaccg ggagtgcctg gttttgtgaa
     1081 agtgatttta gtgccgattg aaatagcagg tatatttatt aagccggttg cgcttatgat
     1141 acgtttattt gcgaacatca cagcgggcca tattatcatt atcagcttgg ttggtctcat
     1201 tttcgtattc ggaaatgccg gtgaaaacct gggtggtggt ttaggtggag cagcagttgc
     1261 tgtgccattt gcattgttta tgagtgtgct tgaattgctg gtagcatttt tacaggcttt
     1321 cattttcact atgttaagtg cattatttat ttcattagca caggaagacc atcaccatga
     1381 tgatcatgcc gaacatgcta gtgcagcgca tcattgatta ttattattaa caattataaa
     1441 aacagtaaca tggatttatt aactattatg caggcagcag ctgaaccagt taaaggtatt
     1501 gcagcaattg gtgccggttt agcagctatt ggagctggta tcggtgtagg ccgtatcggt
     1561 ggtagcgctc ttgagtcaat ggctcgtcaa ccagaagttc tcggcgaaat cagaactaac
     1621 atgattatcg ctgcagcact tgttgaaggg gttgcactct tcgcagtagt tgttgccctt
     1681 atgcaagggt aaaaagaaat aaaatcagtg gcaacggttg gttgctgctg atttttctta
     1741 gtgaaattaa aaaggttatt cttggaataa cccatcaaaa aatagaatat gacaacaatg
     1801 acacttttag gtgctttcga cattattaca ccggatccgg gtttatttat ctggacagtt
     1861 ttggtgtttc ttatcttatt atggttgtta aacaaatttg ctttcggtcc cattaaagat
     1921 gcattgaaaa aacgtgaaga aggaattgcc gatgcattac atcaggctca aaaagcacgt
     1981 gaagaaatgg ccaacatgaa agccgacaac gaaaaaattc ttaacgaagc ccgtgaagaa
     2041 agaagcagaa tgttgcgcga agctaaagat gctaaagacc agattattac tgaagcacgc
     2101 gaaaaagcaa atgctgaata caatcgtatt gttgatgaag ctaaaggagc tattaataac
     2161 caaaaaatgg ccgctttaat tgaaatcaaa aacaatgtgg gaaatatggt gcttgaagtt
     2221 tctgaaaaaa tattgagaag agaattagat aataaagctg atcaggaaca atacatcaaa
     2281 caattggttg acgaaattaa agtgaaataa atattgtcag ctttacgcta acgacaaaat
     2341 accattatca atgtcatcaa caaaattagc aacacgttac gcaaaatcgg tactggactt
     2401 tgccaaagaa aaaggcaaat tatcagaagt gctggctgat atgcgtttgt taaattcatc
     2461 tatatacaac aatcgcgaat tttacatgat gctgaaaagc cctattgtaa atggcgataa
     2521 aaaaatgaca gttgtattaa atgtgtttca cgataaaatc gatgaaatca ccgaaaactt
     2581 tatcggtatt ttaatgcgta aaaatcgcga atcacatttg cccgaaatta ttgatgcatt
     2641 tattgcgcag tataatgcgt tcatgcacat tacaccggtt acaattacaa ctgcacatcc
     2701 aatttctgat gatgttcaaa atacattgtt ggcgaaatta aaatcaacag ttggattaga
     2761 gcaaattgaa ttaacaaccg aaattgatga aaatctaatt ggtggttata ttttacactg
     2821 ggacgataag atgattgata attcaatctc tcgcggcctg gcgattttaa aagatgaatt
     2881 tgataataac gattacgtta ggaaatttta attgaatttt tttactgcag cgacgcagat
     2941 ttaataaata ataaaataaa aataaataat catggtagac gtaaaacctg atgaaataag
     3001 cgcgatactt cgccagcaat taagcaactt taaaacaagt tctgatcttg aagaagtagg
     3061 aactgtattg caggtgggtg atggtattgc ccgcatttac ggattaaaca atgctaaatc
     3121 cggagaatta gtaacattcg aaaatggcgt tcgcgctatc gtgttgaacc ttgaagagga
     3181 caacgtgggt gtggtattaa tgggttcttc cgacgagatt aaagaaggtg atactgtgcg
     3241 tcgtacaggt aatatcgctt ctatcaaagt tggtgaaagc atgttaggcc gtgttgtaaa
     3301 tgcactcggc gagcctatcg atggtaaagg tcctttatca ggcgaattat atgaaatgcc
     3361 gttggagcgt aaagcaccgg gggtaattta tcgtcagccg gttaacgaac cattacaaac
     3421 tggtttgaaa ccaatcgatg cgatgattcc tatcggtcgt ggtcagcgtg agttaatcat
     3481 cggtgaccgt cagacaggta aaactgcgat tgcaatcgat accattatta atcagcgtga
     3541 attttatgaa gccggcaaac cggtattttg tatatatgta gcttgcggtc agaaaaactc
     3601 taccgttgct caggtagcta aaacattaac cgaccatggt gcaatggatt atacgattat
     3661 agttgcagct tctgcatctg atcctgctcc attacagttt tatgctccgt tttcaggtgc
     3721 tgcgattggc gaatttttcc gcgatacagg acgtcctgca ttgattgttt atgatgattt
     3781 atctaaacaa gcagttgctt accgtgaggt atcattatta cttcgtcgcc caccgggacg
     3841 tgaagcatat ccgggtgacg tgttctactt acacagccgt ttattagagc gcgctgcgaa
     3901 agtgattaac aacgacgaaa ttgcaagcca aatgaacgac cttcctgctt cattagtagg
     3961 taaagtgaaa ggtggtggta gcttaacagc attaccaatc atcgaaactc aggcaggtga
     4021 cgtatcagca tatatcccaa ccaacgtaat ctcaattacc gatggtcaga tattccttga
     4081 aaccaacttg tttaactcag gtgttcgtcc tgcgattaac gtgggtatct cggtaagtcg
     4141 tgtgggtggt aacgctcaga ttaaatcgat gaaaaaagtt gccggtacat taaaattaga
     4201 ccaggcacaa tatcgtgagt tagaggcctt ctctaaattc ggttctgatt tagatgctgc
     4261 aacaatggcg gtattaaata aaggtcagcg caacgtggaa atattgaaac aaaaacaata
     4321 tgctccaatg tcggttgaaa aacaaattgc aattatttat ctcggaacaa aaggtatgtt
     4381 aggtaaaatt ccggttgata aaataaaagc gtttgaagat gcttatttaa cttatctcga
     4441 aacacaccac aaagcaaccc tcgatgggtt gaaagctggt cagttaacag atgaaattac
     4501 cggtacactt gaaaaagttg ccgctgatat gagcaaacaa tattcggctt aattaataat
     4561 taatcgtgtt tgaaaaaaca catgatataa taagtaatgg caggtaaatt aaaagaagtc
     4621 cgcaccagaa tcacttcggt aatcagcaca cagcagatta ctaaagcgat gaaaatggta
     4681 tccgctgcaa aattgcgtcg cgcacaagac agaattttac agatgcgccc ttatgcatta
     4741 aaaatgggtg aattactcag caatattgct gatgcagccg gtcaggatgt aaaaattgac
     4801 ttcgctcagt cgcgtgaagt taaatcggta ttgcttgtag caattacgtc tgataaaggt
     4861 ttatgcggag cctttaacgc caacgtaatg aagcagatta aaaaaacaat tgaagataat
     4921 tacagccatt tgccaaaagc gaatgtaaaa attatgttca ttggtaaaaa aggatacgat
     4981 gtgtttaaac gcagcgctta taccttaaat accgatttca tgaatttatt tactgctgtt
     5041 gattttgaaa atgtttcacg cgctactgcg tatatcaaag aaggattttt gaaaggtgaa
     5101 tttgatgaag tgcaagtggt ttacaacaaa tttaaaaacg ctgctacgca aattctcgaa
     5161 acagaacagt ttctgccaat taaaaaactg gaacctgttg ccggtgctaa aaatgtaaaa
     5221 tcagattaca tctacgatcc tgaaaaggaa caaatgattc tcgaattagt accgaaaata
     5281 ttaaatactt cattttaccg ttatattctc gacagtaatg cttctgaaca tggtgcacgt
     5341 atgactgcca tggacaaagc atctgacaat gcgaatgagt tattgaaaga attaaaaatt
     5401 tcttacaacc gcgctcgtca ggctgcaatt accaccgagc ttacagaaat tgtgagtggt
     5461 gcggctgctt tgcaggggaa ttaattaaca attgtacaac atttgtgacg gcttatcttt
     5521 atggttaaac ttgagacaat atatacttaa atcctttaaa taggcgttta agagtatgct
     5581 tgatttgtga aatttgtgat agttattaat agagttttgt gacttttttt tgtttttttg
     5641 ggtttttata aataaattag caaaaaagtg acatcgatgt ccacaaaaat gtacacaaac
     5701 attgagaaat cccggatagt attaatgaag atcgcttaca aaagattgat atttgtattt
     5761 ttaggtacat gcatcttttt tattaatgaa gtacacgcgg ctgatagttt gacagtttct
     5821 aatgatttaa atgaaatagc gcaatttgaa atgcaattgc ataatttaga attgaacttg
     5881 gctgaaacaa gagcgttaaa caatgggaga tttgacaatt ttaatttatg gttaactatt
     5941 ttaagtggca tattacttgt tataggtatg ttcactggat ataggacaat ggcaatagca
     6001 aggacagaag cgagagaagc ttttgatgag aaatttaaaa tttattgtga tttaataaca
     6061 aataaatctg aagaggcaga ggctttagta ttgcaaatga gaagtcatca tgaaacttct
     6121 ttagctatta cggaagattt aaatactgct attgataaaa ttttaaaaaa aagtaaaaaa
     6181 gatgaagaag gaaacaaaaa gtgaagtaat tttagaattt atcaaggatt tgaaagaatt
     6241 aatccaaaaa attgaaaata atgaagaaac aatgcaagat tatatcttgt tgcaagaaat
     6301 gtttgaattg tcaaatgttc cggacgatta tattagtgat tgctatctag gatcggggtt
     6361 tcaaaactgg aatgaactct accattcacg gcgcaaaaac tctaggcaag ccccaaacaa
     6421 taagcacttg gaatcggcta tttctaaaat tcgaggaatc agctctgcaa taatttctgt
     6481 aatgcaaaaa tatgttgcag aaaacagtaa cctatataat aaagaccatt aaatttggag
     6541 ttacaaattg cttgaaaaat ttaattattt gaaatagttg taacaatatt ttccgcctca
     6601 acagtattca tgttcttctt tttgccttcc gcaatggctt gttgtgcata ttttttcgac
     6661 aagtcgttat aacctaattt gaaatatatt ttcgcggcgc tcaaattatt aaaatattcc
     6721 ggtttgagtt ctgcacttcg cactgcaaaa ttagctgcct taaccaaagt agatttatcg
     6781 ctgctgtggt cataaatcat atcacttatc cttctcagcg aattggcatc attccacaaa
     6841 tatttttctg cacccgattt tgcagcttgt atgtaagcat cccacttttt gtttttttca
     6901 tataccgata aatagacaaa ggatatacct gcattttttg ggagaactga atgaaaaatt
     6961 attctgaaat atacaatggc tttaagttta aattttggct ttatacccat gaagatttat
     7021 ttgcactcct gttttttttt gcttcttgat cttcgaggtt ttaagttcgt gtggtcaagt
//
LOCUS       DAORKW010000141         7059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_341_length_7059_cov_3.807396, whole genome shotgun sequence.
ACCESSION   DAORKW010000141 DAORKW010000000
VERSION     DAORKW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_341_length_7059_cov_3.807396"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="PLB46_07145"
     CDS             <1..1263
                     /locus_tag="PLB46_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRF76330.1"
                     /translation="LGHCGRKNIRITWGFVKTCLMINYLGQAAWLLHEGGTTLDGRNP
                     FFEMMPHWFVLPGILIATAAAIIASQALISGSYTLINEAMNLNFWPRVLVRQPTDLKG
                     QIYIPSVNIILWLGCVGTVLYFRSSEHMEAAYGFFITITMMMTTVLLTFFMIYRLKWN
                     KWLVFAIVSFFATIELSFFVANVVKIKESWTFLFFAIFIFLTMYVWYSARKINNRFTK
                     FVDIGRHTKQLMELSEDDTIPKFSTHLIYLTKADYRHEIEDKVVRSIFSKKPKRADVY
                     WFVHINRTEHPYTLNYDVSELVDDKVIKINIHVGFRVQLRTELYFKKIVQELVANKEL
                     NLHIRPDGSSKYNAEPDFKFIVIEKFLSVENEFSLKEGLLMNTYFFLKRISQRDERAF
                     GLDKSDVLVEQVPLVYSPAQTVELTRLN"
     gene            1339..2697
                     /locus_tag="PLB46_07150"
     CDS             1339..2697
                     /locus_tag="PLB46_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF76331.1"
                     /translation="MKKMICIVFCLFSLNAVDAKPLAEDSIVQITATDTTLLKKAEPF
                     AFGDFTWVQGNNRQKSALLAGKYFTGGITLDCNYNYAFNKPMDHTNSGSTATFRSNEF
                     NISYIEVGGEINEPESGARGKLMLQFGSRATGIPRNDNTPLRGQFDLYNALRYVTDAY
                     AGIHLNKMHGINIDFGLFKSYVGLLSYNNFENWNYQPSFTSDNTPWFFTGARAQLFPS
                     EKLKIELWLINGWQTYAMFNEMPGVGYQIQWRPNTNTSILSSSYVGHDTPNTPDRLRF
                     HTDNSAVIKYIDKPANNGISKAGFCCTLCEGFESGGGVVPFNGDSLTPAQNFLSLMLY
                     NRFWFGQQHWAWTFGGGTINNPGRYLALLPAGNGVVTQNPGDTFKGWDVSTGVQFMPN
                     EYMTFGVEYVSRHTNIPYFSGPGGVTSPNGWNPPIGEPAGYVADLVKNEERIIFSTIF
                     RF"
     gene            complement(2694..3140)
                     /locus_tag="PLB46_07155"
     CDS             complement(2694..3140)
                     /locus_tag="PLB46_07155"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF76332.1"
                     /translation="MKYENSIVIQLPVARVIELFDNPDNMKYWQKGLQSFEHVSGTPG
                     QPGAVSKLVYKMGNRMVEMKETITERDLPKTFAGIYEAKNVWNYVNNSFIDNGDNTTT
                     YISATEFKFSGLMMKTIALLMPGAFKKQSQKYLEDFMAFAESQPSV"
     gene            3285..4235
                     /locus_tag="PLB46_07160"
     CDS             3285..4235
                     /locus_tag="PLB46_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRF76333.1"
                     /translation="MSLKLLPIALFISFITTLMQQSYAELVWVEKANFGGEARHRGVG
                     FSVGGRGYMGLGHYNSIVDILFEDIWEYDPGSNTWTQKANFMGGKVIQAVAFTIGDIA
                     YVGTGRNPGFVEQKDFYAFDAKTNTWSAIDDFGGSARHGAVAFAINGKGYVGTGTDAS
                     NYKKDLWEYDPVTNNWTQMANLIGAARIAAVGFAIGDKGYIGTGNAFGSTNDFYEYDP
                     VTNTWTTKADVPGPTRQEACGFAMNGYGYIGTGDDYSSGTNYDDFWQYNPGTDTWDTV
                     PEFPGIGRRYMSAFTIGNHGYCGLGTSGTNLKDLWELKEI"
     gene            complement(4232..>7059)
                     /locus_tag="PLB46_07165"
     CDS             complement(4232..>7059)
                     /locus_tag="PLB46_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRF76334.1"
                     /translation="NFIRNNGQLSDMHGDAADNVLFYAEYPYMDVFITTSGLTMLLLE
                     PSFDAFQEKIYHWERVDINLENASILEANISISSPVSTGFNYFTADFPDGIHADEFSA
                     LTIKNIYPGIDWQLSNNGTSGLKYQFVVAPGAYPNDIQLNYLAQNGINITSEGAIKIP
                     TLLGDLIDNAPVIYYAGSEKPIATNYVLNTTSDTSAIIQFNITSFDSTQTIIIDPELY
                     WCTYYGGNKEECFNSVTTDLDGNLIVVGWTDSPNFPVYDAGTYFQGSLATNEDAMIVK
                     FSSDEERLWATFYGGNKEDWAYSVATDNSGNIFVSGETVSNSFPVYNAGTFYQGGLNG
                     DEDVFILKFNSAGTRLWATFYGGDKKEGGYSIATDPSGNIFITGYTESTDFPKLNAGT
                     FYQATMSGVQDAFVLKFTNTGTRIWATYYGGSLYDVGNAITCDLSGNVFVTGETRSTN
                     FPVLDGGGFYDGTMSGDTDVYIIKFSNTGVRTFSTYFGGTDAEYGNSVITDLENNIYF
                     AGWTRSSDMPTLDLGNYYQASLSGLSDAYIVKLDNNGNLLWSTYFGGPGIENAYKWDI
                     LANDFCNNIYLAFTTFSSGMPVYDAGCSSYYDASYEAYGDIFIVRFTDSALITWATYY
                     GYDAEELDACIGSSLIDGKSLYMTGLYNEYDPGAPVPMANPGGVAYYDGTHNGDNEAF
                     IGKFEAVPLTLTTENSNLCFCVDTAVAIPDCGVAPYDYLWSDGQTTATAYGLCPDVYT
                     VTVTDADCNITIGDISIICELPVDIISFNGTINNHTTHLKWTTLSESNIDYYLIEKST
                     DGEIFTVNGAISSVGDNTISTEYVHYDYIATSGTYYYALSSVDFQGNITPYGTITLPV
                     VFDDGDFQLYQINNQLLQLHMHTTSGSEGVFSIYDTKGQLIYIQNLELNEGENNQTIS
                     IPDLPNGIYYATIVTARKQYKGQFMRY"
BASE COUNT         2249 a         1251 c         1336 g         2223 t
ORIGIN      
        1 ttaggacatt gtggcagaaa aaatattcgg attacttggg ggtttgtaaa aacctgtttg
       61 atgattaatt atctgggaca agctgcctgg ttattacatg aaggcggaac aaccctggat
      121 ggcagaaatc cattttttga aatgatgccg cattggtttg ttttaccggg tattttaatt
      181 gcgacagcag cagctataat tgcgtcgcag gcattaatta gtggaagtta taccttaatt
      241 aatgaagcga tgaacttaaa tttttggccg agagtactcg tgcgccagcc aaccgattta
      301 aaaggtcaga tttatattcc cagtgtaaac attattttgt ggctgggatg tgtgggcaca
      361 gttttatatt tccgttcttc agaacatatg gaagctgctt atggattttt tatcaccatt
      421 actatgatga tgaccacagt cttactcacc tttttcatga tatataggtt gaaatggaat
      481 aaatggctgg tatttgctat tgtttcattt ttcgcgacta tcgaactttc attttttgtt
      541 gccaatgtgg ttaaaattaa agagagctgg acatttttgt tttttgccat atttattttc
      601 ctcactatgt atgtgtggta tagtgcaaga aaaattaata accgatttac caaatttgtt
      661 gatattggaa gacataccaa acaattaatg gaattaagtg aagatgatac cattcctaaa
      721 ttctcaacac atttaattta tttaacaaaa gctgattaca ggcacgaaat tgaagataaa
      781 gttgttcgtt ctattttctc caaaaaaccc aaacgggctg atgtatattg gtttgtgcat
      841 attaatcgta ccgaacatcc ttacacatta aattacgatg tttcagaact cgtggatgat
      901 aaggtaataa aaattaatat tcatgttggg ttcagagtgc agttgcgcac agaattatat
      961 ttcaaaaaaa ttgtgcagga gctggtagcc aataaagaac tcaatctgca tattcgtccg
     1021 gacggttctt cgaaatataa tgctgagcct gattttaaat ttattgttat tgaaaaattc
     1081 ttatccgttg aaaatgaatt ttcactgaaa gaaggattgc ttatgaatac ctactttttc
     1141 cttaaacgta ttagtcagcg cgatgaacgt gcgtttggtc tcgataaaag tgatgtattg
     1201 gtagagcagg tgccattggt atattcgccg gcacagacag tggaacttac acgattaaat
     1261 taatttaatt attccaatca ctattggcaa tatccagttt ctgggtaaac gggaaattat
     1321 tgccgtaaaa aaaatgttat gaaaaaaatg atatgcattg tattttgttt gttctcctta
     1381 aatgcagttg atgctaaacc tttagcagag gacagtattg ttcaaatcac agcgacagac
     1441 acaacattgt taaaaaaagc tgaacctttt gcctttggcg attttacctg ggtgcagggg
     1501 aataatcggc aaaaatcagc attattagca ggcaaatatt ttaccggcgg tattacactg
     1561 gactgtaatt ataattatgc atttaataaa cctatggacc atacgaatag cggcagcaca
     1621 gccactttca gaagtaatga atttaatatt tcctatatag aagttggtgg cgaaataaat
     1681 gaacccgaaa gtggtgcaag agggaaattg atgttacagt ttggttcacg agccacagga
     1741 attccgcgaa acgataatac accattgcgc ggtcaatttg atttatataa tgcattgcgt
     1801 tatgtaaccg atgcttatgc aggcattcat ttaaataaaa tgcacggaat taatattgat
     1861 tttggtttat ttaaatcgta tgtgggttta ttatcctata ataatttcga aaattggaat
     1921 taccagcctt cctttaccag cgataatacg ccatggtttt ttaccggtgc aagagcacaa
     1981 ttatttccaa gtgaaaaatt aaaaattgaa ttgtggttaa ttaatggatg gcagacttac
     2041 gctatgttta acgaaatgcc cggtgttggt tatcaaattc aatggcggcc caataccaat
     2101 accagtatat taagcagttc ttatgttggt cacgatacac caaatacacc tgaccgttta
     2161 cgatttcata cagataattc agccgtaatt aaatatatcg acaaaccggc gaataatggc
     2221 atttctaaag ctggattttg ttgtacgttg tgtgagggat ttgaatccgg tggtggagta
     2281 gtgccattta acggagatag tttaacgcct gctcaaaact ttttgagttt gatgttgtat
     2341 aatcgatttt ggtttgggca acaacattgg gcatggacat ttggtggagg aaccattaat
     2401 aatcccggac gttatttagc attattacct gcaggaaatg gagtagttac acaaaacccc
     2461 ggtgatactt ttaaaggttg ggatgtttca acaggtgtgc aatttatgcc aaatgaatac
     2521 atgacatttg gtgtggaata cgtaagcaga cataccaata ttccctattt ttcagggccg
     2581 ggaggtgtta catctccaaa tggctggaac ccaccaattg gtgaaccggc aggatatgtt
     2641 gccgatttgg tgaaaaatga agaacgaatt attttttcaa ctatattcag attttaaact
     2701 gatggctgtg attcggcaaa agccataaaa tcttcaagat atttttgtga ttgtttttta
     2761 aatgcaccgg gcattaataa tgcaattgtt ttcatcatca atcctgaaaa tttgaattca
     2821 gtggctgaaa tataagtggt agtattatcg ccattatcga tgaaactatt attgacgtaa
     2881 ttccaaacat ttttcgcctc gtaaataccg gcaaatgttt tgggtaaatc gcgttcggta
     2941 atagtttctt tcatttcaac catacggtta cccattttat aaaccaattt agaaacggct
     3001 cccggttgtc ccggagtgcc actaacgtgc tcaaatgatt gcaaaccttt ttgccagtat
     3061 ttcatgttgt ccggattgtc gaataattcg ataacacggg caacaggtaa ttgaataaca
     3121 atggaatttt cgtatttcat acagctaaaa taatcaactt attgaaaggt taaaagttgg
     3181 gaaattacat agttataaat tcataaaacg agtggtagaa tttgcaattt tcgcaaaaac
     3241 aggtaaattt aacattgtat tcataataac ttaacacacc aaatatgtcg ctaaaactcc
     3301 tcccaattgc gctttttatc agttttatta ccaccttaat gcaacaatcg tatgccgaat
     3361 tggtatgggt agaaaaggcc aattttggtg gtgaagcaag gcatagagga gttgggttct
     3421 ctgttggtgg acgtggttat atgggattag ggcattataa ttccatagta gatattttgt
     3481 ttgaggatat ttgggaatac gaccccggtt ctaatacctg gacacaaaaa gccaatttca
     3541 tggggggcaa ggttattcag gccgttgcat ttaccattgg cgatattgct tatgttggaa
     3601 ccggccgcaa tcccggattt gtggaacaaa aggattttta tgcctttgac gcaaaaacca
     3661 atacatggtc agcaattgac gattttggcg gttctgcaag acatggcgct gtggcatttg
     3721 caataaatgg aaaaggttat gttggcaccg gtactgatgc ttccaattat aaaaaggatt
     3781 tgtgggaata tgatccggtt actaacaatt ggacacaaat ggccaattta ataggtgctg
     3841 ccaggatagc agcagtaggt tttgccatag gcgataaagg gtatatcgga acaggaaatg
     3901 catttggctc caccaacgat ttttatgaat atgaccctgt taccaataca tggacaacta
     3961 aagcagatgt tccaggtcct accagacagg aagcctgtgg ttttgcgatg aatgggtatg
     4021 gttatattgg aacgggtgat gactactcaa gtgggactaa ttatgatgat ttctggcaat
     4081 ataatcccgg cactgataca tgggatactg ttccggaatt tcctggaatt ggtcgccggt
     4141 atatgagtgc attcacaata ggcaatcatg gttattgcgg cctaggtaca agtggaacca
     4201 atcttaaaga tttgtgggaa ttaaaggaga tttaataccg cataaactgc cctttatact
     4261 gtttacgagc tgttacaatt gttgcataat aaataccatt cggtaaatcg ggaatcgaaa
     4321 tcgtttggtt attttcccct tcgtttaatt cgagattttg tatatatatt aattgacctt
     4381 tagtatcata aatactgaaa acgccttcac ttccgctagt ggtatgcatg tgtaattgta
     4441 atagctgatt attaatttga tataattgaa aatcaccatc atcaaaaaca accggtaatg
     4501 ttatggtgcc ataaggtgta atgttgcctt ggaaatcgac acttgataat gcataataat
     4561 aagtgcccga agtagctata tagtcgtagt gtacatattc tgttgaaata gtattatctc
     4621 caacggaaga aatagcacca ttaactgtaa atatttctcc atctgtcgat ttttcaatca
     4681 gataataatc aatattactt tcactcagtg tggtccactt taaatgagta gtatgattat
     4741 taattgtgcc attaaaactg ataatatcta caggtaattc gcaaataatt gaaatatctc
     4801 caatggtaat attacaatct gcgtcagtaa ctgtgacggt ataaacatcc ggacataaac
     4861 cataagcggt tgcagttgtt tgaccatctg accacaaata atcataagga gcaacaccac
     4921 aatccggaat agcaactgct gtatctacac aaaaacataa attgctgttt tctgtggtta
     4981 aggttaaagg aacagcttcg aatttaccaa tgaatgcttc attatcgcca ttgtgagtgc
     5041 catcgtaata ggcaacacca cctgggttag ccattggaac aggtgcaccg gggtcatatt
     5101 cattatatag acctgtcata tacaacgatt tgccatcaat taatgaagag ccgatacaag
     5161 catctaattc ttctgcatca taaccataat aggtggccca ggtaatgagc gctgaatcag
     5221 taaatcgcac aataaaaata tctccatatg cttcataaga ggcatcgtaa taactcgaac
     5281 aacctgcatc ataaacaggc attccacttg aaaatgtagt aaatgccaaa taaatattat
     5341 tacaaaaatc gttggctaaa atatcccatt tgtatgcatt ttctataccc ggcccgccaa
     5401 aataagtaga ccataataaa ttgccattat tatccagttt aacaatatac gcatctgata
     5461 aaccgctgag tgatgcctgg taataattgc ccaaatctaa agttggcata tcagatgaac
     5521 gtgtccatcc tgcgaaataa atattgtttt ctaaatcggt aataacacta ttgccatact
     5581 ctgcatcggt tcctccaaaa taagttgaaa atgttcttac ccctgtattt gaaaatttaa
     5641 ttatatatac atcagtatca ccactcatag tgccatcgta aaatccacct ccatctaaaa
     5701 caggaaaatt tgtcgaacgt gtttcaccgg ttacaaaaac attaccggat aaatcgcagg
     5761 taatggcatt gccaacatca tataatgaac cgccataata agtagcccaa atacgtgttc
     5821 cggtattggt aaattttaaa acaaatgcat cttgtacacc ggacatggtt gcctgataaa
     5881 atgtgcctgc atttaatttc gggaaatcag tggattcagt ataaccggta ataaaaatat
     5941 ttccacttgg gtctgttgct attgaatatc caccttcttt tttatctcca ccataaaagg
     6001 ttgcccataa tcttgttcct gcactgttaa attttaaaat gaaaacatcc tcatcaccat
     6061 ttaagccacc ctgataaaaa gtacctgcat tataaaccgg aaaggagttt gagacagttt
     6121 cacctgatac aaaaatattt ccgctattat cagtagctac actataagcc caatcctcct
     6181 tattacctcc ataaaaagta gcccataatc tttcttcatc cgatgaaaat tttacaatca
     6241 tggcatcttc gttggtcgct aatgagcctt gaaaataagt gcctgcatca tataccggaa
     6301 aatttggtga atctgtccaa ccaacaacaa ttaaattgcc gtctaaatcg gtggtaacac
     6361 tattaaaaca ttcttcttta tttccgccat aataagtgca ccaatacaat tcaggatcaa
     6421 taataatggt ttgcgtagaa tcgaaggatg taatattaaa ttgtatgatt gcacttgtat
     6481 cgctggttgt atttaataca taattggtag caattggttt ttcacttcct gcataataaa
     6541 tcaccggtgc attatctatt aaatcaccta aaagtgtagg aatttttatt gcgccttcag
     6601 atgttatatt gataccattt tgtgctaaat agtttaattg aatatcattt ggataagctc
     6661 ccggggcaac tacaaattga tattttaatc cgcttgtccc attattgcta agctgccaat
     6721 cgatacccgg ataaatgttt ttgatggtca atgcactaaa ttcatctgca tgaataccat
     6781 ccggaaaatc agctgtgaaa taattaaatc ctgttgaaac aggtgatgaa atacttatgt
     6841 ttgcttcaag aattgaggca ttttccaaat taatatctac ccgctcccaa tgatatattt
     6901 tttcctgaaa ggcatcaaaa gacggctcca ataacaacat ggttaacccc gatgtagtta
     6961 taaatacatc catataagga tattctgcat aaaacaacac attgtctgca gcatcaccgt
     7021 gcatgtcaga cagctgcccg ttgttgcgaa taaaattat
//
LOCUS       DAORKW010000142         6990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_351_length_6990_cov_5.906994, whole genome shotgun sequence.
ACCESSION   DAORKW010000142 DAORKW010000000
VERSION     DAORKW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_351_length_6990_cov_5.906994"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PLB46_07170"
     CDS             <1..1048
                     /locus_tag="PLB46_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76335.1"
                     /translation="YSQYINCENKQTVATEDGTVHGDSCGTCPSCLKAQKFIHPDIHF
                     TFPFVKTDKKEVCADWMVEWRQFMLNTPYGGYNEWITQIIEGENKQGNITARECQEIM
                     RRLSLKNYESTYKIIILWLPEYLGNEGNRLLKILEEPPENTVFILIAHDQERIINTIM
                     SRVQIVKFPRIADQDMIPAIVERFKVGLPQAKKIAALSEGNFNEATQLIDIHEDANTT
                     MIREWMEACYRHNVKALFAWNDRFVKMGREQQKNFFQYCLHFFREVMLLEAGASSLSK
                     LSDAELKTAEGLGKVLGVDRITFILELFDKCIYFVERNVNAKIMMTFVSVTLLKQFQT
                     NSINTRPFYEKWSR"
     gene            1059..2444
                     /gene="ricT"
                     /locus_tag="PLB46_07175"
     CDS             1059..2444
                     /gene="ricT"
                     /locus_tag="PLB46_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRF76336.1"
                     /translation="MSCAGCSVGSNSGIPTGCRSNGHCATGGCNKLNTYDWLRFLPVP
                     TSQQFHIHEISFKNGIRKGFYLNKNKIDVMTGDMVCVETESGFDVGRISLSGELVKLQ
                     MKKRKVNADMQFMPILRKATDNDLERMMEGRAMEGATMLKARVIARELNLDMKIGEVE
                     YQGDKRKATFYYIADDRVDFRELIKLYAKEFRVKIEMRQIGARQEAGKIGGLGSCGRE
                     LCCSTWLTDFKSVGTNAARYQNLSINQSKLSGQCGRLKCCLNYELDTYMDALQEFPRD
                     ADWLQTEFGNARLIKTDIFKKLMVYEYSDGGKQYPLTLIKVREIKQMNQQGQKPEHLE
                     GLKYEPETAVKEVEFVNVIEHVSLKTIERAGQKKRQQNQQNRNKNQQNRNPNQQNRNQ
                     QARPQQQPNKLRDNNTENRNEGDKRNNQQRNPNQQNRQNNPNRNNPNRNNNRNNDNNK
                     RDDNNNQNDKK"
     gene            2449..2928
                     /locus_tag="PLB46_07180"
     CDS             2449..2928
                     /locus_tag="PLB46_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HRF76337.1"
                     /translation="MSLNKHTYYLIPVIALLFILSGCEQSRVFDKNISLPKQGWFYDE
                     AKTFEVEILDTAKSYNLYINVRHTDEYPYNNLWLKLTTTLPDGSVRENNVNIELSAAT
                     GEWSGNCVDGICYNSVLIQHNFSLPQKGKYVFSLEQDMRMNPIPYIMDIGIRVEKFM"
     gene            2936..3742
                     /locus_tag="PLB46_07185"
     CDS             2936..3742
                     /locus_tag="PLB46_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF76338.1"
                     /translation="MPSLRFLLLLLIGVPAGNLFAQTPFVLGETRTIHSTVLNEDRVL
                     NIYLPTYYTDTIQYPVIYLLDGSADEDFIHISGIMQFMNLMQVVPDAIVVGIANVDRK
                     RDFCFPTTIEQDKKDFPTTGGSAKFIEFLRTELQPYIKNNFPTNDTTTLIGQSLGGLV
                     ATEILFKQPAMFNNYIIVSPSIWWDNQSLFTLADSAMNQLPAGDMQIYVAVGKEHPTM
                     VKDAKKLHRLLKHSGKKNLEVHFEQLKNEDHATILHNAVYHAFEQMFSTK"
     gene            complement(3744..4508)
                     /locus_tag="PLB46_07190"
     CDS             complement(3744..4508)
                     /locus_tag="PLB46_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRF76339.1"
                     /translation="MKLITYNLNGIRSAMTKGWVDWIKENGFDVICVQELKADSSQVD
                     TSLFDQLGYHHFWHSAEKKGYSGVAIFTKFKPDNVVIGCGNKAFDAEGRVIRADFGDT
                     SIFSIYFPSGTTGDIRQTVKYQFLDYIYNHLTALQKQGRKLIVCGDYNICNKAIDIHD
                     PKGNKDSSGFLPEEREWMDKLFASGFTDSFRHFNQEPHQYTWWSFRANARNNNKGWRI
                     DYIAVSDTLQSKLISAKIMPDAKHSDHCPQLLELKW"
     gene            4730..>6990
                     /locus_tag="PLB46_07195"
     CDS             4730..>6990
                     /locus_tag="PLB46_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF76340.1"
                     /translation="MIRLIATCCFVFHMCLAVAQPADKPNIIIITYDDMNDWVSNLQG
                     HPQTLTPNLDSIAKLGTTFLNTFCNAPVCAPSRTSWLTGKSPHYTQVYNNEEYISEDF
                     RENFPEDKQIITLPQYLKDSVGYYTVSMNKIFHAQQFKPDFDSTNTDACSRSLSWNDV
                     VLTNNLGGILNAGDELNEDVKNFEWAMIDDSFTTRMKDYTVVDEAALLLDDYTTNPAN
                     FCNKPLMLCVGFHKPHLELYIPEQFFLPYYIKDFYQEPYDFPYNFPSGTFPYNGLVLP
                     PQPGEDYADYDSLGFVGQALAAKGFHDAFKDWADSMALLITINDTLTNNEREDILMKS
                     KMANATMAYLAAINFGDAQLGRLWDALNSHPDFLNNSVIIITSDHGYSLDEKKHWKKN
                     ALWDTDIRVPFYVIDMRNKGERVVKTPAALMDIFPTVCDYIGIQPPVDATGNTYTDGM
                     SLYNIATDTNLIIEKPVLTVYKAEEVKQAGCFEQYSVRNERFHYIKYKSNNIDGDLSC
                     DETQSVIEEELYELGTYREVDPNEWNNLIKDEQYQIVKSFLGQWLPDSILFNRVTYKP
                     LITISNDDCPIGIGDSIGLTLTLYDTTGLPINIPDDFSCNWRSNFNDVIQTGDTVYWA
                     LNDIATILSLDKLIFTLSVEDNTNGYMAGLDIMYFYPDGGENPTIDFTAAINIQSNTV
                     AIDNIVLPVDVDSVVWYYGDGFVYTGFTPPTHTYISYGTFYITCFVYMSEGDKCIVKL
                     LQSVETIDEEQIQ"
BASE COUNT         2314 a         1242 c         1345 g         2089 t
ORIGIN      
        1 ctattctcaa tatattaatt gcgaaaacaa acaaacggtt gctactgaag acggtacagt
       61 gcatggcgat tcatgtggca cttgtccttc ttgtttaaaa gcgcaaaaat ttatccatcc
      121 cgatattcac ttcacttttc catttgtaaa aaccgataaa aaagaagttt gtgccgactg
      181 gatggtggaa tggaggcagt ttatgctcaa cacgccttac ggtggctaca atgagtggat
      241 tacacaaatt atagaaggag aaaacaaaca gggcaatatt acagcccggg aatgtcagga
      301 aattatgcgg aggttatcgc tgaaaaacta cgaatccacg tataaaatta tcatcctttg
      361 gcttcccgaa tatcttggta acgagggcaa caggctttta aaaattttgg aagaaccacc
      421 agaaaatacc gtgtttatct taattgccca cgatcaggaa cgtatcatca ataccattat
      481 gtcgcgtgtt cagattgtga aatttccaag gatagccgat caggatatga ttccggcaat
      541 tgttgaacgt ttcaaagttg gacttccgca ggccaaaaaa atagctgctt tgagtgaagg
      601 caactttaat gaagccacgc aactaattga tattcatgaa gatgcgaata caacaatgat
      661 tagggaatgg atggaagcct gttatcgcca taatgtaaag gcgttatttg cctggaacga
      721 ccggtttgta aaaatgggcc gtgagcagca aaaaaacttc ttccaatact gtcttcattt
      781 tttccgtgaa gtgatgctat tagaggccgg agccagttca ctgtccaaac tttcagacgc
      841 tgaactaaaa actgcggaag gattgggtaa ggtgcttggg gtagaccgca ttacctttat
      901 ccttgaacta tttgataaat gcatttactt tgtagagaga aatgtgaatg ctaagattat
      961 gatgacattt gtttcagtta cccttttaaa acagtttcaa acaaattcaa ttaacacgag
     1021 gccattttac gaaaagtggt cacgctaaat aatatattat gagttgtgca ggttgttcgg
     1081 ttggttcaaa tagtggtatt cctacggggt gccgcagcaa tgggcattgt gccacggggg
     1141 gctgcaataa actcaatact tacgactggt tgaggttttt gcctgttccc acatctcaac
     1201 aattccatat tcacgaaatc agttttaaaa acggcattcg caaaggattt tacctcaata
     1261 aaaataaaat tgatgtaatg accggcgata tggtttgtgt tgaaaccgaa agtggttttg
     1321 atgtgggtag aatttcattg agcggcgaat tagttaaact gcaaatgaaa aagcgcaagg
     1381 taaatgccga catgcagttt atgcctattt tgcgcaaagc aacagacaac gacctcgagc
     1441 gaatgatgga aggcagagcg atggaaggtg caacgatgtt gaaagcacgt gttattgccc
     1501 gcgaattaaa tctcgacatg aaaattggtg aagtggaata tcagggcgat aaacgcaaag
     1561 ccacatttta ttacattgcc gatgaccgcg tggattttcg agaattaatt aaattatatg
     1621 cgaaagaatt tcgtgtaaaa atagagatgc gccaaattgg tgctcgtcag gaagccggaa
     1681 aaattggcgg tttaggcagt tgcggacgcg aattatgttg ttcaacatgg ttaacggatt
     1741 ttaaatcggt tggaacaaat gctgctcgtt accaaaattt aagtatcaac caatcaaaat
     1801 tatccggtca gtgcggtcgt ttaaaatgtt gtttgaatta cgaattggat acttatatgg
     1861 atgccttaca ggaatttcca agagatgccg attggttaca aacagaattt ggcaacgcgc
     1921 gtttaattaa gactgatatc tttaaaaaat taatggtgta tgaatattcc gatggcggaa
     1981 aacaatatcc attaacatta atcaaggtgc gggagattaa acaaatgaat caacaaggcc
     2041 aaaaacctga acatttggaa ggtttaaaat acgagccgga aacagctgta aaagaagtgg
     2101 agtttgttaa cgtaattgag catgtaagtt taaaaacaat tgaacgtgca ggtcagaaaa
     2161 aaagacaaca aaatcaacaa aacagaaata aaaatcagca aaaccgcaac ccaaatcaac
     2221 agaaccgcaa tcaacaggca agaccacaac aacagccaaa caaattgcgt gacaacaata
     2281 cagaaaatcg gaacgaaggc gacaaacgca ataatcaaca gcgtaatcct aatcagcaaa
     2341 atcgtcaaaa caatccgaac agaaacaatc cgaaccgcaa taataatcgc aacaacgata
     2401 acaataaacg cgacgacaac aataatcaga acgataaaaa ataaatcatt gtcacttaat
     2461 aaacatacat attatcttat tccggttatt gctttattat ttattttatc cggttgcgaa
     2521 caatcgagag tatttgataa aaatatttct ttacccaaac aaggttggtt ttatgatgaa
     2581 gccaaaacct ttgaagttga aattctagat accgcaaaat catacaattt atatatcaat
     2641 gttcgtcaca ccgacgaata tccttacaat aatttgtggt tgaaacttac tactacgttg
     2701 cctgatggtt ctgtgcgtga aaacaacgtc aatattgaat tgtcggctgc aaccggcgaa
     2761 tggtcgggca attgtgttga tggaatttgt tataactcag tgcttatcca acacaatttt
     2821 tcgcttccac aaaaaggcaa atatgtattt tcgctcgaac aggatatgcg tatgaatcct
     2881 attccgtata tcatggatat tggaattaga gttgagaaat ttatgtaaca taattatgcc
     2941 atcactacgt tttttattgt tgctgttaat tggtgttcct gcaggaaatc tgtttgcaca
     3001 aacaccattt gtgttaggag aaacaagaac aatacattcc actgttttaa atgaagatag
     3061 ggttttgaat atttatttac ccacctatta tactgacacg attcaatatc ctgtaattta
     3121 tttattggac ggttctgcag atgaagattt tattcatatt agtggcatta tgcaatttat
     3181 gaatttgatg caagtagtac cggatgctat agttgtgggc atcgccaatg ttgaccgcaa
     3241 acgcgatttt tgttttccga caactattga gcaggataaa aaagattttc ctacaaccgg
     3301 aggttcggca aaatttattg aatttctgcg cactgaatta caaccttata taaaaaataa
     3361 tttccccaca aacgatacca caactttgat tggtcaatcg cttggaggac ttgttgctac
     3421 tgaaatttta tttaagcagc cagccatgtt caataattat attattgtta gtcccagtat
     3481 ttggtgggac aaccaatcgt tgtttacact agcggattct gccatgaatc agcttcccgc
     3541 aggagatatg caaatatatg ttgctgttgg caaggagcat cctaccatgg tgaaagatgc
     3601 caaaaaattg catcgtttat taaaacattc cggtaaaaaa aatctggaag ttcattttga
     3661 gcaattgaaa aatgaagacc acgcaaccat tttacacaat gctgtttatc atgcatttga
     3721 acaaatgttc agcacaaaat aatttaccat tttaattcca gcaattgcgg acaatggtca
     3781 ctgtgtttag catcgggcat tattttagcg gaaattaatt ttgattgtaa tgtatcactt
     3841 actgctatat aatcgatgcg ccagcctttg ttattatttc ttgcatttgc gcgaaaactc
     3901 caccaggtat attgatgtgg ttcctgatta aagtgacgga aactatcggt aaaaccggat
     3961 gcaaataatt tgtccatcca ttcacgttct tccggcaaaa atcccgaaga atctttattg
     4021 ccttttgggt catggatgtc aatggcttta ttacaaatat tataatctcc gcatacaatg
     4081 agttttctgc cttgtttttg gagtgcagtt aaatgattgt agatataatc taaaaattga
     4141 tatttaacag tttggcgaat atcaccggtt gtaccggatg gaaaataaat ggaaaaaata
     4201 ctcgtgtcac caaaatcggc acgaattacc cttccttcag catcaaacgc tttgttgcca
     4261 caaccgatta ccacattatc aggtttaaac ttggtaaaaa tagccacacc gctataccct
     4321 tttttttctg ctgaatgcca aaagtgatga tatcccaatt ggtcgaaaag agatgtatcc
     4381 acctgactag aatcagcttt taactcttga acacaaataa catcaaaacc attttcctta
     4441 atccaatcta cccaaccttt ggtcatagca gagcggattc cattgagatt gtatgtaatt
     4501 aacttcattt tgcccaaaat tgaagcgtaa atataaggtt agtcgccata aaatacctat
     4561 accttacctg ttaacaatat agcattccat ataagttaag ttggtaatga cagttgaaag
     4621 tagttcacta attaaacatg ggatttcgcc atgaaattca ggcatttcgc gcatttggta
     4681 cggtacgcac gtaaacaaat cgttaagttt gtgctaatat ttaaacctaa tgattcgcct
     4741 tattgcaaca tgctgttttg tgttccacat gtgccttgcg gtagctcaac ctgccgataa
     4801 gccgaacatt attatcatta cctatgatga catgaacgat tgggtgagca acttacaagg
     4861 ccatcctcaa accttaaccc ccaacttaga ctccattgca aaactcggca caacattttt
     4921 gaatacattt tgtaatgcac ccgtttgtgc gccatctcgt acaagttggt taacagggaa
     4981 atcaccccat tacacccaag tatataataa cgaagaatat atttcagaag atttcagaga
     5041 aaatttcccc gaggataaac agattattac tttaccacaa tatttgaaag acagtgttgg
     5101 gtattatacg gtaagtatga ataaaatatt tcatgctcaa cagtttaaac ctgattttga
     5161 ttcaacgaat accgatgcgt gttcgcggtc actttcttgg aatgatgtgg tgcttaccaa
     5221 taatttaggt ggcattttaa atgcaggtga tgaattaaat gaagatgtta aaaatttcga
     5281 atgggcaatg attgacgatt catttaccac cagaatgaaa gattatactg ttgtagatga
     5341 agctgcattg ctgttagatg attatactac taatccggca aacttttgca acaaaccatt
     5401 aatgttatgc gtgggtttcc acaaaccaca tttagaatta tatatacctg aacaattttt
     5461 tctcccttac tatattaagg atttttatca ggaaccttat gactttccat ataatttccc
     5521 ttccggaaca tttccttata atggattggt attgcctcca caacctggcg aagattatgc
     5581 cgattatgac agtttaggat ttgttggtca ggcgcttgca gcaaaaggtt tccacgatgc
     5641 gtttaaagat tgggcagata gtatggcatt attaattact atcaatgata cacttactaa
     5701 taatgagcgc gaagacattt taatgaaatc gaaaatggcc aatgcaacta tggcctatct
     5761 tgcagctatt aattttggtg atgcacaatt ggggagatta tgggatgccc ttaatagtca
     5821 tcctgatttt ttaaataata gtgtaattat catcacaagc gatcacggtt attcgttaga
     5881 tgaaaaaaaa cattggaaaa aaaacgcgct ttgggatact gatattcgtg tgccttttta
     5941 tgttattgac atgcgcaaca aaggtgaacg agtagtaaaa acacctgcag cattaatgga
     6001 tatcttccca accgtttgtg attatatcgg catacaacca ccggttgatg caactggtaa
     6061 tacgtatacc gatggtatga gtttgtacaa cattgctaca gacactaacc tgataattga
     6121 aaaacctgtg ctaacagtat acaaagcaga agaagtaaaa caagcaggtt gttttgaaca
     6181 atattcagtg cgcaacgaga ggtttcatta tattaaatat aaatccaata atatagatgg
     6241 agatttaagt tgtgatgaaa cccaatcggt aattgaagaa gaattatatg aattaggcac
     6301 ataccgtgaa gttgacccga atgagtggaa taatttaatt aaggatgaac aatatcaaat
     6361 tgtaaaatca tttttaggac agtggttacc cgatagtatt ttattcaatc gcgtaactta
     6421 caaaccgtta ataacaatta gtaatgatga ttgtccgatt ggcattggag actccattgg
     6481 gttaacttta actttgtatg atacaacagg tttgccaata aacattccgg atgatttttc
     6541 ttgtaactgg cgtagcaact ttaatgatgt aatacaaacc ggtgacacgg tttattgggc
     6601 gttaaatgat attgctacca tattatcttt agataaatta atattcactc tttcagtgga
     6661 ggataataca aatggttata tggcgggttt ggacatcatg tacttttatc ccgatggcgg
     6721 agaaaatcca acaatcgatt tcacggcagc aatcaatatt caaagcaata ccgttgctat
     6781 tgacaatatt gttttaccgg tagatgttga ttctgtagtg tggtattatg gtgatggatt
     6841 tgtttatacc ggattcactc cacctactca tacctacata agttatggga cattttatat
     6901 tacttgtttt gtttatatga gcgagggaga caaatgtata gtcaaattat tacaatcggt
     6961 tgaaacaata gatgaagaac aaattcagga
//
LOCUS       DAORKW010000143         6975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_354_length_6975_cov_4.733815, whole genome shotgun sequence.
ACCESSION   DAORKW010000143 DAORKW010000000
VERSION     DAORKW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_354_length_6975_cov_4.733815"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLB46_07200"
     CDS             complement(<1..636)
                     /locus_tag="PLB46_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76341.1"
                     /translation="MKFKIVMMAALIAVLFYQCNPTNKLYTTEEYLGTIDTTAVQSYD
                     YEYADGENYEGDDYSYEGEGEYDYYQTDTAVSPWSSSVYRGAAKRENDLIHTKLEVSF
                     DWQKAWMYGKATLTFKPYFYPTSTLTLDAKGFTVKEVSMITPSGKTPLKYTYNTDSIP
                     DTLQMIIDLGRTFTRTEEYQIYIDYIAKPNDLPKGKTPLKYTYNTDSIPDTL"
     gene            729..1217
                     /locus_tag="PLB46_07205"
     CDS             729..1217
                     /locus_tag="PLB46_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRF76342.1"
                     /translation="MYKVSVNGSGNYEVNGNLVNGETVVADIISLEDRFFHVLSEGKS
                     YNIEVVKADKITKQVTLKVNGNLYEVTAKDKFDLLMDQMGFSAGGGAKIKELKAPMPG
                     LVIEVRVKAGDAVKKGDAVIVLEAMKMENVLKAAGDAVVGSIAVQKGASVEKNQVLIM
                     FE"
     gene            complement(1276..2400)
                     /locus_tag="PLB46_07210"
     CDS             complement(1276..2400)
                     /locus_tag="PLB46_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76343.1"
                     /translation="MKNIMLSCMVLLLIMNTASSQTANKNEELMYERDYDLVTENKTL
                     LVVKIEINEEDINKYKIENKKAFFEHCSKLVDLQNAILWNAVQQHWTLNEKIEFKTIS
                     EIKAIAEGDNASNYLVFGQYTIEQMGTPDNPRSEARFEEAISQSFISLDLDEIPSKRP
                     LSWSDSVYYYHHGPVTGGAYLSLFPLEKLKFTKKSTTLYEYKQPAIFRIALDYIITPE
                     DLYNPLVKYVNDRIADAKENKTITDEIYFTQKKDALKDLTLALPDTYFADLTLMPKRW
                     QEDFCSKEQFKDAYPYKYEILPEAEYLDLVKSKAKGYAIINFAGIRNNADLFITNLNT
                     HELILVTRPPYKVDNDLRVIRIKNNQLEYLKNVLNGLESK"
     gene            complement(2427..3383)
                     /locus_tag="PLB46_07215"
     CDS             complement(2427..3383)
                     /locus_tag="PLB46_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRF76344.1"
                     /translation="MSHLQIAKNVLRFESEQMLKTMEKLDDTFDDAVQAILTAKGKIV
                     VCGIGKSGAVAQKITATLCSTGTPAVFLHAAEAAHGDLGVYADGDPILMISKSGTTEE
                     MLRIIPFFKQANSPVIAIVGNLNSPIARNANFVLDGTVDKEADALNLAPTASTTVALA
                     LGDALAVALMHARGFTEKDFARFHPGGQLGKNLLLKVEDVMHELHEVALVSEKHTFRE
                     LIIAMTQKNLGAACVVDEKNKLIGLITDGDVRRSLLHGTDFNTLTVSNIMTRNPIAIS
                     MFATLKEAVDMMENRSSQLSVLPVVQNEICMGLIRIHDIYQG"
     gene            complement(3439..5091)
                     /locus_tag="PLB46_07220"
     CDS             complement(3439..5091)
                     /locus_tag="PLB46_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76345.1"
                     /translation="MRLHCLLAIVLQCTVITLTAQTNNRLSAYDYILAVNNIKVSLDP
                     SGAIGYSNYNPFYFNVPANDNVPAMYGGFFWVGGKDELGVARTLTPDWYDNKTESRFG
                     PFNYDYYGLTETEYVLLYAPYNRVWLVSKADILYHQLHFNDDDYLIPEAILNWPGNGD
                     IDNGFSAQLAPFIDVNNNFIYEPTLGDYPFIKGDAAIFAMYHDNILYETDAEVNLNTN
                     IEVHVLMYAYESAPTNYLGNTVFVDYTIFNRGDLDFDSLYGGSFVDFAIGANDDDYCG
                     SDSSLNLFYGYNGDLADGPSEPHYTTLPALGCLPVSHQLNSFLFLNSDFSPLGYPDNS
                     VQFLYAMDGKFGDGVQMTTGGNGYSTSATDYTNYMYHGNVNDTTQWTEITESNTPFWR
                     RGVGSIYLGKLSPGNSTCATYAYVFADAGSTTSNTESVDLLKTYSSQTASFYQNTDTI
                     GCLETTYIPLPDSNILSSEVFVVYPNPVETTLYLATGFQANTNFFISIYSMTGSLIFQ
                     QSITNNTDAQTHSININQQATGVYFLVIDYDGYRKSFPVLFK"
     gene            complement(5143..6114)
                     /locus_tag="PLB46_07225"
     CDS             complement(5143..6114)
                     /locus_tag="PLB46_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF76346.1"
                     /translation="MKRILITGAAGFLGSHLCDRFIREGYHVIGMDNLITGDKKNIEH
                     LFGLEQFEFLFHDVSKFVHVPGNLDYILHFASTASPIDYLKIPIQTLKVGSLGTHNLL
                     GLAKAKNARILVASTSEVYGDPLVHPQNEDYWGNVNPVGPRGVYDEAKRFQEAITMAY
                     HTYHGLETRIARIFNTYGPRMRLNDGRVLPAFIGQALRGEDLTVFGDGSQTRSFCYVD
                     DLIEGIYRLLLSDYAYPVNIGNPDEITINEFGEEIIKLTGTSQKLINKPLPVDDPKQR
                     QPDITLAKKLLNWEPKVGRAEGLKITYQYFKSLPQEELYKDIDGKQF"
     gene            complement(6171..>6975)
                     /locus_tag="PLB46_07230"
     CDS             complement(6171..>6975)
                     /locus_tag="PLB46_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRF76347.1"
                     /translation="TESEKAKDMLNRLYAPFVRQGNPIIFMDERSAELTKYAANAFLA
                     TKITFMNEIANLCERLGADVDMVRKGIGSDSRIGKRFLFPGVGYGGSCFPKDVKALSQ
                     MAGAAGYNFSVLKSVMEVNDKQRVILVDKIISQFGNDLSGKTFAMWGLAFKPNTDDIR
                     EAPALYIIDALVQRGATIQAFDPEAMQNVKKEIGDSISYGNNQYDVLKNADALIIVTE
                     WSVFRTPDFELMKSTMRNKMIFDGRNLFELEELKEQGFYYESIGRQVVK"
BASE COUNT         2169 a         1328 c         1227 g         2251 t
ORIGIN      
        1 caaagtgtca ggtatactat cagtattgta agtatatttt aatggtgttt tgcctttagg
       61 taaatcgtta ggttttgcaa tgtaatcgat ataaatttgg tattcttctg ttcgggtaaa
      121 tgttctgccc aaatcgatta tcatttgcaa agtgtcaggt atactatcag tattgtaagt
      181 atattttaat ggtgttttgc ctgatggtgt aatcatcgat acttctttta ccgtaaaacc
      241 tttagcatca agtgtcaatg tagaagttgg ataaaaataa ggtttgaacg taagtgtggc
      301 tttaccatac atccaggctt tttgccaatc gaaagaaacc tctaatttgg tatgaatcag
      361 gtcgttttca cgttttgctg ctccgcgata tactgaactg ctccaagggc taacagcagt
      421 gtcggtttgg taatagtcgt attcaccttc accctcataa ctgtaatcat ctccttcgta
      481 attttcacca tctgcatatt catagtcata actttgaaca gcagttgtgt cgatggtgcc
      541 aaggtattct tcagtcgtat acaatttatt ggttgggtta cactgataaa acaataccgc
      601 aataagtgcg gccatcataa caattttaaa tttcatagtt ttgcttttat aactcgttta
      661 agaggctgta aagttaaaca tttagagata ccttaaacgc acatttcctt aattataata
      721 tcgcgaatat gtataaagta tcagttaatg gcagtggcaa ctacgaagtg aatggcaatt
      781 tggtaaacgg agaaacagtt gtagccgaca tcattagtct ggaggaccgc ttctttcatg
      841 tgctttcaga aggaaaatca tacaacattg aagtggtaaa ggctgataaa atcactaaac
      901 aggtaaccct caaagtgaat ggcaacctat atgaggtaac ggccaaagat aaattcgacc
      961 tgttgatgga ccaaatggga ttttcagcag gaggtggagc aaaaatcaag gaactcaaag
     1021 cgcctatgcc tggacttgtt attgaagtgc gggtaaaagc aggcgatgcc gttaaaaagg
     1081 gtgatgccgt tattgtgctc gaagcaatga aaatggaaaa cgttttaaaa gcagcaggtg
     1141 atgctgttgt tgggtctatt gcagtgcaaa aaggtgcaag cgtagaaaaa aatcaagtgc
     1201 tcattatgtt tgaataaaga acacttgatt aaatactttg tttcacatga agggtgtaaa
     1261 atagttgatg aatacttatt tactttctaa cccattcagt acatttttca gatattccaa
     1321 ctgattattt ttgattctga taacgcgtaa atcgttatcc actttataag gggggcgagt
     1381 taccaaaata agttcgtggg tattcaaatt cgtaataaat aaatctgcat tgttccttat
     1441 tccggcaaaa ttgattattg catatccctt ggctttggat ttgactaaat ccaaatattc
     1501 agcttcgggt aatatttcgt atttataggg atacgcatct ttaaattgtt ctttagagca
     1561 aaaatcttcc tgccatcttt ttggcatgag ggttaaatct gcaaaataag tatcaggaag
     1621 ggctaaggtc aaatctttta atgcatcttt tttttgggta aagtaaattt catctgttat
     1681 agttttattt tcttttgcat cggcaatacg atcattcaca tattttacaa gtgggttata
     1741 taaatcttct ggtgtaatga tataatccaa ggcaattcta aatattgccg gttgtttata
     1801 ttcatataat gtagtacttt tttttgtgaa ttttaatttc tccagcggga ataaagacaa
     1861 gtaagcacca ccagtaacgg gtccgtggtg ataatagtat acgctatcgg accacgacaa
     1921 aggcctttta cttggtattt catccaggtc taatgaaata aaagattggg aaatagcttc
     1981 ttcaaatcgg gcctctgatc ttggattatc aggagttccc atttgctcta tggtatattg
     2041 cccaaatacc aaataattag acgcattatc gccttcagca atggctttaa tttcactgat
     2101 tgttttgaat tctatctttt cattcagtgt ccagtgttgt tgaactgcat tccataaaat
     2161 tgcgttttgc aaatcaacca gtttgctaca atgttcaaaa aatgcctttt tattttctat
     2221 cttatattta tttatatctt cttcgttaat ttcaattttt actacgagta aggttttatt
     2281 ttcagtaacc aggtcgtaat ctctttcata catgagttct tcatttttat tcgctgtttg
     2341 gcttgatgca gtattcatta ttagcaacaa aaccatacac gatagcataa tgtttttcat
     2401 aaagttgatt tttgtataaa taaattttaa ccctgataaa tatcatgtat tctaatcaat
     2461 cccatacaaa tttcattctg cacaaccggc aataccgata attgtgaact gcggttttcc
     2521 atcatgtcta cagcttcttt taaagtagca aacatgctga tagcaattgg attacgcgtc
     2581 atgatattac taactgttaa tgtattaaag tcagtgccgt gtaataaact tctacgtaca
     2641 tcaccatctg taattaatcc aattaattta tttttttcat ctaccacaca ggcggctcca
     2701 agattttttt gagtcattgc gataattaac tcacgaaagg tatgtttttc tgataccaaa
     2761 gctacttcat gtaattcatg cattacatct tctactttaa gtaataaatt tttacctaac
     2821 tggccgcccg gatgaaaacg cgcaaaatct ttttctgtaa atccacgtgc atgcattaat
     2881 gctaccgcaa gggcatcacc aagtgcaaga gcaacagttg tgctggctgt aggagccagg
     2941 ttcaaagcat ctgcttcttt gtccactgtg ccgtctaata caaaattggc gttacgtgct
     3001 atgggtgaat ttaaatttcc aacaatagct ataacaggag aattcgcttg tttgaaaaaa
     3061 ggaatgattc gcaacatttc ttctgtagtg ccactttttg atatcattaa aatcgggtca
     3121 ccatcggcgt aaacacctaa atcaccatgc gctgcttctg ctgcatgtaa aaaaacggcc
     3181 ggcgtacccg tgctgcataa cgtagcagta attttttgtg caactgcccc cgatttgccg
     3241 ataccacata ctactatttt acccttcgct gttaaaattg cctgcactgc atcatcaaat
     3301 gtatcatcca gtttttccat ggtcttgagc atctgctcag attcaaagcg caatacattt
     3361 ttggcaattt ggagatgtga catacacgag taatttaaat gtagtccaaa attacaaagt
     3421 gtaaatcaaa aagcagttct atttaaacaa aacaggaaaa cttttgcggt agccgtcgta
     3481 gtcaataaca aggaaataaa cgcctgttgc ctgttgattg atgttgatgg aatgcgtttg
     3541 tgcatctgta ttattggtga tagattgctg aaaaatcaaa ctgccggtca tcgaataaat
     3601 ggagataaaa aaattggtat ttgcctgaaa accggtggcc aaatataaag tggtttctac
     3661 cggattaggg taaacaacaa aaacctctga tgataatatg ttcgaatcgg gtaacggaat
     3721 ataggtagtt tccagacagc caattgtatc tgtattttga taaaatgaag ccgtttgtga
     3781 agaataggtt tttaataaat ctacactttc agtatttgaa gtagtagatc ctgcgtctgc
     3841 aaaaacataa gcataggttg cacaggttga attgccgggc gataatttac ctaaataaat
     3901 actgccaact ccgcggcgcc aaaaaggtgt atttgattca gttatttcag tccattgtgt
     3961 tgtgtcattt acatttccat ggtacatata attggtataa tcagttgctg atgtactata
     4021 cccattgcct ccggttgtca tttgcacccc atcaccaaat tttccatcca tagcatataa
     4081 aaattgaact gaattatcgg gatatcccaa tggtgaaaaa tcactattta aaaataggaa
     4141 tgaattgagt tgatgtgata ctggcaaaca tcctaaagcc ggtaaagtag tataatgtgg
     4201 ctcagaaggc ccatccgcca agtcgccatt gtagccgtaa aataaattga gtgaactgtc
     4261 tgaaccgcaa taatcatcat catttgcacc aatcgcaaaa tcgacaaaag aaccgccata
     4321 caatgaatcg aaatccaaat ctccacgatt aaaaattgtg taatccacaa atacagtatt
     4381 gcctaaataa ttagttggag cagattcata tgcatacatt aaaacatgca cttcaatatt
     4441 ggtattgaga tttacttcag catcggtttc atacaatatg ttatcatgat acattgcaaa
     4501 aatagcagca tcacctttaa tgaatgggta atctccaagc gtaggctcat aaataaaatt
     4561 attattcaca tcgataaatg gcgctaattg cgcgctaaaa ccgttatcga tatcaccatt
     4621 gcccggccaa tttaatatgg cttccggaat aagataatcg tcgtcattaa aatgtaattg
     4681 atgatacagt atatccgctt ttgatactaa ccaaactcgg ttataaggtg catacaacaa
     4741 aacgtattca gtttcagtta aaccataata atcataatta aacgggccaa atctgctttc
     4801 cgttttatta tcataccaat ccggagtcaa tgttctggct acacctaatt catcttttcc
     4861 acccacccag aaaaaaccac catacatggc tggcacattg tcattggcag gcacattaaa
     4921 ataaaatgga ttgtaattac tatacccaat tgcacctgac gggtctaatg atacttttat
     4981 attgttaact gctaaaatat agtcatatgc acttaaccga ttattggttt gagcggttaa
     5041 cgtaattaca gtacactgca aaacgattgc aagcaaacag tggagtcgca tggaataaaa
     5101 ttacggaact gctaacaaat taccgtaata aaaatgtcaa aattaaaatt gtttaccgtc
     5161 tatatcctta tacaattctt cctgcggcag cgatttaaaa tattggtagg ttatcttcaa
     5221 accctcagcg cgacctactt tcggctccca gtttaatagt tttttagcga gtgtgatatc
     5281 cggctgccgc tgctttgggt cgtcgactgg taatggttta ttaattaact tttgtgaagt
     5341 gccggtgagt ttaattattt cttctccaaa ttcattaatc gtaatttcat cagggttgcc
     5401 aatgttaaca ggataagcat aatcgctcag taataaacga taaatgcctt ctattaaatc
     5461 atctacataa caaaacgaac gtgtttgtga accatcgcca aatacagtta aatcttcacc
     5521 acgtaatgcc tgtccaataa atgccggtaa cacgcgacca tcattcaatc gcatgcgggg
     5581 accgtaagta ttaaaaatac gtgcaattct agtttctaat ccatggtaag tatgataggc
     5641 catggtgatg gcttcctgaa aacgttttgc ttcatcataa acacctcgcg gaccaactgg
     5701 atttacattg ccccagtaat cttcgttttg cggatgtact aatgggtcgc catatacttc
     5761 gctggttgag gcaaccaaaa tacgggcgtt tttcgctttc gctaatccta ataaattatg
     5821 cgtgcccagc gaacctactt ttaatgtttg aataggaatt tttaaataat cgataggaga
     5881 ggcagttgaa gcaaaatgta atatataatc cagattaccc ggaacatgta caaactttga
     5941 aacatcgtga aataaaaatt caaattgctc taaaccgaat aaatgttcaa tatttttttt
     6001 atcgccggta attaaattat ccataccaat tacatggtat ccttcacgaa taaaacggtc
     6061 gcataaatgt gagcctaaaa aacctgctgc tccggttatt aaaatccttt tcaattaggt
     6121 ggtattaaat aaattgtaaa tgaaacatta taattgataa tgcatgtagg ttattttaca
     6181 acctgcctgc cgatactttc gtaataaaac ccttgttcct tcaactcttc cagctcaaac
     6241 aagttacgac catcaaaaat cattttattg cgcatggtac ttttcattaa ttcaaaatcc
     6301 ggtgtgcgga atacgctcca ttcggtaact ataattaatg catctgcgtt ttttaataca
     6361 tcgtattgat tgttgccata actaatgcta tcaccaattt ctttttttac attttgcata
     6421 gcttcagggt caaaagcctg aatggttgcc ccacgttgta ccaatgcatc aataatatat
     6481 aaagcaggcg cttcgcgaat atcatcggta tttggtttaa atgccagtcc ccacatcgca
     6541 aatgtttttc cggaaagatc gttgccaaac tgactaatta ttttatctac taaaataaca
     6601 cgttgtttgt cattcacttc cattaccgat tttaatacac taaaattata acctgctgcg
     6661 ccggccattt gactgagtgc tttaacatct ttcggaaaac aacttccccc ataacctacg
     6721 cccggaaata aaaaacgttt acctatacga ctatcactgc caataccttt tctcaccatg
     6781 tcgacatcag cccctaatcg ttcacaaaga tttgcaatct cattcatgaa ggtaattttt
     6841 gtcgctaaaa atgcattggc cgcatatttt gtcagctcag cactgcgctc atccataaaa
     6901 attattggat tgccctggcg cacaaatggt gcatataagc gattgagcat gtcttttgcc
     6961 ttttcacttt cagtg
//
LOCUS       DAORKW010000144         6973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_355_length_6973_cov_5.103643, whole genome shotgun sequence.
ACCESSION   DAORKW010000144 DAORKW010000000
VERSION     DAORKW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_355_length_6973_cov_5.103643"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1797
                     /locus_tag="PLB46_07235"
     CDS             157..1797
                     /locus_tag="PLB46_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HRF76348.1"
                     /translation="MRLWIELLKNAYYKNDTELETLPNIDINIKCGNSLVSRFAIDAD
                     LKQALKKSKWTIDSYKLAVDTYRNAESKEQKREMERLIADIKSNFRSEISANDPKVKK
                     LYTLNGELVKLSTQQSLFEMSPKEKADWNKKITQLTTETQKLEAEIEEIKSNKIFENA
                     FEWRFEFPEVLNDDGDFVGFDVVIGNPPYGVKPNEMGKEYFSSLYSATDDIYTIFIEH
                     GLKIISENSNVSFINPITWLTGSKYKNTRNLFTKKYSLVKAINLPYDIFADAYVDTGI
                     YCFQRKANEKSEVFEFNPKDKLKIEDLLNIEFKTLNRKVWTSKPDLKINLNESDNSIF
                     WKLDSVSTKLSDFAETVRGILADKSNYNADSGQDFKKVFTGNLNRYILDESFSFIKYG
                     DNLKEKPKSYSFFIGERILIRRIISRQFRMMATYTDKEFVCKKDIYIIKLNENSLSYK
                     YILALLNSKLLSFYKTKNSGSAKKDDFTQITLGDIRQLPIPKIELLNQQIFIKLVDDI
                     LIAKKGNQDTTTMESQIDQLVYQLYNLTEEEIKIVEGN"
     gene            1908..2792
                     /locus_tag="PLB46_07240"
     CDS             1908..2792
                     /locus_tag="PLB46_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRF76349.1"
                     /translation="MFYRRKIILALIQLFDGQIDKIRLQKLLFLFTKKQPKAEYDFIP
                     YRFGCYSYSANADITNMVRKGLLREEDKCIEKIDQTNYVEQLKPNDRSLLQEVKFIYG
                     ALSSTDLIKHTYISFPFYAINSEVAKNILTQNELDNVIRNTPTQDSTMLFTIGYEGIS
                     LEEYLVRLLKNNVKLLVDVRNNPLSMKFGFSKSQIKKYCESIGIQYLHFPEVGIQSAQ
                     RQSLNSQSDYDSLFKVYCENNLTKTTKVQLQIFNLLQTHKRIALTCFEANICQCHRKH
                     LAEAITNLPGFNYEVKHI"
     gene            3224..4543
                     /locus_tag="PLB46_07245"
     CDS             3224..4543
                     /locus_tag="PLB46_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF76350.1"
                     /translation="MLIQFSVSNFKTFKEKATLSLIASNYDKEKREFENVIKETHFGH
                     RILKSAVIYGANASGKSKFMDAFVFMRYFVLNSSKESQQGESIDVDAFKLSTETENEP
                     SEFEIIFLHENTRFRYGFEVNTDAIVTEWLYEKNKNKEIELFYREGQTFNIHDKNFTK
                     GRTVVKEELVRTNALLISVAAQFNEKSAIKVLDWFKKLKPLSGLNQSGYQGFTMVKSE
                     DPKHKAKILNLLKAADLSIKDIQLQKLDVNNLPKDMPNEMKDKITREVREEKAEFISD
                     VLTTHKKYDHLNHAISSVLFSMEEDESSGTSKFFALTGPILDVLENGYTLVVDELDSK
                     LHPNLVCKIVALFNSKGFNKKNAQLIFNTHDTNLLSSGLFRRDQIWFTQKNKFGAAKL
                     YSLADFKSDEVRKNDPFEENYIRGKYGAVPFLSNFDNLNFSLAENEK"
     gene            4533..5231
                     /locus_tag="PLB46_07250"
     CDS             4533..5231
                     /locus_tag="PLB46_07250"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HRF76351.1"
                     /translation="MKNKEAIQKQKREQHRALMKAQRKSEPVLSRAAPVLDEKPTILI
                     VCEGENTEPSYFNQFRVTSATVKSVGAGCNTVSLVARAIQISKEGNYDQVWCVFDKDN
                     FPDVNFNNAIKLAIKNNFGIAYSNQAFEYWLILHFNDHQGGGIKRQDYNQMINDLIKP
                     FNIKYDGNGNKLIEEAFFEILDGIDSKTQNKRVELAITRAEKVYNQFDHTNPAKEESS
                     TTIFRLVQELLKYI"
     gene            5271..5885
                     /locus_tag="PLB46_07255"
     CDS             5271..5885
                     /locus_tag="PLB46_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76352.1"
                     /translation="MPRDPIEISAITLIDKALEFADMLKAHPDWNLNQLKLNTSYEIR
                     YRQLDVLIKYFIPEIYHKHDLKTSLQRVITGNFIEMRPITTYKNLLPEMDKTILSAKI
                     TMPQKQPWRIDHILSNYCDLIAFKQKMNTVLNHNMDYMEISYPYLFSVEISDQIIRNI
                     DTSVMDKFIGRVIDPLGRIVHKATLIKDFNYPEEDVYSVDLDNW"
     gene            complement(6436..>6973)
                     /locus_tag="PLB46_07260"
     CDS             complement(6436..>6973)
                     /locus_tag="PLB46_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76353.1"
                     /translation="LALYPKVFFYNVNKNVVKDSYTSYNAKLTTLCSGFDDGYNIDTK
                     WTSIACIQETKLAYDTCIYSTQYYPLDISYIFMYTTFGFCLLVRLKHRFRRFKYNTLL
                     FSALKYALIVASVADFFENTGLLLYLNFDIDGALYLNFVANKIKLIFIVVVALSLGTL
                     CLLNFVIDIRRFYKLIRS"
BASE COUNT         2629 a         1092 c         1119 g         2133 t
ORIGIN      
        1 attgtcaccg acgaagatgc cgaattattt gaatacaatc ccaataataa agaaagtcaa
       61 cgcattcaag aaacactctt ccacgaaaaa caaactatta ttgaaaactg cctttttggt
      121 gtcgacatta atacgaattc ggtgaaaata tgccgtttgc gtttatggat tgaattatta
      181 aaaaatgcgt attataaaaa tgatacggag ttagaaacac tacctaatat cgatatcaat
      241 attaaatgtg gcaactcatt ggtaagccgc tttgcgattg atgcagattt gaagcaagca
      301 ctgaaaaaaa gcaaatggac aatagatagt tacaaattag cagttgatac ctaccgcaac
      361 gccgaaagca aagaacaaaa gcgcgaaatg gagcgcctca ttgctgatat aaaatccaat
      421 ttcagaagcg aaatatcggc taacgaccca aaagtaaaaa agctctatac tctaaatggt
      481 gaattagtaa aactcagcac ccagcaaagc ctttttgaaa tgagccctaa agaaaaagcc
      541 gactggaaca aaaaaataac ccaactcaca accgaaaccc aaaaattaga agccgaaatt
      601 gaagaaataa aatcaaacaa aatatttgaa aacgccttcg aatggcgatt tgagtttccg
      661 gaagtgttga atgatgatgg ggattttgtg ggatttgatg tggtgattgg gaatccgccg
      721 tatggtgtta aaccaaatga aatgggaaag gaatacttca gctcattgta ttcggcaaca
      781 gatgatattt atactatatt tattgaacac ggccttaaaa ttatcagcga aaattcaaac
      841 gtaagtttca ttaatcctat tacttggtta acaggatcta aatataaaaa cacaagaaat
      901 ttattcacaa aaaaatattc tttagtaaag gcaattaatc ttccttacga tatttttgct
      961 gatgcttatg tcgatactgg catttactgc tttcaaagaa aggcaaatga aaagtcagaa
     1021 gtatttgaat ttaatcctaa ggataaatta aaaattgaag atttactaaa tatagaattc
     1081 aaaactctta accgtaaagt ctggacatcc aaaccggatt tgaaaattaa ccttaacgaa
     1141 tcagataatt caatattttg gaaacttgat tcagtatcaa ctaagctttc agattttgct
     1201 gaaaccgtga gaggaatatt ggctgataaa tcgaactaca atgctgattc gggtcaagat
     1261 tttaaaaagg tatttacagg taatttgaat cgatatattt tagatgagtc ttttagtttt
     1321 attaaatatg gtgataactt aaaagagaaa ccaaaaagtt atagtttttt tataggcgaa
     1381 agaattctaa ttagaagaat aataagtcgt cagtttagaa tgatggcaac atacacagat
     1441 aaggaatttg tatgcaaaaa agatatttac ataatcaaat tgaatgaaaa cagtctcagt
     1501 tataaatata tattagcgtt gctcaattca aaattgcttt ctttttacaa aactaaaaat
     1561 tctggttcag caaaaaaaga tgattttaca caaattactt tgggtgatat aagacaactt
     1621 ccaattccta aaattgaact attaaatcaa cagattttta ttaaattagt tgatgacatt
     1681 ttaatagcta aaaaaggaaa tcaagacaca actactatgg aatcccaaat cgaccaatta
     1741 gtctaccaac tctacaacct cactgaggaa gaaataaaaa ttgttgaagg taattgaggc
     1801 attctatatt tataagccct taaacaatta atacacgact aaattgctga caatggtagc
     1861 tctgctatgc tcatagaata tttccaaaat aaaagtaact ttgcaaaatg ttttaccgcc
     1921 gaaaaatcat acttgcttta atacaactat ttgatggcca aatagataaa attcggttac
     1981 aaaaattact ctttttattt acaaaaaagc agcctaaagc tgaatatgat tttatacctt
     2041 atcggtttgg ttgttattcc tattctgcca atgctgacat taccaatatg gtgcgcaaag
     2101 gtttgttgcg tgaggaggat aaatgtattg aaaaaattga ccaaacaaat tatgttgaac
     2161 aactaaaacc taatgacagg tcacttttac aagaagttaa atttatttac ggtgcattga
     2221 gttcaacaga tttaataaaa cacacttata ttagcttccc attttatgcg ataaatagtg
     2281 aagtagctaa aaacatttta acccaaaatg aattggacaa tgtaatccgc aatacaccaa
     2341 ctcaggatag tacaatgctt tttaccatcg gatatgaagg aatttcattg gaggagtatt
     2401 tggttcgttt gttaaaaaat aatgtcaaac tcctggttga tgttaggaac aatccgttaa
     2461 gtatgaaatt tgggtttagt aaaagtcaaa ttaagaaata ttgtgaaagt ataggcattc
     2521 aatatttaca tttcccggaa gtaggaatac aatctgcaca aaggcaatcg ttaaatagcc
     2581 aaagtgatta cgattcactt tttaaagtct actgcgaaaa caatcttacc aaaacaacaa
     2641 aggttcaatt gcaaatattc aatttattgc aaacacacaa aagaattgca ctaacttgtt
     2701 ttgaggcaaa tatttgtcaa tgccaccgta agcatttagc tgaagctatt acaaaccttc
     2761 ccggctttaa ctatgaagta aaacacatct aaagtatggc attaacgaaa gtattgatcc
     2821 aaaaatagat ttgcaaacga aattgttcta aaaagtttag tattcaaatt taatttccaa
     2881 tcggcaaagt agccgaccaa ctctacaaca agtctgaacg agagataaaa atttttagcc
     2941 gataaaaagt gacaaagtat actgcttaat aaaaaacaca tttaatttat atatcattgt
     3001 ttttcaacta tttatacatt ttttttgttg aaatttgtga catttcagcg tctcgcctac
     3061 ttatgttttt gccaatggaa ttactaaatc gaagggaaat acaactattt taagcaattt
     3121 aaataacctt ttaggcaata tttacacaaa aacagcattt attttaaact tttttctaaa
     3181 ataaagtgta tttttgccta taactcatta aaaagcctat tttatgctca tccaattttc
     3241 agtatcaaat ttcaaaacct ttaaggaaaa agccactttg agcttgattg cgtctaatta
     3301 tgacaaagaa aagcgggaat ttgaaaatgt tataaaagaa acacatttcg ggcatcgtat
     3361 attaaaaagc gctgttatct atggtgctaa tgcaagtggg aagagtaaat ttatggatgc
     3421 ttttgttttt atgcgatatt ttgttttgaa cagttctaaa gagagccagc agggagaatc
     3481 aattgacgta gatgctttta aacttagcac agaaacggaa aacgagcctt ccgaattcga
     3541 gattattttc ttacatgaaa atacacgatt tagatatggt ttcgaagtaa atactgatgc
     3601 cattgtaacc gaatggttat atgaaaaaaa taaaaacaaa gaaattgagt tattctatag
     3661 agaaggccag acctttaata tacacgataa gaatttcaca aaagggcgca ctgtagttaa
     3721 agaagagttg gtgagaacaa atgccttatt gatttcggta gcagcacaat ttaatgaaaa
     3781 gtcagccatt aaagtacttg attggtttaa aaaattgaaa cccctttcag gacttaacca
     3841 atctggatac caagggttta ctatggtaaa atctgaagac cctaagcata aagctaaaat
     3901 ccttaattta ttaaaagctg ctgacctaag cataaaagat attcaactgc agaaattaga
     3961 tgtaaacaac ctcccaaagg atatgcccaa tgaaatgaag gacaaaataa caagagaagt
     4021 tcgtgaagaa aaggctgaat ttatttcgga tgttttaaca acacataaaa aatatgacca
     4081 cctcaaccac gcaatatcaa gtgtactatt ttcaatggaa gaagatgagt catcaggcac
     4141 atcaaaattt ttcgcgttaa ccggacctat tttagatgtg ctggaaaatg gttacacact
     4201 tgttgttgac gaattagact cgaaattaca tcccaattta gtatgtaaaa ttgttgcctt
     4261 atttaactct aaagggttta acaaaaaaaa tgcacaatta atttttaata ctcatgacac
     4321 aaatctgtta agttcaggat tatttcgtcg tgatcaaatt tggtttaccc aaaagaataa
     4381 atttggagca gcaaaattgt attctttagc tgatttcaaa tcagatgaag ttcgaaaaaa
     4441 cgaccctttt gaagaaaatt atattagagg aaaatatggt gctgtcccat ttttaagcaa
     4501 ttttgataac ctaaattttt ctttggctga aaatgaaaaa taaagaagcc attcaaaagc
     4561 aaaaacgaga acagcatcgt gcactgatga aagcgcaaag gaaatcagaa cctgtattat
     4621 cccgcgcggc tcctgtgtta gatgaaaaac caactatttt aattgtttgc gaaggtgaaa
     4681 atacggaacc ttcgtatttc aatcaattta gagttacttc ggctacagtt aaatcggtgg
     4741 gagccggatg taatacagtt tctttggttg cacgtgctat ccaaatatca aaagaaggta
     4801 attacgacca ggtttggtgt gtattcgata aggataattt tcccgatgtc aatttcaata
     4861 acgcaattaa attagctatt aaaaacaact ttggaattgc ctattccaac caagcctttg
     4921 aatattggtt gattcttcat tttaatgacc atcaaggggg tggcattaaa aggcaagatt
     4981 acaatcaaat gattaatgat ttaattaaac cgttcaatat taaatatgat gggaacggca
     5041 ataaactaat tgaggaggct ttttttgaga tactagatgg tattgattca aaaacccaaa
     5101 acaaacgggt tgaattagca attacaagag ctgaaaaagt atataatcaa tttgatcaca
     5161 ccaaccctgc aaaagaagaa tcttctacaa ctatatttag actagttcag gaacttttaa
     5221 aatatatata acttttaatt tatacgtgcc cctttaataa caaaataaat atgcctcgcg
     5281 acccaattga aatttccgca attacactta tcgacaaagc tttggaattt gccgacatgc
     5341 taaaggccca tcctgattgg aatttaaatc agttgaaatt aaatacaagt tacgaaatac
     5401 gatacagaca gcttgatgtt ctaataaaat attttattcc ggaaatttac cataaacacg
     5461 atttaaaaac tagtttacag agagttataa ctggtaattt tattgagatg cgaccaatca
     5521 ccacctataa aaacctttta ccggaaatgg ataaaactat tttatctgca aaaataacta
     5581 tgccccaaaa acagccatgg cgaattgacc atattttaag taattattgt gatttaatag
     5641 cttttaagca aaaaatgaat accgtattaa atcacaatat ggattatatg gaaattagtt
     5701 atccgtattt attttctgta gaaatatctg accaaataat tcgtaacatt gatacaagtg
     5761 taatggataa atttataggt agggtaattg accctcttgg tagaatagtg cacaaagcaa
     5821 ctttgataaa ggattttaac taccccgagg aagatgtgta ttcagttgat ttggataatt
     5881 ggtgaaataa tgtattcatt aacaattaac cttctttatt tgaattattt cagtcgaact
     5941 agcgctgatt taaacgcaat aaaacaaaag ttctgatgcc ccaactaatc tttaaacaca
     6001 aagctgattt acagattaaa caaacaaaat acgcattcgc tttttttgct ttactaccca
     6061 caaagtaaag ttttaccctt cacacatgcg ccatttgagt taatttaaca gattcacctc
     6121 caacatcagt tgctttcgta ggtttgctaa actaataaat aaaatacata ttttaatcag
     6181 tttttaaaat tttgctttaa attacattga tgaaaatacc tttaaatcaa ttcgaacagc
     6241 ttatcgatga acaggtcatg gaacgggggt taaaatattt aaaaaaaggt tttgttactg
     6301 aatacgaata aattagtccg ggtatatagg aagcagaaat tgagggcact gaaatattta
     6361 ttcaattgaa tttacactta tatacgaatt ttgccgtata aactaattga agataattac
     6421 ttttttacat ttgttttatg acctaataag cttataaaac ctccgaatat caattacgaa
     6481 gtttaacaaa cagagtgtgc caagtgataa tgcaactaca acaataaata taagttttat
     6541 tttattggcg acaaagttga ggtataaagc accatcgata tcaaagttta aataaagcaa
     6601 caaccctgtg ttctcgaaga aatctgcaac acttgcgaca attaatgcat attttaatgc
     6661 actgaatagt agtgtattgt atttaaatct cctaaatcgg tgctttagtc ttacgagcaa
     6721 acaaaacccg aaagtggtat acataaaaat ataactaata tccaaagggt aatattgagt
     6781 tgagtatata catgtatcgt aagcaagttt ggtctcctgt atacatgcta tacttgtcca
     6841 cttagtatct atattatacc catcatcaaa tccagaacaa agcgtagtca attttgcatt
     6901 gtagctggta taactatcct tcactacatt tttatttaca ttgtagaaga acactttagg
     6961 gtaaagtgcc aat
//
LOCUS       DAORKW010000145         6942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_358_length_6942_cov_4.524757, whole genome shotgun sequence.
ACCESSION   DAORKW010000145 DAORKW010000000
VERSION     DAORKW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_358_length_6942_cov_4.524757"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLB46_07265"
     CDS             <1..411
                     /locus_tag="PLB46_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76354.1"
                     /translation="KGWLTLLRNRWCTFTEIISHDIDFNDKTPQEQLIHYRLMLADSV
                     SQWWEKSKATWTRFSALKTALASTNKRSQELALEYLRFDNTICEGLTLESYKTELEPL
                     VKAIKNSNNSNADQSKYLLEDKEFYWLKVRKNTQ"
     gene            595..1416
                     /locus_tag="PLB46_07270"
     CDS             595..1416
                     /locus_tag="PLB46_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76355.1"
                     /translation="MRNTLLLIFTLFFAGQTAYSQYVSENYYNFDNEINEILLQTDKG
                     EINRIICKFKIFVQPNKKKDLKEVIEFEFLNDTIIMALTYWEYKDYWVNRSGKLVNID
                     YDQENSVVRKDNSPFIDSAGYKVFYTVLNNVTDTTYYYIRKKIDNGGKSVETIYRETS
                     RKLGQETTFYKKDCIGDTCYTNIYQLTSGDRILLYEIKEWKVWANNRVIDAPSITIKY
                     IYETNGNITKSVTTSNSVYLYDSIGRLASVTKFFFLDYDEKWYQKHMMRVKYISR"
     gene            1663..2586
                     /locus_tag="PLB46_07275"
     CDS             1663..2586
                     /locus_tag="PLB46_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76356.1"
                     /translation="MANTNKHKLFGNRLLQYSALSGAFILGKPDIIFAEVVYTNVEPD
                     VVLDEDDEGFFLDFNDDLDSDVHFLNVSNYFEGYWTSGSINYVFPTAEQRIKIMALGN
                     SIAGSLVTYSTYSSGTIHRIFPYALEEDLIDNALQFQNAYNQTLNFRNIYYFFGTYSS
                     YGGNWFPESIDHYLGIRFNDADEITHYGWIRCDVKDDGRTLVIKDYAYETEPNYPIIA
                     GDTTHYVNINTDENQIDATAYSFGKDIYVISKTFQIIEIKVYDLTGREINNSLMQKEM
                     EIIDMSKCVPGVYFVKLIQDNRTFDKKVIIE"
     gene            complement(2806..4683)
                     /locus_tag="PLB46_07280"
     CDS             complement(2806..4683)
                     /locus_tag="PLB46_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF76357.1"
                     /translation="MTTQTHFILATIVAMTLSMQLHAQILFDYKGDEDGVAEAVAAGL
                     IFDLLDEVNGPIHSEVWCSSGLGSTNWELSDGPFNTGFSAMQIVLIPESCRSMTVTSF
                     QFTIRRDAAGPQLNRMAYSIDGGVTWVDAGSDFATNANPCEVGEVYNWDVPDFTTTST
                     LIFRIYGWNADNSAGQQHLLDGFISGTTSFVGPFTYYADVDADGYGNAGSTTTACSAP
                     LGYVANSLDCDDANSGIKPGATEICNSIDDDCNGTADDGLTFTDYYADNDGDTYGDPA
                     TSISTCDGAPVGYITDNTDCDDDVAVINPTTVWYADADADGYYTGPGITQCVYPGPGY
                     YFDGFAGGGDCNDADPAINPGATEICGNAIDENCDGYIEALAVYANIFSENPVCKPNS
                     VILGASPSGVGNTYQWYRNGTIIPGATSENLNVTALRGHYQVMVSNGSCTEISDSVFV
                     RIYNPNDPVINYATTDLCVTNPIKLKATFGFAPDHSYQWYKNDILIPGATSAKYNATE
                     AGVYTVNVYADFLGCNFWSAGVELSEACRLDEENTNLQASIYPNPNNGTFVMSLNSAL
                     SIQDATVQITNLLGEVIYTTVISASTTQLELSIPSPAAGIYTATIISGDTHTQLQFVV
                     Q"
     gene            4997..5821
                     /locus_tag="PLB46_07285"
     CDS             4997..5821
                     /locus_tag="PLB46_07285"
                     /inference="COORDINATES: protein motif:HMM:NF020962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HRF76358.1"
                     /translation="MISKSNYSRLEHWIEECQSKGKLAFNLAELRQRFLADTEIALKR
                     GLDRLSEKEKVVSIFKGYYIIIPPQYLSKGILPVTMFIDGLMKFLDRKYYVGLLNAAA
                     LHGASHQQPQEYFVVTEYPIMRPTQKKGLKINYISRRQLPPESLTEKMKTETGYITVS
                     NPLLTAIDLITYEKKVGGLNRASTVISELFETIKRNDINEALINYASVSSLQRLGFVL
                     DEVINKKEIADKILSLCKKTGVNFYLIPLKASGNNYKEHLNEKWKLMINTKIETDF"
     gene            5873..6664
                     /locus_tag="PLB46_07290"
     CDS             5873..6664
                     /locus_tag="PLB46_07290"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRF76359.1"
                     /translation="MIPQAYITEWSNNVPWQTNEQVEQDLVICRALVEIFKDDFLSDS
                     LAFRGGTALHKLYLQPQPRYSEDIDLVQIQAGSIGKVIDHIRKALSFLGKAKVETGET
                     MATMKFRFDSEFPPIVSLRLKIETNCREHFTVLGWEKKFFEVKSSWFTGECNLITYRL
                     EELLGTKLRALYQRRKGRDLYDLWKAFTASNLNPDLLIKCYKEYMIFSLEKKIPTKKE
                     FLMNLEEKKVDADFLGDTAALLRPDEKYNHDEAFRIIIDTLIERM"
BASE COUNT         2392 a         1203 c         1305 g         2042 t
ORIGIN      
        1 aaggggtggc tcactttgct ccgaaatagg tggtgcacgt tcaccgaaat aatcagtcac
       61 gacatcgact ttaatgacaa aacaccacag gagcaactta tccactatcg cctgatgctc
      121 gccgacagcg tttcacaatg gtgggaaaaa agcaaagcca cctggacgcg cttctccgca
      181 ctcaaaacag ccctcgcaag caccaataaa agaagccagg aattagcatt agaatactta
      241 cgttttgata atacaatatg tgaagggcta acacttgaat cctataaaac tgaactggaa
      301 cccttggtta aagcaattaa aaacagtaac aattccaacg cagatcagtc taaatatttg
      361 ctcgaggata aggaatttta ttggttgaag gtgaggaaaa atacgcaata gtgtcgttat
      421 tgcgttagga gggtttgagc aaacaagtct gtaaaaggaa ttggatactt attttttcaa
      481 aaaaatgtta ttttgtgata tgtgctttac ggtttagctg caggttaatc aaaagttgct
      541 actcctaatc cggatattcg cctagaacaa aactgttaaa attaattata acaaatgcgt
      601 aacacactcc ttttaatatt cactttgttt tttgccggac aaactgccta ttcccaatat
      661 gtttctgaaa actattacaa ttttgataac gaaataaatg aaatcctttt acaaactgac
      721 aaaggtgaaa ttaataggat aatctgtaaa tttaaaatat tcgtccaacc gaataaaaag
      781 aaagatttaa aggaagtgat tgaatttgag tttctcaatg acacaatcat aatggcactg
      841 acttattggg aatataaaga ctattgggtt aataggagtg gaaaattagt aaatatagac
      901 tatgaccagg aaaattccgt tgtaagaaaa gataattcac catttattga ttccgctggt
      961 tataaagtct tttataccgt attgaataat gtaacagata caacttacta ctacatacgc
     1021 aaaaaaatag acaatggtgg aaaatcggta gaaacaattt atcgggaaac ttcgcgtaaa
     1081 ttaggtcagg aaactacatt ctataaaaaa gattgtatag gagatacatg ttacactaat
     1141 atatatcaat taacaagtgg tgaccggata ttattgtatg aaataaaaga atggaaggtt
     1201 tgggcaaata acagagtaat cgatgcgccg tccattacga taaaatatat atatgaaact
     1261 aatggtaata taactaagtc cgtaactact tctaattcag tttatttgta tgacagcatt
     1321 ggtaggttgg cttcagtgac gaaatttttc tttttagact atgacgaaaa gtggtatcag
     1381 aaacacatga tgagagttaa atatatttcg cgttaaatgt tattattgca tatttaacac
     1441 attttttcgg caactttata gtcaagcttg aaatgtgttt tactccattg actgaaaatc
     1501 cctccgtgcc gcctcgctgc tggtagaaat cacatttaaa gattttagta tcaagactaa
     1561 caaaatactg aacataaaca cataaattta catcaacaaa attacaccct tattcaaaac
     1621 cggctgtgat gacctcgact ttgtttaaac ttcagatgag ttatggcaaa tactaataaa
     1681 cacaaattat tcggaaatag gctattgcaa tattctgcat tgtcaggtgc atttatttta
     1741 ggtaagccgg atattatatt tgctgaagtt gtttacacaa atgttgaacc tgatgttgtg
     1801 ttggatgaag atgatgaagg cttttttttg gactttaatg atgacttgga tagtgatgta
     1861 cattttttaa atgtatctaa ttactttgaa ggttattgga cttccggaag cattaattac
     1921 gtttttccta ccgcggaaca acgaattaaa attatggctt taggaaatag cattgcaggt
     1981 agcttggtaa cttatagcac ctattcttca ggaacaatac atagaatatt tccttatgct
     2041 cttgaagaag acctaattga taatgcatta caattccaaa atgcttataa ccaaacccta
     2101 aattttagaa atatttatta tttttttggg acatatagca gttacggtgg gaactggttt
     2161 ccagaatcaa tagaccatta tttaggcatt cgatttaatg atgctgatga aattacgcat
     2221 tacggatgga tacgctgcga tgtaaaggat gacgggcgta ccttagtaat taaagattat
     2281 gcatatgaaa ctgagcccaa ttatccaata attgcgggtg ataccacgca ttatgttaac
     2341 atcaataccg atgaaaatca aatcgatgcc actgcgtata gttttggtaa ggatatttat
     2401 gtcatttcaa aaacatttca aattattgaa attaaagttt atgatttaac tgggagagaa
     2461 attaataata gccttatgca aaaagaaatg gaaataatcg acatgtctaa atgtgttcca
     2521 ggagtttatt ttgtaaaatt aattcaagat aatagaacgt ttgataaaaa ggtgattatt
     2581 gagtaaatta tttggaaaac cgattaaacg taattccgga gatcgaatta tatttcaata
     2641 caatagacga agaatcgcat tgtaaatcat caggaggaat ccgcagctta cccaattata
     2701 tgctcaatta aataaaaaga ttattaaaat aacttaaccc tactttcaag tattaacaaa
     2761 aaaaatgccc acaaatagcg ggcattttat aattcgataa attaattatt gcacaacaaa
     2821 ttgcaattgc gtgtgcgtat ctccactgat gatggttgct gtgtaaattc cggcagcagg
     2881 actcggaatg gataattcta attgagttgt tgatgctgaa ataactgttg tatatatcac
     2941 ctcaccgagt aaattagtaa tttgaactgt tgcgtcctgg atggataagg cggagttcaa
     3001 cgacataaca aatgttccat tgttcggatt tggataaatg gaagcctgaa ggttagtatt
     3061 ttcttcatcc aacctgcatg cttcggagag ttcaactccg gctgaccaaa aattacaacc
     3121 gaggaaatct gcatacacat ttactgtata tacacctgct tctgtggcat tatattttgc
     3181 actggttgct ccgggaataa ggatatcatt tttataccat tgataagaat ggtcgggcgc
     3241 aaatccaaaa gtagctttta gcttaatcgg gtttgttacg cataaatcgg tggtggcata
     3301 attaattacc ggatcatttg ggttataaat tctaacaaaa acactatcag aaatttctgt
     3361 gcaagatccg ttacttacca ttacctgata gtggccgcga agtgctgtga catttaagtt
     3421 ttcgctggtg gctcccggga taattgttcc attgcgatac cattggtacg tgttgccaac
     3481 accactcgga cttgcaccta agattaccga attaggttta caaaccggat tttcggagaa
     3541 gatattcgca taaactgcta atgcttcaat atatccatca caattttcgt cgatagcatt
     3601 accacaaatt tcggttgctc cgggattgat ggcaggatct gcatcattgc aatcgccacc
     3661 accggcaaaa ccatcaaagt aatatccggg gccgggataa acacattgtg taatgcctgg
     3721 accggtataa tatccatctg catctgcatc tgcataccaa acggttgtcg gatttattac
     3781 tgcgacatcg tcatcacagt ctgtattatc cgttatataa ccaacaggag cgccatcgca
     3841 agttgaaata gaagttgcag gatcaccata ggtgtcacca tcattatctg cataataatc
     3901 agtgaatgtg aggccatcat ctgctgtgcc attgcagtca tcatcaatgc tgttgcatat
     3961 ttctgttgca ccgggtttga taccgctatt tgcgtcatca caatccagac tattcgccac
     4021 ataccctaac ggtgcgctac aagctgtggt agttgatcct gcatttccat aaccatctgc
     4081 atccacatca gcataatagg tgaaaggtcc aacaaacgat gtagttccag atataaatcc
     4141 gtctaaaagg tgttgttgac cagcagaatt gtcggcgttc catccataaa tcctgaagat
     4201 cagtgtactt gtagttgtaa aatcaggcac atcccaatta tacacttcac ctacctcaca
     4261 aggattcgca tttgtggcga agtccgaacc tgcatctacc caggtaacac caccatctat
     4321 actataagcc attcgattca gctgtggacc tgctgcatct ctgcggatgg taaattgaaa
     4381 gctagttacg gtcatagaac ggcaagactc tggtatcaaa acaatctgca tagcagaaaa
     4441 accggtgtta aaaggtccgt cagaaagttc ccagttagta gaaccaagcc ctgaagaaca
     4501 ccatacttca gagtgtatag gtccgttcac ttcgtctaaa agatcaaaaa tcagacctgc
     4561 ggcaaccgcc tcggccacgc catcctcatc gcccttatag tcaaataaaa tttgcgcatg
     4621 tagctgcata gaaagcgtca tggccacaat tgtagccagg ataaagtggg tttgcgtagt
     4681 cattttgtga taatatttaa actcaaatgt aaaaaaaaat caattttgat atggccggac
     4741 ggcccaattg gtggatactt ggaggatagg aggtgacaaa acgaaggaaa acatagtatc
     4801 aagcattaca cctgccggtt ttgttgaaaa ttgagcaata tcctgtctgt tgttggattt
     4861 taatgtggtt atgaatgttg tctacttgca gtatgagggt aaaaacggat taatcaataa
     4921 aattttttaa aaaaaatact tagaaatatg ttgcgataag caccgaaaag aggtatcttt
     4981 cgcaaaaaat atctcaatga tttctaaaag caactacagt cgattagagc attggattga
     5041 ggaatgtcaa tccaaaggca agctggcttt taatttggcc gagttaagac aacgtttcct
     5101 tgctgataca gaaatcgctc taaagcgggg attagacagg ttatcggaaa aagaaaaagt
     5161 agtttccata tttaagggat attatatcat tattccgccg caatatttat caaaagggat
     5221 tctaccggta acaatgttta ttgacggcct aatgaaattc ttagatagaa aatactatgt
     5281 aggtcttttg aatgcagcag ctttacatgg cgcatcgcat caacaaccac aggaatattt
     5341 tgttgtgaca gaatatccaa taatgagacc gacccaaaag aaaggcctta aaataaacta
     5401 catcagtaga agacaacttc cacctgaatc acttacagaa aaaatgaaaa ctgaaacagg
     5461 gtatattacc gtttcaaatc cattgttaac tgcgattgat ctgattacct acgagaaaaa
     5521 agttggtgga cttaataggg cttcaaccgt aattagcgag ttgtttgaaa caataaaacg
     5581 taatgatata aatgaggcat taataaatta tgcgagtgtt tcctcccttc aacgattagg
     5641 ttttgtgtta gatgaagtta taaacaaaaa agaaattgca gacaaaatac tttcactctg
     5701 caaaaaaacg ggagtgaatt tttatttgat tccattgaag gcgtcgggca acaattataa
     5761 agagcattta aatgagaaat ggaaattgat gattaataca aaaattgaaa ccgattttta
     5821 attaattctg tgatattgtt gaacaacaat aatgattgaa ataaattgac acatgatacc
     5881 acaagcatat ataacagaat ggagcaacaa tgtaccttgg cagacaaatg aacaggtaga
     5941 gcaagacctt gtgatttgca gagcattagt tgaaatattt aaggacgatt ttctttcgga
     6001 tagtttggca ttcagaggag gcactgcctt acataaatta tatctacaac cacagccaag
     6061 atattcggaa gatatagatt tggttcaaat tcaagctggt tctattggga aggtgattga
     6121 ccacatccga aaagcattat cctttttagg aaaagcgaaa gttgaaaccg gagaaactat
     6181 ggcaacaatg aaatttagat ttgattcaga gtttccgcca atagtttctt tacgcttaaa
     6241 aattgaaaca aattgcagag aacattttac ggtattgggt tgggagaaaa agttttttga
     6301 agtaaaatca tcgtggttca ccggtgaatg taatttaatt acctatcggt tggaagaact
     6361 tttgggaaca aaactcagag cattgtatca aagaagaaaa ggcagggatt tatacgattt
     6421 atggaaagca ttcacagcct caaatctcaa tccggacctt ttgataaaat gttacaaaga
     6481 atacatgatt ttttctttag aaaagaaaat acctacaaaa aaagaattcc taatgaatct
     6541 ggaagaaaaa aaagttgatg ctgatttttt aggcgacaca gctgcattgc tgaggccgga
     6601 tgaaaaatat aatcatgacg aggcattcag aattataatt gacacactga ttgaacgaat
     6661 gtaaagtttg tggaaatact tttctaaccg agtccgaatt ttaatacatt tgcaataagc
     6721 aagtttcgtg caacaaatcc agtttagttg gtattgtgtt atgcataaaa ggtttgtttt
     6781 attactacat ccatcacaaa tagaccaaaa gttattgtta aactcataaa ataataatca
     6841 accttaataa tgacaaaacc aaccttaccc actattccat tcaaaacccc caaatcattc
     6901 gaaacctggc ttgaaaaaca gcatgataag tcaaccggaa tt
//
LOCUS       DAORKW010000146         6930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_360_length_6930_cov_6.125527, whole genome shotgun sequence.
ACCESSION   DAORKW010000146 DAORKW010000000
VERSION     DAORKW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_360_length_6930_cov_6.125527"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="PLB46_07295"
     CDS             complement(<1..1436)
                     /locus_tag="PLB46_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76360.1"
                     /translation="MKASTELFDLIKSMSRSEIVSFKIACGADKQIYLQLFDAICEQT
                     VYNEAALKKLLYKELKTAKFAVLKKYLFDRLDKFLSARYDEQDAYFIMMKHYKMAQIY
                     YDRQLLDLCKKELQKAEMLAESNHCYAEQLLIYKLKEKLIRGLQTPADFEKLHNEFML
                     KESHAFSLYSNLSNYKHLDSEFVIALNKLNNISSDAALNEIKRIWDYPLLSDENNAHS
                     LLAKYFFYTIKASCANLLGKLEEGLIYREKCVQLLSQNSFEGADYNRLYIVSTHNYLK
                     NLCTLKMWEKFRLAFNQMEQFIQLKYDGIDQRSRMFLFAIIIFNKPDEIILSGNNSDF
                     KAYLKFFESEMKIHINSLDPSYVAFLHVSVIYNCIRFAIPEKALDLINRMNALGLDKK
                     LDKLYNKVFRLFVLMVHFDLKNYRLLRNLAEAHLRYLQRNGKPTRFEKSLTRFFHGIS
                     FDKDALIQLHGELTEIQHHNEEKFQFEY"
     gene            complement(2037..3614)
                     /locus_tag="PLB46_07300"
     CDS             complement(2037..3614)
                     /locus_tag="PLB46_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRF76361.1"
                     /translation="MRLNLLLLPICCTLLFLSDLRAQIYQQWDYNFSGPESRSDNGQD
                     IVIDNENNVIAVGIEDDADAFVGKWTNNGTLLWTATHNGSANLTDAFYAVAIDDENNI
                     YAAGISHEATSSWDIVTNKYNADGVLQWSKLKNGSSNIEENATDIAVDPFGNIYTAGY
                     IDQIDEGQDYCVIKYDTDGNELWMQTWGGPVGGSDVVYEMAIDFEGNVLLNGRIGSGG
                     DSLDDFATIKYNPEGVLQWVKTYHQIYGETGFTIDVDGEGNVYAGGWAADGLSMGESL
                     IIKYDPDGNTLWEQRYHNGLGGVSEAWVLRTYEDVVYMAGASIDWDLTGQDFMVLKYD
                     ADGNLLWDVAYDNGLNIADYLTSMTIDNAGNIVTSGLISDFTSGDILTVKFNPDGEVL
                     WAMTYNGDANSYDEAWGVTTDNDGNVFVTGFDINESVYSDLVVIKYGEQQVENIQTQV
                     NDLQLYPIPSSDVLFVDASIAFGDIVLMNTLGQIVLQQSINYTSQGIDVSAIPAGSYI
                     VKCISGEKTFVGKCVIK"
     gene            3886..5382
                     /locus_tag="PLB46_07305"
     CDS             3886..5382
                     /locus_tag="PLB46_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76362.1"
                     /translation="MSRKEKIHFRVHTKRLSSSDSKKYLNLFDLIAGQSTYQENAIRR
                     QIGMTGKNAQFAVLKNYLYNAIVTHLTNFHTENNEIYQAREYLRTANMLYDKQLIRQS
                     LKYIQKAKVIAEKNHRYYDLMGILFQEELINKNSPDIKQYSETYDMHFKKELGVIEQY
                     LNTRQYIYIDCQLLNAIRTTDNFSHNKSQEKILHILQHPMLRDETQAISLFSQIYFNT
                     INGICYHILADDEKSYNYRKKLVDVMERDLLNTSGYFGNYIGSLHNLTVTEIKLKKFA
                     EAELHLAKLEAFQQEYSKQLSERSKVLIKSSHALNTLSLLGYCGDKTTTMSFIATTDT
                     FITRDIKKMDYRYLIHIYYSIAYTAFKFSEYAIAIKYLTKLNVESSSGKLDRYLQRGY
                     RLLLLMVHLEMGNFRLLQYMVINTYRYLLKIGHLTKFDKAIISFIRSNSMDKKALLQF
                     QKQLQAAKADKFEAQEFSHLDVISWVESKLEKKPFIEVYRKNGSGYRP"
     gene            complement(5479..>6930)
                     /locus_tag="PLB46_07310"
     CDS             complement(5479..>6930)
                     /locus_tag="PLB46_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF76363.1"
                     /translation="GAKSGMGTLPILDFGTEAQKQKYLPGLASGKLHASYCLTEPGSG
                     SDALAAKTTAVLSADGKNYLLNGQKMWITNAGFADIFTVFAKIDGEKFTAFIVEKGAP
                     GFTIGVEEKKMGIKGSSTCQIFFENTPVPVENVLGEIGKGHLIAFNILNIGRYKLCVG
                     VLGGAKALIDISVKYANERIQFNVPISSFGAIKYKLSEQAVRTFAALSATYRTSDYID
                     KKEKELLAQGVSFEKALLGAAEEYAIECAMLKVIGSEVLDYVVDECVQIHGGNGYSEE
                     YPAARSYRDARINRIFEGTNEINRLLTVDMLLKRVMKGQLDMMGPAMAVQKELMAIPD
                     FGSGDEGLFAAEGKALKNMKKAFFMVAGAAVQKLMMKLKDEQEILMCAADMMNEIYLA
                     ESMLLRVKKLTDTKGEAATAIYRDIVNVFYTDSIDRLNKAGKTAIASFATGDELRMML
                     LGLKRFTKYEIHNTTEARRRIADKMIAEGVYCY"
BASE COUNT         2284 a         1367 c         1056 g         2223 t
ORIGIN      
        1 atatattcaa attgaaattt ttcttcgtta tggtgctgta tttccgtcaa ttcaccatgt
       61 aattgtataa gcgcatcttt atcaaatgat attccatgaa aaaaccgggt caaacttttt
      121 tcgaagcgtg ttggcttacc atttcgttga agatatcgca gatgcgcttc agcaaggttc
      181 cgaagcaaac gatagttctt caaatcaaaa tgcaccatca aaacaaacaa cctgaaaacc
      241 ttattatata acttatctaa ctttttatct aaacctaatg cattcatacg gtttatcaaa
      301 tcaagtgctt tttcaggaat agcgaaccgg atacaattat aaataactga aacatgtaaa
      361 aatgcaacat aagaagggtc aaggctattt atgtgaattt tcatttcaga ttcaaaaaat
      421 ttcaaatatg ctttaaagtc tgaattgtta ccagacaaaa ttatttcgtc aggtttatta
      481 aagatgataa tggcaaacaa aaacatgcga cttcgctggt caattccatc atatttgagt
      541 tgaataaatt gttccatttg attgaatgcc aggcggaact tctcccacat ctttagtgtg
      601 cataaatttt ttaaataatt atgcgttgaa acaatgtata acctgttata atcagcacct
      661 tcaaatgaat tttgcgacaa aagttggaca catttttctc tatatattaa cccctcctcc
      721 agtttgccaa gcaggttagc acaagaggct ttaatagtat aaaaaaaata tttggccaat
      781 aacgaatgtg cattattttc atcagacaat aatgggtagt cccaaatccg ttttatctcg
      841 ttaagggcag catcggaact aatattatta agtttattta aggctatcac aaattccgaa
      901 tcgaggtgtt tatagttaga aagatttgaa tacaggctaa atgcatgtga ttcttttaac
      961 atgaattcgt tatgcaattt ttcaaaatcg gctggggttt gtaaccctcg aattagtttt
     1021 tctttcagct tataaatcag cagttgctca gcataacaat gatttgattc agcgagcatt
     1081 tcggcttttt gcaattcctt tttacataaa tcaagcaact gtcggtcgta gtaaatttga
     1141 gccatcttat aatgtttcat cattataaag taggcatcct gttcatcgta ccgggcggac
     1201 aaaaatttat ccaggcggtc gaatagatat tttttgagga cggcaaactt agctgttttc
     1261 agttctttat ataacagctt tttcaaagca gcttcattat aaacggtttg ctcgcatata
     1321 gcatcaaata gctgcaaata aatttgttta tctgcgccgc aagcaatttt aaaactgact
     1381 atctccgacc tagacataga tttaatgagg tcgaacaact cggtactggc tttcaattga
     1441 attagtattt tagaatatca accgtttata aagtcaaaag taaacgaata tttcatttct
     1501 tcaaatgact taactaaaca attaaggttg caggccggtt ttccatggta ctattgctaa
     1561 ccggttgaaa atattaccgc ccatcgagta aaatcatttt taacctatta taaatattta
     1621 gctaaataca attttaggtt atacaccagg taaacttagt gcacttttca atttaaactg
     1681 aaaattagcc aactacctgt gggcgactga cgtgtattaa gttgttcaaa acaaagggag
     1741 cggaaaatcg tcataatttt ccgctcccac ataaacacta attacactac aacctggtat
     1801 aaccgtcatt ataccggggt ttcttaacta accattcatt atataaccga tttttgtatt
     1861 ggcaatatta aatcgcctgt tatttcttta atcactcctg ccgttgtatt gctaaaaaaa
     1921 aatgtcatat ttatactaat ttgagctagt gaaataaaat caatggttgg catattttat
     1981 catattgcct tgattgccaa tagaattaaa gcataccaac gataataatt tataactcat
     2041 tttattacac atttacctac aaatgttttt tcgccggata tacattttac aatgtaggag
     2101 cctgccggaa tcgcactcac atcaataccc tgcgaagtat aatttatact ttgttgaagt
     2161 acaatttgtc ctaaagtgtt cattaaaaca atatctccaa atgcaataga tgcatcaaca
     2221 aaaagcacat ctgaagaagg tattggatac aactgaaggt cgttaacttg tgtttggata
     2281 ttttcaactt gctgttcacc gtatttaatt acaactaagt cgctgtatac actctcatta
     2341 atatcaaacc cggtaacgaa aacattacca tcattatcgg tggttacgcc ccaagcctca
     2401 tcgtaactat tagcatctcc attataggtc atggcccaaa gcacttcacc gtcaggatta
     2461 aattttacag ttaaaatatc gcctgatgta aaatcagaaa taaggccgct tgtcacaata
     2521 tttcctgcat tatcaattgt cattgatgtt aaataatcgg caatatttaa tccgttatca
     2581 taggctacat cccataataa attgccatca gcatcgtact ttaaaaccat aaaatcctga
     2641 ccggttaaat cccaatcaat gcttgcgcct gccatataca caacgtcctc atatgttctt
     2701 aatacccaag cttccgaaac acctcctaac ccgttatggt atcgctgttc ccaaagggtg
     2761 ttgccatccg ggtcatattt aataatgagc gactctccca ttgaaagacc atctgcagcc
     2821 caaccaccgg catatacatt tccttcacca tcaacatcaa tcgtaaatcc tgtttcgcca
     2881 taaatctggt gataggtttt tacccattgt aaaacacctt cggggttata tttaattgtt
     2941 gcaaaatcat ctaacgaatc gccaccactt ccgattctgc cattcaacaa cacatttcct
     3001 tcaaagtcga tggccatttc atacactaca tcactcccac caactggacc accccaagtt
     3061 tgcatccata attcattgcc atcggtatca tactttatta cacaataatc ctgtccctca
     3121 tcaatttggt caatataacc tgcagtataa atatttccaa aagggtcaac agcaatatct
     3181 gtcgcatttt cttcaatatt tgatgagcca ttttttaatt tactccattg caatacacca
     3241 tctgcattat atttatttgt aacaatatcc caagaggaag ttgcttcatg gctaattccg
     3301 gctgcgtaaa tattattttc atcatcgata gccactgcat aaaatgcatc agttaaattg
     3361 gctgaaccat tgtgcgttgc cgtccataat aaagtaccgt tatttgtcca tttacctaca
     3421 aatgcatctg catcatcttc aatgccaaca gcaataacat tgttttcgtt atcaataaca
     3481 atatcctgac cattgtcgct ccggctttca ggaccactaa aattgtaatc ccactgctga
     3541 taaatttggg cgcgcaaatc agataaaaat agtaatgtgc aacaaatagg gaggaggagc
     3601 aagtttaatc gcataagttg ttgtttgatt tagttggtta atagaaaaag gtgtggttac
     3661 cacacttgcc taattcagct caaaactaaa aagggactac ctgtaatagt gattaaattc
     3721 atttcattat taggctcaat ttgtatttct ttcaccaaaa atagaaggta atatattgtt
     3781 tatcagcact ttacattcta acattattag gtaactttat agttaaaatt tcaaatatca
     3841 atcagcatga caccatccaa cgatttgttt gatttaataa aatccatgag ccgaaaggaa
     3901 aagattcatt ttagagtgca tactaaaaga ttgagtagct ccgactccaa aaagtatctt
     3961 aacctatttg accttatagc aggccagtcc acttaccagg aaaatgcaat tcgcaggcaa
     4021 ataggaatga caggaaaaaa tgcccagttt gcagtattaa aaaactattt atataatgct
     4081 attgttaccc atctaacaaa ttttcatacc gaaaacaatg aaatttatca ggccagagaa
     4141 tatttaagaa cggctaatat gctttatgat aagcagttaa tacgccaatc gcttaaatat
     4201 atacaaaaag caaaagtcat agcggaaaaa aatcatcgct attatgattt aatgggcatt
     4261 ttatttcagg aagagctcat taataaaaat tctccggata tcaagcaata tagcgaaacc
     4321 tatgatatgc actttaaaaa agagctaggg gtaattgaac aatatttgaa tactcgtcaa
     4381 tacatttata tagattgtca attactcaat gctatcagaa cgacggataa ttttagtcac
     4441 aacaagtccc aggaaaaaat tcttcatata ctacagcatc caatgttgcg tgatgaaaca
     4501 caagccatat ccttattttc acaaatatac tttaatacca taaatggtat ttgttatcat
     4561 attttggctg atgacgaaaa aagttataat tacagaaaaa aattagtaga tgttatggag
     4621 cgagacttgt taaatacttc cggatatttt ggcaattaca tcggttcact gcataacctg
     4681 actgtgacgg aaataaaact caaaaaattt gcagaagcag aattgcattt agctaaactg
     4741 gaagcatttc aacaggagta tagtaaacag ctttctgaac gtagcaaggt actcattaaa
     4801 agcagccatg cgttaaacac cttgtcgctg ttaggatatt gcggtgataa aacaaccaca
     4861 atgagtttta ttgcaactac tgacacattt attacaagag atataaaaaa aatggattac
     4921 agatatttga tacatattta ttattccata gcctataccg ctttcaaatt ttctgaatat
     4981 gcaattgcca ttaaatacct cactaaatta aacgtagaat ccagttccgg gaagttggac
     5041 cgttatttgc aaagaggata taggttattg ttattgatgg ttcacctgga aatgggcaat
     5101 ttcaggttgt tacaatatat ggtaattaac acttatcgtt atttactcaa aataggccat
     5161 ctcaccaaat ttgataaagc catcatcagt ttcatacgca gcaattcaat ggataaaaaa
     5221 gcattattgc agtttcaaaa gcaactacag gctgcgaaag ctgataaatt tgaagcacag
     5281 gaattctctc atttagatgt tatttcatgg gtagaaagca agttggaaaa aaaaccattt
     5341 atcgaagtat atcgaaaaaa cggatccggc tataggcctt aaacaatgtt aacctcgtaa
     5401 taagttttat caattaattc ataaaaaaag ccaacacaaa attggtgttg gcttttaatt
     5461 aattacctac ttacttcttc aataacaata cactccttct gcaatcattt tatcagcaat
     5521 tcttctgcgt gcttcggtag tattgtgaat ttcatatttc gtaaatcgtt ttaatccgag
     5581 taacatcata cgcaattcat cgccggtagc aaatgaagcg attgctgttt tacctgcttt
     5641 attgagcctg tcgatgctat cggtataaaa tacatttact atatcgcggt aaatggcagt
     5701 tgctgcctct cctttggtat cggtaagttt tttcacacgt aacaacattg attccgctaa
     5761 ataaatttca ttcatcatat cagcggcaca cattaatatt tcctgctcat cttttaattt
     5821 catcattaat ttttgaacag cagctcctgc caccataaaa aatgcttttt tcatgttttt
     5881 gagtgctttt ccttcagcag caaataagcc ttcatcacct gaaccaaaat ccggaatagc
     5941 cattaattct ttttgaacag ccattgccgg gcccatcatg tcgagctgac ctttcataac
     6001 acgtttcaat aacatatcaa cagtaagcag gcgattaatt tcgttggtgc cttcaaaaat
     6061 gcggttaata cgtgcatcac ggtaagagcg tgctgcaggg tattcctcac tatatccgtt
     6121 acctccgtga atttgtacac attcgtcaac tacataatcc aatacttctg agccgattac
     6181 tttcaacatt gcacattcta tcgcatattc ctcagcagca cctaataacg ccttttcaaa
     6241 actcacacct tgtgctaata attctttttc ttttttgtcg atataatcac tggtgcgata
     6301 agttgcgctt aatgcagcaa atgttctaac cgcctgttca cttaatttat atttaattgc
     6361 cccgaaagat gaaataggca cattaaattg aattctttcg ttagcatatt ttacagatat
     6421 atcaattagc gctttagcac cacctaatac accaacacat aatttataac ggccaatatt
     6481 taatatatta aatgcaataa ggtgaccttt acctatttca cctaacacat tttcaacagg
     6541 aacaggtgtg ttttcaaaaa atatctgaca agtagaagaa cctttaatac ccatcttttt
     6601 ttcttccaca ccaatggtaa agccgggtgc acctttttca acaataaatg cagtaaattt
     6661 ttcaccatca atttttgcga atacggtaaa aatatctgca aaaccggcat tggtaatcca
     6721 cattttttgt ccgttcagta aataattttt gccatctgca ctaagcaccg cagtggtttt
     6781 tgcagccaaa gcatcactac ctgaacccgg ttcggttaaa caataactgg catgtaattt
     6841 tcctgatgcc aagccgggta aatatttttg tttttgtgct tctgttccga aatctaaaat
     6901 tggcaatgta cccattccac ttttagctcc
//
LOCUS       DAORKW010000147         6887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_363_length_6887_cov_6.619438, whole genome shotgun sequence.
ACCESSION   DAORKW010000147 DAORKW010000000
VERSION     DAORKW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_363_length_6887_cov_6.619438"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /locus_tag="PLB46_07315"
     CDS             <1..1771
                     /locus_tag="PLB46_07315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF76364.1"
                     /translation="AITTELLNLFNAINFTTILSSWLILLIAIITTWWKKGKHLPEFK
                     LASPLLNIEKYIGLSLFVILFVTLITAVFAYPNNWDSMTYHLSRVMHWMQNENVKHYP
                     TRIDRQLTQPPLAEYHILHLFILGNRDSIANLVQWLFFAGSITSVALITAEFGGDRKA
                     QLLSAFVAATIPMAILQSTSTQNDLVVAFFVLTAILFGIKAWKQRFNTSYVYLFYITC
                     ALALLSKGTALIFLLPVVLVYAFFIVKVNRAKAIPSILKGIVIVLVIYGPFAYRNYQT
                     YNSPLGKSYELNNSIYTIKGTISNISKNTTMHLRSPVPAINNSLTNGVTAFNKAIGVD
                     VNAPNYNWAPSPDFVVGYFSTQEDSAGNFFQVILLVLVLVYIVMKFKSIPKHILLFTG
                     VIVCMYVLFNLLLKWQIWHARLHLTMFLAGAVLIAYWMNKLPSLATYSMVVVLSAFSL
                     VFLGYNQTRPLFGEHSIFKQPADKQYFAANEPLYAPFVKVNALLQENNCKTLGYISGG
                     DSWEYPFWALNKNNKSFSLYQLDTDNETKQLPQNTGSNIIIPDMIINIRGDEKKEEQI
                     SYMSNPYKLILLDGVFAVYKKDL"
     gene            complement(1766..2485)
                     /gene="recO"
                     /locus_tag="PLB46_07320"
     CDS             complement(1766..2485)
                     /gene="recO"
                     /locus_tag="PLB46_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRF76365.1"
                     /translation="MLHKTRAIVFKVTKYAESSVIIKAYTELFGVQSYIINGVRSAKG
                     KSKSALYQHGNLLDMVVYHKEQGNLMRISECGFAHIYEDLPFNIIKSSLLIFYLEILN
                     KIVKEHETNEALFDFLFEIFIELDQTEKSLGNHHIWFLLAITKYLGCYPTVSPDPYFD
                     LKEGMFIDFLPPHQQFLSEQLSNSFRAIIEPELKNYDQIKLTTTERKILLQQMLVYFR
                     LHIEDFGDLRSYEVLESLFKL"
     gene            complement(2547..3158)
                     /locus_tag="PLB46_07325"
     CDS             complement(2547..3158)
                     /locus_tag="PLB46_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76366.1"
                     /translation="MKQFFSKYFEYLLLGNTILMVFLKPMLPTYGERMSMILFGTLSF
                     YYLASGILVFLDKQRIGRIMRLIYLFGLWSVSITVMAVMARTLLLQMDKQLLIISVSS
                     GVGLMAYLFLYYRKLEADDKATLLIWIKPLVLRSILALCIAIAFLISSNYGIYSLFGT
                     HKNDPVYTEKAVNAYENPDDTAAVNDLNRYDALLDKKEEKPAE"
     gene            complement(3197..3649)
                     /gene="dtd"
                     /locus_tag="PLB46_07330"
     CDS             complement(3197..3649)
                     /gene="dtd"
                     /locus_tag="PLB46_07330"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRF76367.1"
                     /translation="MRVVIQRVLNAGVVIEQQTHANIGKGLMILLGVEENDSIEDITW
                     LTQKIAQLRIFEDANGLMNISVQDIDGEILVVSQFTLFASTKKGNRPSFIRSAKPDIA
                     IPLYEQFVASLAQLINKPVVTGVFGADMQVQLINDGPVTIIIDSKAKE"
     gene            complement(3651..4589)
                     /gene="rsgA"
                     /locus_tag="PLB46_07335"
     CDS             complement(3651..4589)
                     /gene="rsgA"
                     /locus_tag="PLB46_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRF76368.1"
                     /translation="MRGTVVQSTGNFYDVLLADGSIIICRLKGKFRLDNKKQTNPIAV
                     GDFVMVEEGPGETDEQKNYIINEILPRINYIVRSDIHKAAYKHVIASNIDQTVVIASL
                     KQPRTAFGFIDRVLITSEVYGIPAKVIFNKHDLYEEKDDELLQELMYSYNEAGYESFS
                     TSVLNKENLEQLHQVFENKTSLIIGLSGVGKSSLVNSIFPHLNLKTGAISAYTEKGLH
                     TTTFAHMYRINEHSFIIDTPGIKEFGLVDIDEYEVGHYFPEIRKEMQNCKFNNCLHIN
                     EAGCAIKQLLEEGKFSPSRYTSYLTFYEEIKGAKKW"
     gene            complement(4625..5392)
                     /locus_tag="PLB46_07340"
     CDS             complement(4625..5392)
                     /locus_tag="PLB46_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HRF76369.1"
                     /translation="MPLSETMRNQVYQNIAADHSKGLKKFAVLIDPDEVDAKALNQLV
                     TLGNENHVDYFFVGGSLVIGDYLDTTIKTLKQQTNIPVVIFPGSIQQVNPNADALLFL
                     SLISGRNPELLIGNQVLAAPYVKKSGIEAISTGYILIDGGAPTTVSYISNTNPIPSDK
                     PEIAACTAMAGELLGMKLIYMDAGSGAKRAIPAEVIAAVHKHCDLPIIVGGGIRTPEK
                     AYETCNAGADIVVVGNAIEKDTSLIKEISFAVHSVTV"
     gene            5998..6591
                     /locus_tag="PLB46_07345"
     CDS             5998..6591
                     /locus_tag="PLB46_07345"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRF76370.1"
                     /translation="MNTDFKDAMSKRTDEDLIKIVFVDRDAYQPMAIEAAEEEIKKRN
                     IDMTKIEQVKVDLKAKIEEQKEIDSKKLSALTRFIHFIVDTIAFIIFTMIFTFVIGLF
                     INPTDQNLKIILRYLMFATGFIVYYVFMETKYQKTIGKFITNTKVVNNNGTKPELGDI
                     VRRTFCRLIPFDQISFLFTPNGFHDRLSDTTVIKDKN"
BASE COUNT         2257 a         1262 c         1194 g         2174 t
ORIGIN      
        1 cgctattact actgaactct taaatttatt taatgccatc aattttacta ctatactaag
       61 tagttggttg attttgttaa ttgccatcat tactacatgg tggaaaaaag ggaaacacct
      121 gcctgagttt aaactcgctt ctcctttact gaacattgaa aaatatattg gtttatcatt
      181 atttgtaata ttatttgtta cattaattac ggctgttttt gcttatccca ataattggga
      241 tagtatgact taccatttat cgcgggtaat gcattggatg caaaatgaaa atgtaaaaca
      301 ttatccaacc cgcatcgacc ggcaattgac tcaacctccg cttgcggaat accatattct
      361 tcacttattt attttaggca atagagatag tattgcgaat ttagttcaat ggttgttttt
      421 tgcagggtca ataacaagtg ttgcattaat tactgcggag tttggtgggg acaggaaagc
      481 acaattgtta tctgcatttg ttgctgcaac catacccatg gctatattac aaagcaccag
      541 cacacaaaac gatttggtag tcgcattttt tgtattgact gccatattgt ttggaattaa
      601 agcctggaaa caacggttta ataccagtta cgtttatttg ttttatataa cctgtgcatt
      661 ggccttattg tcaaaaggaa cggcactcat atttttactt cctgtggtat tggtgtatgc
      721 attttttatt gtgaaagtaa acagggcaaa agcaattcca agtatcctga aaggaatagt
      781 tattgtgttg gtaatatatg gaccttttgc atatagaaat tatcaaacat ataacagccc
      841 attgggtaaa tcgtatgaac tcaataattc gatatacacc ataaaaggaa cgatatccaa
      901 tatttccaaa aatactacca tgcatttgcg gtctcccgtt cccgcaatta ataatagttt
      961 aactaatggg gttaccgcat ttaataaagc aattggtgta gatgtaaatg cgccaaatta
     1021 taattgggcg ccgagtccgg attttgtggt tggctatttt tctacacagg aagattcggc
     1081 aggaaacttt tttcaggtaa tattattggt gttagtattg gtttatattg ttatgaaatt
     1141 taaatcgata cccaaacata tattattatt tacaggtgtt attgtttgca tgtatgtatt
     1201 atttaatttg ttattaaaat ggcaaatctg gcatgcgcgt ttacatctta ctatgttttt
     1261 agccggcgct gtgttaattg cttattggat gaataaatta ccatcattgg caacttatag
     1321 tatggtagtt gttttgagtg ctttttcact tgtttttctt ggttacaacc aaacccgccc
     1381 gttgtttggc gaacacagta tatttaaaca accggcagat aaacaatatt ttgcagctaa
     1441 tgaaccattg tatgcaccgt ttgtaaaagt aaatgccttg ttacaagaaa ataattgtaa
     1501 aaccctgggt tatatttccg gcggcgatag ttgggaatat ccattttggg cgttaaataa
     1561 aaacaataaa tcgttttcac tgtatcaact cgataccgat aatgagacaa aacaactgcc
     1621 acaaaatacc ggcagtaata tcattatccc tgatatgatt attaatataa gaggcgatga
     1681 aaagaaagag gagcaaatat cctatatgag taacccctac aaattgattt tgttggatgg
     1741 tgtatttgcc gtttataaaa aggatttata atttaaataa ggattccaat acttcgtaag
     1801 agcgcaaatc accaaaatct tcaatatgca aacggaagta taccagcatt tgttgtaata
     1861 aaatttttcg ttcagtggtg gtgagtttaa tctggtcgta attttttaat tcaggttcaa
     1921 taatggctcg aaatgagttg gataattgtt cgcttaaaaa ttgttggtgc gggggtagga
     1981 aatcaataaa cattccctcc tttaaatcga aataagggtc aggcgaaaca gttgggtagc
     2041 agcctaaata tttagtaata gccaataaaa accaaatatg atgattgcct aaagattttt
     2101 cggtttgatc taattcaata aatatttcaa ataaaaaatc aaataacgct tcattggttt
     2161 cgtgttcttt tacaatctta tttaaaattt ccagataaaa tatcagcaaa gaacttttta
     2221 taatattaaa aggcaaatct tcataaatat gcgcaaatcc acattcgctg atgcgcatta
     2281 agttaccctg ctctttgtgg tagactacca tatcgagcaa attgccatgc tgatataagg
     2341 cagatttcga tttgcctttt gcagaacgaa cgccattaat gatatacgat tgaacaccaa
     2401 ataattcggt ataggccttt atgattacac tggactcagc atatttcgtc accttaaata
     2461 ctattgccct tgttttgtgt aacatagtgt aaaagtaaac aacacaatgg tatcggtatt
     2521 aaaataagtg taagaatgaa gctaaattat tcagcaggct tttcttcttt tttatccaaa
     2581 agagcatcgt atctattcaa atcgttaact gcagcagtgt catccggatt ttcataggca
     2641 ttaacagcct tttcagtgta aaccgggtcg tttttgtggg tgccgaataa actataaatg
     2701 ccataattac tgctaattaa aaaagcaata gcaatgcaca aagccaatat agaacgcaat
     2761 accaaaggtt ttatccaaat gagcaaagta gctttatcat ccgcttcgag tttgcggtaa
     2821 tataaaaaca aataagccat taaacccaca cctgatgaaa cagaaataat gagtaactgc
     2881 ttatccattt gtaataacaa tgttctcgcc attacagcca ttacggtaat agataccgac
     2941 cacaagccaa ataaatatat taaccgcatg atccggccaa tacgttgttt atccaaaaaa
     3001 actaaaatac ctgaggcgag ataataaaaa gataaggtgc caaataaaat catactcatg
     3061 cgctctccat aagttggtag cattggtttt agaaacacca ttaaaatggt attacctaat
     3121 aataaatact caaaatattt ggaaaaaaat tgtttcaata tgctaaatct ggtgtaaaat
     3181 tataaaataa gcagcactat tcttttgctt tgctgtcaat aataatggta accggtccat
     3241 cattaattaa ctgcacctgc atatctgcac caaatacgcc tgttacaacc ggtttattta
     3301 tgagttgcgc taatgatgca acaaattgtt catacaacgg aatcgcaata tccggtttcg
     3361 cactacgaat aaatgaaggg cgattgcctt ttttggtgga ggcaaataaa gtaaactgac
     3421 tcaccaccaa tatttcccca tcaatatcct gaacgctgat attcattaat ccattggcat
     3481 cttcaaaaat gcgcagttgg gcaatttttt gagtcaacca ggtaatgtct tctatggaat
     3541 cattttcttc tacccccaat aaaatcatca atcctttgcc gatgttggca tgtgtttgtt
     3601 gttcaataac tacaccggca ttaagcacac gttgaattac tactctcata ttaccacttt
     3661 tttgcgccct ttatttcttc ataaaaagtt aaataactgg tatatcgcga aggcgaaaat
     3721 tttccttcct ccagtaattg ttttatcgca caacccgctt cattaatatg taaacaatta
     3781 ttgaatttac aattttgcat ttcttttctt atttccggaa aataatgtcc cacttcatat
     3841 tcatcaatat ctaccaaccc gaattcttta attcccggtg tgtcgataat aaatgaatgt
     3901 tcgttaatgc gatacatatg agcaaatgta gtggtgtgta atcctttttc agtgtaagca
     3961 gaaatagcac ccgtttttaa atttaaatgc ggaaatatac tgttaactaa cgatgatttc
     4021 ccaaccccgg ataaaccaat aattaacgat gttttatttt cgaacacctg atgtaattgt
     4081 tccagatttt ctttatttaa aaccgaagta gaaaaacttt cgtaacccgc ttcgttataa
     4141 ctatacatta attcctgcag cagctcatca tctttttcct cgtataaatc atgtttatta
     4201 aaaatcactt ttgcaggaat gccatacact tcgctggtaa ttaaaaccct gtctataaat
     4261 ccaaaagccg tgcgtggttg tttaagcgaa gcaattacta cagtttggtc gatattactt
     4321 gcaattacat gtttgtaagc cgctttatga atatcggaac gaacaatata attaatgcgc
     4381 ggaagaattt cgttgataat ataatttttt tgttcatctg tttcgccggg tccttcttcc
     4441 accataacaa aatcaccaac agcaatcgga ttggtttgtt ttttattatc gaggcgaaat
     4501 tttcctttca aacggcaaat aatgatgctg ccatcagcta ataacacatc ataaaaattt
     4561 ccggtacttt gaactacggt tccgcgcatg gtgtaaagtt aaaattcagg aagtaaatac
     4621 gcgattaaac agtaacagaa tgcactgcaa acgaaatttc cttaatcagt gaagtatctt
     4681 tttcaatggc attgcccacc accacaatat cggcgcccgc gttgcaggtt tcgtatgctt
     4741 tttcaggagt tctgatccca ccgccaacta taattggcaa atcgcaatgt ttgtgcacag
     4801 cagcaatcac ttcggcagga atagcccttt tggcaccgct gccggcgtcc atataaatca
     4861 gtttcatacc caataattca ccggccatgg cagtgcaggc cgcaatttcg ggcttatcgg
     4921 aaggaattgg atttgtattc gaaatataac tcaccgttgt cggcgcgcca ccgtcaatca
     4981 ggatataacc cgttgaaata gcctcaatac ccgatttttt cacgtaagga gccgccaaca
     5041 cctggttgcc aatgagcaat tcggggttac gtccgcttat aagcgataaa aacagcagtg
     5101 catcagcatt tgggttcacc tgctgaatac tacccggaaa aatcaccacc ggaatattcg
     5161 tctgctgctt cagggtttta atggtcgtgt ccaggtaatc gccaataacc aggctgccac
     5221 ccacaaaaaa gtagtcgaca tggttctcat ttcccagtgt taccaattgg ttcaacgcct
     5281 ttgcatccac ctcatccggg tcgattaaaa cggcaaactt tttcagtccc ttactgtgat
     5341 cggctgctat attttgatat acctgattgc gcattgtttc ggataaaggc aaaaataggg
     5401 ttttttgctg acaggaaagc aggtgcccga ccaataaacc gtttcttttt tttggagcag
     5461 gaacatttct gcacaccaac aaaatcggca cgtgctttcc actgcaactc cgcttccgct
     5521 gaaaaagcgg aatgcggggt ttccgtttca atcacggcta actgtgcata ttatcgtaac
     5581 tatcacaaaa ttattgcgta tagtttaaag cagttttgtg acatggtaaa actatcaaaa
     5641 acgcaaaaga ttaaatccgt aaaataatag atataagtct gtaacatatt ggatgttatt
     5701 aaaagtgtga catagtctat agccagcctt tctaaaccct ttacagccct taaaatcatt
     5761 ttcaataaca gttattcacg caacaaattt ttgaaatgca acaattgcca agatttcttt
     5821 gtgcaacttt tcccaaataa tatatttaaa aaacgcaatt tttatatttg ataattaatg
     5881 cattgtggtt tttttttaag gactttcgga tatagtttgt tttgaaaaaa taatcttata
     5941 ggctatcttg cacgaccgaa aagcgaaaac attaagtcaa acataaaatg acaaacaatg
     6001 aatacagact ttaaagacgc aatgtcaaaa cgaactgacg aggatcttat taaaatagtc
     6061 tttgttgaca gagatgccta tcaacctatg gcaattgaag ctgctgagga agaaatcaaa
     6121 aaacgtaaca ttgacatgac aaaaattgag caagtaaaag tcgacctgaa agcaaaaatt
     6181 gaggaacaaa aggagatcga ctctaaaaag ctgagtgcat tgactaggtt tatacacttc
     6241 attgttgaca caattgcgtt tataattttt acaatgattt ttactttcgt aattggactt
     6301 tttataaatc cgacagacca aaatttaaag ataattttgc gttatttaat gtttgcaaca
     6361 ggcttcattg tatattatgt ttttatggaa actaagtatc aaaagacaat tggaaagttt
     6421 ataactaata caaaagttgt aaataataat gggacaaagc cagagcttgg agacattgtg
     6481 agaagaacgt tttgccgtct tatcccattt gaccaaattt catttctgtt tacaccaaat
     6541 ggttttcacg acagactttc tgacacaaca gtaattaaag ataaaaacta acaaaaacaa
     6601 agaaacacag cttataacac gttttggcaa aagtgacggt tctgtgcccc gcagaaaaaa
     6661 ttcaaatcga agacatttat tcttacaaca ttttagccaa attgcagata ttgcatttga
     6721 cgaaaccgga gttgctaaaa taaaagactt aatgccggga atatatttca cccagctaac
     6781 tacaaaaacc gcaacaattg taaaaaaatg gatttaggag taaggtgaaa acgaaaaaag
     6841 agagatggtg gtttgggtga aggagtgagt atggatattt gaattgc
//
LOCUS       DAORKW010000148         6819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_370_length_6819_cov_4.892815, whole genome shotgun sequence.
ACCESSION   DAORKW010000148 DAORKW010000000
VERSION     DAORKW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_370_length_6819_cov_4.892815"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..2087)
                     /locus_tag="PLB46_07350"
     CDS             complement(348..2087)
                     /locus_tag="PLB46_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76371.1"
                     /translation="MINHQNVFSQAGTLDPLFGVDGLVSTEVAPYDDWGTAIALQSDG
                     KIILAGQSGNGIDVDFSMARYTADGLLDATFGTGGVVITPVGIDDDRCWAINIMSDGK
                     ILLCGTANNGSNDDFAMVRYLENGTLDATFGTDGIVLFDFYGDNDYGYGVTTQLDNKI
                     VMVGSATVAYPHREFAIARFNNDGSVDNTFDFDGKVTTDMCDFTSEKATSVIVLSDNK
                     IVVGGNCFGSIGIARYNGDGSLDNSFSTDGHLVTNVGVDQTYPISIATQSDGKIVAAG
                     HEIEDAPYYTKMNFGLVRYNVDGNLDESFGDEGVVITNFDTLTGDVGLALAIQTDGKI
                     IVAGQSYTVCFGIGYFALARYNTNGTLDTTFGIGGLVNTDFGYSHDAAFAVAIQPDGK
                     IIAGGQSGPNDLARRYFGLARYLNDCFYATDKSIIQTSNILTAVATDLAYQWVDCEND
                     YLPVADGTNQTYTATQNGTYAVIFTDGACVDTSECVVVNTVDITSGSTSTAITVFPNP
                     FTETLSLTMNIDGTNNITGEIFLYNNLGELILTAKAINGKAVFNTAELFPGVYVVNCM
                     VGDVSRNLVVVKR"
     gene            complement(2278..4017)
                     /locus_tag="PLB46_07355"
     CDS             complement(2278..4017)
                     /locus_tag="PLB46_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76372.1"
                     /translation="MLTTLLILLLTFGTAYNQITSPSWYKGMLGYFDGVICTDSLLNV
                     YLSTHFYETSQFDYTGIDTTLLANGALLLVKMDQTGKMIWMNTINKYSDSPGFASSAA
                     HIVANNNNLFVGSVAYDTVTLKSGDPEFLVPSGTGFVSSFFAKYNNDGEPEWVKILSS
                     DSIIGISDIALDQFGNIFITGQFMGTADFNPGAGEFTINANETDNFLAKYDYLGNLLW
                     AYALSGEKGVGSIMLEIDKENNLLLCGNLFDKESADFDFGLGEQILDAERGYDLFIAK
                     YNNNGDYLWAFKAGNNSFNVNLKPTDFSIDEENNIICTGSISGPEPVDFDPSAVEFML
                     TPNYEHTNGFLAKYNPDGNFIFAYLWDPSSKIECNAVATSQTGNIFVFGNLLGSVDLD
                     PSPDEFIIETTGIGIPNDYDFYLMEFDAVGNFIDASQIITGLSNWSAAQKLVLDNADN
                     LIMVGFFQDSIDLDPTPGITTLYTIDTLPPFEYGNGFYIAKYGDAKTFVNSFESVEGI
                     ALYPNPALSTLNINYQGEAESVAIFDLTGKCLYWNNTPNQASVDVADFPSGMYCLRLC
                     QSGEWHSSKFIKL"
     gene            4225..6153
                     /locus_tag="PLB46_07360"
     CDS             4225..6153
                     /locus_tag="PLB46_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF76373.1"
                     /translation="MKAIILGIFTFVATVIPFAELSGQKQNNNWYFGTAGIDFNGPSP
                     VAITTSAMSTYEGCATVSDNFGNLLFYTDGVSVFNRENDVMPSGTGLFGDYSSSNSAV
                     IVPKPGDPNKYYIFTTECYYPLTGLHYTLVDMCLDGGLGGVVAGEKNVFLTGQTPEKV
                     TTALHANGKDVWVLTHGWNNNEFRVYLVTAAGVDPTPVVTAIGPVHGPDFFQSVGCMK
                     MNRDNNKIAVAVEVSGNRIELYDFDNATGIVSNEIIITTPHSAAGLGPGNTVYGVEFS
                     PNNNFIYSSSWFDDNIYQLDITSGDAATIEASATIIGTTSAASNVGALQLAPDNKIYA
                     ATQYKNKVGVINAPDFAGIACNYIDNGFNLEGETVYAGLPAFVQNNEISYQPEFEFTA
                     GCAGGTSAFSFTSDAVYDEVFWDFGDPTTTLDNSTAFDATYIYSDTGTYTVTLIFTIG
                     CFSDTAITDIVVNEGALYNLLPGDTTVCIEDASSIILFLTATLTDIEWSTGSTANEIE
                     VSADGIYFASGSDDCGILQTDSIVVDFIDCEIDTTTNENPTTCFIQFPNAFSPNGDGF
                     NEIFNPVANQLCLVENYQLQIYSRWGELIFSSNDINTGWNGIYKNIPQELGTYIYIAD
                     ATIGGMDMQQTGTVTLVR"
     gene            6320..>6819
                     /locus_tag="PLB46_07365"
     CDS             6320..>6819
                     /locus_tag="PLB46_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRF76374.1"
                     /translation="MKKLTPVLMLMCFAQYILAQTPTYTFPAETLQHEGTWLQWPHHY
                     TYGTTYRSRLDQTFIDMTAALVSSEKVFIVAYNNTEKNRIIDLLNTAGIPLGNIFFYI
                     IKTDDIWSRDNGPMYVYNEDNELVILDWKFNGWGNDTPYAKDDKVPQRIAEKTGVPRV
                     NLSAMV"
BASE COUNT         2201 a         1285 c         1125 g         2208 t
ORIGIN      
        1 acaatacaga ataatatata ccgtttcata agttggggtt tgattattac aattttaaat
       61 taaataacaa aattaaataa ttatcagtta agactgaata aacaaatatc atattcacct
      121 ataattgata agatttataa aggcatttct attgatatag aaaaaaggtt ttaagaataa
      181 actagttgga aaattgaaat agatatgtat ttaatcttga agaattaatt tcgattggat
      241 agattttaca tatacgacat gtaaacaata attaatttgg tgttgatttc aaaataaata
      301 tttgacacat ttaaatttac aattaaactt gatttaaagc gccaaaacta tctttttacc
      361 accaccaaat tcctactcac atccccaacc atacaattaa caacataaac tcccggaaat
      421 aattctgcag tattaaaaac agcttttccg ttaattgcct ttgcagttaa aattaattct
      481 cccaaattat tgtataaaaa tatttctccg gttatattat ttgttccatc aatattcatg
      541 gttagactca gcgtttcagt aaaggggttg ggaaacactg taattgctgt tgatgtgctg
      601 cctgaagtaa tatccaccgt attcaccacc acacattccg aagtatctac acatgcacca
      661 tctgtaaaaa taactgcata ggttccgttt tgtgtggctg tataggtttg attggtgcca
      721 tctgcaaccg gcaaataatc gttttcgcaa tctacccatt gataggcgag gtcggtggca
      781 acggcagtca aaatattgga tgtttgaata atcgatttat cagtcgcata aaaacaatca
      841 tttaaatatc gcgccaaacc aaaatatctg cgtgctaaat cgttgggacc actttgtccg
      901 cctgcaataa tttttccatc gggttgaatt gcaacagcaa aggcagcgtc gtgactgtat
      961 ccgaaatcgg tatttactaa tcctccaata ccgaatgttg tatctaatgt accattggta
     1021 ttatatcgtg ctaaagcaaa ataaccaatg ccaaagcata ctgtatagga ttgacctgca
     1081 acaataattt ttccgtctgt ttgaatggcg agtgctaaac caacatcacc tgttagtgta
     1141 tcaaaattag taattacaac tccctcatca ccaaacgatt catccaagtt gccatccaca
     1201 ttataacgca ccagcccaaa attcatttta gtataataag gtgcatcttc aatttcatgt
     1261 ccagctgcaa caatttttcc atcggactgt gtagcaattg aaattggata tgtttgatct
     1321 acacctacgt tagttaccag atgtccgtca gtactaaaac tattatccaa cgaaccatca
     1381 ccattatatc gcgctatacc tatagaacca aaacaatttc caccaactac aattttatta
     1441 tctgataaaa caattaccga cgttgctttt tctgaagtga aatcgcacat atcagtagta
     1501 acttttccat caaaatcaaa agtgttgtct acagagccat cattattaaa acgtgctata
     1561 gcaaattccc gatgcggata agctacagtt gctgaaccta ccataacaat tttattatcc
     1621 aactgcgttg ttacaccgta accataatca ttgtcaccat aaaaatcaaa taaaacaatt
     1681 ccatcagtgc caaaggtagc atctagtgtg ccattctcaa gataacgcac cattgcaaaa
     1741 tcgtcattac taccgttatt agctgtgccg cataaaagaa tttttccgtc gctcatgata
     1801 ttgattgccc aacatctgtc atcatcaata cccaccggag taattaccac gccaccggtt
     1861 ccaaaagttg catccagcaa tccgtcagca gtataccgcg ccatcgaaaa gtctacgtct
     1921 atcccatttc cactttgacc ggccaaaata attttaccat cactttgaag cgcaatggca
     1981 gtgccccaat cgtcgtaggg agccacttct gtagaaacaa gtccatctac cccaaacaag
     2041 gggtctaagg ttcctgcctg actaaaaacg ttttggtggt ttatcatcag gagcagtaac
     2101 aatgttatga agctttggta gtgatgtttc attaatataa agttaccatt ttttactaat
     2161 atcatcatgt agcatttttc atttatgtgg tattgctcta ttttgttaca ccacaactcg
     2221 aacaaaatcc tcagatttaa ttaacacata atttagcatg ccgcagttat ctgaatttta
     2281 taacttaata aattttgagg aatgccactc tcctgactga cataaacgca aacaatacat
     2341 accacttgga aaatccgcga cgtctacact tgcctgattc ggagtgttat tccaatacaa
     2401 acattttcct gttaaatcaa aaattgcaac actttcagcc tcaccttggt aatttatgtt
     2461 cagggttgac agagcaggat ttggatacaa tgctattccc tcaacactct caaaagaatt
     2521 tacaaacgtt ttcgcatcgc catattttgc aatataaaaa ccattgccat actcaaaagg
     2581 aggtagtgta tcaatagtgt ataaagtagt gatacctgga gttggatcta aatcaattga
     2641 atcttgaaaa aacccaacca ttattaaatt atcagcgtta tccaatacaa gtttttgtgc
     2701 agctgaccaa tttgatagac cagtaattat ttgacttgca tcaataaaat tacccacagc
     2761 atcaaattcc ataagataaa aatcataatc atttggtatt ccaattccag ttgtttctat
     2821 aatgaattca tctggtgatg ggtcaaggtc aaccgatcct aataaatttc cgaaaacaaa
     2881 aatatttccg gtttgactag ttgcaactgc gttgcattca attttagaac tcggatccca
     2941 caaatacgca aaaataaaat tgccgtcagg attatactta gctagaaacc cattagtatg
     3001 ttcgtaattt ggtgtaagca taaattctac agctgaaggg tcaaaatcaa caggttccgg
     3061 tccagatata ctacctgtgc aaataatatt gttctcttca tcaattgaaa aatcggttgg
     3121 ctttaaattg acattaaatg aattatttcc ggctttaaaa gcccacaaat aatctccatt
     3181 attattgtat tttgcgataa ataaatcata tcctctttct gcatctaaaa tttgttcccc
     3241 aagcccaaaa tcaaaatctg cactttcttt atcaaataaa ttaccgcaca gcagcaaatt
     3301 attttctttg tcaatttcca acattatact tcctactcct ttttcacctg ataaggcata
     3361 agcccacaat aaatttccta aataatcata tttagctaaa aaattatctg tctcgtttgc
     3421 atttattgta aactcacctg cgccaggatt aaaatcggca gtccccataa attggcccgt
     3481 aataaatata ttaccaaatt ggtcaagtgc aatatccgat ataccaatta tgctatcaga
     3541 acttaatatt ttaacccact ccggttctcc atcattatta tattttgcaa aaaacgatga
     3601 gacaaatccc gtaccgcttg gcactaaaaa ttccgggtca ccggatttta gggtaactgt
     3661 atcatatgct acactaccaa caaataaatt attattgttg gcaacaatat gcgcagcaga
     3721 agatgcaaat ccgggtgaat cggaatattt atttatggta ttcatccaaa tcattttacc
     3781 ggtttgatcc atcttaacca atagtaaagc cccatttgca agaagtgtgg tatctatacc
     3841 ggtataatca aattggctag tttcataaaa atgtgttgat aaataaacat tcaatagtga
     3901 gtcagtacaa attacgccat caaaataacc caacattcct ttgtaccatg atggtgaagt
     3961 tatttgattg tatgctgttc caaatgttaa taataaaatc agcaatgttg tgagcatttt
     4021 cttcaatgtg gaatattttg aatataaata tagcactttt agccaacttc caatttatac
     4081 caattggtta tttctgccta aaacattgta tcaaatattt ggcatcagtt acttttaatg
     4141 ccacttcatt aaacttttca cctccatttt tgtttaagcc ttattaaaac aaaacctcct
     4201 taccataatc gtgttgaaat acatatgaaa gctatcatat taggcatttt tacattcgtt
     4261 gccactgtta ttccttttgc agaattaagt gggcaaaaac aaaataataa ttggtatttc
     4321 gggacagcgg gaatcgattt taatggcccc tctcctgttg ccattaccac cagcgccatg
     4381 agtacgtatg aaggctgtgc gaccgtttcg gataattttg gcaatttgtt gttttataca
     4441 gatggtgtta gtgtttttaa ccgtgaaaat gatgtgatgc cttctgggac cggtttattt
     4501 ggcgattact ccagcagtaa ttcagctgta atcgttccaa aaccgggtga ccccaataaa
     4561 tattatattt ttactactga atgttattac cctttaacag gattacatta caccctcgtt
     4621 gatatgtgtc tcgatggtgg attaggtggt gttgtagcag gtgaaaaaaa tgttttttta
     4681 acgggacaaa cacccgaaaa agtgactacc gctttacatg cgaacggcaa agatgtttgg
     4741 gtgcttactc atggttggaa taacaacgaa ttccgtgttt atttagtgac tgctgccggt
     4801 gttgatccta cacctgttgt tactgctatt ggtcctgttc atggtcccga ttttttccaa
     4861 agtgtgggat gcatgaaaat gaatcgcgac aataataaaa ttgcagttgc tgttgaagta
     4921 agtggaaata gaattgaatt atatgatttt gataatgcaa caggaattgt aagtaatgaa
     4981 ataataataa ctactcctca ttcagctgcc ggtttgggtc ccgggaatac tgtttacggt
     5041 gtggaatttt ctccaaataa taattttatt tattcctcaa gctggttcga tgataatatt
     5101 tatcaattgg atatcactag tggtgatgct gctactatag aagctagtgc aacaattatc
     5161 ggcactacat ctgcagcttc taatgttggt gcattacaat tggcacctga taataaaatt
     5221 tatgctgcaa cgcaatataa aaataaagtt ggtgtaatta atgcacctga ttttgccggc
     5281 atagcatgta attatattga taatggtttt aatttagaag gcgaaacggt gtatgcaggt
     5341 ttaccggcat ttgtacaaaa caatgaaatt tcttatcagc cggaatttga atttactgca
     5401 ggttgtgcag gtggaacatc agcattttcg tttacaagtg atgctgtgta tgatgaggtg
     5461 ttctgggatt tcggtgatcc aacaaccaca ttagacaatt ctactgcatt tgatgccacc
     5521 tacatttatt ccgatacagg aacttacacg gtaaccttaa tttttacaat cggttgtttt
     5581 agcgatactg ctattacaga tattgttgta aacgaaggag cattatataa tttgttgcct
     5641 ggtgatacaa cagtgtgtat agaagacgcc tcttccatta ttttattttt aactgctaca
     5701 ttaacagata ttgaatggag cacaggttca actgcgaatg aaattgaagt gtctgctgat
     5761 ggtatttatt ttgccagcgg tagcgacgat tgtggtattt tacaaacaga tagtattgta
     5821 gttgatttta tagattgcga aattgacacc accacgaatg aaaatcctac aacatgtttt
     5881 atacaatttc caaatgcatt ttctccgaat ggggatggat ttaacgaaat atttaatcct
     5941 gttgccaatc aactttgttt agttgaaaat tatcaattac aaatttattc ccgttggggt
     6001 gaattaattt tttcgagtaa tgatataaat accggatgga atggcattta caaaaacata
     6061 ccgcaggagc ttggcactta tatatatatt gctgatgcta ccataggtgg gatggatatg
     6121 caacaaaccg gaactgttac cttggtgaga taattgctaa agggtgtttt cactctcatt
     6181 ttatattacc ggcatttaat aaagctaaga ccctcgtttt tttgaatgag tgaaatatga
     6241 tatatttacc gggaatatgt taattgtttt tattcctaat aatatataca tccatttatt
     6301 ctccaaatcg ttttaggtta tgaaaaaact tactccagtt ttaatgctta tgtgttttgc
     6361 ccaatacatt ttagcacaaa cccctactta tacatttcct gctgaaactt tgcaacacga
     6421 aggcacctgg ttacaatggc cacatcatta tacttacggt acaacatatc gtagcagact
     6481 ggaccaaaca tttattgata tgactgcggc attggtgagc tctgaaaagg tttttattgt
     6541 agcctataac aatactgaaa aaaacaggat tatcgattta ttaaatacag caggaattcc
     6601 tttgggaaat atattttttt atattattaa aacagatgat atttggtcgc gcgacaatgg
     6661 acctatgtat gtatataatg aagataatga actggtaatt ttagattgga aatttaacgg
     6721 ttggggcaac gatacaccgt atgctaaaga tgataaagtg ccacaacgta ttgctgaaaa
     6781 aacaggtgtg ccccgtgtta atctttccgc aatggtatt
//
LOCUS       DAORKW010000149         6803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_373_length_6803_cov_5.083284, whole genome shotgun sequence.
ACCESSION   DAORKW010000149 DAORKW010000000
VERSION     DAORKW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_373_length_6803_cov_5.083284"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="PLB46_07370"
     CDS             <1..1887
                     /locus_tag="PLB46_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76375.1"
                     /translation="TATFTYPVGEWFEAEHIIDLDANTMEIYYDGVLVLSYDYPGSIG
                     AIDFYSAAANNKCYIDDVVLMEELPAEACAIPGAIFCDNIDTYTAGDAVGPYADWWST
                     WSGVEGGAEDGTVSDAYAYSGENSVLIPGTGTTDALLLLDNMASGIKRLEWQMFIPSG
                     KTAYYNIQESEVAGIAWNLELYFGDITEGQGEFTVPAAGPTFTYPVDEWFLVEHIVDL
                     DADNIKVYIDGVMVLDDVYTGSLGSIDFFSWSASNSYYLDDILYIEEDVVVAEPCDVP
                     GAVFCDNMDTYEEGALGPNADWWSTWSGVEGGAEDGLVSSDFAASAPNSALIPEGGVT
                     DVILKLGNLSSGKYNLYWNMYVPSGSHAYYNIQETEVPGVAWNAELYFGLATPGEGEI
                     TVPAELTFTYPPDAWFLIEHIIDMDANTIEIKLNGTVILSEVYEGNMGGVDFYSIDAL
                     NRYYIDDVALMPMDPTDLNTYYQDADSDTYGNADASITVAGDAPTGYVENNLDCDDTN
                     PDIYPGATEILNNKDDDCDELIDEGLINIETLAATGMAIQVYPVPANNNVTIELMSLM
                     SGEAATFEIYNTTGQVVYTEQLVLNTSTLVKQINIESLGSGIYMVKLTSGEHTVNGQI
                     IVQH"
     gene            complement(1992..3602)
                     /locus_tag="PLB46_07375"
     CDS             complement(1992..3602)
                     /locus_tag="PLB46_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76376.1"
                     /translation="MNLKPLQTSIPTQYAIAIVIALFAVVLVVFHPSYETSDDTMMNY
                     IAAGTLNGEPSQYLVFSNYLEGVFLKWLFVRFPGINWYPLFLYSIQLLAFVQITYVLN
                     KANSSRLFFGLYILIYAIFVVYVIINIQFTSTAFLAGIAGLLTLLHLIKNNTTKPLSI
                     LKMTGFLLLCVLVRWESYVGLLVITAPVLIYHFRKPVFSKKHFIYGVTLLFPLLLIYV
                     NLQCYASISSTHNYIQYQLATHKILDYDSEMSANTLQQVDLTTTDYKLLNTYFWADDK
                     VFTQEKIVQLAGLKSNLLDLPKGADVLVNYATSFLFYGALLVIIGFCFLFANAGQRKK
                     IIIYTVFIHLLLLLLASYLKLPLRLYLPLCFTVVMMILYEVSNRIQTTNLSLKSKVIG
                     LFLLVIAIMQTGINRNISNYHKELTAKNENAIKLVGLHPETLFIDIDYSDQLHRPSLF
                     APAPRIIGNNLIDGASFINTPTYHSLLAKNNIQNLTIDIVGKPAVFISNDAAFYSNYR
                     EFMREHYGVECGFVPYIGLEDAKLVIWR"
     gene            3766..4839
                     /locus_tag="PLB46_07380"
     CDS             3766..4839
                     /locus_tag="PLB46_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76377.1"
                     /translation="MKTSNKRTFFIQLAIFFIIGISGISYSYTQPVINYGIYPLVGES
                     YNLNRFEATAFDPNLAGANVTWDYADADFPEDAYSGEGSIAEAVNLDATFCDLSEILT
                     GNIGYFYEDIDALSYFVKTYNTDKISEEGFKSCMTEYGRLNIYTNPKDYLLFPMEYGD
                     SFSDSFSALTEGYSPFDTSYGSFTGDITVEYDGYGTLILPFITIPNVMRIKTHQVIDY
                     LPSGTYTITDGWYWYNTATKGEIFGYVELFNSDGPDSETFILNQNINQLTSIEMPISI
                     PEIEVFPNPNTGSFNIHFSDNTDRLVQINVFDLTGRLIYNSSVYASNYSSQVSLPDNF
                     SGLAVLTVNDGVSVKSVEISVVK"
     gene            complement(4942..6456)
                     /locus_tag="PLB46_07385"
     CDS             complement(4942..6456)
                     /locus_tag="PLB46_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76378.1"
                     /translation="MKRSLIALITFCNFSFNNAYSQITGALDNSFSDDGIAVIPYDDD
                     NNGLASTLIQPDGKILIIGNEILSFGSINITRLNNDGSIDESFGDGGFVKTSIGEPDT
                     YLHCYAATLLPDGKILVVGQYDDGLSGYPVVLKYNADGTLDLSFGVDGFSVNMLIPNP
                     ASFFDVCLLPDGKFVCAGSSYFGLSNDLLLARFNTDGSIDNTFDDDGFLTSSDFGANT
                     VDLTSEGDIIVGGGSSFGKFKMMKFHSNGSLDLTFGIDGITNFDAHETGFTNAISKIE
                     VLDDNAIIAAGNSDNWGNYVMILSKFKSDGTIDLTFATDGIKIIDFGGSGSPFTDFEL
                     TSDGKITMLGYKILAGGDNEVTLSRYKPDGTIDFEFGDGGSIITLINGQDLNAIAIAI
                     QADDKIVVAGNYSSYEVFVLRYLSGAPSLISLFEETEIKIFPNPSTTSFQFANSSNSN
                     TTIQVNIYDDTNHFICSFNTAYNKSNDVSKLLPGIYLITLLEGSNSSSYSLIIQ"
BASE COUNT         2263 a         1144 c         1198 g         2198 t
ORIGIN      
        1 actgctacat ttacctatcc ggtcggcgaa tggtttgagg cagaacatat tattgacctc
       61 gatgccaata ctatggaaat ttattatgat ggcgtgctgg ttttatccta cgattatccc
      121 ggcagtatcg gtgctatcga tttttattcg gcagctgcaa ataataaatg ttatatcgat
      181 gatgttgtgt taatggaaga attacctgcg gaagcttgtg ctattcctgg tgccattttc
      241 tgtgataata ttgatacata cactgcaggt gatgcggttg gtccttatgc cgattggtgg
      301 agtacctgga gtggtgttga aggtggtgcc gaagatggta ctgtttctga tgcttatgct
      361 tactcaggag aaaattctgt tttaattccg ggaaccggta ctactgatgc tttattatta
      421 ttagataata tggcatctgg tattaaacgt ttggaatggc aaatgtttat tccttccggt
      481 aaaactgcgt attataatat tcaggaatct gaagttgcag gtattgcatg gaatttagaa
      541 ttgtatttcg gagatattac tgaaggtcag ggtgaattta ctgttcctgc tgctggacca
      601 acatttactt atcctgtaga tgaatggttt ttagtagaac atattgttga cctcgatgcc
      661 gataatatca aagtatatat tgatggtgtg atggttttag atgatgtata tacaggtagt
      721 ttgggaagta tcgatttctt ctcatggtct gcatctaact cttattattt agatgatatt
      781 ttatatatcg aagaagatgt tgttgtagca gaaccttgtg atgttccggg agctgtattc
      841 tgcgataata tggatactta tgaagaaggt gctttaggcc caaatgctga ctggtggagt
      901 acctggagcg gtgttgaagg tggtgctgaa gatggtttag tgagctcaga ttttgcagca
      961 tctgcaccaa attctgcatt aattcctgaa ggtggtgtta ctgatgttat tttaaaatta
     1021 ggaaatttat catcaggaaa atataattta tattggaata tgtatgtgcc atcaggcagt
     1081 catgcttatt acaatattca ggaaactgaa gttccgggtg ttgcttggaa tgcagaatta
     1141 tattttggat tagctacacc tggtgaaggt gaaattactg ttccggcaga attaacattt
     1201 acttatcctc ctgatgcatg gttccttatt gaacatatta tcgacatgga tgccaatact
     1261 attgagatta aacttaatgg tactgtaata ctaagtgaag tatatgaagg aaatatgggc
     1321 ggtgttgatt tttattctat tgatgcactc aacagatatt atatcgatga tgttgcatta
     1381 atgccaatgg atcctacaga tttaaatact tattatcagg atgcagatag cgatacttat
     1441 ggtaatgcag atgcatcaat aactgtagca ggtgatgcgc caacaggtta tgtagaaaat
     1501 aatttggatt gtgatgatac taatcctgat atttatcctg gagctacaga aatattgaat
     1561 aataaagacg acgattgtga tgaattaatc gacgaaggat taatcaatat cgagacatta
     1621 gctgcaactg gaatggctat tcaggtttat cctgttccgg caaataataa tgttactatc
     1681 gaattaatga gtttaatgag cggcgaagct gcaactttcg aaatttataa tactacaggt
     1741 caggttgttt ataccgaaca attggtatta aacacatcaa ctttagtaaa acaaataaat
     1801 atcgaatcgt taggttcagg aatttatatg gtaaaattaa cttccggtga acataccgta
     1861 aacggacaaa ttatagttca acactaataa taaaatccat ttcacaaagc cgctgataac
     1921 cctatcagcg gcttttttat taaacctccc cgaaacttcc cgccgtcccg ccttcccggc
     1981 aaaaaaacta cctacctcca aataaccagc ttagcatcct ccaaccctat ataaggcaca
     2041 aacccacatt ccaccccata atgttctctc ataaactctc ggtaattact ataaaacgct
     2101 gcatcattac taataaaaac cgcaggttta cccacaatat ctattgtaag gttttgaata
     2161 ttatttttcg ccaataaact atggtaagta ggcgtattaa taaacgaagc cccatctatt
     2221 aaattattcc ctattatcct cggagcagga gcaaacaacg aaggccgatg cagctggtca
     2281 gaataatcga tatcaataaa taatgtttca ggatgtaatc ccaccaattt aatagcattt
     2341 tcatttttgg cagtcagctc cttgtgataa ttactaatat tgcgattgat accggtttgc
     2401 ataattgcaa ttacaagtaa aaacaaacct atcactttac tttttaacga taaattagtt
     2461 gtttgtatac gattacttac ctcgtataaa atcatcatca ccacggtaaa acacaatggt
     2521 aaatacaaac gcaaaggaag tttgaggtaa cttgccagca ataataaaag cagatgaata
     2581 aaaacggtat aaataataat tttttttctt tgtcccgcat tagcgaataa aaaacaaaac
     2641 ccaataatta ccaataaagc cccataaaat aaaaaagatg tagcataatt aactaagaca
     2701 tcagcaccct taggtaaatc cagcaaattg gattttaaac cggcaagttg tacaattttt
     2761 tcctgcgtaa atactttatc atctgcccaa aagtaggtgt ttaataactt gtaatcggta
     2821 gtggttaagt ccacctgttg caaagtgttt gctgacattt cactatcata atccagaatt
     2881 ttatgcgtag caagttgata ttggatataa ttatgcgtag atgagataga tgcataacac
     2941 tgcagattta catatattag aagcaacggg aataataatg ttacaccata aataaaatgt
     3001 ttttttgaaa acacaggctt gcgaaaatga taaattaaaa ctggtgctgt tataactaat
     3061 aatcccacat aactttccca acgaacgagg acacataaca ataaaaagcc cgtcattttt
     3121 aaaatggata aaggtttggt agtattattt ttaatgagat gcaagagggt taataacccg
     3181 gcaatacccg ctaaaaaggc tgtggaagta aattgaatat taataattac atacactaca
     3241 aatatggcat atatcagtat atacaagccg aaaaacaatc ttgaactatt tgctttattc
     3301 agcacatagg taatttgcac aaaagccaac agttgaatac tatatagaaa cagtggatac
     3361 caattaattc ccggaaaccg cacaaacaac cattttaaaa agaccccttc caaataattc
     3421 gaaaacacca gatattgcga aggctctcca ttcaatgtgc ctgcagcaat ataattcatc
     3481 atcgtatcgt cactggtttc ataacttgga tgaaatacaa ccaataccac agcaaatagc
     3541 gctataacaa tggcgatagc gtattgggtg gggatggagg tttggagggg cttcagattc
     3601 acaatttcag tttaaaatta ttaaaaattg ctgataaggt atctgaatat ggttggcctt
     3661 tttgataata ttaagtttat atcccattta tgattataaa caatattttt ttatgtttgt
     3721 agacagaaaa caattaacta cacatcctac ttttaattta ttactatgaa aacttcaaac
     3781 aaacgcacct tttttattca gttggctatt ttttttataa ttgggattag tggcatttca
     3841 tattcctata cacagcctgt tatcaactac ggtatctacc ctttagtggg tgaatcttat
     3901 aatctcaata ggtttgaagc cactgctttc gatccgaatt tggctggggc aaatgttact
     3961 tgggattacg ctgatgctga ttttccggaa gatgcttatt ccggcgaggg aagtattgca
     4021 gaggctgtta atttggatgc gacattttgc gatttatcgg aaattctaac tggaaatata
     4081 ggttattttt acgaggacat tgatgctttg agctactttg tcaaaacgta taacacggat
     4141 aaaatttcag aagaaggttt taaatcctgc atgacagagt atggtaggtt gaacatttat
     4201 accaatccta aagattacct tttgtttcca atggaatatg gcgattcttt ttcagattca
     4261 tttagtgcat tgacggaggg atattcgcca tttgatacct cctatggatc atttaccggc
     4321 gatattacgg ttgaatatga tgggtatgga acacttattt tgccatttat aaccatacct
     4381 aatgttatgc ggattaaaac ccatcaggta atagattatt taccttccgg aacttataca
     4441 ataactgatg gttggtattg gtataatact gctacaaagg gagaaatatt tggttatgtg
     4501 gaacttttta atagcgatgg gccagactca gaaacattta ttttaaacca aaatattaat
     4561 caattaacaa gtattgaaat gccaatttca attccggaaa ttgaagtttt tcctaatcca
     4621 aatacaggta gctttaatat acatttttct gataacactg accggttagt ccaaatcaat
     4681 gtttttgact taaccggcag attaatttat aattcatctg tttatgctag taactattcc
     4741 tcacaggtca gtttgcctga taacttttca ggtttagcag ttttaactgt caatgatgga
     4801 gtctcagtaa aatctgtgga aatatctgtt gtaaaataac ttttcgcagg ttattgaata
     4861 taacttattg acaataaaaa ataactcata tattgaaaat tgttataggt attgtggttt
     4921 tctatattat gaattaacat tttattgaat gattaacgaa taactggacg aatttgagcc
     4981 ttccaataat gttattaaat aaataccagg caatagtttt gatacatcat tacttttatt
     5041 gtaagcagta ttgaaagagc atataaaatg atttgtatcg tcatagatat taacctggat
     5101 tgttgtattt gaattagagc tattagcaaa ttgaaaacta gttgtacttg gattaggaaa
     5161 aattttgatt tctgtttcct caaataaact aattagtgat ggagcgcctg ataaataccg
     5221 taacacgaat acttcataac tagaataatt accagccaca acaattttat catcagcttg
     5281 aattgcgata gcaattgcgt ttaaatcttg gccatttatt aaagtaataa tggagccgcc
     5341 atctccgaat tcaaaatcaa tagtgccatc tggtttatat cttgaaagtg ttacttcatt
     5401 gtctccaccc gctaaaattt tatagcccag catagtaatt ttaccatcag aagttaattc
     5461 aaaatcagta aatggactcc cactgcctcc aaaatctatg atttttattc catcggttgc
     5521 aaaggtaagg tcaattgttc catctgattt aaatttagat aatatcatta catagtttcc
     5581 ccaattgtcg gaatttccag cggcaataat agcgttatca tctaaaacct caattttgct
     5641 tatagcattt gtgaagccag tttcgtgagc atcaaaattt gtaataccat caataccaaa
     5701 agttaaatct aaacttccat ttgaatggaa tttcatcatt ttaaacttgc caaaagaaga
     5761 accacctcca acaataatat cgccttcaga agtaaggtca actgtatttg caccaaaatc
     5821 tgaactggta agaaaaccat catcatcaaa ggtattatct atactgccat cagtattaaa
     5881 acgagccagc aataaatcat tagataatcc gaaataggaa gaaccggcgc aaacaaattt
     5941 accatcgggt aggagacata catcaaaaaa tgaggcgggg tttggaataa gcatgttaac
     6001 ggaaaatcca tccacaccaa aactcaaatc caaggtaccg tcagcattgt attttaacac
     6061 cacaggatat cctgataatc catcatcata ttgtccaact acaagtattt ttccatcagg
     6121 gagtaaggta gccgcataac aatgtaaata agtatctggt tcgccaattg atgttttaac
     6181 aaaaccgcca tcgccaaagg attcgtcaat actcccatca ttatttaaac gcgttatatt
     6241 aattgaaccg aatgataaaa tctcatttcc gataattaat attttcccat ctggctgtat
     6301 taaggtggag gcaaggccat tattatcatc atcatatggg attacagcta ttccatcgtc
     6361 actgaacgaa ttgtccaggg cacctgtaat ttgggaataa gcattattga atgaaaaatt
     6421 acaaaaagta ataagggcaa tcaagctgcg tttcataggt ggattttaat ttgtatgata
     6481 aagttaatta ttttataatt atgtgcaaag tttcaatggg ggtttttacc agatgattag
     6541 ttatctcaaa tacgtaaaaa gaacctaaaa attgcatgga ttttaaaaaa aacggaattc
     6601 aaattctctt gctatcggta agtatttaaa taatttcgta ttaccagcaa gcctattcaa
     6661 tttcatttaa aacgtgggct atgcaactct ttagttgcaa tcatagaata ctaattaacc
     6721 aaaatataat ttaggaaata atattattgg ctcagtaagg aaacttatct ttctatcccc
     6781 aaaatttttt atctttccat taa
//
LOCUS       DAORKW010000150         6795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_375_length_6795_cov_4.952671, whole genome shotgun sequence.
ACCESSION   DAORKW010000150 DAORKW010000000
VERSION     DAORKW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_375_length_6795_cov_4.952671"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLB46_07390"
     CDS             <1..400
                     /locus_tag="PLB46_07390"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76379.1"
                     /translation="SILASEADLETKIRSLVHAEIDMLLKNQDLPLFIISEASRNPEM
                     MIQQMTNVPVRQFMLQFVQLINTEAAKGNIKAINPIHLLMHIMSLCIFPFVGRPVFMH
                     VTGTDMNGFENLMEERKNIIADSVIQLIKK"
     gene            406..1674
                     /locus_tag="PLB46_07395"
     CDS             406..1674
                     /locus_tag="PLB46_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRF76380.1"
                     /translation="MKKFGLVVYVLFVLTSSNYAQSTVTLNTCYSLAETHYPFVAQKN
                     ILAQITDEAIQKINAVWQPQVYINAQATYQSDVTSIGITLPGIEIQELSKDQYKATLD
                     ISQTLYDGGISKQQRAIQTINTDVETQKVNVDMYKIRERINQIYLVILQAEKQLALVD
                     NLKIELNNQAEKIKAGQQFGTATQFQADILAAEIIKADQKRIEIISAREAAINMLNQI
                     TGSEFNYQTQFETPVIGLAENDTIIQRPELALYDAQSKLLLSQMSFAEAATLPKLSLF
                     VQGGYGRPGLNFLDDTFQFYYLGGIKLNYPLWTGNIKSHDAAIYRLQADNIENYKSSF
                     TLTISVQSEQQLAEIKKVNQLIEKDQELITLKTKIKTGAQAQLDNGTMAADDYVKYVT
                     DENEAKINLAIHQMQLVAAQINYLTIIGKL"
     gene            1679..2575
                     /locus_tag="PLB46_07400"
     CDS             1679..2575
                     /locus_tag="PLB46_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRF76381.1"
                     /translation="MKKTLIILSATILLASCAGEENAFDATGTFEAKEIIVSTGVGGK
                     IIQLKAEEGDSLSANAIVGTIDTTQLYLKKLQLQAQIKAVLSKQPDINSQIAGLQEQI
                     KTANTEKIRIDNLVAAGVGTQKQADDIRSQITVLQKQLDAQQTALQITSQSIYQEVAP
                     LTLQIAQVDDQLVQSAILNPVKGTVLTLYAEQGEFTTPGKAIYKIADLSTMTLRAYIT
                     GAQLAQIKLGQQVTVKIDNGEKNYSELPGTIYWISEKAEFTPKTIQTKEERANLVYAI
                     KVKVPNNGQIKIGMYGELILNK"
     gene            2577..3491
                     /locus_tag="PLB46_07405"
     CDS             2577..3491
                     /locus_tag="PLB46_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF76382.1"
                     /translation="MSGIQIQHINKSFNKGKVQALTDISFTVRPGELFGLIGPDGAGK
                     TTLFRILTTLWLADSGTANVNGLDVIKDYAAIRKIIGYMPGKFSLYQDLTVEENLQFF
                     ATLFHTSIEKNYDLIKDIYIQLEPFKTRKAGKLSGGMKQKLALCCALIHKPQVLFLDE
                     PTTGVDPVSRKEFWEMLKRLQKELTIVVSTPYMDEANLCDRIALIQKGHILQIDKPAA
                     IIQAFDKKLFAVKSSNMHRLISDVRNLPEVNSCFGFGDYLHVTTNTDSTDENHLIAAI
                     TAQQHTDIEVKIIQPEIEDCFMALMEKE"
     gene            3496..4239
                     /locus_tag="PLB46_07410"
     CDS             3496..4239
                     /locus_tag="PLB46_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF76383.1"
                     /translation="MTDKVIITENLTKKFGDFTAVDGISFHVNKGEIFGFLGANGAGK
                     TTAMKMLIGIWEPTSGKAEVAGYDIYRDMENIKKSIGYMSQRFSLYEDIPVIENIRFF
                     GGIYGLSRAEIKEKSAALLHKLNLTAESKTLVSALPLGWKQKLAFSLAIIHQPKIVFL
                     DEPTGGVDPITRRKFWELIYDAANEGVTVFVTTHYMDEAEYCDRISIMVDGRIAALDT
                     PAALKKQFNAENIDQIFTHLARGAKRTAD"
     gene            4241..5347
                     /locus_tag="PLB46_07415"
     CDS             4241..5347
                     /locus_tag="PLB46_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF76384.1"
                     /translation="MQQFWVFVRKEFYHIFRDKKSLLIIFALPIAQMLIFGFALTNEI
                     KNTQIGVLDNANDEASLILIAQLEASKYFEISNTFTNPDEIDAAFKADKIKLAIVIPP
                     NFKSELAHNNSARIQLLADATDPNTATTLEVYASAVIRDYQNTINNNFKLPYSISTEI
                     RMLYNPQLKGAYLFVPGVMGMILLLISAMMTSISIVREKELNTMEIILASPLKPMVLI
                     LSKAVPYMVISLINVSTILLISVTLLDLPINGSIWLVFGVCFLFITTALSLGLMISNI
                     TDSQQTAMFISLMGLMLPTLILSGFMFPIENMPVPMQILSNIVPTKWFFIIIKDVMIK
                     GLGFSSVWREILILAGMTAFFLIISWKKFKMRLA"
     gene            5344..6462
                     /locus_tag="PLB46_07420"
     CDS             5344..6462
                     /locus_tag="PLB46_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF76385.1"
                     /translation="MRILLVILRKEFQQIMRNPSILRIIFMMPAVQLLILPLAADYEV
                     KNVNLTVVDQDHSTYSQLLLNKLSASDYFIIQDVADNYDAAMVDIEKDVSDIILTIPP
                     HFERDLIREDEAKLSMAVNAVNGAKGNIGASYAANIINEYNQEIRKTWFTMPEFNPVP
                     IIDITYSNWYNDGMSYTLFMVPGILVILLTLVGSFLSALNIVHEKEVGTIEQINVSPI
                     KKYQFILGKLIPFWLIGFVVLTIGLLIARFVYGIIPAGNMGLIYLFAAVFLFALLGIG
                     LLVSTIAESQQQAMFISFFFMMIFILMGGLYTSIDSMPEWAQVITWFNPVKYFIEVMR
                     MIVMKDSSFADISHNLLVIGIMAVVFNTWAVLLYRKRV"
     gene            complement(6627..>6795)
                     /locus_tag="PLB46_07425"
     CDS             complement(6627..>6795)
                     /locus_tag="PLB46_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRF76386.1"
                     /translation="GNIIIRQRGTEFHPGKNVGMGKDHTIFALVEGVVTFTKGRKGRS
                     FVNVIPAEAEA"
BASE COUNT         2314 a         1161 c         1276 g         2044 t
ORIGIN      
        1 gtcaatttta gcgtccgagg ctgacctgga gaccaaaatc cggagtttgg tgcatgctga
       61 aatcgatatg ttacttaaaa atcaggatct ccccttattt atcatcagtg aagcaagccg
      121 caatcctgaa atgatgatac aacaaatgac aaatgttccg gtaaggcaat ttatgctgca
      181 atttgtccaa ctcattaaca ctgaggcggc aaaaggtaat ataaaagcca tcaaccctat
      241 ccatttgctc atgcacatta tgtcgttatg tattttccct tttgtcggaa ggccggtatt
      301 tatgcatgtt acgggaaccg atatgaacgg ttttgaaaac ctgatggaag aaagaaaaaa
      361 tattatcgcg gattctgtaa tacaattgat aaaaaaataa tggttatgaa aaaatttggt
      421 ttagttgtgt atgtgctttt tgtgctaaca tcctccaact atgcacaatc cactgttaca
      481 cttaatactt gttatagttt agctgaaacg cattatccgt ttgttgctca aaaaaatatt
      541 ctggcacaaa taacagatga agccatacaa aaaataaatg ccgtatggca accacaggtg
      601 tatattaatg cgcaagccac ttatcaaagt gatgttactt caataggtat tacgcttccc
      661 ggaattgaaa tacaagagtt gagtaaagac cagtataaag caacactgga tatttcacaa
      721 actttatatg atggtggtat cagtaaacaa caacgcgcca tacaaacaat aaataccgat
      781 gtggaaacac agaaagtaaa tgtagacatg tataaaattc gcgaacgtat caatcaaatt
      841 tatttggtaa tccttcaggc agaaaaacaa ctagcattag tagacaatct gaaaattgaa
      901 ttaaataatc aggcagaaaa aataaaagcc ggacaacaat ttggaaccgc aacgcagttt
      961 caggcagata tacttgcagc agaaattatt aaagcggacc aaaaacgtat cgaaataatt
     1021 tccgcacgtg aagcagccat taacatgttg aatcaaataa caggtagcga atttaactat
     1081 caaacccaat ttgaaacacc ggttattggt ttagcagaaa acgataccat tatccaaaga
     1141 cctgagttag cgttatacga tgcacaaagc aaattattac tatcacaaat gtcatttgca
     1201 gaggctgcta ccttacctaa actgagtttg tttgttcaag gcggatacgg acgccccggg
     1261 ttaaattttt tggatgatac atttcagttt tattatttgg gaggaataaa attaaattat
     1321 ccattgtgga caggcaatat caaatcacat gatgccgcaa tttaccgttt acaggccgat
     1381 aatattgaaa actacaagtc ctcctttaca ctaactatat cagtgcaatc tgaacagcaa
     1441 ctagcagaaa taaaaaaagt taatcaactg attgaaaaag accaggaact aattaccctt
     1501 aaaactaaaa taaaaacagg cgcacaagca caacttgata acggcacaat ggcagctgat
     1561 gattatgtga aatatgtaac tgatgaaaat gaagccaaaa taaatcttgc cattcatcaa
     1621 atgcaattgg ttgcggctca aattaattat ctgacaatta ttggtaaatt ataagtatat
     1681 gaaaaaaacc ttaatcatac tgagtgcaac aattttgtta gcaagttgcg ccggtgaaga
     1741 aaatgctttc gatgctaccg gcacattcga agcaaaagaa ataattgttt caacaggcgt
     1801 aggcggaaaa attattcaat taaaagcaga agaaggcgac agtttatcag ctaatgcaat
     1861 agttggaaca atagatacca cacagctcta tttaaaaaaa ttgcagctac aggcacaaat
     1921 taaagcggta ttaagcaaac aaccggatat taattctcaa attgccggat tgcaggaaca
     1981 aattaaaact gccaatactg aaaaaatccg tatcgacaat ttggttgcag caggtgttgg
     2041 cacacaaaaa caagcagatg atattcgcag tcagattact gtgttacaaa aacaattgga
     2101 tgctcaacaa actgcgttgc aaattacctc tcaatcaatt tatcaggaag ttgctccgct
     2161 tacattgcaa attgcacaag tggatgacca attagtgcaa tcagcaatac tcaatccggt
     2221 aaaaggaaca gtgcttacat tgtatgcaga acaaggcgaa tttactactc cgggaaaagc
     2281 aatttataaa attgcagatt taagtaccat gaccttacgt gcctatatta ccggagctca
     2341 gctagcacaa attaaacttg gtcaacaggt taccgttaaa attgataacg gcgaaaaaaa
     2401 ttattcggaa ttaccgggca ctatttattg gatttcagaa aaagcggaat tcactcccaa
     2461 aaccattcaa actaaagagg aacgtgccaa tttagtgtat gccattaaag taaaagtacc
     2521 aaataacggc caaataaaaa ttggcatgta tggcgaattg atattgaata aataatatga
     2581 gcggtattca aatccaacac attaataagt cctttaacaa aggaaaagtg caggctttaa
     2641 ccgatatttc gtttactgtc cggcccggtg aattatttgg attaatagga ccggacggtg
     2701 caggaaaaac aactttgttt cgtatactca cgacgctttg gttggcagac agtggaacag
     2761 cgaatgtgaa tggccttgat gtgataaaag attacgctgc gatacgcaaa attattggct
     2821 atatgccagg taaattttct ttgtatcagg atttaactgt tgaagaaaat ttacagtttt
     2881 tcgctacatt atttcatact tccattgaaa aaaattatga cctgataaag gatatttata
     2941 ttcagctgga accttttaaa acacggaaag caggtaaatt atcaggtggc atgaaacaaa
     3001 aactggcatt gtgttgcgca ctcattcaca agcctcaagt tttattttta gatgaaccaa
     3061 caaccggcgt tgaccctgtt tcgagaaaag aattttggga gatgttaaaa cgattgcaaa
     3121 aagaactgac tatcgttgta tctacacctt atatggatga agcaaattta tgtgacagga
     3181 ttgcattaat tcaaaaagga catattttac aaattgacaa accggcagcc attatccagg
     3241 cattcgacaa aaagttattt gcggttaaat cgtcaaatat gcaccgattg attagtgatg
     3301 ttcgcaattt accggaagta aacagttgtt tcggatttgg cgattatttg cacgttacca
     3361 caaacactga cagcaccgac gaaaatcatt taattgcagc tataactgct caacaacata
     3421 ccgatatcga agtaaaaata attcaacccg aaattgaaga ttgttttatg gcattaatgg
     3481 aaaaggaata acaacatgac agataaagta atcataacag aaaacttgac caaaaaattc
     3541 ggcgacttta cagccgttga tggtatttca tttcatgtaa ataagggtga aatatttgga
     3601 tttttaggtg ccaatggtgc gggaaaaact actgccatga aaatgctgat tggcatttgg
     3661 gagcctacat ccggtaaagc cgaggttgca ggttatgata tttatcgcga tatggaaaac
     3721 attaaaaaaa gtattggtta tatgagccag cgcttttctt tatatgagga tataccggtt
     3781 attgaaaaca ttcgtttttt tggcgggata tatggattaa gtcgcgcaga aattaaagaa
     3841 aagtccgccg ccttattaca taaattaaat ttaacagcag aatccaaaac attagtaagt
     3901 gcacttcctt tagggtggaa acaaaagctg gctttttcat tggccattat ccatcagcca
     3961 aaaattgttt ttttggatga accaacaggt ggtgtagacc cgattaccag aaggaaattt
     4021 tgggaattaa tttacgatgc agctaacgaa ggtgttactg tgtttgtcac cacacattat
     4081 atggacgaag ctgaatattg cgaccgcata agtattatgg tggatggccg catcgctgca
     4141 ttggatacgc ctgctgcatt aaaaaaacaa tttaatgccg aaaatataga tcaaatattt
     4201 acacatcttg caagaggtgc aaaaagaaca gccgattaat atgcaacaat tttgggtatt
     4261 tgttagaaag gagttttatc atattttccg cgacaaaaaa tcgctgctta tcatttttgc
     4321 attaccgatt gcacaaatgc tgatttttgg ttttgcatta actaatgaaa taaaaaatac
     4381 gcaaatcggt gttttagata atgccaatga cgaagcttcg cttattctca tcgcccaact
     4441 ggaagccagt aaatattttg aaataagcaa cacatttacc aatcccgacg aaattgatgc
     4501 agcatttaaa gctgataaaa ttaaacttgc tattgtaatc ccacctaatt ttaaatcaga
     4561 actggcacat aataattcag ctcgtataca attgttggct gatgccactg acccaaatac
     4621 agcaactacc ttagaagtat acgccagtgc agttatccgc gattatcaaa atacgatcaa
     4681 taataatttc aaactcccct attccatttc gacagaaatt aggatgctgt ataatccgca
     4741 attaaagggt gcctatttat ttgtaccggg cgttatgggt atgattttat tattgatttc
     4801 cgcaatgatg acatctattt caattgtgcg agaaaaggaa ttaaatacca tggaaattat
     4861 tttagcatct ccattaaaac ctatggtact cattcttagt aaagccgtgc cgtatatggt
     4921 aatttctcta atcaacgttt ctactatttt attaatcagt gtaacacttt tggacctgcc
     4981 tataaacggt agtatatggt tagtatttgg tgtatgtttt ttgtttatta caactgcatt
     5041 gtcattggga cttatgatta gtaatataac ggattcacaa caaacggcaa tgtttatttc
     5101 gctgatgggt ttgatgttac ctacattaat attgagcggg tttatgtttc cgattgaaaa
     5161 tatgccggta ccaatgcaga tattgtccaa cattgtacct actaagtggt tttttattat
     5221 tataaaagat gtgatgatta agggattagg atttagtagt gtttggcgcg aaatattaat
     5281 attagcagga atgacggcat ttttcctgat aatcagttgg aaaaaattta aaatgcgatt
     5341 ggcatgagga ttttattagt gatattacga aaagagtttc agcagataat gcggaaccca
     5401 tctatattgc gtatcatatt tatgatgccg gctgtgcagt tattgattct gccacttgct
     5461 gcagattatg aagtaaaaaa tgtaaacctc accgttgtag accaggacca ttctacctac
     5521 tcacaattat tattaaacaa attaagtgct tccgactatt ttatcatcca agatgtagct
     5581 gataattatg acgcagctat ggttgatata gaaaaggatg tttcggatat tattttaacc
     5641 ataccgccgc attttgagcg ggatttaatc cgggaagatg aagccaaact atcaatggca
     5701 gttaatgcgg taaacggagc aaagggaaat atcggtgcat cttatgcggc aaatattatt
     5761 aatgaataca atcaggaaat tcgcaaaaca tggtttacca tgcctgaatt taatcctgtc
     5821 ccgataatcg atatcactta tagtaattgg tataacgatg gcatgagtta taccttgttt
     5881 atggttccgg gtattttagt aattttatta accctggttg gcagtttttt atcagcatta
     5941 aatattgtac atgaaaaaga agtcggtacg attgaacaaa taaatgtctc acccattaaa
     6001 aaataccagt ttattttagg caaactgatt ccattttggt taataggttt tgttgtttta
     6061 accattggat tattaattgc ccgttttgta tacggtatta ttcctgccgg caatatggga
     6121 ttgatttatt tatttgctgc agtattttta tttgcgttat taggaattgg attattagtc
     6181 tccactattg ccgaatcaca acaacaggcc atgtttatct ctttcttttt tatgatgata
     6241 tttattctga tgggtggcct ctatacatca attgatagca tgccggaatg ggctcaggta
     6301 attacatggt ttaatccggt aaaatatttt attgaagtaa tgcgtatgat tgtgatgaag
     6361 gatagcagtt tcgccgatat tagtcacaac ttgctcgtaa ttggaattat ggctgtggta
     6421 tttaatactt gggcggtgtt gttgtatcgg aaaagagtgt agcgcaacac aaactatcgt
     6481 attcaaagta attaattccc attattgttg aatactcagt tgcaggtata cctattaatt
     6541 gattaattat aatacatgta attataaggc acaaaaaaag aggctatctt ttggagacag
     6601 cctcttgaaa tatgttgtat tcggaattaa gcttcagctt ctgccggaat tacatttacg
     6661 aatgaacgac ctttgcgacc ttttgtgaaa gttactacac cttcaactaa tgcaaaaatg
     6721 gtatgatctt tacccattcc tacgttttta ccgggatgga attcagtgcc tctttgacgg
     6781 atgataatat tacct
//
LOCUS       DAORKW010000151         6789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_376_length_6789_cov_6.156965, whole genome shotgun sequence.
ACCESSION   DAORKW010000151 DAORKW010000000
VERSION     DAORKW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_376_length_6789_cov_6.156965"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLB46_07430"
     CDS             <1..620
                     /locus_tag="PLB46_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HRF76387.1"
                     /translation="GSADIEVIWKSYQLDPNQEDVPGKSINQYLAERKGWTVDYAREM
                     NKYVTDMAAGEGLVYDMDRAVVANSFNAHRIIQMAKAIGKGDLAEETFFKAYFTEGKN
                     INDTAVLIEIGLSLGLTQSDIESMLDSGLYVNEVQADAHEAAALGARGVPFFVFNRKY
                     AVSGAQHVEAFLQVLNTAFSDWKLTQENSPVVINEGDMCDTDGNC"
     gene            670..2187
                     /locus_tag="PLB46_07435"
     CDS             670..2187
                     /locus_tag="PLB46_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76388.1"
                     /translation="MKPSDNLFKLIKSLDKSEKGYFKKFALGHGKNSNYILLFNAIDA
                     QDVYDEEALLKKFKTQAFAKQIAVTKNYLWELILRSQRQYRRDVSKFMQLNGLLENGE
                     ILFEKGLYDEALKQWDKAKNLAIAYNEIPFLLDIETSKRRYYIDMTAGNWQTFVEPSY
                     TYSFKLLDEYRNMLHIQQRYVKIINEIKVQPYFRTDEQKQEWDMFMQDPLLAISNEPD
                     FFWGKLYFNYIHNIYHLLCRNKVEALLYIKKVVALWEQNPELKNMEPIKYLSAVNNYL
                     INLMLLGEKETFIHYFESFVPPPLNSISQEAVYFEHLWLMQNTCFTLKKDFVGMLAFI
                     ESSKADLDKYANYINKVRLLIIRFSMAFGKTMNGNYAGSNDLLQLIFDSKEVELRKDI
                     QATARILYTINHYEQDNLLLIKHVINTAKHFLKNNDSYYETEQVFMKYITRLSKEVGK
                     KEKLAVYLKMEEEVTIIFANNEKERTAFEHLHLLEWIHAKINGISFVDALTERLV"
     gene            complement(2192..2548)
                     /locus_tag="PLB46_07440"
     CDS             complement(2192..2548)
                     /locus_tag="PLB46_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF76389.1"
                     /translation="MSSPLTIAKLWLHAFNEHNLEALLDLYADDAQHFSPKLKIRQPE
                     TNGLIIGKDAMRNWWQDAFERLPDLVYREEQLTANNNRVFMEYTRIVGGEQNISVAEL
                     LEIKDGKIIFSRVYHG"
     gene            complement(2548..3996)
                     /locus_tag="PLB46_07445"
     CDS             complement(2548..3996)
                     /locus_tag="PLB46_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HRF76390.1"
                     /translation="MTNPVRTLQPTALWNHFEDINAVPRPSKKEERIIAFMKAFGESL
                     GLKTVVDSIGNVIIYKAATTGMEDRKTVILQSHLDMVHQKNADTNFDFSTQGIQSYID
                     GEWVKARGTTLGADNGIGVASIMSLLTSTDIPHPAIEALFTIDEETGMTGAFKLDKGL
                     LQGKILLNLDTEDDDEFSIGCAGGIDTNTSYNYTPEPVPANAIAYQITVNGLKGGHSG
                     VDIHLERGNANKIMNRLLYQCEQFGLQIASIDGGSLRNAIPRESFATVIVTDEKFVAE
                     LESRAAAIKNEFSLQDEGLKITISKTEMPHDVMSKSDTQKVTTAIQAVHNGVFCMSSA
                     IAGLVETSSSFARVQIKDGKFITQSLQRSSIESAKYNVAYTVGAPLKLMGCTVEHTGS
                     YPGWAPNPDSAILRLMENKYTELFGEKPHVMAIHAGLECGILGERYHGLDMISFGPTI
                     KHPHSPDEKVNIASVQKFWNFLLAILKDTPKN"
     gene            4161..4955
                     /locus_tag="PLB46_07450"
     CDS             4161..4955
                     /locus_tag="PLB46_07450"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRF76391.1"
                     /translation="MINVANAQSFTTISLNIRYDNPADGVNNWHQRKVELTDFLIHQQ
                     PLFVGVQEALYHQVQFMDSVMKNYSYLGVGRDDGKLAGEFAPVFYDSTRVKLLSANTI
                     WLSETTDTISIGWDAALPRICSYGLFEIKSTQQKIWVFNTHFDHMGEIARAESAKVLL
                     NQIATENRSQYPVILMGDFNCTEKSLPIQYILTQFEDAKLAATLPFEGITGTFNGFDT
                     LSVPEKCIDFIFVQGIHAKSLEHPVTKTKNGLWLSDHLPVVGVLEL"
     gene            5079..5876
                     /locus_tag="PLB46_07455"
     CDS             5079..5876
                     /locus_tag="PLB46_07455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRF76392.1"
                     /translation="MKKLLVYTALTMSLLFGKRVSAQTEVIFYTTMGVFEVELYDTLQ
                     PITAGNFISLVVEKFYDEIIFHRVVEDFVIQGGDPMGTGFGGPGYTIDDEFDPLASNI
                     QKALGMANAGPNTGGSQFYINLVDNTYLDPNYPVFGIVIDHFDVVEAISHVEVNGLDK
                     PLVPVVMDSVRIKQTWPTGINNNVPVLDIFVSPNPATDIIQINASEVCENCTVSLINN
                     TGQLFWEGSFSSDTQIPAADLPTGLYFIIVSNEHQQYLAVKRVAIEH"
     gene            complement(5888..>6789)
                     /locus_tag="PLB46_07460"
     CDS             complement(5888..>6789)
                     /locus_tag="PLB46_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76393.1"
                     /translation="GLLVYACWGAYLFFANSNTVASKSKQILHALLALGITCLVFLPW
                     QIYIQQQFPEEASYERAFTRNHINESIEGFDGSYTYYLHYFPVQYAYGLILLFVVGLY
                     FFFKSKISAIHKIIIFSATIIPYLFFSLLVTTKVLGYVFIIAPFILLICGFAFQGLYE
                     YIITRKNRKPGIIILVILLAACCFINLKYGHLQKLRFKGKSSYNWENREDKMHNTTIY
                     KSLHSDTYNGYIILNCKAMEDPEAMFYSGRNVYSWYPNEKTIDSLQALGYKFASFENF
                     GKQELPKYITEDTSILIIHQQLK"
BASE COUNT         2207 a         1129 c         1248 g         2205 t
ORIGIN      
        1 aagggagtgc agacattgag gtaatttgga agagttatca attggacccc aatcaggagg
       61 atgtgcccgg taaaagtatc aatcaatatt tggccgaacg caaggggtgg actgttgatt
      121 atgcgagaga gatgaataaa tatgtaaccg atatggctgc cggtgaagga cttgtttatg
      181 atatggacag ggctgttgtt gcgaattcgt ttaatgcgca tagaattatt caaatggcta
      241 aagcaatcgg taaaggcgat ttggcagaag aaactttttt caaggcctac tttactgaag
      301 gaaaaaatat taacgataca gcggttttaa ttgaaattgg cctttcgtta ggattaaccc
      361 aatcggacat tgaatctatg cttgatagcg gtttatatgt taatgaagta caagctgatg
      421 cgcatgaggc agcagcactt ggcgcaagag gtgtaccgtt ttttgttttt aacaggaagt
      481 atgcagtatc cggcgcgcag catgtggagg catttttgca ggtgttgaat acagcgttta
      541 gcgactggaa attgacacag gaaaactcgc ctgtcgttat taatgagggt gatatgtgcg
      601 atacggatgg caactgctaa ataattttta ccacagtttt taaatcctat tggttacatt
      661 tgttatacaa tgaaaccatc agataacctt tttaaattaa ttaagtcgct cgacaaaagt
      721 gaaaaaggtt attttaaaaa atttgcttta gggcacggga aaaacagtaa ttatatttta
      781 ttatttaatg caatagatgc acaggatgtg tatgatgaag aggcattatt aaaaaaattt
      841 aaaacgcaag cttttgccaa acaaatagcg gtaaccaaaa attatttatg ggagttgata
      901 ttgcgctctc aacgtcaata ccgaagagat gtttccaaat ttatgcaatt aaacggcctt
      961 ttggaaaatg gtgagatatt gtttgagaaa ggattatatg acgaagcgtt aaaacaatgg
     1021 gataaggcaa aaaatttagc aatagcctac aatgaaattc catttttact tgatatagaa
     1081 acaagcaaaa gacggtatta tattgatatg accgccggta actggcaaac ttttgtagaa
     1141 cctagttata cctatagttt caaattactg gatgagtacc gcaacatgtt acatatccag
     1201 cagcgatatg taaaaattat taatgaaata aaagttcaac cttatttcag aacagatgaa
     1261 cagaaacagg aatgggatat gtttatgcaa gacccattgc tggcaatttc taatgaaccg
     1321 gatttttttt ggggaaaact gtattttaat tatattcata atatttatca tttattatgc
     1381 cggaataaag ttgaagcttt attatatata aaaaaagttg ttgcgttatg ggaacaaaat
     1441 cctgagttaa aaaacatgga acccataaaa tatttatcgg cagtaaataa ttacctgatt
     1501 aatttgatgt tattaggaga aaaagaaaca tttatccact attttgaaag ttttgttcca
     1561 ccaccattaa attcaatttc gcaggaagcc gtttactttg agcatttatg gcttatgcaa
     1621 aatacttgtt ttaccctaaa aaaagatttt gttggtatgt tggcatttat tgaatcttca
     1681 aaggcggatt tggacaaata tgctaattac ataaataaag ttcgattact cattattcgt
     1741 ttcagcatgg cattcggtaa aaccatgaat ggcaattacg caggctcaaa cgatttgttg
     1801 caacttattt ttgattcaaa agaggtggaa ttgagaaaag atattcaggc aacagcacgt
     1861 attttgtata caataaatca ttacgagcag gataatttat tgctcattaa acatgtaata
     1921 aatacggcaa aacatttttt gaaaaataac gattcttatt acgaaaccga gcaggtgttt
     1981 atgaaatata taacccgatt gtcgaaagaa gttgggaaaa aagaaaaact ggcagtttat
     2041 ttgaaaatgg aggaggaagt aacgatcata tttgctaata atgaaaaaga gcgaacagct
     2101 tttgaacact tgcatttgct cgaatggatt cacgcaaaaa ttaatggaat ttcatttgtt
     2161 gatgcactta ctgaacgttt ggtttaacca attaaccatg ataaacccga gaaaatatta
     2221 ttttgccgtc tttaatttcc aacaattcag caaccgaaat attttgttca ccaccaacaa
     2281 ttcgtgtata ctccataaat acccgattat tatttgcagt taattgctcc tctctgtaaa
     2341 ctaaatccgg caaacgttca aacgcatcct gccaccaatt gcgcatggca tctttcccaa
     2401 taattaatcc atttgtttcg ggttgtcgaa tttttaattt cggactaaaa tgttgtgcat
     2461 cgtctgcata caaatcaagt aatgcttcta aattatgttc attaaaagca tgtagccata
     2521 atttcgcaat agttaatggt gacgacatta atttttaggc gtatctttta aaatggctaa
     2581 taaaaaattc caaaattttt gcactgaagc aatattcact ttttcatcag gtgaatgtgg
     2641 gtgtttaatt gttggtccaa atgaaatcat atctaaacca tggtaacgct cgcccaaaat
     2701 accacattcc aaacccgcat gaatagccat tacatgtggt ttttcaccaa acaattcggt
     2761 atatttattt tccattaagc gcaaaatcgc ggagtcagga tttggtgccc aaccaggata
     2821 tgaacccgta tgttcaactg tgcaacccat taattttaat ggtgccccca cggtataagc
     2881 aacattatat tttgctgact caatagatga gcgttgtaaa ctttgcgtaa taaatttgcc
     2941 atctttaatt tgcacacgcg caaatgatga cgaagtttcc accaatccag caattgcaga
     3001 actcatacaa aacacgccat tatgcactgc ctgaatagct gtagttactt tttgagtatc
     3061 cgatttactc attacatcat gaggcatttc agttttacta attgttattt ttaatccctc
     3121 atcctgtaaa ctaaattcat ttttaattgc cgctgccctg ctttccaatt cggcaacaaa
     3181 tttttcatct gttacaatta ccgtagcaaa cgattcgcgt ggaatggcat tgcgcaaact
     3241 tccaccatca atactggcaa tctgcaaacc aaactgttca cattgataaa gtaaacggtt
     3301 cattatttta tttgcattac cacgttccaa atgaatatca acaccactgt gaccaccttt
     3361 taatccgttt acggtaattt gataagcgat tgcatttgcc ggaaccggtt cgggagtata
     3421 attataactt gtattagtat ctattccacc tgcacaacca attgaaaatt catcatcatc
     3481 ttccgtatcg aggttgagta aaattttacc ttgcaataaa cctttatcca atttaaatgc
     3541 gcctgtcata cccgtttctt catcaatagt aaataatgct tctatagcag gatgcggaat
     3601 atctgttgaa gttaataaac tcattatcga tgcaacacca ataccattgt cggcacctaa
     3661 agtagttcct ctggctttaa cccattcacc atcaatataa gattgaattc cctgtgtgct
     3721 aaaatcaaaa ttggtatcgg catttttttg gtgcaccata tcgaggtggg actgcagaat
     3781 aaccgttttg cggtcttcca tgccggtagt tgctgcttta tatataatta cattgccaat
     3841 gctatctact actgttttca gtccaaggct ttcaccaaat gctttcataa atgcgatgat
     3901 acgttcctct tttttcgaag gtcgtggcac tgcattaata tcttcaaaat ggttccaaag
     3961 ggctgtaggt tggagcgttc ttaccggatt tgtcatagtt gttaaattac ggtacaaagg
     4021 taaggaataa aatggatgcg atgttgtttg atttggcaga agctgaagac ataatttatt
     4081 cctcaaaaca actaatttta ttgaaatttt aacaattgta tcgagcgata tatatcactt
     4141 gttgtttttt atgttgcaca atgattaatg ttgcaaatgc tcaatcattt actaccatat
     4201 cattgaatat tcgttatgat aatccggctg atggcgttaa taactggcat caacggaaag
     4261 tagagctgac cgatttttta attcatcaac aaccactttt tgttggtgtt caggaagcgt
     4321 tgtatcatca ggtgcaattt atggatagcg taatgaaaaa ttattcgtat ttaggtgttg
     4381 gacgtgatga tggcaaattg gccggtgagt ttgctcctgt tttttatgat tcaacaaggg
     4441 tgaaattatt atcagcaaat actatttggt tgtccgaaac aacagatacg atttcaatag
     4501 gctgggatgc cgcattgcca agaatatgtt cttatggttt atttgaaata aaatcaacgc
     4561 aacaaaaaat ttgggtgttt aatacgcatt ttgatcatat gggcgaaatt gccagagctg
     4621 agtcggccaa agttttgctt aatcaaattg cgacggaaaa taggtcgcaa tacccggtaa
     4681 ttttaatggg tgattttaat tgcaccgaaa aatctttacc tatccaatat attttaaccc
     4741 aatttgaaga tgcaaaactt gctgcaactt taccatttga aggtataaca ggtacattta
     4801 atggttttga tactttatca gtgcctgaaa aatgtataga ttttattttc gttcaaggta
     4861 ttcatgctaa atcgcttgaa catcctgtta caaaaacgaa aaatggttta tggctttccg
     4921 atcatttacc cgttgtgggg gttttagaat tgtgacaata agcaatatag tttgatgttt
     4981 attcctgcta attttcaata cttaacaact tttaactacc ttggtaccgg agcgacttta
     5041 agcttcaatt attattgcat taaacaacac caaacaatat gaaaaaatta ctggtttata
     5101 cagctcttac aatgagtttg ttatttggaa agcgagttag cgcccaaact gaagttatat
     5161 tttataccac aatgggtgtt tttgaagttg aattatatga cactttgcaa ccaataacag
     5221 caggaaattt tattagtctg gtagttgaaa aattttacga tgaaattatt ttccatcgtg
     5281 tggtagaaga ttttgtaatt cagggaggtg acccgatggg aaccggtttt ggtggcccgg
     5341 gatacactat tgacgatgag tttgatccgc ttgcaagtaa tattcaaaaa gccttaggaa
     5401 tggccaacgc tggtcccaac acaggtggca gtcagtttta tattaactta gtagacaata
     5461 cctacctcga ccctaattat ccggttttcg gcattgtgat tgaccatttt gatgtggttg
     5521 aagcaattag tcacgttgaa gtaaatggat tggataaacc attggtgccg gtagtaatgg
     5581 atagtgtgcg cataaaacaa acatggccga caggaattaa taataatgtt cctgttttgg
     5641 atatatttgt ttcacctaat cctgcgacag atatcattca aataaatgct tctgaagttt
     5701 gtgaaaattg tactgttagt ttaattaata ataccggtca attattttgg gagggcagtt
     5761 ttagcagcga tactcaaatt cctgctgctg atttacctac cggattgtat tttattattg
     5821 ttagcaatga acatcaacaa tatcttgcag taaaaagagt ggcaattgaa cattaataac
     5881 ttaccaatta tttaagttgt tgatgtatta tcagaatgga agtatcttct gttatatatt
     5941 taggtaattc ttgtttgcca aaattttcga aggaagcaaa tttgtagcct aatgcttgta
     6001 atgaatcaat tgttttttca ttaggatacc aagagtacac atttcttcct gaatagaaca
     6061 tagcttcagg gtcttccatg gctttacaat ttagtatgat ataaccattg taggtatcag
     6121 aatgcagtga tttataaatg gtagtgttgt gcattttatc ctccctgttt tcccaattgt
     6181 aacttgattt tcccttaaat cggagttttt gtaaatgccc gtattttaaa ttaataaaac
     6241 aacaagctgc taatagaata accaaaataa taattcccgg ttttcgattt ttacgtgtaa
     6301 ttatatactc atataaacct tgaaaggcaa aaccacaaat gagcaaaata aatggcgcaa
     6361 taataaatac gtatccaagc acctttgttg taacaagtaa tgaaaaaaac aaatatggaa
     6421 ttattgtagc gctaaaaatt attattttgt gaattgctga tattttactc ttaaagaaaa
     6481 aatataaccc aacaacaaaa agtagaatga gaccatatgc atattgcacc gggaaataat
     6541 gtaagtaata agtataactt ccgtcgaaac cttcaatcga ttcgtttata tgatttctgg
     6601 tgaatgctct ctcataactt gcttcctcag ggaattgttg ttgaatgtat atttgccagg
     6661 gcaaaaaaac aaggcaggtt attcccagcg ctaataaggc atgtaaaatt tgtttcgatt
     6721 ttgatgcgac agtgtttgaa ttggcaaaaa ataaataagc tccccaacag gcatacacta
     6781 aaagtcctg
//
LOCUS       DAORKW010000152         6756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_381_length_6756_cov_5.023728, whole genome shotgun sequence.
ACCESSION   DAORKW010000152 DAORKW010000000
VERSION     DAORKW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_381_length_6756_cov_5.023728"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLB46_07465"
     CDS             complement(<1..303)
                     /locus_tag="PLB46_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76394.1"
                     /translation="MSTVQANTHAKAIYFMITSTFLFGIMNVLVKMLQRIPSYELVLF
                     RSLVMLLVSGFLLYRSRIFPFGRSHRWVMIGRGVSGSIALLIFFFSIQHLPLATAVT"
     gene            687..2303
                     /locus_tag="PLB46_07470"
     CDS             687..2303
                     /locus_tag="PLB46_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF76395.1"
                     /translation="MKFLFFLLTVSGFYAGYSQQTATTTKPEETLTEEEAMLKFERFL
                     SIVDDRYMDDVDAGKLVEDAIIEVLTQLDPHSVYFTEEELQQANEPLEGNFEGVGIQF
                     NLLNDTIIVVGVISGGPSEKVGLMPGDKIVQINEEKVAGIGMTNKGVTDRLRGKKGTD
                     VKVYIERNGAKKNLEFKITRDVIPLYSVDAKYMAEPSTGYIRVSKFAATTTEEFKLAL
                     SELGKQGMKNLILDLRGNGGGYLNAAIDMANEFLPANKLIVYTEGRAYPRQDAMSVGN
                     GTMKEGKVIVLIDEGSASASEIVTGALQDWDRALVIGRRSFGKGLVQRSFNLPDGSAM
                     RLTVSRYYTPSGRSIQKSYEEGLEAYHNELNERYRDGELTADTTFHYPDSLTFYTNAK
                     RIVYGGGGIMPDIYIALDTTWATDYYAEVVGKGIVNQFAITYVNKNRDQLNSKYADAF
                     AFNKNFDVDAKLWNEFFAYAEADSVKKDEAQFALCEQKLGQVIKAVIARDLFDFEAYW
                     IVANSTDEAYLKALESLNDDTFNKMKIATK"
     gene            complement(2372..3514)
                     /locus_tag="PLB46_07475"
     CDS             complement(2372..3514)
                     /locus_tag="PLB46_07475"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HRF76396.1"
                     /translation="MKKMIQNFCTGLLCFSVTAVQAQLTLPPSGDNQVAEVTQFMGPV
                     SITINYSSPNVHGPQGEDRTGHIWGELVPYGLANLQFGWSTDANPSPWRGGANMNTTV
                     ELSHDVLVEGKKLKAGKYGLHFIPGPTEWVVIFSSNADSWGSFFYKAEEDVLRVTVKP
                     AANNFHEWLTYEFTDRQSDAAVVALMWENLQVPIQFKVENITAVYIDQIRKELRSDIG
                     FNYMSWVDGANYCVQQNTNLTEALTWAKYAIEEPFFGRKNFTSLSCKANVLNALGKTS
                     QADSIMQIAINDPSASMMDIHYYARGLQAQKKNKEALEVFLINYKKTPTSPVTNLGLA
                     RGYSANGDYKNALKYAKAALQLNPDPQVKTTLENAVKLLEAGKDFN"
     gene            3604..3984
                     /locus_tag="PLB46_07480"
     CDS             3604..3984
                     /locus_tag="PLB46_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF76397.1"
                     /translation="MTNAAFHRQIPVLPVADLKATIKYYKSKLGFSDEWFWEDTDAGC
                     GRNELFMLFNLNPELLQAIQSAAAPFEIIWIVEQVDVIYNEYAQKELAIISEPENKPW
                     GMREFTIKDINGYTIRIAEPIENE"
     gene            3977..4738
                     /locus_tag="PLB46_07485"
     CDS             3977..4738
                     /locus_tag="PLB46_07485"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRF76398.1"
                     /translation="MNNNILKQYKNEVEQTLSSIKSARKPSPDFDLQKYIGTHFYVTG
                     LSMPQLRKAFQHGYSFSNKPLPEQFEIYKYLFFNSEVFDAMLQSLFFCEKYVKQADPE
                     YIFNELIDWIGRIDNWAHSDMLTQHFAYLHEVRPDLVYPHLLEWNKSENPWMRRQSVL
                     SPLCYARFRKVNPPFNKIIALVKPLLNDPDYYVQKGVGWCLRETGIVYPEKTFVFLTE
                     YHHAITSTAFAAAAEKLPAKQKEQLKLLRKKSRKK"
     gene            4735..5364
                     /locus_tag="PLB46_07490"
     CDS             4735..5364
                     /locus_tag="PLB46_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF76399.1"
                     /translation="MTELFEIIKEKLPYLETALIEKMIENSSDVTLPPNQQILREGQY
                     IKTIPLVINGLLKVITSHGDKEILLYYIQPSESCVMSFAFSMSDEPSNIAAFTETETR
                     VLILNAEDVKSWIMSYPSINLLFLNQYNNRYKDLVETIGDLIYEKLDTRIYNYLKQRV
                     AISDSGYVNIPHRQIAEEIASSREVVTRLLKKLEADKKIVQTRDGIKVL"
     gene            5528..5734
                     /locus_tag="PLB46_07495"
     CDS             5528..5734
                     /locus_tag="PLB46_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRF76400.1"
                     /translation="MKKNMGSTDRILRIIIAAVFAILYFTGTVSGTLGLVLLILGAVF
                     VLTSLISFCPLYSIFGLNTCPAKK"
     gene            5885..>6756
                     /locus_tag="PLB46_07500"
     CDS             5885..>6756
                     /locus_tag="PLB46_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HRF76401.1"
                     /translation="MCRYIFTPLFLVAVLFTTTVSAQFNIDFLGQLEYDDQLSNLTGW
                     SDGAGHEYAIVGTEDGTSIVDITDPTNPVEVQFVDGNNSIWREVRTWSHYAYVVTEAG
                     GGLLCIDLSTLPGPVDFNFTDGGVGLNSGHTVFCDENGYVHVFGSNKGVGGDQIFNAN
                     IDPMDPPFAGEVEEWYIHDGFVRGDTLWAGNIYDGWFSVWDISDKTSPELMAQQSTPD
                     NFTHNVWLTDDNNFLFSTDEVTNAVVASYDVSDLTDIKELDQFRANPGTNSIPHNTFV
                     RGDFLVTAYYRDGVV"
BASE COUNT         2188 a         1215 c         1279 g         2074 t
ORIGIN      
        1 agttaccgca gttgctaaag gcaaatgttg aatactgaaa aagaaaatga gtaaggcaat
       61 tgagccggaa acgccacgtc caatcatcac ccagcggtga ctgcgaccaa aagggaaaat
      121 gcggctgcga tacaataaaa aaccactaac caacaacata accagggagc gaaaaagtac
      181 taattcgtag gaaggaatgc gttgcaacat tttaactaac acattcatga taccaaacaa
      241 aaaagtagag gtaatcatga aatatatcgc ctttgcgtga gtatttgcct gcactgtact
      301 caaactaaca ttgggtttta agatttagtg caaaataatt gcgaaatttt atggaataag
      361 ggttgagtta tcaccatatg aacaaaatac tgatgaaacc caaagaagta acttaatacg
      421 gtatttacaa acaggcaacg atataatgtg ctatttggtg aaatacagta ggtgcaaagg
      481 caattattcc aaaattggca gaattacacc atatttgtca ggtttatacg aattgtccat
      541 ttttgttagt agaagcatta acctgcaaaa gttgattaag cagttaaatc ttgttaacgc
      601 gaacctaaat tggcacgaat ctcgtttatc tttaaaatca atgttattta gctcatatct
      661 aaacgcttac gaagaacatg aataaaatga aatttttatt tttcctgctt accgtttccg
      721 gtttttatgc cggttacagc cagcaaactg cgacaactac caaacctgaa gaaaccctta
      781 ccgaggaaga ggctatgctc aaatttgaaa gatttttgag tattgtcgac gacagatata
      841 tggatgatgt agatgccgga aaactcgtgg aagatgctat tattgaagtt ttaactcaac
      901 tcgacccaca ctcggtatat ttcactgaag aagaattaca acaagccaat gaaccactgg
      961 aaggcaattt cgaaggtgtt ggtattcagt ttaatttatt gaatgatacc atcattgtgg
     1021 ttggtgtaat ttcaggtgga ccaagtgaaa aagtgggttt aatgcccgga gataaaatag
     1081 tgcagattaa tgaagaaaaa gtcgccggaa ttggcatgac caacaaaggt gttacagaca
     1141 gattacgcgg gaaaaaaggc actgatgtaa aagtgtatat cgaaagaaat ggcgcgaaaa
     1201 agaatttgga atttaaaatt acaagagatg taattccact ttatagtgta gatgcaaaat
     1261 atatggcaga accctctacc ggatatattc gcgtgagtaa attcgcagca acaactactg
     1321 aagagtttaa actggcacta tctgaattag gtaaacaggg aatgaaaaat ttaattctcg
     1381 atttacgtgg taatggcggt ggttatttaa atgcggctat tgatatggcc aacgaatttt
     1441 taccggctaa taaattaatt gtatacaccg aaggaagagc atatcctcgt caggatgcta
     1501 tgtcagttgg caacggtacc atgaaagaag gtaaagtgat tgttttaatt gacgaaggtt
     1561 ctgcttctgc aagtgaaatt gttactggtg cattacaaga ttgggatcgt gcattagtaa
     1621 ttggaagaag aagttttggt aaaggtctag tgcaacgttc atttaattta cctgacggtt
     1681 cagcaatgcg tttaacagtt tctcgttatt atacaccttc aggtcgttcc atccaaaaat
     1741 cgtatgaaga aggtttggaa gcttatcata atgaattaaa cgaacgttac cgcgatggcg
     1801 aattaactgc agataccact tttcattatc cggattcact cacgttttat accaatgcta
     1861 aacgtattgt ttacggcggt ggtggtatta tgcctgatat ttatattgca ttagatacaa
     1921 catgggctac agattattac gcagaagtag ttggaaaagg aattgtaaat caatttgcga
     1981 ttacttatgt aaataaaaac cgcgaccaat taaacagtaa gtatgccgat gcctttgctt
     2041 ttaataaaaa ctttgatgtt gacgctaaat tgtggaacga atttttcgct tatgcagaag
     2101 cagatagcgt gaaaaaagat gaagcacaat ttgctttatg cgagcaaaag ttaggtcaag
     2161 taattaaagc tgtaattgct cgcgatttat tcgacttcga agcctattgg attgtagcta
     2221 attcaaccga tgaagcttat cttaaagcat tggaaagttt gaatgatgat acatttaata
     2281 aaatgaaaat tgcaactaaa taattaaatt ttcaagccaa taaaaaagcc cgcaataatt
     2341 ttgcgggctt ttttattgga caaataattc atcaattaaa atctttgccg gcttctaata
     2401 atttaaccgc attttcaagt gtggttttta cctgagggtc aggatttaat tgtaatgctg
     2461 ctttagcata ttttaatgca tttttataat caccgttagc tgaatatcct cttgctaaac
     2521 ctaaattcgt aaccggtgat gttggtgttt ttttgtaatt gattaaaaat acttctaaag
     2581 cttctttatt tttcttttgt gcttgtaacc ctctggcata ataatgtata tccatcatcg
     2641 atgcagaagg gtcgttgatg gcaatttgca taatactgtc tgcttggctt gttttaccta
     2701 aggcatttaa tacatttgct ttgcaactta aactggtgaa attttttctg ccaaaaaacg
     2761 gttcttctat ggcatattta gcccaggtta aagcttcagt taaattcgta ttttgttgca
     2821 cacaataatt ggcgccatct acccacgaca tataattaaa accaatatca cttcttaatt
     2881 cttttcttat ttggtcaata tacacagcag taatattttc caccttaaac tgaattggca
     2941 cttgcaaatt ttcccacatc aaggcaacta ctgcagcatc actctgtctg tcggtaaact
     3001 cataggtaag ccattcgtga aaattattag ctgctggttt aaccgttaca cgtaaaacat
     3061 cttcttcagc tttgtagaaa aaacttcccc aagaatcggc attagatgaa aaaattacta
     3121 cccattctgt aggtccggga ataaaatgta acccatattt tcccgcttta agctttttac
     3181 cttctactaa aacatcatgc gacaactcaa cagtagtatt catattggct cctcctcgcc
     3241 aaggtgacgg gttagcatct gttgaccatc caaactgtaa attggctaaa ccataaggca
     3301 ccaactcacc ccaaatatgt ccagttctgt cctccccttg tggcccatgc acatttggcg
     3361 acgaataatt gatagtaata cttacaggtc ccataaattg agttacctca gcaacttgat
     3421 tatcaccgct aggtggaagg gttaattggg cttgcacagc ggttactgaa aaacataata
     3481 aaccggtgca aaaattttgt atcatttttt tcatgtcatc tattttgtga taaaaatatt
     3541 tctattatta ttgatgagaa attaatatac gttaatactt gttaagcaca ctagcaatta
     3601 attatgacaa atgcagcatt tcaccgccag attccggttt tgcccgttgc cgacttaaag
     3661 gctaccatta agtattataa atcaaaatta ggcttttctg atgagtggtt ttgggaagat
     3721 actgatgccg gttgcggaag aaatgaatta tttatgttat ttaatcttaa tccggaacta
     3781 ttacaagcca tacaatctgc cgccgctcca tttgaaatta tttggattgt tgagcaggta
     3841 gatgtcattt ataatgaata tgctcaaaaa gaattagcga taatcagtga gccggaaaat
     3901 aaaccatggg gaatgcgtga gtttactatc aaagatatta acggttatac cattcgtatt
     3961 gcagaaccaa tagaaaatga ataacaacat tttaaaacaa tataaaaacg aagtggaaca
     4021 aacattaagt tccattaaat ccgcacgtaa acctagtcct gatttcgatt tacaaaaata
     4081 tatcggaaca catttttatg ttaccggatt atcgatgccg caattacgca aagcttttca
     4141 acatggttat tcgtttagta acaaaccgtt accagaacaa tttgaaattt ataaatacct
     4201 gttttttaac agcgaagtgt ttgatgctat gttacaatcg ctgttttttt gcgaaaaata
     4261 tgtaaaacaa gctgaccctg aatatatatt taacgaactg attgattgga taggccgaat
     4321 cgacaactgg gcacatagcg atatgctgac acaacatttt gcttacctgc acgaggttcg
     4381 acccgattta gtttatccgc atttattgga atggaataaa tccgaaaacc cctggatgcg
     4441 ccgacaatcg gtattaagcc cattgtgtta tgcgcgtttc agaaaagtaa atccaccgtt
     4501 caataaaatt atcgcgttgg taaaaccgct gctcaacgac ccggattatt atgtgcaaaa
     4561 aggtgtgggc tggtgtttgc gcgaaacagg aattgtttac ccggaaaaaa cattcgtatt
     4621 tttaaccgag tatcaccatg ctattacatc gactgctttt gcggcggctg cagaaaaatt
     4681 accggcgaaa caaaaggaac aattaaaatt attgcgtaaa aaatcacgaa aaaaatgacc
     4741 gaattattcg aaataataaa agaaaagtta ccttatcttg aaacagcact cattgaaaaa
     4801 atgattgaaa attccagcga tgtaacctta cctcctaatc aacaaatttt gcgcgaagga
     4861 caatatatta aaaccattcc attggtgata aatggtttat tgaaagtaat tacaagtcat
     4921 ggcgacaaag aaatattatt gtattacata caaccttccg aaagttgtgt gatgtcgttt
     4981 gccttcagca tgtccgatga accaagcaac atagctgcat ttacagaaac tgaaacacgt
     5041 gtactcattt taaatgctga agatgtaaaa tcgtggatta tgagttatcc aagtatcaat
     5101 ttattgtttc tgaaccagta caataatcgg tataaagatt tggtagaaac tatcggggac
     5161 cttatttacg aaaaattgga cacaagaatt tataattatc tgaaacaacg tgttgctatt
     5221 tccgattccg gttatgtaaa tatcccacat cgacaaattg cagaagaaat tgcctccagc
     5281 agagaagttg ttacccgctt gttaaaaaaa ttggaagccg acaaaaaaat tgtccaaacc
     5341 cgcgatggga taaaagtcct ctaacgatgt gaccaatatc acaaaaaaca agctgaccca
     5401 cgttctactt ttgtatcaac atttaggcaa tggcaaagaa atttatttta gtttgtatat
     5461 cctgtattgc tattgcggca ttaattgctg cttgtatcca attattcaaa cgttaaaact
     5521 atttattatg aaaaagaaca tgggctcaac cgaccgcatc ctgcgcataa tcattgctgc
     5581 agtatttgca attctctatt ttaccggaac tgtatcagga acattaggac tggtgttatt
     5641 aattctcgga gcagtatttg tgctgacttc cctgatttca ttctgtccgc tatattccat
     5701 ctttggttta aatacctgcc cggctaaaaa ataatttttt ccttttaacc ggttataaac
     5761 tgttaaacag atatgctgta cctgtttaac agttttttta ttcaatacac ttaaacagct
     5821 tttcaacgtg ttgttgattg caacgtattt attttaccta tctttgattg tacaaatcaa
     5881 aaatatgtgt agatacatct ttactcctct ttttttagtc gctgtccttt ttacaacaac
     5941 agtcagcgcg cagtttaaca ttgactttct gggtcagtta gaatatgacg atcagttgtc
     6001 caaccttaca ggctggtctg atggggcagg tcatgaatac gcaatagttg gtaccgaaga
     6061 cggaacttcg atagtagata ttaccgaccc taccaacccg gttgaagtgc agtttgtgga
     6121 tggaaataac agcatttggc gtgaagtaag aacctggagc cattatgcat atgtggttac
     6181 cgaagcaggt ggtggattac tttgtatcga tttatctaca ttgcccggac cggttgactt
     6241 caactttaca gatggtggtg ttggattaaa ctcaggacat actgtttttt gtgatgaaaa
     6301 tggttatgtg cacgtttttg gctctaataa aggtgttggt ggtgatcaaa tttttaatgc
     6361 caatatcgac cctatggacc ctccttttgc cggcgaggtg gaagaatggt atatccatga
     6421 cggatttgta agaggcgata ctttatgggc aggcaatata tatgatggct ggttttctgt
     6481 ttgggatatc agcgacaaaa catcacctga gttaatggcg cagcaaagca ctccggacaa
     6541 ttttacacat aacgtttggc ttacagatga taataacttt ttattttcaa ccgatgaagt
     6601 aaccaacgcc gttgtagctt cttacgatgt ttcagacctt accgatatca aagaattaga
     6661 ccagtttcgc gctaatcccg gcacaaactc tatacctcat aatacatttg tgcgtggcga
     6721 cttcctcgta acggcatatt accgcgatgg cgttgt
//
LOCUS       DAORKW010000153         6735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_382_length_6735_cov_5.401048, whole genome shotgun sequence.
ACCESSION   DAORKW010000153 DAORKW010000000
VERSION     DAORKW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_382_length_6735_cov_5.401048"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLB46_07505"
     CDS             <1..711
                     /locus_tag="PLB46_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76402.1"
                     /translation="VTTTENPDGTGAFYKPRFSNLGLSYSRKFADYLSGGLTVRVISE
                     SISDVKAIGVAMDIGIQYITGSDAHPEQIKMGITLRNVGTPMKFSGDGLSFRGLVPSG
                     DYSMTQDFRSETFELPSLLAIGGSYDFYFGLAHRLTMVGNFTSNSFYKDQFGAGLEYA
                     LKVKNTEMFMVRGAYRYESGITSDVDRTSASTGIAAGVTFQYPIDELGQNLVALNYSY
                     RTSYQYEGSHCFGLRLSF"
     gene            790..1263
                     /gene="greA"
                     /locus_tag="PLB46_07510"
     CDS             790..1263
                     /gene="greA"
                     /locus_tag="PLB46_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRF76403.1"
                     /translation="MADTIYLSKEGMENLKVEIQELKTKGRAEMSRQIKEAREKGDLS
                     ENAEYDAAKEAQGLMEMKIAKLEELLMNARLLDESKIDTSKVSVFTTVKLHDTKFKKD
                     IKYTLVSEKEADIKTGKISVTSPIGKGLLGKTVGETVKITVPAGVIEFKILEITV"
     gene            1266..1661
                     /locus_tag="PLB46_07515"
     CDS             1266..1661
                     /locus_tag="PLB46_07515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRF76404.1"
                     /translation="MPTIFSRIISGEIPCHKIAENETYFAFLDIMPLAPGHVLVVPKI
                     PVDYIFDVDDEILAGMLPFAKRIAHAMEKVIPCERIGVSVIGLEVPHAHIHLIPLRTM
                     DDINFSRPKLKMSNTELAEIAAKIVSHLQ"
     gene            complement(1663..2226)
                     /gene="ruvC"
                     /locus_tag="PLB46_07520"
     CDS             complement(1663..2226)
                     /gene="ruvC"
                     /locus_tag="PLB46_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRF76405.1"
                     /translation="MSVNSKQQIIMGVDPGTNILGYSIICSDKNTIKILINGVIQLKK
                     TDDHFVRLGIIYTRLCALIKEYQPTAMAIEAPFFGKNVQSMLKLGRAQGAAIVAGASN
                     NIEVIEYSPRRIKQSITGRGNASKEQVAKMLQSTLQLTSLPKYFDETDALAVAVCHIN
                     ASKKIAVTGKQFKNWSDFIQANPGKVK"
     gene            2238..3218
                     /locus_tag="PLB46_07525"
     CDS             2238..3218
                     /locus_tag="PLB46_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HRF76406.1"
                     /translation="MKSRNNKTIFWSVAIKVTVFLLLLFALYKQLYLNEEAQTIPDKL
                     LNVGTGELIGYLLLLTILMFLNWGIEAYKWQQLVVKITPIRFFRTFKAVWTGVTLGLF
                     TPNRVGEFGGRILYVPRKYRIKAVIVSLIGSFSQNLATIIIGIIGLIIYLHQVEQITL
                     SVTFAVGLVSAIAITLLLLAYYNLDVVVQLFKRSKYLKRIYPYTAILAEYHSRDLTKL
                     LLLAFWRYAVYTAQYLIFLKMFGAEINIVSGISAIGVIYLAQTVIPSFAVVELLTRLP
                     VATLIFSKYGIPIGTSLAATTSIWMLNLILPAILGYIFIIRYNFFKNRQS"
     gene            3215..4063
                     /locus_tag="PLB46_07530"
     CDS             3215..4063
                     /locus_tag="PLB46_07530"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRF76407.1"
                     /translation="MIKPLLITSLSFGTLLSGNVEETMPSRIMPASETCEIPHMAFQA
                     GESLTYKVYYNWGTVWLSGGEVYFKLTKEDYKGKPVLHASGEAVSYKSFDWLFKVRDK
                     VDTYMNEETLQSYKFSRDVYEGGYTFYRSYDWDREKNIIYSYQDNRKGKKTSKTITNV
                     DPCGVDLMSTFYWMRTLDIASAKKGDKIPVKMAIDDKEYDMYVRYEGKEVYETKLGKF
                     NCIKLKPLLQEGEIFKEGEGMTLWLTDDDNRIPVRIESGLRVGRITCDLKAYGGNKYP
                     FTSKVN"
     gene            complement(4113..5033)
                     /locus_tag="PLB46_07535"
     CDS             complement(4113..5033)
                     /locus_tag="PLB46_07535"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HRF76408.1"
                     /translation="MRKNNDNMLNRMALHKGDKVGVLSTARWIDEQELEEANELLRNW
                     GLIPVYGNSIGAKFNQFAGEDSLRAENLQTFLDDPEIKAIICARGGYGTIRIIDRIDF
                     TGFMANPKFICGYSDITVLHAHINDKLGLPTIHSTMPFSFSRNSILAKQTLQNALFGN
                     ALNIQAEPHPLNNFGTAEGVLIGGNLSILYSLLGTRFGFSTAHKILFIEDIDEYLYHI
                     DRMMISLKLAGKLDAVKAIIVGGMTDMKDNKVKFGTSAEEIIATHVQPYNIPVCYGFP
                     AGHIDDNRAMVFGRKIQLEITAEGTLLSYK"
     gene            5049..>6735
                     /gene="metG"
                     /locus_tag="PLB46_07540"
     CDS             5049..>6735
                     /gene="metG"
                     /locus_tag="PLB46_07540"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRF76409.1"
                     /translation="MQVPERYLITSALPYANGPLHVGHIAGAYLPADIYVRYLRKQKR
                     DVLFICGSDEHGAAITIQAKKENTTPQAIIDKYHKVIEDAFKGLGISFDIYHRTSSPI
                     HHETSQEFFLKLYNNQVFEEKESEQYYDEAYNQFLADRYIMGTCPVCANPNAYGDQCE
                     KCGTSLSPNDLINPVSTLSNQPPVKKATKHWYLPLNKFQNWLNDWIIKGEGQTEEWKK
                     NVLGQCASWINNGLHPRAITRDLDWGVKVPLPGAEGKVLYVWLDAPIGYISATKQWSL
                     NTGKDWEPYWKSNSSKLVHFIGKDNIVFHCLIFPCILKSHGDYILPTNIPANEFMNME
                     GDKMSTSRNWSVQLHTYLEEFPGKEDVMRYVLCANMPETKDSEFTWKDFQARNNNELV
                     AILGNYANRVMVLTAKYNDGKVPEVNQALLSEPYVVTYQAQIKALIEEVYCPAIENYR
                     FRDALSAMMDIVRLGNKLLTDYEPWKLIKTDEAKTLGILRLCLESLVDIAIICEPFLP
                     FSAQKILNYFNLNDPGFDYLSLKLSTGAALGSPFYLFEKVEDEVIEKKVAALEE"
BASE COUNT         2112 a         1192 c         1316 g         2115 t
ORIGIN      
        1 gtaactacta ctgaaaatcc tgacggaaca ggtgcttttt acaagcctcg tttttctaac
       61 cttggattgt cttattcgcg taagtttgcc gactatttaa gcggtggttt aaccgtgcgc
      121 gttatttctg agagcatttc agacgtaaag gcgattggtg ttgccatgga tattggtatt
      181 caatatatta ccggttctga tgcacaccct gaacaaatca aaatgggtat taccttaaga
      241 aatgttggaa caccaatgaa gttttcaggc gatggtctta gcttcagagg tttagtgcca
      301 tcaggagatt attcaatgac tcaggacttc cgttctgaaa cattcgaatt gccttcattg
      361 ctagctatcg gtggttctta cgacttttat ttcggattag cacacagact aactatggtg
      421 ggtaacttta cttccaactc attctataaa gaccaatttg gtgcaggttt agaatatgct
      481 ttgaaagtaa aaaatactga aatgtttatg gttcgcggtg cataccgtta cgaatcaggt
      541 atcaccagtg atgttgaccg caccagtgct tcaacaggta tcgctgccgg tgttactttc
      601 cagtatccaa tcgacgaatt aggtcaaaat ttagtagcac ttaattactc ttacagaaca
      661 tcatatcagt atgaaggctc acattgtttt ggccttcgtc tgagcttctg ataaaattct
      721 tttataactt tgcggaaacg tttctgaaca aggaacgttt cttttttttt gtattttaaa
      781 ctttatcgta tggctgatac tatttatctg agtaaagagg gaatggaaaa ccttaaggtg
      841 gaaatccagg aattgaaaac gaagggtcgt gctgaaatgt cacgtcaaat taaagaagca
      901 cgcgaaaagg gtgacttgtc agaaaacgct gaatatgatg cagcaaaaga ggctcaaggt
      961 ttaatggaga tgaaaatagc caaacttgag gaattgctca tgaatgccag acttttagat
     1021 gagtcgaaaa ttgatacttc aaaagtgtcg gtatttacaa ctgtaaaatt gcatgatacc
     1081 aaatttaaaa aggatataaa atacaccttg gtatctgaaa aggaagcaga tatcaaaacc
     1141 ggaaaaattt cagttacttc tccaattggt aaaggattgt taggtaaaac cgtaggagaa
     1201 actgtaaaga ttactgttcc tgcaggtgta attgaattta aaattcttga aataaccgtt
     1261 taattatgcc aacaattttt agccggatta tcagtgggga aataccatgt cataaaattg
     1321 ctgaaaacga aacctatttt gcgtttttag atatcatgcc attggctccc ggtcacgttt
     1381 tagttgtgcc taaaattccg gttgattata tttttgatgt agatgatgaa atattagctg
     1441 gtatgttacc atttgcaaaa cgaattgcac atgccatgga aaaagtaata ccgtgcgaac
     1501 gtattggtgt ttctgttatt ggattagaag tacctcacgc gcatattcat ttaatacctt
     1561 tacgcacaat ggacgatatc aattttagtc gcccaaaact taaaatgtct aataccgaat
     1621 tagctgaaat agctgcgaaa attgtcagcc acttacaata acttatttca cttttcccgg
     1681 atttgcctga ataaaatcgc tccagttttt aaattgtttt ccggttactg caattttttt
     1741 cgaagcatta atatgacaaa ctgctacggc taatgcatca gtttcatcaa aatatttagg
     1801 caatgacgta agctgtaatg ttgattgcaa catttttgcc acctgctctt tactggcatt
     1861 tcctcgtcct gttatactct gttttatcct gcggggactg tactcaatta cttcaatatt
     1921 attacttgca ccggctacaa tagccgcccc ctgtgcacgc ccaagtttaa gcatagattg
     1981 cacatttttt ccaaaaaagg gcgcttcaat tgccattgcc gttggctgat attctttgat
     2041 taacgcacat aaccgcgtgt aaataatgcc aagccgaaca aaatggtcat ctgttttttt
     2101 taactgaatt actccgttaa tcaatatttt gatagtattt ttatccgaac atataatact
     2161 gtaacccaat atatttgttc cggggtcaac acccattatt atttgttgct tagaattaac
     2221 cgacattttt ttaccaattg aaaagccgaa ataacaaaac gatattctgg tctgttgcca
     2281 taaaagtaac ggtttttcta ttattacttt ttgcgcttta taaacaattg tatcttaatg
     2341 aagaagcgca aactataccg gataaattac taaatgttgg cactggtgag ttgattggat
     2401 atttattact gctaactatc ctcatgtttt taaactgggg catagaagcg tacaaatggc
     2461 aacaattagt tgtaaaaatt acacctatcc gcttttttag aacgtttaag gctgtgtgga
     2521 caggtgtgac attgggattg tttacaccaa acagggtagg tgaatttggc ggtagaatat
     2581 tatacgttcc gcggaagtat agaataaagg cagttattgt ttcgcttatt ggtagcttca
     2641 gccaaaatct agctactatc attattggaa ttattggact aattatctat ctccatcagg
     2701 tagaacaaat tacgctttct gtaacctttg cagtgggatt ggttagcgca attgccatta
     2761 ccttgttatt gctggcttat tataacctgg acgttgttgt gcagctgttt aagcggagca
     2821 aatacctgaa acgtatttat ccctacacgg ctattcttgc ggaatatcat tctcgtgact
     2881 taacaaagct actcctgctt gcgttttggc gatatgcagt atatacggca cagtatttga
     2941 ttttcttaaa aatgtttggg gcagaaataa atattgtgag cgggatttca gctatcggcg
     3001 ttatttatct ggcccaaaca gtaataccaa gttttgcagt tgttgaactg ttgacacgcc
     3061 tgccggttgc cacattaatt ttttcaaaat atggcatacc aattggaaca tcactggcag
     3121 ctacaacaag tatatggatg ctcaatttga tattaccggc aattttggga tatatattta
     3181 taattagata taattttttt aaaaacagac aatcatgata aaaccattgt taattacgtc
     3241 gctttcattt ggtacgttat tatccggaaa tgtagaagaa acgatgccgt cgcgaataat
     3301 gccggcaagt gaaacctgcg aaattcctca tatggctttt caggcaggtg aatcattaac
     3361 gtataaagtt tattataact ggggaactgt atggttaagt ggtggtgagg tgtattttaa
     3421 acttactaag gaagattata aagggaaacc tgttttacat gcttcaggtg aagcggtaag
     3481 ttacaaatcg ttcgactggt tatttaaagt tcgcgataaa gtggatactt atatgaacga
     3541 agaaacattg cagtcctata aatttagccg cgatgtatat gaaggtggtt atacctttta
     3601 tcgctcttat gattgggaca gagaaaaaaa tattatttat tcttatcagg ataacagaaa
     3661 aggtaaaaaa acttcaaaaa caattaccaa tgttgatcca tgtggggtag atttaatgtc
     3721 aactttttat tggatgcgta cacttgatat tgccagtgct aagaaaggag ataaaattcc
     3781 tgtaaaaatg gctattgatg ataaagaata tgatatgtat gtgcgatatg aaggcaaaga
     3841 agtgtatgaa actaaactcg gtaaatttaa ttgtattaaa ttaaaaccat tattacagga
     3901 aggtgaaata tttaaagaag gtgaggggat gacattgtgg ttaactgacg atgataaccg
     3961 cattccggtg cgcatagagt ctggattgag agtaggaaga ataacctgtg acctaaaagc
     4021 atacggagga aataaatatc cgtttacttc taaagtgaat taaattctgt cgtgattata
     4081 tgtaaaggtt catcagtatt ggtgaacctt ttttatttgt aacttaataa tgtaccttcc
     4141 gcagtaattt ctaattgaat cttccttcca aataccatgg cgcgattatc gtcaatatgc
     4201 cctgcaggga atccataaca taccggaata ttataaggtt gaacatgtgt tgcaattatt
     4261 tcttccgcac tcgtaccgaa ttttacttta ttatctttca tgtctgtcat cccaccaaca
     4321 ataattgctt taactgcatc taattttcct gctaatttaa gactaatcat catgcggtcg
     4381 atatgatata agtattcatc aatatcttca ataaataaaa ttttatgcgc tgttgaaaat
     4441 ccaaagcggg tgcccaataa tgaatataaa atagataggt tacctccaat taatacccct
     4501 tctgcggtac caaaattatt taatggatga ggttcagctt gaatatttaa tgcattacca
     4561 aataaagcat tttgtaaagt ttgtttcgcc agtatactat ttctggaaaa tgaaaatggc
     4621 atggtggagt gaatagtcgg caatcctaat ttatcgttga tatgtgcatg taaaacagtg
     4681 atgtcagaat aaccacaaat aaatttagga ttagccataa atcctgtaaa atcgatgcgg
     4741 tcaataatac gaatagtacc ataaccacca cgtgcacaaa taatggcctt aatttcaggg
     4801 tcatctaaaa aagtctgaag gttttcagcc ctcaaactat cttctccggc aaactggtta
     4861 aattttgctc caatcgaatt tccataaacc ggaattaagc cccaattgcg cagtaattcg
     4921 tttgcttctt ctagttcttg ttcatcaatc caacgcgctg ttgaaagcac tccaacttta
     4981 tctcctttat gtaatgccat acgattgagc atattatcat tatttttgcg cataaaattg
     5041 agaaaaactt gcaagtacct gaaagatatt taataacatc tgcccttcca tatgctaacg
     5101 gccctttgca tgttggccat attgccggcg catatttacc agccgacatc tatgtgcgtt
     5161 atctgcgcaa acaaaaacgc gacgtattat ttatttgtgg gagcgatgag catggtgctg
     5221 caattactat tcaggcaaaa aaagaaaata ccacccctca ggcaattatt gataaatatc
     5281 acaaggttat tgaagatgca tttaaaggac tggggattag ttttgatatt tatcaccgaa
     5341 catcatctcc cattcaccat gaaacgtcac aggagttttt tctgaaactc tataacaacc
     5401 aggtgtttga ggagaaggaa agcgaacagt attacgacga agcctacaat caatttttgg
     5461 ccgaccgata tattatggga acatgcccgg tatgtgcaaa cccaaatgca tatggcgacc
     5521 agtgtgaaaa atgtggaacg tcattatctc ccaacgattt gataaacccg gtttcaacgt
     5581 taagtaacca gcctcctgtt aaaaaagcaa ccaaacattg gtatctgcca ttaaataaat
     5641 ttcaaaattg gctaaacgac tggattatta aaggtgaagg ccaaactgaa gaatggaaaa
     5701 aaaatgtgtt gggacaatgt gcaagctgga taaataacgg attacatcca cgcgccatta
     5761 cccgcgattt ggattgggga gtaaaggtgc cacttccagg cgctgaaggg aaagtgctgt
     5821 atgtttggtt ggatgcacct attggatata tcagtgctac caaacaatgg tcgcttaata
     5881 ccggaaaaga ttgggagcca tactggaagt caaattccag caaacttgtt cattttattg
     5941 gaaaggacaa tattgttttt cactgcctca ttttcccttg tattttaaaa agccatggcg
     6001 actatattct acctaccaat attccggcta atgagtttat gaatatggaa ggcgataaaa
     6061 tgtctacttc ccgcaattgg agtgttcaat tgcataccta tttggaggaa ttcccgggca
     6121 aagaagatgt gatgcgttat gtgttatgcg ctaatatgcc cgaaaccaag gacagtgaat
     6181 ttacctggaa agattttcag gcgagaaata ataatgagct tgttgcaatt ttaggaaatt
     6241 atgccaacag ggtaatggtg cttaccgcta aatataacga tggcaaagta cccgaagtta
     6301 accaagcgtt gcttagcgag ccttatgtgg tgacttatca ggctcaaatc aaggctttga
     6361 ttgaagaagt ttattgccct gcaattgaaa actatcgctt tagagatgcg ctttcggcaa
     6421 tgatggacat tgttcgcctg ggcaataagc tgcttaccga ttatgaacca tggaaactca
     6481 tcaaaaccga tgaagctaaa acccttggaa tactcaggtt atgccttgaa agtctggtag
     6541 atatcgcaat tatttgcgaa ccatttttac ctttttcagc ccaaaaaatc ttgaactatt
     6601 tcaacttaaa tgacccgggt tttgactatt tgtcgttgaa actttctaca ggtgcagctt
     6661 tagggtcacc tttttattta ttcgaaaagg ttgaggatga ggtaattgaa aaaaaagtag
     6721 cagcattgga agaaa
//
LOCUS       DAORKW010000154         6670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_390_length_6670_cov_6.784278, whole genome shotgun sequence.
ACCESSION   DAORKW010000154 DAORKW010000000
VERSION     DAORKW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_390_length_6670_cov_6.784278"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLB46_07545"
     CDS             <1..331
                     /locus_tag="PLB46_07545"
                     /inference="COORDINATES: protein motif:HMM:NF017830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76410.1"
                     /translation="GDITVAIQEDGKAREIVIKEGDMFLLPPNVPHSPRRPANTIGLV
                     IERYRTEKEFDSCMWFCEKCNHKLHEEKFPLKDIVNQLPAIMNSFYDNLDLRTCDNCG
                     HVMEPPK"
     gene            848..1231
                     /locus_tag="PLB46_07550"
     CDS             848..1231
                     /locus_tag="PLB46_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRF76411.1"
                     /translation="MVELNLLHKDLTDLIIQAFYKVYNELGYGFLEKVYENALIIELK
                     KIGLLIEQQVKIKVYYDETPVGFYLADLLVESSVIVEIKAAESLCYEHECQLINYLKA
                     SNVEVGLLLNFGKKPTFKRKVFSKK"
     gene            1277..2290
                     /locus_tag="PLB46_07555"
     CDS             1277..2290
                     /locus_tag="PLB46_07555"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF76412.1"
                     /translation="MLKIDIHTHIIPDQLPKWSEKFLDNRYIHLDHHCPGCARMMQGD
                     KFFREIQSNCWDGEVRIHDCDATGVDVQVLSAIPVMFHYWEKNAAYALETSRFQNDFI
                     AEVVSRHPKRFVGLGTVPLQDPELSIQEMTRCVKELGLAGIEIGSHVNDWNLNAEELF
                     PFFKAASELNAAIFVHPWDMMGKDKMNKYWLPWLVGMPAETSLAICSMIFGGVFERLP
                     NLKVAFAHGGGSFPATIGRIEHGFNVRPDLVAIDNKENPRNYLNKFYLDTLVHDEMMM
                     RYILDLFGPKQLALGSDYPFPLGEHHPGKLIESMQLSNEVQERLLNGTALEWLGLDGK
                     LFT"
     gene            complement(2630..4165)
                     /locus_tag="PLB46_07560"
     CDS             complement(2630..4165)
                     /locus_tag="PLB46_07560"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRF76413.1"
                     /translation="MRIEKFQYIREFSQYENREVTIKGWVSNKRDSKGLVFIIMRDGS
                     GFTQCVADAAKLSAEQFEAAKRLTLESSLTLTGTVVKDERQIGGYELQITNLEIIQVA
                     EEYPISKKEHGVEFLVDNRHLWLRSKKQWAMMRVRNRVIFAIHNFFQQNDFLQMDAPI
                     FTGNACEGTSTLFETDFYGRPAYLSQSGQLYGEALAMAHGKIYTFGPTFRAEKSKTRR
                     HLSEFWMIEPEMAFYDLDMNMDLIEQFLKAVVKDVTTFCKEELEILERNTAFLENAIN
                     LKFPRLHYDDAVAIIRGEKDVDGKNSIQTLENDLKEINDKITSLQNEITERENKIAGG
                     TLKAGEINFNKNKIDTIKNEIKDLQEKADNIPQWLNSARNFEHGNDFGGSDETVLTRL
                     FACPIMVYNWPTAVKAFYMKRDPNDPRYVKGVDVLAPEGYGEIVGGAEREDNYEMLLE
                     RIKHEELPVEAFDWYLDLRKYGTVPHAGFGLGLERMVLWISGATHIREAIPFPRFYGR
                     LEP"
     gene            4327..5814
                     /gene="rpoN"
                     /locus_tag="PLB46_07565"
     CDS             4327..5814
                     /gene="rpoN"
                     /locus_tag="PLB46_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRF76414.1"
                     /translation="MLNQRLSQKLLQKLSPQQIQLMKLLQVPTDQLEQRIKEELEANP
                     ALEEGDNGEEELTSTEEIFREEEGNNEESSQENEESNSSDELDLDDYINDDDVADYKT
                     RDDSYGDDDEDNKTVPYAVSDTFHENLTKQLGMCELDERYHKIAEQIIGSIDDDGYLR
                     REISAIVDDLAFSQNVMTEENEIIELLDLIQGFDPPGVGARDLRECLLLQLERKTQTK
                     HIKLAINIIENYFDEFTKKHYDKLARHLKVEDDVLKPVIAEITKLNPKPGGSAKEDGS
                     NLYQYIIPDFTIVNSNGDLELKLNSRNAPELRVSRDFRDMMVDYKNSSSKDKKQKEAI
                     MFIKQKIDAAKWFIDAIKQRQHTLWSTMYEIMLHQYDFFLTGDETMMKPMILKDIAEK
                     TNLDISTVSRVANSKYVQTEFGTYALKYFFSESLSTDTGEEVSTREVKKILSELIEME
                     DKKSPLSDQELMEDLRKRGYNIARRTVAKYREQLDIPVARLRREL"
     gene            5811..6428
                     /locus_tag="PLB46_07570"
     CDS             5811..6428
                     /locus_tag="PLB46_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76415.1"
                     /translation="MIKKIFGHVFSIVFLPLLLPLYGTIYVIYTNPYTFPDPPSYALT
                     VIRVALLTFVFPAFTIGLLAALKFIKSVNVYDRQERIIPYIAAGFFYIWAYVVFLKEG
                     FNPQITFILLGSTIAVFVDFLINILFTKVSMHTTGAGGMIAVIMILFPYSYVGILPVF
                     LITIVCAGCVGAARLALKAHTDREVYLGYMVGFISFMVAANFFQA"
     gene            complement(6409..>6670)
                     /locus_tag="PLB46_07575"
     CDS             complement(6409..>6670)
                     /locus_tag="PLB46_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRF76416.1"
                     /translation="IFFVAGLGRNSFDNKYQFAIVMAPSAIVKSEPSENSTNLFLLHE
                     GIKLKLMRNDEGWTEIKIPNGEIGWIKSDEITAVDPFMPGKN"
BASE COUNT         2107 a         1178 c         1334 g         2051 t
ORIGIN      
        1 aggtgatatc actgttgcaa tacaggaaga tggtaaagca agagaaatag tgattaaaga
       61 aggtgatatg tttttattgc caccgaatgt gccgcattct ccgcgtcgcc cggctaatac
      121 aattggatta gtaattgagc gttaccgcac tgaaaaagaa tttgattcat gtatgtggtt
      181 ttgcgaaaaa tgtaatcaca aattacatga agaaaaattt ccattaaaag atattgtaaa
      241 tcaattgccg gcaattatga attcctttta cgataacctc gacctccgca cctgcgataa
      301 ctgcgggcac gtgatggaac cgccaaaatg aggaacacgg atgacgcgga tgacacggat
      361 taacgcggat ttaaaagggg tggtgttgaa tattgaaata attgaacgcg gatgacactg
      421 atgacgcgga ttaacgcgga tgtgaattgg atggtgttgt atattgaaat aatggaacgc
      481 ggatgaagca gatgacacga attaacgcgg atttaaaagg gatgatgttg tatattgaaa
      541 taatagaacg cgaatgacga ggatgaacgc ggatgctgat gggatggtgt tgtatattga
      601 aataatggaa cgctgatgtc gcggattatc gcggatgtta atgggatcgt gttgtatatt
      661 gaaataatag aatgttgatg acgcggatta tcgcggatgt taatgggatc gtgttgaata
      721 ctgaagtaat ggaatatgtt tgacacaaat gttttaaaat gtttcatgat aatatttgaa
      781 ttgaaaagtt tatcctaaga ggtaaacaca ctttatagta ttgtgaataa aattgtgatg
      841 ttgtgatatg gtggaattga atttattaca caaagattta actgatttaa taattcaggc
      901 attttacaaa gtttataatg aactcggtta tgggttcctt gaaaaggtct atgaaaatgc
      961 actcataatt gaattaaaaa aaataggttt acttattgaa cagcaagtaa aaattaaagt
     1021 ttattatgat gaaacgccag ttggatttta tcttgcagat ttactagttg aatctagtgt
     1081 cattgtcgaa attaaggctg ccgaatcctt gtgttatgaa catgaatgcc aattaattaa
     1141 ctaccttaaa gcatcaaatg ttgaagttgg tttgttatta aatttcggga aaaaaccaac
     1201 tttcaagaga aaagtatttt caaaaaaata aaaccaaatc cgcgttcatc cgcgtcatca
     1261 gtgtcatctg cgttctatgc ttaaaatcga tattcacacc catatcatcc cagaccagct
     1321 cccgaagtgg agcgagaagt ttttggataa cagatatatt catttggatc atcattgtcc
     1381 gggttgtgcg cggatgatgc agggggataa attttttaga gaaattcagt ctaattgctg
     1441 ggatggagaa gtgcgcatac acgattgcga tgcaacaggt gttgacgtgc aggtattaag
     1501 tgctattcct gtaatgtttc attattggga aaaaaatgct gcctatgcac tagaaacttc
     1561 ccgctttcaa aatgatttta ttgcagaagt agtttctcga catccaaaac gttttgtagg
     1621 attgggcact gttcctttac aagaccctga gttgagtatt caggagatga cgcgttgtgt
     1681 aaaagaatta ggattagcag gtattgaaat tggttcgcat gttaacgact ggaatttaaa
     1741 tgcggaagaa ttatttccgt tttttaaagc ggcttcagaa ttaaatgctg ccatttttgt
     1801 gcatccgtgg gatatgatgg gtaaggataa aatgaataaa tattggttgc cttggctggt
     1861 gggtatgcct gcagaaactt ctttggcaat ttgttctatg atttttggtg gtgtatttga
     1921 gcgtttgcca aatttaaaag tggcatttgc acatggtggt ggaagttttc ctgctactat
     1981 cggacgtatt gaacatggat ttaatgttcg tcccgattta gtagctattg ataacaaaga
     2041 aaatccaaga aattatctca ataaatttta tttggacact ctagtgcatg atgaaatgat
     2101 gatgcgatat attttggatt tatttggtcc aaaacaatta gcattaggaa gtgattatcc
     2161 gtttccatta ggcgaacatc atccggggaa attaattgag tcgatgcaat taagtaatga
     2221 agtgcaagag cgattgttaa atggaactgc gctggaatgg cttggattgg atgggaaatt
     2281 atttacgtga gttttaacat ccgaagcttc aaagggctcc tgttctgcaa aatctctgat
     2341 tctgcagcat cgtgccagga cgctgtctaa cgcgctccat aaataaaaac aactattatg
     2401 gttttacttt gcaaattata ttgtgtgtta aaacagccga aaccacagag aaaacccaaa
     2461 gaaaacagcc cgcagagctc acagagaagt ttatactaat gaattaggaa ccaaaaccac
     2521 aattattaat gcaaagattt ttaacagagg atatacaaag aaagcaataa aaaaaaccgg
     2581 agagaattat aaaaattcgt tccggttttt ttgtgatgat tatttttatt tatggttcta
     2641 agcgaccata aaaacgtgga aacggaattg cttcacgaat atgcgttgcg ccgctaatcc
     2701 ataacaccat acgttctaat cccaaaccaa atccggcatg cggaacagtt ccgtatttac
     2761 gcaaatctaa ataccagtcg aaagcttcta caggtaattc ttcgtgttta atgcgctcca
     2821 ataacatttc ataattgtct tcgcgttcgg caccaccaac aatttcaccg tatccttccg
     2881 gcgctaacac atcgacacct tttacgtaac gcggatcgtt tggatcgcgc ttcatataaa
     2941 atgcttttac agcagtcggc caattgtaca ccattattgg gcaagcaaac aatcgagtta
     3001 aaactgtttc atcacttcca ccaaaatcgt taccatgttc aaagttgcga gcagagttta
     3061 accattgcgg aatattatct gctttttcct gcaaatcttt tatttcattt ttaatggtgt
     3121 caattttatt tttattaaaa ttaatttcac ctgcttttaa agtcccacct gcaattttat
     3181 tttcacgttc ggtgatttcg ttttgcagtg aagtaatttt atcattaatt tcttttaaat
     3241 cattttccag tgtttgaata ctgttttttc catctacatc tttttcgccg cgaataatgg
     3301 caacagcatc gtcataatga aggcgcggaa atttcaggtt aatggcgttt tcgagaaaag
     3361 cagtatttct ttccaaaatt tccaattctt ctttacaaaa tgtagtaaca tcttttacaa
     3421 cagcctttaa gaactgttca atcaaatcca tattcatatc taaatcgtaa aacgccattt
     3481 ccggttcaat catccagaat tcggaaaggt gtcggcgcgt tttagatttt tcggcgcgga
     3541 atgtagggcc aaaggtgtaa attttaccat gtgccattgc caatgcttcg ccgtataact
     3601 gaccactttg gctcaggtat gcagggcgac catagaagtc ggtttcaaac aaagttgaag
     3661 ttccttcaca ggcattgccg gtaaaaatcg gggcgtccat ctgcaaaaag tcgttttgct
     3721 gaaagaaatt atggatggca aaaattacgc ggtttctcac gcgcatcata gcccactgtt
     3781 ttttgcttct cagccataaa tggcgattat ctaccaaaaa ctcaacaccg tgttcttttt
     3841 ttgatatcgg atattcttcg gcaacctgaa taatttcaag gttggtaatc tgtaattcgt
     3901 atccaccaat ctggcgctca tctttaacca ctgttcctgt aagtgttaat gagctttcga
     3961 gcgtaaggcg ttttgcggcc tcaaactgct cagcactcaa ttttgcagca tcggcaacac
     4021 attgtgtaaa cccgctgccg tcgcgcataa tgataaatac caggccttta ctgtcacgtt
     4081 tattactcac ccaacccttg atggttactt cacggttttc atactgactg aattctctga
     4141 tgtattgaaa tttttcaatt cgcatgctaa atatgattaa gggtgcaaaa ttacaatgaa
     4201 ttttctgtga aatccaccca ctgtacagat ttcattaata aataaaaact taacctacaa
     4261 gagaaaattt attgctgtca caaataaatt cgacttaaat ttggcgcagg ataggattaa
     4321 aatacaatgt taaatcagcg tttaagccaa aaattattac aaaagctgtc gccacagcag
     4381 attcagctca tgaagctgct gcaggtgcca accgaccagc tggagcagcg catcaaggaa
     4441 gaacttgagg ctaatccggc attggaagaa ggcgataatg gggaagagga gctcaccagc
     4501 acagaagaaa ttttcagaga agaagaaggc aataacgagg agtcatccca ggaaaatgaa
     4561 gaaagtaaca gcagcgatga gctggattta gacgattata ttaacgacga tgatgttgcg
     4621 gattacaaaa cccgtgacga tagttatggt gatgatgacg aggacaacaa aacagttcct
     4681 tacgccgtta gcgatacatt tcatgaaaat ttgaccaaac aattaggtat gtgcgaactc
     4741 gatgagcgct accataaaat tgcagaacaa attattggca gtatcgacga cgatggatac
     4801 ttacgcagag aaatttcggc tattgttgat gatttggcgt tttcacaaaa tgtgatgacg
     4861 gaagaaaatg agattatcga attattggat ttaattcagg gttttgaccc tcccggagtt
     4921 ggcgctcgtg atttacgcga atgtttattg ttacagttag aacgcaaaac gcaaactaaa
     4981 cacattaaac ttgccattaa cattatcgaa aattatttcg acgagtttac taaaaaacat
     5041 tacgataaac ttgcccgtca cttaaaagtt gaagacgatg tattgaaacc ggttattgcc
     5101 gaaattacca aactcaatcc aaaaccgggt ggaagtgcaa aagaagacgg tagcaattta
     5161 tatcaataca ttattcccga tttcaccatt gtaaactcca atggcgattt agaattaaaa
     5221 ctcaattccc gcaatgcacc tgaacttcgt gttagtcgcg acttcagaga tatgatggtg
     5281 gattataaaa acagctcatc aaaagataaa aaacaaaaag aagccatcat gttcatcaaa
     5341 caaaaaattg atgcagcaaa atggtttatc gatgctatta aacaacgcca acatacactg
     5401 tggagcacca tgtacgaaat catgttacac caatatgatt ttttcttaac aggtgatgaa
     5461 acaatgatga agccgatgat attaaaagat attgcagaaa aaaccaatct cgacatttca
     5521 acggtgagcc gtgttgcgaa tagtaaatat gtgcaaacag aatttggcac ctatgcctta
     5581 aaatattttt tcagcgagtc attatctacc gatacgggag aagaagtttc aactcgtgag
     5641 gttaaaaaaa tattgagcga actcatcgaa atggaggata aaaaatcgcc gcttagcgac
     5701 caggaattaa tggaagactt gcgcaaacgc ggttataata ttgcaagaag aacagttgct
     5761 aaatatcgcg agcaattaga tatacctgtt gcgcgtttaa gaagagaatt atgataaaaa
     5821 aaatcttcgg gcatgttttc tccattgtat ttctgccgtt attgctgccg ttatacggaa
     5881 caatatatgt aatatatact aatccttata ctttccccga tccaccttct tatgccctta
     5941 cggttattcg tgtggcctta ttaacatttg tgtttccggc ttttaccatc ggattacttg
     6001 ctgcattaaa atttattaaa tcggtaaatg tatacgacag gcaggaaaga ataattcctt
     6061 atatagcagc aggctttttt tatatatggg cttatgttgt ttttttgaag gagggattta
     6121 atccacaaat cacctttatt ttattaggct ccaccattgc ggtttttgtt gacttcttaa
     6181 tcaatattct atttaccaaa gtaagtatgc ataccacagg tgccggagga atgatagctg
     6241 taattatgat tttatttccc tattcgtatg tgggcatttt accagttttt ttaattacca
     6301 ttgtttgtgc cggctgcgtt ggtgcagctc gtttagcatt aaaagcacat accgaccgtg
     6361 aagtgtatct cggttatatg gttggtttta taagctttat ggttgctgct aattttttcc
     6421 aggcataaaa gggtcaactg ctgttatctc atctgattta atccagccaa tttctccatt
     6481 tggaattttt atttctgtcc agccttcatc gttccgcatt aattttaatt taatgccttc
     6541 atgcagtaag aataaattag tgctattttc tgagggttca ctttttacaa ttgcacttgg
     6601 tgccatcaca atagcaaact gatatttatt atcgaaacta tttctcccca aacctgctac
     6661 aaaaaatatt
//
LOCUS       DAORKW010000155         6637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_397_length_6637_cov_4.297934, whole genome shotgun sequence.
ACCESSION   DAORKW010000155 DAORKW010000000
VERSION     DAORKW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_397_length_6637_cov_4.297934"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..810
                     /locus_tag="PLB46_07580"
     CDS             88..810
                     /locus_tag="PLB46_07580"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76417.1"
                     /translation="MVRRYSQFLELVYFLVDILLLNISFLLAMLISFDRISKVTSDRK
                     FAMLLVAVNLIWFLVTSVFNYYKTERERRTGYEHLVFRFIKVLAFQALLTFTYLFLIK
                     GYQFSRSQLAYTYIFFTVIDLAWRIGFETYLKRYRARGGNYRRIVVVGTTNASLQFVK
                     EIQEHNEFGYRFMGYFDDPETSMAEVTGSLHQVKAFCIENNIDEIYFALQPSDMINYV
                     DDLMEFSNDKLIRFRIGPEFSN"
     gene            811..1491
                     /locus_tag="PLB46_07585"
     CDS             811..1491
                     /locus_tag="PLB46_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HRF76418.1"
                     /translation="MSKQFKKVNSEYYGNTPILSIRPEPLDNIYNRLQKRLFDIIFAL
                     LFFIIIGWWLFPIIALFVKLSSPGPVFFVQKRSGEGNEEFWCYKFRSMKVNAEADTKQ
                     ATVGDARITRVGSFLRKSNLDELPQFINVLLGDMSVVGPRPHMLKHTEEYSKIVNNFM
                     VRHFVKPGITGAAQANGYRGDTTDPVMMEKRVQYDLWYLENWSFWLDIKIVFLTVWSM
                     IKGNENAV"
     gene            1499..3100
                     /locus_tag="PLB46_07590"
     CDS             1499..3100
                     /locus_tag="PLB46_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76419.1"
                     /translation="MLLCCGALAVSAQQNYLPLNRNIYNWYEPALNKPGTGFHTAIRP
                     YRISDMDTVVTYDDVFHLKKVSGDGFFSKLGNWTAYDTILGVHKTEFHLRIDPIVNFG
                     GGLNLENGGQGVWNNTRGIGFSGDIGEKRNVSFNITLRENQGKYPDYVTAFVKEYNVV
                     PGQGKVRNFKGNAYDFANSSGYVSYNASKHFNFQLGHDKNFLGDGYRSMLLSDNAMFY
                     PFFKITTSVWKIKYMNLYTEFDDAYNGTIDSVVGVTRKFGSFHYLSWDVFPWVQLGLF
                     EGIVWPANDSTRYRGFDVNYINPIIFFRPIEFGLGSPDNAVIGANIKFTPLEKLIIYG
                     QFIMDDLDVAAARAGDGYYRNKFGIQGGFKLYEPFKIKNLIVQAEMNQANPYTYASKE
                     PVQNYAHYNQPLAHPLGANFREFIFIGNYRFFERFYLEAKLQSAVKGVDANNAEHNGG
                     DIFTSDFEIPGFPDSYGNFIGQGLKTTIKSTDLRAGYLLNPSINLNIEGQIFIRNYKN
                     DKDVLSTTMVMLSLKTDLFNAYYDF"
     assembly_gap    3286..3385
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3386..3457
                     /locus_tag="PLB46_07595"
     tRNA            3386..3457
                     /locus_tag="PLB46_07595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3419..3421,aa:Arg,seq:cct)
     assembly_gap    3715..3814
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4081..4155
                     /locus_tag="PLB46_07600"
     tRNA            4081..4155
                     /locus_tag="PLB46_07600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4114..4116,aa:Arg,seq:ccg)
     gene            4223..4459
                     /locus_tag="PLB46_07605"
     CDS             4223..4459
                     /locus_tag="PLB46_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRF76420.1"
                     /translation="MTVFVYALKNQINDEIYVGISADLDRRLKEHNSGKNRYTKAFIP
                     WSIFFSESCESYAEARKREIYYKSAAGKRKLKNR"
     gene            complement(4500..4889)
                     /locus_tag="PLB46_07610"
     CDS             complement(4500..4889)
                     /locus_tag="PLB46_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRF76421.1"
                     /translation="MKYKIIKTKSQYKTYCKMLEELVYKTPKSKLIKEEIALLTLLIE
                     KWDEEHSPFTDKNPIVLIQALMEANNLKSKDLVKILGVSKGLISDILSYKKGLSKANI
                     RILASYFKISQEALNKPYKLKLQEEYA"
     gene            complement(4907..5239)
                     /locus_tag="PLB46_07615"
     CDS             complement(4907..5239)
                     /locus_tag="PLB46_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HRF76422.1"
                     /translation="MKVHLIKAQTIRDYVGKHKSGESSFENWITIIKVADWSNANDIC
                     ATFATADLLGNGSERVVFNIAGNKYRMICSYQFGEKEVHLFVCWIGTHAEYDKLCKQG
                     KQYTISEY"
     gene            complement(5501..>6637)
                     /locus_tag="PLB46_07620"
     CDS             complement(5501..>6637)
                     /locus_tag="PLB46_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76423.1"
                     /translation="KKAEKEKESTESELMHLIRTSRATKNFQVSFALNVMQGTPESNL
                     RAITTLIDSGVNIVAVVAGNETFASYNYNWEKYKNDFEPILKAIQKKYPDIPRLLCIG
                     QAINRKTHIQWNTQLFQYVKNTGNFISGVDIHYYLYDELKEANATHPGMVQIKEGKAD
                     KQLDKAFAMYIDAYRNKDQMSALAAYLQVNLPGKIYHCSEFGDKEAENWSNTIANGAH
                     IFTTFCVNRNNFDMLLVHNLIGNWLWAARRPATKLDDVKNKSEKVNRISWFALQLANE
                     LPVTALPLDKTIKITSPGNYYYYYDNAGGELFNPEITSTFSTITYELHYVTGKFPYSS
                     AGSTGFWAKNSEIFFEVKGITIDKQKNQLSIPKNSFGYVQVVVE"
BASE COUNT         1916 a         1153 c         1220 g         2148 t
ORIGIN      
        1 gtaaaaaaca caacagtttg ttgtcagttc acaagcaacc tgttaagggt tgttaacgtc
       61 tgacaatcga ttggcatttg aagcatcatg gtaagaagat attcccaatt tctcgaactt
      121 gtttacttcc tggtagacat tttgctgctg aacatttcgt tcctgctggc catgctgatt
      181 agtttcgaca ggatttcaaa agttactagc gaccgtaaat ttgccatgtt attagtggcc
      241 gtaaacctga tttggttttt ggtgaccagt gttttcaact actataaaac cgaacgcgag
      301 cgccgcacag gatacgaaca tctggtattc cgttttatca aagtattggc atttcaggcc
      361 ctgctcacat tcacgtattt gtttttaatc aaagggtatc agttttcacg ttcacaacta
      421 gcttatacct atattttctt taccgtaatt gatttggcat ggagaatagg gtttgaaacc
      481 tatctcaaac gttacagagc aagaggtggt aattatcgcc gtattgttgt ggttggtact
      541 accaatgcaa gtttgcagtt tgtgaaagaa attcaggaac acaacgaatt tggttatcgg
      601 tttatgggtt atttcgacga cccggaaact tctatggcag aggttacagg ttcattacat
      661 caggtaaaag ccttttgtat tgaaaataat atcgatgaaa tttatttcgc ccttcagcct
      721 tccgatatga ttaattatgt ggacgacctg atggaattta gtaatgataa attaatacga
      781 ttcagaattg gtccagagtt cagcaactag ataagcaaac aatttaaaaa agtaaatagc
      841 gaatactacg gcaatacccc cattttgagt attcgccctg aacctttaga taatatttat
      901 aaccgcctgc aaaaacgttt atttgatatc atttttgcgt tgctgttttt tattataatt
      961 ggttggtggt tattcccaat aatcgcatta ttcgtaaagt tatcctcacc cggaccggta
     1021 ttttttgtgc agaagagaag tggggaaggc aacgaagagt tttggtgtta caagtttcgt
     1081 tcaatgaaag taaatgcgga agccgatacc aaacaggcta cagttggcga tgcgcgtatt
     1141 accagagtag ggtcattcct gcgtaaatca aatctcgatg agttaccaca atttatcaat
     1201 gtgctgttgg gcgatatgag tgttgttggt cctcgtccac atatgttgaa acatacagaa
     1261 gaatattcaa aaatcgtgaa taactttatg gtgcgccatt ttgtgaaacc gggtattaca
     1321 ggtgcggctc aggcaaatgg atacagaggt gatactaccg accccgttat gatggaaaaa
     1381 cgggtgcagt acgacctttg gtatcttgaa aactggagct tctggctgga tataaaaatc
     1441 gtgtttctca cagtctggag tatgattaaa ggaaatgaaa atgcggttta aactactaat
     1501 gcttctctgc tgcggcgcac tcgctgtttc ggcacaacaa aattatttac ctcttaacag
     1561 aaacatttac aactggtacg aaccggcttt aaataagccc ggaaccggtt tccatacggc
     1621 aattcggcct tatcgtatca gcgatatgga tacagttgtt acgtacgacg atgtttttca
     1681 tcttaaaaaa gtaagtggtg atggcttttt ttccaaactc gggaactgga ctgcttacga
     1741 taccattttg ggtgtgcaca aaacagaatt ccatttgcgt atagacccaa ttgtgaattt
     1801 tggtggtgga ttaaatttag aaaacggggg acaaggtgtt tggaataata ccagaggtat
     1861 aggtttttcc ggtgatatcg gcgaaaaaag aaatgtaagt tttaatatca ccctgcgcga
     1921 aaatcaaggt aaatatcccg attatgttac ggcttttgta aaagaatata atgttgttcc
     1981 tggccaggga aaggtgcgaa attttaaagg aaatgcgtac gattttgcca attcaagtgg
     2041 ttatgtttct tacaatgctt ccaaacattt taattttcaa ttaggtcacg ataaaaactt
     2101 tttgggagat ggctatcgca gcatgctgtt aagtgataac gcgatgtttt atccgttttt
     2161 taaaatcacc acatctgtct ggaaaattaa atacatgaat ttatatactg aatttgacga
     2221 tgcctataat ggcacaatcg attcggttgt tggtgtaacg cgtaaattcg gttcgtttca
     2281 ttatttaagt tgggatgttt ttccttgggt gcaattaggt ttgttcgaag gaattgtatg
     2341 gccggcaaat gattcaacta gatatagagg gtttgatgta aattatatta atccgatcat
     2401 atttttccga ccgatagaat ttggtttagg ttcacccgat aatgccgtaa ttggagcaaa
     2461 tattaaattc acaccattag aaaaactgat tatttatggt cagtttatta tggacgactt
     2521 agacgtagct gctgcgaggg caggggatgg gtattataga aataaatttg gtattcaggg
     2581 aggatttaaa ttatacgagc cgtttaaaat taaaaacctg attgtgcagg cagaaatgaa
     2641 tcaggctaat ccgtatactt atgcaagtaa agagccggtg caaaattatg cgcattataa
     2701 tcagccctta gcacatccgc ttggtgctaa tttcagagaa tttattttta ttggtaatta
     2761 tcgttttttc gaaagatttt atctggaagc aaaattacag tccgccgtaa aaggagtaga
     2821 tgccaataat gccgagcaca atggtggaga tattttcacc tccgatttcg aaattccggg
     2881 cttccccgat tcgtatggta attttatcgg acaaggatta aaaacaacaa ttaaaagtac
     2941 agacctgcgt gcgggatatt tattaaatcc ttctattaat ttaaatattg agggacagat
     3001 atttatcaga aattataaaa atgataaaga tgtactgtcc actactatgg taatgttatc
     3061 gctcaaaacc gatttgttta atgcctatta cgatttttaa ggcctgaaat attgttttat
     3121 ttttgccgtt agttctttat gtgatgtata gaggggatat gctatgatgt gtggcgtacg
     3181 aaatgaataa attagtcctc cgcctcggcg gacgtactta ataaataact tggtcccgta
     3241 gttcaacgga tagaatagga gtttcctaaa ctctagatac aggttnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnggtcc cgtagttcaa cggatagaat aggagtttcc
     3421 taaactctag atacaggttc gattcctgtc gggaccacta ttaaaatgta aaaaggtgtt
     3481 tcctaaactc tagatagcag ttccctgccc gacttgcatc agcaggcggg gattcctgtc
     3541 gggaccacaa aagttaacgt ttttgagttc ctccatgtcg caaaaaccgt cccgctgttg
     3601 cgggacactt tctaatgatt aattttgtgt tgatttttgg ctccgtagta taatggatag
     3661 aatgcctgcc ccgctgacta aagtcagagg gggaagattc cggttcttca gatgnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaattt aaaagtatct taatacctat
     3841 cttgggtata gaacagtgat tattctccca ccaattatta attattaatt ttacatttta
     3901 aactggctcc gtagtataat ggattgaatg cctgccccgc tgactaaagt cagaggggga
     3961 agattccggt tcttcagatg gcggtttcac caatttaaaa gtatcttaat acctatcttg
     4021 ggtatagaac agtgattatt ctccctccaa ttattaatta ttaattttac attttaaact
     4081 ggctccgtag tataatggat agaatggaag attccggttc ttcagatggc ggttcgattc
     4141 cgcccggggc taccaccatt tataaaactg gccaatcagc atttcgcttt ttggtctttt
     4201 ttttaccctt aaatcattac ttatgacagt ttttgtttat gcccttaaaa accaaatcaa
     4261 tgatgaaatt tatgtaggaa tttctgctga ccttgatcga agattaaaag aacacaattc
     4321 aggaaaaaac aggtatacaa aagcatttat accttggtct atattttttt ctgaatcctg
     4381 cgaatcttat gctgaagccc ggaaacgtga aatctattat aaatcagcgg cgggtaaacg
     4441 gaaattaaaa aataggtaga aaattattct tagttgttgt taacctatat acttgaggtt
     4501 taggcatatt cctcttgcag ttttaattta tatggtttgt ttaatgcttc ctgcgatatt
     4561 ttaaaatagg aagccaaaat acgtatattg gctttggaca accccttttt ataacttaaa
     4621 atatcagaaa ttaatccttt actaacgcca agaattttaa ccaaatcttt tgattttaaa
     4681 ttgtttgctt ccataagggc ctgtatgagc actatagggt ttttatctgt aaaaggggaa
     4741 tgttcttcat cccatttctc aattaataaa gtaagtaacg ctatttcctc ttttattaat
     4801 ttagattttg gtgttttata aaccaattct tcaagcattt tacaatatgt tttgtattgg
     4861 cttttagttt tgatgatttt atatttcatg actaatacat ttttcattaa tattcactga
     4921 ttgtatattg tttcccttgt ttgcaaagtt tatcatactc cgcatgtgtg ccaatccaac
     4981 aaacaaataa atgaacctct ttctcaccaa actgataact gcaaatcata cgatatttat
     5041 tgcctgctat attaaaaacc acccgctcac ttccattacc taataaatct gcagtggcaa
     5101 acgttgcaca aatatcattg gcattagacc aatcagcaac cttgattatg gtaatccaat
     5161 tttcaaaaga actttcaccg gacttgtgtt taccaacata gtctcttata gtttgcgctt
     5221 tgataaggtg gactttcata ggtttaacgg taaaggtaaa aaaaagttca caatatgtga
     5281 acttttatgc aattttttaa ggcaacttcg ccacctttta ctattatgtt gctcggtcaa
     5341 tcccggtagt tttgtaggtc agagctgaaa ctatgggcaa gaaatacctt ggtccaacgc
     5401 tggattatca gactttaggt caatttagca aagtaaacct gctgtacaga taaaatgtaa
     5461 tgaaatcctt tttgtaagtt accattccgg catttctcaa tcactccacc accacctgca
     5521 catacccaaa actattttta ggaatgctga gttgattttt ttgtttatcg atggttattc
     5581 ctttcacctc aaaaaatatt tccgaatttt tagcccaaaa accggttgaa cctgcactgc
     5641 tatatggaaa ttttcccgtt acataatgca attcataagt tattgttgaa aatgtagaag
     5701 taatttcagg attaaataat tcaccacctg cattgtcgta atagtaataa taattacccg
     5761 gtgatgttat tttaatagtt ttatctaatg gcaaagcagt cacaggtaat tcatttgcca
     5821 attgtaaggc aaaccaggaa atacgattta ccttctccga tttatttttt acatcatcca
     5881 atttggtcgc aggcctgcgc gctgcccata accaattgcc aatgagattg tgtaccaata
     5941 acatatcaaa attgttgcgg ttgacacaaa atgtggtaaa aatatgtgca ccattggcga
     6001 tagtattcga ccagttttct gcttccttat cgccaaactc tgaacagtga taaattttac
     6061 cgggcaaatt cacttgcaaa taagctgcta atgcactcat ttgatctttg ttgcgataag
     6121 catctatata catggcaaat gctttatcta attgtttgtc cgctttgcct tcttttattt
     6181 gaaccatacc aggatgtgta gcattggctt cttttaattc gtcgtacaaa taataatgaa
     6241 tatcaacccc tgaaataaaa ttgccggtat tttttacata ctgaaataat tgggtattcc
     6301 actgtatatg tgtttttcta tttattgcct gaccaataca taataacctc gggatgtcag
     6361 gatatttttt ttgtatcgct tttaaaatag gttcaaaatc gtttttatat ttttcccaat
     6421 tgtaattgta actggcaaat gtttcattac cggccacaac cgcaacgata tttacgcctg
     6481 aatcaattaa tgtagtaatt gcacgcaaat tcgattccgg cgttccctgc atcacattta
     6541 atgcaaaact tacctgaaaa ttttttgtcg ccctgctcgt acgtataaga tgcatgagct
     6601 cactttcggt agactccttt tccttttctg ccttttt
//
LOCUS       DAORKW010000156         6621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_398_length_6621_cov_4.486750, whole genome shotgun sequence.
ACCESSION   DAORKW010000156 DAORKW010000000
VERSION     DAORKW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_398_length_6621_cov_4.486750"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="PLB46_07625"
     CDS             complement(<1..1895)
                     /locus_tag="PLB46_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76424.1"
                     /translation="MKPHNILFNTFGAILLQFAFSSLQAQPFSWQNTLGGSQNEFVKS
                     TVGTSDGGYIFAGYSSSPMSPDKSENIIGGYDMWIVKTDSVGNIEWDNTIGGLADDAA
                     NSIVQTFDGGYVVAGYSGSSISGDKTEASKGGNDFWIVKLSATGAIEWQNTIGGNDND
                     QATAIKQTFDLGYIVVGFSLSGISGDKSEANKGPSFTSDYWVMKLDNSGNLLWENTIG
                     GDNYDYIYTVETTWDGGYIIGGYSQSGISGDKTEVLIGNADYWVIKLNHLGNIQWQNT
                     IGGTGAEIVRKIILDTDGGYVVGGYSSSDIDGDKTEIAFGLSTDYWIVKLNSSGNIVW
                     QNNIGGNDTEILYDMIKLDDGGFGLFGLSYSGISGDKTEANIGGSDYWYVELSSTGDL
                     VQQNTIGSTTEDQSASIAQGENLEIVLAGSSKSPAGIDKAENNLGGPETYDFWIVTMY
                     AECIPFEEICNTVDDNCNGLIDEDITDSITITATGSTTICQGETVTLNATYTGTTVQW
                     KRNGAPIAGATSPTYITSQKGFYTCYTESECASAESGGINVVVNKKPSAAITPAGPTS
                     FCAGGSVTLNVTPVGGCSYQWYKGASLIAGATGLSYVATTPGNYKCQVTKIATGCFKN
                     SNSIAVFV"
     gene            complement(1915..4116)
                     /locus_tag="PLB46_07630"
     CDS             complement(1915..4116)
                     /locus_tag="PLB46_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76425.1"
                     /translation="MFINACSNYSTNKTNKLSLVIICFVMSFCFVTTLSAQFPEMVWQ
                     GTYGGSSYDFTVQTAVTSDGGYIMAGTAQSGISGNKTIAAYGAEDYWIIKLDAIGNIQ
                     WQKVFGGTGSDVAAHAEQTADGGYIIGGYSSSLPSGNKTAPLHGIYDCWILKLNASGI
                     IEWQTSLGGDQNENCYAVHQTPDGGYIVGADSESDISGNKTTPSFGGKDYWVIKLDAS
                     GTKVWEQAYGGNATDLFADINLTADGGFIIGGSSNSNFSGNKNENTNGLNDFWVLKLS
                     NTGTIEWQNTIGGSHNDDVYFCQQTLDGNYFVGGSSASEISGDKTEAQFGAAVGLGDY
                     YVMKLNTSGDIIWQNTIGATREDQCYAGMQLSNGNYIIGGMSGSTANGDKTLPRIGGA
                     DIADIWVLILDTIGNISSQNVIGGTNSDIAFGFCEGADGGILISAVSWSEINGNKTSR
                     WYGQDDAWVIKLSGACVPTTEICNTLDDDCNGLIDDGITETISIEAAGATTVCQGETV
                     LLNATYSGTSIQWKKNGVSMAGATADSYTATATGIYTCETSSACASNISNEISVTVNK
                     NPKASITAGGPTTFCAGGSVTLTETPVGGATYQWFKGGAAIGGATTNVYVATTSGNYK
                     CRVTKTATGCIKMSNAIAVLVTCKEGENIAANNWTIYPNPAHDNLMLQTIDMSQSNKE
                     FMIVDVTGKTVQTITATANETVIDLSTLPNGLYLIQSVGDSTAISKAFVKQ"
     gene            complement(4131..6104)
                     /locus_tag="PLB46_07635"
     CDS             complement(4131..6104)
                     /locus_tag="PLB46_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76426.1"
                     /translation="MSGFMDYWVMKLDASGNIVWQNTIGGSKDDFCFDGHATADGGYI
                     LAGQSKSNISGDKTENTLGFFENTDMWIVKLNSSGAIEWQNTIGGLHIDFAEIIRELP
                     DGSFIVGGNSSSGIGYDKTVDLHGTGGLTYDIWVLKLSSTGSIIWQKNIGGASDDYFS
                     DLILTADGGFLIGAESYSGIGFEKTAPLIGVFDNWIIKLDADGNIEWDKTIGGDNLDN
                     IGGLAQTTDGNYVVGAHSMSGISGNKTEINNGTDYWVYKLDTLGNIIWQNTIGGLSTD
                     FLYAIAATDDNGVIVVGESNSGANIDKDEVSIGGSATDYWIIKINQTGGICWQETIGG
                     NNQDVGRTIYQKSPGIFVVGGYSYSNATGDKNENNASGTTYPDYWLMEIDDDFIPGLE
                     LCNGYDDDCDGMVDEDINETISISAGGATTFCQGSNVVLSATYSGTSLQWKKNGLIIP
                     GATTASYTANATGNYACATYSDCDTTTSSEIVVTVNKNPKASIYAGGPTSFCPGGSVT
                     LNVMPVGGATYQWYKGASLIGGATSTTYVATTSGNYKCRVTKTATGCFKNSNVIAVIV
                     SCKEGEGSIDIENNYLEIFPNPASEMITITLNAATDHLELFEVLDATGKMVLTFNLEN
                     NQSVLNISKLASGLYYIKSSSQLNALQTSFIKQ"
     gene            complement(6055..6321)
                     /locus_tag="PLB46_07640"
     CDS             complement(6055..6321)
                     /locus_tag="PLB46_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76427.1"
                     /translation="MKLPHTLKGIFIFFLITYFPLFASAQTINWQNTIGGTNDDKLWR
                     TGPTLDGGTFCVGFSKSGIGGDKTEAAIGVGFYGLLGDEIRCLR"
     gene            complement(6324..>6621)
                     /locus_tag="PLB46_07645"
     CDS             complement(6324..>6621)
                     /locus_tag="PLB46_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76428.1"
                     /translation="NSNSIAVFVPCKEGEELTEHADLLVYPNPANQFINVKLNNATTE
                     QTEIVICDATGKNVLDQQCLGYEISIDIAGLIPGVYFIQSVSDHQIHNSSFIKQ"
BASE COUNT         2004 a         1458 c         1015 g         2144 t
ORIGIN      
        1 acaaaaacag caatactatt cgaattttta aagcaaccgg tagcaatttt agttacctga
       61 catttataat taccaggtgt tgtagctaca taactcaaac cggtggctcc tgcaattaaa
      121 ctggcacctt tataccactg gtaactgcaa ccaccaacag gcgtaacatt cagcgtaacg
      181 ctacctcctg cacaaaatga ggttggtcct gcaggtgtga tagcagcaga aggtttttta
      241 ttaactacta cattaattcc gcctgactca gctgacgcgc attcgctttc agtatagcag
      301 gtataaaatc ctttttgtga agtaatatat gttggcgaag tagctcctgc aattggagcg
      361 ccattgcgtt tccattgcac tgttgtgcct gtataagtgg cattaagcgt tactgtttca
      421 ccttgacaaa ttgtggtaga acctgttgca gttattgtta tagaatcagt tatatcttca
      481 tcaattaaac cgttacaatt atcatctacc gtattacaaa tttcttcgaa cggaatacat
      541 tccgcataca tagtaacaat ccaaaaatca tacgtttcgg ggccgcctaa gttattttca
      601 gctttatcaa taccagcagg cgattttgaa gaacctgcta atacaatttc aagattttca
      661 ccttgagcaa tacttgcaga ttgatcttct gttgtacttc caattgtatt ttgctgcact
      721 aaatcacctg tggacgataa ttcaacatac caataatcgc tgccaccaat gtttgcttct
      781 gttttatcac ctgaaatacc tgaataacta agcccaaata atccgaatcc gccatcatcc
      841 aattttatca tatcgtataa tatttcggtg tcgttgccac caatattatt ttgccaaaca
      901 atattgccgc tcgaatttaa tttcacaatc caataatcag tgcttagtcc aaaagcaatt
      961 tctgttttat cgccatcaat atctgatgac gaatatcccc ctactacata accaccatct
     1021 gtgtcgagaa ttattttcct gacaatttca gcacctgttc ctccaatagt attttgccat
     1081 tgaatattgc cgagatggtt taattttatt acccaataat cggcgtttcc tattaatact
     1141 tctgttttgt cgcctgaaat tccggattgc gaataaccgc caatgatata accgccatcc
     1201 caggttgttt caacagtata gatataatcg taattatcgc cacctatagt gttttcccat
     1261 aataaattac cggaattatc gagtttcatc acccaataat ctgaagtgaa tgaaggtcct
     1321 ttgttagctt ccgatttatc gccacttata cctgataatg aaaaaccaac tacaatatat
     1381 cctaaatcga aggtttgttt gatcgctgtt gcctggtcgt tatcattccc gccaattgta
     1441 ttttgccatt cgatggcacc agtagcactt aattttacta tccaaaagtc atttccacct
     1501 tttgatgctt ctgttttatc tcccgaaata ctggaaccag aataaccagc tacaacataa
     1561 ccaccatcaa atgtttgcac aatcgaattg gcagcatcat ctgctaatcc accaattgtg
     1621 ttatcccatt caatattgcc aacggaatcg gtttttacga tccacatatc ataaccacca
     1681 ataatgtttt ctgatttgtc cggtgacata ggcgaacttg aatatccggc aaaaatatat
     1741 ccaccatcag acgtacctac ggtcgatttt acgaattcat tttggctgcc gcctaatgtg
     1801 ttttgccaac taaaaggttg cgcctgaagg ctactaaacg cgaattggag taaaatagca
     1861 ccaaatgtgt tgaataatat attgtgtggc ttcattatga aaccaaatat actattattg
     1921 tttgacaaac gccttcgaga ttgctgtgct atcacctacc gattgaatca aatacaatcc
     1981 atttggaagg gttgataaat ctatcactgt ttcattcgct gttgcagtta ttgtttgcac
     2041 cgttttacca gttacatcaa caatcataaa ttccttattg gattggctca tgtcaatggt
     2101 ttgtaacatc aaattatcat gtgcaggatt tgggtaaatt gtccaattgt tggcagcaat
     2161 attttccccc tctttacaag taaccaatac cgcaattgca ttcgacattt taatacaacc
     2221 tgttgctgtt tttgttacac gacatttata attgccggaa gttgttgcta catatacatt
     2281 tgtagttgca ccaccaatgg ctgctcctcc tttaaaccat tggtaagtag caccgcctac
     2341 aggtgtttca gttaatgtaa cacttcctcc agcacaaaaa gtagttggtc caccggctgt
     2401 aatacttgct tttggatttt tatttacggt aacactaatt tcattagaaa tatttgatgc
     2461 acatgcactg cttgtctcac aagtataaat ccccgttgct gttgcagtat aagagtctgc
     2521 cgttgcaccc gccattgata caccattttt tttccattga attgaagttc cggaatatgt
     2581 tgcattaagc aatacagttt caccctgaca aacagtggta gcaccggcag cttcaattga
     2641 tattgtttcg gtaatgccat cgtcaattaa cccattacaa tcatcatcca acgtattaca
     2701 aatttcagtt gttggcacgc atgctccact tagttttatt acccatgcat catcctgacc
     2761 ataccaccga gatgttttat tgccgtttat ttcagaccaa ctcactgccg aaataagaat
     2821 tcccccatct gcaccttcac aaaaaccgaa agcgatatca gaatttgttc cacctattac
     2881 attttgagaa ctgatatttc cgattgtatc aagtatcaat acccatatat ctgcaatatc
     2941 ggcaccacca atacgtggaa gtgttttatc accatttgca gtggaacccg acataccgcc
     3001 aatgatatag ttgccattgc ttaattgcat gcctgcgtaa cactgatctt cacgcgttgc
     3061 gccaatggta ttttgccaaa taatatcacc tgatgtgttt aatttcatta cataatagtc
     3121 acctaatcct acggcagcac caaattgcgc ttctgtttta tcaccactaa tttcagatgc
     3181 cgaggagcca cctacaaaat aattaccatc taatgtctgc tgacaaaaat acacatcatc
     3241 attatggctg ccgccgatgg tattttgcca ctcaattgta ccagtgttgc ttaatttcaa
     3301 aacccaaaaa tcattgagcc cattggtgtt ctcattttta tttccggaaa aattagaatt
     3361 agaactacca ccaataataa atccgccatc ggctgttaaa ttaatatcag caaataaatc
     3421 ggtagcattt ccaccgtaag cttgttccca cacttttgtt cctgaagcat ctaatttaat
     3481 tacccaataa tctttacctc caaatgaagg tgttgtttta tttcctgaaa tatcagattc
     3541 actatcagct ccaacaatat atccaccatc aggagtttga tgcaccgcat aacaattttc
     3601 attttgatcg ccacctaagg aagtttgcca ttcgataata cctgaagcat ttaattttaa
     3661 aatccaacag tcataaatac catgaagtgg tgctgtttta tttccacttg gcaaagacga
     3721 cgaataaccg ccaataatat agccgccatc agcagtttgt tcggcgtgtg cggcaacatc
     3781 tgagcctgtg ccaccaaata ctttttgcca ttgaatatta ccaattgcat ccaatttaat
     3841 aatccaataa tcttcagctc cataagctgc aatggtctta ttgccggaaa ttccggattg
     3901 cgctgtacct gccataatat agccaccatc ggaagttaca gcagtttgta cagtgaaatc
     3961 ataagaagaa ccaccataag tgccctgcca caccatttca ggaaattgcg ctgataacgt
     4021 agttacaaaa cagaacgaca tcacaaaaca aataattaca agtgataatt tatttgtttt
     4081 gttggtggaa taatttgaac aagcgtttat gaacatataa tggaaaatta ttactgttta
     4141 atgaatgaag tttgtaaagc atttaattga gatgaagatt taatataata taatccggaa
     4201 gccagttttg aaatatttaa tacagattga ttattttcca aattaaatgt taaaaccatt
     4261 ttgccggttg catccaatac ctcaaacaat tcaagatggt cagttgctgc atttaaggta
     4321 attgttatca tttcgcttgc cggattaggg aatatttcca ggtagttatt ttctatatca
     4381 attgaaccct ctccttcttt acaagaaaca attaccgcaa ttacatttga atttttaaaa
     4441 caacctgtgg cagtttttgt aacacgacat ttataatttc ctgaagttgt tgcaacataa
     4501 gttgtagatg tagcaccacc aattagtgat gcacctttat accattgata agtagcgcct
     4561 ccaaccggca ttacattgag tgtaacactt ccacccggac aaaatgatgt tggtcctccg
     4621 gcataaatac ttgctttagg atttttattt acagtaacaa ctatttctga agaagttgtt
     4681 gtatcacaat cgctatatgt tgcacaagca tagtttccgg ttgcattagc cgtataactt
     4741 gctgttgttg caccgggaat tattaaacca tttttcttcc attgtaatga agtacctgaa
     4801 taagttgcag ataacactac attactaccc tgacaaaatg ttgtagcacc acctgctgat
     4861 atcgaaatgg tttcattgat atcttcatca accattccgt cgcaatcatc atcataacca
     4921 ttacataatt ccaaacccgg aataaaatca tcatcaattt ccattaacca gtaatccgga
     4981 taggttgtac cgcttgcatt attttcgttt ttatcacctg ttgcattcga ataggaatat
     5041 cctcctacaa caaatatccc cggtgatttt tgataaattg ttctcccgac atcctgatta
     5101 tttccaccta ttgtttcctg ccaacaaata ccaccggttt gatttatttt aattatccaa
     5161 taatccgttg cgctgcctcc aatggacact tcatctttat caatatttgc acctgaatta
     5221 gattctccaa ctacaattac accattatca tctgttgcag caatagcata taaaaaatca
     5281 gtgctcaatc caccaattgt attttgccaa atgatatttc ccaatgtatc caatttgtaa
     5341 acccaataat ctgttccatt atttatttct gttttatttc ctgaaatccc tgacatcgaa
     5401 tgtgcaccta ccacataatt gccatcagta gtttgtgcta agccgccaat attatccaaa
     5461 ttatctccac caatagtttt atcccattca atattgccat cagcatccag tttaattatc
     5521 caattgtcga acacaccaat tagtggtgct gttttttcaa atccaattcc ggaatatgat
     5581 tctgctccta ttaaaaatcc gccgtcagct gttaatataa gatcagaaaa ataatcatca
     5641 gaagcacctc caatattttt ttgccagatt atgcttccgg tggatgataa tttcagcacc
     5701 caaatatcat aagttaatcc gcctgtacca tgtaaatcaa ctgttttatc atagccaatt
     5761 ccggatgaag aatttccgcc aactataaaa ctaccatccg gtaattcacg aattatttca
     5821 gcaaaatcga tatgtaatcc gccaattgta ttttgccatt ctattgctcc ggaactattt
     5881 agttttacaa tccacatgtc cgtattttcg aaaaagccaa gtgtattttc agttttatcg
     5941 cctgaaatat ttgacttcga ttgtccggcc aaaatataac caccatctgc ggtagcatgt
     6001 ccgtcgaaac aaaaatcatc tttacttcct ccaatagtat tttgccatac aatgttaccg
     6061 gaggcatcta atttcatcac ccaataatcc ataaaacccg acaccaatag ccgcttctgt
     6121 tttatcgcca ccaattccgg attttgaaaa tccgacacaa aaagtgccac catctaaagt
     6181 tggtccggta cgccagagtt tatcatcgtt ggtgccacct atggtgtttt gccaattaat
     6241 agtttgagct gacgcgaata aagggaaata ggtaattaaa aaaaagataa atataccttt
     6301 aagtgtgtgc ggtaatttca tgcttattgt ttaataaatg aactgttatg gatttgatgg
     6361 tccgaaacag attgaataaa ataaacacca ggaattaatc ctgcaatatc aattgatatt
     6421 tcataaccca aacattgttg atccaacaca tttttacctg tagcatcaca aattactatt
     6481 tcggtttgtt cagtagttgc gttatttaat tttacattaa taaattgatt agccgggttc
     6541 ggatatacca gcaagtcagc atgctcagtt agctcctctc cttctttaca aggtacaaaa
     6601 acagcaatac tatttgaatt t
//
LOCUS       DAORKW010000157         6606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_401_length_6606_cov_5.081514, whole genome shotgun sequence.
ACCESSION   DAORKW010000157 DAORKW010000000
VERSION     DAORKW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_401_length_6606_cov_5.081514"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLB46_07650"
     CDS             complement(<1..212)
                     /locus_tag="PLB46_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76429.1"
                     /translation="MKSILTAIGVLLCVVTARAQFIENFDDGDFAGWTGIDTGYIVNA
                     SNQLQLHGNCSTGGIEYLSYPLATLDS"
     gene            complement(384..1643)
                     /locus_tag="PLB46_07655"
     CDS             complement(384..1643)
                     /locus_tag="PLB46_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF76430.1"
                     /translation="MGRSVSGAGDVNNDGFPDVMASANFYDNGQLNEGVVYVYHMCAD
                     SLYADLDGDGFGDPFNLVNICNDTINLVEDNTDCDDTNASIYPGAIEICNSLDDDCNT
                     LIDEGLIFEIYYVDADADFFGDINDAGTSACLPIAGTVQNNTDCDDANGDINSAETEI
                     CNLIDDNCDGFIDEGFDVFITTTALTGTTFCQGGSVVLEASHNGIALQWKKNGAIIPG
                     ATSTTYTATKTGTYTCEASNLCNTALSTGIVVTANKNPNANITAGGPTTFCPGDNVNL
                     NVTPVGGSAYQWYKGPTPIAGATSLTYTATTSGNYKCRVTKIATGCYKNSNTIAVNVM
                     CKELVVSTQIDFTIFPNPATQDITITTQDVSAKSIYIIDAVGQVVQTILTEQTMINID
                     IQPYANGVYFIKINSETGSITKSFVKE"
     gene            complement(1656..2870)
                     /locus_tag="PLB46_07660"
     CDS             complement(1656..2870)
                     /locus_tag="PLB46_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HRF76431.1"
                     /translation="MKLLAIAFTLFFFVITETNAQTPPPTTLLESNQIDGNYGWYISN
                     AGDINADGFDDVVVGADWYDNGQSNEGAVFVHMGSATGLETTPAAILESNQVEANMGR
                     SVSRAGDVNNDGYDDIIVSAWLYDPGVTNAGRVYIYHGSASGINTTPAKTIDGNQFGA
                     NFGNCVSAAGDVNNDGYDDVVVGAWLYDAGQTDEGRVYVFLGSSTGITTPAVVVLEGN
                     QEFAHFGNSAAGAGDVNADGYDDIVVAADRFDGGLSNEGKVLVYHGTATGINPIPAKE
                     MEGNLDNANFGSEVASAGDVNNDGFDDIIVGAHKYEDGMADEGAAFIFHGSATGIITT
                     PVTMLQNNDVLAYFGIAVRGAGDFNQDGYDDVMVGSILFENGSAAEGSAYLYKGSASG
                     IKTTPYLTIEGN"
     gene            complement(3431..4576)
                     /locus_tag="PLB46_07665"
     CDS             complement(3431..4576)
                     /locus_tag="PLB46_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76432.1"
                     /translation="MFLKQNNNIEIITITVNPIYTSVILPIIGLFIGLLVLFGINKYS
                     SFYVNKGEVILLAAVPKTEVKDTLTQQLYTYDTTSVWVYNDSNYVKLKTPKQIKGLRA
                     TYSSDVEFFRDYPSFMLWCLFILIQFAVWMFIICALPMGLHHLKVENKFKLFKTGLFV
                     SLLFFIVFLVLQTRTFYDSEPIRSDVFTSNLDNKILFATIIGSIAGAVCFAVMFTAVG
                     ILKENTAKDQTIEAQAIKLFNYSLSAASLILSLMVFSMSSLHTAYIDLLSRYYVKINL
                     NYDIVYAYGLLYTLILLIFYVPLKLVSNNYIYFTDVNQQQTPAVNVVPDLQPNLVAPQ
                     PVPQNVVGIVKGPNNLKAFGDFIKGLTVSLSPFIASVIGNLLDKVFG"
     gene            complement(4557..5792)
                     /locus_tag="PLB46_07670"
     CDS             complement(4557..5792)
                     /locus_tag="PLB46_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76433.1"
                     /translation="MKNYLIFFLLVGLVHSNSKGQVGDPLLKDTLLKATQYSIPSSGA
                     FTLLGVNPENVSTPGFSRDFKIDYFFQDAQLNPNIALQCQPFWLLFYKNKTIDSWSDA
                     NFLHSIVANTEFSFGSTENQTKGNQFAYSFKTTIGYNPLTDTSFSKGYETVLGAVASS
                     INQLNMNLTMEKIAIKFKQDSLENEAFNLKTELSNLSNYQTLEKNKLISEINTINAQI
                     AEMTSRVFIINSTIEDNLNNENKKLEENAKKYVSNYMETKKSKFRMDIGFGQVFQYKD
                     TLVTELNPLLTGFGIWINPGFGFCLDKNKSSCNNSLLFAAIGKLTVIDTITEITSGFN
                     IRYVGGKTDVFTEYSPVFTGQQLTANRISFGGGYELDNQKYLQIGFLMDFDQKFKLTK
                     IQPTFMVNLELGRDVFKTK"
     gene            complement(5802..6170)
                     /locus_tag="PLB46_07675"
     CDS             complement(5802..6170)
                     /locus_tag="PLB46_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76434.1"
                     /translation="MHKENIVYFSPQGSVVTLILKPFYATNGAYDIYITNGTQMQKIG
                     KGSFSDGIEDVFILPIVPAQLSSWVVVIAGAFASAYGHSQILVHYTFYQNGVALSEQI
                     TIEHIGSSLATQNDVYFKKI"
     gene            6370..>6606
                     /locus_tag="PLB46_07680"
     CDS             6370..>6606
                     /locus_tag="PLB46_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76435.1"
                     /translation="MKNRITFILIALIIVALSVLGTYSWLKQTAQLKSAEDAERIAAM
                     KKLEDSYKTNFDCENTYNIIDFNALLNNLLAAPIT"
BASE COUNT         2134 a         1248 c         1085 g         2139 t
ORIGIN      
        1 gagtcgagcg tggccagggg ataactcagg tattctattc cacctgtact gcagtttcca
       61 tgcagctgaa gctgatttga agcgtttaca atgtagccgg tatcaattcc tgtccacccc
      121 gcaaaatcac catcatcaaa gttttcgata aattgtgctc tggccgtgac aacgcacagt
      181 aaaacaccaa tggctgttag tatgcttttc atgataattt gtggtggaaa aatacggatt
      241 ttttactgaa aattcggatt caccacattc agttggttga aatacataat gactaccttg
      301 attattaacg ccgttttatc aaattgttaa tttgcaggta atatactttt gatgatttag
      361 tatatagttt tttattttaa aaattattct ttcacaaatg atttcgtaat gctgccggtt
      421 tcgctgttta ttttaataaa atatacgcca ttggcatagg gttgaatatc aatattaatc
      481 atagtttgtt cagttaaaat tgtttgaact acctgcccga cagcatctat aatataaatt
      541 gattttgcag aaacatcttg tgtagtaata gtaatatctt gagttgcggg atttggaaaa
      601 atggtaaagt caatttgtgt tgaaacaact aattctttac acataacatt aactgctatc
      661 gtgtttgaat ttttataaca tcctgttgca attttggtta cacgacattt atagtttccc
      721 gacgtagtag cagtataagt taatgatgtt gcacctgcga ttggcgttgg gcctttatac
      781 cattgatagg cacttcctcc taccggtgta acatttaaat ttacgttatc accaggacaa
      841 aatgttgtag gtccacctgc agttatgttt gcgttagggt ttttattagc agttacaact
      901 atgcctgtcg ataaagcggt attacataag tttgatgctt cacaagtata agttcctgtt
      961 ttagtagctg tatatgtggt tgatgtagcc cccggaataa tggcaccatt ttttttccat
     1021 tgcagcgcaa taccattatg cgatgcctcc agcacaacac ttcctccctg acaaaaagtt
     1081 gtgcccgtta atgcggttgt tgtaataaat acatcaaaac cttcatcaat aaacccatcg
     1141 cagttatcat caatcagatt acatatttca gtttcagcac tattaatatc accattagca
     1201 tcatcacaat cggtattgtt ttgaacagta ccagctattg gtaaacacgc actagttccg
     1261 gcatcattta tatcgccaaa aaaatctgca tccgcatcaa cataatatat ttcaaatatt
     1321 aatccttcgt ctataagcgt attgcagtca tcgtctaaac tattacaaat ttcaattgct
     1381 ccgggataaa tagatgcatt tgtatcatca caatcggtat tatcttcaac taaattaatt
     1441 gtatcgttac aaatgtttac gagattgaat ggatcaccaa aaccatctcc gtctaaatca
     1501 gcatataatg agtcggcaca catgtgatat acatatacca caccttcatt taattgacca
     1561 ttatcataaa aattggcaga tgccataaca tcaggaaaac catcgttatt tacatcgcct
     1621 gcaccggaaa cggagcggcc catattgact tcagactaat tgccttcaat agttaaataa
     1681 ggtgttgttt tgattccgga agcggagcct ttatataaat aggcgctgcc ttctgccgca
     1741 gatccgtttt caaacaaaat gcttcctacc attacgtcat cataaccatc ctgattaaaa
     1801 tcgccggccc cgcgcaccgc tatcccgaaa taagctaata catcattgtt ttgcagcatt
     1861 gtcaccggag tcgtaataat gcccgtggcg gaaccatgaa aaatgaatgc agcaccttcg
     1921 tctgccatgc catcttcata tttgtgtgcg cctacgataa tatcatcaaa accatcgtta
     1981 tttacatcac cggctgaggc aacttctgaa ccaaaatttg cattatccag attgccctcc
     2041 atttcttttg ccgggattgg atttatcccg gttgcagtgc catgataaac caatacttta
     2101 ccctcgtttg ataatccgcc atcgaatcta tcggcagcaa ccacaatatc atcataacca
     2161 tctgcattta catcacctgc accggctgca gaattgccaa agtgtgcaaa ctcttgattc
     2221 ccttctaaaa caaccacagc aggtgttgta ataccggttg atgagccaag aaaaacatat
     2281 acccttcctt catcagtttg ccctgcatca tataaccacg cacctacaac cacatcgtcg
     2341 taaccatcat tatttacatc gccggcagcc gacacacaat taccgaaatt agctccaaat
     2401 tggttgccgt ctattgtttt tgccggcgtt gtatttattc cggatgccga accgtggtaa
     2461 atataaacgc gtccagcatt ggtaacaccg ggatcataaa gccaagcact tactatgata
     2521 tcatcgtatc catcattatt tacatctcct gctcttgaaa ccgagcgacc catattggct
     2581 tcaacctgat tactttctaa tatagccgcc ggtgtagttt ccaagccagt tgcagagccc
     2641 atgtggacaa acacagcacc ttcattcgat tggccattat cataccaatc agcgccaaca
     2701 acaacatcat caaaaccatc tgcattaata tcgcctgcat tagaaatata ccaaccataa
     2761 ttaccatcaa tttggttgct ttctaataag gttgtgggag gtggggtttg tgcattagtt
     2821 tctgttatta cgaagaaaaa taaggtaaac gcgattgcaa gcaacttcat atgtatcttt
     2881 ttagcgaatg aaatataaat atatgtgatt tggggttaat tcactgtttt ttcgtgattt
     2941 gtgcattgaa ataatcaata agtgataagg gaataataac cttaataaaa agcggggcct
     3001 actagccgta attgttataa gattaaaagg atgttgaaca aattggcaca aacatcagga
     3061 ctaattaaag caatttgagg ctgttttaat tccagccaat aatccaactc ataaattgca
     3121 aataggttga ctttaagtgc ccggttcgtg ctacatagtt atgcagaatt gtgtagaggc
     3181 actctaatag ctctcaacgc taaggcgtag cacatttaat tagaacgctt cttggctaat
     3241 aattaattat tcgaattagg gtgctatcgg tttagtaata aatatatttt taaaaagaaa
     3301 ctaaaaaaca aattagaaaa ttgaatatat taatcaataa atataagttg ctaaataacg
     3361 agtttatcaa aacaaaacgt ggccttcaac aagttctaat caataatcaa attgaaacac
     3421 tataaaacaa tcatccaaac actttgtcca ataaattacc aattacactc gcaataaaag
     3481 gagataaaga aactgttagc ccttttataa aatctccaaa tgcttttaaa ttattaggcc
     3541 ctttaacaat tcccaccaca ttttgaggaa ccggttgtgg tgctaccaaa ttaggttgca
     3601 aatccggtac aacattcacg gctggtgttt gctgttgatt tacatctgta aaataaatgt
     3661 agttatttga aaccaatttt agaggcacat aaaaaattaa taaaatcagt gtatataata
     3721 aaccataagc ataaacgata tcatagttca aatttatttt tacataataa cgggataata
     3781 aatcaatata agcggtatgt agtgatgaca tactaaacac cattaaactc aaaattaacg
     3841 atgctgccga taaactatag ttaaaaagtt taatcgcttg agcttcgata gtttgatctt
     3901 tggctgtatt ttcttttaaa ataccgactg cggtaaacat aaccgcaaaa caaaccgctc
     3961 cggctattga gccaattata gttgcaaata atattttgtt gtccaaattt gatgtaaaca
     4021 cgtcgctcct tattggctct gagtcataaa atgtacgcgt ttgtaaaacc agaaatacga
     4081 taaaaaacag aagcgaaaca aaaagccctg ttttaaataa cttgaatttg ttttctactt
     4141 ttaaatggtg taagcccatt ggtaatgcac aaataataaa catccaaaca gcaaattgta
     4201 ttaaaataaa taaacaccat aacataaatg atgggtagtc gcgaaaaaat tctacgtcac
     4261 tcgaataagt agcacgaagt cctttgattt gttttggtgt ttttaatttc acataattgc
     4321 tgtcattata tacccaaaca ctggtggtgt cataagtata taattgctgt gttaacgtat
     4381 cctttacttc tgttttaggc acggctgcta acagaattac ttctcccttg ttaacataaa
     4441 atgaagaata cttattaatg ccgaaaagca ccaaaagccc aataaataat cctataatgg
     4501 gtaggataac tgaagtgtaa atagggttca cggttatcgt tattatttca atattattat
     4561 tttgttttaa aaacatccct tccaagttca aggtttacca taaaagttgg ttgtattttg
     4621 gtgagcttaa atttttgatc aaagtccatc aggaacccaa tctgtaaata cttttgattg
     4681 tctaattcat aacctccgcc aaacgaaatt cgattcgcag ttaattgctg accggtaaaa
     4741 accggcgagt attcagtaaa aacatccgtt tttcccccta cataccgaat attaaatcct
     4801 gaagttattt cagtaatggt gtctataacg gtcaactttc ctattgcggc aaaaagtaaa
     4861 gaattgttgc aactggattt gtttttatct aaacaaaatc caaaccccgg attaatccaa
     4921 atgccaaaac cggtaagtag cgggtttaat tccgttacta aagtatcttt atattggaac
     4981 acttgcccaa agcctatatc cattctaaat ttcgattttt tggtctccat atagttggat
     5041 acgtactttt ttgcattttc ttccagtttt ttattttcgt tgtttaaatt atcctcgatc
     5101 gtcgaattga taataaacac ccgggatgtc atttctgcta tttgtgcatt gatagtgtta
     5161 atttcactaa ttaatttatt tttttcaagt gtttgataat ttgataaatt agataattcg
     5221 gttttcaaat taaaagcctc attctccagc gaatcttgtt taaatttaat agctattttc
     5281 tccattgtta aattcatatt taattgattg atgctacttg caactgcacc taacactgtt
     5341 tcataacctt tggaaaagct ggtatcggtt aatgggttgt atccaattgt tgtcttaaat
     5401 gaataggcaa attggttacc ttttgtttgg ttttcggtag atccgaatga aaattcagtg
     5461 tttgccacaa tagaatgaag gaaattcgca tcactccaac tatctattgt tttgtttttg
     5521 taaaaaagta gccagaaagg ttgacattgt agtgcaatat ttggattgag ttgagcatcc
     5581 tgaaaaaaat aatcaatttt aaaatcccgg ctaaagcccg gtgtggatac attttcaggg
     5641 ttaacaccca ataacgtaaa ggcaccggaa ctaggaattg aatattgggt agcctttaat
     5701 aaagtgtcct ttaataatgg atcccctact tgtcctttac tatttgaatg caccaatcca
     5761 acgagtaaaa aaaatattaa ataatttttc atgttattgc attaaatttt tttgaaataa
     5821 acatcgtttt gcgtagccaa tgaggaacca atatgttcta tggttatttg ttcggaaagc
     5881 gcaaccccgt tttgataaaa agtatagtgc actaatattt gagagtgtcc gtaagcagat
     5941 gcaaatgctc ccgctataac aacaacccat gagctaagtt gtgctggcac tataggtaaa
     6001 ataaatacat cttctatacc atcagaaaac gacccttttc cgattttttg catttgtgtc
     6061 ccatttgtga tataaatatc ataagcaccg tttgttgcgt aaaacggttt aagtattagt
     6121 gtaacaacac ttccttgcgg tgaaaaatag acaatgtttt ctttgtgcat attaataatt
     6181 ttaatttgtg aaataaaata caaataagcc cggtaagagg cgattttatt ctgcaaattg
     6241 aatgcaatta attagttaat acaagggtat ttttaagtta ttaaaggggt ataaatacgg
     6301 ggtttaaaac accctaactt ttggtgctta tttaactagt tttggttttg aactactaaa
     6361 catgacacaa tgaaaaatag aattacattt atccttattg cacttattat agttgctctt
     6421 tcagttttag gcacatatag ctggctcaaa cagactgccc aattaaaatc agctgaagat
     6481 gctgaacgaa ttgcggctat gaaaaaatta gaagattctt ataaaacaaa tttcgattgt
     6541 gaaaacacct ataacattat agattttaat gcacttctaa ataatttact ggcagcacca
     6601 attacc
//
LOCUS       DAORKW010000158         6597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_402_length_6597_cov_6.051819, whole genome shotgun sequence.
ACCESSION   DAORKW010000158 DAORKW010000000
VERSION     DAORKW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_402_length_6597_cov_6.051819"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1499
                     /locus_tag="PLB46_07685"
     CDS             213..1499
                     /locus_tag="PLB46_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76436.1"
                     /translation="MHPTIKFRIDSALLAEYTWTLKVFGQYIGFNVEVVTENYDIYVA
                     EHGLGDIQVSHFFRNTYESGDKQFKAYFRTSPLHYTASNKPDYLSSCFYLLSYMQEYA
                     DYYPDKYNRFPFEISVQHHYGIHRQNLVADYFDQLYLSMPKLQAIISKKERPAAFFLS
                     HDIDSVYGAFGDNYAHLIKKMRIGTLMQLLFNHYIKTPDYLLLNKIMDIEDEFDVRST
                     FFWLVNQGKGTRNIHNADYNFKEHKIQRVVNEITARGWANGLHKSAGKDTFENELKRL
                     NTSGILINRNHYLLTELPTTFEALQNAGVQIDCTMGFPENIGFRNSYGLPIQPFNFTS
                     KSPYAFIEVPLNVMDTTLKFYNGQNALQASNTIMQFLENNKTNALISVLWHNNYFFDY
                     ADTGWITTYKTILQFIRENKFEVITPEQIAATYRLL"
     gene            complement(1532..2341)
                     /locus_tag="PLB46_07690"
     CDS             complement(1532..2341)
                     /locus_tag="PLB46_07690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF76437.1"
                     /translation="MQYDPVKRSLGNVFKRTPSLRILFYKMLDLLLLRSWHIHKELKI
                     WAKGKRESEQHILDAGSGFGQYSYYLNSMSSKWKFLGVDVKEEQIADCNQFFKQIGRN
                     NIRFQVKDLTTFTIPEHFDLVVCVDVMEHVLEDVQVFKNYHTSMKPGAMLLISTPSDQ
                     GGSDVSGDGDASFIEEHVRDGYNINDIQDKLKLAGFSKTEAYYAYGNPGKISWKLSMK
                     YPLQLLNVSKLFFILIPFYYLITFPFCLILNKMDVNGKHATGTGLIVKAWK"
     gene            complement(2345..4840)
                     /locus_tag="PLB46_07695"
     CDS             complement(2345..4840)
                     /locus_tag="PLB46_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76438.1"
                     /translation="MEQNKAGKKLLTYIAIFAFFLVFSALYFSPLLQGKILLQGDIMN
                     YIGTSQEISKHYEETGEAALWTNSMFSGMPTYQIALYNSGNVFEKVNKYLGGFLPRPA
                     NYMFIALFTFFLLLKSFKVNNFLAMSGALCYALGTYLITFIEAGHNTKVNALALMPLV
                     ITGINYLLQKRYWLGGAITLFAMTCEITANHPQITYYMFLMIFCWMVSELYFAIKEKR
                     FMEFAKVAGIIVVTTLLAVSANTSKLWTTYEYSKETIRGGSHLTHQAEKQDDGLNRDY
                     AFSWSSGIAESFSIMYPNFAGGGSGKSFLQTREGEPIEGPLMSYLQSVNQQNPQKAQE
                     LLRYSGQAGKYWGEMPFTSGPIYVGAILCFLFIIGMLIGDNKIRWWLFAATVLSLFLG
                     WGKYFPAFNNFMFDYFPLYNKFRTVSMAMTILCFTIPVLGYYIMDRFLNNSNGLDDTQ
                     KLNGLKWASISVGGLSLLLMLSGSLFDLTSPAEEAYLQQVNDPQSAAFFELIKDERVS
                     MIRQDVLLATFFMGLTALILFLFLKKTVSRNIAIAVICILPVVDLLIVDSHYLTKDNF
                     VEANYYDQRFRQSVPAIKDNDPYYRVMSTMVDVDKDGSTSYLHKSIGGYHGAKLQRYQ
                     DLIEGYLSKGNVQVLSMLNTKYLYGARNGKISIERNPMAMGNAWFVDSIAIVENDDAE
                     FEGLANFDPRTKAFVHQEFKNLIPATVAAPDSTRSITLKKYGMDEISYTSKSNTDAPA
                     IFSEVWYRGNEDWKAYIDGKYVDHFRANYILRGLWVPKGEHEIVFKFEPKSYYSGKKI
                     STATSSLILLLVAVGAFFAYRNENKPTVTDNKL"
     gene            4939..6243
                     /locus_tag="PLB46_07700"
     CDS             4939..6243
                     /locus_tag="PLB46_07700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF76439.1"
                     /translation="MRKVLIITYYWPPAGGVPVQRWVKMAKYFRDFGWEPVIYTVTDG
                     EYPVLDATLEATLPDNIEVIRRPIREPYTLYKKLLGFKKGEKINAGFLHEKKKQGFMQ
                     QFAVWIRGNFFIPDARKFWIHPSILFLTKYLQSHPVEAIISTGPPHSMHLIANGVKQK
                     TGLPWIADFRDPWTKIDYYQDLHLSNWADKKHHTLEKKVLKAATKVVTVSWDWAKDFN
                     AINNGNTEVITNGFDADDFPQEIIPLFEGFVLNHIGMINKARNPEILWEALKELCDEN
                     VSFQNDLQIRFTGKYDYSVAAYLEKYQLNSKVFFAGQLTHQEATKAMMQSTVLLLLLN
                     DAQDIMGRIPAKLFEYLAAKRPVLVIGNLQGDAAKIIHETGAGIVADFKDKQAIKNAL
                     STMYKAFKNGALKADTAGIAHYSRQNITKKYAELLNRITTKQ"
     gene            complement(6309..>6597)
                     /locus_tag="PLB46_07705"
     CDS             complement(6309..>6597)
                     /locus_tag="PLB46_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HRF76440.1"
                     /translation="AFQKLIASCKFIVTDSGGIQEEATFRKKPCITIRPNTERPSTIT
                     LGTNTLVEADISKINQLITDIEKHNYKEGNIPPLWDGKATARIFQVLDKLR"
BASE COUNT         2162 a         1187 c         1158 g         2090 t
ORIGIN      
        1 catggaaagc ttatatgcgc agacaaacga atacgattaa ttattccact aatttgccat
       61 tggcgcaggg aattaaattt gatatttaaa tttcgggtga attaccattc attccaaaat
      121 ttcacatgat agtgcgacag tgttgttcat ttaagtctac tcaatggcaa cactgttttt
      181 ttatttttat aatttagcca taaatatatc acttgcatcc aactattaaa tttcgcatag
      241 attccgcatt gttggctgaa tacacctgga cactaaaagt gttcggtcag tatatcggat
      301 ttaatgtgga agtggttaca gaaaattatg atatttatgt tgccgagcat ggattaggtg
      361 atattcaggt ttcccatttt tttagaaata cctatgaaag tggcgataaa caatttaaag
      421 cgtatttcag aacttctcca ttacattata ctgcatctaa caaaccggat tatttaagct
      481 cttgttttta tttgctgagt tatatgcagg agtatgcgga ttattatcct gataagtata
      541 accgatttcc atttgaaatc agtgttcaac atcattatgg tattcaccgg caaaacctgg
      601 ttgcagatta ttttgaccaa ttgtatttat ccatgccgaa attgcaggca attatttcaa
      661 aaaaagaaag gccagctgca tttttcctat cgcatgatat cgattctgtt tacggtgcgt
      721 ttggtgataa ctatgcacat ttgattaaaa aaatgcgtat tggcactttg atgcagcttt
      781 tgtttaacca ttatattaaa acaccggatt atctgctgct gaataaaata atggatattg
      841 aggatgaatt tgatgttagg tccacatttt tttggttggt taatcagggc aagggcacaa
      901 gaaatattca taatgcagat tataatttta aggaacacaa aattcaacgg gtagtaaacg
      961 aaattaccgc tcggggttgg gcgaatggat tacataaaag tgcagggaaa gatacatttg
     1021 aaaatgaact gaaacgttta aatacatccg gtatcctcat caaccgcaac cattatctgc
     1081 ttaccgaatt acccacaaca tttgaagctt tacaaaatgc cggagtgcaa atagactgca
     1141 ccatgggttt cccggaaaat attggattta gaaatagtta tggcttacct atacagccgt
     1201 ttaattttac ctctaaatct ccttatgcat ttattgaagt accattaaat gttatggata
     1261 ctacactgaa attttacaat ggtcaaaatg cgttacaagc atcaaacacc attatgcagt
     1321 ttttggagaa taacaaaacc aacgcattga tttccgtttt atggcacaat aattatttct
     1381 ttgattatgc agataccggt tggataacta cttacaaaac tatactgcag tttattcgag
     1441 aaaacaaatt tgaagttatt acaccggaac aaattgccgc aacatatcgg ttgttgtaac
     1501 tacttattgt aaaagttaca ataagtcaac attatttcca ggcttttaca attaatcctg
     1561 ttccggtagc atgttttccg tttacatcca ttttatttaa aattaaacag aagggaaaag
     1621 tgatgaggta ataaaatgga ataaggataa aaaatagctt agaaacattc agcagctgca
     1681 aaggatattt catggaaagc ttccacgaaa ttttaccggg atttccataa gcataataag
     1741 cttcggtttt gctgaaacct gccagtttta atttatcctg aatgtcgttg atgttatatc
     1801 catcacgcac atgttcttca ataaatgaag catcaccatc gccggaaaca tctgagccac
     1861 cttgatcgga gggagtggag atgagtaaca tagcacctgg tttcattgag gtgtgataat
     1921 ttttaaatac ctgtacatct tccagcacat gttccattac atccacacaa actaccaaat
     1981 caaaatgttc aggaatggta aatgtagtta aatctttaac ctgaaaacgg atattatttc
     2041 tgccaatttg tttaaaaaac tgattgcaat cggcaatttg ttcttctttt acatcaacac
     2101 ctaaaaactt ccatttgcta ctcatggaat tcaggtaata agaatattga ccgaaccctg
     2161 agcctgcatc taaaatatgt tgttcgcttt cgcgtttacc tttcgcccaa atttttaatt
     2221 ctttatgaat atgccaagac ctgagcagga gcaggtccag cattttataa aataatattc
     2281 ttaatgaagg agttcgttta aaaacgttgc ccaaacttct tttaaccgga tcgtactgca
     2341 tgaattataa cttattatct gtaactgttg gtttgttttc atttctgtaa gcaaaaaatg
     2401 cacctactgc taccaacaat aaaattaacg atgatgttgc ggtagatatt tttttaccac
     2461 tgtaatacga cttcggctca aatttgaaca caatttcatg ttcacctttt ggcacccaaa
     2521 gaccgcgtaa aatataattt gctctaaagt ggtctacata tttgccatcg atataggctt
     2581 tccaatcttc attaccgcga taccatactt ctgaaaaaat ggcaggagca tcggtattac
     2641 tttttgaagt ataggaaatt tcatccatac catatttttt aagggtaata ctgcgtgttg
     2701 aatccggagc agcaacagtg gccggtatta aatttttaaa ttcctgatgc acaaaagctt
     2761 ttgttcgagg gtcgaaattg gcaaggcctt caaattcggc atcatcattt tcaactatgg
     2821 caatggaatc aacaaaccag gcattaccca ttgccatcgg attgcgttcg atagatattt
     2881 ttccattgcg tgcaccatat aaatatttgg tattcagcat actgagcact tgcacattgc
     2941 ctttgctcaa ataaccttca attaaatcct gataacgctg caatttcgca ccgtggtagc
     3001 caccgatact tttgtgtaaa taagatgtgc tgccatcttt atctacatct accatggtac
     3061 tcataactct gtaatacggg tcgttatctt ttatcgccgg aaccgattgt ctgaaacgct
     3121 gatcataata attggcttcg acaaaattat ctttagttaa ataatgtgag tctactatta
     3181 ataaatccac aacaggtaaa atacaaatta ctgcaatagc aatattacgg gacaccgttt
     3241 tcttaagaaa taaaaataat atcaatgctg ttaaacccat aaaaaatgtc gccaataaaa
     3301 catcctggcg aatcatactt acacgttcat cttttattaa ttcaaaaaat gcagcagatt
     3361 gcgggtcatt aacttgttgt aaatacgctt cttcggcagg gcttgtcaaa tcgaataaac
     3421 tgccggataa cattaataat aaactcaaac caccaacact aatacttgcc cattttaatc
     3481 catttaattt ttgggtatca tcgaggccgt tcgaattgtt tagaaagcgg tccatgatat
     3541 aataacctaa aacaggaatg gtaaaacata atatggtcat agccattgac accgttctaa
     3601 atttattata cagcggaaaa taatcgaaca tgaaattatt aaatgccgga aaatatttac
     3661 cccatcctaa aaacaaggat aaaacagtgg cggcaaataa ccaccagcga attttattat
     3721 ctccaatcag catgccgata ataaacaaga aacataaaat agcaccaaca taaattggtc
     3781 cggaagtaaa tggcatttcg ccccaatatt taccggcttg tcccgaataa cgtagtaatt
     3841 cctgcgcttt ctgcggattt tgttggttca cagattgcag gtagctcatc aatggtcctt
     3901 caattggctc tccttcgcgt gtttgtaaaa atgatttccc tgaaccacca ccggcaaaat
     3961 tcggatacat gatactaaat gattcggcaa taccactact ccatgaaaaa gcataatcac
     4021 gatttaatcc atcatcttgt ttttctgcct gatgagtaag gtgtgagcca ccgcgaatag
     4081 tttctttgct atactcataa gtggtccaca attttgatgt attggctgaa acggctaata
     4141 aagtagtaac cactattatt cccgcaactt tagcaaattc catgaatcgt ttttctttta
     4201 tggcgaaata caattctgaa accatccagc agaaaatcat caaaaacata taataggtaa
     4261 tttgcggatg gttggcagtt atttcgcaag tcatggcaaa caatgtaatt gctccgccta
     4321 accaatatct tttttgtaat aaataattaa taccggtaat taccaatggc attaaagcca
     4381 atgcatttac tttagtgtta tgtcctgctt ctataaaagt gattaaataa gtgcctaggg
     4441 cataacaaag tgcgccggac atggcgagaa aattattcac cttaaaactt tttagtaata
     4501 agaaaaaagt gaacaaagca ataaacatat aatttgcagg tcttggtaaa aatccgccta
     4561 aatatttatt tactttttcg aaaacatttc cactgttata caatgctatt tgataagttg
     4621 gcataccgct gaacatactg ttggtccata aggcagcttc gcctgtttct tcataatgtt
     4681 tggaaatttc ctgactggtt ccaatataat tcatgatatc gccctgtaac aaaattttgc
     4741 cttgtagcaa tggactgaaa tacaatgcgc taaaaacgag gaagaatgca aaaattgcaa
     4801 tataagttag gagtttcttt ccggctttgt tttgttccat atcaggttaa aatttgaagt
     4861 gctaaagtta agaacttcgg ggtaattggg gcaatttgtt cattcaatta tataatttta
     4921 accctttaaa cgacccaatt gaggaaggtt ttaattatta catattattg gccaccggca
     4981 ggcggagttc ctgttcaacg ttgggtgaaa atggcgaaat attttcgtga tttcggctgg
     5041 gagcctgtta tttacacagt tacagatggt gaatatcctg ttttggatgc aactttggag
     5101 gcgactttac cggataatat tgaagttatc aggcgaccaa taagggaacc ttatacgctc
     5161 tataaaaaat tattaggctt taaaaaaggg gagaagatta acgccggatt tctacatgaa
     5221 aagaagaaac agggtttcat gcaacaattt gccgtttgga taagagggaa tttttttatt
     5281 cccgatgccc gaaaattttg gattcatcct tccattctgt tcttgactaa atatttacaa
     5341 tcacatcctg ttgaggcaat aatttctacc ggccctccgc atagtatgca ccttattgcc
     5401 aatggggtaa aacaaaaaac aggtttgcca tggattgccg atttcagaga cccttggaca
     5461 aaaattgatt attatcaaga tttacatctt tcgaattggg cagataaaaa acatcatacc
     5521 ctggagaaaa aagtgctaaa ggctgccaca aaagtagtca cggtaagttg ggattgggca
     5581 aaagatttta atgctataaa taatgggaat accgaagtaa ttactaatgg atttgacgct
     5641 gatgattttc cacaggaaat aattcctttg tttgaaggtt ttgtgctgaa tcatatcggt
     5701 atgattaaca aagcaagaaa cccggaaatt ttatgggaag ctttaaaaga attgtgtgac
     5761 gaaaatgtat cattccaaaa tgatttgcaa attcgtttta caggtaaata tgattatagt
     5821 gttgcagcat atttagaaaa atatcaactc aactcaaaag tattttttgc cggtcagctc
     5881 actcatcagg aagcaactaa agctatgatg caatctactg tattattatt gttgttaaat
     5941 gatgcacaag atattatggg acgaattccg gctaagttat ttgaatattt agctgcaaaa
     6001 cgtcctgttt tagtcatagg caatttgcaa ggtgatgcag cgaaaataat tcacgaaaca
     6061 ggtgcaggaa ttgttgcaga ttttaaagat aaacaagcaa taaaaaatgc gctgagcaca
     6121 atgtacaaag cgtttaaaaa cggagcccta aaggccgata ctgcaggaat agcccactat
     6181 tcacgtcaaa atattacaaa aaaatatgcg gaattgttga accgcattac cactaaacaa
     6241 taattaattt ttaccgaaac atttaggtgc ttgcataaca tttatttaat gcaaaacgcc
     6301 gttttttttt aacgtaattt atccaacacc tggaaaattc ttgcagtagc ttttccatcc
     6361 cataatggag gaatattgcc ttctttgtaa ttgtgttttt cgatatcggt aataagttga
     6421 tttatttttg aaatgtccgc ctcaactaaa gtatttgtgc ctaatgttat tgtagaaggt
     6481 cgttcagtat ttgggcgaat agtaatgcaa ggtttttttc tgaatgtagc ttcttcctga
     6541 ataccaccac tatcggtaac aataaatttg caggaagcaa ttaatttttg aaatgca
//
LOCUS       DAORKW010000159         6593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_403_length_6593_cov_5.018048, whole genome shotgun sequence.
ACCESSION   DAORKW010000159 DAORKW010000000
VERSION     DAORKW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_403_length_6593_cov_5.018048"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..847)
                     /locus_tag="PLB46_07710"
     CDS             complement(302..847)
                     /locus_tag="PLB46_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76441.1"
                     /translation="MKKSDIYEISIKILGLYLFFTSIGLLRDVLTTFALMTEAKHNPD
                     AFGEFDQTPFLILSIANFVFVILFAAFMTLKTKTITKLVCKPTDYTETSSLFADRKVI
                     YEMALVIMGLLLIVWTLPDFVFKLKNHIELVQLNTPTNYLDTNFIITSAIKIVVGLIA
                     IIYAKSLSTILIKGSKNGQTE"
     gene            complement(883..2586)
                     /locus_tag="PLB46_07715"
     CDS             complement(883..2586)
                     /locus_tag="PLB46_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRF76442.1"
                     /translation="MKTSVFSFIFAITITCFWALPACKNNVGAEDQTGELENIPALKP
                     VFQPFTVDSQVINTFLAQFPDFNFHKAEIHQFYKNRQYQSAWFNEFGLIEQGGFFMNQ
                     LEHFEDEGLNDSVLYRAQLKTLHNIIADPNFIYNSTDTVPKQFELMLTAEFFVYAQKV
                     WFGVNEKTTQELDWYVTRKTVPSVAILDSILSGGANAFTAYEPLYPQYALLKSILKKY
                     RQISYTNWDTLKLPAEIKSLKPGDNYPVVAVIKSRLMLLGDAGILDSTTIIDSATVVA
                     IKQFQTRHGLTGDGVAGEKFFSQINISPASRIRQLEINMERMRWLPDAPTGNYIMVNI
                     PEYTMHIFENDTLAWDMKVVVGKSSSSTTIFNDELEYIVFSPYWVPPPSILNNEILPA
                     LKKDANYLKKENMEAVDPSTKKVVDVSGVDWSKYSKIPYIIRQKPGNSNSLGWVKFIF
                     PNQHNIYFHDTPSRNLFEKDARSFSHGCIRVAEPKRFATYLLRNDTTYTAAKIDSLYY
                     LGRETYVKLEEKIPVYLVYFTAWVDTKGLIHFSRDIYNHDKKLEHTLFGDAKKEEDPE
                     I"
     gene            2667..2978
                     /locus_tag="PLB46_07720"
     CDS             2667..2978
                     /locus_tag="PLB46_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRF76443.1"
                     /translation="MSDQKEKNQLNIELSEEIAEGTYSNLAIITHSSAEIVIDFIRVM
                     PGVPKAKVKSRILMTPQHAKRLLRALADNINKFESLHGTITENEGFGGLPMNFGPTAK
                     A"
     gene            2999..3742
                     /locus_tag="PLB46_07725"
     CDS             2999..3742
                     /locus_tag="PLB46_07725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF76444.1"
                     /translation="MEAKPFISVVSPVYEGEQLVNVLISRTEAALLQITDDFEIILVD
                     DGSSDNSVAVIEQIIQQHKKIKCVVLDKNYGQHIAIKAGLDNCHGNWVVVMDCDLQDQ
                     PEAIIELMKKAEEGFDAVFASRIKRNDPKIKQWYSTAFYTVLSFLSGIKTTGSTANFG
                     VYSRQVIQQICQTKYTNFFFPVAVRKATKLTANCPVDHGVRAAGNTTYTFGKALGLAI
                     KVIFSNTRLSFFQNKKPVIYGVKQFINFK"
     gene            3742..4863
                     /gene="rffA"
                     /locus_tag="PLB46_07730"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             3742..4863
                     /gene="rffA"
                     /locus_tag="PLB46_07730"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF008687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HRF76445.1"
                     /translation="MIPFNQPCLVGNELAYIADAVKRGKISGNGFYTQTCQQFLENEF
                     GYLKTLLTNSCTDALEMAALLIDIKAGDEIIMPSFTHVSTANAFLLRGAKIVFADVET
                     DTPNLDVTKIASLITKNTKAIVVVHYGGIAVNMQEVLNLAIEHNLFLIEDAAAALGGF
                     YGKVPLGSIGHLATLSFHETKNVQCGEGGLLIINDERLITRAEILWEKGTNRVAFNRG
                     EIPSYDWMDIGSSFLPSELNAAYLYAQLEQFYHIQTVRRQQWERYKDALTPIMEAGRL
                     NFISIPPYATPNYHLFGLLCANEQDRNNLAAALLKSGILAVSHYRALHLSPFFKNHYS
                     GAELPNAVSFQQRILRLPLFHDLKVEDISIICDRVKSLI"
     gene            5003..5890
                     /locus_tag="PLB46_07735"
     CDS             5003..5890
                     /locus_tag="PLB46_07735"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRF76446.1"
                     /translation="MLGIALFSLFRVNYAQPVTLSREFGANLGFSSFLGDLGGSDDIG
                     RPFFWDIDPAVTRPALGFVFRQEIIPRTAFRFNLFASMLKGDDALTDNEFRNYRNLSF
                     RSPIVEATGMIELSAFRFTGFNKKRWSPYIYGGIGAFYFNPQAQYNGKWVDLQPLGTE
                     GQGLPEYPDREKYSRIAVCFPLGGGFRILTYNHWVLGFEMACRFTTTDYIDDVSSYYP
                     NPDYYYLHYDLDKAIMAATLSNQADGSRPDLVQIEEGRGDPTNNDTYIFGGMLTFTYH
                     IEFHRKVNTIRCYFDGDNK"
     gene            complement(5897..6226)
                     /locus_tag="PLB46_07740"
     CDS             complement(5897..6226)
                     /locus_tag="PLB46_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76447.1"
                     /translation="MVSRKTGAAQLTYADINKAIIDNHTIIINCSPVGMYPDINQCPD
                     IPYQFINSNHILYDLIYNPGKTMFLQHGEKQGATIKNGLEMLELQAEYAWEIWNRVED
                     EEAASGY"
     gene            complement(6290..>6593)
                     /gene="aroE"
                     /locus_tag="PLB46_07745"
     CDS             complement(6290..>6593)
                     /gene="aroE"
                     /locus_tag="PLB46_07745"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRF76448.1"
                     /translation="KFEREEIEDCKYELYPLENIEDIRLLFEVNKDLKGLNVTIPYKE
                     SVIEYLDDLDDIAQKIGAINCIKIDEIQRVGYNTDYAGFRDSLKPLLKKHHTQALV"
BASE COUNT         2075 a         1105 c         1237 g         2176 t
ORIGIN      
        1 atcaattgaa ctggaataaa taacagttta atctgaaaat actaagcctc ctttaaccgg
       61 gaggcttttt ttattggtat gagttaaata attagttgaa agatatgtaa agcttaagat
      121 aaaaaactca ttattaatct gatgggaatg gaggattaat ttataacgtt tcgcggattt
      181 aagaagttgg cgatttcgga gcataaaact gtctgacagt gatatacttg atacgaagtg
      241 caaaagtaca cttaatcatt aaaccgtcaa tttcttacaa ccgctgtttt gtgttgccat
      301 tctattctgt ctgcccattt ttgctgccct ttattaaaat tgtcgatagg gattttgcgt
      361 aaataattgc aattagtcca acaactattt taatagctga tgtaataatg aaatttgtgt
      421 caaggtaatt ggtcggcgta ttaagttgaa caagttcaat atggtttttt aacttaaata
      481 cgaaatcagg aagtgtccaa acaattaata gaaggcccat aattactaga gccatttcgt
      541 aaattacttt tctgtctgcg aatagcgatg aagtctctgt gtagtctgtc ggtttgcaaa
      601 cgagctttgt aattgtcttg gttttaagtg tcataaacgc tgcaaataga attacaaaca
      661 caaagttggc tatactcaaa attaagaaag gtgtttggtc aaattcgcca aatgcatctg
      721 ggttatgttt tgcttctgtc atcaatgcga acgttgtaag cacgtcacgc aacaaaccaa
      781 tagatgtaaa gaataaataa agtccgagta ttttaattga aatttcgtag atgtctgatt
      841 ttttcattgt ctgttgtatt tttctttagg gttgcacatt acttaaattt caggatcttc
      901 ttcctttttt gcatctccaa ataatgtgtg ctctagcttt ttatcgtggt tataaatatc
      961 acgactaaaa tgaattaatc ctttggtgtc aacccaagcg gtaaaataaa caagatacac
     1021 cggaattttt tcttccagct tcacataagt ttctctgcct aaataataca atgaatcaat
     1081 tttagctgca gtataagtgg tatcgttgcg caataaataa gtagcaaatc gtttgggctc
     1141 tgcaacacgg atacatccat gactaaaact ccttgcatct ttttcaaata aattacgtga
     1201 tggggtatcg tggaaataaa tattatgttg attcgggaaa ataaatttca cccaacccaa
     1261 tgaattactg ttgcccggtt tttgtctaat gatatatggt attttggaat atttactcca
     1321 atcaacaccc gaaacatcaa ctactttttt ggttgatggg tcaacagctt ccatattttc
     1381 ttttttcaaa taattggcat ctttttttaa tgccggcaaa atttcattat ttaaaatact
     1441 tggcggcggc acccagtaag gactaaatac gatgtattct aattcatcat taaaaatagt
     1501 tgtgctgctt gaggactttc ctactactac tttcatatcc caggccaatg tatcgttttc
     1561 aaaaatatgc atggtatatt ccggaatatt caccatgata taattaccgg taggtgcatc
     1621 aggcaaccat cgcatacgtt ccatattaat ttctaactgg cgaatacgag atgccggact
     1681 aatatttatt tggctaaaaa atttttcacc agctacacca tcgccggtaa gaccatgacg
     1741 tgtttgaaat tgtttgatgg ctacaactgt cgcagaatca attatggtgg ttgaatctaa
     1801 aattccggca tctcctaata acattaaacg agatttaatg actgcaacca ccggataatt
     1861 atcaccaggt tttaaagatt taatttctgc aggtaatttc aatgtatccc aattcgtgta
     1921 actaatttgt cgatattttt ttaatatgga tttaagtaaa gcatattgcg gatacaaagg
     1981 ttcatatgcc gtaaatgcat ttgcgccacc ggacaaaatt gaatctaaaa ttgccacaga
     2041 tggaactgtt ttgcgtgtga cataccaatc taattcctga gtagtttttt catttacccc
     2101 aaaccacact ttttgtgcat acacaaaaaa ttctgcggta agcataagtt caaattgttt
     2161 gggtacggta tcggtactat tataaataaa attcggatct gcaattatat tgtgcaatgt
     2221 ttttaattgt gcacgatata aaacggagtc gtttaaacct tcatcttcaa aatgttcgag
     2281 ctggttcatg aaaaatccgc cttgttcaat taaaccaaat tcgttgaacc aagccgattg
     2341 atattgacga tttttataaa attgatgtat ttcagctttg tgaaaattga aatcaggaaa
     2401 ctgcgctaaa aatgtattaa tcacctgact atcaactgta aagggttgga ataccggttt
     2461 tagggcagga atgttctcta attctccagt ttggtcttcg gcaccaacat tgtttttgca
     2521 agcaggaagt gcccagaaac aggttatggt tattgcaaag atgaaagaaa aaacagaggt
     2581 tttcataccg atagtttgtg ctaaattgct aaacaatttc gatatgaggt gaatttggaa
     2641 taattttact agcaaaatat atagttatga gcgaccaaaa ggaaaaaaat caactcaata
     2701 ttgagttatc ggaagaaatt gcagaaggaa catattctaa tctagctatc ataacgcatt
     2761 caagtgctga aatagtaata gattttatcc gggtaatgcc tggtgtacca aaagccaaag
     2821 ttaaaagcag aattttaatg acgcctcaac atgctaaacg tttgttgcgc gcattagctg
     2881 ataatattaa taaattcgaa agtctccatg gcactattac cgaaaatgaa ggatttggtg
     2941 gtttgcccat gaattttgga ccaacggcca aagcttaatt cattattagc ataattttgt
     3001 ggaggcgaaa ccttttatat ctgttgtaag tccggtttat gaaggggagc aattggtaaa
     3061 tgtattaatt agccgtacgg aagcagcgtt gttacaaatt actgatgact ttgaaattat
     3121 tttagtagat gatggcagca gcgataattc agttgctgta attgaacaaa ttattcaaca
     3181 gcataaaaaa attaaatgtg tagtgttgga taaaaattac gggcaacata tcgcaataaa
     3241 agcgggactt gataattgcc atggtaattg ggttgtggta atggattgcg atttacaaga
     3301 tcagccagaa gccataattg aattaatgaa aaaagcagag gaaggatttg atgcggtatt
     3361 tgcttcccgc attaaacgga atgaccctaa aattaaacaa tggtattcca ctgcatttta
     3421 tacagtatta tcttttttat caggcataaa aacaacaggt tcaacagcta atttcggcgt
     3481 atatagtcgg caggttattc aacaaatttg ccaaacaaaa tatactaatt tttttttccc
     3541 ggtagctgtc cggaaagcaa ctaagctcac cgcaaattgc cccgtagacc acggtgtgcg
     3601 ggctgcgggc aatacaacct atacttttgg taaggcacta ggtttagcaa taaaagtaat
     3661 ttttagtaat acgcgattaa gtttttttca aaacaaaaag ccggtaattt acggtgtaaa
     3721 acaattcatt aattttaaat aatgattcca tttaaccaac catgtttagt aggaaatgaa
     3781 ttggcctata ttgctgatgc agtgaagcgg ggaaaaattt ccgggaatgg attttatacc
     3841 cagacttgtc aacagttttt ggaaaatgaa tttggttatt taaaaacatt attaacaaat
     3901 tcatgtaccg atgcacttga aatggcagct ttattaattg atattaaagc aggtgatgaa
     3961 ataataatgc catcgtttac acatgtttca actgccaatg catttttatt aagaggagca
     4021 aaaattgttt ttgcagatgt tgaaactgat acaccaaatt tagatgtaac aaaaatagct
     4081 tcgttgatta ctaaaaacac gaaggcaatt gttgttgtac attatggtgg cattgcagta
     4141 aatatgcagg aggttttgaa tttagcaatt gaacataatt tatttttaat tgaagatgca
     4201 gctgcagctt taggaggctt ttacggtaaa gtgccgttgg gcagtattgg acatttggcc
     4261 accctttcgt ttcatgaaac aaaaaatgtt caatgtggcg aagggggatt gttgattatt
     4321 aatgatgaac gcttaataac ccgtgcagaa attttatggg aaaaaggaac caacagggtt
     4381 gcatttaata ggggtgaaat cccatcttac gactggatgg acataggttc aagttttttg
     4441 ccttcagagc ttaatgcagc ctatttatat gcacaattgg aacagtttta ccatattcag
     4501 actgttcgac gacaacagtg ggaacgttat aaggatgcgc ttacacctat tatggaggca
     4561 gggagactaa attttatatc aatcccaccg tacgccactc ctaattatca tttatttggg
     4621 ctgttatgtg caaatgaaca ggatcgcaac aatttggctg cagcgctttt aaaatcgggt
     4681 attttagcgg tgtcacatta cagagcattg catctttccc cattttttaa aaaccactat
     4741 tcaggggctg aactgcccaa tgctgttagt ttccagcagc gaattctacg tttaccttta
     4801 ttccacgatt tgaaagttga agatatatcc ataatatgtg acagggttaa atcgttaatt
     4861 tgaatacggt tttaatatgt ataaaagatt aattttttaa ccaatactgg ctattttttt
     4921 gattttgtta aaaaaatata tgattttcgt ggttccctta tcattaaccc gcatgcaaaa
     4981 aaatataggt ataaagtgtt ttatgctcgg aatagctttg ttttcgctat ttcgggtaaa
     5041 ttatgctcag ccggtaacac tttcacgtga gtttggggcc aatttgggct tttccagctt
     5101 cctgggcgac ttagggggtt cagatgatat tgggaggcct ttcttttggg atattgaccc
     5161 tgcagtaacc agaccggcac tggggtttgt cttccgccag gaaattattc ccagaacagc
     5221 ttttcgtttt aatttatttg catctatgct aaaaggtgat gatgcgttaa ccgataatga
     5281 attccgcaat taccgcaatt taagtttccg cagccctatt gtagaagcga ctggtatgat
     5341 agaactaagt gcttttcgtt ttaccggctt taacaaaaaa cgctggagcc cttatattta
     5401 tgggggtata ggtgctttct actttaatcc acaggctcaa tataatggta aatgggttga
     5461 ccttcagcca cttggaacag aagggcaggg attaccggaa tatccggata gagaaaaata
     5521 ttcgagaatt gccgtatgtt tccctttagg tggtggtttc agaattttaa cctacaacca
     5581 ttgggtttta gggtttgaaa tggcttgtcg atttacgact acagactata ttgatgatgt
     5641 tagttcctat tatcctaatc ctgattacta ctatttacat tacgatttgg ataaagctat
     5701 aatggctgct acactttcga atcaggctga tggatcacga cctgatttag tacaaataga
     5761 agagggtaga ggcgatccaa cgaataacga cacttatatt tttggtggaa tgctcacctt
     5821 cacctaccac atagaattcc atagaaaggt aaacacgatt aggtgttact ttgatggtga
     5881 taataagtaa ttgaatttaa tatccggaag ctgcttcttc atcttcaacc cggttccaaa
     5941 tttcccaggc atattcagct tgtaattcca acatttccaa accatttttt atagtcgcgc
     6001 cttgtttttc tccatgttgt aaaaacatcg ttttgcctgg attataaatt aagtcgtata
     6061 aaatatgatt ggaattaata aactgatatg gaatgtccgg acattgatta atatcaggat
     6121 acataccaac cggtgagcaa tttattatga tggtatgatt atcaataatt gctttattaa
     6181 tatcagcata agttaattgt gctgctccgg ttttacgtga aaccaaagta gtgcctatac
     6241 ctaattcttg taaagcatat acaactgctt ttgaagcgcc acctgttcct caaacaagcg
     6301 cttgtgtatg atgttttttt aataaaggtt ttagtgaatc acgaaatccg gcataatcag
     6361 tgttataacc tacacgctga atttcatcaa ttttaataca attgatagca cctatttttt
     6421 gtgcgatatc atctaaatca tcgaggtatt cgattaccga ttctttataa ggaatagtaa
     6481 catttaatcc ttttaaatct ttatttactt cgaacaataa acgaatgtct tcgatgtttt
     6541 cgagtggata taattcgtat ttacaatctt caatttcttc acgttcaaat ttc
//
LOCUS       DAORKW010000160         6571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_405_length_6571_cov_4.579343, whole genome shotgun sequence.
ACCESSION   DAORKW010000160 DAORKW010000000
VERSION     DAORKW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_405_length_6571_cov_4.579343"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PLB46_07750"
     CDS             complement(<1..168)
                     /locus_tag="PLB46_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRF76449.1"
                     /translation="MAKSKLTIKPLADRVLVQPSTPETVTKGGIIIPDTAKEKPQKGI
                     VVAAGPGKKDEP"
     gene            530..1831
                     /locus_tag="PLB46_07755"
     CDS             530..1831
                     /locus_tag="PLB46_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76450.1"
                     /translation="MRTLLIVMACLTTCTAFAQIQCSNDSTGLIPLVDLGPGYYMGHQ
                     GGLFPSGSNTIPLAHKKKGIGISKALKPLDTLGNVNYEEGKIIFLGLGASLASNAFNA
                     YIDSVKIYDFEGMNNCLDVKGLFFGGKDLDQMIDFENNSYWESVNDKMISRGDTYEQV
                     QVIWVLQQSFEDTTSEFNVYYDSVINKFVTLMQVLKDTFPNLKQVFLSGVHYMGYMDP
                     LHFRYDAFVEPHGYWGNIVIKELIAKQIMGDPALRYTGPHPNAAWISWGPNFWADGKN
                     PRAYDGLSWNCDQFRDDETGGGFHLEDSVNSLGFEAQMLKSFFSFDAVTTIWHQDGPL
                     WALCPEDTLRESNIPNKTNSLLIYPNPSIGDIVIKNSIDMVGEIHIKIANEMGVMVWE
                     DVQSVQHSNYITLQLDLPNGMYFIQVQNANQNWTQSLVIQD"
     gene            complement(2319..3626)
                     /locus_tag="PLB46_07760"
     CDS             complement(2319..3626)
                     /locus_tag="PLB46_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRF76451.1"
                     /translation="MLIQFANNTFTPVMLFDTLPLAERLRPRNLDEYIGQQHLVGEGQ
                     VLRKMITGGKLVSMIFWGPPGIGKTTLANIIANAANLPFFSLSAVASGVKDVKEVIEK
                     AKNSGKIVLFIDEIHRFNKAQQDSLLGAVEKGLIILIGATTENPSFEVINALLSRCQV
                     YVLQEFTKEDFELLIERAITKDVVLSKRKIVVKENEALIKLSGGDARKLLNLLELVEE
                     TIDTDPIEITNDLMTSLAQKKTAAYDKNGENHYDIISAFIKSMRGSDPNAAMYYLVRM
                     IEGGEDPKFIARRMLILASEDIGNANPNALLLATSCFQAVTMLGYPECAIPLSQTVIY
                     LATSPKSNASYVALTNTFELVRNTGDLPVPLAIRNAPTKLMKQLNYGKDYQYAHNNPG
                     NFADMEFLPEAIKGKKVFEPGNNAKENEIRKFLRERWKDKYGY"
     gene            complement(3716..4489)
                     /locus_tag="PLB46_07765"
     CDS             complement(3716..4489)
                     /locus_tag="PLB46_07765"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF008877.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HRF76452.1"
                     /translation="MENSLLTEKMTQDYTKYTPEHFKVWEILFDRQMALLKDRAATVF
                     MEGIEAIHFHANAIPKFDEFNIRLETITGWNAVVVPGLINNKEFFELLKNRKFPASTW
                     LRSMEQLDYLEEPDMFHDVFAHLPLLTNKTYCAYLYGLSKIALSYIDNPVAVELISRI
                     YWYTVEFGLIRDNGLLRIYGSGILSSQGESIYCLKSGIPSKRIDYNVEKILDTPYIKD
                     KFQAQYFVIDSCLDLFESLPDIEQGIIKRLEHPELFVEG"
     gene            complement(4796..5086)
                     /locus_tag="PLB46_07770"
     CDS             complement(4796..5086)
                     /locus_tag="PLB46_07770"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HRF76453.1"
                     /translation="MKKSNEQGIGAAIKQLFKSYRLDEKVAEVQIKELWGKIMGVSIK
                     NYTTNMHLHKGVLTIYIESAPLKQDLKFSKDTIIQRINEELKENVVREVVIR"
     gene            complement(5096..6214)
                     /gene="recF"
                     /locus_tag="PLB46_07775"
     CDS             complement(5096..6214)
                     /gene="recF"
                     /locus_tag="PLB46_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRF76454.1"
                     /translation="MLLQRLNLLNYKNYGTITVSFEHKFNLINGLNGSGKTNLIDSIH
                     YLCLCKSYFTRSDNNVVKHGEPYFRVDGFFEMAGKSTQITGKITGVANSGGTGVKKEF
                     FRDGEPYDRLSDHIGTIPVVMIAPGDIAIINDGSEERRRFLDTAIAQVNREYLTRLMH
                     YNKLLLQRNALLKQNLMLGHLDATLMEVLNNQLAIDGDYIHQERKIYLEQFSKVFGEH
                     YYQISGENELGEVIYLSQANEKRHLELFAESKRDDIAAGRTTSGIHKDDLRLTVNTHP
                     VRETGSQGQIKSFLIALKITQCKLMQHLKGKNSILLLDDVFEKLDKKRLATLFTIFKE
                     PVFEQVFITDADEKRSSEFCAAAFNNFGHFHVHDNIIE"
     gene            6277..>6571
                     /locus_tag="PLB46_07780"
     CDS             6277..>6571
                     /locus_tag="PLB46_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF76455.1"
                     /translation="MDRSKITKETYLHWYELMYKMRRFEEKAGQMYGMQKIRGFCHLY
                     IGQEAVAAGTISAIRHEDPIITAYRDHGLAIAKGVSCNEAMAELYGKVTGCTKG"
BASE COUNT         1963 a         1266 c         1228 g         2114 t
ORIGIN      
        1 cggctcgtct tttttaccgg gaccggctgc tacaacaatt cctttttgag gtttttcttt
       61 cgctgtatca ggaataatga taccaccctt tgtaacagtt tcaggggtag aaggctgaac
      121 caacacacgg tcagctaatg gtttaatcgt taatttactt tttgccatat tgattgattt
      181 aatcgtttaa gaagttatgt aatccgcccc tgtcataaat tgtgcctttc cctactttgg
      241 gctattttgt cggataattt gggctatttt ggcagttatc atgccgattt aggctatttt
      301 cactaagatt ctgccatttg tgttaatgtg acattttggc aatataacag cttgaagcag
      361 attggaatag agaaaaccgc taaacaggta gtcttcctca attaccttac taaatgggga
      421 accttgttta aaatttactt caacaccacc acgaaaacat gtattattgt caagccaaaa
      481 gccattgtcg tgatggaatt ggcccatttt ataaacaaac acacccacaa tgcgcacact
      541 tttaattgtt atggcatgcc tgacaacctg tacggcattt gcacaaattc aatgttccaa
      601 cgattcaacc ggtttaattc cgcttgtaga tttaggtccc ggctactata tgggccatca
      661 gggcggattg tttcccagtg gcagtaacac cattccactt gctcataaaa aaaagggaat
      721 tggtatatcc aaagctctta agcctctcga tactttaggg aatgtaaatt atgaagaagg
      781 gaaaattatt tttttaggat taggggcgtc tttagcttcc aatgcattta atgcttatat
      841 cgattctgta aaaatttacg actttgaagg catgaacaat tgtctcgatg taaaaggatt
      901 attttttggc ggtaaagatt tagatcagat gattgatttc gaaaacaaca gttattggga
      961 aagtgtaaac gataaaatga tttctcgagg agatacctat gaacaagtgc aggtaatttg
     1021 ggtattgcaa caaagttttg aagatactac ttccgaattt aatgtgtatt acgatagtgt
     1081 catcaataaa tttgtcacct taatgcaagt attaaaagat acatttccca atttaaaaca
     1141 agtgttttta tcgggtgtgc attatatggg ttatatggac ccattacatt ttcgttatga
     1201 tgcatttgtt gaaccacatg gatattgggg aaatattgtg ataaaagaat taatcgcgaa
     1261 acaaataatg ggtgatcccg cgttgcgtta tacagggcca catcccaatg cagcatggat
     1321 aagctgggga cctaattttt gggcagatgg taaaaatcca agggcttatg atggtctatc
     1381 ctggaattgc gaccaatttc gtgatgatga aaccggaggt ggatttcatt tggaagattc
     1441 agttaattca cttggttttg aagcacaaat gctaaaatcc tttttttctt ttgatgcggt
     1501 gacaactatc tggcatcagg atggtccgct gtgggctttg tgtccggagg atacattaag
     1561 agaatcaaac ataccaaata aaacaaattc gttgctcatt tatccaaatc cttcaattgg
     1621 cgatattgtg ataaaaaata gcatcgacat ggttggtgaa attcatatta aaatagcaaa
     1681 tgaaatgggg gtaatggttt gggaagatgt acaatctgtg cagcattcga attatatcac
     1741 attacaactc gatttaccga atggcatgta ttttattcag gttcaaaatg ctaatcaaaa
     1801 ctggacacaa tcgttggtga ttcaagattg agcagttgat gaatttattt tttaattttt
     1861 tttacgccgg gaaggaagga agaaaggaag ttgcgggggg tttattgatg taagatgtgg
     1921 tttgtttttg agagcgcgga gctgttctcg ctaagtcgcg aagttttttg attatatgat
     1981 gtgtgatatg gattgttttc aggcgcgcaa agctgggaaa ccaatttaat ttttcattga
     2041 tgttatatta ttaattttcc gctatctctc ttttccgcgt gaaactttta tcaactggtt
     2101 cacgcggagg tgcggagaag tttattgtat ggtgtaagat gtggtttgtt tttgagagcg
     2161 cggagctgga aaaccaattt ctgctttgcg tgcttgaaat ggtttaaaat atacaataaa
     2221 caattcactt tgcgccttag cgcttttagt ccgctgaggc gggagaaacc atacgaaaaa
     2281 ttaaattgcc aacgccaaat tcaaatcata caacttaatc agtacccata tttatccttc
     2341 catcgctcgc gcaaaaattt gcgtatttca ttttctttgg cattattgcc gggttcaaat
     2401 actttttttc ctttaatcgc ttcaggcaaa aattccatgt ccgcaaaatt gccgggatta
     2461 ttatgcgcat actgataatc tttaccataa tttaattgtt tcattaattt ggtaggcgca
     2521 tttctaattg ccaaaggaac aggtaagtca ccggtattgc ggactaattc gaaagtatta
     2581 gttaatgcaa catacgaagc attactttta ggtgaagtag ctaaataaat aactgtttgc
     2641 gacagcggaa tagcacattc aggatatccc aacatcgtca ctgcctgaaa acaacttgtc
     2701 gccagcaaaa gtgcattggg attggcattg ccgatatctt cactggctaa aattaacatc
     2761 cgtcttgcga taaatttcgg gtcctctcct ccttcaatca ttcgcactaa ataatacatc
     2821 gcagcattag ggtcgctgcc acgcatactt tttataaatg cagaaatgat atcgtaatga
     2881 ttttctccgt ttttatcgta agcagcagtt tttttctgtg ccagagaagt cattaaatcg
     2941 ttggtaattt caattgggtc ggtatcaata gtttcttcaa ctaattcgag taagtttaat
     3001 aatttacgtg catctccacc acttaattta attaatgcct cattttcttt taccacaatt
     3061 tttcgtttcg ataacaccac atcttttgtg atagcccttt caattaataa ttcgaaatct
     3121 tctttggtaa attcctgtaa aacataaacc tgacatctcg acaacaatgc attaattact
     3181 tcaaatgaag gattttcggt tgttgcgcct attaggatga tgagtccttt ttcaacagca
     3241 cccaataacg aatcctgttg tgccttattg aaacgatgaa tttcatcgat aaacaaaaca
     3301 attttccccg aattttttgc tttttcaatt acttccttca catccttaac accggaagca
     3361 acagcactca atgaaaaaaa tggcagattg gcagcattgg caataatatt tgccagtgta
     3421 gtttttccaa ttcccggagg cccccaaaaa atcatactca ccagtttacc accggtaatc
     3481 attttgcgca gcacttgacc ctcaccaacc aaatgttgct gaccgatata ctcgtccaaa
     3541 ttgcgcggac gcaggcgctc agctaaaggg agggtatcga ataacatgac aggtgtaaag
     3601 gtattattag caaattgaat taacaaacgg ttgacggctt aacgcccata gttaatccgg
     3661 gtttcaaaaa aaacctaaaa tcagacttac taaaaatgaa aagggaagtg cgtttttatc
     3721 cttccacaaa cagctccgga tgttccaaac gtttgataat tccttgttca atatccggca
     3781 gactttcgaa taaatcgagg caagaatcaa tcacaaaata ctgtgcctgg aatttatctt
     3841 tgatataagg agtatctaat attttttcaa cgttgtaatc gatacgtttt gaaggaatac
     3901 ctgattttaa acaataaata ctttcacctt gtgaggataa aatacctgaa ccgtaaatgc
     3961 gcaataaacc attgtcgcga attaatccga attctaccgt gtaccaataa attcttgaaa
     4021 ttaattcaac tgcaacggga ttatcaatat aacttaatgc aattttactt aaaccatata
     4081 aataggcgca ataggtttta ttggtgagca aaggtaaatg cgcgaataca tcgtgaaaca
     4141 tatccggctc ttctaaataa tccaattgct ccatggagcg caaccaggta gaggcaggaa
     4201 attttctatt ttttaataat tcgaaaaact ctttgttatt aattaaaccg ggaacaacca
     4261 ctgcattcca tccggtaatc gtttctaatc tgatattaaa ctcatcgaat ttaggaattg
     4321 cattagcgtg aaaatgtatc gcttcaatcc cttccataaa aacagttgcc gccctatcct
     4381 tgagcaatgc catctgacga tcaaacaata tctcccaaac tttaaagtgt tcaggtgtat
     4441 attttgtgta atcctgggtc attttttctg tgaggaggct gttttccatt tcttcgttaa
     4501 tatttatgct aatatacggt gtttaggcta tggttgcatg catcacatgt taatatgata
     4561 gcaatattaa caaaggttgc acattttgga aatgaaggac gaagttgatt tttgcgggtt
     4621 aatgtgtttg aaaaggaaca cggatttaac ggatttgacg gatggggacg gatttattat
     4681 ggattgatta aaaacacagt ggacttatat ttgaaattta tttaaaccct aacaaaaaaa
     4741 tttacccgtt taaatcagtt aaaccagtta aatccgtgtt ccttttcccc acgtttcacc
     4801 gtatcaccac ctctctcacc acattttcct tcagctcctc attaattcgc tgaataatgg
     4861 tgtcttttga gaattttaaa tcctgcttta aaggtgcgga ctcaatgtag atggtgagca
     4921 cacctttatg taaatgcata ttggtggtat aatttttaat acttacaccc attatttttc
     4981 cccacaactc tttaatctgc acttcagcca ctttttcgtc taagcgataa ctcttaaaca
     5041 attgtttaat ggctgctcct atcccctgct cgttgctttt tttcatggct taaagttatt
     5101 caataatatt atcgtgaacg tggaaatgac caaaattatt aaatgcagca gcacaaaatt
     5161 cactactgcg tttttcatcc gcatctgtaa taaaaacctg ctcaaataca ggttctttaa
     5221 aaatggtgaa taatgtcgcc aaacgttttt tatccagttt ttcaaaaaca tcatccaaca
     5281 acaaaatact atttttcccc tttaaatgtt gcatcagctt gcattgtgtt attttcaagg
     5341 caattaaaaa cgattttatc tgaccctggc tgcctgtttc gcgcacagga tgcgtattaa
     5401 ccgtcaggcg caaatcatct ttatgaattc cggatgttgt tctacccgca gcaatatcgt
     5461 cgcgtttcga ctcagcaaac agttccaaat gccttttttc gttggcttgc gaaaggtaaa
     5521 tcacttcacc aagttcattt tctcctgaaa tctgataata gtgttcgcca aaaaccttac
     5581 tgaattgttc taaataaatt ttccgttcct gatggatata gtcgccgtca attgccaatt
     5641 gattattcaa cacttccatg agggtggcat cgagatgacc taacatcaaa ttttgcttta
     5701 aaagggcgtt gcgctgcaac aacaatttat tgtaatgcat cagacgagtt aggtattcac
     5761 ggttaacctg cgcgattgca gtatccaaaa acctacgcct ttcctcactt ccgtcgttta
     5821 tgatggcaat atcgccgggc gcaatcatca ccacaggaat tgtcccaata tggtccgaaa
     5881 gcctgtcgta aggctcacca tcgcgaaaaa actccttctt tacacctgtt cctccggaat
     5941 tggcaacacc ggtgattttg ccggttattt gggtagattt tcctgccatt tcaaaaaatc
     6001 catctaccct aaaataaggt tcaccgtgtt ttaccacatt attatcagaa cgagtgaaat
     6061 aacttttgca caaacataaa tagtgtatac tgtctattag attggtcttt ccggagccgt
     6121 ttaagccatt aatcaggtta aatttatgct caaacgacac agttatggtg ccgtagttct
     6181 tataattgag taaattgagt cgctgtagta acactgcttg ttttgattgt ggtctgcttt
     6241 tcttaatttt gccgttcgat ttttcgaagg atattcatgg accgctcaaa aataacaaaa
     6301 gaaacctacc tgcactggta tgaactgatg tataaaatgc gtcgctttga ggaaaaagca
     6361 ggccaaatgt atggcatgca aaaaatcaga gggttttgcc atttatacat tggtcaggaa
     6421 gctgttgctg caggaaccat cagtgccatc aggcatgagg acccaatcat aaccgcttat
     6481 cgcgaccacg gattggcaat tgctaagggc gtttcctgca acgaagctat ggctgaattg
     6541 tatggaaaag ttaccggctg caccaaaggt a
//
LOCUS       DAORKW010000161         6527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_408_length_6527_cov_5.868974, whole genome shotgun sequence.
ACCESSION   DAORKW010000161 DAORKW010000000
VERSION     DAORKW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_408_length_6527_cov_5.868974"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLB46_07785"
     CDS             complement(<1..416)
                     /locus_tag="PLB46_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRF76456.1"
                     /translation="MAIQSTNFKFPGTTEVYHGKVRDVYTIQNNVLVMVASDRISAFD
                     VILPKPIPYKGQVLNQLAAYFLEATRDVAPNWLISTPDPRVAIGYRCEPVKVEMVIRG
                     YLSGHSWREYKSGKRIICGVTMPDGLKENDKFPEPI"
     gene            complement(440..3040)
                     /locus_tag="PLB46_07790"
     CDS             complement(440..3040)
                     /locus_tag="PLB46_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76457.1"
                     /translation="MSKTKLWGIIGGGALLVFFTVYMLFFNKRNETVLNAMPNDVLCF
                     FDIKKANDFSIALNQNEIFGALRQTSMIGTLHSDFNLYAGVLSTNPELLSDLLNSRVV
                     AGAFTTGNTKIDYLILLQLDEAAKLNLKNFKPVINQQNPDINTHTFERSLIYEISYKG
                     TDTAFSFALENGIFIFSTTSVLVENAILQLKKGNPVTENEAFQDVFSNLNNASNNNYA
                     FYINTAQMADYFSMFSTNEKYASIMQMKNYVSWIGLQPELKTNGIELNGYASAKGEQS
                     TLSLGQYTGQLATDMHPAVPDNVAVLYRINSTQLSENFASKLKDESANREFFDYWSPW
                     MAESLIVGLSETLDGNLGKRAFVIVPTLDMQLAKSKLKYALVSDTLSYKGIDISTFNC
                     FEMAEKLSGIQLPEVCYGFWQNDALVIAFDYLQITNLIDAATGNRGLSKNAQYLDFKK
                     EISASFNASVYLDLAKSEKLITGYIADKHLDSLSANLQLLKQFSQLELQFTQNKNVYL
                     VNGFVHFETNPQKSSGSLWKIDLDAPVQSGPFCVYNDVSRQHCVLVQDTANQLIQISL
                     NGDVQWKLSLDSKILSDIHEVDFYNNDKTQFVFNTQNAIYLVDAQGEAVEGFPITLTT
                     RINNAIAVIDKDKENYKMFVACDNGNIYGYYKDGKPIAGWSPLKGAGIVNSSIFDFTS
                     KGNQVFGFVNDKGIQLKKENGNSLQVIPLKSAFADMIQAGNQIGVIDQMGNIIVVDSL
                     LQVQSIPGKTISGKQLILDVQHDDTLDVIYFDESYFKANTILGNNIFVTEAALAVDVP
                     VKYTAGSNTYFGYNTLDNRLFLFDADGKVINGFPIEGSAKTTIKTISSNGDKMLITVI
                     GNAVLAYRIL"
     gene            3118..3519
                     /locus_tag="PLB46_07795"
     CDS             3118..3519
                     /locus_tag="PLB46_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HRF76458.1"
                     /translation="MNQNKIIGTAAVLGALSVAIGAFGAHGLEGLVDAGKLTEQDLKT
                     FETGVRYQFYHTFALLAVGLGYHLLNAKMAKLATTLLIAGIIIFSGSLYLLVLSEPLL
                     GTRLSWLGAITPLGGLSFIAGWFFLFRATKK"
     gene            complement(3588..6242)
                     /locus_tag="PLB46_07800"
     CDS             complement(3588..6242)
                     /locus_tag="PLB46_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF76459.1"
                     /translation="MKKIFAILTFVLLAGSSFAQTSNPELDKMNYTRKMMYGDALMSA
                     GSYYNAQDVYKMAYDDDATQEAAYKLARAYYLARNYKDAEKWYKTAVEAGKSKDMYPE
                     AGFYYAMSLKYNGKYAEAKEAFKAVTKNSKLKGPIGTALKRQSKNEDKGCDLAIELGK
                     KPADVNVEHLGSKVNNNYTDFSPKYSPDNNLTYASLVSENVIEMGTQQKTELRARIFE
                     SKADGDSWAQATELKASFNKGDAHTGNGAYSPDGKRFYYTECRPNDSLVMVCKIFVSE
                     WSAGEWENGKEVGQVNEPDFTSTHPAVGTFKGKEYLYFSSNRTGGRGGMDIWYSEIQN
                     KGKNYTNPINCGTKINTTGDEITPYFNEEQQMFYFSSNGQINIGGFDIYKSKGGMKSF
                     ESPQNLGLPFNSSVDDFYFSTDKSGKKGFFVSNRKGGFSVKSETCCDDIYAYKYPPEF
                     VIMARAIDEVTKEPIMGASIDLMRSGAKMDSSVTKTANNYEDFFLGTAQDKFELTAYR
                     EKYKNGKATTSTVGLKENDTLFIDILMEPIIEMKPIEVKNIYYTLGKWDLRPESFKAL
                     DSIYNVLQLSPQLKVEIGSHTDSRDTDEKNKILSQHRADTVVSYLISRGVVAERLVAV
                     GYGESVPKTLSSEVKLPGGGTVPANTVLVESFIQKYKGEDYEYLHQLNRRTEFTIIGV
                     IPNAIIKYDEATIEANEARKREEEAKRAKQLQDLQQVDETDVIETGDIETKPNTNTNT
                     GNKGTKTTGTTPAASALDATLTKKGNMFEGNASVNGKVQTKYILNLSPSITVAMVGRD
                     YFIKLWEAGAITEADIKNDKPTDLGNGVTVKGDVFTIKTLQLGDITLTNVDTKINMTS
                     TQNLIVGVKVAEKGGCEFDAAKAKFKCK"
     gene            complement(6288..>6527)
                     /locus_tag="PLB46_07805"
     CDS             complement(6288..>6527)
                     /locus_tag="PLB46_07805"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF76460.1"
                     /translation="NANFPATFFLGGAYRLDDAIIASVGMDFKNFRFGASYDINTSTL
                     KEASSGNGGFELSLVYTGCILPVIPTHYVMPCPRY"
BASE COUNT         1979 a         1256 c         1051 g         2241 t
ORIGIN      
        1 attataggtt ccggaaattt gtcgttttct tttaatccat caggcattgt taccccgcat
       61 ataatccgtt ttccgctttt gtattctctc cacgaatgtc cgctcaagta tcctcttatt
      121 accatttcca ctttaacagg ttcacatctg tatccaatag caacacgcgg atcgggggtt
      181 gatatcaacc aatttggagc aacatctctt gtagcctcta aaaaataagc tgctaattga
      241 tttaacacct gacctttata tggaataggt ttgggtagaa taacatcaaa tgccgaaatc
      301 cggtcacttg cgaccattac caatacatta ttttggatgg tataaacatc tcgtactttg
      361 ccatgataca cttcagtggt gccgggaaac ttaaaattgg tggattgaat tgccatattg
      421 gattgttaac gcgacaaagt tacaaaatcc tatacgcgag tacagcgttg ccaattacgg
      481 ttatgagcat tttatcccca ttcgacgaaa ttgtctttat ggtagttttg gcacttcctt
      541 ctattggaaa tccattaatt accttgccat ccgcatcaaa taagaatagt ctgttgtcca
      601 gtgtgttata accaaagtag gtatttgatc ctgctgtata tttaacaggc acatcaactg
      661 caagtgcagc ttctgtaaca aaaatattat tgcctaaaat agtatttgct ttaaaatacg
      721 actcatcaaa ataaataaca tctaatgtat catcgtgctg aacatccaaa atcaattgct
      781 tccctgatat agttttaccc ggaatggatt gcacttgaag taaagaatcc acaacaataa
      841 tattccccat ttggtcaatc acgccaattt gattaccggc ttgaatcatg tcggcaaatg
      901 ctgattttaa aggaataacc tgcaaactat ttccattttc tttttttaat tgaattcctt
      961 tgtcatttac aaatccaaat acctgattac cttttgaagt aaaatcaaaa atcgaagagt
     1021 tcactattcc agcacctttt aatggactcc aaccagcaat tggtttacca tctttatagt
     1081 aaccatatat attgccatta tcacacgcaa caaacatttt ataattttct ttgtccttat
     1141 caattactgc aatagcatta ttaatacggg tagtaagtgt tatgggaaac ccttcaactg
     1201 cttcgccctg cgcatctact aaataaattg cattttgggt attaaataca aattgcgttt
     1261 tgtcgttatt ataaaaatca acttcatgga tatcacttaa tattttactg tctaaactaa
     1321 gtttccattg cacatctcca tttagagaaa tttgaataag ttgattggct gtgtcttgca
     1381 ccaacacaca atgctgacgc gacacatcat tataaacaca aaatggacca ctttgaaccg
     1441 gagcatctaa atctattttc catagtgacc cactactttt ttgaggattc gtttcaaaat
     1501 gcacaaaacc atttaccaga taaacatttt tgttttgagt aaactgcaat tccaattgtg
     1561 agaactgttt caataattgc aggttcgcag aaagcgagtc aagatgttta tcggcaatat
     1621 aaccggttat taatttttca ctttttgcta aatcaagata aacagacgca ttaaatgagg
     1681 ctgatatttc ttttttaaaa tcaaggtatt gtgcgttttt actaaggcca cggttacctg
     1741 ttgcggcatc aatcaaattt gttatttgca aataatcaaa agcaattact aatgcatcat
     1801 tttgccaaaa tccataacac acctcaggta attgtatgcc ggaaagtttt tcagccattt
     1861 cgaaacaatt gaaagtggaa atatctattc ctttataact cagtgtatct gacactaatg
     1921 catacttcaa tttacttttt gcaagctgca tatccagggt tggaacaatt acaaatgcac
     1981 gtttacctaa gttaccatct aaggtttcag acaatccaac aattaacgac tctgccatcc
     2041 atggcgacca gtaatcaaaa aattcacggt tcgcactttc atctttcaat tttgatgcaa
     2101 aattttctga caattgggtg ctattaattc tatacaatac agcaacatta tccggtactg
     2161 caggatgcat atctgttgca agctgaccgg tatattgtcc caatgataaa gttgattgtt
     2221 caccttttgc cgatgcatat ccatttagtt caataccatt tgtttttaat tcaggctgta
     2281 aaccgatcca tgaaacataa tttttcattt gcataatact tgcatacttt tcatttgtag
     2341 aaaacatgga aaagtagtcc gccatttgtg cagtattaat ataaaacgca taattgttgt
     2401 ttgaagcgtt atttaaattt gaaaacacat cttgaaatgc ttcattttcg gttacagggt
     2461 tacccttttt taattgcaag atggcatttt caacaagtac cgaagttgta ctaaaaataa
     2521 aaatgccgtt ttctaaagca aatgaaaaag ccgtatcagt ccctttatag gatatttcat
     2581 aaatcaagga cctttcaaat gtgtgggtat ttatatccgg attttgttgg ttaattaccg
     2641 gtttgaaatt tttaagattc aactttgcag cttcatcaag ttgcaataaa attaaataat
     2701 caattttggt attacctgta gtaaatgcac ctgctaccac tctgctgttc aataaatcgc
     2761 tgagcagttc tggattggta gacagcacgc cggcatacaa attaaaatca ctatgtaaag
     2821 ttccaatcat ggaagtttgc ctgagcgctc cgaatatttc attttgattt aatgcaatag
     2881 aaaaatcatt cgcttttttt atgtcgaaga aacacaatac atcattaggc atagcattca
     2941 gcactgtttc gttgcgttta ttaaaaaaca gcatataaac agtaaaaaac accaataacg
     3001 cgcctccacc tataataccc caaagctttg ttttgctcat aattcctttg ttacctttgc
     3061 aaagctaacc tgataatagg gttaatggta ttcacctatt cacatcaatt tataaaaatg
     3121 aatcaaaata aaattattgg aactgcagct gtgctcggag cattgtctgt agccattggt
     3181 gcttttggtg ctcatggact ggaaggactt gtagatgcgg gaaaattaac cgaacaggac
     3241 ctaaaaactt ttgaaaccgg agttcgctat cagttttatc acacctttgc ccttttggca
     3301 gttggcttgg gttaccacct gctcaatgca aaaatggcga aacttgcaac tacactgtta
     3361 attgccggca ttatcatctt tagcggaagt ttatatctat tggtattatc agaaccactt
     3421 ttgggcacca ggttgagttg gctgggggcg ataacaccat tgggtggcct ttcatttatt
     3481 gccggttggt tctttttatt ccgggcaaca aaaaaataac agaaaatggt ataacaaaaa
     3541 aagcgatcca aattaatgaa tcgctttttt ttataatgtt gatttgctta tttgcattta
     3601 aatttagctt tggctgcatc gaattcacaa ccgccttttt ctgccacttt aacaccaaca
     3661 attaaatttt gagtactggt catattgatt ttagtatcaa cattagttaa agtgatatca
     3721 cctaattgta atgttttaat cgtaaacaca tcacctttta cagttacacc attacctaag
     3781 tcggtaggtt tatcattttt aatgtctgct tcagtaattg ctccggcttc ccaaagttta
     3841 atgaagtagt cacgaccaac cattgcaact gtaatgcttg ggctaaggtt taagatatat
     3901 ttggtttgca ctttaccatt tacagatgca ttaccttcaa acatattacc ttttttggta
     3961 agtgttgcgt ctaaagctga agcagcagga gttgtacctg ttgttttagt tcctttattg
     4021 cctgtgttgg tatttgtatt tggcttagtt tctatatctc ctgtttcaat tacatcagtt
     4081 tcatcaactt gttgtaaatc ttgcaattgt ttagcgcgtt tagcttcttc ctcacgttta
     4141 cgtgcttcgt ttgcttcgat agtggcttcg tcgtatttaa taatagcgtt tggtattaca
     4201 ccgatgattg taaattcagt acgacgattt aattggtgta gatattcata atcttcacct
     4261 ttatattttt ggataaatga ttccactaat accgtgttag caggaacagt tccaccgcca
     4321 ggtaatttta cttcagatga taatgtttta ggtactgact caccataacc aacggcaact
     4381 aaacgttctg caacaacacc tcttgaaatc aaataactaa caactgtatc agcacggtgc
     4441 tgagaaagta ttttattttt ttcatcagta tcacggctat cggtatgtga accaatttca
     4501 acttttaatt gtgggctcaa ttgtaataca ttataaattg agtcaagtgc tttaaatgat
     4561 tcaggacgta aatcccattt tcctaatgtg taataaatgt tttttacttc aataggcttc
     4621 atttcaataa ttggctccat caagatgtca ataaacaaag tatcattttc ttttaagcca
     4681 acggttgaag tagttgcttt accattttta tatttttcgc ggtaagcagt caattcgaat
     4741 ttatcttgag ctgttcctaa aaagaaatct tcgtaattgt tagcagtttt agtaacagat
     4801 gagtccattt tagcacctga acgcattaaa tcgatagagg cacccatgat aggttctttt
     4861 gttacttcgt cgatagcacg tgccataatt acgaactctg gtggatattt atatgcataa
     4921 atatcatcac aacatgtttc gctttttaca gaaaaaccac ctttgcggtt agaaacaaaa
     4981 aatccttttt taccggattt atcggtagag aaataaaaat cgtctactga gctgttaaaa
     5041 ggtaaaccaa ggttttgtgg actttcaaaa gatttcattc caccttttga tttataaata
     5101 tcaaatccac caatattaat ttgaccgtta gagctgaaat aaaacatttg ttgttcctca
     5161 ttaaaatatg gcgttatttc atctccagta gtattaatct ttgttccgca gttgatagga
     5221 ttggtataat ttttcccttt attctgaatt tcagaatacc agatatccat accaccacgt
     5281 ccgccagttc tattagaaga aaaatataaa tattctttgc ctttaaatgt tccaacagca
     5341 ggatgagttg atgtaaaatc aggttcattt acctgtccaa cttctttacc attttcccat
     5401 tcgcccgcag accattcact cacaaatatt ttacatacca ttactaatga atcgtttgga
     5461 cgacattcag tataataaaa acgtttgccg tccggagaat atgcaccgtt accggtatgc
     5521 gcatcacctt tattgaaaga tgcttttaat tccgtagcct gtgcccaaga atcgccatct
     5581 gcttttgatt caaaaatacg tgcacgcaat tctgtttttt gttgcgttcc catttcgata
     5641 acattttcag acaccaatga tgcataagtt aaattgttat ccgggctgta tttaggtgaa
     5701 aaatcggtgt aattattatt cactttagaa cctaaatgtt caacgttaac atcagcaggt
     5761 tttttgccta attcaattgc taaatcgcaa cctttgtctt cattttttga ttggcgtttt
     5821 aatgcagttc caattgggcc ttttagtttg ctgtttttag taacagcctt aaatgcttct
     5881 tttgcttcag catatttacc attgtatttt aaactcatgg cataataaaa tcctgcttca
     5941 gggtacatat cttttgattt ccctgcttca actgcagttt tgtaccattt ttcagcatct
     6001 ttataattac gtgccaaata atatgcacgt gccaatttat atgctgcttc ctgggttgca
     6061 tcatcatcat aagccatttt gtatacatcc tgcgcattat aataacttcc ggcagacatc
     6121 aatgcatcgc catacatcat tttgcgggtg taattcattt tatctaattc cggattcgat
     6181 gtttgggcaa aacttgatcc tgccaacaga acgaatgtga gaattgcgaa tatttttttc
     6241 atatactatt tttctttatc agttgaaaat taattgaggt cacgtaatta ataacgagga
     6301 caaggcatta cataatgcgt tggaattacc ggtaaaatac aacctgtata taccaatgat
     6361 aattcgaaac caccgttacc tgaactagct tcttttaatg ttgaagtatt aatatcgtaa
     6421 ctggcaccga aacggaaatt tttaaaatcc atacctacag aagcgataat cgcatcatct
     6481 aaacgataag caccaccgag gaagaaggtt gccggaaaat ttgcatt
//
LOCUS       DAORKW010000162         6495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_411_length_6495_cov_5.095963, whole genome shotgun sequence.
ACCESSION   DAORKW010000162 DAORKW010000000
VERSION     DAORKW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_411_length_6495_cov_5.095963"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLB46_07810"
     CDS             <1..366
                     /locus_tag="PLB46_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF76461.1"
                     /translation="MLGHLTTFGGHPISCAAGLAAANFTITHNLVANSLAKAERIVSK
                     LQHKKIKQIRHSGLFFAIEFSSVEINMTINKKLIEAGLIVDWFLFAANCLRLAPPLTI
                     AEDEIDYACDMILSALKDE"
     gene            424..1401
                     /locus_tag="PLB46_07815"
     CDS             424..1401
                     /locus_tag="PLB46_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRF76462.1"
                     /translation="MKKLILTSFFAVSLVAYAAAPTPWGFWGHQRINNMALFTLPEDL
                     FGFYKPHIQYVTEHAVDPDKRRYAFKEEAPRHYIDIDHYGEPPYDAIPRKWNDAVIKY
                     SEDTLNEHGIVPWYIEKMMYRLTDAFKEKDVNYILKTSADLGHYVGDAHVPLHCTENY
                     NGQLTNQVGIHGLWESRIPELLGENFDYLTGKAEYIPDINAKVWEIIMESHALVDSVL
                     NLEAKLNAEFPTDQKYSYEARGASTVRVYSKAYTEKYDELMNDMVERRMRDAIIELGS
                     FWLTAWINAGKPNLTGIVIRPWTAEELKEMEDLDKSYKKGEFKGRDHTD"
     gene            complement(1474..4242)
                     /locus_tag="PLB46_07820"
     CDS             complement(1474..4242)
                     /locus_tag="PLB46_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF76463.1"
                     /translation="MKKYLFSVFLLVTAFSFQNAYGADCKSDFNFEIDGTTVYLIDDS
                     ESETGDISYFWTFGDGTTSTEASPTHTFTEPGIYEVCLTIETTGGCSDNRCELVTIGD
                     VISCTAGFEFFPDGLLCVFEDGSVSAPGDVIEFLWEFGDGETSTAENPEHTYSAEGTY
                     EVCLQIWTDDECTDVLCQPVVIGGVVGECEANFNFDLSGLTASFTDESILDAGEVIDW
                     SWTFGDGSTSELPNPSHTYDVAGVYNVCLLIITSEGCMADICYTIEVTGGADCFANFD
                     YEFDGATFFFASNTDPGPGDVDSYMWTFGDGAISDAENPTHTYAEPGVYNVCLVVHFA
                     TGCVAEFCNEIVVEGVGGDCTVNAEVVATDGLSKHFYAMVSPEVEEVTYTWFFGDGTT
                     FTETTAGTASDPWHTYTEAGVYNVCVVIETGAGCVDEYCFEIVVEAAGGGDCDADFEW
                     DADGLTVHFIETADGGGADIIFYFWEFGDGATSDAMNPFHTYDVEDHYEVCLTIITAE
                     GCISTFCDEINVEEAGGTCAASYVVTSTELTPDGWVVHFENTSTSAADITSTTWYFGD
                     ATIGETFDAEHLYTESGVYAVCLVISTADGCTDEYCFELFVGGGEGDCEADFEFDTEM
                     LTASFFENADGGGSDIVSYTWSFGDGATSDALNPEHTYDDSGSYLVCLTIVTADSCIS
                     TKCEEIDVEGEGGDCEAKFDVTSITETGSGWVIVLNNNSNGSEIYHWNFGDGGTAESE
                     NPDHLFELPGVYNICLQIGLEGGDCFDEKCEEVVIGGDECINETAIDSTHGCVDIYEP
                     VCGCDGITYTNSCHAIYYAGIVFYTDGACAATTIQEENIFGTVKLSPNPATYSTTLSY
                     ALKESGSVQIEITDITGNLALTPITHPSVPGNYTIQLNTQDLSSGIYIIRLTSNGNEV
                     QQKLMITK"
     gene            complement(4253..5716)
                     /locus_tag="PLB46_07825"
     CDS             complement(4253..5716)
                     /locus_tag="PLB46_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76464.1"
                     /translation="MEKEAVSLHWNYLKRLNMHLKNIPGKKLLVAALLSFAGVLTHQG
                     LIAQTVTEVANDTIRLCEGTTVVIDLLANDVDVDPGESLETDILVGAASVLIDYDDDA
                     LPEGSYSIEIDASFTGTDFMIYEVCGEDDLCATGILTIIVAGEEGCVWPGDANGDSIC
                     NYIDLLPIGVYYGLEGPVREDADGGWDEAFCDEWDEPIGIIYGNNPKFSDCNGDGLIN
                     AADTTVLVNNYGLLRGVYSPTEFIGGPEDPLISIDLLADTIAAGSKVVVPINFGTAGS
                     PATNIYGTTFDIVYDKTLIKKDSIKVTFNSGWLGTPGEDIIYLQKNDTLNGILSVSVT
                     RINQITRTGYDHFAELSFVMEDNIAGKTYDQITSTVNFCVELPQVINHKGVGIPVQTG
                     CDSVVALQFTGLQDQILNNQIITYPNPAHEAVNVILPENTTGQYLIINQLGEIIAQQQ
                     FTTNQFTISTQQIPSGNYILQIIADTGLIHKQLIIQH"
     gene            5929..>6495
                     /locus_tag="PLB46_07830"
     CDS             5929..>6495
                     /locus_tag="PLB46_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76465.1"
                     /translation="MLKHFSTKELSRFSDYLASPFFNKDEELLVFYNYVKKYAPEFDA
                     KNIEKERLLAKGIPGLTLNEKKIGYMMSDIVEHCENYIRYNNHFEEDIEGYVHLLSTY
                     NKWGTDKLFEQTLREARNTLTKNPFRNAPYFFKEYLLQSEVNLFFDRQKKRAYDASLQ
                     EAANFLDLFYISTKLKYSCELINRQKLVV"
BASE COUNT         2141 a         1302 c         1091 g         1961 t
ORIGIN      
        1 atgcttgggc atcttacaac ctttggtggc catcctatta gttgcgctgc cggtttagct
       61 gcggcgaatt ttaccattac acataatttg gttgcaaaca gtttggcaaa agcagaacga
      121 atagtttcaa aattgcaaca taaaaaaata aaacaaataa ggcattccgg tttgtttttt
      181 gcgatagaat tttcatccgt tgaaataaat atgacaatta acaaaaaatt aatcgaagcc
      241 ggtttaattg tggattggtt tttatttgcg gcaaattgct tgcgccttgc acccccatta
      301 acaattgcgg aggatgaaat cgactatgcc tgcgacatga ttttatctgc actaaaagac
      361 gaatagtgag tcctagtcca atgttttgaa ggatttttat tatccttgta ggaatattat
      421 cacatgaaaa agttgatatt aacttcattt tttgcggttt cacttgttgc ttatgctgcg
      481 gcgcccacgc cttggggttt ttggggccat cagcgtataa acaatatggc gctgttcaca
      541 ctgcctgaag atttattcgg attttataaa ccgcatattc aatatgtaac tgaacatgca
      601 gttgatcctg ataaaagacg ctacgctttt aaggaagaag caccaagaca ttatatcgat
      661 atcgaccatt atggcgaacc tccttatgat gcgattcctc gtaaatggaa tgatgccgta
      721 attaaatatt cggaggatac attaaatgaa cacggaattg ttccttggta tattgaaaaa
      781 atgatgtacc ggcttactga tgcatttaaa gaaaaagatg tgaattacat tttaaaaaca
      841 tcggctgatt taggtcatta cgttggtgat gcgcatgtcc ctttacattg cactgaaaat
      901 tataacggtc aattaactaa ccaagttggg attcatggtt tatgggagtc gagaattcct
      961 gaattgttgg gtgaaaattt cgattactta accggcaagg cagaatatat tccggatata
     1021 aatgcaaagg tgtgggaaat tattatggaa agccatgcct tagttgatag cgtgttaaat
     1081 ctggaagcaa aactgaatgc tgaatttcct acagaccaaa aatatagtta cgaagcaaga
     1141 ggggcatcaa ctgttcgtgt ttattcaaaa gcatataccg aaaagtatga tgaattaatg
     1201 aatgatatgg tggaaagaag aatgcgtgat gcaattattg aattgggcag tttttggtta
     1261 accgcatgga ttaatgcagg taaacctaat ttaactggta ttgtaataag accttggact
     1321 gctgaagagt taaaagaaat ggaagacctt gataagtcat acaaaaaagg tgaatttaaa
     1381 ggccgcgacc acacggatta aaatcttctt atttaactac aataaaaaaa gcgcacaatc
     1441 ataacaactg tgcgcttcta gtaataggag ctattatttt gtaatcatca gtttttgttg
     1501 cacttcattg ccatttgaag ttaaacgaat aatataaata cctgatgata aatcttgtgt
     1561 atttaattga atagtgtaat tacccggaac agatggatgt gttatcggtg taagtgctaa
     1621 attgccggta atatctgtaa tttcaatttg tactgagcca ctttctttta acgcataact
     1681 taatgttgtg ctataagttg caggattagg actcagtttc actgttccga aaatattttc
     1741 ttcctgaata gttgttgcag cacaagcgcc atcggtgtaa aatacgatac ctgcataata
     1801 aatggcgtga caagaattgg tataagttat tccatcgcaa ccacaaaccg gttcgtaaat
     1861 atctacacat ccgtgtgtgc tgtcaattgc agtttcgtta atacactcat ctccacctat
     1921 aacaacctct tcacattttt catcgaaaca atcaccacct tcgagaccta tttgtaaaca
     1981 gatattatat acacctggta attcgaataa atggtcagga ttttccgatt cagcagttcc
     2041 gccatcacca aaattccaat gataaatttc agaaccgttt gaattattat ttaaaacaat
     2101 tacccaacca gaaccggttt ctgttataga tgtaacatca aattttgctt cgcaatcacc
     2161 accttcacct tcaacatcaa tttcttcaca ttttgtgctg atgcaactat cggcagtcac
     2221 aatagttaaa catactaaat atgatcctga gtcgtcataa gtatgttccg gatttaaagc
     2281 atcacttgtt gcaccatcac caaaactcca tgtataagaa acaatatcag aaccgccacc
     2341 gtctgcattt tcgaaaaatg aagcggtaag catctctgta tcaaattcaa aatcagcttc
     2401 acaatctcct tcgccaccac caacaaataa ttcaaaacaa tattcatccg tacatccatc
     2461 ggctgtagaa attacaagac aaactgcata cactccactt tcggtatata aatgttctgc
     2521 atcaaatgtt tcaccaattg ttgcatcacc aaaataccaa gttgtagaag taatatcagc
     2581 agctgaagtt gaagtatttt caaagtgaac tacccatcca tcaggagtta attcagttga
     2641 agttactaca taacttgctg cacaagttcc acctgcttct tccacattaa tttcatcaca
     2701 gaatgtacta atacaacctt ctgcagtaat tatggtaaga caaacttcgt aatgatcttc
     2761 aacatcatag gtatgaaaag gattcatcgc atcagaagta gcgccatcac caaattccca
     2821 gaaataaaaa ataatatctg caccaccacc atcggcagtt tcaataaaat gaacagttaa
     2881 accatcggca tcccattcaa aatcagcatc acaatcgcca ccacccgcag cctcaactac
     2941 aatttcaaag caatattcat ctacacaacc tgcacctgtt tcaataacta cacaaacatt
     3001 ataaacgccg gcttctgtat atgtatgcca agggtccgaa gcagtacctg cagttgtttc
     3061 tgtaaatgtt gttccatctc caaaaaacca ggtataagta acttcctcta cttcagggga
     3121 aaccattgca taaaaatgtt ttgataatcc gtctgttgct actacttctg cattaacagt
     3181 gcaatcaccc ccaacccctt caacaacaat ttcattacaa aattctgcca cgcatccggt
     3241 tgcaaaatgt acaacaaggc aaacattgta aacgcctggt tcagcataag tatgtgtagg
     3301 attttctgcg tctgaaattg ccccatctcc aaacgtccac atataactat ctacatctcc
     3361 cggacctgga tcagtattgc ttgcaaaaaa gaaagttgcg ccatcaaatt cataatcaaa
     3421 gttggcaaaa caatcagcac cgccagtaac ctcaattgta taacaaatat cagccatgca
     3481 accttctgag gttataatta ataaacaaac attataaact cctgctacat cgtaggtatg
     3541 cgaaggatta ggtaattcag acgtagaacc gtcgccaaac gtccatgacc aatctattac
     3601 ttctccggca tccaaaatgg actcatcagt aaatgatgca gttagtcctg ataaatcaaa
     3661 attaaaatta gcttcacatt caccaaccac accaccaatg actaccggtt gacataacac
     3721 atctgtacat tcatcatccg tccaaatttg caaacaaact tcatatgttc cttcagcgct
     3781 ataagtgtgt tctggatttt cagcagttga agtttcacca tcgccaaatt cccataaaaa
     3841 ttctattaca tcccctggtg ctgaaactga accgtcctca aagacgcaca ataaaccatc
     3901 cgggaaaaac tcaaaacctg cggtacatga aataacatca cctatagtaa ctaattcaca
     3961 acggttgtca gaacaaccac cggtagtttc aattgttaga catacttcgt aaatgcctgg
     4021 ttcagtgaac gtgtgagttg gactagcttc agtagaggta gtgccatcac caaatgtcca
     4081 aaaataagat atatcccctg tttcagattc agaatcatct attagataaa cggtagtgcc
     4141 atcgatttca aaattgaaat ctgacttaca atctgcacca tacgcatttt ggaatgagaa
     4201 ggctgtaaca agcaaaaaga cggaaaataa atattttttc attgttgtgc gattaatgtt
     4261 gaataattaa ttgtttatgt attaaacctg tatcagcaat tatttgtaaa atataattac
     4321 ctgaagggat ttgttgagtt gaaatagtaa attgattagt tgtaaattgt tgttgtgcaa
     4381 ttatttcacc taactggtta ataattaaat attgacctgt tgtattttca ggcagtatta
     4441 catttacagc ttcatgcgca gggtttggat aagtaataat ttgattgttt aagatttgat
     4501 cttgtaatcc ggtaaattgt aatgcaacta ccgaatcaca tcctgtttgc acaggaatac
     4561 caaccccttt gtgattaata acctgcggta attcaacaca gaaatttacg gttgatgtaa
     4621 tttgatcgta tgttttaccg gcaatattat cttccattac gaagcttaat tctgcgaaat
     4681 ggtcgtaacc tgttctggta atttgattta tccttgtaac tgaaacacta agaatgccat
     4741 taagtgtgtc gtttttttgc agatatataa tatcctcacc cggtgtacca agccagccac
     4801 tgttgaacgt aacttttatg ctatcttttt taattaatgt tttatcatat acaatatcaa
     4861 atgtggttcc gtaaatattt gtcgccggag aacctgctgt tccgaaatta attggcacca
     4921 caaccttact tcccgcagca atggtatcag caagcaaatc gatgctaatt aatggatctt
     4981 cagggccacc aataaattcg gttggtgaat aaacaccgcg caataaacca taattattta
     5041 ccaatacggt cgtgtcggct gcattaatta atccatcacc attacaatct gaaaattttg
     5101 ggttattgcc ataaataata cctattggct catcccattc atcacaaaat gcctcatccc
     5161 atccaccatc cgcatcctca cggacagggc cttctaaacc ataataaaca ccaataggta
     5221 agaggtcaat gtaattacat atactgtctc cgttggcatc gcccggccaa acacagcctt
     5281 cttctcctgc tacaattatg gttaaaatac cagtggcaca caaatcatct tcaccacaaa
     5341 cttcataaat cataaaatcg gtaccagtaa atgaagcatc aatttcgatt gagtaggagc
     5401 cttccggtag tgcgtcgtca tcataatcaa ttaaaacact ggctgctcca accaaaatat
     5461 cagtttccaa cgattcaccc gggtctacat caacatcgtt tgctaacagg tcgataacca
     5521 cagtggtgcc ttcacacaaa cgaatggtat cattggcaac ctcggttaca gtttgcgcaa
     5581 ttaacccctg gtgtgttaaa acaccggcaa aactgagtag tgcagctacg agcaacttct
     5641 ttcctggtat atttttcaaa tgcatgttca gcctttttag ataattccaa tgtaaagaaa
     5701 cggcttcttt ttccatagaa aaaatacatt ttttcttatt ttccgtaatt tccaagctgt
     5761 tttttggtga ttttagtttc ttttttaact aaaaaatgat ataaaacatt gataatcagt
     5821 actatataat attgacaagt tttaatattt tgctcccaaa tacctgattt tccgaaaaat
     5881 aatggtattt ttcggactgt ttatatgtat aaaagcaaga tgatagaaat gctgaagcat
     5941 ttcagcacaa aagaactgag caggttttct gattacctgg cttcaccctt ttttaataag
     6001 gatgaagaat tgctggtgtt ttataattat gtaaaaaaat atgctcccga gtttgatgca
     6061 aaaaacattg agaaggaacg actgttagcc aaaggaatac cgggacttac gctcaatgaa
     6121 aaaaagattg gctatatgat gagcgatatt gttgaacact gcgaaaatta tatccgttat
     6181 aataatcact ttgaggaaga tattgaaggt tatgtccatt tactgagcac ctataataaa
     6241 tggggaaccg ataaattatt tgaacaaaca cttcgcgaag caaggaatac attaacaaaa
     6301 aatccattca gaaatgcgcc gtattttttt aaagaatatt tattacaaag tgaggtaaat
     6361 ttatttttcg accgacaaaa aaaacgtgct tacgatgcca gtctgcagga agctgctaat
     6421 tttttagatt tattttatat ctccaccaaa ctcaaataca gttgtgaact catcaaccgg
     6481 caaaagttgg ttgtt
//
LOCUS       DAORKW010000163         6495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_412_length_6495_cov_4.640373, whole genome shotgun sequence.
ACCESSION   DAORKW010000163 DAORKW010000000
VERSION     DAORKW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_412_length_6495_cov_4.640373"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PLB46_07835"
     CDS             <1..706
                     /locus_tag="PLB46_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRF76466.1"
                     /translation="TTSKIAFTDGNTKFSEAGVQFIINPYDEWYALVRALELKETLGG
                     TVTVINAGKADSEQIIRKALALGADDAVRIDLDPADAYQVAANIAAYAKNEQFDIILT
                     GKETIDYNGGVVGGMLAEMLDLPYVSMSSKLDVDGSILKIEREMEGVKETVQVKTPCV
                     ISAQKGMAEARIPNMRGILASKTKPVKVINPDVTIVKAGVKVFALPPEKTGCKMISPD
                     DAAQLVGMLHNDAKVI"
     gene            741..1694
                     /locus_tag="PLB46_07840"
     CDS             741..1694
                     /locus_tag="PLB46_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRF76467.1"
                     /translation="MTNLVFAELSNGKLRKTSHEVVAYSCKLGATTAVAIGQADAGEL
                     EKLGAYGVTKVLHVNDQRLNNVFSKAYTKILVETAAMVGADTILLSHNSAGKSIAPRV
                     AVRWNASLYPGVNSLPENGSIRKAVFSGKAFADLTLGEGLKVITLNPNSYKPEVTGGT
                     ATIEALSANITDADFDYTVLSIEDESGGHIPLPEAEIVVSGGRGLKGPENWGIIEDLA
                     NALGAATACSRPVADIGWRPHHEHVGQTGITIKPTLYIAVGISGAIQHLAGVNQSKVI
                     VVINKDPEAPFFKAADYGIVGDAFEILPKITEAVKKLKSEG"
     gene            1773..2399
                     /locus_tag="PLB46_07845"
     CDS             1773..2399
                     /locus_tag="PLB46_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HRF76468.1"
                     /translation="MQKIELEISGLSHTITQSQSYAVLLSEVGGMRKLPIVIGAFEAQ
                     AIAVAIEKVVINRPLSHDLIKNLFTIFNISLKEVLIDNLQDGIFYSKLICEKDGQEIE
                     VDSRTSDALALAVRFNCPIYTFEFILETAGAIINEQDKKEDDEDDDDEEVDEDTAEIV
                     GAALSADADDIALMSLKELNDKLKEYLDKEDYESAARIRDEIEQRKNK"
     gene            2411..2782
                     /gene="crcB"
                     /locus_tag="PLB46_07850"
     CDS             2411..2782
                     /gene="crcB"
                     /locus_tag="PLB46_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRF76469.1"
                     /translation="MQIVLLIGLGGFFGTIVRYLIGNVFAHYNKPQFPFATLTVNLLG
                     CFIIGLIYGWFEKQQLDSTDWKLILTAGFCGGFTTFSAFSMENIQMIRDGQTLMAILY
                     IFSSLLFGLLLTWLGMSLSRT"
     gene            2832..>3939
                     /locus_tag="PLB46_07855"
     CDS             2832..>3939
                     /locus_tag="PLB46_07855"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HRF76470.1"
                     /translation="MIENILRGLLGVAFLVGICWLFSKNRKAIDWKLVGTGLLIQFTF
                     AFLIIALPNITENYFGYRFDWFERIFRGMANFFVMVIGYTNAGATFVLGDWPSVTQVN
                     DGLTGELFTVGYIFAFKVLPTVIFFSALTSLLYFLGILQKIVFGFAWVMSKTMRLSGA
                     ESLNAAGNIFLGQTEAPLLIRPYLDKMTKSEILCVMVGGMATIAGGVMAAYIGFLGGD
                     DVERQKLFAMHLLSASIMSAPAAIVASKMLFPQTESVDRDLKVNKERIGSNVIEAIAN
                     GTTDGLKLAVNVGAMLLAFTAFMAMFNSIFLGLGNVIGINDNIASATNNTYSGLSLDY
                     LFGIIMAPVAWLLGTPSGDILAVGQLLGEKTIINE"
     assembly_gap    3940..4039
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4040..4333
                     /locus_tag="PLB46_07860"
     CDS             <4040..4333
                     /locus_tag="PLB46_07860"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HRF76471.1"
                     /translation="GDILAVGQLLGEKTIINEFVAYASLGEMKDSLDEKSIIIATYAL
                     CGFSNFASIGIQIGGIGALAPNQKTNLSQLGIRALLGGTVACFFTAAIAGMII"
     gene            4343..4600
                     /locus_tag="PLB46_07865"
     CDS             4343..4600
                     /locus_tag="PLB46_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76472.1"
                     /translation="MGEFYQWVIFLGFLAIIIIVIYLFAGGYIRTQRTQTESFKEWQK
                     FNGGVVRMMCILLLLVCIGIGVYKYYQVNNSITAPPSLETD"
     gene            complement(4597..5547)
                     /locus_tag="PLB46_07870"
     CDS             complement(4597..5547)
                     /locus_tag="PLB46_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76473.1"
                     /translation="MRKLLSAYILFLVTVIQLTAQNCNGNATGNIPIPDLGAQQFNGF
                     TGGKYLQGNNQIPASHYTMGLLSHEKIQPLNTFGETDSVSGKIGMMVLGFSTAAMTGR
                     FVRTFLELKQENTYLDFFIGAQGGRDINSMTNLQSTYWTNIDSTLEAETLSRLQVQII
                     WLSSGDIYTFNEPFPQQCYTQIEKYRLTLQNIKTIYPNCQVVYLSDRTYAGYIGQNGE
                     GPAELKEPTAYYNGWTIKWLIENQITNKAGFTYTDIPFIDWGPYLWTDGINGNNQGYT
                     WECDDAGKGGIHPSSKGRMKEAAMVYLYFSQHPLLRKIWY"
     gene            complement(5549..>6495)
                     /locus_tag="PLB46_07875"
     CDS             complement(5549..>6495)
                     /locus_tag="PLB46_07875"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HRF76474.1"
                     /translation="PLYLANIAISGGTGVQQFNTQGISPLVNSLYSLLLEENYLKLYE
                     QQYFNIKGGRELTNGFYVEGQMHYTQRSQLFNLATPDIWYDRENNGYSSNNFPFYTDS
                     LQFKIPTKSSVKISGRYAFGQTYIMLPDEKNIINSKYPTINFSWEKAIPGVFGSSVSY
                     DILRFGVEDKIKLALAGNIMINTEVGKMFAHNNLNEADLVHFLANETIIERIADEAYF
                     LLPYYLASTDNSFVSGHFQWHTEGFLFRQLPLFKQLKMEPVFSCNYLYTENIGHYVEL
                     AAGVEHLFKIIRIDAAFTPYQFTNYPSEKFKLLIGFGF"
BASE COUNT         2034 a         1047 c         1273 g         2041 t
ORIGIN      
        1 tactacttca aaaattgcct tcaccgatgg caatactaaa tttagtgaag ccggtgtgca
       61 gtttattatt aatccttacg atgaatggta tgcgcttgtt cgcgcacttg aactgaaaga
      121 aacgcttggt ggtactgtta ccgtaattaa tgccggcaaa gcggacagtg aacaaattat
      181 acgtaaagca cttgctttgg gagctgatga cgccgttaga atagatttag accctgcaga
      241 tgcttaccaa gtagcagcta atatagcagc ttatgctaaa aatgaacaat tcgatatcat
      301 tctaaccgga aaagaaacca ttgattacaa tgggggtgta gtaggaggca tgttggcaga
      361 aatgcttgat ttaccatatg tttccatgag tagcaaattg gatgttgatg gcagtatctt
      421 aaaaattgaa cgtgaaatgg aaggggtaaa agaaactgtt caggtgaaaa ccccatgtgt
      481 aattagtgct caaaaaggta tggctgaagc gcgtattcca aatatgcgtg gaattttagc
      541 ctctaaaact aaaccggtaa aagtgattaa tccggatgtg acaatagtga aagcaggcgt
      601 taaagtattt gctttaccac ctgaaaaaac cggttgtaaa atgattagcc ctgatgatgc
      661 cgctcaattg gtgggtatgt tgcacaacga tgctaaagta atttaattca attcaaaaaa
      721 agttatttaa atatacggat atgacgaatc tggtttttgc agagttatcg aatggtaaat
      781 taagaaaaac ttcacatgaa gttgtagctt attcatgtaa attaggcgct actacagctg
      841 ttgcaattgg tcaggctgat gcaggtgagt tagagaaatt aggcgcttac ggtgtaacaa
      901 aagtgttaca tgttaacgac cagcgtttaa ataatgtatt ttcaaaagca tacacgaaaa
      961 tattggttga aacagcagca atggttggtg ctgatactat tttgttatcg cataattctg
     1021 ccggaaaaag tattgcgcca agagtagctg tgagatggaa tgcatcgctg tacccaggcg
     1081 taaatagttt accggaaaat ggcagtataa gaaaggctgt attttcaggt aaggcttttg
     1141 cagatttgac attaggtgaa ggcttaaaag taattacact caatccgaat tcatataaac
     1201 ctgaagtaac aggcggcact gcaactattg aagccttatc agcaaatatc actgatgctg
     1261 actttgatta tactgtgtta agtattgaag acgaatccgg tggtcatatt ccattaccgg
     1321 aagctgaaat tgtagtttca ggtggaagag gcttaaaagg acctgaaaat tggggtataa
     1381 ttgaagattt ggcaaatgca ttaggcgctg caactgcgtg ttcccgccct gttgcagata
     1441 taggttggag acctcatcat gagcatgtgg gacaaaccgg catcactatt aaacctacgt
     1501 tatacattgc agtggggatt tcgggtgcta ttcagcatct ggctggtgtt aaccaaagta
     1561 aagtgattgt tgtaatcaac aaagacccgg aagctccatt ttttaaagct gctgattacg
     1621 gcattgttgg tgatgcgttt gagattttac caaaaataac agaggctgta aaaaagttaa
     1681 aatctgaagg ctaagcattt ttaaattaat cttacatttc agataaacat cctaaaataa
     1741 ctttttaact tagtaactct aagcagaccg caatgcaaaa aattgaactt gaaatttcag
     1801 ggctttccca taccataaca caatcacaaa gttatgccgt attgttaagc gaagtaggag
     1861 gcatgcgcaa gctccccatt gtaataggtg catttgaagc gcaggcaatt gctgttgcaa
     1921 ttgaaaaagt agttatcaat cgaccattat cccacgattt aattaaaaac ctgtttacca
     1981 tttttaatat ctcgttgaaa gaagtactta tcgacaattt acaggatggt attttttact
     2041 caaaattgat atgcgaaaaa gacgggcagg aaattgaagt tgatagcaga accagcgatg
     2101 cccttgcatt ggctgttcgt tttaattgtc ccatttatac tttcgagttt attctggaaa
     2161 ctgccggagc aattattaat gaacaggata aaaaagaaga tgatgaggat gatgatgacg
     2221 aagaagttga tgaggataca gcagaaatag taggtgccgc actatctgcc gatgctgatg
     2281 atatagccct aatgagtttg aaagaattaa atgacaaatt aaaagaatac ctcgataaag
     2341 aagattatga atccgctgct agaataaggg atgaaataga acagcgcaaa aacaaataaa
     2401 tgccttattt atgcaaattg tactactcat aggcttagga ggattttttg gtacaattgt
     2461 ccgatatctt atagggaatg ttttcgcaca ttacaataaa ccacagttcc cgtttgcaac
     2521 cttaactgtt aacctgcttg gatgtttcat tattggttta atttatggct ggtttgagaa
     2581 gcaacaattg gatagtacag attggaaatt gatacttacc gctggctttt gtggcggttt
     2641 tactacattt tcggcatttt caatggaaaa catacaaatg atacgtgatg gccaaacctt
     2701 aatggcgatt ttatatatct ttagcagcct tttgttcgga ttattactta cctggctggg
     2761 tatgtcgcta agcagaactt aagtccgctg attttatatt tgattataat tgattaccaa
     2821 aaattcacac aatgattgaa aatatattaa gagggttact tggtgttgct tttttagttg
     2881 gtatttgttg gttgtttagt aaaaaccgta aagctattga ctggaaattg gtgggcaccg
     2941 gattactcat tcaatttaca tttgcttttt taatcattgc tttacctaat atcaccgaaa
     3001 attattttgg ctaccgcttc gactggttcg aaagaatttt taggggcatg gcgaatttct
     3061 ttgtgatggt tattggatat actaacgcag gcgccacatt tgtattagga gactggcctt
     3121 ctgtaactca ggtgaatgat ggattaaccg gagaattatt taccgtagga tatatttttg
     3181 catttaaggt attacccact gttattttct tttccgcctt aacctcttta ttatattttt
     3241 tagggatact tcaaaaaata gtatttggat ttgcttgggt tatgagtaaa accatgcgct
     3301 taagcggtgc cgagagttta aatgctgcag gcaatatttt tcttggacaa acagaggctc
     3361 cattattaat cagaccttat ttagataaaa tgaccaaatc ggaaatttta tgtgtaatgg
     3421 ttggagggat ggcaactatt gccggaggtg ttatggcggc atatattgga tttttaggag
     3481 gtgatgatgt ggaacgccaa aaattatttg cgatgcattt attaagtgct tcaattatga
     3541 gcgcacctgc tgccattgtt gcatcaaaaa tgttatttcc gcaaacagaa tcggtagaca
     3601 gagatttaaa agtgaataaa gaacgtattg gttcaaatgt aattgaagca attgccaacg
     3661 gtactactga tggcttgaaa ttagccgtta atgttggtgc gatgttatta gcatttacgg
     3721 ctttcatggc gatgtttaat tcgatattct tgggtttagg aaatgtaatt ggtattaacg
     3781 ataatatagc aagcgccact aataatacct attccggttt atcacttgat tatttatttg
     3841 gaattattat ggctcccgtt gcctggttgc tgggaacacc gtccggagat attttagctg
     3901 ttgggcaatt acttggtgaa aaaacaatta tcaacgaatn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnng gagatatttt agctgttggg caattacttg gtgaaaaaac
     4081 aattatcaac gaatttgttg cgtatgccag cttaggagaa atgaaagatt cattggatga
     4141 aaaatcaata attattgcta catatgcatt atgcggcttt tccaattttg cttcaatagg
     4201 cattcaaatt ggtggtatag gggcattggc accaaatcaa aaaacaaatt tatcgcaatt
     4261 gggtattcgt gcattattag gtggaactgt cgcctgcttt tttacagcag caattgcagg
     4321 aatgattata taagtacata ctatgggaga attttatcaa tgggttattt ttcttggatt
     4381 tcttgcaatc attataatag ttatttattt atttgcagga gggtatatcc gtacacaacg
     4441 cacacaaacg gagagtttta aagaatggca aaaattcaat ggcggtgtag tgcgaatgat
     4501 gtgtatatta ctcttactgg tttgtatagg aattggtgtt tataaatatt atcaggtgaa
     4561 taacagtatt actgctccac catcattaga gactgattaa taccaaattt ttcgcaataa
     4621 aggatgttgt gaaaaatata aataaaccat ggcagcttct ttcattctgc ctttggagga
     4681 gggatgaatg ccacccttac cggcatcatc acattcccat gtataaccct gattattacc
     4741 attaattcca tcagtccata aatagggccc ccaatcaatg aaaggaatat cagtatatgt
     4801 gaacccggct ttattggtaa tttgattttc aatcaaccat tttattgtcc atccattgta
     4861 ataagcggtt ggttctttta attccgctgg accttcgcca ttttggccaa tatatcctgc
     4921 ataagtgcga tcagataaat acacaacctg gcaattggga tatattgttt ttatgttttg
     4981 taaagtcaac cggtattttt ctatttgagt ataacattgt tgaggaaatg gctcattaaa
     5041 cgtataaatg tcaccggaac ttaaccagat tatttgcact tgcaaacgag acaaggtttc
     5101 tgcctccaag gttgaatcga tattggtcca ataggtactt tgtaaattgg tcatagaatt
     5161 aatatcacga cctccttgtg caccgataaa aaaatcgaga taagtatttt cctgttttaa
     5221 ttccaaaaat gtgcgcacaa aacgccctgt cattgcagca gtactaaatc ctaataccat
     5281 cattccaatt ttgccggata cactatcggt ttcaccaaat gtatttaatg gttgaatttt
     5341 ttcgtgactc aacaatccca tagtataatg cgaagcggga atttgattgt ttccctgaag
     5401 atattttcca cctgtgaagc cattaaattg ctgcgcgcct aagtcaggaa taggaatatt
     5461 tcctgttgca ttaccgttac agttttgtgc agtcaattga ataactgtaa ccaaaaaaag
     5521 tatataagct gataacaatt ttcgcatatt aaaatccaaa gccaattaat aatttaaatt
     5581 tttcggaagg ataatttgta aattgatagg gcgtaaaagc ggcatcaatt ctaattattt
     5641 taaaaagatg ttcaacgcct gctgccaatt caacataatg cccaatattt tcagtatata
     5701 aataattaca tgaaaaaacc ggttccattt ttaattgttt gaacaagggg agttgtctga
     5761 ataaaaatcc ctcagtatgc cattgaaaat gaccactcac aaaactatta tctgttgatg
     5821 ctaaataata aggtaataaa aagtaagctt catcagctat acgttcaatg atagtttcat
     5881 ttgcaagaaa atggacaaga tcagcttcat ttaggttgtt gtgggcaaac attttaccaa
     5941 cttctgtatt tatcattata ttacctgcca atgctagttt aattttatcc tctacaccaa
     6001 atcttaaaat atcataactt actgaacttc caaaaacacc gggtattgct ttctcccatg
     6061 aaaaattaat ggttggatac ttactattga taatattttt ttcatccgga agcatgatat
     6121 aagtttgtcc aaaagcatat ctgcctgaaa ttttaacact actttttgtt gggattttaa
     6181 attgaagtga atcagtataa aatggaaaat tgttggaact gtagccattg ttttccctgt
     6241 cgtaccaaat atcaggagtt gctaaattaa aaagttgtga gcgttgcgta tagtgcattt
     6301 gaccttctac ataaaaacca tttgttaatt ctcgaccacc cttaatattg aaatattgtt
     6361 gctcataaag tttcaaataa ttttcctcca atagcaaact atacaatgaa ttaaccaatg
     6421 gtgaaatacc ctgagtatta aattgttgca caccggttcc acctgaaatt gcgatgttcg
     6481 cgaggtacaa cggat
//
LOCUS       DAORKW010000164         6471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_413_length_6471_cov_4.259975, whole genome shotgun sequence.
ACCESSION   DAORKW010000164 DAORKW010000000
VERSION     DAORKW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_413_length_6471_cov_4.259975"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLB46_07880"
     CDS             complement(<1..230)
                     /locus_tag="PLB46_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76475.1"
                     /translation="MAIVMGLTQCKKDTFEIDYSNFNKVIKKGAFEIYIPKYLHLDKK
                     LQPKAAVAYSDSLNNSFYIIIREEIPDTEDDS"
     gene            complement(251..1114)
                     /locus_tag="PLB46_07885"
     CDS             complement(251..1114)
                     /locus_tag="PLB46_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76476.1"
                     /translation="MNVKLPLFLCFLYSATAFSQSPTTVNGLFVYQDSLKIPLTYEIG
                     PGQLTKNGSHFILGLMDGELDEYVNLVSDIYSIDVSATNPASAIKGFQLPNAPDSVRY
                     FQCSASANEEVLVYVSNLYAGWNDNELAISYKQPDGTYGKHRMLKELNDSLESDAYPW
                     ISADGLRIYYNRNFKMLYAERKSWDEPFSKPAPVTFQGQVNLEIVSAWFTDDELNMFI
                     VANNVIYRASRKSINEPFSFPEVFTNEFKNVYFIAGLSFMPDKKTLFLYYSTEDKQSI
                     LKYQLKKGKAW"
     gene            1243..1782
                     /locus_tag="PLB46_07890"
     CDS             1243..1782
                     /locus_tag="PLB46_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76477.1"
                     /translation="MLLALIDEFEKRKLQLIGVDKLRAMVTKRLDDERIAAEAEAIVI
                     EPELPLPTLFSQTTILAFTIFFSPLFGGALLAMNVAAVKKTGVWQVLSLSLALTVLVG
                     VLNMYLIPPGSIFGLLILAAIALFMSELLWNRFIGKKVSYKNRSFTVPLIIALAISIP
                     LIYYFYQHPELLELTTNTK"
     gene            1779..2393
                     /locus_tag="PLB46_07895"
     CDS             1779..2393
                     /locus_tag="PLB46_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76478.1"
                     /translation="MKIKITGLVLGLLIMVNTLKADSPLTSTYFAASYYDYSIVSQAE
                     VNRGLNDEIAAYLLNEDNPIDVKAAIINGIGWNYDGTNNSELFKEYLAKSKGITTAQL
                     NLDDLSAHQLFCLGYLMGLDNYFVVDEAINVLEKAAAKNKTSFTIHLVLNITKAQKAM
                     DTDFCKVWKLTAETLNNKTLKRDMKTAAIQSVVDYMLLYKGDCI"
     gene            complement(2400..4694)
                     /locus_tag="PLB46_07900"
     CDS             complement(2400..4694)
                     /locus_tag="PLB46_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76479.1"
                     /translation="MRYWYIYMSFLLLSCGASSVEQANIPNTWPNKQTLMEQFRQADT
                     AFIIYAKEDYAIADIVINGIAAAKMRDDMFAIIPVRDDEVTEQQISQHPIYVIGTRSN
                     LVLNRINDHLPIRFFENGFEFDGKKYTTRDAIYRINFYPNLYNPKFPLTVISGNNEDE
                     LVKFLQEQFKESWGYFYWDNWGYQIYTNGQRSVLGNFSEDSASLWTIDKKLHWDFDFK
                     GEIVKETALINYYNHSVEDVKVLTDDLDKHNQQNLAALEQFTGKKLQHGIKYHLYKSS
                     ELKALMMGNADQSMIDFEEYAIHSVMTNEFKGKYDETFSMLVVRNLLGQPAIYALEEG
                     LAVKFTQQWQGTSYTYLAGNLAKADALPTLAALISNDAFEGESDYLMHIASASLVDFL
                     IDKYGAQQFLANYLKWQQNSPELIALQKEWYAFVINAANNNQLNSILKDTPKQSFYKG
                     FNYAHEGYQIYNGYMGSTSDQSIDKLKELGVNSLTIIPYSGFRSMNKPFPISYTTGAG
                     GENDASILHAAYTAHQNGMSVMLKPQLWSWLGWTGDITMTNQKDWDLFFEYYEQWIMH
                     YALMAEMYRFEMFCIGVEFQNASLSEHNKWDELFDKVRKIYTGKITYAANWGKEFETV
                     SFWDKLDFIAVNCYYPLSTKMNPTDEELLTAFEKNLDVIETVSKKYQLPVVFTEIGFK
                     SIAAPWIQPHKDADEQDYNEHSQQRCYEVMIKAMADEPWIDGVYLWKWPSYMDFSTEY
                     NKDFTPCGKLAEKTISNWFLNSNN"
     gene            complement(4770..5519)
                     /locus_tag="PLB46_07905"
     CDS             complement(4770..5519)
                     /locus_tag="PLB46_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF76480.1"
                     /translation="MINIAIIEDEKIAADYLEKLILSFPADITVMAKLDDVTSAVAFL
                     SKNKPDLLFCDIHLGDDISFSIFNKIEVTAPVIFTTAFDQYAIKAFKVNSIDYLLKPI
                     NKDELFAAINKFQQSTGNPTKNIDLLLNHFTQTQNYQQRFLVSSGSKLKSVSIADVAY
                     FFADQKLNFLVEKTGKQYIVDSSLDKLEPTLNPTQFFRINRQFIISFDSIKSMTNYSK
                     SRIKIELNPPIERETIVSVERSPDFKHWLNR"
     gene            complement(5516..>6471)
                     /locus_tag="PLB46_07910"
     CDS             complement(5516..>6471)
                     /locus_tag="PLB46_07910"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF76481.1"
                     /translation="PCLTARDYAISCIFSVLVTFSLFLANIYIIKTITTKYEEKLSAF
                     KRISLSLGLGLIISNIIIYFEWQIFNHLFYKLTDQEAKMSVFQNQMFATVLVSIVSLV
                     LEIRHYVEKLKISIAEKEKLEREFLRAQLEGLKTQISPHFLFNSFNALQSLIEDDTTK
                     AKEFVQELARVYRYVLEKRDQLVVTLHDEIQFIQSFIYLNKIRFGDNLIVETQIDADS
                     LNHYLPPLTLQLLVENAIKHNVISNAKPLVIQIIAKGDKLTVSNIFQPRAEKLQSTQI
                     GHQNLISRYQLICDSIPKFGLIDNQYVAQIPLVSQTVAEEL"
BASE COUNT         2038 a         1101 c         1114 g         2218 t
ORIGIN      
        1 atactgtcat cttcggtatc gggaatttct tctctgatga tgatataaaa tgagttattc
       61 aggctatcgg aataagcaac agcagctttt ggttgtaatt ttttatctaa gtgcagatat
      121 ttggggatat aaatctcaaa cgctcccttc ttaattacct tattgaagtt actataatca
      181 atttcaaacg tatctttttt gcattgagtt aaccccatta caatcgccaa aatcagatat
      241 gcaacttttt ttaccaggct ttaccttttt tgagttggta ttttaaaatg ctttgtttgt
      301 cctcagtaga atagtataaa aataatgttt ttttatccgg cataaaactc aatccggcaa
      361 taaagtaaac gtttttgaat tcattggtaa atacttcagg aaacgaaaat ggttcattaa
      421 tcgatttacg ggaagcacga taaattacat tattggcaac aataaacata ttcaattcat
      481 catccgtaaa ccaggcgcta actatttcca gatttacctg accttgaaac gttacaggtg
      541 ccggtttaga aaatggttca tcccaacttt ttctttcggc atataacatc ttaaaattcc
      601 tattgtaata aatgcgcaaa ccatcagctg atatccatgg gtaagcatca ctttccagtg
      661 aatcgtttag ttcttttaac attctatgtt tgccatatgt gccgtccggt tgtttgtagg
      721 aaatggctag ttcgttgtca ttccatccgg catataagtt ggaaacatac accaacactt
      781 cttcgtttgc cgaagctgaa cattgaaaat aacgcactga atcaggtgca tttgggagct
      841 gaaaaccttt tatggctgaa gctggattag ttgcggaaac gtcgatggaa tagatatctg
      901 aaaccagatt aacatattca tccaattctc catccattaa ccctaaaata aaatggctac
      961 catttttcgt taattgtccg gggcctattt cataggttag cgggattttt agggagtctt
     1021 ggtagacaaa aagcccgtta acagtggttg gcgactgtga aaatgcggtt gcagagtaca
     1081 aaaagcagag aaatagcggt agttttacgt tcatattcaa ttattataca aaaatataaa
     1141 tttatcgata tgcaggataa tacctacaaa atggttgtag caaggttagc tgatgaggac
     1201 atattggagg cattaagaaa tcatgaaaag tatgtcgatg gtatgttatt ggcattaatt
     1261 gatgagtttg aaaagcgtaa attacagtta ataggggttg acaagttacg tgccatggta
     1321 acaaagcgcc tggacgatga aagaattgcc gccgaagccg aagctattgt tatagaaccg
     1381 gaattgcctt taccaacgct tttttctcaa acaacgattt tagctttcac tatatttttc
     1441 tcgccattat ttggtggtgc tttgctggct atgaatgtag ccgcggtgaa aaaaaccggt
     1501 gtatggcagg ttttatcttt atcattagcg ctaactgtac tggtgggagt gttaaatatg
     1561 tatttaatac ctccaggtag catttttgga ttattaatat tagctgccat agcattattc
     1621 atgtctgaac ttttatggaa ccgatttatc ggtaaaaaag taagttataa aaacagaagt
     1681 tttacagtgc cattaattat tgcattggca atttccattc cattaattta ttacttctat
     1741 caacacccgg aattacttga attaacaacc aatacaaaat gaaaattaag ataacaggat
     1801 tagttttagg cttattaata atggtgaata cactaaaagc tgattcacca ctaacttcca
     1861 catattttgc tgccagttat tacgattatt ccattgtatc tcaagcagaa gttaatcgtg
     1921 ggttgaatga tgaaattgca gcatacttat taaacgaaga taatcctatt gatgttaaag
     1981 cggcaataat caatggtatc ggttggaact acgatggtac taataacagt gaattattta
     2041 aagaatattt ggcgaagtcg aaaggtatta ccacagctca attgaactta gatgatttat
     2101 ctgcgcatca attgttttgt ttagggtatc tgatgggttt ggataattat tttgttgtag
     2161 atgaagctat caatgttttg gaaaaggctg ctgcaaaaaa taagacaagt ttcaccattc
     2221 atcttgtatt aaatattacc aaagcgcaaa aagcaatgga tacagacttt tgtaaagtct
     2281 ggaaattaac tgcagaaaca ttaaataaca aaacactcaa acgggacatg aaaacagctg
     2341 ctattcagtc tgtagttgat tacatgttgt tatacaaagg cgattgtatc taaatcgttt
     2401 taattattgc tatttaaaaa ccaattgctg attgtttttt cagccaattt gccacatggt
     2461 gtaaagtcct tattgtattc ggtgctgaaa tccatataac ttggccattt ccataagtaa
     2521 acaccatcta tccacggttc atctgccatt gctttaatca taacttcgta acaacgttgc
     2581 tgactgtgtt cattgtaatc ctgttcatct gcatctttat gtggttgtat ccatggtgcg
     2641 gcaatacttt taaatcctat ttcagtaaat acaacaggca attgatattt tttactaacg
     2701 gtttcaatta catcgaggtt tttttcaaat gcagttaata attcttcatc tgtaggattc
     2761 atttttgtgc tcaaagggta ataacaattt actgcaataa aatccagttt atcccaaaac
     2821 gaaacggttt caaattcttt cccccagttg gctgcatagg tgattttacc ggtatatatt
     2881 ttccgcacct tgtcaaaaag ttcatcccat ttgttatgtt ccgaaagtga tgcgttttga
     2941 aattcaacac cgatacaaaa catttcaaag cgatacattt cggccattaa ggcataatgc
     3001 ataatccatt gttcataata ttcgaaaaat aaatcccagt ccttttggtt agtcatcgta
     3061 atatcacctg tccatcccaa ccaagaccat aattgtggtt ttaacattac cgacatacca
     3121 ttttggtgtg ccgtataagc cgcatgtaat atactcgcat cattttcacc tcctgcacct
     3181 gtagtatagg atattggaaa aggtttattc atagaacgga atccactgta gggaataatt
     3241 gttaatgaat ttacacccaa ctctttcaat ttgtcaattg attggtcgga agtagatccc
     3301 atgtaaccat tataaatttg atacccttca tgtgcatagt taaagccttt ataaaatgat
     3361 tgttttggtg tatcctttaa tattgaattc aattgattat tgtttgcagc atttattaca
     3421 aacgcatacc attctttttg taatgcaatt aattcgggac tgttttgttg ccattttaaa
     3481 taattggcta aaaactgttg agcaccatat ttatcaatca gaaaatcgac aagacttgca
     3541 gaagcaatat gcatcaaata atctgattcg ccctcaaatg catcgttaga aattagtgca
     3601 gctaaggtgg gtaaagcatc tgctttagcc aggttacctg ctaaataagt gtaagaagtg
     3661 ccttgccatt gttgagtaaa tttaaccgct aatccttctt ctaaagcata tatggcaggc
     3721 tgtcccaaca agttgcggac aactagcatg gaaaatgttt catcgtattt gcctttaaat
     3781 tcattggtca taacactatg tattgcatac tcctcaaagt caatcatact ttggtctgca
     3841 ttgcccatca taagcgcttt taattccgat gatttataca aatgatattt aataccgtgt
     3901 tgtagttttt taccggtaaa ctgctccaga gcggctaaat tttgttgatt gtgtttatct
     3961 aaatcatccg ttaacacttt aacatcctca acactgtgat tataataatt tattaacgca
     4021 gtttctttta ctatttcgcc tttaaaatca aaatcccaat gtaatttttt atcgatagtc
     4081 cataatgaag ctgaatcttc tgaaaaattg cccaatacac tgcgctgccc attggtataa
     4141 atctgataac cccagttatc ccaataaaaa tatccccaag actctttaaa ttgttcttgt
     4201 aaaaatttta ctaattcgtc ttcattattg cctgaaataa ctgttaatgg aaatttaggg
     4261 ttgtataaat tgggataaaa attaattcgg tagatagcat ctctggtagt atatttttta
     4321 ccatcaaact caaaaccatt ttcaaaaaat cggataggta aatggtcgtt aattcggttc
     4381 agaaccaaat tgcttctggt gccaattaca taaataggat gttgtgaaat ctgctgttca
     4441 gtaacttcat catcgcgaac cggaattatg gcaaacatat catcacgcat tttggctgcg
     4501 gcaataccat taataacaat atctgcaatg gcatagtctt ccttcgcata aataataaag
     4561 gctgtatccg cctgccggaa ttgctccatg agtgtttgtt tgttcggcca tgtattagga
     4621 atatttgctt gttcaacgct agatgctccg caagacagca ataggaacga catgtagata
     4681 taccaataac gcataagttt gcaaaattat cggaaaattg ggaattatac tcgttgggcg
     4741 aggctgttaa gcaattttaa ctgtagaatt tatctgttta gccaatgctt aaagtccgga
     4801 ctccgctcaa cacttacaat ggtttcacgt tctattggcg ggtttaattc aatttttatc
     4861 ctggatttcg aatagttggt catactctta atactatcga aactaataat aaactgtctg
     4921 tttatacgaa aaaattgtgt tggatttaat gttggttcca atttatccag tgatgaatca
     4981 acgatatatt gtttccccgt tttttctact agaaaattga gtttttggtc tgcgaaaaaa
     5041 taagctacat cagcaatact aactgatttt aatttacttc cgctgctaac taaaaagcgc
     5101 tgttgatagt tttgtgtttg cgtaaaatgg ttgagtaata aatcaatgtt tttagttgga
     5161 tttcctgtgg attgctggaa tttgttgatg gcagcaaaca attcgtcttt gttgatgggt
     5221 tttaacaaat aatcaatact atttactttg aaggccttta tggcgtattg atcaaatgct
     5281 gttgtaaaaa ttaccggagc agtaacttca attttattaa aaatactaaa gcttatatca
     5341 tcacctaaat ggatatcaca aaagagtaaa tcgggtttgt ttttagaaag aaaagcaacg
     5401 gcagaagtta catcgtctag ttttgccata acggtgatat cagcaggaaa actcaaaatt
     5461 aatttttcga gataatctgc tgcaattttt tcgtcctcta taatggcaat gtttatcata
     5521 attcttcggc gacggtttgg cttactaatg ggatttgagc cacatattga ttatcaatga
     5581 gcccaaattt aggaatacta tcacaaatta attgatatcg gcttatcagg ttttgatgac
     5641 caatttgggt ggattgtaat ttttctgctc ttggttggaa tatgttactt actgttaatt
     5701 tgtccccttt agctattatt tgaataacaa gtggttttgc atttgaaatc acgttatgtt
     5761 tgatggcatt ttctactaat agttgtaaag ttaatggcgg gagatagtga tttaaactat
     5821 cggcatcaat ttgcgtttca actatcaaat tatcaccaaa acgtatttta tttaaataaa
     5881 taaacgattg tataaattgg atttcatcat gtaatgtaac taccagctga tcgcgttttt
     5941 ctaaaacata acgataaact cttgctaatt cctgaacaaa ttctttagct ttggtagtgt
     6001 catcttcaat taatgattgc agggcattaa agctgttaaa taaaaagtgg ggactaattt
     6061 gtgtttttaa accttctaat tgtgcacgta aaaattcacg ctccagcttt tctttttctg
     6121 cgattgatat ttttagtttc tccacgtaat gtctgatttc taaaacaagg ctaacaatac
     6181 ttactaaaac ggtggcaaac atttgatttt gaaacacact cattttagct tcctgatcgg
     6241 taagtttata aaataagtgg ttaaatattt gccattcaaa atagataata atattggaaa
     6301 taattaaccc taatcccaat gataaactaa ttcttttaaa tgcagataat ttttcttcat
     6361 acttcgttgt aattgttttt atgatataaa tattagcaag aaataacgaa aatgttacca
     6421 atacagaaaa tatacaagaa atagcatagt ccctggcagt aaggcatggt c
//
LOCUS       DAORKW010000165         6458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_415_length_6458_cov_6.655005, whole genome shotgun sequence.
ACCESSION   DAORKW010000165 DAORKW010000000
VERSION     DAORKW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_415_length_6458_cov_6.655005"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLB46_07915"
     CDS             <1..496
                     /locus_tag="PLB46_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRF76482.1"
                     /translation="ELNYYDKRHLFSLAGEEKHYTAGNRKIFPEVHEWKILPLICYDL
                     RFPVWSRQSPPFTELGAHPYDLLIYVANWPDRRIYAWQQLLIARAIEIQCYVAGVNRV
                     GEDGNGVMHSGHSAIINPMGERQAEIVSEEAVLQYTLSAENLTRVRENLPFLDDRDEF
                     ELRR"
     gene            599..1453
                     /locus_tag="PLB46_07920"
     CDS             599..1453
                     /locus_tag="PLB46_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRF76483.1"
                     /translation="MITDKIIKPNSGYFMLFVFLLMMVGAGFLMATKHPAFALFFIPL
                     FFVLIGFFIVNPNDSKVLVMFGKYVGTVRDNGFYWANPFYSKAKVSLKARTLEGDKIK
                     VNDKLGNPIIIGTVIVWQVTDTAKAKFEVENYEMYVKLQSDTAVRHLAGTFSYDHFGD
                     DESKEITLRGGGDHINEMLMGELKDRLSLAGVQVIEARITHLAYSEEIAGAMLQRQQA
                     SAVVAARTKIVEGAVGMVQLALHKLSAESIVELDEEKKAAMVSNLLVVLCSDKAASPV
                     VNTGTLYH"
     gene            1506..2012
                     /locus_tag="PLB46_07925"
     CDS             1506..2012
                     /locus_tag="PLB46_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76484.1"
                     /translation="MYTEEQKINNRFAIIIFITAVFICVAGLFFAAYQIVQQKGDLKP
                     IYIGSAAMAGVIMLIYFIFFRTTLTIAIDAYRITIKFYSLFRKNLDIEHSNISTWEYR
                     KLKMIMDYGRIGYSTDPILKKTGFIMNGKNVFEITLASGRKIGFTAANAEMMMTAFKT
                     YIPNKEKR"
     gene            2012..2188
                     /locus_tag="PLB46_07930"
     CDS             2012..2188
                     /locus_tag="PLB46_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HRF76485.1"
                     /translation="MAKEEKKQTGKDRQAFILRISPEILKSLEQWAADDFRSVNGQIE
                     YLLNEALKKAGRRK"
     gene            complement(2342..2974)
                     /locus_tag="PLB46_07935"
     CDS             complement(2342..2974)
                     /locus_tag="PLB46_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76486.1"
                     /translation="MNYFLPLEANGVYHIFNHANGWDDFYYNEQNCDYFLKKLRYHLW
                     PVLNVCAYNLLLNHFHLIVRIRSETEIIRCSKLWCRHQRVPYRPLKPAQQFSNFFNGY
                     AKAMNKVYERKGSLFQRQFKRILIDNDDYLRYVIAYVHTNAQKHNMVDDFRNWEFSSY
                     HKILSDKPTYLLKEKVLTLYNGEQNYINYHENFLYARNVASIKYDNFTGR"
     gene            3210..4694
                     /locus_tag="PLB46_07940"
     CDS             3210..4694
                     /locus_tag="PLB46_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76487.1"
                     /translation="MKKNDDLYKLIKSLEKPEKGYFKKFAQTYSKTADQQYLQLFDAM
                     DAQKEYDEDAIIKKFRNTSLSTQISVAKNYLWELILKSQRAYRANTSKFMRLNALMEN
                     GEILFEKGLYQQALLTWEKAKKLALDFDEKAFILDIEAWKRRYFVDLRADLWDQNVLP
                     SFETTDKLLHQYSNTLAIQKIYFKSTKYIKTNPDFRNPEHKKEWDAIMKNPILNPENE
                     PEDFYGKLYFNYVHSNYHHLSNNEEQNLFFISKNIKLWESNPLLIEVEPIRYIAAVNN
                     YLGTLSKFSRYQAFIDYVEAFKPPRFNSINQEAIYFEHWWLWKEITFKITGNDVAFGN
                     FIQETFPDLLKYAPYINSVRWMLIRFGIAINYFKTGRFDEALTLTNELIDTKDIELRK
                     DIQAHTRMMYLILHYELKNNLILESLSRSVKRYLIQNENYFETEKVFIKFFNKLINAS
                     DKAEAEHIINDLDEEVNAIFNNNKSEKIAFMTMPLTEWLKIRKM"
     gene            complement(4750..6345)
                     /locus_tag="PLB46_07945"
     CDS             complement(4750..6345)
                     /locus_tag="PLB46_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76488.1"
                     /translation="MTRSIFILLFTFSSTLMFAQPQWLKGFGDENSNYFSGMDVDPSG
                     NIYLAGVFSDSISLGGITAYGGSYDLYIAKFNPDGEILWLKAETSDARYDLYDLKISG
                     DDGFVICGSFDTDDAVFGDITLTQSDFGNTYVVKYDNNGSPVWAKTEIGYAYYEHLAI
                     DSNGDIIITGSLVGDFTFDGTLQESFGEYDILYAKYSVSGEALWIKVMGGEGYEYSAE
                     VITDAENNIIMTGRFNMPFEFEGNVFNLIALGDNFVMKTDADGTPLWVNLIASENDDS
                     EIKGMAIGADGNLYLGVSVENNTYVDAITINTLGLIDACLMQLNPDNGALNWYKTGGG
                     YEYDYVYALEVGSEGVFIGGVYSGTAEFDGLQLPDAFSGSYITSYDYNGDATQVVSFV
                     GESFVSLQEIEVDQHGNMYAGGSFYDDVVFDGLDPLATEGSVDIFLYKEASPLVAINN
                     YAGTINVSITPNPATTVINLNVVNETNYQLDIINQQGAIVKSIENAVGENSMYIADLA
                     AGMYFVVITTTDGVKTSQPFVKN"
BASE COUNT         2218 a         1133 c         1093 g         2014 t
ORIGIN      
        1 cgaacttaat tattacgata agcgccattt gttttcatta gccggtgaag aaaaacatta
       61 tacagcgggc aacagaaaaa tatttccgga agtgcatgaa tggaaaatat taccgcttat
      121 ttgttatgat ctacgttttc cggtttggag cagacaatcg ccaccgttta ccgaactggg
      181 agctcatcca tacgacctgt tgatttatgt agccaattgg cctgatagaa gaatatatgc
      241 ctggcagcag ctgctaattg cacgtgctat tgagatccaa tgttatgtag ccggggttaa
      301 cagggttggc gaagatggca atggagttat gcactcaggg cactccgcca tcatcaatcc
      361 tatgggcgaa cgccaggctg aaatagttag tgaagaagct gttttacaat ataccctttc
      421 ggctgaaaac ctcaccagag taagagaaaa cctacctttc ctcgacgacc gcgacgagtt
      481 cgagttacgc cgctaaatgt taaaattgta ttaaatgtaa aatattccat ggttttgtgt
      541 cgtctggtat gatatctttg tgatatcatt ttaaaatcag tcaaaaaaat ttaaaactat
      601 gataaccgac aaaatcatta aacctaattc agggtatttt atgttgtttg tgttcttact
      661 catgatggta ggcgcaggct ttttaatggc gaccaaacat ccggcatttg ccttattttt
      721 tattccgcta ttttttgtgt taattggatt ttttattgtt aatccgaacg actcaaaagt
      781 attagtcatg tttggaaaat atgttggaac ggttcgcgac aatggatttt attgggcaaa
      841 tccattttat tcaaaagcga aagtttcatt aaaagcacga acattagaag gtgacaagat
      901 taaagtaaac gacaaattgg gtaatccaat cataattgga accgtaattg tttggcaggt
      961 tacagatact gctaaagcaa aatttgaagt ggagaattac gagatgtatg taaaattaca
     1021 atctgataca gcagttcgtc atttagccgg cacattttct tacgaccatt ttggtgatga
     1081 tgaatcaaaa gaaattacct tgcgtggtgg tggcgaccac attaatgaaa tgttgatggg
     1141 tgaattaaaa gacagattaa gtttagccgg tgtgcaagta attgaagcaa gaataacaca
     1201 tctagcttat tcggaagaaa ttgcaggggc aatgttacag cgtcagcaag ctagtgctgt
     1261 agtagcagca agaaccaaaa ttgttgaagg tgctgtaggc atggtgcaac tagcattaca
     1321 taaattatca gctgaaagta tagttgagtt ggatgaagaa aagaaggcag ccatggttag
     1381 taatttacta gtggtattgt gttcggacaa agcagcaagt ccggtagtaa atacaggaac
     1441 cttatatcat taattaattt tttgcagcga ataaagttgt attcgctgca atttatttat
     1501 gcattatgta tacagaagaa cagaaaataa ataatcgttt tgcaataata atttttatta
     1561 cggctgtatt tatttgtgtt gcaggtttat tttttgcagc ttatcaaatt gttcagcaaa
     1621 aaggtgattt aaaaccgatt tatattggaa gtgctgcaat ggccggtgtt attatgttga
     1681 tttattttat ttttttcagg accactttaa caattgctat tgatgcctat aggataacaa
     1741 taaaatttta ttcattattt agaaaaaacc tggacataga acacagcaac atttcaactt
     1801 gggagtatcg caaactaaag atgataatgg attatggcag aattggttat agcacagatc
     1861 caattttaaa aaaaacgggg tttatcatga atggaaaaaa tgtttttgaa attacgttag
     1921 ccagtggacg gaaaattgga tttactgcag caaatgcaga aatgatgatg acggctttca
     1981 aaacatatat acctaataaa gaaaaacgtt gatggcgaag gaggaaaaaa aacaaaccgg
     2041 taaagaccgt caggccttta tacttcgtat ttcacccgaa atattgaagt cgttggagca
     2101 atgggctgcc gacgatttcc gcagtgttaa tggtcagata gagtatttat taaatgaggc
     2161 tttgaagaag gctgggagga ggaaatgagt ggggggtttt tgctgttttc tgcgcctgca
     2221 attttggcag atgttataca cctgtcaggt ttaaacctgc gaggtttaaa cctgacaggt
     2281 tgtttgtcga taaccgctta attcctctcc ctaatttaga ttcagccaaa tttatcaaat
     2341 ttcacctccc cgtaaaattg tcatacttaa tagatgcaac atttcgagca taaagaaaat
     2401 tttcatgata atttatatag ttctgttcgc cattatataa ggttaacact ttttctttta
     2461 acaaataggt aggtttatca ctaaggattt tatggtagga actaaactcc cagtttctaa
     2521 aatcatcaac catattatgt ttttgggcgt tggtatgcac ataagcgata acatacctca
     2581 aataatcgtc attatcaatc aaaatccgct tgaactgtct ttgaaataaa ctaccttttc
     2641 gctcgtaaac tttattcata gccttagcat aaccattgaa aaaattacta aactgctgcg
     2701 ctggtttcag tggtcggtat ggaacgcgct gatgtctgca ccataactta ctacatcgaa
     2761 taatttcggt ttctgaccta atacgaacta tcaaatgaaa atgattgagc aaaagattgt
     2821 aagcacatac attcaacacc ggccataaat gatatctcaa ctttttcaaa aaataatcac
     2881 aattctgctc attataataa aaatcatccc aaccatttgc gtggttaaaa atatggtaaa
     2941 ctccatttgc ttctaagggt aaaaagtaat tcatttacca aaaatagcag cactttattt
     3001 tcataacata aatacattaa tcaggtatgt ggattactca ttaaaaacac taattactat
     3061 tcgcatctaa gcggatagtg ttttaaaaat aaaaagaata gccgcttaac agcaattttt
     3121 ttttcgatgt atataaatat tttgttaatg tatattaatt ttcaaaattt cagtctgaat
     3181 catatacctt tagttaacat tccggcatta tgaagaaaaa cgatgatttg tataagctga
     3241 ttaaatcact tgaaaaacct gagaaagggt atttcaaaaa atttgcgcag acttattcaa
     3301 aaaccgctga tcaacaatac cttcagttgt tcgatgccat ggatgcacaa aaagaatatg
     3361 acgaagatgc aattattaaa aaatttagaa atacttcatt gtcaactcaa atttcagttg
     3421 ccaaaaatta tttatgggaa ttaattctaa aaagtcagcg cgcctatcgt gctaatactt
     3481 caaaatttat gcgactaaat gctctaatgg agaatgggga aatattattt gaaaagggtt
     3541 tatatcaaca agccttactt acctgggaaa aggctaaaaa attggcgctt gattttgatg
     3601 aaaaagcatt tattttagat attgaagcct ggaaacggag atattttgtt gatttacgcg
     3661 ctgatttatg ggatcaaaat gttttgccct catttgaaac aaccgataaa ttgttacatc
     3721 aatatagcaa tacattagcc attcaaaaga tttattttaa aagtacgaag tacattaaaa
     3781 caaatcccga tttccgaaac cctgaacata aaaaggaatg ggatgcaatt atgaaaaacc
     3841 ccatcctcaa cccggaaaac gagccggaag atttttatgg gaaactgtat tttaattatg
     3901 tgcattctaa ttatcaccat ctttctaata atgaagaaca aaatcttttt ttcatcagta
     3961 aaaatattaa attatgggaa tcaaacccgt tactaattga agttgaacca atacgatata
     4021 tcgctgcagt aaataattat cttggaacct tatctaaatt cagcagatat caggcattta
     4081 ttgattacgt tgaagctttt aagccaccac gttttaattc aattaatcag gaagcaattt
     4141 attttgaaca ctggtggctt tggaaagaaa taacttttaa aattaccggc aacgatgttg
     4201 catttggaaa ctttattcag gaaacatttc ccgatttatt aaaatatgca ccttatatca
     4261 atagcgtaag atggatgctt atccgttttg gtattgccat aaattatttt aaaaccggtc
     4321 gttttgacga agcgcttaca cttactaatg agttaataga tacaaaagat attgaattga
     4381 gaaaagacat tcaggcacat accagaatga tgtatttaat tttacattat gaattaaaaa
     4441 ataatcttat tctggaaagc ctttcccgct cagttaaacg ttatcttatt caaaacgaaa
     4501 attattttga aaccgaaaag gtttttatta aattttttaa taaacttatt aatgcgtcgg
     4561 ataaagcaga agcagaacac attattaatg atttagatga agaagtaaat gcaattttta
     4621 acaataataa aagtgaaaaa attgcattta tgacgatgcc attaaccgaa tggttgaaaa
     4681 taagaaaaat gtaaaaaccc cgatgaaaat gaattccacc ggggtctgac tgtagcggtg
     4741 cagtgctaat taatttttca caaatggctg gcttgtttta acgccatccg tagttgtgat
     4801 aactacaaaa tacattcccg cagccaggtc tgctatatac atggaatttt caccaactgc
     4861 attttcaatt gatttaacaa ttgcaccttg ttgattgata atatccaatt ggtaatttgt
     4921 ttcgtttact acgtttaaat ttataactgt tgttgccggg tttggtgtaa ttgaaacatt
     4981 aatggttccg gcataattat taattgcaac cagcggagat gcctctttat aaagaaaaat
     5041 atctacacta ccctccgtag ctaatggatc tagaccatca aaaacaacgt catcataaaa
     5101 acttccacct gcgtacatat tgccatgttg gtctacttct atttcctgaa gactcacaaa
     5161 actttctcca acaaaagaaa ctacttgtgt tgcgtcaccg ttataatcat acgaagtaat
     5221 ataacttcct gaaaaagcat caggtaattg taatccgtca aattcggcag ttcctgaata
     5281 aacccctcct ataaaaacac cttcactgcc aacttccaac gcgtatacat aatcatactc
     5341 ataccctcca ccggttttat accaatttaa ggccccatta tcaggattta attgcattaa
     5401 acacgcatca atcaatccta aagtgtttat ggtaatggca tcaacatatg tattattttc
     5461 taccgaaaca ccaaggtata aattaccatc agcgccaata gccatacctt taatttctga
     5521 gtcatcattt tctgatgcaa ttaaattcac ccacaatggc gtaccatcag catctgtttt
     5581 cattacaaaa ttatcaccaa gcgcaatcag attaaacaca ttaccttcaa attcaaaagg
     5641 catattaaat cttcccgtca tgataatatt attttctgca tcggttatta cttctgcgga
     5701 atattcatat ccttcaccac ccattacttt aatccaaagc gcttcaccgc taactgaata
     5761 ttttgcatat aaaatatcat actcgccaaa tgattcctga agtgtgccat caaaagtaaa
     5821 atcaccaact aaagaacctg ttataatgat atcgccattt gaatcgatag ctaaatgttc
     5881 ataataagca tatcctattt ctgttttagc ccataccggt gatccattgt tatcatactt
     5941 tactacgtag gtattaccaa aatcagattg ggttaaagta atatctccaa aaacagcatc
     6001 atctgtatca aatgaaccac aaattacaaa accatcatcg ccggaaattt taagatcata
     6061 caaatcatat cgtgcatctg atgtttctgc tttaagccaa aggatttcac catcaggatt
     6121 aaatttagca atatacagat cgtaacttcc gccataggcc gtaattccac ctaacgaaat
     6181 actatccgaa aaaacacctg ccaaataaat gtttccactc gggtcaacat ccattccgct
     6241 aaaataattt gagttttcat ctccaaaacc tttcaaccat tgcggctgag caaacatgag
     6301 tgtgcttgaa aaagtaaata ataaaataaa aatgctacgt gtcataacct tgagtttttt
     6361 tgttaaaaaa tagaaagtga aatccgttta ccgtaaaaac actgcacagt gaccgataaa
     6421 cggctattta cttcccttga acaacacaaa ggtcataa
//
LOCUS       DAORKW010000166         6451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_416_length_6451_cov_6.128518, whole genome shotgun sequence.
ACCESSION   DAORKW010000166 DAORKW010000000
VERSION     DAORKW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_416_length_6451_cov_6.128518"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PLB46_07950"
     CDS             <1..755
                     /locus_tag="PLB46_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HRF76489.1"
                     /translation="TDMVMYTVGPAAFLYVLFEMRKYDGAARKKLTAALIFIVFSIFF
                     WAFFEQSGGSLALFAFNNLHDSLFGVNIDPNVVNNSANSLFVIIFAPLLGLVWVYLAK
                     RKKEPNSAVKFGLGFLLLAAAFYVFYYTINFANEQGLTSLEIFTLGYFIITFGELCLS
                     PIGLSLMTKLSPQPMQGLMMGMWFLASAYGQYAAGLLGAGMSVASEDASAYEKLVSYT
                     NGYQQLAIYAVVAGAALLLLSPVIKRLMGSTR"
     gene            846..1649
                     /locus_tag="PLB46_07955"
     CDS             846..1649
                     /locus_tag="PLB46_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRF76490.1"
                     /translation="MEILGYIGALLVGLTLGLLGAGGSILTVPVLYFLFDVDAELSTA
                     YSLFIVGLTALIGAVPNMKRKTISYKTAIIFSIPSLTAVYLTRTYLLKAIPDNLLEIG
                     SFTLTKDIGLLLFFAIIMIVAAISMINQRKIVDPVYDEKQHFNYPMIIAEGLVIGTLT
                     GLVGAGGGFLIIPALVVFAKLPMKMAVGTSLLIIAIKSLVGFLGDIQTGQLIDWTFML
                     VFSAVTISGILIGTWLNKYVDGKKLKGAFGWFVLLMGIFIIIEELFFEI"
     gene            1680..2204
                     /locus_tag="PLB46_07960"
     CDS             1680..2204
                     /locus_tag="PLB46_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF76491.1"
                     /translation="MSKKMIIAGILILIVAIFVTFKNVQAQAHVESNSFDLMLSTLLS
                     HDVPELGIKDIPSDSNIIYLDAREKSEYDVSHIKNAIWVGYDTFNADQLANLDREATY
                     IVYCSVGYRSEKITHQLQVNGFLFVSNLYGGIFEWVNQNLPVYDNTDNATTKVHGYSP
                     KWGIWLNKGEKVYE"
     gene            2298..3185
                     /locus_tag="PLB46_07965"
     CDS             2298..3185
                     /locus_tag="PLB46_07965"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRF76492.1"
                     /translation="MDKYIDIFLQSFSGYWKYLSHEILNPHWGNYFYWLIGISLLVWM
                     LEILFPWRKEQKIIREDFWMDAFYMFFNFFLFSLIGFYAVSNVFVQLFNDFLALFGIK
                     NGIAIEINALPFWLKITFAFILKDFIQWNVHRLLHANKYLWQFHKVHHSVKQMGFAAH
                     LRFHWMENIIYRSIQYIPLAMLGFGINDFILANLIALVIGHFNHANINLPLGPLKYIL
                     NNPQMHIWHHAKYIPADKPKGINFGISLSIWDYLFKTDYIPHTGRDIELGWPGDEQFP
                     KDFIHQEIYPVGEGKEGKN"
     gene            3593..4309
                     /gene="pyrH"
                     /locus_tag="PLB46_07970"
     CDS             3593..4309
                     /gene="pyrH"
                     /locus_tag="PLB46_07970"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRF76493.1"
                     /translation="MSLKYKRILLKLSGEALMGNQNFGIDPTRLQQYAKDIKTASDAG
                     VQIAIVIGGGNIFRGMSGVGSGIDRAQGDYMGMLATVINGMALQSALENVGLYTRLLS
                     AIKMEQICEPYIRRRAIRHLEKGRIVIFGAGTGNPYFTTDTAASLRAIEVEADVILKG
                     TRVDGVYTADPEKDPTATKFDTITFREVISRNLNVMDMTAFTLCEENNLPIIVFDMNK
                     QGNLEKLISGENVGTLVAGK"
     gene            4345..4911
                     /gene="frr"
                     /locus_tag="PLB46_07975"
     CDS             4345..4911
                     /gene="frr"
                     /locus_tag="PLB46_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRF76494.1"
                     /translation="MHEDELSLTIDMAQETMQKAIKHLEVELSHVRAGRATLAILDNV
                     RVDYYGAPTALHQVSNLSTPDARTITIQAWDKKMLGEIEKAILAANIGLTPVNNGDII
                     RLIMPPLTEERRRDLVKQVRHMGENTKVAIRNIRKDANEDIKKLQKEGLPEDMAKDGE
                     DRVQKLTDKFIAEVDKHLTIKEQEIMTV"
     gene            4948..6345
                     /gene="fumC"
                     /locus_tag="PLB46_07980"
     CDS             4948..6345
                     /gene="fumC"
                     /locus_tag="PLB46_07980"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRF76495.1"
                     /translation="MNYRKEKDTMGVVEVPTDVYWGAQTQRSIMNFPIAQDINKMPKE
                     IIAAFAYLKKAAALTNHQLGVLDKDKCDVIGKVCDEILAGKLNEQFPLVVWQTGSGTQ
                     SNMNVNEVVAYRGHVMMGGDLMDEKKAIHPNDDVNKSQSSNDTFPTAMHIAAYKILIE
                     NTIPGIEKLRDTLEAKSKAFMHVVKIGRTHFMDATPLTLGQEMSGYVSQLNHGLKAIK
                     NTLEHLSELALGGTAVGTGINTPDGYAELVAKNIATLTGLPFITAENKFEALAAHDAI
                     VEAHGALKTVAVSLMKIANDIRMLSSGPRSGIGEIHIPDNEPGSSIMPGKVNPTQCEA
                     LTMVAAQVMGNDVAINIGGATGHFELNVFKPVMIYNFLHSARLIGDACVSFNDNCAVG
                     IEPIEANIKKHVDNSLMLVTALNTKIGYYKAAEIAQTAHKQNKTLKQVAVELGYLTPE
                     QFDEWVIPMNMVGKI"
BASE COUNT         2015 a         1125 c         1318 g         1993 t
ORIGIN      
        1 ataccgacat ggtaatgtac actgttggac ctgctgcatt tttatatgtg ttgtttgaaa
       61 tgagaaaata tgatggtgcc gcaagaaaaa aattaactgc agcattaata tttattgtat
      121 tctctatttt cttctgggct ttcttcgaac aaagtggtgg ctcattagca ttattcgcat
      181 ttaataattt acacgattcg ttatttggtg taaatatcga cccgaacgta gttaataact
      241 cagcaaactc attgtttgta atcatatttg ctccgcttct tggtttagtg tgggtgtatt
      301 tggccaaacg taaaaaagag cctaattcag cagttaaatt tggtttagga tttttattac
      361 tcgcagctgc attttatgtg ttttattaca ccattaattt tgccaacgaa caaggtttaa
      421 catctttaga aatctttaca cttggttatt tcatcattac atttggagag ttatgtttgt
      481 cgccaatcgg tttgtcgctc atgacaaaat tatctcctca acctatgcag ggtttaatga
      541 tgggtatgtg gtttttggca agtgcttatg gtcaatatgc tgccggctta ttaggagcag
      601 gtatgagcgt ggccagcgaa gatgcttcag cttatgagaa actggtatcc tataccaatg
      661 gttatcagca attagccatt tatgcggttg ttgcaggtgc ggcgctcttg ttactgtcac
      721 cggtaatcaa acgtttaatg ggaagtacac gataaatcat gggcaaggta acaattgtta
      781 caacacccta taatgaataa acttattttt gcaccgcctc agcttttatt tgaggcggtt
      841 tttttatgga aatattagga tacataggcg ctttattggt gggactaaca ctcggtcttt
      901 taggagccgg tggtagcatc cttactgttc cggttttgta ttttttattt gatgtagacg
      961 cggaactgag cacggcctat tcacttttta ttgtgggact tactgcttta attggtgcgg
     1021 tgccaaatat gaaaagaaaa acaatcagct ataaaacagc catcattttt tctattccca
     1081 gtttaacggc agtgtattta acaaggactt atttgttaaa agcgattccg gataatttat
     1141 tggaaattgg gtccttcacg ctcacaaaag atataggatt attattgttt tttgcaatta
     1201 tcatgattgt tgccgcaatt agtatgatta atcagcgcaa aattgttgac cctgtttacg
     1261 acgaaaaaca acattttaat tacccaatga ttattgcaga aggtttggtt attggtactt
     1321 taaccggttt ggttggtgcc ggcggcggat ttttaattat tccggcactg gtagtttttg
     1381 caaaattgcc gatgaaaatg gcagtaggaa catcgctttt aataattgcc ataaaatcgt
     1441 tggtcggatt tttaggcgat attcaaacag gccaactcat agactggaca tttatgctcg
     1501 ttttttcagc ggtaaccatt tcgggcatac ttattgggac ttggttaaat aagtatgtag
     1561 atggtaaaaa gttaaagggt gcctttggct ggtttgtgtt attgatggga atttttatta
     1621 ttattgaaga attatttttt gaaatataat gttatcatta agtgccttat caatttaaaa
     1681 tgagtaaaaa aatgattatt gccggtattc tgattttgat tgtagcgatt tttgttacat
     1741 tcaaaaatgt gcaggctcag gcacatgttg aaagcaatag ttttgacctg atgttatcta
     1801 ctttgttatc gcacgatgtg ccggaattgg gcataaaaga tattccttcc gattcaaata
     1861 ttatttacct cgatgcccgc gaaaaatcag aatatgatgt tagtcatatc aaaaatgcta
     1921 tttgggtggg ttacgatacg tttaatgccg accaattagc aaatttggat agagaagcaa
     1981 cctatatcgt ttattgttct gttggatacc gctcagaaaa aataacgcat caactgcagg
     2041 taaacgggtt tttatttgta tctaacttat atggtggaat ttttgaatgg gtaaatcaaa
     2101 atttacctgt ttacgataat accgacaatg ccacaaccaa agtacatggc tattccccca
     2161 aatggggcat ctggctcaat aagggcgaaa aagtgtacga gtaatttaca aaattattta
     2221 cagtgttatg tgatattcgt cacccatagc aactcaaggc ctgtatatct ttgtagttca
     2281 acagcacaaa atataatatg gataagtata ttgatatttt tttgcaatcc ttttctggtt
     2341 attggaaata tcttagtcat gaaatattaa atccccattg gggaaattat ttttattggt
     2401 tgataggcat ttcattattg gtgtggatgc tggaaatatt atttccttgg cgtaaagagc
     2461 aaaaaattat ccgtgaagat ttttggatgg atgcatttta catgtttttc aatttcttcc
     2521 tgttttcgct cattggtttt tatgcagtgt ctaacgtttt tgtgcagttg tttaatgatt
     2581 tccttgcgtt gtttggaatt aaaaacggaa ttgctatcga aataaatgct ttaccatttt
     2641 ggctaaaaat tactttcgca tttattttaa aagattttat tcaatggaat gtgcatcgtt
     2701 tattacatgc caataaatat ttatggcaat ttcataaagt gcaccacagc gtaaaacaaa
     2761 tgggatttgc agcgcacctg cgtttccact ggatggaaaa tattatttat cgctccattc
     2821 aatatattcc tttagccatg ttaggatttg gcatcaacga ttttatcctg gctaatttaa
     2881 ttgcattggt gataggtcat tttaatcacg caaatattaa tctcccttta ggaccattaa
     2941 aatatatttt gaataatccg caaatgcata tttggcatca cgcaaaatat attcctgccg
     3001 ataaaccgaa aggtatcaat ttcggtatca gcctgagtat ttgggattat ctcttcaaaa
     3061 ccgactacat ccctcacacc ggccgcgaca tcgaattagg ctggcccggc gatgaacaat
     3121 tccctaaaga ttttatccat caggagattt atccggttgg ggaagggaag gaggggaaga
     3181 attgatacag ccattgagca atcccgatag ctatcgggag aagcaacatt gagcaagatt
     3241 gatgattgaa acagccataa agcaatcctg atagctgtca ggagaacaag attggctaat
     3301 cttggcttct aatcttatcc cgtattccat tccaccaccg aacccggctg tttccgcctc
     3361 ggcggacgtt ccgttcattg gtacattcgc acatcggtac attggtacat caacctgctt
     3421 cgtcttgacg tttttaacag gtcaacacgt gggttgacca caaccgcagc tctgaattcc
     3481 attccactta agaacccggc tgtttcattg gtacatcggt acattcacac attggtacat
     3541 taacatccgc cgcagcggac acattagcac attaagtatt aactttgccc cgatgagtct
     3601 gaaatacaaa cgaatactcc tgaaactcag tggcgaagca ctgatgggca accaaaactt
     3661 cggaattgac cctacccgtt tacaacaata tgcgaaagac attaaaaccg cttctgatgc
     3721 aggcgttcaa attgcaattg taattggagg tggtaatatt tttagaggaa tgagcggcgt
     3781 tggttccggt attgatcgcg ctcagggcga ttatatggga atgctggcga ctgttattaa
     3841 tggcatggcc ttacaaagtg ccctcgaaaa tgtggggtta tacaccagat tgctctctgc
     3901 aattaaaatg gaacaaattt gtgaacctta tatccgccgt cgtgcgattc gtcaccttga
     3961 aaaaggtcgt atcgtaattt ttggcgcagg aacaggtaac ccttatttca caaccgatac
     4021 tgcagcaagt ttaagagcta ttgaagttga ggcagatgta attttaaaag gcaccagggt
     4081 agatggcgtt tatacggccg acccggaaaa agaccctaca gcaactaaat ttgataccat
     4141 tacattcaga gaagttattt cccgtaatct taatgttatg gatatgaccg catttacatt
     4201 atgcgaagaa aataacttgc cgattattgt tttcgatatg aacaaacaag gtaatcttga
     4261 aaaattaatc agcggcgaaa acgtcggtac attagtagct ggaaaataat tacaattaaa
     4321 actgataaat aaaatatata agttatgcat gaagatgaat taagtctgac gatagacatg
     4381 gcgcaggaaa caatgcaaaa agccatcaaa catcttgaag tagaattgtc gcacgtaaga
     4441 gcaggaagag ctacattggc tattctggat aatgtgcgtg tcgactatta tggtgcaccc
     4501 acggctttgc atcaggtgag taatctttca acccccgatg caagaacaat tactatccag
     4561 gcttgggata aaaaaatgct tggcgaaatt gaaaaagcaa ttcttgccgc aaatattgga
     4621 ctaacgccgg taaataatgg cgatattatt cgtttaatta tgccaccact caccgaagag
     4681 cgtcgccgcg atttagtgaa acaagttcgc cacatgggtg aaaatacgaa agtagctatc
     4741 cgcaatattc gcaaagatgc caacgaggat atcaaaaaat tacaaaaaga aggtctccct
     4801 gaagatatgg caaaagacgg tgaagaccgc gtgcaaaaac tcacggataa atttattgct
     4861 gaagtggaca aacatctcac aataaaggaa caagaaataa tgactgtata acatttgcta
     4921 atttgctaat cagctaattg cttaattatg aactatcgta aagaaaaaga caccatgggc
     4981 gtggttgaag ttcccaccga tgtgtattgg ggcgcacaaa ctcagcgttc tatcatgaat
     5041 tttcctattg ctcaggatat aaataaaatg cctaaagaaa ttattgcagc tttcgcttat
     5101 ttaaaaaagg ctgctgcatt aaccaatcat cagttaggcg ttttagataa agataaatgc
     5161 gatgtcatcg gaaaagtttg cgatgaaatt ttggccggaa aattaaacga acaatttcct
     5221 ttagtagttt ggcaaactgg ttcaggtacg caaagtaata tgaatgtaaa tgaagtggtt
     5281 gcgtatcgcg gacatgtgat gatgggtggt gatttaatgg atgagaaaaa agctattcat
     5341 ccgaatgatg atgtaaataa atcgcaatca tccaatgata cttttccaac agcaatgcat
     5401 attgcagcgt ataaaatatt aattgaaaat accattcccg gcattgaaaa attgcgtgat
     5461 actttagaag caaaatcaaa agcatttatg cacgtggtga aaattggtcg gacccatttt
     5521 atggatgcca caccattaac acttggtcag gaaatgagtg ggtatgtttc tcaactcaat
     5581 cacggattaa aagctattaa aaatacatta gaacacttaa gcgaactggc tttaggtggt
     5641 actgctgttg gaaccggaat taatacacct gatggttatg ctgaattagt ggcaaaaaat
     5701 atcgcaacat taacaggatt accttttata actgcagaaa ataaattcga agctttagct
     5761 gctcacgatg ccatagttga agcacacggg gcattaaaaa ccgttgccgt ttcgttgatg
     5821 aaaatcgcca acgatattcg tatgttgtcg agcggaccac gtagtggaat tggtgaaatt
     5881 catattccgg ataacgaacc tggttcatct attatgccgg gtaaagtaaa cccaacacaa
     5941 tgtgaagcac ttacaatggt agctgcacag gtaatgggaa atgatgttgc aattaatatc
     6001 ggcggagcta caggccattt cgaattaaat gtattcaaac ctgtaatgat ttataatttt
     6061 ttacacagtg cacgtttaat tggagatgca tgtgtttcgt ttaacgataa ttgtgcagta
     6121 ggtatagagc caattgaagc gaatattaaa aaacacgtag ataattcatt aatgctggta
     6181 acggctttaa ataccaaaat tggttattat aaagctgccg aaattgcgca aactgcgcac
     6241 aaacaaaaca aaacattaaa acaagtggca gttgagttag ggtacctcac ccctgaacaa
     6301 tttgatgaat gggtgatacc aatgaacatg gtaggaaaaa tttaaatgat atcgtattga
     6361 aataataaag gctgattata tccaatataa tcagcctttt tatttaggca acaaaaccac
     6421 cgtcgtagac ggtggtcagg agtaaaggta a
//
LOCUS       DAORKW010000167         6425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_422_length_6425_cov_5.549137, whole genome shotgun sequence.
ACCESSION   DAORKW010000167 DAORKW010000000
VERSION     DAORKW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_422_length_6425_cov_5.549137"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="PLB46_07985"
     CDS             <1..1362
                     /locus_tag="PLB46_07985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRF76496.1"
                     /translation="AFNQSSQQFSGGEYTMAPLDEMSPEQRASIFQMLEENEAKLQAE
                     GKLPMVYNKTATVALQFPLAWNDGFTGYNFYAISNYVDHDNAYPNSLEDWNCGERTYD
                     TESGYNHQGIDYFLWPFDWNLTNAGAVKIVAAAPGTIVGKYDGNFDQNCAFNPGSWNA
                     IYVKHTDGSTAWYGHMKKSSLTAKGLGETVEAGEYLGTVGSSGNSTGPHLHFEMYNDD
                     NNLIDPFEGTCNTMNVDTWWADQDPYIKPEINRVQTHTAPPEFMPCPEPAITHESNNF
                     MPGDECYFVFYGKDLSPTDLCNLTITKADGEVWYDWDFYQPDYYLASYWYWWYTLPND
                     APEGEWTWSVELNGTNYEHTFIVGDMPVSADNNNLLNNATAWYQNNTINVQLHTDEMV
                     SANIQLLNTVGQAVAPQVMVSGAEQQDVIIHTNGLSAGLYYVAIQDLPTQSFTTIPVY
                     VYE"
     gene            1355..2263
                     /locus_tag="PLB46_07990"
     CDS             1355..2263
                     /locus_tag="PLB46_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF76497.1"
                     /translation="MSKLIVAFICSFIMFAPESIAQIPVPTKGTIVRHEMFASSFVDA
                     RNIDVWLPENYDVNKKYAVLYMHDGQMLYDAAITWNKQEWQVDEIVGQLLSQNQIIDC
                     IVVGIWNNGVYRHSEYFPQTPISYLTKDFQDTLIKNELQQQPRSDAYLKFVVTELKPF
                     IDKTYSTYTDKNHTMIAGSSMGGLISMYAICEYPEVFGGAACLSTHWPGSLQAPENVI
                     PQAFEDYLSKHLPSPDSHKIYFDFGTTTLDQLYEPHQIKVDSIMVEKGFTEKNWITRK
                     FEGEPHTEEAWSKRLHIPLTFLLGLE"
     gene            complement(2264..3178)
                     /gene="fmt"
                     /locus_tag="PLB46_07995"
     CDS             complement(2264..3178)
                     /gene="fmt"
                     /locus_tag="PLB46_07995"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRF76498.1"
                     /translation="MAKKLRIVYMGTPDFAVAGLQALVEAGHQVVAVITAPDKPSGRG
                     LQIQQSPVKQFAVAHNIPVLQPSKLKDPEFITALKSYHADLQVVVAFRMLPEIVWNMP
                     RLGTLNLHASLLPDYRGAAPINHAIIQGEKITGVTTFFLQHAIDTGDIIFMEKMAIEE
                     NDNAGTLHDKLMHLGATLIVKTVNAIEAGNYVTTPQSGTSDKIAPKIFKETCLINWNQ
                     PTATIYNFIRGLSPYPGAYCNVNNKVYKILEASKEITDTHLIPGTFIVENATLKFATT
                     DGFIHVLSLQAEGKKRMATGEFLRGNKL"
     gene            complement(3178..4419)
                     /locus_tag="PLB46_08000"
     CDS             complement(3178..4419)
                     /locus_tag="PLB46_08000"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HRF76499.1"
                     /translation="MSIIALAFTLFTVSIACKNNDSAIDNAQPVSNPTEVHKQVNYDT
                     DIITGAQQTELLLPLIKGKYIACVVNQTSTIGDKHLVDSLIASGVNIATIFAPEHGFR
                     GTEDAGATILNAKDTKTGLPVLSLYGNKKKPTQQDLADVDVVVFDIQDVGARFYTYIS
                     SLHYVMEACAELGKPIIVLDRPNPNGYYVDGPLLDKAYSSFVGMHPIPVVHGMTIGEY
                     AKMINGEKWLANGVQCDLTVIPCKNYDHKKMYEVPIAPSPNLKNMRAIYMYPTLCFFE
                     GTNVSVGRGTAKPFEIIGSPYLKNTGFEFTPMPGPGAKSPFLMHTKCYGYDFSAKSMD
                     EVQHGKIDIQLLLKIYQEFEPKDKFFLENNFFNKLAGSAELMMQLKGGLSEEEIRLSW
                     QDDISTFKKVRKQYLLYPDFE"
     gene            4468..5703
                     /locus_tag="PLB46_08005"
     CDS             4468..5703
                     /locus_tag="PLB46_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF76500.1"
                     /translation="MWFEFFITKKIIHNKERSFSRLIIAIARVAVALSLSVMIISTSL
                     VNGFRATISDKVYGFWGHINIAKQSLRNSFDDEPIYRVPEFVEQVGKIDGVTSVQVYA
                     RKAAIIKTKTDIEGIIMKGIGSDFDWQQLNKYIIEGDPIDLTQTDVSRGSLISKSTAD
                     RLGFVVGDSILLHIIDQKENGDYEQMYRKLRVNGIYNTGLEEFDKLFVLVDIRHIQRL
                     NNWSQDQIGGYEVFIDDPEKIDVIEPIVDKKADPFWDVQTIQQIIPSVFDWLNLQKVN
                     EWIILTLMILVALINMVTSLLILILERTNMIGILKALGANNFSIRKIFLLNAANIILK
                     GMLWGNIIGIGLCLLQLQFGLIKLPEASYYVKVAPVQINVIWVLLLNAGTFVISILFL
                     LIPSLFVAKIRPIKAIRFN"
     gene            complement(5705..5875)
                     /locus_tag="PLB46_08010"
     CDS             complement(5705..5875)
                     /locus_tag="PLB46_08010"
                     /inference="COORDINATES: protein motif:HMM:NF037605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HRF76501.1"
                     /translation="MPSLALIENIDYYINKDGLLVFTEKYLLERGYCCGNGCTHCPYN
                     NPTQTSPDNNNK"
     gene            complement(5887..>6425)
                     /locus_tag="PLB46_08015"
     CDS             complement(5887..>6425)
                     /locus_tag="PLB46_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRF76502.1"
                     /translation="DLWTYISMDYFKQRINKDEVGSSAMPHKVNPIDFENAEGNLGIA
                     NAMFEHLSAKLPISRLQRDLTDSTVLRNIGVPMAHTILAFKSISRGLKKLQVNEAKIH
                     DDLDNNWAVIAEAIQTILRRENYPQPYEALKNLTRNGQAITKATMQEFIEGLDVSEDI
                     KEQLRGITPFNYTGMRYF"
BASE COUNT         2007 a         1147 c         1220 g         2051 t
ORIGIN      
        1 gcatttaatc agtcatcaca acaatttagt ggtggtgaat acaccatggc cccactagat
       61 gaaatgtcgc cggaacaaag ggcatccatt tttcaaatgt tggaagaaaa cgaagccaaa
      121 ttacaggccg agggaaaatt accaatggta tataataaaa cagctactgt agccttgcaa
      181 tttccgttag catggaatga tggttttacc ggatataatt tttatgccat atccaattat
      241 gttgaccacg acaacgctta tccaaattca ttggaagatt ggaattgtgg tgaaagaact
      301 tacgatactg aatcaggtta taaccatcag ggaattgatt attttttatg gccgtttgat
      361 tggaatttaa ccaatgcagg agcagtaaaa attgtagcag ctgccccggg aacaattgta
      421 ggaaaatacg atggcaattt tgatcagaac tgtgcattta atccgggatc atggaatgcc
      481 atatatgtaa aacatactga tggttcaact gcatggtatg gacatatgaa aaaaagttcg
      541 cttacagcaa aaggtttagg tgaaactgtt gaagccggtg aatatttagg tactgtaggc
      601 agctcaggaa attcaacagg accacattta catttcgaaa tgtataatga cgataataat
      661 ttgatagacc cgtttgaagg tacttgtaat accatgaatg tagacacctg gtgggctgat
      721 caggacccgt atataaaacc tgaaataaac agagtgcaaa cacatacagc tccgcctgaa
      781 tttatgccat gtccggaacc ggctattaca cacgaatcca acaattttat gccgggcgat
      841 gaatgttatt ttgtatttta tggtaaagat ttatcaccaa cggatttatg taatttaacc
      901 attacaaaag ctgatggcga agtttggtat gattgggatt tttatcaacc tgactattac
      961 cttgcgtctt attggtactg gtggtacacc ctaccaaatg atgctcctga aggtgagtgg
     1021 acatggtctg ttgaattaaa tggtactaat tacgaacata cttttattgt tggtgatatg
     1081 cctgtgagtg cagataacaa taacttactc aacaatgcaa ctgcatggta tcaaaataat
     1141 actattaatg ttcaactgca taccgatgaa atggtaagtg ccaatattca attgctcaat
     1201 acagttgggc aagcagttgc tccacaagta atggtatcag gagctgaaca acaagatgta
     1261 attatccata caaatgggtt gagtgcagga ttatattatg ttgctattca ggatttacct
     1321 actcaatcgt ttacgaccat tcctgtttat gtttatgagt aaattaatag ttgcatttat
     1381 ttgttcattc atcatgtttg ctcctgaaag tatagctcaa atacctgtac cgacaaaagg
     1441 tacaattgtg aggcatgaaa tgtttgcttc ttcttttgta gatgcgcgaa atatagatgt
     1501 ctggcttccg gaaaattatg atgttaataa aaaatatgct gtgctttaca tgcacgatgg
     1561 tcagatgtta tacgatgctg ccattacctg gaataaacag gaatggcagg tagatgaaat
     1621 tgttggacaa cttttatcgc aaaaccaaat tatcgattgt attgttgtag gtatttggaa
     1681 caatggcgta tacaggcatt cagaatattt tccgcaaact cccattagtt atttaaccaa
     1741 agattttcag gatactttaa ttaaaaatga attacaacaa caaccccgca gtgatgctta
     1801 cctgaaattt gtagtcacgg aattaaaacc atttatagat aaaacctact caacatatac
     1861 cgataaaaat cacaccatga ttgcagggtc gagtatggga ggtttaattt ccatgtatgc
     1921 catttgtgag tatcccgaag tatttggtgg tgcagcttgt ttatccaccc attggccggg
     1981 aagtttacaa gcgccggaaa atgttattcc acaagcgttt gaagattatt taagtaaaca
     2041 tcttccttca cctgattcgc ataaaatata tttcgatttc gggactacca ctttggatca
     2101 attatatgaa ccacaccaaa ttaaagtgga tagtataatg gtagaaaaag ggtttacaga
     2161 aaaaaattgg attacccgaa aatttgaagg tgaaccgcat actgaagaag cttggagtaa
     2221 acgactacat attccactca cttttttatt gggactagaa taattataat ttatttccac
     2281 gcaaaaattc gcctgtagcc atccgctttt taccttctgc ctgcaacgaa agcacatgaa
     2341 taaacccatc ggtggtagca aatttcaatg ttgcattttc gacgataaaa gtacccggaa
     2401 ttaaatgagt atctgttatt tcttttgatg cctccagtat tttatatacc ttattgttaa
     2461 cattacaata agccccaggg taaggtgata acccgcgaat aaaattataa attgttgctg
     2521 tcggttggtt ccaattgatt aagcaggttt ctttaaaaat tttcggtgca attttatcgg
     2581 aagtgccgga ttgtggcgta gtaacataat taccggcttc aatggcattt actgttttta
     2641 ctattaatgt cgcaccaagg tgcatgagtt tatcgtgaag tgttcctgca ttatcatttt
     2701 cttcaattgc cattttttcc ataaaaatga tatcaccggt atcgatggca tgttgtaaaa
     2761 aaaatgttgt tactccggta attttttctc cctgaataat agcatgattg attggtgcgg
     2821 ctccgcgata atcaggtaaa agtgaggcat gtaaattaag tgtccccagt cgtggcatat
     2881 tccacactat ttcaggcaac atcctaaatg cgacaacaac ctgtaaatct gcatgataac
     2941 tttttaacgc tgttataaat tccgggtctt ttaatttact cggttgcaaa acaggaatgt
     3001 tgtgcgcaac agcaaattgt tttaccggcg attgttgaat ttgtaatccc ctacccgatg
     3061 gtttatccgg agctgttatt acagcaacta cctgatgacc cgcttctact aaagcctgta
     3121 atcccgcgac agcaaaatcg ggagtaccca tatatacgat acgtaatttt ttagccatta
     3181 ttcaaaatcg gggtaaagta aatattgttt acgtactttt ttaaatgttg atatatcatc
     3241 ttgccaactc aaacgaattt cttcttccga taatccgcct tttagttgca tcatcaactc
     3301 agcactacct gctaatttat taaagaaatt attttcaaga aaaaatttat ccttaggttc
     3361 aaattcctga taaattttaa gcaacaattg aatatcgatt tttccatgtt gtacctcatc
     3421 catactttta gcagaaaaat cgtatccata acatttggta tgcattaaaa aaggtgattt
     3481 tgcaccgggg ccgggcattg gggtaaattc aaatccggta ttttttaaat acggcgaacc
     3541 gataatttca aatggttttg cagtacctct acccacactc acatttgtac cttcaaaaaa
     3601 acataatgtt ggatacatat aaatggctcg catatttttc aaattcggcg atggtgcaat
     3661 aggcacttcg tacatttttt tatggtcgta atttttgcag ggaataactg ttaaatcgca
     3721 ttgaacacca ttcgctaacc atttttcacc gtttatcatt ttggcatatt caccaatggt
     3781 cataccatgc acaacaggta ttggatgcat gccgacaaac gaactatagg ctttatctaa
     3841 taaaggacca tccacataat aaccatttgg gttaggtcgg tccaaaacta taattggttt
     3901 gcctaattca gcacaagctt ccattacata atgtaacgaa ctgatatagg tataaaaccg
     3961 tgcaccaaca tcctgaatat caaaaacaac tacatctaca tcggcaaggt cttgttgcgt
     4021 tggtttcttt ttgttgccat ataaggataa taccggtaat cctgttttag tatcttttgc
     4081 atttagaatg gtagcacctg catcttcagt gcctcgaaat ccgtgttcag gtgcgaaaat
     4141 ggttgcgata ttaacgccgg aagcaatcag ggagtctacc aagtgtttat caccaattgt
     4201 gctggtttga tttaccacac aggcaatgta tttacctttt attaatggca acaacaactc
     4261 agtttgctga gcaccggtta ttatgtctgt atcataatta acctgtttat gcacctccgt
     4321 agggttagac acaggttgag cattatcaat tgcagaatca ttatttttac aggcaattga
     4381 aacagtaaac agagtaaaag ctagagctat tattgacata cgcatgtttc attcaaaact
     4441 aaggccaaag ttataaagta attagggatg tggttcgaat tttttatcac caaaaagata
     4501 attcataata aagaacggtc gttttcgcga ttgattattg ccatagcccg cgttgctgtt
     4561 gcactcagcc taagtgtaat gatcatctca acctcgttgg taaacggatt tagagctact
     4621 atttctgata aagtatatgg tttttgggga catattaata tcgcaaaaca aagtttacgt
     4681 aattccttcg atgatgaacc catttacagg gtgcctgaat ttgttgaaca ggtaggcaag
     4741 attgatggtg ttacttcagt gcaggtttat gcgcgaaaag ctgccatcat taaaaccaaa
     4801 acagatattg aaggtattat catgaagggc attggcagcg attttgactg gcaacaactg
     4861 aataaatata tcatcgaagg cgacccgatt gatttaacgc aaactgatgt gagtcgtggt
     4921 tcactaattt caaaatcgac tgcggacaga cttggatttg tggtgggtga tagtatttta
     4981 ttacatatta tcgaccaaaa agaaaatggc gattacgaac aaatgtatcg caaattacgt
     5041 gtaaatggca tttataatac cggactcgaa gaatttgata aattgtttgt gttggtcgac
     5101 atcagacata ttcagcggtt aaacaactgg tcgcaagatc aaattggtgg ttatgaagta
     5161 tttattgatg accctgaaaa aattgatgtt atagagccaa tagtggataa aaaagcagac
     5221 ccgttttggg atgtgcaaac cattcagcaa ataattccca gtgtgttcga ctggctgaac
     5281 ttgcaaaaag taaatgaatg gataattctt accttaatga ttttggtagc gctcattaat
     5341 atggtgacat cattattgat tttgatttta gaacgtacca atatgattgg tattttaaaa
     5401 gcattgggtg ccaacaattt ttctattcgt aaaatatttt tattaaatgc cgccaatatt
     5461 attttgaaag gcatgttatg gggaaatata atcggaattg ggttgtgttt attacaatta
     5521 caatttggtt taattaaact ccctgaagct tcttattatg taaaagtagc tcctgtacag
     5581 attaatgtaa tatgggtttt attactcaac gcaggaacgt ttgtaatatc tattttattc
     5641 ttattaatcc cttctttatt tgtggctaaa ataaggccta ttaaagccat cagatttaat
     5701 taattcactt attgttatta tccggagatg tttgtgttgg attgttgtac gggcaatgtg
     5761 tacatccgtt accgcaacaa tatccacgct ccagcaaata cttttcagta aataccaaaa
     5821 ggccatcctt attgatgtaa tagtcaatat tttcgattaa agcaagagac ggcatcagcg
     5881 atgtaattag aaataacgca tacccgtata gttgaatggt gtaatacctc gcaactgttc
     5941 tttaatgtct tcacttacat ctaatccttc aataaattcc tgcatggtag ctttggtgat
     6001 tgcctgtccg tttctggtca ggtttttcaa cgcttcataa ggctgaggat aattttcacg
     6061 acgtaaaata gtttgaattg cctctgcaat tacagcccag ttattatcta aatcgtcgtg
     6121 aatttttgcc tcgttaactt gtaatttttt caagccacgt gaaatcgatt taaatgccag
     6181 aatggtatgc gccattggca ctccgatatt acgcaacaca gtcgagtcgg ttaagtcgcg
     6241 ctgtaaacgt gaaattggca acttagcaga taaatgttca aacatggcat tagcgatgcc
     6301 caaattacct tctgcatttt cgaagtcgat tggatttact ttatgcggca tggctgaaga
     6361 gccaacttca tctttattga ttcgttgttt aaaataatcc atcgaaatat aagtccataa
     6421 gtccc
//
LOCUS       DAORKW010000168         6421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_423_length_6421_cov_4.561734, whole genome shotgun sequence.
ACCESSION   DAORKW010000168 DAORKW010000000
VERSION     DAORKW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_423_length_6421_cov_4.561734"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLB46_08020"
     CDS             complement(<1..602)
                     /locus_tag="PLB46_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76503.1"
                     /translation="MRIPTYIVVVLTLALLVSSQGCQQTANGTNDNKKQPSGMAAPVS
                     PYATDATWNNYWYGGKAELTSYYLKQSRYGEIHEGTVVNVFVTEDFSKSKQVKLDNPS
                     AAGADKLPILKLNQSYKFNTGIYPYSVMISAFQPIDINNYPHAVKITGSMQEWCGMAY
                     YQMNSGDNGFNIESRSYFESEGDKQLTIKTIVQEDELWNL"
     gene            complement(671..2122)
                     /locus_tag="PLB46_08025"
     CDS             complement(671..2122)
                     /locus_tag="PLB46_08025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF76504.1"
                     /translation="MPEIQSLYRKYGWFTIILLLFIVAKLPAMQLPYFWDELGVYSRA
                     GLYLHDNGLGLLPKNLPPELSRGHPLLFSFIHGCGYFIFGDGVVGGHITGLIIALFLL
                     LSIYKIAYYHFDQKTALLSVMLVIVQPVFFAQSILVLPEICLALFMLWAIHYWSIRQY
                     GWYALMSTAAILIKETAIIIPVVVITASIINWLVQRPRKNMGFQWKQLLVLTPFVIFG
                     LFLIIQRQQNGWYLFPLHGDNVDFKIKKIIEFGIDFMSFTFIEQGRIVLLVAGLIVTI
                     LILYKKLWHYHRFIVYLIMLFVGGILFSSLNFFMNRYLVFAIIPLIMVFAHLLITAVA
                     TYRWLYVFVPLVFFAGMISMYGYELYPVSDETMSMEAKFHYDEDMSYIYFLDAQIEAV
                     NFVNEKANNTTQFFVNFPMVFAFNEPRFGFPVSKTKTIIQVKTKQKGFEYAVISNPGS
                     YDFKLPGGDSLRLVKMIKNEVVEIPIYEPNFEW"
     gene            complement(2258..4486)
                     /gene="purL"
                     /locus_tag="PLB46_08030"
     CDS             complement(2258..4486)
                     /gene="purL"
                     /locus_tag="PLB46_08030"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HRF76505.1"
                     /translation="MSEQLATVEQARQLGLLAEEFEHIKQILGRTPNFTELSIFSVMW
                     SEHCSYKNSITLLKTLPRKGKKMLVEAGEENAGLIDIGDGLGCAFKIESHNHPSAIEP
                     YQGAATGVGGIHRDIFTMGARPIASLNSLRFGNINNKKTQHLMRGVVKGIGDYGNCFG
                     VPTVAGEVYFEDCYNTNILVNAMSVGIVKVGETVSAIAEGKGNPVYIVGSATGKDGIH
                     GASFASGDLHDESHEDLPSVQVGDPFQEKLLLEATLEAIKTGAIVGMQDMGAAGITCS
                     TSEMSAKGKHGMKIYLDKVPTRQKNMKGWEILLSESQERMLVVVKKGMEKDIEAVFDK
                     WDLHCEIIGEVIEGELLQYYMHGELIAEVPAESLVLGGGAPIYQRESKEPEYIKEIQS
                     FDANSIAVPADLKAVAKKLASNPNIANKRWITVQYDSMVGVKNTSTNLKSDASLIRII
                     GKNKGLAVTTDCNSRYVFSDPYVGAMIAVSEAARNIVCSGAEPAAITNCLNFGNPYNP
                     EVYWQFSNAIKGMGDACRKFDTPVTGGNVSFYNQSEEGPVYPTPTIGMVGVLEDFKNQ
                     MTLDFINSGNVIMLIGKATDDIACSEYLKDIHGITKSPAPHFDLNDEYALQQTIKELI
                     RNQLIYSAHDVSEGGLFTTLLESAMVNSLGFEIETDATIRKDAYLFGEGQSRVVVSTS
                     SEKAFVIENICKEFNVPVNVLGTVTDGKISIDGEQFGNTIDYKNLYDNAIGQYMAQEV
                     AV"
     gene            4743..>6421
                     /locus_tag="PLB46_08035"
     CDS             4743..>6421
                     /locus_tag="PLB46_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF76506.1"
                     /translation="MLGNNAEIIAQNLVTNPGFETYLHLPDSYGDWNFSQGWHNCSGD
                     GSPDYLHTSGISVANLPDCGLGQIYPYDGNAAMGFSTYRSDFEHREYISTELTSPLII
                     GETYIVSFWLTNGESDWYSGTGTNHIGIYFSHAMPNQAATNTINVTPQVDITDIVWLT
                     DWQYFEFTYTATDDFSQITIGNFFEDVNTETNIFYELADNPTRSEYIIDNIYVGLVPE
                     PETVLIDTTLCLGNSYFLPDGSEIFASTIDTTILSDTDGADSIIITNVTFISSIVVEQ
                     TVHICEATSYVLPDGNYAYSEGTYTSIISTAGGCDSTVVTTIMIATAPEITVLAPVYI
                     CADAEAISLDAFPSGGVFEGPGIIENNFNPQLTDGEGTYTLSYTLNMNECSFIQLFTI
                     TVIENLAMAGTDQTIQFGDRFILHGTTAGEIKWSPDNDLSCNTCDSPSGLINQTTTFL
                     LESINEYGCYAADEVTFTVLHEGPDSVFVPNTFTPNGDGINDYFIPLGLAINKIVHFQ
                     VYNRWGELMYSEENITPESTNQGWDGKFNGTEVQEGVYIYTAEIEYLSGIIV"
BASE COUNT         2031 a         1367 c         1024 g         1999 t
ORIGIN      
        1 atgagattcc acaattcatc ctcttgcact attgttttga ttgtaagttg tttgtcacct
       61 tccgattcga aataacttct ggattctata ttaaagccat tatcaccgga attcatttgg
      121 taataagcca taccacacca ttcttgcata ctacccgtaa ttttaactgc atgcggataa
      181 ttattaatat caattggttg aaaagcacta atcattactg aataaggata aataccggta
      241 ttaaatttat acgactgatt taattttaat atgggcaatt tatctgcacc tgctgcagac
      301 ggattatcca actttacttg tttcgattta gaaaaatctt cagtaacaaa aacatttaca
      361 acggtgcctt catgaatttc tccgtaacga ctttgtttaa ggtaataact agttaattca
      421 gctttaccac cgtaccaata attattccag gttgcatctg tggcataagg agataccggt
      481 gctgccattc cggaaggttg ttttttatta tcatttgtgc cgttagcagt ttgctgacaa
      541 ccctgactac tcaccaaaag tgcgagtgtt aatacaacaa caatataagt aggtatacgc
      601 ataacaaata atttatatta accttaatta atggtgaaat tcaaacatga atagccgata
      661 aaaagttcca ttaccactca aaattcggtt catagatggg aatttccact acctcatttt
      721 taatcatttt caccagtcgc aaactatcgc ccccgggcaa cttaaaatcg tacgaaccgg
      781 gattacttat aacagcatat tcaaaaccct tttgttttgt cttcacttgg atgattgttt
      841 tggtttttga aaccggaaat ccaaatcgag gttcattaaa tgcaaacacc attgggaaat
      901 tgacaaaaaa ctgtgtcgtg ttgtttgctt tttcatttac aaaattcaca gcctcaatct
      961 gcgcatccag aaaatagata taactcatat cttcatcata gtgaaatttt gcttccatcg
     1021 acatagtctc atcactcacc ggataaagct catatccata catggaaatc attccggcaa
     1081 aaaacaccaa tggaacaaat acatataacc atctgtaagt tgctaccgca gtaataagca
     1141 aatgtgcaaa taccataatc aaaggaataa tggcaaacac taaatagcgg ttcataaaaa
     1201 agttgagcga gctgaacaaa atgcccccaa caaataacat aattaagtaa acaataaatc
     1261 tatgataatg ccataatttt ttgtataata tcaggattgt aacaattaat cctgcaacta
     1321 ataacactat tcgtccctgt tcaataaatg taaaggacat aaaatcaata ccaaattcaa
     1381 taatcttttt tattttaaag tccacattat cgccgtgtaa tggaaataaa taccagccat
     1441 tttgttgacg ctgaataatt aaaaacaatc caaaaattac aaacggtgtc aacactaata
     1501 actgcttcca ttgaaagccc atgttttttc ttggccgttg aaccaaccaa tttataatgg
     1561 aggcagtaat tacaaccacc ggaattataa tcgccgtttc ttttatcaaa atggcagcag
     1621 tggacatcag tgcataccat ccatattgtc ggatcgacca ataatgaata gcccataaca
     1681 taaacagtgc aaggcaaatt tccggcaaca ctaaaatcga ttgtgcaaaa aataccggtt
     1741 gcacaataac cagcataaca gacaacaaag ctgttttttg atcaaaatga taataagcaa
     1801 ttttataaat actcaacaat aaaaacaggg cgataattaa tcctgttata tgaccaccta
     1861 ctaccccatc gccaaaaata aaataaccgc aaccgtgaat aaatgaaaat aataatggat
     1921 gcccgcgtga aagttccggt ggcaaatttt taggcaacaa ccctaaccca ttgtcatgca
     1981 aatacaaacc tgcgcgtgaa tacacaccta attcgtccca aaagtatggc agctgcatgg
     2041 caggtaattt agccacaata aaaagcagca aaataatggt aaaccatccg tatttgcgat
     2101 ataaagattg aatttccggc aattgaaaaa ttgagtttaa acctttatca ggctttcaaa
     2161 aataagggta ttttaattgc caaacaactt gctgctgtaa gtataaaaaa aaaggcagcc
     2221 tgttcagact gccctattat aaagtgtatg atttgtatta aacggcaact tcctgagcca
     2281 tatattgccc aatggcattg tcgtataaat ttttataatc aatggtatta ccaaactgct
     2341 cgccgtcaat actaattttt ccatctgtaa cagttcccaa tacgttaacc ggaacattaa
     2401 attctttaca aatattttca ataacgaaag ctttttcgct tgatgttgaa acaaccaccc
     2461 tgctctgacc ttcgccaaac aaatacgcat ctttacgaat agtggcatct gtttcaattt
     2521 caaatccaag gctattcacc atcgcacttt ctaacagtgt tgtaaataat ccaccttcgc
     2581 tcacatcatg cgcagaataa attaattgat tgcgaattaa ttctttaata gtctgttgca
     2641 atgcatattc atcatttaaa tcaaaatgtg gcgccggcga tttagtaatg ccatgaatat
     2701 ctttcaaata ttcgctacag gcaatatcgt cggttgcttt accaatcagc attattacat
     2761 tacctgaatt aataaaatcc aatgtcattt gatttttaaa atcttccaac acacctacca
     2821 tgcctattgt tggtgttgga taaacaggcc cttcttcact ttgattataa aatgaaacgt
     2881 ttccgcctgt taccggtgta tcaaattttc tgcacgcatc gcccatacct ttaatagcat
     2941 tgctaaactg ccaataaact tcagggttat aaggattacc aaaatttaaa caattggtaa
     3001 tagcagcagg ttctgcacca ctgcacacaa tatttctcgc agcttcactc accgcaatca
     3061 ttgcccctac ataagggtca gaaaatacat aacgcgaatt acaatctgta gttactgcta
     3121 agcctttatt tttaccaata attctaatta gtgatgcgtc actttttaaa ttagtagatg
     3181 tattttttac cccaaccata gaatcgtatt gaacggtaat ccatctttta ttggcaatat
     3241 taggattcga tgctaatttt tttgccactg cttttaaatc agctggtact gcaattgaat
     3301 tggcatcaaa cgattgtatt tctttaatat attccggctc ttttgattca cgctgataaa
     3361 ttggtgcacc accccctaat accaaacttt cggcaggcac ttcagcaatt aattcgccgt
     3421 gcatataata ttgtaacaat tcaccttcaa tcacttcacc tataatctcg caatgtaaat
     3481 cccatttatc aaaaactgct tctatatctt tctccattcc ttttttcact actacaagca
     3541 tacgttcctg actttcactc aataatattt cccaaccttt catatttttc tgacgggtag
     3601 gtactttatc gagataaatt ttcattccgt gtttaccttt tgcactcatt tcagatgttg
     3661 aacaggtaat acccgcagcg cccatatcct gcataccaac aatggctccg gttttaatgg
     3721 cttctaatgt agcttccaat aataattttt cctgaaatgg atcaccaacc tgcaccgatg
     3781 gtaaatcttc atgactttca tcatgtaaat cgccactggc aaaactcgca ccatgaatgc
     3841 catctttacc agttgcacta ccaacaatgt aaaccggatt cccttttcct tcagcaattg
     3901 ccgaaaccgt ttcaccaact ttcacaatac caacactcat ggcattaact aatatgttgg
     3961 tgttataaca atcttcaaaa tatacctcac cggcaacagt tggcacgcca aaacaattgc
     4021 cataatcgcc aatacctttt accacaccgc gcattaaatg ctgcgttttt ttattgttga
     4081 tatttccaaa acgcaatgag tttaatgaag caataggcct cgcacccatc gtaaatatgt
     4141 cgcgatgtat accaccaact ccagttgcag ctccttgata aggttcaata gccgatgggt
     4201 ggttgtgact ttcaatctta aaagcgcagc ccaaaccatc accaatatca ataagtccgg
     4261 cattttcttc tccggcttct accagcattt ttttgccttt tcttggtaag gtttttaaga
     4321 gagtaatgga atttttgtaa ctgcaatgtt cgctccacat tacgctgaaa atactgagtt
     4381 cagtgaaatt tggtgtgcga cctagtattt gtttgatgtg ttcaaattct tctgcaagca
     4441 gacctagttg cctggcctgt tctactgtag caagttgttc ggacatattt tttgattgag
     4501 gcacaaaatt aatcccgaaa acctaaactc ttgccgattt atgcagattt tatgttgaca
     4561 attttcagct ccctaagcca aaccaccatt tcgccctcaa tatcacgcat tccgctaatt
     4621 accgcttttc acctcaataa tgcagcccta atttggtctc cgaaaacaca ataataccat
     4681 tcatgcatat taacaatttt tccaaatcac ccaattttat ctggttttgc ttcctgcttt
     4741 ttatgttggg aaacaatgca gagataattg ctcaaaatct ggtaaccaac cccggtttcg
     4801 agacctattt acaccttccg gatagttatg gcgattggaa ttttagccaa ggatggcaca
     4861 actgctccgg cgatggcagt cccgattatt tgcacacctc tggtataagc gttgccaact
     4921 taccggattg cgggttaggc caaatttacc cttacgacgg aaatgccgcc atgggttttt
     4981 ctacctatcg cagcgatttt gaacatagag agtatatttc caccgaacta acctcaccat
     5041 taattatagg cgaaacatac atcgtctcat tttggttaac caatggtgaa tcggattggt
     5101 attccggaac cggcaccaat catatcggga tatattttag tcacgccatg cccaaccaag
     5161 ctgcaaccaa taccatcaat gttacccctc aagttgatat taccgatatt gtttggttaa
     5221 ccgattggca atattttgaa tttacttata cggctaccga cgatttttcg caaatcacta
     5281 ttggtaattt ttttgaagat gtaaataccg aaacaaatat attttatgaa ctcgccgata
     5341 atccaacccg cagtgaatat attatagata atatttacgt tggtcttgtt cccgaacctg
     5401 aaaccgtatt aattgatacc acactttgtt taggcaacag ctatttttta cccgatggca
     5461 gtgaaatttt tgcctccacc atcgacacaa ctatactatc agatactgat ggtgcagatt
     5521 ccataattat taccaatgta acttttattt catcaatagt agtggagcag actgtccata
     5581 tttgcgaagc aacaagttat gttttacccg atggtaatta tgcgtatagc gaaggaacgt
     5641 atacctctat aatcagcact gccggtggtt gtgattctac tgttgttaca acaattatga
     5701 ttgccacagc gcctgaaata actgtgttgg cacctgttta catttgtgct gatgcggagg
     5761 caatttcgtt ggatgcattt ccatccggag gtgttttcga aggtcccggt attatcgaaa
     5821 ataattttaa tccccaatta actgatggcg aaggcactta tacattgagt tatactttga
     5881 atatgaatga atgttcattc atacaattat ttactattac cgttatagaa aacttagcca
     5941 tggcaggcac cgatcaaacc atacaatttg gcgacagatt tattttacat ggcaccacag
     6001 ccggagaaat aaaatggtcg ccggataatg atttgagttg taatacttgt gattctccaa
     6061 gtggattaat taaccaaacc accacatttt tattggagtc gataaatgaa tatggatgtt
     6121 atgctgcaga tgaagtgacc tttactgttt tacacgaagg cccggacagt gtttttgtcc
     6181 cgaatacttt tacccctaac ggagatggta ttaacgatta ctttatacca ctaggcttag
     6241 ccattaacaa aatcgttcat tttcaggtgt ataatcgctg gggagagtta atgtacagcg
     6301 aagaaaacat cacacctgag tcaacaaatc aaggctggga tggcaagttt aatggtacag
     6361 aagtacaaga aggtgtgtat atttatactg ctgaaattga gtatttaagt gggataattg
     6421 t
//
LOCUS       DAORKW010000169         6410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_425_length_6410_cov_5.119906, whole genome shotgun sequence.
ACCESSION   DAORKW010000169 DAORKW010000000
VERSION     DAORKW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_425_length_6410_cov_5.119906"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PLB46_08040"
     CDS             complement(<1..314)
                     /locus_tag="PLB46_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HRF76507.1"
                     /translation="MEIHFSKYNFKITANGEQQMIFDIIRKKNVVLTPEEWVRQHIVH
                     YLINDYGFPKSLISLEKQLVLNELSKRTDIVLYNQTASPCMIVECKAPEVKITQKTFD
                     QIA"
     gene            350..1360
                     /gene="holA"
                     /locus_tag="PLB46_08045"
     CDS             350..1360
                     /gene="holA"
                     /locus_tag="PLB46_08045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRF76508.1"
                     /translation="MDYRDLVSDIKARKFKPVYLLHGEESYYIDKISEFMEDNILTPE
                     QKDFNCSIFYGKDSTPTQVMDAAMRFPMFADFNLVIVREAQMMKTRGSELDKLETYVE
                     RASPTTILVICYKDGKYDARKKLFKQIKDRGLVFESEALKESEVPIFIEAYLKRKKVT
                     AESKAIRVLIDYLGTDLAKMTNELDKLCINLPANGQITVEQIEKNIGISKDYNVYELQ
                     SALLMKNTEQVFTIVDYINDNQKANPFVLTIASIHAAYQKLYHLILGGDVGDFDMFKI
                     YGIHSSQKNEFRSARKHYSRQRVEEIFELILEYDLRSKGVENGETEHGALLKELAYKL
                     IS"
     gene            complement(1349..2323)
                     /locus_tag="PLB46_08050"
     CDS             complement(1349..2323)
                     /locus_tag="PLB46_08050"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRF76509.1"
                     /translation="MRHKSLHLRWDMQVLRDLNQLPDLSGSVITIGTFDGVHSGHYEI
                     IQKITEAARAIGGKSVIITFHPHPRHIITPESPVYYLTTLEEKIAKLSVCGVDVVVVV
                     PFSREFSEISAESYAEHFLIEKFKPAIIVFGYDHKFGKDRSGDIHLLKSIATKYTIRV
                     EEIPAHVIDTVTVSSSKIRKYLKEGNIVEANELLGYPYEITGPVIKGDQIGRTLGFPT
                     ANIYVEDSNKLIPSDGVYLVKVFIKNNPTKYFGLLNIGTRPTFNKTEKRIEVNILQFA
                     DTIYGETITVQFLHFLRADKKFDSTTSLIAAMEGDKQKAEAIISNLTN"
     gene            2336..3784
                     /locus_tag="PLB46_08055"
     CDS             2336..3784
                     /locus_tag="PLB46_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76510.1"
                     /translation="MVNRFVYKPNNQDIYFFLGLLFTIYLTIRAIVIPITIDEAGTYY
                     NYMPRSVTDILLFNGDPSPNNHILNTLSMKLFTALFGTTTFVVRLPNVLGFILFYTAA
                     VRLFTLMFKNRTLVVVALLVLLCNPYLLDFFAVARGYGLSIAFMFWSLYQFFQYYNTK
                     KNQFAVTTLIAAALAVLANFTLLHFYILIFFVIVVIAFAINFNKWKHLLIYVSITLIL
                     AAICYTPLTKMMQTNQLVYWETAGFYTSTLVPLVEQMRYGELYFGCKQAQPFANIILL
                     LFGLMIFVAVFYFFKKPNGKQLPFGSWILILLSGNIGLILIQFYLWHVPFLNGRGALF
                     LYPIFVLALLALLQELMTNSFKFPKYILLILAGMGIMHIARTANITSIREWWFDAHTK
                     EVLAWINQQHPGKENVSLNSSWIFNNSLHFHKDVQQFYNLDIAPYHQDLWPDSNYLYY
                     YCQREEVSKFDSAYNEVKSFNYGSFVIMKKSN"
     gene            complement(3781..4473)
                     /gene="truB"
                     /locus_tag="PLB46_08060"
     CDS             complement(3781..4473)
                     /gene="truB"
                     /locus_tag="PLB46_08060"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRF76511.1"
                     /translation="MFTAEELVAGKIILIDKPLHWTSHDVVAKVRIPIKIFCRDRKMK
                     IGHAGTLDPLATGLLILLTGKATKQTESLQGLEKEYTGTITLGATTPSYDAETPVDAT
                     FPIQHITPELIHETAKSFLGVQEQIPPIYSSVQVDGIRAYNYAREGETVKLKARTVEI
                     FRFEITAIRMPEIDFIVHCSKGTYIRSLTHDFGKAIGSGGYLTALRRTKVGDFDVQDA
                     LSLENMLAAVKI"
     gene            complement(4475..5278)
                     /locus_tag="PLB46_08065"
     CDS             complement(4475..5278)
                     /locus_tag="PLB46_08065"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRF76512.1"
                     /translation="MTILEAVVLAIVEGITEFLPVSSTGHMIIASSLMGISHDEFTKS
                     FTVAIQFGAILSVVVLYWKRFFKSINFYFKLFVGFIPAAILGFLLGDYIDAMLENVTV
                     VAIALIAGGVVLLFVDRLFGADITGDVSTTDHIGYKNAFVIGVFQCIAMIPGVSRSAA
                     TIIGGLTQKLTRKVAAEFSFFLAVPTMFAATGYKLYTLYSDGPGFTSDDYMPLIIGNV
                     VAFIIAIFAMRSFVNYLTKHGFKIFGYYRIIVGTIILVLLALGVDLQIV"
     gene            complement(5288..5572)
                     /locus_tag="PLB46_08070"
     CDS             complement(5288..5572)
                     /locus_tag="PLB46_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HRF76513.1"
                     /translation="MENQQPAKKDNPKVTSAPTTDGKMVLGKENYILIVLGLVVIIIG
                     FILMSGGKSPDPNVFNPDEVYSTRRITVAPIVVLLGFAIEIYAVFHRSKK"
     gene            complement(5594..>6410)
                     /locus_tag="PLB46_08075"
     CDS             complement(5594..>6410)
                     /locus_tag="PLB46_08075"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRF76514.1"
                     /translation="TSLALLMLGLMSILFISFKYEEANIKEDMVISAYLKEGLTAADV
                     DKLLKKIEGEASVKATKFISKDDAMKIFEEKFNENPSEILGDVNPLPASVDVTLAASA
                     VNVDSIKLFREKLQLYPEITHTRANEELISSVDSDLKIAGFIIAGFSILFLIISIAII
                     DKTIRLSMYSNRFLVRSMQLVGATRSFITSPYVRRSIINGLVSAGIAMLLLLFGVLYV
                     HNNFNYWDFSNTTIKRGFIFTFLMLIGVGVLITWFSTRNSVHKYIRMKLDELY"
BASE COUNT         2039 a         1188 c         1130 g         2053 t
ORIGIN      
        1 gcaatctgat caaatgtttt ttgtgtaatt tttacttcag gggctttaca ctcaactatc
       61 atgcaagggc tagccgtttg attataaaga acaatatcgg tgcgcttcga caactcattt
      121 aaaacgagtt gcttttcaag actaatgagc gatttaggaa aaccatagtc gttaattaaa
      181 taatgcacaa tatgctgtct cacccattct tccggtgtta gcaccacatt ttttttgcgg
      241 ataatatcaa aaatcatttg ctgctctcca ttggcagtta ttttgaaatt atactttgaa
      301 aagtggattt ccatcgcgcc gtaaatttac aattaattac atttgcacta tggattatcg
      361 cgatttggtg agtgatataa aggctcgaaa gttcaaaccc gtttacctgc tacacgggga
      421 agagtcctat tatatcgaca agattagtga gtttatggaa gataatatcc taacccccga
      481 acaaaaagat tttaactgct cgatatttta tggcaaagat tctaccccaa ctcaggttat
      541 ggatgctgcc atgcgtttcc ctatgtttgc cgattttaat ctggttatag tgcgcgaagc
      601 ccaaatgatg aaaacaagag gcagtgaact cgataagtta gaaacctacg tagaaagagc
      661 ctcaccaaca actatcttgg ttatttgtta taaagatggg aaatacgacg ccagaaaaaa
      721 attatttaaa cagattaaag acagaggttt agtttttgaa tcggaagcct taaaagaatc
      781 cgaagtgcct atttttatcg aagcttatct gaaacgaaaa aaagttacgg cagaaagtaa
      841 agctattcgg gttttaatag attacctggg aaccgatttg gcaaaaatga ccaatgagtt
      901 ggataagtta tgtattaatt tacccgccaa cggtcaaatt actgtagagc aaattgaaaa
      961 aaatattggc attagtaaag attataatgt ttatgaatta caaagtgcct tgttaatgaa
     1021 aaatacggag caggtattta ccatagttga ttatatcaac gataatcaga aggcaaatcc
     1081 atttgtgctc actatagcat ccattcatgc ggcttatcaa aaattgtatc accttatttt
     1141 aggtggtgat gttggcgatt ttgatatgtt taaaatttat ggtattcact cttctcaaaa
     1201 aaatgaattc cggagtgcgc gcaaacatta tagccgccag cgtgttgagg aaatttttga
     1261 attaatactg gaatatgatt tacgttcaaa aggcgtcgaa aatggcgaaa ctgaacatgg
     1321 ggctttactt aaagaattag cgtataaatt aattagttaa gttgctgata atggcttcag
     1381 ctttttgttt atccccttcc atggccgcta tcagtgaagt tgtggaatca aattttttgt
     1441 ctgctcgtaa aaagtgtaaa aactgcaccg taattgtttc tccataaatg gtatcagcaa
     1501 attgcagaat atttacttcg atgcgttttt ctgttttatt aaatgttggt cgggtaccta
     1561 tatttaataa gccaaaatac ttagttgggt tatttttgat gaacactttt acgaggtaaa
     1621 caccatcgga aggaattaat ttattgctat cctccacata aatattggca gttgggaatc
     1681 caagagtacg gccaatttga tcgcccttaa ttacaggccc ggtaatttca tacggataac
     1741 ctaataattc gttggcttca acaatattac cttcttttaa atatttcctg atttttgaag
     1801 aactaaccgt tacggtatca atcacatggg caggaatttc ttccacacga atagtatact
     1861 tcgtagctat cgatttgagt aaatgaatat caccggaacg atccttccca aatttatgat
     1921 cataaccaaa tacgattatt gccggcttaa atttttcaat taaaaaatgt tcagcgtacg
     1981 attctgcact aatttctgaa aactctctgg aaaatggaac aactacaact acatcaacac
     2041 cacaaacact caattttgca attttttctt ccaatgttgt taaataataa accggcgatt
     2101 caggtgtaat aatatgccgt ggatgtggat gaaaagtgat gataacactt ttacctccaa
     2161 ttgcccgcgc agcctcagtt attttttgga tgatttcata atgcccgcta tgcacaccat
     2221 caaaggtgcc aatcgtaatt acagaacctg ataagtcagg cagttgattt aaatcgcgta
     2281 aaacttgcat atcccaccgc aaatgtaaag atttgtggcg cattagtata taaccatggt
     2341 gaacagattt gtgtataaac ccaacaatca ggatatttat ttcttcctgg gtctgctgtt
     2401 taccatttat cttacaattc gtgctatcgt tattcctatt actattgatg aagcaggcac
     2461 ttattataat tatatgccaa ggtctgtaac cgatattttg ttatttaacg gcgacccttc
     2521 cccaaataat cacatactga atacattaag tatgaaatta ttcaccgctc tttttggaac
     2581 taccaccttt gttgttcgtt tgccgaatgt attgggattt atactttttt atactgccgc
     2641 ggttcgttta tttaccctta tgtttaaaaa ccggacattg gtagttgttg cattattagt
     2701 gctactttgt aatccttatc tgctcgattt ttttgcagtg gcaagaggtt atgggctgtc
     2761 tattgctttt atgttttggt ccttgtatca attttttcaa tattataaca ctaaaaaaaa
     2821 tcaatttgca gtaacaactt taatagcagc agcattagca gtgttggcca atttcacact
     2881 cctgcatttt tatatattga ttttctttgt gatagtggtt atagcatttg ccatcaactt
     2941 taataaatgg aagcatctgc tcatttacgt gagtataacg cttatacttg cagctatttg
     3001 ttatacacca ttaacaaaaa tgatgcaaac aaaccagctg gtatattggg aaactgccgg
     3061 gttttataca tcaactttag ttccattagt tgagcaaatg cgatatggtg aattgtactt
     3121 tggatgtaaa caggcgcaac cttttgctaa cattatttta ttattatttg ggttaatgat
     3181 ttttgttgca gtattttatt tcttcaaaaa acctaacgga aaacaattgc catttggaag
     3241 ctggatacta atattattaa gtggaaatat tgggcttatt cttatacagt tttatttatg
     3301 gcatgttccg tttttaaatg ggagaggagc attattttta tacccaatat ttgttcttgc
     3361 ccttcttgct ttactgcagg agctaatgac caactcattt aaatttccga aatatatatt
     3421 gctgatttta gcagggatgg gtatcatgca tattgcccga acagcaaata ttaccagtat
     3481 tagagaatgg tggttcgatg cacatacaaa agaagtatta gcatggataa atcagcagca
     3541 tcccggtaaa gaaaatgttt cgttaaatag ttcctggatt tttaataatt cattacactt
     3601 tcataaagat gtgcaacaat tttacaattt agatatcgca ccttatcatc aggacttgtg
     3661 gccggattcc aattatttgt attattattg tcaaagagaa gaagtatcga aatttgatag
     3721 cgcctacaat gaagtaaaaa gttttaacta cggttcattt gtaataatga aaaaatcgaa
     3781 ttaaatttta actgctgcca gcatattttc taaactcaaa gcatcctgaa catcaaaatc
     3841 cccaactttt gttctgcgca aggctgttaa atacccacca ctgccaattg cttttccaaa
     3901 atcatgcgta agtgaacgta tgtaagtgcc tttactacaa tgcacaataa aatcaatttc
     3961 aggcattcgg atcgcggtta tctcgaagcg aaatatttcc acagtacgtg cttttaattt
     4021 cactgtctcg ccttctcttg cataattata tgcacgtatt ccatccacct gaacggaaga
     4081 atatattggt ggaatttgtt cctgtactcc taaaaaactt tttgctgttt cgtgtattaa
     4141 ttcaggcgta atatgttgaa taggaaaggt ggcgtctacc ggtgtttcag catcgtaact
     4201 gggtgttgtg gcaccgagtg ttatggttcc tgtatattct ttttctaaac cttggagcga
     4261 ctcagtttgt ttagttgctt ttcctgtaag taatatgagt aaacctgtgg caagtggatc
     4321 taaagttccg gcatgtccga ttttcatttt gcggtcgcga caaaatattt taatgggtat
     4381 gcgcactttc gccaccacgt cgtgactagt ccaatgtaat ggtttgtcga tgagaatgat
     4441 tttacctgct actaattctt ctgccgtaaa catattacac aatctgaagg tcaacaccta
     4501 atgccaataa tactaagatg atagtgccaa ctataatgcg gtaatagcca aaaattttaa
     4561 aaccatgttt tgtcaggtaa ttaacaaaac tgcgcatagc aaaaatggca ataataaaag
     4621 ctacaacatt gccaattatt aatggcatgt aatcatcgct cgtaaatccc gggccatcgc
     4681 tatacaacgt atataattta taaccggttg cggcaaacat agtgggaaca gcaagaaaaa
     4741 atgaaaattc tgcggctact ttgcgcgtga gtttttgtgt taaaccgcca atgatggtag
     4801 cagcagaacg tgatacaccc ggtatcatgg caatacattg aaatacgcca attacaaatg
     4861 cgtttttata accaatatga tcagtagtgc ttacatcgcc ggtaatatct gcaccaaaca
     4921 atctgtcaac aaataataac acaacaccgc ctgcaataag tgcaatagca actacagtaa
     4981 cattttctaa catggcatca atgtagtcgc ctaataaaaa tcctaaaatg gcagcaggaa
     5041 taaatcccac aaataattta aaatagaaat taatactttt aaaaaagcgt ttccagtata
     5101 aaactaccac cgataatatt gcaccaaatt gaatggcaac cgtaaacgac ttggtaaatt
     5161 catcgtgtga aatgcccatt aaagaagagg caataatcat atgtccggta gaggacacgg
     5221 gtaaaaattc agttattcct tccactatgg caagaacaac cgcttcgagt atggtcatgc
     5281 tggataatta tttttttgaa cgatgaaata cagcgtagat ttctatcgca aaacctaaaa
     5341 gcactacaat aggtgcaacg gtaatacgac gtgtgctgta tacctcatcc ggattgaaaa
     5401 cattagggtc aggactttta ccgccgctca ttaatatgaa acctataatg atgacaacta
     5461 aacccaatac aatgagtata tagttttctt tgcctaatac cattttacca tcggtagttg
     5521 gggcagatgt aacttttggg ttatcctttt tagcaggttg ttgattttcc attatagcca
     5581 aatatttaaa aatttaatac agttcatcca gtttcatacg gatgtattta tgcactgagt
     5641 ttcttgtgct gaaccaggta atgagcaccc ccacaccaat cagcattaaa aatgtaaaaa
     5701 taaaacctct tttgatagtt gtgttactaa aatcccaata attaaaatta ttatgcacat
     5761 atagcacccc aaaaagcagc aaaagcatgg caattccggc tgaaacgagc ccatttatga
     5821 tacttcggcg aacataagga ctagtgatga atgaccttgt agcgcccacc agctgcatac
     5881 ttctgaccaa aaaacggtta gaatacatgc tcaatcgaat ggttttgtcg ataatcgcaa
     5941 tagaaatgat taaaaacagg atagaaaacc cggctattat aaatcctgca atcttcaaat
     6001 cgctatctac agagctgata agctcctcat ttgctctggt atgggtgatt tccgggtata
     6061 attgcaactt ttcacgaaac agtttaatac tgtctacatt tactgctgaa gcggctaagg
     6121 tcacatcaac agaggctgga agcgggttta catcacccaa aatttcggaa gggttctcat
     6181 tgaacttctc ttcgaaaatt ttcatggcat catccttaga gataaatttg gtcgctttta
     6241 ccgatgcctc accttcaatt ttctttaaca gtttatctac gtctgcagca gttaatcctt
     6301 ccttcaaata agccgaaata accatatctt ctttaatatt ggcctcttca tatttaaatg
     6361 aaataaacaa aatactcatc aaccccaaca tcagcaatgc caatgaggta
//
LOCUS       DAORKW010000170         6372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_431_length_6372_cov_3.823650, whole genome shotgun sequence.
ACCESSION   DAORKW010000170 DAORKW010000000
VERSION     DAORKW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_431_length_6372_cov_3.823650"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /gene="rpsG"
                     /locus_tag="PLB46_08080"
     CDS             <1..324
                     /gene="rpsG"
                     /locus_tag="PLB46_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRF76515.1"
                     /translation="VEKQTGENGYEVWKKALSNVTPGVEVKSRRIGGANFQIPTEIRP
                     DRKISLSMKWLIMFARKRSGKSMAEKLAAEVVAAAKGEGATIKKKEDVHKMAEANKAF
                     AHFKS"
     gene            347..2452
                     /gene="fusA"
                     /locus_tag="PLB46_08085"
     CDS             347..2452
                     /gene="fusA"
                     /locus_tag="PLB46_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRF76516.1"
                     /translation="MARDLKYTRNIGIAAHIDAGKTTTTERILFYTGITHKIGEVHDG
                     AATMDWMEQEKERGITITSAATTTHWKYRNEEYKVNIIDTPGHVDFTVEVERSLRVLD
                     GVVALFCAVGGVEPQSETVWRQANRYKVPRLGFVNKMDRSGADFLEVVKQVKEILGAN
                     PVPLVLPIGAEETFKGIVDLITNKAIIWDDETQGMTYKEVPIPDDMKDLVVEYRNHLV
                     EAVAEYDDALLEKFFDNPDSITEEELITAIRKATIDIAIIPMLCGSSFKNKGVQFMLD
                     AVVRYLPSPLDIDAVKGTNPDTEEELSRQPDSKEPFTALAFKIMTDPFVGRLAFFRVY
                     AGKLDAGSYVLNVRTGKKERISRILQMHANKQNPLDTIEAGDIGAAVGFKDIRTGDTL
                     CDENNPIALESIKFPDPVIGLAIEPKTQADVDKLGMALAKLAEEDPTFKVNFDEETGQ
                     TVVRGMGELHLEIIVDRLKREFKIEVNQGAPEVAYKESFTKTVKHREVYKKQTGGRGK
                     FADIQFEIGPADEGVVGLQFVNDIFGGAIPREYVPSVEKGFKEAMSTGVLAGYTMDSL
                     KVRLFDGSFHAVDSDAFSFEMAAKIAFRDAGKNAGPQLLEPIMKLEVITPEDHMGDVI
                     GDLNRRRGMIEGFDNKNNATVIKAKVPLSEMFGYVTQLRTITSGRATSILEFSHFAPA
                     PASVSEEVIAKSKGKVTVN"
     gene            2474..2779
                     /gene="rpsJ"
                     /locus_tag="PLB46_08090"
     CDS             2474..2779
                     /gene="rpsJ"
                     /locus_tag="PLB46_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRF76517.1"
                     /translation="MTYKIRMKLKSFDHTLVDKTAEKIVKTVRNTGAVVTGPIPLPTQ
                     KKIYTVLRSPHVNKKAREQFQMCTYKRLLDIYSATNKTVDALQKLELPSGVEVEIKI"
     gene            2825..>2926
                     /locus_tag="PLB46_08095"
     CDS             2825..>2926
                     /locus_tag="PLB46_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRF76518.1"
                     /translation="MTHFYGEEGKLISCTVIEAGPCVVVQKKTDDTDG"
     assembly_gap    2927..2936
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2937..3427
                     /gene="rplC"
                     /locus_tag="PLB46_08100"
     CDS             <2937..3427
                     /gene="rplC"
                     /locus_tag="PLB46_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRF76519.1"
                     /translation="GYNAVQLGFEELKEVRTNKAQTGHFKKSGAKPMRKIKEFRNFDL
                     DKNVGDNITVDIFEEGEKVSVVSTSKGKGFQGVVKRHNFSGVGMRTHGQHDRHRAPGS
                     IGQSSYPARVFKGLRMAGRMGNETVKQRNLKVAKIISDKNLILINGSVPGSKGSYVII
                     EK"
     gene            3429..4058
                     /gene="rplD"
                     /locus_tag="PLB46_08105"
     CDS             3429..4058
                     /gene="rplD"
                     /locus_tag="PLB46_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRF76520.1"
                     /translation="MKVEIVKISGEKTGKQVELNEEIFGVEPNDHAIYLSVKQYLANQ
                     RQGTHKSKEKGEAAGSTRKLHRQKGTGGSRKGSIKNPLFHGGGTIFGPRPRDYSFKLN
                     RKVKELARRSALSYKAKQNNIVVVEDFTLDTPKTKTFAGIMKNIGVSDKKTLFVLGDN
                     QNIRLSLRNLPKTGASKASDLNTYDIMNAQCIVLSESTVNLINQMAENN"
     gene            4072..4368
                     /gene="rplW"
                     /locus_tag="PLB46_08110"
     CDS             4072..4368
                     /gene="rplW"
                     /locus_tag="PLB46_08110"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRF76521.1"
                     /translation="MAKRILIKPLITEKTTKLSEANGTYGFVVGMDANKIEIKNAVEK
                     NYGVTVAAVKTITDNGKFGWKRTPFGISQGTKGRMKKAYVTLKKGETIDFFSTI"
     gene            4377..5201
                     /gene="rplB"
                     /locus_tag="PLB46_08115"
     CDS             4377..5201
                     /gene="rplB"
                     /locus_tag="PLB46_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRF76522.1"
                     /translation="MALKKYNPVTPGTRFRIGNAYAEVTTDTPEKSLTAPFKKTGGRN
                     SSGALTMRYIGGGHKKKYRIIDFKRDKHNVPATVKTVEYDPNRTAFIALVNYADGEKR
                     YILAPNGIQVGQTIEAGDNVAPELGNALMLRNIPLGTIIHNIEMQPGKGGMIARSAGS
                     WAQLSAKEGKYVIVRMPSGETRMILDTCYATVGTVSNPDHNLQQLGKAGRNRWLGVRP
                     RNRGVAMNPVDHPMGGGEGRSSGGHPRSRNGKYSKGMKTRHPKKTTSKFIISRRKK"
     gene            5212..5475
                     /gene="rpsS"
                     /locus_tag="PLB46_08120"
     CDS             5212..5475
                     /gene="rpsS"
                     /locus_tag="PLB46_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRF76523.1"
                     /translation="MARSIKKGPYVDHNLAGKVEKLNESGKKTVVKTWSRRSTIFPDM
                     VGHTFAVHNGNKFIPVFVTENMVGHKLGEFSPTRNFKSHSGNR"
     gene            5481..5873
                     /gene="rplV"
                     /locus_tag="PLB46_08125"
     CDS             5481..5873
                     /gene="rplV"
                     /locus_tag="PLB46_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRF76524.1"
                     /translation="MEAVAKLINNPRTNNNAGSPRKTRYVVDLIRGLDIDKALNVLRF
                     TDKAAATPIEKLLRSAIKNWEQKFEMDAADSSLYVKECFVDGGKTMRRFQPAPHGRAY
                     RIRKRTNHITIKVASRTATNNANNETKE"
     gene            5888..>6372
                     /gene="rpsC"
                     /locus_tag="PLB46_08130"
     CDS             5888..>6372
                     /gene="rpsC"
                     /locus_tag="PLB46_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRF76525.1"
                     /translation="MGQKCNPIGNRLGIIRGWDSNWYGGKTMAEKLVEDERIRQYLSA
                     RISKGGISKIVIERTLNRITVTIHTSRPGIIIGKGGGEVDKVKEELKKITDKDVQINI
                     IEIRRPELDAAIVGETIARQLEARINFRRAIKMAIASTMRMGAEGIKIRISGRLNGAE
                     MA"
BASE COUNT         2159 a         1203 c         1402 g         1598 t
ORIGIN      
        1 gtagaaaaac aaaccggtga aaatggttac gaagtttgga aaaaagcatt gagcaacgta
       61 actcctggtg ttgaggtaaa aagccgccgt attggtggtg ctaacttcca gattcctaca
      121 gagattcgcc ctgaccgtaa gatttcatta tctatgaaat ggttaatcat gtttgctcgc
      181 aaacgcagcg gtaaatcaat ggcagaaaaa ttagccgctg aagttgttgc tgcagcaaaa
      241 ggtgaaggtg caacaattaa gaaaaaagaa gatgttcata aaatggctga agcaaacaaa
      301 gcttttgctc actttaaatc ataataatta gttaaagaca aatacaatgg caagagattt
      361 aaaatacact cgtaacatag ggatcgcggc gcatatcgat gccggtaaaa cgactaccac
      421 tgagcgtatt ttgttttata ctggtatcac gcacaaaatc ggtgaagtac acgatggtgc
      481 tgctactatg gactggatgg agcaagagaa agaacgtggt attaccatta cttctgctgc
      541 tacaactact cactggaaat atagaaacga agaatataaa gttaacatta tcgatactcc
      601 cggccacgtg gattttaccg ttgaggtaga acgttcactc cgcgtattag atggtgttgt
      661 tgcgttattc tgtgcagttg gcggtgttga accacaatct gaaacagttt ggcgtcaggc
      721 taaccgttat aaagttcctc gtttaggttt cgtaaacaaa atggaccgtt ctggtgccga
      781 ttttcttgaa gttgttaaac aagtaaaaga aattttaggt gctaatccgg ttccactggt
      841 attacctatc ggtgcggaag aaacatttaa aggtattgtt gatttaatca ccaacaaggc
      901 tatcatttgg gatgatgaaa ctcagggtat gacttacaaa gaagtgccta ttccggatga
      961 tatgaaagac ctcgttgttg aataccgtaa tcacctcgta gaagctgttg ctgaatacga
     1021 tgatgcatta ttggaaaaat tcttcgacaa tcctgattca atcacagaag aagagttgat
     1081 tacggctatc cgtaaagcaa ctatcgatat cgctatcatc ccaatgttat gcggttcatc
     1141 tttcaaaaac aaaggtgttc agtttatgtt ggatgcagtt gttcgttatt tgccttcacc
     1201 actcgatatc gatgcagtga aaggtactaa cccggacact gaagaagaat tgtctcgtca
     1261 gccggattct aaagaaccgt ttacggcttt agcattcaaa attatgactg acccattcgt
     1321 aggtcgtttg gcattcttcc gcgtatatgc cggtaaatta gatgctggtt catacgtatt
     1381 aaacgtaaga acaggtaaaa aagaacgtat ttctcgtatc cttcaaatgc atgccaataa
     1441 acaaaaccca ctcgatacta tcgaagcagg tgatattgga gcagcagttg gttttaaaga
     1501 tatcagaaca ggtgataccc tttgcgatga gaacaatccg atcgctttgg aaagtattaa
     1561 attccctgat ccggttatcg gtttggcaat tgagcctaaa actcaggccg acgtagataa
     1621 actcggtatg gctttagcga aattagcaga agaggatcct acattcaaag taaactttga
     1681 tgaagaaaca ggccaaactg tagttcgcgg tatgggtgag ttacaccttg aaattatcgt
     1741 tgaccgctta aaacgcgaat ttaaaattga agttaatcag ggtgctcctg aggttgctta
     1801 taaagaatca tttaccaaaa cggttaaaca ccgtgaggtt tacaaaaaac aaaccggtgg
     1861 tcgcggtaaa ttcgctgata tccagtttga aatcgggcct gctgatgagg gagttgttgg
     1921 tttacagttt gtaaacgaca tcttcggagg tgctatccct agggaatatg taccatcagt
     1981 tgaaaaagga tttaaagaag caatgtcaac aggcgtttta gccggctaca caatggacag
     2041 cttaaaagtt cgtttgttcg acggttcatt ccacgcagtt gactctgatg cattctcttt
     2101 tgagatggca gctaaaattg ctttccgtga tgcaggtaaa aatgcaggac ctcaattact
     2161 tgagcctatc atgaaattag aagttattac tcctgaagat catatgggtg atgtaattgg
     2221 tgacttaaac cgtcgtcgtg gtatgattga aggttttgat aacaaaaaca acgcaaccgt
     2281 tatcaaagca aaagttccac tgagtgaaat gtttggttat gtaacacaat taagaaccat
     2341 cacttcagga cgtgcaactt caattctcga gttctcacac tttgcaccgg cgccagcgtc
     2401 agtgtctgaa gaggtgatag ctaaatcaaa aggaaaagta acagttaatt gataaaagta
     2461 agaatacagt agtatgacct ataaaatcag aatgaaactg aaatcttttg accacacttt
     2521 ggttgacaaa acggctgaga aaatcgtaaa gaccgttcgc aacacaggtg cagtggttac
     2581 gggaccaatc ccgctgccta cgcagaaaaa gatttacaca gtattgcgtt ctcctcacgt
     2641 taacaagaaa gcacgtgagc agttccaaat gtgtacctat aaaaggttgc ttgacattta
     2701 cagtgcaacc aacaaaacag tagatgcatt gcagaaactg gagttaccaa gtggagtaga
     2761 agttgaaatt aaaatctaaa agattctcaa gaaaatgaaa ggaatcatag gcaaaaaact
     2821 cggcatgacg cacttttacg gtgaggaagg aaaattaatt tcctgcactg taatcgaagc
     2881 cggaccatgc gtggttgtgc aaaagaaaac cgacgatact gatggcnnnn nnnnnnatgg
     2941 ctataatgct gttcagttag gttttgagga attgaaggaa gtacgtacca acaaggctca
     3001 aaccggtcac ttcaaaaaat caggcgctaa accaatgcgc aagattaagg agttccgcaa
     3061 ctttgatctc gataaaaatg taggtgataa catcactgtt gacatttttg aagaaggtga
     3121 aaaagtgagt gtagtgagca cttcgaaagg taaaggtttc cagggtgttg taaaacgcca
     3181 caactttagc ggtgtaggta tgcgcacaca cggtcagcac gatcgtcatc gcgcacctgg
     3241 ttcaatcgga caatcatcat atcctgcgag agtattcaaa ggattgagaa tggccggtcg
     3301 tatgggtaac gaaaccgtta aacaacgcaa cctcaaagta gcaaaaatta tttctgacaa
     3361 gaacctgatc ttaattaacg gatcagttcc gggaagcaaa ggttcttacg taataatcga
     3421 gaaataaaat gaaagtagaa atcgtaaaaa tatcaggcga aaaaaccggt aaacaggttg
     3481 agctcaacga agaaatcttc ggtgttgaac caaacgacca cgctatttac ctttcagtaa
     3541 agcaatacct tgctaaccaa cgccagggaa cgcataaatc gaaagaaaaa ggtgaagctg
     3601 caggttcaac ccgcaaattg caccgtcaaa aaggaacagg tggatcacgt aaaggaagta
     3661 ttaaaaaccc tttattccac ggtggtggta ctatatttgg cccacgtcct cgcgattaca
     3721 gctttaagct taaccgcaaa gtgaaagaat tagcacgtcg ttctgcttta tcttataaag
     3781 caaaacaaaa caacattgtt gtagtggaag atttcacttt ggatacacca aaaactaaaa
     3841 cctttgccgg tattatgaaa aacattggtg ttagcgacaa aaagacatta tttgtattag
     3901 gtgataacca aaatatcaga ttgtcattgc gcaatttacc aaaaacaggt gcttcaaaag
     3961 caagtgacct taacacctac gacatcatga atgcacagtg tatcgtgtta agcgaaagca
     4021 cagtgaatct gatcaatcaa atggcagaaa acaactaata aaataagtaa aatggcaaag
     4081 agaatattaa taaaaccact cataaccgag aaaaccacca agctttctga agctaatggt
     4141 acctatggtt ttgtagttgg tatggatgcc aataaaatag agatcaaaaa tgccgtggaa
     4201 aaaaactacg gtgttacagt ggcggcggta aaaacgatta cagacaatgg taaattcggc
     4261 tggaaaagaa ctccgttcgg gatcagtcag ggcaccaaag gacgtatgaa aaaagcttac
     4321 gttactctca aaaaaggcga aacaattgat tttttcagca caatttaatc tgaaagatgg
     4381 ctttaaagaa atataatccg gttacaccag gtacacgatt cagaataggc aatgcttatg
     4441 ctgaggttac tacggataca ccagaaaaga gtttgacagc cccgtttaag aaaacgggtg
     4501 gacgcaatag ttcgggagca ttgaccatgc gctatattgg cggtggccat aaaaagaaat
     4561 accgtatcat tgactttaag cgcgacaagc ataatgttcc cgctacagtt aaaacggtag
     4621 aatacgatcc gaaccgtact gcatttatcg cattggttaa ctatgccgat ggtgaaaaac
     4681 ggtatatcct ggcaccaaat ggtattcagg taggacaaac cattgaggca ggcgacaatg
     4741 ttgctcctga attaggtaac gctttgatgc tccgtaatat cccgctcgga actatcatcc
     4801 acaatatcga gatgcaaccc ggtaaaggtg gtatgattgc ccgcagcgca ggaagctggg
     4861 ctcagttatc agctaaggaa ggtaaatatg taattgttcg tatgccttca ggtgaaacac
     4921 gtatgatatt ggacacttgc tatgcaactg ttggtacggt ttctaaccct gaccacaact
     4981 tacagcaact tggtaaagca ggtcgcaatc gttggttagg cgttcgtcct cgtaaccgtg
     5041 gtgttgccat gaaccctgtt gatcacccaa tgggtggtgg tgaaggacgt tcatcaggag
     5101 gacatccaag gtcacgtaac ggtaaatact caaaaggtat gaaaacccgt caccctaaga
     5161 aaacaacatc taagtttatt attagcagac gcaaaaaata attaatagtt aatggcacgt
     5221 tcgataaaaa aaggacctta tgtggatcat aatctcgccg gcaaggtaga gaagttgaat
     5281 gaaagcggta aaaaaactgt ggttaaaaca tggtcacgcc gttctaccat tttccctgat
     5341 atggtaggtc atacattcgc agttcacaat ggcaacaagt tcatcccggt atttgttacc
     5401 gagaacatgg taggtcataa attaggtgaa ttttcaccaa caagaaactt taaatcacac
     5461 tctggtaaca gataagaatt atggaagcag tagcaaaact tataaacaac ccaagaacca
     5521 acaacaacgc aggttcgccg cgtaaaacaa gatatgttgt tgaccttatc agagggttgg
     5581 atattgataa agcactcaat gtgttaaggt ttactgataa agcagccgca acaccaattg
     5641 aaaaattgtt gagaagtgca attaagaact gggagcaaaa attcgaaatg gatgctgccg
     5701 acagtagttt atatgtgaaa gaatgttttg tagacggagg taaaaccatg cgtcgttttc
     5761 aaccggcacc acacggaaga gcttaccgta tccgcaaacg caccaatcac attactatta
     5821 aagttgcatc aagaactgca accaataacg caaataacga aaccaaagaa taaaataatt
     5881 aattcgaatg ggacaaaaat gtaacccgat aggcaacaga ttaggtatca tccgcggatg
     5941 ggacagcaac tggtatggtg gaaaaaccat ggcagagaaa ctggtagaag atgaaagaat
     6001 ccgccagtac ctgagcgcca ggatatcaaa aggtggtatt tcaaaaatcg tgattgaaag
     6061 aaccctcaac agaatcactg taacaatcca tacttcacgt ccgggaatta tcatcggaaa
     6121 aggtggtgga gaagttgata aagtgaaaga ggaattaaag aaaatcactg acaaagatgt
     6181 acaaatcaac atcatcgaaa tccgtcgtcc ggaattagat gctgcaattg taggcgaaac
     6241 aatcgctcgt cagttagaag cacgtatcaa tttccgccgt gccattaaaa tggctatcgc
     6301 atcaaccatg cgtatgggag ctgaagggat caaaatccgt atcagcggtc gtttaaacgg
     6361 agctgagatg gc
//
LOCUS       DAORKW010000171         6357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_435_length_6357_cov_4.413202, whole genome shotgun sequence.
ACCESSION   DAORKW010000171 DAORKW010000000
VERSION     DAORKW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_435_length_6357_cov_4.413202"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1413)
                     /locus_tag="PLB46_08135"
     CDS             complement(31..1413)
                     /locus_tag="PLB46_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76526.1"
                     /translation="MKNIVILFCCAMLTIPAAAQTTFTDELDKLIAEGMLSTAEGKVD
                     SALMINPGNVDALYYKGNIQYYKYGSYADLMKTGGTPDESVYTSDYIYIGQYKEILPK
                     PIADTCAFFFSNALKVDPGRDDIRLALGYIYAISLQDNKLIELLPSMTKVPAINDNSI
                     RDYAYNFLDRGAYQQGMNVYEKISSLYPDNGNLLCDMAVEYYIIGDIQKAIANAKKCY
                     TMPNLDTITYSNAFFLFSVAEQHEEAIACIQIWSNMVNNKTALLYKGLYELLSNQSTA
                     KKTLNEYVQTELLASAEKDFALFLLNNDLQINADKLDTIYYFGLTDAFSMLLYHHFYN
                     KYPDAFLPGYNYAESLTYNKRYTEAAQVFSKINTAATSGHDIDEYHFYYAWALHQSGD
                     DETANLHWLEAAKSNIPYLQGGANYFLGKYYYNKNDFVNARKYFLSGANASSESKFSA
                     YCSDFLSILK"
     gene            complement(1440..1868)
                     /locus_tag="PLB46_08140"
     CDS             complement(1440..1868)
                     /locus_tag="PLB46_08140"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF021205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HRF76527.1"
                     /translation="MAYTIPLIIKTIPPKGYHIFVDGKIGRHKMRFLIDTGASKSVIN
                     QDYANNQFTGSLITETDHLTTGLGANIPNSSFIRLHRVKLDKFSIKTVQFAILDLSMV
                     NQAYTSAGLNPVEAIIGGDILKKYGAIIDYSNRQLIMNQP"
     gene            complement(1942..2886)
                     /gene="mdh"
                     /locus_tag="PLB46_08145"
     CDS             complement(1942..2886)
                     /gene="mdh"
                     /locus_tag="PLB46_08145"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRF76528.1"
                     /translation="MSKVTVIGAGNVGSTVANVLAHRDFLNEIVVLDIKEGTAEGKSL
                     DTWQQAPIDNYSTRVVGCTNDYAKTADSDVVVITSGLPRKPGMSRDDLISVNAGIVKT
                     VTEQVIKYSPNAIIIVVSNPLDVMTYCAYLTAKVDSHRVFGMAGILDTARYRAFLATE
                     LNCSPKDIQAILMGGHGDTMVPLPRYTTVSGIPVTELISEEKLNAIVQRTKVGGGEIV
                     NLLGTSAWYAPGAAAAQMVEAIIKNENRIFPVCALVNGEYGLHNIYLGVPVKLGKNGI
                     EQIIELQLNNDEKALLNASATAVKEVMEVLDKMNASAS"
     gene            3026..4456
                     /locus_tag="PLB46_08150"
     CDS             3026..4456
                     /locus_tag="PLB46_08150"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76529.1"
                     /translation="MRKLLLFIPLISGLLSLQAQSQYCGTLQTEEDLQWLRHHQQNYV
                     PNEDNRGGTTYYIPVKVHIVGSDEGTGYYNLSYMYTQFCELNEHYAATGFQFYIYGDL
                     DYIDNTDYYIHDWFDGEDMMDNNNINDVVNIYFVSDPAGNCGYFSPSDDGVAVAKSCA
                     MPGGTTIAHEFGHFFSLPHTFYGWEWGTPDDNDQEWVNGDNCNFTADGFCDTPPDYLP
                     YRWNCPGPMQTDPHGETFYSDGTFYMSYSNDECTDKFSEEQQDAMRANLLGPRNDMLD
                     FPAPTYVDITETPVLLAPEDMATNTYMNYTYIGWTAVEGATQYHLQLAYNSTFSAVAF
                     DIVTTNNYYEARDLLGDKKYYWKVRPLAPLNTCEPTSESRSFTTGNSFSAIAETAIFD
                     HLTVFPNPAKAGGSVTLSYRTSVGFDGAAAILDITGKQISQQNIQVIGGAGSINLTLP
                     EIAEGIYFIKLSDDKQSELIEFIVTQ"
     gene            complement(4487..5842)
                     /gene="tilS"
                     /locus_tag="PLB46_08155"
     CDS             complement(4487..5842)
                     /gene="tilS"
                     /locus_tag="PLB46_08155"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRF76530.1"
                     /translation="MINTSTFMQFVTANNLCSKNDHILLAVSGGADSMVMCDLFLQSG
                     FTFGIAHCNFQLRKEESDADEQLVFNYAQKNNIPFYAQRFDTVAYSKLNKISIEEAAR
                     DLRYAYFAALCDEHGFHLVATAHHANDNAETILMRLAKGTGIKGLQGIPVKNNQIIRP
                     LLFATKNDIAEYCITHQIAYRDDASNFSDHILRNYFRLNIIPQLEKINPSFVPTMREN
                     VLHIEGAFHFYEQAVTQRLHKIVSHKGNDKYIAIAALGDTLSAGVLLHELLAPIGFNA
                     SQIKQIIAAFGQTGKQFFSEKYRVIIDRKHIIITERSNIQNSLQFIEAGLKKIAVNNF
                     YLQFEITVFNKSMNFNSDHHIAYFDADKITFPLTIRRWKAGDYLYPLGLTKRKSDKPG
                     KKKVSDILTNAKADTLAKENTFVLLSGEKIIWLIGYRQDERFKITDSTRNLLKIKMLP
                     K"
     gene            complement(5835..6164)
                     /locus_tag="PLB46_08160"
     CDS             complement(5835..6164)
                     /locus_tag="PLB46_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76531.1"
                     /translation="MSRINQLLQFLQDNPKDSFIQYALALEYIKNGDLVTGRQYFEQL
                     ISNDPEYVGTYYHLAKLYVQLNEKSLAENCYVNGIAIAKKLNDTHSLAELQNAYTNLK
                     MGISDDD"
BASE COUNT         1959 a         1151 c         1098 g         2149 t
ORIGIN      
        1 agttactaat aaataaacag cccctttttt ttatttaagg atggacaaaa aatcgctgca
       61 ataagcagaa aatttcgatt cactacttgc attagcgcca cttaaaaagt atttacgtgc
      121 attgacaaaa tcatttttgt tgtaataata tttacctaaa aaataattag ctcctccctg
      181 aaggtagggg atattagatt tagcagcttc taaccaatgt aaatttgctg tttcatcatc
      241 accggattgg tgtaaagccc atgcataata aaaatgatat tcgtctatat cgtgaccact
      301 tgttgcagct gtgtttattt ttgaaaacac ttgagcagct tctgtatatc gtttgttata
      361 ggttaaactt tcggcataat tatatccggg taaaaaggca tcagggtatt tgttataaaa
      421 atgatggtag agtaacatac tgaatgcatc tgttaaccca aaataatata tagtatctaa
      481 tttgtcagca ttaatttgca aatcattatt tagtaaaaat agggcgaaat ctttttctgc
      541 tgaagctagg agttctgttt gcacatattc atttaatgtt tttttagctg tgctttgatt
      601 gctcaataat tcatataatc ctttgtataa caacgctgtt ttattgttaa ccatattgga
      661 ccaaatttga atacatgcaa ttgcttcttc gtgttgttct gcaacactaa acagaaaaaa
      721 agcattacta taagtaattg tatccagatt aggcatggta taacattttt tagcattggc
      781 gatagccttt tgtatgtctc caatgatata atattcaacg gccatatcgc ataacaaatt
      841 accattatca ggatataagg agcttatttt ttcatatacg ttcatgcctt gctggtaagc
      901 acctctgtct agaaaattat aagcatagtc tctgatggaa ttatcgttaa ttgccggtac
      961 ttttgtcatg gagggtaata attcaatgag tttattatcc tgtaaactaa tagcataaat
     1021 gtatcctagt gccagtctga tatcatctct gcccggatca actttaagcg cattactaaa
     1081 aaagaatgca caggtatccg caattggttt aggtaaaatt tctttatact ggccaatata
     1141 aatataatca gaagtataaa cagattcgtc aggagtgcca cccgttttca ttaagtcggc
     1201 ataacttcca tacttataat attgaatatt gcctttgtaa taaagtgcat caacatttcc
     1261 gggatttatc attaaggcag aatcaacctt accttcggct gtacttagca taccttcagc
     1321 aatcagttta tcaagttcgt cggtaaatgt agtttgtgca gctgcaggaa ttgtaagcat
     1381 agcacaacaa aatagtatta caatgttttt catctggtat attaagtaat aaatctaatt
     1441 tatggttggt tcataattaa ttgtctattg ctataatcaa ttatcgctcc atattttttt
     1501 aaaatatcgc caccaataat tgcttctacc ggatttaatc ctgccgatgt ataagcctga
     1561 ttaaccatag ataaatctaa aatagcaaat tgcacagttt ttatggaaaa cttatccagt
     1621 tttacacgat gcaatcggat aaagctgctg tttggaatat ttgcgcctaa ccctgttgtc
     1681 aggtgatctg tctcagtgat taaactaccg gtaaattggt tgttggcata atcctgatta
     1741 attacacttt tactggcacc ggtatcaatt aaaaaacgca ttttatggcg tccaattttg
     1801 ccatcaacaa aaatatgata cccttttggt gggatggttt tgatgattaa tgggattgta
     1861 tatgccatat gattacaata aaaaaccgca aaaccttttt tatgatttgg gttttgcggt
     1921 ttgtgaaata gttaaaaaca attatgatgc tgaagcgttc attttatcca atacttccat
     1981 aacttctttt acagcagttg cagatgcatt taacaatgct ttttcatcat tatttaattg
     2041 taattctata atttgttcaa taccgttttt tcctaattta accggaacac caaggtagat
     2101 attatgtaaa ccatattctc cgtttactaa agcacaaaca gggaatattc tgttttcatt
     2161 ttttataatt gcttctacca tttgcgcagc tgcagctcct ggtgcatacc atgcagaagt
     2221 gcctaataag tttacaattt caccaccacc aactttagtg cgctgaacta tcgcgtttaa
     2281 tttttcttcg ctaattaact cagtcaccgg aataccacta actgttgtat aacgtggaag
     2341 cggcaccata gtatcgccat ggccacccat taaaattgcc tgaatatctt ttggtgaaca
     2401 attcaattct gttgctaaaa acgcgcggta acgagctgta tctaaaatac ctgccattcc
     2461 aaatactcta tgactgtcaa ccttagcggt taaataagca caataggtca ttacatccaa
     2521 aggattagaa accacaataa tgatggcatt tggcgaatac ttgataacct gttcagtaac
     2581 agtttttaca ataccggcat tcacactaat taaatcgtcg cggctcattc ccggtttacg
     2641 cggtaagcct gatgtaatta ctaccacatc tgaatcggcg gttttagcat aatcattcgt
     2701 acagcctaca acacgagtgc tatagttgtc gataggggct tgttgccagg tatctaaact
     2761 tttgccttct gctgttccct ctttaatatc taataccaca atctcattca aaaagtcgcg
     2821 gtgagcgagc acattggcaa cagttgaacc cacattgccg gctccgataa cggttacttt
     2881 actcatagtt ttgtcatttt acctgcctaa ttacgcttag gcgctgcaaa gttatgcctt
     2941 ttaggctgaa agatggaatt gtgcataaac tttgctggta attttacgtt tgacttttta
     3001 actttacaca ataatttaac atttcatgag gaaactgcta ttatttatcc ctctaatttc
     3061 gggtttatta agtcttcagg cgcaatcaca gtattgtggg acccttcaaa ctgaagaaga
     3121 cttgcaatgg ttgcgtcatc atcagcaaaa ttatgttcct aacgaggaca accgaggagg
     3181 taccacctat tacattcccg ttaaagtgca cattgttgga agcgatgaag gaacaggata
     3241 ttataactta agttatatgt atactcagtt ttgcgaactt aacgaacact acgctgctac
     3301 tgggtttcaa ttttatattt atggagattt agattatata gataataccg attattatat
     3361 ccacgattgg ttcgatggtg aagatatgat ggataataac aacattaatg atgttgtcaa
     3421 tatctacttt gtatctgacc ctgccggcaa ttgtggttac ttcagcccat ccgatgatgg
     3481 cgttgctgta gcaaaatcat gtgctatgcc tggtggaaca actattgcac acgaatttgg
     3541 gcacttcttt tcattgccac atacatttta tggctgggaa tggggaacac cggacgataa
     3601 tgaccaggaa tgggtaaatg gcgataattg caattttaca gctgatggct tttgtgatac
     3661 accaccggat tatttgcctt atcgttggaa ttgccctgga ccaatgcaaa ctgacccaca
     3721 tggggaaaca ttttacagtg atgggacatt ctatatgtct tattcaaatg atgaatgcac
     3781 cgacaaattt tccgaagagc agcaggatgc catgcgcgca aatttattag gaccaagaaa
     3841 cgatatgctc gattttccgg ctccaactta cgttgatata actgaaactc cggtattact
     3901 tgcacctgaa gatatggcaa ccaatactta tatgaattat acttatatag gttggactgc
     3961 tgttgaaggt gcaacacaat atcacttaca attggcttat aattctacat tcagtgcagt
     4021 tgcttttgat attgttacca ctaataatta ctatgaagct agagatttgt taggcgataa
     4081 aaaatattat tggaaagtgc gtcctttagc tccactcaat acctgtgaac caacttctga
     4141 aagtcgttcg tttacaacag gtaattcatt tagcgctatt gcagaaacag ctatttttga
     4201 ccacttaacc gtatttccga acccagcaaa agctggtgga agtgttacac tttcttatcg
     4261 tacttcagtt ggttttgatg gagctgctgc gattttggat attaccggca aacaaatcag
     4321 tcaacaaaat attcaggtta ttggtggtgc aggttcaatt aacttgactt taccggaaat
     4381 tgctgaaggg atatacttca ttaaattatc tgatgacaaa cagtctgaat taattgaatt
     4441 tatagtaacg caataacgat attaatttct aaaaataaaa tgctccttat tttgggagca
     4501 tttttatttt aagcaagtta cgtgtagaat cagttatttt aaaacgctca tcctggcgat
     4561 aacctataag ccagataatt ttttcacctg ataataatac aaaagtattt tctttagcta
     4621 gcgtatctgc tttagcattg gtaagaatat cgcttacttt tttctttccc ggcttatcag
     4681 atttacgttt tgttaatccg agcggatata aataatcacc tgctttccat cttctgattg
     4741 ttaatggaaa agttatttta tcggcatcaa aataagcaat atgatgatct gaattaaaat
     4801 tcatggattt attaaacact gtaatctcaa attgcaggta aaaattattt actgctatct
     4861 ttttaagacc ggcttcaata aattgtaaag aattttggat attacttcgt tctgtaatga
     4921 tgatatgttt gcgatctata atcaccctgt atttttcact gaaaaattgt ttgcctgttt
     4981 gtccgaatgc tgctattatt tgttttattt gacttgcatt gaagccaata ggagcaagaa
     5041 gttcatgtaa taacacaccg gcgcttaaag tatcgccaag cgcggcaatt gcaatatact
     5101 tatcattgcc tttgtggcta acaattttgt gtaatctttg agttacagct tgctcataaa
     5161 aatgaaaagc accttctatg tgtaacacat tttcgcgcat agttggaaca aaagaagggt
     5221 ttattttttc caattgaggg atgatattta aacggaaata attgcgtaaa atatggtctg
     5281 aaaaattact tgcatcatct ctgtatgcaa tttgatgtgt aatacaatat tcagcaatat
     5341 cattttttgt tgcaaatagc aacggtcgaa taatttgatt attttttaca ggaattcctt
     5401 gcaaaccttt aatacctgtt cctttcgcca accgcattaa aatggtttca gcgttatcgt
     5461 ttgcatggtg agctgttgca actaaatgaa aaccatgttc atcacataaa gccgcaaaat
     5521 aggcatatcg caaatcgcgc gcagcttctt caatagatat tttatttaat ttactgtaag
     5581 caacagtatc aaacctttgt gcgtaaaagg gaatattgtt tttttgcgca tagttaaata
     5641 ccaattgttc atctgcatca ctttcttcct ttcttaattg aaaattacaa tgtgcaatac
     5701 caaaggtaaa accagattgt agaaacaagt cacacattac catgctgtct gcaccgccac
     5761 tcaccgccaa caaaatatgg tcgtttttac tacacagatt atttgcagta acaaattgca
     5821 taaatgtgga agtattaatc atcgtcgcta atacccattt ttaaattggt gtatgcattt
     5881 tgcaattcgg ccaggctatg tgtatcgttt aattttttgg caatggcaat tccgtttacg
     5941 taacaatttt ctgctaatga tttttcgttg agttgcacat ataattttgc caaatggtaa
     6001 tatgtgccta catattcagg atcgttagat ataagttgtt caaagtattg acgaccggta
     6061 acaaggtcac catttttgat atactctaat gcaagcgcat attgaataaa tgaatcttta
     6121 gggttatcct gtaaaaactg caacaattga ttaattcggc tcatatttca acggtaatgt
     6181 gtgcaaatat aactatagca tgctgtttcc tatttagtgc cggattgaat gtgaagaaat
     6241 tgtggagtga aagtccatcc aatttactga aaatctcgcc ctaaaacccc tatctttgca
     6301 gtccgaaaaa tgtaaaaata ccatatgaaa ttactggttt gtataagtaa agcccct
//
LOCUS       DAORKW010000172         6309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_439_length_6309_cov_4.927406, whole genome shotgun sequence.
ACCESSION   DAORKW010000172 DAORKW010000000
VERSION     DAORKW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_439_length_6309_cov_4.927406"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3042)
                     /locus_tag="PLB46_08165"
     CDS             complement(<1..3042)
                     /locus_tag="PLB46_08165"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRF76532.1"
                     /translation="MQNKKVLLLIFYLLSIEAYSQEFEWAKQIGGLNAQVTEAIITDS
                     TGNVYTAGWFRGTVDFDPGPGIFNLSCSPELGTDILENDIFISKLDAEGNFVWAKKIS
                     GDGYQGAHALALDAEQNIYVTGRFSHTVDFDPGPATFNITSSYNDLPDAYIANMFLLK
                     LDNAGNFVWAFGVSDIALGNLYGQHIVLDEEANIYVAGYFSETSDFDPSPDIALLEKG
                     GIWDVFVAKYNTDGNYLWAKKLGGTGGEYSFSLTRDLEGNIITAGYFFGSGDYDPGPG
                     EYILTSDGITDFETFVSKLDSDGNFIWVNHLVGDNDVMAKSVVTDGNDIIVFADFKDV
                     FDADANAGVYELTSVGASDIMVAKYDADGLLLWAKSIGGLADDIPYEGVVDVMGNIYF
                     TGYFNGTIDVDPSPAIFNLTADLTDGFICKWDADGNFIWATKIGGVGSDYGKGIFVDN
                     SYNIYLTGYFDNTVDFDPGPANFPLTCEADDVFVLKLTQDYCSTLTLITDTVGDVLCG
                     SSALLAGHADGGHAPYTYVWNTIPITYDSVATTPVGGLIQFKVTDAIGCVHTSKYFVN
                     GPTVIDGFDLKTNLIFGDIRDDFESNIWLDVFNNGCVATTGYVDVVLPIELNYVDATP
                     APDIIIGDTLRWLFTDLKFGDPHVTAHITVITSIFGQLDDLISINSAVRPAMDDVFPG
                     NNFKHYVIPIIGSYDPNDKAIYPQGICDDGYILNTDTLTYTIRFQNTGTAEAINIFIL
                     DTLSTLLDINTFKIVASSHAMVTELLPDNVLKFKFDNIYLPDSGANEIESHGYVIFEI
                     LPLPGLANFSVIDNNAAIYFDFNEPIITNSTHNTLLDELPILAVTQEYSICEGDFVTV
                     NGNNYNAVGIYIDTLISSFGCDSIVTTNIMNVNPAQYITNMVNICEGEVVTVGDNTYT
                     TTGLYIDNFANIFGCDSVILTDLTVHPTFTSTIEAEFCEGETYVFNGSTLSNPGLYYA
                     NLLSQDNCDSIVELTLTNHIVDAGVTATEFTLTANHADASYQ"
     gene            complement(3593..3844)
                     /locus_tag="PLB46_08170"
     CDS             complement(3593..3844)
                     /locus_tag="PLB46_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRF76533.1"
                     /translation="MYYTYVLRSMKNGVLYKGQTQDLVSRLEVHNAGMVRYTKKYMPW
                     ELVYFETFNTRAESMAREKYFKTGTGREWLKKHLESIGK"
     gene            complement(3957..4208)
                     /locus_tag="PLB46_08175"
     CDS             complement(3957..4208)
                     /locus_tag="PLB46_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRF76534.1"
                     /translation="MYYTYVLRSMKNGVLYKGQTQDLVSRLEVHNAGMVRYTKKYMPW
                     ELVYFETFNTRAESMAREKYFKTGTGREWLKKHLESIGK"
     gene            complement(4264..4337)
                     /locus_tag="PLB46_08180"
     tRNA            complement(4264..4337)
                     /locus_tag="PLB46_08180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4301..4303),aa:Arg,seq:acg)
     gene            complement(4596..5585)
                     /gene="floA"
                     /locus_tag="PLB46_08185"
     CDS             complement(4596..5585)
                     /gene="floA"
                     /locus_tag="PLB46_08185"
                     /inference="COORDINATES: protein motif:HMM:NF010186.0"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HRF76535.1"
                     /translation="MDSNQIVLILVIVVGFAILIFFLIFMRLFGLWLSAQLAGVKISL
                     LQMILMRWRRVDPRAIVDNMVTAHKAGIPLTRDQLEAHFLAGGNIKRVVNALVSADKA
                     NIPLDFKAATAIDLAGRDVFEAVQLSVNPKVIESPPVTAVAKNGIQLIAKVRVTVRAN
                     IQKLVGGAGEETILARVGEGIVTTIGSSENHRAVLENPDTISKTVLAKGLDSGTAFEI
                     LSIDIADIDIGKNIGAELQMDQANADKNIAQAKAEERRAMAVASEQEMKAKAQEARAK
                     VIEAEALIPMAIAEAFRNGNLGVMDYYKLRNLQADTEMRDALGKGQGKKDDSK"
     gene            complement(5592..6062)
                     /locus_tag="PLB46_08190"
     CDS             complement(5592..6062)
                     /locus_tag="PLB46_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRF76536.1"
                     /translation="MSLALIITIILVGVIFIFVEFFFVPGFSVFSILGAIVAGIGVYM
                     GYNQYGQTAGNWLLLSALLAAGGIFYWGYKRMQSKKWALKTEIDGKVNVEDFANFKLG
                     DKGVTLTNLRPEGKAIFGDDERITVYSIGEFIDKDAQVTIVKIEHNKIFVTTVN"
BASE COUNT         2014 a         1325 c         1048 g         1922 t
ORIGIN      
        1 ctgataactt gcatcagcat gattggcggt gagggtaaat tctgttgccg ttacacctgc
       61 atctacaata tgattagtaa gcgtaagttc tactatcgaa tcacagttgt cctgtgaaag
      121 taaattggca taatataatc cgggattaga taatgtacta ccattaaaaa cataggtttc
      181 cccttcacaa aattcagctt caattgtaga tgtgaatgtt ggatgcaccg ttaaatcggt
      241 gagtatcaca ctatcacaac caaatatatt cgcaaaatta tcgatatata atcctgtggt
      301 ggtatacgtg ttatcaccaa cggtaacaac ttcaccttcg caaatattta ccatattagt
      361 tatatattgt gcagggttaa cattcataat atttgtggtg actatactgt cgcaaccaaa
      421 tgatgaaatg agtgtatcta tataaatacc aactgcattg tagttatttc cgtttaccgt
      481 tacaaaatca ccttcgcaga ttgaatattc ctgtgtgaca gctagaatag gcaattcatc
      541 taataaggta ttatgcgtgg aattggtaat tatcggttca ttaaaatcaa aataaatagc
      601 cgcattatta tcaattactg aaaaattagc gagaccagga agtggtaaaa tttcaaaaat
      661 cacataacca tgactttcaa tttcgttggc tccactatcg ggtaaataaa tattatcgaa
      721 tttaaatttt aatacattat cgggaagtaa ttcagtaacc atagcatgac ttgaagctac
      781 aattttaaat gtattgatat cgagtaatgt gctcagtgta tcaagaataa aaatatttat
      841 ggcttcagca gttccggtat tttggaatcg gatggtatag gtaagtgtat cggtgttaag
      901 tatataacca tcatcacaaa ttccctgagg ataaatggct ttatcgttcg ggtcatacga
      961 accgattatt ggtattacat aatgtttgaa gttattgccg ggaaatacat catccatggc
     1021 cggtcgcaca gctgaattga tggaaattaa atcatccaat tgtccaaaaa tactagtaat
     1081 gactgttata tgtgcagtga catgtgggtc accaaatttc aaatcggtaa ataaccatcg
     1141 caatgtatca ccaataataa tatccggtgc gggagttgca tcaacataat tcaattctat
     1201 aggcaacaca acatctacat aacctgtagt tgccacacaa ccattattaa aaacatcaag
     1261 ccaaatatta gattcgaaat catctcttat atctccaaaa attaaatttg tttttaaatc
     1321 aaatccgtca attacagttg gtccgtttac aaaatatttc gaagtatgca cacaacctat
     1381 tgcatctgta actttaaatt gtatcaatcc gcccacaggt gttgtggcaa ctgagtcgta
     1441 ggttatcggg atggtgttcc atacataagt ataaggcgca tgtccaccgt cagcatgtcc
     1501 tgcgagtagt gcggaacttc cacataaaac atctccaact gtgtcagtaa ttaaggtgag
     1561 ggttgagcaa taatcttgtg ttaatttcaa aacaaataca tcatcagctt cacaagttaa
     1621 tggaaaatta gccggtcccg ggtcaaaatc aaccgtatta tcgaaatagc cggttaaata
     1681 aatgttatac gaattatcta caaaaattcc ttttccataa tcgcttccaa caccaccaat
     1741 tttagttgcc caaataaaat tgccatcagc atcccattta caaataaatc catcagttaa
     1801 atctgcagtc agattaaata ttgcgggact cggatcaaca tctattgtgc cattaaaata
     1861 tcctgtaaaa taaatattac ccataacatc aacaacacct tcatacggaa tatcgtcggc
     1921 caatcctcca atagattttg cccataacaa taaaccatca gcatcgtatt tggccaccat
     1981 gatatccgat gcgcccaccg aagtgagttc gtaaacaccg gcattggcat cagcatcgaa
     2041 aacatcttta aagtcggcaa atacaataat atcattgcca tcggtaacca ccgatttagc
     2101 cataacatca ttatcaccaa caagatgatt tacccaaata aaatttccat cgctgtcaag
     2161 ttttgacaca aatgtctcaa aatctgttat accatccgaa gttaaaatat attctccggg
     2221 acccgggtca taatcaccgg acccaaagaa ataaccggcg gtaataatat taccttccaa
     2281 atctcttgta agtgaaaaac tgtattcgcc acctgtgcct cccaattttt ttgcccacaa
     2341 ataatttcca tccgtattgt attttgctac aaatacatcc cagattccac ctttttccaa
     2401 aagggcgata tcgggactcg ggtcaaaatc agaggtttca ctaaaataac ctgcaacata
     2461 aatattggct tcttcatcga gcactatatg ttgtccataa agattaccta atgcaatatc
     2521 gcttacacca aaagcccaca caaaatttcc ggcgttatcc aatttcaata aaaacatatt
     2581 agcgatatag gcatccggta agtcattata cgaggaggtg atattaaacg tagccggtcc
     2641 cgggtcaaaa tcgacagtat gggaaaatcg accagttaca tatatatttt gttcagcatc
     2701 taatgccagc gcatgagcac cctgataacc atcgccgcta attttttttg cccatacgaa
     2761 atttccctct gcatccagtt tagaaataaa tatatcattt tctaatatat cagtccccaa
     2821 ttcaggactg cacgacaaat tgaaaattcc gggacccggg tcaaaatcta ccgttccacg
     2881 aaaccaacct gcagtataaa catttccggt ggagtctgta ataattgctt ccgttacctg
     2941 cgcatttaaa ccaccaattt gtttagccca ttcaaattcc tggctgtatg cttcaatgga
     3001 tagtaaataa aaaatgagga ggagtacttt tttattttgc atggtgttta atgggtttca
     3061 agtttaaaaa tagcgatgaa ttaaatctag atgttgtatt caattgtcag ccttttgcaa
     3121 tagttttcgg ggcatatttt tgtgttggtg ttaaattgaa aatgtgttta tttcaaattt
     3181 cttaaatagg tgttttttat caaaatttag ttgtcgggat aaataatgaa ccgaaacaaa
     3241 aacgattaac tgtattctta aaatggcttt tagatgtatt tgactataaa attagcaatt
     3301 tgtaatgttt tcgccaattg aggtgctaaa tgatatgctg catttttatt acacacaaca
     3361 acatggaagg caggaaaaac ccatagtgga gtaagcagtt tcgttttatg tagaattggt
     3421 attaatgcat tcaaattaac atctagcgaa acccctaacc ttcccgctta ggcgggatgc
     3481 tctaatcagg tgaacatact tcaaaaaaaa atcctgctat aagcaggatt agtgtgacct
     3541 cgaatccgcc ccggcggaaa acccctaacc ttctgtctta tcgcaagcat tatcatttac
     3601 cgattgattc caaatgtttt ttcagccatt ctcttccggt gcctgtttta aaatattttt
     3661 ctcgcgccat tgactccgcg cgtgtgttaa atgtttcaaa atacactaac tcccaaggca
     3721 tatacttttt agtataccga accattcctg cattgtgcac ttctaagcgc gaaacaaggt
     3781 cttgagtttg acctttgtac aatacgccgt tcttcatcga acgtaataca taagtgtagt
     3841 acattgattg ttatttaagg gttaaacaaa aaaaatcctg catttagcag gattatagtg
     3901 acctcgaatc cgccccggcg gaaaacccct aaccttctgc cttatcgcaa gcattatcat
     3961 ttgccgattg attccaaatg ttttttaagc cattctcttc cggtgcctgt tttaaaatat
     4021 ttttctcgcg ccattgactc cgcgcgtgtg ttaaatgttt caaaatacac taactcccaa
     4081 ggcatatact ttttagtata ccgaaccatt cctgcattgt gcacttctaa gcgcgaaaca
     4141 aggtcttgag tttgaccttt gtacaatacg ccgttcttca tcgaacgtaa tacataagtg
     4201 tagtacattg attgtttttt aaaggttaaa caaaaaaaaa tcctgcattt agcaggatta
     4261 tagtgacctc gaagggattc aaacccctaa ccttctgatc cgtagtcaga tgctctattc
     4321 agttgagcta cgaggccata ttatgcttcc gccaaggcgg attgagctac gaggccataa
     4381 ttattacaaa acggagtgca aaggtacaat ttatctcaat aaattcaaaa taattatcca
     4441 attcactcac tatcaaacca ttataaataa ttcaaaacct atcaatcaaa tctccacatt
     4501 tcattctaac tcagacccgg ctgttaattg gtacattggt acatctccac atttgcacat
     4561 taatcagcac atcatcacat taccacatta gcacattact tcgagtcatc cttcttccct
     4621 tgccctttgc ctaatgcatc acgcatttcg gtatcagctt gaaggttgcg taatttatag
     4681 taatccatta cacctaagtt gccattgcgg aatgcttctg ctatagccat aggaattaat
     4741 gcctctgctt caattacttt agcgcgtgct tcctgtgctt tagccttcat ttcctgctca
     4801 ctggcaacag ccatggcacg acgttcttcg gctttagcct gagcgatatt tttatcggca
     4861 ttggcctggt ccatttggag ttcggcacca atgttttttc caatatcgat atcggcaata
     4921 tcaatggata aaatttcaaa tgcagttcca ctatctaaac cttttgccaa tactgttttt
     4981 gaaatagtat caggattttc gagcactgct ctgtggtttt cgctggaacc aatggtggta
     5041 acaatacctt caccaacacg agccaaaatc gtttcttcac cggctccccc tactaatttt
     5101 tggatgttag cacgaactgt aacacgaact ttagctatta actggattcc gtttttagct
     5161 acagccgtca caggaggtga ctcaataact tttgggttta cacttaattg cacagcttca
     5221 aaaacatcac gtcccgcaag gtcgattgca gttgctgctt taaaatcgag cggaatatta
     5281 gctttatcgg cagaaaccaa tgcatttacc acacgtttaa tattaccacc ggctaaaaag
     5341 tgagcctcca gctgatcgcg tgttaaagga ataccggctt tatgcgcagt aaccatatta
     5401 tccacgatag cacgtgggtc tacacggcgc cagcgcatca atatcatttg caagagactt
     5461 attttaaccc ctgctaattg agcagaaagc cataatccga acaatcgcat gaatatgaga
     5521 aagaaaatca ggatcgcaaa cccgactaca atgactaaaa tgagcactat ctgattagaa
     5581 tccatatata tttagtttac agttgtgaca aatattttat tatgttcaat ttttacaata
     5641 gttacctgag catctttatc aataaactcg ccgatagaat acacggttat acgttcatca
     5701 tcaccaaaaa tcgcttttcc ttcaggtcgc aaattggtta aggttacccc tttatctcca
     5761 agtttaaaat tagcaaaatc ttccacattt accttaccat caatttcagt tttaagcgcc
     5821 cactttttag attgcatacg cttatatccc caataaaaaa taccacctgc agcaagcaaa
     5881 gcgcttaaca gcagccaatt accggcagtt tgcccatatt ggttgtatcc catatatacc
     5941 ccaattcctg cgacaattgc acccaaaatg gaaaaaacgg aaaatccggg aacaaagaaa
     6001 aattcgacaa aaatgaagat aacgccaaca agaattatgg tgatgattaa agctaaactc
     6061 atggttttaa tttcacggct aaattaaaga aaaaattgct gcccgcaggt tttattgcag
     6121 gataactgaa acagtgggta aatcaggtac aaatatgcaa tgcggaattt aattcagcat
     6181 ggtagtatag taagtaaaat ttttaggtga agatttatat atacctacgg cctcatccca
     6241 ataagatata cctacatttt ttatcacaat tttgtatcgg ttttcaggtt cgtaatcata
     6301 ataagtagt
//
LOCUS       DAORKW010000173         6294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_442_length_6294_cov_4.952877, whole genome shotgun sequence.
ACCESSION   DAORKW010000173 DAORKW010000000
VERSION     DAORKW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_442_length_6294_cov_4.952877"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1613
                     /locus_tag="PLB46_08195"
     CDS             <1..1613
                     /locus_tag="PLB46_08195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRF76537.1"
                     /translation="LENPNVFMSSFNRPNLYYEIRPKRSKEFAIKQIVKYIKENPRKS
                     GIVYCLNRKSTEDIAKVLNINGIRAGAYHAGMDAQTRPEVQDKFLMEDYQVIVATIAF
                     GMGIDKPDVRFVIHFDIPKSIENYYQETGRAGRDGLEGRCIAFYSYKDIEKLEKFMRD
                     KPLSEREMGAQLLAETAAYAETSVCRRRFLLFYFGEEYDRDNCGCCDNCLKPKEKIEG
                     RDYVQNALRVVDFVKENHHIPYLVDLLTGKKTTENQNYNHDKIELFGVGKEKDDHFWN
                     SILRQCLLNEFILKDIEQYGVIKLTEKGREFIKKPKSVMISLNHDYEADTSDVDMENN
                     ASKSVLDPVLMDMLTKLRDKIAKDAKMPPYVIYSENSLEEMATMYPTTMDEIVKISGV
                     SKGKADKYGKQFIDLIAQYVEENEIEKPSEFVMKQVVNKSAHKVKIIQFIDKKMPLPD
                     IAKNIGLNFPDFIDEIETIVTSGTKLSLNYYLNDLIEQDLQEEVHDYFLEMASPDLKT
                     AFNDLKDEGLSFEELRLLHIKFMSEMAN"
     gene            1656..2411
                     /locus_tag="PLB46_08200"
     CDS             1656..2411
                     /locus_tag="PLB46_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRF76538.1"
                     /translation="MQEQQTFEIKLPQFEGPFDLLLFFIERDELDIHDIPISKITKDF
                     LDYIHSMTQLNIEVASDFILVAATLMRIKAKMLLPRKELDEFGNEIDPRKELVDRLLE
                     YKRYKEVITDLSTLEEDRQLKIQRGNITLEHDLLAKKHSNEMELSSINLFVLLKVFQK
                     VMDRFNNQPTVKEHVVFSYNYSIEEESFGVYSYCKGKKNVPFAEVFTSCKDRYQAVFR
                     FLSVLDLIAQQKLTLLVGEGPNNFWLTSDQENG"
     gene            2404..3504
                     /locus_tag="PLB46_08205"
     CDS             2404..3504
                     /locus_tag="PLB46_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRF76539.1"
                     /translation="MADHPLHTDLPEIPEKEIKQQFSYKRIAVIILLSLALSAYLIYT
                     SFNFDALRGIKWDAESLLWIAAGFIFLIIRHLGYMYRLRLITDKKLNWRQSFNIISLW
                     EFSSALTPSTVGGAAVAIYFMKKEKLSLGKSAATSMLTIFLDQIFLAVFGILMWFIVG
                     GNKMFASDANCRSQSDLPLMNMFHNMQAVYFLGFGIFLIVIAGIAYGLFVNAHAFKSM
                     LNSIFSLRFLKKWQKDAVQTGNDIILTSEELKTKNVGFWAKSFLSTTITWIAFFSIPI
                     CIIHAFFDPTFADLPVIFSRMFAIWMLMLLPISPGGAGIAELTFSAMMCDFTDPALVA
                     TLALLWRIISFYPYLFTGVIILPRWIKKVYSN"
     gene            complement(3729..5114)
                     /locus_tag="PLB46_08210"
     CDS             complement(3729..5114)
                     /locus_tag="PLB46_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF76540.1"
                     /translation="MLTNHVNPIALLISMICLFQSAFSQKISREEAQADIIFLQEALT
                     NGHPGSKKEITQTVQALEGFKNSITDSIDLRPFFRNVRYCVTQLNCVHTAVDKIDRPL
                     NSLPLNLLPFDFAIDEGKLFLIGGDSTYTINYPVEVVSVNKTPSSQLVDMLWHYRSSD
                     GNLITTNNSYVPEYGYYFLASLLGFPDTFSIQLAGKTKPIIAPAVLKKQTTPPVVSIT
                     SIYAFEDCRFYRSLEDSSVYILDIDGFANHRFRPFYKDVFNYLAKNNITNLVIDLRDN
                     LGGNRNNVSNLLGYFLDSTQHYQLIKTNGAASQYADFKIIFLQFLRFNIAEFYRCKYN
                     NGQATFTYNMQPHKQQYRGKIYVLINGKSASASGITAGYLNQYCQAVLIGEEAAGGNN
                     GNNGGSYARLILPNSKVNIRFPVFHLKHDYATSDDTGGVLPDITTTYSLQEIIQQVDK
                     EMGVVYELVGD"
     gene            complement(5154..>6294)
                     /gene="kbl"
                     /locus_tag="PLB46_08215"
     CDS             complement(5154..>6294)
                     /gene="kbl"
                     /locus_tag="PLB46_08215"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRF76541.1"
                     /translation="DSGLYKNERVITSPQGADITLADGRSVINFCANNYLGLSSHPKV
                     IEAAKKAIESHGYGMSSVRFICGTQDIHKTLESKIAEFLGTEDTILYAAAFDANGGVF
                     EPLFNEEDAIISDALNHASIIDGVRLCKAVRYRYAHNDMADLEEQLKLSQAQRNRIIV
                     TDGAFSMDGTIAALDKIVELAEKYNALIMVDECHASGFLGKTGRGTHEHRGVMGKIDI
                     ITGTLGKALGGASGGFTSGRKEIIEMLRQRSRPYLFSNTLAPSITGASIAVLDLLSET
                     TELRDKLEYNTKYFREKMTAAGFDIKPGEHPIVPIMLYDAVLSQTMAAQLLEEGIYVI
                     GFYFPVVPKGQARIRVQISAGHDQHHLDKAIAAFTKVGKALKVIA"
BASE COUNT         2013 a         1128 c         1113 g         2040 t
ORIGIN      
        1 gactcgaaaa tccgaatgtg ttcatgtcga gcttcaacag acctaacctc tattacgaaa
       61 tacgtccaaa acgtagcaaa gagttcgcta tcaaacagat agtgaagtat attaaagaaa
      121 acccaagaaa atcagggatt gtatattgtc ttaaccgtaa gtcgaccgaa gatattgcta
      181 aagtgctgaa tatcaacgga atacgagcag gcgcttacca tgcaggtatg gatgctcaaa
      241 cgcgtccgga agtgcaggat aagtttttga tggaagatta tcaggttatt gtggctacca
      301 tcgcctttgg aatgggtata gacaaaccgg atgtacgttt tgtcattcac ttcgacattc
      361 ctaaatcaat cgaaaattac taccaggaaa ccggaagagc cggtcgcgat ggcctggaag
      421 gcagatgtat tgctttctat agttataagg acatcgaaaa gctggaaaaa ttcatgcgcg
      481 ataaaccact ttccgagcgc gaaatgggtg cacagttgtt agcagaaact gcagcgtatg
      541 ccgaaaccag tgtttgtcgc cgcaggttcc tattatttta ttttggggaa gaatatgacc
      601 gcgataactg tggatgttgc gacaactgtt tgaaacctaa agaaaaaatt gaaggcagag
      661 attatgttca aaatgcactt cgtgttgtag attttgtaaa agaaaatcat catattcctt
      721 atttggtaga tttacttacc ggtaaaaaaa caaccgaaaa ccaaaattat aatcacgaca
      781 aaatcgaatt atttggtgta ggaaaagaaa aagatgacca tttctggaat tctattttac
      841 gtcaatgttt attaaatgaa tttatcttaa aagatatcga acaatacggt gtaattaaac
      901 tcaccgaaaa aggtcgcgag tttattaaaa agccgaagtc ggtgatgatt tcattaaatc
      961 acgactacga agctgatacc agtgatgtcg acatggaaaa taatgccagc aaaagtgttt
     1021 tggaccctgt gttgatggat atgcttacca aattgcgtga taaaattgca aaagatgcta
     1081 aaatgcctcc ttatgtaatt tattccgaaa attcacttga agaaatggca accatgtatc
     1141 caactacaat ggatgaaatt gttaaaattt cgggagttag taaaggaaag gcagataaat
     1201 atggtaaaca gtttattgat ttaattgcac aatatgtaga agaaaacgaa atcgaaaaac
     1261 cttccgaatt cgtgatgaaa caggttgtca ataaatcggc acataaagta aaaattattc
     1321 agtttatcga taaaaaaatg ccgttacctg atattgcaaa aaatatcgga ttaaatttcc
     1381 cggattttat tgatgaaatt gaaaccattg ttacttcagg caccaaactc agtttaaatt
     1441 attatctcaa cgatttaatt gaacaggatt tacaggaaga agtacatgat tatttcctcg
     1501 aaatggccag ccccgacctt aaaaccgcat ttaacgattt aaaagatgaa ggcttgagtt
     1561 ttgaagaatt acgattactt catattaagt ttatgtccga aatggccaat taattattga
     1621 ataactactg atttatagaa gattagaaat aagcgatgca ggaacaacaa acatttgaaa
     1681 taaagctacc tcagtttgag gggccattcg atttgctgtt attttttatt gaaagagatg
     1741 aattggatat tcatgatatt ccgatttcta aaatcactaa agattttctc gattatattc
     1801 attcaatgac gcaacttaat attgaagttg ccagcgattt catacttgtt gccgctactt
     1861 taatgcgtat taaggcaaaa atgttgctgc ctcgtaaaga gttggatgaa tttggtaacg
     1921 aaattgaccc tcgtaaagaa cttgttgacc gattattaga atataaacgt tacaaagaag
     1981 ttattaccga cctcagcacg cttgaagaag atcgtcaact gaaaatccaa cgcggaaata
     2041 ttacacttga acatgatttg ctggctaaaa aacacagcaa cgaaatggaa ctgagcagta
     2101 tcaatttatt tgtgctgcta aaagttttcc aaaaggtaat ggacaggttt aataaccagc
     2161 ctaccgttaa agaacatgtt gttttttctt ataattattc tattgaggaa gaaagttttg
     2221 gcgtgtattc ttattgtaaa ggcaaaaaaa atgtaccatt tgctgaagta ttcacatcct
     2281 gcaaagacag atatcaggca gtgttccgtt tccttagcgt tttagactta attgcgcagc
     2341 agaaattaac attattagta ggtgaagggc caaataattt ttggttaact tcggatcagg
     2401 aaaatggctg atcacccact tcataccgac ttacccgaaa ttccggaaaa ggaaatcaaa
     2461 cagcaatttt catataagag aattgctgtt attattttac taagtcttgc actttctgcc
     2521 tatctgattt acacatcatt taacttcgat gcacttcgtg gtataaaatg ggatgctgaa
     2581 tcacttttat ggattgctgc cggatttatt tttttaatta ttcgtcactt gggttatatg
     2641 taccgtttgc ggttaattac tgataaaaaa ttaaactggc gacaatcttt taatattatt
     2701 tctctttggg agttttcttc tgctttaaca ccttctacgg ttggtggagc agcggtggct
     2761 atttatttta tgaaaaaaga aaaattgagt ctcggaaaaa gtgctgcaac cagcatgctg
     2821 actatttttc tcgaccagat atttcttgct gtttttggaa ttttaatgtg gtttattgtt
     2881 ggcggaaata aaatgtttgc atcagatgct aattgtcgta gtcaaagtga cctcccactt
     2941 atgaatatgt ttcacaatat gcaggcagtt tattttttag gatttggtat tttcttaatt
     3001 gttatagccg gcatagcata cggattattt gtaaatgcac atgcatttaa atcaatgttg
     3061 aattcaattt tcagtttgcg gtttttgaaa aaatggcaaa aagatgcagt tcaaaccggt
     3121 aatgatatta tacttacttc tgaagaactt aaaacaaaaa atgtaggctt ctgggcaaaa
     3181 tcgtttttat cgacaactat tacttggatt gcattttttt ctatcccaat ttgtatcatt
     3241 catgcatttt ttgatccgac ttttgctgat ttacctgtaa tatttagtcg catgtttgcc
     3301 atttggatgt tgatgttatt acccatctca cccggtgggg ctggtattgc ggaattaact
     3361 ttcagcgcca tgatgtgcga tttcaccgac cctgcattag tagccacttt agcattgctg
     3421 tggcgcatca tttcatttta tccgtattta tttaccggcg taataatatt acctcgctgg
     3481 attaaaaagg tttatagtaa ttaatattac aggatataaa acgaatttat atacattgac
     3541 agattgcaat ggtcaatacg tgtgttgacg gttttatcgc gttgaatttg aaatattaaa
     3601 attgatagtc aacacgtggg ttgacggcta caaggcttcc gaatttcatt ccatctctaa
     3661 accggctgtt aatcagcaca tcaacacatc agcacattaa catccacccc ggcggacaca
     3721 ttatcacatt aatccccaac caattcataa accaccccca tttctttatc cacctgttgt
     3781 attatttctt gcaatgaata tgttgtagtg atatcaggta atacaccacc cgtatcatcg
     3841 cttgttgcgt aatcgtgttt taaatgaaaa accgggaaac gaatatttac tttcgaattt
     3901 ggtaaaatta agcgcgcata acttccacca ttattaccat tatttccccc tgccgcttct
     3961 tctccaatta aaacggcctg gcaatattga tttaaatacc cggcagtaat cccacttgca
     4021 gatgctgatt taccatttat tagcacatat attttacccc tatattgctg tttgtgtggt
     4081 tgcatgttat aggtaaaagt agcctgacca ttattatact tacatcgata aaattccgca
     4141 atattaaatc gtaaaaattg taagaaaatt attttaaaat ccgcatattg acttgcagcc
     4201 ccatttgttt ttatgagttg ataatgttga gtgctatcta aaaaataccc taacaaatta
     4261 ctaacattgt tgcggtttcc tcctaaatta tccctcaaat caattaccag atttgtgata
     4321 ttatttttcg ctaaataatt aaacacatct ttgtaaaagg gcctgaacct gtgattggcg
     4381 aatccgtcta tatctaaaat ataaacagaa ctatcttcca ggctgcgata aaacctgcaa
     4441 tcttcaaagg cataaattga agtaatacta actacaggag gagtggtttg tttttttaat
     4501 accgccggag caattatagg ttttgttttg ccggcaagtt gaatggaaaa cgtatccgga
     4561 aaacctaata agctggctaa aaaataataa ccatattccg gaacataact gttatttgta
     4621 gtaattaaat taccatcaga actacggtaa tgccaaagca tatcaaccaa ttgtgatgat
     4681 ggtgttttat taacagatac aacttctaca ggataattta tcgtataagt gctatcacca
     4741 ccaattaaaa ataatttacc ttcatcaatg gcaaagtcaa atggcaataa attgagtggt
     4801 aaagaattaa gggggcgatc tattttatca actgccgtat gcacacaatt taattgtgta
     4861 acacaatatc gaacatttct aaaaaagggt cttaaatcta ttgagtcggt aatgctgttt
     4921 ttaaatccct ctagggcctg aacagtttgt gttatttctt ttttagagcc aggatggcca
     4981 ttggtgagtg cctcttgtaa aaatataata tcggcttgcg cttcttcccg ggaaattttt
     5041 tgactaaatg ctgattgaaa taaacaaatc atgctaatca ataacgcaat aggattaaca
     5101 tgatttgtaa gcataaaatc aatttatttc acgtaataat acgtggttta taattatgca
     5161 attactttta acgctttacc cacttttgta aatgctgcaa ttgctttatc taaatgatgt
     5221 tggtcgtgac ctgcagaaat ttgaacgcga attcgtgcct gaccttttgg cacaaccgga
     5281 aaataaaatc caattacata aataccttct tccaataatt gtgctgccat ggtttgactt
     5341 aatacagcat catacaacat aattggcaca ataggatgtt cgcccggttt aatatcgaaa
     5401 ccagcggcag tcattttttc gcggaaatat tttgtattgt attcgagttt gtcgcggagt
     5461 tcggttgttt cacttaataa atccaatacc gctattgatg cgccggtaat tgatggagct
     5521 aatgtatttg aaaataaata aggacgtgaa cgctgacgca acatctcaat tatttctttt
     5581 cttcctgaag taaatccacc acttgctccg cctaatgctt ttcctaatgt tccggtaatg
     5641 atatcaattt tacccattac accacgatgc tcatgtgtgc ctcttccggt ttttcctaaa
     5701 aatccggagg cgtgacattc atctaccatg attaatgcat tatatttttc agcaagctca
     5761 acaattttat ctaaagcggc aatggttcca tccatactaa atgctccatc tgttacaatt
     5821 attctattgc gttgtgcttg cgataattta agttgttctt ccaaatctgc catatcatta
     5881 tgcgcatatc tgtaacgcac cgctttgcat aaacgcactc catcaattat tgatgcgtga
     5941 tttaatgcat cagaaataat agcatcttcc tcattaaata atggttcaaa aacaccgcca
     6001 tttgcatcaa atgcagcagc atataaaatg gtatcttcgg ttcctaaaaa ttctgctatt
     6061 ttgctttcca gtgttttatg aatatcctga gttccgcaaa taaatcgcac tgaactcatt
     6121 ccgtaaccat gactttcaat tgcttttttt gctgcttcta ttacttttgg atgtgaactt
     6181 aatcctaaat aattattggc acaaaaatta atcactgacc tgccgtctgc taaagtgatg
     6241 tcagcaccct gaggtgaagt aataactctt tcgtttttat ataatcctga atct
//
LOCUS       DAORKW010000174         6291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_443_length_6291_cov_6.378768, whole genome shotgun sequence.
ACCESSION   DAORKW010000174 DAORKW010000000
VERSION     DAORKW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_443_length_6291_cov_6.378768"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..2075)
                     /locus_tag="PLB46_08220"
     CDS             complement(195..2075)
                     /locus_tag="PLB46_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF76542.1"
                     /translation="MKYCYSLLLLLFVYTSTKAQITAYPDTSICPGETVQLGTTLVDD
                     CGNCYTYQSLAYAPEPIGGEAITMVDDTYIGPYPIGFDFCFFGVEYDEFYLCSNGWIS
                     FVTPGGLWAGNWTPDGPIPDAAANVPRTAIFACWTDWNTGAISCDDCIHFETVGVTPN
                     QRLLVTWDEVPLYSDACADDLGTFQIILHETSNFIDNHFTDAMVCPTWDDGVATQGLQ
                     NQTGTIAFTVEDRNATAWEAHDESWRWYTSTAEWYDEDGVLLGVGQVIDVTPDVTTTY
                     TVVQTMCDGTTYTDDVTVEVGAAFDTEVLTTDITCGGDDNGTATVDVTGGLEPYTYEW
                     STGVTGTNTLTGLDGGTYSVIVTEAGGCQREYTFTINEPEELVANATDIIDNPCFDYE
                     EGSATIEANGGTIPYAYSLDGGATTILNTFENLPAGTYTVTVTDANGCTVDETFTIEE
                     PILLTANLTADDNSIFVGQTALITLTTSLPGITSVSWEPEVPCLDPPCFSTIVAPTTT
                     TTYEVTVIDDQGCVATDTITIFVEFVPEVFFPNAFSPNGDSFNDQFAGVSYNVTDFHM
                     SIFNRYGEMVYETNSFILGTGWDGNFEGEPQEMGTYVWQVTASFNNGDTYQGSGNVIL
                     VR"
     gene            2270..2443
                     /locus_tag="PLB46_08225"
     CDS             2270..2443
                     /locus_tag="PLB46_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76543.1"
                     /translation="MILEKVSFDMNLFRIELKKALDQLIGEEITELKKWCITRFGLNY
                     CKTAAPEIPLDLD"
     gene            complement(2698..4344)
                     /locus_tag="PLB46_08230"
     CDS             complement(2698..4344)
                     /locus_tag="PLB46_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HRF76544.1"
                     /translation="MRIFLITIVLLIIAGCANQVAPSGGPKDVTPPTVLDYEPDSASV
                     NFTSNTITIKFNEYIILNDVFNQIVISPPLNETPEYKLKGKTLTISLKDTLKPATTYT
                     INFGQAIKDNTAGNVKENFTYVFSTGSFIDSLQVSGKVIDLLTAAPATKTYVVLYENP
                     TDTSFTTSKPYYFAKTDATGNFIINNIKSGTYKLYAIEDQNFNYFYDLPNEKIAFQSS
                     TLTIDSNITNQKLFLYSENKIKQNLLEAKSTRYGQTRLVFAKNATDTKINYTGTDTAD
                     YFFTRNISNDTILFWHTNYLLDTHQLSISFDTTLIEKIIETKEFPADSNFLKSLNTFT
                     KNVITLAKGGDPNARAEWVPGKPIDYLFYNPIKTVTNTTIQILQDSIPVTDYTLQIDS
                     LDARKMRINYNWKPEKNYTIIIPEKATQDIFGLYNSADTIKLTVKKIDAFASLSTSIK
                     NTSGSPIIFQFMKFDMTIIDEFYLDTLKFENADNTYIVKQSYLTPGVYKIRAILDADK
                     NGKWTPGDLSQNRPPESVIFFPVDQNLRANWENEIEWHLK"
     gene            complement(4501..>6291)
                     /gene="mnmG"
                     /locus_tag="PLB46_08235"
     CDS             complement(4501..>6291)
                     /gene="mnmG"
                     /locus_tag="PLB46_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRF76545.1"
                     /translation="NMGAKVMLVTMNMQTIAQMSCNPAMGGVAKGQIVREIDALGGYS
                     GIVTDKSAIQFRMLNLSKGPAMWSPRAQSDRMMFAAEWRYMLEQNKNVDFWQDTVAGL
                     LIKDGRIAGVRTSLGMEIPSKAVVLTNGTFLNGLIHIGEKNFGGGRVAEKAATGITEQ
                     LISLGFESGRMKTGTPPRVDGRSLNYNLMEEQLGDEVPQKFSYTDTKPLAKQRSCWIT
                     YTSDAVHEVLRTGFDRSPMYSGRIKGPGPRYCPSIEDKIDRFASKERHQIFVEPEGWN
                     TVEVYINGFSTSLPEEVQYAALKKIQGFEDVKMFRPGYAIEYDYFPPVQLSHTLETRL
                     IKNLFFAGQINGTTGYEEAACQGLMAGINAALSVAEKAPFQIDRSEGYIGVLVDDLIN
                     KGTEEPYRMFTSRAEYRVLLRQDNADIRLTQKSHAIGLASDERLKRVNEKISATTKLI
                     DFLRNLSVDPDQVNGMLESKGSTVIKQKTKVGQLLSRPHIGLNDLIGLNSNLDTELLQ
                     YETEFREQAEISIKYEGYISKEKELVDKMLRLENIIIHDAINYHQLHSISSEAREKLS
                     KIKPKTLGQASRISGVSPSDIAVLMIHIGR"
BASE COUNT         1865 a         1215 c         1101 g         2110 t
ORIGIN      
        1 tttctttttg atgactaata attgatttag gaggaccatt tttatgtata aaccatcaag
       61 atttttctta atcgaatgaa gtgacacata aataataacc cgcaaaattc tacaaaattg
      121 cgggttaaga aaatctttga aattgttatc tataaatggt tgcgccaaaa tgcatataaa
      181 ttaaaattaa tatgttatct caccaaaatt acattaccgg aaccttgata agtatcacca
      241 ttattaaatg atgctgttac ttgccaaacg taagttccca tttcttgagg ttcaccttca
      301 aagttaccgt cccaacctgt acccagaata aatgaattag tttcataaac catttcacca
      361 tatcggttaa atatgctcat atggaaatct gttacattat aagatacccc cgcaaactga
      421 tcgttaaaac tgtcgccatt aggtgaaaaa gcatttggaa aaaatacttc cggcacaaat
      481 tctacaaaaa tggtaatggt atctgttgcc acacaaccct ggtcgtcaat cactgtaact
      541 tcataggttg tagtagttgt aggtgctact attgttgaaa aacaaggcgg atctaaacaa
      601 ggcacttccg gttcccaact tactgaagta atacccggta ggctagtggt taatgttatt
      661 aatgcggttt gtccaacaaa aatagaatta tcgtctgctg ttaaattggc agttaataat
      721 attggttctt caatagtaaa agtttcatct acagtgcatc catttgcatc tgttactgta
      781 acggtataag tgccggcagg aaggttttca aaggtgttta aaattgtagt agcacctccg
      841 tccaaactat aagcataagg gattgtacct ccgttagctt caatagtggc tgaaccttct
      901 tcataatcga aacatggatt gtctataata tcggttgcgt tggcaactag ttcttcaggt
      961 tcgtttattg taaatgtata ctctctttgg caaccacccg cttcggtaac aataacagag
     1021 taagtgcctc catcaagtcc ggttagtgta ttggttccgg taacaccggt gctccattca
     1081 taagtgtatg gttctaatcc gccggttaca tcaactgttg ctgttccgtt gtcatcaccc
     1141 ccacaagtaa tatctgtagt taaaacttct gtatcaaatg cagcacctac ttcaactgta
     1201 acatcatcgg tataagttgt tccatcacac attgtttgca ccacggtata tgttgtagtt
     1261 acatcaggcg taacatctat tacctgacct acacctaata atacaccatc ttcatcatac
     1321 cattctgcgg tactagtata ccatctccaa gattcatcat gagcttccca cgcagttgca
     1381 tttctgtctt ctacggtgaa ggcaatcgtt ccggtttgat tttgtaaccc ttgagttgca
     1441 acgccatcat cccaagttgg acaaaccata gcatcagtaa aatggttatc tataaaatta
     1501 cttgtttcat gtaaaataat ctgaaaagta cccaaatcat cggcacaagc atcactatat
     1561 aaaggaactt catcccaggt tactaataat cgttggtttg gtgttactcc aacagtttca
     1621 aaatgaatac aatcatcaca tgatattgcg cctgtattcc aatcggtcca gcaggcaaaa
     1681 atggctgtcc gaggaacatt agcggctgca tcaggaattg gaccatcagg tgtccagttt
     1741 cccgcccata atccgccagg ggtaacaaaa cttatccatc cgtttgaaca taaataaaac
     1801 tcatcatatt caactccaaa gaaacagaaa tcaaaaccaa ttggatacgg accaatataa
     1861 gtatcatcaa ccatagttat ggcttcaccg ccaataggtt ccggggcgta agctaaactt
     1921 tgataagtgt agcaattgcc gcagtcatca accagcgtcg taccgagttg aaccgtttcg
     1981 cccggacaaa tactcgtatc cggataagct gttatttgag ctttggttga agtgtaaaca
     2041 aagaggagta aaaggagtga gtaacaatac ttcataaatg cttgaaattt agattgttaa
     2101 aatcgtaata aagataagga aattttttgc tgtcttattc tgtgtataat cgtcattttt
     2161 ttgtgaaaat gacctatttt gttaaaaaat caaattttgc aacatttgta aattatactc
     2221 gttttaatca tataatttca aagaaatgac gatgcttgaa tattgcaaaa tgatccttga
     2281 gaaggtgagt tttgatatga acctttttag aattgaatta aaaaaagcgc ttgaccaatt
     2341 aattggtgaa gaaatcaccg agttaaaaaa atggtgcatc actcgttttg gattaaatta
     2401 ttgtaaaacg gctgctccgg aaattccgct cgatttagat taagcttttt atatttttga
     2461 ttcgcccgac agcaagaaat gttcaggaaa gcaactcggt aatatcatct aactaatttt
     2521 ataatatttc gagttaattg gacacctttg cgcgttattt cgcctttatt tttaatttga
     2581 gcgacgacaa ttgtgtttga tactatgtgt cgaaaaagtt aaaatctttc aatacaagcc
     2641 aattctcgac ttcgtttttt tcgctgattt caattttttg cgacattcaa aattggctta
     2701 ttttaaatgc cattctattt cattttccca gtttgcgcgt aagttttgat ctaccggaaa
     2761 gaaaattacg gattccggag ggcggttttg tgacaaatct ccgggtgtcc atttgccatt
     2821 tttatctgca tcaagaatgg ctcgaatttt ataaacgcca ggggttaagt atgattgttt
     2881 aactatatat gtgttatctg cgttttcgaa ttttaaagta tctaaataaa attcatctat
     2941 tattgtcata tcaaatttca tgaattgaaa aatgattgga cttccgcttg tgttttttat
     3001 ggaagtagaa agggaagcaa aagcatctat ttttttcact gttagtttga tagtatcagc
     3061 gctattgtat aacccgaaaa tatcctgagt tgctttttcg ggaattatta tggtataatt
     3121 tttttcaggt ttccaattgt aattgatacg catttttcgt gcatccagag aatcaatttg
     3181 taaggtataa tctgtcaccg gaatggaatc ctgcaatatt tgtatggtag tattggtaac
     3241 ggttttaata ggattgtaaa aaagataatc aattggtttt cccggaaccc attcggctct
     3301 ggcatttggg tcaccgcctt ttgcaagcgt aattacattt ttagtgaatg tatttaatga
     3361 cttcaaaaaa ttggaatcag caggaaactc ttttgtttca ataatttttt caattaacgt
     3421 agtatcaaaa ctgatactta attgatgtgt gtctaataaa taattggtat gccaaaaaag
     3481 aatagtatcg ttacttatgt tcctggtaaa aaagtaatca gcagtatcag taccggtata
     3541 attaattttg gtatccgttg cattttttgc gaacactaaa cgtgtttgtc cataccgtgt
     3601 actttttgcc tcaagtaagt tttgttttat tttattttct gaatatagga agagtttttg
     3661 attggtgata ttactgtcta tagttagggt actggattga aatgcaattt tttcgttggg
     3721 taaatcgtag aagtaattaa aattttgatc ctctattgca tataatttat acgtacctga
     3781 tttgatattg ttgatgataa aatttccagt cgcatctgtt tttgcaaaat aatagggttt
     3841 ggaagtagtg aacgaggtat cagttggatt ttcatataat accacataag tttttgttgc
     3901 aggtgcagct gtaagtaaat caatcacttt acctgaaact tgtagtgagt caataaatga
     3961 accggttgaa aatacatacg tgaaattttc cttcacattg cccgcagtat tgtcttttat
     4021 tgcctgtccg aaattgatgg tataagtggt tgccggtttt aatgtatcct ttaatgatat
     4081 ggttaacgtt ttgcctttta atttatactc gggtgtttcg tttaaagggg gagagatgac
     4141 tatttgatta aatacatcat ttaatataat gtattcgtta aactttatgg tgatggtatt
     4201 tgatgtaaaa ttgacagatg ctgaatccgg ttcataatct aaaaccgtcg gcggcgtcac
     4261 atcttttggt ccgcctgatg gtgcaacctg attagcacaa cctgctatta ttaataatac
     4321 tatagtaata aggaaaattc gcattggtgt tgcaaagata gaaattcact taacggctat
     4381 aacgccaaag cgattgtttg ttcaaaaatc ttttacttaa ataataatga aacgaatttg
     4441 atattattta agaaaaagtg atgtaagtaa ttgaaataca gggagttata acaaaacatt
     4501 tcaacgaccg atatgaatca tcaaaacagc aatatcactt ggagaaacac cgcttattct
     4561 acttgcttgt ccaagcgttt tcggttttat tttactgagt ttttctctcg cttcagatga
     4621 tatggaatgt aattgatgat agtttatagc atcatgaatg atgatatttt ctagacgcaa
     4681 cattttatct acgagttctt tttctttgga gatgtaccct tcatatttaa ttgaaatctc
     4741 agcttgctct ctaaattccg tttcgtattg aagtagctct gtatcgagat tactatttaa
     4801 tcctattaga tcgttcagac caatatgtgg tctgctaagt aactggccaa ctttagtttt
     4861 ttgtttaatt actgttgaac cttttgattc aagcatccca tttacctggt ccggatctac
     4921 cgaaagatta cgcaagaaat ctatcagttt agtagtagct gaaatttttt cattcactcg
     4981 ttttaagcgc tcatccgatg ccaatccaat ggcatgcgac ttttgagtaa ggcgaatatc
     5041 tgcattatct tgtcgcaaca atactctata ctccgccctg gaggtaaaca ttctatatgg
     5101 ttcttcagta cctttattaa ttaagtcatc aaccaaaacc cctatatacc cttcacttct
     5161 atctatctgg aatggagctt tttcagctac agataaagct gcattaatac cggccattaa
     5221 cccctgacaa gcagcttctt catacccggt tgttccatta atctggcctg cgaagaatag
     5281 gttttttatc aaccttgtct ccaaagtatg gctcaattgc accggtggga agtagtcgta
     5341 ttctatagca taacccggcc tgaacatttt cacatcttca aatccctgaa tttttttcaa
     5401 tgctgcatat tgcacttctt ccggtaagct ggtagaaaac ccattaatat atacctctac
     5461 ggtattccaa ccttcgggct ctacaaatat ctggtggcgt tcttttgaag caaagcggtc
     5521 tattttatct tctatactag gacaatatct tggacccggt cctttaatcc tgcctgaata
     5581 cataggagac ctatcgaaac cggtccttaa aacctcatgc acagcatcag aagtataggt
     5641 aatccagcag cttctttgtt tagccagagg tttagtatcg gtataagaaa acttctgagg
     5701 aacttcatct cctagttgtt cctccatgag gttgtaattc aaacttctgc catcaaccct
     5761 tgggggggtt ccggttttca ttcttccact ttcaaatccc agacttataa gttgttcagt
     5821 aataccggtc gcagcctttt ctgcaacacg accaccacca aagttttttt ctcctatatg
     5881 tattaaacca tttaagaatg ttccattggt aagaactacc gccttagatg gtatttccat
     5941 tcccaggctt gtccgcaccc ccgcaattct tccatcctta atcagcaaac ccgccacagt
     6001 atcctgccaa aaatctacat ttttgttttg ttccagcatg tatctccatt cggcggcaaa
     6061 catcattcta tcgctttgag cccttggact ccacatagca ggtcctttac tcaggttcaa
     6121 catcctgaac tgaatagcac ttttatcggt cacaatcccc gaatagccac ccaaagcatc
     6181 gatctccctc actatctgac ctttcgccac acctcccata gctgggttgc agctcatttg
     6241 ggcaatagtc tgcatgttca tggtcacaag catcaccttc gcccccatat t
//
LOCUS       DAORKW010000175         6285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_445_length_6285_cov_4.826003, whole genome shotgun sequence.
ACCESSION   DAORKW010000175 DAORKW010000000
VERSION     DAORKW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_445_length_6285_cov_4.826003"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="PLB46_08240"
     CDS             complement(<1..1563)
                     /locus_tag="PLB46_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF76546.1"
                     /translation="MSELMQNNNITFEAFKQDVINDFKMACLSREVSLLGRKEVLTGK
                     AKFGIFGDGKEVAQIAMAKVFKAGDFRSGYYRDQTFMFSSGMASPEHFFAQLYAIPDT
                     IIEPNSGGRQMNGHFATRLLDNNGNWNDQTATKNTSSDTSPTASQMVRAVGLALASKL
                     YRQNPETDSRGFSVNGNEVVFATIGDASTSEGVFWEAVNAAGVLKIPLAFFVWDDGYG
                     ISVPQKYQTTKQNISEVLSGFQLDEEGNGVRIYEVKGWDYAALVQTFEEGIELCRREH
                     VPVFFHVTEVTQPQGHSTSGSHERYKSKERLEWEAEWDCIQKMKTWMIASSIATDEQI
                     QGMLAEAKEIAKAEQKAAWNKHLLSIKADMDVLFSLLTGLESNTNNTSGVNQAISTLR
                     STIDPVRRDVMKAAKHALYATAGETSGERTALLDWVKQYDQLNDVRYNGNLWSNSDDA
                     VIKAAVIQPEYATDAPLVNGFEIINKCFDEHFAKNDKIFAFGEDLGKIGDVNQGFAGL
                     QEKYGENRIFDVG"
     gene            1758..2243
                     /locus_tag="PLB46_08245"
     CDS             1758..2243
                     /locus_tag="PLB46_08245"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRF76547.1"
                     /translation="MKKLFYTLPLIMLIATASFAQTSVSPSTGSTTATPVINPDAGEF
                     QWAEETHDFGTIPQGVPVKNKFLFTNTGKSPIIISNVQKTCGCTVTDWTKEPIMPGQQ
                     GFVMAEFNAAKEGPFTKAITVQSDSKTPSVKLYFKGTVLKSEQAGSVPEQNNIFNQGG
                     N"
     gene            2264..2704
                     /locus_tag="PLB46_08250"
     CDS             2264..2704
                     /locus_tag="PLB46_08250"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRF76548.1"
                     /translation="MKKILSIIAFVAVTGIVSAQTASSVSNPNAAAFEWVSDTYDFGK
                     IPQGIPATAKYEFINTGKEALIITDVQKTCGCTNTEWTKEPIAPGQKGYISAVYNAAN
                     EGGFTKSITVLSNASTPSVKLTFKGTVIKDDAGSVPAQETIFGN"
     gene            2895..4094
                     /locus_tag="PLB46_08255"
     CDS             2895..4094
                     /locus_tag="PLB46_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRF76549.1"
                     /translation="MPKISQRGNQMPASPIRKLVPFAEKAKLRGIKIYHLNIGQPDIA
                     TPTQMMDAVKHTDLKVLEYSHSAGFESYRRKLVQYYKSRNIDINHHQIMITTGGSEAI
                     MFGMMSCLDAGDEVIIPEPLYANYNGFSTAGNIVIKPITSHIENGFALPPVSEFEKMI
                     TPKTKAILICNPNNPTGYLYSKEELMQLRDIIIKYDLFLFADEVYREFVYDGKEHFSV
                     MNLDGLDEHVILMDSISKRYSACGARIGALISRNNKVMETAMKFAQARLSPPTLEQVL
                     AEAFLDVPESYFTEVEKEYVERRNLCVQRLNAMDGVLCPTPGGAFYAIARLPIDDADV
                     FCQWLLESFNLNNETVMLAPATGFYSTPGLGKNEVRIAYVLNKNDINKAMDCLDAALK
                     EYPGRTA"
     gene            4218..5615
                     /locus_tag="PLB46_08260"
     CDS             4218..5615
                     /locus_tag="PLB46_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF76550.1"
                     /translation="MKKLLLLIGICIINAFAFGQEINGAWLGKLNVGVELRVVFNING
                     SNDSLNSTLDSPDQGAFGIPSSSTTYINGELNIAVPAINGGYKGTYNAEKKQFEGLWL
                     QGPNKIPLALVKVDSVPALNRPQTPKLPYPYQSENITFLNNEAPGVELAGTLTIPEGK
                     GPFNAVVLVSGSGPQNRNENLLNHEPFLVLSDYLTRNGIVVLRYDDRGVGESKGDFNA
                     STSMDFASDASAAVNYLVGRTDIPIRNIGIAGHSEGGLIAPITASENKEVDFIVLMAG
                     PGIPGDSILLLQGDLIAKASGISDESIKALHELRKNIMQVAKDEIDNDVMRNNMITLN
                     KNFIANTPKHILDELEMTEDDVEEGLDIYIGEWMQFFLAYNPRPTLEHTTIPVLAING
                     TTDLQVPYKENLEAIEKALTKAGNKNYKILPLQNLNHLFQQSLSGNPAEYGTIEETFN
                     EQAMSIIANWILSLK"
     gene            5776..6264
                     /locus_tag="PLB46_08265"
     CDS             5776..6264
                     /locus_tag="PLB46_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76551.1"
                     /translation="MEIEIITVAGLTIGEPVTSLTDIVLAVISFTLFARIKNRLNESF
                     FNNSWRMFFLFMGFSTGTGAIAHALNGSDAIRWYNTLFMAMTVFSSISVFFALKATIR
                     FTKMLPSIRTVLTIANYTLLAVFTVYTVITNNFEIFKIHAAAGLFLIFITHLVASSCA
                     RL"
BASE COUNT         1940 a         1295 c         1169 g         1881 t
ORIGIN      
        1 acccacatca aaaatccggt tttcgccata tttttcctgt aagccggcaa atccctgatt
       61 tacatctcca attttaccca aatcttcacc aaaagcgaat attttatcat ttttagcaaa
      121 atgctcgtcg aaacacttat taataatctc aaaaccgtta acaagtggag cgtcggtagc
      181 gtattcaggt tgaatcacag ccgcttttat cacggcatca tctgaattac tccataaatt
      241 tccattgtat cgcacatcgt taagctggtc gtattgcttt acccaatcta atagagcagt
      301 cctttcacca cttgtttcgc cggcagtagc atacaatgca tgtttagccg ctttcatcac
      361 atcacgacgc acagggtcaa ttgtcgacct taatgttgat attgcttggt taactcctga
      421 agtattgttg gtattggatt ccagccctgt tagtagagaa aacaatacat ccatgtctgc
      481 cttaatagac aaaaggtgtt tattccaagc cgctttttgt tctgctttag ctatttcttt
      541 agcctcagct aacataccct gaatttgctc atctgtagct atgcttgacg caatcatcca
      601 ggttttcatt ttttgaatac aatcccattc cgcttcccat tccagtcttt ccttagattt
      661 ataacgttca tgagaacctg aagtggagtg accttgtggt tgtgttacct ctgttacgtg
      721 aaaaaacaca ggtacatgtt cgcgacggca aagttcaatg ccttcctcaa atgtttgaac
      781 cagggcagca taatcccagc ctttcacttc ataaattctc accccattgc cttcttcatc
      841 caactggaaa ccggataaca cttctgaaat attctgtttg gtagtctgat atttttgcgg
      901 aacagaaatg ccataaccat cgtcccaaac aaaaaacgca agtggaattt ttaacacccc
      961 tgcagcattc actgcttccc aaaacacgcc ttcagaagtg ctggcatcac cgatagtagc
     1021 gaaaaccacc tcatttccat ttaccgaaaa tcctcggcta tctgtttccg gattttgtct
     1081 gtataatttc gatgctaaag ccaaacccac agcgcgcacc atttgtgatg ccgtaggaga
     1141 tgtatctgaa gaagtatttt tagtagcggt ttggtcgttc caattgccat tattgtcgag
     1201 taaacgtgtc gcaaaatggc cattcatctg ccttccaccc gaattcggtt ctataattgt
     1261 atcaggaata gcgtacaact gcgcaaaaaa atgttcaggt gaagccattc cggagctaaa
     1321 cataaaagtc tggtcgcgat aataccccga cctaaagtcg ccggccttaa aaacctttgc
     1381 catggcaatt tgagcaacct ctttgccatc gccaaaaatg ccaaacttgg cctttccggt
     1441 taacacctct ttacggccta aaaggcttac ctcacggctc aaacaagcca ttttgaagtc
     1501 gttgataacg tcctgcttaa aggcctcaaa agtgatatta ttattttgca ttaattccga
     1561 catattatta aagaataagg ctgcaaaaat acataaataa gcctggtctc cggttgcata
     1621 aatgggtatt ttgaaaaaaa attgcggtac aacggctact ggtataattc ttgtatgttt
     1681 tctgcggatt caaacctttt aacaagattt gatattttta catcagtaag cattaacaac
     1741 taaaattgta ttatattatg aaaaaattgt tctacacact cccgttgatc atgttgatcg
     1801 ccaccgcatc gtttgcgcaa acttcagttt ctccttctac aggttcaaca accgcaactc
     1861 cggttattaa tcctgatgca ggtgaattcc agtgggctga agaaacgcat gattttggaa
     1921 ctatacctca gggtgttccg gttaaaaaca aatttttgtt taccaatacc ggtaaatcac
     1981 ctattattat atcaaacgtt caaaaaactt gtggttgcac agtgactgac tggactaaag
     2041 aacctattat gcctggtcaa caaggttttg taatggctga atttaatgct gctaaagaag
     2101 gacctttcac caaagcaatc actgttcagt ctgattcaaa aacaccttca gtgaaattat
     2161 atttcaaagg aacagtgtta aaatctgagc aagcaggtag tgttcctgaa caaaacaata
     2221 tttttaatca gggaggcaat taatctcaat aaactacaag tcaatgaaaa aaatattatc
     2281 tataatcgct tttgtagccg taacaggaat tgtttcagca caaacagcca gcagcgtatc
     2341 taatccgaat gcagcggcat ttgaatgggt gtctgatact tacgattttg gtaaaatccc
     2401 gcaaggtata cctgcaacag caaagtatga atttatcaat accggtaaag aagcccttat
     2461 tattactgat gttcaaaaaa catgtggttg tactaatact gaatggacta aagaaccaat
     2521 tgctccggga caaaaaggat atatttctgc agtgtataat gcagcgaacg aaggtggatt
     2581 tactaaatct attaccgttt tatcgaatgc aagcacacca agcgttaaat tgacttttaa
     2641 aggcactgta ataaaagatg atgcaggaag tgttcctgct caggaaacga ttttcggaaa
     2701 ttaatacttt aacgaaatct gcgacaactc cgcaagtctt gaagacttgc ggagtttttt
     2761 ttgatggtag ctataggtag ttttcgacac ctgacaggtc ttaaaacctg acaggtatag
     2821 ttgtattagt ctactatcta ttgccaactt ttgggtgtat tgacacatcg acacattata
     2881 cttacctttg ctccatgccg aaaatatcac aacgcggaaa ccaaatgccc gcatcgccaa
     2941 tcagaaaatt agtgcccttt gcagaaaaag ctaaactcag aggaattaaa atatatcatt
     3001 tgaatatcgg acagccggat attgctaccc ctactcaaat gatggacgct gttaaacaca
     3061 ccgatttaaa agttttagaa tattcccatt ctgctggttt tgaatcctat cgccgtaaat
     3121 tagttcagta ttataaaagc cgcaacattg atatcaacca ccatcaaatt atgattacca
     3181 caggtggctc cgaagctatt atgtttggta tgatgagttg tctcgatgcc ggtgatgagg
     3241 tgattattcc tgaaccattg tatgctaatt ataacggatt ttctactgca ggaaatattg
     3301 ttattaaacc cattacttct catatcgaaa atggttttgc attgccaccg gtttcggaat
     3361 ttgaaaaaat gattaccccg aaaacaaagg caattttaat ttgtaatccc aacaacccta
     3421 ccggatatct ctactcaaaa gaagaattga tgcaattgag agatatcatt attaaatacg
     3481 atttattcct gtttgccgat gaagtatatc gcgaatttgt ttacgacgga aaagaacatt
     3541 tttctgtaat gaatttagat ggcctcgacg aacatgtcat tttaatggat tccatttcca
     3601 aaagatatag cgcatgtggt gcccgtattg gtgcattaat ttcccgcaat aataaagtaa
     3661 tggaaaccgc aatgaaattt gcgcaagcac gtttatctcc accaacgttg gaacaagtac
     3721 ttgccgaagc atttctcgat gttcctgaat cgtattttac ggaagtagaa aaagaatatg
     3781 tggaacgtcg caatttatgt gtgcaacgat taaatgcaat ggacggcgtg ttatgcccaa
     3841 ctcctggtgg cgctttttat gccatagcgc gactaccaat cgatgatgct gacgtatttt
     3901 gtcaatggtt gcttgaatca ttcaacctca acaatgaaac ggtaatgctt gctcctgcaa
     3961 ccggatttta ttctactccg ggtttaggaa aaaatgaagt gcgtattgca tatgtgttga
     4021 ataaaaatga tataaataaa gcaatggatt gtctcgatgc tgcactcaaa gagtatccgg
     4081 gcagaactgc ttaatgttat cattttattt atcagcataa ccgatatttc cggtttcgtc
     4141 tgtcaattat ctgcgttcag ctaaaaaatt tgaaccagct tttttgtgca tgtacatttg
     4201 taaaaaaaat cggtgttatg aaaaaacttt tgctactaat aggtatctgc attattaatg
     4261 ccttcgcttt tggacaggaa ataaacggag catggttagg aaaattaaat gttggtgttg
     4321 aattacgcgt ggtatttaat ataaatggtt caaacgattc acttaatagt acattagata
     4381 gtccggacca aggtgctttc ggtattccgt cctcatcaac aacctatata aatggtgaac
     4441 ttaatattgc tgttccggcc attaatggtg gttacaaagg cacctataat gcagagaaaa
     4501 aacaattcga aggattatgg ctacagggac caaataaaat tcctttagcc cttgtaaaag
     4561 ttgattctgt ccctgcgcta aatcgtcctc aaacacctaa actaccttat ccatatcaat
     4621 cagaaaatat tacattcctt aacaacgaag cacctggtgt tgaacttgca ggaacactta
     4681 ccattcccga aggtaaaggc ccgtttaatg cggttgtttt ggtttcagga agtggccctc
     4741 aaaacaggaa tgaaaattta ttaaatcacg aaccattttt agtgttgagc gattacctca
     4801 cacgaaatgg tatagttgtg ttgcgttacg acgatcgtgg tgttggcgaa tcaaaaggcg
     4861 attttaatgc ttcaacgtcg atggattttg ccagtgatgc aagtgctgcg gtaaattatt
     4921 tagtaggaag aaccgatatt cctattcgta atattggcat tgctggtcac agcgaaggtg
     4981 gattaattgc accaattact gcttcagaaa ataaggaggt agattttatt gtgttaatgg
     5041 ctggtcccgg cattccaggc gattctattt tattattaca aggcgattta attgcaaaag
     5101 catcgggtat atctgatgag agcataaaag cactgcacga attaagaaaa aatattatgc
     5161 aggtagcaaa agatgaaata gacaatgatg taatgcgaaa taatatgatt accctcaaca
     5221 aaaattttat tgccaataca ccaaaacata ttctcgatga acttgaaatg actgaagatg
     5281 atgtagaaga gggtttagat atatatattg gcgaatggat gcaatttttt cttgcctaca
     5341 atccacgacc aacattagaa cacactacca ttcctgtttt agcaataaat ggcacaaccg
     5401 acttgcaggt gccgtataaa gaaaatctcg aagcaattga aaaagcactc acaaaagccg
     5461 gtaataaaaa ctataaaata ctgccgttac aaaacctcaa tcacttgttt caacagtcac
     5521 tttccggcaa tcctgccgaa tatggcacca ttgaggaaac ctttaacgaa caggctatgt
     5581 ccatcatcgc caactggatt ttatccctga aataagcttg caggtaaggt tgtgcgcaat
     5641 ttaggtgtta aaatgtggca tcaggtggcg aagtcacaaa attgtacaaa cagatacctt
     5701 aattttgccc aactttttat aacattgcaa tgccggcaaa caggtgtcgg atgaccaaaa
     5761 cagcaggatc cgaccttgga gatagaaatc ataactgtag caggcctcac cattggcgag
     5821 ccggtaacca gtttaacaga tatcgtttta gcggtaatta gttttaccct gttcgctcga
     5881 attaaaaaca ggctcaacga gagctttttt aacaattcgt ggcgcatgtt tttccttttt
     5941 atgggttttt caacaggtac gggcgccatt gcacacgcac tcaatggctc agatgccatc
     6001 cgctggtata ataccctgtt tatggccatg accgttttta gcagtatttc tgtgtttttt
     6061 gcattaaaag caactatccg gtttactaaa atgttaccct ctatccgaac agtgctcacc
     6121 attgccaact acacattatt agctgtattc acagtgtata ccgtaataac caacaatttc
     6181 gaaattttta aaattcacgc agcagcaggt ttatttctca tatttattac acacctggta
     6241 gcaagctcct gcgcgcgcct gtgatcacct ccgcaccgat tggcc
//
LOCUS       DAORKW010000176         6253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_448_length_6253_cov_5.523879, whole genome shotgun sequence.
ACCESSION   DAORKW010000176 DAORKW010000000
VERSION     DAORKW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_448_length_6253_cov_5.523879"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="PLB46_08270"
     CDS             complement(<1..1541)
                     /locus_tag="PLB46_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRF76552.1"
                     /translation="MPEKLRKKDALDYHSSGRPGKIEVVPTKSTKTQRDLSLAYSPGV
                     AEPCLEIAEDISNVYKYTAKGNLVAVISNGTAVLGLGNIGPEASKPVMEGKGLLFKIY
                     ADIDSFDIELNCNDVDDFVKIVKAMEPTFGGINLEDIKAPECFEIEKRLKKELKIPIM
                     HDDQHGTAIIASAALLNATEIVGKNLADVRVVVSGAGASAISCAKLFVSLGVTRSNIM
                     MLDSQGVINKHREGLDEYKSQFINDTDLNTLDEAMVGADVFVGLSKGNIVTPEMLLTM
                     AKDPLVFALANPDPEIPYELAVRTREDVIVATGRSDHPNQVNNVLGFPYIFRGALDVQ
                     ATEINEDMKVAAVRALAALAKEPVPEFVNMAYSEKNIQFGRTYIIPKPVDPRLISRVA
                     PAVARAAIKSGVAKKNIGDWEEYATSLENRLGLEERLIKAITSKAKQNPKRVVFAEAD
                     NFKILKAAQIVKEEGIAKPILLGPVDKIRAIIEDNQLDLANVPIIDTRAKEQDEKRAQ
                     FGELF"
     gene            1820..2788
                     /locus_tag="PLB46_08275"
     CDS             1820..2788
                     /locus_tag="PLB46_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76553.1"
                     /translation="MTFNDIFLRPFYTRHFFVLAFLLSTSIFCYSQTNKYHPFLHEGG
                     KWSILKKEWETGLEDTVFYFYLYCTDGDTIVDGKTFFRVKYVNDDTCSNLYNALLRED
                     TITKKIYYYRFDVEFEGDTLLYDFSVVEGQSLSHCITFAHFDSSEVSSIDSIFLDGNY
                     RKKFNILFYVDGNSDSTFWIEGIGAVVGPAPFSGQPEDFGYGVYTPYIDKSQIICYKE
                     NLDVIYDEPYWIYNCAAGQISINNFVNDLLTIYPNPCPSGQQIIISNEGNIQYDCEII
                     SITGKLVNRFAINGNYEINTSTIPTGYYYITFKSYNNVYVNTFIIL"
     gene            3016..4644
                     /locus_tag="PLB46_08280"
     CDS             3016..4644
                     /locus_tag="PLB46_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76554.1"
                     /translation="MTRFLLFFAILMMGFSAFAQPSIVWQRCYGGTNNDYFTDAQNTL
                     DNGVITCITTGSVDGDLAGLDSAFGWVIKFDSLLEIEWQRLYGNPDLEIIPRYCKQLV
                     DSNYIFYGFGGIGTNGFQGQLDFMLMKTDKFGNLLWHKSYGGPGLEAIDGFIATTDGG
                     FLFTGQSAQAGGDIPFHYGDVFFDDANIIKTDNEGNIEWIKNLGGSGVDGCIGNPVEI
                     SPGKYQVHIYSGSDDYDLIDCGITAFQKRWVINLNNEGVIEQQVFYEAEENLLRSDGQ
                     TGVLSDNRTIIIGSGNAMSPLFPAPEGHQGEEGAVGIFNNDLLLTNVKMFGGSGAERF
                     YKYCNDIYNDFYFLGYSLSQDYDLPGNYNLGEANDYWLVKTDGNFNKIWSINFGGSNS
                     AGDLATTSWHGNLVFHNNNLLYFGSSVTPEELPDFDIACGNLLPSPSPVVKLDAWMVA
                     FDLQTAIEDYFPHKNNIQLYPNPADKFTSVRIAGNESFEYQVFSSTGLLISEGNVNSI
                     NTQINTADLQNGIYYIRFIQSILPSKTSPLIIIH"
     gene            complement(4738..5298)
                     /locus_tag="PLB46_08285"
     CDS             complement(4738..5298)
                     /locus_tag="PLB46_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HRF76555.1"
                     /translation="MKKWIHTGLLLIVISFFCADVHAQVNAQKPFPSIKIGNQEWMTV
                     NWDYKTPKSFFYNNDSTLDSKYGRLYYFSNALAAAPPGWHLPTLDEWYELIDTLGGLS
                     KAGLKMFDPTYGLNLVAGGYKSANINEGEILFGFLDTYAFYWTATPDGEQTAYAVHIT
                     KATGEFDITSFRRANGFSVRYVKNKE"
     gene            complement(5334..5852)
                     /locus_tag="PLB46_08290"
     CDS             complement(5334..5852)
                     /locus_tag="PLB46_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76556.1"
                     /translation="MKTSLSPFWVGITVVMVTYSFYSCNEMGIVEIQDTNVVTQTQNE
                     TEEDIFVGRYAMHYKYTMPNLDGFDPEFIEVDQIDVSFRIHFCTDINEYQITSPVEFQ
                     SEGICANGTIALSINHLFSHSDPGISEKRVIGSTYWENDSLHFSVLYKVTNWPPAPSY
                     EYTISGAGIKIE"
BASE COUNT         1863 a         1147 c         1182 g         2061 t
ORIGIN      
        1 aaaaacaatt cgccaaactg tgcacgtttt tcgtcctgtt cttttgctct ggtgtcaata
       61 atcggcacat tggcaagatc gagctgatta tcttcaataa tcgctctgat tttatccacg
      121 ggacctaaca aaataggttt ggctatacct tcttccttaa caatttgagc agcctttaaa
      181 attttaaaat tatctgcttc ggcaaaaacc acccttttcg gattttgttt ggctttgctg
      241 gtaatggctt ttatcagtcg ctcttctagt cccaggcggt tttccaacga tgttgcatat
      301 tcttcccaat ctccgatatt ctttttggct acgccggatt taatagctgc tcgcgcaaca
      361 gcaggtgcaa cgcgtgaaat taatcgtggg tcaaccggtt tagggataat ataggttctg
      421 ccaaactgaa tatttttttc gctgtaggcc atatttacaa actccggcac aggttctttc
      481 gctagtgcag caagggctcg cactgcggct actttcatat cttcatttat ttcggtagcc
      541 tgcacatcga gtgctccgcg gaaaatatat ggaaaaccta acacattgtt tacctggtta
      601 ggatggtcgc tgcggccagt ggctacaatt acatcttcac gggttctaac cgctaattcg
      661 taaggaattt cagggtcggg gttagccaaa gcaaatacca gcgggtcttt agccatggtt
      721 aacaacattt caggtgttac gatattacct ttcgataaac caacaaatac atcggcacca
      781 accattgcct cgtcgagggt gttgaggtcg gtatcgttga tgaactgact tttatattca
      841 tctaaacctt cacggtgttt gttgattaca ccctgactgt ccagcatcat aatattactt
      901 cttgttacgc cgagcgatac gaatagtttg gcgcaggaaa ttgccgaggc tccggcacct
      961 gaaactacta cacgcacatc agccagattt ttaccgacta tttcggtggc attcagcaaa
     1021 gcagcgctgg caataatggc agttccgtgc tggtcgtcgt gcatgatagg gattttgagt
     1081 tcctttttca gccttttttc aatttcaaaa cattccggcg ctttaatgtc ttccaggttt
     1141 attcctccga aagtaggctc cattgcttta acgattttta caaaatcgtc aacatcgttg
     1201 caattgagtt ctatgtcaaa tgagtcgata tcggcataaa ttttaaacag caaccctttt
     1261 ccttccatca cgggtttcga ggcttctgga ccaatattgc ccaaacctaa cactgccgtt
     1321 ccattgctga taaccgctac cagattgcct ttggcggtgt atttatacac attggaaatg
     1381 tcttcggcaa tttcgagaca aggttcagct acccccggcg aatacgccag cgacaagtcg
     1441 cgttgtgttt ttgtcgattt ggttggaact acttcaatct ttcccggtct gccggatgaa
     1501 tgataatcaa gtgcgtcttt ttttctcagt ttttcgggca taagcctccc tttttaccgc
     1561 tttcgggttg gtgatgtaat acattacgct tcttaggatt tcaaattcaa tttaactcct
     1621 aataaggacg gcaaaaatag tgactaagaa cgggattaca gatgatatta atcggaaaat
     1681 gacttattcc ttatttttca cataccgcgc gctaatccgt ttacctgcag ggagggtagg
     1741 gaaatcaatt acgctatagt taaaaacgcg cattaagttg gttgttatta attaaatttg
     1801 gggttatcaa ttccacaaaa tgacctttaa cgatattttt ttacgacctt tttatacaag
     1861 gcatttcttt gtactggcat ttttactttc aacaagcatt ttttgttatt cccaaacaaa
     1921 taaatatcat ccatttttgc atgaaggtgg taaatggtca atacttaaaa aagaatggga
     1981 aacaggactg gaagatacag tattttattt ttacttatat tgcacagatg gagatactat
     2041 tgttgatgga aaaacctttt ttagagtaaa atatgtaaat gatgatactt gctctaatct
     2101 ttataatgca cttctaagag aggatactat cactaaaaaa atttattatt acagatttga
     2161 tgtagaattc gaaggagata cccttttgta tgatttttct gtagtagaag ggcaaagttt
     2221 aagtcattgt ataacatttg ctcactttga ttcttctgaa gtatcttcaa ttgattcgat
     2281 ttttttagat ggtaattata gaaaaaaatt taatatattg ttttatgttg acggaaatag
     2341 tgattctaca ttctggatag aagggattgg agctgtagtt gggccagcgc cgttttccgg
     2401 tcagccagaa gattttggat acggcgtgta tacgccttat atagataaat cacaaattat
     2461 ttgttataaa gaaaatttgg atgtaatata tgatgaacca tattggatat ataattgcgc
     2521 tgccgggcaa atttcaataa ataattttgt aaatgatttg ctaacaatat atcctaatcc
     2581 ttgcccatca ggtcagcaaa ttattatatc aaatgaagga aacatccaat acgattgtga
     2641 gattatttcg attaccggaa aattagttaa ccggtttgca ataaacggca attacgaaat
     2701 taataccagc actattccaa caggttatta ttatattact tttaaaagct acaataatgt
     2761 ttatgtaaat acatttatta ttttataatt aaaataacct attccttatt tttcacatac
     2821 cgcacactaa atccattcgc tcttctaaag gaagtaatat caaattcgcc ggcgggtttt
     2881 gtgctgtgga ggttagccaa tatgatattt caagtaaact aaggttaata aattgagttg
     2941 gtatttgttt ttttgaaaat aaagattaaa tttggatagc aattatatcc tcattaccct
     3001 taaatatctt accttatgac gcgttttcta ttgttttttg ctattctgat gatggggttt
     3061 agtgcgtttg cacagccgag tattgtatgg caacggtgtt atggtggaac aaacaatgac
     3121 tattttactg atgctcaaaa cacattagac aatggagtaa ttacctgtat aacgactggt
     3181 tctgttgatg gagatttagc aggtctggat agtgcatttg gatgggtaat taaatttgat
     3241 tcattattgg aaattgagtg gcaaagacta tacggaaatc ctgatttgga aattatacca
     3301 aggtattgca agcagttggt ggattctaat tacatttttt atggttttgg tggaatagga
     3361 acaaatggtt ttcaaggcca attggatttt atgttaatga aaaccgataa gtttgggaac
     3421 ttattgtggc ataaaagtta tggcggacct gggttggagg ccatagatgg atttatcgca
     3481 actacagatg gtggtttttt gtttacagga caaagcgccc aagcaggtgg tgatatacct
     3541 tttcactatg gtgatgtatt ttttgatgat gcgaacataa ttaaaacaga caacgaaggt
     3601 aatattgaat ggattaaaaa tttgggtgga tctggtgttg acggatgtat tggaaaccct
     3661 gttgaaatta gtccagggaa ataccaggta catatatatt caggttctga tgattatgat
     3721 ttaattgatt gtggtataac agcctttcaa aaacgatggg taataaattt gaataatgaa
     3781 ggggtaatcg agcagcaagt gttttatgaa gccgaagaga atttattgcg gtctgatggg
     3841 caaacaggag tattgagcga caacagaaca attattattg gttcaggcaa tgctatgtca
     3901 ccactctttc cggcaccaga aggtcatcag ggtgaggagg gtgctgttgg aatatttaat
     3961 aatgatttgt tattaacaaa tgtaaaaatg tttggaggca gtggagctga acgcttttat
     4021 aaatattgta atgatattta taatgatttt tattttttag gatatagctt atctcaggat
     4081 tatgatttac ctgggaatta taatctaggt gaagcaaacg attattggtt agttaaaaca
     4141 gatggtaatt ttaataaaat ttggagcata aattttggtg gaagcaactc ggctggagat
     4201 ttagcaacta caagttggca tggaaacctt gtatttcata ataacaattt attgtatttt
     4261 ggttcatctg taacacctga agagttgcct gattttgata tcgcttgtgg taatttatta
     4321 ccatccccta gtcctgtagt taaattagat gcatggatgg tagcttttga tttgcaaaca
     4381 gcaatagaag attactttcc tcataaaaat aatattcaac tttatcctaa tcctgctgat
     4441 aaattcacat cagttagaat agcgggtaac gagtcatttg aatatcaagt cttttcatca
     4501 accgggttat tgataagtga aggcaatgtc aactcaatta atacccaaat taatactgcc
     4561 gacttacaaa atggaatata ttatattcga tttatacaat caattttgcc ttctaaaact
     4621 tccccattaa taattattca ctaagtagtt ttcgattaag tgattccaaa agtaatcttt
     4681 tttacttttg tgaaaacctc attttttgta tcatctatta gaaataaata gagtactcta
     4741 ttccttattt ttcacatacc gcacactaaa tccattcgct cttctaaagg aagtaatatc
     4801 aaattcgccg gttgctttgg ttatgtgaac agcgtaggca gtttgctcac catcgggtgt
     4861 tgctgtccag taaaatgcat aagtatctaa aaagccgaac aggatttcac cttcattaat
     4921 gtttgcactt ttgtatcctc cggctaccaa atttaatccg taagttgggt caaacatttt
     4981 gagaccggct ttgctcaatc cgccaagggt gtcaattaat tcgtaccatt catccaaagt
     5041 tggtaaatgc catcccggag gggcagcagc caatgcattg ctgaaataat acaaacgtcc
     5101 atattttgaa tccagtgtag aatcgttgtt gtagaaaaag cttttgggtg ttttgtaatc
     5161 ccaattaacg gtcatccact cctgattgcc aatttttatt gagggaaaag gtttttgagc
     5221 attaacctga gcatgcacat ctgcacaaaa aaagctgatt acaatcagta acaatcccgt
     5281 gtgtatccat tttttcataa cttcctgtct gttgcaaata tcgggctaaa tttttattca
     5341 atttttatac cggctcccga tatcgtgtat tcgtaacttg gcgcaggagg ccaattggta
     5401 actttatata aaacactaaa atgtagactg tcattttccc aataagtaga cccaattacg
     5461 cgcttttcgg aaatgccggg atctgaatga ctaaataaat gattaatgga gagggcaatt
     5521 gtgccgtttg cgcaaatacc ttctgattga aactccacag gcgaggttat ttgatattca
     5581 ttaatatctg tgcaaaaatg aatacgaaaa ctaacatcta tttgatcaac ttcaataaac
     5641 tcagggtcaa agccatctag gtttggcata gtgtatttat aatgcatggc atatcgccca
     5701 acaaatatgt cctcctctgt ttcattttga gtttgagtta caacatttgt gtcttgaatt
     5761 tctacaatac ccatttcatt acatgagtaa aaagaataag taaccataac cactgtaatc
     5821 cctacccaaa atggcgataa agacgttttc ataagagtaa ttttaatact actaagatat
     5881 taaaattttg cagaagtaat ttataactat cccaaataat ccaatccgca cctgtctgcc
     5941 cccaagcagg atcccccaaa aatccgtccc catccgttcc acccgttcaa tccgtgcctg
     6001 tctgccgcca ggcaggttct ttttcatcca atccatccag aaaatcagtt catttttcct
     6061 aaccaaacgc aaaaccttta tgtaagggcg gattcaattc gcccctacat aaaggtttta
     6121 ctcacattat cgaaaaaaat tgaaatttat ctacatcaaa aaaattcagc acctgtctgc
     6181 cgccaggtag gttccctaaa aaatccgtcc ccatccgttc cacccgttca atccgtgcct
     6241 gtctgccgcc agg
//
LOCUS       DAORKW010000177         6247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_450_length_6247_cov_5.849968, whole genome shotgun sequence.
ACCESSION   DAORKW010000177 DAORKW010000000
VERSION     DAORKW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_450_length_6247_cov_5.849968"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..1661
                     /locus_tag="PLB46_08295"
     CDS             270..1661
                     /locus_tag="PLB46_08295"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76557.1"
                     /translation="MKQIVFALLIFINCQLATAQYTKLVDMNGVETGAYPYHAEFYYD
                     GNFLYGTTSAGGLTGHGIIFRLKTDGTEFSKIVDFDDAVTGSSPKSGLISDGTYLYGT
                     TSSGGPLGGGVIFKVKPDGSDFILIHEFIGGDDGGNLKGKLFFDGSYLYGLSPQGGDN
                     SAGMIYKLKPDGSEYAVIFSFDGEETGVTPNGALVSDGTYLYGMTVSGGTDFLGAAFK
                     VKKDGTDFVKIMDMLDDPNGAFGYGSLIYDGTYLYGMTNNGGEENRGTIFKMLTDGSG
                     YQKLLDFNSDNGAQPLGSLILDGTTLYGMAELGGPDNFGFVFQIQTDGSDYQQLVDFN
                     GAENGSLPFGTLLMVNKALFGMTNSGGTSDAGVAFRYGEEEIPTAITSINSTSFEILP
                     NPNNGQFNLNIGNQFQNEKFTLVIYNTIGQKVYTANQVSSGKITLSQPKPGIYIAQIN
                     SALGNYTTQFMIE"
     gene            complement(1759..2697)
                     /locus_tag="PLB46_08300"
     CDS             complement(1759..2697)
                     /locus_tag="PLB46_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76558.1"
                     /translation="MKENNSRRQFLRNTALAGFSLGLINTSTKANSRQPLTVLGACNP
                     TTLDYYGQGPFYTADAPDIIDNLLADLAEPGTRLLISGRVQTLDCTATIANTIIDIWH
                     ANAAGAYDNTGFNLRGKTLSNAEGFYIFETVLPGKYLNGASYRPSHIHFKITPPGFAT
                     LTTQLYFEGDTDIPGDAAASITTGTFDATNRIIPLIENGAGKLEGTWDIAINGDGEVG
                     IADLHTDKGLIYSASPNPFTNQIAVHYGVFQPTQVKLKVINLQGNVVATLVDAKMAPG
                     KFQTTWAPTNSIPDGHYFLTLQINDLQVHYVKIMKV"
     gene            3090..4706
                     /locus_tag="PLB46_08305"
     CDS             3090..4706
                     /locus_tag="PLB46_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76559.1"
                     /translation="MKKLLLILTCVTHQLYAQTELTLVKDINPGEPNSAPGLFAHVND
                     QLFFIANNGTNGIELWKTDGTEANTIMIKDIGSGSNSYCESTFGCGSEFIVMNNIIYF
                     RATDASHGAELWRSDGTEAGTYLVKDINPGPGDCSNSVFMSGQYFAQMNDVIYFAADG
                     GGNNIELWRSDGTELGTYMVKDFAAGASSIPQYITAIDDNIYFQCKNTAGESEVWKSD
                     GTEAGSVLLKQMWMRAFEYTNMFVQFNDHIYFPGAATDPYDLELWRTDGTAAGTELFY
                     ELNPEEGDGSDPKQFHIVNDKLLFVASPESERQLFTSDGTVAGTTVLEDENGNPFSPA
                     DYGFLQSDSKLYFQGDNATHEGLWITDGNSSGTKFLLQIESGGFDENAAAKITGNNIV
                     FSAYDDDNGCTTLFQSNGSEDGTFQATTCDDISYPTALTEYNGTIIFNGTNDAVGAEL
                     FQFITEFEVAINNIATPATFNVFPNPAIDFINLNFSNQDASDATIIIKDITGKIAGQY
                     STSNNLEINYLSPGIYFIQAGNLITQFVKL"
     gene            complement(4709..5515)
                     /locus_tag="PLB46_08310"
     CDS             complement(4709..5515)
                     /locus_tag="PLB46_08310"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRF76560.1"
                     /translation="MLKRTRIFELDLINDSNFDATIASMVNFQQEYQPDGDKLPLLFT
                     PNVDDVVKLNDKKYSALAAILKKCFYIIPDGQPIIWVSKMLGKDKVLQRRLPGSELFP
                     LLWKELIRHNKRIMVIAPNEQVGELLKKEYANMVYYVPPFFDENDKAAMDAVIEGCQP
                     LFDDFQPEYVFLGIRFPKQNHIALGLIEHVKQHNAAQGNPQRIMPLFLLLGASYEFYL
                     NIKKRAPKFWQKIGMEWFYRFTQEPGRLFKRYFVDDMQFIPIVFKEFRKK"
     gene            complement(5518..>6247)
                     /locus_tag="PLB46_08315"
     CDS             complement(5518..>6247)
                     /locus_tag="PLB46_08315"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRF76561.1"
                     /translation="SYPTVSWHRPQETMDTNVNGTINVFEAIKSVRKTNKNYDPVVVV
                     ACSSAEYGASLTPENTPVKETAALLPLHPYGVSKVGQDLLSFQYYQNDKIRCIRARIF
                     NTTGPRKTNDVVADFTHRAVDIERKKIDVLKVGNLKTKRAITDVRDLVNALMLLSDKG
                     TWGDVYNISGNKVYEINEIVPIIENYMGIHLATEVDPKLLRPTDEPIIYGDSSKLKND
                     TGWEQQYPLEQTIKDMIDYIRNKS"
BASE COUNT         1952 a         1096 c         1166 g         2033 t
ORIGIN      
        1 tcattcccga aatttagatg tatatattcg aaaactgcgt gagtattttg ggactggtaa
       61 tggggtagaa ataattacgt tgaagggaaa ggggtatcag tttgtggtga atgggtgaag
      121 ctgttggatg gttgtgtttg ccgggaagac gggaagatgg gaagttgcgg ggatttttga
      181 gtactcacct tattgtggta tggtatgggg atattctttt gatgaatttt gtttcaggaa
      241 tttaatcttt tttattaaat gattattgta tgaaacaaat tgtctttgcc cttttaattt
      301 ttattaactg tcaactagct actgcacaat acactaaatt agttgatatg aatggcgttg
      361 aaaccggtgc ctatccatat cacgcagaat tttattatga tggcaatttt ttatacggca
      421 caacatctgc aggtggatta acaggtcatg gtataatttt ccgtttaaaa acagacggaa
      481 ccgaattttc caaaattgtg gattttgacg acgctgtaac aggtagtagt cctaaaagcg
      541 gacttatttc tgatggcact tatttgtatg gcactacaag tagtggtgga ccacttggtg
      601 gaggtgtaat ttttaaagtt aaacccgatg gttcagattt tattttaatt catgaattta
      661 tcgggggtga tgatggtgga aatttaaaag gtaaactatt ttttgatggc agctatttgt
      721 atggcctatc accacaagga ggtgacaact cagcaggaat gatatataaa ttaaaaccgg
      781 atggcagtga atatgcagtg atttttagtt ttgatggaga agaaaccggt gttacaccaa
      841 atggtgctct tgtttctgat ggcacttatt tgtatggaat gacagtgagt ggtggaaccg
      901 attttttagg cgccgcattt aaagttaaaa aagacggaac agattttgta aaaataatgg
      961 atatgctcga tgacccgaat ggcgcatttg gatatggaag tttaatttat gatggtactt
     1021 atctctatgg catgaccaat aacggaggcg aagaaaatcg tggtactatt ttcaaaatgt
     1081 taacggatgg ttcaggttat caaaaactac ttgattttaa tagtgataac ggtgcacaac
     1141 cacttggctc attaatttta gatggaacta ctttgtatgg catggccgaa ctgggaggac
     1201 cggataattt tggtttcgtt tttcaaattc aaactgatgg cagcgactat cagcaactag
     1261 tagattttaa tggtgctgaa aatggcagtt taccttttgg cactttgtta atggttaaca
     1321 aagctttgtt tggcatgacc aacagcggtg gtaccagcga tgcaggtgtg gcttttcgtt
     1381 atggtgaaga agaaattcct actgctatca catcaataaa tagtacttca tttgaaattt
     1441 tgccaaaccc aaataacggt caatttaatt tgaatatcgg taaccaattt cagaatgaaa
     1501 aattcacact tgttatttat aatacgattg gacaaaaagt atatacggcc aatcaagttt
     1561 cttcaggtaa aataacgctc agtcagccca aacccgggat atacatcgca caaattaatt
     1621 cagcacttgg aaattatacc actcaattta tgatagaata aattacttat tgtatttttt
     1681 acaataagtc aacaaatttg aaaaaaaact tatctgttat ttggttcatt tcctaaaaca
     1741 agcaaacggt aaactggatc acactttcat aattttcaca taatgcactt gcaaatcgtt
     1801 aatttgtagt gtgagaaaat aatgtccatc cggaatacta tttgtcggtg cccatgtagt
     1861 ttgaaattta ccgggtgcca tttttgcatc tactaaagtt gcaactacat ttccctgcaa
     1921 attaataact tttaatttca cctgtgtagg ttggaacacc ccataatgaa ctgcaatttg
     1981 attggtaaaa ggatttggag aagcggaata aattaatcct ttatcggtat gcaaatcagc
     2041 tatacctact tcaccatcac catttatagc aatgtcccag gtgccttcta attttcctgc
     2101 tccattttct attaatggaa ttattctgtt agttgcatca aatgttccgg tggtaattga
     2161 agctgctgca tctccgggaa tatctgtatc accttcaaaa tataattgtg tagtaagtgt
     2221 ggcaaatccc ggtggtgtaa ttttaaaatg gatatgactt ggccgataag aagcgccgtt
     2281 taaatattta ccgggcaata ctgtttcaaa tatataaaat ccttccgcat tagataaggt
     2341 ttttccacgc aaattaaatc cggtattatc ataagcacct gctgcattag catgccagat
     2401 atcaattata gtattggcaa tagtagcggt gcaatctaat gtttgcactc tcccacttat
     2461 taataatcgt gttccgggtt ctgctaaatc tgctaataaa ttgtcaataa tatccggcgc
     2521 atcagccgtg taaaacggtc cctgaccata ataatcaagg gtagtaggat tacacgcacc
     2581 taacaccgtt aaaggctgac gcgaatttgc tttagtgctt gtattgatca aacctaaaga
     2641 gaaacctgcc aaagctgtat tgcgcaggaa ttgacgacgg gagttatttt ctttcataag
     2701 tatgctgtat gtaaatatag ttactaattt ggaaagggtt caaataaatt atgttaaaat
     2761 gtcaaaagat taaatcccct tgcattccgc ttcctcacaa cttttcattt tcatcaacag
     2821 catataagca ccttttacca caaatatttt atctgcagta tctaattcaa cttcaaaaac
     2881 aatttgttca tacgaattgt ggccaaatgg tatccatctc aaaacaaaga aacaacaaaa
     2941 tttccatata tttactgata atcaatcact tacaaaaagt ctttatataa ttacatttag
     3001 ttgaaccacc atcctgctac aataagcatt tttcctactt tgttattctc cgataaaatc
     3061 cgtattttgt ttgttctaaa actaccgcta tgaaaaaatt attactcatt ttaacttgtg
     3121 ttacacacca attgtatgct caaacggaat taacactagt aaaagatatt aatcccggtg
     3181 aacctaatag tgctccagga ttatttgcac atgttaatga tcaattattt tttattgcca
     3241 ataatggcac caacggaatc gaattatgga aaacagatgg cactgaagct aataccataa
     3301 tgataaaaga tattggctcc ggcagcaata gttattgtga atcaacgttt ggttgcggaa
     3361 gtgaatttat tgtgatgaac aatattattt atttcagagc aactgatgca tcacatggtg
     3421 ctgaactgtg gagaagtgat ggaacggaag caggaactta tttggtaaaa gatattaatc
     3481 ccggtcctgg cgattgctcc aattcggttt ttatgtcggg acaatatttt gctcaaatga
     3541 atgatgtaat atattttgct gctgatggtg gtggcaataa tattgaatta tggagaagtg
     3601 atggaaccga gcttggcact tatatggtaa aagattttgc cgcaggtgca agcagtattc
     3661 cgcaatatat tacagctatt gacgacaata tttattttca atgtaaaaat actgcgggcg
     3721 aatctgaagt ttggaaaagt gatggcactg aagcaggttc cgtattatta aaacaaatgt
     3781 ggatgcgtgc atttgaatat accaatatgt ttgttcaatt taacgatcat atttattttc
     3841 caggtgccgc aaccgaccct tacgatttag aattatggcg caccgatggc actgctgcag
     3901 gcactgaatt attttatgaa ttaaatcccg aagaaggtga cggcagcgac ccaaaacaat
     3961 ttcatattgt aaatgataaa ttactttttg ttgctagccc ggaatcggaa cgacaattat
     4021 ttacttcaga tggaactgta gcaggaacaa cagttttgga ggatgaaaat ggtaatcctt
     4081 tttcaccggc tgattatggc tttttacaat ctgatagtaa attatatttc cagggcgaca
     4141 atgccacaca tgaaggactt tggattacag acggtaacag cagcggaact aaatttttat
     4201 tacaaattga atccggcggt tttgatgaaa atgctgccgc aaaaattaca ggaaataata
     4261 ttgtttttag cgcttacgac gatgataatg gttgcaccac tttgtttcaa agcaatggta
     4321 gtgaagatgg cacttttcag gcaactacct gcgatgacat cagttaccct actgcattaa
     4381 cagaatataa tggcactatt atttttaatg gaaccaatga tgctgttggt gcggaattat
     4441 ttcaattcat tactgaattt gaagttgcca ttaacaatat tgccacacca gcaacattta
     4501 atgtttttcc aaatcctgct attgatttta tcaatttaaa tttttcaaat caagatgctt
     4561 cagacgcaac cattatcata aaagacataa ccggaaaaat tgctggtcaa tattctacaa
     4621 gtaataatct ggaaataaat tatttatcgc ccggcattta ttttatacag gctggtaatt
     4681 tgataacgca atttgtaaaa ttataagatt actttttccg aaattcttta aatactatcg
     4741 gaataaattg catatcatcc acaaaataac gtttgaataa acgtcccggc tcttgtgtaa
     4801 atcggtagaa ccattccata cctatttttt gccagaattt cggagcgcgt ttttttatgt
     4861 tgagataaaa ttcataagaa gctcctaaca ataaaaataa tggcataatg cgctgtggat
     4921 tgccctgtgc ggcattatgt tgttttacat gttcaatcaa ccccaacgca atatgatttt
     4981 gttttggaaa acgaatgccc aaaaatacat actccggctg aaaatcatca aataatggtt
     5041 gacatccttc aattaccgca tccatggctg ctttatcgtt ttcatcaaaa aacggtggta
     5101 cataatacac catgttggca tattcctttt ttaataattc accaacctgt tcattgggag
     5161 caataaccat gatgcgttta ttatggcgaa ttaattcttt ccataacaac ggaaataatt
     5221 cagaacccgg caacctgcgt tgtaacacct tatcttttcc caacattttg ctcacccaaa
     5281 taatgggctg accgtcagga atgatataaa aacatttttt caaaatcgcc gcaagcgcac
     5341 tgtatttttt gtcgttgagt tttacaacat catccacatt tggtgtaaat aataaaggta
     5401 gtttatcacc atcgggctga tactcctgtt ggaaattaac cattgatgca atagtcgcat
     5461 caaaattaga atcattaatt aaatcgagct caaaaatgcg ggttcgcttc agcatgttca
     5521 cgatttattt ctgatataat ctatcatgtc tttaatggtt tgctccaacg gatattgctg
     5581 ttcccaaccg gtatcgtttt tgagtttcga cgaatcacca taaataatag gctcatcggt
     5641 agggcgcaat aatttaggat ctacttcagt agctaaatga atacccatat aattttctat
     5701 gataggcaca atttcattta tttcgtaaac cttgttgcct gaaatattat atacatctcc
     5761 ccatgttcct ttatcactca gtaacattaa ggcattaacc aaatcgcgca cgtcggtaat
     5821 ggcgcgtttt gtttttaaat tacccacttt taatacatca atttttttgc gttcgatatc
     5881 tacagcacga tgtgtaaagt cagcaacaac atcattggtt tttcttggac ctgttgtatt
     5941 aaaaatgcgc gcacgaatgc agcgtatttt atcgttttga taatattgaa aggatagtaa
     6001 atcctgacct actttactca ccccatatgg atgtaatggt aacaaagctg cagtttcttt
     6061 tacaggtgta ttttcaggcg ttaacgaagc accgtattct gcactagaac aggcaactac
     6121 cactacaggg tcgtaatttt tattagtctt gcggacagat tttattgctt caaacacatt
     6181 gatagtacca tttacattgg tatccattgt ttcctgcggc cgatgccaac tcacggtcgg
     6241 ataactc
//
LOCUS       DAORKW010000178         6172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_464_length_6172_cov_4.807912, whole genome shotgun sequence.
ACCESSION   DAORKW010000178 DAORKW010000000
VERSION     DAORKW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_464_length_6172_cov_4.807912"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /gene="aspS"
                     /locus_tag="PLB46_08320"
     CDS             <1..1199
                     /gene="aspS"
                     /locus_tag="PLB46_08320"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRF76562.1"
                     /translation="HIKVDNVDHIQILKQLLMVSGMDRYYQIAKCFRDEDLRGDRQPE
                     FTQIDCEMSFVRQEDVWNMFEGMTKYVFKQTKNIDLPDFLRLPYKEAMQYYGSDKPDL
                     RFDCKIIDLNDVVGGTEFSVFNNIITSNGLIAGINAKGCSEYSRKQIDELTEFVKAPH
                     RGVGGLIYIKFNQDGSIKSSIDKFYSVEQLTAIGNKLNAQKGDLVLIIADKVNKTRKV
                     LGELRLEMAKRENWIDKNAWSVFWVVDMPLFEPDENTGEPIFAHHPFCSPHPDDFQYM
                     DTEPLRVRAQSYDLVMNGNEILSGSIRVHDKTLQEKIFTILGFSEAEKEKRFGFMVNA
                     FKYGAPPHGGCAFGFDRWVMLMAGGDTIRDVIPFPKNNNGRDMMMDAPGDVDNEQLKI
                     VGVKLL"
     gene            complement(1386..1790)
                     /locus_tag="PLB46_08325"
     CDS             complement(1386..1790)
                     /locus_tag="PLB46_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HRF76563.1"
                     /translation="MVELDENELAKLVVNIAFEIHVKLGPGLYESVYEEILFKELVKC
                     GLQVERQKAIYLYWDNERVNKYAFRADLIINNKLLIEVKSIEALADVHFKQVNTYLKV
                     TGIKLGLIINFNVALIKHGIKRIVNGLELKST"
     gene            complement(1973..3451)
                     /gene="hutH"
                     /locus_tag="PLB46_08330"
     CDS             complement(1973..3451)
                     /gene="hutH"
                     /locus_tag="PLB46_08330"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HRF76564.1"
                     /translation="MDTYHIGSQPLSITLIDTIIKSKAKLALDDTAREKIQLCRTYLD
                     KKIADSGKAVYGVNTGFGSLCKVKISDDDLEQLQYNLIRSHACGVGPVIPDEICKILL
                     LLKIQSLSYGNSGVSVETVWRLIDFYNLEILPVIYEQGSLGASGDLCPLSHMSLALIG
                     EGSVKYNGEIRPTAEVLKDFKLSALKLQSKEGLALLNGTQFMSSYAVYNLIKSQNIIH
                     KSNKIAALSLEAYDCKAEPFFPQIHRVRNQSGQIITAKEIMDLLEGSETFFAEKQQVQ
                     DPYSFRCIPQVHGACRDAIDYCKSIVEKEINGVTDNPNIFVEDDLIVSGGNFHGEPLA
                     LTLDFLAMALSELGNIAERRVYRLLSGARNLPEFLTPNPGLNSGLMIPQYTAASIVSQ
                     NKQLCTPASVDSISSCNEQEDHVSMGANAATKCLRVVENIEKILAIELLTAAQAFEFR
                     RPLKSSKAIEDFYTAFRAVVSFNESDRFLHLDIDKSLMFINA"
     gene            3494..4402
                     /locus_tag="PLB46_08335"
     CDS             3494..4402
                     /locus_tag="PLB46_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF76565.1"
                     /translation="MGDIMTFSQVNKRFSDVQALQDVSFTIPENTIFGLLGPNGAGKT
                     TLIRIITKIFAADSGHIVFDGSDIQTQQYANIGYMPEEKGMYKKMKVGEHLVYLGRLK
                     GLKKSAAQERSKYWLEKLGAGDWWNKKLEDLSKGMQQKTQFIATVLHNPKLLILDEPF
                     SGLDPVNAEMIKNEIYQLHQQGTTIIFSTHRMEQVEEICKTIALINKGKLILNGEVAT
                     IKQQFKQHKFLISFESLPEIEDNDLFTVDQINGQSVTVQLKSKNDQNQVLQYFISRNA
                     IINAYYEILPSLNDIFISIVNKANHE"
     gene            4395..5690
                     /locus_tag="PLB46_08340"
     CDS             4395..5690
                     /locus_tag="PLB46_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF76566.1"
                     /translation="MNKTLLIIQREYITRVRKKSFIIFTLLGPVFFLLISLLPILIAN
                     ANKNNLHILVKDDSGLIPALPDTAGLYFTYKYQSIPVNTLKTDYSTIGEGYDAFLYIP
                     AMQPDAPYGIEIYSNEQVSLTTKLYIESIIADKLETINLQQHDLDKNILLKLRPKVDI
                     DDKVTSGESQKEGNAAIASAFGFGMGFIIYIVLLIYGTMVMRGVMEEKSSRIIEVIIS
                     SVKPFQLMMGKIVGIGLVGLTQFVAWGFLITILNIIIGILFAGQLSDLQQVSASSGEV
                     EMNAITEAMQSMQNIPIGYYLSMFIIYFLGGYFIYASLFAAIGSLAGDEEGDVQTFAF
                     PVTMLILVSIFIMMAVVQQPHTPLAFWASIIPLSSPIVMPALIPFGVPTWQIVLSIVL
                     LFGGFIFTTFLAGRIYRTAILMYGKKIKVKEVFKWMFYK"
     gene            complement(5693..>6172)
                     /gene="rodA"
                     /locus_tag="PLB46_08345"
     CDS             complement(5693..>6172)
                     /gene="rodA"
                     /locus_tag="PLB46_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRF76567.1"
                     /translation="QRIETLLGMSDNADAEYNVTQSKMTIGSGGVAGKGYLQGTLTQS
                     EQVPEQSTDFIFCTIGEEFGFLGTFVFLVVYLLFLLRIIFIAERQRSPFSRVYAYCVA
                     SFFFLQIMINVGMTIGLIPVIGIPLPFISYGGSSVLAFSILVFIMLRLDADRLLILR"
BASE COUNT         2042 a         1112 c         1084 g         1934 t
ORIGIN      
        1 tccacatcaa agttgataat gttgatcata tacaaatatt aaaacaatta ttaatggtga
       61 gcggcatgga ccgttattat caaattgcaa aatgttttcg cgatgaagat ttacgcggcg
      121 accgtcaacc ggaatttaca caaatcgact gcgaaatgag ttttgttcgt caggaagatg
      181 tgtggaatat gtttgaaggt atgacgaaat atgtattcaa acaaaccaaa aatatcgact
      241 tacctgattt ccttcgatta ccttataaag aagctatgca atattatggc agcgacaaac
      301 cggatttgcg tttcgactgt aagattattg atttgaatga tgttgttgga ggcactgaat
      361 ttagtgtgtt taataatata ataaccagca atggtttaat tgcaggtatt aatgcaaaag
      421 gttgcagcga atattcccgc aaacaaattg atgaattaac agaatttgtt aaagcaccac
      481 atcgtggagt tgggggctta atttatatta aatttaatca ggatggcagc ataaaatctt
      541 ctattgataa attttattca gttgaacaac ttacagcaat tggcaataaa ttgaatgcac
      601 aaaaaggtga tttagtttta atcatcgccg ataaagtaaa taaaacaaga aaagttttag
      661 gcgagttgcg tttagaaatg gctaaacgtg aaaactggat tgataaaaat gcatggagtg
      721 tattttgggt tgtagatatg cctttatttg aaccggatga aaataccggc gaaccaattt
      781 ttgcacatca tccattctgt tctcctcatc ctgacgattt tcaatatatg gacacagaac
      841 cattacgtgt gcgcgcacag agttacgatt tagtaatgaa tggcaacgaa atattaagtg
      901 gaagtattcg tgtacatgat aaaacattac aggaaaaaat atttaccatt ttaggattta
      961 gtgaagcaga aaaagaaaaa cgtttcgggt ttatggtgaa tgcatttaaa tacggtgctc
     1021 cgcctcatgg tggttgtgca tttggtttcg acagatgggt gatgttaatg gccggtggtg
     1081 atactatcag agatgtaatt ccgttcccta aaaataataa tggccgcgat atgatgatgg
     1141 atgcgccagg cgatgtggat aatgagcaat taaaaattgt gggtgtaaaa ttgttgtaag
     1201 cccgtttcac gcggagagtg gagaggcgga gattggttag ttgtgtttct cgcgaaggcc
     1261 caaaggcgaa atttgattgg tgaaaaacca gattcaagca agcaaagctg gatgtggtcc
     1321 tctgtaattg aattttgcag atttttattt aaatctttac cgacgcatta attttcactt
     1381 tattgtcatg tgctttttag ttccaagcca tttacaattc gcttaatacc gtgttttatt
     1441 aaggctacat taaaattgat aatgaggcct aacttaattc cggtaacttt taagtaggta
     1501 tttacttgtt taaaatgcac atctgcaaga gcctcaatcg acttcacttc aattaataat
     1561 ttattattga taatcaaatc agcacgaaaa gcatacttgt ttactctttc attatcccaa
     1621 tataagtaga ttgctttttg tctttccact tgcaaaccac attttacaag ctctttgaac
     1681 agtatctctt catatacaga ttcatatagc cccggtccta attttacatg tatttcaaat
     1741 gcaatattaa ctaccaactt tgctaattca ttctcatcaa gttccaccat aagtatttta
     1801 ttttgtgcga aactaaatcg aagatgaaat atataaaaat attaaccgcg tatactcgct
     1861 ttgagcggat ataaaaaaat gttttcgcgg actacctttg cgtgcttgaa tatttacata
     1921 atacatcaag cacctatata attaaatcaa cctttgggcc tttgcgagaa acctacgcat
     1981 ttataaacat caaactctta tctatatcaa gatgtaaaaa cctgtcagat tcattaaaag
     2041 ataccactgc tctaaacgct gtataaaaat cctcaatcgc ttttgatgat ttgagtggcc
     2101 gtctgaattc aaatgcttgt gcagctgtta gcaattcgat tgcaagaatt ttttcaatat
     2161 tttcgacaac acgtaaacat tttgtcgccg cattggcacc catactcaca tgatcttcct
     2221 gttcgttgca gcttgaaata ctatcaacac ttgcaggtgt acacaattgt ttattttgag
     2281 aaacaatact cgccgcagta tattgaggaa tcattaatcc cgaatttaat cccggatttg
     2341 gtgttaaaaa ttcaggtaga tttcttgccc cactcaataa acgatacaca cgacgttcag
     2401 caatattacc taattcgctt aatgccattg ccagaaaatc taaagtcaat gctaaaggct
     2461 caccatgaaa atttccacca ctcacaatta aatcatcctc aacaaaaata ttaggattat
     2521 cagtaacacc attaatttct ttttctacaa tcgatttaca ataatcaatt gcatcgcgac
     2581 atgccccgtg aacttgtgga atacatctaa aagaatatgg atcctgcact tgttgttttt
     2641 ccgcaaaaaa tgtttcgctg ccttcaagta aatccataat ttcttttgca gtgataattt
     2701 gtccgctctg atttcttaca cgatgaattt gcggaaaaaa aggctccgct ttacaatcgt
     2761 aagcttctaa tgataatgca gcaattttat tcgacttatg aataatgttt tgcgatttaa
     2821 ttaaattata caccgcataa ctactcataa attgtgtgcc attcaaaagc gctaagcctt
     2881 cttttgattg taatttgagt gcagataatt taaagtcttt caacacttca gcagttggtc
     2941 gaatttcgcc attatattta acactacctt ctccaattaa tgccaaactc atatgtgaaa
     3001 gcggacacaa atcgccactt gcgccaagtg aaccctgctc ataaattacc ggtaaaattt
     3061 cgaggttata aaaatcaatt aatcgccata ccgtttccac actaacacct gaattaccat
     3121 aactcaaact ttggatttta agtaacaata atattttgca aatttcatca ggaataacag
     3181 gtcctacgcc acatgcatga cttctaatta aattatactg taattgctcg aggtcatcat
     3241 cagaaatttt caccttacat aaacttccaa atccggtatt taccccataa acagccttac
     3301 cactgtcggc aatcttttta tcgaggtaag ttctgcataa ttgaattttt tctctagccg
     3361 tgtcgtccag tgccagtttt gccttcgatt ttataatggt atctattaaa gtaatagaca
     3421 gtggttgtga acctatatga taagtatcca taaacaattg tttctgcggc aaatttaaat
     3481 ttaaatttgc aacatgggcg atatcatgac cttttcgcag gtaaacaaac gattttcgga
     3541 tgttcaggcc ttgcaggatg taagttttac aataccggaa aacaccatat ttgggctttt
     3601 agggcctaat ggtgccggta aaacaaccct tatccggatt atcaccaaaa tatttgctgc
     3661 agattcgggg cacattgttt ttgatggttc cgacatccaa acccagcaat atgccaatat
     3721 tggatacatg ccagaagaaa aagggatgta taaaaaaatg aaagtgggtg aacatctcgt
     3781 gtatttgggc aggttaaaag gattaaaaaa atcagcagct caggaacgct caaaatattg
     3841 gcttgaaaaa ttaggcgccg gcgactggtg gaataaaaaa ctcgaggatt tgtcgaaagg
     3901 aatgcaacaa aaaacacaat ttattgccac cgttttacat aacccgaaat tattaatttt
     3961 agatgagcca ttttccggtc ttgaccctgt gaatgcggaa atgattaaaa atgaaattta
     4021 tcaattacat caacaaggaa caaccatcat tttttccaca cacagaatgg aacaggtaga
     4081 agaaatttgt aaaaccatag cacttatcaa taaagggaaa ctcattttaa acggagaagt
     4141 agcaacaata aaacaacaat ttaaacaaca taaattttta atcagttttg aatcgcttcc
     4201 tgaaattgaa gataatgatt tgtttacagt agaccagatt aatggccaaa gtgttaccgt
     4261 tcaactaaaa tcaaaaaacg accaaaatca ggttctacaa tattttataa gtcgcaatgc
     4321 cattattaat gcgtattatg aaatattgcc atcgctcaac gatattttta tctcaattgt
     4381 aaataaggct aaccatgaat aaaacattgc ttataatcca acgggaatat attacccgtg
     4441 tgagaaaaaa atcgtttatc attttcacac tgttaggacc tgtgtttttt ctgctgatta
     4501 gtttgttgcc cattctaatt gctaatgcaa ataaaaacaa tctgcatata ttggtaaaag
     4561 acgatagcgg attaattccg gcactacccg atactgccgg cttatatttt acgtataaat
     4621 atcaatccat tcctgtcaac accttaaaaa cagattacag caccattgga gaaggttacg
     4681 acgcattttt atatatacct gccatgcaac cggatgcacc ttatggcatt gaaatttata
     4741 gtaacgaaca agtgagttta actactaaat tgtatatcga aagtattatt gcagataaac
     4801 tggaaaccat caatttacaa cagcacgatt tagataaaaa tatcttactc aaattgagac
     4861 cgaaagtaga tattgatgat aaagtaactt ccggcgaatc acaaaaagaa ggtaatgcag
     4921 caattgccag tgcattcggt tttggcatgg gatttatcat ttatattgta ctgctcattt
     4981 acggcactat ggtgatgcgg ggtgtaatgg aagaaaaatc atcacgtatt atcgaagtga
     5041 ttatttccag tgtgaaacct tttcagttaa tgatggggaa aatagttgga attggtttag
     5101 ttgggcttac acaattcgta gcctggggat ttttaatcac tatcttaaat attataatcg
     5161 gaattctttt tgcgggacaa ctcagcgatt tacaacaagt gagcgcatca agtggtgagg
     5221 ttgaaatgaa tgctataaca gaagctatgc aaagcatgca aaacataccc ataggttatt
     5281 atttaagtat gtttattatt tactttttag gtggctattt tatttatgct tcattgtttg
     5341 ctgcaatagg ttcccttgcc ggcgatgaag agggcgatgt acaaacattt gcattcccgg
     5401 taaccatgtt aattttggtt tccattttta ttatgatggc ggtagtgcaa caaccgcata
     5461 caccgcttgc attttgggca tctattattc cgttgtcatc accaattgta atgcctgcat
     5521 taattccatt tggtgttccg acatggcaaa ttgttttatc aattgtattg ttatttggcg
     5581 gatttatttt taccaccttt ttggcaggac gtatttacag aactgccata ttaatgtatg
     5641 gtaaaaaaat taaggtgaaa gaagtattta agtggatgtt ttataaataa acttagcgca
     5701 aaatcaataa cctatctgcg tctaaacgaa gcataataaa tactaatata gaaaacgcga
     5761 gcacagatga acctccgtat gaaataaacg gaagtggtat accgattaca ggaatcagac
     5821 cgatggtcat tcctacattt atcataattt gtaaaaagaa aaagctggca acacagtagg
     5881 catataccct tgaaaatggt gagcgctgac gttcggcaat aaaaataatg cgcagcaaaa
     5941 acaacaaata cacaaccaga aaaacaaatg tcccaagaaa tccgaactcc tctcctatgg
     6001 tgcaaaaaat aaagtctgta ctttgttccg gcacctgttc cgattgtgtt aatgttccct
     6061 gcaaataacc tttacctgca acaccacctg aaccaatcgt cattttactt tgtgttacat
     6121 tatattcagc atcggcatta tccgacatgc ctaataatgt ttcaattctt tg
//
LOCUS       DAORKW010000179         6162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_465_length_6162_cov_4.959063, whole genome shotgun sequence.
ACCESSION   DAORKW010000179 DAORKW010000000
VERSION     DAORKW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_465_length_6162_cov_4.959063"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLB46_08350"
     CDS             <1..263
                     /locus_tag="PLB46_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76568.1"
                     /translation="CTGQGFTVQVLKEEGYTVRFATKEGIENDADVMSGFKFLLIDMS
                     AAAFNGDPITVDLCDETLNTKVTITHAEAKSFMEKMLTEQPL"
     gene            complement(665..1006)
                     /gene="ssb"
                     /locus_tag="PLB46_08355"
     CDS             complement(665..1006)
                     /gene="ssb"
                     /locus_tag="PLB46_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRF76569.1"
                     /translation="MNNLRNKVQLIGRLGADPEVKSGAGYGKLAKFNLATDESYKNAK
                     GEKVKETQWHTLTAWGKTADFVEKYLTKGIEVVVEGKLINRNYTDKSDVKRYVTEIHV
                     SDLVIVSHGKK"
     gene            1703..2230
                     /locus_tag="PLB46_08360"
     CDS             1703..2230
                     /locus_tag="PLB46_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF76570.1"
                     /translation="MSEAFLITDRLLLREFTETDIDLIYKLDSNPKVMEFIRPPTKTL
                     EEANATYQKILNTRKKDIRFGNWIGVTKSNNQPIGWFCLKDLDNTSTIEVGFRLLPEF
                     WGNGYATEGAKALIKYGFEQCNLPFIAGVTKPENIRSKNALQKCGLKFLRNDYIYETQ
                     VHYFEISVNDYNKNQ"
     gene            complement(2248..3171)
                     /locus_tag="PLB46_08365"
     CDS             complement(2248..3171)
                     /locus_tag="PLB46_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76571.1"
                     /translation="MKLSVRHKNSLLLTLVLLFSAMRSQAAEIKTIYTWTYDENLYTM
                     VYYFDQNTYNYYKGQDRVYNNFLFYLNESARFPVIEQFANEFKLLAAENKLSDWQLVE
                     CVVAFVQTMKYKNDGTYEYPRYPIETLVEKGGDCEDTAILLEAILKELGFDCVLVSPT
                     KHMGVGIALKYEVNGIAFQHRNKFYYYIETTSAGWGIGDYPDHLSKDVSIYDPGDVDG
                     GKLLSAYSYTYKNYTIPDDSEVAEPVYAQEEETKSTNDYEFYDDFDFEPTPENSYTID
                     VDQVVIDGEKETVVTKKVASGGQEKIVATSK"
     gene            complement(3346..4899)
                     /locus_tag="PLB46_08370"
     CDS             complement(3346..4899)
                     /locus_tag="PLB46_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRF76572.1"
                     /translation="MQLVKTFGSSVHGVNAFTVTIEVNVSVSGAGADIKYFMVGLPDN
                     SIRESWHRIESAVKENHFNMPRTKIVVNLSPADVRKEGTHYDLPIAIGVLGSSLQMPS
                     HHFHEYVILGELSLDGSIKPIKGALPIAIQARKEGFKGIILPKQNAREAAIVSDIKVY
                     GVENIYEVVRFLSDDLDLEETVVNTREEFFNQVGFNDIDFSDVKGQENIKRALEIAAA
                     GGHNAILIGPPGAGKTMLAKRLPGILPPLSLYEALETTKIHSVAGKLPVGSALMYARP
                     FRNPHHTVSDVALVGGGNNAQPGEISFAHNGVLFLDELPEFKRNVLEVLRQPMEERRV
                     TISRARFSVEYPANFMLLAAMNPCPCGYYNHPDKECVCAPGVVQKYLNRISGPLLDRI
                     DLHVEVVPVGVKELSQLGDGEQSNSIRERVIAARKIQEQRFEGTSIHSNAQMSSKQVK
                     ELCIITTAGQNLLNKAMDKLGLSARAYDRILKVSRTIADLSGSNDILIEHLAEAIQYR
                     SLDRDNWAQ"
     gene            complement(5034..5531)
                     /locus_tag="PLB46_08375"
     CDS             complement(5034..5531)
                     /locus_tag="PLB46_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76573.1"
                     /translation="MKNLFTALFALLVTLTNAQIIDIDKPETYFPTGFGNLKLGIGIQ
                     DFQKIMDTTKMERNATSAFTFIQFSKVNPANNIASIQLKFDSPQQGVNTNRPLYEVTF
                     EFNDKELADKFATEKFTGQYRITDKADKEWFLSTTKDYWMIVRKSGTTVTLAAMMNGT
                     EWGFE"
     gene            complement(5676..5787)
                     /gene="rrf"
                     /locus_tag="PLB46_08380"
     rRNA            complement(5676..5787)
                     /gene="rrf"
                     /locus_tag="PLB46_08380"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(5874..>6162)
                     /locus_tag="PLB46_08385"
     rRNA            complement(5874..>6162)
                     /locus_tag="PLB46_08385"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1929 a         1249 c         1032 g         1952 t
ORIGIN      
        1 actgtaccgg ccagggattt accgttcaag tgttgaaaga agaaggttat actgttcgtt
       61 ttgctaccaa agaaggcatt gagaacgatg cagatgtgat gagtggtttt aaatttcttc
      121 ttattgacat gagcgccgct gcttttaatg gcgaccctat aacggttgac ctctgtgatg
      181 aaacactcaa taccaaggtt accataacgc atgctgaggc aaaaagtttt atggaaaaaa
      241 tgttaactga acaaccatta taacagctat tcattgctta aagcacttag ctcatcataa
      301 ccatataaca ggttgtcagc cacaatttac ctgatgctta atcaacccaa tattgtgctc
      361 tgattatttg aacattaaaa aggttgacac ttgcatgttt attatacaat ggcatgctgt
      421 ccgttcaaca accctaaata tggcttgtaa agcaatagta atttaacaca ggtaaattcc
      481 caataatgct tgttgcgaac agccggtaaa acccataatc ggattatcag ctaatcgcca
      541 aaatcaccat tctaccacca accaaaatcg cagtaaaact gcccctagtt cccctaacca
      601 acataagcaa aaaccggcac ctcacaaata aaggaagttc gaggcagttt gtattacggg
      661 aatgttactt tttaccatga ctaacgatga ccaggtcgct aacatgtatt tctgtaacat
      721 accttttaac atccgatttg tctgtgtaat tgcggttaat gagtttaccc tctactacca
      781 cttcaatacc ttttgtgaga tacttttcga caaagtctgc cgttttgccc caggccgtaa
      841 gcgtatgcca ttgggtttct tttacttttt ctcctttggc atttttataa ctttcgtcag
      901 ttgccaaatt aaatttcgca agttttccat aacctgctcc ggattttact tccgggtccg
      961 cacctaagcg gccgattaac tgcaccttgt ttctaaggtt gttcatacgt caatttttta
     1021 agggttaaac aatcagcgtt tttaatagcc gtgttattat tatttaataa caacggttca
     1081 aagatgaata ccgctaatca aatgacaaaa ttttaaccgc ttatattcgg atatactcgc
     1141 ttcatttcgt tttttcccaa ttatttatat atttgcctca tgttatttat ccaaaacctt
     1201 aaaaacttta aaatcttgtt tatctatgga caacaaacat tgccccgaat gcggcaagac
     1261 cttatttggt cgtgtcgaca aaaaattttg ctgtgatgta tgccgacatg cacataacaa
     1321 caggcagcga acattaaatg gtgtctattt gaattccgta aattcaatcc ttcgaaaaaa
     1381 ccggaacatt cttgccaact ttaaacccgc agaaccggtt aaaactctta aacaaaagct
     1441 gtatgaaaag ggatttaatt tcgcttattt tacatctatc tctacaaaca aaaaaggtat
     1501 aattacctat tattgttatg actatggtta catattaagt gaaaaagata ttgtacatat
     1561 cattcgcaaa gaggatttgt agattaattt gttttatgca ataaattatt tgtgcaattt
     1621 ttaattaaat gcgcactggt tcttttttta tagattatgg ctgaatcgtt atctttgaaa
     1681 accaagcatc aatatatcga ttatgtcgga agccttttta attacagacc gccttttatt
     1741 gcgagaattt acggaaacag atattgattt aatttacaag ttggacagca atccaaaagt
     1801 gatggaattt attcgccctc caacaaaaac tttggaggag gctaatgcta cctatcaaaa
     1861 aatattgaat acccggaaaa aagatatccg ttttggcaat tggattgggg ttacaaaatc
     1921 aaataatcaa cccattgggt ggttttgtct aaaagacctt gacaatactt ctactataga
     1981 agtcggattc agactattac ctgaattttg gggaaatgga tacgcaaccg aaggcgccaa
     2041 agcacttatc aaatatggct ttgagcagtg taatttaccg tttatagccg gtgttactaa
     2101 accggaaaat atacgctcta aaaatgcctt acaaaaatgc ggattgaaat ttcttcgcaa
     2161 cgattatatt tatgaaacgc aggtgcacta ctttgaaata tcagtaaatg attataataa
     2221 aaatcaataa atagtaatca gcaattttta tttggaagta gctacaattt tttcttgtcc
     2281 gccggaagct acttttttag taacaactgt ttctttttca ccatcaataa caacctgatc
     2341 aacatctatt gtataactat tttccggtgt tggctcgaaa tcaaaatcat cataaaactc
     2401 gtagtcatta gttgattttg tttcttcctc ctgagcataa acaggttcag caacttctga
     2461 gtcatcagga atcgtataat ttttataggt ataactataa gccgataata attttccacc
     2521 atccacatca cccgggtcgt aaatacttac atctttactt aaatggtcag ggtaatcacc
     2581 tattccccat cctgcacttg tagtttctat ataataataa aatttgtttc tgtgttgaaa
     2641 tgcaatgccg tttacttcat attttaatgc aattccaaca cccatatgtt tagttggaga
     2701 aactaacaca caatcgaatc caagttcttt taatattgct tccaataaaa ttgccgtgtc
     2761 ttcacagtct ccaccttttt caaccaatgt ttctattgga taccgcggat attcgtaagt
     2821 gccatcgttt ttatatttca tggtttgtac aaaagccaca acacactcca ctaattgcca
     2881 atcactcaat ttattttcag cggccaacaa tttaaactca tttgcaaatt gttcaattac
     2941 cggaaaacga gccgactcat ttaaataaaa taagaagtta ttatataccc tgtcctgtcc
     3001 cttataatag ttatatgtat tctggtcaaa ataataaacc atggtatata aattctcatc
     3061 ataagtccat gtgtaaatag tttttatttc agctgcctgt gaccgcatag cagaaaataa
     3121 caataccaat gttagtagca gtgaattttt atgcctgaca gatagtttca tacattaggt
     3181 tttgcgtata ccttatttca tactgtggtt ttatcaataa acttgccaca gatgaaattt
     3241 attgttttag gtgcacgata gttttacaca tcccaaatat attaaaccct gaaccaagtt
     3301 ctaaaatcat attacttaaa aacgtggaaa accaagcaag gtgctttact gtgcccagtt
     3361 atcacggtct aaactccggt attgtattgc ctctgcaagg tgttcaataa gaatatcatt
     3421 tgatcctgac aggtcggcaa tagtcctgct tacctttaaa attctatcat aggccctggc
     3481 acttaaccct aatttgtcca ttgctttatt caacaggttt tgaccggcag ttgtaataat
     3541 acataactct tttacctgtt tcgaactcat ttgcgcatta ctgtgaatag atgttccttc
     3601 aaaccgttgt tcctgaattt tcctcgcagc aataacacgt tcacgaatac tattactttg
     3661 ttcaccatca cccaattgcg ataattcctt tactcctaca ggaacaactt caacatgtaa
     3721 atctattcgg tcgagtagtg gtccggaaat cctatttaaa tatttctgga caaccccggg
     3781 cgcgcaaaca cattccttat ccgggtgatt ataatatccg caagggcaag gattcatggc
     3841 agctagtaac ataaaatttg ccggatattc aacagagaat cttgctcgtg aaatagtaac
     3901 ccttcgttct tccatcggtt gccgaagcac ttccaaaaca ttccttttaa attcgggaag
     3961 ttcatctaaa aaaagtacgc cattatgagc aaatgatatt tcacctggct gggcattatt
     4021 accgccacca actaaagcaa cgtcgctcac cgtgtgatga ggatttctaa atggtcgtgc
     4081 atacatgagc gcactaccaa ccggtaattt tcctgcaacg ctgtgaattt ttgtagtttc
     4141 taatgcttca tataaactca atggaggcaa aatgccgggt aatcgttttg ccaacattgt
     4201 tttacccgca ccgggaggac caatcaatat agcattatgc ccaccggcag ctgcaatttc
     4261 gagggcgcgt ttaatatttt cctgtccttt gacatctgaa aagtctatat cattaaatcc
     4321 gacctggtta aaaaattctt cgcgggtatt tacgacagtt tcctccaaat caaggtcatc
     4381 gcttaaaaat cttaccactt cataaatatt ttctaccccg tacactttta tatccgatac
     4441 aattgcagcc tctcgggcat tttgtttagg tagaatgata cctttgaaac cttctttacg
     4501 ggcttgaatg gcaattggta aagccccttt aattggtttg atagaaccgt ctaacgacaa
     4561 ttctcccaga attacatatt catgaaaatg atgtgaaggc atttggagtg aagaacctaa
     4621 tactccaata gctattggca aatcgtaatg cgtgccttct tttcttacat cagcgggtga
     4681 aagattcacg actatttttg ttcgcggcat attaaaatga ttctctttca cagccgattc
     4741 gatgcggtgc caactttcgc gaatagaatt atccggaagt cccaccataa aatattttat
     4801 atctgctccg gcaccggaaa cagaaacgtt aacttcaata gttacagtaa atgcattaac
     4861 gccatgcaca gaactgccaa atgtttttac aagttgcata cattgttttt aaggatgaac
     4921 aaaattgagt ttaccctata aatttcactt tgatgatttg ccaacaaggc ataacaaggc
     4981 aagtagtaat taaaggataa attcagttgt aaaaatacta caaggaaaat aaattattca
     5041 aatccccatt ctgtaccatt catcatagca gcaagtgtaa cagtggtgcc ggatttgcga
     5101 acaatcatcc aataatcctt agtagtagaa aggaaccatt ctttatctgc tttatcagta
     5161 attcggtatt gaccggtaaa tttttcggtt gcgaatttat cagcaagttc tttatcatta
     5221 aattcgaaag taacttcata taaaggtcga ttagtattta caccttgttg aggagaatca
     5281 aattttaatt gaattgaagc aatattattt gccggattta ctttcgaaaa ctggataaaa
     5341 gtgaatgctg aagttgcatt tctttccatt ttagtggtat ccataatttt ttgaaaatcc
     5401 tgaataccga tacctaattt taaattgcca aaacctgtgg ggaaataagt ttcaggttta
     5461 tcaatatcga taatttgtgc atttgtcagc gtaactaaca aggcaaaaag tgcagtaaac
     5521 aggtttttca tatgcgtaat tctcctatca aatataagaa agagcaatta aacaagtggg
     5581 agaagaaaaa atattttcac ctggcatgaa tatcaacctg aacaacggaa gtatggtaca
     5641 ataaaaaacc ccgaccgaag tcagggtttt tataaacttg gcaacgacat actctcccag
     5701 attttactca agtaccatct gcgctgtagg gcttaacttc tctgttcgga atgggaagag
     5761 gtggacacct acgctatagt caccaaagac tctttaacgc tagaactgcc gaagcatgtt
     5821 cgaggtcata ataattttga cgtattgaat ggggtgtaat taattaataa aaaaaaatga
     5881 aagcttacgg gtaattagta ctgctcggct ttgccattac tgactttaca cctgcagcct
     5941 atcaacgtgg tcatcttcca cggcccttaa aagagatctc atcttgaggt aggcttcgca
     6001 cttagatgct ttcagtgctt atcctttccg aacatagcta ccctgcgatg cagctggcgc
     6061 cacaacaggt acactagagg ttcgtccaac acggtcctct cgtactagag tcaggtcctc
     6121 tcaaatctct aacgcccaca acagataggg accgaactgt ct
//
LOCUS       DAORKW010000180         6092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_480_length_6092_cov_4.068080, whole genome shotgun sequence.
ACCESSION   DAORKW010000180 DAORKW010000000
VERSION     DAORKW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_480_length_6092_cov_4.068080"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PLB46_08390"
     CDS             complement(<1..905)
                     /locus_tag="PLB46_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76574.1"
                     /translation="MQLYAITSLSIAFWGLQLAAQTAKEPEKSFADKFELHGYGEMTF
                     NVFDWETYPDKRNNIDLNRIVFEPEYAFSEKVKLEMEIEFEHGGTGATMEFDKFEEFG
                     EFEMEIEKGGEVIIEELEIAWQAKPWLELKAGRISVPVGHIAAYGEPLDYMTSTYNNM
                     EANLIPAAWYENGIGAEIEWKKLAFELALISGLDNSAFSSANWIQRGNQTRFEYVNAE
                     AFALAARLDYVVNDNVIFGVSSYTGNSTPNRPKPDITSDGVVQIVDAHLHAQFSAFTF
                     NAMFLSGTLQNAATISEANRNLSNNL"
     gene            complement(1070..2341)
                     /locus_tag="PLB46_08395"
     CDS             complement(1070..2341)
                     /locus_tag="PLB46_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76575.1"
                     /translation="MQEFLIPALWATILLFFIYRFDFFKIQEINPAWPYAAFLFKLLL
                     GFANYYIWLNIIGHGDSLRYFHDSQIVYQSLFDNPTYFFELITRSSLENIPAHLIPYQ
                     KALYIEWHVNEYHMVRLLAILNVFTFGNVWANIVLIAFGSFAATLSLYKSLNRFLVID
                     ADKKNRIFLFLFFLPSIVFWTGGLLKEAPVFMLLCIIIIQLFTIENSPNRSKTPAKPL
                     LILIVCIYVLFLIRDYLAILVSLNLLLLFFARTIVKPEYLNKTFIINTITVIVLFFAV
                     PIIFPQLNYFNYAQSEQTYFLNGAPDPDYTFAAIGGDGIAVIQQIPYALNNIFFRPNL
                     LHSDSVFRLYQSLELMIVWLIIVFLFIKMLRQKQPFSTTALFMIIVGLELLGIYGLMV
                     TDADTLSRYRSIPLFFILLFMWLHKPKHTTV"
     gene            complement(2376..3968)
                     /locus_tag="PLB46_08400"
     CDS             complement(2376..3968)
                     /locus_tag="PLB46_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76576.1"
                     /translation="MYFKSTAIANLLRNTITTLVLFTFTMHVSFGQGVQIDNSFGEEG
                     FFKFSFQDNYVIGEAAMIQKDNKIIIAGYNNTDRQVLLVRTTENGLLDSVYGENGVAT
                     ISGNNNLIIEDMNVATICEDESIIVGLSCYPVSDYYPVEATLIKLNKLGQIDSSFGDN
                     GFAIQFVSEMNFTATINDILIQSDNKILFCGHYFDIGSDDINHFFVGRCLPDGSPDPS
                     FGKNGIVTEAFDGSPCAAQTIYRQHDGKILVGGKLNNDFAIARYDQSGKLDETFANGG
                     KLVTNVANAMALNAIAELKDHTIILAGFGLYYGYQDCSPYYRNILIGLTQDGVLSGNF
                     SETGNIQYLTGNCVSVANDILIQSDNKILVGGSGGKATGNGLYNLTISRLTENGLLDE
                     SIGGEGDQDFISGLDKNYCVALSQQSDGKLIMAGYGYDDSHYYLIAMRADAGLIPPGH
                     TLVEHEQAEIWSYDKTIYLTNKAAEDVKCNLVNALGQIIFTFTAPPGVSTYNLPELPD
                     GWYVLSLNMPYGLVTHKLLFTN"
     gene            complement(3975..5936)
                     /locus_tag="PLB46_08405"
     CDS             complement(3975..5936)
                     /locus_tag="PLB46_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76577.1"
                     /translation="MHTNKYKKLIAYSSAALGFIALNNDISAQVIYTNIDPDENLGLS
                     GQLQLDINDDGVIDIGLDAYLAQWSSYSYWHGWSTFTSGEYFYASNFSAVVGTPLNPN
                     LAANLQPGDIIGASAIWNTNNEINFAGLNLNTYSYVEIIEGWNKQNNYIGFKFQADGQ
                     THYGWVRLSIDNLSNNVAELPKLIIQDYAYEATPNTPITIEHVTASIAENLVLKDIGE
                     TSTANDLQLTFNKAADESFVSEYRVFLHRIPDAIYNSLDALLALPADKYISIIPDGTN
                     HVINFDATTLDTDGNPIASNSTYEARVVSMPDGIQTNIANVAVVSNKEEIIIRSTGEA
                     QLIAILKNGSTGTSSDFKVTFKNPSIEYGIAAFRVYIIKYGLNVDYDTLINLDENYFV
                     EIPYSTESNYVVPLPPEIKTIGDDGPIIFESYTAAVLTIPDSIAATTGELNFSKHSLA
                     VSSPLYPEMPVVLLDDTTKTPHDILVNFPMSPVEEKINDYKIILVPAEDTATFTVEDV
                     LDLSANNYYQVMPVGADINTHLPSFLNDSHGYTPNPETIYVVYVALTSVYYSSNVSLS
                     FPSIPFTLKDGKVEGVPVLTFYNNAIYYNAPANEVQNILLFDIGGRKIAEWAVSGSGI
                     INNFDGIAPGIYFLQYNNADVIKIFIDKQ"
BASE COUNT         1983 a         1122 c         1077 g         1910 t
ORIGIN      
        1 agattattgg ataaatttct atttgcttct gaaatagtag cggcattttg caaggtgccg
       61 ctcaaaaaca tggcattaaa agtgaaagct gaaaattgtg catgtaaatg tgcatctact
      121 atttgcacaa cgccatcgga agtgatatcc ggttttggtc tgtttggggt gctatttcct
      181 gtatatgaac ttacgccaaa aattacatta tcgtttacta catagtctaa cctcgcggct
      241 aaggcaaaag cttcagcatt cacatattca aaacgtgtct gattgccgcg ttgaatccag
      301 tttgccgaac taaatgcaga gttgtctaat ccgctgataa gggctaattc aaaagccaac
      361 tttttccatt caatctctgc accaatgcca ttttcgtacc atgcagcagg aattaaattg
      421 gcttccatat tattataagt ggaagtcata taatcgagtg gttcaccata agccgcaata
      481 tgaccaaccg gaacggatat tcggcccgct ttgagttcga gccagggttt tgcctgccag
      541 gctatctcca attcctcaat aattacttca ccaccttttt ctatttccat ttcaaattca
      601 ccaaattctt caaacttgtc aaattccatg gtagcgcctg ttccaccgtg ttcaaattca
      661 atttccattt ccagtttcac cttttcggaa aaagcatatt ccggttcaaa tactatgcgg
      721 tttaggtcga tgttgttgcg tttgtcaggg tacgtttccc agtcgaacac gttaaatgtc
      781 atctccccat aaccatgcag ttcaaacttg tcggcaaatg atttttcagg ctctttggca
      841 gtctgtgccg caagctgtaa gccccaaaag gcaatggata aggaggttat ggcgtatagt
      901 tgcattattt agatttgttc taaacaacgc aaaagtaagt gcaggggagg gaacaaaaaa
      961 ggaatggtgt caaaataccc taattagggt aggtcgaaaa atgtgtagac atatatgtag
     1021 taacaagtat gaacaaacac tacaaaaaaa ttgacttatt ataaaaaaat taaactgtag
     1081 tgtgtttagg tttatgcagc cacataaaca ataaaataaa aaacagggga atacttctgt
     1141 agcgactcag ggtgtcggcg tcggtaacca ttaatccata tatacctaac aattcaagtc
     1201 caactataat cataaataaa gcggtggttg aaaatggctg tttttgtctc aacattttaa
     1261 tgaacagaaa aacaatgatt aaccagacaa tcattaattc caaactttgg tataatcgaa
     1321 aaacactatc gctatgtaat aaattgggcc gaaaaaaaat attattcaat gcgtaaggaa
     1381 tttgttgaat aacggcaatg ccatcaccac caatcgcagc aaaagtataa tcagggtccg
     1441 gcgctccatt taagaaataa gtttgttcag attgtgcgta attaaaataa ttcagctgcg
     1501 gaaatatgat tggaacggca aaaaataaaa cgataaccgt gatggtattg atgataaatg
     1561 ttttattgag gtattccggt tttacaatag ttctggcaaa aaacaatagt aataaattta
     1621 agctgaccaa aattgccagg tagtcgcgga ttaaaaacaa aacataaata cacactatca
     1681 aaatcaacag aggtttcgcc ggtgttttgc tgcggttggg gctgttttca atagtaaaca
     1741 attgaataat aatgatacat aacaacataa acaccggagc ctctttgagt agtccgcctg
     1801 tccagaaaac gattgaaggt aaaaaaaata ggaacaggaa aatccggttt tttttgtcgg
     1861 catcaataac aagaaaacga tttaaagatt tgtataaact caaagttgcg gcgaaactgc
     1921 caaatgcaat taaaacgata tttgcccaaa cattcccaaa tgtaaacaca tttaaaatag
     1981 ccagtaaacg caccatatga tattcgttta catgccattc gatgtataag gctttttggt
     2041 aggggattaa atgagcaggt atattttcaa gtgagctgcg tgtgattaat tcaaaaaaat
     2101 aggtcggatt gtcgaataaa ctttgataaa caatttgact atcgtgaaaa tacctgagac
     2161 tatcgccgtg accaattata ttgagccaga tataataatt agcaaaaccc agcaagagtt
     2221 taaacaagaa agcagcataa ggccaggccg gatttatttc ttgtattttg aaaaaatcga
     2281 agcgataaat aaaaaatagt aaaatagttg cccagagtgc cggaatcagg aattcttgca
     2341 tggttcaaag atactgaata agctacatcc tcaattcaat tggtaaacaa tagtttatgt
     2401 gttactaaac cataaggcat attgagtgat aaaacatacc atccatcggg cagttctggt
     2461 aggttataag ttgatacacc tggcggagcg gtaaatgtaa aaataatctg accaagtgca
     2521 ttcacaagat tacatttcac gtcttcagca gctttattgg ttaaatatat tgttttatca
     2581 taactccaaa tttcagcttg ttcatgctca accaatgtat gtcccggtgg aattaatccg
     2641 gcatcggctc gcattgcaat taaataataa tgactatcgt cgtagccata acctgccatt
     2701 attagtttgc catcggattg ttgacttaaa gcaacacaat aatttttgtc caaaccggaa
     2761 ataaaatctt ggtcaccttc gccgcctatt gattcgtcta gtaaaccatt ttcagtcaat
     2821 ctggaaattg tcaaattata taatccattt ccggttgctt tacctccgga gcctcctacc
     2881 aatattttat tgtcagattg tatcagtata tcatttgcga ctgaaacaca gttgccggtt
     2941 aaatattgaa tatttcctgt ttctgaaaaa ttacctgata acacaccatc ctgtgttaac
     3001 ccaatcagaa tatttctata atagggagaa caatcctggt aaccataata caagccaaaa
     3061 cctgctaaaa ttatggtatg atcttttaac tcagcaattg catttaacgc catagcattt
     3121 gctacattgg taaccagttt acctccattt gcaaaagttt catccaactt gccggattga
     3181 tcatatctgg caattgcaaa atcattattt agtttgccgc ctactagtat tttaccatca
     3241 tgctgcctgt aaatggtttg tgctgcacat ggtgaaccat caaatgcttc ggttacaata
     3301 ccatttttgc caaagcttgg gtcaggactt ccatccggta aacatcggcc aacaaaaaaa
     3361 tgattaatat catcagagcc tatatcaaag taatggccac aaaataatat tttgttatcg
     3421 ctctgtatta aaatatcatt aatagttgcc gtaaaattca tctcagaaac aaattgtatg
     3481 gcaaaaccat tatcaccaaa actagaatca atttggccta atttatttaa cttaattaag
     3541 gtagcttcaa ccgggtaata atctgaaacg gggtaacaac ttaaacccac aataatactt
     3601 tcatcttcgc atattgttgc gacattcata tcttcaatta tgagattgtt atttccacta
     3661 attgtggcta caccattttc accatataca ctatcgagta atccattttc agtggtgcgg
     3721 acaagtaata cttgtctatc tgtgttattg tatcctgcaa tgattatttt attatctttt
     3781 tgaatcattg ctgcttcgcc tattacgtag ttgtcttgga atgaaaattt aaaaaatccc
     3841 tcttctccaa aactgttatc aatttgaaca ccttgaccaa aactaacatg catagtgaag
     3901 gtgaacaaca ccagggtggt aattgtgtta cgtaacagat ttgctattgc tgttgatttg
     3961 aaatacattt tggtttattg cttgtcgata aatattttaa taacgtcagc attattatat
     4021 tgtagaaaat atatgcccgg tgctattccg tcaaaattgt tgataattcc ggaaccggat
     4081 actgcccatt ctgcgatttt tcttccacca atgtcgaata ataaaatgtt ttgaacctca
     4141 ttagcaggtg cattatagta aatggcatta ttgtaaaaag taagcaccgg aactccttcc
     4201 actttcccgt cttttaatgt gaaaggaatg gatggaaagg ataaacttac attgctacta
     4261 taatagactg aggtaagtgc aacatacaca acatagattg tttccggatt tggtgtatat
     4321 ccatgcgagt cattaagaaa agatgggagg tgggtattga tatcggcacc tacaggcatt
     4381 acttgatagt aattattcgc agataaatct aatacatctt ctactgtaaa tgtcgccgta
     4441 tcctctgcag gtactaatat aattttataa tcgtttattt tttcttcaac cggcgacatt
     4501 ggaaaattga ccaagatgtc atgtggtgtt ttagttgtat catctaacaa aacaacgggc
     4561 atttccgggt aaagtggtga tgaaacagca agtgaatgtt tactgaaatt caattcaccg
     4621 gtggttgcag ctatagaatc aggaatggtt aatacagctg cagtatatga ttcaaaaatt
     4681 ataggaccat catctcctat ggtttttatt tctggtggta atggaaccac ataattagat
     4741 tcggttgaat atggaatttc aacaaaataa ttttcatcta aatttattag ggtatcataa
     4801 tcaacattta atccatattt tattatataa acacgaaaag cagcaatacc atattcgata
     4861 gaagggtttt taaaagtaac tttaaaatca cttgaggtac ctgtggatcc atttttcaaa
     4921 atcgcaatca attgagcctc tccggttgaa cggataatga tttcttcttt attacttacc
     4981 acagcaacat tggcaatatt tgtttgaata ccatcgggca ttgacactac tctggcttca
     5041 taagtggaat ttgaagcaat aggattccca tctgtatcaa gtgttgttgc gtcaaaattt
     5101 attacatgat tcgtcccgtc aggaataata ctgatatact tatcggcagg taacgcaagt
     5161 aatgcatcta aactattata aatcgcatca ggaattcggt gcaggaacac ccggtattca
     5221 ctaacaaatg attcatcagc ggctttatta aaggttaatt gtaaatcatt agcagttgaa
     5281 gtttcaccaa tatctttcaa cactaaattt tctgcaatcg aagctgtaac gtgctcaata
     5341 gtaataggcg tgttaggtgt ggcttcatag gcataatctt gaattataag tttaggaagt
     5401 tctgcaacat tattggataa attgtcgata cttaatcgca cccaaccata atgagtttgg
     5461 ccatcagcct gaaatttaaa tcctatataa ttattttgtt tgttccagcc ttcaataatt
     5521 tctacataac tgtaggtatt tagattaagt ccggcgaagt taatttcatt gttggtattc
     5581 caaatagcac ttgccccaat aatatctccg ggctgtaagt tagcagctaa attggggttt
     5641 aatggtgttc caactactgc ggaaaaatta ctggcataga aatattcacc ggaggtaaat
     5701 gttgaccacc catgccaata gctgtatgac gaccactgag ccaaataagc atcaagtcca
     5761 atatcaatta caccatcgtc gttgatatct aactgtaatt ggccagataa acccagattt
     5821 tcatcagggt caatgttggt gtatattact tgggctgaaa tatcattatt taaagcaata
     5881 aagccaagcg cggcagaaga gtaggcaatt aattttttat atttatttgt atgcatttta
     5941 ttgctggtta ttttatgaca gtgatatttc ctttaagttg cgtttttatt ccacttaaat
     6001 actcaatttc tgcagtataa atatacacac cttcctgcac ttctgtacca ttaaacttgc
     6061 catcccagcc ttgatttgtt gactcaggtg tg
//
LOCUS       DAORKW010000181         6069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_485_length_6069_cov_4.022946, whole genome shotgun sequence.
ACCESSION   DAORKW010000181 DAORKW010000000
VERSION     DAORKW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_485_length_6069_cov_4.022946"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..2142)
                     /locus_tag="PLB46_08410"
     CDS             complement(10..2142)
                     /locus_tag="PLB46_08410"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRF76578.1"
                     /translation="MSNLLFAQNIAINTTGTVADASAMLDVTSTTKGVLIPRMTTAQR
                     TAIVSPATGLLVYDNTQSAFYYFDGSVWKPLINNSTGWSTTGNAGTTPGTHFLGTTDD
                     KALIFKTNNTPSGLISTGENLFLGYNAGRNDISINNVGIGSNAMYLNTTGNYNTALGF
                     QALFSNVAGNENTGIGYKCLYFNTGSSNTGIGYNVMYDNTTGNYNTALGALALENNTT
                     GASNTGIGYKSLSTITTASNNTAIGYQSGKSLGTGTENVAIGAFALETGSTASETVAI
                     GYEALRFNTASNNTAIGSGALSSAVTGGYNVAVGNGGLKSTTSGIENVAIGFYSLYDN
                     TTGSGNTALGAQSAANITASSYNLALGYYALFAATNSSYNVAVGKDALRNCINCDNNI
                     GIGTTSLYTNQADYNIAIGYASMYANTTGDKNVTIGYNAMHDNTTGYQNTAIGYGAYE
                     NGTTGFSNTAIGFNSLASITTSYYNTAIGQDAAKNTTGFGNTAVGSLALYSNTTGSKI
                     VGIGEDSGGSEANNSYGTFIGTYAGTNGSGPYTYSTALGYSAVVDASNQVRIGIAATT
                     SIGGYTNWTNISDGRFKTNVKENVPGLDFIMALRPVTYNLDVAAINVFQHIPDSLIDK
                     NAIAQKTNQIQTGFIAQEVEKVAQQLNYDFSGVDAPQNENSTYGLRYAEFVVPLVKAV
                     QEQQSQIETLQKLVAEQNEMIKKLMQEK"
     gene            2554..3990
                     /locus_tag="PLB46_08415"
     CDS             2554..3990
                     /locus_tag="PLB46_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76579.1"
                     /translation="MSPTEKGYFRKFQAGYRVKEAKVYDYLFDLLEEMESPNDELILK
                     AMKNRCKNLQSTRAYLYQQILKSLRNYNSKKNIHFDFREQLDTIEILQSKGLHKEAYQ
                     LIEKGLLRCKATFTPTYELIFLHYQKNAIQHLEENKKPIVRNQNNTQIIAAANFITGR
                     QHIIEGYAQSIQWLNNHFPLYDEQVKSTSLDLLNQLQQIAAQHEFDFGEKNLLYAGMA
                     NICRLHGRLDEAVNYQLVTIGLMENIDLVQLNREASYVGALLNLGSMYFENKQYDSLE
                     LLIHKMTALNFHSASANMINLSVTYVLKIHLHSDTRQFEIIRKYATAGEKFFTENNSI
                     PNITFDFHIRLLAFYIHTQQYDKALSKANFMLNSSYTHSQPSFHIHVRLMNLIIHYKL
                     QHHKLLPSLFRNTYRFMSNYRHTYKTEATILNFLRRTLNSRDKFETNKVLNNLYQQLQ
                     SATSEKSEGSFFRSYFDYTEWLRSELKP"
     gene            4541..>6069
                     /locus_tag="PLB46_08420"
     CDS             4541..>6069
                     /locus_tag="PLB46_08420"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HRF76580.1"
                     /translation="MKNLLLTSCLAFTSLVGFSQTARVQVIHNSADPAAKFVDVYLDG
                     ALLLNDFKFRTATPYIDAPAGTPITISIAPSTSVSVDDAIANFTYTLTEGDKYVVVAN
                     GVLDPGAFAANPDGVSTAFNLFVRNEMREEALDAANLDFVFFHGSTDAPTVDAIARGV
                     ATVVDNAAYTAFTDYVSVPADKYIIDVTPGDVPSLIVASARAFLDGAGGASAVIFASG
                     FFDTALNPGGKSLKLFYALPDGTVQQLPLITTAKLQVIHNAADPAAAVVDIYVDGALA
                     LDNFSFRTATPYIDVPGNVPVSIAVAPPTSESVDDAIATISATFKAGYGYIAMATGVL
                     NPVLFNPNPDGASIAFNLLVKENTEFVSDRANGLEFFGVHGCTDAPTVDIIARDVATL
                     LDNVSYGAVSDYQVVPANTTYILDVALGDAPEVVVASYEASLAAVAGQSAVVYASGFL
                     SPELNPEGSLPFGLYYTLADGTTGAFPVYVMRQAAENTIATAIYPNPANANVNITLPA
                     E"
BASE COUNT         1832 a         1222 c         1027 g         1988 t
ORIGIN      
        1 atggggtgtt tatttttctt gcatgagttt ttttatcatt tcattttgtt cggctactaa
       61 tttttgtaag gtttcgattt gggattgttg ttcctggact gcttttacca gcgggacaac
      121 aaattctgca taacgaagac cgtaagtgct attttcattt tgaggcgcat ctacaccact
      181 gaaatcataa tttaattgtt gtgcaacctt ttcaacttct tgagcaataa atccggtttg
      241 aatttgattt gttttttgtg caattgcatt tttatcaatc aaagaatccg gaatatgctg
      301 aaaaacattt atcgcagcca catctaaatt ataagtaacc ggtcgcaatg ccataataaa
      361 atctaaaccc ggtacatttt cctttacatt ggttttaaat cggccatctg aaatatttgt
      421 ccaatttgta tatcctccaa tgctagttgt agctgcaatt ccaatacgaa cttgattgga
      481 tgcgtcaacc actgctgaat aacctaatgc agttgaataa gtatagggac cactgccatt
      541 agtgcctgca tatgtgccaa taaaagttcc ataactatta ttagcttctg acccacctga
      601 atcttcgcca atacccacta tttttgatcc tgttgtatta gaatacaatg ctaggctacc
      661 aactgctgta ttgccaaatc ctgttgtgtt ttttgcagca tcttgcccta ttgcagtatt
      721 gtaataagaa gttgtaattg aggctaaact attaaaacct atcgctgtat ttgaaaatcc
      781 cgtagttcca ttttcataag ctccatatcc aatagccgta ttttggtaac cagtagtgtt
      841 atcatgcata gcattatatc cgatggtgac atttttatcc cctgtggtat tagcatacat
      901 gcttgcatac ccaatggcaa tattataatc ggcctggttg gtatacaagg aagtagtacc
      961 tattccaata ttgttatcgc agttaataca atttcgtaac gcatccttac caactgcaac
     1021 attataacta ctattggttg cagcaaatag cgcataataa cctaatgcca aattataact
     1081 tgaagcggta atatttgcag cggattgagc acctaaggca gtattgcctg aaccagtagt
     1141 attatcatac aatgaataaa atccaatcgc tacattttca atgccacttg tggtagattt
     1201 taaaccacca tttccaacag ctacattata tccaccagtt actgctgatg acaatgcgcc
     1261 gcttccaatt gctgtattat tactggctgt gttgaacctt aatgcttcgt atccaattgc
     1321 tactgtctcg ctagcggtag atcccgtttc caatgcaaaa gcaccaatgg caacattttc
     1381 tgttcctgtg cccaatgatt taccactttg ataacctatc gcagtattat tgctagctgt
     1441 tgtaattgta cttagcgact tatacccaat cccggtatta gaagctccgg tagtgttatt
     1501 ttccaaagcc aacgccccta gggctgtgtt gtaatttccg gttgtattat catacattac
     1561 attataacca attcctgtat ttgaactgcc tgtattaaaa tacaagcatt tatatcctat
     1621 acctgtattt tcatttcctg caacattcga aaataatgcc tgaaaaccaa gggcagtatt
     1681 gtaatttccg gttgtgttta aatacatagc attactaccg atgccaacat tatttatact
     1741 tatatcattt cttccagcat tataacctaa aaaaaggttc tcccctgttg aaatcaaacc
     1801 cgatggcgta ttatttgttt taaaaattaa agccttatca tctgtagtgc ctaaaaaatg
     1861 agttcccggt gttgttcctg cattacctgt tgtactccat cctgtgctat tattgattaa
     1921 tggtttccaa acacttccat cgaaataata aaatgcactc tgtgtattat catacaccaa
     1981 taaaccagtt gccggcgata caattgctgt ccgttgcgca gttgtcatac gcggaattaa
     2041 tacgcctttt gttgttgatg taacatccaa catcgcactc gcatcagcta ccgtgcccgt
     2101 cgtgttgata gcaatgtttt gtgcaaacaa taaattactc attaataaac aaaaaattac
     2161 aagtgtgtag tgctgtaatc gcatattcaa agcttttgat aatttttaaa aatgaacctc
     2221 cacctgagca gaggttcgta ttcaatctgc tcacttcgct ttgaaagaat gaatttcttt
     2281 ttggggcaga tgtaatttgt cgctataatc caacaactgc tcccatgtcc gctgactttg
     2341 taacctgatt ccttttgcga agtgaacgat ttacaagggc aaagatgtgg tggtaaatga
     2401 gccaatacaa tacaaaaaag agctacttta tctattttga acaaccattt tttaaataat
     2461 attcctttat ttataggctt tttactactt ttgaccctat atttcgagtt tatggcagat
     2521 agctggaaaa cacttcatca acttattcat tctatgagcc ccaccgaaaa ggggtatttc
     2581 cggaaattcc aggcgggtta ccgcgtgaaa gaagctaaag tgtacgatta tttatttgac
     2641 ctgttggaag aaatggaatc tcctaatgat gaattaattc ttaaagcaat gaaaaatcgg
     2701 tgcaaaaatt tgcaaagcac cagagcgtat ttatatcagc aaattttaaa aagccttcga
     2761 aattacaata gcaaaaaaaa catacacttt gattttcgcg aacaattaga tacaattgaa
     2821 attttacaaa gtaaaggttt acataaagag gcctatcaat taattgaaaa agggttgtta
     2881 cgatgtaaag caacatttac tccaacttac gaattaattt ttctgcatta tcaaaaaaat
     2941 gccattcaac atttagaaga aaacaaaaaa ccaattgtcc gcaatcaaaa taatacccaa
     3001 ataattgcag cagccaattt tattaccgga cgacaacata tcatcgaagg gtatgctcaa
     3061 tccattcagt ggctgaacaa tcatttcccg ttgtatgatg aacaggttaa atcaacttcc
     3121 cttgatttac ttaatcagtt gcaacaaatt gccgcacaac atgaatttga ttttggggaa
     3181 aagaatttat tgtatgcagg tatggcgaat atttgccgac tgcatgggcg attggatgaa
     3241 gccgttaatt atcagctagt tactattggg ttaatggaaa atatcgattt ggtgcaactc
     3301 aacagagaag ccagttatgt tggtgcattg ctcaatttgg gctccatgta ttttgaaaac
     3361 aaacaatatg attcacttga attgctgata cataaaatga ctgcattaaa ttttcattca
     3421 gcctctgcaa atatgattaa tctttctgtt acgtatgtat taaaaataca tttacatagc
     3481 gacacccgac agtttgaaat tatacgcaaa tatgctactg cgggcgaaaa gttctttaca
     3541 gaaaataaca gcatcccgaa tattactttc gatttccata tccgattgct ggcattttat
     3601 attcacactc aacaatatga caaagcgtta agcaaagcca atttcatgct gaattcaagt
     3661 tatacgcaca gtcagccctc cttccatatc cacgttcgat taatgaattt aattattcac
     3721 tacaaactgc aacaccataa attattacca tctttattcc gcaacaccta tcggttcatg
     3781 tccaattacc gacataccta caaaaccgaa gccaccattc tcaatttcct tcgccgcacc
     3841 ttaaacagtc gcgacaaatt cgagactaat aaagtactta ataatttata tcaacaatta
     3901 caatccgcca cctccgaaaa atccgaaggt tcatttttta gaagttactt cgactacacc
     3961 gaatggctcc gctcagaact caagccctaa ttttatgagt aatgagtggt tcgctgcgcg
     4021 aacatgagta atgagatgtg ctaatgatac agtttctacc gtttgccaag ccattaattt
     4081 ttcttatact tatcatagat aacgctatat gcttcgtgtt actttgaggg ccttagcggc
     4141 ttcgtggctt tattcgcgaa atgaacagtt taatttgtgc attatattgg aactaaattc
     4201 atgtagtgct agcattctaa attaagaaga agaccactaa gaaaataagt gcctcaaagt
     4261 aactctaaga atttcatgcg atatactaaa taattggagt tagtagttaa aatatcatca
     4321 cttcccgcaa cttcccatct ccccgccttc ccggcaaccc ggcaacttgc atttaaatgt
     4381 taaattttaa atacaatacg cccacctgta aaacgccaca accccttacc tcacaacagt
     4441 ttgaaaattg ttcaaataat actaaaaatt aacatcacta atttttcgaa caatgcataa
     4501 tgcatttggt tttacaacca ttaaacaaac taaataagtt atgaaaaatt tactccttac
     4561 ttcgtgttta gctttcactt cattagtggg cttttcacaa actgcacgtg tgcaggtaat
     4621 tcacaactca gctgatcccg ctgcaaaatt cgtggatgtt tatttggatg gtgctttatt
     4681 attaaacgat tttaaattcc gtactgctac accgtatatc gatgcacctg ccggtactcc
     4741 tatcacaatt agtattgcac cttcaaccag tgttagcgtt gatgatgcaa ttgcgaattt
     4801 cacatacaca cttaccgaag gcgataaata tgtagttgtt gctaacggtg ttttagatcc
     4861 aggtgcattt gctgcaaatc ctgacggcgt aagcactgct tttaatttat ttgtacgtaa
     4921 cgaaatgcgc gaagaagcat tagatgcagc aaatcttgat ttcgtatttt tccatggttc
     4981 aactgatgca ccaactgttg atgccattgc acgtggcgta gcaactgttg tagataatgc
     5041 tgcatatact gcatttactg attatgtaag tgtgcctgca gataaatata ttatcgacgt
     5101 tacacctggt gatgtacctt cattaattgt tgcaagtgct cgtgcatttt tagatggtgc
     5161 aggtggcgca tctgcagtta tttttgcaag tggatttttt gatactgctt taaatcccgg
     5221 tggaaaatca ttaaaattat tttatgcatt acctgacgga acagtgcaac aattaccatt
     5281 aattaccact gctaaattac aggtaattca caatgctgct gatccagctg ctgctgttgt
     5341 agatatttat gttgatggcg ctttagccct tgataatttt tcattccgca cggctacacc
     5401 atatattgat gttccgggta atgttccggt aagtattgct gttgcacctc caacaagtga
     5461 atcggttgat gatgctattg caacaatttc tgcaacattt aaagctggtt acggttatat
     5521 cgctatggca acaggtgttt taaatccggt tttatttaat ccaaatcctg atggcgcttc
     5581 tatcgcattt aatttattag taaaagaaaa tactgaattt gtttctgacc gcgcaaatgg
     5641 tttagaattt ttcggtgtgc atggttgtac agatgctcca acagttgata ttattgcacg
     5701 tgatgttgcc acattattag acaacgtttc ttacggtgca gtatcagatt atcaggttgt
     5761 tcctgctaat actacctata ttttagatgt tgctttaggc gatgctcctg aagttgttgt
     5821 agcttcttac gaagcttcac ttgctgctgt tgctggtcaa agtgcagttg tatatgcaag
     5881 cggattttta tcacctgaac ttaatcctga aggtagttta ccattcggtt tatattacac
     5941 tttagctgat ggaacaaccg gtgccttccc tgtttatgta atgcgccaag ctgctgaaaa
     6001 tactatagca acagccatct atccaaatcc ggctaatgca aatgtaaata tcacattacc
     6061 agctgaatt
//
LOCUS       DAORKW010000182         6034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_492_length_6034_cov_5.390868, whole genome shotgun sequence.
ACCESSION   DAORKW010000182 DAORKW010000000
VERSION     DAORKW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_492_length_6034_cov_5.390868"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..532)
                     /locus_tag="PLB46_08425"
     CDS             complement(98..532)
                     /locus_tag="PLB46_08425"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRF76581.1"
                     /translation="MKYGILFLLFFYGLGKLSAQGNANAIVGVWLSENKRLKVEVYES
                     NKKYFGKILWQYEKIDPETGQPKVDKENPDPAKRNRPIIGLQVLKDFEFKDGFWQNGY
                     VYNSQNGKTYSCEIWLKGKDVLVLRGYWGLVYHTEKWTRVKE"
     gene            complement(544..972)
                     /locus_tag="PLB46_08430"
     CDS             complement(544..972)
                     /locus_tag="PLB46_08430"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRF76582.1"
                     /translation="MKRIMVLIFTLSIAFSSKAQSWQDACVGGWINGDGDAKIEIYKK
                     DNKYYGKITWLREPNEEDGKPKLDDENPDESKQSQPIMGLVILKNFDFEDGYWKNGTI
                     YDPKNGKTYKCEMWLDGKTGLKIRGYWGVVYRTETWTKAK"
     gene            1145..1717
                     /gene="nadD"
                     /locus_tag="PLB46_08435"
     CDS             1145..1717
                     /gene="nadD"
                     /locus_tag="PLB46_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRF76583.1"
                     /translation="MNIGLFFGSFNPIHTGHLIIANYFAENTDLQQVWFVVSPQNPFK
                     EKASLLDEKHRLYMVNLAIEDNYQLQASNIEFHLPQPSYTIDTLTYLKEKYPEHRFSI
                     IMGSDNLPAFHKWKNYEKILAQHPVLVYKRNNADQIPANLHGDIRLFDLPLLAISSTY
                     IRQQIKAGKSVRYLVPDKVWQHLQEMHFYK"
     gene            2150..3163
                     /gene="nadA"
                     /locus_tag="PLB46_08440"
     CDS             2150..3163
                     /gene="nadA"
                     /locus_tag="PLB46_08440"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRF76584.1"
                     /translation="MIHKGEKVVDNAILERTGFIDSPVDPSIDLFEEIRKLKKEKNAV
                     ILAHYYQDGDIQDIADYIGDSLGLAQNAAKTTADLIVFAGVHFMAETAKILNPHKKVI
                     MPDSKAGCSLADSCPPVLFKKFIEAHPGHTVISYINCTAEIKALSDIICTSSNAVKIV
                     ESVPKDQPIIFAPDKNLGRYIEKKTGRKMVLWDGSCMVHEIFSLEKITKLKHRHPQAK
                     VIAHPECEEPVLAIADFIGSTTGLLKYTINDSCNEFIVVTETGIIHQMQKDSPHKTFI
                     PAPPDNLCACNDCPHMKRNTLEKLYNCMLYELPEINIAEPTISQARKSIQRMLDISAA
                     AGL"
     gene            complement(3165..3479)
                     /locus_tag="PLB46_08445"
     CDS             complement(3165..3479)
                     /locus_tag="PLB46_08445"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF76585.1"
                     /translation="MGKPGIIVFNDVESGDLHSAGIILRAISHPLRLKILAYIDKHRP
                     VNVHSIYTNLNLDQSIASQQLNILRASNLVKTTREGKFIYYTLNYENIQRINNLIEQF
                     KK"
     gene            complement(3481..3816)
                     /locus_tag="PLB46_08450"
     CDS             complement(3481..3816)
                     /locus_tag="PLB46_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRF76586.1"
                     /translation="MPAINSIKLTSENGVEIKLEHNTLRRTVLVIRSVDHKLRQQIIK
                     MLEETPRLTVTEIYIKLRIEQSIASQHLAILRRSGVVISKRTGKFIYYSLNKERLVEI
                     GNLIKELVK"
     gene            complement(3911..4594)
                     /gene="tsaB"
                     /locus_tag="PLB46_08455"
     CDS             complement(3911..4594)
                     /gene="tsaB"
                     /locus_tag="PLB46_08455"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRF76587.1"
                     /translation="MAHILHIETSAAICSVSIAADGNLIASLNGQSINDHISQLTTLI
                     DTVVKTSGITLQQLSAIAVSAGPGSYTGLRIGVSTAKGLCHALNIPLIAVNTLTSMVE
                     GIKDTYPEKNILFCPLIDARRMEVYTLLSDHSGELLIPQQAYVMQEEHIFNSYLSCHK
                     IVFFGSGLNKCKTFLKNLNALFHEDYIQTAGSMHLLANRQYLSGKFENMAYFEPLYIK
                     EFYTTLKIK"
     gene            complement(4595..>6034)
                     /locus_tag="PLB46_08460"
     CDS             complement(4595..>6034)
                     /locus_tag="PLB46_08460"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRF76588.1"
                     /translation="KTYLQEFDAHAGKALGADNYIIVGEKLENITTIYPHPMSGNGTV
                     SGQLVKVGFGIVAPALNYDDYANIDLKGKIALIQFSSPDGTHPHSKYIDYNDERLKIK
                     TAKEKGAIAVIFYNEDENYEVPTTDYRRTTAAEDIPVMIVDKSVATQLIANKNNCTVS
                     TTLNPVIKKGHNVIGYIDNKAQNTIVIGAHYDHLGHGEIEGSLYRGAPAIHNGADDNA
                     SGVSLLIELAEQLKKSSLNNNNYLFIAFSGEEMGLFGSKAFVNSTAMQNYQINYMLNY
                     DMVGRLDSTNTIIINGVGSSTNWDVLNNIHYGDFKLKTTESGIGPSDQTSFYLKDIPV
                     LHFFTGSHNEYHKPTDDANLVNYDGIGKILSYSYVLIDSLNNEGKINFTKTKEENNDN
                     APRFTVTLGIIPDYAYSEKGMRIDGVTEGKPGSIAGILAGDIVIKMGEIEVLDMMSYM
                     QALSKFSKGQKTMVTVIRNNAEIQLEVQF"
BASE COUNT         1797 a         1137 c          976 g         2124 t
ORIGIN      
        1 caaatacggc tgtttaattg gtacattcac acatcggtac attggtacat catccggctg
       61 ttcaattagc acatcaacaa atcagcacat cagcacatta ctccttcacc ctcgtccact
      121 tttcagtatg ataaaccaac ccccaatatc cgcgcagaac caaaacatcc tttcctttca
      181 gccaaatttc acaggaatag gtttttccgt tttgggaatt atatacatag ccgttttgcc
      241 aaaacccgtc tttaaattca aaatctttta atacttgtaa tccaataatt ggtcggtttc
      301 ttttagcagg atccggattt tctttatcta cttttggctg accggtttca ggatctattt
      361 tttcgtattg ccagagaatt tttccgaaat actttttatt cgattcgtac acctcaacct
      421 ttagcctttt gttttccgat aaccatacgc ccacaatagc attagcattt ccctgagcag
      481 aaagtttccc cagcccgtaa aaaaacagta aaaaaagtat gccgtacttc atgattgcga
      541 aatttatttc gcttttgtcc aggtttctgt tctatatact actccccaat aaccgcgaat
      601 ttttaagccg gtttttccgt ccagccacat ttcgcatttg taggtttttc cgtttttagg
      661 gtcgtaaatg gttccgtttt tccagtaacc atcttcaaaa tcaaagtttt ttaaaattac
      721 caatcccata atcggctgac tctgttttga ttcatccggg ttctcgtcgt cgagtttggg
      781 tttgccgtcc tcttcattag gttcgcgcaa ccaggtaatt ttgccgtagt acttattatc
      841 ctttttgtag atttcaattt tcgcatctcc atcgccgttt atccacccgc caacacaggc
      901 atcctgccag ctttgagctt ttgatgaaaa cgctatactc agtgtaaaaa tcagtaccat
      961 tattcttttc ataaccttgt tttattccct aaaaataata aaatttttta tccattaatt
     1021 ccgccactta atacactgtc aaaattgttg ccatgttttg cgtttttact ttcttttatt
     1081 ttagcgtgac tgtttatgcg gttttttctt tttatcctct gtaatcccgt ttatctttac
     1141 aggaatgaac attggtctgt ttttcggctc atttaatcct attcataccg gacacctcat
     1201 tattgccaat tattttgcag aaaatactga tttacagcaa gtttggtttg ttgtttctcc
     1261 ccaaaatcca tttaaagaaa aggcttcatt gttagatgaa aaacatcggt tgtatatggt
     1321 taatctggcc attgaagaca attatcaact ccaggcttct aatattgaat ttcatctgcc
     1381 gcaaccaagt tacacaattg ataccctcac ttacctgaag gaaaaatatc ccgaacatcg
     1441 gttcagtatt atcatgggaa gcgataacct cccggctttt cataaatgga aaaattatga
     1501 aaaaatttta gcgcaacacc cggtgctggt atataaaagg aataacgccg accagattcc
     1561 tgctaacctg catggcgata tccgtttgtt tgatttaccc ttactcgcta tttcttctac
     1621 ttacattcgc cagcaaatta aagccggtaa atctgttcgt taccttgttc cggataaagt
     1681 ttggcagcat ttacaggaaa tgcactttta taagtaaacg tacactttat tggatatttt
     1741 taaaatattg ccttgttcca attttataat tatttggtta taagcctttt aactgttgta
     1801 aagcagatgt taataatgtg ttgtactatt gttaactcat atggataagt tgtatttttt
     1861 cttcttttaa aaagttgggt tgtctttttt tgtggagatg ttgcattgta ccaacagtgt
     1921 acgtacgttc aataaatgtg tatttcggaa aagttttcct gttaactttg taccaacagg
     1981 atgtgtatta gtcgctaaat cccatatttt attgataaaa tgctgttagt tgattgtgga
     2041 cagcattaat taaagtatgg aaaacttatt tatcaaagat gtgattaagt acttatcaac
     2101 gaatacacag cactgatgat gattctttat ttatttaaaa aataacatca tgattcataa
     2161 gggggaaaag gttgtggata atgccatttt ggagcgaaca ggatttatag atagtccggt
     2221 tgatccaagt atagatttgt tcgaagaaat tcgcaaactc aaaaaagaaa agaatgcggt
     2281 tattctagcg cattactacc aggacggaga tatacaggat attgcagatt atattggcga
     2341 tagtttggga ttagcccaaa atgcagctaa aacaactgcg gaccttattg tatttgccgg
     2401 tgtccatttt atggctgaaa cagccaaaat actgaacccc cataaaaaag tgattatgcc
     2461 cgattctaag gctggttgct cattagccga tagttgtccc ccggtattgt ttaaaaagtt
     2521 tatagaagcg catcctgggc atactgtgat aagttatatt aactgcacag ctgaaataaa
     2581 ggctttaagt gatattatct gtaccagcag taatgcggta aaaattgtag aaagtgtccc
     2641 taaagaccaa cccatcattt ttgcaccaga taaaaacctt ggtcgttata ttgagaaaaa
     2701 aacgggaaga aaaatggtgt tgtgggatgg cagctgtatg gtgcacgaaa ttttttcact
     2761 cgaaaaaata actaaactta aacatcgtca tcctcaggca aaggttattg cccatcccga
     2821 atgtgaagaa cctgttttag caattgccga ttttattgga agtactaccg gtttactcaa
     2881 atataccata aacgatagtt gtaatgagtt tatagtagtt accgaaaccg gtattattca
     2941 ccaaatgcaa aaagacagcc cgcataaaac ttttattcct gctccgcctg ataatttatg
     3001 tgcatgtaac gattgtccgc atatgaaaag aaatacgctg gagaaactgt ataattgtat
     3061 gttatatgaa ttacctgaaa ttaatattgc agaaccaacc atttctcagg caagaaaatc
     3121 aattcagcga atgttagata tttctgcagc tgcaggatta taatttattt tttaaactgc
     3181 tctattaaat tattaatacg ttgaatattt tcataattca atgtataata aatgaatttt
     3241 ccttctcttg ttgtttttac aagattagaa gctcgtaata tatttaattg ttgggaagca
     3301 atactttgat ctaaatttaa attagtataa atactgtgca cgtttaccgg acggtgttta
     3361 tcaatatagg cgagtatttt taatcgcaat ggatgtgaaa ttgcacgtag tattatacct
     3421 gcactgtgta aatcaccact ttcaacatca ttaaaaacta ttatgcctgg ttttcccatc
     3481 ttactttact agttctttta ttaaattacc tatttcaaca agacgttctt tattaaggga
     3541 gtaatatatg aatttacccg ttcgtttgct gatcactaca ccactgcgcc gtaatatggc
     3601 taagtgctgc gatgcaatgc tttgttctat acgtaattta atatagattt cagtaacggt
     3661 taaacgcgga gtttcctcca acatttttat tatctgctgg cgtagcttat ggtcaaccga
     3721 ccgaattacc aaaacggtcc ttcggagtgt gttgtgttca agttttattt ccactccgtt
     3781 ttccgaagtg agtttaatgg aatttatagc gggcatatcg ttgttttacc ggttgtcgtt
     3841 acccacgtgg cgttctgtta tcaaaactat aaaatgtaga ctgaaaaaac aaactaattc
     3901 aaacttacac tcatttaatt tttaacgtag tgtaaaattc cttgatatat aaaggttcaa
     3961 aatacgccat attttcaaat ttacctgata aatattgacg atttgctaat aaatgcatac
     4021 ttcctgcagt ttgtatataa tcttcatgaa ataatgcatt taaatttttc aaaaatgttt
     4081 tacacttatt taatcctgaa ccaaaaaaaa caattttgtg acatgataaa tacgaattaa
     4141 atatgtgttc ttcttgcatc acataggctt gttgtggaat taataattcg ccggagtggt
     4201 ctgatagcaa tgtatacacc tccatgcgtc ttgcatcaat taagggacaa aaaagtatat
     4261 ttttttcagg atatgtatct ttaattcctt ccaccatact tgttaatgtg ttaaccgcaa
     4321 taagtggaat atttaaagca tgacataaac cttttgcagt tgatacacca atacgtaaac
     4381 cggtatatga gcccggtccc gcacttactg caatagcact taattgttgt aaggtaattc
     4441 ctgaagtttt aactactgta tcaattaaag tggttaattg cgaaatatgg tcgtttattg
     4501 attggccatt taatgatgca attaaattac catctgcggc tatactcaca ctacaaatgg
     4561 cagctgatgt ttcgatatgt aatatatgtg ccaattagaa ttgtacctcc aattgtattt
     4621 cagcattatt gcgtattaca gtcaccatgg ttttttgacc tttcgaaaat ttactcagtg
     4681 cctgcatata actcatcata tctaatactt caatttcacc cattttaata acaatatcac
     4741 cggcaagtat tcccgcaata ctgcctggtt ttccttctgt tacaccatca atgcgcattc
     4801 ctttttcaga atatgcatag tcaggaataa tgcctaatgt tactgtaaac cttggagcat
     4861 tatcattatt ttcttcttta gtctttgtga aattaatttt tccttcatta tttaatgaat
     4921 caattaaaac ataactatag cttaatattt ttcctatacc atcgtaattc accaaattag
     4981 catcatcagt aggtttgtga tattcattat gtgaaccggt aaaaaagtgt aataccggaa
     5041 tatcttttaa ataaaatgaa gtttgatcac tagggccaat tcctgattca gttgttttta
     5101 atttaaaatc tccataatga atattattta aaacatccca gttagtactg ctgccaacac
     5161 cattaataat gatggtatta gtgctgtcta atcttcccac catatcataa ttcaacatgt
     5221 aatttatctg ataattttgc atcgcggttg aattgacaaa agccttactg ccaaacaatc
     5281 ccatctcttc tccactgaat gcaataaata aataattatt attgtttaga gatgattttt
     5341 tgagttgttc tgctaattca atcagtaaac ttacaccact tgcattatca tctgcaccat
     5401 tgtgaatggc aggtgctccg cgatataatg agccttcaat ttctccatga ccaaggtggt
     5461 cgtaatgtgc tccaattact attgtatttt gtgctttatt atctatataa ccaattacat
     5521 tatgcccctt tttaatcacc ggatttaatg tagtgctaac tgtgcaatta tttttattcg
     5581 ctattaattg tgtagctaca gatttgtcta caatcattac aggtatatct tcggctgcag
     5641 tagttcgtcg ataatctgta gttggaactt cataattttc atcttcatta taaaagatta
     5701 ctgcaattgc tcctttttct ttagctgttt tgattttcaa ccgttcgtcg ttgtagtcta
     5761 tatacttgct atgaggatga gtgccatccg gtgaagaaaa ttgtatcagc gcgattttac
     5821 ccttcaaatc aatattggcg taatcatcgt aatttaatgc aggagcaaca ataccaaatc
     5881 ctactttaac caattgcccg cttacagtgc cattgccact catagggtga gggtaaatgg
     5941 ttgttatatt ttctagtttt tcgcctacta ttatataatt gtcggcgcct aaagcttttc
     6001 ctgcatgggc gtcaaattcc tgcagataag tctt
//
LOCUS       DAORKW010000183         6018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_498_length_6018_cov_5.604897, whole genome shotgun sequence.
ACCESSION   DAORKW010000183 DAORKW010000000
VERSION     DAORKW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_498_length_6018_cov_5.604897"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLB46_08465"
     CDS             <1..918
                     /locus_tag="PLB46_08465"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF76589.1"
                     /translation="GTSHNGLYKYDGKSFKQFLVTNGLNSNNIACLLEDKEGKIWIGT
                     DAGLCLYDGKSFTQIQIPLPADLPPNKNQNFINSHWVCSIMQAKNGDLWLVTIDGVYV
                     YDGQTYKLFIINEAENSFVGSNDKIERILEDKTGNIWFGGRTNEGVFRYNGKSITHFK
                     LPEITLQFESKKVIHSWGWPQVQDKNGDIWFSNWAGAYRYDGNTFTSYSTNDGLAGYN
                     GIVSKIIEDKSGNLYFGGDGGLSRYEPTLRNEVEKNKSFTCFKDGLINPWIWMILEDN
                     TGSIWVGTRETGLYLFDGKSFINYSEDKQ"
     gene            1065..1487
                     /locus_tag="PLB46_08470"
     CDS             1065..1487
                     /locus_tag="PLB46_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HRF76590.1"
                     /translation="MNGFKITTPDNQEIKFQFYLDEAPVTSKAFVDILPFTRTFHHAR
                     VSGQEIWIDNVPQLDIIQENASVFTEPGEVVFGPTKPSRTKTANCFGIYYGEGKGLDA
                     CNIFARIIESDRQKLIDLGNNIWKNGSQDLTIAAIDII"
     gene            1519..1965
                     /locus_tag="PLB46_08475"
     CDS             1519..1965
                     /locus_tag="PLB46_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF76591.1"
                     /translation="MIIREAKIPDIKQIQIVRNAVKENVLSNPGLVTDQDCEEFITIR
                     GKGWVCEIDKQIVGFAIADLKENNIWALFLDPEFEGKGIGQLLHNTMLDWYFSQTKTT
                     VWLGTGFNTRAEKFYRIAGWTEVGTHGPKEIKFEMTYDDWTKIRNR"
     gene            2126..2341
                     /locus_tag="PLB46_08480"
     CDS             2126..2341
                     /locus_tag="PLB46_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76592.1"
                     /translation="MAISKAILPNTTSDLFLTDGGLETTLVFLEGYELPCFAAFDLLK
                     DENGILAIKNYYRRYLSIAKNLNQGSF"
     gene            2383..3075
                     /locus_tag="PLB46_08485"
     CDS             2383..3075
                     /locus_tag="PLB46_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRF76593.1"
                     /translation="MGYSASSITDVNEKAVQLLVDLKHEFQNDVPNIILSGCVGPRGD
                     GYIPENTMTAIEAQQYHSAQIAVFSQTSVDMVSAITMNYAEEAIGIACAAAEVNLPVV
                     ISFTLETNGKLPTGMSLKEAIELVDKSASKPPIYYMINCAHPTHFLHELQTGQHEVWI
                     NRIKGLRANASCKSHAELDAATELDRGNPLELGSEHKELKEMLPQLNVFGGCCGTDEE
                     HVLAIASQVITN"
     gene            3313..3792
                     /locus_tag="PLB46_08490"
     CDS             3313..3792
                     /locus_tag="PLB46_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRF76594.1"
                     /translation="MLYFLFVLLFFAVLVLITRPFTVIFHELGHAIPAILMTRQKVSI
                     YIGSHGNPKKSLHFGIGLLKVLFKYNPFLWERGLCVPSSKQISINKQIIYTFTGPLAS
                     IVIAAIVSSFAFSNDIHDFLRIFLIIFISSAIYDLFVNLTPSDTAVQLYNGDNTYNN"
     gene            3827..5632
                     /locus_tag="PLB46_08495"
     CDS             3827..5632
                     /locus_tag="PLB46_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF76595.1"
                     /translation="MKYLIATLYIFLVLMTACNGQTNYSQQTQEKIKEVENNITGNLI
                     LNDEAPSTIIERMQKYNVKGVSIAVIENYKVVWAKGYGWADEEDKRPVTTETLFEPGS
                     ISKSLNAVGILKLAQDQQVDLYTDINTYLTSWKFPYDSLSNGKKITLADILSHNAGLS
                     VHGFPGHDINDPFPTVIDVLDGKKPAVTDPVRSMFEPGVMFQYSGGGTTISQLILTDV
                     TKQAYDTWMYENILKPIGMVNSSFSQPPSKKNQPFCATGYLSDGTPISNKYHVYPELA
                     AAGLWMTPTDLCNYIIDMQLAYQGKKKSKVLNDEMVKKHLTPYNNGPTSMGSFIVDLD
                     GAKYFEHGAGNDGFSGDFYGSLEDGYGCVVFINSEEHRLIAEIINSIAKAYNWKNFYR
                     EPKKKTSIAVSDDVIKPAEGIYLFDQSWAAVGKRNNEYHFYTSGIYAKMYFSSPNTFF
                     NEEFQAVKTFITDSTGHITGYKRNVDGKEYPNAIKITKPETLKSESMVFHEISLYLFE
                     NKRYAESAVYLQRAVELFPEDLELLLNLANMYLYNNEYDKAIAIHEAHLNDNVGNNYT
                     WTSLLHFYVDYYRDKKIDVQVFEKVYKELNLEWGN"
BASE COUNT         2039 a          989 c         1191 g         1799 t
ORIGIN      
        1 ggaacctctc ataatgggct gtataaatat gatgggaaat cgtttaagca atttttagtg
       61 accaatggac ttaacagcaa taatattgct tgtttgttgg aggataaaga aggtaaaatt
      121 tggattggta ctgacgcagg actttgttta tacgatggaa aatcttttac ccaaatccag
      181 attccacttc cagcagatct gcctcccaat aagaaccaaa attttatcaa ctcgcattgg
      241 gtgtgcagta ttatgcaagc taaaaacggc gatttgtggt tggtaactat tgatggtgtt
      301 tatgtttacg acggccaaac ttataagcta tttattatca atgaggctga aaatagtttt
      361 gtaggcagta acgataagat agagcgtatt ttagaagata aaacaggaaa catttggttt
      421 ggtggacgca ccaacgaagg tgtttttcgc tacaacggta aatcaataac tcactttaaa
      481 ctaccggaga taacgcttca atttgagtcg aaaaaagtta ttcatagttg gggatggcct
      541 caggtgcaag ataaaaacgg agatatatgg tttagtaatt gggctggcgc ctatcgttat
      601 gatggaaata catttacaag ttattcaact aacgatgggt tagcaggtta taatggtatt
      661 gtgtcgaaaa ttatagaaga caaaagcggc aacctttatt ttggtggtga tggtggactt
      721 tctcgttatg aacctaccct gagaaatgaa gtcgaaaaga ataaatcatt cacatgtttt
      781 aaggatggat taatcaatcc ttggatatgg atgattttag aagataatac cggaagtatt
      841 tgggtcggta ctagagaaac cggactctac ctcttcgatg ggaaatcatt tattaattat
      901 tcggaagata aacaatagcg aattccatat tccgaagatg gtttttcaga tatataaagt
      961 tagtaactac gattgtacat tgatttaaat aaattacaca ttgtaaattt cgaaatatac
     1021 ttgctcattt ttaattctaa acaataaatc attcaaataa tagtatgaac ggttttaaaa
     1081 taacaacgcc ggacaaccaa gaaataaaat ttcaatttta tcttgacgag gcacctgtca
     1141 cgtcaaaagc ttttgtcgat atattaccat ttacacggac ttttcatcac gcaagggtat
     1201 ccggacaaga gatttggata gacaatgttc cccaactgga tattattcag gagaatgcat
     1261 ctgtttttac cgaacctggt gaagttgttt ttggaccaac aaaaccttcc cggacaaaaa
     1321 cagccaactg ttttggaatt tactacggag aaggtaaagg acttgacgcg tgtaatattt
     1381 tcgcaaggat aattgaaagc gaccgacaaa aattgattga cctcggaaat aatatttgga
     1441 aaaacggctc acaggatttg acaattgctg ctattgatat tatttaacaa cgaacaataa
     1501 aactacagaa gataatttat gattattaga gaagctaaaa taccagacat aaaacaaatt
     1561 caaattgtaa ggaatgcggt aaaggaaaat gtattgtcaa atcctgggtt agtgacagac
     1621 caagattgtg aagaatttat tacgataaga ggtaaaggct gggtttgtga aattgataaa
     1681 caaattgtgg ggtttgcaat tgcggacttg aaggaaaata atatttgggc tttatttttg
     1741 gaccctgaat ttgaaggaaa aggcatcggg cagttactac ataataccat gttggattgg
     1801 tatttttcac agacaaaaac tacagtttgg cttggaaccg ggttcaacac cagagctgaa
     1861 aaattttaca gaatagccgg ttggacagaa gtcgggacgc atggtcctaa agaaattaag
     1921 tttgaaatga cgtatgatga ctggacaaaa atcaggaatc gctaatgcgg atatttattt
     1981 gtgtgatatt cctgaaaggt atctcttttc aagggctttc cataatttag gttgggactt
     2041 ctattcgact tttgtaaata agctttaagt ttcggcttca ataggtcaca agtccaaaca
     2101 aacaggcact actaaaatta tcattatggc aataagtaaa gcaattttac caaatactac
     2161 ctccgattta tttttaactg atggcggcct ggaaaccacc ttggtttttc tcgaaggtta
     2221 tgaacttcca tgttttgcag cttttgattt attaaaggat gaaaatggga ttcttgcaat
     2281 aaagaattac tatagacggt atttgagtat tgcgaaaaat ttaaatcagg gttcatttta
     2341 gaaagtccaa catggcgggc aaatcctgat tggataagaa aaattggcta ttcagcttca
     2401 tcaattacag atgtgaatga aaaagcagtt cagctacttg ttgacctcaa acatgaattt
     2461 caaaacgatg ttcccaatat tattttaagc ggatgtgtcg ggcctcgtgg agatggttat
     2521 attcctgaaa atacaatgac tgcaatagaa gcacaacaat atcattcagc gcaaattgct
     2581 gtgttcagcc aaacttcggt tgacatggtt tcggctataa ccatgaatta tgctgaagaa
     2641 gcaattggaa ttgcctgtgc agcagctgaa gttaatcttc cggttgtaat ttcatttact
     2701 ttagaaacta atggtaaact tccaaccggt atgagtttga aagaagcaat tgagttggtt
     2761 gataaaagtg catcaaagcc tccaatttat tatatgatta attgcgcgca cccaacacat
     2821 ttcttacatg aattgcaaac aggacagcat gaagtttgga ttaacagaat aaaaggacta
     2881 agagcaaatg cttcttgtaa aagtcatgct gaattggatg cagcaacaga attggataga
     2941 ggtaatccac ttgagctggg tagtgaacat aaagaactta aagaaatgtt gccacagcta
     3001 aacgtatttg gtggatgctg tggaaccgat gaagaacatg ttcttgcgat tgctagccag
     3061 gtaataacca attaatggtt gttaacttgt tttttcccat aacaatgaag ttgtatgttt
     3121 taatgttggg cggttcatcc aatattagat tattaaatca acaataatgt tgaaatgttt
     3181 tgatgcaaga aatatacaag ccgcaacaaa taaccaaata ggctttacca atttcttaac
     3241 tgcaatcata tcaaaaagca acaatatgga aaatgcaata ctaaaaccac ataaacataa
     3301 actgggcaac taatgctgta tttccttttt gtacttttgt ttttcgccgt gcttgtactt
     3361 attacaagac catttactgt gattttccat gagcttgggc atgcaattcc ggcaatttta
     3421 atgacaagac aaaaagtttc aatttatatt ggttcacatg ggaacccgaa aaagagcttg
     3481 cattttggaa ttggactatt aaaagttttg tttaagtata atccattttt gtgggaacgt
     3541 ggactttgtg taccttcatc taaacaaatt tctatcaaca aacaaatcat ttatactttt
     3601 acagggccat tagcttctat tgtaatagca gcaatagtta gcagtttcgc tttttcaaat
     3661 gatatacacg attttctaag aatctttttg attatattta ttagttcggc aatctatgat
     3721 ttatttgtaa accttacacc aagtgacacg gcagtgcaac tttataatgg tgataataca
     3781 tacaataact aaccttgaca acagtaaaaa tataaatact caactaatga aatacttaat
     3841 agcaacattg tatatatttc tggtgctaat gactgcctgc aatggtcaga ctaattactc
     3901 acaacaaaca caagaaaaaa tcaaagaagt tgagaataat attacaggca atctcatctt
     3961 aaatgacgag gcgccgagca cgattataga acgcatgcaa aaatataatg ttaagggagt
     4021 gagtattgcc gttatagaaa attataaagt ggtttgggct aaaggatatg gttgggccga
     4081 tgaagaggat aaaagacctg tcaccacaga aacgttattt gaacctggct ctatcagcaa
     4141 atctttaaat gcagtgggta ttttaaaatt agcacaggat cagcaagtgg acctttatac
     4201 agacatcaac acatacctga cttcctggaa atttccatac gattcacttt cgaatggtaa
     4261 aaaaattaca ttggctgata ttttaagtca taacgcagga ttatctgtgc atggatttcc
     4321 cgggcatgat atcaatgacc catttccgac cgttattgat gttttagatg gcaaaaaacc
     4381 tgctgttacg gatcctgttc gcagtatgtt cgagccggga gttatgtttc aatattcagg
     4441 agggggaact accatttcgc aattaatcct tacggatgtt accaaacagg cttatgatac
     4501 ctggatgtat gaaaacattt taaaacccat tggaatggtg aatagctcat ttagtcagcc
     4561 accatctaaa aaaaatcaac cattttgtgc aacagggtat ttgtctgatg gcacaccaat
     4621 ttccaataaa tatcatgttt atcctgaatt agctgctgcc ggactgtgga tgacccctac
     4681 agatctttgt aattacatta ttgatatgca attggcttat cagggcaaaa aaaaatcaaa
     4741 agtattgaat gatgaaatgg ttaaaaaaca tttaacgccg tataacaatg gaccaacgtc
     4801 tatgggttca tttatagtag accttgatgg ggctaaatat tttgagcacg gtgcaggtaa
     4861 cgatggattt agtggtgatt tttatggcag tctggaagat ggttatggct gtgttgtttt
     4921 tataaattca gaggagcata gattaatagc agaaattata aacagcattg caaaagccta
     4981 caactggaaa aatttctata gagaacctaa aaagaaaacc agtatcgctg tctcagatga
     5041 tgtaataaaa ccggcagaag gaatttacct attcgatcag agctgggcag ctgtaggtaa
     5101 aaggaataat gaatatcatt tctatacaag tggtatatat gcgaaaatgt attttagttc
     5161 acccaataca ttttttaatg aagaattcca ggcagtcaag acctttataa cagactcaac
     5221 agggcatatc accggttaca aaagaaatgt ggatggtaaa gaatatccaa atgcaataaa
     5281 aatcactaaa cccgaaacgc taaaatcgga gagtatggtg tttcatgaaa ttagtttgta
     5341 tttattcgaa aataagcgat atgctgaatc tgcggtgtat ttacaacgag ccgtagaact
     5401 ctttcctgag gaccttgaat tattattaaa ccttgcaaat atgtatttgt ataataacga
     5461 atatgataaa gcaatagcaa tacatgaagc gcatttaaac gacaatgtgg gaaacaatta
     5521 tacttggacc agtctgttac atttttatgt agactattat cgtgataaga aaattgatgt
     5581 tcaagtattt gaaaaggtat ataaagaatt aaatctggaa tggggaaatt aattgttttc
     5641 aacgtcctct attttatgca agcgcgctaa gttgtaaaac caggttcacg cggagaaaaa
     5701 ggaaaaaatc ggagacgata accggctgtt taatcagcat ccgccgcggc ggacgcatag
     5761 cgcattggta catcaacaca ttggtacatt attcggctgt taatcagcac atcagcacat
     5821 caccacatta gcacatcatt ggctgtttcc gcctcagcgg acggttcgaa ttggtatcca
     5881 ctaacgcgga cgcttcgcgc atcggtacat tggtacattc gcacattatt cggctgttaa
     5941 tcagcacatc aacacatcag cacatcacca catcattggc tgtttccgcc tcagcggacg
     6001 gttcgaattg gtacatcg
//
LOCUS       DAORKW010000184         6015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_500_length_6015_cov_4.902181, whole genome shotgun sequence.
ACCESSION   DAORKW010000184 DAORKW010000000
VERSION     DAORKW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_500_length_6015_cov_4.902181"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLB46_08500"
     CDS             complement(<1..695)
                     /locus_tag="PLB46_08500"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRF76596.1"
                     /translation="MKFLLEMIKFKTLFVVASLFILTPITLVAQSDKDLLKRPSPPRL
                     VNDVANVIDDAAEAQLENKLVHYNDTTSTQIAVITISSVEPFEIGDYAYKIGRYWGVG
                     NANFDNGIVLLVAVDDHRMFIATGYGTEAYLTDIRCNQIIDQILTPAFQQNDFYGGID
                     NATTEMMKYMTGAYEGQPYGNANDAPPTLIIIIIIIIIIFFISRRTKGGGGTTFSRRG
                     PTYWGGGGIGGLGG"
     gene            complement(670..1113)
                     /locus_tag="PLB46_08505"
     CDS             complement(670..1113)
                     /locus_tag="PLB46_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRF76597.1"
                     /translation="MPQARTFFTAEQQQEIIRAIRNAEMDTSGEIRLHLEESCGDDVL
                     DRAADIFRKLEMHKTELRNGVLIYLAVKDRQFAIIGDVGINEKVPENFWDDIKEDMVA
                     NFKQGDFVKGLINAIETSGKELQHFFPRNAGDINEMSDEISFRND"
     gene            complement(1117..1743)
                     /locus_tag="PLB46_08510"
     CDS             complement(1117..1743)
                     /locus_tag="PLB46_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRF76598.1"
                     /translation="MATTTPAAPAKSRTGLFITLGVILVLILIIRSSFNGMVAADETV
                     NEKWNNVESAYQRRLDLIDNLVNTVKGAADFEKSTLTEIIEARSKATNVTIDPSKATP
                     EQLQSFTEAQNGVNSALSRLLVVVENYPTLTATQAYRDLMSSLEGTENRINVARVDYN
                     EAIKNYNTRIRKFPANILASITGFEKRNGFTAAEGADKAPKVDFSDNK"
     gene            1889..2230
                     /locus_tag="PLB46_08515"
     CDS             1889..2230
                     /locus_tag="PLB46_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRF76599.1"
                     /translation="MSELVNEFNDYRSKMNEVILGKQNKVINRLFNLDTNTYAEGALS
                     VKTKEMLGLVASMVLRCDDCIKYHLGKCFEEGVTTEEIYEIFAVANIVGGTIVIPHTR
                     RGAEYWEALIA"
     gene            2345..3211
                     /locus_tag="PLB46_08520"
     CDS             2345..3211
                     /locus_tag="PLB46_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76600.1"
                     /translation="MNYLLLVLITSLSLGSGRELKHYNFEQPDKIISLPDSLREISGI
                     AFINDSTVVCVQDENGILFVYDIQHNRIKERHPFHADGDYEGITTAYNYIYILRSDGK
                     LFEISDFNSKKISTKSYTTNVPASNNEGLCFNYGENELLIGCKNKVTNSDVEKDVRQI
                     YAFDLSEHKLDKNAKFEMRVEDAMTFADKKNLDMSGVGANKKGKNKNTFKFNISAIAI
                     HPISGNLYILNAADHALFVVNDKSKIIAIQLLDPVLFNKPEGLTFKPNGDMFITNEGQ
                     EGNANIVSLTYH"
     gene            3318..4832
                     /gene="lysS"
                     /locus_tag="PLB46_08525"
     CDS             3318..4832
                     /gene="lysS"
                     /locus_tag="PLB46_08525"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRF76601.1"
                     /translation="MSQQFSEQEQIRRESLLALRNMGIEPYPAASFEISHSAATILKQ
                     FPEQPDSFKHVSLAGRVMGKRIMGKASFAVIQDSTSKIQIYVKGDEICPGEDKSLYEE
                     VFKKHLDIGDIIGVKGYVFTTKTGETSIHVSEFSLLSKSLRPLPIVKTDAEGNVHDAF
                     TDSEQRYRMRYVDLIVNPDVREVFIKRTKLVNTIRTFLNSFGYIEVETPILQPLYGGA
                     AARPFKTHHNALDMTLYMRIANELYLKRLIVGGYDGVYEFSKDFRNEGMDRFHNPEFT
                     QVEFYAAYKDYIWMMDFVEEMIEKVAIEVNGTTEVQVGNNLISFKRPWKRFTMFEAIE
                     HFTGIDISNMDENEIYKTAKSLKVHVDATMGKGKLIDEIFGEKCEPFLIQPTFITDYP
                     VEMSPLAKKHRSKEGLVERFEAICNGKEMLNSFSELNDPIDQRHRFESQLELGKRGDE
                     EAMQLDEDFLRALEYGMPPTAGIGIGIDRLTMIMTNQHAIQDVLFFPHMRPEAK"
     gene            complement(4866..>6015)
                     /locus_tag="PLB46_08530"
     CDS             complement(4866..>6015)
                     /locus_tag="PLB46_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76602.1"
                     /translation="IAFVGYNADNNDQFTIITTNYLEDGIQIYFVDAGWTGSAIYASE
                     GGTLWTVPSGGVPTNTMVTFTGGGAGGFTTFPSGNLEAGVTVSSGTIAASGIAASMAF
                     STNGDQILAFTGSVASPTFIAAVNFNGAWSWTGTNNAEQTLLPPGLTDGTNCVLLNDR
                     DNGKIDCLLLPDPASVSDYNNAAYWVYNDATRYTLPPTLGTCEFLLPLDLIAFYGTAN
                     TKGNTLTWSTAHEMLLSGFEVQRSINGNDFIHLSFIPAAGTSNNVITYQFNDDVTTAS
                     QYYYRLKMIDDDDSFSYSEIITISQTQKDAAYVFPNPAGEIVTIVIPENMSIQTLQMF
                     ASNGNLIREVQFETTNNYLQFDVSDLANGMYILRLNSASQYQSLSFMK"
BASE COUNT         1901 a         1089 c         1138 g         1887 t
ORIGIN      
        1 cctcctaagc cgccgatgcc tccaccaccc caataggttg gtcctcttct gctaaatgtt
       61 gttcctccac caccttttgt tctgcgcgag ataaaaaaga tgatgataat aatgatgatg
      121 ataataatta aggttggtgg tgcatcgtta gcattgccat aaggttggcc ttcgtatgca
      181 ccggtcatat atttcatcat ttcagtggtt gcattgtcga taccaccata aaaatcgttt
      241 tgctgaaatg cgggggtgag aatttgatca ataatttgat tgcaacgaat atcagttaaa
      301 tacgcttcag taccgtaacc ggttgcaata aacatgcggt ggtcgtcaac cgcaactaat
      361 aatacgatac cattatcaaa atttgcatta ccaactcccc aataccgacc aattttataa
      421 gcgtaatcgc ctatttcaaa aggttcaaca ctagatatgg taattactgc aatttgtgta
      481 gaagtggtgt cgttataatg tactaattta ttttcaagtt gagcttctgc tgcatcgtca
      541 attacattgg caacatcatt taccaaacgc ggtggtgacg ggcgtttcag caagtcttta
      601 tcgctttggg caacaagtgt aatcggcgtt aaaataaaca gcgaagcaac aacaaatagt
      661 gttttgaatt taatcatttc taaaagaaat ttcatcgctc atttcattaa tatcgccagc
      721 gttgcgaggg aaaaaatgtt gaagttcttt acctgaagtt tcaatggcat tgataagccc
      781 tttcacaaaa tcgccttgtt taaaattggc taccatatct tcttttatat catcccagaa
      841 attctccggt actttttcat ttattcccac atcaccaatt atcgcaaatt gtctgtcttt
      901 tacggcaaga taaattagta cgccattgcg caattccgtt ttatgcattt cgagtttgcg
      961 aaaaatatct gcagcacgat ctaatacatc atcaccacaa gattcttcca ggtgtaaacg
     1021 aatttcacct gaggtatcca tttcagcgtt gcggatggca cgaattatct cctgctgttg
     1081 ttcagcagta aaaaaggttc ttgcttgagg catctgttat ttattatcgc taaagtcaac
     1141 cttaggcgct ttatctgcac cttcagcagc agtaaatcca ttgcgttttt cgaagccggt
     1201 tatactcgct aaaatattgg caggaaattt cctgatgcgg gtattatagt ttttaatagc
     1261 ctcgttataa tccacacggg ctacgttaat tctattttca gtgccttcta acgaactcat
     1321 caaatcgcga taagcctgtg ttgcagttaa tgttgggtaa ttttcaacta ccaccaataa
     1381 tctgcttaat gctgaattta ctccattttg agcttccgta aatgactgta attgctccgg
     1441 tgtagctttt gaaggatcga tagtaacatt ggtagctttt gaacgagctt cgataatttc
     1501 agtaagggtc gatttttcga aatcggcagc accttttacg gtatttacca aattgtcgat
     1561 aaggtcgaga cgacgctgat aggcagattc cacgttattc catttttcat ttactgtttc
     1621 atcagctgct accataccat taaacgagct tctgattatc aaaataagca ccaggattac
     1681 acctaaggtg ataaatagtc cggttctaga ttttgccgga gctgcgggag ttgtagttgc
     1741 cattgtttgt taaattgtgt ttgtaaagat aaacctaata gttaaataat ataacaatag
     1801 gacgacctga ccgttcaaat attgtataat ctcaatcaaa atttgcagtt tcctccatca
     1861 aatatgaact ttgaacaaaa tacctgttat gagcgaatta gtaaatgaat ttaacgacta
     1921 ccgttcaaaa atgaacgaag taatattggg caaacaaaat aaagtgatta accgtttgtt
     1981 taacctcgac accaatacat atgctgaggg cgcactttca gttaaaacca aagaaatgtt
     2041 aggtttggtt gcgagtatgg tgcttcgttg tgatgattgt attaaatacc acctgggtaa
     2101 atgttttgaa gaaggtgtta ctactgaaga aatttatgaa atttttgctg tggccaatat
     2161 tgttggtgga accattgtaa ttcctcatac cagaagaggt gcggaatatt gggaagcttt
     2221 aattgcttaa ttcaattatt gtttttattt agcattccat ttattgttaa attaattgca
     2281 ataattatta atacttctcc caacaaaaat gatttctagt tgaaaaatga ttattttcgt
     2341 aaggatgaat tatttactgt tagttttaat cacatcactt tccttgggtt ccggcagaga
     2401 gcttaaacat tacaattttg agcaacctga caaaatcatt tccctaccgg attcattacg
     2461 agaaatatcg gggatagcat ttattaacga cagcaccgta gtttgcgtgc aggatgaaaa
     2521 tggaatttta tttgtttacg atattcagca taaccgaata aaggagcgac atccatttca
     2581 tgcagatggc gattatgaag gtattactac tgcatacaat tacatttata ttttacgcag
     2641 cgatggcaaa ttatttgaga taagcgattt taacagcaaa aaaatatcta ccaaaagtta
     2701 tactaccaat gtgcctgctt caaataatga gggcctgtgt tttaattatg gggagaatga
     2761 attattgata ggttgtaaaa ataaagtcac caattcagat gtcgaaaaag atgtacgtca
     2821 gatatacgca tttgatttat cggaacacaa attggataaa aatgccaaat tcgaaatgcg
     2881 ggtagaggat gcaatgactt ttgccgataa aaagaattta gacatgagtg gtgttggggc
     2941 aaacaaaaaa gggaaaaata aaaatacatt taaatttaat atttcagcca tcgctattca
     3001 tcccatttcg ggtaatttat acattttaaa tgctgctgac catgcgttgt ttgttgtgaa
     3061 cgacaaaagc aaaataattg ccattcaact tttagaccca gtgttattta acaaacccga
     3121 aggtttaacc tttaaaccaa atggcgatat gtttatcacc aacgaaggcc aggaaggcaa
     3181 cgcaaatatt gtatcactta cctatcactg atagttcttg agactgaaat tgaagccagt
     3241 catttcaaaa aaaaggataa attccctatc tccggttgta tctgcgtatc tttgccgcaa
     3301 attatcaggt taatacaatg tcacaacaat tttcagaaca agaacaaatt cgacgcgaaa
     3361 gccttttggc attacgcaac atgggtattg aaccctatcc tgctgcatca tttgaaatta
     3421 gtcattctgc tgctaccatt cttaaacagt ttcccgaaca accggattcg ttcaagcatg
     3481 ttagtttagc cggaagggta atgggtaaac gtattatggg aaaggcgtcg tttgctgtta
     3541 ttcaggatag tacatctaaa attcagattt acgtgaaggg agatgaaatt tgtccgggtg
     3601 aagacaaatc gttgtatgaa gaagtattca aaaaacattt agatataggt gatataattg
     3661 gtgttaaagg atatgtattt actacaaaaa caggtgaaac atctatccac gtgagtgaat
     3721 tcagtttact cagcaaaagt ttacgtccgc tgccaatagt taaaaccgat gccgaaggaa
     3781 atgtgcacga tgcatttacc gatagcgaac aacgttacag aatgcgttat gtggatttaa
     3841 ttgttaatcc tgacgtgcgt gaagtattta ttaaaagaac taaactggta aataccatcc
     3901 gtaccttttt aaatagtttc ggatatatag aagttgaaac cccaattcta caaccacttt
     3961 atggtggtgc tgctgcacgt ccgtttaaaa cgcatcacaa tgcattggat atgacattat
     4021 atatgcgaat tgcgaatgaa ttgtatttaa aacgattgat tgttggcgga tatgatggag
     4081 tatatgaatt ttcaaaagat ttccgcaacg aaggtatgga caggttccac aatccggaat
     4141 tcactcaggt ggaattttat gctgcttata aagattatat ctggatgatg gattttgtgg
     4201 aagaaatgat agaaaaagtt gctattgaag taaatggcac aaccgaagtt caggtgggca
     4261 ataatcttat ttcctttaaa cgtccttgga aacgatttac catgtttgaa gccatagaac
     4321 attttacagg cattgatatt tcgaatatgg atgaaaatga aatttacaaa actgcaaaat
     4381 cactcaaagt gcatgtagat gctacaatgg gtaaaggcaa actgatagat gaaattttcg
     4441 gtgaaaaatg tgagccgttt ttaattcagc caacatttat aaccgattat cctgttgaaa
     4501 tgagtccgct tgctaaaaaa catcgttcta aagaaggttt agttgaacgt tttgaagcta
     4561 tttgtaatgg caaagaaatg ttgaattcat ttagcgaatt aaacgaccca atcgaccagc
     4621 gtcatcgctt cgaatcgcaa ttggagttag gcaaacgcgg cgatgaagaa gctatgcaat
     4681 tagacgaaga ctttttacgc gcactcgaat atggtatgcc accaacagca ggcatcggaa
     4741 ttggtattga tcgtttaacg atgattatga ccaatcaaca tgccattcag gatgtgttgt
     4801 ttttcccaca catgcgcccg gaggctaagt aatattattg ttataacttt tgtttatcgt
     4861 ttatgttatt tcataaacga taaactttgg tattggcttg cactatttaa acgcaatata
     4921 tacatgccgt ttgctaaatc ggagacatca aattgtaaat aattgttggt tgtttcaaat
     4981 tgcacttcac gaatcaaatt accatttgaa gcaaacattt gaagtgtttg aatggacata
     5041 ttttccggta ttacaattgt gacaatttct ccggcaggat tcgggaatac ataagcagca
     5101 tctttttgtg tttggctgat ggtaattatt tcgctatatg aaaaactatc atcatcatct
     5161 atcattttta atcgataata atattgtgaa gctgttgtta catcgtcatt aaactgataa
     5221 gtaatgacat tgttgcttgt tcctgctgcg ggaataaaac tgagatgaat aaaatcatta
     5281 ccatttatac ttcgttgaac ttcaaaacca ctcaataaca tttcgtgagc agttgaccat
     5341 gttaatgtat ttcctttggt attagcagtg ccataaaatg caattaaatc cagtggcagc
     5401 aaaaattcac atgtgcccag tgttggcggt aatgtatatc gcgtagcatc attatatacc
     5461 cagtatgccg cattattata atcactaact gaggcagggt cgggtaataa taagcagtct
     5521 attttaccat tatctctatc gtttaataat acacaattgg tgccgtctgt taatcccgga
     5581 ggtaataatg tttgctctgc attatttgtg cctgtccatg accaagcacc attaaaatta
     5641 acagcagcaa taaacgtagg gctggcaact gagccggtaa atgccaaaat ctggtcccca
     5701 tttgtagaaa aagccatact ggcagcaatt ccgcttgctg caattgttcc tgatgaaact
     5761 gttacaccgg cttctaaatt tcctgatgga aaagtggtaa atccacctgc accacctccg
     5821 gtaaatgtaa ccatcgtatt ggtaggcaca ccacctgaag gaacagtcca cagagttcct
     5881 ccttcactgg cataaattgc cgaacctgtc catccagcat caacaaaata aatttgaatg
     5941 ccatcttcca aataattggt tgttataatg gtaaactgat cgttattatc agcattgtag
     6001 cctacaaaag caatg
//
LOCUS       DAORKW010000185         5989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_502_length_5989_cov_3.931244, whole genome shotgun sequence.
ACCESSION   DAORKW010000185 DAORKW010000000
VERSION     DAORKW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_502_length_5989_cov_3.931244"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLB46_08535"
     CDS             <1..719
                     /locus_tag="PLB46_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HRF76603.1"
                     /translation="DAVCSPATNQWLKQVVSGYATENKAIVLGYGAYEKKPGRLNRRI
                     RYETFLTALNYLSFALAKLPYMGVGRNLSYQKGLFFSTNGFFDHRHIPSGDDDLFINK
                     VATKRNTEIVISPDAFTYSPPKETREEWIEQKTRHMSTARYYRAKHKFWLALQPISHI
                     LFWITSLYLLATVFVYWYIVAAAFVLRWALLRYTFGQSMNKLDEKDLKPYIELYDIMQ
                     IGYYFRFAKAALVKTKYRWD"
     gene            710..1312
                     /locus_tag="PLB46_08540"
     CDS             710..1312
                     /locus_tag="PLB46_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF76604.1"
                     /translation="MGLNENLSDRAREDMELVEKARKGDQYAYSVLMARYKDSINFML
                     LKMVHNRDDADDLTIEAFGKAFLHLEKYTPDFAFSTWLFKIALNNAIDFIRKKKLKTL
                     SLDDENDESSKYVFSKIKTSALDPEESIIKDQRAGIMRTILDELNPKYKTLIEMRYFE
                     ELSYEEIAEKLNMPLGTVKAQLFRARNFLYELLAERKGRI"
     gene            complement(1465..2814)
                     /locus_tag="PLB46_08545"
     CDS             complement(1465..2814)
                     /locus_tag="PLB46_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76605.1"
                     /translation="MLKHLLFVLCFGAFSIGIGQTYFEGGIFTPTNWTLAESPYIITG
                     NVTVFPGGSLNINPGVEVKVNDGFIIEIRDNKFTAIGTEGQPIIIKSNNPTPSQTSWE
                     GILLTDSSSLEFEYVNISDAHKAIDYELPPTPISASDVITCKNCNFQNNYYGITGTTN
                     FPKSYFFQCTFDNNQTGVSIDDQEGYPAVFEGCNFTNNAGGALTNYSTFINTDFCSNL
                     YIALGDADSVINCSFANNTGYTSLGYSDYVVGNIYTENTRPFSISFNPTLVFENNYIY
                     NNTVGLDIYAIDESFFNAAGNYICNNDTNIVFDLAQNKQLLNVCFCETDSATIAATII
                     DGYDDGTLGLLSFAPYTTCDSDIFDDLPEIECASPVIDGINTLTQLPILIYPNPADDF
                     IYLPGLAANSLVYIIDISGKVMLSTQLDATPIIDVSHLARGMYTVITSSGNTIFVKL"
     gene            complement(2894..4099)
                     /locus_tag="PLB46_08550"
     CDS             complement(2894..4099)
                     /locus_tag="PLB46_08550"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF76606.1"
                     /translation="MKVKKIGILREAKFPPDARIPIIPSQIQQLKSGYPEIDIVVQPS
                     KGRCFADYEFEDAGITLQEDLRDCDIIMGVKEVPYDLLIPDKTYLFFSHTIKKQPQNR
                     KLLQQLVKKNIRMIDYECLKDEHGIRVIAFGRWAGIIGAHNALWTLGQRIDEGHLKRA
                     KDCRDYHELKFQYKGLKIPNVKIIVTGTGRVGKGAVEFLDDIKLKRVTKDEFLNYEFN
                     EAVYCVLDSDDLYDRKSDGGFNKEEFHQHPELYKCTFNYYMNKCDVLINCIFWSPAAP
                     QLFSKEDMRNPNWRIKVIADVSCDVNGGVPATIRSTTINEPVYGYDVNTELEIAPYIP
                     ESIDIMAIDNLPNELPRSASEEFGSLLIKSVWAELFKENSRMIHEASITVNGNLGSEF
                     EYLRDYLEG"
     gene            complement(4200..5432)
                     /locus_tag="PLB46_08555"
     CDS             complement(4200..5432)
                     /locus_tag="PLB46_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76607.1"
                     /translation="MMKKTGLILMLWVLSYAAKAQEVRWDTCKFMTYNVLNFDNTGND
                     KEQYLFEVVDSMHPDILIVQELIELSGAIRFFDSVVYKVDTNYAMGTFINSFDTDNGI
                     YFRKDKFEFITNTPITTELRDINEFQLKHIASGEEIYFYSLHLKASSGGTNADQRKRE
                     VDSLRKVTDLLPDTTNFLIVGDFNIYDSNEDAYIALLDQDNSGYVLDPLDGLLTTNWN
                     NSSNAPYHTQSTRIESFGGGSTGGLDDRFDMMLHSQAIEDMGGMTMVAGSYHAIGNDG
                     AHFDDAMNDLPNAVVSASLANALYMSSDHIPLVANYVFEYVADTSGNVDTNVAVIEQK
                     VPQFRVYPNPANEFVTIQSDQIISELAIYAADGKMIFNEAIQSKSATINTNTFPKGIY
                     VAVMQTNAINYRRVFVKN"
     gene            5618..>5989
                     /locus_tag="PLB46_08560"
     CDS             5618..>5989
                     /locus_tag="PLB46_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRF76608.1"
                     /translation="MEISKTYNPAEIEKKWYQHWLDKRYFHSEPDNREAFTIVIPPPN
                     VTGVLHMGHVLNNTIQDVLIRRARMQGKNACWVPGTDHASIATEAKVVKMLRDQGIKK
                     SSISRDKFLEHAWVWKEKYGGI"
BASE COUNT         1919 a         1176 c         1041 g         1853 t
ORIGIN      
        1 ccgatgcagt ttgttcccct gccactaatc agtggcttaa acaagtggta agtggatatg
       61 caacagaaaa caaagcaatt gtgttaggct acggcgcgta tgaaaaaaag cctggccgat
      121 taaacagacg aatccgatac gaaacatttt taacagcctt aaattattta tcatttgcat
      181 tagccaagtt gccatatatg ggggtgggaa gaaatttatc gtaccaaaaa ggtttgtttt
      241 ttagcaccaa tggttttttc gaccatcggc atattccaag tggtgatgat gatttgttta
      301 tcaataaagt cgccactaaa agaaatacag aaatagtaat atctcctgac gcattcactt
      361 actccccacc aaaagaaacc cgtgaggaat ggatagaaca aaaaacacgt catatgagca
      421 ccgccaggta ttaccgggct aaacataaat tctggttggc acttcagcca atttcgcata
      481 ttttgttttg gattacgtcc ctctatttat tggctactgt atttgtttat tggtacatag
      541 ttgcagcagc ttttgttttg cgctgggcac ttttgcgata cacattcgga cagtcgatga
      601 ataaactgga tgaaaaagat ttaaagcctt atatcgaatt atatgatatc atgcaaatcg
      661 gctattattt ccgctttgcc aaagcagctt tagtaaaaac aaaatataga tgggattaaa
      721 cgaaaatctt tctgatcgcg cccgtgagga tatggaactg gttgaaaaag cccgcaaagg
      781 cgaccagtat gcctatagtg ttttgatggc gcgttataaa gattcaatta atttcatgct
      841 gctcaaaatg gtccacaatc gcgatgatgc cgatgattta accatagaag cttttggaaa
      901 agcatttttg catcttgaaa aatatacgcc tgattttgca ttcagcacat ggctgtttaa
      961 aattgccctg aataatgcca tagattttat ccgcaaaaag aaattaaaaa cattgagctt
     1021 agatgatgaa aatgacgaat catcaaaata tgttttttcc aaaattaaaa caagtgcatt
     1081 ggatcctgaa gaatctatta taaaagatca acgcgcaggc ataatgcgca ccattttaga
     1141 tgaactgaat cccaaatata aaacgcttat agaaatgcgg tatttcgaag aactgagtta
     1201 cgaagaaata gccgaaaaac tcaatatgcc actcggcaca gttaaagccc aacttttccg
     1261 cgcacgcaat tttctttacg aattactcgc cgaaagaaaa gggaggattt gatattgaaa
     1321 gtgtattgaa attataacat ggcatgctac tattttgcta aaatagtaag gttatatttt
     1381 aaaatagcat ctcaaaagca attaaggcta aaaatcaata catccaagtt tcctcaccaa
     1441 gaattccaat cgaatgcacc tgtatcacaa tttcacaaaa atggtgttac cggatgatgt
     1501 aataactgta tacatgccac gggctaaatg gctcacatca atgattggtg tggcatcaag
     1561 ttgtgttgaa agcatcactt ttcccgatat atcgataata taaaccaaac tgttcgctgc
     1621 caagcctggt aagtaaatga aatcatcagc aggattggga tatattaaaa ttggcaattg
     1681 tgtcaatgtg tttatgccat caattaccgg actcgcacat tcaatttcag gtaagtcatc
     1741 aaaaatatca ctatcgcatg tggtataggg agcaaaggac aataaaccta aagtaccatc
     1801 atcataacca tcgataattg ttgctgcaat agttgctgaa tcagtttcgc aaaaacatac
     1861 attaagcagc tgtttatttt gagctaaatc aaatacgata ttagtatcat tattacatat
     1921 ataatttccg gctgcattaa aaaatgattc atctattgca tagatgtcta atcctactgt
     1981 attattatag atataattgt tttcaaaaac cagcgtggga ttaaagctaa ttgaaaatgg
     2041 ccttgtgttt tctgtataaa tattacctac aacgtaatcg gaataaccta gggatgtata
     2101 gccggtattg ttagcaaatg aacagttaat aacggaatct gcatcgccta gcgctatata
     2161 taaattacta caaaaatcag tattgataaa tgtgctgtaa ttagtcaacg ctcctccggc
     2221 gttatttgta aaattacaac cttcaaatac tgcaggataa ccttcctggt cgtcgatact
     2281 tacacccgtt tgattgttat caaaagtgca ttgaaaaaaa taacttttcg ggaagtttgt
     2341 tgtcccagtt atgccgtaat aattattttg aaaattacaa tttttacatg tgataacatc
     2401 tgatgctgaa ataggtgtag gaggcaattc gtagtctatc gctttgtggg catcgctgat
     2461 atttacatat tcaaattcga gtgatgaaga atcagttagc agaatacctt cccagcttgt
     2521 ttgtgatggt gttgggttat ttgatttaat tataattggt tggccttcag ttcctattgc
     2581 tgtaaactta ttatccctga tttcaataat gaaaccatca tttactttta cttctacacc
     2641 ggggtttata tttaatgacc ctccagggaa aacggtaaca tttccggtta ttatataagg
     2701 gctttcagcg agtgtccagt tagtaggggt aaaaatgccg ccttcgaaat aagtttgccc
     2761 tatgccaatg ctgaatgcac caaagcataa aacaaatagt aaatgtttta acatatgtgt
     2821 agaattgggt aaaggaatat atggaaagat gaaatttaca aataaaaagt tgcatttcaa
     2881 aagccctttg gtattacccc tccaaataat ctctcaaata ttcaaactca cttcccaggt
     2941 tgccattaac tgttatactc gcttcatgta tcatcctgga gttttctttg aacaattcag
     3001 cccataccga ttttatcaat aatgaaccaa attcttcact cgcgcttctg ggtaattcat
     3061 tcggtaaatt atcaatcgcc ataatgtcaa ttgattcagg aatataaggg gctatttcta
     3121 actcggtatt tacatcatat ccataaacag gttcgttaat ggtggtagac cgaatggttg
     3181 caggcacacc tccgttaacg tcacagctta catctgcaat aactttaatt cgccaatttg
     3241 gattgcgcat atcttccttt gaaaataatt gtggtgcagc aggactccaa aaaatacaat
     3301 taatcaacac atcacattta ttcatgtaat aattaaatgt acatttatat aactcgggat
     3361 gttgatgaaa ttcctcttta ttaaatccac catcgctttt gcggtcatat aaatcatcac
     3421 tatctaaaac acaatatacc gcttcattaa attcataatt taaaaactca tctttggtaa
     3481 cccgttttaa ttttatgtca tccaaaaact caacagctcc tttaccaact cggccggtgc
     3541 cggtgacaat gatttttaca tttggaattt ttaatccttt atattgaaat tttaattcat
     3601 gataatcgcg gcaatctttt gcacgtttta aatgtccttc atcaattcgc tgacccaatg
     3661 tccataatgc attatgcgca ccaattattc ctgcccaacg tccaaatgca attacccgaa
     3721 tgccatgttc atccttcaaa cattcataat caatcatccg gatatttttt ttcaccaatt
     3781 gttgcaacaa ctttctgttt tgtggttgtt tcttaattgt atgagaaaaa aacaggtatg
     3841 ttttatctgg aataagtaaa tcgtaaggta cttcttttac acccattata atatcacagt
     3901 ctcgtaaatc ctcttgtaaa gtaataccgg catcttcaaa ttcataatct gcaaaacaac
     3961 gtcctttact gggttgcact acaatatcga tttcaggata accggatttt aattgctgaa
     4021 tttgacttgg aattatcggt atgcgggcat ccggcggaaa tttcgcctcg cgtaaaatgc
     4081 ctatcttctt aactttcata attggcgtaa agataagcgc ttaatcttaa ggcaaaaatg
     4141 acatctgtcc gtttcacgct taaaacaaac caaattggcc tgattatgcc atcaaaaggc
     4201 taatttttaa caaatactct gcgataatta atcgcattgg tttgcatcac tgcaacataa
     4261 attcctttcg gaaatgtatt ggtattaata gtagctgatt ttgattgaat ggcttcgtta
     4321 aatatcattt tcccatctgc cgcataaatg gccaattcgc tgatgatttg gtccgattgt
     4381 atggtaacaa attcattagc cggattggga taaacacgaa attgtggtac tttttgttca
     4441 attactgcca cattggtatc cacatttcct gaagtatcag ccacgtattc aaaaacgtaa
     4501 ttcgccacca aaggaatatg gtcagaactc atatataacg cattggccag cgatgcactt
     4561 acaacagcgt ttggtaaatc gttcatggca tcatcaaaat gagcaccatc gttgccaatt
     4621 gcatgatagg aacctgcaac catggtcatt ccacccatat cttcaatagc ctggctatgc
     4681 aacatcatat caaaacggtc atctaaacca cctgtcgagc cacccccaaa actttcaata
     4741 cgtgtacttt gggtatggta aggtgcattg gatgaattat tccagttcgt agtgagtaaa
     4801 ccatccaatg ggtcaagtac atatcctgaa ttatcctgat ccagtaacgc gatataagcg
     4861 tcttcgttag aatcgtaaat attaaaatca ccaacaatta aaaagtttgt cgtgtcgggc
     4921 aacaaatcgg taacttttcg caaactgtcc acttcacgtt ttcgctggtc ggcattcgta
     4981 ccaccactgc tggcttttag atgtaaggaa taaaaataaa tttcttcacc actggctata
     5041 tgttttaact gaaattcatt gatgtcccgt aactcagtgg taataggtgt attggtgata
     5101 aattcaaatt tatctttcct gaaataaatg ccattatctg tatcgaaact attgataaat
     5161 gttcccatgg cataattggt atccacctta tataccaccg aatcgaaaaa acgaatggca
     5221 ccggatagtt caattaattc ctgcacaatg agaatatcag gatgcataga atcaactact
     5281 tcaaataagt attgttcttt gtcgttcccc gtgttgtcga aattcaacac attataggtc
     5341 ataaatttac aggtatccca tcgcacttcc tgagccttag ctgcatagct caatacccac
     5401 aacatcaaaa tcagccctgt ttttttcatc attatacaaa gatgctaaaa tgtatggtaa
     5461 aattgagcct ggaacttcaa ttttgggctg atgtcaatca ttagtagcat ttacctaatg
     5521 cttagataaa acaccttcat tcctgacaat atttcttccg ccggccgcaa cacagcctga
     5581 accctcctcc tcatttatta tctttacgcc ctcaattatg gaaatttcta aaacatataa
     5641 tcccgcagaa atagagaaga agtggtatca gcattggctt gacaaacgtt acttccattc
     5701 agaacccgac aatcgtgagg cttttaccat tgtgattccc cctccaaacg taacaggagt
     5761 gctgcatatg ggacatgtgt taaacaatac catacaggat gtacttattc gccgtgcccg
     5821 catgcagggt aaaaatgctt gttgggtgcc cggaaccgac catgcttcga ttgctacgga
     5881 agctaaggtg gtaaaaatgt tgcgcgacca aggtatcaaa aaatccagca tcagccgcga
     5941 taaattttta gaacatgcat gggtttggaa agaaaaatat ggtggcatt
//
LOCUS       DAORKW010000186         5970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_506_length_5970_cov_5.078614, whole genome shotgun sequence.
ACCESSION   DAORKW010000186 DAORKW010000000
VERSION     DAORKW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_506_length_5970_cov_5.078614"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLB46_08565"
     CDS             complement(<1..694)
                     /locus_tag="PLB46_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF76609.1"
                     /translation="MKFNTKVIHAGVDPDPSTGAIMTPIFQTSTYVQNAPGDHKGYEY
                     ARTQNPTRDALQKNLAALENGNFGICFSSGLAATDGIIKMFSQGDEVIAMNDLYGGTY
                     RAFTKVYARFGMQFHFADLTNPNNLEPLINANTKLIWIETPTNPMLSIIDIVAICAIA
                     KKHGVLVCVDNTFASPYLQNPLDLGADIVVHSATKYLGGHSDVIHGCVIVKDKTLADQ
                     LYFIQNATGAVPG"
     gene            complement(962..2206)
                     /locus_tag="PLB46_08570"
     CDS             complement(962..2206)
                     /locus_tag="PLB46_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRF76610.1"
                     /translation="MAIIRSFNGWRPKPEQAAKVASRPYDVLNSEEAREEAAGNDISF
                     LHVCKPEIDLPADIDHYADAVYAKAVENWKRFQSNGTFLQDATPNMYAYRQIMNGHAQ
                     VGLVANSSIQDYFNDIIKKHEYTRPEKENDRIRHMAELQCHPEPVFLTYPDVADIDTI
                     INKVVTSTNPVYDFTAEDGIQHTFWLIDNAQDVQSLTQLFAEKVPFTYIADGHHRSAS
                     SAKVGQAIADKNPNHTGNEEYNFFLSVIFPASQLMIMDYNRVVTDLNGHTSEGILQAV
                     AEKFEVEKRDSIYKPESLHTFGLYLNKTWYKLTAKPGTYNDNDPIDVLDVTIMSNHIL
                     DPILGIKDQRTDKRIDFVGGIRGLGELEKRVNSGEMQLAISLYPVSIQQLINIADSGN
                     VMPPKSTWFEPKLRSGLVVHTF"
     gene            2532..3398
                     /locus_tag="PLB46_08575"
     CDS             2532..3398
                     /locus_tag="PLB46_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76611.1"
                     /translation="MKKLYTPGNESRLKAYSAMALALTAGATTLDATIVYNDIEDVTI
                     EIGDMYDIDIDGDGTLDFHFRAGTIVSSTSSGVWSFGSVFGLYTSYTVGNSGNQLIGY
                     QGSFYNYGSALDEGVTVGPDGPWLSYPSYSNSAVLASNFYGSTFGAFPGQGEKFLGFK
                     FMVGDNTHYGWMRIEADIDPVFITIMDYAYENAPDKEIETGDEESVAINELTSNEVNI
                     YAFGTLVNIINVANLENANATIYDLSGKQVASSALTNGLNQIDLSQLASGNYLVNITC
                     ASGNASKQVFIQ"
     gene            complement(3462..4382)
                     /locus_tag="PLB46_08580"
     CDS             complement(3462..4382)
                     /locus_tag="PLB46_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF76612.1"
                     /translation="MSTILLATEKPFAKDAVEKISKVITNAGHTIQLLENYTDKAQLC
                     EAVKDAAAVIVRSDIVDADVINAGNQLKIVVRAGAGYDNLDLNAATAKNVVCMNTPGQ
                     NANAVAECAFGMMLYMARKQFSGSSGTELRGKRLGIIGYGNIGRYMAQIGRGFGMEVF
                     AYKRNMDRLALQIDGVTPASSFEDIFEHCEYVSLHIPSTAETKGVINMHLLKLLPKNG
                     MLVNTARLDVVNETDLLQLMGERNDITYVSDFVPKMEAAFNEKFAKRIFYPAKKMGAQ
                     TEEANVNAGIAAAKQIIDFLDKGIDTFRVN"
     gene            complement(4455..5522)
                     /gene="serC"
                     /locus_tag="PLB46_08585"
     CDS             complement(4455..5522)
                     /gene="serC"
                     /locus_tag="PLB46_08585"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRF76613.1"
                     /translation="MKVHNFNAGPAILPESVLKQAAQAAIEFNGIGMSILEISHRSKE
                     FQAVMDEAVALTKEIAGLGDEYKVLFLGGGASTQFGMIPMNLLETNETAGYSNTGTWA
                     KKAQKEAKLFGNVAELATSEPDNYTYIPKGYTIPDTLKYFHITSNNTIYGTQYHGYPV
                     SPVPYIIDMSSDMFSRQLDYNQFDLIYAGAQKNIGPAGVTMVIVKESLLGKVNRQIPT
                     MMDYRTHIKEGSMFNTPPVFPIYVTMLTLRWIKERGLAKIEADNKAKADLLYGAIDAN
                     PLFKGTVAKEDRSWMNVNFIMEDRSLEPEFLQFAKENKCIGLAGHRSVGGMRASIYNA
                     LGIESVQFLVNLMDEFAKRKG"
     gene            5902..>5970
                     /locus_tag="PLB46_08590"
     rRNA            5902..>5970
                     /locus_tag="PLB46_08590"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1846 a         1133 c         1074 g         1917 t
ORIGIN      
        1 gccccggcac tgcaccggtt gcattctgta taaaatataa ctgatcggca agggttttat
       61 cttttacaat aacacaaccg tgaattacat cgctatgtcc gcctaaatat tttgttgccg
      121 aatgcactac gatatctgca cctaaatcaa gcgggttttg caaatatggc gaagcaaaag
      181 tattatctac gcaaactaac acaccatgtt ttttagctat tgcacaaata gcaacaatat
      241 caataatact caacatcgga ttggttggtg tttcaatcca aatgagttta gtatttgcat
      301 taataagtgg ttcaaggttg ttaggattgg ttaaatcggc aaaatgaaac tgcatgccaa
      361 atcgcgcata cacttttgta aatgcacgat aggttcctcc gtataagtcg ttcatggcaa
      421 tcacctcatc accctgacta aacattttta taataccatc agtagctgcc aatccgctcg
      481 aaaaacaaat accaaaatta ccgttttcca aagcagccaa atttttctgt aaagcatcac
      541 gcgttggatt ttgtgtgcgc gcatactcat aacctttatg gtcaccgggt gcattttgca
      601 cataagtact agtctgaaaa atcggggtca taatcgcccc tgtgcttgga tcaggatcaa
      661 ctcctgcgtg aatcactttc gtattaaatt tcatgttact tggtttaatt agcaaattag
      721 ctaatgtggt gtagctgcgc tacattatta tgtcaaaaaa atgtcgaagt aagtttaatc
      781 taatcaaccc gctttttata ctgatttagt ttaataatta tagttgcaaa acaatataag
      841 tatttaataa tttagttact gtaagctaag tttaatcagg ttcaatcaac taatacatca
      901 acaccccaaa ttaaatccgc tttatccgcg tcatccgcgg catctgcgtt ccctcttcga
      961 attaaaacgt atggacaacc aatccacttc tcaacttcgg ttcaaaccat gttgattttg
     1021 gaggcatcac atttccgcta tcagcaatat taattaattg ttgaatggaa accggatata
     1081 aactaatcgc caactgcatt tcaccgctgt tcactctttt ttccaattca cctaatccgc
     1141 gaattccacc aacaaaatca atacgcttat cagtacgttg atctttaatt cccagaattg
     1201 gatctaaaat atgattagac ataatagtaa catctaaaac atcaattggg tcgttatcat
     1261 tataagtgcc cggttttgca gttaatttat accaggtttt atttaaatac aatccaaaag
     1321 tgtgtaatga ttcaggttta taaatgctat cgcgtttttc aacttcaaat ttttcagcaa
     1381 cggcttgtaa aataccttca gaagtatgtc cgttcaaatc agttaccaca cgattgtaat
     1441 ccataatcat caactgactt gcagggaaaa ttacacttaa aaagaaatta tattcttcgt
     1501 taccggtatg gtttggattt ttatcagcaa tagcctgtcc aactttagca cttgatgcac
     1561 ttcggtggtg accgtcagct atgtatgtga atggtacttt ttcagcaaat aattgtgtta
     1621 atgactgtac atcttgtgca ttatcaatta accaaaatgt atgctgaata ccatcttctg
     1681 ctgtaaaatc atatacagga ttggtagatg taactacttt attaataata gtatcaatat
     1741 cggcaacatc aggataagtt aagaaaaccg gctcaggatg acattgtaat tctgccatat
     1801 ggcgaatacg gtcgttttct ttttctggac gagtgtattc atgtttttta ataatatcat
     1861 taaaataatc ctgaatagaa gaattcgcaa ctaaaccaac ctgagcatgt ccgttcatta
     1921 tttgacggta agcatacata ttaggtgtgg catcttgtaa aaaagtgcca ttcgactgaa
     1981 aacgtttcca gttttcaact gcttttgcat aaacggcatc ggcataatga tcaatatcgg
     2041 caggtaaatc aatttccggt ttacaaacat gtaaaaatga aatatcgtta ccggcagctt
     2101 cttcgcgcgc ttcttcactg tttaaaacat cgtaaggacg gcttgctact ttggcagctt
     2161 gctccggctt tggtctccag ccgttgaatg atcttattat ggccatagtt gtattttttg
     2221 cgcaaagata gcagaactgt tttcaattat gccactagtt gaccaaatgc ccctttttga
     2281 cttacgcggt aaggttttgt tgcttttatt gggatgtagt atcaatgctc cttctgcgct
     2341 aaccttttcc tgaaaatgct gtaaagcaat gtgttagcgt gttttaatta gggggagatg
     2401 tcatttacag cgggcatgtg aagatttttt cagacattag aattaacctt agttaacaac
     2461 aattacggtc tctataggat ggtaagtccg aaaaaattac tacttttagc tacaaattaa
     2521 taattcaaaa tatgaagaaa ctttacacgc caggtaatga aagcagatta aaagcctact
     2581 cggcaatggc attagcacta actgcaggtg caacaacatt agatgcaacc attgtttaca
     2641 acgacattga agatgtaaca atcgaaatcg gcgatatgta tgatatcgat atcgatggtg
     2701 atggcacact cgatttccac tttagagctg gaactattgt aagttcaacc agcagcggtg
     2761 tttggtcgtt cggttcagta tttggccttt acacatctta tacagttgga aattctggca
     2821 atcaattaat cggttatcaa ggtagtttct ataattatgg tagtgcttta gatgaaggtg
     2881 taacagttgg tcctgatggt ccatggttat cttatcctag ctatagcaac tctgcagttt
     2941 tagcttctaa cttctatggc agcacattcg gtgccttccc tggtcagggc gaaaaattct
     3001 taggatttaa atttatggtt ggcgataata cacattatgg ctggatgaga attgaagctg
     3061 atatcgatcc tgtgtttatc actattatgg attatgctta cgaaaatgca cctgataaag
     3121 aaattgaaac cggtgatgaa gaatcagttg caattaatga attaacatca aacgaagtaa
     3181 atatttatgc tttcggaaca ttggtgaata ttattaatgt agcgaattta gaaaatgcca
     3241 atgctactat ttatgattta agtggtaaac aagttgcttc atcagcgtta acaaatggat
     3301 tgaatcaaat tgacttgtca caattagcaa gcggcaatta tttggtaaat attacctgtg
     3361 caagcggcaa tgcttcaaaa caagtattta ttcaataatc aaataataag tatcaacaaa
     3421 aatgtccgca attgcggaca tttttgtttt gatagaatag cttaattcac tctgaaagta
     3481 tcaatacctt tatcaaggaa atcgattatt tgtttagccg cagcaatacc tgcgtttaca
     3541 tttgcttcct ctgtttgtgc acccattttt ttagcaggat aaaaaatacg tttagcgaat
     3601 ttttcattaa atgctgcttc cattttagga acaaaatcag aaacataggt aatatcgttg
     3661 cgctcaccca ttaattgtaa caagtcagtt tcattaacaa cgtctaatcg tgcagtatta
     3721 actaacatac catttttagg taataatttt aataaatgca tattaataac acctttggtt
     3781 tcggcagtag aaggaatatg cagcgaaaca tattcgcaat gttcgaaaat atcttcaaaa
     3841 ctgcttgcag gtgtaacacc atcaatttgt aatgcaaggc gatccatgtt gcgtttgtaa
     3901 gcaaaaacct ccataccgaa tcctctgcca atttgcgcca tgtatctacc aatattacca
     3961 tatccgataa tacccaatct tttcccacgt agttcggtac ctgaactccc tgaaaattgt
     4021 ttacgcgcca tatataacat catgccaaat gcacactcag caactgcatt tgcattttga
     4081 cccggtgtgt tcatgcatac cacatttttt gcagtagcag catttaaatc taaattgtcg
     4141 tagcctgcac ctgcgcgaac aacaattttt aattgattac cggcattaat tacgtcggca
     4201 tcaacaatat cacttcttac tataacagct gcagcatctt taacggcttc gcataattga
     4261 gctttgtcag tataattttc taataactgg atcgtatggc ctgcgtttgt aattacttta
     4321 cttatttttt ctacagcatc ttttgcaaac ggtttttcgg ttgctaataa aatagtactc
     4381 ataaaattgg aggtttaata tttgaagaga taaaaaaagt tgccactaaa tttgaagtgg
     4441 caactcgaaa attattatcc tttgcgttta gcaaattcat ccataagatt aactaagaac
     4501 tgaacgcttt caattccgag tgcattatat atagatgcgc gcatgcctcc aacacttctg
     4561 tgaccggcaa ggccaataca tttgttttct ttagcaaatt gtaaaaattc aggttcaagt
     4621 gaacgatctt ccataataaa gtttacgttc atccagctgc ggtcttcttt agcaacggtg
     4681 cctttaaata aaggattagc atctatggca ccatataata aatctgcctt agccttattg
     4741 tccgcttcaa tttttgccaa accgcgttct ttaatccaac gcaatgtgag catggtaaca
     4801 tatataggga ataccggagg agtattaaac atactacctt ctttaatatg cgtgcggtaa
     4861 tccatcatgg tagggatttg tctgttcact ttacctaaca ggctttcttt aacaatcacc
     4921 atagttacac cggcagggcc gatatttttc tgagcacctg cataaatgag gtcgaactga
     4981 ttataatcca attgacgcga aaacatatca gagctcatat ctattatata aggcaccggt
     5041 gaaacagggt agccatggta ttgagtcccg tatatagtat tgttagatgt gatatggaaa
     5101 tactttaaag tatcagggat tgtatatcct ttaggtatat aagtatagtt atccggttca
     5161 gacgtggcga gttcagcaac attaccaaac agtttggctt ccttttgagc ttttttagcc
     5221 caggttccgg tatttgaata accggcagtt tcgttggttt caagcaagtt catcggaatc
     5281 ataccgaatt gggtgcttgc ccctccgccg aggaaaagta ctttgtattc atcccccaat
     5341 ccggcaattt ctttggtaag cgcaacggct tcatccataa ctgcctgaaa ttcttttgag
     5401 cggtgagaga tttcaagaat cgacattcct atgccgttaa actcaatagc agcttgggct
     5461 gcttgcttta gcacggactc aggaagtatg gctgggccgg cattaaaatt gtgaactttc
     5521 atattttttt taagatttat tttgatatgt aaaattaatc actacctttg cactcccaat
     5581 tcgttcttta caccaagata gcaaccgaga aagcgggtca aaattacaat ttccgcctat
     5641 aagaagtagc aatatttcaa aaaataggta aagtgataaa aagcagtgat gacacgggtt
     5701 ttaaaggaaa ataaaaaaaa gttatatttt tttgcttaaa aatttgaatt tacgatttcg
     5761 gttatgttac ctttgtgtcc catttgaaaa aagagggctg tcggaaagac agatttttta
     5821 taagttcttt gaagtattgg agaaacaact agtagcaagt tcaggataaa taaggagcca
     5881 cagtaattac aaaaattttt tacaatggag agtttgatcc tggctcagga tgaacgctag
     5941 cggcaggctt aatacatgca agtcgagggg
//
LOCUS       DAORKW010000187         5939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_509_length_5939_cov_4.430489, whole genome shotgun sequence.
ACCESSION   DAORKW010000187 DAORKW010000000
VERSION     DAORKW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_509_length_5939_cov_4.430489"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLB46_08595"
     CDS             <1..488
                     /locus_tag="PLB46_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76614.1"
                     /translation="WYLLGGSRSGNVHQYNEIEEALLGEFFETTDNFYQYDAGDFVAL
                     NGADINNDGNMEFITGNVRGGFQIFEQDKFTPIVSSENPTLLLIPNPTSDQIHITLNN
                     FSGANSTITIYNIAGEKLLHLNNNDSHISITLPGTFLPGMYFVTVATNQNLVTGTFIK
                     L"
     gene            491..928
                     /locus_tag="PLB46_08600"
     CDS             491..928
                     /locus_tag="PLB46_08600"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRF76615.1"
                     /translation="METVHWKKLENLFLRANIQQLYTGISIQVSDNEACISWPVDSSH
                     FHGANALHGASYFKLLDDAAYFAAASKHTSTFLVTGSYSLKFLKPVHAGIITAKGLAN
                     ESAGKYIKSSSRLFNEKGDLLAEGSGIFIPTATLWSSLKAYQL"
     gene            1006..1389
                     /locus_tag="PLB46_08605"
     CDS             1006..1389
                     /locus_tag="PLB46_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76616.1"
                     /translation="MKKLATSILFLFVCTLAVKAVGPDKATIATMNEKMVITLPTNVP
                     LSDIYLIDISAIPFSSEALLSGFCQSFSENHMTLTGNFSTKQITLSLHSTEEVAGEKW
                     DLIKWNEYLSSRAPKMLMYVNTQNR"
     gene            1411..3933
                     /locus_tag="PLB46_08610"
     CDS             1411..3933
                     /locus_tag="PLB46_08610"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRF76617.1"
                     /translation="MRLITLPLLFGLTLSVAQAQYCIPDYTVGTTDDDYINGVEINTI
                     SNLDSGPGDGSGYSDFTDLTTDLFIGISYDVHIANTPDYTEGYRIWIDYNQDEIFADD
                     EQIFEPFILEEEFETIMPFTVPLDAAIGPTRMRVRCSYNAIDFDACSGETYGEAEDYT
                     INILGVSNDIGAISILDLANNCEFTTNETITIAVENFGTESASDFEVAYSIDGGSPVV
                     ELFPALLHIDETLPYSFATTADLSADGIHIIKVWTNWVDDAYNGNDTATIYLLNDYTV
                     LTTGFPENLCYFGTTVLPTGVPDGGVWSGEGIINPTTGELDPAIVGGIGGSTIVEYAY
                     TATDKYAVFEIPYFLTPMTSPTILALGDEDFESGIPLGFNFEYFDETYSEIFISSNGF
                     IGFGAGSTSYDVQHIPNTSNPDNIIALAWSDLDPTAGGTISYELQGVAPERKFIVSYK
                     NVFHYLSDATISGQIILYENGNIIDIVNTLIESDGGVVTQGIENLNGTVAYVGDEDYN
                     KDVFTITTESWRYYYTPCDAVVTDTITFIAAPEVSIADSSACVGATINLDAGEGAAFY
                     VWSTGSDAQSIDVTSSGTYSVTYYANESCYVSDSAVITIYPNPVIDLGEDGGICEGAY
                     LNAENTGADYLWNTGDTTQTLFISTSGTYYVDVVSAAGCANSDTITLSITPLPTADFD
                     VITGEGLIAVFTSFATDAVTWLWDFGDGTISTIENPDHTYPTDGSYVVTLVVTNDCGA
                     DIYTTTIQVTTGINTQHFVDGGLTIFPNPASTSITVDLLISQTGLPSSIKIYDMTGNI
                     VANMEQVFGEQISLPITNLSSGNYMVVATNGIKTAMSSFIKN"
     gene            complement(3938..4804)
                     /locus_tag="PLB46_08615"
     CDS             complement(3938..4804)
                     /locus_tag="PLB46_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF76618.1"
                     /translation="MNALKILFTGIGCITTIVVSAQISVAVYFNSGSANNGINLQHTL
                     DSTITFLHTQQIDHIDLHAYCDTDGDSTFNMRLSEKRNAFVKKVLQNSGIQDSIFSDI
                     NAFGENNATQENNDEIAKQQNRRVEIIIHPAANNPNIAADNLNSITAMATAILNAEIG
                     QKVVIPNLEFFSGRHTLMPESQPVLQELVKVMQENPNLYISLIGHICCMTEMQKQMGA
                     YRDGFDADDGTNHLSLNRAIVVKTYLTDFGIASERILVKGKGGAEKLIDPEITEADKK
                     RNRRVEVMIIAK"
     gene            complement(4860..5381)
                     /locus_tag="PLB46_08620"
     CDS             complement(4860..5381)
                     /locus_tag="PLB46_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76619.1"
                     /translation="MDAITFTIVLGATAGFLGGLLNTLLWRYILKHTHFAGIQLHEGF
                     SEMIQSALHADNNTNKLDGFAALEKNILMLFDDFMVNKLTVKMPVLSMFIDDKLIEEI
                     RVVFRDEMSANLPQLLQNTSTSKTASNMMDNMSNHILQHISARFKRFAYLFIAVTTFV
                     GAITGLIIFSFVQ"
     gene            complement(5369..>5939)
                     /gene="pyrE"
                     /locus_tag="PLB46_08625"
     CDS             complement(5369..>5939)
                     /gene="pyrE"
                     /locus_tag="PLB46_08625"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRF76620.1"
                     /translation="PNEPFTGASGWRSPIYCDNRLTLSYPEIRTWLKNTLAAGISQTF
                     PNVEIIAGVATAGIPHGALVAEALNLPFIYVRSEAKKHGLSNQIEGKLPEGAKVMVIE
                     DLVSTGGSSLTAVNALRNAGAQVEGMAALFTYAFDAADEAFKNADCLLYTLSDYPSLI
                     QYALANNIIPKETESLLGEWRKNPGAWMQ"
BASE COUNT         1805 a         1077 c         1132 g         1925 t
ORIGIN      
        1 catggtattt attaggtggt tctcgaagtg gcaatgtgca tcaatataat gaaattgaag
       61 aagcattatt gggcgaattt tttgaaacaa ccgataactt ttaccaatat gatgcaggag
      121 attttgttgc attaaatggt gctgacatta ataatgatgg caatatggaa tttattaccg
      181 gaaatgtaag aggtggtttt caaatttttg agcaggataa attcacgcca attgtatcat
      241 ctgaaaatcc aactttattg ttaattccga acccaaccag cgaccaaatc catatcacct
      301 taaataactt ttcaggtgca aacagtacaa taactattta caatatagcg ggtgaaaaat
      361 tgttacatct caacaataac gacagccata tcagtataac attgccggga acatttttac
      421 cgggtatgta ctttgtaact gttgctacaa atcaaaattt ggttaccgga acttttatta
      481 aattataaag atggaaaccg tgcattggaa aaaactggaa aatttgtttt tacgggcaaa
      541 cattcagcaa ttgtataccg gtataagtat acaagtttct gataatgaag cctgtatttc
      601 ctggccggta gactcaagcc attttcatgg tgcaaatgca ttacatggcg cttcgtattt
      661 caagctatta gatgatgccg catattttgc tgctgcttct aaacacacgt ccacctttct
      721 tgtgacgggt tcatattcct taaaattctt aaaacctgtt catgccggaa ttattacggc
      781 aaaaggatta gcaaacgaat cggcaggtaa gtacattaaa tcttcaagcc gtttgtttaa
      841 tgaaaagggt gatttgctcg ccgaaggatc tggtatattc attccaactg ctacattgtg
      901 gtccagcctg aaggcttatc aattgtgatt tgtcacgaat tcgggttttt tgtatacttt
      961 ttactgacag atttttctat ctttaacaca aataaacgat tacaaatgaa aaaattagct
     1021 acttcaatcc tctttttatt cgtatgtact ttggcagtta aggcggttgg accagataaa
     1081 gcaaccattg cgaccatgaa tgaaaaaatg gtaattaccc ttccaaccaa tgtgccgctt
     1141 tcagatatct accttataga tatctctgca attcctttta gtagtgaagc attattaagt
     1201 ggattttgtc aaagttttag tgaaaatcat atgacgttaa ctggtaattt ttcaacaaaa
     1261 caaattacac tttcacttca ttccactgaa gaagtagcag gtgaaaaatg ggatttaatc
     1321 aagtggaatg aatatttaag ttcccgtgct cctaagatgt tgatgtatgt gaatacccaa
     1381 aatagataat gctgttataa attttttcct ttgcgattaa ttaccctgcc cctcctattt
     1441 gggttaacgc tatctgttgc ccaagcccaa tattgtattc ctgattatac cgttgggacc
     1501 actgatgatg attatatcaa tggtgttgaa ataaacacta ttagcaacct ggatagcggc
     1561 cccggtgatg gatcgggata cagtgatttc acggatttaa ctacggattt atttatcggt
     1621 atttcatacg atgtacatat agccaataca ccggattaca cagaaggtta ccgaatttgg
     1681 atcgattaca atcaggatga aatattcgct gatgatgagc agatatttga gccatttata
     1741 ttggaagagg aatttgaaac aataatgcca tttacagtgc ctttagacgc cgccatcggg
     1801 cctaccagaa tgcgtgttcg atgttcatat aatgctattg attttgatgc atgtagtggc
     1861 gaaacttatg gtgaagcaga agactatacc ataaatattt taggtgtttc taatgatatt
     1921 ggcgctatca gtattttgga tttagctaac aattgtgaat ttaccacaaa tgaaaccatt
     1981 acaattgcag tagaaaattt tggtactgaa agtgcaagcg attttgaagt ggcttattca
     2041 attgatggtg gctcaccggt tgttgaatta tttcctgcat tattacatat tgatgaaact
     2101 ttaccttatt catttgcaac aactgccgat ttatccgcag atggtataca tatcataaaa
     2161 gtttggacaa attgggtgga tgatgcttat aatggtaatg acactgccac tatttattta
     2221 ttgaatgatt acactgtttt aacaacaggc ttccctgaaa atctttgtta tttcgggaca
     2281 actgttttac cgacaggtgt tccggatggt ggcgtttggt ccggtgaagg tataattaat
     2341 ccaaccaccg gcgaattgga cccagcaatt gttggtggca ttggtggttc tactattgtt
     2401 gaatacgcgt atactgcaac agacaaatat gctgtttttg aaattcctta tttcttaaca
     2461 cccatgacat ctcctaccat attagcactg ggagatgaag atttcgaatc cggaataccc
     2521 ttaggtttta atttcgaata ttttgacgaa acctattcag agatttttat ttcctcaaat
     2581 ggatttatag gttttggtgc gggctcaact tcgtatgatg tgcagcatat accaaatacg
     2641 agtaatcccg acaatataat tgcccttgca tggagtgatt tagacccaac tgctggtgga
     2701 acaatttctt atgagctgca aggtgttgct cctgaaagaa aatttattgt tagttataag
     2761 aatgtatttc attatttaag tgatgctaca atttcaggcc aaattatttt atatgaaaac
     2821 gggaatatca tcgatattgt aaatacctta attgaaagtg acggaggagt tgtaactcaa
     2881 ggtatagaaa atttaaatgg cacagtggct tatgttggtg acgaagatta taataaggat
     2941 gtatttacta ttaccactga aagctggaga tattattata caccttgtga tgcagttgtt
     3001 acagatacca ttacatttat tgccgcacct gaagttagta ttgcagattc aagtgcatgt
     3061 gttggtgcca ccataaattt agatgccggt gaaggagcag cgttttatgt ttggagtacc
     3121 ggctcggatg ctcaaagtat tgacgtaacc agcagtggta cttattcagt aacttattat
     3181 gcgaatgaat cttgttatgt tagcgattct gctgtaataa ccatttatcc caaccccgtt
     3241 attgatttag gggaagatgg tggcatatgt gaaggcgcat accttaatgc agaaaatacc
     3301 ggggccgatt atttatggaa tacaggtgat accacacaaa cattatttat tagcacatca
     3361 ggaacgtatt atgtagatgt tgtaagcgcc gcaggatgtg ccaacagcga taccattacc
     3421 ttgtcgatta caccattacc aactgctgac tttgatgtaa taacaggaga aggtttaatt
     3481 gctgtattta ctagcttcgc gactgatgca gttacatggc tttgggattt tggggatggc
     3541 actatttcaa caattgaaaa tcctgatcac acgtatccaa ctgacggtag ctatgttgta
     3601 acactggtgg ttaccaacga ttgtggtgca gatatttata ccactaccat tcaggttact
     3661 accggaatta atactcagca ttttgtagat ggcggattga ccatttttcc aaatcctgca
     3721 tcaacatcaa ttactgttga tttattaata agccaaacag gattgccttc ttcaattaaa
     3781 atttacgata tgacaggtaa tattgtagct aatatggagc aggtttttgg agagcaaatc
     3841 agtttaccca ttacaaattt atcttccggg aattatatgg tagtggcaac aaatggtatt
     3901 aaaacagcga tgagtagttt tattaaaaac taaattttta tttagcaatt atcattactt
     3961 ctactcttct gtttcgtttt ttgtccgctt cggtaatttc ggggtcaatt aatttttcgg
     4021 cgccaccttt accttttacc aaaatacgtt ctgaggcaat tccaaaatcg gtgagataag
     4081 tttttacaac tatagctctg tttaacgaaa ggtggttggt gccatcatca gcatcaaatc
     4141 catcgcgata tgcacccatt tgtttttgca tttctgtcat acaacatata tgacctatta
     4201 atgaaatata caggttagga ttttcttgca tcacttttac taattcctgc aatacgggct
     4261 gactttcagg cataagtgta tgcctgccgg aaaaaaattc taaattggga attactactt
     4321 tttgtccgat ttcagcattc aaaattgcag tagccattgc cgtaatagaa tttaaattat
     4381 ctgcagcaat gtttggatta tttgctgccg ggtgaataat aatttccacc ctcctatttt
     4441 gttgtttggc tatctcatca ttattttcct gtgtagcatt attttcgcca aaggcattta
     4501 tgtccgaaaa aattgaatcc tgaattccgg aattttgtaa tactttttta acaaaagcat
     4561 tccttttttc cgacaatcgc atattgaaag tactatcacc gtcagtatcg caataagcat
     4621 gtaaatcaat atggtcaatt tgctgagtat gcaaaaaggt tatggtagaa tcaagtgtgt
     4681 gttgcaggtt gataccatta tttgcgctac cgctattaaa ataaacagca acagaaattt
     4741 gagctgaaac aacaatagtt gtaatacaac caataccggt aaaaagtatt ttcagggcgt
     4801 tcatcactaa taaacgaaat tactgaaaat ctattgagtg ttacttcaaa taaccttatt
     4861 tattgcacaa aagaaaagat aattaaaccg gtgatagcgc cgacaaaggt ggtaacggca
     4921 ataaacagat aagcaaatcg tttgaacctt gctgaaatgt gctggagtat gtggttcgac
     4981 atattgtcca tcatgtttga agccgttttg gatgttgacg tattttgtaa caattgcggt
     5041 aagttggcgg acatttcatc ccgaaataca actcgtattt cttcgattaa tttatcatca
     5101 ataaacatgc tgagcaccgg cattttaact gtcagtttat ttaccataaa atcatcaaat
     5161 aacattagga tatttttttc caatgctgca aaaccatcca gtttatttgt attgttatca
     5221 gcatgtaaag ctgattgaat catttcagaa aaaccttcat gcagttgtat acctgcaaaa
     5281 tgggtatgct ttaaaatata tcgccataat aaggtattta ataatcctcc taaaaaccct
     5341 gcagtggcac ccagcactat ggtaaatgtt attgcatcca tgcgcccggg tttttgcgcc
     5401 attcacctaa aagcgattct gtttctttag gaataatatt atttgcgaga gcatattgaa
     5461 ttaaagacgg ataatcggac aaagtatata aaaggcaatc cgcattttta aaagcttcat
     5521 ctgcagcatc aaatgcatag gtaaacaacg cagccatacc ttccacctgc gcacctgcat
     5581 tgcgtaaggc attcaccgca gttaaactac ttccaccggt ggacacaagg tcctcaataa
     5641 ccatcacttt agctccttcc ggcaattttc cttcaatttg gttgcttagt ccgtgttttt
     5701 tagcctcaga acgcacataa ataaaaggca gattgagtgc ttctgcaacc agtgcaccat
     5761 gcggaattcc ggctgttgcc actccggcaa tgatttccac attaggaaag gtttgactta
     5821 tccccgcagc tagcgtattt ttgagccagg ttcttatttc cgggtaggac aaggttaacc
     5881 gattatcgca ataaatcggc gatcgccagc cactagcccc ggtaaatggt tcattcggg
//
LOCUS       DAORKW010000188         5937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_510_length_5937_cov_4.397484, whole genome shotgun sequence.
ACCESSION   DAORKW010000188 DAORKW010000000
VERSION     DAORKW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_510_length_5937_cov_4.397484"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..2004
                     /locus_tag="PLB46_08630"
     CDS             115..2004
                     /locus_tag="PLB46_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HRF76621.1"
                     /translation="MQFKNLQQFIDLLEKEGELLRIKEFVNPNLEITEIADRFIKNDG
                     PALLFENTGTDFPLLINSMGSYKRMCMALGVNELDDIGNDIEALFKKMVAPKNSLMEK
                     VMMLPELGKVASYMPKTLKGKGECQQVIMDDPDITKLPVMTCWPADGGPFITLPVIHT
                     LDPENNTRNVGMYRMQVFGPKLTGMHWHKHKVSAGHFNKYKKLGKRMPVSVALGGDPV
                     YTYCATAPLPPNVDEYLLAGFIRKRKVELVKCITNVLYVPADADFIIEGYVDPDEDFI
                     FEGPFGDHTGYYSLADYYPKFHITCITHKKNAVYPSTIVGIPPQEDAWIGKATERIFI
                     TPIKLTMLPEVEDMELPIEGVFHNLTIVKIKTEYEGHAQKVMNAMWGAGQMMFNKILV
                     ITDKIDIHNYAQVADYVSKHVDPYNDIYFSAGPMDVLDHSCSKFAFGSKVCIDATEKT
                     DAHVLVDTRSIIIDTPALQQKYPAISGINTQLLQAGFSVIAVGVKKTLPAQIETLHKQ
                     LVLEEGVNQVKFIIYVDDVVELSDFGVVTWNTANNIDPRRDMILSPAGKYGISHCGID
                     GTRKTKKLDNFQRDWPNVIAMDAAIIETIDKKWEVLGLGKFLPSPSLKYVGQVMNKGA
                     VVIEN"
     gene            2120..2956
                     /locus_tag="PLB46_08635"
     CDS             2120..2956
                     /locus_tag="PLB46_08635"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRF76622.1"
                     /translation="MQSLVQYFETIPALHRSLILFGGMLFFYLIEYAVPLFKFNYRKL
                     HHAGINLLFTLTTIIINFLFAFTIVKIGDWSIQQHIGILTFLDVNLLWYTIIGLLLMD
                     LIGAYTVHYVEHKVKWMWRFHLIHHSDTHIDTTSANRHHPGESVFRLFFTVIAVGIIG
                     APIWLVMMYQSASVVLSQFNHANIRLPLWLDKTLSWIIVSPNMHKVHHHYVQPYTDSN
                     YGNIFSIWDRIFGTYTTLAPEKITYGIDTHMLPEENNRVGNLFAIPFQPYRAPKGSKF
                     NE"
     gene            complement(3278..3835)
                     /locus_tag="PLB46_08640"
     CDS             complement(3278..3835)
                     /locus_tag="PLB46_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRF76623.1"
                     /translation="MKGGYIIRDPYAKHFVTLSVVYWIDIFSRQLYRDIVIDSFKYCQ
                     EHMGLTINGYVIMSNHIHAILQSQNGKLSQTITSFKKFTGAKFIQSIFDCPESRRVWL
                     LNQFAHASIVHNRNSSHQIWVNGNRPMSLTTGTFFWQKLNYIHNNPVRAGIVDNPEDY
                     IYSSARNYLGREGKLKIELVDWVKW"
     gene            complement(4405..>5937)
                     /locus_tag="PLB46_08645"
     CDS             complement(4405..>5937)
                     /locus_tag="PLB46_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF76624.1"
                     /translation="VANIKDVTSFSAPELFKGPFNSAYNDGTLAFNKDYSEVYFTRCG
                     TDDRKVDDYCGIYVSNYLPDGGWSEPVALPFFDDTLNIGTPCLSPDGNTLFFAATNPD
                     GYGGADIYMSRRTESGWDAAYNAGQVINTNGNEVFPYFDSDGVMYFSSDKHPGLGGLD
                     IFSATWKNNKFSNIKNMEYPINSGADDFGLIMLDKKQFANSDTLSAGYFSSNRKGGMG
                     DDDMYFFAKTTKKLRPPVFVLKGTVLEKVYLDSLDVNSTVLDTIPLDKSIATLSYPNE
                     QTLLGKWNIGDEGIFSIQVDSLKDYKITGMKEGYFNNTTYVTTKTVKGKPGDTVFVYA
                     SVVLDKIPIASGEKAGEIKLKNIYYDYNDSSLRAESFPELDKLVKLLNENPGLNIQLN
                     SHTDSRGVDKYNQKLSRGRANSVVKYLIEKGIAAERLTFKGFGESSPSTLTTDEILPD
                     GKVVPKGSVLTEAFINTYKSNKDNFEYLHQLNRRTTFQVTSATMNIESEDADEIIIDK
                     AD"
BASE COUNT         1835 a         1100 c         1096 g         1906 t
ORIGIN      
        1 ctgtttcatt ggtacatcgg tacattccca cattggtaca ttaatcagca catcagcaca
       61 ttaacaaatc agcacattaa ctcaaccttc tttccttaac tttgttccac tattatgcaa
      121 ttcaaaaatt tacaacaatt tatcgacctg cttgaaaaag aaggggagct gctgcgcatt
      181 aaggagtttg ttaatccgaa tctcgaaatt accgaaatcg cagaccggtt tatcaaaaac
      241 gacggacctg ctttgttgtt cgaaaatacc ggaaccgatt ttcctttgct cattaatagt
      301 atgggctcct ataagcgaat gtgtatggct ttgggggtta atgaactcga tgatattggt
      361 aatgatattg aagccttatt taaaaaaatg gtggctccga aaaatagttt aatggagaaa
      421 gtaatgatgt tgccagagtt aggtaaagta gcatcttaca tgcccaaaac cttgaaagga
      481 aaaggcgaat gtcagcaggt aattatggac gacccggata taactaaatt gccggtgatg
      541 acttgctggc cagccgatgg cggaccattt attacacttc ctgtaattca taccttagat
      601 cctgaaaaca atacacgaaa tgtaggcatg tatcgcatgc aggtattcgg accaaaatta
      661 acaggtatgc attggcataa acataaagta agtgccgggc attttaataa atataaaaaa
      721 ttaggtaaac gtatgccggt ttcggtagca ttaggtggtg accctgttta tacctattgc
      781 gcaactgcac ctttaccgcc aaatgtggat gaatatttat tagccggttt tatcagaaaa
      841 agaaaagtgg aattggtaaa atgtattacg aacgttttat atgttcctgc cgatgccgat
      901 tttattattg aaggttatgt ggaccctgat gaagatttta tttttgaagg accatttggc
      961 gatcataccg gatattattc gttagcagat tattatccca aatttcatat tacctgcatt
     1021 acgcataaaa aaaatgccgt atatccatca actatagttg gtattccacc gcaggaagat
     1081 gcatggattg gcaaagcaac tgaacgcatt tttattacgc caattaaatt aaccatgttg
     1141 cccgaagtag aggacatgga attaccaatt gaaggtgttt ttcataacct gactattgta
     1201 aaaattaaaa cggaatatga aggccatgca caaaaagtaa tgaatgccat gtggggtgcc
     1261 gggcaaatga tgtttaataa aatattggta ataaccgata aaatagatat tcataattac
     1321 gcgcaagtgg ccgattatgt cagcaaacat gtagaccctt acaacgatat ttatttttcc
     1381 gcggggccaa tggatgtgct ggatcacagt tgctccaaat ttgccttcgg ttcaaaagtg
     1441 tgtatcgatg caaccgaaaa aacagatgcg catgttttgg ttgatacacg ttcaataatt
     1501 attgataccc cggctttaca acaaaaatac cctgcaattt ccggcattaa tactcaatta
     1561 ttacaagcag gtttttctgt aatcgctgtt ggtgtgaaaa aaacattacc agcacaaatc
     1621 gaaactttgc ataaacaatt ggtattagaa gaaggtgtta atcaggtgaa gtttattatt
     1681 tatgttgatg atgtagttga gttaagtgat tttggtgtag ttacctggaa cacagccaat
     1741 aatatcgacc cgcggcgcga tatgatatta tctccggcag gtaaatatgg tatttcacat
     1801 tgcggaattg acggcacgcg caaaactaaa aaactggata attttcagcg cgactggcct
     1861 aatgtaattg ctatggatgc tgcaataatt gaaaccatcg acaagaaatg ggaagtatta
     1921 ggtttaggta aatttttgcc ttcgccttcg cttaaatatg tgggacaggt gatgaataaa
     1981 ggcgctgtgg taattgaaaa ttaatacttg ggtttgcttt acgtgtttga ttaattttta
     2041 atgtgttttt agccggttgt ttggttaata agttattatt aacttggctc atcatttatt
     2101 caccctcaac cttaaacgta tgcaaagcct ggtgcaatat tttgaaacta ttcccgcatt
     2161 acaccgatcc ttgattttat ttgggggcat gttgtttttt tacctgattg aatatgctgt
     2221 gccattattc aaattcaact atcgcaaatt acatcatgca ggcatcaatt tgttattcac
     2281 attaacgact attattatca attttttgtt tgcctttacc atcgtaaaaa ttggtgattg
     2341 gtcaattcaa caacatatag gaattttaac ttttttagat gtcaatctgc tttggtatac
     2401 tattatcggt ttattgctaa tggatttgat tggtgcatac acagttcatt acgtggaaca
     2461 caaagtaaaa tggatgtggc ggtttcatct tattcatcat tcagatacgc atatcgatac
     2521 aacatctgcc aatcgtcacc accctggaga aagtgttttt cgtttatttt ttacagtaat
     2581 tgctgtcgga ataattggag cgccaatttg gttagttatg atgtatcaat cagcttcggt
     2641 ggtattatcc caatttaatc atgccaatat tcgattgccg ctatggttgg ataaaacatt
     2701 gagttggatt attgtttcgc caaatatgca taaagtacac catcattatg ttcagccata
     2761 tactgattct aactacggca atattttttc catttgggat cgtattttcg gcacctatac
     2821 tacattagca cctgaaaaaa tcacctacgg aattgatacg catatgttac ctgaagaaaa
     2881 caatcgtgta ggtaatttat ttgccattcc tttccagcca tatcgtgcgc caaaggggag
     2941 taagtttaat gaataaacat tctcgcttcg aatattaatc cacgatcgcg gacaggagtc
     3001 cggggtggcg tctggagttg aaaactccac gacaacgcga agtcttcgac ttcttttgtg
     3061 tagggcgtag ggttggtttg gcgaatgtca ggtcattgat ataagcaaat aacaaatgaa
     3121 gtcttagact tcatttgacg aattaataat taaaattcta atttagaatt cccatggata
     3181 gcactaaccc accctttgtg tcgcagacac aaagttgtcg tggagttttc aactccagac
     3241 gccaccccgg tctcccgacc ggcgaaaaaa tgaatggtta ccacttaacc caatcaacta
     3301 gctcaatttt taatttacct tccctgccca aataattcct tgcactcgaa taaatataat
     3361 cctccggatt atcaacaatc ccagcacgca ccggattatt atgaatataa tttaatttct
     3421 gccaaaaaaa tgttccggtt gttaaggaca taggcctgtt gccattcacc caaatttgat
     3481 gactgctatt tctattatga acaattgaag catgtgcaaa ctgatttaat agccaaactc
     3541 gtctgctttc agggcaatca aatattgatt gaataaactt ggcgccggta aattttttaa
     3601 atgatgttat tgtttgcgaa agtttcccgt tttgactttg taaaatggca tgaatatgat
     3661 tagacattat cacatatcca tttattgtta atcccatatg ctcctggcaa tatttgaaac
     3721 tatcaattac aatatctcta tacaattgcc tgctaaaaat atcaatccaa tagaccacac
     3781 ttagtgttac aaaatgtttc gcataagggt cacgaataat atatccgccc ttcatttttt
     3841 tctttaaaag taggaatgca ggattaatta tgaaatacat gaaacaattg aaagcggatt
     3901 tttttattgt gcaaaaaatt aatttcctat aaatggttta catattaagt tattaaacgg
     3961 ttttgatttg gataaaaatt aaatgtgttt aagcgagtat actcggttaa agacgggtaa
     4021 tggatgtttt tgtttggttt tttggggggg tgagaggttt gaatgtagga ttgtaattga
     4081 aagtgctggc ttcgaatatt aatgcacgat cgcggacagg agtccggggt ggcgtctgga
     4141 gttgaaaact ccacgacaac gcgaagtctt cgacttcttt tgtgtagggc gtagggttgg
     4201 tttggcgaat gtcaggtcat tgatataagc aaataacaaa tgaagtctta gacttcattt
     4261 gacgaattaa taattaaaat tctaatttag aattcccatg gatagcacta acccaccctt
     4321 tgtgtcgcag acacaaagtt gtcgtggagt tttcaactcc agacgccacc ccggtctccc
     4381 gaccggcgaa aaaaaatgtg gaggttaatc ggctttgtca ataattattt catcagcatc
     4441 ttccgattct atattcatag tcgcactcgt cacttgaaaa gttgtgcggc gattgagttg
     4501 atgcaaatac tcaaaattat ctttgttaga tttataggta ttaataaatg cttccgttaa
     4561 tacacttcct tttggaacaa ctttaccatc aggtaaaatt tcatctgtgg taagtgtgct
     4621 tggagaactt tcaccaaatc ctttaaatgt taatcgctca gcagcaatac ctttttcaat
     4681 taaatatttt acaacactat tggcacgacc gcgacttaat ttctgattgt atttgtctac
     4741 ccctcgggag tcggtatgcg aatttagttg aatatttaat cccggatttt catttaacaa
     4801 tttcaccagt ttatccagct cagggaatga ctctgcacgc aatgatgaat cgttgtaatc
     4861 gtaataaatg ttttttaatt taatttcacc tgctttttct ccactagcaa taggaatttt
     4921 atctaacact acagaggcat aaacaaaaac tgtatctccc ggttttcctt ttactgtttt
     4981 agtggtaaca taagtggtat tattaaaata accttctttc ataccggtta ttttgtaatc
     5041 ctttagtgaa tctacctgaa tgctgaaaat gccttcatct ccaatattcc atttccccaa
     5101 taaggtttgc tcattaggat aggagagggt tgctattgat ttatccaatg gaatagtatc
     5161 taatacggta ctatttacat caagactatc taaataaact ttttctaaaa cagttccttt
     5221 taaaacaaat accggaggtc gtaatttttt ggttgttttg gcgaaaaaat acatatcatc
     5281 atcacccatt ccacctttgc ggttagatga aaaataaccg gcacttaatg tatcagaatt
     5341 tgcaaattgt tttttatcca acataatcaa cccaaaatca tcagcacctg aattaatggg
     5401 atactccatg tttttaatat tgctgaattt attgtttttc caagttgcag aaaaaatatc
     5461 caaaccacct aatccgggat gtttgtcgct ggaaaaatac attacgccat cactgtcaaa
     5521 atatggaaac acttcattgc cattggtatt aatcacctgc cctgcattat atgctgcatc
     5581 ccatccactt tcggtgcgtc gactcatata aatatcggca ccaccgtatc catcaggatt
     5641 tgtagctgca aaaaataagg tattgccatc aggagataaa caaggtgttc caatattcag
     5701 ggtatcatca aaaaacggca gcgcaacagg ttcactccaa ccaccatccg gtaaataatt
     5761 gctcacataa ataccacaat aatcgtccac ctttctgtca tcagttccgc atcgtgtaaa
     5821 atacacttca gaataatctt tattaaatgc aagcgtaccg tcgttgtagg cagaattaaa
     5881 aggcccctta aataattccg gtgcagaaaa actagttaca tctttaatat tggcaac
//
LOCUS       DAORKW010000189         5916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_514_length_5916_cov_5.387647, whole genome shotgun sequence.
ACCESSION   DAORKW010000189 DAORKW010000000
VERSION     DAORKW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_514_length_5916_cov_5.387647"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="PLB46_08650"
     CDS             <1..676
                     /locus_tag="PLB46_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRF76625.1"
                     /translation="AAPAKPVAKAAKPVAKPVVKKQEKTKAPVAKPIKKATNTKAPVV
                     KNVTKNEVVKEKEVKKAVTKTVAPKPEPKKEAPVVKTPPRKFERQLKPKQAIVSLNTD
                     NRSRYSDAELAEFKELITGKLETSRGELKYLQEQISRSSEMGDDSDARFKGLEDGTST
                     SEREYLSQMASRQIQYIGHLEKALIRIENKTYGICRETGKLISKERLRAVPHATLSIE
                     AKQGKG"
     gene            651..1376
                     /locus_tag="PLB46_08655"
     CDS             651..1376
                     /locus_tag="PLB46_08655"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF011369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HRF76626.1"
                     /translation="MKQNKVKDNPVKKSYLAIGIILLVLLIDQIVKFWIKTTFALGEG
                     FDILGFSWAKIHFVENEGMAFGMELGGDYGKLALSLFRLIAVFFIGYYLYTLIKSKAG
                     NGLIISIALVFAGAMGNIIDSVFYGVIFSASTFGQVAEFMPEAGGYTTLLHGHVVDMF
                     YFPMAQGNYPDWMPFLGGKYFLFFRPVFNIADSAITIGVLSILIFQRSIFVNSDSDQK
                     PATENTTPVEIQEPLAEEEQPKT"
     gene            1436..2386
                     /locus_tag="PLB46_08660"
     CDS             1436..2386
                     /locus_tag="PLB46_08660"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HRF76627.1"
                     /translation="MKTIDDLQQFYQQEIVPGLSQLEVLRKAQLRRVRFMWIATVVSL
                     FVGFISMMPPLILVCLLISLLLYFYFFGFKRNRPDFKSEYKKVVIGKLIQFITPDLVF
                     TPNLFITQNKYSQSGIFLASPDIYKGEDLVSGKIDKTQVEFCELHTQDRQTDHKGNTH
                     YVTIFKGLFFIADFNKHFNGNTYVLSDFGERFLGGFGKMLQGLSIGRPDVVRLEDVEF
                     EKQFVVYSSDEVEARYILSTSFMEQIMAFKKKTNANIQLSFVGNNINVAIPMKENMFE
                     PSIRKTVLNFEDIKMYYSQLQFCTAIVDELNLNTRIWSKQ"
     gene            complement(2413..3324)
                     /locus_tag="PLB46_08665"
     CDS             complement(2413..3324)
                     /locus_tag="PLB46_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76628.1"
                     /translation="MEGISGKRILLVFAIVVFSLQTQAQCIIGNCENGYSVFKLENGD
                     MYTGQWVNGTREGYGRYDWASGAFYVGDFKYNGFHGLGTYYAPDGTVASGVFEDNVFQ
                     GADTTLVLPTPIAAPDILIWDEWIKADLTAKSAAAKGTKPVEFGAMVLKAISEFPTDF
                     ANIKTVDRPKILARETGWYSNLVTKESVEAGVGATSVSRKAMYYNILYTGSDSLAAIK
                     KYNNYIAVVKGLTIPCCSLIADTYDFSGPNYSSKTTSWLTLSVNGGFEESVYSDMVME
                     LSLNTMLGENGWQITYKIYHLSEMQNE"
     gene            complement(3345..3845)
                     /locus_tag="PLB46_08670"
     CDS             complement(3345..3845)
                     /locus_tag="PLB46_08670"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HRF76629.1"
                     /translation="MVIDQNEILQICLRVAVIYVIVLAGLRVMGKREIAQLSVIDFVL
                     VLLISNAVQNAMLGSNTTLFGGIVAAITLFVINFVLKQVENYGPFRKLLEGNPVLLIY
                     EGKVNASNLKKSGISMAELEAAVRAHGVEHIEEVNLGMLEVNGNISILSSNYTIKTET
                     KQIKQD"
     gene            3940..>5916
                     /locus_tag="PLB46_08675"
     CDS             3940..>5916
                     /locus_tag="PLB46_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRF76630.1"
                     /translation="MMLRNIVLYIFTGLLIQAAQAQESLLWKVTSPDGKYNSYVLAST
                     TLPGVENYDIASGTALIMDKVNTVAFFNVPDQSEIQNIPVFMKSSGDNTLKGYYKRED
                     RIRFELMVNDKLKTSPENYYGFKPLYVLQLFKEKDHAAGADFQQTILMDIALQQSKPT
                     LSLVTIRQIAGIMDQMDFSTQAMVLSSYVNNVQTYLDADAEKFNAYVQQSLTDYIKVV
                     NSAEQSAYINVMLNNMNDFLLKKIEPLSSQQSVLYVLDADLVAGESGILKKLKNKGFI
                     VTAEPLSIKLYAGNAPQLGDKNNNATASVTSNIPDDFPEMTLTPAINGYIDPETIKVI
                     NIKDNNSYNDGFLAYRDPFGDFFDMAAADTLFMESWYELKGTDANFKVKVPVKAEWEK
                     SETPWSNGGTIKKFIYSNNHAKSDLFYSVGYTVYPPNFVQNNKNNFFEQFIKSTQDQI
                     SGRIIDQRIVSNPNFTGREFTAVVGDSFFVRSQFLLQDNVLYQLLVGGPGDNPFSVYA
                     ESFLNSFQTATNVLVNWHFFEQPSFNCYLPTPPSKQTKTYPLAAGPLNVITYSSTDYK
                     ELVEYTVTASTYPPGHKFGNKNAFFEDLVATAEKSYIGKAYKTEKVENNGVEGRYIEM
                     QLMNSKIYRIYFYFDGNTVYQFAAGGDSQILVSNN"
BASE COUNT         1926 a         1080 c         1101 g         1809 t
ORIGIN      
        1 agcagctccg gcaaaaccgg ttgctaaagc cgcaaaaccc gtggcaaaac ccgttgtgaa
       61 aaaacaggaa aaaacgaaag caccggttgc aaaaccaatt aaaaaggcca ccaatacaaa
      121 agcccctgtg gttaaaaacg ttacaaaaaa tgaggttgta aaagaaaaag aagtgaaaaa
      181 ggcagtaaca aaaacagtgg cacctaaacc agagcctaaa aaagaggctc cggttgtaaa
      241 aacgcctccc agaaaatttg aaaggcaact aaagcctaag caggcgattg tttcgttaaa
      301 taccgataac cgttcacgtt acagtgatgc ggaattggct gaattcaaag aattaattac
      361 cggtaaactg gaaacatcac gcggtgagtt gaaatatctg caggaacaaa ttagccgcag
      421 cagcgaaatg ggtgatgatt cagatgcgcg ttttaaagga ttagaagatg gtacttctac
      481 ctctgaaaga gaatacctca gtcaaatggc ttctcgtcag attcaatata ttggccacct
      541 cgaaaaggcg ttaatccgta tcgaaaacaa aacttacggt atttgccgcg aaacaggtaa
      601 actgatttct aaagaaagat tacgcgctgt accacacgcc acattaagta ttgaagcaaa
      661 acaaggtaaa ggataatccg gtgaagaaat cctacctggc tatcggtatt attttgttgg
      721 tgttactcat cgaccaaatt gtaaaatttt ggattaaaac cacatttgca cttggcgaag
      781 ggtttgatat tctgggattt tcatgggcaa aaattcattt cgttgaaaat gaaggtatgg
      841 cctttggaat ggagcttggt ggcgattatg gcaaactcgc tttgagtttg ttcagactga
      901 tagcggtatt ttttattggg tactatttat atactttaat aaaatcaaaa gccggaaacg
      961 gcttaatcat aagcattgca ttggtatttg ccggcgcaat gggtaatatt atcgacagcg
     1021 ttttttatgg tgtaatattt tcggcctcca catttggtca ggtggctgaa tttatgcccg
     1081 aagccggagg atataccact ttattgcacg gacatgtagt agacatgttt tatttcccaa
     1141 tggcccaggg taactatccc gattggatgc catttttagg tgggaaatac tttttgtttt
     1201 tcagaccggt attcaatatt gcagatagcg ctattacaat tggcgtgctt tcgatactga
     1261 tttttcagcg cagtattttt gttaactccg attcggacca aaagccggca acggaaaata
     1321 ctactccggt tgaaatacag gaacctcttg cagaagaaga acaacctaaa acctgattat
     1381 tataagcgga ccgttcttaa ggtattttga cttaagtttg cggaacaatt agcagatgaa
     1441 aaccatcgac gacttacaac aattttatca gcaggaaata gtgccgggtt tatcgcaact
     1501 ggaagtattg cgcaaagcac agttacgcag agttcggttt atgtggattg ccaccgtggt
     1561 ttcacttttt gtgggattta tttctatgat gccaccacta attcttgtgt gtttactcat
     1621 ctcattatta ttatattttt atttttttgg atttaaacgc aatcgccctg attttaaatc
     1681 agagtataaa aaagtggtaa ttggaaaact tattcaattt ataacaccgg atttagtttt
     1741 tacacctaat ttatttataa cacaaaataa atactcacaa agcggcattt ttctggctag
     1801 tcccgatatt tataaagggg aagatttggt atccgggaaa attgataaaa cacaagtgga
     1861 attttgtgaa ttacatacgc aagaccgtca gaccgaccac aaaggaaata cccattatgt
     1921 gacaatattt aaaggccttt tttttattgc tgatttcaat aaacatttta acggaaatac
     1981 gtatgtgtta agcgattttg gtgaaagatt tttaggcgga tttggcaaaa tgctacaggg
     2041 tttaagcatt ggacgccccg acgttgttag gcttgaggat gttgagtttg aaaaacaatt
     2101 tgttgtttac agcagcgacg aagtggaagc cagatacatt ttatctactt cttttatgga
     2161 acaaataatg gcatttaaaa agaaaacaaa tgctaatatt caattgtcgt ttgtgggtaa
     2221 taatattaat gtggccatac caatgaagga aaatatgttt gaaccttcaa ttcgcaaaac
     2281 agtattaaat ttcgaagata ttaaaatgta ttattcgcaa ttacaatttt gcactgctat
     2341 agttgatgaa ttaaatttaa atacacggat ttggtccaaa caataattta attccgttaa
     2401 tatttttcta ctttattcgt tttgcatttc acttaagtgg taaattttat aagtaatttg
     2461 ccaaccattt tcaccaagca tagtattgag tgataattcc ataaccatat cgctatacac
     2521 cgattcttca aatccgccat ttaccgataa ggttaaccag ctcgtggttt tactgctata
     2581 attaggtccg gaaaaatcgt acgtatcagc aataagggaa caacaaggaa tggttaatcc
     2641 cttaacaaca gcaatgtaat tattgtattt ttttattgcg gctaatgagt cgctaccggt
     2701 gtacaaaatg ttataataca ttgcctttcg gctcactgat gttgctccta ctcctgcttc
     2761 taccgattct tttgtcacta aattgctgta ccaaccggtt tctctagcta aaatttttgg
     2821 tctgtcaaca gtttttatat tcgcaaagtc ggtgggaaat tcgctgatgg cctttaatac
     2881 catcgcccca aattcaacgg gttttgtccc ttttgcagcc gcagattttg ctgtgaggtc
     2941 ggcctttatc cattcatccc aaattaatat gtcaggcgcc gcaattggtg taggtaatac
     3001 caaagtggta tctgccccct gaaatacatt gtcttcaaac acacctgaag caacagtgcc
     3061 atcgggagca taataagtgc ccaaaccatg aaagccattg tatttaaagt cgccgacata
     3121 aaaggcccca ctggcccagt cgtacctgcc ataaccttca cgggtaccat ttacccactg
     3181 tccggtgtac atatcgccat tttcaagttt aaaaacactg taaccgtttt cgcaattgcc
     3241 aattatacat tgtgcttgcg tttgcaggct gaaaacaaca atggcaaata ctaacaaaat
     3301 tcttttacct gatatccctt ccataaatta ataacctgta atgcttaatc ttgttttatc
     3361 tgttttgttt cagttttgat ggtataatta cttgaaagaa tactgatgtt accatttacc
     3421 tccagcattc cgaggtttac ttcttcaata tgttccacac catgtgctct gactgcagct
     3481 tcaagttctg ccatcgaaat tcccgatttt ttcaaatttg aggcattcac tttgccctca
     3541 taaatgagta aaacggggtt tccttccaat aattttctaa atggaccata attttcgacc
     3601 tgttttaaca caaaattgat aacaaacaag gttatagcag caacaattcc gccaaaaagt
     3661 gtggtattgc ttccgagcat agcattttgc acagcattgc tgattaaaag caccaaaaca
     3721 aagtcgatta cgctgagttg agcaatctct cttttaccca taactctcag tccggcaagc
     3781 actatcacgt agataacagc aacccgaagg catatttgaa gtatttcatt ttggtcgatt
     3841 accatgtcac atcaattttg tgactgaaag gtagaaataa aaaactgttg ttaaaaccca
     3901 taactttacc ttaaatttac ccattcaaca gacaaatata tgatgctgag gaacatcgtt
     3961 ttatacattt ttacgggctt attgatacaa gctgcacagg cacaggaatc tttactctgg
     4021 aaagttacca gtcccgatgg caaatacaac tcatacgttt tggcttctac taccctgcct
     4081 ggcgtagaaa attacgacat tgcttccggc acagctttaa ttatggataa ggtaaatacg
     4141 gtagcatttt ttaatgtacc cgatcaatca gaaatccaga atattcctgt ttttatgaaa
     4201 tcttcaggag ataatacttt gaaaggttat tataaacgcg aagacagaat ccgttttgaa
     4261 ttaatggtga acgataaatt aaaaacgagt cctgaaaatt attacggatt taaaccatta
     4321 tatgttttgc aattatttaa agaaaaagat catgcggcag gcgctgattt tcagcaaacc
     4381 atattaatgg atattgcgct gcaacaatca aaaccgacat tgagtttagt taccatcaga
     4441 caaattgccg gtattatgga tcaaatggat tttagtacgc aggcaatggt gttaagcagt
     4501 tatgtaaata atgttcaaac ttaccttgat gctgatgctg aaaagtttaa tgcttatgtg
     4561 caacaatcat taaccgatta tattaaagtt gttaattcag ctgagcagtc agcttatatt
     4621 aatgtaatgc tgaataacat gaacgacttt ttacttaaaa aaattgaacc ccttagttca
     4681 caacaatcgg tattatatgt tttagatgct gatttggtgg ctggcgaatc ggggatatta
     4741 aaaaaattaa aaaacaaagg atttattgta acagcagagc ctttgagcat aaaattatat
     4801 gcaggaaatg caccacaatt aggcgataaa aacaataatg caacagcttc tgttacttcc
     4861 aatattcctg atgactttcc tgaaatgaca ttaacacctg caattaacgg gtatatagat
     4921 ccggaaacta ttaaagtcat aaatattaaa gataacaaca gttacaacga tggatttttg
     4981 gcctatagag atccatttgg cgattttttt gatatggctg ctgccgatac tttatttatg
     5041 gaaagctggt atgaattaaa agggactgac gctaatttta aagttaaagt tccagtgaaa
     5101 gcagagtggg aaaaatcaga aacaccatgg agtaatggcg gaactattaa gaaatttatt
     5161 tattcaaata accacgctaa atccgatttg ttttattcag ttggatatac ggtttacccg
     5221 ccaaattttg ttcagaataa taaaaataat tttttcgaac aatttataaa aagcacacaa
     5281 gaccaaatta gcggaagaat aatcgaccag cgtattgttt ctaacccgaa ctttacagga
     5341 agagaattta ctgcagttgt tggtgattcg ttttttgtgc gttcgcaatt tttgttacaa
     5401 gacaatgtgt tgtatcaatt attggttgga ggcccgggag ataatccatt tagtgtgtat
     5461 gccgaatcgt ttttaaattc atttcaaaca gcaaccaacg tattggtaaa ctggcacttt
     5521 tttgaacaac catcgtttaa ttgttattta cctacaccac cttctaaaca aacaaaaaca
     5581 tatcctttag cagcaggacc gttaaatgta attacttatt catctaccga ttacaaggaa
     5641 ttagttgaat ataccgtaac cgcaagcacc tatcctccag gtcacaaatt cggaaataaa
     5701 aatgcttttt ttgaggattt agtagcaacg gcagagaagt catatatcgg aaaagcctat
     5761 aaaaccgaaa aagtagaaaa taatggtgtt gaaggtcgtt atatagaaat gcaattgatg
     5821 aatagcaaaa tttatcgcat ctacttttat ttcgatggaa atactgtata ccagtttgca
     5881 gcaggaggcg atagccaaat cctggtgtcg aacaat
//
LOCUS       DAORKW010000190         5901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_517_length_5901_cov_4.581252, whole genome shotgun sequence.
ACCESSION   DAORKW010000190 DAORKW010000000
VERSION     DAORKW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_517_length_5901_cov_4.581252"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLB46_08680"
     CDS             complement(<1..269)
                     /locus_tag="PLB46_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRF76631.1"
                     /translation="MNLRLKQLNHVIIRIQHAFNNFRYLRWIKKMALCFAICLGLFFF
                     LNILFPVQVDIEYSQIVTARDGTVLNAFLTSDDKWRMMTEWSAITP"
     gene            complement(269..1432)
                     /locus_tag="PLB46_08685"
     CDS             complement(269..1432)
                     /locus_tag="PLB46_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76632.1"
                     /translation="MKAYLVSILLVFTTFLNPYVLSAQAMLGYTDAQGDFYVNDGGKI
                     IHLEPQRIISSQPAANSIAYISNSGNLTYYANHEQQKLEISNPSFYKNTDYYLYYATG
                     GSFSVYNGTARKYLGNIQQNPFAFGDSIAAMHDFSEYFYVYENDRFIELEQHPTKKII
                     AGDNIIAYVNHLDQFKIYFHGDKVDVDDYMPQKIAAGANIVAFIDSYNYLKVFYGGMI
                     HELFNVTQSTCLEIPYSIEDDGIENYCNAELVYDMQNALPLFQAGDDIVAYIDDMGQF
                     LVFDKGNIIELESQMPLHYEVVDNLLWYVDNNNYLKVYCNGELSIAETFYPEKIKADK
                     DVIVYTDLDKRLRAFYNNESIPVSANIVIDFSLNNTTIMYNEIPNKYKFHYLR"
     gene            1516..3093
                     /locus_tag="PLB46_08690"
     CDS             1516..3093
                     /locus_tag="PLB46_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76633.1"
                     /translation="MKRIIMLSIFTTLAAMFTRAQGVDVKTLPPIDELGTGTYMGKQG
                     GLYPNGSNTMPEAFYADAVLMAQSVQPLNSQGKPDPNGKIGLITIGASTVAMFSKGLE
                     NMVPNHKGVNKEISFVNCGIGGQDLSDIMEPSANFWKVIDGRLSESGLTFDQVQVIWF
                     QEDNLRNREDGFESRGKQLVDDFTYMVNFCKKRYPNLKLFYVTGRHTTEFMPPEGKDK
                     HREPKAYINGWACKWLIEKQIEGDKSLAYKGEDAVAPLILWGPYFWTQGDKPRTDGYT
                     WNKQLVSNDGVHPTDLGIERVSKDLVDFWSTDPVSQLWFLENPNDVSPVAITEESTTA
                     TVDMMQIQINNMMVHAILFDSIGERFRIAVVKDSVVVYSNEKAKRAKSFSVEIKDPGT
                     YKYVINDEFQHVYAGQFKIDENMQLMVITDENTTSASSIKPGKVIDPNAPAWVVNGTN
                     KLPKLKRVLNGHSNVKVVITDSKGKVVAEVADVLNQHTDLNELLERGEYGMQFFDVDG
                     TAIPLPEEFNSAVRIKY"
     gene            complement(3143..4936)
                     /locus_tag="PLB46_08695"
     CDS             complement(3143..4936)
                     /locus_tag="PLB46_08695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF76634.1"
                     /translation="MKRFSLIALLLSYLLILAFVAYYPKFNKPGTEATISYDVAGYYF
                     YLPAIFIYDDIHHLAFQEATNNKYQYTPGAYQGVQLSDGSYTMKYTIGAAIMYAPFFF
                     TAHIWANITGINADGFSPPYQQMISFGCLLYAFLGLWLLRKILLKYFSETATGLTIIS
                     LVLATNYLNYSAIDNAMTHNLLFTVFTGIIWFTIKFYEKPSFKYAIILGLLCGLATII
                     RPTDILICLIPIFWGVTNLTSLRERFVLLFKHGLKVLSFVAFAAVIGSIQLLYWKITT
                     GQFLFYSYENQTFDWTNPHITEGFFSYKKGWWLYTPFMVMIIPGFIYLYKNYKPLFLP
                     ILLFTVINIYIVFSWSIWWYGGSLGQRSLIESYALLSFPIAAVFQALTQAKKWIWALT
                     ACFVGFTGWYNIVLTIQAHNPAGILDAENTNRTYFWASFGRISIDNDIKRFLDTDEIY
                     KGDSNKSEVLYENNMEYDTLHVDTMHVINGKKCLFVDKNHQNTQPYRIPNPTTGSKWL
                     RVSATFFVTEKVWDVWKMPQFIIQFKNEDEVVKTKFIRVHRLMNSNQKRNLFFDVEIP
                     EKRYTTIDIFLWNADGMTATYMDDIRVEVLE"
     gene            complement(4954..>5901)
                     /locus_tag="PLB46_08700"
     CDS             complement(4954..>5901)
                     /locus_tag="PLB46_08700"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76635.1"
                     /translation="YKANLSVVPLDGKYLLAMSYLLAGDKKSYEKLLPSEFSGEESKQ
                     VFGGSFYSPIRDRAIALNCMLETDPENQQIGRLAQQLSEMMKNRYYLNTQERSFAFLA
                     FGKLARQSKDSNVTATIKANGTEVGKYTGKDLVIDNKSLINNNVEITTSGNGKLYYFW
                     TTKGVTADGSFKQEDNYLRVRKSFYNRWGTSIDLKNVKQNDLIVVKITLESATGTYVD
                     NVVITDILPAGFEIENDRLRATSSFDWIKNQSSAQHEDIRDDRINIYTTASSTLSTYY
                     YTVRAVSPGVFKMGPVMADAMYNEEYHSYNGAGIVKITR"
BASE COUNT         1958 a         1057 c         1033 g         1853 t
ORIGIN      
        1 ggcgttattg cagaccactc tgtcatcata cgccatttgt catcactggt taaaaatgca
       61 tttaatacgg taccatcacg agcggttaca atttgtgaat actcaatatc aacttgaaca
      121 gggaacaaaa tatttaagaa aaaaaacaac cctaaacata tcgcaaaaca taatgccatt
      181 ttttttatcc atcgcaaata acgaaaattg ttaaatgcgt gttgaatacg aataattaca
      241 tgatttaatt gcttaagacg taggttcatc aacgaagata atggaacttg tatttattag
      301 ggatttcatt atacataatg gtagtattgt tcaaactaaa atctatcaca atattagcag
      361 aaacagggat ggattcattg ttgtaaaatg cacgaagtcg tttatctaaa tcggtgtaaa
      421 caattacatc tttatctgcc ttaatttttt ccggataaaa tgtttctgct atacttaatt
      481 caccattaca atatactttt aggtagttgt tgttatctac ataccataac aaattgtcaa
      541 caacttcgta atgtaaaggc atttgcgatt ctaattcgat gatattccct ttatcgaaaa
      601 ctaaaaattg gcccatgtca tcaatatagg ctacaatatc atcaccggct tgaaataagg
      661 gtaaagcatt ttgcatatca tacaccaatt cagcattaca ataattttca atgccatcat
      721 cctctatact atagggaatt tcaaggcagg tggattgtgt aacattaaac aactcatgaa
      781 tcattccgcc gtaaaacact tttaagtaat tataactgtc gataaatgct acaatgtttg
      841 caccggcagc aattttttgt ggcatataat catctacatc aactttatca ccgtgaaagt
      901 atattttaaa ttgatcgaga tgatttacat aggcaataat attatcaccg gctataattt
      961 tttttgtagg atgttgctcc agctcaataa agcgatcatt ttcatacaca taaaaatatt
     1021 ctgaaaaatc gtgcatagct gctatactgt cgccaaacgc aaatgggttt tgctgtatgt
     1081 tccccaaata ttttcgtgca gtgccattgt aaacagaaaa gctgccgcct gtggcataat
     1141 ataaatagta gtcggtgttt ttataaaacg aaggatttga aatttcgagt ttttgttgct
     1201 cgtggttggc ataataggtt aagttaccgg aattggagat ataagcgatg ctgttggcag
     1261 ctggttgaga tgaaattatt cgttgaggct cgaggtgtat aatcttaccc ccatcgttga
     1321 cataaaaatc gccctgcgca tcggtatacc ctaacatagc ctgtgcggaa agcacatatg
     1381 ggtttaaaaa agttgtgaaa accagtaaaa tacttactaa atatgccttc attgctcagt
     1441 attgcttaaa aaaacgttca ttttctcatt aaaggtatga tatttgtaaa aatttgaatt
     1501 aaaaattccc acattatgaa acgaattatt atgctttcga tttttactac gctggcagct
     1561 atgtttacca gagcacaagg cgttgatgtt aaaactttgc caccaattga tgaacttggc
     1621 accggcactt acatgggtaa acagggaggc ctttacccca atggcagtaa cactatgcct
     1681 gaagcctttt atgcagatgc agtgcttatg gcacaatcgg tacaaccact caatagccaa
     1741 ggtaagcctg accctaacgg gaaaattggt cttattacta ttggtgcttc aacagtagcc
     1801 atgtttagca aaggtttgga aaatatggtg cctaatcata aaggtgtaaa caaggaaatc
     1861 agttttgtta attgcggtat aggaggacaa gacctaagtg atatcatgga gccatcagcc
     1921 aatttttgga aggttattga tggcagatta tctgaatcgg ggttaacttt tgaccaggtg
     1981 caggtaattt ggtttcagga ggataacttg cgcaatcgtg aagacggttt tgaaagcagg
     2041 ggaaaacaac tggttgatga ttttacctac atggtaaatt tctgcaaaaa acgttatcct
     2101 aatttaaaat tattttatgt gaccggacgc catacaaccg aatttatgcc gccggaagga
     2161 aaagacaaac accgcgagcc aaaagcatat attaatggtt gggcttgtaa atggttaata
     2221 gaaaaacaaa ttgaaggaga taaatcactt gcttataaag gcgaagatgc tgttgcacca
     2281 ttaattttat ggggacctta tttttggacc cagggtgata aacctcgcac ggatggttat
     2341 acctggaaca aacaattggt ttccaacgat ggcgtgcatc caactgattt aggtattgaa
     2401 cgtgtctcaa aagatttagt agatttttgg agcacagacc ctgtttcaca gttgtggttt
     2461 cttgaaaatc caaatgatgt atcgcctgta gcaattactg aggaatctac tactgcaact
     2521 gtagatatga tgcaaattca aattaataat atgatggttc acgccatttt atttgatagc
     2581 ataggagaac gttttcgcat agcggtagtg aaagattcgg ttgtagttta cagtaatgaa
     2641 aaagcaaaac gtgcaaaaag tttttcagtt gaaataaaag acccaggaac ttataaatat
     2701 gtaataaacg atgagtttca acatgtttat gctggacagt ttaaaattga tgaaaatatg
     2761 cagttgatgg taattacaga tgaaaatacc acttccgcgt cttcaatcaa gcctggtaaa
     2821 gttattgacc caaatgcacc ggcctgggta gttaatggca ccaacaaatt accgaaatta
     2881 aaacgcgtat taaatggcca tagcaacgta aaagttgtta ttaccgattc aaaagggaaa
     2941 gtagttgctg aggtagcaga tgtattaaat cagcataccg atttaaatga attattggaa
     3001 agaggggagt atggaatgca gtttttcgat gttgatggca ctgccattcc actgccggaa
     3061 gaatttaata gtgcagtaag aattaaatat taatagttac caaaatattt tgagcgggat
     3121 gtatttttat atcccgcttt ttttattcta gcacttcaac acgaatgtca tccatataag
     3181 tagcagtcat cccatcggca ttccataaga atatgtcaat tgtagtgtat cttttttccg
     3241 gaatttcaac atcaaaaaat aaattacgtt tttgattgct attcattaat cggtgaacgc
     3301 gaataaattt tgtttttacc acttcatctt catttttaaa ttggatgata aattggggca
     3361 ttttccaaac atcccacact ttttcagtaa caaaaaatgt agcagaaaca cgcaaccact
     3421 tactaccggt agtagggttt ggaattctgt aaggttgagt gttttgatga tttttatcta
     3481 caaacaaaca ttttttacca tttattacat gcatggtatc aacatgtaag gtgtcatatt
     3541 ccatattgtt ttcatacaat acttcacttt tgttgctatc acctttgtaa atttcatctg
     3601 tatctaaaaa acgctttata tcattgtcaa tagatattct accgaatgaa gcccaaaaat
     3661 aggtacggtt agtgttttct gcatctaaaa tacccgcagg attatgtgcc tgaatagtca
     3721 gcacaatatt ataccagcct gtaaatccca cgaaacatgc tgttaatgcc catatccatt
     3781 ttttagcctg tgtcaatgcc tgaaataccg ctgcaattgg aaaacttaat agtgcatatg
     3841 attcaattaa actcctttgt cccaaactac caccatacca ccaaatagac caactaaaaa
     3901 caatatagat attgataaca gtaaagagca aaatgggtaa aaaaagcggc ttataatttt
     3961 tatataaata gataaatccc ggtataatca ttaccatgaa tggagtatat aaccaccatc
     4021 cttttttata agaaaaaaaa ccttcagtga tatgtggatt tgtccagtca aatgtctggt
     4081 tttcgtaact ataaaaaagg aattggcctg tggttatttt ccaatacaat aactgtatgc
     4141 tgccaattac tgctgcaaag gcaacaaaac ttaatacttt taagccgtgt ttaaataata
     4201 aaacaaatct ttcacgtaat gatgtaaggt tggttactcc ccaaaaaatc ggaattaaac
     4261 aaattaatat atccgttggt cgtataatag tcgccaagcc acataataag cctagtatga
     4321 tggcatattt aaaacttggt ttttcgtaaa atttgatggt aaaccaaata atgccggtaa
     4381 aaaccgtgaa caataaatta tgtgtcatgg cattatctat cgcactataa ttaaggtaat
     4441 tagtagccaa aacaagtgaa atgatagtca gcccggtcgc agtctcagaa aaatatttca
     4501 gcagtatttt tcgtaataac cacaagccta aaaaagcata taataaacac ccaaagctta
     4561 tcatctgttg ataggggggt gaaaatccat cggcattaat tcctgtaata ttggcccaga
     4621 tatgcgctgt gaaaaaaaag ggtgcgtaca taatcgcagc acctatggtg tacttcatcg
     4681 tataacttcc atcagataat tgaacaccct ggtaagcacc cggcgtatat tgatatttgt
     4741 tatttgttgc ttcctgaaat gctaagtgat gaatatcatc ataaataaaa atagcaggca
     4801 gataaaagta ataccccgcc acatcataag aaatggtagc ttccgttccc ggtttgttga
     4861 acttagggta ataagcaaca aatgctaaaa tgagtaaata acttagcagt aatgcgatta
     4921 atgaaaatcg tttcattact gctaagttaa attttatctt gtaattttta caataccggc
     4981 gccattataa ctatgatact cttcattata catcgcatcg gccattaccg gccccatttt
     5041 aaatacaccc ggtgaaactg cccttacggt ataataataa gtgcttaatg ttgaactggc
     5101 agtggtataa atattaatcc tgtcatcacg aatatcttca tgttgtgctg aactttgatt
     5161 tttaatccag tcgaaacttg aagtggctct taaacggtca ttttctattt caaaccctgc
     5221 aggtaaaata tccgtaatca caacattatc tacataagta ccggttgcac tttctaaagt
     5281 tatcttcacc actattaaat cgttttgttt cacatttttt aaatcaatag aagtccccca
     5341 acgattataa aacgatttgc gaacacgtaa ataattatct tcctgcttaa acgaaccatc
     5401 ggcagtaaca ccttttgttg tccagaaata atataattta ccgtttccac ttgtggtaat
     5461 ctcaacatta ttattgatga gcgatttatt gtctattacc aaatctttac ccgtgtattt
     5521 tccaacttcg gtaccattag cttttatagt tgcagttaca ttcgaatctt tactttgacg
     5581 tgcgagttta ccaaacgcta aaaacgcaaa tgaacgttcc tgtgtattta ggtaataccg
     5641 gtttttcatc atctcagaca gttgttgagc taaacgacca atttgttgat tttcaggatc
     5701 ggtttccaac atacagttta aggctatggc tctgtcgcgg ataggagagt agaaactacc
     5761 gccaaatact tgtttcgact cttcgccact aaactcagaa ggcaataatt tttcataact
     5821 ctttttatct cctgctaata aatatgacat ggccaataaa tatttaccat ctaaaggcac
     5881 cacactcaaa ttggctttgt a
//
LOCUS       DAORKW010000191         5893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_521_length_5893_cov_4.235868, whole genome shotgun sequence.
ACCESSION   DAORKW010000191 DAORKW010000000
VERSION     DAORKW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_521_length_5893_cov_4.235868"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PLB46_08705"
     CDS             <1..659
                     /locus_tag="PLB46_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76636.1"
                     /translation="LKANYDGHEILFKHALYSTDFLKQAKPEEKTYKVISGLRNPMDI
                     CVSNYFKFKTDHENRYSNPRLMQHGFLRKYIMRWWNVRQYKSIVEKGETFEQFFTRAY
                     SVPYASWSIIEHKRMDFIIRFENLNDDFAQALKVIGVDKVRDIPVANKTAEKTKTFWE
                     YYETDTAKRRAKYIFGPYLKRWNYDFPESWNHIQVPWYSFFMYRMVNIARVIFWKFLR
                     "
     gene            660..1610
                     /locus_tag="PLB46_08710"
     CDS             660..1610
                     /locus_tag="PLB46_08710"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRF76637.1"
                     /translation="MKQTLPTFLIVGAVKAGTTSLHEYLQMHPEVYMSPVKETNHFSD
                     GDMLFEHFNVDYKQDVNVNLEKYLEGPMDKKIHIAHVRTFQQYLQLYRNVTNQKAIGE
                     VSNSYLYLPNTATTIKQVLPDVKIVMILRNPIERLYSQYLMNLKLGKIIERDLLKEIA
                     SDQAKPIKGWGVSHLYLEVGNYYEQVKRYYDNFPAQNIKVILFDDFKKDAAGTMKDLF
                     HFLGVDENFALDMSQRYNEAGMPRFGKLNYWLTQIGVYGMVKKIFSPELKEKIKSIIY
                     TKDNIPKITPPEKTHLQNYYRNDIEALAKLLNRDLSAWLQ"
     gene            complement(1613..3007)
                     /locus_tag="PLB46_08715"
     CDS             complement(1613..3007)
                     /locus_tag="PLB46_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRF76638.1"
                     /translation="MKPFGKNLSERALRWIRWHEKLINFTQKHLNDKQFVIISGVLIG
                     ITSALAAILLKGMVFYINGFLFNQVSTGHISEYLYALFPMVGIVATAIIVKYRFRGNL
                     GGGNSAIIYAIKRKESKMPFHLMYAHIFTSAITVGTGGSTGLETPIVTTGAAIGSNYG
                     KTYKVKPEERTIMLACGVAAGIAATFNAPIAGVLFAIEVLISEISVVAFIPILIAAAS
                     GAILSDIILNENVLLSFDLLEPFNYRNVPFYVLLGLVAGVVSVYYIRMFIVIESLLKR
                     VKQKYYLHKAIIGGLLLGGLILLFPPLFGEGYIAIKNIADLDPGSSLAGSILDGNYND
                     PWALILFSVIIMLLKVIATGITLGSGGNGGNFAPSLFTGAYLGYVFASIINLTGLVNV
                     PVSNFTIVAMAGILSGIFHAPLTAIFLIAEVTGGYDLIVPLMIVSALSFAVSKYLFEY
                     PLDGVKLRKMLLQQ"
     gene            3142..3720
                     /gene="rpsP"
                     /locus_tag="PLB46_08720"
     CDS             3142..3720
                     /gene="rpsP"
                     /locus_tag="PLB46_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRF76639.1"
                     /translation="MATKIRLQRHGRKKAPFYHIVVANVTSPRDGKFIERLGTYNPTT
                     VPAQITIDVNKAVEWLQKGAQPTTTAAAILRYKGVLYKKHLLRGVSKGSFTLEVAEQK
                     FVEWQQSHEGAVMDHVKKVEAGEAAKKAAEIARINNKRLEEASKKAAAEKEAADAAAA
                     ANATVEATEEPTAEVETEVTDNAATTEETTNE"
     gene            3725..4231
                     /locus_tag="PLB46_08725"
     CDS             3725..4231
                     /locus_tag="PLB46_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76640.1"
                     /translation="MSNELVQIGYVIKTHGVQGHLRIAFDENIKELSEQEALYFLVKG
                     NQIPFFIKTISYFKNGDALVLLEEITNKEDAERFTKKAVSGPDTYVEEEEEEYEDPWV
                     DFIVTDEVIGLLGKVNGSIDMAEYILLEIDYLGKTIMIPVHDEVIVALDESKKELITR
                     LPAGILDI"
     gene            4245..4925
                     /gene="trmD"
                     /locus_tag="PLB46_08730"
     CDS             4245..4925
                     /gene="trmD"
                     /locus_tag="PLB46_08730"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRF76641.1"
                     /translation="MRIDIITVLPELLVSPFALSILKRAREKGLLEVNVVNLRDYSTF
                     NHKQVDDYQFGGGAGMVMMVEPIFNCIQALKKDRTVDEVIYLTPDGERFNQKTANQLS
                     LKNDLILLCGHYKGVDERVRQHLITREISIGDYVLSGGELAAAVLVDALGRLLPGVLN
                     DETSALFDSFQDDLLAPPVYTRPASFNGWEVPDVLLSGHAAKIEDWRHEQALTKTRER
                     RPDLLHEN"
     gene            4991..5350
                     /gene="rplS"
                     /locus_tag="PLB46_08735"
     CDS             4991..5350
                     /gene="rplS"
                     /locus_tag="PLB46_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRF76642.1"
                     /translation="MDFIKSLELDLIPGKEIPQFKAGDTITVNYKIIEGDKSRIQAFK
                     GDVIQRKGQGRTQTFTVRKISNGVGVERIFPLFSPNIDSIEVNKIGKVRRAKIFYLRG
                     LKGKSARIKEAKSAVTQ"
     gene            complement(5412..>5893)
                     /locus_tag="PLB46_08740"
     CDS             complement(5412..>5893)
                     /locus_tag="PLB46_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HRF76643.1"
                     /translation="HNTDSLMLVIRAIRKYQPDYVFCNAPEDRHPDHGKASKLVEEAA
                     FLSGLVKITTEVDGINQQAWRPKMVLKYIQDRWLTPDFVVDITPYWEKRMESIKAHKS
                     QFFDPNSNEPQTYIASKNFFDGIEARAREMGRSAQFQYGEGFITSRIPGVKNITELY"
BASE COUNT         1948 a         1077 c         1147 g         1721 t
ORIGIN      
        1 aattaaaagc taattatgat ggccatgaaa ttttatttaa acatgccttg tatagcaccg
       61 actttttaaa acaggcaaaa cctgaagaaa aaacatataa ggtaattagt ggtttgcgca
      121 atcctatgga tatttgtgta agtaattatt ttaaatttaa aacagaccac gaaaacagat
      181 attcgaatcc tcgtttaatg caacatggct ttttgcgtaa atacattatg cgttggtgga
      241 atgttcgtca gtataaatca attgtagaaa aaggcgaaac ctttgaacaa ttttttacac
      301 gtgcttacag cgttccttac gcaagctgga gtattattga acacaagcga atggacttca
      361 ttattcgttt cgaaaatctg aatgatgatt ttgcacaggc cttaaaggta attggagtag
      421 ataaagtaag agatattccg gtagccaata aaactgctga aaaaactaaa acattttggg
      481 agtattacga aactgatact gcaaaacgca gagcgaaata tattttcgga ccatatctaa
      541 aacgctggaa ttatgatttc cctgaaagct ggaatcatat tcaggtgcct tggtattcat
      601 tttttatgta tcgtatggta aatattgcca gagtaatatt ttggaaattt ttgcgctaaa
      661 tgaagcaaac ccttcccaca tttttaattg taggtgctgt taaagccgga actacttcac
      721 tgcatgagta tttacaaatg catccggaag tgtatatgtc gcctgtaaag gaaacaaatc
      781 actttagtga tggtgatatg ttgtttgagc attttaatgt ggattacaaa caagatgtaa
      841 atgtgaattt agaaaaatat ctggaaggcc caatggataa aaaaattcat attgcgcatg
      901 tccggacttt ccaacaatac cttcagttat atcgtaatgt taccaaccag aaggcaatag
      961 gtgaagtaag taattcgtat ctatatctac ccaatacagc aactaccatt aaacaagtgt
     1021 tgcctgatgt aaaaatcgtg atgattttgc gcaatcctat tgaaagattg tattctcaat
     1081 atttgatgaa tcttaaactg ggaaaaatta tcgaacgcga tttattaaaa gaaatagctt
     1141 ccgaccaggc taaaccgatt aaaggttggg gagttagtca tttatatttg gaagtaggta
     1201 attattatga gcaggttaaa cgatattatg ataatttccc tgctcaaaat attaaagtga
     1261 ttttgtttga tgactttaaa aaagatgcgg caggtacaat gaaggatttg tttcatttcc
     1321 tcggtgtaga tgaaaatttt gcacttgata tgagtcagcg ttacaatgaa gccggtatgc
     1381 cacggtttgg caaactaaat tattggctta cacaaattgg ggtttatggt atggtgaaaa
     1441 aaatattttc gcctgaactg aaagaaaaaa ttaaaagtat tatctataca aaagataata
     1501 tcccaaaaat tacgccgccg gaaaaaacac atttacaaaa ttattatcgc aacgatattg
     1561 aggcattagc gaaattactc aatcgcgatt tgtcagcctg gttacaataa tattattgtt
     1621 gtaataacat cttccgcaat ttaacaccat cgagtggata ctcaaataaa tatttggaga
     1681 ctgcaaaact aagtgcagat acaatcataa gcggaacaat taaatcataa cctcccgtta
     1741 cttctgcaat taaaaatata gcggtaagcg gagcatgaaa tatcccactc aaaatacccg
     1801 ccattgctac aattgtaaaa ttacttaccg gcacatttac taatccggtc aggttaatta
     1861 ttgaagcaaa aacatatcct aaataagcac ctgtaaataa tgatggtgca aaatttccac
     1921 cattgcctcc acttcctaat gtaattcctg tagcaattac ttttaacaac atgataatca
     1981 ccgaaaataa aattaatgcc catgggtcat tataattacc atctaaaata cttcccgcca
     2041 gtgatgagcc cgggtcaagg tccgcaatgt ttttaattgc aatgtatcct tcaccaaaca
     2101 gcggtggaaa taataagatt aatccgccta acaataagcc acctataatt gctttgtgca
     2161 ggtaatattt ttgtttaacc cgttttagta gagattcaat tacaataaac atacggatat
     2221 aatacactga aacaactccc gcaacaagtc cgagtaaaac ataaaagggt acatttctat
     2281 aattaaacgg ctccaataaa tcgaagctta acaacacatt ttcatttaaa ataatatcag
     2341 ataatatagc gcctgaagct gcagcaatta aaataggaat aaacgcgact accgatattt
     2401 cactgattaa tacttcgatg gcaaataata ctcccgcaat tggtgcatta aaggtagctg
     2461 ctataccagc tgccacaccg catgccaaca taatagttct ttcctccggt ttcactttat
     2521 acgttttgcc gtagttagaa ccaattgctg cacctgtagt aacaatcggt gtttccaatc
     2581 cggtagaccc accggtgcca actgtaattg cactggtaaa aatatgggca tacatgaggt
     2641 gaaagggcat tttgctctcc ttgcgtttta tcgcataaat tatggcacta ttgcctcctc
     2701 caaggttacc ccggaagcga tattttacaa taatggcagt ggcaactata cctaccattg
     2761 gaaacaaagc atataaatat tctgaaatat ggccggtgct cacctggtta aacaaaaatc
     2821 cattgatata aaacaccatg ccttttagca aaatggcagc cagggcagaa gtaataccaa
     2881 tcaaaacccc tgaaataatg acaaattgct tgtcgttcag gtgtttttgg gtgaaattta
     2941 tcagcttttc atgccaacgt atccaccgta aagcgcgttc tgataagttt tttccaaatg
     3001 gtttcatagt ttgtaagggg ctaaagtaag caaatgttat ggtaaatcat gaagaatttg
     3061 tatctttgca ccccaatctc aaacctcccg ttaatcggga ttgattctga gacattttta
     3121 actaaaaaaa catgttttaa catggcaact aaaatcagat tacaacgtca cggtagaaaa
     3181 aaagcgcctt tctatcatat cgtggtagca aatgttacct caccacgcga cggtaaattt
     3241 attgagcgcc ttggaactta caacccaaca actgttcctg cacaaatcac aatcgatgta
     3301 aacaaagcgg tagaatggct tcaaaaagga gctcaaccta cgacaaccgc tgcggcaatt
     3361 cttcgctaca aaggtgtttt atacaaaaaa cacttattac gtggtgttag caaaggctca
     3421 tttacattag aagttgcaga acaaaaattt gttgaatggc aacaatctca cgaaggtgca
     3481 gttatggacc acgtgaaaaa agttgaagcc ggtgaagctg ctaaaaaagc tgctgaaatt
     3541 gcacgtatca ataacaaacg tttagaagaa gcatctaaaa aagctgctgc tgaaaaagaa
     3601 gctgctgatg ccgctgctgc tgcaaatgct actgtagaag caactgaaga acctactgca
     3661 gaagtagaaa ctgaagttac agataacgca gctacaactg aggaaactac aaacgaataa
     3721 tatcgtgagc aacgaactgg ttcaaatcgg ttacgttatt aaaactcacg gtgttcaagg
     3781 acacctcaga attgcgtttg atgaaaatat taaagagctt tccgaacagg aagctcttta
     3841 ttttttggta aaaggcaacc aaattccatt ttttattaaa actatcagtt atttcaaaaa
     3901 tggtgatgcc ctcgttttac tggaggaaat taccaataaa gaagatgctg aacgatttac
     3961 caaaaaagcg gtttcaggtc ccgatactta tgttgaagaa gaagaggagg agtatgagga
     4021 tccatgggtt gattttattg ttaccgacga agtgattggt ttgttgggca aggtaaatgg
     4081 ctcaattgat atggctgagt atattttgct ggaaattgac taccttggaa aaaccatcat
     4141 gattccggtg catgatgaag ttatagttgc acttgacgaa tcgaaaaaag aattgataac
     4201 ccgcttaccg gcaggaattc tggacattta accaatagta tcacatgcgt atcgacatca
     4261 ttactgtttt acccgaattg ctggtttcgc catttgcact cagtatcctg aaacgcgctc
     4321 gtgaaaaggg tttgctggag gttaatgtgg tgaatttgcg cgattattcc acgtttaatc
     4381 acaaacaggt ggacgactac caatttggtg ggggagcagg tatggtgatg atggttgaac
     4441 ccatttttaa ttgtattcag gcgctgaaaa aggacaggac tgttgacgaa gtcatttacc
     4501 tgacacctga tggtgaacga tttaaccaga aaacagccaa ccaactttcc ttaaaaaacg
     4561 acctgatttt attgtgtggc cattataaag gggtagatga aagggtgcgt cagcatctga
     4621 taacccgcga aatttctatt ggcgattatg tgctgagtgg gggagaactg gcggctgcgg
     4681 ttttggtgga tgccttgggg cgactgcttc ccggggtgtt gaatgatgaa acttcggctt
     4741 tatttgacag ttttcaagac gatttactgg caccgcctgt atataccaga ccggcatcgt
     4801 ttaatggttg ggaggttcct gatgtgcttt taagcggaca tgccgccaaa attgaggatt
     4861 ggcgccatga acaagcctta actaaaaccc gtgaaagacg ccctgattta ctgcatgaaa
     4921 actgatacag aaagtttgta ttacaacgaa tttctcctat ctttgcagtc cacttttaaa
     4981 aaataaggtt atggacttta taaaatcgct agaattagac ctgattccag gtaaagaaat
     5041 ccctcaattt aaagcaggtg atactatcac tgttaattat aaaattattg aaggggacaa
     5101 atcaaggatc caggcattta aaggcgacgt aatacagcgc aaaggtcagg gtagaactca
     5161 aacttttact gttcgtaaaa tttctaatgg cgttggggta gaaagaattt tcccattatt
     5221 ttcgccaaac atcgatagca ttgaagtaaa caaaattggt aaagttagaa gagctaaaat
     5281 cttctatctt cgtggtttaa aaggtaaatc agcaaggatt aaagaagcta aatctgctgt
     5341 tacccaataa gatagtctgt cgtgattata tgtatatcag gatcccggtt cgtaaggact
     5401 gggattttta tttaatataa ttccgtaata tttttcacac ccggaatacg gctggtaata
     5461 aatccttcac catactgaaa ctgtgcactg cgacccattt cacgggcacg cgcttcaata
     5521 ccgtcgaaaa agtttttgga tgctatatac gtttgaggct cattgctgtt tgggtcgaaa
     5581 aattgcgatt tatgtgcttt tatactctcc attctttttt cccagtaagg tgtaatatcc
     5641 accacaaaat caggtgttaa ccaacggtcc tgaatatatt tcaaaaccat ttttggtcgc
     5701 caggcttgtt ggtttatacc atccacttca gtggtaattt ttactaaacc agataaaaat
     5761 gctgcttctt caactaattt ggaagctttg ccatggtcgg gatgtctgtc ttccggagca
     5821 ttacaaaaaa cgtaatcagg ttgatatttc ctgatggccc tgattaccag cattaatgaa
     5881 tcggtattgt gct
//
LOCUS       DAORKW010000192         5877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_523_length_5877_cov_5.776194, whole genome shotgun sequence.
ACCESSION   DAORKW010000192 DAORKW010000000
VERSION     DAORKW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_523_length_5877_cov_5.776194"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PLB46_08745"
     CDS             <1..972
                     /locus_tag="PLB46_08745"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRF76644.1"
                     /translation="AIFFYAILIFEHLAQEAYRTFVVFSKPIIANIILFLRTGSWAYL
                     ILILWTAGVDELKSLRWLYLFWMCGGIAALAVSLYFLAKFKFKSVKHIPIDWKWIKQG
                     IKVSLLFFIGTVGYKIIEFADRYFIDYYHTKEEVGVYTFYANMCNIVETFVHTAVIII
                     FSPRLIETFHKSNYDYRKTLAQFAKQVVIYTIIIGVALAILIIPLLKSLENDEYIRDY
                     NAFVVLVLSKMVLNFSLIFHYILYVRKNDFPIIKATIIACVINILLNFILIPSMSIMG
                     AALATLTSFLIVMLMKMYYTRNLPEAKQIIYLRFLRQRRSKKVNNDN"
     gene            984..2195
                     /locus_tag="PLB46_08750"
     CDS             984..2195
                     /locus_tag="PLB46_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76645.1"
                     /translation="MKTYFENIEWYDYLIFLVYNGVFSYLIYRLGDVFFNISKKATLV
                     LVLIFQFYFLLVVGDSFFNYLPYLPDSELYSFMISSGQYPETSSENILVLYYATLFIG
                     TICLNSPVIFVFFCIFLYLIALMIFIRAWKVALPTFNAAGEFTATLLMLVWPASMVYM
                     TAPLREAFILFGFALFFNGFINFLYKQEWKSLLIGSLIICAIRLQLIVMVFPVLGALA
                     VYKLKINRLAKVSIMVSTLVIALLAVRYILVEEPLSPETMADLRNRGILLVGELGYGN
                     VYWDSYLDMARDYPFIILQFLFSPLPIFVQHNPIDTLIPFLDCIFLIFLFIVIFPKLR
                     LHIKNHLPLLLFLLFFIILFGGYEYHLGGAVRHRMPLVIILILLSSQTISNFLFKRKP
                     DEETEHPAVNH"
     gene            2161..2973
                     /locus_tag="PLB46_08755"
     CDS             2161..2973
                     /locus_tag="PLB46_08755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF76646.1"
                     /translation="MKKLSILLSTINDRIYAAEPLLQNRFEDTEFVVVHQITNLSKTD
                     TYSNFYNKFDSAYVRFVPMFDKGTGKSRNLAMQKAEGELMYLCDDDLVFQPDFYTNIM
                     SAAAAFPDIDIFTFQVLTTDGLPFKQYPEQAYQHTVRTTAKVSIVEMVIRKQAYATGK
                     IKFDERFGLATIYNTGEEFVMLSDALKKGITAKFIPQYLVSHPPISSGKIFNEEVAFD
                     KGAMTARVYGWKFIFANAIFALKKYTTYRKSIGFFSFTYHMYKGSLHFLKND"
     gene            2966..3721
                     /locus_tag="PLB46_08760"
     CDS             2966..3721
                     /locus_tag="PLB46_08760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF76647.1"
                     /translation="MTNPLVSIIMPVYNSEKVLKAAIDSVLQQTYANWELIIVDDKSS
                     DQSVTIIQTYAAQNTNIILIEQAVNSGAAITRNKGISIAKGKYIAFLDADDIWLPEKL
                     ALQIAFMEKNQSALTCTYYSTMQQDSTPVKSIIKAPDRITYHHLLKNNTIGCLTAVYN
                     VEICGKQYMPEIRKRQDYGLWLNILRAGFVAETIPLVLAQYRIGADSLSQNKWKVLGY
                     NWELLRKHQQLSVVKSLYYFTCFLWNKTFKYLK"
     gene            3718..4386
                     /locus_tag="PLB46_08765"
     CDS             3718..4386
                     /locus_tag="PLB46_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRF76648.1"
                     /translation="MNNRAIILGASGLTGSTLLSLLCEQEYYAEVICFVRKQIHNIHD
                     KQQNILTDFNDLESHAALFQHADVYCCLGTTNKNVNNDKEAYREADLYRPMRIAKLLK
                     ANNGKQLLIISAMGADESSRIFYNKLKGELQRKLIEMQLPCLHIFQPSLLLGDRREHR
                     SGEKFATALMAFIQPMLIGGWRKYRAIHVKAVAKAMFIKAKQSGSGNHIYTSDIIQQI
                     ADAG"
     gene            4376..5305
                     /gene="queG"
                     /locus_tag="PLB46_08770"
     CDS             4376..5305
                     /gene="queG"
                     /locus_tag="PLB46_08770"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRF76649.1"
                     /translation="MQDNVKSHITQFIKTAAANAGFSFCGIAKAEQLPDEARKLERWL
                     NQGKHGKMGYMENYFDKRIDPTQLMPGAKSVISLLYNYFPEQTLSSSLKIAKYAYGED
                     YHDVIKEKLRLLIAELQHEIGDFEARIFVDSAPVLEKAWAQKSGLGWQGKNTNLINPK
                     LGSFYFLAEIICDLPLHYDHPIKDYCGTCTACVDACPTDALVEPYQIDASKCISYLTI
                     ELKDALIPEQFHGKMENWIFGCDICQDVCPWNRFAKQHQEPKFFPTDDLKNVAANDWK
                     EVTAEIFNQVFKHSAIKRTKWSGWERNIITAKK"
     gene            complement(5306..5623)
                     /gene="miaE"
                     /locus_tag="PLB46_08775"
     CDS             complement(5306..5623)
                     /gene="miaE"
                     /locus_tag="PLB46_08775"
                     /inference="COORDINATES: protein motif:HMM:NF017939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HRF76650.1"
                     /translation="MKELIKFQKKGGSREDQLLEKLLMAALIEARSCERFRLLSEGLA
                     DEEMRNFYHEFMVSEAGHYKMFISLANNYINPDKVKKRWQEWLEFEASVMKNMQLSGE
                     RIH"
     gene            complement(5648..>5877)
                     /gene="miaE"
                     /locus_tag="PLB46_08780"
     CDS             complement(5648..>5877)
                     /gene="miaE"
                     /locus_tag="PLB46_08780"
                     /inference="COORDINATES: protein motif:HMM:NF017939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HRF76651.1"
                     /translation="KLPTDPRWVNLAEKSFEEILTDHAYCEQKAASTCISLIQLFPDR
                     ERLVDEVSPVVTEEWGHFRMVLKELKKRDLK"
BASE COUNT         1790 a         1051 c         1106 g         1930 t
ORIGIN      
        1 gccatcttct tttatgcaat tttaatattt gaacatttag cgcaggaagc atatcgtacg
       61 tttgtagtgt tttcaaaacc tattattgcg aatatcattt tatttcttag aacaggttcc
      121 tgggcttatc tgattttaat tttatggaca gccggagtgg atgaattaaa aagtttgcgc
      181 tggttgtatt tgttctggat gtgcggagga atagcggcgc tggcggtttc cctgtatttt
      241 ttagccaaat tcaaatttaa atctgtcaaa catattccta tcgactggaa atggattaaa
      301 cagggtatta aagtgagttt gctgtttttt attggtactg tcgggtataa aattatagaa
      361 ttcgccgatc gttattttat cgattattat cacactaaag aagaagtagg ggtttatact
      421 ttttatgcca acatgtgtaa tatcgtagaa acatttgtgc atactgcagt tattattatt
      481 ttttctccgc gactaattga aacgtttcat aagagtaatt atgattatcg taaaacctta
      541 gcgcaatttg cgaaacaggt ggtaatatac accatcataa tcggcgtggc tttggccata
      601 ttgataatcc cgttattaaa atcgcttgaa aacgatgaat acattcgcga ttacaacgca
      661 tttgtagtgc tggtattatc caaaatggtt ttaaacttct cccttatttt ccattacata
      721 ttgtatgtga ggaaaaacga cttccccatt ataaaggcaa ctattatagc ctgcgttatc
      781 aatatattac tcaatttcat cctgataccg agtatgagca ttatgggagc agcgttagcc
      841 acacttacca gcttcctgat tgtcatgctg atgaaaatgt actatacccg aaaccttccg
      901 gaagccaaac agattattta ccttcgtttt ttaagacaaa gacggtccaa aaaagtaaac
      961 aacgataatt aaacaaccaa aggatgaaga cctacttcga aaatattgaa tggtacgatt
     1021 acctcatttt tttagtttac aatggcgtgt tttcgtattt gatatatcgg ctgggtgatg
     1081 ttttttttaa tatcagcaaa aaagcaacac tagtgcttgt gctcattttt cagttttatt
     1141 tcctattggt tgttggagat agctttttta attatttgcc ctatttaccc gattcggagt
     1201 tatactcctt tatgataagc agcggacaat atccggaaac ttcttctgaa aatatattag
     1261 tgctttatta tgcgacttta ttcataggta ctatttgtct gaacagtccg gtcatttttg
     1321 ttttcttctg tattttttta tatctgatag cactcatgat atttatcagg gcatggaaag
     1381 tggcattgcc aacttttaat gctgcaggag aatttacagc tactttattg atgttggttt
     1441 ggccggcaag tatggtgtat atgacagctc cgttaagaga agcatttatc ctgtttggat
     1501 ttgcattatt ttttaacgga tttattaatt tcttatacaa acaggaatgg aaatcacttt
     1561 taattggcag ccttattatt tgcgccattc gattacaatt aattgtaatg gtatttccgg
     1621 ttttaggtgc tttggcggtt tataaactca aaatcaaccg attggcaaaa gtgagtataa
     1681 tggtgtcaac gctggtaatt gcattattag ctgttcggta tattttagta gaagaaccac
     1741 tttcgcctga aaccatggcc gatttgagaa acagaggaat tctgctggtt ggagaactgg
     1801 gatacggaaa tgtatattgg gattcttatc tggatatggc acgcgattat ccatttatca
     1861 ttttgcagtt tttgttcagc ccgctgccaa tttttgtgca acataatcct attgatacac
     1921 tcattccatt tttggattgt attttcctta tatttttatt tatcgtaata tttcccaaat
     1981 tgcgtttgca tattaaaaac catttgcctc tattattatt cctgctgttt tttattatcc
     2041 tttttggcgg ctacgaatat caccttggtg gtgctgtgcg acatcgaatg cctttggtaa
     2101 taatattgat tttactttcc tcacaaacaa taagcaattt cctttttaag cgtaaacctg
     2161 atgaagaaac tgagcatcct gctgtcaacc attaacgaca gaatttatgc tgcagaacct
     2221 ttactgcaaa atcggtttga ggataccgaa tttgtagtgg tacatcaaat cacgaattta
     2281 agtaaaacag atacttatag taatttttac aataagttcg attcagccta tgttcgcttc
     2341 gttcccatgt ttgataaagg aaccggaaaa agccgcaatt tggccatgca aaaagctgaa
     2401 ggcgaattaa tgtatctctg cgatgatgat ttagtttttc aaccggattt ttatacgaat
     2461 attatgtctg cggcagcggc atttccggat attgatattt ttacttttca ggttctgact
     2521 actgatggcc ttccctttaa acaataccct gaacaagcat atcagcacac ggtaagaact
     2581 actgcaaagg tgagtatagt ggagatggtc attcgcaagc aggcttatgc aacagggaaa
     2641 attaaattcg atgaacgatt tggtctggca acgatttata acaccggtga ggaatttgtg
     2701 atgttaagcg acgcactaaa aaagggcatc actgccaaat ttattcctca atatctggtg
     2761 agccatccgc caatcagctc gggtaaaatt tttaatgagg aagtagcctt tgataaaggt
     2821 gcaatgactg caagggtata cggctggaaa tttatttttg ccaatgccat tttcgctcta
     2881 aaaaaatata ccacatacag aaagtcaatt ggctttttta gctttactta tcatatgtat
     2941 aaaggcagtc tgcatttttt aaaaaatgac taatccgctt gtttccataa ttatgccggt
     3001 ttataactcg gaaaaggtgc tcaaagcggc catcgattct gttttgcaac aaacctatgc
     3061 taactgggaa ttaattattg tagatgataa aagttccgat cagtccgtaa caattatcca
     3121 aacctatgca gctcaaaata ccaacataat tttgatcgaa caagctgtaa atagtggtgc
     3181 tgcgattaca cgtaacaaag gaatctccat cgctaaagga aaatacattg cctttttaga
     3241 tgccgatgat atttggctgc cggaaaaact tgccctgcaa attgcgttta tggaaaaaaa
     3301 tcagtctgct ttaacttgca cttattattc tactatgcag caagatagca caccggtaaa
     3361 gtcaataatt aaagctccgg atcgtatcac ttaccatcat ttacttaaaa ataataccat
     3421 tggttgttta actgccgttt ataatgtgga aatttgtggt aaacaatata tgcccgaaat
     3481 aagaaaacgc caggattacg gtttatggct gaatatatta cgcgcagggt ttgttgctga
     3541 aaccattccg ctggtgcttg ctcaatatcg gattggagcc gattcgcttt cacaaaacaa
     3601 atggaaggtg cttggttata actgggaatt gttgcgaaaa catcagcaat tatcagtggt
     3661 taaatcactc tattatttta cctgtttcct ttggaacaaa acatttaaat atttgaaatg
     3721 aataatcgag caataatttt aggagcatct ggtttaaccg gttcaacatt gttatcactg
     3781 ttatgtgaac aggaatatta tgctgaggta atttgttttg tcagaaaaca aattcacaat
     3841 attcatgata aacaacaaaa tattttgacc gattttaatg accttgaatc gcatgccgca
     3901 ttatttcaac atgcggacgt atattgttgt ctcggcacga ccaataaaaa tgtaaataac
     3961 gataaagaag cttatcgcga agccgattta tacaggccaa tgcgtattgc aaaattactt
     4021 aaggccaata atggcaagca attattaatt atttctgcaa tgggtgctga tgaaagttca
     4081 cgcatttttt ataacaaatt aaaaggtgag ttgcagcgta aattaataga aatgcaatta
     4141 ccttgtttac atatttttca gccatcgtta ttgttagggg acagacgtga acatcgttcc
     4201 ggagaaaaat ttgctacagc actgatggca tttattcaac cgatgttaat aggagggtgg
     4261 agaaaatatc gggctattca tgttaaggct gttgcaaagg caatgtttat taaagcgaaa
     4321 caatcgggct caggcaacca tatttatact tcagatatta ttcagcaaat agcagatgca
     4381 ggataatgta aaatcgcata ttacccaatt tattaaaaca gcagctgcca acgcgggatt
     4441 ttctttttgt ggtattgcga aagccgaaca attgcccgat gaagcgcgta aactggaacg
     4501 ttggctcaat cagggcaaac acggtaaaat gggttatatg gaaaattatt tcgataaacg
     4561 tatcgacccg acacaattaa tgcccggtgc gaaatcggta attagtttgt tatataatta
     4621 ttttcctgag caaacattat cgtcttcttt gaaaattgca aagtatgctt acggtgaaga
     4681 ttatcatgat gtaataaaag aaaagttacg gttattgata gcggaattgc agcacgaaat
     4741 tggagatttt gaagcaagaa tatttgtcga ttcagcacct gttttagaaa aagcctgggc
     4801 gcaaaaaagt ggattgggat ggcaaggaaa aaacacaaat ctcatcaacc ctaaattggg
     4861 ttcattttat tttctggcag aaattatttg tgatttacct ttacattatg accatcctat
     4921 aaaagattat tgcggcactt gtacagcatg tgtagatgcc tgcccaacag atgccctggt
     4981 ggaaccttat cagattgatg catcaaaatg tatttcttat ctcacgatag aattaaaaga
     5041 tgccttgata ccggaacaat ttcatggcaa aatggaaaat tggatttttg gttgtgatat
     5101 ttgtcaagat gtgtgcccat ggaaccgatt tgcgaaacaa catcaggaac caaaattttt
     5161 tccaaccgac gatttaaaaa atgttgccgc aaatgactgg aaagaagtta ctgctgaaat
     5221 atttaatcag gtgtttaaac attccgcaat caaacgcaca aaatggagcg gatgggaaag
     5281 aaatattatt acagcaaaaa aataattagt gaatacgctc tccactgagt tgcatatttt
     5341 tcatgactga agcttcaaat tcgagccatt cctgccatct tttttttact ttatcggggt
     5401 tgatatagtt attggctaat gaaataaaca tcttgtaatg tccggcctca cttaccataa
     5461 attcatgata aaaattacgc atttcctcat ctgctaatcc ctcacttaat aaacggaacc
     5521 gctcacaact tctcgcttct ataagtgcag ccatcagcag tttttccaat aattggtctt
     5581 cacggctgcc cccttttttc tgaaatttga ttaattcttt cacatattca tcgctgcgct
     5641 gtttgcccta tttcaaatcc cgctttttca gctcttttaa taccattcta aaatgtcccc
     5701 attcttcggt aaccaccggc gacacttcgt ccaccaggcg ctccctgtcc ggaaataatt
     5761 ggataagcga tatacaggta gatgctgcct tttgctcaca ataggcatgg tcggtgagta
     5821 tttcctcaaa acttttttcc gcaagattca cccaacgcgg gtcagtgggt aatttta
//
LOCUS       DAORKW010000193         5873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_524_length_5873_cov_4.440873, whole genome shotgun sequence.
ACCESSION   DAORKW010000193 DAORKW010000000
VERSION     DAORKW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_524_length_5873_cov_4.440873"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..3590)
                     /gene="nifJ"
                     /locus_tag="PLB46_08785"
     CDS             complement(9..3590)
                     /gene="nifJ"
                     /locus_tag="PLB46_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRF76652.1"
                     /translation="MSDILETVKPIETAQNEIESPVRDIMDGNEAAATIAYKTNEICA
                     IYPITPSSAMGEFADEWSSKELTNIYGQVPKVIEMQSEAGAAGAIHGALTGGALSTTF
                     TCSQGLLLMIPNMYKIAGELTPTVFHIAARALATHALSIFGDHSDVMACRSTGFSMLF
                     GNNAQEAMDMALIAQSATLKSRIPFLNIFDGFRTSHELTEVEIIPDSVIRKMINLDDV
                     MAHRRRSLNPANPFIKGTAQNPDVFFQSREAANKYYLNVPFVVEQTMQQFAQLTGRKY
                     KLYEYYGAEDAEKIIIIMGSGSGAVHEVVDYLNANGQKTGVLTIRLFRPFYIASFIQA
                     VPKSVKKIAVLDRCKEPGAIGEPLYLDVINAFNEGWDAATPKIIGGRYGLASKEFNAA
                     MVKAVFDELDAPKPKNHFTIGINDDVTNTSLAVDQSFSLEAQHYFRGLFFGLGADGTV
                     SANKNSIKIIGDVTGDYVQGYFVYDSKKSGSLTVSHLRAGKEPIRSTYLINSANFIAC
                     HHFNYMKKYDILKDAEQGATFLLNAPYPKDEVWSMLPEKIQEEIIHKQLKLYVINATA
                     VAKETGMGSRINTILQTCFFAISNVMPKEVAIGKIKAAIYESYYKKGEKVVQKNYEAV
                     DKTLENLFELHYAQYAIGKIQIDAPVPDNSPDFVKSVLAKIIAGEGDGLPVSAFPVDG
                     LYPSGTTKFEKRNIAENVPVWDADLCSQCGKCFLICPHAAIRCKVYEKEELQHAPTTF
                     KYVDPIGKEFFKDHEAYTLQVSVEDCTGCNLCVEYCPIESKTQPGHKAINMVDKMELV
                     EPERENWDFFLSLPDIDRTRVNKNTVKGTQFLQPLFEFSGACAGCGETPYLKLLTQLF
                     GERMIVANATGCSSIFGGNLPTTPWSQNNEGEGPAWANSLFEDNAEFGLGIKLATDKK
                     REIARQLLSEMREEVGEGLADNILAVHETDESAIKNQKKDIIELKKKLRDINHINAKI
                     LLQLADFLAEKSIWIIGGDGWAYDIGFSGLDHVLSTGENVNIFVLDTEVYSNTGGQKS
                     KASPLGASAKFSINGKRTGKKDLALQAIAHGKAYVAQIAMGANDMHTVKTIQEAEAYP
                     GPSIIIAYSHCIAHGYDMCHGNDQQKNAVNSGYWPLFRYNPLKAKGERFVLDSKAPTL
                     PLGEFMYNENRFNLIKAKDAELADTFLHEAEHDIHDRYERLNLLKGL"
     gene            complement(3966..4478)
                     /locus_tag="PLB46_08790"
     CDS             complement(3966..4478)
                     /locus_tag="PLB46_08790"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRF76653.1"
                     /translation="MSSKKVADSFTTFTEIVMPNDTNPMHNLMGGNLLKWMDIACGIC
                     GGKHANKIVVTAAVDNVSFSRPIKIGDIVTITAQVTRAFNTSMEIFVEVFKENFRSSE
                     KVKCNEAFLTFVALDDDGRPVTIPELIPETEDENRRYISALRRRELRLVLAGRMKPEE
                     AKELKEIFLQ"
     gene            4697..5572
                     /gene="ygiD"
                     /locus_tag="PLB46_08795"
     CDS             4697..5572
                     /gene="ygiD"
                     /locus_tag="PLB46_08795"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HRF76654.1"
                     /translation="MNRRKFIQAGALGFGGLGLLSSMQLHQFKDQFSEETDVTMPVLF
                     VGHGNPMNAIEDNEFSQGWAATGKLLTVPKAILCVSAHWLTRGTRVCAVANPETIHDF
                     GGFPQALFDVQYNAPGSPEFAATTQQLITKTKVELDNEWGLDHGCWSVLNKMFPAANI
                     PVFQLSIDYYQQPQYHYELAQELKALRKKGVLIIGSGNIVHNLRLFTMQDIAYDWAIE
                     FDTKIKELLEKGDHTPIINYKTLGKAAELSVPTNDHYLPLLYTIALQEKNESLRFFND
                     KTTAGSISMRSLIIG"
BASE COUNT         1803 a         1174 c         1004 g         1892 t
ORIGIN      
        1 cccggcaatc acagcccttt taataaattc aatcgctcat acctatcatg aatatcatgc
       61 tccgcttcat gtaaaaatgt atcagccagt tccgcatctt ttgctttaat taaattaaac
      121 cggttttcat tatacataaa ttcacctaaa ggcaatgttg gcgcttttga atctaacaca
      181 aaacgttccc cttttgcttt caacggatta tatctaaaca aaggccaata acctgaattc
      241 accgcatttt tttgctggtc gttaccatga cacatatcat aaccatgagc aatacaatga
      301 ctgtatgcaa ttattatgga tggtcccgga tacgcttcag cttcctgaat tgtttttacg
      361 gtatgcatat cgttagcacc catggcaatt tgtgctacat aagcttttcc atgtgcaata
      421 gcctgcaatg ctaaatcttt tttaccggta cgttttccat ttatcgaaaa tttcgcactt
      481 gcccctaatg gcgaggcttt ggatttttgt ccaccggtgt tagaatacac ttctgtatcc
      541 aacacaaaaa tattgacatt ttcgccggtt gataacacat ggtctaaacc gctaaatccg
      601 atatcgtaag cccaaccatc accaccaata atccagatag atttttcagc cagaaaatca
      661 gcgagttgta ataatatttt tgcattgata tgattaatat cgcgcaattt ctttttcaat
      721 tctataatat cttttttctg atttttaatt gcactttcat cagtttcatg taccgctaaa
      781 atattatctg ccaacccctc acctacttct tcacgcattt ccgataataa ttgtctggca
      841 atttcacgtt ttttatctgt tgccagttta atgcccaatc caaattcagc attatcttca
      901 aacaacgaat ttgcccaggc aggcccttcc ccttcattat tttgcgacca aggtgtggtt
      961 ggtaaattac cgccaaaaat agatgaacaa ccagttgcat tggcaacaat catcctttca
     1021 ccaaataatt gagtgagtaa ttttaaataa ggtgtttcac cacatccggc acaggcacct
     1081 gagaattcaa ataatggttg taaaaactgt gtacctttta cggtattttt atttactctt
     1141 gttctgtcga tatccggtaa tgataaaaag aaatcccaat tctcgcgttc aggttcaacc
     1201 aattccattt tatcaaccat attaattgcc ttatgacccg gctgagtttt actttctata
     1261 ggacaatatt ccacacataa attacatcct gtgcaatctt ctacactcac ttgtaatgta
     1321 taagcttcat ggtctttaaa aaattctttt cctattgggt caacatattt aaatgttgta
     1381 ggagcatgtt gtaattcttc tttttcatat actttacaac gaatggcagc gtgcggacaa
     1441 atgaggaaac atttaccaca ttgtgaacat aaatctgcat cccaaaccgg aacattttct
     1501 gcaatatttc gtttttcgaa tttggttgtt cctgaaggat ataatccatc gacaggaaaa
     1561 gcgctaaccg gtaaaccatc gccttctccg gcaataattt tcgccaatac cgatttaaca
     1621 aaatcaggtg aattatccgg aacaggagcg tctatttgaa ttttcccaat tgcatattgt
     1681 gcataatgta attcaaataa attttccagt gttttatcta ccgcttcata atttttttga
     1741 actaccttct cccctttttt ataataagat tcataaatag ctgcttttat tttaccaata
     1801 gccacctctt ttggcattac attactaatg gcaaaaaaac atgtttgtaa aatagtatta
     1861 atacgcgaac ccatgccggt ttcttttgct acagcggttg cattaattac gtataatttt
     1921 aattgtttat gaataatttc ctcttgaatt ttttcaggca acatcgacca tacttcatct
     1981 ttcggataag gtgcatttaa caaaaatgtt gcaccctgtt ctgcatcctt caagatgtcg
     2041 tattttttca tgtaattaaa atgatggcat gcaatgaaat tggcagaatt aatcaaataa
     2101 gtagaacgaa ttggttcttt gcctgcacgc agatgagaaa cagttaaact gccggatttt
     2161 ttcgaatcat atacaaaata cccctgcaca taatcaccgg ttacgtctcc aataatttta
     2221 atggaatttt tatttgcact tacagttcca tcagcaccta atccgaaaaa taatccgcgg
     2281 aaataatgtt gagcttctaa tgaaaaactt tgatcaactg ctaaactggt attggtaaca
     2341 tcatcgttaa ttccaatagt gaaatgattt ttaggttttg gcgcatccag ctcatcaaat
     2401 actgctttta ccatagctgc attaaattct tttgaggcta atccataacg tccgccaata
     2461 atttttggag ttgcagcatc ccatccttca ttaaaggcgt taattacatc taaataaagt
     2521 ggttcgccta ttgcacccgg ttctttacaa cgatctaaaa cagctatttt tttaactgat
     2581 ttaggaactg cctgaatgaa tgaagcgata taaaatggac ggaataaacg aatggtgagc
     2641 acaccggttt tttggccgtt tgcatttaaa taatcgacca cttcatgaac agcaccggaa
     2701 ccggagccca tgatgatgat tattttttct gcatcttcag caccataata ttcatataat
     2761 ttgtatttgc gtccggtgag ttgtgcaaat tgttgcatag tttgttccac cacaaatggc
     2821 acatttaaat aatatttatt ggcagcttcg cgactttgga aaaatacatc cggattttgt
     2881 gcagttcctt taataaaagg attagcagga tttaatgagc ggcggcgatg tgccatcaca
     2941 tcatccaggt ttatcatttt gcgaataaca ctatccggaa ttatttccac ttcagttaat
     3001 tcatgagaag tgcggaatcc atcaaaaata tttaaaaaag gaatgcgtga ttttaatgta
     3061 gcggactgtg caattaatgc catatccatt gcctcttggg cattgttgcc aaacagcatg
     3121 gagaaaccgg ttgaacggca tgccataaca tcactatggt caccaaaaat ggataaagca
     3181 tgcgtagcta atgctcttgc tgcaatatga aatacagtag gtgttaactc acctgcaatt
     3241 ttatacatat tgggaatcat cagtaataaa ccctgtgaac aggtaaaagt agtagacaaa
     3301 gccccacctg ttaatgcacc gtgaatggca cctgcagcac cggcttcact ttgcatttcg
     3361 ataactttag gtacttgccc ataaatattg gtgagctctt ttgacgacca ttcatctgca
     3421 aattcaccca ttgccgatga aggtgtaatg ggataaattg cacaaatttc attggtttta
     3481 taagcaatgg tagcagctgc ttcattgcca tccataatat cgcgaaccgg tgattctatc
     3541 tcattttgag cagtttcaat tggttttact gtttcaagta tgtctgacat aatttacgat
     3601 tttggtggtt aaattacggg ctgacccgaa attgaagtaa acctctcaag aaatcagttt
     3661 acaccacatg acagacgtca tttacggagg tgataaaagt caatccggag acaaatggta
     3721 tatgaaaatg gaataaaacc tatggctttt ggaggattgc gaagtaggcc aaattcagct
     3781 tccaatttta tgtcaaaaca acataagttg cactactaaa tcaggtgtta tagatttaac
     3841 tttaggaata gttcagaaat taaattacaa ataccttgac acccttatga gcatttacta
     3901 cttattattt tttcaaccat aaagtctcac aaaagcaaca aattaaatga taaaacaaaa
     3961 ataaattact gcaaaaaaat ctcttttaat tcttttgctt cttcaggttt cattctgcct
     4021 gctaacacta aacgcaattc acgacgcctt aatgcagaaa tataacgacg gttttcgtct
     4081 tcggtttcag gtattaattc aggaatagtc acagggcgac catcatcatc taaagcaaca
     4141 aaagtgagaa atgcttcatt acattttact ttttctgatg agcggaaatt ttctttaaaa
     4201 acttccacaa aaatttccat actggtatta aaagctctgg ttacctgagc tgtaattgtc
     4261 acgatatctc ctattttaat agggcgactg aatgaaacgt tatctacggc ggcagtaacc
     4321 acaattttat tagcgtgttt accaccgcaa ataccacagg caatatccat ccatttcagc
     4381 aaattaccac ccatcaggtt atgcatcggg ttggtgtcgt tgggcataac tatttcggtg
     4441 aatgtggtaa acgaatcggc aactttttta ctgctcataa ctttacaaat tgagacacaa
     4501 aggtaataac ttatcggtgg tgactgcaat tgcctgagtt ttttaaccag agtaaagcat
     4561 tttattgcaa ttgtatggtt gaaagggcat tttccatccc caaatagcag cttttgaact
     4621 tttttttgct gcccggcgtt caaaatgtat gtatatacat atatatgtgg aaatatttat
     4681 ttaattttac ctactaatga accgccggaa atttatacaa gcaggcgccc tcggttttgg
     4741 gggattagga ttattgagct ctatgcaact acatcaattt aaagatcagt tttcggaaga
     4801 aacagacgtg accatgccgg tattatttgt cggccatggc aaccctatga atgccattga
     4861 agacaatgag tttagtcaag gttgggcagc gacaggaaaa ttacttacgg ttcctaaagc
     4921 cattttgtgt gtatcggcac attggttaac cagaggtaca agggtatgtg cagtcgcaaa
     4981 tcctgaaaca attcatgatt tcggtgggtt tccgcaagca ttatttgatg tgcagtataa
     5041 cgcaccgggc tcacctgagt ttgccgcaac tacgcaacaa ttaatcacca aaacaaaagt
     5101 ggaactcgac aatgaatggg gtttggatca tggctgttgg agtgtattaa ataaaatgtt
     5161 tccggctgcc aatattccgg tatttcaatt gagtatagat tattaccaac aaccacaata
     5221 tcactacgaa ttagcgcagg aattaaaggc attgagaaaa aaaggtgttt taattattgg
     5281 cagtggaaat attgtacaca atttacgttt atttaccatg caggatatcg cctacgattg
     5341 ggcgatagaa tttgatacca aaataaaaga gcttttagaa aagggcgatc atacaccaat
     5401 tattaattat aaaacgcttg gtaaagcagc agaattatct gttcctacca acgaccatta
     5461 tttaccattg ttgtatacca ttgcactaca ggaaaaaaat gaatcgcttc ggttttttaa
     5521 tgataaaact acagcaggtt caatttcaat gcgctcatta ataattggat aattatttga
     5581 ggtaaaaagc tgtattgatt aattcaggtt ttgtatttgc tgttttgcac tatcatttaa
     5641 ttacaatttc ataacagcat ccaaaccccg atacagacct tttaatccaa ttaccttacg
     5701 cagcgccagc aaagccccat ctatataggg ttttgtcggg tcaatggcat catgtcgtat
     5761 ttttaatctt tcggctccgg ttccaaatat aatttcacac gagttgttga aacccggtca
     5821 gcgtacagca tgaatttgtg tgccttcaac attcgctcca cggctttcag ttt
//
LOCUS       DAORKW010000194         5872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_525_length_5872_cov_5.762592, whole genome shotgun sequence.
ACCESSION   DAORKW010000194 DAORKW010000000
VERSION     DAORKW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_525_length_5872_cov_5.762592"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLB46_08800"
     CDS             complement(<1..382)
                     /locus_tag="PLB46_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF76655.1"
                     /translation="MAEITEKTITLKGGEFLIKDATPQDIFTPEELNDEQLMIKQMTK
                     DFVDTEVIPNLDRIDNHEPGLMASLLDKAGDIGILGLPIPEAYGGFNKDFNTISVVTE
                     MTGTAHAFSVAYGAQSGIGTLPILS"
     gene            complement(420..1598)
                     /locus_tag="PLB46_08805"
     CDS             complement(420..1598)
                     /locus_tag="PLB46_08805"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRF76656.1"
                     /translation="MQEAYIVAGLRTAVGKAPRGLFRFTRPDDLAVEVIRQLLLKVPQ
                     LDPARVEDLIVGNAVPEAEQGLQIGRMIAVRALPLTTGGMTVNRYCASGVETIAIATA
                     KIKSGMAVCIIAGGVESMSLVPTAGWKVALNYETAKEHPDYYVGMGLTAEAVAQQFNI
                     NRTDADQFAYESHQKAIHAIEQGYFKDDIAPIEVTEVYVNEKNKRAEKKYTVTTDEGP
                     RKDTSLEALAKLKPVFANGGIVTAGNSSQTSDGAAFVIVMSERMVKELNLQPIARMVS
                     CATVGVEPRIMGIGPVAAIPKALKLAGMQLSDIDLIELNEAFACQALAVIREAGLDPA
                     KVNINGGAVALGHPLACTGTKLTVQIAADLKRLNKKYGMVTACVGGGQGIAGIYERIN
                     "
     gene            complement(1623..4010)
                     /locus_tag="PLB46_08810"
     CDS             complement(1623..4010)
                     /locus_tag="PLB46_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRF76657.1"
                     /translation="MNRIIKKVAVLGSGVMGSRIACHFANIGVQVLLLDIVPLQLTDE
                     EKKNPTLRNKLVNDSLQAAIKQNPSPVYAQKVIKQITTGNFDDDLQKIKDVDWIVEVV
                     IENLEIKQQLFEKVDAIRKPGTLITSNTSGIPIHQMIIGRSDDFKQHFCGTHFFNPPR
                     YLKLLEIIPSPNTDKHVVDFLMDYGDRFLGKTTVLCKDTPAFIANRIGVFSIMAVFNV
                     MEKLGLNIDEVDALTGPISGRPKSATFRTADVVGIDTLVKVAKNTYTDCPNDESRDMF
                     IVPAYVEKLIENKWLGDKTGSGFFKKVKNPEGKSEIHVLNLKTFEYTPSVKAKFATLD
                     AAKQIDDVKSRIKALHHGTDKAGDFYRNVTALVNKYVSFRIPEIADELYKIDDAVKAG
                     FGWELGPFETWDVLGVEKTAQMMEASGFAPAAWVKEMTAAGNKSFYITENGKRKFYDV
                     ATKSYKDIPGADAFILLDNYRTQKPVFQNAGATLFDIGDGILNLEFHTKMNVIGSEIL
                     EAMHKSIDIAEKNYRGLVIGNDAANFSAGANIAMMLMLAIEQEYDELDMAIRYFQNTI
                     MRVRYSGIPVAVAPHGLTLGGGCEMTMHSDVAVAAAETYIGLVEVGAGLIPAGGGTKE
                     LALRASDKYFKGDIEIPTLQEMSLNIAMAKVATSAREAFDLGILRKGIDIEVMNSNRV
                     IADAKNAVLELANNGYTPPQQRSDIKVLGRSALGSFYTGIASMRMGNYISAHDEMIAK
                     KVCYVICGGDLSAPTEVNEQYLLDLEREAFLSLLGEKKTLERMQFILKTGKPLRN"
     gene            complement(4074..4664)
                     /locus_tag="PLB46_08815"
     CDS             complement(4074..4664)
                     /locus_tag="PLB46_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF76658.1"
                     /translation="MPVKKVEKFDIIKQALNLFRTQGYHKTTMANIGDACGLLKGSVY
                     HYFSSKEDLMREVLEVLRDHYRDKVFIYAYDETKSAFERLRLLGEKSIEVFVNGEGAC
                     LMANIAMETNDVVPEFRKPIRDFFDEWVNALAFIYSDRYSQEAARIFAQESVCAIEGA
                     AMMMRIYNDKNYLLTAHAMIMGKFEKADKEALISSK"
     gene            complement(4771..5187)
                     /locus_tag="PLB46_08820"
     CDS             complement(4771..5187)
                     /locus_tag="PLB46_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76659.1"
                     /translation="MRNLRILPVAVIAMVLITSCTKTDMNTISIAEAESIIPGSYKVT
                     DFAGVGDLSQYQDYTFEFLSNGDIRATKGAESYTGSWDITNVADATYDKEVSITIAGN
                     EQMDMLNHSWFVKDVTDVTLNLVDDTATEEFLFIKI"
     gene            complement(5410..5835)
                     /locus_tag="PLB46_08825"
     CDS             complement(5410..5835)
                     /locus_tag="PLB46_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76660.1"
                     /translation="MKKIILMSGIVLSVFMMPSCSDDSLDDVTVTEAEALAGGSWKVV
                     YYFDNGDGVSNDFDGYTFEINDDGTLSASKSGTTYTGTWMVKSSDDDPDYDKEIEFTI
                     TGNKQMDDLDGSWLIAELTDSSMKLKDDTPSEEIHFEKI"
BASE COUNT         1695 a         1237 c          971 g         1969 t
ORIGIN      
        1 aagataaaat tggcaatgta ccaattccac tttgagcgcc ataagcaaca gaaaacgcat
       61 gtgcagttcc tgtcatttca gttactacag aaatggtatt aaaatcttta ttaaatccac
      121 cataggcttc cggaattggc aatcctaaaa ttccgatatc accggcttta tctaaaagcg
      181 atgccattaa tcccggttca tgattatcga tacgatcgag gttgggaata acttcagtat
      241 ccacaaaatc ttttgtcatt tgtttaatca tcaattgttc atcattcaat tcttcgggag
      301 tgaagatatc ctgaggtgtt gcatctttaa taaggaattc accgccttta agggtgattg
      361 ttttttctgt aatttcagcc atgacagtta atatttataa gtttataaaa ttcaattaat
      421 taattaattc gttcataaat tccggcaatt ccctggccac cgcctacgca agcagttacc
      481 attccatatt ttttatttaa acgttttaaa tcggcagcaa tttgtactgt taattttgtt
      541 cctgtgcatg caagcggatg acctaaagcc actgcgccgc cattaatatt aacttttgcc
      601 gggtcgagac cagcttcgcg aattactgct aaagcctgac aagcaaatgc ttcgtttaat
      661 tcgattaaat caatatcact taattgcata ccagccaatt ttaacgcttt aggaattgca
      721 gcaacaggac ctattcccat aatacgaggt tcaacgccaa ctgtagcgca acttaccatt
      781 cgtgcaattg gttgcagatt taattctttt accattcgtt cgctcataac aattacaaac
      841 gcggcaccat cggaagtttg tgatgagttt ccggcagtaa caattccacc attagcaaaa
      901 acaggtttta attttgcgag cgcttcaagt gaagtatctt ttcgcggacc ttcgtcggta
      961 gtaactgtat attttttttc tgcgcgttta tttttttcgt tgacatatac ttctgttact
     1021 tcaattggtg caatatcatc tttaaaatat ccctgttcga tggcatgaat tgctttttga
     1081 tgtgattcat aagcaaattg gtcggcatcg gtgcgattaa tattaaattg ttgtgcaact
     1141 gcttcggcag ttaatcccat acccacataa taatcaggat gttcttttgc agtttcataa
     1201 tttaatgcca ctttccaacc tgcagttggc accaggctca tactttctac accacctgca
     1261 ataatacaaa cggccatacc tgatttaatt tttgctgttg caattgcaat ggtttcaaca
     1321 ccacttgcgc aatatctgtt aaccgtcatc ccaccggtag ttaatggcaa tgcacgaacg
     1381 gcaatcatac gaccaatttg taatccctgt tctgcttcag gtaccgcatt accaacaatt
     1441 aaatcttcca cacgggcagg atctagttga ggaactttta ataataattg tcgtatgact
     1501 tcaacagcca aatcatccgg tctggtaaaa cgaaataatc ctcttggtgc tttgccaact
     1561 gctgttctta atcctgcaac gatatacgct tcttgcataa taaatggtat taattgttat
     1621 aattaatttc ttaaaggttt acctgttttt aaaataaact gcatacgctc caatgttttt
     1681 ttctcaccca ggagggataa aaatgcttcg cgttctaaat ccagtaaata ttgttcattc
     1741 acttctgtag gtgcacttaa atcgccacca caaattacat aacacacttt tttcgcaatc
     1801 atttcatcgt gggcagaaat ataattaccc atgcgcattg acgcgatgcc ggtgtaaaaa
     1861 cttccgagtg cacttcgtcc taaaacttta atatcgctgc gttgttgcgg aggagtatat
     1921 ccattattcg ctaattccaa caccgcattt tttgcatcgg caattacacg attgctgttc
     1981 attacttcta tatcaatccc ttttcttaaa atgcctaaat caaaagcttc acgtgcactg
     2041 gtggccactt tagccatggc aatatttaaa ctcatttcct gtaatgttgg aatttcgata
     2101 tcgcctttaa aatatttatc acttgcacga agagctaatt ctttagttcc tccaccggca
     2161 ggaattaatc cggcccctac ttctactaat ccgatatatg tttctgctgc ggcaacggca
     2221 acatcgctat gcattgtcat ttcgcatcca ccaccgagag ttaaaccatg tggtgcaacg
     2281 gcaaccggaa tgccggaata acgcactcgc ataattgtat tttggaagta acgaatagcc
     2341 atatccagtt cgtcatattc ctgttcaatt gcgagcatca gcatcatcgc gatatttgcg
     2401 ccggctgaaa aattggctgc atcgttacct attactaatc cgcgataatt tttttccgct
     2461 atgtcgattg atttatgcat agcttccaaa atttcgctac caataacatt cattttggta
     2521 tgaaattcca gatttaaaat accatcacca atatcaaaca aagtagcgcc tgcattttga
     2581 aataccggtt tttgtgtgcg gtaattatct aacaaaataa atgcatcagc acccggaata
     2641 tctttgtatg attttgtagc tacatcataa aactttcgtt ttccattttc cgtaatataa
     2701 aatgatttat tacctgcagc ggtcatttct ttcacccatg ctgccggagc aaaaccactg
     2761 gcttccatca tttgagcagt tttttctacg ccgagtacat cccatgtttc aaatggccct
     2821 aattcccatc caaaaccggc tttaactgca tcgtcaattt tatataattc atcagcaatt
     2881 tccggaatac ggaatgatac atatttatta accagtgctg ttacattgcg ataaaaatca
     2941 ccggctttat cggtgccatg atgcaaggct ttgattcttg attttacatc atcaatttgt
     3001 ttagcagcat caagtgtggc aaattttgct ttaacagaag gcgtgtattc aaacgttttt
     3061 aagttgagta catgtatttc actctttcct tccggatttt ttactttttt gaaaaagccg
     3121 cttcctgttt tatcgcccag ccatttattt tctattaatt tttctacgta agcaggcaca
     3181 ataaacatat ctctgctttc atcatttgga caatcggtat aagtattttt cgcaacttta
     3241 actagcgtgt cgataccaac aacatcggca gttctaaaag ttgccgattt tggtcttccg
     3301 ctaattgggc ctgttagtgc atcaacttca tcaatattca accccaattt ttccatcaca
     3361 ttaaataccg ccataatact aaacacacca atacgattgg caataaaagc aggcgtgtct
     3421 ttacataata ccgtagtttt tcctaaaaag cgatcgccgt aatccattaa aaaatctact
     3481 acatgtttat cggtattcgg agaaggaatt atttccaata atttcaaata acgaggagga
     3541 ttaaaaaagt gagttccaca aaaatgttgt ttaaaatcat cacttctacc aattatcatt
     3601 tggtgaattg gaatgcctga tgtgttggat gtaattaatg ttcccggttt acggatagca
     3661 tcaacttttt cgaacagttg ttgttttatt tcaagatttt cgataacaac ttcaacaatc
     3721 caatccacat cttttatttt ttgcaggtcg tcgtcgaagt taccggttgt aatttgttta
     3781 attacttttt gagcatatac cggagaagga ttttgtttaa tagctgcttg caaactatcg
     3841 tttaccaatt tattgcgcag cgttggattt tttttctcct catcggtaag ttgcaaaggc
     3901 acaatgtcaa gcagtaacac ttgaacgccg atattggcaa aatggcatgc gatgcggctt
     3961 cccataacac ccgaacctaa aactgcaacc tttttaatta ttctgttcat tgattcaata
     4021 tttaaaacac aacattaatt attacctatt attttaattt aaccccagta ttattattta
     4081 ctgctgatta aagcttcttt atctgctttt tcgaatttgc ccataatcat agcatgtgca
     4141 gtgagcaaat aatttttatc gttataaatg cgcatcatca tggcagcgcc ttcaattgca
     4201 caaactgatt cttgcgcaaa aatcctggcg gcttcctgac tatatctatc gctataaata
     4261 aaagcgagtg cattaaccca ttcgtcgaaa aaatcgcgta taggtttgcg aaattcagga
     4321 accacatcat ttgtttccat tgcgatattt gccatgaggc aggcaccttc tccattcaca
     4381 aatacttcaa tacttttttc accgagtaaa cgaagtcttt caaaagctga ttttgtttcg
     4441 tcgtatgcat aaataaatac cttatcgcga taatggtcac gaagcacttc aagtacttca
     4501 cgcatcaaat cttctttaga tgaaaaatag tggtatacgc ttccttttaa caaaccacaa
     4561 gcatcaccga tgttagccat ggtagtttta tggtaacctt gcgttctaaa caggttgagg
     4621 gcctgtttaa tgatatcgaa tttttctact tttttaaccg gcatagaaca aagatattgg
     4681 cttttttgaa tttaccaaac gtttgtttga taaaaacggt aaaaaaaatg gtccagccct
     4741 ccggctgaac catttcacgt tatcgtcaga ttagatttta ataaacaaaa attcctctgt
     4801 agcagtgtca tctacaaggt tgagcgtaac atctgtcaca tctttcacaa accagctatg
     4861 gtttaacata tccatttgct cgtttccggc aatggtaatt gacacttctt tgtcgtaagt
     4921 agcatctgca acattggtaa tatcccagct acctgtatac gattcagcac ctttagttgc
     4981 acgtatatcg ccattactta aaaattcaaa agtgtagtcc tgatattgtg ataagtcgcc
     5041 aacaccggca aaatcggtca ctttgtacga acccggaata atactttcgg cttcagcaat
     5101 ggaaatggtg ttcatatcgg ttttagtgca agaagtaatt aataccattg caatcacggc
     5161 tactggaagg attcttaagt ttctcataat aagcttgttt tgttgtctgt aaattaaatg
     5221 gattttagcg gtatgtcgtt gcatgggcgc ctaaggcagc atgtcacatt cgcaatcaat
     5281 tcattttcag cacctccttt attttaaagt caaaataact tcctgactgt taaaataact
     5341 caatatgctt gtcacttatt gtatccaggt gaaaataata tgattgacct atgtaaacta
     5401 cagtagggat tatatttttt caaagtgaat ttcttcagat ggtgtatcgt ctttgagttt
     5461 cattgacgaa tcggttaatt ctgcaattaa ccagctgcca tccaggtcgt ccatttgttt
     5521 gttgcctgtt atggtaaatt caatttcttt atcgtaatca ggatcatcat cgctgctttt
     5581 caccatccag gttccagtgt acgttgttcc gcttttgctg gcagatagag ttccgtcatc
     5641 attaatttcg aaagtatagc cgtcaaaatc attactaaca ccgtcaccat tatcgaaata
     5701 ataaaccact ttccacgaac cacctgcaag tgcttcagct tcggttaccg ttacatcatc
     5761 taaggagtcg tcagaacaag agggcatcat aaatactgat aatacaatgc cggacattaa
     5821 gattattttt ttcatacgtt taaatttttg tttgttgaat ttgttgttgt tt
//
LOCUS       DAORKW010000195         5856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_528_length_5856_cov_4.221858, whole genome shotgun sequence.
ACCESSION   DAORKW010000195 DAORKW010000000
VERSION     DAORKW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_528_length_5856_cov_4.221858"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLB46_08830"
     CDS             <1..544
                     /locus_tag="PLB46_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF76661.1"
                     /translation="FASHYCETRPDIMCISKGITGGTMALGATLCATPIFDAFYSDDK
                     MKALYHGHSYTANPLACTAANASLDIIEQAGFIDNVISITSIQSDFAERLKTFVTATT
                     KQVTNIRQHGTILAFDVVTPHQTGYFNELRDEMYRFFIGNGVLLRPLGNTVYIMPPYC
                     INVEQMVKIHAVILAYLAQP"
     gene            complement(545..1327)
                     /locus_tag="PLB46_08835"
     CDS             complement(545..1327)
                     /locus_tag="PLB46_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRF76662.1"
                     /translation="MNWSTITYAVHQKVGSITLNRPDKRNAFNATLVAELKAAFAMAE
                     GDTQVKVIVLKGNGEVFSAGADLGYLQSLQNNSYQENLEDSLSLMELYKQIYTLSKPV
                     IAQIEGHAIAGGCGLATICDFSFAVDEAKFGYTEVGIGFIPAIVMVFLLRKIGERNAK
                     ELLLTGKLISAAEAKQMGLINDVVGVENIEATVEQLCQLLIDKTSRASLQATKQMIAA
                     VQEMGLNEALHYAAEMNAKARATDDCKHGINSFLNKQKPNWD"
     gene            complement(1350..3746)
                     /locus_tag="PLB46_08840"
     CDS             complement(1350..3746)
                     /locus_tag="PLB46_08840"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRF76663.1"
                     /translation="MKISYTLICLLFPLFLFSQNVTQTIRGHVSDKDSKFPVLGATVL
                     VYADSVMVGGGVSDESGDFRVTNVPVGRVNMVFRSVGYTDLPVSNLIITGGKEVILNV
                     ELEEQTILLEGLEIKAYQDKREVNNEMAVVSVRTFSVEETDRYAGSRGDPARMASNFA
                     GVQGADDSRNDIVVRGNSPIGVLWRLEGVDIPNPNHFAIAGTTGGPVSIINNKYLSNS
                     DFFTGAFPAEYGNSIAGVFDLNMRRGNNEQHEFSGQLGFLGTELFAEGPINKSNKSSY
                     LASFRYSTLQLFQFMNLSIGTSAVPNYFDGAVKLSFPKGTSIINVFAIGGKSNIDILV
                     SEYNKAETELYGDSDRDQYFGTSMSVAGVSYGMQINPKAYGKTVVAYSWQENHANHIL
                     VYRDTLFEIDSMVPNMNYSYKDQKITALYHLNYKVNNRHSFKAGVSAEQYMYNHIDSN
                     LNLFTWDFENRYDYTDNALLFQAFFQWKYKAAEKLVVNGGLHGQLFTINSNSKSIEPR
                     LGLSYTLTEKSSLNFGAGLHSQMLPSYQYTYQQIQPDNSFILINEDVGFIRSFHSVLG
                     YDQMLGKNFRLKMETYFQYLYDVPVEVQLSAFSLANEGSGFSRFFPDSLQNTGTGKNY
                     GVELTLEKFFSNHFFFLATGSLYNSLYVGSDGIERKTDFNGSYAANLLGGYELTISDR
                     NVITTGIKMTYAGGKLSTPVDTMATIAAKELIFIDSLTNTLQLKDYFRFDLKLAYRIN
                     SKKNKVTHEIAIDLVNLFDVQNVLGLTYAPNPADITVSPIKEEYQLGFLPLFYYKIDF
                     "
     gene            complement(3892..4449)
                     /locus_tag="PLB46_08845"
     CDS             complement(3892..4449)
                     /locus_tag="PLB46_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76664.1"
                     /translation="MKKGFLAVMAITTALFVTSCGKKGGLSEETKAAMQTFETNWKAT
                     EGNMTAMGTTMTTAFGEMTTMMAANDAMDMSKLKPATKASVDSMLTMCTMIKGQMEKM
                     KGDYSAAMTQWAADATAYAEWKKKAQEEKLADAEFKAELDNNWNAKLATYNDQLVAMN
                     ESLTGIQAACKSSCDAITGMVTPTK"
     gene            complement(4630..5580)
                     /locus_tag="PLB46_08850"
     CDS             complement(4630..5580)
                     /locus_tag="PLB46_08850"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRF76665.1"
                     /translation="MGILLCSPAGNDIIYDDKLIEQGRNFNNKLWNVLRLIKGWEISG
                     ENNTENTAAIEWFESKLNHTIATIDDHFTHFRISDALMTLYNFIWDDFCSWYLEFVKP
                     EFGKPIDKNSYDKTIEFFEQCLVLLHPYMPFITEELYHLLQDRNAGDDIIMARLPKGG
                     STNENLIETGEAVKDIISKVRDIRAKNNMSPKEPVPLYMDGSLDAYAAFKSIIIKMAN
                     LNAFEATATEIPGAVTFLRGTQQCYVTINVAIDVEEEKKKLQEQIAYQQGFLISVDKK
                     LSNEKFVASAPAQVVEMEKKKRADAEEKIKMLEASLKNLN"
     gene            complement(5554..5706)
                     /locus_tag="PLB46_08855"
                     /pseudo
     CDS             complement(5554..5706)
                     /locus_tag="PLB46_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
BASE COUNT         1781 a         1154 c          966 g         1955 t
ORIGIN      
        1 atttgcaagt cattattgcg aaacacgccc agatataatg tgtatcagca aaggtattac
       61 aggaggcaca atggctttag gtgctacttt atgtgcgaca cctatttttg atgcatttta
      121 cagtgatgat aaaatgaagg cattatacca tggtcatagt tatactgcta atcctttagc
      181 ctgcacagcc gcaaatgcca gtctggacat cattgaacaa gcaggattta ttgataatgt
      241 aatcagtata acttctattc aaagcgattt tgctgagcga ttaaaaactt ttgttactgc
      301 aacaactaaa caggtaacca atattcgtca gcacggaacc attttagcct ttgatgtagt
      361 taccccacat caaacaggtt attttaatga actgcgcgat gaaatgtatc gattttttat
      421 tgggaatggc gtattgttgc gtccgttagg aaatacagtt tatatcatgc caccttattg
      481 cattaatgta gaacagatgg taaaaattca tgctgttatt ctggcatatc ttgcgcagcc
      541 ctgattaatc ccaattcggt ttttgtttat tgaggaagga attgatgcca tgtttacaat
      601 catcagtggc tcttgctttg gcattcattt cagcggcata atgtaatgct tcatttaatc
      661 ccatttcctg cacagcagca atcatttgtt tggtagcttg taatgaagca cgactcgttt
      721 tatcaattaa caattggcat aactgttcga cagttgcttc aatattttcc acaccaacaa
      781 catcattaat taaacccatt tgtttagctt ctgctgctga aattaatttc cctgtcaaca
      841 gtaattcttt tgcattacgt tctccaattt ttcgcaataa aaacaccatc acaattgccg
      901 gaataaatcc gataccaact tctgtgtatc cgaattttgc ttcatcaacg gcaaacgaaa
      961 aatcacagat agtagctaac ccacaaccac ctgctatagc atgtccttct atttgtgcaa
     1021 ttaccggttt agaaagcgta taaatttgtt tatacaattc catcaacgac aatgaatctt
     1081 ccagattttc ctgataggaa ttattttgta aggactgcag ataacctaaa tcggcgccgg
     1141 cgctaaatac ttcaccatta ccttttaata caataacttt cacttgcgta tcaccttctg
     1201 ccattgcaaa ggctgctttt aattctgcaa ccaatgtggc attaaaagca tttcttttat
     1261 ccgggcgatt tagtgtaatg ctacccactt tttgatgaac agcataagtt atggtactcc
     1321 aattcataat ggtatcgtcc tgcgtttaat taaaaatcga ttttataata gaacaaaggt
     1381 aaaaatccta actgatattc ctcttttata gggcttacag taatatctgc aggatttggt
     1441 gcataggtta accctaatac attttgcaca tcaaataaat tcaccaggtc gatagcaatt
     1501 tcatgagtta ccttgttttt ctttgaattg atgcggtaag ccaactttaa atcgaaacgg
     1561 aaataatctt tcagttgcaa agtattggtg agcgaatcga taaaaatcaa ttctttagca
     1621 gcgatagttg ccattgtgtc gacaggagtt gaaagtttac cacctgcata tgtcatttta
     1681 atgcccgttg taatgacatt cctgtcgcta atcgtcaatt catatccacc tagtaaattc
     1741 gctgcgtagg agccgttaaa atcggttttg cgttcaatac catctgaacc tacatataat
     1801 gaattgtata acgaacctgt agccaggaaa aagaaatgat tgctgaagaa tttttccagt
     1861 gttaattcca ctccataatt ttttcctgta ccggtatttt gcagtgaatc agggaaaaag
     1921 cgcgaaaagc cacttccttc attggctaat gaaaaagctg aaagttgtac ttcaacaggt
     1981 acatcgtata aatattggaa atatgtttcc attttcaggc ggaaattttt tcccaacatt
     2041 tgatcatatc ctaacacact atgaaaactt ctgataaaac ctacgtcttc attaattaat
     2101 ataaatgaat tatcgggttg tatttgctga taagtgtatt gataactcgg caacatttgg
     2161 ctatgtaaac ccgctccgaa attaagcgat gatttttcgg taagggtata acttaatccc
     2221 aatcgtggtt caattgattt actgtttgaa ttgatggtaa acagctgacc gtgtaaacca
     2281 ccgtttacca ctaacttttc tgcagctttg tatttccatt gaaaaaatgc ctgaaataac
     2341 aatgcattat cagtatagtc gtaacggttc tcaaaatccc aggtaaataa atttaaatta
     2401 gaatcaatat gattatacat atattgttcc gcgcttacac ctgctttaaa actatggcgg
     2461 ttatttactt tataattcaa gtgatataat gctgttattt tttggtcttt gtaactataa
     2521 ttcatgttgg gcaccatcga gtctatttca aataaagtat cgcggtacac taaaatatgg
     2581 tttgcatgat tttcttgcca tgaataagct acaactgtct ttccataagc ttttggattg
     2641 atttgcatgc cataagaaac acctgctacg gacatgctgg taccaaaata ttggtctcgg
     2701 tcgctatcgc catataattc agtttcagct ttattatatt cactaactaa aatatcgata
     2761 ttacttttcc caccaattgc aaacacatta attatacttg ttcctttggg aaaagataat
     2821 ttaacagcac catcaaaata attaggtact gctgatgttc caatactcaa attcataaac
     2881 tggaataatt gcaaagttga atatcggaaa ctggcaaggt aagatgattt attactttta
     2941 ttaataggcc cctcagcaaa taattccgta cctaaaaatc caagttgtcc gctaaattca
     3001 tgttgctcat tatttccacg gcgcatattt aaatcaaaaa cacctgcaat agaattacca
     3061 tattctgccg gaaaagcacc ggtaaaaaag tcggaattac taagatattt attattgata
     3121 atagaaacag gtccacctgt tgttccggca atggcgaaat ggttaggatt aggaatatcc
     3181 accccttcta aacgccaaag tacaccaatt ggcgaattac ctctaaccac aatatcgttg
     3241 cgagaatcgt ctgcaccttg cacacctgca aaattgcttg ccattcgagc gggatcgcct
     3301 ctacttcctg catagcggtc ggtttcttcc acactaaatg tgcgcacact aaccacagcc
     3361 atttcattat ttacttcccg tttatcctga taagctttga tttccagtcc ctccaataaa
     3421 atggtctgtt cttctaattc tacgttcaaa ataacttctt tacctcccgt aataatcaga
     3481 ttactaactg gtaaatcagt ataaccgaca cttctaaaaa ccatatttac gcgaccaacc
     3541 ggtacattag taactctaaa atcaccactt tcgtcactca ctcccccacc aaccattacc
     3601 gaatcagcat acaccaaaac ggtagcacca agaacaggga attttgaatc tttgtcactc
     3661 acgtgtccac ggatagtttg ggtaacattt tgagaaaaga ggaatagcgg aaaaaggaga
     3721 caaattaatg tgtaactaat tttcatgtta tgaattttcg ttaaaagtaa agctcccaaa
     3781 agggaaacgt tacggaattt tgaacatacg gcagtgcaag attattgatt ttcgcaaaaa
     3841 aaagaaaggc atccacaaaa gtgaatgcct taaatactag ttaaagtaag cttatttagt
     3901 tggagttacc ataccggtaa ttgcatcgca gcttgatttg caagcagcct gaataccagt
     3961 taaagattcg ttcatagcta ccagttggtc gttataagta gctaatttag cattccagtt
     4021 attatctagt tcagctttga attcagcatc agctaatttt tcttcctgtg cttttttctt
     4081 ccattctgca taagcagtag catctgcagc ccattgagtc atagcagcag aatagtcgcc
     4141 tttcattttt tccatttgac ctttaatcat cgtgcacata gtcaacatag agtctacact
     4201 agctttggtt gcaggtttta atttggacat atccattgca tcattagcag ccatcatggt
     4261 agtcatttca ccaaaagcag tcgtcatggt tgttcccatg gcagtcatat tgccttcagt
     4321 tgctttccaa tttgtttcaa atgtttgcat ggcagctttc gtttcttcgc ttaagccgcc
     4381 ttttttgcca catgaagtaa caaaaagtgc tgttgttatt gccattacgg caaggaatcc
     4441 ttttttcata atatgtattg gtttaggtta gtttaagatg caaaggtaaa ttgaataaat
     4501 ttagtttttg gtgatataaa acaccaaaat gctgaattga agcagaaatg atgttttaag
     4561 tgcgttatgc ataaccatta tttacacaaa tagtttgaac acacctgaca ttatctgcaa
     4621 aagtgttcct taattgaggt tttttaaact tgcttccagc atttttattt tttcttccgc
     4681 atcggcgcgt ttcttttttt ccatttccac tacctgtgct ggagcgctgg caacaaattt
     4741 ttcgttgctg agttttttat ctacactaat taaaaatcct tgctgataag ctatttgctc
     4801 ttgtaatttt ttcttctctt cttctacgtc aattgcaaca ttaatggtaa cataacattg
     4861 ttgagttcca cgtaaaaacg taacggcacc aggtatttct gttgctgttg cttcaaaagc
     4921 attgaggttc gccattttaa ttatgattga tttaaatgca gcatacgcat ctaaactacc
     4981 atccatatat aacggcacgg gttctttagg actcatatta ttttttgcac gaatatcacg
     5041 tactttagaa ataatatctt ttaccgcttc accggtttca attaaatttt cattggttga
     5101 accgccttta ggtaatcgcg ccataatgat atcatcaccg gcatttctat cttgtaataa
     5161 atggtataac tcttcagtta taaaaggcat ataaggatgt aataaaacga ggcattgttc
     5221 aaaaaattca atggttttat cataactatt tttgtcgatt ggtttaccaa attcgggttt
     5281 aacaaattcg agataccaag aacagaaatc gtcccaaata aaattataca atgtcattaa
     5341 cgcatcacta attctaaaat gagtaaagtg atcgtcaatt gttgcgatag tgtgatttaa
     5401 tttcgattca aaccattcaa ttgctgcggt attttcagta ttattttctc cggaaatttc
     5461 ccatccttta attaaacgta aaacgttcca gagtttgttg ttaaagtttc tgccctgttc
     5521 aatcagttta tcatcataaa taatatcatt accggcaggt gaacacaata aaattcccat
     5581 acgaattgca tcggcaccat attgcgcaat taaatcgagc gggtcaggtg aattacctaa
     5641 acttttgctc atttttctac gctgtttatc gcgaacaata cctgttaaat aaacatcgct
     5701 gaaaggtttt tcatttttat attcatctta gacaactcag gggcaatttt aattgtcttg
     5761 tttgcgtaac cttgtgtctt tgatctgaaa ccatccggct ttgatgaaag gaaaatttca
     5821 agttgccatc gcgctgctgt ttgttgtatt catctt
//
LOCUS       DAORKW010000196         5846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_529_length_5846_cov_3.833189, whole genome shotgun sequence.
ACCESSION   DAORKW010000196 DAORKW010000000
VERSION     DAORKW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_529_length_5846_cov_3.833189"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLB46_08860"
     CDS             <1..403
                     /locus_tag="PLB46_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76666.1"
                     /translation="YSLVIIGFFAMIFKYGNYILYETIYLINGNVVYTEIVSKEIINE
                     KYIYHFIIQGENEINYLSLPTYKEYNLEEIVKVRTVPLFEKVFLGEFVLVSYLLGILL
                     LVFGFVISTLSFWMITGIENKFTLRIKSSKG"
     gene            647..967
                     /locus_tag="PLB46_08865"
     CDS             647..967
                     /locus_tag="PLB46_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76667.1"
                     /translation="MKKMILPVGLIFLVQSCTLFCHLDDKAAYEYVKELNTSILKPSS
                     YLYPTIEEINMKKEGRYYIFNDTMLRIYPLTKVDTLFEHFYFKIGCEYGKPYIEDGWI
                     EQFE"
     gene            complement(1081..2127)
                     /gene="rsmH"
                     /locus_tag="PLB46_08870"
     CDS             complement(1081..2127)
                     /gene="rsmH"
                     /locus_tag="PLB46_08870"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRF76668.1"
                     /translation="MDEQPKHTRRIRYSGTHPKTFKEKYKELNPEQYAEEVEKILQQG
                     KTPAGMHRSICVQEILDFLQVQPGQVGLDATLGYGGHTLELLKCLTPGGHLFATDVDS
                     IELPKTIERLKNLGYGEEVLSVRKMNFAGIDQIVAEAGQLDFVLADLGVSSMQIDNPD
                     RGFSFKIEGPLDLRLNPYAGKTAAAYLKTVDVEELTDVFIANADEPHAAAIANAITTA
                     IKKGFPINTTTQLKNIITTTLTALPKRPSNDEIKKTCQRCFQALRIEINDEFTVLDKF
                     LSRLPDALKPGGRVAILSFHSGEDRRIKKAFQAFYRDGIFSEIALEPIRPSAQECNSN
                     SRAKSAKLRWGVMG"
     gene            complement(2154..2678)
                     /locus_tag="PLB46_08875"
     CDS             complement(2154..2678)
                     /locus_tag="PLB46_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF76669.1"
                     /translation="MSQLFIGKPDYQNEPDYCAYFFELVPAIGLLDALQQSLEQTLTF
                     ISSLTPEQWQFAYQTNKWTIAQVVRHIIDCERVYTYRAFRFSRLDDTPLAAFDENRYI
                     ANIAPHFLEKETLLKEYAYVRNATISLFSNMTTEMLDFKGKSGAVNYTTRGLGYMTVG
                     HNLHHITFIRNNYL"
     gene            complement(2720..3352)
                     /locus_tag="PLB46_08880"
     CDS             complement(2720..3352)
                     /locus_tag="PLB46_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRF76670.1"
                     /translation="MTKIKAIVFDLGKVIFDLSYEQVIAYWAEKANKDYQELLDGFVY
                     NDIFEQYERGELSDAAFRDAVRTIYHLPITDEEFDSGWNNIYLDIFDGINNLLRDLSA
                     QYHVVALSNTNHIHKLVWEVRYADTLQYFEKIFASHEMGCRKPEPQCFHMVLDYLNLA
                     PEATVFVDDHPANIEAANRIGLHTVLVTSFPQMQHDLMQILNKQENTIVE"
     gene            complement(3527..4969)
                     /locus_tag="PLB46_08885"
     CDS             complement(3527..4969)
                     /locus_tag="PLB46_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76671.1"
                     /translation="MDDLFVLVKSLSEADKKYFRQYASLQGGNANYLKLFNYIDTLDS
                     YTDEIIKTAFKGEAFVKQLSVAKNYLNEVILRTFRLSAESETAETALQLLMLDIRFLM
                     SKRCLSQVKKMLKRAQKLAEEQENFIILINIYALQRNLLTEFKFEARENISFDAIAEE
                     EENTIKKLINVNAIYNIYCKSKVLITSTRRTLNDSNKLRDFDALFNHPLMLEESTALS
                     NRAKHWFWSALHFKHVYNTDITKDLECCLQHLALFEREPGFANTRPLSYLTVVNNLLE
                     ACRATNNLALAEQHVGLLHQIKTHNKREADGQKVYIAHHTMWIALRKNDWHSAVAIAK
                     DEALRLEEQLMLKKDSVIVQYTMITIVFITAREWSLAGDMINKLLSIPKNGIREDILE
                     HAKLMQLILQVETQQFDIAAGLHRNIRRSVSPYPLVNFLLDYYRDYLKQPSELKHIKT
                     QYAELLQKNIIPPPEEFPEFTQMVASIINR"
     gene            5273..>5544
                     /locus_tag="PLB46_08890"
     CDS             5273..>5544
                     /locus_tag="PLB46_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76672.1"
                     /translation="MQHKPSPTKHLWLTLAAMFWLGNCFAGWSPDTIAVVEQPAVIDT
                     SVNNGIGAGINGGLQLSNVFYHTSLDTAYRSPLSYIISGQFNLSWK"
     assembly_gap    5545..5554
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5555..>5846
                     /locus_tag="PLB46_08895"
     CDS             <5555..>5846
                     /locus_tag="PLB46_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76673.1"
                     /translation="SLKITLPFSFILSEQERNFRQPFNQFGASPQYKWVTIHAGYRNI
                     TFSPYTLAGHTFLGGGVELNPGKLRFAAIYGRFTKAVHPDSVPLFSHGRASAR"
BASE COUNT         1760 a         1044 c         1102 g         1930 t
ORIGIN      
        1 gtattcacta gtaataattg gcttctttgc gatgattttc aaatatggca actatatact
       61 ctatgaaaca atttatttga taaatggaaa tgttgtatat acagaaattg tatccaaaga
      121 aataataaat gaaaaatata tttaccattt tataatacag ggcgaaaatg aaattaatta
      181 tttatcatta ccaacttata aggaatacaa cttggaagag attgttaaag taagaactgt
      241 tccattgttt gaaaaagtat tcttaggtga atttgtattg gtttcctact tgttaggaat
      301 attattgctc gttttcggct ttgtaatttc aactttatcg ttttggatga tcaccgggat
      361 tgaaaataag tttactttaa gaataaagag ttcaaaaggg taacaattgt tattttgctt
      421 tgtaagtata agcaatccga ataattaaaa aagttgaaag ttatgtttca gatcgactgc
      481 aaaaaacaga cttacttcta ttgtttgaaa attttcattt atttccgagc tgggttttgc
      541 cggatttgtt tatttttagg ggcggcaagc cccatgctag gactttgagt gaaaccatgc
      601 cacgaaaaaa ataattataa taactattga aataatcgat gtgcgtatga aaaaaatgat
      661 cttacctgtc gggttaatat ttttagtgca atcatgtaca ttgttctgcc atcttgacga
      721 taaggcagcc tatgaatatg taaaagaact taatacatcg atattaaagc caagcagcta
      781 tttatatcca acgatagaag aaataaatat gaaaaaagag ggtagatatt acatctttaa
      841 tgataccatg ttaagaattt accctttaac taaagtcgat actttatttg agcattttta
      901 ttttaaaata ggatgtgaat atggcaaacc ttatattgaa gatggctgga ttgaacaatt
      961 tgaataatac ctataaaagg tacatacata tgcgatttgc cagcatagta ttaatttacc
     1021 caacagttta taccccagca gaaaacaact gaatcatttt caaacacttt tacttccatc
     1081 ctaccccatc accccccatc tcaacttcgc tgatttagcg cgcgaattac tattacattc
     1141 ctgagctgat ggacggatag gttctaaggc tatttcacta aaaatgccat cacggtaaaa
     1201 ggcttgaaat gcttttttta tgcgacgatc ttcgccggag tggaaggata aaatggctac
     1261 tctcccaccc ggttttaaag catcgggaag tcgggataaa aatttatcga gcacggtaaa
     1321 ttcgtcgttg atttcgatgc gcaaagcctg gaaacaacgc tgacaagttt ttttaatttc
     1381 gtcgttggag gggcgtttgg gtaatgcggt taatgtggtg gtgataatat tttttaattg
     1441 ggttgtagta ttaattggaa aacctttttt tattgctgta gtaatagcat tggcaattgc
     1501 tgctgcatgt ggttcgtcgg cattggcgat gaatacatct gttaattctt ccacatcaac
     1561 tgtttttaaa taagcggctg ccgtttttcc ggcataagga tttaatcgca aatccaaagg
     1621 accttcaatt ttaaatgaaa atcctctgtc gggattatca atttgcatgg aagatacgcc
     1681 taaatcggct aacacaaaat cgagctgacc ggcttctgca actatttggt cgatgccggc
     1741 aaaattcatt tttcttacag acaacacttc ttctccgtac cctaaatttt ttaatcgctc
     1801 aatggttttt ggcaattcaa tactatctac atcggtggca aataaatgcc cgccgggcgt
     1861 caaacatttt aataattcca gtgtatgtcc gccataccct aaagtggcat ctaaccctac
     1921 ctgaccgggt tgcacctgca aaaaatctaa aatttcctgc acacaaattg agcgatgcat
     1981 acctgcaggt gttttccctt gttgcaatat tttttctact tcttcggcgt attgttcagg
     2041 atttaattcc ttgtattttt ccttaaaagt tttgggatgt gttccactat atcttattct
     2101 ccgggtatgt tttggttgct catccataag gtaaatttac gcgacaattt tatttacaga
     2161 taattatttc gaataaaagt gatatgatgc aggttatgcc caactgtcat gtatcccaaa
     2221 ccgcgtgtgg tataatttac agcaccggat ttgcctttaa aatcgagcat ctcggtggtc
     2281 atgttggaaa acagactaat agtagcattt cgaacatatg catattcttt taacagggtt
     2341 tccttttcca aaaaatgggg ggcaatatta gcgatatacc tattttcgtc aaaagcagcc
     2401 aaaggtgtat cgtctaatct ggaaaaacga aatgcacgat aagtgtatac tcgttcgcaa
     2461 tctattatat gcctaactac ctgtgcaatt gtccatttat ttgtttgata ggcaaattgc
     2521 cattgttccg gcgtaagcga ggaaataaat gtgagggttt gctccaaact ttgttgtaat
     2581 gcatcaagca atccgattgc aggaaccagc tcaaaaaaat aagcacaata atccggttca
     2641 ttttgataat ccggttttcc gataaataac tggctcattt tataaaataa tataaatagg
     2701 taaaaattaa ttacaacaat tattcaacaa tagtattttc ctgtttattt aatatctgca
     2761 ttaaatcatg ttgcatttgt ggaaatgaag tcaccaaaac ggtatgtaac ccgatgcgat
     2821 tagccgcttc aatattcgcc ggatgatcgt cgacaaaaac cgttgcttcc ggcgccaggt
     2881 ttaaataatc cagtaccatg tgaaaacatt gtggttcagg tttgcggcaa cccatttcgt
     2941 gtgaagcaaa tattttttca aaatattgta aggtgtcggc ataacgcact tcccatacca
     3001 atttatgaat atgattggta tttgaaagtg ctacaacatg atattgagcg gacaaatcac
     3061 gcaataaatt attgatacca tcaaaaatat ccaggtaaat attattccaa ccggaatcaa
     3121 actcttcgtc tgtaataggt aaatgatata ttgtgcgcac ggcatcacga aaagcagcat
     3181 cgcttaattc accgcgttca tattgttcaa aaatgtcgtt gtaaacaaac ccatccaata
     3241 attcctgata atctttattc gctttttcgg cccaataggc tatcacctgc tcgtaggaca
     3301 ggtcgaatat tactttgcct aaatcaaaaa ctattgcttt tattttcgtc attgctaata
     3361 ttggaattag acgacaaatt aacggctttt tgagggatta ggcagcttaa attgtcccca
     3421 tgggaattat atgtcacatt tttttaccaa aagtacaata ttatcaatac ttacagacta
     3481 ttaaatgcca atatttagcc taattgcctg tcacatttaa ataacatcac ctattaatta
     3541 tagaagcaac catttgggta aattcaggga attcctcggg aggaggaata atattttttt
     3601 gcaggagttc ggcgtattgt gttttaatat gttttagttc ggaaggttgt tttaaatagt
     3661 cgcgataata atcgagtaaa aagttcacta gcggataagg agaaacggag cggcgaatat
     3721 tgcgatgaag tccggctgca atatcaaatt gttgtgtttc cacttgtaaa atcaattgca
     3781 tgagtttagc atgctcgaga atatcttctc gtataccatt tttaggaatg gataataatt
     3841 tatttatcat atcacctgcc aatgaccatt ctcttgctgt gataaataca atggtaatca
     3901 tagtatattg aactatcaca ctatcttttt tgagcattag ctgctcttct aaacgaaggg
     3961 cttcatcttt tgcaatagcg actgcactat gccaatcgtt tttacggagt gcaatccaca
     4021 tggtatgatg tgcaatgtaa actttttgcc cgtctgcttc gcgtttgtta tgtgttttta
     4081 tttgatgcag caatcccaca tgttgctcag ctagcgcaag gttattagtt gctctgcaag
     4141 cttccaataa attatttacc actgtcaaat aactcaacgg acgagtattg gcaaaacccg
     4201 gttcacgttc aaacaaagcc aggtgttgca aacaacactc caggtctttt gttatatcgg
     4261 tattataaac atgtttaaaa tgtaaagcac tccaaaacca gtgtttagct ctattgctta
     4321 atgcagtgct ttcttctaac atcaggggat gattaaataa cgcatcaaaa tcacgtaatt
     4381 tattgctatc atttaatgtt ctccgagttg acgtaatgag cactttagat ttgcaataaa
     4441 tattataaat ggcatttaca ttaattagtt tttttatggt gttttcttcc tcttcggcaa
     4501 tggcatcaaa agaaatattt tctctggctt caaatttaaa ttcggtgagc agattgcgct
     4561 gcagtgcata aatattaatt aatataataa aattttcctg ttcttctgcc agtttttgcg
     4621 cgcgtttcaa cattttttta acctgcgata aacaccgttt gctcatgaga aaacgaatgt
     4681 cgagcataag gagttgtaat gctgtttcag cggtttcgga ttcggcactt aaacggaatg
     4741 tgcgcagtat aacttcgttt aagtaatttt tggctacaga taattgttta acaaaagcct
     4801 cacctttaaa tgctgttttt attatttcat ctgtatagga atcgagggta tcgatataat
     4861 taaatagttt caaataattg gcgttaccac cctgtagcga agcatattgc cggaaatatt
     4921 ttttatctgc ttccgaaagc gattttacta atacaaacag gtcgtccata atatcaggct
     4981 aagataagga atctaagttt tcagattttt atggagcggt gaaaattaat tggctcttac
     5041 aaatcgcgtt aggggttgca acggaaaccc cgcaatccgc cttttcggcg gaagaggagt
     5101 tggagtggaa aacccggtgc cgcacgaaaa tgttgtgagg attaccatta atattgcggc
     5161 aaacgcccac atttttttca aatttatcga gctcccccta ttgtggtatt ttatgcggtg
     5221 gctgttcatt ggaattttga tatagttaaa gtgtattatc aaaattctgt ttatgcaaca
     5281 caaaccctca cccactaagc atttgtggct tacgcttgca gcaatgtttt ggcttggcaa
     5341 ttgttttgcc ggttggagtc ctgacacaat tgcggtagtg gagcagcctg cagtcattga
     5401 cacgagtgtg aacaatggca ttggtgcggg cattaacggg ggattgcaat tgagtaatgt
     5461 tttttatcat acttcgctgg acacggctta tcgttcgccg ctatcgtata ttatttcggg
     5521 gcaatttaac ttgagttgga aaaannnnnn nnnnctcact taaaattact ttaccattca
     5581 gttttatttt aagtgagcag gaaagaaatt ttcgtcagcc atttaatcag tttggtgcta
     5641 gtccgcaata caaatgggta accattcacg cgggttatcg caatattact ttttcgccat
     5701 atacattggc gggacatact tttttaggtg gtggggttga attaaatccg ggtaagttgc
     5761 gttttgctgc aatttatggt cgtttcacca aagcggtgca tccggatagt gtgcccctat
     5821 tctcacacgg gagagcgtcg gcgcgg
//
LOCUS       DAORKW010000197         5840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_532_length_5840_cov_6.313570, whole genome shotgun sequence.
ACCESSION   DAORKW010000197 DAORKW010000000
VERSION     DAORKW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_532_length_5840_cov_6.313570"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   390..1346
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=390..435
                     /rpt_unit_seq="gttgtgaattgatttcaaattatgttcttatttttggttcgac
                     aac"
     gene            complement(1458..1763)
                     /gene="cas2"
                     /locus_tag="PLB46_08900"
     CDS             complement(1458..1763)
                     /gene="cas2"
                     /locus_tag="PLB46_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRF76674.1"
                     /translation="MWILVFFDLPTETANDRKIYTGFRKSLLKDGFYMFQFSIYVRHC
                     PSKENMEVHLKRVKKNLPANGHVGLLAITDRQFGDMEIYFGKEMAPSLPIPQQLELF"
     gene            complement(1798..2709)
                     /gene="cas1"
                     /locus_tag="PLB46_08905"
     CDS             complement(1798..2709)
                     /gene="cas1"
                     /locus_tag="PLB46_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HRF76675.1"
                     /translation="MAKRTLFIVNPAKLSTRLEQLIVEQKNISNELEVRQVPIEDISI
                     IILESLQITVTTAAINKLMENNVVVVSCNEKLIPNGLLIPIEGNTLLQERYRLQFAAS
                     EPLKKKLWQQTVEAKIKNQAAVLDYLGIENNKLKIMSNDVMSGDAGNIEGAAAAYYFD
                     KIFTEFLIKFKRSREGDAPNNLLNYGYAILRANVTREIVAAGLLPALGLHHRNKYNAY
                     CLADDIMEPFRPFVDVCVIDILKGETNVSLLTKELKSQLIMVTSKDAIYKKEICVLAT
                     AIDKVCSNLVKCYSGEVRKLNYPLLKL"
     gene            complement(2713..>5840)
                     /gene="cas9"
                     /locus_tag="PLB46_08910"
     CDS             complement(2713..>5840)
                     /gene="cas9"
                     /locus_tag="PLB46_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HRF76676.1"
                     /translation="QRPLKSQKGLVSVCEFEGFYTKNKEGKVLFTGPKVAPRSSPLFQ
                     VCKLWESINAITIIKKGTGLKRAPKLDISPFKNQLYEHLNYNGDLKEKKLFEILKISK
                     SEGYEADRKIATKGIQGNLTIQSFKKALGVDGTDELLRFNLNITKSTTVDKLTGEVRT
                     IDTISPGIETEPLFKLWHICYSIADVDEKRNVLKRNFDFSIEQINNLLGIDFKKSGYG
                     NKSAKAMRKILPHLMNGMVYSDSMEASGIKHSDSLSTEENVIRKLNDTIPIISKNDLR
                     QPVVEKILNQMIHQVNTIIKTYGRPDIIRVELARELKQSKEERYKFFTAMNLRTKQAE
                     KIALELESRYHVKANGRNIEKWRLWHEVNGTCIYCGKQISLTQFLNGIEADVEHIIPK
                     SVLFDNSFANKTISHVECNKAKGNKTAYDYMLSLGEDHTQRFLKQVELLFFKPVSGEK
                     ETHEGAHCTVGKISKNKYNRLQWSLEDIPKDFILRQLQETRYISKKAKEILNTVCYDV
                     YSTTGAITEKLRNIWGWNESLENIHIKKFSQVDGLTHEIYNNETGEKRTKIIGWTKRN
                     DHRHHALDALVIACTDQSLIQKINTLSAQSTKDYLNGEVQNLQFSQRLSLLDKFILSK
                     RPFLTKEVAERLENILVSYKPGNKLTTYSTRLVKINGEKLKAQTNIITPRGSLSEASV
                     YGRIKVIDRDSNTLMPIKYPVKYLLQHSEKICSPKIKALIEERLIEFNYDVKGAIKSL
                     KNNPFRFNNMELHYGYCYEEMFVIKYPLNQNFDKVDKIVDLRVRECVQKRLDSYEGDP
                     RKAFKNLEEQPLYLDTKETIRINSVRCRAALQFAESIPVLDDNNKTLYTKYVKTGNNH
                     HVGIYETPVGDLVAVPSDFWHGVQRVLFFNNNFVKEIREKFIKNAIVFDPKIMWDSIL
                     ELKKDILDEDFLKKLPADNLKLLCTLQQNDMYVFGLPLLELEQAITENNYKLIQSRLY
                     RVNVISKTSINYQFRLHSETKVDDKYNGKSSEMTSKKLGVLIIVQSLDKWRELQPIKV
                     SINRLGKITIA"
BASE COUNT         1632 a         1015 c          932 g         2261 t
ORIGIN      
        1 taaccgttgg ttgtgaattg atttcaaatt atgttcttat ttttggttcg acaacaattt
       61 tttggaggca tagttgtaac cgttggttgt gaattgattt caaattatgt tcttattttt
      121 ggttcgacaa cttcatatcc ggagtttaat gcacaggtag tgttgtgaat tgatttcaaa
      181 ttatgttctt atttttggtt cgacaacttc ttcattactc attaaccttt caaaatagtt
      241 gtgaattgat ttcaaattat gttcttattt ttggttcgac aacgttaatc aatagcccaa
      301 aatgcttgca atagttgtga attgatttca aattatgttc ttatttttgg ttcgacaact
      361 cagaaataat ccgaacatcg ttgctaatgg ttgtgaattg atttcaaatt atgttcttat
      421 ttttggttcg acaactgcat tgcatggatt gctaatcctg accagttgtg aattgatttc
      481 aaattatgtt cttatttttg gttcgacaac acatattggc atagttccaa tttatgatac
      541 gttgtgaatt gatttcaaat tatgttctta tttttggttc gacaacgcca atattgaaac
      601 atggcgtcag ctccgggttg tgaattgatt tcaaattatg ttcttatttt tggttcgaca
      661 actgacatat cgttttccgg aattgaagat atgttgtgaa ttgatttcaa attatgttct
      721 tatttttggt tcgacaacaa ttggcatcaa tggcgaccgt ggtgcaaagt tgtgaattga
      781 tttcaaatta tgttcttatt tttggttcga caacccgcct gcaggtattc atcgttggac
      841 gttagttgtg aattgatttc aaattatgtt cttatttttg gttcgacaac ccggtattaa
      901 aagaaattga tgctgcagtt gttgtgaatt gatttcaaat tatgttctta tttttggttc
      961 gacaactctg tgcatttaca ttccggacag gctttagttg tgaattgatt tcaaattatg
     1021 ttcttatttt tggttcgaca acaaggtatt aagtttatta agtgttcgtt tagttgtgaa
     1081 ttgatttcaa attatgttct tatttttggt tcgacaactt tgaacctttg ccatagtgat
     1141 acaaaggtgt tgtgaattga tttcaaatta tgttcttatt tttggttcga caactatttc
     1201 aacgatcggg atgcattcaa agctgttgtg aattgatttc aaattatgtt cttatttttg
     1261 gttcgacaac atccgaacta tcctgcatct gatgctggaa gttgtgaatt gatttcaaat
     1321 tatgttctta tttttggttc gacaaccgcg cctgctagaa acccttattt taaagggttt
     1381 ctagcagttt ttaggatatt ttttttgagg gtggaactta atccggacat ttaattttga
     1441 ggtttttggg gggtaggtta aaacaactcc aattgttgtg gtatgggtaa agaaggagcc
     1501 atttctttgc caaaatatat ctccatatcg ccaaactgcc tatctgtaat agctagcaag
     1561 ccaacatggc catttgcggg taaatttttt ttcactcgtt ttaagtgcac ctccatgttc
     1621 tccttagatg ggcagtgtct aacgtaaatg ctgaattgaa acatgtagaa tccatctttt
     1681 aataaacttt ttctgaatcc ggtataaatt tttctgtcat tagcggtttc agttggcaaa
     1741 tcaaaaaata ctagtatcca catgattcgg tatttattta atcggctcat ggaaaattta
     1801 taatttcaag agtgggtagt tcaattttct tacctctccg gaatagcact taacaagatt
     1861 ggagcagacc ttatcgattg ctgtagctaa aacacaaatt tccttcttgt aaatagcatc
     1921 tttgcttgtc accatgatta actggctttt gagttccttt gtgagcaagg aaacatttgt
     1981 ttcacctttt aaaatgtcta ttacgcaaac atcaacaaat ggtcgaaaag gttccataat
     2041 atcatcagca agacaatagg cgttgtattt atttctatgg tgcaaaccca acgccggcaa
     2101 taaaccagca gcaactattt ctctcgtcac atttgcgcgt aaaatggcat agccataatt
     2161 taatagatta tttggggcgt cgccctccct tgatcgttta aattttatta aaaattcagt
     2221 gaaaattttg tcaaaataat atgccgcagc agcaccttca atattacctg catctccact
     2281 cattacatcg tttgacataa tttttaactt attattttct attcctaaat aatccaaaac
     2341 agcggcttgg ttttttattt ttgcttctac cgtttgctgc caaagcttct ttttaagtgg
     2401 ttctgatgca gcaaattgca gccggtaacg ttcctgcagt agcgtattac cctctattgg
     2461 aattaacaaa ccattaggaa ttagcttttc gttgcaactc acaactacta cattattttc
     2521 cattaattta tttattgcag cagtggtaac cgtaatttga agactttcta atattatgat
     2581 agaaatatct tcaataggaa cctgacggac ttctagttcg tttgaaatat ttttttgttc
     2641 aacaattaat tgttctaaac gggtagatag tttagctgga ttgacaataa acaaagtacg
     2701 tttcgccata tattatgcaa ttgtaatttt accaagtcta ttgatagata cttttattgg
     2761 ttgtaattct ctccatttat ctaaactttg tacaataatt aaaacaccca atttcttgga
     2821 agtcatttca ctcgattttc cattatattt atcgtctacc tttgtttctg agtgtaatct
     2881 aaattgataa tttattgatg ttttagaaat aacattgaca cgatacaatc ttgattgtat
     2941 tagtttataa ttgttttcgg taattgcctg ctcaagctct aataaaggca agccgaaaac
     3001 atacatatca ttttgctgta aagtacataa taatttcaag ttgtccgctg gtagcttttt
     3061 caaaaaatct tcatctaaaa tatctttttt taactcaaga attgaatccc acattatttt
     3121 agggtcaaac acaatcgcat tttttatgaa cttttccctt atttccttca cgaaattatt
     3181 attgaaaaat aaaacgcgtt gcacgccatg ccaaaagtcg gatggcactg ccaccaaatc
     3241 gcctactgga gtttcgtaaa taccaacatg gtggttgttt cccgttttca catatttagt
     3301 gtacagcgtt ttgttgttat cgtccaatac aggaatagat tcggcaaact gtagagctgc
     3361 cctgcagcga acagagttaa ttctaattgt ttcttttgta tctaaataca aaggctgctc
     3421 ttccaagttt ttaaaagcct ttctagggtc gccttcatat gagtccaacc tcttttgaac
     3481 acattctctt actcttaggt ctacgatttt gtcaactttg tcgaaatttt ggtttagtgg
     3541 atacttaatt acaaacattt cctcgtaaca atacccgtaa tgtagttcca tattattaaa
     3601 cctaaaaggg ttatttttta gagattttat cgctcctttt acatcataat taaattctat
     3661 taacctttct tcaattaatg ccttaatttt aggactacaa attttttctg aatgttgtaa
     3721 taaatattta actggatact ttattggcat caaagtgttt gaatctctat caatcacttt
     3781 tattcttcca taaacactag cctcagataa agagcctcga ggagtaataa tattcgtttg
     3841 tgctttaagt ttttcaccat ttattttcac caatcgtgtg ctatatgtgg ttaatttgtt
     3901 tcctggttta taagagacca atatattttc caacctttct gcaacttctt tcgttagaaa
     3961 tgggcgtttg ctcaaaataa atttatccaa aagactcaag cgctgcgaaa attgcaagtt
     4021 ttgcacttct ccatttaaat aatcttttgt ggattgagcg cttaaagtat taattttttg
     4081 tattaaggac tggtcagtac atgcaataac aagtgcatcg agcgcatgat ggcggtggtc
     4141 atttctttta gtccatccaa ttatttttgt tcttttctca cctgtttcat tgttatatat
     4201 ttcatgtgtt aatccatcta cttgggaaaa cttttttata tgaatatttt ctaacgactc
     4261 gttccaaccc caaatatttc ttaatttttc tgttattgct ccagttgtac tatacacgtc
     4321 ataacaaaca gtgtttaaaa tttctttggc ttttttacta atatatcgtg tttcttgtaa
     4381 ttgtcgtaaa ataaaatctt taggtatgtc ttctaacgac cattgtaggc gattgtattt
     4441 gtttttactg attttaccta cagtgcagtg agcgccttcg tgggtttctt tttcccccga
     4501 tactggtttg aaaaataaca gctcaacttg ttttaaaaac ctttgtgtat ggtcctcacc
     4561 taaacttaac atataatcat aagcggtttt atttcctttt gctttattgc actcaacatg
     4621 tgatatggtt ttattcgcaa acgagttgtc aaataaaaca cttttaggaa taatatgctc
     4681 aacatctgct tcaatgccat ttaaaaattg tgtcaaggaa atttgtttgc cgcaataaat
     4741 acatgttcca tttacctcat gccaaagcct ccatttttca atatttcttc catttgcttt
     4801 tacatgatat cttgattcca attccaaagc aattttttca gcttgttttg tcctaagatt
     4861 cattgctgta aaaaatttat aacgctcttc tttactttgt tttaactctc tggccagctc
     4921 cactctaata atatcaggcc ttccataagt tttaattata gtattgacct ggtgaatcat
     4981 ttgattgaga attttctcaa caactggctg ccgtagatcg ttttttgaga taattggtat
     5041 tgtatcgttt aattttcgga ttacattttc ctccgttgat aatgaatcag aatgtttgat
     5101 accggaagcc tccattgaat cactgtaaac cattccgttc attagatgag gtaaaatctt
     5161 cctcattgct ttggccgatt tattgccata tccggatttt ttaaagtcta taccaagcag
     5221 gttattaatt tgttcaatag aaaaatcaaa atttcttttt aatacattac gtttttcgtc
     5281 aacatcagct attgaataac aaatgtgcca aagcttaaat aagggttctg tttcaattcc
     5341 gggagaaatc gtatctattg ttcttacctc tcctgtcaat ttgtcaactg ttgtggattt
     5401 tgtgatattt aaattaaacc gcaaaagttc atctgtgcca tcaacaccta atgccttttt
     5461 aaaagattgt atagttaaat ttccctgaat tcctttggtg gcaatttttc tatctgcttc
     5521 ataaccttct gatttggaaa tctttaggat ttcaaataat tttttttctt ttaagtcacc
     5581 attgtaattt aaatgctcgt atagttgatt tttaaatgga gatatgtcta attttggtgc
     5641 tctttttaaa cctgttccct tttttattat agtaatagca tttattgact cccaaagctt
     5701 acatacttgg aacaaagggg aacttctagg tgcaactttc ggtccggtaa acaacacctt
     5761 gccctcttta ttttttgtat aaaaaccctc aaactcgcaa acagaaacaa gtcccttttg
     5821 tgactttagt ggccgctgat
//
LOCUS       DAORKW010000198         5757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_548_length_5757_cov_4.140477, whole genome shotgun sequence.
ACCESSION   DAORKW010000198 DAORKW010000000
VERSION     DAORKW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_548_length_5757_cov_4.140477"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLB46_08915"
     CDS             <1..647
                     /locus_tag="PLB46_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF76677.1"
                     /translation="FYIGAPADGDSVFTIGAVDLNGNFASFSSHGPTFDGRIKPNVVG
                     QGAGTGVVTSNGTVAYGNGTSYSSPLIAGACASFMSAFPELSNMEVMDIIQSTASQAD
                     NPDNSMGYGIPNFAKAYMELKGIDISTENIISILPNPASDEVTIFINGQETGEASLYI
                     YDITGKRVYETTVTLWDGKLAPVYINGLNALASGMYTVYLAGDVYQETQKMIVR"
     gene            707..1273
                     /locus_tag="PLB46_08920"
     CDS             707..1273
                     /locus_tag="PLB46_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF76678.1"
                     /translation="MLDLNFNPFPILTTQRLVLRQITIKDAAALYALRSNETVMQYID
                     KPLAKSIADAVELIEKTMDALTYNEGITWAICLADDLTMIGTIGYWKFRKEHYRAEIG
                     YLLLPEFHGKGIMFEALQTIIRFGFQQLHLHSIEAIVNPDNVASIGILEKNGFIREAY
                     YKEDYYFEGKFLDSAVYSLLEPKFISLV"
     gene            1270..1593
                     /locus_tag="PLB46_08925"
     CDS             1270..1593
                     /locus_tag="PLB46_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRF76679.1"
                     /translation="MITPEVFRTLIFKLPDVVEVPHFDRAAFKANNRIFVSLAPDGLS
                     INIKFTLVDQSVFCAFDPTIIYPVPNKWGLQGWTTINLTKVKQSTLKDALKTAYNAFL
                     QLKKK"
     gene            1734..4088
                     /locus_tag="PLB46_08930"
     CDS             1734..4088
                     /locus_tag="PLB46_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF76680.1"
                     /translation="MKKALLLFWMLTCSILVFAQSKYTLSGYIKDGSSGEDLIGANVY
                     VKTDQVIGTATNVYGFFSMTLPAGIYDVTFSYIGYEDQVKTIDLNSDQRINVSLKQGE
                     ILEEVVIKEERNDANIQSTDMGTVELNIEQIKTIPVLFGEVDILKTIQLLPGVGGAGE
                     GNTGFYVRGGGPDQNLILLDEAVVYNTGHLFGFFSVFNSDAIKNVTLIKGGMPAQYGG
                     RLSSVVDVQMKEGNNQQYHAEGGIGLIASRLTIQGPIVKDKSSFIVSGRRTYIDAIVT
                     PFLKGTEFEGNGYYFYDLNTKVNYKFDDKNRIFLSGYFGQDVFNYVAPDNTLNISVPW
                     GNATATARWNHLFSDQLFMNVSAIYNSFDFSVKSEFQDFNFNLFSGVKDWNGKVDFDY
                     YPGANHAIKFGANYIFHTFTPYSAEGNFDDVEFGTDTLSNKLAHEAGIYIQDEFDVSK
                     RLKINVGVRGSYFAQVGPYNDIIYGDDNSTIVDTVSYENGEKIADYTALEPRFNARFI
                     VDPQSSIKAGITFTNQFIHLVSSSTSTLPTDLWIPSSAKVKPQIGVQYSIGYFRNFFD
                     NKYETSVEIYYKDLKNQIEYADGYVPELGSDIEDNFVFGRGRSYGAEFFIKKAKGKFN
                     GWIGYTLSKTERQFDNLSTGTAWFPTKYDRIHDLEVVAVYQLSERWDLSSTFVYATGQ
                     ATTIVESFYVIGGQLQTDYGPRNGYRMPAYHRCDVSATLKNKPGKKYESSWNFSIYNV
                     YSRKNPYFIYTTWEGDFAYGAVDVQAKQVSIFPIIPSVTWNFKF"
     gene            4111..5067
                     /locus_tag="PLB46_08935"
     CDS             4111..5067
                     /locus_tag="PLB46_08935"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HRF76681.1"
                     /translation="MKQSNIYFSLITAATISLMLGSCTTDITLDIPAPEQKIVVEGTI
                     EPGLPPIISLTSTVPFYGEVNFNELDNFYVHDAQVTVSDGVNTVELVEYCLNDLPEEL
                     LPLVAEFLGVDLDTAGGFAVNVCLYTVPDIFTGFPEFVGEVGKTYTLNINYEDQVLTS
                     STTMFPPVPPDSIYWEPHGNPENDTLVRLYIALSEPDPQGNFYRYFTRQNSEPFYSGY
                     SSVFDDNLINGQSFKFTVDRGFNPSEEFNQDTYGYFWKGDTIILKWCSIDFPTYDFWR
                     TLEFDSGTDGPFSSATIAQTNIIGGLGIWCAYGAAYDTLIVE"
     gene            5164..>5757
                     /locus_tag="PLB46_08940"
     CDS             5164..>5757
                     /locus_tag="PLB46_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF76682.1"
                     /translation="MQKLLAALILTICVSRGYAQTSKGDWLTGGAVYFSTSSSEGITL
                     TDTLITSSVNFNASAFGGYFVMDNLCVGLGLSYGRNAATNNYAPIELTDVSTSLSVEA
                     ITRYYYWNGDHVKLWTGLGVRKGFGKYDDHFYEYDDQGNGKEANQVDDIATFSIGFGP
                     GASIMLNPKLALEVYFGSIGYSTYTLEREDGSYDYTTN"
BASE COUNT         1754 a          926 c         1193 g         1884 t
ORIGIN      
        1 ggttttatat tggtgctcca gccgatggtg atagtgtatt tacaattggc gcagtcgatt
       61 taaatggcaa ttttgccagt tttagttcac atggacctac ttttgatggt agaataaaac
      121 ctaatgtggt gggacaggga gcaggaacag gtgttgttac ttcaaatggc actgttgctt
      181 atggcaatgg cacttcttat tcatctccgt taattgccgg agcttgtgcc agttttatgt
      241 ccgcatttcc tgaattatct aacatggaag tcatggatat cattcaatcc actgcatcac
      301 aagctgataa tccggataat tcgatgggtt acggtattcc taattttgcg aaagcatata
      361 tggaattaaa agggattgat atttcaacag aaaatattat ttctatttta cctaatcctg
      421 caagtgatga agtaaccatt tttataaatg ggcaggagac cggtgaagca tcactttata
      481 tttatgatat aaccggtaaa cgtgtttatg aaactacggt tacgttatgg gatggcaaac
      541 ttgctcccgt atatattaac ggattaaatg cactggcaag tggtatgtat actgtttatt
      601 tagccggtga tgtttatcag gaaacacaaa aaatgattgt cagataaatt acctttgtag
      661 cctattcagt aattgagtgt gattgatatt tgctgaatga gggcatatgc tagatctcaa
      721 ttttaatcct tttccgattt taacaacaca gcgtttagtg ctgaggcaaa ttactattaa
      781 agatgccgca gctttatatg cattacgttc taacgaaacg gtgatgcaat atattgacaa
      841 gccgcttgca aaatcgatag ctgatgcagt agagctcatt gaaaaaacga tggatgcact
      901 tacttacaat gaaggcatta catgggcaat ttgtctggca gatgatttaa cgatgattgg
      961 cacgattggg tattggaaat ttcgcaaaga gcattatcga gcagagattg ggtatttgtt
     1021 gttacccgaa tttcatggta aaggaatcat gtttgaagct ttacaaacta ttatccggtt
     1081 tggttttcaa caattgcatt tgcactcgat tgaggctatt gttaatccgg ataatgttgc
     1141 atcaatcggt attctcgaaa aaaacgggtt tattcgtgag gcatattata aagaagacta
     1201 ttattttgaa ggcaagtttt tagattccgc agtatattcg ttactggaac ctaaatttat
     1261 cagtttggta tgattactcc agaagttttc agaacactca tttttaaact gccggatgtc
     1321 gtggaagtgc cgcatttcga ccgtgctgct ttcaaagcga acaatcggat ttttgtttca
     1381 ttagctccgg atggcctttc tatcaatatt aaatttacgt tagtggatca gtctgttttt
     1441 tgtgcatttg accccactat catctacccg gtgcccaata aatggggctt acaaggctgg
     1501 actaccatca acttgactaa ggttaaacaa tctactttaa aagatgcact taaaactgca
     1561 tacaacgcgt ttttacaatt aaagaaaaaa taattgtaac aattagtggg tcatggcgtt
     1621 atatacatgg ttcggaacat tgttccgttc atagaagttt atatcccgtt catcgaaaat
     1681 atttcgttgt acggtaaact catttttact ttgcattgtt attatcacaa taaatgaaaa
     1741 aggcgctact cttgttctgg atgttgacgt gttccatcct tgtttttgca caatccaaat
     1801 acaccttaag cgggtatatc aaagatggtt catcgggtga ggatctgatt ggcgccaatg
     1861 tttatgtcaa aaccgaccag gttattggca ctgcaaccaa tgtttatggg ttcttttcta
     1921 tgaccctgcc agcaggcatt tatgatgtta cattttctta tatcggctat gaagaccagg
     1981 ttaaaacaat agatttaaac agcgaccaac gtatcaatgt ctctttaaaa cagggggaaa
     2041 ttctggaaga agttgttatt aaagaggagc ggaatgatgc caatattcaa agtaccgaca
     2101 tgggaactgt agaattaaat attgagcaaa taaaaacaat tcccgtatta tttggtgagg
     2161 ttgatatttt aaaaaccatt cagttattac cgggtgttgg cggcgcaggt gaaggtaata
     2221 ccggttttta tgtgagaggt ggcggccccg accaaaacct gattttattg gatgaagctg
     2281 ttgtatataa caccggccac ttatttggat tttttagtgt gtttaattct gatgctatta
     2341 aaaatgtaac acttattaaa ggtggaatgc cggctcaata cggaggcaga ttaagcagcg
     2401 tggtggatgt gcaaatgaaa gaaggcaata atcagcaata tcatgcagag ggcggtattg
     2461 ggttaattgc atctcgctta accattcagg ggccaattgt aaaagataaa tcgagtttta
     2521 tagtttcagg aagaagaact tatattgatg ccattgttac accattttta aaaggaacag
     2581 agtttgaagg caatggttat tatttttatg atttaaatac caaagttaat tataaattcg
     2641 acgataaaaa caggatattt ttaagtggtt attttggtca ggatgtattt aattatgtag
     2701 ctcccgataa tacactgaat attagtgtgc cttggggtaa tgcaactgct accgcgcgct
     2761 ggaaccattt attcagtgat cagctgttta tgaatgtgag tgctatttac aacagttttg
     2821 atttttcggt aaaaagcgaa tttcaggatt ttaattttaa tttattttca ggcgttaagg
     2881 attggaatgg caaagtggat tttgattatt atcccggcgc taatcacgcg attaagtttg
     2941 gtgcgaatta tatcttccat acatttactc cgtattctgc agaaggtaat tttgatgacg
     3001 tagaatttgg tacggacaca ttaagtaata aactagctca tgaagctggt atttatattc
     3061 aggatgaatt tgatgtctct aaacgcttga aaataaatgt tggggtaaga ggaagttatt
     3121 tcgctcaggt tggaccctac aatgatatta tttatggtga tgataatagc actattgtgg
     3181 atacggtgag ttatgaaaat ggtgaaaaaa ttgcggatta tacggccttg gaacccaggt
     3241 ttaatgcgcg ttttattgtt gacccacaaa gttcaataaa agccggtatt accttcacca
     3301 atcaatttat tcacttagtg agtagttcca cttcaacttt gcctaccgat ttatggatac
     3361 caagttcggc aaaggtaaaa cctcaaattg gcgtacaata ttcaattgga tatttcagaa
     3421 actttttcga taataaatat gaaacaagtg tagagattta ttacaaagac ttaaaaaatc
     3481 aaattgaata tgccgatggt tatgtacccg aattaggcag tgatattgaa gataattttg
     3541 ttttcggaag aggaagaagt tacggtgcag aattttttat taaaaaagcc aaaggaaaat
     3601 ttaatggatg gattggatat acattatcca aaacagaaag acaatttgat aatttatcta
     3661 caggaacggc ttggtttccg acaaaatatg accgcattca cgatttagaa gtagttgcag
     3721 tgtatcaatt aagtgagcgt tgggatttat cctcaacatt tgtttatgct accggtcagg
     3781 ctaccactat tgttgaaagt ttttatgtaa taggagggca gttgcaaaca gattacggtc
     3841 caagaaatgg atacagaatg ccggcctatc accgttgtga tgtttccgct acgctaaaaa
     3901 ataaacccgg aaaaaaatat gagagcagct ggaatttttc catatacaat gtgtattccc
     3961 gaaaaaatcc atattttatt tataccactt gggaaggcga ttttgcatac ggagcagtag
     4021 atgttcaggc taaacaggtg tctattttcc caattattcc gagtgtaacc tggaacttta
     4081 aattttaatt gtccaaataa gaaacataaa atgaaacaat caaatatata tttttcgttg
     4141 ataaccgctg caactatctc attaatgttg ggttcgtgca caacagatat cacgctggac
     4201 attccggcac cggaacaaaa aattgttgta gaaggaacca ttgaacctgg attacctccc
     4261 attatttcat taacgtctac tgttcctttt tacggagaag tgaattttaa tgaattagat
     4321 aatttttatg ttcacgatgc acaggtaact gtgagcgatg gagtaaatac ggttgaactt
     4381 gttgaatatt gtttaaatga tttaccggaa gaattattgc cgttagttgc tgaattttta
     4441 ggtgttgact tagatactgc aggtggtttt gccgtaaatg tatgtttgta tacggtgcct
     4501 gatattttta caggtttccc agaatttgtt ggtgaagttg gtaaaacata tactttaaat
     4561 attaattacg aggatcaggt gcttacttct tcaacaacca tgtttccgcc agttccgcct
     4621 gattcaattt attgggaacc acatggcaat cctgaaaatg atactttagt tagattatac
     4681 attgcactct cagaacctga cccacagggt aatttttatc gctattttac acgacaaaac
     4741 agcgaaccat tttattccgg ttattcgagt gtatttgatg ataatttaat taacggtcaa
     4801 tcgtttaaat ttacggtaga cagagggttt aatccttcag aggaatttaa tcaggatacc
     4861 tacggatatt tttggaaagg tgataccatt attttaaaat ggtgcagtat cgattttcct
     4921 acttacgatt tttggcgcac actggaattt gacagtggta cagatggacc gttttcttca
     4981 gcaactattg ctcaaaccaa tattattggc ggacttggaa tttggtgcgc ttatggtgct
     5041 gcttatgata ccttaattgt ggaatagtaa tatccgttat taatagtaga caattttgtg
     5101 aataggggag ccagtaaaca atttaagctt gtatagtaat atatttgtag cttaattgtt
     5161 attatgcaaa aattgttagc tgctttgatt ttgacaattt gtgtgtcaag gggttatgcg
     5221 caaaccagta aaggcgactg gttaacaggt ggtgctgttt atttttctac ttcatccagt
     5281 gaaggcatca cacttaccga cacacttatt acctcctcag tgaattttaa tgcatctgcc
     5341 ttcggtggtt attttgtgat ggataattta tgtgttggac ttgggctcag ttatggaaga
     5401 aacgcagcaa ccaacaatta tgcaccaatt gaacttaccg atgtaagcac ctcactatct
     5461 gttgaagcaa ttacccgata ttattattgg aatggtgacc atgttaaact ttggacaggc
     5521 ttgggcgtgc gaaaaggttt tggtaagtat gatgaccatt tttacgaata cgatgaccaa
     5581 ggcaatggca aagaagcaaa tcaagtagat gatattgcta ctttcagtat tggatttggt
     5641 cctggagctt ccatcatgtt aaatcccaaa ctcgcgctcg aagtatattt tggttcgatt
     5701 ggatattcga cctatacctt agaaagagaa gacggcagtt atgattacac aacaaat
//
LOCUS       DAORKW010000199         5746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_549_length_5746_cov_5.871024, whole genome shotgun sequence.
ACCESSION   DAORKW010000199 DAORKW010000000
VERSION     DAORKW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_549_length_5746_cov_5.871024"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..2674
                     /locus_tag="PLB46_08945"
     CDS             152..2674
                     /locus_tag="PLB46_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76683.1"
                     /translation="MALSPDGKNIAYAIDDHGKFKVYIQEIGSKDADKIKKGGLKTRT
                     LATDLSNPLVSWSPDGKKLAIVYEKRNKNFITTYDTEKKEKESKELTRFQKILDMNFA
                     KTSNRLVLSVMNNGQSDIYVYHLINDRVEQLTNDFFDDLTPCYVELNNGYEGIIFASN
                     RDRDSLYTTRTVDSILPVGNMDLYFYDYIHKSKKLVKISHTKKISETTPLQFDAKHFA
                     YLSDQNGITNRFAGYLDSLYDHTDTLVFYPDSTVTNPTYDYTDLKTWPKFDSMLLADI
                     YRDTAYVFPTTNYAGNIVEHDINWKTGKMVEVFQQGKANVFYLSKAPGDMSEIKNNTL
                     NNTTFRNFINLKKKEEDVITTISNTDSSTMIKTQTIITSTDTIKKEIDEGMYFQTDFP
                     EEITPTIATDPVALGQSKSPEQENMNTGKKFRSSRIVPYRLKFSNEFVTSQLDNSLFI
                     NKYESFNTTGGVYSTPNLGALFTVSISDLFEDYRFTGGMRFPTSFSGGEYYLSYDDLK
                     HRIDKKITWYHQTEKTQYNFQPFWYPVVDAGIRTNVVEGTLKYPFDVIRSLRGSVSYR
                     GEKIIFLANDTFSLNLPNYKEDWLSLKLEYVHDNTFPVMTNILNGMRYKVWFEMHKQF
                     EVNASQGLKISLNDGYMGVFGFDFRHYQKIHRQFTWANRIAGGTSFGPQKLIYYLGGV
                     DSWIAPVFNTDVEINQDAGYAYQTLATNLRGYDQNVRNGNGYIAFNSELRFPIFSYFF
                     NKPLRSEFLRNLQIIGFYDIGSAWEGKSPFEEDNPYTNSTISQTPITVNVNYYRDPLV
                     MGTGVGLRTTLFGYFIRVDRAQPLDASGLGDPRWYFSLSLDF"
     gene            2714..3616
                     /locus_tag="PLB46_08950"
     CDS             2714..3616
                     /locus_tag="PLB46_08950"
                     /inference="COORDINATES: protein motif:HMM:NF017807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRF76684.1"
                     /translation="MKPAFKAHLFILGANIIYGINYAIGKVALEYIEPFGIVLLRVSG
                     ALIIFTLLAYLTGHEKIERGDHKKLLIASLFGVVINQLMFFKGLSLTSEIHSAMIMIA
                     TPIIVLIMAWVILKDKITWIKSTGIIIGGIGVAMLISSGVADKHSPSSLAGDICITIN
                     ATSYAIFLIIAKPLMLKYSPYTIARWIFTYGILFVLPFGWKEVDAISWSSIPAIAIWA
                     LVYVILGATVLAYLFNILGLNYGSPTLVSIYIYTQPVIATIIAVAYGSDHLTVEKIVS
                     AILVFSGVALVSFTGSKKPLSSAV"
     gene            3644..4516
                     /locus_tag="PLB46_08955"
     CDS             3644..4516
                     /locus_tag="PLB46_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRF76685.1"
                     /translation="MIISMTGYGRSTFSLGGKNIVLEMKTLNSKSFDLNLRIAPLFKE
                     YEIEIRNILNKSIMRGKTDCTIGYEMSDDKGGEVNPVAVKEYYRQLKAIANELQIDDS
                     KVFDLVFRLPNVTTTSNESLTEEQWELVQAQIEAACKQLNKFRSDEGINLERDLVLRV
                     QLMREALKEVEALDPQRMEQQRTKMFSDLQNYMGDEDIDKNRFEQELIFYLEKMDITE
                     EVTRLRSHCDYFIETMEETLNEKGKKLGFICQEMNREINTIGSKSYHAEMQRKVVVMK
                     DELEKIKEQLNNVL"
     gene            4519..5088
                     /gene="gmk"
                     /locus_tag="PLB46_08960"
     CDS             4519..5088
                     /gene="gmk"
                     /locus_tag="PLB46_08960"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRF76686.1"
                     /translation="MQGKLVIVTAPSGAGKTTIVKHLLAHENRLSFSVSATTRPIRAN
                     EVDGRDYYFLTEAAFRQKIEEDAFVEWEEVYPGKLYGTLKSEVERIWNSGKHIIFDVD
                     VKGALSLKNKFPERTISIFIEPPSLPILIDRLTKRNTETPATLATRIERVKYELTFAG
                     SFDHIIINDNLELAQSQAFDLVHNFLAGH"
     gene            5123..5503
                     /gene="apaG"
                     /locus_tag="PLB46_08965"
     CDS             5123..5503
                     /gene="apaG"
                     /locus_tag="PLB46_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HRF76687.1"
                     /translation="MVTSITQGVKVSVETFFQPDYSHKGEFVFAYRITIENKSETTVQ
                     LLRRHWHIYDGIGVWREVEGEGVVGETPIIEPGHMHQYVSGCHLVAPMGKMKGTYLML
                     RTTDGNYFDVVVPEFQMIAPFLLN"
     gene            complement(5500..>5746)
                     /locus_tag="PLB46_08970"
     CDS             complement(5500..>5746)
                     /locus_tag="PLB46_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76688.1"
                     /translation="KFFAGEELLFSKRTDDLRRYMDDAPTSVGMPEEAPGRVAAWTGW
                     QIIRAYMRENPDTDLNSLFNEIDAMKILKLSGYGPES"
BASE COUNT         1930 a         1019 c         1083 g         1714 t
ORIGIN      
        1 ttttgtttga ggagcaggtg taaacagcac aatagacgat tggtataaat atttattagc
       61 aaaatatcaa aacgaagcaa aagataaagt aaaacctgat gccgcaaata ttattccgcg
      121 gaaaaccaaa aagaatgtta attatcatga attggcttta agtcctgacg gaaaaaatat
      181 tgcctatgct attgatgacc acgggaaatt taaagtgtat attcaggaaa ttggcagcaa
      241 agatgccgat aaaataaaaa aaggcggatt aaaaacacgc acattagcaa cagaccttag
      301 caacccatta gtgagttgga gccccgatgg gaaaaagtta gccattgtgt atgaaaaaag
      361 aaataaaaat ttcatcacca catacgatac cgagaaaaaa gaaaaagaat caaaagagct
      421 tacgcgcttt cagaaaattc tcgatatgaa cttcgccaaa acaagtaatc gtttggtatt
      481 aagtgtaatg aataatggcc aaagcgatat ttatgtttac catttaatca acgaccgcgt
      541 tgaacaatta acgaacgatt tttttgatga ccttacacca tgttatgttg agctaaacaa
      601 tggatatgaa ggaataattt ttgcatctaa ccgcgaccgc gattcattgt atacaacaag
      661 aactgtggat tctattttac cggttggcaa catggattta tatttttacg attacataca
      721 caaatcaaaa aaactggtga agatttctca cactaaaaaa ataagtgaaa caacaccttt
      781 acagtttgat gcaaaacact ttgcctattt aagcgaccaa aacggaataa caaaccgatt
      841 tgcgggatat ttagatagtt tgtacgacca taccgatacc ttggtatttt atccggactc
      901 aacagtaacc aatccaacct acgactatac cgatttgaaa acatggccaa aatttgattc
      961 catgttattg gctgacattt atcgcgacac tgcatatgtt ttcccgacaa ctaattatgc
     1021 gggcaatatt gttgagcatg atataaattg gaagacaggc aaaatggtag aagtatttca
     1081 acaaggaaaa gccaatgtat tttatttgtc gaaagcaccg ggcgatatga gtgagataaa
     1141 aaacaataca ctcaacaata ctacattccg caattttatc aatttaaaga aaaaagaaga
     1201 agatgtaatt actactatta gtaataccga ttcttctact atgattaaaa cgcaaaccat
     1261 aattaccagt accgatacta taaaaaaaga aattgatgag ggcatgtatt tccaaaccga
     1321 ttttccggaa gaaattacgc caacaattgc cactgaccct gttgcgctcg gacagtcaaa
     1381 aagtcctgaa caggaaaaca tgaataccgg taaaaaattc cgttcttcgc gcatagttcc
     1441 gtatcgcctg aagtttagca acgaatttgt aaccagccag cttgacaata gtttatttat
     1501 caataaatac gaaagtttta ataccacagg cggcgtttat tcaacaccta atttaggcgc
     1561 attatttaca gtaagtatta gcgatttatt tgaagactat cgatttaccg gtggcatgcg
     1621 attcccaaca tctttttccg gtggagaata ttatttatcc tacgacgatt taaaacaccg
     1681 cattgataaa aaaattacct ggtatcatca aacagaaaaa acgcaatata attttcaacc
     1741 attttggtat cctgtagtag atgcaggcat cagaacaaat gttgttgaag gaacactcaa
     1801 atatccattt gatgtaattc gcagtttgcg tggaagtgtt tcgtatcgag gtgaaaaaat
     1861 tatatttcta gctaatgata catttagttt aaacttaccg aattataaag aagactggct
     1921 atcgttaaaa ttagaatatg tgcacgacaa tacttttccg gtaatgacca atattttaaa
     1981 tggtatgcgt tataaagtat ggttcgaaat gcataaacaa tttgaagtaa atgccagtca
     2041 gggattaaaa atcagtttaa atgatggtta catgggtgtg tttggatttg atttccgtca
     2101 ttatcagaaa atacatcgac aatttacctg ggcaaacaga atagccggtg gcacatcttt
     2161 tgggccacaa aaattaatct attatttagg tggtgttgat agttggatag cacctgtttt
     2221 taatacagat gttgaaataa atcaggatgc cggttatgcg tatcaaacgt tagcaaccaa
     2281 tttacgcggc tacgatcaaa atgttcgaaa tggcaatggt tatatcgcat ttaactctga
     2341 attacgtttt cctattttct cttatttctt taataaacca ttgcgttctg aattcttaag
     2401 aaacctacaa attattggat tttatgacat cggttcagca tgggaaggta aatctccttt
     2461 cgaagaagac aatccatata ccaattcaac aattagtcaa accccaatta cagttaatgt
     2521 aaactattat cgcgacccgc ttgtaatggg aaccggtgta ggattgcgca caacattatt
     2581 tggttacttt attcgtgttg accgtgctca accactcgat gcaagtggac ttggcgaccc
     2641 acgttggtat ttctcgctta gtttagattt ctaattacca cacatagttg atgcagttat
     2701 gtaattttgc gccgtgaaac ctgcatttaa agcccatctt tttattctgg gagcaaatat
     2761 tatttatgga ataaattacg cgataggcaa agttgcactc gaatacattg agccatttgg
     2821 cattgtatta cttcgtgtaa gtggtgcttt aatcatattt acacttttgg cttatttaac
     2881 cgggcatgaa aaaattgagc gtggtgacca taaaaaatta ttaattgctt ccttatttgg
     2941 ggtagtgatt aaccaactta tgtttttcaa agggcttagc cttacttctg aaatacattc
     3001 tgcaatgatt atgattgcca caccaattat agtattaata atggcatggg ttattctaaa
     3061 agataaaatc acctggataa agtcaacagg tattataata ggaggtatcg gtgttgctat
     3121 gctaatttca tcgggtgttg ctgataaaca ttccccttca agtttggcag gtgatatttg
     3181 tattactatt aatgccactt cttatgccat ttttttaatc attgccaaac cattaatgtt
     3241 gaagtattca ccgtatacaa ttgccagatg gatttttact tacggaattt tatttgtgtt
     3301 gccttttggc tggaaagaag tagatgccat ttcatggagt agtattcctg ctattgccat
     3361 ttgggcattg gtatatgtaa ttttaggcgc aacagtactg gcttatttat ttaatatttt
     3421 agggctcaat tacggttcac ccacattggt aagtatttat atttacaccc aaccggtaat
     3481 tgcaacaatt attgcagttg catatggcag cgaccaccta accgtagaaa agatagtttc
     3541 cgcaatatta gtttttagtg gagtagcctt agtaagtttc acgggcagta aaaaaccttt
     3601 gagttccgcc gtataattgt atttttacat tcaaattagc agtatgataa tatcaatgac
     3661 aggttatgga agaagcacat tttcgcttgg cgggaaaaat attgtgcttg aaatgaaaac
     3721 attaaatagt aaatcgtttg acctcaacct gcgaattgct cctttattta aagaatacga
     3781 aattgaaatc cgcaatattc tgaataagtc tatcatgcgc ggaaaaaccg attgtacaat
     3841 tggttatgaa atgagtgatg ataagggagg agaagtaaat cctgtcgctg taaaagaata
     3901 ttatcgccag ttaaaagcta ttgccaacga attacaaatt gacgactcaa aagtatttga
     3961 cctggtattc cgtttaccaa atgtgactac tacttcaaat gaaagtctga ctgaagagca
     4021 atgggaatta gtgcaggcgc aaattgaagc cgcatgtaaa caattaaata aattcagaag
     4081 cgatgaaggt ataaatcttg aaagggattt ggttttgcgt gtacaattaa tgcgcgaagc
     4141 gttgaaagaa gttgaagcac tggacccaca gcgtatggaa cagcaacgca caaaaatgtt
     4201 tagcgactta caaaattata tgggtgatga agatatcgat aaaaacagat tcgagcagga
     4261 gttaattttt tatttagaaa aaatggacat caccgaagaa gttacccgcc ttcgctccca
     4321 ttgcgattat tttattgaaa ccatggaaga aacacttaac gaaaaaggta aaaaattggg
     4381 atttatttgt caggaaatga acagggaaat aaataccatt ggttcaaaat cttatcacgc
     4441 cgaaatgcaa cgtaaagttg ttgttatgaa agatgaactc gaaaaaataa aagaacaatt
     4501 aaataacgtg ttgtaaccat gcaaggtaag ctcgtaattg taacagcgcc atcaggtgca
     4561 ggtaaaacaa ctattgtaaa acatttgctt gcccacgaaa accgcttgtc attttctgta
     4621 tctgctacaa cacgccccat tcgcgcaaat gaagttgatg gccgcgatta ttattttttg
     4681 acagaagctg catttagaca aaaaatagaa gaggatgctt ttgtggaatg ggaagaagta
     4741 tatccgggta aattatatgg cacattaaaa agtgaagtag aacgcatttg gaacagcggg
     4801 aagcatatca ttttcgatgt tgatgtaaaa ggtgcattat cgctaaaaaa taaatttcct
     4861 gaacgcacca tttccatttt tattgaacca ccttccttgc caattttaat cgacagactt
     4921 accaaacgca atactgaaac accagcaact ttggctacca ggattgaacg tgttaaatat
     4981 gaactcacct ttgcgggcag tttcgaccat ataattatca acgacaacct ggaactcgcg
     5041 caatcacaag catttgacct tgttcataat tttttagccg gacattaaca gcattccaat
     5101 ttttagctac ttttacatgg gcatggtgac ttctataact caaggtgtaa aagttagcgt
     5161 tgaaacattc tttcaaccgg actactctca caagggtgag tttgtatttg cctatagaat
     5221 aactattgaa aacaaaagtg aaaccaccgt acaattactg cgccgacact ggcatattta
     5281 cgatggaatt ggcgtttggc gtgaggttga gggagaaggt gtagtaggcg aaacccctat
     5341 aattgaaccg ggacatatgc accaatacgt atcgggctgc cacttagtag ctcctatggg
     5401 caaaatgaaa ggcacttatt taatgcttcg cacaactgat ggcaactatt ttgatgtagt
     5461 agtgcctgag tttcaaatga ttgcgccttt tttgttgaat taactctcag ggccgtatcc
     5521 agacagtttc aaaattttca tcgcgtctat ttcattaaac aggctgttca aatcagtatc
     5581 gggattttca cgcatataag cacgtattat ctgccatcca gtccatgcag ccactctgcc
     5641 aggggcttct tcaggcatac ctacagaagt tggcgcatca tccatatacc tacgtaaatc
     5701 gtccgttctt tttgaaaaca gtaactcttc gccggcaaaa aatttc
//
LOCUS       DAORKW010000200         5744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_550_length_5744_cov_5.002109, whole genome shotgun sequence.
ACCESSION   DAORKW010000200 DAORKW010000000
VERSION     DAORKW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_550_length_5744_cov_5.002109"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLB46_08975"
     CDS             <1..368
                     /locus_tag="PLB46_08975"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRF76689.1"
                     /translation="MSVHMHGKVRWMSADELVQLMKTFTLKHEGGDSSSPTIYDNLPE
                     TYVDKMMAGITGFEIKVNKLDNVFKLSQNRDEKSYMNIIAKLEAKGGESALIAEEMEK
                     RKADLFPEGEVWDANKFES"
     gene            516..1097
                     /locus_tag="PLB46_08980"
     CDS             516..1097
                     /locus_tag="PLB46_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF76690.1"
                     /translation="MDKDILSEYIQNLVKMPADKSQLLAEGFENYLLPKNMLLMEENK
                     ISRETYFLESGIIRSYTYDNTGNEVTTNIYTAPCFVNDFLSFFKQQPTSESFQALTDC
                     TIWKMSYEQVQFYFHTYPEFREFGRMMLVTNYSELHNRMLGMIKDTAETRYLTLMQKH
                     PDIFKHVPLKIIASYLGITDTSLSRIRKEIALK"
     gene            1176..1910
                     /locus_tag="PLB46_08985"
     CDS             1176..1910
                     /locus_tag="PLB46_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76691.1"
                     /translation="MEIFTNAVPTWFSALFLMCFLLPIIMIVRAVKTAAINSNINPTS
                     AKKTPQMVALFLGLYYLFVALMSYTGIFQKNTLPPKILGFTALPLIVFYFLVVYRAKI
                     FWTLLKNIKLSALVRIHLFRFVGIFFILGWQFDILPKSFALIGGLGDIFVAFTAIFVA
                     RLIDKKVPNYKRITLIWNIIGLWDILSVLFSAIIITKQAIATNTTGILEMTKFPFSLI
                     PAFAPATIIFLHICIFKKLKMQDKTS"
     gene            2370..2978
                     /locus_tag="PLB46_08990"
     CDS             2370..2978
                     /locus_tag="PLB46_08990"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HRF76692.1"
                     /translation="MLKQFAEESEKNRFETFIDAILAIIMTILVLEFKVPEEAFESDG
                     DIKSFLHHLTPSFLSYLISFSTIVILWMDHHNLFRLLKKVDIPLIFLNFLFILFLSAT
                     PFTTSLAGRNYESAYAVTIVAGNYILMNLTFSSIWAYAIKNKMIKEDAQIQVASKREN
                     GIILAGIGLQVASIPLAYVNTLIPFFLFIVVLILHIYRLSKH"
     gene            3122..3559
                     /locus_tag="PLB46_08995"
     CDS             3122..3559
                     /locus_tag="PLB46_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76693.1"
                     /translation="MALVFFGCQNLTFAQCDLILCDQAPSDDTIPCEIDIDVTQYLEN
                     EVEYDPYTGDSIGVELAKTLYFIVGCVDPFEVDKLTFVLRETMDAAANIYKDIESKTL
                     EVQPDWNFGWISWKFTREGIFWMDVRYNDVTFSTTLVEIDTHE"
     gene            4195..>5744
                     /locus_tag="PLB46_09000"
     CDS             4195..>5744
                     /locus_tag="PLB46_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HRF76694.1"
                     /translation="MHTILPFLLLMVVVIVLLQMWAKHLKIAYPILLVVGGLTISFLP
                     GLPHINVNPDMIFFMFLPALLFEAAWTISFKEMRKWWRIIGSFAFLVVFLTALAVALF
                     TNYLIPGFTLALGFLLGGIISPPDAVSTGAITKFVKIPKSLSAILEGESLLNDASSLI
                     IFRFALIAVGTGQFIWQEASLSFLWMVAGGVGIGLVLAWIFVQAHKRLPTDAPSDIAL
                     TLVEPYLIYWVAEQIHSSGVLAVVAAGLYMSSRNLTFLNSGSRVMGYSVWESFVFILN
                     GVVFLIIGLQLPQIVDGLEASGIPLKTAIGYGVLVTGVIIIVRIISSYAALLATFIFR
                     PAVAPRFTSKKRLLLMPMLLGWTGMRGVVSLAAALAIPVQINGADFPYRNLILFITFV
                     VILLTLVIQGLTLPILIKRSKVFDAISEEPDEEFRKKMKIGLREQSYLFLKEKYENEP
                     ENDVVMQKIIQHWEEKTKAGDQTWMNDKIKSILIEMLEVQRKYLTELNKDPEIDEEII
                     RTQLYQID"
BASE COUNT         1809 a         1035 c         1022 g         1878 t
ORIGIN      
        1 atatgagtgt gcatatgcat ggtaaggttc gttggatgtc ggcagatgaa ttagttcaac
       61 tcatgaaaac gtttacatta aaacatgaag gaggcgattc ctcctctcct actatttatg
      121 ataaccttcc tgaaacttat gtcgataaaa tgatggcagg aataaccgga tttgaaatta
      181 aggtgaataa attggacaat gtatttaaac tgagtcaaaa cagagatgaa aaaagttaca
      241 tgaatattat tgccaaactg gaagcaaaag gtggagaaag tgcattaatt gccgaggaaa
      301 tggaaaaaag aaaagcggat ttatttcctg agggtgaagt atgggacgcg aataaattcg
      361 aatcgtaaga atctaccttg cggcagacag gttcctttta taagttaata aattgcctcc
      421 aaacaataat aaactcctca atccaaaaat atttccggta atgttttaac tgattgcggt
      481 aattttgtaa actcaaaagc aataagcttt ttacaatgga caaagatatt ttatcagaat
      541 atattcaaaa tttagtaaaa atgcccgcag ataaatcaca gttgctggca gaggggtttg
      601 aaaattattt attacctaaa aacatgttgc tgatggaaga aaataaaatc agcagagaaa
      661 cttatttttt ggaatcgggg attattcgct cttatactta tgataataca ggaaatgaag
      721 taactaccaa tatttacaca gctccctgtt ttgtcaacga ttttttatct tttttcaaac
      781 aacaaccaac atctgaatcg tttcaagcat taactgattg cactatttgg aaaatgagtt
      841 atgagcaggt gcaattttat ttccatactt atcctgagtt tagagaattt gggcgtatga
      901 tgttggtaac gaattactcc gaactccata accgtatgtt aggcatgata aaggatactg
      961 cagaaacacg gtatctaaca ttgatgcaaa aacatcctga tattttcaaa catgtcccat
     1021 taaaaataat tgcgtcctac ctgggcatta cagatacatc attgagtaga atcaggaaag
     1081 aaattgccct gaaataaata cttgtcaaat ggcaagaata atatcttcca gcttaaagaa
     1141 ttttgtgaca tcaattcact taatgctttg ttattatgga aatcttcacc aatgctgtcc
     1201 caacctggtt ttcagccctg tttttaatgt gttttctgct tccgattatt atgattgttc
     1261 gagcagttaa aactgcggca attaactcca atatcaaccc aacatcagcc aaaaaaaccc
     1321 cacaaatggt tgccctcttt ttagggttat attatctatt tgtcgcctta atgagttata
     1381 ccggaatatt tcagaagaac acattgccac caaaaatatt agggtttaca gcgttaccac
     1441 tgattgtatt ttatttttta gtagtttata gagcaaaaat attttggaca ttgctaaaaa
     1501 atataaaatt atctgcattg gtgcgtatac acttatttcg gtttgtcggt atatttttta
     1561 ttctcggttg gcagtttgac attttaccaa aatcatttgc cttgattggt ggacttggag
     1621 atatatttgt tgcatttaca gccatttttg tagccaggct aattgataaa aaagtgccta
     1681 attacaaaag aattaccctg atctggaaca tcattggtct ttgggatata cttagcgtat
     1741 tgtttagtgc aattattatc accaaacaag caatagcaac caacaccacc ggtattttag
     1801 aaatgacgaa atttccgttt agtttaatac ctgcatttgc gccggcgacg ataatatttt
     1861 tacatatctg cattttcaaa aaacttaaaa tgcaggataa aacatcctga ttttttcgcc
     1921 ggtcgggaga ccggggtggc gtctggagtt aaaaactcca cgacaacggc gcaacttcgt
     1981 tgcttgaggg gttagtcgta aatttttcat tcttgtaaca gcaaaatgct tatcgctatt
     2041 caaccagtgt tcaaatattt gtcgcaatta ttgaattcac atcaaattgt acatcaaata
     2101 cataaaccaa ctcggtgagg tcgaagaccc tcaccagttg tcgtggagtc tttgactcca
     2161 gacgccaccc cggtctccag accgctgcaa taaacccgca atcgaagccc tccctgctct
     2221 gtaacacctt caacaagccc ccatcctaaa aaaacctacc caaactcaaa aaataatccc
     2281 attttttata atttaagcct gacttttaag taacaaacaa ctgattactt gaagttaccc
     2341 aagtaccatt tacccaaaaa atacccaata tgcttaaaca atttgctgag gaaagcgaaa
     2401 aaaacaggtt tgaaacgttc attgatgcca ttttggcaat tatcatgacc attttggttt
     2461 tagaatttaa agtaccggaa gaggcatttg aatctgatgg tgatattaaa tcatttctcc
     2521 atcatttgac gccttctttt ttgagttatt taatctcttt ttctactatc gttattttat
     2581 ggatggacca tcacaattta tttagattgc tgaaaaaagt tgatattcca ctcatatttt
     2641 taaatttttt attcattttg tttttatcag ctactccatt tactacatca ctggcaggca
     2701 gaaattatga aagtgcttat gccgtaacaa tagttgcggg taattatata ttaatgaacc
     2761 tgacattttc ttctatttgg gcctatgcta taaaaaacaa aatgattaag gaggatgcgc
     2821 aaatacaagt tgcatcgaaa cgagaaaatg gcattatact agccggcatt ggccttcaag
     2881 tggcaagtat tcctttagct tatgtcaata cacttatccc gtttttttta ttcatagtgg
     2941 tactaatttt gcatatttac cgtttaagta aacactaatc aatcattaaa aaacaaaccg
     3001 ggattttgaa aaagtgtaat tctcttttca gccaattttt acctttgtaa aaaattaatt
     3061 tttccaaatg ttatcattta atctgccttt atttaattta ttgaagatga aacatatttt
     3121 catggcactt gtattttttg gatgccaaaa cctcaccttt gcacaatgcg atttgatttt
     3181 atgcgaccag gcaccaagtg acgatactat tccatgtgaa atagatattg atgttactca
     3241 atatttagaa aacgaagtgg aatacgaccc atataccggc gactcaattg gtgttgaact
     3301 cgccaaaacc ttatatttta ttgtaggttg tgttgatcca tttgaagtgg ataagctcac
     3361 ttttgtgctt cgcgaaacta tggatgcagc agcgaacatt tataaagaca tcgaatcaaa
     3421 aacacttgaa gtgcaaccgg attggaattt cggctggatt agctggaaat ttactcgtga
     3481 aggtatattt tggatggatg tgcggtataa tgatgtcact ttttccacaa cactggtgga
     3541 aattgatacg catgaataat tgacttccgg atttagcaaa catcaaataa agtatttggt
     3601 gctatgattt aggtaactaa attgcatttt acattattag gaaattagat tatgcgacaa
     3661 ttatttttaa tatatctgca ttttaaaaaa aaatcaattt tgggaataaa acatcttgat
     3721 tttttcgccg gtcgggagac cggggtggcg tctggagtta aaaactccac gacaacggcg
     3781 caacttcgtt gcttgtgggg ttagtcgaaa atttttcatt cttgtaacat caaaatgttt
     3841 atcggcaatt aaccagtgct gaaaaatttg tcgcaattat agaattcaaa tcaaattgta
     3901 catcaaacac aaaaaccaac tcagtgaggt cgaagaccct caccggttgt cgtggagttt
     3961 tcaactccag acgccacccc ggtctcctga ccgctaccct aaaactacaa gcgaagctta
     4021 accaaattgg tcatgaataa tcccaaatct ataacacctt tagtgcaacc tcgccagaaa
     4081 ttgttaccca caataaatta acccctttaa ccccaattcc aattaaacca tatccttggt
     4141 tattccgaac tttaatttat tcaaatcggt ttttattgat taactttcaa attcatgcat
     4201 accatattac cctttttact gttaatggtt gttgttattg tcctgctgca aatgtgggca
     4261 aaacacctta aaatagccta tccaatttta ttggtagtag gcggattaac cataagtttt
     4321 ttacccggtt taccccatat taatgttaac ccggatatga tattttttat gtttttgccg
     4381 gctttattat ttgaagcagc atggactatt tcatttaaag aaatgcgcaa atggtggcgt
     4441 attatcggga gttttgcatt tttagtggta tttcttactg cattagcagt tgccttattt
     4501 acgaattatc taattccggg atttacttta gctttgggat tcttattagg tggcatcatt
     4561 tccccacccg atgcagtcag tacaggtgct atcaccaaat tcgtgaaaat tccgaaatcg
     4621 ttgtccgcaa tcttggaagg cgagagttta ttaaatgatg cctcttcatt aatcattttc
     4681 cgttttgctt taattgcagt cggcacggga caatttattt ggcaggaagc ctcattaagt
     4741 tttttatgga tggttgccgg tggcgttgga ataggtttag tattagcctg gatttttgtc
     4801 caggcacaca aacgtttacc aactgatgca ccttccgata ttgcattaac tcttgttgaa
     4861 ccctatctta tttattgggt tgcagaacaa atacatagtt caggtgtatt ggctgttgtt
     4921 gcagcaggtt tatatatgtc gtcgcgcaac ttaacatttt taaatagtgg cagtcgggta
     4981 atggggtata gtgtttggga aagttttgta tttattctca atggtgtagt atttcttatt
     5041 atcggtttac aacttcctca aattgtggat ggattagaag cttcaggtat tcccttgaaa
     5101 acagcaattg gttatggtgt tttagtcact ggcgtaatta tcattgtccg tatcatcagc
     5161 tcatatgccg cattattagc tacatttatt tttcgtccgg ctgttgcgcc gcgattcacc
     5221 agtaaaaaaa gattactgtt gatgcctatg ttactgggtt ggacaggcat gcgtggtgtg
     5281 gtgtcacttg cagcagcatt agccataccg gttcaaatta acggggcaga ttttccttac
     5341 cgcaatctca ttttatttat cacatttgtt gttattttat taacccttgt catacaaggc
     5401 cttacattac caatattgat taaacgttca aaagtatttg atgccatttc agaagaaccg
     5461 gatgaagaat tccgtaaaaa aatgaaaatc ggcttgcgcg aacagagtta tttattttta
     5521 aaagaaaaat acgaaaacga accggagaat gatgttgtaa tgcaaaaaat tattcaacat
     5581 tgggaagaaa aaacaaaagc aggcgatcaa acgtggatga atgataaaat taaatctatt
     5641 ttaatcgaaa tgcttgaagt gcaacgcaaa tacctgaccg aactcaacaa agatcctgaa
     5701 atcgacgaag aaataattcg cacccaatta taccaaatcg attt
//
LOCUS       DAORKW010000201         5738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_551_length_5738_cov_5.023931, whole genome shotgun sequence.
ACCESSION   DAORKW010000201 DAORKW010000000
VERSION     DAORKW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_551_length_5738_cov_5.023931"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PLB46_09005"
     CDS             complement(<1..970)
                     /locus_tag="PLB46_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF76695.1"
                     /translation="MALDSKIPAGDLQQKWEDYKNHVKLVNPSNKRKLEIIVVGTGLA
                     GSSAAATLAELGYKVKAFCYQDSPRRAHSIAAQGGINAAKNYKNDGDSVYRLFYDTIK
                     GGDFRAREANVYRLAEVSVNIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARG
                     QTGQQLLIGAYQALERQIGLGNVQMYSRHEMLDVVTIDGKARGIIARDLVTGKLERHF
                     GHAVLLCTGGYGNVFFLSTNAMGCNVTAAWKAYKRGAYFGNPCYTQIHPTCIPVSGEY
                     QSKLTLMSESLRNDGRVWVPKTQEDAKASREGRKKPTDIAEDARDYY"
     gene            complement(1008..1712)
                     /locus_tag="PLB46_09010"
     CDS             complement(1008..1712)
                     /locus_tag="PLB46_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRF76696.1"
                     /translation="MSWMNTFLTSSIGRKLVMSVTGLFLITFLIVHCTINSMIFFNDG
                     GEAFNKAAHFMGTNILIRTMEIVLFAGLILHIVQAYMLAAKNRQARPQGYAVSNPGAN
                     SKWYSRSMTLLGTLILLFLVLHISHFWSNSRLGGMMGIAPLEEVQIGDKEVLNLYQDM
                     IVTFQSPFVIVIYVLGVISLCWHLIHGFQSAFQTLGVNHKKYTPVIKNIGIGFSILIC
                     LLFALMPVSIYAGWIC"
     gene            1938..2126
                     /locus_tag="PLB46_09015"
     CDS             1938..2126
                     /locus_tag="PLB46_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76697.1"
                     /translation="MKKRGLFLLLISLFAACAKEPLFETHSRNAAIIYIDNKPVTITA
                     DSFDYDTVLIINSNPFYK"
     gene            complement(2160..2720)
                     /locus_tag="PLB46_09020"
     CDS             complement(2160..2720)
                     /locus_tag="PLB46_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76698.1"
                     /translation="MKNSIIAILIGALILFVYQALAWMVLPVHANSHKYTATEDSLLA
                     AMNGLEEGVYYLPGTPPNIGKSEAQKLHENNVGKPWAMLYYHENLEMNMGKSMGLGFL
                     IDLIAVALTVRLIQLGNITGKSNILRATMLIAFIIVFSTAMMNWNWFSTPWHFLTGEI
                     LDQLIGGLLLGLWLGFILGRKPKTAN"
     gene            2771..3769
                     /locus_tag="PLB46_09025"
     CDS             2771..3769
                     /locus_tag="PLB46_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76699.1"
                     /translation="METFNTSSIFFPARFWCVALILGLFYNTSRAQALIGGELTYSLD
                     GAIVTFYVTTYSLNAPNSEAWLEYGDGGSSFPELTSVMINDSVYQNYGSASHIFFASG
                     TYLVSCKADKLIGDVRNIEDAGTKILYMDILFYNNIDFINTPPLHTTSIFDFEITDDC
                     YVTHDESASDAEGDSLVYTNSLFEVYGGDDYSSWPESTDSIGFADSGLFYWRNAVYRD
                     LYALQFETKEYRDGILVSETVRKIITPVNCGIVSNTDYVNPTINIFPNPVSDILHVAL
                     PLKTIYTYSIFDVTGRLVARNTNNTTTCDVQHLSPGLYILVIQQNGDKMTSTFNKQ"
     gene            3826..5091
                     /locus_tag="PLB46_09030"
     CDS             3826..5091
                     /locus_tag="PLB46_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76700.1"
                     /translation="MKFLHKLGLTILLLSAMFQARASYILLPMDNGQADHLKCYGIAY
                     WILENGGKLDWMLNYRGGSFCFEYLDVFEKECLIRGVSYEVIPDASYNKIVADIANPE
                     VNMDVMKLEKAPKVAVYSPKSKQPWDDAVTLVLTYSEIPYTLIYDTEVLNGELPKYDW
                     LHLHHEDFTGQYGKFWSAYHTQAWYQQQEEENETIAHEFGYSSVSQLKLGVAHKIRDF
                     VSGGGFLFAMCSAADSYDIALSAEGVDICESMFDGDPADPAAQQKLDFNKTFAFENFT
                     ISKNPYEYSLSNIDVSHSRTVPKELDFFTLFDFSAKWDPVPTMLTQCHEKTIPGFFGQ
                     TTAFNKKLIKSSVLIMGENKSANEARYIHGEYGYGTWSFYGGHDPEDYKHFVGDPPTE
                     LELHPNSPGYRLILNNVLFPAAKKQKQKT"
     gene            complement(5180..5674)
                     /locus_tag="PLB46_09035"
     CDS             complement(5180..5674)
                     /locus_tag="PLB46_09035"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRF76701.1"
                     /translation="MPIKIFVTGGTFDKEYDEIRGQLYFNETHLPEILRLGRCTVNVQ
                     IETLMMIDSLHMSELDRQHIVQHCSDCTNTRIIITHGTDTMVETAKAIAEAKIEKTII
                     LTGAMIPYKFGSSDGLFNMGCALSFVQTLPHGVYITMNGRYFVWNNVRKNKEEGFFEE
                     LANS"
BASE COUNT         1795 a         1125 c         1020 g         1798 t
ORIGIN      
        1 gataataatc gcgggcatct tcagcaatat cggtaggttt ttttctgcct tcgcgactag
       61 cttttgcatc ttcttgtgtt ttaggcaccc aaacacgtcc atcgttacgc aaactttcac
      121 tcatcagcgt cagtttactt tgatactcac cggaaaccgg gatacaggta gggtgaattt
      181 gagtataaca aggattgcca aaatatgctc ctcttttata tgctttccag gctgctgtaa
      241 cgttacaacc catggcattg gtagaaagga aaaatacgtt gccataacca cctgtgcata
      301 ataataccgc atgtccgaaa tggcgttcca atttaccggt cactaaatca cgggcaataa
      361 taccgcgtgc tttgccatca atggtaacca catcaagcat ttcgtggcgg gaatacattt
      421 gtacattgcc caaaccaatt tggcgctcta atgcctgata agcgccgatt aataattgtt
      481 gtccggtttg accacgcgca taaaatgtgc gggaaacctg tgcaccacca aatgaacgat
      541 tatctaataa tccaccatat tcgcgggcaa aaggcacacc ttgcgccaca cattggtcga
      601 taatgtttac actcacttca gccaaacggt aaacatttgc ttcacgcgca cggaagtcgc
      661 ctcccttaat agtatcgtaa aacaaacgat atacggaatc gccgtcgttt ttataatttt
      721 ttgcagcatt aatccccccc tgtgcagcta tcgagtgcgc acgacgcgga gaatcctggt
      781 aacaaaatgc ttttacttta taacctaatt cagccaaagt tgcagccgcc gatgaaccgg
      841 ctaaacctgt tcctacaact atgatttcca atttccgctt attggaggga ttaaccagtt
      901 taacgtggtt tttgtaatct tcccatttct gttgaagatc gcctgcgggt atttttgaat
      961 ctagtgccat tgaataaatt gctgtttaaa ttgatattgg gataaggtta acaaatccag
     1021 cctgcgtaaa tggagacagg cataagcgcg aataaaaggc atatcagtat ggaaaatcca
     1081 attccgatat ttttaatcac cggggtatac tttttatggt ttacacccaa tgtttgaaat
     1141 gccgattgaa atccgtgtat taaatgccag cataatgaaa taacgcctaa tacataaatt
     1201 acaattacaa acggactttg aaaagtaact atcatgtcct gatataaatt gagcacttct
     1261 ttgtcgccaa tttgcacttc ttccaatgga gcaatgccca tcatgccacc taaacggctg
     1321 tttgaccaga aatggctgat atgcaatacc aaaaacaaca aaataagtgt tcctaataaa
     1381 gtcatactgc ggctatacca tttgctgttt gcgcctggat tgctaactgc ataaccctga
     1441 ggccgagcct gacggttttt tgctgccagc atataggctt gcacaatatg caggattaaa
     1501 cccgcaaata gcacaatctc cattgttctg attaaaatgt ttgtccccat aaaatgggct
     1561 gctttgttaa aagcttcacc cccatcgttg aaaaatatca tcgaattgat ggtacaatga
     1621 acaataagga aggtgattag aaacaaaccg gtaaccgaca tcactagctt tcggccaatg
     1681 gaagaggtta aaaacgtatt catccaactc atgtattaaa gattttcaca attgcaaatg
     1741 taaaacagta agattgaatt atatgtttac caacagatag aattatccat aatattttaa
     1801 caaagcttat tttgaatttg tctaaataag gaagttgaag ccatattact atttcaaaat
     1861 ttccgatatt taaattccta ataaaaatag cagctgtaaa ccacgatgtt taatgtcacc
     1921 atattccagt ataaaggatg aaaaaaagag gattattctt attacttatt agtctgtttg
     1981 ctgcctgcgc aaaagagccg ttgttcgaaa cacattcaag aaatgccgct attatttata
     2041 tcgacaataa acctgttaca attactgctg attcatttga ttatgatact gttttaataa
     2101 ttaattcaaa ccctttttat aaataaaaaa agcggcaatt tttattaccg cttttgaaat
     2161 taatttgctg tttttggctt tcgtcctaaa ataaatccca accacaatcc gagtaataaa
     2221 cctccaatta attgatcaag aatttcaccg gttaaaaaat gccaaggtgt actaaaccaa
     2281 ttccaattca tcattgcagt actaaacaca ataataaatg caattaacat agttgctctc
     2341 agaatattac tttttcccgt aatatttccc aattgaatta gccttactgt taatgcaact
     2401 gctatcaggt caatcagaaa tccaagcccc atacttttac ccatattcat ttccagattt
     2461 tcgtgataat acaacattgc ccaaggtttg ccgacattgt tttcatgcag tttttgtgct
     2521 tccgatttgc caatatttgg cggtgttccc ggcaggtaat aaacaccttc ttcaaggcca
     2581 ttcatagccg ctaataatga atcctctgta gctgtgtact tatgagaatt agcatgaaca
     2641 ggtaatacca tccatgcaag tgcctgatac acaaataaaa tgagtgcacc aatcagaatt
     2701 gcgataattg aatttttcat ttgagtagtt tttggtaatt ttagataaat ttagaacaaa
     2761 tatttctacc atggaaactt ttaacacatc ctccatattt tttcctgctc gtttttggtg
     2821 tgtcgcttta attttaggat tattttataa cacctcacgt gcacaagctt taattggtgg
     2881 tgaattaacc tattcgcttg atggtgctat tgtgactttt tatgtaacta cttattcctt
     2941 aaatgctccc aatagcgaag cttggctgga atatggtgat ggcggctcca gtttccctga
     3001 acttacttcc gtaatgataa atgatagtgt ttaccaaaat tatggttctg catcacatat
     3061 tttttttgcc tccggaacat atcttgtgag ctgtaaagct gataagttga ttggtgatgt
     3121 acgcaacatt gaagatgcag ggacaaaaat tttatatatg gatatattat tttacaataa
     3181 tatagatttt atcaatacgc ctcccctgca taccacatcc atttttgatt ttgaaattac
     3241 cgacgattgt tatgtcacac atgatgaaag tgcatctgat gctgaaggag attcattagt
     3301 gtatactaat tctttatttg aggtgtatgg tggggatgat tatagttcat ggccggaaag
     3361 caccgactca attggttttg cagattctgg attattttat tggcgcaatg ctgtttatcg
     3421 tgatttatat gccttacaat ttgaaacaaa agaataccgc gatggaattt tggtaagtga
     3481 aactgttcgt aaaatcatca caccggtaaa ttgtggtatt gtttcgaata ccgattatgt
     3541 caacccaaca atcaatattt ttcctaaccc ggtaagtgat attttacatg tagcattgcc
     3601 attaaaaact atttacacct attcaatttt tgatgttacg ggaaggttgg ttgcgcgaaa
     3661 tacaaataac acaactacat gcgatgtgca gcacctttct cccggtttat atattttggt
     3721 aattcagcaa aatggcgaca aaatgacctc tacctttaac aaacaataaa aacttaacag
     3781 tttttaatca atttcaggtc tggtttttgc gttatatttg ttacgatgaa attcctacat
     3841 aaactcggtc ttaccatttt acttttaagt gccatgtttc aggcacgtgc ttcttatatc
     3901 cttttgccaa tggataatgg tcaggccgac cacctgaaat gctacggaat tgcctattgg
     3961 atactcgaaa acggcggtaa actcgactgg atgttaaact acagaggcgg cagtttttgt
     4021 tttgaatatt tggatgtttt tgagaaggaa tgtttaattc gcggtgtgag ttacgaagta
     4081 attcccgatg cttcttacaa taaaattgtt gctgatatcg ccaatcctga agtaaatatg
     4141 gatgttatga aattggaaaa agccccaaaa gtagcggtgt attcaccaaa atcgaaacaa
     4201 ccatgggacg atgccgttac ccttgtttta acctattccg aaattcctta taccctgatt
     4261 tatgataccg aagtattaaa tggcgaatta cctaaatacg attggctgca tttacatcac
     4321 gaagacttta ccggtcaata cggtaaattt tggagtgcct atcatacaca agcatggtat
     4381 cagcaacagg aagaagaaaa tgaaaccata gcacatgaat ttggttacag cagtgtttca
     4441 caattaaaat taggagtagc acataaaatt cgtgatttcg tcagcggtgg cggattttta
     4501 tttgccatgt gcagcgcagc cgacagctac gatattgcat taagcgccga aggagtagat
     4561 atttgcgaaa gtatgtttga tggagacccg gcagaccctg ctgcacaaca aaaattagat
     4621 tttaataaaa ccttcgcttt cgaaaatttt accattagta aaaatccgta cgaatattca
     4681 ttatcaaata ttgatgtgtc acattcaaga actgtcccaa aagaattgga ttttttcact
     4741 ttatttgatt tttcagcaaa atgggatcct gttccaacta tgttgacaca atgccatgaa
     4801 aaaacaattc ccggattttt tggacagacc acagcgttta ataaaaaatt gataaaaagc
     4861 agcgtattaa ttatgggtga aaataaatcc gctaacgaag cgcgatatat ccatggcgaa
     4921 tacggttacg gcacatggtc attctacggc ggtcacgacc ctgaagatta taaacacttt
     4981 gtaggtgacc ctcctactga attagagtta catccaaatt ctccggggta ccgactaata
     5041 ttaaataacg tgttatttcc tgctgcaaaa aaacaaaaac aaaaaaccta aatcgggttg
     5101 taaaattcgt acgaattaat ggtaattctg gtgcacaaaa atgaaggtgt gccacaagta
     5161 ttttacagat caacaggaat caactattgg caagctcctc gaaaaaacct tcttctttat
     5221 ttttgcggac attattccaa acaaaataac gaccattcat ggtaatataa acaccatgtg
     5281 gtaatgtttg cacaaaagat aacgcgcaac ccatattaaa taacccgtcg gagctgccaa
     5341 atttatacgg aatcatagct cctgttaaaa tgatggtttt ttcaattttt gcctcagcaa
     5401 ttgctttggc tgtttcaacc atagtgtcgg ttccatgtgt aataatgatg cgtgtgttgg
     5461 tacaatcgct gcaatgttga acaatatgct gacgatctaa ttcggacata tgcagactgt
     5521 caatcatcat cagggtttca atttgtacgt ttacagtaca ccttcccaat cgcaaaattt
     5581 ctggtaaatg tgtttcatta aaatacagtt ggcctctaat ttcatcatat tccttatcaa
     5641 aggttccacc tgtaacaaat atctttattg gcatttcggg tgattattcg ttctttaaaa
     5701 aattggctgc tcctcttatg aaacttatca gaataagg
//
LOCUS       DAORKW010000202         5717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_554_length_5717_cov_5.596079, whole genome shotgun sequence.
ACCESSION   DAORKW010000202 DAORKW010000000
VERSION     DAORKW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_554_length_5717_cov_5.596079"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /gene="rfbD"
                     /locus_tag="PLB46_09040"
     CDS             complement(<1..730)
                     /gene="rfbD"
                     /locus_tag="PLB46_09040"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRF76702.1"
                     /translation="MKILVTGSKGQLGNEIQVLSKRHPQHNYVFVDVDELDITNNTSV
                     QQFFAQHQFDVCINCAAYTAVDKAESDNTMAALINVTGVEYLANACQRAGAVLFHIST
                     DFVFDGSATSPIDVATSVNPISVYGQTKAEGEYRALSLNQKTILIRTSWVYSTFGNNF
                     VKTMIRLGKEKPELGVVNDQVGCPTYAKDLAWVIFEFVEKLPNIEYGIYHFSNSGVTS
                     WCGFAQKIMELYGLNCKVNPITTNQ"
     gene            complement(742..1299)
                     /gene="rfbC"
                     /locus_tag="PLB46_09045"
     CDS             complement(742..1299)
                     /gene="rfbC"
                     /locus_tag="PLB46_09045"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRF76703.1"
                     /translation="MPFIDTPIPGLFVFEPKVFEDARGYFFESFNAAVFSEKGINVNF
                     VQDNQSKSTYGILRGLHYQLDPFAQAKLVRVISGEVLDVAVDVRKGSPTFGQHFGIIL
                     SAENKKQLYIPRGFAHGFVVLSETAEFFYKCDNFYSKEHDAGILYNDNTLNIDWKINP
                     DDIKVSEKDANLPTFPNALNNFQYK"
     gene            complement(1307..2044)
                     /locus_tag="PLB46_09050"
     CDS             complement(1307..2044)
                     /locus_tag="PLB46_09050"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76704.1"
                     /translation="MLLSLFGKLFSKKDIVDYSVIKTDMHSHLIPGIDDGAKDEQTTL
                     LMIEGLKALGFTQLITTPHIMKSYFDNNEQTILSGQQNFNAKYQTDIIAAAEYFVDEE
                     FIQKIKSNTPLLTFGDNYVLIEVSMGVKDKQLEEAVFELTSRNYKPVLAHCERYPYMF
                     EHGKLNYYEKLKDADVLLQVNIRSFLGQYGEIQKKIARKLAINDMIDFLGTDIHNEMQ
                     LPMLKDALHDEYVQHLLNSNQLKNALL"
     gene            complement(2047..3102)
                     /gene="rfbB"
                     /locus_tag="PLB46_09055"
     CDS             complement(2047..3102)
                     /gene="rfbB"
                     /locus_tag="PLB46_09055"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRF76705.1"
                     /translation="MKKNLLITGGAGFIGSHVVRLFVNKYPDYHIVNLDKLTYAGNLN
                     NLTDIEKASNYEFVKADIVDGDAMVQLFQKYQFEKVIHLAAESHVDRSIANPIEFVIT
                     NVVGTCNLLNAARHIWKDNSEGKLFYHVSTDEVYGSLGEEGFFTESTPYDPHSPYSAS
                     KASSDHFVRAYHDTYKLPVVISNCSNNYGSFQFPEKLIPLAINNIKNNRPIPVYGKGE
                     NVRDWLWVDDHARAIDVILHKGKVGETYNIGGFNEWKNIDLIHVLCKVMDEKLGRTPG
                     TSASLITFVTDRAGHDLRYAIDATKLNTELGWQPSLQFEEGIAKTVDWYLANEAWLNN
                     VTSGDYKKYYDNQYAAR"
     gene            complement(3105..4394)
                     /locus_tag="PLB46_09060"
     CDS             complement(3105..4394)
                     /locus_tag="PLB46_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRF76706.1"
                     /translation="MYQQLSNKNTKIAVIGLGYVGLPIALEFGRIFSVIGFDIKKERV
                     NSLNKKIDPDGEVPASAFDGTDVLFTNNVDDLNGCSFHIIAVPTPVDEHKVPDLSYLE
                     SATTIIAKILKKGDYVVYESTVFPGCTEEICLPILEKQSGLLLNQDFKIGYSPERINP
                     GDTQYTLRNVIKIVSGSDEEALENIAQIYSAIVQPGVYRASSIKVAEAAKILENTQRD
                     LNIALMNELSLILDKMQVNTYEVIEAAGTKWNFHKYYPGLVGGHCIGVDPYYLTYKSM
                     ELGYEPYVILSGRKVNDNMAFYIARRCMQQLIAAGKNLTDSKILVMGLTFKENVNDIR
                     NSKVADLIHELKSFGLHVDVTDPKADKAETEKEYGFSLTTVNGTKYDAIILSVPHKEY
                     IGLDERYFLSIAKEQCLFIDIKGVFRKKITQLNYWSL"
     gene            complement(4415..5548)
                     /locus_tag="PLB46_09065"
     CDS             complement(4415..5548)
                     /locus_tag="PLB46_09065"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRF76707.1"
                     /translation="MRKIQMVDLSTQYQQIKQEVDAGIQEVIDTTQFINGTATKTFAT
                     ALGNYLNVKHVIPCANGTDALQIALMALDLKEGDEIITIPFTFVATVEVVALLKLVPV
                     FVDVDPDTFNIDVNAIEAKITPRTKAIIPVHLFGQSADMEKIMAIAAKHNIVVIEDNA
                     QAIGCDYTFSDGTTKKTGTIGHIATTSFYPTKNLGAYGDGGALFTNDDELAKKVRIIC
                     DHGSAKKYYYDSIGVNSRLDSMQAAILNAKLKRLDTYNEKRQQAAALYDAQLANIPGI
                     QIPARAKNATHVFHQYTIRVAENRDALKDHLAALGIPAMIYYPVPLHMSDAYASYGYK
                     TGDFPVSEKIAHEVLSLPMHTELDEEQIQFICQSIKSFITVPA"
BASE COUNT         1743 a         1117 c          944 g         1913 t
ORIGIN      
        1 attggttggt tgtgatggga ttaaccttgc agttaagtcc gtataattcc ataatttttt
       61 gtgcaaatcc acaccaggat gtaacaccgg aattagaaaa atggtaaatg ccatattcga
      121 tgtttggtaa tttttctaca aattcaaaaa tcacccaagc aaggtcttta gcataagtgg
      181 gacagccaac ctggtcattc acaacaccaa gttcaggttt ttcttttccc aaacggatca
      241 ttgtttttac aaaattattt ccgaaggtgc tgtaaaccca actggttctt attaatatcg
      301 ttttttgatt gagtgataat gcccggtatt caccttcagc ttttgtttgc ccgtaaacag
      361 aaataggatt cactgaagtt gccacatcaa ttggtgaggt tgctgagccg tcaaaaacaa
      421 aatcggtaga aatatgaaat aagactgctc ccgctcgttg gcaggcattt gccaaatatt
      481 caacgccggt tacattaatg agcgccgcca tggtattatc actttctgct ttgtcaacag
      541 cagtgtatgc tgcacaattg atacaaacat caaattgatg ttgtgcgaaa aattgctgaa
      601 cactcgtatt attagtaata tccagttcat ccacatctac aaaaacataa ttatgctgag
      661 gatggcgttt gcttaacacc tgaatttcgt tacctaactg acctttagaa cctgttacca
      721 gaattttcat tgtgttgtgt tttatttata ttggaaatta tttaatgcat ttggaaaagt
      781 aggcaaattt gcatcctttt cactcacttt aatatcgtca ggattaattt tccagtctat
      841 attcagcgtg ttatcattgt aaagtattcc ggcatcatgt tctttgctat aaaaattgtc
      901 gcacttataa aaaaactctg ccgtttcact taatactaca aaaccatgcg caaaaccgcg
      961 tggtatataa agttgttttt tattttctgc tgacaaaata ataccaaagt gttgcccaaa
     1021 agttggtgaa cccttgcgga catcaaccgc aacatctaac acttctccac ttattacacg
     1081 caccagttta gcttgtgcaa atgggtcgag ttggtaatgt aatccacgca aaataccata
     1141 agtagattta ctttggttat cctgcacaaa atttacgtta attccttttt cactaaaaac
     1201 ggcagcatta aaagattcga aaaaataacc tcgtgcatct tcaaatactt ttggttcaaa
     1261 aacaaataaa cccggaattg gcgtgtcaat aaatggcata tagttgttat aataaagcgt
     1321 tttttaattg attactattt agtaaatgct gcacatattc atcatgtaaa gcatctttaa
     1381 gcattggcaa ctgcatttca ttatgaatat cagttcctaa aaaatctatc atatcgttaa
     1441 tcgccaattt tctggcaatt tttttctgga tttctccata ttggcctaaa aaagaacgga
     1501 tgtttacttg taataacaca tcagcatctt taagcttttc ataataattt aattttccat
     1561 gttcaaacat atatggatat ctttcacaat gtgccaacac cggtttataa tttcgggatg
     1621 taagttcaaa aacagcttct tctaattgtt tatctttaac ccccatactc acttctatca
     1681 gcacataatt atcgccaaat gtcaatagcg gtgtattact ttttattttt tgaataaatt
     1741 cctcgtctac aaaatattcg gctgcggcaa taatatctgt ttggtattta gcattaaaat
     1801 tttgttgccc gcttagaatt gtttgttcat tgttatcaaa ataagatttc attatatgag
     1861 gagtcgtaat caactgggta aatcccaatg cttttaagcc ctcaatcatt aataaagtag
     1921 tttgttcatc tttggcacca tcatcgattc ccggaatgag atgcgaatgc atatctgttt
     1981 ttattaccga ataatcaacg atatcttttt tagaaaataa cttgccaaat aatgagagca
     2041 acatgattac cttgctgcgt attgattatc gtaatatttt ttataatcgc cactggttac
     2101 attgttgagc catgcttcat ttgccaaata ccaatctacc gttttagcaa taccttcctc
     2161 aaactgcagt gatggctgcc aacctaattc agtatttagt ttagtcgcat caatcgcata
     2221 acgcaaatcg tggcctgcac gatcggttac aaaagtgatg aggctggctg aagtgcccgg
     2281 tgtgcgacct aatttttcat ccattacttt gcacaataca tgaatgaggt caatattttt
     2341 ccattcatta aatccaccaa tattataggt ttctcctact ttgcctttat gtaaaatgac
     2401 atcaatagct ctggcatgat catccaccca taaccaatcg cgaacatttt cgccttttcc
     2461 gtaaaccgga atcgggcgat tattttttat gttatttatc gccagtggaa ttaatttctc
     2521 cggaaactgg aatgaaccat aattattact gcaattagaa ataacaaccg gtaatttata
     2581 ggtatcgtga taagcacgaa caaaatggtc gctgcttgcc ttacttgccg aatatggact
     2641 atgtgggtcg taaggtgttg actcagtgaa aaatccttct tcccctaaac tcccatacac
     2701 ctcgtctgtt gaaacatgat aaaataattt gccttcgcta ttgtctttcc aaatatggcg
     2761 tgcggcattt aataagttac aagtaccaac cacattggtg ataacaaatt ctattggatt
     2821 ggcaatactt ctgtccacat gactttccgc agctaaatga ataacttttt caaattgata
     2881 tttttgaaag agttgcacca tagcatcacc atcaacgata tccgctttta caaattcata
     2941 atttgaagct ttttcgatat ctgtcaggtt attcagatta ccggcatagg taagtttatc
     3001 caaattcaca atatggtaat ccggatattt attgacaaat aaacgtacaa catgtgatcc
     3061 gataaatccg gctcctccgg taataagcaa attctttttc atagttataa actccagtag
     3121 tttagttgag tgattttttt tctaaaaacg cctttaatat caataaacaa acattgttct
     3181 tttgctatcg acaagaagta acgttcatct aagccaatat attctttatg cggtacagat
     3241 aaaatgattg catcgtattt tgttccattc acggtggtaa gtgaaaaacc gtattctttt
     3301 tctgtttctg ccttatctgc tttagggtct gtaacatcca catgtaatcc aaacgactta
     3361 agttcatgga ttaagtctgc aaccttagaa tttcgaatat cgttaacatt ttctttaaat
     3421 gttaaaccca ttaccaatat tttcgaatca gtaagatttt tacctgcggc aattaattgt
     3481 tgcatacatc tgcgtgcaat ataaaaggcc atattatcat ttacctttct gccacttaaa
     3541 ataacataag gttcatatcc aagttccatg gatttatagg taagataata cgggtcgaca
     3601 ccgatacaat gtccaccaac taaaccggga taatatttat gaaaattcca ttttgttccg
     3661 gctgcttcaa ttacttcgta agtatttact tgcattttat ccagaattaa tgaaagctca
     3721 ttcatgagcg caatattcaa atcgcgttgg gtgttttcca aaattttagc tgcttccgct
     3781 actttaatgg atgatgcgcg gtatacacct ggttgcacaa tggcactata aatctgcgca
     3841 atattctcaa gcgcttcctc atctgaaccg gaaacaattt taatcacatt gcgcaaggta
     3901 tactgtgtat caccaggatt gatgcgttca ggtgaatagc caattttgaa atcctgattt
     3961 agcagtaaac ctgattgttt ttcgagaata ggcaaacaaa tttcttctgt gcaacccgga
     4021 aatacagttg attcatacac cacataatca ccctttttta atattttggc aataattgtt
     4081 gtggcagatt ctaaataaga taaatccggt actttatgtt catcaacggg cgtaggaaca
     4141 gcaattatgt gaaaactaca tccgtttaaa tcatcaacat tattggtaaa taacacatcg
     4201 gtaccatcaa aagcagatgc aggaacttca ccatcagggt caattttttt atttaaacta
     4261 tttacccttt cctttttaat atcaaatcct ataactgaaa aaattctacc aaattccaat
     4321 gcaattggca ggcctacata tcccaaacct attacagcaa ttttagtatt tttattactc
     4381 agttgttgat acatgaatat aataattgtc gcaattatgc gggtacagta ataaatgatt
     4441 taatggactg acaaataaac tggatttgtt cttcgtctaa ttcagtatgc ataggcaatg
     4501 ataatacctc gtgagcaatt ttttcactaa ccgggaaatc gcctgtttta taaccataac
     4561 ttgcatacgc atcactcata tgtaaaggaa ccggataata aatcattgcc ggtataccta
     4621 aagcagcaag atggtctttt aaggcatctc tgttttcggc aacacgaata gtatattgat
     4681 gaaaaacatg ggttgcattt tttgcacgtg caggaatttg gatgcccgga atattggcaa
     4741 gttgtgcatc ataaagtgca gcagcttgtt gacgtttttc attataggta tcgaggcgtt
     4801 ttaattttgc atttaaaatt gccgcctgca tactatccaa acgagaattt acgccaatag
     4861 aatcgtaata atatttttta gcagagccgt ggtcacaaat tatgcgtact ttttttgcca
     4921 gttcatcatc attggtaaac aaagcaccgc cgtcaccata tgcacccaaa tttttagtcg
     4981 ggtaaaaaga ggttgttgca atatgaccga tagttcctgt tttttttgtt gtgccatctg
     5041 aaaaagtata atcacaacca atagcctgag cattatcttc aattactaca atattgtgtt
     5101 ttgcggcaat tgccataatt ttttccatgt cggcactttg tccaaacaga tgtacgggaa
     5161 ttatagcttt tgttcgaggt gtaatcttag cctcaatcgc gttaacatca atattaaatg
     5221 tatcagggtc aacgtcgaca aaaaccggta ccaatttgag taatgcaaca acttcaaccg
     5281 tagccacaaa tgtgaaaggg atagtaataa tctcatctcc ttcttttaaa tcaagcgcca
     5341 taagggcaat ttgtaaggca tcggtaccat ttgcacatgg tattacatgt tttacattga
     5401 ggtaattgcc taatgctgtg gcaaaggttt tcgtagccgt cccattgatg aattgagtgg
     5461 tatcaataac ttcctggatt ccggcatcca cttcttgttt aatttgttga tactgagtac
     5521 tcaggtcaac catctgtatt tttctcattc ccgtgtcaaa tttcagggtg caaagatact
     5581 taaaccatag tgaaggcgtt tacaaacccg gcaatctttg taaatttgcc cgctttctta
     5641 tgaaattcaa ccacctgatt ttgctttttt gcctgtttgg cagtcgcctt agcgctcaaa
     5701 taaacctgaa cgacagt
//
LOCUS       DAORKW010000203         5705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_557_length_5705_cov_4.806549, whole genome shotgun sequence.
ACCESSION   DAORKW010000203 DAORKW010000000
VERSION     DAORKW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_557_length_5705_cov_4.806549"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="PLB46_09070"
     CDS             complement(<1..1357)
                     /locus_tag="PLB46_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76708.1"
                     /translation="MAHNNHLKTAPSTNKITFLICVFTFLTFASIHAQSVNIEWQQIY
                     GGTNADGAYDIDATADGGFITGGYSYSGISGNKSVTNYGDSDYWVVKTDAAGNIEWQK
                     TYGGSAFEQVETIAQTADGGYIVGGYSVSGISGNKTEANVAGSIDLWIVKLDASGNIT
                     WQNAIGGNGSDYLGSIYPTSDGGYIVGAYSYSGISGDKTEATVGFSADFWILKLNASG
                     NIVWQNTIGGTSSDYCFRAKQAADGNYIVSGVSSSPISGDKTENSTGGMDVWVLKLNT
                     SGGIIWQNTINASETETAYEMDITADGGAIIGTQTYSNSSPDKSENNISGGITTNDYW
                     IIKLDASGNVEWENTIGGSAYDYNHGLLVMSSGEIVVAGYSLSPISGDKTQPLVGLAD
                     YWVIKFDSAGNYIYDKTIGGTVADYCYGLTETLDGKFVLCGSSASNISGDKTTNTYGL
                     ND"
     gene            1610..2848
                     /locus_tag="PLB46_09075"
     CDS             1610..2848
                     /locus_tag="PLB46_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76709.1"
                     /translation="MNLKEYSLLAAGILAASDANNQTVYQDINPDLSLTHDDQVWFNI
                     STPLDLNNDGVTDFDFKLWGYSTYSGWAAQGYFIFGYAVPKAGHQVLIASSQSPGWCT
                     FGWFAENFDHIANYSNGEIINPTPATGAFVHPENEFYNNQLLFMQREKDYFASTWYFF
                     CTGVPDPVEQGGLWFDAEMRVMPIKFKQSGNYYTGWVRLSVSEGNLTVHDYAINLTPE
                     TPIVAGDIAGAIIAQAPVPGLVTTLSTKAYLTWSPVPDVYKYRYRYRKVGTLIWTEKY
                     HTGTTKLIKSLDCASNYEWQVQTVYDNSPEMNSVWSPLHTFSTMSCRVGGEDVIVEDV
                     KIYPTLASTQIIVQTTSQSQTVDAIVEMFNLQGAQVKSFVTNDKTFEMDVSDLPTGTY
                     VVVVHIGDDEPVIEKVMIGR"
     gene            complement(2910..5069)
                     /locus_tag="PLB46_09080"
     CDS             complement(2910..5069)
                     /locus_tag="PLB46_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76710.1"
                     /translation="MKTLKSHLGCLALLSIFSIQLSHTQIAYQWQKAYGGSLNDGAYS
                     ISTTNDGGTIVGGYSYSGISGIKTESAFGLGDYWVLKLDADGNVEWQKTYGGSGLDVL
                     VQVVQTADGGYFVGGYSQSPISGNKTENSLAGTPDIWLLRIDAVGNILWQTDLGGNGT
                     DYLGDVNETSDGGCIIGGYSNSSNSGDKAENSKGNLDYWIIKLNSDGSIGWQNTIGGT
                     SIDQCHAAEQLPDGTYFISGYSSSPISFDKTEAPVGPFNNYDAWIMQLNASGSIIWQN
                     VIGGSSVENLYAAKITPDNGAILAIQSSSNISGDKTENTISGVAGDMDYWLVKVDHLG
                     NIEWQNTLGGIHADLCYDVVLTTSGGYAVAGHSLSGVSGDKTNGNWGDTYDYWAIGVD
                     SLGAVIWDKTYGGLEEDYGYAMSGNENGQFVIAGNSASGISGDRTAASYGGFDFWLIK
                     FRPVCDSTGEVCNTLDDDCDGLIDESVIEDIYIYNLGETSVCQGAGVTMFAEYTGASV
                     QWTRNGIPLPGATGPGYVALASGNYACITSSDCGEATSNIIHVTINKKPKAKVVAGGP
                     TTFCDGDFVYLNELPTAGCTYQWQKDGTSIPGAVLNTYVATTTGNYRCIVTKTETGCF
                     NTSNSILVNVTCKTEVSHINQVNIFPNPANDAVNVYTSLSGVKQIKLMNAFGQLIQQT
                     TTNEELVMFDVTNLPAGFYIIEINYNNSIIQQTCIIE"
     gene            complement(5182..>5705)
                     /locus_tag="PLB46_09085"
     CDS             complement(5182..>5705)
                     /locus_tag="PLB46_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76711.1"
                     /translation="KNPKAIISAAGPTTFCVGGNVTLNVSPVAGCSYQWYKDALPIPG
                     ATATNYLATTAGIYKCRVTKIATGCFKTSAGITVSVPCKEGLPDDAAGEVTTDKQILV
                     YPNPTDEIVFIELPENTNALITIYNNLGQQVQQQTCYNEMAEIVVEHFPAGIYFIELQ
                     HYNTNQSLSFCKQ"
BASE COUNT         1745 a         1169 c          980 g         1811 t
ORIGIN      
        1 aatcgtttaa tccataagta tttgttgttt tatcgccgga gatattagat gccgaagagc
       61 cacacaaaac aaactttcca tctaaggttt cagtaagtcc gtaacaataa tccgcaacag
      121 tacctcctat tgttttatca taaatatagt tcccggctga atcaaattta atcacccaat
      181 aatctgccaa tcctacaagc ggttgagttt tatctccgct gattggagaa agcgaatatc
      241 ctgcaacaac aatttctcct gagctcatca ctaataagcc gtgattataa tcataagccg
      301 atccaccaat agtattttcc cattcaacat ttccacttgc atctaattta attatccaat
      361 aatcattagt agtaattcca ccggaaatat tgttttcaga tttatccgga gaactattgg
      421 aataagtttg cgtaccgata attgcgccac catctgctgt gatatccatt tcataagcag
      481 tttcagtttc tgaagcattg attgtatttt gccaaataat acccccggag gtattgagtt
      541 ttagcaccca aacgtccatt ccacctgttg agttttctgt tttatcgcct gaaattggtg
      601 aactagaaac cccggaaact atataatttc catcagccgc ttgtttagca cgaaaacaat
      661 aatcgctgga tgttccacca atggtatttt gccaaacaat atttccgctt gcatttaatt
      721 ttaaaatcca aaaatctgct gaaaaaccaa cagttgcctc agttttatca cctgaaattc
      781 cactgtatga ataagcccca acaatataac caccatcaga agtcgggtat atggacccta
      841 aataatcgct gccatttcct cctatggcat tttgccaggt aatattgcca ctggcatcaa
      901 gtttaacaat ccataaatca attgaacctg ccacatttgc ttccgttttg ttacctgaaa
      961 taccggaaac tgaatatccg ccaacaatat atccgccatc agcagtttgt gcaattgttt
     1021 caacctgttc gaatgctgag ccaccgtatg ttttttgcca ttcaatgttg ccggcagcat
     1081 ccgtttttac cacccaataa tcggaatcac cataattggt taccgattta tttcctgata
     1141 tacctgagta gctataacct cctgtaataa agcccccatc ggctgttgcg tcaatatcgt
     1201 atgcgccatc cgcattagtt ccaccataaa tttgttgcca ttcaatgttt accgattgcg
     1261 catgaattga cgcgaatgtt aaaaaggtga atacgcatat taaaaaggta attttgttcg
     1321 tcgacggtgc agtttttaaa tgattgttgt gtgccataag aacttttatt tttaaaattg
     1381 tagctaaaat tacaaattct tatggaagtg gccagaattt aacaatgttt aggttaggtg
     1441 acgaaaatta tctgataagt gtgcttaatt tagcaataaa tgtagggcta cattaacccc
     1501 tttgctgcag caatttgaac aaatattact ttagttaaaa aagtaaaatt agattacctt
     1561 ttttgcgtta ttttttgtaa tattgaaaca caaattaaaa ctcaccctta tgaatctaaa
     1621 agaatacagc ttgcttgctg cgggaattct tgcagcttca gacgccaaca atcagactgt
     1681 ttatcaggac attaatccgg atttatcgct tacacatgat gaccaggtat ggtttaatat
     1741 ttctacgccc ttggatttaa ataatgacgg ggttactgat tttgatttca aattatgggg
     1801 ttatagcacc tattccggat gggctgctca aggatatttt atttttggat atgcagtgcc
     1861 aaaagccggt catcaggtat tgattgcgtc gagccaatcg cccggctggt gcacctttgg
     1921 ctggtttgcc gaaaacttcg accatattgc caattatagc aatggtgaaa ttattaatcc
     1981 gacaccggca acgggtgcgt ttgtacatcc tgaaaatgaa ttttataata atcagctgtt
     2041 atttatgcag cgcgaaaagg attatttcgc ctccacatgg tatttctttt gcacaggtgt
     2101 tcctgatcct gttgaacagg gcggattatg gtttgatgct gagatgcgtg ttatgccaat
     2161 taaatttaaa caatcaggca attattacac cggttgggtg cgattgagtg tgagtgaagg
     2221 gaatttaacg gtacacgatt atgctattaa tttaacgcct gaaaccccta ttgttgccgg
     2281 tgatattgca ggtgcaatta ttgcgcaggc tcctgttccg ggattggtta ccacactttc
     2341 aaccaaagct tatttaacat ggtcgccggt acctgatgta tataaatatc gttaccgtta
     2401 tcgcaaggtt ggaacactca tctggacaga aaaatatcat accggtacta ccaaactaat
     2461 taaatcgttg gattgtgcaa gtaattatga atggcaggtt caaaccgtat atgataattc
     2521 gccggaaatg aattctgtat ggagtccttt gcatactttt tcaaccatga gttgcagagt
     2581 tggtggcgaa gatgttatag ttgaagatgt aaaaatttat ccgacacttg catcaactca
     2641 aataattgtt caaactacca gtcagtcgca aacagttgac gccatagtag aaatgtttaa
     2701 cctgcaaggc gctcaggtaa agagtttcgt gacaaacgac aaaacgtttg aaatggatgt
     2761 aagcgattta cctacaggca cttatgtggt ggtagtgcat attggagatg atgaaccggt
     2821 gattgaaaaa gtgatgatag gtagataatt caaactaata aaaaacactt attgtaaata
     2881 ttacaataag tgttttttta tatacttaat tattcaatta tacatgtttg ttgaataatc
     2941 gaattattgt aattaatttc gattatataa aaacctgccg gcaaatttgt tacatcaaac
     3001 attaccaatt cttcatttgt tgttgtttgt tggattaatt gtccaaatgc attcatcaat
     3061 tttatttgtt taacacctga aagtgaagta tacacattta cagcatcatt tgctggatta
     3121 ggaaatatgt ttacttggtt aatatgcgat acttcagttt tgcaagttac attaaccaaa
     3181 atactattcg aagtattaaa acaacccgtt tctgttttag tgacaataca gcgataatta
     3241 cctgtagttg tagcaacata agtattcaaa acagcaccgg gaattgaggt gccgtccttt
     3301 tgccattgat aggtgcaacc ggctgtcggc aattcattta aatacacaaa atcgccatca
     3361 caaaatgtag ttggtccacc tgcaacaact ttagctttag gttttttatt tatcgtaacg
     3421 tgaataatat ttgatgtagc ttcaccacaa tcactgctag taatacatgc ataatttcca
     3481 gaagctaaag caacatatcc cggaccggtt gcaccgggta atggtatacc atttcgcgtc
     3541 cattgaacac ttgcacctgt gtattcagca aacatggtga caccggcgcc ttggcaaaca
     3601 gatgtttcac caagattata aatataaata tcttcgatta ccgactcatc aattaatcca
     3661 tcgcaatcat catcgagtgt attacaaact tcacctgttg aatcacaaac cggtcggaat
     3721 ttaattaacc aaaaatcgaa accaccataa gaagcagctg ttctgtcgcc cgaaattcct
     3781 gacgccgaat ttcctgcaat aacaaactga ccgttttcat tgccagacat cgcataacca
     3841 taatcctctt ctaaaccacc gtatgtttta tcccaaatta cagcgcctaa actatcaaca
     3901 ccaattgccc aataatcgta ggtatcgccc caattgccat ttgttttatc gcctgataca
     3961 cctgataatg aatgaccggc cactgcatat cctccgcttg tagtgagcac aacgtcataa
     4021 cataaatccg catgaatacc accaagtgta ttttgccatt caatattacc aagatggtca
     4081 acttttacta accaataatc catatcaccg gctacacctg aaatagtatt ttccgtttta
     4141 tcgcctgaaa tattagagct agattgaata gccaaaatag caccattgtc cggtgtaatt
     4201 ttagctgcat acagattttc cacagaactt ccaccaatta cattttgcca tataatactt
     4261 cccgaagcat tcagttgcat aatccatgca tcataattat tgaatggacc aacaggtgcc
     4321 tctgttttat caaaactaat tggagatgat gaataaccac tgataaaata tgtaccatca
     4381 ggtaattgtt cagcggcatg acattgatca atgctggttc cgccaatggt attttgccag
     4441 ccaattgaac cgtcgctatt taatttaata atccaataat ctaaattgcc tttactattt
     4501 tctgctttat cgccggaatt acttgaatta gaataaccgc ctattataca tccaccatcg
     4561 ctggtttcat ttacatctcc taaataatcg gttccattac cgcctaaatc tgtctgccat
     4621 aaaatgttgc caactgcatc tatacgtaac aaccatatat caggtgtacc cgctaaacta
     4681 ttttcggttt tattgcccga aataggcgat tgagaatatc ctccgacaaa ataaccgccg
     4741 tctgcagttt gcacaacttg aactaataca tcgaggcctg aaccaccata agttttttgc
     4801 cattccacat tgccatcagc atctaatttt agcacccaat aatcaccaag accgaaggct
     4861 gattccgttt taattccaga tataccggag taactataac caccaacaat tgttcctcca
     4921 tcgtttgtgg tactaattga ataggctcca tcgttaagtg agcctccata agctttttgc
     4981 cattgatatg ctatttgagt gtgggataat tgtattgaaa aaatcgataa caatgccaaa
     5041 cagcctaaat gggatttaag tgttttcata tttaaattgt gtttgcacaa atttagccct
     5101 tttttgcagg aatttaagtg tggagaaata aacacattca aattcacggt ttttggtgaa
     5161 tgagaaaagt tttgcagtta attattgttt acaaaatgat aatgattgat tagtattata
     5221 gtgttgtaat tcgatgaaat atatgccggc aggaaaatgc tcaacgacaa tttcggccat
     5281 ttcattataa catgtttgct gttgcacctg ttgtcctaaa ttattataaa ttgtaattaa
     5341 ggcgtttgta ttttccggca actcaataaa tacaatttca tctgttgggt tagggtatac
     5401 caatatttgt ttgtctgttg ttacttcccc tgccgcatcg tcaggtaaac cttctttaca
     5461 tggcacactt accgtaattc cagcagaagt tttaaaacaa ccagttgcta ttttcgtcac
     5521 acgacattta taaataccag ccgttgttgc caaataatta gtcgcagttg cacccggaat
     5581 tggcaatgca tctttatacc attgataact gcaacctgca accggactca catttaaagt
     5641 aacatttccg ccaacacaaa acgtagttgg accggctgcg gaaattattg cttttggatt
     5701 tttaa
//
LOCUS       DAORKW010000204         5688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_560_length_5688_cov_3.981182, whole genome shotgun sequence.
ACCESSION   DAORKW010000204 DAORKW010000000
VERSION     DAORKW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_560_length_5688_cov_3.981182"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2874)
                     /locus_tag="PLB46_09090"
     CDS             complement(<1..2874)
                     /locus_tag="PLB46_09090"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF76712.1"
                     /translation="MKKLLLLLVVVPQLVTAQLVINEIAPNNRVVADEDGQFPDWIEI
                     MNIGAADINVQQYAITDNPDTWNKWLLPNYIIEAGERLLIFASEKNRDCYGCPGIVNY
                     LHTNFKLSNGETVALYDESGNLIDSVTIGNVYATDVMARIPDGGDWCYADTETAKDPN
                     SVTCYTEYSEEPVFNTLPGFYAGSIDVTISGTDIFYSTNGDWPDVDGIAYTAPVTIST
                     TKVVKAVSKQPGKLPSRTITGTFFIDETTQLPVVSISSNSCDMFNEGFSCEAAYDGAD
                     GWEPDNPQIPAGVEYFTADHVQKISKNIKFEAAGNSSIAVYPQKGMQFTADEDFGDNS
                     EYDFNVFENYKPLDSLNGFRVRANNDWGNSNARMKDVINNRLAAPTHIVTPAYQNVAT
                     FINGEYWGLYSAREELDKYFLRNNQGVNPDKVDIIRSGAGEDVWDIAEAGTITAYNEL
                     KTFFNTHSMTNPDHYALALELIDIENWVDHFALQFFVNNDEMAYNLRCFRSYEPDMKW
                     KFILWDTGAGSECPSCNSIETLINFPYLSEEINMMNELVDNTDFRNYFINRYADLMNY
                     YFTWEIVENLIDVNADEIEAEIPAQYGRWGAPSVGAWNSGVNELKDFFEPRVGFQRNE
                     IENYFDLNDQVTITLEVNPPGAGYIKISTIVPENLPWSGVYFDGVPVTVTAIPNPGFT
                     FSNWSDNPFITDATALSFNNNFTDNTTFTANFTGVADFGIVISEVNYNSNTFVNSGDW
                     IELYNPTAVDIVLTAYQIANKEFYTNYKIADNVVLPAGGYLVVAENLAQFSAVYPDVT
                     NVVGDMVFNFSNDGDSIVLRNPVGQLITGFAFEDKFPWPATADGFGRTMEFDFTINEP
                     ASPTSWFAGCVLGSPGMAYSNCFENPIIDEINYHASDAANSGDWFELFNWSAADFDLS
                     GYTIQDESGNSFIIPEGTSVTGDGGYLVFYQDETLFSSQFPDIT"
     gene            3134..4465
                     /locus_tag="PLB46_09095"
     CDS             3134..4465
                     /locus_tag="PLB46_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76713.1"
                     /translation="MKTLTRFLLTFLCLLGSKQLLKAQTDCDNVTSGNVPITEIGPGF
                     FMGYQGGLYPGGSNNMPVAHLTAGKSMANSVKPLNEDGVVDFENGKIVFVSFGASTAG
                     NTFNTFKATVNATPDLYNPCIKYVNLCMGGKGLESMIPPHNNYYWAYIADSLMQDAEI
                     SVNQVQVGWMKTASKDDSIPEFPMQADSLYAKYIPSIKRIKENFPNMKILYLSSHAYG
                     GYAGELSDNADLAGEPAAYYGGFAVKWVIEDQIEGSPELDYTGVDAVSPWLAWGPYFW
                     ADGLTPRVADGLIWECEDYEPDGGGFHLSESGKTKESDILIDFLYNHPSSKKWFRNGP
                     KWSSCDPAGRLADGSVIENDSEADYFEDALTVYPSPNDGNFYVSWNNTVEGNCLMQLV
                     SSQGQVIYNKTLQLAEGDVNQHITIPNIPSGIYFINVMANNTRLTKTLIIK"
     gene            complement(4530..4922)
                     /locus_tag="PLB46_09100"
     CDS             complement(4530..4922)
                     /locus_tag="PLB46_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76714.1"
                     /translation="MTFKTVLIRGLVAAIASSLACIVWNKIYYFAMMTDFSAIINYGS
                     IIGANIFAGVLMAIGYFVITKYVKKNGVLVFNILLATLSIASFYGPLSTSLPLDIMAP
                     ELFLGLAAPMHLFPALAWLTLQPLFEKK"
     gene            complement(4928..5461)
                     /locus_tag="PLB46_09105"
     CDS             complement(4928..5461)
                     /locus_tag="PLB46_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRF76715.1"
                     /translation="MKKIIFPLAAVVLLLASAFTFKSAQTWKIADGYSVAFSSADPSG
                     VFNDLKGSIVFDENDLGASKFDVTIDVNSINTGNGMQNKHAVSKKWFDAETYPTIKFT
                     STSVFKGSTGLMTKGILDMHGVQKEITIPFTIEKTSAGATFKGNFDVNRNDWKIGEPG
                     GTASEVIKLSVSVPVTK"
BASE COUNT         1843 a         1057 c          991 g         1797 t
ORIGIN      
        1 ggtaatgtca ggaaactgac tactaaataa tgtttcatcc tgataaaata ccaaatagcc
       61 tccatccccg gttaccgatg taccttcagg aataataaaa ctattgccac tttcatcttg
      121 tatggtgtaa cctgacaaat cgaaatctgc tgccgaccaa ttaaataatt caaaccaatc
      181 gccggagttt gcggcatctg aagcatgata gttaatttca tctataatcg gattttcaaa
      241 acaattgcta taagccatac ccggagaacc tagcacacat cctgcaaacc aggaagtagg
      301 tgaagcaggt tcattaatag taaaatcaaa ttccattgtc cgcccaaaac catcagcagt
      361 tgcaggccat gggaatttat cttcaaatgc aaatccggta attaactgcc ctacgggatt
      421 acgtaaaaca atcgaatcac catcattgga aaaattaaat accatgtcac caaccacatt
      481 agttacatca ggatatacag cgctaaactg tgctaaattt tcagcaacaa caagataacc
      541 accggcaggt aatacgacat tatcggcaat tttatagttg gtgtaaaatt ctttattcgc
      601 aatttgatat gctgttaaaa caatatccac tgcagtcgga ttatataatt caatccaatc
      661 gccggagttt acaaaagtat tactgttata attaacttca cttataacaa taccaaaatc
      721 ggcaactccg gtgaaattgg ctgtaaatgt cgtattgtcc gtaaaattat tattaaatga
      781 tagtgctgtg gcatctgtaa taaaaggatt gtcgctccag ttagaaaagg taaatccggg
      841 atttggtata gcagtcacgg taaccggcac accatcaaaa tatactccgg accaaggtaa
      901 attttcagga acaatagttg aaattttaat gtagccggca ccgggaggat ttacttctaa
      961 cgtaatggta acctgatcat ttaaatcaaa ataattttca atctcgttgc gctgaaatcc
     1021 aacacgtggt tcaaaaaaat cttttaattc attcacacct gagttccaag cacccacact
     1081 aggtgctccc catcgaccat attgtgcagg aatctccgct tcaatttcat cagcatttac
     1141 atcgattaaa ttttctacaa tttcccatgt gaaataataa ttcattaaat cggcatacct
     1201 gtttataaaa taatttctga aatcagtatt atcaaccaat tcattcatca tattaatttc
     1261 ttcactgaga tacgggaaat taatcagtgt ctctatgcta ttacaagaag gacattccga
     1321 accagctcct gtatcccata aaataaattt ccatttcata tcaggttcat acgagcggaa
     1381 acatcttaaa ttataggcca tttcatcgtt gtttacaaaa aactgtaaag caaaatggtc
     1441 cacccaattt tcaatatcaa ttaattccaa tgctaatgca taatggtcag gattagtcat
     1501 ggaatgggtg ttaaaaaagg tttttaattc gttataagca gttattgttc ccgcctctgc
     1561 aatgtcccat acatcttctc ctgcgccact tctgataata tcaactttat caggatttac
     1621 accttgattg tttcttaaaa agtatttatc taattcttct cttgcggaat aaagtcccca
     1681 atattcacca ttaataaatg tggctacgtt ttgataagca ggtgtaacaa tatgtgtagg
     1741 agccgctaat ctattattaa ttacatcctt catccttgcg tttgaattgc cccaatcgtt
     1801 atttgcacgt actcgaaatc catttaatga gtctaaaggt ttataatttt caaaaacatt
     1861 aaaatcatat tcgctattat caccaaaatc ttcatcggct gtaaattgca tgcctttttg
     1921 cggataaact gcaatggaag aatttcctgc agcttcaaac ttgatatttt tacttatttt
     1981 ttgaacgtga tctgcagtaa aatattcaac acccgccggt atttgcggat tgtccggttc
     2041 ccagccgtct gcaccatcat atgctgcttc acaactaaat ccttcattaa acatatcaca
     2101 ggaattagaa ctgatactaa caaccggtaa ttgtgttgtt tcatctataa aaaatgttcc
     2161 cgtaattgtt ctactaggta atttgccggg ttgtttagat acagctttta ctactttagt
     2221 agtactaatt gtaactggtg ctgtgtaagc aataccatca acatccggcc aatcgccatt
     2281 ggtggaataa aatatgtcag taccacttat agtaacatct atactccctg cataaaaacc
     2341 aggtaaagta ttaaatacag gttcttcaga atattcagta taacaggtta ctgaattagg
     2401 atcttttgca gtttcggtat ctgcataaca ccaatcgcca ccatcgggga tgcgtgccat
     2461 tacatctgta gcatatacat taccaatagt tacgctgtca attaaattac cactttcatc
     2521 atatagtgca accgtttctc cattacttaa tttaaagttg gtgtgcaaat aattgactat
     2581 tccggggcat ccgtagcagt cgcgattttt ttctgaggcg aaaatcaata atcgttcacc
     2641 ggcttcgata atataattcg gcaataacca cttattccag gtatcaggat tatcagtgat
     2701 ggcatattgc tgcacattaa tatcagcagc accaatattc ataatttcaa tccaatccgg
     2761 aaactgacca tcttcatcgg ccacaacgcg attgtttggt gctatttcat taattaccag
     2821 ttgagctgta accaattgcg gaacaactac taatagtagg aggagctttt tcatgtagct
     2881 aatttaacta tttattcata attacttcat tttggatgta attggcgctt tgtttacctg
     2941 caattgccaa gaatagggca aggaagttta aaatttgaat gtattgacat gaaattcggc
     3001 tgaaaactat atggttctag cgtgattgag attaaaaata tcgagccgaa atggagtttt
     3061 tacaaaaaaa agttatcttg tgacctcaaa tttatcaggt atacacacaa attttaaatt
     3121 tttacccttt tttatgaaga ccttaaccag atttttattg acttttttat gccttttagg
     3181 aagcaagcag ctcctcaaag cacaaactga ttgtgataat gtgacatcag gaaatgtacc
     3241 tattaccgaa attggaccgg gattttttat gggctatcag ggcgggttat atccaggtgg
     3301 tagtaataat atgccagtag cccatttaac agcaggtaaa agtatggcaa attctgttaa
     3361 acctctgaat gaagatggcg ttgtagattt tgaaaatggt aaaattgtat ttgtttcttt
     3421 tggtgcatct acagccggca atacgtttaa tacatttaaa gcaacagtaa acgccactcc
     3481 tgatttatat aatccatgta ttaaatatgt aaatctatgt atgggtggca aaggtttaga
     3541 atcgatgatt ccaccgcata ataattatta ttgggcttat attgctgatt cattaatgca
     3601 ggatgcagaa atatcagtta atcaggttca ggttggttgg atgaaaacgg cttcgaaaga
     3661 tgattccatt cctgaatttc ctatgcaggc tgattcatta tatgcaaaat atataccttc
     3721 cattaaacgt ataaaagaga atttccctaa catgaaaatt ctttacttaa gcagtcatgc
     3781 atatggtggt tatgccggcg aattatctga taatgccgat ttagccggag agccagcagc
     3841 atattatggt ggatttgccg ttaagtgggt aattgaagat caaattgaag gctcacctga
     3901 gcttgattat acgggtgttg atgcggtatc accctggttg gcgtgggggc cttacttttg
     3961 ggcagatgga ttaactccaa gagttgctga tggattaatt tgggaatgcg aagattatga
     4021 acctgatggg ggaggatttc atttatctga atctggtaaa actaaagaat cagatattct
     4081 gattgatttt ttatataatc acccaagctc aaaaaaatgg ttccgcaacg ggcctaaatg
     4141 gagcagttgt gatccggcag gaagattagc agatgggtct gtaattgaaa acgattccga
     4201 agcagattat tttgaagatg cacttacagt ttatccttca ccaaacgatg gtaattttta
     4261 tgtttcatgg aataatacgg tggaaggtaa ttgtttaatg caattggtaa gtagccaggg
     4321 acaggttatt tataataaaa cattacagct tgcagaaggt gatgttaatc aacatatcac
     4381 tataccgaat attccttcag gtatttattt tatcaatgta atggcaaata atacaaggct
     4441 gacaaaaaca cttataataa agtgaaaata atggaataaa aaaaaagacc tcaattgagg
     4501 tcttttttta tgttaaataa attttactat tatttttttt caaataaagg ctgcagtgtt
     4561 aaccacgcta atgccggaaa taaatgcatt ggtgcggcta aacctaaaaa taattccggt
     4621 gccattatat ctaacggtaa ggaagtggaa agaggaccat aaaagctggc aatagataag
     4681 gttgctaata ggatattaaa taccaacaca ccatttttct tgacatattt ggtaattaca
     4741 aaataaccaa ttgccattaa tacgcctgca aaaatgtttg cgccaataat agaaccataa
     4801 ttgatgatgg ctgaaaaatc cgtcatcatg gcgaaataat aaattttatt ccaaacaata
     4861 caagccagtg atgaagcaat tgctgctaca agccctctga ttaaaactgt tttaaaagtc
     4921 atacccatta tttagttaca ggtacactaa ctgataattt gataacttcc gatgctgtac
     4981 ctcccggttc accaattttc cagtcgttgc ggtttacatc aaagttgcct ttaaatgtag
     5041 cacctgcact tgttttttct attgtaaatg gaatggtgat ttctttttga acaccatgca
     5101 tatcaagtat ccctttggtc attaaacctg tgctaccttt gaacacagaa gtagaagtga
     5161 atttaatagt aggataagtt tcagcatcaa accatttttt actcactgcg tgtttatttt
     5221 gcatgccgtt tccggtatta attgaattaa catctattgt tacatcaaat ttagatgcac
     5281 ctaaatcatt ttcatcaaaa acgatactgc cttttaaatc gttgaatact cctgatgggt
     5341 cggcactgct aaatgccact gaatagccat ctgcaatttt ccaagtttgt gccgatttaa
     5401 aagtgaaggc cgaggcgagc aacaatacaa ctgctgctaa aggaaagatt atttttttca
     5461 tagtttattc atttttaacc ctaaatgtat gtatatacat ttaagttccc aaaaataggt
     5521 taaatttaat taaatatgtt cgggagggat tggaatgccc taaaaacggg ctattcagga
     5581 aatcgataac cgttgagaaa catttggctg acgttaaaaa tcggatattc accaggtcca
     5641 attataagca taacagccag gttgttgcca tctacccagc ccttaatc
//
LOCUS       DAORKW010000205         5667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_563_length_5667_cov_5.962224, whole genome shotgun sequence.
ACCESSION   DAORKW010000205 DAORKW010000000
VERSION     DAORKW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_563_length_5667_cov_5.962224"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLB46_09110"
     CDS             <1..711
                     /locus_tag="PLB46_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRF76716.1"
                     /translation="PKHIAIIMDGNGRWAKSNGKARIYGHHAGVEAVRKVVETCTELG
                     VQYLTLYAFSTENWKRPEAEVNALMELLVRTIRKETPELDKNNVRIGMIGDGHSLPKA
                     CIDELEEAKKMTSANTGLNLILALSYSGRWEITHAVQQIAQKVQNGELKPADITEKTI
                     SAYLNTSAIPDPELLIRTSGEYRLSNFLLWKSAYTEYYFTETLWPDFDKNELVKALLD
                     YQKRERRFGKTTEQIQVR"
     gene            708..3389
                     /locus_tag="PLB46_09115"
     CDS             708..3389
                     /locus_tag="PLB46_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HRF76717.1"
                     /translation="MSNLVRLLFLCGWMTFFTTLANGQNFGLDSNIVDYGNPKKYTIA
                     ATEIRGVKSPNIDLSSLIVRTGLTPGLPLTVPGEDFSLAIKSLWKMRLFSSVNIVVDK
                     ILGDQIWIGIEVEELPKISAFAFTGTSKSEDEDMRPLLDIVGNVTPYADFTRVHIENT
                     VTDYFAEKGFQNAEIDVYAKTDTSRYNSVIVFIDVIKNAKVKIDKINIEGNQEVSDRK
                     LLKQMKDTRMRTQFNVFYNNPEDPITKADFSPKGIVNILSSLSIDNISDFVAERAQVR
                     IFNSSKYDPDKAKVDAQNIVTYYKSLGYRDAEIKLDSVYDISSRAIGINYTVVEGDKY
                     YFRDIYWKGNTKYSTEKLDSILDIKKGSVYNQTYLNQRISFDLAKPDITSYYMDDGYL
                     FFRITPVEIWAEDDSIDLEIRVFEGPQAIIDQILIRGNTKTSEHVIRRELRTYPGQKF
                     NRSLVIRSQREIIALGLFDAEQIAVNPIPHPESGTVDIEYTVVEKPSDQLELSAGYGG
                     QGYGILASVGIVLNNWSTKQMFEKGAWRPYPSGDGQKVSFRINTTGRLYQAFNIGFVE
                     PWFLGKKPNSFSMSLTRTQLGYNVSGTTYDEIEGNFVANGASVGYGIRLKWPDDYFTL
                     LSSVNYYNYQLTDYPYFIATNGSYNNFSIKETLARNSLDDQQFPKSGANIALSLQFTP
                     PYSLMNNKDYTDLDSEAKFKFIEYYKWRFNADYYAPVGGKFVLRTSLKFGWLGYYNSE
                     VGIPPFERFQLGGDGLSGGYTNTFVGTDVISLRGYEVLNTQGTSSARTEAIFNKYTLE
                     LRYPIVKSQTANIYALAFAEGGNLYPDIESFNPLDLKRSAGLGVRAWLPMFGLLGVDY
                     GIRFDDQIPGDLQEANGFFDYITKNGKFTVILGFEPE"
     gene            3408..3950
                     /locus_tag="PLB46_09120"
     CDS             3408..3950
                     /locus_tag="PLB46_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRF76718.1"
                     /translation="MKKSLLIAIVMIATTITANAQRFAYVNTDYILSNITEYKTAQEQ
                     IDKVTAEWKADVEKKQKEIDELYRNFQNEQYLLTEEQKKAKIGEIETKEKAMKEFQKS
                     KFGYEGELFKKRQELVKPIQDKVYEAIEKYAKERGYDFIFDKSSSTTILFANPENDKS
                     DEIIKRLGYTPQKNSPTEDK"
     gene            3978..4475
                     /locus_tag="PLB46_09125"
     CDS             3978..4475
                     /locus_tag="PLB46_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRF76719.1"
                     /translation="MKIKILFISAFICLTAISANAQKIGYLNTNELLVQMKEVKSADS
                     LLNQYAEEMQKIYASYVMEYQSKLADYQNNAATWSEVKRESVETDLGNLQIRISDFEK
                     ESNQKLETKKQELYNPILEDIKAKIKQVGEENKFTTILDGSAMLYVGSDAVDIMPLMK
                     KKLGI"
     gene            4549..5373
                     /gene="murI"
                     /locus_tag="PLB46_09130"
     CDS             4549..5373
                     /gene="murI"
                     /locus_tag="PLB46_09130"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRF76720.1"
                     /translation="MQKQFPIGVFDSGYGGLTILKEFVARMPEYDFIYLGDNARAPYG
                     NRSFETVYEYTLQCVQKLFDMGCRLVILACNTASAKALRTIQQHDLPAIDSQRRVLGV
                     IRPTSEVVGQLTATNQVGIFATSGTVNSGSYEIEIEKFFPHISVFQEACPMWVPMVEY
                     NEFDKPGADYFIQQHINRLLNKSDEIDTIILGCTHYPLLLPKIKQYLPPHIKVIAQGE
                     IVTDSLIDYLQRHPEMDNMCSKSKSLSFFTTDSTAIFDEHAAIFFGQEVHSEHIHI"
BASE COUNT         1910 a          978 c         1124 g         1655 t
ORIGIN      
        1 cctaaacaca ttgccataat tatggatggt aatggccgtt gggctaaaag taatggcaag
       61 gcacgtattt atggtcatca tgcgggagtg gaagcggttc gaaaagtggt ggaaacttgc
      121 acagagcttg gggttcaata ccttaccctc tacgcattca gcaccgaaaa ctggaaacgt
      181 cccgaagctg aagtaaatgc tttgatggaa cttttggtgc gaactatcag aaaagaaacc
      241 cctgagttgg acaagaacaa tgtgcgaatt ggtatgatag gcgatgggca ttctctgcca
      301 aaagcctgca ttgatgagct cgaagaggca aaaaaaatga cttcggcaaa tacaggatta
      361 aacttaattc ttgcgctgag ctactccggc cgctgggaaa ttacccatgc cgttcaacaa
      421 attgcacaaa aagtgcaaaa cggcgaacta aaacctgctg atatcaccga aaaaacaatt
      481 agcgcttatt taaataccag cgcaatccca gacccggaat tattaattag aaccagcggc
      541 gaatatcgac tcagtaactt tttactgtgg aaatccgcct atacagaata ttattttaca
      601 gaaaccctat ggcctgattt cgataaaaac gagttagtaa aagctttgct cgattatcaa
      661 aagcgcgaac gcaggtttgg taaaacaaca gaacaaattc aagtaagatg agcaacttag
      721 tgagattatt atttttgtgc ggatggatga cgttttttac aaccttggca aatggccaaa
      781 atttcggact agactctaat attgtcgatt atggcaatcc gaaaaaatat accattgcag
      841 ctactgaaat aagaggtgtt aaatcaccaa atatcgacct cagtagtttg attgttagaa
      901 caggtttaac accaggctta ccacttactg ttcccggcga agattttagc cttgctatta
      961 aaagtttatg gaaaatgcgc cttttttctt ccgtaaatat tgtagtagat aaaattttgg
     1021 gcgaccagat ttggattgga attgaagtag aagaattacc gaaaatttct gcatttgcat
     1081 ttacaggaac atcgaaatcg gaagatgaag atatgcgccc gctactcgac attgttggta
     1141 atgtaactcc gtatgctgat tttacaagag tgcatattga aaataccgtt actgattatt
     1201 ttgcagaaaa aggttttcaa aatgctgaaa ttgatgtata cgccaaaaca gatacttcgc
     1261 gttacaatag cgtaattgta tttattgatg ttataaaaaa tgcgaaagtt aaaattgata
     1321 aaatcaatat tgaaggtaat caggaagtaa gcgaccgcaa gttgttaaaa caaatgaaag
     1381 atacgcgcat gcgtacacaa tttaatgtgt tttataacaa cccggaagac ccaattacta
     1441 aagcggactt ttcaccaaag ggaattgtaa atattttatc atcactgagt attgataata
     1501 tttcggactt cgttgcagaa cgtgcacagg tgcgtatatt taattcttca aaatacgacc
     1561 ctgataaagc aaaggtagat gcacaaaata ttgttactta ttataaatca ttaggttacc
     1621 gcgatgctga aattaaatta gattctgttt acgatatcag cagccgcgca attggcatta
     1681 attatactgt tgtggaagga gataaatatt atttccgtga tatttactgg aaaggaaata
     1741 ctaagtattc aacagaaaag cttgattcga ttctcgacat taaaaaaggt tcggtgtata
     1801 atcaaaccta tttaaatcaa cgtatttctt tcgatttagc taaacccgat attacttcct
     1861 actacatgga tgacggttat ttattcttta gaataacacc ggttgaaatt tgggcagaag
     1921 acgatagtat cgatttagaa attcgggttt tcgaaggacc acaggcaatt atcgaccaga
     1981 tattaattcg cggaaatacc aaaacaagtg agcacgttat cagaagagaa ttgcgtacct
     2041 atccgggaca aaaatttaat cgttcgttgg taattcgttc acagcgagaa attattgcat
     2101 taggtttatt tgatgcggaa caaatagcgg ttaatccgat tccacatcct gaatctggaa
     2161 cagtagatat tgagtatacg gttgtagaaa aaccttctga ccaattagaa ttatctgccg
     2221 gttatggtgg ccagggttat ggtattttag ctagcgttgg tattgtgtta aataactgga
     2281 gtacaaaaca aatgttcgaa aaaggcgctt ggcgtccata tccttccggc gatggtcaaa
     2341 aagtgtcgtt ccgtattaat accacaggac gactatatca ggcatttaat atcggatttg
     2401 ttgagccatg gttcctcggt aaaaagccaa actctttttc gatgtcgctt acccgcaccc
     2461 aattaggtta taatgtgagc ggaactactt acgacgaaat tgaaggaaac tttgtagcta
     2521 acggagcatc tgttggttat ggtattcgtt taaaatggcc tgatgattat tttactttat
     2581 tgagttcagt taactattat aattatcagc ttactgatta tccatatttt atcgcaacca
     2641 atggtagtta taataacttc agtataaaag aaaccctggc gagaaattca ctcgacgatc
     2701 agcaattccc taaatcaggt gcaaatattg cgttgtcact acaatttact ccgccgtatt
     2761 ctttaatgaa taataaagat tacaccgatt tagattcaga agcaaaattc aaattcatcg
     2821 aatattataa atggcgtttt aatgcagatt attatgcacc ggttggtggc aaatttgtat
     2881 tgcgcacttc attaaaattc ggatggttag gatattataa ttcagaagta ggtattccgc
     2941 cttttgaaag attccaatta ggcggcgatg gattatcagg tggttatacc aatacattcg
     3001 taggtactga tgtaatttca ttgagaggtt atgaagtgtt gaatacacaa ggtacttcaa
     3061 gtgcaagaac agaagccata tttaataaat ataccctcga attgcgttac ccgattgtga
     3121 aatcacaaac agccaatatc tatgcactgg catttgccga aggtggtaac ctgtatcctg
     3181 atattgaatc atttaatccg cttgacctga aacgcagtgc cggcttgggt gttagggctt
     3241 ggctaccgat gtttggtttg cttggtgtag attatggcat acgctttgac gatcaaatac
     3301 cgggagacct tcaggaagcc aacggatttt tcgattatat taccaaaaat ggaaaattca
     3361 cggttattct tggattcgaa cctgaataat tattaaaaca gcgtaacatg aaaaaaagct
     3421 tattaattgc aatcgttatg attgctacca caattacggc aaatgctcag cgttttgcct
     3481 atgtaaatac cgattatatt ttaagcaata taacggaata taaaactgca caggagcaaa
     3541 tagacaaggt aactgccgaa tggaaagccg atgtggagaa aaaacaaaaa gaaatagatg
     3601 aactgtatcg caatttccaa aacgaacaat atttgctgac tgaagaacag aaaaaagcaa
     3661 aaattggaga aatagaaact aaggaaaaag ccatgaaaga atttcaaaag tcgaaattcg
     3721 gctacgaagg cgaattattc aaaaaacgtc aggaattagt taaaccaatt caggacaaag
     3781 tgtatgaagc catcgaaaaa tatgcaaaag aacgtggtta tgattttatt ttcgacaagt
     3841 cgagcagcac tacaattttg tttgccaacc ccgaaaatga taaaagtgat gaaatcatta
     3901 agcgtttagg atatacgcct caaaaaaatt caccaactga agacaaataa taaaagacca
     3961 aatcagcaaa tcagattatg aaaattaaaa ttttatttat cagcgcattt atttgtttaa
     4021 cagcaattag cgcaaatgca caaaaaatcg gttacctcaa taccaacgaa ttgttggtgc
     4081 aaatgaaaga agtgaaatca gccgattcat tattaaatca gtatgcagaa gaaatgcaaa
     4141 aaatttatgc ttcttatgtt atggaatacc agtcgaaact agccgactac caaaataatg
     4201 ctgctacatg gagtgaagta aaacgtgaat cggtagaaac tgatttgggt aacttgcaaa
     4261 tccgcatcag cgattttgaa aaggaatcta accagaaact ggaaactaaa aaacaggaat
     4321 tgtacaaccc gattcttgag gacatcaagg caaaaattaa gcaggtaggt gaagaaaata
     4381 aatttactac catcctcgat ggctcagcta tgttatacgt gggttctgac gcagttgata
     4441 ttatgccttt gatgaaaaag aaattaggta tctgatagtt tatcattacg ttattttaat
     4501 ttaaaggcgc ctttcggggc gcttttttta tacctttgtt ttgctgttat gcagaaacaa
     4561 tttccaatag gcgttttcga ttcgggctat ggtggcttaa ccatactgaa agaatttgtt
     4621 gcccgcatgc ccgaatatga ttttatttat ttaggcgaca atgcccgcgc accttatggc
     4681 aacaggtcgt tcgaaacggt ttatgaatac acactccaat gcgttcaaaa attgtttgat
     4741 atgggctgcc gcctggttat tttggcttgt aatacagcat cagcaaaagc cttgcgcact
     4801 attcagcaac acgatttgcc tgctatagat tcgcagcgtc gggtattggg tgtaattcgt
     4861 ccaacatcgg aagtagttgg ccaattgact gctactaatc aggtgggaat atttgctacc
     4921 tccgggactg tcaattcagg tagttatgaa attgagatag aaaagttttt ccctcatata
     4981 agtgtatttc aggaagcatg cccaatgtgg gtgccgatgg tagaatacaa tgaatttgat
     5041 aaaccgggag ccgattattt tattcagcaa catatcaacc gtttattgaa taaatcggat
     5101 gaaatagata ccataatttt aggatgcacg cattaccctt tattgttgcc taaaattaaa
     5161 cagtatttac ctccgcatat taaagtaatt gcacagggtg aaattgttac agatagttta
     5221 atagattatt tacaacgaca tccggaaatg gataatatgt gcagtaaaag taaatcctta
     5281 tcgtttttta ctaccgattc taccgctatc ttcgacgaac acgctgcaat cttcttcggg
     5341 caggaagtgc attccgaaca tattcatatt taaaaagtaa aacacttagc gcaacttcgc
     5401 ggcacttagc gacttcgtgg taaactatgt attaattatt gttggaggta aagtagtttt
     5461 ttttcacaac aaaggctcaa aggggctcaa agtaacacga agaattttta tagagtagaa
     5521 atctgatgtt aaaaacaata aattatttct tttcagaatg acttattgcc tcatttaaat
     5581 aaggtataaa tgcatttaca tctgacggaa tactgaaggt tgatttattg gccaagccgg
     5641 ttaacgcttc ctgtaaatta aatttat
//
LOCUS       DAORKW010000206         5659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_567_length_5659_cov_4.504818, whole genome shotgun sequence.
ACCESSION   DAORKW010000206 DAORKW010000000
VERSION     DAORKW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_567_length_5659_cov_4.504818"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLB46_09135"
     CDS             <1..308
                     /locus_tag="PLB46_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76721.1"
                     /translation="FSKFFGGNKGQGGSGGGAGKAQAFIEKITADLNLNPEQVTKVEA
                     AIREFFMEKKQMKQGGDKDGLRESKQDFKDDILNALTPAQQQKFMDNIQTYKQLLKR"
     gene            403..933
                     /locus_tag="PLB46_09140"
     CDS             403..933
                     /locus_tag="PLB46_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76722.1"
                     /translation="MIDFITTSVLLFGSNEATIHLPEGLAKALIAAHSGWRYIILILL
                     VMAIIYGKQTASGKKAYEGATKKMGMFTMIAVDIQLLGGLVLYFFFIASQTNFKLGKL
                     KDQMGVSQFRSIAVEHAIGMLIALVLIHIGYSKAKKALNDTEAGKKQYVFYLIALIII
                     LLSIPWPFLHPERGWF"
     gene            complement(1024..1731)
                     /locus_tag="PLB46_09145"
     CDS             complement(1024..1731)
                     /locus_tag="PLB46_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRF76723.1"
                     /translation="MQNRIPTPDCEHCKSRGKSLFHFCHIEETDFINQNKTCALYKKG
                     QTIFHENAHAYGLYCLNAGKVKLYKQAPDGKEQILKIVTPGDFIGYGAMLSSNTYAVS
                     AEVIEDAVICFIPKDTVLKVFRENNRFSEGMVQLLTNTIDQTVEKMADIAYKPVRGRI
                     AEALLLLSESYKDEKNPEGVVSITREDLASYVGTVKETAIRVLKDLKDEGLIVTNNHT
                     ITILDPKGLTRVCELYD"
     gene            complement(1800..2765)
                     /locus_tag="PLB46_09150"
     CDS             complement(1800..2765)
                     /locus_tag="PLB46_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF76724.1"
                     /translation="MLEEELHSATGHYTTPMSNNKTAVKNMRKAEMPSPQINNILVPT
                     DFSENARKAVKIAVGIAEKHNAGLLLVHACHMPYTDEHMPPAMMQELILAQQNRCETQ
                     MVEFVATQKDLFGKLVPQTKVMMGFAAESILSLAHTENIDLIILGTQGVNGIEDKLFG
                     TVTWNIIKRSDIPVMAIPDVANDIQFKNIMIPFEGTAYDADIIQYLLKFAEKYNAVVH
                     GVHFIQDAPTYNKGVIDKLQNKFRKEIDAEKLQLHFPAEKNITEGIKKFASRNQIDMI
                     SMVTHNHGLFSTIFHMSVTRNIALYAQIPLLAYNMDRVQYETDND"
     gene            complement(2957..3505)
                     /locus_tag="PLB46_09155"
     CDS             complement(2957..3505)
                     /locus_tag="PLB46_09155"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HRF76725.1"
                     /translation="MKKLLLIVAIVAGYTANAQKSELTVPFFPIDEETKLVTYTDVIQ
                     VPGVVTDSLYNVAMAWMKTFYTSPSQAIKSQSKEEGIIEIKHQFQLTRKDKGQVVKAG
                     QINYYLTLQFRDGRFKYTVTKINLQGASYYGIENWINDEKFAKDETTVGYLVQIDEFM
                     QKLVGSLETEVRPAAPKKEEDW"
     gene            4072..>5659
                     /locus_tag="PLB46_09160"
     CDS             4072..>5659
                     /locus_tag="PLB46_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRF76726.1"
                     /translation="MEKEEAVVKNQSTYTAENIQVLEGLEAVRKRPAMYIGDTGFKGL
                     HHLVYEVVDNSIDEALAGYCRNISVAILPGNSIRVKDDGRGIPTELHKKENRSALEVV
                     MTVLHAGGKFDKDTYKVSGGLHGVGVSCVNALSTHLEAEVHRNGKIYKQEYSEGKPLY
                     AVKEIGDSDITGTYVTFKPDDTIFTVSEYKYETLAARLRELAFLNRGISLSLTDERVT
                     EEDGSHPTETFHSEGGLIEFVKYLDGTRVSIIPEPIYAEGSRDNVQVEIALQYNESYN
                     ENIHSYVNNINTIEGGTHVAGFRRALTRTFKSYGDREGMFEKAKVEISGDDFREGLTA
                     LISVKVPEPQFEGQTKTKLGNNEVMGIVDTISGEVLNNYLEENPKQARLIIGKVILAA
                     QARIAARKAREMVQRKSAFGGGGLPGKLADCTEKAAEKCELYLVEGDSAGGTAKQGRN
                     RTFQAILPLRGKILNVEKALDYKIFDNEEIKNIFTALGVMPAEDKTVDTAKLRYHKII
                     IMTDADVDGSHITTLILTFFF"
BASE COUNT         1695 a         1076 c         1088 g         1800 t
ORIGIN      
        1 tattttcaaa atttttcggc ggcaataaag gccagggcgg aagcggcgga ggtgccggta
       61 aagcacaggc ttttattgaa aaaattaccg cagacctcaa cctgaatccc gagcaggtta
      121 ccaaagttga ggccgctatc cgtgagtttt tcatggaaaa aaaacaaatg aaacaaggtg
      181 gcgataaaga tggattacgc gaaagcaaac aggactttaa ggatgatatt ctcaatgcct
      241 taacgccggc gcaacagcaa aaattcatgg ataatatcca aacctataaa cagctgttaa
      301 aacgctgata cattactgcc ggagagttgc cgctctccgg tattttttct cctcctctag
      361 gcaatttatt tattttttca cttaagatct aaatgcacac acatgattga ttttattact
      421 acgtccgtat tattattcgg atccaacgag gcaaccatac acttgcccga aggcctggca
      481 aaggctctaa tagcggctca ctccggttgg cgctacatta ttttaatttt actggtaatg
      541 gccattattt atggcaaaca aacagcatcc ggtaaaaaag cttatgaagg cgccactaaa
      601 aaaatgggta tgtttaccat gattgcggtt gatatccaat tgttaggagg tttggtgctt
      661 tattttttct ttattgcttc gcaaacaaat tttaagttgg gtaaattaaa agaccaaatg
      721 ggtgtctccc aattccgcag tattgctgta gaacatgcaa ttggcatgtt gatagcactc
      781 gtgttaattc atatcggcta tagcaaagct aaaaaggcct taaatgatac tgaagcaggt
      841 aaaaaacaat atgtatttta tttgattgcc cttattatta tcctgttatc tattccgtgg
      901 ccgtttttac acccggaacg tggatggttt taacggctat ctgctaaacg cattcaaaaa
      961 aataaagcgc atttatcatg aaaatccatg gttaatgcgc ttttgttata gtagctcaaa
     1021 aaattaatcg tataattcac atactctggt aaggcctttt gggtccagaa tggtaatggt
     1081 gtggttgttg gttacaatca acccttcatc cttcaaatct tttaaaacac gaatggctgt
     1141 ttctttaaca gtccctacat aagaggctaa atcctcgcgg gtaatgctca caacaccctc
     1201 agggttcttt tcgtctttgt aagattcact taacaacaat aaggcttcag ctatgcggcc
     1261 tctaaccggt ttataggcga tatccgccat tttttctact gtttggtcta tggtatttgt
     1321 taacaattgc accatacctt cagagaagcg gttattttct ctgaaaacct ttaacacagt
     1381 atcctttgga atgaaacaaa ttacggcatc ttcaatcact tcggcgctta cagcataggt
     1441 attggacgat aacatggcgc catatccaat aaaatcgccg ggtgttacaa tttttaatat
     1501 ctgttctttg ccgtccgggg cttgtttata caatttcact ttgccggcgt tcagacaata
     1561 aagtccgtat gcgtgggcat tttcgtgaaa aatagtctgc ccttttttgt ataaggcgca
     1621 ggttttattt tgattgatga agtctgtttc ttcgatatgg caaaagtgaa aaagcgattt
     1681 gcctctactt ttacagtgct cacaatccgg agtcggaatt cggttttgca tactcatatt
     1741 acaatatttt gcctgaaaaa attctcttaa aattaatcaa tttcttcgaa aagtcatgat
     1801 taatcattgt cagtctcata ttgcacgcgg tccatattgt aagccaataa cggaatttgt
     1861 gcataaagag caatatttct ggtaacactc atatgaaaaa tggtagaaaa caaaccatga
     1921 ttatgcgtta ccatactaat catgtctatc tgatttctag atgcaaattt tttgatacct
     1981 tcagtaatat ttttttctgc aggaaaatgt aattgtaatt tttccgcatc aatttctttc
     2041 ctgaatttgt tttgtaattt atcaataaca cctttgttgt aagtaggtgc atcctgaata
     2101 aaatgaacac cgtgcacaac agcattatat ttttctgcaa actttaataa atactgaata
     2161 atatcagcat cataagcagt gccttcaaat ggaatcatga tattcttaaa ctgaatatca
     2221 ttggccacat ccggaattgc cataaccgga atatcggaac gtttaatgat attccatgta
     2281 acggtaccga aaagtttatc ttcaatacca tttacaccct gagtacctaa aataattaaa
     2341 tcaatatttt cggtatgcgc taaacttaaa atagattcag ctgcaaatcc catcatcact
     2401 tttgtttgcg gaacaagttt gccaaataaa tctttttgtg ttgccacaaa ttcgaccatt
     2461 tgggtttcgc atctgttttg ttgcgccaaa atcaattcct gcatcatggc aggaggcata
     2521 tgctcatcgg tgtaaggcat atggcatgca tgcaccagca acaaaccggc attgtgtttt
     2581 tcggcaatgc cgacagcaat ttttacggcc tttcgagcat tttctgaaaa gtcggttgga
     2641 accaatatgt tgttaatttg cggggaaggc atttctgctt ttctcatatt tttaacagcg
     2701 gttttattgt tgctcatagg agtggtataa tgtccagttg cgctatgaag ttcttcttcc
     2761 agcataatct taaaatttgt aattcaaaaa tcacttaaat catttttttt agtcatgata
     2821 aggctcaaaa cgaatggtga tttttgtcat cgtttttatg aaaaattgtt attttgttaa
     2881 catatcgtaa cgataacagg caataaaaaa gcccttgcat gatgcaagag ctccgacata
     2941 gtagtggttg atttatttac cagtcttctt cttttttagg tgcggccggt ctaacttctg
     3001 tttccaggct tcccactaat ttctgcataa attcatctat ttgaacaaga tagccaacag
     3061 tggtttcatc cttggcaaac ttttcgtcgt taatccagtt ttcaatgcca taataagagg
     3121 caccttgtaa attaattttg gtaacggtgt atttaaagcg gccatctctg aattgtaaag
     3181 tgaggtagta gttaatctga ccggctttta ccacctgtcc tttatctttt ctggttaact
     3241 gaaactgatg tttaatttca atgataccct cttctttact ttggctttta atagcctggc
     3301 taggactggt ataaaatgtt ttcatccagg ccatagcaac gttatataac gaatcggtaa
     3361 ccactcccgg aacttgaatt acatcggtat aggtaaccaa tttggtttct tcatcaatcg
     3421 gaaaaaaagg aacggtaagc tccgattttt gggcatttgc agtatatcct gcaactattg
     3481 caacaataag caaaagtttt ttcatatgtt tttgtttaaa attttgtgac gaagatagtt
     3541 gtcaaaaatg ataccatttt ttccacttaa ctacttattc acattttatg catcaagggt
     3601 gtacttcact tggtaaatta cggctttttt atacctttgt gcaaaactga ataacatgac
     3661 ttcagcattc ttcatttcaa tagttgttat ttcttatgtg ctcgtagtga gcggtttgtt
     3721 tgtaattgaa tatatcaaaa gcaaacgcgg cggcaatacc ggtcaccact aattaatcct
     3781 atttcctatt cgtaacgtgc ctttgcgcga ttatttacct atttttcacc attagggtgt
     3841 ttttttttgc ttttcacacc atcgtcaaaa cggccctttt tttgtcattt ttttgcccgt
     3901 aattcagctg ttaaaataat gttgaaattc agtgtataaa atatgcttga aatacctgaa
     3961 aaacttatgt tttcagcgta taaaagtgta actttgtgct tcgtaaaata atattaaaaa
     4021 aagccctcgt ttttgatggc ttatacatgc tcagttttga ggtagcaaat tatggaaaaa
     4081 gaagaagcag ttgttaaaaa ccagtcgacc tacacggctg aaaatattca agtattagaa
     4141 ggtcttgaag cagttagaaa acgccctgcg atgtatatcg gtgataccgg ttttaagggc
     4201 ttacaccatt tagtgtatga ggtagtagat aactcgattg atgaggcatt agcaggttat
     4261 tgccgcaaca tcagcgtagc tattttaccc ggaaacagca tccgcgttaa ggatgatggt
     4321 cgtggtattc ctaccgaatt acataaaaaa gaaaaccgct cggcattaga agtggtaatg
     4381 acggttttac atgccggtgg taaatttgat aaagacacct acaaagtatc aggtggtttg
     4441 cacggtgtgg gtgtttcctg cgttaacgca ttatctaccc acctcgaagc tgaagtgcac
     4501 cgcaacggta aaatttataa gcaggaatac agtgaaggaa aaccattgta tgccgttaaa
     4561 gaaattggcg attctgatat caccggaacc tatgttacgt ttaaaccgga tgataccatt
     4621 tttaccgttt cagaatacaa atatgaaaca ctggctgctc gtctccgcga attggctttc
     4681 cttaacagag ggatttcact ttcacttacc gacgaacgtg taacagaaga agatggctca
     4741 catccaaccg aaacttttca cagcgaaggt ggtttaattg aatttgttaa atatttagac
     4801 ggaacccgcg tttctattat tcctgaacca atttatgcag aaggttcacg cgataatgtg
     4861 caagtagaaa ttgcactgca atacaacgaa tcctacaacg aaaatattca ctcgtatgtg
     4921 aataatatca ataccattga aggtggaaca cacgttgccg gtttccgcag agccttaaca
     4981 agaacattta aatcatacgg cgatcgtgaa ggcatgttcg agaaagcaaa agtggaaatt
     5041 tctggagacg attttcgcga aggtttaact gcgcttattt cagttaaagt tcctgaacca
     5101 cagtttgagg ggcaaaccaa aacaaagttg ggtaacaacg aagtaatggg tattgtggac
     5161 accatttctg gtgaggtgtt aaataattat ctggaagaaa atcctaaaca agctcgttta
     5221 atcatcggta aagttattct tgctgctcaa gccagaattg cagcgcgtaa agcacgcgaa
     5281 atggtgcaac gtaaaagtgc atttggtggt ggcggattgc cgggaaaatt ggccgactgc
     5341 accgaaaaag cggctgaaaa atgtgagtta tacctcgtcg aaggggatag tgcgggtggt
     5401 actgccaaac aaggccgtaa ccgtactttc caggccattt tgcctttgcg tggtaaaatc
     5461 cttaacgttg aaaaagcatt ggattacaaa attttcgata acgaagagat taaaaatatt
     5521 tttaccgcac tcggtgttat gccggctgaa gataaaacag tggatactgc aaaattgcgt
     5581 taccataaaa ttatcatcat gaccgatgcc gacgtggatg gtagtcacat taccacactt
     5641 attctaactt tcttcttca
//
LOCUS       DAORKW010000207         5649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_569_length_5649_cov_4.399893, whole genome shotgun sequence.
ACCESSION   DAORKW010000207 DAORKW010000000
VERSION     DAORKW010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_569_length_5649_cov_4.399893"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="PLB46_09165"
     CDS             <1..1578
                     /locus_tag="PLB46_09165"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76727.1"
                     /translation="ASKGLDDFWILKLDASGNISWQKTIGGSGTDMLVTIRQTSDGGY
                     IVGGSSISNASGDKTENSNGAYDYWIMKLTSTGTITWQNTIGGSDMDALSDVIQTNDG
                     GYLLGGYSSSGISGDKSEANLGSDDYWVVKIDASGNITWQNTIGGTGRDVLTGIHQSS
                     DNGYVIAGYTPSGISGDKTEATYGGFSYGDYWILKLDNSGNLIWQKTIGGNGEDLSGG
                     LDITPDGSIIISGTSMSPISGNKTTPFYGIADFWAIKLVANCTPTEEVCNAIDDDCNG
                     IIDDGVIIYATAIPLGPTTICQGNTVTFTADYNGDDIQWQRNGANITGATSMNYTANK
                     AGMYSCVTTNDCGEATSTAVNVTINKNPSAVITAAGPTSFCAGGSVTLNVTPVPGCTY
                     QWYKGGGLILGATGTAYVASTPGNYKCYVTKIATGCYKNSNSIPVTITCKENNNELQN
                     ELTVYPNPANQNIVILTNFIGENTITIIDAVGNSVMQMNTTATLLEINISNLPAGMYN
                     VLLQNTNGQTTTQFIKN"
     gene            1588..3477
                     /locus_tag="PLB46_09170"
     CDS             1588..3477
                     /locus_tag="PLB46_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76728.1"
                     /translation="MRKLSLILILTYCSANIFAQAPDIEWQNTIGGNSADLCYNALAT
                     SDGGFLLIGNSNSFISGDKTENNLGIAASYDYWVVKIDASGNVVWDNTIGGYKSEDLR
                     SLIETSDGGYLLGGWSDSDISVDKSENRIGGAGYGDYWVVKLDLDGNILWENTIGGTK
                     SDELYDIEETASGTYILGGTSYSPADFDKTIPSTNHDIWLVEIDNLGNVIWQKVIGGS
                     STDRFVEMEITSDGGLLIGASSLSGISGDKTEINHGGNDLWVIKLDAARNIQWENSFG
                     GTISDYMRDVIELPEGGYMILGASQSNMSADKSDNAFGVMNYNDFWLIKLDEDGNALW
                     DTIYGGDGEDYPASVMLSTDGNILLLGNSNSQISGNKTTNTYASTYDIWVIKITTNGV
                     KLWEKCMGGDSYDFPAEMVLANDGGVVIASQSYSTVSGDKTEANVGGASSDFWVIKLV
                     SEVCPAPVGLYADNITPNKATIHWDNITAAESYQIWYRPIGGGAWLKKSAPTNVKTIK
                     SLLPASTYEYKIRTSCNPGEYSEFSAVNNFTTLPFRTAVDLLETDYQVYPNPAINELV
                     ITGNFSGQTQLQIVDVTGKLVQQIICISNSNEQLLDVSSLPSGMYYITIKNSNTIQTI
                     QFIHQ"
     gene            3487..5364
                     /locus_tag="PLB46_09175"
     CDS             3487..5364
                     /locus_tag="PLB46_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76729.1"
                     /translation="MFQTKHAGKVLTVMLSTTLLFNNKLMNAQSPGIEWQGVYGGTAS
                     DYLTSSRQTADGGYIIGGYSNSGINGNKSEDKIGLYDYWIIKTDSTGNIVWQNVIGGT
                     QNDYLNNVEPTADGGYILGGTSFSDSTADKSENKNGAQDFWIVKTDASGNIVWENTIG
                     GDAYDYLHIIHQTPDGGYIAGGYSDSGLSGDKSEAGQGFIDYWIVKLDAFGNIEWDNT
                     IGGSGSDNLFDISLTSDGGYIVAGSSTSGISGDKTEAAAGNDYWILKLDGSGNIEWQN
                     TIGGSSEDIPASIVQTGDGGYIVGGKSNSAISGDKTEAAVGGNDYWVIKLDVSGNIEW
                     QNTIGGTGSDIVNKVMQASDGEYMIAGTSASPLSGDKTEASMGLDDYWLVKIDELGVI
                     TWDNTIGGNKSDNLVDVQVTSDNGYMLGGYSSTNVNGDKTVAGFGGIDFWNIKLLPEI
                     CPVPFGAFADNITAIKATVHWNPIPSIELFQIWYRPVGSPTWLKKTTTTNSKVLKSLT
                     PETTYEYKIRTKCLDGEFGEFTALNNFTTLPLREATMNERSFIEVYPNPANEVITIDI
                     ATVYENGVITICDMTGAIVKQLNITGSSEMVDISDIPSGFYIITMILDENVQAIQLIK
                     Q"
     gene            5388..>5649
                     /locus_tag="PLB46_09180"
     CDS             5388..>5649
                     /locus_tag="PLB46_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76730.1"
                     /translation="MKHASQFLKPLLFTLGITAAIQTASAQAPAIQWQKTIGSAGYDF
                     LNSIVQTSDGGYIMGGYSDGNSTGYKSEINYGGDDYWIVKVNA"
BASE COUNT         1890 a          927 c         1191 g         1641 t
ORIGIN      
        1 gctagcaagg gcctcgacga tttctggatt ttaaaattgg atgcttcagg caatatcagc
       61 tggcaaaaaa caattggagg aagtggtact gacatgttag tgactattcg ccaaactagt
      121 gacggaggtt acattgttgg cgggtcttca atatctaacg cttcaggcga taaaactgaa
      181 aattccaatg gggcttacga ctactggatt atgaaattaa cttcaacagg cacaataacc
      241 tggcaaaata ccatcggtgg aagtgatatg gatgctttaa gcgatgttat tcaaaccaac
      301 gacggcggtt atttattagg aggttattca tcttcaggaa ttagcggcga taaatcagaa
      361 gcaaatttag gctctgatga ttattgggtg gtaaaaatag acgcatcagg aaacatcacc
      421 tggcaaaata ctattggcgg cacaggacgt gatgttttaa ccggaataca tcaatcatca
      481 gataatggtt atgtaattgc aggttataca ccttcaggta tctccggaga taaaaccgag
      541 gctacctacg gcggattttc ttatggcgat tattggattt taaaattaga taacagcggt
      601 aatttaattt ggcaaaaaac aattggtgga aatggtgaag atttatccgg tggactagat
      661 attacacctg atggatcaat tattatttcc ggcacttcta tgtcgcccat atcaggaaat
      721 aaaacgacac cattttatgg tatcgccgat ttttgggcaa taaaattagt tgctaattgt
      781 acaccaactg aagaagtgtg taatgcaatt gatgacgatt gcaatggaat aattgatgat
      841 ggtgtgataa tttatgctac tgcaatacct ttaggcccta caactatatg tcagggaaat
      901 acagtaacat ttactgctga ttacaatggt gatgatatcc aatggcagcg caatggggca
      961 aatattacag gagcaacaag tatgaattat accgcaaata aagcaggtat gtatagttgc
     1021 gtaacaacca atgactgtgg tgaggcaact tccactgctg ttaatgttac tattaataaa
     1081 aatccaagtg cagtaattac agcggcggga cctacctcgt tttgtgcagg tggaagtgta
     1141 acattaaatg tcacaccagt accaggttgc acctatcagt ggtataaagg cggaggttta
     1201 atacttggcg caacaggaac agcttatgtg gcatctacac ccggaaatta taaatgttat
     1261 gtcaccaaaa tagcaaccgg ttgttataaa aattctaatt caattccggt gactattacc
     1321 tgcaaggaaa ataataacga attacaaaat gaattgactg tttatccaaa tccggctaat
     1381 caaaatattg ttatcctaac caattttatt ggtgaaaata ccattaccat tattgatgca
     1441 gttggaaata gtgttatgca aatgaatact acagcaacat tattagaaat taatatcagc
     1501 aatttacctg caggtatgta taacgtttta ttgcaaaata caaatgggca aaccaccaca
     1561 caatttatta aaaactaaaa taaatgtatg agaaaattat ccctcatttt aatattgaca
     1621 tattgtagtg caaacatatt tgcccaagca ccggatatcg aatggcagaa tacgattggc
     1681 ggtaactctg cagacttatg ttataacgct cttgcaacaa gcgatggtgg atttctatta
     1741 attggcaatt cgaattcatt tatttccgga gataaaacag aaaataattt aggtattgct
     1801 gcttcttacg attattgggt agtaaaaatt gatgcctccg gtaatgttgt ctgggataat
     1861 accattggtg gatataaatc ggaagattta cgcagtttaa tcgaaacttc tgatggtgga
     1921 tatttattag gcggatggtc cgattcagat atcagcgtcg ataaatctga aaacagaatc
     1981 ggtggtgccg gttatggaga ttattgggta gtgaaattag atttagatgg caatatcctt
     2041 tgggaaaata cgattggtgg aactaaaagt gatgaattat acgacattga agaaacggca
     2101 tccggaactt atattttagg tgggacttct tattcacccg ccgatttcga taaaacaata
     2161 ccttctacta atcatgatat ctggctggtt gaaatcgata atttaggtaa cgtaatttgg
     2221 caaaaagtaa ttggtggatc ttctaccgac cgatttgttg aaatggaaat aacttcagat
     2281 ggaggattat taattggtgc ttcatcttta tccggaataa gtggcgataa aacagaaatt
     2341 aatcatggtg gaaatgattt gtgggttatt aaattggatg cagctcgtaa tattcaatgg
     2401 gaaaattctt ttggcggaac aattagtgac tatatgcgcg atgttatcga attacccgaa
     2461 ggtggttata tgatacttgg tgcatcccaa tccaacatgt ctgccgataa atcagataat
     2521 gcatttggcg ttatgaatta taacgacttt tggctgatta aattagatga agacggtaat
     2581 gctttatggg ataccattta tggcggtgat ggggaagatt accctgcaag tgtaatgctg
     2641 tctactgatg gcaatatatt attacttggc aattcaaatt cgcaaattag cggaaataaa
     2701 acaactaata cctacgcaag cacttatgac atttgggtaa taaaaattac taccaatggc
     2761 gtaaaattat gggaaaagtg tatgggtggc gatagttatg attttccagc tgaaatggtt
     2821 ttagctaatg atggaggagt ggtaattgcc tctcaaagtt attcaactgt ttcaggcgat
     2881 aaaacagaag cgaatgttgg cggagccagc tcggactttt gggtaattaa acttgtttct
     2941 gaagtttgtc cggcacctgt tggattatat gcggataata ttacaccaaa taaagcaaca
     3001 atacattggg ataatatcac cgctgcggaa tcctatcaaa tttggtatcg accaattggt
     3061 ggcggtgcat ggctgaaaaa atcggcacca acaaatgtaa aaacaataaa atctttgttg
     3121 cctgcaagta cttatgaata taaaataaga accagttgta accctggtga atacagtgaa
     3181 ttttctgcag taaataattt caccacttta ccttttagaa cagctgttga tttacttgaa
     3241 actgattatc aggtttatcc aaatcctgcc ataaatgaat tagtaattac cggcaatttc
     3301 tccgggcaaa cacaattaca aattgtagat gtaactggaa aactagtgca acaaattata
     3361 tgcatatcaa atagcaacga acaattactg gatgtatcat cactaccttc cggtatgtat
     3421 tatatcacaa taaaaaattc aaacacaata cagactattc agtttattca tcaataacgt
     3481 tcaactatgt ttcagacaaa acacgcagga aaagtcttaa ctgtcatgtt aagcacaaca
     3541 ttgttattca acaataaatt aatgaacgct caatctcccg gcattgagtg gcagggagtt
     3601 tatggaggaa ccgcttccga ttatttgacc tcttcacgcc aaactgcaga tggcggatat
     3661 attattggcg gctattcaaa tagcggaatt aatggtaata aatcggagga caaaattggc
     3721 ctttacgatt attggataat caaaaccgat agcaccggaa atattgtttg gcaaaatgta
     3781 atcggcggaa cacaaaacga ttatttaaat aatgtggaac caacagctga tggcggatat
     3841 attttaggtg gtacttcttt tagtgatagc actgccgata aatcggaaaa taaaaatggc
     3901 gctcaggatt tctggattgt aaaaacagat gcttcaggaa atattgtatg ggaaaatact
     3961 atcggtggtg atgcatatga ttatttacac atcatacatc aaacgcctga tgggggatat
     4021 attgcaggtg ggtattccga cagcggacta agcggcgata aatctgaagc cggtcagggt
     4081 tttatcgatt actggattgt aaaattagat gcattcggaa atatcgagtg ggataatacc
     4141 atcggcggaa gtggctctga taatttattt gatatcagtt taacaagtga tggtggttat
     4201 attgttgcag gttcatcaac aagtggtatc agcggcgata aaacggaagc tgctgcagga
     4261 aacgattatt ggattttaaa attagacggc agcggaaata tcgaatggca aaataccatt
     4321 ggtggcagca gtgaagatat tcctgcaagt attgttcaaa caggagatgg tggttatatc
     4381 gttggtggaa aatctaactc tgcaatcagt ggtgataaaa cagaagctgc tgtaggcggt
     4441 aacgattatt gggtaattaa attagatgtt tcgggaaata tcgaatggca aaataccata
     4501 ggtggaaccg gttccgatat cgtaaataaa gtaatgcaag ccagcgatgg tgaatatatg
     4561 atcgctggaa cttctgccag cccgttaagt ggcgataaaa cagaagcatc aatgggttta
     4621 gatgattact ggttagtaaa aatcgacgaa ctcggtgtaa taacctggga taatactatc
     4681 ggtggaaata aaagcgataa tttggttgat gtgcaggtaa ccagcgataa cggttacatg
     4741 ttaggtggtt acagcagcac aaatgtaaat ggcgataaaa cggttgccgg ttttggtggt
     4801 atcgattttt ggaatatcaa attacttcct gaaatttgtc ctgttccttt tggtgctttt
     4861 gctgataata tcacggcaat taaagccaca gtgcattgga acccaattcc tagtatcgaa
     4921 ttattccaaa tctggtatcg tcctgttggt tcaccaacct ggttgaaaaa aacaacgaca
     4981 acaaattcta aagtattaaa atcattaaca cctgaaacta cctacgaata caaaatcaga
     5041 acaaaatgcc tggatggtga atttggtgaa tttactgcat taaacaattt taccacttta
     5101 ccattacgtg aagcaactat gaatgaacgt tcattcatag aggtgtatcc gaacccggcg
     5161 aatgaagtta tcacaattga tatcgctaca gtttatgaaa acggcgtaat tacaatttgt
     5221 gacatgaccg gtgccatcgt taaacaatta aatatcacag gttcttctga aatggtagat
     5281 attagtgata taccttcagg attttacatc ataacaatga tactggatga aaatgtccag
     5341 gcaatacaac taataaaaca ataatctaaa ataaaaaaaa acaataaatg aaacacgctt
     5401 ctcagttttt aaagccgctc ctgtttaccc taggtataac ggcagcaatt caaacggcat
     5461 cagcacaagc acctgccatc caatggcaga aaaccattgg tagtgcaggt tacgattttt
     5521 taaattcaat tgttcaaact tccgatggag gttatatcat gggaggttat tccgatggta
     5581 attccacagg atacaaaagt gaaatcaatt atggtggtga tgattactgg attgttaaag
     5641 taaacgcac
//
LOCUS       DAORKW010000208         5646 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_571_length_5646_cov_6.543910, whole genome shotgun sequence.
ACCESSION   DAORKW010000208 DAORKW010000000
VERSION     DAORKW010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_571_length_5646_cov_6.543910"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLB46_09185"
     CDS             <1..366
                     /locus_tag="PLB46_09185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76731.1"
                     /translation="PESGYPLKVLWVPAFSFGYVKVPIEAYYPPERVSGSINYSVNLY
                     PNPASNQVRLQAQCLQEDCEILNFMSIEVVNILGETTIKTTITNNGSLNISALPNGIY
                     KILVKFDDGSTVVKQLVKQ"
     gene            367..1263
                     /locus_tag="PLB46_09190"
     CDS             367..1263
                     /locus_tag="PLB46_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76732.1"
                     /translation="MKNNKFILTQYSALSACFLALNSSSIGQAVYTDIDPDIVLDNNW
                     ETAGVDMNNDGIVDFGFLNRSFDFSTYYSYYSSHLNALYAGPQSPNNEIAALTHVISP
                     SYGGFTVYFPFAINESNQIDESLIFHNEGYQTLAYRYIQTDGDYFPKGGIWYPEVLDH
                     YLGVRFYDSLDCLHYGWIRCDVFDNGRKLIVKDYAFENKCDTGIMAGDTIGDTSNVEI
                     SELDLIAPTIYSYSGTICINIDKQLLGANFSVFNFEGKVISSGCLNELNNKFNSAVEN
                     GTYIVAINKNSYSYSKQIILVQ"
     gene            1776..2993
                     /locus_tag="PLB46_09195"
     CDS             1776..2993
                     /locus_tag="PLB46_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRF76733.1"
                     /translation="MANVKLVLRKETKKDGTSPLAIRITKDRKSSYIYLEYSIKEDDW
                     DVKNQRVKKSHPNSTRLNNFLATKVAEALNRSLEIETERKDVSSKTVRQKIKPNGGAT
                     FFAQADLYIQNLEKDGKYNRYTADKPRVKHFKEFLKGQNIAFTDITIPLLERFKGYIK
                     TELKLSERSAINHLVVIRSVFSQAVKSEIVDRKYYPFGAGKISIKFPDSLKIGLTPEE
                     VYSIETIELAPGSFENHCRNLWLFSFYFAGMRISDVFRIKWSDIQNGRLFYSMGKNDK
                     GGSLKIPEKALSIIAQYKDDMQSVEDYIFPELKTLIDKQDKFIEQRRIMNMTSRVDKT
                     LQNVVAKRAGISKRLTMHIARHTFGNISGDRIPIQMLQKLYRHSSVTTTIGYQANFVN
                     KDADDALDMVINN"
     gene            3005..4006
                     /locus_tag="PLB46_09200"
     CDS             3005..4006
                     /locus_tag="PLB46_09200"
                     /inference="COORDINATES: protein motif:HMM:NF022633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HRF76734.1"
                     /translation="MDEQLELINKIEANFVNIIKAAFPQIFIRRWGSSGPHFDASFAT
                     ENQYFKNTEYYYNETTQFFHLTSIQNLFSIINSRNIRLYNLHNSSDPEEYSFAASQLE
                     LTQNQIDYRKSNIYTFSFCPFNDLSNIKIWETYGRNFTGAAIVFEIINDIEKWNNFHL
                     SKLHYSLSPKISQYVQDIKLLKQKYPYCTFYLNLEKLLGFHKTENWAEENEVRILTYS
                     PYDLQEERLKYIKKELKIENGRNRICEYINMPLWVDNSSRYLECSEANLNLDRRQTLP
                     TDDFFIKQPKIKINTILFGQNCGLTPLEYRKFKFEIENMFKYNYGYNLKMDENMYHC"
     gene            complement(4003..4551)
                     /locus_tag="PLB46_09205"
     CDS             complement(4003..4551)
                     /locus_tag="PLB46_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76735.1"
                     /translation="MNLKNKNIYKTLAFVIFTAIPTFPFYLNFFTNEKAVNTTLLGLN
                     NNSFNLFALIYPCFVGLVVWKYLKLVEQITRIKNAIFELMKHDNLEGKVRLECDNSLE
                     MALRKSNDVWSELLKDTLTKDKQVLQKIALIQKFSSTRDSDGIDLAKKYLDRHNELFV
                     NILNELDAPNEYYKNLQAKASS"
     gene            4655..5260
                     /locus_tag="PLB46_09210"
     CDS             4655..5260
                     /locus_tag="PLB46_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76736.1"
                     /translation="MKEKDTKAIERALRGLPPSLLLPIGARDESSIIKYIGSIERTLV
                     QGVPNKAFIVNVEQHGEINNKLAIWKHPESSALHQCLQVWVHVDYKQYRQVYKMAFPG
                     VDLTNKVVDHILNRRVARLKGMNYIRLLPISRAANSSSGSVSEQYGYEYFMSKEMQEI
                     YKDIYVQYADLADIVKMLDIKTGGSFQEGVNEAQKYLLEYD"
     gene            complement(5297..>5646)
                     /locus_tag="PLB46_09215"
     CDS             complement(5297..>5646)
                     /locus_tag="PLB46_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76737.1"
                     /translation="EIREFSKARKIFYSEKFDFLEENNAIKELFIFFINNKNSNGLLN
                     TIRNITPLDLDPALANDYIQALIHPHMLQEHLDEIEANLENIPDKLDRIEVMELLGDL
                     RSQFIDGDEEEID"
BASE COUNT         1988 a          853 c          975 g         1830 t
ORIGIN      
        1 cctgaaagtg gatatccgtt aaaagtttta tgggtgcccg ctttttcatt tggttacgtt
       61 aaggtgccaa ttgaggcgta ttacccgccg gaaagagttt caggttcaat taattatagc
      121 gtcaaccttt atccaaaccc agccagtaat caagtccgtt tacaagcaca atgtttacag
      181 gaagattgtg aaattttaaa tttcatgtca attgaagtag tcaatattct tggtgaaaca
      241 acaataaaaa ctactattac aaacaatgga agcttaaata tttctgcttt gcctaatgga
      301 atttacaaaa ttttggttaa atttgatgat ggaagcacag tagtaaaaca acttgtaaaa
      361 cagtaagtga aaaataacaa atttatatta acacagtatt ctgctttatc agcttgcttt
      421 ttggctttaa attctagctc gataggacaa gctgtatata cagatattga tcctgatata
      481 gttttagaca ataattggga aacagcaggc gttgacatga ataatgatgg tatagttgac
      541 ttcgggtttt taaatcggtc gtttgatttt tctacttact attcttacta tagttcacac
      601 cttaatgcat tatatgctgg tcctcaatcc ccgaataacg aaattgctgc acttactcac
      661 gttataagtc catcatatgg tggctttacc gtgtatttcc catttgcaat aaatgaaagt
      721 aaccaaattg atgaaagctt aatttttcat aatgaagggt atcaaacgtt ggcatacaga
      781 tatattcaaa cggatggtga ttactttcca aagggaggaa tctggtatcc cgaagtgcta
      841 gaccactatc ttggtgtcag attttatgat tcattggatt gtttacacta tggttggata
      901 cgctgcgatg tgtttgataa tggaagaaag ctaattgtta aggactacgc atttgaaaat
      961 aaatgtgata caggaattat ggccggtgat acaataggtg ataccagcaa tgtcgaaatt
     1021 tccgagctag atttgatagc gccaacaatt tatagttact cggggactat ttgtataaat
     1081 attgacaaac aactcttggg agccaacttt tctgtattta attttgaagg gaaggtaata
     1141 agttcaggct gcttgaatga attaaataat aaatttaatt cggctgtaga aaatggaaca
     1201 tacattgtag ccattaataa aaattcatat agttattcaa aacaaattat actggttcaa
     1261 taaacaaacc ggtatattaa aaaaggaggc gttgtatgca tagcgcctcc tttcttttta
     1321 actatttccc gaaattttta aacactgcac gttataaaaa ataacattct ctgttgtagt
     1381 aaatgtatat agagtataag tctaggtttc attctcaaaa aagtatcttg tagttaatat
     1441 accttcaata atttccagtt tatcttgttt ggttggaata tccatccata tctcaaatat
     1501 actaaacttc aataaatcac aaatagttgt aactatgttg caccaaaaca aaaaggcttc
     1561 gaagaatttc tcttcaaagc ctttttttct gggtggtgtg gggcgggatc gaaccgccga
     1621 cacaaggatt ttcagtcctt gttataatgt attaatatgg tttaatacgg cctgaaagta
     1681 ttgattatat tgaattaacc caaaattaat ttcataaaaa tacataataa accgcaaaat
     1741 gttgtagtat tgttgtagta aataattcta caataatggc aaatgtaaaa ctcgttttac
     1801 gcaaggaaac aaagaaggac gggacttccc cacttgctat aagaattaca aaggatagga
     1861 agtcttcata tatttattta gaatatagta tcaaagaaga cgactgggac gtaaaaaatc
     1921 aaagggtaaa gaagagtcat ccgaactcaa ctcggcttaa taattttctg gcaacaaaag
     1981 ttgcagaggc actaaatcgg tctttggaaa ttgaaacaga gcgaaaggat gtttcttcca
     2041 agacagttcg tcaaaaaata aagcctaatg gtggggctac tttttttgca caagcggatt
     2101 tatatattca aaaccttgaa aaagacggaa aatacaacag atatactgct gataagcctc
     2161 gtgttaaaca cttcaaggaa ttccttaaag gacagaatat tgcatttacc gatattacta
     2221 taccactatt ggaaagattt aaaggatata tcaaaacaga attaaagctg agtgagcgaa
     2281 gtgctattaa tcacttagta gtaatacggt cagtatttag tcaggctgta aaaagtgaaa
     2341 ttgtagatag aaaatattac ccttttggtg caggtaaaat aagcattaaa tttccggatt
     2401 cattaaaaat tgggcttacc cctgaagaag tatattctat tgaaacaatt gagttagctc
     2461 cagggtcttt tgagaatcat tgtaggaatc tatggctatt ttcattctat ttcgctggaa
     2521 tgagaatttc tgatgtattt agaattaaat ggagtgatat tcaaaacgga aggttatttt
     2581 actctatggg taaaaatgat aaaggcggaa gtctaaaaat accagaaaaa gcgctaagta
     2641 taattgcgca atataaagat gacatgcaaa gtgttgaaga ttacatcttt cccgaactaa
     2701 aaaccctcat cgacaaacaa gataaattca tcgaacaaag gcgcataatg aatatgacca
     2761 gccgggtgga taagacgcta caaaatgtcg ttgcaaaacg agcgggaatt tccaagcggt
     2821 taacaatgca tatagctaga catacctttg gtaacatttc gggggatagg atacctattc
     2881 aaatgcttca aaagctatat aggcactctt cagttactac aactatcggt tatcaagcta
     2941 attttgttaa taaagatgct gatgatgctt tggatatggt gataaacaat taaattaact
     3001 aaaaatggat gaacaacttg aacttataaa taaaattgaa gctaattttg taaatataat
     3061 taaggcagca ttccctcaaa tttttataag gcgctgggga tcttctggtc cacattttga
     3121 tgcgagcttt gcaactgaaa atcaatactt taaaaataca gaatactact ataatgaaac
     3181 cacacaattt tttcatctta cgagcataca aaatctattt tcaataatta attcacgaaa
     3241 cataaggtta tacaatcttc ataactcaag tgatcctgaa gagtatagtt ttgccgctag
     3301 tcagttggaa ttaacccaaa atcaaatcga ttatcgtaaa agcaatattt atacgttttc
     3361 attttgtcca tttaatgatt taagtaatat aaaaatttgg gaaacttatg gacgcaattt
     3421 tacaggagcc gcaattgttt tcgagattat taatgatata gaaaagtgga ataattttca
     3481 tttatctaaa cttcactata gcttaagccc caaaatttca caatatgttc aagatattaa
     3541 actattaaag cagaagtatc cgtattgcac cttttatttg aatttggaaa aattacttgg
     3601 gttccataaa acagaaaatt gggctgaaga aaacgaagtg cgtattttga cttattcacc
     3661 ctatgattta caagaggaaa gattgaaata tattaagaag gaattaaaaa ttgaaaatgg
     3721 aagaaaccga atttgtgaat atattaacat gcccttatgg gtagacaact cctcaaggta
     3781 tttagagtgt tcagaggcta acttaaattt agatagaaga caaactcttc ccacagatga
     3841 tttttttatc aagcaaccga aaattaaaat caatactatt ttgtttggtc aaaactgtgg
     3901 tttaacgccg cttgaatata gaaaattcaa atttgaaatt gaaaatatgt ttaaatataa
     3961 ttatggctat aatttaaaaa tggatgagaa catgtatcat tgctagctcg aggctttggc
     4021 ttgtaagttc ttataatact cgttaggagc atccagttcg tttaaaatgt ttacaaatag
     4081 ctcattgtgt ctatctaaat atttttttgc caagtcaata ccatcactat ctcgtgtgct
     4141 tgaaaatttc tgaatcaatg caattttttg taaaacctgc ttatccttgg ttagcgtgtc
     4201 tttaagtaat tctgaccata catcattact ttttcttaaa gccatttcaa gggaattatc
     4261 acattccaat ctgaccttcc cttcaaggtt atcatgtttc atgagttcaa atattgcatt
     4321 cttaattcgt gtaatttgct cgactagttt taagtatttc caaacaacca atcctacaaa
     4381 acaaggatag attaaagcaa acaaattgaa tgaattatta tttaatccaa gaagtgtagt
     4441 attaacagcc ttctcattgg taaaaaaatt taagtagaat ggaaaagttg gaatagctgt
     4501 aaatattaca aacgctaaag ttttataaat atttttattt tttaaattca tttggattta
     4561 ttttagatta tgttaaaaat attttaggaa ggtcaatttt ttgtaaaagt cacaaaattg
     4621 tttttaaatt taaaatttaa atgcaaaata atccatgaag gaaaaagaca cgaaagcaat
     4681 tgaaagagcc ctgcgagggc tgccacccag tctattatta cctataggag ctagggacga
     4741 gagttcaata ataaagtata ttggttcaat agaaagaaca ttagttcaag gggttccaaa
     4801 taaagctttt attgttaatg tagagcaaca tggagaaatt aataacaaac ttgccatatg
     4861 gaagcaccca gagtcatcag ctctacatca atgtttacag gtatgggttc atgttgatta
     4921 taaacagtat aggcaagtat ataaaatggc ctttccaggt gtagacttaa ctaataaagt
     4981 agtagaccat attttaaatc gcagagttgc acggcttaag gggatgaact atattagact
     5041 tttaccaata tctagagccg cgaattcaag cagcggttca gtttccgaac agtatgggta
     5101 tgaatatttt atgtcaaaag agatgcaaga aatctataaa gatatatatg ttcaatatgc
     5161 tgacctagct gatattgtta aaatgctaga tataaaaacc ggtggctcat ttcaagaagg
     5221 tgttaatgag gcgcaaaaat acttattaga gtatgactag aattgggttt ttacatgaaa
     5281 ttacaggttt taaaagttaa tcaatttctt cttcatcgcc atcaataaat tgactcctta
     5341 aatcacctaa cagttccatt acttcaattc tatctagctt gtccgggatg ttttcaaggt
     5401 ttgcttctat ttcatctaag tgctcctgca acatgtgcgg gtgaatcaat gcttgaatat
     5461 aatcattagc aagcgctgga tctaagtcta aaggagtaat atttcttatt gtatttaata
     5521 gaccatttga atttttgttg tttatgaaaa atataaatag ttcttttatt gcattatttt
     5581 cctccaaaaa gtcgaatttt tcggaataaa atatttttcg cgcttttgaa aactctctta
     5641 tttcat
//
LOCUS       DAORKW010000209         5546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_589_length_5546_cov_5.862502, whole genome shotgun sequence.
ACCESSION   DAORKW010000209 DAORKW010000000
VERSION     DAORKW010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_589_length_5546_cov_5.862502"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="PLB46_09220"
     CDS             complement(<1..1593)
                     /locus_tag="PLB46_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76738.1"
                     /translation="MKKRFTPIAMLLLAFSFTSAVSAQSWGFAQTGNWAYDIAVGPEG
                     TMFAAGLAGDDPAWLDVTSGYVTKLDANGEQLWSYAPTGFPWVSGNANSVMPTADGGA
                     AVYYAAAGGSQEIVKLDDAGAVEWTTSSWDGEFYVYGGQAVLLNDGRYVIGGLADFEH
                     KFYEVGTDGSLLSTWTVAPDTGAAWTWSYFTYKEAGITATADGGFAYATGNEGNKIVY
                     KYDSDLNLEWSSYIPWDAELNWEYAYQNGLKTSTDGGFILSGSGTTPDGNYNGVVRKL
                     DSDGNVDWTTLLFHGSPYEEGAYAVETGDGNVIAFSQDGGELSVYGWILDGTTGAEIG
                     ETFLPIMATPGGFSDTGLEVWDIESTLDGGYAIAGRMWLEDFDQRFTVVKSNADGTFD
                     DCIFNCVWPGDANNDGYADGSDLFEIGINYGATGTPRADMTIDWSGKLAQAWMEPDSV
                     YWYILNDLKWTDCDGNGTINDDDTTAVINNMGLDHPLNNARLEGGEAPLFFEPEMDVL
                     HVGLNEIQIYLGDDLTSLDEFDG"
     gene            complement(1787..3976)
                     /locus_tag="PLB46_09225"
     CDS             complement(1787..3976)
                     /locus_tag="PLB46_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76739.1"
                     /translation="MKNILLFSSLFAIATVATAQDPWVYAKQATEAWPGATAKAALLV
                     DDGVLSVGEIGLLDIMEGELYMVKTDMAGNVLWEQSHDKPGDMEVVNSVSALPDDGGY
                     VVASLLFSEFRPWFIKTDVNGNLVWESSAWSDGLPENSATRTFAYSLPSGDIACVVAN
                     DVFRQLDIYTVDNATGNLIATTTHLYEDIIGLSLFSASTSDITQTADGGFLFATRFGV
                     VDTFYNQLTKIDAGLNVEEGNPIGMAVDNFFPAHININSDGSVNVSGAKNIGFFGDNY
                     QAAIATFNAAYDYTGMEIISGGLLHSNCGEITQTPSGNYKLIRYNYSGDFFDMAVGDY
                     VEVVNLNASFVETDATTFNYAPYNTLEFITATADEHYITGGMAWTIGEPNYFLVIASG
                     AGDEVPDCIFNCVWPGDADNSSLTDMDDILALGLGYGATGDPRDDMSIGWYAHNANGW
                     ATELPSGVNHKYTDCNGDGIINDDDTIAISNNFSFDHAVYTLKTTEGDLPLTFAPTGP
                     LSIGLNTIPIVLGDALNSADAIYALRFNIVVESENIDATTIKVQFNDSFLGAVNDLIY
                     LSKTNGDAATVHAAVAKTNQTNASGYGEIGTLSFVVIDNIAGKTEVDNVLLSFENVKA
                     IDVTNTELSILSTNASIESPTAIQTINSNEFTISPNPVSGNKIHITTNEPILAIALYN
                     SYGEKVSFSGELNTKTLTLPVLPMGQYLLQIETDKGLSSQKLVILGR"
     gene            4143..>5546
                     /locus_tag="PLB46_09230"
     CDS             4143..>5546
                     /locus_tag="PLB46_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76740.1"
                     /translation="MALPKVISIYSSLAAKERDRFGLFVRSPFHNTRNKIIGLIEHIE
                     HLSANLKDEQEFDTIWDKKAAHQKIFTGKKYNELQLNNLLSDLYQLLEKFLAWQTFEK
                     NEEQQNVMVIENLMETSVMDNAGKLIKQQTLQKSKDAIAGFKLHQLADQYYFQRSRKG
                     DNAYLLKGQETLDIFYLSNQLRIWCELLSRSNILALQYDAYKFHRFIDYLENNLQQYG
                     EQPTISIYYPILLWLKDQHNDAWYLGFREKLFLHIGEFPEQEAKDIVAYVQNYCVKRI
                     NEGRNEFLHEWLEISRFMLPLNLLQEGQHISEWTYKNIVTAGVRLQEFVWTEQFIHQY
                     YNQLAPEGRDNAYQYNLAVLYYEKRDFNRAMQLLNKVHFTDPNYYLDAKSILLKIYYD
                     HHEYDAILSLRDTVKIYLLRDKLLSKNQRSLYKSLFNNTIKLYKLRLEKGVISNEKWH
                     LAFQSLKKDVLESSLVAN"
BASE COUNT         1813 a         1148 c          904 g         1681 t
ORIGIN      
        1 accatcaaat tcatctagtg aagttaaatc atcacctaaa taaatttgaa tttcatttaa
       61 accaacgtgt aaaacatcca tttcaggttc aaaaaataac ggtgcttcac caccttctaa
      121 acgcgcgttg tttaatgggt gatcaagacc catattattg ataactgcgg tagtatcatc
      181 atcattaatt gttccgttac catcacaatc ggtccatttt aaatcgttga gtatgtacca
      241 ataaactgaa tccggttcca tccaagcctg agcaagttta ccactccagt ctattgtcat
      301 atcggcacga ggagttcctg ttgcaccgta gttgatacca atttcaaata aatctgagcc
      361 atcagcataa ccatcgttat tggcatcacc cggccaaacg caattgaaaa tacaatcgtc
      421 gaatgttcca tcagcattag atttaactac tgtaaaacgc tggtcaaaat cttctaacca
      481 catacggcct gctattgcgt aaccaccatc aagtgtgctt tcaatatccc aaacttctaa
      541 acctgtatct gaaaatccac caggtgtagc cataattggt aaaaatgttt caccaatttc
      601 agcacctgta gtgccatcta atatccagcc atatacactt aattcaccgc cgtcctgact
      661 aaatgcaata acatttccgt cacctgtttc aactgcatat gcaccttctt cgtatggtga
      721 accatggaat aataaagttg tccagtcaac attaccgtcg ctatctaatt ttcttaccac
      781 accattgtaa tttccatcgg gagtagttcc ggagccggat aaaataaatc cgccatcagt
      841 tgaagttttt aaaccgtttt ggtaagcata ttcccaattt aattcagcat cccatggtat
      901 ataactagac cattctaagt ttaaatctga gtcgtatttg taaacaattt tattgccttc
      961 atttccagtt gcataagcaa aaccaccatc agcagttgca gttatacctg cttctttgta
     1021 agtaaaataa ctccacgtcc acgctgcacc agtatcagga gcaacagtcc aggtagataa
     1081 taaactacca tctgttccta cttcataaaa tttatgttca aaatcagcta aacccccaat
     1141 tacataacgg ccatcattca acaaaaccgc ctgtccgcca tacacataaa attcaccatc
     1201 ccaagatgat gttgtccatt caacagcacc tgcatcatct agttttacaa tttcctgtga
     1261 gccaccggca gcagcataat atacagcagc gccgccgtca gcagttggca ttacgctgtt
     1321 agcgttgcct gaaacccatg gaaaaccagt tggtgcatag ctccataatt gctcaccatt
     1381 cgcatctaat ttagtaacgt aaccagaagt tacatctaac caagccggat catcacctgc
     1441 taaaccagct gcaaacatag ttccttccgg accgactgca atatcatagg cccaatttcc
     1501 ggtttgtgca aatccccaac tttgagctga aactgctgag gtaaaagaaa aagccaagag
     1561 gagcatggct atcggagtaa atcgtttttt cataataaat atgtttaagt gtatctgaga
     1621 caattttaca gatacaaata tcatctaccc caaagctaat taaaagaaca taaaatcacc
     1681 cttatagctt gaaaataaag cattattctg cataaaatgc tgatttacaa cattttatgc
     1741 agaataaaac atacgattta tagattttta tgtgctaatt ataattttat cggcccaaaa
     1801 tcacgagttt ttggctgctt aaacctttgt ctgtttcaat ttgtaacaaa tattgaccca
     1861 ttggaagcac cggtagcgta agtgttttag tattcaattc tccactaaac gatacctttt
     1921 ccccgtaact attatataaa gcaatagcaa gtatcggttc attggttgta atatgaattt
     1981 tatttccact aaccgggttt ggagaaatag taaattcatt tgaattaata gtttgaattg
     2041 ctgttggcga ttcaatagat gcattagttg acaaaatact taattcagta ttggtaacat
     2101 caattgcttt tacattttcg aaacttaata atacattatc aacttctgtt tttccggcaa
     2161 tgttgtcgat aactacaaaa cttaatgtac ctatttcacc atatccggat gcattggtct
     2221 gatttgtttt tgcaacagca gcatgaaccg tagcagcatc accatttgtt tttgataaat
     2281 aaattaaatc atttactgct cctaaaaacg aatcgttgaa ttgcacttta atagtagttg
     2341 catctatgtt ttcgctttcc acaacaatat taaatctcaa cgcgtaaatt gcatctgcgc
     2401 tgtttaatgc atcgcccaat acaattggaa ttgtatttaa tccgatactc agcggaccgg
     2461 tgggtgcaaa agttaatggc aaatctcctt cagttgtttt taatgtatat accgcatggt
     2521 caaaagaaaa attattgctt attgcaatgg tgtcatcatc attaataata ccatcgccat
     2581 tgcagtctgt atatttatga ttaacacccg atggtaattc ggttgcccag ccgtttgcgt
     2641 tatgtgcata ccagccaata ctcatatcat cacgcgggtc gccggttgcg ccgtatccca
     2701 aacccagcgc cagtatatcg tccatgtctg ttaaactact gttatccgca tcaccaggcc
     2761 aaacacaatt aaaaatacaa tcgggaactt catctcctgc acctgatgct atcactaaaa
     2821 aataattagg ttcaccaatt gtccatgcca taccaccggt tatataatgc tcatctgcag
     2881 tagctgtaat aaattctaat gtattatatg gtgcataatt aaatgttgtg gcatctgttt
     2941 caacaaacga tgcatttaaa ttaactactt caacataatc accaacggcc atatcaaaaa
     3001 agtcgccgga ataattatat ctgattaatt tataattacc ggaaggtgtt tgtgttattt
     3061 caccgcaatt agaatgtaat aatccgcctg aaattatttc cataccggta taatcgtaag
     3121 cggcattaaa tgttgcgatg gctgcctgat aattatctcc aaaaaaaccg atgttttttg
     3181 caccacttac atttacactg ccatcactat ttatattaat gtgtgcagga aaaaaattat
     3241 cgacagccat tccaattgga ttgccttctt caacatttaa tccggcatct atttttgtga
     3301 gttgattata aaatgtatcc accacaccaa aacgtgttgc aaataaaaat ccgccatcag
     3361 ctgtttgtgt aatatctgag gttgatgcag aaaatagcga caacccgata atgtcttcat
     3421 ataaatgtgt agttgttgca attaaattac ctgttgcatt atcaaccgta taaatatcga
     3481 gttgacgaaa cacatcgttt gctaccacac aagcaatatc acctgatggc aaactataag
     3541 caaaagttct tgttgcggaa ttttcaggta aaccatccga ccaggccgaa ctttcccaaa
     3601 ctaaatttcc gtttacgtca gtttttataa accatgggcg gaattcgcta aataataaag
     3661 aggcgaccac gtaaccacca tcatccggca gcgctgagac actgtttacc acttccatat
     3721 ctccgggttt gtcgtgcgac tgctcccaca acacatttcc agccatgtcg gttttaacca
     3781 tatacaactc accttccata atgtcgagca ggccaatttc acctaccgac aaaacgccat
     3841 catccaccaa taatgcggct tttgcagtcg cacccggcca ggcttctgtc gcttgttttg
     3901 cataaaccca tggatcctgc gcagtcgcta ctgtagcaat tgcgaaaagg gaactgaata
     3961 gtagaatatt tttcaccttc ataaaattaa gtgtttggtt ctcaatagca caaaagtgta
     4021 ggcaaaaaat tactttgaga attacaaaat tgctattttt atagccgcaa tctaatagct
     4081 atattttcat tatttcattg tatatcaata tgttatgaat tcattaatat caattttata
     4141 gcatggcatt acccaaagtt ataagcattt acagttcgtt agcagctaaa gaacgggacc
     4201 gatttggtct atttgtccga agtccttttc ataatacccg caataaaatt attgggttga
     4261 ttgaacatat cgaacattta agcgctaacc ttaaagatga acaggaattt gataccattt
     4321 gggataaaaa agcagcgcat caaaaaatat ttaccggtaa aaaatataat gaattacaac
     4381 tgaataatct actgagtgat ttataccagt tattagaaaa gtttttagcc tggcaaacat
     4441 ttgaaaaaaa tgaggagcag caaaatgtaa tggtgataga aaatctgatg gaaacatctg
     4501 taatggataa tgcaggaaaa ttaattaaac aacaaacctt acaaaaaagt aaagatgcca
     4561 ttgcaggatt taagttacat cagttagcgg accaatatta ttttcaacgt tccagaaaag
     4621 gagataatgc ttatttatta aaagggcagg aaacgctcga tatattttat ttaagcaatc
     4681 aattgcgtat ctggtgcgag ttactgagtc gttcaaatat attagcactg caatatgatg
     4741 cctacaagtt tcatcgcttt atcgattacc tggaaaataa tttacaacaa tacggtgagc
     4801 aacccaccat cagcatttat tatcccattt tattgtggtt aaaagaccag cacaacgacg
     4861 cctggtattt gggtttcagg gaaaaactct ttttacatat tggcgaattt cccgaacagg
     4921 aagcaaagga tattgtggct tatgtccaaa attattgtgt taaacgcatc aacgaagggc
     4981 gcaatgaatt cctgcacgaa tggctcgaaa tttcacggtt tatgttgcct ttaaatttat
     5041 tacaagaggg acaacatatt tcggaatgga cctataaaaa tattgtaact gcaggtgtac
     5101 gattacagga atttgtctgg accgaacaat ttattcacca atattataat cagcttgcgc
     5161 cggaaggaag agataatgct tatcaatata atcttgctgt actctattat gaaaaacgcg
     5221 attttaaccg cgccatgcaa ttattaaaca aggtgcattt tacagacccc aattattatc
     5281 tcgatgcgaa aagtatttta ttaaaaatat attacgacca ccatgaatac gatgcgattt
     5341 tatcgttgag agatacagta aaaatttatt tactcagaga taaattattg agtaaaaatc
     5401 aacgctcatt gtataaaagt ttatttaaca acacgattaa actgtataag ctgcgtttgg
     5461 aaaaaggtgt aataagcaac gaaaaatggc atctcgcctt ccaatcactg aaaaaagatg
     5521 tattggaaag tagcctggtt gccaat
//
LOCUS       DAORKW010000210         5534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_592_length_5534_cov_5.541340, whole genome shotgun sequence.
ACCESSION   DAORKW010000210 DAORKW010000000
VERSION     DAORKW010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_592_length_5534_cov_5.541340"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..897)
                     /locus_tag="PLB46_09235"
     CDS             complement(112..897)
                     /locus_tag="PLB46_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF76741.1"
                     /translation="MIRLLKIELLKLVPYRTFKILFAIYLLLMTLVLLVGKNLGGSGD
                     EGLTSANVLGFPNIWNYYTLYACYFNIILAILVIFVTCNEFTYRTLRQNIIDGLTRQE
                     AVIGKITLIVLLSIITTVILIISGLVAGNIYSLPEDRDQMFGHSGFIFAYFIQSLGMM
                     SMGYFFGTLFKRTGLAVIIFLIFLFPIDVILREGIFPDGFGDYMPVATYFLKMVPFPL
                     DHTMSGGLEPTPVAPTALSLAVGAAYAVIFCGLGWWITKKRDL"
     gene            complement(904..1794)
                     /locus_tag="PLB46_09240"
     CDS             complement(904..1794)
                     /locus_tag="PLB46_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF76742.1"
                     /translation="METVLSTTALKKQYGPIKAVNGLDLRVEKGAVYGILGPNGSGKT
                     TTLGMLLGIIQPTSGAYSWFNNGSLDINRKRIGSLLEQPNFYPYLSGEQNLKVVAKIK
                     GVDEGTIDAVLASVNLLERKKSPARTYSLGMRQRLALASALLGNPDVLLLDEPTNGLD
                     PQGIAEVREFITQQSAQGRTIILASHILDEVEKVCTHVSVIKKGTLLAEGSVADLIKG
                     ETQVEIAADNNEIVLQAIQSFTGIKKAAQENSMIIINVDEKFNAAQLNKYLSEKNIYL
                     QHLNVRKKRLEEQFLELTAE"
     gene            1968..2570
                     /locus_tag="PLB46_09245"
     CDS             1968..2570
                     /locus_tag="PLB46_09245"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRF76743.1"
                     /translation="MLQATYQDELIEAGCDEAGRGCLAGPVVAAAVILPKNFFHPLLN
                     DSKQLTENERTLLAPIIKAAAIDWKIGICDNHEIDAINILKASFKAMHKAVDQMKKKP
                     GLLLIDGNRFYKYPRIPHICIVKGDAKFMSIAAASVLAKTYRDELMHKLHNEFPIYNW
                     QQNKGYPTKDHRKAIAQFGSTNYHRKTFRLLPENEVTLFE"
     gene            2672..3907
                     /locus_tag="PLB46_09250"
     CDS             2672..3907
                     /locus_tag="PLB46_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRF76744.1"
                     /translation="MIAFQTFKLDNGLTVIVNEDNTTQLCAVNVLYKVGARDEIITKT
                     GFAHLFEHLMFGGSANIPDFDTELQLAGGTNNAYTSNDLTNYYDILPVSNIETALWLE
                     SDRMLKLDFSEKSLNVQRNVVCEEFKEHYINKPYGDVWHILRKLVYEKHPYQWPTIGL
                     ELQHVQDATLTDVENFFYKYYRPNNAILTISGGISADKALQLAKKWFGDIPSGETFNR
                     NIPDEPEQQTAKFLHVKKDVPVNVIYKAWKMPGRTEPQYYATNLLSDVLGLGESSRLQ
                     HALKKETKLFTDIAAYITGSIDTGMFIITGKLAEGVTYEQAEKGIQQELDKIRQAPVS
                     ETELTRVKNIVEMDIASNITGVMNKAEMLAIAEMIESADLVNTEINRYLNVDASTMFT
                     IAQQLLTAEKCSTLYYGKD"
     gene            3917..5200
                     /locus_tag="PLB46_09255"
     CDS             3917..5200
                     /locus_tag="PLB46_09255"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRF76745.1"
                     /translation="MINRNSAPGLQLIDRLDINSYKKTSLSNGVDVYYVDSNQEELIR
                     IELRFRAGRWYEPQQAVSRAVCNLMRKGTIQKSAKQISDIVEFYGAHLDVQSGQDYTS
                     MMIFCLSKHLEKIMPVAEEILLEANFPEDEMALFIQKQKQKLRINAQNTDFHANRKFN
                     TVLFGSQHPYGYSVHESTFDAISADQLLQFCRKHYSPGDATIFMAGNITSATLPLLEK
                     HFGAHKGVGKIIAATDKSIHTDSEHKHFIDKDDSVQSSVRIGTLSIPKTHPDFAELNV
                     LNTAFGGYFGSRLMTNIREDKGYTYGIYSSITHLVHASYFSVETEVGNEVCAAAIAEV
                     YKEMDVLKTTPIDDEELTIIKNYMMGGLLRATDGPFNRINVIKNMVMSNLTTEYFDGL
                     AHAIKHVDAERLMELANKYFDYETMKEVICGKKIV"
BASE COUNT         1825 a         1040 c         1028 g         1641 t
ORIGIN      
        1 gaacgtttgt aaattggccg tcaacccata ccaatattgt ttcacccgcc tcagcggact
       61 aaaagcgcgg aggatgggag ttgcggaatg gtaatggtta attcaatgaa tttacaaatc
      121 gcgttttttg gtaatccacc aacccaatcc acaaaatatc acggcatagg ctgcaccaac
      181 tgccaatgat aatgctgtag gcgcaacagg agttggttcc aatcccccgc tcatcgtgtg
      241 gtcaagcgga aatggcacca tttttaaaaa gtaagtcgcc accggcatat aatccccaaa
      301 accatcaggg aaaatacctt cacgtaaaat cacatctatc gggaataaaa aaatcaggaa
      361 tataattact gccaatccgg ttcttttgaa caaggtgcca aaaaaatatc ccattgacat
      421 cataccaagt gattgaataa aatacgcaaa aataaatccg gaatgtccga acatctggtc
      481 gcggtcttcg ggtaaagaat aaatatttcc cgcaactaat ccgctgataa ttaaaataac
      541 agtggtaatt atactcaata agactattaa tgtaattttt ccaataacag cttcctgtct
      601 ggtaagtcca tcgattatat tttggcgtag tgttctataa gtaaactcgt tgcaggttac
      661 aaatattacc aatatggcaa gaataatatt aaagtaacag gcatacaaag tataataatt
      721 ccaaatgttg ggaaacccaa gcacattggc cgatgtaagc ccttcgtcgc cgctgccacc
      781 aaggtttttg cccaccagca atacgagggt catcagcaac aaataaatgg caaataatat
      841 tttaaacgtt ctgtaaggaa ccagttttaa taattctatt ttaagcagac gtatcatatc
      901 gggttattcg gcagttaatt ctaaaaattg ttcttcaagg cgttttttgc gcacattcaa
      961 atgttgcaga taaatatttt tttcgctgag atatttattt aattgagctg cattaaattt
     1021 ttcatctaca ttaattatta tcatactgtt ttcttgtgca gcctttttta ttccggtaaa
     1081 tgattgaatt gcctgcaata ctatttcgtt attatctgca gcaatttcaa cttgtgtttc
     1141 acctttaatt aaatccgcaa cacttccttc ggcaagtaat gttccttttt taataacact
     1201 aacgtgcgta cacacttttt caacttcatc taaaatatgg ctggccaaaa taatggttcg
     1261 gccttgtgca gattgttgcg taataaattc gcgcacctcg gcaatacctt gtgggtcgag
     1321 accattggta ggttcgtcta acaataacac atcgggattt cccaataatg cagaagccaa
     1381 cgccaaacgc tggcgcatac ctaaagagta agtgcgagcg gggctttttt tcctttcaag
     1441 cagatttacc gatgctaaaa cagcatcgat tgttccttca tcaacacctt taattttggc
     1501 gacaactttt aaattttgtt caccactcag gtaagggtaa aaattgggtt gctcaagcag
     1561 cgaaccaatt ctttttctgt tgatatccaa cgagccatta ttaaaccagg aatatgcccc
     1621 tgatgttggc tgaatgatac ctaataacat cccaagtgta gtcgtttttc cgctgccgtt
     1681 cggccccaga attccataaa ctgcgccttt ttcaacacgt aaatcaaggc cgtttacagc
     1741 tttgatgggt ccgtattgtt tttttagtgc ggttgtcgat aaaacagttt ccaaattata
     1801 gtgatttgaa gtaaaagtaa acaattcaac gctatcggca atcaatatgc caaaatctat
     1861 ttgctaaacg gttgtcagta caaaaatgtt acaactacat ttaaattata ttaataacaa
     1921 ttaggaagta aacctgtcct gtatattaaa tcaatatatt tgcatacatg ctgcaagcca
     1981 cttatcagga cgaattaatt gaagccggtt gcgatgaagc cggacgaggt tgtttggccg
     2041 ggccggtagt tgctgctgcg gtaatattgc ccaaaaactt ttttcatccc ctgctcaacg
     2101 attcaaaaca attaaccgaa aacgaacgta ccttgttggc gcctattatt aaagcagccg
     2161 caatagattg gaaaatcggt atttgcgata atcatgaaat tgatgccatt aatattttaa
     2221 aagccagttt caaagccatg cacaaggcgg ttgatcaaat gaaaaaaaag cccggactgc
     2281 ttttaattga cgggaatcgt ttttacaaat atccacgtat accacatatc tgtattgtaa
     2341 aaggagatgc gaagtttatg agtattgcag ctgcttctgt gctggcgaaa acttaccgcg
     2401 atgaattaat gcacaaatta cacaatgagt ttcctattta taactggcaa caaaacaaag
     2461 gatatcccac taaagaccat agaaaagcta ttgctcaatt tggcagcact aactatcata
     2521 gaaaaacatt tcgattactg cctgaaaatg aagttacgct ttttgaataa tcgttttgcc
     2581 tgaataatta ttgtcggtga tttagccaca aatcgaaccg aacttcatct caacatgtta
     2641 atattccact aattacattt actttgcact tatgattgct tttcaaacat tcaaattaga
     2701 taatggactc acggttattg taaacgaaga caataccaca caattatgcg ctgtcaacgt
     2761 attatataaa gttggagcac gtgatgaaat aattactaaa acaggtttcg cgcatttgtt
     2821 tgaacatctt atgtttggcg gctcggcaaa tattcccgat ttcgataccg aattacaatt
     2881 agccggagga acaaacaatg cctatacttc aaacgactta accaattact atgatatttt
     2941 gccggtaagc aatattgaaa ctgctttatg gctggaaagt gaccgtatgc tcaaactcga
     3001 tttttcggaa aaatctttaa acgtgcagcg caatgtggtg tgtgaagaat ttaaagagca
     3061 ctacattaac aaaccctatg gcgatgtttg gcacattttg cgaaaactgg tttacgaaaa
     3121 acatccttat caatggccaa caattggttt ggagttgcaa catgttcagg atgctacttt
     3181 aactgatgta gaaaactttt tttataaata ttatcgcccc aacaatgcca ttttaactat
     3241 ttccggtggt attagtgccg ataaagcttt acaactcgca aaaaaatggt ttggagatat
     3301 tccttcagga gaaacattta atcgcaacat tcccgacgaa ccggagcaac aaactgctaa
     3361 attcctgcat gttaaaaaag atgtgcctgt taatgtgatt tacaaagcat ggaaaatgcc
     3421 cggcagaact gaaccacaat attatgctac caatttatta agcgatgttt tagggttagg
     3481 agaaagttcc cgtttgcaac atgcattaaa aaaagaaaca aaattattta cagatattgc
     3541 tgcatatata acaggtagta ttgataccgg tatgtttatt attaccggca aactagccga
     3601 aggcgttact tatgaacagg cagaaaaagg tatacagcag gaattagata aaatacgaca
     3661 ggctcctgta agtgaaaccg aattaacgag agtaaaaaat attgttgaaa tggatattgc
     3721 cagcaatatt accggtgtga tgaataaagc tgaaatgttg gctattgctg aaatgataga
     3781 atcggcagat ttggtgaata cagaaattaa cagatactta aatgttgatg cctcaaccat
     3841 gtttaccatt gcacaacaat tattaaccgc tgaaaaatgc agcacattat attacggaaa
     3901 agattaattt ataaagatga taaacagaaa cagcgctccc gggttacaat taatcgacag
     3961 actggatatt aatagttata aaaaaacatc attaagtaat ggtgttgatg tatattatgt
     4021 tgacagcaat caggaagaat taattcgtat tgaattgcgt tttcgcgcag gcaggtggta
     4081 tgagcctcaa caggcggtaa gccgcgccgt gtgtaatctg atgcgcaaag gcacgataca
     4141 aaaatcggcg aaacaaattt cggatattgt tgaattttat ggcgcacatc tggacgttca
     4201 aagcggacag gattatactt caatgatgat attttgttta agtaaacatt tagaaaaaat
     4261 aatgcctgta gccgaagaaa tattattgga agcgaatttt cccgaagatg aaatggcatt
     4321 gtttattcaa aagcaaaaac aaaaattgcg tataaatgca caaaacaccg acttccacgc
     4381 aaatagaaaa tttaatactg ttttattcgg cagccagcat ccatatggat acagtgtaca
     4441 tgaatcaaca ttcgatgcaa taagcgccga ccaattatta caattttgtc gcaaacatta
     4501 tagccccggc gatgctacca tatttatggc cggaaatatt acatctgcaa cactgccatt
     4561 gctggaaaaa catttcggtg cacacaaagg tgttggaaaa ataattgcag caaccgataa
     4621 atctattcat accgattctg agcacaaaca ttttatcgac aaagacgatt cggtgcaatc
     4681 aagcgtacgc attggcactt tatctatacc aaaaacgcat cctgattttg ccgaactcaa
     4741 tgtgctgaat actgcatttg gcgggtattt cggctcacgg ttaatgacta atattcgaga
     4801 ggacaaagga tatacttatg gcatatattc ttctatcacg catttagttc acgccagtta
     4861 tttttcggtt gaaactgaag ttggcaatga agtgtgtgct gctgcaattg ctgaagtgta
     4921 taaagaaatg gatgtgttaa aaaccacacc tattgatgat gaagaattaa ccatcattaa
     4981 aaattatatg atgggtggat tattacgtgc aaccgatggt ccgtttaaca gaattaatgt
     5041 aataaaaaat atggtaatga gtaatttaac caccgaatat tttgacggat tagctcatgc
     5101 tattaaacat gttgatgcag agcgattaat ggaattagcc aacaaatatt tcgattatga
     5161 aacaatgaaa gaagtaattt gtggaaaaaa aatagtctga ttgctatttt tttgtttttt
     5221 accagttttc aataagcgtc attttagtta caagcatagc cgaagttgta aaataacttt
     5281 gcgaaatttg cgttagattt actgaacgct gcgatggtta agttattatt gaaatattta
     5341 atgcttgcat tcctgggcat ggggacaatc aacttgtccg cacaatcgac ccttcctgcc
     5401 aatttattat gcgttcaaac agaaataaac ggtgatgtaa cccttacctg gagcgcatct
     5461 gctgaagctt gtggcggatt tcgtgtgttt catcaatatc gctcctacca tgtaccctgc
     5521 aatctccatt tttt
//
LOCUS       DAORKW010000211         5493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_596_length_5493_cov_4.033652, whole genome shotgun sequence.
ACCESSION   DAORKW010000211 DAORKW010000000
VERSION     DAORKW010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_596_length_5493_cov_4.033652"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..741
                     /locus_tag="PLB46_09260"
     CDS             184..741
                     /locus_tag="PLB46_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76746.1"
                     /translation="MEGALAGATGGASLIVTQAVSATARASIDYSKSEGLESWGGFIG
                     DTKAPGDVLKDFISEGAGFAIGKGLENLNISGKVSSEFITKNSSINQVIWAEFVGQMA
                     AAPFEAVYSGIADGLIYSPLAGNPISSLALMLNNKFQPMVYLETVHVKATRTNKGKVK
                     ILNIDLFNKTVEKSAEKNRKKYGYD"
     gene            728..1282
                     /locus_tag="PLB46_09265"
     CDS             728..1282
                     /locus_tag="PLB46_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76747.1"
                     /translation="MAMIKPHKLTIILVIVIIMVLNNSCSNAKSAESTSQWITTYGSV
                     TGISKNLFIEYEVNGRFYKQRFYKGYYGMVGDGEKYELKYNPINASEIEICYSCPIFL
                     PNENTKKMTGKITHVKWIGGGDYKYIIQYCYTVSGKNIVKSQGLPINYKELYPKLAKG
                     QSYYIDVWVDNVYRAVLYLDQPVN"
     gene            1558..2457
                     /locus_tag="PLB46_09270"
     CDS             1558..2457
                     /locus_tag="PLB46_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRF76748.1"
                     /translation="MDPETYGQGNLYDYGYRVYNPRLGKFLSVDPLSSSFPWYTPYQF
                     AGNTPIWAIDVYGLEDQKTNEGTGSTISSSSEPNTVGISETSEESYFSDPTNFVINQK
                     PDEAASKYMQSVKDQLEPAEIVFGGLTLTADAIEKLGGLEKAGKLVDEGKIEVIFNEK
                     LKTWSLDFHGNNTTVPVEFVRDTKSSNLALARQHTKALKCVKAGGTVSNILGIGITGN
                     TVYTEIKDDGMISAGTGLDVAMTAISFIPIGGWITGGGYFAIKLILDGNEMQGEDLLI
                     RKNFLLENTDEIKPIGIQDKLTP"
     gene            complement(2878..3801)
                     /locus_tag="PLB46_09275"
     CDS             complement(2878..3801)
                     /locus_tag="PLB46_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76749.1"
                     /translation="MLTTFELKVLQPIIQPCTANPTEMLAHDLGQYCLDCNKVVHELT
                     HLDNTALINYLTQPGNENICGKIDTAALAPQITLNITKQSNEQRRLYTFGIALLFVFG
                     PLLFSCNDVEHEVIKENIISTYVAPEIEEEAIEEITIDLPNAEDITSQQSVIQELPIT
                     PNTDTAEITLEPVIVAGKTSDKTNGLIVGELELICAVKRVLIKIDTTPLATPEIITSP
                     INRELTLGVYPNPAMSNVTIAYEITEPGSAVLSIFNINGQHITDLLNTNDISTGNFTT
                     AYNVDHLASGMYLVILVNNDKKEVFRMMVGR"
     gene            3974..5467
                     /locus_tag="PLB46_09280"
     CDS             3974..5467
                     /locus_tag="PLB46_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HRF76750.1"
                     /translation="MKKLLLFICCCTVGLVFGQSDITLEDIWVKNRFYAKGIEDIRSM
                     NDGQHYTINEGGAGIGKYTYATGERVGEIFNVTQTHGAVANFRNYMFNDDESAVLLTT
                     QSKPRYRHATYDENYIYDFKSAQTTRLSERGFQMYANFAPAGNRIAYVVDNNLFYKDL
                     TTNEEVQVTYDGKWNYIINGGSDWVYEEELVLIRAFEWSPDGSMIAFYKFDESDVSQF
                     DMTTYKGKLYPQNYSFKYPKVGEVNSKVEVYIYNLNTKTTQKVNINKQYEYIPRIKWT
                     NDSKGLVVFTLNRLQNDLELFMANPESGATQTFFRETAQYYLEINDDLTFLKDNKSFI
                     WKSEKDGNFHLYLYNMSGKLINQITYGNYEVSSLVGVDEKNKKVYYMSSEASIYENHL
                     YSIQLDGKNKQQLTTRFGANSVNFSTGFSYFINTNTSIMQPAYITLCNNKGEVVRVLE
                     DNKTLQDDINNLHIKAPEFFSFTTAEGVSLNGYMIKPTANQNGHSGG"
BASE COUNT         1808 a          817 c         1098 g         1770 t
ORIGIN      
        1 atcagtggat cacattggtc cggaattggt ggtgcagtat gaccgaaata ttcaataatt
       61 ggtggcgctg ttggagctgt aaaagaatat ggaacacaag cagtatccgg aattgttcaa
      121 ggtaaatcat taaaggacgc actctggaat gatgttgatt gggcagatgt tgcaatttca
      181 actgtggaag gtgctttagc tggtgcaact ggaggagctt cgttgattgt tactcaagct
      241 gtctcggcta cagcaagagc ctcgattgat tattctaaaa gtgaaggttt agaatcgtgg
      301 ggtggcttca ttggcgatac aaaagcgcct ggtgatgtac ttaaagattt tatttctgaa
      361 ggtgccggtt ttgctattgg aaagggctta gaaaatttaa atattagcgg aaaggtttca
      421 agtgaattta taactaaaaa ctcgtctata aatcaagtta tttgggctga atttgtaggg
      481 caaatggctg ctgcaccatt tgaagctgta tattctggga tagccgatgg attaatttat
      541 tcccctttag caggtaatcc aatatcaagt ttggccttga tgctaaataa taaattccag
      601 ccaatggttt acctggaaac agtgcatgta aaagctacaa ggactaataa aggcaaagta
      661 aaaattctta atatagattt atttaataaa acagtggaaa aaagtgcgga aaagaataga
      721 aaaaaatatg gctatgatta aaccacacaa gttaacgatt atattagtta tagtcatcat
      781 aatggtatta aataattctt gcagcaatgc taaatcagct gaatctacca gtcaatggat
      841 tactacttac ggctcggtga ctggaatatc aaaaaatcta tttattgaat atgaggttaa
      901 tggaagattc tataagcagc ggttttataa aggctattat ggaatggtgg gcgatggtga
      961 aaaatacgag ctgaaatata accctattaa tgcttctgaa attgaaattt gttattcgtg
     1021 tccgatattt ctacctaatg aaaatactaa aaaaatgaca ggtaaaataa ctcatgtaaa
     1081 atggatcggc ggtggtgatt ataagtatat aatccaatat tgctacacag taagtgggaa
     1141 aaatattgta aagtcccaag gcttaccaat taactacaag gaactttatc ccaaacttgc
     1201 aaaaggacaa agttattata ttgatgtatg ggttgacaac gtttatcgtg cagttttgta
     1261 tttggatcaa ccggtaaatt aatattttga agaattaaat tatgaactta cgtttacact
     1321 aaattgatga gagtagtcat gttaagaact tgagtatttt tttgatttgt ttttatattt
     1381 tcaagaaaaa caattacatt attattttaa acaaaattaa tatatttgct tacacgttct
     1441 ttgaagtgag ccgcataaaa tggtgtattg aaaccctcac aaatcacacc cttttgggat
     1501 gtctttagtg gggcggagtt ggagtttaaa ttatagatat gatttaatgg taaagaaatg
     1561 gatccagaaa cctatggtca aggtaatcta tatgattatg gctatagggt ttataatccg
     1621 agattaggga agtttttaag tgtggatccg ctttctagtt catttccttg gtacactccc
     1681 tatcaatttg caggcaatac accaatttgg gcaatagatg tttacgggtt agaagatcaa
     1741 aaaaccaatg aaggaactgg ttctactata tcttcctcgt ctgaaccaaa tacggtaggc
     1801 atctctgaaa ctagtgaaga atcttatttt agtgatccta ctaactttgt aataaaccaa
     1861 aaacctgatg aagcagcatc aaagtatatg caatctgtta aagatcaatt ggaacctgct
     1921 gaaattgttt ttggaggatt gacacttact gctgatgcta ttgaaaagct tggtggatta
     1981 gaaaaagcag gtaaattggt tgatgagggg aaaattgaag ttatttttaa tgaaaagctt
     2041 aagacatgga gcttggattt tcatggtaat aatactactg ttccagtaga atttgtaaga
     2101 gatacaaaat ccagtaattt agcacttgcc agacaacata caaaggctct aaaatgtgtt
     2161 aaagcaggag gcactgtttc taatatttta ggaattggaa taactgggaa cacggtctat
     2221 actgaaatta aagatgatgg aatgatatct gctggaactg gacttgacgt tgcaatgaca
     2281 gccatatcat ttataccgat aggcggttgg ataacaggtg gtgggtattt cgctattaaa
     2341 ttaattttag acggaaatga gatgcaagga gaagatttat tgataagaaa gaatttttta
     2401 cttgagaata cagatgaaat taagccaatt gggattcaag ataaattaac accatgataa
     2461 gaaatatttt cgattatatc tattatagag tttacagtta ttataaaaat cgttgggagg
     2521 aagcaaatcc agatatatat gccattatgg ctatttctgg attgcccatc ctaaatgcta
     2581 ttgttatcat aaatgttatc ctttcacttt ttaaaataaa tttttcatta aatactgtta
     2641 gtgtaatttt gttgtcctta agcatactag tttatcatct ggtaagtatg cgtaatataa
     2701 aagaaaaatt ttccaaatgg gacaacgaag accagaaatc aaaaactcga aaagggtatt
     2761 tcattatttt gtacattata tgctctgttg ttttattatt tgttgtgcca ttaataattt
     2821 acaatttaaa taattaaaat atgcaggaaa ttatttaaaa gatattatat gtcaagctca
     2881 cctccccacc atcatcctaa acacctcctt tttatcatta ttcaccaaaa taactaaata
     2941 catgcctgaa gccaggtggt caacattata ggcggtggtg aaattgccgg ttgaaatatc
     3001 attggtgttg agtaaatcgg taatgtgttg gccgtttata ttgaagatgg atagaacggc
     3061 acttcccggt tcggtgattt cataagctat ggtaacatta ctcatggctg gattggggta
     3121 aacacctaag gttagttctc ggtttattgg ggaggttatt atttcaggtg ttgctaatgg
     3181 tgttgtatcg atttttatta gtactcgttt aacagcacaa atcaattcga gctcgcctac
     3241 tattagccca tttgttttat cagaggtttt gcctgcaaca ataaccggtt ctaacgtgat
     3301 ttctgcggtg tctgtgttcg gtgttattgg caattcttga attactgatt gttgtgaagt
     3361 aatatcctcg gcgttcggta aatctattgt aatttcctca atcgcttctt cttcaatttc
     3421 aggtgctaca taggtggata taatattttc ttttataact tcatgctcta catcattaca
     3481 ggaaaacaat aaaggtccaa acacaaataa taacgctatg ccgaaggtgt acaatcttct
     3541 ttgctcattt gattgttttg taatattaag tgtgatttgt ggagccaatg ctgcagtatc
     3601 tattttccca caaatatttt cattaccggg ttgcgttaaa taattaatta aggctgtatt
     3661 gtctaaatgc gttaattcat gcacaacttt attacaatcc aaacaatatt gacctaaatc
     3721 atgggctaac atttctgtcg ggttggcagt gcaaggttga ataattggtt gtaatacttt
     3781 caattcaaat gtggtaagca taaatagcgt gtttttggca taattatttc ctttttgatg
     3841 ttgacttgtc accaattggt aaaaatgaat tgtaagcagg ccaaattact ttaagttgac
     3901 attactccgt tcaatttgcg tggttttggc tgcttctgcc tgatttgtta actttgtttc
     3961 aaataatacg agaatgaaaa aattattgct ttttatctgt tgctgcactg tgggtttggt
     4021 gtttggtcaa tctgacatca ccttggaaga catttgggtg aaaaaccggt tttatgccaa
     4081 gggtattgag gacatccgta gcatgaacga tggacagcat tataccatca acgaaggtgg
     4141 agcaggaata ggaaaatata catatgcaac cggtgagcgt gtaggcgaaa tatttaatgt
     4201 cactcaaaca catggagctg ttgctaattt tagaaattat atgtttaatg atgatgagtc
     4261 ggcagtattg ctcaccacac aatccaaacc gcgttatcgc catgcaactt atgatgaaaa
     4321 ttatatttac gatttcaaat cggcgcaaac aacacggctg tcagaacgag gatttcaaat
     4381 gtatgcgaat tttgcacctg ctggcaaccg tattgcttac gtagtggata ataacttgtt
     4441 ttataaggat ttaactacca atgaggaagt tcaagttact tatgatggca aatggaacta
     4501 tatcattaac ggcggcagcg attgggtgta tgaagaagaa ttggttttaa tccgagcctt
     4561 cgaatggagc cccgatggca gcatgattgc attttataaa tttgatgaat cggatgtttc
     4621 gcagtttgat atgactacct ataaaggcaa actgtatccg caaaattatt catttaaata
     4681 tccaaaagta ggggaggtga acagtaaagt ggaggtttac atctacaatt taaatacaaa
     4741 aactacgcag aaagtaaata ttaataaaca atacgaatat ataccacgaa ttaaatggac
     4801 taacgattca aaaggcctgg tggtgtttac tttaaacaga cttcaaaacg atttggaatt
     4861 gtttatggcg aatcctgaat caggtgctac acaaacattt ttccgcgaaa cagctcaata
     4921 ttatctcgaa ataaatgatg acctcacctt tttaaaagat aacaaatcat ttatttggaa
     4981 aagcgaaaaa gatggtaatt ttcatttgta tttatacaat atgtcgggca aactgattaa
     5041 tcaaattact tacggcaatt atgaagtaag cagtttagtt ggtgtggatg aaaaaaataa
     5101 gaaggtatat tacatgagtt cagaagcatc gatttacgaa aatcatttgt attcaataca
     5161 gttggatgga aaaaacaaac aacagcttac tactcgtttt ggtgccaact cagtaaactt
     5221 tagcaccgga ttttcttatt tcatcaatac gaataccagt attatgcagc ctgcttatat
     5281 tacgttatgt aataataaag gtgaggttgt tcgggtgttg gaggataata aaacattaca
     5341 agacgatatt aataacctac acattaaagc acctgagttt tttagtttta caactgcaga
     5401 aggtgtatcg ttaaatgggt atatgattaa accaaccgca aatcaaaacg ggcattcggg
     5461 gggctgaaat tgccgcggaa ttgggcgatt tac
//
LOCUS       DAORKW010000212         5481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_598_length_5481_cov_5.454478, whole genome shotgun sequence.
ACCESSION   DAORKW010000212 DAORKW010000000
VERSION     DAORKW010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_598_length_5481_cov_5.454478"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1597
                     /locus_tag="PLB46_09285"
     CDS             65..1597
                     /locus_tag="PLB46_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRF76751.1"
                     /translation="MYRGSPASTGFGSFVKGTNSGGNVIWTLSEPYGASDWWPCKQTL
                     NDKIDSIDVFVTSPELYKVGGPGLLIDAVTTGDNITWHWKSNYPIVTYLIGIVVSVYD
                     VYEFYAPHKDDSVLFYNLIYPETFFDAVAGIDAIVPSFELFTDIYGNYPFAAEKYGHM
                     QFGWGGGMEHQTMSSVTHFGYELLVHEMAHQWFGDKITCASWRDIWLNEGFATYSTWL
                     SYDFSDDPNNYYEGWLNGTRSVVVSQPGGSVWVDDTTLVSRIFDGRLSYYKGGWLLHM
                     LRKQIGEDEFFEGIKNYISDADLSYGFVTTNDLIQHLETAADTSLTEFFDDWFYGQGF
                     PTYHVLWTQDDNGFVTIALSQSTSHPSVDFFEMDVPFRIKGENDSIDIHLKHTQNGQL
                     FSIPIPFEANAVILDPAVELLHGESIVTKTELLENTVQLVVYPTLVQDKINIQLFALT
                     EDEYEIMIYSGSADLVMQSKFVADSQTAKFDIDVTTWPSGLYVIVVKNGSTDIVQKVI
                     IL"
     gene            1628..2797
                     /gene="purT"
                     /locus_tag="PLB46_09290"
     CDS             1628..2797
                     /gene="purT"
                     /locus_tag="PLB46_09290"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HRF76752.1"
                     /translation="MKKIMLLGSGELGKEFVIACKRLGQYVIAVDSYNDAPAQQVADE
                     REIISMLDGDALDAIVKKHQPDIIVPEIEAIRTERFYDYEKWGIQVVPSARAANFTMN
                     RKAIRDLAAKELGLRTAKYAYANSFEELAAAVHEIGMPCVVKPLMSSSGKGQSVIKSE
                     DDIIKSWEYAQSGKRGDIAEVIVEAFVRFDSEITLLTVTQKNGPTLFCPPIGHRQERG
                     DYQESWQPAYIKPEHLAEAQNIADKVTAALTGAGIWGVEFFLAPEGVYFSELSPRPHD
                     TGMVTLAGTQNLNEFELHARAVLGLPIPGIELLKCGASAVILANEESNDFHFEGIQNA
                     IVEPKTDIKIFGKPTTRKYRRMGVTLAYDEPHADVQQVKQKAINNAEKVRIINGK"
     gene            complement(2847..4196)
                     /gene="accC"
                     /locus_tag="PLB46_09295"
     CDS             complement(2847..4196)
                     /gene="accC"
                     /locus_tag="PLB46_09295"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRF76753.1"
                     /translation="MFKKILIANRGEIALRIIRTAKEMGIKTVAVYSTADKDSLPVRF
                     ADEAVCIGPPSSKESYLSIPKIMAAAEITNADAIHPGYGFLSENDMFSEICAKYGIKF
                     IGPTPDQIRKMGDKITAKDTMKKAGVPVVPGSDGLLKDVADAKKQAKKIGYPVIVKAT
                     AGGGGKGMRIVWQESEMESAFSTASMEAKASFSNDGLYMEKFIEEPRHIEIQVAGDRY
                     GKACHLSERDCSIQRRHQKLVEESPSPFLDDKLRRKMGDAAVKAAESIAYEGVGTIEF
                     LTDKHRNFYFMEMNTRIQVEHPVTEEVVDFDLIKEQIKIAMGEKISGKNYFPELHAME
                     CRINAEDPRKNFSPSPGKITSFHSPKGHGVRVDTAVYAGYTIPQYYDSMIAKLICKAR
                     TREEVILKMQRALDEFIVEGIKTTIPFHKKLMKDERFRAGVFDTSFMNSFDLSDLPE"
     gene            complement(4266..4760)
                     /gene="accB"
                     /locus_tag="PLB46_09300"
     CDS             complement(4266..4760)
                     /gene="accB"
                     /locus_tag="PLB46_09300"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRF76754.1"
                     /translation="MDFKEIQELIRMVNKNNIAELTIENQDFRIKIKTALAATYTTQA
                     PVVMQQMPQQVAMPQIQQAATSEKPQTTQESAKSEEASGGNTITIKSPIVGTFYRSPG
                     PDKEVFVKVGDEIGVGTKLCIIEAMKLFNEIESEHSGRIVKILVDDASPVEYDQPLFL
                     IEPK"
     gene            complement(4768..5331)
                     /gene="efp"
                     /locus_tag="PLB46_09305"
     CDS             complement(4768..5331)
                     /gene="efp"
                     /locus_tag="PLB46_09305"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRF76755.1"
                     /translation="MATTADIRKGLCIELNNDVWQIVDFQHVKPGKGNAFVRTKIKSL
                     TTARTLENTFPSGADLKIVRVERRTFQYLYNDETGYTFMNNETFDQVTLDAEMVEGKE
                     FYKEGQEIDILFHADTEKPLFCELPAHIILAVTYAEPGVKGNTATNAYKNATVETGAN
                     IQVPLFVEEGDVIKIDTRSFEYVERVK"
BASE COUNT         1638 a          966 c         1109 g         1768 t
ORIGIN      
        1 caaggcgggt gaaatattat ttggtgaaga gttatctgca gaggaaaatt gaagcacaac
       61 tgtcttgtac cgcggaagcc ctgcttcaac tggatttgga tcatttgtga aaggcaccaa
      121 ctcgggtgga aatgtaattt ggactttatc ggagccttat ggtgcaagcg actggtggcc
      181 atgtaaacaa acactcaacg ataaaattga ttctattgat gtatttgtta cttcacctga
      241 attatacaaa gtaggcggac cgggattatt aattgatgct gtgacaaccg gtgataatat
      301 tacatggcat tggaaaagta attatccaat tgtcacatat ttaattggta ttgtagtgag
      361 tgtttatgat gtatatgaat tttatgctcc tcataaagac gactctgtgt tgttttacaa
      421 cctgatttat cctgaaactt tttttgatgc agttgcagga attgatgcta ttgtaccttc
      481 atttgaattg ttcaccgaca tttacggaaa ttatcctttt gcagcagaaa aatacgggca
      541 tatgcaattt ggatggggtg ggggaatgga acatcaaacc atgagttctg tcacacattt
      601 tggatatgaa ttattggttc atgaaatggc gcatcaatgg tttggcgata aaattacttg
      661 cgccagttgg cgagatatat ggttgaacga agggtttgca acttatagca catggttgag
      721 ttacgatttt tctgatgacc caaataatta ttatgaaggt tggttaaatg gaacccgaag
      781 cgtggttgtg tcgcaaccgg gaggttctgt gtgggtggat gatactacat tggtcagccg
      841 catttttgat ggcagattat cctattataa aggcggatgg ttgttacata tgttgcgtaa
      901 acaaattggg gaagatgaat tttttgaagg gataaaaaat tatatttcgg acgctgattt
      961 gagttatgga tttgttacaa caaacgattt aattcaacat cttgaaactg ctgcagatac
     1021 ttctttaact gaattttttg atgattggtt ttatggacaa gggtttccaa cctatcatgt
     1081 attgtggacg caggatgata atggttttgt caccattgca ttaagtcagt caacgtccca
     1141 cccaagtgtc gacttttttg aaatggatgt gccttttaga atcaaaggtg aaaatgattc
     1201 tatcgatatc catttaaaac atacacaaaa cggacaattg ttttctattc caattccgtt
     1261 tgaagcaaac gcagttattt tagaccctgc tgtcgaattg ctgcatggtg agtcgattgt
     1321 taccaaaact gaattgttag aaaatactgt tcagttagtt gtgtatccaa cattagtaca
     1381 agataaaatt aatattcagc tatttgcttt aactgaagat gaatatgaaa ttatgattta
     1441 ttcaggctcg gcagatttgg taatgcaatc gaaatttgtt gccgattcgc aaactgcgaa
     1501 atttgatatt gatgttacaa catggccttc cggactatac gtgattgtgg taaaaaacgg
     1561 cagcacagac atcgtgcaga aagtgataat tttgtaaata tttttaaata aaaaataatt
     1621 gttattgatg aaaaaaataa tgttattagg cagtggtgag ttagggaaag aatttgttat
     1681 tgcctgtaaa cgattgggtc aatatgtaat tgcggtagat agttataatg atgctcctgc
     1741 tcagcaagtt gcagatgaaa gagaaattat ttcgatgttg gatggagatg cattggatgc
     1801 aattgtaaaa aaacatcaac ccgatattat tgttccggaa atagaagcta tacgcaccga
     1861 acgattttac gattatgaga aatggggcat tcaggttgtg ccaagtgcac gtgccgctaa
     1921 tttcactatg aatagaaaag ctattcgcga cctagctgca aaggagctgg gtttaagaac
     1981 tgcaaaatat gcttatgcta atagttttga agaattggct gctgccgtgc atgaaattgg
     2041 aatgccatgc gtagtaaaac ctttgatgag ttccagtgga aaggggcaat cggtaattaa
     2101 atcagaggac gatattatta aatcatggga atatgcacaa agcggaaaac gaggcgacat
     2161 tgctgaagta attgttgaag catttgttcg ttttgattcg gaaattactt tactaactgt
     2221 tacgcaaaaa aatggaccaa cgttattttg tccaccaata gggcacagac aagagagagg
     2281 cgattatcag gaaagctggc aacctgcata tattaaaccg gaacatttag cagaagcaca
     2341 aaatatagca gataaagtaa cagcagcatt aacaggggca ggaatttggg gtgttgaatt
     2401 ttttcttgca cctgagggtg tttatttttc ggaattaagt cctcgaccac atgataccgg
     2461 aatggtaaca ttagcaggca ctcaaaatct caatgaattt gaattgcatg cacgtgcagt
     2521 tctgggttta ccaattcctg gaattgaatt attaaaatgt ggagcaagcg cagttatttt
     2581 agccaacgaa gaaagcaatg atttccattt tgagggaata caaaatgcta ttgttgaacc
     2641 taaaactgat attaaaatat tcggcaaacc cacaaccaga aaatatcgca gaatgggtgt
     2701 aacattagct tacgatgaac cccatgctga tgtacaacag gtgaaacaaa aagccattaa
     2761 taatgcggaa aaagttcgca taataaacgg taaataaaaa aagacaagct ttgcggcttg
     2821 tctttctatt tagtttaatg attgtattat tccggtaaat cagataaatc gaaactattc
     2881 ataaatgatg tatcaaaaac acctgctctg aatctttcgt ctttcattaa ttttttgtgg
     2941 aatggaattg ttgttttaat tccttcaaca ataaactcat ctaaagcacg ttgcattttt
     3001 aaaatcactt cttcacgtgt tcttgctttg caaattaact tcgcaatcat actatcataa
     3061 tattgtggaa tagtatatcc tgcataaaca gcagtgtcaa cgcgcacacc gtgaccttta
     3121 ggtgaatgga agctggtaat ttttccggga cttggagaga aattttttct tgggtcctca
     3181 gcattaatac gacattccat ggcgtgtaat tcaggaaaat aatttttacc cgaaattttt
     3241 tcgcccattg cgattttaat ttgttcttta atcaaatcaa aatcaacaac ttcttctgta
     3301 acaggatgtt caacctgaat acgggtattc atttccatga aatagaagtt gcggtgttta
     3361 tcggtgagga attcaattgt tccaacacct tcatatgcga tagattcagc agctttaacg
     3421 gcagcatcac ccattttgcg gcgtaattta tcatctaaaa acggagaagg actttcttct
     3481 accaatttct gatggcgacg ttgaatagag cagtcgcgtt cgcttaagtg acacgctttt
     3541 ccatagcggt cgcctgcaac ctggatttca atatggcgag gttcttcaat aaatttctcc
     3601 atatataatc catcattact aaacgacgct tttgcttcca tgcttgcagt tgaaaatgcg
     3661 ctttccattt cagattcctg ccatacaata cgcattcctt ttccaccacc accggcagtt
     3721 gcttttacaa ttaccgggta gccaattttt ttagcttgtt tttttgcgtc tgcaacatct
     3781 tttaataaac catcgctacc aggaacaaca ggcacaccgg cttttttcat ggtgtcttta
     3841 gcggtgattt tatcgcccat tttgcgaatc tgatctggcg ttggtccgat aaatttaata
     3901 ccgtatttcg cacaaatttc tgagaacata tcgttctcac ttaaaaatcc atatcccggg
     3961 tgtatagcat cagcatttgt aatttcagct gcggccataa ttttaggaat acttaaatat
     4021 gattctttag aagatggagg tccgatacat acggcttcat ctgcaaaacg aacaggtaaa
     4081 ctgtctttgt cagcagtgga gtatacagca acggttttta tacccatttc tttagctgta
     4141 cgaataatgc gaagtgcaat ttcaccacgg ttggctatga gtatcttttt aaacatttgt
     4201 aagtttatgg attaactaaa tagctgaaaa ataaatagct cccaaagtaa gggagctaat
     4261 gctaattatt taggttcaat aagaaacaat ggctggtcgt attctacggg actagcatca
     4321 tcaactaaaa ttttaacgat acgacctgag tgttcgcttt caatttcgtt gaataatttc
     4381 attgcttcga tgatacataa tttagtacca actccgattt catctccaac tttcacaaat
     4441 acctctttat ccggtcccgg ggaacgataa aaagtaccta caattggcga cttaatagta
     4501 atggtatttc caccggatgc ttcttcagat ttagcagatt cctgagtggt ttgaggtttt
     4561 tcagatgttg ctgcctgctg aatttgcggc atggcaacct gctgaggcat ttgttgcatt
     4621 actaccggcg cttgcgtggt gtaggtagcg gctaatgccg ttttaatttt tatacgaaaa
     4681 tcctgatttt cgatagtaag ttcagcgatg ttgtttttgt tcaccatcct gattaactcc
     4741 tgaatttctt taaagtccat agtaaggtta ttttacacgt tcaacatatt caaatgatcg
     4801 ggtgtcaatt ttaataacat ccccttcttc tacaaacaag ggcacttgga tattggcacc
     4861 tgtttcaacc gttgcgtttt tataggcatt agttgcggta tttcctttta cccccggttc
     4921 tgcataagta actgctaaga taatatgtgc aggcaattcg caaaagagtg gtttttcggt
     4981 atcagcgtgg aaaagaatgt caatttcctg tccttcctta taaaattctt taccttctac
     5041 catttcggca tccagtgtta cttggtcgaa agtttcatta ttcataaatg tataaccggt
     5101 ttcgtcgtta tacaagtatt gaaaagtgcg acgttcaacg cgtacaattt ttaaatctgc
     5161 gcctgatggg aaagtgtttt ccaaagtgcg ggcagttgtt aaacttttaa tttttgtgcg
     5221 gacaaaagcg ttgcctttac ccggttttac gtgttgaaaa tctacaattt gccaaacatc
     5281 gttattaagt tcgatgcaaa ggccttttct aatatctgca gtagttgcca taactgttaa
     5341 ttagtgatta tttgcgaatc ggcaatcatt gtattgcctt gttatttatc agcttactga
     5401 actcaataag cccatttaat taatgttgcg ccccaggtaa atcctccacc aaaagcagcc
     5461 aacacaacgt tgtcgccttt t
//
LOCUS       DAORKW010000213         5463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_600_length_5463_cov_4.600777, whole genome shotgun sequence.
ACCESSION   DAORKW010000213 DAORKW010000000
VERSION     DAORKW010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_600_length_5463_cov_4.600777"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="PLB46_09310"
     CDS             <1..1069
                     /locus_tag="PLB46_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76756.1"
                     /translation="GLHTGSVTFSITTDAPEYSPFVIPEVVLPIIDNDSMPGINFVIP
                     AILALTEGEGEVAVAIALKSVPTDTVYIQVIPSDQLRITGAPGEAVTLVFAPNTSAMS
                     AHNAVVKAYDDLLYEGDHVGTVEFVITTIDADYSLFSLAPFDVSITDNDLIPGITFED
                     TTGLIGVENMIDTLHFSVYLNSIPTSTVTINFDPDINLDLGKGKDADVSMKFKEDSSL
                     IARVMNVMIFNDPIVEGPHIGTIVCSITTTDTFYADFTIPNIYVAITDDDGTAIMNYD
                     PDIFQVFPTVTTNTLQYKVDENFANATIRIFSMDGRMTAEHNLVGKTGTRDVQHLNSG
                     TYIIMVSNNEAMFYQRFQVIR"
     gene            1144..3567
                     /locus_tag="PLB46_09315"
     CDS             1144..3567
                     /locus_tag="PLB46_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HRF76757.1"
                     /translation="MPKFAPVANPKQQSTDSKTTCFHCGEVCNDHEFHLEDKYFCCFG
                     CKTVYEILRDNDLCSYYDMQEHPGITQKNKVAANKYSVLDKQSVREKFIRFADGNQTH
                     VVFYLPTMHCSSCIWLLEHMHQMNPAVLQSRVDFPKKEITLVFDESKISLGEVAAMLD
                     SIGYEPLLSLQDIEKKPEKQLQKSRLIKIGISGFAFGNIMMMAFPEYLAGGAVEDVSL
                     QILFRYLSLVLALPVFFYCAGEFYTSSWKSIKNKFLNIDVPIALAIIFTFTRSLWAVF
                     VEHEGGYFDSMTGIVFFMLVGRYFQDLTYKTISFDRDYKSYFPLSVALVNDGAEQQIP
                     LSEIKEGDVIVVRNHELIPADALLILGKGNIDYSFVTGEADSVDKKIGELIYAGGKQV
                     GAAIQLKVIKPVAQSYLTQLWNNINASHKEDERKDFVQTLGRYFALIVLVLSAIASLF
                     WIVQGDMERAADAFITPMIVACPCALLLSATFTNGNAIRYFGNFGIYLKNANVIAAFN
                     KITTIVFDKTGTLTQSNTTGTSYNGDVLSNEERICVAALAAQSNHPYSRAIANMLGKS
                     TGAEITDFKEFPGKGISAKINGNELSLGAAEFVGVENKTVQHGITYVAINGKVKGHFN
                     FANRYREGFDNMVNNLKGNYELYLLSGDNDSDRTYLSSYFGANKLYFNQTPHDKLAFI
                     KQLQAQNKHVMMLGDGLNDAGALQQSDIGIAVSQNSNFTPASDVVMDVNQLSKLEQLF
                     KFAKASKLVITGSFVLSIIYNVIGLYFALQGMLAPVIAAILMPLSTITIVSFTTGMTT
                     IAAKRLLKK"
     gene            3809..3991
                     /gene="ccoS"
                     /locus_tag="PLB46_09320"
     CDS             3809..3991
                     /gene="ccoS"
                     /locus_tag="PLB46_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HRF76758.1"
                     /translation="MSVIILLIGASILVAGGFLVAFIWSVKHGQYEDDYTPGVRMLFD
                     DQKASNHPTTSTKTKK"
     gene            3995..>5463
                     /gene="ccoN"
                     /locus_tag="PLB46_09325"
     CDS             3995..>5463
                     /gene="ccoN"
                     /locus_tag="PLB46_09325"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRF76759.1"
                     /translation="MDYVKVSYDNKIVKQFALATVFWGIIGMTVGLWIALELVFPQLN
                     MGVPQLSFGRDRPIHTNAVIFAFVGNGIFMGVYYSLQRLCKAPMFSKFLSNLHFWSWQ
                     LIIVLAALSLSMGITTGKEYAELEWPIDILITLVWVVFGINMMGTIIKRRERHIYVGI
                     WFYIATFVTVAMLHIVNSLELPVSLFKSYSIYAGVQDALVQWWYGHNAVAFFLTTPYL
                     GLMYYFLPKAANRPVYSYKLSIVHFWTLIFLYIWAGPHHLLYTALPHWAQTLGTVFSV
                     MLIAPSWGGMVNGLLTLRGAWDKVREDVVLKFMVVAVTAYGMSTFEGPMLSLKNVNAI
                     GHFTDWIVAHVHIGALGWNGFLTFGILYWLIPKMWRTTLYSKSAANFHFWIGTLGILV
                     YAVPMYWGGFTQALMWKDFNAEGQLSYTFLETSTQLKGMYALRSIGGTLYLIGVFLMA
                     WNLWKTAAQGSFLAEEEGEVPEMPRTYNKTKGESWHRPL"
BASE COUNT         1675 a          908 c         1112 g         1768 t
ORIGIN      
        1 aggattgcat accggctcag ttacatttag tataacaact gatgcgcctg aatattcacc
       61 atttgttatt cccgaagttg tgcttcccat tatcgacaac gatagtatgc cgggaattaa
      121 ttttgtaata cctgcaattt tagcgttaac cgaaggagaa ggtgaagttg ctgttgctat
      181 tgctttaaaa tcagtaccaa cagacactgt gtatattcag gtgataccca gcgatcaatt
      241 gcgtattacc ggagcacccg gtgaagctgt tactttagtg tttgcgccaa atacttctgc
      301 catgagtgct cacaatgcag tggtaaaggc ctatgatgat ttgttatatg aaggtgatca
      361 tgttggaaca gtggaatttg taattactac tattgatgct gactattcct tgttttcact
      421 tgccccgttt gatgtatcaa ttacagacaa tgacttaatt ccgggaatta cttttgaaga
      481 taccacggga ttaattggag tagaaaatat gattgatacc ttacattttt ccgtgtattt
      541 aaatagtatc cccacttcaa cagttaccat caattttgac cctgatatta atttggattt
      601 aggaaagggt aaagatgcag atgtaagtat gaaatttaag gaagacagct cactcattgc
      661 acgtgtaatg aacgttatga tttttaatga cccaattgtt gaaggtccgc atataggaac
      721 catagtttgc agtatcacaa ccactgatac cttttatgca gattttacta ttcccaacat
      781 ttatgtggca attaccgatg atgatggcac tgcaataatg aattatgacc cggatatttt
      841 tcaggtattt ccaactgtta caaccaatac actccaatat aaggtggatg aaaattttgc
      901 caatgccaca atccgcatat tttcgatgga tggccgtatg acagcagaac acaaccttgt
      961 cggaaaaacc ggaacacgtg atgtacagca ccttaatagt ggtacctata taataatggt
     1021 gtcgaacaat gaagcaatgt tttatcagcg ttttcaggta atccgataaa tatcggaaaa
     1081 tggtgaccta agtcatttta tgtaaggtgc aataggagat agctttgaca ttatttttca
     1141 gtaatgccaa agttcgcacc tgtagcaaat cccaaacaac aatctacgga tagtaaaact
     1201 acctgctttc actgtggtga agtatgtaat gaccatgaat ttcatttaga agataaatat
     1261 ttttgttgct tcggatgtaa aaccgtgtat gaaattttgc gcgacaacga tttgtgttct
     1321 tattacgaca tgcaggagca tccgggtatt acacaaaaaa ataaagttgc agcaaataaa
     1381 tattccgttt tagataaaca aagtgtacgc gaaaaattta ttcgcttcgc ggatggcaat
     1441 caaacacatg ttgtatttta tttgcccacc atgcactgta gttcttgtat ttggttgctc
     1501 gaacacatgc atcaaatgaa tcctgccgta ttacaatcgc gggttgattt tcctaaaaaa
     1561 gaaattacac ttgtttttga tgagtcgaaa attagtttag gtgaagtggc tgcaatgctt
     1621 gattccattg gatatgagcc gttgttaagt ttgcaggata ttgaaaaaaa accggaaaaa
     1681 caattacaaa aatcaaggct gattaaaatt ggtatttcag gatttgcttt tggtaatatt
     1741 atgatgatgg cgtttccgga atatttagcc ggcggcgctg ttgaggatgt ttcactgcaa
     1801 attttatttc gctatttatc attagtatta gcattaccgg tattttttta ttgtgcaggc
     1861 gaattttata cttcctcctg gaagagtatt aaaaataaat tcttaaatat tgatgtccct
     1921 attgcattag ccatcatttt tacatttacc agaagtttat gggccgtttt tgtggaacac
     1981 gaaggtggtt attttgattc tatgaccggc attgtatttt ttatgttggt gggtagatat
     2041 tttcaggatt taacgtataa aactatctct ttcgacagag attataaatc gtattttcca
     2101 ttatcagtgg ctttagtgaa tgatggtgct gaacaacaaa ttccattatc cgaaattaaa
     2161 gaaggtgatg ttattgtagt gcgcaatcac gaattaattc ctgccgatgc attacttatt
     2221 ttgggcaaag gaaatattga ttacagcttt gtaacaggtg aagcagactc ggtggacaaa
     2281 aaaatcggag aactgattta tgccggtgga aagcaggtag gggctgccat tcagcttaaa
     2341 gtaattaaac cggttgcaca aagttacctc acgcaattat ggaataatat taatgccagc
     2401 cacaaagaag acgaacgaaa agattttgta caaacgttgg gaagatattt cgcattaatt
     2461 gtactggtat tatctgcaat tgcatcttta ttttggattg tgcagggtga tatggaacgt
     2521 gcagcggatg catttattac accaatgata gtggcttgtc cttgtgcact tttattatct
     2581 gcaacattca cgaatggaaa tgccattcgg tatttcggaa attttggtat ttatttaaaa
     2641 aatgccaatg tgattgctgc gtttaataaa attactacca ttgtatttga taaaacagga
     2701 acattaacac aatcaaatac aacaggtact tcctacaatg gtgatgtatt atcaaacgaa
     2761 gaaagaattt gtgtagccgc acttgcagct caatctaatc acccatatag tcgcgctatt
     2821 gcgaatatgc taggcaaaag cactggtgct gaaataaccg attttaaaga gtttccgggc
     2881 aaaggcatca gcgcaaaaat aaatggcaac gagttgagtt taggtgcagc tgaatttgtt
     2941 ggtgtggaaa ataaaacggt acaacatggt attacctacg ttgcgataaa cggcaaagta
     3001 aaagggcatt ttaattttgc taatcgctat cgggaagggt ttgataatat ggtaaataat
     3061 ttaaaaggta attatgaatt gtatttatta agtggcgata atgatagtga taggacatat
     3121 ctaagcagtt attttggtgc taataaactt tactttaatc aaaccccaca tgataagttg
     3181 gcatttatca aacaattgca agcgcaaaat aaacatgtga tgatgttagg cgatggcctt
     3241 aacgatgcag gtgcattgca acaaagtgat attggtattg ccgttagtca gaacagcaat
     3301 tttacgcctg caagcgatgt ggtaatggat gtaaatcaat tgagtaaact ggaacaactg
     3361 ttcaaatttg caaaagcatc caaactggtt ataaccggaa gttttgtatt gtccatcatt
     3421 tataatgtaa ttggattata ttttgctttg caaggcatgt tagcgcctgt cattgcagct
     3481 attttaatgc cattaagcac catcactatt gtgagtttta caaccggcat gactaccatt
     3541 gctgcaaaaa ggttgcttaa aaaataagtt gttgtttata gatgaaaaaa ataaattgtt
     3601 gaacatccca ttcaacaaaa atgcaattac aggaataatt gctgaaaggc cttccaatag
     3661 taactttcca gcttagtgtt tcaaccttac ctagtatcag aaattatttt tttcaagtgt
     3721 caactttaat atggtgacat aaatcaccat taggtgtgaa gtaagtcatc tgtggccaat
     3781 atatactgtg tcaacttagc atctgattat gagcgtaata attctgttaa ttggagcaag
     3841 cattttggta gccggaggat ttttggttgc cttcatttgg tcagtaaaac acggacaata
     3901 tgaagacgat tacacaccag gtgtcagaat gttatttgat gaccagaaag caagtaatca
     3961 cccaacaaca tctactaaaa ccaaaaagta acgtatggat tacgtaaaag tcagttacga
     4021 caacaaaatt gtaaaacagt ttgcattggc tactgttttc tggggcatta ttggaatgac
     4081 cgtaggtttg tggatagcac ttgaattggt gtttccgcaa cttaacatgg gtgttcctca
     4141 attatcattt ggccgcgaca gaccaattca cacgaatgcg gtgatttttg catttgtggg
     4201 taatggtatt ttcatgggcg tgtattattc gctgcaacgt ttgtgtaaag caccgatgtt
     4261 tagcaagttt ttaagtaacc tccatttctg gtcatggcag ctaattattg tattagcggc
     4321 gctttcgctt tcaatgggta ttacaaccgg taaagaatac gctgaattag aatggccaat
     4381 cgacatttta attacattgg tatgggttgt tttcggtatt aatatgatgg gtaccatcat
     4441 taaaagaaga gaacgccata tttatgtagg tatttggttt tacattgcca catttgttac
     4501 agttgccatg ttgcacattg taaactcatt agaattacca gtttcattat ttaaaagtta
     4561 ttccatttat gccggtgtgc aagatgcatt ggtgcagtgg tggtatggtc acaatgcggt
     4621 tgcattcttc cttactacac catatttagg tttgatgtat tatttcttac ctaaagcagc
     4681 aaaccgtccg gtatattctt ataaattatc tattgtccat ttctggacac tcatattctt
     4741 atacatttgg gcaggtccac accatttatt atatactgca ttaccacact gggctcaaac
     4801 tttgggtact gtgttttcag taatgttaat tgctccatca tggggtggta tggtgaacgg
     4861 attattaacc cttcgtggtg catgggataa agtgcgtgaa gatgtagtat taaaattcat
     4921 ggttgttgct gtaaccgctt acggtatgtc aacttttgaa ggcccaatgt tatcgttgaa
     4981 gaatgtgaac gcaattggtc actttacaga ctggattgta gctcacgtgc acattggtgc
     5041 tttaggttgg aatggtttcc tcacatttgg tattttgtat tggttaatcc ctaaaatgtg
     5101 gagaacaacc ttatactcca aatctgctgc aaacttccat ttctggattg gcacattggg
     5161 tattttagtg tatgcagtac caatgtattg gggtggtttc acacaagcat taatgtggaa
     5221 agattttaat gctgaaggtc agttatctta tactttcctt gaaaccagta cccaattaaa
     5281 aggtatgtat gcattgcgtt caataggtgg taccttatat ttaattggtg tattcttaat
     5341 ggcatggaac ttatggaaaa cagcagcaca aggatcattc cttgcggaag aagaaggtga
     5401 agtaccagaa atgccaagaa cttataataa aacaaaaggt gaatcctggc atcgcccact
     5461 tga
//
LOCUS       DAORKW010000214         5453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_601_length_5453_cov_5.628566, whole genome shotgun sequence.
ACCESSION   DAORKW010000214 DAORKW010000000
VERSION     DAORKW010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_601_length_5453_cov_5.628566"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="PLB46_09330"
     CDS             <1..1701
                     /locus_tag="PLB46_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76760.1"
                     /translation="AKQAWYDLSLAVDPNNANVVAMGGLHLWRSRNAGETWERMTTGG
                     LDSVLIRFMHVDQHEITFRNSDEVYFGNDGGIYRTTNFTAEQPFIYDRNYGYNVTQFY
                     SVAVHPTEGVSQLMGGTQDNGTPYTYDSGNAPFKMVSGGDGAFTAYDHEKPEVFYTAS
                     QLKRLFRFNNGGFEMPDTITNPNLEDYNTLFINPFDIDASDPEVIYQCSNIGLMRLKN
                     ASTADTGQWLKCVNLNGVLSAIASSPAAPGSLFIGKSSGSGDIYRLDDSYNSDATVVP
                     FNLDPFDSLPDAGFLSTVYCSSIVVDVNDANHVIVTYSNYNVKSVWESFNAMAFEPTW
                     HCIEGNLQDMPVYWSAIHPDFPNIVYLATELGVFYTNNVNGYSTNWMPCTNFPIVRTD
                     MLRIKSAEQLLVAATHGRGLWQAQLSATGTLNNLIWQERGPNNFGGRTRTIMIDPNDP
                     SGKTVWAGSVGGGLWKTTNIDAVPIIQPEIVESISLQVYPNPAADYINLHVTFAQPSN
                     STISLLDMQGNIIAEIPSAKGTAQQIIKWNIPANLGAGNYFIMVRSGQQKMVKKIIIL
                     "
     gene            1712..3130
                     /locus_tag="PLB46_09335"
     CDS             1712..3130
                     /locus_tag="PLB46_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76761.1"
                     /translation="MKKLICIVLIIASSSIFAQDWMPFIPNANHFISHESVYNLNQDS
                     TVIEGDMTQYFFNRHFPDPEMESCFDSIDDIEEEYFVIDRCDVITDTFYFYSAYTPGF
                     KVKFINPQTIGYSWDVPNTNPVSDWSFVTISYDSIGTETVFDVVDSVKYFSINVDVEL
                     PDSDAIDKVVIKLSKSNGFIQYLNWFRFENPRLLYPYYTELLEGYSVDGINKGFVIPE
                     WDDYFHYVEDDVLIWKATYPNGSIRYFTDTVLNIYKDDELVELITTNDVFTFYKEAYQ
                     YVLGGPLCNVMYSPASLNDESFENSGMYSLGFVINHIDKSGLNPQISKQIYTDRIQEV
                     DNCDYHGMILFGGEGYSFHSFLGLTDYFAKTIGDDLIGYHLNGAIINGVPWGDLTHTE
                     LIPGNSLYYNTFLIQPNPVADIISFPFNNGSFKIYNLNGQLVQQNLFSNSATSVNDLP
                     SGLYTIVVQSNGANYRSVFVKN"
     gene            3218..3460
                     /locus_tag="PLB46_09340"
     CDS             3218..3460
                     /locus_tag="PLB46_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76762.1"
                     /translation="MNDYTPISCSFYDILESIATKRELAIIEYFDDAKQQISVTGKIQ
                     NLFTQEKVEYMHLEQGLLIRLDNIIAVNGMRPGNYC"
     gene            complement(3465..4562)
                     /gene="ychF"
                     /locus_tag="PLB46_09345"
     CDS             complement(3465..4562)
                     /gene="ychF"
                     /locus_tag="PLB46_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRF76763.1"
                     /translation="MGLKCGIVGLPNVGKSTLFNALSSAKAQAANFPFCTIEPNVGVV
                     TVPDNRLTQLEALVHPERVVPTTIEFVDIAGLVKGASKGEGLGNKFLANIRETDAIIH
                     VVRCFDDPNIVHVHGNVDPIRDKEIIDYELQLKDLETIETRLSKSQRAASIGDKDAKK
                     LTEVLTAYKAHLEQGKSARSLVIDEDGAKLIADLFLLTIKPVIYVCNVEEKDLKTEND
                     FVRKLRDAVKDEDAEVIVVSANIESEIAQLESFEDKQLFLEDLGLTESGLNVLIRAGY
                     KLLNLITYFTAGVKEVRAWTIHKGMTAPQAAGVIHSDFEKGFIRAEVIKYNDFIHYGS
                     EAACKEAGKLNVEGKEYVVVDGDLMHFRFNV"
     gene            complement(4740..>5453)
                     /locus_tag="PLB46_09350"
     CDS             complement(4740..>5453)
                     /locus_tag="PLB46_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF76764.1"
                     /translation="NYVTPVTDFSSTGDPIVKFTDLSINIPTAWEWDFDDGGTSTEQN
                     PTHTYLADGTYNVCLTSSNSEGDDLQCKMVTISNTPKTPVVDFSFSISGTTVTFTDLS
                     TNTPDYWSWNFGDGGTSTEQNPSHFFPSPDAYTVCLTAGNVAGESFTCKLVDFNSVNN
                     LVTLNFTLTPNPANDFVTITSAEQSKAVIMNNLGEILSTFDVNQTMQLDVTEYPSGLY
                     FIQLQTDNSRGIQPIVIQH"
BASE COUNT         1696 a          948 c         1041 g         1768 t
ORIGIN      
        1 gccaaacaag catggtacga tttatcgctt gcagttgacc cgaataatgc caacgttgtt
       61 gcgatgggcg gattacactt gtggcgttcg cgtaatgcag gtgaaacctg ggagcgtatg
      121 acaacaggtg gtttggatag tgtcctgatt agatttatgc atgtggacca acatgaaata
      181 actttccgta attcggatga agtgtatttt ggcaacgatg gcggtattta tcgaacaaca
      241 aatttcactg cggaacaacc gtttatttac gatagaaatt atggatataa tgtaactcaa
      301 ttttattctg ttgctgtcca tcctacggaa ggtgtttcgc aattgatggg aggtacacaa
      361 gataatggaa caccctatac ttatgactca ggaaatgcgc cttttaaaat ggtgtcagga
      421 ggcgacggtg cttttacagc atatgatcac gaaaaaccgg aggtatttta tacggcatca
      481 caactcaaac gattattccg atttaataat ggtggttttg aaatgccgga cacaattacc
      541 aatccgaatt tagaagatta taatacttta tttataaatc catttgatat tgatgccagt
      601 gaccctgaag ttatttatca atgttcgaat atcggattaa tgcgtttaaa aaatgcatca
      661 acagccgata ctgggcaatg gctaaaatgc gtaaacctaa atggtgtgct ttctgcaatt
      721 gcttcatcac ctgctgctcc cggttcgtta tttattggta aaagttcagg ttcgggcgac
      781 atatatcgtt tagatgattc ttataattca gatgctactg tagtgccatt taatttggac
      841 ccatttgatt cattacctga tgccggattt ttaagtacag tttattgcag tagtattgtt
      901 gttgatgtaa atgatgctaa tcatgttatt gttacttact caaattataa tgtaaaaagt
      961 gtttgggaaa gttttaatgc gatggctttt gaaccaactt ggcattgtat tgaaggcaat
     1021 ttacaggata tgccagttta ttggagtgct atacatcctg attttccgaa tattgtttat
     1081 ctggcaacag aattaggtgt attttacaca aataatgtca acggatattc aactaattgg
     1141 atgccatgta ccaattttcc aattgtgaga acggatatgt tgcgcattaa aagtgctgaa
     1201 caattattag ttgctgctac acatggacga ggtttatggc aggcacaatt atcagcaacc
     1261 ggaacattaa ataatttgat ttggcaggag agaggtccca ataattttgg cggcagaact
     1321 cgcaccatca tgattgatcc aaacgaccct tcagggaaaa cagtttgggc cggtagtgtt
     1381 ggtggcggat tatggaaaac aactaatatt gatgctgtgc ccattataca acctgaaata
     1441 gttgaatcaa tttcattaca agtgtatccc aatcctgccg cggattatat caatttgcat
     1501 gtaacatttg cacaaccttc aaattcaaca attagtctat tggatatgca aggaaatatt
     1561 attgcggaaa taccgtctgc aaaaggtaca gctcagcaaa taataaaatg gaacattccg
     1621 gcaaacctgg gtgctggtaa ttattttatt atggtgcgtt caggacaaca aaaaatggtt
     1681 aaaaaaatta ttatcctata aacccttgac catgaaaaaa ctaatttgta ttgttttgat
     1741 tattgcgtcc tcatctattt tcgctcaaga ctggatgcct tttattccga atgcaaatca
     1801 ttttatttca catgaatctg tatataacct taatcaggat tccacggtga ttgaaggcga
     1861 catgacccaa tattttttta acaggcattt ccctgaccct gaaatggaat catgttttga
     1921 ttcaatagat gatattgagg aggagtattt tgttatagat agatgtgatg taattacaga
     1981 tacgttttat ttttattcag catatacccc gggctttaaa gttaaattta ttaatcctca
     2041 aacgataggt tattcctggg atgtgccaaa tactaatccg gtttcggatt ggtcgtttgt
     2101 tacgatatcg tatgattcaa taggcactga aacagttttc gatgtggtag atagcgtaaa
     2161 atatttttct ataaatgtag atgttgaatt acctgattcg gatgcaatag ataaagtggt
     2221 aattaaattg agtaaatcaa acggttttat tcaatattta aattggttca gatttgaaaa
     2281 tccacgattg ttgtatcctt attatactga gttgttggag gggtattccg ttgatgggat
     2341 aaataaaggg tttgttatcc ccgaatggga tgattatttt cattatgtag aagatgatgt
     2401 gttgatttgg aaagctacgt atcccaatgg aagtatacgc tattttaccg acaccgtttt
     2461 gaatatttac aaagatgatg aactagtgga attaattaca acaaacgatg tattcacttt
     2521 ttataaagag gcataccaat acgttcttgg aggaccacta tgtaacgtga tgtatagtcc
     2581 ggcatcttta aatgatgaaa gttttgaaaa ttcgggcatg tactccttag gttttgtaat
     2641 caatcatata gataaaagtg gattaaatcc ccaaattagt aaacaaattt atactgatag
     2701 aatacaagaa gttgataatt gtgattatca tggaatgata ttatttggcg gtgaaggata
     2761 ttcatttcat tcatttttag gattaacaga ctactttgcg aaaacgattg gcgatgattt
     2821 aattggctat catctaaacg gtgctattat taacggtgtt ccatggggtg acttgacgca
     2881 tacagaatta atacccggaa acagcctata ctacaataca tttttaatac agcctaatcc
     2941 ggtcgcagat ataattagtt tcccatttaa taatggttcg tttaagatat acaacctgaa
     3001 cggacaatta gtgcaacaaa acctgttttc taatagtgcc acatcagtaa acgatttacc
     3061 atccgggtta tacactattg tagtgcaatc caatggagca aattaccgct cggtgttcgt
     3121 aaaaaactaa atgcactgct agtcagcaaa tgaattgtac ctatttgaaa attggtataa
     3181 accggaactt ttcaggtaat taaatagttt tgtaataatg aacgattaca cgcccatcag
     3241 ctgtagtttt tacgacatct tagaatcaat tgccaccaaa cgggagcttg ccataattga
     3301 atattttgat gatgctaagc agcagatttc agttaccggt aaaattcaaa atttgtttac
     3361 gcaggaaaaa gtggaatata tgcacttaga gcagggtttg ctcattcgtt tagataatat
     3421 tattgctgta aatggtatgc gcccaggtaa ttactgttag ttgcttatac gttaaaacgg
     3481 aaatgcatta agtcaccatc cacaacaaca tattctttac cttccacatt aagtttacct
     3541 gcttctttac acgcagcttc agagccgtaa tgaataaagt cgttgtattt aataacctct
     3601 gcacgaataa atcctttttc aaaatctgaa tggattacac ctgcagcttg cggagcagtc
     3661 attcctttgt gaatagtcca tgcgcgaact tctttcacgc cggcagtaaa gtaggtgatc
     3721 agatttaata atttatatcc ggcgcgaata agtacgttta aaccactttc ggttaacccc
     3781 aaatcttcaa gaaataattg tttgtcttcg aaactttcta attgcgcaat ttcactttca
     3841 atattggcac ttactacaat tacttcggca tcttcgtctt ttacagcatc acgtaatttg
     3901 cgaacaaaat cattttctgt ttttaaatct ttttcttcca cattacacac ataaattacc
     3961 ggtttaatgg tgagtaaaaa taaatcggca attaatttag caccatcttc atcaatcact
     4021 aaactacgtg cacttttacc ctgttcgaga tgtgctttgt aggcggtaag tacttctgtt
     4081 aattttttgg catccttatc accaatactt gcggcacgct gacttttact caaacgtgtt
     4141 tcaatggttt cgaggtcctt taattgtaat tcgtaatcaa taatttcttt atcacgaata
     4201 gggtcaacat taccatgcac gtgtacaata ttagggtcgt caaaacatct aacaacatga
     4261 ataatggcat ccgtttcacg aatgttagct aaaaatttat tgcctaaacc ttcgcctttt
     4321 gatgcgcctt ttaccaaacc tgctatatca acaaactcga tagttgtagg tacaacgcgc
     4381 tcaggatgaa ccagggcttc taattgtgtt aagcgattat ccggcacggt tacaacgcca
     4441 acatttggct caatggtgca gaacgggaaa ttagccgcct gagcttttgc acttgaaagt
     4501 gcattaaata aagtcgattt gcccacattc ggaaggccta caattccgca ttttaaaccc
     4561 atgatgataa attttaaggc ggcaaagata cggatttgtt gcaagaaact cagcagagac
     4621 aaagaagttg gtcatactga tacagacccg ggagtcttgt tgtgctgttt tgatagacta
     4681 gagcataaaa aaaccgcctc tttcgaagcg gttttgtgta ttgatagttg gtttggaaat
     4741 caatgttgaa taacaatagg ttgtatgccc cggctattgt cagtttgtaa ttgaataaaa
     4801 tacaaaccgg aaggatattc ggttacatcc aattgcattg tttgattaac atcgaaggta
     4861 gataaaattt ctccaaggtt attcataatt actgctttgc tttgttccgc tgaagttatg
     4921 gtaacaaaat cgttggccgg atttggtgtc agcgtgaaat ttaatgttac caaattgttg
     4981 acactattaa aatcaaccaa tttacaagta aagctttcac ccgcaacatt acctgcagtt
     5041 aaacacaccg tataagcgtc aggtgatggg aaaaaatgcg atggattttg ttctgttgaa
     5101 gttccaccat ctccaaaatt ccatgaccaa taatcaggag tattggtaga taaatcggta
     5161 aaggttacag ttgttcctga tattgaaaaa ctaaaatcaa ctacaggtgt ttttggtgta
     5221 ttactaatag tcaccatttt acattgtaaa tcatctcctt ctgaattgga tgatgttaaa
     5281 catacattat aggtgccatc agcaagatat gtatgtgttg gattttgttc tgttgaagtt
     5341 ccaccatcat caaaatccca ttcccatgca gttggaatat ttatagataa atctgtaaac
     5401 tttacaatcg ggtcaccggt actagaaaaa tcagtaaccg gagttacata att
//
LOCUS       DAORKW010000215         5446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_602_length_5446_cov_5.384901, whole genome shotgun sequence.
ACCESSION   DAORKW010000215 DAORKW010000000
VERSION     DAORKW010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_602_length_5446_cov_5.384901"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1930
                     /locus_tag="PLB46_09355"
     CDS             62..1930
                     /locus_tag="PLB46_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76765.1"
                     /translation="MKKLQSILTILIMLGLTSVAHAQLPTKSLTGEISGITRNLVNDT
                     VYILTGFVYVEDGATLNIEAGTIIRGDKDTKGSLIVTKGSKIYALGTPDQPIVFTSNQ
                     PEGSRNYGDWGGIIILGDAPINVAGGTAVIEGGVDTPEGDAVYGGADATDNSGSLTYV
                     RIEYPGIAFVPGNEINGLTCGGVGSGTILDHIMVSRSGDDAFEFFGGTVNGKYLIAHN
                     TYDDDFDTDFGFQGKIQFGLVKRDPNFADVSGSNGFESDNDATGSTNSPQTNPTFSNM
                     TVVGPIQNAGDVINVNYKRGAHLRRNTNTDIFNSVIIGYPSGIMVDGALCEASATGNT
                     LKVQNTIIAGHNNDFEVAVGSSFDANAWFTTGAYNNSILDLSSDVMLTDAYNITSPNF
                     LPMGGSPLLSGASFVESELIGMIPTTYKGAFGNLDWTSCWANWDPQNTNYGEVPGTIA
                     PAIASFDISNADLTVTFNNTSSNAVSYSWDFGDETTTEDVSTEANPSYTYPALGVYNV
                     VLTATGTCADQNTTSQSLDLSVAITDFNNINSVSVYPNPAKNAATVAISATSNTTADI
                     TVTDMTGRVILPTISANLMSGNNAYTFNTSTLSSGVYLVNVTNAGENAALQLIIAK"
     gene            2189..2539
                     /locus_tag="PLB46_09360"
     CDS             2189..2539
                     /locus_tag="PLB46_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRF76766.1"
                     /translation="MHITPANALHTLHQSNKLFISLFEHGSLVVEIYQPQKVDLQQPH
                     DRDEVYVITNGHGQFNHNNTIVSVNTGDFLFVPAGDPHHFFDFSDDFATWVIFYGPKG
                     GELNTFTHENENYL"
     gene            2766..3239
                     /locus_tag="PLB46_09365"
     CDS             2766..3239
                     /locus_tag="PLB46_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF76767.1"
                     /translation="MHANEQLIHNFYSAFLDKDYLTMQNSYAEEATFSDPVFQHLSVQ
                     EVRAMWEMFCKRGKDMQLTFSIQHADDKYVHATWIPNYTFSATGRLVTNYITSKFTIV
                     DKKIVNHVDHFNFYKWSSQALGLSGMLLGWTPMVKNKVRKTAMKNLHTFMQKSAS"
     gene            3244..3792
                     /locus_tag="PLB46_09370"
     CDS             3244..3792
                     /locus_tag="PLB46_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF76768.1"
                     /translation="MSIPATALPELITSRLILNQVQIHDAPQVFILRTDKLVNKYVVL
                     PETPDEAAAANFIHKVNDYNLQNKGRYWAIRLKDNPELIGTICLWNFDDVANTSELGF
                     TLLTDYWRKGYMQEALQCVLQYGFDDLHLTKIDGWTHQLNEASKGLMEKVGFTRNVAL
                     EKQVIIDESEKHMVIYTILNPN"
     gene            complement(3789..4685)
                     /locus_tag="PLB46_09375"
     CDS             complement(3789..4685)
                     /locus_tag="PLB46_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRF76769.1"
                     /translation="MKFAAIDIGSNAIRLLIENVYETPKGPVFHKDSLIRVPVRLGED
                     AFIDGVISEEKTQMLVKAMISMKHIMEIHKVTAFKVCATSAMRDAKNSDEILARVKKE
                     AGLEIQIISGDREGKTILGVPLDQIGLEPNDFYLYIDVGGGSTELAFLGNGKLIANRS
                     FNIGTLRLLNDQVNDPEWREMLEWIKATKNNNFELSAIGVGGNINHVMKHYGKAGKPY
                     LSISILKRVMMYMDDLSMGERVRELGLKPDRADVIVPALKIYINVMKWAGIEKIFVPK
                     QGLSDGLISQLYHEWKSKTIEV"
     gene            complement(4698..>5446)
                     /locus_tag="PLB46_09380"
     CDS             complement(4698..>5446)
                     /locus_tag="PLB46_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRF76770.1"
                     /translation="EGKQVQYAIIGTGNFNENTAKVYTDIFLLTARPEITKEVKKIFE
                     FFDNRFEIGKYKHLMVAPLNMRAKMKQLISREIKNAKDKKPALLFFKLNNLVDKEIIK
                     KLYEASNAGVKVKLIVRGICSLVPGVEGLSENIEAISILDRYLEHPRIFYFYNNGKEE
                     IYMGSADMMERNIDHRIEVLVSILDKDIKKQLKEILELTWKDNVKARTLVHGHLNDYV
                     TKAKNTEALRSQLALYNYFKSYYEKLKTNN"
BASE COUNT         1698 a         1023 c          945 g         1780 t
ORIGIN      
        1 gatagcggtg caaaaatttc tttatctgtt aattataaat tctaaataaa aatttcaaat
       61 tatgaaaaaa ctgcaatcaa tcttaacaat tttaattatg ctgggtttaa caagtgttgc
      121 ccatgcacaa ttaccaacaa aaagcctgac aggtgaaata tcaggcatta cgcgcaatct
      181 cgtaaacgat actgtgtata ttctaacagg attcgtttat gttgaagatg gtgcaacttt
      241 aaatatcgaa gcgggtacta ttattcgtgg cgataaagac acaaaaggaa gtttgatcgt
      301 tactaaaggt tctaaaattt atgctttagg aactcctgat caaccaattg tatttaccag
      361 taatcaaccg gaaggttcac gtaattatgg cgactggggt ggaattatca ttttaggtga
      421 tgcaccaatt aacgttgccg gtggaactgc agtaattgaa ggtggagtgg atacacctga
      481 aggtgatgct gtttatggtg gagctgatgc aaccgataat tcaggttcat taacgtatgt
      541 gcgtatcgaa tatcccggta tcgcatttgt tcctggaaat gaaatcaacg gtttaacttg
      601 tggtggtgtt ggttcaggca ctattttaga tcatattatg gtaagccgct ccggtgatga
      661 tgcatttgaa ttttttggtg gaacagtaaa tggcaaatat ttaattgccc ataatactta
      721 cgatgatgat tttgatactg attttggttt tcaaggcaaa atacaattcg gattagtaaa
      781 acgcgacccg aattttgctg acgtttcagg ttctaatggt tttgaaagtg ataatgatgc
      841 aactggtagc accaattcac ctcaaaccaa tccaacattt agtaatatga cagtagttgg
      901 tccaatacaa aatgcaggcg atgtaattaa tgtgaattac aaacgtggtg cacacttacg
      961 cagaaatacc aatactgata tttttaattc cgtaattatt ggttacccaa gtggaattat
     1021 ggttgacggt gctttatgtg aagctagtgc aactggtaat actttaaaag tacaaaatac
     1081 cattattgcc ggacacaaca acgattttga agttgctgta ggaagtagtt ttgatgcaaa
     1141 tgcttggttt accaccggtg cttacaataa tagtatttta gatttaagca gcgatgtaat
     1201 gttaactgat gcttataata ttacttctcc taactttctg cctatgggtg gttcaccatt
     1261 attaagtggt gcatcatttg ttgaatcaga attaattggt atgattccaa caacttacaa
     1321 aggtgcattc ggtaatttag actggacttc ttgttgggct aactgggatc cacaaaatac
     1381 taactacggt gaagtaccgg gaacaattgc tcctgctatt gcatcgtttg atatttccaa
     1441 tgctgactta acagttacat ttaataatac atcaagtaat gccgtatctt attcttggga
     1501 ttttggtgat gaaactacaa ctgaagatgt aagtactgaa gctaatcctt catatactta
     1561 tcctgcatta ggcgtgtata atgttgtgtt aactgcaaca ggcacttgtg cagaccaaaa
     1621 tactacatca caatcattag acctttcagt tgctattact gatttcaata atatcaacag
     1681 tgtaagtgta tatccgaatc ctgcgaaaaa tgcagccaca gttgccattt cagctacttc
     1741 gaatacaaca gctgatatta ctgtaaccga catgacagga agagtaatat taccgacaat
     1801 ttcagccaat ttaatgagcg gaaataatgc gtatactttc aacacttcaa cattaagttc
     1861 aggtgtatat cttgtaaacg ttactaatgc aggcgaaaac gctgcattac aattaatcat
     1921 agctaaataa gttttcgtat tgtaagaaag gggtcgccgc gaggtggcct tttttttgta
     1981 cctacatgtg taattttgta actagaactc atctcaaaaa taggtttgtt ttagtatttt
     2041 caagtattcg ctctttagcc gaggcgcttg aagcagccca taccggaaga aggacgggca
     2101 attcaagcaa cgaaggataa agagcgaaga cgaagaaaag acgccaaagg tatttttgag
     2161 atgagttcta attacaaata caaatctcat gcatattaca cctgcaaacg cattacatac
     2221 tttacatcaa tcgaataaat tatttatttc gttgtttgaa catggcagtt tagttgtgga
     2281 aatatatcaa ccacaaaaag tagacttaca acaaccacac gaccgtgatg aagtatatgt
     2341 gattacaaat ggccatggac aatttaacca caacaataca attgtatcag taaataccgg
     2401 agatttttta tttgtccctg caggtgaccc tcaccatttt tttgattttt ctgatgactt
     2461 tgcgacctgg gtaatatttt atggcccaaa aggcggagaa ctaaacacat tcacacatga
     2521 aaatgaaaat tatttgtgaa cattgtaatg ttgcgttaca acccgaaggt gtagcctttg
     2581 tttgtagcta cgaatgcacg tattgcagta attgcgccac tcagtttcag catatttgcc
     2641 caaattgtgc aggcgaattg gtccgccgcc ctaagcgatt gccaaaagag caaacaagct
     2701 aaatatttca atcgtttgat tttctcactg cattccggta aatttacttt actcaaagaa
     2761 tttttatgca cgctaacgaa caattaatcc ataattttta ttccgctttt ttggataaag
     2821 attatctgac tatgcaaaat agttatgccg aagaggctac cttttccgac ccggtatttc
     2881 agcatttatc agtgcaagaa gttagggcaa tgtgggaaat gttttgtaag cgtggaaagg
     2941 acatgcagct cactttttcc attcaacatg ctgatgataa atatgtgcat gccacctgga
     3001 ttccgaatta tacattttct gcaaccggca gacttgtcac caactatatc acatcgaaat
     3061 ttactattgt cgataaaaaa atagttaatc atgtcgacca tttcaatttt tataaatggt
     3121 caagtcaggc attaggtttg agcggaatgc tactcggctg gacaccaatg gtgaaaaata
     3181 aagtgcgcaa aacagcaatg aaaaatttac atacttttat gcaaaaatca gcttcttaaa
     3241 aatatgtcaa ttcctgctac tgctttaccc gaattaatta cttccagatt aatactcaat
     3301 caggttcaaa ttcatgatgc tccgcaagtg tttatcttac gaaccgataa attggtaaac
     3361 aaatatgtcg ttttacctga aacaccggat gaagccgcgg ctgctaactt tattcataaa
     3421 gtaaatgatt acaatttgca aaataaaggc agatattggg caatacgttt gaaggataat
     3481 cctgaactta ttggtacaat ttgtttatgg aattttgatg atgtagccaa tacatcagaa
     3541 ttaggattta ccctgttgac tgattactgg cgtaaaggat atatgcagga agcgcttcaa
     3601 tgtgtgcttc agtatgggtt cgatgattta catcttacaa aaattgatgg atggacccat
     3661 caactcaatg aagcatcgaa agggttaatg gaaaaagtgg gttttactag aaatgttgcg
     3721 ttagaaaaac aagtcatcat agatgaatcc gaaaaacata tggtaattta caccattcta
     3781 aatccgaact aaacttctat tgttttgctc ttccattcat gatacagttg cgatatcaat
     3841 ccatcgctta atccttgttt tggtacaaat attttttcaa taccggccca tttcatcacg
     3901 ttgatataaa ttttaagcgc tggcacaata acatctgccc tatccggttt taaacctaat
     3961 tcgcgaacgc gttctcccat actcaaatca tccatgtaca tcataacacg ctttaatata
     4021 ctgatgctca aataaggttt accggcttta ccataatgtt tcattacatg attgatattt
     4081 ccgcctacac caatggcact taattcgaaa ttgttatttt ttgtagcctt aatccattcc
     4141 aacatttcgc gccattcagg atcatttacc tggtcgttta acaaacgtaa agtcccaata
     4201 ttaaatgaac ggttagcaat taatttacca ttgcctaaaa aggctaattc tgtgcttcca
     4261 ccaccgacat ctatgtataa ataaaaatca tttggctcta aaccaatttg atccagtgga
     4321 acacccaaaa ttgtttttcc ttcgcggtcc cccgatataa tttgaatttc aagtcctgct
     4381 tcttttttta cacgtgctaa tatttcatca ctgttttttg cgtcacgcat ggcagaagta
     4441 gcacacactt taaaagcagt taccttgtga atttccatga tatgtttcat agatatcatt
     4501 gcctttacca acatctgtgt tttttcctca gaaattacac catcaataaa tgcatcctca
     4561 cctaatcgaa ccggtacacg aattaacgaa tccttgtgaa aaacgggacc tttaggtgtt
     4621 tcatatacat tttcaatcaa caatctgatt gcattacttc ctatatctat agctgcaaac
     4681 ttcatttctt aatctgatta attattagtt tttaatttct cgtaataact tttaaaataa
     4741 ttatataacg ccaactgact cctaagtgct tctgtatttt tagcttttgt aacgtaatca
     4801 ttcaaatgac catgcactaa ggttctcgcc ttcacattgt ctttccaggt tagctctaaa
     4861 atttctttga gttgtttttt gatgtcttta tctaaaatac tcaccaatac ttcaatccta
     4921 tggtctatat ttcgttccat catatcggct gaacccatat aaatttcttc tttgccattg
     4981 ttgtagaagt aaaatatacg cggatgttcc aggtaacggt ccaaaatgct aatggcttct
     5041 atattttcgc ttaatccttc aacacccgga accaatgagc aaataccgcg aacaatgagt
     5101 ttcaccttta cccctgcatt acttgcttca tataattttt tgataatttc tttatccacc
     5161 agattgttca acttgaaaaa caacaacgca ggttttttgt ccttggcatt ttttatttcg
     5221 cgactaatca gctgtttcat tttggcacgc atattcaacg gcgctaccat caggtgttta
     5281 tatttgccga tttcaaaacg attatcgaaa aattcgaaaa tcttttttac ctctttagta
     5341 atttcaggtc tggcagtcag taaaaatata tcggtataaa cttttgctgt attttcatta
     5401 aagttaccag ttccaattat ggcatattgc acttgtttac cttcat
//
LOCUS       DAORKW010000216         5389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_622_length_5389_cov_5.622610, whole genome shotgun sequence.
ACCESSION   DAORKW010000216 DAORKW010000000
VERSION     DAORKW010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_622_length_5389_cov_5.622610"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLB46_09385"
     CDS             complement(<1..391)
                     /locus_tag="PLB46_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF76771.1"
                     /translation="MNKTYLLVLCLLVQLQLSGQTDSLRSTISGIITGKSAVVGVQIT
                     EIETGASVTLNPEYKYPLMSVFKLHLGVTVLHAVDEGKLTLEQGVLIKKSDLMENTWS
                     PIRVKYPEGNVTLSIKELLQYTIADSDN"
     gene            complement(388..543)
                     /locus_tag="PLB46_09390"
     CDS             complement(388..543)
                     /locus_tag="PLB46_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76772.1"
                     /translation="MGKIRMPLLAFMNFRKNVAYVIPVLRFSVKRGFAAQKIYLCLAQ
                     YHSTSIT"
     gene            591..1298
                     /locus_tag="PLB46_09395"
     CDS             591..1298
                     /locus_tag="PLB46_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76773.1"
                     /translation="MKKITIAVCSILFLIKSLSAQEIDVAVGVGTILPGENYCAKLRA
                     DLLIENHFSVGVQTNFYADKFTTSGFILEPNMRIYFNNVHQKNVFYWQLSGFAGQVEH
                     VQLYAHEIEDDVLETTFYPYLDLFTQLFFETIENKEYIFHTRTYDIQISGMGAAFGYK
                     RDFGLKNNLFVDMNFGFKAIRQPEYIAEHVTVDGITFGRAGENIFNNNLPEQYRIVED
                     SRALRTVTGFLGIGYRF"
     gene            1409..2881
                     /locus_tag="PLB46_09400"
     CDS             1409..2881
                     /locus_tag="PLB46_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF76774.1"
                     /translation="MKTIFYLVFSLLVLSANGQQKIFSVYFQNDEYQLDEDDIQIIKE
                     AAAFVAQPGIIASGIQINGYTDAPATADYNVNLSHKRALAVKELFQSKIESDIKISLA
                     WHGEIIPEATTDLHYGQRCADIVITYTEQKQIVQVPKSDINELWKQLREPAQVFTINP
                     QKDTLIMGEEGTIINIPAGAFIIPEEKQGRQVNIVLDEYYDYASMIINNMTTMSSGLP
                     LETGGMIYVNAFLDNRALTVMEDKPLTFMFPGDTMVTGMQFFTGNTNPTGNMNWLLTE
                     TDLETITPDEYHNLIYPTGFTKKRYRKNCPYLFCGLREDLGADNKKYYEKRAYMATDW
                     KAYRKYTDSLCLVYGVRNFGVLKRMLLQQVRQTEDPFFYVAKTSRLGYMNCDKFYKLP
                     PEKLTLLAINEAPAENVNSFLVFSKYKSLMLPNTNNPEKAEFSNIAKKSKCKAVVIKY
                     ENKTAYLSVQEIKAGDVPEVTPEFVAMTPAQLKTELKKLK"
     gene            complement(3037..>5389)
                     /locus_tag="PLB46_09405"
     CDS             complement(3037..>5389)
                     /locus_tag="PLB46_09405"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRF76775.1"
                     /translation="AITMNPTNKFLLALCVLLSTTAIFAQTNLFPTTGSAGIGTTDPN
                     PSALLDVVSTSKGILVPRMTKVQRDAIASPATGLLIYQTNVNPGFFYFNGTAWTAISS
                     QDASKSLNNLTTTAVNINLQPGTDNTIDLGSAAQSWKNLYVDGIGYLGTAKLGNYVGT
                     PEAGMIRWNGTDFQGYNGISWTSLSGGGGGGGDVATDLSNLTSTSINVDLIPNIDAIH
                     DLGSTTKSWDNIYIENQLFIGSTPAMKIDGDASYPSIFIGNTGHEDFIGNDNIFIGRS
                     TASAATSTAYNNIAIGAYAGSSNNDGDENVFIGSSAGSANTSGRRNLSFGSGAGQLTT
                     TGDENIFLGQAAGYGNITGNKNICIGSVTHSNTDIGGNDSLNVIVGNRAGSYNAGSET
                     VLIGAFAGHGNIQNYNVYIGAHAGFSSVNGYDNVYIGRNAGYTQVDGYANTFVGENAG
                     YNFGGSGTSSRYNVYMGTDAGYYTKGAYNTYIGSKCGDSYADNEQCVLMGYGTDVSSA
                     TITNAIAIGYFTSISQSNSIRLGNSSINKIGIGRNTSAGSIMEFQATTAKLTTGGVWT
                     DASDKRLKRNITELNKAEILDKINKLSVTEWNYIADDPNIKHIGPMAQDFYAAFGLGD
                     DTTIAAMDKAGVALIGIQELSSKLASLNSVVETVTAENKLLQERIEKLENVISGNPTS
                     NENSNAQDIILAEESDMPVLFQNKPNPFTGNTTINYFVPTSAKTAILQIVNQQGVIIS
                     TIQLAIGSGNIEIDATQLNSGLLTYSLIVDEKIISTKQMMLTK"
BASE COUNT         1655 a          989 c          980 g         1765 t
ORIGIN      
        1 tattatcgct atcagctatg gtatattgta aaagttcttt tatagacaat gttacattcc
       61 cttccggata ttttacccgt atcggactcc aggtattttc cattaggtcg ctttttttga
      121 tgagtacacc ttgttcaagg gtcagcttgc cttcatcaac ggcatgtaaa accgttaccc
      181 ctaaatgtaa tttaaaaaca ctcatcaatg gatacttgta ttccggattt aatgttaccg
      241 aagccccggt ttctatttcc gtaatctgaa caccaaccac agcgctttta cccgtgatga
      301 tgccggaaat ggtagacctt aaagaatctg tttgtcctga cagttgtaac tgaacgagca
      361 aacacagtac taatagataa gttttgttca tgtaattgaa gttgagtggt attgagctaa
      421 acaaaggtaa attttttgag cagcaaatcc tcgttttacg gaaaatcgca aaaccggaat
      481 aacgtaagct acatttttgc ggaaattcat aaacgccaat aacggcattc ttattttacc
      541 catattaccg gtttttggca attctacatt tgcatcataa aaatattgtt atgaaaaaaa
      601 ttacgattgc tgtttgttct atcctattcc taattaaaag tttatctgct caggaaattg
      661 atgtggctgt tggtgtgggt accattttgc caggtgaaaa ttattgtgct aaactacgcg
      721 cggatttatt aattgaaaat catttttctg tcggcgtgca aactaatttt tatgcagata
      781 aatttacaac ttcaggcttt atacttgagc ctaacatgcg catttatttt aataatgttc
      841 atcagaaaaa tgttttttat tggcaactct ccggctttgc agggcaggtt gaacatgttc
      901 aactttatgc gcatgaaatt gaagacgatg tgttggaaac aaccttctat ccatatttag
      961 atttgtttac gcaattattt tttgaaacaa tcgaaaacaa ggaatatatt tttcacacac
     1021 gtacttatga tattcaaatt tcaggtatgg gtgccgcctt tggatataag cgcgacttcg
     1081 gtttaaagaa taatttgttt gttgatatga atttcggctt taaggctatt cgacaacctg
     1141 aatatattgc tgaacatgta acggttgatg gtatcacctt tggtagagcg ggtgaaaata
     1201 tttttaacaa caatctccct gaacaatatc gtattgtaga agatagcagg gctttgagaa
     1261 cggttacagg gtttttaggt atcggatatc gtttttaatt ttttttatgg tttggttggg
     1321 ccgcagtcct ttaatgggtt gcggccattt tggttttgac gggatactaa agcatcacaa
     1381 tttttcgttt tgccatcaaa cacataagat gaaaacaata ttttaccttg ttttttcttt
     1441 acttgtactt tctgcgaacg gtcaacaaaa aatatttagt gtttattttc aaaatgatga
     1501 atatcagctc gacgaggacg atattcaaat aattaaagag gccgctgcat ttgttgcaca
     1561 gcccggaatt attgcttccg gtattcagat aaatggatac actgatgcac ctgcaacggc
     1621 agactataat gtcaacttat cgcataaaag agccttagcg gtaaaggaat tattccaatc
     1681 taaaatcgaa tcggatatca aaataagttt agcctggcat ggagaaataa ttcctgaggc
     1741 tacaaccgat ttacattacg gtcagcgatg cgctgatatt gtaattactt atacagaaca
     1801 aaaacaaata gttcaggttc cgaaatcgga catcaacgaa ttatggaaac aattgcggga
     1861 acctgctcag gtatttacca tcaaccctca aaaggataca ttaataatgg gagaggaggg
     1921 cacaattatt aatattcctg caggcgcatt tattattccg gaagaaaaac aaggtcgaca
     1981 agtaaatatt gtgttggatg aatattatga ttatgccagc atgatcatta acaatatgac
     2041 tacaatgagt agcggtttac cattggaaac cggcgggatg atttatgtaa atgcattttt
     2101 agataatcgt gcgctgacgg tgatggaaga taaaccatta acatttatgt ttcccggtga
     2161 tacgatggtt acgggtatgc aattttttac cggtaatact aatccaacag gtaatatgaa
     2221 ttggttgctt acagaaacgg atttagaaac aataacaccg gatgaatatc acaatctaat
     2281 ttatccaaca ggttttacta aaaaacgata tcgtaaaaat tgcccttatt tattctgcgg
     2341 attaagggag gatttaggtg ccgacaataa aaaatattat gaaaaacgtg cttacatggc
     2401 aaccgattgg aaagcctata gaaaatatac ggatagttta tgtttggttt atggtgtgcg
     2461 caattttggt gtattaaaac gaatgttgtt gcaacaggta agacaaactg aagacccatt
     2521 tttttatgtt gcaaaaacca gccgtttagg ttacatgaat tgtgataagt tctacaaatt
     2581 accacctgaa aaacttaccc tactcgctat aaatgaagct cctgcggaaa atgtaaattc
     2641 ctttttggta tttagcaagt ataaaagttt aatgttgccc aatacaaata accccgaaaa
     2701 ggcagaattt agcaatattg ccaaaaagtc taaatgtaaa gctgtagtca ttaaatatga
     2761 aaataaaact gcgtatttaa gtgtgcagga aataaaagcc ggagatgtgc ctgaagttac
     2821 gcctgagttt gttgctatga cacccgctca attaaaaact gagttgaaaa agctgaagta
     2881 acatttttaa ttgtaaagtt gattgcctta taggaaatgc ctttacgatt tatctattaa
     2941 ttcaaaacat gtttacaaac caataaacag catgtgttgc ctgtaaaaaa aagaggttga
     3001 aaattttcaa cctcttttta tgttgtgctg ctgagattat tttgtgagca tcatttgttt
     3061 agttgagatg attttttcgt caactattaa tgaataggtt aataaacctg aatttaactg
     3121 cgtagcatca atttcaatat tacctgaacc tatcgctaat tgaatggttg aaataattac
     3181 cccttgttga tttacaattt gtaaaattgc tgttttagca gaagttggaa caaaataatt
     3241 aatggtagta tttcctgtaa acgggttagg tttattttgg aataataccg gcatatcgga
     3301 ttcttcagcc aaaatgatgt cttgcgcatt actattttcg ttacttgttg gattaccgct
     3361 tattacattt tctaattttt caatgcgttc ttgtaataat ttgttttctg cagtaactgt
     3421 ttctacaaca ctattaagtg atgctaattt cgacgataat tcctgaatac caatgagtgc
     3481 aacacctgct ttatccattg cagcaattgt agtatcatca cctaaaccaa aagccgcata
     3541 aaaatcctga gccatagggc caatatgttt tatgtttgga tcatcggcta tataattcca
     3601 ttcggtgaca gacaatttat ttattttatc taatatttca gccttgttta attcagtaat
     3661 atttcgtttc aatcgtttat cactcgcatc ggtccaaaca ccacctgtgg ttagttttgc
     3721 ggttgttgcc tgaaactcca ttatactgcc ggctgatgta ttacgtccta taccaatttt
     3781 attaattgag gaattgccta aacgaattga attgctttga cttatagatg taaaataacc
     3841 aattgcaatt gcgtttgtta tggttgctga actaacatcg gtgccatatc ccatcaaaac
     3901 acattgttca ttgtctgcat aactgtcgcc acattttgaa cctatataag tattgtaagc
     3961 gcctttggta taataaccgg catcagtgcc catatataca ttgtatctgc ttgaagtgcc
     4021 gcttccacca aaattataac cggcattttc tcctacaaat gtgttggcat atccgtctac
     4081 ttgtgtgtac ccggcatttc ttccgatata tacattatcg tatccattta ctgaactaaa
     4141 tccggcatgc gcaccgatat atacattgta attttgaata tttccatgtc ccgcaaaagc
     4201 acctattaaa accgtctcag aacctgcatt ataacttcct gctctattcc caacaattac
     4261 atttaatgaa tcattgccgc caatatctgt attagaatgc gtcacagaac ctatacaaat
     4321 atttttatta cctgtaatat ttccataacc tgctgcctgg ccaagaaata tgttttcatc
     4381 acctgtggta gttaattgac ctgcacctga accaaatgat aagttgcgcc tgcctgatgt
     4441 atttgcagaa ccggctgagc taccgataaa aacattttca tcgccatcat tattactaga
     4501 acctgcatat gcaccaatcg caatgttatt atatgcagta gatgtggcag cggatgctgt
     4561 gcttcttcct atgaaaatat tatcgtttcc aataaaatct tcgtggccgg tattgccaat
     4621 aaaaatactt gggtatgaag cgtccccatc aattttcata gccggagttg atccaataaa
     4681 taattgattt tcgatgtaaa tattatccca acttttagtt gtgcttccca aatcgtgtat
     4741 cgcatcaata ttcggaatta agtcaacatt tattgacgtg cttgttaaat tagataaatc
     4801 ggtagctaca tcaccaccac ctccaccgcc gccggataaa ctcgtccaac taattccgtt
     4861 ataaccttga aagtcggtgc cattccaacg aatcattcct gcttccggtg ttccaacata
     4921 attgccaagt tttgcagtgc ctaaatatcc tatgccatct acatataaat ttttccagct
     4981 ttgtgctgca gaacctaaat caatagtatt atctgttccg ggttgtaagt tgatattcac
     5041 cgctgttgtg gttaaattat tcagcgactt acttgcatct tgtgaagaaa tggctgtcca
     5101 tgcagtgcca ttaaaataga aaaaaccggg gttcacattt gtctgataaa ttaataaccc
     5161 ggtagccggc gaagcaatgg catcgcgttg caccttggtc atacgcggaa ctaatattcc
     5221 tttagaagta ctcaccacat ccaataaagc agaagggttg gggtcagtag ttccaatacc
     5281 ggcgctgcct gtagtcggaa ataagttggt ttgagcaaat atggccgttg tggatagcaa
     5341 tacacataat gccaaaagaa atttgtttgt cgggttcatt gttattgcg
//
LOCUS       DAORKW010000217         5378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_625_length_5378_cov_4.693970, whole genome shotgun sequence.
ACCESSION   DAORKW010000217 DAORKW010000000
VERSION     DAORKW010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_625_length_5378_cov_4.693970"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..892
                     /locus_tag="PLB46_09410"
     CDS             167..892
                     /locus_tag="PLB46_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF76776.1"
                     /translation="MVVLPAYNAGKTLPQTYAEIPMDIVDEVVLVDDASKDDTIEVAK
                     RIGIKHIIAHEQNKGYGGNQKSCYNKALELGGDIVIMLHPDYQYTPKLITSMANIIAQ
                     DLYPVVLASRILGRGALKGGMPFYKYVFNRFLTLSQNLLMRQKLSEYHTGYRAFSADV
                     IRAINFEANSDDFVFDNQMLAQIFYNNFEIAEVTCPTKYFDEASSINFKRSVIYGLGV
                     LRTSLQYRLQKWGLVNYTYLRGK"
     gene            complement(1210..2064)
                     /locus_tag="PLB46_09415"
     CDS             complement(1210..2064)
                     /locus_tag="PLB46_09415"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRF76777.1"
                     /translation="MKKLNASRFGKERLLTNSKLKTKNFFAITKAAYKSWWSKEPFRQ
                     SAVIAYYAIFSIPGLLVLLISIAGYFLGKDVVSQHLMDQITSTLGKDTAIQLENAINS
                     TSAASISGWAKVLGVITIIIGATGVFSEFQRSLNLIWEVKPNKKKSRLWRLITVRLFS
                     FGLIVSIAFILIVSLAVSALITAFSHWISMHFSSTFYIIIQIINFSTTIGILALLFAL
                     IFKFIPDAKIKWRYTIIGSLVTAVLFEIGKFALEIYFGKTNPGDGYGAGGSIILILLW
                     VSYSSMIV"
     gene            complement(2009..3121)
                     /locus_tag="PLB46_09420"
     CDS             complement(2009..3121)
                     /locus_tag="PLB46_09420"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HRF76778.1"
                     /translation="MKKKYKVLIIIAVVLIVFRLILPFIVLHYANKTLSNMPGYYGHI
                     DDIDISLILGAYEIKDIYINKLDTVTHKESDFFNATSIDLSIDWPALFKGSIVGELEF
                     HTPTLVFIKTKTAIADLKADTTDFRNLLKSFMPLKVNKVAVDNGTFIYKDFNTTPNLD
                     LKLTNASALALNLTNVVNEDVKLPSTLTAKADIYDGIFILNMQLNALAETPTFDLNAS
                     LKNTHLPKLNDFFKAYGHFDVTDGDFSLYTELAASDGAFVGYLKPIIVNLNVVGPEDT
                     NDPILQKIWEHTVGGLGAVLKNQRENQVATKVDMNGTFDNPDVNSWEVILQVLRNAFI
                     QALLPTIDNEINIKSVNDDEETKRKPFWKRKAADKQ"
     gene            complement(3309..3965)
                     /locus_tag="PLB46_09425"
     CDS             complement(3309..3965)
                     /locus_tag="PLB46_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRF76779.1"
                     /translation="MKQLIQQHKTFYQSWLIWFVLVGLYLSFNSKASASLALNEIHTP
                     AGDILFRYITWGGDGILIGIVCFVFLFVRLRYGILLSLVSFISAFFVSLLKQFYDEPR
                     PSRYFAGQDVNWVDGIDLYANHSFPSGHAAAAFTMFLLMSFFFNNKSLTAVFFILALL
                     VAISRVYLFQHFLIDVWVGSFCGVVFGTILYHLIMITPLFKNKTWHNWSLLKSQNQLS
                     "
     gene            complement(3962..>5378)
                     /locus_tag="PLB46_09430"
     CDS             complement(3962..>5378)
                     /locus_tag="PLB46_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRF76780.1"
                     /translation="YIVKSYLGPFLIAFTIALIIFLMIFLWKYIEDLVGKGLEWYIIA
                     KLLFYASANMVPLSLPIAVLLACIMTYGNLGERFELAAFKSAGISMFTFMRSTIVIGV
                     LIAIGAFFFSNYILPSSYLKFVVTLVDITKQKPALNIKPNEFYNEIDGYSIMIGDKSD
                     DNITIEEVTIYEELDKNNDNVSIAEKGKMQTTKDNQNLIFTLYNGKQYEELQSDVKNK
                     KTAEHLRMNFGEWEKSIDLSGFQMTHTSENLYQNHYKMLNINQLAVAIDSLHHYNDRQ
                     STTLEPSMKMHYLQGRDSSAKIAAQPEVKLGKTKNFIDLIPVTERNAVVKKGLSTSRS
                     LAGYSNQVVQRLINTEMKIRDHQIEVYRKYTLSVACLLFVFIGAPLGSIIRKGGFGLP
                     VLLSIFIFIFYYILNIVGEKLAEQLVLTPFWGMWMGSFIIAPFSVLLTIKAKNDAPII
                     DGEWYYNQLMKFRQLFRRKQA"
BASE COUNT         1715 a          930 c         1020 g         1713 t
ORIGIN      
        1 cgatttccct aaaaacgatt aaacatctag tgaaggtttt tatcaaacaa gtgcccattt
       61 tgcaatggtt tcagccgttt ttggattaaa tatccagtga aatttcctgc cgaaatagtt
      121 agaaatcgta tttttgccgc caatcatgct gttaaataaa aaaatcgtgg tcgtattgcc
      181 ggcttataat gccggaaaaa cccttcctca aacctatgct gaaataccta tggatatagt
      241 ggatgaggta gtattggttg acgatgccag taaagatgac accattgaag tggctaaacg
      301 cattggtatc aaacatatca tcgcccacga acaaaacaaa ggatacggcg gcaatcagaa
      361 atcatgttat aataaagccc ttgaattagg tggtgatatt gtaattatgt tgcaccctga
      421 ttatcagtat acacctaaat taatcaccag catggccaat attattgcgc aggatttata
      481 tcctgtagtg ttggcatcac gcattttggg aagaggagca ttaaaaggtg gtatgccttt
      541 ttataaatat gtatttaatc gatttttaac cttatcgcaa aatttattga tgcgacaaaa
      601 gttgagtgaa tatcatactg gctatcgcgc attttctgca gatgtaattc gcgccataaa
      661 ttttgaagcc aactcagatg attttgtttt tgataatcag atgctggcgc aaatttttta
      721 taataatttc gaaattgctg aagtaacctg ccctaccaaa tacttcgatg aagcttcttc
      781 tattaacttc aaacgttcgg tgatttatgg gttgggagta ttgagaacct ctttgcaata
      841 tcgtttgcaa aaatgggggt tggtgaatta tacttattta agggggaagt gatggtttgc
      901 cgggaaggcg gggagacggg aagttgcggg gagttgttgt gatggtatct cgtgattgag
      961 tgggtttaaa ttaaaaccgg tttgggaatt attcctttgt gttacttcga ggcacttcga
     1021 gccttcgagg tgtcatttag aaaaactaaa attgtagcag catacattgt tgaaaaaaat
     1081 tgcttagatt taattttcaa cctgatgata ttattttttt tcctcctgtt tggcttcgac
     1141 cccggtatcg cttggctgaa tttgtgagac gtgtaaattg gcatatgctc tggtaaattc
     1201 tgcaccaaac tacacaatca tcgaggagta ggagacccaa agtaatatta aaataatgga
     1261 gccacctgcg ccatatccgt caccgggatt ggttttgccg aaatatattt ctaaagcaaa
     1321 ttttccaatt tcaaataata cagctgttac taaagaacct attatggtat atcgccattt
     1381 tattttggca tcaggaataa atttgaatat taaggcaaac aacaatgcta atatgccgat
     1441 ggtcgtggag aaattaataa tttgaattat gatataaaat gttgaagaaa aatgcatcga
     1501 aatccagtga ctgaatgcgg ttattaatgc agataccgct aaagaaacaa taagtataaa
     1561 tgcaatggat acaattagac caaatgaaaa taatcgcacg gtaattaatc tccacaaccg
     1621 cgatttcttt ttgttgggct taacttccca aattaaattt aaggaacgtt gaaattcgga
     1681 aaacacaccg gtggctccaa taattatagt gattacacct aaaactttcg cccaacctga
     1741 aattgaagca gcgcttgtgc tgttgatcgc attttcgagt tgtatagcag tgtccttacc
     1801 aagggttgag gtaatttggt ccattaaatg ctgactaacc acatcttttc ctaaaaagta
     1861 acctgcaatt gaaattagta atactaacaa tcccggtatg gaaaaaatgg cgtaataagc
     1921 aattactgca ctttgcctaa aaggttcttt gctccaccac gacttatagg ctgctttagt
     1981 aatcgcaaaa aagttttttg tttttagttt actgtttgtc agcagccttt cttttccaaa
     2041 acggcttgcg tttagtttct tcatcgtcat ttacggattt tatatttatt tcgttgtcaa
     2101 tagttggtaa taatgcctgt ataaatgcat tgcgtaatac ttgtaaaata acctcccaac
     2161 tattaacatc aggattatcg aaagttccat tcatatctac tttcgtagcc acttgatttt
     2221 cgcgttgatt ttttaaaaca gcacccaaac ctcctactgt gtgttcccaa attttttgca
     2281 gaataggatc attcgtatcc tcggggccaa ctacatttag gttgacaata atgggtttta
     2341 agtagccgac aaaagcacca tcggaagcag ccagttcggt atacagactg aaatcgccat
     2401 ctgttacatc aaaatggcca tacgctttaa aaaagtcgtt taatttaggt agatgtgtat
     2461 ttttaaggct ggcatttaag tcgaaagttg gtgtttcggc aagggcattt aattgcatat
     2521 ttaaaataaa tattccatca taaatatcag ctttggctgt gagtgtggat ggcagtttta
     2581 catcctcatt aaccacattg gtaagattta aggccaaagc agaagcatta gttaatttta
     2641 aatcgaggtt gggcgtagtg ttaaaatcct tataaataaa ggtgccattg tcaaccgcaa
     2701 ctttattcac tttaagtggc ataaacgatt ttaataaatt gcggaaatcg gttgtatcag
     2761 cttttaaatc tgcaattgca gtcttggttt taataaatac aagtgttgga gtatgaaatt
     2821 ccagttcacc aactatagaa cctttaaata aggcgggcca gtcaatggat aaatcgatac
     2881 ttgttgcatt aaaaaaatca gattctttgt gtgttacagt gtccaattta ttgatgtaga
     2941 tatcctttat ttcatatgcg cctaaaatga gcgatatatc gatatcgtca atatggccat
     3001 aatatccggg catgttggat aatgttttgt tggcatagtg cagcacgata aagggtaaaa
     3061 tcagacgaaa tacaattaat acaacggcaa taattattaa tactttatat ttttttttca
     3121 ttgataaatg gttttatacg tttagtgtaa attttacact tttggtatat tatcaaagtt
     3181 gcgcaataca tatgcagtgc gtgttataca aatgctttaa taatttatat aattcacatg
     3241 tttagggtga agggttgctg tttgttgggg atattgaata attgccggga aggcggggag
     3301 acgggaagtt acgagagttg gttttgggat tttaggaggg accagttgtg ccaggttttg
     3361 tttttgaaga ggggggtaat catgatgaga tgatatagga tagttccgaa aactactccg
     3421 caaaaggagc caacccacac atcgattaaa aagtgctgaa ataaatatac gcgtgatatt
     3481 gccactaata atgctaaaat aaaaaatacc gcagtgagtg atttgttatt aaagaaaaat
     3541 gacattaata aaaacatagt aaatgccgct gcagcatgtc cgcttgggaa actatgattg
     3601 gcatataagt cgataccatc cacccaattt acatcttgtc ccgcaaaata tctggatggc
     3661 cttggttcgt cgtaaaactg ttttagtagc gatacgaaaa atgctgaaat gaaagatact
     3721 aaactcaaca aaataccata tcgaagccgg acaaaaagaa atacaaaaca tacaatccca
     3781 attaaaatac catcgccacc ccaggtaatg taacgaaata aaatgtcgcc agcaggtgta
     3841 tggatttcgt ttaatgctag agaagctgat gcctttgaat taaatgataa atacaagccc
     3901 accaatacaa accatatcag ccaactttgg taaaacgttt tatgttgttg aattagttgt
     3961 ttcatgcttg tttacgacga aataattgac ggaatttcat tagttgatta taataccatt
     4021 caccatcaat tattggtgca tcgtttttag ctttaatagt cagtaaaaca ctaaatggtg
     4081 caataataaa actacccatc cacatgcccc aaaaaggcgt taatactaat tgttcagcca
     4141 atttttcgcc tacaatattg agtatgtaat aaaaaatgaa aataaatatg gatagcaata
     4201 ccggcaaacc aaatccgcct ttgcgaataa tggaacctaa tggagcgcca ataaatacaa
     4261 ataataaaca tgccaccgac aaggtatatt ttctatacac ttcaatttga tggtcgcgga
     4321 ttttcatttc tgtattaatt agtcgttgca caacctgatt gctgtaaccg gctaaactgc
     4381 gcgaagtgct tagtcctttt tttacaactg cattgcgttc agttaccgga attaaatcaa
     4441 taaaattttt tgttttgccc aatttcactt ccggttgtgc tgcaattttt gccgagctgt
     4501 ctctaccttg taaataatgc attttcatac ttggctccaa cgtagtgctc tgtctgtcgt
     4561 tgtaatgatg taatgaatcg atagccactg ctagctgatt aatattgagc attttatagt
     4621 ggttctgata aagattttcg ctggtatgcg tcatttgaaa accacttaag tcgatagatt
     4681 tttcccactc tccgaaattc atgcggagat gttcggctgt ttttttattt tttacatcgg
     4741 actgtaattc ttcatattgt ttgccattgt ataaagtgaa aatcagattt tgattgtctt
     4801 tggttgtttg cattttccct ttttcggcaa tggaaacgtt gtcgttattt ttatccagct
     4861 cttcgtaaat agttacttct tcgatggtga tattatcgtc acttttgtcg ccaatcatta
     4921 tagagtagcc atcaatttca ttataaaatt cgttgggttt aatattgagc gcaggttttt
     4981 gtttggtaat atccaccagt gtcaccacaa actttaaata ggaggagggc aagatataat
     5041 tagaaaagaa aaatgcaccg atggcaatta atacaccaat aacaatggtg cttcgcataa
     5101 aggtgaacat ggaaatacct gcacttttaa aagcagccaa ttcaaatcgt tcgcccaaat
     5161 tgccataagt catgatacaa gccaacaaca ccgcaatagg caacgaaagc ggaaccatat
     5221 tggcagaggc ataaaacaat aacttggcaa tgatatacca ttccagtccc tttccaacca
     5281 gatcttcgat atacttccac aaaaaaatca ttaaaaaaat gattaatgcg atagtaaatg
     5341 caatcaggaa gggtcccaaa taggatttta cgatatat
//
LOCUS       DAORKW010000218         5372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_627_length_5372_cov_5.087643, whole genome shotgun sequence.
ACCESSION   DAORKW010000218 DAORKW010000000
VERSION     DAORKW010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_627_length_5372_cov_5.087643"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLB46_09435"
     CDS             <1..901
                     /locus_tag="PLB46_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRF76781.1"
                     /translation="HYETTGPEIWEQTEGKITHLVAGVGTGGTISGIGKYLKEKNPNI
                     KIWGIDTYGSIFKKLKETGTIDKNEIYPYVTEGIGEDFLPENVNIDIIEDFEKVTDKD
                     GAIMTARIVREEGIWVGNSSGAALQGLIQLKDKFIKDDVVVVIFVDHGTRYLGKMFNP
                     DWMMKMGYIDKTGLTAKDLISTNQKGALISVDTHTTVAQALQLMMQNDFSQMPVTDGG
                     RIVGAINESVLYQQIVAHPDIKNKHVGEVMSAAFPFVDISAPIDSISAMLTSDNPALL
                     VRDFKVDKTYIITRHDVMNALTK"
     gene            908..1657
                     /locus_tag="PLB46_09440"
     CDS             908..1657
                     /locus_tag="PLB46_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HRF76782.1"
                     /translation="MSAKRIVSLVPSITELLVDLGLEEHIVGVTKFCVHPEHLRKNTT
                     IVGGTKNLRLEVIRQLQPELILANKEENVREQVEALAAEFKVFTSDIGNFEEALLMIK
                     TVGEITDRLPEAEALITQINQAKSRFEAVNPNNSRPYRTLYLIWQKPWMSVGGDTYIS
                     NMMQIAGFENVCRDRQRYPELSDAAIQSLAPEVILLSSEPFPFTEKHISGLQKLLPEA
                     AILLADGEMFSWYGSRLLQAFDYFTSLRLRI"
     gene            1732..2673
                     /locus_tag="PLB46_09445"
     CDS             1732..2673
                     /locus_tag="PLB46_09445"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRF76783.1"
                     /translation="MVTNDVKLFSGTTSRYLGEKVADYYGQPLGKMTIERFSDGEFQP
                     NILESVRGSYVFFIQSTMFPSDNLMELLLMIDAAKRASASYITAVIPYFGLARQDRKD
                     KPRVSIGSKLVAELLTAAGANRIVTMDLHADQIQGFFDIPVDHLSSTAIYVPYIEENL
                     DLSNVIFASPDVGSTKRNRKYAMHFNTELVICDKYRKKANEIAEMTVIGDVTGKDVIM
                     IDDLVDTGNTLANAANLMKQKGAKTVRAFCTHPVLSGKAYETIENSALEEIVVTDTIP
                     LKRESPKIRVLSSAKLFARAIRNTHEHRSISSLFINN"
     gene            2686..3324
                     /locus_tag="PLB46_09450"
     CDS             2686..3324
                     /locus_tag="PLB46_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRF76784.1"
                     /translation="MQTFVLEGTLRESLGRRASKDARASAMVPCILYGDGENINFEVT
                     KIALKNLVYTPNVYKVLIKVDGKEVESLMREIQFHKVTDEILHIDFLKLNPSKKINHV
                     VPVALTGQSIGVKGGGKLVQRIRKVTIKAFPKDLVDKITVDITDLDVNKTIRVGDIKV
                     ANVEVMGSKSIPIVTVVSPRALKAAAEAAAAADAKAAPVAAAPVAAKEEKKD"
     gene            3429..3986
                     /gene="pth"
                     /locus_tag="PLB46_09455"
     CDS             3429..3986
                     /gene="pth"
                     /locus_tag="PLB46_09455"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRF76785.1"
                     /translation="MKYLIAGLGNFGIDYVNTRHNIGFDVVEKLAADKSVIFSSGRYA
                     DKAEFKFKGKTFILIKPTTYMNLSGKAVKYWMEKENIPLTNLLIITDDLNLPLGKLRI
                     RKNGSDGGHNGLKNINEILQTNQYNRLRFGVGNNFPTGRQVDFVLGKWENTETPMVTE
                     TVTRAAEAVMSIIMEGVDRAMNKFN"
     gene            complement(4013..4915)
                     /locus_tag="PLB46_09460"
     CDS             complement(4013..4915)
                     /locus_tag="PLB46_09460"
                     /inference="COORDINATES: protein motif:HMM:NF020416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF76786.1"
                     /translation="MKKTILLSFLFAAIISSTFAQKSPITPRPMSGNTNGNPKQFEHT
                     EGQFQSINGVNIYFEQYGEGTPVLLIHDNNGSVASFDQQINFLSKKNRVIVADSRGQG
                     SSTLNKDSLTYEQMADDYFMLLQKLELDSVNIVGWGDGGVVALLLAINYPEKVNRIVI
                     GGTAISADTTAFTDTYIAKCNDAIRAAKDSIKEGVLEYKNVLRVLNLRANRLYLDPEL
                     LVNIQAPVLLISGDKDDIKLEHTVALYRALPNAQLSVIPNSNNKVYREAPMVVNNLIN
                     KFFAKNTGNKPGINKAEKQGGGDE"
     gene            5019..>5372
                     /locus_tag="PLB46_09465"
     CDS             5019..>5372
                     /locus_tag="PLB46_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRF76787.1"
                     /translation="MKIFCVGRNYSEHAKELNNEIPEAPVIFMKPPTAILKGKDFYIP
                     EFSSDVHYECELVFRVCKNGKHIEPQFAGKYIDAVTVGIDFTARDVQANQKKKGLPWE
                     IAKAFDNSAVVGEFLP"
BASE COUNT         1676 a          968 c         1117 g         1611 t
ORIGIN      
        1 gcattatgaa actacaggtc cggaaatttg ggaacaaacg gaaggaaaaa ttacgcattt
       61 ggttgcgggt gttggaacag gtggaacgat atcagggatt ggaaaatatt taaaagaaaa
      121 aaatccgaat attaaaattt ggggcatcga tacctatggt tcaattttca aaaaattaaa
      181 ggaaacagga accatcgata aaaatgaaat ttatccttat gtgacggaag gcattggtga
      241 agatttttta ccggaaaatg tgaatatcga tatcatagaa gattttgaaa aagtgacgga
      301 taaagatggt gcaataatga ctgcacgtat tgttcgcgaa gaaggtattt gggttggaaa
      361 cagctcgggt gctgcattac agggtctcat tcaattaaaa gataaattta taaaagacga
      421 tgtcgtggtg gtgatttttg tggatcatgg cacacgttat ttaggcaaaa tgtttaaccc
      481 ggactggatg atgaaaatgg gttatatcga taaaacgggt ttaactgcaa aagatttaat
      541 ttcaacgaat cagaaaggtg ctttaattag tgttgatacg cataccactg ttgcacaagc
      601 attacaactt atgatgcaaa atgatttttc gcaaatgccg gttacggatg gcggacgtat
      661 tgttggtgca attaacgaaa gtgttttata tcagcaaatt gttgcgcatc ctgatattaa
      721 aaataaacat gttggtgaag taatgagcgc tgcatttccg tttgtagata tttcggcacc
      781 aattgattct atcagtgcga tgcttacttc tgataatcct gcattattgg tgcgcgattt
      841 taaggtggac aaaacatata tcattacgcg tcacgatgta atgaatgcat tgactaaata
      901 acttattttg agtgcaaaac gaattgtttc tctggttcca agtattaccg aattgttggt
      961 ggatttggga ctggaagagc atattgttgg ggtgacgaaa ttttgtgtgc acccggagca
     1021 cttgagaaaa aatacgacca tagttggagg gacaaaaaac ctgcgtttag aagtgattcg
     1081 tcagcttcag ccggagctaa ttttggccaa caaagaagaa aatgtccgcg aacaggttga
     1141 agcacttgct gccgagttta aggttttcac cagcgatatt gggaattttg aggaagcact
     1201 tttgatgatt aaaaccgtgg gcgagataac agaccgatta cccgaggctg aagcgcttat
     1261 aacccagata aatcaggcta aaagccgctt tgaagcggtt aatcctaaca attccaggcc
     1321 ttaccgcacc ttatacctca tttggcaaaa accctggatg agtgtgggtg gcgatacgta
     1381 tatcagcaat atgatgcaaa ttgccggttt cgaaaatgtg tgtcgcgacc ggcagcgata
     1441 tccggagtta agtgatgctg ctattcaatc gctggcaccg gaagtaatct tacttagttc
     1501 ggagcctttc ccttttacgg aaaaacatat cagcgggtta caaaaactgc ttcctgaagc
     1561 ggctattttg ttggcggacg gggaaatgtt tagttggtat ggcagcaggc tgttacaggc
     1621 tttcgactac tttacttcgc tcaggttaag aatttgatat ttcattttca ttaaaacttc
     1681 aaaatccgtg tcgtttttgc tatctttgcc gcctcaatta acccgacagc aatggttacc
     1741 aatgatgtaa aactgttttc aggcactacc agccgatacc ttggtgaaaa agtagcagat
     1801 tactacggtc agccccttgg taaaatgacc attgagcggt tcagtgacgg cgagtttcag
     1861 ccgaatattc tggaaagtgt gcggggtagt tatgtgttct ttattcaaag tacgatgttc
     1921 ccgtcggaca acctgatgga gttgttgctg atgattgatg cggctaaaag ggcttctgca
     1981 tcgtatatca ccgcagtaat tccttatttc ggacttgccc gtcaggacag aaaagataaa
     2041 ccacgtgttt caatcggctc gaaactggtt gcggaattat tgactgcagc gggtgccaac
     2101 cgaattgtta ctatggactt acacgctgac cagattcagg ggtttttcga tattccggtt
     2161 gaccacttga gttctacagc gatttatgtt ccttacattg aagaaaatct ggacctttcg
     2221 aatgtgattt ttgcttcgcc tgatgtgggc agcactaaac gcaacagaaa atacgccatg
     2281 cattttaaca ccgaactcgt tatttgcgat aaataccgca aaaaagcgaa tgagattgct
     2341 gaaatgactg taattggtga tgttacaggt aaagatgtga ttatgatcga cgacttggtt
     2401 gacaccggta atacgcttgc caacgcagcc aatttgatga aacaaaaagg cgcaaaaacg
     2461 gtgcgtgcgt tttgtaccca tcctgtatta agcgggaagg cttatgaaac aatcgaaaat
     2521 tctgctttag aggaaattgt tgttaccgac actattccgc tgaaacgtga atcaccaaaa
     2581 attcgtgtgt tgtcatctgc gaaattgttt gcaagagcaa ttcgtaatac gcacgaacac
     2641 agaagtatta gttcactatt tattaataat taaatcaaaa ttcaaatgca aacttttgta
     2701 ttagagggaa cattgagaga atcacttggc cgccgcgctt cgaaagatgc acgtgcttca
     2761 gctatggttc catgtatcct gtatggcgat ggcgaaaaca ttaatttcga agtaaccaaa
     2821 attgcactca aaaacctggt gtacacacca aacgtttaca aagtgcttat taaagttgat
     2881 ggtaaagaag ttgaatcgtt aatgcgtgaa attcagtttc acaaagttac cgatgaaatt
     2941 ttacacatcg atttcttaaa attaaatcca tctaaaaaaa tcaatcacgt tgtgccagtt
     3001 gctttaaccg gacaaagtat tggtgtaaaa ggtggtggta aacttgttca acgtatcaga
     3061 aaagtaacta ttaaagcttt tcctaaagat ttagttgaca aaattacagt tgacattact
     3121 gaccttgacg ttaacaaaac aattcgtgtt ggtgatatta aagttgctaa tgttgaagta
     3181 atgggatcta aaagtattcc aattgttact gttgtttctc ctcgtgcatt aaaagctgct
     3241 gctgaggcag ctgctgctgc cgatgctaaa gctgctcctg tggctgctgc acctgttgct
     3301 gcaaaagaag agaagaaaga ttaataaacc tttttcttta aacgaagcaa tttctatctt
     3361 taaatatgaa attgcttcgt tttttatttc caaaaaagca tacgcaaact gtttcaactg
     3421 aacctgaaat gaaatattta atcgccggtt taggaaattt tggaattgat tatgtcaata
     3481 cacgacacaa catcggtttt gatgtcgtgg aaaaattagc cgctgataaa tctgttatat
     3541 tttcttccgg ccgttatgcc gataaagcgg aatttaaatt taaaggcaaa acatttattt
     3601 taattaaacc tactacttac atgaatttaa gtggtaaggc agtgaaatat tggatggaaa
     3661 aagaaaatat tccattaacc aatctcctca taataaccga tgatttgaat ttaccactcg
     3721 gcaaattacg cattcggaaa aatggaagcg atggcggtca taacggttta aaaaatatca
     3781 acgaaatatt acaaactaat cagtacaacc gtttacgttt tggtgtaggt aataacttcc
     3841 ctaccggcag gcaagtggac tttgtattgg gcaaatggga aaacactgaa acacccatgg
     3901 taactgaaac cgtgacacgt gcggctgaag ctgtaatgtc aataattatg gaaggcgttg
     3961 accgcgcaat gaataaattt aattaagcaa aaacaggaaa tagtaacgat tattattcgt
     4021 cgccaccacc ttgtttttct gccttattaa ttcccggttt attgccggta tttttagcaa
     4081 aaaacttatt gattaaattg ttcaccacca ttggtgcttc gcgatatact ttgttattac
     4141 tattcggaat tacacttaat tgtgcattcg gcaacgcgcg atataacgca acagtatgtt
     4201 ctaatttaat atcatcttta tctccgctaa ttaataaaac cggcgcctga atatttacta
     4261 ataattccgg gtcaagataa aggcggttag cacgtaaatt taatacgcgt aaaacatttt
     4321 tatattccaa aaccccttct ttaatagaat cttttgctgc gcgaatggca tcgttacatt
     4381 ttgcaatata ggtatcggta aatgcagttg tatcagcact aattgctgtt ccaccaataa
     4441 caattctgtt tactttttcg ggataattaa tcgctaacaa taatgccact acaccaccat
     4501 cgccccagcc aacaatatta acgctatcga gttctaattt ttggagtaac ataaaatagt
     4561 catcagccat ttgttcatag gttaaactat ctttatttaa agttgaactc ccctgccctc
     4621 tgctatctgc aactattaca cgattttttt tcgataaaaa atttatttgc tggtcaaatg
     4681 atgcaacaga accattatta tcgtgaatta ataaaacagg tgtgccttca ccatattgtt
     4741 cgaaataaat attaacacca ttaatagatt gaaattgtcc ttcagtatgt tcaaattgtt
     4801 taggattacc attggtattg cccgacattg gtcgcggggt aattggcgat ttttgtgcga
     4861 atgttgacga aataatcgca gcaaataaaa aactgagtaa aattgttttt ttcatatcga
     4921 attaattcag atttaaagat accatattaa atcaaaacag ctatttgagc ccatttcgct
     4981 gcaattttaa tgccctttcg taactttgta cacgaattat gaaaatattt tgtgtaggtc
     5041 gcaactacag cgaacatgct aaagagctca acaacgagat tccagaagcc ccggttattt
     5101 ttatgaagcc tccaacggcg attttaaaag gtaaagactt ttatatccct gagttttcgt
     5161 cggatgtgca ttacgaatgc gaattggtat tccgggtttg caaaaacggg aaacatatcg
     5221 agcctcagtt tgcaggtaaa tatattgatg ccgttaccgt tgggatcgac tttactgccc
     5281 gcgacgtgca ggcgaatcag aagaaaaagg gccttccctg ggaaattgcc aaagctttcg
     5341 acaactcggc agtggttggg gagtttttgc ct
//
LOCUS       DAORKW010000219         5369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_628_length_5369_cov_4.493414, whole genome shotgun sequence.
ACCESSION   DAORKW010000219 DAORKW010000000
VERSION     DAORKW010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_628_length_5369_cov_4.493414"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLB46_09470"
     CDS             <1..649
                     /locus_tag="PLB46_09470"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HRF76788.1"
                     /translation="KAVILYFPIQPMETIEVGPFEFHDKIPLKKNLKAAGVRVVPHGL
                     IRHGAFVAKGVIVMPGYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVL
                     EPVQAAPVIIEDNCFIGSRCIVVEGVIVEEEAVLGANVVLTQSTKIIDVTGPEPITYV
                     GRVPKRSVVIPGAYTKQFPAGEYQVPCALIIGTRKESTDKKTSLNDALRENAVAV"
     gene            670..2106
                     /locus_tag="PLB46_09475"
     CDS             670..2106
                     /locus_tag="PLB46_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRF76789.1"
                     /translation="MTKKTITQFTFSDEEKYLFNTIANCATELGYPCYIVGGYVRDKI
                     LQRSYKKDIDFVCVGSGITLARKVAAALPGNPFVAEFKNFGTAHFKYHDIDVEFVGAR
                     KESYVKESRNPIVENGTLADDQLRRDFTINAMAISLNAEDFGALIDPFDGLTDLEDRI
                     IRTPQNPEITFSDDPLRMMRAIRFASQLNFDIESTTYEGIKSAKERIKIISQERITDE
                     LNKILLSPKPSIGLYYLSDSGLLEYIFPELEAMHGTDMIDGNGHKDNFYHTLEVVDNI
                     SATTDNLWLRWAALLHDIAKPLTKKYIPPTGWTFHGHEIVGAKLAKRIFTRMKLPLQQ
                     ELKQVQNLVSLHMRPISLTKENVTDSAIRRLLFEAGEDLEGLMLLCQADITTKNRLKI
                     KRYKENFEMVREKLKDVEERDHIRNFQPPITGELIMATFNIKPGKEVGLIKDYVRESI
                     LDGLIPNEYDKAYDLMVAKGKELGLTPQ"
     gene            2156..3046
                     /gene="miaA"
                     /locus_tag="PLB46_09480"
     CDS             2156..3046
                     /gene="miaA"
                     /locus_tag="PLB46_09480"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRF76790.1"
                     /translation="MSNKTLITIAGPTASGKTALAIELAKKWNTVIISADARQFYTEM
                     SIGTAKPSPEELAAVPHYFINNLSVQQPYDVGKYADEVLELLDTLFQQHNNIILCGGS
                     GLFIKAVTDGLDDLPETLPEIREQVQLAYEQNGLLWLQQTIQSKDPDYFQVVDINNPK
                     RLLRALEVITQTGNTLSSYHKKVQQPRYFSNIKICLDLDRKNLYERINERVNIMIKNG
                     LADEVKTLIPFRNLNALQTVGYTEMFEYFDSKITLQRAIELIQQHTRNYAKRQLTWFR
                     RDPSFIWCKPELSEIEKHCF"
     gene            3065..3571
                     /locus_tag="PLB46_09485"
     CDS             3065..3571
                     /locus_tag="PLB46_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRF76791.1"
                     /translation="MKILLLSDTHGYLDDAIKRHANEADEIWHAGDFGTIALADELQK
                     IKPLRGVYGNIDGKDVRLSYPLDNTFQMDGIKVFMTHIGGYPGRYEPRLKPIISQHPP
                     HLFICGHSHLLKVIRDPKNNMLCMNPGAAGIHGFHKVRTMLRFVICDGKINNLEAIEL
                     GLRGKMHQ"
     gene            3735..4172
                     /locus_tag="PLB46_09490"
     CDS             3735..4172
                     /locus_tag="PLB46_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRF76792.1"
                     /translation="MLTVRRRPNGVPAFVNVFEDFFNQAFPEKYVSEKGEFIPSVNIA
                     ENEKAFTLEFAAPGFEKTDFDVNIEKNFITISAKKEVKDEVKAKNYTRKEFVFSSFKR
                     TFNLPETIEISGISATYENGILLVELPKKEVKAETAATKIAIK"
     gene            complement(4258..5169)
                     /locus_tag="PLB46_09495"
     CDS             complement(4258..5169)
                     /locus_tag="PLB46_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76793.1"
                     /translation="MKIRLLSAITFTTLLTAAYGQVPNGNLEAWTTYTSAAFVDVDVP
                     DGWDVPDKIAADLGITDRNCTKETVNTHGGSAAARLETKTLNVLGTALDVPGTITTGI
                     ISFDFGTFTPSVEGGAPVTAAYESLAGYYQYAPAGGDTMNVVVLMLLGEDTIGMGQYR
                     DDLTSTSYQMFECPITYFVAAVPDQMQITITSSGGFSSLVPGSVLYVDDLSVTGGINV
                     EDFEGYGISRNIYPNPASDYININNPAHNDVTMEVYNISGQRVDVVNLAPEMNSVNLE
                     AYASGIYSFRLIDNGTEVYSNKFVVRK"
BASE COUNT         1799 a          914 c         1057 g         1599 t
ORIGIN      
        1 aaaagcggtg attttatatt ttcctattca accaatggaa accattgaag ttggcccatt
       61 cgaatttcat gataaaattc cgctgaaaaa aaaccttaaa gcggccggag ttcgtgttgt
      121 gccgcatggt ttgattcgac atggcgcctt tgttgccaaa ggagtaattg taatgccggg
      181 ttatgtaaat attggtgctt atgtagatga aggcactatg gtagatacct gggcaacagt
      241 tggtagttgt gcgcaaattg gtaaaaatgt gcacctgagt ggtggtgttg gtattggcgg
      301 tgtattggaa cctgttcagg ctgctccggt aattattgag gataattgtt ttattggctc
      361 aagatgtata gttgtagaag gtgttattgt tgaagaagaa gcggtattgg gtgctaatgt
      421 tgtgctcact caatcgacaa aaattattga tgtaaccgga cctgaaccaa tcacctatgt
      481 tggtcgcgtt ccgaaacgta gtgtagtaat tccgggtgct tataccaaac aatttcctgc
      541 aggtgaatat caagtgccct gtgcattaat tattggcaca agaaaagaaa gcaccgataa
      601 aaaaacaagt ttaaatgacg cattgcgcga aaacgctgta gcggtataag aaaattaaat
      661 aaagtaagcg tgacaaaaaa aaccataact caattcactt tttctgatga agaaaaatat
      721 ttgttcaata ccattgcaaa ttgtgcaaca gaattgggtt atccatgtta tatagttggg
      781 ggttacgtac gtgataaaat tttacaacgc agctataaaa aagatattga ttttgtttgt
      841 gttggaagtg gaattacatt agccagaaaa gttgcagctg ctttaccggg caatcccttt
      901 gttgctgagt ttaaaaattt cgggaccgca catttcaaat atcatgatat tgatgttgaa
      961 tttgttggtg caagaaagga atcttacgta aaagaaagtc gtaaccctat tgttgaaaat
     1021 ggtacacttg ccgatgatca attgcgaagg gactttacga ttaatgcaat ggctatttcg
     1081 ctgaatgcag aagactttgg tgcattaatt gacccatttg acggattaac tgatttggag
     1141 gacaggatta ttcgtactcc acaaaatccg gaaattactt ttagtgatga cccgcttcga
     1201 atgatgcgcg ccattcgttt tgcatcacaa ttaaattttg atattgaaag tacaacctac
     1261 gaaggaatta agtcggctaa ggaacgtatt aaaatcatat cgcaggaaag aattaccgac
     1321 gaactgaata aaatattact atcgccaaaa ccatccatcg gattatatta tttatctgat
     1381 tccggtttat tggaatatat tttcccggaa ctcgaagcca tgcatggtac agatatgatt
     1441 gatggcaatg gacataaaga taatttttat catacgctgg aagtagtaga taatattagt
     1501 gcaactaccg ataatttatg gttgcgttgg gctgcattat tacacgatat tgccaaacca
     1561 cttaccaaaa aatatatacc accaacagga tggacatttc acggacatga aattgtaggc
     1621 gctaagctgg ctaaacgcat ttttaccaga atgaaactac ccttgcaaca agaattaaaa
     1681 caagtgcaaa atctggtgag tttacacatg cggccaattt cactgactaa agagaatgta
     1741 accgattcgg ctatccgaag attattgttt gaagcaggtg aggatttaga ggggttgatg
     1801 ttattgtgtc aggcagatat tactactaaa aatcgcctga aaattaaacg gtataaagaa
     1861 aattttgaga tggtacgcga aaaattaaag gatgtggaag aacgcgacca tatccgtaat
     1921 tttcaaccac ctattaccgg tgaattaatt atggctacat ttaatattaa accgggaaaa
     1981 gaagtaggtt taattaaaga ttatgtccgc gaatctattt tagacggatt aattccgaac
     2041 gaatacgata aagcatatga tttaatggta gcaaaaggta aagaattggg attaacacct
     2101 caataatatt acgattttct tccgtgttag ttacagaaaa ataaacatgc ctcaattgtc
     2161 aaataaaact ttaattacca ttgccggacc aacagcttcc ggtaaaacgg cattggcaat
     2221 tgaattggcg aaaaaatgga atacggtaat tatatccgca gatgcgcgtc agttttatac
     2281 tgaaatgagt ataggtactg ctaaacccag ccctgaagag ctcgctgctg tgcctcacta
     2341 ttttattaat aatttatctg ttcaacaacc ttacgatgtt ggcaaatatg ctgatgaagt
     2401 tttagaattg ctggacacac tttttcagca acacaacaat ataattttat gcggaggctc
     2461 cggattattt attaaagccg ttactgatgg attagatgat ttaccggaaa cattgcctga
     2521 aatccgcgaa caggtgcagc ttgcatatga gcaaaatgga ttgttgtggt tgcagcaaac
     2581 cattcaatca aaagaccctg attattttca agttgttgat attaataatc caaaacgatt
     2641 gttgcgagca ttagaagtaa ttacacaaac aggaaatacc ttatcatcgt atcataaaaa
     2701 agtgcaacaa ccgcgatatt tttccaatat taaaatttgc ctcgatttag acaggaaaaa
     2761 tttatatgag cggattaatg aacgggtaaa tattatgata aagaacggat tagcggatga
     2821 ggtaaaaact ttaattccgt ttcgaaattt aaatgcatta caaactgttg gatataccga
     2881 aatgtttgaa tattttgata gcaaaataac attacaacgt gccattgaac ttattcaaca
     2941 acacacacgc aattatgcca aacgacaatt aacctggttc aggcgtgacc catcatttat
     3001 ttggtgtaag ccggaattgt cggaaataga aaaacattgt ttttgaattt tattatttgc
     3061 ctgcatgaaa atattattac tttctgatac ccatggttat ttagatgatg ccattaaaag
     3121 acatgcaaac gaagccgatg aaatttggca tgccggagat tttggaacaa tagcacttgc
     3181 tgatgagtta caaaaaataa aacctttacg tggtgtctat ggcaatatag atggcaaaga
     3241 tgtaaggctc tcctaccctt tagataatac ctttcaaatg gatggtatta aagtttttat
     3301 gacgcatata ggaggatatc ccggccgtta cgaacctcgc ttaaaaccaa ttattagtca
     3361 acacccgcct cacctcttta tttgcgggca ttctcatttg ctaaaagtaa ttcgcgaccc
     3421 gaaaaacaat atgttatgta tgaaccccgg agctgccggt atacatggat ttcataaagt
     3481 gcgcaccatg ttaaggtttg taatttgtga tggcaaaata aataatctcg aagcaattga
     3541 gttgggactt cgcggcaaaa tgcaccaata agccattttg tcagtatttt ttatttgaat
     3601 acgtcatttt gtcattgcaa aatattgatt tttgcaacct ttggcataaa aaatctgcaa
     3661 aattggtctt ggtatttagt ttgaataaat gctttcgtca aacaaatttt caaactaaaa
     3721 aacaaaatat gactatgtta actgtaagac gcagaccaaa cggagttccg gcatttgtaa
     3781 atgtatttga ggacttcttt aatcaagctt ttcctgaaaa gtatgtaagt gaaaaaggtg
     3841 aatttattcc ttcggtaaat attgccgaaa atgaaaaagc attcacgtta gagtttgcag
     3901 ctccgggatt tgaaaaaacg gattttgatg taaacatcga aaaaaacttc atcactatta
     3961 gcgcaaaaaa agaagtgaaa gatgaagtta aagcaaaaaa ttacaccaga aaagaatttg
     4021 tattcagcag cttcaaacgc acattcaatt tacctgaaac cattgaaata tcaggtattt
     4081 cagcgacgta tgaaaatggt attttattag tagaattacc aaaaaaagaa gtaaaagcag
     4141 aaactgctgc cacaaaaatt gcaataaagt aaagggtaat agggttgatt tcataaaaaa
     4201 gaaaaccccg acaatattgt cggggttttg cttttagatg ctggttaagc aacggaatta
     4261 ttttctaaca acgaatttgt tactgtaaac ttcagttccg ttatcgatta aacggaaaga
     4321 atagatacca cttgcataag cttcaagatt aacactattc atttctggtg caaggttaac
     4381 aacgtcaact ctttgaccac tgatgttgta aacttccata gttacatcat tgtgagcagg
     4441 gttgttgata ttgatataat cacttgcagg gttaggataa atgttacgag aaataccata
     4501 accttcaaaa tcttcaacat tgataccacc tgttactgat aaatcgtcaa catacaatac
     4561 tgaaccaggc actaatgaac taaatccacc tgatgaagta attgtaattt gcatttgatc
     4621 aggaactgcc gctacgaaat aagtaatagg gcattcgaac atttggtagc tggttgaagt
     4681 taaatcgtca cggtattgac ccattccgat agtatcttca cctaataaca ttaatacaac
     4741 cacattcatg gtatcgccac ctgcaggagc atattggtag taaccggcaa gtgattcgta
     4801 agcagcggta accggagcac caccttcaac tgaaggggta aaagtaccga agtcaaagct
     4861 gataatacct gtagttattg ttcccggaac atctaatgcc gtccctaaaa cgtttaatgt
     4921 ttttgtttct aaacgagctg ctgctgaacc accatgcgtg ttaacagttt cttttgtaca
     4981 gtttctgtct gtaataccta agtcagctgc aattttatca ggcacatccc aaccgtccgg
     5041 tacatctaca tcaacaaaag ctgctgaagt gtaagttgtc caagcttcga ggttgccatt
     5101 cggaacttgt ccgtatgcgg ctgttaaaag ggtggtaaat gtaatagcac taagtagacg
     5161 aattttcata ctcttttttt tatttgttat tgaatgaatt tgattgttta caaaggtaaa
     5221 tataggatta tttggtgaaa ttttaaattt tcgttaatta ttcaatcgtt aattaactta
     5281 taacctagcg ctccaagtgc agcgccgaca agcgaagtag ccagatggac agcaatgtcg
     5341 agcccgtgca caggcaagtc aaatatatt
//
LOCUS       DAORKW010000220         5304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_643_length_5304_cov_4.096209, whole genome shotgun sequence.
ACCESSION   DAORKW010000220 DAORKW010000000
VERSION     DAORKW010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_643_length_5304_cov_4.096209"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="PLB46_09500"
     CDS             <1..1731
                     /locus_tag="PLB46_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76794.1"
                     /translation="VGFITLLQTADGGYILGIDSWSGNTGMKTDTLSGASDFWIIKID
                     ASGNVEWQKAYGSTGADILTFLFITNDGGYFFGGHSDSNSGGAKSENNFGFNDYWVVK
                     IDASGNVIWDNTVGGTDEDQLWNATQTADGGFVLVGASVSGISGEKTEPHVGPYTYTD
                     YWIIKLNNSGTIQWQNTIGGNRIDAALAVFENATGELFINGNSGSIANGDKTEPNYGA
                     ANTADYWILKLNATGSIIWQKSIGGSNSDLATATTLCSDGGLIIAGTSFSPASGNKTT
                     NLVGGDDIWIVKLEGGCAFNVELCNELDDNCNGLIDDGAVNTISIAALSGTVFCQGGS
                     AVIEATHSGTGLQWRRNGVNIAGATSSTYTATTSGAYTCFSYSTCDTTESTAVNVTVN
                     KNPKAQITAGGPTTFCTGGSVVLTEAAVAGCTYQWYKGVTPIAGATSLTYTATTSGNY
                     KCRVTKTATGCFKNSNAIAVSVPCKEGLPAGEEGLPDSEAGEMSNGEDAFSIFPNPNH
                     GSFNISGSISSETQIEIFNSLGQLIYTKKVNSLNHFQAAISINNFVTGIYYVRLTNEF
                     TITEQKMIIE"
     gene            1832..>5304
                     /locus_tag="PLB46_09505"
     CDS             1832..>5304
                     /locus_tag="PLB46_09505"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF76795.1"
                     /translation="MRQSIIIILIILITELKAQEPVIAWQQTIGGSGLDYFKSCNQTS
                     DNGYIIGGYSYSGISGDKTEGNIGSADYWVLKLDSVGDIEWQNTIGGASTDFLASVEQ
                     TFDGGYIIGGYSKSGASGDKTELNFIGGEGYDYWIVKLNALGSIEWQNTIGGNNDDFL
                     TSIHQTMDGGYIIGGYSASTLSGDKSEGNMGGAGTKDYWILKLNSTGGIIWQNTIGAD
                     GNDVLAEIRETAEGNYIVGGYSDSKKNGDKTIKRWGSLSDYWVMQLNSSGTIMWQNVF
                     GGLDSDLLTSVIQLADGGFLFGGYSDSDITGNKSKHLYVGSHTDYWLVKTDALGNIIW
                     DKVLGGSENEIITSMTETAGQNLLIAGYSISPSSFDKLEPTQGLEDYWILELDNSGKT
                     LWENDLGGILNERPYAIGNTQDGGFFVLGYSASLISGDKTEVGSGSIDGWMLKFNPSN
                     CISGPYYFDFDMDNAGDVTTAFNACELTYRYVENFADCNPLHSNQNPLAPEVCDGIDN
                     NCDGLIDEGIFGCNPGPDVLWQNTIGGVESDNIADIHPTSDGGYILIAGSDSDISGDK
                     NANSKGAIDYWIVKLDAIGNITWQKTIGGSGNDWPKCISQTTDGGYIAGGYSSSGISG
                     DKNEASLGGDDFWIIKLDAFGNIEWQNTIGGNSTDLLNDISQTFDGGFIVGGSSFSGI
                     SGDKTTPNATNDGWILKLNATGVIEWQKSIRGNLFDILDNIKQTTDGGYIAGLYSESG
                     IGLDKTAPSQGAYDYWIVKLDAAGNIIWQNTIGGGGGDYLYAVSQLSDGSYIIGGTSF
                     SSASGNKTEVLIGGSDLWIVKLDISGNLVWQNTIGGADLDGLNAIRATQDNGFILGGF
                     SWSDISGDKVENKIIGGFEDAWIMKLNSEGEIVWQNAIGGNNNDICINIEQCFDGTFI
                     AGVASASELSGDKNENTPANVDSWIIKFDIDCVPVAEICNSIDDNCNGNIDENIAETI
                     SISAGGPTTFCQGGNVLLTAIYSGATVQWKKNGTNIPGATSPTYLVTTKGTYTCETTS
                     LCGTATAVGIFVNVNKNPPATITAGGPTNFCAGGSVILTANTGGGLSYQWYKGATPIA
                     GATSLTYTATTSDNYKCRVTKTATGCFKNSNPINVAVQCKEGEEVLLKTTLNIQPNPN
                     NGTFTISAHSLS"
BASE COUNT         1720 a          838 c         1165 g         1581 t
ORIGIN      
        1 gttgggttta ttacccttct gcaaactgcc gatggcggtt atattttagg catcgattcg
       61 tggtcgggaa ataccggaat gaaaaccgat acactttccg gtgcttcaga tttttggatt
      121 attaaaattg acgcttccgg taatgtggaa tggcaaaaag catacggcag cacaggcgca
      181 gatattctta catttttatt tattacgaat gatggtggtt atttttttgg aggacattca
      241 gactccaatt caggtggtgc gaaatcagaa aataattttg gatttaatga ttattgggta
      301 gtaaaaattg atgcctccgg aaatgtgata tgggataata ctgttggtgg taccgatgaa
      361 gaccaacttt ggaatgccac gcaaaccgca gatggtggtt ttgtattggt aggcgcttct
      421 gtatccggta tttcaggtga aaaaacagaa cctcatgttg gaccatatac ctataccgac
      481 tactggatta ttaaattaaa taatagcgga actattcaat ggcaaaatac aataggtggc
      541 aacagaattg atgctgcctt agcagttttt gaaaatgcaa caggcgaatt attcatcaat
      601 ggcaattcag gttctattgc gaacggcgat aaaacagaac ctaattacgg tgcagcaaat
      661 actgccgatt attggatatt aaaattaaat gctaccggtt caattatttg gcaaaaatca
      721 attggtggaa gtaattccga tctcgcgaca gccacaacac tttgcagcga tggtggactt
      781 attattgcag gaacatcctt ttcaccggca tcaggaaata aaacaaccaa tcttgttggt
      841 ggcgatgata tctggatagt gaaactggaa ggtggttgcg catttaatgt tgagttgtgc
      901 aacgaattag atgataattg taacggttta atagatgatg gcgcagtaaa tacgatttcc
      961 attgctgctt tatcaggaac tgtattttgt cagggaggaa gtgctgtaat tgaagctacc
     1021 catagtggaa ctggattgca atggagaaga aatggtgtaa atattgccgg tgctacttca
     1081 tctacttata ctgccaccac atccggtgct tatacttgct ttagttatag cacttgcgat
     1141 acaaccgaat caactgccgt aaatgtaaca gtaaataaaa atccaaaagc gcaaataact
     1201 gcaggagggc caacaacatt ttgcaccggt ggcagtgttg tattaacaga agccgcagtt
     1261 gcaggttgca cttaccaatg gtataaaggc gtcacaccaa ttgcaggcgc aacttcgtta
     1321 acgtatactg caactacatc cggtaattat aaatgccgtg taaccaaaac agcaacagga
     1381 tgttttaaaa attcgaatgc aattgcagtg agtgtgcctt gtaaagaagg tttacctgca
     1441 ggcgaggaag gcttgcctga cagtgaggca ggggagatgt cgaatggcga agatgcattt
     1501 tctattttcc caaatccgaa tcatggcagc tttaatattt ctggttcgat ttcaagtgaa
     1561 actcaaattg aaatttttaa ttcacttggt caattaatat ataccaaaaa agttaattca
     1621 ttaaatcact tccaggcagc tatttcgatt aacaattttg ttaccggtat ttattatgtg
     1681 cgattgacaa atgaatttac gattactgag caaaaaatga ttattgagta attacttgat
     1741 ataaattgaa ttacataaag aacagctcaa ataaataatc ccaatgaaaa tacgaaagca
     1801 atttttgaaa agtagtatta aattcttagt gatgaggcaa agtataatta tcattttaat
     1861 tatattaata acagaattaa aagctcaaga gcctgttata gcgtggcaac aaactattgg
     1921 tggttcgggt cttgattatt ttaaatcttg caatcagact tccgataatg gatatattat
     1981 tggcggctat tcctattccg gaatttcggg tgataaaact gaaggtaata tcggctcagc
     2041 agattactgg gtattaaaac ttgattcggt tggcgatatc gaatggcaaa atacaattgg
     2101 tggcgcatcg acagattttc ttgcatcagt tgagcaaact tttgatggtg gttatattat
     2161 tggtggatat tccaaatcag gggcatccgg agataaaacg gaattaaatt tcattggagg
     2221 agaaggttac gattattgga tagttaaact gaacgccctt ggaagtattg aatggcaaaa
     2281 taccattggt ggcaacaatg atgatttcct aacaagtata catcaaacaa tggatggcgg
     2341 atatattatc ggtggttatt ccgcatcaac attatccggc gataaatcgg aaggaaatat
     2401 gggcggagcc ggcacaaaag attattggat attgaaatta aattcaacag gtggtatcat
     2461 ttggcaaaat acaattggcg cagacggaaa tgatgttctt gcggaaatca gagaaactgc
     2521 tgagggtaat tatattgttg gcggttattc agattcaaaa aagaatggtg ataaaacaat
     2581 aaaacgatgg ggttctttaa gcgattattg ggttatgcaa ttaaacagct caggaactat
     2641 tatgtggcaa aatgtattcg gaggattaga tagtgatctg cttacttccg ttattcaatt
     2701 agctgatggt ggttttttgt tcggtggata ttctgattca gatattactg gaaataaatc
     2761 aaaacatctt tatgtaggtt ctcataccga ttattggtta gtaaaaaccg acgctttagg
     2821 aaatataatt tgggataaag ttttaggtgg aagcgaaaac gaaatcatca cctcaatgac
     2881 agaaacggca gggcaaaatt tattaattgc aggatattct atttctccat cttcattcga
     2941 taaacttgag cctacacaag gtctggaaga ttattggatt cttgaattag ataattctgg
     3001 aaaaacgttg tgggaaaatg accttggtgg tatactcaat gagcggccat atgcaattgg
     3061 aaatacgcag gatgggggat tttttgtact aggatattct gcttctctga tatccggcga
     3121 taaaacagaa gttggctcgg gaagtattga tggctggatg ttaaaattta atccatcgaa
     3181 ttgcatctca ggtccttatt atttcgattt tgatatggac aacgcaggtg acgttactac
     3241 tgcttttaat gcatgtgaat taacttatcg ttatgtagaa aatttcgctg attgtaatcc
     3301 tttgcattcc aaccaaaatc ctttagcccc tgaagtttgt gatggaatag ataacaattg
     3361 tgatgggctt attgatgaag gaatttttgg atgtaatccc ggccctgatg ttttatggca
     3421 aaatacaata ggtggagttg aatctgataa tattgcagac attcatccca catcagacgg
     3481 tggatatatt ttaattgcag gatcggattc cgatatttcg ggtgataaaa atgcaaattc
     3541 aaaaggcgct atcgattact ggattgttaa acttgatgct attggtaata ttacctggca
     3601 aaaaactatt ggaggttcag gaaatgattg gccaaagtgt attagtcaaa ctacagatgg
     3661 cggttatatt gcaggaggat attctagttc cggaatttca ggagacaaaa atgaggcaag
     3721 tttgggaggt gatgattttt ggattattaa attggatgcg tttggaaata ttgaatggca
     3781 aaatactatt ggagggaatt ctactgattt attaaatgat ataagccaaa cttttgacgg
     3841 aggatttatt gtagggggaa gttcattttc cggtatttca ggtgataaaa ctacacccaa
     3901 cgcaacaaat gatggatgga tattaaaatt aaatgcaaca ggagtaattg aatggcaaaa
     3961 atcaattaga ggaaatttgt ttgatatttt ggataatatt aaacaaacaa ctgatggcgg
     4021 atatattgca ggattgtatt ctgaatcagg cattggatta gataaaactg caccatctca
     4081 aggcgcttat gattattgga ttgtaaaact tgacgcagcc ggaaatatca tttggcaaaa
     4141 tacaattgga ggggggggcg gcgattatct ttatgctgta agccagttaa gtgatggcag
     4201 ttatattata ggtggaacgt cattttcatc tgcatctggt aacaaaactg aagtacttat
     4261 tggtggtagc gatttgtgga ttgtaaaatt agatatatcc ggaaatctgg tttggcaaaa
     4321 cactattgga ggtgcggatt tagacggatt gaatgctatt cgtgctacgc aggataatgg
     4381 gtttattcta ggcggatttt cttggtcgga tatatcagga gataaagttg aaaataaaat
     4441 aattgggggt tttgaagacg cctggatcat gaaattaaat tcagaaggag aaattgtttg
     4501 gcagaatgct attggtggta ataacaacga tatttgtata aatatcgaac aatgttttga
     4561 tgggacattt atagcgggag ttgctagcgc atctgaactc tccggcgata agaatgaaaa
     4621 tactccggca aatgttgata gctggataat taaatttgat attgattgtg tccctgtagc
     4681 tgaaatatgc aattccattg acgataattg taacggaaac atcgacgaaa acatcgccga
     4741 aacaatttct atctccgcag gaggaccaac aactttttgt caaggtggaa acgtattatt
     4801 aaccgcaatt tattccggtg caacagtaca atggaaaaag aacggtacaa acattcccgg
     4861 tgctacatca ccaacctatt tagttacaac aaaaggaact tacacttgcg aaacaaccag
     4921 cctttgcgga actgcaactg ctgtaggaat atttgtaaat gtcaataaaa atcctcctgc
     4981 aacaattact gcaggtggac caacaaactt ttgcgcaggt ggttctgtaa tattaacggc
     5041 aaatacaggc ggtggattga gttaccaatg gtataaaggc gcaacaccaa ttgcaggtgc
     5101 tacatcatta acatatactg caactacctc agataattat aaatgcagag ttacaaaaac
     5161 tgcaacggga tgttttaaaa attcaaaccc aattaatgtt gctgtacaat gtaaagaagg
     5221 tgaagaagtg ctgcttaaaa ctacccttaa catccaaccc aaccccaaca acggcacatt
     5281 caccatctcc gctcactcac tttc
//
LOCUS       DAORKW010000221         5300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_647_length_5300_cov_5.078742, whole genome shotgun sequence.
ACCESSION   DAORKW010000221 DAORKW010000000
VERSION     DAORKW010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_647_length_5300_cov_5.078742"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLB46_09510"
     CDS             complement(<1..464)
                     /locus_tag="PLB46_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76796.1"
                     /translation="MNRIYIIFTILFISAFGQLFAQDTTIYRQANASLFSNNYTFIKK
                     SKSDKFGTFIQCSRTDDMQYWYGEGKFTETNKKYFLTFDTTNYYNRIETIASTSHSDT
                     LYIKWFDWRGEQQEWFSIRFQDTINNKNIYHTGFFEYFGHTAPPIPDQCDPL"
     gene            complement(570..2195)
                     /locus_tag="PLB46_09515"
     CDS             complement(570..2195)
                     /locus_tag="PLB46_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRF76797.1"
                     /translation="MFGAKTLAVIFMDGYKAKKIEETLKGKDFLGYEIINLINNGKSA
                     AVFKAKDNSGNEFAIKIFDNELIERFGHEIQESRIEKEISLKGHTILNLIKIIDGGKS
                     QIDADEYYYIIMEYIDGLNLKEFIIEKNYDNTIIQKVVQTIFTITEELLLKGIVHRDI
                     KPENIMISKANEIILMDLGVLKLIGVPSQSDQDEKQFVGTLRYAPPEFLLRTEEDSLN
                     GWKAVNLYQIGGLMHDLITKKELFHDYNPYSKLVLAIKEDAPIISNETLPFSTIQLSR
                     DLLIKDWNKRLAVNSNKRILDFCSFNPDGISSIDKEFEDILSMTSSHKTKFEEIEKIT
                     RSNQEKNERKKQISESIDKIIDECFQKIKTIGLIHRIEKSKLFFFDTDREMRQKQAVK
                     NYVYKLESDISKGFPRPMFILVKFSNDENSFAEISLLAMFLSVFVKINMDEPPLIFKE
                     IGKELTPYHQRNQQTFFNFKTYQAFNGTVGFDEQFKNNITLELIKLIKVALKGVETEV
                     KAELEQRENFAKGEQRTTVTYGTPKTKLFHSLE"
     gene            complement(2199..3431)
                     /locus_tag="PLB46_09520"
     CDS             complement(2199..3431)
                     /locus_tag="PLB46_09520"
                     /inference="COORDINATES: protein motif:HMM:NF025448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4263 domain-containing protein"
                     /protein_id="HRF76798.1"
                     /translation="MKEEPTEYDYFTNKRTDRVYLSRALDEKFLSKNEKGDLEEKIRP
                     FRIVSKIIENIEDHKFIKDGKQIALRITPGGRQEIKAKFYEDTRGITTLTIQKFTTDT
                     GTPHNTYFTFQGDEISTLFNFIRNIGLLPITNKSGEKFDDSFLNEIVLTKEQAVDLIL
                     KNPELVREIIKNNITTHEIQNLVYRKEQLEKFKNLLENEEFFNSTKKSFNKQIGDEYV
                     WQDFFENNTWIFGYGLNYIFNSPLQDKKLEQVVTGFDFNNSGKRIDALMKTRGLINSF
                     CFGEIKTHKKDLLKQVNDPYRGESWAISDELAGAIAQTQKSVQKSVNELSTKVEIKDK
                     IGNLTGEQIFIYQPKSFVVIGHLKEFITENGVNEDKFSSFELFRQNQTNPEILTFDEL
                     YERAKFIVKNGEEKTTNA"
     gene            complement(4103..4729)
                     /locus_tag="PLB46_09525"
     CDS             complement(4103..4729)
                     /locus_tag="PLB46_09525"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRF76799.1"
                     /translation="MTVKSSLDLRKKIKPCSNKICKACGLYLHQQPAYEKAETSHIFW
                     VGLSAVLFEEGIVPQPLSPLTPSGSLIDCIENPLKQEIQFYKTNIVKCVPLNTEKGKI
                     RYPFEHEMEKCFPNFEYEMEILTPTAVFLLGKQTASFVMKKFGVRNISFPDNFKYCSF
                     YINGIHFIPIHHPSYILVYKRKQMNSYVKNIQSLCKKLVEENRPAEAI"
     gene            complement(4677..>5300)
                     /locus_tag="PLB46_09530"
     CDS             complement(4677..>5300)
                     /locus_tag="PLB46_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF76800.1"
                     /translation="TVQKAIDVFSKFSLQWRSMAHVMTFNNVDANMMNDLKKSGCIEL
                     FIGIESGSPKILGEIKKTKNVDAILNNLTKVLQTGINMKGYFIYGFPDETKEDMEMTF
                     QLAVKLKQKSILNGVNFRTSVFQYRPYHGTQIYHDLELKGNNLLVQPVQPNQELSELV
                     GRLQFNFHSGNYSKVDDKVLHDYIYRTINLNDGKIFAGLEEKNQAML"
BASE COUNT         1542 a          893 c          824 g         2041 t
ORIGIN      
        1 atcagtggat cacattggtc cggaattggt ggtgcagtat gaccgaaata ttcaaaaaag
       61 cccgtatggt aaatattttt attattgatt gtgtcttgaa accgtatact gaaccattcc
      121 tgttgttcac ccctccagtc aaaccatttg atataaagtg tgtcgctgtg gcttgttgaa
      181 gcaattgtct cgatacggtt atagtaatta gttgtgtcaa aagtcaaaaa atatttttta
      241 tttgtttcgg taaatttccc ttctccatac caatattgca tatcatcagt tctactgcat
      301 tgtataaaag tcccaaattt atcagacttg ctttttttga tgaaagtgta attatttgaa
      361 aacaatgatg cgttcgcctg tcggtaaata gttgtgtctt gcgcgaataa ttgcccgaag
      421 gcagatatga ataatattgt gaatatgata tatattctgt tcatttatta atatctgcag
      481 ccggtttaac aatttgcatt ttagaaagca cttcgctttt ttcttaccaa cctctaacgg
      541 tttgcctagg tgctgttagc tgtagttttt tattccaatg aatgaaataa ttttgtcttt
      601 ggtgtcccat aagttacagt agttctttgt tcccctttgg cgaaattttc tctttgttct
      661 agctctgctt tgacttcagt ctccacgcct ttgagagcaa cctttatgag cttaattaat
      721 tctaatgtaa tgttattttt aaactgttca tcaaacccaa ctgtcccatt aaatgcttga
      781 tatgttttaa aattaaaaaa ggtttgctga tttctttggt ggtaaggggt tagctccttt
      841 cctatttcct taaatattag gggtggttcg tccatgttta ttttaacaaa tacacttaag
      901 aacatagcca atagtgagat ttcagcaaaa gagttttcat catttgaaaa cttaactagg
      961 ataaacattg gccttggaaa acctttggaa atatcgcttt ctagtttata aacataattc
     1021 ttgacagctt gtttttgtct catttccctg tcggtgtcaa aaaaaaataa tttagatttt
     1081 tcaattctat gtataagtcc gattgtttta attttttgaa agcactcatc tattatttta
     1141 tcgatacttt ctgaaatttg tttttttctt tcattctttt cttgattact gcgagttatt
     1201 ttttctattt cctcgaattt agttttatgg ctacttgtca ttgatagaat atcttcgaat
     1261 tctttgtcta tgcttgatat tccgtcaggg ttgaaggaac aaaagtctag tattcgtttg
     1321 ttggaattta ctgctaatct tttattccaa tctttaataa gtaagtctct tgataattgt
     1381 attgttgaaa atggcaaagt ttcatttgat attattggcg catcttcctt aattgcaaga
     1441 actaatttcg aatagggatt gtagtcatga aaaagctctt ttttggtgat taaatcatgc
     1501 ataaggccgc ctatttgata aagatttact gctttccatc cgtttaagga atcttcttct
     1561 gttcgaagta aaaattcagg tggagcatat cttaaagtgc caacaaattg cttttcatct
     1621 tggtcacttt gagaaggaac accaataagt tttaatacgc ccaaatccat cagaatgatt
     1681 tcattggctt tacttatcat aatattttca ggttttatgt ctctatgtac aattcccttc
     1741 agcaaaagtt cttcagttat agtgaatata gtctgaacga ctttttgaat aattgtatta
     1801 tcataatttt tttcgatgat aaattctttc aaattcaaac catcaatgta ttccatgatg
     1861 atataataat actcgtcagc gtcaatttga gatttacccc catcaatgat ttttatgagg
     1921 ttaagaattg tgtgtccttt aaggctaatt tctttttcta ttcttgattc ttgtatttcg
     1981 tgcccaaaac gttcgattaa ctcgttatca aaaattttta tagcaaattc attaccagaa
     2041 ttgtctttag ccttaaatac ggctgcggac ttaccattat taataaggtt aataatctca
     2101 tagcctaaaa agtctttgcc tttaagcgtt tcttctattt tttttgcttt gtatccgtcc
     2161 ataaaaatta cagctaacgt ttttgcgcca aacacatgtt aggcgtttgt ggttttttct
     2221 tcgccattct ttacaatgaa ttttgctcgt tcataaagtt cgtcaaaagt caatatttct
     2281 ggattagttt gattttgtct aaacaattca aaagagctga atttatcttc attcacaccg
     2341 ttttccgtta taaattcttt taagtgtcca attacaacaa aagatttggg ttgataaatg
     2401 aatatctgtt ctcctgttaa attaccaatc ttgtctttta tttcaacttt tgtacttagt
     2461 tcgttaactg atttttgtac agatttctgt gtttgagcta ttgctcctgc aagttcgtct
     2521 gaaatagccc atgattctcc acgatatggg tcattaactt gttttaataa gtcttttttg
     2581 tgtgttttta tttccccaaa acaaaatgaa tttattaatc ctcttgtctt cattaaagca
     2641 tcaattcttt ttcccgaatt attgaagtca aatcctgtca ctacttgttc caattttttg
     2701 tcttgaaggg gtgaattaaa aatgtaattt aaaccatacc caaaaatcca agtgttattt
     2761 tcaaaaaagt cttgccaaac atattcatca cctatttgct tgttaaagct ttttttagtt
     2821 gaattaaaaa actcttcatt ttctaacagg tttttaaact tttctaattg ttcttttctg
     2881 tataccaaat tttggatttc atgtgtcgta atgttgtttt taatgatttc tctaacaagc
     2941 tcaggattct ttaatattaa gtcaacagct tgttcctttg ttaatacaat ttcatttaag
     3001 aaactatcat caaatttttc tccagattta ttggtaattg gaagaagtcc aatatttcga
     3061 ataaaattga ataaggttga tatttcgtca ccttgaaatg tgaaataagt attgtgtggc
     3121 gttccggtgt ctgttgtaaa tttctgtatt gtaagagttg taattcctcg tgtgtcttcg
     3181 taaaatttag ctttaatttc ttgtctgcca ccaggggtta tgcgtaaggc aatttgtttt
     3241 ccgtctttta taaatttatg gtcttcgatg ttttcaatga ttttagaaac aatacgaaat
     3301 ggtctaattt tctcttcaag gtctcccttc tcgtttttgc ttaaaaattt ctcgtccaat
     3361 gcacgactca gatatactct atctgtgcgc ttattagtaa aataatcata ttctgttggt
     3421 tcttctttca tcggattgtt attttaacat aacgcctaac ttgattattt gagatgtgtt
     3481 acctaaatac cttctcaaat tctacttcag cggtgtgaat tgagtaggtt taccttttaa
     3541 aactacctca tatttttaaa attcatccat ttaatagtca aagaataatt tgaatttaat
     3601 cttgaatatt ttaccaccct caacacattc aacccacata tatcttgcaa gccacttatc
     3661 cactaaagct aaatgcttat tagtatttta ataaattact gcttcaaatt cacggaaaac
     3721 cgttaatttt agaaatgtca cattttaaaa ttatgagact aataaaatca ataccttcgg
     3781 gaaaaaggag ggcgttttta ggcattttac cttgtcacac tgaggtaaaa gttgtttttt
     3841 gtactgcaaa aagtcaactt cagttaaaaa taaaacattt ttccttctaa gcttagtatg
     3901 ttgcagcaaa aaaacacctt cacatcagtg aaagcaccaa accatttcat tccattatta
     3961 tttcaaaagt tgatctcgcc aggggacgcg ctgaagtaga atacaaagtc catccctgct
     4021 tttgcaaata cttttgttgc ttgcaggcgt attagagaaa ttcaaaataa caaatgagaa
     4081 aataaattta ccgtaaaagt tgtcaaattg cttctgccgg cctattttct tcaaccaatt
     4141 tcttgcaaag tgactgaatg ttttttacgt aagaattcat ttgctttcgt ttatacacta
     4201 aaatatatga aggatgatga atgggaataa agtgaatacc gttgatataa aaggagcagt
     4261 acttaaaatt atcagggaaa gaaatatttc ttacgccgaa ttttttcatt acaaaactgg
     4321 ctgtttgttt gcctagcaga aaaactgctg ttggagttaa tatctccatt tcatactcaa
     4381 aatttggaaa acacttttcc atttcatgtt caaatggata acggattttt cctttctctg
     4441 tgttgagggg aacacatttt acaatgtttg ttttgtaaaa ttgaatttcc tgctttaatg
     4501 gattttctat acagtctata agtgatcccg atggagttaa gggagataat ggctgaggta
     4561 cgattccttc ttcaaataga actgcggaaa ggccaaccca gaaaatatga gatgtttcag
     4621 ctttttcata cgcaggctgc tgatgtaaat acagcccaca tgccttacag attttattag
     4681 agcatggctt gatttttttc ctcaagtcca gcgaagattt taccgtcatt caagttaatt
     4741 gtgcggtaaa tataatcatg aagtacctta tcatcaactt ttgagtagtt gccgctatgg
     4801 aaattaaact gcaaacgccc tacaagctcc gatagttctt gatttggttg aaccggctga
     4861 actaaaaggt tattgccctt taattcaagg tcatggtata tttgagtgcc atgatacggc
     4921 ctgtattgga aaacactggt acggaaattt actccattta atattgattt ttgttttaat
     4981 ttaactgcta attgaaaggt catctccatg tcttctttag tttcatctgg aaagccataa
     5041 atgaaatacc ctttcatgtt aataccggtt tgaagaacct ttgttaaatt atttagaatt
     5101 gcatctacat tttttgtctt ttttatttcg cctagtattt ttggtgatcc ggactctata
     5161 cctatgaata attcaatgca tccgcttttc ttcaagtcat tcatcatatt ggcatcaacg
     5221 ttattgaatg tcataacatg tgccattgat ctccattgta aggaaaattt agaaaataca
     5281 tctattgctt tttgtacagt
//
LOCUS       DAORKW010000222         5295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_648_length_5295_cov_4.354389, whole genome shotgun sequence.
ACCESSION   DAORKW010000222 DAORKW010000000
VERSION     DAORKW010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_648_length_5295_cov_4.354389"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PLB46_09535"
     CDS             <1..1336
                     /locus_tag="PLB46_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRF76801.1"
                     /translation="YAEAAMQEHMAYLQTQFDKSWGKTEPWKNSKADVVAASRKRSSR
                     YTGLKAQGYSDKGITKMFDEPVETTIFTWDGPKDTIITPNDSLKHHLRFMHTGFMAME
                     PKTGYVLAWVGGIDHRFFKYDHVKSKRQVGSTFKPIVYATAIEQGISPCEYFPNQKIT
                     YDKYGWTPGNSSGEYGGYYSMMGGLTHSVNTVAAAIIMKTGVQPVIAEARKMGIKGDL
                     PQVPSIALGTASLSLQEMVTAYGCFANRGLKAEPQYLVRIETAEGEILYEHEDIKKTR
                     ALSQETADIMIQIMKSVVNNGTATRLRGTYGLGMDLAGKTGTTQNNTDGWFIGYNSRI
                     VAGAWVGAEDPAIRWRSTGLGQGAATALPIFGKFMYKSSRDSETRRYVTGGFTAPPDS
                     VMANLSCDMWIPDSISADSIGFIPSLINNIKEFLNKTGQDSLLPKYPGAEGK"
     gene            1338..1763
                     /locus_tag="PLB46_09540"
     CDS             1338..1763
                     /locus_tag="PLB46_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRF76802.1"
                     /translation="MAIWFNKNLSLSDFSHWNKNTLGEHIGIEFIELGENYLIAKMPV
                     DHRTHQPYGLLHGGASVALAETIGSVAAALVIDHDKFISLGIEINANHVKSVKSGFVY
                     GKTEPLHIGGSLHVWQITITDEKHQLICTSRLTVAIRKR"
     gene            1774..2661
                     /locus_tag="PLB46_09545"
     CDS             1774..2661
                     /locus_tag="PLB46_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76803.1"
                     /translation="MVAPSITPSTTAQLIAGLNQVESIRKKIRLNYTVIGTVLFIGLI
                     IEAITWMSIAVAIIILFVLVYTNWYGIPVSKFEKSFIETITKNTVGLIHPALQIDYQS
                     HLKLGEIASTGLINVKPDYFSGKNLIYGEIDQTAIRISEIYCHWKNTDHVRSDAKHIF
                     NGLVAKVENATNNLAPVEMSTNEQDLLLALQAQPNLQHGNWQNKVYYWSKSLTDKNIQ
                     FVNQHAQLLSNYTVQNHKQVKMGVDANTMMIAIINPGGFNYFNPSIFKTVFDTKSMET
                     YYSDLQFLFNCATTVNYSS"
     gene            complement(2649..2873)
                     /locus_tag="PLB46_09550"
     CDS             complement(2649..2873)
                     /locus_tag="PLB46_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76804.1"
                     /translation="MKRLFFLTIILTGISFMVSSCEKCTVCTASSLAGESVDEYCGNE
                     LDVRDFEDFFIDSVSDIEQAAYCERGPYDE"
     gene            2993..3307
                     /locus_tag="PLB46_09555"
     CDS             2993..3307
                     /locus_tag="PLB46_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007051681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HRF76805.1"
                     /translation="MIVTTTNNVEGKQITAYLGIITGETIIGANLFRDIFASVRDVVG
                     GRSGAYEDVLREAKDTALSEMTAKAVAMGADAIVGIDLDYETIRNGMLMVTASGTAVK
                     LS"
     gene            complement(3304..3693)
                     /locus_tag="PLB46_09560"
     CDS             complement(3304..3693)
                     /locus_tag="PLB46_09560"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRF76806.1"
                     /translation="MDSLQTRIDKSVTHTVKAIFPNTTNHYDTLFGGTALMWMDEVAF
                     ITATRFSRQKMVTVSSDRVDFKKPVPAGTIAELIARVSHVGTSSLLIKVEVYLEQMYS
                     DDREKAMEGTFTFVAIDENKQPVKISY"
     gene            3777..4538
                     /locus_tag="PLB46_09565"
     CDS             3777..4538
                     /locus_tag="PLB46_09565"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HRF76807.1"
                     /translation="MKKLFVITCSILGLTLPTQASNFPDTNENAIVEVVSTATLDDFF
                     TAANNFFIKYTTDGKVDYSGIKSEGTTIQKLYETIGAADLSNADKNTKTAFYLNAYNI
                     IVIHSVVKNMPVAKPTDVKGFFDATKHNVAGTYATLNDIENNKLRPDPRVHFSLVCAA
                     KGCPKIASEAFMPSTVETQLTALTKKAMNDASFIKVDNTNKVVKISQIFDWYGVDFVK
                     SNGSALSFINSYRDNKIPDTYKVEYYTYDWNLNGK"
     gene            complement(4563..5090)
                     /locus_tag="PLB46_09570"
     CDS             complement(4563..5090)
                     /locus_tag="PLB46_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76808.1"
                     /translation="MQILGVGSRIKHPDHGVGVVVAVHKRVYDICFIEKGIKAVLKDT
                     SLEIIEALESEYSVSFSEAEDALIKILREWSDVTEIIHLADKWDGGTMLLQPADKSLQ
                     AKEVPIDTFFHKIVMLRDRLRVLEQKINAQNKLSDSEKVEMQQYITRIYGSLTTFNVL
                     FKLKADYFVGDSSKE"
BASE COUNT         1740 a          944 c         1039 g         1572 t
ORIGIN      
        1 atatgccgaa gcagcaatgc aggaacatat ggcctatttg caaacccaat ttgataaaag
       61 ctggggtaaa acagagccct ggaaaaacag taaagccgat gttgttgccg cttctcgaaa
      121 acgcagcagc agatacaccg ggttaaaagc tcaggggtac agcgacaaag gcatcactaa
      181 aatgttcgat gaaccagttg aaacaaccat atttacctgg gacggcccaa aagataccat
      241 tatcacccca aacgattcat tgaaacatca tttacggttt atgcataccg gctttatggc
      301 aatggaacca aaaaccggtt atgtattggc atgggttggt ggtatcgacc atcgtttttt
      361 caaatacgac catgttaaaa gtaaacgtca ggttggcagt acttttaaac cgattgttta
      421 tgcaacagca atcgaacaag gtattagccc atgtgaatat tttcccaacc aaaaaattac
      481 gtacgataaa tatggctgga caccgggtaa ctctagcgga gaatatggtg gatattattc
      541 tatgatggga ggattaacac attcggtgaa tacagtagca gcagctatta taatgaaaac
      601 cggtgttcaa ccggttattg cagaagcccg taaaatgggt ataaaaggcg atttgccgca
      661 agttccttct attgcattag gaactgcgag tttgtcgttg caggaaatgg taaccgctta
      721 tggttgtttt gctaacagag gattaaaagc tgaaccacaa tatttggtgc gcattgaaac
      781 agcagaaggg gaaattttat atgaacatga agatattaaa aagacccgcg ctttaagtca
      841 ggaaacagct gatatcatga ttcaaattat gaaaagtgta gtcaataacg ggacagcaac
      901 cagattaaga ggaacatatg gtttgggtat ggatttagca ggaaaaacag gtaccacaca
      961 aaataataca gatggttggt ttataggata taattccaga attgttgcag gtgcatgggt
     1021 tggtgctgaa gatcctgcca tcagatggag gtcaacagga ttaggtcagg gagccgcaac
     1081 tgcactaccg atatttggca agtttatgta taaaagcagc agagatagtg aaacaaggcg
     1141 atatgttacc ggaggattta ctgctccacc tgattcagtg atggctaacc tgagttgtga
     1201 tatgtggatt ccggattcga taagtgccga ctcaataggt tttattccga gtcttattaa
     1261 taatatcaaa gaatttttaa acaaaacagg tcaggatagt ttattaccta aatatcccgg
     1321 tgctgaagga aaataatatg gctatttggt tcaataaaaa tctttcctta tccgatttca
     1381 gccattggaa taaaaatact cttggcgaac atattggtat cgagtttatt gaacttggtg
     1441 aaaattattt aatagcgaaa atgccggtgg atcatcgcac tcaccagcct tatggattat
     1501 tgcatggtgg agcatcagtg gcactggccg aaacaattgg cagtgttgca gcggcattag
     1561 ttattgacca cgataaattt atttctttag gtatagaaat aaatgctaat catgtaaaaa
     1621 gtgttaaatc tggatttgtg tacggtaaaa cggaaccatt acatattggt ggtagtttac
     1681 atgtatggca gattacaata acagatgaaa aacatcaatt aatttgtacc agcagactta
     1741 cagttgctat cagaaaaaga taatcaacaa catatggttg ctccatctat aactccttct
     1801 acaactgcac aattgattgc aggattaaat caggtagaat ccatcagaaa aaaaattcga
     1861 ctgaactaca ccgttattgg tactgtatta tttatcggat taattatcga agccattact
     1921 tggatgtcga ttgcggttgc gataataatt ttgtttgttt tggtgtatac caattggtat
     1981 gggattccgg tgagtaagtt tgaaaagagt tttattgaga ctattaccaa aaataccgtt
     2041 ggattaattc atcctgcatt acaaattgat taccagagtc acctcaaact aggtgaaatt
     2101 gcttctacag gtttgataaa tgtaaaaccg gattattttt ccgggaagaa tttaatttac
     2161 ggagaaattg atcaaaccgc tatccggata tccgaaattt attgtcattg gaaaaacacc
     2221 gaccatgtgc gaagtgatgc caaacatatt tttaatggtt tggtagcaaa agttgaaaat
     2281 gctacaaata atttagcacc agtggagatg agcaccaacg aacaagattt acttcttgct
     2341 ttacaagccc aaccgaattt acaacatggc aactggcaaa ataaggtgta ttattggtcg
     2401 aaatcattaa ccgacaaaaa cattcaattt gtcaatcaac atgctcaact attaagcaat
     2461 tatacagtac agaatcacaa gcaagtaaag atgggagttg atgcaaatac catgatgatt
     2521 gcgataatta atccgggagg atttaattat ttcaatccca gtatctttaa gactgtgttt
     2581 gatacaaagt cgatggaaac gtattacagt gatttgcaat ttttatttaa ttgcgcaaca
     2641 acggtaaatt attcatcata aggaccgcgc tcgcagtaag cagcctgttc gatatcagaa
     2701 acgctatcga taaaaaaatc ttcaaaatcg cgaacatcca gttcgttacc acaatattcg
     2761 tcaactgatt ctcctgctag tgaagaagct gtgcaaacag tacatttttc acatgacgac
     2821 accataaagc taatgcctgt aaggattata gtgaggaaga ataatctttt catgtttctc
     2881 gtttaggtta caaatataca aaaaccttta acgaaaatat atttccgctt attacgtcta
     2941 ttagtattat caacaagaat agtagattta tagtataaaa aagacaaaaa caatgatagt
     3001 tacaacaacc aataatgtag aaggcaaaca aattacagcc tatcttggta taataaccgg
     3061 tgagaccata attggtgcca acctgttcag ggacattttt gccagcgtgc gtgatgtagt
     3121 gggtggtcgt tcaggggctt atgaggatgt attacgcgaa gcaaaggata ctgcattatc
     3181 ggaaatgaca gccaaagcgg ttgccatggg tgcggatgct attgttggca ttgacctgga
     3241 ttacgaaacc atcagaaacg gtatgttaat ggttactgcc agcggaactg cagtaaaatt
     3301 gagttaatag ctgattttaa ccggttgttt attttcgtca attgccacga atgtaaaagt
     3361 gccttccata gccttttcgc ggtcgtcgga atacatttgt tcgaggtaaa cttctacttt
     3421 aattaataaa ctggaggtgc caacgtggct cacccgggca attaattcgg caatagtacc
     3481 tgcaggaacc ggctttttaa agtctaccct gtcgcttgat accgttacca ttttttgccg
     3541 actgaacctg gtggctgtga taaaagctac ttcatccatc cacatcaatg ctgtaccgcc
     3601 aaataaagta tcatagtgat tggtggtatt tgggaaaatg gctttcacgg tatgggtaac
     3661 cgatttatct attcttgttt gtaaactgtc catggcaaag tttttgatag gttatgcaaa
     3721 aataagccga actttatcaa aagggttcac gttatcttaa tatcttattg tatataatga
     3781 aaaaactatt cgtaattacc tgctctattt tgggtttaac attgcccact caggcaagca
     3841 actttccgga cactaatgaa aatgcaattg ttgaagttgt atcaactgcc actttagatg
     3901 atttttttac tgcggcaaat aatttcttta taaaatacac aaccgatggt aaggtagatt
     3961 attcaggaat aaaatcggaa ggaacaacta tccaaaaatt atatgaaact attggcgctg
     4021 cagatttaag taatgcagat aaaaacacta aaacagcatt ttacctgaac gcttacaata
     4081 ttattgtgat acattcggtg gtgaaaaaca tgccggttgc caaacctaca gatgtaaaag
     4141 gattttttga tgctaccaaa cacaatgttg ccggcaccta tgctacttta aatgatattg
     4201 aaaataacaa acttcgtccc gatccgcgcg ttcacttttc attagtatgt gctgctaaag
     4261 gttgtcctaa aattgcatct gaagcattta tgccatctac agttgaaaca cagttaacag
     4321 cgttgacaaa aaaagcaatg aatgatgctt cttttatcaa agtagataat accaataaag
     4381 tggttaaaat ttcgcagata ttcgattggt atggtgttga ctttgtaaaa tcaaatggca
     4441 gtgcattaag ttttatcaat agctatcgcg acaataaaat tccggatact tataaagttg
     4501 agtattatac ttatgattgg aatttgaacg gaaaataaat tggtaaggga ttattttagt
     4561 aattattcct tcgaagaatc accaacaaaa taatctgcct tcaatttaaa taacacatta
     4621 aaggttgtaa ggctgccata aatgcgggta atgtattgtt gcatttccac tttttctgaa
     4681 tcgctcaatt tattttgtgc atttattttt tgttctaata cccttaagcg gtcgcggagc
     4741 atcactattt tatgaaaaaa tgtatcgata ggcacttctt tggcttgcag gcttttatca
     4801 gcaggttgta gtaacattgt tcctccatcc catttatcgg caaggtgtat aatttcggta
     4861 acgtcgctcc attcgcgtaa tattttaatt aatgcatctt ctgcttcact aaagctgaca
     4921 ctatattcac tttctaatgc ttcaattatt tcgagtgaag tatcttttaa tactgcttta
     4981 attccttttt ctatgaaaca tatatcgtac acgcgtttat gtacggcaac tactactcct
     5041 accccatggt caggatgttt aatacgagaa cctactccta gaatttgcat agtcaatcat
     5101 gtttagggca aagataatca taacatgata accgaatacg cataaaaaaa cccgttcacc
     5161 aaagtgaacg ggtataggtg ctaaacacga cttcagcacc acacactaca atttttcaaa
     5221 acgacaaact ttcggcgcaa ggttgccaaa tctgacagcc aacagatagg aacctttacc
     5281 aagttcgcct atatc
//
LOCUS       DAORKW010000223         5272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_657_length_5272_cov_4.192064, whole genome shotgun sequence.
ACCESSION   DAORKW010000223 DAORKW010000000
VERSION     DAORKW010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_657_length_5272_cov_4.192064"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLB46_09575"
     CDS             <1..242
                     /locus_tag="PLB46_09575"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HRF76809.1"
                     /translation="GDAQKTTTENYIQQLEASGYYGDKIVTEVVPLMTFWPAEIYHQD
                     YFAKNPDQPYCVAVVGKKVAKFEKLFPELVKPEYK"
     gene            complement(243..896)
                     /locus_tag="PLB46_09580"
     CDS             complement(243..896)
                     /locus_tag="PLB46_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76810.1"
                     /translation="MKKTLLLIIICTIISQAFSQTGVYVGPRYSMIQSSWVTEYQPKL
                     GFGFGYATLTEFNDEFAFLYDVGVDRLGAEFTSFDYFFYPQRDEFNYAIHWWMAHIGI
                     SAAISINEDTRVFGGIDGGWIFSAAEDESINSDDYMYYSLDGTPEGLDVFKIESGLDQ
                     AYTGDLHLGGSMTFGILTVRAKYSYQLINNTLTSHFLEPDDFRNNVISLDLLLGWRD"
     gene            complement(924..1310)
                     /locus_tag="PLB46_09585"
     CDS             complement(924..1310)
                     /locus_tag="PLB46_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRF76811.1"
                     /translation="MPELTQKCRIDKWLWAVRLYKTRNLAAEAVKTGKVKVKGDNIKP
                     SFEVYEGLVITIPKGVVRYQFKVVGVIEKRVSAVLAKNYCEDVTPEEEKMKLTHVEFM
                     PTAFRDKGTGRPTKKERRDLDEWMND"
     gene            1379..1726
                     /locus_tag="PLB46_09590"
     CDS             1379..1726
                     /locus_tag="PLB46_09590"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HRF76812.1"
                     /translation="MQKPISAREIVLSLKERFRPEKADPGYETIFHLDISGERGGQFT
                     VRISDGNLNIEEGLHDSPKCIVTTKDETYEDMEWGRTNAQMAVMFGKVKVSDLGEILD
                     FIPLFHQCADFYK"
     gene            1747..3426
                     /locus_tag="PLB46_09595"
     CDS             1747..3426
                     /locus_tag="PLB46_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76813.1"
                     /translation="MNKKITAFILSLCTLSVFAQNDGAFNGMSVSTGNLHPESSNYGV
                     VLQNELIQVTEFDDEVKYDVVYEFKNATTNFATVSAVMPLKIYFNEFGYGKRAKLLDD
                     LATVPTFSDIFAVQDRSVDTREQIRKNFQQRLFVRKYISIDNLKQLGIHVDMFRNNTR
                     INIKKVMCELKFTDATPLYLPKNTEVLSMEISFIIEMNFTPDEETNVLTFITIPSVNA
                     GIDRTESYSMYELGYEKNWSGRLNGLYLQHDIFKTTPILPSKMSEFKTHVSGERDQVV
                     AFEDIMLVDKDKIAFFHLSNNKACNSGNLFNEQSIIPAAIKNITASSWVKTDAEMPKR
                     FFYNTPMIAFSDSLHPYQTGTLTGIDALNNATKITNYNNLSYYDYIKSNCKSNVPGIS
                     LKESGNPVYAFDISDHIYEDSTYEGRENLARQTCWCEAAAGPGTGEYIEFEITQPARA
                     MKIYNGNQSSKKVFDESSKADIIVITSLDGHKINPNADNPSIMRSSIIDLTILNVYEL
                     NLLPGKYRVAIDAVDKGITPVTCFSTIAFDFEVRDEWYLKSQGMLRAFFKK"
     gene            complement(3503..4621)
                     /locus_tag="PLB46_09600"
     CDS             complement(3503..4621)
                     /locus_tag="PLB46_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HRF76814.1"
                     /translation="MSKDLKITPISEWGWNFQTPLIMTGPCSVESEEQVRETAKQLSS
                     HRFDILRGGIWKPRTRPNSFEGIGSIGLKWLKDAGNEIGKPVATEVANVKHVYEALRM
                     GIDVLWIGARTTVNPFAVQEICDALQGVDIPVLVKNPINPDLELWIGAFERLNRSGIT
                     KLAAIHRGFSTHEKSKYRNIPRWNIPIELKRRYPQLPIVCDPSHICGTREYLREVAQT
                     ALDIGFEGLHLESHINPDKALSDAKQQITPDAFGEMMLQLHTRESIEHNPIAENQLEI
                     LRGKIDNLDNYLLEMMSERMDIVREIAEYKRENNLAIVQPSRWTEILENRLETGKRKQ
                     LTEKFIKELFHAMHKESIHHQTEVMLLNGEKSEVEKKA"
     gene            complement(4692..>5272)
                     /locus_tag="PLB46_09605"
     CDS             complement(4692..>5272)
                     /locus_tag="PLB46_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HRF76815.1"
                     /translation="VIGEIIAPIELHLAAKSPISLSDLKTVYSHQMAIKESKHFFEQY
                     PNIEFVNTPSTASAVKLVATSVQQNIAAIGNKSAIAYYDLQVIAEKIDNHAANFTRFL
                     ILSNNKATGNTTFSDGKASLLLQPGKTGLPEALIVHPNISLLRHLENGNVYIETVFLT
                     SQQIINMLSNFEQNGLSVSILGVFPIGNTMVI"
BASE COUNT         1630 a          982 c          984 g         1676 t
ORIGIN      
        1 atggtgatgc acaaaaaact accaccgaaa attatataca acagctcgaa gcaagtggtt
       61 attatgggga taaaattgtt actgaggtag tgccattaat gactttttgg ccggcagaaa
      121 tttatcatca ggattatttt gcaaaaaatc cggaccagcc atattgtgtt gctgtagttg
      181 gaaaaaaagt ggcgaaattt gaaaaattat ttcccgagtt ggttaaaccg gaatacaaat
      241 aattaatctc gccaacctaa aagtaaatcc aggcttatta cattatttct gaagtcatca
      301 ggttccagaa aatgtgatgt caatgtattg ttgatgagtt ggtagctgta ttttgctctg
      361 actgtcaata taccgaacgt catgcttcca ccaaggtgta aatcgcctgt gtaggcctgg
      421 tccaatcctg actcaatttt aaatacatct aaaccttcag gggtgccatc gagtgaataa
      481 tacatgtagt catctgaatt aatgctttcg tcttctgcag cagaaaatat ccagccgcca
      541 tcaattccac caaacacgcg tgtatcctca ttaatactga ttgctgcgct gatgccaata
      601 tgtgccatcc accaatgaat agcataatta aattcgtccc tttgaggata aaaaaagtaa
      661 tcgaaactgg taaattctgc gccgagccta tcaacaccaa catcatataa aaatgcaaat
      721 tcatcattga attcggttaa agtagcataa ccaaaaccaa atcctagttt aggctggtat
      781 tcagttaccc aggaactttg aatcattgaa tatctcggac cgacataaac gccggtttgt
      841 gaaaatgctt gtgaaattat cgtgcaaatt ataatcagta atagtgtttt tttcatatag
      901 ttctttacta tgaaattttc tttttaatca ttcatccact catctaaatc tctgcgttct
      961 ttttttgttg gtcttcccgt gcctttgtcc ctgaacgctg ttggcataaa ttccacatgt
     1021 gtgagtttca ttttttcctc ttcaggagtt acatcctcac aataattttt cgctaaaacg
     1081 gcacttaccc gcttttcaat aacaccaact actttaaact ggtagcgtac aacacctttc
     1141 gggatggtaa ttaccagccc ttcatacacc tcaaaagaag gttttatatt gtcccctttc
     1201 acttttacct tgcctgtttt aaccgcttca gcagccaaat tgcgggtttt atacaggcga
     1261 acggcccata accatttatc gattctacac ttttgagtaa gttcaggcat gatgattgcg
     1321 taaagtcacc gtaaaaataa actacattat tttatcttag cggcaatatt gaccaaacat
     1381 gcaaaaacca atctcagcgc gcgaaattgt gctgtctcta aaagagcgat tcaggcccga
     1441 aaaggctgac ccaggttatg aaactatttt ccatttagac atctcaggcg agcgtggtgg
     1501 acaattcacc gtgcggatta gcgatggaaa ccttaacatt gaggaaggat tacatgacag
     1561 tccgaagtgc attgtgacaa ccaaagatga gacttatgag gacatggaat gggggagaac
     1621 caatgcccaa atggcagtga tgtttggaaa agtgaaagtt agtgacttag gagaaatcct
     1681 ggactttatt cctttattcc atcaatgtgc cgatttttac aaatgagatt ttaaacatca
     1741 atagctatga ataaaaagat tacagccttt attttgagtt tgtgtacact ttctgttttt
     1801 gcgcagaacg acggcgcctt caacggaatg tcagtttcta cgggtaacct gcatcctgaa
     1861 tcttccaatt acggcgtagt gctgcaaaac gaattgatcc aggttacaga attcgacgac
     1921 gaagtgaaat atgacgtagt gtacgaattt aaaaatgcta cgaccaactt tgctaccgtg
     1981 agtgccgtta tgcctttaaa aatttacttt aatgagtttg gctacgggaa acgagcaaaa
     2041 ttattggacg atttagcaac agtgcctacg ttcagcgaca tttttgctgt gcaggatcgt
     2101 tcagtggata cgcgtgaaca aattcgtaaa aatttccaac aaagattatt cgtaagaaaa
     2161 tatatcagca ttgataacct aaagcaattg ggtattcatg tagatatgtt tagaaataat
     2221 acacgcatca atattaaaaa agtaatgtgt gaattaaaat ttacggatgc tacaccattg
     2281 tatttgccga aaaatacaga agttttatcc atggaaataa gttttattat tgaaatgaat
     2341 tttacacctg atgaagaaac aaatgtgtta acatttataa caattccaag tgtaaatgcc
     2401 ggtatcgata gaacagagtc gtattccatg tatgaattag gttatgaaaa aaactggtca
     2461 ggtcgtttaa atggattata cttacaacat gatattttta aaactacgcc aatcctgcca
     2521 tctaaaatga gcgaattcaa aacgcatgtt agtggcgaac gtgatcaggt tgttgctttt
     2581 gaggacataa tgttagtgga taaagataaa attgcttttt tccatctcag caataataaa
     2641 gcatgtaatt ctggcaattt gtttaacgaa caatcaatta taccagcggc aattaaaaat
     2701 attactgctt caagttgggt gaaaactgat gcggaaatgc ctaaacggtt tttttataat
     2761 acgccaatga tagcgttttc agactcgctc catccatatc aaacaggtac gttgaccgga
     2821 attgatgcat taaataatgc aactaagatt actaactaca ataacctgtc gtattatgat
     2881 tatattaaat caaattgtaa aagcaatgta cccggaattt cattaaaaga atcgggtaac
     2941 cctgtctatg catttgacat ttcagatcat atttatgaag acagcacata tgaaggacgt
     3001 gaaaacctcg ctcgtcaaac gtgttggtgt gaagctgctg caggtcccgg aaccggtgag
     3061 tatattgaat ttgaaattac tcaacctgct cgtgcaatga aaatttataa tggtaatcaa
     3121 tccagtaaaa aagtatttga tgaatcttca aaagcagata ttattgttat taccagttta
     3181 gacggacata aaattaatcc aaatgccgac aatccttcaa ttatgagaag ttcgattatt
     3241 gatttaacca ttttaaatgt ttatgagtta aatctattac ctggtaaata ccgtgtggca
     3301 attgatgcag ttgataaagg cattacacct gtaacctgtt tttctacaat tgcgtttgat
     3361 tttgaagtaa gggatgaatg gtatttgaaa tcacaaggta tgctgagagc attctttaaa
     3421 aaataatatt attctaaaat attaccaata aaaaatccgg aacaattgct ccggattttt
     3481 tttatgttgt ggtttgttct aatcatgctt ttttctccac ctctgacttc tcaccattaa
     3541 gcaacatcac ttcagtttga tgatgaatag attccttatg catagcatgg aataattctt
     3601 taatgaattt ttcggttaat tgtttccgtt taccggtctc cagtctgttt tccaaaattt
     3661 cggtccatct acttggctga acgatagcta aattgttttc gcgtttatat tctgcgattt
     3721 cacgcacaat atccatacgt tcgctcatca tttctaacaa atagttatca aggttgtcga
     3781 ttttaccacg taaaatttcc aattgatttt cagcaatcgg attatgttcg attgattccc
     3841 tggtatgcaa ttgcaacatc atttcaccaa aagcatccgg tgtaatttgt tgtttggcat
     3901 cgcttaatgc tttatccggg ttgatatgcg attcgagatg taatccttca aaaccgatgt
     3961 caagtgcagt ttgagcaacc tcgcgcaaat actcacgtgt tccacaaata tggcttgggt
     4021 cacaaactat tggcagttgt gggtaacgac gttttaattc aattggtatg ttccaacgtg
     4081 gaatattgcg gtatttcgat ttctcgtgag tgctaaagcc gcgatgaata gcagcaagtt
     4141 tggttatgcc actgcggttt aaacgctcaa aagcgcctat ccaaagctct aagtccgggt
     4201 tgatagggtt ttttactaat actggtatat caacaccttg taaagcatca caaatctcct
     4261 gaacggcaaa tgggtttacg gttgttcttg cgccaatcca taaaacatca attcccatgc
     4321 gaagtgcttc gtaaacgtgt tttacgttag ccacttcagt agcaacaggt tttccaattt
     4381 cattaccggc atctttaagc cattttaaac caatgctgcc aataccttca aaagaattag
     4441 gtcttgttct tggtttccaa ataccaccac gcaaaatgtc gaagcggtga ctacttaatt
     4501 gtttggcggt ttcacgcact tgttcctcac tttcaacact gcaaggacct gtcataatca
     4561 gcggagtctg gaagttccat ccccattctg atataggtgt aatttttaaa tctttactca
     4621 tcttagttcg tattttgtgt ttaatgttga tagtatgtgc acaaagtaag taatttttta
     4681 acaaacatca atcatataac catggtgttg ccaataggaa aaacgcctaa aatgcttact
     4741 gataggccat tttgctcgaa attggacagc atattaatta tttgttgcga tgtcaaaaaa
     4801 acagtctcga tatacacatt cccgttttcc aaatgcctga gaagggagat atttggatga
     4861 acaatcaacg cttcaggtaa acctgtttta cccggttgca ggagtaaaga cgcttttccg
     4921 tccgaaaagg ttgtattgcc ggttgcttta ttattcgata aaattaaaaa acgagtgaaa
     4981 tttgctgcat gattgtcaat tttttcggca attacttgca aatcataata ggcaatggcc
     5041 gatttgttac caattgctgc gatattttgt tgaacagaag ttgcaacaag tttaacagca
     5101 cttgccgttg aaggggtatt tacaaactcg atattagggt attgttcaaa aaagtgtttg
     5161 gactctttta ttgccatttg gtggctgtat acggttttga ggtcactaag tgaaataggt
     5221 gatttggctg caaggtgcag ttctattgga gctataattt ctccaattac ag
//
LOCUS       DAORKW010000224         5269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_659_length_5269_cov_5.078251, whole genome shotgun sequence.
ACCESSION   DAORKW010000224 DAORKW010000000
VERSION     DAORKW010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_659_length_5269_cov_5.078251"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PLB46_09610"
     CDS             complement(<1..506)
                     /locus_tag="PLB46_09610"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRF76816.1"
                     /translation="MLKSLLARTKAVFLITGFLMLGNAAFADYCIPDYVTGTGDDDYI
                     DGVELGDISNFSGPGDDWNDFTDFSTTLMPGMTYDLTVYNTPSWQEEYMAWIDYNQDE
                     IFSDDERLTPDNVVLTEGASAVISFTVPFGVLPGATRMRVMCVYFPGALIEPCPEGTY
                     NYGEAEDYT"
     gene            635..2110
                     /locus_tag="PLB46_09615"
     CDS             635..2110
                     /locus_tag="PLB46_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRF76817.1"
                     /translation="MSRPLNDITKMRPSVLIIDDDPTFCLMLKSFLSKKDFATETAFS
                     GAEAINQVKNNNFDIVLSDFRLPDTDGLELLENIREIKPGTPVIIMTSYADIRIAVKA
                     MKIGAYEYVTKPVNPDEILLTIKNALNRDKDNLVVPLPPTQQTGKIEQPTGKKSTLKN
                     GFEYVEGNSDTANRIMQYIDVVAPTNLSVIINGESGTGKEYVAKMIHQKSNRSHKPFV
                     SIDCGALSKELAASELFGHIKGSFTGAISDKTGQFVFADGGTIFLDEIGNLTYEIQVQ
                     LLRAIQERRIRKIGSNQDVEIDVRIIVATNEDLMEAVKKGNFREDLYHRLNEFTILVP
                     SLRDRGTDIDIFAGHFLNLANTELSKSVDDFDEEVKTIFRKYSWPGNLREMRNVVRRA
                     VLLTSGNLVKKETLPHEIVFEKREEANVNNNIHSTPQNNVEQPSDLKSLAEKTERELI
                     INTLVKVNYNKSKAATLLKIDRKTLYNKMKLYKILTGNEDE"
     gene            complement(2112..4742)
                     /locus_tag="PLB46_09620"
     CDS             complement(2112..4742)
                     /locus_tag="PLB46_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF76818.1"
                     /translation="MSEKAPSTGLTWKLIAGFILSTALIGFAAWFAYQNFNKLLGSVE
                     TLSNPNNSAEELQNLIYSMHEADANMRNYIMTDDPGAFQSYMTMMETMSVELDDLKQD
                     RNFASNELSLDSLEDYLNDKADLMYSLVIFKKSSEYERLNSKALAKISSNLEDEGGFT
                     SSKSDNVATKTPTIKQPSIKQAESTTDKSKKTKKEKDKDEGFLKNLFSGKNKNNKTEA
                     SDPTIVALKDTTNNSRSTAELQFDIANSPSVDVEDVRQVLKEIENEQRQYNTTLTIKE
                     LEILASDKIIIKKINELIDRIKAEQITLQQAQLIAANNNAKRSSETMLLIASIALLTG
                     LVFMGIILVDINRSNRYKKELIIARNRAEYLAHAKEEFLANMSHEIRTPLNAIIGFSE
                     QMESTPLQPSQKKYIKAVSNAGAHLLNTVNDILDLSKIEAGKLAIDDQPFKLKDAIDE
                     VVSIMEIKAKEKQLALSVLPIDLANEYVIGDAFRIKQILYNIAGNAIKFTEQGKIEIS
                     GSAKKHKTLIEYHIHITDTGVGIAHDKIDKIFESFEQAENSITRKFGGTGLGLSISKK
                     LAALIGGDINVKSEINKGSTFTITLPLPLADEKTISTIIQEPTSHIDLHGMEILVVDD
                     EPFNLMLAEVILKKYGAIITVAVNGKEAFDKIELQNFDIVLADLHMPEVDGYMLANKI
                     REKYLQVPLIALTANVMQNDMVKIKKSGFNDILLKPYKEKALLDIIAKYAPVVIGENE
                     LDESTNKLTTDTEHQHTMESHTLYSLDEIRKFADNDNSILVAIVESFIQNNSLNLFTL
                     KDATEKNDLHVVRNTAHKMLPSYNHFHVHALISDLKKLEIVEEISPVDLINCYYHIEK
                     VSKEVFTLLEQDCRTLTFAK"
     gene            4888..>5269
                     /locus_tag="PLB46_09625"
     CDS             4888..>5269
                     /locus_tag="PLB46_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF76819.1"
                     /translation="MQNEKIKILIVDDEPDILEVIEYNLLKDDYQVVRASNGKIALEK
                     AIAEKPNLILLDIMMPEMDGIEVCRELRSLREFDNTIIAFLTARNEDFTQVQGFDVGA
                     DDYITKPIKPRILLSRIKELLRRTA"
BASE COUNT         1679 a          902 c          968 g         1720 t
ORIGIN      
        1 gtgtaatctt ctgcttcacc gtagttgtag gtaccttcag ggcaaggttc tattagtgca
       61 cctggaaaat aaacacacat tacgcgcatt ctggttgctc ccggtaaaac gccgaaagga
      121 accgtaaatg aaattacagc tgatgcacct tctgtcagca ccacattgtc aggtgtcaat
      181 cgctcatcgt cgctgaaaat ctcatcctga ttataatcaa tccacgccat gtattcttct
      241 tgccatgacg gagtattata cacagtaagg tcgtaagtca tacccggcat gagggtggta
      301 gaaaaatcag taaagtcatt ccagtcgtcg cccggacctg agaagttaga aatatcgcca
      361 agttcaacac cgtcgatata atcatcatca ccggttcctg taacataatc tggaatacaa
      421 taatcagcaa atgctgcgtt tcctaacata aggaatcctg taatcaggaa aacagcctta
      481 gttcgggcaa gtaaagattt taacataagt cgcaaattag tatgaaaaga aagaggctgt
      541 aaaagtatat aaggatgttt agaaatggaa aaaaatttta ttcaccaata atccccacat
      601 tttgtcgtgc ctgtttgact aagttggact atatttgagc cgtcctttaa atgacattac
      661 caaaatgcgc ccatctgtac tcataattga tgacgaccca actttttgcc tcatgctgaa
      721 aagctttttg agtaagaagg attttgctac agaaacagcc ttttccggtg cagaagcaat
      781 taatcaggtt aaaaacaaca atttcgatat tgttttaagc gattttagac taccggatac
      841 tgatggattg gaattattgg agaatattcg ggaaataaaa cccggtactc ctgtaatcat
      901 catgacttcc tatgcagata ttcgaatagc tgtaaaagcg atgaaaattg gtgcatatga
      961 atatgtaact aaacctgtga atccggatga gattttatta acaattaaaa atgctttaaa
     1021 ccgagataaa gacaatttgg ttgtgccttt gccgccaact cagcaaacag gcaaaattga
     1081 acaaccaacg ggtaaaaaaa gtactttaaa aaatggattt gagtacgtag agggcaatag
     1141 cgatacggca aatagaatca tgcaatatat cgatgttgtt gcgccaacca atttatctgt
     1201 aatcattaat ggagaaagcg gaactggtaa ggaatatgtg gcgaaaatga ttcaccaaaa
     1261 gagcaatcgt tctcataaac cttttgtatc aattgattgt ggagcactaa gtaaagaact
     1321 cgcagcgagt gaattgttcg gacatataaa aggatcattc acaggcgcta tttctgacaa
     1381 aacagggcaa tttgtttttg ctgatggcgg taccatattt ttggatgaaa ttggtaattt
     1441 aacttacgaa attcaggtgc aattgttacg cgcaattcaa gaacgaagaa ttagaaaaat
     1501 tggtagtaat caggatgtag aaattgatgt tagaataatt gtagccacga atgaagattt
     1561 aatggaagcg gttaaaaaag gcaatttcag agaagattta tatcaccggt taaatgagtt
     1621 taccattcta gttccatcgt taagggacag aggtaccgat attgatattt ttgccggtca
     1681 ttttttaaat ttggcgaata cagaattaag taagtcggtg gatgattttg atgaagaagt
     1741 taaaacgatt ttcagaaaat attcatggcc aggcaattta cgtgaaatgc gcaatgtggt
     1801 gcgcagagca gtattactca ctagcggaaa tttggttaaa aaggaaacgt tgccacacga
     1861 aattgttttt gaaaaacgcg aagaagcaaa tgtaaataac aacattcatt ctacaccaca
     1921 aaataatgtt gagcaaccta gtgatttaaa atcgttggcc gaaaaaacgg aacgcgaatt
     1981 aatcataaat acactggtaa aagttaatta caataaaagt aaagccgcta ccttattaaa
     2041 aatagaccgt aagacattgt ataataaaat gaaattatac aaaatattaa ccggtaatga
     2101 ggatgaataa attatttagc gaatgttaaa gtgcggcaat cctgttccaa caaggtaaat
     2161 acttccttgc tcactttttc aatatgataa taacaattaa ttaaatcgac tggagatatt
     2221 tcttccacga tttccaattt ttttaaatca cttattagcg cgtgaacatg aaagtgatta
     2281 taagatggta acattttatg tgcggtgttt cttactacat gtaaatcatt tttttcagta
     2341 gcatctttta atgtaaataa atttaacgaa ttgttttgaa taaacgattc cactatagct
     2401 accagaatac tattatcatt atccgcaaat tttctaattt catcaaggct gtataaagta
     2461 tgagattcca tagtatgttg atgctcggta tcagttgtca atttatttgt tgactcatct
     2521 agttcgtttt cacctattac aaccggtgca tattttgcaa ttatatccaa taaagccttt
     2581 tctttataag gcttcaaaag gatgtcatta aagccacttt ttttaatttt caccatgtcg
     2641 ttttgcatca cattggcagt taatgcaata agtggaactt gtaaatattt ttcacgaatt
     2701 ttattagcca gcatatatcc atcaacttca ggcatatgca aatcggctaa aactatatcg
     2761 aaattttgta actcaatttt atcgaatgct tctttgccat ttacagcaac agtgataatg
     2821 gcgccgtatt tttttaaaat tacttcagcc agcattaaat taaatggctc atcgtcaacc
     2881 actaatattt ccatgccatg taaatcgata tgagaggtag gttcctgaat aatggtgcta
     2941 atagtttttt catcagccag tggcagcggg agcgtaattg tgaatgtact tcccttatta
     3001 atttcgcttt ttacattgat gtcgccacca atcaatgcag ccaatttttt tgaaatactt
     3061 agtcctaaac ctgtaccacc aaatttccgg gttattgaat tttcagcttg ttcgaagctt
     3121 tcaaatattt tatcaatttt gtcatgggca attccaacac cggtatcggt aatatgtata
     3181 tgatattcaa ttagggtttt atgttttttt gcagaaccag aaatttcaat cttaccttgt
     3241 tcagtaaatt ttatagcatt acctgctata ttgtaaagta tttgtttaat acgaaatgca
     3301 tcaccaatta catattcatt tgctaaatca atcggtaaaa cggataacgc tagttgtttt
     3361 tcttttgctt tgatttccat aatggaaact acttcatcaa tagcatcttt tagtttgaat
     3421 ggttggtcat caatagctaa tttaccagcc tcaattttag ataagtcgag gatgtcgtta
     3481 acggtattta ataaatgcgc acctgcgtta ctaacggctt taatatattt tttttgtgat
     3541 ggttgcaatg gcgtgctctc catttgttcg ctaaaaccta taattgcatt aagcggagtg
     3601 cgaatttcgt gactcatatt tgctaaaaat tcttccttgg catgtgccag atattcagcg
     3661 cgattgcggg caattataag ttctttttta taacggttac tccgattaat atctaccaaa
     3721 attataccca taaaaaccaa tccggttaat aatgcaatag aggcaattaa caacatggtt
     3781 tcagatgaac gtttagcatt gttgttagcc gcaattaatt gagcttgttg tagcgtaatt
     3841 tgttctgcct tgatacgatc aattagttca tttatttttt taattatgat tttatcgctg
     3901 gctaatattt caagttcttt aatagttaat gtagtattat attgtcgttg ttcattttca
     3961 atttctttaa gcacttggcg aacgtcctct acatccacag aagggctatt ggcaatatca
     4021 aactgtaatt ctgccgttga acgtgaatta ttagttgtat cctttaaggc aacaatagtt
     4081 gggtcggagg cttctgtttt attattttta ttttttcctg aaaataaatt cttcaaaaaa
     4141 ccttcgtcct tatccttctc tttttttgtt tttttggatt tatctgtagt actttcggct
     4201 tgtttgatgg agggttgttt aattgttggt gttttggtgg caacattgtc ggatttcgaa
     4261 ctggtaaaac ctccttcatc ttcaagatta gatgaaattt tggcaagcgc cttactgttt
     4321 aaacgttcat attcacttga tttcttaaaa atcaccaaag aatacatcaa atcggcctta
     4381 tcattaagat aatcttccag tgaatcaagg ctcaattcat ttgaagcaaa attcctatcc
     4441 tgttttaggt catcaagttc aacgctcatg gtttccatca tggtcatata actctggaaa
     4501 gcccccgggt catctgtcat gatataattg cgcatgttgg catccgcttc atgcatactg
     4561 taaatcaagt tttgcagctc ttctgccgaa ttgttaggat tagaaagtgt ttctaccgaa
     4621 ccaagcagtt tgttgaaatt ttgataggca aaccaggctg caaatccaat aagtgcagta
     4681 gacaagatga accccgctat caatttccac gttaaacccg ttgaaggtgc cttttcactc
     4741 atagttctgc gtttaaagtt acatgattta gctcaaatgt gtgaaatccg gaacttatac
     4801 gtaaattaaa tgtttcttta acattaactt aacacgattt tgagtttgag ttaatactcg
     4861 ctatttagct ttacatcaaa attgcatatg caaaacgaaa agataaagat tttgattgtg
     4921 gatgatgagc cggatattct ggaggtaata gaatacaacc tgttaaagga tgattatcag
     4981 gtagttagag cttcaaacgg caagatagct ttggaaaagg ccatagcaga aaagcccaat
     5041 ttaatattgt tagacattat gatgcccgaa atggatggta tagaagtgtg ccgtgagttg
     5101 cgcagtttaa gggagtttga caataccatt atcgcatttt taacagccag gaacgaagat
     5161 tttacccaag tgcagggttt tgatgttggt gcagatgatt acatcactaa gcctataaaa
     5221 ccgagaatac tgttgtcaag aataaaggaa ttgttgcgga gaacagctg
//
LOCUS       DAORKW010000225         5250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_663_length_5250_cov_4.760154, whole genome shotgun sequence.
ACCESSION   DAORKW010000225 DAORKW010000000
VERSION     DAORKW010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_663_length_5250_cov_4.760154"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="PLB46_09630"
     CDS             <1..1424
                     /locus_tag="PLB46_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF76820.1"
                     /translation="NDAPVQDARLLPQANYYGGDLAGIQQKIDDGYFSNLGINSLWIS
                     PVFQNPYSAYQEFPEPHRWFSGYHGYWPISLSKVDTRLGTNTEFKQLVSGAHNSNMSV
                     YLDYVANHIHAEHPLWKSNPEWFSQLDLPDGRKNLRLWDEYRLTTWFEPYMPSFDHSK
                     PIVAEACADSAMYWLKEFNLDGFRHDATKHIETEFWRLLTYKIKTEIEIPQNRNIYQI
                     GETFGSRELISSYLGSGLLNAQFDFPLYFDARSVFANNEVGFDRLNTSLHETFDAYGY
                     HHVMGNITGNHDMARFISYASGDLKWDENDKEAGWNRNITVTDTIGYAKLKMLHAFNM
                     TIPGIPIIYYGDEIGMPGANDPDNRRMMRFDNLNPFETDVKNTVTELTKLRISHMALL
                     YGDFEVLMVNATQYVYKRTYLNDEVIVVFNKSPQKTTIIVNDTISKNKYQPNFGNIIV
                     VSDNSIEVTLAPYSFEIITLKNN"
     gene            1435..2751
                     /locus_tag="PLB46_09635"
     CDS             1435..2751
                     /locus_tag="PLB46_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF76821.1"
                     /translation="MKKIAFVLFALVLTQLTFAQAPEWTKDKTIYEVNIRQYTQEGTF
                     DAFATHLPRLEKMGVGILWLMPVQPIGELNRKGSLGSYYSIANYTATNPEFGSMKDFK
                     KLVDEAHKRDMTIILDWVANHTSFDHVWMKSHPDFYTTDKSGNVIPPVADWSDVADLN
                     YDNKAMRAAMIADMKFWVEEADIDGFRCDVAGMVPDDFWMEALTELKKIKPDIFLLAE
                     AEGPQFHTDGFHMTYGWSKHHIMNEIAKGKALADRMDSIIVNHNSVYPTGSYYMNFTS
                     NHDENSWNGTEFERMGEGAKTFGVMAATIPGMFLLYTGQEVALDRRLKFFDKDSVVWL
                     DNRNFTQFYTTLIQLKADNPALWNGAYGGDYKRIDTQHEKIYAYSRTLGDNKVVVILN
                     LSSDAHDAVLNTDSGKYKNVFTGKKQKLKAGKKITVPAWGYVVLKQ"
     gene            2823..4328
                     /locus_tag="PLB46_09640"
     CDS             2823..4328
                     /locus_tag="PLB46_09640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF76822.1"
                     /translation="MFDNTIGILILVLATILYLIAFYYSRQDKIKLAVLLVVLAGFII
                     RCFVASDPYLHPWDERYHALVAKNIISEPLKPTLYEDPVLPYNYKQWHKNHIWVHKQP
                     MPLWMMAASMRVFGVNEFALRMPSVLLSTLGIWLIFLVGIYFYSHKVAFISAFLFSLN
                     GLIIELTGGRVATDHYDLLFLIFILSAIYFTTKFVTTKSVIFNILAGLMIAFAILTKW
                     LPALIVLPIWLILSYSSKTFSIAEMAKHFALLVGVIIIVSSPWQIYIANVFPAETAWE
                     QHYNRLHIFEILGGRDEPWWFFLERLRINYGELVYIPLVYFLYKLFNKTSSIKYYSLA
                     IWIIIPLVFFTLVKTKMQGYILFTSPAIFMITGWFIFYMQDQNLQGWKKYLSRFVIAL
                     LLLLPVRYGIERSKLLDPPSKDYNWVAELRALDKDIQQNSVLFNYNMPIDAMFYTEFM
                     VYQQLPDDKTIFDLQARGYNVYVAYEGEIPSGFENNQEVHLIELDMTDDAK"
     gene            complement(4712..>5250)
                     /locus_tag="PLB46_09645"
     CDS             complement(4712..>5250)
                     /locus_tag="PLB46_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF76823.1"
                     /translation="AGIGLGFEFAKEIEVEGLSIIGSVDFLVNGLSKEITESFEDDLD
                     PSDKVTYLKYINVPVLIGAKYSILASNIGIFGKFNVGLNMLHITDFTVEYDAFNGTNT
                     LSFDNSLKLAYGIGAGIVLNEKISIGFDYLGLGSHPIDGKDTYEDDSFDSEVDISDYY
                     DTRDITVSGIKLHIGLIF"
BASE COUNT         1701 a          874 c          973 g         1702 t
ORIGIN      
        1 aaaacgatgc accggttcag gatgcacgat tattacctca ggcaaattat tacggcggcg
       61 atcttgcggg aattcaacaa aaaattgatg atggttattt cagcaattta ggtatcaatt
      121 cactttggat ttcaccggta tttcaaaatc cttattctgc ttatcaagaa tttccagaac
      181 cacatcgatg gtttagcgga tatcatggtt attggccaat ttcattaagt aaggtagata
      241 ccaggttggg aacaaacact gaatttaaac aattggtaag cggcgcacat aatagtaata
      301 tgagtgttta tttggattat gttgccaacc atattcacgc cgaacaccct ttatggaaat
      361 cgaatcccga atggttttca caactcgatt tacctgatgg cagaaaaaat ttgcgtttat
      421 gggatgagta tcgactaact acctggtttg aaccatatat gccgagcttt gatcatagca
      481 aaccgattgt tgcggaggct tgtgcagaca gtgccatgta ttggttaaaa gaatttaatc
      541 tcgacggatt tcgtcacgac gcaaccaaac atattgaaac agaattttgg cgcttgctca
      601 cgtataaaat aaaaacggaa attgaaattc cgcaaaacag aaatatttat caaattggcg
      661 aaacgtttgg ttctcgagaa ttaatcagct cttatttagg ctcgggatta ttaaatgctc
      721 aatttgattt tccattgtat ttcgatgcaa gaagtgtatt tgcaaataat gaagttgggt
      781 tcgatagatt aaataccagt ttacacgaaa cttttgatgc gtatggttac catcacgtaa
      841 tgggcaatat tacaggcaat cacgatatgg cccgatttat atcttatgcc agtggcgatt
      901 taaaatggga tgaaaacgat aaagaagcag gctggaatcg caatattacc gttaccgata
      961 caataggtta tgctaaactc aaaatgttac atgcgttcaa tatgaccatt ccgggaattc
     1021 cgattatata ctacggcgat gaaattggta tgccgggcgc caacgacccc gataatcgga
     1081 gaatgatgcg tttcgacaac cttaatccgt ttgaaaccga tgttaaaaat acggttacag
     1141 aactaaccaa gctacgcatc tcgcacatgg cgttgttata tggggatttt gaagtgctaa
     1201 tggttaacgc aacacaatat gtttacaaac gcacttacct caatgacgaa gtgattgttg
     1261 tttttaataa aagtccgcaa aaaactacta ttattgtaaa cgatacaata agtaaaaata
     1321 aataccagcc aaatttcggc aatataattg ttgtttccga taattcgatt gaagtaactt
     1381 tggcaccgta ttcgtttgaa ataattacac taaaaaataa ctaagcatac cacaatgaaa
     1441 aaaattgcct tcgtgctttt tgcactcgta ctcactcaac taacatttgc tcaggcacct
     1501 gaatggacaa aagataaaac catttacgaa gtaaatattc gtcaatatac acaggaagga
     1561 accttcgacg cttttgcaac acatttacca cgcctcgaaa aaatgggtgt tggtatttta
     1621 tggttaatgc cggttcagcc gattggtgaa ttaaaccgca agggaagttt gggaagttat
     1681 tattccattg ccaattacac ggcaactaat cccgaatttg gttccatgaa agattttaaa
     1741 aaattggtag atgaggcaca taaacgagac atgacaatta ttttagactg ggtggctaat
     1801 cataccagtt ttgatcatgt atggatgaaa tcacatcccg atttttatac gacagataaa
     1861 tcaggaaatg ttatcccacc ggtagcagac tggagtgatg tggcagattt aaattatgat
     1921 aataaagcca tgcgtgcggc catgattgcc gatatgaaat tttgggttga agaagcagat
     1981 atcgatggtt ttcgttgcga tgtcgcagga atggtacctg atgatttttg gatggaagcc
     2041 ttgacagaat taaaaaagat taaacccgat atttttttat tagccgaagc agaaggtcct
     2101 caatttcata ctgatggttt tcatatgaca tatggttggt ccaaacacca tatcatgaat
     2161 gaaattgcaa aaggaaaagc attagccgat agaatggata gtattattgt taaccataat
     2221 tcagtatatc caactggaag ttattacatg aacttcacca gcaaccacga tgaaaatagt
     2281 tggaacggaa ccgaatttga acgcatgggc gaaggtgcta aaacatttgg tgttatggct
     2341 gcaactatac cgggaatgtt tttattgtat accggtcagg aagttgcact cgatcgccgt
     2401 ttaaagtttt ttgataaaga ttcagtagtt tggctggaca acagaaactt cacccaattt
     2461 tataccactt taattcaatt aaaggcagat aatcctgcat tgtggaatgg tgcttatggt
     2521 ggtgattata aaagaattga tacacaacac gaaaaaattt atgcctattc cagaacattg
     2581 ggtgataata aagtagtagt tattttaaat ttatcttccg atgcacatga tgcggtatta
     2641 aataccgatt ccggaaaata taaaaatgta ttcacaggaa aaaaacaaaa actaaaggcg
     2701 ggcaaaaaaa ttactgtacc tgcttggggt tatgtggtgc ttaagcaata gctggttact
     2761 taactatctt gttgataaga acaagcatta aatttgacta ttatctactg taaattatta
     2821 atatgtttga taatactata ggaattttaa tacttgtatt ggcaacaatc ctttatttaa
     2881 ttgcatttta ttattccagg caggataaaa ttaaattagc agttttatta gttgtattag
     2941 ccggttttat cattagatgt tttgttgcca gtgatcctta tttgcatcct tgggacgaac
     3001 gttaccatgc gctggtggcg aaaaacatta tttcggaacc gctaaaacca accttatatg
     3061 aagaccctgt attgccgtat aattacaaac aatggcataa aaaccatatt tgggtgcata
     3121 aacaaccgat gccattgtgg atgatggcgg caagtatgcg tgttttcgga gtaaacgaat
     3181 ttgcattgcg aatgccaagt gtattgttgt cgacattagg catatggctt atttttttag
     3241 tcggcattta tttttatagt cataaagttg cttttatttc agcttttctt ttttcattaa
     3301 atggattgat tattgaatta accgggggta gggttgctac agaccattat gatttattgt
     3361 ttttgatttt tattttatca gctatttatt tcactacaaa atttgttaca actaaaagtg
     3421 tcatttttaa catattagcg ggattaatga ttgcatttgc tattttaacg aaatggctcc
     3481 ctgcattaat tgtgttacca atttggttga tattatctta ttcaagcaaa acattttcca
     3541 tagctgaaat ggcaaaacat tttgcactat tggttggcgt tattatcatt gtttcgtcac
     3601 catggcaaat ttatattgcc aatgtttttc ccgccgaaac agcatgggaa cagcactata
     3661 accgcctgca tatttttgaa atacttggtg gtagagatga gccgtggtgg ttttttcttg
     3721 agcgattgag aattaactat ggtgaacttg tttatattcc acttgtctat tttttatata
     3781 aattattcaa caaaacatca tctattaaat attatagttt ggccatttgg ataataatcc
     3841 cacttgtatt ttttacattg gtaaaaacaa aaatgcaagg ttatatttta ttcacttctc
     3901 cggctatatt tatgataacc gggtggttta ttttctacat gcaagaccaa aaccttcaag
     3961 gttggaaaaa gtatttgtcg agatttgtta ttgctttatt gctgttgctg cctgttcggt
     4021 atggtatcga aagatcgaag ctgcttgatc ccccttctaa agattacaac tgggttgcag
     4081 agctaagggc actagataag gatattcaac aaaattcggt attgtttaat tacaatatgc
     4141 caattgatgc aatgttctat acggagttta tggtatatca gcaattaccg gatgataaaa
     4201 caattttcga cttgcaagca agaggataca atgtatatgt tgcctatgag ggagaaatac
     4261 caagcgggtt tgaaaacaat caggaagttc acttaattga gttggatatg acagatgatg
     4321 ccaaataaac aattagtatg gcttcgataa ttattatttt ctgaattggg tgcatccccg
     4381 tagaatatgc attattttgt tatactagta acataaaaaa aatcaggtga ggtttataat
     4441 taaataaact cacctgattt tttcattcat actacaaaag gtatattcaa ttaataacct
     4501 ttttgggcgt aatatgtaaa ttgtttgaat gttttatcaa cctacgttcg attaaagttg
     4561 cttgttacta ttaccttaat gcaccaataa tttatgtttc acatttctat taatcaactc
     4621 aaatagttgt gcattttagc gtgatttagg tatttgcttt gataaatatt ctttaacaac
     4681 aataattaag aagctgacaa ataaatatca tttaaaagat caaaccgatg tgtaatttaa
     4741 taccggaaac cgtaatatcc cttgtatcat aataatcgct gatatctact tcgctgtcaa
     4801 aggaatcatc ttcataagta tctttaccat caattggatg agatcctaag cctaaatagt
     4861 caaagcctat agaaattttt tcatttagca ctattccagc gcctattcca tatgcaagtt
     4921 tcagggaatt atcaaaggat aaagtgtttg ttccattaaa tgcatcgtat tcaactgtga
     4981 agtcggtaat atgaagcata tttaaaccaa cgttaaattt accgaaaatg ccaatgttgg
     5041 aagctaaaat gctatactta gccccgatta aaacaggaac atttatatac tttaaatagg
     5101 ttactttgtc acttgggtca aggtcatctt caaagctttc tgttatttct ttagataacc
     5161 cgtttacaag aaagtcaacc gaaccgataa ttgataagcc ctccacttca atttctttgg
     5221 caaattcaaa tccaaggcca attcccgccc
//
LOCUS       DAORKW010000226         5239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_666_length_5239_cov_5.970293, whole genome shotgun sequence.
ACCESSION   DAORKW010000226 DAORKW010000000
VERSION     DAORKW010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_666_length_5239_cov_5.970293"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..352
                     /locus_tag="PLB46_09650"
     CDS             98..352
                     /locus_tag="PLB46_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76824.1"
                     /translation="MKLRIVIAFVAFFLLTSLSTELSAQCAMCKAAAEEGAKDGNLQS
                     ASLNTGIAYLLVMPYLAFMVVGYLWYRNYKKKKAEGTYIN"
     gene            complement(356..1057)
                     /locus_tag="PLB46_09655"
     CDS             complement(356..1057)
                     /locus_tag="PLB46_09655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF76825.1"
                     /translation="MNIVVTGATKGIGKAIALLFAKHGFNIAACARTQADLDTLKGEL
                     IAIHPSATIVTQVCDVSKKEQVIQFAATCKSAFNKIDVLINNAGVFIPGNIHNEPEGI
                     LEQLIDTNLYSAYHLTRALVPNMIKHQHGHIFNICSVASKNAYDAGGSYAISKFALYG
                     FSQNLRHELKQYKVRVTAVLPGAVLTDSWNGTQLPESRFINVDDVAKLIYQAWEVSDR
                     TVVEEILIRPMEGDI"
     gene            1111..3558
                     /gene="priA"
                     /locus_tag="PLB46_09660"
     CDS             1111..3558
                     /gene="priA"
                     /locus_tag="PLB46_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRF76826.1"
                     /translation="MNNLFADVILPFALEKNYTYAVPDEFGAALTPGMRVEVPFRNKS
                     YTGIVAKIHTNIPAGYQPKAILALPDHLHIVNKVQLEFWKWMAGYYMCSTGDVMNAAL
                     PAPYKLSSETIIVLKADVNIDAALLEDKEFLIAEALTIQKELKLKDIQLILQQKTIQP
                     TIKSLIEKGIVYVKEELTEVYTPKTEKFIVLNKKYTDEAALQQLFEQLQRHEKQLNIL
                     MVYYQHSQNNNKIKKSFLLRKSGASAAVLKTLIKNNIFEEIDEQVSRLQLYGDDQMDD
                     FVLNAEQERAINETRNFLKEKSTVLIHGVTGSGKTEVYIELIKEYIQSGKQVLYLLPE
                     IALTTQIISRLKKRFGNQIGVYHSKFNQNERIEIWQRVLNNEYRIILGARSSLFLPFN
                     DLGLIIIDEEHDYSYKQHEPAPRYHARDAAIYLAYLHNAKTVLGTATPCIETYFQAVH
                     GKFGLVKMLKRYADMAMPEIRIADVKEEEKNKTMHSHFTSALVGRMKYALDLKEQIIL
                     FQNRRGYAPFLICEACGWTPRCINCDVNLVYHKFSNELRCHYCNYTNNTYTVCPACGS
                     SRIIIKGFGTEKIDDELQYLFPEAKTGRLDLDTVKSKHGHEKILRAFESGDIDILTGT
                     QMVTKGLDFDNVNLVGILSADQILNFSDFRAAERAFQMITQVSGRAGRKNKKGLVMIQ
                     AIQVNHPVLHYVMQNDYAGFYTKEIAERQQFGYPPFMRLIRITLRHNKVETVNKTASW
                     LGSELKNQLGERVLGPAIPGIPRIRNKYLSEIMIKFPNNRVSGEYVKQIVKLEVEKLQ
                     LNPSHKRVEIVIDVDPM"
     gene            3579..4019
                     /locus_tag="PLB46_09665"
     CDS             3579..4019
                     /locus_tag="PLB46_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRF76827.1"
                     /translation="MLSKKAKYGLKAMFYLGKQYGKGPVLISDLAEEENIPKKFLELI
                     LLELKKNGLLHSKMGKGGGYSLSKSPDEITVGQVVRILDGPLAPIPCVSKTAYMKCAE
                     CDNEKTCEIRKIMMQVRDASAAILDHTSLLEAPKALFTVNRKSK"
     gene            4128..5171
                     /locus_tag="PLB46_09670"
     CDS             4128..5171
                     /locus_tag="PLB46_09670"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRF76828.1"
                     /translation="MKSTVLLLVVMLSLSVGMYAQSATQVETYNVKVSQKIELRGLTH
                     VRYQLFEDSTKFDAFDLRRARLDFRGDIAPKVGYRLHTELAGTPKILDATFVYKPYEW
                     LNVNVGQSKYPVCYDNLYSPWNLLTISRTQIDNSLSFRESDLYGNQNGRDIGLWLSGK
                     YSIGSEEAKRPLLDYTLGIYNGSGINVADNNQDKDISAALGISPIKDLWIFGRFLNGI
                     GRTTVQPTVDADRTRFGGNISYKYKSFIVEGEYLAASDESDSLALLERSGYYVTLGYT
                     PIKDKLQIIARLDNYDPNTATEDNITNKYILAASYFFTKNTRIQLEYNLVLEEAETQK
                     ENNLFAIQFQAAF"
BASE COUNT         1777 a          817 c         1049 g         1596 t
ORIGIN      
        1 ataacctggc ctttatggtt ttatgttgcc gtaacaggcg tggtggtata ttttatgatt
       61 gcaccctatt atccgcatta atgcttaaat ttgtactatg aaactccgta tcgtaattgc
      121 gtttgtggcg ttttttctgc taactagcct cagcacagaa ttgagtgcac aatgtgctat
      181 gtgtaaagcc gctgccgaag aaggtgctaa agatggcaac ctccaaagtg catcgttaaa
      241 taccggaata gcttatttat tggtgatgcc ttatttggca tttatggtag taggttacct
      301 ttggtataga aattacaaga aaaagaaagc cgaaggcact tatatcaact aagagttaaa
      361 tatcaccttc cataggtcgt attagtattt cttccacaac agttctgtct gaaacctccc
      421 aggcttggta aatgagtttg gcaacatcgt caacattaat aaatctggat tcaggtaatt
      481 gtgtgccgtt ccacgaatcg gttaaaactg ctccgggcaa tacagctgta actctaacct
      541 tatactgttt gagttcatgg cggaggtttt gagaaaaacc gtataatgca aacttcgaaa
      601 tggcataaga gccaccagcg tcataagcgt ttttactggc tacggaacaa atgttaaaaa
      661 tatgaccatg ttggtgttta atcatattgg gaactagggc acgtgtcaga tgataagcag
      721 aataaagatt agtatcgatg agctgctcca atatgccttc cggttcatta tgaatattgc
      781 cgggtataaa aacacctgca ttgttaatca ggacatcaat tttgttaaat gcagatttac
      841 aggttgctgc aaattgaatt acctgttcct tttttgatac atcacaaact tgtgtaacaa
      901 ttgtagcaga tgggtgtatt gctatgagtt cacccttaag tgtgtctaaa tcggcttgag
      961 tgcgagcgca cgcggcgata ttaaaaccgt gttttgcaaa taatagcgca attgctttgc
     1021 ctattccttt agtggcaccg gtaactacaa tgttcatcgt taagtgattt aatttaaata
     1081 gttttacaaa ataaaacaat ttaccccgca atgaataact tgtttgctga tgtgattttg
     1141 ccgtttgcgt tggaaaagaa ctacacctat gcagttccgg atgagtttgg tgcggcgctt
     1201 actccgggta tgcgtgtaga agtgcctttc agaaataaaa gttatacagg tattgttgct
     1261 aaaattcata caaacattcc cgccggttat cagccaaaag cgatattagc tttgcccgat
     1321 catttgcata ttgtaaataa ggttcaatta gaattttgga aatggatggc aggttattat
     1381 atgtgttcaa caggtgatgt gatgaatgct gcattacctg caccttacaa actcagcagc
     1441 gaaacaatta ttgtgttgaa ggcggatgta aatatagatg ctgctttatt ggaagataaa
     1501 gaatttttaa ttgcagaagc attaactatt cagaaagaac tgaagttaaa agatattcaa
     1561 cttatacttc aacaaaaaac aattcaaccc accataaaat cgctgataga aaagggaatt
     1621 gtttatgtaa aggaagagtt gacagaagta tatacaccaa aaactgaaaa gtttattgta
     1681 ttaaataaaa agtacaccga tgaagcggca ttacaacaac tgtttgaaca attgcaacgc
     1741 catgaaaagc agttaaatat tttaatggtg tattatcagc attcccaaaa taataataag
     1801 attaaaaaat catttttatt aagaaaatcg ggagcatcgg cagctgtgct taaaacgctt
     1861 ataaaaaata atatttttga agaaatagat gaacaggtca gcagattaca attgtatggt
     1921 gatgaccaga tggatgattt tgttttaaat gcagaacagg aaagagcaat taatgaaaca
     1981 aggaattttt taaaagaaaa aagtactgta ttaattcatg gtgttaccgg tagtggtaaa
     2041 acggaagtgt atattgagct cataaaagaa tatattcaat ctggcaaaca agtattgtat
     2101 ttgttgccgg aaattgcatt aaccacacaa attatttcac gacttaaaaa acgttttggc
     2161 aatcagattg gtgtttacca ttctaagttt aatcaaaacg aaagaattga aatttggcag
     2221 cgtgttttaa ataatgaata tagaattatt ttaggggcgc ggtcttcgtt gtttttgcct
     2281 tttaatgatt tgggtttgat aattattgat gaggagcatg attattcgta taaacaacac
     2341 gaacctgcac cgcgttatca tgcccgcgat gcagcaattt atctggctta tttacataac
     2401 gccaaaacag tattgggaac agcaacacca tgtattgaaa cttattttca ggcagtgcat
     2461 gggaaattcg gattggtgaa aatgctaaaa cgatatgccg atatggcaat gccggaaatc
     2521 cgtattgctg atgtaaagga agaagaaaaa aataaaacca tgcattcgca ttttacttct
     2581 gctttggtgg gaagaatgaa atatgcattg gatttaaaag aacaaattat tttgtttcaa
     2641 aatagaaggg gatatgcgcc atttctcatt tgtgaagctt gtgggtggac acccagatgt
     2701 attaattgtg atgtaaacct agtttaccat aagttcagta atgaattgag atgtcattat
     2761 tgtaattata caaataatac ttataccgta tgcccggcat gtggaagctc gcgaattata
     2821 attaaaggtt ttggaacaga aaaaattgat gatgaattgc aatatttatt tcctgaagca
     2881 aaaacaggca ggttagattt ggataccgta aaatcgaaac atgggcatga aaaaatatta
     2941 cgtgcatttg agtcgggcga tattgatatt ttaaccggta cacaaatggt tactaaggga
     3001 ctggattttg acaatgtaaa tctggttggc attttaagtg cggatcagat tttaaatttt
     3061 tctgatttca gagcagcaga aagagcattc caaatgataa cacaggtaag tgggagagcc
     3121 ggccggaaaa acaaaaaggg ccttgtaatg attcaggcca ttcaggtaaa tcatcctgta
     3181 ttacattatg tcatgcaaaa tgattatgcc ggattttaca ccaaagaaat tgccgagcgg
     3241 cagcaatttg gttatccgcc atttatgcgt ttaattagaa ttacgttgag acataataag
     3301 gttgaaactg taaacaaaac agcatcctgg ctggggtcag agctcaaaaa tcaattgggc
     3361 gaacgtgtgt tgggacctgc aattccggga attccgcgta ttcgtaataa atatttgtcg
     3421 gaaatcatga ttaaattccc aaacaacagg gtaagcggcg aatatgtgaa acaaattgtg
     3481 aaattagaag tggaaaagtt gcagctaaat ccatcccaca aacgggttga aattgtgata
     3541 gatgttgacc caatgtagta tctttgccgc ataaacctat gttatctaaa aaagccaaat
     3601 acggactaaa agctatgttt tacctgggta aacaatatgg caaaggcccg gtactcattt
     3661 ctgatttagc agaagaggaa aacataccca aaaagtttct cgaactgatt ttacttgagc
     3721 tgaaaaaaaa tggtttatta cacagcaaaa tgggcaaagg cggtgggtat agcctaagta
     3781 aatcgcctga tgaaattact gtaggacagg tagtgcgaat attagatggc cccttagctc
     3841 caataccatg cgttagtaaa accgcctata tgaaatgtgc agaatgtgat aacgaaaaaa
     3901 cctgtgagat taggaaaata atgatgcagg ttcgcgacgc atcagcagcc attttagacc
     3961 ataccagttt attggaggca cctaaagcct tgtttaccgt aaatcgaaaa tcaaaataaa
     4021 taccagcatt tacaagcatt tatatattaa aaaaataaat atgtataaat atattctact
     4081 aattcgatag aatatataga tatttgtgcg cacaaaaaat attatttatg aaatcaacag
     4141 ttttactttt agtagttatg ttaagtttgt cggttgggat gtatgctcag tcggctacac
     4201 aggtggagac ctataatgta aaagtttcgc agaaaattga attacgcgga ttaactcatg
     4261 ttcgttatca gttgtttgaa gattcaacca agtttgatgc atttgattta cgtcgtgctc
     4321 gtttagattt tagaggcgat attgcaccta aagttggata tcgcttacat actgaacttg
     4381 caggtacacc taaaattttg gatgccactt ttgtttacaa accatatgaa tggttaaacg
     4441 taaacgtagg gcaaagtaaa tatccggttt gttatgataa cttatactca ccttggaatt
     4501 tattaacgat aagccgaaca caaatagata atagtttatc gtttcgtgaa agtgatttgt
     4561 atggcaacca aaacggtcgt gatatcggtt tatggttaag tggaaaatat agtattggca
     4621 gtgaagaagc taagcgtcca ttattagact atacccttgg tatatataat ggttcaggaa
     4681 taaacgtagc agacaataat caggataaag atatttctgc agcattgggt atatcaccaa
     4741 tcaaagattt atggattttt ggtcgatttt taaacggtat aggcagaaca accgtacaac
     4801 caacagtaga tgcagacaga actcgttttg gtggaaatat ttcctataaa tataaaagtt
     4861 ttattgttga aggagaatat ttagctgctt cagatgaaag cgatagctta gcattgctgg
     4921 aacgcagtgg ttattatgta actttaggtt atacaccaat taaagataaa ttacaaatca
     4981 ttgcccgatt agataattat gatcctaata ccgctactga agacaatatt accaataaat
     5041 atattctggc tgccagttat ttctttacta aaaatacacg tattcaatta gaatataatt
     5101 tagtattaga agaagctgag acacaaaaag aaaataattt atttgcaata caattccagg
     5161 cagcgtttta aattgcctgt tttaaatggt tattctattg gaatagccat ttatttttat
     5221 ttaatatact ataaaatct
//
LOCUS       DAORKW010000227         5230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_671_length_5230_cov_7.440773, whole genome shotgun sequence.
ACCESSION   DAORKW010000227 DAORKW010000000
VERSION     DAORKW010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_671_length_5230_cov_7.440773"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..3028
                     /locus_tag="PLB46_09675"
     CDS             239..3028
                     /locus_tag="PLB46_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76829.1"
                     /translation="MKNNNTFTTHNKIGLLSILVVSLALAYASSNTNSIFSLKNDTTE
                     NNLLINPDSIPETFDTTSSFEVGDTIALNLAGLDTLFVLYDDTTLAGSDTLNDTLALR
                     LFDVNKDITITPGVITKTVAPGLFGIHIEGMFTPKHLPQDTGNVNFPNAWNWMADLKP
                     KVLRFPGGASSRWMHLLPYKDEVAPFGVLDPIKGYGYDIKEIIRYYDVTDGSIEADNP
                     AFMDSIIDDLTDTICNDCNTWMDIGYTPDFVKLYEKYLEQETNIPANQQQQYIDQFIA
                     LIDTIQKRGDYTVDVIVDLNIISESASQCKQIIDYLQNPDPKIDGGNGVTSVHVVGVE
                     MGNECNLGWATDIMGFYGFDDYWNLINGKGKGDDPIGELTPEFEAWLDDFSEYVFSED
                     YFDNHDFIAAFKSEPTFNMKVGIPAANLKNNDSTKFAFRIMEDLSTSWNEDLVAHYAD
                     SLVFGSQVRYLFDAVILHTYYDAKTNWDTLATANLCHATYPNSGMPTCVPDSICGNWT
                     DDRWQFSFTDTRLKAAYEAVIGLGSNGFGNFKQFIRSRYAESYDQQKIDLLFAGTQPW
                     KKELWTTEWNMKDKNTDYENNDPEQYILSSFCNSFEHGWITQEWFMNNIKQNYASGYR
                     QGFHTYSTFHAYGGGSFYAMLLQSDKADRINHLDESGLPDTLSDPPTGQNLFLRKTVY
                     YTFEMLSEIQKKNLKYLQSNFTAYAHNPNVQPTVFIDKPGKKLYIYYSNMKSETQSYV
                     VKKGYLLGLYPGSTALAYGEALLYNIDALQLYSNSGNSYLYKLNICYNNIDQLHPFEI
                     QGINGPTANVPECSGLADGAICVTVPANSFGYIEIPFYVSAREGIILSDDQVELFPNP
                     TANAFRITCTIPEELLNEFVVDVFDLQGKLVMHTTTSQNTEIDVSNLPSDIYIIKISN
                     REQSFAVNKKLIKIE"
     gene            3028..3924
                     /locus_tag="PLB46_09680"
     CDS             3028..3924
                     /locus_tag="PLB46_09680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76830.1"
                     /translation="MENTKFSLKQYSGFAGVLLLMHNNTHAQAIYTDIDPDVVLQFHG
                     DVEYIDMENDGTNDFFIFKKSSYFTNSEYSWYRFNRIFKAGPNFDPENEIAGDFHTNG
                     AGGGTTYFPYALLEGDLISIDLSFQYGHYQLMGIGFFQVDETPWDWNYYIGSWRLKID
                     SAYLGVRFLGADDCKHYGWIRCSVGDSLQTLTVHDFAYESKCNTPIIAGDTIGDTSTV
                     EIAEINNWEAIIYTFNKNVYINLHNQSEINVFIFNINGQEIYNRSSSQKNIVIDMTGE
                     PIGTYIIKLTTSDKQFTKIIDL"
     gene            4217..4777
                     /locus_tag="PLB46_09685"
     CDS             4217..4777
                     /locus_tag="PLB46_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76831.1"
                     /translation="MKAKLFFCFMLIGLAFSTHAQSWFTPGTVYQFESKSVSNPQEVI
                     TLRVVYDTQGNATVTKSTRYAGSQDKQKTCSFGPPAELSISTNQGAPGITTYTYTTKN
                     KYWMIPFDTYTSAAMPSTETTITCTCTSNGEATCDVSTQTDSSGACSTCVPGTGCMTC
                     KMTVSNSTFINIGGVLIIKANNITLQ"
     gene            complement(5067..5225)
                     /locus_tag="PLB46_09690"
     CDS             complement(5067..5225)
                     /locus_tag="PLB46_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HRF76832.1"
                     /translation="MNNLVSRTVMNTKPITVEYSITEYGKSLSPLIDELAKWGGEYRK
                     SVYKNGKN"
BASE COUNT         1716 a          961 c          955 g         1598 t
ORIGIN      
        1 aaattcaagt tcagtttttc aaatcaactt tagtgctggg ttgacagttt tgtgctccga
       61 aatcccgcac gaacgccaag cgcaaaacgt tataaccaat ggcttacaat ggaattcacg
      121 atattacaag cataaaccta cccagacgaa tttcgtttgc ttgtttaaat aagcgcagac
      181 gaaggtgttt ttttatacat ttttaaaaat tatttatcta acatttaata ttatttgtat
      241 gaaaaacaat aacacattta ctacccataa taaaattgga cttttgtcaa ttttagttgt
      301 ttctttagcc ttagcgtacg caagcagtaa tactaattca atttttagtt taaaaaacga
      361 tacaacagaa aataatttac taattaaccc ggattcaatc cctgaaactt ttgatacgac
      421 aagtagtttt gaagttggtg acacaattgc acttaatttg gcaggcttag ataccctatt
      481 tgttttgtat gacgacacaa cgcttgccgg aagcgataca ctaaacgaca ccttggcact
      541 tcgattgttt gatgtaaata aagacatcac tataacaccg ggcgtcatta ccaaaactgt
      601 tgctcccgga ttatttggta ttcatattga aggcatgttt actcctaaac atttaccgca
      661 agatacaggc aatgtaaatt ttcctaatgc atggaattgg atggcagacc ttaaaccaaa
      721 agtattacgt tttccgggag gcgccagcag cagatggatg catttattac cttacaaaga
      781 tgaagttgca ccctttggtg tgctcgatcc gattaaaggt tatgggtatg atattaaaga
      841 aataatacgg tattatgatg ttactgacgg aagtattgag gctgataatc cggcttttat
      901 ggattcaata atcgatgacc ttacagatac tatttgcaac gattgcaaca catggatgga
      961 cattggctat acacctgatt ttgttaaatt atatgaaaaa tacctcgaac aagaaaccaa
     1021 cattccagcc aaccaacaac aacaatacat cgatcaattt attgctttaa tcgataccat
     1081 acaaaaacga ggggattata ccgtagatgt aattgtcgat ttaaatataa taagtgaatc
     1141 agcatcacaa tgtaaacaga taattgacta tttacagaac cccgacccta aaattgatgg
     1201 cggtaatggt gtaaccagtg tgcacgttgt tggtgtagaa atgggtaatg aatgtaatct
     1261 tggttgggca acagacatta tggggtttta tggctttgat gattattgga atttaataaa
     1321 cgggaaagga aaaggggatg accctatcgg ggaactcaca ccggaatttg aagcttggtt
     1381 agacgatttt tcagaatatg tatttagcga agattatttc gacaaccatg attttattgc
     1441 agcctttaaa tcggaaccaa catttaatat gaaagtggga attccggcgg ctaatttgaa
     1501 aaacaatgat tccacaaaat ttgcgtttcg aataatggaa gatttatcca cttcttggaa
     1561 tgaagattta gttgcccatt atgcagatag tcttgtattt ggctcacagg ttcgatattt
     1621 gtttgatgcc gttattttac acacctatta cgatgctaaa acaaattggg atactttggc
     1681 tacagccaat ttgtgccatg ccacatatcc taattccggt atgccgacat gtgtaccgga
     1741 tagtatatgt ggtaattgga cagacgaccg ctggcaattt agttttaccg acacgcgttt
     1801 aaaagccgct tatgaagcag taatcggttt aggttcaaat ggtttcggca actttaaaca
     1861 attcattcgt tcacggtatg cagaatctta cgaccaacaa aaaatagatc tgctgtttgc
     1921 aggtactcaa ccttggaaaa aggaattgtg gacaacagaa tggaatatga aagataaaaa
     1981 tacggattat gaaaataatg acccggaaca atatatcctt tcctccttct gtaattcatt
     2041 tgagcatggc tggattacac aagaatggtt tatgaacaat attaaacaaa attatgcatc
     2101 aggctaccgc caaggatttc acacctattc cacgtttcat gcttatggtg gcggctcatt
     2161 ttacgccatg ttgctgcaat ctgacaaagc tgataggatt aatcatttag atgaatctgg
     2221 attgccggat acattatctg atcctccaac cggtcaaaat ttattcctta gaaagactgt
     2281 ttactatact tttgaaatgt tgagtgaaat acaaaagaaa aacttaaaat atttgcaaag
     2341 taattttacc gcatacgcac acaatcctaa tgtgcagcca actgttttta ttgacaaacc
     2401 cggtaaaaag ctgtacattt actattcaaa catgaaatct gaaactcaaa gttatgtagt
     2461 aaaaaaaggc tatctccttg gtctgtatcc gggctcaacg gcgcttgcct acggagaggc
     2521 cttgttatac aatattgatg ctctacagtt gtattccaat agtggaaata gctatttata
     2581 taagttgaat atttgttaca acaatatcga ccaattgcat ccttttgaaa ttcaaggcat
     2641 taatggtcct acggctaatg tgcccgaatg ttccggttta gcagatggag caatatgcgt
     2701 taccgttcct gcaaattcat tcggatatat tgaaattcca ttttacgtat ccgctcgcga
     2761 aggcataatt ttatccgacg accaagtaga attattccca aacccaactg caaatgcatt
     2821 tcgcattacc tgtacaattc ccgaggagct gcttaatgaa tttgtggtag acgtgttcga
     2881 tttgcaaggt aaattagtga tgcataccac aacaagccaa aatacagaga ttgatgtaag
     2941 taatttacct tccgatattt atataattaa aattagtaat cgtgagcaaa gttttgcggt
     3001 taataaaaag ttaataaaaa ttgaatgatg gaaaacacaa aatttagttt aaaacaatac
     3061 tccggttttg ccggggtatt gttacttatg cataataata cacatgcaca agctatctat
     3121 acggacattg accccgatgt tgttttacaa ttccatgggg atgttgaata tattgatatg
     3181 gagaacgacg gaacaaatga tttttttata tttaaaaaga gtagctattt taccaactct
     3241 gaatatagct ggtaccggtt taaccggata tttaaggctg ggcccaattt tgacccggaa
     3301 aatgaaattg cgggtgattt tcatactaat ggtgcaggtg gcggtactac ctattttcct
     3361 tatgcattat tggaaggcga tttaataagt attgatttaa gttttcaata tggtcattat
     3421 caattaatgg gtattgggtt tttccaagtt gatgaaacac cctgggattg gaattattat
     3481 attggtagct ggagactaaa aattgattca gcctatttgg gggttcgatt tttaggggcc
     3541 gatgattgca agcattatgg ttggatccgc tgttcagtag gtgattcact ccaaacacta
     3601 accgtacatg actttgccta tgaatcaaaa tgtaacacgc ccattattgc cggcgataca
     3661 atcggcgaca cgtctacggt tgaaattgct gaaataaata attgggaagc aataatttat
     3721 acttttaaca aaaatgtgta tattaattta cataaccaaa gcgaaataaa tgtatttatt
     3781 tttaatataa atggacagga aatttataac agaagttcat ctcaaaaaaa catagtgatt
     3841 gacatgacgg gggagccaat tggaacttat ataattaaac taacgacaag tgacaaacaa
     3901 tttacaaaaa taattgacct ataagcattt tcattagcgg taagtaagcc actggttata
     3961 acaaaacctt tgcaaaatgg cttggtgact agtaaccatg aaaatatatc gccaaatagt
     4021 aattttaatg ccaatgaatt atatcgttta gttgccgcca cttcgcaaag cttctaaacg
     4081 ttatgcgcaa actatgatca gatattagtg acgcttataa tgcagagaag agtataaatt
     4141 atctcccatt tggatactaa actgaaaata attacgttac ttttatatat attttaaccc
     4201 atttaatttt atcaaaatga aagcaaaatt gtttttctgc tttatgctaa ttggattagc
     4261 atttagcact catgcccaaa gctggtttac ccctggtaca gtgtatcaat ttgaatctaa
     4321 atcagtgtct aatccacaag aagtgattac tttacgagtg gtgtatgata ctcagggaaa
     4381 tgcaaccgtt acaaaatcca ccagatacgc tggttcgcaa gacaaacaaa aaacatgttc
     4441 gttcggtcca ccggcagaat tatcaatttc tactaaccaa ggtgcaccgg ggattacaac
     4501 ttatacctat accacaaaga ataaatattg gatgatccct ttcgatacat atacctctgc
     4561 cgcaatgcca agtactgaaa caacaattac ttgcacctgc acctccaacg gtgaggccac
     4621 ttgcgacgtt tcaacacaga cagattcatc aggtgcatgt tcaacttgtg ttccaggaac
     4681 gggttgtatg acatgtaaga tgacagtttc aaattcaaca tttataaaca ttggaggcgt
     4741 attaatcatc aaagcaaata atattacatt gcaataatcc gcaaccataa ttgaaaagcc
     4801 tttgattcat gatttgttga gtcaaaggct tttcaatttg aatcatatac tatcaaatac
     4861 tcaaccctac caaaccgtca gcgcataaca gcacatttgc aataggcggg gcttcgtgct
     4921 ccgcagacag ttttgcggta gccgaaagtt tcgtgctccg cataaagttc agtggtaaaa
     4981 atcccgccca tcgcaaatct gccaaacgtt agcggcaagt atagcgaccc atctgacatt
     5041 tccacacggt tcagcataac accaaattag ttctttccat ttttatagac agactttcga
     5101 tattcacctc cccattttgc caactcatca attagaggcg aaagactctt accatattca
     5161 gttattgaat actcaacagt tatcggtttt gtgttcatta ccgtccgact gaccaaatta
     5221 ttcatttcca
//
LOCUS       DAORKW010000228         5212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_674_length_5212_cov_6.171805, whole genome shotgun sequence.
ACCESSION   DAORKW010000228 DAORKW010000000
VERSION     DAORKW010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_674_length_5212_cov_6.171805"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLB46_09695"
     CDS             complement(<1..255)
                     /locus_tag="PLB46_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76833.1"
                     /translation="MVSTILISIAGFFALIIIIGIFIPSKYRFIRTTTIDADRRKVFN
                     TIDDLTTWKEWSAWSKNKDPKIDITYGEKTSGNGASMQWKG"
     gene            complement(342..617)
                     /locus_tag="PLB46_09700"
     CDS             complement(342..617)
                     /locus_tag="PLB46_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76834.1"
                     /translation="MKIFTTRYFVIVTIFAIAIFIVTSIISNFLLKTGPIVIQLGYPY
                     AYYLRYGGYMSGQHASNVNYLLIDFLFAWTAVAIITFFYRKKLITKK"
     gene            complement(649..1044)
                     /locus_tag="PLB46_09705"
     CDS             complement(649..1044)
                     /locus_tag="PLB46_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76835.1"
                     /translation="MKTVGLLFFVTVFFSSCEYLNFNNKISAEQIQAEEERPFIFIDI
                     TGNVEPDRITCQITNNAAATTYKNIDVTIHYFMDTRELIRSEHHVLEATVGPKSTVKH
                     IIAINPPPIHYSIVYYSVKNAEVVTEGGI"
     gene            complement(1059..3887)
                     /gene="uvrA"
                     /locus_tag="PLB46_09710"
     CDS             complement(1059..3887)
                     /gene="uvrA"
                     /locus_tag="PLB46_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRF76836.1"
                     /translation="MKEKKNHQNGHSDAIFIKGAREHNLKNIDVTIPRNQLVVVTGVS
                     GSGKSSLTFDTLYAEGQRRYVESLSSYARQFLTRLDKPDVDYIRGIAPAIALDQKVTT
                     RTTRSTVGTLTEIYDYMRLLFARVGRTYSPVSGREVKKDEVSDVVDYIGSLPQDTKVY
                     ILVELESDKKRKPEEIARVLQQKGFTRFYCNDTIVKMDDVIDNPKLIPAASKLFLLID
                     RIVVQKEIAEHHTTPAINEELQQRIADSIDIAFYEGHGACLIHAENLPDRTFSNNFEA
                     DGVVFEVPSPHFFNQNNPYGACKRCEGFGTVIGVDHDLVFPDKNLSIYQGAVACWKGE
                     KMRAWLDELIKVATKFDFPIHKPVHQLSKEQYKLLWTGNEYFSGLDDFFKMLEENAYK
                     IQYRVMLARYRGKTVCPECLGSRIRQDANYVKINNHSISDLIDMPVEDLALFIRNIEF
                     SDFENKVASRILLEMNNRLGYMLDLGLGYLTLNRKSNTLSGGETQRINLTRTLGSNLT
                     SSLYILDEPSVGLHPRDTERLVKVLKSLRDLGNTVVVVEHEEEVIKNADHIIDIGPEA
                     GIHGGKVISSGNFDFIYNDKNSLTGNYLSETRSIPLPKHRRKSANKIKIKQARMHNLK
                     NIDVTIPLHSLVAVSGVSGSGKTTLIKHILHPELMKMLDDTSDTAYISKSLEGDLRTI
                     TQVELVDQDPIGKSSRSNPVTYVKAYDEIRELYARQPLSKMRGYKAGFFSFNVDGGRC
                     ETCKGEGEIIVEMQFLADVHLTCEECGGKRFKEEILEVQYKDKNIADLLGLSVEEALE
                     FLKDEKEIIYKLQPLFDVGLGYVKLGQSSSTLSGGEAQRVKLASFLGKGRSREHVLFI
                     FDEPTTGLHFHDINKLLSAFHALIECGHSIIVIEHNIEVLKCADYIIDLGPEGGEKGG
                     NLVYEGSPEGLPKVKESYTGKYLKHKFN"
     gene            4061..4639
                     /locus_tag="PLB46_09715"
     CDS             4061..4639
                     /locus_tag="PLB46_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF76837.1"
                     /translation="MQSRTLPDDQLVQLYLSGEESALGELLNRHKDRIYTYIYLFVRD
                     TYTAEDLMQETFIKVIDKLRSGKYKEQNKFLPWLSRVAYNLCIDYYRREKRLPKVTTR
                     DGFDIFSVLKFSEPGAEGKIIENEFSTKIRKILEHLPEEQREVVILRHYMDMNFREIA
                     ELTGVNINTALGRMRYALINLRKLMEAKNISI"
     gene            4708..4947
                     /locus_tag="PLB46_09720"
     CDS             4708..4947
                     /locus_tag="PLB46_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76838.1"
                     /translation="MTKNITQDNLIRYIYSETTAEETSLIEEALRSDWELKELHDNLL
                     ETKSELGKVKVSPSQKVIDNIMRYSKDNAPMEHFQ"
BASE COUNT         1627 a         1058 c          914 g         1613 t
ORIGIN      
        1 acctttccat tgcatggagg cgccgttgcc ggatgttttt tctccgtagg taatgtcaat
       61 ttttgggtct ttgtttttgc tccatgcact ccattccttc caggtagtca aatcgtcaat
      121 agtattaaaa accttgcgcc tgtccgcatc aattgtggtg gtgcgaataa accggtattt
      181 cgacggaata aaaatgccga taataatgat tagtgcaaaa aaacctgcta ttgaaattaa
      241 aatagtactt accataacgt ttttcgaagt aataaaagta gttattattt gggaagaaat
      301 acaacagtat gcgcattgta tactatcttc ctcaactata tttatttttt agtgattaat
      361 tttttgcgat aaaaaaatgt aataatggcc acggctgtcc aggcaaaaag aaaatctata
      421 agcagataat ttacgttaga tgcatgttgc ccggacatat aaccgccata acgcaaatag
      481 taggcataag gatatccaag ctgaataaca attggccctg tttttaaaag aaaattcgaa
      541 ataatggaag tcacaataaa aatagcaatg gcaaaaatcg taacaataac aaaatagcgt
      601 gtggtaaata ttttcatgag aaaagtttaa ttattaccct aactaaattt aaattccacc
      661 ctcagtaaca acctctgcat ttttaaccga ataatataca atactataat gtattggtgg
      721 tggattaata gctataatat gttttacagt cgacttggga ccaacagttg cttctaaaac
      781 atgatgttcg gaacggataa gctcacgcgt atccataaaa taatgaatgg taacatcaat
      841 gtttttataa gtagttgctg ctgcattatt ggttatctga caagtaattc tgtccggttc
      901 tacattgccg gtaatgtcga taaaaataaa aggacgttcc tcttcagcct gaatttgctc
      961 cgctgaaatt ttattattaa aatttaaata ttcgcaggaa ctgaaaaata cagtaacaaa
     1021 aaacaataaa ccaacagttt tcataacgtt taaaataatt agttaaactt gtgttttaaa
     1081 tatttacctg tataactttc tttcactttg ggcaaacctt caggcgaacc ttcatacact
     1141 aaattaccac ctttttctcc gccctcagga ccaaggtcga taatgtaatc tgcacatttt
     1201 aatacttcaa tattatgttc aataacaata atactgtgtc cacactcaat taatgcatga
     1261 aaagcggata ataatttatt gatatcgtga aaatgtaacc ctgttgtcgg ctcgtcgaaa
     1321 ataaataaaa catgttcgcg acttctgcct ttgcctaaaa aactggcgag ttttacacgt
     1381 tgtgcttcgc caccggataa tgtgctgctg ctttggccta attttacata acccaatcct
     1441 acatcaaata gaggttgcaa tttgtaaata atttcttttt catcttttaa aaattccaaa
     1501 gcctcctcaa cgcttaatcc caataaatcg gcaatatttt tatccttgta ttgaacttct
     1561 aaaatttctt ctttaaaacg ttttcctcca cattcttcgc aggttaaatg tacatcggct
     1621 aaaaattgca tctccacaat aatttcaccc tcacctttac aggtctcgca tctgcctcca
     1681 tcaacattaa agctgaaaaa tcctgctttg taaccgcgca ttttcgacaa tggctggcgc
     1741 gcatataatt cacgaatttc atcgtatgct tttacatagg taacagggtt ggagcggctc
     1801 gatttgccga ttggatcctg gtcgacgagc tcaacctgtg taatcgtgcg taaatcacct
     1861 tccagcgatt tcgaaatata tgcagtgtcg ctggtgtcat ccagcatttt cattaactca
     1921 ggatgtaaaa tatgtttaat caatgttgtt ttacccgaac cactcacacc ggataccgca
     1981 actaaactat gcagcggaat agtaacatcg atatttttta aattatgcat ccgtgcctgt
     2041 ttaattttta ttttattggc acttttcctt ctatgtttag gtaacggaat acttcttgtt
     2101 tcagataaat aattacctgt taaactattt ttatcgttat aaataaaatc aaaattaccg
     2161 ctgctgataa ctttaccacc atgaatacct gcttcaggtc caatgtcaat aatgtggtca
     2221 gcatttttaa ttacttcttc ttcgtgctcc acaacaacta cggtattacc taaatcgcgc
     2281 aaacttttta atactttcac taaacgttcg gtatcgcgtg gatgtaatcc cacactcggc
     2341 tcatctaaaa tatataacga tgaagttaaa ttactgccca atgttcgcgt taaattaatg
     2401 cgttgtgttt caccaccact taatgtatta ctttttctgt ttaaggttaa atagcccaat
     2461 cctaaatcga gcatataacc taaacgatta ttcatttcca acaaaatacg cgatgccact
     2521 ttattttcga agtcgctgaa ttcaatattg cgaataaaca acgcaagatc ttcaaccggc
     2581 atatcaatta aatcggaaat agaatgatta tttattttca cataattggc atcctggcga
     2641 atacgagaac ctaaacattc cgggcaaacc gttttgccac ggtagcgggc aagcataacg
     2701 cgatattgaa ttttgtaggc gttttcttcc agcattttaa aaaaatcatc aagtcccgaa
     2761 aaatattcat tgccggtcca taataattta tattgctctt tgctgagctg atgaacaggt
     2821 ttgtgaatcg ggaaatcaaa tttggtagcg actttaatta attcatctaa ccaggctctc
     2881 attttttcac ctttccaaca agcaacagca ccctgataaa tagataaatt tttatccgga
     2941 aaaaccaggt catgatcaac accaattaca gttccaaaac cttcacatcg tttgcatgcg
     3001 ccgtatggat tattctgatt aaaaaagtgt ggactcggca cttcaaatac tacaccatca
     3061 gcttcaaagt tgttgctaaa agtgcggtcg ggtaaatttt ctgcatgaat taaacatgcg
     3121 ccgtgacctt cataaaatgc aatatcaata gagtcagcaa ttcgttgttg taattcttca
     3181 tttatagcgg gagtagtgtg gtgttcggca atttcttttt gcaccacaat acggtcgatt
     3241 aataaaaata atttcgaagc agccggtatt aatttcggat tatcaattac atcatccatt
     3301 ttcacaatgg tatcgttgca ataaaatcgc gtaaatcctt tttgttgtaa aacgcgtgca
     3361 atttcttcag gcttgcgttt tttatcgctc tcgagttcta ccagaatata aactttggta
     3421 tcctgaggca atgaaccaat ataatctacc acatcgctca cctcatcttt tttcacctca
     3481 cggccagaaa ctggggaata ggtgcgtcca acacgggcaa aaagtaggcg catgtaatca
     3541 taaatttcgg tcaaagtgcc cacagtgctt cgtgtggtgc gggtggtaac cttctggtcg
     3601 agggctatgg caggggcaat gccgcgtata tagtccacat cgggtttatc gaggcgggta
     3661 aggaattgtc tggcatagga actcaggctt tccacgtaac ggcgctgccc ttcggcatat
     3721 aaagtatcga aagtaaggct cgatttgccc gaaccgctta ctccagtaac cactacaagc
     3781 tgattgcgag gtatggttac gtcgatgttt ttcaggttat gttccctggc tccttttata
     3841 aatattgcgt cagaatgacc attctgatgg ttttttttct ctttcaaagg tgttttattg
     3901 aaactttttc aaaaatatca cgtaaatgtt gtccattccg tttggaattt ccaaaaaaat
     3961 gtatatttgc attaactaaa cacaaaacta ctgcctataa ggttaacatc tacgctaaaa
     4021 attattattc attttatcgt ttaacgcaca accttattgt atgcagtcca gaactcttcc
     4081 tgatgaccaa ttggtccagc tgtacttgtc cggtgaagaa tcggcactag gtgagctcct
     4141 caatcgccac aaagatagga tttacaccta catttattta ttcgtacgtg atacgtatac
     4201 tgctgaagac ctcatgcagg aaacgtttat caaggtaatt gataaactca gaagtggcaa
     4261 gtataaagaa caaaacaagt ttttgccttg gttgtcgcgc gtggcgtata acctgtgtat
     4321 cgactactat cgccgcgaga agcgtttgcc gaaggtgacc acccgcgatg ggtttgatat
     4381 attcagtgtg ctcaaatttt cggagccggg tgctgaagga aaaattattg aaaacgaatt
     4441 ttcgaccaaa atccgcaaaa ttcttgagca tttgcctgag gaacagcgcg aagtggtaat
     4501 tctgagacac tacatggaca tgaatttccg cgaaattgct gagttaaccg gggtaaatat
     4561 caataccgcc cttggccgta tgcgttatgc gctaattaat ttgagaaaat tgatggaggc
     4621 aaaaaatatt tcaatttgag ataataataa gttgagcaat gccgttttag ttgcataatt
     4681 tttatggtta aacctaaaac atattgcatg actaaaaaca ttactcaaga caatctcatt
     4741 cgttacatct attcagaaac aaccgccgag gaaaccagtt taatagaaga ggcgctccgg
     4801 agcgattggg aactgaaaga attacatgac aacctgctcg aaaccaaatc ggaactcggc
     4861 aaggtgaaag tcagtccttc acaaaaagtg atcgacaaca tcatgagata cagcaaagac
     4921 aacgctccga tggagcactt tcagtaaaaa actcaaaacc ctgaccaaag cgtcggggtt
     4981 tttttatgcc tgttaatgaa gtaaaataaa atatttgggc gttgccgcgc aatatttaat
     5041 cccactccga attacctttt cgagcgcggc cgggttttac gctctaactc ctaacgcgat
     5101 acaatgcagc cttgattttt tggttctttt gtatcaagac aaaagaacac taaatcacat
     5161 tcgaagctat tattcaaaaa cacctctaat ccccccaatc ttcaaatact aa
//
LOCUS       DAORKW010000229         5199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_683_length_5199_cov_4.711314, whole genome shotgun sequence.
ACCESSION   DAORKW010000229 DAORKW010000000
VERSION     DAORKW010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_683_length_5199_cov_4.711314"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..798
                     /locus_tag="PLB46_09725"
     CDS             385..798
                     /locus_tag="PLB46_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76839.1"
                     /translation="MRKTTTLAMALLPAATLFFGCDKGSDEKGTPDVTGPEILYYEPD
                     PNDVFAVNDTIFLQADVTDDSELQDFSVKLIKGTDTILVWPEEVVIFGNIKSYHLVDY
                     TIDTYHINSPAIILYEASDKKDNVTKVEVPIELTM"
     gene            complement(860..1711)
                     /locus_tag="PLB46_09730"
     CDS             complement(860..1711)
                     /locus_tag="PLB46_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76840.1"
                     /translation="MKQFSTVFFTFLLSLCISAVANAQIPMGAYTAEQTAYITAEDPT
                     MTDGRNFKNLPVTMKKGEGAMFYMRAILFEPQIYIIDNAQINWRQGDAVIGSDGITSS
                     ILIFIADKDTSFNVIFTTEMAGSGGEFVYGMRQLDANQMSVPTENDFCGRLTYMVNHW
                     QCMWYLMDGVQDVYGKTFNFSLIPDNSFELDDFFIASEDVYAPETSEEAKAKGTELIE
                     KIKKCLDMNAWEIKEDYYLSDIDGVEYTTTYFNVKGASNKDFMMSFAVIVEIDPTGLN
                     YVVIEFY"
     gene            1796..2245
                     /locus_tag="PLB46_09735"
     CDS             1796..2245
                     /locus_tag="PLB46_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76841.1"
                     /translation="MAQQKKTSKTWSEHAIHAPETDYLYIENSRIAGAGRGLFTAITI
                     YKEEIIAVYTGETITEQEAVKRALAGNDDYFMNLHDGNTLDCMSSNCFAKYANDVEGT
                     RLKANAFIGMNELNEVCLIANKTIHAGSEIYCNYGKKYWKKRQHKLS"
     gene            complement(2249..3472)
                     /locus_tag="PLB46_09740"
     CDS             complement(2249..3472)
                     /locus_tag="PLB46_09740"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRF76842.1"
                     /translation="MDFTTFGFEQELMEGMMSMGFKAPTPIQEQAIPIILSNKDIIAC
                     AQTGTGKTAAYLLPVINKIIKRGHGSINTLIIVPTRELAVQIDEAIRAFTYFTNLSSI
                     AVYGGNDGVMYEQEKRSFREGVDIIVGTPGRLLSHISQGYLKFDTLKHLILDEADRML
                     DMGFSDDISRIVSYLPENRQSLLFSATMPGKIRAIAKKILKDPDQINIALSKPAEGVT
                     QGVYILHDAQKMPLLRIILNGRDIKSILIFSSTKEKVKQLEMELKRLKYNVSAIHSDL
                     EQIQRKAVLRSFSNRELQILVATDILSRGIDIDNIGLVINYDVPGDAEDYIHRVGRTA
                     RADTKGEAITLVGPNDQRKLMQIEELIEGEINRLQVPEELGEVPAYEPKLRRNSGNFN
                     RGGKKTFHRRSGPRR"
     gene            complement(3962..4567)
                     /locus_tag="PLB46_09745"
     CDS             complement(3962..4567)
                     /locus_tag="PLB46_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76843.1"
                     /translation="MQYDYPTIARKLNQCWPDFIFNQSNLEACLERETITAGDYFPKF
                     EITEFQVALVISGAFRLYYIAQNKEVNIQFLFENDLLIDLNNHTQVNYHQFSLQAIEN
                     TEILVIDVAKLFERKGELFIQSGIDRFITQTLIQITTEHLTTNLYLSGEARYSKLFEA
                     KPEYFVRLPLYHIASYLGMEKESLSRIRRTIIEKRKKIRAA"
     gene            complement(4701..5117)
                     /locus_tag="PLB46_09750"
     CDS             complement(4701..5117)
                     /locus_tag="PLB46_09750"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76844.1"
                     /translation="MYIYDSADETTTHLHPSLDPDMPSSIYNYFTIAPNGDLWFGHND
                     IGISALKTTATVGIADLTSNTIHVYPNPTSDVLTIDYETTTEPRYDIVDLQGKLMLSA
                     HTNNINVQSLAQGLYIAKVTDMVTLQTYTTTFSVAK"
BASE COUNT         1734 a          949 c          851 g         1665 t
ORIGIN      
        1 aatggtaaaa ttgttaaatc tgtaaccagc acgacttcaa ctagtgaaac tttgtcactg
       61 aacattgaaa accttgctgc gggtatctat acagttacca ctacttctgc taacgcaata
      121 agtgcaacaa aattaattgt tgagtaatca ttaagttttt aaatacagta aaaaagtcag
      181 cctcgtgctg acttttttta tacctgaaca agggattttc tatgtcacat ttgtccaaaa
      241 tttgtagccg ttaatcaaac cccttgaaaa ccctatggtg ggctaattca tcatcaccga
      301 tttaagtttt aattaaaatt ttaattatag ggttgtgtta ttttttgtca taattagcta
      361 aattagacct gtcaaacaca cactatgagg aaaactacta ctttggcaat ggcactactg
      421 ccggctgcaa cactattttt tggttgtgat aaaggttccg atgaaaaagg cacacccgat
      481 gtaaccggac ccgaaattct ttattacgaa cccgacccga atgatgtatt tgctgtaaac
      541 gacaccattt ttttacaggc agatgtaaca gatgattcag aattacaaga tttttcagtc
      601 aaattaataa agggaacaga tactattcta gtttggcctg aagaggtggt gatttttggt
      661 aatattaaaa gttaccatct cgtagattat acaattgata catatcatat aaactcgcct
      721 gcaatcattt tatatgaagc tagtgataaa aaagataatg ttacaaaagt tgaagtgcca
      781 attgaattga caatgtgaat aataaaaaaa gaggctgtcc ggataaacag cctctttaac
      841 aataaattaa atgtaagcat taataaaatt ctattactac atagtttaac ccggttggat
      901 caatttctac aattactgca aaagacatca taaaatcttt atttgatgca ccttttacat
      961 taaaataggt ggttgtgtat tccactccat caatatctga aagataatag tcttctttta
     1021 tttcccaagc attcatgtcc aagcattttt taattttttc aattaattca gtgcctttag
     1081 ccttagcctc ttcgctagtt tccggggcat acacatcttc acttgcgata aaaaaatcat
     1141 ctaattcaaa ggaattgtcc gggattaatg aaaaattaaa tgttttacca taaacatcct
     1201 gcacaccatc cataagatac cacatacatt gccaatgatt aaccatataa gttaatctgc
     1261 cacaaaaatc attctctgtg ggtactgaca tttgattcgc atctaactga cgcataccat
     1321 acacaaattc gccacccgat cctgccattt cagtagtaaa tatgacatta aagctggtat
     1381 ctttatccgc aatgaatatt aaaatagaag aagtaatgcc gtcacttcca attacagcat
     1441 caccttgtct ccaattaatc tgtgcattgt caataatata aatttgtggt tcaaataata
     1501 ttgcgcgcat ataaaacatt gcaccttcac cctttttcat agttactggc aaatttttaa
     1561 agtttctgcc atcagtcatg gttgggtcct cagcagtgat ataggccgtt tgttctgcag
     1621 tatatgcgcc cattggaatt tgtgcattag ctacagcaga tatacataaa cttaacagaa
     1681 aagtgaaaaa aacagtcgaa aattgtttca ttttgtgcat ttagataaca aagataaaaa
     1741 tatgaataat ttcctataat gattattgta actttgaagt aggtaataaa tatcattggc
     1801 acagcaaaaa aaaacatcca aaacctggtc agagcatgcc attcatgcgc ctgaaaccga
     1861 ttatttatat atcgaaaatt cgcgtattgc aggtgcaggt cgaggtttat ttactgcaat
     1921 cactatttat aaggaagaaa taattgccgt ttataccggt gaaacgatca ctgaacagga
     1981 agctgtaaaa cgagctttag caggaaatga tgattacttc atgaatttac acgatggcaa
     2041 tacactcgac tgtatgtcca gtaattgttt tgcaaaatat gcgaatgatg tggaaggcac
     2101 cagattaaaa gcaaatgcat ttattggcat gaatgagttg aatgaagtat gtttaatcgc
     2161 caacaaaacc attcatgcag gttctgaaat atattgtaac tacgggaaaa aatattggaa
     2221 aaaacgccag cacaaattgt cttaggtatt accttctcgg accacttctg cgatgaaatg
     2281 tttttttacc gccccgatta aaatttcctg agttccttct caatttaggt tcataagccg
     2341 gcacttctcc taattcttca ggcacttgca gtctgttaat ttccccttca atcagctctt
     2401 caatttgcat cagtttgcgc tgatcatttg gacccaccaa tgtgattgct tcacctttag
     2461 tatcggcacg tgcggtgcgg cctacgcgat gtatataatc ttcagcatca ccgggaacat
     2521 cataattaat taccaacccg atattatcga tatctatacc gcgggataat atgtctgtag
     2581 cgaccaatat ttgtaattct ctgttactaa aactacgcaa cactgctttg cgttgaattt
     2641 gttctaaatc ggaatggatg gctgatacat tatattttaa tcgtttcaac tccatctcca
     2701 attgtttcac cttttctttt gttgatgaaa atatgagtat gctttttata tcgcgaccgt
     2761 ttaatataat tctcagtaaa ggcatttttt gcgcatcgtg caaaatataa acaccttgcg
     2821 taactccttc tgcaggtttt gataaggcaa tattaatttg gtcggggtct tttaatattt
     2881 ttttagcaat agctctaatt tttcccggca tggttgctga gaataataat gattgtctgt
     2941 tttcgggtaa ataagaaaca atacgactaa tatcatcact aaatcccata tccaacatcc
     3001 tgtctgcttc atccaaaata agatgtttca acgtatcaaa ttttaaatac ccttgtgaaa
     3061 tatgtgataa taatcttccg ggtgtgccaa caataatatc aacaccttca cgaaatgaac
     3121 gtttttcctg ttcatacatt acaccatcat taccaccata tacagcaata gaactcaaat
     3181 tggtaaaata agtaaatgcc cttattgcct catctatctg aacggctaat tcgcgggtag
     3241 gaacaattat taatgtattg atggagccat gtcctctttt aataatttta ttgattacag
     3301 gtaataaata cgcagctgtt tttcctgttc ccgtttgcgc acaggctata atatctttat
     3361 tgctgagtat tatcggaatg gcctgttcct gaattggcgt gggggctttg aaacccatcg
     3421 acatcatccc ctccattaat tcctgttcaa atccaaatgt cgtaaaatcc atgtagtgtt
     3481 tttataatat acatacaaat ttacgcattt tataactaat gagctaaaag gcaatagcat
     3541 agcgcatttc agggacgtaa gcatgcaaat tctaagcggc ttgaaaacaa gctcgatgca
     3601 taaaaacacg agctatacta agtgatttat gttttcaata taaatattga aaacataaat
     3661 caatgcacca atctgttcag tcgcaaaata attttattac aggagagatg ttatttaaat
     3721 gaataatatt ccaaagccaa cgcaatttac ctgttgtgga gaaaaaaggt tgagcaagtc
     3781 aatttaagga gaaataatat cattagcatc atctaaacgc aacaccttaa aaacaataaa
     3841 tttatcagga actagcaaaa catattaaat tgcagtacaa gttatacaat aaatacgatc
     3901 taaattgttg gggataaatg gttacagtat atcagttaat aactcaacca tctattgatg
     3961 tttacgctgc acgtattttt tttctttttt caataatggt gcgacgaata cgactcaaac
     4021 tttctttttc catacctaga tatgaggcaa tatgatataa aggtaaacgc acaaaatact
     4081 caggtttagc ctcaaacaat tttgaatacc gagcttcacc actcaaatac aaattggtgg
     4141 tgagatgttc ggtggtaatt tgaatgaggg tttgtgtaat aaatctgtca atgccggatt
     4201 gtataaataa ttcgcctttt ctttcaaata attttgccac atcaataact aatatttcag
     4261 tgttttcaat agcctgtaaa ctaaattgat gataattaac ctgggtatga ttgttaaggt
     4321 caatcagcaa atcgttttca aacaggaatt gaatattcac ctctttgttt tgagcaatat
     4381 aatataaacg aaatgctcct gaaataacta acgcgacttg aaattctgta atttcaaact
     4441 ttggaaaata atcgccggct gtaattgttt cccgctccaa acatgcttct aaattggatt
     4501 gattaaaaat aaaatccggc cagcattggt ttaacttgcg cgctatagtt ggataatcat
     4561 attgcataaa ccatcattta aatatgaata aataaacaat aaaaaaagtc gccttaaaca
     4621 ggtagagtat taaatgctgt tttacaaaag ctgtaagtgc aaataactgt ttaaggcgac
     4681 cagcccctta aagggataat ttatttagct acactaaacg tagttgtata agtttgtaag
     4741 gttaccatat ctgtaacttt tgcgatatat aaaccctgtg ctaatgattg cacattgata
     4801 ttgttagtat gcgcagataa catgagttta ccttgcaaat ccactatatc ataacgtggt
     4861 tcggtggtgg tttcataatc aatagtcagc acatcacttg tcggattcgg ataaacatga
     4921 atagtgttag atgttaaatc agcaatacca acagtagcag tcgtcttcaa cgcgctgata
     4981 ccaatatcgt tatgaccaaa ccataaatca ccattaggag caatagtgaa atagttatag
     5041 atgcttgatg gcatatcagg atcaagagaa ggatgtaaat gggtggtagt ttcatcagcg
     5101 ctatcataaa tatacatgcc cgcatcagtt ccatttatac caaaataggt cttggttcct
     5161 ttaaattgaa tacttgtaac aaaatcaaag tcttaaccg
//
LOCUS       DAORKW010000230         5190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_685_length_5190_cov_5.496787, whole genome shotgun sequence.
ACCESSION   DAORKW010000230 DAORKW010000000
VERSION     DAORKW010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_685_length_5190_cov_5.496787"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1321
                     /locus_tag="PLB46_09755"
     CDS             <1..>1321
                     /locus_tag="PLB46_09755"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HRF76845.1"
                     /translation="VSFADVDGDGYTSDVDCNDANAAINPGASESCNGIDDNCDGNID
                     EGVQTTYYADMDGDTYGDAGNSTMACSMPEGYVADATDCNDGDAAVNPGATEVCNGID
                     DNCDGNNDEGLTFTTYYADADGDTYGDAGSTVSTCDGAPEGYVSDATDCNDADAAVNP
                     GATEVCNGIDDNCDGNIDEGVQNTYYADMDGDSYGDAGSSTMACSAPEGYVTDATDCN
                     DADGAVNPGAAEVCNGIDDNCDGNTDEGLTFITYYADADGDTYGDAASSATTCDGAPE
                     GYVADATDCNDADAAVNPGATEICNGIDDNCDGNIDEGVQNTYYADMDGDTYGDAGSS
                     TMACSAPEGYVADATDCNDADGAVNPGATEVCNGIDDNCDGNTDEGVQNTYYADADGD
                     TYGDAGSTTIACSTPEGYVSDASDCNDADAAVNPGATEVCNGIDDNCD"
     assembly_gap    1322..1421
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1422..2987
                     /locus_tag="PLB46_09760"
     CDS             <1422..2987
                     /locus_tag="PLB46_09760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF76846.1"
                     /translation="GNTDEGVQNTYYADADGDTYGDAGSTTMACSTPEGYVSDATDCN
                     DADGAVNPGATEVCNGIDDNCDGNIDEGLTFTTYYADADGDTYGDAASTVTTCDGAPE
                     GYVSDATDCNDADGAVNLGATEVCNGIDDNCDGNIDEGVQNTYYADADGDTYGDAGST
                     TMACSAPEGYVADATDCNDADGAVNPGATEVCNGIDDNCDGNTDEGVLITFYADADGD
                     THGDAASSVDACSAPEGYVASNDDCNDASATVYPGAPEICNNGIDEDCDGGIDVISTI
                     AAGGPTTFCLGNSVTLSSTTVGTGYGYQWMKNGSNIAGANSSSLNVTLQGNYKCTVTK
                     GDCSSTSSVITVSVNLNPNATIVALDGTDLCGKSYVRLKANNGTGLTYQWYMDGVIID
                     GATGNVYYASLPGSYYVVVTNAAGCSKQAAAVTVISSCRIAEEGAVAFNVIPNPSNGN
                     FQLNVNLGNGLNGNAIISVVNIAGQKVAQLNGEIANGEMSANVSIPQVAGVYMVVVEA
                     NGEVFSQQIMITE"
     gene            3276..3806
                     /locus_tag="PLB46_09765"
     CDS             3276..3806
                     /locus_tag="PLB46_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76847.1"
                     /translation="MELFWQIVGYIIPALVVFLTTYVVIRAFLDREQSMREMEFRQAN
                     LRESLPIRLQAYERLTLFLERIALNNLLPRVRKQEMTVAEFRTALINNIRMEYEHNLS
                     QQVYVSAETWGMIRAAKEETISVINRNAMTIMPELPGIELHKKIIGELAESEDDAITL
                     KVLVQLKKEVMLLYGK"
     gene            3951..4673
                     /locus_tag="PLB46_09770"
     CDS             3951..4673
                     /locus_tag="PLB46_09770"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HRF76848.1"
                     /translation="MLSIFFITTSCSYYYAESSHQSSQVRVMEITPDSAANALILPYK
                     SSLDGEMNSIVAEIGNEMRKSKPESALGNFICDVIVKQGEIVTGNNIDFGVYNYGGIR
                     QDVIAEGPITKGKIFELLPFENFGAIVTLDGPSTLLLIQKIIDEEGWPVSGELQIVIK
                     NNLPEKILINGAPFDITKSYVVVMNDYMAGGGDNSAFLTGQKVDVLGVTIRAMMLDYL
                     AAETAAGRKIYSKTEGRIVYAD"
     gene            4663..>5190
                     /locus_tag="PLB46_09775"
     CDS             4663..>5190
                     /locus_tag="PLB46_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF76849.1"
                     /translation="MQTRRIFLKKIALGSAVAGLGGIPLQSFAAGSVDKLVILHSNDV
                     HSRIDPFPDNDKKFPGMGGMANKAAIIKKIRSEEDNVLLLDAGDIFQGTPYFNFYGGE
                     LEMKLMSEMQYDAGTMGNHDFDAGIDGFVKQLPHTTFPILVANYDFTGTALENKIEQY
                     KIFQKKNIKVGVFGLG"
BASE COUNT         1575 a          806 c         1170 g         1539 t
ORIGIN      
        1 agtatcgttt gctgatgttg atggcgatgg ctatacttct gatgttgatt gtaatgatgc
       61 aaatgcagca attaatccag gtgcttcgga atcatgcaat ggcattgacg ataactgcga
      121 tggcaatatt gatgaaggag ttcaaaccac ttattatgct gatatggatg gcgacactta
      181 tggtgatgcc ggaaattcaa ctatggcttg ttcaatgcct gaaggatatg tagcggatgc
      241 aactgattgt aatgatggag atgctgctgt aaatcccggt gctacagaag tttgtaatgg
      301 tatagatgat aattgtgatg gcaataacga tgaaggttta actttcacta cttattatgc
      361 agatgctgat ggcgacactt atggtgatgc cggatctaca gttagtacat gtgatggtgc
      421 acctgaagga tatgtttcag atgcaactga ttgcaacgat gcagatgctg cagtaaatcc
      481 tggcgcaact gaagtttgta acggtataga cgataattgc gatggcaaca tcgacgaagg
      541 tgttcaaaat acttattatg cagatatgga cggcgactcc tatggtgatg caggttcatc
      601 aacaatggca tgttcagctc cggaaggata tgtaactgat gcaacagatt gtaatgatgc
      661 agatggtgca gtaaatccag gtgcagctga agtatgtaat ggtattgacg ataattgtga
      721 tggcaatacc gacgaaggtt taacttttat aacttattat gcagatgctg atggcgacac
      781 ttatggtgat gcagcttctt cagcaactac ttgcgatggc gctcctgaag gatatgtagc
      841 cgatgcaaca gattgtaatg atgcagatgc tgcagtaaat ccgggtgcta ctgaaatttg
      901 taatggtatc gacgacaatt gcgatggcaa tatcgatgaa ggcgttcaaa atacttatta
      961 tgctgatatg gatggcgaca cttatggtga tgcaggttca tctactatgg catgttctgc
     1021 tccggaagga tatgtagctg atgcaacaga ttgtaatgat gcagatggtg cagtaaatcc
     1081 tggtgcgaca gaagtttgta acggaatcga tgataattgc gatggcaata cagacgaagg
     1141 tgttcaaaat acttattatg ctgatgctga tggcgacact tacggtgatg caggttcaac
     1201 tacaattgca tgttcaactc ctgaaggata tgtgtcagat gcatcagatt gtaatgatgc
     1261 tgatgctgca gtaaatcctg gtgcgacaga agtttgtaac ggaatcgatg ataattgcga
     1321 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggtaataca gacgaaggtg
     1441 ttcaaaatac atattatgct gatgctgatg gcgacactta tggtgatgca ggttcaacaa
     1501 caatggcatg ttcaactcct gaaggatatg tatcagatgc aacagattgt aacgatgcag
     1561 atggtgccgt aaatccgggt gctacagaag tttgtaatgg catcgacgac aattgcgatg
     1621 gcaatatcga cgaaggttta acttttacta cttattatgc agatgctgat ggcgatactt
     1681 atggcgacgc agcttctaca gtaactacat gcgacggtgc tcctgaagga tatgtatcag
     1741 atgcaacaga ttgtaatgat gcagacggtg cagtaaatct aggcgctact gaagtttgta
     1801 atggtataga tgataattgt gatggcaaca tcgatgaagg tgttcaaaat acatattatg
     1861 ctgatgctga tggcgacact tatggtgatg caggttcaac aacaatggca tgttcagctc
     1921 ctgaaggata tgtagctgat gcaacagatt gtaacgatgc agacggtgca gtaaatccgg
     1981 gcgctactga agtttgtaac ggtattgatg ataattgcga tggcaataca gatgaaggtg
     2041 tattaattac cttctatgct gatgccgatg gtgacactca tggtgatgct gcttcttcag
     2101 tggatgcatg ttcagcacct gaaggttatg tagccagcaa cgacgattgt aatgatgctt
     2161 ctgcaactgt ttatccgggt gcacctgaaa tttgtaataa cggaattgat gaagattgtg
     2221 atggtggtat cgatgtaatt tctacaatag ctgccggtgg tcctactaca ttctgtttag
     2281 gtaacagcgt tactttaagc agcacaactg taggcacagg atatggctat cagtggatga
     2341 aaaacggttc taacattgcc ggtgcaaata gttcgagctt aaatgtaaca ttacaaggta
     2401 actataaatg tactgttaca aaaggcgatt gctcaagcac ttcaagtgta ataactgttt
     2461 ctgttaacct taacccaaat gcaactatag tagctttaga tggcactgac ctttgcggaa
     2521 aatcttatgt aagattaaaa gcaaacaatg gaacaggttt aacttatcag tggtatatgg
     2581 atggtgtaat tatagatggt gctacaggta acgtttatta tgcatcatta cctggcagct
     2641 actatgttgt agttaccaat gcggcaggtt gcagtaaaca agcagcggct gtaacagtta
     2701 tttccagctg tcgtattgct gaagaaggtg cagtagcatt taatgtaata ccaaaccctt
     2761 ctaatggtaa tttccaactt aatgttaatt tgggtaatgg tttgaatggt aatgccatta
     2821 ttagtgtagt taacattgca ggtcaaaaag tagctcaatt aaatggtgaa atcgctaatg
     2881 gcgaaatgag tgccaatgta agtatccctc aggttgctgg ggtttacatg gtagttgttg
     2941 aagctaatgg tgaagtattt agccaacaaa ttatgattac tgagtaagat tagattttaa
     3001 ataaggaggg ctgtcgtaac cgacagccct ttttttatgc ctgtaaaggc catgatattc
     3061 cggctaatta taaattaaat ggttattttt agcgtatcgg gaataaagat ttacccttaa
     3121 ttacccttaa gatgtataat ttttcaaatg tttccccgtt cttaagctaa tgtaaaacct
     3181 caggtaagaa ttttagtttt aaaagaaatt tttacttcgg gtattggttt taatattctt
     3241 aattttattt ttgcaaccct cctaaaacga gatacatgga attattttgg caaattgtag
     3301 gatacattat ccccgcatta gtagtttttt taaccaccta tgtggtaatc agggcttttt
     3361 tggaccgtga acagagtatg cgtgaaatgg aattcaggca agctaatttg cgtgaatcct
     3421 tgccaattcg tttgcaggcg tatgaacgcc ttaccttgtt tttagagcgt atagcactta
     3481 ataatttatt gccgcgtgtg cgcaagcagg aaatgacagt tgcggagttc cgaacggcac
     3541 ttattaataa tatacggatg gaatatgagc acaacctaag tcagcaggtt tatgtgtctg
     3601 ctgaaacctg gggaatgata cgtgcagcca aagaggaaac catcagtgta ataaacagaa
     3661 atgcgatgac gattatgccc gaattgcccg gaatagagtt acataaaaaa attataggtg
     3721 aacttgccga aagtgaagat gatgccatta cccttaaagt gttggttcaa cttaaaaaag
     3781 aagtgatgct gttatacggc aaataaattc gccttacgtt ttcccttaca gatatttatt
     3841 tttttataat gttctcttga cctgaggata tagttacaac cactgtgcag cacttaccat
     3901 ttatatatca accattttgt cgtgggaaaa atcggctatt tagcatttta ttgctaagta
     3961 tttttttcat aactacttca tgtagttatt attatgccga atcatcacat cagtcatcgc
     4021 aggtaagggt tatggaaatt actcctgact cagcagctaa cgcacttatt ttaccttata
     4081 agtcatcgct tgatggagaa atgaattcca ttgttgccga aataggcaat gagatgcgca
     4141 agtcaaaacc cgaatcagca ctcggtaatt ttatatgtga tgtcatagta aaacaaggcg
     4201 aaatagttac tggcaataat attgattttg gcgtatataa ttatggcgga attcgtcagg
     4261 atgtaattgc cgaaggtcct attacaaaag gtaaaatttt tgaattatta ccatttgaaa
     4321 attttggtgc aatagtaaca ctcgatggtc cgtcaacttt attattaatt caaaaaataa
     4381 ttgatgaaga aggttggccg gtttcaggcg aattacaaat tgtaattaaa aataatctgc
     4441 cggaaaaaat tttaattaac ggagcaccat ttgatattac aaaaagttat gtggtggtaa
     4501 tgaatgatta tatggccggt ggaggagata attctgcatt tttaaccgga caaaaagtgg
     4561 atgtattagg cgtaacaatt cgcgcaatga tgctcgatta tttagcggca gaaacagcag
     4621 ccggaagaaa aatatacagt aaaacagaag ggaggattgt atatgcagac tagaagaata
     4681 tttctcaaaa aaatagcctt aggtagtgct gtagcaggat tgggcggcat tcctttacaa
     4741 tcgtttgcgg ccggtagtgt agataaatta gtgatattac attctaacga tgtgcatagc
     4801 aggatagatc catttcctga taatgataaa aaatttcccg gcatgggagg tatggcaaat
     4861 aaggctgcta tcataaaaaa aattcgcagt gaagaggata atgttttatt attagatgca
     4921 ggtgatattt ttcagggcac accctatttt aatttttatg gtggtgaact ggaaatgaaa
     4981 cttatgagtg aaatgcaata tgatgccggc acaatgggta atcatgattt tgatgcaggg
     5041 atagatggtt ttgtaaaaca attgcctcat acaacatttc ctattttggt agccaactac
     5101 gattttacag gaactgcttt agaaaataaa attgaacaat acaaaatttt tcagaaaaaa
     5161 aatattaaag taggcgtatt tggtttgggt
//
LOCUS       DAORKW010000231         5164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_696_length_5164_cov_4.938540, whole genome shotgun sequence.
ACCESSION   DAORKW010000231 DAORKW010000000
VERSION     DAORKW010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_696_length_5164_cov_4.938540"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1167)
                     /locus_tag="PLB46_09780"
     CDS             complement(205..1167)
                     /locus_tag="PLB46_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76850.1"
                     /translation="MKILLTLFLLVTVLFNSFSQGIPIPTTNTSWMFTNSSSDDGGNY
                     VYRGYILSTANDTIINDINYTNLNLTQNWEILNFFGYFTDDTNYDLGHIAAFRQDNGR
                     VYIYEYDVITDQLESGGGMFPFDFPIPNDTDFLLYDFNLEVGDTLFYLGYPASVVYSI
                     EDTLLADGLEHKKINFNGDDPGYQSWIEGVGSTLGFLPSHLPQTQVFHNAYQLDCFSS
                     NGVYLYGEDHCELVMVSDVENVSTTIIEVNPTLFHNQLSVLSLNSQFENYINVCNIEG
                     KSIFAANVMETEMRINTTNWISGVYFLTIYNARSNYKVFKIIKM"
     gene            1768..3033
                     /locus_tag="PLB46_09785"
     CDS             1768..3033
                     /locus_tag="PLB46_09785"
                     /inference="COORDINATES: protein motif:HMM:NF021537.2"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HRF76851.1"
                     /translation="MKPSFFVLLFFFVAQCFGAPLKINTWNSQISVSGNQQIVMPVTV
                     KKGELFQFYVMQDGLDVELLLITNKKDTVGYKDTDNGTFGHEVLFYEALKPEQLTLYI
                     KKLEGDYNPDSGAVSFYVKRFTNKEKDERTLLLKQLAEENKQHVLTADIDHFWMAFDK
                     LVNCKNYYDSISTIQNYYFDLATNGFLQFIKVRDLYAPEYLQKMKVEYAFYAEVRENT
                     YVTKEALPLIETVFDSLKIIYPNFKPFQVCFAIGALRTGGTTTGEFILIGTELTTTGE
                     KSKIPQRIKGIIAHEAIHTQQKYELKQGAITCHQLDECLDEGVANFIGELITGTTNYG
                     ETDTYGYAHEAELWNEFKSTLCASNTDNWLYNGDNSNNRPADLGYFIGYRIAKTYYQK
                     SANKRQAVIDLIEIDDPLSLLAKSGYDGK"
     gene            complement(3047..3475)
                     /locus_tag="PLB46_09790"
     CDS             complement(3047..3475)
                     /locus_tag="PLB46_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF76852.1"
                     /translation="MAILVFNTTINASKEKVWDVLWDDASYRKWTTVFHEGSHAVSDW
                     QEGGSILFLGPNGDGMFSMIVKKDDFHTMIFKHLGEIKNGERIPKQWGASTESYFMDE
                     KDGVTTLKVELNMEAEEWFETYFNSTFPKALEVVKKLAEE"
     gene            complement(3708..4160)
                     /locus_tag="PLB46_09795"
     CDS             complement(3708..4160)
                     /locus_tag="PLB46_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRF76853.1"
                     /translation="MKIQKHFLAFLLLLITLKGYAQSDSTQSDIAQITATLMDYIEGT
                     ANGEPERLRRAFHPDFNLYTVTQEDILRIRSGEEYIQGVKEGEKSDRIGRIIAIDYEN
                     NAAMAKAEIIIPGWRIFTDYFLLLKYEGSWKIVQKSYTWTAYPTAVNK"
     gene            complement(4198..4686)
                     /locus_tag="PLB46_09800"
     CDS             complement(4198..4686)
                     /locus_tag="PLB46_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRF76854.1"
                     /translation="MTLFCFLFLPINIWGQQLCTPNEIRTEIIINTTTTETWQLLTNF
                     NDYANWHPYIVKIDGEVSLKEKIKVTTIDTDSTTDQFSAFILEWEPDKQLAWGGSLGF
                     LFSAKHYFIIERINDTQILFIQGEYWHGIFGKNYGKKIYLDTYRKFIAMNEKMKAILE
                     SN"
     gene            complement(4877..4962)
                     /locus_tag="PLB46_09805"
     tRNA            complement(4877..4962)
                     /locus_tag="PLB46_09805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4926..4928),aa:Leu,seq:taa)
BASE COUNT         1692 a          960 c          844 g         1668 t
ORIGIN      
        1 acatgaatca agtccaggac atttttggtg attagcaaat ttgataatta gcaaattagc
       61 taatgagttt accccaagcc caaagtcaac aataatcctt tacatttaag atctcattag
      121 cttccgccgc ggcggacaca ttaatggctg tttcattggt acattggtac atcggtacat
      181 tggtacatta gctaaccagc agatttacat tttaattatt ttaaacacct tataattact
      241 gcgcgcatta taaattgtta aaaagtaaac accactaatc caattagttg tattaatcct
      301 catttcagtt tccataacgt tggcagcgaa aatagatttc ccttcaatat tgcatacatt
      361 aatgtagttt tcaaattgac tatttaaact taaaacagac aattgattat gaaacaatgt
      421 aggatttact tcaatgattg ttgtgcttac attttcaaca tcagacacca ttactaattc
      481 acaatggtcc tcaccatata aataaacacc atttgagcta aaacaatcca attgataggc
      541 attatgaaat acctgagttt ggggtaaatg tgatggcaaa aatcctagcg tacttccaac
      601 tccttctatc cagctctgat agcccgggtc atcaccatta aaatttattt ttttgtgctc
      661 taatccatca gctaacaaag tatcttcaat gctataaact acactagccg gataccctaa
      721 gtaaaaaagc gtatcaccta cttccagatt aaaatcatat aataaaaaat ctgtgtcatt
      781 aggtatcggg aagtcaaatg ggaacatacc gccgcctgat tcgagctgat cggttatcac
      841 atcatattca tatatataaa cacgcccgtt atcttgtctg aaagccgcaa tatgaccaag
      901 atcataatta gtgtcatcag taaaatatcc gaaaaaattt aatatttccc agttttgtgt
      961 aaggttgagg ttagtataat taatgtcatt tataatagta tcattagcgg tactcaatat
     1021 atagccacgg taaacataat tacctccatc gtcgctgcta ctattagtaa acatccagct
     1081 tgtgttagtt gttggtatag gaataccctg actaaaacta ttgaacaaca ctgttactaa
     1141 caaaaaaagt gttagaagga ttttcatggc taatcattta tataataaat ataataacat
     1201 tttgaaaaca ttccaaatta atataattta tgacctaata atgatatact gaagatgtat
     1261 taaccatttc attagtgtta aagaaaatga taaattaaat ggctcgtcgc aaaatcaact
     1321 ccacactcca caccaaagaa gtcgaagact tcatgttgtc gtggagtctt cgactccaga
     1381 cgccaccccg gactcctgtc cgcaatcgta cattattatt cgaagctatc accttcaatt
     1441 aaaattcaat tttaatacaa cacaaaactt ccaacatcca ataaaaacaa ataaaagaat
     1501 attgaataag cagatactgt tgaaataaat attgataaag ccgattaacc tccacatttt
     1561 tttcgccggt ctggagaccg gggtggcgtc tggagtcacg gaatccacga caacttggtg
     1621 tctgcgacgc aaagggtggg ttagtgttgg gtattgtaat tattaatcaa aacttcaacc
     1681 attctcgttt tggtgacaat attttcattt tatattttcc ttaacattca actttaccta
     1741 atttttttat ttttgtttac cactattatg aaaccatcat tttttgtcct cctcttcttc
     1801 tttgttgctc aatgttttgg agcaccgttg aaaatcaata cctggaacag ccaaatttcg
     1861 gtaagcggca atcaacaaat tgtaatgcct gtaactgtta aaaaaggcga gttatttcaa
     1921 ttttatgtca tgcaagatgg gctagatgta gaactattgc tcattacaaa taaaaaggac
     1981 acagtcggtt ataaagacac cgataatggt actttcggac atgaggtatt attttatgaa
     2041 gcgcttaaac ctgagcaact tacattatat ataaaaaaac tcgaaggaga ttataatccc
     2101 gattcaggtg ctgtaagttt ttatgtaaaa agatttacca ataaagaaaa agacgaaaga
     2161 acattattac taaagcaact tgcagaagaa aataaacaac atgttttaac cgctgatatc
     2221 gatcattttt ggatggcatt tgacaaattg gtgaattgta aaaattatta tgacagtatc
     2281 tctaccatcc aaaattatta tttcgattta gcaacaaatg gatttttgca gtttattaaa
     2341 gttcgtgatt tatatgcgcc ggaatattta cagaaaatga aagttgagta tgcattctat
     2401 gcagaagttc gagaaaatac ttacgtgaca aaggaagcat taccacttat tgaaactgta
     2461 tttgacagcc tgaaaataat ttatccaaac tttaaacctt ttcaggtatg ttttgcaatt
     2521 ggcgcattac gtacaggtgg aacaacaacc ggtgaattta ttttaattgg taccgaactg
     2581 accaccaccg gtgaaaaatc caaaatcccg caacgtatta aaggtattat agcccacgaa
     2641 gcaattcaca cgcaacaaaa gtatgaatta aaacaaggcg ctattacttg tcatcagtta
     2701 gatgaatgtc tcgatgaagg tgtggccaat tttattggtg aattaataac gggcactacc
     2761 aattacggcg aaaccgatac atatggttat gcgcatgaag ctgagttatg gaacgaattt
     2821 aaatctacgt tatgcgcatc gaataccgat aattggttat acaatggcga caattcaaac
     2881 aatcgtcctg cagatttagg gtattttatt ggttaccgca ttgcaaaaac ctattatcaa
     2941 aaatctgcca acaaacgaca agctgttatc gatttaatcg aaattgacga cccactttct
     3001 ttattggcaa aaagtggtta tgatggtaag taattgtact gtataattat tcctcagcta
     3061 atttttttac tacctccagt gcttttggaa aggtgctgtt gaaataggtt tcaaaccatt
     3121 cttcagcttc catattaagt tcaactttga gtgtagttac gccatctttt tcgtccatga
     3181 aataactttc agtagaagcg ccccattgtt tgggaatcct ttctccgttt ttaatttctc
     3241 ctaagtgttt aaatatcatt gtatgaaagt catctttttt aacaatcata ctaaacatgc
     3301 catcaccatt aggcccaaga aataaaatac tgcctccctc ctgccagtca ctaacagcat
     3361 gagaaccttc atgaaaaact gttgtccatt tgcggtatga agcatcatcc caaagcacat
     3421 cccaaacttt ctctttggat gcgttaattg tagtattaaa aacgagtatt gccatgattt
     3481 tatctatttt aatttataca gctaaaatac tacaggaact aacgaattcc aatttatttg
     3541 cgacaataaa acagggtgtt tgcgacaaag ttttgttaaa gttggagaaa tatatcctgc
     3601 ttccttaatc ggtctggaga acggagtggc gtctggagtc ggagactcca cgacaacgga
     3661 tgcatgaaat aattatttca tgactttaaa aatagttaaa ccatttccta tttatttaca
     3721 gctgttggat acgccgtcca tgtataactt ttttgcacaa ttttccagga accttcatac
     3781 tttaagagta aaaaatagtc ggtaaaaatt cgccaacccg gaataataat ttcagctttt
     3841 gccattgcgg cattattttc atagtcaatg gctattatgc gtcctattct atccgatttt
     3901 tcgccttctt taacgccttg aatatattct tcgcccgaac gaatgcgcag aatgtcttct
     3961 tgtgttacag tatacaaatt aaaatcgggg tgaaatgctc ttcgtaaccg ctcaggctcc
     4021 ccattggcag tcccttcaat ataatccatc aatgtagctg taatttgagc gatgtcagac
     4081 tgagtagaat ctgattgggc atacccttta agcgtaatca acaatagcag aaatgccaag
     4141 aaatgttttt gtattttcat aaagttgtaa ttatgaacta aatataagtg taatttacta
     4201 attgctttcc aatatagctt tcattttttc attcatcgcg ataaactttc ggtaagtatc
     4261 caggtatatt tttttaccat aatttttccc gaatatgcca tgccaatatt caccctgaat
     4321 aaaaagtatt tgggtgtcat taattcgctc aataataaaa taatgtttgg cgctaaaaag
     4381 aaaacctaac gaaccacccc atgctaattg cttatcaggt tcccactcca aaataaaagc
     4441 cgaaaattga tcggtagttg aatcagtgtc aattgttgtc accttaattt tttccttcag
     4501 tgaaacttca ccatcaattt ttacaatata tggatgccag ttggcataat cattaaaatt
     4561 tgtcagcaat tgccaggttt cggttgtggt ggtattaatt atgatttctg ttcggatttc
     4621 gttaggtgta cataattgct gcccccaaat atttattggt aaaaataaaa agcaaaataa
     4681 agtcaaattt tggctaatgg ttaaattgcg cataagttca ttgcgcaatt ataaagcttt
     4741 cttaagttat aaattcgttt gtaataattt cttgataaaa taaatactgg aagtattgct
     4801 attaatcatt cagccaactt taccgtaaaa ttgaagcacc atcaataaaa aaaggtccag
     4861 cgtctgctga accttgtacc cggggcggga cttgaacccg cacgaacatt gctgcccaca
     4921 ggattttaag tcctgcgtgt ctaccagttc caccacccgg gcatcactac ttgtgtagta
     4981 ttttatagtt tgttttcatt tcttccgttg aagaaataag ctattggtaa tcttgttggt
     5041 atccactatt tttttcaata ttggttactc gtttgtaact ccctcgtgag ggatttgtgg
     5101 ctgcaaattt acatacaagt tttatatgta gcatcataaa ataaaaaaag gatgaggccg
     5161 aggc
//
LOCUS       DAORKW010000232         5131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_701_length_5131_cov_4.836288, whole genome shotgun sequence.
ACCESSION   DAORKW010000232 DAORKW010000000
VERSION     DAORKW010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_701_length_5131_cov_4.836288"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLB46_09810"
     CDS             complement(<1..408)
                     /locus_tag="PLB46_09810"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HRF76855.1"
                     /translation="MYKFLVVMILVLGAGVVSAQHTFSICAVDPLTGEVGSAGASCIE
                     NSRIISDIHPGVGVIHTQAYYIAGNQNYAESLMDEGVAPSAIIDSMISNDIFDDPSFR
                     QYGVVDLVDGGRSAGYTGENTMDWKGHLTGPTYA"
     gene            502..1608
                     /locus_tag="PLB46_09815"
     CDS             502..1608
                     /locus_tag="PLB46_09815"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF76856.1"
                     /translation="MAFGINSRRIKKALRRLKSLPGGLNFFKYTANKVHAWYLKKTKS
                     TRVAHPSSIMLEVTNHCNLKCITCPREYQYGEEMEKGFMDIKQLKKIIDEVYPYVDSI
                     GLTGLGETLLYKQLPEALKYIRSKNKGIITTISINAHLPNSVELVADIADHLDTLQIS
                     MDGVGEVYENVRLRGEYPLFYDNVKRIVELCKDKRADVMFNFVAIKENYFTMAEVVEI
                     ASELGVDYINVTPFNVAAVTAHDISYYDFFHSDAFKNELKRAKDKADELKNVELTIWD
                     IKSLNEFKKCHLPWGHFYISWDGYATPCCAKPFPKELNFGNVFDDGLMYCLNSPGYLQ
                     FRQLWHENKTPSFCEKCHMVDLKPIHLPFELPVN"
     gene            complement(1605..1826)
                     /locus_tag="PLB46_09820"
     CDS             complement(1605..1826)
                     /locus_tag="PLB46_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76857.1"
                     /translation="MTAATFDLVKDIIVLLFFYLGAAWMWGVIKLGNENQTKLETIFA
                     RSRKRFKWLFLVGAVALTGLFLYQYLIAA"
     gene            1968..2729
                     /locus_tag="PLB46_09825"
     CDS             1968..2729
                     /locus_tag="PLB46_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76858.1"
                     /translation="MYTNIGWTGYTYSDKFDETGEIQPLNFDVTDNTFQLFAQYGITD
                     KLTVQTSIPYKFIKRSDYYGITTNPVPPPDYYTGDTLHYFGNLELGGIYKIFDGKPMV
                     SVSLMASLNSTDRNYISGLQTGFNSNAIKPGAGVMWSYNKAWLQYYLGAEIRDNNYPM
                     ALISNLEYGFKFGNAFYLAAQAYMRFPEPGENDCDCTTTLTAMYNSNQTYVGLGLKGG
                     YSYKAFGINAGLNTAFFAKNAPAQIAPSISLQYKY"
     gene            complement(2730..3326)
                     /gene="coaE"
                     /locus_tag="PLB46_09830"
     CDS             complement(2730..3326)
                     /gene="coaE"
                     /locus_tag="PLB46_09830"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRF76859.1"
                     /translation="MIKIGITGGIGSGKTTVCKIFEVLGIPIYYADDRAKELLESDKN
                     VIKKVKNIFGDDVYNEQGKLDRQRVATIVFNFPEILAEYNAVIHPAVFQDAEKWMLRH
                     SHHEYVIKEAALLFETGSYKMLDKIICVTAPVEKRIERVMLRDKVTEEQVRARMQNQW
                     SDAQKTAMSDYIIYNDGSTPLIRQVLSIHEKILIYAKM"
     gene            complement(3323..4243)
                     /locus_tag="PLB46_09835"
     CDS             complement(3323..4243)
                     /locus_tag="PLB46_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HRF76860.1"
                     /translation="MTFAQTSANATNNKKGRIVIRFLFCLFIAFILWLFNTLANQHQD
                     TIFVNLRYELAENKISQNKLPAQAELQVVSTGWQLLKEQFRVRSLSIDLAAYSENAQL
                     LTNLHKNLFSADLPEDIVVNHIFPDTLNMQIENKLSATIPVKMRFKGIVENHWEIDSL
                     YFYPDSITVTGPESIVQQFTYWPTESITIFDLDSNLRGVAILSDVSQVNLTLSAAVVA
                     YGIRVFKYTNQTYTFLLPNPMYKDRTVTVKLLCQVPSMKTAETSEQDFKINLLPLGPK
                     GMYAVKCEQFPDWVQNITVQPTFISVTSKE"
     gene            complement(4262..4603)
                     /gene="yajC"
                     /locus_tag="PLB46_09840"
     CDS             complement(4262..4603)
                     /gene="yajC"
                     /locus_tag="PLB46_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRF76861.1"
                     /translation="MLEQILLFAPAPEGQEAAGNSSIMSIAFFVLFFLILYFFMIRPQ
                     SKKAKEQKSFLNEIKAGDRVVTIGGVHGKILKVDDDTYLLEVDANTKVRIEKSVISLD
                     FTKAMLARKQA"
     gene            complement(4625..5095)
                     /locus_tag="PLB46_09845"
     CDS             complement(4625..5095)
                     /locus_tag="PLB46_09845"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRF76862.1"
                     /translation="MKKVHFTSILFAVAISATALVSCKEQPANTIETEATQEEDKLST
                     KIVENPTQITFEKDLHDFGEIIQGEKVSTEFKFTNTGKNDLLISDAYGSCGCTVPEWP
                     KEPIAPGQSGVIKVQFNSANRSGLFNKTVTVVANTQPSSNSKLIIKGTIIVPKD"
BASE COUNT         1603 a          923 c          938 g         1667 t
ORIGIN      
        1 ggcataagta ggtccggtta aatggccttt ccagtccatg gtattttcgc ctgtataccc
       61 tgcgcttcga ccaccatcaa ccaaatccac gacaccatat tgacgaaaag atgggtcatc
      121 aaaaatatcg ttgctaatca tagaatcaat tatggcagat ggtgcaacac cttcatccat
      181 aagactttct gcataatttt gattacctgc tatgtaataa gcctgggtat gtataacacc
      241 aacgccggga tgtatatcag aaataattct ggaattttcg atacatgaag cgcctgcact
      301 gcctacttct ccggttaggg ggtcaacagc acatatagaa aacgtatgct gggctgaaac
      361 aactccggcg ccaagcacca atatcatcac gacaagaaac ttatacataa aaattacagt
      421 tatggtgtaa agttaatcat ttagccccga tgttataaat acacctgata agctccatac
      481 gaatttgtaa ttttgcaccc aatggccttt ggtatcaaca gtagaagaat aaaaaaagca
      541 ttacgcaggt taaaaagttt acctggcgga cttaactttt ttaaatacac cgccaataag
      601 gtgcatgcct ggtatttgaa aaaaacaaaa agcacccgcg ttgcacatcc atcttcaatc
      661 atgttggaag ttaccaatca ctgtaattta aagtgtatca cctgcccaag ggaataccaa
      721 tatggtgaag aaatggaaaa gggctttatg gatattaaac aactcaaaaa aattattgac
      781 gaagtttatc catatgttga ttcaattggg ttaaccggtt taggtgaaac tttattgtac
      841 aaacagttac ccgaagcgct gaaatatatc cgcagtaaaa acaaaggcat tattactact
      901 atttccatta atgcgcattt gcctaatagt gttgaattag ttgctgatat agccgatcat
      961 ctggatactt tacaaatttc gatggatggg gtaggtgagg tgtatgaaaa tgtgcgttta
     1021 cgtggtgaat atccattgtt ttacgataat gtaaaacgta tcgttgaatt gtgtaaagat
     1081 aaacgtgcag atgtaatgtt caactttgtg gctattaaag aaaattattt cactatggct
     1141 gaagtagtgg aaatagctag cgaattaggt gttgattata tcaatgttac accgtttaat
     1201 gttgcagctg ttaccgcaca tgatattagt tattacgatt ttttccatag cgatgcattt
     1261 aaaaacgaat taaaacgcgc aaaggataaa gccgacgaat taaaaaatgt tgaacttacc
     1321 atttgggata ttaaatcctt aaatgaattt aaaaaatgtc atttaccttg gggacatttt
     1381 tatatcagtt gggatggtta tgccacacca tgttgtgcga aaccatttcc aaaagaactc
     1441 aatttcggta atgtatttga cgatggattg atgtattgtt taaacagtcc cggatactta
     1501 caattccgtc aactatggca cgaaaataaa actccttcgt tttgtgaaaa atgccatatg
     1561 gtggatttga aacccattca tctgccattt gaattgccgg taaattaagc tgcaatcaga
     1621 tattgataca ggaaaagtcc tgttaaagct actgctccaa ccagaaataa ccatttgaac
     1681 cttttgcgac tgcgtgcaaa tatggtttcc agtttggtct gattttcatt ccctaacttg
     1741 attacacccc acatccatgc agcacccaga taaaaaaaca ggagcacaat aatatctttt
     1801 acaaggtcga aggttgcagc agtcataagt tgaagttagt tggaaatatg tatttttgaa
     1861 tattcaaacg cgaaaatatg ctaatacaac ggatttctct gatatgtagc cttttatttc
     1921 tgttcgttag ccttaatgct caggtgtgga cactgcctaa aaatagcgtg tacaccaata
     1981 ttggttggac aggttatacc tattctgaca aatttgatga gaccggagaa atacaaccct
     2041 tgaattttga tgtaaccgat aatacgtttc agttatttgc acaatatggt attacggata
     2101 agttaactgt tcagacttcc ataccgtata agtttataaa acgctcagat tattatggaa
     2161 ttaccactaa ccctgttccg cctcctgatt attataccgg tgatactttg cactattttg
     2221 gcaatttaga attaggtggt atttataaaa tatttgatgg caaacccatg gtaagtgtgt
     2281 cgttaatggc aagtttaaat tccaccgacc gcaattatat ttcaggatta caaaccggat
     2341 ttaacagcaa tgcaataaag ccgggtgcag gtgtcatgtg gtcttacaac aaggcgtggt
     2401 tacaatatta ccttggtgca gaaattcgtg ataataatta tccaatggca ttaatttcta
     2461 atttggaata cggatttaaa ttcggcaacg cattttattt agcagcacag gcatatatgc
     2521 gatttcctga acccggtgaa aatgattgtg attgcacaac aacgcttacg gccatgtata
     2581 acagtaatca aacctatgtt gggttgggat taaaaggagg gtacagttat aaagcttttg
     2641 gaattaatgc gggtttaaac acagcatttt ttgctaaaaa tgcacctgca caaatagcac
     2701 ctagtattag tttgcaatac aaatattaat tacattttgg cgtaaatgag tattttctca
     2761 tgaatactca atacttgcct gataagggga gtgctaccat cattataaat aatataatcg
     2821 ctcattgcag ttttttgtgc gtcgctccat tgattttgca tacgtgcacg gacttgttcc
     2881 tcggtaactt tatcgcgtag cataacgcgc tctatacgtt tttcgacagg agctgttaca
     2941 caaataattt tatctaacat tttataacta cctgtttcaa acaggagagc agcttcttta
     3001 attacatact catgatggga atgacgcaac atccattttt ctgcatcctg aaatacagca
     3061 ggatgaatta cagcattata ctcggctaat atttccggga aattaaatac gattgttgca
     3121 acgcgctgcc ggtccaattt accctgctca ttatatacat catcaccaaa aatgtttttt
     3181 acttttttaa tgacattttt atcagactct aataattctt ttgcacgatc atcagcataa
     3241 taaattggaa tacccaatac ttcgaatatt ttacaaacag tagttttgcc actgccaatg
     3301 ccaccggtaa ttccaatttt tatcattctt tcgaagttac tgaaataaac gttggttgaa
     3361 cagtaatatt ttgaacccag tcgggaaatt gttcacattt aacagcatac atccctttag
     3421 gcccgagcgg caggaggttt attttgaaat cttgttcgct ggtctcagct gttttcattg
     3481 aaggaacctg gcataataac ttaaccgtta cagttcggtc tttatacatc gggttaggca
     3541 gtaggaaagt ataggtttga ttggtatatt taaatacccg aattccgtat gcaactactg
     3601 ctgctgataa ggttaaattt acttgcgata catcactgag tattgcaact ccgcgtaaat
     3661 tagaatctaa atcaaaaatg gttatcgatt cagttggcca ataagtaaat tgttgtacaa
     3721 tactttctgg tccggttacg gtaatgctat ccggataaaa atataatgaa tctatttccc
     3781 aatgattttc tacaattcct ttaaaccgca tttttaccgg aatggtagca ctgagtttat
     3841 tttcaatttg catatttaaa gtatccggaa aaatatgatt aacaacaata tcttcaggta
     3901 aatcggcact aaataaattt ttatgtaaat tggtaagtaa ttgtgcattt tcagaatatg
     3961 cggctaggtc aatactcagc gaccttacac gaaattgttc ttttaataat tgccagccgg
     4021 tagatacaac ttgtaattcg gcttgagcag gtaatttgtt ctgactgatt ttattttcag
     4081 ctaattcgta acggagattt acaaatatgg tatcctgatg ttggttggcg agggtattaa
     4141 ataaccacag gataaaagca ataaaaaggc agaataggaa tcgtattaca attctgcctt
     4201 ttttattgtt tgttgcattc gcagatgttt gggcaaatgt caattattag tagctaaatt
     4261 gttaggcttg tttacgcgcc aacatggctt tggtaaaatc aagcgaaatt actgactttt
     4321 caatgcgtac tttggtattg gcatcaactt ccaataaata agtatcatcg tctactttta
     4381 atattttgcc gtgaacacca ccaatggtta caactctgtc gccggctttg atttcattta
     4441 aaaatgattt ttgttctttc gcttttttag attgcggacg gatcataaaa aagtaaagta
     4501 tcagaaaaaa caatacaaaa aaagcaatgc tcataatgct tgagtttcct gcagcttcct
     4561 gaccttccgg tgccggtgca aataataaga tttgttctaa catgtatttc gatttattgg
     4621 ttaattagtc tttcggaaca ataatagttc ctttaataat gagttttgaa ttgctgcttg
     4681 gctgagtgtt ggcaacaaca gttactgttt tattaaataa tccgctgcgg ttggcgctgt
     4741 taaactgcac tttaattaca ccactttgtc ccggagcaat tggttctttt ggccattccg
     4801 gaacagtgca tccacatgaa ccataagcat cagatatcag tagatcattt ttaccggtat
     4861 tggtgaattt aaattcagtg cttacttttt cgccctgaat tatttcaccg aaatcgtgta
     4921 aatctttttc gaatgtaatt tgcgtaggat tttctacaat tttggtcgac agtttatctt
     4981 cttcctgtgt tgcttctgtt tcaattgtat tagcaggttg ttctttacat gaaaccaatg
     5041 cagtcgcgct aatggcaaca gcaaataaaa tggaagtaaa atgtactttt ttcatattac
     5101 aaatttaatg gtaattattc gattaaacca c
//
LOCUS       DAORKW010000233         5102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_710_length_5102_cov_5.272637, whole genome shotgun sequence.
ACCESSION   DAORKW010000233 DAORKW010000000
VERSION     DAORKW010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_710_length_5102_cov_5.272637"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1179)
                     /locus_tag="PLB46_09850"
     CDS             complement(49..1179)
                     /locus_tag="PLB46_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76863.1"
                     /translation="MNKIIKMILVLHLAFGAKVMHAQCLITAVKPGEMFTLDQLWNVV
                     VSGNLPPGQYKVGLTLSSAKNLVYKAETRAFNYNNNSITISFANKRMFEPLVVQYMDP
                     NFNTALMQAAGIVPDGRYTVAFTLIDAGTNTEELCKTSYRLIVEKNIAPLQLLFVHKN
                     DTIKESCPTFGWIPPFPLPTGDYNYELLLTECYDNQTAFDATTSNLPVYKMTVGKNIQ
                     KNVCSDLAKLEPGKSYAWKINLYQNGKFDNTSESWKFFYEPEDSANLIEPEKYFHMDD
                     KVYGSYVVIDSNLLPIYFKEDYNVIDSIINVVLYDSAMNVYAEGEHIMLAYNNGSNHS
                     YLNFCNYDFELAKGMYLLEITCITQKKYFIRFKNKSELTDCY"
     gene            complement(1185..3755)
                     /locus_tag="PLB46_09855"
     CDS             complement(1185..3755)
                     /locus_tag="PLB46_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76864.1"
                     /translation="MKKHFSRYRKSIARYFLMGIMTLFINMSYSQLYIKYDEHNGKVK
                     SGADLWQFNCINTSSDSLLCDITILLTDASGKPLYFVNAHKVKFGAKQALSGKQLYEY
                     ATEQTVTENELSAILKQVKSMCVSANHVATQFEIVTNCRDLQNGMSDTSKVVEAFTKI
                     GESINAQVAIAQYVNLKPENESGMLLKPQFSGYANFTTFGVPFQLQFYNAYAAEIFGF
                     KPAMALTFDSAGYKQQLTEKMEDKLAGEMHSKGLNYEMIYAKLTAYEGLQSYFNNFDS
                     LLVDSLTAIANVKKSEYIAKAQDSLYNLSAQLKNNTLATYDSVNNCFNDDNYLLDSVM
                     SIENSIGQLAQEKNGVDSLIGNYQNLLDLNAEKENLYQQLYEDSTVASYVKQIEQINA
                     IKSGEISQFAALIDTGKFAAATKVISNVNDFGAGKIVYNVDAFLLENVSISGAVLDYQ
                     INEYTSVFGLGGFMKDEFDYYTAVNSLTNTNRIIAGGIKREIGQFGKYTMAMLDGRNH
                     DTTIAYNQHWNGNNLIFNKYEALGLEDGLFDLEMSLGLSITSNDALENDVVQDANWFP
                     QMFTINGAYIDNTFSGFATRISTTFHMLKWQSSCFVKHEFINKSYFTAGNPFLLTGLQ
                     IFETGWETKFEKIKTDIALGAIYNLFFKEVDNTKRTGFMQYKGEISSEITDDLHLSLN
                     YYPNVIFLNGQKTVVSNLVGTVSYTKAIQSLPVTSILTYAQTNSSQLDAENTAIHFQQ
                     HMAIGMVNMSINKHLSTGVNMQFAALAYDSNYTTLSVGQNVVVSLADSWEIMGDYSLP
                     IKSNINTEMAYRLNAGYLFKKIKLGAEVMKMEYNMLNAGLENSTMVVWGNVYCTFNIN
                     "
     gene            4605..>5102
                     /locus_tag="PLB46_09860"
     CDS             4605..>5102
                     /locus_tag="PLB46_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRF76865.1"
                     /translation="MSFDKLLLIEPIMRALKQEGYERPTPIQEQAIPAALEHKDLLGC
                     AQTGTGKTAAFAIPVLQILAAKKHDNHRVIRSLILTPTRELAIQIGESFTAYGKYTGL
                     THTVIFGGVSQHAQTQQLRRGVDILIATPGRLLDLMNQGFVRIDKVEIFVLDEADRML
                     DMGFVH"
BASE COUNT         1599 a          914 c          814 g         1775 t
ORIGIN      
        1 cttttacgta tggaagttaa tatatgcata gattattttt ttagaatttt aataacaatc
       61 ggttaattca cttttgtttt taaatcgaat aaaatatttt ttttgtgtaa tgcaggtaat
      121 ttcaagtaaa tacatgcctt ttgctaattc aaaatcataa ttgcaaaaat tgagatagga
      181 atgatttgag ccattattat atgctaacat tatatgttca ccttctgcat acacattcat
      241 ggccgaatcg tataatacaa cattgattat actgtcaatt acattataat cttccttaaa
      301 ataaataggt aataaattgc tgtcaattac cacataagaa ccatatacct tgtcatccat
      361 gtgaaaatat ttttccggtt ctatcaggtt tgcactatcc tccggctcat aaaaaaactt
      421 ccaggactca ctggtattgt caaatttgcc attttggtat aaattgattt tccaggcgta
      481 agatttaccc ggttctaatt ttgctaaatc gctacaaaca tttttttgga tgtttttccc
      541 aaccgtcatt ttgtaaacag gcaaattgct tgtggttgca tcaaatgctg tttggttatc
      601 gtaacattct gtcagcaaca attcataatt ataatcgccc gttggcaacg ggaatggagg
      661 tatccagcca aatgttgggc aactttcttt tattgtatca tttttatgaa caaacaagag
      721 ttgaagtgga gcaatatttt tttcaacgat taatctataa cttgttttac ataattcttc
      781 tgtatttgtg cctgcatcaa ttaaagtaaa agcaacggtg taccggccat caggtacaat
      841 accagcagcc tgcataagcg ctgtattaaa attgggatcc atatattgca ccaccaatgg
      901 ctcaaacatt cttttatttg caaaacttat tgttatgcta ttgttattgt aattaaaggc
      961 tcttgtttct gctttgtaaa ccagattttt tgcagaacta agcgttaatc ctactttata
     1021 ttgccccggt ggtagattac ctgaaacaac cacattccag agttgatcaa gagtaaacat
     1081 ctcaccaggt tttactgctg taattaaaca ttgagcatgc atcacctttg cgccaaaggc
     1141 aagatgcagg actaaaatca ttttgataat tttattcata tttatcaatt tatattaaat
     1201 gtgcaataaa catttcccca taccaccatg gttgaatttt ccaacccggc attcaacata
     1261 ttatattcca tcttcataac ctcagctcct agttttattt ttttaaataa gtaacctgca
     1321 tttaaacgat atgccatttc tgtattaata tttgatttaa tcggcaaact ataatctccc
     1381 ataatctccc aactgtccgc taggcttact actacatttt gtccaaccga aagtgtggtg
     1441 taattgctat cgtatgctaa tgcagcaaat tgcatattaa ctccggtgga aaggtgttta
     1501 ttgatggaca tgttaaccat acctatagcc atatgttgtt gaaaatggat ggcagtattt
     1561 tctgcatcta attgtgacga attagtttgt gcatacgtta aaatactcgt aaccggcaat
     1621 gattggatag cttttgtata acttactgtc ccaaccaaat ttgatacaac tgttttttgt
     1681 ccattcaaaa aaataacatt tggataataa ttaagcgaaa ggtgcaggtc atctgtaatt
     1741 tccgatgaaa tttcaccttt atactgcata aatcctgttc ttttagtatt atctacctct
     1801 ttaaaaaata aattataaat tgctcctaaa gcaatatctg ttttaatttt ttcgaatttt
     1861 gtttcccatc cggtctcaaa tatttgcagc ccggtcagta gaaaaggatt accagcggta
     1921 aaataggatt tattaataaa ttcatgttta acaaagcagc tggattgcca ttttaacata
     1981 tgaaatgttg tgctgatacg agttgcaaag ccgctaaagg tattatctat ataagcgcca
     2041 ttaatagtaa acatttgcgg gaaccaattg gcatcttgca caacatcatt ttccaatgca
     2101 tcgttgcttg ttattgacaa tcccaacgac atttccaaat caaacaaacc gtcctctaaa
     2161 cctaatgctt cgtatttatt aaatattaag ttattgccgt tccaatgttg gttgtaagca
     2221 attgttgtat catgattacg gccatcaagc attgccatag tatatttacc aaattgccct
     2281 atttcgcgct taataccacc agcaattatt ctattggtgt tcgttaaact attcacagca
     2341 gtataataat caaactcatc tttcataaat ccacctagtc cgaaaacgga ggtatattca
     2401 ttaatttgat aatctaaaac agccccgctg atagacacat tttcgagcaa aaatgcatca
     2461 acattataca cgatttttcc ggcaccaaaa tcattaacat tgctgattac ttttgtcgca
     2521 gcggcaaatt ttcctgtatc aatcagcgca gcgaattgac taatttcgcc tgatttaatg
     2581 gcattaattt gctctatttg ttttacatat gaagctacag tcgaatcttc atatagttgt
     2641 tgatataaat tctctttttc tgcattcagg tccaataaat tttgataatt cccaattagt
     2701 gaatccacgc catttttttc ctgagccaat tgcccaattg aattttcaat actcattacc
     2761 gaatcaagca ggtaattatc atcattaaaa caattattaa ctgagtcgta ggtggctaag
     2821 gtgttgtttt tgagttgtgc tgacaagtta tacaatgaat cctgtgcttt ggctatgtat
     2881 tcagacttct tcacattggc tattgctgtt agcgaatcta caagcagtga atcgaaattg
     2941 ttaaaatatg attgcagtcc ttcgtacgca gttagtttgg cgtaaatcat ttcataattt
     3001 aaccccttgc tatgcatctc gccggctaat ttatcttcca ttttttcggt tagttgttgt
     3061 ttataaccgg cggagtcaaa ggtcaatgcc atagcaggtt taaacccgaa tatttctgcg
     3121 gcataggcat tataaaattg cagttgaaat ggcacgccga aagtagtaaa attggcatat
     3181 cccgaaaatt gtggcttaag cagcattcca ctttcatttt ccggttttaa attcacgtat
     3241 tgtgcaatag caacttgtgc attaatggat tctccaattt ttgtaaatgc ttctaccact
     3301 ttagaagtat cactcatccc attctgtaaa tcacgacaat tagtaactat ttcaaattgt
     3361 gttgcaacgt gattagcact aacgcacatt gattttactt gttttaggat agcggataat
     3421 tcattttcag ttactgtttg ttctgtagcg tattcgtata attgtttacc agataaagct
     3481 tgtttagcgc cgaatttaac cttgtgtgca ttcacaaaat aaagcggttt accggatgca
     3541 tcggtaagta aaatagttat gtcacataat aaggaatctg atgaagtgtt tatacaatta
     3601 aattgccata aatcagcacc ggattttact ttaccattgt gttcatcata tttaatatat
     3661 aattgactat aactcatatt aataaataag gtcataattc ccattaaaaa ataacgggca
     3721 atacttttcc tgtatcttga aaaatgtttt ttcaaaatga ataatagttt agctatccga
     3781 tttgtaaata aatcagcaca aatcaatgag atgcttagca ccacaaggtt tgccgcaaat
     3841 cgtgacagta ataaaatagg ttagccaact gggatgatag tgtggttgac taaaatagaa
     3901 ttggattgtt ttttacggag ggttctcaat tctccgtgtt gtacgtaatt taagggtttt
     3961 tatttaaggg ttaaaaaaat tgttttcaaa taatgtaaat cgaaactatt agtctatttt
     4021 catatttcca aatttatttt aaagtttttt gtaaaaaaaa tccaagttgt tgaattacaa
     4081 tacatttgtt aaataattgc caaaattttg attactaatg cattaaaaaa tgtaacaaat
     4141 tgcttttatt gtgacattaa atacatgtcc atttggtttg tgcaagaact cattacgatt
     4201 accttacttt ttcttcacgt tttgtagata tgtattgatt ttgaatagtc tatgaaaaag
     4261 gttgttgtta ataaatgtcc taatcactta ctttttgctt ctcaatcgat ttaagatgga
     4321 tataatacac atccgatgag cacctatgcg tattattcat cccaaaccta ttcacatggc
     4381 gtaaaacctg tggttaggta tattcatgat aaatcattat ctttgcaccg ctttaggcct
     4441 aataccgttt ctgaggtgtg ttcattacga aatacaaagt attaaaaacc aaaagcattt
     4501 attttcatcg taaggcgtta aagacttaga gaagaacact gcattaaagc attcgcacat
     4561 atgcatctgt aacatttccc tccttataca aattttattg atacatgtca tttgacaaat
     4621 tattgctcat tgagccaatc atgagggcat taaaacagga aggttatgag cgccctactc
     4681 ctattcagga acaagccata cctgccgcat tagagcacaa agatttatta ggttgtgcgc
     4741 aaacaggaac aggaaaaact gcagcatttg ccattccggt attacaaatt ttagctgcaa
     4801 aaaaacatga taatcaccgc gtaattcgct ccttaatttt aacaccaaca cgtgaattgg
     4861 ccattcaaat tggtgaaagt tttaccgctt atggtaaata cacaggttta acacatactg
     4921 taatttttgg tggcgtatcg caacatgcac aaacacaaca attacgcaga ggggttgata
     4981 ttttaatcgc tacaccgggt cgtttactcg atttgatgaa ccagggattt gtccgcatcg
     5041 acaaagtgga aatttttgtt ttggatgaag cagatagaat gctcgatatg ggttttgttc
     5101 ac
//
LOCUS       DAORKW010000234         5095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_712_length_5095_cov_5.525198, whole genome shotgun sequence.
ACCESSION   DAORKW010000234 DAORKW010000000
VERSION     DAORKW010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_712_length_5095_cov_5.525198"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PLB46_09865"
     CDS             <1..924
                     /locus_tag="PLB46_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laminin B domain-containing protein"
                     /protein_id="HRF76866.1"
                     /translation="TWYFNSPAIFNIDLSGFYGGYLTFDQKQNSSTDQTNVEDIMIEK
                     SDGTKIVYNTPSNPDILSWTTYNVPLIEAGWKYTNLAGGAVSYTDFMDYLTNIHVIKI
                     RGDYSSDKFETTWMDNVKIITSLLPVEIGDFSGVLQFNATAVINWETLTEQNVSHFGI
                     QKSTDGGRTFETIGKVNAIGNSTEANLYTFVDEACYGETYYRLLVVDADNSSAFSKVI
                     VVSGTEISASDIQLFPNPASSQIVINNTNTAFAFESIIIVDTYGRTVKSIDATGADNY
                     TIDLSSLAEGFYFASLKNKSTAYSVPFQVIH"
     gene            1040..2491
                     /locus_tag="PLB46_09870"
     CDS             1040..2491
                     /locus_tag="PLB46_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF76867.1"
                     /translation="MKKFSLLTLSIMLAAFFTSKVNAQITLYEYTTNVNGDANYVDPN
                     LSADKLRRVKGASGTGAGIICPIGFTSKGFSTSEIWADNLPSVVATLIPGAGVDAVVG
                     KVGIEMRITETGPTYVRLAYSTDLGVTWVDAGVDATPPISDCGDITLYYEWDMPDFTA
                     TDKFYIRVYAFGAVSETGRFNISQLKVYGALDMIDADGDGYGEAIDCDETNPDIHPGA
                     EEICNAIDEDCNGESDEVAVSITPTGEIYLCNNEETVELFATPGYDSYQWYKNGSPIP
                     VTTESIIIENPGYYQVEATQGLCTAISAVQAIASSESPFANIYYPDGLDLCVDDSLLI
                     KASYDELYNWQWYRDGSPIMDETNYKMLALVPGTYYCEVTTVLGCTRNTEEIVITTCR
                     VGNVNAINNMTAYPNPANTTISFNIQTGNTTADAVLSIVSLNGQEVINMPVEIVDGLF
                     NTSINIEALTNGIYTARLITGENQFTSRFTVVK"
     gene            complement(2555..3808)
                     /locus_tag="PLB46_09875"
     CDS             complement(2555..3808)
                     /locus_tag="PLB46_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76868.1"
                     /translation="MKNFIWGFGLLCCNFTYAQSRIVINNDASIVMSNGVNIVVDNAN
                     TNAIVTSGTGGNIISEAETNIVKWNIGTSTGNYIVPFTAFSGNKIPVSLNISTAGSGS
                     GSILFSTYNGISWNNDTYKPSGVTNMTNMAAVNNSSDVIDRFWMIEAQGYSTKPAGDL
                     QLTYIDAEHTAIGNTITETDLKAERYEGVSDNWETFPVAGIVDIANNNVSGIYFSNSD
                     FMRVWTLLDQTTHTLPLTLLNFDIICEEVSTILTWSTAQEQNTSRFIISSSLDGINFN
                     PIATIPAIGNSSTTQHYQFIADGSSGNYYQLTLENEDLSQTDLGIVYSTCENAPAGMA
                     FAFASGLKSITLQTTNLPEGDYAIQIYDLQGHLLFMENLFITNNFNSYLLTNEYLSAG
                     MYILKLFNRNISNNFSYTQQIQFLR"
     gene            complement(3829..>5095)
                     /locus_tag="PLB46_09880"
     CDS             complement(3829..>5095)
                     /locus_tag="PLB46_09880"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRF76869.1"
                     /translation="GAGHDNTTGSNNIIIGTDAGLSSTTANNNVFIGLESGYANTIGY
                     DNAYVGTQTGRSNTTGYYNSAFGSYVLYANTTGNNNSAFGLNAMRYNSTGSNNTAVGM
                     SAMSANTSGVNNTALGLEALYTNTSGNYNLAVGYQSLRSSTTASSNSAVGTFALYACT
                     TGANNVAVGYNAARETTTGINNTAVGVQALNSNTTGSYNTVMGRDALAANLTGANNTA
                     VGYGSGGFNDANTNCTFLGYDADQAVTTNFDNSTAVGNGSRITASNQVRIGNASVTSI
                     GGYAAWTNISDGRIKTDVKQNVPGLDFIMQLKPVTYHLNIAAINNQLMIDDTLINQHA
                     VAEKSAIQYTGFIAQEVEAAANAINYNFSGVDKPQDESGLYGLRYAEFVVPIVQALQE
                     QQQIIEKQQTQIDTLINELALIKASLPTK"
BASE COUNT         1551 a          926 c          911 g         1707 t
ORIGIN      
        1 acttggtatt tcaatagtcc tgctattttc aatatcgatt tatccggctt ttacggcgga
       61 tacctgacgt ttgatcaaaa acagaattca agtaccgatc aaaccaatgt tgaagatatc
      121 atgattgaaa aaagcgatgg gactaaaatc gtgtataaca ctccatcgaa tccagatatt
      181 ctaagttgga ctacctataa tgtgccttta attgaagcag gttggaaata tactaatctt
      241 gccggtgggg cagttagtta cacagatttt atggattacc tgactaatat tcatgtaatt
      301 aaaatacgtg gggactactc tagcgataaa tttgaaacca cctggatgga taatgtgaaa
      361 attattacct ccttattgcc tgttgaaatt ggtgatttta gtggtgtatt acaatttaat
      421 gctaccgctg ttatcaattg ggaaacactt actgaacaaa acgttagtca ttttggtatc
      481 cagaaaagta cagatggcgg aagaacattt gaaactatag gaaaagtaaa tgcaattggt
      541 aattcaacag aagccaacct ttatacattt gttgatgaag cctgttatgg tgaaacttat
      601 taccgattac ttgtagtgga tgccgacaat tcatctgcct tttctaaagt aattgttgtt
      661 tccggaactg aaataagtgc tagcgacata caattatttc caaatcctgc ttcatctcag
      721 atagtaatta acaatacgaa tacagctttt gccttcgaat caatcattat tgttgataca
      781 tatggcagaa cagtaaaaag tatcgatgct accggcgccg ataattatac tatcgactta
      841 agcagtctag ccgaaggatt ttactttgca tccttaaaaa acaaatcaac agcttactca
      901 gtgccttttc aggtaattca ttaagtatta aaaatattta ttgggtatcc tttacaacat
      961 tggcagggta acttgtctca tatattaaac acaatttatc aaacaactaa ttagtctaaa
     1021 tctaacactt attaaaatta tgaaaaagtt ctccttatta acgctgtcaa tcatgttggc
     1081 tgcgtttttt acctcaaagg taaatgcaca aattaccctt tacgaataca ccacaaacgt
     1141 aaatggtgat gctaattatg tagacccaaa cctttctgct gacaaactca gaagagtgaa
     1201 aggggcatcc ggcacaggcg ctggtattat atgccctatt ggttttacct ctaaaggctt
     1261 tagcacatcg gaaatttggg ccgataacct accaagcgtt gtagctacat taatacccgg
     1321 agccggtgtt gatgcagttg ttggcaaagt tggtattgaa atgcgaatta ctgaaacagg
     1381 tcctacttat gttcgtttag catatagcac cgacttaggt gttacttggg ttgatgcagg
     1441 tgtcgatgca acgcctccta tttctgattg tggtgatatt accctttatt atgaatggga
     1501 tatgcctgac tttacagcaa ccgataaatt ttatatccgt gtttatgcat tcggcgctgt
     1561 ttcagaaact ggtcgtttta atatcagcca acttaaagtt tatggcgcat tagatatgat
     1621 agatgctgat ggtgatggct acggtgaagc tatcgattgc gatgaaacaa atcctgatat
     1681 tcaccctggt gctgaagaaa tttgtaatgc aatagatgaa gattgtaacg gcgaatctga
     1741 tgaagtagct gtttcaatta caccaactgg tgaaatctac ttatgtaaca atgaagaaac
     1801 tgttgaactt tttgcaactc ctggttatga ttcttatcag tggtataaaa atggcagccc
     1861 aattccggtt actaccgaaa gtattataat tgaaaatcct ggttattatc aggttgaagc
     1921 aactcaaggt ctttgcacgg ctatttctgc tgttcaggct attgcttctt ctgaatcacc
     1981 ttttgctaat atctattatc cagatggttt agatctttgt gttgatgatt cattattaat
     2041 caaagctagt tacgatgaat tatataactg gcaatggtac cgcgatggtt ctccgattat
     2101 ggatgaaact aactacaaaa tgttagcact cgttcctgga acatactatt gcgaagtaac
     2161 cacagtttta ggttgcacca gaaatactga agaaattgta atcacaactt gccgtgttgg
     2221 taatgtaaat gcaatcaata atatgacggc atatccaaat ccggctaaca caacaatttc
     2281 atttaatatt caaacaggaa acactacagc tgatgcagtg ttatctattg taagtttaaa
     2341 cggacaggaa gtaattaata tgcctgttga aattgtagat ggtttattta atacttcaat
     2401 caatattgaa gcattaacaa acggtatcta tactgcgaga ttaattacag gtgaaaatca
     2461 attcacttct cgctttacag ttgttaagta atagtagctc aacaataagg atcaaagggt
     2521 cgatgtaaaa gtcggccctt tttttatttg gtatctatct taaaaattga atttgttgtg
     2581 tatatgagaa attatttgat atgttgcgat taaataattt caatatgtac attcccgccg
     2641 acaagtattc atttgttagg agataactat tgaaattatt ggtaataaac agattttcca
     2701 taaataataa atgaccttgt aaatcatata tttgaatggc ataatcacct tccggtaagt
     2761 tagttgtttg taacgtaata ctttttaatc ctgatgcaaa ggcaaatgcc atccccgcag
     2821 gtgcattttc acaagtgctg taaacaatgc ctaaatctgt ttgtgataaa tcttcatttt
     2881 ccagtgtgag ctgataataa ttgccacttg aaccatctgc aataaattga tagtgttgtg
     2941 ttgttgaact atttcctata gcgggtattg ttgcaatagg gttgaaattt atgccatcta
     3001 aactggagga aatgataaaa cgactggtat tttgttcttg ggctgttgac catgttaaaa
     3061 tggtagatac ttcttcacaa ataatatcaa aattcaataa tgttaaaggc aatgtatgtg
     3121 ttgtttggtc caataatgtc catacccgca taaaatcgct gttgctaaaa tatattcctg
     3181 aaacattatt atttgcaatg tcaactattc cagcaaccgg aaatgtttcc caattatcgg
     3241 aaactccttc ataacgttca gcttttaaat ctgtttcggt aatggtatta ccaattgcag
     3301 tatgctctgc gtcaatatag gtaagttgta aatcgcctgc gggttttgta ctatatcctt
     3361 gagcttcaat catccaaaat cggtcaatta catcactcga attattgacc gctgccatat
     3421 tggtcatatt ggtaactccg ctgggtttgt aggtgtcgtt attccatgaa ataccattat
     3481 atgtggaaaa taaaatggaa ccacttccac ttcctgcagt gctaatgttt aatgaaacag
     3541 gaattttatt tcccgaaaat gcagtaaacg gcacgatata atttccggta gaggtgccaa
     3601 tattccattt aacaatattt gtttctgctt cactgataat gttgcctcct gttccgcttg
     3661 ttacaatagc attggtgttt gcattatcca caacaatatt gacaccatta ctcattacaa
     3721 tactcgcatc attattaatg actattctgg attgcgcata ggtgaaattg caacacaata
     3781 aaccaaatcc ccaaataaaa tttttcataa ttggtaattt tattaaaatt atttagttgg
     3841 taaacttgct tttattaatg ccaattcatt aatcaaggta tcaatttgag tttgttgttt
     3901 ttcgataatt tgttgttgct cctgcaacgc ctgaactata ggaacaacaa actctgcata
     3961 tctcaatcca tataatccac tttcatcttg tggtttatct acaccactaa aattgtagtt
     4021 gattgcatta gcagctgctt caacttcctg agcaataaaa ccggtgtatt ggattgctga
     4081 tttttctgca acagcatgtt gattaattaa tgtatcatca atcattagtt gattattaat
     4141 tgctgctatg tttaaatgat acgttaccgg tttaagttgc ataataaaat ctaaacccgg
     4201 aacattttgt ttcacatctg tttttattct tccatcagaa atatttgtcc atgccgcata
     4261 accaccaata cttgttacgc tggcattgcc aattcttacc tgattacttg cagtaattcg
     4321 agaaccatta cccactgctg ttgagttatc gaaatttgtt gtaacagcct ggtcagcatc
     4381 gtatccaaga aatgtacaat ttgtgtttgc gtcattaaac ccaccggaac cgtaacctac
     4441 tgctgtattg tttgctcctg ttaaattggc agctaatgca tctctaccca taactgtatt
     4501 atatgacccg gttgtgttac tgttcaaagc ttgtacacca accgcagtgt tatttatacc
     4561 ggtggttgtt tcacgcgctg cattatatcc tacagcaaca ttgttggctc cggtagtgca
     4621 tgcatacaat gcaaatgtgc caactgcaga attagaactt gcagttgtac ttgaacgtaa
     4681 actttgatac ccaactgcca aattatagtt cccggaagta tttgtataaa gtgcctcaag
     4741 tcctaatgca gtattattaa ctccggatgt atttgcagac attgctgaca ttcctactgc
     4801 agtattgttt gaaccggtgg aattatatcg cattgcgttt aatccgaatg ctgaattgtt
     4861 attacctgtt gtgttggcat ataatacata tgaaccaaac gcactattgt aatatccggt
     4921 tgtatttgat ctgccggttt gtgttccaac atacgcgttg tcgtatccta ttgtgttggc
     4981 gtaacctgat tccaaaccaa taaaaacatt attatttgca gtggtacttg ataatccggc
     5041 atctgttcct ataataatat tattgctacc tgtagtattg tcatggccag ctcct
//
LOCUS       DAORKW010000235         5091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_713_length_5091_cov_4.358221, whole genome shotgun sequence.
ACCESSION   DAORKW010000235 DAORKW010000000
VERSION     DAORKW010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_713_length_5091_cov_4.358221"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLB46_09885"
     CDS             <1..204
                     /locus_tag="PLB46_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76870.1"
                     /translation="KYNYTTQEAAWQFDVTALLGVEGEISRVYNRKSSLVIIFKTHPK
                     LDDNFTCVSINTDTGNMEWVYKF"
     gene            complement(283..1836)
                     /locus_tag="PLB46_09890"
     CDS             complement(283..1836)
                     /locus_tag="PLB46_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HRF76871.1"
                     /translation="MERELIINSTPTGAEIALLEDKQLVEIHRDSQSSRFNVGDLYLG
                     RVKKITPGLNACFVDVGFERDAFLHYSDLNPNFRSIYKYTKAAQDGHPYSMDSFLKEP
                     EIIKTGKINNVVAKNQHILVQIIKEPIQTKGPRLSCEISLAGRYLVLTPFNEVVGVSR
                     KIASANERKRLQALIEQLRPKGFGVIIRTVAEGVATHLLHEDLNDLIAQWAEITKRLQ
                     QLQAPQIVYSETRKTNTLLRDVLNDSFNNVVVNEPSIANEVKEYIKKISPGSEKMVTL
                     HTTGKSVFDQFGVTKQIKSLFSRTVNMDSGAYLIVEHTEALHVIDVNSGNKTAVKGDQ
                     EQNAVAVNVEAAKEIARQLRLRDLGGIIIVDFIDMKHPDNKKAVYNALKEAMANDRAK
                     HTILPISKFGVAQITRQRVKPEVNITTTEVCPTCSGTGKIEASVLLIDDIERKIKYLV
                     KNQNQQYVKLIVHPFVESFIKKGRFFNSIQWKWYWEYKRKIHVSGSNEFQYMEYRFYD
                     KGDEEIHVE"
     gene            complement(2023..2811)
                     /locus_tag="PLB46_09895"
     CDS             complement(2023..2811)
                     /locus_tag="PLB46_09895"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF76872.1"
                     /translation="MKGIYIGVGLAAAAIAIIYFGFSNKPPVVTAFDDQRKKLIDSLQ
                     YPNGEQLMEMHHDMQKASASSKTALLQQLSDEWLQIGQPVIAADYLRQKAESEPTYEN
                     FMRAGSALSSLIDFVQEEDLRVSVVYGARFCFEEAGKLKPEDLDAKIGLATVLVSGTN
                     APMEGIMMLREIDAQHPDNIQVNLELGRFSVMSGQMDKALERFDRVLQKDSLNLQARY
                     MKAQTYLGLKDTSGAILELEKLKSMTTDSMIINQVRTEIHNLNH"
     gene            complement(2813..3112)
                     /locus_tag="PLB46_09900"
     CDS             complement(2813..3112)
                     /locus_tag="PLB46_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRF76873.1"
                     /translation="MRKADIVNRITEKTGLEKVDVMVVVEAFCKEIRESMAEGNNVYV
                     RGFGSFVVKTRKKKIGRIIKKNIAIEIPEQKIPAFKPAKVFINRVKEGKKSKASL"
     gene            3217..4275
                     /gene="mutY"
                     /locus_tag="PLB46_09905"
     CDS             3217..4275
                     /gene="mutY"
                     /locus_tag="PLB46_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRF76874.1"
                     /translation="MRKVFTQKLFRWYSLYKRNLPWIGSNDPYKVWLSEVILQQTRVE
                     QGLSYYQKFVKKYPDIQQLANAPHDEVMKMWEGLGYYSRARNMHSTAKQVVEELNGRF
                     PDTYEGLIQLKGIGQYTAHAILSYAYKKPFAVVDGNVLRVLARYFGIREAIDTPKGKS
                     LIQQHADSLLDKKEPDVFNQAMMDFGALICKPRQPDCENCPLQKSCAGYQQELTDILP
                     VKSKKIKRQTRYLHFFVFRAGEKILVEQRIANDIWKNLYQFPVIETSKNVSSKSLMES
                     EDYQAFRINNSLPVITSPIFKQQLTHQQLICRFYIFDLKSTDKIKRSGVKQVTFTQLK
                     QLAFPGVIRDFLKINDYF"
     gene            4306..4743
                     /locus_tag="PLB46_09910"
     CDS             4306..4743
                     /locus_tag="PLB46_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRF76875.1"
                     /translation="MSGVNKVILIGNLGKDPDLRNFEGGNVVANFTLATTEVYRDRNG
                     NKVEQTEWHNIAMWGKLAEIAGKILRKGAKVYIEGRIKNRSWEDREGNKRYITEIVAE
                     NFTLLGPRPGGNGTGAQGGTDYEMSHSNPDNEKDMIVDDSLPF"
     gene            4823..>5091
                     /locus_tag="PLB46_09915"
     CDS             4823..>5091
                     /locus_tag="PLB46_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76876.1"
                     /translation="MQKNLFRLLLVLLPAAFFISCETDTTPRPMGFPKVNYPIAKDTL
                     LYNDPNCPYTFLLPDYYEINNKVTFFNQAPENPCWMNLICKDLNAT"
BASE COUNT         1517 a          994 c          923 g         1657 t
ORIGIN      
        1 aaatacaact ataccacaca agaagcagca tggcaatttg atgtaactgc attgttaggt
       61 gttgaaggtg aaatttccag agtttacaat cgcaagtcga gtttggtaat catattcaaa
      121 acacatccga aactcgatga caatttcacc tgtgtttcaa taaatacaga caccggcaac
      181 atggaatggg tgtataaatt ctaaaatgaa tattaaaaaa acaccccgga caattaaaac
      241 tgaccgaggt attttttatt ttaatcttaa tttattttca aattactcca cgtgaatctc
      301 ttcatcacct ttatcgtaaa aacgatattc catatattga aattcgttgc tgcctgatac
      361 gtgaatttta cgtttatatt cccaatacca tttccattga atactattaa agaatctgcc
      421 ttttttaata aacgactcaa caaaaggatg tacgattaat ttaacgtatt gctgattttg
      481 atttttaacg aggtatttaa tttttctttc tatatcatca attaacaata cactagcttc
      541 aattttacct gtaccgctgc aagtagggca aacttcggta gtagtaatat ttacttcagg
      601 cttaacacgc tgacgggtaa tttgtgctac accgaatttt gaaattggta atatagtatg
      661 cttggctcta tcgttagcca tagcttcttt taatgcattg taaactgctt ttttattatc
      721 aggatgtttc atgtcgataa agtcgacaat gataattcca cccaaatccc gtaaacgcaa
      781 ttggcgggct atttctttag ccgcttcaac attaacggca acagcatttt gttcctgatc
      841 gccttttacc gcagttttat ttccgctatt tacatcaata acatgcaagg cttctgtatg
      901 ctcaacaatt aaataagcac cactatccat attaactgtt cgactaaaca gcgattttat
      961 ttgtttagtc accccaaatt gatcaaacac cgatttgcca gttgtatgga gcgttaccat
     1021 tttttcggaa ccggggctta tttttttgat atactctttt acttcatttg caattgatgg
     1081 ttcattcacc acaacattgt taaatgagtc gttgagtaca tctctcaata aggtatttgt
     1141 tttgcgtgtt tcgctgtaca caatttgcgg agcctgcagt tgttgcaaac gtttagttat
     1201 ttcagcccat tgtgcaatca aatcatttaa atcttcatgc aatagatgag tggcaactcc
     1261 ttcggcaact gtgcgaataa ttacgccaaa tccttttggt cgcaattgtt caattaatgc
     1321 ctgtagtctt tttctttcgt tggctgatgc aatttttcgt gatacaccaa caacttcatt
     1381 aaacggcgtg agaaccaggt aacgtcctgc taatgaaatt tcacaactca gtcttggtcc
     1441 tttggtttga attggctctt ttataatctg cactaaaatg tgctgatttt tggccaccac
     1501 attgttgatt ttcccggttt ttataatttc cggttctttc aagaaagaat ccatgctata
     1561 tggatgacca tcctgagcgg ctttggtata tttataaata gagcgaaaat tcgggttgag
     1621 gtccgaataa tgcaaaaacg catcgcgttc aaatccaaca tcaacaaaac aggcgtttag
     1681 gcccggcgtt atttttttca ctcttcccaa gtacaggtcg cccacattga agcggctgct
     1741 ctgagaatcg cgatggattt caacgagttg tttatcttct aataaagcaa tttcagctcc
     1801 ggttggagtt gaattaataa taagttccct ttccaaaata aatacggatt ttctcccttt
     1861 atggaattaa taaaacggtt gaggtcggaa aatataaagg gcggaaaaga tgccagttct
     1921 tatttctttt tatggcgatt ctttctcagg cgttttttac gcttgtgagt ggccatttta
     1981 tgtcttttac gtttcttacc tgatggcata ctaagtgatt ttttaatgat ttaaattatg
     2041 tatctcagtc cgcacctgat tgataatcat cgaatcggtg gtcattgatt ttagtttttc
     2101 aagttccaat atggctccgg aagtatcttt taacccaaga taagtctgag ccttcatgta
     2161 acgagcctgc aaatttaggg aatctttttg taaaactcgg tcaaatcgct ctaaagcttt
     2221 atccatttgt ccgctcatta ccgaaaaacg acccaattcc aggttcactt gtatgttatc
     2281 agggtgttgt gcatcaatct ccctaagcat cataataccc tccatagggg cattggtgcc
     2341 acttaccaaa accgttgcca ggcctatctt tgcgtctaaa tcttccggtt ttagcttgcc
     2401 tgcttcctca aaacaaaaac gggctccgta aaccacacta acacgtaaat cttcttcctg
     2461 aacaaaatct atcaggctgc ttagagcgga accggcacgc ataaaatttt cgtaggtggg
     2521 ttcactttct gctttttggc gcaggtaatc ggcagcaatc acgggctgac caatttgtaa
     2581 ccattcatct gaaagttgtt gtaataaggc agtttttgaa cttgcagagg ctttttgcat
     2641 atcgtgatgc atttccatta actgctcgcc attcgggtat tgcaatgaat ctatcagttt
     2701 cttcctttgg tcgtcaaagg cagtaactac aggtggttta ttgctaaatc caaagtaaat
     2761 tatcgctatg gctgctgcag ccaggccaac tccaatatat attcctttca ctttacaggc
     2821 ttgctttaga cttttttcct tctttaaccc tgttaatgaa tactttggca ggtttgaatg
     2881 ccggaatttt ttgctccgga atttctatgg caatattctt tttaataatc cttccgattt
     2941 tctttttgcg cgttttcact acaaaacttc caaatcccct aacgtaaacg ttattgcctt
     3001 cagccatact ttctctaatc tctttacaaa aggcttcaac aacaaccata acgtcaactt
     3061 tttcaagtcc ggttttttcc gtgatgcgat taacaatatc tgcttttctc ataggtatct
     3121 tcgttttagt ttgataatca gcactttaaa ccacaaaatt cgggatttta ttttatttac
     3181 ccgacatttt ttaacagttt gtattattgt ggtgcaatgc gtaaagtatt tacacaaaaa
     3241 ctgttccgtt ggtatagcct ttataaacga aatttgccct ggattggttc aaacgacccc
     3301 tataaggtat ggttgagcga ggtaattcta cagcagacta gggttgagca aggtttgagc
     3361 tattatcaga aatttgtcaa aaaatatcct gacattcaac aacttgccaa cgcaccacac
     3421 gacgaagtga tgaaaatgtg ggaaggattg gggtattatt cccgtgccag gaacatgcat
     3481 tccacagcta aacaagtagt tgaggaatta aatggccgtt ttcctgatac ctatgaggga
     3541 ctaattcaac taaaaggaat aggtcaatat acggctcatg ctatcctttc ctacgcctac
     3601 aaaaaaccat ttgcagttgt tgatggcaat gtattgaggg tgctggccag gtattttggt
     3661 atccgggaag caattgatac cccaaaaggg aaaagcttga ttcagcaaca tgccgatagt
     3721 ttactcgata aaaaggaacc tgatgtgttt aatcaggcga tgatggactt tggcgcatta
     3781 atttgtaaac ccaggcagcc ggattgtgaa aattgcccat tacagaaatc atgtgccggc
     3841 tatcagcagg aactcactga tatactgcct gttaaatcga aaaaaataaa acgtcagaca
     3901 aggtatttac acttttttgt atttcgtgca ggcgaaaaaa tattggttga gcaacgaatt
     3961 gcaaatgata tatggaaaaa tttatatcaa tttccggtta ttgaaacaag caaaaatgtt
     4021 tcttcaaaaa gtttaatgga aagcgaagat tatcaggcat ttcgaattaa taattctttg
     4081 ccggtaatta catctcccat ttttaaacaa caattaacgc atcagcaact tatttgcaga
     4141 ttctatattt ttgacttgaa aagtactgat aaaataaagc gctccggtgt taagcaggta
     4201 acttttacac agttaaaaca actggcattt cccggggtaa ttcgcgattt tttgaaaatt
     4261 aatgattatt tttaatgttc aaagcaaacc aaaagaaaac gaattatgag tggagtaaac
     4321 aaagtaattc tcatcggaaa tctgggtaag gacccggacc tgagaaattt tgagggcggt
     4381 aatgtggtag ctaatttcac attggcaacc accgaagttt accgcgatcg caatggcaac
     4441 aaagtagaac aaacagaatg gcacaatatt gccatgtggg ggaaattggc agaaattgcc
     4501 ggcaaaattt tgcgcaaagg tgctaaagtg tatatcgaag gtcgcattaa aaatcgttcg
     4561 tgggaggacc gcgaaggcaa taaaagatat atcactgaaa tagttgccga aaatttcact
     4621 ttattaggac cacgccctgg tggcaatggc acaggtgctc aaggtggaac tgattatgaa
     4681 atgtcgcatt caaatccgga taacgaaaaa gatatgattg tggacgacag tctgccgttt
     4741 tgacaaatag ttacacgtta tcaacttaaa acttgcaagc tctattgcaa gtttttttat
     4801 ttttaaccgt tatataatca ttatgcaaaa aaacctgttc cgattattat tagtattatt
     4861 gcccgcagca ttttttattt cctgcgaaac ggataccact cctcgaccaa tggggtttcc
     4921 aaaagtaaac tatcctattg caaaagatac tttactttac aacgacccga attgccctta
     4981 tactttttta cttcccgatt attacgaaat aaataacaag gttacatttt ttaatcaggc
     5041 tcctgaaaat ccttgttgga tgaatttaat ttgtaaagat ttaaatgcaa c
//
LOCUS       DAORKW010000236         5088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_714_length_5088_cov_5.130340, whole genome shotgun sequence.
ACCESSION   DAORKW010000236 DAORKW010000000
VERSION     DAORKW010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_714_length_5088_cov_5.130340"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..2475
                     /locus_tag="PLB46_09920"
     CDS             82..2475
                     /locus_tag="PLB46_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRF76877.1"
                     /translation="MKLFPADITQVLEFEKVLTEVAKYCDSKPGADRVQKVRPLDKFD
                     EVKKMLMQTKEMSLLLQFEEHFPHDRIFDIRETIHLMTIENYVLNEQQLNEVKKVALL
                     TGAMLRFFTTHQEKYPILYSLFTQLTYEAVIANEIKKVINDEGHIREDASPELIRIKR
                     EKDQSSRALGKVFNAALSKYRQAGFLSEVEESMRNGRRVLGILSEYKRKLSGIIHDES
                     DTGRTVFMEPEEVVNIQNEIFELEREEKREIFRILKALTTEIAPYSGLLERYQHVLGI
                     IDFTRAKGKYALEIHAEMPQLERQPVIYLYHAFHPVLYLLNKKHKKAIVPLDVELDAN
                     NRILIISGPNAGGKSVAMKTIGLLQYMTQCGLLIPAAAHSKIGIMQNIFCDIGDTQSL
                     EDELSTYSSRLIKINYFLQHSNKDTLILIDEFGTGTDPVMGGAIAEASLKALNDKKIF
                     GVVTTHYANLKAFAANNIGVFNGSMLYDEQALKPLYKLETGKPGSSYAFELAKKSNLP
                     QQLIEEARKLISKEHIRFEELLKNVRVEKEHIRLRDKEVNKKEEDLKKKEKDLELAFE
                     KAREKQERYNLKKLEKEDEAIRKMEQEFRAMLQELKNTHQQEVIPVNEKEQAKEKLRQ
                     FIQTQKKNTFGERKKIQIKQPTNYERGEIKIGGLVKLVSGSEIGVLQSVQKNRAVVIF
                     NNMKTTVPYEELIGIASADIPVKPAQVFKVTIDESEFPLELDLRGKPKEEAMMELENY
                     LDRAVMRKVFQARIMHGKGTGVIREMVQQVLKSYPGLKQYKYATREMGGDGVTEVMF"
     gene            2510..3613
                     /locus_tag="PLB46_09925"
     CDS             2510..3613
                     /locus_tag="PLB46_09925"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRF76878.1"
                     /translation="MSTALKDLYSKAFVAEISGIIKPHVKNFDEVAFAASIFDKYWKN
                     LELKQRMRHITNMLNRVLPQDFELASKVLFKISSGIQKYHGNGMHFLYMFLPDYVEQF
                     GINHFHTSVALLEKITQVTSAEFAVRPFIIKYPKPMMQQMVVWSKHDSEYVRRLSSEG
                     ARPRLPWAIALPALKKDPSPVLPILENLKNDSSETVRRSVANNLNDIAKDHPETVLRL
                     INKWQGKSKNTNWIIKHGSRTLLKQGNDEAMRIFGNHPIKSFTIQNIRSDKHVKIGGM
                     YQFQFEYLHAEKQAVDVRMDYAIYYVKSTGKISKKVFRLTEGKTHPNKLFTLTKKQHF
                     KDLTTRKHYAGEHQLAIIVNGNEVVRWKFKVIG"
     gene            complement(4077..4787)
                     /locus_tag="PLB46_09930"
     CDS             complement(4077..4787)
                     /locus_tag="PLB46_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76879.1"
                     /translation="MHEKVQIKEEFKDVTLKATCYVNITINDKGKIVKVYTPKQISTY
                     ADKDIVTAFKTMQNWEVDIAPGMTKKLLLPVKMNLVRDPSQMTIKEKTLSKPESKRYN
                     AKFESDRYIMTVGQFGWINCDKFVNSEQPRTIVYIPTDSLSDVSMTIVFHSIPSVIGG
                     LRYTSGFYFDNMPAAEKITVVAVRINEHGDTELAMKETVVDDDPITDLVFIKVTKDEM
                     IKQFEQLAKIRAPEFAEL"
     gene            complement(4830..>5088)
                     /locus_tag="PLB46_09935"
     CDS             complement(4830..>5088)
                     /locus_tag="PLB46_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76880.1"
                     /translation="LESGSMLYVAASAQDRTCNLSTNAFYEVSLPAINEEDGMQLFYS
                     DNDGKNWSPANRGAMMDFRTFNTYQPEYINPTNNPRPRKYT"
BASE COUNT         1761 a          845 c          984 g         1498 t
ORIGIN      
        1 gtgtgatttc agcgcaagat atgattttag ttggttcttt aaaaatgcgc ttattccagt
       61 acctttgtca aaaaaacaaa ggtgaaacta tttccggcag atataaccca ggtgcttgaa
      121 tttgagaagg tactcacaga ggtagccaaa tattgcgata gcaaaccggg cgccgacaga
      181 gtgcaaaagg tgaggccgtt ggataaattt gatgaagtga agaaaatgct gatgcagaca
      241 aaagagatgt cgttgttgct gcaatttgaa gagcattttc cgcatgacag aattttcgat
      301 atcagagaaa caattcattt gatgactatt gaaaactatg tcctcaatga acaacaattg
      361 aatgaagtaa aaaaagtggc tttgttaaca ggcgcaatgt tgcgtttttt cacaactcat
      421 caggaaaaat atcccattct atatagtttg tttacgcaat taacttatga agcagttatt
      481 gcgaatgaaa ttaaaaaagt aatcaacgat gaaggacata tcagagaaga tgcttcaccg
      541 gaattaattc ggataaaacg cgaaaaagac cagtcatcaa gagcacttgg aaaagtattt
      601 aatgctgcat tgagtaaata tcgccaagct ggatttttaa gtgaagtaga agaaagtatg
      661 cgtaatggca gaagagtgct tggtatttta agcgaataca aacgcaaact cagtggtatt
      721 attcatgatg aaagtgatac cgggagaaca gtcttcatgg agccggaaga agtagtgaat
      781 atccaaaacg aaatttttga attagaacga gaagaaaaac gagaaatatt tagaatatta
      841 aaagcactta ctactgaaat tgcaccgtat tcgggattac tggaaaggta tcaacatgtt
      901 ttaggtatta ttgattttac gcgggcaaaa ggaaaatacg ctttggaaat tcacgctgaa
      961 atgccacagt tggaacgaca gccagttatt tacttatatc atgcttttca tccggtttta
     1021 tatctgctta acaaaaaaca taaaaaggca attgttccat tagatgtcga attagatgcc
     1081 aataacagga tactaattat ttccggacca aatgcagggg gtaaatcagt ggccatgaaa
     1141 acgattggtt tattgcaata catgacacaa tgtggattgc ttatccctgc tgcagcgcat
     1201 tctaaaatcg gtatcatgca aaatattttt tgtgatatcg gtgatacaca atcgctagaa
     1261 gatgaattgt ctacttattc atcacgactc atcaaaatca attatttttt acaacatagc
     1321 aataaagata ccctgatact gatagatgaa tttggcacag gtactgaccc ggtaatgggc
     1381 ggtgcaattg cagaggcttc actaaaagct ttaaacgata aaaaaatatt cggagtggtg
     1441 actacccact atgcgaattt aaaagctttt gcagctaata acattggtgt atttaatggc
     1501 agtatgttgt atgacgaaca agctttaaaa cctttataca aattagaaac aggaaaacca
     1561 ggaagttcct atgcatttga gttagctaaa aaatcaaatt tgcctcaaca acttattgaa
     1621 gaagcaagaa agttaatcag caaagaacat attcgatttg aagaattatt gaaaaatgtt
     1681 cgggtggaaa aagaacatat tcgtttaaga gataaagagg taaacaaaaa agaagaagat
     1741 ttaaagaaaa aagaaaaaga cctggaactg gcttttgaaa aagcgagaga aaaacaagaa
     1801 cgttataacc tgaaaaaact ggaaaaagaa gatgaagcga tacgaaagat ggagcaggaa
     1861 ttcagagcca tgttgcagga attaaaaaat acccaccagc aagaggttat acctgtaaac
     1921 gaaaaagaac aggcaaaaga aaaattgcgt caatttattc aaacccaaaa gaaaaatact
     1981 tttggcgaac gaaaaaaaat tcagattaaa caaccaacca attatgaacg tggtgaaatt
     2041 aaaattggtg gtttagtaaa attagtttcc ggtagtgaaa ttggtgtatt acaatctgtt
     2101 caaaaaaata gggctgttgt gatatttaac aatatgaaaa ctacagttcc atatgaagaa
     2161 ctgattggga ttgcttcagc ggatataccg gtaaaacctg cacaggtgtt taaagtcact
     2221 atcgacgaaa gtgagtttcc tttagaactt gatttgcgtg gtaaacctaa agaagaggca
     2281 atgatggaat tagaaaatta cctcgaccgg gcagtaatgc ggaaagtttt tcaagccaga
     2341 attatgcacg gcaaaggcac cggtgttatt cgcgaaatgg tacaacaggt gctcaagagt
     2401 taccctggtt taaagcaata caaatatgct acacgagaaa tgggcggaga tggtgtaacc
     2461 gaagtaatgt tttaaatttc gaattctatt tataataaat tttattttca tgtctactgc
     2521 attaaaagat ttatattcaa aagcatttgt tgctgaaata tcaggcatta tcaaaccaca
     2581 tgtaaaaaac tttgatgaag tagcatttgc tgcttccata tttgataaat actggaaaaa
     2641 tctggagttg aaacagcgta tgcgacatat taccaatatg ttgaaccgtg ttttacctca
     2701 ggattttgaa ttagcatcga aagtactttt taaaatttct tccggtattc aaaaatatca
     2761 tggtaatggc atgcattttt tgtatatgtt tttacctgat tatgtggagc aatttggcat
     2821 caatcacttc catacatctg tagccttatt agaaaaaatt actcaggtca ccagtgccga
     2881 atttgctgtg cgtccattta ttattaaata cccaaaaccc atgatgcaac agatggtagt
     2941 ttggagtaaa cacgatagtg aatatgtccg cagattgagt agcgaaggtg caagaccaag
     3001 attaccatgg gcgatagcac ttccggctct taaaaaagac ccctctcctg ttttacctat
     3061 attggaaaat ttaaaaaacg actcaagtga aacagtccgc agaagtgtgg caaataattt
     3121 aaacgatatt gccaaagacc atcctgaaac tgtattgcga ttaattaata aatggcaggg
     3181 gaaatcgaaa aataccaatt ggattattaa acatggttca cgcacattgc tcaaacaagg
     3241 taatgatgag gctatgcgga tatttggcaa tcatcccata aaaagtttta ccattcaaaa
     3301 tattcgctct gataaacatg ttaaaattgg agggatgtat cagtttcaat ttgaatattt
     3361 acatgctgaa aaacaggcag tagatgtgag aatggattac gcgatttatt atgtgaaatc
     3421 aaccggaaaa attagtaaaa aagtattccg attaacggaa ggcaaaacgc atccaaataa
     3481 attattcacc cttaccaaaa aacaacattt caaagattta actacacgaa aacattatgc
     3541 aggtgaacac caactggcca taattgtaaa cggtaacgaa gttgtgcgat ggaaattcaa
     3601 ggtaattggt taacatttta acaataaatg tgcatccaat gtgctttgtc tggcagtttc
     3661 atttgcaaac cgctaattat caatagtttc cacttttgtg tttatccacc aaatgtgtat
     3721 aaaccctgtg aaataaatgt gattaaaaat gagcctttac ctattgtttt ttacaatgag
     3781 ataatatagt tttggtatac ctgacgaaaa tatccgagct cgacagcaat gaagggcaga
     3841 agtaattctg aaaggtttaa tcgcgaggtt aacacctgta ggtgaagttg caaaacaaca
     3901 cccggattcg aaagtttggt gcagatgtta caactacggt tgcaacaatt gcgaaagcgg
     3961 aattgattcg aaaagattaa ttccgctttc ttcgtaaaag tatttaacga aattaatgta
     4021 gcgctaattt cgttgatgga aaaaccctcg ccggtcatgc gagggtttta ttttttttat
     4081 aattccgcga actcaggcgc tcttatttta gcgagctgtt caaactgttt tatcatttca
     4141 tccttggtta ctttaatgaa cactaaatcg gtaattgggt catcgtctac cactgtttct
     4201 ttcatggcta gttccgtgtc accatgttca tttattctaa cagcaaccac ggttattttt
     4261 tctgcagccg gcatattatc aaaataaaaa ccggatgtgt aacgcaatcc tccaataaca
     4321 cttggtatgg aatggaaaac aatggtcatt gaaacatcgc ttaaactgtc agttgggata
     4381 tacacaatcg ttctgggttg ctcagaatta acgaatttat cgcaattaat ccatccgaac
     4441 tgacctacag tcataatata tctgtcagat tcaaatttgg cgttataccg cttgctctcg
     4501 ggtttagaca gtgttttttc tttaatagtc atctgtgaag ggtcacgaac caaattcatt
     4561 ttaacgggca acaataattt ttttgtcatc ccgggcgcaa tatctacctc ccaattttgc
     4621 atggttttaa aagccgtcac aatatcttta tcggcataag ttgaaatttg tttgggtgta
     4681 tatactttga caatttttcc tttgtcatta atcgttatat ttacgtaaca ggtagctttt
     4741 aaggtcacat ctttaaactc ctccttaatc tgtactttct catgcaaata ctcatacatt
     4801 ttttttaagc cgccgggata tgcagcctct tatgtatatt ttctgggtct ggggttattt
     4861 gtagggttaa tatattcagg ctggtaggtg ttaaatgtcc gaaaatccat catagcacct
     4921 cgatttgcag gtgaccaatt tttaccgtca ttatcagaat aaaataattg cattccgtct
     4981 tcctcgttta tagcaggcaa tgacacttcg taaaaagcat tggttgataa attgcaagtt
     5041 ctgtcttgtg ctgaagcagc aacatataac atactgcctg attccaat
//
LOCUS       DAORKW010000237         5069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_718_length_5069_cov_4.705425, whole genome shotgun sequence.
ACCESSION   DAORKW010000237 DAORKW010000000
VERSION     DAORKW010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_718_length_5069_cov_4.705425"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /locus_tag="PLB46_09940"
     CDS             <1..1573
                     /locus_tag="PLB46_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRF76881.1"
                     /translation="DGTPSIAVKNFDGVAHPVGFLYLVGNTLSTDITIIPVDGAYSQP
                     TKPNGSTDIYDGIIFKLNASGFISWSTYLGGQNGHTTITGVELDNTDNLYICGGTEGG
                     NLFPIVDPGGSAYVENTGNAFISKFANNGEPIWSTRFGVGNGDVSAERATDLTEFDGT
                     IAVVGRTDDDGFTPMATAYQDTYGGDIGDGFIIRFNSDYSFDWSTYIGLDKKDYLRTI
                     THNLEGDYFIGGNSFSDANIDAYIDATLEAGGGDLWATPQGEGDALLGKFSSEGAQLW
                     LTYYGGDNDDYISDVNFDENRNLLFVTGFTASGAPTFPINDDLPGIYTLDGTLANLSS
                     GFISAFDSDVELIYSTFIGYEVTTWGAAIYAGPNHYYNVGYESFDKFNPGEEFKPLQE
                     FDEFSDDDYYQDIPNSYVYELFGGLGDLDYEGYICMFDYAPFEGFGTSVSTIFLNDFS
                     VYPNPTEGLITVVAKNIELNNLKLTNLLGQQCPINTLNISANKIVIDLNGLESGIYYL
                     TIQDEMFTNFKIVKL"
     gene            1576..2778
                     /locus_tag="PLB46_09945"
     CDS             1576..2778
                     /locus_tag="PLB46_09945"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRF76882.1"
                     /translation="MKQLTFFLFFIISLTCCKQAACQWVKISDLPNSSFIDLYFINDT
                     IGFTIGREFIYKTTDGGLSWDTTQLGTSLDVLFEFDFYNQDTGFIVGEVSGSRAFVTY
                     DQGVNWSNIPPYAFASEIAVINPNEFLWAVSGTFGVNRYNIALGLEYAINPGIGVSDL
                     TVFSEDTIYVGGGGFARSFDSGENWEIIDMPGILWMDFPTPQIGYAAADNYFKTMDYG
                     ETWVELELPYFFDGCSSPEVVSDSIVYFPCGYDDIFDYHGIMKTIDGGVTWIFNESIP
                     PLFDDDAIGKIFCLNKDTCWCVTYTGQIYYTSNGGGETEPPVTVIENPSSNFSIYPNP
                     VVDFFTLELNESIEHGEINIVNTIGEHIPFIVTSNNGSQKVIDIKNAPPGIYLIKFGN
                     NSETIIKL"
     gene            complement(3043..4779)
                     /locus_tag="PLB46_09950"
     CDS             complement(3043..4779)
                     /locus_tag="PLB46_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76883.1"
                     /translation="MVKRINYKEFNKTKGGMREFANAVFDALGDNIFNQQSWEKLIFA
                     SLSQVRTYGKPLKENLASIERICFQINESFGIEDMKDFLKNPELTIESSALGNQAKLF
                     CKYVLGITKLPFTEYFKLESKVKNDLINISKLIDWYETNESVKTYKTNTNYKPGIDAK
                     YIDFYKRCLGQRDEILKYYLFDIKDRALKKSFYTPKWAESLFQFTETYYDRPYKFSFP
                     FYLNHFDCRKIDPIGHRLGEFYIKDGNKLAKLYKSNKLQFYKQYFKKTKFDQHFNTIE
                     YYSHHLPIHQDRKLVINELKALSKLKRWVAFYAVILPQIEGVFTEMKNIIQDENSSTD
                     GLSRKVKTVRSKYLTEDSFFDYCQYELPTLRNRFSHLGREDEYMLNSYDLLVDLAYLY
                     RLFSELDDPYLKVYKLIKKRNHENFYSIFEVTEYLELLQKINYKKKSEIKLDIETFEK
                     EFLSVECGIDYMCYELLHSFPEEYSKFELQVFQLLNRNNIVNDNPTILNTKAIQIINS
                     NESLKSDFNITFENNSNFKTLITYCSFITLQKKNLPTLNKEVKAELDAFCKIVADQIK
                     NIQFCKEVIESV"
BASE COUNT         1620 a          775 c          922 g         1752 t
ORIGIN      
        1 ggatggaact ccgagtatag cagtaaaaaa ttttgacggt gtagcccatc ctgttggctt
       61 cttgtacctt gttggaaata cgctttcaac cgatattact attatacctg ttgacggtgc
      121 ctattcgcaa ccaacaaaac ctaacgggtc gactgacatt tatgatggaa ttatttttaa
      181 acttaacgca tcaggattta tttcatggag tacttattta ggtggacaga atggacacac
      241 aacaattaca ggggttgaat tagataatac cgataattta tacatatgtg gtggcactga
      301 ggggggtaat ttatttccaa ttgtagatcc gggcggaagc gcgtatgttg aaaatacagg
      361 taatgcattt atcagcaaat ttgctaataa cggtgaaccc atttggagta cccgttttgg
      421 agttggaaat ggtgatgtat cagccgaacg agcaactgac cttactgaat ttgatggaac
      481 catagctgtt gttgggcgca ctgatgacga tggttttact cctatggcta cagcttatca
      541 agatacatat ggtggtgata ttggagatgg atttataatt aggtttaatt cggattattc
      601 atttgattgg tcaacataca tagggttaga taaaaaggac tacttacgaa caataactca
      661 taatctggaa ggtgattatt ttatcggcgg caattcattt tcagatgcta atatcgatgc
      721 atatattgat gcaacccttg aagctggtgg tggtgattta tgggctacac cccaaggcga
      781 aggtgatgca ttactaggta agttttcaag cgaaggcgct caactttggc taacatatta
      841 tggcggggat aatgatgatt atatttcaga tgttaatttt gatgaaaatc gcaatttatt
      901 atttgtaaca ggattcacag cttcaggtgc acctaccttt cccataaacg atgatttgcc
      961 aggtatttat actcttgatg gtactctagc taacctttca agtggattta tttctgcttt
     1021 tgacagtgac gtggagctaa tttactcaac atttatcggt tatgaggtaa caacatgggg
     1081 tgctgcaatc tatgcaggac ctaaccatta ttataatgta ggatatgaaa gttttgacaa
     1141 atttaatcct ggtgaagaat ttaaaccact gcaggaattt gatgaattta gtgatgatga
     1201 ttattatcag gatattccaa atagctatgt gtatgaactg ttcggcggtt tgggtgactt
     1261 agattatgaa ggttatattt gcatgtttga ctatgcacca tttgaaggat ttggaacatc
     1321 tgtaagtaca atttttctaa atgatttttc ggtttatcca aatcctactg aaggtttaat
     1381 aacagtggta gcaaaaaata ttgaattgaa taatttaaaa ctaactaatt tattaggtca
     1441 gcaatgccca atcaatacac tcaatatttc agctaataag attgtaattg atctaaacgg
     1501 acttgaaagt ggaatttatt atttaactat acaggatgaa atgtttacaa atttcaaaat
     1561 agtaaaattg taaaaatgaa acaattaaca tttttcttgt tttttatcat ctcattgacc
     1621 tgctgcaaac aagcagcatg tcaatgggta aaaatttctg atctaccaaa cagctcattt
     1681 atagatttat atttcataaa tgatactatt gggtttacca ttggacgtga atttatatat
     1741 aaaacgacag atggaggatt aagttgggac accactcaat tagggacatc tttagatgtt
     1801 ttgtttgaat ttgactttta taatcaagat accgggttca tagttggaga agtttcagga
     1861 tctcgtgcgt ttgttacgta tgatcaaggt gttaattgga gcaatattcc accttatgct
     1921 tttgcttcag aaattgcagt gataaatcca aatgaatttc tttgggctgt atctggaact
     1981 ttcggggtta atcgttataa tattgcattg ggattggagt atgcgataaa tcctggaata
     2041 ggtgtaagcg atttaacagt attttccgaa gacacaattt atgttggcgg tggcggtttt
     2101 gcccgtagtt ttgacagtgg agaaaattgg gaaataatag atatgcctgg cattctctgg
     2161 atggattttc cgaccccgca aattggatat gccgcagcag acaactattt taaaacaatg
     2221 gattatggtg aaacatgggt tgagcttgaa ttaccgtatt tttttgacgg atgcagttcc
     2281 ccagaagtag tatccgattc tattgtatat ttcccatgtg gttacgatga tatttttgat
     2341 tatcatggaa ttatgaaaac tattgatgga ggcgtaacat ggatatttaa tgagtctatt
     2401 cctcccttat ttgatgatga tgcaataggt aaaatatttt gtttaaataa ggacacatgc
     2461 tggtgcgtaa catatactgg tcagatatac tacacctcaa atggaggagg agaaactgaa
     2521 ccacctgtta ctgttattga aaatccatct tcaaattttt ccatttaccc taatcctgtc
     2581 gtggatttct ttaccttaga gttaaatgag tcaattgaac acggtgaaat taatattgtt
     2641 aatacaattg gtgaacacat cccttttatt gtgactagca ataatgggag tcaaaaagtt
     2701 attgatatta aaaatgcacc acccggaatt taccttataa aattcgggaa caattcagaa
     2761 acaataatta aattataagc ttgcatgtaa ctacttacca agggaatttt caaaaagaaa
     2821 aataaaaaag gaaaaaaaat aaaaagaaag taactgtagg attgtgttag gatataattt
     2881 gcaaggagga acggaataaa tcgttagcaa acgtgcagga gcctttgtgt ttatgccgta
     2941 gttccttgga aaattaaccg atcaaaataa ttttgtggat gaattgcatt ttttgaattt
     3001 cgagcacaaa tgtagctttt cgcaataaat agaacctctt atttataccg attcaattac
     3061 ttctttgcaa aattgaatgt ttttaatctg atcggcaact attttgcaaa atgcatctaa
     3121 ttcagctttt acttctttat ttaaggttgg caaattcttt ttttggaggg taataaacga
     3181 acaatatgtt ataagtgttt taaagttact gttattttca aaggtaatgt taaagtcaga
     3241 ttttaatgat tcattgctat ttataatttg tatagcttta gtattcaaaa ttgttggatt
     3301 atcatttact atattatttc tgtttagtaa ttgaaaaact tgtaattcaa atttagaata
     3361 ttcctctgga aaggaatgaa gtaattcata acacatataa tcaattccgc attcaacact
     3421 tagaaattct ttttcaaaag tttcaatatc taactttatt tcggatttct ttttataatt
     3481 aattttttga agcaactcca ggtattctgt tacttcaaaa attgaataaa aattttcatg
     3541 atttcttttt ttaattagtt tatatacttt taaatatggg tcatccagtt cgctaaataa
     3601 tctatatagg tatgcaagat cgactaatag atcgtagcta ttcagcatgt attcatcttc
     3661 ccttccaaga tgagaaaatc tattcctcag tgtaggcaat tcatattgac aataatcaaa
     3721 aaaagaatcc tcagtcaaat atttacttct tacagttttc actttccgac ttagaccgtc
     3781 agttgatgaa ttttcatcct gtataatatt tttcatttcc gtgaagacac cttcaatttg
     3841 gggaagaatt acggcataaa aggccaccca cctctttaat ttggataaag ctttaagctc
     3901 attaattacc agttttcgat cctggtgaat tggtaaatga tgcgaataat attctattgt
     3961 attaaaatgt tgatcaaatt tcgttttttt gaaatattgc ttataaaatt gtaatttatt
     4021 cgatttatac aattttgcaa gtttgttccc atccttaata tagaattcac ctaatctatg
     4081 accaatcgga tctatttttc tgcagtcaaa atgatttaag taaaaaggaa aagaaaattt
     4141 gtatggtcga tcataataag tttctgtgaa ttgaaataac gattcagccc acttgggagt
     4201 atagaatgat tttttcaaag ctctatcctt aatatcaaag aggtagtatt ttagaatttc
     4261 gtcacgttgg cctaaacatc gtttatagaa atcaatgtac ttcgcatcaa ttccgggttt
     4321 gtaattagtg ttagttttgt atgtttttac actttcattt gtttcgtacc aatcaattaa
     4381 tttagaaata ttaattaaat cattcttcac ttttgattcc aatttgaaat actcagtaaa
     4441 aggcaattta gtgatcccta atacatattt gcagaataat ttagcttggt taccaagagc
     4501 acttgactca atagtcagct ctggattttt taaaaaatcc ttcatgtcct caatcccaaa
     4561 gctttcattt atttgaaaac agattctttc aatggatgct aaattttctt ttaatggttt
     4621 tccgtatgta cgaacttgag atagggatgc aaaaattaat ttctcccatg actgctgatt
     4681 aaatatgtta tctcctaatg catcaaaaac tgcattagcg aattctctca ttcctccttt
     4741 tgtcttgtta aattctttgt aatttattcg cttgaccata acagcttttg aaaatcgtta
     4801 aaaaattgtg gtatttaaac aaatatataa aacgattatt taaattactt gctcgccata
     4861 aagcgtaatg aatgaagtat aacgtaatga atggagctgc gagcggagat atgggtggga
     4921 gcaaaagagc cttgccagca agcaaggcgg ttgcgaccta tgtagaagaa ggcacaagcc
     4981 ttcgcactac acaaggggtt ggcgtatgtt tttgaccaaa aaccatgaca accccgcagc
     5041 ccattcgact gcgcaggcga gctaaacta
//
LOCUS       DAORKW010000238         5055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_723_length_5055_cov_5.092400, whole genome shotgun sequence.
ACCESSION   DAORKW010000238 DAORKW010000000
VERSION     DAORKW010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_723_length_5055_cov_5.092400"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1820)
                     /locus_tag="PLB46_09955"
     CDS             complement(138..1820)
                     /locus_tag="PLB46_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HRF76884.1"
                     /translation="MHNPALKNFVKPQTATTVEGSYLSLKQVVDIAYNKKAFELTTSE
                     AVKEKVHSSYEFIQHVALNGKPIYGVNTPFGGMANQILNHDETLQLQTNLMFFLKSGA
                     GNYIGDVYVRAAMVILTNALLKGVSGIRFEIIERYAFFLQNGITPRVRELGSIGASGD
                     LVPLASVAGAVTGLADCFAVSYKGEQWGTQAISEKFRLERIKLHPKEGLALVNSTAML
                     TGIAAINLQELNKLFALSLHAHALMMQALTASVQPFDAFTHAHKPHEGQIFIAEMITG
                     LLTGSQMVRKNGDPLQGENKLYQDRYSIRCVPQFLGPIVDGFKTVQKNIEIEANSVTD
                     NPLIDMDNFHILHGGNFLGEYVAIGMDQVRNYIGLIAKHLDAQIALLVMPEFNNGLPS
                     SLSGNTTNRINMGLKGLQITGNSIMPLLLFYGNSIADKFPTHAEQFNQNINSQGFNAS
                     ILTKTSIELFRKYIAISLIFAVQAVDLRAKQMGKQVYVKDVVSPETFKTYAAIRQICN
                     KNVLPGEPLVINDNSYPMDDFIDAIDADIQYNGKLVTAANALIEKLDTYYAA"
     gene            complement(1912..3126)
                     /gene="ccrA"
                     /locus_tag="PLB46_09960"
     CDS             complement(1912..3126)
                     /gene="ccrA"
                     /locus_tag="PLB46_09960"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HRF76885.1"
                     /translation="MELVPIGQLPEIGVIPKFMFAQTLRADRFGMPISAYQQEVVPVP
                     EIGDDEVLIGVMAAGLNFNSVWAALAYPIDMLQLMQLRNENDLPYFIPGSDCAGIVYK
                     KGNNVTNVNIGDEVAIQGGWYDEQELFIRNGGDPTIGKSFRAWGYETNFGSYAQFCKV
                     KHFQCLPKPKHLSWDEAAVYMVSGVTAYRMLHHYVPHNLKQNDVVLIWGGSGGLGSMA
                     IQLVKAAGAFPVAVVNSPSKMVFCEELGAVVLNRSNYTHWGEISGEQVLPETQEIWRN
                     AAKPFLKDLLTLTNGKLPKIVIEHPGASTFPTSLYVCDKNGMVITCAGTTGYAGTFDL
                     RYLWLYNKRIQGSHYANPEECKILNNLIHEKIIHPVLGGTYSFNDLPKALQLMYDNQL
                     PCGSTAIKIGYA"
     gene            complement(3126..4679)
                     /locus_tag="PLB46_09965"
     CDS             complement(3126..4679)
                     /locus_tag="PLB46_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRF76886.1"
                     /translation="MIQLYNEILSYRAKTHPDRVALVCEDVSYTYAELYTNALKSAQW
                     LQSQQLKKGDHIGLLDFNNAAYVHIVNGCLLNGIIPVSINWRLSAREMLFILSDSNCQ
                     LFVYGNDFKQVGDEIAATNNFPVINTHECFDKIQQLTIDTTFVLPTITLSEQALIIYT
                     SGTTGNPKGVMLSHNNLFEMYYALRSETPLFGPGAVNLIAGPWYAVVGVGYFVFGVFS
                     GCTNVLLKIFDPIEILRLIQTYKITNAFFAPIMMKIICALDEVYEYDLSSLQNIQYGG
                     SPIDEEQLRICYNTFKCHFTQGYGLTETSGIATALRFDDHLRIMNSNEPELKHLLRSA
                     GKPYAGVAIKIVDEANTTLPANAIGEVCIKGNVVAMGYKNQSVINDKIFGYDGWFYTG
                     DMGYVDENGYLFLVDRKNDMIISKGQNIYPAEIEQIIIQHPAIKDIAIIGVPHEVYGE
                     AICAIVVVKKENIPLNELRNWAKDKMPEYKLPAVIEIVDELPRNPTGKVLRKLLREKY
                     WINKQRRIN"
     gene            complement(4683..>5055)
                     /locus_tag="PLB46_09970"
     CDS             complement(4683..>5055)
                     /locus_tag="PLB46_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF76887.1"
                     /translation="QQFGRWIGISSMSPYFGVPGMAIYNMQKAALENCFKTLASEEGK
                     NGITANIVAPGLILTPTVEKKYSTEELALRKKENVMHRIGSPEDIAAAVKFLASDDAG
                     YITGVTLPVNGGNHLAWQYTH"
BASE COUNT         1523 a         1009 c          805 g         1718 t
ORIGIN      
        1 acatggctgc acattgtgca accaattttt ctgtttctcc ataagtaaaa tctgttccct
       61 ggtcggtaat aaataatttt tcaccattaa ttttactttg gttgtgtagt tccctgctaa
      121 tattcataaa tacaagttca ggcagcgtaa taggtatcta atttttcaat cagtgcattt
      181 gctgcggtaa ccaattttcc gttatattga atatctgcat caatagcatc tataaaatca
      241 tccatagggt aactattgtc gttaataaca agcggctctc cgggtaatac atttttatta
      301 caaatttgtc tgatagccgc ataagtttta aatgtttcag gcgaaacaac atcttttaca
      361 taaacctgtt tacccatttg ttttgctctt aaatcaactg cctgaacggc aaatatgagt
      421 gaaatcgcaa tatatttcct aaacagttca attgaagttt tggtaagtat agatgcatta
      481 aagccctggc tgttaatatt ttgattaaac tgttctgcat gtgttggaaa tttatcagca
      541 atcgaattgc cataaaataa caacaatggc attattgaat taccggtaat ttgtaatccc
      601 tttaatccca tattaattct gttggtagta ttgcctgata atgaagaagg taaaccatta
      661 ttaaattcag gcataactaa caaagcaatt tgggcatcta aatgttttgc aattaatcct
      721 atataattgc gtacctggtc cattccaatt gctacatatt cacctaaaaa attaccgcca
      781 tgtaaaatat ggaaattatc catatcgatt agtggattat cagtaacgga gttcgcttct
      841 atttcaatat ttttttgcac tgttttaaat ccatctacaa ttggacctaa aaactgagga
      901 acgcagcgaa tagaatatct gtcctgatat aatttatttt ccccttgcag cgggtcacca
      961 tttttacgta ccatttgtga acctgttaat aagccggtaa tcatttctgc aataaatatt
     1021 tgcccttcat gtggtttatg tgcatgggta aaggcatcaa atggttgtac agaagcagtt
     1081 aatgcctgca tcattaatgc atgagcatgt agtgagagtg caaataattt attgagttcc
     1141 tgtaaattaa ttgcagcaat accagttaac attgctgtgc tatttaccaa cgctaatcct
     1201 tctttgggat gaagtttaat acgctccaat ctaaattttt cgctgatggc ttgtgtgccc
     1261 cattgttcgc ctttataact tactgcaaaa caatctgcaa gtccggtaac agctccggca
     1321 actgatgcta atggcactaa gtcgccactt gctccaatag aaccaagttc acgcacgcga
     1381 ggtgttatac cattttgtaa aaagaaagca tatcgttcaa ttatttcaaa acgaattccg
     1441 cttactcctt ttaataaggc attagttaaa attaccatgg cggcacgcac ataaacatca
     1501 ccaatataat tgcctgctcc tgattttaga aaaaacatta ggttagtttg taactgtagt
     1561 gtttcatcat gatttaaaat ctgattggcc atacctccaa aaggggtatt tacaccataa
     1621 atgggtttgc cgtttagtgc aacatgctgt atgaattcgt agctgctgtg cactttttct
     1681 ttaacggctt cactggtggt aagttcaaaa gcttttttgt tgtaggcaat atctacaact
     1741 tgctttaacg ataaataact accttcaaca gtggtagctg tttgtggttt tacaaaattt
     1801 tttaatgcgg ggttgtgcat atttgtggtt ttgggtgatg ataatgggat ttctaagtta
     1861 taaaattttt tctatatatg tatataccga gtttatatta agtctaaata attatgcata
     1921 tccaatttta atggctgtac taccacaggg taattgatta tcgtacatga gctgcaaggc
     1981 ttttggcaaa tcgttaaacg aataggtgcc acctaaaacc gggtgtatta ttttttcatg
     2041 aatcaaatta tttaagattt tacattcctc ggggttagca taatgtgagc cctgtattcg
     2101 cttattgtaa agccataaat accgcaaatc aaacgttcct gcatatcctg ttgtgcctgc
     2161 acaggtaatt accataccgt ttttatcaca tacatataat gaagttggaa aagtgctagc
     2221 ccccgggtgt tcaattacta tttttgggag cttgccattg gttaaagtca ataaatcctt
     2281 taaaaaaggt tttgcggcat tccgccaaat ttcttgtgtt tcgggaagca cttgttctcc
     2341 cgaaatttct ccccaatgtg tataattgct tctgtttaaa acaactgcgc ctaattcctc
     2401 acaaaaaacc atttttgatg gactatttac aactgctacc gggaacgctc cggctgcttt
     2461 aaccaattga atagccatag aacccaaacc acccgagccg ccccaaatta atactacatc
     2521 gttttgtttc aaattgtgcg gaacataatg gtggagcatg cgataggctg taacaccact
     2581 caccatataa actgcagcct catcccaact taaatgttta ggtttgggca gacattgaaa
     2641 atgttttact ttacaaaatt gtgcatacga accaaaattg gtttcatatc cccaggcgcg
     2701 aaaagatttt ccaattgtag gatcacctcc gttgcgaata aataattcct gttcgtcgta
     2761 ccaaccacct tgaattgcca cctcatcacc aatatttaca ttggtaacat tatttccttt
     2821 tttataaaca atacctgcgc aatcggaacc gggaataaaa taaggtaaat cattttcgtt
     2881 tcgtagttgc attaattgca acatatcaat tgggtaagct aatgcagccc atactgaatt
     2941 aaaatttaat cccgcagcca ttacaccaat caacacctca tcatcaccaa tttccggaac
     3001 tggaaccact tcctgttgat aggcagatat cggcatccca aatcggtctg cacgtaatgt
     3061 ttgtgcaaac ataaattttg gtatgacacc aatttcaggt aattgtccta tgggaaccag
     3121 ttccattagt ttattcttct ttgtttgttt atccaatatt tttcacgcaa taatttccgt
     3181 aatacttttc ctgttggatt acgtggtaat tcatctacaa tttcaattac agcaggtaat
     3241 ttgtactccg gcattttatc ttttgcccaa ttgcgtaatt cgttaagtgg tatgttctct
     3301 tttttaacaa caacaattgc acaaatagcc tccccatata cttcatgtgg tacaccaatt
     3361 atggcaatgt ctttaattgc aggatgttgt attattattt gttctatttc cgcaggataa
     3421 atattttgtc ccttggaaat tatcatatca ttttttctgt ccactaaaaa taaataccca
     3481 ttttcatcta catatcccat gtcgccggta taaaaccaac catcataacc aaatatttta
     3541 tcattaataa cagattgatt tttatatccc attgccacca catttccttt aatacatact
     3601 tcgccaatgg catttgcggg caatgtggta tttgcttcat ctacaatttt aattgctact
     3661 cctgcgtatg gttttcctgc tgaacgtaat aagtgtttta gttccggttc atttgaattc
     3721 ataattcgca gatggtcatc aaatcgcaat gccgtagcaa ttccacttgt ttctgttaat
     3781 ccatatcctt gtgtaaaatg acacttaaat gtgttgtaac aaatacgcaa ctgttcttca
     3841 tcaataggtg aaccaccgta ctgaatgttt tgtaaagaac ttaaatcata ttcgtacact
     3901 tcatccaacg cacaaattat tttcatcata attggagcaa aaaatgcatt ggtaatttta
     3961 taggtttgaa ttaaacgcaa tatttcaatc gggtcaaata tttttaacaa tacattggtg
     4021 caacctgaaa atacaccaaa cacaaaataa ccaacaccaa ctacagcata ccaaggccct
     4081 gcaattaaat taacagctcc cggtccaaat agtggtgttt cgcttcgtaa tgcataatac
     4141 atttcaaaca ggttgttgtg acttaacatt acacctttag gattaccggt tgtgcccgat
     4201 gtgtatataa ttaatgcttg ttctgataaa gtaattgttg gtaaaacaaa tgttgtatct
     4261 attgttaatt gctgaatttt atcaaaacat tcatgagtat ttattaccgg gaaattattt
     4321 gttgccgcta tctcatcacc aacctgttta aagtcgttgc cataaacaaa taattggcaa
     4381 ttgctatccg ataaaataaa caacatttct ctagcagaca atcgccagtt gatggaaaca
     4441 ggtataatgc cattcaataa acaaccattt acgatatgga cataagctgc attattaaaa
     4501 tccaataatc caatatggtc accttttttt aattgttgcg attgtaacca ttgcgcactt
     4561 tttaacgcat tggtatacaa ctccgcatag gtatacgaaa catcttcaca cacaagcgct
     4621 accctgtccg gatgtgtttt tgctctgtag cttaatattt cgttatacaa ttgtatcata
     4681 ctttaatgtg tatattgcca tgctaaatga ttacctccat ttaccggaag agtaacacct
     4741 gtaatataac cggcatcatc tgaagccaaa aattttactg ctgctgctat atcctccgga
     4801 ctaccaatgc gatgcatcac attttctttt tttctcaatg ccaattcttc tgttgaatat
     4861 tttttttcta ctgttggtgt taatattaaa cccggcgcaa cgatatttgc tgtaatacca
     4921 tttttcccct cttcacttgc caatgtttta aaacaatttt ccaaagccgc tttttgcata
     4981 ttataaattg ccattcccgg cacaccaaaa tagggtgaca tgctgctgat acctatccaa
     5041 cgaccaaatt gttgt
//
LOCUS       DAORKW010000239         5024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_730_length_5024_cov_4.480378, whole genome shotgun sequence.
ACCESSION   DAORKW010000239 DAORKW010000000
VERSION     DAORKW010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_730_length_5024_cov_4.480378"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..>611)
                     /locus_tag="PLB46_09975"
     CDS             complement(151..>611)
                     /locus_tag="PLB46_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF76888.1"
                     /translation="IIIAGHSAGGHLALYTAYNNNTDGAIKAAISLAGPTDLTADHFL
                     YTPDLTLLVENMTGTTYLSDTTAWINASPITYVNPSSPPTLLQYCGLDFTVPASQGEA
                     LAAELNTQGVEYTYHFYPIYLHDMGTVFFGGFLPDDVKTHLLTFIDANAN"
     assembly_gap    612..621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<622..998)
                     /locus_tag="PLB46_09980"
     CDS             complement(<622..998)
                     /locus_tag="PLB46_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76889.1"
                     /translation="MKKQIVFALALIGLFASCSDDDGVTGPLVEENIYEIQYGDDPQQ
                     RFDLYLPANRNSDTKILVFIHGGGWNTGDKADFTVILSALKGNDFAYANVNYRYANAA
                     IGITYEDQIADISAALDLLIAKRA"
     gene            complement(1164..2357)
                     /locus_tag="PLB46_09985"
     CDS             complement(1164..2357)
                     /locus_tag="PLB46_09985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRF76890.1"
                     /translation="MPKRKKVLHITIHLPFPANHGASLFNSYKLISYLHKNHDVFFAC
                     FLKGEDVKYKDAFLEDTGITNYYFEVLNIERNPVNLIKSYVKGITINMFRNYSSSMHR
                     KIQEIANDYDVIIAEHYEVMQYVPKNYKGKVIFRSHNAEYLIWSRYAEVETNPIKKLV
                     INAEANRIKKWELRYVAQADIVLGGTNDNEIHEPNPEKRKLKFRDYLHIGEDDQIKIP
                     IPDFDTLENSLVYVGTLTWEANIEGLIWFVQGCWTKLKQQFPELKLYIIGKNPDQRLI
                     DMSQQFQDIHITGFVVDLETYFTKCKINIIPLRFGSGMKVKTINGLCRGIPMVCTTIG
                     AEGLQVQHEKDILITDNVQTFGEYVADLLTDKNKWENIAKQSKITAAKYYTWDSLYKV
                     LDEAI"
     gene            2617..4515
                     /locus_tag="PLB46_09990"
     CDS             2617..4515
                     /locus_tag="PLB46_09990"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HRF76891.1"
                     /translation="MKKTVPNKNERLLAYSAMAAAMVAAIPDANADIVYVDIPDVTLE
                     LGDFTTAEFDGDGVIDFAFGLSSGNSGDWTFIRAFGSISFLTYGNATNMFIGYSGAIL
                     PYGSALGSGALIGPDASFVEGLYNQVFMASIYSGITYGQFGDAEDHYLGFKFDIGGEL
                     HYGWIRVNCTVGPVTLTVRDWAYDDEANTPIIAGATATPLPVANFDGDAIIVDETATT
                     TSVTVVLDMPGDCSVDLDVNDALTTATNGVDFTMSDPTVATFAGGVTTLSFDITLTDD
                     LDLEGPESIVIDINNPVGCEIGASSQYAIVIDDNEVPLPALVNMDGTAVAMSESGGSG
                     SVAINISETSDCVVDYALNAGGTTATEGSDFTYTTGSVTFVDGGALTQSFDYTIIDDA
                     EVESTEDIIFEITGVTGTCAIGLITESSVSIIDNDVAPVVGEVSLAEIAATVEEIATS
                     FTGNVTMDEASDCTVTLNVNAGASTATNGEDFTFSETVLTFTEGGATTQTFDITINPD
                     LEIEPDETVVVEITSIDGSCTLDETADALSLVIVNNDFIDINEFSNAGTQLYTFNNQL
                     MLQFDNASLAEGAGFQLLDATGRIVFDASNLVGENTFTLNTLPTGVYVARVVINGKAL
                     DKQVYLNN"
     gene            complement(4739..>5024)
                     /locus_tag="PLB46_09995"
     CDS             complement(4739..>5024)
                     /locus_tag="PLB46_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76892.1"
                     /translation="GSSNEADKPDAFGIKTERAGNWEKGGLNKGEIYFCQQICGTYME
                     QYGYEVKKISANPLIIAYYYISFPVKIALALLFNLHRMKNIGEAIKRRLK"
BASE COUNT         1559 a          884 c          919 g         1652 t
ORIGIN      
        1 agtggctgta agtggcagct caacaaaaat aaacttataa cggtgatcaa taaccatggt
       61 aattgtcgat tttcgccaca aaaataagga taaaacagct tattctctca ccgaaaatga
      121 atgctataca aaagcgatta atatgccgat ttaattggcg ttggcatcta taaatgtcag
      181 taaatgcgtt tttacatcat caggtaaaaa tccgccaaaa aacactgtac ccatatcatg
      241 taaataaatc gggtaaaagt ggtaggtata ctcaactcct tgcgtattta attccgctgc
      301 caaagcctca ccttgtgatg caggaacggt aaaatctaat ccgcaatatt gtaataaggt
      361 tggtggtgaa cttggattaa cataggtaat aggactggca tttatccagg cagttgtgtc
      421 agataaataa gtggtccctg tcatattctc aacaagtagt gtcaaatccg gcgtgtataa
      481 aaagtgatct gcggtaaggt cggttggtcc agctaaacta atggcagctt taatagcacc
      541 atcggtattg ttattatacg cagtatacaa agcgagatgt cctccggcac tgtgtcctgc
      601 aataattatc tnnnnnnnnn ngcgcgtttt gcaatcaata gatccaaggc cgcgctgata
      661 tcggctattt ggtcctcata agttatacca atagcagcat tcgcatatcg ataattgaca
      721 ttagcatagg cgaaatcatt tccttttaag gctgataata tcaccgtaaa atccgctttg
      781 tcacctgtat tccatccacc accgtgaata aaaactaaaa ttttagtgtc ggaatttcta
      841 tttgcaggca ggtataaatc aaaacgttgt tgtgggtcgt caccgtattg aatttcatat
      901 atattttctt ctactaaagg gccggtaact ccatcatcat cagaacaaga tgcgaataaa
      961 ccaattagcg caagggcaaa tacaatttgt tttttcataa ataaggcgaa atgtgtttag
     1021 gttgacagtt gaaaataatc agaatgtgtg ttttacaaaa aggtaaaata ccaattgggt
     1081 gataatttag gcacttatac ggattttcat aaaataagtt acaaaaacta ttccaaaaat
     1141 gaagtaattg gcattagagc agcttaaatg gcttcatcca acactttata taaactatcc
     1201 caggtataat attttgcggc agttatttta ctttgtttag caatattttc ccatttgttt
     1261 ttatcagtta ataaatctgc aacatactca ccaaatgttt gcacattatc agtaattaat
     1321 atatcttttt catgttgtac ttgtaaacct tctgcgccaa ttgtagtaca aaccattgga
     1381 atgccacggc ataaaccatt aatagttttt accttcatac cgcttccgaa acgcaatgga
     1441 ataatattaa ttttacattt cgtaaaatag gtttctaaat caacaacaaa acccgtaata
     1501 tgaatatcct gaaactgctg actcatatca atcagccgct ggtctgggtt tttgcctatg
     1561 atatataatt tcaactccgg aaattgttgt ttgagtttgg tccaacaacc ttgtacaaac
     1621 catattaatc cttcaatatt ggcctcccat gttaatgtgc ctacatacac caaactattt
     1681 tctaaagtat cgaaatccgg aatgggtatt ttaatttggt catcttcacc tatatgcaaa
     1741 taatcgcgga atttcagttt gcgtttttcc ggattaggtt cgtgaatttc attgtcattt
     1801 gttccaccta aaactatatc ggcttgtgct acataacgca attcccattt ttttatccta
     1861 ttagcctcag cattaattac caatttttta attggattgg tttcaacttc cgcatatcga
     1921 ctccaaatta aatattcggc attgtgtgaa cggaaaataa ctttgccttt gtaatttttg
     1981 ggaacatatt gcattacttc ataatgctct gcaattatta catcatagtc attcgcaatt
     2041 tcctgaattt tacgatgcat ggaagaggaa taatttctaa acatattaat ggttattccc
     2101 ttcacataac ttttaatcaa attgacagga ttacgttcaa tatttaatac ttcaaaataa
     2161 taattggtta taccggtatc ttccagaaat gcgtctttat atttaacatc ctcacctttt
     2221 aaaaaacaag caaaaaatac gtcgtgattt ttatgcaggt aagaaattaa tttgtacgaa
     2281 ttaaataaac ttgctccgtg gttagcaggg aaggggagat gtatggtaat atgtaatact
     2341 tttttgcgtt taggcattgg tcttgtattg aggtgttaaa atccgtccaa aattaacgcg
     2401 ttattcgata ttaacacatg tcaaaagtgt gtataaaccg tctattttag ggctatttcc
     2461 attggtttta cttccagaat tgtaaatttt aataaaaaat tggttttttt caccaaaaat
     2521 gccatttttg ccatgctaaa gtcatcttta ccccaataaa caagttaaaa cataaccatt
     2581 tctcaaactt tttaatattt tcacaaaaaa aatactatga aaaaaactgt acctaacaaa
     2641 aatgaaaggc tcctcgctta ttctgccatg gctgcggcta tggttgcagc tatacctgat
     2701 gctaatgctg atattgtata tgtagatatt cctgacgtta cgctggaatt aggtgatttt
     2761 accactgctg aatttgatgg tgacggagtt attgattttg cttttggact ttcatcaggc
     2821 aatagtggtg attggacatt tattcgcgcc tttggttcaa ttagcttttt gacctatggt
     2881 aatgctacca atatgtttat tggatatagc ggtgctattt taccttatgg cagcgcattg
     2941 ggttccggcg cattaatagg acctgatgct tcttttgtgg aaggtcttta taatcaggtg
     3001 tttatggctt ctatttattc cggaattact tatggccaat ttggcgatgc agaagatcat
     3061 tacctcggct ttaaatttga tattggtggc gaactccact atggttggat acgtgttaat
     3121 tgcacagttg ggcctgttac attgacagtt cgcgactggg cttatgatga tgaagcaaat
     3181 acgccaatta tagccggtgc aactgctaca ccacttccgg ttgcaaactt tgatggtgat
     3241 gcaattattg ttgatgaaac agctactacc acttcagtta ccgtggtatt agatatgcca
     3301 ggagattgta gtgttgattt agacgtgaat gatgcactaa caactgccac aaatggtgtt
     3361 gattttacca tgtctgaccc aactgtagcg acatttgcag gtggtgttac cacattgagt
     3421 tttgatataa ctcttaccga tgatttagat cttgaaggac cagaatcaat tgtgattgat
     3481 attaataacc ctgttggatg tgaaattggg gcttcttctc aatacgcaat tgttattgat
     3541 gataatgaag tgccgttacc tgctttggtt aatatggatg gtactgcagt tgcaatgtca
     3601 gaatcaggag gttcaggttc tgttgcaatc aatatttcag aaacatctga ttgtgttgtt
     3661 gactacgctt taaatgccgg tggaacaacc gctacagaag gttcagactt tacctatact
     3721 acaggtagtg taacttttgt cgatggaggt gcattaactc aatcatttga ttatacaata
     3781 atagatgatg cagaagttga gagtacagaa gatatcatat tcgaaattac cggcgttacc
     3841 ggaacatgtg ctattggttt aataacggag tcaagtgttt ctattataga taacgatgta
     3901 gcacctgttg taggtgaggt ttctcttgca gaaatagcag caacggtaga agaaattgct
     3961 acttctttca caggtaatgt aacaatggac gaagccagtg attgcaccgt tactttaaat
     4021 gtaaacgctg gagcatctac tgcaactaat ggcgaagatt ttacatttag tgaaacagtt
     4081 ttaacattta ctgaaggcgg ggcaactaca caaacttttg atataacaat caatcctgac
     4141 cttgaaattg aacctgacga aaccgtagtt gtagaaatta cctcaattga tggaagttgt
     4201 acgttagacg aaacagctga tgctttatcg ttagtaattg taaataacga ctttattgat
     4261 ataaatgaat ttagtaatgc aggaacacaa ttatatacat tcaataatca actcatgttg
     4321 caatttgata atgcttcatt agctgaaggt gctggtttcc agttattaga tgcgacagga
     4381 cgaattgtat ttgatgcaag taatcttgtt ggtgaaaata cctttacact aaatacactg
     4441 ccaacaggtg tttatgttgc tcgtgttgta attaacggaa aggcattgga taaacaagtt
     4501 tatttaaata attaattatc aaacaactta aaatagaagc tgcctcaaaa gggcagcttt
     4561 ttttataact gctaagactt acctcggcgc tatccacgta cggtgctggt ttttgattaa
     4621 ttcgtgaaaa tgaaaattgg taaataatgt tgggcgattg tcactagcga agtgaaaata
     4681 aattgtacta ttcgtaagga tgaaattcct gcatccagtt acattaaaaa gaaacggctt
     4741 atttcaatct acgtttaatg gcttcgccga tatttttcat tcggtgcaaa ttaaacaata
     4801 aagccaacgc aatttttaca ggaaaagaaa tataataata cgctattatt aaaggattgg
     4861 cactaatttt tttcacctca taaccatatt gctccatgta ggtgccacaa atttgctgac
     4921 aaaaataaat ttcgccttta ttgagaccac ccttttccca atttccggcg cgttcagttt
     4981 taataccaaa tgcatcaggt ttatcggctt cattactcga ccca
//
LOCUS       DAORKW010000240         5014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_731_length_5014_cov_4.176245, whole genome shotgun sequence.
ACCESSION   DAORKW010000240 DAORKW010000000
VERSION     DAORKW010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_731_length_5014_cov_4.176245"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PLB46_10000"
     CDS             complement(<1..1619)
                     /locus_tag="PLB46_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF76893.1"
                     /translation="MKSLHVTMVLAACTSFTSAMPVSAEGQITTASEATVTPLIATTF
                     LESADIDGNGILDLISGVKGNTSVQIFLNSGGTAQQLTNVKAGGNVVNLSTAVSGLYF
                     ADLNRDRLPELITASAQGTTVIVQKNQSTTGTAVFAAEQHFDIKRNAQLFFACDLNMD
                     GYQDIIAASDNNLTFYKNNCTSKAGDINLSIASEQQLAGFINAVKCADMDGDGNIDII
                     TSTSNGIYVLNGKGSGFNFDDAYAGDNYVAVSGMDIGDLDGDYLPEIVISHWPETQFS
                     ILRNISQPGSMRFTAENPITCAGANDIALCDYNQDGLFDILTLADGAGWNIAEVFRNK
                     TAAQGRFIFAAPLAISVEGNHFEISDFDNDGTDDIAAVNPLNNKVAINFNHGNSANDV
                     FNQFEAFCGEDGFTWMTWSVKKQTAKSQYIIEKTINGIDFVEVGTIVANQNLSNLNYE
                     FIVNEENSLAAGYRIKTVDENNSSHYADLILIEPCDQAVTDFVCVFPNPVDKLMNFQF
                     SVKSDLELNFEILDARMQPIMHKNASVTPGT"
     gene            1778..3325
                     /locus_tag="PLB46_10005"
     CDS             1778..3325
                     /locus_tag="PLB46_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76894.1"
                     /translation="MNYSGNIYLLIKALTKAERRYFSLQAGFNRENSHLLQVFEAIAK
                     RGFEDDASLKAWLKDTGFVGHLDVLKVHLYQLILSSMRAFYRNRNKQREIRAMVDDLH
                     FLYEKSLYSQCRKLLDKAIAAATEIGYYSALVDLLDIERMLLGVEHLEQYNETGIKQL
                     LQKELNVVTTLQSEAEFKHLGSMSGFLNKRYGHARSPEDFAALSLFTTQTKQMADKAT
                     TIRSKRLWNYSLMAFTLSTGNYEEALFYNAAIIDSYGDNQILIRENPIGLISAIGNQI
                     IICIRLKKYNDALRLLNKLHDIPQYLSKSQRRSKELMLRISLNELNYRMGIYLENGWM
                     QLAGNYMAEFELFIDTHARVIPGHRLNEMYFSVLYYNFIIGDFDKAFVYLQKLESGNL
                     QNSKADLFNSLQIIKLLMHYDLGNKHLLPYLVRSVYRMLLHLDQLYIYEQTLIRFIRK
                     NVIHVTDKKSAKKQFMWLKNELNKIQTDRYEQPAFIMFDIIAWLDSKIYEKPYGQAIA
                     DLHSQHQ"
     gene            3358..3630
                     /locus_tag="PLB46_10010"
     CDS             3358..3630
                     /locus_tag="PLB46_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76895.1"
                     /translation="MSTNTNELASKDFAQQCINSLADIKRSMPKTWKTAIQPYKDEIT
                     TTSKEKGLNSTDALLTIINKLNTDKLELSILKIKHLFFLAAYCDML"
     gene            complement(3625..3927)
                     /locus_tag="PLB46_10015"
     CDS             complement(3625..3927)
                     /locus_tag="PLB46_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76896.1"
                     /translation="MPKLHVSGITLFPFIVIKHKNLRLDKAFINHEHIHLRQQLEMLV
                     IPFYVFYLLHYLINRAKNQHHDKAYRNICFEKEAYKNESNLNYLHNRKWFAWLHYL"
     gene            complement(3968..4738)
                     /locus_tag="PLB46_10020"
     CDS             complement(3968..4738)
                     /locus_tag="PLB46_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF76897.1"
                     /translation="MSIRSSFLNRDVQLSIILPPAYEQKPYPVLWLNDGQDMSAMQFN
                     RILDDLYTQEVVKPMIVVGIHANEDRLQEYGSSAAADFKNRGAKAGLYAEFLVNELLP
                     FIRNKYSVSRDKRKNVIAGWSMGGLSAIDIAWHFSNYFGKVGVFSGSLWWRLKAYNRG
                     YKDDKHRIIHQLIRNTPKREGLRFWFECGTNDENADRNKNGLIDSIDDTIDFIQELKQ
                     KGYNEQHDIQFVIVKDGEHNQQTWAEIMPYFLTWAFGA"
BASE COUNT         1597 a          968 c          872 g         1577 t
ORIGIN      
        1 cttgtgcccg gagtaaccga tgcattttta tgcataatag gttgcattcg ggcatccaat
       61 atttcaaaat ttaattccaa atctgattta acactaaatt gaaaattcat caatttatct
      121 accggattag gaaaaacaca aacaaaatca gtaaccgcct gatcacaagg ttcaattaaa
      181 atcaaatcag cataatgact actattattt tcatcaacag ttttgatcct ataacctgct
      241 gctaaactat tctcctcatt tacaataaat tcgtaattga ggttactcaa attttgattg
      301 gcaacgatag ttcctacctc aacaaaatct ataccattta tagttttttc tataatatat
      361 tgagattttg ctgtttgttt tttaaccgac caggtcatcc acgtaaatcc atcttcacca
      421 caaaaagcct caaactgatt aaatacatca tttgcggaat taccgtgatt aaaattgata
      481 gctaccttgt tatttaaagg attaacagca gcaatatcat cagtgccatc gttatcgaag
      541 tcgcttattt caaaatgatt gccttcaacg gagatggcta gcggtgctgc aaaaataaat
      601 cggccttgtg cagcagtttt atttctaaac acttctgcaa tattccatcc tgcgccatca
      661 gcaagtgtaa gaatgtcgaa taaaccatcc tgattataat cgcataaggc aatgtcgttg
      721 gctccagcac aagtaattgg attttcggca gtaaagcgca tacttcccgg ttgtgaaata
      781 ttgcgtaaaa tactgaattg tgtttcaggc caatgactaa ttacaatttc aggtaaataa
      841 tcaccatcta aatcgccaat atccattcct gaaacagcca cgtaattatc tccggcatat
      901 gcatcgtcaa aattaaatcc actgccttta ccgttcaaaa cataaatacc atttgatgta
      961 gaagtaataa tatcaatatt gccatcacca tccatatcag cgcatttaac agcattgata
     1021 aatcctgcca attgttgctc actcgctata cttaaattaa tatcgccggc tttcgacgtg
     1081 caattgtttt tataaaaagt caggttatta tcggaggcag ctataatatc ctgataacca
     1141 tccatattga ggtcgcaagc aaaaaataac tgcgcattgc gtttaatatc aaaatgttgc
     1201 tccgctgcaa atactgctgt tccggttgtt gactgattct tttgcacaat gacagtagtc
     1261 ccttgtgcag aagctgtaat aagttccggc aatctatctc tgtttaaatc agcaaaatac
     1321 aatccggaaa cagctgttga taaattaact acattaccac cagcttttac atttgtaagt
     1381 tgctgagcag ttccaccaga gttgaggaaa atttgtacag aagtgttgcc ttttacaccc
     1441 gaaattaaat cgagtatacc attgccatcg atgtcggcgc tttctaaaaa tgtcgttgca
     1501 atcaatggcg tcacggtagc ttcggaggca gttgtaatct gcccttcagc cgagacaggc
     1561 atagcactcg taaaagaggt gcatgctgcc aaaaccatag tgacgtgtag gcttttcata
     1621 attgggttat tcgtgctttt catacttaag tcaaaattca tactaaggat tttggatgcc
     1681 aaacaaaata agccctagtt attaagtacc taattcagtt tttggctact ataatgcaca
     1741 cttttagctt aattttgcct atgaatacta agtttcaatg aattattccg ggaatattta
     1801 ccttttaata aaagccctaa ctaaagccga gcgacgttat ttcagtctgc aagccggttt
     1861 taacagggag aatagtcact tgttacaagt attcgaagcc atagccaaac gtggtttcga
     1921 ggatgatgcc agtctaaaag catggctaaa agacaccggt tttgtcgggc atttggatgt
     1981 tttgaaggtg catttatatc agttaatcct aagttccatg cgcgcttttt acaggaaccg
     2041 caataaacag cgggaaatcc gagctatggt ggatgattta cattttttat acgagaagtc
     2101 gctttacagc caatgccgca aactattgga taaggctata gcagctgcaa ccgaaatagg
     2161 ctattattca gccttggttg atttgttgga catagaacgt atgttgctcg gagttgagca
     2221 tttggagcag tataatgaaa ccggcataaa gcagttattg caaaaagaac tcaatgtggt
     2281 cactaccctc caaagtgaag ccgaatttaa acacttaggc agcatgagtg gttttttaaa
     2341 taaacgatac gggcatgcac gctctcctga agattttgca gcgctatcgt tatttaccac
     2401 acagactaaa caaatggccg ataaagccac tactattcgt tcgaagcggt tgtggaacta
     2461 tagtttgatg gcctttacct tatcgacagg taattacgag gaagccttat tttataatgc
     2521 tgccattatc gattcatatg gggataatca aatattaata cgtgaaaatc caattggatt
     2581 aatcagcgct ataggcaatc aaattattat ttgtatccgt ttaaaaaaat acaatgatgc
     2641 tttgcgctta ctcaataaac tacatgatat acctcaatat ctgagcaaat cgcaacgcag
     2701 gtcgaaggaa ttaatgttgc gcatcagcct taatgaactc aattaccgaa tgggcattta
     2761 tttagaaaac ggctggatgc agcttgcagg taattacatg gccgaatttg agctatttat
     2821 tgatacacat gccagggtta ttcccggtca tcgattaaac gaaatgtatt ttagtgtatt
     2881 gtattataat tttataatcg gagattttga taaagcattt gtttatttac aaaaattaga
     2941 gtcggggaat cttcaaaaca gcaaagcgga tttatttaat agtttgcaaa taatcaaatt
     3001 acttatgcat tatgatttag gtaataaaca tttattacct tatctggtta ggtctgttta
     3061 ccgcatgtta ttgcacttag atcaactcta catttatgag caaacactta tccgctttat
     3121 ccgcaaaaat gtaattcatg taacagataa aaaatctgcc aaaaaacagt ttatgtggtt
     3181 aaaaaatgag ttgaataaaa ttcaaaccga ccggtatgaa caacccgcat ttatcatgtt
     3241 tgatatcata gcctggttag acagtaagat ctatgaaaag ccctatgggc aggccattgc
     3301 ggacttacat tcccaacatc aataattttt tagtaccttt acatacaaac accacaaatg
     3361 agtacgaata ccaacgaatt agccagtaag gactttgcac aacaatgcat caattcgtta
     3421 gccgatatta aacgttctat gcctaaaaca tggaaaacgg ctattcaacc ttacaaagat
     3481 gaaattacca ctacttcaaa agaaaaagga cttaattcaa ccgatgcgtt gttaactatt
     3541 atcaacaaat tgaataccga taaattagaa ttatctatat taaaaattaa acatttgttt
     3601 ttcctggctg catattgtga tatgttataa ataatgcaac caggcaaacc attttcggtt
     3661 gtgcagatag tttaaattac tttcattttt atacgcttct ttttcgaaac aaatattgcg
     3721 atatgcttta tcgtgatgtt gattttttgc tcggttaatc aggtagtgca acaaataaaa
     3781 aacataaaat ggaattacca gcatctcaag ttgctgacga agatgaatat gttcgtggtt
     3841 gataaaagct ttatccagcc gtaaattttt atgtttaatt acaataaatg gaaacaaggt
     3901 tataccgctt acatgtaatt tcggcatttg aataaattgc caatttttaa ttttacccgg
     3961 catgttttta tgcaccaaat gcccaagtga gaaaataagg cattatttcg gcccaggttt
     4021 gttgattatg ttcaccatct tttacaatta caaattggat atcatgttgt tcgttatatc
     4081 ctttttgttt gagttcctga ataaagtcga tggtatcatc aatagaatca attaatccat
     4141 ttttattcct gtccgcgttt tcatcattag tgccacattc gaaccaaaac cttaaacctt
     4201 cgcgtttagg tgtatttctg atgagttgat gaatgatgcg atgtttatcg tctttatatc
     4261 cgcggttata cgcctttaaa cgccaccata agctgccact aaaaacgccc actttgccaa
     4321 aatagttaga aaaatgccag gcgatatcga tggcacttaa tccacccata ctccaaccgg
     4381 caattacatt tttacgttta tcgcgggaaa cactgtattt attgcgaata aacggtagta
     4441 actcgttgac taaaaattca gcatagagac ctgcttttgc gcctctgttt ttaaaatcgg
     4501 ctgcagcact gcttccatac tcttgtaatc tgtcttcatt ggcatgaata cccaccacaa
     4561 tcataggctt cactacttcc tgggtatata aatcgtctaa aatgcgatta aactgcatgg
     4621 cagacatatc ctgtccgtcg tttagccaaa gcacaggata gggtttttgc tcataggcag
     4681 gcggcagtat gatagaaagc tgaacatccc tgttaaggaa tgaagatcgg atgctcaagg
     4741 attcgatttt tacgcgttct ggaacctgca acttcatttt cacgtacaaa tgtacatcaa
     4801 accaataaaa taattttttg taaacagacg cattctgtcg ataactgatt gggagttggt
     4861 tgcttttgaa ctacctttgc tgctgttttt taaaccgccc taaatcaact attttggaac
     4921 ttaacgaaca aaagcacaaa tggcattcca agttacttgg aattgatatg gaaatgttaa
     4981 cctttggcac caaaggattt ccgcttatta tttt
//
LOCUS       DAORKW010000241         5005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_733_length_5005_cov_4.218990, whole genome shotgun sequence.
ACCESSION   DAORKW010000241 DAORKW010000000
VERSION     DAORKW010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_733_length_5005_cov_4.218990"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..993)
                     /locus_tag="PLB46_10025"
     CDS             complement(10..993)
                     /locus_tag="PLB46_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76898.1"
                     /translation="MLIPKVVLLALSLPIVNMIKSTSYFCLVALFIAAITLSSCNKDV
                     RTFSDMHFSFVFDETQERLDNFGYPATIPVGHAAQTPEFSLMSVHYIELSQDAFTPFK
                     DGTILYMAPETDAGGESAINFDSSLLANENTDFLKVNLERLQPGTYLYARVSVAYQQY
                     TVDYNLNNVPVIGNLPNQRGTVASFIGYRTYIGDLKIENLTTTINANKAQGFWGFETL
                     FTDGLADYNAIYTGQAPAGATTVVNPIDATSPVPAGSCVITGAFTEPLVINGDETGEL
                     YISLSFSNNQSFEWVDTNGNGQWDIDATNPENTEAVTDMGLRGMIPSWEWK"
     gene            complement(1000..3264)
                     /locus_tag="PLB46_10030"
     CDS             complement(1000..3264)
                     /locus_tag="PLB46_10030"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRF76899.1"
                     /translation="MKFNIWKWVAGMSPKIAIGIFSILTTAAIYFSFQLTFSYDYEDF
                     FPKGDKELDFYYLFRDKFEHDDNFLLIGLQPEAGVFDANFLAKLDSITQQTQRLESAE
                     KVFSITNYNYYLKTPFGFVDYPAVHIQDSSRYAEDSTRLKLDERVNGKLISQDFTTTV
                     VLIKTPDTLSQIQSEHLIAGVKNIMADLQVEDFHLLGKANFQVELIRIQQKEFTLYAL
                     LSFLLVGFVTYLMFRKWWCVFIAMLTVGIALIVFTGILGASGIEQNVMSTLFPIVIII
                     IGISDAVHFIGKYIVELRKNANKNIALYRTLKDIGFATFLTAVTSAIGFATMLTSNVP
                     PIKFFGILSALGVLIVYFVVLFFISPVLKLFSLAQLDNNASGENKKWYNFLHYVYLSG
                     KNYPRRILLISGCILLIFTYGITQISTDIHLEAGMPKNERVTTDFNFFETHFNGFRPF
                     EIAAVAQNGYKITDPVVLQTIEQVEQYAKQYDIINGVQSITMIYKSMHRAYNGDNPVA
                     YQLPEDTMVLQQYNRDLSRIKSSEMHLLLSEDKRYGRISATLSDAGTDSIRAVQNDIQ
                     QFINKLPNQDKVQFTITGTGIIFDKNTQYLRRNIISGVLLAFLCIGIVMAFMFRDWKM
                     VLISIIPNILPLAVCAGIMGLLKIELDAPTSIIFGISYGIAVDDTIHFLSKFRIERLK
                     GHDVETAIRNTFEETGKAVFIMSVILFFGFSILMLSPTAATFNIGLLTGITLFSAVWP
                     DVYLLPIMLRKWMK"
     gene            3566..3952
                     /locus_tag="PLB46_10035"
     CDS             3566..3952
                     /locus_tag="PLB46_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76900.1"
                     /translation="MKQLLQKLLFSLLLVSAVSLTGWSQSPVSGKAVISQELIITLDE
                     TAPLSTQYVFSVEGLNLKDAASAEYFFLLCHDNTAHFSVDYIKREATVYLQEGVRPKT
                     FTVADYNAYFAQIAARYTRMWNVVYN"
     gene            4075..>5005
                     /locus_tag="PLB46_10040"
     CDS             4075..>5005
                     /locus_tag="PLB46_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76901.1"
                     /translation="MKGNLVEVGVNECGAYGSNAAPPAGYHPNVGTGLGFIADSNEPG
                     WEEGSPWGYCGDYFVPGAPVEGWQLQIGGDVWTNTDQFCSPFSIDGSVASYTFAAGLY
                     TTVWEGEIPSENLSVTQITTLPEDALYFVTRVLLCNDGPTAINEIYYKRNVDPDNEAA
                     WTGEGFVTDNTIVYQPPVDEDALVSAVSSPMGCYLGMGARDLNSRVSWGSFATDGGTP
                     ENAWNGTGPYSNDGMITEDGATQIAFYIPTILPGECKCIAFAYILNEDDLAEALDATV
                     TYNVTAGGDIIASGDSSAVCNSGDTISLEVIGAN"
BASE COUNT         1582 a          937 c          877 g         1609 t
ORIGIN      
        1 aataatttct tacttccact cccaacttgg aatcattcca cgtaggccca tgtcggtaac
       61 cgcttctgta ttctcggggt ttgtagcatc tatatcccat tgaccattgc catttgtatc
      121 cacccattcg aaactctggt tatttgaaaa tgataggctg atatataact ctcctgtttc
      181 atcgccatta attactaaag gttcggtaaa tgctcccgtt attacacaag agcctgccgg
      241 caccggtgag gttgcatcaa tgggattaac aacagtagtt gcacctgccg gagcctgccc
      301 ggtatatatt gcattgtaat cggctaaacc atcagtaaat aaagtctcaa agccccaaaa
      361 gccctgtgct ttatttgcgt taatagttgt agtaagattt tctattttca ggtcgccaat
      421 ataggtccta tatcctataa aagatgcaac agttcctcgt tgatttggta aattaccaat
      481 aacaggaaca ttattaaggt tgtaatcaac tgtgtattgc tggtatgcca ccgaaacacg
      541 cgcatataag tatgttcctg gttgtaaacg ttcaagattt acctttaaaa aatctgtatt
      601 ttcattggct aataatgaag aatcaaaatt tattgcactt tcaccacctg catccgtttc
      661 gggtgccata tacaaaattg tgccgtcttt aaatggagta aatgcatctt gagatagttc
      721 tatatagtga acactcatca aagaaaattc aggagtttgt gcagcatggc caaccggtat
      781 agtagcagga tagccaaagt tatctaaacg ctcctgcgtt tcatcaaaaa caaaactgaa
      841 atgcatatcg ctgaacgttc taacatcttt attgcaagac gataatgtga tagcagctat
      901 aaataaagct actaaacaaa aataggaggt gctttttatc atatttacaa taggtaaaga
      961 taaggcaagt aaaacaactt taggaattaa caaagggaat tatttcatcc actttctgag
     1021 catgattggc aataaataca catcaggcca aactgctgaa aaaagtgtaa tccctgtcaa
     1081 taatccaatg ttaaatgttg cagcagttgg tgataacatt aaaattgaaa aaccgaaaaa
     1141 taatattact gacataataa acacggcttt gccggtttcc tcaaaagtgt ttcggattgc
     1201 cgtttcaaca tcgtgacctt tcaatcgttc gattctgaat ttcgataaaa aatggatggt
     1261 atcgtctact gcaattccat acgaaatacc aaaaattatt gaagttgggg catctaattc
     1321 tatctttaat aatcccataa ttccggcaca aactgctaat ggcaaaatat tgggaataat
     1381 actaatcaaa accattttcc aatcccgaaa cataaacgcc atcacaatac ctatacataa
     1441 aaatgccaat aacacaccac tgatgatatt acgccgtaaa tattgtgtgt ttttatcaaa
     1501 aataattccg gttcctgtta tggtaaattg tactttgtcc tgattgggca acttattaat
     1561 aaattgttgg atatcatttt gcaccgcacg aatactatct gtgcctgcat cactcaatgt
     1621 ggcgctaatt cgcccatacc gcttatcctc cgataacaat aaatgcattt cggaagactt
     1681 gatccggctt aaatcgcgat tgtactgttg caacaccatt gtgtcttccg gtaattgata
     1741 agcaacagga ttatccccat tataggctcg atgcatactt ttgtatatca ttgttatact
     1801 ttgtacaccg ttgataatat catattgttt cgcgtactgt tcaacttgtt caatagtttg
     1861 caacactacc gggtcggtta tcttatatcc gttttgtgca acagctgcaa tttcgaaagg
     1921 acggaaacca ttaaagtgtg tttcgaaaaa attaaaatca gtggtgacac gttcgttttt
     1981 tggcatacct gcttccagat gtatgtcggt actgatttga gtaataccat aagtaaaaat
     2041 caataatata caaccgctaa ttaataaaat tctgcgggga taatttttac ctgaaagata
     2101 aacataatgt aaaaaattat accatttttt attttcaccg gatgcattat tgtctaattg
     2161 tgctagtgaa aataatttca gcacagggct tataaaaaac aatacaacaa aatatacaat
     2221 taacacacca agtgcagata atataccaaa aaatttaata ggaggtacat tagaagttaa
     2281 catggtagcg aaaccaattg ctgaagtaac agcggttaaa aatgtagcaa atccaatgtc
     2341 ctttaaagtg cgatataatg caatattttt attcgcattt tttcttaact caacgatgta
     2401 tttaccaata aaatgtacgg catcagaaat cccgattata ataattacaa taggaaataa
     2461 tgtgctcatc acattctgct caatccctga tgcgcctagt attccggtga atactattag
     2521 tgcaattcca accgtaagca tagcaataaa aacacaccac cattttcgaa acataagata
     2581 ggtgacaaat ccaaccaata aaaaactcag taatgcatat aaggtaaatt ctttttgttg
     2641 aatacgaatt aattccacct gaaaattagc tttacctaat aaatgaaaat cttcaacttg
     2701 taaatcagcc atgatgtttt ttacacccgc aattaaatgt tcagattgaa tttgcgaaag
     2761 cgtatcagga gttttaatta atacaactgt agtggtaaaa tcctgggaaa ttaattttcc
     2821 gtttacccgt tcatctaact ttaatcgtgt actgtcttca gcataacgtg aagagtcttg
     2881 aatatgtacc gcagggtaat ctacaaaacc aaaaggtgtt tttaaataat aattatagtt
     2941 agtaattgaa aaaacttttt cagctgactc gagtctttgc gtttgttgtg taatactatc
     3001 caacttggct aaaaaattgg catcaaaaac accggcctcg ggttgaagtc ctatgagtaa
     3061 aaaattatca tcgtgttcaa atttatctcg aaacaaataa taaaaatcca actctttatc
     3121 accttttgga aagaaatctt cataatcgta agaaaaggtt aattgaaaac taaagtaaat
     3181 ggctgctgta gttaaaatac taaaaatgcc aattgcaatt tttggtgaca tccccgctac
     3241 ccacttccaa atgttaaatt tcatattcgc attttttcgg ctgcaatttc tgcttgttga
     3301 atttatcaga cactttactc atgttgaaat ccactcaaaa tgtcgccgct ttctttaaaa
     3361 aacttacttt ttacaataaa ctgacattta cagttactcg ctgttattca accatatacg
     3421 taagttttta ggctttgtag cataacaaat gcaaaaaaag cggttattgt tcataagtga
     3481 ccgggtcaaa tttgcatttt tactactcat gtcctacctt tataaagatt ttttttaacc
     3541 aattaaatat atacgaaaca tgtttatgaa gcaattatta caaaagctgc tgttttcact
     3601 cctccttgtt tcggctgtat ctcttaccgg ttggtctcaa tctccggtta gcgggaaagc
     3661 tgttatttct caggaattaa ttatcaccct cgatgaaact gcaccacttt caacacaata
     3721 cgtgttttct gttgaaggct taaacctcaa ggatgctgct tctgcagagt atttcttttt
     3781 gttgtgtcat gataacacag cgcatttttc tgttgattat atcaaacgtg aggcaacagt
     3841 ttatttgcag gaaggagtaa ggcctaaaac ctttaccgta gctgattata atgcttattt
     3901 cgcgcagatt gctgcacgtt ataccagaat gtggaatgta gtttataatt aattttttaa
     3961 ttctttaatt tacttgaaat gcatagcaac aaatacgttg tttctttatt gatatttact
     4021 ttgctaattc tatcaattag cacaaaagcc caaattgttg gtgttaattg ttttatgaaa
     4081 ggtaaccttg ttgaagtagg ggttaacgaa tgtggtgctt atggctctaa cgctgcacct
     4141 cctgccggct accatcccaa cgtgggtaca ggtttaggtt tcattgccga ctctaatgaa
     4201 ccaggttggg aagaagggtc tccatggggc tattgtggcg attattttgt tccgggtgca
     4261 cctgtggaag gttggcaatt acaaattggc ggtgatgttt ggacaaatac cgatcaattt
     4321 tgttcaccat ttagcattga tggttctgtt gcctcttata ctttcgcagc agggctttat
     4381 actacagtat gggagggaga aattcctagt gaaaatttga gcgtaactca aatcactacc
     4441 ttacctgaag atgcccttta ttttgtaacc cgtgtattgc tttgtaatga cggacctaca
     4501 gcaataaatg aaatttatta taaacgcaat gtggatcctg acaatgaagc agcatggact
     4561 ggagaaggct ttgtaactga taataccatt gtttatcaac cacctgtaga cgaagatgct
     4621 ttggtttctg ctgtaagttc tccgatgggt tgttatttag gtatgggcgc acgtgattta
     4681 aattcaagag ttagttgggg ctcatttgca actgatggtg gtacccctga aaatgcctgg
     4741 aacggaaccg gtccatattc aaatgatggc atgattaccg aagatggtgc cactcaaatt
     4801 gctttttata ttcctacaat tttacccggt gaatgtaaat gtatagcatt cgcttatatc
     4861 ttaaatgaag atgatttagc agaagctcta gatgctacag ttacctacaa tgttactgca
     4921 ggtggcgata ttattgcctc cggcgactca tctgctgttt gtaactccgg tgatacaatt
     4981 tccttagaag taattggtgc taatg
//
LOCUS       DAORKW010000242         5000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_735_length_5000_cov_5.792720, whole genome shotgun sequence.
ACCESSION   DAORKW010000242 DAORKW010000000
VERSION     DAORKW010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_735_length_5000_cov_5.792720"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PLB46_10045"
     CDS             <1..675
                     /locus_tag="PLB46_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRF76902.1"
                     /translation="AYYQPVIIILSAIAIIYGAFATLMQTDLKYINAYSSVSHCGFVL
                     LGIGMLNQTAINGAVLQMVSHGLMTALFFAAIGMVYDRTHTRMVKDIGGLLKVMPFIA
                     TVFILAGLCSLGLPGLSGFVAEMTVFMGSWQNPETLYRVATILACASIVVTAVYILRA
                     AGKTMFGPIVNQEHLSFTDAAWNERLAAIVLLAAILIMGIAPFVVTDIIYPGTEVMMQ
                     KIIVHQ"
     gene            729..2138
                     /locus_tag="PLB46_10050"
     CDS             729..2138
                     /locus_tag="PLB46_10050"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRF76903.1"
                     /translation="MTSELLLLMKFELMVTIILFIILLLRLGKNVMATSTLLNLVNIL
                     LAVNFIAGFFSNTVGDAFGSMFHSTQLIHLEKNILNLAVLVISLQSREWLSSHKHIHE
                     FYVILLASLSGMFFMISAQNMFMFYLGLELSTIPLAALANFDLAKRKSGEAAMKFIIS
                     SAFSSGILLFGISLLYGALGTLSYEGIAATMSGQPLLILAFVMILAGFAFKMSAVPFH
                     LWTADVYEGAPVAVTAYLSVVSKGAVIFVFFSVLYTAFEHIFISWYNFLFLISVLTMI
                     TGNLFALRQNNIKRFLAFSSIAQVGFILIGITGNSAEGAAATIFFILIYLFSNLGAFG
                     VISLVSAKTGKENISDYKGFHKTNPLLAWILALSLFSLAGIPPTAGFFGKFFLLMSGA
                     AQNNTALIVIAALNMIVSLYYYLKVVKAMFMDSNEMAIPKVNTGFQAGLAFAICMAGL
                     LITGLFGGCYEYIFSIIHL"
     gene            2150..2515
                     /locus_tag="PLB46_10055"
     CDS             2150..2515
                     /locus_tag="PLB46_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76904.1"
                     /translation="MAINQNHTFDDLDGIKCAIVEKNAAKERVDFLKDLLTFNGFTVV
                     VVPTPPAKGAPVPAEGEVPPPPTSWTVGVSDATFNPTNAIYGRLLRTRTGKVVTMAYW
                     QQKDTESHDQIPYFAKTWR"
     gene            2588..>5000
                     /gene="pheT"
                     /locus_tag="PLB46_10060"
     CDS             2588..>5000
                     /gene="pheT"
                     /locus_tag="PLB46_10060"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRF76905.1"
                     /translation="MKISWNWLNELLPLSMTANEAAVLLTDIGLEVEGVYPFSTINGG
                     LEGLVVGEVMSCEKHADADKLKVTRVNIGTDELLQIVCGAPNVAVGQKVIVAIPGTTI
                     YPIKGEPFTIKKAKIRGVESSGMLCAEDEIGIGESHDGLKILPENSTPGTAVKTLFDV
                     YNDTIIEIGLTANHADANSHTGTARELRAALISRGLGKPELISTQQNNISVNSLPAAI
                     KVQVENTAACPRYSGVLLKNIVVGESPKWLQNKLRAIGMRPINNVVDITNYILQLYGQ
                     PLHAFDADKIEGNTIIVKTVPQNTTFITLDGKERKLSEQDLMICDANGGMCIAGVYGG
                     LHSGVTTTTQQVFLESAYFNPSYIRRTESVHGLKTDASARFAKGTDIESTVVALQHAI
                     SLIQSICGGEIAGDIVDIYPNKMPEYQVNLRLSRLAKISTLQLPTANIITILEALNIT
                     VAQQTDDVLQLKVPTYKNDVIREIDIIEEILRMHGFNNIQVPATIKTPYLVSPKPDKE
                     KVKLETINYLVSTGFYEIFTNAISRSKYAQNYLPEFAPKQVNLLNSLNAELDSMRLTM
                     AFTGLEVIGYNSNHKQTDLALFEFGRVYSKSETYQEAEKIGMFISGNKYLENWKIINR
                     KTDFYDIKAAVDALLNKMNCKGDWSPIGNDNQNDLSPLFEQGATISVGGKVIGCCGSI
                     KKQILADFDIKQPVYFAELDWNMMLEKMASRKVTFKEITKFPEVRRDLALVIDKSVNY
                     DAIAAIAKKEGKPILKDLILFDVYEGEKLEGKKSYAIGLTFSNDERTLTDSDVDGVMK
                     KLMTRC"
BASE COUNT         1599 a          856 c          949 g         1596 t
ORIGIN      
        1 gcttattacc aaccggttat tattatttta tcggccattg ccattattta cggcgcattt
       61 gctacgctga tgcaaaccga tttaaaatat atcaatgcgt attcgtccgt ttcacattgt
      121 ggttttgtgt tattaggaat aggcatgttg aatcaaacag caattaatgg tgcagtgtta
      181 caaatggtat cacacggttt aatgacagcc cttttctttg ctgctatcgg aatggtttac
      241 gaccgcacac ataccagaat ggtaaaagat attggtggtt tattaaaagt aatgccattt
      301 attgctaccg tatttattct tgcaggttta tgttcattag gcttgcctgg tttaagtggg
      361 tttgtagcag aaatgaccgt atttatgggt agctggcaaa atccggaaac cttatatcgg
      421 gtagcaacaa ttttagcttg tgcaagtata gtggtgacag cagtttatat tttacgcgcc
      481 gcaggcaaaa caatgtttgg tccaattgtt aatcaggaac atttaagttt tacagatgcc
      541 gcctggaacg aacgtttagc tgccatagta ttattagctg caattttaat tatgggtatt
      601 gccccatttg tggttactga tattatttat ccgggaacgg aagttatgat gcaaaaaata
      661 atagtacatc aataaataaa atagcattca atcagcatga catttactga tgtcgaaaca
      721 gaaaacatat gacgagtgaa ttactactac taatgaaatt tgagttgatg gttaccatca
      781 tcctgttcat tattttacta ttaaggttag gtaaaaatgt aatggccaca tctacattac
      841 tcaaccttgt gaatatatta ctcgctgtaa attttattgc cggatttttc agcaatacag
      901 tcggtgatgc atttggcagc atgtttcata gcacccaatt aatccacctc gaaaaaaaca
      961 ttctcaactt agccgtttta gttatttcat tgcaatctcg tgaatggctc agttcacata
     1021 aacacatcca tgagttttat gttattttat tagcttcttt gtcaggaatg ttttttatga
     1081 tttctgccca aaatatgttt atgttttatt tagggctgga actatcaacc attccacttg
     1141 cagcattagc caacttcgat ttagcaaaac gcaaatcagg tgaagctgca atgaagttta
     1201 ttatttcatc ggcattttca tcaggtatat tattatttgg catctcatta ctttacggtg
     1261 cgcttggtac tttaagttat gaaggtatag cagctaccat gagtggtcag ccgttactca
     1321 tattagcctt tgtaatgata ttagcaggct ttgcatttaa aatgagtgct gttcctttcc
     1381 acttatggac ggcagatgtt tacgaaggag cacctgtggc agtaactgcc tatttaagtg
     1441 tagtttctaa aggcgcagtc atttttgtat tctttagtgt gttatatact gcattcgaac
     1501 atatttttat ttcctggtat aatttcctgt ttttgataag tgtgctcact atgattacag
     1561 gtaatttatt tgcattaagg caaaacaata ttaaacgatt tttagccttt tcatcaattg
     1621 ctcaggttgg atttatttta atcggtatta caggtaattc tgcagaaggt gcagcagcta
     1681 ccatattttt tattctgatt tatttatttt ccaatttagg tgcgtttggt gttatttcat
     1741 tggtaagtgc taaaacagga aaagaaaata tttcggatta taaagggttt cataaaacca
     1801 atcctttgtt agcatggatt ttagcgttgt cattattttc acttgcaggt attccaccta
     1861 cagccggatt tttcggaaaa ttctttttac tcatgtcggg tgctgcacaa aacaatacgg
     1921 cactcatcgt aatagcagca ctcaatatga ttgtttcatt gtattattat ctcaaagtag
     1981 ttaaagccat gtttatggat agcaacgaaa tggcaattcc taaagtgaat accggatttc
     2041 aagcaggtct cgcttttgca atttgtatgg ctgggttatt aatcaccggt ttatttggcg
     2101 gttgttacga atatattttt tcaatcattc atttataaat agtaagtaaa tggcaattaa
     2161 tcaaaatcac accttcgacg atctggatgg tataaaatgc gctattgtag aaaaaaacgc
     2221 tgctaaagaa cgtgtagatt ttttaaaaga cttactaact tttaacgggt ttaccgttgt
     2281 agtagtacct acaccaccgg caaaaggcgc tccagtgcct gctgaaggtg aagtacctcc
     2341 tcccccaaca tcctggaccg ttggtgtttc ggatgccact tttaatccta ccaatgccat
     2401 ttacggcagg ctactacgca cgcgcaccgg taaagtggta actatggcct actggcagca
     2461 aaaggatact gagtcgcacg accagatacc ctattttgcc aaaacctggc gataatttta
     2521 agtcagctgc tcctattatc ttccaaatta tatagcctta acgcaactgt tattaaattt
     2581 gcgggatatg aaaatatcct ggaactggct caatgaatta ttaccactca gcatgacagc
     2641 aaatgaagct gccgttctgt taactgatat cggattagaa gtagaaggtg tatatccatt
     2701 ttcaactatt aacggaggcc ttgaaggact agttgttggc gaagtaatga gctgcgaaaa
     2761 acacgcagat gccgataaat taaaagtaac tagggtaaat attggtacag atgaattact
     2821 acaaattgtt tgcggtgcac caaacgttgc tgtcggacaa aaagtaattg ttgccattcc
     2881 cggaactacc atttacccaa ttaaaggaga gccatttact ataaaaaaag caaaaatacg
     2941 cggtgttgaa agcagcggta tgttatgtgc cgaagatgaa attggtattg gcgaaagtca
     3001 cgatggttta aaaattttac cggaaaattc cactccagga acagccgtta aaactttatt
     3061 tgacgtttac aatgatacaa taatcgaaat tggattaact gccaatcatg ctgatgccaa
     3121 ttcacatacc ggtaccgcaa gagaattgcg tgctgcatta atctcacgtg gtttaggtaa
     3181 accggaatta atatctacac aacaaaataa tatctctgta aattcacttc ctgctgctat
     3241 aaaggttcag gttgaaaata ctgcagcatg tcctcgatat tcgggtgtat tattaaaaaa
     3301 tattgttgtt ggtgaatcac caaaatggtt gcaaaataaa ttgcgtgcaa ttggtatgcg
     3361 tccaattaat aatgttgtag atattactaa ttatatttta caactctacg gtcaaccatt
     3421 acatgcattt gacgcagata aaattgaagg taataccatc attgtaaaaa ctgttccaca
     3481 aaacacaaca tttattacac ttgatggtaa agaaagaaaa ttaagtgaac aagatttaat
     3541 gatttgtgat gccaatggcg gtatgtgtat tgcaggtgtt tatggcggtt tacattccgg
     3601 tgtaacaact acaacacaac aagttttttt agaaagtgct tattttaatc cttcgtatat
     3661 cagaaggact gaatcggtgc atggattaaa aactgatgct tctgcacgtt tcgcaaaagg
     3721 aacagatatc gaatcaactg ttgtcgcatt gcaacatgct atatcattaa ttcaatcaat
     3781 ttgtggtggt gaaattgccg gagacattgt tgacatctac ccaaataaaa tgcctgaata
     3841 ccaggtaaac ctgcgcttaa gtcgtttagc taaaataagc acattacaat tacctacagc
     3901 aaacatcatt accattctgg aagcactgaa tattactgta gctcaacaaa cagatgatgt
     3961 tttacaattg aaagtgccta catataaaaa tgatgtaatt cgtgaaatag acattattga
     4021 agaaatattg cgcatgcatg gatttaataa tattcaagtt ccggcaacca taaaaactcc
     4081 ttatctggta agtcctaaac cggataaaga aaaagtgaaa ctggaaacaa ttaattattt
     4141 agtgagcact ggtttttatg aaatatttac caatgccatt tcacgttcta aatatgctca
     4201 aaattattta ccagaatttg caccaaaaca ggtaaactta ttaaattctt taaatgctga
     4261 attggatagc atgcgattaa ccatggcatt taccggactt gaagtaattg gatataatag
     4321 caatcacaaa caaactgatt tagctttatt tgaatttggc agagtataca gtaaatcgga
     4381 aacttatcag gaagctgaaa aaataggcat gtttatttca ggtaataaat atctcgaaaa
     4441 ttggaaaata atcaatcgca aaacggactt ttacgatatt aaagcagctg ttgatgcttt
     4501 attaaataaa atgaattgta aaggtgattg gtcaccaatt ggcaatgata atcaaaatga
     4561 tttatcacca ctttttgagc aaggtgcaac aatttcagtg ggtggaaaag taataggctg
     4621 ctgcggtagt attaaaaaac aaatactcgc cgattttgat attaaacaac ctgtttattt
     4681 tgccgaactg gattggaata tgatgctgga aaaaatggca tccagaaaag tgacctttaa
     4741 agaaataact aaatttccgg aagttcgccg cgatttagca ttggtaatcg acaaatcggt
     4801 taactacgat gctattgccg ccatcgctaa aaaagagggc aaaccgatat taaaagattt
     4861 gattctgttt gacgtttacg aaggcgaaaa actggaaggc aaaaaaagtt atgctattgg
     4921 attaaccttt agcaacgacg aacggacttt aaccgattcg gatgtagatg gtgttatgaa
     4981 aaaattgatg acgcgttgcg
//
LOCUS       DAORKW010000243         4985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_739_length_4985_cov_4.707099, whole genome shotgun sequence.
ACCESSION   DAORKW010000243 DAORKW010000000
VERSION     DAORKW010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_739_length_4985_cov_4.707099"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PLB46_10065"
     CDS             complement(<1..818)
                     /locus_tag="PLB46_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76906.1"
                     /translation="MKKTYIKISAAAMLIIGSATLSNAQIVGGVSYLKGKWVEVAISD
                     CGTYTSAADGIVVAAPGTYHENNVDGSLGFTNDITQDGWDLGVPDQCGDYIMPGSPED
                     GFAIQIGTGPVNGNVQPYCSNPAVGLTDFPAFAGGNVTNTNTGAVRRSLWQGTNEDLG
                     LAVAQTTYYLSKKQAILSIVDICNGGDALTDVYYARNADPDNDQVTTGNFTTNNNSAK
                     QYATHGYSQVSASSATGSPCYMSYVTGDSRGKVSHGSFSMGSPNDMWNGVGGYVT"
     gene            complement(832..1206)
                     /locus_tag="PLB46_10070"
     CDS             complement(832..1206)
                     /locus_tag="PLB46_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76907.1"
                     /translation="MKRLILPVLLAAGMLLSSEGYGQQAIKVDQNFNIQLTDDLLQQK
                     FVQLDISGLTFKDEATATKYFNSIENNLVEFVLNYEAKTATMMLYPDRLGKFTYTLED
                     WNKYIATMSQRCSSNYNSFLNQ"
     gene            complement(1520..2860)
                     /locus_tag="PLB46_10075"
     CDS             complement(1520..2860)
                     /locus_tag="PLB46_10075"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HRF76908.1"
                     /translation="MKKSFYKYLTLSLIIVSVASCEDILNLEPAQSLSNETALDNDEG
                     VKQALIGAYDNMSQTSLLGGELMRNAELHGADNEILWVGTYTAPREIFNRDILNTNAD
                     VLDLWTDAYEVINTCNNVINAIDVVLAADQNRVRGEALALRAWAHFELTRMFGQQYEA
                     GIDNTQLAVPLILIPTLAFGDNVDVTRNTVEECYNQVIADLTEAESLLPEKNDVFINK
                     YTAAALLARVYLQKADYAAARDAADRVIASGDYELNATYAECFAQDDPTDEDIFSAEN
                     SELDNSNVMVTYFAINDFGGRADIEIEDSHLALYDDLDARKAMFFVDGDATFTSKYNN
                     EFGNLSIIRLAEMYLIRAECNSRLGTAVGAEPVDDYNMVHTRAGLPAEGAVALDDILL
                     ERRLELALEGFKVHDIKRLKGTISVMNYNDPKMIYPIPVSEIEINPLLEQNPGY"
     gene            complement(2897..>4985)
                     /locus_tag="PLB46_10080"
     CDS             complement(2897..>4985)
                     /locus_tag="PLB46_10080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HRF76909.1"
                     /translation="DWQSLAFQKAISSMLDLSISGGTEKMQYFTSVGYSYQDGIMLGN
                     HGDRFNILNNIDAQLTEKLKMSLNLNLSRSINNDIPDDNSFSTPLQMVAQAPITPPAD
                     TTGEYYNTPTTLYYNPLIEKDNVFFESTTFRTLSNIAAEYQLIPSLAIRGEFGGDITN
                     HYQERFYGSKSEVGLSVGGYATNYNATVQYLNTKLLLLFDKQIEKHSIGATGGMEYNR
                     YDDNYNYVEGQGFPNDKLQTLASAAEIVAGTSVDSYYRFLSYFARATYDYDTRYLLNI
                     TVRADGSSRFGEENRYGVFPSASVGWVMSNEAFLKGSKKVSLLKLRASYGLTGNACIG
                     NFDYKGLYGIGSYAGTSSIYPSTIANPALTWEKTAQLDIGFDFGFYNDRISGELDYYI
                     KNTTDLLLDVPVPATTGYALQTQNVGSLENKGYEIVINTQNFVGDFSWSTSFNFSVNK
                     NKILELAEGQELIDLGGSDFMNVIMVGQPLGVFYGAEYAGVDPDNGDGLFYVNETEGS
                     DETTTDFNLANYVVIGDPNPDFISGITNNFAYKNISLDVTLQSVYGNDVNLQGDYWMS
                     SNASQYDNQTIDQLDAWQNPGDETDIPENRLLFANGTEARSSRYLSDASYLRVKTVTL
                     SFTFPKRLTEKMNMSSLRLYTSAYNLFTFTNYTGWDPEVSTDSFTDNVYYGLDFYSAP
                     QPKTVVFGVSIAL"
BASE COUNT         1625 a          974 c          804 g         1582 t
ORIGIN      
        1 gtaacataac caccaacacc attccacata tcgtttggag atcccattga gaaactacca
       61 tgagaaactt taccacgaga gtcaccggtt acataactca tgtaacaagg agagcctgta
      121 gcactagatg cgcttacttg tgaataacca tgagtagcat actgttttgc agaattgttg
      181 ttagttgtaa agttgcctgt agttacctgg tcattatctg ggtcggcatt tcttgcataa
      241 taaacatcag tcaaagcatc gccaccatta caaatatcaa caatacttaa aatagcttgt
      301 tttttgctta gataatatgt tgtttgtgca acagccaaac ctaaatcttc atttgttcct
      361 tgccataaac ttcttctaac tgcaccagta tttgtattcg ttacgttacc accggcaaat
      421 gcaggaaaat cggttaatcc aactgcagga tttgaacaat aaggttgaac gttaccattc
      481 accggacctg ttcctatttg aatagcgaaa ccatcttcag gtgaaccagg catgatataa
      541 tctccgcact gatccggaac tcctaaatcc caaccatctt gtgtaatgtc atttgtaaat
      601 cctaaagaac catcaacatt gttttcatga taagttccag gtgcagcaac aacgatacca
      661 tcagcagctg atgtataagt tccgcaatca gaaatggcta cttctaccca tttccctttt
      721 aaataagaaa caccaccaac aatttgagcg tttgataatg tagctgaacc aataattagc
      781 attgctgcag ccgaaatttt tatataagtc tttttcataa gtttcagttt attattggtt
      841 taaaaatgaa ttgtaattag atgaacaacg ctgcgacata gtagcaatgt atttattcca
      901 atcttccaac gtgtaagtga atttacctaa acggtcagga tataacatca tggtagccgt
      961 ttttgcttca tagtttaaaa caaattcaac aaggttgttt tcaatggagt taaaatactt
     1021 ggttgcagtt gcttcatctt taaaagtcaa acctgaaata tcaagctgaa caaatttttg
     1081 ttgcaataaa tcatccgtca actgaatatt aaaattctgg tcaaccttaa ttgcttgttg
     1141 tccatatcct tctgaggata acaacattcc tgctgcgagg agcacgggta aaattaatct
     1201 tttcatctag gcaataattt aaaatgtgaa ctaatatatt tctatctaaa aataaccaaa
     1261 tactttaaga attctaagct ttgggtaggc agaaattaat aaagatgtca caatacttgt
     1321 cataattatc cataatttac gactagaaat aactaaataa aacattttta ttaagaaata
     1381 tccaattaaa ccatgtcaca aatattacaa attagtagta aaaaatagtc atttcaaaat
     1441 gtaccaatca ttcacaaaaa aaacgagccg gccaaagcca gctcgttaca atttgatttc
     1501 taacgtaata ttggttacct caatatcccg gattttgttc caggagtggg ttaatttcga
     1561 tttctgacac cggaattggg taaatcattt tagggtcatt gtaattcata acactgatag
     1621 tgcctttcaa acgtttgata tcgtgcactt taaatccttc gagcgcgagt tctaatctac
     1681 gttctaaaag aatatcatca agtgcaacag cgccctctgc aggtaaacct gctcttgtat
     1741 gcaccatatt ataatcatca acaggttcag cgccaactgc tgttcctaaa cgagaattac
     1801 attctgcgcg aattaagtac atctcagcta aacgaatgat actcaggtta ccgaattcgt
     1861 tattatattt tgaagtaaaa gtagcatctc catctacaaa aaacatggct ttacgagcat
     1921 ccaaatcatc atacaaagcg agatggctgt cttcaatttc gatatctgca cggccaccaa
     1981 aatcgttaat agcgaaatag gttaccatca cattactatt gtccaattct gaattttctg
     2041 cactgaaaat atcttcatct gtaggatcgt cctgagcgaa acattctgca taggtagcat
     2101 tcaattcata atcgccggaa gcaattactc tgtctgcagc atcgcgcgct gcagcataat
     2161 ctgctttttg taaatacaca cgagcgagta aagcagctgc agtatattta ttaatgaaca
     2221 catcattttt ttcaggtaat aacgattcag cttctgttaa atcagcaatt acctgattat
     2281 aacattcttc aacagtatta cgtgtaacat caacattatc gccaaatgct aaagttggaa
     2341 ttaaaattag cggaactgct aattgtgtat tgtcaattcc tgcttcatat tgttgtccaa
     2401 acatacgtgt caattcaaaa tgtgcccagg ctcttaaagc taacgcttct ccgcgaacac
     2461 gattttgatc agcagctaat acaacatcta ttgcattaat cacattgttg caagtattga
     2521 ttacttcata tgcatctgtc cataaatcca atacatcagc attagtattt aaaatatcgc
     2581 gattaaaaat ttcacgtgga gccgtataag ttcctaccca taaaatttca ttatcggcgc
     2641 cgtgtaattc agcgttgcgc attaattcac ctcccaataa agaagtttgg gacatgttat
     2701 catatgcacc tattaatgcc tgttttaccc cttcatcgtt gtcgagggca gtttcattac
     2761 ttaagctctg agcaggttca aggttcagta tatcttcaca agatgcaact gatactatta
     2821 ttaaactcag tgttaaatat ttatagaatg attttttcat gattattgtt ttttaaatta
     2881 caattcggtt atttaattaa agtgcaatac ttacaccaaa tacaactgtt ttaggttgtg
     2941 gagcactata aaaatctaaa ccataataca cattatctgt aaacgagtcg gttgaaactt
     3001 caggatccca accggtgtaa ttcgtaaatg taaataggtt atacgctgag gtatataatc
     3061 tcagtgatga catattcatt ttttctgtta atcgtttagg gaatgtgaaa cttaatgtaa
     3121 ctgttttaac acgtaagtaa gatgcgtcag ataaataacg gcttgaacgc gcttctgtac
     3181 catttgcaaa taacaaacga ttttcaggaa tgtcggtctc atcaccagga ttttgccaag
     3241 catctaactg gtcaattgtc tggttgtcgt attgacttgc gttagaactc atccaataat
     3301 caccttgcag gtttacatcg tttccgtata cactttgtaa tgtaacatcc aatgaaatgt
     3361 ttttataagc aaagttgttt gtaataccgg aaataaaatc aggattcggg tcaccaatta
     3421 ctacataatt agcaaggtta aaatcggttg tagtttcatc acttccttca gtttcattta
     3481 cataaaataa accatcacca ttatcaggat caacaccggc atattctgca ccataaaata
     3541 cacctagcgg ttggccaacc ataataacat tcataaagtc gctaccacct aaatcaatca
     3601 actcttgtcc ttcagctaat tccagaattt tatttttatt cactgaaaag ttaaagctgg
     3661 tactccagga gaaatcgcca acaaagtttt gagtattaat tacaatctca taacctttat
     3721 tttcgaggct acccacattt tgtgtttgta acgcataacc tgttgttgcc ggaaccggca
     3781 catctaacaa taaatcggta gtatttttaa tataataatc taattcacct gagatgcggt
     3841 cattataaaa accgaagtcg aaaccaatat ctaattgcgc tgttttttcc caagttaatg
     3901 ctgggttggc aattgttgat ggatatatgg atgaagtacc ggcataagaa ccaataccgt
     3961 aaagtccttt gtaatcgaaa ttaccaatac aggcatttcc tgttaaacca taacttgcac
     4021 gcaattttaa caaactcact tttttgctgc ctttcaaaaa tgcttcattc gacattaccc
     4081 aacccactga agcagaaggg aaaacaccgt aacgattttc ttcaccaaaa cgggatgaac
     4141 cgtcggcacg aactgtaata ttcaataaat aacgtgtatc ataatcataa gttgcacgag
     4201 caaaataaga taagaaacga tagtaagaat caactgatgt tcctgcaaca atttctgctg
     4261 cgctggctaa ggtttgtaat ttatcatttg ggaaaccttg tccttcaaca tagttgtagt
     4321 tatcatcata cctgttatat tccataccac ctgttgcacc aattgagtgt ttttctattt
     4381 gtttgtcgaa aagcaataac aacttggtat ttaaatactg cacagttgca ttgtaattag
     4441 tagcataacc tccaacagat aaacctactt cacttttgct gccataaaaa cgttcctgat
     4501 agtgattagt aatatcacca ccaaattcac cgcgtatggc taaagatggt attaattgat
     4561 attcagctgc gatatttgaa agtgtgcgga aagttgtgct ttcaaaaaac acattatctt
     4621 tttcaattaa aggattataa tataaagttg ttggtgtatt ataatattca ccggttgtat
     4681 cagctggcgg agtaattggc gcctgagcta ccatttgtaa aggtgttgaa aatgaattgt
     4741 cgtcaggaat atcgttatta atagaacgac ttaaattgag gttcaaactc attttcaatt
     4801 tttcggtaag ctgggcatca atattattta aaatattaaa acgatcgcca tggttgccta
     4861 acataatacc atcctgataa gaataaccaa cgctggtaaa atattgcatt ttttcagttc
     4921 ctcccgaaat agataaatcg agcatactag aaattgcttt ttggaaagca agtgattgcc
     4981 agtct
//
LOCUS       DAORKW010000244         4968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_744_length_4968_cov_4.958885, whole genome shotgun sequence.
ACCESSION   DAORKW010000244 DAORKW010000000
VERSION     DAORKW010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_744_length_4968_cov_4.958885"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="PLB46_10085"
     CDS             complement(<1..138)
                     /locus_tag="PLB46_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRF76910.1"
                     /translation="MKYYIIAGEASGDLHGANLVEAIKAQDAEAQFRGFGGDKMKAEG
                     VT"
     gene            complement(147..464)
                     /locus_tag="PLB46_10090"
     CDS             complement(147..464)
                     /locus_tag="PLB46_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76911.1"
                     /translation="MSKLQEDNVFVGMLVSVFFTVTMFYVFYQINESLSGEVYVNGKL
                     FYGVQERFIATLAVFFNIIPFVIYMRTRKDNCLRGVGIVTVLMAIFMMVYYYILHNNY
                     LIN"
     gene            complement(474..1316)
                     /gene="surE"
                     /locus_tag="PLB46_10095"
     CDS             complement(474..1316)
                     /gene="surE"
                     /locus_tag="PLB46_10095"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRF76912.1"
                     /translation="MANNNQPTILVTNDDGILAPGIHALVNAVKDLGKVVVVAPNKPQ
                     SGMGHAITLNEPLRLKKSNVFGDIEAYECSGTPVDCVKLAKDKILHKKPELCVSGINH
                     GSNSSINIIYSGTMSAAMEAGIEGIPAVGFSLLNYAHDADFTGAQHFAKIIAQQILTA
                     GLPQGTLLNVNIPDLPVDRLNGIKICRQAVAKWEEEYTERNDPAGRPYYWLTGKFVNY
                     DKGEDTDEWALSNGYVSVVPVQYDLTAHHAIAYFNKNWNVNAISNVHHEVHIDTSKSF
                     NDLG"
     gene            complement(1409..2905)
                     /locus_tag="PLB46_10100"
     CDS             complement(1409..2905)
                     /locus_tag="PLB46_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76913.1"
                     /translation="MNRCKIWMLIFGLLTVQAVQAQDDFIQRADKLYKNQIYDQAADM
                     YLTYLQQTYNYEINLKLAECYRLMHSTVDAEYWYGVIVTQNAADPDILRRYADLLKTN
                     GKYRSAKEYYLKYAAYEEDGYYYAASCDWAITNRSKAPAYFIDTLGFNTTDSELTPTF
                     YKRGLIYARSTGNKINPTTGVSFYDLYYTELRNDSLWIISPMQNVNSEVHEAAPYYDG
                     NDQKLYFTRNNHYRGRTIKSSDGEVKLELYFSTSVDNKFTGPRPLSVNSKTYSVGQPT
                     VSPDGKVLIFASDKPGGYGGTDLYFCTKKGNDWSNAKNMGPAINSKGDELYPSMSPEG
                     TLYFASNYHPGFGGFDIFKSVRVNQYWSNPENLGMPVNSSQDDYALIMRNGVGYFTSN
                     RAGGQGSDDIYQVTQMATLSKIYVYDTDIKPIYKAKVTFIESPNMQVICETDASGLGD
                     ISTLSGNTMAIKISKEGFLDKIVYDLSSLRSSNGIIPVELQPLLGTNE"
     gene            complement(2917..3882)
                     /locus_tag="PLB46_10105"
     CDS             complement(2917..3882)
                     /locus_tag="PLB46_10105"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF76914.1"
                     /translation="MKKLTTTLLLVAGGLLSNVYGQLQPLYDQYHFNQLVFNPGYAGS
                     KGMLETNFFLHRHAVNFEGAPGSESFTVHTPLANDKIGIGVKFFHDKLGVTNTNFLGI
                     DYAYRMHINSNLTASIGIEASIANYTVNYSDLDAYADGDPTFTGASDTYWAPNAGVGL
                     YLHSDSYYLGVSTVNLLGASDDDTQTESEPYDDHFDQVRTIYATLGTLITVNENFAIK
                     PDAMVKMAESLPSQLDVNMNFIFNNMFLVGGGYRTNGSFGSYSVAAQYLFSTDNNITA
                     HEAGFGYSYSAALTDDAVFLSPSHEVFLVYRFNKHNNKIKNPRFF"
     gene            complement(3879..>4968)
                     /locus_tag="PLB46_10110"
     CDS             complement(3879..>4968)
                     /locus_tag="PLB46_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRF76915.1"
                     /translation="VMSNDLNTTGETNVYLTILTPPANGTVVVDEINDLLIYTPNPDF
                     AGTDNFVYNGCADTDPDICGSATVIVTVNQIPDKPIANDDLYNTYINTPFNAEHLLND
                     IDIDDEGLEYFIIDDADNCATTAIGDEILYVEPISDFLGTDEVTYAVCKIGSLVYCDT
                     ATITINVLSTNFNAPAAVNDTVEIYLDFSGVIYPLLNDFDGDGDNLTITEILTDGITG
                     IASFSADSVSYGALELGEDVFSYVVCDDNLPSLCDTAQIVVNVVTPPVGELVVHVPNS
                     FSPNGDGINDLLEVEGLPELTRFNLKIYDRWSSLVYENVDQSKTWDGKSNVEFVSSSG
                     DVPEGTYYYVLRINGFPDPMAGFIVVKR"
BASE COUNT         1586 a         1030 c          826 g         1526 t
ORIGIN      
        1 agtcacccct tccgctttca ttttatcgcc accaaatcct cgaaattgtg cttcagcatc
       61 ttgcgcctta atagcctcta caagatttgc accatgtaaa tcgcctgaag cctctcctgc
      121 tatgatgtaa tatttcacaa cagcggttaa ttaattaaat aattattatg cagaatataa
      181 taataaacca tcataaatat ggccattaaa acggtaacaa taccaacgcc gcgcaaacaa
      241 ttatcttttc gtgtgcgcat ataaatgaca aacggaataa tattaaaaaa tactgccagt
      301 gtagcaataa atcgttcctg cacgccataa aataatttgc cgtttacata tacctctccg
      361 cttaaacttt cattaatctg ataaaacaca taaaacatgg taacggtaaa aaaaacggat
      421 accagcatgc ctacaaaaac attatcttct tgaagtttac tcatagtgta aaattatccc
      481 aggtcgttaa atgattttga tgtatcaata tgcacctcgt gatgcacatt cgaaatagca
      541 ttaacgttcc aatttttatt aaagtatgca atggcgtgat gcgcagttaa atcgtactgc
      601 accggaacaa cagaaacata tccattactc aacgcccatt catcggtgtc ttcaccttta
      661 tcgtagttga caaacttacc ggtaagccag taatatggtc gcccagcggg gtcgtttctt
      721 tcggtgtatt cttcttccca tttcgcgaca gcctgtctgc aaattttgat accatttaat
      781 cggtccaccg gtaaatcggg aatatttaca tttaacaaag tgccttgcgg taatcctgca
      841 gttaaaattt gttgcgcaat aatttttgca aaatgttgtg cgcctgtaaa atcggcatca
      901 tgagcgtagt tgagcaacga aaatccgaca gccggaatac cttcaatacc cgcttccata
      961 gccgcactca tggtacctga ataaataata ttgatagaag aattactacc gtgattaatt
     1021 cccgaaacac ataattccgg ttttttgtgt aaaattttat ctttcgccag cttcacacaa
     1081 tcaacaggag tgcctgaaca ttcgtaggct tcaatatcgc caaatacatt cgattttttt
     1141 aagcgcaaag gttcatttaa tgtgatggca tgacccattc cgctttgtgg tttattggga
     1201 gcaaccacca ccactttgcc caaatctttt actgcattta ccaaagcgtg aatacccggt
     1261 gcaagtatac catcgtcgtt ggttactaaa atggtaggtt gattattgtt agccataaca
     1321 aacaaaatta attggtatta ctaccgtgta ataattaaac atgtaaaatg attattaata
     1381 tgtgtaatca atttacatcc tgaagccatt attcgttggt acccagcaaa ggctgcaatt
     1441 caaccggaat aattccattg cttgatctaa ggctactgag gtcgtaaaca attttatcga
     1501 ggaaaccttc tttcgaaatt ttaattgcca tggtattacc ggaaagcgtg ctgatatcac
     1561 ctaatcccga agcatctgtt tcgcaaatta cctgcatgtt cggactttca ataaaggtta
     1621 ctttggcttt atatatcggt ttaatatcgg tatcgtacac atatatttta ctcaatgttg
     1681 ccatttgagt aacctgataa atatcgtcgc ttccctgacc acctgcgcgg ttagaagtaa
     1741 aataacccac accattgcgc ataattaatg cataatcatc ttgtgatgaa tttaccggca
     1801 tacccaaatt ttcagggttg ctccaatatt gattaacacg caccgattta aaaatatcaa
     1861 agcctccaaa tcccggatga taattgctgg caaaatataa agtgccttcc ggactcatag
     1921 aaggatataa ttcatcgcct ttgctattaa ttgcaggtcc catgtttttt gcattcgacc
     1981 agtcgttgcc ttttttggtg cagaaatata aatcggttcc accatatcct cccggcttat
     2041 ccgatgcaaa tatgagtact tttccatccg gagaaactgt cggctgacca acagaatatg
     2101 tttttgaatt tacagacaat ggtcgtggtc ccgtgaattt attatccact gaggtcgaga
     2161 aatataattc caattttact tcaccatcgc tggatttaat tgttcttccg cggtagtgat
     2221 tatttctggt gaaatagagt ttttggtcat tgccatcata ataaggagct gcctcatgta
     2281 cttcggaatt cacattctgc attggcgaaa taatccaaag tgaatcgtta cgtaattctg
     2341 tataatataa atcgtaaaaa gaaacacctg tggttggatt aattttattg cccgtgctgc
     2401 gcgcataaat taatccccgt ttgtaaaatg ttggtgttaa ttcagaatct gttgtattaa
     2461 atccaagtgt atcaataaaa taagcaggcg cttttgatct gttggttatt gcccagtcgc
     2521 aactggcagc ataataataa ccatcctctt cataagcggc atatttcaaa taatattctt
     2581 tcgctgagcg atatttacca ttcgttttta ataaatcggc ataacgacgt aaaatatccg
     2641 ggtcggccgc attttgggtg acaattacac cataccaata ttccgcatca acagtactat
     2701 gcatcaaacg ataacattcc gccaacttga ggtttatttc atagttgtag gtttgctgca
     2761 aataagtaag atacatatcg gctgcctggt cataaatttg atttttatat aacttgtctg
     2821 cgcgctgaat aaaatcatcc tgggcttgaa ctgcctgaac ggtcagtaag ccaaaaatca
     2881 acatccagat tttacaacga ttcatcatat caggtttcag aaaaatcttg ggtttttaat
     2941 tttgttatta tgcttattaa atctgtatac taaaaatact tcgtgagaag gacttaaaaa
     3001 tacagcatca tcggttaaag cagcgctata ggagtaacca aaacctgctt catgtgcggt
     3061 gatattatta tcggttgaaa ataaatattg agctgcaacc gaataactgc caaaacttcc
     3121 attcgtgcga tatcctcctc caactaaaaa catattgtta aatatgaaat tcatatttac
     3181 atctaattgt gaaggcaaac tttccgccat tttaaccata gcatccggtt taattgcaaa
     3241 attttcatta acagttatca gcgttcctaa agtggcataa atagttctta cctgatcaaa
     3301 atgatcatca taaggttcac tttccgtttg tgtatcatcg tctgaagcgc ctaataaatt
     3361 cactgttgaa acacctaaat aatagctatc gctatgcaga tataaaccca ctcctgcatt
     3421 tggtgcccaa tatgtatcac ttgcgccggt gaatgtagga tcaccatctg cataagcatc
     3481 taaatcgcta taattaactg tgtaattagc aatagatgct tcaataccaa ttgaagccgt
     3541 taaattcgaa ttaatatgca tacgataagc ataatcaata cctaaaaaat tcgtattggt
     3601 tacacccagt ttatcgtgaa aaaatttcac tccaatacca attttatcat ttgccaaagg
     3661 tgtatgcacg gtaaatgatt cagaacccgg agcaccttca aaatttaccg catggcgatg
     3721 caggaaaaaa ttggtttcca acatgccttt gcttcctgca tatccgggat taaataccaa
     3781 ttgattaaaa tggtattggt cgtacaaagg ttgcaattgt ccgtacacat tcgacaacaa
     3841 cccgccggca acaagtaata gtgtggtagt taatttcttc atcgtttaac aactataaat
     3901 cctgccattg ggtcaggaaa tccattaatt cttaaaacat aataataagt accttcagga
     3961 acatcacccg aactggaaac aaattcaaca ttcgatttgc catcccaggt tttgctctgg
     4021 tcaacatttt cgtaaaccag actgctccaa cgatcatata tttttaaatt aaatcgtgtt
     4081 aattcaggta aaccttctac ttctaataaa tcgttaatgc catcaccatt tggagaaaac
     4141 gaatttggca catgtaccac taattcacca acaggaggtg taacaacatt cacaacaatt
     4201 tgtgcagtat cacacagtga aggtaaatta tcgtcgcaca caacataact aaacacatct
     4261 tcacctaatt cgagtgctcc gtaactaact gaatcggcac taaatgaggc aataccggta
     4321 ataccatcgg ttaatatttc cgtaatagtt aaattatcac catcgccatc aaaatcgttg
     4381 agcaaaggat aaataacgcc ggaaaaatct aaataaattt caaccgtatc gttaaccgca
     4441 gcaggtgcat taaagttggt agataaaaca ttaatagtaa ttgttgcagt atcgcaatac
     4501 actaaagaac caattttaca aacggcataa gttacttcat cagttcctaa aaaatcacta
     4561 ataggttcca cgtataaaat ttcatcacca attgcagtgg ttgcacaatt atcggcatcg
     4621 tcaatgataa aatattctaa accctcatca tcaatatcaa tatcatttaa taaatgttcc
     4681 gcattaaatg gggtattaat gtaagtgttg tataaatcgt cgttcgcaat tggtttatcc
     4741 ggaatttgat ttaccgtaac aatcactgtt gccgaaccac aaatatccgg gtcggtatcg
     4801 gcgcaaccat tgtaaacaaa attatcggtg ccggcgaagt caggatttgg tgtataaatc
     4861 aacaaatcat ttatttcatc cactaccaca gttccgtttg ccggtggtgt aagaatggtg
     4921 agataaacat tcgtttcacc ggtcgtattt aagtcgttcg acattacg
//
LOCUS       DAORKW010000245         4966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_745_length_4966_cov_4.407249, whole genome shotgun sequence.
ACCESSION   DAORKW010000245 DAORKW010000000
VERSION     DAORKW010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_745_length_4966_cov_4.407249"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLB46_10115"
     CDS             complement(<1..707)
                     /locus_tag="PLB46_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF76916.1"
                     /translation="MMKHFLLLLFTGILTVSGFAQSTFPVNGVADNRERVYAFVHATI
                     VVDPTTTIADATLLIQSGKILGAGTNVTIPADAIVVESKGKFIYPSFIDLYSGYGIPA
                     KQNPQQGRGPQMLNNNTNATNWNQAVTPEFEAFSFFSVDTASNKKWRNAGFGTTLTHR
                     KDGILRGTGALVLLGDESSGKMVMKEKVSSHYSFLKGSSTQDYPSSQMGSIALIRQTL
                     YDADWYGKGGDKIEKNVS"
     gene            878..3223
                     /locus_tag="PLB46_10120"
     CDS             878..3223
                     /locus_tag="PLB46_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF76917.1"
                     /translation="MYWRKLVILVTFTLVWGNIFAFHIIGGEIIYTRTTGNNFTITLK
                     IYRDCADPEAAPYDDPLQIYVYDVSGILVQTLDIYFPGSELIDPAIVSPCMNVYPDLC
                     VQEAIYTTTVNLPPKAGGYDLVYQRCCRNSTIINIVDPVATGATYTTHIPDPGEINNS
                     SPRFNSLPPISLCAGFPFEFDHAATDPDGDELVYEFFTPYSGATPDVPDPDPAPAPPF
                     NEVFFIDPFNEDYPIASDPAFTINPVTGYMEGTPTMLGQFVVGVAVKEYRDGVLIGTH
                     YRDFQFNVTDCEPTAVANAPNVIKDCTDYSVTFENYSYGSDEYYWDFGIDGITSDTST
                     AENPTYVFPDTGVYEVMLVVLPGSECSDTDYVQVWIYPGFDANISFDNSCANTDIQFT
                     DLSTTVYGTITNWDWNYGNGSGSTEQNPLYSYNAQGEYTIYFTVTNSYGCVAELYDTI
                     TIYPSPYSNFVFDNACIGQTGIIEDASFIFIGNEIVNTEWYLPDGSYVNDDAVEYYFD
                     TAGYYPIILISTSNLGCTDTLYDTIIVPEPVVASELTDVTICEGDSVQYIASGGIYYE
                     WLPNVEVSGNNNDSTFLSPTSTTTYAVIVSDECTSDTAFVTINVLPAPDIIAGPDTIV
                     YSNHPVQMYAEGGISYVWEPAFGLDNAFIENPIASPDKTTQYIITGSDESGCKAVDTA
                     LVYIIPSCFYYTTVNAFSPNGDGINDRFRFITFGDEEILLMEIYNRWGEKIYATTSLE
                     NGWDGTDGNGVEQEVGAYVYRLITICDGIEQALSGSVTLLR"
     gene            3264..>4966
                     /locus_tag="PLB46_10125"
     CDS             3264..>4966
                     /locus_tag="PLB46_10125"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF76918.1"
                     /translation="MKNLRTLLLVLTLSLCFNQAKATHIIGGEITYVCLGDSTYQIKL
                     ILYRDCTSATPFDTPAYMSVFTPEGEFVFNFTIPAPVISDVPAESENPCLDAPDDICV
                     EQAIYTKTIFLPPSETGYVVVYQRCCRNAGIVNIILPDNTGSTCTQDVPPTSVVECNR
                     SPVFNDFPPTVICLNDPIEFDHSATDADGDSLAYFFYWPYEGATAVDAAPVIASPPPY
                     AGVGWAAGFDELYPITADPAFSINSETGLLTGTATAEGRYVVGVVCKEYRDGVWLGDH
                     NRDFQFNVVACAPLVDAEIEIGDAIFTEVDSTSVFLNCDDYTIYFENNSIGGDSYFWD
                     FGDGLTSTEVNPVHTYTDTGTYYVMLIVNPGFVCADTALGQIGVYNGLAANYSFITGC
                     AGTGVEFNDLSVSTNAGEIVDWDWVFDDGSTSDLQNPEHVYAVGGTYTVSLHIVSTSG
                     CESDYVLEVEVAPGPTTEFEVDDVCQNFAAEFDNQSTIVDGTISGYLWDFGDGQTSTE
                     ENPSHFYDEHGTFDVTLITYAANGCTDTITYDVLIGELPLADAGDDDSVLYLETFTLD
                     GS"
BASE COUNT         1406 a          957 c          993 g         1610 t
ORIGIN      
        1 gaaacatttt tttcaatttt atcacctcct ttaccatacc agtcggcatc atacaaagtt
       61 tggcgaatta atgcaatact acccatttgt gatgacggat aatcttgtgt tgaactgcct
      121 tttaaaaagg aataatgact actcactttt tctttcatca ccattttacc actagattca
      181 tcgcctaata aaactaatgc tcctgttcca cgtaaaatac catctttacg atgtgttaaa
      241 gttgttccaa aaccggcatt ccgccatttt ttgttcgagg ctgtatctac actaaaaaaa
      301 gaaaaagctt caaattccgg tgttacagcc tgattccaat tagtggcatt ggtgttatta
      361 tttaacattt gtggtccccg gccctgttgc gggttttgtt ttgcaggtat accgtatcct
      421 gagtataaat cgataaatga agggtaaata aatttccctt tcgattctac cacaattgca
      481 tcagccggaa tagtaacatt tgttcccgct cctaaaattt tcccggattg aatgagcaag
      541 gtggcatcag caatcgtagt agttgggtca acaactatag tcgcatgcac aaaagcatat
      601 accctttccc gattgtcggc aacgccgttt accggaaatg ttgattgcgc aaaccccgaa
      661 acagtaagta ttccggtaaa aagtaatagc aaaaaatgtt tcatcattgt taaaaattag
      721 aatcttttag tcacttattt ttgattgaaa tgtgcactaa gataaaaatt gcggtgataa
      781 acaactgtta aaccttttta gtaaaaccat cgtctataac cttaaatgac attcctatga
      841 atgattttaa caaattcctg aggtaattta gcagtttatg tactggagaa aactggttat
      901 actcgtcact ttcacattgg tatggggtaa tatatttgcc tttcatatta ttgggggtga
      961 aattatatat accaggacaa ccggtaacaa ttttactata acattaaaaa tttatcgcga
     1021 ttgtgccgac ccggaagccg cgccatacga cgatccactg caaatttatg tgtacgatgt
     1081 ttcgggtatt cttgtccaaa ccctcgacat ttatttcccg ggatcggagt taattgaccc
     1141 ggctatagta agtccgtgta tgaatgttta tcccgattta tgcgttcagg aagccattta
     1201 tactacaacc gtaaatctac ctcccaaagc agggggttac gatttggtat accaacgttg
     1261 ctgtcgcaat tcaaccatca ttaatattgt tgaccctgtt gcaacaggtg ctacttatac
     1321 cacgcatatt cccgatccgg gtgaaataaa taattccagt ccgagattta attcattacc
     1381 tcccatttct ttatgtgctg gatttccctt tgaatttgac cacgctgcta ccgacccaga
     1441 cggggatgaa ttggtttacg aattttttac accttattcc ggcgctacac cagatgtgcc
     1501 cgatcccgac cctgcacctg ctcctccatt taatgaagta ttttttattg acccatttaa
     1561 tgaggattat ccaatagcat ccgaccctgc atttaccatc aatccggtta caggctatat
     1621 ggaaggaaca cccacaatgt taggacagtt tgttgttggc gtagccgtta aggagtatcg
     1681 tgacggtgta ctcattggta cccattaccg tgattttcag tttaatgtaa cagactgtga
     1741 acccaccgca gtggcaaatg caccaaatgt tataaaagat tgcaccgatt attcagtcac
     1801 tttcgaaaat tatagttacg gttcagatga atattattgg gattttggaa ttgatggtat
     1861 aacgagtgac accagcactg cagaaaaccc cacatatgtg tttccggata caggcgttta
     1921 cgaagtaatg cttgtagttt tgccaggctc tgagtgttct gatactgatt atgtgcaagt
     1981 ttggatatat ccgggtttcg acgcaaatat cagtttcgat aatagctgtg caaatacaga
     2041 tatacaattt actgatttat ctaccactgt ttatggtacc ataaccaatt gggattggaa
     2101 ttatggcaat ggatcaggca gcactgaaca aaatccattg tatagttata atgcgcaagg
     2161 cgagtataca atttatttta cggtgaccaa ttcttatggt tgtgtagcgg aattatatga
     2221 tactataaca atttatccct caccttatag caattttgtt tttgataatg catgtatcgg
     2281 ccaaaccgga attattgaag atgcttcatt tatttttatt gggaatgaaa ttgtaaatac
     2341 agaatggtat ttgccggatg gcagttatgt gaatgatgat gcggttgaat attattttga
     2401 tactgccggt tattacccta tcattttgat ttcgacaagt aatttagggt gtaccgatac
     2461 actttatgat acgattattg ttcctgaacc tgttgtggca agtgagttaa ccgatgttac
     2521 tatttgcgaa ggcgacagtg ttcaatatat tgcatcaggt ggtatttatt atgagtggtt
     2581 gcccaatgtt gaagtttcag gaaataataa cgattccact tttttatcac ctacaagcac
     2641 taccacttat gccgtaatag tttctgatga atgtactagc gacacggcat ttgttacaat
     2701 aaatgtttta cccgctccag atattattgc cggacccgat accatagtgt atagcaatca
     2761 tccggtacaa atgtatgccg agggtggcat tagttatgtt tgggagccag cttttggttt
     2821 agataatgca tttatagaaa acccgatagc ttcaccagat aaaaccacac aatatattat
     2881 aaccgggtcc gacgaatcgg gatgcaaagc cgtagataca gctttagttt atataattcc
     2941 aagctgcttt tattacacta cagtaaatgc cttttcacca aatggagatg gaataaacga
     3001 cagatttagg tttatcactt ttggagatga agaaattttg ctcatggaga tttataatcg
     3061 ctggggtgaa aagatttacg ccactaccag ccttgaaaat ggatgggatg gaacggatgg
     3121 taatggggtt gaacaagaag taggagccta tgtttaccgg ttaataacca tttgcgatgg
     3181 cattgagcag gccttaagtg gcagtgtaac attgttgagg taatttattt tacctatttt
     3241 taacttctta accaactcaa ataatgaaaa accttaggac tctcctcctt gtgctcacac
     3301 tatccttatg ttttaatcag gcaaaagcca cccatattat tggtggcgaa ataacctatg
     3361 tttgtttggg tgatagcacc tatcaaataa aactcatatt gtatcgtgat tgcaccagtg
     3421 ccactccgtt tgacactccc gcttatatgt cagtgtttac tcctgaaggc gaatttgtat
     3481 tcaactttac cattccagct ccggtaatct ctgatgtgcc cgcggaatca gagaatccat
     3541 gtttggacgc acctgatgat atttgtgtgg aacaagcaat ttatactaaa actatcttcc
     3601 taccaccttc tgaaacaggt tatgtggttg tttatcagcg gtgttgcaga aatgccggta
     3661 ttgtaaacat tattttaccg gataatacag gttcaacttg tacacaggat gtgccaccaa
     3721 cttctgttgt tgagtgcaat agatcaccgg tatttaatga ttttccacct actgttatat
     3781 gtttaaacga ccctatagaa tttgaccatt cggcgaccga tgctgatggt gattcgctcg
     3841 cttatttttt ttattggcca tatgaaggtg ctactgcagt tgatgcagca ccagtaattg
     3901 cttcacctcc accatatgca ggcgtaggtt gggctgcagg ttttgatgaa ttatacccaa
     3961 taacagcaga ccctgcattt tcaatcaact ctgaaacagg tttactcacc ggaacagcta
     4021 ctgccgaagg caggtatgtt gttggtgttg tttgcaaaga atatcgtgat ggcgtttggc
     4081 ttggtgatca caatcgtgat tttcaattta atgttgtggc atgcgctcct ttagttgatg
     4141 ctgaaattga aatcggcgat gctattttta ctgaagtaga ctccacctca gttttcctaa
     4201 attgtgatga ttataccatc tatttcgaaa ataatagcat tggcggtgat tcctattttt
     4261 gggattttgg agatggttta acgtcaaccg aagttaatcc tgttcacacc tatactgata
     4321 ccggaaccta ttatgtaatg ttaattgtaa atccgggatt tgtttgcgca gatactgctt
     4381 taggccaaat aggtgtttat aacggattag ccgctaatta ttcttttatt acaggctgtg
     4441 ccggaacagg tgttgaattt aatgatttat ctgtttcaac aaatgctggt gaaatagttg
     4501 attgggattg ggtttttgac gacggctcaa catctgattt gcaaaatcca gaacatgttt
     4561 atgcggttgg tggtacctat acagtctcgt tacatatcgt ttcaacctcg ggttgtgaat
     4621 cagattacgt attagaagtt gaggttgctc ccggccctac tactgaattt gaagtggatg
     4681 atgtttgcca aaattttgct gcagaattcg acaaccaatc aacgattgta gacggcacta
     4741 ttagcggata tctatgggat tttggcgatg gacaaacatc aaccgaagaa aacccatctc
     4801 atttttatga tgaacatgga acattcgatg tcacgctaat tacatatgct gctaacggtt
     4861 gtactgatac gattacctac gacgtgctta taggtgaatt gcctttggct gatgcaggtg
     4921 acgatgactc tgttttatat ttggaaacat ttacacttga tggttc
//
LOCUS       DAORKW010000246         4962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_747_length_4962_cov_4.754636, whole genome shotgun sequence.
ACCESSION   DAORKW010000246 DAORKW010000000
VERSION     DAORKW010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_747_length_4962_cov_4.754636"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PLB46_10130"
     CDS             complement(<1..957)
                     /locus_tag="PLB46_10130"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HRF76919.1"
                     /translation="MGLNIWLILGGVGIFLFGIMLLEQALQKLMSRRFKLFLRKQTGS
                     TFRAIFSGTLITAALQSSSVVNFMVLAFVSSGVITLKNALAVIMGTNLGTTLNSWIVA
                     TIGFKLEIEAFAYPVAGLAGMALFIFKQKTNLEPLFKFLMGFSFLFIGLGLMKELAES
                     EDLQAVFSRFQDSGAWVFLILGFIATTITQSSAATVAITLSFLNQQLLGFESAMAVVI
                     GSEVGTSVKLLLGSLDGVAVKQRVSVGNFLYNVVTTIVAFALLQPTAIFIQQILGISN
                     PLTGLATFQTLMNFGSIILFLPFLKMFSRWLEKTFNKDDRKLT"
     gene            1067..4531
                     /locus_tag="PLB46_10135"
     CDS             1067..4531
                     /locus_tag="PLB46_10135"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HRF76920.1"
                     /translation="MQLFIFTPHNSKGMNSFSRINNITGWLVFAVALIVYTMTMEATA
                     SFWDCGEFIAATYKLQVVHPPGAPIFLMVGRIFTLFASGPEQVPLMTNFFSALSTAFG
                     ALFVFWIITRMMRKSLAGTNEPSGSQLATIMFGGIIGATCFLFMDSIWFSAVESEVYA
                     LATFFFILIFWSIVKWEAHADDPNSDRWILFMALMMGLSIGVHLLALLVIPAIALIYY
                     FRNYTYSRKGLIYTLLIGTALVAFVLYGLLDKFIAIAAAFDRTFVNDIGLPIGSGIIV
                     FSLIVVGGIVYGIRHAIQRNKRILYITMMSFTMLIIGLTSYAMVLIRAKAEPVINMNG
                     INDVHSFLSYLKREQYGSRALFYGPYWTAQPIQYEKGKAKFGVVPGKDGYQVISHEFE
                     PVYDIPAEYLNSPNVSENARLIQERNKQVLFPRMGSLEDRHAGLYYDFANVDPAAEQT
                     YVPTYGENLKFLFNYQLGHMFWRYFMWNFSGRQNDTQGNFYDGYKDGNWVTGIEAIDK
                     TKNEQLEEVPPSLKNVLTHNNYYMLPFILGIMGMVYQLRKDKRGFAVVLTLFLFMGIM
                     NIINMNQPPTEPRERDYAQVGAFFAFAIWIGFGIGAIIDLAKNFKSKTIQEYVLYSAL
                     ILLSMFFLGLTMYSFTNFLMISAYLIIVMLLMGGIAYVINNAMKKEMALAVVAFLLCI
                     PAPFLMASQGWDDHNRSDRTFARDIARNYLESCPPNAILFTQGDNDTYPLWYAQEVEN
                     IRPDVKIINLSLLGVDWYINQLRHATNESAPIALTLSADKILGDKRNQVVDNDKSKFK
                     NQTMELKDALEFIGQDDPKFQVYSEGLEDYFSYVPTKKFKVTIDAEKMKANNIVPEAL
                     VEDMKKELNFELKRGTLLKNDLMTLDIVASNINNRPICFAISVTADSHLGMEKYLMQR
                     NMVYQLMPVEMENNKSQMDGYRKQMNTDIQYDLLVNQKDKFTYGGVERGGEMYIDPSG
                     MGSVLTTKYLNYFELAKNLDQERQYLEAQARQMMTDTVNKEYNEVGQQLMQESADKKK
                     KTIEVLDLMQTLFPDSSVPYDFNMVNVAQVYQRLGENEKAMAIVTTMKERVMQELEYY
                     YKMTLKSSLTASMFEDDKLRSETWVYYTVNMARSAGDKDFAEALDKEWETLRMKYGIL
                     APNERGRK"
     gene            4512..>4962
                     /locus_tag="PLB46_10140"
     CDS             4512..>4962
                     /locus_tag="PLB46_10140"
                     /inference="COORDINATES: protein motif:HMM:NF019644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HRF76921.1"
                     /translation="MREAENKRQRKKFDNLVFGRQPVAELLNSDKTVDKILLQNSAQG
                     DIVPLIKQKATEKAIPLQYVPIEKLNALTGGNHQGVIAFTAAISFQKIDDILPFIIEH
                     GETPLLLILDGITDVRNFGAIARTAWASGVHAIIIPAHSAAPVNSDAI"
BASE COUNT         1598 a          915 c          949 g         1500 t
ORIGIN      
        1 ggttagtttc cggtcgtctt tattaaatgt tttttctaac caccggctga acatcttcaa
       61 aaacggtaaa aacaaaataa tactgccaaa attcatcaag gtttgaaaag tggctaaacc
      121 agtcagcgga ttacttatgc ctaaaatttg ttgtataaaa atggctgttg gttgcaaaag
      181 tgcaaaagca acaatggtag ttaccacatt atataaaaag ttacccaccg acactctttg
      241 tttaaccgca accccatcga gcgaacctaa cagcaacttt accgatgtgc caacctctga
      301 acctattaca acagccattg cactttcaaa acccaataat tgctgattca ggaaactcaa
      361 agtaatggca accgtagctg cgctgctctg cgtgatggta gtggcgatga accctaaaat
      421 caggaaaacc caggctccgc tgtcctgaaa acgactaaat actgcctgca aatcctccga
      481 ttcagccaat tccttcatta aacctaggcc aatgaataaa aagctaaagc ccatcaaaaa
      541 cttaaaaagt ggctctaaat tggtcttttg cttaaaaatg aacagtgcca ttcccgccaa
      601 accggcaaca ggatatgcaa atgcctcaat ttctaatttg aagcctattg tagccactat
      661 ccagctgttt agggtagttc cgaggttggt ccccataatt accgccaggg catttttaag
      721 ggttattacc ccggaactga caaatgccag caccataaaa ttgaccactg agctgctctg
      781 taacgcagca gtgataaggg ttccgctaaa aatcgcccga aaggtactgc ccgtttgttt
      841 cctcaaaaac aacttaaacc gcctgctcat cagtttttgt aaggcctgct cgagcagcat
      901 aatgccaaaa aggaaaatcc caacacctcc taaaatcaac caaatgttta gtcccataag
      961 tatttgaaac agctaaccta cttaatattt agttaatata aaattagtaa aaagcccctg
     1021 ttaacatatt tcacaaataa taacaccaca agtgaaatac cttcatgtgc aattatttat
     1081 ctttacgcct cataactcga aaggaatgaa tagtttcagc aggattaaca acattacagg
     1141 atggctcgta tttgcggtag cccttatcgt ttataccatg accatggaag caaccgccag
     1201 tttttgggat tgcggtgagt ttattgcagc aacctataaa ttgcaggtag ttcacccacc
     1261 aggggctcct atctttttaa tggtaggccg catatttaca ttatttgctt caggccctga
     1321 acaagtgcct ttaatgacaa acttcttctc agcattatct actgcttttg gagcgctttt
     1381 cgtgttttgg attattaccc gcatgatgcg caaatcactt gccggaacaa atgaaccaag
     1441 cggtagccaa ctcgctacta tcatgtttgg tggtattatt ggtgctactt gtttcttatt
     1501 tatggacagc atttggtttt cggctgttga atcagaagta tatgcattag caactttctt
     1561 tttcatatta attttctggt ctatcgttaa atgggaagcg catgccgatg acccgaactc
     1621 tgatcgctgg attttattta tggcattgat gatgggttta tctattggcg ttcacttact
     1681 cgccttattg gtaattccgg ccattgctct catctattat ttccgtaatt acacgtattc
     1741 tcgcaaagga ttaatttata cgttattgat tggaacggct ttagttgcat ttgtattata
     1801 tggcttgctc gataaattta tcgctattgc agctgctttc gacagaacat ttgtaaatga
     1861 tattggctta cctatcggca gcggtattat agtattttca ttaattgttg ttggaggaat
     1921 tgtttatggt attcgtcatg ctattcaacg caacaaacgt attttataca tcaccatgat
     1981 gtcgtttacc atgttaatta ttggtttaac atcttatgct atggtgttaa ttcgtgccaa
     2041 agcagaaccg gtaattaata tgaacggtat taacgatgtg cactcgtttt tatcttattt
     2101 aaaacgtgag caatatggca gtcgcgcatt attttacgga ccatactgga cagcccagcc
     2161 aattcaatat gaaaaaggca aagcaaaatt tggtgttgtt ccgggtaaag atggttatca
     2221 ggtaatttct catgaatttg aacctgtata tgatatccca gctgaatatt taaactcacc
     2281 aaatgtttct gaaaatgcaa gattaattca ggaacgtaat aaacaagttt tattcccgcg
     2341 tatgggttca ttggaagatc gacatgcagg tttatattat gacttcgcca atgttgaccc
     2401 ggctgcggaa caaacttatg tgccaaccta tggtgaaaat ttaaaatttt tattcaatta
     2461 tcagttagga catatgtttt ggagatattt tatgtggaat ttctccggcc gccaaaacga
     2521 tacgcaaggt aatttttacg atggatataa agatggtaac tgggtaaccg gtattgaagc
     2581 tatcgataaa actaaaaatg aacaattaga ggaagtacca ccttcattaa aaaatgtttt
     2641 aacgcataat aattattaca tgttgccatt tattttaggc atcatgggta tggtttatca
     2701 attgcgcaag gacaaacgag gatttgcagt tgtgcttacc ttattcctgt ttatgggtat
     2761 catgaatatt attaacatga accaaccacc aaccgaacca cgtgaacgtg actacgcaca
     2821 agtaggtgca ttttttgcat ttgccatttg gattggcttc ggtattggtg ctatcatcga
     2881 tttagcaaaa aatttcaaat cgaaaactat tcaggaatat gtattgtata gtgcattgat
     2941 tttattatcc atgtttttcc ttggattaac tatgtattca ttcaccaact tcttaatgat
     3001 tagtgcttat ttaattatcg ttatgttgtt gatgggcggt attgcctacg tgataaataa
     3061 tgccatgaaa aaagaaatgg cacttgcagt ggttgcattt ttattatgta tccctgcacc
     3121 gtttttaatg gcatcacaag gttgggatga tcataatcgt tctgatagaa catttgcccg
     3181 cgacattgcc agaaattatc tggaatcttg tccgccaaat gccatcttat ttacacaagg
     3241 tgataatgat acttatccgt tatggtatgc tcaggaagtt gaaaacattc gacctgatgt
     3301 taaaattatt aacttgagtt tattgggtgt cgactggtat attaatcagt tacgtcatgc
     3361 aacaaacgaa tcggcaccta ttgctctaac attatctgcc gacaaaatat taggtgataa
     3421 acgcaatcag gttgtagata acgataagag taaattcaaa aaccaaacaa tggaattaaa
     3481 agatgcactg gaatttattg gccaggacga tccgaaattt caggtttata gtgaaggttt
     3541 agaagattat tttagttatg tgcctactaa aaaattcaaa gttactattg atgccgaaaa
     3601 aatgaaggct aacaatattg ttccggaagc actagttgag gatatgaaaa aagaactcaa
     3661 ctttgaatta aaacgcggta ctttattgaa aaatgattta atgacattgg acatcgttgc
     3721 aagcaatatt aataaccgac ctatttgttt tgccatcagc gtaactgctg attcacattt
     3781 agggatggaa aaatatttaa tgcaacgcaa tatggtttac cagttaatgc ctgttgagat
     3841 ggaaaacaat aaatcgcaaa tggacggtta tcgcaaacaa atgaataccg atatccaata
     3901 tgatttatta gtaaatcaaa aagataaatt tacttacggc ggtgttgaac gtggtggtga
     3961 aatgtatatc gatccaagtg gaatgggttc agtgttaact acgaagtatt taaattattt
     4021 tgaattggcc aaaaacttag accaggaacg tcagtattta gaagcgcagg ctcgtcaaat
     4081 gatgaccgat acggtaaata aagaatacaa tgaagtaggc cagcagctca tgcaggaaag
     4141 tgctgataaa aagaaaaaaa caatcgaagt attggattta atgcaaactt tatttcctga
     4201 ctccagtgta ccatatgact ttaatatggt gaatgttgcg caggtatatc agcgtttagg
     4261 tgaaaatgaa aaagcaatgg ctattgttac cacaatgaaa gaacgcgtta tgcaggagtt
     4321 agaatattat tataaaatga cattaaaaag ttccttaact gcatctatgt ttgaagatga
     4381 taaattgcgt tctgaaacat gggtttatta tacagtcaac atggcacgca gcgccggtga
     4441 taaagatttt gctgaagcat tagataaaga atgggaaaca ttgcgcatga aatacggaat
     4501 attagcacct aatgagagag gcagaaaata aacgacaacg caaaaaattt gacaatctgg
     4561 tattcggcag acaaccggtt gctgaattac tcaatagcga taaaacagta gataaaatac
     4621 tacttcaaaa ttcggcacag ggcgatattg ttcccctcat caaacaaaaa gcaacggaaa
     4681 aagcaattcc actgcaatat gtgccaatag aaaaattaaa tgcattaaca ggaggcaatc
     4741 accagggggt gattgccttt actgctgcaa tcagctttca aaaaattgac gacattttac
     4801 catttatcat agaacatggt gaaacacctt tgttattaat tttagatggc attacagatg
     4861 ttcgcaattt tggagcaata gcacgaacag catgggcaag tggcgtacat gcgattatta
     4921 ttccggctca tagcgcagca cccgtaaaca gcgatgccat ca
//
LOCUS       DAORKW010000247         4943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_750_length_4943_cov_3.911825, whole genome shotgun sequence.
ACCESSION   DAORKW010000247 DAORKW010000000
VERSION     DAORKW010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_750_length_4943_cov_3.911825"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PLB46_10145"
     CDS             complement(<1..290)
                     /locus_tag="PLB46_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HRF76922.1"
                     /translation="MTEKILILDFGSQYTQLIARRIRELNVYSEIVPCTKIPEITDDI
                     KGIILSGSPFSVSQSDKPAFDIEKVLNKRPLLGVCYGAQFIAQHFGGKVQPSS"
     gene            2062..3096
                     /locus_tag="PLB46_10150"
     CDS             2062..3096
                     /locus_tag="PLB46_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRF76923.1"
                     /translation="MMFKAKFTVVYATMFCLLGISFLNFKSVAPVEDYQPDAYVYEVL
                     RDLGATLPAYYPEKLNPEDVQRGKDIVFFGRTKNPKGGKSTYISLYYMCTNCHNTVME
                     DPNLANNSDPEARLDFAIKNNIPFLQGTTFYGIANRETWYNDDYYKKYGDLVKPANHD
                     LRQAIQLCGKECSKGRYLEDWEETAVLAYYLSISYRLQDILTTEQIAELNSKKRNASQ
                     HPELIKWIKSFYALKSPAAFVEPPYDQGNSLPVGDPARGKAIYELSCQTCHKAYGCSD
                     NVFDNSSFTFKKFNRDFDKDYWWLFKSVRHGYYGYPGHDPYMPHYTAERMSDKQVMDL
                     KAYFQQQASS"
     gene            3183..3638
                     /locus_tag="PLB46_10155"
     CDS             3183..3638
                     /locus_tag="PLB46_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76924.1"
                     /translation="MLNTAYKLSASDEELAYNLGLWCNWIKETDAQLSFFLKDEMNRL
                     QKNFIDNPAEDYFLLNKTLNRKFRYYFWGMLALGLIILYYGVSIQISGFQTYAMGYRL
                     LPVIKNGLTIGFIGMLISIAAIVNLRDDIKRKKILPFGKINFHVLNCCK"
     gene            complement(3699..4388)
                     /locus_tag="PLB46_10160"
     CDS             complement(3699..4388)
                     /locus_tag="PLB46_10160"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRF76925.1"
                     /translation="MYKLMLIIVVITTITSGCNVQSKNAAPLWEVNPLLYAQSDLNVA
                     DDKSFGSRAPAERLLTYDATISIEINAFNDTIIEYFKSTAKKYGGYLSASTEYRSTIQ
                     VTAQNLDAALNDLAILGKVQSKSVSGNDVTEEYADLEIRLENAQAARKRYLELLAQAE
                     NVSAALMVEKELERLNKEIDLLEGQKRYLEQNINFSSITIYLSEKVKPGVLGYIGIGL
                     YEGVKWLFVRN"
     gene            complement(4393..>4943)
                     /locus_tag="PLB46_10165"
     CDS             complement(4393..>4943)
                     /locus_tag="PLB46_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76926.1"
                     /translation="LLLESFRLFMHIFCSFSGQFAYFRANMNSYEQAYLDKKANEDPR
                     EAILLKAERKIKDARLILYILGGIMLVFGFLFYFLEKMLLIDFIFNIVYGVVFFVLAF
                     FVERYPKPIIITSIVLYALKIALAAIVSPASLLSGIILKVIFFAALYKGYQAADEVAD
                     LRRELGLMDEKEMPDQPIDLIK"
BASE COUNT         1699 a          837 c          900 g         1507 t
ORIGIN      
        1 gaggatggtt gaactttacc tccaaaatgt tgagctataa actgagcacc ataacatacg
       61 cctaataagg gacgtttatt gagcactttt tctatgtcga aagccggttt gtcggattgt
      121 gaaacagaaa aaggacttcc ggataatatg atacccttga tgtcatctgt aatttcagga
      181 atttttgtgc agggaacaat ctcactgtaa acgtttaact ccctgatgcg acgcgcaatt
      241 aactgtgtat attgagaacc aaagtctaga atcagaattt tttcagtcat gcgcaaagtt
      301 acgaatttga caatagctta aaatagttaa attgtggaaa gtgatgggca tgctaaaaca
      361 tttcatttaa tttcatatat atcaccataa aatgagtagc cgactattat ctatcaatga
      421 caaagaataa gcagcttaat ttggaacagg tacctggatt tatacctaaa atgctaaaaa
      481 atgtgacttt tttaggcttg agtctatatt ttgatacctt tttatgaagt ggggaatttc
      541 atactgtgac atgtggggaa attttcccca aagtgtgtaa ctaaattcca caactaattc
      601 cccactttgg gaagattaat taaatcttat tgtaaaattt acaattacca atcaacaaag
      661 cctctgccaa tgtaaagctg tacgaatgcc ttaaacagcg gggtttgcaa gcaaccatgc
      721 aggtatatga ataaaaaatt tggtgtaata atttgaaaat gaaggagtta ggggtaaacg
      781 aacaatatac tttaaaacat tacaaaaaaa aagcagagat caccgttaag gttccctctg
      841 ctatccccat gaaaacacac acgtagtcta taattcaaat tgtgtgccag tcagtggaat
      901 agctactggg atggcagttt caggatgtct tggaaaattg gtgtattgaa gattggggaa
      961 tttgaggatt acgataattg caaaaaacaa aaaagcagag tcaacttttt gaagtttact
     1021 ctgctatccc catgaaaaca cacacgtagt ctataaagca aattgcatgc caatcgctgg
     1081 aagggctagt gggatggcag tttcaggacg tcttggaaaa ttggtgtatt gaagattggg
     1141 gaatttgagg attacgataa ttgcaaaaaa caaaaaagca gagccaacta tttaaagttt
     1201 actctgctat ccccatgaaa acacacacgt agagtgtaat tcaaattgcg tgccagtcgt
     1261 gggaatggcc actgggataa tgatttcgga atataattgc aaatttggaa attgaagatt
     1321 gggggatttt gggttttcga ccatgaaata cttgcattag tatttgtgta aaacaaaaaa
     1381 gcagaagcaa ccattcaggc tacctctgct atccccatga aaacacacac aattatacta
     1441 tatgcaaacg gtgtgccaaa tcaaattaat gcaggaaaag tactttagta gtgcaaattt
     1501 tgcagaaagt gactttaatt cacggcaatt attaaaaaaa gaggtggaaa tttgaatttt
     1561 tccaaatatc aatatttata tgtatttcac atacaattga ttaagtcctg tcatattgaa
     1621 aagtcattga ggaacaacga gtattatcta ccttttaaca ggctaatccg acataactaa
     1681 aaatatttaa ccgggatgga gcggattcgt tttgctaatt attaataatg aaggggaaat
     1741 ctcctcctaa acatatagat aaattagtaa aattttcgga aaaatgggta attgaggaaa
     1801 tagcaataga gcacataaag ccaacaaaaa agcagagtgc acggaaggac tccgattcct
     1861 ctgctatccc catgaaaaca caaatcttta tatgagaacg gctaagccga tatcaaattt
     1921 tcattttaaa aaagaactgc tgtaatagtg ttacaaatgt aatctacacg ctgcgaattt
     1981 tccaaatttt tttcgaaaaa atcaaaaatt tgtcttttta taagatggta tagaatgtgt
     2041 aactttgaag taataataac aatgatgttt aaagcaaagt ttaccgtggt atatgccacc
     2101 atgttttgtc tgttaggaat ctcattcctc aattttaaaa gtgttgcgcc agttgaggat
     2161 taccaacctg atgcctatgt gtacgaagta ttgcgcgatt taggtgcaac attaccggcc
     2221 tattatcccg aaaaattgaa tcctgaagac gttcaacgag gtaaggatat cgtgtttttt
     2281 ggtcgtacaa aaaacccaaa aggagggaag tcgacctaca taagtttata ctacatgtgc
     2341 accaattgcc acaatacagt aatggaggat cccaatctgg caaacaacag cgaccctgaa
     2401 gcaaggttag actttgccat taaaaataat attccgtttt tacagggcac caccttttat
     2461 ggtattgcaa atcgcgaaac atggtataac gacgactatt ataaaaagta tggggattta
     2521 gtgaaacctg ctaatcatga tttacgccaa gcgattcaac tttgcggaaa agaatgttca
     2581 aaaggtcgtt atttggaaga ttgggaagaa actgccgtgc ttgcttatta tttatctatt
     2641 agttatcgct tgcaagatat tttaactact gaacaaatag cggaactcaa cagcaaaaaa
     2701 aggaatgcat cacaacatcc tgaattaatt aaatggatta aaagttttta tgcattaaaa
     2761 tcacctgctg cttttgttga accaccttac gaccagggta atagtttacc tgttggtgat
     2821 cctgcaagag gtaaagcaat atatgagttg agttgccaaa cttgtcacaa agcgtatggc
     2881 tgcagcgata atgtttttga taattcctct tttacattca aaaaatttaa tcgcgatttc
     2941 gataaagatt attggtggtt atttaaatcg gttcgtcacg gatattatgg ttatcctggt
     3001 catgacccat atatgccaca ttacactgca gaacgtatga gcgataaaca ggtaatggat
     3061 ttaaaggcgt atttccagca acaagccagt agctgattgg atacactgta tcgaaatata
     3121 aaatctgata ttattgaaat tcaatcggag ctattgaaga tggaaaaaaa atccatgctc
     3181 aaatgctgaa cactgcttat aaactaagtg cttctgatga agagctcgca tataatttag
     3241 gtttatggtg taactggata aaagaaactg acgctcaatt aagttttttt ctgaaagatg
     3301 aaatgaaccg acttcaaaaa aattttattg ataatccggc tgaggattat tttttgctga
     3361 ataaaacctt aaaccgtaaa ttcagatatt atttttgggg tatgctcgca ctgggattga
     3421 ttattctata ttatggagtt tctattcaga tttcaggttt tcaaacttac gctatgggat
     3481 atcgattatt accggtgata aaaaatgggc taacaatagg ttttataggt atgttgatta
     3541 gtatagccgc tattgtcaat ttgcgtgatg atataaaaag aaaaaaaata ttaccgtttg
     3601 gtaaaataaa ttttcacgta ttgaattgtt gtaaataata attgacaacc aattattcat
     3661 ctacctaaaa aacaattatg taggaaatat gctccggatt aattgcgaac aaacaaccat
     3721 tttacacctt cataaagtcc aatgccaata tatcccaaca caccgggttt tactttttca
     3781 ctcaagtaaa tagtaatgga agaaaaatta atattttgtt ctaaatatcg tttttgtccc
     3841 tcaagtaaat caatttcttt attcagtcgt tcaagttctt tttcaaccat taatgcggct
     3901 gaaacatttt cggcttgtgc cagtaattcc aaataacgtt tacgcgcagc ttgtgcattt
     3961 tccagtcgaa tttctaaatc tgcatattct tcggtaacat catttcccga cacacttttt
     4021 gattgcactt tgcctaaaat tgctaaatca tttaatgcag catcaaggtt ttgtgcagta
     4081 acttgaatag tgctgcgata ttcagtactt gcagaaaggt aaccaccata ttttttggct
     4141 gtagatttaa aatactcgat gatagtatca ttaaacgcat ttatttcaat gcttatcgta
     4201 gcatcatacg tcaataaacg ctcagccggc gctctggaac caaaactttt atcgtcagcg
     4261 acattaaggt cactttgcgc atataataat ggatttactt cccataaagg agcagcgttt
     4321 ttactttgga cattacatcc ggaggtaatc gttgtaatta caactattat cagcatcaat
     4381 ttatacataa gcttatttaa ttaaatctat aggttggtcg ggcatttctt tttcatccat
     4441 taatcctagt tccctgcgca aatctgcaac ttcatcagct gcctgataac ctttgtacaa
     4501 cgcagcaaaa aatatcactt ttaaaataat gccagataac aaacttgcag gagaaactat
     4561 ggcagcaagt gcaattttta gcgcatatag aacaatactg gtaatgataa tgggctttgg
     4621 atatcgttct acaaaaaatg ctaatacaaa aaacacaaca ccataaacaa tattaaaaat
     4681 gaaatcgatg agcagcattt tttccagaaa ataaaataaa aaaccgaaca ccagcataat
     4741 accgccgagt atatataaaa tcaatctggc gtctttaatt ttgcgttcgg cttttagcaa
     4801 gatggcttct cgcggatctt cattagcttt tttgtcgagg taagcctgtt cgtagctgtt
     4861 catatttgca cgaaaatagg caaattgccc agagaatgag caaaaaatgt gcataaaaag
     4921 cctgaaggat tctaaaagca act
//
LOCUS       DAORKW010000248         4930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_751_length_4930_cov_5.244718, whole genome shotgun sequence.
ACCESSION   DAORKW010000248 DAORKW010000000
VERSION     DAORKW010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_751_length_4930_cov_5.244718"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..759
                     /locus_tag="PLB46_10170"
     CDS             208..759
                     /locus_tag="PLB46_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF76927.1"
                     /translation="MSDNEIVQLCLNPSTREQGYKILVNVYAKKIYWHVRRMVTDHDD
                     ANDVVQNCFVKIWKGLPDFRQDSKLFTWVYRIATNESITFINEKYRRSSITLETEGES
                     YTSHLKTDPWFDGDETKRQLQLAIDSLPDKQKQVFVMRYYDEMKYEEMEAILGTTTGA
                     LKASYHHAVKKIEIFLKERLNQT"
     gene            841..1428
                     /locus_tag="PLB46_10175"
     CDS             841..1428
                     /locus_tag="PLB46_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76928.1"
                     /translation="MEPINDKLNQQLKEQQLLVPEGYFEQLEQDIFKATTGVKQIEIT
                     ARKQPTLIKRLIIVTAVAASIALVYIGVNLFSKDKITRLGVQFAEMTDAEINNYMDEQ
                     IASLSYDDLYGYLNTNVDDLSTDVLFASTYSDAGQIDESITGELHEQVLDQDVMNSVI
                     DQSPIMLDETIMDSIDNELLQEYFNDATLFENLGL"
     gene            1462..1968
                     /locus_tag="PLB46_10180"
     CDS             1462..1968
                     /locus_tag="PLB46_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76929.1"
                     /translation="MKRTIILTLLITVAAAGFSQGPPGPPPFDEEKIEALRIAFLTRY
                     LDLSSDEAQKFWPVYNKMQDELKVILDKEKDLRNGKKIDEMTDEELAKMINSHFDNEQ
                     KLLDIKKKYAEDFKKVLSLKKVALLADAENEFKREMLKHARDKQGPPPGGGKPNGGEP
                     DAPRRDKP"
     gene            2130..2792
                     /locus_tag="PLB46_10185"
     CDS             2130..2792
                     /locus_tag="PLB46_10185"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HRF76930.1"
                     /translation="MPYNTQLPKLILREYGRHIQQLVDFAVKIEDDEVRNKLVLDIID
                     MMGTMNPTLRNVEDFRHKLWDHIYMMSDFKLKANSPYPVPERETLMRKNIKLKYPKQK
                     IRFAHYGKNIESCVNKASEIEDPAVKQEFAQVIGNYMKLVYQNWNRDNVNDLTIKQDL
                     ESLSNGKLQLDEDSDLDSLTKSTARRQENKPQQQNRNNNGGRNFQRHGQNKNYKNFKK
                     NR"
     gene            2792..4084
                     /gene="murA"
                     /locus_tag="PLB46_10190"
     CDS             2792..4084
                     /gene="murA"
                     /locus_tag="PLB46_10190"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRF76931.1"
                     /translation="MNSFEITGGVKLKGEIVPQGAKNEALQVICAVLLTNQKVTIYNI
                     PDIRDVNFLIELVAQMGVKVNRIDAHTCEFQADNINLAYINSPEFVDQSRKMRGSVMV
                     MGPLLARFKHATLSKPGGDKIGRRRLDTHIIGFMKLGAQFNVETGAISAEKLTGAYML
                     LDEASVTGTANIVMAAVLAEGRTTIYNAACEPYLQQLCDMLVQMGAKISGIGSNLLVI
                     DGVTELSGCSHKILPDMIEIGSFIGMAAMTQSEITIKDVGYDKLGIIPEVFSKLGIKM
                     ELRGDDLYIPSQEVYEIQNFLDGSILTVSDAPWPGFTPDLISIVLVVATQARGSVLIH
                     QKMFESRLFFTDKLIEMGAQIILCDPHRATVIGLDRKNKLRGIEMTSPDIRAGQALLI
                     AALSAEGKSIIRNIEQIDRGYQYIDKRLQALGAQIKRI"
     gene            4254..4775
                     /locus_tag="PLB46_10195"
     CDS             4254..4775
                     /locus_tag="PLB46_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF76932.1"
                     /translation="MKTQGLVIGIFMSVLFISCKTPAPVIGVLSGNIAPEISAVNSTG
                     STHKLSDSKGKLILVEFWDSYNTIGRKNHFEMQRMYGKYKDVEFKEGTGFEIFSLSID
                     ADKAAWSKAVKEDGITWPIIVNDSKAWNSPAVLDYKIASLPKYFLVNDQGIIINHNII
                     ISDLEKILESLKD"
BASE COUNT         1700 a          878 c          975 g         1377 t
ORIGIN      
        1 agttgtatca gaaaataacc cggctccaat cgagtatgta ggtgttaacg ataaattcgg
       61 cgaaagcgga accaccgagc agcttttaac caaatatggt atgagcaccc aacatgttgt
      121 tgacgcagca ctcaaagtaa tagcacgcaa gtaattgcgt ttatgattaa actttatttt
      181 gaagtttgat cgctaacttg gaggtgaatg agcgataacg aaattgttca actttgtctt
      241 aatccatcaa cacgtgaaca aggatataaa atcctggtaa atgtgtatgc caaaaaaata
      301 tattggcatg tgcgtcgcat ggttactgat catgacgatg caaatgacgt tgtgcagaat
      361 tgttttgtaa aaatctggaa aggattaccg gattttcgac aggacagcaa attatttacc
      421 tgggtttatc gcattgctac aaacgaatcc attacgttta ttaacgaaaa atatcgcagg
      481 tcaagcataa cacttgaaac tgaaggcgaa agttatacat cacatctaaa aaccgaccca
      541 tggtttgatg gtgacgaaac aaaacgacaa ctacaattag ctattgacag ccttccggat
      601 aaacaaaaac aggtatttgt aatgcggtat tatgatgaaa tgaaatatga agagatggag
      661 gccattttag gcactacaac aggtgcactt aaagccagtt accatcatgc tgtcaaaaaa
      721 attgaaatat ttttaaaaga acgattaaac caaacataat taggcgtgtc atacttacag
      781 ttagcaatta aaatgtattg ttataactga aatgacaatt attttaataa aaaaattatc
      841 atggaaccaa taaacgacaa actgaaccaa caactcaagg agcagcaatt acttgttcct
      901 gaaggttatt ttgaacagtt ggagcaggat atttttaaag caactacggg cgttaaacaa
      961 attgaaatta cagcacgtaa acaaccaact ttaattaagc gattaattat tgtaacagct
     1021 gttgctgcgt caattgcatt agtatatata ggtgttaacc tttttagtaa agataaaatt
     1081 acaaggttag gtgttcaatt tgctgaaatg acagatgcgg aaatcaataa ttatatggat
     1141 gagcaaattg catctttaag ttatgatgat ttatatggtt atttaaacac caatgttgat
     1201 gatttgagca cagatgtttt attcgcttca acttacagcg atgccggaca aattgacgaa
     1261 tcgataaccg gcgaattaca tgaacaggta ttagaccagg atgtgatgaa ttcagtaatt
     1321 gatcaatctc caataatgtt agatgaaaca attatggatt caattgataa tgaattattg
     1381 caggaatatt ttaatgacgc aactttattt gaaaacttag gattataata taaaaacgaa
     1441 tttaattaaa ctaataatat tatgaaaagg acaatcattt taacactgct aatcactgtt
     1501 gctgcagcag gattttcgca gggacctccc ggaccaccac cttttgatga agaaaaaatt
     1561 gaagcactaa gaattgcctt cttaacaagg tacttagatt taagctcaga tgaagcgcaa
     1621 aaattttggc cggtgtataa taaaatgcag gacgaattaa aagtaattct ggacaaagaa
     1681 aaagatttgc gcaatggcaa aaaaatcgat gaaatgacag atgaagaatt ggccaaaatg
     1741 attaattcgc actttgacaa tgaacagaaa ttgctggaca ttaaaaagaa atatgctgag
     1801 gattttaaaa aagtgctcag ccttaaaaaa gtggcgcttt tagctgatgc agaaaatgaa
     1861 tttaaacgag aaatgctaaa acatgcccgc gacaaacaag gcccccctcc gggtggtggt
     1921 aaaccaaatg gtggtgaacc cgatgcacca cgtcgcgaca aaccttaaag taatagatac
     1981 aggtagtgat agtacaattt ggttttcagg ttccaaaacc gcctataaca gggcggtttt
     2041 ggcttttcaa caagttgtgg aggctccctt catatagcac ttgcaacatg cgtcataaaa
     2101 acataccttt gccttaaaca ataagttaaa tgccatataa tacccagctt ccaaagctga
     2161 ttctgcgcga atacggacgt catattcaac aattagtgga tttcgccgta aaaattgaag
     2221 atgatgaggt gagaaataag cttgttctcg acataattga tatgatggga acaatgaacc
     2281 ctacattaag aaatgtggaa gacttccgtc ataaattatg ggatcatatt tatatgatga
     2341 gtgattttaa acttaaagct aattcacctt acccggtacc ggaacgtgaa actttaatgc
     2401 gtaaaaacat caaacttaaa tacccaaaac aaaaaattcg ttttgcacac tatggtaaaa
     2461 atatcgagtc ttgtgtaaat aaggcttccg aaattgaaga cccggcagta aaacaagaat
     2521 ttgcgcaggt aattggtaat tacatgaaac tggtttacca aaactggaat cgcgataacg
     2581 ttaatgacct caccattaaa caagacctgg aaagtttaag taatgggaaa ttacaattag
     2641 atgaagacag cgatttagat tctctcacaa aatctaccgc ccgccgtcag gaaaacaaac
     2701 cgcaacaaca aaaccgcaac aataatggtg gtcgcaattt ccaacgtcac ggtcaaaaca
     2761 aaaattataa gaatttcaaa aagaatcgtt gatgaactca tttgaaatta ccggaggcgt
     2821 aaaactcaaa ggagaaattg tccctcaagg tgccaaaaat gaagctttac aggtaatctg
     2881 tgctgtttta ttaacaaatc aaaaagttac aatttataat atccccgata tcagagatgt
     2941 taattttctg attgagttag tggctcaaat gggcgtaaaa gttaatcgga tagatgctca
     3001 cacttgtgag tttcaagccg ataatatcaa cttggcctat attaatagcc ctgaatttgt
     3061 tgatcaaagc cgcaaaatgc gcggttcagt tatggtaatg ggcccattgc ttgccaggtt
     3121 caaacatgcc acattgtcaa aacccggcgg tgataaaatc ggtcgtcgca gactggatac
     3181 ccatattatt ggtttcatga agttaggcgc tcaatttaat gttgagactg gtgccattag
     3241 tgccgaaaag ttaacaggtg cttacatgct attggatgaa gcttctgtca caggaacagc
     3301 gaatattgta atggcagctg tacttgctga aggccgcacc accatttata atgctgcttg
     3361 tgagccttat ctgcagcagt tgtgtgatat gttggtacaa atgggcgcca aaatttccgg
     3421 tatcggctct aatttattgg tgatagatgg agtgacagaa ctttcgggtt gtagtcacaa
     3481 aatcctgcct gatatgatag aaattggcag ttttattgga atggctgcca tgacacaaag
     3541 tgaaattacc attaaagatg ttggttacga taaattagga attattcctg aggtgttctc
     3601 aaaactaggc atcaaaatgg agcttcgtgg tgatgatttg tatatccctt cgcaggaagt
     3661 ttatgaaatt caaaactttc ttgacggctc aattctaacc gtttcggatg caccttggcc
     3721 tggatttact cccgatctta tcagcatcgt tttggtggtg gcaacccaag ccagaggtag
     3781 tgttttaatc caccaaaaaa tgtttgaaag ccgcctgttt tttaccgata aactcattga
     3841 aatgggcgcg caaataattc tctgtgatcc acaccgggca acggttatag gtcttgacag
     3901 gaaaaataaa ctgcgcggaa tagaaatgac ttctcctgat attagagcgg gacaggcact
     3961 tttaattgca gcactttccg ctgaaggaaa aagtattatc agaaatatcg aacaaatcga
     4021 cagaggctac cagtatatag ataaacgctt acaagccctt ggagcgcaga taaaaagaat
     4081 ttaatgtcac ttatgcaacg gaatctggca gttattaatc tataactgtt gaaaaggcaa
     4141 tttcacaaaa tacccatcta gtgtaaattt gccgtttctt atttgttaac agccattccc
     4201 aaacaacgac acagcacaac ggttagatgg cgaacattaa agggtttcct tttatgaaaa
     4261 cacaaggatt agttataggc atttttatgt ctgtgctttt tatttcatgt aaaactccag
     4321 cacctgtaat tggcgtgctt tccgggaata ttgctccgga aatttcagcc gtgaacagta
     4381 ccggttcaac acacaaattg tcagattcaa aaggtaaact cattttagtg gagttttggg
     4441 attcctataa taccataggc cgaaaaaatc acttcgaaat gcaacgtatg tatggtaaat
     4501 acaaagacgt tgaatttaaa gagggtacag ggtttgaaat ttttagttta tctattgatg
     4561 ccgacaaagc agcctggagc aaagccgtga aggaagatgg aataacgtgg ccaattattg
     4621 taaacgattc aaaggcttgg aattcaccgg ctgttttaga ctataaaatc gcctctttac
     4681 caaaatattt tttagtcaat gaccagggca taatcatcaa ccataacatc atcatatccg
     4741 acctcgaaaa aattttagaa agcttaaaag attaaattaa atgatgaact cctctccttt
     4801 atcacttaaa aacaccgttg taaacctgat gtttgttggt attacagcca tgtctctcac
     4861 cattgcaggt tgcgccggag cagggtccac tgcacagcac gtaggtgtag caacgggtaa
     4921 tgcaactcct
//
LOCUS       DAORKW010000249         4896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_759_length_4896_cov_4.843421, whole genome shotgun sequence.
ACCESSION   DAORKW010000249 DAORKW010000000
VERSION     DAORKW010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_759_length_4896_cov_4.843421"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PLB46_10200"
     CDS             <1..875
                     /locus_tag="PLB46_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRF76933.1"
                     /translation="VGLSVVIGSLSHYVRALRWKMMLQPLGYNPRISTTFYSVMVAYL
                     ANMAVPRLGEITRCGIVQRYEKVPFDKAVGTIVVERSIDLVMLFLVTFLLFVTQFTLI
                     YDFFQDKVIIPIMEKFSSGLNMLLILGGGLLLLVLISIFLVRRFKHTETYLRIRIMIM
                     NVRDGIYSIRHLKNFKLFIFYSVFIWVCYFAMVWVCFYALEQTAHLGLPEALAVLVFG
                     TVGIISTPGGIGAYHLIVTETLVALYGLDRTYAISFSWLAWSAQTVMIIFMGVISLLL
                     LSRVTRKETIDETV"
     gene            862..1350
                     /gene="rfaE2"
                     /locus_tag="PLB46_10205"
     CDS             862..1350
                     /gene="rfaE2"
                     /locus_tag="PLB46_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HRF76934.1"
                     /translation="MKRSEHIPGKIYDEKTMLQKIAVWRFQGRKIVFTNGCFDILHKG
                     HLHVLNSCADLGYNVAVIVGLNTDASVKKLKGESRPVNNENDRALVLANLVCVDGIVL
                     FDTDTPYDLIKNIQPDFLVKGGDYKANEIVGADIMQANGGSVVIVPYLNNYSTTAILE
                     RK"
     gene            1387..2526
                     /locus_tag="PLB46_10210"
     CDS             1387..2526
                     /locus_tag="PLB46_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRF76935.1"
                     /translation="MYFELTEEQLMIKQAARDFAQNVLKPGVIDRDREMRYPYEEVKQ
                     MGELGFLGMMVSPEYGGGGMDTMSYVLAMEEISKVDSSCSVIMSVNNSLVCWGLEQFG
                     TEEQKRKYLPDLATGRKIGAFCLSEPEAGSDATSQRTTAEDKGDYYLLNGTKNWITNG
                     GTASIYLVMAQTDVAKGHKGINCLIVEKDMPGFIVGAKEDKLGIRASDTHTLLFNDVK
                     VPKENRIGEDGFGFTFAMKTLSGGRIGIASQALGIAAGAYELALDYAKTRKAFGKEIS
                     QHQAIQFKLADMATEIEAARLLIYKAAQIKDDHGNYDLASSMAKVFASEVAMKTTVEA
                     VQIHGGYGFVKEYHVERLMRDAKITQIYEGTSEVQRIVIGRSILR"
     gene            complement(2600..3313)
                     /locus_tag="PLB46_10215"
     CDS             complement(2600..3313)
                     /locus_tag="PLB46_10215"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRF76936.1"
                     /translation="MKKLLPTSLVVLLITTLAFACQSFSSKSVTPDTKELNIQPFTEL
                     SVAGSFEVLFTQEANTTARAEGSAEDLEKIEIETNGDKLILKQKKNSNIKNVVIYIST
                     PNINEVGIAGSGTFKSTNTITSTKTLHFGIAGSGTITAAIDAKDVKSEIAGSGDIHLS
                     GKSSNSKIEIAGSGNYKANDLSTGNTEVEIAGSGNAYVNATGKLSAEIAGSGSIYYKG
                     QPSDIDKSVAGSGKIKPMD"
     gene            complement(3499..4884)
                     /locus_tag="PLB46_10220"
     CDS             complement(3499..4884)
                     /locus_tag="PLB46_10220"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRF76937.1"
                     /translation="MTSVIVLAAAGIASMFVGVFNIKRLALPLVLLACLAALGIIMFY
                     YNGGYESLMNNMLAFDAFSYGFSVIMILITMGILVISNFYYRNNVEHLSDIYALFIFS
                     LIGGILLCSYNSLVMLFLGIEILSIPLYILAASNRKNLFSNEAGLKYFLMGSFASCFL
                     LLGITFIYGTANSFDMHVIAQYVNAHPGDLSPLFITGAVLILGSFIFKVSAVPFHFWA
                     PDVYQGSPTVITAFMATVVKTAAFGGLIRFIGGTIFPETAIWSDVLNVIAILTLIVGN
                     ILALYQTNLKRLLAYSGIANAGYILVAVATLREETYGYVLYYLTAYGVGSMIAFTVYF
                     IIKDQTGIDSISGLKGLFSKNKFLATVLAFTMLSLAGIPPLAGFFGKYAIFVNAINAN
                     AIWLVIIAILTSLIGVYYYLRVMTTAMSAGDDIPKLDIHLSYKIVLTLGVIILLTLGV
                     VPDLFNNLISK"
BASE COUNT         1580 a          835 c          947 g         1534 t
ORIGIN      
        1 gggtcggact ttcggtagtg ataggcagtt taagccacta cgttcgggca cttcgctgga
       61 aaatgatgtt gcagccactc ggttacaacc cccgcatttc cactactttt tactctgtaa
      121 tggttgctta tttagccaat atggcagttc ctcgcctggg tgaaattacc cgttgcggta
      181 ttgtgcaacg ttatgaaaaa gtaccatttg ataaagctgt cggcactatt gtcgtggaaa
      241 ggtccattga ccttgtgatg ttatttctgg ttacattttt actttttgtt acgcagttta
      301 ctcttattta tgattttttt caggataagg ttatcattcc aattatggaa aaattttcat
      361 caggcctcaa catgctgctt attttaggtg gtggcttatt gcttttggtg ctgattagta
      421 tctttttagt gcgcagattt aaacataccg aaacgtattt gcgtattcgt atcatgataa
      481 tgaatgtgcg tgatggtatt tattccattc gccatttgaa aaattttaag ttgtttattt
      541 tttattccgt gtttatttgg gtatgctatt ttgctatggt ttgggtatgt ttttatgccc
      601 ttgaacaaac tgcccatctg ggcttaccgg aggcattggc agtgctcgtt tttgggacgg
      661 taggcattat cagcacgccg ggtggtattg gcgcctatca tctgatagtt accgaaacac
      721 tggttgcttt atatggtttg gaccgcacct atgccattag ttttagctgg ctggcatgga
      781 gcgcacaaac tgttatgatt atttttatgg gagtaatttc attattactt ttatcccgcg
      841 ttacgcgtaa agaaacaatt gatgaaacgg tctgaacata ttcccggaaa aatttatgat
      901 gaaaaaacca tgttgcaaaa aattgcagtc tggcgttttc aaggcagaaa aattgtgttc
      961 accaatggct gttttgacat cttacataaa ggtcacttgc atgtattaaa cagctgtgcc
     1021 gatttaggtt ataacgtagc cgtaattgtc ggattaaata cggatgcttc ggttaaaaaa
     1081 ttaaaaggtg aatcacgacc ggtaaataat gaaaacgatc gcgcattggt tttagctaat
     1141 ttagtttgtg tagatggcat tgtgttattt gataccgata caccttatga tttaatcaaa
     1201 aatatccaac ccgatttttt agtaaagggt ggtgattaca aagccaatga aattgttggc
     1261 gcagatatta tgcaagcaaa cgggggttca gttgttattg ttccctattt aaataattat
     1321 tcaacaacag caatactcga gaggaaataa atacagacca ttatttttaa accacaattg
     1381 aattttatgt atttcgaatt aacagaagaa caactcatga ttaaacaggc ggcaagagat
     1441 tttgcgcaga atgttttaaa accgggcgta attgatcgcg accgcgaaat gcgttatccg
     1501 tatgaggaag ttaaacaaat gggtgagctt ggatttttag gaatgatggt atcaccggaa
     1561 tacggtggtg gtggaatgga tacgatgagt tatgttttag cgatggaaga aatttcgaaa
     1621 gtagacagct catgttctgt gattatgagt gtaaataatt cattagtatg ttggggtctt
     1681 gagcaatttg gtacggaaga acaaaaaaga aaatatttac cggatttagc aacaggaaga
     1741 aaaattggtg ccttttgttt gagcgaaccg gaagcaggga gtgatgcaac atcacaacgc
     1801 acaacagctg aagataaagg tgattattat ttattaaacg gcactaaaaa ctggattacc
     1861 aatggtggta ctgccagtat ttatttagtg atggcacaaa ccgatgtggc taaaggacat
     1921 aaaggcatta actgcttaat tgttgaaaaa gatatgccgg gctttattgt tggtgctaaa
     1981 gaagataaat taggcatcag agcaagcgat acacatacct tattatttaa cgatgtaaaa
     2041 gtgcctaaag aaaatcgaat tggtgaagat ggtttcggat ttacttttgc gatgaaaact
     2101 ttaagtggtg gtagaattgg tattgcttca caagcattag gtattgctgc aggtgcatat
     2161 gaattagcgt tagattatgc taaaacacgt aaagcatttg gcaaagaaat ttctcaacac
     2221 caggctattc aatttaaact tgccgacatg gccacagaaa ttgaagctgc tcgtttatta
     2281 atttataaag ctgcccaaat aaaagacgac cacggcaatt atgatttagc atccagtatg
     2341 gctaaagtat ttgctagcga agtagcgatg aaaactactg ttgaagctgt acaaattcac
     2401 ggtggatatg gcttcgtaaa agaataccat gttgaacggt taatgcgcga tgcgaaaatc
     2461 actcaaattt atgagggaac aagcgaagtg caaagaattg taattggcag aagtatttta
     2521 agataaatac tacgagatac aaaaagccgc atgttgaata atcagcatgc ggctttttta
     2581 ttgctttttt tcggaataat taatccattg gtttaatttt tccggaacct gctacagatt
     2641 tatcaatatc gcttggctga cctttataat aaatagaacc acttcctgca atttcagcag
     2701 ataatttacc ggttgcattt acataagcat ttccacttcc tgcaatttct acttcggtat
     2761 taccggtgct taaatcgttg gctttatagt tacctgaacc tgcaatttct atttttgaat
     2821 tggaagattt tccggataaa tgaatatcac cgctgcctgc aatttcagat ttaacatctt
     2881 ttgcatcaat agcagcagtt atagttcctg aacctgcaat tccaaaatgt aatgtttttg
     2941 ttgaagtaat ggtattggtt gatttaaatg ttccacttcc tgctatacca acttcattaa
     3001 tgtttggtgt agatatatag ataacaacat tttttatatt cgaatttttc ttttgcttga
     3061 ggataagctt gtcaccattt gtttcaatct caattttttc caaatcttca gcgcttcctt
     3121 cagcacgagc tgttgtgtta gcttcctggg tgaataacac ttcaaaagaa cctgcaacag
     3181 ataattcagt aaatggctga atgtttaatt ctttggtgtc aggggttaca cttttgctgc
     3241 taaatgattg acaggcaaag gcaagggttg ttattagtaa aacaactaaa cttgtaggaa
     3301 gtagtttttt catattgttg attttatgac cttaaattta aggttatttg tcaattcaac
     3361 attaaagaca tggtattttt tccaataatt aagtgatttt aacatattag gcaacttcgg
     3421 aagaacgtaa aagggatgtc acctttccta atagaaagca acgtaacaaa cggtaattaa
     3481 gcaaattacc taactaaatt atttgctaat taagttatta aataaatcgg gaactacacc
     3541 taaggtgagc agaataatta cgcccaaggt caatacgatt ttataactta aatgaatatc
     3601 caatttagga atgtcatcac cggcactcat cgcagtagtc ataacgcgca gataataata
     3661 cacgccaatt agtgaagtta aaatggcaat aattaccaac catattgcat tagcattaat
     3721 ggcatttaca aaaatggcgt atttaccaaa aaatcctgct aaaggcggaa tacctgcaag
     3781 cgacaacatg gtaaatgcca atactgtagc taaaaattta ttttttgaaa acaatccttt
     3841 taatccggat atactatcga ttccggtttg gtctttaatg atgaaataaa cggtaaatgc
     3901 aatcatacta ccaacgccat aagcggtaag ataatataaa acatatccgt aagtttcttc
     3961 acgcaaagta gctactgcca ctaaaatata acctgcattc gcaatacctg aatacgcgag
     4021 taatcgtttt aaattggttt gatataatgc caggatgttg cctactataa gtgttaagat
     4081 ggcaattaca tttaatacat cgctccaaat ggcagtttca gggaatattg ttccgccaat
     4141 aaatcgaatt aatccaccaa acgcagcagt ttttactaca gtcgccataa aggcagtaat
     4201 tacagtaggt gaaccttggt acacatccgg tgcccaaaaa tgaaatggca cagccgatac
     4261 tttaaaaata aatgaaccga gtatcaatac tgctccggta ataaataatg gtgataagtc
     4321 gcccggatgc gcatttacat attgtgcaat tacatgcata tcgaacgaat tagcagtgcc
     4381 gtaaataaat gttatcccta acaacaaaaa gcaacttgca aatgaaccca tcaggaaata
     4441 ttttaatcct gcttcgttgc tgaataaatt tttacggttg ctggcagcta atatgtataa
     4501 tggaatactc aatatttcaa tccccaaaaa caacattacc aaactattgt aagaacacaa
     4561 taagatgccg cctattaaag agaatatgaa taaagcataa atatcactga gatgttcaac
     4621 attattgcga taataaaaat tgctgattac caaaataccc attgtgatta aaatcataat
     4681 cacactaaat ccgtatgaaa atgcatcgaa agcgagcata ttgttcatca ggctttcata
     4741 tccaccattg tagtagaaca tgataatacc taatgctgcc aaacaagcca acaataccag
     4801 aggtaatgcg aggcgtttaa tattaaacac acccacaaac atggaagcga tacccgctgc
     4861 tgctaaaacg attactgaag tcattgataa ttatat
//
LOCUS       DAORKW010000250         4864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_764_length_4864_cov_4.396548, whole genome shotgun sequence.
ACCESSION   DAORKW010000250 DAORKW010000000
VERSION     DAORKW010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_764_length_4864_cov_4.396548"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLB46_10225"
     CDS             <1..242
                     /locus_tag="PLB46_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76938.1"
                     /translation="VSTATVLKEILSIDKRYTYFDPFKNKTIYNNYARVNEMGGNDYW
                     ESAIVYPDKVLNDLVRIFHPELKTGDNYFYYIHLQ"
     gene            239..1264
                     /locus_tag="PLB46_10230"
     CDS             239..1264
                     /locus_tag="PLB46_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRF76939.1"
                     /translation="MNKQRHSVLFVLLSILLLILFFADIFIGSVAISLNEIMQVFTGQ
                     LSKDTPQSIIILQSRLPKALTAILAGCALPVAGLLMQTFFRNPVAGPDILGVSAGSSL
                     LVALVMLTVGTWFSMDVFSNVGIILAAIVGAMLVLFIILAVAARVRDSVTLLIFGLMF
                     GMAISAVVGVLQYYSSQGALKLFIMWTFGSLSGVTWPQLYILAVIVIIGLLLAVTQIK
                     ALNLLLLGEQYATSLGLNIRKSKIFIILTTGLLTGAITAFCGPIAFIGIAVPHLSRMI
                     FKVNSHAIILPASILLGICILLICDIIAQTPGNDGVLPINAITAFMGAPFIIYIIWKN
                     QHLKRVF"
     gene            1231..2043
                     /locus_tag="PLB46_10235"
     CDS             1231..2043
                     /locus_tag="PLB46_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF76940.1"
                     /translation="MEKSTFKTRILMEAKTLLAINGLSVGYPEKVILKDMLLHIPANS
                     IVAVLGLNGKGKSTFIRTLAGLQPALQGDILFNERSLSSYTAIERAQLFSIVLPGRGD
                     VVQGLLVHELFHTTRVTFTNGFGKITEDDIQLINRICTSLHIEHLLHKRIYQLSDGES
                     QLVFIARALIQDTPIIFMDEPTSYLDVVNKAEIFNLIQSLPKQNKTVVFTSHELELAL
                     QIADYCLFIGNEGKHVFGTTTTIIEQGYIPDFFNSKNVQFDQTTGRIKINND"
     gene            complement(2116..2670)
                     /locus_tag="PLB46_10240"
     CDS             complement(2116..2670)
                     /locus_tag="PLB46_10240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF76941.1"
                     /translation="MYFNAIGRPFVGKKIRPLIPLLKKDTPILDLGSGNGLAAHLLKQ
                     QGLNITCVDIHEGEYHPSVKPIVYDGKTIPFPDRHFEAGIILTVLHHIDNPEAVIKEA
                     GRVCDRLIIMEDIYTNKVQQYLTYWLDSLANLFYSPCPHTNKNDKEWKATFEAMDLEL
                     VSVHYRYVLFIVKQVIYEVKHKSA"
     gene            complement(2745..3296)
                     /gene="msrB"
                     /locus_tag="PLB46_10245"
     CDS             complement(2745..3296)
                     /gene="msrB"
                     /locus_tag="PLB46_10245"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRF76942.1"
                     /translation="MKKQLTVLAVIVIPAIFGCGQAKTIQPKTTEQSTSDTTKVAITL
                     EAANYVWDGTKVEKTSEEWKKQLTPMQYHVAREAGTERAFSNAFHDNHEDGIYYCVGC
                     GMPLFDAKHKFDSGTGWPSYYQPINDKNVAFDIDYEIGYERKEVHCARCSTHLGHVFD
                     DAYDQPGGLRYCINSASLIFEKR"
     gene            complement(3407..>4864)
                     /locus_tag="PLB46_10250"
     CDS             complement(3407..>4864)
                     /locus_tag="PLB46_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF76943.1"
                     /translation="IDITPGERMAVLKQNHFEFDQHSVIHTVMMGHKKLWNIIQEKDA
                     LYAKPDFSEEDGIRVGDLEAEFGEMNGWNAESDAAELLSNLGIREELHYEFMENLSGK
                     EKVRVLLAQALYGNPDILLMDEPTNDLDIDTISWLENFLADYQNTVIVVSHDRHFLDA
                     VCTHVADIDYGKITIFTGNYSFWYQSSQLMLQLMAQKNKKTEDKRKELQDFISRFSAN
                     ASKAKQATSRKKALEKLNIEELRPSTRKYPGIFFTPERDLGDQVLQVNQLSKKIGDKT
                     LFHNITFTAEKGDKIAIVSRDPLAKTNFFEIIAGEKEADHGTYKWGVTVTNNYLPNEN
                     HTYFTGDLNLIDWLRQYSKDKDESFVRGFLGRMLFSGEESLKSCKVLSGGEKVRCMIS
                     RSMLLGGNALILDEPTNHLDMESITAFNNALQDFKGFVIFTSHDHQFMQTVANRIIEI
                     TPNGIIDKLMSYDEYIIDPKVKELQNQLYPTEQLV"
BASE COUNT         1441 a          906 c          879 g         1638 t
ORIGIN      
        1 atgtaagcac agcaacagtg ttaaaagaaa ttttatctat tgataaacga tatacttatt
       61 tcgacccatt caaaaataaa acgatttata acaactatgc cagagtaaat gaaatgggtg
      121 gcaacgatta ttgggaaagt gccatagttt acccggataa agtattaaat gatttagtgc
      181 gaatattcca tcctgaatta aaaacaggcg ataattattt ttattatatc catttacaat
      241 gaacaagcaa cgtcatagtg tattgtttgt tttattaagc atattgttgc ttatattgtt
      301 ttttgcagat atttttattg gctctgtagc tatttcgttg aatgaaatta tgcaggtgtt
      361 taccggtcaa ttatcgaagg atacaccgca atctatcatt atattacaat cgcgactgcc
      421 aaaagcatta acagctatat tagccggatg tgcattaccg gtagccggtt tgctcatgca
      481 aacatttttt agaaaccctg ttgcggggcc tgatatttta ggtgtttctg ctggttccag
      541 tttattagtg gcattggtta tgttgactgt tggcacctgg ttttcgatgg atgtatttag
      601 taatgtgggc attattctcg cagcaattgt tggtgctatg ctagtgttgt ttattattct
      661 cgctgttgca gcccgtgttc gtgacagtgt taccttactc attttcggat taatgtttgg
      721 aatggcaata agtgccgttg ttggtgtgtt acaatattat agcagtcagg gcgcattaaa
      781 attatttatc atgtggacct tcggcagttt aagtggcgtt acctggccgc aattatatat
      841 tttggctgta atagtaataa ttggtttgtt gttggctgtc acacaaatta aggcactcaa
      901 cttattatta cttggcgaac aatatgctac atcgttggga ttaaatattc gtaaatcgaa
      961 aatatttatc atcctcacta ccggattatt aaccggagcc atcactgcat tttgtggtcc
     1021 gattgccttt attggtattg ccgttccgca tttgtctaga atgatattta aggtgaatag
     1081 tcacgccatt attttacctg catcaatttt attgggcata tgtattttac ttatctgcga
     1141 tattattgca caaactcccg gtaatgatgg tgtgttacca attaatgcca tcacagcatt
     1201 tatgggtgct ccatttatta tatatattat ttggaaaaat caacatttaa aacgcgtatt
     1261 ttaatggaag caaaaacgct gttggcaata aatggtttga gtgttggtta tcctgaaaag
     1321 gtaatcttaa aggatatgtt attgcatatt cctgctaata gtattgtagc tgtgctcgga
     1381 ttgaatggaa aaggtaaatc cacatttatt cgcacattag ccggattaca acctgccttg
     1441 caaggcgata tattgtttaa tgagcggtca ctttcatctt atacagcaat agaacgagca
     1501 caattattca gtatcgtgtt accgggcagg ggagatgtag tgcagggatt attggtgcat
     1561 gaattatttc ataccacacg tgtcacattc actaatggtt ttggaaaaat tacggaagat
     1621 gatattcaat taattaatcg catttgtact tcactgcata ttgaacattt attacataaa
     1681 cgtatttatc aattgagtga tggtgaatca caattggtat ttattgcaag agcgttaata
     1741 caggacacac caatcatatt tatggacgag ccaacctcat atttagatgt agtaaataaa
     1801 gcagaaatat ttaatttaat tcaatcgtta cccaaacaaa ataaaacggt agtatttact
     1861 tctcacgaat tggaattagc cttacaaatt gcagattatt gtttatttat tggcaatgaa
     1921 ggcaaacatg tattcggcac aacaacaaca attattgaac aaggttacat ccccgatttc
     1981 ttcaacagca aaaacgtcca attcgaccaa accaccggcc gcataaaaat caacaatgat
     2041 taattacctc ccgaaacttc ccgccatccc gtcttcccgg ctaaaaaaaa cacctcaata
     2101 aaaccttaaa agaagttaag ctgatttatg cttcacctca taaataacct gcttcacaat
     2161 aaataacaca taacgataat gtaccgacac taattcgagg tccatagcct cgaaggtagc
     2221 tttccattct ttatcgtttt tattggtatg cggacaagga gagtaaaata aattcgcaag
     2281 tgaatcgagc caataggtta aatattgttg caccttattc gtataaatat cctccataat
     2341 aatcagcctg tcgcaaacgc gacctgcttc tttaataact gcttcaggat tatcaatatg
     2401 atgtaatacg gttaaaatga tacccgcttc aaaatgtctg tccggaaatg gaattgtttt
     2461 cccatcgtac actattggtt ttactgaagg atgatattcc ccttcatgaa tatctacaca
     2521 agtaatattc aatccttgct gtttaagcag atgtgcagct aaaccatttc ctgaaccaag
     2581 gtccagaatg ggagtatctt ttttaagcag tggtataagc ggtcttatct tttttccgac
     2641 aaaaggtctt ccaattgcat tgaaatacat tttgcgaaac agggggactt tatgaatgat
     2701 gtataatacc aattactgcc ttttgaggtt aacaggcagc aaagttatct tttttcaaat
     2761 attaaagaag cagaattgat acaatatctc aaaccacccg gctgatcgta ggcatcatca
     2821 aaaacgtgac ctaaatgcgt actgcaacgt gcgcaatgca cttccttgcg ctcatatcca
     2881 atttcgtagt cgatatcgaa ggctacgttt ttatcgttga ttggttggta ataactcggc
     2941 caacctgttc cggaatcaaa tttatgttta gcatcaaaca ggggcattcc acatcctacg
     3001 caataataaa taccatcttc atgattatcg tgaaatgcat tgctgaaagc gcgttcggtg
     3061 cctgcttctc tcgccacatg gtattgcatg ggcgtgagtt gctttttcca ttcttcgctg
     3121 gttttttcca ctttagtgcc atcccaaaca taatttgcag cttccaaggt tatggccact
     3181 tttgttgtat ctgatgttga ttgttccgtt gttttaggct gaattgtttt agcctgacca
     3241 cagccaaaaa tggcagggat tacgattact gcaagtacag ttaattgttt tttcattgtg
     3301 ataattattg agttgtcaga catataaacg tattttttca acctttggat gtataacaat
     3361 tatccctcag aatagtttca caatgaaaat tgcgatgcat tcaggtttat actaattgct
     3421 cagtagggta tagctggttt tgtagttctt ttacttttgg atcaataata tactcatcgt
     3481 atgacatcaa cttatcaatt ataccattag gggtaatttc gataatacga tttgccactg
     3541 tttgcataaa ctgatggtca tgagaagtaa atatcacaaa tcctttaaag tcctgtaatg
     3601 cattgttaaa ggctgtaata ctctccatgt cgaggtgatt ggtaggttca tctaaaataa
     3661 gcgcattacc acctaataac atactacgtg aaatcataca acgtactttt tcaccacctg
     3721 ataatacttt acagcttttt agagattctt ctccactgaa taacatacgt cctaaaaatc
     3781 cgcgaacaaa tgattcgtct ttatcttttg aatattgacg taaccaatca atcagattga
     3841 ggtcgcctgt aaaataagtg tgattttcgt taggtaaata attattcgta acggttacac
     3901 cccatttata tgtgccatga tcggcttctt tttcaccggc aataatttca aagaaattcg
     3961 tttttgctaa tgggtcgcgg ctaacaattg caattttatc acctttttca gcggtgaatg
     4021 taatattgtg gaataaggtt ttatcaccaa tttttttgga taattgatta acctgtaaaa
     4081 cctggtcgcc caaatcgcgt tccggtgtaa agaaaatgcc cggatatttg cgtgttgaag
     4141 gacgtaattc ttcgatattt aatttttcaa gtgctttttt acgggaagta gcctgtttag
     4201 cttttgaagc atttgcacta aagcggctga taaagtcctg taattctttg cgtttgtctt
     4261 ccgtcttttt gtttttctgc gccattaatt gcaacatcaa ttgcgaagac tgataccaga
     4321 aggaatagtt accggtaaaa atggtgattt taccataatc gatatctgca acgtgtgtac
     4381 aaaccgcatc caaaaagtga cggtcgtggg acacaacaat aactgtattt tgataatctg
     4441 ccaggaagtt ttccaaccaa ctaatcgtat caatatctaa gtcgttggta ggctcatcca
     4501 ttaataaaat atcgggattg ccatataaag cctgagctaa taataccctt actttttctt
     4561 taccgctgag gttttccatg aattcataat gtaattcttc acggataccc aagttgctta
     4621 ataattctgc tgcgtcactt tctgcgttcc agccattcat ttcaccgaat tcagcttcta
     4681 aatccccaac gcgaattcca tcttcttcgc tgaaatccgg tttagcatat agtgcatctt
     4741 tttcttgaat aatgttccac aattttttgt gacccatcat aacagtatga attacagagt
     4801 gttggtcgaa ctcaaagtga ttttgtttta aaaccgccat acgttctccc ggcgtaatat
     4861 cgat
//
LOCUS       DAORKW010000251         4854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_765_length_4854_cov_4.432173, whole genome shotgun sequence.
ACCESSION   DAORKW010000251 DAORKW010000000
VERSION     DAORKW010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_765_length_4854_cov_4.432173"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4854
                     /locus_tag="PLB46_10255"
     CDS             <1..>4854
                     /locus_tag="PLB46_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76944.1"
                     /translation="KSIVCFAYFTFLVQPFFSAGLFAGNGPLQPEFQGPAVTENAQLV
                     DPFTGDFSYSVDILGDVGIPITLNYTAGISMEQDASWVGLGWNFNPGSIMRSLRGLPD
                     DFMGDVLKEDVNIRPNITGGVSFAIGPNEFFGIDKDAVAGAAEGPTSGASIGIELHYN
                     TYTGFGAGTFADAKININESLSLGLSAESGNENSFGGVGISPSFNIAKSLGFTDDNYE
                     KKLGLTLSSEFSTSGGMKYFNVGSFYSKTKTVQLARTAKAGGDNADYTDSKSKNASAN
                     LISFQKSYTPSIGKEFLNQSYSLKGTLGAEVLFSNVATDISGYYTSSTLKDNHTESPA
                     YGYLYLKNSEDNNIVQDINRENDISVVKNSPTLPISYYAYDLFSVSAPGLGGSFRAYR
                     NDIGYMQDEKMKNNTISADLGAEVAAMETFKGGVNVVGVYGESISKPFLDNNIIAEYT
                     PHKTMYSSINNIENNVHFKFVGDNYLDSDPEFIENLQGNSAIETNMRSSFLVPLVNEK
                     KWISNDHNYYPEEIFGGGSHDEDEFDASHSSRNGRVPNAKSIQYFTHEMVEQFFGGEN
                     KYAISPNVKDHHIQRIVVTGEDGTQYVFGLPVYNNEQQEIMFNVGGATAPDTQSDEYL
                     VKYDSDQKSLNNKSGLDHMYQNTTTPAYATSFLLTEVYSPDYVDMQGDGPTDDDQGTY
                     IVYTYGEKEGDGKYHPNMDNFKWRTPMSTEDFYASYIKGQTNRLTDDKASIIYGTKEV
                     WYLKTVETKSHFAEFFILDRADGFTTAGIDGGISNTSDQCQKLLDRIEVYSKFDKIRD
                     AYPPVGEEPATDELSLMDELPLTPVKTINFTYEYFLCKGVPTNPDPAHNGRLTLTKVD
                     YTYYNSNKSKFNYYKFEYNTADFPDGDDADTDPDFEPAYHPLASDKWGTIQPYDPAFP
                     TQVFPYTKQDNRALSDYLAGLWSLRRITLPNGGTIEVDYEADDYAYVQNRKAATAFKV
                     RSCAKGFDEIGDDVIVSNLEEDNAYLYGADVTGENFLVNKYIITFELPEPIEGDMSPT
                     AARNYFKDNYLKDYSGSKHIGDNLYFKFYMGTDNDDDLFVDETPHDIEYRFEYVEGYA
                     SVDYNNVGVLKTIDNEETPYTIGYFKLNPAKVYTAAGDFAASAFKPEDYDDGFFGTKD
                     VNPIIKASWQWTMLNAQQNITDDSGPDESLGALDYLFDLVSKLTMVNTLLTMFMNPNQ
                     LMADGKYGNEFDPEKSIIRLHEPSGFKIGGGHRVKEIRYYDDWRDMTADENPEKSSIY
                     GIKYVYQNPKNDFSSGVAAYEPMAGGEENPLRYPDTYNHYAGDFAYARYLEHPLGESF
                     YPSASIGYDYVEEIPFGQFETAEETWSEQYTDVGKIVRTFYTAKDYPSITKNTELNPI
                     NKKFKPGVFASFFQSTHCDLFAGSQGFVIETNDMHGKPRTEATLSKTGEVISSVEYKY
                     KESAPGVLNNTVNVIDEVGEVTPAEMNVTYDVFTDHRLNTAASLGGGAKLGLDFSLIV
                     PVVEAYPNFVGTKSTTAFSVVTKHVQRSGILSQVIRNTNGSILTSNNLVYDKRTGNPI
                     VVSEQNEFEDTYYVVNIPAHWIYDGMGMANDNWDFKFVSHNNDVTTDDDLFNLSTGQI
                     TSATLLNN"
BASE COUNT         1614 a          757 c          985 g         1498 t
ORIGIN      
        1 aaatcaatag tatgttttgc atattttact tttctagttc aaccattttt ttctgccggt
       61 ttatttgccg gaaatggccc cttacagccc gagttccagg gaccggctgt tacagaaaat
      121 gcacaactgg ttgatccgtt tacgggtgat tttagttatt cagtagatat tcttggtgat
      181 gtgggtattc ctattacttt gaattacact gcgggtattt caatggagca ggatgccagt
      241 tgggttggcc ttggttggaa ttttaatcct ggaagtatta tgcgaagttt gagaggatta
      301 ccggatgact ttatgggtga tgtgctaaaa gaagacgtga atattcgtcc aaacattaca
      361 gggggtgtat catttgctat aggaccaaat gaattttttg gaatagacaa ggatgctgtg
      421 gctggagcag ctgaaggccc aacttcgggc gccagtattg gaattgaact gcattataat
      481 acttatacag gatttggtgc gggaaccttt gctgatgcca aaattaacat aaatgaaagt
      541 ctcagtttag gattatctgc tgaaagcggt aatgaaaata gttttggtgg agtaggtatt
      601 agtcctagtt ttaatattgc caaaagtttg ggatttacgg atgataatta tgaaaaaaaa
      661 ttggggttga cattatcttc tgaattttca acatcagggg gtatgaaata ttttaacgtg
      721 ggttcattct attccaaaac aaaaactgtg caattggcta ggactgctaa agctggcggg
      781 gataatgctg attatactga ttcgaaatct aaaaatgcaa gtgctaattt aattagtttt
      841 caaaaatcgt atacgccgtc aatcggtaaa gaatttttaa atcagtcata ctcattaaaa
      901 ggtactttag gagctgaagt cttgttttcg aatgtagcaa ccgatatcag cggctattat
      961 acctcttcaa cattaaagga taaccatact gaatcaccag catatgggta tttatattta
     1021 aaaaactcag aggataacaa tattgtacaa gatataaatc gggaaaatga tatttctgtt
     1081 gttaaaaatt cgcctacatt gcccatcagc tattatgctt acgacttatt tagtgttagt
     1141 gcacctggtt taggtggaag ttttagagca tatagaaatg acataggtta tatgcaggat
     1201 gagaagatga aaaacaacac catttctgca gatctaggtg cggaagttgc tgctatggaa
     1261 acatttaagg gtggtgtcaa tgttgttggt gtttacggag agagtatcag taaaccgttt
     1321 ttagataata atataatagc tgaatatact ccacacaaaa ccatgtattc atctattaat
     1381 aacattgaaa ataatgttca tttcaaattt gtaggtgata actatttaga ttcagaccct
     1441 gaattcatag aaaatttaca agggaattct gctattgaaa ctaatatgcg tagctccttt
     1501 ctagtgcctt tagtaaatga aaaaaaatgg atttccaacg accataatta ttatccggaa
     1561 gaaatttttg gaggtggttc acatgatgaa gatgaatttg atgcttctca ttcatcaaga
     1621 aatggtcgtg ttccaaatgc aaaatcaatc caatatttta ctcatgaaat ggtcgaacaa
     1681 ttttttggag gcgaaaataa gtatgccatt tctcctaatg taaaggacca ccatattcaa
     1741 aggatagtgg ttacaggcga agatggtacg caatatgttt ttggtttgcc tgtttataat
     1801 aacgaacaac aggaaattat gtttaacgtt ggtggagcta ctgctccaga tactcaatct
     1861 gatgagtatt tagtgaaata tgatagtgat caaaaatcac ttaataataa atccggattg
     1921 gatcacatgt accaaaatac tactacacct gcttatgcaa ctagtttttt gttaactgag
     1981 gtgtattctc cggattatgt tgatatgcaa ggtgacggac ctactgatga tgatcagggg
     2041 acttatattg tttataccta tggggaaaaa gaaggtgatg gcaagtatca cccaaatatg
     2101 gacaatttca aatggcgaac accaatgtca actgaggact tttatgcaag ttatattaaa
     2161 ggtcaaacta atcgtttgac tgatgacaaa gcaagtatta tatatggtac aaaagaagtt
     2221 tggtatttga aaacagttga aacaaaatct cacttcgcgg aattttttat tttggataga
     2281 gccgacgggt ttactactgc aggtatagat ggtggtatta gtaatacttc tgatcaatgc
     2341 caaaagttac ttgatagaat agaagtttac tcaaaatttg ataagattag ggatgcttac
     2401 ccgccagttg gtgaagaacc tgcaacagac gaattgtcat taatggatga attgccttta
     2461 acacccgtta aaacaattaa tttcacttat gagtattttt tatgtaaagg cgtacctact
     2521 aatcctgatc ctgcacataa tggacggtta actttaacta aagtggatta tacctattat
     2581 aactcaaaca aatctaaatt caattactat aagtttgagt ataatacagc ggattttccg
     2641 gatggtgatg atgctgatac tgatccagac ttcgaaccag catatcaccc acttgcttct
     2701 gataaatggg gcactatcca accctatgat cctgcgtttc cgacacaagt tttcccttat
     2761 accaaacaag ataatagagc attgtcagac tatttagctg gattgtggag cttaagaaga
     2821 attacattgc caaacggcgg aaccattgaa gttgactatg aagctgatga ttatgcttat
     2881 gtgcaaaata ggaaagctgc aaccgcgttt aaagttagat cctgtgcaaa aggttttgat
     2941 gaaattgggg atgacgtgat agttagtaat ttagaagaag ataacgctta cctatatggg
     3001 gccgatgtta ccggtgaaaa tttcctggta aataaataca tcatcacatt tgaacttcca
     3061 gaaccaattg aaggagacat gtctccaact gctgcaagaa attattttaa agacaattat
     3121 ttaaaagatt atagtggaag caaacacatt ggagataatt tatatttcaa attttatatg
     3181 ggtactgata acgatgatga tttgtttgtt gacgaaacac ctcatgacat agaatatcga
     3241 tttgaatatg tagaaggata tgcaagcgtt gattataata atgtaggtgt tttaaaaacg
     3301 atagataatg aagagacccc ttatacaatt gggtatttta aactaaaccc tgcaaaagtg
     3361 tatactgctg ctggcgattt tgcagcaagc gcttttaaac ctgaagatta tgacgatggt
     3421 ttctttggaa ctaaagatgt gaatccaata ataaaagcat cctggcagtg gaccatgttg
     3481 aatgcacaac aaaatattac agatgatagt ggaccagatg agtcactagg agcgcttgat
     3541 tatttatttg atttggttag taaactaaca atggtaaata ccttattgac tatgtttatg
     3601 aaccctaatc aattaatggc agatggtaaa tatggaaatg aatttgaccc ggaaaaatca
     3661 attattcggc tccatgaacc aagtggattt aaaataggtg gtgggcatcg agttaaagaa
     3721 ataagatact atgatgactg gcgtgatatg acagcagatg aaaaccctga aaaatcttcc
     3781 atttatggaa ttaaatatgt ttatcaaaat ccaaaaaatg attttagctc aggtgttgct
     3841 gcttatgagc caatggctgg tggcgaggaa aatccattgc gatacccaga cacctacaat
     3901 cattacgcag gagactttgc ctatgcacgt tatttggaac acccattagg cgaatcgttt
     3961 tatccatcgg cttctatcgg ttatgattat gtggaagaaa ttccttttgg tcagtttgaa
     4021 actgctgaag aaacatggag cgagcaatat actgatgttg gcaaaatagt gcgaacattt
     4081 tatacagcaa aagattatcc atcaattaca aaaaatacag aacttaatcc gataaataaa
     4141 aaatttaaac caggagtttt cgcatctttt tttcaatcta cgcattgcga cttattcgcg
     4201 ggttcacaag gttttgttat tgaaacaaat gatatgcatg gaaagccgag aacagaggca
     4261 accttatcaa aaaccggtga agtaatttca agtgttgaat ataaatataa agaatccgct
     4321 cccggagttt taaataatac ggtaaatgta attgatgaag ttggtgaagt cactccagca
     4381 gaaatgaacg taacatatga tgtatttaca gaccatagat taaataccgc tgctagttta
     4441 ggtggaggtg ctaaactcgg tttagatttt tccttgattg ttccagtagt tgaagcatat
     4501 ccaaactttg taggcactaa gtcaactacc gcattttcag tggttacaaa acatgtgcaa
     4561 cgaagtggta tcctaagtca ggttattcga aacaccaatg gcagtatttt aacttccaat
     4621 aaccttgtgt atgacaaaag gaccggtaat ccaattgtag taagcgaaca aaacgaattt
     4681 gaggatactt attacgtagt aaatattcca gcgcattgga tatatgatgg catgggtatg
     4741 gcaaacgata actgggattt caaatttgtt agccataata atgatgttac aacggacgat
     4801 gatttattta atttgtctac cggtcaaatt acatctgcaa ctttattaaa caac
//
LOCUS       DAORKW010000252         4836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_770_length_4836_cov_5.019661, whole genome shotgun sequence.
ACCESSION   DAORKW010000252 DAORKW010000000
VERSION     DAORKW010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_770_length_4836_cov_5.019661"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3876)
                     /locus_tag="PLB46_10260"
     CDS             complement(<1..3876)
                     /locus_tag="PLB46_10260"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HRF76945.1"
                     /translation="MSEKLQTKVELKEISIDVLDHLVLKGLYIEDQNRDTLIYAGKLE
                     VNIGTLNPFTPSIRLKNISLSETTINIKRTLPDSIANYSFIVDAFGGSSSTDTNSAPS
                     SGGKSVELRLNKINISTTTFRYYDEVAASDMFMYIDDSEILINNIGLEEHVLALEKVQ
                     FDATTFKLTGLLDTIPSTSEDSWDTIHIAMGDWTLETTQLLLNDCAFYFKDINDTIQT
                     TGINFSDMAVTNINIDMSDILYSGDTILNTINHLSLKEKSGFQVDTLRANVIFSPYEI
                     SLKGMLIKTPNSRIADAFGLSFSTLNSFDRFETEIRMDGNFNKSIISNKDIAYFAPEL
                     NAYEVLLELSGHVYGTLDNLKGRDFDAQVNNTAGIKGTLDIKGLPNIDETFLDLAFEP
                     LYVNTYELESIIGKGKLPESILKLGTINYNGRVTGFAYNLVTFGDIVTDEGNMYTDVN
                     FQYDPETKTSGFKGNLTTTALNVGKISGQSDILGKISMTASIDGFIGNDNTSDFDVET
                     KINAVEFYGYNYTNIEVDGQFQNDYFEGNFAIDDPNIEMTFNGIIDMQDSVPVYDFKS
                     KIERANLKKLNFYPEPLILSATASMNAEGTSLDNMIGDIHFGDLLLIRDKYIYRLDTL
                     NIVAKVDTGQKSLTANSNLFDFQIAGNYNLSTLPNAIKNMVNHYTNGNDTVVYAPQIA
                     KYELSIKNADKLMAIFYPDIQVVRNLKVSGDFNTGTNNFTTRLRVGQFNYDNILLDTL
                     LVEARTINDKLEFFTSLNSTTINNNITLPVLKTDGNFAQNKLSYNLKFGADTDSNRIN
                     LNGEVAFIDSLMAFNILPSEIYFQAEKWDILANNSLQYVNNNIIAQNFTLSSGTKVIS
                     LSSAPDPTYTTILKLNIRNIPIGDLVEQYVLQGESISGTLDATYSIGNVLGDPSFMGG
                     AEIKGLTLNNVLLGNLKLNTSLISPSNRLKFSSTLVGDNGFTTDGFYVLGEGEVEDSI
                     QLKAEFKKTQLMVVEPFLRGILSDMDGTINGALSVNGPASRPIMEGDLVVKKGGLTID
                     YLGTHYYFDQIDINIGRNKILIPETTVTDKLNNQGKLKGEITYSNFDAWNFRELSLKS
                     ENLILMETDAKQNPDLFGYAIGKVDANITGKLDALNIAVVTTPHSGTVVNLPTYGSGN
                     VKRHDFIRFVNKTDTTKLTTAPDELSLAIVDIDLKLNATPDAEIKLLINSEGTEYLTG
                     KGFGALNIKANSLGKVDMTGTYRITEGLYDFSFQGLFQRPFKVVPGSTIEFSGSPYNA
                     KLDLTAEYVAKDISVSSLTSTDTKEKTDVN"
     gene            4080..>4836
                     /gene="tsaD"
                     /locus_tag="PLB46_10265"
     CDS             4080..>4836
                     /gene="tsaD"
                     /locus_tag="PLB46_10265"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRF76946.1"
                     /translation="MPVTILGIESSCDETSAAVLIDGQIVANYIANQSIHEQYGGVVP
                     ELASRAHQANIVPVVDFALKTAGISLQQIDAVAFTQGPGLLGSLMVGAGYAKGIALAL
                     NIPLIAVHHMQAHILAHFIDDPKPDFPFLCLTVSGGHTQIVRVDSHLEMEIIGKTNDD
                     AAGEAFDKCAKMLGLPYPGGPLIDKMAVNGDPKAFAFAEPKIPGLDFSFSGFKTSVLY
                     FLQEQLKHDPNFVEHNLPDLCASIQHSIVEILLK"
BASE COUNT         1456 a          916 c          835 g         1629 t
ORIGIN      
        1 attcacatcg gttttttctt tcgtatcggt tgatgttaat gaacttacac taatgtcttt
       61 agcgacatac tctgcagtta aatctaattt cgcattgtag ggtgagccgc taaattctat
      121 ggtgctgccc ggaacaacct taaatggcct ttggaataaa ccttggaatg aaaaatcata
      181 taacccttcc gttattctgt aagttccggt catatctact ttgcctaatg aattcgcttt
      241 aatgtttaat gcaccaaatc ctttaccggt taaatattcc gttccttcgc tgttgattaa
      301 taattttatc tcggcatcag gagttgcatt gagctttaaa tcaatatcta caatagctaa
      361 gcttaactcg tccggagctg tagttaattt tgttgtatca gttttattta caaaacgaat
      421 aaaatcatga cgctttacgt tgcccgaacc ataggtaggt aaattaacta cagtacctga
      481 atgaggcgtt gtaacaacgg caatatttaa tgcatctaat ttacctgtga tgtttgcatc
      541 aactttacca atggcatatc cgaataaatc aggattttgt tttgcatcag tttccattaa
      601 gatgaggttc tcacttttta aacttaactc acggaaattc caagcatcaa agttggaata
      661 agtgatttca ccctttaatt tgccctggtt gttgagtttg tcagtaacag ttgtttccgg
      721 tatcaaaatt ttattgcgtc caatattaat atctatttgg tcgaaataat aatgggtgcc
      781 taaataatca atagtgaggc cacctttttt taccactaaa tctccttcca taattggtct
      841 gctggctgga ccatttacac tcaaagcacc attaattgtt ccatccatat ctgaaagtat
      901 tcctctcagg aatggctcaa ccaccataag ttgtgttttt ttgaattcgg cttttaattg
      961 tatactgtct tcaacttctc cttcccctaa cacataaaat ccgtcagtcg tgaaaccatt
     1021 atcacctact aaagtacttg aaaattttaa tctgttacta ggactaatta aagaggtatt
     1081 caattttaaa tttcccagca agacattatt taaggttaat cctttaattt cagctccgcc
     1141 cataaaactt ggatccccca atacgttgcc aatactataa gtagcatcta atgttcccga
     1201 tatactttca ccctgtaaaa catattgttc tacaagatca cctataggaa tgtttcggat
     1261 attgagtttt aaaattgtcg tataggttgg atcaggagcg ctgcttaaac taattacttt
     1321 tgttccacta ctcagtgtga aattttgtgc gatgatatta ttgtttacgt attgtaaaga
     1381 gttgttagct agaatatccc atttttcggc ctggaaatat atttctgaag gtaatatatt
     1441 aaatgccatc aacgaatcga taaaggcaac ttcaccattt aagttaatac gattagagtc
     1501 ggtgtcggca ccaaatttta agttataact caatttattt tgtgcgaaat ttccatcagt
     1561 ttttaataca ggcaaagtga tattattatt gatggttgtg ctgtttaaac tggtaaaaaa
     1621 ttccagtttg tcgttgatgg tgcgtgcttc aacaagcaga gtgtctaata atatattatc
     1681 ataattaaat tggcctacgc gcaaacgtgt ggtaaaattg tttgtgccgg tattgaaatc
     1741 gccgctaact tttaaattcc gcactacctg aatgtcaggg taaaaaatgg ccatcaactt
     1801 atcagcattt ttaatactga gttcgtattt cgcaatttga ggtgcataaa caactgtgtc
     1861 gttgccattg gtgtagtggt ttaccatatt ttttatggcg tttggcaacg tgctcagatt
     1921 gtaattcccc gcaatttgaa aatcaaataa atttgaattt gctgtaagcg atttttggcc
     1981 ggtatcaacc ttagccacaa tgtttaatgt atcgagtcgg tatatatatt tatcgcgaat
     2041 tagtaataag tctccgaaat gaatatcacc aatcatatta tccaatgagg tgccttcagc
     2101 attcatagaa gcagtagcac ttagtattaa tggctcagga taaaaattca acttttttaa
     2161 attcgctctt tcaattttcg atttgaaatc ataaacagga acactatcct gcatatctat
     2221 aataccatta aatgtcattt caatattcgg gtcgtcaatg gcaaagttgc cttcgaaata
     2281 gtcgttttga aactggccat caacctcaat attggtgtaa ttatacccgt aaaactcaac
     2341 agcatttatt tttgtttcca catcaaaatc agaggtatta tcattgccaa taaaaccatc
     2401 aatacttgct gtcatactga tttttcctaa aatatcagac tgccctgaaa tttttccaac
     2461 atttaaagcg gtggtcgtaa gattgccttt aaatccggat gttttagttt ccgggtcgta
     2521 ttggaaattc acatcggtat acatattacc ttcatcggta actatatcac caaaggttac
     2581 caggttataa gcaaatcctg taacacgccc gttataattg atggtaccta attttaaaat
     2641 actttcaggt aatttccctt ttccgattat tgattctaat tcataagtat ttacatacaa
     2701 tggttcaaag gctaaatcga gaaatgtttc atctatatta ggtaaacctt taatgtccaa
     2761 tgttccttta atacctgcag tattgtttac ctgtgcatca aaatctcttc cctttaaatt
     2821 atccaatgtg ccatacacat ggccactgag ttcgagtaat acttcgtatg catttaactc
     2881 aggagcaaaa taggcaatgt ctttattcga aataatactt ttattaaaat tgccatccat
     2941 tctgatttct gtttcaaacc tgtcgaagga atttaatgta ctaaacgaca atccaaaagc
     3001 atctgcaatt ctggaatttg gtgttttaat gagcatccct tttaaggaga tttcataagg
     3061 ggaaaaaata acatttgctc tcagcgtatc aacctggaat cctgattttt cttttaagga
     3121 taaatgattt attgtattta aaatagtatc accactatat aaaatatcgc tcatatcgat
     3181 attgatattg gtaacagcca tatcactaaa attgataccc gtggtttgaa tggtatcgtt
     3241 aatatcttta aaataaaatg cacaatcatt taataataac tgagtagttt ccaaagtcca
     3301 gtcgcccatg gcaatatgaa tggtatccca actatcttcc gaagtcgatg gtatagtatc
     3361 caataaaccc gtcagtttaa atgtggttgc atcaaactgc actttttcaa gtgcaagtac
     3421 atgttcttct aatcctatat tattaattaa aatttccgaa tcgtcgatat acataaacat
     3481 atcgcttgca gcaacttcgt cataatatcg gaaagtggta gtgctaatat tgattttatt
     3541 taagcgaagt tcgactgatt tgcctccact tgaaggagct gaattagtat ccgtggatga
     3601 ggagccacca aatgcatcga caataaaact ataattcgct atagaatcag gtaaggtacg
     3661 tttgatgtta atagtcgttt cactcaaact gatatttttc agcctgatac ttggcgtaaa
     3721 aggatttaaa gtcccaatat tcacctccag cttaccggca taaattaggg tatcccggtt
     3781 ttggtcttca atatataagc cttttaaaac gagatggtct aaaacatcta tgcttatttc
     3841 cttcagttcc accttggttt gcagtttttc gctcaaccat gttgttgctt tggaagctac
     3901 ataattttgg acagccggaa ttctaagtag gaaaaaaatc agtaaaaaca gacccaaaag
     3961 accccaaaat aagataagtg caatccttcg gatgaaggaa taaattctcc aatatgtgaa
     4021 tagttgctgc acctacgcct ttcctgctta tttttacggg gttaaattac gaatattgta
     4081 tgcctgttac aattcttgga attgagtcaa gctgtgatga aaccagtgca gctgtattga
     4141 ttgatggaca aattgttgcg aattacatcg caaatcagtc tattcatgaa caatacggtg
     4201 gtgttgttcc cgaactggca tctcgtgcac atcaagcaaa tattgttccc gttgttgatt
     4261 ttgcccttaa aactgcagga atttcgctcc aacaaattga tgctgtagca tttacgcaag
     4321 ggcccggcct cctgggttca ctgatggttg gcgccggtta tgctaaagga attgccctcg
     4381 cactaaacat tccacttatt gcagtccacc atatgcaagc tcatattctg gcccatttta
     4441 ttgatgaccc aaagccggat ttcccgtttt tatgtttgac ggtaagtggt ggacataccc
     4501 agatagtgcg ggttgacagc catttggaga tggaaataat tggaaaaacc aacgatgatg
     4561 ctgccgggga ggcatttgac aaatgtgcta aaatgttggg attaccctat ccgggtggtc
     4621 ccttgattga taaaatggca gttaatggcg acccgaaagc ttttgcattt gccgaaccta
     4681 aaattccggg attggatttt tcatttagcg gcttcaaaac ttctgtttta tattttttgc
     4741 aagaacagtt aaaacatgac cccaattttg tggaacacaa cttgcccgat ttatgtgcgt
     4801 caattcaaca cagtattgta gaaattttgc tcaaaa
//
LOCUS       DAORKW010000253         4836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_771_length_4836_cov_4.202259, whole genome shotgun sequence.
ACCESSION   DAORKW010000253 DAORKW010000000
VERSION     DAORKW010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_771_length_4836_cov_4.202259"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="PLB46_10270"
     CDS             complement(<1..169)
                     /locus_tag="PLB46_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL9 family ribosomal protein"
                     /protein_id="HRF76947.1"
                     /translation="MEVILIKDVDNLGDANELVKVRDGYGRNYLIPRGLAVIANEGNR
                     KMMFERQKGEVA"
     gene            complement(177..395)
                     /gene="rpsR"
                     /locus_tag="PLB46_10275"
     CDS             complement(177..395)
                     /gene="rpsR"
                     /locus_tag="PLB46_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRF76948.1"
                     /translation="MMRKKYCRFKKSGIKYIDYKNPEFLLKFVNEQGKILPRRLTGNS
                     LKYQRKVAQAIKRGRQIAILPYVTDLLK"
     gene            complement(445..837)
                     /gene="rpsF"
                     /locus_tag="PLB46_10280"
     CDS             complement(445..837)
                     /gene="rpsF"
                     /locus_tag="PLB46_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRF76949.1"
                     /translation="MNQYETVIIFTPVLSDDDVKRTEEAYKDFLRSNGGEVVAEEHWG
                     LKQLAYKIKKKSTGIYYVLEYKGPSDINAKLDIQFNRDENVLRFVTIRLDKYSIDYNE
                     RKRRGEIGRKAKAGANSQDKLTKATEEA"
     gene            966..1817
                     /locus_tag="PLB46_10285"
     CDS             966..1817
                     /locus_tag="PLB46_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76950.1"
                     /translation="MKQLLFKTTIISAFAIATLAGCKKENLDTDTTIALDNSIADGAF
                     QDMMGVVSEQATNEGLSGFTIGSIHAKVADDCPTVTFSEALGVFPNTMTLDFGTSCVG
                     YMGVERSGKIMATFTGFYKDAGTVITLTTDNYYVNGSKVEGTKTVINEGLTIEGHPWF
                     SVIVDGGLITLETGETISWNSSRMREWMSGYDTPELEDDVYGLNDGVAAEYAITGVNR
                     NGTPFTAHIAQTLIKPMDCKYITSGILEVTPEDMATRSLDFGDGDCDNKATLSVGEFS
                     AEITLLY"
     gene            complement(2250..3854)
                     /locus_tag="PLB46_10290"
     CDS             complement(2250..3854)
                     /locus_tag="PLB46_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76951.1"
                     /translation="MRIIKTFIALLLMQSAIQAQTVQDAKKAIESEFYFKAKKILIGL
                     NATAPTVETNYYLGNVYSITGNIDSAKIYYKKASELVENKNALIYVAMGKLNLLNNKP
                     AEAKENFESAIKVSKSKSAEIFYQIGEAYYKIDNNEAIKYFDIAYSTDASLVINLLAY
                     GDAYLASEKPSEAMTKYEQARTVNDNIAVTHLRIARIHSKTGNHKDAIAEYEKTVALD
                     PTIAVAWKELGEEYYLAGDYAKVKPCFDKYVELNAEDKAARIVPAVTCYQIGDYTCAI
                     EEASKIIADEPNNFIAWRILYWAHFDFGDSLRKIDAARSATLFADGYKAVQSYWNIPD
                     KKVIALDYPYSARLAVEVKDTTKAMFFYNMAVSNDTTATQEVYAEYAKYLYNTKKYAD
                     AIVAYNQLITKFDEGPLDVYFLGRSYFQTGDFVNADTTYANFIIMQPNSPDGYLQRAK
                     TKGRIDGDDIKGSALPFYLKYIEVAEKDIEKNKKNLIEAYLYCAYWYNSIEQNTDACV
                     YIMKTKAIDPENTFVKELEQVVKCTN"
     gene            complement(3874..>4836)
                     /locus_tag="PLB46_10295"
     CDS             complement(3874..>4836)
                     /locus_tag="PLB46_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76952.1"
                     /translation="GFIRLTRVIMRTTANKMKMNKYIVLLLSATLFAAGCGKSKSFDK
                     DVENLNKGTLSIYADESYKVLLDELIQSYENVYPESDITPTYGSDAMILDAMLKDKTR
                     MMVTGRVLSDNELAALEKVNGVAIKQFTIGKEAIAVITSQKNTDSIFNIDEFIASTKP
                     GYAGKYEKTGFVFNTKNTGMIKQLIGDAGGNYKNLFSLDNTDTLVAYIASNPEAIGFI
                     SFAEISDFDDPNAQQLLNGNKVLAVAKTDSTGKSIVTELSQSTIMTNKYPLLRSVNVV
                     KGNTSQLLGTGFVNFLYRSKASRIILKAGLIPEKMTERKINIVE"
BASE COUNT         1358 a          974 c          905 g         1599 t
ORIGIN      
        1 gcgcaacttc gcccttttgg cgttcaaaca tcattttacg gttgccttcg ttggcaatta
       61 cggctaaccc ccttgggatt aagtaattgc ggccataacc atcacgaact tttacaagtt
      121 cattggcatc gcctaaattg tctacgtctt taattaatat tacttccata gctcaattat
      181 tttaaaaggt cagttacgta aggtaaaata gcaatctgtc tgccgcgttt aatagcttgc
      241 gctactttgc gttggtattt cagactgtta cctgttaagc gacgaggtaa aattttacct
      301 tgttcgttaa caaatttcaa taagaactca ggattcttgt agtcgatata cttaattccg
      361 ctttttttga aacggcagta tttcttgcgc atcatcccta atttgggcgc tgcgaggtat
      421 ctgatgtttt ttccacctgc catattaagc ctcctcagtt gctttggtta acttatcttg
      481 tgaattagcg cccgctttgg ctttgcgtcc aatttcaccg cggcgcttgc gttcgttata
      541 gtcgatggaa tacttgtcta agcgaatggt tacaaagcgt aatacattct catcacggtt
      601 aaactggata tccaatttag cattgatgtc acttgggcct ttgtactcaa gcacataata
      661 aatacctgtg ctttttttct tgattttgta agctaactgc ttaaggcccc agtgctcttc
      721 ggcaactact tcacctccgt tcgaacgaag gaagtccttg taggcttcct cggtcctctt
      781 cacgtcgtca tctgagagga caggagtgaa aatgatgact gtttcgtact ggttcatttt
      841 tttgtttgtt tttagaaaat ggactgcaaa gatagccttt tttctttaaa atttacaaaa
      901 attccttaaa atgacgttaa accatttcct atcttagcgg tcaaagtccc aaaatcatat
      961 catttatgaa gcaattattg ttcaaaacaa ccatcatctc ggcgtttgcc attgcgacgc
     1021 ttgcgggatg taaaaaagaa aacctcgaca ctgacacaac tattgcttta gacaattcca
     1081 tcgccgatgg ggcatttcag gacatgatgg gtgtggtgag cgaacaagcc accaacgaag
     1141 gcctgtccgg gttcacaatt ggctctattc acgctaaagt tgccgatgat tgccctaccg
     1201 ttaccttcag cgaagcgcta ggcgttttcc cgaataccat gaccttagac ttcggcacga
     1261 gctgtgtcgg gtatatgggc gttgagcgtt cagggaaaat tatggccaca ttcacaggtt
     1321 tttacaagga tgcgggcact gttattactc ttaccaccga taattactac gtaaacggga
     1381 gcaaggtaga aggcaccaaa actgttatca acgaaggtct tactattgag gggcatcctt
     1441 ggttttcggt aattgtagat ggtggtctca ttaccctgga aacaggagaa acgatttcat
     1501 ggaatagttc ccgcatgcgc gaatggatgt caggatacga tacacctgaa ctggaagatg
     1561 atgtgtatgg cttaaacgat ggggttgcgg ctgaatatgc tattacgggt gttaaccgca
     1621 atggaactcc ttttacagcg catattgccc aaacattgat taaaccgatg gattgtaaat
     1681 acattacctc cggtatccta gaagttactc ctgaggatat ggccacccgc tcactagatt
     1741 ttggtgatgg tgattgtgat aataaagcaa ccttatcagt aggtgaattt tcggcggaga
     1801 ttacgctgtt gtattaattc acgtccaaac ataaaaccaa aacaagccgc ctgtatttac
     1861 gggcggtttt ttgttgcacg cggaggtgcg gagagggcgg agaaggattt atattaaaaa
     1921 ccattacata tttaattgga tatcttcctc cgcgtcttcg cgtgaaccag ataaaaaatg
     1981 tttctcgcta aggcccaaag gtttttttat atgttggttt aagtctaaat aaaaaaccaa
     2041 ttcaagcgcg caaaggtata atcggtttac ctgctttgcg cgcttgaaaa caatccatat
     2101 cgcacataat acattcattt aatcttagcg cctttgcgag aaacaaccgg ctcagcgaga
     2161 aacaaccggc accgcgcttt cagtccgctg aggtggatga aacaaaaaaa cctcccgaag
     2221 gaggtttata ttatttgatg tgttctttat taattcgtgc atttaacaac ctgttccaat
     2281 tctttcacaa aagtattttc ggggtcaata gctttggttt tcatgatata aacacaggca
     2341 tcagtatttt gctcaattga attataccag taagcacaat acaaataggc ttcaattaaa
     2401 ttctttttat ttttttcgat atctttttca gctacctcaa tatattttaa ataaaaaggt
     2461 aatgcacttc ctttaatatc atctccatct atacggcctt tagttttagc acgttgcaaa
     2521 taaccgtcag gtgaattagg ttgcataatt ataaagttcg catacgtagt atccgcattt
     2581 acaaaatctc cggtttgaaa ataagaacga ccaaggaaat aaacatcaag cggaccttca
     2641 tcgaatttag taatcaattg attgtaggct acaatggcgt cagcatattt tttagtgttg
     2701 tataaatact tcgcatattc agcatacact tcctgcgttg cagtagtatc gttagacact
     2761 gccatattgt aaaaaaacat agcttttgtg gtatctttta cttctacagc aagtcttgcg
     2821 gaataaggat aatccagggc aattactttt ttatccggaa tattccaata agattgcacc
     2881 gctttataac catctgcaaa taaagtagct gagcgagctg catctatttt gcgtaatgaa
     2941 tcaccaaagt cgaaatgcgc ccagtataaa attctccatg caataaagtt gttaggttca
     3001 tcagcaatta ttttacttgc ttcttcaatc gcacaggtat aatcgccaat ctgataacag
     3061 gtaactgcag gaacaatacg cgcagcttta tcttcagcat tcagttcaac atatttatca
     3121 aaacatggtt ttactttagc ataatcaccg gcaaggtaat attcttcacc aagttctttc
     3181 catgcaacag caatggtagg atctaatgct acagtttttt catattcggc aatagcatct
     3241 ttatggttac ctgttttaga gtgaatacgt gcaatacgca agtgagtaac agcaatatta
     3301 tcattcactg ttcttgcctg ctcatattta gtcattgctt cgctaggttt ttcggaagca
     3361 agataagcat caccataagc caataagtta attactaaac tggcatcggt actgtaagca
     3421 atgtcgaaat atttaatggc ttcattatta tcaattttat aataagcttc accaatctgg
     3481 taaaatattt cagcactttt tgatttgctt actttaatag cactttcaaa attttctttt
     3541 gcttcagcag gtttgttgtt gagtaaattt aatttaccca tagccacata aatgagggcg
     3601 tttttatttt caaccaattc gctggctttt ttgtaataga tttttgccga atcgatatta
     3661 ccggtaatac tgtaaacatt acctaaataa taatttgtct ccacggtagg tgcagtagca
     3721 tttaatccga taaggatttt tttagcttta aaataaaatt cggactctat cgctttttta
     3781 gcatcctgca cagtttgtgc ctgaatagca ctctgcatta acagtaaagc gatgaatgtt
     3841 ttaatgattc tcattttagg ttggtattta tttttattct actatattaa ttttcctttc
     3901 agtcatcttc tcaggaatta accctgcttt taaaatgatc cttgaggctt tgcttctata
     3961 taagaagttg acaaaacccg tgcctaataa ttgggaggtg tttcctttaa caacgttaac
     4021 acttctcaaa agcggatatt tattggtcat tatggtcgat tgtgacaatt cagttacaat
     4081 actttttcct gttgaatcgg ttttggctac tgctaatacc ttgttgccat tgagtaattg
     4141 ttgggcattt gggtcgtcga aatcactaat ttctgcaaac gaaataaacc caatagcttc
     4201 cggatttgac gcaatatacg ccacaagtgt atcggtatta tccaacgaaa acaaattttt
     4261 ataattgcca ccagcgtcgc cgattaattg tttaatcatg ccggtatttt tggtgttaaa
     4321 cacaaatccg gttttttcgt atttgccggc atatcccggt tttgtgctgg caataaactc
     4381 atcgatatta aaaatgctgt cggtgttttt ttgagatgta attacggcaa tggcttcttt
     4441 gccaatggta aattgtttaa tcgccactcc gtttactttt tctagcgcag ctaattcatt
     4501 atcgctcaat acacgacctg ttaccatcat gcgggtttta tctttcagca tggcatccag
     4561 tatcatagca tctgagccgt atgttggggt aatatcagat tcggggtaaa cattttcgta
     4621 cgactgaata agttcatcta acaacacttt ataactttca tcagcataaa tactcagtgt
     4681 gcctttgttc aggttttcta catccttatc aaaagattta cttttcccgc aaccggcagc
     4741 gaagagggtt gcagacagca ggagcactat gtatttattc attttcatct tgttcgctgt
     4801 tgtcctcatt attactcttg taagcctgat aaaccc
//
LOCUS       DAORKW010000254         4827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_773_length_4827_cov_4.777242, whole genome shotgun sequence.
ACCESSION   DAORKW010000254 DAORKW010000000
VERSION     DAORKW010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_773_length_4827_cov_4.777242"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(469..1152)
                     /locus_tag="PLB46_10300"
     CDS             complement(469..1152)
                     /locus_tag="PLB46_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76953.1"
                     /translation="MKKFQSLMFLLALGLGWLMFACDSSDPENISVTARSFRITHSGS
                     EFSVAIGTTDISDTGAVATSGGVRNINIENIHLPDSIDLKNIDANLADKKIVKDATQQ
                     LNKAPIGTTDISDTGAVHVRSVRDTTLIGATDISDMGGVLVLSDNTILAFTFNADGKA
                     LMESIDMMPSAAVKQTCFALTSNILVKNGEFIVKRSDGSVVSYNATTNIVILRKEGDE
                     LCVYLKPKS"
     gene            1266..2837
                     /locus_tag="PLB46_10305"
     CDS             1266..2837
                     /locus_tag="PLB46_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76954.1"
                     /translation="MPWKSIFMILLCKIPLNVMNAKDDLLSLIQSLNQTEKRYFKIYA
                     QRHVIGKSNAYIELFEAVETAGKKGDYNEIAIKQKLKKVIPASSYAVIKNYLYNLILK
                     SMRAYHEDDTTTHAISQRMLDIEFLMQKGLYEKALKMLNTTKQSARTLNRNFLLIEIL
                     SIQRRLMRHTLEKSSEATLQASSEEEMVCLQKITLELKYHHLYDQLFVMLQKYYQFRD
                     EKLLNKVQKIMKDPLITEPPQDLSFESVIMYNLIHSDHAHLTGDTHKSKLHRKKIIEA
                     YEEYPKMQADESLRYLNSLNNYLTSCFHSNDLDEVSKTLQKIKSIVPANLEEESQIFK
                     NSTYLELLFYIQTNQFVLAKKLVPVIESGLKKFGSKLPKGRILTIYYNIAVLFLVIEE
                     FDECLTWVNHIIFGEWENVRQDIQDSAKMLQIILHYELGNVDLLDSLMRSTRRQLKES
                     ERLYEMETLLLQLVKKLIRTPGKKERNQALEQVKQKISDLSNINGQVITGTEELLLWI
                     ESRLNGRSIADNMGK"
     gene            complement(2890..>4827)
                     /locus_tag="PLB46_10310"
     CDS             complement(2890..>4827)
                     /locus_tag="PLB46_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76955.1"
                     /translation="YHDGAGFIDAGMTNFKVANIADGTPATHPLQYMAYIYLDTTNHK
                     KYLGCSKAVIPTVKAILSWNTPVPNDPNYHPHYGNVIERNIQLKPLLIFKPFDPGDLV
                     FYPKPKFVDIPKLVDKAFPIPPLPPIEKLYKTYKSNQVPDHRMLYTSIAPMVMEKSFA
                     TTALKYQPNEIAQLGIDYDKLIEVILGNDDKADVAFEELVCVGLNSATDTLGAVIHVK
                     KNSGYSGDLCSTGSAEYVAFWADWDNNGSFDEYLGTAEVRVHDLDNIPTDGLYYNVAL
                     PIDVTKHLRACSKPNVVRIRAVLSWSSLPSTTNPNVLNHWGNRLDALVQVRPGRATDG
                     ELDVVITTVNEVSISDINTDGLAEPTAAFPNAMRPFGGEVMLSGYFTGSGAPGTVHYR
                     VEYSNNDGASWNPIMDKQRFIIEGSPFPTLDEQDASMTDGWLAYKGSTSPLQIIRFNV
                     LAIWDTGVKNGKHKLRVAFSDEPFAMPYNPVIINYSNEVAVHLDNDGFNVNPAPHAAG
                     DPLSSIHDIDIIIDGGECNFYKTGVDTLLTGRIKVMDDYFHSWNLDVQPSSHFIPPAT
                     LSTIVTPTGRACTGISDFGDNNFAFSIDLTKLDPCGYTVRIYGHDRAILGHRSYFIDG
                     TYSFNLGNHQGYKYAGFSILP"
BASE COUNT         1615 a          926 c          871 g         1415 t
ORIGIN      
        1 agaataactc ctgctataat ttaatccagc ccgtcagggc gacacaacgg tatctcagcc
       61 ctcccagtgc acacaccgca ccgacgagcc cgtcagggcg gcataacacc ggatgatatg
      121 acatagagca acccaatctg tcgccctgtc gggcttgatg tcatacgcta gttgggtatt
      181 actgatatat cgccctgtcg ggcttattgt tgagtttatg ttgcaaagaa taactcctgc
      241 tttaatttaa tccagcccgt cagggcggca caacggtaac ttagccctcc aagcggatac
      301 cccgcaccga cgagcccgct agggcggcat aacactggat gatatgcctt atcataaccc
      361 tattgctaca ctgatgggtt gaatgttgag cgtatatcac aaaaaaaccg tgcaaacacc
      421 acatttgcac ggttaccgat ttaacctgat gagttaaacc tcgtaaactt atgatttagg
      481 tttgaggtaa acgcagagtt catcgccttc ttttcgaagt atgacaatat tggttgtagc
      541 attgtagctc acaacagaac catcacttct ttttacaata aactcaccgt ttttaaccaa
      601 tatgtttgat gttaatgcaa aacaagtttg ttttaccgca gcacttggca tcatatcaat
      661 tgattccatc agcgctttgc catcagcatt aaatgtaaat gccaatatgg tattatcact
      721 caatactaac acgccaccca tatccgaaat atcggtagca ccaataaggg tagtatcacg
      781 aacagacctt acgtgaacag cacccgtatc cgaaatatca gtggtgccaa taggagcttt
      841 atttaattgt tgagtagcat cctttacaat ttttttatcc gccagattcg catcaatatt
      901 ttttaaatca attgaatctg gaaggtgaat attttcgata ttaatattac gcacaccgcc
      961 ggatgttgca actgcaccgg tatcagaaat atcggttgta cctatggcaa ctgaaaattc
     1021 actgccactg tgagtaattc gaaaagatct tgccgtaacg ctgatatttt ccgggtcgga
     1081 actatcgcag gcaaacatta accagcctaa tcctaaggct agtaaaaaca taagtgattg
     1141 aaactttttc ataattgatt ttttgggttc agaatgaata agaacaaagg taaaatatca
     1201 ctaaacatcc acctagatag gatttgcaca ttattaaatg tcaagtgaat aaaaattgtt
     1261 aaataatgcc ttggaaatca atatttatga tacttttgtg taaaatccca ttaaacgtca
     1321 tgaatgctaa agacgacttg ttatccctca ttcaaagttt aaatcaaact gagaagcgtt
     1381 attttaagat ttatgcgcag cgacatgtaa ttggcaaatc caatgcctat atcgaattat
     1441 ttgaagccgt agaaactgcc ggtaaaaaag gtgattataa tgagatcgcc attaaacaaa
     1501 aactcaaaaa agtaattccg gcaagttcat atgctgtaat aaaaaactat ctctataatt
     1561 tgatacttaa aagcatgcgg gcatatcacg aagatgatac cacaacccat gctataagtc
     1621 agcggatgtt agatatagaa tttcttatgc aaaaaggatt gtatgagaaa gcattaaaga
     1681 tgctcaacac gactaaacaa tcagcaagaa cactgaaccg caatttttta ttaatcgaaa
     1741 ttttatccat ccagcgcaga ttaatgcgac atactttgga aaaaagttct gaagccactt
     1801 tacaagcttc atctgaggag gagatggtat gtttacaaaa aataacctta gagttaaagt
     1861 atcatcattt gtacgatcag ttgtttgtga tgttgcaaaa gtattatcag ttccgtgatg
     1921 agaaattatt gaacaaagta caaaaaatca tgaaagaccc gctcattacg gaaccacctc
     1981 aggatctttc gtttgagtcg gtgattatgt ataatttaat tcactcagac catgcacatt
     2041 taacaggaga tacacataaa tcaaaactcc atcgcaaaaa aataattgaa gcctacgagg
     2101 agtatcctaa aatgcaagcc gatgaatcgt tgcgatattt aaattcgtta aataattacc
     2161 tgaccagttg ttttcattcc aacgatttag acgaagtatc aaaaacatta caaaaaatta
     2221 aatcaatagt cccggccaac ctggaagagg aatcgcagat ttttaaaaac agcacttatc
     2281 tggaattgtt attttatatc cagaccaatc agtttgtttt ggcaaaaaaa ctggttcctg
     2341 taattgaatc cgggttaaaa aagtttggca gcaaattacc caaaggaagg atattaacta
     2401 tttactataa tatcgctgtg ttatttttag ttattgaaga atttgatgag tgtttaacct
     2461 gggttaatca tatcattttc ggtgaatggg aaaatgttag gcaagacatt caggattcag
     2521 ctaaaatgtt acaaattatt ttgcactacg aattaggcaa cgtcgacttg ttagatagtt
     2581 tgatgcgctc tacacgcaga caattaaaag aaagtgaacg attatatgaa atggaaacat
     2641 tgttattaca attggtgaaa aaactcattc gcacaccggg aaaaaaggaa cgcaatcagg
     2701 ctttggaaca agtgaaacaa aaaattagtg atttaagtaa tatcaatgga caagtaatta
     2761 ccggtacgga agaattatta ttatggattg aaagccgtct aaacggaaga agtattgccg
     2821 acaatatggg taaataaaaa aacagccgga aaaaatccga ctgttttgat aacggtagta
     2881 attaatcaat taagggagta ttgaaaatcc cgcatattta tacccctgat gattacctaa
     2941 attaaatgaa taagtaccat caataaagta tgaacgatgt ccgagaattg cgcggtcgtg
     3001 accataaatt ctcacggtat aaccacatgg atccaatttt gttaaatcga tgctgaaagc
     3061 aaaattatta tctccaaaat cgctgattcc ggtgcatgct ctaccggttg gggtaacaat
     3121 agtagacaaa gttgcaggtg gaataaaatg tgatgaaggt tgcacatcta aattccagct
     3181 atggaaataa tcatccatta cttttattct acctgttaac aaagtatcaa cgccggtttt
     3241 ataaaaatta cattcaccac catcgataat aatatcaata tcgtgtatac tgcttaatgg
     3301 gtcgccagca gcatgtggtg caggatttac attaaatcca tcgttatcta aatgcacggc
     3361 aacttcatta ctgtaattaa taattacagg attataaggc atcgcaaatg gttcatctga
     3421 aaatgcaaca cgtaatttat gtttgccatt ttttacaccg gtatcccata tcgctaaaac
     3481 attaaaacga ataatttgta atggcgatgt actacctttg tatgcaagcc atccgtctgt
     3541 catacttgca tcctgttcat ctaaagtagg gaatggactt ccttcaataa taaatcgttg
     3601 tttgtccata atcggattcc aagatgcacc atcattgttg ctatattcaa cgcgatagtg
     3661 aacagtacct ggtgcaccac ttccggtaaa atatccgctc aacattactt caccgccaaa
     3721 tggacgcatt gcatttggaa aagcagcagt aggttcagct aaaccatctg tattaatatc
     3781 gctgatacta acttcattca cggtagtaat taccacatct aactcaccat cagttgctct
     3841 gcccggacgc acttgaacca atgcatctaa tctgttaccc caatgattta atacattcgg
     3901 attggtagtt gatgggagag aagaccatga aagtacggcg cggatacgca ctacatttgg
     3961 tttactgcag gcgcgtaaat gtttagtaac atcaattggt aatgccacat tgtaatataa
     4021 accatcagta ggaatattat ctaaatcgtg cacacgaact tcagctgttc ctaaatattc
     4081 atcaaatgaa ccattattat cccaatcggc ccaaaaagca acatattctg cagaaccggt
     4141 tgaacataaa tcaccggaat aacccgaatt ttttttcacg tgaataactg caccaagagt
     4201 atcagttgct gaatttaatc ccacacatac taattcttca aaagctacat cagctttatc
     4261 gtcattacct aaaattactt caatcaattt gtcgtaatcg atacccagtt gagcaatttc
     4321 gttaggttga tattttaatg cagtagttgc aaaagatttt tccattacca ttggtgcaat
     4381 agaagtatat aacatacgat gatcaggaac ctgatttgat ttatatgttt tatacaattt
     4441 ttctattggc ggcagaggtg gaattggaaa tgctttatcc accaatttcg ggatatcgac
     4501 aaattttggt tttggataaa acactaaatc acccggatca aatggtttga aaatcagcag
     4561 cggttttaat tgaatattac gttcaatcac attaccgtaa tgcggatgat aattcggatc
     4621 atttggcaca ggggtattcc aagataaaat tgcttttaca gttggaatta ctgctttgct
     4681 acaacctaaa tattttttgt gattggttgt atccaaataa atatacgcca tgtattgaag
     4741 cggatgtgta gccggggtgc catctgcaat gttggctact ttaaaattgg tcataccggc
     4801 atcaataaat ccggcaccat cgtggta
//
LOCUS       DAORKW010000255         4822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_778_length_4822_cov_4.421649, whole genome shotgun sequence.
ACCESSION   DAORKW010000255 DAORKW010000000
VERSION     DAORKW010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_778_length_4822_cov_4.421649"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLB46_10315"
     CDS             <1..585
                     /locus_tag="PLB46_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76956.1"
                     /translation="RTNPNVVLLKEGVVIDKWAWRDLPSFEAIKKEHFADRKPTELIP
                     ITNEMFGIGVPVAEKIAASTEPYNEFFLQDSTGNDVTAMVINDTANICMVILNELTAD
                     KLTADKWKAILPVMKGIDSTGNKLFVVSSGSFNILKLMKKASGLEFDLYTSDKDVLYK
                     IMEQNTGVIYIKQGKVVAKFPDDKLPADFSFLNK"
     gene            595..1671
                     /locus_tag="PLB46_10320"
     CDS             595..1671
                     /locus_tag="PLB46_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF76957.1"
                     /translation="MIKYILQRLVYGFFVLLGVVIIVFFLFQLTRINPERQIAGEKAD
                     KATIENIKKELGLDLPVWQQLGLYLNDISPLSWHNNSDTTSRFYLSSEKYQYFRVLNI
                     GHTSMVIKKPYLSRSYVSKRSVSEILSERIPNTLVLALTAMIFATIVGITLGVIAATR
                     PNSMLDRACTTGSVLGISFPSFFVGIILQLVFAYLLASVTGLNMTGDLYVTDGYTGEQ
                     YLELRNLILPAIALGVRPVAVITQLTRSAMIDVMHNDYIRTARAKGLNERVILFRHAL
                     KNALNPVVTSVSGWLASLLTGAFFIEAIFNYNGIGYETVNALKMFDYPVAMGAILYTA
                     VIFVVISIAVDIVYSIIDPRIRIS"
     gene            1673..2179
                     /locus_tag="PLB46_10325"
     CDS             1673..2179
                     /locus_tag="PLB46_10325"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRF76958.1"
                     /translation="MRIYLIGFMGCGKSTWGKKLAEKLDCAFIDIDKRIEAIEGGTVA
                     EVFKIKGEEYFRKLEADVLRSTVLEKNAVISCGGGTPCYFDNMEWMKQRGKTVYLNLS
                     PENLFGRLRTRKEKRPLIANLDDDQLRDFIFQKLSEREAFYLQADIKIDPAVAKPKDI
                     LDNIKGKK"
     gene            complement(2185..2700)
                     /locus_tag="PLB46_10330"
     CDS             complement(2185..2700)
                     /locus_tag="PLB46_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRF76959.1"
                     /translation="MESTLTKIADAEKVYQKIKRIAFEIAEDNYDETEIVLAGICKAT
                     EGYVFAELLYKELSKISKAHILLTHIELDKNDPGNSVVELNLTEEEIDNKIIIVVDDV
                     ANTGRTILYAIKPLLSYSPKKIRNAVLVERKHKLFPVSPDYVGLSLSTTIHEHIKVIL
                     SEPGNEGIYLM"
     gene            complement(2703..>4822)
                     /locus_tag="PLB46_10335"
     CDS             complement(2703..>4822)
                     /locus_tag="PLB46_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF76960.1"
                     /translation="VMVIHNNVDSAYLGMWPLEQGYDGTGVVVGVIDAPFDIAHGDFN
                     DANGDSRIKYVWDQNLTDGTAPSPYTYGIECDSAMIADGLCPSTDDYELNYSHGTGVA
                     GIAASSGLAANRYRGVAPNADLILVSMNFETEFNTTITDAIAYIYERANTLGKPCVIN
                     TSVGLYDGSHDGTDLTAQLIDAMITEQNGRALVAAAGNAGSFPFHLGYDVTATEQFTW
                     FKKLSYAGVAYFQLYADEAAFNTVNFRISCDVPGTWISKGSTPMYNMLADFNFDTDII
                     DSVIYTIPGAGDVAVYAQLLNGQYFLEFLITPAVGTDYWRFSTSGSGRFDMWNAEATT
                     GFSNYVTTGLPTAAVLPEIVNYRGPDTDQSIVSSWQCLDNVITVGSYVNRDTMTNFYN
                     VIPPILDVVGALYISSSHGPTRDNRIKPDICAPGARVLSTASSILTNWLIEEGAANYM
                     SQDGQHYLYNGTSFSSPAVAGIAALYLQKYPNATAAEVRDAITSQAKSDAFTGASLPN
                     NLWGYGKADAFRTLTGSWGCGPDDYLDAPENLTADAFTATKIMLSWDLIPNAAGYQIY
                     YKPVAGVAQKAKAFSNTKTLSGLTPNTTYSIKVRAFCEGQGFSNWSETITVTTAPFKS
                     AAEEHQLIQIFPNPAANTFTVEGVSEGGYITMVNALGEMVYSTIVTDAVIRINTTTLQ
                     NGIYQLILEDKGSISTQKIMIVH"
BASE COUNT         1448 a          910 c          966 g         1498 t
ORIGIN      
        1 agaactaatc cgaatgttgt tttattaaaa gaaggtgttg tcatcgataa atgggcatgg
       61 cgcgatttac cttcatttga agcgattaaa aaggaacatt ttgcagaccg aaaaccaact
      121 gaattaattc cgattaccaa cgaaatgttt ggcattggtg ttccggttgc ggagaaaatt
      181 gcggccagca ctgaaccata taatgagttt ttcctgcagg acagcacagg gaatgatgtt
      241 acagcaatgg taattaatga tacggctaat atctgtatgg taattctgaa tgaattaaca
      301 gcggataaac ttactgcaga taaatggaaa gcaattttgc cggtaatgaa aggcattgac
      361 tcaacaggca ataaattatt tgttgtaagc agtggttcgt tcaatattct gaaattaatg
      421 aaaaaagctt cgggactcga atttgatttg tatacttcag ataaggacgt attatataaa
      481 ataatggaac aaaacacagg cgtaatttat atcaaacaag gtaaagtagt tgccaagttt
      541 ccggatgata aattacctgc ggacttttca ttcttaaata aataattttt cattttgata
      601 aaatacatat tacaacgtct cgtttacggt ttcttcgttt tactaggcgt tgtaatcatt
      661 gtattttttt tatttcaatt aacgcgcatc aaccctgaac gacaaattgc aggcgaaaaa
      721 gcggacaagg ctaccattga aaatattaaa aaggagctgg gtttagattt acctgtttgg
      781 cagcaattgg gcttatacct caacgatatt tctccactga gctggcataa taattccgac
      841 acaacttcac gattttattt aagcagcgaa aaatatcaat atttccgtgt gcttaatatc
      901 ggccatactt cgatggttat taaaaaaccg tatttaagtc gttcgtatgt gagcaaacgc
      961 agtgtgagtg aaatattatc agaacgtatt cccaatactt tagtattagc attaacagca
     1021 atgatttttg cgaccatagt gggtattaca ctaggtgtta ttgcggctac acgtcctaat
     1081 tccatgttgg acagagcttg cacaacaggt tctgtgttag gcatttcgtt tccatcgttt
     1141 tttgttggta ttattttgca attggtattt gcttatttat tagcttcggt aacgggatta
     1201 aatatgacag gcgatttgta tgtaactgac ggatacaccg gagaacaata tctggaattg
     1261 cgcaatttaa ttttaccggc aattgcatta ggtgtcagac ccgtggcagt aattacacaa
     1321 ttaacacgaa gcgcaatgat tgatgtaatg cacaacgatt atatccgcac tgcgagagca
     1381 aagggtttaa atgaacgcgt gatattattt cgccacgcgc ttaaaaatgc gttaaaccct
     1441 gttgttactt cagtatcagg atggttggcg agtttattaa cgggcgcttt ttttattgaa
     1501 gccattttta attataatgg cattggctac gaaactgtga atgcattaaa aatgtttgat
     1561 tatccggtgg caatgggcgc catattatat actgctgtta tatttgtagt gattagtatt
     1621 gctgtagata tagtttattc aattatagat ccacgaatta gaattagtta atatgcgcat
     1681 ttatttaatt ggctttatgg gttgtggtaa atccacctgg ggtaaaaaac ttgcagaaaa
     1741 gctggattgt gcatttattg atatcgacaa acgtatcgaa gccattgaag gtggaacagt
     1801 ggcggaagtt tttaaaataa aaggggagga gtatttccgc aaattagaag cggatgtatt
     1861 gaggtctact gttttagaaa aaaatgcagt catcagttgc ggtggaggta caccttgtta
     1921 tttcgataat atggagtgga tgaaacaacg cggtaaaaca gtgtatttaa atctttcccc
     1981 agaaaattta ttcggtcgct tgcgcacacg caaagaaaaa cgtccgctca ttgctaattt
     2041 ggatgatgat caactaaggg attttatttt tcaaaaatta tctgagcgtg aagcatttta
     2101 tttgcaggca gatattaaaa tagatcctgc tgttgccaaa cccaaagata ttttagataa
     2161 tatcaagggg aaaaaatgat gtaattacat caaataaata ccttcatttc caggctcact
     2221 taaaattact ttgatatgct cgtgtatggt agtagaaagc gagaggccaa cataatcggg
     2281 acttaccgga aataatttgt gtttgcgttc aactaaaacg gcattgcgta ttttttttgg
     2341 agaataactc agcaatggtt ttatggcata caaaatggtg cggcctgtat tcgcaacatc
     2401 atctacaaca ataataattt tattgtcaat ttcttcttcc gttaaattga gttcaacaac
     2461 actatttccc ggatcgtttt tatcgagttc gatatgcgtt aataagatat gtgctttaga
     2521 aattttggac aattctttat ataacaactc ggcaaataca taaccttcag ttgctttgca
     2581 aatacctgcc aatactattt ctgtctcgtc gtaattatct tctgctattt caaatgcaat
     2641 gcgtttaatt ttttgatata ccttttctgc atctgcaatt ttggttagtg ttgactccat
     2701 aattaatgaa ctatcattat tttttgtgtt gaaatactgc ctttgtcttc taaaattaat
     2761 tggtaaatac cgttttgtaa tgttgttgta tttattcgaa ttacagcatc agtgactata
     2821 gtggaataaa ccatttcacc taaggcattc accattgtga tataaccgcc ttcgctcaca
     2881 ccttcaacag taaaagtatt ggcagcagga tttgggaaaa tttgaattaa ctgatgttct
     2941 tctgctgcag atttaaatgg tgctgttgta acggtgatcg tttcgctcca attgctaaat
     3001 ccctgtcctt cacaaaaggc tctcactttt atcgaatagg tggtattagg tgttaatccg
     3061 cttaatgttt tggtattgga aaaagctttg gctttttgtg ctacccctgc aaccggtttg
     3121 tagtaaattt gataacctgc tgcatttgga attaaatccc aactcaacat aatttttgtt
     3181 gcggtaaatg catcagcagt taaattttcc ggtgcgtcta aataatcatc aggaccgcat
     3241 ccccagcttc cggttaacgt tctaaatgca tccgcttttc cgtatcccca taaattgttt
     3301 ggtaacgagg ctccggtaaa ggcatcactt tttgcctgac tggtaatggc gtcgcgcact
     3361 tcagctgccg tcgcgttcgg gtatttttgt aaatacaagg cggcaatgcc tgccacggca
     3421 ggtgatgaaa atgaagtacc gttgtataaa tagtgttggc cgtcctgtga catataatta
     3481 gccgctcctt cttcaattaa ccaattggtt aaaatagatg aagcagttga taaaactctg
     3541 gctcctggtg cgcagatatc cggttttata cggttatcac gtgttggtcc gtgactgctg
     3601 gaaatgtata aagcacccac aacatccaga attggtggga ttacattata aaagttggtc
     3661 atggtgtcgc gattaacata actacctaca gtaataacgt tatccaaaca ctgccagctg
     3721 ctgacaatgg actgatcggt atccggcccg cggtaattta cgatttcggg taaaacggct
     3781 gcagttggca atcctgtcgt aacataattc gaaaaacctg tagttgcttc agcattccac
     3841 atgtcgaaac gtccgctgcc actggttgaa aaccgccagt agtcagtacc cacagctgga
     3901 gtaattaaaa attcaagaaa atactgaccg tttaacaatt gtgcataaac agccacatct
     3961 cccgctccgg gaatggtata aataacagaa tcaatgatat cggtatcaaa attaaaatcg
     4021 gctaacatat tatacatcgg agtgctgcct ttcgaaatcc atgtgcccgg cacatcacag
     4081 ctaattctga agttaaccgt gttaaaagcg gcttcgtccg cgtacagctg gaaatacgct
     4141 actccggcat aactcaattt tttgaaccat gtaaattgtt cggtagcagt gacatcatat
     4201 cccaaatgga aaggaaaact accggcattt cctgcagctg ctactagagc acgtccgttt
     4261 tgttcggtta tcatagcatc aatcagctgt gctgtcaggt ctgtcccgtc gtgtgaacca
     4321 tcgtataaac ctactgaagt attaattaca caaggtttgc caagtgtatt ggccctttca
     4381 taaatatagg caatagcatc cgtaattgtg gtgttgaatt cagtctcaaa attcatggaa
     4441 accaatatca aatcagcatt tggagcaaca ccacgatagc ggttagcagc taatcctgaa
     4501 gaagcagcaa taccggccac accggttccg tgactatagt ttaattcata atcatctgtg
     4561 ctggggcaaa ggccatcggc aatcatagcg ctatcgcatt caataccata ggtatatggc
     4621 gaaggagccg ttccgtcggt caggttttgg tcccaaacgt atttaattct ggaatcccca
     4681 ttagcatcat taaaatcgcc atgcgcaata tcgaaagggg catcaatcac acccaccaca
     4741 acaccagtgc catcgtaacc ctgttcaagc ggccacatgc ccaaatacgc tgagtcgaca
     4801 ttattatgaa ttaccatcac at
//
LOCUS       DAORKW010000256         4808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_783_length_4808_cov_3.438250, whole genome shotgun sequence.
ACCESSION   DAORKW010000256 DAORKW010000000
VERSION     DAORKW010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_783_length_4808_cov_3.438250"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4069)
                     /locus_tag="PLB46_10340"
     CDS             complement(<1..4069)
                     /locus_tag="PLB46_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76961.1"
                     /translation="MDRLLTVNWQKSVNYYGKVIAILLLLNTHAIYGQYFEQSSAELN
                     LKNVNYASAATTTKQYTETEGFGGVGGFTDASVATYVQVYFDQAMNTYIPTDLVVNVG
                     LEIKRYPSVGAPIIETDTLRVSYSSDNLETYQDKAIKIWTDPVYYKIRAEIKFIKKDD
                     GTNITPSTLPINLKMFVGIRCERYYDFDKTDQPLATKIEYLDEIDAYSISGFDVPGAL
                     SYDVEWTYVDDYDNGGLSFALTPEETFFSFKNNATRINVAPGSKVILPAIFDRGYLIV
                     RARGIGPAPSDVTKLLLGAWSLEDAYGEGLGLFTKKTVDEIADADYYIHIEESVVATD
                     PTPHIPDMGWMVQTTFAEEGKMKHIVQYFDATGRVRQTATMANTEKEALVTETIYDYQ
                     GRAVFQSLPAPAPLVTTPAEGYKSKLKYYSGINQTTTADIYDKSAFTFNPELCMTTVP
                     ELGTGSGAGNYYSPDNGKKNNYHGFIPDANGYPFTFTGYKADNTSRVYKQSGVGETHR
                     MGGGHEVVTYYGTPDQVELDRLFGANVGLASHYFKTAIVDPNGQVSVSYTDIDGHVIA
                     TALAGIGADNVEPLMDGEATVAEASEEEVTVDVLNISLDEPNGEFNDLFTEGTSTGFL
                     VKDVFIVPEETPYNFVYTAEGVSYMGDCLTTHCFECVYDIEITLTDGCGNQYLNTVDG
                     NPFTIRIGQMPVLGEAPATVCDTPYYASNIADGEIFDVILPQGEYTLLKTITLNEEAM
                     HIHALNYIQQLEADNPICYETLTEVFDDANGLTFDCELDCSYCEEIDVFTDFLATKFE
                     SEAEYPAAESPEYDFYLGVYNDLKEECALICETNPFDPCEAGLMGMLADMSPGGQYGE
                     YINSATGLPDAAEYYLSVYNEDNFLQERNVLTGDAVASTYKNPKFYDLATSTWIDGYY
                     LDGVRTVIDLSYYEDMGILWSDVADSYTLDDLDGDGLNEKVCYPEKLKLPYFVSIYNY
                     EWGYSLICYHPEYYYYQNCVLNRDCVVNIENPETAVNEPQNTYSFEEILYTIDIADVE
                     ALPTAEEELWKQAQKLDPIFAATGCGTELTNLAYFEANYLLNFQHCESGEIIDIYEAV
                     YNSVNGIPGVGEPCEAMEQLAGADLEDHFYVINSDLAWALYVNFYRSAKQKYLNAVAH
                     YYAQGDVSDEDGEPDRFGINLSIGNDEFVAPLVYTYLSLDVDEYWYKLATNVSYQPFI
                     VRSILLQDKLVRFPDVSWHSGVPPTAAIDGPEITELVELGTASTDAAYYSLTGNKPLA
                     FDMETFLQGVLNHAEITVTDWRTADYPIEDIAGISERLYDALTERCLNEFDVTISTSV
                     ITNDLLTWTQETGCTPTEICTDNFLSLPATSAYACT"
     gene            complement(4086..>4808)
                     /locus_tag="PLB46_10345"
     CDS             complement(4086..>4808)
                     /locus_tag="PLB46_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76962.1"
                     /translation="LDIIHQTAINMKKYTIIICIMFCYAAVSAQTQDMQVEALFKNFA
                     SLDSYSLRYDVKTYLEKNKIAATDSFSMFYVKTGDKYAALERNVLVIGDPEYTVMVDD
                     NAQIIYVQTNSDDAYQSVNFFDAEQPVIKNASIADTIVNGVPAYVVNSFGDGYTSIRF
                     LFQKNNLMPFQIVMTAEPRFDELNGKQTIIFPKSVMNITYSNQPDFTGFKAVELQDVI
                     NFSSANQFSLTTKFSSYELYVL"
BASE COUNT         1541 a          975 c          760 g         1532 t
ORIGIN      
        1 ccgtgcaagc atatgccgag gtagcaggta atgaaagaaa attatcggta caaatttcag
       61 ttggtgtaca accggtttct tgagtccagg ttaataaatc gtttgttatt acagaagtac
      121 taattgtaac atcaaattca ttcaagcaac gttcagttaa ggcatcatat aaacgttcag
      181 aaataccagc aatatcttca attggataat ctgcagtacg ccaatctgtt accgtaattt
      241 cagcatgatt tagtacccct tgcaaaaatg tttccatatc aaaagcaaga ggtttgttgc
      301 ctgttaagga ataataagca gcatctgtgg atgctgttcc cagctccact aattcagtaa
      361 tttcagggcc atcgattgca gcagttggtg gcacacctga atgccaagac acatcaggaa
      421 aacgaacaag tttatcttgt aataaaatgg accttactat aaatggttgg taagatacat
      481 ttgttgcaag tttgtaccaa tattcatcaa catccaaact aagatatgta taaacaagtg
      541 gagccacaaa ttcatcatta ccaatggaca aattgatacc aaatctgtcc ggttctccat
      601 cttcatcact tacatcacct tgagcataat aatgtgcaac tgcattcaaa tatttttgtt
      661 ttgcacttct ataaaaattt acatataaag cccatgctaa atcagaattg attacataaa
      721 aatggtcttc taaatcggca cccgctaatt gttccatagc ctcacatggt tcaccaacgc
      781 cgggaatgcc gtttacagaa ttgtaaactg cttcataaat atctataatt tcaccactct
      841 cgcaatgttg gaaattgagt aggtaatttg cttcaaaata tgcaaggttt gttaattctg
      901 ttccacatcc tgtggcagca aatatagggt ctagtttttg agcttgcttc caaagttctt
      961 cttcagcagt aggcaaagct tcgacatcag ctatatcaat tgtatataat atttcttcaa
     1021 acgagtaggt gttttgcggt tcatttactg ccgtttcagg attctcaata ttaacaacac
     1081 aatccctgtt taatacacaa ttttgataat aataatattc cggatggtag caaataagtg
     1141 aatagcccca ttcataatta tatatactaa caaaataggg caattttagt ttttccgggt
     1201 aacaaacttt ttcatttaat ccatcgccat ctaaatcatc aagtgtatag gagtcagcaa
     1261 cgtctgacca taagataccc atatcttcat aataactcaa atctattact gtgcgcacgc
     1321 catctaaata ataaccatca atccacgtag atgttgctaa atcataaaat tttggatttt
     1381 tataagttga agcaactgca tcaccggtta aaacattacg ttcctgtaaa aaattgtctt
     1441 cattatatac agataaataa tattctgctg catccggcaa ccctgttgct gaattaatat
     1501 attcgccata ctgtccgccg ggactcatat cagcaagcat acccattaat ccagcctcac
     1561 aagggtcaaa aggattggtt tcgcaaatga gcgcacattc ttcttttaaa tcgttgtaaa
     1621 cgccgagata aaaatcatat tccggacttt ctgcagcggg atattcagct tcagattcaa
     1681 atttggtagc taaaaaatca gtaaatacat ctatttcttc acaataacta caatccaatt
     1741 cgcaatcaaa agttaaacca tttgcatcat caaaaacttc agtcaaagtc tcataacaaa
     1801 tcggattatc agcttccagc tgttggatat aatttaatgc atgaatatgc attgcttctt
     1861 cattaagtgt aattgtttta agcaaggtat attcaccttg tggaagaatt acgtcaaata
     1921 tttctccatc agcaatattg gaagcataat agggtgtgtc acatacagtt gccggtgcct
     1981 cacctaatac cggcatttgt ccgatacgaa ttgtgaaagg attaccatca accgtattca
     2041 aatattgatt tccgcaacca tcagtaaggg ttatttcgat atcgtaaaca cattcaaaac
     2101 aatgggttgt taaacaatca cccatataac ttactccttc tgcggtataa acaaaattat
     2161 aaggcgtttc ttccggcacg ataaatacat ctttcactaa aaatcctgtt gaagtgccct
     2221 cagtaaataa atcattaaat tcaccattgg gttcatctaa tgaaatattt aaaacatcca
     2281 ctgtcacttc ttcttccgaa gcttcagcaa ctgtggcttc cccatccatc aaaggttcaa
     2341 cattatctgc accgattcca gccaatgcag ttgcaattac atgaccatca atatcagtat
     2401 aagatacact tacctgtccg ttagggtcca caattgccgt tttaaaataa tgactggcta
     2461 atccaacatt cgcaccaaat aatcggtcta actcaacctg atccggtgtt ccgtaatagg
     2521 tcacaacttc gtgtccccca cccattctat gggtttctcc tactccactt tgtttataaa
     2581 ccctactagt gttatcagct ttataaccgg tgaatgtaaa tggataccca tttgcatccg
     2641 gaataaagcc atggtaatta ttttttttac cattatcagg tgaataataa tttcctgctc
     2701 cacttccggt acccaattca ggcaccgtag tcatgcatag ttcaggatta aaagtaaaag
     2761 ctgatttatc ataaatatca gctgtagtag tttgattaat acctgaataa tattttaatt
     2821 tacttttata cccttctgca ggtgttgtta ccaaaggagc aggtgcaggc aacgattgaa
     2881 aaacagcacg tccctgatag tcataaattg tttctgttac cagcgcttct ttttctgtat
     2941 tcgccatagt ggctgtttgc cttacgcgac cagtggcatc aaaatattgt acaatatgtt
     3001 tcattttgcc ttcctcagca aaagttgttt gcaccatcca acccatgtct ggtatatgag
     3061 gtgttgggtc agtagctact acactttcct caatatggat ataataatct gcatcagcaa
     3121 tttcatcaac agttttttta gtgaataatc ctaggccctc accatatgca tcttctaggc
     3181 tccaagctcc caacaataat ttggtaacat ccgaaggagc tggtccaatt cccctagccc
     3241 tgacaattaa ataaccgcgg tcgaatattg ccggtaaaat tacttttgac ccgggtgcaa
     3301 catttatgcg agtagcatta tttttaaatg aaaaaaaggt ttcttcagga gttaatgcaa
     3361 aagacaaacc accattatcg taatcatcaa cataggtcca ctccacatca taggacaatg
     3421 caccgggtac atcgaatccg gatattgaat aggcatcaat ttcatccaga tattcaattt
     3481 tagtcgctaa aggttgatca gttttatcaa aatcataata ccgctcacaa cggataccta
     3541 caaacatttt taaattaata ggcaacgttg aaggggtaat attagtacca tcatcttttt
     3601 taatgaattt aatttcagca cgaattttat aatagactgg gtcagtccaa attttaattg
     3661 ccttatcctg ataagtttcc aggttatcac tactatagga aactcttaag gtatctgttt
     3721 ctataatcgg tgctcctaca gaagggtaac gtttaatttc taatcccaca ttcactacca
     3781 aatcggtagg aatataagta ttcatcgcct ggtcaaaata tacttgcaca taggttgcaa
     3841 cagatgcatc agtgaaacca cctactccac caaaaccttc agtttctgta tattgtttag
     3901 tggtagtagc cgcacttgcg tagttgacat tttttaaatt taactccgct gatgattgtt
     3961 caaaatattg tccataaatg gcatgtgtat tgagtaataa aagaattgct attaccttac
     4021 cataataatt tacagatttt tgccaattca cagttagcaa tctatccatg gtctgtttgt
     4081 ttgttttaca acacatacaa ttcataggaa gaaaatttag tagtcaatga aaattgatta
     4141 gccgaagaaa aattaattac atcctgtaat tcaacagctt taaaaccggt aaagtcaggt
     4201 tggttagaat aggttatatt cattactgac ttaggaaaaa ttattgtttg tttaccattt
     4261 aactcatcga acctcggttc ggcagtcata acaatttgaa atggcattaa attgtttttt
     4321 tgaaatagaa atcgaatgga agtatatcca tcgccaaaac tatttaccac ataagcaggc
     4381 acaccattaa ctatcgtatc tgcaattgat gcatttttta taacaggttg ttctgcatca
     4441 aaaaaattga cactttgata agcatcatct gaattagttt gaacatatat aatttgggca
     4501 ttatcatcca ccataacagt atactcaggg tcaccgatta ccaaaacatt tcgttcaagt
     4561 gccgcatatt tgtcacccgt ttttacataa aacatagaaa agctatccgt tgctgcaatt
     4621 ttgttttttt ccaagtacgt ttttacgtca tacctcaacg aatacgaatc aagtgatgca
     4681 aaatttttaa acagtgcttc tacttgcata tcttgtgttt gagcactaac tgcagcgtaa
     4741 caaaacatga tacaaattat tatggtatac ttcttcatat taatcgcggt ttggtgtatt
     4801 atatctag
//
LOCUS       DAORKW010000257         4791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_787_length_4791_cov_4.311233, whole genome shotgun sequence.
ACCESSION   DAORKW010000257 DAORKW010000000
VERSION     DAORKW010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_787_length_4791_cov_4.311233"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1647)
                     /locus_tag="PLB46_10350"
     CDS             complement(<1..1647)
                     /locus_tag="PLB46_10350"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRF76963.1"
                     /translation="MNKFTPSLRRLIIAGGIMLSSATAFSQYCIPAYTTGTAEGDFID
                     GVTVSDLSNIGSGGAITDVGYSDYTGLTANLTIGTTYTMTLNNNPSFSQTYTAWIDGN
                     HDDVFSTDEVLGSVSLVAGATGSFDFSLAPGTMEGATRMRVRCIYPAFLATPLDPCAS
                     TSYGEAEDYTVVIEGAAAIDGGVVDVTAPTSGVTLGVESVTVTIQNYGSESLSGFNVS
                     YNVDGGAAVTELYAGTIAAYSTASYTFSTTHDFTADGCYEIGAWTSVDGDAAEINDDY
                     TETVCNLGPITGTGAVYMYSNTTGGEPWFTTTNTTAMNTVFGAEGIGWTREYFETADV
                     YAVFNPDNCFVFLEGSDSHAAELESFLATYGADIENWVASGGKLLLNAAPNEGDGMSF
                     GFDGTTLTYPYFTDNATAVDAAHPIFNGPFAPAGTDYTGGSFGHAIVSGTGYTNVMVD
                     LFSPSSAVLAEKPWGDGRVMVGGMTTNNFHSPLENAANLRANILAYLSCVEILFCETP
                     GGLFVDGITTNDAVCHWNAIDGADQYRFALPNTFTGVIAKR"
     gene            complement(1981..3534)
                     /locus_tag="PLB46_10355"
     CDS             complement(1981..3534)
                     /locus_tag="PLB46_10355"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF76964.1"
                     /translation="MSRKIFFNAKVLTQDKLMPEADAFIVEEDKFIAVGDTSTILAQQ
                     SADDILIDLEGNYVIPGFNDAHIHIWKVGNLMTHMLDLRTVRSIAEMQEKILDYANQH
                     PQLTWIQARGFNEANFIDKRFPTKYDLDLIPTDKPVCVTRTCAHQIIVNSKALAVAEI
                     NADTPVPSGGEMKLLPDGTVSGHFTETAIGLILSKIPRYTKSELRTMILAAQRYLLQL
                     GITAATDPAVDRDLLAVYKEMDANGELVLRINAIPIRVPDGANKVYPNPEHYQSEHLV
                     VNTVKFFADGGLSGKTAALLRPYKGNAGFGVLRLDAATFKSLAAESQQAGFKIATHAI
                     GDAAIKMVLDVYTSIAAENTQKLCHRIEHLGFPDNSDLQLMHQLGVIAVMQPVFIEEL
                     GNNFIDNIDEQYLSTVYPVRNVIEAGVTVAFSTDAPVVRQINPLNNIQSAISRKTLQG
                     KVIAENQRVTFKEALYAYTMGSAIAGNQANQLGSVEQGKFADFLILKHLGTNNAELVQ
                     TYIGGAPVV"
     gene            complement(3538..>4791)
                     /locus_tag="PLB46_10360"
     CDS             complement(3538..>4791)
                     /locus_tag="PLB46_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRF76965.1"
                     /translation="KSTEVAIAHAEGGVVTDVDGNVLLDFAGGIGMMNIGHSNKQVVD
                     AMKTQLDKYIHICSLVATPEPYVELAELLNALTPGDFPKKTLLANSGSEAVENAVNIA
                     RYYTKRSAVICFEGGYHGRTLLTLSLTSKYALFKKGFGPYVSDIIRLPAPNMYRKPVE
                     FTDEAYVNYCIQQFETAMIAQIDPDSVAAIIIEPVQGEGGFIPMPKAFLQKLRTVADA
                     HGIVLIFDEIQCGASRTGKLFACEHFDVVPDIICMAKSIGAGMPISAITGRATIIDAP
                     HLGGVGGTYGGNPLACVAAIEALKILSSAEFLERVNHVGKLIAITLNAWKTKYQFIGD
                     IRGLGAMQLIEFVKDRSTKEPDVEIAMEIIKEAVSNGIILIRAGLYSNCIRLLPPVVM
                     TDEQLSEGLQVLENAIRKAHEKRNA"
BASE COUNT         1486 a         1017 c          813 g         1475 t
ORIGIN      
        1 acgtttagca attacaccgg tgaaagtatt tggtaaagca aaacgatact gatcagcgcc
       61 gtcaattgca ttccaatgac aaacagcatc attagttgta attccatcaa caaataaacc
      121 acccggagtt tcgcagaata aaatttcaac acaacttaag taagcaagaa tatttgcacg
      181 taagttggca gcattttcaa gcggtgaatg gaagttattg gtagtcatac caccaaccat
      241 aacacgacca tcgccccatg gtttttctgc taatactgca gatgaaggtg aaaataaatc
      301 taccataaca ttggtatatc ctgttcctga aacaattgca tggccaaatg aaccaccggt
      361 ataatcggtg cctgcaggag caaaaggacc attaaatatt ggatgagcgg catccactgc
      421 agttgcattg tcggtaaaat aaggataagt taaagttgta ccatcgaatc caaaactcat
      481 accatcacct tcgttaggtg cagcatttaa tagaagttta ccacctgaag caacccagtt
      541 ttctatatca gcaccataag ttgcgaggaa agattctaat tcagcagcgt gactatcact
      601 accttctaaa aatacaaaac agttatccgg attaaataca gcataaacat ctgctgtttc
      661 aaaatattca cgagtccaac caattccttc tgcaccaaaa acagtgttca ttgcagttgt
      721 attagttgta gtaaaccaag gttcgccacc agttgtattt gaatacatat acactgcacc
      781 agttccggta atagggccga ggttacaaac agtttcagta taatcgtcat ttatttcagc
      841 agcatcgcca tcaactgaag tccaagcacc aatttcataa cagccatctg ctgtaaaatc
      901 atgtgttgtg gagaaagtat atgatgctgt gctatatgca gcaattgttc cagcgtataa
      961 ttcagtaact gctgcaccgc cgtcaacgtt ataactaacg ttaaatccgg acaaagattc
     1021 tgaaccgtaa ttttgaatgg taacagttac actttctaca ccaagtgtaa caccagaggt
     1081 aggagctgta acatcaacta caccaccgtc aattgcagct gcaccttcaa ttactacggt
     1141 ataatcttcc gcttctccgt aactggtgga ggcacatgga tcaagtggtg ttgctaaaaa
     1201 tgctggataa atacatctaa cgcgcatgcg tgttgcacct tccatagttc cgggagcaag
     1261 tgaaaaatca aaactgcctg ttgcacctgc aactaagcta actgagccaa gtacttcatc
     1321 cgttgaaaat acatcatcgt gatttccatc tatccaagct gtataagttt ggctaaatga
     1381 aggattatta tttaatgtca ttgtataggt tgtaccaata gtcaggtttg cagttaatcc
     1441 tgtataatcg gaatacccaa catcggtaat ggcaccacct gagccaatat ttgagagatc
     1501 actaacggtt acaccgtcaa taaaatcacc ttcggctgta ccagttgtat acgccggaat
     1561 acaatattga gaaaatgcag ttgctgacga aagcataatt ccacccgcaa taatcaatct
     1621 tctcaatgaa ggagtaaatt tgttcataag tgtttgattt aatggtttaa ttggttaatt
     1681 aaataaaaat ataataatgc tattaattca atttaataat gcagtttacc taatgtactg
     1741 ctaattctta atttcagctg aattatatta aaatccagct aaaattaaca tatatgatta
     1801 atctacaagc tttacaatta cacgctataa aatttatcat cgaccacaat tctctcaaca
     1861 tttacaagtc gctcaacgtg cattgaaaac gcaagataaa caaaaattga aaatttgtca
     1921 aaaatcatga tacaattttc aattgacagc atcaattgaa agccaaatga gtaaaaccat
     1981 ttagactaca ggcgcaccgc ctatgtatgt ttgaaccaat tctgcattgt tggttcccag
     2041 atgtttcaaa attaaaaaat cagcaaactt gccttgttca accgatccaa gctgattagc
     2101 ctgattacct gcaatagcac tgcccatggt atatgcatat aatgcctcct taaaggtaac
     2161 acgctggttt tcagctatta ctttaccttg caatgttttt cgacttattg cagattgtat
     2221 attgttaagc ggattaattt gtctaactac cggagcatct gtggaaaatg caactgtaac
     2281 acctgcttct attacattgc gcaccggata tactgtgctt aaatattgtt catctatatt
     2341 atcaataaaa ttattgccca gttcttcaat aaataccggt tgcattaccg caattactcc
     2401 gagttgatgc atcaattgta aatcactgtt atcaggaaac cccaaatgtt caattctgtg
     2461 acataacttt tgagtgtttt ctgcggcaat agacgtgtac acatctaaca ccattttaat
     2521 agcagcatct ccaatagcat gtgtagcaat tttaaaacct gcctgttgtg attctgctgc
     2581 aagtgattta aaggttgcag catccaaccg gagtacacca aatccggcat tccctttgta
     2641 tggtcgcaac aatgctgctg ttttgccact cagtcctccg tctgcaaaaa actttacggt
     2701 attcacaacc aaatgttcag actgataatg ttcaggattt ggataaacct tattagcgcc
     2761 atccggaacc cgaattggga tggcatttat tcgcaatact aattctccat tggcatccat
     2821 ttctttatac acagcaagta aatctctgtc aactgctggg tcggtggctg ccgtaatgcc
     2881 taattgcaac agatatcgtt gcgcagcaag tatcatggtt cttaattcac ttttagtata
     2941 ccttggtatt ttggataata ttaaaccaat tgccgtttcg gtaaaatgac cggaaacagt
     3001 tccgtccgga agcaatttca tttcaccgcc ggaaggaacc ggtgtatccg catttatttc
     3061 tgcaactgct aatgcttttg aattaacaat aatctgatgc gcacaagtac gggttacaca
     3121 aactggtttg tcggttggaa ttaaatccaa gtcatatttt gttggaaacc ttttatcaat
     3181 aaaattagct tcattaaatc ctcttgcctg tatccatgtt aattgaggat gttgattcgc
     3241 ataatctaat attttttcct gcatttcagc aatgctgcga accgtccgga ggtctaacat
     3301 atgtgtcatt aaattgccca ctttccaaat atgaatatgt gcatcgttaa atccaggaat
     3361 tacataattg ccctccaaat caatcaaaat atcatctgcg gattgttggg ctaaaattgt
     3421 agatgtgtcg cctacagcaa taaatttatc ttcttccaca ataaatgcat ccgcttcagg
     3481 catcagttta tcctgggtga gcacctttgc attaaagaag atttttctgc tcataaatta
     3541 cgcatttctt ttttcgtgtg ctttgcgaat agcattttct aatacttgaa ggccttcaga
     3601 taactgttca tctgtcatta ccaccggagg caataaacgg atacaattag aatataaacc
     3661 ggcgcggatt aaaataattc cattacttac agcttcttta ataatttcca ttgcaatttc
     3721 cacatccggt tcttttgttg aacggtcttt cacaaattca attaattgca tagcgcctaa
     3781 tcctcgaata tcaccaataa attgatattt tgttttccat gcattaaggg taatggcaat
     3841 taatttgcct acatgattaa cacgttccag aaattcggct gaagataaaa tttttaatgc
     3901 ttcaattgcg gcaacacaag ccagggggtt tccgccataa gtacctccta ctccacctaa
     3961 atgtggtgca tctattatag ttgctcttcc tgttatggcg ctaattggca taccagctcc
     4021 aatagatttt gccatacaaa taatatcagg aacaacatca aaatgttcgc atgcaaataa
     4081 ttttccagtg cgtgaagctc cacattgtat ttcatcaaat attaaaacaa taccatgtgc
     4141 atcagctact gttcgaagtt tttgcaaaaa tgcttttggc atgggaataa atcctccttc
     4201 accctgcaca ggttcgataa taattgctgc aacactgtcc gggtctattt gtgcaatcat
     4261 ggcagtctcg aactgttgaa tacaataatt cacataagcc tcatcagtaa attctaccgg
     4321 cttgcgatac atatttggtg caggtaatct tatgatatca ctaacgtacg gaccaaatcc
     4381 ttttttaaat aatgcatatt tacttgtcaa cgataaggtt aataatgttc gtccgtgata
     4441 tccgccttca aaacaaatta cggcactgcg ttttgtgtaa taacgggcta tgttaactgc
     4501 attttctaca gcttcactgc cactatttgc aagcaatgtt tttttcggaa aatcgcccgg
     4561 tgttaaagca tttaataatt ctgccagctc aacgtaaggt tccggtgttg ctacaagtga
     4621 acatatatga atatatttat ctaactgtgt tttcattgca tcaacaacct gtttattgct
     4681 gtgcccaata ttcatcatac caattccacc ggcaaaatcg agcaacacat tgccatcaac
     4741 gtctgttact acgccacctt ctgcatgtgc tattgccact tctgttgact t
//
LOCUS       DAORKW010000258         4786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_791_length_4786_cov_5.954132, whole genome shotgun sequence.
ACCESSION   DAORKW010000258 DAORKW010000000
VERSION     DAORKW010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_791_length_4786_cov_5.954132"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            640..2016
                     /locus_tag="PLB46_10365"
     CDS             640..2016
                     /locus_tag="PLB46_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HRF76966.1"
                     /translation="MAFKTIIEPFRIKTVEPIKMSSVAERKNYIKNAHYNPFLLKSSQ
                     VIIDMLTDSGTSAMSQNQWAAMMQGDESYAGAASWERFYDAVNDLTGFEFVLPTHQGR
                     AAERILYGYLGGKGKIFISNTHFDTTRANIEFSGATAIDIPVKEGKDFDTDYPFKGNL
                     DITLLQQLIKKHGKNIAAVIVTVTNNSGGGQPVSMENMKAVSALCKKNKLLCILDCCR
                     VAENAYFIKHREKAYANHSYRQIAQQMFALADGAIMSAKKDALVNMGGFLAVADKKLA
                     DACMNLLIITEGFSTYGGLSGRDMEAIAVGLKEVFDDDYLNYRIKSTAYLGDRIKQHG
                     VPILYPIGGHAVYVDAKKLYPQIPVHEYPGQALVCELYTTGGIRCVEIGSVMFGKYNK
                     SGKLIPAPQELVRLAIPRRVYTQSHIDYVIEVFDEVLKNSKKVKGLKITKEPQFLRHF
                     TAHFERLK"
     gene            2384..3571
                     /locus_tag="PLB46_10370"
     CDS             2384..3571
                     /locus_tag="PLB46_10370"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRF76967.1"
                     /translation="MPIYKQLGKIPDKRHIVFRDSEGKLYNEELVGTQGFSSLSSLVY
                     HIYPPTRVIKTEKPYSVAPKIAIENNMRMLSFSGFDIPQEADYIESRKVLFVNNDLHI
                     GLAAPTSTPDYFFKNADADELIFVHKGSGTLTTMFGDIDFEAHDYLVIPRGTIYKIEF
                     DTDDNRFLFIESFSPVVTPRRYRNEFGQLLEHSPFCERDIKRPVELKTYDVEGEFPIM
                     IKKRGEIFPYTYANHPFDVVGWDGYHYPYAFNIHNFEPITGRIHMPPPIHQNFEGNNF
                     VVCSFVPRLYDYHPQAIPAPYHHSNIDSDEILYYVEGNFMSRNNIKPGQLTLHPAGIP
                     HGPHPGAIERSIGQKETHELAVMIDPFKPVMITQAAVEYEVDEYYLSWIKNNGVNQNV
                     VTN"
     gene            3598..4728
                     /gene="hppD"
                     /locus_tag="PLB46_10375"
     CDS             3598..4728
                     /gene="hppD"
                     /locus_tag="PLB46_10375"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRF76968.1"
                     /translation="MDTLLVEKQTTKIQEDFLPLNGTDFIELYVGNAKQSAHFYKTAF
                     GFQSLAYSGLETGNRECASYVLRQGKITLILTTPYNSSSPVAEHIKNHGDGVKVIAFT
                     VDDAEYSFAQTTERGAEPFLFPTVKSDADGEIKIAGIKTYGEVIHLFVERKNYNGVFL
                     PGFTKWESEYNPTPIGLEYVDHCVGNVGLGRMNVWAKFYEEVLGFKNLITFDDKDIST
                     EYTALMSKVMANGNMRIKFPINEPAIGKKKSQVEEYLDFYEGEGCQHIAVATDDIIFT
                     VSELRKRGVEFLYVPGTYYDTVTERVGTINEDLQTLKGLGIMVDRDEDGYLLQIFTKP
                     VEDRPTMFFEIIERCGAKSFGKGNFQALFESIEEEQRKRGTL"
BASE COUNT         1553 a          878 c          937 g         1418 t
ORIGIN      
        1 aagcacaata tgaaatcgct taacgtggcg aaatacaaaa tcgaaattaa agctgcgggg
       61 aagatggagc agatttacaa agaacttttt gcagaggggt gaaatacaag gaacgcggat
      121 gacgcggatt tgttttgttt gaatgatgtg tgggtggatt tgcgcggatt tgtttttaga
      181 ttgattttac atttccataa tttttttgca tttaatacaa aagttagaac tttttttaaa
      241 tggaaaacag attgaactgg ttaaacggat ttaatctgtg ttaacccatt aaatccgttt
      301 aaccggtgct attttgccgt caggcaggtt ccttttcaaa cttaacattt ttaataagcg
      361 ccgcaggcat cagcccttat ccgcgttgcg aagcatccgc gtcatctgtg cctgtctgcc
      421 gccaggcagg tccctttttc aaattcaacc caatcaaaaa gcgccgcagg catcagccct
      481 tatccgcgtt acgaagtatc cgcgtcatct gcgcctgtct gccgccaggc aggttccttc
      541 tcccttccaa aattccattc aaaccaccaa actatttcct atgattttaa tcatttgata
      601 gcaatcaacc ttggttcacc tttgcctgaa atttaggtta tggcatttaa aacgattatt
      661 gagccgtttc gtattaagac ggttgagcca attaaaatga gttcggttgc ggagcggaaa
      721 aattatatta aaaacgcaca ttacaatccc ttcctcttaa aatcatcgca ggtaattatt
      781 gatatgctta ccgacagcgg cacatctgcc atgagtcaga accagtgggc tgccatgatg
      841 cagggcgatg aaagttatgc aggcgctgca agctgggaac gtttttatga tgccgtaaac
      901 gatttaacag gtttcgaatt tgttttgcct acgcaccagg gccgtgctgc tgagcgtatt
      961 ttatatggtt atttgggagg taaaggaaaa atatttatca gcaatacgca ttttgataca
     1021 accagagcaa atattgaatt tagtggcgca acagcaatcg atattcccgt aaaagaagga
     1081 aaagatttcg acaccgatta tccgtttaaa ggcaatttag atattacatt attacaacaa
     1141 ctcataaaaa aacatggaaa aaatattgct gcagtaattg ttactgtaac caataatagt
     1201 ggtggcggac aacccgtgag catggaaaac atgaaagcag tatctgcact ctgcaaaaaa
     1261 aataaattat tatgcattct cgattgttgc cgcgttgccg aaaatgctta ttttattaaa
     1321 catcgtgaaa aagcgtatgc caatcacagc taccgacaaa ttgcgcaaca aatgtttgcg
     1381 ttagccgatg gtgctattat gagtgcaaaa aaagatgctt tagtaaatat gggtggattt
     1441 ttagcggtag ccgataaaaa attagccgat gcttgtatga atttactcat catcaccgaa
     1501 ggctttagca cttacggcgg tttgagtggt cgcgatatgg aagccattgc ggtcggatta
     1561 aaagaagttt ttgacgatga ttatttaaat tatcgtatca aaagtactgc ttatttaggc
     1621 gatagaatta agcaacatgg tgtgccaatt ttatatccca ttggcggaca tgcagtatac
     1681 gttgatgcaa aaaaattata tccgcaaatt ccggtgcatg aatatcccgg acaagcattg
     1741 gtttgtgaat tgtatactac aggtggcatt cgttgcgtgg aaattggcag cgtcatgttc
     1801 ggaaaatata ataaatcagg taaattaatt cccgcacctc aggaattagt tcgcctcgct
     1861 attccgcgaa gagtttacac ccaatcgcat atcgattatg taatcgaagt tttcgacgaa
     1921 gtgctcaaaa acagcaaaaa agtaaaaggc ttaaaaatca ccaaagaacc ccaattttta
     1981 agacatttta cggcgcattt tgagcggttg aaataaggga tttaaacgga acacagatta
     2041 aacagattaa acggatttag acggattaat tttagataaa agaatttttg ggagcatcac
     2101 aatcccttcc cgctttgcgc tgcaacaaaa tgttaattaa aaataatagc caaagccggc
     2161 tgtttaatta gcacatcatc aaatcagcac attagcacat tggcatgttt tctctacaat
     2221 cgaagctttt tgcgcacttt taggaactat caaataagtt tgacacacat cccctcacca
     2281 aaaatcaccc caaaaattcc ccatattatc acctcaaaca ttgacataag tcaccccgac
     2341 cagcatacat tttcggtgaa atttgcagta aataaaaatt attatgccaa tttataagca
     2401 attaggtaaa ataccggata aacgtcatat tgttttccgc gatagcgaag gaaaattata
     2461 taatgaggaa ttagtgggca cccagggctt cagcagtttg tcgtcgctgg tgtatcatat
     2521 ttatcctcca acaagagtta ttaaaaccga aaaaccatat tcggtagcac caaaaattgc
     2581 tatcgaaaac aatatgcgca tgctgagttt cagtggtttt gacattcctc aggaggccga
     2641 ttatattgaa agcagaaaag tattgtttgt aaacaacgat ttacatattg gtttggctgc
     2701 gccaacatca acaccggatt atttttttaa aaatgcggat gccgatgaac tcatatttgt
     2761 tcataaaggt tcaggtactt taactaccat gtttggtgat atcgattttg aagcgcacga
     2821 ctatctggta attccaagag gtacgattta taaaattgaa tttgatactg atgacaatcg
     2881 ctttttgttt atcgaatcgt ttagtcctgt agttacaccg cgccgctata gaaatgaatt
     2941 cggacaatta ttggaacatt caccattttg tgaacgcgat attaaacgcc ctgtcgaatt
     3001 aaaaacctat gatgtggaag gtgaatttcc aatcatgatt aaaaaacgcg gagaaatttt
     3061 tccatacact tatgccaacc atccgttcga tgtagttggt tgggatggtt accattatcc
     3121 ttatgcattt aatattcaca attttgaacc aattacaggt cgcattcaca tgcctccgcc
     3181 aattcatcaa aattttgaag gcaacaattt tgtagtatgt tcatttgtgc caaggttata
     3241 cgattatcat ccacaggcaa ttcctgcgcc ttatcatcat agcaatatcg atagcgatga
     3301 aatattatat tatgtggaag gtaatttcat gagcagaaat aatattaaac caggtcagct
     3361 caccttacat ccagctggta ttccgcacgg accacatccg ggcgctattg aacgcagcat
     3421 tggtcaaaaa gaaacacatg aactggcagt tatgatagat ccgtttaaac ccgtaatgat
     3481 tactcaggct gcagttgaat atgaagtaga tgaatattat ttatcctgga ttaaaaacaa
     3541 tggcgtaaat caaaacgtag taacgaatta aattatatca ccaataaatt tatcacaatg
     3601 gatactttat tagtagaaaa acaaacaaca aaaattcagg aggattttct tcctttaaat
     3661 ggtaccgatt ttatcgaatt atatgttggt aatgcaaaac aatcagcaca tttttataaa
     3721 acggcatttg gatttcaatc actcgcgtat tccggattag aaacaggtaa tcgcgaatgt
     3781 gcttcttatg tattgcgtca gggtaaaatt accttgattt taactacacc atataatagc
     3841 agttcaccgg tagcagaaca tattaaaaat catggtgatg gtgtaaaagt aattgcattt
     3901 actgtagatg atgctgaata ttcatttgca caaactaccg aacgtggggc agaaccattt
     3961 ttattcccaa ccgtaaaatc agatgccgat ggcgaaatta aaattgccgg tattaaaacg
     4021 tatggggaag taattcactt atttgttgaa cgcaaaaatt acaatggtgt atttcttcca
     4081 ggctttacta aatgggaaag cgaatacaat ccaacaccaa ttggattaga atatgttgac
     4141 cattgtgttg gcaatgttgg tttaggcaga atgaatgtat gggcgaaatt ttatgaagaa
     4201 gtattaggct ttaaaaacct gattactttc gacgataaag atatttctac cgaatacact
     4261 gccttaatga gtaaagtaat ggcaaatggc aatatgcgga ttaaattccc aattaatgaa
     4321 cctgccattg gtaaaaagaa atcgcaagta gaagaatacc tcgattttta cgaaggcgaa
     4381 ggttgtcagc acattgccgt tgctaccgac gatattattt ttaccgtaag cgaattacgc
     4441 aaacgtggtg ttgaattttt atatgttccc ggcacctatt acgatactgt taccgaacgc
     4501 gtgggaacaa ttaatgaaga tttacaaacc ttaaaaggtt taggtattat ggttgaccgc
     4561 gatgaggatg gctatttatt acaaatattt actaaaccgg ttgaagaccg accaacaatg
     4621 tttttcgaaa taattgaacg ttgtggcgcc aaatcgtttg gtaaaggaaa cttccaggct
     4681 ttgtttgaaa gtattgaaga agaacaacgc aaacgcggaa ctttataaat tagtctagtt
     4741 caattaacat ttgattttga gccccgggaa accggggttt tttatt
//
LOCUS       DAORKW010000259         4775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_797_length_4775_cov_5.310593, whole genome shotgun sequence.
ACCESSION   DAORKW010000259 DAORKW010000000
VERSION     DAORKW010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_797_length_4775_cov_5.310593"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLB46_10380"
     CDS             complement(<1..420)
                     /locus_tag="PLB46_10380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF76969.1"
                     /translation="MDDSFINILVFYTFCGAAFFQLFYLLFFYLRLANYKPKEKKITQ
                     YPPVSVIICARNEDTNLKQHLPKIFAQDYPAFEVIVVDDNSDDGTTEYLFFLSQKEPR
                     LKRTKTGNSNRMMAGKKFPLTLGLKAAANELVVLTDAD"
     gene            491..1657
                     /gene="tgt"
                     /locus_tag="PLB46_10385"
     CDS             491..1657
                     /gene="tgt"
                     /locus_tag="PLB46_10385"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRF76970.1"
                     /translation="MHKLSTIKYFCGVFFQLTNTDPHSKARAGVLETDHGSIETPIFM
                     PVGTIGTVKGVHFKELEEDIAAQIILGNTYHLYLRPGTDTIEKAGGLHKFNGWKGPIL
                     TDSGGFQVHSLAQIRKIKEEGVTFQSHIDGSKHTFTPEGVMDIQRKIGADIIMAFDEC
                     TPYPCEYPYAKKSMELTHRWLQRCVTHFKATEPIYNYRQFLFPIVQGSVYPDLRRQSA
                     EVIAGMELEGNAIGGLSVGEPHEDMYAMTETVCNILPENKPRYLMGVGTPANILESIA
                     LGIDMFDCVMPTRNGRNGMLFTAEGIINIKNKKWETDFSPIDITSNASTSRLHSKAFL
                     RHLFASGERLGPQIASIHNLSFYLWLVKEARKHIIAGDFAQWKVQMVKNVSARL"
     gene            1654..2781
                     /locus_tag="PLB46_10390"
     CDS             1654..2781
                     /locus_tag="PLB46_10390"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRF76971.1"
                     /translation="MKKIWDYIFMPFRRWLSIIDIYVVKKFVVSTIFAHVIIISIAIV
                     IDLSEKTEKFVEKNAPTPEIIQYYLDFIPFITSILAPLLIFLAVIFFTSRMAYNSEII
                     AMFNSGMNFRRFLRPYIVCGLAAVGVLLYANYWTVPETNKRKLAFEDKYIHAPKNYGN
                     NKHLKINKNTFISLERFKFKNNEGYNFSLEEFKGSDKDRQLTRKINASKIQYMADTKK
                     WRFIDYKYWEIKNPGEKYTEGKQMDTVLNLLPKDFDEDIRIKDVLNYEEMEQYIAEKK
                     AEGTGELEYYTVEQYRRASSAISALILVIMGAALGSKKIRGGNWFNIIAGVALSALYI
                     FFMQFSTSFSINNGLHPIIGTNIPNILFAFVALLLVRYASR"
     gene            complement(3167..4714)
                     /gene="dnaB"
                     /locus_tag="PLB46_10395"
     CDS             complement(3167..4714)
                     /gene="dnaB"
                     /locus_tag="PLB46_10395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRF76972.1"
                     /translation="MAEYDNNQTNQYKKNDRKRNQADLSSVVYGKVPPQAPDLEEAVL
                     GAFMIDRDAVTNAIEILRAESFYDDRHRIIFAAIRRLFEKTQPIDLLTVTEELRKMGQ
                     LEEAGGPFYVTSLTNRVASAAHIEHHARIVTQKFIQRELIRISSEIVHDSYEDTTDVF
                     ELLDKAEQNLFNIADKNLRTSYNEINNLLSATIKEIDEARKHEDSLTGCPSGFSNLDR
                     VTGGWQKSDLIIVAARPAMGKTAFTLSLARNAAVDFNKGVAIFSLEMSSQQLVKRLLS
                     AETEIPAEKIIKGHLAEHEMVQLVKLSGKLGEAPIFIDDTPAINIFELRAKCRRLKMQ
                     HDIQLVVIDYLQLMTASDSNKGGGNREQEISQISRSLKSIAKELNIPIIALSQLSRKV
                     EDRGGSKRPMLSDLRESGAIEQDADLVCFLYRPEYYGLDVDENGLPTKGIGEVMIAKH
                     RNGALENVRVRFVDKFIKFVDLDEMYTPQNLTPLANVITDNKATITRKSKMDDMDNID
                     MSEEPPF"
BASE COUNT         1471 a          861 c          945 g         1498 t
ORIGIN      
        1 atctgcatcg gttaatacca ccaattcatt ggcagcagct tttaagccta atgtgagcgg
       61 gaattttttt cctgccatca tacggttgct attacctgtt ttggtacgtt ttaatctcgg
      121 ttctttttgg gaaaggaaaa agagatattc ggtagtgccg tcatcagaat tatcatctac
      181 cacaattact tcaaatgcag gatagtcctg tgcaaaaatt tttggaagat gctgtttgag
      241 attagtgtct tcgttccgtg cacaaataat tacagataca ggtggatact gcgttatttt
      301 tttttctttc ggtttataat ttgctaaacg caaataaaaa aacaggagat aaaaaagttg
      361 aaaaaaggct gctccgcaaa atgtgtaaaa tacaaggata ttaataaatg agtcgtccat
      421 aatagtgctg cggcaaagtt agattattga acttgcgcac cacatttaag ttgacaacag
      481 ttcataacat atgcacaagc tttccacaat taagtatttt tgcggcgtgt ttttccaatt
      541 aaccaatacc gatccgcatt caaaagcccg tgcaggtgtg cttgagaccg accatggcag
      601 cattgaaacg cccattttta tgcctgtcgg cactattggc accgttaaag gtgtacattt
      661 caaagagctt gaagaggata tcgctgccca gattatttta gggaatactt atcatttgta
      721 tttgaggccg ggaaccgata ccatcgaaaa agccggtggt ttacacaaat tcaacggatg
      781 gaagggaccg attttaaccg atagtggtgg ttttcaggtg cattctttag cacaaatccg
      841 gaaaatcaaa gaagaaggtg ttacgtttca gtcgcatatt gatggttcca aacatacctt
      901 tacgccggaa ggtgtgatgg acattcagcg aaaaataggt gctgatatta ttatggcatt
      961 tgatgaatgt acaccctacc cttgtgaata tccttatgcc aaaaagagta tggaactcac
     1021 acatcgttgg ttacaacgtt gtgttactca ttttaaggca actgaaccaa tttataatta
     1081 cagacaattt ttatttccaa ttgtacaggg ttcggtttat ccggatttgc gcagacagag
     1141 tgcagaagta attgcaggca tggaacttga gggtaatgcg attggcggat taagcgttgg
     1201 agagccacat gaagatatgt atgccatgac cgaaacagtg tgtaatattt taccggaaaa
     1261 caaaccccga tatttaatgg gagtaggaac accggccaat attttagaaa gtattgcgtt
     1321 aggtatcgat atgtttgatt gtgtgatgcc tacacgtaat ggcagaaatg gtatgttatt
     1381 tactgccgaa ggcataatta atattaaaaa taaaaaatgg gagaccgatt tttcgcccat
     1441 agatattaca agtaatgctt caacaagtcg tttacacagc aaagcgtttt tgcgccattt
     1501 atttgcaagt ggtgaacgac ttggaccaca aattgccagt attcataatt taagttttta
     1561 tttatggctg gtaaaagaag caagaaaaca tattatagct ggtgattttg cacaatggaa
     1621 agtgcaaatg gtaaaaaatg tttccgcgag attgtgaaaa aaatttggga ttatatattt
     1681 atgcctttca ggcgatggtt gagtattatc gacatttacg tagtaaaaaa attcgtagtg
     1741 tctaccatat tcgctcatgt cataattata tccattgcta ttgttattga cctctctgaa
     1801 aaaacagaaa agtttgtcga aaaaaatgcg ccgactcctg aaattattca atactacctt
     1861 gattttattc cgtttatcac ctccatactt gcacctttat taatattttt agcggtaata
     1921 ttctttacct cccgcatggc ttacaacagt gaaattattg ccatgtttaa cagtgggatg
     1981 aacttccgtc gctttttacg accttacatt gtatgcggtt tggctgctgt tggtgtgttg
     2041 ttgtatgcca attactggac agtgcctgaa accaataaac gaaaattagc atttgaggat
     2101 aaatatattc atgcaccgaa aaattatggc aataataaac atcttaaaat aaataaaaat
     2161 acattcattt ctttagaacg atttaaattt aaaaataacg aaggttataa tttttcactg
     2221 gaagaattta aaggtagtga taaggacagg caactgacga gaaaaattaa tgcatctaaa
     2281 atccaatata tggccgatac caaaaaatgg cggtttattg attacaaata ttgggaaatt
     2341 aaaaacccgg gtgaaaagta taccgaaggc aaacaaatgg atactgtctt aaatttatta
     2401 ccgaaagatt ttgatgagga tattcgtatt aaggatgtat tgaattatga agaaatggag
     2461 caatacattg cggagaaaaa agcggaaggt acgggtgaat tagaatatta tactgttgaa
     2521 caatatcgca gggcttcttc tgctatttct gcattaattt tggtaattat gggtgcggct
     2581 ttgggatcaa aaaaaatacg tggtggtaat tggtttaata taattgccgg tgttgcgtta
     2641 agcgcattat atattttctt catgcaattt tctacttcgt tttccattaa taatggatta
     2701 catcctatca tcgggactaa tattcccaat attttgtttg catttgtagc actgttgctc
     2761 gtgcggtatg cgagccgata gggtgaaata atatgattag cttacagaag tgcatatcaa
     2821 ttaagaaagg tttctcgcaa agtcgccaag gttaaattgc cagcacgatg tgctattttc
     2881 aaataaaaaa ccaattcaag caagcaaagg tataattggt ttaactgctt tgcgtgctta
     2941 aaaacaaacc atatcacaca tcaaatattc acctaaactt agcgcctttg cgagaaactc
     3001 ttcgataatt gtttcacgcg gagaagggag aaagcggaga gttaaataaa atggtttaca
     3061 agctttgcgt gcttaaaaac aaaccatatc gcacattatg tattcaacta tactttgcgc
     3121 ctttgcgaga aacaaccctt actctatttc agtagcctag tataaattaa aacggtggtt
     3181 cctcggacat atcgatatta tccatatcat ccatttttga tttacgggtg atagtagctt
     3241 tattatcggt gatgacatta gcgagaggag ttaaattttg aggcgtatac atttcatcaa
     3301 gatcaacgaa tttgatgaat ttatctacga aacgaacacg cacattttcc aatgcaccgt
     3361 tacgatgttt agcaatcatt acttcaccaa taccttttgt cggcaatcca ttttcatcca
     3421 catctaatcc ataatattca ggacggtaaa ggaaacacac taagtcggca tcctgctcaa
     3481 tggctcccga ctcacgtaaa tcggataaca ttggacgttt gctaccacct cgatcttcca
     3541 ctttacgtga taactgtgat aatgcgatga taggaatatt taattctttg gcgatagatt
     3601 ttaatgaacg tgatatctga ctaatttcct gttcgcgatt tccaccacct ttatttgagt
     3661 cggaggcagt cattaattgt aaataatcga ttactaccaa ttgaatatcg tgttgcattt
     3721 ttaaacggcg gcattttgcg cgtaattcaa agatgttgat ggcaggtgta tcgtcgataa
     3781 aaattggagc ctctcctaat ttacctgaaa gttttaccaa ctgcaccatt tcatgttcag
     3841 cgagatgacc tttaataatt ttttctgcag gaatttctgt ttcggcactt aataaacgtt
     3901 ttactaattg ctgagaactc atctcaagcg aaaaaatggc aacaccttta ttaaaatcaa
     3961 ctgccgcatt tctggcgagc gataaagtga aagctgtttt acccattgca ggtcgggcag
     4021 caacgataat taaatccgat ttttgccaac ctcctgtaac acggtcaagg ttactaaaac
     4081 cagaaggaca tcctgttaac gaatcttcat gttttcttgc ctcgtcaatt tcttttatcg
     4141 tagcacttaa taaattatta atttcattat aactggtgcg caagtttttg tccgcgatgt
     4201 taaataaatt ttgttcggct ttatctaaca attcaaatac gtcggtagta tcctcatagg
     4261 aatcgtgaac aatttcactt gaaatacgaa taagctcacg ctgaataaat ttttgggtaa
     4321 caatacgcgc atggtgttcg atatgcgccg ctgaagccac cctgttggta agcgatgtca
     4381 cataaaacgg acctccggct tcttccaatt gacccatttt gcgcaactct tcggttacgg
     4441 taagtaagtc gataggttgc gttttttcaa acaagcggcg aatagcggca aaaatgatgc
     4501 ggtgacggtc gtcataaaag ctttccgctc tcaaaatttc gatagcgttg gtaacggcat
     4561 cacggtcgat catgaaagcg cctaaaacag cttcttccaa atcgggtgct tgtggtggta
     4621 ctttaccata aaccacgctg gataaatctg cctggttgcg ttttcggtcg ttttttttat
     4681 attgattggt ctggttgtta tcgtattctg ccatgttgtt tcagtttgcc ggttctcccc
     4741 tgttacggga cgccaaaagt ataacgggaa acagt
//
LOCUS       DAORKW010000260         4739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_809_length_4739_cov_5.171862, whole genome shotgun sequence.
ACCESSION   DAORKW010000260 DAORKW010000000
VERSION     DAORKW010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_809_length_4739_cov_5.171862"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1788
                     /locus_tag="PLB46_10400"
     CDS             <1..1788
                     /locus_tag="PLB46_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF76973.1"
                     /translation="LNTGKVANFIITSGNIFKESTIIYENWVNGIPYTVQQQKPDVRG
                     VYKLTVDNRIYGLQISGKAAAPEFAVIKNTDTLKATGNVTESTVSIVFNDGLEIIRLS
                     GWTGDKSFGGTGQINSAWINWNATYTNVYAEKPEVAKNDSITIGNMIYPFTAYGTNSL
                     PKQQEILITNTTVWTNEEAGVLKNYDVLISNGKIIKVGKNIAAKNATVIDGTNKHLTA
                     GIIDEHSHIAVTSGVNEGTQSVSAEVRIGDVINCDDVNIYRQLSGGVTSSHILHGSAN
                     AVGGQTQLIKLRWGAAPEQMKFEGADGFIKFALGENVKQSNWGDDNTVRFPQSRMGVE
                     QVFEDAFTRAEEYIAAKNSGALVRTDLELEALVEILQEKRFITCHSYVQSEINMLMKV
                     AERHDFKVNTFTHILEGYKVADKMKMHGAGASSFSDWWAYKYEVAEAIPQNPNILHEE
                     GVVTAINSDDAEMARRLNQEAAKSIKYAGMSEEDALKMVTLNPAKLLHVDNRVGSIKE
                     GKDADLVLWSDNPLSIYAIAEKTMIDGIIYYDYSKQAAIMENAAKERARIISKMLHEK
                     NAGSPVIPISTKENRLYECDDDGDYVKGN"
     gene            1826..3103
                     /locus_tag="PLB46_10405"
     CDS             1826..3103
                     /locus_tag="PLB46_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRF76974.1"
                     /translation="MKKIFIVYLLLITTISLQAQQITIVIDNATLHIGDGNVIEKGTV
                     IFKNGKIEYAGPQKDHGFDDNTASIIDGTGKHVYPGIIATGSGLGLIEYSAVRATRDA
                     YEVGVFNPNVRSLIAYNTDSKVIPTVRSNGVLIAQVVPGGGVISGQSSIMKLSGWNWE
                     DAAVDTDNGMHMYWPGVYSYNYEKGTYEVSARYAEEVNEINTFMQEAKAYCSVENHKE
                     KNLKFEAMRELFAGKQQMFISANAAKAIVGAVDFAKAFGIKIVIVGGRQSYRVTTLLK
                     ENNIPVIIETTHELPSFDGDPVDLPYRLPGILKSAGILCGLTLSDGGDSFWGMRNIPF
                     IAGTSAAYGLTKEEALQLITLNNAKILGIDNNYGSLAAGKSATLFVAEGDVLDMRTSV
                     LTNIFINGERIKVENWQNELSKKYELKYGIEVK"
     gene            3281..3368
                     /locus_tag="PLB46_10410"
     tRNA            3281..3368
                     /locus_tag="PLB46_10410"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3315..3317,aa:Ser,seq:gga)
     gene            complement(3530..4294)
                     /locus_tag="PLB46_10415"
     CDS             complement(3530..4294)
                     /locus_tag="PLB46_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRF76975.1"
                     /translation="MIDTHAHIYLPEFANDQAGMIQRAKDAGVKKILMPGIDHEVIND
                     MMQLAESENGFCYAMMGLHPCSVKEQFEKELSIVAQHFANENHQFVAVGECGLDYYWD
                     KTFVPQQKEAFEFQIKLAKKLNLPIVIHSRESTDDCIAMIAMHKDENLRGVFHCFSGT
                     SEQLEQIIELDFYAGIGGVVTYKNGGLDKILQKRHLSHIVLETDAPYLTPVPFRGKRN
                     EPAYLTYVTNRLAAIIGVTTDEVENITTANALKLFF"
     gene            complement(4330..>4739)
                     /locus_tag="PLB46_10420"
     CDS             complement(4330..>4739)
                     /locus_tag="PLB46_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRF76976.1"
                     /translation="HAIGNHTFHHLNGWKTENAAYVNDVFACAEKVESQLFRPPYGRI
                     GFNQIQELKKHFTIVMWDVLSGDFDTTIDADTCYNNVVNNVNDGSVIVFHDSLKAMER
                     LLPALPKVIDTLLQQGYHFEPIAITNNVAIPSL"
BASE COUNT         1603 a          780 c          958 g         1398 t
ORIGIN      
        1 ttaaataccg gaaaagtggc caattttatt attacttctg gaaatatttt taaggaatca
       61 actataattt atgaaaattg ggtaaatgga attccttaca ctgttcaaca acaaaaaccg
      121 gatgttcgtg gggtatataa attaactgta gacaatcgaa tttatggctt acaaattagt
      181 ggtaaagctg ctgctcctga atttgctgta attaaaaata cagacacatt aaaagcaacg
      241 ggtaatgtaa ctgaaagcac tgtaagtatt gtatttaacg atggtttgga aatcatcaga
      301 ttaagtggtt ggaccggtga caaatcattt ggtggaacag gacaaattaa cagtgcctgg
      361 ataaattgga acgcaacata tacaaatgta tatgcagaaa aacccgaagt tgcaaaaaat
      421 gacagtatta cgataggaaa tatgatttat ccgtttactg cttatggcac aaacagtttg
      481 ccaaaacaac aggaaattct aattactaat acaacggtat ggacaaatga agaagcaggc
      541 gtattaaaaa attatgacgt attaatttcc aatggaaaaa taattaaagt aggtaaaaat
      601 attgcagcaa aaaatgccac tgtaattgat ggaacaaata aacatttaac agccggaata
      661 atagatgagc attcgcatat tgcagtaacg tctggtgtaa acgaaggaac acaatcggta
      721 agtgcagaag tgcgtattgg tgatgtaatt aattgtgatg atgtaaatat ttatcgtcaa
      781 ttaagtggcg gcgtaaccag ctcgcatatt ttacatggct cggctaatgc tgttggcgga
      841 caaacacaat taattaaatt aagatggggc gctgcaccgg agcaaatgaa atttgaaggt
      901 gctgatggat tcatcaaatt tgctttaggt gaaaacgtga aacaaagtaa ttggggcgat
      961 gataataccg tgcgtttccc gcaatcacgc atgggtgttg aacaggtttt tgaagatgct
     1021 tttacgcgcg ctgaagaata tattgcagca aaaaattcag gagcattagt gcgaaccgat
     1081 ttagagttag aagcattagt ggaaattttg caggaaaaaa gatttattac ctgccacagt
     1141 tatgtgcaaa gtgaaattaa tatgttgatg aaggttgcgg aacgtcacga ttttaaagta
     1201 aacaccttta cacatatatt agaaggatat aaagttgccg acaaaatgaa aatgcatggc
     1261 gcaggtgcat ctagtttttc agattggtgg gcatataaat atgaagtagc agaggccatt
     1321 ccgcaaaacc ccaacatttt acatgaagaa ggtgtagtaa ctgcaattaa ttccgatgat
     1381 gcagaaatgg cacgccgatt aaatcaggaa gctgccaaat cgataaaata tgcaggcatg
     1441 agcgaagaag atgcattaaa aatggtaaca ttaaatccgg caaaactatt acatgtcgac
     1501 aatcgtgtag gcagcataaa agaaggtaaa gacgccgatt tagtgttgtg gagcgataat
     1561 ccattgagta tatatgccat tgcagaaaaa acaatgattg acggaattat ttattacgat
     1621 tattctaagc aagccgcaat aatggaaaat gcagcaaaag aaagagcgag aattatttca
     1681 aagatgttgc atgaaaaaaa tgcaggcagt cctgtaattc caatttcaac aaaagaaaat
     1741 cgcttgtacg aatgcgacga cgatggcgat tatgtaaaag gcaattaact tatcattttt
     1801 gaattaaagc actcaattta ttaagatgaa aaaaatattt atcgtatatc tgttattgat
     1861 taccacaata agtttacaag cacaacaaat tactatagta attgacaatg caacgttaca
     1921 tattggtgat ggcaatgtaa ttgaaaaagg taccgtaatt tttaaaaacg gtaaaattga
     1981 atatgccggt ccgcaaaaag atcatggttt tgatgataac actgcatcca taattgacgg
     2041 cacaggcaaa catgtatacc cgggcattat tgccacaggc tcaggattag gtttaattga
     2101 atacagtgca gtacgcgcta cgcgtgatgc ttatgaggtg ggcgtattta atccaaatgt
     2161 ccgttcgcta atcgcctata ataccgactc gaaagtaatt cctaccgtta gaagtaatgg
     2221 tgttttaata gcacaagtag ttccaggtgg tggggtaatt agtggccaaa gcagtattat
     2281 gaaactctcc ggttggaatt gggaagatgc agcagttgat actgacaatg gtatgcatat
     2341 gtattggccg ggtgtctata gttataatta tgaaaaaggc acctacgaag tgagtgctag
     2401 atatgccgaa gaagtaaatg aaataaatac ttttatgcaa gaggcaaaag cttattgcag
     2461 tgttgaaaat cacaaagaaa aaaacttaaa atttgaagcc atgcgcgaat tatttgccgg
     2521 taaacaacaa atgtttattt cggcaaatgc agctaaagca atagttggag cagttgattt
     2581 cgctaaagca tttggaatta aaattgtaat tgttggtggt cgtcaaagct atcgtgttac
     2641 cacattatta aaagaaaaca atatcccggt aattattgaa accacacatg aattaccgtc
     2701 gttcgatggc gacccggttg atttgccata tcgattacca ggcattttaa aatcggcagg
     2761 tattttatgt ggtttaacat taagcgatgg aggcgatagc ttttggggaa tgcgcaatat
     2821 tccatttatt gcaggaacat ctgcggctta tggcttaaca aaagaggaag ccttacaatt
     2881 gattacactc aataatgcta aaattttggg tattgataat aattacggtt ctttagctgc
     2941 aggtaaaagc gcaacattat ttgttgccga aggcgatgtt ttagacatga gaacaagtgt
     3001 gctcaccaat atttttatca atggggaacg aattaaagtg gaaaactggc aaaatgaact
     3061 gagtaaaaaa tatgagctga aatatggtat cgaagtaaaa taacgaataa aaagcagcct
     3121 atactgttat tccatttttt ataaaagatg gtaaaaaagg tttttacaat tataatgata
     3181 tgttacgttt taggtgggct ttaagcgaat aatttaccat ctgttacagt ttgtctgata
     3241 aaacaaccat ccgttttgct taactttgcg ctaacatttt ggagagatgt ccgagtggtt
     3301 taaggagcac gcctggaaag tgtgtatacc tctaaagggt atcgagggtt cgaatccctc
     3361 tttctccgct gaataaaaaa atccgccttt tggtggattt ttttttgcta tttaattaat
     3421 attgggtatc gagagttccc tgcccgacga acataggcag acggggaatc cctctttctc
     3481 cgctgaataa aaaaatccgc cttttggtgg atttttttta tagcaaggct taaaaaaaca
     3541 gtttaagcgc atttgctgtg gtaatatttt caacttcatc tgtggtaacc ccaataattg
     3601 ctgctaaacg gtttgtcaca taagttaggt aagccggttc gttgcgtttg cctctaaaag
     3661 gcaccggtgt taaataaggt gcatccgttt ctaaaacaat atgtgacaaa tgacgctttt
     3721 gaagtatttt atctaatccc ccgtttttat aggtaaccac accaccaata ccggcataaa
     3781 aatccaattc aataatttgt tcgagttgtt cggaggtgcc ggaaaaacaa tgaaaaacac
     3841 cacgcaaatt ttcatcctta tgcatagcaa tcattgctat acaatcatct gttgactctc
     3901 gtgaatgaat aacaattggc agattgagtt ttttggccag tttaatctga aattcaaaag
     3961 cttccttttg ttgaggaaca aatgttttat cccagtaata atccaaccca cattccccta
     4021 ctgcaacaaa ctgatgattt tcgttagcaa aatgttgtgc cactatcgac aattcttttt
     4081 caaactgttc ttttacactg catggatgca gccccatcat ggcataacaa aatccatttt
     4141 cggattcggc cagttgcatc atatcgttta ttacttcatg atcaataccg ggcattaata
     4201 ttttttttac accagcatcc ttggcgcgct gaatcattcc ggcctggtcg tttgcaaatt
     4261 ccggtaaata aatatgtgca tgagtatcta tcataaaaat tagccaggtt gtattttaaa
     4321 aattaaattt caaagtgaag gaatagctac attgttggtt atagctattg gttcaaaatg
     4381 gtatccttgt tgtaataacg tatcaattac tttaggtaag gcaggtaaaa ggcgttccat
     4441 ggctttcagg ctatcgtgaa atacaattac ggagccatcg tttacattat ttacaacatt
     4501 gttataacaa gtatcagcat caatcgtggt atcaaaatcg ccgcttaata cgtcccacat
     4561 cacaatagta aaatgtttct ttaattcctg aatttgatta aatcctattc gcccgtatgg
     4621 tggtcgaaac aattgacttt caactttttc tgcgcaagca aaaacatcat ttacataagc
     4681 cgcattttcg gttttccagc cattcaggtg atgaaaggta tggttgccaa ttgcatgac
//
LOCUS       DAORKW010000261         4737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_810_length_4737_cov_5.601452, whole genome shotgun sequence.
ACCESSION   DAORKW010000261 DAORKW010000000
VERSION     DAORKW010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_810_length_4737_cov_5.601452"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLB46_10425"
     CDS             <1..237
                     /locus_tag="PLB46_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76977.1"
                     /translation="KDTCNFKASRTIYFNKPNPNYEWAFFVPFEAKKTYDILPLKFTK
                     GNWYEIKNICDPYYYIYIHVMDNNKWKIKKVERW"
     gene            665..1075
                     /locus_tag="PLB46_10430"
     CDS             665..1075
                     /locus_tag="PLB46_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76978.1"
                     /translation="MIGHTGCEYPLILRSSDSLDSSDYKISKTLMLTDDSLSQTGVIY
                     KRRSFTSSDDNYFLYLYSKGKVEVYNNCSGKDFEFCKEVEELYSFTDSSKLFFEVYYF
                     GNKHFYEWKGILEDEDLVIYRIHYRNQIGWKKLK"
     gene            1363..2496
                     /locus_tag="PLB46_10435"
     CDS             1363..2496
                     /locus_tag="PLB46_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76979.1"
                     /translation="MLTTRLDKRLSNIDHDPFGMLLEGRNWEGGSEYRFGFNTQEQDD
                     EVYGNGNLNTALFWEYDTRLGRRWNVDPVIKSFEAGYVSFNNSPINKIDIKGNNADWF
                     KGKAADGTSVPLTEFSDHTEQTFTLDGVTYTNMGTTYSEAILNLANSQAINLNNMELQ
                     LAPCCEGYMDSEMYGVYLRGKGVVEKAEEITSSTVGLLDLSSTLLELTAEAIVAYRSG
                     YGYSVATNGRTYLRPLLPNGEYRHIGNKYFTTRTSFMPPDTKLTYMLKYGSKTLEVAS
                     YVGTATELGIIWYQYSQNNISAERATYNTTFSVGSIVTSAFIGAEIGFVPGFVAGVTV
                     STAGQVGEWSYDSYIYPEIVIPSIANFNSTNWSDWWKTLGPNF"
     gene            2518..3021
                     /locus_tag="PLB46_10440"
     CDS             2518..3021
                     /locus_tag="PLB46_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76980.1"
                     /translation="MNFKTSTYSIILHLFPVILGIILAIIILVVRHLNVHFTSELILI
                     LAIIITGLILPYLYILFNHLYFSSGKSFTIEDEFKRFSYHYQNKVYKFGISDISGIQI
                     VGSFTRGFFVPGLIFKYVKININNGNQFIVTSLMMPQMEDLFTGETVKVNSFFPYLSV
                     KSLIIKT"
     gene            3242..4411
                     /locus_tag="PLB46_10445"
     CDS             3242..4411
                     /locus_tag="PLB46_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRF76981.1"
                     /translation="MNQNHHDPFGMLLVGRNYSITNENYRFGFNGYENTDELLSDNIA
                     IDFGARMYDSRLGRFLSIDPRSGEYPWQTPYAYFSNSPIAIVDIDGMGGPNKDGYTDP
                     NGNNFDLDSRYLTQTDDKSLIINTCPSGNWTYAEWVQVDEGFGYYDVGGISEDNITIN
                     YSSKWNSSYQQGSLWVSDLGYEAIFPNDHNPPAGWSYSGSGIYSTSGRTNMSHILANS
                     STPSLWMPNTRQSLMVKDAFRDGAKDAAIFWVAGITLPFVIAYTPSAFVSVQNGFYNT
                     LAWSIFRWQAISGTISGGSATYVMIGERMERVRLAALNYQAATINMPSVLTSNVKGME
                     TYNSLWFQYMLRNGSQVLSGGRASYGNVSSFYLLELQLYYRYATEINTFLQLPYK"
     gene            4418..>4737
                     /locus_tag="PLB46_10450"
     CDS             4418..>4737
                     /locus_tag="PLB46_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76982.1"
                     /translation="MNDRLVIKLIKKHYSFLIKKGFVENASPDINFCKLIAFVKDKKA
                     IVFCNDVRDRLLEVGIYTIIDGKISTHSEDITRLYELTENAKFDEIYNYDLFPLEHVI
                     SNGA"
BASE COUNT         1634 a          713 c          835 g         1555 t
ORIGIN      
        1 aaggatactt gtaacttcaa agcctccaga acaatttatt ttaataaacc taatcctaat
       61 tatgaatggg cattttttgt gccttttgag gctaaaaaaa cctacgacat attaccactt
      121 aagtttacaa aaggaaactg gtacgaaata aaaaatattt gtgacccgta ttattacata
      181 tatattcatg taatggataa taataagtgg aaaattaaaa aggttgaaag gtggtaaata
      241 accactccaa ccaaacatca atgctgtttc tgttagagaa tcaacgattt cattcaaaag
      301 ttgccattgg aaaccggcga caactggata ttcattcttc gtattgaaat ttaaaacttt
      361 accttattgt aattattaca atatctccgt tcagccgaaa ctatgattcg gcagttgcgt
      421 gccaggaccc agtccgacgc aaaatacaaa aactaaaaat cagctttcat aattaaactt
      481 gtcagttaaa cgaatgatgc cacccgcggt taactctgtt attagcattg ctaacaaaga
      541 aatatttttt aataaaagca ggccaccaaa ttaattaaat gctaatttta acacatccaa
      601 aaaattggaa gcttactaaa atatttctta caaaatgtta agaatagtaa taatatttat
      661 ttttttgata gggcacaccg gatgtgaata tccactaata ttgcgaagca gtgatagttt
      721 ggattctagt gattacaaaa taagtaaaac gctaatgctc acagatgatt cattatcaca
      781 aaccggagtt atttataaaa gaagatcttt tacttcaagt gacgataact attttttata
      841 cctatattcg aaaggtaaag tagaagttta taataattgt tcaggcaagg attttgaatt
      901 ttgtaaagaa gttgaagaat tatattcgtt tactgattcc tccaaactct tttttgaagt
      961 ctattatttc ggaaataaac acttctacga atggaagggt atattagaag acgaagatct
     1021 tgtgatttat agaattcatt accgaaacca aattgggtgg aaaaaactaa aataatttat
     1081 cagaagtatt caacctttta aatattaata agattcttta taataaggta acgaagcttt
     1141 ttggacttca taacttttta atagctgtgt cccttctccc ttagatcatc cctaaatttt
     1201 gagcagtaac caggtaataa aaaaaaacca ttcatatatc aagtaggtag gcccaaaaaa
     1261 gttccaacca cctaaaatta gataagtgga aactagattc taaaaccaat aaaaataatc
     1321 acacatactt caactttaac taatgttaag ttacttttaa gtatgcttac taccagatta
     1381 gacaagcgtt tgagcaatat tgaccacgac ccgttcggga tgttgttgga aggacgaaat
     1441 tgggaagggg gaagtgagta taggttcggg tttaatacgc aggaacaaga tgatgaagta
     1501 tatggaaatg ggaatttgaa tactgcactt ttttgggaat atgatactag attggggaga
     1561 aggtggaatg tagatccagt aattaaatcc tttgaagcgg gttatgtttc atttaataat
     1621 agcccgatta ataagataga tataaagggt aataacgctg attggtttaa aggaaaagca
     1681 gctgatggta catctgttcc tttaactgaa ttttctgacc atacagagca aacatttaca
     1741 ttggatggag taacttacac aaacatggga accacttata gcgaagcaat tttaaacttg
     1801 gctaattctc aggcaattaa tttaaataat atggaattac agctggcacc atgttgtgaa
     1861 ggatatatgg acagtgaaat gtatggcgtg tatcttcgtg gtaaaggagt ggttgaaaaa
     1921 gccgaagaaa ttacttcatc aacagtaggt ttattagacc tttcaagcac cttgttagag
     1981 cttactgcag aagcaattgt agcataccga tctgggtatg gttattcagt tgcaacaaat
     2041 ggtagaacat atttacgtcc attactacca aacggtgaat ataggcatat aggaaacaaa
     2101 tatttcacta ctagaacttc ttttatgcca ccagatacta aattaacata tatgttaaaa
     2161 tatggctcaa aaaccctaga agttgcatct tacgtgggca cagctacaga attaggaata
     2221 atttggtacc aatattctca aaataatatc tctgcagaac gagctactta taacacaacc
     2281 tttagtgttg gatctattgt tacttcggca tttatagggg ctgaaatagg gtttgttccc
     2341 ggatttgtag ctggcgtgac tgtatcaact gcaggtcagg ttggcgaatg gtcatatgac
     2401 tcatatattt acccagaaat agtcataccc tctatagcca attttaattc tactaattgg
     2461 agtgattggt ggaaaacttt aggacccaat ttttaacctt atcaacttag taattatatg
     2521 aattttaaaa cttcaacata ctcaattatt ttgcatttat tcccagtaat tctaggaata
     2581 attttagcaa ttattattct tgttgttagg cacttgaatg ttcatttcac ttccgaactc
     2641 attttgatat tagcaattat aattactggt ttaattttac cctatttgta tattttattt
     2701 aatcaccttt attttagcag tggaaaaagt tttacaattg aagatgagtt caaacgtttt
     2761 tcatatcatt atcaaaataa agtttataaa tttggtattt ctgacatttc aggtatacaa
     2821 attgttggta gctttactcg tggttttttt gtaccaggtc taatttttaa gtatgttaaa
     2881 atcaatatca ataatgggaa tcaatttata gtaacatcat taatgatgcc tcaaatggaa
     2941 gatttattta ctggagaaac tgtcaaggtt aactcattct ttccatattt atcagtgaaa
     3001 tctctcatta ttaaaacata atcagcatac aaaagcattt ttcaattaca aatcaaagta
     3061 ttttctttat ataaataatt tactatgtaa tttgtaagtg cagtaaaaaa tttaaaaatg
     3121 cctaaactta gattcgttta aattaaagtc taaaatcaac tatgaaaatc acccaaagtt
     3181 tctggtattt gcaaattttc agttatattt aagcgtgata cccaccaaaa aggacaggtg
     3241 tttgaaccaa aaccaccacg acccgttcgg gatgttgttg gtgggtagaa attattcaat
     3301 taccaatgaa aattatcgct tcggattcaa tggatatgaa aacacagatg aattacttag
     3361 tgataatatc gcaatagatt ttggtgcaag gatgtatgat tctcgattgg gtaggttttt
     3421 atccattgat ccacggtcag gagaataccc ttggcagacc ccttacgcct atttttcaaa
     3481 ttcaccaatt gctattgttg atattgatgg tatgggtggt cccaacaaag atggttatac
     3541 agacccaaat ggtaataatt ttgaccttga cagccgatat cttactcaaa ccgatgataa
     3601 atccttaatt attaatacat gcccatcagg taattggact tatgcagagt gggttcaagt
     3661 tgatgaaggg tttggttatt atgatgttgg tggtataagt gaggataata ttacaatcaa
     3721 ttattcatca aaatggaact ctagttacca acaaggctca ctttgggtat cagacttggg
     3781 atatgaagct atttttccta atgaccataa tcctcctgcg ggatggtcat attctggatc
     3841 aggaatttat tcaacatccg gtagaacaaa tatgtcacat atacttgcga atagttctac
     3901 gccgagccta tggatgccaa atacccgtca atctttgatg gtaaaagatg cattccgaga
     3961 cggtgcgaag gatgctgcta tattttgggt ggctggaatt actttgccat ttgttattgc
     4021 ctatacacct agcgcttttg tttctgtaca aaatggcttt tataatactt tagcatggtc
     4081 catattcaga tggcaagcaa tctctggaac tatttcaggg gggtctgcaa cgtatgtaat
     4141 gattggagaa cggatggaaa gagtaagatt agcagcatta aattatcaag cggcaacaat
     4201 aaacatgcct tcagttttaa ctagcaatgt aaagggtatg gagacttaca atagtttgtg
     4261 gtttcaatat atgcttagaa atggaagtca agttttaagt ggtggacgtg ctagttatgg
     4321 taatgtgagt tcattttatt tattagagct gcaactttac tatagatatg caactgaaat
     4381 aaatacgttt ttacaattac catataaata ataaaaaatg aatgatagat tggtcataaa
     4441 gcttattaaa aaacattatt cttttttaat aaaaaaaggg tttgttgaaa atgcttcgcc
     4501 agatattaat ttttgcaaat taattgcttt tgtaaaagat aagaaagcaa tagttttttg
     4561 taatgatgtt agagatagat tattggaagt gggcatttat acaattattg atggtaaaat
     4621 tagcacccat agtgaagaca taacaagatt gtatgaatta actgaaaatg ccaaatttga
     4681 tgaaatatat aattatgatt tgtttccatt agagcatgta atttcaaatg gcgccat
//
LOCUS       DAORKW010000262         4733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_811_length_4733_cov_5.113510, whole genome shotgun sequence.
ACCESSION   DAORKW010000262 DAORKW010000000
VERSION     DAORKW010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_811_length_4733_cov_5.113510"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLB46_10455"
     CDS             <1..271
                     /locus_tag="PLB46_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76983.1"
                     /translation="GGRWGDEIISSCHHGGNPADTHTNCVNPGCHLLFIQCPTCAEKF
                     AGCCSQECYDIIQLPEEEQKKLRQGIDKGQNIFNKSRVRMNAIRH"
     gene            448..1701
                     /gene="metK"
                     /locus_tag="PLB46_10460"
     CDS             448..1701
                     /gene="metK"
                     /locus_tag="PLB46_10460"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRF76984.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDNFLAFDPSSKVACETL
                     VTTGQVVLAGEVKSKTYLDVQNIARDVIAKIGYTKSEYMFEAKSCGILSAIHEQSPDI
                     NMGVERKKKEDQGAGDQGMMFGYACRETDNYMPLSLEIAHLLLIELAKIRREGKVMKY
                     LRPDSKSQVTIEYADDKSPLRIDTIVISTQHDDFGEEKKMLAEIKNDVAEILIPRVKK
                     LLPKRIQKLFDGTHVLHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYAVRHIAKNMVAAGLCDEVLVQVAYAIGVAKPVGLYIDTYGTAKVNM
                     QDGEIAKKINKIFDLRPYAIEQRFKLRTPIYAETAAYGHMGREPKIVTKTFTDVSGRS
                     IKVKVELFPWEKTDYVADIKKAFKL"
     gene            complement(2310..3176)
                     /locus_tag="PLB46_10465"
     CDS             complement(2310..3176)
                     /locus_tag="PLB46_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF76985.1"
                     /translation="MRQLKITASITSRENDSLEKYLSEIAKIDMITPEEEAELARKIR
                     TGDELALDKLTKANLRFVVSVAKQYQGHGLTLSDLINEGNVGLIKAAKKFDETKGFKF
                     ISYAVWWIRQSIMLAIVEHSRLIRLPLNKAGNVSKVNKAVSAFEQEFEREPTSAELAE
                     VLHLKDADVKDIIISNIKHVSMDAPLGGDNEDGSMAEILEDKGEDTPDHSLINESLKN
                     EIATALKVLSEREAEVVAAYFGINGFAAMSVDEIAQKFNLSRERVRQIKERAIRRLRK
                     ATTSNVLKLYLG"
     gene            complement(3371..>4733)
                     /locus_tag="PLB46_10470"
     CDS             complement(3371..>4733)
                     /locus_tag="PLB46_10470"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRF76986.1"
                     /translation="PSKKEERGEAFKLVKEEFKAQFTEAELEEKGALISTYYHDIEKH
                     AMREMVLSDRQRLDGRKLDEVRPIWGEVDYLPSAHGSAIFTRGETQSLTTVTLGSKMD
                     EQLIDGVVHEGNSKFLLHYNFPPFSTGEAKPMRGLGRREVGHGNLAMRAIKWSLPEDN
                     AYTIRVVSDILESNGSSSMATVCAGSLALMDAGVKVKKPVAGIAMGLISEPNMVRYAI
                     LSDILGDEDHLGDMDFKVAGTEAGITATQMDIKVDGLSFGVVKEALEQARRGRLHILG
                     EMAKVMTQPREDYKPHAPRIVQMQIPREFIGAVIGPGGKIIQEMQRETGAEISIEEVG
                     EFGMIDISSPNKESIDKAVNRIKGIVAVPEVGDVYEATVKSIMPYGAFVEFLPGKEGL
                     LHISEVSWQRLETLEGVINKGDKLKVKLLEIDKKTGKFKLSRKVLLDRPQKQASANEE
                     NQG"
BASE COUNT         1434 a          949 c          846 g         1504 t
ORIGIN      
        1 gggggggcgt tggggtgatg aaattatttc cagttgtcac catggtggca accctgccga
       61 tacacatacc aattgtgtta atcctggatg tcatttgtta ttcattcagt gccctacctg
      121 cgccgaaaaa tttgcaggct gttgttcaca ggaatgttat gatattattc agcttccgga
      181 ggaagaacaa aaaaaactac gtcagggcat cgataaaggt caaaacattt ttaataagtc
      241 gcgggtgcgg atgaatgcta tccgtcatta aacgacaatt gctattgtaa tcttcctttg
      301 atttacagca acgggcactg taatccctgc ttctcgccga gggttggtgg attgggtgtg
      361 taatactaaa agcaaatgtt cagataattg tgtcattacg aaccaagaaa gtatctttgc
      421 cgttcatcat aaaaataacg cttaaacatg ccatatttat tcacttctga gagtgtgagc
      481 gagggacatc ccgacaaggt tgcggaccag atctctgatg ctttaatcga caacttttta
      541 gctttcgacc caagcagcaa ggtagcctgt gaaacgctgg taactaccgg tcaggttgtg
      601 cttgcaggag aggtaaaaag taaaacctac ctcgacgtgc aaaatattgc ccgtgatgta
      661 attgctaaaa tcggttatac caagagtgaa tatatgtttg aagcaaaatc gtgcggtatt
      721 ttgtctgcca ttcacgaaca aagccctgat atcaatatgg gcgttgaacg caaaaagaaa
      781 gaagaccagg gcgccggcga ccagggtatg atgttcggtt atgcttgtcg cgaaaccgat
      841 aattacatgc ctttatcact cgaaattgca catttgttgc tcatagaact tgcgaaaatt
      901 cgtcgcgaag gcaaagtgat gaaatattta cgtcccgatt ctaaaagtca ggtgactatt
      961 gaatacgctg atgataaatc accattgcgt attgatacca tcgttatctc aacgcaacat
     1021 gatgattttg gtgaagagaa aaaaatgttg gcagagatta aaaatgatgt agctgaaata
     1081 ttaattccac gtgttaaaaa gttattgcca aaacgtattc agaaattatt tgatggcaca
     1141 catgttttac acgttaaccc gacaggcaaa tttgttattg gtggtccaca cggagatacc
     1201 ggattaacag gaagaaaaat tattgtagat acatacggtg gtaaaggtgc tcacggtggt
     1261 ggtgcattta gtggtaaaga cccatctaaa gtagaccgct ctgcagccta tgctgtgcgt
     1321 catattgcaa aaaatatggt ggctgcaggt ttatgtgatg aagtattggt gcaggttgct
     1381 tatgccattg gtgttgccaa accggtgggt ttatacatcg acacatatgg tacagcaaaa
     1441 gtgaatatgc aagatggtga gattgcgaaa aaaattaata aaatattcga cttacgtcca
     1501 tatgcaattg aacaacgttt caaattgcgc acaccaattt atgcagaaac tgcagcttat
     1561 ggtcatatgg gacgtgaacc taaaattgta accaaaacat ttaccgatgt aagtggtcgc
     1621 agcattaaag taaaagttga acttttccct tgggaaaaaa cagattatgt tgctgatata
     1681 aaaaaggcat ttaaattata atgctgttgc aaataaaaaa caaaccctga ttgtaaacaa
     1741 tcagggtttg ttttttttat atggtgtgaa ttttccaaaa taatgtcacc cctgcctagt
     1801 cagtcaggta aacctaacgg ggttggattt ttctttattg tgaatttttt ttgctaaaat
     1861 aatttcaccc ctaaaggggt tggttttttc ttcgctgtga atttttccaa aataatgtca
     1921 cccctgccta gtcagtcagg taaacctaat ggggttggtt ttttcttaga aatataaatt
     1981 tgatgtcatc attataattt gaaataatta ttaatcgtaa ttaaaccttg ttgggtttaa
     2041 ctaattgact aggcaggggt gaaattattt taacttcttg caacggtgag actcacacaa
     2101 accaaccccg ttaggggtga tattatttta actctaagga tactctaggc ccacataacc
     2161 ccgttagggg tggcattatt ttaacaataa cactaaggaa gaacaaaccc cgttaggggt
     2221 gacattatat ttcgggtatt attttgctta tcgctaacgc aatggtttgc gcgagctttg
     2281 ggatgtaaca cggttttgag gttcaacaat tatcccaaat acaatttcaa cacattactc
     2341 gtagttgctt tgcgcaaacg tctgatggcg cgttctttaa tttggcgcac acgttcacga
     2401 gataaattaa atttttgtgc aatctcatct acactcatag cagcaaaacc attaatacca
     2461 aaatacgctg ctactacttc tgcttctcgt tccgataata cttttaatgc cgttgcaatt
     2521 tcatttttca gcgattcatt gattaatgaa tgatcaggtg tatcttcacc tttgtcttcc
     2581 agaatttctg ccattgaacc atcttcatta tctcctccca aaggggcatc catggaaaca
     2641 tgtttaatat tacttataat gatatcttta acatcagcat cttttaaatg taatacttca
     2701 gctaattcgg cagatgttgg ttcgcgttca aattcctgtt cgaaagcact aactgcttta
     2761 tttacttttg aaacattacc tgctttattt aatggcaaac gaattaatct gctgtgttca
     2821 acaatggcta acataataga ttgacgaatc caccaaacag cataggaaat aaatttaaat
     2881 ccttttgttt catcaaactt ttttgccgct ttaattaatc caacattacc ttcattaatt
     2941 aaatcactta aagtaagtcc gtgaccctga tattgtttag caacagaaac aacaaaacgc
     3001 aaatttgctt ttgttagttt atctaatgcc aactcatcac cggtgcgaat tttacgcgct
     3061 aattcagctt cctcttcagg cgtaatcata tcaatttttg caatctcaga gagatacttt
     3121 tccaaagaat cgttttctct gctggtaatg ctggcggtta tttttagttg gcgcatgtca
     3181 gtgatttaac gatttatacg tttctaaatt acaaaagtta tgacatttta tcaactttta
     3241 cactttttac ccaaaaatga taaaacatta atccgctcaa aatttgtgca acttcctatt
     3301 aacgtgcact taacacaaaa aaaaatggga tgctgttgtc aacatcccat cgtattcgtt
     3361 taaaaaaaaa ttatccttga ttttcttcat tagccgacgc ttgtttttgc ggacggtcta
     3421 ataacacttt gcgtgataat ttaaatttac cggtcttttt atcgatttcc aataatttca
     3481 cttttaactt atcgccttta ttgataacac cttccagtgt ttctaaacgc tgccagctaa
     3541 cttcagaaat atgtaataaa ccttctttac caggtaaaaa ctcaacaaat gcgccataag
     3601 gcatgattga tttcacagta gcctcgtaca catcaccaac ttccggaact gccacaatgc
     3661 ctttaatgcg atttactgct ttatcgatgg attctttatt tggagatgaa atgtctatca
     3721 taccaaattc accaacttct tcaatcgaaa tttcagcacc tgtttcacgc tgcatttcct
     3781 gaataatttt tccaccgggc ccgataactg caccaataaa ttctcttgga atttgcattt
     3841 gtacaatacg cggagcatgt ggtttgtaat cttcacgagg ttgggtcatc actttagcca
     3901 tttcgcctaa aatatgtaaa cgacctctgc gcgcttgttc taaagcttct tttaccacac
     3961 caaaactcaa accatctact ttaatatcca tttgagttgc cgtaatacct gcttcagttc
     4021 ctgcaacttt aaagtccata tcacctaaat gatcttcatc accaagaata tcgcttaaaa
     4081 tggcataacg caccatatta ggttcagaaa ttaaacccat ggcaatacct gccaccggtt
     4141 ttttcacttt aacaccggca tccattaatg ctaatgaacc tgcacaaaca gttgccattg
     4201 atgatgaacc attcgattct aaaatatcag atactacacg tatagtgtaa gcattatctt
     4261 ccggtaacga ccatttgata gcgcgcatgg ctaaattacc atgaccaact tctctcctac
     4321 ctaaaccacg cattggtttt gcttcacctg tcgaaaatgg cgggaaatta tagtgtaata
     4381 aaaatttaga attgccttca tgtacaacac cgtcgattaa ctgctcatcc attttactac
     4441 ctaatgttac cgtggttaat gattgtgttt caccacgtgt aaaaattgca gaaccgtgtg
     4501 cagatggtaa ataatcaact tcaccccaga taggacgaac ttcatctaat ttacgaccat
     4561 ctaaacgctg acgatcactc aacaccattt cacgcatagc atgtttttcg atgtcgtgat
     4621 aatacgttga aattaatgca cctttttctt ctaattcagc ttccgtaaat tgtgctttaa
     4681 attcttcttt taccaattta aatgcttctc cacgttcttc ttttttagaa ggc
//
LOCUS       DAORKW010000263         4733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_812_length_4733_cov_4.874305, whole genome shotgun sequence.
ACCESSION   DAORKW010000263 DAORKW010000000
VERSION     DAORKW010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_812_length_4733_cov_4.874305"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1955
                     /locus_tag="PLB46_10475"
     CDS             96..1955
                     /locus_tag="PLB46_10475"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRF76987.1"
                     /translation="MKKLFSVLSFTVVFAAGTSYVSGQTNIFPASGSAGIGTVSPNAK
                     SLLDVQSTTAGVLIPRMTAAQRTAIISPPQGLMVYQTTIPEGIYFYNTSGWVALSPSG
                     FNNDLLPAIAGTFNLGSNTNTWENLHLGNGIYHNGSQIIGISSSSLAFGKNSMDALDA
                     ANNIALGNDALSNITTGNTNIAIGNESLTSTTTGSRNTGIGFESLNLLTTGIDNTAIG
                     NKSLNVATTASFNTSIGANTFLFLTTAAYNLAVGCNAGYNNLSGNNNAYSGAYSGYEN
                     TLGSHNSGHGYSSLRENVTGSYNTAIGYYAGSVSNSFTTGTFVGSNAYTTATGVSNLT
                     GIGNNARPTASNQVRIGNSSVTSIGGYAGWTNLSDGRFKTDVAENVVGLDFILQLRPV
                     TYHLNIDKLSNYLGETNQDDQVETAARKSKQAMVYTGFIAQEVAAAANEVGFDFSGVD
                     YPKNETDMYGLRYAEFVVPIVKSIQEMYTTSTQQESMIVDLNDKILELEKQNEQLMEN
                     ISAINELLGIHKTNEKLAITIQQNDIAKLEQNVPNPFSNSTTITCFIPNDVKTAVILI
                     QSANGVLIKEIVLSAKGHNQVQLDAESLPSGTFSYSLVLDGKVVSTKTMIYVK"
     gene            2110..3204
                     /locus_tag="PLB46_10480"
     CDS             2110..3204
                     /locus_tag="PLB46_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76988.1"
                     /translation="MKNIRTTQPEIKQVALQQRLKNMLDLTIAKVAAHVKAPQQYAMP
                     ADSSALEHKILRFYNVLKPHEKETFLAKTNLLFNQSATENRKRFEDLMDIDIKSSTPM
                     LDKIKAFPLPVKYHFTEKEATAIIEKSAKLKTIQKTKSGLTIAPQQAAEPRTLSLFLE
                     SVKCNDPQDVLKDEIFFSGFTLDALGVNEPIPFTSAGKYKKNDFKQLQIKLRDFDLRA
                     AGVFPQDFLLALFVFEKDSKKDDERLKFTMELIQDIGLWVLAAADVIAIAALVVTIAS
                     TSPVGMALIIASGVIAAIGLSIIGVISLINNLRLAEISLQVTDLFTFPDPPINVGEQV
                     INKHQVVLAGSLSGVMKGNYDLQFRWERTA"
     gene            3263..3751
                     /locus_tag="PLB46_10485"
     CDS             3263..3751
                     /locus_tag="PLB46_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76989.1"
                     /translation="MKKSYFILLLCGLSLMFAGCPYGADVPIDKEPTIPIDKTLLGKW
                     ENSSASDYTYYFRADDANTYRISKISSSTGDSTVYFAFESKIDNVSFINIYEENEFGS
                     KTYYLYKLTKRGTSKISMEEVTDNIDEKFDTSEELKAYLAKHQHLSFLFNKDPDEYVR
                     MD"
     gene            complement(3873..>4733)
                     /locus_tag="PLB46_10490"
     CDS             complement(3873..>4733)
                     /locus_tag="PLB46_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76990.1"
                     /translation="VLNDDGSLVVAGYSYSPATDYKNENPIGLSDIWVYKMYADICPL
                     HVAVTPTSDTTFCKPGSVTLTTQYDATLTYQWRRNGSNIAGATTNIYTANKTGNYTVK
                     ISNGICTKVAQEVQVTANPKPVVSINNVDGTNNLCVDASIKLKTSNGPGYTFQWYLDD
                     VPIVGANTNIFNATIIGNYKVNVTNASGCSNTSAGYNIINACKLEQLTQVAIYPNPFT
                     EVIAIVIPENEVKIDNFILTDITGKIVYTNKISKSNETFYLTFLPTGLYFIQFYAADK
                     FLFGQTIIKN"
BASE COUNT         1605 a          822 c          870 g         1436 t
ORIGIN      
        1 aaatataagg tattttctac accttactta tgcaaaatat tgattattac acatttaact
       61 tacgctgcac ctttggtaga ctaaaaccaa acattatgaa aaaactattt tctgttttat
      121 cgttcactgt cgttttcgca gccggtacaa gttatgttag cgggcaaacg aatatcttcc
      181 cggcaagtgg aagcgccgga ataggcactg ttagcccgaa tgcgaagtca ttactcgacg
      241 tacaaagcac tactgccggg gttttgatac caagaatgac agcagcacaa cgcacagcaa
      301 ttatttcacc accacaaggt ttaatggttt accaaactac aatacccgaa ggcatttatt
      361 tttataatac ttccggatgg gttgcacttt caccttccgg ttttaataat gatttattgc
      421 cggcaattgc aggtaccttc aatttaggtt caaatactaa tacctgggaa aatttacatt
      481 taggtaatgg tatatatcat aacggttcgc aaattattgg cataagtagt tccagtttag
      541 cttttggaaa gaattcgatg gatgcgctgg acgcggctaa taatattgca ctagggaatg
      601 atgcgttgtc gaatattacc actggaaaca ccaatattgc gataggaaat gaatcgctaa
      661 cctccactac aaccggttca cgcaacacag gcatcggttt cgaaagttta aacttattaa
      721 ccacgggaat tgacaataca gccatcggca acaaaagttt gaatgttgct actacagcca
      781 gttttaacac ttcaattggt gcaaacacat ttttattttt aaccacagcg gcttataatc
      841 ttgcggttgg atgtaacgcc ggatacaata atttgtccgg aaataacaat gcctattcag
      901 gcgcatattc aggttatgaa aacacactgg gaagccataa tagtggccac ggttacagta
      961 gtttgcgaga aaacgtaacg ggttcataca atactgcaat tggttattat gcaggaagtg
     1021 tcagtaattc tttcaccacc ggaacatttg ttggctccaa cgcttatact acggctacgg
     1081 gtgtaagtaa tttaacagga ataggtaata atgcacgtcc aaccgcgtcg aaccaagtgc
     1141 gtattggtaa tagcagtgtt acttcgattg gaggttatgc agggtggact aatttaagcg
     1201 atggcagatt taaaactgat gtagcagaaa atgtagttgg attagatttt attttacaat
     1261 tacgtccggt tacctaccat ttaaacattg ataaattatc taattattta ggtgaaacta
     1321 atcaggatga tcaggtagaa acggctgcca gaaaatcaaa acaagcaatg gtttacacag
     1381 gtttcattgc tcaggaagta gctgcggcag caaatgaagt tggttttgat tttagcggcg
     1441 tagactaccc aaaaaacgaa acggacatgt atggattaag atatgccgaa tttgtggtac
     1501 caatagttaa atccattcaa gaaatgtata ctacttcaac acaacaagaa agtatgattg
     1561 ttgatttaaa tgataaaatt ctagaattgg aaaaacaaaa tgaacaatta atggaaaata
     1621 tttcagctat taatgaatta ttgggaatcc ataaaacaaa tgaaaaatta gcaattacca
     1681 ttcaacaaaa cgacattgca aaactggaac aaaatgtgcc aaacccattc agcaattcaa
     1741 ccacaataac ctgttttatt cctaacgatg ttaaaacagc agttatatta attcaatcgg
     1801 caaacggggt attaattaaa gaaatagtat tatcagcaaa aggtcacaat caagtacagt
     1861 tagatgcaga aagtctgcca tcaggcactt tcagctatag cctggtgctt gacggcaagg
     1921 tggtgtcaac aaaaacaatg atttatgtaa aataaaaaac aatataaaac actcaaaaag
     1981 ctacctcctt aagtgaaggt agcttttttt attaaatcac atcatgatgt gatatgctgc
     2041 cggtttgaat ggatatagtt ttaaaccttg aaataaacat caaaaaacaa aatctaaaca
     2101 tcagttaaca tgaaaaatat ccgaacaacc caacccgaaa tcaaacaagt agcattgcag
     2161 caacgattaa aaaatatgtt ggatttaaca attgctaaag tagcagcaca tgttaaggct
     2221 ccacagcaat atgccatgcc tgctgatagt agtgcattag agcataaaat attgaggttt
     2281 tataatgtat tgaaaccgca tgaaaaagaa acatttttag caaaaaccaa tttattattt
     2341 aatcagtcgg ccacagaaaa tagaaaacgt tttgaggatt taatggatat cgatataaaa
     2401 tcatccacac ccatgcttga taaaataaaa gcgtttccac ttccggttaa atatcatttt
     2461 actgaaaagg aggcgactgc aattatagaa aaatcagcaa agcttaaaac tatccaaaaa
     2521 acgaaatcag gccttaccat tgctccgcaa caggctgcag aaccacgaac attgtcctta
     2581 tttttagaat cagttaaatg taacgatccg caggatgtgc ttaaggatga aatttttttt
     2641 tcaggattta cgttggatgc tttaggagta aacgaaccaa ttccatttac atcggcaggg
     2701 aaatacaaaa aaaatgattt taaacaatta caaataaaat tacgcgattt tgatttacgt
     2761 gctgcagggg tttttcctca ggatttttta ttagcgttat ttgtttttga aaaagattct
     2821 aaaaaagatg atgaaaggtt aaaattcacc atggagctga tacaggatat aggattatgg
     2881 gtattggctg ccgccgatgt cattgctatt gcagccttgg ttgtgactat tgctagcact
     2941 tctcctgttg gaatggctct gattattgca tcaggtgtaa ttgctgctat aggtttgtca
     3001 attataggtg tcataagcct gattaataac ttacgcttag ctgaaatttc attacaagta
     3061 acagatttgt ttacctttcc tgaccctcca attaatgtgg gtgaacaggt aattaacaag
     3121 catcaggttg ttttagcagg tagtttaagt ggggttatga aaggcaacta tgatttacaa
     3181 tttcgttggg agcgaacagc ataaattgtg gctaaattag taagattatg aacggtgtta
     3241 tatctttgaa aaaaacacca ctatgaaaaa gtcctatttt atattattgc tttgcggatt
     3301 aagcctgatg tttgccggtt gtccttatgg agccgatgtg ccaattgata aagagccaac
     3361 tattcctatt gataaaacgc tgttaggcaa atgggaaaat agttcagcat cagattacac
     3421 gtattacttc agagcagatg atgccaacac ctatcgaatt tctaaaattt cgagtagcac
     3481 aggtgattca accgtatatt ttgcatttga aagcaaaata gataacgtaa gtttcatcaa
     3541 tatctacgag gaaaatgaat ttggcagtaa aacctactac ttgtataaac tcaccaaacg
     3601 tggaacatct aaaatatcga tggaagaagt taccgacaat attgatgaaa aatttgatac
     3661 cagtgaagaa ttaaaagcct acctcgcgaa acatcagcat ttaagttttc ttttcaacaa
     3721 agatcctgat gaatatgtgc gtatggattg attggtaact atccacacac aaatgatttg
     3781 atttatgtga ggataattat tgtgctatat tcttatactg taaaacttgc agtttaacct
     3841 aatgccaatt ttaatacctc ctattatgcg ttttaatttt taattattgt ttgtccaaat
     3901 aaaaacttat ccgcagcata aaattgaata aaatacaatc ctgtaggcaa aaaggtcaaa
     3961 taaaatgttt catttgattt tgaaatttta tttgtgtaaa caattttacc ggtaatatct
     4021 gtcaagatga aattatcaat tttgacttca ttttcaggta tcactatagc aattacttcc
     4081 gtaaatggat tcgggtaaat agccacttgt gttaattgtt caagtttgca ggcattaata
     4141 atattatacc ctgctgaagt attagagcaa ccgctcgcat tggtaacatt tactttataa
     4201 ttaccaataa ttgttgcatt gaaaatattt gtatttgctc caacaatagg cacatcatcc
     4261 aaataccatt gaaacgtgta accggggcca ttgcttgttt tcagcttaat agaggcatct
     4321 acacacaggt tattggtgcc atctacatta ttaatagata caacaggttt agggttggct
     4381 gttacctgga cttcttgcgc aactttagtg caaataccat tagatatttt aacagtataa
     4441 ttcccggttt tatttgcggt atatatattc gttgttgctc ctgcaatatt cgaaccattg
     4501 cgcctccact ggtaggtaag ggttgcatca tattgagtgg taagtgtaac tgaacctggc
     4561 ttacaaaacg ttgtatcaga agttggtgtt acggctacat gcagtgggca aatgtctgcg
     4621 tacattttat atacccatat atcacttaat ccaataggat tttcattttt ataatcggtt
     4681 gccggcgaat atgaataacc tgctaccacc aaagaaccat catcatttaa gac
//
LOCUS       DAORKW010000264         4701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_817_length_4701_cov_5.430263, whole genome shotgun sequence.
ACCESSION   DAORKW010000264 DAORKW010000000
VERSION     DAORKW010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_817_length_4701_cov_5.430263"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLB46_10495"
     CDS             <1..889
                     /locus_tag="PLB46_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF76991.1"
                     /translation="DGQGRTMERTNPFVAENTPSNWFDGCIGGSPGLPYTPCEDAVIF
                     SEINYHSGADFAADDWIELRNVSTNTIDLSGWKFMDDSIGIAHEYLINDGTSIEPSQH
                     IVLAKNTDAFTGTFPDVSNVIGPFNFELGDNGEWVRMYNADGILTLSVNYRDLAPWPL
                     AADGGNYTLELIDSLGLMNSGLNWETICPGGSPGSYALTPCEGEISINEIELNDFIHI
                     YPNPTESGLYIQMPITEIQQLTVELIAVDGSYTLMYNQTVYPGNFVHYFDIQNFANGV
                     YLLAVQFDGQTRVGTIVKQ"
     gene            1053..2063
                     /gene="hemH"
                     /locus_tag="PLB46_10500"
     CDS             1053..2063
                     /gene="hemH"
                     /locus_tag="PLB46_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRF76992.1"
                     /translation="MQHLKGILLVNLGTPDSPQVPDVRKYLKEFLLDERVIDIPAISR
                     HLLVRGIIAPFRAPRSAKSYKHIWTAEGSPLMVYSVRQQEKLQAALGEEYVVELAMRY
                     QTPSIENGLLALKEKRVNEIIVVPLFPQYTSATNGSIIQKVMEIVAKWQAIPPIRFTG
                     DFHAHPLFIKAFSEIGKKHNPETYDHVLFSYHGLPERQILKTVEGNNCLTTNCCDTLH
                     NRNCFCYRAQCFNTTRLIAEQLQLKPENYTVCFQSRLGKTPWIKPYSTVIIEELAKKG
                     VKRILMYSPAFVADCLETVFEIGVEYHEDFQKMGGEKIQLVESLNEHPLFIDCLIDLI
                     KN"
     gene            complement(2115..4550)
                     /locus_tag="PLB46_10505"
     CDS             complement(2115..4550)
                     /locus_tag="PLB46_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF76993.1"
                     /translation="MKRFAGLLLFYTIFTVQAQMNAINIAGAVADSISKKPIEFAAVT
                     VTLQGDSTIISGGITDMDGKFRFTVNNSGSYTLHVNFIGYVEKHINFNLPAGAPFHNA
                     GMIQMVQDVNLLESVNITAEQSYLQGSIDKKVYNIDKDIVASAGSASDALQTIPSVVI
                     DIDGNISLRGNEGVRIFIDGKPSGIVGTNMNAILEQIPASSIESIEVITNPSARYEAE
                     GNSGIINIVLKRNKKIGLNGNITAGVSTTPKYEGGASLNFRNQKVNVYSNYSYTNDDR
                     DGSGSVFRKTFEEDTTFYLNSLSDNNSKSQMHMARAGMDYYINKQNTIGISGTFHTNT
                     SERLDIVNYTFLDIDSIETSNSIRTTNSNNDGLSYNAGLTYRKTFLNPKQILTADAFY
                     SYGDGNDLSDYNEELFGITDTTLNNLLLQSVSRPSINKDMSIQSDYVHPFKNGNQFET
                     GLKYTKEIKDNTLYSESFDDETNTWMVDDSINNQFVYTEDVIAAYLIWNSSIKKFGYQ
                     VGVRAEQTLTESDLLTTDETYTNNYFGLFPSVHLNYKFTETAELSASYSRRVDRPNAW
                     FLNPFPDYSDPYSFRIGNPYLEPEFENAYDISFIKEFKNHSISASVYYNKTLNEISPY
                     TTVNDEGISYMTFQNYDNEEKYGVELVGKNEFYKWWNVTSSVNFNQTLVDANNLEAGL
                     TNSQFTYNIRVMSFFQVLKQTSFQVTFSYNTPWTFAQGESEPIYFVDAGLKSDFFQNK
                     LSVNVSMSDIFNTRIWEGYSEGINFYSEYSRKRQSQIGTLKLTYKFGQQDNNRRRGSR
                     GMEENYDGGMDMF"
BASE COUNT         1495 a          826 c          815 g         1565 t
ORIGIN      
        1 agatggacaa ggaagaacta tggaacgaac taatcctttt gtggctgaaa acactccttc
       61 aaattggttc gatggttgta ttggcggttc accgggatta ccttataccc cttgtgaaga
      121 tgcagtaata tttagtgaaa taaattatca ttcaggcgct gattttgctg cagacgattg
      181 gattgaatta cgtaatgttt ctacaaatac tattgattta agcggatgga aatttatgga
      241 tgacagtatt ggaattgctc atgaatatct tattaatgac ggaacaagta tcgaaccatc
      301 gcaacatatt gttttagcaa aaaatacaga tgcctttacc ggaactttcc ctgatgtaag
      361 caatgtaatt ggcccattta attttgaact tggtgacaat ggtgaatggg ttagaatgta
      421 taacgcagat ggcattttaa cattatcagt aaattatcgc gacctggcac catggccgtt
      481 agctgctgat ggtggtaact acaccttaga gttaattgat tcgttgggat taatgaacag
      541 tggattaaat tgggaaacaa tttgcccagg cggttcacct ggctcttatg cattaacacc
      601 ttgcgaaggt gaaatatcaa taaatgaaat tgaattaaat gattttattc atatctatcc
      661 caacccaaca gaaagtggtt tgtatatcca aatgcctatt actgaaatac aacaattaac
      721 tgttgaactt atagcagtag atggaagtta tacactaatg tataatcaaa cagtttaccc
      781 tggtaatttt gtccattact ttgatataca aaattttgcc aatggcgtat atctattagc
      841 agtgcaattt gatgggcaaa ctcgggttgg tactattgtt aaacaataat tgtatagcca
      901 tattacgtag cacttattgg tttaatcttg tggtgttggc atctcgatta accttgttca
      961 acatgacttc aaatttaggt taataaaatt gaacaatttt actattcagt tatttatcta
     1021 gttgtaactt tgcgcttcac gaactgcgaa atatgcaaca tttaaaaggt atactgttag
     1081 tcaacctcgg aacacccgac tctcctcagg tgcccgatgt aagaaaatat ttaaaggaat
     1141 ttttgttaga tgaaagggta attgatattc cggcaatttc gcggcattta ttagtacgag
     1201 gcattattgc accattccgc gcacctagga gcgcaaagtc atataagcat atatggactg
     1261 ctgaaggttc tccgttaatg gtatatagtg ttcgtcagca ggaaaaatta caagccgcgt
     1321 tgggcgaaga atatgtagta gaattggcca tgcgttatca aacgccatct attgaaaatg
     1381 gactattggc attaaaggaa aaacgcgtga atgaaattat tgttgtgccg ttatttccac
     1441 aatacacttc tgctaccaat ggctccatta ttcaaaaggt aatggaaatt gtagccaaat
     1501 ggcaggcaat tcctcctatt cggtttacag gtgattttca tgcgcatcct ttattcataa
     1561 aggcttttag tgaaatcggg aaaaaacata accctgaaac ctacgaccat gtattgtttt
     1621 cgtatcatgg attacctgaa cgtcagattt taaaaacagt agaaggtaac aattgtctta
     1681 cgacaaattg ttgtgatacc ctacacaata gaaattgttt ttgttaccgt gcgcaatgtt
     1741 ttaataccac cagattaatt gctgaacaac tacaattaaa accggaaaat tatactgttt
     1801 gctttcaatc tcgtttaggt aaaacacctt ggattaaacc ttattcaaca gtaattatag
     1861 aagagctggc aaaaaaaggt gtaaaacgaa tattaatgta cagtcctgct tttgtggctg
     1921 attgccttga aactgtattt gaaatcggtg tagaatatca cgaagatttt caaaaaatgg
     1981 gcggtgaaaa aatacaatta gttgaaagcc tcaacgagca cccattgttt atcgattgtt
     2041 taatcgacct gattaaaaat taaaaaagcc gggtaataat tattacccgg cctaaataca
     2101 gtttattttt ctatttaaaa catatccatt ccaccatcgt aattttcttc cattcctctg
     2161 ctgcctctgc gacggttatt gtcctgctgt ccaaatttgt aagttaattt taatgtgcca
     2221 atttgggact gacgtttacg ggaatattcg ctataaaagt tgataccttc actatagcct
     2281 tcccaaattc tggtattaaa tatgtcactc atacttacat tcacagataa tttattttga
     2341 aaaaagtcag attttaaacc tgcatcaaca aaataaatag gttctgattc gccctgtgca
     2401 aatgtccaag gtgtattata actgaaggta acttgaaatg aagtttgctt taaaacctgg
     2461 aaaaacgaca ttactctgat attataagta aattgtgaat tcgttaaacc cgcttccaaa
     2521 ttattagcat caacaagtgt ttgattgaaa tttacacttg atgttacatt ccaccattta
     2581 taaaattcat ttttgccaac aagttcaaca ccgtatttct cttcgttatc gtagttctgg
     2641 aaagtcatgt acgaaatccc ttcatcattt acggtggtgt atggtgaaat ttcatttagt
     2701 gttttattat aatatacaga agctgaaata gaatgattct taaactcttt tataaatgaa
     2761 atgtcataag cattttcaaa ttctggttct aaataagggt ttccaattct gaatgaatag
     2821 gggtcagaat aatcaggaaa tggatttaaa aaccaggcat ttggtcgatc aacacgacgg
     2881 ctgtagctgg cagatagttc tgctgtttcg gtaaacttat aatttaagtg aacacttgga
     2941 aataatccaa aataattatt agtataagtt tcgtcggtag taagtaaatc cgattctgtt
     3001 aaagtttgct cggcacgtac acctacctga taaccaaatt ttttaatact gctattccaa
     3061 atcaaataag cagcaataac atcttcagta taaacaaatt ggttattaat tgagtcatca
     3121 accatccacg tattagtttc atcatcaaag gactccgagt ataaggtatt atcttttatt
     3181 tctttcgtat attttaatcc ggtttcaaat tggttgccat ttttaaatgg atgaacgtaa
     3241 tcagattgaa tactcatatc cttattaata gatggacgac taaccgattg cagtaataaa
     3301 ttatttaatg tagtatctgt tataccgaat agttcttcgt tataatcact caaatcatta
     3361 ccatcgccat atgaataaaa tgcatcagca gtgagaattt gttttgggtt taaaaacgtt
     3421 ttgcggtagg ttaaaccggc attatatgat aaaccgtcat tattcgaatt tgtagtcctg
     3481 atactgttgg atgtttcaat actatcaata tctaggaatg tatagttaac aatgtcgagg
     3541 cgctcactgg tatttgtatg aaatgttccg ctaatcccaa ttgtgttttg tttattgata
     3601 taatagtcca ttcctgcgcg tgccatgtgc atttgcgact tactattatt atcacttaag
     3661 gaattgaggt aaaaagtggt atcctcttca aatgtttttc tgaaaacact gccactgcca
     3721 tcgcgatcat cgttggtata actgtaattg gaatacacat taactttttg attcctaaaa
     3781 tttaatgatg caccaccttc atattttggt gtagttgaaa cacctgcagt aatattgccg
     3841 tttagtccaa tttttttatt gcgttttaat actatattga taattccact attgccctct
     3901 gcttcatacc ttgcagatgg attggtaatt acttctatac tctcaataga acttgccgga
     3961 atttgctcca atatggcatt catattcgta cccactatac ctgaaggttt cccatcaata
     4021 aaaatgcgca caccttcatt accacgtaaa cttatattgc catctatatc aataacaacc
     4081 gacggaatag tttgtaaggc atcggatgca gaacccgctg acgctacaat gtccttatca
     4141 atattataca cctttttatc aatacttccc tgtaaataag attgctcggc agtaatgttc
     4201 acactttcca aaagattaac atcctgcacc atttgaatca ttcccgcatt atgaaatgga
     4261 gcacctgctg gcaaattaaa attgatatgt ttttcaacat atccgataaa atttacatgt
     4321 aaggtataac tgccactatt gtttacggta aacctaaatt tcccatccat atctgtaata
     4381 ccaccggaaa tgatggtaga gtcaccttga agtgttactg taactgcagc aaactcaatt
     4441 ggtttttttg aaatggaatc agcaacagca cctgcaatat ttattgcatt catttgtgcc
     4501 tgaaccgtaa aaatggtgta aaacaagagg agccctgcaa atcttttcat cagtatagaa
     4561 tatcgtattt tatatagtgc aaaattaacg catttgactg gtttaactaa gtaaggttta
     4621 gtgcgtttaa gacgtattaa caaatattgt gattttttgt tccaaatgtc acatttcggg
     4681 ttcaaaaccg cattttttat g
//
LOCUS       DAORKW010000265         4694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_819_length_4694_cov_3.778185, whole genome shotgun sequence.
ACCESSION   DAORKW010000265 DAORKW010000000
VERSION     DAORKW010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_819_length_4694_cov_3.778185"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLB46_10510"
     CDS             complement(<1..964)
                     /locus_tag="PLB46_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF76994.1"
                     /translation="MRFYYYIFLFMVVIVSCKQSDTTPANTSTGKTVPVLYAKGFTIT
                     QYDGYKIVVVRDPLDTNKIIQQYRLITDKQFAGNNSNEINIQVPLNYIASLSTTHLGF
                     FEALGVADKLIAFSGTNYIYSDLFKTLVSSGKIQEVGVEGGIDLELLVSLQPDIIMSY
                     QTGDESYDHFEKMKSMQLQPVINNEYLELTPLGQAEWIKFIAAFFNLEEKATAIFDSI
                     ATNYNTIKKQAAGANTKPTVFTGLAYKNEWTIPGGKSFAANFIKDAGATYLWETDDKT
                     GNFALSFEEIFTTAQHADYWLNVSTATMLKEILSIDKRYTYFDPF"
     gene            complement(1018..2304)
                     /locus_tag="PLB46_10515"
     CDS             complement(1018..2304)
                     /locus_tag="PLB46_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF76995.1"
                     /translation="MMRKIFFTFCAVIFTASVFAQAYVERVLILNEGYFDYVTNEIIT
                     PVSVGAYNPTTDTYTVIDEIENARFASDIKMDGDNYYVAADKYLNKYATATDELIASI
                     EIEGIRKVAVNNDYIVVTRGEYLVTLPSYIQIYDKSTMALLDEFDNVTLPYTTEGVVI
                     KDNKAYVAVNNGFVFGSEVGKIAIVDLATTTIENTIELGADGINPDNLMIDGDVIYTL
                     NNKDFTGSSVSTYKIASADLTTTNLINISAGCGTSAVMDGTIYYQEMFGTTLSKFDPS
                     TATIIGEDEFGTSFYGLAFDEINNLIYTGTTDYFSYGEVTVYDLLGNIVTSFETSVSP
                     GNFAFDVRLATGVEDIKSATMELYPNPAVNQIVVVNTINATNMVITDMTGKTVFTVQQ
                     PAAGAQQIDITNLPAGNYIIKAIGQSQYEAAYFSKL"
     regulatory      complement(2396..2607)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2782..3318)
                     /locus_tag="PLB46_10520"
     CDS             complement(2782..3318)
                     /locus_tag="PLB46_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76996.1"
                     /translation="MRNVIICLLILISANTIYAQSPLEDEITQPINLTGPRIGFTYLG
                     NVYTNAINNDPTFDRMYGKETTLYPFVTQFGWQFETRFFTLPNGSCGIIEFVPLLGGL
                     DQNVVIPSLTVMIGARLSNGIEFGMGPNVNLTGSSVSIVGGYTFERMGINFPVNLAVT
                     PTPEGIRGSLLLGFNKHD"
     gene            3537..4532
                     /locus_tag="PLB46_10525"
     CDS             3537..4532
                     /locus_tag="PLB46_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF76997.1"
                     /translation="MENPYQLDFIQRLKLMLPAHISLADQLTEILEVSPDSAYRRIRG
                     ETAMSVDEAIKLCNHYKIPIAYFTDEIPGLVNFKYLPPDGKKETFYKHLQMVYNVSDA
                     LLNHEDRRIIYAAIDAPLFQHFAFEKLAAFKMHFWMRVIDGVEGFRDHYYMEDIDKDF
                     LVLAKKIHETYMVIPSDEIWPEDIMMTTLKQIEYYWDADYFKKKDDALEILASLNDLI
                     DHAENMAALSCKLRPGDKSKQKYAEFNLYQSDVLLGNNTVLTVGDNKITTYISHMTFS
                     ILATNSDLYARQTEAWLKSILSKSNLISGISEKNRAKYFKNLRKFIDATRERIQR"
BASE COUNT         1473 a          811 c          895 g         1515 t
ORIGIN      
        1 tgaatgggtc gaaataagta tatcgtttat caatagataa aatttctttt aacatggttg
       61 ctgtgcttac attcagccaa taatcagcgt gttgtgctgt agtaaatatt tcttcaaacg
      121 acaatgcaaa attgcctgtt ttatcatcgg tttcccataa ataggttgct cctgcatcct
      181 taataaaatt agcagcaaaa cttttacctc cgggaattgt ccattcattt ttgtaggcta
      241 ggccggtgaa cactgttggt tttgtatttg cccctgctgc ttgctttttt atagtattgt
      301 aattggttgc tatactatcg aaaatggctg ttgctttttc ttccagatta aaaaatgccg
      361 caataaattt aatccattct gcctgaccaa gtggtgttaa ctcgaggtat tcgttattga
      421 ttaccggctg caattgcata cttttcattt tttcaaaatg gtcgtacgac tcgtcgcccg
      481 tttggtaact catgatgata tccggttgca ggctgactaa taattctaaa tcaataccgc
      541 cttcaacccc aacctcttgt atttttcctg aggatactaa tgttttaaat aaatcgctgt
      601 aaatataatt tgtcccgcta aatgcaatca atttatccgc tacacccaat gcttcgaaaa
      661 aacctaaatg tgtagtagac aaactggcga tataatttaa agggacttga atgttaattt
      721 catttgaatt attaccggca aattgtttat cggtaattaa acgatattgc tgaataattt
      781 tattggtgtc caaagggtct cgcaccacaa caattttata tccatcgtat tgtgtaatgg
      841 taaatccttt ggcatataac acaggaactg ttttaccagt tgaagtattg gcaggtgttg
      901 tatcagattg tttacatgaa acaatcacaa ccataaacag gaatatgtaa taataaaaac
      961 gcatggaaaa ataaaagcat gttgttactg ttggctaaca acatgcttta gtataattta
     1021 caatttagaa aaatacgcag cttcgtattg cgattgtcca attgctttaa taatataatt
     1081 tcctgcaggc aggttagtta tatcaatttg ttgtgctccg gcagcaggtt gttgaacagt
     1141 aaacacagtt ttaccggtca tatcagtaat caccatattt gttgcattga tggtatttac
     1201 cactacaatt tgatttactg cagggttggg atacaattcc atagttgcac ttttaatatc
     1261 ctcaacaccg gttgctaaac gcacatcaaa agcaaaatta cccggagaaa cacttgtctc
     1321 gaaactggta acaatattgc ctaataaatc ataaaccgta acttcaccat aactaaaata
     1381 atcagtagtt ccggtataaa ttaagttatt aatttcatca aatgctaaac cgtaaaagct
     1441 ggtaccaaat tcatcttcac caataatggt agccgttgaa gggtcgaatt tactgagtgt
     1501 agtaccaaac atttcctgat aatatattgt gccatccatt actgcagaag taccgcatcc
     1561 ggcagaaata ttaatcaggt tggttgtagt taaatctgct gaggcaattt tataagtgga
     1621 aactgaactt cctgtaaaat ctttattatt taaggtgtaa ataacatcac catcaatcat
     1681 caggttatcc ggattaatac catcagcgcc taattcaatc gtattttcga tagttgttgt
     1741 agctaaatcg acaatagcaa ttttacctac ttcgctacca aaaacaaatc cgttgtttac
     1801 cgctacatac gctttattat cttttattac cacaccttct gtggtgtaag gtaatgttac
     1861 attatcaaat tcgtcaagta atgccatcgt gcttttatca tatatctgaa tatatgatgg
     1921 taaggtaacg agatattcgc cgcgtgtaac aacaatataa tcgttgttta cagccacttt
     1981 acgaatacct tcaatttcaa tggaggcaat gagttcatca gttgcagtag catatttgtt
     2041 taaatattta tccgcagcaa cataatagtt gtcaccatcc attttaatat cggatgcaaa
     2101 tcgtgcattt tcaatttcat caataaccgt ataggtatcg gtagttggat tgtaagcgcc
     2161 aacgcttaca ggtgtaataa tttcgttggt cacataatcg aagtaacctt cgttgagaat
     2221 aagcacgcgt tcaacataag cttgtgcaaa aacagatgca gtaaaaatta ccgcgcaaaa
     2281 agtaaaaaaa atcttcctca tcattaaatg atttaaatgg ttaataaata aggaggcatg
     2341 taaataaatg gaaacagtac ggataccggt tacatcaaac ttttgcccca aaagtttttg
     2401 attgtgcagt acgggcaggt cttctggctc ttccaccttt tgatttacct tcccatccac
     2461 caaggcggac agtggcaaag agatatcaaa agtttaccaa caggttgcgg aattacagca
     2521 gtggggactg ccccggattt tcaccggatt ccctcttagg acaaacttga ataatcaggc
     2581 atgtcaacca gtaatgcatg gtaaaaatac aatagggaat tgaggggagg gaattttgtt
     2641 caaataattt atacacaaaa atgccacata ctatactaaa gtatatggca cttgcgtttt
     2701 tgctttgtct tgtcgcagag acgttcaagt aagaaaaggc gctgcgcccc aggggtgcgg
     2761 cgcccttaca tttttatgga cttaatcatg tttattaaac cctagtaata atgagcctct
     2821 tattccttcc ggtgttggtg ttacggctaa gttgaccgga aaattaatcc ccattctttc
     2881 gaaagtataa ccacctacaa ttgatacaga tgagccggta agatttacat taggtcccat
     2941 cccaaattca attccgttgc ttaaccttgc tccaatcata acagttaaac ttggtatcac
     3001 aacattttga tctaaaccac ctaataaagg aacaaattcg ataataccac atgagccgtt
     3061 aggtaaggta aaaaaacgtg tttcaaattg ccatccaaat tgtgtgacaa atggatataa
     3121 ggttgtttct tttccataca tcctatcgaa tgttgggtca ttgttaatgg cattggtata
     3181 aacattacct aaatatgtaa agccgattct tggtcctgtc aggttgatag gttgtgttat
     3241 ttcgtcttct aacggacttt gtgcgtaaat ggtgtttgcg gagatgagga ttagtaaaca
     3301 aataattacg ttgcgcattt ttgttgtttt agtgaaataa tcgatttgat ggtgcaaacg
     3361 tagtgcggta aaaatccggc aattagtaaa aaacagggta agtgttattg gagataatta
     3421 aattaccatt gagttggtag tttcatcctt ttcagttgca ttttttgcaa ctttttgagg
     3481 taaaattgtg attttcaagt atttttcgca aattgcagcc cataaacagc ctaaaaatgg
     3541 agaatcccta ccagttagat tttattcagc gattaaagct gatgcttcct gcacatatct
     3601 cgctcgccga tcagcttaca gaaatattgg aagttagtcc cgacagcgct taccgacgca
     3661 ttagaggaga aacggcaatg agtgtggatg aggctattaa attgtgtaat cattataaaa
     3721 tcccgattgc gtattttact gatgaaattc cgggtttagt caattttaaa tatttaccac
     3781 ctgatggaaa gaaggaaacg ttctacaaac atttacaaat ggtgtataat gtttctgatg
     3841 cgctgttaaa tcacgaggat agaagaataa tttatgcggc aattgatgcg cctttgtttc
     3901 aacattttgc atttgaaaaa ctggctgcat ttaaaatgca tttttggatg cgcgtaattg
     3961 atggagttga aggatttcgc gaccattatt atatggagga tattgataaa gattttttag
     4021 tgttggcgaa aaaaattcat gagacgtata tggtaattcc gagcgatgaa atttggccgg
     4081 aggatatcat gatgactacg ctcaaacaaa ttgaatatta ttgggatgct gattatttta
     4141 aaaagaaaga tgatgcgctg gaaatattgg cttccttaaa tgatttaatc gaccatgcgg
     4201 aaaatatggc tgcattgagt tgcaagttga ggccgggaga taaaagcaaa caaaaatatg
     4261 cggagtttaa tttatatcag tcggatgtgt tgttgggaaa taatactgta ctaacggttg
     4321 gggataataa aattactacc tatatcagtc acatgacgtt tagtatttta gcgacgaata
     4381 gtgatttgta tgcgcggcaa acagaggcct ggttaaaaag tattttgagt aaatcgaatt
     4441 taattagtgg tatctccgaa aaaaatcgtg caaaatattt taaaaattta cgaaaattta
     4501 ttgatgcaac gcgtgagcgg attcaacggt aattgatttt aatttttgtg agtgaatagc
     4561 cgaattcttt aggtcatata gtttgtttat agattgcgtc ggacggagtc ctggcacgat
     4621 gctgcagaat cagagatttt gcagagcagg cactaaagca aattggttta ttgtttaaat
     4681 tttgcgcctt ttgt
//
LOCUS       DAORKW010000266         4648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_835_length_4648_cov_4.948835, whole genome shotgun sequence.
ACCESSION   DAORKW010000266 DAORKW010000000
VERSION     DAORKW010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_835_length_4648_cov_4.948835"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(214..314)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(477..848)
                     /locus_tag="PLB46_10530"
     CDS             complement(477..848)
                     /locus_tag="PLB46_10530"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HRF76998.1"
                     /translation="MNAEIYLWINAFLYAAFALWCMIQPTTTATFSGLSFLNVSGRAE
                     YFAIYVGLEAAWAVMYIVCALNDDLQFAGVFYSVFLYAGVVIGRWISIFQKGVATNNT
                     YIIAILEIILGAWAVVLLSQY"
     gene            898..1569
                     /locus_tag="PLB46_10535"
     CDS             898..1569
                     /locus_tag="PLB46_10535"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRF76999.1"
                     /translation="MEKLPILYEDKHLMLVNKPAGIAVERDRYRNPSVEGRIGDYFNA
                     QKLPHNTIIGIAHRLDRPVSGVMLVAKKKSVLVQINQQFEAGLIKKIYFAITENKPEK
                     LKATLNHFLFKDVKNKKAVVYDKIAKSRLPCSLDYIYKGENAFGHLIEVHPHTGKFHQ
                     IRAQLAFIGCPIIGDTKYGAKKTFAENEIGLHAFSLQFFHPVEQKEMIIQAPLLPTWE
                     VNTEV"
     gene            complement(1579..3732)
                     /gene="rnr"
                     /locus_tag="PLB46_10540"
     CDS             complement(1579..3732)
                     /gene="rnr"
                     /locus_tag="PLB46_10540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRF77000.1"
                     /translation="MSKKKKKQNTRLVKAGRDFATELYSFLIDKSAKGASLKVIFKWA
                     GKTGQLDGVFDAIENLVAEGKIEEYAPDIFRAKQKTAAINTMIGIVDMTQSGSAYVII
                     DGHLYDVYVPSHKTNRAFNGDTVRVKITALGKKKKPDGEIVEILKRGKELFIGKVDIN
                     DKFAFGITDDRFNNYDFYIPNEEIKKHKIVSGDRVIMRIKDWPPTMKNPIAQIVSKLS
                     QPGNHSSDMEAILVETGINYIFPKEVEQQAEELPTEITKAEIKRRRDMRDIFTITIDP
                     YDAKDFDDAISYRKLENGFIEIGVHIADVSHYIIPDGPIDKEAYSRGTSVYLVDRVIP
                     MLPEKLSNNVCSLRPHEEKLTFSAIFEMDEKGHIVNEWFGKTVIYSDRRFTYEEVQEI
                     IESKSGEYVDEILAINKISTLLREKRFASGAISFETQEVKFILDENFVPTGLYVKERK
                     EAHMLIEDLMLLANRRVAFHLSKNFTKVPFVYRVHDTPDIQKLEEFGENAKRFGHKLK
                     LDSPKNISAELNRLMNDIKGKPEQNILESLAIRCMAKAVYTTKNIGHYGLAFEHYTHF
                     TSPIRRYPDLMVHRILANVLDNHTLLYANREQLEDKCRHSSATERKAMEAERESVKYK
                     QVEYMTKHVGESFDGVISGITSWGVFVEIVENKCEGMIHVDNIRDELMYDEARRRLVS
                     LTNSTNYELGNKIRIKVQKADLATRKLEFIPDSIE"
     gene            3789..>4648
                     /locus_tag="PLB46_10545"
     CDS             3789..>4648
                     /locus_tag="PLB46_10545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF77001.1"
                     /translation="MRNLKPYLIILIAGSMLFFPFLGKSHLFDWDEINFAETSREMVL
                     TGNYTQAQVDYQQFWEKPPLFFWLQSSAMRIFCINEFAARFPNAVCGILTLMLLFAIG
                     KKLKDEKFAWLWVLTYAGSILPQFYFKSGIIDPWFNLFMFIGIYAFAEYAQNKFRLKW
                     LFISGLAAGLAALTKGPVGIGIVGLTIGIYYIVNRFRNFPPILHIVYYVFFIGLSILP
                     WVVIEVAQNGTWFLSEFFKYQLRLATTEDASHGGFFGYHFVVVFFLCFPVSAFAIRPL
                     FQKTDASTNYL"
BASE COUNT         1393 a          872 c          820 g         1563 t
ORIGIN      
        1 ggcatgaata attgttgttt ctttttgcat aataaagtgt tttttgattt gaaaaaattg
       61 tttcaatcag aaaacactta tcaaaattaa tttctaccgg aaagccctat gcataaaggt
      121 tgtagcctac aaattaattg tatggttcac cgatgggcat aaaaaaagct caccgataaa
      181 ttggcgagct taaatatctt tttttatctc tccaacttat ctgtctgaaa ttagcacctt
      241 cacaacaacg agttaaattg ttatgggttg ccaaggtatc gtagggtcag tccctccacc
      301 tttcttgata agtaatttct aaagaacgtg atgcaaagat ataatcagca aactgacaaa
      361 tgcaaattta ttttatgaaa tttcaaaagt tacactagtc tgacactaaa aaagtattta
      421 ctaaaccgga taacttgata agctgtaagc agcaataatt ctgagttttg agtgtattag
      481 tattgcgata ataatactac tgcccatgca cctaaaataa tttcaagtat tgctatgatg
      541 taggtattat tagtagcaac tcctttttga aaaatggaaa tccacctgcc aataaccact
      601 ccggcatata aaaaaacgga atagaataca cctgcaaatt gcaaatcatc gttgagggca
      661 caaactatat acataactgc ccatgctgcc tcgagcccaa cataaatcgc aaaatattca
      721 gctcgtcccg aaacatttaa aaaagacagt cctgaaaatg ttgccgtagt ggttggctgt
      781 atcatacacc aaagggcaaa tgcggcgtat aaaaaagcat tgatccataa atatatttct
      841 gcattcatgc tataaaattg gttttagacc ttaaatatag gttatttttg gacgcagatg
      901 gaaaaactac ccatattata cgaggacaaa catttaatgc tggtgaacaa gccggcaggt
      961 atagccgtag aacgcgaccg ttaccggaat ccaagtgtgg aaggtcgaat tggcgactat
     1021 tttaatgccc agaaacttcc gcataatacg atcataggta ttgcacatcg cctcgacagg
     1081 cctgtaagtg gagttatgtt ggttgcgaaa aagaaatcgg tgctggtgca aattaatcag
     1141 caatttgaag cagggttaat taaaaagata tactttgcca ttactgaaaa taaacctgaa
     1201 aaattaaaag ccacattaaa ccacttttta tttaaggatg taaaaaataa aaaagcggtt
     1261 gtttacgata aaattgcgaa aagcagattg ccttgctcct tagattatat ttacaaaggt
     1321 gaaaatgctt tcgggcattt aattgaagtg catccgcata ccggaaaatt tcatcaaata
     1381 cgcgcacaac ttgcatttat cggatgtccg attattggtg atacgaagta tggggctaaa
     1441 aaaacatttg cagaaaatga aattggcctg catgcttttt cgctccagtt ttttcatcct
     1501 gtagaacaaa aagaaatgat tattcaggct cccttattac caacctggga agtaaataca
     1561 gaagtataac caataaaatt attctatact atcaggaata aattcaagtt tcctggttgc
     1621 caaatctgct ttttgaactt taatccgaat tttattaccc aattcataat ttgtcgaatt
     1681 agttaagctt actaatctgc gacgagcttc atcgtacatt aattcatcgc gaatattatc
     1741 cacgtgaatc ataccttcgc atttattttc tactatttcg acaaaaacgc cccagctagt
     1801 aattcccgaa attacaccat caaaactttc accaacatgt ttggtcatat attcaacttg
     1861 tttgtatttc acactttcac gctcagcttc cattgccttg cgttcagttg cagatgagtg
     1921 acggcattta tcttccagct gctctctgtt ggcatataat aatgtatggt tatccaacac
     1981 attggctaat atgcgatgca ccattaaatc cggatacctg cggatagggg aggtgaaatg
     2041 tgtatagtgt tcaaaagcga ggccatagtg accaatattt tttgttgtat atactgcctt
     2101 tgccatacaa cgaatggcta aactttctaa aatattttgc tcaggtttgc cctttatatc
     2161 attcatcaaa cgattaagtt ctgcagatat atttttgggt gaatccaatt ttaatttgtg
     2221 cccaaaccgc tttgcgtttt ctccaaactc ttccaatttt tgaatatcag gtgtgtcgtg
     2281 cacacgatat acaaacggaa cttttgtgaa atttttcgat aaatgaaatg ctactcttcg
     2341 gttagctaat aacatgaggt cctctatcag catgtgggct tcttttcttt ccttcacata
     2401 taaacctgtc gggacaaaat tttcatctaa aataaatttc acttcttgcg tttcgaatga
     2461 aattgcacca ctggcaaatc ttttttcgcg taataaagtg gagattttat tgatagctaa
     2521 aatttcatct acatattcgc ctgatttact ttcaataatt tcctgtactt cttcataggt
     2581 gaatcgcctg tccgaataaa ttactgtttt accaaaccat tcgtttacaa tatgaccttt
     2641 ttcatccatt tcaaaaatgg cactaaaggt taatttctcc tcgtgtggac gcaacgaaca
     2701 tacattgtta gatagttttt caggtaacat aggtataacc ctgtcaacta aataaacaga
     2761 ggtaccgcgt gaataagctt ctttatcaat aggtccgtct ggaataatat aatgtgacac
     2821 atctgcaata tgtacaccta tttcgataaa tccattttcc agttttctat atgaaattgc
     2881 atcatcaaaa tctttagcat cataagggtc gatggtgatg gtgaaaatat cgcgcatatc
     2941 gcgacggcgt ttaatttcag ccttggttat ttctgttgga agttcttctg cctgttgctc
     3001 aacttctttc ggaaaaatat aattgatacc ggtttccact aaaatagctt ccatatcgct
     3061 ggagtgatta ccgggttgtg aaagtttact gacaatttga gcaatagggt ttttcatagt
     3121 tggcggccag tcttttatgc gcataattac gcggtcgcca ctaactattt tatgtttttt
     3181 tatttcttcg ttgggaatgt aaaaatcata attattaaat cggtcatctg taataccaaa
     3241 tgcgaattta tcgttgatat caactttacc aataaataat tctttgccgc gtttgagaat
     3301 ttctacgatt tctccgtccg gttttttctt tttacccagt gcagtaattt tcacgcgaac
     3361 agtatcaccg ttaaatgcac gattggtttt gtgtgaggga acataaacat catataaatg
     3421 cccgtcgata attacgtaag cacttccgct ttgggtcata tcaacaatac caatcatggt
     3481 atttattgcc gctgtttttt gtttggctct gaagatatca ggtgcatact cctcaatttt
     3541 cccttcagcg actaaatttt caattgcgtc aaaaacgcca tctaactgcc cggttttgcc
     3601 ggcccattta aaaatcactt ttaacgatgc cccttttgcc gatttatcaa tcagaaagga
     3661 atataactca gtcgcaaagt cgcgccctgc tttaactaat cgcgtatttt gcttcttttt
     3721 tttctttgac atatggtaaa gatacactta tgccacgaaa tcatttcgta attttccacc
     3781 gtcaaagtat gcgtaatctg aaaccgtatc tcattatcct gattgcagga agcatgttat
     3841 ttttcccgtt tttaggaaaa tcacacttat ttgattggga tgaaataaat tttgctgaaa
     3901 catcgcgtga aatggtgctc accggcaatt atactcaggc tcaggtggat tatcagcagt
     3961 tttgggaaaa accaccctta tttttctggt tgcaaagttc agcaatgcgc attttttgta
     4021 ttaatgaatt tgctgcgcga ttccccaatg cggtttgtgg tattttgacc ttaatgttat
     4081 tgttcgccat tggcaaaaag ttaaaagatg aaaaatttgc ctggctttgg gtgttaactt
     4141 atgcaggcag tatattacca cagttttatt ttaaatcagg aattattgat ccctggttta
     4201 atttattcat gtttataggt atttatgctt tcgctgaata tgctcaaaat aaattccgac
     4261 taaaatggtt atttatttcg ggcctggctg ccggtttagc ggcacttaca aaaggtcctg
     4321 ttggtattgg tattgtgggg ctgactatcg gaatttatta tatcgtaaat agattccgga
     4381 attttcctcc aatattacat attgtatact acgttttttt tattgggtta agtatattgc
     4441 cctgggtggt aattgaagtt gctcaaaatg ggacttggtt tttatctgaa ttttttaaat
     4501 accaactgcg cttggcaact actgaggatg ccagtcatgg agggtttttt ggttatcatt
     4561 ttgtcgtggt attcttttta tgttttccgg tgtcagcttt tgcaatcagg ccattatttc
     4621 aaaaaacgga tgcgtctacc aattacct
//
LOCUS       DAORKW010000267         4636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_840_length_4636_cov_5.129229, whole genome shotgun sequence.
ACCESSION   DAORKW010000267 DAORKW010000000
VERSION     DAORKW010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_840_length_4636_cov_5.129229"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLB46_10550"
     CDS             <1..546
                     /locus_tag="PLB46_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77002.1"
                     /translation="YIKTYSQNNKVEEKVEEIIAEIINPVQEIQKTVIEEEVIEETPV
                     FELKKQIEEPIEIVAEEEEEIITFIKKEEIPPVDEVEEVPPMVVRNEPKSFITNEDKM
                     GVVNNQGNDRINRLKSMSMKLNNNNLEEIEKVPAYMRRNIELEETPDAREINVSQYNV
                     VNGDNGPELKKNNSFLHDNVD"
     gene            complement(673..1533)
                     /locus_tag="PLB46_10555"
     CDS             complement(673..1533)
                     /locus_tag="PLB46_10555"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF77003.1"
                     /translation="MNTNWLNTNEYPFKHHYFNVNDTTMHYVDEGEGEVILFVHGTPS
                     WSFEFRNVIKFLSKKYRCIALDHIGFGLSEKPAKYDYTVQNHTASLLKLITHLQLNQF
                     TMLVHDFGGIIGLAAAEQIPEKIKRLIILNTWCRSIQDEPEYKKMKVILGSPLMPLLY
                     RYLNFSAKYILPAAFGERSRLTPEIHQHFLRPFSKASERNGTIAFAKSLLRDQDYYAS
                     IGKKLTILKDKPVLIIWGMKDEFITEKHLLWMQEQFPGSKVVRYDDAGHFVLEEKSVV
                     AGPVIAEFMG"
     gene            complement(1530..2048)
                     /locus_tag="PLB46_10560"
     CDS             complement(1530..2048)
                     /locus_tag="PLB46_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF77004.1"
                     /translation="METALQLFAGKGYGQTSIALIAREAGVAQGLMYNYFDSKEALLL
                     AIMEQGFAGVQESMAVYAGTHSPKKALELHVEATFAQVTANKNFWKLFHAIKMQEQVQ
                     QFLDAEYAHAKSFIIKTLAGNFKKLGYDKPTDEAKLFFVMIDGLVVHHLMDAKTFPLN
                     TIKKTIFQRYHL"
     gene            complement(2166..3209)
                     /locus_tag="PLB46_10565"
     CDS             complement(2166..3209)
                     /locus_tag="PLB46_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HRF77005.1"
                     /translation="MPKINIYSRETIIARTKHRSGETKLGETIHTIPQNTSWQTYLKQ
                     DTAKFVVLGIPEDIGVRANHGRGGAYAAWKPSLDFLLNMQSNQFCHGEEILVLGHIEL
                     EDLMAEANALNYKLETDIEKARLLVAQVDERVSEVITIIAAAGKIPIVVGGGHNNAYP
                     IIKGINTGAELNGIHVINCDPHSDMRPMLGRHSGNGFLYAYSEGYLKKYAVFGLHESY
                     NIYSALELFEQHKEQLHFETYDDIFVREHISYQLALNNCIHFCKGAACGVEIDLDSIQ
                     NVPVSARTPSGMLPIDVRKYVYHCGSELAVCYLHIAEGAPVLAHKQMDNKTGKLVAYI
                     ITDFVKAVKRNIH"
     gene            complement(3312..>4636)
                     /gene="lpdA"
                     /locus_tag="PLB46_10570"
     CDS             complement(3312..>4636)
                     /gene="lpdA"
                     /locus_tag="PLB46_10570"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRF77006.1"
                     /translation="QLGLKTAIVERAELGGICLNWGCIPTKALLKSAQVFEYMKHAAD
                     YGITVSDAKADFAGMVKRSRDVAGGMSKGVQFLMKKNKIDIIEGNGVVKPGKAVEVTD
                     ATGAKKMVSAKHIIIATGARSKDLPTMKQDGKKIIGYREAMSLATQPKKLVVVGAGAI
                     GVEFAYFYNSIGTQVTIVEFMDRLVPVEDEEVSKELARNFKKQGITIMTSSAVESVDT
                     SGEGCKVKVKTAKGEEILECDIVLSAAGVVANIENIGLETVGIKTDKGKIVVDKYYKT
                     NVDGYYAIGDVTPGPALAHVASAEGITCVEAIAGHHPEGIDYGNIPGCTYCSPEIASV
                     GMTEAAAKAAGYELKIGKFPFTASGKAKAGGNAEGFIKVIFDAKYGEFLGAHMIGANV
                     TEMIAEVVVARKLETTGHEIIKAIHPHPTMSEAIMEAAAAAYGEVIHL"
BASE COUNT         1436 a         1003 c          746 g         1451 t
ORIGIN      
        1 tatattaaaa cttacagtca aaataataaa gtagaagaaa aagtagaaga aataattgct
       61 gaaattatta atcctgttca ggaaattcaa aaaacggtaa ttgaagagga agtaattgaa
      121 gaaactcctg tttttgaatt aaaaaaacaa atcgaagaac cgattgaaat cgtagctgaa
      181 gaagaggagg aaataatcac gtttataaaa aaggaagaaa tacctccggt agatgaagtt
      241 gaagaggttc ctccaatggt tgtgcgtaat gaacctaaat cgtttatcac caatgaagat
      301 aaaatgggtg tggtaaacaa tcagggcaac gaccgtatta atcgtttaaa aagcatgagc
      361 atgaaattga ataataataa tctcgaagag atagaaaaag tgcctgctta tatgcgtcgt
      421 aatattgaat tggaagaaac accggatgca cgtgaaatta atgtatcaca atataatgtg
      481 gtaaatggtg acaatggtcc ggagcttaaa aagaacaatt catttttgca cgataacgta
      541 gattaattcg ccaattaata taatacaaaa gcgcttcgaa agaagcgctt tttttgtttt
      601 aattagttat tgaaattcga tttttcggtt aatatgtcag gttgctctaa aacctggcgg
      661 gtctcgtttt cctcacccca taaattccgc aatcacaggc ccggcaacaa cacttttttc
      721 ttctaataca aaatgacctg catcatcata acgcactact ttcgagcccg gaaattgttc
      781 ttgcatccaa agcagatgtt tttctgttat aaattcgtcc ttcatacccc agataattaa
      841 taccggttta tcttttagga tggttaattt tttgccaatg cttgcataat aatcctggtc
      901 gcgcaacaac gatttcgcaa atgcaattgt cccgttgcgt tcactcgctt tggaaaaagg
      961 acgcagaaag tgttggtgaa tttcaggcgt taaacgtgaa cgttccccaa atgctgcagg
     1021 taaaatatat tttgctgaaa aatttaaata tcgatataac aatggcatta acgggctgcc
     1081 taaaataact ttcatttttt tatactccgg ttcatcctgt atcgacctgc accaggtatt
     1141 taatattatt aaacgtttaa ttttttccgg tatttgctcc gcggcggcta atccaataat
     1201 accaccaaaa tcgtgaacca acatggtaaa ttgatttaac tgcaaatggg taatcagctt
     1261 gagcaaactc gccgtatgat tttgaacggt ataatcgtac ttagcaggtt tttccgataa
     1321 gccaaaacca atatggtcta atgcaatgca tctatatttt ttcgataaaa atttaatcac
     1381 attcctgaac tcaaaactcc aggatggggt tccatgcaca aacaatatca cttctccttc
     1441 accctcatcc acataatgca tcgttgtatc attaacattg aaataatgat gtttaaatgg
     1501 gtattcattt gtatttagcc aattcgtatt cataaatgat atctttgaaa tattgttttt
     1561 ttaattgtat ttagcggaaa tgttttcgca tccattaaat gatgcacaac caaaccatct
     1621 atcatgacaa aaaataattt cgcctcatcg gtgggtttgt cgtaaccgag ttttttaaaa
     1681 ttaccggcta aagttttaat aataaatgat tttgcatggg catattccgc atccaaaaac
     1741 tgctgcacct gttcctgcat tttaatagcg tgaaacaact tccagaaatt tttgttggcg
     1801 gtcacttgtg cgaaagttgc ttccacatgt aattccaagg cttttttcgg actgtgcgtt
     1861 ccggcatata cagccatact ttcctgcaca ccggcaaaac cttgctccat aattgctaat
     1921 aataatgcct ctttggagtc gaaataatta tacatcaacc cctgcgcaac tcccgcctcg
     1981 cgggcaatca acgctatgga agtttgtccg taacccttgc cggcaaacaa ctgcagggcc
     2041 gtttccatga tatgttcttt ctgatttgtt ttcattatga atgaacgttc attcacaaag
     2101 gtagaaacac cggattaatc caccaaattt ttctttaaaa taataaatgt gtaaagctat
     2161 tggacttagt ggatattgcg ttttaccgct tttacaaaat ctgtaatgat atacgcgacc
     2221 agttttccgg ttttgttgtc catctgtttg tgtgccaata ccggcgctcc ttccgcaata
     2281 tgtaaataac aaacagctaa ttccgaacca cagtgataca cgtatttgcg cacatcaata
     2341 ggtaacatgc cacttggagt tcgggcactt actggtacat tttgtatgga atctaaatca
     2401 atttcaaccc cgcatgcagc acctttacaa aaatgaatgc aattgtttag tgccagctga
     2461 taactgatat gttcgcgtac aaaaatatca tcatacgttt caaaatgtaa ttgctcttta
     2521 tgttgctcaa atagttcaag tgcggaataa atattatacg attcatgtaa tccaaatacc
     2581 gcatacttct ttaaataccc ttcgctatat gcatataaaa atccattacc gctatgtctg
     2641 cccaacatcg gacgcatatc actatgtggg tcgcaattaa ttacatgaat gccatttaac
     2701 tcagcgcctg tatttattcc tttaataata ggatacgcat tattatgtcc accaccaaca
     2761 acaattggaa ttttacctgc tgctgcaatt atggttatta cttcactcac ccgttcatcc
     2821 acttgcgcaa ctaataaacg cgctttttca atatctgttt ccagtttata attcagcgca
     2881 ttggcttctg ccattaaatc ttctaattca atatgcccta gcacgagtat ttcttcacca
     2941 tgacaaaact gattactctg catattcaac aaaaaatcta aactcggttt ccatgctgcg
     3001 taagcgccac cgcgaccgtg atttgcgcga acaccgatat cttccggaat gcccaacaca
     3061 acaaacttgg ctgtatcctg ttttaaataa gtttgccatg atgtgttctg cggaatagta
     3121 tgaatagttt cacccaattt tgtctcaccc gagcgatgtt tcgtgcgtgc aataattgtc
     3181 tcccgtgaat aaatatttat tttcggcata ttacaaatgt aattaaatct tctacaaaaa
     3241 aaaggactgc aataaaatca aaaaagcccc gtctcacaac agggctttta ataatatgtt
     3301 atacataata attataaatg tatcacttca ccatacgcag cagcagcagc ttccataatg
     3361 gcttcgctca tagttggatg cgggtgaata gctttaatta tttcgtgacc ggtagtttct
     3421 aatttacgtg caacaactac ttccgcaatc atttccgtaa cgttggcacc aatcatatgc
     3481 gcacctaaaa actcaccgta tttagcatca aaaattactt taataaatcc ttctgcatta
     3541 ccaccagctt ttgcttttcc gcttgcagtg aaaggaaatt taccaatttt taattcgtaa
     3601 cccgcagctt ttgcagcagc ttctgtcata ccaacactcg caatttcagg agagcaataa
     3661 gtacaacccg gaatattacc ataatcaatg ccttccggat gatgacctgc aatagcttca
     3721 acacaagtaa taccttcagc acttgccacg tgtgctaatg ccggaccggg agttacatcg
     3781 ccaatagcat aatagccatc cacatttgtt ttgtaatatt tatctactac aattttgcct
     3841 ttatctgttt taataccaac tgtttccaat ccgatatttt caatatttgc aacaacacct
     3901 gcagcgctta atacgatatc acattctaaa atctcttcac cttttgcagt tttcactttc
     3961 actttacaac cttcgccaga agtatccacc gattcaacag cactcgatgt cataatagtg
     4021 ataccttgtt ttttgaaatt acgcgctaat tcttttgata cctcttcatc ttcaacagga
     4081 actaatctat ccataaattc aacaattgta acttgagtgc caattgaatt atagaaatac
     4141 gcaaactcaa caccaattgc acctgctcca acaacaacta attttttagg ttgagtagcc
     4201 aatgacattg cttcgcgata accaattatt tttttaccat cctgtttcat agtaggtaaa
     4261 tctttactgc gcgcacccgt agcaataatg atatgttttg ccgacaccat ttttttagca
     4321 ccggtagcat ctgtcacttc aacagctttt cccggtttca ctacaccatt tccttcaata
     4381 atgtcgattt tatttttttt catcaaaaac tgcacacctt tactcatgcc accggcaacg
     4441 tcgcgactgc gttttaccat gcccgcaaaa tccgctttgg catcagaaac cgtaattccg
     4501 tagtcggctg cgtgtttcat atattcaaac acctgcgcac tttttaacaa tgctttggta
     4561 ggtatacaac cccagttcaa acaaatacca cccagttcag cgcgctccac aatggcagtc
     4621 tttaaaccca actgag
//
LOCUS       DAORKW010000268         4620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_849_length_4620_cov_5.671194, whole genome shotgun sequence.
ACCESSION   DAORKW010000268 DAORKW010000000
VERSION     DAORKW010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_849_length_4620_cov_5.671194"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLB46_10575"
     CDS             complement(<1..276)
                     /locus_tag="PLB46_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HRF77007.1"
                     /translation="MLRIDPASTPMPKMHQYMLGSISPRPIAFASTIDADGNMNLAPF
                     SFFNAIGSNPPMVIFSPARSGRDNTTKHTLDNADAMKEVVINIVNYPI"
     gene            complement(286..1560)
                     /gene="fahA"
                     /locus_tag="PLB46_10580"
     CDS             complement(286..1560)
                     /gene="fahA"
                     /locus_tag="PLB46_10580"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HRF77008.1"
                     /translation="MIEVNDPKLTSWVPVEAGSDFPIQNLPFGVFQIDDHHPRVGVAI
                     GNFVLDLYALQQDDYFGALLIPDEAFRSYTLNALIKTGKFKIRALRFAISMLLRSDNE
                     TLKNSANIYGKYLIPMEDVKMLLPIEPGDYTDFYSSIQHATNIGKMFRPDNPLLPNWK
                     HMPIGYHGRCSSIVPTGTEIRRPKGQIIYKDGDAPQFKATEQLDFELEMAFITHDGKM
                     LGESITTEEADDFIFGMVIFNDWSARDIQRWEYVPLGPFLSKNFASSISPWVVTLDAL
                     EPFRIPGEKQEPEVLDYLKFKGDKNIDINLAVYIQPEFAQPTLVSSSNYKYMYWNMAQ
                     QLAHQTINGCNVRAADIYASGTISGNEPNSYGSMMELTWRGANPLTMSDGSERKFIND
                     FDTVIIKAWCEKNGVRIGFGEVRNSILPALKK"
     gene            1628..2110
                     /locus_tag="PLB46_10585"
     CDS             1628..2110
                     /locus_tag="PLB46_10585"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRF77009.1"
                     /translation="MDIFLLILAIICMLVGLAGSFLPILPGPPICFLGFILVEITAYA
                     DFTTSFLIVWAILVVILGILEYYIPTWGTKKYGGTKSGQKGATIGTILGIFIPPQPLG
                     LILGPFVGAYIGEIMHDKYDKRKALRSAWGSFIGFLAGTFLKVVTVLVMAFFFIRELV
                     "
     gene            complement(2107..3558)
                     /locus_tag="PLB46_10590"
     CDS             complement(2107..3558)
                     /locus_tag="PLB46_10590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF77010.1"
                     /translation="MLSNISKRPLFFLLTVAAILQTVLIVFYHFNGFFGQDSYEYLRI
                     TNSLTAFYNDGTAPAYTVYPIMYPIVCSIADFFFPSTMFAMQFVSMISMLIAAYLLVK
                     IIKLITTDAHSAPLFVLMFFVLSPYILRFSLIAMSDMFCITMWLFTLYYSLRFEQQAK
                     LKYLLLSSFFAGITLMTRYPSIILLLLPAFICLKTIFTTKQYLFILPAAIVALLSTIP
                     DLIIRQRFIFWNIGNEGPAFSYNFFADQFSITNLFKSSFFNIDGWQHYPTPNIVFGFF
                     QFIHPAFIFCGILFLILLFKQQNRHRYTFPIMFTIMLYSLFIGCNPYQNNRYLMFVVP
                     AVLLVYYLPFTNIIRSLKQKPKLINLLFSGIVICQLLLFTISFQKIVFYNKLEHSIAD
                     HVGNLDKLPIYTLSIDGALLAYNPNMEIINLFNTELSAGDVQSGYLLYNPNAFSKQFE
                     GLLPEKNYHFLQLNTRLKKITAFEDGWELFQIN"
     gene            complement(3586..3942)
                     /locus_tag="PLB46_10595"
     CDS             complement(3586..3942)
                     /locus_tag="PLB46_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77011.1"
                     /translation="MTKTENWMHNSLSEIAEMCDLTVEEQMLEVTSADAFKNSLAEMI
                     AFLIDHNFEKLLWILYRIDVDEEKAKSLLGKHLPEDAPTVLADLIYQRQLKKEELKQQ
                     FENTAKLYEDDDDLRL"
     gene            complement(3973..>4620)
                     /locus_tag="PLB46_10600"
     CDS             complement(3973..>4620)
                     /locus_tag="PLB46_10600"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HRF77012.1"
                     /translation="WTAMLSWGQRTTIINNANTLIALMDNAPYDFIVGHTEQDVKRFA
                     HFKHRTFNYTDTLYFLHFFQQFYNAHDSLEAAFLFKGVEKEKTVEKMLIQFHNSFFND
                     EHAPQRTRKHIATPATKSACKRINMFLRWMVRPNTTGVDFGCWKNIGTHQLICPLDVH
                     VERVAKSLGLLERKQTDWQAALELTENLRLFDKNDPVKYDFALFGAGVMEKWHDI"
BASE COUNT         1483 a          842 c          823 g         1472 t
ORIGIN      
        1 aatgggataa ttcacaatat taattaccac ttctttcatc gcatctgcat tgtccaaagt
       61 atgtttagtt gtattgtcgc ggccacttct tgctggcgaa aaaattacca tcggcggatt
      121 gctgcctatt gcattaaaaa aactaaatgg tgctaaattc atgttgccat ctgcatcaat
      181 agtacttgca aaagcaatag ggcggggcga tatactcccc aacatatatt ggtgcatttt
      241 tggcatcgga gtcgaagccg ggtctattct taacatagta aaattttatt tttttaatgc
      301 cggtaaaatg gaattgcgta cttcaccaaa tccaattcgt acaccatttt tttcacacca
      361 tgcttttatg ataacagtat caaagtcgtt gataaattta cgttcacttc catcgctcat
      421 ggttaatgga tttgcacctc gccatgtcaa ttccatcatg ctgccatatg aatttggttc
      481 attgccacta atcgttccgg aagcataaat atctgcagca cgcacattac aaccattaat
      541 tgtttgatgc gctaactgct gcgccatatt ccaatacata tatttatagt tggaactgga
      601 tactaatgta ggttgtgcaa attccggctg aatatatact gctaaattaa tatcaatgtt
      661 tttatcacct ttaaatttta aataatctaa tacctcaggt tcttgttttt cgcccggaat
      721 tctaaatggc tccaatgcat ctaatgtaac tacccaaggt gaaattgaag aggcaaagtt
      781 tttagataaa aatggcccga gtggaacata ttcccaacgt tgaatatcgc gtgcactcca
      841 gtcgttaaat atcaccatgc caaaaataaa atcatctgct tcttcggtag tgatgctttc
      901 tcccaacatt tttccatcat gtgtgataaa tgccatctcc aattcaaaat ctaattgttc
      961 agtagcttta aattgcggcg catcaccatc tttataaatg atttgtcctt ttggtctgcg
     1021 aatttcagtt cctgttggta caatagatga acatctgccg tgataaccaa ttggcatatg
     1081 tttccagttt ggtaataatg gattatcagg gcgaaacatt ttgccaatat tggtagcatg
     1141 ctgaatgctg gaataaaaat cggtgtaatc gccaggttct attggtagca acattttaac
     1201 gtcttccatc ggtattaaat atttgccgta aatgttggcg ctgtttttta atgtttcatt
     1261 atcgctgcgc aaaagcattg agatggcaaa tcgaagggca cgtattttaa atttaccggt
     1321 tttaattaaa gcgtttaagg tataactgcg aaatgcttca tccggaatta ataaagcacc
     1381 aaaataatcg tcttgctgta atgcatacaa atcgagcaca aaattgccaa tggctacccc
     1441 aacacgtgga tggtgatcat ctatttgaaa taccccaaaa ggcaggtttt gtatcgggaa
     1501 gtcacttccg gcttcaaccg gcacccagga tgttaatttt gggtcattta cctcaatcat
     1561 ctttatttca atttattttt gcaaaaatac aacaacaggg cttttcgtct gttgatataa
     1621 ataactaatg gatatttttt tactaatact cgcaattata tgcatgctgg ttggtttggc
     1681 tggtagtttt ctgcccattt tacccggtcc tcctatctgt tttctaggat ttatcttggt
     1741 agaaataact gcatatgccg acttcacaac tagttttcta atagtctggg caattctggt
     1801 ggttattttg ggaattttgg agtattacat tccaacctgg ggaacgaaaa agtatggtgg
     1861 aacaaaatcc ggacaaaaag gagcaactat agggacaatc ctgggcattt ttatcccgcc
     1921 acaaccgctt ggtcttattc ttggcccttt tgtgggcgca tatatcggag aaataatgca
     1981 cgataaatac gataaacgca aagcccttag aagcgcatgg ggaagtttta ttggcttttt
     2041 agcaggaaca tttttaaaag tagttactgt tttggtgatg gcatttttct ttataagaga
     2101 attagtctaa ttaatttgaa ataattccca tccatcttca aatgctgtta ttttcttcaa
     2161 cctggtattc aattgtaaaa agtgataatt tttttcaggc aataatcctt caaactgttt
     2221 tgaaaatgca tttggattat acaacaaata acctgattgc acatcacctg ctgaaagttc
     2281 cgtgttaaat aaattgataa tttccatgtt cggattgtaa gcaagtaatg caccatcaat
     2341 actcaaagtg taaatcggca atttgtctag attgcctaca tggtctgcaa tggaatgttc
     2401 caatttattg taaaaaacta ttttttgaaa actgatagta aataataata attgacaaat
     2461 aacaatcccg gaaaatagta agttgattaa tttcggtttc tgctttaagc tcctgattat
     2521 attagtaaaa ggtagataat aaacaagtaa aactgcaggc acaacaaaca taagatacct
     2581 gttattctgg tagggattac atccaataaa aagactgtaa agcataatgg taaacatgat
     2641 tggaaaagtg taacgatgtc ggttttgctg cttaaataac aatatcaaaa acaatattcc
     2701 acaaaaaata aaggcagggt gaataaattg aaaaaaacca aatacaatat ttggggtagg
     2761 ataatgttgc cagccatcaa tattaaaaaa gctgcttttg aacaaattgg tgatactaaa
     2821 ctggtcggca aaaaaattgt aagagaatgc cggaccttca ttaccaatgt tccaaaatat
     2881 aaaccgctga cgtataatta aatccgggat ggtactcaat aacgctacaa tagccgcagg
     2941 taaaatgaaa aggtattgtt ttgtcgtgaa tatggttttc aaacaaataa aagccggcaa
     3001 taataataag attatacttg gatacctcgt cattagtgtt atacctgcaa aaaaagagga
     3061 taataacaag tatttcaact ttgcctgttg ttcaaatcgt aaactgtaat acaaagtaaa
     3121 aagccacatc gttatacaaa acatatcact catggcaatt aaactaaaac gtaaaatata
     3181 aggagacaat acaaagaaca ttaaaacaaa caagggtgca gagtgcgcat cggtagttat
     3241 taatttaatt attttcacca ataaatatgc agcaattaac atcgaaatca tgcttacaaa
     3301 ttgcatggca aacatggtac ttggaaaaaa gaagtctgca atactgcaaa ctattggata
     3361 catgatggga tatacggtgt aagcaggagc agtgccgtca ttataaaaag cagttaacga
     3421 attggttatg cgcaaatatt cataactgtc ctgaccaaaa aaaccattaa agtgataaaa
     3481 aacaatgagc accgtttgca aaatagctgc aaccgtgagc aggaaaaata atggacgttt
     3541 agaaatattg gatagcaatg gtttacctta tgcaggtaaa tataatcata atcttaaatc
     3601 atcgtcatct tcatacaact tagctgtatt ttcaaactgc tgcttaagtt cttctttttt
     3661 taactgacgt tgataaatta ggtctgccag cactgtcggt gcatcttccg gtaaatgttt
     3721 gcccaataaa gactttgctt tttcctcatc aacatctata cgatagagta tccaaagtag
     3781 tttctcgaaa ttatgatcta tcagaaatgc aatcatttca gcaagtgaat ttttaaatgc
     3841 atcagctgaa gttacttcca gcatttgttc ttccactgtc aaatcacaca tttcagcaat
     3901 ttctgaaagt gagttgtgca tccaattttc ggttttagtc ataatcatgg tattacctta
     3961 tcgccaataa ctttaaatat catgccattt ttccatcacg ccggcgccaa acaatgcaaa
     4021 gtcgtatttt accgggtcat ttttatcaaa taatcttaaa ttttctgtta attctagtgc
     4081 tgcttgccag tctgtttgtt tgcgttccag caatccgagc gattttgcta ctcgctcaac
     4141 atgcacatcc agcgggcata tgagctgatg tgtccctatg tttttccaac agccaaagtc
     4201 tacgcctgtg gtgtttggtc ttaccatcca tcttaaaaac atattgatgc gtttgcaggc
     4261 acttttcgtg gccggagtag caatatgttt gcgcgttcgt tgcggcgcat gctcatcatt
     4321 aaaaaaagaa ttgtggaatt gaatgagcat tttttctaca gttttttctt tttcaacgcc
     4381 tttaaataaa aaggccgctt ccagactatc atgggcattg tagaattgct gaaagaagtg
     4441 taaaaaatat aaggtgtcgg tataattaaa agtgcggtgt ttaaaatggg cgaaacgttt
     4501 tacatcctgt tcagtatgcc caacaataaa atcatagggc gcattatcca ttaatgcaat
     4561 caaagtattt gcattattaa tgatggttgt tctttgaccc caagataaca tggcagtcca
//
LOCUS       DAORKW010000269         4599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_854_length_4599_cov_5.623019, whole genome shotgun sequence.
ACCESSION   DAORKW010000269 DAORKW010000000
VERSION     DAORKW010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_854_length_4599_cov_5.623019"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..2075
                     /locus_tag="PLB46_10605"
     CDS             171..2075
                     /locus_tag="PLB46_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HRF77013.1"
                     /translation="MQNIHAVLKQYWGYDVFRPLQEDIIRSVLDGNDTLALLPTGGGK
                     SLCFQVPAMAMDGICVVVSPLIALMKDQVHNLNKRDIAAVALFTGMTKREIDIALDNC
                     VYGKIKFLYVSPERLLTEIFRERFKKMKVNLIAIDEAHCISQWGYDFRPPYLQIAELR
                     PFFPDIPIIALTASATKKVCDDIQLRLAFKKNNLFRASFARPNIGFIVRETDNKEDKL
                     LDILNTVKGSGVVYVRNRKKTQDTAAFLLRHKVNAGFYHAGLENDIRNNKQESWINGK
                     TRIIVCTNAFGMGIDKPDVRCVVHLDIPESLEAYYQEAGRAGRDGHKSYAGLLYDEHD
                     FLQLQENFEKQFPPIEFIRKVYHHLGNYCQIAIGAGVGQSFDFDLIVFCKQYQLKPVE
                     TRNALKILEQHGYIYVSEAFNKLSTVNIPIDRETLYAFQIANKQYEPVIKLILRTSPG
                     VFDETVSIHESDLAYHLSAQVHQVKEILEFLHKQGIIVYSPLKTKPQVDFVQERINAE
                     HLLFDFKMMEHLKTSAELRLKAITGYVKNKSECRVKNLLRYFDEDTQNCNSCDICVER
                     NKLELSEKEFTLVFDWLKKQLLSQPMMPEALLKLKLPVRKEKVVETLTFLTDNKQIIH
                     TAENILLWKE"
     gene            2072..3157
                     /locus_tag="PLB46_10610"
     CDS             2072..3157
                     /locus_tag="PLB46_10610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF77014.1"
                     /translation="MKKIVFTVTNDITYDQRMDRISTALVEAGYDVTIIGFLKKRSVK
                     TLDKPYHQVRFKLLFLKGKLFFLAYNFRIFWYLLFHKFDIYCGIDLDTLLPVYMHAKM
                     RKKPCVYDAHEYYTELPEIVSRPLIKKMWVRLEQYLLPKIRYNYTVGAAIAKTLSDKY
                     HQPFEVIRNVPILLPDMPTVARENFILYQGALNMGRGLEPLMQAMANINSHLYIAGEG
                     DLSVELRAYAATLPHREKIRFLGYVRPDELKSYTQKAKLGINLVEHLGLSYYYSLSNK
                     FFDYIHAGLPQITMNFPEYKNLNDKYKVAVLIDKPEPQLIAEAVNKLLNDEALYQELS
                     NNALLAKNELNWQLESKKLLAIYQQIE"
     gene            3150..4274
                     /locus_tag="PLB46_10615"
     CDS             3150..4274
                     /locus_tag="PLB46_10615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF77015.1"
                     /translation="MSNPHLHIVAFNIPYPANYGGVIDVYYKIAALHAEGVQVHLHCF
                     EYGRKRADELNAICVSVNYYPRSAMWKGFLSDKPFIVQSRDAAALLKNLKKDDYPILF
                     EGLHCCYFLAHSDLMHRRKWVRMHNNEPAYYLHLGQNEKRFFNKIYFFAEYRRLMKFE
                     WVLSCAEKIWCISPQETNYYQHKYPQTSYLAAFNGHKQVSGLPGRGSYILYHGNLEVN
                     ENKEAAMFLVNEIFSKCTLPLIIAGNKPDQNLIAAAAKFKHITIVANPTEAALSELMQ
                     NAHIHYLPTFQTTGIKLKLLNALYNGRFCVVNQNMVQDTGLETCCHLANSPQQAIDVL
                     TALMQEEFTSSEISKRQTVLQQFSDTTEVKKITRLLKSLH"
     gene            complement(4246..>4599)
                     /locus_tag="PLB46_10620"
     CDS             complement(4246..>4599)
                     /locus_tag="PLB46_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF77016.1"
                     /translation="DKSLIKLKSDEALKRVGLQSKGFKMPHELSGGEQQRVVIARAIL
                     NDPVAIIADEPTGNLDPETSDDIMQLLIFLNKEYNTTILMATHNYNLLERFPARIIKT
                     ANGKLIANVETSING"
BASE COUNT         1533 a          736 c          878 g         1452 t
ORIGIN      
        1 attagaacgt ttattcatca atcagcattt acaattagga agtattcggg taagcacaag
       61 ttcagcactt tgggattata tacaaataaa cgaaaaatcg ctgttggaag aacttaccga
      121 ctttaaaaat aaattcgcat tctcaggtaa aaaataattt ttattagcct atgcaaaaca
      181 ttcatgccgt actaaaacaa tattgggggt atgatgtttt cagaccatta caggaagata
      241 ttattcgttc tgtattagat ggtaacgaca ctttagcttt gttgccaaca ggtggtggaa
      301 aatctttgtg ttttcaggtg cctgcaatgg caatggatgg tatttgtgtg gttgtatcac
      361 cgttaattgc tttaatgaag gaccaggtgc acaacctgaa taaacgtgat attgctgcgg
      421 ttgctttgtt tacaggcatg acaaagcgtg aaattgatat tgctctcgat aattgcgttt
      481 atggcaaaat aaaattttta tacgtttcgc ctgagcgatt gttgacagaa atttttagag
      541 agcggtttaa aaaaatgaaa gtcaacctga ttgctattga tgaagcacat tgtatttcac
      601 aatgggggta tgatttccgt ccaccatatt tacaaattgc tgaattgcga ccgttttttc
      661 cggatatacc aattattgcg ctcacggcat ctgcaacaaa aaaagtgtgt gatgatattc
      721 aattgcgctt ggctttcaaa aaaaataatt tattccgcgc aagttttgcc aggccgaata
      781 ttgggtttat tgtaagggaa acagataata aagaagataa actactcgat attttaaata
      841 cggtaaaagg ttcaggtgta gtgtatgtgc gcaatagaaa aaaaacgcag gatacagcag
      901 cttttttatt acggcataaa gtgaatgccg gattttatca cgccggttta gaaaatgata
      961 tccgcaataa taagcaggag agttggataa atggcaaaac aagaattatc gtctgcacca
     1021 acgcttttgg tatgggcatc gacaaaccgg atgttcgatg tgtggtgcat ctcgatattc
     1081 ctgaaagttt agaagcttat tatcaagaag ctggaagggc gggtagagat ggacataaat
     1141 cgtatgcggg attattatat gatgaacatg attttttgca actacaggaa aattttgaaa
     1201 aacaatttcc tccaattgaa tttattcgca aagtttatca ccatttgggt aattattgcc
     1261 aaatagccat tggagcagga gtagggcaga gttttgattt cgatttaata gttttttgta
     1321 aacaatatca attaaagccg gttgaaactc gcaacgcatt aaaaattctg gagcaacacg
     1381 gatatattta tgtgagtgaa gcatttaata aattgtccac ggttaatatt ccaatcgacc
     1441 gggaaacctt atatgcattt caaattgcca ataaacaata tgaaccggtg attaaactga
     1501 ttttgagaac ttctccgggt gtgtttgatg aaacggtttc gatacacgaa tcagatttag
     1561 cctatcattt atcagcacaa gtgcatcagg ttaaagaaat tcttgagttt ttgcacaaac
     1621 aaggtataat tgtatattct ccattaaaga ccaaacctca ggttgatttt gtgcaggaac
     1681 gtattaatgc agaacattta ctgtttgatt ttaaaatgat ggagcacctt aaaacaagtg
     1741 ctgaactcag attaaaggct attacgggat atgtaaaaaa caaatcagaa tgcagagtta
     1801 aaaacctttt gcggtatttc gatgaagata cacaaaattg caatagctgt gatatttgtg
     1861 tggaaagaaa caaactggaa ctttctgaaa aagagtttac tttggtgttt gattggctta
     1921 aaaaacaatt actcagccag ccaatgatgc cggaagcttt gctaaaatta aaactgccgg
     1981 tcagaaagga aaaggtagtt gaaacattaa cttttttaac ggacaataaa caaataatac
     2041 atacagctga aaatattttg ctctggaaag aatgaaaaaa atagtattta cagtaaccaa
     2101 cgacattact tacgaccagc gtatggaccg tatctccact gctttggtgg aagccggtta
     2161 tgatgtaact attattggtt ttttaaaaaa gcgttctgta aaaactttag ataaacctta
     2221 tcatcaggtt cgatttaaac tgttatttct caaagggaaa ttgttttttt tagcttataa
     2281 ctttcgtata ttttggtatt tgttgtttca caaattcgac atctattgtg gcatagacct
     2341 ggacaccttg ttacctgttt atatgcatgc aaaaatgcgc aaaaaacctt gtgtttacga
     2401 tgcgcatgaa tattacaccg aattgcctga aattgttagt cgcccattaa ttaaaaaaat
     2461 gtgggtccgc ttagaacaat atttgttacc taaaattcgc tataactata cagtaggcgc
     2521 agcaattgct aaaacattat ctgataaata tcaccaacca tttgaagtaa tcagaaatgt
     2581 accaatatta ttgcctgaca tgccaactgt agcacgcgag aattttattt tatatcaggg
     2641 tgcattgaat atgggtaggg gattggagcc actgatgcaa gctatggcca atattaattc
     2701 ccatttgtat atagctggtg aaggagattt gtctgttgaa ttaagagctt acgcagcaac
     2761 attaccacat cgcgaaaaaa ttcgtttttt aggatatgtt cgtcccgatg aattaaagtc
     2821 gtatacccaa aaagctaaat tgggtattaa cctggtggaa catctcgggc ttagttatta
     2881 ctattcactt tccaataaat ttttcgacta tattcatgcc gggctaccac aaattaccat
     2941 gaattttcct gagtataaaa atttgaatga caagtataag gtagcggtat taattgacaa
     3001 gcctgaacca caattaattg ctgaggcagt caacaaatta ttaaatgatg aagctttata
     3061 tcaggaatta agtaacaatg cattattggc aaaaaatgaa ctaaattggc aactcgaatc
     3121 aaaaaaattg ctagctatct atcaacaaat tgagtaatcc gcatttacat atcgttgcat
     3181 ttaatattcc ttacccggca aattatgggg gtgtaattga tgtgtattat aaaattgctg
     3241 ctttacatgc cgaaggcgtt caggttcatt tacattgttt tgaatatggc agaaaaagag
     3301 cagatgaact caatgcaatt tgcgtctctg taaattatta tccccgcagc gccatgtgga
     3361 aagggttttt atctgataag ccattcattg tccaatcgcg tgatgcagca gcgttgctga
     3421 agaacctgaa aaaagatgat tatccgattt tatttgaagg tttacattgt tgctattttt
     3481 tagcacacag tgatttaatg catagaagaa aatgggtgcg tatgcacaat aatgaacctg
     3541 cttattattt acatttaggt caaaatgaaa aacgcttttt taataagata tacttttttg
     3601 cagaatatcg tcgtctcatg aaatttgagt gggtgttaag ttgtgctgaa aaaatttggt
     3661 gcatctctcc acaagaaaca aattactatc agcataaata tcctcaaaca agttatctgg
     3721 cagcttttaa tggccataaa caggtaagtg gattacccgg tcgtggcagt tatattttat
     3781 accacggtaa tttagaagta aatgaaaaca aagaagctgc tatgtttttg gtaaatgaaa
     3841 tatttagtaa atgtacattg ccattgatca tcgcagggaa taaacccgac caaaatttaa
     3901 ttgctgctgc agcaaaattt aaacatatta ctattgttgc taatccgaca gaagcagctt
     3961 tgtcggagtt aatgcaaaat gcacatattc attatttacc tacttttcaa acaaccggca
     4021 taaaattaaa actgttaaat gctttatata atggaagatt ttgtgtggta aatcaaaaca
     4081 tggtgcagga tacaggtttg gaaacatgtt gccatttagc taacagccct caacaggcta
     4141 ttgacgtatt aacagcttta atgcaggagg aatttacttc atcagaaata agcaaacggc
     4201 aaactgtttt acagcaattt agtgatacga ctgaggtgaa aaaaattacc cgtttattga
     4261 agtctctaca ttagcaatta atttgccatt ggctgtttta ataattcgcg caggaaaacg
     4321 ttccaacaaa ttgtaattat gtgtggccat taaaatggta gtattgtatt ccttatttaa
     4381 aaaaataagt aattgcataa tatcatcaga tgtttcaggg tcgagattac ctgttggttc
     4441 atctgcaata atagcaaccg gatcattaag tatagctctg gcaattacaa cacgttgctg
     4501 ctcaccaccg gaaagttcgt gtggcatttt aaatcctttt gattgtaatc caactctttt
     4561 taaggcttca tcacttttta atttaatcag tgatttatc
//
LOCUS       DAORKW010000270         4588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_857_length_4588_cov_4.295169, whole genome shotgun sequence.
ACCESSION   DAORKW010000270 DAORKW010000000
VERSION     DAORKW010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_857_length_4588_cov_4.295169"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1429
                     /locus_tag="PLB46_10625"
     CDS             200..1429
                     /locus_tag="PLB46_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRF77017.1"
                     /translation="MSEQTPKSIISELAAAAGLQTLEKYAEVDSKKQRLFIGIPREIA
                     FQENRVPLTPDSVETLVAQGHRIVIETKAGEGARFSDKDYSEAGAQIYYDRKQVFEAD
                     IILKVTPPTEDEMSLFKHGQILISPLHLPTLKKEIINELSKKQVTALAFEYIKDESNT
                     YAFVRCMSEIAGSSAILIASELLADAVHGRGYLLGGLSGIPPTQVLILGAGVVGEFAT
                     RAALGLGATVKIYDNNVYKLSRLQNNIGERFYTSVINNRRLLNDIRTADVVVGAIHSE
                     NGRSPVIVTEAMVQEMKPGSVIIDVSIDQGGCIETSEVTTHDQPTFIKHDVIHYCVPN
                     IASRVPRTASYAISNITASTLMQFNEYGGLENLIRVLPGVRHGVYIYKGHLTNQYLGQ
                     KMGLKFTDINLLITSSM"
     gene            1622..2761
                     /gene="dinB"
                     /locus_tag="PLB46_10630"
     CDS             1622..2761
                     /gene="dinB"
                     /locus_tag="PLB46_10630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRF77018.1"
                     /translation="MGVDRVVVHMDLDTFFVSVERLKDSRLMHKPVLVGGTGDRAVVA
                     SCSYEARAFGVHSAMPMRQAKMLCPEAIVVRGDYEHYSKYSNMVTEIIHEKVPLYEKS
                     SIDEFYIDMTGMDKFFGTYTYVQELRNRVIKETGLPLSFGLSENKTVSKVATGEAKPN
                     NHLKIEYGIEKSFLAPLSVRKIPMVGEVTAQLLRKMGIVKIKTVQEMPLEIMDNILGE
                     NGKSIWKKANGIDNSPVTPYTERKSISSEETFESDTIDVKRMKTILAGMTERLAYQLR
                     KENKLTSNVAVKIRYSDFETHTKQLRIPYTSADHTLIKLVKDLFDKLYDRRVLIRLIG
                     VRFSGLVSGGYQINLLEDSEHIIKLYQEMDLLRKRFGEGAVKRVG"
     gene            3124..>4588
                     /locus_tag="PLB46_10635"
     CDS             3124..>4588
                     /locus_tag="PLB46_10635"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRF77019.1"
                     /translation="MLINELTNLLIKMYLNTHSAYSLRFGIMTPEKLLEEAKAKGVHS
                     FVLSDINNTSGWFELLADAKKIGINVKCGIEFKNGNQTLFIGIAKNQDGFAELNRYLS
                     GYLKQKLDIPPIAPPLINAYVIYPYHHGAVYQLRDNEFVGVRKKDLTRFRYEKHHLPK
                     TKTVALNTVTFAVKKDFNAHRILRAIDHNTIITKLIPEDIDDPLHQFETYDQLIADFG
                     EHRYLLDNAQQLIDNCSFEFEYHTNKNIKTFTGKEKEDIDMLLQLTYEGVTKRYGKLN
                     KTVQDRINKELEIVIQKGFVSYFLINWDIIRYAKSKNYFTIGRGSGANSLIAYCIGVT
                     DVDPIELDLYFERFINLFRATPPDFDLDFSWKDREDVTDYIFNKHGEEHTALLATYTT
                     FKSRSFIREVAKTFGLPKYEIDAMVKNYGESIFGDAVKIPDKIARSIYSYGGYLHGNP
                     NYLSIHAGGILLSDKPIYYYTATHLPPKGFATTQFDMH"
BASE COUNT         1424 a          736 c          996 g         1432 t
ORIGIN      
        1 gggctatgtg gagttgtgtt atcatatttc ctgtggcttt tattttcggg ccaatcagac
       61 atataccttt tgaacatcag cttatagatt gcagctttgg tgttattggg ttattggtgc
      121 tttatttcac cagaaaaaaa attaaactgc tggaacactt atcaattggt ggaaaataaa
      181 tttattgtat ctttaaaaca tgagtgagca gacccctaaa tctatcattt cggaactggc
      241 agcagctgcc gggttgcaaa cgttggaaaa atatgctgaa gtggacagta agaagcaacg
      301 attatttatt ggtattccac gtgaaattgc atttcaggaa aacagggttc cattaacccc
      361 ggatagtgtt gaaactttgg ttgcacaagg tcatcgaatt gtaattgaaa ctaaagccgg
      421 tgaaggtgcc cgttttagcg ataaagatta tagtgaagcc ggagcacaaa tttattatga
      481 tagaaaacag gtttttgaag ctgatatcat tttaaaagtg accccaccta ccgaagatga
      541 aatgagtttg tttaaacatg gacaaatatt aatttctcca ttacatcttc ctacattaaa
      601 aaaagaaatc attaatgagt tatccaaaaa acaggtaact gcacttgcat ttgaatatat
      661 caaagatgaa agtaatactt atgcctttgt tcgttgcatg agtgaaattg ccggtagcag
      721 cgcaatatta attgcaagtg aattattggc agatgccgtt catggccgtg gttatttatt
      781 aggaggttta tccggaattc cgccaacgca agtacttatt ttaggtgcag gtgttgttgg
      841 agaatttgct acacgtgctg ctttgggttt gggtgctacg gtaaaaattt atgataataa
      901 tgtttataaa ctttctcgtt tacaaaataa tattggcgaa cgtttttata cttcagtaat
      961 taataatcgt cgtttattaa atgatatcag aacagccgat gtggtagttg gagctattca
     1021 ttccgaaaat ggtcgttcgc cggttattgt cactgaggcg atggtgcagg aaatgaaacc
     1081 cggcagtgta attattgatg tgagtatcga ccagggtgga tgtatcgaga ccagtgaagt
     1141 cactacacat gatcagccta cttttattaa acatgatgta atacattatt gtgtacccaa
     1201 tatcgcttcg cgagtgcctc gtactgcaag ttatgctatc agcaatatta cagccagtac
     1261 attaatgcag tttaatgaat atggcggctt agaaaatctt atccgtgtat tgccgggagt
     1321 tcgtcatggg gtttatatct ataaagggca tttgactaat cagtatctcg gtcagaaaat
     1381 gggtttaaaa tttaccgaca ttaatttact tattacgagt tcgatgtaat agatttcccg
     1441 gtattggaat ctggtatgag atttggatta ttcccctagg tttttctgca tttccatgca
     1501 tgtcagatat actgactatt catttccttg cttttattga tgtttataac tttttttgac
     1561 aattttattg aaaatgtcaa caatatgtca ttatatttga ctttcaaagt tggaggtcat
     1621 tatgggagtt gatcgtgtgg tggtgcatat ggatttagac accttttttg tttcggtgga
     1681 gcgattaaaa gattcgcgtt taatgcataa gccggtattg gttggaggaa cgggtgatcg
     1741 tgcggtggtg gcgtcgtgca gttatgaagc gcgggcgttt ggggtgcatt cggcaatgcc
     1801 gatgcggcag gcaaagatgt tatgtccgga ggcgattgtt gttcgtggcg attatgagca
     1861 ttattcgaaa tattcgaata tggttacgga gattattcat gagaaggtgc ctttgtatga
     1921 aaaatcgtcg attgatgagt tttatattga tatgacgggc atggataagt tttttgggac
     1981 gtatacttat gtgcaggaat tgcggaacag ggtgataaaa gaaactgggc ttccgctctc
     2041 ctttgggtta tcggaaaaca aaacagtttc gaaggtggca acgggagaag caaagccgaa
     2101 taatcacctc aaaattgaat atggtattga gaaatcattt ctggcgcctt tatcggtccg
     2161 caaaattccg atggttggag aagttactgc tcagttgttg cgcaaaatgg gcattgtaaa
     2221 aattaaaaca gtgcaggaaa tgcccctgga gattatggat aatattcttg gggaaaatgg
     2281 gaaatcaatc tggaaaaagg caaatggtat tgataattcg ccggtaacac cttatacgga
     2341 acgcaaatca atttcgagtg aagaaacatt tgaaagtgat actatagatg tgaaacggat
     2401 gaaaacaatt ttggccggga tgactgaacg gcttgcatat cagttgcgta aggaaaataa
     2461 actgacttcc aatgttgcgg taaaaattcg gtactccgat ttcgaaacac ataccaaaca
     2521 attacgtatt ccctacactt ctgctgatca tacattaata aaactcgtaa aagatttatt
     2581 cgataaacta tacgaccgtc gtgtattaat ccgattaatt ggtgttcgtt tttcgggatt
     2641 ggtaagcgga ggatatcaaa ttaatttatt agaagattct gaacatatta ttaaactcta
     2701 tcaggaaatg gatttgttga gaaaaaggtt tggggagggg gcggtgaaga gggttgggtg
     2761 atgtgctgat ttgttaatgt gctgatgtgc tgattaacag ccggatgatg taccaatttg
     2821 tgaatgtacc aattaacagc cggatgttga gttggaatgt aattcggagc taccgtagtc
     2881 gtcaacccac gtgttgacct ggggtttgta ataattacat tcggagatgt gttgatgtac
     2941 caatgtgtga atgtaccgat gtaccaatta acagccggat gctgcgttgg aatgtaattc
     3001 ggaggtttgc atggatacct ggacaaaacc aaagtggtgc aaccacttta aaaacgaaaa
     3061 ttgaagttca ttagccgatt aatttgttgc ttatcgaaca ccatcatttg ctaattagct
     3121 aatttgctaa ttaacgaatt aaccaatttg ctaattaaaa tgtatttaaa cacccactct
     3181 gcatatagcc ttcgtttcgg gataatgacg ccggaaaaat tgcttgaaga agcaaaggct
     3241 aaaggtgtgc atagttttgt gctgagtgat atcaataata cttcgggatg gtttgaattg
     3301 ttagccgacg ccaaaaaaat tggcattaat gtgaaatgtg gtatcgaatt taaaaatggc
     3361 aaccaaacat tatttatcgg tatcgcaaaa aatcaggatg gttttgcgga gttaaacaga
     3421 tatttgtcgg gatatctgaa acaaaaactg gacatccctc ctattgcacc acctttaatt
     3481 aatgcgtatg tgatttatcc ttatcatcat ggcgctgttt atcaactgcg tgacaatgaa
     3541 tttgtgggag tcaggaaaaa agatttaacg cgattccgct acgaaaaaca tcatctcccc
     3601 aaaacaaaaa cagtagcatt aaatacggtg acttttgctg tgaaaaaaga ttttaatgcg
     3661 caccgtattt tacgagcaat tgaccacaat accatcatca ccaaactgat acctgaagat
     3721 attgacgacc ccttacatca attcgaaacg tatgatcaat taattgccga ttttggcgaa
     3781 catcgttatt tactcgacaa tgctcaacag ctgattgaca actgttcatt tgagtttgaa
     3841 tatcatacca acaaaaacat caaaaccttt acaggtaaag aaaaagaaga tattgacatg
     3901 ttgttgcaac tcacttacga aggtgtaaca aaacgatatg gcaaattaaa taaaactgtg
     3961 caggaccgta ttaataaaga attagaaatt gttattcaaa aaggattcgt ctcttatttt
     4021 ttaattaact gggatattat tcgatatgcc aaaagtaaaa attatttcac cattggtcgc
     4081 ggcagtggtg cgaatagttt aattgcctat tgtattggtg ttactgatgt agacccaatt
     4141 gaactggatt tatattttga acgatttatt aatttattca gagccactcc ccctgatttt
     4201 gatttggatt tttcgtggaa agacagagaa gatgtaactg attatatttt taataaacat
     4261 ggcgaagaac atactgcgct gctggctacg tataccacgt ttaaaagtcg ttcgtttatt
     4321 cgtgaagtag ccaaaacatt tggattacca aaatatgaaa tcgatgccat ggtgaaaaat
     4381 tatggtgaaa gtatatttgg tgatgcagtg aaaattcccg ataaaattgc aagaagtatt
     4441 tattcctacg gtggttattt acatggtaat ccgaattatc tgagtattca cgccggaggt
     4501 attttgctga gcgacaaacc aatttattat tataccgcaa cacatcttcc ccccaaagga
     4561 tttgctacta cacaattcga catgcatg
//
LOCUS       DAORKW010000271         4575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_861_length_4575_cov_5.553319, whole genome shotgun sequence.
ACCESSION   DAORKW010000271 DAORKW010000000
VERSION     DAORKW010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_861_length_4575_cov_5.553319"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1422
                     /locus_tag="PLB46_10640"
     CDS             205..1422
                     /locus_tag="PLB46_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRF77020.1"
                     /translation="MLLQQVNMKDFAYDLPESKIAQHPLPERDASKLLIYRDGKIKDD
                     IYRNVYQYIPEQSMLIFNNSKVIPARLMFSTASGASIEIFCLEPAKENAEITTEMQQQ
                     GSVQWKCLVGRLAKWKEPTLSIITDSFTLSATKKEQSGNAYIIAFSWTPVSLSFANIL
                     EHFGAMPIPPYLKREATTNDSTRYQTIYAAQQGAVAAPTAGLHFTEYVFSALKSKQTQ
                     IEYITLHVGAGTFKPVKGDTIADHEMHAEWIEVEIALIQKIIQQINNSHAVIAVGTTS
                     LRTIESLYWMGVKAFVNPDISSHDLPIKQWEVYALPQDVDPIGALQALIQWLQKNNHD
                     KLICKTQILIVDTYRLKLITALITNFHQPSSTLLLLIAAVVGNEWKPIYQHALDNNYR
                     FLSYGDGSLLFTA"
     gene            1818..2096
                     /locus_tag="PLB46_10645"
     CDS             1818..2096
                     /locus_tag="PLB46_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77021.1"
                     /translation="MKYIVNSIELDNPSLPTSSQLLDKTPDIYNFHNVSARGAINHKH
                     KQNNKISQQAAIPVHYQQNYTMTGYKIDNSNNIAATYFHYKTQPFTLM"
     gene            2540..2968
                     /locus_tag="PLB46_10650"
     CDS             2540..2968
                     /locus_tag="PLB46_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF77022.1"
                     /translation="MKKIILAEDDINDCELFEDALLELDIKTQLTISRDGVELMSTLD
                     ETVPPRPDAIFIDLNMPRKNGYECLTEIRNNEKLKDIMVVIFSTTNNKSAIEKTFSLG
                     ANYFVCKPCSFELLKKALEKVLHFTHLGPAEHQQQIIEVR"
     gene            2986..>4575
                     /locus_tag="PLB46_10655"
     CDS             2986..>4575
                     /locus_tag="PLB46_10655"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRF77023.1"
                     /translation="MIKQVKPSLPQVRRFYLLTTLFLVGLSFYTFFQIKSLISSSEWI
                     NHTNKVNSSLDKVSNAFVNAVSNQKTYLVTGDTTKIIARNADFFTIDTQLNVLDSLTK
                     DNPDQAANLLVLRKTIHEKRANLDVLLQTYPPFEISNSLANSLADALHKTELVNYEID
                     KMSTFENNLLVERNKNYTQLSTITPLFIITLSLGAVLILLGFYIRLNNVIHELQDVHV
                     KLDISNKELENKNKELIKTGEQFLKMFDNNPVALTFGEIDSDKIVYANNAFFSAFGYS
                     KAEVINHTADELNLVSQEETARLLPIILGHLQENRTVEELQALPGEERAELLLQLREK
                     MFQNGFEVSYTRKTGETFIAIVFYEIIDIGNIKYALKSYVDITEHKEALKEIEDQKAF
                     SELIIASDPAMVFATDENMHITVWNKKVEVHSGLRKEETVGKHILDIYPEYNNEQWQR
                     LLHSVLKDGKSLHFSKVEFKRRKGFGESWIIPLRNSSQNIIGILAITRDITETTEMNV
                     RLEKINADLAKINEELNNTTAT"
BASE COUNT         1640 a          799 c          750 g         1386 t
ORIGIN      
        1 atcaaattac caataaacct caatataact atgggcttca gccctgaagt gaaaatttta
       61 agccccccgg cgaaaaattt tctacttcag ggcagttttt gatttttact ttacatcaat
      121 caattgatta actttgtgtc gataaatagg aaatatactt aactatttta cactaaaaaa
      181 ccagcggcta ataatattgc tgatatgttg cttcaacaag taaatatgaa agatttcgct
      241 tacgatttgc ctgaaagcaa aatcgcacaa catccattgc cggagcgaga tgccagtaag
      301 ttgttgattt accgtgatgg gaaaataaaa gatgacattt atcgaaatgt atatcaatat
      361 attcccgaac aatcaatgct gatatttaat aattcaaaag taattccggc gcgattaatg
      421 ttttcaactg catcaggtgc aagcattgaa atattttgtc tcgaacctgc aaaagaaaat
      481 gccgaaatca ccaccgaaat gcaacaacag ggttcagtgc aatggaaatg tttggtggga
      541 cggttagcaa aatggaaaga gcccacattg agtataataa ctgattcgtt tacattatct
      601 gcgacaaaaa aagaacaaag tggtaatgct tacatcatag cattttcgtg gactcctgtt
      661 tcattatcgt tcgcaaacat tctggaacat tttggggcta tgccaattcc accttatctg
      721 aaacgtgagg caacaacaaa cgacagcacc cgttatcaaa ctatttatgc agcacaacaa
      781 ggcgcagttg cagcacctac tgcaggatta cattttactg aatatgtatt tagtgcatta
      841 aaatcgaaac aaacacaaat cgaatatatc acattacatg ttggtgccgg cacatttaaa
      901 ccggtaaaag gtgataccat tgccgaccac gaaatgcatg cagaatggat tgaagtagaa
      961 atagctttaa tccaaaaaat aattcaacaa ataaataata gtcatgctgt tattgctgtt
     1021 ggcaccactt cattaagaac aattgaatca ttgtattgga tgggtgttaa ggcatttgta
     1081 aatcccgata tttctagtca cgatctccct atcaaacaat gggaagttta tgcactacca
     1141 caagatgtgg accctattgg tgcactacag gctttaatac aatggttaca aaaaaacaat
     1201 cacgataagc ttatttgtaa aacacaaata ctgattgttg atacttatcg tctaaaattg
     1261 ataactgcgt taattactaa ctttcaccaa ccatcttcta ccctgttgct attaattgca
     1321 gctgtggttg gtaatgaatg gaaacctatt tatcagcatg ccttagacaa taattatcgg
     1381 tttttaagtt atggagatgg aagtttgtta tttacagcat aatagttgtt attcaaccaa
     1441 tcgccatgtc tctggttatt gtaaatagac caagaaaatc aaacgcttat acatatataa
     1501 actgactacc gtttccattc acatgtcgac tttaatttct tgatgtcaca aacgatacta
     1561 ttgctaagtt atatgcctat ttttctaccg gattgattat catatagata tgccattctg
     1621 acgcatttct aattgaaagt agtaactcaa acatactata ttatttggta taacaattaa
     1681 catccatgct tgtgacaaat tttacataat tttcaaaatt atttacaaga ttagtagaat
     1741 tttcaaccca ttagttggca gttcctccgc gattttatag ttttccgaaa aaattattat
     1801 accaattaaa tactagaatg aagtatatag taaatagtat tgaacttgac aacccatcat
     1861 taccaactag cagtcagctg ttagataaaa cccctgatat ttataatttc cataacgtat
     1921 ctgctcgtgg tgcaataaat cacaagcata aacaaaacaa caagataagt cagcaagcgg
     1981 ctattcccgt tcactatcag caaaattaca ccatgacagg atataaaata gataacagta
     2041 ataatatagc cgcaacttat tttcattaca aaacacaacc ttttacttta atgtagtttt
     2101 aaacagttgt aaatcattca ataacagcaa cttctaataa aatttgcaaa gacgcttgtt
     2161 tttctcaaaa taatttttta tctttaaata ccactttata caaataatgc cgaagtggta
     2221 cccgctaaat gtaaaattta cggaagcatc agaatgtgta atcagctaaa tcaggtttac
     2281 ctgattaatt atcccataaa taatacacca cacactattc ttttagctta atcaagcgtt
     2341 gaaagtaggt atttgtaaac ccaaattcct tcatttaaaa tcaatcacaa gccaaccagt
     2401 ataggcgata acaattaaat taaacacaat ggtgttgata tttagttatt gctcctatta
     2461 attaacattt tacatagttt aaaattatta ctttcttaag aagtagtaaa taatactgta
     2521 tgcaaataaa ttaaaaaata tgaaaaaaat tatcttagct gaagatgata tcaatgactg
     2581 tgaattattc gaagatgctt tgctggaatt ggatataaaa acacaattga ctatttccag
     2641 agatggtgta gaattaatgt ccaccttgga cgaaaccgtc ccgccccgtc cggatgccat
     2701 ttttatcgat ttaaatatgc ctcgaaaaaa cggctacgaa tgtttgactg aaattcggaa
     2761 taatgaaaag ttgaaggata ttatggtggt tattttttca accacaaaca acaaatcggc
     2821 aattgaaaaa accttttcat tgggtgccaa ctattttgtt tgtaaaccat gctcatttga
     2881 attattaaaa aaagcacttg aaaaagtatt gcattttacc catctgggac ctgctgaaca
     2941 ccaacaacaa ataattgaag tgcgataaat tttaaggtaa ttaatatgat aaaacaagta
     3001 aaaccatcgc tgcctcaggt taggagattc tatttgctta ctacgttgtt tttagtgggc
     3061 ctctcctttt atactttttt tcagataaaa agtttgatca gtagttcaga atggataaat
     3121 catactaata aggtcaacag ttctctggat aaggtgtcga atgcttttgt taacgctgtg
     3181 agcaatcaga aaacatatct ggtaactggt gatacaacta agataattgc cagaaatgca
     3241 gatttcttta ctatagatac acagttaaat gttttggata gtttaacaaa agacaatccg
     3301 gaccaagctg caaatcttct tgtattgcgc aagacgattc atgaaaaaag agcaaatctc
     3361 gatgtcttac ttcaaactta tcccccattc gaaatttcaa acagcttggc caatagttta
     3421 gcagatgcac tgcataaaac cgagctagtt aactacgaaa ttgataaaat gtcaactttc
     3481 gaaaacaatt tattggtaga acgcaacaaa aattacacgc aactttccac tataacacca
     3541 ctatttatca ttaccctttc gcttggggct gtgttaatcc tcttaggatt ttatatccga
     3601 ttaaataatg taatacatga attacaagat gtacatgtaa aactggatat ttccaataag
     3661 gagctagaaa acaaaaacaa agaactaatt aaaactggcg aacagttttt aaagatgttt
     3721 gataacaacc ctgtggctct cacttttggt gaaatagatt cagataaaat tgtatatgct
     3781 aataatgcat tcttttccgc tttcggttat tccaaagctg aagtaatcaa tcacactgct
     3841 gacgagttaa atctggtatc ccaggaggaa acagcccgtt tgttacccat tattttaggc
     3901 cacctgcagg aaaatcgaac ggttgaggag ttacaagcac ttcccgggga agaaagggca
     3961 gaattattat tacaattgcg ggaaaaaatg tttcaaaacg gttttgaagt aagttataca
     4021 cgcaaaaccg gagagacatt tatcgcgata gttttttatg aaattattga tattggcaac
     4081 attaaatatg cacttaaatc ttatgtggat ataacggaac acaaagaggc acttaaagaa
     4141 attgaagatc aaaaagcttt ctctgaatta attattgcca gcgaccctgc aatggttttt
     4201 gctacagatg aaaatatgca tataacagta tggaataaaa aagtggaagt ccactcaggg
     4261 ttaagaaaag aagaaactgt aggtaaacat attttagaca tttatccgga atacaataat
     4321 gaacaatggc aacgcctcct gcattcagta ttgaaggacg ggaaatcact acatttttca
     4381 aaagtggaat ttaaacgtcg taaaggtttt ggagaaagct ggattatccc gttaagaaat
     4441 agtagtcaaa atattatagg cattttggca attacccgag acataacgga aaccactgaa
     4501 atgaatgtac ggcttgaaaa aattaatgcg gatctggcta aaataaatga ggagttaaat
     4561 aataccactg ctacc
//
LOCUS       DAORKW010000272         4569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_865_length_4569_cov_4.627160, whole genome shotgun sequence.
ACCESSION   DAORKW010000272 DAORKW010000000
VERSION     DAORKW010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_865_length_4569_cov_4.627160"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..3137
                     /locus_tag="PLB46_10660"
     CDS             174..3137
                     /locus_tag="PLB46_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77024.1"
                     /translation="MKKVLTLLTAVLCLTVSNAQQVHQKDPYLKTFIQNSKRLPDPQL
                     QAQLRDAQAWESFRETNGRWWVEFKEETGTPHRAFGAPIATSGVTAPERALNFIANNL
                     QAYEINTADLRLIQNHTSAKYHYVTFIQQYNGLDILWSEATVRLNMNNQVCMFGMDIY
                     NNIEINTTPAINAEGIKSYAAADFGIVVNSVDVVAIPKILPIPGPDGLEYKLVYETTV
                     NTSNANGIPGKYYSLVDANNGKVYYRSNRVHECGEYLMGADLQANSSITDNPLIPTVN
                     RGLPYLRLEIDGDFYYTDENGHIAFNFITTPTPATVELRGLYARVSQGLGDANIESID
                     ITIFPGENTIEFDDLSGAVNSEVSAYYWQNIVHDHLKSYFPDFTDLDFDQLIRVERND
                     GSCNAFYDGSSTNFYEAGGGCPATGLFNDVVMHEYGHGVNYDLYADLGDPGGMNNGAM
                     QEGYADLWGITVTDNPIMGQGFLGGAGTNVRRYDVEPKVYPQDLVGEVHADGEIIAGA
                     WWDVNENFGFDLVSMTDLWMETHNATVDGAAGNEGEIFRDVLLEALMADDDNGNLDDG
                     TPNDDAITEAFCEHGITLIGNISLDHEEFEAPVAELTPVAIETTLDVDYPEFIGGANI
                     YWRTTPGAAYTITEMTDLGGATYEASLPAQPIGTIIEYYFKVDDTNGCGGVTLPKKAD
                     QAVEPNLPYFVLVGFNLIEYEDFDNEFGSWEVDPFDSDEATTGVWDVNTPIASTDDFG
                     NTIQTGTDHTEGPGNLCAFTANAGGGDAIGTQDVDGGETTLRSPFFDLTAYEDPVFSY
                     YRHYSNASPTSANPGNDVWEVYITDNGTDWIKVERTHTEDNTWRRNVVRVLDYVDNTE
                     DVAFIFIAEDSLRADDASGFNGGSIVEAAVDDLFLYDVGEPVIQSVNESDIIAGVFPN
                     PANELVNVYFGNITGDVNLVLTNTIGETVLTDKFNVTNGSQYTFATTKLAQGVYTLTI
                     SNDGTVANKQIIIQH"
     gene            complement(3371..3736)
                     /locus_tag="PLB46_10665"
     CDS             complement(3371..3736)
                     /locus_tag="PLB46_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77025.1"
                     /translation="MSITLYPDPIINAGPDVDIPLGGYTVLNASGATTYLWNEDPNLS
                     AYDIANPTSSPQDTTTYIVYGTDANGCIGFDSVAVVVIVEPGIHSPNAFSPNGDGFND
                     VYKPILLACNLNEYSIYDR"
     gene            complement(3888..>4569)
                     /locus_tag="PLB46_10670"
     CDS             complement(3888..>4569)
                     /locus_tag="PLB46_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF77026.1"
                     /translation="LDGSGGVTYSWMPPVYLDDPTLEDPTVIDPATTMYYQMVAYSTL
                     GCSDTDNVVATLNPLPIIDAGQDQIMVLGGFTELNATGASTYTWTPIESLSDPNVSNP
                     TAFPEDTTMYVVTGTDLNGCVGTDTVIVYVVEDSDIATPNAFTPNGDGLNDVFKPSFV
                     GLGEITDFYVYNRWGKLLYFSVDPTKGWDGSMNGIEQEVGTYLVVIKGVNQFGDEVMK
                     TGTIALLR"
BASE COUNT         1466 a          812 c          859 g         1432 t
ORIGIN      
        1 ttttggcctt ttccccaaac gatttaaccg ttgtttataa gtcgcaacag ggctttttat
       61 gacttgtgga tgaccaacca aacaattatc tttaccttct gttatatcag gcaaacaacc
      121 actaactgtt agattataac atatttatta atcatcaaac cgtcacatca attatgaaaa
      181 aagtgcttac actcctcacg gcagttttat gcttaaccgt gtccaatgca cagcaggttc
      241 atcaaaaaga cccttactta aaaacgttta tccagaactc aaaacgtttg ccggatccac
      301 aattacaagc tcagttgcga gatgcgcaag cttgggaatc attccgcgaa actaatggaa
      361 gatggtgggt agaatttaaa gaagaaacag gtacgccaca ccgtgcattt ggagctccaa
      421 ttgctacatc cggcgtaacc gctccagagc gcgctttaaa ttttattgct aataatttgc
      481 aggcttatga aattaatact gctgatttac gattaattca aaatcatact tctgcgaaat
      541 atcattatgt aacttttatt cagcaatata atggtttaga tatcctttgg agcgaagcaa
      601 ctgtgcgatt aaatatgaac aatcaggttt gtatgtttgg tatggatatt tataataata
      661 ttgaaataaa tactacacca gcaatcaatg ctgaaggcat taaatcatat gccgctgccg
      721 attttggtat cgtagtaaat agtgttgatg ttgtagctat tcctaaaatt ttacctattc
      781 ccggtcctga tggattagaa tataaattag tttacgaaac aactgtaaat acgtctaatg
      841 caaatggtat tccaggtaaa tactacagct tagtggatgc taacaatggt aaagtttatt
      901 accgcagcaa tcgcgttcac gaatgcggtg aatatttaat gggggctgat ttacaagcca
      961 atagcagcat tactgataac cctttaattc caacagtaaa ccgtggatta ccctatttga
     1021 gattagaaat tgatggtgac ttttattata cagatgaaaa tggccacatt gcatttaatt
     1081 ttattacaac tcctactcct gcaacagttg aattacgcgg cttgtatgca cgtgtaagtc
     1141 agggattagg tgatgcaaat attgaaagta ttgatataac tattttccct ggtgaaaaca
     1201 ccattgaatt tgacgacttg tcaggggcag ttaattctga agtttctgca tattactggc
     1261 aaaatatcgt gcacgaccat ttaaaatcat atttccccga ttttacagat ttggattttg
     1321 atcaactaat cagagttgaa agaaatgatg gttcatgtaa tgcattttat gatggttcaa
     1381 gcaccaactt ttatgaagcc ggcggtggtt gtcctgcaac aggtttattt aacgatgtgg
     1441 taatgcacga atatggtcat ggtgttaact atgatttata tgctgattta ggtgacccgg
     1501 gtgggatgaa taacggtgct atgcaagaag gttatgctga cttgtgggga ataaccgtaa
     1561 ctgataatcc aattatggga caaggatttt taggtggagc aggtactaac gtaagaagat
     1621 atgatgttga acctaaagtg tacccgcaag atttagttgg tgaagtacat gccgatggtg
     1681 aaattattgc cggcgcttgg tgggatgtaa atgaaaattt tggttttgat ttagtttcca
     1741 tgactgactt atggatggaa acacataatg ccacagttga tggtgcagct ggtaacgaag
     1801 gcgaaatatt tcgtgatgta ttattagaag cattaatggc tgatgatgac aatggtaatt
     1861 tagatgacgg aacgccaaat gacgatgcca ttacagaagc attttgtgaa catggcatta
     1921 ctttaattgg taatattagt cttgatcacg aagagtttga agctccagtt gccgaattaa
     1981 ctccggttgc aatagaaaca actttagatg ttgactaccc tgaatttatt ggtggtgcca
     2041 atatttattg gagaaccact ccaggtgctg cttatactat taccgaaatg actgatttag
     2101 gtggtgcaac ttatgaagca agtcttcctg ctcaacctat cggaacaatt atcgaatatt
     2161 attttaaagt tgatgatact aatggatgtg gtggtgtaac attacctaaa aaagctgacc
     2221 aggctgtaga accgaattta ccatacttcg tattagttgg ttttaattta atcgaatatg
     2281 aagattttga taacgagttt ggcagttggg aagttgaccc atttgattca gatgaagcta
     2341 ctaccggtgt atgggatgta aatacaccaa ttgcatcaac tgatgatttt ggtaatacaa
     2401 ttcaaaccgg cactgaccat actgaaggtc cgggtaattt atgtgcattt actgcaaatg
     2461 ccggcggtgg tgatgctatc ggaacacagg atgttgatgg tggtgaaact acgttaagaa
     2521 gtccattctt tgatttaaca gcttacgaag accctgtttt tagttattac agacattatt
     2581 caaatgcatc acctactagt gcaaaccctg gtaatgatgt atgggaagtt tatataactg
     2641 ataatggtac cgactggatt aaagttgagc gtacacatac tgaagacaat acatggagaa
     2701 gaaatgttgt tcgcgtatta gattatgtag ataatactga agatgttgca tttattttta
     2761 ttgcagaaga tagcctccgt gccgatgatg catccggttt taatggtggc agcatagttg
     2821 aagcagctgt tgatgattta ttcttatatg atgttggcga accggttatt caatcagtaa
     2881 atgaatcaga tattattgca ggtgttttcc ctaacccggc aaatgaactc gtaaatgttt
     2941 attttggaaa tattaccggt gatgtaaatt tagtattaac taataccatt ggtgaaactg
     3001 ttttaactga taaatttaat gttactaatg gaagtcagta cacttttgct acaacaaaat
     3061 tggcgcaagg tgtttataca cttaccattt ctaatgatgg aacagtagcg aataaacaaa
     3121 taattattca gcattaattc aaacaattaa tgcaactaaa aaggcgaatg gaatttcttc
     3181 cattcgcctt tttttatgat aatgaaataa attatttcac caatgtaata ctagtttgta
     3241 attgtaaggg atcacccttc aatgaagttg cacttattat caccacataa gcacctaatt
     3301 caccctcttt gccattaata aaaccattcc atccattatc aatattggat gaaacaaaaa
     3361 cttgttcacc ctatctgtca taaatactat actcatttaa attacaagcc aataaaatag
     3421 gtttataaac atcattaaag ccatcgccat ttggagaaaa tgcatttggt gaatgaatac
     3481 ccggttcaac aattaccaca actgccaccg aatcaaatcc gatacatcca ttcgcatctg
     3541 ttccataaac tatatacgtt gttgtgtctt gtggagatga tgttggattt gcaatatcat
     3601 aagccgacaa atttggatct tcattccata aataggttgt tgcgccactt gcatttaaca
     3661 cagtatatcc acccaaagga atatcaacat caggtcctgc atttataatt gggtcgggat
     3721 ataaagtaat actcacatca tcagtgtctt tacatccatg aatatcggta cctacaacta
     3781 aataaattac atctgttaat ggatcaactc ataatgcaga taaaaaaaaa tgcataatta
     3841 aatggagccc attcaattat gcattttata agttaattaa ttatttttta tcgcaataat
     3901 gctatggttc ctgttttcat tacttcatca ccaaattggt taacgccttt tataacaaca
     3961 aggtatgttc caacttcctg ctcaatacca ttcatactac catcccaacc tttagtaggg
     4021 tcaactgaaa aataaagtaa ttttccccag cggttataaa cataaaaatc agttatttcc
     4081 cctaatccaa caaaagacgg tttaaataca tcatttaatc catcaccgtt aggtgtaaat
     4141 gcatttggag tagcaatgtc agaatcctct acaacataaa caattacggt atctgtgcca
     4201 acgcaaccat ttaaatcagt tccggttact acatacatcg ttgtatcttc cggaaatgcc
     4261 gtaggatttg aaacgttagg atctgaaagt gattcaatcg gcgtccaagt ataagtggaa
     4321 gcgcctgtag catttaattc agtaaatccg cccaatacca ttatttgatc ttgtccggca
     4381 tcaattattg gcaatgggtt taaagttgca acaacattat cggtatcaga acaacctaaa
     4441 gtgctatatg caaccatctg ataatacatg gtagtagcag gatcaattac tgtagggtcc
     4501 tcaagtgttg ggtcgtcaag ataaaccggt ggcatccaac tatacgtaac tccacctgag
     4561 ccatccaat
//
LOCUS       DAORKW010000273         4568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_867_length_4568_cov_5.006869, whole genome shotgun sequence.
ACCESSION   DAORKW010000273 DAORKW010000000
VERSION     DAORKW010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_4568_cov_5.006869"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..3120
                     /locus_tag="PLB46_10675"
     CDS             25..3120
                     /locus_tag="PLB46_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF77027.1"
                     /translation="MLKNKIQFVIFLLLICAFRQVQAQQSMIYTDLYKDYKKGMELFE
                     NQMYSTAQELFNTVGAIKPEGTDGEIITFVANSQYYGAICAIELQNPDAEKRMLDFVE
                     NNPGHVLLGSAYNQLGKIYYNKKEYTNAVTWFEKVDVKDLSLVERDLYKFQFGYSLFN
                     KKRFTEAKVMFAQLKDKANSPYYYGANYYYGFIQQEEGNYENALSCFEIVKDIPPYSS
                     VVPYYMTNIYFQQKKFEKVISYAEPLMADTKLVYYAEINSLLGQAYFYKENYKKALPY
                     LSYYIEKAKNIRDEDYYQLAFTHYQTKDYNAALATLKETSDDDDTLYQHIQFLYGNCY
                     IELNKKDEARNAFMEASRIGVDAEIHESALFNYGKLSYESAFYTAAVSALQDYLKKYP
                     KGKKKTEAQELLASSLLNTNDYVAAMEVIDNIPQKTPKLKEAYQKVSYYSGVQLFNQK
                     NYNEADKMFNQSLENPIDADLQAACYFWIGEIRYTENKYALAINNHVKFQDLSKVAKD
                     LPEETTIPYSNYTIGYSYFKQKSYSNASNYFSKVTTSLKTSKTDITQNNVYIDAVLRL
                     GDCYFMTKDYKKAEQNYSKIIDNNSKGVDYALYQKGMLQGLQNNNNGKISTLQQIKTK
                     YPNSLYMDDALYEIANTYFIMKQNSSAITAFKELVASKPNSSYAVKAYLKLGLIYFNE
                     KDYANSEKYYKMAYEMSPNSPEGNEAKDGLLDIVEETGDTDAADGVVPTSAIDQEKYN
                     YAKNKYDLGEYSGAAIAFSDYLREFPKGQYKTESEFYRGESYYKVEQYSKALPDFESI
                     IKGNKTKYLESALVRAGWIEYYQNEDYKSAYDYYDRLYDVADYKENTFVAMVGLLRTA
                     YFLNDFDQVIVNANRILGSDLVTNVEKIEAHYFAGQAHLGNNNIDKAYDAFSKAAALT
                     TNEIGVESRFHMADILFQKGKLDESKAKCQEIINDMPAYEEWIVRSYILLADISAAKG
                     DFAQAKATLNSIIDNYKGDPSLVELAKQKLEQIEQMEKSGRRKPSTDTNDDEELNFDN
                     N"
     gene            3132..>4568
                     /locus_tag="PLB46_10680"
     CDS             3132..>4568
                     /locus_tag="PLB46_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77028.1"
                     /translation="MLNINKYITIAVCVCGISMSTNHLQAQTNVGDEDVDVVSGYNPV
                     LADAVKENFNAELPENKTKPEPQEYNIPIEFYQIPYQPVKVKPIRLPDAKPTELENVY
                     VKAGFGTQFTPLAEVYLNSDRNKKYNYGLYGKYISSNGKVENQNYSDLRAGGSTNFYF
                     DDKYALPINAFYSRNTLQYYGYNPDDTSFKSEDVRQIFNNYGGSIGFHNIGDNPLNMD
                     FGLQLGVDGIRDINKNKEIHPFLTGWGEKKLENKSIAGAEVSYEYFSYSGVTEHDNSL
                     IGVKPYYKMITDQWSLNAGIETKVDKASNAYVLPDAVFSYDLIGDKFVFVAGWEGHLQ
                     VNSFANIVEENPFVKDTLVFKNTSINEIHAGLRGATYGNFSFMVKGYQKLAKDMPFYV
                     TDSVDTKRFDIIYADATIWGGNLELSYFDADRFRVTGSLNVFTFSEVDIIDKPYHRPT
                     LEWTINGQYKFNQKLSMEANIFGVNKSYA"
BASE COUNT         1629 a          679 c          883 g         1377 t
ORIGIN      
        1 ggtttacttt actgaaattc aattatgtta aaaaataaaa tacaatttgt aatttttctg
       61 ctactgatat gtgctttcag gcaagttcag gctcaacaat caatgattta taccgatttg
      121 tataaagatt acaaaaaggg gatggagtta tttgaaaatc agatgtactc cacagcacag
      181 gagttattta atacggttgg tgccattaaa cctgaaggga ctgacggaga aatcatcaca
      241 tttgtggcta atagtcagta ttatggcgcc atctgcgcta ttgagctgca gaaccctgat
      301 gcagaaaaac ggatgttaga tttcgttgaa aataatcccg gacatgtgtt gttgggttcc
      361 gcatacaatc agttgggtaa aatttattac aacaaaaaag aatataccaa cgctgttacc
      421 tggttcgaaa aagtagatgt taaagattta tcgcttgttg aacgcgattt atataaattc
      481 caattcggct atagcttatt taataaaaaa cgattcaccg aagccaaggt aatgtttgct
      541 caattaaaag acaaagcaaa cagcccttat tattatggcg caaattatta ttacggattt
      601 attcagcagg aggaaggcaa ttatgaaaat gcactgagct gtttcgaaat tgttaaagat
      661 attccaccat actcatcggt tgtgccttat tatatgacga atatttattt ccaacaaaag
      721 aaattcgaaa aggtaatatc atatgctgaa ccgctgatgg ctgataccaa actggtatat
      781 tatgcagaaa ttaacagttt attaggccag gcttattttt ataaagaaaa ttataaaaaa
      841 gctttaccgt atttaagcta ttatattgaa aaggctaaaa acattcgtga tgaagactat
      901 tatcagttag cctttacgca ttatcaaact aaagattata atgctgcgct tgcaacatta
      961 aaagaaactt ctgacgatga tgatacctta tatcagcaca tccaattttt atatggtaac
     1021 tgttatatcg agctcaataa aaaggatgaa gcgcgaaatg catttatgga agcatctcgt
     1081 attggggttg atgctgagat acatgaaagt gccttattta attatggtaa actcagttat
     1141 gagtcggcat tttatacagc tgctgtttca gccttacaag attatttgaa aaaatatcct
     1201 aaaggaaaga aaaaaacaga agcacaggaa ttgttggcca gctcgttatt aaatactaat
     1261 gactacgttg ccgcaatgga agttattgat aacattcctc aaaaaacacc aaagttaaaa
     1321 gaggcctatc aaaaagtttc ttattacagt ggtgtgcagt tgtttaacca aaaaaattat
     1381 aatgaagcag ataaaatgtt taatcagtcg ctggaaaatc ctatagatgc tgatttacag
     1441 gctgcttgtt atttttggat aggggaaatt cgttatacgg aaaataaata tgcattagct
     1501 attaataatc atgtaaaatt ccaggactta tctaaagtag ctaaagattt acctgaggaa
     1561 actaccattc cgtattctaa ctacacaatt gggtatagtt attttaaaca aaaatcgtac
     1621 agcaatgcat ccaactattt cagtaaagtg actacctcgc tcaaaacaag taaaactgat
     1681 atcacgcaaa ataatgttta tatagatgct gttttgagat taggcgattg ttatttcatg
     1741 acaaaagatt ataaaaaagc tgagcagaac tacagtaaaa ttattgataa caacagcaaa
     1801 ggtgttgatt atgccttgta tcaaaaaggg atgttgcagg ggttacaaaa taataacaac
     1861 ggtaaaatca gcacacttca gcaaattaaa accaaatatc caaattcatt atacatggat
     1921 gatgctttgt atgaaatagc caatacctat tttattatga aacaaaattc aagtgccata
     1981 actgcattta aagaattagt tgcttcaaaa cccaacagta gttatgctgt taaggcatat
     2041 ttgaaattag gattaatcta ctttaatgaa aaagattatg ctaattcaga gaagtattat
     2101 aaaatggctt atgaaatgtc gccgaattca ccagaaggaa atgaagctaa agatggttta
     2161 ttggatattg tggaggaaac cggtgatact gatgctgcag atggtgttgt gccaacttct
     2221 gcaattgatc aagaaaaata taattatgct aaaaacaaat atgatttagg tgaatacagt
     2281 ggcgcagcaa tagcattttc agattatcta cgtgaattcc cgaaaggaca atataaaacg
     2341 gaatcggaat tttaccgcgg tgaatcctat tacaaagtag aacaatacag caaagcatta
     2401 cctgatttcg aatcgataat aaaaggtaac aaaacaaaat atctggaatc tgctcttgtt
     2461 cgcgctggat ggattgaata ttaccaaaat gaagattata aatcggcata tgattattat
     2521 gacagattgt atgatgttgc tgattataaa gaaaatacat tcgtggctat ggttggtttg
     2581 ttgcgaactg catatttcct caacgacttt gaccaggtaa ttgttaatgc aaacagaatt
     2641 ttaggtagcg accttgttac caatgtcgaa aaaattgaag cgcattattt tgcgggacaa
     2701 gcacatttag gtaataacaa tatcgataaa gcctacgatg cgttttcaaa agcggctgcc
     2761 ttaacaacca acgaaattgg tgttgagtcg agattccata tggctgatat cttattccaa
     2821 aaaggtaaat tggatgagtc gaaagctaaa tgccaggaaa taattaatga tatgcctgct
     2881 tatgaagaat ggattgtgcg tagttacata ttgttagctg atataagtgc agctaaagga
     2941 gattttgctc aggcaaaagc aacactaaac agtattattg ataattataa aggtgatcct
     3001 tcacttgttg aattggctaa acaaaaatta gaacaaatag aacaaatgga gaaatcagga
     3061 agacggaaac catcaacaga taccaatgat gatgaggaat taaatttcga taacaactaa
     3121 agaaaacgac aatgttgaac ataaataaat atataacaat tgcagtttgt gtttgtggaa
     3181 tttccatgag cacaaaccat ttacaggcac aaactaatgt aggtgacgaa gatgtggatg
     3241 tggtgagtgg ttacaatcct gttttagcag atgcggtaaa agaaaacttt aatgcggaat
     3301 tgccggaaaa taaaacaaaa cctgagcctc aggaatacaa tattcctatt gaattttacc
     3361 agattccgta tcagcctgta aaggttaaac ctattcgttt acctgatgca aaaccaactg
     3421 agctcgaaaa tgtatatgta aaagccggat ttggaacaca atttacccca cttgctgagg
     3481 tatatctgaa cagcgataga aataaaaaat ataattatgg tttatatggt aaatatattt
     3541 cttcgaacgg taaggtagaa aatcaaaatt atagcgattt aagagcgggt ggaagtacta
     3601 atttttactt tgatgataaa tatgctttgc cgataaatgc attttattcc agaaatacgc
     3661 tacaatatta tggttataat cctgatgaca cttcatttaa atccgaagat gtacgccaga
     3721 tttttaataa ctatggcggt agtattggat ttcataatat cggtgacaat ccgttaaaca
     3781 tggattttgg attacaattg ggtgttgatg gcattcgcga tattaataaa aacaaagaga
     3841 ttcatccatt tttgaccggt tggggcgaaa agaaattgga aaacaaaagt attgcaggtg
     3901 cagaagtaag ttatgaatat tttagttatt caggcgtaac cgaacatgac aattcactta
     3961 ttggtgtaaa accttattat aaaatgatta ccgatcagtg gtcactgaat gccggtatcg
     4021 aaacaaaggt tgacaaagcg agtaatgctt atgtgttgcc tgatgcggta tttagttatg
     4081 atttaattgg cgataaattt gtttttgttg ccggatggga aggtcattta caggtaaata
     4141 gttttgccaa tattgtggag gaaaatcctt ttgtaaaaga tacacttgta tttaaaaaca
     4201 cttctattaa cgaaattcat gcaggattaa gaggtgctac ttatggtaac ttcagtttta
     4261 tggttaaagg ttaccagaaa ttggctaagg atatgccgtt ttatgtaacc gattcagtgg
     4321 atacaaaacg atttgatatt atatatgctg atgccaccat ttggggtgga aatctggagt
     4381 tgagttattt tgatgctgac cgttttcgtg taacagggtc gttgaatgtg tttactttct
     4441 cggaagtgga catcattgac aagccttatc atcgtccaac acttgaatgg acgattaatg
     4501 ggcagtacaa attcaaccag aaactcagta tggaggcaaa tatcttcggg gtaaataagt
     4561 cttatgcg
//
LOCUS       DAORKW010000274         4559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_872_length_4559_cov_3.576821, whole genome shotgun sequence.
ACCESSION   DAORKW010000274 DAORKW010000000
VERSION     DAORKW010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_872_length_4559_cov_3.576821"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLB46_10685"
     CDS             complement(<1..338)
                     /locus_tag="PLB46_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77029.1"
                     /translation="MSTFEFTTAIIDETVFLLDDAAKLTISAKNANDTTVINLNKVST
                     VIVFAEENADESSNTTIIAMHKMAAALKLATSDYGTINLKQHPEFKIKQIFANTGVQK
                     LICLGVDLKD"
     gene            407..1486
                     /locus_tag="PLB46_10690"
     CDS             407..1486
                     /locus_tag="PLB46_10690"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRF77030.1"
                     /translation="MKVAVNPAYEIDIHLSPVSTIDSVDFENLQFGKVFTDHMFEADY
                     VDGKWTNCAIKPLTKLQLHPATSALHYGQAIFEGMKAFKNQEGKATLFRPDANLLRLN
                     ESCRRMAIPEVPEELFMNALFNLVRIEQDWIPNTDDGSMYIRPYIFATDDFVGIKPSD
                     TFKLVIFCCPVARYYQKPVRVYMQEEYVRAFPGGTGAVKAAGNYAATMMPLQQIREKG
                     YDQMLWIDGVTHNRLQEIGTMNVFVQIDDTIITIPTDEGTILKGITRDSCIELLKAEG
                     FKVEIRDVTIQELLDAHKTGRLHDAFGTGTAATIAPIGVIGYKDVAYELPPVEGRKAS
                     NWLKQEMWSIRKGISEDRFGWMWAV"
     gene            complement(1490..3466)
                     /locus_tag="PLB46_10695"
     CDS             complement(1490..3466)
                     /locus_tag="PLB46_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF77031.1"
                     /translation="MLLVFSNTLVNAQEVVWAKTVTNFSSEKSNKAFGAIQVIGKPNR
                     YPNPAFSTCAWSPDYNESRSEEFIEVKFEHTVIGKQILVVENNNSGHIKSIVIVFEDN
                     TTKQVYTRTAAAISGPGRLFVAGIKENVRPILGVKLILDQPELMNTYQIDAIGISTST
                     APPLLTINTTPDPLNNKAISLGPNINSQFDEVYPIISPDGSTLYFDRKVHPGNIGSAK
                     NDDIWVAQRLDGIWSEAKNIGPPLNNENHNFICSISPDGNTALVGNVYLPSLGDGAGV
                     SLTHKKIDGWTLPQPVKINSFSNLNQYNEFCISGDGSIMVMSLETASGKGLLDLYVSF
                     LQADGSYSTPKNFGPAINTAGNEMTPFLAADGKTIYFSSNGFPGYGNQDIFMAKRLDN
                     TWTNWTEPLNLGAEVNTPEWDVYYSIDAKGENAYFSSSKTNGTNLDIFSIKLPPQAKP
                     EDVLWLKGVVSDRITSLPINADLTIKTTSEPENFGYTNSSISSGYALILQQFGSYEIQ
                     ISADGYYVNDTVITIDSLIGFDEIIRNFSLIPRKEGLIIEMKNILFKVNSSVLEDSSY
                     QEIDKVARFMKSNTGVAIEIRGHTNGLCDDDYCNMLSEKRAKAVVDYLIKNGIDRNRL
                     TYKGLGKTQPVADNKTVAGRQANQRVEFMITKTE"
     gene            complement(3504..>4559)
                     /locus_tag="PLB46_10700"
     CDS             complement(3504..>4559)
                     /locus_tag="PLB46_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF77032.1"
                     /translation="ASMEMAPFLAPDGKTMFFSTNGLPGYGDQDVYVSTRLDSTWRNW
                     TKPENIGTLVNTKGFEAYFTIPDTADFAYFSSTGSNMLNADIYRIPLKEIKEIEDSVV
                     AEILETKLGSDSLGTTPSDPTVETDVIFFEEDVYVPTKEELNVILTNEILLWGTIYDA
                     TTDIAINADLTFLLNDYDSDPVALSTLNNTYRLKVTDSVNYKVTIIREGYLPLETNIN
                     IEDFRMQKVKRIDFRLTPLRKGEKIILDNLYFDANKSIIKPESFEELNRLYDFLKANP
                     GTTIEIGGHTNGLCSETYCEKLSLNRANAVREYLISKGIDGLRITTFGYGSKQPIDTN
                     STPEGRKRNQRVEITFK"
BASE COUNT         1330 a          866 c          893 g         1470 t
ORIGIN      
        1 aagtctttta aatctactcc taagcatatt agtttttgaa cgcctgtgtt ggcaaaaatc
       61 tgtttgattt tgaattcagg atgttgtttc aggttaattg tgccatagtc ggaagttgct
      121 aactttaagg cagcagccat tttatgcatg gcaattattg tggtattgct actctcgtct
      181 gcattttctt cggcaaaaac gattactgtt gaaactttat ttaggttaat aacagttgta
      241 tcattggcat tctttgccga tattgttagc tttgcggcat catcaagcaa aaacactgtt
      301 tcatcaatga ttgcagtggt aaattcaaaa gttgacatgc gcgatgaaat tagttaagca
      361 gtaaaattat caatcatttc attcataaaa acaaggtttt caacaaatga aagtagcagt
      421 aaatccggca tacgaaatag acattcactt atcacctgtt tctactatcg attcagttga
      481 ttttgaaaac cttcaattcg gtaaagtatt taccgatcat atgtttgaag cagattatgt
      541 ggatggcaaa tggacgaatt gtgccataaa gccattaaca aagctgcaat tacatccggc
      601 tacatcggct ttgcactacg ggcaggcaat ttttgaggga atgaaggcat tcaaaaacca
      661 ggaaggaaaa gctacattgt tccgccctga tgccaacctg ttgcgtctaa atgagtcatg
      721 ccgcagaatg gctattcctg aagttcctga ggagttgttc atgaatgcac tgtttaattt
      781 agtgcgtatt gagcaagact ggattccaaa taccgacgac ggttcaatgt atattcgacc
      841 atacattttc gccaccgacg actttgttgg tataaaacct tcagacacct ttaagttggt
      901 gattttttgc tgccctgtag cacgttacta tcaaaaaccg gtacgtgttt atatgcagga
      961 agaatatgtt cgtgcatttc cgggtggaac aggagctgta aaagctgccg gtaactatgc
     1021 tgctaccatg atgccattgc aacaaatacg tgaaaaaggc tacgatcaaa tgttatggat
     1081 agatggtgta acacataatc gcctccagga aatcggcact atgaacgttt ttgtgcagat
     1141 tgatgacaca attattacca ttccaactga tgaaggaacg attttaaaag gaattacccg
     1201 tgattcttgc atagaattgt tgaaagcaga aggatttaaa gttgaaattc gcgatgtaac
     1261 tattcaggaa ttactcgatg cgcataaaac cggacgttta cacgatgcat ttggcaccgg
     1321 aaccgccgct acaattgcac ctattggtgt aattggttat aaagatgttg cttacgaatt
     1381 gccgcctgta gaaggacgca aagcatctaa ttggttaaaa caggaaatgt ggagtatcag
     1441 aaaaggtatt tcagaggacc gttttggctg gatgtgggca gtgtaatggc tactccgttt
     1501 tggttatcat aaattcaacc cgctgattgg cttgtcttcc ggcaacagtt ttattgtcgg
     1561 caaccggttg tgtttttcct aagcctttat atgtaagtct gttacggtca atgccatttt
     1621 ttattaaata gtctacaaca gctttagcgc gtttttccga taacatattg caataatcat
     1681 catcacaaag tccgttggtg tgccctctga tttctatggc aacacccgta ttacttttca
     1741 taaaacgagc aactttatct atttcctgat aggaggaatc ttctaataca ctgctattta
     1801 ccttaaacag gatgtttttc atttcaataa tgagtccttc cttgcgtgga atcaaactaa
     1861 agtttcggat gatttcatca aaacctatga gagaatcaat agtaatcaca gtatcattta
     1921 cgtaatatcc atcagctgaa atttggatct cataactacc aaattgttgt agtataagcg
     1981 cataaccact tgaaatagaa gaatttgtat atccaaaatt ttccggttct gaagttgttt
     2041 ttatggttaa atctgcgttt attgggagtg aagtaattct atcgctaacc acccctttta
     2101 accataaaac gtcttcaggt ttagcctgag gtggcaattt aatgctaaaa atatcgaggt
     2161 tagtaccgtt agttttactt gaactaaaat aggcgttttc acctttagcg tcaatgctgt
     2221 aataaacatc ccattccggg gtattgactt cggcacctag gttaaggggt tcagtccagt
     2281 tcgtccaggt gttatccagt cgttttgcca taaaaatgtc ctgattacca taaccgggaa
     2341 atccgtttga tgaaaaataa atggttttgc catcagctgc taaaaatggt gtcatttcgt
     2401 ttcctgcagt attaattgcc ggaccgaaat tttttggcgt agagtaggaa ccgtccgctt
     2461 gcaaaaaact cacgtaaagg tctaataaac ccttaccgga agcagtttcc agcgacataa
     2521 ccataatact gccatcacct gaaatacaaa attcgttgta ttgattcagg ttgctgaaac
     2581 tgttgatttt aaccggttgg ggtaatgtcc agccatctat ttttttgtgc gttaatgata
     2641 caccagctcc atcacccaag gaaggtagat aaacgttgcc tactagggct gtgttaccat
     2701 cagggctgat agaacatata aaattgtgat tttcattatt cagcggtggg ccaatgtttt
     2761 tagcctcaga ccaaatacca tccaaccgct gcgccaccca tatatcgtca ttttttgcac
     2821 tgcctatatt accgggatgt actttgcggt caaaataaag ggtgctgcca tccggtgaaa
     2881 tgataggata aacttcatca aattgagaat taatgtttgg ccctaaggaa attgccttat
     2941 tgttgagtgg atcaggagtt gtatttattg ttaataaagg aggtgctgta cttgttgaaa
     3001 taccaatagc gtcaatttga taggtattca tcaattcagg ttgatctaaa attaatttaa
     3061 ctcccagaat tggtcttaca ttctccttaa tccctgccac aaatagcctt cctggtcccg
     3121 atatagccgc tgcagttcgt gtataaacct gtttggtggt gttatcctcg aaaacaataa
     3181 cgatagattt aatgtggcct gaattattgt tttcaacaac cagaatttgt ttacctataa
     3241 cggtatgttc gaatttgact tcaataaatt cttcagacct ggattcattg tagtcgggag
     3301 accaggcgca ggtactaaaa gccgggttag ggtagcggtt gggttttccg attacctgaa
     3361 ttgctccaaa agccttattg cttttttcgg aggagaagtt cgttacagtt tttgcccata
     3421 ctacttcttg tgcattcacc agcgtattgc tgaataccaa tagcaagata aaccatacga
     3481 acttatgata tctagtttga gccttactta aaggtgattt ccacgcgctg attacgtttt
     3541 ctaccttcag gtgtgctgtt ggtatcaatt ggttgtttag aaccatatcc gaaggtggta
     3601 atgcgcaagc catcaattcc tttgctaatg agatactctc taactgcatt tgctctattt
     3661 aaactgagtt tttcgcaata agtttcacta cacaacccat ttgtatgtcc accaatctca
     3721 atagttgtac caggatttgc tttaaggaag tcatataatc tattcagttc ttcaaacgac
     3781 tctggtttta ttatcgattt attagcatcg aaatataagt tatcgagaat aattttttct
     3841 cctttgcgca gtggtgttaa acggaagtca atgcgtttaa ccttttgcat tctgaaatct
     3901 tcaatattga tatttgtttc cagaggcagg taaccctctc tgataatggt tactttataa
     3961 tttacactat cggttacttt taaacgataa gtgttattca aagtggacaa agctacaggg
     4021 tcgctatcgt aatcgttgag caaaaaggtc aaatctgcgt taattgctat atcggtggta
     4081 gcgtcataaa ttgttcccca caataaaatc tcattagtta atatgacatt gagttcctcc
     4141 ttggttggta cataaacgtc ttcctcaaaa aatataacat ctgtttcaac agttggatca
     4201 gatggggtag tccccaaaga gtctgatcct aatttagttt caagtatttc tgcaacaaca
     4261 gagtcttcaa tttctttaat ttcttttaat ggaatacgat aaatatcggc attcagcata
     4321 ttgctaccgg tagaagaaaa ataggcaaaa tcagctgtat ccggaatggt aaaataggcc
     4381 tcgaaacctt ttgtattaac taatgttccg atattttcag gtttagtcca atttctccag
     4441 gtactgtcaa gtcttgtgga aacatataca tcctgatcac cataacctgg tagtccattg
     4501 gttgaaaaaa acatggtttt accatcagga gcaagaaacg gtgccatttc cattgaggc
//
LOCUS       DAORKW010000275         4558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_873_length_4558_cov_4.404175, whole genome shotgun sequence.
ACCESSION   DAORKW010000275 DAORKW010000000
VERSION     DAORKW010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_873_length_4558_cov_4.404175"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..552)
                     /locus_tag="PLB46_10705"
     CDS             complement(220..552)
                     /locus_tag="PLB46_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77033.1"
                     /translation="MKMVTLIAALFLTTNLFAGNDETCSSNSIKENIIHLIETAPPFS
                     DFDLEGIVTIQFEVDDNHHIHVKQVSSGNTLLASHVMSSLQNKLLSCDCVMPDTTYVL
                     RLQYVQYS"
     gene            complement(764..1108)
                     /locus_tag="PLB46_10710"
     CDS             complement(764..1108)
                     /locus_tag="PLB46_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77034.1"
                     /translation="MKNLFTIAAILLSTSLFAGNENKNVSDKDKTSVIDNISNLVGDE
                     TILSQLGVKGQADVTITVDENGLVHVASVQTNDYLLAYHIKQTVEDAKMIVNDSLVGK
                     TVSFIMNVVQSK"
     gene            complement(1233..2447)
                     /locus_tag="PLB46_10715"
     CDS             complement(1233..2447)
                     /locus_tag="PLB46_10715"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRF77035.1"
                     /translation="MDLLRRNIPEKVVFGMICFIAMLIPFHKTLASPVLLLSFITILL
                     SLNYAYKLERLLENKRYLFFASIFLLFVFGYFFSEDKHTAYRDLEVKFYLLAIPVFYV
                     LIQPFTIKERKLVTTLYVIACLLFILVAFSIAFYDFIVLGENHFYYKDLLAFTYLHPS
                     YAGMFQAFAAVIIVMHLLTNWFEIPKKWRFIYLALLAIITLFIFLLTAKMAIGSIFIL
                     LSVAFYLLGKQILGKSKTIFIIVLGNVVAIAIMLSLPYTRERLKLLFTYNEVSYENSV
                     NSRQEIWKAAIEVAQNNWLVGTGTGDAEKELIAAYQRNNFQLGADEKYNAHNQYLQIL
                     VETGVVGLILFIAYLIWCLRLAIRSRNHIYLVFLLLWIVNIVTESMLETQSGVVFFSF
                     FNVLLAMDYSRK"
     gene            complement(2459..3895)
                     /locus_tag="PLB46_10720"
     CDS             complement(2459..3895)
                     /locus_tag="PLB46_10720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRF77036.1"
                     /translation="MCIHVVTAQKKYDYRLLFRRYILITVDVLLILTALFLYIRFFHP
                     NALQSYHTFLDNVLWIAVIVVLWFFYSYLFNLYKLANVDRIRATVKNTVLTAALTALT
                     YLFMPFLSPTFPESRFPAFVLMGAMIVLVLLWRLCYAAFFIHPILTKKAIVIGAGYTG
                     REIVRTLLHNPAIYHNTGYKIYGYIDDDATKQGKIYDELKVLGDGNDLFKYARRLDID
                     EIIMAIPEQEHLSAALHRALIDCENAGIVVEHATKIYEEQTGKVMIKIKANEYHLTNP
                     YSIVHKDNFYAFFNRVMNISCALFAGVFFLVVLPFVWLGNLLFSRGPLFYTQERVGEN
                     NKIFHIIKFRTMIVDAEKNSGPQFAQKSDPRITKVGNILRKTRLDEFPQFWNILRGDI
                     NLIGPRPERQYFVDELSKQIPFFKLRNAVKPGLTGWAQVKHHYASDFDDTLIKLQYDL
                     YYIKHRSLLLDFMIIFKTIAVMVKFKGT"
     gene            complement(3908..>4558)
                     /locus_tag="PLB46_10725"
     CDS             complement(3908..>4558)
                     /locus_tag="PLB46_10725"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelL-related redox protein"
                     /protein_id="HRF77037.1"
                     /translation="LFIRFIILNHLIWLVPNAIVLYKVYRRSFETDEMLMDTFSSEQY
                     PLSLFDTTDGRNLGEMHEEKPLMLVFLRHFGCPFCKESLLQLAEHRNQLEAEGIGIVL
                     VYMVDDNTAQAYLSEYGLNDVAQVSDPEEIFYKSFRLKRGSFLQLFGLKVWVRWVELA
                     FRKKLFNTKPAGDVAQMPGIFLLQDGKVVKEFVHKSVADSPDYSFFLNYRENSSYN"
BASE COUNT         1579 a          861 c          752 g         1366 t
ORIGIN      
        1 gattaaatgt aggtgtcaca tataatgaaa catcctcctg aactgccaca gttggtgcca
       61 taacccgtaa gtatacggta taacctgaac ctacaccgat aataccgaga aatgcaatga
      121 ttactgcaac tataataacc gttttccttt taatagccat ttggttatgt tattatgcaa
      181 aaataacaaa gctataggta ttctcaaggc ggttttcaat taactatact gcacatattg
      241 taatcttaat acgtaggttg tatcgggcat cacacagtcg cagctgagta atttattttg
      301 taaagaactc atcacatgac tggcaagcaa agtattgcca ctgcttacct gcttaacatg
      361 aatatggtgg ttgtcatcaa cttcaaattg aatggttaca attccttcca aatcaaaatc
      421 agaaaaaggc ggtgcagttt caatcagatg tataatattt tctttgatcg aattagatga
      481 acatgtttca tcgttgccgg caaataaatt ggttgttaaa aataatgctg caattaaggt
      541 taccattttc ataatacata ttttttatta atgagataaa tacattcgtt ataaattcac
      601 aatgcaaagg tctaattgaa ttgttactac tgtatgagga gaaaatgaca aaaaggttac
      661 attaatattg attaaaatgt gtgaatatta tttaacaaat attaataagc ataaaaaaag
      721 gccccctttc aggaggcctc acgcaaacga agtggatgac atgttacttc gattgcacta
      781 cgttcatgat aaacgaaact gttttaccta ctaacgaatc attaacaatc atttttgcat
      841 cttcaacggt ttgtttaata tggtatgcca acaagtaatc atttgtttgt actgaggcaa
      901 catgcactaa accattttca tcaacagtaa tagttacatc tgcttgtccc ttcacaccta
      961 attggcttaa tattgtttca tccccaacta aattagaaat gttgtcgatt actgaagttt
     1021 tatctttatc tgaaacattt ttgttttcgt ttcctgcgaa taaagaagtg cttaagagga
     1081 tagctgcaat tgtaaataag tttttcataa tatctgattt tggagtgatt attaaatttg
     1141 ttttaattaa cgttctgaac attaggcgga tgaccccaca taaaagttac aggaaggcta
     1201 aaaaaagttg tgaaaaagtg gttaaaggaa ccttattttc ggctgtaatc catagccagc
     1261 aagacattga agaaactaaa aaaaactaca ccgctttggg tttccagcat cgattcggta
     1321 acaatattaa caatccataa caaaagaaac acaagataaa tatggttccg ggacctgatg
     1381 gcaagtcgca aacaccagat taaataggct ataaacagga taagacctac aacccccgtt
     1441 tctaccaaaa tttgtaaata ttgattgtgg gcgttatact tttcgtcagc acctagttga
     1501 aaattattgc gctgataggc agcaattaat tccttttctg catcaccggt tccggtacca
     1561 accagccaat tgttttgcgc cacttctatg gctgctttcc agatttcctg tctggaattc
     1621 acactatttt cataactcac ctcattataa gtaaacaata gttttaatct ttcgcgggta
     1681 taaggtaaac ttaacatgat ggcgatagca acaacattac ccaatacaat aatgaagata
     1741 gttttcgatt ttcccaaaat ttgtttgcct aacaaataaa aggcaacact taataatata
     1801 aatatactgc ctattgccat ttttgcagta agcaggaaaa taaacaaggt aataattgcc
     1861 aatagtgcta aataaataaa tcgccatttt ttgggtattt caaaccagtt ggtgagcaaa
     1921 tgcataacaa taatcacagc agcaaaggcc tgaaacatgc cagcatagga tgggtgtaaa
     1981 taagtaaacg ctaataaatc tttgtagtaa aaatgatttt ctcccaatac tataaagtcg
     2041 taaaaggcta tactaaacgc aaccaggatg aataataaac atgcaattac atataaggtg
     2101 gtaaccagtt ttcgttcttt aatagtaaac ggctgaatta aaacataaaa aacaggaata
     2161 gccaataaat aaaatttaac ttccaaatcg cggtaggcgg tatgtttatc ttcagaaaag
     2221 aaataaccaa aaacaaacaa caggaaaatg gatgcaaaaa ataaatacct tttgttctct
     2281 aacaaccttt ccagtttata ggcataattc agacttaata aaatggtaat aaacgataac
     2341 aataaaacag gtgatgccag ggttttgtga aatggtatga gcatggcaat gaaacagatc
     2401 atgccgaata ccaccttctc aggaatattt ctccttaata aatccaagct gttgtgaatt
     2461 aagtgccttt aaatttaacc atcaccgcaa tggtcttaaa gataatcata aaatcgagta
     2521 gaagtgaacg gtgtttaata taatacaaat catactgcaa ttttatcagg gtatcatcaa
     2581 agtcggaagc ataatgatgt ttcacctgcg cccagccggt taggcccggc ttaacggcat
     2641 tgcgcaactt aaaaaaagga atttgtttac tcagttcatc aacaaagtac tgccgctcag
     2701 gccttggtcc tattaaatta atatcacctc ttaaaatatt ccaaaactga ggaaactcat
     2761 ctaatcgtgt tttgcgcaat atattgccca ccttggtaat tcggggatca cttttttgag
     2821 caaactgtgg accgctgttt ttttctgcat ccacaatcat ggtcctgaat tttataatat
     2881 gaaatatttt attgttttcc cccacacgtt cctgtgtgta aaataacggg cctctgctaa
     2941 acaataaatt acccaaccag acaaaaggca acacaactaa aaaaaatacg cctgcaaaaa
     3001 gtgcacagct gatattcatt acgcgattaa aaaaagcata aaaattatcc ttatggacaa
     3061 tactgtatgg attggttaaa tgatattcat tggctttaat tttaatcatc acctttccgg
     3121 tttgctcttc atatattttg gtagcatgct ccacaactat gcccgcattt tcgcaatcaa
     3181 tcaacgcgcg atgtaatgct gcagaaagat gttcctgttc aggaatagcc ataataattt
     3241 catcaatatc caatctgcgt gcatatttaa acaagtcgtt tccgtcgcct aacactttta
     3301 attcatcgta aatttttcct tgtttcgttg catcatcatc aatataacca taaattttat
     3361 atccggtgtt atgatatata gcaggattat gtaataatgt tcggacaatt tctctgccgg
     3421 tatatcccgc tccgataacg attgcttttt tagttaagat gggatgaata aaaaatgccg
     3481 cataacatag ccgccacaac aacactaaaa caatcatagc ccccatcaac acaaaagcag
     3541 gaaaacgact ttctggaaac gtgggactta aaaacggcat aaataaatac gtcaatgcag
     3601 ttagcgctgc agtgagtacg gtatttttta ctgttgccct aatcctatcg acattggcca
     3661 gtttatataa attaaataaa taagaataaa aaaaccacag cacaacaatc acagcaatcc
     3721 ataaaacatt atctaaaaat gtatggtacg attgtaatgc attggggtga aaaaatctga
     3781 tgtacagaaa taatgcggta agtatgagca atacatcaac cgttatcaga atatatcttc
     3841 tgaataacag tctgtagtca tatttttttt gagcagtcac aacgtgaatg cacaaaaata
     3901 acacttgtca attgtaactg gaattctctc tgtagttaag aaagaagctg tagtccggtg
     3961 aatcggcaac tgatttgtgc acaaactctt tcactacttt accatcttgt aacaggaaaa
     4021 taccgggcat ttgagctaca tcaccagccg gtttggtatt aaatagtttt ttgcggaatg
     4081 ctaattctac ccaacgaacc cacactttta agccaaacaa ctgtaaaaag ctgccgcgtt
     4141 tgaggcggaa cgacttatag aatatttctt caggatcgct aacctgagca acgtcattta
     4201 ggccatattc actcaaatat gcttgagcag tattgtcatc aaccatatat acaagcacta
     4261 ttccaatacc ttcagcttcc agttgattgc gatgttcagc aagttgtaaa agggattctt
     4321 tacaaaaggg gcatccaaaa tgacgcaaaa acaccaacat caatggtttt tcctcatgca
     4381 tttcccctaa attgcgacca tctgtagtgt caaacagcga caatggatat tgttcactgc
     4441 taaaggtatc catcaacatt tcatcagtct cgaaacttct gcgatatacc ttgtaaagca
     4501 ctatggcatt aggtaccagc caaataaggt ggtttaaaat gataaatcgt ataaagag
//
LOCUS       DAORKW010000276         4540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_878_length_4540_cov_4.341360, whole genome shotgun sequence.
ACCESSION   DAORKW010000276 DAORKW010000000
VERSION     DAORKW010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_878_length_4540_cov_4.341360"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="PLB46_10730"
     CDS             complement(<1..1035)
                     /locus_tag="PLB46_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRF77038.1"
                     /translation="MNRLPSHEVLPFLLLVATLLLAGRLLGELFRKMKLPQVMGELVA
                     GILLGPSCLQMIAPDFYQSFFVDPKNAGIAFDGLARIGILLLLFVAGQEINLKTIRAK
                     ANAAAAISLSGIAIPFIVGFAATWFFTPLLFPGGVVDKTASSLFMGTALSITALSVLA
                     KILIDIKQLKSKFGNLMMTAAMIDDFIGWMLFSLVVSVANLESESSFEGWQVMFMVIG
                     FAAFILTVGRKLIHYSFIFSNKHLSSGGGTLSLAIIFCILGAIFTEWLGLHAIFGAFL
                     VGIAVGDTEEFSGKNREMLHDIVTYIFAPLFFVSIGFRVNFIENFDGVVIAFVLLIAV
                     FGKLLGGFLGG"
     gene            complement(1208..1864)
                     /gene="fsa"
                     /locus_tag="PLB46_10735"
     CDS             complement(1208..1864)
                     /gene="fsa"
                     /locus_tag="PLB46_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRF77039.1"
                     /translation="MKFFIDTADLNEIKEAAALGILDGVTTNPSLMAKVGITGADNIK
                     KHYVDICNIVDGDISAEVISVDFDGMVREAKELSALHPNIVVKIPMIKEGIKAIKWCT
                     DNGIRTNCTLVFSAGQAILAAKAGATYLSPFIGRVDDMNWDGIQLIEQIVHIYRIYGF
                     ETEVLAASIRNPLHIVRCAEVGADVVTCPLSSILGLFKHPLTDIGLAQFLADYKKGNG
                     "
     gene            2223..3851
                     /locus_tag="PLB46_10740"
     CDS             2223..3851
                     /locus_tag="PLB46_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRF77040.1"
                     /translation="MDITFNKNEDALKLLVTTMKQRLAKVAEGGGKKSIDKLHEKGKL
                     HARERIKLLFDQDKPCIEIGAFTGYNMYQEHGGCPSGGVVVKIGYVAGRQCIVVANDA
                     TVKAGAWFPITGKKNLRAQEIAIENKLPIIYLVDSAGVYLPMQDEIFPDKENFGRIFR
                     NNAVMSSMGITQIAAIMGSCVAGGAYLPIMSDEAYIVKNTGSIFLAGPYLVKAAIGED
                     IDAETLGGAITTTEISGICDYAAENDEDCLTHIRKTMDKIGAKPKAGFDRVASKPPVL
                     KEEDIYGIMPEEITKQYDVLDIIKRLVDDSAFDEYKPAYGKTIVCGLGRIDGWSVGIV
                     ANQRKVVKNKKGELQIGGVIYNDSADKAARFIMNCNQKKIPLLFLQDVTGFMVGSKSE
                     HAGIIKDGAKLVNAVANSVVPKFTVIIGNSFGAGNYAMCGKAYDPRLIVAWPNAKIAV
                     MGGAQAGKVLLQIQVASLKAKGEVITAEKEKELLDHILNHYNETMDPYYAAARLWIDG
                     IIDPLDTRKWVSMGIEMANNNDEMRAFNPGVMQV"
     gene            3971..>4540
                     /locus_tag="PLB46_10745"
     CDS             3971..>4540
                     /locus_tag="PLB46_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRF77041.1"
                     /translation="MKKWLGFVFIITTLSAIAQNKIIPAPLSVKPAPGYFTFDPTTYL
                     LGNDQVAYKDGYVIKDFLIKYYNLPLKTATKTIGVGKHIALKYDSTLAVSDGGYHIMI
                     SPENITITGKNNGGVLYGLLSLMQLIEKTGNTYQIPCGEITDYPQFQYRGMHLDCARH
                     FFDVAFIKKYIDYLAMYKMNTFHWHLTDDQ"
BASE COUNT         1523 a          830 c          895 g         1292 t
ORIGIN      
        1 acctcccaaa aatccgccaa gtaattttcc gaacaccgca attaataata caaaagcgat
       61 taccacacca tcgaaatttt caataaagtt tactctgaaa ccgatggata caaaaaataa
      121 aggcgcaaaa atataggtga caatatcgtg taacatttcc cggtttttcc cgctgaattc
      181 ttctgtgtca ccaacggcaa tgccgactaa aaatgcgccg aatatggcgt gtaaacctaa
      241 ccactcagta aatatcgcac cgagaataca aaatataatt gccagcgata aagtaccacc
      301 accactggat aaatgtttat ttgaaaaaat aaatgagtaa tgaattaatt ttcttccgac
      361 agtcaaaata aatgcagcaa atccaattac cataaacata acctgccatc cttcaaaact
      421 gctttcactt tctaaattgg caacacttac cacaagagaa aataacatcc aaccaataaa
      481 atcatcaatc atcgctgctg tcatcatcaa attgccgaat ttacttttca gctgcttgat
      541 atcaattaat atttttgcca acacggataa agcggtaatc gacaatgccg tacccataaa
      601 aagcgaagaa gcagttttgt ctactacacc accgggaaac aacaagggtg taaaaaacca
      661 ggttgccgca aaacccacaa taaatggaat ggctattcca gataaactga ttgctgctgc
      721 tgcatttgcc tttgcacgta tggttttcag attaatttcc tgaccggcaa caaataacaa
      781 taacaaaatc ccaattctag ccagaccatc aaatgcaatt ccggcatttt tagggtcaac
      841 aaaaaatgac tgataaaaat cgggagctat catctgcaaa caagatggtc ccagtaaaat
      901 accggcaact aattcgccca ttacctgagg caatttcatt tttctgaata actcgcctaa
      961 caaacggccg gcaagcaata gggttgctac aagcaataaa aatggcaata cctcgtggga
     1021 aggtaatcgg ttcataatag atgggttata aggttactca atgtaaaact tcattctccg
     1081 gctaaggatg caaggctaat tgcgaaatga aatgctactt ttattctatt gatttaatag
     1141 aataaggccc caaagctaag ctttgaagcc ttattattaa aagaatttta gaaattatcg
     1201 gataaagtta accgttacct tttttataat ccgctaaaaa ctgcgctaat cctatgtctg
     1261 tcaatggatg tttgaataaa ccaagaattg agctaagcgg gcaagtaact acgtcggcgc
     1321 caacttcggc acaacgcaca atatgtaatg gattgcggat agaagctgct aatacttctg
     1381 tttcaaaccc gtaaatgcga taaatatgta caatttgttc aatcagctga ataccatccc
     1441 agttcatgtc atctacacga ccaataaacg gtgaaaggta ggtagcacct gcttttgctg
     1501 ccaaaattgc ctgtccggct gaaaatacca gggtgcaatt tgttctgatg ccattatcgg
     1561 tgcaccattt gattgcttta atcccttctt taatcatagg tattttcacc acaatattgg
     1621 gatgcaaagc agaaagttct ttcgcttcgc gcaccatacc gtcgaaatca acggaaataa
     1681 cttccgcact aatatcgcca tcaacgatat tacaaatgtc gacataatgt tttttgatat
     1741 tatcagcacc tgtgatgcca actttagcca ttaatgaagg attggtggtt acaccgtcga
     1801 gaattcccaa agctgccgct tcttttatct cgtttaagtc tgctgtatca ataaaaaatt
     1861 tcatagtaat taaatcgttg ccgcaaaatt agcaggtaat cttgtaaaat cgcattgggt
     1921 tttcaacaaa taacgggcaa gtagtgaaga aacttctgaa taaaagggga accgtagtcg
     1981 aaaaataaaa aagggcaagt gatctacatc gcaccgctac aaccacctac ccttgctccg
     2041 gtcagggcct gggggattca gcaggagctg gtcgtatagg acttgcccgg tgcaaaaata
     2101 gcaaaaaatg atgaaggcgt tagtaaagtg gttaaaatga agtgcttatt tttgcaggaa
     2161 attttacaca aggcactgag ataactatct aaagtaagta tcttgccgaa ataaatttgt
     2221 ttatggatat cacatttaat aaaaatgaag atgcattgaa gttgttggtg acaacaatga
     2281 aacaacgatt agcaaaagtt gctgaaggtg gtggaaaaaa atctattgat aaattacatg
     2341 aaaaaggaaa attacatgct cgtgagcgca tcaaattact ttttgaccag gataaacctt
     2401 gtattgaaat aggtgcattt accggatata atatgtatca ggaacatggc ggatgtccaa
     2461 gtggtggtgt ggtggtaaaa atcggatatg ttgccggtcg tcagtgtatt gttgtggcga
     2521 acgacgctac agttaaagcc ggtgcatggt ttcctatcac tggaaaaaag aatttacgtg
     2581 ctcaggaaat tgctattgaa aataaattgc ctatcattta tttggtagac agtgccggag
     2641 tatacttgcc aatgcaagat gaaatttttc cggataaaga aaattttggt cgcatattta
     2701 gaaataacgc tgtaatgagc agcatgggta ttactcaaat tgctgccatt atggggagtt
     2761 gtgttgccgg tggagcatat ttacctatta tgagcgatga agcttatatt gttaaaaata
     2821 caggaagtat ttttttagca ggtccttatt tagtaaaagc tgctattggg gaagatattg
     2881 atgccgaaac acttggcgga gcaataacta caactgaaat ttccggtatt tgcgattatg
     2941 ctgctgaaaa cgatgaggat tgtttgacac atatccgtaa aaccatggat aaaattggtg
     3001 caaaacctaa agcgggtttc gatagagttg catcaaaacc tcctgtttta aaagaagaag
     3061 atatttatgg cattatgcct gaagaaatta ccaaacaata cgatgtatta gatatcatca
     3121 aacgtttggt tgatgattct gcatttgatg aatacaaacc tgcttatggt aaaacaatag
     3181 tgtgtggatt aggtcgtatt gatggatgga gtgtgggcat tgttgccaac caacgcaaag
     3241 tggtaaaaaa taaaaaaggc gaattacaaa ttggtggggt aatttataat gacagtgcag
     3301 ataaagctgc tcgttttatt atgaattgta atcagaaaaa aattccgtta ttgtttttac
     3361 aggatgtaac cggatttatg gtaggcagta aaagtgaaca cgccggaatt attaaagatg
     3421 gcgccaaatt ggtaaatgct gtggctaatt ctgtggtgcc aaaattcaca gtaattattg
     3481 gcaatagttt cggagccggc aattatgcca tgtgcggaaa ggcatatgat ccacgattaa
     3541 ttgttgcatg gccaaatgct aaaattgcag taatgggtgg tgcgcaggcc ggtaaagtgc
     3601 tgttacaaat tcaggtggca tcgctaaaag cgaaaggcga agtaattact gccgaaaagg
     3661 aaaaagaact actagaccat attctcaacc attataatga aaccatggac ccttattatg
     3721 ctgcagctcg tttgtggata gatggcatta ttgacccgct cgataccagg aaatgggtga
     3781 gcatgggtat tgaaatggcg aataacaacg atgaaatgag agctttcaat cccggagtaa
     3841 tgcaagtgta gttggataat atgcccgtaa gtattggttt aatttagcgc tcagcaattt
     3901 atctcgagtt gtccacgtat tttcgatgct ttccatttga ggtacagcaa catcttctta
     3961 ttttcacccc atgaaaaaat ggttgggttt tgtttttatc attacaacgc tatcagcaat
     4021 agcacaaaat aaaattattc cggcaccttt gtccgtaaaa cctgcaccgg gttattttac
     4081 ttttgaccca actacctatt tacttggcaa cgaccaggta gcctataaag atggatatgt
     4141 aattaaagat tttctgataa aatattataa ccttccactt aaaactgcaa caaaaacaat
     4201 tggcgttggc aaacatatag cacttaaata tgattctaca ctcgctgtat ctgatggcgg
     4261 atatcatatt atgattagtc ctgaaaatat tactatcaca gggaaaaata atggtggtgt
     4321 tttatacgga ctgctatcgc tgatgcaatt aattgaaaaa acaggaaata cttaccaaat
     4381 accttgtgga gaaattactg attatccgca atttcaatat cgcggtatgc atctggattg
     4441 tgccaggcat ttttttgatg tagcatttat caaaaaatat atcgattatt tggccatgta
     4501 taaaatgaat acttttcatt ggcatttaac cgacgatcag
//
LOCUS       DAORKW010000277         4525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_884_length_4525_cov_4.943400, whole genome shotgun sequence.
ACCESSION   DAORKW010000277 DAORKW010000000
VERSION     DAORKW010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_884_length_4525_cov_4.943400"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /gene="kdsA"
                     /locus_tag="PLB46_10750"
     CDS             <1..604
                     /gene="kdsA"
                     /locus_tag="PLB46_10750"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:TIGR01362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRF77042.1"
                     /translation="DGMEQGLQILQQIKAEFRVPVLTDIHLPEQAAIAAGVADVLQIP
                     AFLCRQTDLLVAAGKTGIAVNVKKGQFMAPEDMAYAVEKVAGTGNNNVLLTERGASFG
                     YHNLVVDMRSLPIMRQFAPVIFDVTHSVQMPGGAGGKSGGKREFAPYLAKAAAAVGVD
                     GFFIETHPNPEVALSDGPNMVPLAEMEALLSTLMRVRQAI"
     gene            773..1225
                     /locus_tag="PLB46_10755"
     CDS             773..1225
                     /locus_tag="PLB46_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77043.1"
                     /translation="MAKEMHESTNAVRLELNKLSKAGFLVSFDEGNTIQYRANEKHPL
                     FPELKSLVRKYLGLDKVVENIVNQIGEIKFACITGDYAEGIDSGIINLVIVGEVDKDK
                     LQYWVNKTEALINRKVSTTLLNSDSFNIWKAEGNIESSIVLWTNNTTE"
     gene            1290..2168
                     /locus_tag="PLB46_10760"
     CDS             1290..2168
                     /locus_tag="PLB46_10760"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRF77044.1"
                     /translation="MFQFDYQQPKVIAEIGCNHMGDFEIAKTLIRLAKECGADYAKFQ
                     KRNPKELLSEQQYNASHPEPWQSYGETYGKHREYLEFNFEQHQQLKIYAESIGIGYSA
                     SVWDISSANDIIKLQPDFIKIPSACNLNMQLLSVLRDNYQGDVHISIGMTTAAETASI
                     VAFFNNTPENKNRLVLYSCTSGYPIKFNEICLLEINKLYQNFGDKVKAIGFSGHHLGI
                     ATDIAAYTLGARWIERHFTQDRTWKGTDHAASLEVPGLQKLVRDLHHTYEALQYKEKE
                     ILDVETVQRNKLKNNG"
     gene            2168..2896
                     /locus_tag="PLB46_10765"
     CDS             2168..2896
                     /locus_tag="PLB46_10765"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HRF77045.1"
                     /translation="MEVLAIIPARSGSKGLPGKNIKPLLQHPLIAYSIKAALSSKLIT
                     RTIVNTDSNQIAEIAKTYGAETPFLRPNELAGDFTTDLEVFQHQLQWLKDNEGYSPDL
                     IVQLRPTSPIRFDGWIDDAIIKLQNSHADSLRAITESPLTPFKMWLLNANNEEIMEPL
                     LTVKGIEEPYNQPRQKLPIVYWQTGTLDVIKTSLISENQSMSGKHILPFIIDKKYAID
                     IDDVNSFYKAEEIIQQTNCIKFDE"
     gene            2889..3854
                     /locus_tag="PLB46_10770"
     CDS             2889..3854
                     /locus_tag="PLB46_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF77046.1"
                     /translation="MNKQSPILIIGVGSIGERHINNLIQLGYTNIHVFRQRNLPLRNV
                     DSAYIQIHTDWEVVKSIPFLFAIICTPSSQHLTQTIACLDLNMHVLVEKPLSHNTNHF
                     ELLTEKVKSSGKLVYVAYMMRFHPHLIAVKNSITSNVYGKLLSIQTHWGSYLPDWHPY
                     EDYKTSYAAQKALGGGVALTLSHDIDVVNWICGATIKTVHKHFNYSPHLAIDVESVAD
                     FQIVYENDVVANVHLNYLEKTPTRIYQFLFDKAKITIDYFAATMEINNLGVITKHEIP
                     DFDRNNLFKDELTFFINTIQSDDIIHLSMNQIAESHTIINLCNHE"
     gene            3847..>4525
                     /locus_tag="PLB46_10775"
     CDS             3847..>4525
                     /locus_tag="PLB46_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF77047.1"
                     /translation="MSENISVKDKVILLTGAFGLIGKTLAKSFIAQGANVILADANKK
                     IAPQIAAEFKKLKAGKQVLVCPTDVTSLTSCNNCITLALKKFKRIDVLINNAAIDAKF
                     DKQANKVNNATRFENFPLPLLQKSFDVNVKGLVQITQLVIKQMLKQGHGNIINVASTY
                     SLVAPNQSLYNFGESQQQFKPVDYVITKSVIPNLTRYIATFYAKDNIRCNAIAPHGIF
                     NDHDDKFL"
BASE COUNT         1589 a          772 c          834 g         1330 t
ORIGIN      
        1 agatggtatg gaacaaggcc tacaaatttt acaacaaata aaagccgaat tccgagttcc
       61 tgtgttaact gatattcatt tacccgaaca agccgcaatt gcagccggag ttgcagatgt
      121 attacaaata cctgcctttt tgtgccgaca aacagatttg ttggtagcag caggcaaaac
      181 cggcatagct gtgaatgtaa aaaagggcca gtttatggca cctgaagaca tggcttatgc
      241 ggtagaaaaa gttgccggaa ccggtaataa taatgtgtta cttaccgaaa gaggcgcttc
      301 attcggatat cataatttag ttgtagatat gcgctcattg cctatcatgc gccagtttgc
      361 acctgtaatt tttgatgtta cgcatagcgt tcaaatgcct ggtggtgccg gaggaaaaag
      421 tggtggtaaa agagaattcg ctccctacct ggcaaaagca gctgcagcag taggtgttga
      481 cggctttttt atcgaaacac atcctaatcc tgaagtagca ttaagcgatg gaccaaacat
      541 ggttccactt gcggaaatgg aagcgttact ttcaaccctt atgcgtgtta ggcaggctat
      601 ttgatgttgt ctatattact acccagattt ggcgatttgc aaatgttaac caaatgagta
      661 aatttgttac tcattcagtt ttgtgttaga atccttaatc acatcgcgaa ctcgaattaa
      721 actgctgtta aagttttttt ccaacagcaa atcgtcggct catttgagag gtttggcaaa
      781 ggaaatgcat gaatctacca atgctgttcg ccttgaactc aacaaacttt ccaaagccgg
      841 tttcttagtt tccttcgacg aaggtaatac cattcaatac cgggcaaatg aaaaacaccc
      901 tctgttcccc gaattaaaaa gtttagtcag aaaatattta ggtctggaca aagtggttga
      961 gaatatcgtc aaccagattg gcgaaataaa atttgcctgt attaccggcg actatgccga
     1021 gggtatagat agtggtatta taaatctggt aattgttggt gaggtggata aggataaatt
     1081 acaatattgg gtaaataaaa ccgaagcatt aataaataga aaagtgtcga caaccctatt
     1141 aaattccgac agctttaata tttggaaagc agagggcaac atagaaagtt caattgtatt
     1201 gtggactaac aatacaactg aataattaat ttattattta actgtctgct caacacacta
     1261 tgtgactgag gaggcgaagc tgtaaaaaga tgtttcaatt tgattatcaa caaccaaagg
     1321 tgattgcaga aattggatgt aatcatatgg gtgattttga gattgcaaaa acgctaatta
     1381 ggttagcaaa agaatgtgga gccgattatg caaaatttca aaaacgcaat cccaaagaat
     1441 tattaagcga acaacaatac aatgcttcac accctgaacc atggcaaagt tatggtgaaa
     1501 catacggcaa acatcgcgag tatctggaat tcaattttga acaacatcaa caattaaaaa
     1561 tatatgctga atctattggc attggatata gtgcatcagt ttgggatata agtagtgcta
     1621 acgatatcat taagttacaa cctgacttta ttaaaattcc ttctgcctgt aatttaaata
     1681 tgcagttatt gagtgtttta cgagataatt atcagggaga tgtgcacata tcaataggta
     1741 tgaccaccgc agcggaaaca gcatcaatag tagcattttt taacaatacg cctgaaaata
     1801 aaaacagact agtattatac agttgcacga gtggttatcc aataaaattt aatgaaatct
     1861 gtttgctaga aataaataaa ttatatcaaa attttggcga taaagtgaaa gcaattggat
     1921 ttagtggcca tcatttaggt atagcaactg atattgctgc ttacacactt ggtgcccggt
     1981 ggattgaacg tcattttaca caagacagaa cctggaaggg cacggatcat gctgcaagtt
     2041 tggaggttcc cggcttacaa aagttagttc gcgatttaca tcacacttac gaagcattac
     2101 aatataaaga aaaggaaata ttagatgttg aaactgttca acgcaacaaa ttaaaaaata
     2161 acgggtaatg gaagttttag caataattcc ggcaaggtcg ggtagtaaag gtttaccggg
     2221 caaaaatatt aagccattgt tgcagcatcc attaattgcg tatagtatta aggctgcatt
     2281 atcaagtaaa ttaattacca gaacaatagt gaatactgat tcaaatcaaa tagctgaaat
     2341 agccaaaaca tatggtgcag aaacgccatt tttaagacca aatgaattag ccggtgattt
     2401 tactactgat ttagaagtat ttcaacatca attgcaatgg ttaaaagata atgaaggata
     2461 ttctcccgat ttaatagtgc aacttcgtcc cacttcgcca attcgttttg atggttggat
     2521 agatgatgct attatcaaat tgcaaaacag tcatgcagat tcattacgtg caataacaga
     2581 gtcgccatta acaccattta aaatgtggtt actcaatgcc aacaatgaag aaataatgga
     2641 gccattgtta acagtaaagg gcatagagga accatataat cagcccaggc aaaaacttcc
     2701 gattgtatat tggcaaacag gaactttaga tgttattaaa acatcactta tcagcgaaaa
     2761 tcaatccatg agtggtaaac atattcttcc attcattata gataaaaaat atgccatcga
     2821 tattgacgat gtaaatagtt tttacaaggc ggaagaaatt atacagcaaa ccaattgtat
     2881 aaagtttgat gaataaacaa tctcccatat taataatcgg tgtaggttca attggagaaa
     2941 ggcatatcaa caacctcatt cagttgggat atacaaatat tcatgttttt cgccagcgta
     3001 atttaccttt aagaaatgta gattcagcat atattcagat acatacagat tgggaggttg
     3061 ttaaaagcat tccattttta tttgccataa tttgcacacc cagcagtcag cacttaacac
     3121 aaacaatagc atgtctcgat ttaaacatgc atgtattggt tgaaaaacca ctttcacata
     3181 atactaatca ttttgagtta ttaactgaaa aggtaaaatc ttcaggcaaa ttagtttatg
     3241 tggcatatat gatgcgattt catccacatc tcattgcagt aaaaaatagt attacctcaa
     3301 atgtgtatgg caaattatta tccattcaaa cacattgggg cagttattta cccgactggc
     3361 atccttatga agattacaaa acaagttatg cagcacaaaa agcacttggt ggtggtgttg
     3421 ccttaaccct gagtcatgat atcgatgtag tcaactggat ttgcggagct accattaaaa
     3481 ccgttcacaa acattttaat tattcaccac atttggctat agatgtcgaa agtgtagccg
     3541 attttcaaat tgtttatgaa aatgatgttg tagctaatgt gcatttgaat tatttagaaa
     3601 aaacaccaac acgtatttat cagtttttat ttgataaagc caaaatcacc attgactatt
     3661 ttgcagcaac catggaaata aacaatttgg gtgttatcac taaacatgaa atacccgact
     3721 ttgaccgtaa caacctattt aaagatgagt taactttttt tataaacaca atacaatcag
     3781 acgacataat acatctttct atgaatcaga tagcagaatc ccacactata ataaatttat
     3841 gcaaccatga gtgaaaatat ttccgttaag gataaggtaa ttttattaac cggtgctttt
     3901 ggtttaatag gcaaaacctt agcaaaaagt tttatagcac aaggagcaaa tgtgatttta
     3961 gcggatgcca ataaaaaaat agcaccccaa atagcagctg aatttaaaaa attaaaagcc
     4021 ggcaagcagg tattagtttg cccgaccgat gtaacaagtc ttacatcatg caataattgt
     4081 atcacattgg cccttaaaaa atttaaacgt attgatgttt taataaataa tgcagctatt
     4141 gatgcaaaat ttgataaaca ggcaaacaaa gtaaacaatg ctactcgctt tgaaaatttt
     4201 ccattaccgc ttttgcaaaa gtcgtttgat gtaaatgtga agggtttagt tcaaatcacc
     4261 cagctggtaa tcaaacaaat gttaaaacaa ggtcatggca atatcattaa tgtagcttct
     4321 acttattcat tagttgcacc taaccaatcg ttatacaact ttggtgaaag tcagcaacaa
     4381 tttaagccgg tagattatgt aattaccaaa tcggttatac caaatttaac aagatatatt
     4441 gccacatttt atgcaaagga taatatcaga tgcaatgcaa ttgcaccgca tggtatattt
     4501 aatgaccatg atgataaatt tttgc
//
LOCUS       DAORKW010000278         4513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_888_length_4513_cov_4.196052, whole genome shotgun sequence.
ACCESSION   DAORKW010000278 DAORKW010000000
VERSION     DAORKW010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_888_length_4513_cov_4.196052"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLB46_10780"
     CDS             <1..749
                     /locus_tag="PLB46_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77048.1"
                     /translation="IELSDETGAFADPVTIGSVTTTALTGNISCTIPATAVLGSDYRM
                     RATSTQQEYIGLKNGSDLSIVCSKPINVETATINPTSVTLTWDASLCGVAYEVEYKQV
                     AAGTWTVVTTPTNSITITDLVPNTAYEWAVRTICVESPLVQSTYTSIEDFVTGQNSVQ
                     IIPGLSHIAIFPNPVSTTASVEIVLTQTTELSIELLDILGQSISVLVDDKLNAGSHTF
                     ELNRNQLNSGVYTLQITSGKNVVAKNIVIE"
     gene            complement(801..2465)
                     /gene="recN"
                     /locus_tag="PLB46_10785"
     CDS             complement(801..2465)
                     /gene="recN"
                     /locus_tag="PLB46_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRF77049.1"
                     /translation="MLKELRIRNYAIINEASISFSGGMNIITGETGAGKSILLGALSL
                     ILGERAESKVLFNKDKKCVVEGVFDISNYQLADFFAANEIDFENETIIRRELTESGKS
                     RAFINDTPVNLNVIQQLAGRLITIHSQHETLDLGNSGFQLTVVDALAETSMLVSTFKT
                     HYQAWKTCAKKLSETEALAAKALTEKDFVEFQFTELDNAALDGIDQLQLETELTQLSH
                     AEEIKRNLMLSVDGLENGQGNINDQLRAVINQLNTISKYLPELADYIQRLESANIELR
                     DISRDLDDKAAATIADPARSEIIQTQLNSIYRLQKKHGCNTLSDLIVLRDGLGEKLLA
                     YVNSEAAIETLKKERDALFVKLSLAAKKLSEKRAGAFEQLEKSVHLLLAEVGMKDAVL
                     KVEHQFSTDKYLTETGADHIQFMFSANKGSSLQDIKKVASGGELSRLMLCIKSLLAKS
                     VALPTLIFDEIDTGVSGEIAHKVGNILMRLSNNHQLIAITHLPQIAAKGQHHLFVYKS
                     TDAASTSTNIKLLNKTERLNEIAKMLSGENPTKAAIQNAKELLLQA"
     gene            complement(3300..4199)
                     /locus_tag="PLB46_10790"
     CDS             complement(3300..4199)
                     /locus_tag="PLB46_10790"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HRF77050.1"
                     /translation="MRNIILLLLLTGFGSSMFAQDLNCVVTVNAEKVTQTDPKIFKTL
                     ETSIFEFMNTRKWSDDVFKPEERIECQILITITEELSSDKFKAQASIVSRRPVYGSDY
                     NSTLLNFVDKDFEFTYAEYQPLEYNDNQYTSNLTAMLAYYAYMIIGLDYDSFAQKGGD
                     KYFLKAQTIVNQASNSDSKGWKSFDGTRNRYWYVTNILDPKFAGIRDAIYQYHRQGLD
                     QMYNDQTKPITIITKALQTLDNTNRTSPNSMFVQLFFSAKSDELLGLYSKATPAEKSK
                     AVSYLTRLDPVNADNYQELLTGK"
     gene            complement(4192..>4513)
                     /locus_tag="PLB46_10795"
     CDS             complement(4192..>4513)
                     /locus_tag="PLB46_10795"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRF77051.1"
                     /translation="DETLNISLKRNPDILKTLSSQRNKQFIVGFALETDNEFANAAKK
                     LREKGLDMIILNSMNDTGAGFQHDTNKISILDKYDNQINFDLKSKKAVAFDIVNHIIN
                     KLNA"
BASE COUNT         1315 a          864 c          836 g         1498 t
ORIGIN      
        1 caattgaatt gagcgatgaa actggtgcat ttgccgaccc ggttacaatt ggtagtgtaa
       61 ctacaactgc cttaaccgga aatattagtt gtacaattcc tgctactgca gttttgggct
      121 ctgattacag aatgcgtgca acaagtactc aacaagaata tatcggatta aaaaatggca
      181 gcgatttatc tatcgtttgc agtaaaccta tcaatgtaga aacagctaca attaatccta
      241 ccagtgtaac acttacttgg gatgcttcac tttgtggtgt agcctacgaa gtggagtata
      301 aacaagttgc tgctggtaca tggactgtag taactacgcc tactaacagc atcacaatta
      361 cagatttggt tccaaataca gcatacgaat gggcagttag gactatttgc gttgaatcac
      421 ctttagttca atctacttat accagtattg aggattttgt tacgggacaa aatagtgttc
      481 aaataattcc gggtttatcg catattgcta ttttcccgaa tcctgtatca acaactgctt
      541 cagtagaaat tgtgcttaca caaactactg agttaagcat tgaattactc gacattttag
      601 gtcaaagcat ttcggtatta gtggatgata aactcaatgc aggttcacat acttttgaat
      661 taaaccgcaa tcaactaaac agtggtgttt atacattgca aattaccagc ggtaaaaatg
      721 tagttgcgaa aaatattgtt attgaataaa agtatctatt tgtgcaaaac gggaagtgcc
      781 atcggtattt cccgtttttt ttatgcttgt aaaagcaact cttttgcgtt ctgaatagca
      841 gcttttgtcg gattttctcc gctcagcatt ttagctattt catttaatct ttctgtttta
      901 ttcagcagtt taatattggt agaagtactt gctgcatcgg tcgatttata cacaaataaa
      961 tgatgttgcc ctttggctgc aatttgcggt aaatgggtaa tcgctatgag ctgatgattg
     1021 ttagaaagac gcattaatat attgccaact ttatgtgcaa tttcacctga aacaccggtg
     1081 tctatttcat caaaaattaa agtgggtaat gctacacttt ttgccagtaa cgatttaatg
     1141 cataacatta aacgcgataa ctctccacca ctggccactt ttttaatatc ttgtaaactg
     1201 cttcccttgt tggctgaaaa cataaattgg atatggtcgg caccggtttc agttaaatat
     1261 ttatcggtgc tgaactgatg ttctactttt aaaacggcat ctttcatacc tacttcggct
     1321 aataaaagat gtaccgattt ttccaattgc tcaaatgcac cagctctttt ttcagataat
     1381 ttttttgccg ctaatgataa cttcacaaat aaagcatcac gctctttttt caaagtttca
     1441 atagcagctt ctgaatttac gtaggctaat aatttttcgc ctaaaccatc ccgcaaaaca
     1501 attaaatccg aaagcgtatt acaaccatgt tttttttgta atcgataaat actattgagt
     1561 tgtgtttgta taatttcact tcttgcaggg tcagctatag tcgcagcagc tttatcatcc
     1621 aaatcacggc taatatctct caattcaata ttcgctgatt ctaaacgttg gatataatcc
     1681 gctaattccg gcagatattt actaatggta ttgagctgat ttattaccgc tcgtaactgg
     1741 tcattaatat tcccttgtcc attttccaag ccatcaacac tcaacattaa attgcgttta
     1801 atttcctccg catgtgaaag ttgagtaagt tctgtttcta attgtaattg gtcaatacca
     1861 tctaaagcag cattgtcaag ttcggtaaat tgaaattcca caaaatcttt ttcggtaagt
     1921 gctttcgctg ccaatgcctc tgtttcggat aatttttttg cgcatgtttt ccatgcctga
     1981 taatgtgttt taaatgttga aacgagcata gatgtttcag cgagcgcgtc cacaaccgta
     2041 agttgaaatc cggaatttcc taaatccagt gtttcatgtt gcgaatggat agtaattaag
     2101 cgaccagcca actgttgaat tacatttaaa ttcacaggag tatcattgat aaacgcacgt
     2161 gattttccac tttcggtaag ttcacggcga atgatggttt cattttcgaa gtcaatttca
     2221 ttcgctgcaa aaaagtccgc caactgataa ttactgatgt caaaaacccc ttcaacaaca
     2281 cattttttat ccttattgaa taataccttg ctttctgcgc gttcacctaa aattaaactc
     2341 agggctccca ataaaatcga tttccctgct cctgtctcac ccgtaatgat attcattcct
     2401 ccggaaaaag aaatggaggc ttcattaatg atggcataat tccgaatacg cagctccttc
     2461 agcatgccgt aaagatataa gttgaatagg agatatggta gtttcacgcg gagttggttt
     2521 ctcgcaaagg cccaaagatt taatatttat atgatgtatg atgtagtatg tttttaagcg
     2581 cgcggagagg gttgctcgca aaggcccgaa gtggaaatgt ttatggatgg tgcgatatgg
     2641 attgttttta agcgcacaaa ggctttaaaa acattattat cgaaaaatga gtaatttttt
     2701 gttgtagggg cgaattgcat ccgtccttac aataaaaaat tgtggcttga tttgcataaa
     2761 tgaacagggt tcagcaatgc cacctttgcg tgcttgaatt ggttttttat ttataaaaaa
     2821 tacatcatat ggaactcaat taactttggg cctttgcgag aaacatttcg aaaattatat
     2881 gatgtgtttg tttatgaggg cacggagctg gtttctcgca aaggcccaaa gtttattggt
     2941 ttatattttg tgtgatatgg attgttttca agcgcgcaaa gctgttaaac caataccacc
     3001 tttgcgtgct tgaattggtt taataaacga accaagtaca ttccactaaa accctcactt
     3061 agcaccttgg cgagaaacat tccgaaaatt gaatgatgtg tttttttatg agggcacgga
     3121 gctggtttct cgcaaaggcc caaagtttat tggtttatat tttgtgtgat atggattgtt
     3181 ttcaagcgcg caaagctgtt aaaccaatat caccctttgc gtgcttgaat tggtttatta
     3241 tttgaaccaa gtacattcca ctaaaaccct cacttagcgc ctttgcgaga aacaccacct
     3301 tacttccctg tcagcaattc ctgataatta tccgcattta cgggatcaag tcgagtcaag
     3361 taagagactg ccttcgattt ttcagccggg gtagcttttg aatacaatcc taataattca
     3421 tcactcttag cactgaaaaa caactgcacg aacatactgt ttggagaggt gcggttagtg
     3481 ttatctaagg tttgcagagc cttggttata attgttatcg gtttggtctg gtcgttatac
     3541 atttggtcca atccctgacg atgatactga taaatcgcat cgcgtattcc tgcaaatttt
     3601 gggtcgagaa tattagttac ataccaatat cggttacggg taccatcaaa actcttccag
     3661 cctttgctgt cgctgttgct tgcctgattg acaattgtct gggcctttaa aaagtatttg
     3721 tcgccacctt tttgagcaaa tgaatcgtaa tctaacccaa taatcatata cgcataataa
     3781 gccaacatcg ctgtcaggtt agaggtatac tgattatcat tatattccaa tggctggtat
     3841 tcagcataag tgaattcaaa gtctttatct acaaaattta gtaaggtaga attgtaatcg
     3901 ctgccataca ccggacgacg ggatacaata ctggcctgtg ccttaaactt atcgctggaa
     3961 agttcttctg taatagttat aagtatctga cattcaatgc gttcttcagg cttaaataca
     4021 tcatcactcc acttacgagt attcataaat tcgaagatgg aagtctccaa agttttaaaa
     4081 atcttagggt ctgtttgggt aactttttca gcatttacag taaccacaca gttaagatcc
     4141 tgtgcaaaca tggatgatcc aaagccagtt aagaggagta gtaaaatgat attacgcatt
     4201 cagcttattt attatatggt tcacaatatc gaaggcaaca gcctttttgc tcttcaaatc
     4261 aaagttaatt tgattatcgt atttatccaa tatcgaaatc ttattggtat cgtgttgaaa
     4321 tccggcaccg gtatcgttca tcgaattaag gataatcata tctaaccctt tctcccgcaa
     4381 tttctttgcc gcatttgcaa attcattgtc ggtttctaac gcaaatccta caataaactg
     4441 tttatttcgc tgcgaactca gggtttttaa tatatcggga ttgcgtttca ggctgatgtt
     4501 aagggtttca tcg
//
LOCUS       DAORKW010000279         4510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_892_length_4510_cov_4.683277, whole genome shotgun sequence.
ACCESSION   DAORKW010000279 DAORKW010000000
VERSION     DAORKW010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_4510_cov_4.683277"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..965)
                     /locus_tag="PLB46_10800"
     CDS             complement(66..965)
                     /locus_tag="PLB46_10800"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRF77052.1"
                     /translation="MKIVKLLGFIFLSGSLSAQMSYFEIGLMGGVANYYGDMTHDYVV
                     LKESHPAYGGFVKYNVDAKKGFKFNVYSGTISAADKNSDRANLNARNLSFTSNVTEVA
                     FTFEYNFLGYRPVEFKQRISPYAYAGLAGFHFNPQAYYEGEWYDLQPLGTEGQGMENF
                     PYRDKYRLYEFAFPFGVGIKFAMTERWNLGLEYGARWTFTDYLDDVSRTYVDRNLLIE
                     ANGIDAYNLSNRSGEYLLGEPFDYQNNDWRGDPTNTDWYMWFGLTLSRNMIKGVEEGF
                     RTSTKDILGCPGTRHINKPKKRR"
     gene            complement(1109..2026)
                     /locus_tag="PLB46_10805"
     CDS             complement(1109..2026)
                     /locus_tag="PLB46_10805"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HRF77053.1"
                     /translation="MKRIVLILCLGLTLSHAHAQQYVEVGVFGGLSNYQGDLAPVAFV
                     PKETHAAFGAFYKFNLNYWFAFKGSVYSGLISGNDDNSTEEWKQQRNLSFQTPIQEAS
                     LYFELFLKKYNPNYKWNIMSPYIFFGISLYHFNPRANYMGVWYDLQPLGTEGQGNPAY
                     PDRVPYKLTQYAYPMGFGLKGSVTKYWNIGLEMGYRKTTTDYLDDVSTTYIAKELLLA
                     DNGDIAWQLSNRSDEMNGGIEILRDDKKYRGDDTNKDWYIFTGLTISRNIEVKGWESK
                     KYKASDNPKKPGLKKSRKKMSCPGINQKL"
     gene            2205..2918
                     /locus_tag="PLB46_10810"
     CDS             2205..2918
                     /locus_tag="PLB46_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HRF77054.1"
                     /translation="MKSKKISLLLSLLGASAGADRFYLGQNTAGWVTLLTFWVLIPGA
                     VFGIIKFNLFPNWEPFMLGRFALPIVFHLYETGRYMVISDEKFMSQDVSKSKTFPLTI
                     ASFIIAALLIAGGSRLLKSAMVVNIETAEISAVLSAETMSQEFRNNEDLYRKKYDNLV
                     LEIEGIVSETGNDFETGTYFALKGLNNDPFGIKCYFLEHNIPDANKVTIGDKVIMKGV
                     ANGNKLENCKVISINGKTL"
     gene            2940..3569
                     /gene="recR"
                     /locus_tag="PLB46_10815"
     CDS             2940..3569
                     /gene="recR"
                     /locus_tag="PLB46_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRF77055.1"
                     /translation="MNLPSQWMEQAVNAFAKLPGIGKKTALRLVLHLLKQKKEDVEVF
                     ASAMLKLKTDIHFCPNCNNISDLAGTLCTICSNNKRDHKIICVIESLRDLIAIESTNQ
                     FFGEYHILGGVISPMEGIGPEQLFLEKLIEKANSGNYNEIIMALNPNIEGDTTIFYIT
                     KKITNKQIKITTIARGISFGGELEYTDELTLARSLATRRPFENYMQQNE"
     gene            3566..>4510
                     /locus_tag="PLB46_10820"
     CDS             3566..>4510
                     /locus_tag="PLB46_10820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF77056.1"
                     /translation="MIQLSVIIVNYNVKYFLEQAVLSALKACENIAAEIIIVDNHSVD
                     GSVEFITQKFIDSGKSTTPIHIIANKENTGFSKANNQGITIANGKYVLLLNPDTVVEE
                     DTFEKCLAFMDARPDAGGLGVKMIDGKGNFLPESKRAFPSPEVAFYKAFGLANIFPKS
                     KIFGKYHLGYLDENTTHEVDVLAGAFMLIRKTVLDKIGYLDEAFFMYGEDIDLSYRIV
                     QAGYKNYYFADTRIIHYKGESTKKASFNYVKMFYNAMKIFANKHFTGRRAGIFVSLLN
                     IAIYFRALIATTVRILQKIALPFLDAIIILGGMYLVKEY"
BASE COUNT         1442 a          830 c          849 g         1389 t
ORIGIN      
        1 ggttatacac ataaaaaaac gggagttcaa actgaagctc ccgttaatat ttttaatagc
       61 tactattatc ttcttttttt cggtttatta atatgacgtg ttcccgggca acctaaaata
      121 tctttggtag aagttctgaa accttcttcc actcctttaa tcatatttct gctaagtgtt
      181 aaaccaaacc acatatacca gtcggtatta gtagggtcac ctctccaatc gttgttttgg
      241 tagtcgaatg gttcacccaa taaatattca ccgcttcggt tagacaggtt ataagcatca
      301 ataccgtttg cttcaataag caaattgcgg tctacatagg tgcgactaac atcatccaga
      361 tagtcggtaa aggtccatct tgctccgtat tctaacccaa ggttccaacg ttcagtcatt
      421 gcaaatttaa ttcctactcc aaaaggaaag gcaaactcat aaaggcgata tttatctctg
      481 taaggaaaat tctccattcc ctgcccttcg gtacctagtg gctgtaaatc gtaccattca
      541 ccttcataat aagcttgtgg attaaaatga aacccagcca aacctgcata agcataagga
      601 gaaatacgtt gtttaaattc aacaggacga tatcctaaaa aattatattc aaaagtaaat
      661 gccacttcag ttacattaga agtaaaactt aagtttctgg cattaagatt agctctgtcg
      721 ctatttttat cggcagctga aatagttccg ctataaacat tgaatttgaa gccttttttg
      781 gcatcaacat tatattttac gaatccgcca taagcaggat gtgattcttt aagcaccaca
      841 taatcgtggg tcatatcgcc gtagtaatta gccacgcctc ccatcaagcc tatttcaaaa
      901 taggacatct gggccgataa tgaaccagaa aggaatataa aaccgaggag tttgacaatc
      961 ttcatccgat acttggtttg tttaattaaa acaaaaataa ggttttgttc gataaactgg
     1021 taaaattgct tgtttattat aactcaaaaa tgacattttt attgtgtccg gtgtcgaaaa
     1081 tattcaacag tgtgtggttc tggaggggtt ataatttttg atttatgccc ggacaagaca
     1141 tttttttacg tgatttttta aggcccggtt tcttagggtt atcgctggct ttgtattttt
     1201 tagattccca gcctttcact tcaatgttgc gtgaaatagt taacccggta aagatatacc
     1261 agtccttatt ggtatcatcg cctctgtact ttttatcatc tcttaaaatt tcaataccgc
     1321 cattcatttc atctgacctg ttagacaatt gccatgcgat atcaccattg tcggccaaca
     1381 gcaactcttt ggcaatatag gttgtgctaa catcatcgag gtagtcggta gtggtttttc
     1441 gataccccat ttccaaacca atattccaat atttggttac ggagcctttt aacccgaaac
     1501 ccattggata agcatattga gtaagtttat acggaactct gtcgggatat gccggattgc
     1561 cctgaccttc ggtaccaagt ggctgaaggt cgtaccaaac gcccatataa ttggctcttg
     1621 gattaaaatg gtaaagtgaa ataccaaaga aaatataagg ggacatgata ttccacttat
     1681 agttgggatt gtattttttc aagaatagtt caaaatagag tgaagcttcc tgtatagggg
     1741 tttggaagct aaggttacgc tgctgtttcc attcttccgt tgagttgtca tcattaccgg
     1801 aaatcaaacc gctgtaaaca cttcctttaa aggcaaacca ataatttaag ttgaacttat
     1861 aaaaagcgcc aaatgcagcg tgagtttcct ttggcacaaa tgcaacaggg gctaaatcgc
     1921 cctggtagtt gctgagaccg ccaaaaacgc ctacctcaac atactgttgt gcatgggcat
     1981 gacttaacgt caaccccaaa cataggatta gtactattct tttcatcaac ttgagtggta
     2041 tcatggatta ctgttgctct tacaaatatg ctgaactctg tacaataatc gttgacaccg
     2101 ggggcaaaaa tagccatttt aactaaaacc aaaaatgtca catttttcgg cattgcagcg
     2161 aaatcgtata aatgtgtcta tttttgcgga tagctgttaa aactatgaaa tcaaaaaaga
     2221 ttagcttatt actgtcgttg ttaggcgcaa gtgccggggc cgacagattt tatttagggc
     2281 agaatacagc agggtgggtt actctcctca ctttttgggt acttatacct ggggcagtat
     2341 ttggcatcat taaatttaat ctgttcccca attgggagcc ctttatgctt ggacgctttg
     2401 ccctgcctat tgtgtttcat ttgtacgaaa caggccgata catggttata agtgatgaaa
     2461 aattcatgtc acaagatgta tcaaaaagta aaacttttcc tttaactatt gcatctttta
     2521 ttatagccgc tcttttaatt gcaggtggca gcaggctgct aaaatcggca atggtagtta
     2581 acattgaaac agcagaaatc agcgcagtcc tctctgccga aactatgtca caggaattta
     2641 gaaataacga agacctttac agaaaaaaat acgataacct tgtcctagaa attgaaggta
     2701 tcgtaagcga aaccggcaac gactttgaaa ccggaactta ttttgcgcta aaaggcctaa
     2761 ataatgatcc ttttggcata aaatgttatt ttttggagca caatatacca gatgccaata
     2821 aagttactat tggcgacaaa gtaattatga aaggtgtggc caatggtaac aaactggaaa
     2881 attgtaaagt aataagtatt aacgggaaaa cattataatc accatttgaa acaactcgcg
     2941 tgaatttacc ttctcaatgg atggaacaag ctgtaaatgc atttgccaaa ctaccgggta
     3001 ttggcaaaaa aacggcatta cgccttgttt tacatttact gaaacagaaa aaagaagatg
     3061 tagaagtatt tgcttcggcg atgttaaaac tcaaaacgga tatacatttt tgtcctaatt
     3121 gcaataatat ttccgattta gccggcacat tatgcaccat ttgcagcaac aacaaacgcg
     3181 accataagat aatttgtgtt attgaaagtt tgcgcgattt aattgcaatt gaaagtacca
     3241 atcagttttt tggcgaatat catattttag gtggtgtaat ttcgcctatg gaaggcattg
     3301 gtcctgaaca attatttttg gaaaaactta ttgaaaaagc caactcaggt aattataatg
     3361 aaataattat ggccttgaat ccaaatattg aaggcgatac gaccattttt tatatcacca
     3421 aaaaaataac caataaacaa atcaaaataa ccaccattgc cagaggcatt tcttttggag
     3481 gtgaattgga atataccgac gaacttacct tagcacgctc gcttgcaaca cgacgcccat
     3541 ttgaaaacta tatgcaacaa aacgaatgat tcagttatcc gtaataatag ttaattataa
     3601 tgtcaagtat tttcttgagc aagctgtttt atcagcactt aaggcttgtg aaaatattgc
     3661 tgcggaaatt attattgttg ataatcattc tgttgatggt agtgtggaat tcattacaca
     3721 aaagtttatt gatagcggaa aatcaacaac accgatacat attattgcca ataaagaaaa
     3781 caccggattt tcgaaagcaa ataatcaggg gataaccatt gcaaatggta agtatgtttt
     3841 attgcttaat ccggacacgg tggttgaaga agatacattt gaaaaatgtt tagcctttat
     3901 ggatgcccga cctgatgcag gtgggctcgg agtgaaaatg attgatggaa aaggcaattt
     3961 tttacctgaa agcaaacgcg ctttccccag tcctgaagtg gcattctaca aggcttttgg
     4021 cttggcaaat atatttccga agtcgaaaat attcgggaaa taccacttag gttatctgga
     4081 tgaaaataca acgcatgaag tggacgtttt agcgggcgca tttatgctta tacgcaaaac
     4141 agtattagat aaaatcggat atttggacga ggcatttttt atgtatggcg aagacatcga
     4201 tttaagttat cgcattgtac aggcaggata taaaaattac tatttcgccg atacacgaat
     4261 aatccattac aaaggtgaga gtaccaaaaa agcgagtttt aattatgtga aaatgtttta
     4321 taatgcaatg aaaatatttg ccaataaaca ttttacagga aggcgagcag gcatatttgt
     4381 ttcactgctc aacatcgcta tttatttcag agctttaatt gctaccactg tcaggatatt
     4441 acaaaaaatt gcacttccat ttttagatgc tattatcatt ttaggtggca tgtatttagt
     4501 gaaggaatat
//
LOCUS       DAORKW010000280         4508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_895_length_4508_cov_4.749158, whole genome shotgun sequence.
ACCESSION   DAORKW010000280 DAORKW010000000
VERSION     DAORKW010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_895_length_4508_cov_4.749158"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PLB46_10825"
     CDS             complement(<1..843)
                     /locus_tag="PLB46_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRF77057.1"
                     /translation="MDSGEKQPLLQKVFSAITKPLSPYYQKYIGKYIGEIASVYPYPV
                     KVLRISLRSMAAVAGCLFVLYLLILVGAFGIVPGKSELKAVNNYTASEVYSQDSILLG
                     KYFIQNRTNAEFEDLPPHLINALVSTEDSRFYEHKGVDFVSFMRVLIMSIILNDDAGG
                     GSTITQQLAKNLYMRKDYGALSMPVNKFREMIIAGRLEDVYEKEQILTLYFNTVPFGE
                     DCYGIEAGSLRFFNCAPKDLTIEQSAVLVGLLKANTTYNPRLNPDASLIRRNTVLNLM
                     VQHEN"
     gene            complement(934..1413)
                     /locus_tag="PLB46_10830"
     CDS             complement(934..1413)
                     /locus_tag="PLB46_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77058.1"
                     /translation="MKNIKLTIMILIALIKRYNKTISIITELVEAITTIVDTMKKNAI
                     IKLINVVIGMAIGKVIENYLDELIVTIIDIIKILYKYEIYMYIINSQLAAIIIAILTF
                     QTALIINEITIIITIGLVVTIAITVVLSELFLITKNSSANHPKSICSRVGYGGLLPE"
     gene            complement(1729..2613)
                     /locus_tag="PLB46_10835"
     CDS             complement(1729..2613)
                     /locus_tag="PLB46_10835"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HRF77059.1"
                     /translation="MYRTFTFFVLMAISGLLSAQNTVYATETFRDTRIISGQSVETSI
                     QGQAKFIISHRFGDIYEQNPFSLLYNFFGFNGGANMRIGLDYGITNWLTVGAGRSNLA
                     RTYDGFVKVKLLKQSTGDKNMPITLTLYSDISVITDTSDELLDPYFTGGIGFANQLLI
                     ARKFSDGFSLQLMPTHVHRNLVDLNSDPNDIFAVGIATKVRVAKNIDLTGEYFYTLPD
                     QLPVGYDNCLSIGADFITKGHVFQLQFSNSPYLIPEHYIGKTQGSIYETVNGKPEFNI
                     RFGFNITRTFKVAGRPYE"
     gene            complement(2624..3019)
                     /locus_tag="PLB46_10840"
     CDS             complement(2624..3019)
                     /locus_tag="PLB46_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77060.1"
                     /translation="MKKTPLIAAGMLCIAFFFNSCYYDSKEELFGTTACDTVDVTYAT
                     TILPILETSCLECHSEANAGVLGGSQILEGYDFLMDYVVAGDANASSFYNTVAWVSGY
                     VEMPQGSDQLSDCKIAKIRAWINAGALNN"
     gene            complement(3021..3716)
                     /locus_tag="PLB46_10845"
     CDS             complement(3021..3716)
                     /locus_tag="PLB46_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77061.1"
                     /translation="MKTAKFVGIVALSIASIVAGCKHNTLIDLTNPTVTVDCDPDTVY
                     FQNDILPLLISNCAMSGCHDGIGRVDEKAPLGSYEDVMNSGYVKAFDANDSKLIEAVT
                     DGGEDFMPPSPNEPLSNAQIALLEKWINQGARNNACEGGDCDTTAVTYSGKIVKIVDT
                     YCLGCHNSTTTSSGIDLSSYSGVADIAADGSFLGTIMFEAGYVGMPYNGNPLPDCKID
                     EIRIWIENGYLNN"
     gene            complement(3805..>4508)
                     /locus_tag="PLB46_10850"
     CDS             complement(3805..>4508)
                     /locus_tag="PLB46_10850"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77062.1"
                     /translation="AASIQSFKHSPLDILIEDPIEPIDTTGGGDTTLITSNCDPDTVY
                     FENDIYPILISNCAISGCHDAETHEEGINLSTYAKVISSHIMKPSDPYDSEFYDVVTE
                     TGDDLMPPPPMASLTDEEIALIILWQEQGALDNSCTDCDTSAVTFSGTILPIMNAYCT
                     GCHDASSPSGGISLTAYLGTGSNDGIVDVASDGRLMGALNGDVGFVAMPPGSMLPQCL
                     IDQIRIWVDAGYPED"
BASE COUNT         1400 a          871 c          784 g         1453 t
ORIGIN      
        1 attttcatgt tgcaccatga ggtttaaaac ggtatttctc ctgattaatg aggcatccgg
       61 atttaaacgc ggattataag tagtattggc ttttaaaagg ccgactaaaa cagcactttg
      121 ttctatggtg aggtcctttg gtgcgcaatt aaaaaaacgt aaggatccgg cttcaattcc
      181 ataacaatct tccccgaaag gcacggtatt aaaatacaag gttagtattt gttctttttc
      241 gtaaacgtct tccaggcgac cggcaataat catctcccta aatttattta ccggcattga
      301 caaagcgccg taatccttcc gcatataaag gtttttggcc aattgttggg tgatggtgct
      361 tccgccacct gcatcatcat tcaatatgat agacataatg agtacccgca taaaactcac
      421 aaaatctacg cccttatgct cataaaaacg actatcctcg gtggaaacca gtgcattgat
      481 aagatgtgga ggtaaatcct caaactctgc attggtgcgg ttttgaatga aatatttacc
      541 taataaaata ctatcctgac tatacacttc tgaagccgta taattattta cagctttcag
      601 ctccgattta ccggggacta taccgaatgc acctactaaa atcaataaat acaacacgaa
      661 taaacacccg gcaactgctg ccattgatcg caatgaaatg cgtaatactt taacagggta
      721 ggggtatacc gaggcgattt cgccaatgta tttccctata tatttttggt aataagggct
      781 caaaggcttg gtaatagcag aaaatacttt ttgtaataat ggttgttttt cgcctgaatc
      841 catatgcaaa tgtatgattg aaaattgttt ttttaagcat aaaaaggccg ggaactttta
      901 tcgttggaca tcagttcacg gcctctggct gatttattcg ggtagtaaac cgccataccc
      961 gactcgggag cagatacttt ttgggtgatt ggcactacta ttttttgtaa ttagaaatag
     1021 ttcactcaac accacagtta ttgcaattgt tacaactaaa ccaattgtta taatgatagt
     1081 aatttcgttg attattaatg ctgtttgaaa agtcaaaata gcaataataa ttgcagctaa
     1141 ttgactattg attatataca tataaatttc atacttgtat aaaattttaa tgatatcaat
     1201 tatagttaca attaattcgt ctaaataatt ttcaataact tttccaatag ccataccaat
     1261 taccacatta attaatttaa tgatggcatt ttttttcatg gtatcaacaa ttgtagtaat
     1321 tgcctccact aattcagtaa tgatactaat tgttttattg taccttttaa ttaaggcaat
     1381 taatatcatg atggttaatt taatattttt cataaaaatt atttcagctt tgaggtaaat
     1441 aatgaattta ccgggcataa aaatgcccgg aatgcaccaa acatccgggc tgtaaataat
     1501 ttttatttca gcggttatgc agtgcaggcg gccgtttcgg cgtgtaagaa gtcgtttagt
     1561 ttttgcatat tctattattt aaattaaaaa acccggaaca tagtccgggt tcaataaaaa
     1621 tagtttattg tttgtcgaac tatgacacag ttgtatttat gttaatgtta ctcattgagt
     1681 gcaaatataa atacttttct gaaaatagtt caatactttt taaaaaaatt attcataagg
     1741 gcgaccggcc actttgaatg ttctggtaat attgaatcca aaacgaatat taaattcagg
     1801 tttgccattt actgtttcat aaatactgcc ttgtgttttt ccgatataat gttcaggaat
     1861 taaataaggg gaattagaaa attggagttg gaaaacgtgg cctttggtaa taaaatctgc
     1921 gcctattgat aaacaattat catatcccac aggcaattgg tcaggcaggg tataaaagta
     1981 ttcacctgtc aggtctatgt tttttgctac tctaactttg gtggcaattc caacagcaaa
     2041 aatatcattc gggtcgctgt ttaagtcaac taaattgcgg tgcacatgtg ttggcattaa
     2101 ctgcagtgaa aaaccatcgc taaatttacg cgcaattaat agctgattgg caaatccaat
     2161 tccaccggta aaataagggt ccaataattc atcggatgta tcggtaatta cactgatatc
     2221 actatacaat gtcaacgtaa taggcatgtt tttgtctccg gtgctttgtt ttagcagttt
     2281 tacttttaca aagccatcat aagttctggc caaattactt cttcctgctc caactgtaag
     2341 ccaattggtt ataccgtaat ctaagcctat acgcatatta gctccgccat taaaaccgaa
     2401 aaagttgtaa agtaaactga aagggttttg ctcataaata tctccaaaac ggtgggaaat
     2461 aataaacttt gcctgcccct gaatacttgt ttctacactt tgaccgctta tgatgcgggt
     2521 atcgcgaaaa gtttcagtgg cgtaaacggt attttgtgca cttagcaagc ctgaaattgc
     2581 cattagcacg aaaaaggtaa aagttctata catgaaatat attttaattg ttgagtgcac
     2641 ctgcattaat ccacgctctg attttggcaa ttttacaatc gcttaattga tctgagcctt
     2701 gtggcatttc tacatacccg gaaacccagg caactgtatt ataaaatgaa ctagcattgg
     2761 catcacctgc aactacataa tccatcaaaa aatcatatcc ttccaaaatc tggctgccgc
     2821 ctaaaactcc ggcatttgct tcactgtgac attctaaaca actagtttct aaaattggta
     2881 atatagttgt ggcgtaagtt acatcaactg tatcacaagc agtggttcca aataattcct
     2941 ctttactatc ataataacag gaattgaaaa aaaaggcaat acataacatg cctgcagcta
     3001 ttaagggtgt ctttttcatt ttaattgtta aggtatccat tttcaatcca aatgcgaatt
     3061 tcatctattt tgcaatcagg tagtggatta ccattatatg gcattccaac ataacctgct
     3121 tcaaacatta ttgttcctaa aaaacttcca tctgcagcaa tatccgcaac tccgctgtac
     3181 gaagataaat caattcctga agatgttgta gttgagttgt gacagccaag gcaataagta
     3241 tctacaattt taacaatttt gccgctataa gttactgctg tagtatcgca gtctcctcct
     3301 tcacatgcat tatttcttgc cccctgatta atccattttt ctaaaagtgc aatttgtgca
     3361 ttacttaaag gttcgtttgg agaaggtggc ataaaatctt caccaccatc tgtaaccgct
     3421 tctattagtt tgctgtcatt agcatcaaat gctttaacat aaccgctatt cattacatcc
     3481 tcataactcc caagtggtgc tttttcatca acacgaccaa tgccgtcatg acaaccactc
     3541 atcgcacaat tggaaatcaa taatggcaga atatcattct gaaaatatac ggtatccgga
     3601 tcgcaatcta cggttacagt tgggtttgtt aaatcaatga gtgtattgtg tttacatccg
     3661 gcaactatac ttgcaatgct taacgctaca attccgacaa attttgctgt tttcatttct
     3721 tttaatttac ttcttctcta atagtaacaa catgcaattg tccttaatcg tgcctgtata
     3781 tttgaaaatt aaaggtgact tttatcagtc ttcaggataa cctgcatcta cccaaatgcg
     3841 tatctggtca attaaacatt gtggcaacat agatcccgga ggcatagcaa caaatcctac
     3901 atcgccgttt aatgcgccca tcaatcgacc atctgaggca acatcaacta taccatcatt
     3961 tgagccggtt cctaaataag cagttaacga aattccacct gaaggagaac tggcatcgtg
     4021 gcagccggtg caataggcat tcataatggg tagaattgtt ccactaaagg taactgcact
     4081 agtatcgcaa tcggtgcaac tattgtctaa tgcaccctgt tcttgccaaa gtattattaa
     4141 ggcgatttct tcatcagtta aagaagccat tggcggaggt ggcattaaat catcacctgt
     4201 ttcggtcacc acatcataaa attcgctgtc gtaagggtcg cttggtttca taatatgact
     4261 tgaaatcacc ttggcatagg ttgataaatt gatgccttct tcatgagttt ctgcatcgtg
     4321 acaaccactt attgcacaat tggaaataag tatcggatag atatcatttt cgaaataaac
     4381 ggtatccggg tcgcaattag aagtgattaa tgttgtatcg ccaccgcccg ttgtgtctat
     4441 cggttcgata ggatcttcaa tcaaaatatc taaaggactg tgtttaaaag attggataga
     4501 tgctgcac
//
LOCUS       DAORKW010000281         4499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_898_length_4499_cov_5.646040, whole genome shotgun sequence.
ACCESSION   DAORKW010000281 DAORKW010000000
VERSION     DAORKW010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_898_length_4499_cov_5.646040"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /gene="meaB"
                     /locus_tag="PLB46_10855"
     CDS             complement(<1..838)
                     /gene="meaB"
                     /locus_tag="PLB46_10855"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRF77063.1"
                     /translation="MVNLNTQSPQYFFDGIRSGDRVLIARAITLLESTAQVQRDLGLE
                     ILQLCLPFSGNAIRIGITGAPGVGKSTMIEALGLHIAGQGHKLAVLAVDPSSKKTKGS
                     ILGDKTRMQELAKHPEVYIRPTASGEHLGGVAAATRETILLLEAANFDTVLVETVGVG
                     QSETAVHGITDVFILLLIAGAGDELQGIKRGIMEMADIIVMNKADGDNIQRSQMAMGE
                     VSRALHLFQEKESKWATTVSLGSAINGSGVAELWNSILAYQQHPRQNGFFDTNRSRQN
                     TTW"
     gene            complement(911..1633)
                     /locus_tag="PLB46_10860"
     CDS             complement(911..1633)
                     /locus_tag="PLB46_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF77064.1"
                     /translation="MRKLLTLIGVIAMASSVFAQSDSSAVNTVNFDDDDPSRDRIIMN
                     VHWDGWLGAPDSLNVKGLSSGMGFHFYYDIPLGSSTMKDNVSFAIGAGFNWSNYYNNS
                     YFSYDTSGNTFSTPFAEEVQFSKNKLVVNYVEVPLEFRFRTDEKNGNRFKLAVGFKGG
                     YVLSNHTKFVGDDYLSGSEDEIKYKQYRIKNMSNLQYGPTLRLGYSKVNIEAYYGLAP
                     LFVDGEGPTGTPITVGVSFNPF"
     gene            complement(1811..2401)
                     /locus_tag="PLB46_10865"
     CDS             complement(1811..2401)
                     /locus_tag="PLB46_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRF77065.1"
                     /translation="MNSATTTLNQLIRHRRSVKPGQYTGVPVSDDIVRQMLENARWAP
                     THALTEPWHFFVFSGEGLKKLADFQANTYKTNTPPEEFNELKYQKFLSGPLQASHIIV
                     VAMIRQVTQKLPIAEEISAVACAVQNMMLTATAFGIGSYWSTGGMTYKPEMKSFLGLG
                     ENDLCLGMVYVGNYTGEITDSTRKHIEEKSTWITEL"
     gene            2429..3532
                     /locus_tag="PLB46_10870"
     CDS             2429..3532
                     /locus_tag="PLB46_10870"
                     /inference="COORDINATES: protein motif:HMM:NF022818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HRF77066.1"
                     /translation="MITRKYYKITRVEAICLLLLVSVLFFSACKETAQNGTSNQDEVA
                     VAEKKVYDRWLIDLESVQSGCDRVDCIRSIDAPKFISVAATDYISDEDLVIGIKIGDE
                     VRCYPHNILNWHEIVNDAIGGNQFAINYCPLTGSGMAWNRNINGKITDFGVSGMLYNS
                     NVIPYDRNTRSAWSQMKQLCVNGELMNSYAETFQTIETTWATWKKLYPDSKVVSTETG
                     MPRDYMEYPYGEYRESRDITFDVEYENDTLHPKERLFGVIQKNKAKCYRFTNFKGGVR
                     IVNDTVFEKPIIVVGSERDNFITAFENPNQLKYLPVSNKLPAIIKDSNDNFYDVFGYC
                     VEGPEKGKRLTPMNGFMAFWFGWYAFYPDVTFM"
     gene            complement(3548..4081)
                     /locus_tag="PLB46_10875"
     CDS             complement(3548..4081)
                     /locus_tag="PLB46_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77067.1"
                     /translation="MKFYSILGLAAILTLGSCSAYLTPYTSSTQSKTNFGETELKKVQ
                     FYLDGDITLYRDVNSSDLEITSGEIKMIDGRQVEQIVIKSGTPGVIVKAENRDIFQVS
                     FDADGSFLRFGVNKDYNGRYTMMAKRWDGRVGIVDYAGKEYKSTPESIYAYLSVNMKK
                     IDNTSVESREAGGRTIE"
     gene            complement(4166..>4499)
                     /locus_tag="PLB46_10880"
     CDS             complement(4166..>4499)
                     /locus_tag="PLB46_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF77068.1"
                     /translation="GGKAGEVLNQLSDAEIATQLYPVMENCFPGITGLQTGKMARFYW
                     AGSPYAKASYACFAPGQYTTIEGAQREPSGNIYFAGEHCSFEFQGFMNGAAQTGREAA
                     EAILKAMS"
BASE COUNT         1359 a          894 c          830 g         1416 t
ORIGIN      
        1 accaggtagt attttggcga cttctgttgg tgtcaaaaaa accgttttgg cggggatgtt
       61 gttgataggc aagaatcgag ttccacaatt ctgcaacccc gctgccattt attgccgatc
      121 ccagggacac cgttgtagcc cacttcgatt ccttttcctg aaacaggtgt aatgctctgg
      181 aaacctcacc catagccatt tgtgagcgtt gaatattatc gccatcggct ttattcatca
      241 caataatatc ggccatttcc atgatgcctc tttttatacc ttgtaactca tcgccggcac
      301 cggcaattaa taataaaata aaaacatcgg ttatgccatg cacagcagtt tcgctttgtc
      361 caaccccaac tgtttctact aaaactgtgt caaaattggc agcttccagc aataaaatag
      421 tttctctggt agcagcagct acaccaccta aatgttcacc cgaagcagtt ggccgaatat
      481 acacttccgg atgtttggca agttcctgca tccgtgtttt atcacccaaa atactgcctt
      541 ttgttttttt gctgcttggg tcaacggcaa gcactgcaag tttatggcct tgtccggcaa
      601 tatgtaatcc aagtgcttca atcatggtgc tttttccaac acccggtgct cctgttatgc
      661 caatacgaat tgcatttcct gaaaatggta aacataattg caaaatctcc aatcccaaat
      721 cgcgttgcac ctgagcagta ctttccagca aggtaattgc tctggcaata agcaccctgt
      781 cgccggagcg tattccgtca aaaaaatatt gtggcgactg tgtattcaga tttaccatga
      841 taattctaca taaaaataaa aatccccccg ttgttaagcg gagggatact gtttatatct
      901 tgcatgtaat ttagaatgga ttgaacgaaa caccaactgt aataggggtt ccggttggtc
      961 cttcaccatc aacaaataaa ggtgctaaac cataataagc ttcaatattc acttttgaat
     1021 aacctaaacg caatgttgga ccatattgca gattgctcat atttttgata cgatactgtt
     1081 tgtatttaat ttcatcttct gaaccactca ggtaatcgtc gccaacaaat ttggtgtgat
     1141 tacttaaaac atatcctcct ttaaatccta cagccagttt aaatctgtta ccattttttt
     1201 catcagtacg aaaacggaat tccaaaggta cttccacata attcacaaca agtttatttt
     1261 tagaaaactg cacttcttca gcaaatgggg ttgaaaaagt atttccagag gtatcgtagc
     1321 taaaatagga attgttatag tagttagacc agttaaaacc ggcgccgata gcaaagctca
     1381 cgttgtcttt catggtagat gaaccgagcg gaatatcata ataaaaatga aaacccatac
     1441 cactagataa acctttaaca tttaaggaat cgggagcgcc tagccagcca tcccaatgca
     1501 cgttcattat tatcctgtcg cgggaaggat cgtcatcgtc aaaatttacc gtgtttactg
     1561 cactagaatc tgattgtgca aaaaccgaac tcgccattgc tataacacct atcagggtta
     1621 ataattttct catgtttgat ttgttatcga tgaaagtcat aaattagggg taaaattacg
     1681 taataattgc cattactatg aaaactaatt ccttaactgc attacttaac gtttgtttag
     1741 tggcattcat tttatcacta aaccaaatat ttggacaata tcatttctac catactcgat
     1801 ttttttaaaa ttataattca gtaatccagg tggatttttc ctcaatatgt tttctggttg
     1861 aatcggtaat ttctcctgtg taattgccca catacaccat acccaaacac aaatcgtttt
     1921 caccaagtcc taaaaacgat ttcatttcag gtttataggt catgccccct gtgctccagt
     1981 aactgcctat accaaaagcg gtagccgtaa gcatcatatt ttgtacagca caggcaacag
     2041 cactaatttc ttccgcaata ggcaattttt gcgtaacctg acgaatcata gcaacaacaa
     2101 taatatggga agcctgcaaa ggacctgata agaatttttg atatttcaat tcgttaaact
     2161 cttcaggtgg tgtatttgtt ttgtaggtat ttgcctgaaa atctgccaac ttttttaatc
     2221 cctcaccgga aaaaacaaaa aaatgccaag gttctgttaa ggcatgtgta ggcgcccatc
     2281 gggcattttc gagcatttga cggacgatat catcacttac aggaacacct gtgtattgcc
     2341 ccggttttac tgatcttctg tggcgtatga gttgatttaa ggtagtagtt gctgaattca
     2401 tataaagtcg aattaaattt gcactgaaat gataacgcga aagtattata aaataacgag
     2461 agtagaagct atttgtttat tgctgctcgt tagtgtatta tttttttccg cctgtaaaga
     2521 gacagcacaa aatggaacat cgaatcagga tgaagtagcg gttgcggaaa aaaaagtgta
     2581 cgaccgctgg ttaattgatt tagaaagtgt gcaatcaggt tgcgacagag tagattgtat
     2641 tcggtcgatt gatgcgccta aatttatttc ggttgcggcg acagattata ttagtgatga
     2701 agatttagtt attggaatta aaattggtga tgaagtgcgt tgttatccgc acaatatttt
     2761 aaactggcat gaaatagtta atgatgcaat tggtggcaat caattcgcta ttaattattg
     2821 cccactcacc ggttcaggaa tggcctggaa tagaaatatt aatggtaaaa taaccgattt
     2881 tggtgtttca ggaatgttat acaacagcaa tgtaattcct tacgatcgca atacacgctc
     2941 tgcgtggagt caaatgaaac aactatgtgt gaatggtgaa ttgatgaaca gctatgctga
     3001 aacatttcaa acaatagaaa ctacctgggc aacatggaaa aaattatatc ctgatagtaa
     3061 agtagtatct acagaaaccg gcatgccacg cgattacatg gaatatcctt atggcgaata
     3121 tcgtgaatca agagatatta cttttgatgt tgaatatgaa aacgacacct tacatcctaa
     3181 ggaaaggtta tttggtgtaa ttcagaaaaa taaagcaaaa tgttatcgtt ttaccaactt
     3241 taaaggtggt gttcgcattg taaacgatac ggtttttgaa aaaccaataa ttgttgttgg
     3301 cagcgaacgc gataatttta ttacagcatt cgaaaatcct aatcaactaa aatatttacc
     3361 ggtatcaaat aaattacctg ctattattaa agatagcaat gataactttt atgatgtatt
     3421 tggatattgc gttgaaggtc ccgaaaaagg taaacgttta actccaatga atggatttat
     3481 ggcattttgg tttggctggt atgcctttta tcctgatgtt acatttatgt aaaaattatt
     3541 gtaacattta ttcaatagtt ctaccacctg cttcacgact ttcaacagaa gtattatcta
     3601 tttttttcat gtttaccgat aagtaggcat aaatactttc aggtgtcgat ttatattctt
     3661 tacctgcata atctacaata ccaactctgc cgtcccaacg ttttgccatc atggtatatc
     3721 ttccgttata atctttattt acaccaaaac gtaaaaagct gccgtcagca tcaaaactta
     3781 cctggaaaat gtcgcggttt tctgctttta caattacacc cggtgtaccg gatttaatta
     3841 caatttgttc cacctgtcgg ccatcaatca ttttaatttc tccggaggta atttctaaat
     3901 cgctgctatt tacgtcgcgg tacaacgtaa tatcaccatc taaataaaac tgcacttttt
     3961 tcagttctgt ttcaccaaaa ttagttttgg actgggttga tgatgtgtaa ggcgttaaat
     4021 aagccgaaca tgagcctaat gtaagtatag ctgctaatcc tagaatagag taaaatttca
     4081 tattgaggtg ttttttgtgc tagaaatcaa taataatgcc ataggtgtaa tgacggtgca
     4141 attttagtta cttataggca ttatttcaac tcatagcctt caaaatggct tctgcagctt
     4201 ctcttccggt ttgcgcagct ccgttcataa acccctgaaa ctcgaagctg caatgttcgc
     4261 cggcaaagta aatattgccc gaaggttcgc gctgggcacc ttcaatggtg gtgtattggc
     4321 ctggtgcaaa acaggcataa ctcgctttag cgtaaggact gcccgcccaa taaaatctag
     4381 ccatttttcc ggtttgtaaa ccggttatgc ctggaaaaca attttccatt acaggataca
     4441 attgtgttgc aatttcagca tcgctcaatt gattgagcac ttcgcctgcc tttcctccg
//
LOCUS       DAORKW010000282         4499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_899_length_4499_cov_5.347210, whole genome shotgun sequence.
ACCESSION   DAORKW010000282 DAORKW010000000
VERSION     DAORKW010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_899_length_4499_cov_5.347210"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLB46_10885"
     CDS             <1..373
                     /locus_tag="PLB46_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF77069.1"
                     /translation="TEIEKDIYIYGDRFSFSSVIYNLIENAIKYSPADAEITVALTGD
                     EKNAYLSVKDTGIGIPGSEKSRIFERFYRIGTEETRKTKGTGLGLFIVKHVVEMHKGS
                     IQVKNNSPKGSIFEVSIPVGA"
     gene            454..1146
                     /locus_tag="PLB46_10890"
     CDS             454..1146
                     /locus_tag="PLB46_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF77070.1"
                     /translation="MNYRILIVEDEDHIRENLKLNLELEGYEVIAVADGAKAIKAFKE
                     QRFDLVILDVMLPEMDGFDVCEQIRLDDRETPILFLTAKDASIDKIKGLKTGGDDYMT
                     KPFNLEELLLRIINLLKRSSKVPAEDKFHAYEFGGNTINFVTYEAKGINGDVKLTNKE
                     LKLLKLLIDRKNEVVSRTLILQTVWGYDVYPSTRTIDNFILAFRKYFEKDPKNPTYFH
                     AIRGVGYKFSDK"
     gene            1254..>4499
                     /locus_tag="PLB46_10895"
     CDS             1254..>4499
                     /locus_tag="PLB46_10895"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF77071.1"
                     /translation="MQNLQKVLFLIIIFCLSKVSTIGQVVINEGSNKNGNTIADEDGD
                     FESWIELYNAGAIDINIGDYGVSDDSSLLNKWTLPDYYLGAGQFLLLFTSGKNRKPDT
                     DINHWEQPINSGSVWKYIIPNAFIPATWINTDFDDDTWSSGLCSVGYGDDDDNAVVPD
                     ATRSVYLRQSFTIADTALLGDALFSIDYDDGFIAYVNGNVIALNGFAAGSPAYNEFSS
                     IDHEAAMYTGGNPEHFTIDEALIKSYLVEGENILAVEVHNVTAGSSDLTALPFLSFAV
                     KDEMIIWTDVLPAWFPTTTLTANLHTNFKINAEGEKIYLSNPDGISIDTLLVNVEFAD
                     HSIGCTTDGAATNGIFMVATPGASNSGAFYEGYTDGVATFTLDAGFYTGSQITSINVP
                     PGTAVHYTLNGEIPTLADPLYTAPITIDETQVIKARVFDLAGELLPGQISTNTFFIDE
                     SISVPVISISTNNDNLYGGFGIFDNWWNDWKKYAHVEYFDSLHVNAFEQAVGIKVDGG
                     AGGSRSLPQKSMRVELDNAAFGDGELHYPLLKRRSWVEEYETFYLRNGSNMSNVLPYK
                     DAFMVRTTEGTYNEHMAYEPVVIYINGEYWGLYELRNKLDEGHFENAKGVEGDSLDLL
                     TLSYWYGLVLRTLSGSDTDFIAMRNYLGTYPTPEDPEFYYIADSLLDLQNFTDYIAAQ
                     MFFANYDWPYNNIKAWRDRGGDNKWKYALIDVELGLGIGGWSDANADLIPGLFNPQEH
                     IEPLYNLLKNPIYHDYFVNRYADLMNSTFLPERTLAMEDSIYNEVVAEMPRQLELWGS
                     GTIDEQMATFENYREALRDDFDVRANKERTHIKNGFDLNDKVEITLTCSPTGAGYIKI
                     STLTIFDMPWTGTYFDGVPVQITAYPNTGYTFSHWDDSEFIDDVMANGFLSNMDNDVT
                     FTANFIGAPAPSLVAINEINYNPEATVNAGNWFELWNYGIADVNISGWKVRDADFLHE
                     FIFPENTWIPADGRLVLAVDTTLFFAQHPDVTNVMGPLGFGLNNATETIKLINLKNEV
                     VQEIQYTDDAPWPGGADGQGRTMERTNPLVAENIPSNWFDGCIGGSPGLPYT"
BASE COUNT         1454 a          748 c          896 g         1401 t
ORIGIN      
        1 aactgaaatt gaaaaggaca tttatattta tggcgaccga ttttcgtttt cgtctgtgat
       61 atataacctt attgaaaatg caattaaata ttcacctgca gatgccgaaa ttaccgttgc
      121 attaacaggc gatgaaaaaa atgcttacct cagtgtaaaa gacaccggta ttggcattcc
      181 aggttccgaa aaatctcgaa tatttgaacg attttatcgc atcggaacag aagaaacacg
      241 caaaaccaaa ggaacaggac tagggttatt tattgtaaaa catgtagtag aaatgcataa
      301 aggttctatt caggttaaaa ataattcccc taagggttct atatttgaag tgtcaattcc
      361 tgttggggct taaaaacgac aaaatgatga catttttaag caaacccaaa tgtagaagtg
      421 ccgtataaca ggatataacc caaaactcta actatgaact atagaatttt aatcgttgag
      481 gacgaggacc acatccgcga aaacctcaaa cttaaccttg aactcgaagg ttatgaagta
      541 attgcagttg ctgatggcgc taaagcaatt aaagctttta aagaacaacg tttcgattta
      601 gtaattcttg atgtcatgct gcctgaaatg gatggttttg atgtttgcga acaaatcaga
      661 ctcgacgacc gtgaaacgcc aattttattt ttaacagcaa aagatgcttc tattgataaa
      721 attaagggcc ttaaaaccgg tggtgatgat tatatgacta aaccatttaa tctggaagag
      781 ttgttactga gaattatcaa tctgcttaaa cgctcatcta aagttcctgc ggaagataaa
      841 tttcacgcct acgaatttgg cgggaatacc atcaattttg tcacctatga agcaaaaggt
      901 attaacggag atgttaagct gacaaacaaa gagcttaaat tattaaaatt gttaattgac
      961 cgcaaaaacg aagtggtatc acgcacactc attctgcaaa ccgtttgggg atacgatgtg
     1021 tatccttcaa cacgaacaat cgacaacttt attcttgctt ttagaaaata ttttgaaaaa
     1081 gacccaaaaa atccaaccta ttttcatgca ataagaggag ttggatacaa attctcagac
     1141 aaataagcga atatccacct gtaaccaata aacgccttga accatatctg attcagggcg
     1201 tttttgttaa ttgttgctta tatttgttaa ccaaaccact cagcacacac gttatgcaaa
     1261 acctccaaaa agtattattt ctaataatta tattttgttt aagtaaggtt tcaacaatag
     1321 gccaagttgt tataaacgag ggctccaata aaaatggtaa taccattgct gacgaagacg
     1381 gcgattttga atcctggatt gaattgtata atgctggtgc tatcgatatc aatatcggcg
     1441 attatggtgt ttcagatgat agtagtttac tgaataagtg gaccttaccc gattattacc
     1501 ttggagcagg tcaattttta ttattattta cttcaggtaa aaaccgaaaa cctgacaccg
     1561 atattaacca ttgggaacaa cccattaatt ctggttcagt gtggaaatat attattccca
     1621 atgcatttat acctgcaaca tggattaata ccgattttga tgacgatacg tggtcatcag
     1681 gattatgcag tgtgggttat ggcgatgatg atgataatgc tgtagtacca gatgcaacgc
     1741 gaagtgttta tttacggcaa tcattcacca ttgctgatac agctttatta ggtgatgcac
     1801 tttttagtat cgattatgat gatggattta ttgcttatgt taatggaaat gttattgcac
     1861 tcaatggatt tgccgcaggt tctcctgcct acaacgaatt ttcaagtata gatcatgaag
     1921 cggcaatgta tacaggcggc aacccggaac attttactat tgatgaagcc ttaattaaat
     1981 cgtatttggt tgaaggcgaa aatattcttg ctgtagaagt acataatgtt acagccggtt
     2041 cttccgattt aacagcttta ccatttttat cattcgcagt aaaagatgaa atgattattt
     2101 ggacagatgt acttccagcc tggtttccta caaccacatt aactgctaat ttgcacacta
     2161 attttaaaat taatgcagaa ggtgaaaaaa tttacctttc taatcctgat ggaatttcta
     2221 tcgatacctt attagtaaat gttgaatttg cagatcatag cattggatgt actactgatg
     2281 gtgcagcaac aaacggaatt tttatggtgg caactcctgg cgcatcaaat tcaggcgctt
     2341 tttatgaagg ttacacagat ggtgtcgcca catttacttt agatgcagga ttttataccg
     2401 gttctcaaat cacaagcata aatgtgccac cgggaacagc agtgcattat accttaaatg
     2461 gtgaaattcc aaccttggca gatcctttgt atacagcacc aattaccatt gatgaaacac
     2521 aagtcataaa agcgagagta tttgatttgg ctggtgaatt acttccagga caaatcagca
     2581 ccaacacatt ttttattgat gaatcaattt ctgttccggt aatttcgatt tcaaccaata
     2641 atgataattt atatggtggt tttggcattt ttgataattg gtggaacgat tggaaaaaat
     2701 atgcacatgt tgaatatttt gattcgttac atgttaacgc ttttgaacaa gctgttggaa
     2761 ttaaagttga tggcggcgca ggaggtagta gatcgctacc gcaaaaaagt atgcgtgttg
     2821 aattagataa tgctgcattt ggcgacggtg aattgcatta tcctttatta aaacgaagaa
     2881 gttgggttga agaatatgag acattttatt taaggaatgg aagtaatatg agtaatgtgc
     2941 taccgtacaa agatgctttt atggttcgca caaccgaggg cacttataat gaacacatgg
     3001 cttatgaacc cgttgttatt tatataaatg gtgaatattg gggtttatat gaattgagaa
     3061 ataaattaga tgaagggcat tttgaaaatg ctaaaggtgt tgagggtgat agtttggact
     3121 tattaacttt gagttattgg tatggtttgg tattgcgcac actcagtggt tctgataccg
     3181 actttattgc tatgcgcaat tatttgggta cttatccaac tcctgaagac cctgaatttt
     3241 attatatcgc agacagttta ctcgatttac aaaactttac cgattatatc gcagcacaaa
     3301 tgttttttgc taattacgat tggccatata ataatattaa agcatggcgc gatagaggtg
     3361 gagataataa atggaaatat gcattaatag atgtggaact cggacttggt attggaggct
     3421 ggtcagatgc taatgcagat ttaattcccg ggttatttaa cccgcaagaa catattgaac
     3481 cactttataa tttattgaaa aatccaattt accacgacta ctttgtaaat cgttacgctg
     3541 atttaatgaa cagcacattt ttacctgaac gtacattggc aatggaagat agtatctaca
     3601 atgaggtggt agctgaaatg cctcgccaat tagaactttg gggttcaggt acaattgatg
     3661 aacaaatggc aacttttgaa aattatcgcg aagcacttcg tgatgatttt gatgtaagag
     3721 caaataaaga acgaacacat attaaaaatg gatttgacct caacgacaaa gttgagataa
     3781 cactaacctg ctcacctaca ggagctggtt acattaaaat aagtacactc actatttttg
     3841 atatgccttg gactggaact tattttgatg gtgtgccggt tcaaattacg gcctatccaa
     3901 ataccggtta tacattttct cattgggatg atagcgaatt tattgatgat gtaatggcta
     3961 atggtttttt atccaacatg gataatgatg ttacatttac tgctaatttt attggagctc
     4021 ctgcaccatc gttagttgca ataaatgaaa tcaattataa tccggaagct acagtgaatg
     4081 ccggcaactg gttcgaatta tggaactatg gaattgctga tgtaaatata tccggatgga
     4141 aagtgagaga tgctgatttt ttacatgaat ttatttttcc ggaaaatacc tggatacccg
     4201 cagatggaag actggtttta gcagttgata ccactttgtt ttttgcgcag catcctgatg
     4261 ttaccaatgt tatgggacca cttggcttcg gtttaaacaa tgcaacagaa acaattaagt
     4321 tgataaattt aaaaaatgaa gtagtgcagg aaattcaata tacagatgat gctccatggc
     4381 cgggtggtgc agatggacaa ggaagaacga tggaacgcac taatcctctt gtggctgaaa
     4441 acatcccttc aaattggttc gatggttgta ttggcggttc accgggatta ccttatacc
//
LOCUS       DAORKW010000283         4475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_907_length_4475_cov_5.333484, whole genome shotgun sequence.
ACCESSION   DAORKW010000283 DAORKW010000000
VERSION     DAORKW010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_907_length_4475_cov_5.333484"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..>852
                     /locus_tag="PLB46_10900"
     CDS             70..>852
                     /locus_tag="PLB46_10900"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HRF77072.1"
                     /translation="MRRYFNLILALVIVGISNRAIAQITWTTGYTATEIVETLAGDGV
                     EVDNATIDCHNLAFGQFDCVDCNVGIDSGIILTSGRVSNIAGPNNLGSTGTSNAWAGD
                     PDLNALPGITVTHDACVLEFDVYSPGDSLKFEYVFGSEEYPEYANSSINDCFAFWISG
                     PGYATPTNIALIPGTVEPVTIANVNNVDHPEYYIANGTGGVGVFATDPYYIEYDGFTV
                     VLTAEAAVSPCNWYHLKIALADEEDYSWDSGVFLRAESLTTTY"
     assembly_gap    853..862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <863..2917
                     /locus_tag="PLB46_10905"
     CDS             <863..2917
                     /locus_tag="PLB46_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF77073.1"
                     /translation="TYLADFTFPGYDFGEDAIFCTTEPDPDPVLAAGAETGTFTCAPI
                     GLDFNPGTGELDLSDSEPGDYVLTNTLITGFCDLDTFVYTMNITIEEPGVAGFLFPGS
                     PYCDNETDPSPVLVAGGMLGTFTSSPAGLVINPVTGVVDLDASSPGLYTVTNTVAAGV
                     VCPGASATASITIHPTYDIIIDAAICLGDSYTLPDGGVVFTSGTYVANLTTAKGCDSV
                     ITTNLIVNPVYNYTLTPNICDGSSYILPDGTAVTDAGTYINAFTTAAGCDSIYTVTLT
                     VNPVPTTNHTVHLCDGETFTLPDGTDVGVTGFYPVTLVTADGCDSTVNTTLLVHPVYD
                     IVFNPIICDDETYTLPDGTVVNTPGVYVNNFLTDKDCDSIITTNLVVNPTYDLIYNPE
                     ICIGETYTLPDGSTADASGTFNYFLTTGEGCDSNITVNLTVHPLPVLTWELDDIICLE
                     EGSVLMEAIPSGGTYSGPGIFGDNFVTATAGVGGPFTLTYEYTDANGCYNSITAETSV
                     DDNYAIAWGDTSIYYGEDAVLYSEAGGDYTWSPSDGIACITCPETNVIPPFTTNYVMT
                     SIDENGCIGTDNVLVTILADPPNEIFVPNTFTPNGDNINDMFFAFGWNITNITSMQVF
                     DRWGELVFFVENVMPGDISKGWDGKINGLDANNGVYVFIVQAQFETGQYMTKSGNVTL
                     LR"
     gene            complement(2975..>4475)
                     /locus_tag="PLB46_10910"
     CDS             complement(2975..>4475)
                     /locus_tag="PLB46_10910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF77074.1"
                     /translation="YHRGKTFCNFAVMANVLISNTYFLALDPKQQDNMQPYPPLGALY
                     AAAVLERDGHHVEFIDTIFEPSAASVFPTIKQFSGNIFFICDDGFNYLTKMCLVNMRN
                     ACYDMIAYAKKNNYKVIVSSSDATDHSLEYLEHGADFIIIGEVEVTLQQLINALQSKS
                     TDFDTINGIAYLHESELIKTVPRPVLHDLDSLPLPAWKLIQMDVYKNAWINRSGYFSL
                     NMTTTRGCPFKCNWCAKPIYGNRYNSRSPEQVVEEIAFLIKNYQVEHIWFCDDIFGLK
                     PGWIKQFSELVVSKNLQFKFKIQCRADLLLDDENIHHLAIAGCDEIWIGAESGSQKIL
                     DAMDKGTTVEQIYTATRLMQKHGINPCLFLQFGYLGELQSDIDLTLKMVFDLMPHDIG
                     ISVSYPLPGTKFYEKVKSQLNIKQNWTDSDELLLMYKGTYSPAYYKQLHRYIHKKFRL
                     AQGKKYFRSLLKGERKPNKTITRRILTMPLFFVAAIWHKRNMDLLSKQQ"
BASE COUNT         1292 a          826 c          853 g         1494 t
ORIGIN      
        1 ggttagtttg tttgtaattt tttgcctttt tgagggtaaa catcaaccaa aatcaagcac
       61 acatttggta tgcgccgtta ttttaatctc atattagctt tggtaatagt tggaatttcc
      121 aaccgagcca ttgcacaaat aacctggaca acgggttata ccgcaactga aatagtggaa
      181 acccttgccg gcgacggcgt tgaagtagat aatgctacaa ttgactgtca taatcttgca
      241 tttggacaat ttgattgtgt agattgtaat gtgggtatag atagtggaat tattttaacc
      301 tccggccgcg tttcaaatat tgccggtcct aataatttag gtagtaccgg caccagtaat
      361 gcatgggccg gcgacccgga cttgaatgcc ttacctggaa ttaccgtaac tcatgatgct
      421 tgtgttttag aatttgatgt ctattctccg ggtgatagtt taaaatttga atatgttttt
      481 ggttctgaag aatatccgga gtatgcaaat agctctatta acgactgttt tgccttttgg
      541 ataagcggcc ccggatatgc caccccaaca aatattgctt taataccggg cacagttgaa
      601 ccggtaacca ttgccaatgt aaataatgtg gaccacccgg aatattacat tgccaatggg
      661 actggtggtg tgggggtttt tgctaccgac ccttattata ttgaatacga tggatttaca
      721 gtagtgttaa ctgctgaagc tgcggtttcg ccatgtaact ggtaccattt aaaaattgca
      781 cttgctgatg aggaagacta ttcatgggac tcaggtgttt ttttacgagc agaaagttta
      841 acaaccacat atnnnnnnnn nnacatattt ggctgacttt actttccctg gttacgattt
      901 tggtgaagat gctattttct gcactaccga acctgaccct gaccctgttc tagccgccgg
      961 tgctgaaacc ggaacattta cctgcgctcc gattggttta gattttaatc cgggaactgg
     1021 tgaattagac ttaagtgata gtgaacccgg agattatgtt ttaaccaata ctttaattac
     1081 cggtttctgc gatttagaca ctttcgttta tacaatgaat attacaattg aagaacctgg
     1141 tgtggctggt tttttattcc ctggttctcc ttattgcgat aatgaaaccg acccttctcc
     1201 tgttttagtt gcagggggaa tgttaggcac gtttacttca tcacctgctg gtcttgtaat
     1261 taatccggta actggagttg ttgatctaga tgcgtcttct cctggccttt acactgtaac
     1321 caatactgtt gcagccggtg ttgtttgtcc gggtgcttct gcaacagcat ccattaccat
     1381 tcatcccact tatgatatta taatagatgc cgcaatttgt ctcggcgatt cctatacttt
     1441 acccgatggt ggagttgttt tcacaagtgg aacctatgtt gctaatttaa caacagctaa
     1501 aggttgcgat tcagtaatta ctactaacct aattgtgaat ccggtatata attatacttt
     1561 aacgccgaac atttgcgatg gttctagtta tatattacca gatggaacgg cagttactga
     1621 tgccggaact tatattaacg cgtttactac agctgccggt tgcgattcaa tttataccgt
     1681 aacattaact gttaatccag tacctactac taaccataca gttcatcttt gtgatggcga
     1741 aacttttaca ttgcccgatg gaaccgatgt aggcgttaca ggtttttatc ctgttacttt
     1801 agttacagca gatggatgtg attcaaccgt aaataccaca ttattagttc atcctgttta
     1861 cgatattgta tttaatccaa ttatttgcga tgatgaaaca tatacattgc ctgatggaac
     1921 tgttgtaaat acgcctggag tttatgtaaa taatttttta actgataaag attgcgactc
     1981 tattatcaca acaaatttag tcgtaaatcc tacttacgat ttaatttaca atcctgaaat
     2041 ttgtattggt gaaacataca ctttacctga tggtagtaca gctgatgcaa gtggaacctt
     2101 taattatttt ttaacaaccg gtgaaggttg cgattcaaat attactgtta atttaaccgt
     2161 acatccgctg ccagttttaa cttgggaatt agatgatatt atttgtttgg aagaaggttc
     2221 agtattaatg gaagcaatac catccggcgg aacttatagc ggccccggaa tatttggcga
     2281 taatttcgta actgctactg ccggagtagg tggccctttc acccttactt atgaatatac
     2341 cgatgcaaat ggttgttata atagcataac tgcagaaact tcagttgacg ataattatgc
     2401 tattgcctgg ggtgatacta gtatatacta cggtgaagat gctgttttat acagcgaagc
     2461 gggtggtgac tatacctggt caccttctga cggaattgcc tgtattacgt gtcctgaaac
     2521 aaatgttatt ccgccattca caactaatta tgtgatgaca tccattgacg aaaatggttg
     2581 tattggtacc gacaatgttt tggtaacaat tttagctgat ccgccaaatg aaattttcgt
     2641 tccgaatact tttaccccta atggtgataa tataaacgat atgtttttcg catttggatg
     2701 gaatattaca aatattacct ctatgcaagt tttcgatcgt tggggtgaat tagtattttt
     2761 tgtagaaaat gtaatgcctg gagatattag taaaggatgg gatggaaaaa ttaatggctt
     2821 agatgccaat aatggtgttt atgtgtttat tgttcaggcg cagtttgaaa ctggtcagta
     2881 catgacaaaa tcaggaaacg taacactact tcgataatta aataatttta aatgaactcg
     2941 tgattgtata ttgaaaaata tatcacgagt ttttttattg ctgcttactc agcaaatcca
     3001 tattacgttt atgccaaatt gcagcaacaa aaaacaatgg catagttaaa attcttcggg
     3061 taattgtttt gttcggtttc ctttcccctt tcaatagtga acgaaaatat ttttttcctt
     3121 gcgccaatct gaattttttg tgaatataac gatgtaattg tttgtaataa gctggcgaat
     3181 aagtgccttt atacattaac aataattcat cactatctgt ccagttttgt ttgatgttta
     3241 attgcgattt tactttttca taaaatttgg ttccgggcaa tgggtaagaa acagaaatac
     3301 cgatgtcgtg aggcattaaa tcaaatacca tctttaaggt gaggtcaata tctgattgta
     3361 attccccgag gtaaccaaat tgtaaaaata aacaagggtt aattccatgc ttttgcatta
     3421 gtcttgtagc ggtataaatt tgttctacag ttgttccttt gtccatagcg tcgagaatct
     3481 tttgagaacc gctttctgcg ccaatccaaa tttcatcgca acctgcaatg gcaaggtgat
     3541 gaatattttc gtcgtctaat aataagtccg cccgacattg aatcttaaat ttaaattgca
     3601 agtttttact tacaacaagt tcactaaatt gttttatcca accgggtttt aatccaaaaa
     3661 tatcatcgca aaaccaaata tgttcaacct gataattttt tattaaaaac gcaatttctt
     3721 ctaccacttg ttccggtgaa cggctattat acctattacc ataaattggt ttagcacacc
     3781 agttacattt gaacggacaa cctcgtgttg ttgtcatatt cagtgaaaaa tacccgcttc
     3841 gatttatcca tgcatttttg taaacgtcca tttgaattaa tttccatgcc gggagtggca
     3901 ggctatccaa atcgtgtaaa acaggccttg gaaccgtttt aatcaattca ctttcatgca
     3961 aataagcaat gccattaatt gtatcaaaat cggttgattt agattgtaga gcattgatta
     4021 attgctgtaa ggtaacttct acttcaccta tgatgataaa gtctgcccca tgttctaaat
     4081 attccaaact atggtcggta gcatcactac tggaaactat tactttataa ttattttttt
     4141 ttgcataggc aatcatgtcg taacaggcat tacgcatatt taccaaacac atttttgtaa
     4201 ggtaattgaa accatcatca caaataaaaa atatattgcc ggaaaattgt ttaatggtcg
     4261 gaaaaacact cgctgctgaa ggttcaaaga tagtatcgat aaactcaaca tgatgcccgt
     4321 ccctttccaa cacagcagca gcatacaagg cgcccaacgg tggataaggc tgcatattat
     4381 cttgctgctt agggtctaat gccaaaaaat aagtattgct gatgagcaca tttgccatta
     4441 cagcaaaatt acagaatgtt tttcctctat ggtac
//
LOCUS       DAORKW010000284         4443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_916_length_4443_cov_3.750684, whole genome shotgun sequence.
ACCESSION   DAORKW010000284 DAORKW010000000
VERSION     DAORKW010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_916_length_4443_cov_3.750684"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2197)
                     /locus_tag="PLB46_10915"
     CDS             complement(<1..2197)
                     /locus_tag="PLB46_10915"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HRF77075.1"
                     /translation="MKNLFILSILFGSLQLSATEYYVSPSGNNANAGTSTAAPWKTIQ
                     FALNMANDGDVINLMAGTYTGKVTWNDGGSAGNYITLQNYNTDIVILDGSTVGNSQAM
                     MYIENKDYIKIDGLKFTNHNGNYQPIINLYGNNNHIEISNNEFYNTDCNESYAILCEG
                     RGDDIKITNNNMHDLIGDNAVGVLFVGSNTTTAFTNIVISGNTLTNLDPAPSEAIAVN
                     GNVDGFEISNNILNDINNIGIVMIGGEDWVNTNDAVNFARNGVCKNNSVTGANSIYGG
                     GFAGGVYVDGGKDIIVENNIITGSDVGLEIGCENAGFIAENIIVRNNIIYKNEKAGLG
                     FGGYDFPATGQVQNCVFTGNTVYDNDILNTGFGQLWIQYALNCVVENNIFYTSTNAWM
                     VNAETINSSYNNVLNYNDYYYPAGMNNAKFFFDFDYIVGYTNFQTATGEDVNSITANP
                     LFVNELAAIPDFHLSPSSPCKDAGNPTYDDLGIDVDDVDMDGTNRVNGTAIDMGADER
                     VILPLSWSVSSTNVTCYGSCNGVLSFNGVEGCGTYTLEYKNPGGGVWIPYTAPVTGLC
                     AGTYKIRITDNCGSVVMGNVALTQDPVLNVSVTSIVNETVAGASNGKITVNSTGGFGV
                     KQYSKNGGFTWQTSKKFNGLTAGTYTILVKDAHECLDEVAATVGIGLKEGYTENFNLY
                     PNPASDAVTININGLTAPGKLYCYNILGECMFITILTQEESTIDVSYLPA"
     gene            complement(2281..3033)
                     /locus_tag="PLB46_10920"
     CDS             complement(2281..3033)
                     /locus_tag="PLB46_10920"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HRF77076.1"
                     /translation="MKNALLKTMLVVFIQMQHVLAFPPANPNVDPYYGNSTFEIKSQG
                     ANFITVAIDGDFFNEPVKKFAVNNMAPGNHYVEVFTDKIQHSGYYATTQRVRLYAGYV
                     YIKPASLVHGVIDNFGRFYVKSVQALQMYEPVYYQPVYPDYPPYQQPQQPVCNAQQQM
                     NAASFNALIQTIHNQWFDDTKLNVARQAVQSGWFTSEQIATMMREFSFEDSKLELAKT
                     AYSHVVDKQNYFVVYDEFWFSSSVDELIRFIH"
     gene            complement(4048..>4443)
                     /locus_tag="PLB46_10925"
     CDS             complement(4048..>4443)
                     /locus_tag="PLB46_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRF77077.1"
                     /translation="NASNISIVNEPDTFYHLRDKLKNLDVKPVKTKVANIVEYRIRSL
                     QIKKYALERAMGICEYCQNTAPFIDSNGIPFLEVHHIHKLADDGPDIPINVGAICPNC
                     HREAHYGINKTRINEKLITIIKTKEDGLD"
BASE COUNT         1398 a          847 c          760 g         1438 t
ORIGIN      
        1 ctgcaggcaa atacgaaaca tctatcgtag attcctcctg cgttaaaatg gtaataaaca
       61 tacattcccc taatatatta tagcaataca acttaccggg tgctgttaaa ccatttatat
      121 taattgtcac agcatccgat gcaggattgg gatacagatt aaaattttcg gtataaccct
      181 cttttaaacc tattccgaca gtcgcagcaa cctcatccaa acattcatgt gcatctttaa
      241 ctaatatggt gtaagtccct gcagtcaatc cattaaattt tttactagtt tgccaggtaa
      301 atcctccatt ttttgagtat tgttttacac caaatccacc tgttgaattc acggttattt
      361 ttccattgga agctccggca actgtttcat tcacaatact ggtaaccgat acatttaaca
      421 cagggtcttg tgtgagcgca acattgccca ttactaccga accacaatta tcggtgatac
      481 gaattttata tgttccggca cataatcctg ttacgggtgc ggtataagga atccaaacac
      541 cacctcccgg gtttttatat tccaaagtat aagtgccaca accttctaca ccattaaaac
      601 tcaatactcc attacatgaa ccgtaacaag taacattcgt actgcttacc gaccaactca
      661 atggtagtat cacacgttcg tctgcaccca tatcgatggc agtgccgttt acgcgatttg
      721 tgccatccat atccacatca tcaacatcaa tacctaaatc atcgtaagtg ggattgccgg
      781 catctttaca aggtgaactt ggtgataaat gaaaatcagg aatggcagca agttcgttga
      841 caaacaaagg attagcagta atggaattta catcctctcc tgttgctgtc tgaaaattag
      901 tataacctac aatataatcg aagtcaaaaa agaatttcgc attattcatt cctgcgggat
      961 aataataatc gttataattt aacacattat tataactcga attaattgtc tcggcattta
     1021 ccatccaggc attggtagat gtataaaaaa tattattttc taccacacaa tttaatgcat
     1081 attgaatcca taattgtccg aagcccgtat ttaaaatatc attatcataa accgtattgc
     1141 cggtaaatac acaattttgc acctgccctg tcgccggaaa atcatatccg ccaaaaccta
     1201 aacctgcttt ttcatttttg tagatgatat tatttctaac aataatattt tcggcaataa
     1261 atcccgcatt ttcacaacca atttcaaggc ctacatcgct gcctgtgatg atattatttt
     1321 ctacgataat atctttccca ccatccacat acacgcctcc ggcaaatccg ccaccataaa
     1381 tagaattggc acctgttaca ctattatttt tacaaacacc atttcgcgca aaatttacag
     1441 catcgttggt atttacccaa tcttcgccgc caatcataac aataccgata ttattaatat
     1501 catttaaaat attattgctg atttcaaatc cgtccacatt accattcact gcaatggctt
     1561 cacttggagc agggtctaaa ttggtgaggg tatttccgct aattacaata ttggtaaatg
     1621 cagtcgtggt attcgaaccg acaaataaaa cacccactgc attatcgcca attaaatcgt
     1681 gcatattatt attggtgatt ttgatatcat ctcccctgcc ctcacataaa atagcgtaac
     1741 tttcattaca atctgtattg taaaattcgt tgttcgaaat ttcaatatga ttattattgc
     1801 cgtataaatt aattattggc tgataattac cattgtgatt cgtaaatttt aatccgtcga
     1861 ttttaatgta atctttattt tcgatataca tcatcgcctg actgttcccg acagtgctac
     1921 catctaaaat cacaatatcg gtattataat tttgcagggt gatgtaatta cctgcgctgc
     1981 ccccatcgtt ccaggttact ttcccggtat aggtgcctgc catcaggtta atcacatcgc
     2041 catcgttggc catattgagg gcgaattgaa tggttttcca gggagcggct gtggaagtcc
     2101 cggcattggc gttatttccc gaaggtgaaa cataatattc cgttgcagat aattgtaaac
     2161 tgccgaaaag tatacttaag ataaacaggt ttttcatccc ttaaaagtag taattcacag
     2221 gaacaaaaaa aagcccaccc tttcgagtgg actcttgatc atacttggaa ttaatatctt
     2281 ttaatgtata aaccgaatta attcatcaac gctgctgcta aaccaaaact catcgtaaac
     2341 cacaaaataa ttttgtttat ccaccacgtg actatatgca gttttggcta attctagttt
     2401 actgtcttca aaactaaatt cgcgcatcat agtagcaatt tgttctgaag taaaccaacc
     2461 ggattgcact gcttgtctgg ccacattcaa tttagtatca tcaaaccatt gattatggat
     2521 agtttgaatt aacgcattaa atgaagcagc attcatttgt tgctgagcgt tacaaacagg
     2581 ttgttgcggt tgctgatatg gcggataatc aggatatacc ggctgataat aaacaggttc
     2641 atacatttgt aaggcttgaa ctgattttac ataaaatctg ccaaaattat cgataacccc
     2701 atgcactaaa cttgcaggtt tgatatatac ataaccggcg tataagcgca ctcgttgtgt
     2761 ggtagcataa taaccactat gttgaatttt atccgtaaac acttccacat aatgattacc
     2821 cggagccata ttatttacag caaatttttt caccggttca ttaaaaaaat cgccatcaat
     2881 tgcaacggta ataaaattgg cgccttgcga tttaatttcg aaagtagaat tgccataata
     2941 cggatccaca ttcggattag cgggaggaaa agccaataca tgctgcattt ggatgaacac
     3001 cactaacatt gttttaagta aagcattttt cataaaccct ccttttttaa tacgtaaatt
     3061 ccattatcgt gccaaagatg aatgatgagg gatgaatgat gagtgatgag taatgagctt
     3121 ttgatttttc gaaaggctga cgcaattgta tctaatcgta gggacgcatg caatttattt
     3181 aataccctta gcgaatcttt gagtcacttc gctactttgt ggtaaacctt ttgaatttga
     3241 aaataacaat acattttcct tggtagccac gaaggctcca aggggctcga agttgcgcta
     3301 aggtaggcta atgataaaag atgagggatg agtaatgagt ggtgaaggat tggtgtgtaa
     3361 tttttcgaaa ggctggcgca attgtctcta atcgtaagga cgcatgcaat tcgtccctac
     3421 gattagatac aattgctaat ttataattag gcaaaacgat tttgagtggg aattaatttt
     3481 gaattttatg tgtttcggga aatgccgatt ttattttttt gtagggacga attgcatgcg
     3541 tccttacaaa aaaataaaat aggcgaattc gaagacaaaa tgaataagta aagtttgttc
     3601 attcttattc agtatatttt ttaacatcaa aattatagaa tccttaatta cgggtgccga
     3661 accaaatatt accctacttg aaatgtaaac caaaatgcat cttgttggga gcgcttaaag
     3721 ctgtggtttt aacgtattta agttgatggg tatttggtaa tgccaaactg tcgctatgtt
     3781 gaacggtatc gaattgtgca ttacagaggg agactgagat gaatgtgaac aggagggaaa
     3841 atattattga ggaggaagtt tgcatgggat aaaggtaggg ttaaaatgca ggggttgaag
     3901 agtcaattat ttccgatttg gaataggggg aatttgttcg aacgggaaat cctagaataa
     3961 aaatttatct tctattaatg aatcctttaa ttttaaaaaa tgcataaatt aattacaata
     4021 tataatagga ataataagta atgtttatta atctaagcca tcttcttttg tttttattat
     4081 tgtaattaat ttttcattaa tccgggtttt gttgatacca taatgtgctt ctctatgaca
     4141 atttggacaa atagccccca cgttaattgg tatatctgga ccatcgtctg ctaatttatg
     4201 aatatggtga acttctaaaa agggaatacc attggagtca ataaaaggcg ccgtattctg
     4261 gcaatattca caaattccca tagctctttc taacgcatat tttttaattt gcaaacttcg
     4321 aatcctatat tctactatat ttgcaacctt tgtctttact ggtttaacat ctagattttt
     4381 taacttatcc cttaaatgat aaaaagtatc tggttcattt acaatagaaa tatttgaagc
     4441 gtt
//
LOCUS       DAORKW010000285         4412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_930_length_4412_cov_5.425063, whole genome shotgun sequence.
ACCESSION   DAORKW010000285 DAORKW010000000
VERSION     DAORKW010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_930_length_4412_cov_5.425063"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="PLB46_10930"
     CDS             <1..1577
                     /locus_tag="PLB46_10930"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HRF77078.1"
                     /translation="YLQKNKTVTLTSYGEFSAQFPPQFDTQIHENSSWSCVHGVERWR
                     SNCGCSDGGNPGFHQQWRAPLRNALNWLKEKADVVFENEMLPFTPSPWDLRNHYIDIV
                     NNRTESVIQQFFATHFPTKIEEAHKIKMIRLLEMQRHAMLMFTSCGWFFDEVSRIETK
                     QILQYADRVIQIAEHESSSKLYQEFLQLLSLAPSNIAKYATAAGLYETEIRPQRLTLT
                     KVGVHHAILSLFEDMPEVCAVFNYTIKNEFFEKMLAGEHTLTIGNMVINSTFTYAQQD
                     FQYAALYLGQHHVIGGYDETMDSEQLEELFLKLRDAFRKSNLNEVIVLIRNAFPKNTF
                     SFENLFGDAKEQLLNAILAKDIAIAEDNYRAVYEKTFTLVNMLQQQKKHIPELLLRNT
                     AAVINADIKKVFSEVKPNSVKLDQLVDEAIKWKVELDAENIGFVVASRLYDMVDQLAY
                     KVDDLQHLDNLSHLLIKLNELKLKFRLWKIQNKYYRIGKDFIGNEKFMKSLGEQEYKK
                     WLLKFKAVGEQLNIRF"
     gene            1613..4060
                     /locus_tag="PLB46_10935"
     CDS             1613..4060
                     /locus_tag="PLB46_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HRF77079.1"
                     /translation="MFQYADIIQTWYTAKKWHQYPFQHEIAQAYAEGYNGILNAPTGS
                     GKTYAMWIPIVADFMSSKQLLSQPGLKVLWITPLRALAKDICFALQDACDLLDNGWKV
                     ELRTGDVSQKTKQAQKKSMPDCLIITPESLHVLLSGNNYSELFGNLSCVVVDEWHELL
                     GSKRGVQVELALSRLKAIRKNQNNHLRIWGISATIGNLVEAMEVLLGNDATNEKSIVI
                     RSLGKKNMEIHTVLPPSIEVLPWSGHIGLKLLPEIVNIIRASQTTLVFTNTRAMAEIW
                     YHHILNYDENLAGLLAMHHGSLSGEVRDWVEENLHAGTLKAVICTSSLDLGVDFRPVD
                     TVIQIGSPKGVARFTQRAGRSGHQPGAVSKIYFIPTHALELVEISAIKEAVVSNKVEK
                     REPLVMCFDVLMQYLVTLSIGEGFVAEEIYQEIKQTHVFNLIVPEEWNWMIKNIAQGG
                     NTFQAYDEFCKVGKVDGRYRIMNKKAATKHRLQIGTIVSDPVVRIAYLKGGTLGTIEE
                     YYIASLKPGDVFWFAGLCLEFVMLKDMTAFVRKTKEQKAGTASYQGGRMPLSGYMAEL
                     MRKQLDLAAGDNMHNIEFDMLQPLLKLQQQRSVIPKLNQLLVETTESKEGYHIFIYPF
                     EGRVVHEVMSGLAAYRISKLMPVSISVAMNDYGFELLCNQHITVDATLIKNILQTTNM
                     ETDLFNSVNATEMARRKFRDIAVIAGMVFQGYPGELKRARHLQSSSQLIFDVMRMYEP
                     DNLLLKQAYSEILYGQMENARLRSALLRIQNSEILIQQMEKFSPFAFPIVVDSLRDKL
                     TSEKLSDRIQKMVMDNQ"
     gene            4070..>4412
                     /locus_tag="PLB46_10940"
     CDS             4070..>4412
                     /locus_tag="PLB46_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF77080.1"
                     /translation="MEIQHYGQTFMLSPQKCLYWPKHQLLLIADAHFAKETHFRKHGI
                     AVPHGILQHDLLRIEKLIQQFQPKEIIFLGDMFHSEENDGLNEFINWRKKQTLNMTLI
                     IGNHDILPTEWY"
BASE COUNT         1476 a          714 c          892 g         1330 t
ORIGIN      
        1 aatatctgca aaaaaataaa acagtcacgc ttactagtta cggtgagttt tcagcccaat
       61 ttccacctca atttgacact caaattcatg aaaacagtag ttggagctgt gtgcacggtg
      121 ttgaacgctg gagaagtaat tgcggatgtt cagatggcgg aaatccaggt ttccatcaac
      181 aatggagagc tcctttgcgc aatgcattaa actggcttaa agaaaaagca gatgtagtat
      241 ttgaaaacga aatgttgccg tttacaccat caccatggga tttacgcaat cattatattg
      301 atattgtaaa taatagaaca gaatcggtta ttcaacaatt ttttgctacg cattttccga
      361 caaaaattga ggaagcgcat aaaattaaaa tgattcgact tttggaaatg caacgtcatg
      421 ccatgttgat gtttaccagt tgcggatggt tttttgatga agttagccgt atcgaaacaa
      481 aacaaatttt gcaatatgcc gaccgcgtaa ttcaaattgc cgaacatgag tcttcctcta
      541 aattatatca ggaattttta caattgctaa gtctggcacc tagtaatatt gcaaaatatg
      601 ctactgctgc aggattatac gagactgaaa ttcgccctca acgactcaca cttactaaag
      661 ttggtgtaca ccacgccatt ttaagtctgt ttgaagacat gccggaagtg tgcgctgtat
      721 ttaattatac cattaaaaac gagttttttg aaaaaatgtt ggcgggtgaa catacgctta
      781 caataggtaa tatggttatt aattccactt ttacttacgc acaacaagat tttcaatacg
      841 cagccttata ccttggtcag catcatgtta ttggtggcta cgatgaaacc atggacagcg
      901 agcaattaga agaattattt ttgaaattga gagatgcttt tagaaaaagt aatttaaatg
      961 aagtaattgt attgatccgc aacgcatttc caaaaaatac tttttcgttc gaaaatttat
     1021 tcggtgatgc aaaagaacaa ttactcaacg ctattttagc gaaagacatt gccattgctg
     1081 aagataatta ccgtgccgtt tatgaaaaaa catttacact ggttaatatg ttgcagcaac
     1141 aaaagaaaca tattcctgaa ttattgctgc gcaacacagc cgcagttatt aatgccgaca
     1201 ttaaaaaagt gttcagcgaa gtcaaaccga attctgttaa gctcgaccaa ttagtagatg
     1261 aagctattaa atggaaagtt gaactcgatg cggaaaatat cggctttgtt gtcgcttctc
     1321 ggttatatga tatggttgac caactggctt ataaagtaga tgatttgcaa catctcgata
     1381 atttaagtca tttgctgatt aaactcaatg aactaaaatt aaaattcaga ctttggaaaa
     1441 tccagaataa atattaccgg attggtaaag attttattgg taatgaaaag ttcatgaaat
     1501 cgttgggtga gcaggagtat aaaaaatggt tgctcaaatt taaagctgtt ggcgaacaac
     1561 tcaatatccg attctgaaat tttgttgtaa tttactcgat taaatagttg atgtgtttca
     1621 atatgccgat atcatacaaa cctggtacac agctaaaaaa tggcatcaat atccgtttca
     1681 gcacgaaata gcccaggctt atgccgaagg ctataacggc attttaaatg caccaaccgg
     1741 aagtgggaaa acctatgcca tgtggattcc cattgttgcg gattttatgt cctcaaaaca
     1801 attactatca caacccggat taaaagtatt atggattaca ccactgcgtg ctttagctaa
     1861 agatatttgt ttcgcattac aagacgcctg cgatttatta gataatggat ggaaagttga
     1921 actacgcacc ggtgatgttt ctcaaaaaac aaaacaggca caaaaaaaat ccatgcctga
     1981 ttgtttaatt ataacgccgg aaagtttaca tgtgttatta tccggaaata attatagcga
     2041 attatttggc aatttatctt gtgttgtggt tgatgaatgg catgaattgt taggaagtaa
     2101 aagaggtgtg caggttgaat tagcattatc tcggctaaaa gctattcgca aaaatcaaaa
     2161 taatcattta cggatttggg gaattagtgc caccattggc aatttggttg aagcaatgga
     2221 agtgttatta gggaatgatg ccaccaatga aaaatcgatt gttattcggt ctttgggtaa
     2281 aaaaaatatg gaaatccaca ctgtattgcc tccaagtata gaagtattac catggtcagg
     2341 acatattgga ttaaaattgt taccagaaat tgtaaacatt atccgtgcat cacaaactac
     2401 tttagtgttt accaatacca gagcgatggc agaaatctgg tatcaccata ttttaaatta
     2461 cgatgaaaat ttagccggat tattagctat gcatcatggc agtttatccg gtgaagtgcg
     2521 cgattgggta gaagaaaatt tacacgcggg tactttgaaa gcagtaatat gtacttccag
     2581 tttggacttg ggtgtagatt ttcgtccggt tgacacagta attcagattg gttctcccaa
     2641 aggggttgca agatttacac aacgagcggg caggagtgga catcaaccgg gagccgtttc
     2701 taaaatttat tttataccga cacatgcttt agaattggtt gaaatttcag cgataaaaga
     2761 agctgttgta tctaataaag tcgaaaaaag ggaaccatta gtaatgtgtt tcgacgtgtt
     2821 aatgcaatac ttagtaacac tatctatagg tgaaggtttt gttgcagaag aaatttatca
     2881 ggaaataaaa caaacacatg tatttaatct gattgttccg gaagaatgga attggatgat
     2941 taaaaatatt gcacaagggg gaaatacgtt tcaggcgtat gatgaatttt gtaaggtagg
     3001 taaggtggat ggacgctatc ggataatgaa taaaaaagct gcgaccaaac atcgtttgca
     3061 aattggtact attgtgagtg acccggttgt gcgcattgct tatttgaaag gtggaacatt
     3121 aggaacaata gaagaatatt acattgcttc tttaaaaccg ggtgatgtgt tttggtttgc
     3181 aggattatgt ttggaatttg taatgttgaa agatatgaca gcatttgtcc gcaaaaccaa
     3241 agaacaaaaa gcgggcactg catcttatca gggtggtcga atgccactgt caggatatat
     3301 ggctgaatta atgcgcaagc aattagattt agctgccggt gacaacatgc ataatattga
     3361 atttgacatg ttgcaaccat tattaaaatt gcaacagcag cgttctgtaa ttccaaaatt
     3421 gaatcaacta cttgtagaaa ctaccgaaag taaagaaggt tatcatattt ttatttatcc
     3481 atttgaaggt agggttgtgc atgaagtaat gtctggattg gcagcttacc gaataagtaa
     3541 attgatgccg gttagtattt ctgtagccat gaatgattat ggatttgaat tgttatgtaa
     3601 tcaacacata acagttgatg caactttaat taaaaatata ctgcagacca ctaacatgga
     3661 aaccgattta tttaattcgg tgaatgccac agaaatggcc aggcggaaat tccgcgatat
     3721 tgccgttatt gcaggaatgg tatttcaggg atatccgggc gaattaaaac gtgcacgaca
     3781 tctgcagtca tcttcgcaat taatttttga tgtaatgcgt atgtatgaac ctgataattt
     3841 attattgaaa caggcatata gtgaaatttt gtatggacag atggaaaatg cccgattgcg
     3901 gtcagcattg ttacgcattc aaaacagcga aattttaatt cagcaaatgg aaaaattttc
     3961 acctttcgca tttccaattg tggtcgatag tcttcgcgac aagctcactt cagaaaaatt
     4021 aagcgaccgt attcaaaaaa tggtaatgga taatcaataa ctttgagtga tggaaattca
     4081 acactatggg caaacattta tgctatcgcc acaaaaatgt ttgtattggc ctaaacatca
     4141 attgctttta attgctgatg cgcattttgc caaagaaacg catttcagaa agcatggcat
     4201 tgcagttccg catggtattt tacaacatga tttgttgcga atcgaaaaat taattcagca
     4261 attccaacct aaagaaatta tttttctagg cgatatgttc cacagtgaag aaaatgatgg
     4321 attaaatgag ttcattaatt ggcgtaaaaa acaaacactc aacatgacat taattatcgg
     4381 taatcacgat attttaccta ccgaatggta tg
//
LOCUS       DAORKW010000286         4397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_938_length_4397_cov_5.615615, whole genome shotgun sequence.
ACCESSION   DAORKW010000286 DAORKW010000000
VERSION     DAORKW010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_938_length_4397_cov_5.615615"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLB46_10945"
     CDS             <1..326
                     /locus_tag="PLB46_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77081.1"
                     /translation="TKAATGCFKNSNAIAVSVPCREEDLTNNRIINVYPNPASSSVII
                     SINQLTNCNSQLTIADCTGKIISYFDNVSSSSEIDISFYPAGMYFITIQVKDQVETVK
                     FMKIN"
     gene            333..4145
                     /locus_tag="PLB46_10950"
     CDS             333..4145
                     /locus_tag="PLB46_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRF77082.1"
                     /translation="MKPSLLISLIIFNTFKCFGQTPDAESIVDGNQFSANFGNAVSNA
                     GDVNGDGYDDVIVGAYLYDNGQTNEGVAYIFHGSEFGINTSPALILECNQASANFGAS
                     VSTAGDINNDGFDDVIVGANFFDNGQTNEGRAFVYLGSPAGINPIPNATLECDVVGAN
                     FGIAVSNAGDINADGFDDVIVGANLSDHAYVYLGSSDGIDTVEIATLSNFQGNSQFGF
                     SVSDAGDVNADGFDDVIIGAYKFDGEGLNSDGGIAYLYHGNALGLITTPATILEEGFY
                     STYFGYSTSGAGDVNNDGFDDVIVGEYQADFLEFDAGSVFIYLGSASGVNTTFSTRID
                     NEQYFAHFGKAVSSAGDYNADGYVDIVVAASDFDGGSSNEGKVYIYEGSASGIDAIPD
                     ATFESNQASSSFGKSISFAGDVNNDGFSDLLIGANFYDNMFSDEGRIYVFHGNDCPTS
                     TFYPDQDGDGFGSSILSAITCNIPDGYILDNSDCNDLDSLQHPLTIWYFDADEDFYYN
                     DAIPTLTQCSSPGIGYTFNILGGSDCDDNNNQKYPDAEEQPDYYDNDCDGFIDESLNF
                     QFDGFLSIAENGVVDDVSKAGDINGDGFDDIIIGSPDFDTPNMGIAMVFNGSIDGIQL
                     APSFVRSDTQSDSDFGNVAGAGDVNNDGYDDIIIGAPLFDNPSFREGKIEIFYGSSTG
                     LPAIPNLKIEGSMSDLGLGNAVSSAGDINNDGYDDIIVGASLYNSSKGRIYVYYGSAV
                     GITNTPLIINGTLSAQHFGYDVCSAGDVNGDGFDDVAVGAPLSDNGQIDEGRVYIYHG
                     SASGLILSPATILESNQAICQFGFAVAGGGDINNDGFDDLIVGAPNYSNGELYEGSVF
                     VYLGSSTGINSVPVLILENNIQDGRFGNSVSGIQDMDNDNFDDIIIGSPNFSGNFNWE
                     GKIYVYHGNSFALDVDNIETIEINQLEAKFGISVSGAGDLNGDSYGDIVVGAGKYFDG
                     EDIVGGAFVYTSIPCAPSPELCNSLDDNCNGLIDDGITETISITAGGPISFCQGGSVS
                     LTATYSGATVQWKKNGTNIPGATSTTYNVTTKGNYTCVTTSACGTTESTPIFVNVIKN
                     PNASISAGGPTTFCAGGSVVLTEVAVAGCTYQWYKGATPIAGATSLTYTATTSGNYKC
                     RVTKAATGCFKNSNAIAVSVPCREGLPDGEAGEMLIDENACSIYPNPNNGTFTIDANV
                     TTQDFASPISTIEIYNNLGQLIYSEKINSQDGIVNEIVNIGSAVSGIYLLKITHGDYI
                     AQSKIVIE"
     gene            4168..>4397
                     /locus_tag="PLB46_10955"
     CDS             4168..>4397
                     /locus_tag="PLB46_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77083.1"
                     /translation="MKNYIILLLYFILGGEIYSQVPEEEWSKFIGTSGNDDTRSVVST
                     PDGGCLVLGTRSSSFDGDATCFNGLSDIWLFK"
BASE COUNT         1365 a          658 c          952 g         1422 t
ORIGIN      
        1 ttacaaaagc tgcaacaggc tgttttaaaa attcaaatgc aattgcggtg agtgtacctt
       61 gtcgtgaaga agacctcaca aataatagaa taataaatgt ttatccaaat cctgcatcaa
      121 gtagtgtaat tatttcgatt aaccaattaa caaattgtaa ttctcaactt acgattgctg
      181 attgtactgg aaaaataatt agttactttg ataacgtttc atcttcatct gaaatagaca
      241 tttcttttta tccggctgga atgtatttta ttactataca ggtaaaggat caagtagaaa
      301 ctgtaaaatt tatgaaaata aattaaaata atatgaaacc cagtttatta atctcgctta
      361 tcatcttcaa tactttcaaa tgttttggtc aaacacctga tgcagagagt attgtggatg
      421 gaaatcaatt tagtgctaat tttggtaatg ctgtttcaaa tgcgggagat gtaaatggag
      481 atggttatga cgatgtgatt gtcggagcgt atttgtatga taatggtcaa acaaatgaag
      541 gtgtagcata catttttcat ggttcagaat ttggaataaa cacgagtcct gcattgattt
      601 tagaatgtaa tcaagcaagt gcaaattttg gtgcctcggt ttcaacagca ggtgatataa
      661 ataatgatgg atttgacgat gtaattgttg gtgcaaattt tttcgataat ggacaaacaa
      721 atgaaggtag agcgtttgtt tatcttggat cgcccgctgg cataaatcca atcccaaatg
      781 ccacccttga atgtgatgtt gtaggagcaa atttcggcat agctgtttcg aacgcaggcg
      841 atattaatgc tgatggattt gacgatgtca ttgtgggcgc caatttatct gatcatgcat
      901 atgtttatct gggttcgtct gatggaattg atacggttga aattgcaacg ttgtctaatt
      961 ttcaggggaa ttcgcaattt ggattttctg tttcagatgc cggagatgta aatgcagatg
     1021 gatttgatga tgttataatt ggggcttaca aatttgatgg cgaaggttta aatagtgatg
     1081 gcggtattgc ttatttatac cacggaaatg cactaggttt aattacaaca ccagctacaa
     1141 tactcgaaga aggtttttat tcaacatatt ttggttattc aacttcagga gcgggagatg
     1201 ttaataatga cggctttgat gacgtaatag ttggtgaata tcaagcagac tttctagaat
     1261 ttgatgcagg aagtgttttc atttatctgg gcagtgcatc cggagtgaat acaacttttt
     1321 caactagaat tgacaatgaa caatactttg cacattttgg aaaggcagtt tcttcagcag
     1381 gcgattataa cgcagatggt tatgttgata tagtagttgc agcttcagat tttgacggag
     1441 gtagcagtaa tgaaggtaaa gtatatattt atgaaggttc tgcatctgga attgatgcaa
     1501 ttccggatgc tacattcgaa tctaatcaag cctcatcttc ttttggaaaa tcaatttctt
     1561 ttgccggaga tgtaaataat gatggtttta gtgatctcct cataggagca aacttttacg
     1621 ataacatgtt ttcagacgaa ggaagaattt atgtatttca tggaaatgac tgtccaactt
     1681 caacatttta tccagatcaa gatggtgatg gttttggaag ctcaattttg agtgctatta
     1741 cctgtaatat acctgatgga tatattttgg ataattctga ttgtaacgat ttggattctt
     1801 tacagcatcc attaacaatt tggtattttg atgcagatga ggatttttat tataatgatg
     1861 caatacctac tttaacgcaa tgtagctctc ccggcatagg atatactttt aatatacttg
     1921 gaggtagcga ttgtgatgat aataataatc aaaaatatcc tgatgctgag gagcaaccgg
     1981 attattacga taatgattgc gatggattta ttgatgaatc gttaaatttt caatttgatg
     2041 ggttccttag tatcgcagaa aatggagttg ttgatgatgt ttcaaaagct ggtgatataa
     2101 atggtgatgg ttttgatgat attataattg gttcacctga ctttgacaca ccgaatatgg
     2161 gtatagctat ggtatttaat ggctcaattg atgggataca attagcacct tcattcgtaa
     2221 gatccgatac ccaatctgat agtgatttcg gtaatgttgc tggtgcagga gatgtgaata
     2281 atgatgggta tgatgatatt ataattggag cacctttgtt tgataaccca agttttagag
     2341 aagggaaaat cgaaatattt tatggttcaa gtacaggttt acctgcaatt ccaaatttaa
     2401 agatagaggg ttcaatgtct gatttgggtt taggaaatgc agtttccagt gcaggtgata
     2461 ttaataatga tggatacgac gatattatag tcggggcttc gttgtataat agctctaaag
     2521 ggaggattta tgtttattac ggaagtgcag ttggaattac taatactcct ttgataatca
     2581 atggaacttt atcagctcag cattttggat atgatgtctg ctctgcaggt gatgtaaatg
     2641 gagatggatt tgacgatgtt gcagtgggcg ctccattaag tgataacgga caaattgatg
     2701 agggtagagt atatatttac catggttcag catctggact tatcttaagt cccgctacaa
     2761 ttttagaaag taatcaggct atatgtcaat tcggctttgc tgttgcaggt ggcggtgata
     2821 ttaataatga tggatttgat gatttgattg tcggagcacc aaactattca aatggggaac
     2881 tctatgaagg aagtgttttt gtgtatttag gctcatccac aggtataaat tccgtacctg
     2941 tattaatttt agaaaataat atacaggatg gaagattcgg aaattctgtt tctgggattc
     3001 aggatatgga caacgacaat tttgatgata ttataattgg atccccaaat ttttcaggta
     3061 attttaattg ggagggaaaa atatatgttt accatggaaa ttcttttgct ctcgatgtag
     3121 ataacatcga aactattgaa ataaatcaat tagaagcaaa attcggtatt tcagtttcag
     3181 gtgctggaga cttaaatgga gatagttacg gtgacatcgt ggtaggcgcg ggaaaatatt
     3241 ttgatggtga agatatagta ggaggagcat ttgtttatac aagtattcca tgtgcaccat
     3301 cccccgaact ctgcaactcc ctcgacgata actgcaacgg cctcatcgac gatggcatca
     3361 cagaaacaat ttccatcact gctggcggac caatttcttt ttgtcaaggt ggtagcgtgt
     3421 cgctcactgc gacctactca ggtgcaactg tacaatggaa aaaaaatggc accaatatcc
     3481 caggtgcaac aagcacaacg tataatgtta caacaaaagg aaattatact tgtgtaacaa
     3541 ccagtgcatg cggtactaca gaatcaaccc caatttttgt caacgtaatt aaaaacccca
     3601 acgcaagtat ctctgccgga ggcccaacaa ctttttgtgc cggtgggagt gtggtattaa
     3661 ccgaagttgc ggttgcaggt tgtacctatc aatggtataa aggtgcaaca ccaattgcag
     3721 gtgcaacttc attaacgtat acagcaacta cctcaggtaa ttataaatgc agagttacaa
     3781 aagctgcaac aggctgtttt aaaaattcaa atgcaattgc ggtgagtgtg ccgtgcaggg
     3841 aaggattgcc agacggcgag gcaggggaaa tgctgattga tgaaaatgct tgctccattt
     3901 atccaaatcc caacaatgga acattcacaa tcgacgcaaa cgtaacgacg caagattttg
     3961 catcccctat atcaacaatt gagatttata ataaccttgg acaattaatt tattctgaaa
     4021 aaatcaattc acaggatgga atagtcaatg aaatagttaa tatcggaagt gctgttagcg
     4081 gtatttattt gttgaaaatt acacatggtg attatattgc ccaaagtaaa attgtaattg
     4141 aataatttaa tgcattaatt gctgaccatg aaaaattata taatattatt actttacttt
     4201 attctgggcg gcgaaattta ttcgcaagtt cctgaagagg aatggtctaa gtttattgga
     4261 acatcaggca acgatgatac cagatcagtt gtttctactc cggacggtgg atgtctcgta
     4321 ttaggaacta gaagtagtag ttttgatggt gatgctactt gttttaatgg attgtcagat
     4381 atttggctct ttaaatt
//
LOCUS       DAORKW010000287         4392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_940_length_4392_cov_5.466451, whole genome shotgun sequence.
ACCESSION   DAORKW010000287 DAORKW010000000
VERSION     DAORKW010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_940_length_4392_cov_5.466451"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="PLB46_10960"
     CDS             complement(<1..1555)
                     /locus_tag="PLB46_10960"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF77084.1"
                     /translation="MKRTFYLSLLAFLTTFQATAQVVAFSDDFESGLGNWTYTGLWNT
                     TTSESFSPTHCFTDSPDGDYPDVFSSEATLAVDVNLSDALDADIKLMALVDLETGFDY
                     VYLDASTDGGGDWTNIYVFNGEDMFTWTEYDIPLGAFVGSENVRIRFRFESDAAYFVD
                     GLYIDDIQIIKYNIDGAAPLILHTPTNLYEGTLGANTLQAELIDASGIASTTLYYNED
                     GGPYNAVSGINVVGDLYAYEIPELTPGTWVTYYIEATDTYAVPNTATSPDYEIIAGNY
                     IGYDDGIIDFVSDIGTLSLTGYVSAAVKITLTGETDVVTAIIQNYTDYMRPNDDIQFH
                     IWADDGTGLPGDDIIPPFYVTPEPTLDEPNRGTRIDLRGIPELEGIYGDVYIGYTVPG
                     GVAWTSYTNTLVTDRSYVQTAFGWSLLTGDFHFRVVTSELTGAPEVDYTYSAVAEPLI
                     SFTDLSTNDPTSWFWDFGDGGTSTEENPSHNYLSNGSFNVCLTATNAIGSGTHCEFVV
                     IDNYLTPVTD"
     gene            1696..2505
                     /locus_tag="PLB46_10965"
     CDS             1696..2505
                     /locus_tag="PLB46_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF77085.1"
                     /translation="MKKWLFIVFVINSVVTQAQTADQWFESAYDKYENHDYAGAISDY
                     TECIMLTPEFPEAYFNRGLSNFFAGFYDAAIFDFNQVVMLKPGEAKAYYWKGFTYGEQ
                     GKLEDAENAYTKALYYDPYYVEAFFARGNIRYSLGKLDESIEDFNKVISLQNNFADAV
                     YNRGLSFLANKNYTSALQDFSKVVELDQEDADAWYYIGFIYNQTNNYEQAVIALTKVI
                     GMESDAGDAYFERGIAWFNLMKDEDACTDWSEGILIGNEMCKKMYDAECVQ"
     gene            complement(2507..3106)
                     /locus_tag="PLB46_10970"
     CDS             complement(2507..3106)
                     /locus_tag="PLB46_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF77086.1"
                     /translation="MKSLTLSICILLSISTFAQPGEQITVSAQQDSIVFTVNGETSAI
                     ANKDMAAYYLSEGIAAAIEGDYTGAESKFKVGLLYDLENAEILYNLGLAQYYLEKFEE
                     AIKTFDAAADYDPENKDIYNQRGLCKARSGLLQEAEMDFIIMLKYDPTFAMGNYNYGI
                     LKLQMGEQEMACELLNKADQYGYENAPAVIATYCNTFDN"
     gene            complement(3304..>4392)
                     /locus_tag="PLB46_10975"
     CDS             complement(3304..>4392)
                     /locus_tag="PLB46_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRF77087.1"
                     /translation="GTDHAIVDVAEAKAVAAKAGYPILIKASAGGGGKGMRVVNNDEE
                     LEEQMHRAMSEALSAFGSDAVFIEKFVSSPRHIEIQVLADIQGNVVYLFERECSIQRR
                     HQKLVEEAPSSVVSPEMRKAMGESAVNAAKACNYRGAGTVEFLVDADLNYYFLEMNTR
                     LQVEHPVTEFITGLDLVREQILIAEGKPLSFTQEDLKIHGHAIELRVTAEDPTNNFLP
                     DIGKLITYRRPQGHGIRVDDGYEEGMDIPIYYDPLLSKLIVHAATRELACKKMIRAID
                     DYKISGVATTLPFGKYVMQHHAFLSGQFDTKFIETYFTPDQLKSENAGEEKIAALMAI
                     KVLEENGSAAIATHTVNEERSKWKINRL"
BASE COUNT         1375 a          861 c          764 g         1392 t
ORIGIN      
        1 aatcagtaac cggagttaaa taattatcaa ttacaacaaa ttcacaatgt gttccgctgc
       61 caattgcatt ggttgcagta agacatacat taaatgaacc gttgcttaaa taattatgtg
      121 atggattttc ttctgtgctt gtaccgccat caccaaaatc ccaaaaccat gaagtagggt
      181 cattcgtaga taaatcggta aaactaatta atggctctgc aactgctgaa taagtataat
      241 caacttcagg tgcacctgtt aattcagatg ttactactct aaaatgaaaa tcaccggtta
      301 ataacgacca gccgaaagct gtttgcacat aactgcggtc agtcaccaag gtgttcgtat
      361 aagatgtcca agctacgcca ccgggaacag tatagccgat atatacatca ccataaatgc
      421 cttctaattc cggtatacca cgcaagtcaa tgcgtgttcc tcgattaggt tcatctaatg
      481 ttggttcagg tgtaacataa aaaggtggaa tgatatcatc acctggtaat cctgtgccat
      541 catctgccca aatatgaaat tgtatatcat catttggtcg catataatcg gtataatttt
      601 gaataatcgc tgttactaca tctgtttctc ctgttaatgt aattttcact gcagctgaaa
      661 cataacctgt taaacttaat gtaccaatat cagatacaaa atcaattatt ccatcatcat
      721 aaccaatata atttcctgca attatttcat aatcaggtga agttgctgta tttggaacgg
      781 cataggtatc ggttgcttca atataatacg ttacccaggt gcctggagtt aattcaggaa
      841 tttcataagc atataaatcg ccaacaacat taattcctga aacggcatta taaggtccgc
      901 catcttcatt ataatacaaa gtggtggatg cgatacccga agcatcaatt aattctgcct
      961 gcaaagtatt tgcacctaat gtgccttcat ataaattagt tggtgtgtgt aaaattaaag
     1021 gtgcggcacc atcaatatta tatttgataa tttgtatgtc atcaatatat agcccatcaa
     1081 caaaatatgc agcatcactt tcaaaacgaa aacgaatacg cacattttcg cttccaacaa
     1141 atgctcctaa cggaatatca tattctgtcc aggtaaacat gtcttcacca ttaaatacat
     1201 aaatatttgt ccaatcgcca ccaccatctg tagatgcatc caaatacaca taatcgaagc
     1261 cggtttccaa atctactaag gccattagtt taatgtcggc atctaaagca tccgataaat
     1321 ttacatcaac agccaaagtt gcttctgaac tgaatacatc cggataatca ccgtcgggac
     1381 tatcggtaaa acaatgtgta ggactgaacg actcgctggt agtggtattc cacaatccgg
     1441 tgtaagtcca gtttccaagt ccgctttcaa aatcgtcact aaaagctaca acctgcgccg
     1501 tagcctgaaa tgtcgttaaa aaagcgagga gagacaagta aaaagttctt ttcatgattt
     1561 gtttaatttg gttaatatca tctaaattaa aacaatttta tgattttggc agacatttcg
     1621 cagtaaaagt ttcgccttgg caaacgcaat cggggaaatt gtggttaact ttgcccaaaa
     1681 attggcacaa ccggcatgaa gaaatggcta tttattgttt ttgttattaa ttcggtagtt
     1741 acgcaggctc aaactgccga tcaatggttc gagtcggctt acgacaaata tgaaaatcac
     1801 gattatgcag gtgcaatatc cgattatacg gaatgtatta tgcttacgcc ggaatttcct
     1861 gaagcctatt ttaaccgcgg tttaagcaat ttttttgcgg gattttacga tgcggctatt
     1921 tttgatttta atcaggtagt aatgttaaaa cccggtgaag caaaagccta ttattggaaa
     1981 ggttttactt acggcgaaca gggtaaatta gaagatgctg aaaatgccta tacaaaagca
     2041 ttgtattacg acccctatta tgttgaggca ttttttgccc gtggtaatat tcgatacagc
     2101 ttaggaaaat tagatgaatc aatagaagat tttaacaaag taatcagcct ccaaaataat
     2161 tttgccgatg ctgtttataa cagaggatta agttttctgg cgaataaaaa ttatacatct
     2221 gccttacaag atttttcgaa agtggtggaa ttagaccagg aagatgctga tgcctggtat
     2281 tatatcggat ttatttacaa ccagaccaac aactacgaac aagctgttat tgcacttact
     2341 aaagtaattg gaatggaatc agatgccggt gacgcttatt ttgaacgtgg cattgcatgg
     2401 tttaatttaa tgaaagatga agatgcctgc accgattgga gtgaaggaat tttaatcgga
     2461 aatgagatgt gcaaaaagat gtacgacgct gagtgtgtac aataaattaa ttgtcaaaag
     2521 tattacaata agtagcaatt actgcaggcg cattttcata gccgtattgg tctgctttat
     2581 ttaacaattc gcaagccatt tcctgttcgc ccatttgtaa ttttaaaata ccataattat
     2641 aattacccat tgcaaaagtc gggtcgtatt tcaacataat aataaaatcc atttctgctt
     2701 cttgtaataa gcctgaacgc gccttacata atccacgctg attataaatg tctttatttt
     2761 cagggtcgta atcggcagct gcatcaaaag ttttaatggc ttcctcaaat ttttctaaat
     2821 aatattgcgc taatcctaaa ttgtataata tttctgcatt ttctaagtca tacaacaagc
     2881 caactttgaa tttactttct gcacctgtat aatctccttc aattgcagct gctattccct
     2941 ctgacaaata ataggcagcc atatccttat tggcaatagc acttgtttcg ccatttaccg
     3001 taaaaactat actatcctgc tgagcagaaa ccgtgatttg ttcaccgggt tgagcgaaag
     3061 tgctgatact cagcagtatg catattgaca gggttaaact tttcatttat tttgcttttg
     3121 gtggaaacaa ataaacggat tttcccggaa attatttgat tttgacttcg atttaggaaa
     3181 taagttactg tgaaaattat gagcagcagg aataaagtgc tcaacttaat ttaaatgcac
     3241 agcacctaat tacaattgca tgtaaacgaa tatactgaca atataattcg ataaaatgga
     3301 atattacaag cggttaattt tccacttgct tctttcttca ttcacagtat gagttgcaat
     3361 tgctgcggag ccattttctt caagtacttt aattgccatt aatgcggcta ttttttcttc
     3421 cccagcattt tcacttttta attgatcagg agtgaaatac gtttcaataa attttgtgtc
     3481 gaattgtccc gataaaaatg catggtgttg catgacatat ttaccaaatg gtaaagtagt
     3541 agctacgcct gaaattttat aatcatcaat tgcacgaatc atttttttgc aagccaattc
     3601 tcttgtagca gcatgcacaa ttaatttaga taaaagtggg tcgtaataaa ttggaatatc
     3661 cattccttct tcataaccat catccactct gataccatga ccttgtggtc ggcgataggt
     3721 gatgagtttt ccaatatccg gtaaaaaatt attggttgga tcttccgcag ttacgcgtaa
     3781 ttcgatggca tgaccatgaa tttttaaatc ttcctgtgta aacgaaagcg gtttaccttc
     3841 agcaattaat atttgttcgc gtactaaatc caaaccggta ataaattctg taaccgggtg
     3901 ttctacttgt aaacgcgtat tcatttcgag gaaataataa ttcagatcgg catccactaa
     3961 aaattcaaca gttcctgcac cacgataatt acatgcttta gctgcattta ctgcgctttc
     4021 gcccattgct tttcgcatct caggactcac tactgatgat ggtgcttctt ctactaattt
     4081 ttgatgacgg cgctgaatac tacattctct ttcaaataaa tacaccacat tgccttgtat
     4141 atctgctaat acctgaattt ctatatgtct cggagaggaa acaaattttt caataaacac
     4201 tgcatcactg ccaaatgcag aaagtgcttc actcatcgca cgatgcattt gttcttcgag
     4261 ttcttcatca ttattcacta cacgcattcc tttaccacca ccacctgcgc tagctttgat
     4321 taatatcgga taaccggctt ttgctgcaac agctttagct tcggcaacat ccacaatagc
     4381 atggtcggtt cc
//
LOCUS       DAORKW010000288         4369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_944_length_4369_cov_3.759388, whole genome shotgun sequence.
ACCESSION   DAORKW010000288 DAORKW010000000
VERSION     DAORKW010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_944_length_4369_cov_3.759388"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..595)
                     /locus_tag="PLB46_10980"
     CDS             complement(59..595)
                     /locus_tag="PLB46_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77088.1"
                     /translation="MENILFLVIGLVVGFIAAWIISKLVAGKNMVNRNELTTLQNELM
                     QANSGNKFLQEKINDIQKTASETEFKLNTVTQEKETANITLSSVKTTLDKLDIDYKLV
                     KQQFDELQNQHNELKDYIIKLRGENDVLKEKNNTIKKEIEDLGEKYTTQFKNLASEIL
                     EDKSKRFTEENQKNIRLI"
     gene            complement(672..745)
                     /locus_tag="PLB46_10985"
     tRNA            complement(672..745)
                     /locus_tag="PLB46_10985"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(709..711),aa:Met,seq:cat)
     gene            complement(806..1660)
                     /locus_tag="PLB46_10990"
     CDS             complement(806..1660)
                     /locus_tag="PLB46_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF77089.1"
                     /translation="MQLYSINAGLFKLDGGAMFGVVPKKIWNKLVPADENNLCTWAMR
                     CMLAVGNGKVVLIDCGMGDKQDAKFFGHYEPHGDFSIEASLKQLGMVPGDVTDVFLTH
                     LHFDHCGGAVKRTDKGKLIPTYENARYWSNERQWNWANNPNAREKASFLTENFVPLQQ
                     HGVLHFIEEHEELFPGFSTMWTYGHTEAMMLPVIKYNGSKVVYCADLLPSHAHIPLPY
                     VMGYDVRPLITIKEKESFLKEACDKKYFLFFEHDSQHECCSLEKTDKGIRPKNFYQLS
                     ELPVNALF"
     gene            complement(1758..2525)
                     /locus_tag="PLB46_10995"
     CDS             complement(1758..2525)
                     /locus_tag="PLB46_10995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRF77090.1"
                     /translation="MKKLFWKLFNAIDERLKFPALQKGETAIQLGFDMSAPVTSDLFL
                     LVNRVGKNGLVIGIDPDPRNVAAAHLIVERQKYPIQLIHKAVFNTSGTMELILGEKAS
                     WNQLKNVPLDTTVSYNGNEVVVEMDTLDNIISQAQIDIATIGHINVTINGAEYFALQG
                     MHQILSQSKNLSLTVVAGRYDDSGTINGQPDFKVIKQLLQQYGFTTRFRRIHKLIWWG
                     FIVNTLMKRKWIYGQKNYGIIMAAKGNKSIPWYQSFS"
     gene            complement(2544..3035)
                     /gene="ribH"
                     /locus_tag="PLB46_11000"
     CDS             complement(2544..3035)
                     /gene="ribH"
                     /locus_tag="PLB46_11000"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRF77091.1"
                     /translation="MASKHQHLSLYREETVPNGDSFRLGIVVSEWNTDITEKLYQGCY
                     NTLLKHKAKSENIISVYVPGSFELPQAAQLLLESQELDAVICLGCIIKGETSHDLHIA
                     SAVAHGIMNLGIDYSTPVIFGVLTTENMQQAVDRAGGKYGNKGDEAAVTALKMAALRE
                     KLI"
     gene            complement(3035..3727)
                     /locus_tag="PLB46_11005"
     CDS             complement(3035..3727)
                     /locus_tag="PLB46_11005"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF77092.1"
                     /translation="MSEEQKPLVDAQVEEQGSYKNVEDFFLKNRNMIIGALGVLILAI
                     GGYFGYKKLYMEPRVITAQDKIAGAEAYFNKDSFALALNGDGAVSGFLAVIENYGNTP
                     SGNMAHYYAGICYLKAGDLDNTIKHLEDYKPGTDELAGLTYVTLGHAYADKKDYKKAI
                     ELYKKAGESADNNLQSPTYYKYAGDLMAEQNDLKGALDMYRKIKQLYPLSQEGQNIDL
                     DITYAETKLGGK"
     gene            complement(3745..>4369)
                     /locus_tag="PLB46_11010"
     CDS             complement(3745..>4369)
                     /locus_tag="PLB46_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRF77093.1"
                     /translation="MGAGIGFAEKYKGTGNVCICYFGDGAVRQGSLHETFNMAMLWKI
                     PVVFICENNGYAMGTSLERTTNQMDMYKIGLAYDMPSYPVDGMQPETVHAAVYEAVEK
                     ARNNEGPTFLEIRTYRYRGHSMSDPAKYRTKEEVEEYKMRDPLETTMQKILDKKYATM
                     KEIEDIQERIEVEIEACVKFAEESPYPDDDAVFEDIYVQQDYPFIKD"
BASE COUNT         1270 a          867 c          757 g         1475 t
ORIGIN      
        1 tttcgttggt tgcttctact ttcattttga attctgttaa atcaacacct aattggtctt
       61 aaattaatcg aatatttttc tgattttctt ctgtaaatct tttgctttta tcttctaaaa
      121 tttctgaagc taaattttta aattgggtag tatatttttc acctaaatct tcaatttctt
      181 ttttgatggt attatttttt tcttttaata catcattttc accgcgcaat ttgatgatat
      241 aatcttttaa ttcattgtgt tgattttgta attcgtcaaa ttgttgtttt accaatttgt
      301 aatcaatatc aagtttatct aatgttgttt ttacactgga taaggtgatg ttagctgttt
      361 ctttttcctg cgttactgta ttgagtttga attcggtttc acttgctgtt ttttgtatat
      421 cgttgatttt ttcctgtaaa aatttattac ctgaattggc ttgcattaat tcattttgta
      481 atgtagttaa ttcatttctg ttgaccatgt tttttcctgc aacaagcttg ctgattatcc
      541 aggcagctat aaatcctaca actaaaccaa ttactaaaaa aaggatattt tccatattgt
      601 aaattaactg aactaaacgg taaaattaaa gtgaatacca aaaaaaaaag caaccttgtt
      661 ggttgcttta gtagcgtggg ggaggctcga actctcgacc tcagcattat gagtgctgcg
      721 ctctagccag ctgagctacc acgccattgg gcggcaaaga taagcactat gcttttgaaa
      781 ataaaataag ttgataaaaa aagcttcaaa aaagcgcgtt aaccggaagt tcactaagct
      841 gatagaagtt tttgggacgt atacctttgt ccgttttttc gagactgcaa cattcatgct
      901 gactatcgtg ttcaaaaaac aggaaatact ttttgtcgca agcctctttc aaaaacgatt
      961 ccttttcttt tatggtaatt aaaggacgca catcgtagcc cattacgtag ggtaggggaa
     1021 tatgcgcatg acttggcagg aggtcggcac aatacactac tttactgcca ttgtatttga
     1081 ttacaggcaa catcatagct tcggtatgtc cataggtcca catggtactg aaccccggaa
     1141 ataactcctc atgttcttct ataaagtgta atacaccgtg ttgttgcagt gggacaaaat
     1201 tttcggttag gaacgatgct ttttctcttg cattcggatt atttgcccag ttccattgcc
     1261 tttcgttaga ccagtaacgg gcattttcgt aggtcggtat caatttgcct ttgtcggtac
     1321 gtttaaccgc accaccacaa tggtcaaaat gtaaatgagt taaaaaaaca tccgtaacat
     1381 caccaggcac catgcccaat tgtttgagag aagcttcaat actaaaatcg ccatgtggtt
     1441 catagtggcc gaaaaacttc gcatcttgtt tatcgcccat gccacaatca atcaacacca
     1501 ctttgccatt gccaacagcc aacatacaac gcatagccca tgtgcagagg ttgttttcat
     1561 cagcaggaac taatttgttc catatttttt tgggcacaac accaaacatg gcaccaccat
     1621 ctaatttaaa taggccggca ttaatcgaat acaattgcat acggctaaag taataattat
     1681 tgtaatataa taggtaattg gagaaagaaa ttgctggttg agatggcatt ctgcggccaa
     1741 atcaaaacat gtagccatta actaaacgat tgataccagg gaatggattt gttgccttta
     1801 gcagccatga taatgccgta atttttttgg ccataaatcc atttgcgttt catgagtgtg
     1861 ttcacaataa aaccccacca aattaattta tgtattcgac gaaaacgtgt tgtaaatccg
     1921 tattgctgca ataattgttt aatcacttta aaatccggtt gaccgttaat agttcctgaa
     1981 tcatcatatc ttcctgcaac aactgtcagc gataaatttt tcgattgcga taaaatttga
     2041 tgcataccct gtagtgcaaa atattccgca ccatttatag ttacgttaat atggccaata
     2101 gtagcaatgt cgatttgtgc ttgagaaata atattatcca gcgtgtccat ctccacaaca
     2161 acttcattac cattatagga caccgtagta tctaaaggga cattttttaa ttgattccag
     2221 ctcgcctttt cacccaaaat taattccata gtgccggatg tattaaatac cgctttatga
     2281 attaattgaa tcggatattt ttgacgctcc acaattaaat gagctgcagc aacatttctc
     2341 gggtcagggt caataccaat tactaatccg ttttttccaa ccctgtttac caataaaaat
     2401 aaatcggagg taacgggagc gctcatatcg aaacctaatt gaatggcagt ttcacctttt
     2461 tgtaaagcag gaaattttaa tcgctcatcg atagcgttaa atagtttcca aaataatttt
     2521 ttcataagtg aaaggctatg cgattaaatt aatttttctc gcaaagcagc cattttaagt
     2581 gcagtaactg ccgcttcatc gcctttgttg ccatatttac cacctgcgcg atcgacagcc
     2641 tgttgcatat tttcggtggt gagtacacca aaaattaccg gagtagaata atcaatgcct
     2701 aaattcataa tgccatgtgc aactgcagaa gcaatatgta aatcgtgtga tgtttcacct
     2761 ttaatgatac atcctaaaca aataaccgca tctaattcct gcgattctaa taatagttgc
     2821 gctgcttgtg gtaattcaaa actgccgggc acataaacac tgataatatt ttcagatttt
     2881 gccttgtgtt tgagcaaggt attgtaacaa ccctgataca gtttttcggt aatatcagtg
     2941 ttccattccg acacaacaat gccgagtcgg aatgaatcac cgttgggtac agtttcttcc
     3001 ctatacaatg aaagatgctg atgttttgaa gccattattt cccaccgagt tttgtttcgg
     3061 cataggtaat atcaagatca atattctgac cttcctgaga aagcggatat aattgtttga
     3121 ttttacgata catatctaat gcacctttta aatcattttg ctcagccatt aaatcgccgg
     3181 catatttata ataagttggg ctttgtaaat tgttatcagc tgattcgccc gcttttttat
     3241 ataattcgat agcttttttg taatcttttt tgtccgcata tgcatgacct aaagtaacat
     3301 atgtaagacc agctaactca tcagtgcccg gtttgtaatc ttcaagatgt ttgatggtat
     3361 tatctaaatc acctgctttt aaataacata taccggcata ataatgtgcc atattgcctg
     3421 aaggtgtatt gccgtaattt tcaattaccg ctaaaaaacc tgaaacagcg ccatcgccat
     3481 ttaatgcgag tgcaaatgag tctttgttaa aataagcttc ggctccggca attttgtcct
     3541 gagcagttat aacacgcggt tccatgtata attttttgta accgaaataa ccgccaattg
     3601 ctaaaataag tacacctagt gcaccaataa tcatattgcg atttttgagg aaaaaatctt
     3661 ctacattttt atatgaacct tgttcctcta cctgagcgtc aactaatggt ttttgttctt
     3721 ctgacataat agttattgtt gaaattaatc tttaataaat gggtaatctt gttgcacata
     3781 aatatcttca aatacagcat cgtcatccgg atatggtgac tcttccgcaa atttcacaca
     3841 tgcttcaatt tctacttcga tacgctcctg aatgtcttca atttctttca tggtagcata
     3901 ttttttgtcg agtattttct gcatggttgt ttccagcgga tcgcgcattt tgtattcttc
     3961 cacttcttct ttggtgcgat atttcgcagg gtcgctcatg gaatgaccgc gataacgata
     4021 agttctaatt tcaaggaaag taggaccttc attattacgt gctttttcta cggcttcata
     4081 aactgctgca tgcaccgttt caggttgcat accatcaaca gggtaagaag gcatatcgta
     4141 agctaaaccg attttataca tgtccatttg gttggtggtg cgctccagcg aagtgcccat
     4201 tgcgtatcca ttgttttcgc aaatgaatac caccggaatt ttccacaaca tagccatgtt
     4261 aaaggtttcg tgtaatgagc cctgacgaac agcgccatca ccgaaataac aaatacaaac
     4321 attacctgtg cctttatatt tttcggcaaa cccaataccg gcgcccatt
//
LOCUS       DAORKW010000289         4367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_948_length_4367_cov_5.060993, whole genome shotgun sequence.
ACCESSION   DAORKW010000289 DAORKW010000000
VERSION     DAORKW010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_948_length_4367_cov_5.060993"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PLB46_11015"
     CDS             <1..981
                     /locus_tag="PLB46_11015"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRF77094.1"
                     /translation="EENQKNIKLILDPLNNDIKEFKKKVEETYEKENKQRFSLEEKIK
                     DLVSLNKLISEEANNLTKALKGQRKTQGDWGEMILENILQQSGLTEGREYVKQEFLRD
                     EAGNTLFNDDNQKMQPDFVIMYPDKREIIIDSKVSLSAYERYSSSEDEEAQKSELNLH
                     LDAMYKHIDELHRRNYQKFAKTLDFVMMFIPVEPAYMLAMQKDNDIWQYAYRKKVLLI
                     SPTNLIAAVKLVAELWKKEEQNKNAIAIADRGAALYDKFVGFVENLQAIGDSINKSQR
                     SYDEAMKQLKDGSGNLIGQAEKLRRLGVQGKKSLPGTVLNDADIDDELIN"
     gene            complement(974..1945)
                     /locus_tag="PLB46_11020"
     CDS             complement(974..1945)
                     /locus_tag="PLB46_11020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HRF77095.1"
                     /translation="MSQFRTIVHVDPLPKPLLVESKVYFAGSCFAENIATKMQQLRME
                     ILVNPFGIIYNPIALAEMLHMQKTNTQIQVSDIFSANGVYQNWHFHSKLGDVNPETAL
                     RNMQQVVWQGHQFLQTANYLIITFGTAFVYELPDGKIVANNHKMAAGNFKKRLLSFEE
                     ILYAWNAVITELKSINPNLIVIFTISPVRHLRDGVVENNLSKATLIQAVHKIIAANAM
                     CYYFPAYELMMDDLRDYRFYAADMVHPNQVAIDYIWERFVSAYTSKDFGDFQLAMEVL
                     NAAKAHQPFHPNSEAHLLFKQQQLEKIKKFQITWPHIPMDDLMQHFS"
     gene            2044..2460
                     /locus_tag="PLB46_11025"
     CDS             2044..2460
                     /locus_tag="PLB46_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77096.1"
                     /translation="MIVAQMDHRIHIIIHSDTKSSLAEIMSGYLQFYSNNTIDITLKT
                     EAPVIHPLAEQVLKEDGIEYKKPTKPTGKSNSTHNIYINFPDTKAIAGKNAKQFNFED
                     PLVLNDYEKVLTAFRATREAIKKVSIELVGEIAVAK"
     gene            2777..>4367
                     /locus_tag="PLB46_11030"
     CDS             2777..>4367
                     /locus_tag="PLB46_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HRF77097.1"
                     /translation="MKKILLSCLAVALGFTSVKAQVPVTVSGEITTNTTWTKNNIYLL
                     NGFVYVEDGATLTIEAGTLIKGDKATKGTLIVTKTGTIQAAGTECEPIVFTSNEPAGT
                     RNYGDWGGIIILGEAPINVAGGSAVIEGGVDTPEGDAAYGGANPADNSGTLQYVRIEF
                     PGIPFLPGNEINGLTLGGVGSGTTIDHIQVSYSGDDSFEWFGGRVNTKYLIAYRGFDD
                     DFDTDFGYRGKNQFGYALRDPNVADVSGSNGFEQDNDATGSANSPYSKALFSNYTIAG
                     PKVNTGDVVNINYKRGAHDRRNTQASIYNSIIMGYPETGLKIEGTNTSQNAMNDSLQF
                     KNNILSGNVDDYICTTCDVGFNIDTWAASNSNQTYLTNNLVGLVDVFNLSNPDPRPTA
                     GSAPTTIGTYFSGLLSDPFFTPTSYTGAFSTSDNWTDESWVNFDPQNTPYTTAGINNN
                     PVVTATVTNASCAGTGAIDVSVTGGAGGYTYSWSDGPTTQDRSFLNAGTYTVTVTSNG
                     CSVVKSYTVNDVTVAKPTSLAI"
BASE COUNT         1470 a          777 c          805 g         1315 t
ORIGIN      
        1 gaagagaacc agaaaaatat taaactcatt ttagatccgc tcaataatga tatcaaagaa
       61 ttcaaaaaga aagtagaaga aacctacgaa aaagaaaaca aacaacgttt ttcacttgaa
      121 gaaaaaatta aagacctggt tagtttaaat aaactcatta gcgaagaagc caataatctt
      181 actaaagcat taaaaggtca gcgcaaaacc cagggcgact ggggtgaaat gattttagaa
      241 aacattttac aacaatcggg acttaccgaa ggccgcgaat atgtgaaaca ggaattcctg
      301 cgcgatgaag cggggaacac cttattcaat gatgataatc aaaaaatgca acctgatttt
      361 gtcattatgt atcccgacaa aagagaaata attattgaca gcaaagtatc attaagtgct
      421 tatgaaagat atagttcatc agaagatgag gaagcgcaaa agtcggagtt gaatttgcat
      481 ttagatgcca tgtataaaca tattgatgaa ctgcacagaa ggaattacca gaagtttgcc
      541 aaaacattgg attttgtcat gatgtttatt cccgttgaac cggcatacat gctggccatg
      601 caaaaagata atgatatttg gcagtacgcc tatcgcaaaa aagtattatt aatttctcca
      661 accaatttaa ttgctgcagt aaaattagta gccgaattat ggaaaaaaga agaacagaat
      721 aaaaatgcca ttgctattgc cgacagaggt gcagcattgt acgataaatt tgttggtttt
      781 gtagaaaatt tacaggctat tggcgatagt ataaataaat cgcaaaggag ttatgatgaa
      841 gccatgaaac agctcaaaga tggctcgggt aatttaattg gtcaggcaga aaaattacgc
      901 agacttggcg tgcagggtaa aaagagttta ccgggtaccg ttctcaacga tgctgacatt
      961 gatgatgaac taatcaactg aaatgttgca tcaaatcatc cattggaata tgcggccagg
     1021 ttatttgaaa ttttttaatt ttttctaatt gttgttgttt aaataacaaa tgtgcttctg
     1081 aattcggatg aaatggctgg tgcgcttttg ctgcgtttaa tacttccata gctaattgga
     1141 aatccccaaa atcttttgaa gtataggcac tgacaaatct ttcccagata taatctattg
     1201 ccacctggtt ggggtgtacc atatcggcgg cataaaaacg ataatcgcgc aagtcatcca
     1261 tcatcaattc gtaagccggg aaataataac acattgcatt tgcagcaatt attttgtgga
     1321 ctgcttgtat taaagttgct ttacttaaat tattctccac aaccccatcg cgcaaatgcc
     1381 gcaccggact tattgtaaat attacaataa gattaggatt aatcgatttg agttccgtta
     1441 taacggcatt ccaggcatac aatatttcct caaacgaaag caatcgtttt ttaaaattcc
     1501 cggctgccat tttatgatta ttggcaacaa ttttcccatc gggtaattca tatacaaagg
     1561 cagtcccgaa agtaatgatt aaataattgg cagtttgcaa aaattggtgg ccttgccaaa
     1621 cgacttgttg catattgcgt aatgccgttt cgggattgac atctccaagc ttactatgaa
     1681 aatgccagtt ttggtaaaca ccgtttgcag aaaagatatc agatacctgt atttgtgtat
     1741 tcgtcttttg catgtgcagc atttcagcta acgcaatggg attgtaaata atgccgaacg
     1801 gatttactaa tatttccatc cgcaattgtt gcatttttgt tgcgatattt tccgcaaaac
     1861 aggaaccggc aaaatacact ttactttcca ccagcaacgg tttaggtaat gggtcaacat
     1921 gcacaattgt ccgaaactga ctcatattta atattacttt ttaagaataa gtttgcaatt
     1981 tactgttata attttatttt tacatgcaca ttcgtgcacc tatattaaaa caatgttaat
     2041 ttaatgattg ttgctcaaat ggaccaccgt attcatatca ttatacatag tgatacaaag
     2101 tcaagcctgg ctgagatcat gagcggttat ttgcaatttt acagcaacaa taccatcgac
     2161 attaccctta aaactgaagc acctgtcatc catcccttag ccgaacaggt tttaaaagaa
     2221 gatggcattg aatacaaaaa accgacaaaa ccgacaggta aatccaattc tacacataat
     2281 atctatatta attttcctga taccaaagcc atagcaggaa aaaacgcaaa gcaatttaat
     2341 ttcgaagacc cactggtatt aaacgactat gaaaaagtgt tgacagcgtt tcgtgcaaca
     2401 cgagaggcta ttaaaaaagt aagtatcgaa ttggttgggg aaattgcagt agctaaataa
     2461 ttacactcct aatcttccat gtaaattaat tgttactact tcaattaagt gttaatactg
     2521 gttgaattta attttctcat ttacgattgc tgcattttag gcacatttct agacattgaa
     2581 taaaattgtt catataattc tgcttttaat tttaactgga attaacaata aataagcaaa
     2641 gttgaatttt cctaacacac aatttacata ttagccgcta tttcatcatt ttattataac
     2701 gctctgtcaa tcagtgctta atacaacggt tctaggtttg cactttaata ttttctaacg
     2761 ctaaatgcaa tcaacaatga aaaaaatttt actgagctgt cttgccgtcg cactgggatt
     2821 tacgtcggtt aaagcacaag tacccgttac tgttagcggc gaaattacca cgaataccac
     2881 gtggacaaaa aacaacatct atttattaaa tggatttgtt tatgtagaag atggtgcaac
     2941 attaaccatc gaagctggaa ccttaattaa aggtgacaaa gcaacaaaag gtacattaat
     3001 tgttaccaaa acaggaacta ttcaggcagc aggtactgaa tgtgaaccta tcgtatttac
     3061 atctaacgaa cctgcaggta caagaaatta cggtgactgg ggtggtatca tcattttagg
     3121 tgaagcacca attaacgttg ccggtggttc agcagttatt gaaggtggtg ttgacacacc
     3181 ggaaggtgat gctgcttatg gtggagcaaa tccggcagat aacagcggca cattacaata
     3241 tgtgcgtatc gaattccctg gtattccttt tttacctggt aatgaaatta acggtttaac
     3301 attaggtggt gttggtagcg gaactactat cgaccatatc caggtttctt attcaggtga
     3361 tgattcattt gaatggtttg gtggtcgcgt taataccaaa tacttaattg cataccgtgg
     3421 atttgatgat gattttgata cagactttgg atacagaggt aaaaatcaat ttggttatgc
     3481 tttacgtgac cctaacgtag ctgacgtttc aggttcaaac ggatttgaac aagataatga
     3541 tgctactggt tctgccaatt caccttattc taaagcatta ttttccaact acactattgc
     3601 cggaccaaaa gtaaataccg gtgatgttgt aaacattaac tacaaaagag gagctcacga
     3661 cagaagaaat actcaagctt ctatttataa ctcaattata atgggttacc ctgaaacagg
     3721 attaaaaatt gaaggtacta atacatcaca aaatgcgatg aacgatagct tacaatttaa
     3781 aaataatatc ctttcaggaa atgttgacga ttatatttgt acaacatgtg atgttggatt
     3841 taatattgat acttgggcag catcaaatag caaccaaaca tatttaacca acaacttagt
     3901 aggtttagta gatgtattta atttaagtaa tcctgatccg cgtccaactg caggttctgc
     3961 acctacaaca attggaactt atttttcagg tttattaagt gaccctttct ttacaccaac
     4021 ttcttataca ggtgcatttt caacttcaga caattggact gacgaatctt gggtaaattt
     4081 tgacccacaa aatacacctt atactactgc aggtattaat aataaccctg ttgtaactgc
     4141 tactgtaact aacgcaagtt gtgcaggaac aggtgcaatt gatgtatcag taacaggtgg
     4201 tgccggtggt tacacttatt cttggagcga tggtcctaca acacaagacc gtagtttctt
     4261 aaacgcagga acttacactg taactgttac cagcaatggt tgttcagttg taaaatcata
     4321 cacagttaat gatgttacag ttgcaaaacc taccagttta gcaattt
//
LOCUS       DAORKW010000290         4364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_950_length_4364_cov_3.942678, whole genome shotgun sequence.
ACCESSION   DAORKW010000290 DAORKW010000000
VERSION     DAORKW010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_950_length_4364_cov_3.942678"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="PLB46_11035"
     CDS             <1..1208
                     /locus_tag="PLB46_11035"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77098.1"
                     /translation="GGLFPNGANAEDPTTKHYKKGRNIAKNIRALDSLGNITSDSGVV
                     LMAGFGPSIPGHMLDHFVPLVRDTSDEEYQTNICFDAINMGAGGKGLDYAIGADSTKY
                     WNQLLKKIEDKGYTPEQLQISWMYFNDKYDSLNESTFPETPERIADDLTFYIHEMLER
                     FPNVKIVFISGRHYGGFADTTLEQYSAISEPSSYWNNFAVKWLIERQINGSPELKYFG
                     PAMNTPFITWGPYYWSDGNIPRATDGVLYQCSDFSATDGYHCTDSTYERDAHYLMDLI
                     YNSEFSKWYVKDGVSWSDCVPYLDSIHRTQPDIIDINPTGITLFPNPASENINVYRHN
                     ATGKLYAVEIYNQLGQLVYQESGDGVLTNNLAIDIADLNNGMYFLRVAIDDSATGKLQ
                     WLQQKFIKQ"
     gene            1373..2704
                     /locus_tag="PLB46_11040"
     CDS             1373..2704
                     /locus_tag="PLB46_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77099.1"
                     /translation="MLLLLSNANETLRAQGVICSNDSTGLIPLNDLGTGTYEGFQGGL
                     YPFGSNAEDPISTHYKKGKTYAKNLKPLDGAGNINYDNGVVLMAGYGPSLPGQIMNKF
                     VQIVRDTLDSDYHTNPCFDAMNLCIGGKGLQVAIGDGADTYWETIVEKVIEKGYTTEQ
                     VQVGWMYFNDKYDSLAPPQTFPETPEQVTDDLITYLHILMDYFPNMKIMYLSGRHYGG
                     FADTLLEQYPAISEPSSYWNNFSIKWLIERQINADPALKYYGAGVKAPFVTWGPYFWV
                     DGETPRATDGMQYTCSVYNPEDGYHLIDSMYDIEARKLLTHMVNSDFGKNYVKDGVAW
                     ANCTLYADTSLRTIPETLHINDDGIMLYPNPATETINFYRHDAKGNVYTIELYNNIGA
                     LVYSESAAGVAYNNIAIDIATLPTGMYTLRVLVDDALSGNQHWVQEQFIKQ"
     gene            2817..4040
                     /locus_tag="PLB46_11045"
     CDS             2817..4040
                     /locus_tag="PLB46_11045"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HRF77100.1"
                     /translation="MPNPKLRLSFSLLLLTTTIFISGCKNGLITEPKELDFEFRGEII
                     SIELLHSWTIAQTDSALSAYDPDLLTYPNDFPIDIYRIVYKTVDAFNNETQASGAVVL
                     PQGSDVAFPLCNYAHGTVMNRYDVPSYESDELVLGILFGCAGYIMCLPDYIGMGDSPG
                     RHLYCHAKTEATASIDLLRAAKTLADSKTIALNEQLFIFGYSQGGHAAMATTKEIQEN
                     HVGEFTITASAPMSGPYDMDGAQTDFVIADQPYGAPFYLPYLMFAYNEVYQMYDSPSD
                     YLASPYDTLLPPLFDGNHEGWEVDAVMPSIPKNIIKPEVLDDFINNANNPFRVALKDN
                     DLTDWTPTIPMIMYYCGADELVNYQNSLNAQAAFEARGATTTSVYQPSPSASHGDCAE
                     PCFIFANIWFETLKE"
     gene            complement(4091..4309)
                     /locus_tag="PLB46_11050"
     CDS             complement(4091..4309)
                     /locus_tag="PLB46_11050"
                     /inference="COORDINATES: protein motif:HMM:NF015770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HRF77101.1"
                     /translation="MDELVVKDSNGAVLQDGDTVMVVKTLKVKGSSITIKQGTIVKKI
                     RLTDDEELIEGKVEGSVVVLRTEFLKKK"
BASE COUNT         1363 a          795 c          841 g         1365 t
ORIGIN      
        1 agggaggttt attcccaaat ggtgcaaatg ccgaagaccc tactaccaaa cattataaaa
       61 aaggaagaaa cattgccaaa aacatcaggg cactcgacag tttaggtaat attacctctg
      121 atagtggagt agtattaatg gctggttttg gtccctctat tccagggcat atgttggatc
      181 attttgtgcc tttggtgcgc gatacttcag atgaggaata ccaaaccaat atatgttttg
      241 atgccattaa tatgggcgct ggcggcaaag gattagacta tgcgattggt gccgactcga
      301 ccaaatactg gaatcagtta ctaaaaaaga ttgaagataa aggatatact cctgagcaat
      361 tacaaatcag ctggatgtat tttaatgata aatacgacag tttaaatgag agcacttttc
      421 ctgaaacacc ggagcgcatt gctgacgatt taacttttta tattcatgaa atgttagagc
      481 gttttccaaa tgtgaaaata gtatttatca gcggtcgcca ttatggtgga tttgccgata
      541 caacgctcga acaatattcc gctattagtg agccatcatc ttactggaat aattttgcgg
      601 tgaaatggtt aatcgagcga cagataaatg gaagtcccga attaaaatat tttggacctg
      661 ccatgaatac accatttatt acgtggggac catattattg gtccgatggt aatatcccaa
      721 gagcaactga tggtgtgtta tatcaatgca gtgattttag tgcaacggat ggctatcatt
      781 gcacagattc cacatacgaa cgtgatgcac attatctgat ggatttgatt tacaattccg
      841 aattttcaaa atggtatgta aaagatgggg taagttggtc cgattgtgtt ccttacttag
      901 attctattca tcgcacacaa cctgatatca ttgatattaa tccaaccggt attacattat
      961 ttcctaaccc ggcttcagaa aacattaacg tctaccgcca taatgcaaca ggtaaattat
     1021 atgcagttga aatttataat caactcgggc aattggttta tcaggaatca ggcgatggtg
     1081 tattaaccaa taatttggct attgacattg cagacttaaa taacggcatg tattttttac
     1141 gagtagccat tgatgattcg gcaaccggta aattgcagtg gttgcagcag aaatttatca
     1201 aacaataaca tattattacc cccggtgcat atagtgccgg ggttttattt ctctcctcta
     1261 gtttataggt atccgattgt gattttattt ttttagataa aattattatc tttattgtat
     1321 ccacaagacc tatcacatgc acacaaaaca caaactgcca atactattgg ccttgctatt
     1381 gctgcttagc aatgcaaatg aaacactacg agcacaaggt gtaatttgtt caaacgactc
     1441 aaccgggtta attccattaa atgacctggg aaccggcact tacgaaggtt ttcagggcgg
     1501 attgtatcct tttggcagta atgctgaaga ccctatttct acccattaca aaaaagggaa
     1561 aacgtatgca aaaaacctca aaccgttaga cggcgccggg aatattaact acgataacgg
     1621 agtagtccta atggcaggat atggcccatc gttgccgggg caaattatga acaaatttgt
     1681 ccaaattgtg cgtgacacgt tagatagtga ctaccatact aatccttgtt tcgatgctat
     1741 gaatttatgt atcggaggca aaggattgca ggtagctatt ggcgatggcg cagacaccta
     1801 ttgggaaact attgtcgaaa aagttattga aaaaggctat actactgaac aagtgcaggt
     1861 tggttggatg tactttaacg ataaatatga ttctctggca ccacctcaaa cttttccgga
     1921 aacccctgag caggttactg atgatttaat tacctattta catatactga tggattattt
     1981 ccctaacatg aaaattatgt atttgagtgg tcgccactac ggcggatttg cagatacttt
     2041 attggaacaa tatcctgcta tttcagaacc atcttcctat tggaataatt tttccataaa
     2101 atggttaatt gaacgccaga taaatgcaga cccggcttta aaatattatg gcgccggcgt
     2161 taaagcacct ttcgtaacat ggggacctta tttttgggta gatggggaaa cacctcgcgc
     2221 aacagatggc atgcaatata cctgcagtgt atacaaccct gaagatggtt atcatttaat
     2281 tgattcgatg tatgatatcg aagcgagaaa attattaacg catatggtaa attccgactt
     2341 cggaaaaaat tatgtgaaag atggtgtagc ctgggcaaat tgtacattat atgctgatac
     2401 cagtttaaga acaattcctg aaacattaca tattaatgat gacggtatca tgttgtatcc
     2461 aaacccagca actgaaacaa ttaattttta ccgacatgat gcgaaaggta atgtgtatac
     2521 aattgaattg tataataata tcggcgcttt agtttacagc gaaagtgctg ctggtgttgc
     2581 ttacaacaat attgctatag atattgctac gttgccgaca ggtatgtaca cattacgtgt
     2641 attagttgac gatgctttat cgggtaatca acattgggtg caagagcaat ttatcaaaca
     2701 ataacctatt taaaggcatt caaattttga atgccttttt ttttatacaa gcacaaaata
     2761 tgttctattg ctgagcatat tgttacaatt gagtatcttt atctaaacat taccacatgc
     2821 ctaatcctaa attacgcctt agtttttcac tattattgtt aactacaacc atttttattt
     2881 ccggttgtaa aaacggttta attacggaac cgaaagaact ggattttgaa tttcggggtg
     2941 aaataatcag tattgaatta ttacacagct ggacgattgc acaaacagat tctgcgcttt
     3001 cagcttacga tcctgattta cttacttatc cgaatgattt tccgatagat atttatcgta
     3061 ttgtttataa aacggtagat gcttttaata atgaaacaca agcttcaggt gcagttgttt
     3121 taccacaggg tagtgatgta gcatttccat tgtgtaatta cgcacacggc actgtaatga
     3181 accgatatga tgttcccagt tacgaaagtg atgagttggt attaggcata ttattcggct
     3241 gtgcaggata tataatgtgt ttacccgatt atattggtat gggagattcg cctggcagac
     3301 atttatattg tcacgcaaaa accgaagcca ctgcaagtat cgatttatta cgtgctgcaa
     3361 aaacgctggc cgattctaaa acaattgcac taaatgaaca attattcata tttggttatt
     3421 ctcagggcgg acatgctgct atggctacca caaaagaaat tcaagaaaat catgttggtg
     3481 aatttacgat tactgcaagt gcaccaatga gtggtcctta tgatatggat ggcgcacaaa
     3541 ctgattttgt aattgccgac cagccgtatg gtgcgccttt ttatttgcca tatttaatgt
     3601 ttgcgtataa tgaagtttac caaatgtatg atagcccttc cgattattta gcttcaccat
     3661 atgatacttt attgccacca ttatttgatg gtaaccacga aggatgggaa gtggatgcag
     3721 taatgccgtc cattcctaaa aatattatca aacctgaagt gttggacgat tttattaata
     3781 atgcgaataa ccctttcaga gttgcattaa aggataacga tttaactgac tggacaccaa
     3841 caattcccat gattatgtat tactgtggag ccgatgaatt ggtgaattat caaaattcgt
     3901 taaatgctca ggcggcattt gaagcaagag gtgcaactac tacttctgta tatcagcctt
     3961 caccaagtgc atcacatggc gattgcgccg aaccatgttt tatatttgcc aatatctggt
     4021 tcgaaacatt aaaagaataa ccgataaaaa ttcccgaaat aaataataaa ccgattgcca
     4081 aacagtcggt ttatttcttt ttaaggaatt cggtacgcaa cacaactacc gaaccttcta
     4141 cctttccttc aattaattct tcatcatcgg tcaatcgtat ttttttgaca attgtacctt
     4201 gtttaatggt gatggaagag cccttaactt ttaacgtttt tactaccata acagtatcgc
     4261 catcttgtaa tactgcacca ttgctgtctt taactactaa ttcgtccata tctgtaaatt
     4321 aagtcgcaaa attaaaatta tttattatat gcacgaaatt gaaa
//
LOCUS       DAORKW010000291         4363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_951_length_4363_cov_4.181058, whole genome shotgun sequence.
ACCESSION   DAORKW010000291 DAORKW010000000
VERSION     DAORKW010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_951_length_4363_cov_4.181058"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..3256
                     /locus_tag="PLB46_11055"
     CDS             128..3256
                     /locus_tag="PLB46_11055"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77102.1"
                     /translation="MKKLLFAVAVCLPFFSQAQKVAKPPVAALPFDTTITAELKFRNI
                     GPWRGGRSTAVVGDLQNDQLFYFGATGGGVWKTNDGGSNWKNISDGFFGGSVGSIAVS
                     KSDPSIIYVGMGENTMRGNVSEGFGMWKSENGGRSWKNVGLNDTRHIMRIVIHPKDEN
                     VIYAAALGHLFGPNDERGVFRSKNGGQSWEKILYVNDQVGACDLVIDPSDPNILLATF
                     WNVKRTPFSLESGGPGSGIYKTIDGGNNWTNITKNKGLPQDTLGIIGITISASNPEIY
                     YAIIESKTGGIFKSIDAGKTWTKTSDNSNQRQRAWYFSKIFCDPKNENVVYVLNVEFW
                     KSTDGAKTFKSISTPHGDHHDLWIDPNNPQRMIIGDDGGAQTSFDGGNNWSTYHNQPT
                     AQIYRVSTDNAVPYRIYGAQQDNTSLRIRHRSRGGQIDDNDWEPTAGFESGFIVANPL
                     NSDIVYGGNYSGFIGCLNHKTGDARNITVWPNDPIGQGAGTQQYRFQWNFPLFFSPHN
                     PRRLYAAGNVLFYTEDEGSTWVAMSPDLTTNDKSKQQPSGGIITKDNTGVEVYCTIFA
                     AAESDLEKGLIYTGSDDGLLYVTKDDGKNWEQITPTNMPKDMMINCIEIDPFETGKMY
                     FAGTRYKSDDFAPYVYMTSDYGKTWSKITNGIDNLHFTRAIRADKKRKGLLFCGTEYG
                     LYVSYNYGANWSPFQLNLPIVPITDMTFKNNDLIIATQGRGFWIFDDVAIFQQMEPNW
                     SAKGFQVLPMRDVPRIDGYQIENPVNAGKNPMPGAVVNYYIPYLKNKAGNDSIPDVEI
                     NIYDNDEKKVNTYSTKATEAAYKINNVKHGLNAFSWNFQYSGGVNIDGMFLWNGTPGG
                     PLAAPGNYAFVVKIGNDSIRQDCFILPDQNSQAAMEDYRLQHKFLIEVRDKFNETQNA
                     IKDIWALQAQMDAYVNKLGESAPQQLKDSVTAIKDDLSAIVDVLYERRNKSVQDMLNY
                     GIKLNDKLAGVYNAASYGNFKPSANQYLVYEDLKEQINRQLELYQDITVNRINALNKM
                     IIGMSLPVIISE"
     gene            3452..4201
                     /locus_tag="PLB46_11060"
     CDS             3452..4201
                     /locus_tag="PLB46_11060"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HRF77103.1"
                     /translation="MKNLALITLLLLSCTSFAQVINIEDKRIKTDTIGWAGSAEAAVY
                     LNKNNDFVLSFNTDIHIQYKNKKSLFLLLTDIASVQVNETQSFVNSGFQHFRYNYKIT
                     DRFVWEAFVQGQYNEPLAIDYRILVGTGPRYKIIGADKFRLYAAALYMNENEQNTGVE
                     MVQNNHRLSSYVSFTLSPNDKFSVVSTTYFQPLFNDFSDQRISTSFDLKSYLTKKLYL
                     DLNYNFLDDSKPAEGVVNTIYEMTAGIGLEF"
BASE COUNT         1426 a          698 c          906 g         1333 t
ORIGIN      
        1 caatactgct ttttccgtcc caccaaccac gctttcaatt aaaatccgtt aaatccacaa
       61 tatccgcatc ccaaattttt gtaatccgat tgcctttcgg ttacttagtg ccaccaatct
      121 atttactatg aagaaattat tatttgcagt tgcggtctgc ttaccctttt tttcccaggc
      181 tcaaaaggtt gcaaaaccac cagtagctgc gttgccattt gataccacca taacggcgga
      241 actcaaattc agaaacatag gaccatggcg aggaggccga agcaccgcgg ttgtcggcga
      301 tttgcaaaat gaccaattgt tttattttgg tgctaccggc ggtggggttt ggaaaacaaa
      361 tgatggcggc tccaactgga aaaatatttc tgatgggttt tttggcggtt cagttgggag
      421 tatagcagta agtaaatcgg atccatcaat aatttatgta gggatgggtg aaaacaccat
      481 gcgtgggaat gtgagtgaag gatttggtat gtggaaaagc gaaaatgggg gtcgttcatg
      541 gaaaaatgtt gggcttaatg acactcgaca tatcatgcgg attgttattc accccaaaga
      601 tgaaaatgtt atttatgcgg cagcacttgg tcatttattc ggacccaatg atgagcgagg
      661 ggtgtttcgt tcgaaaaacg ggggacaatc ttgggagaaa attttatatg taaacgatca
      721 ggtgggtgct tgcgatttag tgattgaccc aagtgaccct aatattttat tggcgacttt
      781 ttggaatgta aaacgcacac ctttttcgct ggaaagtggt ggtccgggtt cgggcattta
      841 taaaactatt gatggtggaa ataattggac taatatcaca aaaaataaag ggctgcctca
      901 agatacttta ggtataattg gcattacaat ttcggcaagt aatccggaaa tttattatgc
      961 tataattgaa tctaaaacgg gtggtatatt taaaagtatt gatgccggaa aaacttggac
     1021 aaaaacatct gataacagta atcagcgtca acgtgcctgg tatttctcca aaatattttg
     1081 cgacccgaaa aatgaaaatg ttgtttatgt gctgaatgtt gaattttgga aaagcaccga
     1141 tggggctaaa acatttaaat cgattagcac accgcatggt gatcatcacg atttatggat
     1201 tgatccaaat aatccacaac gtatgattat tggtgatgat ggcggcgcac aaacatcctt
     1261 cgacggtgga aataattgga gtacttatca caatcaaccc acggctcaaa tttatcgcgt
     1321 gagtaccgac aatgcagtgc catatcgcat ttatggtgca cagcaggata atacttcatt
     1381 acgtattcgt caccgaagca gaggcggaca aattgatgat aacgattggg aaccaactgc
     1441 aggttttgaa agtggattta tagttgccaa tcccttaaat agtgatattg tttatggcgg
     1501 aaattattcc ggatttattg gttgtttaaa tcataaaacc ggtgatgcac gaaatattac
     1561 cgtttggcca aacgacccga taggacaggg ggcaggaact cagcaatatc gttttcagtg
     1621 gaattttccc ttgttttttt caccgcataa ccctaggcga ttatatgctg ccggtaatgt
     1681 attgttttat acagaagatg aaggttcaac ttgggtagcc atgtcgccgg atttaaccac
     1741 gaatgataaa tcaaaacaac aaccaagtgg tgggattata acaaaagata ataccggagt
     1801 tgaagtgtat tgcaccattt tcgcagcagc agaatcggat ttagaaaaag gattaattta
     1861 taccggaagt gatgatggat tattatatgt aactaaagac gacggtaaaa attgggagca
     1921 aattacacct accaatatgc caaaagatat gatgataaat tgtatcgaaa ttgacccatt
     1981 tgaaaccggt aaaatgtatt ttgcaggaac cagatataag tcagatgatt ttgctccata
     2041 tgtttatatg acaagcgatt atggtaaaac ttggtcaaaa attactaatg ggatagataa
     2101 tttacatttt acacgtgcta ttcgcgctga taaaaaacgc aaaggattat tattttgcgg
     2161 aacagaatac ggtttgtatg tatcctataa ttatggtgct aattggtcgc cgtttcaatt
     2221 gaatttacca attgtcccaa taacggatat gacatttaag aacaatgatt taattatcgc
     2281 cactcaagga cgtggttttt ggatttttga tgatgtagca atatttcaac aaatggaacc
     2341 caattggagt gcgaaaggat ttcaggtgct accgatgcgt gatgtacctc gcattgatgg
     2401 ttatcaaata gaaaatccag ttaatgccgg taaaaatccg atgccgggtg ctgtagttaa
     2461 ttattacatt ccttatttaa aaaacaaggc aggaaatgat tctattcctg atgtggaaat
     2521 taatatttat gataacgatg aaaaaaaagt aaatacctat tccaccaagg caacagaagc
     2581 tgcctataaa ataaataacg taaaacatgg cctaaatgca ttttcatgga atttccaata
     2641 tagtggaggt gtaaatatag atggtatgtt tctatggaat ggaactccgg gaggaccgct
     2701 tgctgcaccc ggtaattatg catttgttgt aaaaatagga aatgacagta tccgtcagga
     2761 ttgttttatt ttacctgatc agaattctca ggcggcaatg gaggattatc gtttacaaca
     2821 caaattttta attgaagtaa gagataaatt taatgagacg caaaatgcaa ttaaagatat
     2881 ttgggcattg caagctcaaa tggatgctta tgtaaataaa ttaggagaat cagcgccaca
     2941 gcaactgaag gattctgtca cagcaattaa agatgatttg tcggcaattg tggatgtatt
     3001 atatgaaagg cgtaataaaa gtgttcaaga tatgctgaat tatggtatta aattaaacga
     3061 taaattagca ggtgtttaca atgcagcctc ttacggaaat tttaaaccat cagctaatca
     3121 gtatttggtg tatgaggatt taaaagaaca aattaatagg cagttggagt tatatcagga
     3181 tattactgtt aacagaataa atgccttaaa taaaatgatt attggaatga gtttgccggt
     3241 gataatttcg gaatgatggt caattttatg acggttattt aattttagga agggcaaaaa
     3301 tgattgagta atagagtttt ggtgagacaa gctaacacgt gtatttaagt tttatccgat
     3361 tacatgaaac gctagtgtgg tatagttcgc aattggtatg ctgcatgcaa attttcattt
     3421 tttatcctta attttgcaaa aaatgataat catgaaaaac ctagccctca ttactttact
     3481 gttgctcagt tgcacctctt ttgcgcaagt tattaatatt gaagataaaa ggataaaaac
     3541 ggatacaata ggttgggcag gtagtgctga ggctgccgtt tatctcaata aaaataacga
     3601 ctttgtgttg tcgtttaata cggatattca tattcaatat aaaaataaaa aaagcctgtt
     3661 tttattgtta accgatattg ccagtgtgca ggtaaatgaa acacaaagtt ttgtgaattc
     3721 cggatttcaa catttcaggt ataattataa aatcaccgat cgttttgttt gggaggcttt
     3781 tgtacaggga cagtataatg aaccattggc aatcgattac cgaattttgg tgggaaccgg
     3841 tccgcgttat aaaattatag gtgcagataa attcagattg tatgccgcag cattatatat
     3901 gaatgaaaat gaacaaaaca ccggtgttga aatggtgcaa aataatcatc gcttaagcag
     3961 ttatgtttca ttcacattat caccaaatga caaattttct gtagtgagta ctacatactt
     4021 tcaacctttg ttcaacgatt ttagtgacca aagaatttcg acaagttttg atctaaaaag
     4081 ttatctcacc aaaaaattat atcttgacct caactacaat ttcctagacg actcaaaacc
     4141 ggccgaaggt gttgtaaata ctatttatga aatgacggca ggaattgggc tggagtttta
     4201 attagttaat tagctgatta gcaaattagc taatggttac tttttcgaaa cctttacaat
     4261 ttggattccc cttatcattt tgcaatattc cgccatggcg gaatattatt taaaattcga
     4321 agctgtactg taaaattatt tactaaaccc catttgctaa tta
//
LOCUS       DAORKW010000292         4354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_959_length_4354_cov_4.180274, whole genome shotgun sequence.
ACCESSION   DAORKW010000292 DAORKW010000000
VERSION     DAORKW010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_959_length_4354_cov_4.180274"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /gene="secF"
                     /locus_tag="PLB46_11065"
     CDS             <1..722
                     /gene="secF"
                     /locus_tag="PLB46_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRF77104.1"
                     /translation="KLKITTGYMIDNPDPGADSLVAQALYKELSGLYTGVDFKTWDST
                     FKESQMKVEPTIADDIQASALKAIGLALIAIFIYIVIRFRKWQFGAGALASLIHDAFM
                     VLAMFALLKNVMPFSLEINETFIAAILTVIGYSINDTVVVFDRIREYLRESKAGTVPE
                     IFNKAINDTLSRTMITSGTTLLSIIVLLIFGNENIQGFAFALFVGIGFGTYSSVFVAS
                     AIAVDMYKKEDHNKSAEDVMK"
     gene            1010..2827
                     /locus_tag="PLB46_11070"
     CDS             1010..2827
                     /locus_tag="PLB46_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF77105.1"
                     /translation="MARYYDKALIRLQNAINEEREFVTAWQYLGDTYRNMKNDSMAVE
                     AYLKTLELDPDRDVILYSKLAESEGNIGMYEEAYAHMVIFLQNPDVKGDLKTKAIKLR
                     KNYEFAKEAVKNPVNFNPYNMGNEINSDFPEYFPSLSVDGKTMVMTRRIDDVISLGPG
                     DNRKIEQEDFYISFLENGAWTPAQNMGAPINTKMNQGAQSISADGRFLFYTSCEDLEN
                     GYGSCDLYYSLKIGNIWSNPENCGTIINTADWESQPSVTADGRELFFSSGRPGGKGSY
                     DLWHAKLGEDGYWMEPENLGDVINTPYLEQCPFIHPDGKTLYFASEGHPGMGSADLFY
                     STRDENGIWSKPINLGYPINTQNREISLSVSSNGTTAYFSSDRDKTLGDLDIYSFELP
                     PAVQAEQVTWVNAIVTDAKTKQPLKASVQLMNIATGEIVASSNSDPKTGEFLIVLPSG
                     NDYGLFVQREGYLFHSENFSLKNVMPDAPYLIKVGLQPINAGEILTLKNIFFETASAE
                     ILDVSEPELNKVVELMQKNPNMKIKVNGHTDNVGADADNLKLSLNRSLSVKNYLIGKG
                     IDASRISYQGFGESKPIDVNTTETGRANNRRTEIEIISI"
     gene            2932..3840
                     /locus_tag="PLB46_11075"
     CDS             2932..3840
                     /locus_tag="PLB46_11075"
                     /inference="COORDINATES: protein motif:HMM:NF025007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HRF77106.1"
                     /translation="MSIDVSKPIPIVDGQKISREEFEKNYVKPQKPVILRGLWKQYPA
                     YAKWNYAFFKTTMGDVEVGLYSSTKADPSKTMSEPTVKMKFSEYLQLIEKEPTDLRLF
                     LFPIFKYKPELLKDFDYPAITKNYVKLPFMFFGPKNAITRMHQDIDMSNVFLTQFEGK
                     KRVVLFPPDQSDLLYKLPFNVHSTVDIDKPDYKEYPALKYAKGLTGILEYGDTLFMPS
                     GYWHHIEYLEGGYGLSVRSLPPQFSIKLKGFYNLTIQRKLDDILRFTFGNKWFELKKS
                     IAKKRAERAIKRIHKHNEHSGDLAFQ"
     gene            3929..>4354
                     /locus_tag="PLB46_11080"
     CDS             3929..>4354
                     /locus_tag="PLB46_11080"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HRF77107.1"
                     /translation="MCLINLAFRQHPSYPLILVANRDEFYARPTRILQWWEDEHPDLL
                     AGKDLQEGGTWMGINKQGQFAALTNYRDLSNINNNAPSRGHIVKRYLNGELPDEEMFR
                     FLKTQGKLYNGFNIIYGNVDMLYYYSNVTDQVRNLYPGIY"
BASE COUNT         1416 a          840 c          850 g         1248 t
ORIGIN      
        1 acaaattaaa aattactaca ggctatatga tcgacaatcc ggatccgggt gctgattcat
       61 tagttgcaca ggcgttatac aaagaattat caggtttata taccggcgtt gatttcaaaa
      121 cttgggattc aacatttaaa gaaagccaaa tgaaggtgga acctaccatc gctgacgata
      181 ttcaggcttc agcattaaaa gcgattggtt tggctttaat tgccatcttt atttatatcg
      241 ttatacgttt ccgtaaatgg cagtttggtg ccggtgccct tgcatcatta attcacgatg
      301 cattcatggt gctcgctatg tttgccttgc tgaaaaatgt gatgcctttc tccctagaaa
      361 taaacgaaac atttatcgct gccatattaa cggtgattgg atattccatc aacgataccg
      421 tggtggtatt cgaccgtatt cgtgaatact tgcgggaaag caaagcaggc acagtgcctg
      481 aaatctttaa caaagcaatt aacgatacct taagtcgtac catgattact tcaggtacta
      541 ccctcctttc cattatcgta ttattgattt ttggtaatga aaacattcaa ggcttcgcat
      601 ttgcattgtt cgttggtatt ggatttggta cttattcatc agtatttgtt gcatctgcaa
      661 ttgcggttga tatgtataaa aaagaagatc ataacaaatc agcagaagac gtaatgaaat
      721 aattacaatt ccgcttaact tatacatagg ttgtctcaaa aggacaacct attttttttg
      781 tgtgcagatg aacagtatac acacctgttc atctgcacaa cccaaaaaat agaacgattt
      841 ttgcagatat acatttaacc ttcatcttta cgttattatg caaatgatta aaccactgct
      901 gattgccctt ttattgtgct gcacaacagc tgcaattgcg caacccgtta cagaagccaa
      961 cgcttccaat aaggcaatga aatgccttga gtaaggtggt caacgcgcta tggcgagata
     1021 ttacgacaaa gcgctaatta ggttgcaaaa tgccattaat gaagagcgcg aatttgttac
     1081 agcatggcaa tatttaggcg atacctaccg caacatgaaa aatgactcca tggcagtgga
     1141 agcgtatcta aaaacactgg aactggaccc tgaccgagat gtaattttat attccaaatt
     1201 ggcagaatcg gaaggaaata ttggcatgta cgaggaagct tatgcacata tggtcatttt
     1261 tctgcaaaac cctgatgtta agggagattt aaagacaaaa gccatcaaac tgcgcaaaaa
     1321 ctatgaattt gctaaggaag ctgtcaaaaa tcctgtaaat tttaatccct acaacatggg
     1381 aaacgaaata aactctgatt tccctgaata tttccctagt ttgagcgtag acggcaaaac
     1441 catggttatg acacgcagaa ttgacgatgt gatatcatta gggcccggag ataaccgtaa
     1501 aattgaacag gaggacttct acatctcgtt tttggaaaat ggggcttgga ccccggcaca
     1561 gaatatgggt gcgcctatca acactaaaat gaaccaggga gcccaaagca tttcagctga
     1621 cggtcgattt ttgttttata cctcctgcga agaccttgaa aatggctatg gcagctgcga
     1681 tttatactat tccttaaaaa tcggcaatat ctggtctaac ccggaaaact gcggaacaat
     1741 tatcaataca gcagactggg aatcacaacc ttcagtaaca gccgatggtc gagagttgtt
     1801 tttcagctca ggacgccccg gaggcaaagg cagctacgat ttatggcatg ctaaattggg
     1861 agaagatggt tattggatgg agcctgaaaa tttgggggat gttatcaata caccttacct
     1921 ggagcaatgt ccgtttattc accctgacgg taaaaccttg tattttgcca gtgaaggaca
     1981 tcccggaatg ggctccgccg atttatttta ttcaactaga gatgaaaatg gcatttggtc
     2041 aaaacctatt aacctcggct acccaatcaa tacccaaaac agggaaatta gtttgtcggt
     2101 gtcctcaaat ggcacaacag cctatttttc tagcgacaga gataaaacac tcggcgacct
     2161 cgatatttat tcgtttgaat tacctcctgc tgtgcaagcg gaacaagtaa cttgggtaaa
     2221 tgccatcgtt accgatgcca aaactaaaca accactcaag gcatctgtgc aattgatgaa
     2281 tattgccaca ggtgaaatag ttgcctcatc taattctgac ccaaaaacgg gcgaattttt
     2341 gattgtatta cccagcggaa acgactatgg cttatttgtt caacgcgaag ggtatttatt
     2401 tcactccgaa aatttttcac taaaaaatgt aatgcccgat gcaccttatt tgataaaagt
     2461 cggtttacaa cctatcaacg ccggtgaaat cttaacatta aaaaatattt tctttgaaac
     2521 ggcgtctgct gaaatattag acgtttcaga acctgagctg aataaagtgg tggaattaat
     2581 gcaaaaaaat cccaacatga aaataaaggt gaacggacat accgataacg ttggtgcaga
     2641 tgccgacaat ttaaaattat cattaaatcg ttccctgagt gtgaaaaatt acttaattgg
     2701 caagggaatt gatgcatcta gaataagcta ccagggattt ggtgaaagca aacccataga
     2761 cgtaaatact accgaaaccg gcagagcaaa taaccgcaga actgaaattg aaattattag
     2821 tatttaatac tgaataattg tagtagttaa aaataacata attgaataac cacattaaca
     2881 aaacctaatt acccgataca ccaattaatt ttaaaaccaa aatccgatgc tatgtcaatc
     2941 gatgtttcga aacctattcc gatagtggac ggacaaaaga tttcccgcga ggaatttgaa
     3001 aaaaattatg taaaaccgca aaaaccggtc atacttcgtg gcttgtggaa gcagtatccg
     3061 gcctacgcca aatggaatta cgcctttttt aaaactacaa tgggcgatgt ggaagtgggc
     3121 ctttacagca gcacaaaagc cgacccgagt aaaactatgt ccgaacctac cgttaagatg
     3181 aagtttagtg aatatcttca gcttatcgaa aaagaaccaa ccgacctgcg cctgttttta
     3241 tttcctattt ttaaatacaa accggaatta ttaaaagatt tcgattatcc tgccatcaca
     3301 aaaaattatg tgaaattacc gttcatgttt tttggcccta aaaatgccat tacccgtatg
     3361 catcaggata tcgatatgag taatgttttt ttaacgcagt ttgaagggaa aaaacgtgtg
     3421 gtattatttc ctccggacca aagtgattta ttatataaac tgccttttaa tgtacattca
     3481 actgtcgata ttgacaaacc tgattacaag gaatatccgg cattaaaata tgcgaaagga
     3541 ttaacaggta ttttggaata tggcgacacc ttatttatgc cttcaggata ctggcatcat
     3601 attgaatatt tggaaggcgg atatggttta agtgtgcgta gtttgcctcc acagttttcg
     3661 attaagttaa aagggtttta taatttaacc attcagcgca aacttgatga tattttgcgt
     3721 tttacattcg gtaataaatg gtttgaactc aaaaaatcta tcgctaaaaa aagagcggaa
     3781 cgcgctatca aacgtatcca caaacataat gaacacagtg gggatttagc ttttcaataa
     3841 attatagcaa caccttagtt aatccatctg ccctggcgga tggatttttt attgctgtta
     3901 atcctaaaaa gtaattgtaa ttttaaacat gtgtttaatt aaccttgctt tcagacaaca
     3961 tccctcctat ccattaattt tggttgcgaa ccgtgatgaa ttttatgcac ggccaacacg
     4021 aattttacaa tggtgggaag atgaacatcc cgacctgttg gcaggcaaag atttacagga
     4081 aggtggcacc tggatgggca tcaacaaaca aggacaattt gcagcactaa ctaattaccg
     4141 cgatttatct aatataaaca ataatgctcc ctctcgcgga catattgtga aacggtattt
     4201 aaacggtgaa ttaccggacg aagaaatgtt tcggttttta aaaacgcagg ggaaattata
     4261 caacggtttt aatatcattt acggcaacgt tgacatgtta tattattatt cgaatgtaac
     4321 cgaccaggtg cgaaatcttt atccgggcat ttac
//
LOCUS       DAORKW010000293         4351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_960_length_4351_cov_5.430866, whole genome shotgun sequence.
ACCESSION   DAORKW010000293 DAORKW010000000
VERSION     DAORKW010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_960_length_4351_cov_5.430866"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1019)
                     /locus_tag="PLB46_11085"
     CDS             complement(180..1019)
                     /locus_tag="PLB46_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF77108.1"
                     /translation="MKSSTTGLNLMLNINNVNLYYDDAGEGNVPIIFLHGFPFDKSMW
                     AQQIAFFATTNRVIAIDIRGFGKSSAGTAPLSIDLFSEDLMLFMNQLNISKAIICGLS
                     MGGFIALNAQSRFPDRFEAIILCDTQCIADTAEVKSNRYKAIDEISLNGTLNFNEAFI
                     KKVFCENSLTNKTEVVKKLQNVVFANNEESIINGLKALAERAETCSTLAQINIPTLII
                     CGREDEVTPLEQSEFLHASIKESTFHIIDDAGHVSNIEQPEEFNNELSKFLRAVDVFR
                     KRM"
     gene            complement(1704..2585)
                     /locus_tag="PLB46_11090"
     CDS             complement(1704..2585)
                     /locus_tag="PLB46_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRF77109.1"
                     /translation="MLKASRFKFVYLIFCLLTSIVTLVAQTNDSDKHTLNAMRIIGHE
                     LLLDASDSTSRVLPVIKQSNTYTIQFATEFQFLPKQLFETIDSVIKATNIASRYIVEV
                     EVCTTKEIIYSYEAGRSTLAEMIPCAFREPEKACYSVAITILETNVATTSDIKKPYLS
                     IQFWIPGIAVVIIAAVLVIALFRRRKQEEIIKTNGHAILIGAYKFDPVNSVLLTQQGK
                     VSFSAKESELLQLLNKNANTTIEREVILKTVWGDDGDYIGRTLDVFISKLRKKLEANT
                     NLKIVNIRGVGYKLIIA"
     gene            2716..>4351
                     /locus_tag="PLB46_11095"
     CDS             2716..>4351
                     /locus_tag="PLB46_11095"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF77110.1"
                     /translation="MEKWIIQMFLLVVINFITSCGINAATGEPEGAINSKMSSDEKLS
                     VVQRLKESAQLSVEEKVKLYYTLKKDSAQYYNFGNEDELNLFGYAYLWDNKIEEAIII
                     FKLVVAEFPASSNAYDSLGEAYLKNGNLKQAKKNYEKSLALNPDNFNAEDQIELILFP
                     DKVKLSPTEKFKQVFLAEAYKQDLEQLGNTLLKIHPNALKFISKEKFWQAIESKKELI
                     TDSTTFGEFAWMCSEIIANVHCSHTNMGSFNFETSLLMPVQKFPLQTYWVNDKLYVVD
                     AMNNGKSIKVRDEIISINGVNTSTFMSDIYKHIASQGYIKTTKNRMFNMWSSTLMAYA
                     FNFPETYSVKVASSNKEIYLQPATAVEEPYFDESLTYANSVLDLELMPDNSAAYLTIA
                     SFNYYRWNDFDVFKNFIDSSFQVINKNKIDNLIIDLRFNGGGSQSASIHLLRYLLDEP
                     FTYYSNAQFEGKQGKIEGEELIYPFENRFKGKSYFIIDGHGNSTTGHFMSIVKHFDVG
                     TIVGEELGSNQFCSAGMTTCRLSNTKLVYYVANNTHE"
BASE COUNT         1483 a          723 c          704 g         1441 t
ORIGIN      
        1 caatgcagcc ttgatctttt ggttcttttg tatcaagaca aaagaacact aaatcccatt
       61 cgaagctaat aacgccaatt aaagtcaaac taacttgggt ctcttaacaa aaaatcaaca
      121 aatcacttac aagtttaaaa ttcaatcaag aattaaaacc cctccaaagc caaaattaat
      181 cacattcgtt ttctaaatac atcaactgcc cgcaaaaatt tagacaactc attattaaat
      241 tcttccggtt gttcaatatt ggaaacatga ccggcatcat caataatatg aaacgtagat
      301 tccttgatag atgcatgcaa aaactcagat tgttccaaag gtgtcacttc atcttctcta
      361 ccgcaaataa ttaatgtcgg aatgtttatt tgagcaagcg ttgagcaagt ttcagcacgc
      421 tcagcaagag cttttaagcc attaataatt gactcttcat tgtttgcaaa aacaacattt
      481 tgcaattttt ttacaacttc cgttttgtta gtaagcgaat tttcacagaa caccttttta
      541 ataaacgctt cattaaaatt gagtgttccg ttcaaagata tttcgtcaat agctttatat
      601 cgattcgatt ttacttcagc agtgtcagca atacattgcg tgtcgcatag aataattgcc
      661 tcaaacctat ctggaaatct ggactgggca tttaatgcaa taaaaccacc catagataat
      721 ccacaaatta ttgctttgga gatgttcaac tgattcataa acaacattaa atcctcacta
      781 aataaatcta ttgacaaagg cgctgttccg gcgcttgatt tgccaaatcc acgaatgtca
      841 atggcaataa ctcgattagt tgttgcaaaa aaagcaatct gttgagccca catcgatttg
      901 tcgaaaggaa atccatgtaa aaatataatc ggaacattgc cttctcctgc atcatcgtaa
      961 taaagattta cattgttaat attgagcatt aaatttaagc ctgtagtaga acttttcata
     1021 ccttgataat tttagataaa tattgttatt ctattggcaa ttaaaatatt ggtaatttat
     1081 ttagctattc gtaaatatga ggacaattaa tcgtataaca gcaaacttac ttacaacttg
     1141 ttgaccctca cttatataat tttatataaa aatcacataa ttcccacttt taaagtataa
     1201 aaaagggagt tattaatttg gctttaattg gttatgaccg gatgaaatac cgaaatttta
     1261 ttccaggtaa cttgtagtga ctaaagtgct tgtttgtgct ttaatatagg ccttaattga
     1321 gttgatctaa tttgcaggca gccctaaata gtgcttaaga gaatggtaag aacccagtct
     1381 aaactaaatt atatttcaat agcaatttgt tcattatgtt attatgttac ttattttcca
     1441 ttcaatccta atattaaaca atgatttcta gtatttttta attccaaggt agaagaattt
     1501 aaaatacagt tgaaatatac gaaatgccta gtagcctgtc tttatcccaa attagattaa
     1561 aactttattt aaagacaaat tgtgaaataa cagatagtca tagtgtaact ggatgttata
     1621 gttctctgga gtattgatat aacactaagc tactttaatt ttaactttaa attgaataag
     1681 ctttttaaaa ccatttttat tatttacgct attattaatt tgtagccaac tcctctgata
     1741 tttacaattt ttaaatttgt attcgcttct aatttttttc gaagtttaga aataaataca
     1801 tctaaagttc tgccaatata gtcgccatca tcaccccaaa ctgtctttag aattacctcg
     1861 cgctcaattg ttgtatttgc atttttattt aataattgta atagttcaga ctcttttgcg
     1921 ctgaacgata ctttaccttg ctgtgtcaat aaaacggaat tgacagggtc aaatttatat
     1981 gctcctatca aaattgcatg tccattggtt tttattatct cttcctgttt cctacgccga
     2041 aaaagtgcta tcactaaaac agcggcaatt ataactactg caatacccgg aatccaaaat
     2101 tgaattgata aatatggctt ctttatgtcg ctggttgttg caacattggt ttctaaaatt
     2161 gttatggcaa ccgaataaca agccttttcc ggttctctaa aggcgcatgg tatcatttca
     2221 gcaagggttg aacgcccggc ttcatagctg taaattattt cttttgtagt acatacttca
     2281 acttcaacaa tatacctgga ggcaatatta gttgctttaa taacgctatc aatggtttca
     2341 aatagttgtt ttggcagaaa ttgaaattct gtggcaaatt gaatagtgta agtattggat
     2401 tgttttataa cgggtaaaac gcgtgaagta gagtctgacg catctagcag aagttcatga
     2461 cctataatgc gcatggcatt caaagtatgt ttatcactat catttgtttg agcaactaat
     2521 gttacaattg aagtaaggag gcaaaaaata aggtaaacaa atttaaacct agacgctttt
     2581 aacatattgc aaatatagca gcagcaccct aatacagtgg gctttcactg catttttaca
     2641 ctaatttaca ttaagttacc ttgaaaaata ttgtatttta aggaagggtc ttactttcgt
     2701 tctaaatata gcagtatgga aaaatggata attcagatgt ttttgcttgt tgtgataaat
     2761 ttcatcacct catgtggtat aaacgctgct accggtgaac cagagggggc aattaactca
     2821 aaaatgagca gtgatgaaaa actttctgtt gtacaaagac taaaagaaag tgctcaatta
     2881 tctgttgaag aaaaggttaa actttactat acactaaaga aagatagtgc acaatactac
     2941 aacttcggca atgaagatga gctcaaccta tttggttatg cttatttatg ggacaataaa
     3001 attgaagagg caattataat tttcaaattg gtggtggctg aatttcctgc ttcctcgaac
     3061 gcatatgata gtttaggtga ggcctactta aaaaacggca acctgaaaca agcaaaaaag
     3121 aattatgaaa aatcgctagc cttgaatcct gataatttta atgctgaaga tcaaattgag
     3181 cttatcttgt ttcccgacaa agtcaaacta tcacctactg aaaaatttaa acaagtattt
     3241 ttggctgaag cgtataaaca agacctcgag caacttggta atacattgct caaaattcat
     3301 ccaaatgcat taaagtttat ctcaaaagaa aaattttggc aagctattga atccaaaaaa
     3361 gaactcatta ccgatagcac tacttttggt gaatttgctt ggatgtgcag tgaaattatt
     3421 gccaatgtgc attgttcgca taccaatatg ggtagtttta atttcgaaac atcgttacta
     3481 atgcccgttc aaaaatttcc attacaaaca tattgggtaa atgacaaact atacgtggtt
     3541 gatgctatga ataacggcaa atctataaag gttagagatg aaataattag cattaatgga
     3601 gttaatacat ccacttttat gtctgatatt tataagcata ttgcctcaca aggatacatc
     3661 aaaacaacta aaaaccgtat gtttaatatg tggagctcaa cgttaatggc ctacgccttt
     3721 aattttcctg aaacatatag tgtaaaagtg gcgagttcaa ataaagaaat atatttacag
     3781 ccggcaactg ctgtagaaga accctatttt gatgaatcgc taacatatgc aaatagtgtt
     3841 ttagacttgg aattaatgcc cgataattct gccgcttacc ttacaatagc ttcatttaat
     3901 tattatagat ggaacgactt cgatgtattt aaaaatttta ttgatagcag ttttcaggta
     3961 attaataaaa ataagataga taacttaatt attgatttac gatttaatgg tggcgggtcg
     4021 caatcggcca gtatacatct tttgcgatat ttgcttgatg agccttttac atattattca
     4081 aatgcacaat ttgaaggaaa acaaggcaaa attgaagggg aggaattaat ttatccattt
     4141 gaaaacagat ttaaaggtaa aagttatttc attattgatg gacatgggaa ttctacaact
     4201 ggacatttta tgtcaatagt gaaacatttt gatgtaggca ccatcgtggg cgaagagtta
     4261 ggttccaatc agttttgttc tgcgggtatg actacctgcc gactgtcaaa tactaaactt
     4321 gtttattacg tggcaaataa cactcatgaa t
//
LOCUS       DAORKW010000294         4339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_965_length_4339_cov_5.397059, whole genome shotgun sequence.
ACCESSION   DAORKW010000294 DAORKW010000000
VERSION     DAORKW010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_965_length_4339_cov_5.397059"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLB46_11100"
     CDS             <1..226
                     /locus_tag="PLB46_11100"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HRF77111.1"
                     /translation="IYFDGEMFYHKTNAVLRIARKLRFPFPLFYGLIVFPPFIRNAVY
                     DFIAKYRYVWFGKRESCMIPQPQWKQRFLG"
     gene            275..2182
                     /locus_tag="PLB46_11105"
     CDS             275..2182
                     /locus_tag="PLB46_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF77112.1"
                     /translation="MRSVLLFTILGVLMLLLTYCNNNNKVVVNDSPWLNQNDTVQYVG
                     MATCKLCHADQHTTFIHTGMGKSFDYATRSKSAALFDKHAIVFDSINNYYYQPFWRND
                     TLMIKEYRLDGADTIHQRTESIQYIIGSGQHTNSHMLNFNGYVFQAPITFYTQKQKWD
                     LAPGMELGFNSRFSRIITAECMTCHNGLPKMVAGSVNKYTKVPSGIDCERCHGPGSLH
                     VARVSNNILVDTATGIDYSIVNPRHLSVELQNQLCMRCHLQGVNVLNEGASFFDFKPG
                     DKINEHWNIFLPKFDGKNDKFLMASQADRMVQSKCYIETQQLSCITCHNPHISVKETP
                     AAQFNAACINCHQPNKNDCTETIAVRLTNNDNCSACHIPKSGAIDIPHVSISDHKIQI
                     PGEAVKMGDGKFIGLVSLTTDKPDALLMARGYLHYFESFNSDRALLDSVKYYLDKTSE
                     KNDSYSAVLIHYYYLQQDFNKIISASKAFKKITTLDAWTAYRIGEAFLNTQSYTEAAK
                     YFEQAVNEEKYNLDYNFKLATAKIFSGKAEEAEKLLRFIVTENPTYHMAWNNLGVLQA
                     GKGNYQEAEKSFAKAIQLDPDYVLARIKLAELYIATRQRTKAREAVSYLEKYYPDLQE
                     LPKLTLQLRSI"
     gene            2202..3161
                     /locus_tag="PLB46_11110"
     CDS             2202..3161
                     /locus_tag="PLB46_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRF77113.1"
                     /translation="MRINIIPKSFQRKIGVKYRDFIAFTQRTSFPGFDKIPIYDVLTF
                     FRQEIKRDQLPVRASAISFNFLLAVFPSIIFLFTLIPYIPISGLDVSIHKFFKDVLPE
                     SGYAFLESTINSITTIKRSGLLSIGFLLAFYFSTNGVRMILLTFNKNHPNYAKRNFWR
                     NRFASFRLTLYLFVLFIASIILIITGDIFVEWFGGLINWEDTTGKIFLTLFQYVVILL
                     LFFLGISLIYYYGPAVKHRWRFVTPGATFATIFSILTSLGFGYLANNFISYNEVYGSI
                     GTLIVLMIWINLNSLVLLVGFEINNAVDVNRILRQRADDVEEK"
     gene            3345..3704
                     /locus_tag="PLB46_11115"
     CDS             3345..3704
                     /locus_tag="PLB46_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRF77114.1"
                     /translation="MILYYLGTCSTCARIMKELKVNKYNFQLQDIKTSPITGAQLDAM
                     KKLAGSYEALFSRVALKYRALGLNEMTLTEKDYRKYILEEYTFLKRPVFIINNAIYIG
                     NSPKNIQAVAEALKSLK"
     gene            3811..>4339
                     /locus_tag="PLB46_11120"
     CDS             3811..>4339
                     /locus_tag="PLB46_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77115.1"
                     /translation="MLKIFTPLLISSTLLTHQLAFSASNPLELPFSPALAAPMLLDGE
                     DCDHAVTVTEGTYTAPNPDYWYVYTAEATGTYLLSTCFPENTCDTRMYLYSYCIGLIP
                     SELAEGTLSYNDDYCGTRSQITTLLTEGDVIYIRIGDAETACAGEEIIWSLTYAGEPE
                     GCTDTYACNWSPIAMI"
BASE COUNT         1356 a          756 c          827 g         1400 t
ORIGIN      
        1 aatttatttt gatggtgaga tgttttacca taaaacaaat gctgttttac gcatagcacg
       61 taagttgcgc ttcccatttc cactgtttta tgggttaatt gtttttccac catttatacg
      121 caatgctgtt tatgacttta tagctaaata tcggtatgtc tggtttggaa aaagggagag
      181 ttgtatgata cctcaaccac agtggaaaca gcgctttctg ggataagcaa ctttcaattt
      241 gcgccatcgt aaataatcgg ctatttttac acaaatgcgc tccgttttac tttttaccat
      301 attaggtgtt ttaatgttat tacttactta ttgtaataat aacaataagg tagtggtgaa
      361 cgattctccc tggctgaatc aaaacgatac cgtgcagtat gtaggaatgg caacatgtaa
      421 actttgtcat gctgaccagc atacaacttt cattcatacc ggtatgggta aaagtttcga
      481 ctatgctacg cgaagtaaaa gtgccgcatt gtttgataaa cacgctattg tatttgattc
      541 catcaacaat tattattacc aaccattttg gcgtaacgat acgttaatga ttaaagaata
      601 tcgtttagat ggagccgata ctatccatca acgaacagaa tcgattcagt atattattgg
      661 cagtggtcag catactaact cacatatgct aaattttaac gggtatgtat ttcaggcacc
      721 aattactttt tatacccaaa aacaaaaatg ggatttggca cccggaatgg agttaggatt
      781 taattcacgg ttttcccgaa ttattactgc tgaatgtatg acatgtcata atggtttgcc
      841 gaaaatggtt gcaggttcgg tgaacaaata tactaaagtg ccaagtggta ttgattgtga
      901 aagatgtcat ggtcccggaa gtttacatgt tgcgcgtgta agtaataata tattagtgga
      961 tacagcaaca ggaatagatt acagtattgt taacccacgt catttatcgg tggaattaca
     1021 aaaccaatta tgcatgcgtt gccatcttca gggcgtaaac gttttgaatg aaggggcttc
     1081 attttttgac tttaaacccg gtgacaaaat caacgagcat tggaatatat ttctaccgaa
     1141 atttgatgga aaaaatgata aatttttgat ggcatcacag gcagatagaa tggtgcaatc
     1201 gaaatgttat atcgaaacac aacaactgag ttgtataact tgtcataatc cgcatatatc
     1261 tgttaaagaa acgccggcgg ctcaatttaa tgctgcttgt atcaattgtc atcaacccaa
     1321 taaaaatgat tgcaccgaaa caattgcagt gcggttaacg aacaacgaca attgtagtgc
     1381 atgccatatt cctaaatcag gagccataga tattccgcat gtatccattt ctgaccataa
     1441 aattcaaatc cccggtgaag cagtaaaaat gggtgatggt aaatttatcg gattggttag
     1501 tttaactaca gacaaaccgg atgcactttt aatggcccga ggttatttac attatttcga
     1561 atcatttaat agcgacagag ctttgttaga tagcgtaaaa tattatttgg ataaaacttc
     1621 cgaaaaaaat gacagttata gcgcagtgct gatacactac tattatttac aacaagattt
     1681 caataagatt atttcagcga gtaaagcatt taaaaaaata actacattgg atgcttggac
     1741 agcctatcgt attggagaag catttttaaa cacacaatcc tatactgaag ctgcaaagta
     1801 tttcgagcaa gctgttaatg aggaaaaata taatcttgat tataatttta aattggcaac
     1861 agcaaaaatt tttagtggaa aggctgagga agctgaaaaa ttgcttcggt ttattgtaac
     1921 agaaaatccc acttaccata tggcttggaa taacctgggt gtattacaag caggtaaagg
     1981 caattatcag gaggcagaaa aaagttttgc aaaggctatt cagttggacc cggactatgt
     2041 tttggcacga atcaagcttg ccgagttgta tattgccact cgccagcgca ctaaagcaag
     2101 agaagcagtc agctatctgg agaaatatta ccccgatttg caggagttgc ccaagctaac
     2161 cctgcaactt cgcagcatat agttaacttt gcacccgaca aatgcgtatc aatattattc
     2221 ccaaatcgtt tcagcggaaa attggcgtta agtatcgcga ctttattgcg tttacacagc
     2281 gtaccagttt cccggggttt gataagattc ccatttatga tgtgctcact tttttcaggc
     2341 aggaaatcaa acgcgatcag ttacctgtga gagcctcggc tatatccttc aatttcctgt
     2401 tggcagtatt cccttctatt atctttttat tcaccttaat cccatatatt cctattagcg
     2461 ggttagatgt aagtattcat aagtttttta aagatgtatt accggaaagt ggttatgcat
     2521 ttttggagtc taccattaat agtataacta ccattaaacg aagcggatta ttatctatcg
     2581 gttttttatt ggcattttat ttctctacca atggtgtgcg catgatttta ttgacgttta
     2641 ataaaaatca tccgaattat gccaaacgca atttttggag aaacagattt gcttcttttc
     2701 gattgacttt atatttgttt gttttattca ttgcctcaat tattttaatt attaccggtg
     2761 atatttttgt ggaatggttt ggcggtttaa ttaattggga ggatacaaca ggaaaaatat
     2821 ttttaaccct atttcaatat gttgttattt tgttattgtt ttttctgggc atatcattaa
     2881 tttattatta cggcccggca gttaaacacc gctggcgttt tgttacgccg ggcgcaacct
     2941 tcgctactat tttttcgata cttacatcct tgggttttgg atatttagca aataatttca
     3001 ttagctataa cgaagtgtat ggttcaattg gaaccttgat tgtgttgatg atttggatta
     3061 acttaaattc attagtatta ctggtaggat ttgaaataaa taatgccgtt gatgtaaata
     3121 gaattctgcg tcagcgagcc gatgacgtag aagaaaagtg atagtagtaa aatttgattt
     3181 aacacgcata gccttctcag tattccatcg cgaagcatat taaagcaatt acataatcgg
     3241 taattggtaa catgtataaa ttgtttctga tattatttta agcaacatca ttttataaca
     3301 tacacctcaa aaacagctaa atttgtactt ataaataaaa tgatatgata ttgtattatc
     3361 tcgggacgtg ttctacttgt gcacgtataa tgaaagaact aaaagtaaat aagtataatt
     3421 ttcagcttca agatattaaa acatcaccaa taacaggagc acaattggat gccatgaaaa
     3481 aattggcagg ttcatacgag gcattgttta gtcgcgttgc attaaaatat cgggccttgg
     3541 gattaaatga aatgaccttg actgaaaaag attatcggaa atacatcctc gaagaatata
     3601 cctttttaaa acgccctgtt tttatcatta acaatgccat atatatcggt aactcaccca
     3661 aaaacatcca ggctgttgct gaggcgctta aatcattgaa ataaattcaa aaaatagcaa
     3721 aattgtgact gctgaatacg gatggaattc aagcctattt tgcatttctg aagaaactta
     3781 ttatttttaa catgtataaa cttaataaac atgttgaaaa tttttactcc tctactcatt
     3841 tcctcaactt tgttaacaca ccaactggca ttttcggcta gtaatccttt ggaacttccc
     3901 ttttcaccag cacttgcagc acccatgctt ttggatgggg aagactgtga ccatgctgtt
     3961 accgtaacgg aaggcactta cacagcgccg aatccggact attggtatgt gtatacggct
     4021 gaagctacag gcacctacct tttgagcact tgttttccag aaaacacttg tgataccagg
     4081 atgtatttgt acagctattg cataggtttg attccttctg agttagctga gggtacgctt
     4141 agttacaatg atgactattg tggaacaagg tcacaaatta caaccttact taccgagggc
     4201 gatgttattt atatacgaat tggtgacgcg gaaactgcgt gtgccggaga agaaattata
     4261 tggtcattaa cctatgcggg tgaaccggaa ggttgcaccg atacttatgc ctgtaactgg
     4321 tcaccaattg cgatgattg
//
LOCUS       DAORKW010000295         4336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_968_length_4336_cov_4.525111, whole genome shotgun sequence.
ACCESSION   DAORKW010000295 DAORKW010000000
VERSION     DAORKW010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_968_length_4336_cov_4.525111"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..525
                     /locus_tag="PLB46_11125"
     CDS             67..525
                     /locus_tag="PLB46_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HRF77116.1"
                     /translation="MENQKKIDALNKLITINNDRIEGYETASKETEETDLKSLFAHLM
                     ATSQKCKRELEAEVLALGGEPSEGTKDSGKFFRAWMDVKAALTGKDRKAILNSCEFGE
                     DAAIKTYENVMEDDAKHFDSQIENLINAQFSLLVADHNTVKSLRDALASA"
     gene            982..4002
                     /locus_tag="PLB46_11130"
     CDS             982..4002
                     /locus_tag="PLB46_11130"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRF77117.1"
                     /translation="MLFKPTLTSISIVIISICNLEAQVVINEGSNKNYIYGVDEEGDN
                     EDWIELYNAGGSAVDLSGYHLSDKSSDPELWPLTGIVIQPNNYLQIFCSEKNRYATAP
                     FFNAVNDLDYSPFAGWNTHHFTSDFEWDGSSNLIINICSYSNVGYTENAIFKQTATDY
                     VSTVGAFNDGSDASCSATIGAMYYQRPNMQINGITIDEGTIENGNTDYPAPYGNWYWS
                     ARHQILIRADELLAAGISAGPINSIGFEVVSTNEIVYTYIDISIFATAQDELTEVLSP
                     VSGFRNHTNFKIDAEGETVYLFDESDAITSSLEILSPRADVAIGRSPDNSDNIEWLSP
                     TPGATNNTANVFTDELLPPVLSVATGIVNSSFDLEITNPNAGIIETKLVYTLDGSEPT
                     TTSDTYTGTPIAINNSTVIRAKIFPIVDNNYLPSFDTYGTYLFNIDHSTPILLLTTDN
                     NNLYGADGIFDNYNSDWVKAAHVTYLTKETGHPTLFETRTAIRMDGGAGGSRAYPQHS
                     FRLSFDHAALGEETINEQLIPNIPFRNKYSDVYLRNGSNQWLTFPHKDACQVSMMSKG
                     TNNYYSAMEPVSVYINGEYFGLYELREKFNTEYFDVREDVDKDSIEILSLSYFYNLIL
                     RALEGDVNNFYNDYELFNALDPVDTNYVSLADKYFDLAHYTDYIIGESWMGNTDWPGN
                     NIKIYRSNTTNFRWRFALIDLELSLNPNGWTTCPYNHIRYMKDQSTDNPFINIWLQSI
                     QNETYLNSFINRYADLMNTSYLADTLVAASQAFYDKMVLEMPNEYARWGDPFNVEGQM
                     EDFENNHEVFQQQLACRNEQVRGDLVNEFDLEKEVTVNLAIQPDSSGSILLNTIHPTI
                     YPWSGIYFDGVPIQLTAVADSGYVFVNWLPNPFITDTLNPVLETNVDLDSTTFTAVFK
                     LIPPPPDGETIDFTLFPSPSSGLITIAHNNSTLAEKATFQIFDLQGRLLSSGNIGQDA
                     KTTTIDVASLSSALYYVRIMNGAEIMDTLSFVRL"
     gene            complement(4047..>4336)
                     /locus_tag="PLB46_11135"
     CDS             complement(4047..>4336)
                     /locus_tag="PLB46_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF77118.1"
                     /translation="AAEDFLIYADCYKHITKIGSPTYGSTGQPYLFDLPGGGRARICT
                     KNDTYPDGRQFIGYGIVPDIAIKQSLQDYLNNNDVVLQKAIEVLNTQLKSK"
BASE COUNT         1426 a          695 c          816 g         1399 t
ORIGIN      
        1 aaaaggataa gtcaataatg cgatattgaa aaactattca attaataatt aaaaacaaat
       61 ataaaaatgg aaaatcaaaa aaaaattgat gcgttgaata aacttatcac tatcaacaat
      121 gacagaattg aagggtatga aaccgcttct aaagaaactg aagaaactga tctcaaatct
      181 ttatttgcac atttaatggc taccagtcaa aaatgtaaac gggaactgga agctgaagta
      241 ttagcactag gtggtgaacc atcagaggga actaaagatt caggtaaatt ttttcgcgca
      301 tggatggacg tgaaagctgc attaaccggt aaagaccgca aagcaatttt gaattcttgt
      361 gaatttgggg aagatgctgc gatcaagact tatgaaaatg taatggagga tgatgcaaaa
      421 cattttgaca gccaaattga aaacttgata aacgctcagt ttagtttatt agttgctgat
      481 cacaataccg taaaatcgtt acgtgatgca ttagcaagtg cctgatgcaa acatgaaact
      541 gttttgcttg tacatcatct aaacgctaat tacctgtcat ttaaatcagg tatttctata
      601 gtaatagtaa tgtcaccaat tgataataca gtaatgtatt gttggttggt gttttttttt
      661 aatagtgttt gatcaatcaa atcccttgat tatgtaatcg aatgtttaag ccgatgttca
      721 acaaattcaa ttgaatttac ggaatgtata aaaattgcag atttgtgaca attacatctc
      781 caccaacata atgtttaatt tagaccttaa aaaatgctat aaccttttat tttacggggt
      841 tttaagtgat atttcaggtg ttttgattga cattatgata aatggggggc taaaccaaag
      901 tttacaattt tataatttta aattaatttt gggagaacat gctaaagcaa actgcatagc
      961 ataaccacgc aatccactcc tatgttattt aaacctaccc tgactagtat ttctatagta
     1021 attatttcta tttgtaacct tgaagcccag gtggtcatta atgaaggttc taataaaaat
     1081 tatatatatg gtgttgacga agaaggtgat aatgaggatt ggatagaatt atataatgcc
     1141 ggtggcagtg ctgttgactt gtccggttac catttaagtg ataaatcttc tgatccggaa
     1201 ttatggccac taaccggcat agtaattcag cccaacaatt acctacaaat attttgcagt
     1261 gaaaaaaacc gatatgcaac agcgcctttt tttaatgcgg taaatgattt agactattca
     1321 ccatttgcag gatggaatac tcatcatttt acatccgact ttgaatggga tggaagtagc
     1381 aacctgataa tcaatatttg ttcatatagt aatgtaggtt atacagaaaa tgccattttc
     1441 aaacaaactg ctactgatta tgtttcaaca gttggtgctt ttaacgatgg cagtgatgca
     1501 tcatgttctg caacaattgg cgctatgtat tatcagcgcc ccaacatgca aataaatgga
     1561 attacgattg atgaaggaac tatagaaaat ggtaataccg attatcctgc accttatggc
     1621 aactggtatt ggagtgcgag acatcagata ttaattcgtg ctgatgaatt acttgctgcc
     1681 gggataagcg cagggccaat caactccatt ggttttgagg ttgtttcaac taatgaaatc
     1741 gtgtatactt acattgatat atcaattttt gctacagctc aagatgaatt aactgaagta
     1801 ttgtcaccgg taagtggttt ccgcaaccat acaaatttta aaattgatgc tgaaggtgaa
     1861 actgtttatt tatttgatga aagtgacgca ataactagtt cgctggaaat tttatctccc
     1921 agagccgatg ttgccattgg acgttcaccg gataattctg ataatattga atggttgtcg
     1981 ccaacacccg gagcaacaaa taatactgct aatgttttta cagatgaatt attgcctccg
     2041 gtattatcag tggcaacagg tatagtaaac agcagtttcg accttgaaat taccaatcct
     2101 aatgctggca tcattgaaac caaattagta tatactttag atggcagcga accaactacc
     2161 acttctgata cgtataccgg aacaccaatt gcgattaata attcaactgt aatcagggcg
     2221 aaaatatttc caattgtaga taataattat ttgcccagct ttgatacgta tggcacctat
     2281 ttatttaaca ttgatcatag cacgccaatt ttattattaa cgactgacaa taacaattta
     2341 tatggtgctg atggcatatt cgataattat aattctgatt gggtaaaagc tgcgcatgtt
     2401 acctatctga ctaaagaaac cggacatcca acgttgtttg aaacacgcac tgctattcgc
     2461 atggatggag gagcaggtgg aagcagagca tatcctcaac attcattccg tttatcattt
     2521 gatcatgcag cattaggcga agaaacaatt aatgaacaac ttattcctaa tataccgttt
     2581 cgaaataaat acagtgatgt ttacctgcgt aacggcagta atcaatggct aacatttcca
     2641 cataaagatg cttgtcaggt aagtatgatg agtaaaggaa ccaataatta ttattcagct
     2701 atggaaccag taagcgtata tataaatggc gaatattttg ggttatatga attgcgtgaa
     2761 aaatttaata ctgaatattt tgatgttcgt gaagatgtag ataaagattc cattgaaatt
     2821 ttatctctca gttattttta caatttgatt ttgcgtgcac tggaaggaga tgtaaataat
     2881 ttttataacg attacgaatt atttaatgct ttggatcccg ttgatacgaa ttatgtatca
     2941 cttgcagaca aatatttcga tttggcacat tatacagatt atattattgg tgaatcctgg
     3001 atgggaaata ccgattggcc cggtaataat attaaaatat atcgctccaa tacaaccaat
     3061 ttcagatggc gatttgcact aattgattta gaattatcgc tcaatccgaa tggttggaca
     3121 acctgtccat ataatcatat tcgatatatg aaggaccaaa gtacagacaa tccatttatt
     3181 aatatctggt tgcaaagtat tcaaaatgaa acttatctca atagttttat taatcgttat
     3241 gcggatttaa tgaataccag ttatttagca gatacgttgg tagcggcttc gcaagccttt
     3301 tatgataaaa tggtgcttga aatgcctaat gaatatgcac gttggggcga tccttttaat
     3361 gtggaaggtc aaatggaaga ttttgaaaat aatcatgaag tttttcaaca acaattagct
     3421 tgtcgaaatg aacaggttcg tggtgattta gtgaatgaat ttgatttgga gaaagaagta
     3481 acagtaaacc tcgctattca accggactca agtggaagca ttttgttaaa taccattcat
     3541 ccaaccattt atccttggtc aggtatatat tttgatggtg tgccaattca attaactgca
     3601 gttgccgatt cgggatatgt atttgttaac tggttaccaa atccatttat tacagatact
     3661 ttgaatcctg ttttagaaac taatgtagac ttagactcca ccacatttac tgctgtgttt
     3721 aaattaattc caccaccacc tgatggagaa actattgatt ttacattatt tccttcacca
     3781 agttctgggt taattactat tgcacacaat aacagcacac tggctgaaaa ggcaacattt
     3841 caaatttttg atttgcaagg cagattatta tcatcaggta atataggaca agatgctaaa
     3901 acaactacaa ttgatgttgc atcacttagt tctgcacttt attatgtgag aattatgaat
     3961 ggagcagaaa ttatggatac tttgtcgttt gtgaggttgt gatttttttt ggccgggaag
     4021 gcgggaagac gggaagttac ggggagttat tttgatttta gttgagtgtt gagtacttct
     4081 atggcttttt gtaatactac atcgttatta tttaaatagt cctgtagtga ttgtttaatt
     4141 gctatgtccg gaactatacc gtaaccgata aattgtctgc catcaggata agtatcgttt
     4201 ttggtgcata tacgcgcacg accacctccc ggtaaatcaa ataaataggg ttgtccggtg
     4261 ctgccatacg ttggagaacc aatttttgta atatgtttat aacaatcggc ataaattaaa
     4321 aaatcttcgg cagctg
//
LOCUS       DAORKW010000296         4334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_969_length_4334_cov_3.894134, whole genome shotgun sequence.
ACCESSION   DAORKW010000296 DAORKW010000000
VERSION     DAORKW010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_969_length_4334_cov_3.894134"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PLB46_11140"
     CDS             complement(<1..929)
                     /locus_tag="PLB46_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRF77119.1"
                     /translation="MYQRRFKTYVFSFLRHDLKSSITVFFVALPLCLGISLGCGAPLY
                     AGLIAGIIGGIVVPVISKSSLSVSGPAAGLTTICAAIILASASIDLFFIAVMLAGVFQ
                     IVLGVFKLGGFTQFIPSAVIKGMLGAIGILLIAKQIPFMVGYDQPDFWTNELFNILTF
                     NHGFEHLGSLYQHSSGGAIITAVLSLLLLIFWKRNLSKKIPYLPSSFIIVIFGSLVAF
                     LIGNFFPVLALKDSQYVNIPKNVFADFHLTNLNLIVTDATIWKNAIVICFVASLETLL
                     SIAAIDKLDPYNRITPQNRELVAQGTANFLSGW"
     gene            complement(1017..1379)
                     /locus_tag="PLB46_11145"
     CDS             complement(1017..1379)
                     /locus_tag="PLB46_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77120.1"
                     /translation="MGNSIHMLNKITPISVVLLIIGFVSCTAGKQATSTALTYTTNVK
                     PIIDANCATTCHNAGRPAGGIDLTTYEKVKEQSLNGKLISAIQHADGAKPMPKKAPKM
                     DDVTIQTIVNWANSGAAL"
     gene            complement(1440..2738)
                     /locus_tag="PLB46_11150"
     CDS             complement(1440..2738)
                     /locus_tag="PLB46_11150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF77121.1"
                     /translation="MEPFPVLIAYKRFTLIMSATKTPVFTGYQWFIIAILAFLQFTIV
                     LDFMVLSPLGAILMPELSITPGQFSHLVSAYAYSACISGILASGFADKFDRKKMLLFF
                     YAGFLAGTVFCGLAPDYTSLLTARIITGLFGGVMGAVVFAITTDLFEMQVRGRVMGFV
                     QMAFAASQVLGLPIGLQLANSMNWHAPFMLIAGIGAAVFVVILIKMKPVTAHLAIQRK
                     VSPFRHLWMTLSRKRYLTGFAATVLLATGGFMLMPFGSAFTVNNLGIDLDHIPLIYMF
                     TGAATLIAGPIIGKFSDRVGKYNMFVAGSILSMILVLIYCNLGITPLWVAIVVNIVLF
                     IGITARIISAQALMSGVPEPADRGAFMGLNSSIQQLAGGLAASLAGLIVVQEDSGKLS
                     HYPILGYVVTVAMFITMIMMFVINKMVVSDNKLPNAIPAK"
     gene            2982..3674
                     /locus_tag="PLB46_11155"
     CDS             2982..3674
                     /locus_tag="PLB46_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRF77122.1"
                     /translation="MLLQTLLQIPGASPVTTPTTTTTPAESLSLIDLTLAGGIVMIPI
                     GILFILAIFLYFERYLTIRKAAKGDPKFMLNIRDYISGGNLEAAKMLCKNTDTPIARM
                     IEKGIMRLGSPLKNIEVAVENVGKLELYKMEKNLAAIAMISGVGPMLGFLGTVTGMIQ
                     AFYIMSQSDQISPKVLSGGIYEAMVTTVAGLIVGIVANVAYNSLIALVEKMIYKMEAT
                     TVEFVDLLQEPA"
     gene            3702..4097
                     /locus_tag="PLB46_11160"
     CDS             3702..4097
                     /locus_tag="PLB46_11160"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRF77123.1"
                     /translation="MGLRSRNKRLAEFQLASLTDIIFLLLIFFVLTSSIVAPNALNIL
                     LPNSDSQTQATQSVSISIDKDLKYYLNDEEIDPAWLETMLPSKLAGQTDPTVVLSADK
                     SVPVEYVVGVMNIASNMKIKMVLATTPKK"
     gene            4154..>4334
                     /locus_tag="PLB46_11165"
     CDS             4154..>4334
                     /locus_tag="PLB46_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77124.1"
                     /translation="MKNWEEFEKANKRRSGFITTGIVAAVACIMFLFGFKPPDPPLEP
                     EGLLIDFGTDATGSGS"
BASE COUNT         1512 a          796 c          770 g         1256 t
ORIGIN      
        1 atccaaccac tcaggaaatt tgctgtaccc tgagcaacga gttcacggtt ttgaggcgta
       61 attctattat agggatccag cttatcaata gcggcaatac taagtaaagt ttccagtgag
      121 gctacaaaac aaattacgat ggcatttttc caaatagtag catcggtaac tataaggttt
      181 aagttggtca aatgaaaatc agcaaaaaca ttttttggaa tatttacata ttgactatct
      241 tttaaagcta aaacgggaaa aaaattacca attaaaaaag caaccaaact tccgaatatt
      301 acaataataa atgaagaagg taaatacgga atttttttag ataaatttcg tttccagaaa
      361 attaataata acaaactcaa aaccgcagtg ataatggcac ctccggagga atgctgatac
      421 aatgaaccta agtgttcaaa accatgatta aaagtgagaa tattaaacaa ttcgttcgtc
      481 cagaaatcag gctgatcata tcccaccata aatggtattt gtttggcaat gagtaaaatt
      541 ccaattgcac ctaacatccc tttaataact gctgaaggga taaattgtgt gaaaccacct
      601 aatttaaaaa cacctaaaac aatttgaaaa actccggcaa gcattactgc aataaaaaag
      661 aggtcgatgg atgcagatgc aagaattata gctgcacaaa ttgttgttaa accggctgca
      721 gggccactca cgcttagtga agatttactt ataacaggaa caactatacc accaataatc
      781 cctgctatta aaccggcata caaaggcgca ccacatccta atgaaatgcc aagacatagc
      841 ggtaacgcaa caaaaaaaac agtaatactc gattttaagt cgtggcgaag aaaagaaaat
      901 acatacgttt taaatctgcg ttgatacata tgcataaata aacctcaaag gtgagagtaa
      961 taaatagcaa tatgctattt acttatgcat atagtaattg tgcattgcgg agtaacttat
     1021 aatgcagcac ccgaattagc ccaattcaca atggtttgaa tagtaacatc atccattttc
     1081 ggggcttttt taggcatagg tttggcacca tcagcatgtt ggatggcact aatcaacttg
     1141 ccattcaggc tttgttcttt caccttttcg taagtagtta aatcaatacc tcctgcaggg
     1201 cggccggcgt tgtgacatgt ggttgcgcaa ttggcatcaa ttattggttt tacatttgtg
     1261 gtataggtta atgcggttga agtagcctgt tttcctgcag tacaagaaac aaaacctatt
     1321 attaaaagca ctacagaaat aggggtaatt ttattgagca tgtggattga atttcccata
     1381 aaattataca actcaattaa ttttcacaac tatgggttaa ccgcatactt gatgttctcc
     1441 tattttgcag gaatagcatt aggcaattta ttatcgctta ccaccatttt attaatcaca
     1501 aacatcataa tcatggttat aaacatggca actgtaacta catatcctaa tataggataa
     1561 tggctcaatt tcccggaatc ttcctgcaca acaattaaac cggccaatga tgcagccagg
     1621 cctccggcta attgctgaat actggaattt aatcccataa aagcccctct gtctgctggc
     1681 tccggaactc ccgacataag tgcctgtgca gaaatgatac gagcagtgat accaataaat
     1741 aaaacgatat taacaacaat tgcaacccat agaggtgtta tacctaaatt acaatatatt
     1801 aataccaaaa tcatagataa aattgagccg gcaacaaaca tattatactt tcctactctg
     1861 tccgaaaact taccaataat tggtcccgca attaaagtag ccgcaccggt aaacatatat
     1921 attaaaggaa tatgatcgag gtctattcct aaattattaa ctgtaaatgc gctaccaaac
     1981 ggcatcaaca taaatccgcc cgttgctaat aataccgtag cggcaaaacc ggttaagtac
     2041 cgttttctcg ataatgtcat ccacaaatgc ctgaatggcg aaactttgcg ttgtatggct
     2101 aaatgtgctg taacaggttt cattttaatc aatattacca caaacactgc agcgccaatt
     2161 ccagcaatca acataaatgg agcatgccag ttcatggaat ttgccagttg tagcccaatg
     2221 ggtaatccaa gtacctggct ggctgcaaat gccatctgaa caaaacccat aacgcgcccc
     2281 cttacctgca tttcaaataa atcggttgta atggcaaaaa ctacagctcc cataacaccg
     2341 ccaaataaac cggtaataat acgtgcagtt agtaaagaag tatagtccgg tgctaatcca
     2401 caaaatacag tacctgctaa aaacccggca taaaaaaata ataacatttt ttttctgtcg
     2461 aatttatcag caaacccgga agctagaata ccactgatac aagcagagta ggcatatgcc
     2521 gaaactaaat gactaaactg tcccggagtg atagataatt ctggcatcaa aatggcacct
     2581 aatggtgaaa gcaccataaa atcgagcaca atagtaaatt gtaaaaatgc aagaatagca
     2641 atgataaacc attgatatcc ggtaaataca ggcgttttgg tagctgacat aattaaagta
     2701 aaccgtttat aagcaattaa aaccggaaat ggttccaaag cttaaatttg gcggctgcaa
     2761 gattagtcat attaatattg attttgtaat aaattgcggt aaaacgcctt tttaaatagg
     2821 taagtgaagt aaaaatgaaa ctaaaatttt gtccattata tcctacaaat acaacctttt
     2881 acgtttaaat ataaggctgt aaacctcagt catcatgata attttgttcc ttgataaata
     2941 ccataatttt ttatttgaac gtttaatata aaattttcta aatgctgcta caaaccttat
     3001 tacaaatacc gggtgcatca cctgtaacaa cgccgactac tacaaccact ccggctgaaa
     3061 gtttatcatt aattgattta acccttgcag gtggaattgt aatgatacca attggtatat
     3121 tatttattct ggccatattt ttatattttg aacgatattt aactattcgc aaagcagcga
     3181 aaggtgatcc gaaattcatg ttgaatattc gcgattatat ttcaggcgga aatttagaag
     3241 ctgcaaaaat gttatgcaaa aatactgata cgcctattgc acgtatgatt gaaaaaggta
     3301 ttatgcgttt aggctctcca cttaagaata ttgaagtggc tgtagaaaac gttggtaaac
     3361 tggaattata taaaatggaa aaaaatctgg ctgctattgc catgatttcg ggtgttggtc
     3421 caatgctcgg atttttggga acagttaccg gtatgattca ggcattttat atcatgagcc
     3481 aaagcgacca aattagtccg aaagtattgt ctggcggtat ttacgaagcc atggttacta
     3541 ccgttgccgg tttaattgtt ggtattgttg ccaacgttgc ctacaatagt ttaatagcgc
     3601 tggtagaaaa aatgatatat aaaatggaag ccactactgt tgaatttgtg gacttattgc
     3661 aggaacctgc ataatttaat ttcatcagta aataacttaa aatgggatta cgatcaagaa
     3721 ataaacgttt agcagaattt cagttggcat cgctcactga tattatattt ttattgttga
     3781 tattttttgt attaacatcc agtattgttg cgccaaatgc attaaatatt ttattaccaa
     3841 attccgattc acaaacacag gcaacacaaa gtgtgagtat atctattgat aaggatttaa
     3901 aatattattt aaatgatgag gaaattgatc ctgcatggtt agaaacaatg ttgccatcaa
     3961 aattagcagg ccaaaccgac cctacagttg tactttcggc agataaatct gtcccggttg
     4021 aatacgtagt tggggtaatg aatattgcca gtaacatgaa aatcaaaatg gttttggcaa
     4081 ctacgccaaa aaaataggaa tgagatttgt ggaataacat ggaattgggc atctaattat
     4141 aaaaccaatc attatgaaaa attgggaaga atttgaaaaa gcaaataagc gcagaagtgg
     4201 tttcataact acgggaatcg ttgctgccgt tgcatgtatt atgtttttat ttggctttaa
     4261 accacctgat ccgcctttag aacctgaagg tttattaatt gacttcggaa cagatgccac
     4321 cggttccggt tctt
//
LOCUS       DAORKW010000297         4330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_970_length_4330_cov_4.672281, whole genome shotgun sequence.
ACCESSION   DAORKW010000297 DAORKW010000000
VERSION     DAORKW010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_970_length_4330_cov_4.672281"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..301
                     /locus_tag="PLB46_11170"
     CDS             74..301
                     /locus_tag="PLB46_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77125.1"
                     /translation="MSNQVLQTSEELKILTENLNNNNTSVAGVLLNDESAAVNLKQSL
                     INLESSSQKLNENMEALQHNFLFRRYFKNKD"
     gene            complement(407..664)
                     /locus_tag="PLB46_11175"
     CDS             complement(407..664)
                     /locus_tag="PLB46_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HRF77126.1"
                     /translation="MKCPLCIDKELVMSDRQGVEIDYCPACRGVWLDRGELDKLIERS
                     GERFINKSKSAHPQNEINDYKHQQHPKQYKKKSIWGEILDF"
     gene            complement(798..1019)
                     /locus_tag="PLB46_11180"
     CDS             complement(798..1019)
                     /locus_tag="PLB46_11180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77127.1"
                     /translation="MYPNPAVVAQHIDFEDTPVNGSLMIIDFAGKVVFAQIVDDYSAI
                     IDLQEIPAEIDVLEVANGDEVQRSEFIKL"
     gene            complement(1212..2231)
                     /locus_tag="PLB46_11185"
     CDS             complement(1212..2231)
                     /locus_tag="PLB46_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77128.1"
                     /translation="MKQLQLTIALCLVIFGSIQNAHGQFDSYPIDGYTELSVKKATEF
                     AERKLIVTTLEYDDDIVKKLDKNKDPEKLATYKATIKEFNADIKKMVDSLWTLNKSIK
                     YMSYEDIKDIKGDDKSQYVVIIMERMEEKTSTGTSGKTYVIGNYDITFDNSAIGGIQK
                     TNKIRSKSSHFTVMRVALLENLQFPNQMTIPMGHIVPQSTDIAFGLTYIDWYIRSKMA
                     DISDKDMMIQINEKSKELATLTLLISEEDAKDDDIMGKGSKVYPFKSQIVTKEELEAH
                     ILAKTPGYAYVNYGHNSACAVKVDNRTILRVPKPSEVTLVSTENTIAKMGISVQKLIE
                     KDEEE"
     gene            complement(2402..3493)
                     /locus_tag="PLB46_11190"
     CDS             complement(2402..3493)
                     /locus_tag="PLB46_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77129.1"
                     /translation="MKTKLLSYSGAASAFLLASINELKSQIIYTDIDPDVYISGDPVD
                     YYMDLNLDGEFEVHFDHIGNFYGSVGCLGFVVPNYSYCFISYQYDSLAMYSFAKVFES
                     GDIIFGGFNNAPGAINLQHADGSSTYGLGPWISPDDKFVGFVFETPAAAYYAWVRLQV
                     DECGYTIKDYAYSPVPIIAGAGIPADICESPAATYVTGIEAEKAKISWDAVPGATTYN
                     VRFRAVGEIDWHEKTVDAPKTFVKLKALSCDTDYEWQVQAVCDGGPSDYTDIAGFSTL
                     SCRIGDVADAGFDIYPNPTNTTLNVELENELTGDAFMQITDFTGKIVMQQNLSATLTG
                     IDLTELPSGIYILTVFENGNMNKKEFIKQ"
     gene            complement(4026..4250)
                     /locus_tag="PLB46_11195"
     CDS             complement(4026..4250)
                     /locus_tag="PLB46_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77130.1"
                     /translation="MYPNPANTLLNVELENELNGDVFMQITDFTGKIVLQQNLTSTLT
                     GVDLTELPSGIYILTVFENGNMNKKEFIKQ"
BASE COUNT         1432 a          781 c          642 g         1475 t
ORIGIN      
        1 tttcaaataa ttaaaaagtc tattatatca acgtactaaa aatggggagc ttacagtatc
       61 taatttaaca gcagtgtcta atcaggtttt acagacaagc gaagaattaa aaatccttac
      121 cgaaaaccta aacaacaata atacttctgt tgcaggtgtt ttattaaatg atgaatctgc
      181 agctgttaat ttaaaacaat cattaatcaa tttggaaagc agctctcaaa aattaaatga
      241 aaacatggaa gcattgcagc ataatttttt attccggaga tattttaaaa ataaagacta
      301 atatcatgta agatgaaccc acataacttt gtacacttcc atttcaacct gacttttgcc
      361 tattgttgta atcggcaata gtttttcaag ttaaaaaatt taaatcttaa aagtccagta
      421 tttctcccca aatacttttc tttttatact gttttggatg ttgctgatgt ttgtagtcat
      481 tgatttcgtt ttgtggatga gctgactttg atttgtttat aaaacgctca ccactgcgtt
      541 caattaattt atctaattca ccacgatcca gccatacacc acggcaagca ggacaataat
      601 ctatttctac cccttggcga tctgacatca ccaactcttt atcaatacat aaaggacatt
      661 tcataacgaa gttttcttta aaactatgta gacagaaata tgttcaatgt tttaaaaaaa
      721 attaacattt catctttttg aaataaaaaa acctccggac aatgaatata ggagattgat
      781 caaaactttt gcaaaaatta tagcttaata aattctgaac gttgaacctc atctccgttg
      841 gcaacttcaa gaacatctat ttctgcaggt atttcctgaa ggtctattat tgcactatag
      901 tcgtcaacaa tttgtgcgaa aacaacttta ccggcaaagt caataatcat taacgaacca
      961 ttaacagggg tatcttcgaa atcaatatgc tgggcaacaa cagcaggatt aggataaatg
     1021 acgaatctgc tgctttaaaa taatcattaa tcaatctgga aagaagttct caaaattaaa
     1081 tgaaaacatg gatgcactgc aacataattt tttgtttcga cgatacttta aaaataaaaa
     1141 tagtaaatag gtttattggt ttcttataaa cctatttact aaaaataaaa ttacaatatt
     1201 gctattgtga actattcctc ctcatctttt tcaattaatt tctgcactga aatacccatt
     1261 ttagcaatcg tattttctgt cgatacaagc gtaacttctg atggtttagg aacacgaagt
     1321 attgttctat tatcaacttt tacggcacaa gcactgttgt gtccataatt tacataggca
     1381 taaccgggtg tttttgctaa aatatgggct tctaattctt ctttagtaac aatttgcgat
     1441 ttaaaaggat acactttgct acccttgccc ataatatcat catcttttgc atcttcctca
     1501 ctaatgagta gcgttaaggt ggcaagttct ttactctttt cattaatttg gatcatcata
     1561 tctttatcac ttatatcagc catctttgat cttatgtacc aatcgatata agttaaccca
     1621 aaagcaatgt ctgttgattg cggcacgata tgtcccattg ggattgtcat ttgatttgga
     1681 aactgtaaat tttctaacaa agcaacacgc attacagtaa aatgggaaga cttgctccga
     1741 attttattcg ttttttgtat acctccaata gcagaattat caaatgtaat atcataatta
     1801 ccaataacgt atgttttgcc tgatgttcca gtggaagtct tttcttccat cctttccata
     1861 attataacaa catattgaga tttatcatca cctttaatat ctttaatatc ctcataagac
     1921 atgtatttta tggatttatt taatgtccat aatgagtcta ccattttttt gatatccgca
     1981 ttaaactcct taattgttgc tttatatgtt gccagttttt cagggtcctt atttttgtcc
     2041 aattttttta caatatcatc atcgtattct aaagtggtta caattaattt acgttcggca
     2101 aattcagtag cctttttaac gcttaattct gtatacccat ctatcggata agaatcgaat
     2161 tgtccatgtg cgttttgaat agagccaaat atcacaagac ataaggcaat ggtgagctgt
     2221 agttgtttca tgttttatat ttaattgaca acaaatttat atgaatttat taaaatcagt
     2281 ggactttatg taactaatcg tagttctgca agtaaaattt aaagttaaat tcacctattt
     2341 ttttatgtaa aaaaggttgt cccttaccaa gaaacaacct cttttataag ttaacattgc
     2401 tttattgttt aataaactct tttttgttca tattgccatt ttcgaaaaca gtcaaaatgt
     2461 aaattccgga tggcaattca gttaaatcaa taccggtaag ggttgctgat aaattttgtt
     2521 gcataacaat tttgccggta aaatcagtta tttgcataaa ggcatcaccg gttaattcgt
     2581 tttccaattc aacatttaac gtcgtatttg tagggtttgg gtatatgtcg aaaccggcat
     2641 ctgccacatc tccaattcta catgaaagtg ttgaaaaacc tgcaatatct gtataatctg
     2701 agggcccgcc atcgcaaacg gcttgcacct gccattcata atcagtatcg caactaaggg
     2761 ctttcaattt tacgaatgtt ttaggtgcat caacagtttt ttcatgccag tcgatttccc
     2821 ctactgctct gaaacgaaca ttataggttg ttgctcctgg aactgcatcc caactgattt
     2881 ttgctttttc tgcttcaata ccagttacat aagtggcagc cggagattcg catatatcag
     2941 ccggaatacc cgcacctgct ataataggta caggtgaata ggcataatct ttaatggtat
     3001 atccacattc gtccacttgt aagcgaaccc aagcataata agcagctgca ggagtttcaa
     3061 atacaaatcc gacaaattta tcatctggag aaatccatgg gcccaaacca taagttgaac
     3121 ttccatcagc gtgttgtaaa ttaattgctc cgggggcatt gttaaaccca ccaaaaataa
     3181 tgtcgccact ttcaaatact ttcgcaaacg aatacattgc taacgaatca tattgataac
     3241 taataaaaca atatgaataa tttggcacga cgaaacccaa gcaaccaact gaaccataaa
     3301 aattaccgat atggtcaaaa tgcacttcaa attctccatc tagatttaaa tccatgtaat
     3361 aatcaaccgg atcaccagaa atatacacat ctggatctat atcggtgtaa ataatttgtg
     3421 acttaagttc atttatactg gctaataaaa aagctgacgc agccccggaa taggatagta
     3481 atttagtttt catagaattt aatttttctt aaatttagag agaatttttt aaaattcaaa
     3541 aatctaataa gtattcttaa ttaccttaat aatcagcttt ttctcacaca tttatgactt
     3601 aacaatggtt aaaaggttat acaaattacc cgtaaaacca tttaaacggg gttactcctt
     3661 aaaaacgatc ttggaattag tacccacctc attattttta ttcaaatcgc acagactatg
     3721 cagtgttgta gcatttttaa tgtttaattc cgtataccca tcaattggat aagaatcgta
     3781 ttgtccatgt gcattttgaa cagcaccaaa tatcacataa cattaggcaa tggtgcgtgg
     3841 aagatgtttc atgttttata ttgaatagaa aacacatatg cactaatttc caaacatcag
     3901 ctgcctttat ggaatacaac taagtgctat cagtaaaatt tatagttaaa ttcatgcata
     3961 attaatatgc aaaaagaggt tattccctat caagaaacaa cctcttttat aagttaacat
     4021 tacatttatt gtttaataaa ctcttttttg ttcatattgc cattttcgaa aacagtcaaa
     4081 atgtaaatgc cagaaggcaa ttcagttaaa tcaacacctg taagtgttga tgttaagttt
     4141 tgttgcagaa caattttacc tgtgaaatcg gtaatttgca taaagacgtc accgtttaat
     4201 tcgttttcca attcaacatt taacaaagta tttgcagggt tcgggtatat gtcgaaacca
     4261 gcatcagcta aatcgccaat tctacatgaa agtgttgaat tggcgattta gctgatgctg
     4321 gtttcgacat
//
LOCUS       DAORKW010000298         4324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_972_length_4324_cov_3.741157, whole genome shotgun sequence.
ACCESSION   DAORKW010000298 DAORKW010000000
VERSION     DAORKW010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_972_length_4324_cov_3.741157"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="PLB46_11200"
     CDS             complement(<1..1608)
                     /locus_tag="PLB46_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77131.1"
                     /translation="MKQILLLLQVCVISLSVIAQTNTFPTIGNVGIGTTTPTQLFQVY
                     GGIAQFGGTSDYTRFATDGDLTFIGNADYLVGADRFAFRYQGNPNFGLVFSASNSRYE
                     FRDGTGVPVFWQGGNGNAYIKGFLAIGSTTTPSAALDITSTNAAAIKINPFNTGAGNT
                     GNLRFQELTANGNNYVGFKSPDNIPNNKIWVLPNADGTNGQYLKTDGAGNLGWATDAG
                     TTYFAGAGISFSGTTINNTAPDQIITLSGTGAASVAGTYPNFTINSVDNNTIYTAGSG
                     IDVTGTVITNLSPDQTITLAGDGATTITGTYPDFTISSTDFNTTYTAGSGIDITDATI
                     TNTAPDVIITLTGTGATDITGTYPVYTITSTDENTTYSAGAGITIADTVIVNTGDLNP
                     ADDANIALSNLSSTAINQSLIPDGNNTRDLGNTAASWKNIYTDETIYFDDNKFISNIG
                     GSVFTGVNAGNVNAGANNSGYGNHALNANTTGTQNVAVGENAMYSNTTGRANTAIGFY
                     TMYLNTNGINNVAIGQSALYSNTTINYT"
     gene            2071..2901
                     /locus_tag="PLB46_11205"
     CDS             2071..2901
                     /locus_tag="PLB46_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF77132.1"
                     /translation="MASSMYQPKVYVVHTHQLRRIFYSVSSDLHRIWLLRFAKGSIKI
                     DDKEFKIHNHMVVFAYPGQIIEVSSIGPFEGYCVAFNESFFYQHTEHKEMLFKAPFFG
                     YMMALPIAGITSKQFTMFGELLKQMVKEYKLQSMWYEQILLSMLNVFLLRIKRFKWAQ
                     NSQVHRSDYTTLHLVMMFKQLIRLNCHKEHSVSWYAKQLQQSPNYLNLMVKQVTGHPA
                     GNLIATQLIIEAKRQLIHSKLSAKEIAFALGFYDHSYFTKFFKKHTGNSPLEWFNKNK
                     "
     gene            3071..>4324
                     /locus_tag="PLB46_11210"
     CDS             3071..>4324
                     /locus_tag="PLB46_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77133.1"
                     /translation="MKSIITLLIFSLLTYYSAGQAPGINWQNAIGGNATDLATVIEPA
                     YGGGYFIGGYSNSDAAFDKSERYKGATALYDYWIVKCDAYGNILWENTIGGRREDWNS
                     CIYPTDDNGCILGGSSASSVGADKTENSIGIFGQTDYWILKLDANGEIVWQNTIGSTT
                     IDFMHDMCATPDGGYMLFGESYGGIDGDKTVGSYGGYDYWLIKVDATGTILWQKSYGG
                     SAYDYARKIIPAIGGGYYLAGISYSNINGTKFENSRGMGDYWVIKIDDIGNEIWQKTI
                     GSVAEDELTDILETQDGDLIICGTSYGAIGGDKTMVGFGNADYWILKLNNDGELIWQQ
                     AYGGSNVDGAAAIAKTSTGQIRIAGHSSSPISGNKTVASTVATDFWILQISPTGDIQW
                     QQNVAGNNQDVLSDMIVNASDETVFV"
BASE COUNT         1363 a          706 c          865 g         1390 t
ORIGIN      
        1 agtataatta attgtagtgt tggaatataa tgcactttgc ccgatggcta cattatttat
       61 accattggta tttaaataca tcgtataaaa accaattgca gtatttgccc tgccggttgt
      121 attagaatac atggcatttt cacctacagc tacattttgt gttcccgttg tattcgcatt
      181 taatgcatga ttaccataac ctgaattgtt tgctccagcg tttacatttc ctgcatttac
      241 tcctgtaaaa accgaaccac caatattgga aataaattta ttatcatcaa aataaatggt
      301 ttcatcggta taaatatttt tccatgatgc agcggtatta cctaagtcac gtgtgttatt
      361 tccatccgga attaatgatt gattaattgc agttgatgat aaattagata acgcaatatt
      421 ggcgtcatct gcaggattta aatctcctgt attaactata actgtgtcag ctatggtaat
      481 gcctgcgcct gcagaataag ttgtattttc atcggttgag gtaattgtat aaaccggata
      541 agtcccggta atatcagttg cgcctgttcc ggttaaagta attattacat cgggtgctgt
      601 atttgtaatt gtcgcatcgg tgatatcaat tccggaacct gcagtataag ttgtattgaa
      661 atcagttgat gaaatagtaa aatcaggata agtaccggta attgttgtag caccatcacc
      721 ggcgagagta attgtttgat caggagataa atttgttatt accgttccgg taacatctat
      781 accagaacca gcggtataaa tggtattatt atcaaccgaa ttaattgtaa aatttgggta
      841 ggtgcccgca accgatgctg ctccggtacc gcttaatgta attatttgat cgggtgcggt
      901 attatttatt gttgttcctg aaaaactaat tcctgctcct gcaaaatatg ttgttcctgc
      961 atctgttgcc cagcctaaat tacctgcccc atctgttttt aaatattgac cattggtgcc
     1021 atctgcgttt ggcaataccc aaattttatt atttggaata ttatccggcg atttaaaacc
     1081 gacataatta tttccattgg cagtaagttc ctgaaaacgc aaatttcctg tatttcctgc
     1141 accggtattg aacggattta ttttaattgc cgcagcattt gttgaggtaa tatccaatgc
     1201 ggcgcttggt gtggttgttg agcctatagc taaaaaacct ttgatatatg catttccgtt
     1261 tccgccctgc cagaaaacag gaacaccggt cccatcccga aattcgtatc ggctgttgga
     1321 agcactgaat actaatccga aatttgggtt tccctgataa cgaaaagcaa aacgatcggc
     1381 cccgacaaga taatcggcat taccgataaa tgttaaatcg ccatctgttg caaaacgcgt
     1441 ataatctgac gtaccgccga attgtgcaat tccaccataa acctgaaata attgagtggg
     1501 tgtggtggtg ccaataccaa cattaccaat tgtaggaaac gtatttgttt gagcaataac
     1561 tgataaactg attacacaaa cttgcaacaa gaggagtatt tgtttcatat acataatatt
     1621 atttgttaat tgtagtatta aagatatgag tttatcattt aaccaaagaa tctcgaacgg
     1681 taataattaa attgcctcag cgttcaatat ataaatagtt gatttacata ttttcttttg
     1741 aatagggcct attatggtat caaattatat tgccagacaa tttgcaactg taaacaacaa
     1801 tacctaattc gaatgcccgc tggtgtagta gtttcaccaa gtttaggtac aaccaattta
     1861 tatacaataa tacgttgaaa aacgcggcgt tttaggttgt ttttttcaga accttagttt
     1921 gattctccta attcaattag gatgtacaat tgtttggaat aataccaata aatcgtgaaa
     1981 aagtgcaata ataattttat acctttgtat tgaagagttt gataccataa ttaataagct
     2041 ttttttgaaa ataaattttt aaattaatca atggcatcat ctatgtatca accaaaagtg
     2101 tatgtagtcc acactcatca attgaggcgt atattttatt cagtttcttc agatttgcat
     2161 cgcatttggt tattgaggtt tgcaaaaggc tcaataaaga ttgacgataa agagttcaag
     2221 attcataacc acatggttgt ttttgcatat cctgggcaga ttattgaagt ttccagcatt
     2281 ggcccgtttg aaggatattg tgttgcattt aatgaatcat tcttttatca gcatacggag
     2341 cataaagaaa tgctatttaa agctccgttt ttcggttata tgatggcact accaattgca
     2401 ggtataacat caaagcaatt taccatgttt ggtgaattgt tgaagcaaat ggttaaagag
     2461 tataagctcc agagtatgtg gtatgaacag atacttctga gtatgttgaa cgttttcctg
     2521 ctccggataa aacgtttcaa atgggcacaa aatagccagg tacatagaag tgactatacg
     2581 acacttcatt tagtgatgat gtttaaacag ttgatcagat tgaattgtca taaagaacac
     2641 tcagtttcgt ggtatgccaa acagttgcaa caaagtccta actacctaaa tttaatggtg
     2701 aagcaagtga caggccaccc agctggcaac ctcattgcta cgcaacttat aattgaggcg
     2761 aaacgtcaat taatacattc aaaattgtca gctaaagaaa ttgcttttgc tttaggattt
     2821 tatgatcatt catactttac caagtttttt aagaaacaca ctggaaatag cccacttgaa
     2881 tggtttaata aaaataaatg acaaatacaa tttttcgttg caaaattact attatgaatt
     2941 tttaagatat tattaatttt ggataataat gtattttact tgtattagca tggcgggtaa
     3001 aataagttca gagggaataa agtaaaattc ttgccattta tattttgtta gcattaaaaa
     3061 attatttaat atgaaatcaa taatcacact attaattttc tccttactca cttattacag
     3121 tgcgggacaa gcaccaggta ttaactggca aaatgctatt ggaggtaacg caacagattt
     3181 ggcaactgtc attgaacccg catacggtgg ggggtatttt attggtggat actccaactc
     3241 agatgccgca tttgataaat ctgaaaggta taagggagca actgcactct atgattattg
     3301 gatagtaaaa tgtgatgcat acggtaatat tttatgggag aatactattg gtggtaggcg
     3361 agaagactgg aattcttgca tatatcctac cgatgataat gggtgcattt tgggtgggtc
     3421 atccgcttca tctgtaggcg ctgacaagac tgaaaattca ataggcattt ttgggcaaac
     3481 ggattactgg attttaaaac ttgatgcgaa tggagaaatt gtctggcaaa ataccattgg
     3541 tagtacaaca attgatttta tgcacgatat gtgtgctaca cctgatggcg gatatatgct
     3601 ttttggcgaa tcgtatggtg gtattgatgg ggataaaacg gttgggagtt atggaggata
     3661 tgattactgg ctaatcaaag tggatgctac tggaacaatt ctttggcaaa aaagttatgg
     3721 tggatcagcg tatgattatg caaggaaaat tattcccgca atcgggggag gctattacct
     3781 ggcgggtatt tcatattcaa atatcaacgg aactaaattt gaaaattcaa gaggtatggg
     3841 tgattattgg gttattaaaa tagatgatat aggcaatgaa atttggcaaa aaactatagg
     3901 gtcagtcgct gaagacgaac ttactgatat attagaaaca caagatggcg atttaattat
     3961 ttgcgggaca tcatatgggg caattggcgg tgataaaacg atggttggtt ttggaaatgc
     4021 tgattattgg atattaaaac ttaacaatga tggagaattg atttggcagc aggcctatgg
     4081 cggcagcaat gtagatggag ctgcggcaat cgcaaaaaca agtactggcc agatcagaat
     4141 tgcaggacac agttcttccc caatatctgg aaataaaaca gttgcatcaa cggtcgcgac
     4201 agatttttgg attttacaaa tttcgccaac tggagatata caatggcagc agaatgtggc
     4261 aggcaacaat caggatgttt tatctgacat gattgtaaat gccagtgatg aaacggtatt
     4321 tgtt
//
LOCUS       DAORKW010000299         4320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_977_length_4320_cov_4.607034, whole genome shotgun sequence.
ACCESSION   DAORKW010000299 DAORKW010000000
VERSION     DAORKW010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_977_length_4320_cov_4.607034"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..2750
                     /locus_tag="PLB46_11215"
     CDS             51..2750
                     /locus_tag="PLB46_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77134.1"
                     /translation="MKSQIFKAITFAFIFVTLTHTLKSQTILWQKVIGGSSTDKISAA
                     IIDNEGNILIAGTSGSDISEFKSEDNLGNVDFWIVKMDQEANIIWENTIGGNAEDFAY
                     CVQQTLDGGYIVGGYSFSDNSFDKTSDAFGEQDYWVVKLDYDGNVEWDKSFGGYDEDL
                     LFDIEVTSDGGYILMGESGSDDNGNKTLERCFSAISWPDYWFLKLDAEGEIVWQNMVG
                     GITNDWGREIVNTSDGNYIIAGRTDADIDCEKTADNLGAIDYYLTKIDPDGNYIWQKE
                     YGGDNNDFLEGIIPTSDNGFLLVGYSGSDISDSKTEGNIGNTGYMDYWVIKLDHYGEI
                     QWQNTIGGKSTDALLNCTQTIDGGYLLAGYSNSEIFADKTEAPYGNHDYWFVELNVFG
                     EVVDDFTIGGSSDDFLVEALQTNDYGYLLLGYSESNLTGIKTVAGLGSDDIWMVKIAH
                     DINIVEGTVAFDFNSNEIIDGDDFYCVNKLVQDETSGAITLTNASGKYVLGIESPGTY
                     ITSTPAIEYYSIVPTIYAGEFIDFGHIDTGIHFLIQPNGDFTDLCISAIRITPFRPGF
                     EAIYHLMYNNVGTTTAGGTVAMYPSEYIVYDSADVAPVLITADSILWNIADLSPFETG
                     SFNIYVSVIEAAPLDSTAISLFQLTPVVGDDGPECNYDTVVVVISGAFDPNNITVDKT
                     QLSVYEVPYQPKLEYTINFQNTGTDTAFLVQLINPLPEDLILASLIIKETSHTLNYFE
                     LDDDNNLVFQFADIQLPPTAIDEVNCHGFITYEMRTQTDLIEGDIIANEASIIFDFNT
                     PVITNTATTEIIVPTVGINNQPQLAISVKPNPFTNATTIYFNTYLNNAQIEVTDINGK
                     QIFKGFVSGTEWKFIPGGINSGLYFVHLTQEQIGTYSTKIVLL"
     gene            complement(3266..3982)
                     /locus_tag="PLB46_11220"
     CDS             complement(3266..3982)
                     /locus_tag="PLB46_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77135.1"
                     /translation="MKRIIIFVCFIFSTFVGFGQGFMYGGIGYNAAFLKSEGLDYVIN
                     RYNETRPYLDETMPNPHFYDGLTFHFGGAANAFFWDFGFTYRSCVVSATGVDASGTEQ
                     QRDVKNKWNTFDVGLGVNLGNSDTKALMIGINTGLNSEKSLTRADTPEDIGIANFAKV
                     NSQFKIGFEPFLQFIVATENGLGILFKPYYSWSPIKTDYTELNAYINQYTYINDPSPI
                     EGVLKGFGLSIMLVGYGEDD"
     gene            4090..>4320
                     /locus_tag="PLB46_11225"
     CDS             4090..>4320
                     /locus_tag="PLB46_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77136.1"
                     /translation="MRNFFILSVLALLTLVADGQIIVPVCGNLKGLPSQEECKEITER
                     KLILMIPALSEIKIKSLEKKKESGEIARIKKDY"
BASE COUNT         1419 a          690 c          811 g         1400 t
ORIGIN      
        1 agtttaacaa ttaatcaata tttttgacta ccgtaaatac acgttttgcc atgaaaagtc
       61 aaatatttaa agccatcaca tttgcattta tttttgtaac gcttacccat acactaaaaa
      121 gccagaccat tttatggcaa aaggtgattg gtggtagttc aactgacaaa atttctgctg
      181 caattattga taatgaaggg aatatactaa ttgcaggtac ctccggttcc gatataagtg
      241 aatttaaatc tgaggataat ttaggcaacg ttgatttttg gatcgtaaaa atggaccagg
      301 aagcgaatat catatgggaa aataccattg gcggcaatgc agaagatttt gcctactgtg
      361 ttcaacaaac tttagacggg ggatatatag ttggaggata ttctttttca gataactctt
      421 ttgataaaac ctcagatgca tttggtgaac aggattattg ggtggtaaaa ttggattatg
      481 atggcaatgt agaatgggat aaaagttttg gaggatatga cgaagatttg cttttcgaca
      541 ttgaagtaac tagtgatggt gggtacatcc tcatgggaga atctggttct gatgataatg
      601 gtaataaaac tttagaacga tgttttagcg ccatatcttg gccagactac tggtttttga
      661 aattagatgc agaaggtgaa attgtatggc aaaatatggt gggtggtatt actaacgact
      721 ggggtaggga aatagtaaat accagcgatg ggaattatat cattgcggga cgaaccgatg
      781 ccgatataga ttgcgaaaaa acagcagata atttaggtgc aattgattac taccttacaa
      841 aaatagaccc tgatggaaat tatatttggc aaaaggaata tggtggggac aataatgatt
      901 ttttagaagg gataattcca acatcagata atggattttt attagttggc tattcgggtt
      961 cagatatttc agactctaaa accgaaggta atattggcaa taccggttat atggattatt
     1021 gggtgataaa gttggatcac tatggtgaaa ttcaatggca aaatacaata ggagggaagt
     1081 ctaccgacgc tttattgaat tgcacacaaa caatagatgg cggctatttg ttagcaggtt
     1141 attccaattc ggaaattttt gcagataaaa cagaagcgcc gtatggtaat catgattatt
     1201 ggtttgttga attaaacgtt tttggtgaag ttgtagatga ttttactatt ggaggcagtt
     1261 ctgacgattt tttagttgaa gcacttcaaa ctaatgacta tggctattta ttattgggct
     1321 attctgaatc taatttaaca ggaattaaaa cagttgccgg tcttggtagt gacgatatct
     1381 ggatggtgaa aattgcccac gacatcaata tagtggaagg tactgttgct tttgatttca
     1441 atagcaatga aataattgat ggtgatgatt tttattgcgt gaataaattg gtgcaggacg
     1501 aaacctcagg agctataacc ttaaccaatg catcaggtaa atatgtctta ggtatagaat
     1561 cacctggaac atatattacc tcgactcctg ccatcgaata ctattctatt gtaccaacta
     1621 tttacgctgg tgaatttatt gattttggac atatcgacac aggaatacat tttcttattc
     1681 aacccaatgg tgattttacc gatttatgta tatctgctat tcgcataaca cctttccggc
     1741 caggttttga agctatttat cacttgatgt acaacaatgt aggcacaaca acagctggcg
     1801 gaacagtagc catgtatcct agtgagtaca ttgtatacga ctccgcagat gttgctccag
     1861 tgctcatcac ggctgattca atattatgga atattgcaga cttatcgcca tttgaaaccg
     1921 gttcatttaa tatatatgtt tcggtgattg aagcagcacc acttgattca actgctatat
     1981 ctttatttca actcacacct gttgttgggg acgatggtcc tgaatgtaat tacgatactg
     2041 tagtggtggt tattagtggc gcatttgatc ccaataatat tacagtagat aaaactcaat
     2101 tgtctgttta tgaagtgcca tatcaaccga aattggaata cacgataaat tttcaaaata
     2161 caggaactga tactgcattt ttagttcagc tcattaatcc attgccggaa gatttaatac
     2221 tagcaagttt aattattaaa gaaacatcgc ataccttaaa ttatttcgaa ttagatgatg
     2281 ataataattt agtttttcaa ttcgctgata ttcaactccc accaactgct atcgacgaag
     2341 taaattgtca tggctttatt acgtatgaaa tgcgaacaca aactgattta attgagggag
     2401 atatcattgc caatgaggca tctattattt ttgattttaa cacaccggtt attacgaaca
     2461 ccgccactac cgaaataatt gtaccaacag ttggaattaa taatcaacca caattggcca
     2521 tatctgtaaa gcctaatccg tttaccaatg ctactacaat ttatttcaat acgtatttaa
     2581 ataatgcaca aattgaagtt acagacatta atggcaaaca aatttttaaa ggttttgtga
     2641 gcggaactga atggaaattt attccgggtg gcattaattc gggcttgtat tttgtgcatt
     2701 taacacagga acaaattggc acatacagca caaaaattgt tttactttaa gcggttactt
     2761 taaataccat ttattttagg tgcacctaag atgaattgct ttgtttttaa aggtttcgct
     2821 tgtgtaaaat ttaatcttat caattgattg tcaatgtgtt aaccttaaaa aacacagcga
     2881 attttgcttg tcacaaaagc gcattgtttg tatcaattta ccaattaaaa ccattgtttg
     2941 aaatggtaaa tttgttcaat cacattttaa acccttttac aacttatgaa aaaagcactt
     3001 ttatttattg cctccggaat catagtataa gaaaatgcag gtcttaccaa tcaggtcgac
     3061 gaatatgaca tgaaaatagt ttattatttt ataggtgata atacgtttga attaagtgat
     3121 tgatcaaccg gctcaggtgg cacatgggga tatttttggg cgtttaaagg agaattccat
     3181 tctacatttt aatgtttgat ataaaaagta aaaccggcaa agtgtccata tacattgccg
     3241 gttatttatt tgctaatttt tttatttagt cgtcctcgcc atatccaact aacataatgg
     3301 ataaaccaaa tccttttaaa acaccttcaa ttggtgatgg gtcattaata taagtatatt
     3361 ggttgatgta tgcatttaat tcggtataat cggttttaat cggcgaccaa ctgtaatacg
     3421 gtttaaataa aatgccgaga ccgttttctg ttgctacaat aaattgtaaa aatggttcga
     3481 atcctatttt aaactgggaa tttacttttg caaaattggc aatccctata tcttccggag
     3541 tatcagcacg agttaatgat ttctcactat ttaaaccggt atttatccca atcattagtg
     3601 ctttggtatc actattacca agatttacac caagacctac atcaaaggtg ttccatttat
     3661 tttttacatc tcgttgttgc tcagttcctg aagcatcaac tcccgtagca cttacaacac
     3721 aacttctgta agtaaatcca aaatcccaaa aaaatgcatt cgcagcccca ccaaagtgaa
     3781 aagtaagccc gtcataaaaa tgcggattag gcatagtttc atccaaatag ggacgggttt
     3841 cattatagcg gttaatcaca taatctaaac cttccgattt taaaaaggcg gcgttataac
     3901 cgataccacc atacataaaa ccttgtccga aaccgacgaa agtggaaaat ataaagcaca
     3961 caaatattat tatgcgtttc attttggtta attttaatgt aaagttaggt atagtatttg
     4021 tgatttttaa agtcaaaaag cacgcctcgc cttttttacg gaaaagagct aattttgtaa
     4081 aaaaacatta tgcgtaattt ttttatatta tccgttttag cattattaac actggtagct
     4141 gatggtcaga ttattgtccc tgtttgtggg aacttaaaag ggttaccctc ccaagaggaa
     4201 tgtaaagaaa taactgaaag aaagttaatc ttaatgattc cggcacttag tgaaatcaaa
     4261 attaaatcgc tggaaaagaa aaaagaaagt ggagaaatag cacgtataaa aaaggactat
//
LOCUS       DAORKW010000300         4319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_978_length_4319_cov_3.673546, whole genome shotgun sequence.
ACCESSION   DAORKW010000300 DAORKW010000000
VERSION     DAORKW010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_978_length_4319_cov_3.673546"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLB46_11230"
     CDS             complement(<1..563)
                     /locus_tag="PLB46_11230"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRF77137.1"
                     /translation="MNQFGITIQAYVQAVGDIRVHKHYSELALDTIENNIVRCPDQEI
                     ATQMIDRIAAIKKQGDTIGGVITCVIKNTPAGIGEPVFDKLHAELGKAMLSINAAKGF
                     EYGSGFEGTSMLGSEHNDIYETNAGGKIVTRTNHSGGIQGGISNGMDIYFNVAFKPVA
                     TILRPQESVNNAGDAVIVEGKGRHDPCV"
     gene            complement(514..996)
                     /locus_tag="PLB46_11235"
     CDS             complement(514..996)
                     /locus_tag="PLB46_11235"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRF77138.1"
                     /translation="MAGNVFGNLFRISTFGESHGVALGVVIDGCPPGIVLDLDFMQAE
                     MARRKPGQSKIVTQRQEGDAFEILSGVYEGRTTGTPIGIIIRNEDQKSKDYNHLLDAY
                     RPSHADFTYDQKYGFRDHRGGGRSSGPGNCCQSYCRKYCKTVFESIRNYHSGVCAGCW
                     "
     gene            1065..2285
                     /gene="hutI"
                     /locus_tag="PLB46_11240"
     CDS             1065..2285
                     /gene="hutI"
                     /locus_tag="PLB46_11240"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRF77139.1"
                     /translation="MAAILIRNIKTLWQTDEGERTVVKGKAMATIPFIDNAWLWIEAG
                     KIMDFGPMETMPELNIHTIDATGKMVLPAYVDSHTHIVYAANRDEEFLMRLKGKSYEE
                     IAAAGGGILNSATKLGAMDAEELYDNAFERLENLIALGTGTIEIKSGYGLNTAAEIKM
                     LRVIRKLKENSRATIKSTFLGAHALPAEFKNNRKGYIDLIINDMLPKIADEGLADYCD
                     VFCEQGYFTNEETETILTAAWKYHIKPKIHVNQFTNTGGIQTGIKNNAISVDHLEYLG
                     DAEIDALLQSNTLPVALPGCSFFIQIPYTPGRKIIDAGLPLVLASDYNPGSCPSGNMN
                     FVIALACIQMKLTPEEAFNAATLNGAAALELSKIVGTIAKGKSAKIIITKPIAALTEI
                     PYRFGENLIDQVLI"
     gene            2499..2693
                     /locus_tag="PLB46_11245"
     CDS             2499..2693
                     /locus_tag="PLB46_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77140.1"
                     /translation="MVQTLVGRNIICCIDSADDVIRDDAGDNIPGVALMDKMFTIAFL
                     STNFWGSRGEGRGGVEVCEN"
     gene            2839..4017
                     /locus_tag="PLB46_11250"
     CDS             2839..4017
                     /locus_tag="PLB46_11250"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRF77141.1"
                     /translation="MKEVYIVSVARTPIGSFGGGLASLTAPQLGAHVIKAAVERAGIQ
                     GTDVQEVYMGNVLPANIGQAPVTQAMLAAGLPDTVNSTTINKVCASGMKAIMIGAQQI
                     MLGMSDIVVAGGMESMSNTPYYLTKERWGAKMGNSEVVDGIIKDGLWDPYGNKHMGSC
                     GETCAREYNFSREDQDNYAIESYKRAAAAWKNGSFKNEVVPVVIPQKKGDPIVVSEDE
                     DYTKVKFDKVPTLAPAFEKTGTITAANASNINDGASAVVLMSKERAEAMGIKPLAKIL
                     AFADAQQTPEWFTTTPGKAMPKAIAAAGLSISDIDYFEINEAFAVVALANMKDMGISH
                     DKTNIYGGGVSIGHPIGSSGARITITLLSALQNNNARYGCAGICNGGGGASAIVIERM
                     "
BASE COUNT         1313 a          832 c          912 g         1262 t
ORIGIN      
        1 acacaagggt cgtgtcggcc ttttccttcc acgataactg catctcctgc attatttaca
       61 ctttcctgtg ggcgcaaaat agtcgccacg ggtttaaatg caacattaaa ataaatatcc
      121 atgccattgc tgataccacc ttgaatacct ccgctgtgat tggttctggt tacaattttt
      181 cctcctgcat ttgtttcata aatatcgttg tgttctgaac ctagcatgga agttccttca
      241 aagccgctgc catattcaaa tcctttcgca gcatttatac ttaacatagc tttgcctaat
      301 tcagcatgta atttatcgaa taccggttca ccaatgccgg caggtgtatt tttaattacg
      361 catgtaatca caccaccaat ggtatcacct tgttttttta ttgctgcaat acggtcaatc
      421 atttgcgttg caatttcctg atcgggacaa cgcacaatat tattttcaat ggtatcgaga
      481 gctaattcgg aataatgttt atgcacccga atatcaccaa cagcctgcac atacgcctga
      541 atggtaattc cgaattgatt caaatactgt tttgcaatac ttcctgcaat aactctggca
      601 gcagtttccc ggacccgaac tccgcccgcc tcccctatgg tcgcgaaaac catatttttg
      661 gtcgtaggta aaatcggcat gacttggtct gtaggcatcc aacaggtgat tataatcttt
      721 cgatttctgg tcttcattgc gaataattat accaattggt gttccggttg tgcgaccttc
      781 gtaaactccg cttaaaatct caaatgcatc accttcctgg cgttgtgtta ctattttcga
      841 ctgtcccggt ttacgacgag ccatttctgc ttgcataaaa tccaggtcga gtactatgcc
      901 cggaggacag ccatctataa ccacaccaag ggctacacca tgcgattcgc cgaaggtgct
      961 gatgcgaaac aagttgccaa atacattacc tgccataggt gcgaaattac gcagaagcag
     1021 gtattttttt ctgattacag cgatttcatg gatatttgca ggtaatggca gcgattttaa
     1081 tccgcaatat caaaacactt tggcaaaccg acgagggtga acgcactgtg gtgaagggca
     1141 aggctatggc aacaattccg tttatcgata atgcctggtt atggatagaa gcaggtaaaa
     1201 ttatggactt cggccctatg gaaacaatgc ctgaactgaa tatccacact attgatgcaa
     1261 cgggaaaaat ggtgcttccg gcatatgtcg acagtcatac gcatattgta tatgcagcta
     1321 acagagatga agaatttttg atgcgcctga aaggaaaatc gtatgaggaa attgcagcag
     1381 caggtggtgg tattttaaat tctgcaacaa aattaggcgc catggatgca gaggaattgt
     1441 atgataatgc atttgaacga ttagaaaatt taattgcttt aggcacagga actatcgaaa
     1501 ttaaaagtgg ttacggatta aataccgcag ctgaaataaa aatgttgcgg gtaattcgga
     1561 aattaaaaga aaatagtcgc gccaccatta aatccacttt tttaggcgca catgcattac
     1621 ctgctgaatt taaaaataat cggaaaggat atattgattt aattataaac gatatgttgc
     1681 ctaaaattgc agatgaaggt ttagcagatt attgtgatgt attttgcgaa cagggatatt
     1741 ttaccaacga agaaactgaa accattttaa cggcagcatg gaaatatcac attaaaccta
     1801 aaattcatgt caaccagttt accaataccg gaggcataca aaccggaata aaaaataatg
     1861 cgatttctgt tgatcattta gaatatttag gagatgcaga aattgatgca ttattacaaa
     1921 gtaatacttt accagttgca ttacccggat gttcattttt tattcaaata ccttacacac
     1981 ccggacgcaa aataattgat gctggtttac cacttgtttt ggcgagtgat tacaacccgg
     2041 gttcatgtcc cagtggcaac atgaattttg tcattgcact agcttgtatc caaatgaaat
     2101 taacccctga ggaggcattt aatgctgcta ctttaaatgg tgcggctgcg ttagaattaa
     2161 gtaaaattgt tggcactatt gcaaaaggaa aatccgcaaa aattatcatc acaaaaccaa
     2221 ttgctgcgct cacagaaata ccatatcgat ttggtgaaaa tctcattgac caagtcctga
     2281 tttagttgtt tactacaata tttaatttgt tgaaacggtc tactcctaaa ggctggtaaa
     2341 ttgttgctgt tcagaattgt cattttacag tctttttaca cttgttaaca aaattttaag
     2401 caacaaaata tacgcacagc cgtttatagt tgaaaatcgg gaattatgcc aaaccgaaaa
     2461 aatcaaagtc ctacaaaaaa ggacgaacaa ttcgttatat ggttcaaact cttgtgggga
     2521 ggaatattat ctgttgcatt gattctgctg atgatgtaat cagggatgat gcgggagata
     2581 atattccggg agttgccttg atggataaaa tgtttactat tgctttttta tctactaatt
     2641 tttggggttc gcgcggagaa gggagaggcg gagttgaggt gtgtgaaaat taatttttat
     2701 ttacttccat ctaattaaat taagtgcggg tataacctga aaacgaaatg gttatacact
     2761 tctgaactaa ttgagaaact gcactgttct caaccatatt attatctttg cggcgaaatt
     2821 taattaacct tactaattat gaaagaagtt tatatcgtat cagtagcccg cacaccgatt
     2881 ggaagttttg gtggtggatt agcctcttta actgctccac aattgggtgc acatgtaatt
     2941 aaagctgccg ttgaacgcgc aggcattcag ggcactgatg tgcaggaagt ttatatgggt
     3001 aacgtattgc cggctaatat tggacaggct cctgttacgc aggcaatgtt ggctgcaggt
     3061 ttgccggata ccgtgaattc tacaactatc aataaggttt gtgcttcggg tatgaaagcc
     3121 attatgattg gtgcgcagca aattatgctt ggcatgagcg atatcgttgt tgccggtggt
     3181 atggaaagta tgagcaatac cccttattac ctcaccaaag agcgttgggg agctaaaatg
     3241 ggcaacagtg aagtggtaga cggtattata aaagatggtc tctgggaccc ttatggcaac
     3301 aaacacatgg gttcatgtgg cgaaacctgt gcccgcgaat ataattttag tcgcgaagac
     3361 caggataact acgctattga aagttacaaa cgcgcagctg ctgcctggaa aaacggctca
     3421 tttaaaaatg aagtggttcc ggtggttata cctcagaaaa aaggcgaccc gattgtagta
     3481 agcgaagatg aagattatac caaagtaaaa ttcgataagg tgccaacttt agcacctgca
     3541 tttgaaaaaa caggtaccat tacagctgcc aatgcatcaa atatcaatga tggtgcttct
     3601 gcggtagtgt tgatgagtaa agaacgtgct gaagcaatgg gcatcaaacc tttagctaaa
     3661 attttagcat ttgccgatgc acaacaaacc cctgaatggt ttactaccac acccggtaaa
     3721 gcgatgccaa aagccattgc agcagcgggt ttgagcatta gtgatataga ttatttcgaa
     3781 attaacgagg catttgcagt agttgcctta gctaatatga aagatatggg tatcagtcac
     3841 gacaaaacca atatttatgg tggtggcgtt tctatcggtc acccaattgg aagctccgga
     3901 gcacgtatta caattacatt gttgagcgcc ctccaaaaca ataatgcccg ttacggttgt
     3961 gcaggtattt gtaatggcgg tggcggtgcg agtgctattg ttattgaaag aatgtaaatt
     4021 tcactgccgc tgttatacaa atctggatta ttcacagccg gtagcacaat aaaaagcata
     4081 aaaaaaccat ttatatgcct gagttttacg ctcaggcatt tttgttgtta gaccaaaaat
     4141 aatatgcaga ccatcaggac tatagttatc ggtttactcc tttttatcac caccggttta
     4201 cgggctcaaa cagccgaaat taaaggcatt acccccgaaa acctgatgaa actgcaacaa
     4261 tatgaggaca ccttaaagtt tttgggtgat tcgatggtgc agagtttgag ttgggaaat
//
LOCUS       DAORKW010000301         4309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_983_length_4309_cov_3.916549, whole genome shotgun sequence.
ACCESSION   DAORKW010000301 DAORKW010000000
VERSION     DAORKW010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_983_length_4309_cov_3.916549"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLB46_11255"
     CDS             <1..276
                     /locus_tag="PLB46_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77142.1"
                     /translation="RTNLDRLLRSYQAVKGENEILKQQVDAQRRGLADKNRKIEELEK
                     QFEIVKTAQSIAISSNSSELPEDKAEVKKRINELIKEVDNCIAMLND"
     gene            365..679
                     /locus_tag="PLB46_11260"
     CDS             365..679
                     /locus_tag="PLB46_11260"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRF77143.1"
                     /translation="MDTEESKLLNIHVTICDRPYRLKVKPEEEEIVRKAARIIAEKIK
                     DLQMQHAGNDKQDYLAMTVLTMMVEQLGAEQKQPEPIIAETDFSAELEQLNQQISKAL
                     HP"
     gene            748..2343
                     /gene="rny"
                     /locus_tag="PLB46_11265"
     CDS             748..2343
                     /gene="rny"
                     /locus_tag="PLB46_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRF77144.1"
                     /translation="MNDTLMYVIIGAVALIAGIIVGRLIFGQSKNQKQFAEREAEETI
                     SAAKKEAELIIQKSKNDADAYKKEKSLEIKDQMIQQKAEVEKERQQKVLKLEEQEMRM
                     KQKESQLGQQQQQYSKKEAELNTLKDNLAKQLEVVNLKKGELDKAYETHIRKLEDVAK
                     LTADQARTELMEALKDEARNKAMSHIKEIVEEAKLKANKEAKKIILQTIQRTAAEQAI
                     ENSVSVFNLDSDDQKGQIIGREGRNIRALEAATGVEFIVDDTPETIIISGFDPVRREV
                     ARLSLQRLVADGRIHPARIEEVVAKTKKQMEEHINEIGERTVIELGVTGVHPELIRMV
                     GRMRFRSSYGQNLLQHSREVANLRAVMAAELGLNPKLAKRAGLLHDIGKVPEEESELS
                     HALLGAKLCEKYGEHAAIVNAVGAHHDEIEMQYVISPIVQACDAISGARPGARREILE
                     SYLKRIKEMENLALANDGVEKAYAIQAGRELRVIVEAEKVSDKVAEELSFSIAQKIQD
                     EMQYPGHIKVTVIREKRAISFAK"
     gene            complement(2827..3726)
                     /locus_tag="PLB46_11270"
     CDS             complement(2827..3726)
                     /locus_tag="PLB46_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77145.1"
                     /translation="MKRLTVVILILIVKITISPLIACDQCGCSVAGSYNNVTAYAHNN
                     YMMFKAGYYKFSNGTATSKLHSEMFSVDAIVGYNITNRLHVLAFVPYRLNQFSDEDNT
                     YTVNGFGDMGLLGNYVLATNRDNMMAKYTYSLTVKAGVELPTGKFIDDYRALGVPANI
                     SAGSKTFDGISGLRYIYKRKSTTLIGDYTFKYNPENESNYRFGNQHTTTILAAQRFMQ
                     SKTVITPYVGVTGELDGTDLYHEIEQHGTKGETLLLNTGIELGFGDYIAGITADFPLY
                     ANFGEEAKSSPRFSLRFAYMLNN"
     gene            complement(3729..4133)
                     /locus_tag="PLB46_11275"
     CDS             complement(3729..4133)
                     /locus_tag="PLB46_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77146.1"
                     /translation="MKNSILAIAAIFAITFTACKKDENPIDEPIVYDVTFTIVEPEEG
                     AVFTSGDELHMEVDFEGTKALGNVEMLAINHTSGDTIAHFTATTAETFYMMHEHTDLS
                     VTEISECHFSVSAWQTNYADRISEEIDFTINP"
BASE COUNT         1475 a          849 c          844 g         1141 t
ORIGIN      
        1 cggaccaatt tagaccggtt gttgagaagt taccaggctg taaagggaga aaatgaaatc
       61 ctgaaacagc aggtagacgc tcagcgaagg ggtttagccg ataaaaaccg gaagatagaa
      121 gagcttgaaa agcagttcga aatcgtcaaa accgcccaat ccatcgcaat ttccagcaac
      181 agtagcgaat tacccgagga caaagcagag gtgaaaaaac gaatcaacga actgattaaa
      241 gaagtagaca actgcatcgc tatgctcaac gactgagcca aaacttacct taccccaatt
      301 agcattttcg taatctttgg ttaactttag tcccaattgg acaaactgaa taccataaaa
      361 accaatggat actgaagaaa gcaaattgtt aaatattcat gttacgattt gcgacaggcc
      421 atacaggctt aaagtaaagc ctgaggaaga agaaattgta agaaaagcag caagaattat
      481 cgctgaaaaa ataaaggacc tccagatgca gcatgccggt aacgacaagc aggattatct
      541 ggcaatgacg gtgctcacca tgatggtaga gcaattgggg gccgagcaaa aacaaccgga
      601 accgattata gcggaaaccg atttttcggc cgagttagag cagctcaatc agcagataag
      661 caaagctttg catccttaat cttctcccaa atcgccggtt cagcttggtt tttagtcagt
      721 aaggtccttc atcataaaac catatatatg aacgacactt tgatgtacgt tatcatcggc
      781 gcagtagcct tgatagcggg aattattgta gggcgactga ttttcggcca atcgaaaaat
      841 cagaaacaat tcgctgaaag agaggctgaa gaaacgattt cagctgcaaa aaaagaggcc
      901 gaactcatta ttcaaaaaag taaaaacgac gctgacgctt acaaaaaaga aaaatcactg
      961 gaaattaaag accagatgat tcagcaaaaa gccgaggtag aaaaagaacg ccagcaaaaa
     1021 gtgctgaagt tggaagagca ggaaatgcgc atgaaacaaa aagagtcgca acttggtcaa
     1081 caacaacaac aatacagtaa aaaagaagcc gaactcaata ccctgaaaga taatttagcg
     1141 aaacagcttg aggtggtaaa tttgaaaaaa ggcgaattag acaaagctta cgaaacacat
     1201 attcgcaaac tggaagatgt tgccaaatta acagccgatc aggcgcgcac cgagttaatg
     1261 gaagcattaa aagacgaagc ccgcaacaag gcgatgtcgc acattaaaga aattgtggaa
     1321 gaggctaaac tcaaagccaa taaagaagct aaaaaaatta ttcttcaaac tattcaacgc
     1381 accgcagccg agcaagcaat cgaaaattca gtatcggtat ttaacctcga cagcgacgac
     1441 caaaaaggtc agattattgg tcgtgaagga cgaaatattc gcgcattaga agctgcaaca
     1501 ggagttgaat ttattgttga tgatacacct gaaacaatta ttataagtgg ttttgacccg
     1561 gttcgccgtg aggttgcccg tttgagtttg caacgtcttg tagccgatgg tcgtattcac
     1621 cctgctcgta tcgaagaagt ggtggctaaa accaaaaaac aaatggaaga gcacatcaac
     1681 gaaattggcg aacgcacagt tatcgaatta ggcgttaccg gtgtgcatcc tgaattaatc
     1741 agaatggttg gccgcatgcg cttccgttct tcttacggtc aaaatttatt acaacatagc
     1801 cgcgaggttg ctaaccttcg cgcagtaatg gcagcagaat taggcttaaa tccaaaactc
     1861 gccaaacggg caggtttact ccacgatatt ggtaaagtac ctgaggagga atcagaattg
     1921 agccacgcat tgttaggtgc caaattatgc gaaaaatacg gcgaacatgc agctatcgta
     1981 aatgcagttg gtgctcacca cgatgagatt gaaatgcaat atgtaatttc tcctatcgtg
     2041 caggcttgtg atgctattag cggtgcccgt ccgggtgctc gtagagaaat tctcgagagt
     2101 tatttaaaac gtattaaaga aatggaaaat ctcgcgttag caaacgatgg tgttgaaaaa
     2161 gcatatgcca ttcaagccgg tcgcgaatta cgtgttattg ttgaagctga aaaagtgagc
     2221 gataaagttg ctgaagaatt atcattcagt atcgcgcaaa aaattcagga cgaaatgcaa
     2281 tatccgggtc atatcaaagt gacagttatt cgcgaaaaac gtgcaatttc ttttgcaaaa
     2341 taaattaaac agtaaatcat aaaaaggtct ccacacggag gccttttttt atggtattat
     2401 tttttggagc atcaacatcc tccttatcaa aacaatccga accggctgtc cgctgcaact
     2461 aacacaacta cgcattaaat ttaccgcatc aaacatcctt cactttctca ttcgccttaa
     2521 aaattaactt caaccacctc tccggctgtt tcccattggt acatcggtac atcaacacat
     2581 taacacatta gttagtttcc gctcccatcc gggctaacct tacatacttc accatcttta
     2641 caatttaatt agtactaaat aatcatttta ataaatgcag gtcattaatt tttaaaaata
     2701 tcatttatag aaaatagtat tattgtttta atgatgttgc catcaacaaa ttaaaagata
     2761 ccatccatcc taaaaacaaa aaaggccccc caacaacgaa ggaccttctt tattaatgga
     2821 tacgtgtcag ttatttaaca tataagcaaa acgcaaagag aatcgtggag atgatttcgc
     2881 ttcttcacca aagttggcat ataaaggaaa atctgctgta attccggcaa tgtaatcacc
     2941 gaaacccaat tcaattccag tgtttaataa taacgtttca cctttagtac catgttgttc
     3001 gatttcgtga tacaaatccg taccgtctaa ttcgccggta acaccaacat aaggagttat
     3061 tactgttttt gattgcataa atcgttgagc agccaaaatt gttgtagtat gttgattccc
     3121 aaaacgatag tttgattcgt tttccggatt atatttaaat gtgtaatcac caattaaagt
     3181 ggtacttttt cttttataaa tatatcgcaa gcctgaaatg ccgtcaaagg ttttagaccc
     3241 tgccgatata ttggccggaa cacctaaagc gcgataatca tctataaatt ttccggttgg
     3301 taattccacc ccggctttta ctgttaaact ataagtgtat ttagccatca tgttgtcgcg
     3361 attagttgcc aaaacataat tccccagtaa tcccatatca ccaaaaccat ttactgtata
     3421 ggtattatcc tcatctgaaa attgattcaa tcggtaaggg acaaacgcca acacatgtaa
     3481 acgattggtt atgttatatc cgacaatagc atcaaccgaa aacatttcgc tatgaagttt
     3541 gctggttgca gttccgttac taaacttata ataacctgcc ttaaacatca tataattatt
     3601 atgcgcatag gcagtcacat tattatacga cccggcaact gagcatccgc attggtcgca
     3661 ggcgataagc ggacttattg taattttgac aataagtatc aaaattacaa ctgttagtcg
     3721 tttcataatt atggattaat agtgaaatca atctcttcgc taatgcggtc agcataattt
     3781 gtttgccatg ctgaaacact gaaatgacat tctgatattt cggtaacaga taaatcagta
     3841 tgttcatgca tcatataaaa tgtttctgca gtagttgctg taaaatgtgc aatggtgtca
     3901 cccgaagtat gattaattgc aagcatctct acatttccca gagccttggt tccttcgaaa
     3961 tcaacttcca tatgtaattc atcgccagaa gtaaatacgg caccttcttc aggttcgaca
     4021 atagtgaatg ttacatcata aacaattggt tcatcaattg gattttcatc ctttttacat
     4081 gcagtaaaag taatagcaaa aatggcggca atagccaaaa tagaattttt catttggttg
     4141 aaattttaac gtaaataaat taataaagcg ggataaacat gttgtattac gctaaaattt
     4201 cgggaggcga gtaaacggca ggaacaccgt tacaggaggc tgtttttaaa ggcagggatt
     4261 gtttcagcag caagtttgaa gggacaaaag ttatttgatt atcactcac
//
LOCUS       DAORKW010000302         4289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_991_length_4289_cov_6.184695, whole genome shotgun sequence.
ACCESSION   DAORKW010000302 DAORKW010000000
VERSION     DAORKW010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_991_length_4289_cov_6.184695"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1292
                     /locus_tag="PLB46_11280"
     CDS             72..1292
                     /locus_tag="PLB46_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRF77147.1"
                     /translation="MKLKFEAVPKTINGAIQLEGSKSISNRLLILQALCDGGFEINNL
                     SPSDDTTTLKRLLALDETTCDVGAAGTTMRFLTAYFATRNCEKILTGSTRMQQRPIKI
                     LVDALAQLGASIEYLQNDGYPPIAIHGKKLAGGNVSIRADVSSQYISALLMIGPSLEK
                     GLHLTLEGKISSFPYINMTLKTMEELGVMSKIDGNSITVHPAKYQSKIMQAESDWSAA
                     SYYFSVCALSPNSTLKISGLFENSLQGDSVLPEIYRHLGVEAHFENTVLVLQQKGKCV
                     ETLEFDFSNCPDLAQTVAVTCAGLGVKAIFTGLESLRIKETDRTAALQTELKKFDVEF
                     WEEGEAWKLNGKTNAASVITINTYEDHRMAMCFAPLSLLHPQIVIEEPWVVKKSYPSF
                     WDDFSKLGFVSSIG"
     gene            complement(1235..2845)
                     /locus_tag="PLB46_11285"
     CDS             complement(1235..2845)
                     /locus_tag="PLB46_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HRF77148.1"
                     /translation="MKNSTFRLIIAGILLVVCNLSAHVVQAQTNMPVKTKKDSSEIQI
                     RSADYLEIIQKKNVSVNRLINNVILEQNDLILYCDSALLFKSENMAKIYGHVHLIQAD
                     SIHAYGDSAIYNGNSKIAKLYSNAKITDKSMTLTTDNLSYDLTTKIATYINGGQVTDG
                     EAVLTSQLGYYYANTNDAYFKNDVVLTHPEYGLKADTLQYNTATEKAIFRGPTTIFNK
                     ESTVYCEKGYYETNSGIAVFHQHAKLDNPPQSLTADSIYYNRETGIGKAYYNIVFTDT
                     SQQVLQYSNFAEYDEINGTIFSTGGSIAGFVMDTDTLFIGGDTIRSIQDSLKRKTLLV
                     FHNVKIYKSDLQGKCDSLSYADMDSVIRMYGAPIMWSDSTQFTADTINMIMRNKTLQE
                     IQLFTDGFIINEDDSLIYNQIKGRNIFGYFSNEDLVKIKAIGNGESIYYGKDDKDRYI
                     GVNKMFCSEIIILLSDKKFHRITFKNAPDSEFQPMQDAVLSDFLLKNFKWSYSERPMS
                     KDDLLTPDVPQPIEETNPSLEKSSQKEG"
     gene            complement(2862..3803)
                     /locus_tag="PLB46_11290"
     CDS             complement(2862..3803)
                     /locus_tag="PLB46_11290"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRF77149.1"
                     /translation="MKISNESKVGVLTAIGIALLFIGFNYLKGINIFHKGTDYFVEYG
                     SAGGLLVGDPIIIDGYSVGRVKSVALQEDQSGVNVVLNFSEDVNVPIDSRARIRGNLM
                     GEKYVEIYLGNATEMAKPNSNLLGEIESDLANTISAEFKPISDKVKSMLSQMDTAITV
                     LRSIFTEDVQNDLQKSMQSIKLTLESFNQSADRVNNIIIKEEGAIDSIISDVSDITNY
                     VNESENDIKSILSNLKAVTDSLNTIEWQELSTQFALAAENINTISQKINEGDGSLGKL
                     VNDEELYNELLASLKALDSAVAKFSEDPTIKLKLFGK"
     gene            complement(3858..>4289)
                     /locus_tag="PLB46_11295"
     CDS             complement(3858..>4289)
                     /locus_tag="PLB46_11295"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRF77150.1"
                     /translation="QSAIAESIFYAFRDYKNDMENNSGKVSAEKAQELEAQMDKKETP
                     VENPIAVAGDTKGLSFSVQFYASAVEEKNSKFSALSKENITFVKENGMYKYRLGPYST
                     LDEATKKQTFARENGYKDAFVIAINDGKRISIEEAKAIAKK"
BASE COUNT         1295 a          773 c          751 g         1470 t
ORIGIN      
        1 aattgtgatt acgatattca ggtaagcgat gccctaattg ccgatgcatt aacttattat
       61 tgcaccttaa catgaaatta aaatttgaag cggttccgaa aacaatcaat ggagcaatac
      121 aactcgaagg ttctaaaagt attagcaaca gattgctgat tttgcaggca ttgtgtgatg
      181 gtggttttga gattaataat ttatctccat ctgatgatac tactacatta aaacgtttac
      241 tcgcgcttga tgaaactaca tgtgatgttg gtgctgcagg aacaactatg cgttttttaa
      301 cggcttattt tgcaacgcgc aattgcgaaa aaatacttac cggctcaaca agaatgcagc
      361 agcgacctat taaaatttta gtagatgcat tagcgcaatt aggcgcttcc atcgaatatt
      421 tacaaaatga tggttatccg ccaattgcaa ttcatggaaa aaaattagca gggggaaatg
      481 tctcaatacg agcagatgtg agttcgcaat acatttctgc attactcatg attggacctt
      541 ctttggaaaa aggattacac ttaacattgg aaggaaaaat ttcttctttt ccctatatca
      601 atatgacctt aaaaacgatg gaagagttag gtgtgatgtc gaaaattgat gggaattcca
      661 ttaccgttca tcctgctaaa taccaatcga aaattatgca ggctgagagt gactggagtg
      721 cagcatctta ttatttttcg gtatgtgcgt taagtccgaa ttcaactttg aaaattagtg
      781 gtctattcga aaatagtttg cagggcgata gcgtattacc tgaaatatat cggcatttag
      841 gtgttgaggc gcatttcgaa aatacagtac tcgttttaca acaaaaaggc aaatgtgttg
      901 aaacattgga atttgatttt agcaattgtc cggatttagc acaaacagta gcagttactt
      961 gtgcaggatt aggggtgaag gcaatattta ccggtttaga aagtttacgc atcaaagaaa
     1021 ctgacaggac tgcagcttta caaaccgaat taaaaaagtt tgatgttgag ttttgggaag
     1081 aaggtgaggc ctggaaatta aatggaaaaa ccaatgctgc ttcagtaatt acaattaata
     1141 cttacgaaga ccatcgtatg gctatgtgtt ttgcgccatt gagtttatta catccgcaaa
     1201 ttgttattga agaaccttgg gtggtaaaaa aatcttaccc ttctttttgg gatgactttt
     1261 ctaaactagg gtttgtttct tcaattggtt gaggcacatc cggagttaat aaatcatctt
     1321 tcgacattgg tctttcacta taactccatt tgaaattttt taataagaag tcgctgagca
     1381 ctgcatcctg catgggttga aactcgctat caggcgcatt tttgaatgta atgcgatgga
     1441 attttttatc gctcagtaaa ataatgattt cactacaaaa cattttattt acgccaatat
     1501 atcgatcctt gtcatctttg ccataataaa tactttctcc gttgccaatc gcttttattt
     1561 ttaccaaatc ttcgttggaa aaataaccaa atatatttct gccttttatt tgattatata
     1621 ttaaactgtc gtcctcattg ataatgaaac cgtcagtaaa taattgaatt tcctgtaacg
     1681 ttttattacg cataatcata ttaatagtat ctgcagtaaa ctgagtgctg tcgctccaca
     1741 taataggtgc gccatacatt cttattacag aatccatatc ggcataactt aacgaatcgc
     1801 atttgccttg taaatcgctt ttataaattt ttacgttatg aaaaacaagc aatgttttgc
     1861 gctttaaact gtcctgaatg gagcggatgg tatcgccacc aataaataaa gtatcggtgt
     1921 ccataacaaa tcctgcaatg gaacctcccg ttgaaaatat ggtgccgttt atttcatcat
     1981 actcagcaaa attgctgtat tgtagcactt gttgtgaagt atcagtaaat acgatattgt
     2041 aataagcttt gccaatacct gtttcacgat tgtaataaat gctatcggct gtcagcgact
     2101 gcggtggatt atccagtttg gcatgctgat gaaatacagc tataccactg ttggtttcat
     2161 aatacccttt ttcgcaatac acagtgctct ctttattaaa tatagttgta ggaccacgaa
     2221 agattgcttt ttcggttgcc gtattatatt gtagggtatc cgcttttaat ccatattcag
     2281 gatgcgttaa aacaacatcg tttttaaaat aagcatcatt agtattcgca taataataac
     2341 ctaattggct ggttaatact gcttcaccat ctgttacttg tccaccattt atataagtag
     2401 caattttagt tgttaaatcg taactcaaat tatcggtagt gagtgtcatc gatttatctg
     2461 taatttttgc attgctatat aacttggcaa ttttggaatt tccattatag atggcagagt
     2521 cgccataggc atgaattgaa tcggcttgaa tcaagtgcac atgaccataa attttagcca
     2581 tgttttctga tttaaacaaa agggcggaat cgcaatagag aattaaatca ttttgttcga
     2641 gaataacatt attgattaac ctgtttacac tcacattctt tttttgaata atttccagat
     2701 aatcggcact acgaatttgt atttcgctgg aatctttttt tgttttaaca ggcatattgg
     2761 tttgagcttg tactacatga gcagacaaat tacaaacaac cagcaaaatg ccggcaataa
     2821 ttaacctgaa tgtcgaattt ttcataatgc ttttcgcaat cctatttacc aaaaagcttg
     2881 agtttgatgg taggatcttc agaaaacttg gcaactgctg aatctaaagc ttttaatgag
     2941 gcaagcagtt cgttgtataa ttcttcatca ttcaccaatt ttcctaaact gccatcacct
     3001 tcattaattt tttgagaaat agtattgata ttttctgcag ctaatgcaaa ttgcgtgcta
     3061 agttcctgcc attcgatggt attgagggaa tcggttactg cttttaaatt agataaaata
     3121 gatttaatat cattttcgct ttcgttcaca taattagtga tatctgatac gtcggatata
     3181 atagaatcta ttgcgccttc ttcttttata atgatattgt taacgcggtc ggcagattga
     3241 ttaaacgatt ccaaagtaag tttaatactc tgcatacttt tttgtaaatc gttttgtaca
     3301 tcttcagtaa aaatacttct caaaacagta atggcagtat ccatttgcga aagcatactt
     3361 tttactttat cgctaattgg tttgaactct gcgcttatgg tattagccaa atcagattca
     3421 atttcaccca ataaattact attaggtttt gccatttctg tagcattacc taaatatatt
     3481 tccacatatt tttcacccat caaattccct ctaatgcgcg cgcgtgagtc aattggcaca
     3541 tttacatctt ccgaaaaatt tagcactacg tttacaccgg actggtcttc ctgaagggca
     3601 actgatttta ctcttccaac ggaatatccg tcaataatta taggatcacc taccaacaat
     3661 ccgcctgcac tgccatattc tacaaaataa tcggttcctt tatgaaatat attgattcct
     3721 ttaaggtaat taaaaccaat aaacaataat gctataccta tagccgtcaa aactcctacc
     3781 ttcgattcat tagatatttt caagtgatat gtctttgagt gtgctaaatt ataaaatagt
     3841 tggcaagttg cgttcaatta ttttttcgca atagcctttg cttcttcaat ggagatacgt
     3901 tttccatcgt ttatcgctat aacaaatgca tctttataac cgttttcgcg ggcaaaagtt
     3961 tgttttttag tagcttcatc taaagtagaa taggggccta aacgatactt atacatgccg
     4021 ttttctttaa caaatgtgat gttttctttg cttagcgcgg agaatttgct gtttttttct
     4081 tccacggcac ttgcataaaa ctgaacgctg aaactcaatc ctttagtatc tccggccacg
     4141 gcaataggat tttcgacagg tgtttctttt ttgtccattt gggcttccaa ttcctgggct
     4201 ttttcagcac tcaccttacc tgaattgttt tccatatcat ttttataatc acggaatgca
     4261 taaaaaatgc tttcggcaat ggcagattg
//
LOCUS       DAORKW010000303         4285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_992_length_4285_cov_4.320804, whole genome shotgun sequence.
ACCESSION   DAORKW010000303 DAORKW010000000
VERSION     DAORKW010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_992_length_4285_cov_4.320804"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3388
                     /locus_tag="PLB46_11300"
     CDS             <1..3388
                     /locus_tag="PLB46_11300"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF77151.1"
                     /translation="VVVKYQAPLVDKGNTSTGGVVTKEDIQKIATRNVTSIAATKEGV
                     YQSDEGGGLNIKGSRGDATEYIVDGVRVSGSLKLPQDAIEQLEVITGGVDPKYGDATG
                     GFITITTRGPAKNYNGSVELASSQYLDPYGYNLASVFLTGPFIVKNKGTDSAQAKFGF
                     FLAGELEMEKDPDPSSIGNYVVKDDVLSEISTNPLRPSVSGLGFNKNSEFLTMDDLEE
                     IAYKPNTNNYGASVSTKFDYKFSRNTNVQLGLTWRKFHANTYSRNFALMNSENNPVDD
                     QNTYRGYLRFTQRFPEKRATEGEESVIGNAYYSIQVDYTKFLSTRQDADHGMNPWAYG
                     YIGAYNTYKAPVYFYTTDETTGLTGWTLVGYQDTLVEFTPGTSNPELSEYTTEFYEGS
                     AVNPSSLFDIQLGGGLLNGESASLFLSTYNMWYNNGVPWFNYSKTDQDQYSLNFGASL
                     DIKNPNSKNIGKHAIEFGFEFQQRVERFYGVNPINLWTIARQLTNKHILTLDTDNPIL
                     VIDGQEYTYEEYISTPGLYFGEFDTITYDRLLLPDDQAYFDQQLRNKLNELGYNIGEL
                     DYINIDNLPLDVFSMDLFSADELLNQGSSLVFYNGFDYLGNRSNDLISFNDFFTQEEN
                     GIKTRPIDGFRPVYTAGYIQDRFNFNDIVFRVGVRVDRYDANRKVLRDKYSLYDVYST
                     SEVDGAYNPNGAHPGNIGEDYVVYVDDFNSPSPTILGYRDEDTWYNADGQEVADPNVI
                     AVTSSTGTITPYVVDPDADIKDDNYDPNSSFEDYTPQITIMPRIAFSFPISKQQDREA
                     LFFAHYDILTQRPPTGTSTTPADYYFFLENAGALLDNPDLKPEKTIDYQVGFQQQLNS
                     SSVIKISAFYKELRDMIQIVNVPYAYPLTYTTYGNIDFGTVKGLSISYDIARRTRNLK
                     LSTSYTLQFADGTGSSATSQVNLVGAGQPNLRTIIPLSYDSRHSLKLVLDYRFEDPDG
                     SQPTWLDNAGLNVTFNARSGEPYTRQANATPTAQFGVQNRSTLDGAINGSRLPWHYRT
                     DIKLDKAFYFGLGKNDRPISMDVYVWVQNLLDAENIIAVYGYTGNALDDGYLTSAEGV
                     EAISSQVDPAAFTDLYTVKMMNPDNYSIPRRIRLGVSFDF"
     gene            3409..>4285
                     /locus_tag="PLB46_11305"
     CDS             3409..>4285
                     /locus_tag="PLB46_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77152.1"
                     /translation="MYKYKFSWILILFVFIGLNVQAKENLGITGKRTNNYAKLSAGCA
                     DATTQVDLDINNVRCRLLGAGDFWWDLDNDAKYEIPKVDPTTGAIPVSSSFAGALWIG
                     GIDAGGQLKIAAQTYRQSGNDFWPGPLDADGTIEEATCNAYDRHWKINRTDIDNFFET
                     IIEYGGVPGVSTTPALIPEGDIPAIIREWPAVGNQYATGKAGELLDVNKDLAPYVDVN
                     FDGIYSPELGEYPKIDGDQAIFWVYNDKGNIHTESGGDAIGVEIQALAFGFQTNDEIN
                     DMTFYRYKVTHYAPTP"
BASE COUNT         1316 a          780 c          829 g         1360 t
ORIGIN      
        1 cgttgtggtt aaatatcagg cgcctttagt ggacaagggt aataccagca cgggtggtgt
       61 tgttaccaaa gaagacattc aaaaaattgc aacccgtaac gttacctcta tcgcagctac
      121 aaaagaaggg gtttatcaaa gcgatgaagg tggtggattg aacattaaag gttcacgtgg
      181 cgacgcaaca gaatatattg ttgatggtgt gcgtgtatct ggttcattga aattaccaca
      241 ggatgctatc gaacaattag aagttattac cggtggtgtt gaccctaaat atggtgatgc
      301 aactggtggt ttcattacaa ttactactcg cggtccagca aaaaactata atggtagtgt
      361 cgaattagcg agttctcaat accttgatcc ttatggatat aaccttgcat cggttttctt
      421 aaccggtcct tttatagtta aaaataaagg aacagattct gctcaagcta aatttggttt
      481 cttcctggca ggtgaattgg aaatggaaaa ggaccctgat ccatcttcaa tcggaaatta
      541 tgttgttaaa gatgacgtgt taagcgaaat ctctaccaac ccactccgtc catcagttag
      601 tggtttagga tttaataaaa attctgaatt cttaaccatg gatgatttgg aagaaatcgc
      661 ctataaacca aacacgaata actatggtgc ttcggtttct acaaaattcg actacaagtt
      721 ttcaagaaat acaaacgttc aacttggttt aacctggaga aaattccatg caaatactta
      781 tagcagaaac tttgcattga tgaattcaga aaataacccg gttgatgatc aaaatactta
      841 ccgtggttac ttacgcttca ctcagcgttt ccctgaaaaa agagcaactg agggtgaaga
      901 atcagttatt ggtaatgcat attactcaat tcaagtagat tacaccaaat tcttatccac
      961 tcgtcaggat gctgaccatg gtatgaaccc ttgggcttat ggctatattg gtgcctacaa
     1021 tacttataaa gcacctgttt atttttatac cactgatgaa acaaccggct taactggttg
     1081 gactttagtt ggttaccagg atactttagt tgaattcact ccaggaactt caaatcctga
     1141 actttctgaa tacactactg agttttatga aggtagtgca gttaatccat ctagtttatt
     1201 tgatattcag ttaggtggtg gtttgttaaa tggtgaatct gcttcattgt ttttatctac
     1261 atataatatg tggtataata atggtgtgcc ttggttcaat tattctaaaa cagaccaaga
     1321 tcaatatagc ttaaacttcg gtgcttcatt agatattaaa aaccctaatt cgaaaaatat
     1381 tggtaaacat gctatcgaat ttggttttga attccaacaa cgcgttgaac gtttttatgg
     1441 tgttaacccg attaacttat ggacaattgc ccgtcagtta accaacaaac atattctcac
     1501 cttagatact gataatccaa ttttagtaat tgatggtcag gaatatactt atgaagaata
     1561 tatcagtact ccaggtttat actttggtga atttgatacc atcacttacg accgtttatt
     1621 attaccggat gatcaagctt attttgatca gcaattacgt aataaattaa atgaattagg
     1681 ttacaacatt ggtgagttag actatattaa tatagataac ctgccattag atgtattctc
     1741 aatggattta tttagcgctg atgaattatt aaatcaaggt agttcattag tattctataa
     1801 tggtttcgat tatttaggaa acagatcaaa tgatttaatt tcttttaacg acttctttac
     1861 acaagaagaa aatggcatca aaacccgtcc gatagatggt ttccgccctg tatatactgc
     1921 cggttatatc caagacaggt ttaactttaa cgacatcgta ttccgtgtag gtgtgcgtgt
     1981 tgaccgttat gatgctaacc gcaaagtact gagagataaa tattcattat atgatgttta
     2041 ttctacttca gaagtagatg gcgcttacaa cccgaatggt gctcacccgg gcaatattgg
     2101 tgaagattat gttgtttatg ttgacgactt taatagccct tcaccaacaa ttttaggtta
     2161 ccgtgatgag gatacatggt ataacgctga tggacaggaa gttgctgacc ctaacgttat
     2221 tgctgttact tcttctacag gtactatcac tccttatgtg gtggatccgg atgctgatat
     2281 taaagatgat aattacgatc ctaactcttc atttgaagat tatacgccgc aaattaccat
     2341 catgcctagg attgctttct cattccctat ttctaaacaa caagatagag aagcattatt
     2401 ctttgcacat tatgatatct taactcaacg ccctccaaca ggaactagca caactccggc
     2461 agattattat ttcttcctgg aaaatgcagg tgcattatta gataacccgg atttgaaacc
     2521 tgaaaaaact atcgattatc aggtaggttt ccaacaacag ttaaactcat catctgtaat
     2581 taaaatatct gctttctaca aagaattgcg tgatatgatt caaattgtga atgtgcctta
     2641 tgcatatccg cttacttata ctacttatgg taatattgac tttggaactg taaaaggttt
     2701 atctatatct tatgatattg ctcgtcgtac tcgtaacctt aaattatcaa ccagctatac
     2761 cttacagttt gctgacggaa caggttcaag tgcaacatct caggtaaact tagtaggtgc
     2821 tggacaacca aacttgagaa caattatacc attatcatac gattcacgtc acagcttaaa
     2881 actagtatta gactatcgtt tcgaagatcc ggatggttcg cagcctacat ggttagacaa
     2941 cgccggttta aacgtaacat ttaacgctcg ttctggtgaa ccttatactc gtcaggctaa
     3001 cgctacccca actgcacagt ttggtgttca aaaccgttca accttagacg gagcaattaa
     3061 cggttctcgt ttaccttggc attatcgtac agatattaaa ttggataaag cattctactt
     3121 tggtttaggt aaaaacgaca gacctatttc aatggatgtt tatgtttggg tacaaaattt
     3181 actcgatgct gaaaatatca ttgctgttta tggatataca ggtaatgctt tagatgatgg
     3241 ttacttaact tctgcagaag gtgttgaagc tatctcttca caagttgacc cggcagcatt
     3301 taccgattta tatacagtta aaatgatgaa cccggataac tatagtattc caagacgtat
     3361 ccgtttaggt gtttcattcg atttctaata aaagttaaac taatagatat gtataaatat
     3421 aaatttagtt ggattttaat tctgttcgtg ttcattggtt tgaatgttca ggccaaagaa
     3481 aatctcggta taacaggaaa acgaaccaat aactatgcaa aattatctgc cggttgtgcg
     3541 gatgctacaa ctcaggtgga cctcgacatc aataacgtaa gatgtcgttt attgggtgcc
     3601 ggcgattttt ggtgggacct tgataatgat gcaaaatatg aaattcctaa agttgaccca
     3661 actacaggag ctattccggt aagttcaagc tttgccggcg cattgtggat tggtggtatt
     3721 gatgcgggtg gacagcttaa aattgccgct caaacttatc gtcaaagtgg taacgacttc
     3781 tggcctggtc ctctggatgc agatggtact attgaagaag caacttgtaa cgcttatgac
     3841 cgtcactgga aaattaatag aaccgatatt gataatttct ttgaaacaat cattgaatac
     3901 ggcggtgttc caggcgtttc aacaacacct gcacttatcc ctgaaggtga tattcctgca
     3961 attatacgcg aatggcctgc agtaggtaat caatatgcta caggtaaagc cggagaattg
     4021 ttggatgtaa ataaagacct tgctccttat gtggatgtta actttgacgg tatttattcg
     4081 cctgaattag gtgaatatcc aaaaattgat ggcgaccagg ctattttctg ggtgtataac
     4141 gataaaggta atatccacac cgaatcaggt ggtgatgcta tcggcgttga aatccaggca
     4201 ttggcttttg gtttccaaac gaatgatgaa attaacgaca tgacattcta tcgttataaa
     4261 gtaactcact atgctccaac tccct
//
LOCUS       DAORKW010000304         4285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_993_length_4285_cov_3.805674, whole genome shotgun sequence.
ACCESSION   DAORKW010000304 DAORKW010000000
VERSION     DAORKW010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_993_length_4285_cov_3.805674"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /gene="nuoK"
                     /locus_tag="PLB46_11310"
     CDS             complement(<1..285)
                     /gene="nuoK"
                     /locus_tag="PLB46_11310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRF77153.1"
                     /translation="MNEISITEILFLSTALFFIGVYGLLTRRNMITMLMAVELILNSV
                     NINFVAFNKYLYPDNLDGLFFTIFIIAIAAAEAAVAIAIIIHIYRNHHSID"
     gene            complement(282..800)
                     /locus_tag="PLB46_11315"
     CDS             complement(282..800)
                     /locus_tag="PLB46_11315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRF77154.1"
                     /translation="MSAYEIIFYLLAAFILGNGILAMTTTKIFRAAIFLLFTLIGVAG
                     LYFWMQIEFVAAVQIIVYVGGIVVLIIFSIFLTQQSGLEMPKATLQRKIFSGIAALCG
                     AGLTGLLISQYGFTNNPDAVLDDSVQNIGTQMLDYEDVGFALPFEVISILLLAAMIGA
                     IVIAMKSKVEES"
     gene            complement(836..1306)
                     /locus_tag="PLB46_11320"
     CDS             complement(836..1306)
                     /locus_tag="PLB46_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRF77155.1"
                     /translation="MFITQYIKEVFTGVKSLLTGMRRAGYYFTHHKEIITQQYPNEIP
                     VMAERFKGEVTMLHDENNEHACTGCTACELACPNATIKIVTKFDITPEGKKKKAIDKF
                     VYHLELCTLCNLCIEACPTGAIKMGQNFEHSVFNRVELTKVLNKPGSKVRDDVE"
     gene            complement(1369..2445)
                     /gene="nuoH"
                     /locus_tag="PLB46_11325"
     CDS             complement(1369..2445)
                     /gene="nuoH"
                     /locus_tag="PLB46_11325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRF77156.1"
                     /translation="MISFEYIADNIDKWLNNTFSPWLALTFEFVIVGVLCIGLFAVLG
                     LVLIMMERKVSAYMQVRLGPNRVGPKGMFQTVADTLKLLVKEGLTPAGSDKFLFNLAP
                     FVVMIAAMLVLAPIAFAKNFQIWDINIGVLYVSAVSSIGVIGILMAGWASNNKYSLLG
                     AMRSGAQIVSYELSAGLSILVVVIFTGDLSISQIIESQQTGWWIFRGHIPMLIAFIIF
                     IIAVTAETNRAPFDLAEAESELTAGFHTEYSGMKFALFFLAEYVNIFIVCAIGATLFL
                     GGWMPLHIGNWEAFNSIMDWIPSSLWFVGKTFFLIFVIMWFRWTFPRLRIDQLLNLEW
                     KYLLPISMFNLLLATIIAIQGWYF"
     gene            complement(2577..3692)
                     /locus_tag="PLB46_11330"
     CDS             complement(2577..3692)
                     /locus_tag="PLB46_11330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRF77157.1"
                     /translation="MLEEIAVNDTGDLIINVGPQHPSTHGVLHLIITLNGETIKNIDP
                     NLGYIHRSIEKMCESLSYRQFIYVTSRMDYLSSHINNHACALTVEKGLQIEVPERAQY
                     IRVIMDELTRLASHQLWWGAAAMDVGALTPFFHAFREREMINEIMEETCGARLTMNYN
                     VPGGVMADIHPDFQRKTKAFLAFFKDKLPEYDELVTGNIIFQNRMKDIGIISKEDAIS
                     YGCSGPTARASGVSCDIRKTHPYSIYDKVQFDEVLETTGDSFGRYLVRMKEMRQSIHI
                     IEQLIDNIPEGDFQAKTKAVLKLPKGEFYSRVETARGELGVYIVSEGGLTPYRIKFRS
                     PGFSNLSALNHMAKGQKIGDMVAIMATLDLVIPDIDR"
     gene            complement(3735..4193)
                     /locus_tag="PLB46_11335"
     CDS             complement(3735..4193)
                     /locus_tag="PLB46_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRF77158.1"
                     /translation="MMEREALQAQITAINANVTFEEGGEMLNAFVDPKECRDLLQTLH
                     NMDDLAFDYMFCLTCVDWKTHLTMVYHLESTKHRHVVVIKSKLDRENPEIDTMSDIWR
                     TAELLEREVYELFGVRFINHPDLRKLLLSDEWEGWPLRKDYEDPINMIKL"
BASE COUNT         1371 a          880 c          695 g         1339 t
ORIGIN      
        1 atcaatagaa tgatggtttc tataaatatg gattataata gcaatagcca cagcagcttc
       61 agcggcagca atggcaataa taaatatcgt aaaaaataat ccgtcaagat tatcaggata
      121 caagtatttg ttaaacgcaa caaagttgat atttacgcta ttgagaatta actcaactgc
      181 cataagcatg gtaatcatgt tgcgacgtgt aagcaggcca taaacgccga taaaaaacag
      241 cgctgtgctt aaaaataaaa tctcagtgat acttatttcg ttcatgattc ttcaacttta
      301 gatttcatgg caataactat tgcgccaatc atagcagcca ataatagaat actgattact
      361 tcaaatggta atgcaaatcc aacatcttca taatccaaca tttgggttcc tatgttttgc
      421 actgaatcgt ccaatacggc atcaggatta ttagtaaaac catattgact tattaataaa
      481 cctgttaagc cggcaccaca tagtgcagcg attcctgaaa atatttttct ttgtaaagta
      541 gccttaggca tttctaagcc tgattgctgt gtgaggaaaa tagaaaaaat tatcagtaca
      601 acaataccac ctacataaac aataatttgc actgcggcaa caaattcaat ttgcatccaa
      661 aaatataaac cggcaacacc aattaaggtg aataataaaa atatggcagc tctaaatatt
      721 ttagtggttg tcatagctaa aattccattg cccagaataa aagctgctaa aaggtaaaat
      781 atgatttcgt aagcgctcaa ggtataagtt gtgttatttt taaaatagtt attgtttatt
      841 ccacatcatc tctcacttta ctacccggtt tgtttaatac tttagttaac tcgacacggt
      901 taaatacact gtgttcgaaa ttctgtccca ttttgattgc tcctgtagga catgcttcaa
      961 tacacaagtt gcataatgtg catagttcga ggtgataaac aaacttatcg atagcttttt
     1021 tcttttttcc ttcaggtgta atatcgaatt tagttacaat tttaattgta gcatttgggc
     1081 aagccaattc acaagcagtg caaccggtgc aggcatgttc gttgttttca tcgtgcaaca
     1141 tggttacttc acctttaaat cgttcagcca taaccggtat ttcgttcggg tattgctggg
     1201 tgattatttc tttatggtgt gtaaaataat atcccgctct gcgcatgcca gtcagaagcg
     1261 attttactcc ggtgaaaact tcttttatat attgcgttat aaacataagt tcaaaaatta
     1321 actacaaatt caattcactc aaatttgttt tgttgcattt gcaactcatt aaaaatacca
     1381 accttgtata gcaattatag tagccaacaa taaattaaac attgaaatag gcaacaaata
     1441 tttccattca agattgagta attgatcaat gcgtaaacga gggaatgtcc aacggaacca
     1501 cataataacg aaaatgagga aaaaggtttt tcctacaaac cacagcgatg atggaatcca
     1561 gtccataatg ctgttgaacg cttcccaatt accgatatgc aaaggcatcc agccaccaag
     1621 aaataaggtg gcgccgatag cacatacaat aaatatattt acatattctg ctaaaaagaa
     1681 taatgcgaat ttcattcccg aatactctgt gtgaaatcct gcggttaatt ccgattctgc
     1741 ctcagctaaa tcgaaaggtg cacgattcgt ttctgctgtt acggcaataa taaaaataat
     1801 aaaggctatt aacattggga tatgccctct gaaaatccac caacctgttt gttgcgattc
     1861 gataatttgt gatatggata aatcgccggt gaaaattaca acaaccaata tggataaacc
     1921 tgcacttaat tcataactca caatttgtgc gccgcttcgc attgcaccta gtaaagaata
     1981 tttattatta cttgcccaac cggccattaa aatacctatt acaccaattg atgaaacagc
     2041 acttacatat aaaacaccaa tattaatatc ccaaatttga aagtttttgg cgaaagcaat
     2101 tggtgctaaa accaacatgg cggcaatcat tacaacaaat ggtgccaggt taaataaaaa
     2161 tttatcggaa cctgcagggg taagcccttc tttgactaat aatttgagcg tatcagcaac
     2221 agtttggaac atcccttttg gcccgacacg attgggtcct aaacgtacct gcatataagc
     2281 cgatactttg cgttccatca taattaatac taaacccaaa acggcaaata agccaatgca
     2341 caaaacacca acaataacaa actcaaaagt taatgcaagc catgggctaa atgtattatt
     2401 taaccactta tcaatattat ctgcaatgta ttcgaaactt atcaaagcga atgctatttt
     2461 attttatttc aatcaatcca attcaaaaaa atctctttca atcctcaatc ttgttcaatc
     2521 cccaaatcat tcaattttgt tcaatcaaca aatcttgttc aatccacaaa tccctctcac
     2581 ctgtcaatat ccggaatcac caaatcaagc gtagccataa tagccaccat atcgccaatt
     2641 ttttgacctt tggccatatg atttaatgca ctcaaattgg aaaaacccgg tgaacggaat
     2701 tttattctat aaggtgttaa acctccttca ctcacaatgt acacccctaa ttcccctctt
     2761 gctgtttcaa cacgtgaata aaattctcct ttaggtaatt ttaaaactgc ttttgtttta
     2821 gcctgaaaat ctccttcagg aatattatct attaactgtt caataatatg aatggattga
     2881 cgcatctcct tcatcctcac taaatatctg ccaaaactgt cgccggtagt ttccagcact
     2941 tcgtcaaatt gtactttatc gtaaatactg taaggatgtg tttttctgat atcacaactc
     3001 actccacttg ctcttgcagt tggacctgaa cagccataag aaatggcatc ttcttttgaa
     3061 atgataccga tatctttcat cctgttttgg aaaatgatat tgccggttac caattcatca
     3121 tattcaggta acttatcttt aaaaaaggct aaaaatgctt tggttttgcg ctgaaaatcc
     3181 ggatgaatat ctgccattac gccacctgga acattataat tcatagtaag gcgtgcacca
     3241 caggtttctt ccattatttc attaatcatt tctctttcgc gaaatgcgtg aaaaaatggt
     3301 gttaaagcac caacatccat agcggcagca ccccaccaca attgatgtga agccaatcgg
     3361 gtaagctcat ccataattac ccggatatat tgcgcgcgct cagggacttc aatttgtaaa
     3421 cctttttcta cggtcaaagc acatgcgtgg ttgttgatgt gagatgacaa gtagtccatc
     3481 cttgatgtta cataaataaa ctgtctgtaa ctcaagcttt cgcacatttt ttcgatggag
     3541 cgatggatat aacccagatt tgggtcgatg tttttaatgg tttctccatt taaagtgata
     3601 atcaaatgca acacaccatg ggtggaaggg tgctgtggtc cgacattgat tatcagatca
     3661 ccggtatcat ttactgcaat ttcttctaac attgtttcta accgggtata atttatttaa
     3721 aaattgaaat agtgttacaa cttaatcatg ttgataggat cttcgtaatc cttgcgcaac
     3781 ggccaacctt cccattcgtc agaaagtaat aattttctaa ggtcagggtg attgataaac
     3841 cgcacaccaa ataattcgta cacctcgcgt tccagcaatt ctgcggttcg ccaaatgtca
     3901 ctcattgtat ctatttcagg gttttctctg tctaattttg acttaattac tacaacatgt
     3961 ctatgttttg tcgattccag atgatacacc atagtaagat gtgttttcca gtccacacag
     4021 gtcaggcaaa acatataatc aaaagccagg tcgtccatat tatgcagggt ttgcagtaaa
     4081 tcacgacatt cttttgggtc cacaaaggca tttagcattt cgccaccttc ttcgaaggta
     4141 acattcgcat ttattgcggt aatttgggct tgtaatgctt cgcgttccat cattcagtta
     4201 ctttatcgta aaatttctct attaccattt tttcgcggtt gccaccggat tttatcttgg
     4261 tttgcagcat catcagcgca tgcat
//
LOCUS       DAORKW010000305         4284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_994_length_4284_cov_4.811539, whole genome shotgun sequence.
ACCESSION   DAORKW010000305 DAORKW010000000
VERSION     DAORKW010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_4284_cov_4.811539"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1442)
                     /gene="ffh"
                     /locus_tag="PLB46_11340"
     CDS             complement(102..1442)
                     /gene="ffh"
                     /locus_tag="PLB46_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRF77159.1"
                     /translation="MFENLSDRLEGAFKSLKGQGKISEINIATTVKEIRRALVEADVN
                     YKIAKEFTDTVKDKALGQEVLTSVSPGQLMVKIVQDELTALMGGAQVDINIKGSPAII
                     LIAGLQGSGKTTFSAKLANHIKTKLGRNPMLAACDIYRPAAINQLQVLGEQIGVHVYA
                     DIENKNAVDIAKKAINYAKTNNHNVVIVDTAGRLAVDEEMMDEITRLKEALNPSETLF
                     VVDSMTGQDAVNTAKAFNDRLNFDGVVLTKLDGDTRGGAALSIKYVVQKPIKFVSRGE
                     KMDTLELFYPDRMAQRILGMGDIVSFVEKAQEQYDQEKAEELQRKIRKNQFDFEDFLQ
                     QLKQIKKMGNMKDLLGMIPGIGKQIKDMDIDEKQFSKLEAMIYSMTPEERNNPDLMTG
                     TRRQRIAAGSGTTIQDVNQFMKQFEEMRKMMKMMNKMGGGAKAARALGGMMGKR"
     gene            complement(1726..3174)
                     /locus_tag="PLB46_11345"
     CDS             complement(1726..3174)
                     /locus_tag="PLB46_11345"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRF77160.1"
                     /translation="MNNTYELKLKEWNMKEKAAVELSSITTHLWYDKSVELIIFRRNL
                     VDARASEILNHHLYAQRFLNRPLTIEVSLALTKAINELDLAPSRIDLGRLGSEWLSEG
                     SNYANELEFVKDKLKEFIGQDKMVLKPKDVVLYGFGRIGRIAARIIINEAGKGEQLRL
                     RAIVTRSNSDEDIIKRASLLRRDSIHGKFRGTIVEDLENKALIINGHTVYMIAADKPE
                     SIDYNAFGIDDALIIDNTGIARDRANLSKHLEAKGVSHVLLTAPGKGDIPNIVHGINH
                     ENFDPAKEKIFSAASCTTNAVVPILYTMEKMFGIDHGHLETVHSYTNDQNLLDNYHKK
                     SRRGRSAPMNMVITETGAAGAVAKVLPDLAGKFTGNAIRVPTPDVSLAIMNLNLKKEV
                     TIEEVNNVLREAASFGQLVEQIDYSISLEIVSSDCIGNSHCVIVDAPATIVSKDGKSV
                     VLYCWYDNEFGYTKQVVRLSKYLAGVIRLRYY"
     gene            3405..3788
                     /locus_tag="PLB46_11350"
     CDS             3405..3788
                     /locus_tag="PLB46_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HRF77161.1"
                     /translation="MSKKKQFTIEYIIKSSPLVLYNCLITATGLQQWFADTVDNDGNT
                     FYFTWNGETEIAELISTEEDDYIRFRWEYQDEDEYFEFRIDKTEITNDTILFVTDFAD
                     DREVDAQRRLWDAQIHNLIKRIGAS"
     gene            complement(3783..>4284)
                     /locus_tag="PLB46_11355"
     CDS             complement(3783..>4284)
                     /locus_tag="PLB46_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77162.1"
                     /translation="FENIRLALKDSAQHKQFLFPDGTDYTTGKIAQGENYLGYPWVML
                     DFPRCFKKNAMFAYRTMFWYGHYFSVSLVLSGDHLSTLLPGLATKLRRTPDGLLFATH
                     GDAWCHDLDASTFLPANQLSTVAIMEHVERHDYIKLSIKIDEPDMKKLSERVVAHYEM
                     LLQLLL"
BASE COUNT         1238 a          981 c          704 g         1361 t
ORIGIN      
        1 tacctttact cctgaccacc gtctacgacg gtggttttgt tgcctaaata aaaaaaggcc
       61 accctcaggc agcctttaat tcatatcgtt aaatcgtaat attatctttt tcccatcata
      121 ccacccaatg ctcttgcagc tttggcgccg ccgcccattt tgttcatcat tttcatcatc
      181 ttgcgcatct cttcaaattg tttcataaac tgatttacat cctgaatagt agtgccacta
      241 cctgcagcaa tgcgttggcg acgagtgcct gtcatcaggt cgggattgtt acgctcctct
      301 ggtgtcatac tgtaaatcat ggcctcgagt ttcgaaaatt gtttttcatc gatgtccatg
      361 tcttttattt gtttgcctat accgggtatc atgcctaata aatctttcat gttacccatc
      421 tttttaattt gcttcaactg ctgtaaaaaa tcctcgaaat caaattgatt tttacgaatt
      481 tttctttgca attcctcagc cttctcctgg tcatattgct cctgggcttt ttctacaaac
      541 gatacgatat cgcccattcc caaaatacgc tgagccatac ggtcaggata aaacaattcg
      601 agggtatcca ttttttcgcc gcgactcaca aacttaatcg gcttctgcac tacatattta
      661 atcgacaatg ccgcaccacc gcgtgtatca ccatctaatt tggttaatac cacaccgtca
      721 aaattcaaac ggtcgttgaa tgcttttgct gtattaactg catcctgacc ggtcatgcta
      781 tccaccacaa ataaagtctc tgaaggattc aacgcttctt ttaatcgcgt aatttcatcc
      841 atcatttctt catccacagc taaacgaccg gcagtatcta caattacaac attgtgatta
      901 tttgttttgg cataattgat ggctttttta gcaatatcaa cagcattttt attttcaata
      961 tcggcatata catgtacacc aatttgttca cctaaaacct gtaactgatt tatcgcagct
     1021 ggacgataaa tatcacatgc cgctaacatc ggattgcgtc ccagttttgt tttaatgtga
     1081 ttagctaatt tggcactgaa agtagtttta cccgaacctt gcaaaccggc aattaaaatg
     1141 atagcaggcg aacctttaat attaatatcc acctgtgcac cacccattaa tgcagtaagt
     1201 tcatcctgca caattttcac cattaattgt cccggcgaaa cagaagtaag cacttcctga
     1261 cccaatgcct tatcctttac cgtatcggta aattctttgg caattttgta attcacatca
     1321 gcctccacta atgcacgacg aatttccttc accgtagtgg caatgttaat ttcagaaatt
     1381 tttccctgac cttttaaact cttaaaggcg ccttctaaac gatccgataa attttcaaac
     1441 atagatttat tttaattagc aaatttgtca attagcaaat tagctaatgg ttactttttc
     1501 gccacacaca caatttttct tcctcctcac atttccaatt agttcgccgc gacgaactaa
     1561 tgttgcattt tattattcat tcaaatgtgg agcacaacat ttcaatcaca ctccaaattc
     1621 acatttgcta attagctaat ttgctaatca gctaatccac tgctccgcat atccaatcac
     1681 actccgaacc acatttgcta attagctaat ttgctaatca gccaattaat aataccgcaa
     1741 cctaatcacc ccggccaaat atttcgataa acgaacaact tgtttagtat agccaaactc
     1801 attgtcgtac cagcaatata ataccacact cttaccatct ttagaaacta ttgttgcagg
     1861 agcatctaca ataacacaat ggctattgcc gatacaatcg ctgctcacaa tttctaaact
     1921 gatgctgtaa tctatttgtt caaccaactg accaaatgaa gcagcttctc ttaaaacgtt
     1981 gttcacttct tcgatggtta cttctttttt caaatttaaa ttcataatcg caagcgacac
     2041 atccggtgtt gggacacgaa ttgcgttgcc tgtgaatttt ccagctaaat ctggtaatac
     2101 ttttgctact gcacctgcag cacctgtttc ggtaattacc atgttcattg gtgctgaacg
     2161 acctctgcgt gattttttgt ggtagttatc gagtaagttc tgatcgttcg tataactatg
     2221 cactgtttct aagtgaccat gatctatacc aaacattttt tccatggtat ataaaatcgg
     2281 tacaacagca ttggtagtac aacttgctgc agaaaatatt ttttcttttg caggatcaaa
     2341 attttcgtga ttaatgccgt gaacaatatt cggaatatca cctttgcccg gagcagttaa
     2401 taatacatga ctaactcctt ttgcttctaa gtgtttactc aaatttgcgc ggtcgcgggc
     2461 aatacctgta ttatctataa ttaatgcatc atcaattcca aatgcattgt aatcaatact
     2521 ttcaggttta tcggcagcta tcatataaac agtatgaccg tttataatca gtgctttatt
     2581 ttctaaatcc tcaacaattg ttccgcggaa ttttccgtga atagaatcgc ggcgtaataa
     2641 acttgcgcgt ttaataatat cctcatcgga attagaacgt gttacaatag cacgtaaacg
     2701 caattgttca ccctttcctg cttcattaat aataatacgt gcagcaatac ggccaatacg
     2761 accaaaacca tataacacta catcttttgg ttttaatacc attttatcct gaccgataaa
     2821 ttctttcaat ttatctttca caaattcaag ttcattggca tagttgctgc cttcgctcaa
     2881 ccattcgctg cctaaacgac ctaaatcgat gcgactagga gctaaatcaa gttcattaat
     2941 cgctttcgtt aacgccaatg acacttcgat agtcagggga cgatttaaga aacgttgtgc
     3001 atacaagtga tggttcaaaa tttcgctggc acgtgcatct accaaattgc ggcggaaaat
     3061 aatcagctcc accgatttgt cgtaccataa gtgggtagta atactgctaa gttcaaccgc
     3121 agccttttct ttcatgttcc actctttgag cttcaattca taagtgttgt tcataatatt
     3181 gtaaagtatg gtttaaggtt agtttttcag gttgcaaagg taatgaatgc cgtgcagagc
     3241 tccgttactt ttgtgaaaaa tcacaataag ggtcatttaa aacctcaatg tttacagggg
     3301 ttttatcact atccccaaaa aaactttgct aaaaattttg tatcctttga aattatatta
     3361 tacttgcaac attatagcca aacccaagtt aggctttcag taacatgtcg aaaaagaaac
     3421 aattcacaat agaatacatc ataaaatcga gcccgttggt cctttacaac tgcctcatta
     3481 ccgcaaccgg gctccaacag tggttcgccg atacggtaga taatgatggt aatacctttt
     3541 attttacctg gaacggagaa accgaaattg ccgagctcat cagcactgaa gaagatgatt
     3601 acattcgctt ccgttgggaa tatcaggacg aagatgaata cttcgaattc agaatcgata
     3661 aaacagaaat cacgaacgat accatcctgt ttgtcaccga ttttgccgat gaccgtgaag
     3721 ttgacgccca acgccgcctt tgggatgcgc aaatccacaa tctcattaaa cgtatcggcg
     3781 cttcataata acagctgcaa caacatttca tagtgcgcaa ctacccgctc actaagcttt
     3841 ttcatgtccg gctcatctat ttttatactc agtttaatat aatcgtgtcg ttctacatgt
     3901 tccattatcg ctactgttga aagttgatta gcggggagaa atgtacttgc atctaagtca
     3961 tgacaccagg catctccgtg ggttgcgaat aacaaaccat ccggtgtacg ccgtagtttt
     4021 gtggccaagc ctggcagcaa ggtggaaaga tggtctccgg atagcactaa actcacacta
     4081 aaataatgcc cataccaaaa catggtgcga taggcaaaca tggcattttt tttgaaacat
     4141 cgcggaaagt cgagcattac ccaggggtaa cccagataat tttcgccctg agctattttt
     4201 ccggttgtgt aatctgtccc atccggaaac agaaattgtt tgtgttgagc actatctttg
     4261 agcgccaaac gaatattttc aaat
//
LOCUS       DAORKW010000306         4283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_995_length_4283_cov_4.895932, whole genome shotgun sequence.
ACCESSION   DAORKW010000306 DAORKW010000000
VERSION     DAORKW010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_995_length_4283_cov_4.895932"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLB46_11360"
     CDS             complement(<1..315)
                     /locus_tag="PLB46_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF77163.1"
                     /translation="MQYKFSNTQLPRIVIIGGGFAGLTMVKKLNNLPYRVTLIDKNNY
                     HNFQPLMYQIASAGLTAESIAFPLRRKIGKYPNIIFRLAEVTSIDKQHKTVHTTIGEI
                     AYD"
     gene            443..1651
                     /locus_tag="PLB46_11365"
     CDS             443..1651
                     /locus_tag="PLB46_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF77164.1"
                     /translation="MAKTTKKTGFGSLCVQPGKTDKIILPHNEPIYATTAYTFESTDH
                     ALSLFDDHHKGYVYSRWGNPTVTMAEEKIAAMETFGSEHKAKAQLFSSGMAAIAAVLL
                     SCAKAGDTILTQNQLYGTTDELMQKQLRDWNINITRCDLNDLKAVTTILKNNKQVKLI
                     YIETPSNPMMEIFDIQSIAAIAKKYGCRVAVDNTFSSPYCQRPLLLGADFSVHSATKY
                     LNGHGSGLGGVVIGLDIPFMKNEIWNKVKLLGANSNAFDSWLLLQGLKTLELRMERHC
                     ENAKKVAAFLSANKYVSQVNYCGNKQHPQKNIIKKQMLAHSGMLSFELKGGLKAGIKL
                     MNKVKVCKMVTSLGTLDTLIQHPASMTHVNVPRDRRVAAGITDGLVRLSVGIENVQDI
                     IDDLSQGLGK"
     gene            complement(1699..2574)
                     /gene="fabD"
                     /locus_tag="PLB46_11370"
     CDS             complement(1699..2574)
                     /gene="fabD"
                     /locus_tag="PLB46_11370"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRF77165.1"
                     /translation="MKKAYVFPGQGSQFPGMGKDLYANNPAAKMLFDEADAILGFAIT
                     DIMFNGTEEALKQTRVTQPAIFLHSVILASTIENFSPDMVAGHSLGEFSALVANKTLS
                     FKDGLQLVYKRAMAMQKACEMQPSTMAAVLGLADDIVERICNETEGVVVAANYNCPGQ
                     LVISGEIEAVNKACEALKAAGAKRALLLPVGGAFHSPLMEPARIELYEAINSTHFSNP
                     VCPVYQNVTAQAVADPADIKNNLIAQLTAPVKWTQSVLQMTADGATHFVEVGPGKVLQ
                     GLVKKISPTVEAASC"
     gene            complement(2605..3039)
                     /locus_tag="PLB46_11375"
     CDS             complement(2605..3039)
                     /locus_tag="PLB46_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77166.1"
                     /translation="MKNIQMVLAMLLIIGSTSLNAQSKKYKIEGTKCQRVEIIDAGEL
                     YSTYELNEGEIEMMKIAGLSNYLIDEIKGNHTEDSWPEKLGDLDARIADPDKVKSYVV
                     YKVIALEDKIILVAPLKYNKDKDSGWALSRDVFFVMSAKAIK"
     gene            complement(3081..3650)
                     /gene="folE"
                     /locus_tag="PLB46_11380"
     CDS             complement(3081..3650)
                     /gene="folE"
                     /locus_tag="PLB46_11380"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRF77167.1"
                     /translation="MDKIAALTENYKSIINHLGEDPEREGLQKTPERIAKAMQFLTQG
                     YDQDAEAVVRSAMFTEECSEMVIVKDIEIYSMCEHHMLPFFGRAHVAYIPNGKIVGLS
                     KIARVVDIYARRLQVQERLTQQVLHCLDNTLQPHGVAVVIEASHLCMMMRGVQKQNSV
                     TTTSAFTGQFQTMQTRNEFINLISARLSK"
     gene            complement(3717..4124)
                     /locus_tag="PLB46_11385"
     CDS             complement(3717..4124)
                     /locus_tag="PLB46_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRF77168.1"
                     /translation="MVYLTRRETFNAAHRLYNNKWSEEKNFEVFGKCANKNWHGHNYQ
                     LFVTVKGNPDPDTGFIMDAKKLSDLIKKEIVELFDHKNLNTDTPYFKEVQPSTENFCK
                     VIWKLIEPLITGGVLHCIKLQETENIAAEYYGE"
BASE COUNT         1322 a          794 c          777 g         1390 t
ORIGIN      
        1 atcataagca atttcaccaa tagttgtatg aacagtttta tgttgtttat caattgaggt
       61 aacttcagct aatcgaaaaa taatattggg atacttacca atttttctcc gcaagggaaa
      121 ggctatgctt tctgctgtta atcccgcaga agcaatttga tacatgagcg gctgaaagtt
      181 gtgataatta tttttgtcta ttaacgttac acggtagggt aggttattaa gtttttttac
      241 catggttaac cctgcaaatc caccgcctat tattacaatg cgagggagtt gagtattact
      301 gaatttatat tgcattgcct gagtgttttg tttataagca caacccaaaa ttctgcaatt
      361 ctgttcagtt tatcctaatt atatctgcgc aattaactaa ttgaaaataa attagctttg
      421 tgatgcaaaa aatccattaa atatggcaaa aactacaaaa aagacaggct ttggttcatt
      481 atgcgttcaa ccgggtaaaa ctgataaaat tatcctaccg cataacgaac ctatatatgc
      541 cactactgcc tatacatttg agtcaaccga ccatgcattg agtttatttg acgaccatca
      601 taaaggttat gtgtattcgc gttggggcaa tccaactgta actatggcag aggagaaaat
      661 tgccgctatg gaaacctttg gcagcgaaca caaagcgaaa gcgcaattat ttagtagtgg
      721 aatggcagct attgctgccg tattgttatc atgtgctaag gcaggagata ccattcttac
      781 ccaaaaccag ctatatggta ccactgacga actgatgcaa aaacaattac gcgattggaa
      841 tattaatatt acgcgttgcg atttaaatga tttaaaagcc gtaacaacaa ttttaaaaaa
      901 caataaacag gtaaaattga tatatatcga aacaccatca aatcctatga tggaaatatt
      961 tgatattcaa tcaatagctg caatagcaaa aaaatatggc tgtcgtgttg ctgtagataa
     1021 tactttttca tctccttatt gtcagcgacc actgttatta ggtgccgatt tctcagtgca
     1081 ttctgcaact aaatacctta atggtcacgg cagtggatta ggaggggttg ttattggctt
     1141 ggatattcct tttatgaaaa atgagatatg gaataaagta aaattattag gagctaattc
     1201 aaacgcattc gatagttggc tattattaca aggactcaaa acgcttgaat tgcgaatgga
     1261 acgtcattgc gaaaatgcta aaaaagtggc ggcgtttctc agtgccaata aatacgtttc
     1321 tcaagtaaat tattgtggga ataaacaaca tcctcaaaag aatattatca aaaaacaaat
     1381 gctagctcac agtggaatgt tgagctttga attaaaaggg ggactaaaag ccggtattaa
     1441 attgatgaat aaagtaaagg tttgtaaaat ggttacatcg ctgggcactt tagatacatt
     1501 aattcaacac cctgcgagta tgacacacgt aaatgttccg cgcgacagga gagtggctgc
     1561 aggtataacc gatggtctgg tgcgtttaag tgtaggtatt gaaaatgtgc aggatattat
     1621 tgatgattta tcgcaagggt taggcaaata aaaaagggct gtaaaaatta caaccctttg
     1681 aatttatatt ttcatgtttt agcagctcgc agcttcaact gtcggactga ttttttttac
     1741 caatccctgt aatactttac cggggccaac ttcaacaaaa tgtgtagcac catcagctgt
     1801 catttgtaat acactttgag tccattttac cggagcagtt aattgagcaa ttaaattatt
     1861 ttttatatct gcagggtcag caacagcttg tgcagttaca ttttgataaa cagggcaaac
     1921 aggattacta aaatgcgtgc tgttgattgc ttcatataat tctatccgtg caggttccat
     1981 caacggtgaa tgaaaagcac caccaacagg taataataat gcacgttttg cacctgctgc
     2041 ttttaatgct tcacatgctt tatttacagc ctcgatttct cctgaaatta caagttgacc
     2101 cgggcaatta taatttgccg cgacaacaac tccttcggtt tcgttacaaa tacgttcaac
     2161 aatatcatca gccaaaccta atactgcagc catagttgaa ggttgcattt cgcaagcttt
     2221 ttgcatggcc attgcgcgtt tatacactaa ttgtaagcca tctttaaatg ataacgtttt
     2281 attggcaact aatgcagaaa attcccctaa agaatgtccg gcaaccatat ccggtgaaaa
     2341 attttcaata gtagaagcaa gaattaccga atgtaaaaaa atggccggtt gtgtaactct
     2401 ggtttgtttt aatgcttctt ccgtaccatt aaacatgata tctgttatag caaatcctaa
     2461 aatagcatcc gcttcgtcaa acagcatttt tgcagcagga ttatttgcgt acaaatcttt
     2521 acccattccc ggaaattgag aaccttgacc ggggaaaaca taagcttttt tcattgatta
     2581 ttaaaattga aagtggttga ttatttattt tattgcttta gcactcataa caaaaaaaac
     2641 atcacgcgac aatgcccaac cactgtcttt gtctttatta tattttaaag gcgctactaa
     2701 aataatttta tcttctagtg caataacttt atacactacg taagatttta ctttatcagg
     2761 gtctgcaatg cgggcatcta aatcacctaa tttttcaggc caggaatctt cggtatgatt
     2821 accctttatt tcatcaatta aatagttgga aaggccggcg attttcatca tctcaatttc
     2881 accttcattt aattcataag tactatacaa ttcacctgca tcaattattt caacgcgttg
     2941 acatttggtg ccttcaattt tgtatttttt cgattgtgcg tttagactgg tactgcctat
     3001 gattaaaagc atagccaaaa ccatttgtat gtttttcata tcaataagtt taggctgtaa
     3061 atgtaaataa taatcggaaa ttatttactc aaacgggctg aaatcaggtt gatgaactcg
     3121 ttcctggttt gcatagtttg aaactgaccg gtaaatgctg aagtggttgt aacagaattt
     3181 tgtttttgaa caccacgcat catcatacat aaatgactag cttcaataac aacggctaca
     3241 ccatgcggtt gaagtgtgtt gtcaaggcaa tgtagtactt gttgcgttaa acgttcttgc
     3301 acctgcaatc tcctggcata aatgtcgaca actcttgcaa ttttacttaa tccaacaatt
     3361 tttccattag gtatataagc aacgtgagca cgtccaaaaa atggcagcat atggtgctca
     3421 cacatagaat aaatttcaat atcctttaca attaccattt cactgcactc ttcagtaaac
     3481 atggcgctac gcacaactgc ttccgcatcc tgatcataac cttgcgttaa aaattgcatg
     3541 gctttggcaa tacgttcagg ggttttttgt aatccttcac gttcagggtc ttcccccaaa
     3601 tgattaataa tcgatttata attttcggtg agtgcagcta ttttatccat tccgtattgt
     3661 tctacttttt tatagaactg acctgttggg ttgttatttt ccattattga gttggtttat
     3721 tcaccgtaat actccgcagc aatattttcg gtttcctgaa gttttatgca gtgtaaaacg
     3781 ccgcctgtaa ttaatggttc aattaacttc caaattactt tacaaaaatt ttctgtactg
     3841 ggctgaacct ctttaaaata gggtgtatca gtgtttagat ttttgtgatc aaacaactct
     3901 acaatttctt ttttgataag gtcgctcagt tttttggcat ccataataaa ccccgtatca
     3961 gggtccggat tgccttttac agtcacaaaa agctgataat tatgtccatg ccagttctta
     4021 ttggcgcatt tgccaaaaac ttcgaaattc ttttcttccg accatttatt gttatacagc
     4081 cgatgggcag cgttaaacgt ttcacgtctg gtaagataaa ccatggtgca aagataacac
     4141 tctatgtgtt ttaagacgat aaaatggtga ttctgttccg gttaggaagt aaaactaggg
     4201 tttaagcagc taaaatggcc acttttttag ggtctttaga gtcaaccttt acctgcagag
     4261 ttctgccttt ttgataatgg cgc
//
LOCUS       DAORKW010000307         4279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_998_length_4279_cov_4.019413, whole genome shotgun sequence.
ACCESSION   DAORKW010000307 DAORKW010000000
VERSION     DAORKW010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_998_length_4279_cov_4.019413"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLB46_11390"
     CDS             <1..239
                     /locus_tag="PLB46_11390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRF77169.1"
                     /translation="NSIKSGQPVTYPVNDFIKGWTEGLKMMKEGDKWNLYVPSELGYG
                     PGGFQGFIPPNATLIFEVELLKVTKGGAAPKMNK"
     gene            408..1706
                     /locus_tag="PLB46_11395"
     CDS             408..1706
                     /locus_tag="PLB46_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF77170.1"
                     /translation="MKKRFLGLVLIALTLSSFGQTIAITEINYNSDPYNKSNDWVELH
                     NYGGSAVDISNWKLKDETPFNSFTIPAGTVLDANEYVVLVENIDTFLMVHGGVTNYIG
                     EFAFGFDNTFGTVKIENSSGTVIKAVPYIDSIPWPKGCDGYGPTLQIINEEASESNPA
                     NWRSGCVLGTPGEAYADCNYDIVVSEINYNSLLAYNPGDWIEILNRGNADKDISGWIL
                     RDGKTDNIFVIPAGNVLSAGERLVIADSLESFTVKFPTVGNVIGEPPFSFSNGGDAVR
                     LYTPSGKIQYSVRYKDSAPWPLDPDGTGFTLELVDEPGNPNDGVAWVAGCLYGSPGNP
                     FIVPCPNAVNNVIPNELTIGPNPFTDYLTIIIDATVQAQSVTIMNTLGEQIITLPTYN
                     NAVTWNSTDLQGNTVSDGIYFVVVTDNNGNTITAKVLKAK"
     gene            complement(1772..3040)
                     /locus_tag="PLB46_11400"
     CDS             complement(1772..3040)
                     /locus_tag="PLB46_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77171.1"
                     /translation="MKFPVCLIVLFCSTVALSAQQIESTSASDSVNDNFLNVYLDGVY
                     QYHEYIKTEIPYVNYVRDRFLADVHLLITGQTTGSGGTDYAILFLGQLSYAGKNDTLH
                     FFDNTNNTDDERRSGIVSYMKMGLMPFIAKKSANFPVSIASNISTTTNEKQVDDKWNG
                     WVFNVGGYGNLSLTSNSEYYNLSGDLSIDKITEEWKLSFSLGAYYNTSNFDFGDGNKY
                     RSFNNGTYATGLIANSISEHWSVGGYVDYFSSTYSNYDADFSLYPAIEYNVFPYSKSN
                     TKLLTITYKAGPDYAIYTDTTVFNKKEELLLRERLEVELSLIQKWGSVSFGLNGTNYF
                     HDFSKNSAGLSASINWSIVEGLSFNTFFNYNLINDQLNLPKGELTDEEILLQLTERQT
                     DFSFYTYFGLTYTFGSIYNNVVNPRFNNSF"
     gene            complement(3071..3541)
                     /locus_tag="PLB46_11405"
     CDS             complement(3071..3541)
                     /locus_tag="PLB46_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77172.1"
                     /translation="MQNFSPQSKVWVYQSSRPFTESEIVELNNKLKHFTVQWTAHNKQ
                     LLASGNVMYDRFIVLMVDESHTMASGCSIDTSVHFIKQLEQDYTIDLFNRTIVNMEIN
                     GIIQTISLDELSTKFKSGEINASKMVFDPLVQQKRDFDKGFKIPLAESWMMNLI"
     gene            complement(3603..>4279)
                     /locus_tag="PLB46_11410"
     CDS             complement(3603..>4279)
                     /locus_tag="PLB46_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRF77173.1"
                     /translation="AFVRILNSIQIKFAVLGTEEGCTGDPARRAGNEFLFQMLAANNI
                     AVMNGYGIKKIVTTCPHCFNILKNEYPELGGNYEVMHHTSFLQSLIDEGKLTVQGGAF
                     KGKRITFHDSCYLGRANQIYEAPRSVLEKLDGALTEMKSCKSKGLCCGAGGAQMWKEA
                     EPGNKEINIERTEQALEVKPDIIASACPFCNTMMTDGVKIKNKEDEVKVFDVAELIAM
                     ANQLMA"
BASE COUNT         1393 a          785 c          732 g         1369 t
ORIGIN      
        1 ataattctat caaaagtggt cagccggtga cttacccggt taatgatttt atcaaaggct
       61 ggactgaagg tttaaaaatg atgaaagaag gtgacaaatg gaatttatat gtgccatctg
      121 aattaggtta tggacctggt ggtttccaag gttttatacc acctaacgct actttaattt
      181 tcgaagtaga attattaaaa gttactaaag ggggcgctgc acctaaaatg aataaataat
      241 tttgtgtaac cacaactgtt aaaaccacgc caaaagcgtg gttttgctgt ttaaaccaat
      301 actttttcag ttagtcttat ggcggaaagt tgacaaattt gtcggtgctg aaattttaat
      361 atacgcttaa cattaactta atttacagga taattgatag tttacatatg aaaaaacgtt
      421 tccttgggct agtattaata gctttaaccc tctcctcctt tggtcagaca attgctatta
      481 ctgaaatcaa ctataacagc gacccttata acaaatccaa cgactgggtt gagttacata
      541 attatggtgg ctcagctgtc gatatatcta actggaaatt gaaagatgaa acaccattta
      601 attcatttac tataccggca ggtactgtat tggatgcaaa tgaatatgtt gttttagtag
      661 aaaatattga cacgttttta atggtgcatg gcggtgttac aaattacatt ggcgaatttg
      721 catttgggtt tgacaatact ttcggcactg taaaaattga aaatagttca ggcactgtaa
      781 ttaaagctgt gccttatatc gattcaattc catggccaaa aggttgtgat ggttatggcc
      841 caacactaca aattataaat gaagaagcaa gtgaaagtaa tcctgcaaac tggcgttcag
      901 gttgtgtttt aggcacaccg ggagaagctt acgcagattg caattatgat attgttgtaa
      961 gtgaaatcaa ctataattca ttattagcgt ataatcccgg cgactggatt gaaattttaa
     1021 atcgaggtaa tgcagataag gatatcagcg gctggatttt acgcgatggt aaaactgata
     1081 atatttttgt aatccctgcg ggtaatgtat taagtgcagg tgaacgatta gttatagcag
     1141 atagtttaga atcttttaca gttaaattcc ctacagtagg aaatgttatt ggggaaccac
     1201 cttttagttt ctcaaatggt ggagatgcag tgcgcttata cactccttcc ggtaaaattc
     1261 aatacagcgt tcgctataag gattcagcgc catggccttt agaccccgac ggcacaggat
     1321 ttacactcga actagttgat gaaccaggca atccaaacga tggtgttgca tgggttgcag
     1381 gctgtttgta tggttcaccc ggtaatccat ttattgtacc ttgtccgaat gcggtaaata
     1441 atgtaatacc taatgaatta acaattggac caaatccatt tactgattat ttgaccatca
     1501 ttatagatgc aacagtgcaa gcgcaatcgg taacgataat gaatacttta ggagaacaaa
     1561 ttattacatt gccaacttac aataatgctg ttacttggaa tagtaccgat ttgcaaggta
     1621 atacagtaag tgacggaatt tattttgtgg tagtaaccga taataatggc aatactataa
     1681 cggcaaaagt tttgaaagct aaataaaaaa aagcagtaat aaaaaagcga atatcaacgg
     1741 gacctacctg tttatattcg ctttttttat tttaaaaact gttgttaaac cttgggttta
     1801 ccacattatt gtaaatactt ccaaatgtat aagtcaaccc aaaataagtg taaaaactaa
     1861 aatcagtttg acgttcggtg agttgcaata atatttcttc atcggtcaac tcccctttcg
     1921 gtaaattcaa ttggtcgtta ataagattgt aattaaaaaa tgtattaaac gataaacctt
     1981 ctacaatact ccagttaata gaagcagaca acccagcact atttttacta aagtcatgaa
     2041 aataattggt gccatttaaa ccaaacgaaa cagagcccca tttttgaata agcgatagtt
     2101 caacttccaa tcgctctcgt aacaataatt cttccttttt gttaaacaca gttgtatcag
     2161 tataaatagc gtaatcagga ccggctttat acgtaattgt gagtaattta gtatttgatt
     2221 tggaatacgg gaaaacatta tactctattg caggatataa actgaaatcc gcatcatagt
     2281 tactataagt gcttgaaaaa taatcaacat aaccacctac cgaccagtgt tcacttatac
     2341 tgttagcgat taatccggta gcataagtgc cgttattaaa actgcggtat ttattgccat
     2401 caccaaaatc aaaattcgaa gtattataat atgcaccaag tgaaaaggat aatttccatt
     2461 cttcagttat tttatcaata cttaaatctc cgcttaaatt gtaatactct gaatttgaag
     2521 ttaaactcaa gttgccatat ccgcctacat taaataccca gccattccat ttatcatcca
     2581 cttgtttttc atttgtagtt gttgaaatat tagaagcaat ggacaccggg aaattggcac
     2641 tttttttagc aatgaagggc attaatccca ttttcatata actaacaata cctgaacgtc
     2701 gctcatcgtc agtattgttc gtgttatcaa aaaaatgcag ggtatcattt ttaccggcat
     2761 aactgagctg ccctaaaaat aatatggcat aatctgtccc cccacttccc gtggtttgtc
     2821 ctgtaattaa taaatgcaca tctgccaaaa accggtcccg aacataattt acatacggaa
     2881 tttctgtttt aatatactcg tgatattgat aaaccccatc gagataaaca tttaaaaaat
     2941 tgtcattcac agaatcacta gcgctggtag actcaatttg ttgggcactc aaagcaactg
     3001 tactacaaaa tagtacaata aggcaaacgg gaaatttcat aggtaatggt tataggtaaa
     3061 taaaaaaaaa tcaaatcaaa ttcatcatcc acgactcggc taaaggaatt ttgaatccct
     3121 tatcaaaatc tcttttttgt tgcacaagag ggtcaaatac cattttggat gcatttattt
     3181 ctccggattt aaatttcgtg ctaagttcat ctaaagaaat agtctggatt attccattta
     3241 tttccatatt tacaatagtt ctgttaaata aatcaattgt ataatcctgc tccagttgtt
     3301 taatgaaatg cactgaagta tcaatagaac atccgctggc catagtatgg ctttcatcta
     3361 ccattaatac aatgaatctg tcgtacatta cattccccga cgccaataac tgtttgttat
     3421 gagcagtcca ttgaacggta aaatgtttca gtttattatt taactcaact atttccgatt
     3481 cagtgaatgg acgcgaggat tgataaaccc acactttaga ttgaggagaa aaattttgca
     3541 tagtttatta tttataggta agctgcatga agagctaaca tcacattaaa tgagtataac
     3601 aattacgcca taagttgatt ggccattgca attaattcag caacatcaaa tactttgact
     3661 tcatcttctt tgtttttaat tttcacacca tcggtcatca tggtattaca aaacggacag
     3721 gcactagcta taatatcagg ttttacttcc agtgcttgtt cggtgcgttc aatattaatt
     3781 tctttgttac caggttcagc ttctttccac atttgcgctc cacctgcgcc acaacacaat
     3841 ccttttgatt tacaactttt catttcagta agcgctccgt ctaatttttc caaaacacta
     3901 cgtggtgctt cataaatctg gtttgctctt cccagataac aagagtcgtg aaaagtaatg
     3961 cgtttacctt taaatgcacc accttgcacg gtcaattttc cttcatcaat cagcgattgt
     4021 aaaaaagaag tatgatgcat tacttcgtaa ttaccaccta attccggata ttcattttta
     4081 agaatattaa aacaatgcgg acatgtggtg acaatttttt taatgccata accattcatc
     4141 acagcaatat tattcgcagc caacatttga aataaaaact cgttgcccgc tcttctggcc
     4201 ggatcgcctg tgcaaccttc ctctgttcca agaacggcga attttatttg tatactattt
     4261 aatatgcgga caaacgccc
//
LOCUS       DAORKW010000308         4273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1001_length_4273_cov_4.224751, whole genome shotgun sequence.
ACCESSION   DAORKW010000308 DAORKW010000000
VERSION     DAORKW010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1001_length_4273_cov_4.224751"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..477
                     /locus_tag="PLB46_11415"
     CDS             7..477
                     /locus_tag="PLB46_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRF77174.1"
                     /translation="MKLATGQQAPDFLMYDTDKQAVRLSEQQGKNVLLLFFPFAFTST
                     CTAELCATRDDIAFYQNVNAQVYGISVDAPYSLKVFKEEQQLNFPLLSDFNKTVSETY
                     GCIYDRWGMELKGVSKRSAFVIDKNGCIQYAEILENAGEIPNFEAIKKVLESLS"
     gene            516..926
                     /locus_tag="PLB46_11420"
     CDS             516..926
                     /locus_tag="PLB46_11420"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRF77175.1"
                     /translation="MFNVRVYGLLIHNNQILIVKEPIKNNQVIKFPGGGLEFGEGTID
                     GLKREFKEELGVTITDVTHFYTTDFFVPSFLNPEHQVISIYYLVKANPDELNFNNAEG
                     LTFSWIPLSTLSPSLFPLPIDKHVVQLLTTSQAY"
     gene            complement(942..1292)
                     /locus_tag="PLB46_11425"
     CDS             complement(942..1292)
                     /locus_tag="PLB46_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRF77176.1"
                     /translation="MKYIFIGTTIILAAINFYSCTTKTANAKTDQIKVWGNCGMCEKT
                     IEGALTLDGIVSAEWDKKTDILTVLYDSLKMNNPTIQMAIANAGYDTENMYANDSVYS
                     QLPKCCLYERKPKE"
     gene            complement(1312..2391)
                     /locus_tag="PLB46_11430"
     CDS             complement(1312..2391)
                     /locus_tag="PLB46_11430"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRF77177.1"
                     /translation="MRKIYWVILTIIPLFILLTITSCKEDEIVEPEVTYDPTPYAFDL
                     PDWVEVNIGEISAPIDNPTTIAGVELGRALFYDERLSNDMTMSCGTCHKQANAFDDPR
                     PFSQGTNGVFGNRNAMAIINLAWDGSFFWDGRQQSLEAQAHDPVTNPIEMANTWVEVE
                     NRLRDDATYQDMFKRAFNTTVIDSILITKAIAQFERTLVSFDSPFDAYYYGGDATALT
                     ADQISGMNLFFGDAKCFQCHSDPLFTDNAFRNNGLDLMPLDSGRAKFTGLATDYGKFK
                     VTTLRNIANTAPYMHDSRFATLEQVVEHYSSGVIQESPNLDEHMVDFGSGLNLTTTEK
                     MQLVAFLQSLSDNDFLTNQLFADPE"
     gene            complement(2401..3177)
                     /locus_tag="PLB46_11435"
     CDS             complement(2401..3177)
                     /locus_tag="PLB46_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77178.1"
                     /translation="MKNLITSFLLLTICSTAFSQATVAMDWTKTDCVAGNTYNLFDVL
                     NSDKVVVQEYVMMDCAPCITAGNVLMDLLEDYAATNPGEIVAYQTAFDNFTDCSTLSE
                     WATDNGFTATTLFTMGDDEINYYGAMGMPTILIFGGGENHTVYYYHLGSISSAVSKEE
                     FTTALELALEETGYTVGIENTLADKNIISPNPAVDVINISTTDNVLSYTIIDITGKTM
                     MTTTGLGNTQINVSGLIPGIYTVIAKAENGTVNSTFFVKQ"
     gene            complement(3236..3619)
                     /locus_tag="PLB46_11440"
     CDS             complement(3236..3619)
                     /locus_tag="PLB46_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77179.1"
                     /translation="MKKVLLCIFAFIYLWVSGGVVFSMHYCGDVIADVQPAITGTADC
                     CSSMPEDAGCCHDKLISIKINEAHYSTPTLQVINPEKTPSDITTVPLFNLQHQELEVN
                     STFFANNSPPITDNQRYIFISVFII"
     gene            3801..>4273
                     /locus_tag="PLB46_11445"
     CDS             3801..>4273
                     /locus_tag="PLB46_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRF77180.1"
                     /translation="MVFLDFEKPIADLYEELEKLKQMGEKQKIDITVPAAELQERIAN
                     LKRDIYENLNGWQKVQLSRHPDRPYSLFYIENMAENFVELHGDRNVKDDKAIIGGFAT
                     LEGQTVMFIGHQKGINTKMRQYRNFGMANPEGYRKALRLMKLAEKFNIPVVTLIDT"
BASE COUNT         1366 a          797 c          744 g         1366 t
ORIGIN      
        1 gttaacatga aattagcaac aggacaacaa gcacccgatt ttttaatgta cgataccgat
       61 aaacaagcgg taagattatc tgaacaacaa ggcaaaaatg tattactctt atttttccct
      121 tttgccttta cttccacctg cacggccgaa ttatgtgcca cgagagatga tatagcattt
      181 tatcaaaatg taaatgcaca ggtatatggt atcagcgttg atgcccctta ttcattaaaa
      241 gtatttaaag aagaacaaca actcaatttc cctttgttga gtgattttaa taaaaccgtt
      301 tcagaaactt atggttgtat ttatgatcgt tgggggatgg aattaaaagg tgtatcaaaa
      361 cgcagtgctt ttgtaatcga caaaaatggt tgtattcaat atgcagaaat tttagaaaat
      421 gcaggagaaa ttcccaattt tgaagcgata aaaaaagtgc tagaaagttt atcttaattt
      481 cacgcccggg taattccggg ctttatttat tttatatgtt taatgttcgt gtttacggtt
      541 tattgataca caataatcaa atactgattg taaaagaacc gattaaaaat aatcaggtaa
      601 ttaaatttcc cggtgggggt ttagaatttg gcgaaggcac catcgatgga ttaaaacgcg
      661 aattcaaaga agaattaggt gttacaataa ctgatgtaac ccatttttac accaccgatt
      721 tttttgtacc aagttttctc aatcctgaac atcaggtaat cagtatttat taccttgtta
      781 aagccaaccc tgatgaatta aattttaata atgcagaagg attaactttc agctggatcc
      841 cgctttcaac cctttctcca tcactatttc ctttgcccat cgacaaacat gtagtgcaat
      901 tgctaacaac atcacaagcc tattaacctc aagtaataat tttattcttt aggttttctt
      961 tcgtacaaac aacatttggg caactggcta taaacgctgt cgtttgcata catgttttca
     1021 gtatcatatc cggcattggc aatggccatt tgaatggttg gattattcat ttttaagcta
     1081 tcatacaata ctgtgagtat atcggtcttt ttatcccatt ctgcagagac aattccatct
     1141 aatgttaaag cgccttcaat tgttttttca cacataccgc aattacccca aactttaatt
     1201 tggtctgttt tcgcatttgc agttttagtt gtgcagctat agaaatttat agctgctaaa
     1261 attatcgtcg taccaataaa tatatatttc attgacgtgt attttaatga attattccgg
     1321 gtcagcaaac agttggttag ttaaaaaatc attatcactc aacgattgta aaaatgcgac
     1381 aagttgcatc ttttcagttg tcgttaaatt taatccgctg ccaaaatcta ccatatgttc
     1441 atctaagttt ggcgactcct gaattacacc actgctgtaa tgttcaacca cttgttcgag
     1501 tgttgcaaat cgcgaatcat gcatgtaagg tgccgtattt gctatattgc gtaaagtagt
     1561 cactttaaat ttgccataat cggttgcaag tcctgtaaac tttgctcttc cactatctaa
     1621 tggcattaaa tcaagtccgt tatttctaaa tgcattatcc gtaaacaatg ggtcgctatg
     1681 acattgaaaa cattttgcat caccaaaaaa taaattcatc ccactaattt ggtctgcagt
     1741 taatgcagtt gcatctccac cgtaataata ggcatcaaat ggtgaatcaa atgatactaa
     1801 cgtacgttca aattgagcaa ttgctttggt aattaaaata ctatcaatta cagtagtatt
     1861 aaaagcgcgt ttaaacatat cctgataggt ggcatcgtcg cgtaaacgat tttcaacttc
     1921 cacccaggta tttgccattt caataggatt agtaacaggg tcatgcgcct gtgcttccag
     1981 cgattgttgt cttccatccc aaaaaaatga accatcccat gctaagttta ttattgccat
     2041 tgcattgcgg ttgccaaaca ctccattggt gccctgacta aaaggtcttg ggtcgtcgaa
     2101 ggcattggct tgtttatgac aggttccgca actcattgtc atatcgttgg ataatcgttc
     2161 atcgtagaat aatgctctgc ctaactcaac acctgcaatt gtagtaggat tatctatagg
     2221 tgcactaatt tcccctatgt ttacctcaac ccaatccggt aaatcaaaag cataaggtgt
     2281 tgggtcatag gtaacttcag gctcaactat ttcatcttct ttacatgaag taattgtcag
     2341 caaaataaat agcggtatta tggttaaaat aacccagtat atttttcgca taaacaggat
     2401 ttattgtttt acaaaaaatg tactgtttac tgttccgttt tcagctttcg caataacagt
     2461 ataaatacct ggaataagtc cgctgacatt tatttgagta tttcccaaac cggtagttgt
     2521 catcattgtt tttcccgtga tgtcaattat cgtataggat aaaacattgt cagttgttga
     2581 aatattaatt acatcaacag cagggtttgg agaaatgata tttttatcag ccaaggtatt
     2641 ttcaattcca actgtataac ccgtttcttc taaagctaat tctaatgcag tggtaaattc
     2701 ttccttacta acagcagatg atatggaacc taaatgataa taataaacag tatgattttc
     2761 accaccacca aaaattaaaa tggtaggcat tcccatggcg ccgtaatagt taatttcgtc
     2821 atcgcccatc gtaaacagcg tagtagccgt aaacccatta tctgtagccc attcgcttaa
     2881 tgtagaacaa tccgtaaaat tatcgaaagc tgtttgataa gcaacaattt cacccggatt
     2941 tgtagcggca taatcttcaa gtaaatccat taaaacatta cctgcagtaa tacaaggtgc
     3001 gcaatccatc atcacatatt cctgtacaac tactttgtcg gaattaagca catcgaataa
     3061 attatacgta ttacctgcaa cacaatctgt ttttgtccaa tccatagcaa ctgttgcctg
     3121 actgaacgct gtagaacaaa ttgtcaataa aagaaagctg gttattaaat ttttcatcgt
     3181 taaatatttt tcaagttaat taattaaaca acaggtttaa ataaagacat gaaatttaaa
     3241 tgatgaaaac agaaataaaa atataccgtt gattatcggt tattggaggt gaattatttg
     3301 caaaaaaagt actgttaact tcaagttcct gatgttgcaa attaaacaaa ggcactgtag
     3361 taatatccga tggtgttttt tccggattaa tcacctgtaa tgttggtgtg ctataatgcg
     3421 cttcattgat tttaatggat atcagtttgt cgtgacaaca tcctgcatct tcaggcatgg
     3481 aagaacagca atcggcagtc ccggtaatgg caggctgaac atcagctatt acatctccgc
     3541 agtagtgcat agaaaaaacc actccaccgg aaacccagag ataaataaaa gcgaaaatgc
     3601 aaagcagcac ctttttcatt gatggtataa agttaccatt tttttagctt tggaaacggt
     3661 ttatttcgac catttatgca cagtaaaaaa ggaagattac cttacctaaa agatgttttc
     3721 aggggtaaaa aactgcattt taccccattt tgggtgtatt tgagtttatt tttaccttaa
     3781 ttattcgata aatttgcgac atggtatttc ttgacttcga aaaacccatt gctgatctgt
     3841 atgaagaatt ggagaagctg aagcagatgg gtgaaaagca aaagattgat attactgttc
     3901 ctgctgctga attgcaggag cgtatagcta acctgaaacg cgacatatat gaaaacctga
     3961 atggttggca gaaagtccag ctcagcaggc atcctgacag gccttattcc ctgttttata
     4021 tcgaaaacat ggctgaaaat tttgtcgaat tacatggcga ccgcaatgta aaagacgaca
     4081 aggccataat tggcggtttt gccacattgg aaggccaaac agtaatgttt atcggtcacc
     4141 aaaaaggtat caacacaaaa atgcgtcaat atcgcaattt tggtatggcc aaccctgaag
     4201 gttatcgcaa agccttgagg ctcatgaagt tagctgaaaa atttaatatt cctgtcgtta
     4261 cattaattga tac
//
LOCUS       DAORKW010000309         4271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1002_length_4271_cov_4.464421, whole genome shotgun sequence.
ACCESSION   DAORKW010000309 DAORKW010000000
VERSION     DAORKW010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1002_length_4271_cov_4.464421"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PLB46_11450"
     CDS             complement(<1..279)
                     /locus_tag="PLB46_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77181.1"
                     /translation="MDQELADMALQNLNQIAGIQAKWNNKGPLDGILEIKVNGQKHTF
                     IVEVKREIRNYQLPQVEDDFRKNEQFMLLANRLFPKIKEELRQQEIPSL"
     gene            complement(419..1048)
                     /locus_tag="PLB46_11455"
     CDS             complement(419..1048)
                     /locus_tag="PLB46_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77182.1"
                     /translation="MHITIISIVTLITILAAIRIKGRLAKVWVIIIGSIIVFITAALL
                     FINYFFSAFAPPQIKITSDYITTNHDFVNGIAIEKIRVDSIGEDFYPVKYTMTYRTTC
                     SIKHPIGKPPSPPDKIYFYEKGAYVWWEEHKTRTFIHEGLGRTQIKVEENNELNRTHF
                     TYAICPLQFEREQWYFFTIGDPQITGIFFYIDKNGNEKQYYLESGVSPI"
     gene            complement(1166..1657)
                     /locus_tag="PLB46_11460"
     CDS             complement(1166..1657)
                     /locus_tag="PLB46_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77183.1"
                     /translation="MKITFPIKVNLFRLTFLVLAISGWILYDAQLEDYTLLITIILVF
                     GSLFTVASAEIFGNTLLVRKNYCWGIFPLTKSLPLDKIQSIHSKDYSIQSYGDLDSGS
                     GNIFVNIFFEFTKPKVKWVVSKIVYLDKKKTKTIEAKVTDVIFRQIENSILDKNQNDQ
                     TAQ"
     gene            complement(1854..2186)
                     /locus_tag="PLB46_11465"
     CDS             complement(1854..2186)
                     /locus_tag="PLB46_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77184.1"
                     /translation="MREKGTQALEETIDQEVDFSGVKTLIAFQKNQFNIFQSTSEPFK
                     LEYEGEWEINNAAIKALIFETFVNQGIKADSSATSVETIDGLDFQTYSFTIYGPNDEV
                     ILNQILYS"
     gene            complement(2805..>4271)
                     /locus_tag="PLB46_11470"
     CDS             complement(2805..>4271)
                     /locus_tag="PLB46_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77185.1"
                     /translation="TKMTTNLEERIFDSQWYKNNIQTPIRSNAIELKLAKNELGQFIK
                     LKELRVPEPTLKKEEVNSIWEYYSVLFPERICKFEEIKYWQIIMWNDFPYLDCKRIVA
                     AAKSFENLSDLSSKINKQPNETVLWLNNLYKLIYTLSGNDLQFRENELIPNLDGNFRK
                     LQELNIDGVGQPILLQILQLFGTNWKDKILYPNSCVDTELRFKTFNFSNVKVDIEKAL
                     NNLKDKSTTINEATIMLVQWLEKNENLAVEHFKELYKNRADLFVGAIPDKDSLYAIFR
                     DGVDLSKVKKIIEFVSLHPDIDFDNVQDNMNQYKLLPDTTNNIYKEALELLVAELRKK
                     NVSTIHELLDVLERIKDGELIKRADNGNSEIQILPEERAKLFQSLLAEAIQTTIPHLK
                     SHGYIFSDELDQNSPSVFRVIRNNETIKIIIRPAHGGKYKLFYDGEFDALEAKSTELW
                     LSDGRNVFQETLGLLVKRMLAEGTRFIPTSGFLPGNSI"
BASE COUNT         1278 a          743 c          708 g         1542 t
ORIGIN      
        1 caaagagggg atttcctgtt gacggagttc ttctttgatt tttggaaaaa ggcgattggc
       61 caaaagcatg aattgctcat ttttgcggaa atcatcttcc acttgtggta gttgatagtt
      121 tctaatttca cgtttgactt ccacgataaa ggtgtgtttc tggccattta cctttatttc
      181 caatatccca tccagtggac ctttgttatt ccatttagcc tgaatcccag ctatttgatt
      241 caggttttgt agggccatat ctgcgagttc ctggtccatt ttattgcttt aagttcacaa
      301 atatacattg ttcacgtgac gtgaacaagt gttaaaagtg aacaattggt aagccaaacc
      361 ccaacttgtt tggtatttga ggctaaattc caactcgggc aaccgattca gcattttttt
      421 agataggtga aacaccactt tccaaataat attgtttttc gttgccattt ttatcaatgt
      481 aaaaaaatat gccggttatt tgcgggtctc caattgtaaa gaaataccac tgttctcgtt
      541 caaattgtaa tggacatatt gcataagtaa aatgcgtgcg atttaattca ttattttctt
      601 caacctttat ttgggttcta cccaaacctt catgtataaa ggtccgtgtt ttatgttctt
      661 cccaccagac atatgctcct ttttcgtaaa agtaaatttt atcaggaggg ctgggtggtt
      721 ttccaattgg atgtttgata ctgcaggtgg tacgatatgt catcgtgtat ttaacaggat
      781 aaaaatcttc cccaatagag tcaaccctta ttttctcaat agcaatgcca tttacaaaat
      841 cgtgatttgt ggttatataa tcagatgtaa tttttatttg aggcggtgca aacgcactaa
      901 aaaaatagtt tataaatagg agggccgcgg ttatgaagac aattattgaa ccaataatta
      961 ttacccatac cttagccaat ctgcctttga tacggattgc ggctaaaata gtaattagtg
     1021 taactattga tattatggtt atatgcattg gagcaatatt ttatttaagg tgcattcaac
     1081 tctttagaag ttatatcatt ttaattggat tgcatacatt ccaaaaactt tcatttcatc
     1141 attcaataaa tatacaaaaa ttaagttatt gagccgtttg atcattttgg tttttatcca
     1201 gaatactatt ttcaatttgc ctaaaaataa catctgtaac cttggcttca atggttttgg
     1261 ttttcttttt atcaaggtag acaattttgg ataccaccca cttaactttt ggttttgtaa
     1321 actcaaaaaa tatattgaca aaaatattac cggaaccgga atccaaatcg ccatacgatt
     1381 gaatggaata atcttttgaa tgaatgcttt gtattttatc taatggtaaa gacttcgtta
     1441 atggaaatat cccccaacaa tagttttttc taacaagcag tgtgttgcca aatatttcag
     1501 cagatgcgac agtaaataga ctcccaaaaa ccaatatgat ggttatcagc aaagtataat
     1561 cctcaagctg tgcatcatat aaaatccaac ccgaaattgc taacactaaa aatgttaagc
     1621 ggaacaagtt tacttttatg gggaaggtta ttttcataat ttacaacctt tttcaggatg
     1681 catcatgacg cgtattttat gacattgata cacttactga aaaccaaaat tacatttatc
     1741 tcacctcatt ttcatttttt aaacttagaa ctcctaaatg cctgtaactg tgttgcttta
     1801 tctttttcgt tgttgcaatt gatatttgct gcaaagtcaa atccattgat taatcagcta
     1861 tataaaattt ggttcaaaat gacctcatca ttcggaccat aaatggtgaa gctatatgtc
     1921 tggaaatcta aaccgtctat agtttctaca gaggttgcag aggaatccgc ttttatgccc
     1981 tgattaacaa aagtttcaaa aattaatgct ttaattgctg cattatttat ttcccattct
     2041 ccttcatact ccaatttaaa cggctcggaa gttgattgaa aaatattaaa ttgatttttc
     2101 tgaaaagcaa tgagcgtttt cactccactg aaatcaactt cttggtcgat tgtctcctcc
     2161 aatgcctgag tacctttttc tctcatatct tgtgattttt ccctatcgac cacttcccaa
     2221 ccatccggta ttgctattgt ccaaccaatt tcctcacttg tataagtaat gccctcaact
     2281 ttaccttcgt caatattttt gttagggtct gagggtttgc aagagattat tgcaagggtt
     2341 gagatgaata ttaatagtgt tttttcatgt agtttgtttg aatgttgatt tgattatgaa
     2401 gttgtttgtg gctttgtgct agcttggata atagttcaaa tgtattaatt ggattgggat
     2461 tttgcaagtg gttgcgaagg tcaatgtgac ggcaaattca aacactttgc taaattttgg
     2521 ggtagcagta ttttacttaa ctaaaactat cgaagaaata attatgtctg agtagggttc
     2581 ttgcatcatg aaaaaataaa aaccgcaaat cgctttgagc tttggaaatt ttattgtgta
     2641 attgtaataa gtaaagactc aaatatcgca gggttattct ggaaaaaaaa ttataattgc
     2701 aggctggtcc tgttcgacct aacactttcc tcggaataac gaagcatttt gaaattgaat
     2761 gttggattca gatttctcat gaatgtaaaa tagatatttc gtaattaaat tgaatttcca
     2821 ggtaagaagc cacttgttgg aatgaatcta gttccttctg ctaacatcct tttgaccaaa
     2881 agccccaagg tctcttgaaa tacatttcgt ccatctgaaa gccaaagctc cgtagatttg
     2941 gcctccaagg catcaaactc tccgtcgtag aaaagtttat acttaccacc atgtgctggt
     3001 cttattatta ttttgatagt ttcattattt cttattacac ggaataccga agggctattt
     3061 tgatccaact catctgaaaa tatatacccg tgagatttca gatgtggtat cgtggtttgg
     3121 attgcctctg ccaacagaga ctgaaacagt ttcgcacgct cctccggtag tatttgaatc
     3181 tccgaatttc cattatcagc tcgcttaatc aactcgccat ctttaatcct ctcaagtaca
     3241 tccaaaagtt catgaattgt ggatacattt tttttacgta gctctgcaac aagtaattct
     3301 aaggcttctt tgtaaatatt attagtagta tctggtaata atttatattg attcatatta
     3361 tcttgaacat tatcgaagtc aatatctgga tgcagagata caaattcaat aattttctta
     3421 actttactta aatcaacacc gtctctaaat attgcatata agctatcctt atcgggtatt
     3481 gctcctacaa ataaatctgc ccgattttta taaagctctt tgaaatgttc aacagcgaga
     3541 ttctcatttt tttcaagcca ttgtacaagc ataattgtgg cttcattgat tgttgtagat
     3601 ttatctttaa gattattcag agccttttcg atatctacct taacatttga aaagttaaaa
     3661 gttttaaatc ttagttccgt gtccacgcaa gagtttgggt acagtatttt atccttccaa
     3721 ttagttccaa atagctgcaa aatttgaagc agaatcggtt gcccaacacc atctatattt
     3781 aattcttgca attttctaaa atttccatct agattcggaa ttagctcatt ttcacgaaat
     3841 tgtaagtcgt taccagataa ggtgtaaata agtttataaa ggttattgag ccataggaca
     3901 gtttcattag gttgtttatt aattttagaa gatagatcag ataggttttc gaacgattta
     3961 gcagccgcaa cgattcgttt gcaatctaga tatggaaaat cgttccacat aattatttgc
     4021 caatatttaa tttcttcaaa tttgcaaatt ctttcgggaa ataaaacact ataatattcc
     4081 caaatagaat ttacttcctc ttttttgagg gtaggttcgg ggactcttaa ttcctttagt
     4141 ttgataaatt ggccgagttc gtttttagcc aacttaagtt caattgcgtt tgatctaatt
     4201 ggagtttgaa tgttattttt ataccattgg ctgtcaaaaa ttctttcttc taaatttgta
     4261 gtcatttttg t
//
LOCUS       DAORKW010000310         4256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1005_length_4256_cov_4.007855, whole genome shotgun sequence.
ACCESSION   DAORKW010000310 DAORKW010000000
VERSION     DAORKW010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1005_length_4256_cov_4.007855"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLB46_11475"
     CDS             <1..433
                     /locus_tag="PLB46_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRF77186.1"
                     /translation="KFHELDIPVELIPEIKKGKDVLDEKTGERILNKDITLDPLRARA
                     YAYCCDTAFNPEMAAQIKGVDLLYHDCTFDAAGEQRAADTFHSTTIQAAQMASLAAVN
                     KLMIGHFSSKYDDLRPLLEEAKTVFENSILAIEGKVVVVER"
     gene            complement(487..636)
                     /locus_tag="PLB46_11480"
     CDS             complement(487..636)
                     /locus_tag="PLB46_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77187.1"
                     /translation="MSKKRADKKQATQQVKEESVASTAFNIVIGIIIVGIMALIIVWM
                     MSALL"
     gene            complement(639..1994)
                     /locus_tag="PLB46_11485"
     CDS             complement(639..1994)
                     /locus_tag="PLB46_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRF77188.1"
                     /translation="MANILLIDDEKSIRKTLKEILEYEGYVVDEAGDGAEGLRMIQEG
                     DYDIALCDIKMPKMDGMEVLEKAQISNPDVPFIMISGHGTLETAVEAVKKGAFDYVAK
                     PPDLNRLLITIRNALDKSNLITETKVLKRKVTKTREIIGDSPAIQKIKETIERVAPTD
                     ARVLITGDNGTGKELVARWIHEKSNRANGPLIEVNCAAIPSELIESELFGHEKGAFTS
                     AIKQRIGKFEAANGGTLFLDEIGDMSQSAQAKVLRALQENKITRVGGDKEIPVDVRVV
                     AATNKDLIKEVENNTFRLDLYHRLSVILIHVPSLNDRTDDIPLLSERFVKEICEDYGV
                     PKKVITPKAMMEIMNINFTGNIRELRNVIERLVILSEQKITEEDVINYAQSPKSGGSP
                     KDLYERFEKFQDFKDHIEKEFILHRLNKNAWNVSKTAEELDIQRSHLYNKIEKYNLKR
                     D"
     gene            complement(2163..2963)
                     /locus_tag="PLB46_11490"
     CDS             complement(2163..2963)
                     /locus_tag="PLB46_11490"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HRF77189.1"
                     /translation="MKKSNLLLSFLLAFCSMSYAQNLVTYLPPDPKTAPPAQAPIDTI
                     ITVIDAAKNYKHIVLTQNNITIQDGYLVGNKKTGKWYNYAPTGVLINLAEYENDVKSG
                     IYIEFDKSGAVAIQETYKNGQLEGEQKKYGAGPNGRILKSSYTYKNGQYHGTCTEFTD
                     NGLIRSLVQYKEGKKDGDTKWFYTSGKLAMQQTYSNNVLEGPQKVYNQQGILTSDGQY
                     KAGLKNGNWTEYYDSGKMKSQGAYLDDKQTGKWSFFNEDGTISKTETF"
     gene            complement(2950..3189)
                     /locus_tag="PLB46_11495"
     CDS             complement(2950..3189)
                     /locus_tag="PLB46_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77190.1"
                     /translation="MEKMQIGNEILICPYCGQLTKIVWVHGHGQCMICKTNFDECCRG
                     ENISVDENNENVEENNQNNNTPEHQNEQQPEDEKK"
     gene            complement(3205..3586)
                     /gene="rnpB"
                     /locus_tag="PLB46_11500"
     ncRNA           complement(3205..3586)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLB46_11500"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3660..>4256)
                     /locus_tag="PLB46_11505"
     CDS             complement(3660..>4256)
                     /locus_tag="PLB46_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRF77191.1"
                     /translation="ATIKLHEFKTDQLNDAPMFVINCWLKTAEGGTQPSVSKEIKLKA
                     KQYFAKLDSPEFKKQDKFVLEVTKEIEPKVKKTPMVEVDKDDFFFEKKKIPAQHELIV
                     KAEMPDFIDLHAENLLPGYQHMDANDILYRQISHFKNFLEKAIKYNLHKIYVVHGIGK
                     GVLKAEIEKVLKKYPEVSSYNNDYHTRFGFGATEIFLD"
BASE COUNT         1154 a          834 c          769 g         1499 t
ORIGIN      
        1 aaagtttcac gagttggata ttcctgttga attaattccg gagataaaaa aaggaaaaga
       61 tgttttggat gaaaaaaccg gagagcggat tttaaataaa gacattactt tagatccgct
      121 gcgcgcgcgt gcttatgcat attgctgcga cacggcattt aatcctgaaa tggcagcaca
      181 aataaaaggt gttgatttat tataccacga ttgtacattt gatgcagccg gagaacaacg
      241 cgccgcagat acttttcatt ccacaaccat acaagcagca caaatggctt cacttgcggc
      301 agtaaataaa ttaatgatag gccatttttc ttctaagtat gatgacttaa ggcctttgct
      361 tgaagaagca aaaacggtgt ttgaaaatag tattcttgct attgaaggga aggttgttgt
      421 ggtggaacgg tgaaaaaaaa aatcccgcac ctttcagtgc aggattaaat acattcgaaa
      481 actattttac aacaacgcac tcatcatcca aacaataatt aacgccataa ttccgacaat
      541 aattattcct atcacgatat tgaatgcagt tgaagcgacg gactcttcct taacctgctg
      601 agtagcctgt tttttgtcag cgcgtttttt tgacatgatt agtcgcgttt taagttatat
      661 ttttcaattt tattatacag gtgactgcgt tgaatatcga gttcttcagc agttttggaa
      721 acattccagg catttttatt taaacgatgt aaaataaatt ctttttcaat atggtcttta
      781 aaatcctgga atttttcgaa acgttcgtat aaatctttcg gactgccacc gcttttaggt
      841 gattgcgcat aattaattac atcttcttcc gtaatttttt gctcacttaa aattaccagt
      901 cgctcaatta cgttgcgcag ctcacgaata tttccggtga agttaatatt cataatttcc
      961 atcatcgctt ttggtgtaat cactttttta ggcacaccat aatcttcaca aatttctttt
     1021 acgaaacgct ctgataataa agggatatca tcagttctgt cgttgagtga aggaacatga
     1081 atgaggatta cactcaagcg gtgatataaa tcgaggcgga aagtattatt ttccacttct
     1141 ttaattaaat ctttgttagt tgccgctaca acacgaacat caaccggaat ttctttatcg
     1201 ccaccaacgc gggtgatttt attttcctgt aatgcacgta atacttttgc ctgtgcactt
     1261 tggctcatgt cgccaatttc atctaaaaac aaggttccgc cattggcagc ttcgaatttt
     1321 ccgatacgtt gtttgatggc agatgtaaat gcaccttttt cgtgaccgaa taattcgctt
     1381 tcaattaatt cacttggaat tgccgcacag tttacttcga ttaaagggcc atttgcgcga
     1441 ttactttttt catgtatcca tcgcgcaacg agttctttac ctgttccgtt atcaccggta
     1501 attaaaacac gggcatcggt tggcgcaaca cgttcaatgg tttcttttat tttttgaata
     1561 gccggtgagt cgccaattat ttcgcgtgtt ttggtaacct tacgtttgag gacctttgtt
     1621 tcggtaatta aattcgattt atcgagggca ttgcgaatgg taatgagtaa acgatttaaa
     1681 tcaggtggtt ttgccacata gtcaaaagcc ccttttttta ctgcttcaac ggcagtttct
     1741 aaagtgccgt gaccggaaat catgataaat gggacatcag gattgctgat ttgtgctttt
     1801 tcgagcactt ccataccatc catttttggc attttgatat cacaaagggc gatatcataa
     1861 tcaccttcct gaatcatgcg gaggccttcg gcaccatcgc cggcctcgtc tactacataa
     1921 ccctcgtatt cgagaatctc cttaagagtt tttctgatgc ttttttcatc atcaattaat
     1981 aaaatgttcg ccatactgtt cgtttaagtg ttcactaaca gcctgcaaag cgcgtgaaac
     2041 ctgcatgctg ccgtattaag ttatttttca aaagtttaag taagtgtaca gtgttcgtac
     2101 acaaaactaa acgaaggtaa atataattta ttggacagag aaaataaaat ttgtagtttt
     2161 tttcagaaag tttctgtctt acttatggta ccatcttcat taaaaaacga ccatttgccg
     2221 gtttgtttat cgtccaaata agcaccttga gatttcattt taccggagtc gtagtattcc
     2281 gtccaattac cgttttttaa tcccgcttta tattgtccat cgctggttaa aattccctgt
     2341 tggttgtaaa ccttttgagg tccttctaat acattattgc tataggtttg ttgcatggct
     2401 aatttaccgc tggtataaaa ccatttcgta tcgccatctt ttttgccttc tttgtattgt
     2461 acgagggaac gaattaatcc attatcggtg aattcagtgc aagtgccatg atattgccca
     2521 tttttatagg tataactact ttttaaaatt ctgccattag ggcctgcacc gtattttttt
     2581 tgttcgcctt ctagttgtcc gtttttatag gtttcctgaa tagcaactgc tcctgattta
     2641 tcaaattcga tataaatacc tgatttaaca tcattctcat attctgctaa attaatgagc
     2701 acaccggttg gagcatagtt ataccatttg cctgtttttt tatttcctac taaataccca
     2761 tcctgaatgg taatattatt ttgggtaagc acaatatgtt tataattttt agctgcatca
     2821 attacagtga taattgtatc aatcggagcc tgagccggag gtgctgtttt agggtcgggc
     2881 ggcaggtacg taactaaatt ttgagcgtaa gacatgctgc aaaaagcaag caggaaagag
     2941 aggagtaaat tacttttttt catcttcagg ttgttgttca ttttggtgtt caggtgtgtt
     3001 attattttgg ttattttctt caacattttc gttgttttca tcaaccgaaa tattttctcc
     3061 cctgcaacat tcatcaaaat tggttttgca aatcatacat tgtccgtgtc catgaaccca
     3121 gacaatttta gtgagctgac cgcaataggg gcagattaat atttcgttgc caatttgcat
     3181 tttttccaaa aacgtcttta aataaattcc cataaactca taagccggat tctgtttgag
     3241 tttctatcat ttatctggtc ctgatgtcac catcaggttc ttgctgccta cccgccgaag
     3301 attaaaacga gcagcttttc ttttactccg gcatatttgg cattgtaacc cgcagggttt
     3361 acccgatact gatgttacca ccagcacccg tgagctttta cctcgcgttt tcaccatttc
     3421 cccgaagtga tgacacgaag tggcatctac ttccggggtt gttattttct gtggcactgt
     3481 tccgttatcc gccgaggcgg atacacacct gttaggtgtt gcggtgctct gtgttatccg
     3541 gactttcctc tctgcattgc tgccgagcga tagaacggtt tacaggaatt tatttaaaga
     3601 acgtttattt agtttgggac tttcatccca tcattttcaa tacgaaatta cagcaaattt
     3661 taatccaaaa aaatctctgt tgcaccgaat ccgaaacggg tgtgataatc gttattgtag
     3721 gaacttacct cgggatattt ttttaacact ttttctattt ccgcttttaa tacccctttg
     3781 ccaataccat gcactacata aattttatgc agattatatt taatagcttt ttctaaaaag
     3841 tttttgaagt gactaatttg tcggtataaa atatcattgg catccatgtg ctgatatccg
     3901 ggtaataaat tttcagcatg taagtcgata aaatccggca tttctgcttt aacaattaat
     3961 tcgtgttggg cagggatttt tttcttttca aaaaagaaat catctttatc cacttctacc
     4021 attggtgttt ttttaacctt aggttctatt tcctttgtca cttccaaaac aaatttatcc
     4081 tgctttttaa attcaggact atccagtttc gcgaaatatt gtttggcttt taatttaatt
     4141 tcttttgaca ccgatggttg agtgcctcct tctgctgttt ttaaccaaca attgataaca
     4201 aacataggag catcatttaa ctggtcggtt ttaaattcat gcaattttat tgttgc
//
LOCUS       DAORKW010000311         4256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1006_length_4256_cov_3.770769, whole genome shotgun sequence.
ACCESSION   DAORKW010000311 DAORKW010000000
VERSION     DAORKW010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1006_length_4256_cov_3.770769"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLB46_11510"
     CDS             complement(<1..340)
                     /locus_tag="PLB46_11510"
                     /inference="COORDINATES: protein motif:HMM:NF025617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HRF77192.1"
                     /translation="MKKSEIVIIIVAIFALIGIVILLRSINADDKFNWAESYRSDSDQ
                     PYGTLVITKLLDDYFPNDKLTLVAEDINKPLTEITGEHRTYMFIGSYPYYTDSTLENL
                     KQFVQRGNDVF"
     gene            complement(337..975)
                     /locus_tag="PLB46_11515"
     CDS             complement(337..975)
                     /locus_tag="PLB46_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HRF77193.1"
                     /translation="MLFISCILVHFVLFSQDEVPVYHPIEKSDWNKAVDGLSYNEKII
                     EEEEVEISQTTAPLNFGPLLQIIGFILIAALLIFVLFYFFGKGLFGNPKVESATQNII
                     TDLEERPMESDLERYLREALAAKNYRLAIRIYYLMMLKSLHEKSLITWKKDKTNMDYL
                     IELRNHPQYDALSKNTLIYEFVWYGDKSITDQHYNEASPLFIQLIQQLQSKA"
     gene            complement(984..2864)
                     /locus_tag="PLB46_11520"
     CDS             complement(984..2864)
                     /locus_tag="PLB46_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HRF77194.1"
                     /translation="MRETSFIEQNKQKWGEFEQKFEKESDPEKVSNLFIQVTDDLSYA
                     RTYYPNRSVKIYLNNLSQKVFQTIYKNKVRRRRKFQTFWTDELPQKMYDSRWQLLFTF
                     TLFMIAFFIGIFSSMHDPEFARFILGDSYVRMTEENIASGDPMKVYKEMNEFDMFFAI
                     TFNNLRVSFLTMIFGIFFGLGTAYFILYNGIMVGVFQYFFIERGLFAESFLTIWVHGA
                     LEISAIVIAGTAGITLGRGLLFPGTYTRAQSFRIHGLRAVQIFLGIAPIIVLAGINES
                     FLTRYTETPDIVRALLIILEFGFMLFYFVIYPARKAKKGFAVSRKSDEIPADKIPSIN
                     LKKIKTNGEIFADGFSALRLYSGPIMQVVLFTTIVYVGFYFWQIMSFSEVFHQIQILR
                     AAAIVESVRDVFMLLNYQEAPTPLAGYKLLALNVISLTIISSLAIYVLKLIKQKNQPF
                     LYTAFGKFLLTKGWFILLICLGLHSMLLINTTLIRWLFFAALPLVFMLFADISSKGKL
                     SGGIFRKANYYFKQFLNVNLLYMPLLVLSIMMACIAHSLICYFNVELIKWNIPFDPLI
                     YEAIQDISITFILMGVIFLQITMVAINIGLMYFSVKEIEDADDLQTRVQDLAHPKSMV
                     KG"
     gene            3019..3744
                     /locus_tag="PLB46_11525"
     CDS             3019..3744
                     /locus_tag="PLB46_11525"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRF77195.1"
                     /translation="MRTIDILTTQNVTINYELAEAKDRIFATIIDIIIQSIGLFFLSL
                     IFMSSGMMSEDYFLYFIVFPVLFFYYFGSELLMDGQTLGKRMLGIKVVKLTGTEPSIN
                     DYLMRWALRPIDIFFSLGSIGIMLVSSSDKAQRLGDIVANTTVIKTNPSQRIQLADIL
                     SIRNLSNYEPVYPQIRTFAEHEMLLLKEVMDKSLKYQNSSHEEALETACQLVKQRLNL
                     EKIPADRTSFIRTLIKDYVALSR"
     gene            3843..>4256
                     /locus_tag="PLB46_11530"
     CDS             3843..>4256
                     /locus_tag="PLB46_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRF77196.1"
                     /translation="MQEIRNIAIIAHVDHGKTTLVDKILHHCQLFSKEHSELILDNNE
                     LERERGITIVSKNVSVNYKGFKINIIDTPGHADFGGEVERVLNMADGALVLVDAFEGP
                     MPQTRYVLSKAIALGLKPCVVINKVDKENCKPDEVH"
BASE COUNT         1421 a          787 c          742 g         1306 t
ORIGIN      
        1 taaatacatc gttgccacgt tgtacaaatt gtttaagatt ttctaaggtg ctatcggtat
       61 aatagggata actgccaata aacatataag tgcgatgttc accggttatt tcagttaacg
      121 gtttattaat gtcttctgca actaaagtca atttatcatt tggaaaataa tcatccagta
      181 gtttggtgat taccaaagtg ccataaggtt gatcggagtc tgaacggtaa ctttctgccc
      241 aattgaattt gtcgtcagca ttaattgaac gtaataaaat tacaatacca attagggcaa
      301 aaatggcgac aattattatt acaatctccg attttttcat gccttagatt gtaattgttg
      361 aatgagttga ataaacaaag gacttgcttc attataatgt tggtcggtaa ttgatttatc
      421 gccataccaa acgaattcgt aaatcaatgt gtttttactt aaggcatcat attgtgggtg
      481 attgcgcaat tcaatgagat aatccatatt ggttttatcc tttttccatg taatcagcga
      541 tttttcatgt aacgatttta acatcattaa ataataaatc cgtattgcca gtctgtagtt
      601 tttcgcagct aaagcttccc gtaaatagcg ctcaaggtcg ctttccattg gtcgttcttc
      661 aaggtcggta ataatgtttt gagttgcaga ttcaaccttt ggattgccaa ataatccctt
      721 gccgaaaaaa taaaagagga caaaaattaa tagtgcggca attaaaataa atccaatgat
      781 ttgtaacagt gggccaaaat ttaagggagc tgtagtttga gatatttcaa cttcttcttc
      841 ttcaataatt ttttcattat aactcaaccc gtctacagcc ttattccaat ccgatttttc
      901 aatagggtga taaacaggaa cttcatcttg actgaataat acaaaatgta ccagtataca
      961 tgatatgaat aacaaaaagc gcatcaccct tttaccatcg attttgggtg agctaaatcc
     1021 tgaacacgcg tttgtaaatc atcagcatct tcaatttctt ttacactgaa atacatcaat
     1081 ccaatgttaa tcgctaccat ggtaatttgt aagaaaataa cacccattaa aatgaaggtg
     1141 atactgatat cctgaatagc ttcataaata agggggtcaa aaggaatatt ccacttgatt
     1201 aattccacat tgaaataaca aattaatgaa tgcgcgatac aagccatcat gatacttaaa
     1261 accaataatg gcatatataa taaattgaca tttaaaaatt gtttaaaata ataattggct
     1321 tttctgaaaa tgccaccact tagtttgcct ttggaagaaa tgtcagcaaa taacataaat
     1381 accaatggta aggcagcaaa aaatagccaa cgtatcaatg tagtattgat aagcaacatg
     1441 ctgtgtaacc ccaaacaaat taataatata aaccagcctt tggtcaataa aaatttgccg
     1501 aatgctgtat ataaaaaagg ttgatttttt tgtttgatta atttcaacac ataaatggcc
     1561 agactgctga taatggttaa acttattacg tttagcgcta ataatttata tccggctaat
     1621 ggtgttggcg cttcctgata gttcaataac ataaaaacat ctctgacaga ttcaactata
     1681 gcagcagctc ttaaaatttg tatttgatga aatacttctg aaaatgacat aatctgccag
     1741 aagtaaaaac ctacatatac tatggtggta aatagcacta cctgcataat tggaccgctg
     1801 tacaagcgaa gcgcactaaa tccatctgca aatatttccc cgttcgtttt tatttttttt
     1861 aagttgatgg atggaatttt gtcggcaggt atttcatctg atttgcgact cacagcaaat
     1921 cctttttttg cttttctggc cggataaata acgaaataaa ataacataaa tccgaactct
     1981 aaaattatca acagcgctcg tacgatatcc ggagtttcag tatacctggt taaaaagctt
     2041 tcattaatcc cggctaaaac aattattggg gcaatgccta aaaatatttg aacagcccgc
     2101 aagccatgaa tgcgaaacga ctgcgccctg gtataagtgc ctggaaataa taaacctcgt
     2161 cctaaagtga tacctgcagt tccagcaatt acgatggcag aaatttctaa agcaccgtgc
     2221 acccaaatag ttaaaaatga ttcagcaaat aaaccgcgtt caataaaaaa gtattgaaaa
     2281 acgccgacca ttataccatt gtataaaata aaataagcag ttcctaaacc gaaaaaaata
     2341 ccaaatatca tcgttagaaa tgatacacgt aaattgttaa aggtgatggc aaaaaacata
     2401 tcaaactcat tcatctcctt atacactttc atcgggtcac cggaagcaat gttttcttcc
     2461 gtcatacgca cataactgtc ccccaaaata aatcgcgcaa attcaggatc atgcatactt
     2521 gaaaatatgc cgataaaaaa cgcaatcata aataaggtaa acgtaaacaa caattgccag
     2581 cgggagtcat acattttttg tggtaactca tcggtccaaa acgtttgaaa ttttcgtctt
     2641 cttcttactt tatttttata gatggtttga aaaacctttt gggaaagatt gttcaaataa
     2701 attttaaccg aacggtttgg ataataggtg cgggcatagg aaagatcatc ggttacctga
     2761 ataaacaggt tgctcacctt ttcagggtcg gactcttttt cgaatttttg ttcaaattcg
     2821 ccccatttct gcttgttttg ttcaataaaa ctggtttccc gcatgtgttt tctataacgc
     2881 ggtaaatata taggtttatt gcaaggaaat aaaagttcac ctattgcaat tcggaaacaa
     2941 tttaatttct acccaaatcg ttgtatgcat tctgataaaa atgatacatt tgggctttga
     3001 tgtaagcgaa attcacggat gcggacaatt gatatcttaa ctacacaaaa tgtaaccatc
     3061 aattacgagc tggcagaagc caaggatagg atatttgcta ctatcatcga tatcattatc
     3121 caatctatag gtctcttttt cctttctctg atttttatgt ccagcggaat gatgagtgaa
     3181 gactactttt tatatttcat cgtttttccg gtattgtttt tttattattt cggttcagag
     3241 ctgcttatgg atggccaaac ccttggtaaa cggatgttgg gaatcaaagt ggtaaagctg
     3301 acaggcacag aaccctctat aaacgactat ttgatgcgat gggctttaag gccgatagac
     3361 atcttttttt ctttgggttc tataggcata atgctggtaa gttcatcgga taaagcacag
     3421 cgtttaggcg atatagttgc taataccact gtaatcaaaa ccaatccatc tcaaagaatt
     3481 caacttgctg acatactcag tatcagaaac ttatctaatt acgaaccggt atatccgcaa
     3541 attagaactt ttgccgaaca cgaaatgctg ttgttaaagg aagtgatgga caaaagcctt
     3601 aaatatcaga atagtagtca cgaagaagcc ctggaaaccg cttgccaact ggtcaaacaa
     3661 aggctcaatc tcgaaaaaat ccccgccgac aggacaagtt ttatccgcac tttgatcaaa
     3721 gattatgtgg cgctaagtcg ctgattttta ccctattcgc cgtataactg ttttaaaaga
     3781 acgtcaattc gttgtaaatc cttcattttc gggaataaaa gccatacctt tgcacccatt
     3841 ttatgcagga aataagaaat attgcaatta ttgcacacgt tgaccatggc aaaacaaccc
     3901 tggtggacaa aattttacat cattgccagc tcttctcaaa agagcatagt gagctcattc
     3961 tcgacaataa tgaactggaa cgcgaacgtg gtattaccat tgtatcaaaa aacgtttctg
     4021 ttaattacaa aggcttcaaa atcaacatta tagatacacc gggccacgcc gattttggcg
     4081 gggaagttga acgtgtactc aatatggccg atggcgcctt agtgttggtg gatgcttttg
     4141 aaggaccaat gccacaaaca cgttatgtgt taagcaaggc catcgcttta ggattaaaac
     4201 catgtgtagt tatcaataag gtggacaaag aaaactgtaa acctgatgaa gtgcac
//
LOCUS       DAORKW010000312         4249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1008_length_4249_cov_4.994278, whole genome shotgun sequence.
ACCESSION   DAORKW010000312 DAORKW010000000
VERSION     DAORKW010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1008_length_4249_cov_4.994278"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLB46_11535"
     CDS             <1..714
                     /locus_tag="PLB46_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRF77197.1"
                     /translation="RLCKAVGMQSTFIHLDASTTESELLKIVHDINQNEDIDGFIVQL
                     PLPKHINETNIIEAINPKKDVDGFHPINLGRMQLGLPAFISATPAGIVTMLERYQIET
                     SGKNCVVIGRSNIVGTPMSILLSRNTNPGNCTVTICHSRTKNLKEICLQADIIIAALG
                     KPEFVTADMVKEGAVVVDVGISRLDADNEKGYVIKGDVKFDEVAPKCAAISPVPGGVG
                     LMTVVSLLMNTMKAVELNN"
     gene            781..1404
                     /locus_tag="PLB46_11540"
     CDS             781..1404
                     /locus_tag="PLB46_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRF77198.1"
                     /translation="MSNLIYPLAQQYPIMEQFYTIQGEGFHQGRAAYFIRLGGCDVGC
                     VWCDVKESWDAAKHPLISAEAIITEAKKYPSKLVVITGGEPLMYDLHVLINGLQEAGF
                     EVNIETSGAHPMRGSADWICFSPKKFKAPLSEVAAIANELKVVIYNKSDFEWAEEHRK
                     TVGATCKLYLQPEWSKRDIITPLLIEYVKEHPEWEISLQLHKYIQIP"
     gene            complement(1463..3526)
                     /locus_tag="PLB46_11545"
     CDS             complement(1463..3526)
                     /locus_tag="PLB46_11545"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HRF77199.1"
                     /translation="MIHTHTMRRMFMTFVISTLCASVTFAQYCTPIYTTGSAEGDYIN
                     GVAVADLSNTGSGGAITGIGYSDYTGLTANVNIGDTYTMTCTNNPSWSQTYTAWIDYN
                     HDNVFDVSEVIGNLSLAAGASSSFSFTVPTGLIPGNTRMRVRCIYPSGLGTPLDPCAS
                     TTYGEAEDYTLNLMGGLTNDVAVVDITNPTTGPGLGLEDVIVTVENFGSSDASGFTVS
                     YQVDGGLVTAGFFPGTLPAFSTASWTAPEGWDFSAEGCYEITAWTTLAGDEDPSNDSY
                     TETVCHTGAVAGNGAYYILSNTTGGEPWFTVNNSNAMTAVFGTEGVGWTRDYFETVDV
                     PSVFGPDNCFVFLEGSDGHAIELENFLIANISTIESWVASGGKLLLNAAPNEGDGMSF
                     GFGGTSLFYAYFTSNATAAAGAGGHPIFSGPFLPAGTDYTGTSFGHATVTGTGLTNLM
                     VDAFAPSNVVLAEKAWGSGTVLFGGMTTDNWHSPSPNASNLRANIISYLACDVALCPA
                     PIGLGADAITSTSAHLSWDPSDNSGGYHLSVYTLDEVEVLKKKVLGGLTDWTVTGLTP
                     GTDYGFHVRSICVADGIKSVISDHYYFSTPARIGDIENDQIAMYPNPSNGVFTLELNN
                     YEGNNFEVNITNALGQIVYTTMIDVTENNYTQTIDLGNAAAGIYQVTLKNASTTMNYA
                     ISIND"
     gene            complement(3840..>4249)
                     /locus_tag="PLB46_11550"
     CDS             complement(3840..>4249)
                     /locus_tag="PLB46_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77200.1"
                     /translation="GVNSTSYALVDNLTPLTTYAFRIKTVCYDEGLISSPTEWIYWTT
                     LGRIGEEEVGVSLFPNPNNGTFTVNVNGYENNAFTLNVVNAIGQVVYTQPVDINSNEF
                     TETISLENVTPGMYQVNLSNASHNINYSIIITE"
BASE COUNT         1438 a          752 c          756 g         1303 t
ORIGIN      
        1 aggttgtgta aagctgttgg catgcagtct acttttattc atttagatgc ttcaactaca
       61 gaatccgaat tattaaaaat tgtgcatgat atcaatcaaa atgaagatat cgatggtttt
      121 attgttcagc tgcctttacc aaaacatatc aacgaaacaa atataatcga agccattaat
      181 cctaaaaaag atgtggatgg atttcatccc attaatttag gtcgtatgca attgggactt
      241 cccgcattta tttctgcaac accggcaggt atagttacca tgctcgaacg atatcaaatt
      301 gaaacatcag gtaaaaattg tgtcgtgatt ggccgtagta atattgttgg cactccaatg
      361 agtattttat tatcccgaaa taccaatccc ggtaattgca cagtaacaat ttgccatagc
      421 cggactaaaa atttaaaaga aatttgttta caagctgata ttattattgc cgcattaggc
      481 aaacctgaat ttgtaacagc tgacatggta aaagaaggag ctgttgttgt tgatgttgga
      541 atttcacgtt tagatgcaga taatgaaaaa ggttatgtga taaaaggtga cgtgaaattt
      601 gatgaagttg caccaaaatg tgctgctatt tctccggttc cgggaggtgt tggattaatg
      661 acagttgttt cgttattaat gaatactatg aaagctgttg aactcaataa ctaaacaggt
      721 aatctcattc tagtcaataa aatataaggt taagtttaac ttaccaattt gaataaatcg
      781 atgtcgaatt taatatatcc acttgctcaa caatatccca tcatggagca gttttatact
      841 atccagggtg aaggatttca tcagggcaga gcagcgtatt ttattcgcct tggtggttgt
      901 gatgtgggtt gtgtttggtg cgatgtaaaa gagagttggg atgcagccaa acatccgtta
      961 atcagtgcgg aagcaattat tactgaagca aaaaaatatc catctaaatt agttgtaata
     1021 accggtggcg aacccttgat gtatgattta catgttttaa taaatggttt gcaggaagca
     1081 ggttttgaag taaatattga aacttccggt gcacatccaa tgcgtggtag tgcagattgg
     1141 atttgttttt ccccgaaaaa atttaaggct cctttatctg aagttgccgc aattgccaat
     1201 gaattaaaag tggttattta taataaaagc gatttcgagt gggcagagga acatcgtaaa
     1261 acagttgggg caacttgtaa attatatcta caacctgagt ggagtaaacg agatataatt
     1321 acaccgcttt taattgaata tgtaaaagaa catcccgaat gggaaatttc tttacaattg
     1381 cataagtata tccaaattcc ttaataaaaa aaagaggctg ccgaaaacga caacctcttt
     1441 ctatttttaa aatttctgct tatcaatcat taattgaaat ggcatagttc attgtagtgc
     1501 ttgcattttt cagtgttacc tgataaattc ctgcagctgc atttcctaag tcgatagttt
     1561 gagtgtaatt attttcagtt acatcaatca ttgttgtata aacaatttga cctaatgcat
     1621 tggtaatatt tacctcaaag ttatttcctt cataattatt taattccaat gtaaacacac
     1681 cgtttgaagg gtttggatac atggcaattt gatcattttc aatgtcgcca atacgagctg
     1741 gtgttgagaa ataataatgg tcagaaataa ctgatttaat accatcggct acgcaaatag
     1801 aacgaacgtg gaaaccataa tcagtaccag gagttaaacc ggtaactgtc cagtctgtta
     1861 atccacctaa tacttttttc tttaacactt caacttcgtc gagtgtgtaa acagataaat
     1921 gatatccacc tgagttatca gaaggatccc aggataagtg tgcagatgtt gaagtaatgg
     1981 catcagcacc taaaccgatt ggagcaggac ataaagcaac atcacaagct aaataagaaa
     2041 taatatttgc tcttaagtta gatgcatttg gagaagggct atgccagttg tcggtagtca
     2101 taccaccaaa taaaacagtt cctgatcccc aagctttttc tgctaataca acattggaag
     2161 gtgcaaaagc atcaaccatt aaattagtta agccagtacc tgtaactgta gcgtgtccaa
     2221 aacttgtacc tgtataatca gttccagcag gaagaaatgg tccactaaat attgggtgac
     2281 caccagcacc tgctgcagcg gtagcattag aagtaaaata tgcatagaat aaagaagtac
     2341 caccaaaacc gaagctcatt ccatcgcctt catttggtgc tgcatttaat aataatttac
     2401 cacctgaagc aacccatgat tcgatggttg aaatatttgc aatcaagaaa ttttccaatt
     2461 caattgcatg tccatcagaa ccttctaaga atacaaaaca attatcaggt ccaaatacag
     2521 agggtacatc tacagtttca aaataatcgc gtgtccaacc aacaccttca gttccgaaaa
     2581 ctgcagtcat tgcattagag ttatttacag taaaccaagg ttcaccacct gtagtattag
     2641 ataagatata ataagcaccg ttacctgcaa ctgcaccggt atggcaaact gtttcagtgt
     2701 aactatcgtt actaggatct tcatcaccgg caagtgtggt ccatgctgta atttcataac
     2761 aaccttctgc gctaaaatcc cagccttcag gagctgtcca tgaagctgtg ctaaatgcag
     2821 gtaaagttcc agggaaaaat ccggcagtaa ctaaaccacc atctacctga taactaactg
     2881 taaatccgga agcatcactt gaaccaaagt tttcaactgt aacaattaca tcttccaaac
     2941 caagtcctgg acctgttgtt ggattagtaa tgtcaacaac agcaacatcg tttgttaatc
     3001 ctcccattaa attcagagta taatcttcag cttcaccata agttgtagat gcacatgggt
     3061 ccaacggagt acctaaacca cttggataaa tacaacgaac acgcattctg gtgttgccag
     3121 gaattaaacc tgtaggaacg gtaaatgaaa atgaactact tgctccagca gctaaactta
     3181 aattgcctat aacttcagat acatcaaaaa cattatcatg attatagtca atccaggcag
     3241 tgtaagtttg cgaccatgat ggattgttag tacaggtcat agtgtacgta tcaccaatat
     3301 tgacattggc tgttaaacca gtgtaatctg aataaccaat tccggtgata gccccacctg
     3361 agcctgtgtt agataaatcg gctacagcaa cgccattaat gtagtcgcct tctgctgatc
     3421 cggttgtgta aattggtgtg caatactgtg caaaggtgac agatgcacat agggtagaga
     3481 tgacgaatgt cataaacatc cgcctcattg tgtgagtgtg aatcataagc ttgtttttta
     3541 gttaattaat aatccaagct aaaaactaat tttaacaaat gcaaatttaa atagcaatat
     3601 tttttattaa accactgatt atcaagggaa ttgtgacatc ttcagtttaa ttatttatat
     3661 atataaccat ttatattaac ctaagtatat tcctaaataa ttattaaata aaaaaatgag
     3721 tgataaaatt aattaccact cattctaaat tagttattta taagaataat taagcaataa
     3781 aaaaaaatga gtggtaaaat taattaccac tcattttaaa taattattta tttaattaat
     3841 tattcagtaa taataattga ataattaata ttatgcgatg cattactgag gttaacctga
     3901 tacataccag gagttacatt ttctaatgaa attgtttcag taaattcatt gctgttaata
     3961 tcaaccggtt gagtataaac aacctgacca atagcgttta caacatttaa agtaaatgcg
     4021 ttattttcat aaccatttac gtttactgta aatgttccgt tatttggatt agggaataaa
     4081 ctaacaccaa cttcttcttc accgatacgg ccaagtgtag tccagtaaat ccattctgtt
     4141 ggagaagaaa ttaatccttc atcataacaa actgttttaa ttctgaacgc ataagtagtt
     4201 aatggtgtaa ggttatcaac taaagcataa gatgtagagt taacacctt
//
LOCUS       DAORKW010000313         4237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1014_length_4237_cov_4.671210, whole genome shotgun sequence.
ACCESSION   DAORKW010000313 DAORKW010000000
VERSION     DAORKW010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1014_length_4237_cov_4.671210"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PLB46_11555"
     CDS             <1..416
                     /locus_tag="PLB46_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HRF77201.1"
                     /translation="QAKVQEHAGKADVVICTAQIPGRKAPILLLKETVANMKPGSVII
                     DLAASTGGNCEVTENGKTIVVNGVTVIGDSNLQATMPYDASKMFGKNILNYIKLIIDK
                     DGNINLNFADDLVTGTCIAHEGEIKNERVKAVVTA"
     gene            456..773
                     /locus_tag="PLB46_11560"
     CDS             456..773
                     /locus_tag="PLB46_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HRF77202.1"
                     /translation="MILLETTGAFLTTLDTWDIIYILILAAFLGVEVISNVPAILHTP
                     LMSGANAIHGVVIIGSIIVMGHADSTLALVLGFLAVILGTLNVVGGFVVTNRMLQMFK
                     KKK"
     gene            876..2267
                     /locus_tag="PLB46_11565"
     CDS             876..2267
                     /locus_tag="PLB46_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRF77203.1"
                     /translation="MSNLDILKLCYLLGSLSFIVGLKMLGKPDTAKKGNFYAGIGMAI
                     AILGTIFFHQNGEGEKIGNLAWIFGGIAIGTIVGYFAAMKVKMTQMPQMVSLFNGMGG
                     ACAALIGIVEMDTHHPEAFGERFIIFLGLMIGTISFAGSMIAYGKLEGKIKDVGSKFQ
                     QIFNNTFIFAIIALVGYASYDVEFMSSNVIWIIFGASLIYGVLFVMPIGGADMPVVIS
                     LLNSFTGVAAAFGGFLYDNQVMLTGGILVGSAGTILTVLMCKAMNRSLWNVIIGAFST
                     NTSTGKSTEGLSIREINATDTAILCAYAQKVTIIPGYGLAVAQAQHTCHEFEKVLEEK
                     GVEVNFAIHPVAGRMPGHMNVLLAEADVSYDKLKEMEDINPQLPNTDVVIVIGANDVV
                     NPAAETDPGSPIYGMPIIKANTAKNIIVMKRSMAKGYAGIENELFYDSKTRMLFGDAK
                     AMLEKLIAEVKAV"
     assembly_gap    2735..2834
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2960..4195
                     /locus_tag="PLB46_11570"
     CDS             2960..4195
                     /locus_tag="PLB46_11570"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF77204.1"
                     /translation="MNFKLAIIGGGAAGFFAAINAAEMSPAIEVHVFEKAAQFLSKVR
                     VSGGGRCNVTHACFNPKELIQFYPRGSRELLGPFYVFNPADTIEWFEKRRVKIHAEAD
                     GRMFPVTNTSQTIIDCFLQEAEHHNVQLHTQIGLTGITKLDNQQWQLNFNHGGDFIAD
                     AVIFASGSSMPIWEILKQLGHTIITPLPSLFTFHIKDKRIDELMGVSVPDVICKIDGE
                     KISSTGPLLITHWGLSGPAILKLSAWAAVQLAAKQYQFTLKINFLPEHNYQSCLELLI
                     SQKIASPKKHIQLYPIGAVPSRLWKSICLYCGIHDKINWSDASKDMLQKLADSLTQAA
                     FKVTGKSTFKEEFVTCGGVALEEVDLRTMQSKKLPGIYFAGEVLNIDAVTGGFNFQAA
                     WTTAYIAASSITNGFESKK"
BASE COUNT         1309 a          662 c          844 g         1322 t
ORIGIN      
        1 aacaggcaaa agtgcaggaa cacgccggaa aagcggatgt ggttatttgt actgcacaaa
       61 ttccgggtcg taaagcgcct attcttttat taaaagaaac cgttgccaat atgaaaccgg
      121 gttcagtaat tatcgaccta gctgcaagca ctggcggtaa ctgcgaagta accgaaaacg
      181 gaaaaacaat tgttgtaaat ggtgtaacgg ttattggcga tagtaattta caagccacca
      241 tgccttacga tgccagcaaa atgtttggta aaaacatatt gaattacatc aaattgatta
      301 tcgataaaga tggtaatatc aatttaaatt ttgccgacga tttagttaca ggtacttgta
      361 tcgcacacga aggtgaaatt aaaaacgaac gtgtaaaagc agtcgttact gcataatatt
      421 tttagccaac tcaattaacc gaaaataata attgtatgat acttttagaa acaaccggtg
      481 catttttaac aacacttgat acctgggata ttatttatat tttaattctt gccgcttttt
      541 taggggtaga agtaattagc aatgtgcctg ccattttgca tacaccatta atgagtggtg
      601 ccaacgctat tcacggtgtg gtaattatcg gttctattat tgtaatggga catgctgata
      661 gtacgctggc attagtattg ggatttttag ccgtaattct tggaacactc aatgttgtag
      721 ggggatttgt ggttactaat cgtatgttgc aaatgtttaa gaagaaaaag tgaaactaat
      781 gtgctgatgt ggaaatgtgc ttatgtgctg attaacacat ttatacataa aatttgaatt
      841 gaaatataat attataaaga ctaaagatat ttactatgag taatttagat attttaaaac
      901 tttgttacct gcttggcagc ttgagtttta ttgtgggttt aaaaatgtta ggtaagccgg
      961 acactgctaa aaaaggtaat ttttatgccg ggattgggat ggctattgcc attttaggca
     1021 ccatattttt tcatcagaac ggagaaggtg aaaagattgg taacctcgcg tggattttcg
     1081 gtggtattgc aataggaacc attgttggtt actttgctgc catgaaagtt aaaatgacgc
     1141 aaatgcctca aatggtatcg ttatttaacg gaatgggtgg tgcctgtgcg gcattaatcg
     1201 gtatagtgga aatggatacg catcatcctg aagctttcgg tgaacgtttt attattttcc
     1261 ttggattaat gattggtact atttcatttg caggtagtat gattgcctac ggaaaattag
     1321 aaggaaaaat taaagacgta ggaagcaaat ttcagcagat atttaacaac acatttatat
     1381 tcgccattat tgctttggta ggttatgcat cttatgatgt ggaatttatg agcagcaatg
     1441 ttatctggat tatttttggt gcatcattaa tttacggtgt attatttgtt atgccgattg
     1501 gtggagctga tatgccggta gtaatttcat tactcaattc atttaccggt gtggctgctg
     1561 cttttggcgg tttcttatac gacaatcagg taatgcttac cggtggtatt ttggttggta
     1621 gtgcaggtac cattttaacc gtattaatgt gtaaagccat gaacagaagt ttgtggaatg
     1681 taattattgg tgcatttagt actaacacct caacgggaaa atctactgaa ggattatcga
     1741 taagagaaat aaatgcaacg gataccgcaa ttttatgtgc ctatgcacaa aaggttacta
     1801 ttattccggg atacggttta gcagttgcac aagcgcaaca tacttgtcac gaatttgaaa
     1861 aagtattgga agaaaaaggt gtagaagtaa acttcgctat tcacccggtt gcaggtcgta
     1921 tgcctggtca catgaacgtt ttattagcag aagcagatgt ttcttacgat aaattaaaag
     1981 aaatggaaga catcaatcct cagttaccaa acacagatgt ggtaattgta attggtgcaa
     2041 acgatgtggt aaaccctgca gcagaaaccg acccgggttc acctatttat ggaatgccaa
     2101 ttatcaaagc gaataccgct aaaaatatta ttgtcatgaa acgttcaatg gctaaaggtt
     2161 atgccggtat cgaaaatgaa ttattttacg atagcaaaac aagaatgtta tttggcgatg
     2221 ctaaagcaat gctggaaaaa ttgattgctg aggtgaaggc ggtttaatta gctaattagt
     2281 taattagcaa attagctaat ggggtttttg agtggaattt aatttcggat tatgattgga
     2341 gaaattaggt aattagcaaa ttagctgatt agctaattag ctaattgggt tttcgagaag
     2401 catctagttt taccgtatta atagacattt aagctgattc ctatggaatc agctttttta
     2461 tatgtggaaa cggttaattc acgttttatc ctaaatccat catcagctaa tttgctaatc
     2521 agctaattaa ctaattccat aggaaccaac tttttaaatg tggaaccgct tcattcaagg
     2581 tttatcctaa acccatcatt agctaatttg ctaatcaact aatttgctaa tcccatagaa
     2641 atcaactttt taaatgtgga accgcttcat tcacggttca tcctaaatcc atcatcagct
     2701 aatttgctaa tcagctaatt aactaattcc atagnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnttgcta atcccataga aatcaacttt ttaaatgtgg aaccgcttca
     2881 ttcacggttt atcctaaatt catcatcagc taattagcta atttgctaat tagctaatta
     2941 gctaattacc tttacagcca tgaactttaa gcttgcgatt ataggaggtg gtgctgcggg
     3001 attttttgct gctattaatg ctgcagaaat gtcgccggct attgaagtgc atgtatttga
     3061 aaaagcagcg cagtttttaa gcaaagtaag ggtttcaggt ggtggaagat gtaatgttac
     3121 gcacgcttgt ttcaatccaa aagaactcat tcaattttac ccaaggggta gtcgcgaatt
     3181 attgggaccg ttttatgttt ttaatcctgc cgacaccatt gaatggtttg aaaaaaggcg
     3241 ggtaaaaatc catgctgaag ccgatggtcg catgtttccc gttactaata cttcccaaac
     3301 aataattgat tgctttttac aggaagctga acatcacaat gtgcaattac atacacaaat
     3361 tggtttaacg ggcattacaa aattggataa tcagcaatgg caactcaatt ttaatcacgg
     3421 tggagatttt attgctgatg cggtaatttt tgcttcggga agcagtatgc ctatttggga
     3481 aattttaaaa cagttaggac ataccatcat cactccccta cccagtttat ttacttttca
     3541 tattaaggac aagcgtattg atgaactaat gggtgtaagt gtaccggatg ttatttgtaa
     3601 aattgatgga gaaaaaatta gcagtacagg accattatta attacacatt ggggattaag
     3661 cggaccggca atattaaaat tatcggcatg ggctgcggtg caacttgctg caaaacaata
     3721 tcaatttaca ttaaaaatta attttttacc ggagcataat tatcagtcct gcctcgaatt
     3781 gttaatttca caaaaaattg catcacccaa aaaacatatt caattatatc ctattggtgc
     3841 cgtgccatcg agattatgga aatcaatttg tttgtattgc ggcatacacg ataaaattaa
     3901 ctggtcagat gcttccaaag atatgttaca aaaacttgcg gattcgctca cacaagcagc
     3961 atttaaagta acaggcaaaa gtacatttaa agaagaattt gtcacctgtg gcggcgtagc
     4021 attagaagaa gtggatttgc gcactatgca aagcaaaaaa ttgccgggta tttattttgc
     4081 cggcgaggtg ctcaatattg atgccgtaac aggtggattt aattttcagg ctgcctggac
     4141 aactgcctat attgctgcat cgagcattac taatggtttc gaatcaaaaa agtgattact
     4201 tttgtaaata taattctact gttatgaaaa agtttat
//
LOCUS       DAORKW010000314         4213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1018_length_4213_cov_5.291486, whole genome shotgun sequence.
ACCESSION   DAORKW010000314 DAORKW010000000
VERSION     DAORKW010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1018_length_4213_cov_5.291486"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLB46_11575"
     CDS             complement(<1..438)
                     /locus_tag="PLB46_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77205.1"
                     /translation="MGNNKITGVITMMVVGGLLLVLFIGRWLQTEFNTAKLELNKDIF
                     EQFIDAKTRLTDTLIAKNLIQPILDDTAGFKIETYAKKGIDHNGDSVQIITSTTFGDT
                     APNLIEVKGLPHGIEQESFNLEFSTDSSNILFQSVKMFITEVNK"
     gene            568..1311
                     /locus_tag="PLB46_11580"
     CDS             568..1311
                     /locus_tag="PLB46_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HRF77206.1"
                     /translation="MRKQITLCFLLLCTIGAFAQQSGTVQYQETRKLDLHFDGDMPPP
                     PGLPTEHTNKTLLFFNDAASLYKNDTANTDNMNVDRETEDGGRVFIQMAAPDNQIYCD
                     LANKKMIQQRDFMQRKFLIEGEMVAEGYKLTGKQKMILNYPCSEAIKTDSTKTMVLWY
                     TPAIPVSSGPFNYVGLPGLVLEVNINNGEQIITALSVSKEVNPKNIVKPKDGKKVTQE
                     EFDKIVEEKMKEMGVEGGRPGVVIKIEND"
     gene            1321..3990
                     /locus_tag="PLB46_11585"
     CDS             1321..3990
                     /locus_tag="PLB46_11585"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF77207.1"
                     /translation="MKKSIVTIILCWSVFQLFGQQSLNGIVMSDKATPVEFGTVALLK
                     PSDSTLLYFGITNASGNFEIKSVAGGNYVLQISFIGYNTYDTLITVPNSKEGNLGLFI
                     LQTATVGLDAVNISAERIPIQIKGDTIQYNASSYKTHPDASAEDLLKKMPGIEVDQSG
                     NIKAQGEDVQKVLVDGKEFFSNDPTVATKNLPADAIEKVQVYDKSSDEVEFTGIEDGS
                     QSRTINLVLKEGKKSMWLGDAKAGGGTDNKYQVNAKLYRFTKSDQIAFLGMLNNINQF
                     GFSFSDYIDFNGGIGNMMRGGGFRIETEGDLPIDFGQTINGLITSGAAGVNYSHEVVE
                     GNRFNVSYLGNGYDKHLENSTYTENYTPGFNYTTDATDDEVTRNYYHRLNFNLKNKPD
                     SMQSIFGTGGLSLNYGQSNASGFTSTYLADIVTNSLTSSSFDKSDNVSGNANLSYIRK
                     FANDWKYIKITTDLSAKQTLTAAEWDNVTNYFDTGITVADNAFQDETNNNASVNVDLI
                     TLRKIGKSLYLEPVLSGGYSYELLQRSRGNLLEGPTPIDSLSPEFTRKNYFIQPRLSL
                     KRNTKKTQFKFGVGVEYLSMQNMVFDALYPEKTSLYVIPELFYEKEIKSGRRFGINYN
                     AYVNTPTADQLLPVSDDINPLSVYTGNPDLEPEYVHNLYGHFMLFDQFSFTSLFTRAQ
                     LTYVQNKINFSKVINPDLTEEYTVVNVDQDIAGSIGADFGTPIRPLKINLSLDLSESY
                     DRGINYVNGEENLINAFTHRVGLSFDNRKKDYVDARVGGSIKFTQTDYSIETGQSGNY
                     VTNSLYLDLSVTPDSLWRIAFNGEISEYSVTGFEESTIVPLLSAEISRYILKSNRGTI
                     TLKAYDLLDKNTGITARSWSWCSPRPATRRSWR"
     gene            complement(3929..>4213)
                     /locus_tag="PLB46_11590"
     CDS             complement(3929..>4213)
                     /locus_tag="PLB46_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77208.1"
                     /translation="AQGSWWNLKRYFRFNVASAYRVQVESYADEPAVQQQIDKLYGKR
                     RNLTIQSNQTLKSSALNLIGQTSSLFDVLAFIAMIVASLGVVNTMTMNVL"
BASE COUNT         1361 a          683 c          882 g         1287 t
ORIGIN      
        1 cttattcact tctgtaataa acatttttac actttgaaac aaaatattac tggagtcggt
       61 gctaaattcc agattaaaac tttcctgctc aatgccatgc ggtaaacctt ttacttcaat
      121 gagatttgga gctgtatcgc caaatgttgt gctggtaatt atttgcacgg aatcgccatt
      181 gtggtcgata ccttttttag cataggtttc aattttaaat ccagctgtat catccagaat
      241 tggctgtata agattttttg caattaatgt atcagtaagg cgggttttgg cgtcaataaa
      301 ttgttcaaaa atatccttgt ttaattccaa tttggcggta ttaaactcag tttgcaacca
      361 tcgtcctata aacaaaacca ggagtagtcc gccaactacc atcattgtta taaccccggt
      421 tattttatta ttccccataa tttctaccta caaaagtata gtattaaata ttgttaaaat
      481 cggcgcttaa ccttaattaa ccttaattgg ggcagagtta acatgcctct tcatagcagg
      541 ttatactttt gcataaaaca attaacaatg agaaaacaaa taaccttatg ctttttgctg
      601 ctctgcacaa taggtgcttt tgcccagcaa agtgggactg tacaatacca ggaaacaaga
      661 aaacttgacc tccatttcga tggtgatatg cctccgcctc cgggcttgcc tactgaacat
      721 accaataaaa cattattgtt ttttaatgat gctgcttcat tatataaaaa tgatactgcg
      781 aataccgata atatgaacgt tgatcgagaa acagaagatg ggggcagagt atttatccaa
      841 atggctgcac cagataatca aatttattgt gatttagcca ataaaaaaat gatacagcag
      901 cgtgatttta tgcaacgcaa atttttaata gaaggtgaaa tggttgctga aggatataaa
      961 cttaccggca aacaaaaaat gattttaaac tacccttgtt ctgaagccat taaaacggat
     1021 agcacaaaaa cgatggtatt atggtacaca cctgctattc ctgtttctag tggacctttt
     1081 aattatgttg ggttaccggg tttagtatta gaagtgaata ttaataacgg agaacaaatt
     1141 attactgctt tatctgttag caaggaagta aaccctaaaa atattgtgaa acctaaggat
     1201 ggtaaaaaag taactcagga agaatttgat aaaatagtag aagagaagat gaaagaaatg
     1261 ggtgttgaag gtggtcgccc gggtgtggta attaaaattg aaaatgacta aacttaactg
     1321 atgaaaaaaa gtattgtaac cataattttg tgttggtctg ttttccaatt atttggccaa
     1381 caatcactga atggtattgt gatgagtgat aaggcaacac cggtggaatt tggaacggtg
     1441 gcactattaa aaccgagtga ttcaacttta ttgtatttcg gaataaccaa tgcaagtggc
     1501 aatttcgaaa taaaaagtgt tgcaggtggc aattatgtgt tacaaatttc atttattggt
     1561 tacaatactt atgatacttt gattacagtg cctaactcaa aggagggtaa tctcggatta
     1621 tttattttac aaactgccac agttggactt gatgccgtaa atatttctgc tgaacgaatt
     1681 ccgattcaaa taaaagggga tactattcag tataatgctt catcctataa aacacatcct
     1741 gatgcttcag ctgaagattt gttgaaaaaa atgccgggca ttgaagtgga tcaatcaggt
     1801 aatattaaag cgcagggcga agatgttcag aaagtattag tggatggaaa agaatttttt
     1861 agcaatgacc caactgttgc gaccaaaaat ttgcctgctg atgccataga aaaagtacag
     1921 gtttatgata aatcgtcgga tgaagtggaa tttaccggaa tagaagatgg ttcacaaagc
     1981 cgtacgatta atctggtatt aaaagagggg aaaaaaagca tgtggttggg tgatgcgaag
     2041 gctggtggag gaactgacaa taaatatcag gtgaatgcaa aattatatcg atttacaaaa
     2101 tcagatcaga ttgcattttt aggtatgcta aataatatca accaattcgg tttcagtttt
     2161 agtgattata ttgattttaa cggtggcatt gggaatatga tgaggggagg aggattcaga
     2221 attgagacgg agggagattt gccgattgat tttggacaaa ctattaatgg cttaataacc
     2281 agtggtgctg ccggcgtgaa ttattcgcac gaagttgttg aaggcaatag atttaatgtg
     2341 agttatttgg gtaatggata tgataaacac ttagaaaatt ccacttacac tgaaaattat
     2401 acacccggat ttaattatac cactgatgcg acagatgatg aagtaaccag aaattattac
     2461 catcgtttaa attttaattt gaaaaataaa ccggattcca tgcaaagcat ttttggaaca
     2521 ggaggtttat cattgaatta tggtcaatca aatgcttccg gttttacatc cacctattta
     2581 gcggatattg taaccaactc gcttacgtcc agcagtttcg ataaatcaga taatgtgagt
     2641 ggtaatgcca acttgagtta tatccgcaaa tttgcaaatg attggaaata tatcaagata
     2701 actaccgacc tgagtgctaa gcagacatta acagctgctg aatgggataa tgttactaat
     2761 tattttgaca ccggtattac agttgccgat aatgcatttc aagacgaaac aaataacaat
     2821 gccagcgtaa atgtcgattt aattactttg cgaaaaatcg gtaaaagctt atatctggaa
     2881 cctgttttgt ccgggggata ttcgtatgaa ttattgcaac gcagtcgtgg gaatttactg
     2941 gaaggtccaa caccaataga tagtctgagt cccgaattta ctcgtaaaaa ttattttatt
     3001 caaccccggt tgtcgctcaa acgaaatact aaaaaaacac aatttaaatt tggcgttggt
     3061 gttgaatacc tcagtatgca aaatatggtt tttgatgcat tatatcctga aaagacatcc
     3121 ctttatgtga taccggaatt attttatgaa aaagaaataa aatccggccg caggtttggg
     3181 attaattaca atgcctatgt caatacaccg actgctgacc aattattgcc ggtttctgat
     3241 gatattaatc cgttatcagt gtacacaggt aatccggatt tggaacctga atatgtgcac
     3301 aatttgtatg gtcattttat gttatttgac caattttcgt ttaccagttt gtttacaagg
     3361 gctcaattga cttatgtaca gaataaaatc aatttctcaa aagttattaa tcctgattta
     3421 actgaagaat atacggttgt gaatgtggac caggatattg ctggttctat tggagcggat
     3481 tttggaacac ctataagacc attaaaaatt aatttgagtt tggacttgtc tgaatcctat
     3541 gacagaggaa ttaattatgt taatggtgaa gaaaatttaa tcaatgcatt tacacacagg
     3601 gtgggtttga gttttgataa caggaaaaaa gattatgtgg atgcacgggt aggtggttcg
     3661 attaaattta cgcaaactga ttacagcatt gaaacgggac aaagcgggaa ttatgtcacc
     3721 aactccttat atctggattt gagtgtaact ccggatagtt tgtggaggat tgcgtttaat
     3781 ggagagatta gcgaatattc agtgacaggg tttgaagaaa gtacgatagt tccattgttg
     3841 agtgcggaga ttagcaggta tatattaaaa tcgaaccgcg ggacaattac tttgaaggct
     3901 tatgatttat tagataaaaa tacaggtatt acagcacgtt catggtcatg gtgttcacca
     3961 cgcccagcga cgcgacgatc atggcgatga acgccaacac atcgaacaga ctgctggttt
     4021 gcccgatcag gtttagcgcg ctggatttca gcgtctggtt cgattggatg gtcaggttgc
     4081 ggcgcttgcc gtagagtttg tcgatctgtt gttgcacggc gggttcgtcg gcgtatgact
     4141 ccacttgcac acgataagcg ctggcgacgt tgaaccggaa gtatcgtttc aaattccacc
     4201 aactgccctg cgc
//
LOCUS       DAORKW010000315         4205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1023_length_4205_cov_4.737108, whole genome shotgun sequence.
ACCESSION   DAORKW010000315 DAORKW010000000
VERSION     DAORKW010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1023_length_4205_cov_4.737108"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLB46_11595"
     CDS             <1..265
                     /locus_tag="PLB46_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRF77209.1"
                     /translation="GAIVTAKGNDVDYVCRFFAPGFGINEDQATGSIQTTLTNYWSKK
                     LNKKSLTSIQLSQRVGKFFTELQGDRVLISGQTKTYLTGEIEF"
     gene            complement(262..2082)
                     /locus_tag="PLB46_11600"
     CDS             complement(262..2082)
                     /locus_tag="PLB46_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRF77210.1"
                     /translation="MKYYRVIGLIICYFMLLHINAQQIQLPEWSKGVLWYQIFPERFC
                     NGDVTNDPQLADIKGSWPHNDTAAWEISPWTSDWYAMQPWEKENGQDIWYQLQRRRYG
                     GDIQGIINKLDYIQALGVGAIYLNPLFMAPSLHKYDGAMYHHIEPTFGPDPEGDKAII
                     AAENPIDPSTWQWTSADKLALELIQECHKRNIKVIFDGVFNHLGRNSFAFVDVVKNQQ
                     NSIYKDWFTIKSWEDKTKGTTFQYEGWFGVPDLPEIREDSNGIVDGPKQYIFNCTQRW
                     MAPDGVVANGIDGWRLDVAFCVDHDFWKSWRLHVKSINKDAYLTAEVVDKPEVVKPYL
                     YGDEFDAVMNYAFAVIATEFFVNQQSQISVTAFDSLLNELRTIYGYDMSLVMQNLFDS
                     HDTQRFTSFLNNPDVARFRTWGDYFNKTKATNKAYNTNKPNAEAYQKQQLMALFQYTY
                     PGAPMLYYGDEVGMWGANDPDCRKPMIWSDLQYDDEVYNWDGSKKANPDKVFIEKQLL
                     AYYQTLGKLHQLPALQKGDFKPLLIDDVDNIYVYTRSYKGQKVIVILNNSKVKQNIEF
                     VDKQNGTYKEYFTGQQVTAEDGFITISCDGFSGKVLISND"
     gene            2120..2374
                     /locus_tag="PLB46_11605"
     CDS             2120..2374
                     /locus_tag="PLB46_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77211.1"
                     /translation="MRKILLFVTIIALMQELFAQSTPYSVVYDDTMVNSIYITIDPDS
                     LEEMFDNLENEYEYAVQFIYDSPVLKDTLENVGFRLRGNT"
     gene            2402..>4205
                     /locus_tag="PLB46_11610"
     CDS             2402..>4205
                     /locus_tag="PLB46_11610"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRF77212.1"
                     /translation="MSFNTYESGRKFEGAKKLNLIGNHNDPTMSREKIYFDIYNSLGL
                     PERRVSFAKIYINNFYYGLYSLTEEYDDVFLRDRFGDDSGNLYKCVYGSNFEYNGEGQ
                     AAYSSYELLNNESANDKSDLINLTNVLDNTPIADLPCELEKIFNVDQFLKIYALDIST
                     GHWDNYGANQNNFYLYHNNFTGQLEFLSYDCDNVLGVDWFGIDWTERDVYDWNFDDRP
                     MVEKLMQVDEYRDRFSYYLNYIASVAMHPDLLNPHIDSIRELIAPAVVDDILHTFDYG
                     YTYDDFYNGFEQNNIDDHTPYGIKNFIEARDENTLSQVELNNITPIIKYIHHYPLMPS
                     SSDVIEITAFIEDDAAIEEVMLNWSYNNVDFESTTMYDDGTHWDGVSGDGIYGNLLES
                     MFHENETLYYYITASDNAGNMNAYPLCGVDSIYVGFSPEHMVINEFLAINTITLPDEN
                     NVYSDYIELFNPTAANIYLGDKYLSDEFDRPGKFKMPDVILYAGAYALFYADDNPEAG
                     IFHCNFKLDGDKDEIGIFEGPEYYYAIIDTVSFTNQVADQSIGRIPNGSGPFVELNEA
                     SPNQNNEVIIPPIDTAFQQQLLIYGNPASNATTLI"
BASE COUNT         1370 a          763 c          697 g         1375 t
ORIGIN      
        1 aggtgcgatt gtaaccgcta aagggaatga tgtagattat gtttgtcgtt ttttcgcacc
       61 cggttttggc ataaatgaag accaggcaac gggttctatt caaactacac tcacgaatta
      121 ttggagtaaa aagttgaata aaaaatcact tacaagtata caactatcgc aacgtgtagg
      181 taagtttttt accgaattac aaggcgaccg agtattaatt tccggccaaa caaaaacgta
      241 tttgaccgga gaaattgaat tttaatcatt actaattaat actttaccac taaatccatc
      301 acaactaata gtaataaatc catcttcagc tgttacttgt tgaccagtaa aatactcttt
      361 gtatgtaccg ttttgtttgt caacaaattc gatattttgt ttaaccttac tattattgag
      421 tattacaatt actttctgtc ccttataact tctggtataa acataaatat tatcaacatc
      481 atcgattaat aaaggtttga aatcaccttt ttgtaatgca ggtaattgat gtaattttcc
      541 taatgtttga taataggcga gtaattgttt ttcaataaat actttgtccg gattcgcctt
      601 tttactgcca tcccaattat acacctcatc gtcatattgt aaatcactcc aaatcattgg
      661 cttccgacaa tcagggtcat ttgcacccca cataccaact tcatctccat aatacaacat
      721 aggtgctccc ggataagtgt attgaaacaa tgccattaat tgttgttttt gataagcctc
      781 tgcattcggt ttattggtat tatatgcttt atttgtcgct tttgttttat taaaataatc
      841 gccccaggtg cgaaacctgg caacatcagg attatttaaa aatgaagtga atcgttgtgt
      901 gtcgtgacta tcaaataaat tttgcatcac taaactcata tcgtacccat aaattgttcg
      961 gagttcattc aataatgaat caaaagcggt aactgatatt tgtgattgtt gattaacaaa
     1021 aaattcggtg gcaattactg caaaagcata attcattacg gcatcaaatt catcaccata
     1081 taaataaggt ttaactactt ccggtttgtc cactacttca gcagttaaat acgcatcttt
     1141 attaattgat tttacatgta agcgccagct tttccaaaaa tcgtgatcaa cacaaaatgc
     1201 aacatccaaa cgccagccat caataccatt tgctacaaca ccatccggag ccatccaacg
     1261 ttgagtgcaa ttaaaaatat attgttttgg cccatcaact atgccattac tatcttcgcg
     1321 aatttcaggt aaatcgggta caccaaacca accttcgtat tgaaaagtag tgcccttcgt
     1381 tttatcctcc caacttttaa tcgtaaacca atctttgtat atagagtttt gctgattttt
     1441 taccacatca acaaacgcaa aactatttct acccaaatga ttaaatacgc catcaaagat
     1501 tactttgata ttccttttat gacattcctg aataagttct aatgcgagtt tatcagcact
     1561 tgtccattgc cacgtagatg gatctattgg attttctgca gcaattatag ctttatctcc
     1621 ttcagggtca ggtccaaaag tgggttcaat atgatgatac atagcgccat catatttatg
     1681 taaacttggt gccataaata gtggatttaa ataaatggcc ccaacaccaa gcgcctgaat
     1741 gtaatccagt ttattaataa taccttgaat atcaccacca tatctgcgac gttgcaactg
     1801 ataccaaatg tcctgaccgt tttctttttc ccatggctgc atggcatacc agtccgaagt
     1861 ccatggcgaa atttcccatg cagcagtatc attatgcggc cagctgcctt ttatatcggc
     1921 caattgaggg tcgttggtaa cgtcaccatt acaaaaacgc tccggaaaaa tctgatacca
     1981 caaaacacct ttcgaccact caggtaattg aatttgctga gcatttatat gtaataacat
     2041 aaaataacaa ataatcaggc cgataacgcg gtaatatttc atactaaatg ggttaaattt
     2101 gcctaaaaat agttccgaat tgagaaaaat tttacttttt gtaacaataa ttgccctgat
     2161 gcaggaatta tttgcacaat ctactcccta cagtgttgta tacgatgata ctatggttaa
     2221 ctccatttac atcaccattg acccggattc gctggaagaa atgttcgata atttagaaaa
     2281 cgaatatgaa tacgcagttc aatttattta tgattctcct gttttaaaag atacactgga
     2341 aaatgtagga tttcgtttac gcggaaatac ttaattattt tctgcaaaaa aatcatttaa
     2401 aatttctttt aacacttacg agtcaggccg caaatttgaa ggtgctaaaa aacttaatct
     2461 aataggtaat cacaacgacc ctaccatgag tcgtgaaaaa atctattttg atatttataa
     2521 tagtttaggc cttccggaaa gaagagtgag ttttgcaaaa atttatatta ataattttta
     2581 ttacggactt tacagcttaa ccgaagaata tgacgatgtg tttttacgcg accgttttgg
     2641 tgacgattcc ggaaacttat acaaatgtgt atacggcagc aactttgaat ataatggcga
     2701 agggcaagct gcttacagtt catatgaatt attaaataat gaaagcgcta acgataaatc
     2761 agatttgata aacctaacca atgtattaga taatacccca attgcagatt taccatgtga
     2821 actcgaaaaa atatttaatg tagaccaatt cttaaaaatt tatgcattag atattagtac
     2881 aggccattgg gataattatg gcgccaatca aaataatttt tatttatacc ataataattt
     2941 taccggacaa ctcgaatttt taagttacga ttgtgataat gttttaggtg tagattggtt
     3001 tggtatcgac tggactgaaa gagatgtgta tgattggaat tttgacgaca gacccatggt
     3061 tgaaaagctg atgcaggttg atgaataccg cgatcgcttt agttattatc tcaattatat
     3121 tgcatccgtt gccatgcatc cggatttatt aaatccacat atagattcaa ttcgtgaatt
     3181 aatagctccg gcagttgtag atgatatatt acacaccttc gattacggtt atacatacga
     3241 tgacttttat aatggatttg aacaaaataa tattgacgac catacacctt atggaattaa
     3301 aaattttatt gaagcgcgag atgaaaatac cttatcacaa gttgaattaa ataatataac
     3361 gccaattatt aaatacattc atcattatcc tttaatgcca tcgtcaagtg acgttattga
     3421 aataacagca tttatagaag atgacgcagc aatagaagaa gtaatgttaa attggagtta
     3481 taataatgta gattttgaat ccactactat gtacgatgat ggtacgcatt gggatggcgt
     3541 gagtggtgat ggtatttatg gcaatttatt ggaatcgatg ttccatgaaa atgaaactct
     3601 atattattat ataacagctt ctgataatgc aggtaatatg aatgcctatc ctttatgtgg
     3661 tgttgatagt atttatgttg gattttcccc tgagcacatg gtgattaatg aatttttagc
     3721 tattaatacc atcaccttac ctgatgagaa caatgtttat tccgattata tcgaactatt
     3781 taatccaaca gcagctaata tttatttagg tgataaatac ctgagcgatg aatttgatcg
     3841 cccgggaaaa tttaaaatgc ctgatgttat tttatatgca ggtgcttacg cgctctttta
     3901 tgccgatgat aatcctgaag ctgggatctt tcattgtaat tttaagttgg atggcgacaa
     3961 agatgaaatc ggaatttttg agggccccga atattattat gcgataattg ataccgtttc
     4021 attcaccaat caggttgctg atcaatcgat aggtcgaatt ccaaacggct ccggtccttt
     4081 tgttgaacta aacgaagcta gcccaaatca aaataatgaa gtgattatcc caccaattga
     4141 taccgccttt caacaacaat tgctcattta tggcaatccc gcaagtaatg caacaactct
     4201 gatta
//
LOCUS       DAORKW010000316         4198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1028_length_4198_cov_5.361332, whole genome shotgun sequence.
ACCESSION   DAORKW010000316 DAORKW010000000
VERSION     DAORKW010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1028_length_4198_cov_5.361332"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLB46_11615"
     CDS             <1..318
                     /locus_tag="PLB46_11615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF77213.1"
                     /translation="ARLLDAAFYRAASAYKMKQYLSCGIPVTASPVGENNTFLKDGIN
                     GFICNNAAEFYEKITYIHELNQQDYNKMCRAARAGLPDFCVDKYIGELIHVADNLLLT
                     VAP"
     gene            complement(354..932)
                     /locus_tag="PLB46_11620"
     CDS             complement(354..932)
                     /locus_tag="PLB46_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF77214.1"
                     /translation="MKKIIVICSVVLMSLPTFAQTHAGFYGGMNFSNVSMQSPELSAE
                     SRAGYQIGAYARSGNIFYGQLGLEFVSTQSYFTMSDSAGYVFYDKVNWRLLNFPIYAG
                     INLLPIAERLMNVRVYAGPDVSLVLNVPVNELSFTPNDFAKVRIDGTVGAGLDILLFS
                     LDAGYNFGLSNVFSDTYNGKAHYAFVNLGLKF"
     gene            1367..2512
                     /locus_tag="PLB46_11625"
     CDS             1367..2512
                     /locus_tag="PLB46_11625"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HRF77215.1"
                     /translation="MKNRLLVTIVLLTTFCTINAQVTIIDSIMAGGIYRNYRLYVPTA
                     YSDDAPSPLLFNLHGYSSDAFGQAVYANFNPIADTAGFLTVLPNGTLDFFGIRHWNIF
                     GPAGTGVDDVAFLSALIDSLDAEYNIDLNRVYSTGMSNGGFMSHALACELSNRIAAIA
                     SVTGSIDKDRMPFCDPQHNTPVMQIHGTADETVPYDGNFEFLPVDSVVSYWVNQNHCD
                     ATPEFTEVPDIVTIDLCTAEHYVYGSCYKETQVELFKIIDGGHTWPGTAITFLGVTNL
                     DINACREIWRFLSQYRLDELTGIETNPLLKNEINIYPNPFAENIYLNSKNGVIESWLL
                     FDAMGNLVAENDAVDNKLITISGNNLQAGLYYLYVNGEVGMQVVKVE"
     gene            complement(2731..3777)
                     /locus_tag="PLB46_11630"
     CDS             complement(2731..3777)
                     /locus_tag="PLB46_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HRF77216.1"
                     /translation="MTRKPVEYTEYLQLEKILNAQTLESDKENAHAHDEMLFIVIHQA
                     YELWFKQIHFEIDSALTIMRKDAVNDNSAELQTIVHRTSRVITILKLLVQQIDILETM
                     TPLDFHEFRDFLRPASGFQSWQFKMLEAKLGLKFDERHGQQYYLSMLKPEYVQFVKEV
                     ESQKTLLELIDEWLRRMPFFNDAQLWANYHTPNTNDSGYHAFWNDYLKAYASTLVEGE
                     KQNIETFEKIFLSEHKSEHTILSSEAMRNALFISLYRGNPILQMPFQLMQNLLDIDEQ
                     MATWRFRHINMVHRMIGSRVGTGGSSGKEYLQGALNKHYIFKDFAFLSSFLIQRNKLP
                     ELSPELKGRLGFGF"
     gene            complement(3781..>4198)
                     /locus_tag="PLB46_11635"
     CDS             complement(3781..>4198)
                     /locus_tag="PLB46_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77217.1"
                     /translation="GRPIQNPPSKKPTHLPTSFKKSAQIRLTRSIRVPFQHPTKKSAP
                     VCRQAGSFSPHHTKKSAPIRLTRSIRVPFHHTSKKIRSNPFNPFNLCSFSPHPTKKSA
                     PVCRQAGQSATSAPRPAGSVFLFLIFHSNIVFLKKN"
BASE COUNT         1257 a          677 c          912 g         1352 t
ORIGIN      
        1 gcacgcttat tagatgcggc gttttaccgt gcagcatcgg cttataaaat gaaacaatat
       61 ttatcctgtg gcattccagt tactgccagt cctgtaggtg aaaataatac ctttttaaag
      121 gatggcatta acggatttat atgtaataat gctgctgaat tttatgagaa aataacttat
      181 atacatgagt taaatcaaca agattataat aaaatgtgca gggcagcaag agcagggtta
      241 cccgacttct gcgtagataa atacataggt gagttaattc atgttgcgga caacctgctc
      301 ttaactgtag caccctgaca atttttttag attaggaaat tttaaatcag cgtttaaaat
      361 tttaatccca gattaacaaa cgcataatgt gctttgccgt tataggtatc agaaaacaca
      421 ttacttaatc cgaaattata accggcatcc aggctgaata ataatatatc taatccagca
      481 ccaacagtac catcaatgcg caccttcgca aagtcgttgg gggtaaaaga caattcattt
      541 accggaacat tgagcacgag tgacacatca ggtccggcgt atactcttac attcatcaac
      601 ctctccgcta tgggcaaaag gtttataccc gcataaatag gaaagtttaa tagtcgccag
      661 tttactttat cataaaatac gtatccggcc gaatcgctca tggtaaaata actttgtgtt
      721 gaaacaaatt caagaccaag ctgtccataa aaaatatttc cggaacgagc ataggcacct
      781 atttgatatc ctgctctgga ttcagccgat aattcaggag attgcattga gacattagaa
      841 aaattcatac ctccataaaa gccggcatgc gtttgagcaa acgttggtaa actcatgagg
      901 actacactgc atatcacaat aatttttttc ataaccttga gttttagtaa tcgttctctt
      961 tgtatacaaa aaaaattaca ccatactggt atgcctactt aataaatcgg ctgaaaatgc
     1021 tactatactt cactttcaga ctgtagttgt taattatttt gtttcaaaca ataaccagct
     1081 aaattgggga attatccctc atattggtta aagatgacac ttaaacaggt taaaataaat
     1141 ggtggatgta tttaattgtt taaattaaca tatgcaatat ttccaatcac ttgtagcata
     1201 aattcaggta ttcaattaac gtgtatgctg gaaaaaatca atttaaataa taaaattggt
     1261 ttaaaggtta ttggattaga ttatttataa ggttcttccc gttcttataa aagattaaac
     1321 ttaaaaccgt aaaaactgtt aactttacta tatcacaaat actattatga aaaacaggct
     1381 actagttacc attgttttat tgaccacctt ttgcacaatt aacgcgcaag ttactatcat
     1441 agatagtatt atggctggtg gtatttatcg aaattatcgg ttatatgttc ctactgcata
     1501 ttcagacgat gcaccttccc cattgttgtt taatttacac gggtattcat ctgatgcatt
     1561 tggacaggca gtgtatgcca actttaaccc aatagctgac acggcaggtt ttttaacggt
     1621 attgcccaat ggcacattag atttttttgg tattcgccac tggaacattt ttggccctgc
     1681 cggaaccggt gttgatgatg ttgcattttt aagcgcatta attgatagtc tggatgccga
     1741 gtataatata gatttaaatc gcgtgtattc cacaggaatg agtaatggtg gatttatgag
     1801 tcatgccctt gcttgtgaat taagtaatcg tattgctgca attgctagtg taaccggaag
     1861 tattgataaa gacagaatgc ctttttgcga cccgcaacat aatactcctg taatgcaaat
     1921 tcatggcact gcggatgaaa cagttcctta tgatggtaat ttcgaatttt tacctgtaga
     1981 ctctgtagta agttattggg tgaatcaaaa tcattgtgat gcaactcctg aatttacaga
     2041 agtgccggat attgtaacta tcgatttatg tactgcggaa cattatgttt atggcagttg
     2101 ctacaaagaa acacaggtgg aattatttaa aatcatagat ggtggtcata cctggccggg
     2161 aactgcaatt acatttttgg gtgttacgaa tttagatatt aatgcgtgta gagaaatttg
     2221 gcgattttta agtcagtatc gtttagatga actgacagga attgaaacaa atcctttgtt
     2281 aaaaaatgaa attaatattt atccgaatcc gtttgctgaa aatatttatt taaatagtaa
     2341 aaatggggta attgaatcgt ggctgttgtt tgatgcgatg ggaaatttag tggctgaaaa
     2401 tgatgctgtt gataataaat taattacgat ttcagggaat aatttgcaag cggggttgta
     2461 ttatttatat gtgaatggtg aggttgggat gcaggtggtg aaggtggaat gatgaggaag
     2521 aggaacctgc ctggcggcag acaggcactg gttgaacggg ttgaacggat gcggacggat
     2581 ttttttggtg ggatgtttta tatgtgggtt gaaaagatgc ggacggattt tttttatttt
     2641 attcgataat aaaattaaat cttatttgta ggagcgaatt gcatccgccg ctacaaataa
     2701 gatttacgaa tttataagat aaatgttttt ttaaaatcca aacccaagtc taccctttaa
     2761 ctccggcgat aattcaggta gtttattgcg ctgaattagg aagctgctta aaaaagcaaa
     2821 atctttaaaa atataatgtt tgttgagtgc cccttgtaaa tattcttttc cggaacttcc
     2881 gccggtgccc acgcgggagc cgatcatgcg gtgtaccata ttgatatgac ggaatcgcca
     2941 ggtagccatt tgttcgtcga tatccaataa attttgcata agttgaaatg gcatttgtaa
     3001 aataggattg ccgcgataca atgaaataaa taatgcatta cgcattgctt cactgcttaa
     3061 tatggtatgt tcacttttat gttcgcttaa aaatattttt tcgaaggttt cgatattttg
     3121 tttttcacct tcaactaatg tagatgcata ggcttttaaa taatcgttcc aaaatgcgtg
     3181 atagccggaa tcgttggtat ttggtgtgtg ataatttgcc cacaattgtg catcattaaa
     3241 aaacggcata cgccttaacc attcgtcgat taattccaat aatgtttttt gcgattctac
     3301 ttcttttaca aactgcacat attccggttt gagcatactt aaataatatt gctgaccgtg
     3361 acgctcatcg aattttaatc cgagtttagc ttccagcatt ttaaattgcc agctttgaaa
     3421 tccggatgcg ggacgcaaaa aatcgcggaa ctcatgaaaa tcgagtggtg tcatggtttc
     3481 gagaatatcg atttgttgca ccaataattt caaaatggtg atgacacgcg aagtgcgatg
     3541 tacgatggtt tgtaattctg ctgaattatc gtttacggca tctttacgca tgatggtaag
     3601 tgctgaatcg atttcgaagt gaatttgttt gaaccataat tcatacgcct gatgaattac
     3661 gatgaataac atttcgtcgt gggcatgggc attttcttta tcgctttcaa gggtttgggc
     3721 gtttaatatc ttttcgagtt ggaggtattc ggtatattcg acaggtttgc gggtcattgg
     3781 tcaatttttt ttcaaaaata caatgtttga gtggaaaatg aggaaaagga acacggaccc
     3841 cgcggggcgg ggcgcggatg tggcggattg gcctgcctgg cggcagacag gcgcggattt
     3901 tttggtgggg tgtggtgaaa aggaacacag attgaacggg ttgaacggat ttgagcggat
     3961 ttttttagat gtgtggtgaa aaggaacgcg gattgaacgg gttaaacgga ttggtgcgga
     4021 ttttttggta tggtgtggtg aaaaggaacc tgcctggcgg cagacaggcg cggatttttt
     4081 ggtggggtgt tgaaaaggaa cgcggattga acgggttaaa cggatttgcg cggatttttt
     4141 gaatgatgta gggaggtgtg ttggtttttt agatggcggg ttttggatag gcctgcct
//
LOCUS       DAORKW010000317         4191 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1033_length_4191_cov_4.564797, whole genome shotgun sequence.
ACCESSION   DAORKW010000317 DAORKW010000000
VERSION     DAORKW010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1033_length_4191_cov_4.564797"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLB46_11640"
     CDS             <1..274
                     /locus_tag="PLB46_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77218.1"
                     /translation="CVSESKTGSRCSSAKTATSSASASNTATEVKTAGACCSGNTAGA
                     EAKATGANCSAHTDAASAKTEGANCHSATMQMGAAQPAKVEETPEK"
     gene            complement(506..1198)
                     /locus_tag="PLB46_11645"
     CDS             complement(506..1198)
                     /locus_tag="PLB46_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HRF77219.1"
                     /translation="MQQLKHIIFDLDNTLWDFSGNSKRILAEIFEKFNLKEAGIPDFE
                     NFHKQYKFRNEYLWNQYALGNVSKEEVRLNRFYASFNDFGINNYVLANQIADHYIYHT
                     RRQTDLLPNAKALLDHLKTKYQLHIITNGFDEVQFFKLENTGLHTYFKTVTTAEAANA
                     LKPDIRIFEHALKAMQTEPQHCLYIGDSPEVDGNGAINAGMHFIWLNTEKKENKFQFE
                     EISDLGELLDIL"
     gene            complement(1198..2208)
                     /locus_tag="PLB46_11650"
     CDS             complement(1198..2208)
                     /locus_tag="PLB46_11650"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF77220.1"
                     /translation="MILVTGANGLVGSYLCRYLIMQGQHVRALKRPDSNLRLVSDIAD
                     KIEWVDGDVNDVMSLESAMLGIEKVYHCAAIISYSKKNADKLMLVNVEGTANVVNIAL
                     EFGVKKLVHLSSIAALGKTGRDGETVAEDTPWERKNFTSNYAISKFLAEREVWRAIAE
                     GLNAVIINPSIIVGAGNWDSGSCKLFTTVYNGFKYYTEGVTGYVDVRDVVKIATQLME
                     SDISEQRFVVNSENVMYRDFLQTIGAGLNVQGPTVKAGKNLSALAWRVEWVKSLFNGK
                     EPSVTKQTAAIANKKVFYNNEKIKQTLGYQFIPIRESITDAAKCFLEEKKDNQFHPVS
                     FK"
     gene            complement(2264..3349)
                     /locus_tag="PLB46_11655"
     CDS             complement(2264..3349)
                     /locus_tag="PLB46_11655"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRF77221.1"
                     /translation="MSKQTEYHILGLMSGSSLDGIDMAYCSIQTNHPYTFTIIAAATI
                     PFTNEEYLMLKNCSPDIHYNYHKEDELFAKVSANAINNFLQKNNLPKPDAIASHGHTV
                     FHFPALGKTKQIGNGQLIANLTSCKVIYDFRTSDVAAGGQGAPLVPLSDAIFFNNYHA
                     CLNIGGIANISFDTPSGRIGFDICGANQLLNYIAQTIDLPFDENGELAAAGFVDEQLL
                     SDLDACTYFQLPYPKSLDNHFVRKYFIERIAAANISVNDKLATATYHIAKQITHVIKQ
                     QNIAQENYRLLVTGGGAFNAYLIQLIEKLAGIKITLPSNEIIQFKEALAMCLMGVEKL
                     QNKPNFLPSVTGAKIAVSGGVVVVPEK"
     gene            complement(3357..3686)
                     /locus_tag="PLB46_11660"
     CDS             complement(3357..3686)
                     /locus_tag="PLB46_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRF77222.1"
                     /translation="MKKLLIFFMAVATILLSNCTSQPSNNGNKPMVKTMTPGEEVYVK
                     HCKLCHGASGDLGLSGAANLKISVLNNAEIITVVTDGRKAMPGWKAQLTPTQINDVAA
                     YVITLRN"
     gene            complement(3741..>4191)
                     /locus_tag="PLB46_11665"
     CDS             complement(3741..>4191)
                     /locus_tag="PLB46_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF77223.1"
                     /translation="GDFFWTPGNLILPPANISSFTEQDPTVELTETTTFILTVTSPDG
                     CVEKDTVTIFVESYTILEVPNAFSPNGDGFNDAIYILTHDIAELLEYSIFNRYGELVF
                     TTTNITTGWNGIVDGKEAEMGTYIYLVRAYDFGGNFVQRQGNIVLVR"
BASE COUNT         1266 a          815 c          713 g         1397 t
ORIGIN      
        1 atgtgtttca gaatcgaaaa caggttctcg ctgcagcagt gcaaaaactg caacgtcgtc
       61 tgcgtctgca agtaatactg ctacagaagt aaaaacagca ggtgcatgtt gtagcggaaa
      121 tactgcaggt gccgaagcaa aagctacagg tgcaaattgt agtgcacata cagatgctgc
      181 ttcagcaaaa actgaaggtg ctaattgtca ttcagctact atgcaaatgg gcgctgctca
      241 acccgcaaaa gtggaagaaa caccagaaaa ataattatta acttatatta taaaatcctg
      301 cagcaatgcg ggattttttt ttgcctgtta gttccaatca attattgtct acacatttac
      361 aattagttca acaatttact cggaaaatca aattactttt tttattgctg caacttaagc
      421 tttgagatat ttttttgtaa taaatgaatg cagagaaatg tgcagctagt taaaaaataa
      481 tcgttacgac tacttattgt attttttaca atatgtctaa taattccccc aaatctgaaa
      541 tctcttcaaa ttggaatttg ttttcttttt tttccgtatt caaccagatg aaatgcatgc
      601 ctgcattaat ggcgccgttg ccatccacct cgggtgaatc gccaatatac aaacaatgtt
      661 gtggttcggt ttgcatggct tttagcgcgt gctcaaatat gcgaatatca ggctttaaag
      721 cattggcagc ttcggcagtt gttactgttt taaaataggt atgcagtccg gtattttcta
      781 atttaaaaaa ttgcacctca tcaaatccat tggttattat atgcagctga tatttagttt
      841 ttaaatggtc taataacgct ttggcattgg gcaacaaatc ggtttggcga cgcgtgtggt
      901 aaatgtaatg gtcggcaatc tgatttgcca gcacataatt attaatacca aaatcgttga
      961 atgaagcata aaaacgattt aatcgcactt cctcttttga tacatttcct aatgcatatt
     1021 gattccacaa atattcattt ctgaatttat attgcttgtg aaaattttca aagtcgggaa
     1081 taccggcttc ctttaaattg aatttttcaa atatttcagc gagaatacgt ttagagtttc
     1141 cggaaaaatc ccacaaagta ttgtctaaat caaatatgat atgtttaagt tgttgcatta
     1201 tttaaatgat acgggatgga actgattgtc ttttttttct tcgagaaaac attttgcggc
     1261 atcggttata ctctcacgaa taggtataaa ctgataaccc aaagtttgtt ttattttttc
     1321 attattataa aacacttttt tattagcaat agcagccgtt tgtttagtaa cagaaggttc
     1381 ttttccatta aacagtgatt tcacccattc tactctccag gcgagtgccg aaagattttt
     1441 tcctgctttt accgttggtc cctgcacatt tagtcctgct ccaatagttt gtaaaaaatc
     1501 gcgatacatc acattctccg aattcacaac aaatcgttgt tctgaaatat cactttccat
     1561 tagttgtgtg gcaattttaa ccacatcgcg cacatcaaca tatcctgtta cgccttctgt
     1621 ataatattta aaaccattat aaaccgttgt aaataattta catgatccgc tatcccaatt
     1681 tccagcacca acaattatag atggattaat aataaccgca tttaatcctt cagcaatagc
     1741 ccgccacact tcacgttctg ctaaaaattt actgatggca taattggaag taaaattttt
     1801 gcgttcccat ggtgtatctt cagcaactgt ttcgccatcg cggcctgttt ttccaagtgc
     1861 cgcaattgaa cttaaatgca ctaatttttt caccccaaac tccaatgcta tgtttaccac
     1921 atttgcagtg ccttcaacat ttaccagcat gagtttatcc gcattttttt tgctgtaaga
     1981 aataatggct gcacagtgat atactttttc tattcccaac atagcagatt ccaaactcat
     2041 cacatcgttg acatcaccat ctacccattc aattttatcg gcaatatcgg ataccaatcg
     2101 caaattacta tcggggcgtt ttaatgcacg aacgtgctga ccttgcataa ttaaatatct
     2161 gcacaggtag ctgcctacta aaccatttgc gccggtcact aaaatcatga atgcaagata
     2221 attgtatgta tgaacatttt atgtattaac ctgaatttta atcctatttt tcgggtacaa
     2281 ccactacccc accactaact gcaatttttg ctcctgttac gctaggcaaa aaattgggtt
     2341 tattttgtaa tttttccacg cccattaaac acattgctaa tgcctcttta aattgaatta
     2401 tttcgttgct tggtaatgtt attttaatcc ctgctaattt ttctatcaat tgtattaaat
     2461 acgcattaaa tgccccgcct ccggtaacta aaagtcggta attttcttgt gctatatttt
     2521 gttgtttaat aacatgtgta atttgtttgg caatatgata agttgctgtg gccagcttgt
     2581 cgtttaccga aatgttagcc gctgcgatgc gttcgataaa atatttcctc acaaaatgat
     2641 tatccagcga tttaggataa ggcaattgaa aataagtgca agcatctaaa tctgacaata
     2701 actgttcatc tacaaaaccg gctgcggcaa gttcaccatt ttcatcaaat ggtaaatcaa
     2761 tagtttgagc aatgtaattc agcaattgat tggcgccaca aatatcaaat ccaattctgc
     2821 ctgatggtgt atcaaatgag atgtttgcaa tgccgccaat atttaaacaa gcgtgataat
     2881 tattaaaaaa aatggcatca cttaaaggca ctaaaggcgc tccctgtcca ccagctgcaa
     2941 catcggaagt acggaaatcg taaatcactt tacaactcgt taagttggca atgagttgtc
     3001 cgttgccaat ttgttttgtt ttacctaaag cgggaaaatg aaataccgta tgtccgtgcg
     3061 aagcaattgc atcgggtttt ggtaaattat ttttttggag gaaattatta attgcattag
     3121 ctgaaacttt tgcaaacagt tcatcctctt tgtggtaatt ataatgaata tcaggtgagc
     3181 aatttttcag catcagatat tcctcgttgg taaacgggat tgtagctgca gcaataatgg
     3241 taaaagtgta tggatgattt gtctgaatac tgcaatacgc catatcaatg ccatcgagtg
     3301 aacttcccga catcaggcct aggatatggt attcggtttg tttactcaac tggtaattaa
     3361 tttctcaatg taattacata agctgccaca tcatttattt gagttggggt aagttgtgct
     3421 ttccaacccg gcattgcctt tcttccatcg gttaccacgg taataatttc ggcattattg
     3481 agaacagaaa tttttaggtt tgctgcgcca ctcaatccta aatcgccact tgctccatga
     3541 cataatttgc aatgttttac atatacttct tcaccgggag tcatcgtttt taccataggt
     3601 ttattgccat tattagaagg ctggcttgta cagtttgaca ataaaattgt ggcaactgcc
     3661 ataaaaaaaa taagtagctt tttcatgctg aaataaattc agtgcaaagc tacttattcg
     3721 agtataaaaa atacgatgat ttacctcact aaaacgatat ttccctgtcg ttgtacaaaa
     3781 ttgcctccaa aatcgtaggc cctaaccaga tatatatagg ttcccatttc tgcttctttt
     3841 ccatcaacta tgccgttcca gcctgtagtg atattcgttg ttgtaaatac caactcccca
     3901 tatctgttaa atattgaata ttctaacaat tcggctatat catgtgtaag aatatagata
     3961 gcatcattaa acccatctcc atttggtgaa aatgcattag gcacttctaa aatggtatat
     4021 gactcaacaa aaatggttac tgtatctttt tctacgcaac cgtccggact agttactgtt
     4081 aaaatgaaag tagttgtttc tgttaattca actgttggat cctgttcggt aaaggaagaa
     4141 atattagcag gtggtaaaat taagttaccc ggggtccaga aaaagtctcc a
//
LOCUS       DAORKW010000318         4181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1038_length_4181_cov_4.316287, whole genome shotgun sequence.
ACCESSION   DAORKW010000318 DAORKW010000000
VERSION     DAORKW010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1038_length_4181_cov_4.316287"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PLB46_11670"
     CDS             <1..898
                     /locus_tag="PLB46_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77224.1"
                     /translation="YMASYSSTCEFLWAHELDVPWYGSGRSVVTDNTGNVYFAGTFLG
                     EVDFNMDDVASNPDTTLGYVGYLVKYLYDGTYVWHALVEGTAVVDCYDVSLDKLGGVN
                     ICGFHAGETDFNYASGAAYVLTADGTNNFVANYSTSGVFKWAFQIYGTTSGVAYCSTN
                     DNVGDLYVTGWIKGTGDFNPDPGITDTIVWSGGEGDFFLAKYITDKQPQSITDFSKNS
                     VLHVYPNPATNVVFIDIDAENMTASHGILSLTNMNGQLIQYQNWNGGLHNDMNLPADL
                     PSGLYLLQLTTSGENFYNVIVH"
     gene            1406..2483
                     /gene="prfB"
                     /locus_tag="PLB46_11675"
     CDS             join(1406..1465,1467..2483)
                     /gene="prfB"
                     /locus_tag="PLB46_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217450.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRF77225.1"
                     /translation="MTFEHLTDLKEKLLVLRRFLNFDDKVIQVNEDEQITHQPGFWDD
                     PKNAEVVLKRIKINNIWINSYNDAATAVDDLGVLYEFSQAGEATEEELEAQYTKTKEV
                     LDELEFKSTLTEQEDQLPAILTINSGAGGTESCDWASMIYRMYNMWASKNGFKVTELD
                     FQAGDVAGIKSASLQIEGEFAYGLLKSENGVHRLVRISPFDSNAKRHTSFASVFVSPL
                     IDDTIEINISPSDLTWDTFRSGGAGGQNVNKVETAVRVTHLPTGIVVACQVNRTQGLN
                     REMAMQMLRSKLYDLELQKRQEVKDKIEGTKKKIEWGSQIRNYVLHPYKLVKDARTGY
                     ETGNADAVLNGGLDEFIKAYLLME"
     gene            2626..3396
                     /locus_tag="PLB46_11680"
     CDS             2626..3396
                     /locus_tag="PLB46_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77226.1"
                     /translation="MKIKHTLLSVLFLIITAFATRAQSVVLCESYDTQGKASGVYDSW
                     SVSATASYIYILYNQPTAMKDGLWYIYIDEYDIAQGKYIPYQTIPITPVANSTWYAHD
                     QQFPDAGSFKVSVMFNGTQMAEFKYSIEVIPSTTSGTDTLTTFYYENSFVTSGTSVTA
                     EGLLVGEAGTFYLNGNASVTVTVYVDNNLKPFLSDFVYVDIFKSPDEEMYDSFTITMQ
                     PDWDYLHFKYEFKEPGTYFVDIFNAQDIFINTGTVIIE"
     gene            complement(3421..3768)
                     /locus_tag="PLB46_11685"
     CDS             complement(3421..3768)
                     /locus_tag="PLB46_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77227.1"
                     /translation="MAAKQRKELSGLYFHFMPNNTSQHILNTAANLLGFCLFVITSLH
                     ITSKAEAQIIDELTSIIAVILTVACLFSFFAIRTAHPKRELQLEKIADYCFVSALVGI
                     LIIILLITLNFIK"
     gene            complement(3772..>4181)
                     /locus_tag="PLB46_11690"
     CDS             complement(3772..>4181)
                     /locus_tag="PLB46_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77228.1"
                     /translation="DKYVGVRFTKDDNFHYGWVRLSIDATTRTPTLTGFAYESEPNIS
                     LKTGTEQIAIQNLFTLKGNVYIEGQNIYITMDKPLDNGSRLKIISLEGKVLMTRMVNG
                     SEIQLEQPAYNAGVYFITIHSTEGDFIHKMILN"
BASE COUNT         1308 a          691 c          849 g         1333 t
ORIGIN      
        1 ctatatggcg agttattctt ctacctgtga atttttatgg gcacatgaat tagatgtacc
       61 ctggtatgga agtgggcgtt cagtagttac ggataatacc ggcaatgttt attttgcggg
      121 tacatttttg ggtgaagtcg attttaatat ggatgatgta gcttccaatc ctgataccac
      181 cttaggttat gtagggtatt tagtgaaata tttgtatgat ggcacttatg tgtggcatgc
      241 gttggttgaa ggaacagccg tggtagattg ttacgatgtg tccttagata aattaggtgg
      301 agtaaatatt tgtggttttc acgccggtga aaccgatttt aattatgcat ctggtgcagc
      361 atatgtatta actgcagatg gcacaaataa ttttgtcgcc aattattcca cttcaggtgt
      421 atttaaatgg gcattccaaa tttatggaac aacatcaggt gttgcctatt gtagtactaa
      481 tgataatgtt ggtgacttat atgtaaccgg ttggataaaa ggaacaggcg attttaatcc
      541 cgatcctggt attaccgata ccattgtttg gtcgggaggc gaaggtgatt tttttcttgc
      601 gaagtatatc acagataagc aaccgcaatc tattacagat ttttctaaaa attctgtctt
      661 acatgtttat ccaaatccgg caactaatgt agtttttatt gatattgatg ctgaaaacat
      721 gacagcatca catggcattt taagtcttac caatatgaac ggacaactca tccaatatca
      781 aaactggaat ggaggacttc ataatgatat gaacttacct gctgaccttc cctcgggttt
      841 atatcttttg caattaacca cttcaggtga aaatttttat aatgtgattg tgcattgagg
      901 gtggtggaat tagctaatta gcaaattagc tgattagcta atggggtttt gtattcggag
      961 tacattttga gtaatgcagg gaatgagcat ttgattaggt aattagctga ttagcaaatt
     1021 agctgatggg gttttgtatt cggagtacat tttgagtaat gcagggagtg tgcaattgat
     1081 taggtaatta gctgattagc aaattagctg atggggtttt gtattcgcag cacattttga
     1141 gtaatgcagg gaatgagcaa ttgattaggt aattagcaaa ttagctgatt agctgatggg
     1201 ggtttttcgt aaaagagcat ttagaatgta gcaggaaatt actacggcgc aaagtatctt
     1261 tgtttttttt taacaatcat tattttttgc aaaaaaataa cccatccatc catataaaca
     1321 acaatgtgcc gtcgcagacg cacatcattt gctaatttgc taatcagcta atttgctaat
     1381 tgcctaattt ccctatcttt gccgcatgac atttgaacat ttaacagatt taaaagaaaa
     1441 actgcttgtt ttgaggaggt ttctttaact tcgatgacaa ggttattcag gtaaacgaag
     1501 acgaacaaat tacacaccag ccgggctttt gggacgaccc taaaaatgca gaggttgtac
     1561 tgaagcgaat caagattaac aatatctgga ttaatagcta caacgatgcc gcaacagctg
     1621 ttgacgacct gggcgtgtta tacgaatttt cacaagccgg agaagcaact gaggaagagt
     1681 tggaagcaca atacactaaa accaaagaag ttttagatga attggaattt aaatccacac
     1741 tcaccgaaca ggaagatcag ctacctgcca ttcttactat caattcgggt gcaggcggaa
     1801 cggaaagttg cgactgggca agtatgatat accgcatgta taatatgtgg gcttctaaaa
     1861 atggctttaa agtaactgag ctcgattttc aggctggtga tgttgcagga ataaaaagtg
     1921 cttctttgca aattgaaggt gaatttgcct atggtttgct caaaagtgaa aatggtgtgc
     1981 accgtttggt gagaatttca ccattcgaca gcaatgcgaa aaggcatacc agttttgcca
     2041 gcgtgtttgt atcgccactt atcgacgata ccattgaaat taatatttct ccttcagatt
     2101 taacatggga caccttccga agcggtggtg caggtggtca gaacgtaaac aaggtggaaa
     2161 cagcagttcg ggttacccac ttacctaccg gtatagttgt tgcctgccag gttaaccgta
     2221 cacaaggttt aaaccgtgaa atggcaatgc aaatgctgcg tagtaaattg tatgacctgg
     2281 aattgcaaaa acgccaggag gttaaggata aaattgaagg caccaaaaag aaaattgagt
     2341 ggggaagtca gatacgcaat tatgtattac acccatataa attggtaaaa gatgcccgaa
     2401 ccggatatga aacaggcaat gcagatgctg ttttaaacgg gggtttggac gaatttatca
     2461 aggcctattt gttgatggaa taaaagtttt tacaataaat ttaaaaacct gaataagcgc
     2521 attttattca ggttttttct tttttttaac caatgggcaa ttttttcgac ttatatcatt
     2581 gattattagg atgattattg gttaatttta gccaaaattg agaatatgaa aatcaaacac
     2641 accctccttt ctgttttatt cctcataatc acagcgtttg caaccagagc ccaatcggta
     2701 gtactttgtg aatcgtatga tacacaggga aaagccagtg gtgtttacga cagttggtca
     2761 gtatcggcaa ctgcctcata catctatatt ttatataacc aaccaaccgc gatgaaggat
     2821 ggtctttggt atatttatat tgatgaatat gatatcgctc aaggcaaata cattccttat
     2881 caaacaatac ccataactcc ggttgcaaat tcaacctggt acgcacacga ccagcaattt
     2941 cctgatgcag gaagttttaa agtttctgta atgtttaacg gaacacaaat ggctgagttt
     3001 aaatatagta tcgaagtaat tccttccact acatctggaa ctgatacttt aactactttt
     3061 tattatgaaa attcttttgt tacatccgga acatcagtaa ctgcagaagg attattagtg
     3121 ggcgaagcag gaacttttta tttaaatggc aatgcatcag ttactgtaac tgtttatgta
     3181 gataataatt taaagccttt tttatctgat ttcgtttatg tagatatttt taaaagccct
     3241 gatgaagaga tgtatgatag ttttactatt accatgcaac ctgattggga ttatttacat
     3301 ttcaaatatg aatttaaaga accaggtact tacttcgtag atatatttaa cgctcaggat
     3361 attttcatta atacaggaac ggtaattata gagtaagtta ataaattatc atcttgctga
     3421 ttacttgata aaatttaaag taatcagtaa gatgataatt aaaatgccaa ctaatgccga
     3481 tacaaaacaa tagtcggcaa ttttctccag ttgtagttct cgtttaggat gagctgtccg
     3541 aattgcaaaa aatgaaaaca aacatgcaac tgtcaatata actgctatta ttgacgtcaa
     3601 ctcatctatt atttgcgctt cagccttact ggtgatatgc aatgaggtga tcacaaataa
     3661 acaaaacccc agcaggtttg ccgctgtatt taagatatgc tgtgaggtgt tgtttggcat
     3721 aaaatggaaa tataacccag ataattcttt cctttgttta gcagccaaaa atcaattcaa
     3781 aatcatttta tggataaaat caccttcagt tgaatgaatg gtgatgaaat aaacgccggc
     3841 attgtaagcg ggttgttcaa gttggatttc gcttccattt accattctag tcataagcac
     3901 tttaccctca agtgaaataa ttttaagcct tgaaccattg tcaagtggtt tatccatagt
     3961 gatataaatg ttttggcctt ctatgtatac gttgccttta agtgtaaaaa gattttgaat
     4021 ggcaatttgt tctgtcccag ttttcaaact tatattaggc tcagattcat atgcaaatcc
     4081 ggtcaaagta ggtgtacgag tagtagcatc aatactaagg cgtacccaac cataatgaaa
     4141 attatcatct tttgtaaatc gcaccccaac atatttatca g
//
LOCUS       DAORKW010000319         4180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1039_length_4180_cov_5.451394, whole genome shotgun sequence.
ACCESSION   DAORKW010000319 DAORKW010000000
VERSION     DAORKW010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1039_length_4180_cov_5.451394"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLB46_11695"
     CDS             <1..825
                     /locus_tag="PLB46_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77229.1"
                     /translation="LLKEISDHLKENSYEEYPSITIYYQILMTFTDTDTEVHFEELKK
                     LLDEHTTKFPPDEARDMYAYAQNFAIRKINAGEKRFLREYFDLSKAALEKELLMVDGD
                     LSPWTFKNLVIAALRVGEFNWAEQFIKNYKNRLNEKFRSNAYNYNQGALLFFKGQYGD
                     ALRLINQVEYTDIFYALDTRTMQIKIYYQLDEWDPMQSAIEAFKVYLRRNKTLSENVK
                     LLYNNFLKYTDKLSRLTKRDKPKLTELKQKIEETKQIADLGWLQQKVDEKLIDNKR"
     gene            complement(930..2945)
                     /locus_tag="PLB46_11700"
     CDS             complement(930..2945)
                     /locus_tag="PLB46_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF77230.1"
                     /translation="MKKIFILSSLVISALIIEAQAPTQPNFVLIIADDLNDYVQGFDG
                     HPQAKTPNIAKIAKKGTTFTNAYCAGPKCAPSRTSLITGKDAYYTKVYNNDDIGCKNF
                     RKNFTEAKGNATVFTMPELLKDSGGYYTYMLSKVMHCYVGLPDYDTSNVPACEKDLSW
                     SKAFSYGQGGDMDPILDYGGANDDGVNGFFFTPIPDSLETQMVDYIITDSAIAFIEDY
                     ATNPENYCNKPFFLTFGYKRPHGPFYVPEKYFLPYYNTDYYQDPYNKPYNDPVGATPF
                     NGLVMPPQPDPMWSDYDALPDDLVGKVLNEEDYVHPDFLQFGDIMMAEVPPPPIGVGL
                     SDEEKTFILQESERANGIIAYLASIKFMDAQIGRFYNEIKSHPEIFNNTVFIITSDHG
                     YSLGEKTHWRKGCMWETDVRIPLVIAAMRTPAKRTTKRVVSNIDIMPTVLELAGVTLP
                     TFPDGSPYLDGKSLTPILANPNVIWDRPVVSSLQLHGYDEGFCFPQYSVRDDEFHYIR
                     YQTNGAVAPACNEALSVFQEELYRIGKNRNVDPYEWDNLADDPAYNATKEHLATFLPG
                     GVNYLQFDRLTADDATTMDENSQLGVFPNPTSEYANFRIFDITPGEATFTISDITGRI
                     LTSDIIQTDENGYLEFSYNISMLEQGYYFGQLKQQDKTFTVEFIVAR"
     gene            complement(3031..>4180)
                     /locus_tag="PLB46_11705"
     CDS             complement(3031..>4180)
                     /locus_tag="PLB46_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77231.1"
                     /translation="ATIVRILQKIALPFLDAIIILGGMYLVKEYWEYYVKYIEGGEYP
                     INYLLINIPLYIVIWILAIFVGGGYDRSTNISRIVRGMFWGTIVIAAVYGFLPEAYRF
                     SRGMIIAGAAFNTALLIAVRSTLHFMRYKNFRFGEAPEKKILIVGNGEETARAHQLLN
                     QLQLGNLIMGYVRTGNYKNNDELELGDITNLPELINVYKANEIIFCAKDISSQDIISQ
                     MVSLGELIDFKIIPDQSLSIIGSNSKNSAGDIYTVDIQLKITQPESKRAKRLFDIGIS
                     FLVLICAPVLLFVVRNKSGFLRNIFAVFANSKSWVGYFRENGKQLPGGTTPILPKLKP
                     GVLVPVNGHNKGMDNQLMATRLNFIYARDYRVTDDLEIVLTNMRNLGN"
BASE COUNT         1359 a          761 c          717 g         1343 t
ORIGIN      
        1 ttactaaaag aaatttcaga ccatctcaag gaaaatagtt atgaggagta tccatccatc
       61 accatctatt accaaatttt aatgaccttc actgacacgg atacagaggt gcattttgaa
      121 gagctaaaaa aattgttaga tgaacacacc actaaattcc ctcctgacga agctcgagat
      181 atgtatgcgt atgctcaaaa ttttgccata cgtaaaataa atgcagggga aaaacgattt
      241 ttaagagagt attttgattt gtcgaaggcg gcgttggaga aagaattact tatggttgat
      301 ggtgatttat caccttggac attcaaaaat ttagttatag cggcattgcg tgttggtgaa
      361 tttaattggg cagaacaatt tattaaaaat tataaaaatc gcctgaatga aaaattcagg
      421 tcgaatgcct ataattataa tcagggtgca ttattgtttt tcaaagggca atatggtgat
      481 gctttacgat taattaatca ggtagaatat accgacattt tttatgcact ggatacccgc
      541 accatgcaaa ttaaaatata ttatcaactc gatgaatggg acccaatgca aagtgccatt
      601 gaggcattta aagtgtattt gcgaagaaat aaaacattaa gcgaaaatgt aaaattgttg
      661 tataacaact ttttaaaata caccgataaa ttatcaagat taacaaaacg cgataagcca
      721 aagctgaccg agttgaaaca aaaaatagaa gagacgaaac aaatagccga tttaggttgg
      781 ttgcagcaaa aagtagatga aaaacttatt gataataaac gctaacataa gttagtgatg
      841 ttgtgccctt gagttagcaa taaattaata ataaataaaa aagccttgtt acaatattgc
      901 aacaaggctt ttttaatgat tgtaagttgt tatctggcaa caataaattc tactgtaaat
      961 gttttatcct gttgttttaa ctgtccgaaa taataaccct gctctaacat actaatattg
     1021 tatgaaaatt caaggtaacc attttcatct gtttgtataa tatcgctggt taaaattcta
     1081 ccggtaatat cgctaatggt aaaagtagct tcacctggtg tgatatcaaa tattctgaaa
     1141 ttagcatatt cgctcgttgg attggggaat acacctaatt gtgaattttc atccattgtc
     1201 gttgcgtcat ccgcagtcaa tctgtcaaat tgtaaataat ttacaccacc gggtaaaaat
     1261 gttgctaagt gttcttttgt agcattataa gctggatcat cagctaaatt gtcccattcg
     1321 taagggtcaa catttctatt ttttcctatg cgatataatt cttcctgaaa cacgcttaat
     1381 gcttcattac aagccggagc aacagcacca tttgtttgat aacgaatgta gtgaaactca
     1441 tcatcccgaa cactatattg aggaaaacaa aaaccttcat cataaccatg taattgtaat
     1501 gacgatacaa ccggtctgtc ccaaattaca tttggattag ctaaaatagg tgtcaggctt
     1561 ttaccatcta aatatgggga accatcgggg aaagttggaa gcgtaacgcc agccaattct
     1621 aacactgttg gcataatatc tatattactt actactcgtt tagtagttct tttagccggt
     1681 gtgcgcatag cggcaattac caatggaata cgtacatctg tttcccacat acatcccttg
     1741 cgccaatgtg ttttttctcc aagtgaatag ccgtggtcgc tggtaataat aaatacagta
     1801 ttattaaata tttcgggatg cgatttaatt tcattgtaaa atcttcctat ttgtgcatcc
     1861 ataaatttaa tagatgctaa ataggctata ataccatttg ctctctctga ttcctgaagg
     1921 atgaatgttt tttcttcatc tgataatcca actccaattg gtgggggtgg aacttcagcc
     1981 atcatgatat caccaaattg taaaaagtcg ggatgcacat aatcttcttc atttaaaact
     2041 ttaccaacta aatcatcggg taatgcatca taatcgctcc acattgggtc tggttgaggt
     2101 ggcataacaa gaccattgaa aggtgttgca ccaacggggt cgttgtaggg tttattataa
     2161 gggtcttggt aataatcagt attgtaatag ggtaaaaaat atttttcagg tacataaaat
     2221 ggtccatgcg gacgcttata accaaacgtc aggaaaaaag gtttgttgca ataattttcc
     2281 ggattagttg cataatcttc aataaatgca attgcagaat cggtgataat ataatccacc
     2341 atctgtgttt ccagagagtc gggaattgga gtaaagaaaa atccgtttac accatcatca
     2401 tttgcaccac cataatctaa tatcggatcc atatcgcctc cttgtccata tgaaaaagct
     2461 ttactccatg ataaatcttt ttcgcaagca ggaacatttg atgtatcgta atcaggaaga
     2521 ccaacataac aatgcattac tttgctcagc atataggtgt aataaccacc actgtctttt
     2581 aataactcag gcatggtaaa cacggttgca tttccttttg cttctgtaaa atttttacgg
     2641 aaatttttac atccaatgtc atcattatta tacaccttgg tatagtaagc atcctttccc
     2701 gtaattaaac ttgttctgct cggcgcacat tttggtcccg cacaataggc attcgtgaaa
     2761 gtggtgccct ttttggcaat tttcgcaata ttgggcgttt ttgcctgagg gtgaccatca
     2821 aaaccttgaa cataatcgtt gaggtcatca gcaattatta atacaaagtt tggttgcgta
     2881 ggtgcctgag cctcaataat taaggcactt ataaccaggc tgctgagtat aaatattttt
     2941 ttcatcgtgg taattaagtg gtatgtgtta tgagtgttaa ttgttgaaat tagttgtttt
     3001 ttggcaaaaa tatatttttt cggaaaaaaa ttaattgccc aaattgcgca tattggtaag
     3061 cacaatctct aagtcgtcgg ttacgcggta gtctcttgcg tatatgaagt ttaaccgggt
     3121 agccattaac tgattgtcca ttcctttatt atgaccatta accggaacca aaacacccgg
     3181 ctttaatttt ggtagtattg gtgttgtacc accgggtaat tgtttcccgt tttcacggaa
     3241 ataacccacc caacttttag agttggcaaa aacagcaaaa atattgcgca aaaatcctga
     3301 tttatttctc acaacaaata acaaaacagg tgcacaaatc aatactaaaa atgatatacc
     3361 tatatcaaat aatctttttg cccttttact ttcaggctgg gtaattttta gttgaatatc
     3421 taccgtataa atatcacctg ccgagttttt tgaattgctg ccaataatac tcaaactttg
     3481 gtctggtata attttaaaat caattagttc acccaggctt accatctgac tgataatatc
     3541 ttggctacta atatctttgg cgcaaaaaat aatttcgttt gccttatata catttattaa
     3601 ttcgggcaaa ttggtaatat cacctaattc tagttcgtcg ttatttttat aattaccggt
     3661 tcttacataa cccataatta aattgcccaa ctgtaattga tttaacaatt gatgagcgcg
     3721 tgctgtttct tctccattac ctacaattaa aatctttttt tcaggtgctt ctccgaacct
     3781 gaaatttttg taccgcataa aatgtagtgt agagcgaact gcaattagta atgctgtatt
     3841 aaaggcagct ccggcaataa tcatccctcg agaaaatcga tacgcttccg gtaaaaatcc
     3901 atatacagca gcaattacga tagtgcccca aaacatgccc cttactatcc ttgaaatatt
     3961 agttgacctg tcgtacccac caccaacaaa tatggctaat atccaaataa cgatataaag
     4021 tggaatattt atcagcaaat aattaatcgg atattcaccg ccttctatat attttacata
     4081 atactcccaa tattccttaa ccaaatacat tcctcctaaa atgataatgg catctaaaaa
     4141 tggaagtgca attttttgta atatcctgac aatggtagca
//
LOCUS       DAORKW010000320         4180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1040_length_4180_cov_4.602909, whole genome shotgun sequence.
ACCESSION   DAORKW010000320 DAORKW010000000
VERSION     DAORKW010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1040_length_4180_cov_4.602909"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3712
                     /locus_tag="PLB46_11710"
     CDS             <1..3712
                     /locus_tag="PLB46_11710"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF77232.1"
                     /translation="VYASGKNRNCQTCPIPDIDHWETAIFDDDIWEYKNGTTEPPSNW
                     NDVSFAAVWATGPGGFGSGDGDDNTTIGGFSNSFYYRKEFEIEDKSKILKTILSMDYD
                     DGFVAYINDVEVARINMTGSPTHSSYANYWHEALMYMGLNPKMIELDADLMSTLLVDG
                     TNVLAVQVHQYGADDATGRTWLHFGIATPDVFFFDNPDWFSNPGTATEIHTDFKIAAN
                     ENIYLYNPSGDVIDSIYTDNLQVNHVRARLPDAGAWCFTDSPTPLLANIGTCYTGYAE
                     PPVITTAAGFYDVSVDVSISGTTVYYTTDGSNPDAAADLYTAPITYTNTGVLKAMQTE
                     AGKLPSPIVTSSYFIDEPISLPVISVIGNPCDLFNEGPDCIAAYDNAEGWFNRNPKIS
                     VAVEYYDTDKVKQFGDNFKFECVGNYSVSLDQKSMRFVNDEDYGTQSDITYNLFERDK
                     PGMEFIHGFRVRNADQDYYGTRIKDITINRIGMPTRCVSTAYQNCVVFINGEYWGMYG
                     AREEMDEYFLRDNFGVNPDAVDLIKTGWGGEEKTMAEAGSDTSFQALLDLILDNDMTD
                     PDVYAQVVEEIDVTNWVDYIATEVFTDQEEWLYILENNIRLFRSYAPDIKWRYMLWDC
                     TNSQFSSNANTLQSSLNNPNNSLYGDMFNALLENETFHDYFINRFADLINYYFTEEKN
                     DAIIDELKAEMEDEIPRQNSRWGTGSYASWNSNVNQLYTFYDNRNTNQRNHIKTYFGL
                     AGQVDVTLEVNPPGAGYVKISTIVPENLPWTGVYFNGVPVTVTAIPNPGFEFVNWSDN
                     PFIDAPDAISFTNNFTSATTFTANFTGSAITNPIVISEINYNPDASFNGGDWIELHNS
                     ADVAVNVSSYQLKEKYFYSDFTIPENTVIPANGYLVLAEDKNMFNLKYPAVTNVVGDY
                     HFSLKNDSDLIILTDMIGDTIIEINYDDNRPWPFTADGYGRTMELADALLNPALPGSW
                     FEGCIGGSPGEAYTLCMENPLVDEINYNSAAFADAGDWFELYNYGTDVVDLSGWFIKD
                     KTGHTFTIPNGTSLGAESYLAFYSDAAKFATAFPDVTNKLGPLGFSFDGNGDVIVIYK
                     ADGVIYQSVGFDDASPYPLAPDGGGMALQIVDVTENINFASNWKESCPDGTPGTVYLN
                     PCQVNIDDVLVGALMVYPNPANQSITIELPELMNTASQVMLYDLNGKNVYTNNFNGVN
                     NLHIPVYQLMPGIYMIKLQNGEITYTGTFVKQ"
BASE COUNT         1315 a          722 c          845 g         1298 t
ORIGIN      
        1 agtatatgcc tcgggtaaaa atcgcaattg tcaaacttgt cccattcctg atattgacca
       61 ctgggaaaca gccatttttg atgatgatat ttgggagtat aaaaatggga ctacagaacc
      121 accatctaat tggaatgatg tttcttttgc tgccgtttgg gctaccggtc ccggaggatt
      181 tggttcagga gatggtgatg ataatactac tattggcggc ttcagcaatt cattttatta
      241 tagaaaagaa tttgaaattg aagataaatc caaaatcctc aaaaccattt taagcatgga
      301 ttatgatgat gggtttgttg cctatattaa tgatgtggag gtggccagaa ttaatatgac
      361 cggttcgcca acacatagtt catacgcaaa ttactggcat gaagcattga tgtatatggg
      421 gcttaatccc aaaatgattg aattggatgc agatttaatg tcgactttgc tggtagatgg
      481 cactaacgtt ttagctgttc aggtgcatca atatggtgct gatgatgcaa caggcagaac
      541 atggttacat tttggcatcg ctacacctga tgtttttttc ttcgataatc ccgattggtt
      601 cagcaatccc ggaactgcaa cagaaattca tactgatttt aaaatagcag caaatgaaaa
      661 catttatttg tataaccctt cgggagatgt tattgacagt atatatactg ataatttgca
      721 ggttaatcat gttagggcac gattacccga tgcaggagcg tggtgtttta cggattcacc
      781 tacaccactt cttgccaata tagggacatg ttacaccgga tatgcagaac ctcctgtaat
      841 taccacagct gccggttttt atgatgtgag tgtagatgta agcatatcag gaactaccgt
      901 ttattataca acagatggca gtaatccgga tgccgctgct gatttatata ccgcaccaat
      961 tacttacacg aatacaggtg tattaaaagc catgcaaact gaagcaggaa aattaccaag
     1021 tccgattgtt acatcaagtt attttattga tgaacccatt agtttgccgg ttataagtgt
     1081 aattggtaat ccttgtgatt tatttaatga aggccctgat tgtatagcag cttatgacaa
     1141 tgcagaagga tggtttaacc gcaatccaaa aatttcggtg gctgttgaat attacgatac
     1201 agataaagtg aaacaatttg gtgataattt taaatttgaa tgtgttggca attattcggt
     1261 tagtctcgac caaaaaagta tgcgttttgt caacgatgaa gattatggga cacaaagtga
     1321 tattacctat aatttatttg aacgagataa acccggaatg gaatttattc acggattcag
     1381 agtgcgtaat gccgaccagg attattatgg aacaaggata aaagatatta cgattaatcg
     1441 cataggtatg cccaccagat gtgtatctac agcatatcaa aattgtgtgg tatttattaa
     1501 tggcgaatat tggggcatgt atggtgcgcg tgaagaaatg gatgaatatt ttttgcgtga
     1561 taattttgga gtaaatcccg acgctgtcga tttaattaaa acagggtggg gtggtgaaga
     1621 aaaaacaatg gccgaagccg gcagtgatac ttcatttcag gcattgttgg atttaattct
     1681 cgataacgac atgaccgacc cggatgtata tgcgcaggtg gtagaagaaa ttgatgtaac
     1741 caattgggta gattatattg caacagaagt atttaccgac caggaagaat ggttgtatat
     1801 tctcgaaaat aatattcgtt tattcaggtc gtacgcgccg gatataaaat ggcgttatat
     1861 gttgtgggat tgcacgaata gtcagtttag ctctaatgct aacactttac aaagtagttt
     1921 gaataatccc aacaacagct tgtatggcga tatgtttaac gccttactcg aaaatgaaac
     1981 atttcacgat tattttatta accgttttgc cgatttaatt aattattatt tcacagaaga
     2041 aaaaaatgat gcaataattg atgaattaaa agcagaaatg gaagatgaaa tacctcgcca
     2101 aaattcacgc tggggcaccg gttcttatgc atcatggaat agtaatgtca atcagttgta
     2161 tacgttttac gataaccgca ataccaatca gcgcaatcat attaaaacgt attttggttt
     2221 agcaggacag gtagatgtta cactcgaagt aaatcctccc ggtgcaggtt atgtaaaaat
     2281 ttcgaccatt gtccctgaaa atttaccttg gacgggcgtt tattttaatg gagttccggt
     2341 aacagtcaca gcaattccca acccgggatt tgagtttgta aactggagtg ataatccatt
     2401 tattgatgca ccggatgcta tatcatttac gaataatttt acatcagcca ccacatttac
     2461 tgcaaatttc acaggctcag caattacgaa tccgattgtg atatctgaaa taaattataa
     2521 tcctgatgct tcatttaatg gtggtgactg gattgaatta cataattcgg cagatgtggc
     2581 agttaatgtg agcagttatc agttaaaaga aaaatatttt tattccgatt ttactattcc
     2641 tgaaaatacg gttattcctg ccaatggata tttagtttta gcggaagata aaaatatgtt
     2701 taatctaaaa tatcctgccg ttaccaatgt tgttggcgat tatcattttt cgctgaaaaa
     2761 cgacagcgat ttaataatat taaccgacat gattggtgat acaattattg aaataaatta
     2821 tgatgataat cgcccatggc catttacagc agatggatat ggccgcacga tggaattagc
     2881 agatgcacta ttaaatcctg cattaccggg ctcatggttc gaaggttgta ttggtggatc
     2941 gccgggagag gcttatactt tatgtatgga aaatcctttg gttgatgaaa tcaattataa
     3001 ttctgcagcc tttgctgatg caggcgattg gtttgaatta tataattatg gcactgatgt
     3061 ggttgattta agtggatggt ttattaaaga caaaaccggt catacattta ctatcccaaa
     3121 tggaacaagt ttaggtgctg aaagttattt ggcattttat tccgatgcag caaaatttgc
     3181 aacggcattt cctgatgtaa ccaataaatt gggtccgctt ggatttagtt ttgatggcaa
     3241 tggagatgta attgtaattt ataaagcaga tggtgtaatt tatcaaagtg taggttttga
     3301 tgatgcatca ccatatccgc ttgcacctga tggcggtgga atggcattac aaattgtgga
     3361 tgtaactgaa aatatcaatt tcgcttccaa ctggaaagaa agctgccctg atggaactcc
     3421 cggcacagtg tatttaaatc cttgtcaggt aaatattgat gatgtattag ttggtgcatt
     3481 aatggtatat cctaatccgg ccaatcaaag tattactatc gaattacctg aattaatgaa
     3541 tactgccagt caggtaatgt tgtatgactt aaatggaaaa aatgtctata caaataattt
     3601 taatggcgta aacaatttac atattcctgt ttatcagtta atgcccggca tttacatgat
     3661 taaacttcaa aatggtgaaa ttacctacac aggtactttt gtaaaacaat aaaataattt
     3721 tcaactatca atcaggttat cacaaaaagg taacctgatt ttttttgccc aaaataatgt
     3781 ttaatctata ccaaacattt tcaaataaac agccgaaacc tgctctgatg aaactccgat
     3841 tcagcagtat cgttccagga ctccgtccga cgcacaccac aaaaaactcc gagctcaatg
     3901 cgggctgttc cctgttgtcg tggagtctcc agactccaga cgccaccccg gtctcctgac
     3961 cgaaagttgt gttttgctat taatcaatcg ttttggttta taagatatta ttagcctccc
     4021 caccaatatt aaagtgtaac tataatgtca cccctgccgg ggttaaatca taatatcgaa
     4081 attatttaaa aaatgcctac tcaacaaaat ctctgattca gcattatcaa tccattactc
     4141 ctgcaacgca caccacaaaa aactccgagc tctctgcggg
//
LOCUS       DAORKW010000321         4166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1047_length_4166_cov_4.886402, whole genome shotgun sequence.
ACCESSION   DAORKW010000321 DAORKW010000000
VERSION     DAORKW010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1047_length_4166_cov_4.886402"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLB46_11715"
     CDS             <1..704
                     /locus_tag="PLB46_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRF77233.1"
                     /translation="SNEYKILSADYSQIELRVIAALSNDQGMIDAFKQGMDIHSATAA
                     KVFGVAINEVTREMRSRAKAVNFGIAYGQTAFGLSQNLGISRGEAKEIIENYNAQFPG
                     VKQLMDTNIEFARKHGYTQTVRGRKRYIKDINSANQTVRNQAERIAVNSPIQGSAADM
                     IKVAMINIHHELNKRKLKSRMLLQVHDELVFDAFIPELDELREIVVDKMVTAIKLNVP
                     IEAEVGVGANWLEAH"
     gene            728..1711
                     /locus_tag="PLB46_11720"
     CDS             728..1711
                     /locus_tag="PLB46_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRF77234.1"
                     /translation="MQKDNIRLQKIVISIAVFLLLVKFIAYIITHSNAVLTDALESIV
                     NVVAGAFGLYSLILASKPKDESHPYGHGKIEFISASIEGSLILIAGLLIIGKSTFNLF
                     FPQTIQHIESGIILVSFAGIINFLMGYVMEKRGKKSNSLTLVAGGKHLQSDGWSTAGL
                     LVGLTLLYFLQYPWIDSAVAIIFGAYICYEGYKILRQSIAGIMDEADTVLLKGVIDTL
                     QQNREGNWIDIHNMRVIKYGANLHFDCHFTVPYYFTVKEGHDELEKIETIIHKHHETS
                     DEWFVHIDACVPPLMCRICKKTDCLQRQASFEKEVIWNLENVIRNRKHGLD"
     gene            complement(1708..2397)
                     /locus_tag="PLB46_11725"
     CDS             complement(1708..2397)
                     /locus_tag="PLB46_11725"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HRF77235.1"
                     /translation="MKLNKNTVLLFTFLVVAAIHLLGILVKQPLLADVTKVLLMPLLL
                     GHVIALPNRKFIGFNLLVIAILFCWAGDVLLMFTEHNELFFLLGLAAFLIGHIFYMLT
                     FNKMADKNSIGKPLQPLFYLLPMLFAASLLIVLFPNLGEMKVPVIAYATVISLMCVAA
                     MRRWQRTDTPSFVTVLTGAVLFIFSDALIAINKFHTPFNAASLLIMITYIAAQYLIIT
                     GLLKHVHPVKN"
     gene            complement(2394..3629)
                     /locus_tag="PLB46_11730"
     CDS             complement(2394..3629)
                     /locus_tag="PLB46_11730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRF77236.1"
                     /translation="MPAAVNYIVYAIPVFFLLIGIELLIDKSKKTGYYRLNDALSNIN
                     AGITEQVSGAFLKAFVVGVYAWIFDHYKVTTIPPTLLNWTILFIGIDFFYYWFHRLAH
                     EINVLWGGHVVHHQSEEYNLSVALRQGAFQKFGSFLFYLPLAIIGFDPVMFIIISQFQ
                     TLYQFWIHTKTIHLLPKPIEYIFNTPSHHRVHHGRNPKYIDKNHGGTLIIWDRMFGTF
                     QKEEEEVVYGITKPLKTWNPLRAQLDFWKDLFADLSKTKKLTDKLKLLFFPPGWFPQE
                     LGGPQSAPEITPDIAVKYNTVIPVKLNYYVLVQFVIILGLASLFLFTYESYDLTTGLI
                     FMALILFSITSIGMILEANRKAYLIEIGRLLFTVVFYVLMVQQNILHDPNYFGLIGLI
                     VFALGSILVFISMRKTFAK"
     gene            3712..>4166
                     /locus_tag="PLB46_11735"
     CDS             3712..>4166
                     /locus_tag="PLB46_11735"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRF77237.1"
                     /translation="MRIIVILFIWLLGCKPSSNQNTVTPKPESVDYFSNRNHGVIGDS
                     AMVVSAHPEASKVGMAILQQGGNAVDAAIAVQFALAVVYPNAGNIGGGGFMVTRMKDG
                     SSNALDFREKAPSTASRDMFINPANGEVNRKLIDDTTLRDGEQSAGVAFT"
BASE COUNT         1411 a          739 c          798 g         1218 t
ORIGIN      
        1 gcagcaacga atacaaaata ttaagtgccg attattctca aatagagtta cgtgttattg
       61 cggctttaag taatgaccag ggaatgattg atgcatttaa acaaggtatg gatattcaca
      121 gtgctactgc agcaaaagta tttggtgttg ccataaatga agtaacacgt gaaatgcgca
      181 gcagagcaaa agcagtaaac tttggaattg cgtatggcca gactgcattt ggtttatctc
      241 aaaatttagg aatatcgaga ggtgaggcca aagaaattat tgaaaattac aacgcgcaat
      301 ttccgggtgt aaaacaatta atggatacca atattgaatt tgcccgtaaa catggttaca
      361 cccaaacagt gcggggcaga aaacggtata ttaaagatat taactctgcc aatcaaactg
      421 ttcgcaatca ggctgaacgt attgcggtaa attcaccaat acaaggttca gctgccgata
      481 tgattaaagt ggcaatgatt aatattcacc atgaactcaa caaacgtaaa ttaaaatcac
      541 gtatgttgct acaagtgcat gatgagttgg tattcgacgc ctttattccg gaattagacg
      601 aattgcgtga aattgttgta gataaaatgg ttaccgccat taaattgaat gtaccaattg
      661 aagcagaggt tggtgttggt gccaattggc tggaagcaca ttaataatta actttaataa
      721 aaatatcgtg caaaaagata atatcagatt acaaaaaata gttatttcaa ttgcggtatt
      781 tttattactg gttaaattta ttgcctatat tataactcat tctaatgccg ttttaaccga
      841 tgcgttagag tcaatcgtaa atgttgttgc cggcgcattt ggcttataca gtttaattct
      901 tgcatctaaa cctaaggatg aaagtcaccc ttacggacat ggtaaaattg aatttatttc
      961 tgccagcata gaagggagtt taatattaat tgcaggttta cttattatag gcaaatcgac
     1021 gtttaattta tttttcccgc aaactattca acatattgaa tccggtatta ttctggtaag
     1081 ttttgccggc attatcaatt ttttgatggg ttatgtgatg gagaaacgcg gcaaaaaatc
     1141 gaattcgctc actttagttg caggaggaaa acacttacaa agcgatggct ggagtacagc
     1201 gggattgtta gttgggctta ccttattgta ttttttacaa tatccctgga ttgacagcgc
     1261 tgtagcgata atttttggcg cttacatttg ttatgaaggt tataaaattt tacgccaaag
     1321 tattgccgga attatggatg aagcagacac tgtactgtta aaaggagtaa ttgatacttt
     1381 acaacaaaac agagagggta attggatcga cattcataac atgcgcgtaa ttaaatatgg
     1441 cgctaattta cattttgatt gccattttac cgtgccttat tatttcaccg ttaaggaagg
     1501 acatgatgag ttggaaaaaa ttgaaacaat tatacataaa caccatgaaa catcggatga
     1561 atggtttgtg catattgacg cctgtgttcc accattaatg tgtcgtattt gtaaaaaaac
     1621 agactgctta caacgacagg cttcttttga gaaagaagta atctggaatt tggaaaacgt
     1681 aattcgcaat cgcaaacacg gattagatta attcttaaca ggatgcacat gtttgagcag
     1741 accggtaatt atcaggtact gagcagcaat ataagttatc attataagca gtgaagccgc
     1801 attaaatggt gtatgaaatt tattaatagc tataagtgca tctgaaaaaa taaacaaaac
     1861 tgctccggtc aaaacagtta caaaactagg tgtatctgtt ctttgccatc tgcgcatagc
     1921 agctacacac atgagggaga ttacggtagc ataggctatc accggaactt tcatctcccc
     1981 taaatttgga aataatacaa taagcagcga cgcagcaaat agcattggaa gcagataaaa
     2041 caatggctga agtggttttc cgatactgtt tttatctgcc attttattaa acgtaagcat
     2101 gtaaaaaata tgtccgatta aaaacgctgc caatcccaac aaaaagaata attcattgtg
     2161 ttctgtaaac attaataaca catcacctgc ccaacaaaat agaatagcta taaccaacag
     2221 attaaacccg ataaacttcc gattaggcaa tgcaattaca tgacctaata ataatggcat
     2281 taacagcact ttcgtaacat ctgccaacaa gggttgttta actaatatac caagtagatg
     2341 aatggcggct acaacaagaa aagtaaaaag caatacggta tttttattca acttcatttt
     2401 gcgaatgttt tacgcatact tatgaatact aatattgaac ccaatgcaaa tacaatcagc
     2461 ccaataagcc caaagtaatt cgggtcatgt aatatatttt gttgcaccat caacacataa
     2521 aaaactacag taaataacaa gcgaccaatt tctatcaggt aggcttttcg atttgcttct
     2581 aaaatcatcc ctatgctggt aatagaaaat aaaatgagtg ccataaaaat taagcctgtt
     2641 gttaaatcat aggactcata ggtgaataaa aacaaggatg ccagtccgag gataataaca
     2701 aactgcacca gcacgtaata attcaatttc accggaataa ccgtattgta tttaactgca
     2761 atatccggcg tgatttccgg agccgattgc ggtcctccca attcttgcgg aaaccatccg
     2821 ggagggaaaa ataatagttt aagtttatca gttagttttt ttgttttaga caaatcggca
     2881 aataaatctt tccaaaaatc caactgtgct cttaaggggt tccaggtttt taaaggtttg
     2941 gtaataccat acactacttc ttcctcttcc ttttgaaatg tgccaaacat tctatcccaa
     3001 ataattaaag tgcctccatg atttttatcg atgtattttg ggtttcgccc atgatgcacc
     3061 ctatgatgag aaggtgtatt aaaaatatat tcaatgggtt ttggcaacag atgaatagtt
     3121 tttgtatgaa tccaaaactg atataaagtt tggaactgac taataataat aaacattacc
     3181 ggatcaaagc caattatcgc aagtggtaaa taaaacaaaa acgagccaaa cttttgaaat
     3241 gctccctggc gcagtgccac actcaaatta tattcttcac tttgatgatg caccacatgg
     3301 ccgccccaca acacattaat ttcatgcgct aagcgatgaa accaataata aaaaaaatct
     3361 atcccaataa ataaaatggt ccaatttaat aatgtaggcg gaattgttgt taccttataa
     3421 tggtcgaaaa tccaggcata aacacctaca acaaatgctt ttaaaaatgc acctgaaacc
     3481 tgttcggtaa ttccggcgtt aatattcgag agggcatcat ttaaccgata gtaacccgtt
     3541 tttttcgatt tatcgatgag caattcaatg ccaattaaaa gaaaaaaaac aggaatggca
     3601 tatacaatat aattaactgc tgctggcata tgctgtcaaa tttatactat ttatttcaaa
     3661 tggtgttcaa atgtaaaaaa gcggtttatg cggatatgct atctttacag tatgcgaata
     3721 atcgtaatcc tttttatatg gctgcttggt tgtaaacctt catccaacca aaacactgta
     3781 actccgaaac cggaatctgt agactatttt tcgaatcgta accatggggt aattggcgac
     3841 agcgccatgg tggtaagtgc gcatcctgag gcatccaaag taggaatggc tatattacaa
     3901 caaggaggaa atgcagttga tgcagctatt gcggttcagt ttgctttggc tgtggtgtat
     3961 cccaatgcgg gcaatattgg tggcggtgga tttatggtta cccgaatgaa agatggcagc
     4021 agcaatgcat tggattttag agaaaaagcg ccttcaacag cttcacgcga tatgtttatt
     4081 aatccggcaa atggcgaagt aaatcgcaaa ctgatcgacg acactaccct ccgtgatggg
     4141 gagcagtcgg cgggagtcgc cttcac
//
LOCUS       DAORKW010000322         4153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1052_length_4153_cov_3.938507, whole genome shotgun sequence.
ACCESSION   DAORKW010000322 DAORKW010000000
VERSION     DAORKW010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1052_length_4153_cov_3.938507"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PLB46_11740"
     CDS             <1..981
                     /locus_tag="PLB46_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF77238.1"
                     /translation="QQNITRYSTFIPNFKNYSGGIYALETYQLNDWSLEAGLRYDYKW
                     TKAFFYDEGELQTPEHRYSALSWNIGSTYFDRNHTTFNINFSSAWRAPAMNELYSDGL
                     HHGAAALEYGDPGLLQERSYQLITGFEYETPKTYIEIGAYNNYMQNFIYLQPVLPPAL
                     TIRGAFPVFEYQQTNANILGIDATAHFHITEQFMVETKFSMLRARDLENDVWVVMMPA
                     DKLSGKVHYIFKNFGKIENLDAGVAVVHVFKQNRTYAEADYLPAPEAYTLTSVSIGSD
                     IICKQHTFSWSIEAENLFNVAYRDYLNRFRYFADDLGRNITLRIKIPFTI"
     gene            998..1555
                     /locus_tag="PLB46_11745"
     CDS             998..1555
                     /locus_tag="PLB46_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77239.1"
                     /translation="MKKQTILFSVLGLLSLQMFLFSSCKEEEIPDVQNEEELITTLIL
                     EFENTISGEVRTFEFRDTDGPGGSEPVDFDTIRLDDNSSYHITVSLLNESVSPADDIT
                     TEVEEEGVDHQLFYVTSGGLNLFISYDDEDANGDPIGLHAMAATTDASTGTTTVTLKH
                     QPDIKDGNILTGDTDVEVIFVTEVE"
     gene            complement(1642..2043)
                     /locus_tag="PLB46_11750"
     CDS             complement(1642..2043)
                     /locus_tag="PLB46_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF77240.1"
                     /translation="MSFLAKQLRVARPTQQLEKVIEFYTKGLGLKIIGEFKNHAGYDG
                     VMIGDEHLSYHLEFTVHAAHTQTNPPGNENLLVFYFATETEWQLTQEQISNLGYAPVA
                     PENPYWLNNSVTFEDPDGWRIVLYRGLYEIK"
     gene            complement(2055..3449)
                     /locus_tag="PLB46_11755"
     CDS             complement(2055..3449)
                     /locus_tag="PLB46_11755"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRF77241.1"
                     /translation="MKLKFCGAAGTVTGSSHLLTLDNGTKILLDCGLYQGAEVGYENF
                     NEKFLFNPAEIDIVVLSHAHIDHCGRLPKLVKDGFRGRIYATHATRDLTAILLLDSAH
                     IQERESAFTNKRRKEDNEDEPLYSPEDVALTMELFRTVGYNQWFGIHPDVDVVFKDSG
                     HILGSASVTMKIRRQGLKDFIFGFTADIGRPNRPILRDPEPMPACDFLICESTYGDRL
                     HEDSPEEKNHFLHIIKQTCVEQKGKLIIPAFSVGRTQEIVYMLDQLVKENLLPNVPVY
                     VDSPLAVNATRVFEMHPECFDKDILAYMQHDANPFGFDNLRYTRSVDESKSINAKKGC
                     IVISAAGMINAGRVKHHVYNAIENPANTILIVGYCADNTPGGQLRNGAETIRLFGEYK
                     QVNAKVEIMSSFSAHGDYREMIDYLRTQEKSKLRKMMLVHGDKNALTGFKEHLEASGY
                     QNVFIPRLGDTVDL"
     gene            complement(3587..>4153)
                     /locus_tag="PLB46_11760"
     CDS             complement(3587..>4153)
                     /locus_tag="PLB46_11760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HRF77242.1"
                     /translation="KGVGATLIAGSNFSAIGAIVRIKANIHGETKWQMRELSAQNSFN
                     SMLSLQAEFGLGDAASIDSLIVEWPSGIIDYCGSLSANHFYTMFEGACPEDIVSINNT
                     TNNISFSLSHNPVSDRLTVLFEVSEQTPVTISITDSAGKNVVHQSYANLPSGENMINL
                     QLNNLAAGVYYCHIVQGQQHATKLFTKQ"
BASE COUNT         1302 a          770 c          752 g         1329 t
ORIGIN      
        1 caacaaaaca ttacgcgtta tagtacattt attcccaatt tcaaaaatta ttccggtggt
       61 atttatgcat tagaaaccta tcaattaaat gactggtcgc ttgaagcagg tttacgatat
      121 gattataaat ggacaaaagc gtttttttat gatgaaggtg aattacaaac accggaacat
      181 cggtacagtg cattaagctg gaatattggc agcacctatt tcgatagaaa tcacaccacg
      241 tttaacatca atttcagttc ggcatggcgc gcacctgcca tgaatgaact ttatagtgac
      301 ggcctgcatc acggtgctgc agcattggaa tatggcgacc caggtctatt acaagaaagg
      361 tcgtatcaat tgattaccgg atttgaatat gaaacgccaa aaacttatat tgagataggt
      421 gcttataata attatatgca aaatttcata tatctgcaac cagtattacc tcctgcttta
      481 actatacgag gtgcatttcc tgtatttgaa taccaacaaa ctaatgccaa tattttggga
      541 attgatgcaa ctgcacattt tcatattaca gagcaattta tggtagaaac aaaattttcg
      601 atgttgcgtg cccgcgattt agaaaatgat gtttgggtag taatgatgcc tgcggataaa
      661 ttaagtggta aagtgcatta catttttaaa aattttggca aaatcgaaaa tttagatgca
      721 ggtgttgctg ttgtgcatgt atttaaacaa aatcgcactt atgctgaagc tgattatttg
      781 ccggctcctg aggcgtatac gttaacaagt gtatcgattg gcagtgatat tatttgtaaa
      841 caacatacct tttcctggag cattgaagca gaaaatttat ttaatgtagc ataccgcgat
      901 tatttaaatc gtttccgata ttttgccgat gaccttggca ggaatattac tttaagaatt
      961 aaaattccat tcacaattta aaaaaaaatt aatgaatatg aaaaagcaaa ctattttatt
     1021 tagtgtatta ggcttgttaa gccttcagat gtttttattt tcaagctgca aagaggaaga
     1081 aattccggat gtgcaaaatg aagaagaatt aattaccact ttaattttgg aatttgaaaa
     1141 taccatttca ggtgaagtgc gcacatttga attccgcgat actgatggac ccggaggtag
     1201 tgaacctgta gactttgata ctattcgttt agacgacaac tcaagttatc atataacagt
     1261 ttcactgttg aatgaaagtg taagtcctgc ggatgatatt actacagagg tagaagaaga
     1321 aggtgttgat catcaattat tttatgttac atcaggtgga ttgaatttat ttatcagcta
     1381 tgatgatgaa gatgcaaatg gcgatcctat cggattacat gccatggctg caactaccga
     1441 tgccagcaca ggtacaacaa ctgttacctt aaaacatcag ccggatatta aagacggcaa
     1501 tatcttaaca ggggatactg atgtggaagt aatttttgtt actgaagttg aataacactt
     1561 acttattgta ttttttacaa tatgtaccaa aagctgaatg aataggggtt aatatatagc
     1621 gtgatccgta aacggattgc gctattttat ttcatataat ccgcgataaa gaacaatacg
     1681 ccacccatca gggtcttcga atgttacact gttgttgagc caataaggat tttccggagc
     1741 aacaggggca tatcccaaat tactaatttg ttcctgtgtt agttgccatt cagtttcagt
     1801 agcaaaataa aaaactaata aattttcatt tccgggtgga ttagtctgcg tgtgtgcagc
     1861 atgaacagtg aattccaaat gataggataa atgttcatct ccgatcatca caccatcata
     1921 accagcatga tttttaaatt caccaattat tttaagccct aaacccttgg tgtaaaattc
     1981 aattactttt tccaattgtt gagtaggtcg agcaacacgt aattgtttag ctagaaatga
     2041 catgtttaat gttattataa atcaaccgta tcacctaaac ggggaataaa tacattttga
     2101 tatcctgagg cttcgaggtg ttctttaaat cctgttaatg catttttatc gccatgtacg
     2161 agcatcattt tacgcaattt tgatttttcc tgtgtgcgta aataatcaat catttcacga
     2221 taatcgccat gtgcagaaaa tgaactcata atttccactt tggcatttac ctgtttgtat
     2281 tcgccaaaca atctgatagt ttctgcacca ttgcgcaatt ggcctccggg tgtattatcg
     2341 gcacaataac ctacaattaa aatggtattt gccggatttt caatagcatt ataaacatgg
     2401 tgtttaacac gacctgcatt aatcatacct gcagcgctga ttacaataca tccttttttt
     2461 gcattgatac ttttcgattc atccacggat cgcgtgtatc tcaaattatc gaaaccaaat
     2521 gggttagcat catgttgcat gtaggctaaa atatctttat caaaacattc aggatgcatt
     2581 tcgaaaacgc gagttgcatt cactgccagc ggtgaatcca catacaccgg aacattgggt
     2641 aataaatttt cttttaccaa ttgatccagc atgtaaacaa tttcctgtgt acgaccaaca
     2701 ctaaatgctg ggataattaa ttttcctttt tgttcaacac aggtttgttt aatgatatgt
     2761 aaaaaatgat ttttttcttc cggtgaatct tcatgtaatc tgtcgccata agtagattca
     2821 caaattaaaa aatcgcaagc aggcattggt tccgggtcgc gcaaaattgg gcgattaggg
     2881 cgaccgatat ctgcagtaaa tccaaaaata aaatctttca aaccttgtct gcgaattttc
     2941 atcgtaacac tggcgctacc taaaatatgt cctgaatctt taaataccac atcaacatca
     3001 ggatgaatac cgaaccactg gttatatcca acggtgcgaa ataattccat ggttaaggct
     3061 acatcttccg gagaatacaa aggttcatct tcattatctt cctttctgcg tttattggta
     3121 aatgcacttt cacgttcctg aatatgtgcg ctgtcgagta gtaagatggc agttaaatca
     3181 cgcgttgcat gtgtagcata aattctaccc ctgaaaccat ctttcactaa tttgggtaaa
     3241 cgaccacaat ggtcgatatg tgcatgcgat aaaactacga tatcaatttc tgcaggatta
     3301 aataaaaatt tctcattaaa attttcgtat cctacttcgg ccccctgata taaaccacaa
     3361 tccaataata ttttagtccc attgtcgagg gttaataaat gagaactacc cgtaacagtg
     3421 ccagccgcac cacaaaattt taatttcata cctaagtctt tagcgcaact aaggtaatat
     3481 tattttgagg atatcataca tgttgttttt cacctgttgt gatgggctta aaaaaacgag
     3541 gcccgttgtt attcacaatc gggcccctgc ttaccatcct tacggattat tgttttgtga
     3601 acagtttagt agcatgctgt tgcccttgaa caatatggca ataatatact ccggcagcaa
     3661 ggttgttgag ttgtaagttt atcatgtttt cgccggatgg taaattcgca taggattgat
     3721 gtacaacatt tttacctgca ctgtctgtaa tactaatagt tacaggcgtt tgctcactca
     3781 cctcaaataa tactgttaac cggtctgata ccggattatg cgaaagtgaa aaggaaatat
     3841 tatttgtcgt attatttatg ctgacaatat cttccgggca cgcaccttca aacatagtgt
     3901 aaaaatgatt cgcagataaa cttccacaat aatcgattat accggaaggc cactcaacaa
     3961 ttaatgaatc tattgatgcg gcatctccta atccaaattc ggcttgcagt gacagcatac
     4021 tattaaatga attttgtgct gataattcac gcatctgcca ttttgtttca ccatggatgt
     4081 ttgctttaat tcttactatt gctcctattg cagaaaaatt agagccggca attaaagttg
     4141 caccaactcc ttt
//
LOCUS       DAORKW010000323         4140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1055_length_4140_cov_3.131212, whole genome shotgun sequence.
ACCESSION   DAORKW010000323 DAORKW010000000
VERSION     DAORKW010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1055_length_4140_cov_3.131212"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PLB46_11765"
     CDS             complement(<1..702)
                     /locus_tag="PLB46_11765"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRF77243.1"
                     /translation="MIKHLFTVYLVLIITGLHSQSINISNGELFDGEPFMVVNSSNTS
                     HIVIAWMGFDGLSLITMKLKISEDGGATWSAPITLPHINPGYTCADPSMGYDNAGNLF
                     FSYIDYNPFGTSGKAIVRKSTDGGYTWGDPVEVMDVFSDPGEAAVDRPWMVIDKSGTS
                     TDGNIYITTKPAPWIPFPNRNYITASTNNGASFNPWQYIDGPGGSIGDFIAAPMATPA
                     VGIDGTFYCVYPSWDP"
     gene            complement(746..1159)
                     /locus_tag="PLB46_11770"
     CDS             complement(746..1159)
                     /locus_tag="PLB46_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77244.1"
                     /translation="MELTHYFQPGEIAPGEGAWVFLMRSPISPDESIAEQITQFYSNA
                     YMAICNDELAHMYGFDSKHDLVGAPVSTFMPSSNSILELLETMIKSDYKLSDAISKEH
                     DKDGRPIFFRNSMEAIFKDGLLIGGKGTQILIEKP"
     gene            complement(1386..2714)
                     /locus_tag="PLB46_11775"
     CDS             complement(1386..2714)
                     /locus_tag="PLB46_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRF77245.1"
                     /translation="MQTNITYREIIRLSTPLMISSLFNNLISLIGVAFMGRVGETELA
                     ASGVAALIYITLTMIPYGLSVGFQIIIARRAGENNPESIGRIFNANLIFLMGVAFLLY
                     AFLEYVTPAILKPRFSPEVFAAVMDYLQYRNFEIFFAVFAFTILGFYTSIGNNAIITW
                     SAILMLIVNVFFNYNLVFGEMGFPEMGIAGAGLASLISSTAACGLNFFYLLFSKYRHQ
                     FGLFKFHKFEWDLVKSGLVISGPVVLQHLLSTGTWVIFFMMVSKMGPASLAASNVAKE
                     VYMVLGVTTWGIANATNSLVSNLLGQNRHSEIFVLLKRVIIVALGFSLIFFAFIMIWP
                     QVFIRIFTNDEAIVALSINPVRIVGICLMMMAVSSVLFRAVTGTGATRYSLLLELITL
                     VVYMVYVVIIIEVLNVSLAVAWTSEFVYWLVLAWLCWKYLKSGKWKKYKV"
     gene            complement(2846..3814)
                     /gene="hemB"
                     /locus_tag="PLB46_11780"
     CDS             complement(2846..3814)
                     /gene="hemB"
                     /locus_tag="PLB46_11780"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRF77246.1"
                     /translation="MQIRPRRLRNSGLMREMVAETRLSKEMFVYPVFVMKGNAIKHEI
                     GAMPGIYHFSPDTLCEEVRECLALGISRFLIFGVGETKDEVGSSCFDHANAVATAVRA
                     LRADFGNSIMIITDVCMCAYTSHGHCGILHGHEVANDDTLESLAKMALMHAEAGADVV
                     APSDMMDGRVGAIRQILDENGYSNTAIMSYSVKFASGYYGPFREAADSAPSFGDRKTY
                     QMDFRNGREALKEALLDVDEGADMLMVKPALAYLDIIKSVKEHTLLPLACYNVSGEYS
                     MVKAAAQKGWIDEQRIVMENMMAFVRAGADIIITYHAKDILKNGWM"
     gene            3899..>4140
                     /locus_tag="PLB46_11785"
     CDS             3899..>4140
                     /locus_tag="PLB46_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HRF77247.1"
                     /translation="MLHFIDKDFNKDFTRHYSLLFQLEEQQYAYAVYEKRTGKLQVLK
                     SIQLNSASSGDILTNLKNSVTADDILQAPYHEAKVG"
BASE COUNT         1282 a          916 c          782 g         1160 t
ORIGIN      
        1 cggatcccaa gatgggtaaa cgcaataaaa agtgccatct attcccactg ccggagttgc
       61 catgggagcg gcaatgaagt ctccgataga accgccgggg ccgtcaatgt actgccatgg
      121 gttaaatgaa gcaccattat ttgtggaggc tgtaatataa ttgcggtttg gaaacggaat
      181 ccaaggggca ggttttgtgg tgatgtaaat attaccatca gttgatgttc ccgatttgtc
      241 gataaccatc cacggcctgt cgactgctgc ttcacccggg tcagaaaata catccattac
      301 ttctaccggg tcgccccatg tatatccgcc gtcggtcgat tttcggacta tcgctttgcc
      361 ggacgtacca aacggattgt aatcgatata actgaaaaag aggttgcctg cattatcgta
      421 acccatcgat gggtctgcac aggtatatcc gggattgata tggggtaggg taatgggagc
      481 actccaggta gcaccaccat cttcactaat cttgagtttc atggtaataa ggctaagtcc
      541 atcgaacccc atccaggcaa taacaatatg tgatgtattg gatgagttaa ctaccataaa
      601 tggttcacca tcaaatagtt ctccatttga aatattgata ctctgactgt gtaacccggt
      661 tataatcaaa accagataaa cagtaaatag gtgcttaatc atacttttgt ttagaccagt
      721 ccgaacttga gaggtttaaa caatattacg gtttttcaat cagtatttgt gtccctttac
      781 ctccaattaa caacccatcc ttaaaaatgg cttccatact gttgcggaaa aaaattggcc
      841 ttccgtcctt atcatgctcc ttgcttatag catcagaaag tttataatca ctctttatca
      901 ttgtttcgag caattccaga atactgttgg atgacggcat aaatgtagaa actggagcgc
      961 caaccaaatc gtgtttgctg tcaaaaccat acatgtgagc caactcatcg ttgcaaattg
     1021 ccatataagc gttgctgtaa aattgagtta tttgttcggc aatggactca tccggtgaga
     1081 ttggtgagcg cattaaaaat acccatgctc cttcgccggg agcaatttct cccggttgga
     1141 aatagtgtgt aagttccata aactgtcgtg ttggtgttgt ctggaaatca attagttaca
     1201 atcaatttca aagtgaaagt tactcatttt tttgttaagc acagcatgac ttaccttctt
     1261 tttgtaactg attttatttt caaaaagtta caatttatat caatttacaa tacaataaat
     1321 ttgatataaa caattgattt acaataaaat acaatattta actaatgtag tgaagtagtt
     1381 aacggttaaa ccttatactt tttccattta ccacttttaa ggtatttcca acataaccat
     1441 gcaagtacca gccagtagac gaattcgctg gtccaggcta ccgcgaggct aacattcagt
     1501 acttcaataa tgatgacaac gtaaaccata taaaccacta atgtgatcaa ttctaataat
     1561 aaggaatatc tggtagcacc cgttccggta actgctcgga ataacacact tgaaactgcc
     1621 atcatcatca aacaaatacc aactattcgg acagggttaa tagacagggc cacaatagct
     1681 tcgtcgttag taaatatgcg aataaacacc tgaggccaga tcataatgaa ggcaaaaaat
     1741 atcagtgaaa accctagagc tacaattata acgcgcttta atagcacaaa aatctcagaa
     1801 tgcctgtttt gtcccagaag attactcacc agactgtttg ttgcgttggc aattccccag
     1861 gtggttacgc ctaacaccat atatacttct ttagccacat tggaagccgc taaagatgcc
     1921 ggtcccattt tactgaccat cataaagaaa atgacccagg tgcctgtaga tagcaagtgt
     1981 tgcaaaacaa cgggaccact gataacgagt ccgcttttca ctaaatccca ctcaaacttg
     2041 tggaatttga ataggccgaa ctgatggcga tatttggaaa acagtaaata gaagaagttc
     2101 aaaccacaag cggccgttga tgatatgagc gatgccaatc ccgccccggc aatacccatt
     2161 tcaggaaaac ccatctcccc gaaaacaagg ttataattaa aaaacacatt gacaatcagc
     2221 atcaaaatag cgctccaggt aataatggca ttgttgccaa tactggtata aaaccctaaa
     2281 atggtgaaag caaataccgc aaaaaatatc tcgaagttcc ggtattgaag atagtccatc
     2341 accgctgcaa acacctcagg gctaaacctg ggttttaaaa ttgccggggt aacatactcc
     2401 aaaaaggcat acaacaagaa ggcaacaccc attaaaaaaa tcaggttggc attaaatata
     2461 cgtcctatgg attcggggtt attttcacct gcccgccgag ctataattat ttgaaaacca
     2521 acggataagc cgtatggaat catggtcaat gtgatgtaga ttaacgcagc cacacctgat
     2581 gctgccagct ccgtttcacc aactcttccc ataaatgcta caccaatcag gctgatcagg
     2641 ttgttgaaca aactactaat catcagcggt gtgctgagcc tgataatttc tctgtatgtg
     2701 atattggttt gcaaggctgc ttaagcggaa ttgtcggatt gaagcgtaaa gatgtaaaag
     2761 agtttgataa gaatgtaacc ctacaggtta acaaaagttg aatatatctg aaaaattata
     2821 cactatactc caacattctt tcgctttaca tccatccgtt tttcagtata tccttcgcat
     2881 ggtaggtgat gatgatatcg gctccggctc taacaaaggc catcatattt tccatcacaa
     2941 ttcgttgttc atctatccat cccttttggg cagcggcttt caccatactg tattcacctg
     3001 aaacattgta acaggctaat ggtaacaagg tatgttcctt tacagactta ataatatcca
     3061 aataagctaa ggccggtttt accatcagca tgtcggcgcc ctcatctaca tctaataaag
     3121 cttctttgag cgcttcacga ccattcctga agtccatttg gtatgttttt ctgtccccaa
     3181 acgaaggagc gctatcggca gcttcacgaa aagggccata atagccggat gcaaatttca
     3241 ccgaataact catgatggcc gtattgctat atccgttttc atctaaaatc tggcgaattg
     3301 caccaaccct gccgtccatc atatcacttg gagccacaac atccgcacct gcttcagcat
     3361 gcatcaatgc cattttagcc agggattcca aagtatcatc gttcgcaacc tcgtgtccgt
     3421 gtaaaatgcc acaatgtccg tgactggtat aagcgcacat acacacatcg gtgataatca
     3481 taatgctgtt accaaaatcg gctctcagcg cccttacagc tgttgcaacg gcattggcat
     3541 ggtcgaagca ggaagaaccc acttcatctt ttgtttcacc tacaccaaaa atcaaaaatc
     3601 gggatatccc taatgctaaa cactctctta cttcttcaca taacgtgtcc ggcgaaaaat
     3661 gataaatgcc gggcatggca ccaatttcat gtttaatagc attgcctttc atcacaaaca
     3721 ccggataaac aaacatttct ttacttaaac gggtttcggc taccatttct cgcatcaatc
     3781 cactgttgcg caatcttctc gggcgtattt gcattgctga ttatttttgc tgtaaagtta
     3841 ctaccacaaa cggggaaaat taattccgtt aaaaaaaaca agtattttta ctgcgaatat
     3901 gctgcatttt atagataaag attttaataa ggattttacc aggcattatt ctttgttgtt
     3961 tcaactcgaa gaacagcagt atgcttatgc ggtttatgaa aaacgaacag ggaaactgca
     4021 ggtgttaaaa agtatacaat tgaatagtgc aagttccggc gatatattaa ccaacctcaa
     4081 aaattcggta acggcagatg atattttgca ggcgccttac cacgaagcta aagtcggctt
//
LOCUS       DAORKW010000324         4131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1062_length_4131_cov_4.907507, whole genome shotgun sequence.
ACCESSION   DAORKW010000324 DAORKW010000000
VERSION     DAORKW010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1062_length_4131_cov_4.907507"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..834)
                     /locus_tag="PLB46_11790"
     CDS             complement(148..834)
                     /locus_tag="PLB46_11790"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF77248.1"
                     /translation="MKTYLIIGGSSGIGAAVVDELAHENNVMATYCKNYKKDNINVSF
                     HELDVFNENPITFIPEKLDGLVYCPGSILLKPFARINGADFLKDYQLQVVGATKIIQS
                     ALPALKNSEQASVVLYSTIAVQRGFTFHSLVSASKGAIEGLTRALAAEFAPKIRFNCI
                     APSITNTPLASGLLNTPEKMEANAQRHPLKRIGTVSDIAKMTAFLLSENSSWMTGEIL
                     HVDGGMSSIK"
     gene            complement(980..1288)
                     /locus_tag="PLB46_11795"
     CDS             complement(980..1288)
                     /locus_tag="PLB46_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77249.1"
                     /translation="MQIVFATFLSLILMTSTPKDYASFTLRNNSAKSIPLNIPSVMNP
                     NLSPFSNSGVSLKIGQEIFFYENKKKYLLLMVTPDLEGDTLYVNDLIKTRKTELNIEK
                     "
     gene            complement(1491..3158)
                     /locus_tag="PLB46_11800"
     CDS             complement(1491..3158)
                     /locus_tag="PLB46_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77250.1"
                     /translation="MNSKHLLTILMLFTATILAAQQIPDQEDVGDLINVPCPYDPVHF
                     VDLATGWIAYQTTDNTSFGPVDTTLCISTKADPDYFGISFDIDEMDTSKPVFLKAVFN
                     DTNKIKLDSNFVYHLSLNGINFSDFEDGADTVEYYKSAIILGLEHPDSTDTTYVIDQQ
                     YIDLNSSNYMSEICFASSKFEVQYISSFIYKFNLAMDVDDIIYFYPPYVQPVYETNII
                     EDFIGGGSFGADDHYKIYIEGYFGGPRYYLGVYNKPNYPSAETIDTLLAMPEPNQPES
                     TVIDIEFTGWSTFIQQNYTLIAGGMPLEDTIPHSVNVILNDGMFCEAYDIIYGNRVAF
                     IYRGGMIDLPTPGSCFNFYPGSTFIVDDNAKLEYGLSGSGMLWLHSGSTIELRKNAKL
                     IIGNTVKISDASDKFNNVYITLDQGDSLIFQNGSHIKLDTATENMFICLYMKGGYADI
                     SGLPLSEQKYIRFIYPNPKPEPINLLTLYPNPFEEYVVVEFAMEISEQVTIKIYNLKG
                     EQVYSQQESTLAGINSFAIPLQSLHSGNYLIEISNGNRKVVQNLVKL"
     assembly_gap    3688..3787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3858..>4131)
                     /locus_tag="PLB46_11805"
     CDS             complement(3858..>4131)
                     /locus_tag="PLB46_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77251.1"
                     /translation="LVLVKGRKGEEVTELIEVADFIDIKGWKSQGNRLSQYEIKKVTG
                     KKGETTDDETTSQPETKKSNVKVGTQMEWDLEDKKGKDKGEQTKLF"
BASE COUNT         1199 a          771 c          724 g         1337 t
ORIGIN      
        1 tgattagtat tatcggttaa aatgtgaaaa cccatatgat caggggttat cactttcaaa
       61 ttatctgcag aagacaaaaa gtcccaggct tcttgtaaag agataggaat ttgttgtttt
      121 gttttaattg tatatacacc cataatatta ttttatactc gacatgccac cgtccacatg
      181 taaaatttct ccggtcatcc agctgctgtt ttcagacaac aaaaatgcag tcatttttgc
      241 aatatctgat actgttccaa ttctttttaa aggatgccgt tgtgcgttag cttccatttt
      301 ttccggtgtg tttaataaac ccgatgctaa tggtgtgttg gtgatggaag gtgcaataca
      361 attgaatcgt attttaggtg caaattctgc cgctaatgct ctagttaatc cttcaatagc
      421 tccttttgat gcggaaacca gtgaatgaaa ggtaaacccg cgctgcacgg caattgtaga
      481 gtataaaact actgaggcct gttcactgtt ttttagcgcc ggtaaagcag actgaataat
      541 ttttgttgcg ccaacaacct gcaactgata atcttttaaa aaatcggctc cgttaattct
      601 cgcaaatggt tttaataaaa tacttcccgg acaataaacc aacccatcaa gtttttccgg
      661 aataaatgtt attggatttt cattgaagac atctaattca tgaaaactta catttatatt
      721 atccttttta taatttttac aataagtagc cattacatta ttttcatgtg caagttcatc
      781 caccacagca gcgccaattc cggatgaacc ccctatgatt aaatatgttt tcatttaagt
      841 gataattttt tatttctttt acatttttcg ctgcaataaa tgacttcatt ccaatccttt
      901 tcccactttt ttctccaagt aaattcacgc ttacacacag ggcaggtttt ttgggggaga
      961 tgtaattttt tgtgcactgt tacttttcga tatttaattc agtcttacgt gtttttatta
     1021 agtcatttac ataaagtgta tcgccttcca agtcgggtgt aaccattagt aataaatact
     1081 tctttttatt ttcataaaag aatatttcct gtccgatttt taatgaaaca ccactattag
     1141 aaaatgggga aaggttgggg ttcattacag atggaatatt gagtggaatt gattttgcgc
     1201 tattgttgcg aagggtaaac gaggcgtaat ctttaggggt ggatgtcatg agtattaaac
     1261 tcaggaaggt ggcgaatact atttgcataa tttagatttg atataaaaca tggtgggata
     1321 cgaattgttc aaattgattt ttggagcagg gttataattg ggctttagtg gttggaatgt
     1381 aatttggatg aacagcccac tgagagcatg gagtttttat agtatgcgcc ggaaagagtc
     1441 ctggaagtgt gctacagaat cagagatttt gttagcaggc gactacggta ttatagtttc
     1501 accagatttt gaaccacttt acgattaccg ttggatattt ctatgaggta atttccagaa
     1561 tgcagtgatt gtaatggaat tgcaaaacta ttaatacctg ctaaagttga ttcctgttgt
     1621 gagtaaacct gctctccttt taaattgtaa atcttgattg tcacttgttc actaatttcc
     1681 atagcgaatt caacaacaac atattcttca aaaggatttg gatataaggt taacagatta
     1741 attggttcag gtttgggatt gggatagata aaacggatat acttttgttc tgacaatggt
     1801 aatccgctga tatccgcata cccacccttc atatataggc aaataaacat attttctgta
     1861 gctgtatcca atttaatatg actgccattt tgaaaaatta atgaatcacc ttggtcgagg
     1921 gtgatatata cattattaaa cttgtcgctt gcatcagata ttttaactgt atttccgata
     1981 attaattttg cattttttct taattcaatc gtactcccgg aatgcaacca taacatacct
     2041 gaacccgata aaccatattc cagttttgca ttatcatcaa caataaaagt agagcccggg
     2101 taaaaattaa aacaagatcc cggtgtaggc aaatcaatca taccacctct gtaaataaat
     2161 gccacacgat taccataaat aatatcgtag gcttcgcaaa acataccatc attcaaaata
     2221 acatttactg aatggggtat agtatcttct aagggcattc cgcctgcaat taatgtgtaa
     2281 ttttgttgta taaacgtgct ccacccggta aattcaatat caataactgt actttctggt
     2341 tgattaggtt ccggcattgc cagtaaagtg tcaattgttt ctgcagatgg atagttcggt
     2401 ttattgtaaa cacctagata atatcggggg cctccaaagt atccttctat atagatttta
     2461 taatgatcat ccgcaccaaa acttcctcca ccaataaaat cttcaattat attcgtttca
     2521 taaaccggtt gcacatatgg tggatagaaa tagattatat catcaacatc cattgccaaa
     2581 ttaaatttat aaataaagga actgatatat tgcacttcaa attttgaact tgcaaaacaa
     2641 atttcggaca tataattgct actgtttagg tcaatgtatt gttgatctat cacgtaggtt
     2701 gtgtcagtac tatccggatg ttcgagacca agaattattg cacttttgta atactccact
     2761 gtatcggctc catcttcaaa atcactaaaa tttataccat tcagggataa atgatacaca
     2821 aaattggagt ccaactttat tttattggta tcattaaaaa cagcttttaa aaataccggt
     2881 ttggaagtgt ccatttcatc aatatcaaac gatatcccga agtaatcagg atctgccttg
     2941 gttgaaatac acaatgtagt gtctaccggg ccaaaagacg tattatccgt tgtttgatag
     3001 gctatccaac ctgttgccag gtccacaaaa tgtacagggt cgtaagggca tggtacatta
     3061 atcaaatcac ctacgtcttc ctgatcggga atttgttgag cagctaaaat agttgctgta
     3121 aaaagcatca aaattgtgag tagatgtttg gagttcatgg ttgtgtattt ttaatcaaac
     3181 ttaagcaaat taaacaagat agaatatgac ttttgttatc tgattgttta gttacagcat
     3241 aatgacgggc acagagctat gatggcagga tgttttttaa tagtatgcgc cagagagagt
     3301 cctgaaacga tgctgcagaa tcagaaattt tgcagagcaa ccatcaacaa tatctcaaaa
     3361 tttaattcca tctcaaattc ggctgtttca ttggtacatt ggtacaccgg cacattggta
     3421 catcaaaggg ctgtttccgc ctcagcggac gcttcgcgaa ttagcatatc agcacattat
     3481 cacatcagca cattaatccg ccccggcgga tgcgttgcat tggtacatta gcacatcggt
     3541 acattggtac atcaaagggc tgtttccgcc tcagcggacg cttcgcgaat cagcacatca
     3601 acgaattagc acatcagcac attaatccac cgcggcggat gcgttgcatt ggtacattag
     3661 cacatcggta cattggtaca tcaaaggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnngct gttcaattag cacatcaacg aattagcaca tcagcacatt aatccgcccc
     3841 ggcggacgct acgcgcatta aaaaagcttc gtctgctccc ctttatcctt ccccttttta
     3901 tcctctaaat cccactccat ttgagtgcca acttttacat tcgatttttt ggtttcgggt
     3961 tgagaggtag tttcatcgtc cgttgtttcc cctttttttc cggttacttt tttgatttcg
     4021 tactgactta accggttgcc ctgacttttc caacctttaa tgtcgatgaa gtccgctacc
     4081 tcaattaatt cggtaacctc ctctcctttt cttcctttta ccaatactaa t
//
LOCUS       DAORKW010000325         4129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1066_length_4129_cov_3.855670, whole genome shotgun sequence.
ACCESSION   DAORKW010000325 DAORKW010000000
VERSION     DAORKW010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1066_length_4129_cov_3.855670"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3684)
                     /locus_tag="PLB46_11810"
     CDS             complement(<1..3684)
                     /locus_tag="PLB46_11810"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF77252.1"
                     /translation="MNSRILIIILAITCLLLNNLRIYAQDPLVLWDNTIGGESNDYVM
                     ASAETPDGGFILGGYSSSEPSFDKTEFVGFYDYWVVKLNSAGTIEWETTIGTTATDNL
                     TAIVPTSDGGYLLGGYTPGGITGDKTDPAIGLRDYWIIKLGPTGEIVWQKTIGGTGDD
                     YLTNILPLTGTGFIISGYSSSGISGNKTAACIGGFDYWVLKINAVGNIVWQFTIGGTG
                     NDYLFETIRNNEGNYVLAGYSNSGISGNKTEANIGGYDFWALEIDGVGTVVWQNTIGG
                     IENDYLNGICELNTGGYILAGISKSNNTVDKSENSLNYSDNCAECEFDYEYNGDDFWV
                     VKIDAVGNLIWDNTYLTGGDYSSADVAGAFQYEDNKIMIYGNAKGGYDNLETDGGNDY
                     WLLVIDTNSYVVLQQVLGGEVIWEYDDYEYEWYYTHGESYLQSCFLTSDGGFFVAGSS
                     NGNLGNDKSEAPMGEYENFDYWVLKLGPDTCIPTPIYADNDQDGEGENFVTNTCEPTY
                     GPWINNTLDCDDRYASVNSMATEICDNLDNDCDGLIDEGLVGCVGGPQITWDKTLGDT
                     LYNSLNNISATNDGGTIAVGFINAYSEENIDGIGDDDNYDIEIYKLDAEGYLQWKKII
                     TADDEDRGIRAVQTTDGGYIIGAYSGSGVSGDKTVYSYGNEDYWIIKLNTTGEIVWQK
                     AFGGTSSDILTDIESTPDGGVVIAGYSYSGISGNKTENVYGSTDFWVLKLNASGDIEW
                     QNNIGGYEYDYKPHIAFDNLGNYYVGGTSYSNTGGDKTEMHQGSGDYWAIKLNNSGSI
                     IWQNTLGGSLWDEVYDLDVDDSGSMILMGTSSSGISPDKAEVSLGKDYWVIKLKTDGS
                     IDWENTINASNDDYGTAISVSPDGGYLIGGNSNSISGTDKIEPYITPMFNSYFVSNSE
                     KNDFWILKLDSSGNIIWQNTIGGNMGDFLSSIAITNEEKIFLGGTSKSGKSADKSEMM
                     LAPVEEKIEEDDWGNKWTDYYANTDFWIVQLQPENCTLIDELCNTLDDNCNGLIDDDV
                     LETITISAAGDIEFCQGGSVSLTATYSGTSLQWQKNGTNIPGATLATYTVTTKGNYTC
                     VTTSDCGTATSDAIFVNVFKNPAANVSAAGPTTFCVGESVTLNVTPVAGCSYQWYKDA
                     SPIPGATETNYLATTAGIYKCRVTKTATGCFKTSAGIIVTVPCKEGLPDDAAGKLVQF
                     DNSFKIQPNPTNGTYTISAPSLSS"
     gene            complement(3894..>4129)
                     /locus_tag="PLB46_11815"
     CDS             complement(3894..>4129)
                     /locus_tag="PLB46_11815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77253.1"
                     /translation="IQPNPNNGTFTISAHSLSSLETTKLIIYNTTGQQIFTRELDPQQ
                     DVFEINLPEIATGMYIVKLVSGTNIYEQKLIIE"
BASE COUNT         1251 a          879 c          661 g         1338 t
ORIGIN      
        1 cgaagaaagt gagggagcgg agatggtata tgtgccgttt gtggggttgg gttggatttt
       61 aaatgaattg tcaaactgca caagcttccc tgccgcatcg tcaggtaaac cttctttaca
      121 cggtaccgta acaataatcc ctgctgaagt tttaaaacaa cccgtagccg ttttagtaac
      181 gcggcattta taaatacctg cagttgttgc caaataattt gtttcagttg cacccggaat
      241 tggcgatgca tctttatacc attgataact acatcctgca acaggagtta catttaatgt
      301 tacactttca ccaacacaaa aagtggttgg acctgcagct gaaacgttag ctgccgggtt
      361 tttgaataca ttaacaaata tggcatctga tgttgcagtt ccgcaatcac tggttgttac
      421 gcaagtgtag ttgccttttg ttgtaactgt ataagttgcc aatgttgccc cgggaatatt
      481 ggttccattt ttttgccatt gtaaggatgt accggagtag gtcgcagtga gtgacacgct
      541 accaccctgg caaaattcaa tatcaccagc agcactaatt gttattgttt ctaacacatc
      601 atcgtcaatt aaaccattgc agttatcgtc gagggtgttg caaagttcgt cgatgagggt
      661 gcaattttca ggttggagtt ggactatcca gaaatcggta ttggcataat aatcagtcca
      721 tttattaccc caatcatctt cctcaatttt ttcctcaact ggggctaaca tcatttccga
      781 tttatcggcg gacttacctg atttggaagt gccacctaaa aatatttttt cttcatttgt
      841 aattgcaatt gagcttaaga aatcacccat atttccgcca attgtatttt gccatattat
      901 atttccactg ctatccaatt ttaaaatcca aaaatcattt ttttcactgt ttgaaacaaa
      961 ataactatta aacataggag ttatataggg ttcaatttta tcggtaccgg atattgaatt
     1021 agaatttcct ccaataagat acccgccatc aggggaaaca gagatagcag taccataatc
     1081 gtcgtttgaa gcatttattg tattttccca atcgatacta ccgtctgttt ttaattttat
     1141 tacccaataa tctttaccta aacttacttc agctttatct ggagatattc cggatgaaga
     1201 tgtgcccatt aaaatcattg aacctgaatc atctacatct aaatcgtaaa cttcatccca
     1261 caatgaccca cctaaagtgt tttgccagat aatacttcct gagttattta atttaattgc
     1321 ccagtaatca cctgatcctt ggtgcatttc ggttttatct ccacctgtat ttgaatagga
     1381 agtcccacca acataatagt tgcccaaatt atcgaaagct atatgtggtt tgtagtcgta
     1441 ttcataacca ccaatattgt tttgccactc gatatcccca gaagcgttta atttaagtac
     1501 ccaaaaatct gtggagccgt aaacattttc tgttttgtta ccagatatgc ccgagtaaga
     1561 atatcctgca ataacaactc caccatccgg tgtactttca atatcagtga gtatatctga
     1621 actagtgcca ccaaatgctt tttgccagac aatttcgccc gtggtgttta atttaataat
     1681 ccaataatct tcatttccat aactatatac ggttttatca cctgatacac cggaaccgga
     1741 atatgctcca attatgtatc caccatcagt agtttgtaca gccctgatac ccctgtcttc
     1801 atcatctgca gtaataattt tcttccattg taaataacct tcggcatcca atttataaat
     1861 ttcaatatca taattatcat catctccaat accatcaata ttttcctcgg agtatgcatt
     1921 aataaagcca acagctattg taccgccatc attagtcgct gaaatattat ttaaactatt
     1981 atacaatgta tcacctaatg ttttatccca ggtaatttgt ggtccaccaa cacaacctac
     2041 taaaccttca tcaattaaac catcacaatc attatctaaa ttatcacata tttcagtcgc
     2101 cattgaattt acacttgcat acctatcatc acaatcgagt gtattattta tccagggacc
     2161 ataggttggt tcgcaggtgt tagtaacaaa attttcaccc tcgccatcct gatcattatc
     2221 agcataaatt ggggtgggaa tgcatgtatc cggaccaagt tttaataccc aataatcgaa
     2281 attttcatac tcacccatcg gggcttcaga tttatcatta cctaaatttc cattagatga
     2341 accggctaca aaaaaaccac cgtctgatgt caaaaaacaa gattgcagat aactttcccc
     2401 atgtgtgtaa taccattcat attcatagtc atcatactcc caaattactt ctccgcctag
     2461 tacctgctgc aatacaacat aactattggt atcaataact aacaaccaat aatcatttcc
     2521 tccatccgtt tccaggttat catatccgcc ttttgcatta ccatatatca tgattttatt
     2581 atcctcatat tgaaatgctc ctgcaacatc agcggatgaa tagtctccac ctgttaaata
     2641 tgtattatcc caaattaaat tgcccacagc atcaattttt accacccaga aatcatcccc
     2701 attatattca taatcaaatt cgcattcagc acaattgtca gaataattta aactattttc
     2761 ggatttatca acagtattat ttgattttga aattcctgca aggatataac ctcctgtatt
     2821 taattcacaa ataccattca ggtaatcatt ttcaatgcct ccaattgtgt tttgccaaac
     2881 tacagttccc acaccatcta tttcgagtgc ccaaaaatca taacccccga tattcgcttc
     2941 tgttttattt cctgaaattc cggaattaga ataccctgct aacacataat ttccctcatt
     3001 attacgaatg gtttcaaata gataatcatt accagttccg ccaattgtaa attgccaaac
     3061 aatatttcct actgcgttaa ttttcaagac ccaataatca aaacctccaa tgcatgctgc
     3121 tgttttattc ccggaaatac ctgatgatga atagccgcta attataaacc ctgtgcctgt
     3181 aaggggtagg atattagtga gataatcatc tcctgttcct ccaatagttt tttgccaaac
     3241 aatttcaccg gtagggccaa gtttaataat ccaataatct ctgagtccga ttgctgggtc
     3301 agttttatca cctgttatac cgccaggtgt atatcctccc aataaatacc ccccatcaga
     3361 agttggcaca attgccgtga ggttatcagt tgcagtagta ccaattgtag tttcccattc
     3421 aatagtacca gcactattta gtttaacgac ccaataatcg taaaatccaa caaattctgt
     3481 tttatcaaaa gaaggttcag aagaagaata cccgccgaga ataaagccgc catcaggggt
     3541 ttcagccgaa gccatcacgt aatcattaga ttctcctcca atagtattat cccataacac
     3601 cagcgggtcc tgtgcataaa tgcgcagatt atttaataat aaacaagtaa tagcaaggat
     3661 tataattaat attcgggagt tcatcaagta tgtttaccct aaaattacag cactttggct
     3721 agtttaaatg ccacaactta gtgaaatcga ggttttttga tgtgaaatgc ggtattcaat
     3781 tagggaatga aagtggtatt cttgagacag attaagaaaa aagctgggat tcagcatatg
     3841 aatgaacgtt cattcatata ttgaatccct aagcaaaatt tttaaaatat tatttattcg
     3901 ataattaatt tctgctcata aatatttgtc cctgaaacca atttaacgat atacatgccg
     3961 gtggcaattt cgggcaaatt gatttcgaaa acatcttgtt gcgggtctag ttcccgggta
     4021 aatatttgtt ggcctgttgt gttgtaaatt atgagttttg tagtttccaa cgatgaaagt
     4081 gagtgagcgg agatggtgaa tgtgccgttg ttggggttgg gttggatgt
//
LOCUS       DAORKW010000326         4112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1068_length_4112_cov_4.926793, whole genome shotgun sequence.
ACCESSION   DAORKW010000326 DAORKW010000000
VERSION     DAORKW010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1068_length_4112_cov_4.926793"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="PLB46_11820"
     CDS             complement(<1..141)
                     /locus_tag="PLB46_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRF77254.1"
                     /translation="MSNNKKYKIGICLSGGGARGIAHIGVLQAFESAGVFPEIVSGCS
                     AGA"
     gene            310..1644
                     /gene="creD"
                     /locus_tag="PLB46_11825"
     CDS             310..1644
                     /gene="creD"
                     /locus_tag="PLB46_11825"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HRF77255.1"
                     /translation="MNELPYSPEEQPAKSWLSTSITVKMIIIAALALLMLIPANLVQP
                     IISERENTGRSVETEVEKAWAGNQQIAGPVLTVPYVKISPKGEKQYHNKHFLPQQLAV
                     TGKLSPEKLERSIYEITVYDAALNVNGNFDVAAIQSAQFDGTPLWENAYLSIAINDLS
                     GVQNALNFKLDNEQLPIKSGLKSKILGESGVTLPIDLTTHFFTNWQFNFDLQVKGTSE
                     IAFLPIGNNTSVNLSSPWSAPGFNGAFSPDNKTIQNDGFTAQWDILELNRNYPQMWDD
                     DAYTSQLQESSFGVDLVNTTNDYQKAMRSVKYAILTIGLTFLVFFLVEIINGNRIHPF
                     QYILVGLALSLFYVLLISISEHIDFNIAYLIAASVIVTMITLYSLAVFTQKKLSVLLF
                     FILSGLYGFLFVILQANDYALLLGSIGLVIMLALTMYLTRKINWYQVKAHVA"
     gene            complement(1720..2838)
                     /locus_tag="PLB46_11830"
     CDS             complement(1720..2838)
                     /locus_tag="PLB46_11830"
                     /inference="COORDINATES: protein motif:HMM:NF027711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5005 domain-containing protein"
                     /protein_id="HRF77256.1"
                     /translation="MFKKVNLIPVLSLTHLLFFSACQKEVPVTVDNEADEVHYALAGV
                     STKSANKFTNLFNRFGNGWTGGDATYSIALPDGRTVWMFGDSFLDTVYADYSRPSSGL
                     IRNCFMVQTGGSMTTLCGNTIDAPQALVDTDDPSDEWYWPGDATVIGENLYVFFMYFI
                     RTGPDAFDFAYLRTDLVTFSLPTITETSRNTVYTNNDILWGASLMEDGDYIYVYGAEQ
                     AFLTKYAHVSRYPADDITAAPESWNGTSWVSTEPATNVGRLEKQSGLPVEASAQFAVF
                     YSGGKYRLVTQEDLFSPNIYTWEAVAPTGPWKKRQTVYVTPETGGDLWTYNAFVHPQF
                     TTADGYMLLSYNENSMNFFDLFEDARIYRPKFIYFKYL"
     gene            2927..3952
                     /locus_tag="PLB46_11835"
                     /pseudo
     CDS             2927..3952
                     /locus_tag="PLB46_11835"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
     gene            4030..>4112
                     /locus_tag="PLB46_11840"
     CDS             4030..>4112
                     /locus_tag="PLB46_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRF77257.1"
                     /translation="MKGIILAGGSGTRLHPITLAISKQLMP"
BASE COUNT         1283 a          732 c          791 g         1306 t
ORIGIN      
        1 ggcgcctgca ctgcaaccgg atacaatttc ggggaataca ccggcgcttt caaatgcctg
       61 caaaactcct atatgtgcaa ttccacgcgc acctccgcct gaaaggcaaa tgccaatttt
      121 atactttttg ttattgctca tactgtctgt cacctcattt ccagccgaaa cccggcttta
      181 gcaaatgtac catattcaac ggcaatttgg tttggtaaaa ggtttttcgg atggcaaaaa
      241 aataaaaagt gttaaaaact tgtttattct tggaaaaaca aagagctttg atgcacaaag
      301 taaataatta tgaacgaatt accttattct cccgaagagc aacctgcaaa gtcatggtta
      361 agcacttcca ttaccgtaaa aatgatcatc attgctgcat tagccctgtt aatgctgatt
      421 ccggcaaacc tggttcaacc tatcatatct gaaagggaaa acaccgggcg aagcgttgaa
      481 accgaggtgg aaaaagcctg ggcaggaaat cagcaaattg caggaccggt attaacggtt
      541 ccgtatgtaa agatttcacc aaaaggtgaa aaacaatatc acaataaaca ttttcttcct
      601 cagcaacttg ccgtgactgg aaaattaagt cctgaaaaac ttgaacgcag tatttatgaa
      661 attactgttt atgatgccgc attaaatgta aacggcaatt ttgatgtggc agctattcag
      721 tcggcacaat ttgatggcac tccgctgtgg gaaaatgctt atttatctat tgctatcaac
      781 gacctgagtg gcgttcaaaa tgcattgaat tttaaattag ataacgaaca attaccaata
      841 aaatcaggtt taaaaagtaa aattttaggt gaaagtggtg ttacattacc aatagattta
      901 accacacatt tttttactaa ttggcaattt aatttcgatt tgcaggtaaa gggcaccagt
      961 gaaattgcat ttttaccaat tggcaataac acatctgtaa atttatcttc accatggagt
     1021 gcaccgggat ttaatggtgc attttctccg gataataaaa ccatacaaaa cgatggattt
     1081 actgcgcaat gggacatatt ggaattaaac agaaattacc ctcaaatgtg ggacgatgat
     1141 gcgtatacat cgcaattgca ggaatcatca tttggtgttg atttggttaa taccaccaat
     1201 gattaccaga aagcaatgcg ctcggtaaaa tatgccattt taactattgg cctgacgttt
     1261 ttagtatttt ttctggtaga aataattaat ggtaaccgta tacatccatt tcaatatatt
     1321 ttagtgggat tggcattgag tttattttat gtattactta tttctatatc agaacatatt
     1381 gattttaata tcgcttattt aattgcagcg tcagtaattg tgacaatgat tacactttac
     1441 tcactcgcgg tttttacgca aaaaaaatta tcggtattgt tgttttttat tttatcaggg
     1501 ttgtacggat ttttatttgt catcctgcaa gccaacgatt acgcattatt acttggaagt
     1561 attggattag taattatgtt agccttaaca atgtatttaa ccagaaaaat aaactggtat
     1621 caggttaaag cacatgtagc ataattcgaa aaaaattaat ggataaaaaa aggttaccac
     1681 attggtaacc tttttttgtt aatataactt gttaagattt tacagatatt taaaataaat
     1741 aaattttggc ctgtatattc tcgcatcttc aaataaatcg aaaaagttca tgctgttttc
     1801 attgtaagat aacaacatat atccatcggc agtagtaaat tgcggatgaa caaatgcatt
     1861 ataagtccat aaatcgccac ctgtttcagg tgttacataa acggtttgtc gtttcttcca
     1921 tgggcctgtt ggagctactg cttcccaagt gtaaatattc ggactaaata aatcttcctg
     1981 agtaacgagt cgatattttc ctcccgaata aaaaacagca aattgtgccg aagcttctac
     2041 aggtaaacca ctttgttttt ccagtctgcc aacattagtt gcaggttctg tgcttaccca
     2101 tgaagtgcca ttccaagatt caggagctgc tgtaatgtcg tcggccggat atctgcttac
     2161 atgcgcatat ttggttaaaa aggcttgctc agcaccataa acataaatgt aatcgccatc
     2221 ttccattaaa gaagcgcccc ataaaatatc attattggta tacacggtat ttcttgaagt
     2281 ttcagttatg gtgggtaagc taaaagttac taaatcggtg cgcagatagg caaaatcgaa
     2341 ggcatccggt cctgtgcgga tgaagtacat aaaaaacaca tataaatttt caccaataac
     2401 tgttgcatcg cccggccaat accattcatc tgaagggtca tcagtatcaa ccagtgcttg
     2461 cggcgcatca atggtgttgc cgcatagggt tgtcattgag ccacctgttt gcaccataaa
     2521 acaattgcga attaatccgc tgcttgggcg cgaataatcg gcataaaccg tatctaaaaa
     2581 cgaatcgcca aacatccaga cagttctgcc atcgggcaaa gcaattgaat aagtagcatc
     2641 accacctgtc cagccattgc caaaccggtt aaacaaattg gtaaatttat tggcggattt
     2701 agtgcttact ccggctaacg cataatgcac ttcatctgct tcattgtcaa cggtaacagg
     2761 tacctccttt tgacatgcag aaaaaaatag caggtgtgtt aatgagagga caggaattaa
     2821 gtttactttt ttgaacatag tggtgtttta ttcagtttag atgaaaaaaa taaaattacc
     2881 ggacattaaa attttgcctt gtaattttgc ataaatagct aaagttatga aatctgtaag
     2941 aatttggttg tggattggcg tttttatggt gttggtgcaa gtaatgttgg gtggaattac
     3001 ccgcttaaca gaatcaggtt tatctattac cgaatgggat gttgtaatgg gcagttttcc
     3061 gccaacttcc tctgagggat ggcaacaaga atttgaaaac tatcaaaaaa gtgcacaatt
     3121 cgaaaaagta aataacgatt ttactgttga acaattcaaa agtatttatt ggtgggaatt
     3181 tattcatcgt ttatgggcca gattaattgg ctttgtattt ttatttccgt ttatttattt
     3241 tttaatcaag aaaaaattta atggcagctg gataaaaaaa ttattatttg tttttttatt
     3301 aggcggatta caaggatttg taggatgggt gatggtggcc agcggtcttg atggcgaccc
     3361 atgggtagat ccaagtaagt taactatcca tttattattg gcattgctgg tatatatgta
     3421 tttaatatgg ctggcattat cgtaccgaca taaaccggta attaccgatc atcctaatgc
     3481 acatcgttcg ataaaatggg tattggtttt ggtatttatt caaatcggat taggtggatt
     3541 aatggcaggt accgtataag ggcgtttgta taataactct cctttaatga atggaagggt
     3601 tattcctgag ggtgcgtttc aacaaatttt tagtgcagaa aaactcgttg cattatcgcc
     3661 ggaaattaat ttcattcatc gcacatttgc tttggttgtc actttgatga ttatttattt
     3721 ttggctgcaa aatcgaaaat tcagctctcc aataattcat aaactgaaca actttttatt
     3781 aataggggtg cttattcaat ttttacttgg tgtactcact ttattaggtg ctaaaacaag
     3841 tattccagtg tttttgggtg tagcacacca gattaccgct tgtttattgc ttggagtggt
     3901 agttgccatt atgttttatg ccaaaccaag aagcggttac acgcaaccgt aagggactaa
     3961 acctcatatc ctgctccagg atgcggctat aaaaatttga accgctatct ttgcccaaaa
     4021 caatcaactg tgaaaggaat tattcttgca ggaggatcag gaacacgtct gcatcccatt
     4081 actttggcca ttagcaaaca attaatgcca at
//
LOCUS       DAORKW010000327         4111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1070_length_4111_cov_3.158284, whole genome shotgun sequence.
ACCESSION   DAORKW010000327 DAORKW010000000
VERSION     DAORKW010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1070_length_4111_cov_3.158284"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..448)
                     /locus_tag="PLB46_11845"
     CDS             complement(83..448)
                     /locus_tag="PLB46_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77258.1"
                     /translation="MENSTSQRTTPSYIWFVLHFVITLLAWIGPFIFDWYLMITAYCI
                     VLLQFLVFNRCLMNIKHDLSEDEHMTFYAYLFESVGIHFNRRKLKIFVRKWLYACLGV
                     FTLILQLLLHYQPLLSLSL"
     gene            646..1125
                     /locus_tag="PLB46_11850"
     CDS             646..1125
                     /locus_tag="PLB46_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRF77259.1"
                     /translation="MSQQLSTKVAAVFHQRFLNLYDGKIPSAEAIVNTPFETLRSIGL
                     SNAKAQYIQNVAQFEIQFGMQAAVLKKMGDEEVIQYLTQIKGVGRWTVEMLLMFTLGR
                     ENVFPVDDLGIQQAMINLYQIKYKDKKSLLNKMQKIAASWAPYRTYACMHLWHYKDN"
     gene            complement(1334..1639)
                     /locus_tag="PLB46_11855"
     CDS             complement(1334..1639)
                     /locus_tag="PLB46_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77260.1"
                     /translation="MKKKILIGSFVVLTGAMVGLFAMSFSNNNNSNVNTVPVEQVSTT
                     ATTPEHCAPSECSAEKASTCPYSKTNTTQEASANCPATADCPPSKCSSTEKSDKATL"
     gene            1786..3855
                     /locus_tag="PLB46_11860"
     CDS             1786..3855
                     /locus_tag="PLB46_11860"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77261.1"
                     /translation="MKKTILTIAIGFSAFSLFAQQATNVETGSNTNLTAQDAALLPKE
                     KSDDFEWVVEQFADLGILRYQIPGWDKLTVNQKLFVYYLTEAGYAGRDMIWDQNYRHN
                     LEIRHALDQIVRDYKGNRENGDWDKFITYTKRVWFSNGIHHHYSTDKIMPEFSKTYFE
                     SLLSATNTKLSADVIKIMFDPQIDNKKVNLDANADLLLSSATNFYGVDVSQKDAEAYY
                     ATKIDPNDTKAISHGLNSQLVKTPNGIEERVYKVNGMYHESIIEVVGWLKKAMTVCEN
                     DQQAYALKLLIQYYETGDLKIWDDYNIAWVQTTNGDIDYINSFIEVYNDPLGYRGSFE
                     SIVEIKDFEASERMSKLADHAQWFEDNSPLMPEHKKPTVVGVSYKVVNVAGEAGDASP
                     ATPIGVNLPNANWIRADYGSKSVSLGNIVYAYEQAGSSGILDEFCYTAEEKERAKKYG
                     SLAGKMHTAMHEVIGHASGVINPGVGTPKETLKNYANTLEEARADLVGLYYIMDPKLV
                     EWGLMPSLEVGKAQYDGYIRNGLMTQLRRIEPGKTVEESHMRNRQLIAAWAFEKGKQG
                     NVIERKYENGKTYFVVNDYEALRKIFGELLREVQRIKSEGDFKAGQALVENYGVQVDE
                     QIHKEVLTRTSTMNIPPYFGFVNPILTPVKDIEGNVIDVKVTYADSFTQQMLYYGEKY
                     SFLKPGK"
BASE COUNT         1431 a          614 c          809 g         1257 t
ORIGIN      
        1 ataacgtttt atcatgaaac taaatttttg cgaagataga acacctgtcc gaatattagg
       61 tgttggacga tgttacataa tgttaaagtg aaaggctgag taatggttga taatgcaaca
      121 gtaattgtaa gatgagggta aatacaccaa ggcaggcata taaccatttc cttacgaata
      181 ttttgagctt tcgtcggttg aaatgaatac caactgattc gaataaatag gcgtaaaacg
      241 tcatgtgttc atcttcagac aaatcgtgtt ttatattcat caaacagcga ttaaaaacca
      301 aaaattgtaa taacacgatg cagtaagctg taatcattaa ataccaatcg aaaataaacg
      361 gaccaatcca tgctaaaagt gtaattacaa aatgcaggac aaaccaaata taggaaggtg
      421 ttgttctttg ggaagtgcta ttttccattt ggtgaagata ttaacttgcc tgaattttac
      481 aaaaacgaac aaaaaaaact ttaatttcaa cccaattaaa aagattacaa tggattatgt
      541 gcaacattta aacaggatga acgattagca agtttaatca ggcaacatgg cagtttttca
      601 ataaagcaac gcaaacaggt gtatttccat ttgtgcagtt caataatgag tcagcaatta
      661 tcaactaaag ttgcagctgt tttccatcag cgatttttga atttatatga tgggaaaatt
      721 ccaagtgctg aagccattgt aaacactcca tttgaaacct taagaagcat aggattgtcg
      781 aatgcaaagg cgcagtatat tcaaaatgta gcgcagtttg aaatacaatt tggtatgcag
      841 gcggcagttt taaaaaagat gggcgatgaa gaagttattc aatatttgac acaaataaaa
      901 ggtgtgggca gatggacagt agaaatgttg ttgatgttta ccttaggtag agaaaatgtt
      961 tttcctgtag atgatttagg catacaacaa gcaatgatta atttatatca gataaaatat
     1021 aaagataaaa agtcgttgct gaataaaatg caaaaaattg cagcatcatg ggcaccttat
     1081 aggacatatg cttgcatgca tttgtggcat tataaagata attaaaactg ccggtgcaaa
     1141 tccggcaact tattcaaaac ccatgaaaat cacgattatt tttattccgg attttgttgc
     1201 taaactaata aggtaataat tgttttaaat tggtatcagt ttatctgatg aaagataaat
     1261 atgctaaatc aaatgagcat caaaaaaaag ccgcaataaa aattattgca gctataaaat
     1321 agtttgataa aatttataaa gttgctttgt ctgatttttc agtgcttgaa cattttgaag
     1381 gcggacaatc tgctgttgct gggcaatttg cagaagcttc ctgtgtagta tttgttttgc
     1441 tgtaaggaca tgttgatgct ttttcagcgc tacattcaga cggagcacag tgttcaggtg
     1501 ttgttgctgt tgttgaaacc tgttcaacag gaaccgtgtt tacatttgaa ttattgttat
     1561 tactgaacga catggcaaat aaacctacca tagcaccggt aagtactaca aaagatccaa
     1621 tcagaatttt ctttttcatg tttatttatt ttgattaaag atacagtttt tatcaataac
     1681 gtaaggtgta tggaataaat tttattaacc gtttagtatg tttaaacgga agtaattagt
     1741 aaattcagca cgatttatgg agatttgtaa cataaactat caaaaatgaa aaagacgatt
     1801 ttgactattg ctatcgggtt ttcagccttt tcgctgtttg cgcagcaggc tactaacgtt
     1861 gaaaccggca gcaacactaa tttaacagct caggatgcag ctttattacc caaagaaaag
     1921 tccgatgatt ttgaatgggt tgtggagcaa tttgctgact taggtatttt aagatatcag
     1981 ataccgggat gggacaagct gacggttaac caaaaactgt ttgtttacta tctcactgaa
     2041 gcaggatatg ccggcagaga catgatttgg gaccaaaact atcgccataa tctggaaatc
     2101 agacatgcat tagaccaaat tgtgagagat tacaaaggca atcgcgaaaa tggcgattgg
     2161 gataaattta tcacctatac gaaacgtgtt tggtttagta atggaattca tcatcattat
     2221 tcaacggata aaataatgcc tgaattttca aaaacatatt ttgaaagttt actttcggct
     2281 actaatacta aattatcggc tgatgtaatt aaaatcatgt ttgatccgca aatagacaac
     2341 aaaaaagtga acctcgatgc gaatgctgat ttattactta gctctgctac caatttttat
     2401 ggtgttgatg tgtcgcaaaa agatgcagag gcgtattatg caactaaaat tgatccgaat
     2461 gacaccaaag ctatttccca tggtttgaat agtcagttgg ttaaaacacc aaatggtata
     2521 gaagagcgcg tttacaaagt aaatggcatg tatcacgaat caattataga ggtagttggt
     2581 tggttaaaaa aagctatgac agtttgtgaa aatgaccaac aagcttatgc gttaaaatta
     2641 ttaatccaat attatgaaac aggcgattta aaaatttggg acgattataa tattgcttgg
     2701 gtgcaaacca ccaatggaga cattgattac attaattcat ttattgaggt ttataacgac
     2761 ccattaggat acagaggttc gttcgaatcg atagttgaaa taaaagattt tgaagcttct
     2821 gaacgcatga gtaaattagc agaccatgca caatggtttg aagataattc accgttaatg
     2881 cctgagcata aaaaaccaac agttgttggt gtttcttata aagtagtaaa tgtggccggt
     2941 gaagccggtg atgcatctcc tgcaacacct attggtgtta acttaccaaa tgcgaactgg
     3001 ataagagctg attatggttc taagagtgtg agtttgggca atatcgtata tgcctacgag
     3061 caggcaggta gttcaggtat tttagacgaa ttttgttaca ctgccgaaga aaaagaaaga
     3121 gctaaaaaat acggttcttt agccggaaaa atgcatactg ctatgcacga agttattggc
     3181 catgcaagtg gtgttattaa tcctggagta ggcacaccaa aagaaacttt aaaaaattac
     3241 gccaacacat tagaagaagc aagagctgac cttgttggtt tatattatat catggaccca
     3301 aaattagttg aatggggttt aatgccatca cttgaagtgg gtaaagcaca atatgatggt
     3361 tatatccgca acggtctaat gacacaattg cgcagaattg agccgggtaa aacagttgaa
     3421 gaatcacata tgcgtaatcg tcagttaatt gctgcatggg cttttgaaaa aggtaaacaa
     3481 ggtaatgtaa ttgaacgcaa atatgaaaat ggtaaaacct attttgtagt aaacgattat
     3541 gaagctttgc gcaaaatttt tggagaatta ttacgtgagg ttcaacgtat taaaagcgaa
     3601 ggtgatttta aagcaggaca ggctttagtt gaaaattacg gtgtgcaagt tgatgaacaa
     3661 atacacaaag aagtattaac cagaacttca accatgaata ttccacctta cttcggtttt
     3721 gtaaatccta ttcttactcc ggtaaaagat attgaaggta atgttattga tgtaaaagta
     3781 acttatgccg atagctttac acaacaaatg ttgtattacg gtgaaaaata tagtttttta
     3841 aaaccaggaa aataattact taataatatt aaaaaagacc atcgtaactg atggtctttt
     3901 tttttgagta aaagaatacg ttggcaattt tacattatta ctgaaatttt atttgtgtgt
     3961 gccggcatct tcaacaactg atgccgacta agttttgggt tggttttaac tgtttgttta
     4021 cgcaattgac cacatttcca catttggtga tatatatcat acttcccaac gatgacctgt
     4081 gatagttttg tggtataaaa caactagtat a
//
LOCUS       DAORKW010000328         4084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1078_length_4084_cov_5.039464, whole genome shotgun sequence.
ACCESSION   DAORKW010000328 DAORKW010000000
VERSION     DAORKW010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1078_length_4084_cov_5.039464"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="PLB46_11865"
     CDS             <1..1351
                     /locus_tag="PLB46_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77262.1"
                     /translation="TTAGGGGGSYTAGTGINITGTIISNTGDTNAADDVSTSTNFGGD
                     VTGIYNNLQLASGSVGSAEIADGTVSSTDITNATIAAADLSSMGASTGQVMQWNGTSW
                     VATTPAAGAESDPQVGANTTDLVPRWDGTALVSGAIADNGDNISVGWNSGINTAVSAA
                     FYNNESAFLDEEIALKAVDHTLTIGGTSTVHAFTNLGYNTSGLIFFDTPISHAATWAA
                     ATSTDNAASVYATNTSTGASNYGLIAKATGAGTTNFGIWAKASGATNNYALIVPSDGG
                     NVGIGTSAPNVRLHVNSLLGEDAFRVQVSGSTKLFVQSNGGTSFGGLTAAPSNGVYVS
                     GNVQVGAEPVPAGYKMSVDGKFICEEVKVQLSADWADYVFEENYNLMSIDEVAKFIAT
                     NKHLPNIPSADELEKNGLDLSAMNILMMQKIEELTLYVIELDKQNKLLQQEVELLKK"
     gene            1379..3085
                     /locus_tag="PLB46_11870"
     CDS             1379..3085
                     /locus_tag="PLB46_11870"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRF77263.1"
                     /translation="MKKITMNIMLLCGLITIGNVTTTRAQSAVLLDDGNKQVECVTEE
                     NYQEFIRPQLKASINKLRQEGKLNFANDGTNAKVGDGTVPLSWPLRMHGDYKDIDGVN
                     DYFVITNFADLNHDEYYRLDWDCKTYENARNYDQHNGADILPYPFTWEMMDDESVEII
                     AAADGEVIQRYDGNTVDRNCAAPHVFNFEPFNGGYYGNFIALMHSDSSITVYAHMKNG
                     TVADLEPGDFVEAGQFLGKMGSSGNSTAPHLHFEVRPCVDCQYIEPWFDAAGCNDDVT
                     ESQWISQIPYNDPKILRVATHLNIPVYKTCAEYEAGANEDENLVNHFSTTDNLLISVA
                     IGDLFGDIPLYMEIINSAGTVVASDIYNSPYYQEYKATVLFTELLTGYASGTYKIRIS
                     YNGRLAYHYFTVGCPAATTLIGTHTGVRGYLTGDYIASTASIAGLSTNNIFYQAENYV
                     RLNPGFVAAQNSKFHAQINDCTVGGMREMEEEIIGDNNLLVSPNPSFGIFSINYTADV
                     NSNMRLIIRNLYGNVVYESELFENIDQINQTIDLTNYAKGIYLIEIQKPEGSISQQVV
                     IQ"
     gene            3160..3552
                     /locus_tag="PLB46_11875"
     CDS             3160..3552
                     /locus_tag="PLB46_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77264.1"
                     /translation="MALTETDKATIFNTLVEALQTCTPPMVVTKNTNAVFELIGNKPV
                     PYGYKKEIIPGMYFSSVVARKDMVSFYVFPLYGQEDLFKDIAPIAMKCLKGKTCFNFK
                     KTEQVNKKEIVAILKQGVKIWKQMGYLK"
BASE COUNT         1307 a          721 c          828 g         1228 t
ORIGIN      
        1 gactaccgca ggcgggggag gtggaagtta cactgccgga acaggtatta atattaccgg
       61 taccattatt tcaaacaccg gtgatactaa tgctgctgat gatgtaagta catctaccaa
      121 ttttggaggc gatgtaacag gcatttataa taatcttcaa ttagcaagcg gtagcgtagg
      181 ttctgcagaa attgcagatg gaacagtttc atctaccgat ataaccaatg ccacaattgc
      241 cgcagccgat ttaagttcaa tgggtgcaag cacaggacaa gtaatgcaat ggaacggaac
      301 ttcatgggta gcaacaactc ctgctgcagg cgcagaaagt gatccgcagg tgggcgcaaa
      361 tactacagat ttagttcctc gttgggatgg tactgcatta gtaagcggtg caattgctga
      421 caatggtgat aatatttctg ttggttggaa tagtggaatt aatacagctg tttctgctgc
      481 attttataat aatgaaagcg catttcttga tgaagaaatt gctttaaaag cagttgatca
      541 cacccttaca attggaggaa catcaactgt gcatgcattt accaatcttg gatataatac
      601 ttcaggttta atattttttg atacaccgat ttcacatgca gctacatggg cagcagcaac
      661 ttcaactgat aatgcagcat cggtgtatgc aaccaatact tctaccggtg caagtaatta
      721 tggtttaatt gctaaagcaa ccggcgcagg aactaccaac tttggcatat gggcaaaagc
      781 ttccggtgct actaataatt atgcgctaat tgttccatcc gatggtggta atgttggtat
      841 cggaacttca gcgcctaacg tgcgtttgca tgtgaatagt ttacttggtg aagatgcttt
      901 ccgtgtgcag gtttcgggct caaccaaatt gtttgtgcag tctaatggtg gtacatcatt
      961 cggcggttta actgcagcgc catcaaatgg tgtttatgtt tcaggaaatg ttcaagttgg
     1021 cgcagaacct gttcctgcag gatataaaat gagtgttgat ggtaaattta tttgcgaaga
     1081 agtaaaagta caattgagtg ccgattgggc ggattatgta tttgaagaaa attataacct
     1141 gatgagcatt gatgaagttg ctaaatttat tgctaccaat aaacatcttc ctaatattcc
     1201 atctgctgat gagttagaaa aaaatggtct cgatttatct gcaatgaata ttttaatgat
     1261 gcaaaaaatt gaagaactta ccttgtatgt tatcgaatta gataaacaaa ataaattgtt
     1321 acagcaggaa gttgaattac ttaaaaaata attgttcatt aaattaaatt caattgttat
     1381 gaaaaaaatt acaatgaaca tcatgttgtt atgtggtttg ataaccatcg gcaatgtaac
     1441 aacaaccaga gcgcaatctg cagtattgct ggacgatgga aacaaacaag tagaatgtgt
     1501 tactgaagaa aactatcagg aatttattcg cccgcaatta aaggcaagta taaataagtt
     1561 acgacaggaa ggaaaattga attttgccaa tgatggaact aacgcaaaag ttggtgatgg
     1621 cacagttccg ctgtcgtggc cgttgcgtat gcatggtgat tataaagata ttgatggggt
     1681 taatgattat tttgtgatta ccaatttcgc tgaccttaat cacgatgaat attatcgact
     1741 agactgggat tgcaaaacgt atgaaaacgc caggaattat gaccagcata atggtgccga
     1801 tatattgcca tatccattta catgggagat gatggatgat gaaagtgttg agataattgc
     1861 tgcagctgat ggcgaagtaa ttcaacggta cgatggaaat acagtggacc gaaattgcgc
     1921 cgcaccacac gtgtttaatt ttgaaccatt taatggaggt tattatggca actttattgc
     1981 actcatgcat agcgacagca gtattactgt ttatgcgcac atgaaaaatg ggaccgttgc
     2041 agaccttgaa cccggcgatt ttgttgaagc cggtcagttt cttggaaaaa tgggaagttc
     2101 cggaaattct acagctccgc atttacattt tgaagtgcgc ccttgtgtcg attgccaata
     2161 tattgaacca tggtttgatg cagcaggttg caacgatgat gttacggaga gtcagtggat
     2221 ttctcaaatt ccgtataacg atcccaaaat tttacgtgta gccactcatc taaatattcc
     2281 tgtgtataaa acatgtgctg aatatgaagc cggtgcaaac gaagatgaaa atctggtaaa
     2341 ccatttttct acgactgata atttattaat ctctgtagct attggtgatt tatttggtga
     2401 cattcctctt tatatggaaa ttatcaattc agcgggaact gttgtagcat cagatattta
     2461 taattcacca tattaccagg agtataaagc aacagtttta tttaccgaat tgctaactgg
     2521 atatgcttcc ggaacgtata aaattcgtat ttcctataat ggtagactag cttatcatta
     2581 tttcacagtt ggttgccctg cagctacaac gttgattggt acacatacag gagttagagg
     2641 ttatttaact ggcgattaca ttgccagcac agcttcaata gcaggtttaa gtactaacaa
     2701 cattttttat caggcagaaa attatgtaag attaaatcct ggatttgtag cagctcaaaa
     2761 cagtaaattt catgcacaaa taaatgattg taccgtaggt ggaatgcgtg aaatggaaga
     2821 agaaataatt ggcgataaca atttattggt ttccccaaat ccgagctttg gtattttctc
     2881 cattaattat acggctgatg ttaacagcaa tatgcgttta atcatccgca atttatatgg
     2941 taatgtggtt tatgaatcag agttgtttga aaatattgat caaataaacc aaaccatcga
     3001 tttaaccaat tatgccaaag gtatatacct cattgaaatt caaaaacctg agggctccat
     3061 ttcgcaacag gtagttattc aataaactat tttttggttc attattctga aaccggctca
     3121 attttgggcc ggttttttta tatttgtcaa aacactcgta tggcactaac tgaaactgac
     3181 aaagcaacta tatttaatac gttggtggaa gcattacaaa cttgtacacc accaatggtt
     3241 gttaccaaaa acacgaatgc cgtatttgaa cttattggca ataaaccggt gccttatgga
     3301 tataaaaaag aaataatccc gggcatgtat ttttcttcag ttgttgccag aaaagatatg
     3361 gtgagttttt atgtatttcc actttatgga caagaggatt tatttaaaga tattgctcca
     3421 atagctatga aatgtctgaa agggaaaacc tgttttaatt ttaaaaaaac cgaacaggta
     3481 aataaaaaag aaatcgttgc cattctcaaa caaggggtga aaatttggaa acaaatgggg
     3541 tatctgaaat gaggtaatta gcaaattagc tgattagcaa atggggttca ctgcgtgaat
     3601 ggttttaatg cttgcgcatt aaaatgggta gatgggtatt ttctgcaata ttatatttcc
     3661 caaaaaccat ggtcaacacg tgggttgacg ggtacaggtg ttccgcatta cataccaaca
     3721 caaaaacccc ggctgtttcc atcagtacat ccacctattg gtacatcggt acattcacac
     3781 attggtacat cattcggctg ttaatcagca catcagcaca taatcacatc aacacatcca
     3841 cccattggta catcggtaca ttcacacatc ggaacatcca ccattggtac atcggtacat
     3901 tcacacattg gtacatcatt cggctgttaa tcagcacata atcacataat cacatcaaca
     3961 cattaaaaaa cccctgaatc ctcagaggtt ttaaaaattt catgcatata caattggtga
     4021 aaaaatcccc tcactcaatc actctcttaa atcaattact tcaacacctg gatatttcga
     4081 tttg
//
LOCUS       DAORKW010000329         4047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1093_length_4047_cov_4.700651, whole genome shotgun sequence.
ACCESSION   DAORKW010000329 DAORKW010000000
VERSION     DAORKW010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1093_length_4047_cov_4.700651"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PLB46_11880"
     CDS             <1..1066
                     /locus_tag="PLB46_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRF77265.1"
                     /translation="YLQQYYGNPSSIHSVGRETRAAIEKARKLIAGLFHVSPGEIFFT
                     SGGTEANNMALQCSVRDLGVTRIITSPIEHHAVTHTVEALKNEGKIQLDYVALNSDGS
                     VNYEHLEQLLANNTAKTLVSLMHANNEIGTLLDIDKVSNLCIAHNAYFHCDTVQTVAH
                     YQLDLQKTKVHFISCAAHKFHGPKGVGFIYINGEAKIKPLIHGGAQERNVRAGTENLY
                     GIIGLAKALEIAYAELDTVSEHIKNLRDHMMKRLLEEIPGVQINSDNNGLYTVLNVNF
                     PASDKNELLLFNLDIAGIAVSGGSACSSGTDIGSHVIAQLKRAPGSTAVRFSFSKFNT
                     IEEIDFAVDKIREMMPVAVS"
     gene            1122..2405
                     /locus_tag="PLB46_11885"
     CDS             1122..2405
                     /locus_tag="PLB46_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRF77266.1"
                     /translation="MANRRSFLKKSAALFSVANLPQFVHSEEINAAIQRAQKSTPEIL
                     AADEDFWATIKSAYTASNTIINLNNGGVSPSPKVVQDAMQRYSQLANEAPSYYMWQIL
                     DKGRENLRAKLANLAGCDPNEIAINRNTTEALDNVIFGLDFEKGDEVLLTKQDYPNMI
                     NAWKQREKRDGIVLKWIDLELPSDDTDYLVKKYTEQITSKTKLVQVNHIINWTGHINP
                     VKEIAAAVHAKGPEVMIDGAHTFGHFVFSVPELGGDYYGTSLHKWMSATIGSGMLWIK
                     KEKIHKVWPLMGAPDTLDKNDIRKFETLGTRNFPIEYSIATAIDFHQMIGPERKEARL
                     RYLRDYWVNKCSDIPTFKLNTSLTKSYSCAIANFSLEGIEPGDISTKLFTDHGVHCVS
                     IVWENIKGVRIAPHVYTSLYDLDILVKGIHKIAGK"
     gene            complement(2406..3422)
                     /locus_tag="PLB46_11890"
     CDS             complement(2406..3422)
                     /locus_tag="PLB46_11890"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HRF77267.1"
                     /translation="MTKSGMESGKTIEGLIAAGAFIQTKPDDLMAVLHRTEINNPWFT
                     QLESLRMLDSMCNKLLAADTLVTWIAQYNTNPAAPKTIGLVLAGNVPFVGMHDLICVL
                     ASGHNAKVKLSSKDMFFFPWFKQVLVKINTDFEQRIQFVDKLENFDAVIATGSNNSAR
                     YFEYYFGKYPHIIRKNRTSVAILTGNETEEQLNNLGKDVFYYYGLGCRNVGKVFFPEQ
                     FDRQLLFKYWEDYRYVTDNTKFKNNYDYNRALLLLNSTNYLTNDFYMLVESTELFSPL
                     SVLYCETYKSEQDLQEKLDIILPDLQCIVAAAPGNTAFGQTQYPGLNDYADHVDTMAF
                     LTSL"
     gene            3577..3927
                     /locus_tag="PLB46_11895"
     CDS             3577..3927
                     /locus_tag="PLB46_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRF77268.1"
                     /translation="MMAIHITSDCINCGACEPECPNNAIYEGGVEWAFADGTTVKGAV
                     TMIDGSTMDADQKNAPIADDIYYIVPDKCTECQGFHEEPQCAAVCPVDCCLPDPDHVE
                     SEEQLLAKKSKMHI"
BASE COUNT         1297 a          744 c          789 g         1217 t
ORIGIN      
        1 ctatttacaa cagtattacg ggaatccatc ttctattcac tctgttggtc gcgaaaccag
       61 agcagctatt gaaaaggcaa gaaaactcat tgccgggttg ttccatgtgt caccgggtga
      121 gatttttttt accagcggag ggacggaggc aaacaatatg gctttacaat gcagcgtgcg
      181 cgatttaggg gtaacccgta tcataacatc gccaatagaa catcacgcag taacacatac
      241 ggtagaagca ctcaaaaatg aaggcaaaat tcaactcgac tatgtggcat taaacagtga
      301 cgggtcagtt aattatgaac atctggaaca attactggct aataatacag cgaaaacctt
      361 agtgagttta atgcatgcca ataacgaaat aggcacacta ctcgatattg ataaagtgag
      421 taatttatgc atcgctcaca atgcttattt tcattgtgat actgttcaaa cggttgcgca
      481 ttatcaactc gatttacaaa aaacaaaagt gcattttatt tcatgtgctg cacataaatt
      541 ccacggacca aaaggagtag gatttatcta tatcaatgga gaagctaaaa ttaaaccatt
      601 gattcatggc ggcgctcagg aaagaaatgt acgcgcagga actgaaaatt tatatggaat
      661 tataggttta gcaaaagcac ttgaaattgc ctatgcggaa ttagatactg taagtgaaca
      721 tatcaaaaat ttgcgcgatc atatgatgaa aagattactg gaagaaattc cgggtgtgca
      781 aattaattct gataataatg gattgtatac ggttttaaat gtaaacttcc cggcatcaga
      841 taaaaatgaa ttattattat ttaatctcga tatagccggt attgctgtta gcggtggaag
      901 tgcctgtagt agcggtaccg acataggctc acatgtaatt gcacaattaa aacgcgcacc
      961 gggttctact gctgtgcgtt tttcattttc gaagtttaat acgattgagg aaatcgattt
     1021 tgcggttgat aaaatccgtg aaatgatgcc cgtggctgtg agttaattct tcaggatatt
     1081 tgtatcttaa cggttccaat aattaaaaat ggaactcgtt tatggctaac cgtcgtagtt
     1141 ttcttaaaaa atctgctgct ttattcagcg ttgctaacct gccacaattt gttcatagcg
     1201 aagagataaa tgcggctatt cagcgcgcgc aaaaatctac accggaaata cttgcagctg
     1261 atgaagattt ttgggcgaca ataaaatctg cttatacggc ttcgaataca attatcaatt
     1321 taaataatgg cggcgttagt ccttcaccta aagtagtgca ggacgccatg caacggtata
     1381 gtcagctcgc aaacgaagca ccgagttatt acatgtggca gattttggat aagggcagag
     1441 aaaatttacg cgcaaaattg gccaatcttg caggatgcga ccccaacgaa atcgccatta
     1501 accgcaatac cactgaagca ttggataatg tgatttttgg attggatttc gaaaaaggag
     1561 atgaggtttt attgacgaaa caagactatc caaacatgat taatgcatgg aagcaacgtg
     1621 aaaaacgcga tggcattgta ttaaaatgga tagaccttga attaccctct gacgataccg
     1681 attatttagt aaaaaaatat acggaacaga ttacctcaaa aactaaatta gtacaagtaa
     1741 atcatattat aaattggacc ggtcatatta atcccgtaaa agaaattgct gctgctgtgc
     1801 atgccaaagg tcctgaagtg atgattgacg gcgcacatac attcggacac tttgtttttt
     1861 cagttcctga actcggtggt gattattatg gcaccagttt gcataaatgg atgagtgcta
     1921 caattggctc cggcatgtta tggattaaaa aggaaaaaat tcacaaagtg tggccattaa
     1981 tgggtgcacc ggatacctta gacaaaaatg atatccgcaa atttgaaact ttaggaacac
     2041 gtaattttcc tattgaatat agtattgcaa cggctattga ttttcatcaa atgattggcc
     2101 cagaacgcaa agaggcacgt ttgcgatatt tacgcgatta ttgggtaaat aaatgcagtg
     2161 atattccaac atttaaatta aatacttcat taactaaatc atattcttgt gcaattgcca
     2221 actttagttt ggagggcatt gaaccgggag atatttcaac aaaattattt accgaccacg
     2281 gtgtacattg cgtgagtata gtttgggaaa atattaaagg tgtgcgtatt gcaccgcacg
     2341 tatataccag tttgtacgac ctggatatac tggtaaaagg cattcacaaa attgccggaa
     2401 aataattaca agcttgtcaa aaatgccatc gtatctacat ggtcggcata atcgtttaaa
     2461 ccagggtatt gcgtttgtcc aaacgctgta tttcccggtg ctgcagcaac aatacattgt
     2521 aaatccggta aaataatatc cagtttttcc tgtaagtcct gctcgctttt atatgtttca
     2581 caatataata ccgatagcgg agaaaacaat tcagtacttt ctaccaacat ataaaaatcg
     2641 ttggtcagat aattggtgct attcaataat aataatgcac gattgtaatc gtaattattt
     2701 ttaaacttgg tattatcggt aacatatcga tagtcttccc aatatttaaa taataattgc
     2761 ctgtcgaatt gttccggaaa aaatacctta ccgacattcc tgcatcctaa tccgtaataa
     2821 taaaaaacat ctttacctaa attatttaac tgctcttcgg tttcattgcc cgttaatatg
     2881 gcaactgaag ttctgttttt gcgaatgata tgtggatatt ttccaaaata atattcaaaa
     2941 tatcgagccg aattattact tcctgttgca ataaccgcat caaaattttc taacttatct
     3001 acaaattgaa tacgttgttc aaaatcggta tttattttca ctaaaacctg tttgaaccaa
     3061 gggaaaaaaa acatgtcctt tgaactcagt tttacttttg cgttgtgacc acttgccagc
     3121 acacaaatta aatcgtgcat accaacaaaa ggtacattgc ctgctaatac caatccaatg
     3181 gttttgggag cagccggatt agtgttgtat tgagcaatcc aggtaaccaa agtatcggca
     3241 gctaataact tattacacat actatcgagc atgcgcaagc tttccagttg ggtaaaccat
     3301 gggttgttga tttcggtgcg atgtaatacc gccatcaaat cgtccggttt agtttgaata
     3361 aaagctccgg ctgctataag cccttcaata gtttttccgc tttccatacc tgatttcgtc
     3421 actttgccgc aaaattacat caagtacaat ggttgcggga cttaaaatgc atttctttat
     3481 ttagaatgat tataaattta gcttatatta acgtcctatt ttgaaatatc ctgacactgc
     3541 ttatatattt gggcactcaa aaaaagaaag aaaaaaatta tggcaattca tattacttcc
     3601 gactgtatta actgcggtgc ttgtgagccc gaatgtccga acaatgcgat ttatgaaggt
     3661 ggtgtagaat gggcgtttgc cgatggaacc actgttaaag gcgctgtaac catgattgat
     3721 ggttcaacaa tggacgctga tcaaaaaaat gcacctattg cagacgatat ttattacatc
     3781 gttcctgata aatgcaccga gtgtcagggt ttccatgagg aacctcaatg tgcggctgtt
     3841 tgtccggttg actgttgttt acctgaccct gatcatgtgg aatcagaaga acagttgttg
     3901 gcgaaaaaat caaaaatgca tatttgatac ccacaatatt ttctcagtgc gtcattccaa
     3961 cgaatggcgc attttttttt accccatgtc gatttgttta caaattgcat actgcaggtt
     4021 ttagcttttc cgttaatttg ccacaaa
//
LOCUS       DAORKW010000330         4038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1098_length_4038_cov_4.166457, whole genome shotgun sequence.
ACCESSION   DAORKW010000330 DAORKW010000000
VERSION     DAORKW010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1098_length_4038_cov_4.166457"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PLB46_11900"
     CDS             <1..1033
                     /locus_tag="PLB46_11900"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRF77269.1"
                     /translation="MKYSWKVSYPINNYNVSLNIGKYVHFSDVYTQTNGKDLALDYYV
                     LDYNLEVAKKQFEQVKPMMACYEKYLGPYPFYKDGFAMVETPYLGMEHQSVIAYGNKY
                     QTGYLGFDYSGIGLDFDYIIIHETGHEWWGNNVSMKDLADMWIHEGFCTYSESIYVEC
                     LYGNETALDYINASKRSISNDRPIIGLYNVNSEGSGDMYQKGAVFLNTLRYQVNNDPL
                     WWNIILGIQKDFAKQTVTTAQIENYISRKAGKDFSKLFDQYLRHANIPVLEYKFQRTS
                     AGLELNFRWVADVAGFDMPIRYISKGGKPVWVNPTTEWQLLNIEGLDEKTFKWDTRHF
                     YFTEKLVKS"
     gene            1139..1630
                     /locus_tag="PLB46_11905"
     CDS             1139..1630
                     /locus_tag="PLB46_11905"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRF77270.1"
                     /translation="MLNQLLEMAQNHLDGISRDIPELTDVDSKQITSITSKTVVDTIL
                     QQAKKGNMGSLREMLSGADTDNQSDVLQQLKGPVVNQLQSKLNISGQSAQQLAVMALP
                     IIMNMLNGKVNAAKTNGFDINNALQGLSQNKGGFLNSILGMFGGNNQNSKMINNIITQ
                     LIK"
     gene            complement(1682..2701)
                     /locus_tag="PLB46_11910"
     CDS             complement(1682..2701)
                     /locus_tag="PLB46_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HRF77271.1"
                     /translation="MIDLRSDTVTQPSAAMRSFMMQAKVGDDVFGEDPSINELEAKVC
                     ALFGTEAALFTPTATMSNQIALKILTHPLDEIICDRTSHIYNYEVGGYAFHSGCSIRY
                     LSGDRGIFTAEDVLRNINPEDVHKPITRLVSLENTSNRGGGKVYPVDVIQSIKQVCNE
                     NQLLLHLDGSRLMNAIVASGTSPKIYGELFDTITICFSKGLGCPMGSILTGKKVHLEK
                     ARRVRKVFGGGMRQAGIMAAACIYSLDNNVNRLEEDHQHAKALGACLSNLDYVQEILS
                     VETNIVIFRLADGIVDTHFIQHLASHNIHAFAIGDNWVRFVTHLDVDTTQINEVVEVL
                     KKYHA"
     gene            complement(2698..3336)
                     /locus_tag="PLB46_11915"
     CDS             complement(2698..3336)
                     /locus_tag="PLB46_11915"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRF77272.1"
                     /translation="MENFSLTLLIIILTCITSFMAFNNAALRGSALFYPFAMANRGEW
                     YRFLTSGFIHADFLHLGVNMYVLYSFGLILEKMYLPLYFGDTANLVYVGIYFLGMIVA
                     DIPSYIKHRRDATYRGLGASGAVAAVLFATILIGPFNGEIGLLFIPGLGIPPIVFGIL
                     YLFYSAYMGRAGRDNVNHDAHFYGAVFGFIFPGIFKPELFLFLIEQIKLQLR"
     gene            complement(3336..3797)
                     /locus_tag="PLB46_11920"
     CDS             complement(3336..3797)
                     /locus_tag="PLB46_11920"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRF77273.1"
                     /translation="MQVTFLSTGKTTDKLIAEQCATYVKRIIKYTTFSWKETPDIKSL
                     PPNEAKIKEGELIKKHLSKGDWVVLLDETGKEFTSQTFAQYITKKQLANIKHIVFVLG
                     GAHGFSEEIYALANEQLSLSKMTFPHQMVRLIFLEQLYRAYTIINNEPYHH"
     gene            complement(3843..>4038)
                     /locus_tag="PLB46_11925"
     CDS             complement(3843..>4038)
                     /locus_tag="PLB46_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77274.1"
                     /translation="TESYRFGNGFPGTKCFQSGDVYYYYDTPNTRVIFYVHGPKNWFQ
                     RNEAVIEMIAQSIVVGEQGF"
BASE COUNT         1366 a          744 c          702 g         1226 t
ORIGIN      
        1 aatgaaatat agctggaagg tgagttatcc gattaataat tataacgttt cgctaaatat
       61 tggcaaatat gtccatttca gcgatgttta tacccaaaca aatggcaaag atttggcact
      121 ggattattac gttttagatt ataatcttga agtagctaaa aaacagtttg aacaggttaa
      181 accaatgatg gcttgttatg aaaaatattt aggcccatac ccattttata aagacggttt
      241 cgctatggtg gaaacgccct atctaggcat ggaacaccaa agtgtaattg cttatggaaa
      301 taaatatcag acaggctatt tgggatttga ttattcagga attggtttgg atttcgatta
      361 tatcatcatc catgaaaccg gtcatgaatg gtggggaaat aatgtcagta tgaaagattt
      421 ggccgacatg tggatacatg aaggcttttg cacttattct gaaagtattt atgtggaatg
      481 tttatatgga aatgaaaccg ctctagatta tataaatgct tcaaaacgca gtatcagcaa
      541 cgacaggcct atcattggtt tatataatgt aaatagcgaa ggtagtggcg atatgtatca
      601 aaaaggggct gtttttctga acacactgcg ttatcaggtt aacaatgacc cactttggtg
      661 gaatattatt ttgggtattc aaaaagattt tgccaaacaa acagtaacta ctgcccaaat
      721 cgaaaattat atcagccgaa aagccggcaa ggattttagc aaattgtttg atcaatacct
      781 gcgacatgcc aatattcctg ttttggaata caaatttcag cgtacttccg ctgggttaga
      841 attgaatttc cgctgggttg cagatgtggc cggatttgat atgcctattc ggtatatcag
      901 caaaggtggc aaaccggttt gggtaaaccc tacaacggaa tggcaactac taaatattga
      961 aggactagac gaaaaaacct ttaagtggga cacccgccat ttttatttta ccgaaaaact
     1021 ggtaaaaagc tgaaattcaa taactgattg atttaccgct atttagtagc cacaaaagaa
     1081 aacgggatta ttgatattcc gttttttttt atataaattt accaaaaaca acccaggtat
     1141 gctcaatcag ttattagaaa tggcccagaa ccacttagat ggaatatcgc gtgatattcc
     1201 tgaacttact gacgtggata gtaaacaaat tacctccatt acctccaaaa cggtagtaga
     1261 taccattttg cagcaagcta aaaagggaaa catgggttcc ttgcgtgaaa tgttatccgg
     1321 cgcagatacc gataaccaaa gtgatgtgtt acaacaatta aaaggtcctg tcgtaaatca
     1381 attacaaagt aaactcaaca tttcaggtca aagcgcacaa cagcttgctg ttatggcatt
     1441 gcctataatc atgaatatgc taaatggcaa agtaaatgct gctaaaacaa atggttttga
     1501 tattaataat gcgctacagg gattatctca aaataaaggt ggatttttaa atagcatttt
     1561 aggtatgttt ggtggcaata atcaaaacag taaaatgatt aacaatatta ttacgcaact
     1621 tatcaaataa ccttattttt tatatttgca gagcaccgca cacattgtgt aggtgctttt
     1681 tttatgcgtg atattttttt aaaacttcaa caacctcatt aatttgcgta gtatcaacat
     1741 ctaaatgcgt gacaaaacgt acccaattat caccaatggc aaatgcatgt atgttatgtg
     1801 atgctaaatg ttgaataaaa tgggtatcaa caattccatc agctaatcgg aatataacaa
     1861 tattggtttc tacacttaat atttcctgca cgtaatctaa attgcttaaa caggctccca
     1921 acgctttagc atgttgatga tcttcctcca gacgattaac attattatcc aaactataaa
     1981 tacaagctgc agccataata cctgcctgac gcatacctcc gccaaaaact tttctcacac
     2041 gtcgtgcttt ttctaaatgc acttttttac cggttaaaat ggaacccatc ggacaaccta
     2101 atcctttgct gaaacaaatg gtaatggtat caaataattc accataaatt tttggactag
     2161 tgcctgaagc aacaatggca ttcattaatc gtgaaccatc taaatgtaac aaaagttgat
     2221 tttcgttgca cacttgttta atggattgaa taacatcaac aggatatact ttaccgccac
     2281 ctcgattgga agtgttttct aaacttacta atcgggtaat aggtttgtgt acatcttcag
     2341 gattaatatt acgtaataca tcttcagcag taaaaattcc tcgatctccg gataaatatc
     2401 ggatagaaca tcctgaatga aatgcatatc caccaacttc ataattgtaa atatgtgaag
     2461 ttctgtcgca aattatttca tccaatggat gtgtcaatat ttttaatgct atctgattcg
     2521 acatggttgc cgttggtgta aataatgcgg cttctgtgcc aaataaagca caaactttag
     2581 cttctaattc attaatgctc gggtcttcgc caaaaacatc atcacccact ttggcctgca
     2641 tcataaaact gcgcatcgct gctgaaggtt gtgttaccgt atcacttcgt aaatcaatca
     2701 tcttaattgc aatttaattt gttcgattaa aaataaaaaa agttcgggtt taaaaatgcc
     2761 cggaaaaata aatccgaaaa cggcaccata aaagtgagcg tcgtgattta cattatctct
     2821 accggcacgt cccatatatg cagaataaaa taaataaaga ataccgaaaa cgatgggtgg
     2881 aatacctaat cctggtataa ataataatcc gatttcacca ttaaaagggc cgattaatat
     2941 ggtggcaaat aaaactgcag caacggcacc ggaagctccc aatcctcgat aagtggcgtc
     3001 acgcctatgt tttatatacg aaggaatatc ggctacaatc atacctaaaa aataaatacc
     3061 tacatatact aaattagcag tatcaccaaa atataatggc aaatacattt tttcaagaat
     3121 aagcccgaat gaatataaaa catacatgtt tacgccaaga tgcaaaaaat cggcatgaat
     3181 aaatccactg gttaaaaacc gataccattc acctctgttc gccattgcaa acggataaaa
     3241 taaagccgaa cctcttaacg ctgcattatt aaacgccata aatgacgtaa tacatgttaa
     3301 aataataatg agtaatgtaa gcgaaaaatt ttccattaat ggtgataagg ttcgttgtta
     3361 atgatggtat atgctctata taattgttct aaaaaaatta aacgcaccat ttgatgcgga
     3421 aatgtcattt tcgacaaact caattgctca ttggctaaag cataaatctc ttcactaaat
     3481 ccatgagcac ctcctaaaac aaaaacaata tgtttgatat tggcgagttg ttttttggtg
     3541 atgtattgcg caaaagtctg tgaagtaaat tcttttcctg tttcgtccaa taacacaacc
     3601 caatcgcctt tcgaaagatg tttcttgatg agttcacctt cttttatttt agcttcattc
     3661 ggtgggagcg atttaatatc aggtgtctct ttccagctaa acgtggtgta tttgataatg
     3721 cgtttaacat atgtagcaca ctgctccgca atcagcttat ctgttgtttt acctgtactt
     3781 aaaaaagtga cctgcatgat gctttaaaga tagggaaagc tattgagtgc tcacaatatt
     3841 aattaaaaac cctgttcacc aactacaata ctttgagcaa tcatttcaat aactgcttcg
     3901 ttgcgttgaa accagttttt aggaccatgc acataaaata ttacacgtgt atttggcgtg
     3961 tcataataat aatatacatc accactctga aaacacttag tacccggaaa tccgttgccg
     4021 aaccgatatg attctgtc
//
LOCUS       DAORKW010000331         4013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1115_length_4013_cov_4.417635, whole genome shotgun sequence.
ACCESSION   DAORKW010000331 DAORKW010000000
VERSION     DAORKW010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1115_length_4013_cov_4.417635"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PLB46_11930"
     CDS             complement(<1..1071)
                     /locus_tag="PLB46_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF77275.1"
                     /translation="MKKLLLGIFSFVFTTMVFGQSVTQKYWVYFTDKNDTQYSVDQPL
                     AYLSESAVQRRAKMGIAINYYDIPVNAEYVSTIKNLGANVIVESRWLNAVSVESNAEQ
                     LTAIQALPFVKNTTSVKRYAIIDDSGIPIDDNIMLRTTNYIESDYGGAYNQNHMIDID
                     FLHNLGYRGQGVKIAVLDGGFDGVNVGEGFTSLYNKNQIIETRNFPDNNENVFFSSTH
                     GSNVLSIMAVDKPGKYIGSAPDAQYYLFRTEVVESERAIEEDYWLQAAEYADFIGADI
                     INSSLGYTTFDTIIEDHTYADMDGNTTVITQAADRAAAVGILVCNSAGNEGDGSWFYI
                     GAPADGDSVFTIGAVDLNGNFAS"
     gene            complement(1089..2816)
                     /locus_tag="PLB46_11935"
     CDS             complement(1089..2816)
                     /locus_tag="PLB46_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF77276.1"
                     /translation="MIRNCLLLLIVSLFIQTSFAQIALSPEFSETISTTSSTAQKYWV
                     YFHVKDDVNLQKAHPENYLSQRAIERRNKIGIGIEFSDLPVSTKNIDKLNQIGATPVV
                     ISKWLNAVSVYLTESQMEQVVMWDEVANIQKVKKYRRADEAIIPYNDQVFYRTAGTSD
                     VTGMFGLSSNQIEQINLDTILDLGYTGNDMVIAVLDAGFFGVDTATLFQSFWDKGQIL
                     GYKNFPDHNNEVFNIYSGYHGAWVMSVIGGDVADAFSGSAPNADFYLFRTEVADSEYV
                     VEEDYWLAASEKADSVGADIINSSLGYTTFDDSLENHTYEDLDGNTAVVTKAGDMAAQ
                     KGILVCNSAGNEGANTWHYISMPGDGDSVFTIGGVDTNGIHVGFSGYGPTFDGRLKPN
                     VVALALGSAVLDPGGYIGFLAGTSFSSPLIAGACASLWGAFPEKTNMEIMSAVERSAH
                     LYLTPNDSMGYGIPNFGLAYEILLSETDTTTEDTVIVDTTTAIFDILPNPAGDFFSVF
                     ITTNENAPATIQFYDLAGQLIYQEKIYLSKSIANTFTIDTRKNFSQGMYLFSLTGDGF
                     KQSTVVFVE"
     gene            complement(2831..3595)
                     /locus_tag="PLB46_11940"
     CDS             complement(2831..3595)
                     /locus_tag="PLB46_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77277.1"
                     /translation="MVSNHLLQNISKYFLLVAITSMVFFACDETEPPETLTTPLQYFP
                     LEIGKFRVYNVDSINYHETIPNDTSSWQVKEIIVDTFYDLTNNLNYTIERYRRQTAAD
                     EWIIANVWSVMFDNGQLVKNENNLRFIKLVEPVTTGTTWSGNVYLGGLDDLPVAEECN
                     ELTFYEDWEYNYANVHTTYDIDLFNFDNTLKVIQQGDSNLIWYDYAEEIYAENVGLIQ
                     QDFYHYYTQDLTCPTCPWEQRVQCGYSVKMRILDFN"
     gene            complement(3607..>4013)
                     /locus_tag="PLB46_11945"
     CDS             complement(3607..>4013)
                     /locus_tag="PLB46_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRF77278.1"
                     /translation="AGNILGTHKGYPFYTIGQRKGLGIALGEPYFVTEIKPAENVVVL
                     GKEDTLLRNGMFVHHLNMQKYESVNAGHSSVTKIRYKNTGSESMLSLEGNDLMRVEFK
                     ENVSAITPGQSAVFYEGNDVIGGGHILSSYIR"
BASE COUNT         1270 a          834 c          601 g         1308 t
ORIGIN      
        1 actggcaaaa ttgccattta aatcgactgc gccaattgta aatacactat caccgtcggc
       61 aggtgcgcct atataaaacc aactgccatc gccttcattg cctgcactat tacaaaccaa
      121 aatacccact gctgctgctc tgtccgctgc ttgcgttata acggtggtat tgccatccat
      181 atccgcataa gtatggtctt cgataatggt atcaaaagtg gtataaccta aagaagaatt
      241 aataatatca gcaccaataa aatcagcata ctctgcagcc tgtaaccaat aatcttcctc
      301 aatagctctt tctgattcta ctacttctgt gcgaaataaa taatattgtg catcaggtgc
      361 actgcctata tatttgcctg gtttatctac agccattata ctcaacacat tgctgccatg
      421 tgtcgaacta aaaaacacat tttcattatt atccggaaaa ttgcgtgttt caattatctg
      481 atttttatta tacaaagaag taaatccttc accgacattt acaccatcga aaccaccatc
      541 taaaactgca atttttacac cttgtcctct gtaacccaga ttatgcaaaa aatcaatatc
      601 aatcatatga ttttgattat atgcaccacc ataatcagac tcaatataat tggttgtacg
      661 caacattata ttatcgtcga ttggaatacc tgaatcatcg ataatggcat atcgttttac
      721 gctcgttgtg ttttttacaa aaggtagtgc ttgtattgca gttaattgtt cagcattact
      781 ttcaacactt accgcattta accatctgct ttctacaata acatttgcac ctaaattttt
      841 tattgttgat acatattcag cattaacagg aatatcataa taattaatgg caattcccat
      901 ttttgctcta cgctgaacag cactttcgga taaatacgcc aatggctgat cgacagaata
      961 ttgtgtgtca tttttatccg taaaatatac ccaatatttt tgtgtaaccg attgaccaaa
     1021 caccatagtg gtgaaaacaa aactgaatat gcctaaaagt aattttttca tgttatttcc
     1081 ccccgatact attcaacaaa aacgacagta ctttgtttaa acccatcgcc tgttagtgaa
     1141 aataaataca taccctgact gaaatttttg cgtgtatcaa tggtgaatgt atttgctatt
     1201 gattttgaga gatatatttt ttcctgataa attaattgtc ctgctaaatc ataaaattgt
     1261 atagttgccg gtgcattttc gttagtggta ataaacacag aaaagaaatc acctgcaggg
     1321 tttggtaaaa tatcaaatat agcagtagtt gtatccacta taacagtatc ttcagtggtc
     1381 gtatctgttt cagataataa tatttcatat gccaatccga aatttggaat tccgtaaccc
     1441 atcgaatcgt taggagttaa atataaatgt gcactgcgtt caacagcact cataatttcc
     1501 atattagttt tttccggaaa tgcgccccat aaactggcac aggcaccggc tattaaagga
     1561 gaagaaaaac tggttcctgc taaaaatccg atatatccac ccgggtctaa aacagcagaa
     1621 cccaaagcta atgcaacaac gttcggttta agtcgcccat cgaaagttgg tccgtaacca
     1681 ctaaaaccaa catgaatgcc attggtatca acaccaccaa ttgtaaacac cgaatcgcca
     1741 tctccgggca ttgatatata atgccatgta ttagcacctt cgttacctgc actgttgcat
     1801 actaaaatac ctttttgtgc tgccatgtca ccggctttgg taactactgc agtattgccg
     1861 tctaaatctt cataagtatg attctcaagc gagtcatcaa atgtggtata acctagggat
     1921 gaattaataa tatcggcacc aacactatct gctttttctg aagcggctaa ccaataatct
     1981 tcttccacta catattcact gtctgcaact tctgttctga ataaataaaa atccgcattc
     2041 ggagctgaac ctgaaaatgc atctgcaaca tcaccaccaa ttacactcat tacccatgca
     2101 ccgtgatatc ctgaataaat attaaacact tcattattat gatcaggaaa atttttatat
     2161 cctaatattt gtcctttatc ccaaaaactc tgaaacaaag tagccgtatc aacaccaaaa
     2221 aaaccggcgt ctaatactgc aattaccata tcattcccgg tataacccaa atcaagaata
     2281 gtatcaagat taatttgttc tatttgatta gatgacaatc caaacatacc ggtaacatcg
     2341 cttgttcctg cagtacggta aaacacctgg tcattatagg gaattatagc ttcatcggca
     2401 cggcgatatt tttttacttt ttgaatattc gctacctcat cccacatcac cacttgttcc
     2461 atttgtgatt ctgttagata aacactcacc gcatttagcc attttgaaat tacaaccgga
     2521 gttgcaccta tttgatttaa tttatcgata ttttttgttg aaaccggtaa atcagaaaat
     2581 tcaatgccga tacctatttt atttctgcgt tctattgctc tttgggataa ataattttca
     2641 ggatgtgctt tttgcagatt tacatcatcc ttaacatgaa aatacaccca atatttttgt
     2701 gcagttgagg aagtagttga aattgtttca ctaaattcag gagacaaggc tatttgagca
     2761 aatgaagttt gaataaacaa actaacaatg aggaggagta aacaatttct aatcatatta
     2821 atattttcat ttaattaaaa tctagtatgc gcatttttac actgtatccg cattgcactc
     2881 gttgttccca cggacaggtt ggacaagtta aatcctgtgt gtaataatga taaaaatcct
     2941 gttgaattaa tcctacattt tcagcatata tttcttcagc ataatcatac caaatcaggt
     3001 tggaatcacc ttgctgtatt actttcagtg tattatcaaa attaaataaa tcgatatcat
     3061 aggttgtgtg aacatttgca taattatatt cccaatcctc ataaaaagtt agctcgttac
     3121 attcttctgc aaccggtaaa tcgtccaatc cacccaaata aacatttccg ctccaggttg
     3181 taccggtagt tacaggttca actaatttta taaaccgtaa attattttca tttttcacta
     3241 attgaccatt atcaaacatc acactccaca cattcgcaat tatccattca tctgcagcag
     3301 tttgtctgcg atatcgttca atggtataat ttaaattatt ggttaaatcg taaaaagtat
     3361 ctacaataat ttctttaacc tgccatgagc tggtgtcgtt tggaatagtc tcatggtaat
     3421 taattgaatc tacattatat acgcgaaact ttcctatttc aagcgggaag tattgtaatg
     3481 gagtagttaa agtttcaggt ggttcagttt catcacaggc aaaaaatacc atacttgtaa
     3541 tggcaacaag caaaaagtat ttactgatat tttgaagcaa gtgattgcta accatatttg
     3601 ttattattaa cggatatatg aactcaaaat atgcccacct cctattacat cgttgccttc
     3661 ataaaaaact gcactttgtc cgggggtgat ggcactcaca ttttctttaa attcaacccg
     3721 catcaaatca tttccttcca aagataacat actttctgag cctgtgtttt tgtatcggat
     3781 ttttgttaca ctactgtgac cggcattaac actttcatat ttttgcatat tgagatgatg
     3841 cacaaacatg ccgttgcgca gtaaggtatc ttctttaccc aacactacaa cattttcagc
     3901 cggtttaatt tccgttacga aataaggttc tcccaatgca atacccaggc ctttgcgctg
     3961 ccctatggta taaaaaggat atcctttatg cgtgcctaat atatttcctg ctt
//
LOCUS       DAORKW010000332         4009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1118_length_4009_cov_4.303237, whole genome shotgun sequence.
ACCESSION   DAORKW010000332 DAORKW010000000
VERSION     DAORKW010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1118_length_4009_cov_4.303237"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(447..1463)
                     /gene="murB"
                     /locus_tag="PLB46_11950"
     CDS             complement(447..1463)
                     /gene="murB"
                     /locus_tag="PLB46_11950"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRF77279.1"
                     /translation="MDIKHQISLKPFNTFGIDVFAENFVAISTLGDLNVAFQQQLFAH
                     KFLILGGGSNVLFAGDFPGTIFKNNLKGISLLTTNEDTVLIKAASGEIWHDLVMYCVQ
                     NNFGGVENLSLIPGTVGAAPMQNIGAYGIELIDVFESLTAFDVKDGTYRIFTKEECDF
                     GYRSSVFKTIYKDRYFITDVTLRLSKSPVLNISYGAIKDVLAEKNIIQPTIKDISDAV
                     IAIRKSKLPDPNILGNAGSFVKNPETPESFYLQLKTTFPEMPGYPTTAGFTKVPAGWL
                     IEQCGWTGKQVGNTGAHKNQALVLVNYGNATGAEIMQHAFTIQQSVQEKFGITIIPEV
                     NLIS"
     gene            1559..3085
                     /locus_tag="PLB46_11955"
     CDS             1559..3085
                     /locus_tag="PLB46_11955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77280.1"
                     /translation="MYNKLFNTTGAKVFSWAIGLLVCFIMLSAPANGQEYKLLYEGRD
                     MTFAIQDAEEIDSLYYFVQIDSIGTSGEDTLYYFNHQLQPGIDGDCPLIYNDTVFLGR
                     KAIKRNDEFGTYVFFNGVGDSIFIRTKIKNGDFWKLFIYPDGSYIKATVINHLDGGII
                     NGLMDSIFRIKLNAYTNLGISMPDVFPSETKFDISKHYGMQEFFDMRVFPLPGDGKQR
                     KMRGITAPDTGIVDITARNAFTFETGYEFHYQEITCPDVMNGANKHISSYKYFVLAAN
                     ADESGATYDFVRVQIDSFIYDASTEVQTIWDTITRSYTFSDYVFLDTAELNIFEWLEQ
                     GYSDWEVNDTVYAGVPHKFVYDLYQYDEDTKCLTNPENNTMPLQLYGQGLGPIYYVDS
                     TSTNDFYSLRMVYFKLGLQEWGTPYDFSYLDNPVTNLYQDNNFEVYPNPAQAFITLNL
                     PESVLLHSNYTIYNISGVVAQEGITDSKISMPLLPNGLYFIEIHTEQGNWKSRFIKQQ
                     "
     gene            complement(3090..3794)
                     /locus_tag="PLB46_11960"
     CDS             complement(3090..3794)
                     /locus_tag="PLB46_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77281.1"
                     /translation="MTLAAEAIKNYFFAFSDLHIGSYHNILFNSIAFYLGFVLFLVGF
                     IKKCIKQMDFVEWLFLGNLCIVVFWPSYQGLRFFIPVLPFYLYYGMLGLKLLPGLRIQ
                     KAVFTLLMVCVAISFVSFYTNADYKQSPYLLTGSESEDLFTYIKSSTPNDATIMAAKP
                     RAVCLMTGRKGIVFPDSEYEKQFSENVSQHQVNYVLINNYPGFPNYLTNENAETTFQF
                     KKVFSNYHWVLYQVNS"
BASE COUNT         1263 a          728 c          708 g         1310 t
ORIGIN      
        1 gtcaacccac gtgttgacta tcgttttatt tgaattaaaa tgaattcggt ttattgttag
       61 atttttaata cgatgttacc gtagccgtca acccacgtgt tgattattgt tttatttgaa
      121 ttaaaatgaa ttcggggtat tattagatgt ataatgcggt gttaccgctg ccgtcaaccc
      181 acgtgttgac tatcgtttta tttgaattaa aatgaattcg ggttattgtt agatttttat
      241 tgcgatgtta ccgttgccgt caacccaggt gttgattatc atattatttg aattaaaatg
      301 aattcggttt attgttagat tttaaattgc aacattggtt gccattattt cgtgtatcac
      361 cccatcaaaa aaattaattc acaccatcca tccatccacc ctccgcttta gcggccccca
      421 cattagctaa tttgctaatt aaccaattag ctaattaggt tcacttcggg tattatggta
      481 atgccaaatt tctcctgcac actttgttga atggtaaatg cgtgttgcat tatttctgca
      541 ccggttgcgt tgccataatt tactaaaacc aaagcctgat ttttgtgggc accggtattg
      601 ccgacctgct tgcctgtcca accgcattgt tcaattaacc aaccggctgg aaccttggtg
      661 aaacctgcag ttgtaggata tccgggcatt tccggaaatg tagtttttaa ttgcagataa
      721 aatgattccg gagtttcagg gtttttgaca aaactacctg cattacctaa aatgttaggg
      781 tcaggtaatt tactttttct gatggcaatt acggcatcac taatatcttt tattgttggc
      841 tgaattatat ttttctccgc caaaacatct ttaattgccc cataacttat attgagtacc
      901 ggagatttac tcaaacgtaa agtaacatcc gtaataaagt atctgtcctt ataaattgtt
      961 ttaaatacac tgcttctata tccaaaatca cattcctctt ttgtaaaaat tcggtaggta
     1021 ccatctttga catcaaatgc agttagcgac tcaaaaacat cgattaactc aattccataa
     1081 gcaccgatat tttgcatggg agctgcacca actgtgcctg gtattaacga caaattttct
     1141 acgccgccaa aattattttg cacacaatac attactaagt catgccatat ttcaccggaa
     1201 gccgccttaa tgagtacagt gtcttcatta gttgttaata aggatatgcc tttgaggtta
     1261 tttttaaaga tggtacctgg aaaatcaccc gcgaacaaca cgttactacc accacctaaa
     1321 atcaggaatt tgtgtgcaaa caactgctgt tgaaatgcaa cgtttaaatc acccaaagtg
     1381 ctgattgcta caaagttttc ggcaaataca tctataccga aggtgttgaa tggtttgagt
     1441 gatatttgat gtttgatgtc caatttctta cccttttatg acttgtttta agtgccttag
     1501 cacccaatta agtgaaactt tacctatttt tacggtaaat gtaaggtaaa gaaatactat
     1561 gtacaacaaa ctttttaaca ccacaggagc aaaagtgttt tcatgggcaa tcggattgct
     1621 ggtttgcttt ataatgcttt cagcaccggc aaatggtcag gaatacaaat tgttgtacga
     1681 ggggcgggat atgaccttcg ccattcagga tgcggaagaa attgacagtt tgtattattt
     1741 cgtgcaaatt gattctattg gaaccagtgg tgaagacacc ttatattatt ttaatcacca
     1801 gttacaacct ggtattgatg gcgattgccc tttgatttat aacgataccg tttttttagg
     1861 tcgcaaagca ataaaacgca atgacgaatt tggcacttat gtgtttttta atggcgtagg
     1921 agatagtatt tttattcgca ccaaaataaa aaacggcgac ttctggaaat tatttattta
     1981 tccagatggc agttatatta aagcaaccgt aattaaccat ttagatggcg gaatcattaa
     2041 tggcttaatg gattctattt tcagaattaa attaaatgca tataccaatt taggtatttc
     2101 aatgcctgat gtatttccga gtgaaactaa gtttgacata tcaaaacatt atggcatgca
     2161 ggaatttttt gacatgcgtg tgtttccttt gcccggcgat ggcaaacaaa gaaaaatgcg
     2221 cggcataaca gctcctgata ccggtattgt tgatattacc gctcgcaatg catttacatt
     2281 tgaaaccggt tatgaatttc attatcaaga aataacctgc cctgatgtta tgaatggcgc
     2341 aaacaaacat attagttctt ataagtactt tgttttagct gctaatgctg atgaatctgg
     2401 agcaacttat gattttgtgc gtgttcagat tgattcattt atttatgatg catcaacaga
     2461 agtgcaaact atttgggata ccataacgcg ctcttatacg tttagcgatt acgttttttt
     2521 agatactgct gaattaaaca tttttgaatg gctggaacag ggttattcag attgggaagt
     2581 aaatgatact gtttatgcag gtgtgccaca taaatttgtt tacgatttgt atcaatatga
     2641 tgaagacact aaatgtttga caaacccaga aaataatact atgccattac aattgtatgg
     2701 gcaagggttg ggaccaattt attatgttga ttcaacttct accaacgact tttatagtct
     2761 gcgtatggtt tatttcaaat taggattaca agaatggggt acgccatacg actttagtta
     2821 tttagataat cctgttacga acttatatca ggacaacaat tttgaggttt atccaaatcc
     2881 tgcacaagca tttatcacgc ttaatctacc cgaatcggtg cttttacata gcaactatac
     2941 tatttataac atttcaggcg tagtagcaca agaaggtatt accgatagca agatttcgat
     3001 gccgctttta ccaaacgggt tgtattttat cgaaattcac actgaacaag gtaactggaa
     3061 atcaagattt attaaacagc aataaaattt taagaattaa cctggtataa tacccaatga
     3121 taattgctga acactttttt aaattgaaag gttgtctcgg cattttcatt tgttaagtaa
     3181 ttaggaaacc ctggataatt atttatcagc acataattaa cttgatgttg gcttacgttt
     3241 tcactgaact gtttttcata ctcgctgtca ggaaaaacta ttcccttgcg ccctgtcatt
     3301 aagcacactg ctcttggttt tgctgccata attgtcgcat cgtttggggt tgatgatttg
     3361 atataagtaa ataaatcttc actctcgcta ccggttagta aatacggact ttgtttataa
     3421 tctgcatttg tataaaagga aacaaatgat atggcaacac ataccatcag caatgtgaat
     3481 actgcttttt gtattcgcaa acccggcaat aattttaatc caagcatgcc atagtataaa
     3541 taaaatggta ataccggaat aaaaaaccta agcccctgat aagaaggcca aaatactaca
     3601 atacataaat ttcctaaaaa caaccattca acaaaatcca tttgtttaat gcactttttg
     3661 ataaacccga ccaaaaacaa aacaaacccg agataaaatg ctatggaatt aaacaggatg
     3721 ttgtgataag acccgatatg taaatcgcta aaagcaaaaa aataattttt gattgcctca
     3781 gcagccaatg tcataatttc cgtaaatgac atctgttggt aaacgtgaaa aacaatattc
     3841 agataactgt tatgctgaat aaaaatgatg gattgcagcc aggccaaaaa acaaaaaaac
     3901 ggaatgagca gtaacacgct tttccattta tcgaacctgt taatgatggc aaataaaatt
     3961 accgcaggca gcacaacaat accggctgtt cttatagcct acgtaaaat
//
LOCUS       DAORKW010000333         4004 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1122_length_4004_cov_3.642694, whole genome shotgun sequence.
ACCESSION   DAORKW010000333 DAORKW010000000
VERSION     DAORKW010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1122_length_4004_cov_3.642694"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="PLB46_11965"
     CDS             <1..1704
                     /locus_tag="PLB46_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HRF77282.1"
                     /translation="AQGRYPKSNNSANLNYRINRFNFYTNLGYNLHNTFQDLDINRTY
                     NNEDGSLSSTFYQHTFIKIENTSLTGKLGMDFYINDKSTIGVSFSGFNTSLDNNSTNT
                     AEVSDSLGGLQSTVVTYSPSVRKFINGNINLNYDVVLDDKGKQLTLQFDYLGYNSDMD
                     QQLLTKSYLPGDVLIGESNLLSFLPTLLSIKSAKADFVMPLSSGARFDAGVKSSIVDA
                     DNDADFQDELDGVLTPNYTFSNHFIYNENINAAYVNYSQSIGVFSLQLGLRFENTNIT
                     GNQLGNALTEDSTFTRNLYNIFPTAYLQYQVDSVGKHVLGFSYGRRIDRPNYKDLNPF
                     TYPLDLFTLYEGNPFLEPTFSHNFELSHTYNNILTTTGLFSVVNDVINETIEQRDGIF
                     YSRPGNISDQLTYGISVNGMFAIKKWWTLQVYSELMHNAFNATIYGQPLNNEGTHWYI
                     GPVNQFQVKETWAFELSGHYQTKVYSGQFITIPSGGVRFGVSKKIMKNQGTIKLNVSD
                     IFYTTQSGGDIEGLGNSTANWFSYLDTRVLTISFGYRFNKGQGMQARNTAAPDEKSRV
                     Q"
     gene            complement(1870..3801)
                     /locus_tag="PLB46_11970"
     CDS             complement(1870..3801)
                     /locus_tag="PLB46_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRF77283.1"
                     /translation="MDSINQETLTWTLWIVGVLVCIIFYKFILRFFFGMVIVPEDRIG
                     LVTKKFVLFGANKELPDGHIIATKGEAGYQAHSLAPGLYFWKWIWQYEITMQPFIIIP
                     TGQIGLVLANDGVELETGRILARKVECDTFQDAAAFMNGGGRKGRQSALIPPGSYRIN
                     TFLFAIEIVDMTNVPENGVGIVTTLEGQPLDEGQIAGKIIPSHNRYQDADTFLNNSGY
                     KGLQEQVILAGSYFLNPWFVRVEIVKMTEIPIGYVGVVISYVGADGVDLSGTEFKHGN
                     IVAKGQKGVWAEALGPGKYPINPYIMKVELVPTTNLVLNWASARTESHMLDKNLSTIT
                     VRSKDGFTFNLDVSQIIHIPTYEAPKVIARFGNMANLVTQVLEPTIGNYFRNSAQDAD
                     VIDFLKSRKERQESAKQHIGDVLEQYNVFGVDTLIGDIIPPETLMKTLTDRKLAEEQK
                     VTYDTQMKAQETRRTLEKETAVAEMQKELVKADQGVVIAEKIADASVKKATGDANGIR
                     IQSTAEGERLRILATGEAEKIRLLAEADSQKIKLIADAEAERIAKTGNAEAEKILAIG
                     KSNAESYKLSVEAMGGDNFTQLKITESLGMNNVKVIPEVLITGGNDSPNGPISGLLGL
                     ELMKELGKRIREGNQDKKE"
BASE COUNT         1196 a          758 c          740 g         1310 t
ORIGIN      
        1 gcgcaaggcc gttatcctaa aagtaacaac agcgcaaatc tgaattacag gataaacaga
       61 tttaattttt atacgaacct aggatataat ttgcataata catttcagga cttggatatc
      121 aacagaacgt ataataatga agatggtagt ttgagttcca ctttttatca gcatacattc
      181 attaaaattg agaataccag tctgaccggc aaattaggca tggattttta tatcaatgat
      241 aaatctacca ttggcgtttc gtttagtgga tttaatactt cgttggataa caattcaact
      301 aatacagcag aagtcagcga ttctttaggt gggttgcaga gtacagtggt tacttatagc
      361 ccctcagtcc gcaaatttat taatggtaat atcaatttaa attatgatgt tgtattggat
      421 gataaaggca aacaacttac acttcaattt gattacctgg ggtacaactc tgatatggac
      481 cagcaattac tcactaaatc atatttgccc ggtgatgtat taatcggaga aagtaacttg
      541 ctgagttttt taccaacttt attatccatc aaaagtgcga aagctgattt tgtgatgccg
      601 ctctcaagtg gtgcccgatt tgatgcaggt gttaaatcaa gtattgtgga tgcggataat
      661 gatgctgatt ttcaggatga attggatggt gttttaactc caaattatac tttttccaat
      721 cattttattt acaacgagaa tattaatgca gcttatgtaa attacagtca aagcattggt
      781 gtattttctt tgcaattagg attgcgattt gagaatacta atattacagg caatcaactt
      841 ggcaacgcac ttaccgaaga ttctactttt accagaaatt tatataatat ttttcctacc
      901 gcctatttac aatatcaggt agattcggtt gggaaacatg ttttaggatt ttcatatgga
      961 agaagaattg accgcccgaa ttataaggat ttaaatccgt ttacgtaccc gctcgatttg
     1021 tttactttgt atgaaggtaa tccatttttg gaacccactt tttcgcataa tttcgaatta
     1081 tcacatactt ataataatat cttaactaca actggattat ttagtgtggt aaacgatgtt
     1141 attaatgaaa ccatagagca acgtgatggt attttttata gtcgccccgg aaatatcagc
     1201 gatcaactga catatggcat ttcagttaat gggatgtttg caatcaaaaa atggtggaca
     1261 ttacaggtat attccgaatt gatgcataat gcatttaatg cgactattta tgggcaacca
     1321 ttaaataatg aaggaacaca ttggtatata gggcctgtga atcagtttca ggtaaaggag
     1381 acatgggcgt ttgaattatc gggccattat cagacaaagg tatatagcgg gcaatttatt
     1441 accattccat cgggaggtgt gaggtttggg gtttcgaaaa aaataatgaa aaatcagggc
     1501 acgattaaat taaatgtgag tgatatattt tatactacac aatccggtgg tgatattgaa
     1561 gggttaggaa attctactgc aaattggttt agttatttgg atacacgtgt gttaaccatt
     1621 tcgtttggat atagatttaa taaaggacaa ggaatgcagg ctcgaaatac agcagcacct
     1681 gatgaaaaat cgagggtgca ataataaacg gaaatgaaat tggttactgc tgttttagca
     1741 gcgcagattt ttattgtttg cgtcggacag agttctggca cgatgctgca gaattggaca
     1801 ttttgcggag cataaaaaaa ggagattatt gatataataa cctcctttta aaattgttgt
     1861 gaatcagtat tattcttttt tatcctgatt gccttcgcga attcgtttac cgagttcttt
     1921 cattaattct aaacctaaca aaccacttat tggtccgttt ggagaatcgt ttcctccggt
     1981 aatgagtact tctggaatta ctttcacatt attcattccg aggctttcgg taatttttaa
     2041 ttgcgtgaaa ttatctccac ccatagcttc aaccgataat ttataggatt cagcatttga
     2101 tttaccaatc gccaatattt tttccgcctc ggcattacct gtttttgcaa tacgttcggc
     2161 ttctgcatcg gcaattaatt ttattttttg cgaatcagcc tctgctaata atcgaatttt
     2221 ttctgcttca ccggttgcta atattcttaa tcgttcacct tcagctgtag attgaatacg
     2281 aataccgttt gcatcacccg tagctttttt cacactcgca tctgcaattt tttctgcaat
     2341 tactacacct tggtcagctt ttactaattc tttttgcatt tcagcaacag cagtttcttt
     2401 ctctaaagtg cgtcgagttt cctgcgcctt catttgggta tcgtaggtta ctttttgctc
     2461 ctcagccaat ttacggtcag tgagcgtttt cattaacgtt tcaggaggaa taatatcacc
     2521 gattaaggta tcaacaccaa aaacattata ctgctcaagc acatcaccaa tatgttgttt
     2581 ggcactttcc tgacgttctt ttcgtgactt gaggaaatca attacatcgg cgtcttgcgc
     2641 agagttacgg aagtagttac caatggtagg ttctaaaacc tgcgttacca gattggccat
     2701 atttccaaaa cgtgcaataa cttttggtgc ctcgtaagtt ggaatatgaa taatttgaga
     2761 aacatcgagg ttgaaagtga aaccatcttt agaacgcact gtgatagtac tcaaattttt
     2821 atctaacata tgcgattcag ttcttgctga tgcccaattt aaaaccaggt tggtagttgg
     2881 caccaattcc actttcatga tataagggtt aatcggatat ttccccgggc ctaatgcttc
     2941 tgcccataca cctttttgcc ctttcgcaac gatattaccg tgtttaaatt cagtaccact
     3001 taaatctaca ccatcggcac ccacgtaact gattacaaca ccgacataac cgataggtat
     3061 ttcggtcatt ttcacaattt caacacgcac aaaccatggg tttaagaaat aactaccagc
     3121 taaaattacc tgctcctgca aacctttgta accgctatta tttaaaaagg tatctgcatc
     3181 ctggtatcgg ttatgtgaag gaataatttt accggcaatt tgaccttcat ccaagggttg
     3241 tccctccaaa gttgtcacaa tacccacccc attttcaggc acattggtca tgtccacaat
     3301 ctctatagca aacaaaaagg tattgatacg atatgaaccc ggcggtataa gtgcagattg
     3361 ccggcctttt ctaccacctc cattcataaa agctgcggca tcctgaaaag tgtcgcattc
     3421 tacttttcgg gctaaaattc gtccagtttc cagttctaca ccatcatttg ctaacacaag
     3481 gccaatttga cctgtaggga tgatgataaa gggttgcata gtgatttcat attgccaaat
     3541 ccatttccag aaatataaac ccggtgctaa tgaatgtgcc tgataaccgg cttccccttt
     3601 tgtagcaata atgtgcccat caggtaattc tttgttggca ccgaataaca cgaatttttt
     3661 ggtgacaaga ccaattcggt cctcagggac tattaccatc ccgaaaaaga agcgcaaaat
     3721 gaatttgtag aagatgatac ataccagcac gcctacaatc cagagtgtcc aggtgagtgt
     3781 ttcttggtta attgagtcca ttgtgattgt tttaattgtt gaggaatatc cttaacagac
     3841 gacaaaataa actgcttatt ttagaatttg tgcattaact gtgaaatctt tttttattgt
     3901 gcccaaaacc atttgtacaa cacttaattt tgcaggaatt tttaaatcta cagcagtgaa
     3961 acttcgtact tcaactaacg agcaattcga acaacgccat attg
//
LOCUS       DAORKW010000334         4000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1127_length_4000_cov_4.024842, whole genome shotgun sequence.
ACCESSION   DAORKW010000334 DAORKW010000000
VERSION     DAORKW010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1127_length_4000_cov_4.024842"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLB46_11975"
     CDS             <1..391
                     /locus_tag="PLB46_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77284.1"
                     /translation="DSIATWAIDVSYPYVSLALINNEIFGIPFYSNVGGIALLEWNAV
                     PVVGIEATPITALQMYPNPVANTMHIMLSSGMKNYLIKDMSGRNILEGTTNNNNIDCT
                     SLNAGMYTINLFDTQGTILSSGVFIKL"
     gene            complement(467..2551)
                     /locus_tag="PLB46_11980"
     CDS             complement(467..2551)
                     /locus_tag="PLB46_11980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF77285.1"
                     /translation="MRKIFYLLAALGLTLHANAQNATAAKNQDLLRQVKIVNNQSYTV
                     HRNIIDATPIDNNVFYKQSSGARVGGDVVGYTSYDLVTNGCSPNHLLVYPDGKVSAVY
                     TGSTNTTDARPDRGTFYNTYDGSTWGSLPTSRIEDIRTGFPALINVGDHEMYFAHDGE
                     NNIVIYENESIGSNVWTENPNSLLLHGTWPRAAADPDNNYVHLLAANNDPSNTNNYML
                     YYRSSDNGATWDIQELRLPGIDTASGISVMGGECYVMRVIGNNVYIAAGESITDLSVW
                     KSTSNGDIGSWTRTRLIDFEFDNFDGNTISDVTGDGIADTLANHDGAIALAIDNDGMM
                     HVWTGVTRILDVEPDDDAWTYFPGTTGMWYWNETFGADSVQYLDFTLVDWDEDGDPFL
                     GIGANLPNYGCGFTSQPSATVDPETGYLYVVYTHPVEFTDYFGDPTVETAESFRDLFG
                     FYSTDNGVSWSPPINLSYTADLNFENVVPNVFWTTTNDKVHVMWMQDQEPGNSLENEA
                     PDPITTENAMLYRAFDYSRFEPYDPTAEYDYTGATNVLTFTNLSVDADAYSWNFGDGG
                     SSVIAEPTHVFAAVGTYLVCLNAENKYGEDQNCKTIEITQVTGIEEPTLDELITVYPT
                     LSAGAVTINISSFADDIQLSVININGQQIKTETITGEKTTIDLSDLAAGEYMLRFISR
                     SSVAIKQISLIK"
     gene            complement(2700..>4000)
                     /locus_tag="PLB46_11985"
     CDS             complement(2700..>4000)
                     /locus_tag="PLB46_11985"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF77286.1"
                     /translation="ILYGSSWTDLYLLTSTENGAEGTWTIQSIIETGFDNYQGDADQI
                     SDVTGDGIADTVETTDGFHEMILDDAGILHVWSGYYLLLDDDVAEGWSYFPSIYGMWY
                     WNSTMAMGEPTWIDLLVDWDDDGDPFLGIGADLGMYDGVTFTSMPAAAVDYSIGRIYL
                     TFTHPIEYTDLYEDPLNEAAQSFRDIFGTYSDDGGATWSAPINMTYTAHENQESTFPN
                     AYARIVGDCMHVLWMQDEDPGTSLDTNAPDAPGMSNMQYKCFDEARFNPYPPTAEYDY
                     TPDGVTGLVNFSNLSVDADSYSWDFGDGGSSTLENPAHVYATSNTYNVCLIATNKYDS
                     DNTCKTISINVAVVDYALDKALTVYPTPASSNVTVEVNGNFGTLFAEMYNALGEKMIS
                     TTSFNGSVNFDLSEVAAGNYIVKVFNQDGAFTSRQITVSK"
BASE COUNT         1305 a          784 c          691 g         1220 t
ORIGIN      
        1 agacagtatt gccacatggg ctatcgatgt gtcttatcca tatgtatcgt tggctttgat
       61 taataatgaa atttttggta ttccttttta ttctaatgtt ggtggaatag ccttattgga
      121 atggaatgca gtgcctgttg taggaattga agctacacca attacagcat tacaaatgta
      181 tcccaacccg gtagcgaata ccatgcatat tatgttatca agtggaatga aaaattattt
      241 aattaaggac atgagcggac gaaatatttt agaaggaaca accaataaca acaacataga
      301 ctgcacttcg ttaaatgcag gaatgtatac aatcaatttg tttgatactc aaggaacaat
      361 attgagttca ggcgttttta taaaactata aattcggaat tattggtgca aaaaaagatc
      421 ccgcattact gcgggattcc acatcaaaaa tgaataaagc accggtttat tttattaaac
      481 tgatttgctt tattgctact gagctacgag aaataaatct taacatgtat tcacctgctg
      541 ctaaatcgga taaatcgatt gtagtttttt caccggtgat ggtttcggtt ttaatttgtt
      601 gaccattgat attaattaca cttaactgaa tatcatcagc aaatgaagat atgttaattg
      661 taacagcacc cgcactcaaa gttggataca cggtaattaa ctcatccaat gttggttctt
      721 caataccggt tacttgtgta atttcaatgg ttttacaatt ttgatcttca ccatatttat
      781 tttctgcgtt taaacaaaca agataagtac ccactgccgc aaatacatgt gttggttcag
      841 caattactga acttccacca tctccaaaat tccagctata tgcatctgca tcaacactta
      901 aattagtaaa ggttaatacg tttgttgcac ctgtataatc atattcagca gttggatcat
      961 aaggttcaaa tcggctataa tcaaatgcac ggtataacat agcattttct gtcgtaattg
     1021 gatccggtgc ttcattttct aatgagttgc ccggttcctg gtcctgcatc cacatcacat
     1081 gtactttatc gttagttgta gtccaaaaaa catttggcac aacattttca aaatttaaat
     1141 cggcagtata ggataaatta attggaggcg accagcttac accattatca gtagaataaa
     1201 atccgaataa atctctgaat gattctgcag tttcaacggt tgggtcacca aagtaatcag
     1261 taaattctac agggtgtgta tataccacat ataaatatcc ggtttcggga tcaactgttg
     1321 cacttggttg tgaagtaaat ccacatccat aatttggtaa attagcacct attcctaaaa
     1381 atggatcacc atcctcatcc caatctacta aagtaaaatc taaatattga acactgtctg
     1441 caccgaatgt ctcattccag taccacattc ctgttgttcc cggaaaatag gtccatgcat
     1501 catcgtcagg ctctacatct aaaattctgg tgactcctgt ccaaacatgc atcattccat
     1561 cattatcaat agctaatgca attgcaccat catggttcgc taatgtatca gcaatgccat
     1621 cgcccgtaac atcggaaatt gtattaccat caaaattatc aaattcaaaa tcgattaaac
     1681 gtgttcttgt ccatgaacca atatcaccat tgctggtaga tttccaaacg gataaatcag
     1741 taatactttc accggcagcg atatatacat tattgccaat tacacgcatt acataacatt
     1801 caccacccat tactgaaata ccggaagcgg tatcaatacc cggtaaacgt aattcctgaa
     1861 tatcccaggt tgccccatta tcagaagagc gataatacaa catgtaatta ttggtatttg
     1921 acgggtcgtt attcgccgct aataaatgca catagttatt gtctggatca gcagcagcgc
     1981 gcggccatgt tccgtgtagt aataatgaat taggattttc agtccagaca ttggaaccaa
     2041 ttgattcatt ttcataaata acaatattgt tttctccatc atgagcaaaa tacatttcat
     2101 ggtcaccaac attaattaat gcaggaaaac ctgttctgat atcttcaatg cgtgaagtag
     2161 gtaatgatcc ccaagtgctt ccatcataag tattataaaa cgttccacgg tcaggtcttg
     2221 cgtcggttgt attagttgaa ccagtataaa cggcactcac tttgccatcg ggatatacca
     2281 ataaatgatt tggagaacaa ccattggtta ctaagtcgta cgatgtgtaa ccaacaacat
     2341 caccaccaac acgtgcaccg cttgactgct tataaaaaac attattatca ataggtgttg
     2401 catcaattat atttcgatgc acggtataac tttgattatt tactatttta acctgacgta
     2461 acaggtcttg atttttagcg gcagttgcat tttgagcatt tgcatgtaag gttaaaccca
     2521 atgcagcgag caagtaaaag atttttctca taacaattgt ttttagttat caattgctcg
     2581 caaagtattg caactttttg tctcagatgt atcaggtttg tcatttaaga tttcattaat
     2641 gttaattaac aaaaaacccc ggctattgcc ggggttttaa cttaaataat ttaagttaat
     2701 tatttgctaa cagtaatttg acggcttgta aaagcaccat cttgattaaa tactttaaca
     2761 atataattac cggcagcaac ttcagaaaga tcgaagttaa cgctaccgtt gaaagatgta
     2821 gttgaaatca ttttttcacc taatgcatta tacatttcag cgaataaagt accaaagttt
     2881 ccgttaactt ctacagttac gttagaagaa gcaggagttg ggtaaacagt taatgctttg
     2941 tctaaagcgt agtcaacaac agcaacgtta atgctgattg ttttacaagt gttatcgcta
     3001 tcatatttgt ttgtagcaat taaacataca ttgtatgtat tagaagtagc ataaacgtgt
     3061 gcagggtttt ctaaagttga agaaccaccg tcaccgaaat cccaagaata agaatcagca
     3121 tcaacagaaa ggttgctgaa gtttactaaa ccagtaacgc catcaggagt ataatcgtac
     3181 tcagctgttg gtggataagg gttaaagcga gcttcatcaa aacatttgta ttgcatatta
     3241 ctcataccag gagcatcagg agcattagta tctaatgaag tacctggatc ttcatcttgc
     3301 atccaaagca cgtgcataca gtcaccaact attcttgcat aagcattcgg gaaagttgat
     3361 tcctgatttt catgtgctgt ataagtcatg ttaataggag cagaccaagt agcaccacca
     3421 tcatcggaat aagttccgaa aatatcgcgg aaagactgag cagcttcgtt taatggatct
     3481 tcgtaaaggt cagtatattc aattgggtga gtaaaggtta aatagatacg accaattgaa
     3541 taatcaacag cagcagctgg catagaggtg aaagtaacac catcatacat accaaggtca
     3601 gcaccaatac ctaagaaagg atcaccatca tcatcccagt caactaataa gtcaatccac
     3661 gtaggttcgc ccatagccat agttgaattc cagtaccaca taccgtagat acttgggaaa
     3721 tagctccaac cttcagcaac atcgtcatct aataacagat aataacctga ccatacgtgt
     3781 aaaataccag catcgtcgag gatcatttca tggaaaccat ctgttgtttc aactgtatca
     3841 gcaataccat caccagttac gtctgaaatt tgatcagcat caccttgata gttatcaaaa
     3901 cctgtttcaa taatagactg aattgtccaa gttccttcag caccattttc ggtagaagtt
     3961 aaaaggtata aatctgtcca agagctaccg taaaggatat
//
LOCUS       DAORKW010000335         3984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1141_length_3984_cov_4.184780, whole genome shotgun sequence.
ACCESSION   DAORKW010000335 DAORKW010000000
VERSION     DAORKW010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1141_length_3984_cov_4.184780"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLB46_11990"
     CDS             <1..275
                     /locus_tag="PLB46_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77287.1"
                     /translation="DHNQSLSNASVGSILTHLDVYKIELNTDRFWLADELYIDNTFNV
                     ISQYLEKAQNLLDSDILNWYDENDQNKLNEYKKYFNDIKTSNNENK"
     gene            262..855
                     /locus_tag="PLB46_11995"
     CDS             262..855
                     /locus_tag="PLB46_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77288.1"
                     /translation="MKISKIYLLGLALVFLLFEANCKFQQPGNVIAKQIECTILVDSS
                     FVKLNHADAIDYTIFYAVQFNNNQESDLAIGMPCCNIESIIHFEQDIDFKSGKYKLFG
                     STGEDISDSILNLGKGQIAVVFAYRDSYVLIRNQSYSQLLNEIIDKHKHETLYYYLEN
                     KRSPIIKGADLFDKDKVHFVNEDSFNYNTFGNAYNSP"
     gene            1075..1455
                     /locus_tag="PLB46_12000"
     CDS             1075..1455
                     /locus_tag="PLB46_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77289.1"
                     /translation="MEDWGAYTSYLDSDTCILYINPFETKTTLEMSTFPLLDSNIIEK
                     PCYKGKYPIPNLFNKNEDIYFADIGLENMFEVYVIEANRDKKYQLFNMDENVQMPVNW
                     KNGYSFGFALNQTNNSIIYWLAMW"
     gene            1642..2712
                     /locus_tag="PLB46_12005"
     CDS             1642..2712
                     /locus_tag="PLB46_12005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRF77290.1"
                     /translation="MIPARKEEGLSQKKHDPFGMLLEGRNWEEGSEYRYGFNGKESDK
                     ETYGDGNVYDYGFRIYEPRIGRFLSVDPLAKNFPWYTPYQFAGNKPIAFLDLDGLEEI
                     WYGETNIINGNGFPVLEILFNTPEFADAIAQFRSSDNKGYDIIILEGRDLFTSDAAGT
                     YAEGQTFDFYTNEQILNETNANQHHSPQMQLHLNSIKPNVKNQIAAEGKGVIVIVLPD
                     LALQNSLRDMYDSKESRYVEAEKIAYTLGHEIVFHAVRSAVEGKKITEDPLNYDHRNI
                     FTDETNKRAWNSADQSPMVTVAKIAYPLSQGGKLYNGVEKSASELRAAGSDRPYERLP
                     QPIKPIKGSLPNTSNGVQVKSN"
     gene            2733..3578
                     /locus_tag="PLB46_12010"
     CDS             2733..3578
                     /locus_tag="PLB46_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77291.1"
                     /translation="MRIFIYCIVFTSLVRNTVIGQIIYPNTFDHSYKSKFSLNYDSIK
                     QINEFVYYCTDTALLSTNFVNLTIESEFKPMTEVLITNGVVSKVIHYNMNTTDTIHYL
                     YKASQLISSYHASAFGRISDSIYFRYHPYLIKQLKGGDTTEYCRIFEYDSSDVLVKEK
                     IYKNNETVAIIDYFFSDTLDITTFKFVPDTICFKQIVIKEYDNNLTFNEQIINRYKCI
                     GNGQASIQYFIYNEFNNVIEIQTYFNGELNGSEKYYYFLNYGLIFAVEEISQGLNKIT
                     KYVIN"
BASE COUNT         1468 a          567 c          667 g         1282 t
ORIGIN      
        1 atgatcataa tcaaagtttg agtaacgcat cagtagggtc tattttaaca cacttagatg
       61 tatataaaat tgaattaaat acagatcgct tttggttggc tgatgaactg tacattgata
      121 atacatttaa tgttatctct caatatttag aaaaagcgca aaatttatta gattcagata
      181 tcctaaattg gtatgatgaa aatgatcaaa acaaattaaa tgaatacaaa aaatatttta
      241 atgatattaa aacttcgaat aatgaaaata agtaaaatat atttgcttgg actggcttta
      301 gtttttcttt tatttgaagc aaattgcaaa tttcagcaac cgggaaatgt aatcgcaaaa
      361 cagattgaat gcactattct agttgattcc agttttgtta aacttaacca tgccgatgct
      421 attgattata caatcttcta tgcagttcag ttcaacaaca accaagaatc agacttagct
      481 attggtatgc cttgttgtaa tatagaaagt attattcatt ttgagcaaga tatagatttc
      541 aagtcgggga aatataaatt atttggatca acaggagagg atattagtga ttctatatta
      601 aacttaggta aaggacaaat tgctgtagtt tttgcatata gagatagtta tgtattaatt
      661 agaaaccagt cctatagtca gttactaaat gaaattattg acaagcataa acacgaaact
      721 ctttactatt atttagaaaa taaacgctca ccaattatta aaggtgcaga tttgtttgat
      781 aaagataaag ttcattttgt aaatgaggat tcatttaatt ataacacctt tgggaatgcc
      841 tacaactcac cttaaaaaac agtaattaat tgaattttgt attactcggc taatctagga
      901 ccagagattt agttaaaata caagattaaa tttactaaac cctatgacaa aaagaaaaag
      961 gcgcaagttc aacacggaat taaatgccaa ggtagcattg gccgccatca agaatcagaa
     1021 gacattagta tagctcagca aacagtatat cattaacgca gttacaattt ccaaatggaa
     1081 gactggggtg cttacacttc ctatttggat agtgacactt gcattttata tattaatcca
     1141 tttgaaacaa agactactct agaaatgtct acttttccac tattagattc aaatatcatt
     1201 gaaaagcctt gttacaaagg aaaatatccc atacctaatt tgtttaacaa aaatgaagat
     1261 atatattttg ctgatattgg acttgaaaat atgtttgagg tttatgtaat tgaagctaac
     1321 agagacaaga aataccagtt attcaacatg gatgaaaatg tccaaatgcc agtaaattgg
     1381 aaaaatggct actcttttgg ctttgcttta aatcaaacaa acaatagtat catttattgg
     1441 cttgcaatgt ggtaaaattt atatttattt cggaaaatct acaatgtaat tcattgacat
     1501 attttcaaaa ctgaaaaaaa agttaaataa attattacag taagaaccct taaagttaaa
     1561 ccagttgaaa ttgatgtctc aaatcgattg taaaaattac tcaaagtttc tactatttac
     1621 aaatttcagt tatatttaag catgattcct gccagaaagg aagagggttt gagccaaaaa
     1681 aaacacgacc cgttcgggat gttgttggaa ggaagaaatt gggaagaggg aagtgagtat
     1741 aggtacgggt ttaacgggaa agaaagtgat aaagaaacgt atggagatgg aaatgtttat
     1801 gattatgggt ttaggattta tgaacctaga attggtagat ttttaagcgt ggacccactc
     1861 gctaaaaatt ttccgtggta tacaccatat cagtttgctg gcaacaagcc cattgccttt
     1921 ttagatttag atgggttaga agaaatatgg tatggtgaaa caaatatcat aaatggaaat
     1981 ggatttccag tattagaaat attatttaat acaccagaat ttgcagatgc aatagctcaa
     2041 tttagaagct ccgataataa aggttatgat attataattt tagaaggtcg tgatttgttt
     2101 acaagtgatg ctgctggtac ttacgcagaa ggtcaaacat ttgattttta tactaacgaa
     2161 caaatattga atgaaacaaa tgccaatcag catcatagcc cacaaatgca actacacctg
     2221 aattcaataa agccaaatgt taagaatcaa attgctgctg aaggcaaagg tgtcattgtt
     2281 atagtcttac ctgacctagc attgcaaaat tcattaagag atatgtatga ttctaaagaa
     2341 agccgctatg ttgaagctga gaaaattgcc tataccttag gccatgaaat tgtttttcat
     2401 gcggtaaggt ctgcagttga agggaagaaa atcacagaag atccactaaa ttatgaccat
     2461 agaaacatat ttacggatga aacaaacaaa cgagcttgga attctgccga ccaatctcca
     2521 atggttacag tagctaaaat tgcataccct ttgtcacagg gagggaaatt gtacaatgga
     2581 gttgaaaaaa gtgcttcaga attacgagct gcaggttctg atcgaccata tgaaagatta
     2641 cctcagccaa taaaaccaat aaaaggtagt ttgcctaata catcaaatgg tgttcaagtt
     2701 aaatctaatt aacgcttaat aatttaattt ctatgcgtat ttttatttat tgtatagttt
     2761 ttacttcttt ggttcggaac acagtaattg ggcaaataat ttacccaaat acttttgatc
     2821 acagctataa gagtaagttt tctcttaact atgatagtat aaaacaaata aatgaatttg
     2881 tttattactg cactgacact gcactgctat caactaattt tgtaaattta accattgaaa
     2941 gtgagttcaa gccaatgact gaagttttaa ttacaaatgg agtagtttct aaagttattc
     3001 actacaacat gaatacaact gatacaattc attatttgta taaagcctca caactaattt
     3061 caagttatca tgcatctgca tttgggagga taagtgattc catttacttt cgttatcatc
     3121 catatcttat caagcaacta aaaggtggag atacaacaga gtattgtcgt atttttgaat
     3181 atgactcttc ggatgttttg gtaaaagaaa agatttataa gaataacgaa acagttgcaa
     3241 ttattgatta ttttttttcg gatacgttag atatcactac cttcaaattt gtaccggata
     3301 ctatttgctt taagcaaatt gttattaagg aatacgataa taatttgact tttaatgagc
     3361 aaattataaa tcgttataag tgcatcggaa atgggcaagc atcgattcaa tattttattt
     3421 ataatgaatt taataacgta attgaaatac aaacttattt taatggcgaa ttaaatggct
     3481 cggaaaaata ctattatttt ttaaactacg ggctcatatt tgcagttgaa gaaatatcgc
     3541 aagggctaaa taaaattaca aaatatgtta taaattagct tttcctaatg ttctaaattt
     3601 tgatgcctac ttgatggtaa tcatctaaat gaattaataa aatatttatt aatttgattt
     3661 tattctttaa tttgaatgac aaattgcaat tttacaaagc attaatatat taaattcaca
     3721 cggtagacga ccatcacttg tttaggatgc cactattaag tacaagttcg gaggtaagaa
     3781 attgtatgcc cccaaaatgt aaacactcga aaatttgaaa agttgacact atcgtccgaa
     3841 atcaaataat cacacatact tctactttaa ctaattttaa gttactttta aatatgctaa
     3901 ccaccagaat agacaagtat ttaggcgtta aagcacacga cccgttcgga atgttgttgg
     3961 aaggacgaaa ttgggaaggg ggaa
//
LOCUS       DAORKW010000336         3964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1151_length_3964_cov_4.801740, whole genome shotgun sequence.
ACCESSION   DAORKW010000336 DAORKW010000000
VERSION     DAORKW010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1151_length_3964_cov_4.801740"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PLB46_12015"
     CDS             complement(<1..380)
                     /locus_tag="PLB46_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77292.1"
                     /translation="MKIPVGTAVVILESGETGVIAYYDGNTAFIDVDGETVQAHAKDI
                     EPVASFGKAPAKATTTNTPKQFDSIEEGLLIQFAAVRNLSGELTRFDLSLVNNTPSNI
                     LFEYQFHVFDASPITIRKELNSAAT"
     gene            421..1071
                     /locus_tag="PLB46_12020"
     CDS             421..1071
                     /locus_tag="PLB46_12020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF77293.1"
                     /translation="MKKTIKWSLAQKAELKWWENYLKGKDVDGYHVWKRAYWQKLLDL
                     IAPVCPPQPGMDVLDAGCGPAGIFMNLAQCNVDAVDPLVDEYEKNLPHFKRSNYPYTT
                     FYSLPIEKYVANKQYDIIYCMNAINHVADINFSYDLLAKMVKPGGKLVVTIDAHNHGF
                     FKHLFRTIPGDILHPHQYDLKEYEGFLTERGFKMLKTDKLKHEFFFDHYMQVGERE"
     gene            complement(1452..2492)
                     /locus_tag="PLB46_12025"
     CDS             complement(1452..2492)
                     /locus_tag="PLB46_12025"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HRF77294.1"
                     /translation="MFNYCISSIKVQTKMLLLGGCLFFAGFNNFSVHAQNRADRSFSL
                     TEFVQPADSFNAKRLGLVLGTEATLYTGATIALYQYWYKDYPLTNFHLFNDNGEWLQY
                     DKMGHFYTTYFETNITTGFYKWTGMEDNKAYLAGALTSSVLQLTIEVFDGFSDKWGFS
                     IGDFTANTLGASLSAGQNFLWDEQRIKVKFSSHYEDYSGYDQTVQDRAADLFGTTGPE
                     KVLKDYNGQTTWLSVNPSMFMREDTRFPKWLMISGGYSVAGLLGGYENQWCTDPLMRP
                     EDCPPEDLIDYSNIDRYRQYYLSFDVDLTQIPTNSPYLKMLFEVLNIVKFPAPALEFN
                     QNHGVKFKPLYF"
     gene            complement(2533..3933)
                     /locus_tag="PLB46_12030"
     CDS             complement(2533..3933)
                     /locus_tag="PLB46_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77295.1"
                     /translation="MINIKFGTDGWRAIIAQEFTTDNVARVAKATADWINQISDKPAV
                     VIGHDCRFAGELFAEVTAKVCCEQGLTVYIAKGFVSTPMVSYGSFVLKTAAGVVITAS
                     HNPPAYNGFKLKSGYGGPTIPEDIALVESMIPETYVIPTTSLKEYEAQGKLHWVDLEQ
                     MYFDHVTKSFDLPAINNSGIKIGYDAMYGAGQRIAQRILTNMHVLHCDDNPGFKGQAP
                     EPIHRNLLELSDLIKYDPSLKCGLANDGDADRIGMYDGEGNFVDSHHIILLLIHYLHK
                     YKGYTGKVVIAFSVSEKVKRLCEHYNLDYEVTKIGFKYICGKMVVEDVLLGGEESGGI
                     AVKGHVPERDGIWCGLLLLEFMAKTGKTLTQLIDEVYAITGSFFFDRNDLHLTEELKT
                     TIINNCKNNTYTGFGNYTVQKMEDTDGFKFHLNDNSWVMIRASGTEPLLRVYAESPNQ
                     SETENILATVKKVLLG"
BASE COUNT         1216 a          715 c          768 g         1265 t
ORIGIN      
        1 gttgctgcac tatttaattc ttttctgata gtaattggtg atgcatcaaa tacatgaaac
       61 tgatattcaa acaaaatatt acttggtgta ttattcacca gcgataaatc gaaacgggtg
      121 agttcaccag ataaattgcg cacagcagca aattgtatca gcaaaccttc ttctatacta
      181 tcgaattgtt ttggcgtatt ggtagttgtt gcttttgccg gtgcttttcc aaaactggct
      241 accggttcaa tgtcttttgc atgtgcctgc acggtttcac catccacatc aatgaatgcc
      301 gtattaccat cgtaataagc aattacgcct gtttcgccac tttcgagaat tactactgct
      361 gttccaacag gtattttcat tgtttatgta attttacact ttaagcttac aaagttacga
      421 atgaaaaaaa cgataaagtg gagtctggct caaaaggctg aattaaaatg gtgggaaaac
      481 tacctgaaag gcaaagatgt agatggctac catgtttgga aacgcgctta ctggcaaaaa
      541 ttattggatt tgattgcacc tgtttgtccc ccacaaccag gtatggatgt actagacgca
      601 ggttgcggac cggcaggtat ttttatgaat ttagcacaat gtaatgttga tgctgttgac
      661 ccgttggtag atgaatatga aaaaaatctc ccgcatttta aacgcagtaa ttatccgtat
      721 actacatttt atagtttacc aattgaaaaa tatgttgcca ataaacaata cgatattatt
      781 tattgtatga atgcaattaa tcatgtggct gatattaatt tcagttatga tttattagca
      841 aaaatggtaa aaccgggtgg gaaattagtg gtgacaattg atgcacataa tcatggattt
      901 tttaaacatt tatttagaac aattcccggt gatattttac atccgcatca atatgatttg
      961 aaagagtatg aagggttttt aacggagaga ggatttaaaa tgttgaagac ggataagttg
     1021 aagcatgagt tcttttttga tcactatatg caggtggggg aacgtgaata aggggaacgc
     1081 ggaccctgcg gggcagggcg ctgattgggc ggattaacgc ggattttttg tgttgtgagt
     1141 gaatggttga gggaaatata ggttgggcct gcctggcggc ggatgggcgc ggattttttt
     1201 tatgatgtgg gtggatggaa atataggatt gatccggatt ttttgaaaag gatcgcagat
     1261 tgaacgggtt gaacggataa gggacggatt ttttgaaaag gaacacgggt tgaacgggtt
     1321 aaacggataa aaacggattt gttaggaggt ttaattgcgc aaggcaatta aaaaaaatgt
     1381 tgatttgaaa acggattgtg atatgtagca gtaacaccat tagctaattt gctaatttgc
     1441 taatttgcta attaaaaata cagcggtttg aatttgaccc cgtggttttg gttgaattcg
     1501 agggcggggg cagggaattt tacgatgttg aggacttcga ataacatttt gagatagggt
     1561 gagttggttg gaatttgagt gaggtctaca tcaaaggata aataatattg gcgataacgg
     1621 tcaatattgc tataatcgat taaatcttca ggagggcaat cttcggggcg cataagcggg
     1681 tcggtgcacc attggttttc gtaaccgcct aataaaccgg caacactata tccaccggaa
     1741 atcattaacc attttgggaa gcgggtatct tcacgcataa acatagaagg attaacactt
     1801 agccatgtag tttgtccatt gtaatctttt aatacttttt cgggaccggt tgttccgaat
     1861 aaatcggctg cacgatcctg cactgtttga tcataaccgg aataatcttc gtagtgtgat
     1921 gaaaatttta ctttaatacg ttgttcatcc cataaaaaat tttgacctgc acttaatgat
     1981 gcacctaaag tattagctgt aaaatcgccg atggaaaatc cccatttatc tgaaaaccca
     2041 tcaaatactt cgatagtcaa ttgcaaaaca gatgatgtta atgcaccagc taaatatgct
     2101 ttattatctt ccatgccggt ccatttgtaa aaaccggtgg tgatattcgt ttcgaaatac
     2161 gtggtgtaaa aatgccccat tttatcatat tgtaaccact caccattgtc gttaaataaa
     2221 tggaagttgg ttaagggata atctttatac caatattgat ataatgcgat agtagcacct
     2281 gtataaagtg tagcttctgt gccgagtacc aaacctaatc gttttgcatt aaacgaatcg
     2341 gcaggttgca caaattcggt caaactaaat gatctgtctg ctctgttttg tgcatgaaca
     2401 gaaaaattat tgaaacctgc aaaaaataaa catcccccca ataaaagcat tttagtttga
     2461 acttttattg aggagatgca ataattaaac atgatatgag tttgatgtaa aaataatttt
     2521 cagcgcaaca atttatccta acaacacttt tttaactgtt gccagaatat tttctgtttc
     2581 cgactgattt ggtgattcgg catatacacg taataaaggt tctgttccac tcgcgcgaat
     2641 cattacccag ctattatcat ttaaatggaa tttaaatcca tcagtatctt ccattttttg
     2701 aacggtataa tttccgaagc cggtgtaggt attattttta caattattaa taattgtcgt
     2761 ttttaattcc tctgttaaat gcaaatcatt tctgtcgaag aagaaactac cggttattgc
     2821 atatacttca tcaattaatt gcgtaagtgt ttttccggtt ttagccataa attcgagtaa
     2881 taacaatccg caccagatac catcgcgttc aggaacatgc ccttttacag cgataccgcc
     2941 actctcctcg cctcctaaca atacatcttc taccaccatt ttgccgcaaa tgtatttaaa
     3001 cccgatttta gttacttcgt aatccagatt ataatgttcg cacaaacgtt ttactttttc
     3061 gctcacgcta aatgcaatta ctactttacc ggtgtaccct ttgtatttat gtaaataatg
     3121 aattaataat aaaataatgt gatgtgaatc aacaaaatta ccttcaccat catacatacc
     3181 aattctgtct gcatcaccat cgttggctaa cccacatttt aacgatggat catatttaat
     3241 taaatcgctt aactccaata aatttctatg aataggttcc ggtgcctgac ctttaaagcc
     3301 gggattatcg tcgcaatgta acacatgcat attagttaaa atacgttgcg caatacgttg
     3361 tcctgcgccg tacatggcat cataacctat ttttatcccc gaattattta tagcaggtaa
     3421 atcaaaactt tttgttacat ggtcaaaata catttgttct aaatcaaccc aatgtaattt
     3481 accttgtgct tcgtattctt ttaatgatgt agttggaata acatacgttt caggaatcat
     3541 actttccact aacgcaatat cttcagggat agttggtccg ccataaccac tttttaattt
     3601 aaatccgttg taagcaggcg gattatgcga tgcggtaatt accacacctg ctgcagtttt
     3661 taatacaaaa gaaccataac tcaccattgg cgttgaaaca aaacccttgg caatataaac
     3721 agtgagacct tgttcgcagc aaacttttgc tgttacttca gcaaataatt ctccggcaaa
     3781 acgacaatcg tgaccaataa caacggcagg tttatctgaa atctgattaa tccaatcggc
     3841 agtggctttc gcaacacgtg caacattatc ggtagtaaat tcctgggcaa taatagctct
     3901 ccagccgtcg gttccaaatt taatatttat catactttac aactaaggtt ttgaagtgta
     3961 gccc
//
LOCUS       DAORKW010000337         3953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1159_length_3953_cov_4.603386, whole genome shotgun sequence.
ACCESSION   DAORKW010000337 DAORKW010000000
VERSION     DAORKW010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1159_length_3953_cov_4.603386"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1951
                     /locus_tag="PLB46_12035"
     CDS             <1..1951
                     /locus_tag="PLB46_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF77296.1"
                     /translation="SSSEYWILKIDSLGAIQWQNTIGGNGSDLPLSAYSNADGTCIVA
                     GYSSSGATGDKTEANLGGFDFWILKLSAAGGVLWQNTIGGNNSDIPNSVVPAIDDGII
                     IGGYSSSGISGDKTEALIGITDYWLLKLNTAGTIVWQNTIGGTADDYLFSVVPNIAAN
                     KYFLFGYSYSGIGGDKTEAGDPTANYWMLETNLSGTILGQETIQATGYDFGYAASICV
                     DGDYVVGGKSNSGIGGDKTENNNGDFDFWLVKMTTCEATTEVCNTLDDDCDGLIDEGV
                     KSIFYGDSDGDGFGVPEITILACTAPEGYADIIGDCYDANPAIYPGNTEICNGFDDNC
                     TGSVDEGLLITFYADIDGDLYGDPADFELLCEGIAGYVEDNTDCNDANNLINPGATEL
                     CNNIDDNCDGNIDEALDVTVTISASGSTTICQGSNVTLIATHNGDAVQWKKNGIDIIG
                     ATGVTLLVNATGTYTCMAYTNCDTTISSGIVVTVNKNPKAIISAGGPTEFCAGGNVTL
                     TETPSGGSTYQWYKGAAPIPGATATTYVATTTGNYKCRVTKTATGCYKISNPIAVLVT
                     CKEGEELTSNNISIYPNPTSDQLNISTDIIVSAIKIYNNSGAVVQQITNWQGESIDVS
                     QLASGVYYLQLFSETTTKQKIFVKE"
     gene            complement(2296..3516)
                     /locus_tag="PLB46_12040"
     CDS             complement(2296..3516)
                     /locus_tag="PLB46_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77297.1"
                     /translation="MEKLILGIAVVIAFNTSANAQINLIGAAVNDSTGKIDLVQWEAL
                     DSFSVVTTPTILDGYYFATSAFDAFNAKYYITGISGDSSGLYSFNSGTAEENLTEGSA
                     FSNVSEFDMSTGKMYNLIVETEEYINIYEFDVNTSEETLIGTIYEPGAIGIISDAIGF
                     DSNNGIIYYVGFTNDPALCLYAIPVRDTVFSFTKTILNTTGPYNNITCVNFDNVSEKI
                     FALNDTYDSLFNFAGRYIIEIDKVTGDIINRGDLAEFPYYVGGSSCFDQNTGTFMLAA
                     IDTSNVLKMVAFNTLTDTYVAGFIPSVVSEIVCDNSQFAKNKYSTTAINQELASNLNV
                     YPNPVTSVLSIEYTSSSPVLVQVSDSNGKQLITRNYNSTNKIDLNLESFAPGLYIVTI
                     TSGEQTVAQKIMVL"
     gene            complement(3767..>3953)
                     /locus_tag="PLB46_12045"
     CDS             complement(3767..>3953)
                     /locus_tag="PLB46_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77298.1"
                     /translation="NDITLILHMLMCFGLETGVASGVENSTTTGIVLHFLIAINIGII
                     FFELYFAILYYASDEVL"
BASE COUNT         1305 a          709 c          746 g         1193 t
ORIGIN      
        1 ctcatcttcg gaatattgga tattaaaaat tgatagttta ggtgccattc aatggcaaaa
       61 tactattggt ggaaacggaa gcgatcttcc tttatcagct tatagtaatg cagatggaac
      121 ttgcattgtt gccggatatt cgtcgagcgg tgcaacaggc gataagactg aagccaattt
      181 agggggtttc gatttttgga ttttaaaact cagcgctgca ggtggcgtgt tatggcaaaa
      241 tacaatagga ggaaataata gtgatattcc aaacagtgtt gtgcctgcaa ttgatgatgg
      301 tatcattatt ggtggttaca gtagctccgg aatttcaggt gataaaacag aagccttaat
      361 tggtattacc gattattggt tattaaaatt aaatacagca ggtactattg tttggcaaaa
      421 taccattggc ggaacagccg atgattattt atttagtgta gtgcctaata ttgcagcaaa
      481 taaatatttt ttattcggtt attcttattc cggaattggt ggtgataaaa cagaagccgg
      541 tgaccccact gcaaactatt ggatgctgga aacaaattta tccggaacta ttttaggaca
      601 ggaaaccatt caggcaacag gatatgattt tggttatgcg gcaagtatat gtgttgatgg
      661 tgattatgtt gttggtggta aatcaaatag tggaattggc ggcgataaaa cggaaaataa
      721 taatggtgat tttgattttt ggctagtaaa aatgacaacc tgcgaagcaa caaccgaagt
      781 ttgtaataca ctcgatgatg attgcgacgg attaattgat gaaggtgtaa aaagtatttt
      841 ttatggtgat tcagacggcg atggtttcgg cgttcctgaa attacaatat tggcatgcac
      901 agcacctgaa gggtatgcag atattattgg cgattgttat gatgcaaacc ctgctattta
      961 tccgggtaat actgaaatat gtaatgggtt tgatgacaat tgtaccggat cggtagacga
     1021 aggtttactc attacatttt atgccgatat tgatggtgat ttatatggtg atccagcaga
     1081 ctttgaatta ctttgtgaag gcattgcagg atatgttgaa gataatacag attgtaatga
     1141 tgctaataat ttaattaacc ccggcgcaac agaattatgt aataatatcg atgataattg
     1201 tgatggaaat attgatgaag ctttagatgt tacagttaca atttctgctt caggttcaac
     1261 aacaatttgt cagggaagta atgtcacatt aattgctacg cataatggtg atgcagtgca
     1321 atggaaaaag aatggcattg atataattgg tgcaacaggt gtcactttat tggtaaatgc
     1381 aacaggcact tatacctgta tggcatatac taattgcgat acaacaattt catctggtat
     1441 agttgtaacc gtaaataaaa accctaaagc aattatatct gccggcggcc caactgaatt
     1501 ttgtgcaggc ggtaatgtta cactaactga aacgccatct ggtggttcca cttatcagtg
     1561 gtataaaggt gcagcaccaa ttccgggggc aacagcaaca acttatgtag ccaccacaac
     1621 aggtaattat aaatgtcgcg tgacaaaaac agcaacaggc tgttataaaa tatccaatcc
     1681 aattgcagta ttagtcacct gtaaagaagg agaggagctg acatcgaata atatatccat
     1741 ttatccaaat ccaacttcag atcaattaaa tattagcaca gatattatag tttcagcaat
     1801 caaaatatat aataattccg gggctgttgt tcaacaaata actaattggc agggagaaag
     1861 tattgatgta tctcaattag cttcaggagt atattattta caactattta gtgaaacaac
     1921 aacaaaacaa aaaatatttg tgaaggaata aaatagcttg catatttcag cccaattaca
     1981 cctaacccgt agggttttcc gttgtcgtgg agtttttaac tccagacgcc accccggtct
     2041 ccagaccggt acaaattagt gatgagcaat gagtaatgag cacttccgag aaattgagga
     2101 cgttaatgtt atttatttat catttttcct agcctgaaca ttttaataac agccgccacc
     2161 accaagaaac aaaggccgca aagaaacacg aagattttat tacaatctac ttggcgttcc
     2221 ttagcggcct tcaggattat ggctgttaaa tgacgagatt aatgttgatt atttactctc
     2281 atcactcatc cctcatcaca acaccataat cttctgagca acggtttgtt ctccactagt
     2341 tatggttacg atataaagtc ccggtgcaaa tgattccaag tttaaatcaa ttttatttgt
     2401 ggaattataa ttgcgtgtga ttaattgttt accatttgaa tcagacacct gaaccaatac
     2461 agggctcgat gaagtatatt caatactcaa tactgatgta acaggatttg gatacacatt
     2521 taagttggaa gccaattcct gatttatagc tgttgtggaa tatttatttt tagcaaattg
     2581 cgaattatcg caaacaattt ctgaaacaac actaggaatg aaaccggcta cataagtgtc
     2641 agttaaagta ttaaaagcaa ccattttgag tacattacta gtatcaattg ctgctaacat
     2701 aaatgttccg gtattttgat caaaacatga tgaaccacca acgtaatacg ggaactcagc
     2761 aagatctccg cggttaataa tatcaccagt tactttatct atttcaataa tataacgtcc
     2821 tgcgaaatta aataacgaat cataagtatc gttcagcgcg aatatttttt cactaacgtt
     2881 atcgaaattt acacaggtaa tattgttata aggacctgtc gtatttaaaa ttgttttggt
     2941 aaatgaaaat accgtatctc taacaggaat agcatataaa cacaatgcag ggtcattggt
     3001 gaaacctaca taataaatta ttccattatt cgaatcaaaa cctattgcat cgctaattat
     3061 tccgattgca cccggttcat aaatagtacc aatcaaagtt tcttcgcttg tgttaacatc
     3121 aaattcgtaa atattaatat actcttcagt ttccactata agattataca ttttccctgt
     3181 actcatatca aattctgata cattagaaaa tgcagaacct tccgttaaat tttcttcagc
     3241 cgttcccgaa ttaaacgaat ataaacccga actatcgccc gaaattccgg tgatataata
     3301 tttggcgtta aatgcatcaa atgctgaagt ggcaaagtaa tacccgtcca aaatagttgg
     3361 tgttgtcacc acagaaaatg aatcaagtgc ctcccactga acaaggtcaa tttttcctgt
     3421 tgagtcgtta accgcagccc cgatgaggtt gatttgggcg ttagcgctgg tgttgaaggc
     3481 aattactact gcgatgccta gtataagttt ttccatgctt cgtttgtttc catacaaaca
     3541 ccggtaaaac ttgaatgttc aagttttaac tataatttcc acaaataccc ctaaaaccac
     3601 ctatttcagg tataaacctc ctttgaatat tcagtataat cgttgaaaag cgggctaaaa
     3661 aacagccgaa accacaatcc cgatatctat cgggacacta aggaaattgc acagagaacc
     3721 caaaaaaaca accctgctct gatgcatctc cgattcagca gcatctttac aggacttcgt
     3781 ccgacgcata atacaatatc gcaaaatata attcaaaaaa aataatgcca atattaatcg
     3841 caattaaaaa gtgtagcact atccccgttg tcgtggagtt ttcaactcca gacgccaccc
     3901 cggtctccag accgaaacac attagcatgt gtaaaattaa agttatatca ttt
//
LOCUS       DAORKW010000338         3946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1162_length_3946_cov_4.311231, whole genome shotgun sequence.
ACCESSION   DAORKW010000338 DAORKW010000000
VERSION     DAORKW010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1162_length_3946_cov_4.311231"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /gene="msrB"
                     /locus_tag="PLB46_12050"
     CDS             complement(<1..318)
                     /gene="msrB"
                     /locus_tag="PLB46_12050"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRF77299.1"
                     /translation="MKEKRQYTVIKSEDEWKSLLSEEQYRVLRKKGTERPFTGEYDHF
                     FEDGIYVCAGCGQQLFTSTSKFDSGCGWPAFDREIEKGTIDYETDQGFGMVRTEILCS
                     KCGG"
     gene            complement(514..1938)
                     /locus_tag="PLB46_12055"
     CDS             complement(514..1938)
                     /locus_tag="PLB46_12055"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HRF77300.1"
                     /translation="MKRVVFLLICSFLLSNLFGQTLHGYEFKIQIKGLADTALYLGHH
                     YGSSQYVIDTVPVNSKGEAVFTSNDTLPGGVYLVVIPTLKNKYFEMLVSGMEPKFSLE
                     TDTVDFIKNMRVSGSLENTIFYEDLNFIASKKDKMTQLKKELDLAGETTEAGVKIKAE
                     MKAINAEVEAYREANFNKYPNLLYTKFLKAATDIKIPEAPVNPDGTKDSTFALRYVRQ
                     HYFDNFDFSDERLLRSNLYDTRIKKYIKDYVVKMPDSIMVAVDFIIGKSTVSPKTFQY
                     VTVLLLNEYATSKIMGFDAVYVHIVDKFYTGGKAFWLDDVGLYRIQAQADKVRPTLLG
                     KKGQPLILQDTSGVDIPLYSINSRFTVIVFWSPDCGHCKKEMPKIEALYPELKQLGAE
                     VYAVYSEDEFDKWKTWLRGHKYPWINVSNSKGIDQYQIKYNVEQTPLIYLLDKDKIIF
                     GKKINIEQVPGIIKDRIKYEESKS"
     gene            complement(1943..3265)
                     /locus_tag="PLB46_12060"
     CDS             complement(1943..3265)
                     /locus_tag="PLB46_12060"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRF77301.1"
                     /translation="MMTYKETLDYLFTHLPMYQRIGAAAYKKDLTNTLALLQVLDNPQ
                     HTFTSIHVGGTNGKGSVSNMLAAVCAEAGYNTGLYTSPHLIDFRERIRVNGQLCEEHF
                     VITFVEKIKPHIDIIQPSFFEITVAMAFDYFRYKNVQIAIVEVGLGGRLDSTNVIQPL
                     VSVITNISFDHMQMLGNTLPLIAAEKAGIIKPQIPVVIGEIQDETIGVFEQKATENNA
                     ALYPAPENVQVELLDHSFDSMELNVSYLNQLTYPNLILDLTGDYQLQNVATAIQTIEV
                     MRLQGIEIPDEAIYEGLRDVQTHTGFAGRWQVLQKNPLLVADCAHNTGGLTLLFRQVK
                     EQSYQQLHVVTGAVNDKDLDAGLSLFPAHAIYYFCKPDIPRGLDAVHLQELALSKGLK
                     GTSYTSVFAALQQALQNAGKNDMILICGSIFVVAEALPAYTALTKLNS"
     gene            complement(3395..>3946)
                     /locus_tag="PLB46_12065"
     CDS             complement(3395..>3946)
                     /locus_tag="PLB46_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77302.1"
                     /translation="NQTVKEEIATQDIEQSVKIPDVKKNDKPKTNTNTQTETKPTIDQ
                     SQIFTGDKVNNNGQSGNSEGNSTYGGNQGSTNGDPNGYHGPGAGKGDDGNVGWDLAGR
                     SMKAKPSLQVEHNEIGDVRIKIYVDKNGKVTRAEYERSGSTITDSYLITQAKNAALKS
                     TFNADSKAPETQIGYITFHFKLQ"
BASE COUNT         1185 a          788 c          656 g         1317 t
ORIGIN      
        1 acctccacat ttactgcata aaatttctgt cctaaccatt ccaaaaccct ggtcggtttc
       61 gtaatcgatt gttccttttt caatctccct atcaaaagcc ggccatccgc agccactgtc
      121 aaatttagat gtagaagtaa ataattgctg accgcaacct gcacacacat aaataccatc
      181 ctcaaaaaaa tggtcgtatt cgccggtaaa agggcgttca gtgccttttt tcctcaaaac
      241 acgatattgc tcctcagaca atagtgactt ccattcatcc tctgatttaa ttacggtgta
      301 ttggcgcttt tctttcatgt tcgatttaac gtaaggagtt atttaaaagt tcaattattt
      361 attgaacaat atccgggttt gcttgttcac caaaattatg cacaacaaaa aaggatatag
      421 taaaatagta ttaatgataa tactttttac aggcgttatg tcaatgttat gtttaattac
      481 attattggta caccatgagg tagaaatatt taattaggat ttactttcct cgtatttaat
      541 tctatccttg atgatgccgg gcacttgctc gatgttaatt tttttgccaa atatgatttt
      601 atctttatct aacaaataaa tgagcggtgt ttgttccaca ttgtatttaa tttgatactg
      661 atctattccc tttgaattac tcacattaat ccaagggtat ttatgaccgc gtaaccaggt
      721 tttccattta tcaaattcat cttctgaata tactgcataa acttcagcgc cgagttgttt
      781 taattcagga tataatgcct cgattttcgg catttctttt ttacagtgcc cacagtcggg
      841 actccagaaa acaattactg taaatcgtga atttatactg tataatggta tatccacccc
      901 agatgtatct tgtaaaatta agggttgtcc tttttttcct aacagtgtag ggcgaacctt
      961 atcagcctgc gcctgaatac gatataagcc tacatcatct aaccaaaacg ctttgccacc
     1021 ggtataaaat ttatctacga tatgcacata aacagcatcg aagcccatta tttttgaggt
     1081 ggcatattca tttaataata aaacagttac atattgaaat gtttttggtg ataccgttga
     1141 tttgccaata ataaaatcta cggcaaccat gatagaatca ggcattttta ctacataatc
     1201 tttgatgtat ttttttatcc tggtatcata taaattactt cttaataaac gttcatcact
     1261 aaaatcgaaa ttgtcgaaat agtgctggcg aacataacgt aatgcaaatg ttgaatcttt
     1321 tgtgccatca ggattaaccg gggcttccgg aattttaata tctgtagcag cttttaagaa
     1381 tttagtatat aacagattgg gatatttatt aaaattagcc tcacggtatg cctccacctc
     1441 tgcattaata gccttcattt cagctttaat ttttacaccc gcttctgtag tttctccggc
     1501 caaatccaat tcttttttaa gctgagtcat tttatccttt tttgatgcga taaaatttaa
     1561 atcttcataa aaaatagtgt tttctaaaga gccgcttaca cgcatgtttt taataaaatc
     1621 aacagtatcg gtttccagac taaattttgg ttccatacca cttaccagca tttcgaaata
     1681 tttgtttttc aaagtaggaa taaccaccag ataaacacca cccggtaagg tatcattgct
     1741 tgtaaaaacg gcttcacctt tactgtttac cggaactgta tcgataacat attgagatga
     1801 accataatga tgacctaaat acaatgcagt atctgccaat cctttaattt gaattttaaa
     1861 ttcatatcca tgtaaagttt gaccgaatag gttggagagt aaaaaactac aaataaggag
     1921 gaaaactact cttttcattg aattagctat ttagtttggt taaagccgtg tatgccggta
     1981 aagcctcagc taccacaaaa atactgccgc atattaaaat catatcattt ttcccggcat
     2041 tttgtaaggc ttgttgtaaa gctgcaaata cggaagtgta ggaagtccct tttaaaccct
     2101 ttgacaatgc aagttcctgt aaatgaactg catcgaggcc acgaggaatg tctggtttac
     2161 aaaagtaata aatggcatga gcagggaata ggctgagccc ggcatccagg tctttatcat
     2221 taacagcccc ggttaccaca tgcaattgtt gataggattg ttcctttacc tgcctgaata
     2281 ataaggtcaa accacctgta ttgtgcgcac aatctgcaac taataatgga tttttctgca
     2341 atacctgcca tctgccggca aaaccggtat gggtttgcac atcccgcaac ccttcatata
     2401 ttgcttcgtc aggtatttct attccctgta atcgcattac ctcaatggtt tgaatggctg
     2461 tggcaacatt ttgtaattgg taatccccgg ttaagtccaa aatcaaatta ggatatgtga
     2521 gttggttcaa ataactcaca tttaattcca tgctatcaaa actgtggtct aacaattcca
     2581 cctgaacatt ttccggagcg ggatataaag ctgcattatt ttcagtagcc ttttgttcaa
     2641 aaacaccaat agtttcatcc tgtatttcac caataactac cggaatctgt ggtttaataa
     2701 tgccggcttt ttcagctgca attaatggaa gcgtattacc cagcatttgc atatggtcga
     2761 aactgatatt cgttataact gacaccaatg gctgtattac atttgtagaa tctaatctac
     2821 cgcctaatcc aacttctaca atagcaattt gcacattttt atacctgaaa taatcaaatg
     2881 ccattgctac ggtaatttca aaaaatgatg gctgaatgat gtcaatatgc ggttttattt
     2941 tttctacaaa tgttattaca aaatgttctt cgcacaattg cccgttaacg cgaatacgct
     3001 cgcgaaagtc aattaaatga ggtgatgtat ataatccagt gttataacct gcttctgcac
     3061 aaacagcagc caacatattg ctcacagagc cttttccatt ggttccacca acatgtatag
     3121 aagtaaatgt atgctgaggg ttatccaaaa cctgcaataa tgccaaagta ttcgtcaggt
     3181 cttttttata agcagcagca ccaatacgct gatacatcgg caagtgcgta aacaaataat
     3241 caagtgtttc cttatatgtc atcataataa catagcaaat agacacaata atcggctaaa
     3301 ataaaaaggg gagatgtgta taaaaaaaca gtgccttcat tttactgaag gcactgctct
     3361 tttcatataa tacttccggt cgtggcggga aatattattg taatttaaag tggaaagtaa
     3421 tatatcctat ctgtgtttca ggtgcttttg aatctgcgtt aaatgtagat ttcaaagcag
     3481 catttttcgc ctgtgtaatc aaatatgagt cggtaatagt tgaaccactt cgttcatatt
     3541 cggctctggt aactttcccg tttttatcta cataaatttt aattcgcacg tcaccaatct
     3601 cgttgtgttc aacttgtaat gaaggttttg ccttcatact acgaccagct aaatcccaac
     3661 caacattgcc atcatcacct tttccggcac ctggaccatg atatccgttt gggtcgccat
     3721 tggtagaacc ctggttgcca ccataggtag aattaccttc gctattaccg gattgaccat
     3781 tgttattcac cttatcgccg gtaaaaatct ggctttggtc aatagtgggt ttagtttcag
     3841 tttgagtatt agtattggtt ttaggcttat cattcttttt aacatccgga attttaaccg
     3901 attgctcaat gtcctgagta gcaatttcct cctttactgt ctgatt
//
LOCUS       DAORKW010000339         3939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1166_length_3939_cov_4.397528, whole genome shotgun sequence.
ACCESSION   DAORKW010000339 DAORKW010000000
VERSION     DAORKW010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1166_length_3939_cov_4.397528"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..279)
                     /locus_tag="PLB46_12070"
     CDS             complement(97..279)
                     /locus_tag="PLB46_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77303.1"
                     /translation="MQQKFNQLKALVALAEEDMIKYSLKGNKTAGTRMRKSLQDIKEI
                     STELRKEVLESRKMKV"
     gene            complement(408..1649)
                     /locus_tag="PLB46_12075"
     CDS             complement(408..1649)
                     /locus_tag="PLB46_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HRF77304.1"
                     /translation="MDIFDKLRKDRGPLGKWSDVAHGYMMFPKLEGEIGPKMTFRGKE
                     RITWSLNNYLGLANHPEVRKIDAEASAQFGLAYPMGSRMMSGNSDYHEQLERELSAFE
                     MKEDTFLLNYGYQGIMSVIDAICSRHDVIVYDAESHACIIDGVRLHQGKRFVFRHNDI
                     ENCEVQLKRATEMVQGTPGGILVITEGVFGMGGDQGKLKEIVDLKSKYNFRLLVDDAH
                     GFGTMGETGAGTGEAQGCQDGIDLYFSTFAKSMASIGAFISGEKDVIWYLRYNLRSQI
                     YAKSLPMPIVIGNLKRLELLRSKPELKAKLWDNVNKLQDGFRSRGFDLGNTNSPVTPV
                     FLQVSIPETINLVVDLRENHGIFCSAVTYPVIPKGMLMLRIIPTSSHTDDDIQKTLDA
                     FSAIREKINSGAYLKEDLVKI"
     gene            1784..2638
                     /gene="bamD"
                     /locus_tag="PLB46_12080"
     CDS             1784..2638
                     /gene="bamD"
                     /locus_tag="PLB46_12080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRF77305.1"
                     /translation="MFINRVIAVAIILISFGVFTGCQYDKLMKSDDFDAKLVKAREYY
                     AEGDYAKALPLLDQLLTVKIGTPEEKEIRYYMAYCYYGQGDYFSSSSLFKQVFNIFPL
                     SGEAEESLFMSAKSMYDASPRYQLDQTYSYKAIESFQYFIDVYPKSTLVTDANKYMDN
                     LRAKLEAKMIASAELYYNTEHYQAAAVTFKNVLLDFPDTRDAEEISFKIVNSWFSYAG
                     QSVVCRRPERYDMAIESYFDFIKQYPQSSKADQAKSLYERSIALKDKANNEITTYKIN
                     CDELTKEN"
     gene            2616..2966
                     /locus_tag="PLB46_12085"
     CDS             2616..2966
                     /locus_tag="PLB46_12085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRF77306.1"
                     /translation="MSSLKKINTQLSPSIEVQSLKTIENNSGNIYKSIFMVSKRAEQI
                     TQVVRGELHSKLEEFATHQDNLEEVFENREQIEISRYYEKLPKSTLIALEEFKNGQLY
                     WRDRDGGEETKETK"
     gene            2966..>3939
                     /gene="coaBC"
                     /locus_tag="PLB46_12090"
     CDS             2966..>3939
                     /gene="coaBC"
                     /locus_tag="PLB46_12090"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRF77307.1"
                     /translation="MSLQGKKILLGVSGGIAAYKSALLTRLLIKQGAEVKVMMTPGAK
                     AFIGALTFSTLSKHPVAVDFYNESDGTWNNHVSAGMWGDIILIAPATANTIAKMSVGI
                     SDNFLITTYLSAKCPVAIAPAMDLDMWLHKATQGNINTLKNNGVMVIAPESGELASGL
                     IGEGRMAEPEHIVSWLEDFFSTQPKRLSGKKVLITAGPTHEAIDPVRIIGIHSSGKMG
                     FALADAFAAEGAIVTVVHGPVSSSPQNAAVKTIGVTSAEEMLNSCQQIFPETDIFIAA
                     AAVADYTPANPSDKKIKKGDNPDETLNISLKRNPDILKTLSSQRNKQF"
BASE COUNT         1213 a          820 c          781 g         1125 t
ORIGIN      
        1 gcataagcca cattggtacc cagtgaaaaa aagtccgccg aggcggacca aaataatctg
       61 aatgcgaaat acatcgcaat gaggagggac taaaaattac accttcattt ttctagactc
      121 cagtacttct ttacggagtt cggtggaaat ttcttttatg tcctgcagac ttttacgcat
      181 gcgggttcct gccgttttat taccttttaa ggaatactta atcatgtctt cttcggcaag
      241 agcaacaagg gctttgagtt ggttaaattt ttgttgcatg ttcataaaat aaagtgtgtt
      301 aaaaaaaaca gttacaatgt ttggcagggt tcaattgcca aaaatgtttt aaggagacgc
      361 aatatagtaa aaatgccgga aattaccttt ccggcatttt aaaaaaatta tattttaact
      421 aagtcttctt tcagataagc gccgctgtta attttctcgc ggatagcaga gaaagcgtcc
      481 aatgtttttt ggatatcatc atctgtatga gaagaggtag gaataatacg caacatcaac
      541 atccctttag ggataaccgg ataggtaact gctgagcaga agataccgtg gttttcgcgc
      601 aaatccacaa ccaggttgat tgtttcagga atactcacct gtaaaaatac aggggttaca
      661 gggctattgg tattacctaa gtcgaaaccg cggctgcgga agccatcttg taatttattt
      721 acattatccc acaacttagc ttttaattca ggtttgctgc gcaataattc aaggcgtttg
      781 aggttaccaa ttacaattgg cattggtaag cttttcgcat atatctgcga gcgaagattg
      841 tatcttaaat accagataac atctttttca cccgaaatga atgcaccaat acttgccatt
      901 gattttgcaa aagtggagaa gtataaatcg ataccatcct gacaaccttg tgcttcaccg
      961 gtacctgcac cggtttcacc catagttcca aaaccatgtg catcgtccac taataagcgg
     1021 aaattatatt ttgatttcag gtcaacaatt tctttcagtt taccctgatc gccacccatg
     1081 ccaaatacac cttcagtgat aactaatata ccacccggag tgccttgaac catttcggta
     1141 gcacgtttta attgcacttc gcaattttcg atatcattgt ggcgaaacac aaaacgtttg
     1201 ccctggtgta aacgaacacc atcaataata catgcatggc tctcagcatc gtaaacaata
     1261 acgtcatggc gactgcaaat tgcgtcgata acgctcatga taccctgata accataattt
     1321 aataagaatg tatcttcttt catttcaaaa gcactcaact cgcgttcaag ttgctcatga
     1381 taatctgaat taccactcat catacggctt cccattggat aggcaagacc aaactgtgct
     1441 gaagcttcag catcaatttt tctcacttcc ggatggttgg ccaacccgag gtaattgttt
     1501 aaactccagg taattctttc tttacctcta aaggtcattt tcgggccaat ttcgccttcc
     1561 agttttggaa acatcatata accatgtgcc acatcactcc attttccaag cggacctctg
     1621 tcttttctta atttgtcaaa aatatccata gctgctttag gtttactttt tttaagggtg
     1681 caaagatatt accttaactt tggtcttaaa aatttaaggc aaagttttca ataaaaatca
     1741 attatctgcg tttaaggtta aatttattaa ttttgcgcct cttatgttta ttaatcgggt
     1801 tattgctgtt gcaattatac taatttcttt tggagttttc accggctgtc aatacgataa
     1861 gttgatgaaa agtgatgatt ttgatgcaaa actggttaaa gcccgtgagt attatgcaga
     1921 gggcgactac gccaaagcct taccattgct tgatcagttg cttacagtaa agattggaac
     1981 acccgaagaa aaggaaatca gatactatat ggcttactgc tattacgggc agggcgacta
     2041 cttttcatca tccagcctgt ttaagcaggt attcaatatt ttcccattgt cgggcgaagc
     2101 cgaagagtcc ttgtttatga gtgcgaaatc gatgtacgat gcctctccgc gttaccagct
     2161 tgaccaaact tacagctata aggctataga atcgttccag tattttatcg atgtatatcc
     2221 taaaagcact ttggtgaccg atgctaacaa gtatatggac aacctgagag ccaagctgga
     2281 agcgaaaatg attgcctcag ctgaacttta ttacaatact gagcattatc aagcagcagc
     2341 agttaccttt aaaaacgtat tactcgattt ccctgataca cgtgatgcgg aggaaatcag
     2401 tttcaaaata gtaaacagct ggttcagtta tgcaggccaa agtgtggttt gccgccgacc
     2461 agaacgctat gacatggcca ttgagagtta ttttgacttt atcaaacaat accctcaaag
     2521 ttccaaagcc gatcaggcta aatcattgta cgaaagaagt atagccttaa aagacaaagc
     2581 caataacgaa ataacaacat ataaaatcaa ttgcgatgag ctcactaaag aaaattaata
     2641 cacagttatc accttcaatt gaagtgcaga gcctgaaaac gatagaaaat aattcaggaa
     2701 acatctacaa gtccattttc atggtaagca aacgtgctga acaaattact caggtagttc
     2761 gtggtgaatt acacagtaaa ttggaagaat ttgcaaccca tcaggacaat ttagaagaag
     2821 tatttgaaaa ccgtgagcag attgagattt cacgttatta cgaaaaactt cctaaaagca
     2881 ccctgattgc gctcgaagaa ttcaaaaacg gccaattata ctggcgcgac cgcgatggtg
     2941 gtgaggagac taaagaaact aaataatgtc cttacagggc aaaaagatcc tgcttggtgt
     3001 aagtggcggt atagctgctt ataaaagtgc cttacttacc agattactca taaaacaagg
     3061 cgctgaagtt aaagttatga tgaccccggg ggcgaaagcc tttatcgggg cattaacttt
     3121 ttctacccta tccaaacacc cggttgctgt agatttttat aacgaatcgg atggtacctg
     3181 gaacaaccat gttagcgcag gcatgtgggg agatattatc ctgatagcgc cggcaactgc
     3241 aaatactatc gctaaaatga gtgtaggtat cagcgataac ttcctgataa ccacctactt
     3301 atccgctaaa tgcccagtgg ctattgcacc tgctatggac ttggatatgt ggttacataa
     3361 ggctacacaa ggtaatatca acacgttaaa aaataatggt gttatggtca ttgctcctga
     3421 gtcgggagaa cttgccagcg gccttattgg cgaaggcaga atggctgaac cggagcatat
     3481 tgtttcctgg ttggaagatt ttttttctac ccaacccaaa cgccttagtg gcaaaaaagt
     3541 attaataacg gcaggtccga cgcatgaagc tattgaccct gttcgtatta taggcatcca
     3601 ttcctccggt aaaatggggt tcgctcttgc cgatgctttt gcggctgaag gtgcgatagt
     3661 taccgttgtg catggccccg ttagcagcag tccgcaaaat gctgccgtaa aaaccattgg
     3721 cgtaaccagc gctgaggaaa tgctgaacag ttgccagcaa attttccccg aaaccgacat
     3781 ctttattgct gccgctgcgg tggcggacta tactcctgcc aatccttcgg acaaaaaaat
     3841 aaaaaaaggt gataaccctg atgaaacatt aaatatcagc ctgaaacgta atcccgatat
     3901 attaaaaacc ctgagttcgc agcgaaataa acagtttat
//
LOCUS       DAORKW010000340         3922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1178_length_3922_cov_4.848203, whole genome shotgun sequence.
ACCESSION   DAORKW010000340 DAORKW010000000
VERSION     DAORKW010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1178_length_3922_cov_4.848203"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1026
                     /locus_tag="PLB46_12095"
     CDS             208..1026
                     /locus_tag="PLB46_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77308.1"
                     /translation="MKHTLTAAMLMVCTLCNLSAQTGYSDAGARSNSMGNASVTFQDV
                     FSTQNNQAAMAYVETPSAGISTQNYFLVEGGISSHYGVFAMPVQKAGVFGLSANYTGD
                     NTFNQTKIGLGYGRKLAENVAVGLQLDYVGTKTSEVGSGAAFTFDIGVLYQPNKVLSI
                     GAKVFNPVRAKNNLDFEEELPSIINVGFAYQPSEKIVVCIEGEQNLQDDLRIKSGVEY
                     HIIEQLYLRGGYLSNPSMFTTGIGLKIKTLQLDLSSQFHQQLGVTPGLGIRYDF"
     gene            1040..3148
                     /locus_tag="PLB46_12100"
     CDS             1040..3148
                     /locus_tag="PLB46_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77309.1"
                     /translation="MHNFPSPKKHFIQHLVFITLMIVCSNTIFAQENKAEGKLLEDDM
                     IEGAAEKVDNSDISIDSYQENLTYLLQNPININKAGYLDLRNSGLFTEVQIRDLLEHI
                     KSFGPLLNIYELQTIASFSNEDIRIIKPFIRLDGSSVNAVPFHKKLTDGDYQYFFRAS
                     RYVEVQDGYIADSTGSTTYAGNNLRIYSRFRYNYQNNLSYGITMEKDAGEAVFGPTQP
                     YGFDYYSMHFFKKGTGNLRALALGDYEVRIGQGLTMWSGFGIGKSVYPVAIRRAGPVL
                     DSYSSTNENRFMRGAAVTMGLNNWQVTAFASHKALDANIDLIDTVSDEILQVSSIDES
                     GLHRTDNELERKNAINETIGGFDATYYKGQFNAGIAAVYYNLSSALSPPTEPYQLYDF
                     AGNTLLNASIHYNYLWRNLLLFGETAVSDNLKVATVNGIILPVDPKVDIAVLHRYFSP
                     EYQSLYAATFSEGTLPQNEQGTYFGIEVKPIKGWKVSGYLDVYKHPWLEFRTDAPTYG
                     TDFLGQIFWQPSRAFETYIRYKHEISDRNLSNDYLEEEPNLHVITSLAKSNIRWHADY
                     DVNKSLTLKSRIEFTLYEEAEGVPERGYLLFQDFNYHPLSSKFSFATRFAIFNTDTYN
                     SRIYAYETEVLYAYSIVGLSGKGTRTYLLVTYSPTKWLDIWARAATTWYSGEEEVGSG
                     DNTFPGLTRSDVKLQFRFKW"
     gene            complement(3149..3688)
                     /locus_tag="PLB46_12105"
     CDS             complement(3149..3688)
                     /locus_tag="PLB46_12105"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRF77310.1"
                     /translation="MTHNYQTAPMAKRVTAFIIDMIIVGMVTSAFLFVSMGKTPEKLQ
                     ASGDEGKPSLMDKYQFFAYFEPIALDPNRGIYLENFLKKYKMQSVVGLFLLPLLYFVL
                     LEGMKGATIGKMMTGIRVRRKDGGKISFGTAFLRHIGRLISATIFMLGFLLAFIDGKR
                     QALHDKIANTMVLKKGTGI"
BASE COUNT         1286 a          691 c          720 g         1225 t
ORIGIN      
        1 acccctgcac tttttacata ggtaaatatc aatgccaata ctcccaacaa agggatggca
       61 taaagaatca tgtttacatc cataatttaa cgatttgttt aagttagtct ttgaggtcgc
      121 taaaataggg ctttgacctc atattattca acaattgggt gtgaacagtc gagaatttga
      181 aatataattt catttatctt taccctaatg aaacatacac taacagccgc aatgttaatg
      241 gtttgcaccc tatgtaatct cagcgcacaa acaggctata gcgatgccgg tgccagatcg
      301 aacagtatgg ggaatgccag tgttacattt caagatgtat ttagcactca aaacaatcag
      361 gctgcaatgg cttatgtgga aactccatcg gccggaatca gcacacaaaa ttatttttta
      421 gtagaaggtg gtattagtag tcattacggt gtttttgcta tgcctgtgca aaaagcaggc
      481 gtgtttggac taagtgcaaa ttatacaggt gataatactt tcaatcaaac aaaaataggg
      541 ttaggatatg gtcgtaaact tgcagaaaat gttgctgtgg gattacaact cgattatgtt
      601 ggaactaaaa cgagcgaggt aggttcaggt gcagcattta catttgatat cggtgtgtta
      661 taccaaccta ataaagtgct ttcaattggt gcaaaagttt ttaatccggt aagagcaaaa
      721 aacaatttag attttgaaga agaattacct tcaattatta atgtcggatt tgcctatcag
      781 ccctccgaaa aaattgttgt ctgtattgaa ggcgaacaaa atttacagga tgatttaaga
      841 attaaatccg gagtagaata tcatattatc gagcagttat atttacgcgg aggatattta
      901 tccaatcctt ccatgtttac aacaggcatt ggattaaaaa taaaaacctt acaactagat
      961 ttaagtagcc agtttcatca gcagttaggt gtaacaccag ggctaggaat tcgttatgat
     1021 ttttaaataa ttatccctca tgcacaactt tccttcacca aaaaaacatt tcattcaaca
     1081 tttggttttt ataaccttaa tgattgtttg cagcaatact attttcgcac aggaaaataa
     1141 agctgaagga aaattattgg aagacgacat gattgaaggt gctgcggaaa aagtagacaa
     1201 ctcagatatt tcgattgact cttatcagga aaatctcacc tatttattac aaaatccaat
     1261 aaacattaat aaagcaggat atttagattt acgaaattca ggtttattta ctgaagtaca
     1321 aatccgcgac ttattagaac acattaaatc cttcgggccc ttattaaata tttacgaatt
     1381 gcaaacaatt gcatcatttt caaatgagga tattcggatt ataaaaccat ttattcggct
     1441 tgatggcagt tcagtaaatg cagtaccatt tcacaaaaaa ttaacggatg gtgattatca
     1501 atattttttt cgtgcaagtc gttatgtaga agtgcaagat ggttatattg ctgatagcac
     1561 cggttctacc acatatgcag gaaataatct gagaatttat tcgcgtttca gatataatta
     1621 tcaaaataat ctgagttatg gcataaccat ggaaaaagat gcaggcgaag ctgtattcgg
     1681 tcccacccaa ccttatggat ttgattatta ttccatgcac tttttcaaaa aaggaaccgg
     1741 taatttacgt gcattagctt tgggtgatta tgaagttcgt attggtcagg gacttaccat
     1801 gtggagtgga tttggcatcg gtaaaagtgt ttaccctgtt gctattcgaa gagcaggtcc
     1861 tgttttagac agttactctt ctaccaatga aaacagattt atgcgcggtg cagctgtaac
     1921 tatgggatta aataattggc aagtaacggc atttgcttct cataaagcac tcgatgctaa
     1981 tatcgattta attgataccg tttcagatga aatattacaa gttagcagca ttgatgaaag
     2041 tggtttgcac agaacagata atgaactgga aagaaaaaat gctattaatg aaacaattgg
     2101 tggttttgac gccacttatt ataaaggaca atttaatgca ggtattgctg cggtttatta
     2161 caatttaagt tctgcattat ctccacccac cgagccttac cagttatatg atttcgcagg
     2221 aaacacctta ttaaatgcaa gcattcatta taattattta tggcgtaatt tattattgtt
     2281 tggtgaaaca gcagtcagtg ataatttaaa agttgcaact gtcaacggca taattttacc
     2341 cgttgaccca aaagttgata ttgctgtttt acatcgttat ttttcacctg aatatcaatc
     2401 actttatgct gctacttttt cggaaggtac tttacctcaa aatgaacaag gcacttattt
     2461 tggcattgaa gtaaaaccaa ttaaaggttg gaaagtaagt ggttatttag atgtatataa
     2521 acatccgtgg ttggaattta gaactgatgc tccaacttac ggcactgatt ttttaggtca
     2581 aattttttgg caaccatcgc gtgcatttga aacttacatc cgatataaac atgaaatcag
     2641 cgaccgtaat ttatcgaacg attatttaga agaagaaccc aatttgcatg tgattacctc
     2701 attggcaaaa tctaatattc gctggcatgc cgattatgat gtaaataaat cgctgacgtt
     2761 aaaatcaaga atagaattta cattatacga agaagccgaa ggtgttcctg aaagagggta
     2821 tttattattt caggatttta attaccatcc actcagcagc aaatttagtt ttgcaacacg
     2881 cttcgccata tttaatactg atacttataa ctccagaatt tatgcttacg aaaccgaagt
     2941 gctttacgcc tattccattg tcggtttaag tggcaaagga acaagaacct atttattggt
     3001 tacttattca cctaccaaat ggctcgacat ttgggcacgc gctgctacta cctggtattc
     3061 aggtgaagag gaagttggtt cgggtgataa caccttcccc ggtttaacac gtagtgatgt
     3121 gaaattacaa ttccgcttta aatggtaatt aaataccggt gcctttttta agcaccatag
     3181 tattggcaat tttatcgtgc agggcctgac gttttccgtc tataaaagcc aataaaaatc
     3241 ccaacataaa aatagttgcg gaaatcaacc tgccgatatg acgtaaaaaa gcagttccaa
     3301 aggaaatttt tcctccatct ttccgacgca ctcttatacc tgtcatcatt ttaccaatag
     3361 ttgcaccttt cattccttct aaaagcacaa aatataataa tggcaataaa aacaaaccta
     3421 ctacagattg cattttatat tttttaagga agttttccag gtaaatgccg cggttcgggt
     3481 ctaaagcaat aggctcaaag taggcaaaaa actgatattt atccatcaaa cttggtttac
     3541 cttcatcacc agatgcctgc aatttttcag gagtttttcc catgctaaca aataaaaatg
     3601 ctgaagtaac catgcctaca ataatcatgt cgattataaa tgcagttacg cgtttggcca
     3661 taggtgctgt ctggtaattg tgtgtcataa atttagtttt aaaccaataa atttcttttc
     3721 aattgcaaag ataacaacaa tttgaaaagc aaacatttac tgcatgttga aaccgggata
     3781 aacccatctt tggcagatga gtagatatac ctgtctgaaa atagcgcaat accagcatat
     3841 tcagcaggtt atgcctgaaa taggactggt tgattgcagc aacctataca aagtttcccc
     3901 tgtcaaagga tgttaatcgg ca
//
LOCUS       DAORKW010000341         3894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1191_length_3894_cov_4.978640, whole genome shotgun sequence.
ACCESSION   DAORKW010000341 DAORKW010000000
VERSION     DAORKW010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1191_length_3894_cov_4.978640"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..583
                     /locus_tag="PLB46_12110"
     CDS             80..583
                     /locus_tag="PLB46_12110"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRF77311.1"
                     /translation="MKHLKMLVFTLFVTTAVFAQAQGKIAYINSQDLLQVLPEAKDAN
                     AKLTAYAKEFEVQFQSYYAEYQALAAELTNNTTLSEVAREAKLQDAANLEQRLAQYEQ
                     ESNTKIEAKKAELFQPIITSTTELIKTVAKENGYTYVIDNSLAVLIMAPEGDDILPLV
                     KKKLNIQ"
     gene            complement(666..3119)
                     /locus_tag="PLB46_12115"
     CDS             complement(666..3119)
                     /locus_tag="PLB46_12115"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRF77312.1"
                     /translation="MRKFLPLLYFLTFILTANAQDIQRKQLVASRISGNIHIDGNLNE
                     PDWQDVAEATDFTQFQFVYGKPSAYATSIKVMYDNTAIYIGATMYDPQPDSIQRDLAA
                     RDEFGSADFIGIIMDPYQEGVNGIEFFVTASGVQAEAKTYPNSNNGEDFSWDAVWVSA
                     AKITEKGWVAELKIPFSAIRFPNKDEQSWYINFVREVKRNRDKSAWNAIDPQVQGFVN
                     QSGILTGINNIKAPVRLSLSPYFSTYYDVFNDKANDVSAESWSFNGGADLKYGLNDAF
                     TLDMTLIPDFGQVQSDNQILNLTPFETYFVEQRQFFTEGTELFNKAGLFYSRRVGATP
                     IGYYDVLYGTDPSSEIISNPSQARLLNAAKLSGRTTGGLGIGVFNAIEGETIAVVKDS
                     AGVETSTVTGPRANYNVLVFDQSLKNNSYIAFTNTNVLRENAFNDANVTAVNFSFNDK
                     PNIWAIEGGGAYSQKYTGVETAANNGIYHYLSAGKSGGKRRFAVGYEYTGATYDNNDL
                     GFLPINNLLNFFANTSYHTYEPLWHLNQLNLTVDVNYTRIINPDAFHNFSINLDAWGN
                     FRNFIFMGLWIYTEPVITYDYFEPRSPGRYYTYPINYNFGGWISSDYSKRFAFDASSN
                     LRLFGEEGRYRHNIDFSPRFRVNDKILLTAYGGRFFWPNDVGWVNYYGDSIILGNRDN
                     VTFEGLVQANYTPNVKMSFGLRLRHYWSFAEYSAFYNLGDDGSLLPTTYNSFDADGNS
                     TDDVNFNAFNVDFVYTWFFAPGSELNIVWKNSIYQFGTYLPENYLDDMDQVFDAPVGN
                     NFSIKLLYYLDYLYLKKKQ"
     gene            complement(3254..>3894)
                     /locus_tag="PLB46_12120"
     CDS             complement(3254..>3894)
                     /locus_tag="PLB46_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF77313.1"
                     /translation="NALNCIYDAKSAVRYVREHAEDLNIDPNKIVVGGGSAGGFLTLQ
                     LAINDSKWQDPKDNTATSCMPNAMVLLNPVVNSMEHEFRIVKFKDNEADSNSASHALE
                     VNPLTNIKSGMPPAVVFHGTADKISAYQFVEQYCADYEKAGNTITLNTYEKMKHGFTA
                     PKFQNGKYYRETLRKTDEFLISLGYLTGSPNVPEPDPKENPKPKAQKKTKPE"
BASE COUNT         1259 a          737 c          652 g         1246 t
ORIGIN      
        1 gggcaaggct gaccggtgat ttggaggtac cacccattta cttatctttg cgcttcaact
       61 ttaaaataga atagtatata tgaaacactt aaaaatgttg gtatttacct tgtttgttac
      121 tactgcagtt tttgcacagg ctcagggtaa aattgcctat atcaactctc aggacttatt
      181 acaagtttta cctgaagcaa aagatgccaa tgctaaatta acagcttatg ccaaagaatt
      241 tgaggtacaa tttcaatctt attacgctga atatcaggct ttggctgctg aattaaccaa
      301 taatactaca cttagcgaag tagctcgtga agcaaaatta caagatgcag caaatttaga
      361 acaacgtctt gcacaatatg agcaagaatc taacacaaaa attgaagcta aaaaggcaga
      421 attatttcaa cctattatta cttcaacaac cgaattaatt aaaactgtag caaaagaaaa
      481 cggttacact tatgtaattg acaattcttt agcagtatta attatggcac cggaaggtga
      541 tgatatttta cctttagtaa agaaaaaatt gaatattcaa taatcgcatt tattttataa
      601 aaaaagtcgg gtaacaattg ttatccgact tttttatttt atttacatct gtaaagttga
      661 cgctgttatt gttttttctt taaatataaa tagtcgagat aatacaataa tttgatagag
      721 aagttattgc caacaggagc atcaaacacc tgatccatat catctaaata attttcaggt
      781 aaatacgtgc cgaactgata gatggaattt ttccaaacaa tatttaattc actaccagga
      841 gcaaaaaacc aggtgtatac aaagtccaca ttaaatgcat tgaaattaac atcatcagtt
      901 gaattaccat cagcgtcaaa tgaattgtaa gtggtaggta ataaactacc gtcgtcacca
      961 agattataaa atgccgaata ctcagcgaaa ctccagtagt ggcgtaacct gagaccaaaa
     1021 ctcattttta catttggtgt gtaattcgcc tgaactaatc cttcaaatgt aacattatcg
     1081 cgattaccta aaataatgga atcaccataa taatttaccc aaccaacatc atttggccaa
     1141 aaaaatcttc caccataagc agtcaataaa attttatcgt ttaccctgaa acgtgggcta
     1201 aaatcgatat tatgtcgata gcgtccttcc tcaccgaata atcgcaaatt actgctcgca
     1261 tcaaatgcaa atcgtttact ataatctgaa gaaatccaac ctccgaaatt atagttaatc
     1321 ggatacgtgt aataacgtcc gggtgaacgc ggttcaaaat aatcgtaggt aattacaggt
     1381 tcagtatata tccagaggcc cataaaaatg aaattcctga aatttcccca tgcatctaaa
     1441 ttgatagaaa aattatgaaa agcatcagga ttaattatgc gtgtataatt tacatctacc
     1501 gttaaattta gttgatttaa atgccataaa ggttcatacg tatgatagct tgtattagca
     1561 aaaaaattga gtagattatt tataggaagg aaccctaaat cgttgttgtc gtatgttgca
     1621 ccggtgtatt cgtaaccaac tgcaaatctg cgtttaccgc ctgattttcc ggctgataaa
     1681 taatggtaaa ttccattgtt agcagccgtt tcaacaccgg tatatttttg actataagca
     1741 cctccacctt ctatggccca aatattgggt ttatcattaa acgaaaaatt aactgctgtt
     1801 acattggcat cgttgaatgc attttctcgt aatacgtttg tattcgtaaa tgcgatataa
     1861 gaattatttt tcaggctttg atcaaatacc aaaacattat aattagcgcg tggtccggtt
     1921 acagtacttg tttcaactcc ggcactatct ttaaccactg caattgtttc accctctatg
     1981 gcattaaata cgccaatgcc taaaccacca gtagtgcggc ctgataattt agcagcattt
     2041 aataatctgg cttgcgaagg attactgata atttcagaag aaggatctgt accgtataat
     2101 acatcataat atccaatcgg agttgctcct acccttctgg aataaaacaa tcctgctttg
     2161 ttaaataatt cagttccttc tgtaaaaaat tgacgctgtt ctacaaaata agtttcgaaa
     2221 ggtgttaagt ttaaaatctg attatcactt tgcacttgtc cgaaatcagg aatcaacgtc
     2281 atatccaaag taaaggcatc gtttaatccg tattttaaat cggctccgcc attaaaactc
     2341 caactttcgg cgcttacatc atttgctttg tcattaaata catcatagta agttgaaaaa
     2401 taaggtgata atgataatcg aaccggtgct ttaatattat taattccggt taatattccg
     2461 ctttgattaa cgaaaccctg cacctgaggg tcaatggcat tccatgcact tttatcacgg
     2521 ttgcgtttaa cttcacggac aaaattgata taccagcttt gttcgtcctt gttggggaat
     2581 cgaatggcag aaaacggaat ttttaattca gccacccaac ctttttccgt aatttttgct
     2641 gcactcaccc aaacagcatc ccagctaaaa tcttcaccat tgttgctatt gggatatgtt
     2701 ttagcttccg cttgcacacc acttgcggtg acaaaaaatt cgataccatt gacaccttcc
     2761 tgataagggt ccataattat tccgataaaa tcagctgaac caaattcatc acgagctgct
     2821 aaatctcttt gtatactatc aggttgtggg tcatacatgg ttgctccaat ataaatggcc
     2881 gtattatcgt acattacttt tattgaagtt gcatatgccg aaggtttacc atatacaaat
     2941 tggaattgtg taaaatctgt tgcttcggca acatcctgcc aatcaggttc attcaaattg
     3001 ccatcaatat gaatattgcc ggaaatgcgg gaagcaacca attgcttgcg ttgaatgtcc
     3061 tgcgcatttg ccgttagtat aaacgtcaaa aaatacaata agggtaaaaa ttttcgcatg
     3121 cctgaaaaat gtattacaat agtaaaaata ttacctcgat ttattacaaa ctgaggtgtt
     3181 aattgatgtt aaatgaaagc taaaaaaaaa gagcctgctg taacagactc tctttcttgc
     3241 ttgaaataat tacttattcc ggtttagtct ttttctgagc cttaggttta ggattttctt
     3301 ttgggtcagg ttccggcaca tttggtgaac cggtcaggta gcctaaactg attaaaaatt
     3361 catctgtttt acgtaaagtt tcacgataat atttaccatt ctgaaatttt ggagcagtaa
     3421 atccgtgttt cattttttcg taggtattta aagtaatggt attaccagcc ttttcgtagt
     3481 cggcacaata ttgttccaca aattggtaag cactaatttt atctgctgta ccatggaaaa
     3541 caactgcggg tggcattccg gatttaatat tagttaaagg atttacttcc aatgcatgtg
     3601 atgctgagtt agaatccgct tcattatctt taaatttcac aatcctgaac tcatgttcca
     3661 ttgagttaac tacagggttt aataatacca ttgcatttgg catacacgaa gttgctgtat
     3721 tatctttcgg atcttgccat ttactgtcgt tgatagctaa ttgcaaagtt aaaaacccac
     3781 cggctgaacc gccgcctact acaattttat ttgggtcgat attgaggtct tcagcatgtt
     3841 cacgcacata tcggacagca ctttttgcat cgtaaataca gtttaacgca ttgc
//
LOCUS       DAORKW010000342         3885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1199_length_3885_cov_4.943081, whole genome shotgun sequence.
ACCESSION   DAORKW010000342 DAORKW010000000
VERSION     DAORKW010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1199_length_3885_cov_4.943081"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLB46_12125"
     CDS             <1..413
                     /locus_tag="PLB46_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77314.1"
                     /translation="TLRWAWYCILIGLGLFLIFRSKRLQQPIPVLEPNENKSLEFIQT
                     IGRMYYLKKNHKQLAQQKLKLFLHFINERYRIPGQEIDEAYIEKVQLKSEISENHLKQ
                     IFKHIKYINNTNDVTDQDIVQLHELLDYFYKNCK"
     gene            421..1404
                     /locus_tag="PLB46_12130"
     CDS             421..1404
                     /locus_tag="PLB46_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRF77315.1"
                     /translation="MEENSFYQEMSPELQEVANSVQLIRNELKKVIIGQEKMIDLLIT
                     GLLCGGHILIEGVPGIAKTLTAKLLAKTIATDFSRIQFTPDLMPTDVLGTLVFNQKTN
                     DFSFKKGPIFSNIVLIDEINRSPAKTQAALLEVMEELKVSMDGESHAMGFPFFVIATQ
                     NPIEQEGTYKLPEAQLDRFIFRIKITYPDLKEEKQILYRFKEDFSNKQKDSVQAVVTV
                     QQLKKCRDVVEHIHIKDELLDYIANIVFKTRNHGDLFLGASPRASLNIMKASKAVAAM
                     NGRNFVTPDDIHFVTYPVLNHRIILTPDKELEGYDSASVIKSIIEGIDVPR"
     gene            1449..2735
                     /locus_tag="PLB46_12135"
     CDS             1449..2735
                     /locus_tag="PLB46_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRF77316.1"
                     /translation="MIGVIAALFAISFSVSILFYVAQACIFIAVILVLVDLFLLFNSR
                     SKLSATRKTSNALSLSDENNVHIIVRSKSPIPLMLHIIDELPYQLQKRDFAVDMKIAN
                     GQLLQIDYQVRPVIRGAYTFKNINIYAKSAIGLISRRYTFNEHLTVPVYPSLIQMKEY
                     ELKTLSRISFFEGVKKMRRIGHSYEFEQIKKYVRGDDVRSINWKATSRVGELMVNHYE
                     DERSQQVFCIIDKSRSMKMPFNGLSLLDYAINSTLTIANIALKKEDRVGLMSFSDRIG
                     TVLGPDKHHVQLRKILDALYNEKERFNEANYELLYLQIRNFIKVRSLIFLYTNFESLY
                     AIERVVAILRKLNKLHLLVVIFFENSEITDYAKSDAGNLEDVYLTTVAQKFAVEKKLI
                     RQELTKYGIQTIISTPEELSINTINKYLELKSRGLI"
     gene            complement(2732..3172)
                     /locus_tag="PLB46_12140"
     CDS             complement(2732..3172)
                     /locus_tag="PLB46_12140"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRF77317.1"
                     /translation="MEVLDYEKKPQQIAYAGFVKRLVANLIDFILVALAGWAIGLIFG
                     VNIFKESWGVKWFNNILTMIYFIVMEAGKNNATLGKQIVGIKVVTTNGAPITLQDSLV
                     RNLCKILSAALLMIGFIMVAFSAKKQGLHDMIAGTLVIDDKSKL"
     gene            3334..>3885
                     /locus_tag="PLB46_12145"
     CDS             3334..>3885
                     /locus_tag="PLB46_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HRF77318.1"
                     /translation="MKKSVLLMVFAGAAMLMFSCKETKIQNVKMENSADSLSYAIGVN
                     IGQSFKQQEITEVNTELMATVIKAILNGDTIGLKMNDSSSMAFLNTYMRKKQEMEMEK
                     DKEEGKKWLEENAKKSGVVVTPSGLQYSVIKSGNGISPVDGDTVTIHYVGKFIDGEEF
                     DNSIKSGQPVTYPVNDFIKGWTEG"
BASE COUNT         1325 a          644 c          698 g         1218 t
ORIGIN      
        1 aaacattgcg ttgggcttgg tattgcattt tgattggttt aggtttattt ttaatcttta
       61 ggtcaaaacg tttacaacaa cctattccgg ttttggaacc taatgaaaat aaatcgttgg
      121 agtttataca aacaatcggt cggatgtatt accttaaaaa aaatcacaag caacttgcac
      181 aacaaaaatt aaaattgttt ttacacttta tcaatgaacg ttacagaata ccggggcagg
      241 aaattgatga agcctatatt gagaaagtac aattaaaatc agaaatcagt gaaaatcacc
      301 taaagcaaat attcaaacac attaaatata tcaacaacac gaacgatgtt accgaccagg
      361 acattgtcca gttacatgag ttgttagatt atttttataa aaattgtaaa taagcttatt
      421 atggaagaaa attcttttta ccaggaaatg tcacccgaat tacaagaagt agctaattcg
      481 gtacaattaa ttcgcaatga acttaaaaag gtaattattg gtcaggaaaa aatgatagac
      541 ctgctcatta ccggattgtt atgtggcgga catattttga ttgaaggtgt tccgggtatt
      601 gccaaaacct taaccgcaaa gttgttagct aaaacaatag cgactgattt ttcaagaata
      661 cagttcacac ctgatttgat gcctactgat gttttgggaa cattggtatt taatcaaaaa
      721 acaaacgact ttagtttcaa aaaagggccg atattttcca atattgtttt aatcgatgag
      781 ataaaccgtt cacctgccaa aacgcaggct gctttgcttg aagtgatgga ggaactcaaa
      841 gtttctatgg atggagaatc acatgcaatg ggttttccgt tttttgtgat tgccacacaa
      901 aaccctattg aacaagaagg tacttataaa ttaccggaag cacagttaga ccgatttatc
      961 ttcagaatta aaattaccta tcctgattta aaagaagaaa aacaaatttt gtatcgtttt
     1021 aaagaggatt tcagcaataa acaaaaagac agtgtgcaag cagtggttac tgtgcaacaa
     1081 ttaaaaaaat gtcgtgatgt tgtagaacat attcacatta aagatgagtt actcgattat
     1141 attgcgaaca tcgtatttaa aacacgtaat catggtgatt tatttttagg cgcttcacca
     1201 cgtgcttctt taaatataat gaaggcatcc aaagcagttg ctgccatgaa tggacgcaat
     1261 tttgtaacac ctgacgatat ccattttgtt acctatcctg tgctcaatca ccgcataatt
     1321 ttaactccgg ataaagaatt agaaggctat gatagtgcat cagtaattaa atctataata
     1381 gaaggaatag atgttccgcg atgaaatcat cttttcgcaa tatatatatt aacaacaggt
     1441 tattctggat gatcggagta attgcagctt tgtttgcaat tagtttttcg gtttccattt
     1501 tattttatgt tgcacaggca tgtatattta ttgctgttat tttggtgctg gtagatttgt
     1561 ttctgttgtt taacagtcgc agcaaactat ctgctacacg caaaaccagt aatgcattgt
     1621 cgttaagcga tgaaaacaat gtgcatataa ttgttcggag caagtcccct attccactca
     1681 tgttgcacat tatcgacgaa cttccctatc aattacaaaa aagggatttt gcagtcgaca
     1741 tgaaaatcgc caacggccaa ttacttcaaa tagattatca ggtaaggccg gttataagag
     1801 gtgcatatac tttcaaaaac attaatatct atgcaaaatc tgcaattgga ttaatcagtc
     1861 gcagatatac gtttaacgaa catttaactg tccctgtata tccttccctg attcaaatga
     1921 aggagtacga attaaaaaca ttgtcgcgca ttagtttttt tgaaggggtt aaaaaaatgc
     1981 gccgtattgg acatagttat gaatttgagc aaattaaaaa atatgtacgt ggtgatgatg
     2041 tgagaagtat caactggaaa gcaaccagta gagttggtga gttgatggtt aatcattacg
     2101 aagatgaaag atcacaacag gtattttgta ttatcgataa aagccgcagt atgaaaatgc
     2161 cttttaatgg tttgagttta ctcgattatg ccattaactc tacattgact atagctaaca
     2221 ttgccttaaa aaaagaagac cgcgtaggac taatgtcctt ttctgatcgc attgggacag
     2281 tattaggccc tgataaacat catgtacagt tgcgtaaaat tttagatgcc ttgtataatg
     2341 aaaaggagcg ttttaatgaa gcgaattacg aattattata tcttcaaata agaaacttta
     2401 ttaaggttag aagtttaata tttttatata ccaatttcga aagtttatat gctattgaac
     2461 gggttgtcgc cattttgcgt aaactcaata aattacattt actggtagtt atatttttcg
     2521 aaaattcaga aatcaccgat tatgcaaaaa gtgatgccgg caatttggaa gatgtttacc
     2581 tcactaccgt tgctcaaaag tttgcagtag agaaaaaact catccgacaa gagttaacta
     2641 aatatggcat tcagaccata atttctacgc ctgaggaatt gtccatcaac accatcaata
     2701 aatatttaga gttaaaatcg cgtggattaa tttaaagttt tgatttatca tcaataacta
     2761 aggttccggc aatcatgtcg tgaagccctt gtttctttgc tgaaaacgcc accataataa
     2821 aacctatcat taataatgca gcagataata ttttgcataa atttctcaca agtgaatcct
     2881 gtaacgttat tggcgcgccg ttggtagtaa ctacttttat cccaactatt tgcttaccca
     2941 gtgtagcatt gttttttcct gcctccatta caataaaata aatcatagta aggatattat
     3001 taaaccattt cacaccccat gattctttaa aaatattaac cccgaaaatg agccctattg
     3061 cccaacctgc cagtgcaact aggatgaaat caatcaggtt ggctactaac cgttttacaa
     3121 aaccggcata tgctatttgt tgaggttttt tttcgtagtc taaaacttcc atatttcaag
     3181 gtttatttaa agttatcaaa tttcatccag acagcccttt tacagctcaa attatgccaa
     3241 aaatgggcaa aatggcttca aacgccttgt tttcaggctg tttcagtgca aatcaaacat
     3301 ttggcgtatc tttgcaccct caaaattcat aaaatgaaaa aatcagtgct tttaatggta
     3361 tttgcaggtg ctgcaatgct catgttctca tgtaaagaga ctaaaataca aaatgttaaa
     3421 atggagaatt ctgccgactc attaagttat gcaattggcg taaacattgg tcagagcttc
     3481 aaacaacagg aaattaccga agtaaatacc gaattaatgg ctactgttat aaaagccatc
     3541 ttaaacggtg atactattgg attaaagatg aatgattcca gttcaatggc atttctgaat
     3601 acttatatgc gtaaaaagca ggaaatggaa atggaaaaag ataaagaaga aggtaaaaaa
     3661 tggttagaag aaaatgctaa aaaatcgggc gttgtggtaa ctccttccgg cttacaatat
     3721 tctgttatta aatcaggtaa tggtatttca ccagtagatg gtgacactgt tactatccat
     3781 tatgtaggta aatttatcga tggtgaagaa tttgataatt ctatcaaaag cggtcagccg
     3841 gtgacttacc cggttaatga ttttatcaaa ggctggactg aaggt
//
LOCUS       DAORKW010000343         3872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1204_length_3872_cov_4.846476, whole genome shotgun sequence.
ACCESSION   DAORKW010000343 DAORKW010000000
VERSION     DAORKW010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1204_length_3872_cov_4.846476"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1876
                     /locus_tag="PLB46_12150"
     CDS             <1..1876
                     /locus_tag="PLB46_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77319.1"
                     /translation="DFARGTTGGNISTGGIYSLLTAAGANQVLYFQPTADDFTPGTIT
                     YVYKNITGTLLTELDFSYVIYLYNNENRATSLTLSYSTDDITYTPVPSVDFTSFAALD
                     VLLYTFNRSVSLTGLSIPNNTNIYFKWSTDDVPGGAGNRDEIGLDNITLTAGSDPIPT
                     PTVYFNNDIVYANENDGSYAFDINIATPHDCDAFMTLNAISTAKSGIDYSFTFPAMVS
                     FEAAGPTTHTITIPLIDDAIPEYMETMYIELDSVSPGCLITAPDFQLVFIEDSDVAVI
                     GDCENLIISQYGDGTGLLNNKALEIYNPTDASINLEDYYVHIYANGAISPIYYFQCAG
                     MLAPGDAYVITTEDANPPLLAQADTTGQGAYFNGNDAVVLNNGPYMIDKIGVIGVDPG
                     ITGWAVGAGSTTDNSLVRKSNVLKGNLDWATAAAEWDVIGPNNFTGLNAHTYDGCGCN
                     APTGISFSAITNASFKVSWGAVSGATKYQVWFRPLGLGTPWSKVNSTATTKKIVGLAP
                     ATTYEVKIKTICGAVTSTFSTIETVLTLPLREGEFEQSLNIYPNPGTGIFTVELPEIV
                     DIASSITIYDLTGKVVMQQTLNTNFDVIQIDATSLSSGNYILRVQTNNSSYTEILVIE
                     "
     gene            complement(1985..2734)
                     /locus_tag="PLB46_12155"
     CDS             complement(1985..2734)
                     /locus_tag="PLB46_12155"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRF77320.1"
                     /translation="MSIHIYPSQKQIKGNFNGGEIMENKPIQMSHDHTKLQPYSNIFY
                     WAHAWAEVTSTIGLHPHQAFEIVSFVLTGTVEHYDTKNKTWLPLQAGDVQIIRAGNGI
                     SHAEKMHEGTHMFQIWFDPNINLSLLQPATYNDYKSSEFPIIEEQGKTIKVLKGEGAP
                     LEMMTPGLSIQQLTLAPQLHTMELNDTHSHAFYVISGQVNTDKGLIDKDDFFIVDEGG
                     TFTFTAEVPTQLFLISALKTLDYTTYAAGNN"
     gene            2874..>3872
                     /locus_tag="PLB46_12160"
     CDS             2874..>3872
                     /locus_tag="PLB46_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77321.1"
                     /translation="MKTKLLLLLTVAVFQLKAQVSTEIYGLYRNNTPPELYLGKVNTA
                     DGSTELLGATLYDSLLVVTGSALNPYTNTYHVLSADRFFAIDNVSGEITDAVTVTLPI
                     ENSYMRSVVFNPSDSGYYGLIIQTNELDASILFGKIDPLTGIVEVLSDTSITQFLMPG
                     RNIINPYEMIYYLLTDSQIIGIDMYTGDIYSTAIITNPYGAQFDNMSFNCKDGVIYGL
                     IPAIDTQLVFLGKLDPFTGVVELVFEAAVDTTYFISGLGTANSAIDPVTNTYYYISAG
                     NLKGIDLADGSITFENSIYDMEGRYFGLFHHVQDCFGAQRTRFATGINTTTLAQQFI"
BASE COUNT         1183 a          681 c          725 g         1283 t
ORIGIN      
        1 cgactttgcc agaggaacta caggaggtaa tatttcaacc ggcggaattt attcattatt
       61 aaccgcagca ggtgccaatc aggtgcttta ttttcaacca actgccgatg attttacacc
      121 gggaactatc acttacgttt ataaaaatat taccggaaca ctgctaactg aacttgattt
      181 ctcctatgtt atatatttat ataataatga aaatcgagct acttcattaa cattatctta
      241 ttcaacagat gatattacct atacaccggt tccatcagtg gattttactt catttgcggc
      301 attagatgtt ttattatata cctttaaccg cagtgtttcc ttaaccggtt tatctattcc
      361 aaataatacc aacatttatt ttaaatggag caccgatgat gttcccggag gtgcaggaaa
      421 ccgcgacgaa attggattag ataatattac attaactgca ggttcagacc cgatacctac
      481 acctactgtt tattttaata atgatattgt ttatgcgaat gaaaatgatg gcagttatgc
      541 tttcgatata aatattgcaa cacctcacga ttgtgatgcc ttcatgacat taaatgcaat
      601 ttcaactgct aaatcaggta tcgattattc ctttacgttc cctgctatgg tttcttttga
      661 agcagccgga cctacaacgc acaccattac aattccttta atagatgatg ccattccgga
      721 atatatggaa accatgtata ttgaattgga tagcgtttca ccgggatgtt taattacggc
      781 tcctgatttt caactggtat ttattgagga cagcgatgtt gcggtaattg gcgattgcga
      841 aaatttaatt atttctcaat atggcgacgg aacaggtttg ttaaataaca aagcgttaga
      901 aatttataat cctacggatg ccagtattaa tttagaagat tattatgtgc acatatatgc
      961 aaatggtgct atttctccaa tttattattt tcaatgtgca ggtatgcttg ctcccggcga
     1021 tgcatacgtg attacaactg aagatgcaaa tccaccatta ttagcacaag cagatactac
     1081 tggtcagggt gcttatttta atggcaacga tgcagtagta ttaaataatg gtccttatat
     1141 gattgacaaa attggtgtga ttggagttga tcctggtata actggttggg ctgttggtgc
     1201 aggaagtaca actgataatt cattggtccg taaatcaaat gtattaaaag gaaatttaga
     1261 ttgggctact gcagctgcag aatgggatgt aataggacca aataatttta caggtttaaa
     1321 tgcacatact tacgatggtt gtggatgtaa tgcacctacc ggaatttctt tttcagcaat
     1381 aacaaatgct tcatttaaag ttagttgggg agcagtttca ggggccacaa aatatcaggt
     1441 atggttccga ccacttggtt taggtacacc ttggtcaaaa gtaaattcaa cggctactac
     1501 taaaaagatt gtaggattgg ctccggcaac tacttatgaa gtgaaaatta aaactatttg
     1561 tggcgcagta acttcaactt tttcaacaat tgaaactgtg cttactttgc ctttacgtga
     1621 aggtgaattc gaacaatcat taaatattta tccaaatccg ggaacgggaa tatttactgt
     1681 tgaattacct gaaatagtag atattgcttc cagcataacc atttatgatt taaccggtaa
     1741 agttgtaatg caacaaacct tgaatacaaa ttttgatgta atacaaatag atgctacttc
     1801 attgtcatca ggtaattata tcttgcgtgt gcagactaat aatagcagtt atactgagat
     1861 attagtaatc gaataaataa aatgatatag taaaaaagga ggttgtctgt aacagataac
     1921 ctcctttttt tttgatgaac aaacagctaa tttttaagca ttgtatttta ttagcatatt
     1981 agtattaatt atttccggct gcatatgttg tataatcaag tgtttttaat gcactgatta
     2041 aaaataattg tgtgggaact tctgcagtaa atgtaaatgt gccgccttca tcaacaataa
     2101 aaaaatcatc tttatcaatt aaacctttat ctgtgttgac ttgtcccgat attacataaa
     2161 atgcatgtga atgggtatcg ttgagttcca tagtatgcaa ttgtggcgct aatgtcaatt
     2221 gctgaatcga caaccccgga gtcatcattt ctaatggtgc gccttcacct tttaacactt
     2281 ttattgtttt tccctgctcc tcaattatcg ggaattcgct cgatttatag tcgttatagg
     2341 tagcaggttg caatagcgac aggttgatat tcgggtcgaa ccagatttga aacatatggg
     2401 tgccttcatg cattttttcg gcatgactaa tcccatttcc tgcgcgaatt atttgcacat
     2461 caccggcttg caacggcagc caggttttat ttttagtgtc gtaatgttca acagtacctg
     2521 ttaacacgaa actcactatt tcaaaagctt ggtgaggatg taaaccaatg gtagaagtta
     2581 cttcagccca ggcatgcgcc caataaaata tattggaata tggctgcaat ttagtgtggt
     2641 catgcgacat ttgaatgggt ttgttttcca tgatttctcc cccattaaaa ttccctttta
     2701 tttgcttttg cgatggatat atatgtatag acatgtaaat gaattatgag ttaaatacaa
     2761 ttagcttatt cgaaaagttc atcatcagcc caaaacatta acatatggaa gcccaaagct
     2821 ggtgaacatt ccctattgtt gcttgtttta actgtcaaca tacaagtatt acgatgaaaa
     2881 caaagttact cctcttactt accgttgctg tatttcaact aaaagcgcag gtaagcaccg
     2941 aaatttacgg actttataga aataatacgc ctcccgaatt gtatttgggc aaagtgaata
     3001 cagcagacgg gtccaccgaa ttgctgggtg ctacgttgta tgattcgtta ctcgtagtaa
     3061 caggctctgc attaaatcct tatacgaata cctaccatgt tttgagcgcg gaccgatttt
     3121 ttgcaattga taatgttagt ggtgaaataa cagatgcggt tactgtaacg ctcccaattg
     3181 aaaacagtta tatgcgttca gtagtattta atccttccga ttcaggttat tacggattaa
     3241 ttattcaaac caacgaatta gatgcatcta ttttgtttgg taaaatagac ccactcaccg
     3301 gaattgtaga agtgttatct gataccagca ttacacaatt tttaatgccg ggaagaaaca
     3361 taatcaaccc atatgaaatg atttattatc tgctcaccga ttcacaaata attggtatcg
     3421 atatgtacac cggtgatatt tattcaactg ctataattac taatccttac ggtgctcagt
     3481 ttgataatat gagttttaat tgtaaggatg gagttattta tggattgata ccggctatag
     3541 atacacaact tgttttcctc ggtaaactgg acccttttac gggtgttgta gaattagttt
     3601 ttgaggctgc cgttgatact acttatttta taagtggatt aggaactgcc aattcagcta
     3661 ttgatccagt taccaatacg tactattata tttccgcagg aaatctgaaa ggtattgacc
     3721 tggcagatgg tagtattact tttgaaaatt ccatttacga tatggaagga agatatttcg
     3781 gtttattcca tcacgtgcag gattgttttg gtgcacaacg tacgcgattt gcaacaggga
     3841 ttaatacaac aactttagcg caacaattta tc
//
LOCUS       DAORKW010000344         3852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1217_length_3852_cov_5.488017, whole genome shotgun sequence.
ACCESSION   DAORKW010000344 DAORKW010000000
VERSION     DAORKW010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1217_length_3852_cov_5.488017"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PLB46_12165"
     CDS             complement(<1..795)
                     /locus_tag="PLB46_12165"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRF77322.1"
                     /translation="MIALQHKTTLHLLRILIGLVFITSAIFKLIGIDAFEVYIFSLQW
                     FNLPLSTVIARLIISAEFIIGIFFITNIQFKWISKITFGLLISFCIFLIIQILSGNKE
                     NCHCFGEIIQLNPTSSLIKNLLLLVILFLIRRDSGFNIKHSNSIALFVIVFAFAFPML
                     YSTPYFAVKWPEMPTNTLEIAAKRAQNNQLLLSQQAASGKKVICFLSVSCSYCVNGAN
                     KIAIIAENNQLNEEILFVFTGDEMLLPEFWEKSNSPRFNYVFLPMRD"
     gene            complement(806..1030)
                     /locus_tag="PLB46_12170"
     CDS             complement(806..1030)
                     /locus_tag="PLB46_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77323.1"
                     /translation="MKLKVGSLVLGVILIGLFSRCDKTFRCDCYQDVMGFDTTYTLVG
                     EGRDEQEVCDNFDSSTTEFGITKTVDCEPY"
     gene            complement(1099..2139)
                     /gene="rlmN"
                     /locus_tag="PLB46_12175"
     CDS             complement(1099..2139)
                     /gene="rlmN"
                     /locus_tag="PLB46_12175"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRF77324.1"
                     /translation="MQATEKISIRDLSLEQLTEQIVALGEPSFRAKQIYEWLWKKSAV
                     QFSEMLNLSKSLREQLESHYTINAIVIDVQQKSMDGTIKCRFKLVDGHMIEGVLIPSE
                     NRMTACVSSQVGCNLGCTFCATGFIKMKRNLEPYEIYDQVVLLNKASLEHHKQPLTNI
                     VFMGMGEPLLNYNNVLAGIAKITGEDGLHISAKRITVSTAGLAKMIRKLADDQVKFNL
                     ALSLHAADDIKRSKLMPINEANNLNDLVKALNYFHDKTGNRITFEYILFDGINESMED
                     AKNLVTLCSKVPARVNIIEYNTIREADFHKSKEDQREKFIAYLETHRVVAKVRRSRGK
                     DIDAACGQLANK"
     gene            2251..3312
                     /locus_tag="PLB46_12180"
     CDS             2251..3312
                     /locus_tag="PLB46_12180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HRF77325.1"
                     /translation="MLQFKSGSGMLRKLTCLLVGLASLSLNAQDIHFSQFFSSPLTLN
                     PALTGAFNGNIRGILNYRNQWNSFAPFNTFATSVDANFGSSFLKNDIMGIGVNAFSDV
                     AGDTRYSSTQVNLTFAYIKTLGDKYHRSYLSAGFQGGYAQKSINYLAMTFANQYNGVY
                     YDPLLGNGESIGFDSKGYLDLSAGLMWYLIPREDKYSMYIGGSMYHVNQPDQSIGAAN
                     DKLYMRSSGHIGAQIPVGEMTSLVPAAMVVFQGPYVEYFAGTNLKFYLQGMKWSSNAI
                     SFGVWQRVVGDTEAATKAESTAIAARIDYDKISLGLSYDVNISSLSEASKNNGGPEIS
                     LTYISSLPQKARKIPCPKF"
     gene            3403..>3852
                     /locus_tag="PLB46_12185"
     CDS             3403..>3852
                     /locus_tag="PLB46_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77326.1"
                     /translation="MHAQTIVLDISKSKLPQGVFIRKDFHLINGDTVYKKFEKYATVI
                     ERGDSVLYLEQFDVNRSWIVSRFLISGKERLPAGWQNEYDINGYKLYDRYCNPENNDC
                     KLYKKYNYYPNGNQMAVITYYHHKLNGNSLFYYNDGKLKHCLEYQKGR"
BASE COUNT         1199 a          680 c          708 g         1265 t
ORIGIN      
        1 atctctcatt ggtagaaaaa cataattgaa acgaggtgaa ttactttttt cccaaaattc
       61 aggcagtaac atttcatctc ccgtaaacac aaataatatt tcttcgttga gctgattgtt
      121 ttcagcaatt atggcaattt tatttgcacc attcacacaa tagctgcacg acacacttaa
      181 aaaacaaata actttttttc ctgacgctgc ctgttgtgat agtaacaact gattattttg
      241 cgcgcgtttg gctgcaattt ccagtgtgtt agtcggcatt tccggccatt ttacagcaaa
      301 atatggtgtt gaatatagca tggggaaagc aaatgcaaat acaattacaa aaagggcaat
      361 ggagttgcta tgtttgatgt taaaaccgct gtccctgcga attaaaaata atatgactag
      421 tagtaataaa tttttaatca gtgatgatgt tggattgagt tgaattattt ctccaaaaca
      481 atggcaattt tctttgttgc cgctaagtat ctgaataatt aaaaaaatac aaaaactaat
      541 tagtagccca aaggttattt tacttatcca cttaaattga atgttagtaa taaaaaatat
      601 accgataata aattccgccg aaataattaa tcgggcaatt acagttgata atggtaaatt
      661 aaaccattgc aaactaaata tgtacacttc aaatgcatca ataccaatca gtttaaaaat
      721 ggcggaggta ataaacacca gtccaattaa aatgcgcagt aaatgtaatg ttgttttgtg
      781 ttgtaatgca atcatgagct actaatcaat agggttcgca gtctactgtt tttgtaatgc
      841 caaactcggt agttgaggag tcgaaattat cacatacttc ttgttcatca cgtccttcgc
      901 caaccagggt ataggtggta tcaaaaccca ttacatcctg ataacaatcg caacggaacg
      961 ttttgtcgca acgggagaat aaaccaataa ggataactcc cagcaccaag ctgccaacct
     1021 ttaatttcat ggtgttaaat taacaatatt gattgaaatg gtaaacacct gtcatttttg
     1081 tgactgcaat atgtccaatt atttattggc taactggcca caagcggcgt caatatcttt
     1141 cccgcgactg cggcgcacct tagctactac gcgatgtgtt tccaaatagg cgataaactt
     1201 ttcacgctgg tcttctttcg atttatggaa gtcggcttcg cgaatggtat tatattcgat
     1261 aatgtttact cttgccggaa ctttactaca taacgtaact aaatttttgg catcttccat
     1321 actctcgttt atgccatcaa acaaaatata ttcaaatgtg atacgattgc cggttttgtc
     1381 gtggaaataa ttcaaggctt taactaaatc atttaaatta ttcgcctcgt taatcggcat
     1441 gagtttcgaa cgtttaatat catcggctgc atgaagcgat agtgcaagat taaatttcac
     1501 ctgatcatca gccaatttgc gtatcatttt tgccaaacct gctgtagaaa cggttattct
     1561 ttttgcagaa atatgcaaac catcttcacc ggttattttt gcaatgcctg ccaatacatt
     1621 attataatta agtaatggct cacccattcc cataaataca atattggtaa gtggttgttt
     1681 gtgatgttca agcgaggctt tgttgagtaa taccacctgg tcgtatattt cgtatggttc
     1741 cagattgcgt ttcattttaa taaaaccggt agcgcagaat gtacatccca aattacatcc
     1801 aacctggctc gacacacatg cagtcatcct gttttctgat ggaattagta caccttcaat
     1861 catgtgtccg tcaactaatt taaaacgaca tttaatggtg ccatccatac ttttttgctg
     1921 aacatctatt acaattgcat taatagtata atggctttct aattgctcgc gaagtgattt
     1981 tgataaattg agcatttcgg aaaattgcac ggcacttttt ttccaaagcc attcataaat
     2041 ttgtttagcg cggaaggaag gttctcccaa tgctactatt tgctcagtga gttgttcgag
     2101 acttaaatcg cggattgata tcttttctgt tgcctgcatt tatgcaaagt taaactttcc
     2161 aaagggttac tgctgctttt tccggcgggt ctttaataac agggcctttg acggcttaat
     2221 tttgaaaatt gcggtaaaaa gcggaacttc atgcttcaat ttaagtcggg ttcaggtatg
     2281 ttaagaaaac tcacatgttt acttgttggt ttagcctcgt tgtcgttaaa cgcgcaggat
     2341 attcatttta gtcagttttt ttcctccccg cttaccctta atcctgcact tacaggtgct
     2401 tttaacggga atatccgtgg tattcttaat tatcgcaatc aatggaactc atttgcacct
     2461 tttaatacgt ttgccacaag cgtagatgcc aattttggtt cttcattttt gaaaaatgac
     2521 attatgggaa taggtgtaaa cgcattttca gatgttgcag gcgacacccg atatagtagc
     2581 actcaggtaa atttaacctt tgcctatatc aaaacattag gcgataaata tcacagaagt
     2641 tatttatcag caggttttca gggaggatat gcgcaaaaaa gtattaatta tctggccatg
     2701 accttcgcca accaatataa tggtgtttat tatgatccgc tgttgggtaa tggagaatcg
     2761 attgggtttg actccaaggg ttatttggat ttatctgccg gtttaatgtg gtatctcatt
     2821 ccaagagaag ataaatacag catgtatatc ggtggttcca tgtaccatgt aaatcaacct
     2881 gaccagtcaa ttggagccgc aaacgataaa ttatatatgc gcagctcagg tcatattgga
     2941 gcacaaattc cagttggaga aatgacatca ttggtacctg ccgctatggt ggtatttcaa
     3001 gggccttatg tggagtattt tgcaggcaca aacttgaaat tttacttaca aggcatgaaa
     3061 tggagttcta atgccatttc ttttggcgtt tggcaaaggg tagttggcga tactgaagct
     3121 gctacaaaag cagaatctac agctattgca gcaagaattg attatgataa aataagttta
     3181 gggttgagtt atgatgttaa tatatcatca ctttctgaag ctagcaaaaa taatggaggg
     3241 ccggaaattt cacttaccta tatttccagt ttaccacaaa aggcaagaaa aattccttgt
     3301 cctaaatttt aatttcgggc tatactaatc taataactga aatacattat tagcatgaat
     3361 aaattttgta tactgatatt ttgcttttgt ttgatgtcag ttatgcatgc ccaaactatt
     3421 gtattagata tctccaaaag taaactacct caaggtgtat ttattcggaa agattttcat
     3481 cttataaatg gtgataccgt gtataaaaaa ttcgaaaaat atgccaccgt aattgaacgt
     3541 ggtgattccg ttttatatct tgaacaattt gatgttaacc ggagttggat tgtatcacgt
     3601 tttttaatta gtggtaaaga aagattgcct gcaggatggc aaaatgaata tgatattaat
     3661 ggatataaat tgtatgatcg ttattgcaat cctgaaaaca atgattgtaa attgtataaa
     3721 aaatataatt attaccccaa tggcaatcaa atggcggtaa ttacgtatta tcatcataaa
     3781 ttaaatggca acagtttatt ttattataac gatggtaaat taaaacactg tctggaatat
     3841 cagaaaggca gg
//
LOCUS       DAORKW010000345         3849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1220_length_3849_cov_3.976542, whole genome shotgun sequence.
ACCESSION   DAORKW010000345 DAORKW010000000
VERSION     DAORKW010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1220_length_3849_cov_3.976542"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1462
                     /locus_tag="PLB46_12190"
     CDS             89..1462
                     /locus_tag="PLB46_12190"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRF77327.1"
                     /translation="MKKIISVLTLALLITVTVSAQTKLPSNFFMKKLPNGLEMLIIED
                     KTVPLATIEIVVKNGSYTEDSAFNGLSHMYEHMFFKANKDLPSQEAFLKRINELGIVF
                     NGTTSDERVNYFITLTNNKVDEGIAFMNSAIRYPLFLEEEMKKENPVVDGEFQRAESN
                     PTFFLFDEFNRYMWKENYYRKNPIGVHDVILTCTPEKMRIIQDKYYYPNNSMIVIAGD
                     VNHQEMFTKIEKVFGDWKPADFDPFQKWPIPEFEPLKGVTNFVTMNENAQVPIFLTGF
                     HGPDTRNDLKATYAADVFSYILSQASSKLQKDLVESGLAYQVNVGYQTCKYTGPINII
                     LVPNPATINEAWKVLWENVAQWTDPNYYTDEQLETAKNLLAIQDAYGKESTSEFTHYV
                     TYWWASATIDYYTNYVQNLQEVTRQDITNYVKKYIIDQPHVNGLLLNPSMKDMVDVKS
                     IGFEIKE"
     gene            1490..2818
                     /locus_tag="PLB46_12195"
     CDS             1490..2818
                     /locus_tag="PLB46_12195"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRF77328.1"
                     /translation="MKNIKIAFLLVATVLTSTLVSAQGTKEYTVDGIKVIHKYVPKDV
                     ISVRLFVEGGTANYSKEKQGIENLTLNLMVDGGTTTMPKLDFKTASEKIGTSFGASTA
                     LDYGSISMTCIKAFWDQSWNLFSDAILNPAFSENEYNLLKEQLIANAQQTMADPDGSL
                     DLLARQIAFENKDYAKAYDGTPESLAGISLDDVKNYYKKTLGKKRTFLVVVGNITEED
                     LTAKIKASLGKMANGTPVSKESRTLITQSKIHVEDRDIATNYIMGIMSAPFANTEEGI
                     NYRIGMSIMGDRYFTELRTKRSLSYAPAAFYNRGAVTNPYGAIYITTIDPKQSMQVMV
                     DELDKVKKEGFTETEFTDKKQEFLTGYYLTLETTSNQADALGRSEISGGWEALDQLTE
                     NVNKAKLADINATFDKYSDVIVWIYLGKKDAVKTEDFKQTTPKEKKSKPY"
     gene            complement(3399..>3849)
                     /locus_tag="PLB46_12200"
     CDS             complement(3399..>3849)
                     /locus_tag="PLB46_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77329.1"
                     /translation="SKLDERKEQLKTLIFNPGQPVDKIPLIKNKMEIFSPEMQPFYDY
                     AIAQKKYSTGVDCYVFSIEKKQDAIKGEDVVINELVTWFDKKSMQIVARHYSLSYFTR
                     LFDFDVVMDVKLSTINGVQVPVSISYNGYWDIPARKPEIGSVQINIW"
BASE COUNT         1326 a          693 c          672 g         1158 t
ORIGIN      
        1 aatgccattt aacatagttt aaccggcggt ttttaggcac ctaaaatgca aactactacc
       61 tttacggcaa caatatcaat aattgtctat gaaaaaaata atcagtgttt taaccctcgc
      121 gttgttgatt acggttaccg tatcagcaca aaccaagcta ccatccaatt tctttatgaa
      181 gaaactgcca aatggtttgg aaatgctcat cattgaagac aaaaccgtac cactggccac
      241 tatcgaaatt gtggttaaaa acggttctta taccgaagac agcgctttca acggtttgtc
      301 acacatgtat gaacatatgt tttttaaagc aaataaagac ctgcctagtc aggaagcctt
      361 tttaaaacgt atcaacgaat tagggatcgt atttaatggc acaaccagcg atgaacgtgt
      421 aaactatttt atcacgctta ccaataataa agttgatgaa ggaattgcct ttatgaattc
      481 tgccattcgt tatcctttat ttttagagga agaaatgaaa aaagaaaatc cggtggttga
      541 tggtgaattt caacgcgctg aatctaaccc taccttcttt ttatttgatg aatttaatcg
      601 ctacatgtgg aaagaaaact attatcgcaa aaacccaatc ggggtacatg atgtaattct
      661 tacatgtaca cctgaaaaaa tgcgtattat tcaggataaa tattattacc ctaacaatag
      721 tatgattgta attgccggtg atgttaatca tcaggaaatg tttaccaaaa ttgaaaaagt
      781 ttttggcgac tggaaacctg ctgatttcga tccattccaa aaatggccga tacctgaatt
      841 tgaaccgtta aaaggtgtaa cgaactttgt taccatgaac gaaaatgcac aggtgccgat
      901 tttcttaacc ggatttcacg gtcctgatac ccgcaacgat ttaaaagcaa cttatgctgc
      961 tgatgtattc tcttacattc tttcacaggc ttcaagcaaa ttgcaaaaag atttagtaga
     1021 atcaggttta gcttatcagg taaatgttgg ttatcaaacc tgcaaataca caggtcctat
     1081 taatattatt ttagtgccaa atccggcaac tattaatgaa gcctggaaag tgttatggga
     1141 aaatgttgct caatggactg accctaatta ctacactgat gagcaattag aaacagctaa
     1201 aaatttatta gctattcaag atgcatatgg taaagaaagc accagtgaat ttacacacta
     1261 tgttacgtat tggtgggctt ctgcaacgat agattattat actaactatg tgcaaaattt
     1321 acaggaagtt accagacagg atataacgaa ttatgtaaaa aaatacatta ttgaccaacc
     1381 tcatgtaaac ggattattat taaatccttc tatgaaagat atggttgacg taaaatctat
     1441 tggtttcgaa attaaagaat aattacccta atcgctaaat taaaacgata tgaaaaatat
     1501 taaaatagct tttttactgg tagccacggt gttgacttca acattagtat ctgcacaggg
     1561 aacaaaagaa tataccgttg atggtattaa agtaattcat aaatacgtac ctaaagatgt
     1621 aatcagtgta cgtttatttg ttgaaggtgg aactgccaac tatagcaaag aaaaacaagg
     1681 tatcgaaaac cttaccttga atttaatggt agatggtggc acaaccacaa tgcctaaact
     1741 cgatttcaaa actgcttcag aaaaaatcgg aacatcgttt ggtgcaagca ctgcattaga
     1801 ttatggttca atcagcatga cttgtattaa agcattttgg gatcaatcat ggaatttatt
     1861 ttctgatgcg attttaaatc ctgcattttc tgaaaatgaa tacaatttat tgaaagaaca
     1921 attgattgcc aatgcacaac aaactatggc agacccggat ggttcattag atttattagc
     1981 gcgtcagatt gcatttgaaa ataaagatta tgcaaaagct tatgatggca caccggaaag
     2041 tctggccggt atttcattgg atgatgtaaa aaattactac aaaaaaacat taggtaaaaa
     2101 acgtacattt ttagttgtag ttggtaacat tactgaagaa gatttaacag caaaaattaa
     2161 agcttcatta ggcaaaatgg ccaacggcac tccggtttct aaagaatcgc gtacgttgat
     2221 tacacaatct aaaattcatg ttgaagaccg tgatattgct accaattata ttatgggtat
     2281 tatgagtgct ccatttgcaa atacggaaga aggtattaac taccgcattg gtatgtcaat
     2341 tatgggtgat cgttatttca ctgaattaag aaccaaacgc agcttaagtt atgctcctgc
     2401 tgctttttat aaccgtggtg cagttactaa cccttatggt gctatttata tcaccaccat
     2461 tgaccctaaa caaagtatgc aggtaatggt ggatgaatta gataaggtga aaaaagaagg
     2521 ttttacggaa actgagttta ccgataaaaa acaggagttt ttaaccggtt attatcttac
     2581 acttgaaact acatctaatc aggctgatgc tttaggtcgc tctgaaatta gtggtggatg
     2641 ggaagcattg gaccaattaa cagaaaatgt aaacaaagct aagttagctg acataaatgc
     2701 tacatttgat aaatacagcg atgtaattgt ttggatttat ttgggtaaaa aggatgcggt
     2761 gaaaaccgag gactttaaac aaacaacacc taaagagaaa aaaagcaaac cttattaatt
     2821 gttagcacaa ctatttcaaa aaccgcattt atttaagtgc ggtttttttt atgcctaaac
     2881 acgattgtga ggtgcatact taatttgtgg ttcacccgcc tcagcggact gaaagcgcgg
     2941 agaaaggaga ggcggagtgc ggaatagatt atgttgtttc tcgcaaagac ccaaagtaat
     3001 taatatgtta ttttaagtct aaatataaaa ccaattcaag ctagcaaagg tacatggttt
     3061 aacagctttg cgcgcttgaa aacataccat atcgcacaac ctacaacacg aaatacttag
     3121 cgactttgcg agaaaaaatt tggtttacca actccgcgtt ctcaaaaaca aaccacattg
     3181 cacatcatat attcctataa tccccgcgac tccgcgtgaa ataaattatg tttgtttctc
     3241 gcaaagaccc aaagtaatta atatgttatt ttaagtctaa atataaaacc aattcaagct
     3301 agcaaaggta catggtttaa cagcttacgc gcttgaaaac ataccatatc gcacaaccta
     3361 caacacgaaa tacttagcgt ctttgcgaga aacacctact accaaatatt gatttgcacg
     3421 ctccctattt caggtttgcg ggcaggaata tcccaatacc cattataact tattgagaca
     3481 ggcacctgaa ccccattaat tgtagacaat tttacatcca ttaccacatc aaaatcaaat
     3541 agacgagtga aataactcaa actataatgc cgcgccacta tttgcatcga ttttttatcg
     3601 aaccaggtta ccagttcatt aattaccaca tcctctcctt taatagcatc ctgttttttt
     3661 tcaatactaa atacataaca atccacgccg gtgctgtatt ttttttgtgc tatcgcataa
     3721 tcataaaaag gttgcatttc gggactaaat atttccattt tgtttttaat caatggaatt
     3781 ttatccaccg gttgtcccgg attaaatatt aaagttttta attgctcctt tcgttcatcc
     3841 agtttcgaa
//
LOCUS       DAORKW010000346         3847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1222_length_3847_cov_5.377901, whole genome shotgun sequence.
ACCESSION   DAORKW010000346 DAORKW010000000
VERSION     DAORKW010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1222_length_3847_cov_5.377901"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLB46_12205"
     CDS             <1..301
                     /locus_tag="PLB46_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77330.1"
                     /translation="DPLFFPAIDHQLFLELDYKCDIPFNIWVKCNTATGSALYDEVLT
                     ITSKENWNKIYVNLNATLQYFAAYQPESYHLEFRAINTLDTATIMFDNIKIIQSK"
     gene            301..1725
                     /locus_tag="PLB46_12210"
     CDS             301..1725
                     /locus_tag="PLB46_12210"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRF77331.1"
                     /translation="MRFKKKIGILTYILLDYFAAMLVWAGLFFYRKFVIEDLPFDITN
                     KVLNDKNFFYGIFAIPIIWIVVHFIGGTYTDIYRKSRLAELTRTFVVSFIGVLIIFFA
                     LLLDDYISDYRDYYQIFFLLFFGQFLLTVLFRIIILTRAKRQLQKGVVAYNTIIIGSD
                     LRASSIHQEITHYKKSLGYAFVGYIEANGNHGSDLDKFMPCLGNLSQLNEIINARNID
                     EVIIAIETSEHHLLNEIIDKLAEKGNIVIKIVPDMYDILSGSVKMGNVLGAVLIEIYP
                     DLMPRWQRIIKRGIDIAVAIFVILILWPLYLFIAIKVKFSSKGPIFYYQQRVGINSKP
                     FTIIKFRSMYVTAEDNGPALSSENDERITTWGKVMRKWRFDELPQIFNILKGEMSLVG
                     PRPERQFYIDQITKIAPSYKHLQKVKPGLTSWGMVKFGYASNVDQMVQRMKYDLLYIE
                     NMSLAIDFKIMIYTVLIIFQGKGK"
     gene            1809..>3847
                     /locus_tag="PLB46_12215"
     CDS             1809..>3847
                     /locus_tag="PLB46_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRF77332.1"
                     /translation="MNIPPGLSEKEVIAMRQQFGANGITGKRQNKLWHLLIGVVAEPM
                     FIILVFAAIIYFILGESAEGFIMLAALFFVAGISIYQENRSSNAVEALKQLTDSGAKV
                     ARSGKTIIVPTNDIVVNDIIFCEDGNIVPADAELLEAHDFSVNESMLTGESVPVFKAP
                     GGDPVLIFKGTLVATGSCIAKVTRVGVQTEMGKIGKSLSAIETSKTPLQIQISAFVLH
                     MVIAGVIAFFIVWGINYYTTGSLLHGLLHGLTMAMSVLPEEIPVAFSTFLALGAFRLI
                     KKNVIARTPQTVEALGAATIICADKTGTLTENSMQVAGVYILESDSYFSYIENPAQPN
                     IVLEYAMWSSEVSPFDPMEKSIHVLYTNSYTTDKRLEYKMVHEYPLAGTPPIMTHVFR
                     NAANDIIIAAKGGVESILRQSTLTEQQKEKVISTTQQLSSQGYRVLAVAKSANNYTSF
                     PVAQSELTFTFLGLIAFNDPPKKNTASIVQSFYKAGIGVKMITGDYAPTAVAIAQQIG
                     LKNHATVLTGNEIMEMSDETIAQKVRDVDVYARMFPEAKLKVVEAYKHNGEIVAMTGD
                     GVNDGPALKAAHIGIAMGQRGTEIAKQAASLILTDDNLQHMVEAVALGRRIYENLKKA
                     IRYIISIHIPIILIVTIPLLFFWDFTDFFNPIHVIMLELIMGPTCSIIFENEPADT"
BASE COUNT         1258 a          646 c          713 g         1230 t
ORIGIN      
        1 cgacccatta ttttttccgg ctattgacca tcagttattt ttagagttag attacaaatg
       61 cgatattcct ttcaatattt gggtaaaatg taataccgca accggctcag ctttgtacga
      121 cgaggtgctt accattactt ctaaagaaaa ctggaacaaa atatatgtga atttaaatgc
      181 cactttacaa tattttgcag cttatcaacc agagtcttat catttagaat tcagagctat
      241 taacacgctg gacacagcaa caattatgtt tgataatatt aaaattatac aatcaaaata
      301 atgcgattta aaaagaaaat tggcatcctt acctatatcc tgctcgatta tttcgcggca
      361 atgctggtat gggctgggtt atttttctat cgcaaatttg ttattgaaga tttacctttt
      421 gatattacga ataaagtact caacgataaa aactttttct acggcatttt tgccattcct
      481 attatttgga tagtggtaca ttttattggc ggaacctata ccgacattta ccgtaaatcg
      541 cgtttagcag aattaacacg cacctttgtg gtttcgttta taggtgtatt aattattttc
      601 tttgccttac tacttgatga ttatattagc gactaccgcg actattatca aatattcttt
      661 ttattgtttt tcgggcaatt tcttttaacc gtattgtttc ggattatcat attaaccaga
      721 gcaaaacgtc aattgcaaaa aggtgtagtg gcttataata ccattataat tgggagcgat
      781 ttacgcgcat cgtctataca ccaggaaata acccattaca aaaaatcatt aggctatgca
      841 tttgtaggtt acatcgaagc aaatggcaac catggttctg atttagataa atttatgcca
      901 tgtttaggta atttatcgca attaaatgaa attataaatg cccgcaatat tgatgaagtt
      961 attattgcga tagaaacttc tgaacaccat ttattaaatg aaataattga taaactggca
     1021 gaaaaaggta atatcgtgat taaaattgta cccgatatgt atgatatatt atcaggaagt
     1081 gtaaaaatgg gtaatgtatt aggggcagta ttaatcgaaa tttatcctga cctgatgcca
     1141 cgttggcagc ggattattaa acgtggaatc gatatcgcag ttgccatttt tgttatactt
     1201 atattatggc ctttgtattt gtttatagct ataaaagtca aattctcatc caaaggacca
     1261 atattttatt atcaacagcg agtaggtatt aatagcaaac cattcactat cataaaattc
     1321 aggtctatgt atgtaactgc tgaagataac ggaccagcct taagtagtga aaatgatgag
     1381 cggattacaa cctggggtaa agtaatgcgc aaatggcgat ttgatgaatt acctcaaata
     1441 tttaatatcc ttaaaggcga aatgagttta gttgggccgc gacctgaacg acaattttat
     1501 atagaccaaa taaccaaaat tgccccttcc tataaacatc ttcaaaaagt aaaacccggc
     1561 ttaaccagtt ggggtatggt aaagtttggt tatgccagta atgttgacca aatggtacaa
     1621 cgtatgaaat acgatttatt atatatcgaa aacatgagtc tggctattga ttttaaaatt
     1681 atgatttata cagtgctcat catatttcag gggaagggta aataacttca tggattttta
     1741 ctgattttaa tcatcttaac actacatatc aggtattacc tttataatgc ttaatttagg
     1801 cattaacaat gaatatccca ccgggtttat ccgaaaaaga agtaattgct atgcgccagc
     1861 agtttggcgc taacggcatc acaggtaaac gtcaaaataa attatggcat ttacttatag
     1921 gcgtagtagc agagcctatg tttattattt tagtatttgc agctattatt tattttattt
     1981 taggcgaatc ggctgaagga tttattatgt tagcggcttt attttttgta gccggtattt
     2041 ccatatatca ggaaaacaga agcagtaatg cggtagaagc tctcaaacaa ttaacagata
     2101 gtggggctaa agttgctcgc tcaggaaaaa caataatagt tcctacaaat gatattgtgg
     2161 taaatgatat catattttgt gaagatggca atattgttcc tgcagatgct gaattattag
     2221 aagcacatga tttttcggtg aatgagagca tgttgacagg tgagtcggtt cctgtattta
     2281 aagcaccggg aggtgaccct gttttgatat ttaaaggaac attagtggca acaggttcat
     2341 gcatagcaaa agtaacgcgt gttggtgtgc aaaccgaaat gggtaaaata ggaaaaagtt
     2401 tatctgcaat tgaaaccagc aaaacacctt tgcaaataca aataagcgca tttgtattac
     2461 acatggtgat tgccggtgtt attgcctttt ttatagtgtg gggaattaat tattatacaa
     2521 ccggtagctt attgcacggt ttattacacg gattaactat ggccatgtcg gttttacctg
     2581 aagaaattcc tgttgcattc agtacgtttt tagcattagg cgcatttcgt ttaattaaaa
     2641 aaaatgtgat tgctcgcaca ccacaaactg ttgaggcttt aggtgctgct acaataatat
     2701 gcgcagataa aaccggcaca ttaactgaaa atagtatgca ggttgccggc gtttatattt
     2761 tagaaagcga cagttatttt tcttatatcg aaaatcctgc ccaaccaaat attgttttag
     2821 aatatgccat gtggtcatcg gaagtttcac cttttgaccc gatggaaaaa tcaattcatg
     2881 ttttatatac taattcatat acaaccgata aacgattaga atacaaaatg gtgcatgaat
     2941 atccacttgc gggtacacca ccaattatga cacatgtttt tcgaaatgca gctaacgata
     3001 ttataatagc tgcgaaaggt ggtgttgaaa gtatattacg ccagtcaacc ttaaccgaac
     3061 aacaaaaaga aaaggttatt tcaaccactc aacaattatc atcacaaggc taccgtgttt
     3121 tagcagtagc aaaatctgcc aataattata caagtttccc ggttgcacaa tctgaattaa
     3181 catttacatt tttaggatta attgcattta acgatcctcc taaaaaaaat actgcatcca
     3241 ttgttcaatc attttataaa gccggtattg gggtaaaaat gataaccggc gattacgccc
     3301 caacagcagt ggctattgct caacaaattg gtttaaaaaa tcatgctact gttttaactg
     3361 gaaatgaaat aatggaaatg agcgacgaaa caattgcaca aaaagtacgt gatgttgatg
     3421 tgtatgcacg catgtttccc gaagcaaaat taaaagtggt ggaagcatat aaacacaacg
     3481 gagaaattgt agccatgaca ggcgatggcg taaacgatgg ccctgcctta aaagctgcgc
     3541 atattggtat tgcgatgggg cagcgaggaa ctgaaattgc caagcaagct gcctcattaa
     3601 ttctaaccga tgataatctt caacacatgg ttgaagccgt agctttgggg agaaggatat
     3661 atgaaaatct taaaaaagcc attcgttata ttattagcat tcatattccc attattctga
     3721 tagttacaat acctctatta tttttttggg actttaccga tttcttcaac cccattcacg
     3781 taattatgct ggaattgatt atggggccta cctgttccat tatttttgaa aatgaacctg
     3841 cagatac
//
LOCUS       DAORKW010000347         3845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1225_length_3845_cov_5.865699, whole genome shotgun sequence.
ACCESSION   DAORKW010000347 DAORKW010000000
VERSION     DAORKW010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1225_length_3845_cov_5.865699"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /gene="bla"
                     /locus_tag="PLB46_12220"
     CDS             <1..734
                     /gene="bla"
                     /locus_tag="PLB46_12220"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BlaB/IND/MUS family subclass B1
                     metallo-beta-lactamase"
                     /protein_id="HRF77333.1"
                     /translation="FNAMRCILIVFIGLLSGLVQAQQLEISPLTTNFYVYTTKKVMDG
                     EVVPSNSMYLVTNEGVVLFDTPWDSTQLQPLLDTIMARHNKKVLMSISTHYHADRTEG
                     LTYLASKGIKTYSYLKTYELGKLKDEELAEHIIYSDTTFKIGNYTFETFYPGAGHTPD
                     NIVVWFPLQKVLYGGCFVKSTKSPSIGYIGDASMSSWKRSVKKVEKKYKHPEYIIPGH
                     YSWESTKSLKYTKKLIRKYNRSNKL"
     gene            851..1303
                     /locus_tag="PLB46_12225"
     CDS             851..1303
                     /locus_tag="PLB46_12225"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF77334.1"
                     /translation="MHVVTPVLWFNGKVAEAIEFYCSVFKNAEVLHKSYYPEGEVLTA
                     SMKIEGQKINFLNGGPKFSLTPAISFMVNCKNQEEVDYYWNALTTGGEESMCGWLVDK
                     FGLSWQIIPDALGKLLNDPDKNKAQKVMTAMLKMKKIIVADLEAAHQN"
     gene            1411..>3845
                     /locus_tag="PLB46_12230"
     CDS             1411..>3845
                     /locus_tag="PLB46_12230"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HRF77335.1"
                     /translation="MKCFRLTFICIICILGLRLHAQEFPAITYPFQTIEWENNSTITP
                     VADLCLDEAGYLYIATKEGVFIWDGNRMRNIAHDPDDSASLISNDVNAIISAGKNKIA
                     IITNSGLAILDTKTNTLYQTNSKDLSANGFNGIKYVEIDNKNQLWLANEKNIVVYNTD
                     TKVYTPFKLMDGKLLNNEYIIDVFADKNEIYFLTSYHLYVYAILNNTLQLVPFPLENE
                     EMVSFTITATKILILNTSGNLYQFSKKSLELIQSNFLSAHISPYGSYPIVEVSEDEFW
                     ITSSLNKLNYIISGNTVNQLQLSGISDNIYAKTILKSPSGDLYFGCVNNVKYIPAKLI
                     QSKTIPFTRHQKAMERRMPKNIVVKDSTIFSIDRNWFFYEHHIPSDKIITNNKLPDLI
                     LKLYMPAVYIHPINDTAYLTGFANNDFPQSNLNKTTLINNKLSYFFYYNTKRQQFSTA
                     DFNTSFYNRAKGKYQYDAFNTNDSSIYFIKNNHIEWHHFTNKTTKTIKFSVDSSLYSQ
                     LFYAIYLDADGNFWTASVHDTSNPKKTKSGCLVKYHYPTGKTTVYPADKGISFASSIV
                     LDMTCDTKGNVYLLNKFGVCQLNSDSGKTSLFYSNQLLPDNTYVSMELDAKNRLWLGT
                     LTNGLVVYDIPTNKYILLKGQPTLLSNDMLMQASTQDDNNHLYFWDVTGINIFNSNDI
                     QFNLPQPKAIVSDILLRGKSIIGNKLIDDLAAYVFPHNNNDIDFAFSLLSFNESSKNT
                     FAYMLEGVDEKWIYADTRNFASYHNLSPGKYTFLVKGKNNYGIWSEQPAKVIIEITPA
                     FWQTVWFKLLCTLL"
BASE COUNT         1319 a          651 c          652 g         1223 t
ORIGIN      
        1 aatttaatgc gatgagatgt atattaatag tttttatagg cttgttatcc ggtttagtgc
       61 aggcccagca gctggaaatt agtcccctta caaccaattt ttacgtgtat accaccaaaa
      121 aagtaatgga tggtgaagtt gttccatcaa acagcatgta tttggtaacg aatgaaggtg
      181 ttgtcttatt tgacacaccg tgggactcta cgcaattgca gccattgctg gataccatca
      241 tggctcggca caataaaaaa gtgttaatga gcatttctac ccattaccat gctgatagaa
      301 ccgaaggatt aacttacttg gcatcaaaag gaataaaaac atattcatat ttgaaaacgt
      361 atgagctggg caaattgaaa gatgaagaat tagcagaaca tattatttat agcgacacaa
      421 cttttaaaat tggcaattac acgtttgaaa cattttatcc cggtgcaggt catacacctg
      481 ataatattgt ggtttggttt ccgctgcaaa aagtgttgta tggaggttgt tttgtaaaaa
      541 gtactaaatc gccatctatt ggttatatcg gtgatgccag catgtcgtct tggaaacgct
      601 cagtgaagaa ggttgaaaaa aagtataaac atcctgaata tattattccc ggtcattata
      661 gttgggaaag taccaaaagt ttaaagtata caaaaaaatt aattcgtaaa tacaatcggt
      721 caaataagtt gtaatagtac tattccttcc aactttgtcg caataacacc tatatttagc
      781 cgtatttccc tgccattagg tgattattgg atattaactt tgtttttttc atgtttctaa
      841 atttggtgtt atgcacgtag ttactcctgt attatggttc aatggtaaag ttgctgaggc
      901 aattgaattt tattgttcag tttttaaaaa tgcagaggtt ttgcataaat cgtattatcc
      961 tgaaggagaa gtgctgacag cttcgatgaa aattgaaggg caaaaaatta attttctaaa
     1021 cggtggtcca aaattttcat taactcccgc catttcgttt atggtaaatt gcaaaaatca
     1081 agaagaagta gattactatt ggaatgcttt aactaccggt ggcgaagaat ccatgtgtgg
     1141 ttggttggta gataaattcg gcttgtcgtg gcaaattatt ccggatgcat taggtaaatt
     1201 gcttaacgat cccgataaaa ataaagcaca aaaagtaatg acggcgatgc tgaaaatgaa
     1261 aaaaattatt gtagccgacc tggaagctgc ccaccaaaat taattatcag tgaactatta
     1321 gggcattaat tgtctgtacc tacaccggtt ttagccaaat ttgttatttt tgagtaatta
     1381 ggctgaattc ttttactcaa aggctgcttc atgaaatgtt ttaggctaac atttatttgt
     1441 attatctgca ttttaggctt gcgactgcat gctcaggaat ttccggcgat aacttatcca
     1501 tttcaaacta ttgaatggga aaataatagc accataaccc ctgtcgcaga tttatgttta
     1561 gatgaagccg gttacttgta tattgctacc aaagaaggtg tttttatttg ggatggaaac
     1621 agaatgcgca atatagccca cgaccctgat gatagtgcaa gtttaatcag caacgacgta
     1681 aatgcgataa taagtgcagg aaaaaataaa attgccataa ttaccaattc cggattggca
     1741 atattagata ccaaaaccaa tacgctttac caaacaaatt ccaaagattt atcagccaat
     1801 ggttttaacg gaattaaata cgttgaaatt gacaataaaa atcaattatg gctggcaaat
     1861 gaaaaaaata tcgtggttta taacaccgat acaaaagttt ataccccatt taaactgatg
     1921 gatggcaaac tcttaaacaa tgaatatatt attgatgtat ttgccgataa aaatgaaatt
     1981 tatttcctca cttcctacca tttatacgtt tatgcaattc taaacaatac gttgcaatta
     2041 gtaccgtttc ctttggaaaa cgaggaaatg gtgagcttta ccattactgc tactaaaata
     2101 ttaatactaa atacaagcgg taatttgtat cagtttagta aaaaaagtct tgagttaatt
     2161 caatctaatt tcctttcagc gcatatatca ccatatggct cctacccgat tgtagaagtg
     2221 agtgaagatg aattttggat tacatcaagc ctcaataaac tcaattacat tatttcaggc
     2281 aacaccgtta accaattaca attgagtggc attagcgata acatttatgc aaaaacgatt
     2341 ttaaaatcac catccggcga cctttatttt ggttgcgtta acaatgtaaa atacataccc
     2401 gctaaattaa ttcaaagcaa aaccattcca tttacgcgtc atcaaaaagc aatggagcgg
     2461 cgcatgccaa aaaacattgt tgttaaagat agtacaatat tcagtatcga taggaattgg
     2521 tttttttacg aacatcacat tccttctgat aaaataatta ctaacaacaa actgcctgat
     2581 ttaattctta aactgtatat gcccgcagtt tatatacacc caataaatga cacagcgtat
     2641 cttaccggtt ttgccaataa tgactttcca caaagtaatt tgaataaaac aacattaatt
     2701 aataataaat tatcctactt tttttattat aatacaaaaa gacaacagtt ttccacagca
     2761 gactttaata cttcatttta taatcgggca aaaggtaaat accaatatga tgcctttaat
     2821 accaatgaca gctccattta ttttataaaa aacaatcaca ttgaatggca tcatttcaca
     2881 aataaaacaa caaaaacaat taaattttca gttgacagtt ccctttatag tcaattattc
     2941 tacgccattt accttgatgc tgatggtaat ttttggacag cttcggtgca cgacacgagc
     3001 aaccctaaaa aaacaaaatc aggatgtttg gtaaaatatc attacccaac cggtaaaaca
     3061 acggtatacc ctgccgacaa aggcatttct tttgcttcga gtattgtgtt ggatatgact
     3121 tgtgatacta aaggaaatgt ttatttactg aataaatttg gtgtttgcca attgaattct
     3181 gacagcggta aaacctcgtt attttatagc aatcagttat tgccggataa tacatatgtg
     3241 tcaatggaat tagatgccaa aaatcgattg tggttaggca cattaacaaa tggtttagtt
     3301 gtatatgata tcccaaccaa caaatacatc cttttaaaag gtcagccaac cttattatca
     3361 aatgatatgc tgatgcaagc cagcacacag gacgataata atcatttata tttttgggat
     3421 gttaccggaa ttaatatttt taattcgaat gatattcagt ttaatcttcc acaacccaaa
     3481 gcaattgtat cagatattct gttgcgaggt aaaagcataa taggaaataa attaattgac
     3541 gaccttgctg cctatgtttt tcctcataat aataatgata ttgattttgc cttttcacta
     3601 ttgagtttta acgaatcatc taaaaatact ttcgcctaca tgctggaagg tgtcgacgaa
     3661 aaatggattt atgccgacac ccgaaatttt gcttcttatc ataatttaag tccgggtaaa
     3721 tatacttttt tagtaaaggg aaaaaataat tatggtattt ggagcgaaca acctgccaaa
     3781 gttattattg aaataacacc ggcattttgg caaacagtat ggtttaaatt attatgcacc
     3841 ctact
//
LOCUS       DAORKW010000348         3844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1227_length_3844_cov_4.615202, whole genome shotgun sequence.
ACCESSION   DAORKW010000348 DAORKW010000000
VERSION     DAORKW010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1227_length_3844_cov_4.615202"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLB46_12235"
     CDS             complement(<1..444)
                     /locus_tag="PLB46_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77336.1"
                     /translation="MNDKKYQAQLALVEAAKAKKRAFVLNCKHVLTEQHFLDTDGLFM
                     DNFEHATLVPQSFIYEPDFILQLDNLQLYCVLLAASPDNPEVGYFGHTEHLFGYLQTT
                     IDYGHILITPKSSEQKLVSFFIQQHSKYTTDKQFLKKFQVEEANEN"
     gene            complement(477..1670)
                     /locus_tag="PLB46_12240"
     CDS             complement(477..1670)
                     /locus_tag="PLB46_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77337.1"
                     /translation="MIKSIRDQYNKAFTEADYQKFVHACQDKYDKNIEFKVCETPIFI
                     PSDFKTKVLAAGEEIIDLICNPAFKQTSKKAIPENCFVPGEEGNPAFIALDFGICVDD
                     AGELIPQLIEMQGFASLYAWQHELGKQYKAHFPIPDTVDHLFNGLDESSYIQLLKKII
                     IGNHQAENVIILEIEPDKQKTWIDFYLTRSMLNIEPVCISKIIKEGKDLFYLKNGVKT
                     KIHRIYDRLIFDELQARKDLLLQWNLTDEVNVEWVCHPNWFFRISKYTLPFIKSKYVP
                     ETIFLNELKSIPDDLENWVLKPLYSFAGHGVKFNVTKQDIEEVMPHGDVFILQKKVTY
                     KPVVATLDEPAKAELRLMYLWDEGESRPQLVINLARLSKGVMIGVDYNKNKTWVGGTV
                     GYYEK"
     gene            complement(1724..2542)
                     /locus_tag="PLB46_12245"
     CDS             complement(1724..2542)
                     /locus_tag="PLB46_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HRF77338.1"
                     /translation="MPEAKKLKLAILDMYENAPNEGMRCIKAIAQAHADFVDWKVFDV
                     RHKNEVPGNEYDIYISSGGPGSPFDGEGKEWESKFFTLLDKLWAHNANGHSKKKYVFG
                     ICHSFQLMSRHFELGNVCKRKSTAFGVFPIQKTEVAKHDRFFRNLPDPYYVVDSRDWQ
                     MIEPDLDKLAALKANVLAVEKRRDHVPLPRAVMAMSLGEHFYMTQFHPEADAEGMLRL
                     FAKPEKRDHIVQNHGDWKLDEMIRNLSDPEKLPLTHKELIPSFLRSSINALRMS"
     gene            2716..>3844
                     /locus_tag="PLB46_12250"
     CDS             2716..>3844
                     /locus_tag="PLB46_12250"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF77339.1"
                     /translation="MKPNQSAYLAVIFFICFVINGFVAKSQVAPGELPNIVFIMADDG
                     RYDEYRVTGGPDWFIAPNIERIANEGANFSRTYAPTPICGPSRASIYTGLYAHEHKTA
                     NNGDTLNTALPKIQEILHDQGYYTGFIGKYGNGFQPPFEFDYWVDIGDNEIYKNFWIK
                     VNGENVFVPGHITNSFNDYINEFFDSVTVHSDQPFALFFFPLAPHTPNTPRATDASLY
                     YDAIPPFPENFYPYDSLYPEFYNESGSTWVKDTIATKDFIKDRFACLIGLDENINKIF
                     NRLDTTHVTDSTFILYTSDNGYINGEHMMRAKALPIDESIHVPLFIRYPEWFSDSTII
                     DDDIIELIDIPKTLLEIAGVEDTFNFSGLSIRELAEPDTMRH"
BASE COUNT         1165 a          727 c          656 g         1296 t
ORIGIN      
        1 gttttcattt gcttcttcaa cctggaattt ttttaaaaac tgtttgtcag tagtatattt
       61 actatgttgt tggataaaaa acgatacaag tttttgttca ctgctttttg gcgtaattaa
      121 aatatgtccg taatcaattg ttgtttgtaa ataaccaaat aaatgttccg tatgaccaaa
      181 ataaccaacc tcgggattgt cgggtgaagc tgccagtaaa acacaatata attgtagatt
      241 gtctaattgt aaaataaaat caggttcata aatgaaggat tgtggaacaa gggtagcatg
      301 ttcaaaatta tccataaata aaccgtcagt atcaagaaaa tgttgttccg ttaatacatg
      361 cttacaattt aaaacaaaag ctcgtttttt agcttttgct gcttcaacta atgctaattg
      421 ggcctgatat tttttatcat tcatgaggga aatataattg gcgacttcca attgaattat
      481 ttttcatagt agcccactgt tccgccgacc catgttttat ttttattgta atcaacacca
      541 atcattacac ctttacttaa tcgcgcgaga ttaattacta attgtggacg cgattcacct
      601 tcgtcccaca aatacataag tctgagttct gctttagcgg gttcatctaa tgttgctaca
      661 accggtttgt aggtaacctt cttttgtaat ataaaaacat cgccatgcgg catcacttct
      721 tcaatatcct gtttagtaac attaaatttt acgccatgcc cggcaaatga gtataatggt
      781 tttaataccc aattttccaa atcatcaggg atggatttta attcatttaa aaatatcgtt
      841 tcaggaacat atttgctttt aataaaaggt aaggtgtatt tacttattct gaaaaaccag
      901 ttggggtgac aaacccattc aacattcact tcatcggtta aattccattg caataataaa
      961 tcttttctcg cctgtaactc atcaaaaatt aatctgtcgt aaatacgatg gattttcgtt
     1021 ttcacaccat tttttaggta aaacaaatct ttgccttctt taatgatttt tgaaatacaa
     1081 acaggctcaa tatttaacat cgagcgagtt aaataaaaat caatccacgt tttttgtttg
     1141 tccggttcaa tttctaaaat gattacattt tccgcttgat gattgcctat aattattttt
     1201 ttcaatagtt gtatataact gctttcgtct aaaccattaa ataaatgatc taccgtatcg
     1261 ggaataggga agtgggcttt gtattgttta cctaattcat gttgccatgc atacaaagat
     1321 gcaaatccct gcatttctat taattgcgga attaattcgc ccgcatcgtc aacacaaata
     1381 ccaaaatcca aagcaataaa cgcaggatta ccttcttctc ccggaacaaa acaattttcc
     1441 ggaatcgctt ttttactggt ctgtttgaat gcaggattac atattaaatc gattatttct
     1501 tctcctgcag ccaatacttt tgttttaaaa tcggaaggaa taaaaatagg cgtttcgcat
     1561 actttaaatt caatgttttt atcgtatttg tcctgacaag catgtacaaa tttttgataa
     1621 tcagcttctg tgaaagcctt attatattgg tcgcgaattg attttatcat gaattacaaa
     1681 cttagcaata aaaatagcag aatttgagga ataggcaagt gcattaactc attcttaatg
     1741 cattaatgga agacctcaaa aatgagggta tcaattcctt atgtgttaat ggcagttttt
     1801 cagggtcact gaggttacga atcatttcat ctaacttcca atccccatgg ttttgaacga
     1861 tatggtcgcg tttttccggt ttggcaaaca aacgcaacat accttcagca tcggcttccg
     1921 ggtgaaactg cgtcatataa aaatgttcgc ctaaactcat tgccattaca gctctgggta
     1981 acggcacatg gtcgcgtcgt ttttctacag ctaaaacatt ggctttgagt gcagcaagtt
     2041 tatccaaatc tggctcaatc atttgccagt cgcgactatc cacaacgtaa taaggatcag
     2101 gcaaattcct aaaaaatcgg tcgtgtttag caacttccgt tttttgaatg ggaaatacac
     2161 caaatgcagt tgatttccgc ttacaaacat tacctaattc aaaatgtcgg ctcatcagtt
     2221 gaaaggaatg gcaaatgcca aatacatatt tctttttgct gtgaccgttt gcattatgtg
     2281 cccaaagttt gtctaaaagt gtaaaaaact tactttccca ttcttttcct tcaccatcaa
     2341 aaggactgcc ggggccgccg gaggaaatat aaatgtcata ttcattacca ggaacttcat
     2401 ttttatgtct gacatcgaat actttccagt cgacaaaatc ggcatgagcc tgcgcaatag
     2461 ctttaatgca acgcatccct tcattgggtg cattctcata catatcgaga atagcgagct
     2521 ttaacttttt tgcttcaggc ataacgcgag gcgaagttat ttatttttaa tagcttaatt
     2581 ggtgatgttt tccaccacct taacatttac cacttcctgt gttaagaaca cattagttta
     2641 gataaaggtt ttcttttaaa tttgaaacgt gattgtccta aaaatattcg tgaaacaagc
     2701 aaagcaaatt ttggaatgaa accaaaccaa tctgcctacc ttgcagtaat cttctttatt
     2761 tgctttgtta taaacggttt tgttgcaaaa agtcaggttg cccctggtga attgcccaat
     2821 atcgttttta ttatggcgga cgatggcagg tacgacgaat accgcgttac cggcggaccg
     2881 gactggttta ttgctcccaa tatagagcga attgccaacg aaggggctaa tttttcacgc
     2941 acttatgcac caactcctat ttgtggaccc agccgggctt ccatttatac aggtttgtat
     3001 gcccacgaac ataagacagc gaacaatggt gatacgctta ataccgcatt gccgaaaatt
     3061 caggaaattt tgcacgatca gggttattat accggattta tcggaaaata tggcaatgga
     3121 tttcaacctc cttttgaatt cgactattgg gtagatattg gtgacaatga gatttataaa
     3181 aacttttgga ttaaagtaaa tggggaaaat gtttttgttc cgggacatat taccaattcg
     3241 tttaatgact atatcaatga attttttgat tcggttacag tgcatagcga ccaacctttt
     3301 gccctgtttt ttttcccatt agcaccacat acaccaaata cgcctcgcgc aacagacgcc
     3361 agtttatatt atgatgcaat tccacctttc cctgaaaatt tttatcccta cgattcgctt
     3421 tatcctgaat tttataatga atcgggctca acttgggtaa aagatactat tgctaccaaa
     3481 gattttatca aggatagatt tgcatgttta atcggattag atgaaaatat caataaaata
     3541 tttaatcgtt tagatactac tcatgttacc gacagcacat ttattttgta caccagtgat
     3601 aatggttata tcaatggcga acacatgatg cgtgcaaagg cattgcctat tgatgaatct
     3661 atacatgttc cgctttttat tcgttatccc gaatggttta gtgattctac tattattgat
     3721 gatgatatta ttgaactgat agatattccg aaaacattat tagaaattgc cggcgttgag
     3781 gatacattta atttttccgg attatctatt cgtgaacttg ccgaaccgga tactatgcgg
     3841 catt
//
LOCUS       DAORKW010000349         3842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1228_length_3842_cov_6.111698, whole genome shotgun sequence.
ACCESSION   DAORKW010000349 DAORKW010000000
VERSION     DAORKW010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1228_length_3842_cov_6.111698"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLB46_12255"
     CDS             <1..578
                     /locus_tag="PLB46_12255"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRF77340.1"
                     /translation="FDEKLEEQLEHYFKLLKFSQNTELPIVNDYATPATLGKDRLAAA
                     VGSSAAFPGKNVLSIDAGTCIKYDFVRADNRYLGGGIAPGLVMRLKAMHNFTARLPLI
                     SLTPQMLVQQTLLTGDSTTDSMLSGAINGALWEVEGAIAAYNAIYDDLHVIITGGDAS
                     FFELHVKSRIFARPNLVLEGLNTILKYNFDN"
     gene            584..2005
                     /locus_tag="PLB46_12260"
     CDS             584..2005
                     /locus_tag="PLB46_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77341.1"
                     /translation="MRRIPLFILFFSITMLSAQVNSPYSRYGLGNIFPTTFGASNGLG
                     GMSAAYFTPNNINYTNPASYADISYTTFDVGAYGNVLTLENDLESYTSGDGNLSYMAF
                     GFPMLKKLRHSKFGLSFGLIPYSAFEYNIIQEEPTDDPVLGMKEYNYVGDGKLYQIYG
                     GLGYKYQSDTVHTGKILNNEPVTATNIVSIGANAAQLFGSLYNITYASFPEQVNSQTT
                     KLTRSNNINGGIYNMGIGLTRQYTRTSDVSRDFLVWRIGGSMSPQINVNGEQSVLWTN
                     IFKTGNYESVTDTIYMEPDTTGNITLPASFQGGLAFSFFTTEQKDKNQFTLSAQYNRT
                     NWSDYKGFQDAGQLGDSWRVTVGGELFTKAKATNTKVSEDKNQRVFNTAPWTIRFAVY
                     SGQSNLIVDGVQLNDRGISSGFSVPLGVGKEVQSTLRNSRLNFFVNAGQRGSNTTITE
                     TYYNFGVGFTIVDMGWFQDYKLN"
     gene            2037..2618
                     /gene="lptC"
                     /locus_tag="PLB46_12265"
     CDS             2037..2618
                     /gene="lptC"
                     /locus_tag="PLB46_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HRF77342.1"
                     /translation="MFRLKTYQQTYRSNYLFSSVLFFTCLLVLSSCVNDMDEVNKTSK
                     LAEPGVERGKNIELFYSEDGRVKVKVTAPAVTRVLAAPPYTEFTEGLKVDFYNDSLRV
                     TSWLTANYGIRYESDGKTVLKNDVQVLNENNEHLSTEELIWDERKHIIYTEKFVKITT
                     PDQVIYGEGMEADEQLTTYRIKKPQGTIKSNIE"
     gene            complement(2619..3137)
                     /locus_tag="PLB46_12270"
     CDS             complement(2619..3137)
                     /locus_tag="PLB46_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRF77343.1"
                     /translation="MPLILPVNDKAPQFGDDCYVADNATIVGEVIMGNQCSVWFNAVV
                     RGDVNSIIIGNKVNIQDNAVIHCTYQQAATTIGNNVSIGHNAIVHGCTIHDNVLVGMG
                     AIVMDHAVVHSNVLIAAGSVVLENSVLESGFIYGGVPAKKLKPLSEEVFKQNIDRIAN
                     NYVMYSSWFKKV"
     gene            complement(3183..>3842)
                     /locus_tag="PLB46_12275"
     CDS             complement(3183..>3842)
                     /locus_tag="PLB46_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRF77344.1"
                     /translation="GCSAGAMVGALYAAGFAPREIFKLIEHRSLYSIIKMGLPNKGMM
                     ELSYFKKILQENIEHDSFELLNLPFYVSVTNLNTGDYEIISNGKLVDYVIASQSIPLV
                     FKPQKIGEHYYVDGGVLNNLPVEPLRDQCDILVGVNVNPINYTDKLTGMRDVGFRVLN
                     LTLKMNMEQRINMCDFVIEPETSEYTIFDINKSKTIYDAGYEAALPVVEKIVSQLKEN
                     Y"
BASE COUNT         1246 a          688 c          729 g         1179 t
ORIGIN      
        1 atttcgatga aaaactggaa gaacaactcg aacattactt caaactgtta aaattttcac
       61 aaaatacgga actgccaatt gttaatgact atgccacgcc tgcaacatta ggcaaggaca
      121 ggcttgcagc tgcagttggc tccagtgctg cgtttccggg aaaaaatgtg ttgagcattg
      181 atgcgggaac atgtataaaa tacgacttcg taagggccga taatcgttat ttagggggag
      241 gtattgcgcc gggactggtt atgcgtttaa aggccatgca caattttacc gccagattgc
      301 cgttaatatc gctcacacca caaatgctcg tgcaacaaac attacttaca ggcgattcta
      361 ccaccgactc catgctttcg ggggcaatta atggtgcact atgggaagtg gaaggtgcaa
      421 ttgctgccta caatgccatt tacgacgatt tacatgttat tattaccggc ggagatgcat
      481 cattctttga attgcatgtg aaaagccgga tatttgcacg cccaaatctg gttttagagg
      541 gtttgaatac tattcttaaa tataattttg ataactgatg gttttgcgac gaattcccct
      601 gtttatttta tttttttcaa taacgatgtt atcggcacag gtgaactcac cttattccag
      661 atatggatta ggtaatattt ttcctaccac ttttggtgcg tcaaatggcc tcggaggaat
      721 gtcggcagct tattttacac ccaacaatat caactatacc aacccggctt cttatgccga
      781 tattagttat accacttttg atgttggtgc atacggcaat gtattaacgc tggaaaacga
      841 tttggaaagt tatacctccg gtgatggtaa tctttcttat atggcatttg gatttcctat
      901 gttaaaaaaa ttgcgtcact cgaaattcgg attaagtttt ggtttaattc cttacagtgc
      961 atttgaatac aatattattc aggaagaacc aacggatgat ccggttttag gtatgaaaga
     1021 atacaattac gttggtgatg gaaaattata ccagatatat ggtggtttag gatataaata
     1081 tcagtctgat acagttcaca caggaaaaat actcaacaat gaaccggtaa ctgcaactaa
     1141 tattgtaagt attggtgcaa atgcagctca attgttcggt tcattatata atattacgta
     1201 tgcttcgttt ccggaacagg ttaattcaca aacaacaaaa ttgaccagaa gtaataatat
     1261 taatggcggt atttacaaca tggggattgg acttacccga caatacacac gcacaagtga
     1321 tgtgtcgcgc gattttttag tttggcgaat tggtggttcc atgtcaccac aaattaatgt
     1381 taatggtgag caatctgttt tatggaccaa catttttaaa acaggtaatt atgaatcggt
     1441 taccgatacc atttatatgg aaccggatac tacaggaaat attacattac ccgcttcgtt
     1501 tcagggtggt ttggctttca gcttttttac aacagaacaa aaagataaaa atcagttcac
     1561 tttgagcgct caatataaca gaactaactg gagtgattat aaaggatttc aggatgcagg
     1621 acaattaggc gacagctgga gagtaactgt tggtggcgaa ttatttacta aggcaaaagc
     1681 taccaataca aaggtaagtg aagataaaaa tcagcgtgta tttaataccg ctccatggac
     1741 aattcgtttt gccgtatata gcggacaaag taatttaatt gttgatggtg ttcagttaaa
     1801 tgatagagga atttcaagtg gtttttcagt gccgttgggt gttggaaaag aagtacaatc
     1861 tacgttaaga aattcacgac tcaacttttt tgtgaatgca ggtcaaagag gaagtaatac
     1921 tactataact gaaacatatt ataacttcgg agttggattt actattgtag atatgggctg
     1981 gttccaggat tataaattaa attaatgatt actaccggaa tgttttttgc aacatgatgt
     2041 ttcgcttaaa aacatatcag caaacgtata ggtcgaacta tttatttagc tccgttttat
     2101 tttttacatg cttgttagtt ttaagcagtt gtgtaaacga catggatgaa gttaataaaa
     2161 cttctaaact cgcagaacct ggagtagaac gcggaaaaaa tatagaatta ttttatagtg
     2221 aagatggtcg ggtaaaagta aaagtaactg cacctgctgt tacccgagtg ctggcagcgc
     2281 cgccatacac tgagtttacc gaaggattaa aagttgattt ttataatgac agtctgcgcg
     2341 ttaccagttg gctgaccgct aactacggca ttcgatatga gtcggatgga aaaacggttt
     2401 taaaaaatga tgtgcaggtt ttaaatgaaa ataacgaaca tcttagcaca gaagaattaa
     2461 tttgggatga acgtaaacac attatttaca ccgaaaaatt tgtaaaaatt actacacctg
     2521 atcaggtaat ttacggtgaa ggaatggaag cagacgaaca actcaccacc tatagaatta
     2581 aaaaaccaca aggcacgata aaaagtaata tagaataatt acactttttt aaaccagctg
     2641 gaatacatca cataattatt agctattcgg tctatatttt gtttaaagac ttcttcgctc
     2701 aagggtttta atttttttgc gggaacaccg ccataaataa atccactttc caatacagaa
     2761 ttttctaata ctacactgcc ggcagcaatt aatacattgg aatgcacaac agcatggtcc
     2821 attacaattg cacccatacc caccaataca ttatcgtgaa tcgtacaacc atgcacaatt
     2881 gcattatggc cgatagaaac attattgcca attgttgttg cagcctgctg ataagtgcaa
     2941 tgaattaccg cattgtcctg aatatttact ttattgccaa ttattatcga attaacatct
     3001 cctcttacca ctgcattaaa ccaaacactg cattgattgc ccataattac ttcacctaca
     3061 atagttgcat tgtcggctac ataacaatcg tcgccgaatt gaggtgcttt atcgttaaca
     3121 ggaagtatta aaggcatagt gatttatttt cagcaaatat aaataaaaca caataggaaa
     3181 gattagtaat tttctttcag ctgactgaca attttttcta caacaggtag tgcggcttcg
     3241 taaccggcat cataaatagt ttttgattta ttgatatcaa aaatagtgta ctccgaagtt
     3301 tccggttcaa ttacaaaatc acacatatta atgcgttgtt ccatattcat ttttaaggtt
     3361 agatttaata cacgaaatcc aacatctcgc atgccggtta gtttatcggt ataattaatt
     3421 gggttaacat taacaccaac taaaatatca cattggtcgc gcaaaggttc aacaggaaga
     3481 ttgttcagta caccaccatc aacataataa tgttcaccaa ttttttgcgg tttaaatact
     3541 aaagggatgg actgtgaggc aataacataa tcaaccagtt tgccattgct gattatttcg
     3601 taatcgcccg tatttaaatt ggtaacggaa acataaaatg gaagatttaa tagttcaaaa
     3661 ctatcgtgtt caatattttc ctgtagtatt tttttgaaat aagacaactc catcattccc
     3721 ttgttgggaa gtcccatttt aataatactg tataaactgc gatgttcaat cagtttaaaa
     3781 atttcgcgag gcgcaaaccc tgcagcatat aaagcaccta ccatggcgcc tgcactgcaa
     3841 cc
//
LOCUS       DAORKW010000350         3834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1231_length_3834_cov_6.669225, whole genome shotgun sequence.
ACCESSION   DAORKW010000350 DAORKW010000000
VERSION     DAORKW010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1231_length_3834_cov_6.669225"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="PLB46_12280"
     CDS             <1..966
                     /locus_tag="PLB46_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRF77345.1"
                     /translation="GAVALARMKRNLVVSWWTNIRFEKSFTRDLCLLLKASGCIGVSG
                     GLEVASDRLLELIKKDVTVEQVARVNKNFTDAGIMVHAYLMYGFPTQTVQETIDSMEM
                     VRQLFENGVLQSGFWHQFAMTAHSPVGLHPEQFHVTIKNNQIGAFANNDLEHLDPTGA
                     DHSKFGEGLKISLFNYMEGAGLDKPVHTWFKEKFPATTIPPNYIESILNNSAPLIANE
                     NHKVIFLGNAPQTNFFTKTKKGVTHQMAALLFSTRTGELEIKLTREHGIWLSAMLQHM
                     KANNETIFTLTQIKENFTSSGLTHFELFWNNPPVNTLFEVGLLHI"
     gene            complement(963..1502)
                     /locus_tag="PLB46_12285"
     CDS             complement(963..1502)
                     /locus_tag="PLB46_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77346.1"
                     /translation="MKRAIIFLIVVLPFLTQAQNDTQPLPCGIIPNTFLDLNSDGIND
                     LNLTGFAVSSDINDPSLAGYCSIGLRAIQNTFLIYQTSAEGIQQLLLLTPEQLENKDV
                     LKQFINNTQLTFETNAYAGFTDWSYGSAVVNTPKKDMLEVSTMVKTVTGEKTLWYIVT
                     FIVEPEKGITLKHQELLDI"
     gene            complement(1568..3157)
                     /gene="rho"
                     /locus_tag="PLB46_12290"
     CDS             complement(1568..3157)
                     /gene="rho"
                     /locus_tag="PLB46_12290"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRF77347.1"
                     /translation="MYTEAQLNDMLVPELREIAESLSINNFKKMGKSELVSAIVGTTK
                     KAVSTHKTSDDKKNDRKRLLRPRKKKELAGDDTDDKVVTPEIAFVEEDDEDVKIEVPE
                     TRDESFNNQSNNQQNNEQPIVEQQTNEQPVVNNREEAPQQQHQYPKKTREPRFNIDLD
                     GAVPGEGVLEMMPDGYGFLRSADYNYLTSPDDIYVSPSQIKLFGLKTGDWVSGFVRPP
                     KEGEKYFALLRVETINGRDPKEIRDRVPFDYLTPLFPEKKLNLVYSPTDYSTRVIDLF
                     TPIGKGQRGMIVAQPKVGKTFLLKSIANAIAQNHPEVYLIVLLIDERPEEVTDMERSV
                     NAEVIASTFDEPAEKHVKVSSIVLQKAKRLVECGQDVVILLDSITRLARAHNTVAPSS
                     GKVLSGGVEANAMQKPKQFFGAARNIEFGGSLTILATALIDTGSKMDEVIFEEFKGTG
                     NMELQLERKLFNKRIFPAVDVTASSTRRDDLLHEREALQRMWVLRNHLADMNTEEAMN
                     FILQHMRGTKSNEEFLATMNG"
     gene            3534..>3834
                     /locus_tag="PLB46_12295"
     CDS             3534..>3834
                     /locus_tag="PLB46_12295"
                     /inference="COORDINATES: protein motif:HMM:NF014458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRF77348.1"
                     /translation="MLDINYLRANTEIAKQRLTKRNKYDIALIDQLLQLDDERKKTQT
                     ALDNTLSETNATSKEIGKMMAQGQKDAAEAMKNKVAELKVQSANLEQQHATIAENI"
BASE COUNT         1195 a          742 c          674 g         1223 t
ORIGIN      
        1 ggtgcagtag ctttagcaag aatgaaacgc aatttagtag taagctggtg gaccaatatt
       61 cgtttcgaaa aaagttttac ccgcgatttg tgtttattgc tgaaagcaag tggatgtatt
      121 ggtgtaagtg gtggcctgga agtggcttct gacagattac tcgaattaat taaaaaagac
      181 gtaactgttg aacaggtagc gcgtgtaaat aaaaatttta ccgatgccgg aattatggtg
      241 cacgcttatt taatgtatgg cttcccaacg caaaccgtgc aggaaacaat cgattccatg
      301 gaaatggtgc gccagctttt tgaaaatggt gttttacaat ccggattttg gcatcaattt
      361 gccatgaccg cccacagccc tgttggttta catcctgaac aatttcatgt taccattaaa
      421 aataatcaaa taggagcttt tgccaataat gatttggagc acctcgaccc aaccggagcc
      481 gaccatagta aatttggtga aggcctgaaa atttccttat ttaattatat ggaaggcgcg
      541 ggattagaca aacctgttca tacctggttt aaagaaaaat ttccggctac aaccattcct
      601 ccaaattata ttgaatcaat tttaaataat agcgcgccat taattgctaa cgaaaatcat
      661 aaagtaattt ttttaggaaa tgcgccgcaa acaaattttt ttaccaaaac taaaaaaggt
      721 gttacacatc aaatggcggc tttacttttt tcaaccagaa caggtgagtt ggaaattaaa
      781 ttaacgcgtg aacatggcat ttggttatca gctatgttac aacacatgaa agcaaataac
      841 gaaaccatat ttactttaac gcaaataaag gaaaatttta catcatccgg tttaacccat
      901 tttgaattat tctggaataa ccctccggta aatacacttt ttgaagtagg tttattacat
      961 atttaaatat ccaataattc ctggtgtttt aatgttattc ctttttcagg ttcaacaata
     1021 aatgttacaa tataccataa agtcttttca ccggtaactg ttttaaccat ggtgcttact
     1081 tctaacatat cttttttagg tgtattaacc actgctgacc catacgacca gtcggtgaaa
     1141 cccgcgtaag cgttagtttc aaaggttaat tgtgtgttgt taatgaattg ctttaaaacg
     1201 tctttattct cgagctgctc tggtgttaat agcagcaact gttgtatacc ttctgccgaa
     1261 gtttgataaa tcaaaaatgt attttgaatt gctcttaatc caattgaaca ataacctgcc
     1321 aaagacggat cattaatatc cgaactaact gcaaatccgg ttaaattcaa atcgttgatg
     1381 ccatcgctgt tcaggtctaa aaaagtattg ggaattattc cgcatggcaa cggttgagta
     1441 tcgttttgag cctgggttaa aaatggtaac acaacaatta aaaatatgat tgctcttttc
     1501 atattcatga tttgtaaatg acataaaaaa aatccgccgt agcggatttt ataaaaataa
     1561 taagtaatta tccattcatg gttgccaaaa actcttcgtt gcttttggtg ccacgcatgt
     1621 gttgtaaaat aaaattcatt gcctcttcag tattcatgtc agcaagatga ttacgcaata
     1681 cccacattct ttgtaatgct tcgcgctcgt gcaacaaatc gtcgcgacgg gttgaacttg
     1741 ctgttacatc tactgccggg aaaatacgtt tgttgaataa ttttctttcg agttgtaatt
     1801 ccatattacc ggttccttta aactcttcaa aaattacctc atccatttta cttccggtat
     1861 caattaatgc tgttgctaaa atggtaagcg aaccaccgaa ttcaatattt cgtgccgcac
     1921 caaaaaattg ttttggtttt tgcattgcat ttgcttccac accacccgat aatacttttc
     1981 cgcttgaagg agcaacggta ttatgtgctc gtgctaaacg cgtaattgaa tcgagtaaaa
     2041 taactacatc ctgtccgcac tcaaccaaac gttttgcttt ttgtaaaaca atactgctca
     2101 ccttcacgtg tttttcggca ggttcgtcga atgtactggc tattacttct gcatttacac
     2161 tgcgctccat atcggtaacc tcttcagggc gctcatcaat aagtaatact attaaataaa
     2221 cttccgggtg attttgtgca atggcattgg caatcgattt caataaaaat gttttaccaa
     2281 cttttggttg cgctacaatc attccgcgtt gcccttttcc aattggtgta aataaatcaa
     2341 ttacacgagt tgaataatct gtcgggctat aaactaagtt taatttcttt tctggaaata
     2401 acggtgttag gtaatcgaag ggtactctgt cgcgaatttc ttttgggtcg cgtccgttga
     2461 tggtttctac gcgtaataaa gcaaaatatt tttcgccttc tttcggaggg cggacaaatc
     2521 ccgaaaccca gtctccggtt tttaatccaa ataattttat ttgcgatggt gatacataaa
     2581 tatcatcagg cgaggttaaa taattataat cggcactgcg taaaaaacca tatccgtcag
     2641 gcatcatttc taatacacct tctcccggaa cagctccgtc taaatcaata ttaaatcttg
     2701 gctctctggt ttttttaggg tattgatgtt gttgctgcgg cgcctcttct ctattattta
     2761 caacaggctg ctcatttgtt tgttgctcga caatcggttg ctcgttattt tgctgattgt
     2821 tggattgatt gttaaacgat tcatcacgtg tttcaggcac ttcaattttt acatcttcat
     2881 catcttcttc aacaaaagca atttcgggag ttaccacttt atcatcagtg tcatcaccgg
     2941 ctaattcttt cttttttcgc ggacgtaata atcgcttgcg atcattcttt ttgtcgtcgc
     3001 tggttttatg ggtactcaca gcttttttgg tcgtaccaac aatggctgac accaattcac
     3061 ttttacccat ttttttaaag ttgttgatcg ataatgactc cgcaatctct ctcagttcag
     3121 gaacgagcat atcgttcaac tgtgcttcag tatacattac aaacttttat aagttaaaaa
     3181 ataaaatttc actaaataca atgtaggttc aatctaaaaa cgaaaataaa ttcaggaaaa
     3241 aaaactgact gaataaaaca aaagaaatat tcagatgaga tgtcctttag ttggttaatc
     3301 ggactttaca gtgcaatgat aaggcggaaa gttgaaataa acaaactcaa aggtaactgg
     3361 tcatctaatt aattgattat gaataagata taaggcaaat aaatttccac tccttacttt
     3421 acatggtatt ataacgccat ttttaaacca aataatatgg caaacaacaa aaacctttgt
     3481 ttattcttta gctacagcgg gcttccacgt atctttgcca aaatttttac ccgatgcttg
     3541 atattaacta cctgagagcc aataccgaaa ttgccaaaca acgattaact aaacgcaata
     3601 aatatgatat tgctttaatt gaccagcttt tgcaattgga cgacgagcgc aaaaaaaccc
     3661 aaaccgcact cgacaatacg ctttcagaaa caaatgcaac aagcaaagaa ataggtaaaa
     3721 tgatggcgca gggtcaaaaa gatgctgctg aggccatgaa aaataaagta gcagaactta
     3781 aggtgcaatc tgccaacctg gaacaacagc acgcaacaat tgcagaaaat atta
//
LOCUS       DAORKW010000351         3832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1234_length_3832_cov_3.064337, whole genome shotgun sequence.
ACCESSION   DAORKW010000351 DAORKW010000000
VERSION     DAORKW010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_3832_cov_3.064337"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="PLB46_12300"
     CDS             <1..1713
                     /locus_tag="PLB46_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77349.1"
                     /translation="DNEGWYNYGAGNTWELGYPAGPVINTPPIATPGSQYSWATSLTG
                     NYNDDELSYLESPCFDISSLLMPFVEVDLWWDTEDTWDGLQLEYTLDGISWTQLGDIG
                     TGENWYTNDAAALGYEFGWVGSGPGWVTAHHDLSFLAGATYVKFRFKFAADGIYNFDG
                     IGLDNFNIQDPFDNDLGVIALLSPTSGPELTAAEPVTINVRNFGLNPQSGFAVNYTVD
                     GGAVVTEIFTGTIDGAEILPYTFATTADLSAIGPHEICAWTSLVGDEDPTNDDIPGCK
                     MVYNFPPVSGTGAYYIYSSAVGFEPGYLNSNSTAMDAVFGPDAWILDYYESLDVLSVF
                     NEDNCFIFLEGSESHWSEFEAFFNNNQDIIEGWVASGGNLFINASPWEGDGGNVGFGD
                     VTISYPYYTYQTAAVDAAHPVLNTPYTPITADYYGFYVSYGRILGDINPILSDYYCAD
                     CYILGDKEWGDGRVMFGTMSAPEYYSPSTESQNLRQNIIDYLKLCSPVDIGVTALLSP
                     DGGCGMTDEEEITIEISNFGPTTVTNTPVKYTIDGGAEVAEIAPGPIPIGGTYNYTFS
                     TTAD"
     gene            1735..2517
                     /locus_tag="PLB46_12305"
     CDS             1735..2517
                     /locus_tag="PLB46_12305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF77350.1"
                     /translation="MATWTDFSGDEDETNDLLTTEVSSLATPVLELGANKTVCDITTL
                     DAGNIGSTYLWSTGATTQTIDVIESGTYSVTVTNPTSGCSVTDNITVTVNYTPSAEFT
                     YTATGLTVTFTNESTAGATYNWSFGDGGSSTLENPSHTYAAEGTYTVTVTVSNGCGSD
                     FYSLVIEVSLSVEDIALAAAIDVYPNPTADVALVTFNLTEYNDIRLELVNTLGQTIWT
                     ALPGNILNGKVTIDMTTLAGGVYQLNVYTNNATATKQIVLTK"
     gene            2789..>3832
                     /locus_tag="PLB46_12310"
     CDS             2789..>3832
                     /locus_tag="PLB46_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77351.1"
                     /translation="MTKHLLKLLSRAAIILAFISAREVSAQDFNYYIYSNVYGGSEPW
                     YTVENSNAMNTVFGAGEWTLDYFETCDPDLVFSANTNFVFLEGSDFCADELENFLITN
                     LPIIENWVESGGRLLLNAAPNEGDGMSFGFGDVYLSYWGGASNVVATDPGHPIFNTPY
                     TPTGTSWTGSSFSHASVSGTGLTPLIVDVSIPSTVAYSEGRYGSDGIVGFGGMTTTNW
                     HTPYTESVNLLQNIIYYLAYYVIPDHDIAVTSVSSPTSSCGLGLETITVTYKNLGLNP
                     ETNIPVSYQLDGGTVVTEIYAGPVAAGESFTVSFGTPGDFSALITHTLVASCDLATDE
                     DPANNSTTISITNI"
BASE COUNT         1122 a          724 c          779 g         1207 t
ORIGIN      
        1 gataatgaag gctggtataa ctatggtgca ggaaatacct gggagcttgg ataccctgct
       61 ggtccggtta ttaatacacc gccaattgca acacctggta gtcagtattc atgggctact
      121 tccttaaccg gaaattataa tgatgatgaa ttatcctacc ttgaaagtcc ttgctttgat
      181 attagttcgt tattgatgcc ttttgttgaa gttgatttgt ggtgggatac tgaagatact
      241 tgggacggac ttcaactgga atatacttta gatggaattt catggacaca acttggcgat
      301 attggaaccg gcgaaaactg gtataccaac gacgcagctg ctttgggata tgaatttggt
      361 tgggttggtt caggccctgg ttgggttaca gctcaccacg atttatcatt ccttgccggt
      421 gctacctatg taaaattccg tttcaaattt gctgcggatg gtatctacaa ctttgatggt
      481 atcggtttag ataacttcaa tattcaggat cctttcgata atgacttagg tgttatagct
      541 ttattaagcc ctacctctgg ccctgaattg actgctgctg aacccgtaac tataaatgtg
      601 cgcaattttg gattaaatcc gcaaagtgga tttgccgtga actataccgt tgatggcggt
      661 gctgttgtta ctgaaatttt tacaggaact attgatggtg cggaaatatt accttataca
      721 tttgcaacaa ccgcagactt aagtgcaata ggacctcacg aaatctgtgc atggacaagt
      781 ttagttggtg atgaagatcc aactaatgat gatattccag gttgtaaaat ggtttataat
      841 ttccctccgg tttccggaac aggagcttat tacatatata gcagcgctgt aggttttgag
      901 cctggttatt tgaactctaa ctccaccgca atggatgctg tttttggccc tgatgcttgg
      961 attttagatt attatgaatc attagacgta ttaagtgtgt ttaatgaaga taattgtttc
     1021 attttcttag aaggaagtga atcacattgg tcagaatttg aagcattctt taataacaat
     1081 caggatatca tagaaggatg ggttgcttca ggtggtaatt tattcataaa tgcttcacct
     1141 tgggaaggag acggtggtaa cgttggattt ggtgatgtaa ctatctccta tccttactac
     1201 acctatcaaa cagccgcagt tgatgctgct cacccggtgt taaatacacc ttatactcct
     1261 ataactgccg actattatgg tttttatgtg agttacggta gaatattagg tgatatcaac
     1321 ccaatattga gcgattacta ttgcgccgat tgttatattt taggagataa agaatggggt
     1381 gatggacgtg taatgtttgg taccatgagt gcaccggaat attattcacc ttcaacagag
     1441 tcacaaaacc tcagacaaaa tattattgat tatctgaaac tttgttcacc tgttgatatt
     1501 ggtgttactg cattattatc accggatggt ggatgtggta tgaccgatga agaagaaatc
     1561 accattgaaa tcagcaactt cggccctaca acagtaacta atactccggt taaatatacg
     1621 atagatggtg gtgcagaggt tgcagaaatt gctcctggtc caattccaat tggaggcact
     1681 tataactaca ctttctctac aacagctgat taagcatctc ctggtgatca tgatgtggcg
     1741 acctggactg atttcagtgg agatgaagat gaaaccaacg acttgctcac aacagaagta
     1801 agttcattgg caacacctgt attggaatta ggtgctaaca aaacagtttg tgatatcact
     1861 acacttgatg caggaaatat cggaagcacc tatttatgga gcactggtgc cactacacaa
     1921 actattgatg ttattgaatc aggcacttat agcgtaaccg ttacaaatcc tacttcagga
     1981 tgttctgtaa cagataatat taccgttacc gttaactata ccccttccgc agaatttacc
     2041 tatactgcaa cgggcttaac agttacattc acaaatgaat caacggcagg tgcaacctac
     2101 aactggagct ttggtgatgg aggaagttca acattagaaa atccatcaca tacatacgct
     2161 gctgaaggca cctatactgt tacagttaca gtttcaaatg gttgtggttc tgacttctac
     2221 agtctcgtaa ttgaagtgtc attaagcgtt gaagatattg cattagcagc cgcgattgat
     2281 gtatatccaa atccgactgc agatgtagca ttagtaactt tcaaccttac tgaatataac
     2341 gatattcgtt tagaactagt aaatacatta ggtcaaacaa tttggaccgc tttaccaggc
     2401 aatattttaa atggcaaagt gaccatagat atgaccacct tagctggcgg tgtatatcaa
     2461 cttaatgtat ataccaacaa tgcaacagca actaaacaaa tagtattaac taaataataa
     2521 aagaagtttt tcactcttta atcgaaaaga ggcttcgcaa gaggcctctt ttctttttta
     2581 agtgatattg ggttatacac atttttgaag ttcatttcgg tttaaccaaa aaacaatgct
     2641 ggcggacatt tcaagccgtt tcgggatgtt atggtggtta aaaatgaaat tattgtatat
     2701 tttttaacac atttattatg aaattaaacc aatggtttcc taatttagac acagaattgg
     2761 tttcttaact aaacactaaa caatttttat gacaaaacac ctacttaaac tcctcagtcg
     2821 tgccgcgata attttggcat ttatctcggc tcgggaagtt tcagcacagg atttcaatta
     2881 ttacatttat tctaatgttt atggtggctc agaaccttgg tacaccgtag aaaactctaa
     2941 tgcgatgaac actgtttttg gcgcaggtga gtggacttta gactatttcg aaacttgtga
     3001 tcctgatttg gtattttcag caaataccaa tttcgttttc cttgaaggaa gcgatttttg
     3061 cgcagatgaa ctcgaaaact tcctcatcac aaacctgcct atcattgaaa attgggttga
     3121 atcaggaggt cgtctcctac tcaacgccgc tccaaatgaa ggcgatggta tgagctttgg
     3181 ctttggcgac gtttaccttt cctattgggg aggcgcaagt aacgttgttg caactgaccc
     3241 cggccatccc atttttaata cgccttatac accaacagga acatcatgga ctgggtcatc
     3301 attttcccat gccagtgtgt ccggaacagg tttaactcca ttaattgtgg atgtatcaat
     3361 tccatctaca gttgcttact ctgaaggccg ctacggttct gacggtattg taggttttgg
     3421 cggtatgaca acaacaaatt ggcatactcc atatactgaa tcagttaatt tattacaaaa
     3481 cattatttat tacttggctt attatgtgat tcctgatcac gatattgccg taacttcagt
     3541 atcaagtcct acctctagct gtggattagg attagaaaca ataacagtaa cctataaaaa
     3601 tttggggtta aacccagaaa caaatatccc ggttagttat caattagatg gcggaactgt
     3661 ggttacagaa atttatgcag gtccggttgc agctggtgaa tcttttacag tatcattcgg
     3721 cactcctggc gatttttcag cactaattac acatactttg gttgcttctt gtgatttagc
     3781 aacggatgaa gatcctgcta ataacagtac aacaatatca atcaccaata tc
//
LOCUS       DAORKW010000352         3822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1240_length_3822_cov_6.629148, whole genome shotgun sequence.
ACCESSION   DAORKW010000352 DAORKW010000000
VERSION     DAORKW010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1240_length_3822_cov_6.629148"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..2663)
                     /locus_tag="PLB46_12315"
     CDS             complement(315..2663)
                     /locus_tag="PLB46_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF77352.1"
                     /translation="MKFLHLILILVVTTTIQAQAIEVEWEKSFGGISYEDAYDIIQTP
                     DSNILIVGEGGPWVAEFSDCGEYGGFIIVKINEDGELLWSKCYGGNEYSLAHAVINCS
                     EGGYAIIGETFAYDGDITGAHGNMDFWVIKVDDAGELEWQLAIGGSSYDSGNDIVQTN
                     EGDYLVVGNSSSINGDINDHHETESTSDLLLAKISSGGELIWTNSYGGIYDDYGESIA
                     KDSLDNIYLTGYSFSASGDVPENKGSTDLWVLKVNTDGGIIWSKSFGGSETDIGNDIA
                     ISNGYIYTVGETYSNDYDVFGSHGGRDGWLIKLTIDGELEYQKCFGGSSFEELFEVEF
                     ISGNEILLTGISGSNNGDLTEHFGLSSKSDFWAITIDTIGNLIWQKSLGGSLGEYAYA
                     GLGINENQFIITGTTYSSDGYVSENGGGSDMWTVKLKVCYDKYFADIDGDGFGDVLTD
                     SVACNTPSGYVSDSTDCIDTDPDIHPFLSDICNSIDDNCNGATDEDAIFITYYLDNDG
                     DFFGDILFDSTSCNVLVGYVENSDDCNDVNAEINPLISETCNGIDDNCNIEVDEGLTI
                     YTFYIDVDGDTYGNPDAQVDTCIETIFGYVNNSLDCNDTIGSIYPGAIELCNYLDDDC
                     DGLTDDNLTYVLSYQDNDGDDFGNPLNDSLSCELPVGYVVNNTDCDDTNAAIYPGAEE
                     LLNGIDDDCDQIADEGLTINDPGINTLTLHPNPTFSTIQINATFDEVGTYTIYSSTGQ
                     LIISGVWNSGNQTISVITLAPGVYSIQLEASNSISSGFFVKL"
     gene            complement(3033..>3822)
                     /locus_tag="PLB46_12320"
     CDS             complement(3033..>3822)
                     /locus_tag="PLB46_12320"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF77353.1"
                     /translation="SIGGTLDDYPYKIIKSTENEVLTCGYTFSSDFDVSPSYESPNYW
                     VVKLKLCDTKYFLDTDFDGFGNALIDSISCETPVGYVLDSTDCNDSIYFINPSVKEIC
                     NYFDDNCNGLIDEGLVYLESYADSDSDNFGNILIDTFSCELPEGYVLDNTDCDDTNAD
                     IYPGAEEFLNGLDDDCDQIADEGLSITDIVKNTISIFPNPVNNILFIQSDVTHQITIV
                     NQLGETILFNNLFIGLNTISVENFPSGVYWVKAENEEMVVWVKE"
BASE COUNT         1258 a          780 c          550 g         1234 t
ORIGIN      
        1 caaggcagat tcaagtcccg cttccggtac ttaaaatagt ctgtctttat gacgggcttt
       61 ttttatgctc gtatatgcta atttttaaat aaaaggtatg cgggcctgtc tgccgcatgt
      121 tgaattttat attattaaaa tattttaatt ataaaagtac atcgtaaata aaatgcacat
      181 cgaaatcaag gcagattcaa gtcccgcttc cggtacttaa aatagtctgt ctttatgacg
      241 gttttttttt attctggtat atttcaaatt tatataggta atgtatgctg agctgcctaa
      301 aataaaattt gagattataa ttttacaaag aatccggagg agattgaatt actggcctct
      361 aattgtattg aatatacgcc tggggcaaga gtaatgactg agatggtctg attaccgctg
      421 ttccaaactc cactaataat taattgtcct gtcgaagaat aaatagtata agttccaacc
      481 tcatcaaaag tcgcatttat ttgtatagta gaaaatgtgg gatttgggtg aagtgtcaaa
      541 gtgttaattc ctgggtcatt aattgttaat ccttcatcgg ctatttggtc acaatcatca
      601 tcaataccgt ttaatagttc ttctgctccg gggtaaatag ctgcgtttgt gtcatcacaa
      661 tcagtattgt ttacaacata accaactggc aattcacacg aaagcgaatc gtttaatgga
      721 ttgccaaaat catcaccatc attgtcttga taggataaaa cataagttaa attatcatcg
      781 gttaatccat cacaatcatc atcgaggtaa ttgcagagtt caattgctcc gggatatata
      841 cttccaattg tgtcgttgca atcaagacta ttatttacat atccgaaaat ggtttcaatg
      901 caagtatcaa cttgggcatc agggttacca taggtgtcac catctacatc tatataaaat
      961 gtataaatag ttaatccttc gtcaacctca atattgcaat tatcatctat accattgcag
     1021 gtttcactta ttaaaggatt gatttctgca tttacatcat tacaatcatc actattttca
     1081 acataaccaa ctaaaacatt acatgacgtg gaatcaaata atatatcacc aaaaaaatct
     1141 ccgtcgttgt ccaagtagta tgtaatgaaa attgcatctt cgtctgttgc tccgttgcag
     1201 ttgtcatcta tgctattaca aatatccgaa aggaaagggt ggatatcggg atctgtgtca
     1261 atacaatcag ttgagtcaga aacataacct gagggtgtgt tacaggcaac agagtccgtt
     1321 aatacatcgc cgaatccatc accatcaata tctgcaaaat atttgtcata acacactttt
     1381 agttttactg tccacatatc tgagccaccc ccgttttcag aaacataacc atcagaggaa
     1441 taggtagtgc cagtaataat aaattggttt tcatttattc ctaaacctgc ataagcatat
     1501 tccccaaggg aacctccaag tgatttttgc catattaaat ttccaattgt atcaatggtg
     1561 atggcccaaa aatcagattt actggaaagt ccaaaatgtt cagttaaatc accattattt
     1621 gatccagata ttccagttag taaaatttca ttaccagaaa taaattcaac ctcaaataat
     1681 tcttcaaaac tgcttccacc aaaacatttt tgatattcta gttcgccatc aattgttaat
     1741 tttataagcc atccatctct accaccatga ctaccaaaaa catcataatc attagaatac
     1801 gtttctccaa ctgtataaat ataaccgttg ctaattgcta tatcatttcc tatatcagtc
     1861 tcagatccac caaacgattt tgaccaaata attcctccat cggtattcac ttttagcacc
     1921 cataagtctg tgcttccttt attttcagga acatccccac ttgcagaaaa tgaataacct
     1981 gttaaataga tattatcaag actatccttg gcaattgatt ctccataatc atcgtaaata
     2041 ccaccataag aatttgtcca tatcaactct cctccactac taattttagc tagcagcaaa
     2101 tcactagtag actcagtttc atggtggtca tttatatcac cattaattga actagaattt
     2161 ccaactacca aataatcacc ctcattcgtc tgaacaatat cattcccact atcgtatgaa
     2221 ctacctccaa tagctaactg ccattctaat tcaccggcat catcaacctt aatcacccaa
     2281 aagtccatat ttccatgagc accagtgata tcaccatcat aagcaaatgt ttctccaata
     2341 atcgcatatc ctccttcaga gcaatttatt actgcatgag ctagtgaata ttcatttcca
     2401 ccgtaacatt tactccataa cagctctcca tcttcgttta ttttcacaat tataaatcca
     2461 ccatattctc cacaatcaga aaactctgct acccaaggtc ctccttctcc aactataaga
     2521 atgttagaat ccggcgtttg aataatgtca taagcatctt catagctgat tcctccaaaa
     2581 ctcttttccc attcaacttc tatagcttgt gcttgtatag ttgtagtgac aactagtatt
     2641 aatattaaat gtaaaaactt cattttcaat cagttgggat ataaagagtt aaaatttcaa
     2701 gcaaaataca aatttttcac aagagcttta tgtaaaaata ttttaattat tcccccaaaa
     2761 aaccaaacca aaaaggtatt cccaaacccg tcccatgagg gagttaactc ccgaataact
     2821 ttcggatgct ggcatccccg aaaaaaatgt tgttttgcca agcatgacaa ttatgagata
     2881 aaagttaatg catgaaaggt gttttttacg tattgtgaca gtatcacaaa attgagtaat
     2941 tggtcgagga tttaagtggc gaattgcatc cgcccataca agcttcggcc atttgcgata
     3001 ggctaagact aatgaaattc agatactgca actcactcct tcacccaaac caccatctcc
     3061 tcattttccg ctttcaccca atacacacca ctcgggaaat tttcaactga tatggtgttt
     3121 aatccaataa ataagttgtt gaataatata gtttctccta attgatttac aattgtaatt
     3181 tgatgtgtta catccgattg aataaataat atattattta ccggattcgg gaaaatactt
     3241 attgtatttt ttacaatatc tgttattgat aaaccttcat cagctatttg gtcacagtca
     3301 tcgtcaagtc cgttcaaaaa ttcttctgca ccaggataaa tatctgcatt tgtatcatca
     3361 caatcggtat tatctaatac ataaccttca ggtaattcac aggaaaaggt gtcaatcaaa
     3421 atattgccaa aattatcgct atcggaatca gcgtaggatt ccaagtaaac taacccttca
     3481 tcaattaaac cattgcagtt atcatcaaag taattgcaaa tttcttttac tgaaggattt
     3541 ataaaatata ttgaatcatt acagtcagtt gaatctaaga catacccaac cggtgtttcg
     3601 caagagattg agtctattaa agcattacca aaaccatcaa aatcagtatc taaaaaatat
     3661 ttggtatcgc aaagtttcaa ttttacaacc cagtaatttg gagactcata gcttggggat
     3721 acatcaaaat cggatgaaaa tgtatagccg caggtcagca cttcattctc tgttgattta
     3781 ataattttgt atgggtaatc atctagtgtc ccacctattg at
//
LOCUS       DAORKW010000353         3807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1253_length_3807_cov_5.747868, whole genome shotgun sequence.
ACCESSION   DAORKW010000353 DAORKW010000000
VERSION     DAORKW010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1253_length_3807_cov_5.747868"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLB46_12325"
     CDS             complement(<1..346)
                     /locus_tag="PLB46_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRF77354.1"
                     /translation="MSTTYQATAAFAANLDATDELKDYRNRFHIPSTNGKSLIYFCGN
                     SLGLQPKSTRAYLEQELTDWQNLGVEGHLHAKNPWLYYHHFLTDATARLVGAKPLEVV
                     VMNSLTVNLNLLM"
     gene            580..2763
                     /locus_tag="PLB46_12330"
     CDS             580..2763
                     /locus_tag="PLB46_12330"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF77355.1"
                     /translation="MQYPDNSNTQFRLAAELVNYTGQHIFLTGKAGSGKTTFLRFIKN
                     NITKNCAVVAPTGVAAINAGGVTMHSFFQLPFEVYLPINAPFTGNLQVTYQHNLFSKI
                     QLNSQKREVMEELELLIIDEVSMLRCDKLDCIDAILRGVRKKNIPFGGVQVLFIGDMY
                     QLPPVAPDAEWQILKEHYNSPFFFDAQVLKNTNVVYVELNKIYRQNEQQFINLLNNVR
                     NNTMTEYDFELLQERYNPLFRPTHDEQYITIASHNFKADKINADELSQLPGEAFTFKG
                     AIEGDFSEKALPAEMQLTLKEGAQIMFIKNDVGEDRKYYNGKIATIKSIHKDTIIVTM
                     RDEGVDFELKKETWENIRYQFNKEKNKIDEEIIGKYIQYPIRLAWAITIHKSQGLTFE
                     KVVIDAGQSFAAGQVYVALSRCTTLSGMVLLSRISAHAIGTDERVAQFALRASQESEL
                     ENLIALEKVRFLQKKLFDVFNWDKPVKLLEAYAQYVPDRSLENKRDAMILTTRLIQAA
                     YEQRDVAEKFKEKLPQLISDYDIQHSPELSIRVTKAVDWFGENMQRSMFIPLVNHLSN
                     INNQARIRKYYNYVVELIGALELHLKNLFEIHYGDKPLYHGVNQYKQFIPDLPAKSTK
                     AKADKAHTAMESLYLYQQGLSVEEIVHARGLTTGTILRHLLSFIETGETTLDNLVSKQ
                     KVEQIKQVVKELETTDLALIRGALGKAYDYTEVKIVLAMISDVSA"
     gene            2910..>3807
                     /locus_tag="PLB46_12335"
     CDS             2910..>3807
                     /locus_tag="PLB46_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HRF77356.1"
                     /translation="MNTIPTVNLADFLSGDQVRKNNFVQSLGKAYQEVGFVAVQNHGI
                     NQQLVDDFYKAVEDFFALPESVKQQYEVPGLAGQRGFTSFGKEHAKNSSAGDLKEFWQ
                     IGQTIEDGEVVSDYYPENVKVSEIPNFNELGYQLYRSFEKSGRSLLQSLALFLGLDEH
                     YFDEKIHNGNSILRAIYYPPITQEPASAIRAEQHEDINLITLLVGASADGLEVMDTAG
                     NWVAVKTAPTEIVVNVGDMLQRLTNNVLCSTTHRVVNPPREKWHTPRFSIPFFLHPKS
                     EMSLNCLPDCITESNPLAYEPIT"
BASE COUNT         1279 a          648 c          755 g         1125 t
ORIGIN      
        1 tcattaaaag attaagattt accgttaatg aattcattac cacaacttct aatggttttg
       61 cgccgactaa ccgagcggta gcatccgtta aaaaatggtg gtaatacaac cagggatttt
      121 tcgcgtgtaa atgtccttcc actcctaaat tttgccagtc tgtgagttcc tgttccaaat
      181 atgctctggt actttttggt tgcaggccta aagaattgcc acagaaataa atcagtgatt
      241 ttccgttcgt tgatggaata tgaaaacggt tgcgataatc tttcagttcg tcggtggcat
      301 ctaaattagc tgcaaatgct gctgttgcct ggtaagtggt agacatgttg caaagttaat
      361 attcccaatt gtgaattttc gataggttgg ttgcaataac ttgaatttaa gaagatagaa
      421 agcacctgac tgtcccaaaa attttacctg gttttaagcg tttctaagcg agttgaacgg
      481 ctatcaccgc ataaatagtg agctgaattt aaccatcatc tattcccctt tatttttaat
      541 acttttacag cgtttaaccc ttaaaaatat tgatataaca tgcagtatcc agacaactcc
      601 aatacgcaat tccgattagc tgccgaatta gttaattata ccggtcaaca tattttttta
      661 acggggaaag ccggtagtgg aaaaactaca tttttgcggt ttatcaaaaa caacatcaca
      721 aaaaattgcg ctgttgttgc gcctaccgga gttgctgcta ttaatgcagg tggagtaacg
      781 atgcattcgt tttttcaatt accatttgaa gtatatctac caataaacgc tccatttacc
      841 ggaaatttac aagttactta ccagcataat ttgtttagta aaattcaatt aaatagtcag
      901 aagcgcgaag taatggaaga actcgagctt ttaattattg atgaggtaag tatgttgcgc
      961 tgcgataaac tcgattgtat agatgctatt ttacgaggcg taagaaaaaa aaatattcca
     1021 ttcggcgggg tacaggtatt atttattggc gacatgtatc aattaccgcc ggtggcccct
     1081 gatgcggaat ggcaaatatt aaaagaacat tataacagtc ctttcttttt tgatgcgcaa
     1141 gtattaaaaa atacgaatgt tgtatatgtt gaattaaata aaatctaccg tcaaaacgaa
     1201 caacagttta ttaacttgtt aaataatgtt cgcaataata ccatgacgga atatgatttt
     1261 gagttattac aagaaagata caatccatta tttcgtccaa cacatgatga gcaatatatt
     1321 accatcgcat cgcataattt taaagcagat aaaataaatg cggatgagct gtcacaactt
     1381 cccggtgaag cctttacctt taaaggagct atagaaggcg atttttcgga aaaagccttg
     1441 cctgccgaaa tgcaattaac gctaaaagaa ggtgcgcaga ttatgtttat caaaaatgat
     1501 gttggtgaag acagaaaata ttacaatgga aaaattgcca ctattaaaag tattcacaaa
     1561 gacaccataa ttgtaactat gcgcgatgaa ggcgtagatt ttgaattaaa aaaagaaacc
     1621 tgggaaaata ttcgttatca gtttaataaa gaaaaaaata aaattgatga agaaataatt
     1681 ggtaaataca tacaataccc tattcgtctg gcatgggcaa ttaccattca caaaagtcag
     1741 ggcttaacat tcgaaaaagt agtaatagat gcgggacaat cgtttgctgc aggacaggta
     1801 tatgtagcgc tgagccgatg tacaacttta tcaggtatgg tcttgctatc gcgtatatca
     1861 gcacacgcta ttggcacgga cgaacgtgtt gcacagtttg cattacgtgc ttcgcaggaa
     1921 agtgaattgg aaaatttgat tgccttagaa aaggtgaggt tccttcaaaa aaaactattt
     1981 gatgttttta attgggacaa accggtaaaa ttattagaag cgtatgcaca atatgtacct
     2041 gaccgttcgc tggaaaataa acgcgatgcg atgattttaa ctacacgctt aattcaggca
     2101 gcatacgaac aaagagatgt tgctgaaaaa tttaaggaaa aattaccgca attaatcagc
     2161 gattacgata ttcaacattc cccggaatta agtattcgcg taaccaaagc agtagattgg
     2221 tttggagaaa atatgcaaag gagtatgttt attccattgg ttaatcattt atcgaatatt
     2281 aataatcagg cacgcattag gaaatattat aattatgttg tggaacttat tggcgcttta
     2341 gaattacatt taaaaaattt atttgaaata cattacggcg ataaaccttt atatcatgga
     2401 gttaatcaat acaaacagtt tatccctgac ttaccggcta aatcaacaaa agcaaaagct
     2461 gataaagctc atacggcaat ggaatcactg tatttatatc agcaaggttt aagtgtggaa
     2521 gaaattgttc atgccagggg attaacaacc ggtacaatat tgcggcattt attatccttc
     2581 atcgaaacgg gtgaaacaac tttagataat ttggtttcca aacaaaaagt ggagcaaata
     2641 aaacaagttg taaaagaact tgaaacaacc gacctggcat taatacgtgg agcattaggc
     2701 aaagcatatg attatactga agtaaaaatt gttttggcaa tgattagtga tgtatcagcg
     2761 taaatatttt tttcacttta aataaatgct aataccaatc agcagcgtgg ttgagttatt
     2821 ttgaagcttc gcgaagggta agcattgtgg aagatatgta atgcactaaa atttttagta
     2881 tacttgcatt acaaaaaccc gcaccaatta tgaatactat tccaaccgta aacctggctg
     2941 attttttatc aggcgatcag gtccgtaaaa ataatttcgt acaaagtctt ggaaaagcat
     3001 accaggaagt aggatttgtt gctgttcaaa accatggtat taatcagcaa ttagtggatg
     3061 atttttataa agctgtagag gacttttttg ccttacctga gtcggtaaaa caacaatatg
     3121 aggtaccggg cttagcggga caaagaggat tcacttcatt tggaaaggaa catgccaaaa
     3181 acagcagtgc cggcgattta aaggaatttt ggcaaattgg ccaaacgata gaagatggtg
     3241 aggtggtaag tgattactat cctgaaaatg tgaaagtatc tgaaataccg aattttaatg
     3301 aattaggata tcaattatat cgcagcttcg agaaatcggg tcgcagcttg ttgcaatcgc
     3361 ttgctttatt tttaggatta gatgaacatt attttgatga aaaaattcac aatggcaatt
     3421 ctatattgag ggccatttat tatcccccaa ttacgcaaga gccggcttct gccattcgcg
     3481 cggagcaaca tgaagacatc aatctaatta cactgttagt tggggcctcg gctgatggtt
     3541 tggaagtaat ggatactgcc ggcaactggg tagcggtaaa aactgcccct accgaaattg
     3601 tggtgaatgt aggagatatg ctgcaacgat tgaccaataa tgtattgtgt tcaaccacac
     3661 atcgggtggt aaatccgccg cgtgaaaaat ggcacactcc acgtttttct atacctttct
     3721 ttttgcaccc caaatcagaa atgagtttaa attgccttcc ggattgtata accgaaagca
     3781 acccgctagc ttacgaaccg attacag
//
LOCUS       DAORKW010000354         3781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1267_length_3781_cov_3.901771, whole genome shotgun sequence.
ACCESSION   DAORKW010000354 DAORKW010000000
VERSION     DAORKW010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1267_length_3781_cov_3.901771"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..2120)
                     /locus_tag="PLB46_12340"
     CDS             complement(3..2120)
                     /locus_tag="PLB46_12340"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRF77357.1"
                     /translation="MSTILYLIPALGLVGLAYMVYIALWVNKRDAGNEKMQGIAKNIA
                     DGAMAFLKAEYRVLLIYVGITAGLLAWLASTSERSDPMVALAFIIGSVFSGLAGYIGM
                     KIATKANVRTTEAARTSLSKALNVSFAGGSVMGLGVAGLAVFGLGSLFILFYYMFVNG
                     GDVNGPQMEVALEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLFGSYVATVLATMVLGREVLSDDAMGGMAPMVFP
                     MMVAGFGLLASIVGTWFVRIKEGGSVQGALNLGNWLSIAITAVGAYFLAKWILPEQMT
                     MIKTGEIITLNGVYAAIITGLVVGALISYFTEFYTSMGKRPVTAIIEQSSTGHATNII
                     GGIYIGMQSTMLPIIVLAIGIVMSYAFAGIYGVAIAAAGMMATTAMQLAIDAFGPIAD
                     NAGGIAEMSALPKEVRERTDILDAVGNTTAATGKGFAIASAALTSLALFAAFMGTAGL
                     TTIDISKAPVLAGLFVGGMIPFLFSSMAIKAVGNAAKSMVEEVRRQFREIPGIMDGTG
                     KPEYDKCVAISTQAAIKQMILPGALAILTPVLFGFGLKGVFEDASSAEILGGLLAGVT
                     VSGVLLAIFQSNAGGAWDNAKKSFEKGVDIDGTTHYKGSDAHKASVTGDTVGDPFKDT
                     SGPSMNILIKLMSIVSLIIAAYL"
     gene            2390..>3781
                     /locus_tag="PLB46_12345"
     CDS             2390..>3781
                     /locus_tag="PLB46_12345"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRF77358.1"
                     /translation="MAQQKEAWGSRVGLILAMAGNAVGLGNFLRFPVQAIQNGGGAFI
                     IPYIVCFLLMGIPLLWVEWASGRFGGTRGHHSTPFILDSMDKRKFWKYIGVFGIFTNL
                     AVAAYYCYLESWTLSWVWHTVTRSFAGQSQEEVAGFFGNYVGLNSVEPIIFWIICLVL
                     NTWILSKGLSGGVEKVAKIGMPLLILFGVFLAVMAVSLNAGEHGAINGGSVGLNFLWT
                     PDFSTIWSPKVWLAAAGQIFFTLSVGMGSIQCYASYVRKKDDIALNAMSAGWMNEFVE
                     VVLGAAIIIPISVGYLGVDKVIEMTKSGGLGLGFRTLPFLFEQWGTVLAIICGVFWFG
                     LLFFAGITSSLAMGTPWMGFMTDEFGWSRQKGAWSFGAIVLILGLPTVLFFNQGVFDE
                     YDYWAGTVSLVVFALAEIILFAWVFGMKRGMQEINSGGDIQVPKIFGFVIKWITPILL
                     LGVFLGALITPAGN"
BASE COUNT         1078 a          785 c          750 g         1168 t
ORIGIN      
        1 ttttataaat acgcagctat aatcaagctg acaatactca ttaatttaat caaaatgttc
       61 atactgggac ctgaggtatc tttaaatgga tcacctacag tatcgcctgt aacggatgcc
      121 ttgtgggcgt cagagccttt gtaatgggtt gttccgtcta tatcaactcc tttttcaaac
      181 gattttttag cattgtccca tgcaccacct gcattactct gaaaaatcgc taataaaaca
      241 ccacttactg ttacaccggc taataagcca cctaatattt cagctgacga agcatcctca
      301 aaaacgcctt ttaaaccaaa tccgaataaa acaggagtta atatagcaag tgctccgggt
      361 aatatcattt gtttgatagc agcttgggta gatatggcca cacatttatc gtattctggt
      421 ttgccggtac catccataat gccgggtatt tcgcggaact ggcgacgcac ctcttctacc
      481 atactttttg ctgcatttcc aactgcctta atggccattg atgaaaacaa aaacggtatc
      541 attcctccaa caaataaacc tgctaataca ggtgctttgg agatatcgat ggttgttaaa
      601 cctgcagtcc ccataaatgc tgcaaataaa gccaatgagg ttaaagctgc tgaagcaatg
      661 gcaaatcctt taccggttgc tgccgttgta tttcctactg catctaaaat atcagttctt
      721 tcacgaactt ctttaggtaa tgcactcatt tcggctatac cacctgcatt atctgcaatt
      781 ggtccgaaag cgtcaatcgc aagctgcatg gctgttgtag ccatcatacc ggcagcagct
      841 attgctacac cgtaaatacc tgcaaatgcg taggacatta caattccaat tgccaaaaca
      901 ataataggca acatggtaga ttgcatgccg atatatatac caccaataat attagttgcg
      961 tgaccggtac tactttgttc aataatagca gttaccggac gtttgcccat actggtgtaa
     1021 aattctgtga aataactaat taaagcccct acaactaaac ctgttataat agctgcatac
     1081 acaccattta aggtgatgat ttctcccgtt ttaatcatgg tcatttgttc agggagaatc
     1141 cattttgcaa gaaaatacgc accaactgca gtaatggcga ttgaaagcca gttacccaaa
     1201 tttaatgcgc cctgaacact gccaccttct ttaatgcgaa caaaccaggt accaactata
     1261 ctcgctaata agccaaatcc ggcaaccatc ataggaaata ccataggtgc cattcctccc
     1321 attgcgtcat ccgataaaac ttcacggcct aataccatgg tagctaatac ggtagcaaca
     1381 taactaccaa ataaatcggc tcccatacct gcaacatcac caacattatc accaacatta
     1441 tcggcaatag tggccggatt acgcgggtca tcctccggaa ttcctgcttc tactttacca
     1501 actaaatcgg ctccaacatc tgctgctttg gtataaatac caccacctac gcgggcaaaa
     1561 agtgcaatac tttccgcacc taatgaaaat ccggttaata cttcaagtgc aacttccatt
     1621 tgcggaccgt taacatcgcc gccattcaca aacatgtagt aaaacaaaat gaataatgac
     1681 ccaaggccaa ataccgctaa tccggcaaca cctaatccca ttacacttcc tcctgcaaac
     1741 gatacattta acgctttgct caaacttgtt cttgcagctt cagtagtacg cacattggct
     1801 ttagtagcga ttttcatacc aatataaccg gctaaacctg aaaatacaga gccaataata
     1861 aatgctaacg caaccatcgg atctgaacgt tcagatgtgc tggctaacca ggcaagtaat
     1921 cctgccgtaa taccaacata tattaataaa actcgatatt cggctttcaa aaaagccata
     1981 gcaccgtcag caatgttttt tgctatgcct tgcatttttt catttcctgc atcgcgctta
     2041 tttacccata atgcgatgta aaccatgtac gccagaccaa ctagaccaag ggcgggaatt
     2101 aaataaagaa ttgtactcat aaattttact aagctgctta aaaagagggt gcaaagttac
     2161 ctttcgggca cgtgaaaatg aaatatgagt tacatttctt tgttgatttt cttacgtttt
     2221 tgaatcaaat ttttggtttt gtagcatttt atatgttttg ttgaactatt atgctatcaa
     2281 aactccactt tttcgctgat tcaatgcaca ttatggtttt ttaacaagct ccaattttgc
     2341 tgacggataa aaatttgtat tttgcgctcc aattaaacaa tcacatttta tggcacaaca
     2401 aaaagaagca tggggttcaa gggtcgggct aattctggcg atggcaggta atgccgttgg
     2461 cttgggtaac ttccttcgat ttcctgtaca ggcaatccaa aacggtggtg gggcattcat
     2521 aatcccttat atcgtatgtt tcctgttgat gggtattcct ttattatggg tcgagtgggc
     2581 atcaggacgt ttcggtggga caagaggaca tcactccaca ccatttattc ttgatagcat
     2641 ggacaaacgg aagttctgga agtatatcgg tgttttcggt attttcacaa acctggcagt
     2701 tgctgcatat tattgttact tagaatcatg gacattgtca tgggtttggc atacagttac
     2761 acgctctttt gctggacaaa gtcaggagga agtagcagga tttttcggta attatgttgg
     2821 attaaattca gttgagccaa ttattttttg gataatttgt ttggtgttaa atacttggat
     2881 tttatctaaa ggtttaagtg gtggtgtcga aaaagtagct aaaattggga tgccgttgct
     2941 gatacttttt ggtgttttcc tggcagttat ggccgttagt ctgaatgctg gtgaacatgg
     3001 tgccattaat ggaggtagcg taggtttaaa cttcctgtgg acccccgact tttctactat
     3061 ttggagccct aaagtctggc tagccgcagc ggggcagata tttttcaccc tttctgtcgg
     3121 tatgggctct attcagtgtt atgcttctta cgtgcgcaaa aaagatgata ttgccctcaa
     3181 cgcaatgagc gcaggttgga tgaatgaatt tgtagaagtt gtacttggtg ctgcgattat
     3241 tattccaatt tctgttggat atttaggtgt agataaagtg attgaaatga ctaaatcagg
     3301 tggtttagga ttaggtttca gaacactgcc atttttgttt gaacaatggg gaacagtatt
     3361 agccattata tgtggtgttt tctggttcgg attattattt tttgcaggta tcacctcatc
     3421 attggcaatg ggaacaccat ggatgggctt tatgaccgac gaattcggat ggtcacgcca
     3481 aaaaggtgca tggagttttg gtgcaattgt tttaattctc ggattaccta ccgtattgtt
     3541 ttttaatcag ggggtttttg atgaatatga ttattgggca ggtactgttt cgctcgtggt
     3601 atttgccctt gctgaaataa ttttatttgc ctgggtgttc ggaatgaaac gaggtatgca
     3661 ggaaataaat tccggtggtg atattcaggt gcctaaaatt ttcggatttg taatcaaatg
     3721 gataacgcca attttattat taggtgtttt ccttggggca ttaattacac ctgccggaaa
     3781 c
//
LOCUS       DAORKW010000355         3780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1269_length_3780_cov_4.034094, whole genome shotgun sequence.
ACCESSION   DAORKW010000355 DAORKW010000000
VERSION     DAORKW010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1269_length_3780_cov_4.034094"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2845)
                     /locus_tag="PLB46_12350"
     CDS             complement(<1..2845)
                     /locus_tag="PLB46_12350"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF77359.1"
                     /translation="MQRTIRILFILFISCASTAAWSQTGSIAGVVIDGENAETLIGAT
                     VIVENVPGKGASTDIDGRYRIEGLTEGTYSIKISYISFKTKTIPGVTVTKGAVTNLDI
                     ALETESQNVFGDSMVVEIKARPRQENLNTVLAFQQKNIAPAEVISRDIISRSPDKNTG
                     EVLKRMSGTTITDGKFAVIRGLSDRYNIALVNGNVLPSTEPDRKSFAFDLFPSAMLDN
                     LIVIKAAQPNLPGDFAGGIIMLNTRDIPDENFVSISIGGGYNAVSTFKEYYTYTGGTK
                     DWLGIDDGIRQLPNAFPANRDLFVDLSVEEKANLGKSFAPWGAYSEASTPIDQSYQVS
                     AGYVHKVGAIGEFGVIGAITYNNSYATNFIERNDYDNEITPIYEYTDDQYKNNVLWGG
                     LLNLSYKINDNNKFSFKNSYSVNSSDITTMRGGLNYSKAVEVQNEYYEFSSNQLTSSI
                     VGGDHFLSGSKTKIKWSAGINTLKRDQPNYRTVNYFKNITPSFEGDTMFQMSPSPFAS
                     PDNLGMFYSYMDEKTYTGGVDVTIPFSIGDAKQALGIGGSYLAKDRTFNARNMGIAAS
                     NDIYFNPDYYEIVVLPVGELLSPAYFADSLFYIDEITNPSDFYAATQTNKAVYAMLDN
                     KIGNKLRVVWGARAEFFNQTISSFEFGASTTPTPVEVDTKETDSVGLKFDLLPSVNIT
                     YAASEKTNIRVSAYKTVARPELRELAPFGYYDIETNSSITGNPSLLATNIYNGDVKVE
                     HFFGAGQVISAGVFYKKFIDPIGQRFYFGTVRELKPINDSLATVYGAEFELRKNLGFI
                     AREVKFLERFTFNTNLALINSNTLLHVADTAIAGAAERQLQGQSDYVVNFGLTYIEPM
                     KGMSFTVLFNQIGRRISEYGNAQYDDIYENPRPLLDGQISIPFNKGNGTVKMNFSDML
                     SKYAIFYQDLDEDGAYDETIDNTIAEGQRIVYVR"
     gene            complement(2986..>3780)
                     /locus_tag="PLB46_12355"
     CDS             complement(2986..>3780)
                     /locus_tag="PLB46_12355"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRF77360.1"
                     /translation="LAISSVTNCSAKLSWVAAAGAASYEVRYRITGGVYSSPVNVGAV
                     TNYTFTGLSATTSYDFQVRSVCAGGAEKSAYVKKTTTTSACGAPTSMTASGITSNSAT
                     ISWVNSCDAVSYNLQYRKVPQKAWTTVVVVPTSKTITGLLANTTYEYKVSTNCIGGGV
                     SPYLPLQTFTTLLRTGNEVIVDAASLEVNPNPAADNVSITIAAQATVNVTITNMVGQM
                     VYNMQNVQVNGAETIDLSLEGYESGLYLVNIQGNNINVTKELVVSK"
BASE COUNT         1191 a          720 c          646 g         1223 t
ORIGIN      
        1 ttcttacata aacaattctt tgtccctcag cgatagtatt atcaattgtt tcatcatatg
       61 ctccgtcctc atctaaatcc tgatagaaga tggcatattt gctcagcata tcgctgaagt
      121 tcatttttac ggtaccatta cctttattaa atggaataga aatctgacca tctaataatg
      181 ggcgagggtt ttcgtagata tcatcatatt gcgcattacc atattcagaa attctgcgac
      241 caatctgatt aaataaaaca gtaaacgaca tgcctttcat tggttcgata tacgtcaaac
      301 cgaaattcac aacataatcg ctttgacctt gtaactggcg ttcagcagca cctgcaatgg
      361 cagtgtcagc aacgtgtaat agggtatttg aattaattaa cgcgaggtta gtattaaatg
      421 taaaacgttc caggaatttc acttcacgtg caataaatcc taaatttttt cgcaattcaa
      481 attcagcacc ataaacggta gctaatgaat cgttgatagg ttttaattca cgaacagttc
      541 caaaataaaa acgttgtccg attgggtcaa taaacttttt gtagaataca ccagcagaaa
      601 ttacttgacc tgcaccaaaa aaatgttcta ctttaacatc accattataa atatttgttg
      661 ctaataaact tggattaccg gtaatgcttg agtttgtttc gatatcataa taaccaaatg
      721 gtgctaattc tcttaattca ggacgagcaa ctgttttata tgctgaaaca cgaatatttg
      781 ttttttcaga agcagcatac gtaatattta cagatggtaa taaatcaaat tttaatccta
      841 cgctgtctgt ttctttggta tctacttcta caggagtagg agtagtgctt gcaccaaact
      901 cgaatgaact aattgtttga ttaaaaaatt ctgctcttgc tccccaaact actcttaatt
      961 tattgccaat tttattatct aacatcgcat acactgcttt gtttgtttgt gtagcagcat
     1021 aaaaatcgct tgggtttgta atttcatcga tataaaataa actgtcagca aaataagcag
     1081 ggctcaatag ttcacctaca ggtaatacta ctatttcata ataatcagga ttaaaataaa
     1141 tatcgtttga agcagcaata cccatgtttc tcgcgttgaa cgtacggtct ttcgctaaat
     1201 aacttccgcc tatacctaaa gcctgtttag catcacctat tgaaaatgga atggtaacat
     1261 ctacaccacc ggtatatgtt ttttcatcca tataagagta gaacatacct aagttatccg
     1321 gagaggcaaa tggtgaaggt gacatttgaa acatggtatc accttcaaat gatggggtaa
     1381 tatttttgaa ataatttact gtgcgataat ttggttggtc gcgttttaaa gtattaatac
     1441 ctgcagacca ttttattttt gtttttgaac cgcttaaaaa gtggtcgcca ccaacaatag
     1501 atgaggttaa ttggttagaa ctaaactcat aatattcgtt ttgtacttct actgctttcg
     1561 aataatttaa tccgccacgc atggtggtaa tgtcagaaga atttacggag tatgagtttt
     1621 tgaaactgaa tttattattg tcgtttattt tatatgataa gtttaataaa ccaccccaca
     1681 atacattgtt tttatattga tcatcggtgt attcgtaaat tggtgtgatt tcgttgtcgt
     1741 aatcattgcg ttcaataaag tttgttgcat aagaattgtt gtaagtaatc gcaccaatta
     1801 caccaaattc gccaattgca cctactttgt gtacataacc tgcggatacc tgataacttt
     1861 gatcaatagg tgtagaagcc tcgctgtatg cgccccaagg agcaaacgat ttaccaagat
     1921 tagctttttc ctcaaccgat aaatctacaa ataggtctct gtttgccgga aatgcattag
     1981 gtaattgtct aataccatca tcaataccta accagtcttt tgttccccct gtataagtat
     2041 aatattcttt aaatgtagaa acagcattat aaccaccacc tatagatata ctcacaaaat
     2101 tttcatcagg aatatcacgt gtattcaaca tgataatacc acctgcaaaa tcgccgggta
     2161 aatttggttg tgcagcttta attacaatta aattatccaa catggcagat ggaaataaat
     2221 cgaatgcaaa cgatttacgg tccggttctg tgctaggtaa tacattacca tttaccaaag
     2281 caatattata tctgtcgcta agtccgcgaa taacagcaaa cttgccatca gtaatagtag
     2341 ttccgctcat acgtttcagt acttctccgg tatttttatc aggacttctg ctgataatat
     2401 cacgtgaaat aacctctgcg ggagcaatat ttttttgttg gaaagctaat acggtattga
     2461 gattttcctg acgaggcctt gctttaattt ctaccaccat tgaatcacca aatacatttt
     2521 gcgattctgt ttccagtgcg atatctaaat tcgttactgc gccttttgtt acggtaacac
     2581 ccggaatggt tttagttttg aaagaaatgt agctgatttt aatcgaatag gtaccttcag
     2641 ttaatccttc tattctgtag cgtccgtcta tatcggtgct tgctccctta cccggaacat
     2701 tttccactat aacggttgca ccaattaaag tttcagcatt ttcgccatca attaccacac
     2761 ctgcaattga gcctgtctgg ctccaggcgg cggttgaagc gcatgaaatg aataaaataa
     2821 ataaaattcg gatagtccgt tgcatattgt aagatttaca tgcaaaggtc tcgccggtat
     2881 gttacctaat ctttattgta acattagatt aacttcaaag caatgtaaac aaaaaagccc
     2941 tccccgtgaa ttgggagggc tttaaattaa actgcaatta aagatttatt tacttacaac
     3001 taactctttg gtaacattga tattattacc ttggatatta accaaataca atcctgattc
     3061 ataaccttct aatgaaaggt caatagtttc agcaccgtta acctgaacat tttgcatatt
     3121 gtaaaccatt tgaccaacca tgttagtgat agtaacattt acagttgctt gagcagcaat
     3181 agtaattgat acattatcag cagcagggtt tggatttact tctaaagaag cagcgtcaac
     3241 aataacttca ttacctgtgc gtaataaagt agtaaaggtt tggagtggta aatatggaga
     3301 aacgccacca cctatacaat tggtacttac tttatattca taagtagtat tggcaagtaa
     3361 accggtaata gttttagaag taggaactac tacaacagtt gtccatgctt tttgaggcac
     3421 tttacgatat tgtaagttat agctaacagc atcacaacta tttacccagc taatagtagc
     3481 agagttgctg gtaataccgg aagctgtcat tgaagttgga gcaccacaag ctgaagtagt
     3541 ggttgttttt ttcacataag cgcttttttc tgcaccaccg gcacatactg aacgcacctg
     3601 gaaatcgtaa gaagttgttg cacttaaacc tgtaaatgta taattagtaa cagcacctac
     3661 gttaacaggt gaactgtaaa caccaccggt aatgcggtaa cgaacttcat aagaagctgc
     3721 acctgctgct gcaacccaag ataatttagc actacaattt gttactgaag aaattgctaa
//
LOCUS       DAORKW010000356         3778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1271_length_3778_cov_4.868386, whole genome shotgun sequence.
ACCESSION   DAORKW010000356 DAORKW010000000
VERSION     DAORKW010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1271_length_3778_cov_4.868386"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..2810)
                     /locus_tag="PLB46_12360"
     CDS             complement(159..2810)
                     /locus_tag="PLB46_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF77361.1"
                     /translation="MRIVTLFTLLFITSALSLQAQNGVIFGKLISDKTEDGVAYATVK
                     YGINAGVYTNVNGEFRITDLGAGTYTLEISCIGYEMYKATNLVVSQNSALDLGEIIMV
                     EKTMQIAEVTITEQQKVYDSRYSGTNNVISAKTIQKIQPVGSEELLRMLPGVNISSDM
                     EQSNRPNISIRGSDPRRSNKILLLEDGSPISPAPYLAPGTYYNVPADRLEGIQVIKGS
                     DVLAYGSNTIYGVVNYITKRPPQDPTLTLKLTGGQRDYFTAVGGYGGTWNKTGAEIQT
                     LYKTFGGYMDNSSIKLFNLSGKIFSEFGEKSTFYLKFNYQNEYLNTTWNGNTPFTFNL
                     DPTINPFDADEFTSRRYGMDGVYNYKLTDKARLQTKIYFSDFYRDWWKQTSTVVHAGD
                     VEEYVGADIYATSFSYLDDVTIGPDDYVRVGKVANGYESTTDSRWQYRVYGVQEKYIQ
                     QYGKHTFEAAVKWHKEYYNDVVVNGDSSKWARSGRLTRDDVFHLNAISLYARNNFEFN
                     RFSVIPIIRYETILLNKVNALANATNPDATGVDFNMLENNFDEFTPGINIIYRDIMVL
                     NSSLEIYGGAYKGFSSPTTAIAFNEVTNGEVTPAVDIANLKPEVSFNQEVGVRMTHNK
                     QVFNTQLSIFNTFISNYYSPARSQAFQTLGTVTMNGIEFAINLEPTKLMKSTNHTFGI
                     GIAATYMQSEITGGTFTDFDLFASTVFHTAATKAELIDKINADRSAYTVYSGADIITT
                     EVLTVDDFDSFTKIQTTLGDEGAAGYEVPYVPNVIINAAINYNYKNIGFSISYNYVSE
                     QYTEYLNFENETADGGIGKLDAYGVLDANLEYSLPIKNHLKSATFFVAGKNITNEIYK
                     ASRLNRATGGIFPAGFRQINGGLRLNF"
     gene            complement(3227..>3778)
                     /locus_tag="PLB46_12365"
     CDS             complement(3227..>3778)
                     /locus_tag="PLB46_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77362.1"
                     /translation="KSYNILLAIIMIGIIGCSKNTNEVTTTTDPIVACAENLSSTKAE
                     LVAWKDTIYTKLDEMEQQETKILESLATVKVPADKNIQTNIAKAAFNELKTKRENMRE
                     LYNVVMDLLAFDETAFAEFKAKAEAGSLSQVELDKEIKFYNNHFEFYQSDIINWRSVM
                     EEIEIESIGNQKKLQAIIAESSK"
BASE COUNT         1164 a          683 c          614 g         1317 t
ORIGIN      
        1 attaaaaaac ttgcttaatt aaatattaaa gtgaaaaagt cgcctaataa ttaatcatta
       61 ataattatag cggtaagaat acctgtaata aaaaaaagag gctatccaaa tggacagccc
      121 cgaaatgttt atagtttgaa ttatggaagt ataattactt aaaaattcaa tcgtaatcca
      181 ccatttattt gtctaaagcc tgctgggaaa atacctcctg ttgcacgatt taaacgcgaa
      241 gctttatata tttcattagt gatatttttt ccggcaacaa aaaatgttgc agatttcaag
      301 tgatttttaa tcggcaaact atattccaga ttggcatcta atacgccata agcatctaat
      361 tttcctatac caccatccgc tgtttcattt tcaaagttga gatattccgt atattgttca
      421 ctaacgtagt tgtaactaat cgaaaaacca atgtttttat aattataatt aatagccgca
      481 ttaataatta catttggcac ataaggcact tcatatccag ctgcgccctc atcacctaaa
      541 gtagtttgta ttttggtaaa tgaatcaaaa tcatctacag ttaaaacttc agttgtaatt
      601 atatctgcac cactataaac ggtataagca gatcggtcgg cattaatttt atctatgagt
      661 tcagctttag ttgccgctgt gtgaaataca gtggaagcga ataaatcaaa atcagtaaaa
      721 gtgccacctg taatttcaga ttgcatatat gttgctgcta tcccgattcc gaaagtatgg
      781 ttggtggatt tcattaattt ggtcggttcc aaatttatag cgaattcaat accattcatg
      841 gtaactgtac ctaaagtttg gaatgcctgt gaacgcgctg gtgaataata attcgaaata
      901 aatgtattga atatagataa ctgtgtatta aatacttgtt tgttatgtgt cattcttaca
      961 ccaacttcct gattaaaact cacttcaggt ttgaggttgg caatatcaac agccggtgta
     1021 acctctccat tggtaacttc attaaatgca atagctgtcg ttggagatga aaatcctttg
     1081 taagcaccac cataaatttc cagcgaacta tttaatacca tgatgtcgcg ataaataata
     1141 ttgataccgg gtgtaaattc atcgaaatta ttttccaaca tattgaaatc aacaccggtt
     1201 gcatcagggt tagtggcatt tgccaaagca tttaccttgt tgagtaaaat agtttcatat
     1261 cttataattg gaattaccga aaaacggtta aattcaaaat tatttcttgc gtacaacgaa
     1321 atcgcattta aatgaaaaac atcatcacgt gttaatcgtc cgctgcgtgc ccattttgag
     1381 gaatcgccat ttaccacaac atcattgtaa tattctttat gccatttaac tgcagcttca
     1441 aaagtatgtt taccatattg ttgaatgtat ttttcctgca caccatatac ccgatattgc
     1501 catctgctgt ctgttgtgct ttcataacca ttagccactt taccaacgcg cacataatcg
     1561 tcaggaccaa tggtaacatc atctaaataa ctaaaactcg ttgcgtaaat atctgcgcca
     1621 acatattctt ctacatcacc tgcatgcaca acagttgatg tttgtttcca ccagtcgcgg
     1681 taaaagtcgc tgaaataaat tttagtttgt aatcgggctt tatctgttaa tttataattg
     1741 taaacaccgt ccataccata tctgcgtgat gtaaattcgt cggcgtcaaa tggattaatg
     1801 gtagggtcaa gattaaaggt aaatggggta ttaccattcc aggttgtatt gagatattcg
     1861 ttttggtaat taaatttcaa atagaaggta gatttttctc caaactcact aaatatttta
     1921 ccggagagat taaataattt aatggagctg ttatccatat accctccaaa cgttttgtat
     1981 aaagtctgaa tttcagcacc ggttttattc catgtaccac cataaccacc aactgcagta
     2041 aaataatcgc gttgtccgcc tgttaatttt aaagttaatg ttggatcctg aggtgggcgt
     2101 ttcgtaatat aatttaccac accataaata gtattggaac catatgctaa aacatctgaa
     2161 cctttaatta cctgtatacc ttctaatcta tcggcaggaa cattgtaata agtacccggg
     2221 gctaaataag gggcaggtga tattggagaa ccatcttcca gcaataaaat tttgtttgat
     2281 cttcttgggt cactgcctct gatactgata ttggggcgat tactttgctc catatcgctc
     2341 gagatattta cgcctggtaa catccttaac aattcttcac tgcctacggg ctgaattttc
     2401 tgtatcgttt ttgcagaaat cacgttgtta gtgccactat atcggctatc gtaaaccttt
     2461 tgttgttcgg taatggtcac ttctgcaatt tgcattgttt tttccaccat tattatttct
     2521 cccagatcca aagcgctgtt ttgggaaacc actaaattag tcgctttata catttcatac
     2581 ccaatacaag aaatttcaag tgtataggtt cctgcaccta aatctgtaat acgaaactca
     2641 ccatttacgt tggtataaac ccctgcatta ataccatatt ttactgtggc atatgcaact
     2701 ccatcctcag ttttatctga aatcaatttc ccgaagatta caccattttg agcttgtaag
     2761 cttaacgcag atgtaatgaa aagtaaggtg aaaagtgtta ctattctcat gttatagtat
     2821 tttaaggtcg taaaattaga tatagataaa aaagttctaa aaacatttac aattaattta
     2881 agattgccac aacctttgcc cggtagaggt attcaaaatt atcattgttt ataggttaag
     2941 gtaattgcat ttattgttgt cctgtttgtt atatgcccta agttatcaat ttaacgtggg
     3001 atattcatta gacgctaatt ttagggtgta attaaagggt gaagttaaat tgttgataca
     3061 accagcgctt caatttcacc aaagcaacaa accggctgtt caattgtaaa agttttttaa
     3121 atgcgatttg aagattaaaa aagaggtaaa aatgttattc aacatcctta cctcttgcca
     3181 ttagaaatgt taatttttaa atattttcga gaacaacgcc atgtttttat ttactgcttt
     3241 cagcaattat tgcctgtaat tttttttgat tacctataga ttcaatttct atttcttcca
     3301 ttacacttcg ccaatttata atatcacttt gataaaattc aaaatggtta ttataaaatt
     3361 ttatttcttt gtcgagttct acttgtgaaa gactacctgc ttctgcttta gctttaaatt
     3421 cggcaaatgc ggtttcatca aatgcgagca aatccattac cacattatac agctcgcgca
     3481 tattttcacg ttttgttttt aattcgttaa aggctgcttt agcaatattg gtttgaatgt
     3541 ttttatcggc tgggaccttt acagtggcta gactttcaag aatttttgtt tcttgttgct
     3601 ccatttcatc tagtttagtg taaatggtgt ctttccatgc taccaactct gcttttgttg
     3661 aggataaatt ttcagcgcat gcaacaatag ggtctgttgt agtggtaact tcatttgtat
     3721 ttttgctaca tccaattatt ccaatcatta ttattgcaag caggatgtta taagattt
//
LOCUS       DAORKW010000357         3774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1273_length_3774_cov_4.175854, whole genome shotgun sequence.
ACCESSION   DAORKW010000357 DAORKW010000000
VERSION     DAORKW010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1273_length_3774_cov_4.175854"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PLB46_12370"
     CDS             <1..1202
                     /locus_tag="PLB46_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77363.1"
                     /translation="DRYALSVKLGVGKATNYFDVMYLKGWDNPNAIINVTDTSIARPA
                     SNSVLGFTLHQQMGKHFLLKLTTAASVFTNDINSSEVEATDKGLIKLIDAFNVNTSTQ
                     FHYAVDGSFGYTGNNFGIGAAYKRISPDYKSMGVYFVTNDVEQYTLIPSVNLWKRKIR
                     LGGSVGFEHDNLAANRSFQTERVIGSANVSINPSPVFGITGNYYNYSIGQIQGIKQLN
                     DTIRLAQVNRGITITPRITLGKKQLRHMILFTYDTRNLDDKNVYTATYTEYQTSTQFI
                     NYAITHIDKGYTITLSANNNAIKSEVVNTSYRGGSVGVSKSFYKNTLNVSVNGGYNII
                     AQTIDGGGNMLNYNTNISYRFFKKHLVSLFGYSNIFNSATATIPSYTDYTVRLQYQYS
                     FAKKSIL"
     gene            1199..>3774
                     /locus_tag="PLB46_12375"
     CDS             1199..>3774
                     /locus_tag="PLB46_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77364.1"
                     /translation="MKATNIRNLITCFAFVLSTSVFAQSPLIVNMQVLPPYSPVLADY
                     MAFDGTSVITITNTTATNYDIKLAVHLTGDNGIEAYTNPGFMPSLPITVAPFATRVVY
                     GSELQFYNDVEYTVIGADVQALAINGIVPDGYYTLCVQALDYFSNVELSADEPSGFIP
                     INIQYLDAPFLISPICGDSINKTYPQNIIFNWITPPGAPLNTQYHFKMVEVIPFNRNP
                     YDAMATATTPSLFETTTTLPLLVYGPAQPPLEVDHTYAWQVTATDPSGNLFFQNDGKS
                     EVCSFIYKNGYINYINNDSVFPVINYPESDSNYIVLNGNMQYYYESENTTTASPDKMG
                     GTVNHVLYPFKNEPVSLVLKYMLKINGGNEVIYLPWNTTGVALPDNDAVLDNSTTSAT
                     GDFSLKISRSNFPDFGLVQQGVTVTIPDADAIQHYFNDEGGSGGIVIPDFGFGFGNVP
                     EDGFNYGTIEENNFNFGNVNDGIGYHFNDPNYNFSQKTIGTPEGETGFGKPTIPTLDK
                     PNTTPLSYSDEISGTFTGDLYRVVQVYHNNAYYCMPMESFQPLDTGSTYVGTLETRVR
                     DYILHVDINQIGEGGTVNFINNDPENYITLDGGFDNDFMFFDAEYLYMDDDNDDGIYT
                     TPGEWELDESVMASIAPLYRYSIYRETRSDDAPETEGMNLGNSFDDSDNLYFSEDNLA
                     DYEIGIMGDDDDDNGGMNINGTPVFDYEFQNEIIGLAPKDYGAGRVLISRTESKNGTA
                     SFNKLLKSLSDDDYYILVIEPVDENFINPAIEVRFLYDYDDYSKVDKALYNTQYTTPE
                     VDLDNTENTILSGTAVVEGQLNFKYDDPAITSSFPLKNVTITLQRLQYVIGDDGIKYY
                     ST"
BASE COUNT         1194 a          620 c          729 g         1231 t
ORIGIN      
        1 acgacagata tgcattatcg gttaaactcg gtgtgggtaa agctactaat tattttgatg
       61 tgatgtattt aaaaggctgg gataatccca atgccataat taatgttacc gatacatcta
      121 ttgcgcgacc tgcaagtaat tcggtactgg gatttacttt acatcaacaa atgggtaaac
      181 attttttact caaattaact actgctgcaa gtgtatttac taatgatata aatagcagtg
      241 aagtggaagc aacagataaa ggattaataa aattaataga tgcatttaat gtaaatactt
      301 ccacccaatt tcattatgct gttgatggct cgtttggtta taccggaaat aattttggca
      361 ttggtgctgc ctacaaacgg atatcgccgg attataaatc gatgggtgta tattttgtca
      421 ccaacgatgt ggaacaatac acattaattc catcggttaa tttatggaaa cgcaaaatac
      481 gattaggtgg aagtgttgga tttgaacatg ataatttagc tgccaaccgc tcgtttcaaa
      541 cggaacgtgt aattggttct gccaatgtga gtattaatcc ttctcctgtt tttggcataa
      601 ccggcaatta ttataactac agcatcgggc aaattcaggg tattaaacaa ttaaatgata
      661 ctattcgttt ggcgcaggtt aatcgcggta ttactattac acctagaata acgttaggta
      721 aaaagcagtt gcgccacatg atattattta cgtatgacac gcgcaattta gatgataaaa
      781 atgtgtacac tgctacttat accgaatatc aaacttcaac acaatttatt aattatgcca
      841 ttacgcatat cgataaaggt tataccataa cattaagtgc gaataataat gccattaaaa
      901 gtgaagtggt gaataccagt taccgcggtg gttctgttgg tgtttcaaaa agtttttata
      961 aaaacacttt aaatgtgagc gttaatggcg gttacaatat tattgcacaa accattgatg
     1021 gtggtggtaa tatgttgaat tataatacca atatcagtta tcgttttttc aaaaaacatt
     1081 tggtgagttt attcggctat tctaacattt ttaatagcgc tactgccact attccttctt
     1141 atactgatta tactgtccga ttacaatacc aatattcatt tgccaaaaaa tctattttat
     1201 gaaagcaact aacattcgaa atttaattac ctgtttcgca tttgttttat caacaagtgt
     1261 ttttgcacaa tcgccattga ttgtcaacat gcaggtatta ccaccatact caccggtatt
     1321 ggccgattat atggcatttg acggcacttc ggtaattacg attacgaata ccacagctac
     1381 caattacgat atcaaactgg cggtgcattt aaccggtgat aatggaattg aagcctatac
     1441 caatccgggt tttatgccat cgttaccaat aacggttgcg ccatttgcta cacgtgtggt
     1501 gtatggcagt gaattacaat tttataacga tgttgaatat acggtaattg gtgccgatgt
     1561 gcaggctttg gcaataaatg gtattgttcc agacggatat tataccttat gtgtgcaggc
     1621 attggattat ttttccaatg ttgaactttc ggcagatgaa ccttcaggat ttattccaat
     1681 taatattcaa tatttggatg caccattttt aatttctcct atttgtggtg attcgataaa
     1741 taaaacgtat ccgcaaaata ttattttcaa ttggattacg cctccgggtg cgccattaaa
     1801 tacacaatat cattttaaaa tggtggaagt aattccattc aatagaaatc cttatgatgc
     1861 catggcgact gccactacac cttcattatt tgagactact actacccttc cgttattggt
     1921 atatggtccg gcacaaccac cattggaagt tgaccatact tatgcctggc aggtaacggc
     1981 tacggaccca tcggggaatt tattttttca gaatgatgga aaaagtgaag tatgttcatt
     2041 tatttataaa aacggatata ttaattatat taataatgat tccgtttttc ctgttattaa
     2101 ttatcccgaa agtgattcta attatattgt attaaatggt aatatgcaat attattatga
     2161 aagcgaaaac accaccactg catctccgga taaaatgggt ggaacggtta atcatgtgtt
     2221 gtatcctttc aaaaacgagc cggttagtct tgtattaaaa tacatgttaa aaataaacgg
     2281 tggcaacgaa gttatttatt tgccatggaa cacaacaggt gtggctttgc ctgataatga
     2341 tgcggtttta gataatagta ccaccagtgc aaccggcgat ttttcgctca aaatatcacg
     2401 cagtaatttt cctgattttg gattagtgca acaaggtgtt acggtaacga tacctgatgc
     2461 tgatgccatt caacattatt ttaatgatga aggtggttca ggtggtatag taattcctga
     2521 ctttggattt ggttttggca atgtaccgga agatggcttt aattatggca ccattgagga
     2581 aaataatttt aatttcggga atgtaaatga tggcattgga tatcatttta atgacccgaa
     2641 ttataatttt tcgcaaaaaa caataggcac gccggaaggt gaaacaggtt tcggtaaacc
     2701 tacaattcca actttggaca aaccgaatac aacgcctctc tcctattcag atgaaatatc
     2761 cggcacattt acaggcgatt tatatcgtgt agtgcaggtg tatcacaaca atgcgtatta
     2821 ctgtatgccg atggaaagtt ttcagccttt agataccggt tcaacgtatg ttggcacgtt
     2881 ggaaacacgt gtacgcgact atattttaca cgttgatatt aatcaaattg gtgaaggtgg
     2941 aacagttaat tttatcaata acgaccctga aaattatatc actttagatg gtggttttga
     3001 taatgatttt atgttttttg atgctgagta tttatacatg gacgatgata atgatgatgg
     3061 catttacacc acaccgggtg aatgggaatt ggatgaatcg gtaatggcat ctattgcgcc
     3121 attgtatcga tatagtattt atcgtgaaac acgttcagat gatgcacctg aaactgaagg
     3181 aatgaattta ggcaattctt ttgacgactc ggataactta tattttagcg aagacaattt
     3241 agcggattat gaaattggca ttatgggtga tgatgatgac gacaacggtg gtatgaatat
     3301 caatggcacg cctgtttttg attatgaatt ccaaaacgaa attatcggac ttgcaccaaa
     3361 agattacggt gcaggaaggg tattaatttc acgaacagaa tcgaaaaatg gcacagcatc
     3421 atttaataaa ttgctcaaaa gtttaagcga tgatgattat tatattttgg tgatagaacc
     3481 tgttgatgag aattttatta acccagctat tgaagtgcgt tttttatatg attacgacga
     3541 ttatagcaag gtagataaag ccttatataa tactcaatat actacgccgg aagtagattt
     3601 agacaatacc gaaaatacca ttttaagtgg aacggctgta gttgaaggtc agcttaattt
     3661 taaatatgat gacccggcaa ttacttccag ttttccatta aaaaatgtga ccattacatt
     3721 acaacgtttg caatatgtaa ttggtgatga tgggataaaa tattattcta ctat
//
LOCUS       DAORKW010000358         3773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1274_length_3773_cov_4.694190, whole genome shotgun sequence.
ACCESSION   DAORKW010000358 DAORKW010000000
VERSION     DAORKW010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1274_length_3773_cov_4.694190"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..696
                     /locus_tag="PLB46_12380"
     CDS             250..696
                     /locus_tag="PLB46_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77365.1"
                     /translation="MDTKEKQILVTIVNTILIFAIYAYYVYDRYLIDNPELLNDSSFW
                     GKTFLVLIPVAILAQIVLHIIFAIINGILTRETMDIITDERDKLIELKAIRISHWLFT
                     FGFLLAMGSQAIGMETWVMFVTLVASGFISGIISESAKIYFYRKGV"
     gene            698..946
                     /locus_tag="PLB46_12385"
     CDS             698..946
                     /locus_tag="PLB46_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRF77366.1"
                     /translation="MSNKIKIDKCAITNNIRKLRFMANEMTQQDLADKTGVTRQTIVA
                     IENGKYFPTLELAFRIARVFETPLDQVFEFQFIPSEKS"
     gene            952..1929
                     /locus_tag="PLB46_12390"
     CDS             952..1929
                     /locus_tag="PLB46_12390"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRF77367.1"
                     /translation="MQAVYCTQYGAPEVLELKEVPTPTPKSHEVRIKIYATSVTAADF
                     RIRSFSIPKGYAFFAKLALGFNKPRKPILGTEVSGVIDAVGNKVTKYKVGDAVFALTL
                     GKFGGYAEYVCLNENASMALKPENISFEEAATLPVGALTAQHFIRVAQLTPNHNILIY
                     GASGSVGTFAVQFAKLTGAHITGVSSVSNKEMVAALGVNTWLDYNAPDFEQQLAKYDV
                     VFVAVDKLPFEIANRILKPKGMYINVTSPFKTSAMKKAARLEQKIIVTGKNPAVGIPE
                     MQQLKSVVEQGKIIPVIDKTFSLQQIVEAHHYVDLGHKKGNVSIQVYGG"
     gene            complement(1963..2277)
                     /locus_tag="PLB46_12395"
     CDS             complement(1963..2277)
                     /locus_tag="PLB46_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HRF77368.1"
                     /translation="MSKDFGMPEFMVDVNLHDVNSDEFLKLIPSQRAIVNDLLFEGKI
                     TSYCVSVDRSKLWITMVANDEEDIIEQLAKFPLINFMDCEIAPLLFYNSSYQTFSHIS
                     LN"
     gene            complement(2349..2843)
                     /locus_tag="PLB46_12400"
     CDS             complement(2349..2843)
                     /locus_tag="PLB46_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRF77369.1"
                     /translation="MKTLYIIRHAKSDWAEAGLHDIERPLANRGLRDAPIMAGVLKKR
                     GKIPEIMVTSPALRAFTTCRIFCEQLEYDTANIVIQPELYFGEISLIEKMLQAAFTET
                     DTVAIFGHNPTFSELANHFAPEFGGEMPTCAIVAISFDPKQKATELAGKGKLLWFEYP
                     KKNR"
     gene            complement(2840..>3773)
                     /locus_tag="PLB46_12405"
     CDS             complement(2840..>3773)
                     /locus_tag="PLB46_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77370.1"
                     /translation="GGFFGYHFVVVFFLCFPVSAFAIRPLFQKTDASTNYLDFKKWMI
                     ITFWVVMIVFSIVQTKIAHYSSMSYFPFTFLGAYGLYATVDKWKKWEVAILIFTGTVF
                     ISLALLIPFLLQHPSLIAEYVKDEFSNQALYAESNWNGLEIIPPIMVLIALIVALYYN
                     SRNMVQKAVWVLFISAAVAINLSTVLFVPNIERYSQGAMIDFLKEKNQEDCYKEVIGY
                     KSYAHLFYGQRKPTDNINPTFLTWLKTTHPEEEYTAARYRSLFSFWLVYGNIDKPAYF
                     VTTVRKKDGFDQMQGMELIGERNGYVFFKRLPTK"
BASE COUNT         1258 a          683 c          664 g         1168 t
ORIGIN      
        1 taagtaacaa atgatgacat agcgccccaa cctaagaggc aactgactgg taaattcagc
       61 ctaggctagt agtgctttcc cccattttta aggtttcagg cattaaaaaa gttattgttt
      121 agccttgaaa gtcagtaaaa tcagctattc aaggataaat ttgttaaaat atgtcaaata
      181 tctttgacat tatgtataac atttttacct ttgtgtcatt ataactatac aattactaaa
      241 tacaattata tggacaccaa agagaaacaa atactcgtaa ccatcgttaa caccatcctg
      301 atttttgcga tttacgccta ttatgtatat gaccgctacc taattgacaa tccggaatta
      361 ctcaatgaca gcagtttttg ggggaaaaca tttttagtgc ttataccggt agccatatta
      421 gcacaaattg ttttgcatat catttttgct atcattaatg gaattcttac cagagaaacg
      481 atggatatta tcactgatga acgcgataaa ttaatcgaat taaaagccat cagaatttct
      541 cactggttat ttacattcgg atttttatta gcaatgggct cacaagccat tggcatggaa
      601 acttgggtaa tgtttgtaac actggttgct tccggtttta tttccggcat aatatcagaa
      661 tcagctaaaa tttattttta tagaaaaggt gtataagatg agcaataaaa taaaaatcga
      721 taaatgtgcc atcaccaata atatccgcaa gcttcgtttt atggctaatg aaatgacgca
      781 acaggatctt gctgataaaa caggtgttac aaggcagaca attgttgcca ttgaaaatgg
      841 aaaatatttt cctacactgg aattagcatt tcgtattgca cgtgtatttg aaacaccatt
      901 agatcaggta tttgaatttc aatttattcc atcagaaaaa tcataactat tatgcaggca
      961 gtttattgca cacaatatgg cgctccggaa gtattagaat taaaagaggt cccaacccct
     1021 acacctaaat cacacgaagt acgtataaaa atttatgcca cttcagttac tgcagccgat
     1081 tttagaatca ggagttttag tatcccaaaa ggttatgcat tttttgcaaa acttgcactt
     1141 ggatttaaca aaccaagaaa acctatttta ggcacagaag tatctggtgt tattgatgca
     1201 gtaggtaata aagtaactaa atataaagtg ggtgatgcgg tatttgcatt aacgttaggt
     1261 aagtttggtg gctacgccga atatgtttgt ctgaacgaaa atgcttctat ggcattaaag
     1321 ccagaaaata tttcatttga ggaagctgct actttacctg ttggtgcatt aactgcacaa
     1381 cattttattc gtgtagcaca attaacccct aatcacaaca tattaatata cggtgcatca
     1441 ggtagtgtag gcacttttgc agtacaattt gccaaattaa ccggtgccca tattaccggt
     1501 gtgagcagtg tttcaaataa ggaaatggtt gctgctttag gtgtaaatac ctggttggat
     1561 tataatgcac ctgattttga gcaacaactt gcaaaatacg atgttgtatt tgtcgctgtc
     1621 gataaactgc cgtttgaaat cgccaatcgc atattaaaac ctaaagggat gtatatcaat
     1681 gttacttcgc cgtttaaaac atcggctatg aaaaaagctg caagattaga acaaaaaata
     1741 atagtcaccg gaaaaaatcc tgctgttggt ataccggaaa tgcaacaatt aaaatcggta
     1801 gttgagcaag gtaaaataat acctgttatt gacaaaacat ttagtctgca acaaattgtt
     1861 gaagcacatc actatgtgga cttaggacat aaaaaaggga atgtttccat ccaagtttat
     1921 ggaggataat ttgagactta ttgtaatttt tacaataagt agttaattca acgaaatatg
     1981 agagaaagtc tggtagcttg aattataaaa aagcaatgga gcaatttcgc aatccatgaa
     2041 gttaattagc gggaatttag ccagttgttc gatgatatct tcctcatcgt tggctaccat
     2101 ggtaatccaa agtttagaac ggtccaccga aacacaatag ctggtaattt ttccttcaaa
     2161 caacaggtcg tttacaatag cacgctggct cggaatgagt ttaagaaact catctgagtt
     2221 cacatcgtgt aagttaacat caaccataaa ttctggcatt ccgaagtctt tagacataca
     2281 aatgtagtgt ttttgatgat atagtataaa cgagaataaa acggtttagg ttgtattcga
     2341 tctaaatttt aacgattttt tttggggtat tcaaaccaca acagttttcc tttacctgct
     2401 aattcggttg ctttttgttt ggggtcaaaa ctaatggcaa caatagcgca agtaggcatt
     2461 tcacctccga attcgggagc aaaatggtta gctaattccg aaaaagtagg attatgacca
     2521 aatatggcta cggtatcagt ttctgtaaaa gctgcctgta acattttttc aataagtgaa
     2581 atttccccga aatataattc cggttggata acgatatttg cagtatcgta ctcaagttgc
     2641 tcacaaaaaa tacggcaggt agtaaaagcc ctaagtgccg gactagtcac cattatttcc
     2701 ggaattttac cacgtttttt caatacccct gccattattg gagcatcacg caatcctctg
     2761 ttggccaatg ggcgttcaat gtcgtgtaat ccagcttctg cccaatcgga tttagcatgt
     2821 cgtatgatat ataacgtttt catttagtcg gtagccgttt gaaaaataca taaccatttc
     2881 tctcaccaat taattccata ccctgcattt gatcaaatcc gtcttttttg cggactgtgg
     2941 ttacaaaata agcgggttta tcaatattac cgtaaaccag ccaaaacgaa aataaacttc
     3001 tataacgagc cgctgtgtat tcctcttcag ggtgtgtggt ttttagccat gtcaaaaaag
     3061 taggattaat attatcggta ggttttcgtt gaccataaaa caaatgcgca taacttttat
     3121 atccgattac ttctttataa caatcctcct ggtttttttc tttcaaaaaa tcaatcatag
     3181 ctccttgtga atatctttcg atattaggca caaacaacac tgttgataaa ttaattgcaa
     3241 ccgccgcaga aataaagagt acccatactg ctttttgaac catatttcgg ctattgtaat
     3301 aaagggcaac aataagtgct attaatacca ttatcggagg aattatctct aacccattcc
     3361 aattactttc ggcatataaa gcctggttcg aaaattcatc tttcacatac tccgcaatta
     3421 atgaagggtg ttgcagcaga aaaggtatca acaatgctaa actaataaat actgttcccg
     3481 taaatataag aatggctacc tcccattttt tccatttatc gacagttgca tataatccgt
     3541 atgctcctaa aaaggtaaac ggaaaatagg acattgatga ataatgtgca attttggttt
     3601 gcacaattga aaatacaatc attaccaccc aaaaagttat aatcatccat ttcttaaaat
     3661 ccaggtaatt ggtagatgca tccgtttttt gaaataatgg cctgattgca aacgcagata
     3721 ccggaaaaca taaaaagaat accacgacaa aatgataacc aaaaaaccct cca
//
LOCUS       DAORKW010000359         3765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1278_length_3765_cov_3.946361, whole genome shotgun sequence.
ACCESSION   DAORKW010000359 DAORKW010000000
VERSION     DAORKW010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1278_length_3765_cov_3.946361"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PLB46_12410"
     CDS             complement(<1..604)
                     /locus_tag="PLB46_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77371.1"
                     /translation="MLSKFSLVSFCLLLLAGLKVNGQQYIYNYNIPFTENGRALKYPT
                     AGGLNNPQFSKIDINQDGKQDIFMFDRSGNKPMMLINNGSSGEVNYTYWQDYESIFPK
                     MYDWSILLDYNCDGLEDIITGFENGIKTYLANFDGSNIYFTEDIAKLDFKEAGFTFFL
                     SVGVIDVPGFADINFDGDIDVLTFNMAGGIVDYYENRQIED"
     gene            773..925
                     /locus_tag="PLB46_12415"
     CDS             773..925
                     /locus_tag="PLB46_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77372.1"
                     /translation="MKAKNVQRIVCAEGSQNVGPGRIASKPSPDQIGRQREVITIQKP
                     APAVRN"
     gene            complement(940..3591)
                     /locus_tag="PLB46_12420"
     CDS             complement(940..3591)
                     /locus_tag="PLB46_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRF77373.1"
                     /translation="MFNFGFGQHIKSILQIIAVLAVITPIVSGCNATKYLQDNEYTYK
                     SSEIDFTGPIPADVDSADLADDLVDLSKLEANEKLLGIFPLKTWLFMLGDTAIDQYIK
                     YQEVYDTRFMFVFDYDTLLKKIKPLANPDSRFRNWLTNKAGEQPQFIDSVKIAETEQR
                     IGNYLYNRGYFYATESSSISYDSIKQTGTVTYHVEPGTLYRMKKVYYEIADKGLLFKI
                     NAIPTKSQLETGKPIDVDVLKAERNRLSDNLRNAGYYRFQKEYIYFEVDTASGTDSLD
                     IYVRVSNPVRDTVHHPFEIKNIYVYPNASIDYDPAGIPSFAVHFTDSATVKRISKNKV
                     LKKYMRSDTTVAGKSMISQYNGDQINKKTLLKYYIKNDSNPQKQFIVKQNGQIKRVKR
                     KKLRSDYYLINSPDNFTAKSIANNVFVNPESSYSDSLIQRTVASFSTLGMFKYVTVQA
                     EEMWDSTSYLQFINLIIRLDPLEFKSVSYEFNASTTSDYLLGNSLNLSYTQKNLFHNL
                     DQLKFNIKGGIETQLGGEQTFINTSELNTGLSLSLPRFMWPAPVDVPKRYFPKTDLKV
                     NFSYVNQINDFTLYNTSFEYAVSVFENTKQNKAQKQHILKMPIPTVNIVRVPQISDAF
                     LEELNQNPLLKQSFEEQVIMGLGYTFILNTQPTGFHVFDHYLRASAEVNAPFSDFIRV
                     DADYRSYININKSNRIVSRASFGIATPYWWVKDSTNTFYTEVIPYVKQFFTGGAYSVR
                     AFTVRKIGPGGYVNYDTTDYIRIDQVADLKLEFNFEYRFDIISILEGAVFCDIGNTFT
                     LKNDPFRPHAQFQLNDFYKLMAVGPGLGLRLDFGYFVMRVDAAYPLYDPALDGPYRQE
                     IYDYYNGIGFDIPAKKVAINLAIGYPF"
     gene            3565..>3765
                     /locus_tag="PLB46_12425"
     CDS             3565..>3765
                     /locus_tag="PLB46_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77374.1"
                     /translation="MLTKAEIKHLQSLKLKKYRQNYDEFIIEGEKLTTEALLGNAEML
                     LLIANAGWIKEHKSTIPANIPLH"
BASE COUNT         1230 a          688 c          657 g         1190 t
ORIGIN      
        1 catcttcaat ttgtcggttt tcgtaataat caactatacc accggccata ttaaacgtca
       61 acacatcaat atcaccatcg aaattaatat ctgcaaaacc gggaacatca attacaccta
      121 cggataaaaa aaatgtaaat ccggcttcct taaaatccag ttttgcaata tcttctgtaa
      181 aataaatgtt gctgccatcg aaatttgcca aataggtttt aataccattt tcaaacccgg
      241 taatgatatc ctccaaccca tcacaattgt aatccaataa aatactccaa tcatacattt
      301 ttggaaatat actttcataa tcttgccagt aagtataatt tacttctcca ctgctgccat
      361 tattaatgag catcattggt ttattccccg aacggtcaaa cataaatata tcctgtttcc
      421 catcctgatt aatatcaatt ttagaaaatt gcgggttatt taatccgcct gcagttggat
      481 attttaatgc acgtccattt tctgtaaatg gaatattgta attataaata tattgttgtc
      541 cgtttacctt taaaccggcg agtaataaca aacaaaaact aactaaggag aatttactta
      601 acatatgcca cataattcat cctaaagata caaacgaaaa tggttactgc ggctgaaaac
      661 aggtaaactg actaaaaaat gaaggggagt ttttgaaatt ggcacggtat tgtatttatg
      721 gaatatcaat taatgcaaca tctcattatt aaacaaatta aaaacgcgag ctatgaaagc
      781 gaaaaatgtg caacgtattg tatgcgcaga gggctcgcaa aatgtagggc ccggacgcat
      841 cgcaagtaaa ccttcacctg accaaatagg cagacaaagg gaagtaatta cgattcaaaa
      901 acctgctccg gcagtccgaa attaattcaa aaaaaagttt taaaaaggat aaccaatcgc
      961 aagattgata gccacttttt tggcgggaat atcaaatcct attccattgt aatagtcata
     1021 aatttcctga cgataagggc cgtctaaagc agggtcatat aaagggtagg ccgcatcaac
     1081 ccgcataaca aagtatccaa aatcgaggcg caatccaaga ccgggtccaa ctgccatcaa
     1141 tttataaaag tcgttaagtt gaaattgggc atggggcctg aaggggtcgt tttttaaagt
     1201 aaaggtatta ccgatatcac aaaaaacggc tccttctaat atggaaataa tatcgaaacg
     1261 atactcgaaa ttgaactcca gtttcaaatc ggctacctgg tcaattctga tataatcggt
     1321 tgtgtcgtaa ttcacataac caccgggacc aattttacga accgtaaatg ctcttacact
     1381 gtaagcacca ccggtaaaaa attgttttac ataagggata acttctgtgt aaaatgtgtt
     1441 cgtgctgtcc ttcacccacc aatatggcgt agctatacca aatgatgccc gggaaacaat
     1501 gcggttcgac ttattgatat taatgtagga acgataatcg gcgtctactc gaataaaatc
     1561 agaaaagggt gcatttactt ctgctgaagc tctgagataa tgatcgaaaa catggaaccc
     1621 agttggttgc gtgttcaaaa taaatgtata tcccaacccc ataattacct gctcttcaaa
     1681 actttgtttt aacaatgggt tctgattgag ctcttctaaa aatgcatcgg aaatttgcgg
     1741 cactcgaaca atattaactg ttggaatagg catttttaaa atgtgctgtt tttgtgcttt
     1801 gttttgtttt gtattttcaa atactgaaac agcatattca aaagaggtat tgtataatgt
     1861 aaaatcgtta atttgattta cgtaactgaa attaactttt aaatccgttt taggaaaata
     1921 tcgtttaggt acatcaaccg gtgcaggcca cataaatcgc ggcaacgata acgataatcc
     1981 cgtgtttaat tcagaagtat taataaaggt ttgttcccca cccaattgtg tttcgatacc
     2041 acctttgata ttaaatttca actggtcaag gttatgaaat aaattctttt gagtgtaact
     2101 taaattaagt gagtttccta ataaataatc agatgtggtg ctggcattaa attcgtatga
     2161 aacactttta aattctaaag ggtcaagtcg aataattaaa tttataaatt gaagatagga
     2221 agttgaatcc cacatttctt ctgcctgcac ggtgacgtat ttaaacattc ctaacgtaga
     2281 aaatgaagct accgttcgct gtataagtga gtcggagtag gagctttccg ggttgacaaa
     2341 tacgttattg gcaatagatt tcgcagtgaa gttatcaggt gaatttatta aataataatc
     2401 tgatcgtaat ttttttcgtt ttactctttt tatttgacca ttttgtttga caataaattg
     2461 cttttgcgga ttactgtcgt ttttaatgta atacttcaat aaagtttttt tattgatttg
     2521 gtcaccgtta tattgagata tcatggactt gcctgcaacc gtagtgtcac tccgcatgta
     2581 tttttttaat actttatttt tactaattcg ttttacggtt gcactatctg taaaatgtac
     2641 ggcaaatgat ggtattccgg ccgggtcgta atcaatggaa gcgtttggat aaacatatat
     2701 attttttatt tcaaacggat gatgtacagt atcgcgcacg ggattactca cgcggacata
     2761 aatgtctaat gagtcagttc cggaagcagt atctacttca aaatagatgt attctttttg
     2821 aaaacggtaa tatcctgcat tccgtaaatt atcgctcaac ctattgcgtt ctgcttttaa
     2881 tacatctaca tcaataggtt tacctgtttc taattgactt tttgttggaa tggcatttat
     2941 tttaaataac aaacccttat ccgcaatttc ataatatact tttttcatgc ggtataatgt
     3001 gccgggctcc acatgatagg taacagtacc ggtttgttta atagagtcgt aagaaatgga
     3061 cgatgattcg gtggcataaa aataaccgcg attatacagg taattgccaa tacgttgctc
     3121 tgtttctgca attttaaccg agtcgataaa ttgtggttgt tctcctgctt tattggttag
     3181 ccagtttcta aaacgtgaat caggattagc taaaggttta atttttttca ataaggtatc
     3241 gtaatcaaat acaaacataa aacgcgtgtc atacacctct tgatatttga tatactgatc
     3301 aatggctgta tcccccaaca tgaacaacca cgtttttaac ggaaatatac ccaacagttt
     3361 ttcattcgcc tccagcttac ttaaatcaac caaatcatcc gccaaatctg ctgaatcaac
     3421 gtctgcaggt attggtcctg taaaatcgat ctcactcgat ttataggtat attcattatc
     3481 ttgaagatat ttagtagcat tacaccctga aacaattggt gtaataaccg ctaaaacagc
     3541 aattatctga agtattgatt tgatatgttg accaaagccg aaattaaaca tctacaaagt
     3601 ctgaagctta agaaatatcg ccaaaattat gatgaattta tcatagaagg cgagaaatta
     3661 accacagagg ccttgttggg aaacgcagaa atgctgcttt taattgcaaa tgcggggtgg
     3721 attaaagagc acaagtcaac gatacccgct aatatcccgt tgcat
//
LOCUS       DAORKW010000360         3762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1281_length_3762_cov_5.169679, whole genome shotgun sequence.
ACCESSION   DAORKW010000360 DAORKW010000000
VERSION     DAORKW010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1281_length_3762_cov_5.169679"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..393)
                     /locus_tag="PLB46_12430"
     CDS             complement(46..393)
                     /locus_tag="PLB46_12430"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF77375.1"
                     /translation="METYKLNGTFFYCPVDLTLSVIGGRWQGLIIWNLRDEPKRFNEL
                     KKILVVINDKMLSQTLKTLVEQGVVSRMDYKSTPPKVEYALTETGKALLSIFKQMEIW
                     GKTINNQQLQMAE"
     gene            507..1193
                     /locus_tag="PLB46_12435"
     CDS             507..1193
                     /locus_tag="PLB46_12435"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HRF77376.1"
                     /translation="MKKITSITLMLLLMTTTTKLFGQQPAKLIVSPMPSAQHSAADEA
                     TLATAVAVSQSILEGNWAKLDELLDDNFTYTGDGYVFTKDQYIGFMQDMRAAFSDFEM
                     TLTKSIVDGEFASIQFTSNVVNTGKFMGAPANKKLIRVDGIFMRNIKNGKVMQEWQTT
                     DLLGTMTQIGGGALFFYSVFVGGFGVKTKPPVRKGDDFLYVDGKVVNYDLLPAKEKNK
                     YVKNYMKNSK"
     gene            complement(1460..2635)
                     /locus_tag="PLB46_12440"
     CDS             complement(1460..2635)
                     /locus_tag="PLB46_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77377.1"
                     /translation="MPRFFIAIMVLLYTNNTFAQCEVEISPDGLVNLCEGGYVELTTT
                     VTAVELSVDQQQLLYLGGESARNLPGYSTWQSFTAGVTGDLVQIDQGFFNEMTGTATL
                     NIFTGTGNGGTLLYTETVTISGTGNFWETFVIDPPVSITENEVYTFEVVPDLAGGLPD
                     PYGVQVSGPADNYAGGRCEFDVTWDDVFRTYVSAPISYTWSTGATSSAIIVNEPGTYT
                     VNINAGPGCSATDAVDVVITEVNTNVTVTDFIILTAEATDATYQWINCYDNEIIEDAS
                     SQTYFPDENGEFAVIVTQNGCTDTSECIYIQIESVLTSNFGDFKVVPNPATNNIQFQF
                     DAGINLLSIVTFDIQGNVIQIPFNNNYQANIQTLAPGLYFSKINLPNESFIIKWVKN"
     gene            complement(2846..>3762)
                     /locus_tag="PLB46_12445"
     CDS             complement(2846..>3762)
                     /locus_tag="PLB46_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77378.1"
                     /translation="KLENELSDRHQLIPFTHYAIAFPDSTYEMFKVLNGAFSYKSKNN
                     PVVFQIMPNEDPVFDFLYMYEGKSEFSFNSDKIENSIKQRNKISSGNQVGLYILFQID
                     SLSHVDSISGVTNHTMLLLIGDSVNSLTVITTLTNDEYLKWGNSVSESLKSLIYLPDS
                     MTNSMEDFPFVFEECNKSMQFSKYFFGMMIFTPNSSQDTISKNKIMLSITPTVMDLEY
                     DTSMTKTFVTSRLEFFEYEIVALEQIEFNGYYGYQGYGKSDDCGLGFIFSYTKENQVI
                     NGWGVADEYNQNNIDLFSNFIASTKSNN"
BASE COUNT         1231 a          644 c          660 g         1227 t
ORIGIN      
        1 aacgtaaaaa actatttcgc agaaattaat ttgcatgagt agtatttatt ctgccatttg
       61 caactgctga ttattgattg ttttacccca gatttccatt tgtttaaaaa tggatagtaa
      121 tgctttaccg gtttccgtaa gtgcatattc cacttttggt ggtgttgatt tataatccat
      181 acgacttact acaccttgct ctactaaggt ttttaatgtt tgggataaca ttttgtcgtt
      241 gattactact aatatttttt tcagctcatt aaatcgtttt ggttcatcgc gtaaattcca
      301 gataataagg ccttgccaac gacctccaat aacacttaat gtgaggtcta ccgggcagta
      361 aaaaaatgtg ccgttaagtt tatatgtctc catgccacaa atgtaaatac aatattggca
      421 ctacccaaaa caacagcact tcctttttgg taagtctatt gtgtgcaggg aaaaagacgt
      481 tcacctttgc tgttcaaaat aacgacatga aaaaaataac ttcaattaca ttaatgcttt
      541 tacttatgac aacaacaaca aaattgtttg ggcaacagcc ggcgaaacta atagtaagcc
      601 caatgccatc agcacagcac agcgctgcag atgaagcgac acttgctacg gcagtcgctg
      661 tatcacaatc tatcctcgaa gggaattggg cgaaattgga cgaattatta gacgacaatt
      721 ttacctacac cggcgatggt tatgtattta ctaaggacca atacatcgga tttatgcaag
      781 acatgcgtgc agcattttct gattttgaaa tgaccttaac taaatcgatt gttgatggtg
      841 aatttgcttc tatccaattt acttcaaatg tggtgaatac cggcaaattt atgggagcac
      901 ctgctaataa aaaacttatt cgtgtagatg gcatttttat gcggaacatc aaaaatggca
      961 aggtgatgca agaatggcaa actacagatt tattgggcac catgactcaa ataggtggcg
     1021 gtgcattatt cttttactct gtttttgttg gaggatttgg cgtaaaaact aaaccaccgg
     1081 taagaaaggg tgatgacttt ttatatgtag atggcaaagt tgtgaattat gatttattgc
     1141 ctgcaaaaga gaaaaataaa tatgtgaaaa actatatgaa gaatagtaaa taaattgtat
     1201 ttcagttaat aggtttgggt tatgtgatta atggtgttaa cagccgaaac cacagagcgc
     1261 tcaaagtgaa cacacaaagt ccacaaaagg tactgaacac tttttgtgga ctttattttt
     1321 tatgcaataa tttggtgtaa aatgggtcta gaataaagta aggatacaaa tgaaattgcc
     1381 aatagtgttt tgttgttaaa tcgtaaacaa cttggttgat gtatttacta taataacttc
     1441 acaacagttt atatctagat taatttttca cccatttaat tataaatgat tcattgggca
     1501 agtttatttt actaaaatat aaaccggggg ctaatgtttg aatgttggcc tgataattat
     1561 tattaaaagg aatctgaatt acatttccct gaatatcgaa agtaactatg gataagagat
     1621 ttattcctgc atcaaattgg aattggatat tattagtagc gggatttgga acaactttaa
     1681 aatcaccgaa atttgatgtt aaaacagatt caatttgtat ataaatacat tcagatgtat
     1741 cggtacaacc attttgtgtt acaataacgg caaattcacc attttcatca ggaaaatacg
     1801 tttggctgga tgcatcttca ataatttcat tatcgtaaca atttatccat tgataggtag
     1861 catctgtagc ttctgctgtt aatattataa aatcagttac agtaacattt gtatttactt
     1921 cagtaattac aacatctact gcatcagttg ctgaacatcc ggggcctgcg ttgatattta
     1981 cagtatacgt tccgggctca ttcactataa tagctgaaga agttgcgcca gtgctccaag
     2041 tgtaggaaat aggtgcagac acataagttc taaaaacatc atcccaggta acatcaaatt
     2101 cgcatctgcc acctgcataa ttatctgcag gtccggaaac ctgaacacca tatgggtcgg
     2161 gtaacccacc tgctaaatca ggaacaactt caaaagtgta cacttcattt tctgtaattg
     2221 aaacaggagg atcaataaca aatgtttccc aaaaattacc tgttccggaa atggttactg
     2281 tttcggtata taataaagtg ccaccgtttc ctgttccggt aaaaatattc aaggttgcgg
     2341 tacctgtcat ttcattaaaa aaaccctggt caatctgcac taagtcgcct gttactcctg
     2401 ccgtaaacga ttgccatgtg ctgtagccgg gtaagttccg tgcactttcg ccacctaagt
     2461 ataacaattg ttgctggtcc acagataatt caacagcagt aactgtggtt gtcaattcaa
     2521 catacccgcc ttcacataaa tttaccagcc catcaggtga aatttccact tcgcattgag
     2581 cgaaagtgtt gttggtatat aacaatacca ttattgcaat aaaaaatcta ggcatagggt
     2641 aaaaaaattt aagtgattgt acaatttaca aaaatatcgc aattggttgg gtaaatatgt
     2701 aacagaagtt actggtgtgg gaatgataaa agtcagttgg gtgatttttt attttaggaa
     2761 taaaatgtag aggttaggca aattttctat ggatcgggag acccgggatt ggctgcgcag
     2821 tcggagactg cgcagagctg ggatattagt tattagattt tgttgatgcg ataaaatttg
     2881 agaataggtc tatattattc tggttatact catcagcaac tccccacccg tttatcactt
     2941 gattttcttt tgtataacta aatataaatc ctaatccaca atcatctgac tttccataac
     3001 cttggtatcc atagtaacca ttaaattcta tttgctctaa ggctactatt tcatattcaa
     3061 aaaattccaa tcttgatgtg acgaatgttt tcgtcatact ggtatcgtat tccaaatcca
     3121 taacagttgg tgtaatactt aacataattt tatttttcga tattgtatct tgcgaggaat
     3181 taggagtaaa aatcatcatt ccaaaaaaat attttgaaaa ttgcattgac ttattacatt
     3241 cttcaaaaac aaatggaaaa tcttccatcg aattagtcat ggaatccggt aaataaatta
     3301 atgatttgag tgattcgcta accgaattac cccatttcaa atattcatca tttgtaagtg
     3361 ttgtaattac agttaacgaa ttgacactat ctccaattaa aagtaacatt gtatgatttg
     3421 taaccccaga aatactatcg acatgactta gactgtcaat ttggaataat atgtacaacc
     3481 ctacttggtt accggaagat attttattcc tttgttttat gctattttca attttatcgg
     3541 aattaaaact aaactcactt ttaccctcat acatatataa aaaatcaaaa acagggtctt
     3601 cattaggcat aatttgaaat actactggat tgttcttgct tttatatgag aaagctccgt
     3661 tcaaaacttt aaacatctca tatgtgctat cagggaaagc gatagcataa tgagtaaatg
     3721 gaataagttg atgtctatca gataattcat tttctaattt aa
//
LOCUS       DAORKW010000361         3762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1282_length_3762_cov_4.990289, whole genome shotgun sequence.
ACCESSION   DAORKW010000361 DAORKW010000000
VERSION     DAORKW010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1282_length_3762_cov_4.990289"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..665)
                     /gene="phoU"
                     /locus_tag="PLB46_12450"
     CDS             complement(3..665)
                     /gene="phoU"
                     /locus_tag="PLB46_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRF77379.1"
                     /translation="MSQIDNPVHNLKEELGQMFQLVSTQLTKAKEALTKFDKDLAREV
                     IQIEKRVNGQELQIDRIAENFIALYSPVAIDLRFVLAVLKINNNLERIGDIAEGISKY
                     IIESNGPLDQKLMEESNVIPMYEAAVRMLEETKVAFESEDTLIARKIFKSDDYLDHIN
                     IEAIQNLTEGMKNDPSKITDALYIISIIRKLERIGDQLKNIAEEIIFYIEAKVLKHAK
                     LK"
     gene            complement(694..1449)
                     /gene="pstB"
                     /locus_tag="PLB46_12455"
     CDS             complement(694..1449)
                     /gene="pstB"
                     /locus_tag="PLB46_12455"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRF77380.1"
                     /translation="MSYKLTADHVNVWYGNFHALKDISIRIRPNTVTAFIGPSGCGKS
                     TFLRLINRMNDYIDGFRMDGHILIDEKNIYAKRVRVEELRKEVGMVFQKPNPFPKTIF
                     DNVVYGLTIRGVKDKHTLEEACESSLRQAALWDEVKDNLKKSALALSGGQQQRLCIAR
                     TLAVKPTVILMDEPASALDPISTAKIEELIAELKQHYTIIIVTHNMQQAARISDRTAF
                     FYLGELVEFDTTVNIFTAPKEEKTQSYITGRFG"
     gene            complement(1454..2299)
                     /gene="pstA"
                     /locus_tag="PLB46_12460"
     CDS             complement(1454..2299)
                     /gene="pstA"
                     /locus_tag="PLB46_12460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRF77381.1"
                     /translation="MNNRLKNNLFKYWAITCTLFGIAMLLIFLINIVIDGVGRLDWNF
                     ITDLPSRKPELAGIYTAIMGTVWILGLTAIIAIPIGVAAGIYLEEYGKKSRFANILEI
                     NITNLAGIPSIIYGILGLEVFVRMFGLGNSVIAGALTLSLLILPIIIVATREAIKAVP
                     KTIREASFALGATKWQTISQQILPAAGGGVVTGVILALSRAIGETAPLIVVGALTYVP
                     FVPQSPMDQYSVLPMQIFNWISRPEKEFATNAAAAIIVLLLITFIMNGFAIYLRNRWQ
                     KKVKW"
     gene            complement(2314..3192)
                     /gene="pstC"
                     /locus_tag="PLB46_12465"
     CDS             complement(2314..3192)
                     /gene="pstC"
                     /locus_tag="PLB46_12465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRF77382.1"
                     /translation="MKKRNEKIIEFFLALSGAITVLTTIGILWVLLYESVLFFKEVSV
                     VEFFTDTQWTPLFSDKHFGILPLISGTILTSFIAILVALPIGLSIAVYLNEYAHPKIR
                     QTIKPVLEILAAIPTVVYGFFALTVVTPFLQTLIPSLAGFNALSPGIVMGIMIIPLIS
                     SLSEDALYAVPKSLREASYGLGATQFQTSFKVLVPAASSGIIVSVILAISRAFGETMI
                     VAVAAGLEPRLTLDPTVPIATITTYIVQVSMGDVPQGSLEFKTIFSAGMMLFVFTFIL
                     NTISARVKKKFYHKYE"
     gene            complement(3248..>3762)
                     /locus_tag="PLB46_12470"
     CDS             complement(3248..>3762)
                     /locus_tag="PLB46_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRF77383.1"
                     /translation="WKQVRASFPDEPLHLYGAGTASGTFDYFTEEICGKKGESRGDYT
                     ASENDNTLVTGVAGDKGGLAYFGMAYYENNKEQLKIVPIDNGSGTGVIPTQETVLNKS
                     YAPLSRPLYIYVSEKALARPEVQAFITFFIDNAPELSKEVGYVPCEASIYETQKGLLK
                     ASMEKNAPAK"
BASE COUNT         1194 a          720 c          618 g         1230 t
ORIGIN      
        1 tattatttaa gcttcgcgtg tttcaacact ttcgcttcaa tgtagaaaat tatttcttcc
       61 gcaatatttt ttaattggtc gccaatacgt tccagcttgc ggataatcga aataatatat
      121 aaagcatcgg taatttttga agggtcattt ttcatgcctt ccgttaaatt ttgaatggct
      181 tcaatattaa tatggtctaa ataatcgtcg cttttaaata tttttctggc gataagtgta
      241 tcttccgatt caaatgcaac tttagtttcc tccaacattc tcactgcagc ttcatacatt
      301 ggaattacat tactttcttc catcaatttt tgatcgagcg gcccgtttga ttcgataatg
      361 tatttggata taccttcagc aatatcgcca atacgttcga ggttattgtt aatttttaaa
      421 actgccaaca caaatcgcaa atcaattgca accggtgaat acaatgcaat gaaattttca
      481 gcaatcctgt caatttgtag ctcttgaccg tttactcttt tttctatttg aattacttca
      541 cgagctaaat ctttgtcaaa ttttgtcaat gcttcttttg cttttgttaa ttgcgtagaa
      601 actaactgaa acatttgtcc caattcttct ttgagattgt gaacaggatt atctatttgt
      661 gacatctttt aaaaaatttg aatgaattta aaatcaacca aaacggccgg taatataact
      721 ttgtgttttt tcttctttag gtgctgtaaa tatatttact gtagtatcaa attctactaa
      781 ttcacctaaa taaaagaacg cagttctgtc gctgatacgc gcagcctgct gcatattatg
      841 tgtaacaata attatcgtgt aatgctgttt tagttcagca attagttctt caattttagc
      901 ggttgaaatc gggtccaaag ccgaagccgg ttcatccatt aatattacgg ttggcttaac
      961 agcgagggtg cgcgcaatac acaaacgttg ttgttgacca ccacttaatg ccaatgccga
     1021 ttttttgaga ttatctttta cctcatccca caaagcagct tgacgaagtg agctttcaca
     1081 tgcttcctcc agggtatgtt tgtcttttac accgcgaatg gttaaaccgt aaaccacatt
     1141 atcaaaaatt gtttttggaa atggattagg tttttggaaa accataccta cttctttcct
     1201 caattcttct acgcgaactc ttttggcata tatatttttt tcatcaatta aaatatgccc
     1261 atccattcgg aagccatcga tataatcgtt catgcgatta atcaagcgta aaaatgttga
     1321 cttaccgcaa ccactgggtc caataaaagc agttaccgta ttaggtctga ttcgaataga
     1381 aatatctttt aaagcatgga aattaccata ccaaacattt acatgatctg ctgttaattt
     1441 atagctcata taattaccac ttaacctttt tttgccagcg attacgcaaa taaatcgcga
     1501 agccattcat aataaatgtg attaataata acacaataat tgcagctgcc gcattggttg
     1561 caaattcttt ttcaggtctg cttatccagt taaaaatttg cattggcaat acagaatact
     1621 ggtccattgg tgattgtggc acaaacggca cataggttaa tgcaccaaca acaatcaacg
     1681 gtgcagtttc tccgattgct ctggataaag cgagaattac accggtaaca actcctcccc
     1741 cggcagccgg taaaatttgt tgcgatatgg tttgccattt tgttgcacct aatgcaaaac
     1801 tagcttctcg aattgttttc ggtacggcct taattgcttc gcgggtagca acaataatta
     1861 tgggtaaaat taaaagtgat aaagttaatg cgccggctat tacactattg cctaatccaa
     1921 acatgcgcac aaatacttct aaacctaaaa taccataaat aatagaaggt ataccggcga
     1981 ggttggtaat attaatttcc agaatattgg caaaacgtga ttttttgcca tattcttcaa
     2041 gatatattcc tgcagcaaca ccaattggaa tagcaattat tgcagttaat cccaaaatcc
     2101 aaactgttcc cataatagca gtataaatac cggctaattc aggtttacgt gaaggcaaat
     2161 cggtaataaa attccaatct aatctgccta caccatcaat tacaatatta attaaaaaaa
     2221 taagcagcat agcaattcca aacaaggtgc aggtaattgc ccagtatttg aaaaggttat
     2281 tttttaatct gttattcatg cttgtgcgct tatttattcg tatttatgat aaaatttctt
     2341 ttttactctt gctgaaattg tatttagaat aaacgtaaat acaaataaca tcatacccgc
     2401 agaaaatatg gttttaaatt ccaatgaacc ttgcggtaca tcgcccatcg aaacctgaac
     2461 aatataggta gtaatggtgg caataggcac agttgggtct aaagttaatc gcggttctaa
     2521 accggcagct acggcaacaa tcattgtttc accaaatgca cgtgaaatgg cgaggataac
     2581 tgaaacaata attcctgatg atgcagccgg aactaatact ttaaatgatg tttgaaattg
     2641 tgttgctccc aatccataac ttgcttctcg caacgatttt ggaacagcat ataaggcatc
     2701 ttcacttaat gaagatatta aagggataat cataatgccc atcacgatac ctggcgataa
     2761 tgcgttaaag ccggcgagtg atggaataag tgtttgtaaa aatggtgtga ctacggttaa
     2821 ggcaaaaaag ccataaacta ctgttgggat agcagctaat atttctaaaa caggtttaat
     2881 agtttgcctg atttttggat gtgcatattc atttaaataa acagcaatac ttaaaccgat
     2941 tggcaatgcc actaatatgg caataaatga agttaaaatg gttccactaa taagtggtaa
     3001 aataccaaaa tgtttatccg aaaatagtgg tgtccattgt gtgtcggtaa aaaactcaac
     3061 aaccgatact tctttaaaga acaataccga ttcatacaac aacacccata aaattccgat
     3121 agttgttaaa acagtaatag caccgctaag tgctaaaaaa aattctatta ttttctcgtt
     3181 ccgtttcttc acaatacaaa aaattgttca ggcagttatt tcactgcctg aacaggtata
     3241 taattaatta tttagccggt gcattttttt ccatgctagc ttttaataaa cctttttggg
     3301 tttcataaat gcttgcttca catggcacat aaccaacttc tttacttaat tcaggagcat
     3361 tatcaataaa gaaagtaata aaagcctgca cttcaggacg agctaatgct ttttcactta
     3421 catatatata taatggacgt gaaagtggag catatgattt gttaagaaca gtttcctgtg
     3481 ttggaataac acctgttccg ctgccattgt cgataggtac tattttaagc tgttctttat
     3541 tattttcata ataagccata ccaaaataag ccaaaccacc tttatcgccg gcaacaccgg
     3601 ttactaaagt attgtcattt tcactagctg tataatcacc acgactttca ccttttttac
     3661 cgcaaatttc ttctgtaaaa taatcaaaag ttccactggc agtacctgca ccatataaat
     3721 ggagtggttc gtcagggaat gaagctctta cttgtttcca ag
//
LOCUS       DAORKW010000362         3761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1283_length_3761_cov_5.088775, whole genome shotgun sequence.
ACCESSION   DAORKW010000362 DAORKW010000000
VERSION     DAORKW010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1283_length_3761_cov_5.088775"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..447)
                     /locus_tag="PLB46_12475"
     CDS             complement(19..447)
                     /locus_tag="PLB46_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77384.1"
                     /translation="MVKQFGLPNYVVWLFLGIAFTAIGVISVFQWTYGIATFILTSAV
                     GFAGYWFFFRPAFVALDIMKSGEPAMAIILDVWETGKTVDHNPRIGLLLEIRHRTMVP
                     YEVELKHNVKKEEMRHYQKGRTLQVKVDSKDPKKVAILAA"
     gene            567..1061
                     /locus_tag="PLB46_12480"
     CDS             567..1061
                     /locus_tag="PLB46_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77385.1"
                     /translation="MNKFSITFTFLLCMFGAKNMVGQSQIDTIKVKADVQITVQKLFD
                     AMRNNDSATLHTIFTTNARLLSVASEKGDIKVFETPIADFIDAVGQPKKEVWNEVIWS
                     YSIELNNEIASVWCPYSFFLEQNGHKQLLHCGVNSIELYLSDNGWQITQITDTREETG
                     CREN"
     gene            1119..2153
                     /locus_tag="PLB46_12485"
     CDS             1119..2153
                     /locus_tag="PLB46_12485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRF77386.1"
                     /translation="MNKTIDKLLTKDVIPDFLIRQNIRKLLRQRLIDETKPNQELQQQ
                     HLMRIVDELKKSPIAIETKAANEQHYELPTAFFQLVMGKHMKYSSCYWNASTPDLTAA
                     ENLALEKTCAHAELENGMKILELGCGWGSLTMFMAAKFPDAQITGVSNSATQKQYIDK
                     TCAERGLKNVRIITADMNVFSLDEQFDRVVSVEMFEHMRNYELLMQKIARMLTAEGKL
                     FVHIFTHQLFTYYFDVIDESDWMSKYFFTGGIMPSDDLLLYFNNDLKIQHHYHWDGTH
                     YEKTANCWLENMDKNRNTIMPILSDTYGADQATKWWVYWRIFFMACAELWGYNKGREW
                     FVSHYLFVKA"
     gene            2163..3413
                     /locus_tag="PLB46_12490"
     CDS             2163..3413
                     /locus_tag="PLB46_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF77387.1"
                     /translation="MQIAIIGTGIAGMGCAHRLHKMHDITVYEQDNYIGGHTNTVYVN
                     EGENRIPIDTGFIVFNNENYPHLNALFNTIKAPIKKTSMSFAAHIVHENIEYCGSSLD
                     GLFAQRKNIFSPRYVRFLMQLNAFNKVCLEVLEKSELQSLSIAEYVNSKGWGKDLLNW
                     YILPMSSALWSTPPDTSAQFPALTLVRFFKNHGFLGLNTQFQWYTVDGGSEEYKKRLI
                     APFKDNIQINAGVVGVKKVEGKIEVKTKNGGTKIFDKVIIATHADQALRMLQNPTEHE
                     TQLLSCFSYQQNKATLHTDSALMPSVKKVWSSWNYRTELIDKNLVASCTYWMNSLQDV
                     SKQKNYFVTINDPGTIKKEAIIKEIMYEHPVFTVDAMQAQQHLSKLNKNGQLFFCGSY
                     FRYGFHEDALMSANAVCDLIEMQQ"
     gene            3438..>3761
                     /locus_tag="PLB46_12495"
     CDS             3438..>3761
                     /locus_tag="PLB46_12495"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HRF77388.1"
                     /translation="MDSALYNCLVMHNRLAPKKHRFHYHVFMFWLDIDKLEQTTQSSK
                     LFAYNKRGIYSFWDTDHFKYPAGDARNGLTTRQKLNDYLLSVGITKMPTQVFLTTHVR
                     MLGYVF"
BASE COUNT         1265 a          632 c          727 g         1137 t
ORIGIN      
        1 aggaagtaaa actagggttt aagcagctaa aatggccact tttttagggt ctttggagtc
       61 aacctttact tgcaatgttc tgcctttttg ataatggcgc atctcctctt ttttaacatt
      121 atgcttaagt tccacttcat aaggaaccat ggtccggtga cggatttcta ataataaacc
      181 gattcgcgga ttgtggtcca ctgttttacc ggtttcccaa acatccaaaa ttatagccat
      241 tgccggttct ccggatttca taatatctaa agccacaaat gccggtctga agaaaaacca
      301 atatccggca aatccaacag cactagtcaa aatgaaagtg gcaatcccgt aagtccactg
      361 aaatacagaa attaccccta tcgccgtaaa tgcgatacct agaaataacc aaacaacata
      421 attgggtagc ccgaactgtt taaccataat gctatacctg tgttttaatg gtattaatgt
      481 taattaactt tatatacgta aaattgtacc gaattgttcg caaaatcaat caactttttc
      541 acatttttat ctgaataaag ctgttcatga ataaattttc aatcactttc accttcctgc
      601 tatgcatgtt tggggccaaa aatatggttg gtcaatcgca aatagacact ataaaagtta
      661 aagcggatgt tcaaattacc gtccaaaagt tgtttgacgc catgcggaat aatgatagtg
      721 caacattaca taccattttt acaacaaatg caaggcttct gagcgtagca tccgaaaaag
      781 gagatataaa ggtttttgaa actccaattg cagattttat tgatgccgtt ggccagccga
      841 aaaaagaggt ttggaatgaa gtaatttgga gttattccat agaattgaat aatgaaattg
      901 ccagtgtttg gtgtccatat tcattttttc tggagcagaa cgggcataaa caattattac
      961 attgcggtgt taattcaatt gaattatacc tgagcgataa tggctggcaa attacacaaa
     1021 tcacagatac ccgcgaggag acaggatgca gagaaaatta attcaatcgc ctaacaactt
     1081 attttcgtaa ataaacgcat taacccgata ccaaaccagt gaataaaact attgataagc
     1141 ttttaactaa agatgtaatt ccggattttt taatcagaca aaatatcagg aaactattac
     1201 gtcagcggct gattgacgaa accaaaccca accaggaact ccaacaacag catctcatgc
     1261 gcattgttga tgaattaaaa aaatcgccaa tcgccattga aaccaaggcc gccaacgagc
     1321 aacactatga attaccaacg gcattttttc aattggtaat gggaaaacat atgaaatatt
     1381 ccagttgtta ttggaatgct tcaacccctg atttgacagc agcagaaaac ctagccttgg
     1441 aaaaaacatg tgcacatgcc gaattagaaa atggcatgaa gattttggaa ctgggttgcg
     1501 gatggggaag tttaaccatg tttatggctg caaaatttcc tgatgcacaa atcaccggag
     1561 tttccaattc ggctactcaa aaacaatata tcgataaaac atgtgccgaa agaggattaa
     1621 aaaatgtacg catcataact gcggacatga atgtattttc tttggatgaa caattcgacc
     1681 gtgttgtttc agtagaaatg tttgaacaca tgcgcaatta tgaattgtta atgcaaaaaa
     1741 ttgcgcgtat gttaacggct gaaggaaagt tatttgtaca tatcttcacg catcaattat
     1801 ttacgtatta tttcgatgta atagatgaaa gtgattggat gagcaaatat ttttttaccg
     1861 gaggaataat gccttcagat gatttattat tatattttaa taacgatttg aaaattcaac
     1921 atcattatca ttgggatggc acacattatg aaaaaacagc aaattgttgg ctggaaaaca
     1981 tggataaaaa ccgcaataca atcatgccaa ttttgtctga tacttatggt gccgaccagg
     2041 ctacaaagtg gtgggtatat tggcggatat tttttatggc ctgtgccgaa ctctggggct
     2101 acaataaggg tagagagtgg tttgttagtc actatttatt tgtaaaagct taattcacta
     2161 taatgcaaat tgctataata ggaacaggaa ttgcgggtat gggatgtgcg catcgtttac
     2221 ataagatgca tgatattact gtttatgaac aagataatta tattggtggt cataccaata
     2281 ccgtttatgt aaatgaaggg gaaaatcgca ttcccattga tacggggttt attgttttta
     2341 ataatgaaaa ttacccacat ttaaatgcac tgtttaacac aataaaagcg ccaataaaaa
     2401 aaacatctat gagtttcgct gcacatatcg tgcatgaaaa cattgagtat tgtgggagta
     2461 gtttagatgg cttatttgct cagcgtaaaa atattttctc accaagatat gtccgttttt
     2521 taatgcaatt gaatgctttt aataaggtat gcttagaagt gcttgaaaag tctgaactgc
     2581 aatccttatc aattgctgaa tatgtaaata gtaagggctg ggggaaagat ttattaaatt
     2641 ggtatatatt accaatgagt tctgctttgt ggagtacgcc tcccgatacc agtgcgcaat
     2701 ttcctgctct tacgttggtg cgctttttca aaaatcacgg atttttagga ttaaatacgc
     2761 agtttcaatg gtacactgta gatggtggta gtgaagaata taaaaaacga ttgattgctc
     2821 catttaaaga taacatacaa atcaatgcag gtgttgttgg agtgaaaaaa gtagaaggta
     2881 aaattgaggt gaaaacaaaa aacggtggca ctaaaatttt tgataaggtt ataattgcga
     2941 cgcatgctga tcaagcgctt aggatgttgc aaaacccaac ggaacatgaa acacaattat
     3001 tgtcgtgttt ttcctatcag caaaacaaag caactttgca tacagattcg gctttaatgc
     3061 cttctgttaa aaaagtatgg agcagttgga attatcgaac cgaattaatt gataaaaatt
     3121 tagtggcttc ctgcacatac tggatgaata gtttacagga tgtgtcgaaa caaaaaaatt
     3181 attttgtcac aattaatgac ccgggaacaa taaaaaagga agcaattatc aaggaaataa
     3241 tgtatgaaca tccggtattt accgttgatg caatgcaggc gcagcagcat ttatcgaagt
     3301 taaataaaaa tggacagctc tttttttgcg gaagttattt ccgatatggc tttcatgagg
     3361 atgccctgat gagtgccaat gccgtttgcg atttaattga aatgcaacaa taatgtgtta
     3421 acttacattt gattaaaatg gattcagctt tatataattg cttagtaatg cacaaccggt
     3481 tagctccaaa aaagcacagg tttcattacc atgtatttat gttttggctc gatattgata
     3541 agttggaaca aacaactcaa tcaagtaaat tatttgctta taataaacgt gggatttata
     3601 gtttttggga taccgaccat tttaaatatc ccgcaggtga tgcgcgaaat ggtttaacaa
     3661 caaggcaaaa gctcaatgat tatttgctgt ctgttggtat aaccaaaatg cctacccaag
     3721 tatttttaac cacgcatgtg cgcatgttgg gttatgtatt t
//
LOCUS       DAORKW010000363         3727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1295_length_3727_cov_4.711874, whole genome shotgun sequence.
ACCESSION   DAORKW010000363 DAORKW010000000
VERSION     DAORKW010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1295_length_3727_cov_4.711874"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLB46_12500"
     CDS             complement(<1..317)
                     /locus_tag="PLB46_12500"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRF77389.1"
                     /translation="MAEKITKRDINYSEWYNDLVIQSGLAENSAVRGCMVIKPHGWAI
                     WENMKSILDKKFKDTGHVNASFPLLIPKSFLEQEEGHAEGFAKECAVVTHYRLKTHPD
                     KKG"
     gene            414..1994
                     /locus_tag="PLB46_12505"
     CDS             414..1994
                     /locus_tag="PLB46_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77390.1"
                     /translation="MKLRYLPILAALGFLATSCATQTASQGSSTDDIYYSAGDDIPSE
                     VITTTTTTSSSSSSDAYSTYPGNSGSYEYTEPRQDDNEYRAGEEFATESEEYYDEDGN
                     TYIVNNYYDDNDYDDYYYTSSLYRYYYPNYGFGYYSHCYSPYYYDPFWGPSYGWNVGY
                     SWGWGGVYYGNCYPYGYGHYGYYDPWYNPYYGYGGYYGGYYGSYWNGYNNGYWNGYYD
                     GYYGNGYGYGYGYGDDYYGYNNNDYNYGPNTSTGSNTNTGMESAIVLNDGYGKQQNYE
                     QNHVLTDKGALVTTKGDINTNAGSIDIAANTDKPVFDDIKGGINTNDALASGNIVKPG
                     SIAIDQPVVAGGAVVKENTGVKVIKYYSADSKPSASVNTANAEVKSDRGARGNGYNTV
                     SKPVNSNNKPSGLDSKPSKGTYYTPSGDQGRQQGTVKQNTNQAPSKIYEQPKSNSGSK
                     NTYEQPKSNGNNKSYEQPSKPSRPTYEAPKSSGSSSGSKPTYNSGSSNRNSGSSSGTS
                     SKPSSSGSSAPKKRSGGN"
     gene            2026..3531
                     /locus_tag="PLB46_12510"
     CDS             2026..3531
                     /locus_tag="PLB46_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77391.1"
                     /translation="MYKFFSMVIATALSLNLVAQSDVDALRYSTLDFGGTARSLGSGN
                     AYGALGGDMSSFSMNPAGIGIYRGSEFVLTPGLLSINSESSYFGTNTVEDKYKFTINN
                     AGIVFASVKRGKENATSGWVTGAFGISYNRLANFNSSVLYTGYNEGSSLLNSYVDYLN
                     ANGGTNPGDVFDKDPFGAGLAWETYLINPSGADSTQYFPVVEGNVQQTKSIATQGGIG
                     ETAITFGGNYGNRLYVGATIGVPHISYIVSSVYTESDVNGEYDNFESFTLTDVSETYG
                     TGINAKLGLIYRINDNLRLGAAVHSPTLYGLNDNYISSMSSNLEGDGEYEFDSPIGEF
                     SYELVTPWRVIGSAAVTIKNIGFISAEYEFVDYSESSYNFNRSLDAGDISYENTINTN
                     IDTKYGAASIIRLGGELTYDVFRFRGGYIMSSSPFNDGIAADGADFAKNTWTAGLGIK
                     EDDYFIDLAFAHSNTKEYDIQYVYDNGAGVNQGATIDKTFNNFLLSFGFRF"
BASE COUNT         1160 a          696 c          718 g         1153 t
ORIGIN      
        1 ttcccttttt tatcaggatg tgtttttaag cggtaatgcg taactacggc acactctttg
       61 gcaaagcctt cagcatgacc ttcttcctgc tcaagaaaac tttttggtat caacaaaggg
      121 aaactggcgt taacgtgtcc agtgtccttg aattttttgt ccagaatact tttcatattc
      181 tcccaaatcg cccatccatg gggttttatc accatacatc cccttactgc actgttctct
      241 gccaacccgc tctgtatcac aagatcgtta taccattctg aatagtttat atctcttttt
      301 gttatttttt cagccatatg ttatttttgg tatgattttg gttttaagtg atttgcaaaa
      361 ttaataattg cagccacagt aaacagattt aataaacaat taaaatatca gttatgaaat
      421 taagatacct tccaattctc gcagcgctgg gctttttagc tacttcctgt gctacccaga
      481 cagcatcaca gggatcttca actgacgata tttattattc tgcaggtgat gatatccctt
      541 cagaagtcat tacaaccaca accaccactt catcttcttc ttcatccgat gcgtattcta
      601 cttatccggg caattccggc agttatgaat acactgaacc acgtcaggat gataatgaat
      661 accgtgcagg tgaagaattt gccactgaat cagaagaata ttatgatgaa gatggtaaca
      721 cctacattgt aaacaactat tacgatgaca acgactatga tgattattac tatacatcta
      781 gtttgtatcg ttattattat ccaaactatg gtttcggata ttattctcat tgttattcac
      841 catactatta cgaccctttc tggggccctt catatggctg gaatgtaggc tatagctggg
      901 gttggggagg cgtatattac ggaaattgtt atccatacgg ttatggccat tatggttatt
      961 atgatccttg gtataaccct tattacggct acggcggata ttatggtgga tactatggca
     1021 gctactggaa cggttataac aatggttact ggaatggtta ttacgatggc tactacggta
     1081 atgggtatgg ttacggatat ggttacggtg atgactatta cggttataac aacaacgact
     1141 ataattatgg ccctaacact tcaaccggct cgaataccaa tacaggcatg gaatcagcaa
     1201 tcgtgttgaa tgatggttat ggtaaacaac aaaattatga gcaaaatcat gtgcttaccg
     1261 ataaaggtgc tttagttacc actaaaggtg acataaatac caacgcaggt tcaatcgata
     1321 ttgctgcaaa tactgataaa ccggtgtttg acgatattaa aggtggtatc aatactaacg
     1381 atgcattagc ttcaggtaat attgtaaaac cgggttcaat agcaattgat caaccggttg
     1441 ttgcaggcgg tgctgtggta aaagaaaata ccggcgttaa agtaattaaa tattatagtg
     1501 ccgatagcaa accttcagca tctgtaaata ctgcaaatgc agaagtaaaa tctgatcgag
     1561 gtgctcgcgg taatggatac aatactgttt caaaacctgt aaatagtaat aacaaaccat
     1621 caggcttaga cagcaaacca tctaaaggaa catattacac tccttcaggt gaccagggtc
     1681 gtcagcaagg tactgttaaa caaaatacca atcaggctcc ttcaaaaatt tatgagcagc
     1741 ctaaatcaaa tagtggaagc aaaaacactt atgaacaacc aaaatcaaat ggtaataata
     1801 agtcgtatga gcaaccatca aaaccatcac gcccaactta tgaagcgcca aaatcatcag
     1861 gttcttcatc tggtagcaaa ccaacttaca atagtggaag ttcgaatcgc aacagtgggt
     1921 cttcttcagg aacaagcagc aaaccatcta gcagtggaag cagcgctcct aaaaaaagaa
     1981 gtggcggtaa ttaatcagca ttaaaaaaca ctaaaaatta aaaccatgta taagtttttt
     2041 tcaatggtta tagctactgc tttgtcacta aatttagttg cacaaagtga tgttgatgcc
     2101 ttgcgatatt ccacactcga ttttggtgga actgcccgct cacttggttc cggaaatgca
     2161 tacggtgcat taggcggcga tatgagttcg tttagtatga accccgcagg tatcggaatt
     2221 tatcgcggct ccgaatttgt tttaacaccg ggcttattaa gcatcaatag cgagtcatct
     2281 tattttggaa ctaacacagt agaagataaa tataaattta ctattaataa tgcaggaatt
     2341 gtttttgcaa gtgtaaaacg tggtaaagaa aatgccacat ccggttgggt tacaggagca
     2401 tttggaattt catataatcg tttagccaat tttaatagct cggtattata caccggttat
     2461 aacgaaggct cttcattatt aaattcttac gtcgattatt taaatgctaa tggcggaact
     2521 aatcccggcg atgtgttcga caaagaccct tttggtgccg gacttgcatg ggaaacatat
     2581 ttaattaatc cttcaggtgc cgattcaaca caatattttc cggtagtgga aggaaatgtg
     2641 caacaaacaa aatcaattgc aacacaaggt ggaattggtg aaacagccat taccttcggt
     2701 ggaaattatg gcaatagatt atatgttggt gcaacaatcg gagttccgca tatttcttac
     2761 attgtttctt ctgtatatac cgaatcagat gtgaatggcg aatacgataa tttcgaatca
     2821 tttacattaa ccgatgtttc cgaaacttat ggaacaggta ttaatgcgaa attaggtttg
     2881 atttatcgaa ttaacgataa cctgcgttta ggtgctgctg tgcattcacc aacattatat
     2941 ggtttaaatg ataattatat ttcttccatg agcagcaatt tagaaggtga tggtgaatat
     3001 gaatttgatt ctccaattgg tgaatttagt tacgaattag tgactccatg gagagtaatt
     3061 ggaagcgctg ctgttactat caaaaatatt ggatttattt ctgcagaata tgagtttgta
     3121 gattacagtg aatcatcata taatttcaac cgttctttag atgccggtga tatttcttac
     3181 gagaatacaa ttaatacaaa tatcgatact aaatatggtg cagcatctat tattcgtctc
     3241 ggtggagaat taacttacga tgtattccgc ttcagaggtg gttatattat gagcagctca
     3301 ccgtttaacg atggcattgc tgccgacgga gccgatttcg caaaaaatac ttggaccgca
     3361 ggacttggaa ttaaagaaga tgattatttt attgaccttg catttgctca cagcaataca
     3421 aaagaatacg atattcaata tgtatacgat aacggcgccg gtgtaaacca aggtgcaact
     3481 atcgataaaa cttttaataa tttcctttta agttttggtt ttaggtttta atgattgtca
     3541 gtgaagcaaa gccctcctgg tggttcggga gggcttcttt attttcgtaa atgccgtaaa
     3601 tatatcattc aacaaaatat tttttttgga gcataaactc ctcacttcgt atggcaatcc
     3661 caacccgctt tccgctacaa cgaactaatg tgcgtgttca aattttaatt gataatctca
     3721 ctccatc
//
LOCUS       DAORKW010000364         3711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1303_length_3711_cov_3.929431, whole genome shotgun sequence.
ACCESSION   DAORKW010000364 DAORKW010000000
VERSION     DAORKW010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1303_length_3711_cov_3.929431"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="PLB46_12515"
     CDS             complement(<1..558)
                     /locus_tag="PLB46_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77392.1"
                     /translation="MRGIIKIAGLFLLFFSFSTRVFATHNRAGEITYEHIEDLTYLFT
                     ITTYTDPTSLADRDELTIDWGDETTEVVPRSSIEILVPGKIQKNEYTAYHTYGGAYTY
                     IVSMVDPNRVENILNIDGSVNVPFCLIDTVVIFDPLTYGFNSSPTLLYPPIDYANIGE
                     WFIHNPGAFDPDGDSLSYELVVPLQY"
     gene            971..1900
                     /locus_tag="PLB46_12520"
     CDS             971..1900
                     /locus_tag="PLB46_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77393.1"
                     /translation="MKKLPLLLPLALPFLLFNACAVEEEPDLPQGTFSSIFSFMESKK
                     SAPQTFTITSGSESTVIGEKGTILTFAPNSFVFANGTPCTGFITITLLEIQTNADMIY
                     NDVFPISGENILNSGGMYYVAASQGGNTLKLAPGATYNAVIPAQTNDPGMAFYSGVTT
                     PTGVDWVLIPPNMAAIPPIDFVQFNDLENTYSIFCDSVGWCNCDYVVGGPSVDCTIKL
                     DSNYTIGFDNAMGFAVLEDAHSVVSLNNPLANTVLINGIGAISMHFLIIAVLDGELYY
                     GLRPVIPEMDATYYIDLLPITEEDLDSLILGLP"
     gene            complement(1975..2733)
                     /locus_tag="PLB46_12525"
     CDS             complement(1975..2733)
                     /locus_tag="PLB46_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRF77394.1"
                     /translation="MNIKLRIWRQANAQAEGKLVEYQLNDVSANMSFLEMMDVLNEQL
                     ISKGETPVAFDHDCREGICGTCSMYINGRAHGPQKLSTTCQLYMRNFTDGETITIEPF
                     RAKGFPVLKDLCVDRSAFDRIIAAGGYISASTGQAQDANAIPVEKAAADSAMDAAACI
                     GCGACVAACKNASAMLFVSAKVSHLALLPQGHPERKKRALAMVAQMDAEGFGSCTNTY
                     ACEAECPKGISVANIARLNREFLNASLISEEMPK"
     gene            complement(2768..>3711)
                     /locus_tag="PLB46_12530"
     CDS             complement(2768..>3711)
                     /locus_tag="PLB46_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF77395.1"
                     /translation="GNLAPRDISSRAAKEACDDGRGVGESGLAVYLDFADAIKRLGKH
                     VIEERYGNLFEMYSHITAEDPYTVPMRIYPAVHYTMGGLWVDYELMTTVPGLYALGEA
                     NFSDHGANRLGASALMQGLADGYFVIPYTIGSYLSGEIFTKAIPTDHPAFVEAENHVQ
                     TTLNNLLSIQGTKSVDQYHKELGKIMWDYCGMARTAEGLTKARDMVRKLRAEFWQNVR
                     VTGKAEELNQDLEKAQRVADFMELGELMIVDALERNESCGGHFRVEYQEEGEAKRNDD
                     TFAYVAAWEFKGIDNNPVMHKEELKFEEVKLATRSYK"
BASE COUNT         1113 a          758 c          672 g         1168 t
ORIGIN      
        1 atactgcaaa ggcactacaa gttcataact taatgaatca ccatccgggt caaatgcacc
       61 gggattatga ataaaccatt caccgatatt ggcatagtca ataggcgggt ataacagcgt
      121 tggtgaactg ttaaatccat aagtaagggg gtcgaaaatt accacggtgt caattaaaca
      181 aaatggcaca ttcaccgaac catcaatatt tagaatattt tccaccctgt tagggtccac
      241 catgcttaca atataggtat atgctccacc atacgtatgg taggctgtgt attcattttt
      301 ttgaatttta ccgggaacca aaatttcaat cgaagagcga ggtaccactt cggtagtctc
      361 atcaccccaa tctatggtaa gttcatcgcg gtcagccaaa gaggttgggt cggtataggt
      421 ggtaattgtg aacagataag taaggtcctc aatatgctca taggtaattt ctccggcgcg
      481 gttatgcgta gcaaataccc tcgtggagaa ggagaaaaaa agtaaaaaca gccctgcaat
      541 tttgattata ccccgcattt cattgttttc agacctaaat ttacgaaaaa aacaggtgct
      601 ttattgtaaa ccctgagtaa aagttttgca atatcaaacc tcctcaataa aaattgttga
      661 catgctagat gaggtgacgt ttcgtttaat atgccaaaat acttcttgaa gacttctcta
      721 gtttaaattc ctctaaaagc attaacacgt caattatgat taaccagatg atttatattc
      781 ggtatttata atcacaccaa aaaaattaat tagtcccaat ttctgaaata aaaaaaatga
      841 gtatatttga tacacagagc aaactaacct aatcattgtg gcaaatcgtg aatgccacga
      901 tgaaacattt ttcccaatta agcgcaccac attttggaaa atttttaatt catttttgaa
      961 attaatcatt atgaaaaaat tacccttatt gctgccgttg gcgttgccct tcttgttatt
     1021 taatgcatgt gctgtagaag aagaaccgga tttgccgcaa ggtacattca gtagtatttt
     1081 ttcgttcatg gaaagcaaaa aatctgcacc ccaaacgttc actattacct caggttcgga
     1141 atctactgta attggcgaaa agggaaccat actaaccttt gcacccaatt cttttgtatt
     1201 tgccaacggt acaccttgta caggatttat taccatcaca ttattagaaa tacaaacaaa
     1261 tgccgacatg atttacaacg atgtgtttcc aataagtggt gaaaacatac tcaatagtgg
     1321 cggcatgtat tatgttgcag caagccaggg aggtaataca ttaaaacttg ctcccggtgc
     1381 aacttataat gctgtaattc ctgctcagac taatgatccg ggtatggcat tttattccgg
     1441 agtaactaca ccaacaggtg tcgactgggt actaattcct ccaaatatgg ctgcaatccc
     1501 accaatcgac tttgtgcaat ttaatgattt agaaaatacc tattccattt tttgtgatag
     1561 cgtaggatgg tgtaattgtg attatgttgt aggtggtcca tcagtagatt gtacaattaa
     1621 attggacagc aactatacta ttggatttga taatgctatg ggttttgcag tgctagagga
     1681 tgctcattca gttgtatctt taaataatcc tttagcaaat actgttttaa ttaatggcat
     1741 tggtgcaatt agtatgcatt ttttaattat tgctgtgtta gatggtgaat tatattatgg
     1801 gttaagaccg gtaattccgg aaatggatgc cacatattac atagatttat taccaattac
     1861 cgaagaagac ctggatagtt taattctggg gttaccttga taccaaattt ataaaataac
     1921 aaagccttgc atcgtaactt gcaaggcttt tgttttaact caaatcgaaa tcaattattt
     1981 gggcatctct tcactaatta aagaggcatt taaaaattct cggtttaagc gtgcaatatt
     2041 cgcaactgaa attcctttag gacattctgc ttcacatgcg taagtattag tgcatgaacc
     2101 aaatccttca gcatccattt gtgccaccat tgctaatgcg cgttttttgc gttcagggtg
     2161 tccttgtggt aataatgcta aatgcgaaac ttttgctgaa acaaacaaca tggctgatgc
     2221 atttttacaa gctgcaacac aagcgccgca acctatacat gctgcagcat ccattgccga
     2281 atcggcagct gctttttcaa ccggaattgc attggcatcc tgcgcctgtc cagttgatgc
     2341 agaaatatat ccgccggcag caataatacg gtcaaatgcc gaacggtcaa cacataaatc
     2401 ttttaatacc ggaaaacctt ttgcacgaaa tggttcaatg gtaatggttt caccatcggt
     2461 aaaattgcgc atgtataact gacaggtagt cgataatttt tgaggaccat gcgcacggcc
     2521 atttatatac atactgcatg ttccacaaat accttcacgg caatcgtggt cgaatgcaac
     2581 cggtgtttca ccttttgaaa tgagttgctc atttaataca tccatcattt ctaaaaacga
     2641 catattcgca cttacatcgt taagttggta ttctaccaat ttaccttctg cctgtgcatt
     2701 tgcttgtctc cagattctga gtttaatatt catagtcatt gatattttgt cattaacaat
     2761 tccgctttta tttataagaa cgggttgcca attttacttc ttcaaatttt aattcttctt
     2821 tatgcattac aggattattg tcgatgccct taaattccca ggcagcaaca taggcaaaag
     2881 tatcatcgtt acgttttgct tcgccttctt cctggtattc aacacggaaa tgtccaccgc
     2941 atgattcgtt gcgttctaat gcatctacaa tcattaattc gcctaattcc ataaagtccg
     3001 caacgcgttg tgctttttct aaatcctgat ttaactcttc agctttaccg gtaacacgaa
     3061 cattttgcca gaactctgca cgtaatttac gcaccatatc tctggctttt gttaagcctt
     3121 ctgcagtgcg cgccataccg caataatccc acataatttt gcctaattct ttgtggtatt
     3181 ggtcaaccga tttagtgccc tgtattgaca ataaattatt taatgttgtt tgaacatgat
     3241 tttctgcttc aacaaatgca gggtgatcgg ttggaattgc ctttgtaaat atttcgccgg
     3301 ataaataaga accaatggta tacggaatta caaagtaacc atctgccaat ccttgcatta
     3361 atgcacttgc acctaatcgg ttggcaccat ggtcgctgaa attagcttca cctaaagcat
     3421 ataatcccgg aactgttgtc attaattcat agtccaccca taatccaccc atggtataat
     3481 gcacggcagg gtatatacgc ataggcactg tataagggtc ttctgcagta atatgggaat
     3541 acatctcaaa taaattgccg tagcgttctt caattacatg tttgcctaaa cgtttaatgg
     3601 catcggcaaa atcgaggtaa acggctaaac cactttctcc cactcctctt ccatcatcgc
     3661 atgcttcttt cgctgcacga gatgaaatat cgcgaggtgc gagattaccg t
//
LOCUS       DAORKW010000365         3709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1304_length_3709_cov_6.172414, whole genome shotgun sequence.
ACCESSION   DAORKW010000365 DAORKW010000000
VERSION     DAORKW010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1304_length_3709_cov_6.172414"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLB46_12535"
     CDS             <1..511
                     /locus_tag="PLB46_12535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77396.1"
                     /translation="AALPADCDVKLYNQSVVQKGVSENGGTTDEQIIWNIGSAGTRYV
                     QVYGYSGAFNTSDCYDLLISVRSTNWKDDGTSALVEDEWNNSILGVFPNPATGDMVTV
                     DYFSVQEDMNVTVKVVDILGRQVGESIAAVTPGENMIDVNIQSLEAGFYFVVISNGKS
                     NYSEKFVVE"
     gene            672..2273
                     /locus_tag="PLB46_12540"
     CDS             672..2273
                     /locus_tag="PLB46_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77397.1"
                     /translation="MQTRKRIFYTFTFIFLFGLLACNKHYFRSNYRDANSLIHATSLL
                     PMQPFLKAHIKNGDVCILTNNWKIDNKQKTVSGSGVKYDFNRHLIFEGELIIPIDSVA
                     IFETNTKLNKNESDRIAALSILASADLALGVFCITTPKACFGSCPTFYLQPDQNFHYA
                     DAEGFSNAIAPSMEYFDIDALNNTKIIDGNFSITMKNEALETHCVNDVKLLAVPKKNN
                     QTVYQSAKNEFFVCENFYKPTQALAQEGDITNLLSAKDLQERFSGADDNNLSAKESII
                     IDFKQVDTQQDLGLVINFRQTLMTTYFIYSAMGYMGDQVGDMFALLESDENIKQQLSG
                     GIKKELGDIDVYLWIESDKKWELQSGLFETGPIAVNQQIIKINNKEKNTNPKIKIVLN
                     KGLWRIDNVALTNIMSTVTPLSISPTAIFNKGVADEHALNNLNDTSAYLISMPGDAFT
                     MQFQLPEKDTDYELFLYSKGYYLEWMRSHWIKDKDLQMLHTMLFNPKKYLKMEAANYK
                     KYETGMEADFWNSKIDTENFSYYEN"
     gene            2263..2760
                     /locus_tag="PLB46_12545"
     CDS             2263..2760
                     /locus_tag="PLB46_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77398.1"
                     /translation="MKTNLIIFAIAISLLCSCATPHYLPEAENIPSSQYGANIKVYRF
                     KAQAIIQGELIAVDSTKIIVRSDATHLCREIPRSEIQRYLVRYAEGYHYGWAIPTYAL
                     ATISHGFLLVITMPVNILVTSIITGNAENEFTYKEKHLPYEQLNMFARFPQGIPPNIK
                     LEDIK"
     gene            2827..>3709
                     /locus_tag="PLB46_12550"
     CDS             2827..>3709
                     /locus_tag="PLB46_12550"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRF77399.1"
                     /translation="MEGLQDQFAAIESLMTDVKAFPVANAAELEQFRIKFLGTKNVLK
                     DVFAEIKNVPNERKKEFGQKVNELKQLAEQRFAELEVVYKNAGNTTTGNIDISMPANT
                     TAPGARHPITLMKDELMAIFTKIGYTAIDGPEIEDDWHNFTALGMPEDHPARDMQDTF
                     YVQVNPAVLLRTHTSNVQIRVMEELTKSGKQPPVRIVALGRTYRNETISARAHCTFHQ
                     IELLYIDEGVTFADMKQTLLYLAQELYGAQTKIRLRPSYFPFTEPSAEMDVSCFVCGG
                     SGCAICKHTGWVEIGGCG"
BASE COUNT         1227 a          653 c          697 g         1132 t
ORIGIN      
        1 ggctgcatta cccgcggact gcgatgttaa attatataac caatctgtag ttcaaaaagg
       61 tgtgtctgaa aacggtggta ctacagatga acaaattatt tggaacattg gtagcgctgg
      121 aactcgttat gttcaggttt atggttattc aggagcattc aacacaagcg attgctatga
      181 tttattaatt tcagttcgtt ctacaaactg gaaagatgat ggcacttcag ctttagttga
      241 agatgaatgg aataactcaa ttcttggtgt attccctaac cctgctacag gtgatatggt
      301 tactgttgat tacttcagcg ttcaggaaga tatgaatgtt actgttaaag ttgttgatat
      361 cctcggccgt caggttggtg aatcaattgc tgctgtaact ccaggtgaaa atatgattga
      421 tgttaacatc caatcattag aagctggctt ctactttgtt gttatcagca atggtaaatc
      481 aaactactca gaaaaatttg tagttgaata attacaatta atatatttga aacggctgct
      541 ttattagcag ccgttttttt ttgcccataa tcgttggttt attatcatca aaccaacttt
      601 gtgatttatt ttgtagcttt atctattaac gcttgtttta tgttaatgcg attttattaa
      661 aacgaataac aatgcaaacc cggaagcgca tcttttacac tttcactttt atttttttat
      721 ttggattatt agcttgtaac aaacattatt tccgttccaa ttatcgtgat gcaaatagtt
      781 tgatacatgc aacttcttta ctgcctatgc aacctttttt aaaggcgcat attaaaaatg
      841 gggatgtgtg tatattaacg aataactgga aaatagataa taaacaaaag acggtttcag
      901 gttcgggtgt aaaatatgat tttaacagac acctcatttt tgaaggagag ctaataattc
      961 caatcgacag tgtggctatt ttcgaaacca atacgaagct gaataaaaat gaatcagaca
     1021 gaatcgccgc actaagtata ttagcaagtg ctgatcttgc ccttggtgta ttttgtataa
     1081 ccacacctaa agcgtgcttc ggttcctgtc ctacctttta tttgcaacct gatcaaaatt
     1141 ttcattatgc cgatgccgaa ggtttttcaa atgccattgc accaagtatg gaatactttg
     1201 atattgatgc attaaacaat actaaaatta tagatggtaa ttttagtatt accatgaaaa
     1261 atgaagcact agaaacacat tgcgtaaatg atgtcaaatt gcttgcagtg cctaaaaaaa
     1321 ataatcagac cgtttaccaa agcgcgaaaa acgaattttt tgtctgtgaa aatttttaca
     1381 aaccaaccca ggcgcttgct caggaaggtg atatcaccaa cttattatct gcaaaagatt
     1441 tacaggaacg attttccggc gctgatgaca ataatttaag tgctaaggaa tccattatta
     1501 tcgattttaa acaggtagat acacaacaag atttgggact ggtaattaat ttcagacaaa
     1561 cactcatgac cacctatttt atatattcag caatgggtta tatgggcgac caggttggcg
     1621 atatgtttgc tttattggag tctgacgaaa atattaaaca acaattaagt ggcggaatta
     1681 aaaaagaact aggtgatatt gatgtttatt tgtggattga atctgataaa aaatgggaat
     1741 tacaatctgg tttatttgaa acaggaccta ttgcagttaa tcagcaaata ataaaaatta
     1801 ataacaaaga aaaaaatacc aatccaaaaa ttaaaattgt tttaaataaa ggactttggc
     1861 gtattgataa tgtagcatta acgaatatta tgtcaaccgt aactccattg tcaataagcc
     1921 caacagctat attcaataaa ggtgtagccg atgaacacgc attaaataat ctgaacgata
     1981 cttcggcata tttaatctcg atgccgggag atgcctttac tatgcaattt caattacctg
     2041 aaaaagatac cgactatgaa ttatttttat attcaaaagg atattatctc gagtggatgc
     2101 gttcgcactg gataaaagat aaagacctgc aaatgttgca taccatgctg tttaatccaa
     2161 aaaaatattt aaaaatggaa gcagctaatt ataaaaaata cgaaacaggt atggaagctg
     2221 atttttggaa ttcaaaaatt gatactgaaa atttttccta ttatgaaaac taacttaatt
     2281 attttcgcta ttgccatttc attattgtgc agttgtgcta cgccacatta tttaccggaa
     2341 gcagaaaata tccccagcag tcaatacgga gctaatatta aagtataccg ctttaaagcc
     2401 caggcaatta ttcagggcga acttattgca gttgattcta ccaaaataat tgtaagaagt
     2461 gacgctaccc atttgtgtcg cgaaattcct agaagtgaaa tacaaagata tcttgtaaga
     2521 tatgcggaag gataccatta cggatgggca attccaacct atgctttagc aactatttca
     2581 catgggtttc tattggttat tacaatgcct gttaatattt tggttacttc aataattacc
     2641 ggcaatgcgg agaatgaatt cacctataag gaaaaacatc taccctacga acaattaaat
     2701 atgtttgcca gatttccaca gggcatacca cccaatatca agctggaaga cattaaatag
     2761 ttcagcaaaa gcgtccttgc gagttgccac cttcagcctc gttacttttt tcgtaaattt
     2821 gcccgcatgg aaggcttaca ggatcaattt gctgccatag aatcgctgat gacggatgtc
     2881 aaggcgtttc ctgtcgcaaa tgccgcagaa cttgaacaat tcagaattaa atttttgggt
     2941 actaaaaacg tattgaaaga tgtttttgca gaaattaaaa atgtacccaa tgaacggaaa
     3001 aaggaattcg gacaaaaagt aaacgagctc aaacaattgg cagaacaacg ttttgctgaa
     3061 ttggaggttg tttataaaaa tgcagggaac acaaccacag gcaatatcga tatcagtatg
     3121 cctgccaata caaccgctcc cggcgcccga catccaatta ctttaatgaa agacgagtta
     3181 atggccattt ttaccaaaat aggatatacg gctattgatg gacctgaaat tgaagacgat
     3241 tggcacaact ttactgccct tggaatgccg gaagaccatc ctgcccgcga tatgcaggat
     3301 acgttttatg tgcaggttaa ccctgctgta ttattgcgca cacatacttc taatgtgcag
     3361 attagagtaa tggaagaact caccaaatct ggtaaacaac ctcctgtgcg tattgtggct
     3421 cttggcagaa cttatcgcaa tgaaactata tcagcacgcg cgcattgcac attccatcaa
     3481 atagaattat tgtatataga cgaaggggta acctttgccg atatgaaaca aacactcctt
     3541 tatcttgctc aggaattata cggagcgcaa acaaaaattc gtttacgtcc cagttatttt
     3601 ccattcaccg agccaagtgc tgaaatggat gtaagttgtt tcgtttgtgg cggaagtggt
     3661 tgcgcaatct gtaaacacac cggatgggtt gaaattggcg gttgtggaa
//
LOCUS       DAORKW010000366         3709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1305_length_3709_cov_4.096059, whole genome shotgun sequence.
ACCESSION   DAORKW010000366 DAORKW010000000
VERSION     DAORKW010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1305_length_3709_cov_4.096059"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="PLB46_12555"
     CDS             complement(<1..1454)
                     /locus_tag="PLB46_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRF77400.1"
                     /translation="MRALKSVNKYLWRYRWLLLTGILFVGLANVFQVYAPRYIGEMID
                     LVTVNFDAVVKQNDTTLANEVYRGVLISGLLFLGFTILRGIFMFFMRQTIIVMSRKIE
                     YDQKNDLFRHYEQLTPAFFKKNNTGDLMSRVAEDVGRVRQYVGPALMYFMNLVFNFLF
                     GIWMMLSINPTLTLYVLLPLPVLSLSIYYVNSIINKRSEAIQTQLSHLTSVAQESFSG
                     IRVIQAYAREKTTALYFEKECDEYKQLSLGLARVDALFQPLMVLLIGLSVLLTIFVGG
                     WQVMEGKLSTGDLAEFLFYVNMMTWPVTSLGWVVSIIQRAAASQKRIDEFLNTQPDVA
                     ATGFPHHTLKGAIVFDDVHFTYPDTGVKALQGVSFSILPGEKIAIVGKTGRGKTTIAD
                     LMYRLYDVSSGQIRIDGIPIDAFNLVDLRKGISAVPQDVFLFSDSIKNNIAFGNGKTE
                     LATVQQYAAYASIHDEITQFREGYDTLVGARGVTL"
     gene            1547..2644
                     /locus_tag="PLB46_12560"
     CDS             1547..2644
                     /locus_tag="PLB46_12560"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRF77401.1"
                     /translation="MSNTSLQLTNGEIFSTMMEMQHEQMVFCTDKTTGLKAIIAIHNT
                     TLGSALGGTRFWNYTNEDDAIIDVLRLSRGMTYKAAISGLNLGGGKAVIIGDSKALAN
                     REPLFRRYGKFVNSLNGKYITAEDVGTSTGDMEYINMETDYVAGKPEQLGGGGDPSPV
                     TAYGVYLGMKAAAKYANGTDNLKGKKILVQGVGNVGYHLVERLVKEEAIVMISDISED
                     RIKKVTQEHHVQVIDKDTIFETDMDIYAPCALGATINDDTIGKLKCNIVAGAANNQLK
                     DEQKHGMALIERGILYAPDYLINAGGLINCYSELEGYNRERAMQKTEIIYPRALEIFE
                     IAAKEKITTQAAANRIAEERIRLVAHINSRL"
     gene            2779..>3709
                     /gene="nusB"
                     /locus_tag="PLB46_12565"
     CDS             2779..>3709
                     /gene="nusB"
                     /locus_tag="PLB46_12565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRF77402.1"
                     /translation="MLSRRGLRIKAMQTLYIVASTENMDSRMAVKQLHKSIYNSHLAY
                     LYCLHFITELAQQVDLEAHIKKNKYLPTDSDLNFPTTFFNNTLITSLISNTYFQNRLK
                     KEKLLDWADEEYAKIIYNQLKEFPPYIKYAESQPDAAQDKKIVKEIFESFLLKNEYFD
                     EHLENVIGTWADDEFIVKGLMDDFFNAKHIGPDNERQLFDFYITAEEEDFSETLLNKT
                     ISEDSNYDEMIKPKLQNWELERVSLIDKILMKMALTEFIHIPTVPTKVSINEYLDISK
                     LYSTPRSREFINGILDKSMNEMKSSGKIIKTGRG"
BASE COUNT         1223 a          738 c          705 g         1043 t
ORIGIN      
        1 agcgttactc ctcttgcccc taccagcgta tcataccctt ctctgaactg ggtaatttca
       61 tcatgaatcg aagcataagc tgcatattgc tgcacggtag cgagctcagt tttcccatta
      121 ccgaatgcga tattgttttt aatactatcc gaaaacaaaa acacatcctg tggcactgcc
      181 gaaatgcctt tgcgcaaatc aaccaggttg aaagcatcta tcggaatacc atcaatacgt
      241 atttgtccgg aactaacatc atataatcga tacatcaaat cagcaatggt tgttttgcca
      301 cgtcccgttt tgcccactat ggcaattttt tcgcccggca aaatgctaaa actcacaccc
      361 tgtaaggctt tcactccagt atccggatag gtaaaatgta catcgtcaaa aacaatagcg
      421 ccttttaagg tatgatgagg aaacccggta gcagctacat caggctgcgt attgaggaat
      481 tcatcaattc gtttttgact tgctgcagct cgttgtataa tagaaacaac ccatccgagg
      541 gaagtaaccg gccaggtcat catatttaca taaaacaaaa attccgcgag gtcacccgtg
      601 cttaatttgc cttccattac ctgccatccc cccacaaaaa tggttaacaa aacgctcaac
      661 ccgattaaca gcaccataag tggttgaaaa agcgcatcta cccttgccaa tcccaacgaa
      721 agttgtttgt attcatcaca ttctttttca aaatataagg cagtagtttt ttccctggca
      781 taggcttgaa ttaccctgat gcctgaaaag gactcctggg ccacacttgt taaatgggat
      841 aactgcgtct ggatagcctc actgcgtttg ttgatgatac tgtttacata ataaatggaa
      901 agggacaaca caggaagtgg caacaacaca tatagggtaa gtgttgggtt aatactgagc
      961 atcatccata ttccgaaaag gaagttaaat accaggttca taaaatacat cagcgctggg
     1021 ccaacatatt gccttactct tccaacatcc tctgctaccc tgctcatgag gtcgccggta
     1081 ttattttttt tgaaaaaagc cggtgtcagc tgctcataat ggcgaaacag gtcgtttttt
     1141 tggtcgtatt caatcttgcg gctcattaca atgatagttt gccgcataaa aaacatgaaa
     1201 atgcccctta aaatggtaaa accgagaaat aataagccgc taataagcac tccacgataa
     1261 acttcattcg ccagtgtagt gtcattttgt ttgacaacag catcaaaatt tacggtgaca
     1321 aggtctatca tttcaccaat ataccttggg gcgtatacct gaaaaacatt ggctaaaccg
     1381 acaaacaaaa tgcccgtaag caagagccac ctatacctcc acaaatattt attaaccgac
     1441 ttcagcgcac gcatataaaa tgcaaagcta taaatcaatt gaaaagtttc tactctttat
     1501 aacttcttct atctttgcgg aaccaattta aaaacaacgt tcatttatgt caaatacttc
     1561 tctgcagctg accaatggtg aaattttctc tacaatgatg gagatgcaac acgaacaaat
     1621 ggtcttctgt accgataaaa ccaccggcct taaggctatt atagcaattc ataacaccac
     1681 acttgggtct gctttaggtg gcactcgctt ctggaattac accaatgaag acgatgcaat
     1741 tattgatgtt ttgcgtttat cgcgcggaat gacctataaa gctgctatta gtggtttgaa
     1801 cttaggtggt ggcaaagctg tcatcattgg cgactcaaaa gcgcttgcga accgtgaacc
     1861 attatttcgt cgttatggca agtttgtaaa ttccctcaac ggaaaatata ttactgctga
     1921 agatgtagga acatctaccg gcgacatgga atacatcaac atggaaaccg actacgttgc
     1981 cggtaaacct gaacaattag gcggaggtgg cgatccatca cctgtaacag cttacggtgt
     2041 ttatcttgga atgaaagctg cggcaaaata tgccaatggc actgataatt taaaaggcaa
     2101 aaagatttta gttcagggtg tcggtaatgt tgggtatcac ttagtggaac gtttggtgaa
     2161 agaagaagct atagtgatga tttctgatat tagtgaagac agaattaaaa aagttacaca
     2221 ggaacatcac gttcaagtta ttgataaaga taccattttc gaaaccgata tggacattta
     2281 tgcgccatgt gctttgggtg ctaccattaa tgatgatact attggtaaac tgaaatgtaa
     2341 tatcgttgct ggtgctgcga acaatcagtt aaaagatgag caaaaacatg gtatggcttt
     2401 aattgaacgc ggcattttat atgcaccgga ctatttaatc aacgccggcg gtttgattaa
     2461 ttgttattct gaattagaag gatataacag agaacgcgcc atgcaaaaaa cagaaattat
     2521 ttatccaaga gcattagaaa tttttgaaat tgctgcgaaa gaaaaaataa ccacacaggc
     2581 tgctgccaac agaattgcag aggagcgtat tcgcttggtt gcgcatatca acagccgcct
     2641 ttgataaaaa caatatataa cggtataagt cgtcacttta cttataccgt tattttttaa
     2701 actataaata ttattaattc aacaattgcg gaatttaccg cgtcaggcaa aataccaaac
     2761 taataactgt taattgacat gctgagtaga agaggattac gtatcaaggc aatgcaaact
     2821 ttatatatcg ttgccagtac agagaatatg gatagcagaa tggctgtcaa acaattacat
     2881 aaaagtatat acaattcgca tttagcgtat ttatactgcc tgcattttat cactgagctc
     2941 gctcaacagg ttgacttaga ggcacatatc aaaaaaaaca agtatttacc tactgattct
     3001 gacctaaact ttcccactac gttttttaac aacaccctca ttaccagttt aatatctaat
     3061 acttactttc aaaatcggtt aaaaaaggaa aaattactcg attgggctga tgaggaatat
     3121 gccaaaataa tttacaacca gcttaaagaa ttcccacctt atattaagta tgcagaatca
     3181 cagccggatg ctgcacagga taaaaaaatt gtgaaagaaa ttttcgaaag ttttttattg
     3241 aagaatgaat atttcgatga acacttagaa aacgtaattg gcacctgggc tgatgatgag
     3301 tttattgtta aaggtttaat ggatgatttt tttaatgcca aacatattgg cccggataac
     3361 gaacgtcaat tatttgactt ctatattaca gcagaggaag aagatttttc ggaaacgctg
     3421 ttgaataaaa ccatttcgga ggacagcaat tacgacgaaa tgataaaacc taagctccaa
     3481 aactgggaat tggaacgtgt ttcgctgatt gataaaattt tgatgaaaat ggcgttaaca
     3541 gagtttattc acatccctac agtgccaaca aaagtgagta tcaacgaata tttggatatc
     3601 agcaagttat acagcacacc gagaagccgt gaatttatta acggtatttt ggacaagtcg
     3661 atgaatgaaa tgaaatcgtc cggcaaaatc attaaaacag gacgtggtt
//
LOCUS       DAORKW010000367         3699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1316_length_3699_cov_5.626784, whole genome shotgun sequence.
ACCESSION   DAORKW010000367 DAORKW010000000
VERSION     DAORKW010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1316_length_3699_cov_5.626784"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="PLB46_12570"
     CDS             <1..1750
                     /locus_tag="PLB46_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF77403.1"
                     /translation="GAEQEVCIVNEDFKPADNAIDLLNKINHPQFTTELAKYNIEINL
                     LPQLLKPGCFAAMENDLLEKFQLAANLAATNNTKLVLAGILPTISRNEISLDYLTPLE
                     RYHMLSKKLRDLRGRKFDLYLKGVDELHIRHDSIMFEACNTSFQTHLQIPPEEFVPAY
                     NWALAISAPVLAISSNSPILLGKELWSEIRIALFQQSIDTRHSIDEIREQRPRVTFGK
                     DWIYHSITEIYKEDITRFEVLINDEITENSVETIQNGGIPQLRALRLHNGTIYRWNRM
                     CYGITDNKPHMRIECRYIPAGPSIQDEIANAAFWVGLMKARPENVKKIWEHFDFKDVK
                     SNFFKAARSGVESVFVWRGQTISAHDLIKNELLPLAHEGLKNCGFSNEEIYVYLGTIE
                     KRLSSNTGSQWQVRNFRNLKKEMNVTDALTALTEFMHDQQQQNTAVCAWSDIQIPVAL
                     KKQVANMRTVKQLMSSDVITVFPDDPAALVDQIMRWHDVNHIVVEDRKGNLVGVVTSG
                     RIAEIAEINPLFGELPVKKIMRKDITTITPESEISEALEIMQNLWISSLPVVANNKLM
                     GIVTKNDMLRWMALKS"
     gene            1820..>3699
                     /locus_tag="PLB46_12575"
     CDS             1820..>3699
                     /locus_tag="PLB46_12575"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF77404.1"
                     /translation="MRITTIITVCCVTIMSCKQNTKAPEEDVQGPGKVTYPASLTTEA
                     NSDYFGTMVNDPYRWLEAEANEDSAVRGWVTAQNDVTFDYLAKIPYREQLEQRMTELA
                     DYPKMSTPYRSGEYYFYSKNDGLQNQSVTFYKKGLNGEEKVFLDPNKLSEDGTITQSM
                     SGFSNDRKYVAISESKAGSDWNTMYVKDVAKNEMLADKLEWTKFSGAAWQGDGFYYSA
                     YDKPSQTFTGTSENQKIFYHKLGTPQSSDKLIYTDPKNSKYYFGAQTTEDERYLFVYV
                     SPGTSGTEVLWKDLRKPNASFTKLFPGFDFEYTILDNDDDKLIVMTNHNAPNYKVVSV
                     DPTRPEPENWKVIIAEQKELLSTVSLAGGKLFAFYLRDVKPVIIQFSRTGDAERQIAL
                     PNNGIGGTASGFAGERDDDEVFFSFTTFLSPADIYKYNIQSGKVDMYKKSEIKFDASK
                     YTMKQVFYPSKDGTKIPMFIVHKKGMKMDGSNPCLLYGYGGFNISITPSFSISRIAML
                     EQGFVYAVANIRGGGEYGEEWHKAAMFEKKQNVFDDFIAAGEYLINEKYTSNTKLAIQ
                     GRSNGGLLVGACMTQRPDLFAVALPGVGVMDMLRYHKFTVGWGWAVEYGCADSSKEAF
                     DY"
BASE COUNT         1268 a          607 c          731 g         1093 t
ORIGIN      
        1 aggtgctgaa caggaggttt gtattgtaaa tgaagatttt aaaccggccg acaatgctat
       61 tgatttatta aataaaataa atcatccgca gtttactact gaactcgcaa agtataatat
      121 tgaaatcaat ctgttacctc agttattgaa acccggttgt tttgcagcta tggaaaacga
      181 cttgttggaa aaatttcaat tggcagcaaa tctggcagct accaacaata ccaaattagt
      241 attagcagga attttaccta ccatttcacg taatgaaatt tcattggatt atttaacgcc
      301 attggaacgt tatcatatgc tgagcaaaaa attacgtgat ttacgtggga gaaagttcga
      361 tttatattta aaaggtgttg atgaattaca tattcgacat gatagtatta tgttcgaagc
      421 atgcaatact agctttcaaa cacatttaca aatcccaccg gaagaatttg tccctgcata
      481 taattgggct ttagctattt ctgcaccggt actggccatc tcttctaatt cgcccatttt
      541 actcggaaaa gaattatgga gtgaaatcag aattgcatta tttcaacaaa gcatcgatac
      601 cagacatagc attgatgaaa ttagagagca acgtccgcgt gtaacatttg gtaaagattg
      661 gatttaccat tcaattactg aaatatataa agaggatatt acgcgttttg aagttttaat
      721 aaatgatgaa attaccgaaa attcagttga aacaattcaa aacggaggta taccacaatt
      781 gcgcgcattg cgtttacaca atggcactat ttacagatgg aacagaatgt gctacggcat
      841 taccgataac aagccgcaca tgcgtatcga atgtcgctat atccctgcag gaccaagcat
      901 tcaggacgaa attgccaatg ctgcattttg ggttggttta atgaaagcaa gaccggaaaa
      961 tgtaaaaaag atttgggaac attttgattt taaagatgta aaatccaact tttttaaagc
     1021 tgcgcgttca ggtgttgaaa gtgtttttgt ttggagggga caaaccattt cagctcatga
     1081 tttaataaaa aatgaattgc tgccattagc gcatgaagga ttaaaaaatt gcggtttcag
     1141 caatgaagaa atttatgtgt atttaggcac tattgaaaaa cgtttatcaa gtaacacagg
     1201 ctcacaatgg caagtaagaa atttccgcaa tctaaaaaag gaaatgaacg taactgatgc
     1261 attaactgcg cttactgaat ttatgcatga ccaacaacaa caaaacactg ctgtttgtgc
     1321 atggtctgat atacaaatcc ctgttgcgtt aaaaaaacag gtagctaata tgcgcacggt
     1381 aaaacaatta atgagttcag atgtaataac cgtttttccc gatgacccgg ctgcattggt
     1441 tgatcaaata atgcgttggc acgatgttaa tcatatcgtt gttgaggaca gaaaaggcaa
     1501 tttagttggt gttgtcacaa gtggtcgcat agctgaaatt gcagaaataa atccattatt
     1561 cggtgaattg cctgttaaaa aaataatgcg taaagatatt actacaataa cacctgaaag
     1621 tgaaatttcg gaggcactag aaattatgca aaatctttgg attagcagct tgccagttgt
     1681 agcaaacaat aaattaatgg gtattgtcac caaaaatgat atgttgcgtt ggatggcatt
     1741 aaaaagctga cgttaaaaac tgttataaat cctgatatac agctatattg aattaacttt
     1801 gttattaaat cacaacttta tgcgcataac cacaataatt accgtatgtt gtgtgactat
     1861 tatgtcatgc aagcaaaata ctaaagcacc ggaagaagat gttcaaggtc cgggcaaagt
     1921 aacttatccc gcatcattaa cgactgaagc caattctgat tacttcggca caatggtaaa
     1981 cgacccttac cgttggctgg aagctgaagc taacgaagat tctgccgtgc gcggttgggt
     2041 tacagctcaa aatgatgtaa cgtttgatta tctggcaaaa atcccatatc gtgagcaatt
     2101 agagcaacgt atgacagaac tggcagatta tcctaaaatg tctacgcctt accgctcagg
     2161 tgaatattat ttttatagta aaaatgatgg tctgcaaaat caaagtgtaa ccttttataa
     2221 aaaaggttta aacggagagg aaaaagtatt tcttgaccct aataaacttt ctgaagatgg
     2281 aacaattaca caatccatgt caggttttag taacgataga aaatatgtgg caattagtga
     2341 aagcaaagcc ggcagcgact ggaataccat gtatgtaaaa gatgttgcca aaaatgaaat
     2401 gttggctgat aaattagaat ggacaaaatt tagtggcgcc gcttggcagg gagatggatt
     2461 ttattatagc gcgtatgaca aaccatcaca aacatttaca ggcactagtg aaaaccaaaa
     2521 aatattctat cataaattag gtacaccaca aagttccgat aaattaattt ataccgaccc
     2581 aaaaaattct aaatattatt ttggtgcaca aactaccgaa gatgaacgat atttatttgt
     2641 atatgtaagt ccgggaacat caggcactga agttttatgg aaagatttgc gcaaaccaaa
     2701 tgcatcattt acaaaattat ttccgggatt cgattttgag tataccattt tagataatga
     2761 cgatgataag ttaattgtca tgactaatca caatgcacca aactacaaag tagtaagtgt
     2821 tgaccctaca cgtcctgaac cggaaaattg gaaagtaatt attgctgagc aaaaagaatt
     2881 attatcaacc gtttcattgg caggtggtaa attatttgcc ttttatttac gcgatgtaaa
     2941 accggtaatt attcagtttt cacgcacagg agatgctgaa agacaaattg cacttcctaa
     3001 caatggtatt ggtggaactg ccagtggttt tgccggtgaa cgcgacgacg acgaagtgtt
     3061 tttctcattt acaacattcc tttctccggc tgatatttat aaatacaata tccaatcagg
     3121 taaagttgat atgtataaga aatcagaaat aaaatttgat gcttctaagt acaccatgaa
     3181 acaagtattt tatccgagca aagacggcac aaaaatacct atgtttattg tgcacaaaaa
     3241 aggcatgaaa atggatggct caaatccatg tttattatat ggttatggtg gatttaatat
     3301 ttctattaca ccaagtttca gcatttcaag aattgctatg ttagaacagg gatttgtata
     3361 cgctgttgcc aacattagag gaggaggtga atatggagaa gaatggcata aagcggctat
     3421 gttcgaaaag aaacaaaatg tatttgatga ttttatagca gcaggtgaat atttaattaa
     3481 cgaaaaatat acttctaata cgaaactagc cattcaaggt cgctctaatg gcggattact
     3541 ggttggcgct tgtatgactc aacgtcctga tttatttgct gttgcacttc caggtgttgg
     3601 cgttatggat atgttgcgtt atcacaaatt taccgttggc tggggatggg ctgttgaata
     3661 tggttgtgcc gatagttcta aagaagcatt tgattattt
//
LOCUS       DAORKW010000368         3699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1317_length_3699_cov_5.050494, whole genome shotgun sequence.
ACCESSION   DAORKW010000368 DAORKW010000000
VERSION     DAORKW010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1317_length_3699_cov_5.050494"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PLB46_12580"
     CDS             complement(<1..992)
                     /locus_tag="PLB46_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77405.1"
                     /translation="MRRLLVFFIALTLFGKAQSQIVASPDTTICVCGTATLLIFSSPA
                     YGTSSYTFETMPYSPEVYGGTKVPKSGGGAGGAGLEDDTYSKPLDMGFSFCFLGEEYT
                     QCYVGSNGWLSFGGPGALVTTYTSEAIPSTDPDVPKNCIMGPWQDWHPGLCSPVGSCI
                     MYQTIGVAPNRKFVVSFDNVPMFSCTGLEGKFQIVLNETSNIIENHITNKPNCMGWAG
                     GTATQGVHSLDGLTAFTAPGRNSTPWTAANESTRFVPNGVVWYDPAGAIVGYGDSITV
                     SPTTTTTYTAVFVNCAGADVEEDITVFVEDPDPSFDYDLFYCIDGYATPTITGDP"
     assembly_gap    1212..1311
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1500..1573)
                     /locus_tag="PLB46_12585"
     tRNA            complement(1500..1573)
                     /locus_tag="PLB46_12585"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1537..1539),aa:Asn,seq:gtt)
     gene            1793..2710
                     /locus_tag="PLB46_12590"
     CDS             1793..2710
                     /locus_tag="PLB46_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRF77406.1"
                     /translation="MSLLVVGTVAFDAIETPFGKKDKIIGGSATYIGLSASYFYKKIK
                     LVSVVGSDFPKATIAQFNKKGINTDGIEVIKGKKSFFWSGRYHMDMNTRDTLVTDLNV
                     LADFNPVLPAASRKPDFLMLGNIEPSLQIKVIEQLKTRPQLIAMDTMNFWMDIARPNL
                     DKVLTMVDLLIINDEEARQLTGEYSLVKAAKKIMKTGPKTLVVKKGEHGALLFQGDQV
                     FFAPALPLEEVFDPTGAGDTFAGGFMGYLAKTKNISFTNMKRAIIYGSAMASFCVEKF
                     GPERLLDLKQSQVAERVKTFVQLTKFNIK"
     gene            complement(2880..3116)
                     /locus_tag="PLB46_12595"
     CDS             complement(2880..3116)
                     /locus_tag="PLB46_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HRF77407.1"
                     /translation="METLPEYNKFQLALRNGQDREEIWIAFKGIIYDVTTSRLWRKGQ
                     HYEHFAGQDLTHELADAPHTEIVFQRFPIIGKLI"
     gene            complement(3116..>3699)
                     /locus_tag="PLB46_12600"
     CDS             complement(3116..>3699)
                     /locus_tag="PLB46_12600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF77408.1"
                     /translation="LLYWKIPTNNEENNRLATTENLFLGDTKMVYQIPANQNLLPEIQ
                     EHVRSWNLPPNQQLISLGIASGEFSRFKTDPKMPEGFFEKMYAEWVVQSVQGTMGNVI
                     YYIGNETIIKGFITLQFHSDYAEIGLIAVDEKYRKQQIATQLIQFALDIAANHNLKNV
                     QVATQLANTPANNLYIKCGGTLITAENIYHIWL"
BASE COUNT         1126 a          649 c          732 g         1092 t
ORIGIN      
        1 ggatcaccgg taattgtagg cgtcgcgtag ccatcaatac aataaaacaa atcgtaatca
       61 aaagacgggt cagggtcttc aacgaatacg gttatgtctt cttcaacatc tgctcctgcg
      121 caattaacga aaacagctgt ataagtagtt gtggtggtag ggctaacagt aatgctgtct
      181 ccatacccaa caattgctcc ggcgggatcg taccaaacaa caccattagg tacaaagcga
      241 gtgctttcat ttgccgcagt ccaaggtgtt gagtttctac ccggtgctgt aaaagccgtt
      301 aaaccatcga ggctgtgaac accttgagtg gcagttccac cggcccaacc catacagttt
      361 ggtttattgg taatatggtt ttcgatgata ttggatgttt cgttaagaac aatttgaaat
      421 ttaccttcca aaccggtaca gctaaacatg ggaacattat caaaacttac cacaaatttt
      481 ctgttgggag caactcctat agtttgatac ataatgcatg aacctacagg tgaacataag
      541 cccggatgcc agtcctgcca tggccccata atacaatttt taggtacatc tgggtctgtt
      601 gaaggaattg cttcagaggt ataggtagta actaatgcac caggtccgcc aaagctgagc
      661 cagccattag agcccacata acattgggtg tattcttctc ctaaaaagca aaaagaaaat
      721 cccatgtcaa gtggtttact gtatgtatcg tcttcgaggc ctgcaccgcc tgcaccaccg
      781 ccggatttag ggacttttgt gcctccgtat acttccgggc tgtaaggcat agtttcaaat
      841 gtataacttg atgtaccgta agcaggagac gaaaatatga gtagggtagc tgttccacac
      901 acacaaatag tagtatcagg tgaagctaca atttgcgatt gtgctttgcc gaaaagggtt
      961 agagcaataa aaaagactaa taatctcctc attatcagct aatcggattg tgtgtttgta
     1021 aaaagtggtg caagttaagc aaataatgaa atataaaaaa gtgtgataaa aacactgtgt
     1081 ggattagcta aaaagggctt tgaacacgct tattcataaa ctgatagagc aatccagcgc
     1141 tcagaaaaaa tgccccaaaa tgcaaaaaac ccaccaattg gcaggttttt gctccccaac
     1201 ctgtcccgat annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagttgcata
     1321 aaatgcaaaa aacccaccaa ttggcaggtt tttgctcccc aacctgtccc gatagctatc
     1381 gggattgaac cagggaccta cttctttttt gggaaaaaat gggggtgtaa caaaaacacc
     1441 aacaatatgt attggaagtt gcataaaatg caaaaaaccc accaattggc aggtttttgc
     1501 tccccaacct ggacttgaac cagggaccta ctgattaaca gtcagtcgct ctaaccagct
     1561 gagctattga ggaatgtttc ttttgaaacg ggatgcaaat ttattttgtt atatccatac
     1621 aacaaagaat ttttgaaaat ttaattagaa tatttctttc ggggtttatt tgggaagtgc
     1681 aataagtgcc ggtttaaagg atattagacg atattttaaa aaaggactaa aaattaagat
     1741 aaaaagcaaa atgattagtc gagaagtgtt ttctttgcag caaaattaaa acatgagttt
     1801 attagtagtt ggaacagtcg catttgatgc aatagaaaca ccttttggaa aaaaggataa
     1861 aataataggt ggttcagcaa cctatatcgg attatctgcc tcgtattttt ataaaaaaat
     1921 aaaattggta tcggttgtag gaagtgattt ccctaaagca actatcgcac aatttaataa
     1981 aaagggtatc aatactgatg gtatcgaagt aataaaagga aaaaaatcgt ttttttggtc
     2041 gggacgttat cacatggata tgaacacccg cgacacgcta gttaccgatt taaatgtact
     2101 tgcggatttt aatccggttt taccggcagc atcacgcaag ccggattttt taatgttagg
     2161 taatattgaa ccttcattgc aaattaaagt gatagagcaa ttaaaaacgc gtccgcaatt
     2221 aattgctatg gataccatga atttttggat ggatattgct cgccctaatt tggataaggt
     2281 attaacgatg gttgaccttt tgataattaa tgatgaagag gcgcgtcaat taaccggtga
     2341 atattcgtta gtaaaggccg cgaaaaaaat aatgaaaaca ggacctaaaa cgctggttgt
     2401 aaaaaaaggt gaacacggtg cattattatt tcagggcgat caggtgtttt ttgctccggc
     2461 attaccatta gaagaagtat ttgacccaac aggtgcaggt gatacatttg caggcggatt
     2521 tatgggatat ttagcaaaaa caaaaaacat ttcatttact aatatgaaac gcgccattat
     2581 ttacggcagt gcaatggctt cgttttgtgt tgaaaaattt ggaccagaac gtttgttgga
     2641 tttaaaacaa tcgcaagttg cggagcgtgt taaaacgttt gtgcagttaa cgaagtttaa
     2701 tattaaatga tagaactcat ctcaaaaata cctttgtcgt cttttctgcg tcttcgctct
     2761 ttatccttcg ttgcttgaat tgcccgtact tcctccaata tgggctgctc caagcacctc
     2821 ggctaaagtg cgaatacttg aaaattctaa cgaaaaaata tttttgagat gagttctaat
     2881 caaattagtt ttccaatgat gggaaatcgt tggaaaacta tttcagtatg aggtgcatct
     2941 gcaagttcat gggtgaggtc ttgtcctgca aagtgttcgt agtgttgtcc tttacgccat
     3001 aacctactgg tggttacgtc ataaataatc cctttaaagg caatccatat ttcttcgcga
     3061 tcttgaccat tgcgcaaggc taattgaaat ttattgtatt cgggaagtgt ttccattata
     3121 accagatgtg ataaatgttt tcagctgtaa taagtgtacc gccgcatttt atatataaat
     3181 tatttgcagg agtgtttgct aattgtgttg ccacttgaac attttttaag ttgtggttag
     3241 cggcgatatc aagagcaaat tgaataagtt gagttgcaat ttgttgtttg cgatattttt
     3301 catctacggc aattaaacca atttctgcat aatcagaatg aaattgtaaa gtgataaaac
     3361 cttttattat tgtttcgttt ccgatataat aaataacatt tcccattgtg ccttgaaccg
     3421 attgtacaac ccattcagcg tacatttttt caaaaaatcc ttctggcatt ttagggtctg
     3481 ttttgaaacg ggaaaattca ccgcttgcaa tacctaagct gataagttgt tgatttgggg
     3541 ggagattcca actgcgcacg tgttcttgaa tttccggtaa taaattttga ttagccggaa
     3601 tttgatatac catttttgta tcacctaaaa ataagttttc tgttgtagcc aaacggttgt
     3661 tttcttcatt gtttgtcgga attttccaat ataacaaat
//
LOCUS       DAORKW010000369         3698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1318_length_3698_cov_5.074664, whole genome shotgun sequence.
ACCESSION   DAORKW010000369 DAORKW010000000
VERSION     DAORKW010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1318_length_3698_cov_5.074664"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..2473
                     /locus_tag="PLB46_12605"
     CDS             242..2473
                     /locus_tag="PLB46_12605"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRF77409.1"
                     /translation="MMRTAFKNFIEAFPVQLLVMHLKRNHFLIIIWIFLISIIAGLLG
                     TRYGVPLLFLDPEYFGRVNFLSFYIIGIALGGLFIVWNITSYILNAHRYSFLATLNRP
                     FGIYCLNNAILPAFFLIVYIFRIVGFQSSYGLLQLGAIAILIAGLISGIFTTIIISML
                     YFFRTNKDIFQIIGIINKKQQQTLQHNPDEQTHEEAADHHPISTSYYVSNKLQWKHAR
                     SADHYPESIIRSVYKQHHANALFIEITSLTIILLLGLLMENPVFRIPAGASIALLLSI
                     LIVLIGAYYYWMGAWKLFFFVVLFFVADRLMKSDKMNYENKIIGLDYTTPQHYSIDTL
                     KILSDSARTAADMQHTINMLENWRNKFPEKGPAPKLIVINCSGGGLRAMSFAMQVFQH
                     ADSVTQHQLMEHTPLITGASGGMLAAAYYRELYLQQKQGADIQIFDSQYADNMSADLL
                     NGLGFTMVVNDMFYPMQSFTSANTKHRKDRGYMFEQLLNENTGNVFDKPLSAYADAEY
                     AMEIPMIIFTPTIINDERKLYIATQPVSYLAQPVTRSTQYTAPEIDGVDVHQLFGTDA
                     NNMQFTSVLRMNCTFPYILPLVHLPTTPEIQVMDAGVRDNYGIQTSTQFIATFNEWIL
                     ANTSGVIVVNIRAIEQELGIKTNISNGVLQKFASPVESLYSNWVEIQDYQNDQLLNYL
                     NIILDGKLEVITFEYQPSENNKRASLSFHLTSLEKRDINNAVNNIYNKKAYDVLAREL
                     NKK"
     gene            2741..>3698
                     /locus_tag="PLB46_12610"
     CDS             2741..>3698
                     /locus_tag="PLB46_12610"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRF77410.1"
                     /translation="MKKALLFLLPIAMLSACSKTEMTIAEEQIGISDTEMKVKRYLTG
                     DPADVTTVTQPGFVLMGGSTDVDAAFEWMIERSGGGDFVVIRVTGSDGYNPYVYTDLG
                     GVNSIETMVIKGKKDGDDIGAYNTIINAEALFIAGGDQWDYVNLWKDTKVEDAINYLV
                     NVKHVPVGGTSAGLAILGDAYFDAKEGTVLSAEALSNPYNIKVSVQYNNFLDVPILTN
                     TITDSHYNSPDRKGRHITFMARLIQDYGIDARGIGVEEETAVCIDELGIGHVFGLNNA
                     YFLKKYGGNPETIVSGSPLTWDRGNQAIRVYKTPATAAGTNTF"
BASE COUNT         1182 a          634 c          686 g         1196 t
ORIGIN      
        1 atcaacaata gaaagtcaaa attatttaaa ttttctcaaa gattataaat attgtttaat
       61 ttatttgcaa atctcgaggt aaatctttta ttttgctatc aacctttaca ggttattggt
      121 taggcaacat acccctaaac ggatgctgca tgatgaggag catgcagcat ttccatttta
      181 tatcgtttcc actttttata tataaaaaaa ccttatttac gtttttacgt atagagggta
      241 tatgatgcga accgctttca aaaattttat tgaggcattc cccgtgcaat tgctggtgat
      301 gcatttaaag cggaatcatt ttcttattat catctggata tttttaatca gcattattgc
      361 aggtttatta ggcacacgat atggtgtccc cttattattt cttgaccctg aatattttgg
      421 ccgcgtaaat ttcttaagtt tctatattat cggtatcgca ctcggtggat tatttatcgt
      481 ttggaatatt accagctata ttttaaatgc gcatcgttat tcttttcttg caacgttaaa
      541 tcgcccattt gggatttatt gtttgaataa tgccattttg ccggcatttt ttttaattgt
      601 gtatattttt cggattgtgg gttttcaatc cagttacgga ttattacaac tcggcgcaat
      661 agccatatta attgccggtt taatcagcgg aatttttact acaattatta tttcgatgtt
      721 gtattttttc agaacaaaca aagatatttt tcaaataatc ggaattatta ataaaaaaca
      781 acaacaaacc ctgcaacaca accccgatga acaaacgcat gaagaggcag ctgatcacca
      841 ccctattagc acttcctatt acgtaagtaa caaattacaa tggaaacatg cgcgttcggc
      901 ggaccactac ccggagtcca taatccgctc ggtgtataaa caacatcatg ccaatgcgct
      961 gtttattgaa attacttcat taaccatcat tttattactt ggtttgttga tggaaaatcc
     1021 ggtattcaga attcctgccg gagcgagtat cgcattgcta ctttctatat taatcgtatt
     1081 aataggtgcc tattactatt ggatgggtgc atggaaatta ttttttttcg tggtattgtt
     1141 ttttgtcgcc gacaggttaa tgaaaagcga taaaatgaat tatgagaata aaataattgg
     1201 acttgattat acaacaccgc agcattattc tattgataca ttaaaaatat tatcggactc
     1261 agcacgcaca gcagccgaca tgcaacatac aataaatatg ttggagaact ggagaaataa
     1321 attccccgaa aaaggaccgg cgcctaaatt gattgttatc aactgcagtg gaggcggttt
     1381 gcgtgccatg tcgtttgcca tgcaggtgtt tcagcacgcc gatagtgtta cacaacatca
     1441 actaatggag catacgcctt taattaccgg agcctcaggt ggtatgttag ctgcagctta
     1501 ttaccgtgaa ttatatctcc aacaaaaaca aggtgctgat attcaaattt ttgattcaca
     1561 gtacgcagat aatatgagtg cagacctgct taatggtttg ggatttacta tggtggttaa
     1621 cgatatgttt tatcccatgc aatcatttac ttctgccaac accaaacatc gtaaagaccg
     1681 tggctatatg tttgagcaat tattaaatga aaataccgga aatgtatttg ataagccact
     1741 tagtgcatat gcggatgcag aatatgctat ggaaattcct atgattattt tcaccccaac
     1801 aataataaat gatgaacgta aattatacat tgcaactcaa ccagtaagtt atttagccca
     1861 acccgtaacc cgcagtacac aatatactgc acctgaaatt gatggcgtag atgtgcacca
     1921 attatttggt actgatgcca acaatatgca atttaccagc gtgctgcgca tgaattgcac
     1981 tttcccttat attttacctt tagtacatct tccaacaaca cctgaaattc aggtaatgga
     2041 tgcaggcgtt agagataatt atggcattca aacttccact caatttattg ctacatttaa
     2101 cgaatggatt ttagccaaca cgagcggtgt aattgtagta aatattcgtg ccatagaaca
     2161 agagttaggg ataaaaacaa atattagtaa tggtgtgtta caaaagtttg ccagtccggt
     2221 tgaaagttta tacagcaatt gggttgaaat tcaggattac caaaatgacc aactacttaa
     2281 ttatttaaat attattcttg atggcaaatt agaagtaatt acatttgaat atcagccttc
     2341 agaaaataat aaaagagcga gtttaagttt tcaccttact tcattggaaa aaagagatat
     2401 taataacgct gtaaataata tctacaataa aaaggcgtat gatgttttgg ctagggagct
     2461 caataagaaa tagacttgtg tattgacaat caatcagtta caaactacac aaaaccctgc
     2521 attttcagta attatttgaa caacagattg agtattatca tgcatttcaa ccggtttgcc
     2581 tcaatacaac tatttcacag ttatgtcaca ttttacaata aacagtaaac acataattaa
     2641 cctatgtata cactgttatt tagagtattc ggtatttttg aatgcgactt tttacaagcc
     2701 ttaaaaacaa accctattat ttaacccaat tttaaccatt atgaaaaagg ctttattgtt
     2761 tttattgccg atagccatgt tatcggcatg ttcaaagact gagatgacaa ttgctgagga
     2821 gcaaattggc atttccgaca ccgaaatgaa agttaaacgt tatttaaccg gtgaccctgc
     2881 agatgttacc actgttactc aacccggatt tgttttaatg ggaggaagta cagatgttga
     2941 tgctgcattt gaatggatga ttgaacgcag tggcgggggc gattttgtag ttattcgtgt
     3001 cactgggtct gatggttata atccttatgt atataccgat ttaggtggtg taaattccat
     3061 cgaaacaatg gttattaaag gcaaaaaaga tggtgatgat ataggtgctt ataataccat
     3121 tattaatgca gaggcattat ttattgccgg tggcgatcaa tgggattatg ttaatttgtg
     3181 gaaagacact aaggtagaag atgcaattaa ttacctggta aatgttaaac atgtacctgt
     3241 tggtggaaca agtgcaggtt tagccatatt gggagatgcc tattttgatg caaaagaagg
     3301 aacagtttta agcgccgaag cattaagcaa tccgtataat attaaagtta gcgttcaata
     3361 caataacttt ttagatgtgc caatattaac taataccatt actgattcac attataatag
     3421 tcccgacaga aaaggtcgtc atattacttt tatggcaaga ttgattcagg attatggaat
     3481 tgatgcacgc ggaattggtg ttgaagaaga aactgcagtg tgtattgatg agttaggtat
     3541 tggccatgta tttggattaa acaatgccta tttccttaaa aaatatggtg gcaatcctga
     3601 aacgattgta tcaggcagtc ctttaacatg ggatcgcggt aatcaggcaa tcagagtgta
     3661 taaaacacct gctactgcgg ccggaaccaa taccttta
//
LOCUS       DAORKW010000370         3690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1322_length_3690_cov_4.273177, whole genome shotgun sequence.
ACCESSION   DAORKW010000370 DAORKW010000000
VERSION     DAORKW010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1322_length_3690_cov_4.273177"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLB46_12615"
     CDS             complement(<1..351)
                     /locus_tag="PLB46_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRF77411.1"
                     /translation="MQKTPNSALARLSVEEFKNAEKTPFVVVLDNVRSLNNIGSVFRT
                     GDAFLLRGICLCGITAKPPHRDIEKTALGATESVYWEYFDHTIDAIHKLKNLGYAIVA
                     VEQASAAIMLQDFSP"
     gene            complement(419..934)
                     /locus_tag="PLB46_12620"
     CDS             complement(419..934)
                     /locus_tag="PLB46_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF77412.1"
                     /translation="MKDKQYKVFLVDDDTKHLIMLKNHLEKKSEYDIKVNIFSNGENC
                     LDKMHENPHIVILDYYLDGIKTDAANGLEILKKIRATNPDTYVIMMSGQDDLKVAIAT
                     IDNGAYDYIIKGESAYVRAQMIIDHIIENINNLEWKRNMKIRETALVTGFILLGCVLV
                     ALALYDMGMFD"
     gene            complement(931..2949)
                     /locus_tag="PLB46_12625"
     CDS             complement(931..2949)
                     /locus_tag="PLB46_12625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF77413.1"
                     /translation="MSEFDFQEQLRELLSKYYIHSKSIERELYKSELKYRSLLEQLTD
                     AIYFTSIDGHFLEFNRATVLLLGYSYDELKHLKVENTYANPEDRDNFKKIIAEDNEVI
                     DYELKLKKKDGELIDCLITSAIRHDAKGIPVGYQGIIRDITFRKKAEALQRAKDLAEQ
                     ANKFKAEFLANMSHEIRTPINAIVGMVHLLHQTPLSEKQTEYLAAIDTSAANLLQLIN
                     DILDFSKIEAGKLQLEEKYFSIKQVVQELINTIQFKAIEKNIGLIIDIDERVPAAVSG
                     DQLRLNQILLNLISNAIKFTQNGEIKVAVRLIDYVDEKARIYFSVKDTGIGISPEKLN
                     TIFESFTQANADTTRVYGGTGLGLAIVKKLIDMLQGAIMVKSKPGEGSEFIFEIDFKV
                     APEIVAQETAQKAGENDIPDVGSCKLLLADDHPINQLVTSELLKLTWPDMLVEIANDG
                     KEVLEKLQTSVYDIILMDVQMPEMNGLDATRAIRKMISPASEIPILAFTAYATTGEAE
                     KSISAGMDDYVSKPVSPQILTKKITDLLKKSGYQFPKEGAKRSDKPVNQQIDLNYFNI
                     MTGGDRDLQIKMMEIMMDETPAELALLQQHLKAQNWDGIRAVAHKMKSGMQYLGLTDV
                     LNLVKDIELSAKEKTNLELLPEKINTVVLSCEPALSQLKLAFEKIQQQ"
     gene            3019..>3690
                     /locus_tag="PLB46_12630"
     CDS             3019..>3690
                     /locus_tag="PLB46_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRF77414.1"
                     /translation="MLEAKQILQLVEAHIQQAGFPDEPKGLYDPAKYILSAGGKRIRP
                     CLTLMSANLFTDNIEPQIPVALAMEVFHNFTLVHDDIMDNAPVRRGLQTVHEKWNMHT
                     GILSGDAMLIQAYKLICQSPSSLLPKLLEMFNNTALLVCEGQQSDMDFEHQNEVTLDA
                     YIHMISNKTSVLLGCCMYCGAIAAGANEADAQHLYKTGLELGISFQIQDDVLDAYGDE
                     TTFGKK"
BASE COUNT         1063 a          749 c          624 g         1254 t
ORIGIN      
        1 gggagaaaaa tcttgcaaca taatagcagc tgaggcttgt tcaacagcta caatggcata
       61 acctagattt tttaatttat gaatggcatc tattgtgtgg tcaaagtatt cccaatacac
      121 actttcagtt gcacctagcg ctgttttttc aatatcgcgg tgaggtggtt ttgcggtaat
      181 gccacataaa cagattccgc gcaataaaaa cgcgtcgcct gtcctgaata cagagccaat
      241 attatttaaa cttctaacat tgtcgagaac aactacaaac ggtgtttttt cagcattttt
      301 aaattcctct acacttaatc tggctaaagc tgaatttggt gttttttgca tgtatcaatt
      361 tattaaaaaa cgaacaccat tatcaagaat tgtgttcact aatttattta acatttaatt
      421 aatcaaacat ccccatatcg tataacgcca aggcgacaag cacacagccc aataagataa
      481 aaccagtcac tagggcggtt tctcgaattt tcatgttgcg cttccattcc agattattga
      541 tattttcgat aatgtgatcg ataatcattt gagcacgcac atatgcactt tctcctttaa
      601 tgatataatc atatgcacca ttgtcgatgg tggcaatagc aacctttaaa tcgtcctgac
      661 cgctcatcat aatgacgtag gtatccggat tagtcgcgcg aatttttttc aaaatttcta
      721 acccgtttgc ggcatcagtt ttaatgccat ccaaataata atccaaaata acgatatgcg
      781 gattttcgtg cattttatca aggcaatttt ctccatttga aaaaatgttc accttgatat
      841 cgtattcaga ttttttttcc aggtggtttt tcaacatgat gagatgtttg gtatcatcat
      901 caaccagaaa tactttgtac tgcttgtctt tcattgttgt tggatttttt caaatgctag
      961 tttcaattgg ctcaacgccg gttcacacga caaaactact gtattaattt tttccggcaa
     1021 taactccaga ttggtttttt ctttggcact cagctcaata tccttaacca gattgagcac
     1081 atcggttaga ccaaggtact gcattccgga cttcatttta tgcgctacag cccttatccc
     1141 atcccagttt tgagccttga gatgttgttg caataaggca agctcggcgg gcgtttcatc
     1201 catcataatt tccatcatct ttatctgcaa atccctatct ccaccggtca taatattgaa
     1261 ataattcaaa tctatttgct gatttactgg tttatcagac cgttttgccc cttctttcgg
     1321 gaattggtat cccgactttt ttaataaatc ggtaattttt ttggtcagaa tttgaggtga
     1381 aaccggttta ctcacataat catccattcc ggctgaaata ctcttttcgg cttctccggt
     1441 ggttgcatag gctgtaaatg ccaatattgg aatttcggat gccggactaa tcattttgcg
     1501 aatggcacga gtagcgtcca atccattcat ttccggcatt tgcacatcca ttaaaataat
     1561 atcatataca gaggtctgca atttttccaa cacttcctta ccatcattcg caatttctac
     1621 caacatgtcc ggccacgtga gtttgagtaa ttcagatgta accagctggt taatcggatg
     1681 atcatctgct aataacagtt tacaactgcc cacatcagga atatcatttt ctccggcttt
     1741 ttgcgcggtt tcctgtgcga caatttccgg cgctactttg aaatctattt caaaaataaa
     1801 ctcactcccc tctcccggtt tacttttaac catgattgca ccctgcaaca tatcaatcag
     1861 cttcttcaca attgctaatc ccaaacctgt tccgccataa acccttgttg tatcagcatt
     1921 ggcttgggta aaactttcaa aaatggtatt gagtttttcg gggctaatac caattccggt
     1981 atcttttaca ctgaaataaa ttctggcttt ttcatctaca taatctatta aacgaacagc
     2041 cacttttatt tcgccgtttt gcgtgaattt gatggcattg ctgattaaat tcaacaatat
     2101 ttgatttaat cttaactggt ctcctgaaac tgcagcaggc actcgttcat caatatctat
     2161 aattaaaccg atattttttt caattgcttt aaactgtatg gtattaatta attcttgcac
     2221 cacctgttta atcgaaaaat atttttcttc caactgcaat ttaccggctt ctattttgga
     2281 gaaatcgaga atatcgttaa ttaattgcaa caaatttgcg gcagatgtat caatagcggc
     2341 taaatattct gtttgttttt cactaagtgg tgtttgatgt aataaatgca ccatacctac
     2401 aatagcatta attggtgtgc gaatttcgtg actcatattc gccagaaatt cagctttaaa
     2461 tttattggct tgttcagcta aatcttttgc gcgttgaagg gcctccgctt tttttctgaa
     2521 agtaatatct cgaataatac cctgatagcc gacaggtatg ccttttgcat cgtgtcttat
     2581 ggctgatgta attaagcagt caattaattc accatccttt ttcttgagtt ttaattcata
     2641 atctattact tcgttatctt cagcaattat ttttttgaag ttatcgcggt cttcaggatt
     2701 ggcataagta ttttcaactt ttagatgctt taattcatca taactataac ctaacaacaa
     2761 tactgttgct ctgttaaatt ctaaaaaatg accgtcaata ctggtaaaat aaatagcatc
     2821 ggtaagttgt tctaataagg agcggtattt aagttcactt ttatataatt cacgctcaat
     2881 gcttttacta tgtatatagt atttcgaaag caattcgcgc aattgttcct gaaaatcaaa
     2941 ttccgacatc gtttacaaat taacgcatta atcagcagat ttaaaacagt cattaatatt
     3001 cctcctatat ttgcagccat gttggaggca aaacaaattc ttcagttagt agaagcacat
     3061 attcagcaag cgggttttcc cgatgaacca aaaggacttt atgacccggc aaagtatatt
     3121 ttatctgccg gtggcaaacg tattcgtccc tgtttaactt taatgagtgc caatcttttt
     3181 accgacaata ttgaacctca aattccggtt gcgctggcta tggaagtgtt tcacaatttt
     3241 actttagtgc acgacgacat catggacaat gcgcctgtta gaagagggct tcaaactgta
     3301 cacgagaaat ggaatatgca caccggtatt ttatcgggag atgccatgtt aatccaagcg
     3361 tataaactga tatgtcaatc gccatcttca cttctaccta aacttttgga gatgtttaat
     3421 aataccgcat tattagtttg cgaaggtcag cagtctgaca tggatttcga acatcaaaat
     3481 gaagtgacac ttgatgctta tattcatatg attagcaaca aaacttctgt actgctagga
     3541 tgttgtatgt attgtggggc tattgccgca ggtgcaaatg aagccgatgc acaacatctt
     3601 tacaaaacag gccttgaatt gggtataagt tttcaaatac aggatgatgt tttagatgca
     3661 tatggtgatg aaaccacatt tggcaaaaaa
//
LOCUS       DAORKW010000371         3689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1323_length_3689_cov_3.771326, whole genome shotgun sequence.
ACCESSION   DAORKW010000371 DAORKW010000000
VERSION     DAORKW010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1323_length_3689_cov_3.771326"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1217
                     /locus_tag="PLB46_12635"
     CDS             297..1217
                     /locus_tag="PLB46_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77415.1"
                     /translation="MKTINYPNGFRKYFALLVCFIGVQIFSVNDLKAQGACDCKNIPK
                     SIDSAPTSCEVYCKLIKSFNRISHADSALINTIALESLDDYYKTFCMSKDTATGGFRF
                     YYGFYDDSIVQIICVANKEYDPDYELGYYVLPNSKKAGYNIDVNKVITKDSVIGLIKT
                     YIDTISIGKFDVDTIVPNDTVKYARYYQYAEIQKLIQEVTVDTGANKKIPAFITFEQC
                     YVPIKISTLYKTHPNIYYYGIPNDFNVGHSTVMYLHEKNTSDAPGDALTRNIPIAPNK
                     FPNNYQSGFLEIGKPCPPRCGEITWEDIIK"
     gene            1595..3604
                     /locus_tag="PLB46_12640"
     CDS             1595..3604
                     /locus_tag="PLB46_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77416.1"
                     /translation="MFAFYIWGYFIYFSIVLTIDKKQIIVKNSDSAAKLALLLPLGLS
                     FLPTLILGVVLIVKYEKFAGSILKIEGLLDIAIIILFVIIHLFGCIITYHKYYKNNDL
                     RNTLSRSSSLIFLFKRKKYKWWGFFSLLLLLSIAVIILLPEIVMRMMSEENILNPLSV
                     IGIGLSAYLVFFTLLGAYNNSLKKPLFILLLLYMALCSLTNNSHEIKTVSLSGEPTTG
                     DIRNTIEADFTEWIAAISEKDSSLNEIPVYIIAHQGGGIRGMSWSARVLQQLDQDIPG
                     FYEHIYAMTGASGGCNGMICYNTLKHFYPGEDATNLDPILDDILDYDYLTPTLATFIM
                     SEAVQSLSPLPISHFERSKSHANSFEQGFCQKGSRTMEIPTLSMYDGNNNLEHRIPHM
                     LINGTIAERGQKVIYSTLKLSDEFKADYDLVKYLNAEEGYPTRDLRVKDAMLVCCRFP
                     YILPGGTIPISVDSKRSDGNIVDGGYLENTGIIAALNLAFEIDSSYQKYIKKNVAECK
                     CQSTKVTPKGKCTPSREEIVCAHKKIKPIKINIIYVSNGNYQPDGSSAVSFAHETALP
                     IIGINSAREIAAEAANTETITTIIKWAGYEYGAFYLPRTKDEELALGWVMSDSGLAII
                     KNYATNLEHITIKNPNQYDNNIVNINTKTYEKIKGYHNNNSAKSK"
BASE COUNT         1261 a          564 c          652 g         1212 t
ORIGIN      
        1 aaaaatagca aaaaaacctg aaattcacaa tacaacaatg gatgcaaaca gcaactgcaa
       61 aagcatttgc aagtaaggct gtaactattc atattaattg gtttagctgc agtgcttata
      121 taattaggcc gaattccaac ataagtcttt aatgcttaac caatatccaa aagccgcaat
      181 aagttcattt gaacattatc aatcctactg ggtaaaaata acccttggga gggggtataa
      241 atacggtatt ttggttgaaa agcactattt acctttagtc tcaaaataat aaaaatatga
      301 aaacaattaa ttacccaaac ggatttagaa agtattttgc cttattggtt tgctttattg
      361 gtgttcaaat ttttagtgta aatgatttga aagcgcaggg ggcgtgtgat tgtaaaaata
      421 ttcccaaaag catagattct gcgccaacgt cttgtgaagt ttattgtaaa ctaataaaat
      481 cttttaacag aatttcacat gctgatagcg ccctaataaa taccattgcg ctcgaaagtc
      541 ttgatgacta ttataaaacg ttttgtatgt cgaaagatac tgcaacagga ggatttcgtt
      601 tttattatgg attttacgat gattcaattg tccaaattat ttgtgtagct aataaagaat
      661 acgatcctga ctatgaatta ggttactatg tattaccaaa cagtaaaaag gccgggtata
      721 acattgatgt caataaggtt attactaaag attcggttat aggtttaatt aaaacctata
      781 tagacacaat ttcaattggc aaatttgacg tggataccat agtgccaaat gatacagtga
      841 aatatgcacg atattatcaa tatgctgaaa tacaaaaatt aattcaggaa gtcactgtag
      901 atactggtgc aaataaaaaa atacctgcat ttataacgtt tgagcaatgt tatgttccga
      961 ttaaaataag tactttatat aaaacgcacc ctaatatata ttattatggt attcccaatg
     1021 actttaatgt aggtcattcc actgttatgt atttgcatga aaaaaacact tccgatgcgc
     1081 ccggggatgc gctgacaaga aatatcccaa ttgcacctaa taaatttcct aacaattatc
     1141 aaagtggatt tttggaaata ggcaaacctt gcccgcctcg ttgtggtgag attacgtggg
     1201 aggatattat taaataagat taaaaagtta ctgttattca aggtgtattg gaagtaagag
     1261 gtgacaattt gtttactact ttataccaaa tagcaaacaa ttaaatggtt gtaacgattg
     1321 cacaatggat tataataatt aatccaaatt cctaacttcc aatatttaat tacgataaaa
     1381 atagtattat atgcggtatt ataaaaaatc caaaaaggaa tccttgttca ctcagttcat
     1441 ggtatccggt tggccattaa tcagggttaa tttacattta atattgataa tcattattgg
     1501 aatttgcgct tttacaattc cggctcagtc acaggattat ttaaatcaat ttattgttaa
     1561 ttatgcaaag cacccatata atatattgaa tgttgtgttt gccttttata tatggggtta
     1621 ctttatttat ttttccatag tattaacaat tgataaaaaa caaattattg taaaaaatag
     1681 tgattcagct gctaaacttg cccttttact gcctttaggc ttatcgtttt taccaacact
     1741 aatacttggt gttgtgctta ttgttaaata tgaaaaattt gcaggttcaa tactaaaaat
     1801 tgaagggctg cttgatattg caattattat tttatttgta attattcatc tttttggttg
     1861 tataattact taccacaaat attataaaaa taacgacttg cggaatactt tatcaaggtc
     1921 ctcaagttta atatttttat ttaaacggaa aaaatataaa tggtgggggt tcttttcgtt
     1981 attgcttttg ctgtcaattg ctgttattat tttacttccc gagatagtaa tgcgtatgat
     2041 gtcagaagag aatattttaa atcctctctc tgtaattggc attggtttaa gtgcctattt
     2101 agtgtttttt acactgcttg gtgcgtataa taattcgctc aagaaaccct tatttatttt
     2161 attgctattg tatatggcat tatgtagtct caccaacaat agtcatgaaa ttaaaactgt
     2221 ttcattaagt ggtgaaccta ctaccggtga tattcgtaat actattgaag ccgattttac
     2281 agaatggatt gctgcaataa gtgaaaaaga tagttcttta aatgaaatac ctgtttacat
     2341 tatcgctcat cagggaggag gtatacgcgg gatgagttgg agcgccaggg tattgcaaca
     2401 gcttgaccag gatatccccg ggttttatga acatatttat gcaatgaccg gggcttcagg
     2461 cggatgtaat ggtatgattt gctacaatac attaaaacat ttttatcctg gggaggatgc
     2521 aacaaatttg gaccctattt tggatgacat tttagactat gattatttaa cacctacttt
     2581 ggcaacattt attatgtcgg aagcggttca atctttatca ccattaccta taagccattt
     2641 tgaaagaagt aaatcacatg ctaattcttt tgaacaagga ttttgccaaa aaggctctcg
     2701 cacaatggaa ataccaactt tatccatgta tgatggaaat aataatttgg agcatagaat
     2761 tccacatatg ctgattaacg gtacgatagc agaaagaggg caaaaagtta tctattccac
     2821 tttgaaatta tcagatgaat ttaaagctga ttatgattta gtaaaatacc tgaatgcgga
     2881 agaaggttat ccaactcggg atttaagggt aaaagatgct atgcttgttt gttgcaggtt
     2941 tccttatata ttaccagggg gaaccatacc gatttccgtt gatagcaaaa gaagtgatgg
     3001 taacattgta gacggtggat accttgagaa taccggcata atcgctgctt tgaatttagc
     3061 tttcgaaatt gattctagtt atcagaagta tataaaaaaa aatgtagctg agtgcaaatg
     3121 tcaaagtact aaagttacgc ctaaaggtaa atgtactccc tcccgcgaag agattgtgtg
     3181 tgcgcataaa aaaattaaac ccattaaaat taacatcata tatgtatcaa acggcaacta
     3241 ccaacctgat ggtagttctg ctgttagttt tgcgcacgaa acagcgttac caattattgg
     3301 tattaacagt gccagagaaa ttgcagcgga ggctgcaaat acggaaacta taaccactat
     3361 tattaaatgg gcgggttacg aatacggagc tttttatttg ccaagaacca aagatgagga
     3421 attagcttta ggatgggtga tgtcagatag tggactggca ataattaaaa attacgcaac
     3481 aaaccttgag catattacta taaaaaaccc caatcaatat gataataata ttgtaaatat
     3541 aaataccaaa acctatgaaa aaattaaagg atatcacaat aacaattctg caaagagtaa
     3601 gtaaagactt cgaacgtttt ttttattgga tttgtcgaca taatttccca ttaattctgc
     3661 ttgcatatgt tgtattggca ctttaccct
//
LOCUS       DAORKW010000372         3681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1326_length_3681_cov_5.385825, whole genome shotgun sequence.
ACCESSION   DAORKW010000372 DAORKW010000000
VERSION     DAORKW010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1326_length_3681_cov_5.385825"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLB46_12645"
     CDS             complement(<1..451)
                     /locus_tag="PLB46_12645"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRF77417.1"
                     /translation="MQIIKLRNQLTPISNQLKAEGAKIGFVPTMGALHQGHISLVEAS
                     KKRANITIVSIFVNPTQFNDPKDLEKYPRPIEQDIRMLEAAGCDILYLPEASDIYGPV
                     TQVTDKFDLAGLDLELEGASRPGHFAGVAQVVKILLEITRPDILFLGQ"
     gene            633..1454
                     /locus_tag="PLB46_12650"
     CDS             633..1454
                     /locus_tag="PLB46_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRF77418.1"
                     /translation="MDKKRVLIITQEMQPYLNGTDIGDIARELPQYVQDKGNEIRVLM
                     PRYGVINERRHRLHEVVRLSGMNIILNDDDYPLIIKVASVPGARMQVYFLDNDDLFKR
                     KYVFHDENNNFYEDNAERMVFFCKAALETVKKFQWAPDIIHCHGWMTSLIPLYIKTAY
                     KNEPVFQNSKVVYSMYNGAFEGNLSKEKFLEKAPISAMVEPEHLNAYKEYNHLGLSKG
                     AATYADGVIIGSGEIDKDISKFLKSQGKPVLEFTQEEYLDKYAEFYDNLAPIISK"
     gene            1471..2826
                     /locus_tag="PLB46_12655"
     CDS             1471..2826
                     /locus_tag="PLB46_12655"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HRF77419.1"
                     /translation="MNKAVFLLIGGLIVLTASCRKDNFIGEELLPEEDFLNSARIDTF
                     TMITYTDIDDSIVTSQNPFYALGSMQSDIYGKSTAGIFAQLLLPANNLFFGDNATVDS
                     VVLTLDYASYYGDTSATHCVSVYRMTEPLYNNRNYYSNQKFHYMPIPIGKKNEFKANN
                     RDSVVLADGSEWEPHLRINLFDAFGQNIVNLDSAILENDTAFLQYLQGLYIAADTLTD
                     GYSRGMMYFDMSSDITGVRIYYKNSEADSLSVLFPFDGVKTNYFKNTYPEASQAYNAI
                     VNPDTTNGEAFTYVKGFTAMRTMIKLPTLTNLKDVSVNKAELIFTATTDDGRFFNAPP
                     KLILLPQDSTGHNSYYFALYSEEYFSSIVDDNFGGTDIGGTIVETVSEETGRTLYQYK
                     FIITRHVQEMLEGSIDNYGFALSCIPGNRIPNAVTLGGINSPRASFKPYLSITYTTIN
                     K"
     gene            2831..>3681
                     /locus_tag="PLB46_12660"
     CDS             2831..>3681
                     /locus_tag="PLB46_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRF77420.1"
                     /translation="MCGIVGYIGHRQAYPVIIKGLHRLEYRGYDSAGVALINGDLRVY
                     KIAGKVSELEKETEDKNVEGTCGIGHTRWATHGEPNTKNAHPHVSESKNLAIIHNGII
                     ENYASLKTELIKRGHQFLSDTDTEVLIHLIEDIQLNEKTDLVEAVRIALNQVIGAYAI
                     VIASKDEPDMLIAARKSSPMVVGIGEGEFFIASDASPLIEYTRNVVYLNDEEIAVVRR
                     NQELVIKTIHNETITPYMQKLQMQLDMIEKGGYDHYMLKEIYEQPNSIMDSMRGRLIG
                     SKNAIVLG"
BASE COUNT         1191 a          647 c          745 g         1098 t
ORIGIN      
        1 tttgtccgag aaacaaaata tcggggcggg taatctccaa taatatcttc accacctggg
       61 ctacaccggc gaaatgtccc ggccggcttg caccttctaa ttcaaggtcg agaccggcga
      121 gatcaaattt atcggtcact tgggtaaccg gtccatatat gtcgctggct tcgggtaggt
      181 ataaaatgtc gcaacccgcg gcttccagca tgcggatatc ctgttcaatt ggtcgcggat
      241 atttttctaa atctttgggg tcattgaact gggtcgggtt cacaaaaatg ctcacaatcg
      301 taatattcgc gcgcttttta ctggcttcca ccagcgaaat atgcccctga tgaagggctc
      361 ccatggtggg aacaaagcct attttggccc cttcagcctt caattggttg ctaatagggg
      421 tgagttggtt gcgtagtttt attatctgca tgcttagtgt tataagggtt ttcggtcgtt
      481 ttaaggttac tttaacaaag tccaaaatcg agttgcataa gtaaattcta aactatattt
      541 tgtaattttg cagcttcaaa attaaacttc tgagcagact taggaagtat acacttcgca
      601 aaataaccta acagtcaagt aacaagtgaa atatggacaa gaaaagagtg ttgattatca
      661 cccaggaaat gcagccttac ctaaacggga cagatattgg ggatatcgca agagaactac
      721 cacaatacgt tcaagataag ggaaatgaga ttagggtttt gatgcctcgt tacggggtga
      781 taaatgaacg tcgtcacaga ttacatgaag ttgtgcgttt aagtggtatg aatatcattt
      841 tgaacgatga cgactatccg ctgataatca aagtggcttc agtgccgggt gcaagaatgc
      901 aggtgtattt tctcgataac gacgatttgt ttaaacgcaa atacgtgttc cacgatgaaa
      961 acaataattt ttatgaggat aacgccgaac gtatggtgtt tttctgtaaa gcagcacttg
     1021 aaacagtaaa aaaattccaa tgggcaccgg atattatcca ttgccatggt tggatgacaa
     1081 gtttgatacc gttatacatt aaaacagctt ataaaaacga accggtattc cagaactcaa
     1141 aagtggttta ctctatgtat aatggtgctt ttgaaggtaa cctttctaaa gaaaaatttt
     1201 tagaaaaagc accaatcagc gctatggttg aacctgagca tcttaatgcg tataaagaat
     1261 ataaccacct tggccttagc aaaggcgcag caacttatgc cgacggtgtt attataggca
     1321 gcggagaaat tgacaaggat atcagcaagt ttctcaaatc gcagggtaaa cctgtgcttg
     1381 aatttacaca ggaagaatac ctggataaat acgctgaatt ttatgacaat ttagctccaa
     1441 taatttcaaa ataaatccgt tataggcagg atgaacaagg cagtgtttct attgattggc
     1501 gggttgattg tattaacagc atcgtgccgg aaagataatt ttataggtga agaactactt
     1561 ccggaagaag atttcttaaa ctcagcgagg atcgatacgt ttacgatgat aacctatacg
     1621 gatatcgacg attctattgt aacaagtcaa aatccttttt atgcacttgg cagcatgcaa
     1681 agtgatattt acggaaaatc tacagccggt attttcgcac aacttttatt gccggcaaac
     1741 aatttgtttt ttggcgataa tgcaacagtt gactcagtag ttttaacttt agattatgcg
     1801 agttattatg gtgatacttc agcaacacat tgtgtgtctg tttaccgtat gacagagcca
     1861 ttgtataata acagaaatta ttattccaat caaaaatttc attacatgcc aattccaatt
     1921 ggtaaaaaga atgaatttaa agcaaataat cgcgacagtg ttgttttagc agatggttca
     1981 gaatgggaac cacatttacg cattaattta tttgatgcat tcggacaaaa tattgtaaat
     2041 cttgattctg ctattttaga aaacgatact gctttcttac aatatttaca aggcttatat
     2101 attgccgctg atacattaac cgatggttat tcacgcggga tgatgtattt cgatatgtcg
     2161 agtgatataa ccggtgttag aatttattat aaaaatagtg aagccgattc gttaagcgta
     2221 ttatttccgt ttgatggtgt taaaacaaat tattttaaaa acacctatcc ggaagcttca
     2281 caagcatata atgcaattgt aaatccggat actaccaatg gtgaagcatt tacttatgta
     2341 aaaggattta ccgctatgcg caccatgatt aaactgccaa cacttactaa tttaaaagat
     2401 gtttctgtaa acaaagcaga attaattttt acggcaacta ccgatgatgg tcgttttttt
     2461 aatgcgcctc ctaaattaat tttattacca caggattcta ccggacataa ttcctattat
     2521 ttcgcattat acagtgaaga atatttttct tctatagtag atgacaattt cggcggaacg
     2581 gatattggtg gtacaattgt ggaaactgtt tcggaagaaa ccggcagaac attatatcaa
     2641 tataaattta tcattacacg acatgtgcag gaaatgctgg aggggtcaat cgacaactac
     2701 ggatttgctt tatcatgtat tcccggaaat cgtatcccta atgcagtaac tttaggtgga
     2761 attaattcac caagagcgag ctttaaacct tatctctcga ttacatacac aacgataaac
     2821 aaataacatc atgtgtggta tagttggtta cattgggcat cgtcaggctt acccggttat
     2881 cattaagggc ctgcatagat tagaatatcg cggttacgac agtgccgggg tagcattaat
     2941 taacggcgac ttgcgcgttt ataaaattgc aggcaaagtt tctgaactcg aaaaagaaac
     3001 tgaagacaaa aatgtagaag gcacttgtgg catcggtcat acccgatggg caacacatgg
     3061 tgagccaaat acaaaaaatg cacacccaca cgtaagtgaa agtaaaaatt tagccattat
     3121 tcacaatggt attatagaaa attacgcttc cctaaaaaca gaattaataa aaagaggtca
     3181 tcagttttta tctgataccg atacagaagt tttaatccat ttaattgaag atattcagct
     3241 caacgaaaaa acagatttag ttgaagctgt tcgtattgca ttaaatcagg taattggcgc
     3301 ttacgcaatt gtaattgcat cgaaagatga acctgatatg ttaattgcgg cgcgcaaatc
     3361 ttctcctatg gttgttggta ttggagaagg agaatttttt attgcttctg atgcatctcc
     3421 attaattgaa tatacgcgca acgtagtgta tttaaatgat gaagaaattg cagttgttcg
     3481 tcgcaatcag gagttggtaa ttaaaacaat tcacaacgaa acaattacac cttacatgca
     3541 aaaattgcaa atgcaattag acatgattga aaaaggtggt tacgatcatt acatgctcaa
     3601 agaaatttat gagcaaccta attccataat ggacagtatg cgcggtcgtt taattggcag
     3661 caaaaacgca attgttttag g
//
LOCUS       DAORKW010000373         3678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1329_length_3678_cov_4.522219, whole genome shotgun sequence.
ACCESSION   DAORKW010000373 DAORKW010000000
VERSION     DAORKW010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1329_length_3678_cov_4.522219"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..709)
                     /gene="hscB"
                     /locus_tag="PLB46_12665"
     CDS             complement(188..709)
                     /gene="hscB"
                     /locus_tag="PLB46_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HRF77421.1"
                     /translation="MYYFELYQIPVSFLPDIASIKKQYFVLSKQYHPDFYSNTSPEKQ
                     AEILALSTQVNKAYAVLTDMDKRMRYILMEAGYLQEEEKFILPQDFLMEMMDINEKLM
                     EVQMDGDAVATAKIGNEVDVLESNLFEQIKPVLTSFTETSDAQIYQEIKTYYFKRKYL
                     LRIKSQIAGTVEM"
     gene            complement(715..1548)
                     /locus_tag="PLB46_12670"
     CDS             complement(715..1548)
                     /locus_tag="PLB46_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77422.1"
                     /translation="MNRFFSVSCCLILCFAFTIVNGQNANSGALLSQDSNKHKVMIIP
                     FDQYNYFSDADPELAEKNEKPVTDISRLFRYGLNYNISARVISTNDAYNILTDTTVQS
                     GADLKMIYASITYRFEHPMNVVKGDSVGKAVAQPDLFGNGVVEEEQKEKSNPFKKKSE
                     DEAEKNAKYLNAVITHPEIFTGLQQKYGTDLFLFINQFELVTNYNHCLDRSANYFERK
                     VVVHYSIYDANAKQLQGGAHTVTFSSGETNVDKIIATQFPLVADYLSTAIADVSKPVL
                     K"
     gene            complement(1548..2867)
                     /gene="rseP"
                     /locus_tag="PLB46_12675"
     CDS             complement(1548..2867)
                     /gene="rseP"
                     /locus_tag="PLB46_12675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRF77423.1"
                     /translation="MDVVIQIAQLILALTILVFIHELGHFMAARMFGIRVEKFFIFFD
                     AYGKKLWSIKRGDTEYGIGWLPLGGYVKIAGMIDESMDKDAMKKPAEPWELRSKPNWQ
                     KFIVMIGGIVMNVILGIILYVFILANYSKNYTTVEGVNNAGGIYATEAGKKYGFETGD
                     KLISINGRSYERLDDYVSARLYFGSDVVVDRNGKQVELVVADSAFKDKMRGDVLFDLK
                     ATIKIEDFSDSLQNAKEAGLQKGDILKAIDSVPVEYFTQMSEMFKARKGQSVPITVDR
                     NGTEQTVLTQVDEHGRIGILTNTKLYAYDSVHYSFGKALSYGTRDAFEVFYFQAVSLV
                     RIFRGDIPARESISSPIGITKLFAPKWDWTSFWILTALLSMVLAFMNLLPIPALDGGH
                     IMFIAIESITGRKLSDKFMERAQLVGMILLLSLMVFAVGNDIIKMFN"
     gene            complement(2927..>3678)
                     /locus_tag="PLB46_12680"
     CDS             complement(2927..>3678)
                     /locus_tag="PLB46_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRF77424.1"
                     /translation="YEPYRSKIEAIVGKKIPSIETYPASEGFIAYQDSQNADGLLLNV
                     DSGIFFEFIPAGEIFNDNPTRVMLKDVQLGVNYALILNNNAGLWGYNIGDTIKFVSLN
                     PYRIVVSGRIKHFISAFGEHVIGEEVEYALLKAAEEEQIEIMEFTVAPQVNPESGLPY
                     HEWFIEFGTQPANLTTFAAKVDEHLQHKNIYYKDLITGKILRSLEITLVKEGGFRNFM
                     KAKGKLGGQNKVARLSNDRLIAHELKEWTVC"
BASE COUNT         1124 a          776 c          569 g         1209 t
ORIGIN      
        1 cacacgcttt ttgagggcac cggattttga gtccggcgtg tctaccattc caccatttcg
       61 gcatgtatac tcatttttca aaaatgaggt ggagctgatt aataatcaat cagggttgca
      121 aagttaatcg aatagcgctt attttcaaat aaaaattata ttaaacatag gattggcatc
      181 ctattcctta catttcaacg gttccagcaa tttgcgattt aatccgtaag aggtattttc
      241 tcttaaagta atatgtcttt atttcctggt aaatctgcgc atccgatgtt tccgtaaacg
      301 acgttaatac tggtttaatt tgctcaaata ggttactctc cagaacatca acttcgttcc
      361 ctattttcgc agtggcgaca gcatcaccat ccatttgcac ttccatcaac ttttcgttga
      421 tgtccatcat ttccatcaaa aaatcctgtg gtaatataaa tttttcttct tcctgtaaat
      481 acccggcttc cattaaaata tatcgcatgc gtttatccat atcggttaaa acggcatagg
      541 ctttattcac ctgggtagat aacgctaaaa tttcagcttg tttctccgga gatgtattac
      601 tgtaaaaatc aggatgatat tgtttactta atacgaagta ttgcttttta atgctggcga
      661 tgtccggtaa aaaactgacc ggtatttgat ataattcaaa ataatacatg gtatttattt
      721 tagtaccggt ttagatacat cagctattgc tgtgcttaaa taatcggcaa ctagcggaaa
      781 ctgtgtagct attattttat ctacattcgt ctctcccgaa gaaaacgtaa ctgtatgcgc
      841 gccgccttgt aattgtttgg cattagcatc atatatggaa taatgtacca ctacctttct
      901 ttcaaaataa ttggcagaac gatccagaca atgattatag ttggtcacca gttcaaactg
      961 attaataaat aaaaacaaat cggtcccgta cttttgctgc aagcctgtga aaatttccgg
     1021 atgagtaata acagcattca aatacttggc atttttttca gcttcatctt cagatttttt
     1081 cttaaaagga ttactttttt ccttttgctc ttcttccaca acaccattgc caaacaaatc
     1141 gggttgcgca acagccttac ctaccgaatc gccctttact acattcatcg gatgttcaaa
     1201 acgatacgta atactggcat aaatcatttt taaatctgca cctgattgca cagtggtatc
     1261 ggtcaaaata ttataggcat cattagtaga aattaccctt gcactaatat tataattaag
     1321 tccgtaacga aacaatcgag aaatgtccgt aaccggcttt tcattttttt ctgctagttc
     1381 agggtcagca tcagaaaaat aattatactg gtcgaaaggt ataatcatta ccttatgttt
     1441 atttgaatcc tgactgagta gcgcgccgct gttagcattt tgtccattta caatggtaaa
     1501 tgcaaaacac agtattaaac aacagctaac actaaaaaat ctattcatca attaaacatt
     1561 ttgataatat cgttgcctac agcaaatacc atcagcgata acaataaaat catgccgact
     1621 aattgtgcac gttccataaa tttatcggac aattttctgc cggtaatact ttcaatggcg
     1681 ataaacatta tatgtccacc atctaatgcc ggaattggta acaaattcat aaacgccaac
     1741 accatactca ataatgcagt taaaatccaa aatgaagtcc agtcccattt tggcgcaaat
     1801 aatttggtaa taccaatagg gctcgaaata ctttcacgtg caggaatatc acctctgaaa
     1861 atgcgcacca gactcactgc ttggaaataa aatacttcaa aagcatcgcg ggtaccataa
     1921 ctcaaggctt tgccaaaact ataatgcacc gaatcgtaag catacaattt ggtattggtt
     1981 aaaataccta ttctgccatg ttcatccact tgcgttaata ctgtttgttc agtgccattt
     2041 ctgtctaccg taattggaac actttgtcct tttcttgctt taaacatttc ggacatttgt
     2101 gtaaaatatt ctaccggaac actatcaatt gcttttaaaa tatccccttt ttgtaatccg
     2161 gcttctttgg cattttgtaa gctatcgcta aaatcttcaa ttttaattgt tgcttttaaa
     2221 tcgaacaata catcgccacg cattttatct ttaaatgcac tatccgcaac tactaattca
     2281 acttgtttac cattgcggtc aaccactaca tctgaaccaa aatataatct tgcagataca
     2341 tagtcatcta aacgctcata acttctgcca ttaatagaaa ttaatttatc gccggtttca
     2401 aaaccatatt tttttcctgc ttcagtagca taaataccac ctgcattatt tactccttca
     2461 acagtagtat aatttttaga ataattggct aaaatgaaca catataaaat gatacccaat
     2521 atcacattca taacaatacc gccaatcatt acaataaatt tctgccaatt gggtttagaa
     2581 cgtaattccc aaggttctgc cggttttttc atggcatctt tatccatact ctcatcaatc
     2641 ataccggcaa tttttacata accacctaaa ggcagccagc caatgccata ttcagtatcg
     2701 ccgcgtttaa tcgaccataa ttttttccca tatgcatcaa aaaatatgaa gaatttttct
     2761 actcttatcc caaacatcct tgcggccata aaatgcccca gctcgtgaat aaaaaccaaa
     2821 attgtaagcg ctaaaatcaa ttgcgcaatc tgtataacta cgtccataag tgctgcgaaa
     2881 ttcgtgaatt atttgttgat tgtgttcatg ggtcaattaa actttgttaa cacactgtcc
     2941 attcttttag ttcatgggct atcagcctgt cgttactcag tctggcgact ttgttttgcc
     3001 cgccaagttt gcctttagcc ttcataaagt tgcgaaaacc gccttctttt accaacgtaa
     3061 tttcgaggga tcgcagtatt ttgccggtaa tcaggtcttt ataatagata ttcttatgtt
     3121 gaaggtgttc atctactttg gctgcaaagg ttgttaagtt agccggttgg gtgccaaatt
     3181 caataaacca ttcgtgataa ggcaatccgc tttcaggatt aacctgaggt gctacggtaa
     3241 attccataat ttcaatctgt tcctcttctg ctgcttttaa taaggcatat tccacctctt
     3301 ctcctatcac atgttcgcca aatgcagaaa tgaaatgttt aatacgaccg gaaactacaa
     3361 ttcgatatgg gtttagggaa acaaatttta tagtatcacc aatattatat ccccacaaac
     3421 ctgcattatt gtttaagata agtgcatagt ttacgcctaa ttgaacatcc ttcaacataa
     3481 cccttgttgg attatcatta aatatttccc cggccggaat aaattcgaaa aatatccctg
     3541 aatctacatt gagtaaaagg ccgtcagcgt tttgactatc ctgataagca ataaatcctt
     3601 cgctggcagg atatgtttca atggacggaa tttttttacc gacaattgcc tcaatcttag
     3661 agcgatacgg ttcgtaat
//
LOCUS       DAORKW010000374         3675 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1331_length_3675_cov_4.631215, whole genome shotgun sequence.
ACCESSION   DAORKW010000374 DAORKW010000000
VERSION     DAORKW010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1331_length_3675_cov_4.631215"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLB46_12685"
     CDS             complement(<1..246)
                     /locus_tag="PLB46_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616740.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRF77425.1"
                     /translation="MYDNNPTLEGLALNKPLEEFRQGEALGLENMEQTATGKKFYIES
                     YGCQMNFSDSEIVASILFESGFGLTKNFEEADLILLNT"
     gene            313..1788
                     /locus_tag="PLB46_12690"
     CDS             313..1788
                     /locus_tag="PLB46_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77426.1"
                     /translation="MKINEQKDRFSLKQLLTSPETVIRAAILFICMFNASLLGLPFFG
                     DHVTLISIPASDYYNIGFQSIWLPEGHATGHPPFYALLTALSWTVFGKSLLISHLLTL
                     IFSLVLIAQYARLTKQMAEPRMRSLALILLLANPVFLAQMAAMSIDVFLTAVFLWGIN
                     VIHSRKKLLLIVACILLTISSLRGFMLVVILFLYDWVYHKYTFKQLTINFLYYIIACI
                     PVTLYFIFQYRHSGWWLVPTDGNWSEGRQFSDKTNILPKLFEFGLRFVEFGMLIPMVV
                     FIYYLIKNYQTNRFNHKFVLILIGISVFAVFIIPFQNSVLIRYLLPLQLLVVLLFSEM
                     VMHFKHVFLRKIAIGLTLIMMIGQHFVVYPETKSSAWAYNWGDGSLAHLSYFTFRTAC
                     HDYVVANKIPDDSIYAGFPDYKPFEFTDLNNDTIAYNSSDLDCSKYIILSNVMNNIPY
                     WEKEQIRTHAKEVKRWSRYPVEYVLYENTNLHNKYNDEVCK"
     gene            1889..3502
                     /locus_tag="PLB46_12695"
     CDS             1889..3502
                     /locus_tag="PLB46_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRF77427.1"
                     /translation="MESIIPIGYWIFFIITIVGTFVGLYKLFEKAGYAGWKALVPVYN
                     VWICTKITGKGLKWFILLMIPVLNVVVWLLMANELSKVFGKDGFWAYVGSMLVPYFYF
                     PKLAFDKDVKYIGPHESPKKSTGREWADALAFAVVAATLIRTFFFEAYTIPTTSMEST
                     LKAGDFLFVSKFHYGARFPITPLAFPFAHHTMPVIKTKAYSDAIQLPYMRFPGIEKIH
                     RNSSVVFNFPEGDTVYLPNQQNSYYSLLMNDAEMMRAYDKKNNNTEKPFSYYYNQMRS
                     RYEASGELAYRPVDKRDNYIKRCVGLPGDSLAVVDGILYVNGKKAFVAEHQQKSYDVK
                     FKENVNATNAGNILLDLSLNPMDFQDTVMMGKNIVITANMNISNAATLEKNPSVESVS
                     LNHYDRLRYGYIGLFPHNPKGMPNSVDDFSQIWIPKKGATIAITPQNYYNYQRIIEVY
                     EHNTVTKDNRGNIFMDGKPLTQYTFKQNYYWMMGDNRHRSYDSRYWGYVPEDHIVGKP
                     VLVWFSWNKNLPLLKRIGSIRLDRMFNFI"
BASE COUNT         1150 a          650 c          654 g         1221 t
ORIGIN      
        1 ggtgtttaac agaattaaat ctgcttcttc aaaattttta gtcagcccaa acccactttc
       61 aaataagata ctagccacaa tttcgctgtc gctgaaattc atttggcagc cataactttc
      121 aatataaaat ttctttccgg ttgcggtctg ctccatattt tccaatccca aagcttcccc
      181 ctgacggaac tcttcgagtg gtttatttag cgctaatcct tccaatgttg gattattatc
      241 gtacatttct ccttaagttt gatgcaaaat tacttaatat actgtcaact tgtcactatt
      301 tcaccctaca acttgaaaat taacgagcaa aaagaccgat tttcactcaa gcaattactt
      361 acctctccgg aaacagtaat tcgggcggct attttgttca tttgtatgtt taatgccagt
      421 ttgctgggtt tacccttctt tggggaccat gtgacgttga tcagtatccc agcgagtgac
      481 tactataaca tcggctttca atccatttgg ttgcctgaag gacatgctac aggccatcct
      541 ccattttatg ccttattaac cgcattaagt tggacagttt ttggcaaaag tttactgatt
      601 tcacatctgc taaccctcat tttttcttta gtattaattg cccaatatgc gcggctgact
      661 aaacaaatgg cagaacctcg catgcgttca ttagccctca tattactatt ggctaacccg
      721 gtatttctcg cccaaatggc tgctatgagc attgatgtgt ttttgacagc tgtgttttta
      781 tggggaataa atgttattca tagcaggaaa aaattactgc taatagttgc ttgtatcttg
      841 ctgactataa gcagtttacg tggatttatg ctggtggtaa ttttgttttt atacgattgg
      901 gtttatcaca aatacacatt taaacaactg acaatcaatt ttttatatta catcattgca
      961 tgtattcctg taactctata ttttatcttt caataccgac attccggatg gtggttggtg
     1021 ccaaccgatg gaaactggag tgaagggcgc caattttccg ataaaacgaa catcctccca
     1081 aaactctttg aatttgggct ccgctttgtg gaatttggga tgttaattcc aatggttgta
     1141 tttatttact atttgataaa aaactatcaa accaatcgtt tcaaccacaa gtttgtgctc
     1201 attctaattg gtattagcgt ttttgcagtg tttatcatac catttcaaaa ttcggtatta
     1261 atccgctacc tgctgccatt acaattgctt gtagtgttat tattcagtga aatggttatg
     1321 cattttaaac atgttttctt gcgtaaaata gcaatcggat taaccctgat tatgatgata
     1381 ggccaacatt ttgttgttta tcccgaaaca aaaagcagtg cctgggctta taactggggt
     1441 gatggctcgt tggcacatct ctcctatttt acgttccgaa ctgcttgtca cgactatgtt
     1501 gtagccaata aaatacctga tgatagtatt tatgccggat ttccggatta caaaccgttt
     1561 gaatttacag atctaaacaa tgacacgatt gcctataata gcagtgattt agactgcagc
     1621 aagtatatta ttctgagtaa tgtgatgaat aatattccat attgggaaaa ggagcaaatc
     1681 cgcacacatg caaaagaggt aaaacgttgg tctcgctatc cggttgagta tgtgttatat
     1741 gaaaacacca atttgcacaa caaatacaat gatgaggttt gtaaatgaca gttcacaaca
     1801 atgctgttaa tcgaataaaa gccactattc gccgaaaaca tatagttttg tgcccgttag
     1861 attgatttct ttacataaaa aattataaat ggaaagtata attcctattg gatattggat
     1921 attctttatt attaccatag taggcacatt tgtcggttta tataaattat ttgaaaaggc
     1981 cggatatgca ggatggaaag cacttgttcc cgtttataat gtctggattt gcacaaaaat
     2041 tacaggaaaa ggccttaaat ggtttatcct tttaatgatt ccggtattaa acgttgtggt
     2101 ttggttactc atggcaaatg agttatcgaa agtttttggg aaagatggat tttgggctta
     2161 tgtaggttct atgttggttc cttatttcta ttttccgaaa ctggcttttg acaaagatgt
     2221 taaatatata ggtcctcacg aatcaccaaa aaaatcgaca gggagagaat gggcagatgc
     2281 attagcattt gcagttgttg cggcaacatt aatcagaact ttcttttttg aagcatatac
     2341 catcccaact acttcaatgg aaagtacatt aaaagcaggt gatttcttat ttgtaagtaa
     2401 attccattat ggtgcaagat ttccaataac acctttagca tttccatttg cacatcatac
     2461 catgccggta attaaaacaa aagcatattc agatgctatt caattaccat acatgcgttt
     2521 tccgggtata gaaaaaattc accgtaacag ttcagtagta tttaattttc ctgaaggtga
     2581 tacggtttat ttgcctaatc aacaaaacag ttattattcg ttgcttatga atgatgctga
     2641 aatgatgcgt gcttatgaca aaaaaaataa taataccgaa aaaccattta gttactacta
     2701 taatcaaatg cgttcaaggt atgaagccag tggtgaatta gcatatcgtc ctgttgataa
     2761 acgcgataat tatattaaac gttgtgttgg attacccggc gattcattag cggttgtaga
     2821 tggcatttta tatgtgaatg gtaaaaaagc atttgtggca gaacatcaac aaaaatcgta
     2881 tgacgtaaaa tttaaagaaa atgttaatgc taccaatgca ggaaatattt tattagattt
     2941 atcgttaaac ccaatggatt ttcaggatac ggtaatgatg ggtaaaaaca tagtgattac
     3001 ggccaacatg aatatttcaa atgctgcaac tttggaaaaa aatccatcgg ttgaaagtgt
     3061 atcattaaat cactatgaca gattgcgtta cggatatatc ggattattcc cgcataatcc
     3121 gaaaggaatg ccaaattcag ttgatgattt ttcgcaaatt tggattccta aaaaaggtgc
     3181 aacaattgcc ataactccac aaaattatta taactaccag cgtattattg aagtatatga
     3241 acacaacacc gtaactaaag ataacagagg taatattttt atggatggca aaccgttaac
     3301 acaatacaca ttcaaacaaa attattattg gatgatgggt gataatcgcc atcgctctta
     3361 cgattcacgt tactggggct atgtacctga agaccatatt gtaggtaaac ctgttttagt
     3421 ttggtttagt tggaataaaa atcttccttt acttaaacgt attggttcta ttcgtttaga
     3481 cagaatgttt aattttatat aaatgtataa tcgttcacca tatattatgt tattgttttt
     3541 actgcttgtt gcggcaagtt gtaaaaacaa taccggtgaa acaaacgaaa aaccgcaaac
     3601 aataaccgat accacttctg aattagataa aattatagaa tcggaagcta ctactccacc
     3661 ttttgcagaa acaac
//
LOCUS       DAORKW010000375         3636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1356_length_3636_cov_4.591176, whole genome shotgun sequence.
ACCESSION   DAORKW010000375 DAORKW010000000
VERSION     DAORKW010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1356_length_3636_cov_4.591176"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLB46_12700"
     CDS             <1..982
                     /locus_tag="PLB46_12700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77428.1"
                     /translation="ISVEMVDDCATFIDCLGEIWGPSKLDCEGICNGWHHVGDLNEDS
                     TRAFDDVSLYVDNILDNTILAEICNDANNDSDIDVVDATLVMGCANESLGYTHDVELC
                     ELPQTMFNEIDTVTYSIGSINPAFNYLDIYSHNPTARILSAQFDMEGITIDSVQNLCP
                     ETFGANFQITHTDDEVIAIGYNEVPYQIHAEPIPTFRIFYTTTYPAEICIKHFTAAVN
                     ENFEEVNTKLEDNCKTVEVVLGIPTVTTDILKVKIQPNPFSNSAVFNFENPLNKAFTL
                     ELSDLNGNLLRSYQVQGNSVTIDREDFAAGMYIYRLTGGTASQSGKFVIQ"
     gene            1084..2169
                     /locus_tag="PLB46_12705"
     CDS             1084..2169
                     /locus_tag="PLB46_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRF77429.1"
                     /translation="MRIGFDAKRIFQNATGLGNYSRSVVKNLAEKFPENEYVLFTPNA
                     RNVLPFSSKNENVKTVIGRGSVWRSFGIRRDIVKNKIDVYHGLSNELPFSINKTDAKS
                     VVTIHDLIFEHFPHHYPGVDRTLYDVKSKFAVNHADIIVAASESTKRDIVDYYKVSPD
                     KIHVVYQSCDEVFYDPHNADLSNYHLPSEFMLYVGSVTERKNLLTVCKAYLLIPDAQK
                     IPCVIIGNGKRYAEEVKSFIAEHHLQHWFIFLENIPTPELPAFYRKASCFIYPSLYEG
                     FGIPILEAMVCNCPVITSGVSSLPEVGGDAAAYVNPESPEEIASQILHVLTNAEMRAT
                     MISRGKSRLPIFDKFSLAEKMMALYQI"
     gene            2254..2904
                     /locus_tag="PLB46_12710"
     CDS             2254..2904
                     /locus_tag="PLB46_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77430.1"
                     /translation="MKTAIRVIGIIVLIALALGVFIAIRFPFLTPWYHKKYSAEAYQD
                     EKLRLAAISETDTETCIMSRFETLYPYWLGTKWNFNGITETPGEGSIACGYFVTTMVR
                     DMHFDIPRVKLAQCASEEMIVALVDKTKIKRYNGKENGLMLDYIEEKGRNLYIVGLDN
                     HTGFVLHDGTDIWFIHSSGAFPFCVIKEKIENANVLMDSKYKVLGCLSESAVIWEF"
     gene            complement(2988..3416)
                     /locus_tag="PLB46_12715"
     CDS             complement(2988..3416)
                     /locus_tag="PLB46_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF77431.1"
                     /translation="MSAVHKILDKKGKPLFTITPETSVYHALETLAAHNIGALIVKDG
                     ERFCGVFTERDYARKVVLQDRNSRETSVKEIIDNDAETVSSSASIKDCMTLMTANSIR
                     YLPVIDNGNVLGIISIGDVVKYVIEDQAFTLQQMENYINQ"
     gene            3516..3600
                     /locus_tag="PLB46_12720"
     tRNA            3516..3600
                     /locus_tag="PLB46_12720"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3550..3552,aa:Ser,seq:tga)
BASE COUNT         1172 a          614 c          697 g         1153 t
ORIGIN      
        1 aatttcagtt gaaatggtag atgattgcgc aacatttatt gattgcctcg gtgaaatatg
       61 ggggccatca aaattagatt gcgaaggtat ttgcaacgga tggcatcatg ttggcgattt
      121 aaatgaagat agtacacgtg cctttgatga tgtaagttta tatgtagata acattttgga
      181 taataccatt cttgcagaaa tttgtaatga tgcgaataat gatagcgata ttgatgttgt
      241 tgatgctacc ttggttatgg gttgtgctaa cgaatcgctt ggctatacgc atgatgtgga
      301 attatgtgaa ttgccacaaa ctatgtttaa tgaaatagat actgtaacat atagtatcgg
      361 ttcaataaac cctgcattta attatctgga tatttatagt cataacccta cggcaagaat
      421 attatctgct cagttcgaca tggaagggat aacaattgat tcagttcaaa atctttgtcc
      481 tgaaacattt ggtgctaatt tccaaattac gcatactgat gatgaggtta ttgcaattgg
      541 atataatgaa gtgccttatc aaatacatgc tgaaccgatt ccaaccttca gaatttttta
      601 tactacaaca tatcctgctg aaatctgtat aaaacatttt acggcagcag taaatgaaaa
      661 ttttgaagaa gtaaatacca aattggaaga taattgtaaa acagttgagg tcgtacttgg
      721 cataccaact gttacaactg atattttaaa ggtgaaaatt caacctaacc cttttagcaa
      781 ttcagctgta ttcaatttcg aaaatccgtt aaataaggct tttacccttg aattgagcga
      841 cttgaatggc aatttattaa gaagttatca ggtacaggga aatagcgtaa ctattgaccg
      901 tgaagatttc gctgcaggca tgtatatcta ccgtctcaca ggcggtacag ccagccaaag
      961 cggcaaattt gtgatacaat aacatattta tcctacttca aagccctgac tataacggtc
     1021 agggcttttt ttatacacca tatggtagct actagatgtt aagagaaata gtatcttcgc
     1081 cccatgcgta tcggttttga tgccaaaaga atatttcaaa atgctaccgg attagggaat
     1141 tacagcagga gtgtagttaa aaaccttgct gaaaaatttc ctgaaaacga atacgtgtta
     1201 tttactccca atgccaggaa tgtattgcct ttttcttcaa aaaatgaaaa tgtaaaaacg
     1261 gttattggcc gaggctcagt ttggagaagt tttggcattc gtcgcgatat cgttaagaat
     1321 aaaattgatg tttatcacgg cttgagtaat gaattgcctt ttagcataaa caaaactgat
     1381 gctaaaagtg tagttactat ccacgattta atttttgaac attttccgca tcattatccc
     1441 ggtgtggata ggactttata tgatgtaaaa agcaaatttg cggtaaacca tgcagatata
     1501 attgttgcag caagtgaaag cacaaaacgc gacattgttg attactacaa ggtttctccg
     1561 gataaaatac atgttgttta tcaaagttgt gatgaggtgt tttacgatcc acataatgca
     1621 gatttaagta attatcatct tccttctgaa tttatgctat acgtgggttc ggtaacagaa
     1681 agaaaaaatc ttttaacagt ttgtaaggca tatttattaa ttccggatgc acaaaaaata
     1741 ccttgcgtaa ttattggaaa tggcaaacga tatgctgaag aagtaaaatc atttatcgca
     1801 gaacatcatt tacaacattg gtttatattt ttagaaaaca ttccaacacc tgaattaccg
     1861 gcgttttatc gcaaagcaag ttgttttata tacccaagtt tatacgaagg ttttggtata
     1921 ccaatattgg aagcaatggt gtgcaattgc ccggttataa catccggtgt ttcaagtttg
     1981 ccggaagtag gtggagatgc agctgcttat gtaaaccctg aaagtcctga agaaattgcc
     2041 agccaaattc tccatgtgtt aaccaatgct gaaatgcgcg ccacaatgat aagcagaggt
     2101 aaatccagac ttcctatttt cgacaaattt agcctggcag aaaaaatgat ggctttgtat
     2161 caaatttaat tgagttcatt cattattaca ggtaattcaa taatgtatga tatttaataa
     2221 acagcaacac aaaatcgttt ctacaattac tttatgaaaa ccgcgattcg ggtcattgga
     2281 ataattgttt tgattgcact tgccttaggc gtatttatcg ccattcgttt tccgtttctt
     2341 actccctggt atcacaaaaa atacagcgcc gaagcctatc aggatgaaaa attgcgactt
     2401 gccgctatta gtgaaacaga tacggaaact tgtattatgt cgcgatttga aacattatat
     2461 ccgtattggc ttggcaccaa atggaatttt aacggcataa ccgaaacacc cggtgaaggg
     2521 tcaattgcct gcggatattt tgttaccact atggtgcgtg atatgcattt tgatattcca
     2581 agagttaaac ttgcgcaatg tgcttctgaa gaaatgattg ttgctcttgt tgataaaacc
     2641 aaaatcaaaa gatataacgg taaggaaaac gggttgatgc tcgattacat tgaggaaaaa
     2701 ggtcgcaatt tatatatagt tggactcgat aatcacaccg gttttgtttt acatgatggt
     2761 acagatatat ggttcataca tagcagtggt gcttttccat tttgtgtaat aaaagaaaaa
     2821 atagaaaatg ctaacgtttt aatggattcg aaatataaag tattaggctg tctaagcgaa
     2881 agcgctgtga tttgggaatt ttaagcctac agccaactgc ctaaacttat caaacagaca
     2941 tgttataatt atactttaaa taccaataag cggttaaccc attttgccta ctgattaata
     3001 taattttcca tttgctggag cgtaaaggcc tgatcttcta ttacgtattt cacaacatca
     3061 ccaattgaaa taatacctaa tacattacca ttatctataa caggtaaata gcgaattgaa
     3121 tttgccgtca tcaaagtcat acaatcttta attgaagctg atgatgaaac cgtttccgca
     3181 tcgttatcaa taatttcttt tacggaagtt tcacgtgaat ttctgtcttg caaaacaact
     3241 tttcttgcat aatctctttc agtaaaaaca ccacaaaatc gttcaccgtc ttttacaata
     3301 agtgcaccaa tattatgggc agcaagtgtt tcaagtgcat ggtacacgga tgtttcaggg
     3361 gtaatcgtaa acaatggttt tccttttttg tcaaggattt tgtgcactgc agacatacaa
     3421 taaaatttag ggttaaatga acagttggtg taacaagata cgatattttt taagattatt
     3481 ttaaaacgtg attttgaaat gttacctttg ccctcggaga gatggcagag cggtcgattg
     3541 cggcggtctt gaaaaccgtt gaggtgcgag cctccggggg ttcgaatccc tctctctccg
     3601 caaatcaata aaaacagcac cgttaggagc tttttt
//
LOCUS       DAORKW010000376         3631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1358_length_3631_cov_5.323826, whole genome shotgun sequence.
ACCESSION   DAORKW010000376 DAORKW010000000
VERSION     DAORKW010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1358_length_3631_cov_5.323826"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1485)
                     /locus_tag="PLB46_12725"
     CDS             complement(166..1485)
                     /locus_tag="PLB46_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77432.1"
                     /translation="MKFSKILAVGLAIALPNFLQAQANCAAPNTGLTPFVDLQTGTYM
                     GYQAGMYPGGTNELTGPHLKSGKTIAKGIKPLDGDGNVNFGDGVVLVAGFGPSVPGHI
                     YGKVVEHIRTPSEKYDLNPCLDAINLCVGGKDIGYATDDSTTVDYWELLVQKVYDVGY
                     TPEQVQIGWMYFNAKGLTVPPVFPDKALETMELDIQFINKAKEYFPNLKIVYWSARHF
                     GGYADTDIVEYYSLAEPTSYQNNWTVKWLVESQINMTDPRLQYKGANPKSPYILWGPN
                     FWCDGDIRRFWDDKKYVCELSFDADDGYHLSDQQDSKDALDILDVMYYGEVGKQFTKN
                     SPTWESCIPWLDTLAGGLRVKPEVYGDFKISPNPGHDEFYISLPTESVPAYITIYNEL
                     GIVVDEFLHEENFSTEVLIDMSGKPTGMYFAKVMMENKITTLRFIRH"
     gene            complement(1598..2626)
                     /gene="ruvB"
                     /locus_tag="PLB46_12730"
     CDS             complement(1598..2626)
                     /gene="ruvB"
                     /locus_tag="PLB46_12730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRF77433.1"
                     /translation="MKNPNLDPTDDFMSNSEKEFEKAMRPQGFFEFQGQDRIVDNLKV
                     FIAAAKQRGEALDHVLLHGPPGLGKTTLSFIVANELGVNMKITSGPVLEKPGDLAGLL
                     TSLEERDVLFIDEIHRLSTVVEEYLYQAMEDFKIDIMIETGPNARSIQINLNPFTLVG
                     ATTRSGMLSAPLRSRFGITSRIEYYDAATLQKIVERSSAILNTTITQDGSFEIARRSR
                     GTPRIANNLLRRIRDFAQVKGKGVIDVEIARYGLTALNVDEHGLDEMDNRILSDIIEK
                     FSGGPVGLTTIATAVGEDAGTIEEVYEPFLIQEGYLKRTPRGREATDKAYKHLGKLPP
                     SSNYSLFD"
     gene            2819..>3631
                     /locus_tag="PLB46_12735"
     CDS             2819..>3631
                     /locus_tag="PLB46_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77434.1"
                     /translation="MKKYLLMFSFAPLLFACNQGKIDDLEKQNADLQGKSTEYKAGWD
                     AALNEIGDYMSMMNEIDSNLIEIKRTEGLIDANLNNEGRSKEQQKQDIVANINALNDL
                     IQKNKNLVSELDRKYKGNALKIKELDQKIALLNEQLAQKESDLLALKTELEKANYQIA
                     SLSTDLSTITVQKQELEEQNTQQAEVIAKQTDDLNTAYYIAGTYKELKELGVVDKEGG
                     FIGIGANKELVNNFDASAFTKVDIRKFKELSINSKSAKVITTHSTDSYTLVEG"
BASE COUNT         1197 a          695 c          628 g         1111 t
ORIGIN      
        1 atttaagata tcaggggtca attactaaag ttggtcctcc agccaatatt aatgtggttg
       61 gtttaatcaa tagattagta actgcaagag ccacttatta tttttaacag gcaaaaaaaa
      121 agcgctgaaa gtcaattcaa cgcttttaaa ttatttggtg attaattaat gtctgataaa
      181 tcgcaaagta gtgattttat tttccatcat cactttagca aaatacattc ctgttggttt
      241 tccgctcata tctattaata cttctgtaga gaaattttct tcgtgtaaaa attcatctac
      301 aacaatacct aattcattat atattgtaat ataagctgga acactctcgg ttggcagact
      361 aatataaaat tcatcgtggc ccggatttgg tgaaattttg aaatcaccat atacctcagg
      421 tttaacacgc aaacctcctg ccagtgtgtc taaccaagga atacaacttt cccatgtcgg
      481 gctatttttt gtaaattgtt tacccacttc tccgtaatac attacgtcta aaatatctaa
      541 tgcatcttta ctatcttgct gatcacttaa gtgataaccg tcatctgcat caaacgataa
      601 ttcgcacaca tattttttat catcccaaaa tcttctgata tcgccatcac accaaaaatt
      661 tggtccccat aaaatataag gtgattttgg atttgcacct ttatattgta atcgtgggtc
      721 ggtcatattt atttgtgatt caaccaacca tttcactgtc cagttgtttt ggtaagaagt
      781 tggttcagct aatgaataat attcaacaat atcagtatca gcataaccac caaaatggcg
      841 agcactccaa tacacgattt tcaaattagg aaaatattct tttgctttat taataaattg
      901 aatgtcaagc tccatagttt ctaatgcttt atcaggaaat acaggaggta ctgttaatcc
      961 ttttgcatta aaatacatcc aaccaatttg cacttgctct ggagtatatc ctacgtcata
     1021 aactttttga accaataatt cccaataatc aactgtagtt gagtcatcgg ttgcataccc
     1081 gatatcttta ccaccaacac aaagattgat agcatctaga caagggttca agtcatactt
     1141 ttcagaaggt gtacgtatat gttctaccac cttaccatat atatggccag gaactgaagg
     1201 accaaatccg gcaaccagca ctacaccatc gccaaaattc acattaccat caccgtcaag
     1261 tggtttgata ccttttgcaa ttgttttacc actttttaag tgaggcccgg ttagctcgtt
     1321 ggttccaccc ggatacatac cggcctgata gcccatgtaa gtgccggttt gcaaatcgac
     1381 aaacggtgtt aaaccggtat ttggcgcagc gcaattagcc tgggcctgca aaaaattagg
     1441 gagggctatt gccaaaccta ctgcgagaat ttttgaaaat ttcatgaatt tcatatcaat
     1501 ttattatgtt ttatgggaat acttgcaaag atattaaata ataggccctg cttgttgagg
     1561 ttaagacgaa aattctgata aaaaggtggt atgaccatca atcaaataag gaataattac
     1621 tactcggcgg caattttcct aaatgtttgt atgctttatc agttgcttcc ctgcctctgg
     1681 gggtgcgttt aaggtaacct tcctgaatta aaaacggttc atatacctct tcaattgtcc
     1741 cggcatcttc ccctacagct gtagctatgg tagttaagcc gaccggtcca cctgaaaatt
     1801 tttcgataat atcacttaga atacgattgt ccatttcgtc gagcccgtgc tcatctacgt
     1861 ttaaagcagt gagtccgtat cgggcaattt ccacatcgat aacaccttta ccttttacct
     1921 gagcaaaatc tctaattctg cgaaggaggt tattggcaat acgtggggta cccctcgagc
     1981 ggcgtgcaat ttcaaatgag ccatcctggg tgatggtagt atttaatata gctgatgaac
     2041 gttctactat tttttgcaaa gttgcggcat cataatattc gattcgagag gtaattccaa
     2101 aacgagacct taaaggagca ctcagcatac cggaacgagt agttgcgccg acaagtgtaa
     2161 atgggttcag attaatttga atggaacggg cattaggtcc ggtttcaatc ataatatcaa
     2221 tcttaaaatc ttccattgcc tgatagagat attcctctac cacagtgctg agccgatgaa
     2281 tttcatctat aaataataca tcacgctctt cgaggctggt taataatccg gctaaatcac
     2341 cgggtttttc taatacagga ccggaggtga tcttcatgtt tacccctagt tcattggcaa
     2401 caataaatga aagtgttgtt ttccctaaac cgggaggccc gtgtaataac acatgatcca
     2461 atgcttcacc acgttgtttt gcggctgcaa taaatacttt taagttatct actattctat
     2521 cctgaccctg aaactcaaaa aagccttgcg ggcgcattgc tttttcaaat tccttttcgg
     2581 aatttgacat aaaatcatcg gttggatcta aattcggatt tttcactgag aaacaaagat
     2641 acagtgtagg aaatagaaaa cacaccttaa tattttccac atattgagga gtatttcctc
     2701 ataaaataag tagttacttt tatgagttaa tatgcgaaag tcttgatttt aaaggatttc
     2761 aagtaaaatc ggatttttgg ctcattattt tcaataaaca tatacaaatc taaaaaaaat
     2821 gaaaaagtat cttttaatgt tcagcttcgc gccattactg tttgcatgta accagggaaa
     2881 aattgacgat ctagaaaaac aaaatgccga tttacaaggt aaaagtaccg agtataaagc
     2941 aggttgggac gctgcgctaa atgaaattgg tgactatatg agtatgatga atgaaatcga
     3001 ctcaaacctc atcgaaatca aaagaacaga aggtttaata gatgccaatt taaataatga
     3061 aggccgatca aaagagcagc aaaaacaaga tattgttgct aacatcaatg ccttgaatga
     3121 tttaattcag aaaaacaaaa accttgttag cgaactcgac cgcaaataca aaggcaatgc
     3181 tttaaaaatt aaagaattag atcaaaaaat cgccttgtta aacgaacaat tagcgcaaaa
     3241 agaatccgat ttattagcac ttaaaactga gttagaaaaa gctaattatc aaattgcttc
     3301 actttctacc gatttaagta ccatcacagt tcaaaaacaa gaacttgaag agcaaaatac
     3361 gcagcaagcg gaagttattg caaaacaaac tgacgactta aatactgcat attatattgc
     3421 cggcacttat aaagaattaa aagaattagg tgttgtagat aaagaaggtg gatttattgg
     3481 aataggtgca aataaagaac ttgtaaataa ttttgatgct agtgcattca ctaaagttga
     3541 tattcgcaaa ttcaaagaat tatctatcaa ctcaaaaagc gctaaagtaa ttacaaccca
     3601 ctcaactgac tcttatactt tagttgaagg t
//
LOCUS       DAORKW010000377         3627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1363_length_3627_cov_4.835386, whole genome shotgun sequence.
ACCESSION   DAORKW010000377 DAORKW010000000
VERSION     DAORKW010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1363_length_3627_cov_4.835386"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PLB46_12740"
     CDS             complement(<1..977)
                     /locus_tag="PLB46_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77435.1"
                     /translation="MARALTQQQKIFRNVDFILLNINALLVIIGYGLAVYTDSASVPL
                     VKIIKTAFLAVSLIALLKNLQFKSNPFPSPTIIYLLVFAFWVTLMAFFVEDVVFSIMR
                     AMTFIFPLFYTYLVLTNLANKYDVYTLLKAFARSFNLIYSLPIIVFLLSGAGFSQANI
                     YGQTGNSGHFFISNQYGWACAMFLITGIDVWLNTKPKKLYKYFILGTSIVGLFLVMAS
                     GNRASWVAICLAIIIFILRLKNIRSDFKVLITLIPIVGIIWFYQLPESSIQTRLSDTE
                     TQIDKGEARFNTAKFAFGVFNEDKIKWITGAGMFNYEGTIGSDGLGDYH"
     gene            complement(970..2073)
                     /locus_tag="PLB46_12745"
     CDS             complement(970..2073)
                     /locus_tag="PLB46_12745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRF77436.1"
                     /translation="MKILFCTNAFETVSNGPAKFAHLVLEMQNYFPDCEVRVLTEDCK
                     SPVKNKIYKQQLYIPRIFKPLGMFLRMFQYHKKAMHIKHSDFNFDYLVYNNAIVGIWS
                     GLRYKNTIGFINDDTYATTNWKSFWGLNWNKGRIFFTTEKLACKCLRMIIVNSNYLSA
                     LIQKKYHVPAHKVQRLYKAKEVDNEFNTKSNLVPTILFVKNDYLRGGLFNLVDALKLL
                     STPFKLIIAGPHATSKQSILSYIGQQHQLEVQFKGIVTPIEVCDDMRKADIFCVPSTK
                     EALGVANIEALAVGCTVISTQVGGIPEVLDNGNNGWLVPPNNPKALANAIQTALSNPE
                     LCAEKTKNGHIFVEQFKLGNVLSNFKQLLTNLG"
     gene            complement(2070..3113)
                     /locus_tag="PLB46_12750"
     CDS             complement(2070..3113)
                     /locus_tag="PLB46_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77437.1"
                     /translation="MEHKKTLLYICTNDGSDMRINKELRTLSSNFAITYIGIGQGEVF
                     TFGKQYCQSFHLVKGKRNNPFTIVKLLLKLISLKLSKYDSVHVVNEKLIIFLYPIIFH
                     KHLVVDIFDSIFLINNKSRNKNILLKKILYAPAKTLLVTDENRKSLMPDFAKHKVAIL
                     PNYPYKYEGPVATKQNNQLTILYNGWLGENRGTEFIYKCLEADSELHVIMAGWLADEA
                     SKKLLDHPQVTYKGIMKQDEVLHLAATACDYILCVYAPINENNINASPNKIYDSIQTK
                     TPIITNAEVVVAQFVETNQIGVVIDDYYHFDARELLGVLRARKNQFVFNDTLRTIYTW
                     ENVEGILLAAHSL"
     gene            complement(3103..>3627)
                     /locus_tag="PLB46_12755"
     CDS             complement(3103..>3627)
                     /locus_tag="PLB46_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77438.1"
                     /translation="WNLGPTDYDRFAEAKLMGFDSPFETGANAMFTDRFKSYIIAEPS
                     YYAKTCLYRTGYFLTAPHGWGLEVDKYAKTYSELRNEGAAFGSFPYLLGKYWTQMLSA
                     IFTLISFLSFFVFMRKHPDLNVKLILVSVFLSVYLSHVFIHITSVYLMSLYPIQCFFI
                     AYMTTNLKFRKVGT"
BASE COUNT         1169 a          632 c          608 g         1218 t
ORIGIN      
        1 ttgtgataat caccaagtcc atcggagcca attgtgcctt cgtaattaaa cattcctgct
       61 cctgttatcc attttatttt atcttcatta aatacaccaa aagcaaattt tgcagtatta
      121 aatctggctt cacctttgtc aatttgtgtt tcagtgtctg ataatcgcgt ctgtattgat
      181 gattcgggta attgataaaa ccaaattatc ccgactattg ggattaaggt tattaaaact
      241 ttaaaatcag aacggatatt ttttaagcgt agaataaaaa tgatgatagc caaacaaatt
      301 gctacccagg aggctctatt tccactggcc ataactaaaa aaagtccaac tatactagtg
      361 ccgaggataa agtacttata taattttttt ggtttggtat ttagccaaac atctattccg
      421 gtaattaaaa acatggcaca agcccaccca tattgattgg aaataaaaaa atgacctgaa
      481 tttccagttt gaccataaat atttgcttgt gaaaaacctg ctccacttaa taaaaaaaca
      541 ataatgggta aactataaat aaggttaaat gacctggcaa aagctttaag taaagtatat
      601 acatcgtatt tattcgctaa attggttaat acgagataag tgtaaaataa cggaaaaata
      661 aatgtcatgg cccgcataat ggaaaataca acatcctcca caaaaaatgc cattagtgtt
      721 acccaaaacg caaataccaa taaataaatt atagttggac ttggaaatgg attgctctta
      781 aattgtaagt tctttagcag ggctatcaat gaaactgcta aaaatgcagt tttaataatt
      841 ttcaccaatg gcacagatgc agagtctgtg taaacagcta atccatatcc tattatgact
      901 aataaggcat taatattcag taatatgaaa tccacatttc tgaatatttt ttgttgttgt
      961 gtgagtgctc tagccaaggt ttgtcagaag ctgtttgaaa ttggataaca cattacccaa
     1021 tttaaattgt tcaacaaata tatgcccgtt ttttgttttt tcggcacata attcggggtt
     1081 tgaaagtgca gtttgtatag cgttggctag tgctttaggg ttgttgggcg gaaccaacca
     1141 gccgttattg ccattatcta aaacttccgg tatgccacct acttgagtgc taatcacagt
     1201 gcaccccact gccagggctt caatgttagc tacgcctaat gcttcttttg ttgatggcac
     1261 acaaaatatg tccgcttttc gcatatcatc gcaaacttct attggggtta caattccttt
     1321 aaattgaact tcgagttggt gctgttgtcc aatataagat aatatggatt gtttactcgt
     1381 tgcgtgaggc cctgcaatta ttaatttaaa aggtgttgat aggagtttta acgcatcaac
     1441 caaattgaac aagccacctc gtaaataatc attttttaca aataatattg ttggcaccag
     1501 gttcgatttt gtgttaaact cgttgtcaac ttcttttgct ttatacaaac gttgaacctt
     1561 atgtgcagga acatgatatt ttttttgaat taaagcactt aaataattgg agttgacaat
     1621 aatcatccta aggcatttgc aggcaagttt ctccgtcgta aaaaatattc tccccttgtt
     1681 ccaatttaat ccccaaaacg atttccaatt tgtagttgca taggtgtcgt cattgataaa
     1741 tccaattgta tttttataac gcaatccact ccaaattcca acaatggcat tattataaac
     1801 gaggtaatca aaattaaagt cggagtgttt aatatgcatc gcctttttat ggtattgaaa
     1861 catgcgtaaa aacatgccta gaggcttaaa tatacgcggt atataaagct gttgtttgta
     1921 aattttattt ttaaccggac ttttacaatc ttcggtcaac acgcgcactt cacaatctgg
     1981 aaaataattc tgcatttcta atactaaatg cgcaaatttt gcaggaccat tgcttaccgt
     2041 ttcaaatgca tttgtgcaaa acaatatttt catagactat gagcagccaa taaaatcccc
     2101 tccacatttt cccatgtgta aatggttctt aatgtgtcgt tgaaaacaaa ctggtttttt
     2161 cttgcacgta atacgccaag taattcccta gcgtcaaaat gataataatc atctattact
     2221 actccaattt gatttgtttc tacaaattgt gcaacaacta cttctgcatt agtaattatt
     2281 ggtgttttgg tttgtatcga gtcgtaaatt ttattgggac tggcattaat attattttca
     2341 tttattggag cataaacaca taaaatataa tcacatgccg ttgcggccaa gtgcaacact
     2401 tcatcctgtt tcataatgcc tttgtaagtg acttgtggat gatcaagcag ctttttagaa
     2461 gcttcatccg ctaaccatcc tgccattatt acatgtaatt ccgaatccgc ttccaaacac
     2521 ttataaataa attcagtccc acgattttca cctaaccagc cattgtataa aatggtaagt
     2581 tgattatttt gttttgttgc taccgggcct tcatatttat aagggtaatt tggtaaaatg
     2641 gctactttgt gtttagcaaa atcaggcatc agcgattttc ggttttcatc ggtaactaac
     2701 aaagtttttg ccggtgcgta aagaattttt tttagtaaaa tgtttttatt tctggatttg
     2761 ttgttaatga ggaaaatact atcgaatata tccacaacaa gatgcttgtg aaaaatgatg
     2821 ggatataaaa aaattatcaa cttttcattt acaacatgta ctgaatcgta ttttgaaagt
     2881 ttaagactta taagttttaa taatagttta actatagtaa acgggttgtt gcgtttgcct
     2941 ttaactaagt ggaatgattg acaatattgt ttcccaaacg taaaaacttc accttgccca
     3001 attcctatat aagtgatggc aaagttggac gacaaggttc gcaactcctt attgattcgc
     3061 atatcggaac catcgttggt gcaaatgtat aacagtgttt ttttatgttc caaccttcct
     3121 gaattttaaa tttgttgtca tatacgcgat aaaaaaacat tgaatagggt ataacgacat
     3181 taaatataca gaggtgatat gaatgaaaac atgacttaaa tatactgata aaaaaacgga
     3241 aactaaaatt aatttaacat ttaggtccgg atgttttcgc ataaatacaa aaaatgaaag
     3301 gaaacttatt agggtaaaaa ttgcagataa catttgtgtc cagtattttc cgagtaaata
     3361 tgggaatgaa ccaaaagcag caccttcatt tcttaattcg gaataggtct tggcatattt
     3421 atctacttct agtccccagc cgtgtggtgc tgttaaaaaa taaccagtcc tatataaaca
     3481 tgtttttgca taataggatg gttctgctat aatgtatgat ttaaaacggt cggtgaacat
     3541 cgcatttgcc ccggtttcga atggggagtc aaatcccatt agtttagctt ctgcaaatct
     3601 gtcataatcc gttggaccta aattcca
//
LOCUS       DAORKW010000378         3621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1367_length_3621_cov_4.584969, whole genome shotgun sequence.
ACCESSION   DAORKW010000378 DAORKW010000000
VERSION     DAORKW010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_3621_cov_4.584969"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLB46_12760"
     CDS             complement(<1..441)
                     /locus_tag="PLB46_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF77439.1"
                     /translation="MNKEVVFIVDDEPMHAQMMEDHLASKYPDFKIYKYSTGEECNDN
                     MDKHPTIVVLDYHLDAVNKNARTGLETLIHLKKKYDHIDVIMISGQDKIDVAVECVRE
                     GAFDYVVKGPSAFVRTEQAFEKLFKHRQVIHELAKYKKLNRTLTW"
     gene            complement(473..829)
                     /locus_tag="PLB46_12765"
     CDS             complement(473..829)
                     /locus_tag="PLB46_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRF77440.1"
                     /translation="MDNYKKINLEFLQSFTQNDNAQMAKYINMFLTLAPVSINTMKTQ
                     HECGDWNNLRTTAHSLKPQLAYMGIESLKETILRIEEYAGEAKNPEAIGGLIQTVEAG
                     CNEAFVELRAVIEKIA"
     gene            complement(840..2204)
                     /locus_tag="PLB46_12770"
     CDS             complement(840..2204)
                     /locus_tag="PLB46_12770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF77441.1"
                     /translation="MQLLNLAIINVPLIVEPPSWKTWWIYSICIIVAALIIYYIIRKR
                     MSDAADDQRILREKVREKTRELEQQKERAEDSEKAKEQFLANMSHEIRTPMNAILGMT
                     RLLLEQDPRTDQLKYLNSIKHASDNLLVIINDILDLSKIEAGKINLEKIPFNLRNEIN
                     AVYETMKVNADDKGLNLLTVVDENCPNTIIGDPYRLSQILLNLTGNAIKFTEKGNVTI
                     QTTCTLEDNQVVVKFSVIDSGIGIAKDKLDYIFDMFTQETSSTTRKFGGTGLGLAICK
                     KLIELQGGNITVESEPGKGSIFSFAIPFAIGKDIAEHNAKDDHKQQVVATMKNVRVLL
                     VEDNEFNQMVAVDTLERAIEGAKVTVAGNGKIAIDMLLNNTFDIVLMDIQMPEMDGHE
                     ATKNIRSNSDEKINSIPIIAMTASVIKAEVDKCFESGMNEFVGKPFSVGELLEKMSKL
                     LARQ"
     gene            complement(2176..>3621)
                     /locus_tag="PLB46_12775"
     CDS             complement(2176..>3621)
                     /locus_tag="PLB46_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF77442.1"
                     /translation="NLKPGTYTFNVKAQNADGIWSDPVSYTFTIKKPFWATLIFQITA
                     GILGLGLIVLFNVLRNRRLRRRAAQLETIVTERTAQLEQQKIMAETSAQRAERSEKAK
                     EEFLANMSHEIRTPMNAIMGMTRLLLEDKPLETQMKYLNAIRMSSDNLLVIINDILDL
                     SKIQAGKMELEQIPFNLRSLLNNLGEIMKFKSDEKNIDFKIIIDETLPECVVGDQVRL
                     NQILINLTGNAIKFTEKGHVYIRCRNVISDNNQVTIAFDVEDTGVGIAQDKIDTIFDS
                     FSQADVATTRKFGGTGLGLSISKRLTELSGGQLSVSSELGKGSVFTATIPFPIGTMEI
                     EKESAADLAAVKAGTDLPIRILLVEDNMFNQMVATDSIAKMFANVTIDIAENGKIAVE
                     KISAEDYAVVLMDVQMPVMDGYEAARTIRKLENKQKSKIPIVAMTASVIKSEVDRCYE
                     NGMDDFIAKPFEPKDLRNKILKYATPQPSDN"
BASE COUNT          954 a          717 c          662 g         1288 t
ORIGIN      
        1 ccaggttagg gtgcggttta attttttgta tttagccagt tcgtgaatta cctgacggtg
       61 tttaaatagt ttttcgaatg cctgttcggt gcgcacaaaa gctgatgggc cctttactac
      121 gtaatcgaaa gcaccttcgc gaacacattc aacagcaaca tcaattttgt cctgaccgga
      181 aatcatgatt acgtcgatat ggtcgtattt ctttttaaga tggattaatg tttcgagacc
      241 agtgcgtgca tttttgttta ccgcgtcaag gtgataatct aaaaccacaa ttgttggatg
      301 tttgtccata ttgtcgttac actcctcacc ggtgctgtat ttataaattt taaaatcagg
      361 gtactttgat gcaagatggt cttccatcat ttgagcatgc ataggctcgt cgtcaactat
      421 aaaaactact tctttgttca tatggtattg aattggattt agatatgcaa tattaagcta
      481 ttttttcaat tacggctctt aactccacaa aagcttcgtt acagccggct tctaccgttt
      541 gtatgaggcc accaatggct tcggggtttt tggcttcgcc tgcgtattct tctatacgaa
      601 gaatggtttc tttcaggctt tcgataccca tataggccaa ttgtggcttt aatgaatggg
      661 ccgtggttct gagattattc cagtcgccac attcatgttg tgtcttcatg gtattaatac
      721 tcaccggagc cagcgttaaa aacatattga tatattttgc catttgggca ttgtcgtttt
      781 gtgtgaaact ctgcagaaat tccagattga tttttttgta attgtccatt gttaatcgtt
      841 tattgtcttg ctaataattt actcattttt tctaataatt ctccaaccga aaatggtttg
      901 ccaacaaatt cgttcatacc actttcaaaa catttgtcaa cttctgcttt tatcacgctg
      961 gcagtcattg cgataatagg aatggagttg attttttcgt cgctgttgga acgaatgttt
     1021 ttagtggctt cgtgtccatc catttcgggc atttgaatat ccattaacac gatatcaaag
     1081 gtattgttta acaacatatc gatggcaatt tttccattgc cagcaacagt tacttttgca
     1141 ccttctattg cgcgttctaa tgtgtctacc gctaccattt gattaaattc gttgtcttca
     1201 accagcagga cgcgcacatt tttcattgta gctactacct gttgtttgtg gtcgtctttg
     1261 gcattgtgtt cagcaatatc tttgccaata gcaaatggta tggcgaaact aaatatggag
     1321 cctttgcccg gttcgctttc tactgtaata ttgccacctt gtaattcgat taattttttg
     1381 cagatagcta atcccagtcc agttccacca aatttacggg tagtgctgct ggtttcctgc
     1441 gtaaacatat cgaaaatata atcgagtttg tctttcgcaa taccgatacc actatcgata
     1501 acactaaatt ttacgacaac ctgattatct tcaagtgtgc aagtagtttg tattgttaca
     1561 tttccttttt cggtgaattt gatagcattt cctgtcaaat ttaaaagtat ttgactcagt
     1621 cgataagggt cgccaataat agtgttagga caattttcgt cgacaactgt gagtaggttt
     1681 aatcctttgt cgtcggcatt cactttcatg gtttcataaa cggcattgat ttcgttgcgg
     1741 agattaaaag gaattttttc gaggttaatt tttcccgctt caattttcga taaatcaaga
     1801 atgtcgttga taattactaa taaattatcg gatgcatgtt taatgctgtt gagatatttt
     1861 aactggtcgg ttcgcggatc ctgttccaac aataaacgtg tcatgcctaa aatagcattc
     1921 attggcgttc taatttcgtg actcatattg gccagaaatt gttccttcgc tttttctgaa
     1981 tcttccgcgc gttctttttg ttgttcgagc tcacgtgttt tttcgcgtac tttttcacgc
     2041 aatatccgct ggtcgtcagc agcatcgctc atgcgtttgc gaatgatata ataaataatt
     2101 aaggctgcta caataataca aatactgtaa atccaccacg ttttccatga tggtggttct
     2161 actataagcg gcacattaat tatcgctagg ttgaggagtt gcatatttta aaatcttatt
     2221 acgtaaatct tttggttcaa atggtttggc aataaaatca tccataccat tttcataaca
     2281 tctgtccact tcacttttaa ttaccgaggc agtcatggca acaatcggaa ttttactttt
     2341 ttgtttattt tccaattttc gaattgtgcg tgctgcttca taaccatcca taaccggcat
     2401 ttgcacatcc ataagcacta cagcatagtc ttcggcacta attttttcca ctgcaatttt
     2461 accattttct gctatatcaa tggtcacgtt ggcgaacatt tttgcaatac tatcggtagc
     2521 taccatttga ttaaacatat tgtcttcaac caataaaata cgaataggta aatctgttcc
     2581 tgctttcacg gcagctaaat cggcagcaga ttctttttcg atttccatag tgccaattgg
     2641 gaaaggaatt gttgctgtaa atacagagcc tttgcctaat tcgctgctca ctgataattg
     2701 gcctccggat aattcagtta aacgtttgct aatgctgagc cccaagccag ttccaccaaa
     2761 tttgcgtgtt gtagcaacat cagcttgcga aaaactatcg aaaatggtgt cgattttatc
     2821 ctgtgcaatt cccacaccgg tatcttccac atcaaaggca atggttacct gattattgtc
     2881 ggaaattaca ttgcgacatc gaatatatac gtgacctttt tcagtaaatt taatggcatt
     2941 acctgtcagg ttaataagaa tttgatttaa tcgcacttga tcacccacaa cacattcagg
     3001 taaagtttcg tcaatgataa ttttaaaatc gatgtttttt tcatcggatt taaatttcat
     3061 tatttcacct aaattgttga gtaagctgcg taggttaaac ggaatttgtt ctaattccat
     3121 tttgcctgcc tgtatttttg ataaatcgag aatgtcgtta ataataacca ataaattatc
     3181 tgaagacata cgtatggcat tcagatattt catttgtgtt tccaatggtt tgtcttccaa
     3241 taacagtcgc gtcataccca taatcgcatt cataggtgtt ctgatttcgt gactcatatt
     3301 ggctaaaaac tcttctttgg ctttttctga acgttcggca cgttgtgcag aagtctccgc
     3361 cattattttt tgttgctcta attgtgcggt gcgctcagtt acaatagtct ccaattgagc
     3421 ggctcttctt cgcagacggc gattgcgcaa aacgttaaat aaaacaatta atcccaagcc
     3481 taaaatgccg gctgtaattt ggaaaatgag tgttgcccaa aatggttttt taatggtaaa
     3541 cgtataactc accggatcgc tccaaatgcc atctgcattt tgtgctttga cattaaatgt
     3601 ataagtgccg ggttttaaat t
//
LOCUS       DAORKW010000379         3621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1368_length_3621_cov_4.062815, whole genome shotgun sequence.
ACCESSION   DAORKW010000379 DAORKW010000000
VERSION     DAORKW010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1368_length_3621_cov_4.062815"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..992)
                     /locus_tag="PLB46_12780"
     CDS             complement(132..992)
                     /locus_tag="PLB46_12780"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF77443.1"
                     /translation="MKLLNKLILPVLICVSTIAGAQQKLGLNAVRTYDVTPADYGLVF
                     DTIRVTTDDDASLFGWHLYPGEKNDKSSKKSVIISHDGNINMANSLEQAGKFLGLGYH
                     VFMYDYRGFGKSSDFNVSPKFYIYQQFATDLTGMIEYVKKYHALFSIDLYGYGIGGGL
                     SLGVGANNLKVHNVIADNPYTTFETVQNRYEQKSGTRIMMPIGYNVTLLEPKMALIDK
                     GDHLRGILLIIGEQGNIMTQADMLELQAMKKKVTTIYTVKGVDNDKNFSSNRDEYFKQ
                     IKAFYEKVGD"
     gene            1264..1884
                     /locus_tag="PLB46_12785"
     CDS             1264..1884
                     /locus_tag="PLB46_12785"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRF77444.1"
                     /translation="MRQKLVDILRDKGIADEQVLTAINAIPRHTFMDAAFLEHAYEDK
                     AFQIGEGQTISQPYTVAYMTEWLELEPNMKVLEVGTGSGYQASVLAEMGAKVFTIERF
                     RKLHEKAKQQLYILGYNKVKMYFGDGYEGIPVHAPYDRIIITAAAKEIPQQLLKQLKV
                     GGIIVLPLGNDDDQMMVKMTKTGENDYRKEIGDNFRFVPMLPGKIF"
     gene            2029..2844
                     /locus_tag="PLB46_12790"
     CDS             2029..2844
                     /locus_tag="PLB46_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77445.1"
                     /translation="MKKIMLIICLSALSLSAFAQNMPKAGYSGLGFNVTGLATMAFDN
                     FGQTALSGAQISDPAGVLTGGFDITLDNLIPQNVLMYKRYYEDGLASRFSLGINTISS
                     STFLGDSTFIPGEYQETETKASGFSFGIGAGIEKHMSTEASKVDPFLGVDVMFAMFNG
                     INYSMTDNITGDGYSSSATQDVSYPGGIGLGLNLLGGFNYFFSDNISIGGEVGLGFNM
                     VNMGGSWTSDYVYEFTSNNTTTTTEVNSVGEYKNKVSGVNVNSYGGINLMVYW"
     gene            2982..3578
                     /locus_tag="PLB46_12795"
     CDS             2982..3578
                     /locus_tag="PLB46_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77446.1"
                     /translation="MKKLVSFVTIAVLAISSLTFSGCAKGEEDPFFSIHTRDARLTQA
                     WNMVSMTGTVVTTVDGLTTNVEYEFDGTNLYITTDGVTESFAYIFNMEVKDNGELFSE
                     EFLNNTVTGATIAQSSKTSYWYWGDDNQSKTSVNLDLTGVLAAWLNYDLPRLAYNDMI
                     MSVDYADNYTVYDPVSETYSAASESVLLEVKFDVDLTQ"
BASE COUNT         1124 a          646 c          720 g         1131 t
ORIGIN      
        1 atgtacttcc ccgtaccttc cctccttccc gacttcccgg caaaaaaaac acaaccatta
       61 ctaccaaatc atctccccaa aaaaaaagcc ggtcgcaaaa agcagaccgg ctcaaaaagg
      121 ttaataaaaa attaatcccc aactttttcg tagaaggctt taatttgttt aaaatattcg
      181 tcgcggttgc tgctgaaatt tttgtcgtta tctacacctt taacggtata aatggtagtt
      241 acttttttct tcattgcctg caattcgagc atatcggcct gagtcataat attaccttgt
      301 tcgccaataa ttaataagat tccacggagg tgatcacctt tatcgattaa agccatttta
      361 ggttccaata atgtaacatt ataaccaata ggcatcataa ttctggtgcc tgatttttgt
      421 tcataacgat tttgaacggt ttcgaaagta gtataaggat tatcggcaat aacgttgtgt
      481 acttttaaat tattagcacc aacaccaagt gataaaccgc caccaatgcc ataaccatat
      541 aagtcgattg aaaataatgc gtgatatttt ttcacatatt caatcatgcc ggtgaggtcg
      601 gtggcaaatt gctgatagat ataaaattta ggacttacat taaagtctga gcttttcccg
      661 aatccgcggt agtcgtacat aaatacatga taacctaaac ccaaaaattt accggcttgt
      721 tcgaggctgt tagccatatt gatatttcca tcgtggctaa tgattaccga tttttttgag
      781 gatttatcat ttttttctcc cggatataaa tgccatccga ataagctggc atcatcatcg
      841 gtagtaacgc gaatggtatc gaataccaga ccgtaatcgg caggagttac atcgtaagtg
      901 cgtaccgcat tgagtcccaa tttttgttgt gcgccggcaa tagtggaaac acaaatcagc
      961 accggtaaaa taagtttgtt aagcaacttc ataaatgtta aataaggttt ttaatgtttt
     1021 tgttaaaatt gataccccgt tgtttcaaaa ttcgtgcctg taaaattaaa aagtgcaaca
     1081 ttattttttc agccaaaaaa gtgattgtgg ataattaagt agcaaaagtg gaaagtataa
     1141 tgctcaaagt gttattcatt tgcaatctat ttgacaggtg agtaaaactt gttcaataca
     1201 atttaacatt taggtaatac aaaagcggtg aatcaggcat tagacacata tagacataag
     1261 ggtatgcgcc agaaattggt ggacattttg cgcgacaagg ggattgcaga tgaacaggtt
     1321 ttaacagcaa taaatgccat tccgcgacat acatttatgg atgcagcatt tttagaacat
     1381 gcgtatgagg ataaggcatt tcaaattggt gaaggtcaaa caattagtca gccctacacc
     1441 gttgcttaca tgaccgagtg gctagaactt gagccgaaca tgaaagtgct ggaagtaggc
     1501 acaggcagtg gatatcaggc ctcagtgctg gctgaaatgg gagcaaaagt atttacgata
     1561 gagcggttca ggaaactaca tgaaaaggct aagcagcagt tatatatttt ggggtataat
     1621 aaggtgaaaa tgtattttgg tgatgggtat gaaggtatac ctgtgcatgc gccttacgac
     1681 agaataatta tcacagcagc agcaaaggaa attccccaac aattgctcaa acaactcaaa
     1741 gtggggggta taattgtatt accattgggt aacgatgacg accaaatgat ggtcaaaatg
     1801 accaaaaccg gcgaaaacga ctatcgcaaa gaaattggtg ataacttcag gtttgtccca
     1861 atgttacccg gcaaaatttt ctaaaaaacc tctccaaatc tgcgttttac aggcaactgt
     1921 atatcaaagc gtactatctt aaaacaggat ggtgggcaac ttatctcact ttttccgccg
     1981 ataaccttac tccctgttta cctttgcgtg aaacaaaaac ctatttatat gaaaaaaata
     2041 atgttaatca tctgtctctc agcattaagt ttgtcagctt ttgctcagaa catgcctaaa
     2101 gcaggatatt caggcttagg ctttaatgtg accggcttag ccactatggc ttttgacaat
     2161 tttggtcaga ctgcattatc gggtgctcaa atttcagacc ctgccggtgt gttaacaggc
     2221 ggatttgata ttaccctgga taatctcata ccacagaatg tattgatgta taagcgttac
     2281 tacgaagatg gtttggcaag tcgcttttct ttaggtatca atactatttc ttccagcaca
     2341 tttttgggtg attcaacctt tatacccggt gagtatcagg aaacagagac caaagcatca
     2401 ggcttttctt ttgggatagg tgctggcatt gaaaaacaca tgtccacaga agcgtctaaa
     2461 gtagacccat tcctgggtgt tgacgttatg tttgccatgt ttaacggtat taattattca
     2521 atgactgata acattacagg cgacggctac agcagctcag caactcaaga tgttagctac
     2581 ccaggcggaa ttggtttggg cttaaattta ttaggaggct ttaactattt cttttctgat
     2641 aacatatcta ttggcggcga agtaggtctt ggatttaata tggtgaacat gggtggaagc
     2701 tggacttctg attatgttta tgaatttact tccaataata caaccactac cactgaagtg
     2761 aattcggttg gtgaatacaa aaacaaggtt tcaggtgtta acgttaatag ctacggtggc
     2821 ataaacctta tggtttactg gtaaataata tttttcaata agttttacaa tcccggcctt
     2881 ttggtcggga ttttttgttg attgcaacta ttttggccat aaatcactct ttgtagctgt
     2941 tattccttaa ctttgcgccg cgttaatttt aagaattata tatgaaaaaa ctagtttcgt
     3001 ttgtcacaat cgccgttttg gctataagtt cgttaacctt ttcaggatgt gctaaaggtg
     3061 aagaagatcc attttttagt atccataccc gtgatgcccg tttaacacaa gcctggaaca
     3121 tggtgagtat gacaggcact gttgttacta ctgttgatgg tttaactacc aatgtagaat
     3181 atgaatttga tggcaccaac ttatacatta caactgatgg tgtaactgaa tcatttgcat
     3241 acatttttaa tatggaagtg aaagacaacg gtgaattatt ttctgaagaa tttttaaaca
     3301 atactgttac cggtgctaca attgctcaaa gttctaaaac cagttattgg tattggggtg
     3361 atgacaatca gagtaaaaca agcgttaatt tagatttaac aggtgtatta gctgcatggt
     3421 taaattatga tttacctcgt ttagcttata acgatatgat tatgagtgta gattatgctg
     3481 ataattatac tgtttatgac ccggtaagtg aaacttattc agcagcgtct gaaagtgtat
     3541 tgttagaagt gaaatttgat gtggatttaa ctcaataaat tacgccaact tatttataat
     3601 ttctgaggga tctgtcaaga t
//
LOCUS       DAORKW010000380         3615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1372_length_3615_cov_3.850281, whole genome shotgun sequence.
ACCESSION   DAORKW010000380 DAORKW010000000
VERSION     DAORKW010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1372_length_3615_cov_3.850281"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1754)
                     /locus_tag="PLB46_12800"
     CDS             complement(261..1754)
                     /locus_tag="PLB46_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77447.1"
                     /translation="MNGNWQELHELIHTMSPSEKGYFRKFVGGYSDGEDKVYEYLFDL
                     LDKQSVLDEDIIVARLRTKCKNLHTVRNHLYNQMLKSLSSYYSKKHLTFELREALNNI
                     EILQNRGLHGQADKFIQYGIDKCDNTDSYAYQLLFIQQLKQNIQHQKETMKPAMRKMI
                     SDKIIQSASFILHRQQILDGYNKSVDWLNTDYPLYDDTVREEANLLLLNLSNIVINDD
                     FGYSELNLLYAAMANICRLTNKLTDAIYYQQKTITLIETLDLVKMNRESSYSAALYNL
                     GSMYFEHNHFKELEQLIHKMEQLQFQNKSAALINYTVVYVLRIHLHTGLKQYARITEY
                     AQDAITFFEENNTVPNLNFDFQIRLLGYYINTNQLNKAVDQVNFMLSHSYTHSQPTFH
                     IHVRLMQLIIHYELKNNVLLPSLYRSTYRFMNRYRHTYKTESTILNFLRRTLNSRDKQ
                     DTQKVLNNLLGVLETNAEQLEERGFFRSYFDYIGWLKGNLPEADRRR"
     gene            2134..>3615
                     /locus_tag="PLB46_12805"
     CDS             2134..>3615
                     /locus_tag="PLB46_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77448.1"
                     /translation="MKIRFIFLLPVLLCSTVLVRAQNISINTTGVVADASAMLDITST
                     TKGFLAPRMSSAQRIAIVSPATGLLVYDTTLNLFYMYDGTAWRAILNSITGWTLFGNN
                     GTSPAIHFLGTVDNQPIMLRTNNAHAGMISHGGSVFIGSGAGEVNATNNNAGFGAYTL
                     NTNTSGNYNSAVGYGALFSNTSGSDNNAVGAFVLQLNTTGYSNTAMGTNALSSNLTGF
                     ENQAFGYNALAANTIGTYNVAVGKEALLGNTSGTSNVAIGTNALHQNLTGGYNVAIGS
                     SALYNSTVSNTVAIGFNSMFSNTSGANNTAIGYYTLRGNSTGSNNTATGYYALAVNTT
                     GSDNTATGQNALTSNTTGFNNTAVGSGALRYNTTAFGNTAVGFQSLQDATTGHSNTSV
                     GSYNMENITTGVENAAVGRASLGIGTTASYNAAIGFQSLYSLTTGSANTALGRNALYS
                     ATTATNNVAVGSAALYTTTSSNLTAVGFNAMFFTTTATNNTAIG"
BASE COUNT         1107 a          712 c          684 g         1112 t
ORIGIN      
        1 accccatcac cacccaaaat tatttttccc caccaccgtt ctttgatact gttgtaataa
       61 taaaaaatga tctgcacacg cagaaaaaaa atccgcgcct atctgccgcc atgcaggccc
      121 atccaaaaca catcatcccc aaaaaaaatc cgcgccaatc cgttcaatcc gcgtcatcag
      181 cgcctgtctg cctctggcag gttccctttc aactcacaat catccccaaa aaaaatccgc
      241 gaaaatccgt tcaatccgcg tcatctgcgc ctgtctgcct ctggcaggtt ccccttcaac
      301 cacccaatat aatcgaaata cgacctgaaa aacccgcgtt cctccaattg ttcggcattg
      361 gtttccaata cacccagtaa gttattcagc accttttgtg tatcctgttt atcgcgacta
      421 ttcaaggtgc ggcgtaagaa atttaaaata gtggattcgg ttttataagt gtgacgataa
      481 cgattcataa accggtaagt gcttcggtat aaagaaggca gcaatacatt attttttaat
      541 tcgtagtgaa taattaattg catgagtcgg acatgaatat ggaaagttgg ctgactatgt
      601 gtataggaat gactaagcat aaaatttacc tgatcaaccg ctttgttgag ttgattggta
      661 ttaatataat atcccaataa acgaatttgg aaatcgaaat tgagattggg cacggtatta
      721 ttttcctcaa aaaaagtaat cgcatcttgt gcatattcgg taatgcgagc atattgtttt
      781 aacccggtat gcaaatgaat gcgtaaaaca taaacaacag tataattaat taatgcagca
      841 gatttattct ggaattgtaa ttgttccatt ttatgaatca actgctctag ttctttgaaa
      901 tgattgtgtt cgaaatacat tgaaccaagg ttgtacaagg cggcactgta actgctttcc
      961 ctgttcattt tcacgaggtc gagggtttcg attaaggtta tggttttttg ctggtaataa
     1021 atagcatctg tcagtttatt agtgaggcga caaatattgg ccatggctgc gtataataaa
     1081 ttgagttcac tgtagccaaa gtcatcgtta attacaatgt tcgacaaatt gagtaataac
     1141 aaattggctt cttcccgcac agtgtcatca tacaaaggat aatccgtgtt tagccagtcg
     1201 acagatttat tgtaaccatc taaaatttgc tggcggtgta aaataaatga tgccgattga
     1261 ataatcttat cgcttatcat tttccgcatc gccggtttca tggtttcctt ttgatgctga
     1321 atattttgtt tgagttgctg gataaataat aattgataag cataggaatc agtattgtca
     1381 catttatcga taccatactg aataaattta tccgcctgac catgaagtcc tctgttttgt
     1441 aaaatctcta tattatttaa cgcttctcgt aactcgaaag tgagatgttt tttgctgtaa
     1501 taactactca aacttttgag catttgatta tacaaatgat tgcgaaccgt atgcaggttt
     1561 ttacatttag tcctcaagcg cgccacgata atatcttcat ccaatacgga ttgtttgtcg
     1621 agcaggtcga acaggtattc ataaacttta tcctccccat cgctgtaacc gccgacaaat
     1681 ttcctgaaat accccttctc ggaggggctc atggtatgta tgagttcgtg cagttcctgc
     1741 caatttccat tcataatccg aaatgtaaaa taaaagttaa ataaagtatt gaaaatcaat
     1801 aagatatgat gtattaacat ccttccgaaa gggtaaattt aaacaaatta tggcattgtg
     1861 ggaactctat tatcactgtt tctttggttt cacattcact ttcgcaaatt gaatagaaca
     1921 atgaacttag aagaaacagt aagcccggta ttcctcatac cattgctcag gaagaacatc
     1981 tgctacctca tgaagtgtgc atcaactcat tcattctatc caatgcgaag tgaattagat
     2041 tgaattatca acctctgttt cggcaggggt tgattttatt tcaattgcgc aacagttcgc
     2101 tgttgttatt attaaacgcg taaactgata gttatgaaaa tccgtttcat ttttttactc
     2161 ccggtattgc tttgttcaac tgtattagtt cgggcacaaa acatttccat caatacaacc
     2221 ggtgtggttg cagatgcttc agctatgctg gatattacat ctactactaa aggcttttta
     2281 gcaccgcgta tgagcagtgc acaacgtatt gccattgttt cacccgcaac cggattattg
     2341 gtgtatgaca ctactttaaa tttattttat atgtacgacg gcactgcctg gagagcaata
     2401 ttaaatagta taaccggttg gaccttattt ggaaataatg gtaccagtcc agcaatacat
     2461 tttttaggca ccgtagataa tcaacccatc atgttaagaa caaataatgc gcatgcaggt
     2521 atgattagtc atggcggatc cgtttttata ggttcgggtg caggtgaagt aaatgcaact
     2581 aacaacaacg ccgggtttgg cgcttatact ttaaatacaa atacctccgg taattataac
     2641 agtgcagttg gttatggagc attattcagc aacactagtg gttctgataa taatgctgtt
     2701 ggtgcttttg ttttacaatt aaatacgaca ggttatagta atacagctat gggtacgaat
     2761 gcactttcat caaatttaac aggatttgaa aatcaggctt tcggatacaa tgcattagct
     2821 gctaatacaa taggtacata taatgtcgct gttggtaagg aagccttact tggaaataca
     2881 tccggaacat caaacgtggc gattgggaca aatgcattac atcaaaatct tactggtgga
     2941 tataatgtgg cgataggcag ttctgcctta tataattcca cagtaagcaa tacagttgcc
     3001 attggtttta atagtatgtt tagtaataca agcggcgcaa ataatacggc aattggatat
     3061 tacactttac gaggcaactc aacaggtagt aataatacag caaccggata ttatgcctta
     3121 gcggttaata caactggaag cgataatacc gcaactggtc agaatgcatt aacgtcaaat
     3181 acaaccggtt ttaataatac agcagttggg tcaggggcac tgcgttataa cactactgct
     3241 tttggaaata ccgctgtcgg atttcaatcg ttacaagatg caacaacagg gcatagtaat
     3301 acctcagtag gaagttacaa tatggaaaat atcaccactg gagttgaaaa tgccgctgtc
     3361 ggtcgcgcat cactcggaat cggaacaaca gcaagttata atgccgcaat tggatttcaa
     3421 tcgttgtatt ctttaactac aggaagtgcg aatacggcgt taggacgtaa tgctttatat
     3481 tctgcaacaa ctgcaactaa taatgtggct gttggaagtg cagccttata taccaccacc
     3541 tcaagtaatt taactgctgt cggatttaac gccatgtttt ttacaactac tgccactaat
     3601 aatactgcca ttgga
//
LOCUS       DAORKW010000381         3613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1373_length_3613_cov_4.472175, whole genome shotgun sequence.
ACCESSION   DAORKW010000381 DAORKW010000000
VERSION     DAORKW010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1373_length_3613_cov_4.472175"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PLB46_12810"
     CDS             complement(<1..1032)
                     /locus_tag="PLB46_12810"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRF77449.1"
                     /translation="MNKRKIINDPVYGFITIPYEIIYDIVEHPWFQRLRRIQQLGLSH
                     LVYPGAIHTRFHHALGAMHLMGKAINVLRTKGKEITEKEAEAATIAILLHDIGHGPFS
                     HALEKTILTNVHHEEMSILFMQKLNQQLNNRLALAIDIFTGKYHKKFLHQLVSSQLDV
                     DRLDYLTRDSYFTGVHEGVIGYDRLIEMMDVENDELVIEQKGIYSVEKFIIARRVMYW
                     QVYLHKTVICAEQMLIKTLKRAKYLAKSGTHLFATPALHFFLYHDVELKDFNTNVELL
                     DMYAHLDDTDIYASLKEWERCDDKVLSFLSRGIINRKLFRIELESDPISAQKINKITS
                     SVKSAYNIQD"
     gene            1153..2688
                     /locus_tag="PLB46_12815"
     CDS             1153..2688
                     /locus_tag="PLB46_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HRF77450.1"
                     /translation="MLWADDEIDLLKPQILFLEEKGYDVVAVSNGQDAIDKCTDPQVD
                     LIFLDEGMPGLSGLETLQLIKEKRPSVPIVMITKNETEGLMEEAIGSQISDYLIKPVK
                     SQQILLAIKKIVDNKRLVSEKTDSNYQKEFQKIFFTLQDALDAEQWFDIYKKLVHWEI
                     QLDKTGATNMKEVLTSQKAEANVEFGKFIDKNYINWVNGKTKGPVMSHNLLTEKVFPL
                     MTDKVPTFFILIDNLRFDQWKIIEPMISESFKLKSEDYFYSILPTATQYARNAIFAGM
                     MPSEIEKRLPSLWLNDEDEGGKNMQEPEFLKDQIVRNRLNFKYTYHKILNHAEGKQLE
                     ENILNLMTSDFNAIVYNFVDMLSHARTEMEVLKELASDETAYRSLTRSWFDHSPLYNA
                     LRKLAEKKVRIIITTDHGTIRVNTPSKVVADRNTTTNLRYKSGKNLQFNKKDVFEVKN
                     PGDACLPKQHISSTFIFAKEDCFFAYPNNYNYYVNYFKNTFQHGGVSLEEMIVPFAVF
                     ESF"
     gene            2772..3203
                     /gene="tsaE"
                     /locus_tag="PLB46_12820"
     CDS             2772..3203
                     /gene="tsaE"
                     /locus_tag="PLB46_12820"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRF77451.1"
                     /translation="MEQTWLSHSVDDLPRIAEELIAAISPLKKIAFYGQMGAGKTTLI
                     KSICLVLGVTEPTSSPTFSIVNEYKGKSKIYHFDLFRINNPTELQEIGFIEYLENNDY
                     VFIEWPEIIEPVYEDYQFVQIHITTISTQERKIHVTWNNNN"
     gene            3215..>3613
                     /locus_tag="PLB46_12825"
     CDS             3215..>3613
                     /locus_tag="PLB46_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77452.1"
                     /translation="MEAKKVLRQIRMLILFFMFGLLFAGITAIPLISELTWLLAHDNI
                     FPSWLVIWMQGVLEGLTATEKQYPFIIYGIDWLAYAHIVIAILFIGVYKDPIKNIWII
                     EWAMWCCVIIFPVAFIFGPIRHIPFEHQLID"
BASE COUNT         1174 a          592 c          683 g         1164 t
ORIGIN      
        1 atcctgaata ttataagccg atttgacaga actagtgatt ttattgattt tctgggcact
       61 gataggatca ctttccagtt cgatgcggaa caatttccgg ttaataattc ccctgctcag
      121 gaaggaaagt actttgtcgt cgcatcgctc ccactctttt agggatgcat aaatgtcggt
      181 atcatcgaga tgtgcataca tgtccaacaa ctcaacattg gtgttgaaat cttttaattc
      241 tacatcgtga taaaggaaaa aatggagtgc aggggtagca aacaggtggg ttccactctt
      301 ggccagatac tttgctcgct tcaacgtttt aatgagcatc tgttcggcac aaatcactgt
      361 tttatgcaaa tacacctgcc aatacattac cctgcgtgcg atgatgaatt tttctactga
      421 ataaatgcct ttttgttcga ttaccagctc atcattttct acatccatca tctcgataag
      481 cctgtcgtaa ccaattacac cttcatgtac acctgtaaaa taactgtcac gggttaaata
      541 atcgaggcgg tcaacatcca actgactgct caccaattga tgaagaaact ttttgtggta
      601 tttgcctgtg aaaatatcga ttgcgagcgc gagtcggttg ttgagttgct gattgagttt
      661 ttgcatgaac aaaatggaca tctcttcgtg atgcacatta gtgagaatag ttttttccaa
      721 ggcatgtgaa aatgggccgt gaccaatatc atgcaacaaa atggcgatag tagccgcttc
      781 tgcttctttt tcagtaattt ctttgccctt tgtgcgcaat acgtttatag ccttgcccat
      841 caaatgcata gcacctaaag cgtggtgaaa acgcgtatga attgctccgg ggtaaaccaa
      901 atggctgagg cctaattgct gaattctgcg aaggcgctga aaccagggat gttctactat
      961 atcataaata atttcgtagg gaatggtaat aaaaccataa accggatcgt ttattatctt
     1021 tcttttattc aaaacttgtt gtttatagtt agtttttact taccttgaag tgttgcaaag
     1081 cggatataca cctatctttg cattttattt gaacgttgca aaattactgt atttctgaag
     1141 aatgatataa ccattttatg ggctgatgat gagattgatt tattaaaacc acaaattctg
     1201 tttttggaag aaaagggata tgatgtagtt gcggtaagta atggacaaga tgccatagac
     1261 aaatgtacag atccacaggt ggatttgatt tttttagatg agggtatgcc aggtttatcc
     1321 ggccttgaaa ccttacaatt gattaaggaa aaacggcctt cggttccaat tgttatgatc
     1381 actaaaaatg aaacggaagg tttgatggaa gaagccattg gttcgcaaat cagcgactat
     1441 ttaattaaac ctgttaaatc gcaacaaatt ttacttgcaa ttaaaaaaat tgtagacaat
     1501 aaacgtttag tatctgaaaa aacagatagt aattatcaga aagaatttca aaaaatattt
     1561 ttcactttac aggatgcatt agatgcagag caatggttcg acatttataa gaaattagtg
     1621 cattgggaaa ttcaattaga taaaacaggt gctaccaata tgaaagaggt gcttacctct
     1681 caaaaagctg aagctaacgt tgaattcgga aagtttattg ataaaaatta tatcaactgg
     1741 gttaatggga aaacaaaagg tccagtaatg tcgcataatt tattaacgga aaaagtattt
     1801 ccattaatga cagataaagt gcctacgttt tttatattaa ttgataattt acgattcgac
     1861 cagtggaaaa taattgagcc aatgatttcc gagtcgttta aattaaaatc ggaggattat
     1921 ttttatagta ttttaccaac ggcaacacag tatgcgcgta atgccatttt tgccggtatg
     1981 atgccgagtg aaattgaaaa aaggttacct tctttatggt tgaatgatga agacgagggc
     2041 ggtaaaaaca tgcaggaacc agagttttta aaagaccaga ttgtccgcaa tcgtttaaat
     2101 ttcaaatata cttatcataa aattttaaac cacgcagaag gtaaacaact tgaagagaat
     2161 atattaaatt taatgacaag cgattttaat gcaattgttt acaattttgt agatatgctt
     2221 tcgcatgccc gaacagaaat ggaagtacta aaagaattgg caagtgatga aactgcttat
     2281 cgttcattaa ccaggtcgtg gttcgaccat tcccctttat acaatgcttt gcgcaaatta
     2341 gcggaaaaga aagtgcgcat cattattact actgatcatg ggacaattcg cgtaaataca
     2401 ccaagtaaag ttgttgcaga taggaatacg acaaccaatt tgcgatataa aagcggtaaa
     2461 aatttacagt ttaataaaaa ggatgtattc gaggtgaaaa acccgggaga tgcttgttta
     2521 cctaaacaac atatcagcag cacatttatt tttgctaaag aagattgttt ttttgcctat
     2581 cctaacaatt ataattacta cgttaattac ttcaaaaata cctttcagca tggtggtgta
     2641 agtttagaag aaatgatagt gccatttgct gtttttgaaa gtttctaaat aattcacttt
     2701 ttgtgaaata ataagatcaa ttttatgaaa ataatcatgg taatccgcca tgattatttt
     2761 aattttaccc cgtggaacaa acatggctat cgcatagtgt tgatgattta cctcgcattg
     2821 cagaggagct gatagctgcc atctccccat taaaaaaaat tgcattttat ggtcaaatgg
     2881 gtgccggtaa aacaacgttg attaaatcca tttgtttggt tttaggcgta accgaaccta
     2941 cctcctctcc tacattttct attgtaaatg aatataaggg caaatcgaaa atatatcatt
     3001 tcgatttatt tcgaataaac aatcctaccg aattacaaga aattgggttt atcgaatatc
     3061 ttgaaaacaa tgattacgta tttatagagt ggccggaaat aattgaaccg gtttatgaag
     3121 attatcaatt tgtgcaaatt catattacta caatttctac acaagaaaga aaaatccacg
     3181 tcacctggaa taataacaat taattttaca atttatggaa gcaaaaaaag tgttacgaca
     3241 aataagaatg ctgatacttt ttttcatgtt tgggttattg tttgcgggca ttacagctat
     3301 tccattaatt agtgaattaa cttggttatt agcgcatgat aatatatttc cttcatggtt
     3361 ggttatttgg atgcaaggtg tgcttgaggg tttgacggct acagaaaaac aatacccatt
     3421 tattatttac ggaattgact ggcttgctta cgcacatatt gttatagcca ttttatttat
     3481 cggtgtatac aaggacccta ttaaaaatat ttggattatt gaatgggcta tgtggtgttg
     3541 tgttatcata tttccggttg cttttatttt cgggccaatc agacatatac cttttgaaca
     3601 tcagcttata gat
//
LOCUS       DAORKW010000382         3598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1383_length_3598_cov_6.119673, whole genome shotgun sequence.
ACCESSION   DAORKW010000382 DAORKW010000000
VERSION     DAORKW010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1383_length_3598_cov_6.119673"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PLB46_12830"
     CDS             <1..735
                     /locus_tag="PLB46_12830"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HRF77453.1"
                     /translation="RPLRGSVKHIDGKTLATLQKILTTIRQKQPNFLRSAGTTQLNNY
                     LEFERNWGLGSSSTLLSNIAQHAKVDAFELNKSIFKGSGFDIACAQSSMPIFYKIDEE
                     GIVFDSVHFNPPQPEQFVFVHLGNKQDSKTEVETFLQSGDIVKKFANEIEIISEISEA
                     LLFCDDFNDFMQLLDEHEEVMQYVLQEEKVKTKMFSDFNGVVKSLGAWGGDFVLAASS
                     MDAAAITDYFKAKELHTIIPYEQMVL"
     gene            807..1856
                     /gene="mvaD"
                     /locus_tag="PLB46_12835"
     CDS             807..1856
                     /gene="mvaD"
                     /locus_tag="PLB46_12835"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HRF77454.1"
                     /translation="MSTATITWQSPSNIALIKYWGKHGNQLPNNASLSLTLNQAATTT
                     SLSLKKKPKKAAIEVDFTFEGEKNEKFAEKIIQYLESIKNDFPYLTTNKLVIESANSF
                     PHSAGIASSASSMSALALCILSQSMIADKKEYKPEEFYQKASYYARLGSGSAARSVFP
                     DFSVWGKSSHIKAGNDKYAIQYPNKFNSDFNDLQDSILIVNEKEKSVSSRAGHALMNH
                     HPMAAQRYKNAESRMKDILSALTKGDWELFCSTVETEALELHALMMTSNPSFILMKSG
                     TLEIIERVRRFRNETKIPVCFTLDAGPNVHLIYPYFEKKQVRNFIQEELLTFCTKKKV
                     IYDSMGNGPQLHIDE"
     gene            1885..2679
                     /locus_tag="PLB46_12840"
     CDS             1885..2679
                     /locus_tag="PLB46_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77455.1"
                     /translation="MLFGEYAVIAGGEGLAIPYPTFSAQWKINTSSPSEYKPHLENFL
                     VWLKEHNFDSILQLSAFENDLQQGLDMASNVPVGYGVGSSGVVVAAIYDKYVIQREND
                     IQTLKKLLASMENYFHATSSGLDPLVCYLNKAVHVKSNGIDITSSDFQPEKIRLELID
                     CGNARSTQQLVNVFKEKMLAPSFAAIMQDYIHLSNTCINAWLSNNEALLIKHLKLLSA
                     LQLQYFEFAIPEKQRKEWKNKLDSNTGYMKLCGAGGGGFLINFATV"
     gene            complement(2690..>3598)
                     /locus_tag="PLB46_12845"
     CDS             complement(2690..>3598)
                     /locus_tag="PLB46_12845"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase"
                     /protein_id="HRF77456.1"
                     /translation="INDPAKDDEVFLPNADRLWNASKYAAKLENLSKVIATGADMLPT
                     LVGLCEVENEMVVKDLVNTNNLKPGNYDYVHYDSPDERGIDVALLYNKNDFKVKSSKA
                     IAVTLPGEELDFTRDILMVNGEISINGKKEQLFVFVNHWPSRSEGEEVSAPKRAAAAT
                     KLKKVVDSLNAKLDNPNIIIMGDFNDTPLDISIQKILNAQATADTYADETLVDLMTKL
                     QTTGQGSYNYKGNWQALDQIIVSSGLLDGKSLEVSPETALFVKKDWMLYHNDKYGDSP
                     DRTFAGNKYIGGYSDHLPVFVGLNIK"
BASE COUNT         1180 a          633 c          650 g         1135 t
ORIGIN      
        1 cgtccgctga gaggaagtgt aaaacatatt gatggaaaaa cacttgctac attacaaaaa
       61 atattgacca ccatacggca aaagcagccg aattttttgc gttcagcagg tacaacacaa
      121 ttaaataatt atttagaatt tgaacgcaac tggggcttag gaagtagttc tacattgtta
      181 tctaatattg cacaacatgc aaaggttgat gctttcgaat taaataaaag tatttttaaa
      241 ggaagtggtt ttgacatcgc ctgtgcacaa tcatccatgc ccatttttta taaaattgat
      301 gaagaaggta ttgtattcga ctcggtgcat tttaatcctc cgcagcctga acaatttgta
      361 tttgtacatt taggtaataa acaagattcc aaaactgaag tagaaacatt tttgcaatca
      421 ggcgatatcg ttaaaaaatt cgctaacgaa attgaaatca tttctgaaat ttcagaagca
      481 cttttgtttt gtgatgattt taatgatttc atgcaattac tggatgaaca tgaagaagta
      541 atgcaatacg ttttacagga agaaaaagta aaaaccaaaa tgttcagcga ttttaatggc
      601 gttgtaaagt cgctcggagc ctggggtggg gattttgtat tggcagcttc atcaatggat
      661 gctgcagcaa ttacagacta tttcaaagca aaagaactgc atacaattat tccatatgag
      721 caaatggtat tgtaaacccg gagccgaagt aagccacttt catccttccc actaatataa
      781 gagcctgcgt tgtatctttg tggtatatga gtactgcaac cattacgtgg caaagcccgt
      841 cgaacatagc attgattaaa tattggggta aacatggtaa tcaattacca aacaatgcct
      901 ctttatcgct tacattaaac caagcggcga caaccacttc gctttcttta aaaaagaaac
      961 ccaaaaaagc agcaatcgaa gtagatttca cttttgaagg agaaaaaaat gaaaaatttg
     1021 cagaaaaaat cattcaatat ctggaaagta tcaaaaacga ttttccttat ctgacaacaa
     1081 acaaactggt aattgaatct gccaacagtt ttccgcattc agcaggtatt gcaagtagcg
     1141 cttcttcaat gagtgctttg gctttatgca ttttatcaca atcgatgatt gcagataaaa
     1201 aagaatacaa acctgaagag ttttatcaaa aagcttctta ttacgcacgt cttggaagcg
     1261 gcagcgcagc aagaagtgtt ttccctgatt tttcagtttg ggggaaatca tcacatataa
     1321 aagcgggcaa cgataaatat gctattcaat atcccaataa atttaatagt gactttaacg
     1381 atttacagga tagtatttta attgtaaatg aaaaagaaaa atcggtaagc agtcgcgccg
     1441 ggcatgcatt aatgaaccac catccaatgg ctgcacaacg ttataaaaat gcggaaagca
     1501 ggatgaaaga tattttatct gctttaacca aaggtgattg ggaattattt tgttcaactg
     1561 ttgaaactga ggcgttggaa ttacatgctt tgatgatgac cagcaatcca tcatttattt
     1621 taatgaagtc cggtacgctc gaaataattg aacgtgtgcg caggtttaga aatgaaacaa
     1681 aaattccggt atgttttact ttagatgcag gacctaatgt gcatttaatt tatccttatt
     1741 ttgagaagaa acaagtacgc aatttcattc aggaagagct gcttacattc tgtactaaaa
     1801 agaaagtaat ttacgatagc atgggtaatg gccctcagtt gcacatagat gaataatcaa
     1861 tccttaaact acccttctaa attaatgctg tttggcgaat acgcagtaat tgcgggcgga
     1921 gaaggactgg ctattccgta tccaactttt tctgctcaat ggaaaattaa cactagttca
     1981 ccaagtgaat ataaaccaca tttagagaat tttttggtgt ggttaaaaga acataatttc
     2041 gattcgatac ttcagttatc tgcttttgaa aacgatttac aacaaggttt agacatggct
     2101 tccaacgtac ctgttggtta tggtgtaggg agctcaggcg ttgttgtagc tgctatttac
     2161 gataaatatg ttatccaacg tgaaaacgat atccaaacat taaaaaaact acttgcttcc
     2221 atggaaaatt attttcatgc aacaagtagt gggctggatc cactggtttg ttatttaaat
     2281 aaagctgtac atgtaaaatc aaatggtatt gatattactt catctgattt tcaaccggaa
     2341 aaaattcgtt tagaattaat agactgtggt aatgctcgtt ctacacaaca attggtaaat
     2401 gtttttaaag aaaaaatgtt agcgccttca tttgcagcaa taatgcagga ctatattcat
     2461 ttatccaata cctgtataaa cgcctggtta tcgaataatg aagcgttatt aattaaacac
     2521 ctaaaattat tatctgcatt acaattgcag tatttcgaat ttgctattcc tgaaaaacaa
     2581 aggaaagaat ggaaaaataa attagacagc aatactggat acatgaaact ctgtggagcc
     2641 ggtggtggtg ggttcctgat taattttgct actgtttaaa caataacact tatttaatat
     2701 tcaatccaac aaataccgga agatgatctg agtatccgcc aatatattta ttaccggcga
     2761 aagttctatc aggagaatca ccatatttat cgttatggta taacatccag tcttttttta
     2821 caaataaagc agtttcagga ctaacttcca atgatttacc atccaataaa cctgatgata
     2881 caattatttg gtccaaagcc tgccagttgc ctttataatt gtaagaaccc tgtccggtgg
     2941 tttgcaattt tgtcattaaa tctacaaggg tttcatctgc atatgtatca gctgttgcct
     3001 gggcatttaa tattttttga atactgatat caagtggtgt atcattaaag tcgcccatga
     3061 taataatatt cggattatcc aattttgcat ttaacgaatc aaccaccttt tttaatttgg
     3121 tagcagctgc tgcacgtttt ggtgcactta cttcttcgcc ttctgagcga ctcggccaat
     3181 ggttgacgaa aacaaataat tgttcttttt taccattgat ggatatttcg ccatttacca
     3241 taagaatatc tctggtaaaa tctagttctt cgccgggtaa tgttactgca atcgccttcg
     3301 atgatttaac tttaaaatca tttttattat ataacaatgc cacatctatt ccacgttcgt
     3361 ctggtgaatc ataatgcaca taatcataat tgcctggttt tagattattg gtgttcacta
     3421 aatctttaac caccatttca ttttcaactt cacaaagtcc aaccagggta ggaagcatat
     3481 cggcgccggt tgcaattact ttggataaat tttccaattt cgctgcatat ttgctcgcat
     3541 tccagagtct gtctgcattt ggcagaaata cttcatcgtc ttttgccgga tcgttgat
//
LOCUS       DAORKW010000383         3572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1393_length_3572_cov_5.516349, whole genome shotgun sequence.
ACCESSION   DAORKW010000383 DAORKW010000000
VERSION     DAORKW010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1393_length_3572_cov_5.516349"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="PLB46_12850"
     CDS             <1..1157
                     /locus_tag="PLB46_12850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRF77457.1"
                     /translation="EDNYFYRSLGEKRYELSNHLGNVLSVISDRRLAFDTDANGTTNY
                     YESDVMSAQDYDPFGMLLVGRSWSVSYRYGFNGKEMDAETYGQGNIYDYGFRIYNPRL
                     GKFLSVDPLAATYPWYSTYHFASNNPIFFIDIDGLEGGSSQPGSTVNTGTYLVLDEQG
                     EQVPLNLAVVEIRPAPENNKPNWLKVLNIHNVLDVLGAIDPTGVIDVAHGLIYLAKKD
                     YVNASISFISAAPGGDVVKTLRLADDSKVIAKIIKTADVASTGKKAADDLPKFEREMV
                     ELADELEVKFGKTKTAVDGTKKHTEFGKRVKEMGYEDVQVEQPYLGGRKIESSVGGSS
                     RPDITIVDDAGTPTNVYDYKTGNAKVRESQRRKNESNTGVKHTGTLRATH"
     gene            1175..1843
                     /locus_tag="PLB46_12855"
     CDS             1175..1843
                     /locus_tag="PLB46_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77458.1"
                     /translation="MNKPDIIKFIKKYINPYFTEYMQDSIYLLRVSKDQLFIYGYIFD
                     RNSAPENIINCRAFIVPMFYPTDFIYLDYCSEIKYIGKGNNIDLKTPIDPEVVNWLIG
                     QLNDRRIFFESYVGANKFLKYFEDFHSTLYKDPVSIFAAILSNKQLFEHDFEVFYAEA
                     RPRMNDVAFIAERFRIISEIHQLYKVAPETAKNHLLATAEISFKKLKLNKVLTHSLEH
                     PNGI"
     gene            2357..2803
                     /locus_tag="PLB46_12860"
     CDS             2357..2803
                     /locus_tag="PLB46_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77459.1"
                     /translation="MLRVFPFLATGLLNVFLILGLSYDFAFDYRVHDAYFTLSNGHAL
                     FIFAIIASYFITFIWYVKFTIKLHYFYLWLLSALILIFIVVLPSSFGFDIQELENIPN
                     RKIKYLTFFNFIVQFKLFIFLQLCVNIYILHEVDKKYNNKSSVYKN"
     gene            3046..>3572
                     /locus_tag="PLB46_12865"
     CDS             3046..>3572
                     /locus_tag="PLB46_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77460.1"
                     /translation="MKPNFLKCDSEILVSNNQISSLIKTISPNCLIKSYTNGGYDISE
                     NEIFSILKFALGGYIHHDYKSTGELQCQWNRTNIHNVIIDREPINLELKSKEIIDNYF
                     KPVINNLIPQLNSLSKIDAILNNFNELKLPVVEPPALSVCFPYSQQLVMGLLLANFLN
                     RKDKVQLTKKYLELAS"
BASE COUNT         1250 a          468 c          635 g         1219 t
ORIGIN      
        1 cagaagataa ctatttttac cgaagcttag gtgaaaaacg atatgaatta tctaatcacc
       61 tcggaaacgt tctttcagtt ataagcgacc gccgcttagc atttgacaca gatgccaatg
      121 ggactactaa ttattatgaa tcagatgtga tgtctgccca ggattatgat ccgtttggga
      181 tgttgttggt gggaaggagt tggagtgtaa gttataggta cgggtttaac ggaaaagaga
      241 tggatgctga aacttatggt caaggtaata tctatgacta tgggtttagg atttataatc
      301 caaggctagg gaagttttta agtgtggatc cacttgctgc gacttatcca tggtattcta
      361 cctatcattt tgcaagtaat aaccctatat tttttattga tattgatgga ttagaaggtg
      421 gatcatcaca gccaggttca actgtcaata cgggaactta tcttgttctc gacgagcaag
      481 gcgagcaagt tcccctgaac ctggccgtcg tggaaatcag accagcccct gaaaataata
      541 agcccaactg gttaaaagtc cttaatatac ataatgtatt agatgtctta ggtgccattg
      601 atccaacggg cgttatagac gtcgcacatg gcttaatata tttagcaaaa aaagattatg
      661 taaatgcttc aataagcttt atttctgcag ctcctggtgg agatgtagtt aaaacattaa
      721 ggcttgctga tgattcgaaa gtaattgcta aaataattaa aacagcagac gtagcttcaa
      781 caggaaaaaa agcggcagat gatttaccaa aatttgaaag ggagatggtg gaattagcag
      841 atgaattaga ggtgaaattt ggtaagacaa aaacggcagt ggatggtaca aaaaagcaca
      901 ctgagtttgg gaaacgagta aaagaaatgg ggtatgaaga tgttcaagtg gagcaacctt
      961 atttaggtgg tagaaaaata gaaagtagtg ttgggggttc gagcagacct gatatcacaa
     1021 tagttgatga cgcaggaaca cctacaaatg tatatgatta caaaacagga aatgctaagg
     1081 taagagaatc ccaaagaaga aaaaacgaat caaatactgg cgtcaagcat acaggaactt
     1141 taagagcaac acattaataa attaaactga attcatgaat aaaccagaca ttattaaatt
     1201 cattaaaaaa tatataaacc cgtattttac tgaatacatg caagatagca tttatttact
     1261 ccgagtaagt aaggatcaac tttttatata tggttatatt tttgatagaa attctgcccc
     1321 tgaaaacata attaattgca gggcatttat tgtacctatg ttctatccaa ctgattttat
     1381 ttatttggat tattgttctg aaattaaata cataggtaag ggtaataata ttgacttgaa
     1441 aacgcctatt gatccagaag ttgtaaactg gttaattggt caactaaatg atagaaggat
     1501 atttttcgaa tcttatgttg gagcaaacaa gtttttaaaa tattttgaag attttcattc
     1561 tactttatat aaagatccgg tttcaatttt tgcagcaata cttagcaaca agcaattatt
     1621 tgaacatgat tttgaagtat tttacgcaga ggctcggccg cgaatgaatg atgtcgcatt
     1681 tattgcagag cgatttagaa tcatatctga aattcatcaa ttatataaag ttgcaccgga
     1741 aacggcaaaa aatcatttgt tagcgacagc tgagatttcg tttaaaaaat taaaattaaa
     1801 taaagtttta acgcattctt tagaacatcc aaatggtatt tagatatttg cctcattaag
     1861 aatttagggt taagtgaaat tagtattcta tttacttgtg aaataatagc tttagcaaat
     1921 ttacacgaaa caatcatgga tggtaatgtg acagatttac acaatatatt gacacatttc
     1981 gttaatttag tttttatgca agattagatt tgtaattttg agtgtagcaa ttatttgtaa
     2041 tggttcattt agaaatgctt agatcagatt tagcaatatc atactaaaac taaagtaaaa
     2101 gcagttatta atatgtgaat gaaatgattg ttctgaaaat gggtttcata atcactagaa
     2161 caggtgtggg gcagataact tgataaactt taaatatgta ggtttcatta cttttattta
     2221 atattggttt gtaatgcagg atttaggttt aaaggttatt ttggaattca ataaaatatt
     2281 aaattaatga gagaaattca taacgaatct gctaaatttt atatgtttac taatagatag
     2341 tatgaataat aagtttatgc ttcgagtatt tccttttttg gcgacaggtt tgctaaatgt
     2401 ctttttgatt cttggattgt catatgattt tgcatttgac tatagggtgc atgatgccta
     2461 tttcacatta tcaaatggtc atgcactatt tatttttgca ataattgcca gctattttat
     2521 aacattcatt tggtatgtaa aattcacaat taaacttcat tatttctatt tatggctact
     2581 ttcggcctta atattaattt ttattgttgt tttgccaagt agttttggtt ttgatatcca
     2641 ggaattagaa aacattccca accgcaaaat taaatatttg acatttttca attttattgt
     2701 gcaatttaaa ttatttattt ttttacaact ttgtgtgaat atttatattt tacatgaagt
     2761 agataaaaag tataataata agtcttcagt ttacaaaaat taaaatttga tgtgatgttt
     2821 gtttgacggt aataagtggg tgttattgct attgtgatta ttaaaactta tggaattaac
     2881 tcatgcgcag aaaagaaatt gaatctaaat taataaaaat aatagcttca aatgccgaaa
     2941 atttgggggt ataaattttc atatgaagtt acgggcaaaa taaattcgta ctattttttt
     3001 gtgatagaat cgaaccaatg cctatttaca caatatatac gttttatgaa accaaatttc
     3061 ttaaaatgtg acagcgaaat tttagtttct aataatcaaa tttcatcgtt aattaaaact
     3121 atttcaccta attgtttgat taaaagttac actaatggtg gatatgatat ttctgaaaat
     3181 gagatttttt caattctaaa attcgcttta ggaggttata ttcatcatga ttataaatca
     3241 actggagaac tccaatgcca atggaacagg accaacatac ataatgttat tattgatagg
     3301 gagcctatta atttagaatt aaaatctaaa gaaataattg acaactattt taaaccagta
     3361 attaataacc taatacctca gttaaacagt ttatccaaaa ttgatgctat tttaaataat
     3421 tttaatgaac ttaaattgcc tgttgttgaa ccaccagcct taagtgtttg tttcccatat
     3481 tcacagcaat tagttatggg tctactttta gctaattttt taaatagaaa agataaggtt
     3541 caattgacaa aaaaatattt ggaattggcc tc
//
LOCUS       DAORKW010000384         3555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1404_length_3555_cov_6.301714, whole genome shotgun sequence.
ACCESSION   DAORKW010000384 DAORKW010000000
VERSION     DAORKW010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1404_length_3555_cov_6.301714"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PLB46_12870"
     CDS             complement(<1..394)
                     /locus_tag="PLB46_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF77461.1"
                     /translation="MNKTSQKTALVTGAGSGIGKAICVQLAAAGHRIIAVSRNVEHLA
                     EIESVLNNAAHVMVNVDLTTGVGISTLSGALQQNGMPHIVVNNLSTSSTRKKLIHFAS
                     TISDEIVTENIRHLFAIMPETIAFQREQQ"
     gene            complement(391..1395)
                     /locus_tag="PLB46_12875"
     CDS             complement(391..1395)
                     /locus_tag="PLB46_12875"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HRF77462.1"
                     /translation="MKKGWGVKIIGTGICLPGEPVTTEELSKRLNLEFDIAMVRDKIG
                     IKTRHIAPDDIATSHIAAKAAQMALDNAGITAKQLDRILLGTSTPDYTNTAASCNVQF
                     LLGATCPVGDTTESCASFMYALDHGIRLIATGMQYVCVIGADIKTRFVRKDDPKFCPI
                     FGDGAGAVILTRGDDDSGFMVSELFADGSGLKNIYVPAGGSAMPASIETVTQGLHGTL
                     MTISGKKMVEISIDIMAGIGKKVCDVYGIQPNDVDILIPHQANYIIMKGVAEKLGIDI
                     QKMEVSIDTAANTISGTLPITLHQAVSRNKLQPGKLVLFVTAASGYAGGAAIYKVPSA
                     "
     gene            complement(1397..2446)
                     /locus_tag="PLB46_12880"
     CDS             complement(1397..2446)
                     /locus_tag="PLB46_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HRF77463.1"
                     /translation="MKINGTYFSNLHGVRISGVSTAFPEKKVHNYSIHELLYGKNWSA
                     VFAEKNYDADYIKNVLGYEERYWAHIPGTPIVKNEITSADLMVSASEKVLKENKFATS
                     DIEMLIAVTVTSPKYTNSMGAFVAGKLGLQCPAIEVKTGCASGLYALVMAAQFIRSGA
                     KHVLITAGETPTKVTGINGNLIYAVGDAGAAVILSKDDNAAGISTAFLGTEGAYSGAM
                     GSPGLLPPNQEDLDALAYNMVMGKESETFILEAWQKIPALLYEHSGIYSNDIDLLVPH
                     QVNKKNLSLVKNAAGKNTNQTIDVINQYANCGSVGVLIALHEAYKQKTNNQTKKIMMA
                     AVGGGVSYGGLIVNL"
     gene            complement(2443..2688)
                     /locus_tag="PLB46_12885"
     CDS             complement(2443..2688)
                     /locus_tag="PLB46_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRF77464.1"
                     /translation="MTRTIIIQQITPVFQKVFNDTALTLLPEHSAADFDKWDSINHVI
                     LIARIEKLMKINISTGELIKIKTVGELADLIAEKLAE"
     gene            2897..>3555
                     /locus_tag="PLB46_12890"
     CDS             2897..>3555
                     /locus_tag="PLB46_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HRF77465.1"
                     /translation="MSFNSYIYILFLPLVLTVYYMTGNKYRWLVLLVASYLFYMMYEV
                     AYGLILLTSTTICWVIARQIHITKEKNKRKQLLTIGIIADAGILFVFKYFGFFASIVE
                     SFNHPEALMQAPQTILLPIGISFYTFKSLGYIIDVYRKVTKPEPHFGYFALYVSFFPQ
                     LIAGPIERANELIPQLHQTKKLGYDDLQYGLSRMAWGFFKKLVIADTVAGFVNFTFSY
                     IG"
BASE COUNT         1029 a          706 c          569 g         1251 t
ORIGIN      
        1 attgttgttc gcgctgaaat gcaattgttt caggcataat ggcaaacaga tgacgaatat
       61 tttcagttac aatttcatcg ctgatagttg aagcaaagtg aattaatttt tttcttgttg
      121 aggatgtact taaattattg acaacaatat gcggcattcc attttgttgt aatgcgcctg
      181 ataaagtaga aattccaaca ccggtagtta aatcaacatt taccattaca tgtgcagcgt
      241 tgttcaaaac ggattcaatt tctgcaagat gttcaacatt tctactaact gcaattatgc
      301 gatgacctgc agctgctaat tgcacacaaa tggctttacc tatgccactt cctgcaccgg
      361 taactaatgc tgttttttgt gatgttttgt tcatgctgaa ggtactttat aaatggctgc
      421 accacctgca tatcctgatg cggcggttac aaataaaacg agttttccgg gttgtaattt
      481 atttctacta actgcctgat gtaatgtaat tggtaatgtt cctgaaatag tatttgctgc
      541 tgtatcgata cttacttcca ttttctggat atcaatgcct aatttttcag caactccttt
      601 cataataata taattagcct gatgtggaat taaaatatct acatcattag gctgaatgcc
      661 ataaacatca caaacttttt ttccgatacc tgccataata tctatagata tttccaccat
      721 tttttttccg gaaattgtca tcaatgtacc atgtaatcct tgtgttactg tttctatgga
      781 tgcaggcatt gcacttccac ctgcgggtac ataaatattt tttaatcctg agccatctgc
      841 aaataattca cttaccataa atcctgaatc atcatcgcca cgcgttagaa ttactgcgcc
      901 agcaccgtcg ccaaaaatgg gacaaaattt agggtcgtct tttctgacaa aacgtgtttt
      961 aatatctgcg ccaattacac atacatattg catacctgtt gctatcagtc gaataccatg
     1021 atcaagtgca tacataaaac tcgcgcagct ttcggtggta tcgccaacgg gacaagtagc
     1081 acctaataaa aattgcacat tacaactggc tgctgtattg gtataatcgg gtgtagatgt
     1141 tcctaataaa attctatcta attgttttgc tgtaattccg gcattatcta atgccatttg
     1201 agcagctttt gcagcaatat gacttgttgc aatatcatcg ggtgcaatat gtctggtttt
     1261 aataccaatt ttatcgcgca ccattgcgat atcaaattcc agatttaatc gcttactcag
     1321 ctcttcagtt gtaaccggct ctcccggcaa acaaattcct gttcctatta tcttaactcc
     1381 ccaacccttt ttcatattat aaatttacga ttaatccgcc ataggaaact ccccctccga
     1441 cagcggccat cattattttt tttgtttgat tatttgtctt ttgtttatag gcttcatgta
     1501 gtgcaattaa tacaccaact gaaccacagt ttgcatactg attaattaca tcaatagtct
     1561 gattagtatt ttttcctgct gcatttttaa ccagcgataa gtttttttta tttacctgat
     1621 gtggaaccaa taaatcaata tcattgctat agattccgga atgttcatac aataaagcag
     1681 gtattttttg ccaggcttca agtataaatg tttcactttc ttttcccatc accatattat
     1741 aggcaagtgc atccaaatct tcctggttag gtggtaataa acccggactt cccattgcgc
     1801 cgctataagc cccttcagtc ccaagaaatg ctgtacttat ccccgccgca ttatcatcct
     1861 tcgatagaat aacagcagcc ccggcatcac caacggcata aattaaattg ccatttattc
     1921 cggttacttt agttggtgtt tctcccgctg ttatgagtac atgttttgct ccagagcgaa
     1981 taaattgcgc cgccataacc aatgcatata aacctgaagc acaaccggtt tttacttcta
     2041 ttgccggaca ttgtaatcct aattttccgg caacaaatgc acccatcgaa ttggtgtatt
     2101 ttggcgaagt aacagttacc gcaattaaca tttcaatatc tgaagtggca aatttatttt
     2161 cttttaacac tttttcagac gcgcttacca tgaggtcggc agaagtaatt tcatttttta
     2221 caattggtgt tcccggaata tgtgcccaat aacgctcttc ataacctaaa acatttttta
     2281 tataatctgc atcataattt ttttccgcaa atacggctga ccaattttta ccatataaaa
     2341 gttcatgtat agaataatta tgtacttttt tttcagggaa agcagtactt actccactga
     2401 tgcggactcc atgtaaatta gaaaaatatg ttccgttaat tttcattctg ctaatttttc
     2461 cgcaattaag tcagcaagtt ctcccactgt tttaattttt attaattcgc cggttgaaat
     2521 attaattttc atgagttttt caatacgcgc tattaatata acatgattta tactatccca
     2581 tttatcaaaa tcagcagctg aatgttctgg caaaagggtt aaagcggtat cattaaatac
     2641 cttttggaat acaggggtaa tttgctgtat aattattgtg cgggtcatga ggcaatatta
     2701 aagctaattt caaatacgga ggtagcattt tcatgtgata ttacggtttt atcaaactat
     2761 cttgactttt aatattaaac acatttaaaa tctttatttt attgccttat tgcctatttt
     2821 ttcacaatct tgacacctaa taatttgcat acattatacc tttgagaagt caatttaaag
     2881 tcccattttt accctcatgt cgtttaattc atacatctat atattgtttt tgccattggt
     2941 gctgactgta tattacatga caggaaataa ataccgctgg ttagtgcttt tggtagctag
     3001 ttatctgttt tacatgatgt atgaagtagc ttatggatta atattgctta catcaactac
     3061 catttgttgg gttattgccc gtcaaataca tattaccaaa gaaaaaaaca agcgaaaaca
     3121 attattaact attggtatta tagcagatgc cgggatttta tttgttttca aatactttgg
     3181 gtttttcgcc tctattgttg aatcctttaa tcaccctgaa gcattaatgc aggccccgca
     3241 aactatttta ttacccatag gcatttcatt ttatacattt aagtcgctgg gatatattat
     3301 agatgtatat cgtaaggtaa ccaaacccga acctcacttt ggttattttg ctttatatgt
     3361 ttcatttttc cctcaattaa tagcaggccc tattgaaaga gccaatgagt taatcccaca
     3421 acttcatcaa acaaaaaaat tgggctacga tgatttgcaa tatggtttaa gcagaatggc
     3481 ttgggggttt ttcaaaaaat tagtaatcgc tgatacggtc gccgggtttg tcaatttcac
     3541 tttttcctac atagg
//
LOCUS       DAORKW010000385         3555 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1406_length_3555_cov_3.347143, whole genome shotgun sequence.
ACCESSION   DAORKW010000385 DAORKW010000000
VERSION     DAORKW010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1406_length_3555_cov_3.347143"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="PLB46_12895"
     CDS             complement(<1..970)
                     /locus_tag="PLB46_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF77466.1"
                     /translation="MTFTHLITLKAQYCIDDRFAQSAYFDELEIERDFNIQYGLADDW
                     FMDFPQPLLNTFDIAYPKLDVDPLEKRPFILFAHGGGFWGGEKEALGYHVTQLAEAGF
                     VAASMNYRKGWLGSGDDCTGDPVSLSVAIYRAMQDVQACLRYIVANADTYGIDTAQIY
                     VGGESAGVYALMNGLYMTEEEWSAIHPTHVADYGPLFSSTNDLTNTFTVKGIINMWGG
                     MIDTAYMIDEPMIPMINFYGIADDVIPPTSGPIKFCEGYEVVYGSESMAQVLTNYNVC
                     NVLHQNLFAGHEAYLPEYTSRNIACFVKKQLCESCSTEVLNYALPEC"
     gene            complement(1149..2048)
                     /locus_tag="PLB46_12900"
     CDS             complement(1149..2048)
                     /locus_tag="PLB46_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF77467.1"
                     /translation="MKNQLLLIVFVLVAVVGKTQDTSGLYPINNTELYLEIMGEGEPI
                     LIVHGGPGLNHEYLLPHLAPLAKKHKLIFYDQRSCGKSQLNVKANMNFATFAADIEAI
                     RVFFHIDKLNILCHSWGALPVATFIQNYPEHVKAVAFINPVPFTDEYAMESSRNAASR
                     VSTADSLKRIEIMKTPEFKAGNVKSVSDLMMLSLKQLLCDTANISKIDPKLPDNYMVA
                     SLSLYGFGREMHDYDFYAQIKDNKTPALVIRGACDISPEIADTKIAGCFTNAKLYVFE
                     NSGHFAFVEENKRFVKRVNKFFK"
     gene            2263..2508
                     /locus_tag="PLB46_12905"
     CDS             2263..2508
                     /locus_tag="PLB46_12905"
                     /inference="COORDINATES: protein motif:HMM:NF018958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HRF77468.1"
                     /translation="MLSNNDILKKLRVALSLRNEDIIHILSLVDFEITKGALGDMFRN
                     EDHPNYKQAGDQILRNFLNGLIIYKRGKKEDTPEVAE"
     gene            complement(2776..2931)
                     /locus_tag="PLB46_12910"
     CDS             complement(2776..2931)
                     /locus_tag="PLB46_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77469.1"
                     /translation="MTTQQIADRLVALCRNGQMLEAEQELFADDVVSIEPVYSPAKSA
                     HGKIAVL"
BASE COUNT         1179 a          667 c          640 g         1069 t
ORIGIN      
        1 aacattcagg taaggcataa ttaagaacct cggtgctaca agattcgcat aattgttttt
       61 tcacaaaaca ggcaatatta cgagaggtat attccggtaa atacgcttca tgaccagcaa
      121 ataaattttg gtgcaaaaca ttacaaacgt tgtaatttgt taaaacctga gccattgatt
      181 cagagccata aacaacctca tatccttcac aaaattttat tggtccggaa gtaggcggaa
      241 ttacgtcatc cgcaatacca taaaaattaa tcattggtat cataggttca tcaatcatgt
      301 aagccgtatc aatcatacca ccccacatat taataattcc ttttactgta aatgtattgg
      361 tgagatcgtt ggttgatgaa aacaacgggc cataatctgc aacatgtgtc ggatgaattg
      421 ccgaccattc ttcttcggtc atatataacc cgttcattaa tgcgtaaaca cctgcgcttt
      481 cgccaccaac atagatttgt gcagtatcaa taccataagt atcggcattt gcaacaatgt
      541 atcttaaaca agcctgcaca tcctgcattg cacgatagat ggctacggat aaagaaaccg
      601 ggtcgcctgt gcaatcgtcg cctgaaccta accatccttt gcggtaattc atagaagctg
      661 caacaaaacc ggcttccgcc aattgggtaa catgataacc aagtgcttct ttttcaccac
      721 cccaaaaacc gccaccatgg gcaaataaaa taaatggtcg tttttctaaa ggatctacat
      781 ccagttttgg gtaggcgata tcaaatgtat tgagtaatgg ttgtggaaaa tccatgaacc
      841 aatcgtcggc aaggccatat tgaatattaa aatctctttc aatttccaat tcatcgaaat
      901 acgcagactg cgcaaaacga tcgtcgatac aatactgtgc tttaagggta ataaggtgtg
      961 tgaaggtcat aaggaggatt agaaccttct tcataattgc tgttgggtta agttgaataa
     1021 aaatgctgtg tgggcatttc taaattgggt ataagaacga ttatacaaat ttacaaaccg
     1081 ttttccactc ttttatggca aaaagcagaa attaatgaca ttcaaaacag gatacctaag
     1141 cctggtggtt atttgaaaaa cttattaaca cgctttacaa agcgtttgtt ctcttccaca
     1201 aaagcaaagt ggccggagtt ttcaaataca tacagtttcg cattagtaaa acagcctgca
     1261 attttggtat cagcaatttc agggctgatg tcgcaagccc cacgaataac cagcgcagga
     1321 gttttattat cttttatttg agcataaaaa tcgtaatcat gcatttcacg tccaaaccca
     1381 tacagcgaaa ggcttgcaac catgtaattg tcgggtaact taggatcaat tttggaaata
     1441 ttagcggtat cacataacag ttgttttagt gacaacatca tcaaatcgga tacgcttttc
     1501 acatttcccg ccttaaattc gggggttttc ataatctcga tacgcttcaa actatcggct
     1561 gtcgaaactc tggaagctgc attgcggctt gattccatgg catattcatc ggtaaaagga
     1621 accgggttta taaatgcaac agcttttaca tgttcggggt aattttgaat aaaagtggca
     1681 accggcaaag ccccccagga atgacagaga atattgagtt tatcgatgtg gaaaaacacc
     1741 ctgattgcct caatatcagc ggcaaaagtg gcgaaattca tattcgcttt tacatttaat
     1801 tgtgacttcc cgcaagaacg ctggtcgtaa aatatgagtt tatgcttttt tgccaatggc
     1861 gccaaatgcg gtaatagata ctcatgatta agacccggac caccatggac aataagaata
     1921 ggttctcctt cacccattat ttcgaggtaa agttcagtat tgtttatagg atacaaaccc
     1981 gaagtatcct gcgttttccc aaccacagca acaagaacga atacaattaa gaggagttga
     2041 tttttcatat gcctaagttt tttgcctgtt acaaagttac atgttgttgg gggtggaaag
     2101 aaataccgaa aagcagaatg ttcaatgatt ggtttgaaaa tgacaatcca ctgttaaact
     2161 atagttataa ttattcacat catgagccat cctgctccaa aacaaaacca cgataagacg
     2221 caaaaattat ttattattac gaagaaataa caaaagtcaa acatgttaag taacaacgat
     2281 atattaaaaa aactacgtgt agcgttatcc ttgcgcaatg aagatatcat acacatttta
     2341 tccttagtcg atttcgaaat aacaaaaggc gctctcggcg acatgttccg caacgaagac
     2401 caccccaact acaaacaagc gggagatcag attttgcgta attttttgaa tggactaatt
     2461 atttataagc gcggcaaaaa agaagacact ccggaagtcg ccgaataatt aaactaaaaa
     2521 accgataagc aataaatatt tactgcttat cgatttaaaa atttcgaaaa attggtttta
     2581 gaaaaagaat tgttcgctga tgattttacc atcgcgtact tcgtacaaac gaatttcatc
     2641 aaatgccatg cgaccttgtc ctttgaatgt agcatcgagc gacatggcaa tagtaaaaaa
     2701 tttaccagct actgttggat cagaaataga cccaccgtgg cgttcttcaa ttgaattggc
     2761 aaatgctttt cctttttaaa gtacagcaat tttaccatgt gccgatttag ccggtgaata
     2821 aaccggttca atactaacca catcatctgc gaacaattcc tgttccgctt ctaacatttg
     2881 accattgcgg caaagcgcca ccaaacgatc tgcaatttgt tgagttgtca tatatttcgt
     2941 tttgtatata acgattgagg tggtggggat tggacaacac aaaaaaaaat gtttcggtgg
     3001 gatttaaaag tggggataga ttaattttgg tgcatggagc agctgcatca acaatttcag
     3061 acaaaaggta ttagttattt cgacagaata aaaatgggtt tattatttac aattgtgata
     3121 tgtttaataa tattattttt tggtaattcc agccactgtt agtttattac caaatcaccg
     3181 aactgcctaa ttatcattgg tgctgaaaat gacttaaagt tttttttaaa taattgattc
     3241 attgaagagc ttaggaacct tctgtttttg cttttacggc atttctggag gtcaaataaa
     3301 taatattaat tattcctaaa atgagaatta gttttttgac aatagtaatt aattccgttt
     3361 tttacttatg ggtatttttt gggaaaacgt gtcaaatatt atttaaaact attgacaaac
     3421 acataaattc aattttgaaa ttatatagtt aaccaaacaa caaagccttg tcttttagtc
     3481 tatatcccaa ataattttaa aattacaaaa acctgtttta atggcctttt ttttagacac
     3541 aaaggtgaag gaaac
//
LOCUS       DAORKW010000386         3554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1410_length_3554_cov_4.938554, whole genome shotgun sequence.
ACCESSION   DAORKW010000386 DAORKW010000000
VERSION     DAORKW010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1410_length_3554_cov_4.938554"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLB46_12915"
     CDS             <1..186
                     /locus_tag="PLB46_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77470.1"
                     /translation="YQKGRLWNVNAYYDQNGVILDQGDFCDGTGVVNVYATNGKIIKK
                     KWYKNGRLVKERRVKEN"
     gene            complement(183..950)
                     /locus_tag="PLB46_12920"
     CDS             complement(183..950)
                     /locus_tag="PLB46_12920"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRF77471.1"
                     /translation="MAVTSKNKKNTLVKQYVLTVGEKNFIVFVYLENRYDCRASIGTK
                     GIHIRLSKYMGERERFDQEMSLLDWAKKYIAKHQLHKKPPLFRQYRNGDTLKVMGKEF
                     LIQLVYVPQKNASSGSLKNGVIYLQLSEGMSPKQDQKHKTYLVSKLIGNEFQPQIAQR
                     VQYLNDLHFQKPIKKLRLRDSITNWGSCSWDGNINISVRLLFAPPEVIDYVLIHELAH
                     LVEHNHSARFWKLVEKIMPGYQLAEDWLHKNGEQCIF"
     gene            complement(1038..1724)
                     /locus_tag="PLB46_12925"
     CDS             complement(1038..1724)
                     /locus_tag="PLB46_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77472.1"
                     /translation="MQPLKTLALTLLLIISTGAFAQKELIVTEGSATTSLGERTGFTI
                     TIDKKSEKETLKALKTWIGEMQKKPDFEETVKHEFKLTGFVNASISPDPINVYFLFTE
                     YSDYVKVTGFFEVNLTFVASTTLPDKVEPCKNFMRRFGLRMEKLGVEEQLAAAQKELN
                     KRNDEQASLEKKNKQLNEQIKEWEESIAKAKSDLEQNGKDQESKKSEIAKQKTVNEEI
                     EKKLKEYEGY"
     gene            1950..3332
                     /locus_tag="PLB46_12930"
     CDS             1950..3332
                     /locus_tag="PLB46_12930"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HRF77473.1"
                     /translation="MRKLLYITLLCCSTLGVQAQLEQSSFGATGRGGTATSFVTDYQA
                     LGINPANLGFQTDYAFSLGLAEIGYGFYSEALAKDDVRAIIFNNEDSISQEESQVLAQ
                     SFLNEGLRFDVDVMPIGFSLRVPKLGTFAFSVTAHASYFTKFEGEAANIVFEGYNYAE
                     YFDTVIIDGSEIYGIAYDPVSLSTLFGDSEFKLDINTSINLGYGLKLFGDEETISGYA
                     GIGFKYILGYAYLDMHSDGSTITGLSALGLDILDLEAGITNPITTSAAEPVGRGTGWD
                     FGFSLKVKDNLTASVAITDIGKIKYTANVLQINDYVLDTVSFSGVTTTDPIQIISDML
                     NEQELIAYSGVGAFEVNLPTKLRMGASLKINDMLDIGLDAVFPMNEVAGAFETPVIGA
                     GAQVKLFEILNLSAGISAGGGYAYNIPAGIGLDFKFWELGVATRDALTWFGETSPNVS
                     MALGVLRFKI"
BASE COUNT         1043 a          605 c          678 g         1228 t
ORIGIN      
        1 tatcagaaag gcaggttgtg gaatgtgaat gcctattatg atcaaaatgg tgtaatccta
       61 gatcagggcg atttttgtga tggcacaggt gtggtaaatg tgtatgcaac caacggaaaa
      121 attatcaaaa agaagtggta taaaaacgga cgtttagtaa aagaacgtcg tgttaaagag
      181 aattaaaaaa tgcactgctc accgttttta tgcagccagt cttcggcaag ttgatatccc
      241 ggcattattt tttcaaccaa tttccaaaat cgcgcagagt gattgtgttc tactaaatgc
      301 gctaattcat gaattaatac ataatcaatt acttccggcg gcgcaaataa caatcgtaca
      361 ctaatattaa tgttgccatc ccagctgcaa cttccccaat ttgttatact gtcacgcagc
      421 cgcaattttt ttatgggttt ttgaaaatgt aaatcgttca aatattgcac acgttgcgca
      481 atttgtggct gaaattcatt gccgataagt ttactcacca aatacgtttt gtgtttttgg
      541 tcttgtttcg gcgacatgcc ttctgacaat tgtaaataaa taaccccgtt ttttaaactc
      601 cctgaggagg cgtttttttg aggtacataa acaagttgaa tcaaaaattc tttacccatt
      661 actttcaggg tatcgccgtt tctgtattga cgaaaaagtg gtggtttttt gtgtaattga
      721 tgtttggcaa tatacttttt tgcccaatcc agcaacgaca tctcctggtc gaaacgctca
      781 cgctcaccca tatattttga aagacggata tgaatacctt ttgtgcctat gctggctctg
      841 caatcgtatc tgttttccag gtaaacaaat acaataaaat ttttttcgcc aacagtaagc
      901 acatactgtt ttacaagggt gtttttttta tttttcgatg taaccgccat cagcaataca
      961 aaaataaata tttttttgag ctattgtaaa taaaaaaagc gcctgcaatg cagacgcttc
     1021 taataatatt tcgaaaatta atacccttcg tattctttta attttttctc aatttcttca
     1081 ttcaccgttt tttgtttcgc tatttcagat tttttacttt cttggtcttt gccattttgt
     1141 tctaaatctg attttgcttt tgcaatcgac tcttcccatt ctttaatttg ttcattaagt
     1201 tgtttgttct ttttttccaa agaagcctgt tcgtcgttgc gtttatttaa ttctttttgt
     1261 gccgcagcca attgttcttc aacacctaat ttttccatgc gtaagccaaa tctgcgcata
     1321 aaatttttac aaggttccac cttatcaggg agagttgtag aagctacaaa ggttagattt
     1381 acttcaaaaa atccggtcac tttcacgtag tcggagtatt ctgtaaataa aaaatacaca
     1441 ttaatggggt cggggcttat agatgcattt acaaaaccgg tgagtttaaa ctcgtgtttt
     1501 actgtttctt caaaatccgg ttttttttgc atttcgccta tccatgtttt cagggcttta
     1561 agcgtttctt tttcactttt tttatcaatt gtaatggtaa agcctgtccg ctcacctaaa
     1621 ctagtggtgg cagagccttc ggtaacaatt aattcttttt gagcaaatgc acctgtgctt
     1681 attattaaca ataaggtaag tgccaaggtt tttagaggtt gcatacagtt gttcatttta
     1741 gcgttaaggg tttgtcaaaa accattccaa ttcgtaacat tcccaaataa gcggtaaagt
     1801 tatgctgaaa tgtttttgtt gatgatgaca ttagttgctt aaaagtataa caacaatggt
     1861 aaaatggaca acaatttcaa aaatatgttt ataattggca ttgtgaaaat atagttattg
     1921 ataattttgg atgattataa acaaattaca tgcgtaagtt actctatatc actttattgt
     1981 gctgctcaac attaggtgtg caggcgcagt tggaacaatc ttcatttggt gctacaggac
     2041 gtggtggaac agcaacatca tttgttactg attatcaggc acttggtatc aaccctgcca
     2101 atttgggctt tcaaaccgat tatgcttttt cgttgggttt ggcagaaatt ggttatggat
     2161 tttattcaga agcactggct aaggacgatg taagagctat aatattcaat aatgaggaca
     2221 gtatttcgca ggaagagtct caagtattgg cgcaatcgtt tttaaacgaa ggtttgcgat
     2281 ttgatgtaga tgtgatgcct atcggattca gtttgcgtgt acctaaatta ggaacattcg
     2341 catttagtgt aactgcacat gcatcttact ttaccaaatt tgaaggtgaa gcagcaaata
     2401 ttgtgtttga aggatataat tacgccgaat attttgatac tgtaataatc gacggctccg
     2461 aaatttatgg tattgcgtac gacccagtaa gtttatcgac gttatttggg gattccgagt
     2521 ttaagttgga catcaatact tctattaatc ttggttacgg attaaaattg tttggcgatg
     2581 aggaaacaat tagcggatat gctggtatcg ggtttaaata tattttaggt tatgcctatt
     2641 tagacatgca ttcagatggt tcaaccataa ccggattaag tgcgcttggg ttagacattc
     2701 ttgatcttga agcaggtatt accaatccta tcaccaccag tgctgccgaa ccggttggtc
     2761 gcggaacagg atgggatttt ggatttagtt taaaagtgaa agataattta acagctagtg
     2821 ttgccattac cgatattgga aaaattaaat acacagccaa cgtattacaa attaatgatt
     2881 acgttttaga taccgtttca tttagtggtg ttactactac tgacccaatt cagattatta
     2941 gtgatatgtt gaatgaacag gaattaattg cctattcagg tgttggcgca tttgaagtaa
     3001 atctccccac taaattaaga atgggtgcat cattaaaaat taatgatatg cttgatatag
     3061 ggttagatgc cgtatttcca atgaatgaag tagccggtgc atttgaaaca ccagttattg
     3121 gtgcaggtgc acaagtaaaa ttatttgaga tacttaattt atcggccggt atttctgcag
     3181 gtggtggtta tgcatataat attccagctg gtatcggttt agattttaaa ttctgggaac
     3241 taggagttgc aacccgcgat gctttaacct ggttcggcga aaccagtccg aatgtttcaa
     3301 tggcactggg tgtgttgcga tttaaaatat aaaaattaat tattcagtct ccgaatataa
     3361 aacattggcc tactatcatg agcaggaatt gctgtgcact catatggaaa gaattatcaa
     3421 aaacaagatt taatcaataa taattttttt tgagcgaatt ccattttcat ttttaaaaac
     3481 cacaagatat gctccggcgg caacattttg caaatcaaat tccactgaac cggttttaac
     3541 ttccttattt ataa
//
LOCUS       DAORKW010000387         3552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1412_length_3552_cov_6.340006, whole genome shotgun sequence.
ACCESSION   DAORKW010000387 DAORKW010000000
VERSION     DAORKW010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1412_length_3552_cov_6.340006"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLB46_12935"
     CDS             <1..542
                     /locus_tag="PLB46_12935"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRF77474.1"
                     /translation="EQFSGSNMKNCIFNNAELTALVLAYNNIEKCDFTASDLRGSKIE
                     GSNLLNNQFNKCSFIDAVFLKNNISKNDFSEADFSGAEFINGYFDSNTINDVIWNITS
                     FKNTGFGNITFNGKMQNCHFENCAFHNVKFQNMDITTTFFKYNRKMKKVVFENCKVDK
                     LTYAFLKNNQANLTGLTIV"
     gene            653..880
                     /locus_tag="PLB46_12940"
     CDS             653..880
                     /locus_tag="PLB46_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77475.1"
                     /translation="MKKMTCNQLGGACDLEFHGDTFESIASQSKQHAMEMFQKGDQPH
                     LAAMQKMQEMMQSPTAMQEWMMAKKAEFDLL"
     gene            944..1387
                     /locus_tag="PLB46_12945"
     CDS             944..1387
                     /locus_tag="PLB46_12945"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF77476.1"
                     /translation="MATKNKTTVTTQNVDDFIESFVDNQQKKQDSFQLIKLMQQWSGF
                     EPHMWGPSIIGFGSYHYKYASGHEGDAPMIAFSPRKAAISLYVFVANEENKHLLNNLG
                     KFKMGKACMYIKTLSDIDTNVLKTLCETTIEHLNKNYTCACKTTK"
     gene            1488..2501
                     /locus_tag="PLB46_12950"
     CDS             1488..2501
                     /locus_tag="PLB46_12950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF77477.1"
                     /translation="MKFFVFCLFLTLTSLSFAQNDLKDQFQIFTINNKKLGKVEFCTF
                     KSTINQKKPILIFIHGSGNEPTFAYQPDLKTYAWKGFLEIEQYKDLYHVICVSKPGIP
                     LFDTIQKDPVNFSTTYPLNDTFIQQYSLEWRAEAASLIINQAIKLLPVDQSKIVVIGH
                     SQGGQVAPKVAVLNKKVTHVVMLNTNALNHLYDFTLQERLMSFTGEQTFEQSQQNIDS
                     LFSEYAKIFAEPNSREKLFWNETYYRWASFSDETPLENMLKLKIPIYMVAGGKDIWQS
                     FVMNSDYAQIEFLRHHKTNLTYQVYPNANHFLQDEIVENGKTEYVNIKSQIFKDIHEW
                     TNK"
     gene            2580..3035
                     /locus_tag="PLB46_12955"
     CDS             2580..3035
                     /locus_tag="PLB46_12955"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF77478.1"
                     /translation="MTKEILVTSPDSEIISTRIFQYDKNLVYRAWSEPEHLKNWWGPA
                     GFTNTFHEFDFQVGGKWVFTMHGPDKGNYENACTFIKIEPNALIAWDRISKPLFQVVA
                     TFETIDDGYTLLTFKMLFYSAEDCGKLKPFVVDKNEENFDKLEMVLANM"
     gene            3063..3491
                     /gene="arr"
                     /locus_tag="PLB46_12960"
     CDS             3063..3491
                     /gene="arr"
                     /locus_tag="PLB46_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HRF77479.1"
                     /translation="MAIYDISEMKNAPATYYHGTKAILQIGDLIEPGYVSNYGSRKKA
                     NYVYFTATLDAAIWGAELAVGEGSESIYIVEPSGNFEDDPNLTDKKFPGNPTKSYRTK
                     SALKVVGVVEKWEGHSPELLKAMKDNLTRLKEQGIEAIED"
BASE COUNT         1268 a          592 c          607 g         1085 t
ORIGIN      
        1 aagaacagtt tagtggttcc aacatgaaaa attgtatatt taacaatgct gaattaacgg
       61 cactcgtttt agcctacaac aatattgaaa aatgtgattt tacggcttct gacctacgag
      121 gtagcaaaat agaaggctct aacctgctaa ataatcaatt caacaaatgc tcatttatag
      181 atgctgtttt tttgaaaaac aatatcagta aaaacgattt ttcagaggcg gatttttcag
      241 gggcagaatt cattaatggg tattttgaca gtaacacgat taacgatgta atttggaata
      301 tcacttcctt taaaaatacc gggttcggca atataacatt taatggaaaa atgcaaaatt
      361 gtcattttga aaattgtgcc tttcataatg tgaagtttca aaatatggac ataaccacta
      421 cctttttcaa gtataaccgt aaaatgaaaa aagtagtttt tgaaaattgt aaggtggata
      481 aattaaccta tgcatttttg aaaaacaatc aggccaactt aacgggttta acaatagtgt
      541 aatgtattcg tattaaagtg tattgaaatt gagtttcagc gtttgtatca cttatagtat
      601 tactagttat cacacttttt attaagtttg atgaaatttt aataatacca atatgaaaaa
      661 aatgacctgc aatcaattag gtggtgcttg tgatttggaa tttcacggag acacctttga
      721 atcaattgcc agccaaagca aacagcatgc tatggaaatg tttcaaaaag gtgaccaacc
      781 tcacctagct gccatgcaaa aaatgcagga aatgatgcaa tcgccaacag ccatgcaaga
      841 atggatgatg gcaaagaaag ccgaatttga tttgctttga gcaacctatt gaaatgtatt
      901 tagattcatt ttgttccttg aagtttgtta gttacattag attatggcaa ccaaaaacaa
      961 aacaactgtc acaactcaaa atgtagatga cttcattgaa tcatttgttg ataaccaaca
     1021 aaaaaaacaa gacagttttc agctcattaa gctgatgcag caatggtcag gctttgaacc
     1081 acatatgtgg ggaccttcca taattggatt tggcagttat cattataaat atgccagcgg
     1141 acatgaaggt gatgcgccaa tgatagcttt ttcgccgcgc aaagctgcaa tttccttgta
     1201 tgtttttgtt gcaaacgagg aaaataaaca tctcctaaac aacctgggaa aatttaaaat
     1261 gggtaaagcc tgtatgtaca tcaaaacctt atcggatata gatactaacg ttttaaaaac
     1321 tttgtgcgaa acaactatcg aacacctcaa caaaaattat acttgtgctt gcaaaaccac
     1381 aaaataatgt tttaaccatt ttgttatcag aatatttaaa aagttcaatt acttttaccc
     1441 gtccaacacc taattaataa actgagttaa tttttagcac ctgtagtatg aaattttttg
     1501 tgttttgttt attcctcacg ctgacatccc tatcgtttgc tcaaaacgat ttgaaagacc
     1561 aatttcaaat atttactatc aacaataaaa aattaggtaa agttgaattt tgcactttta
     1621 aatctacaat caatcaaaaa aaacctatac ttatatttat tcacggttcc ggtaatgaac
     1681 caacttttgc ttatcagccg gatctaaaaa cttatgcctg gaaaggattt ttagaaatag
     1741 aacagtataa ggacttatat catgtaattt gtgtttccaa gccaggcatt ccactttttg
     1801 acaccattca aaaagatccg gtaaatttta gtacgactta tccgttaaac gatacattta
     1861 ttcaacaata ttctctggaa tggagagcag aagccgcatc actaataatt aatcaggcta
     1921 taaaactact ccctgttgat caatctaaaa tagtcgtaat aggccattct caaggcggac
     1981 aggtggctcc aaaagtggca gtcctcaata aaaaagtaac gcacgtagta atgttaaata
     2041 caaacgcact aaatcactta tacgatttta cgttgcagga gcgactcatg tcatttactg
     2101 gagaacaaac atttgagcaa agccaacaaa atatcgacag cttattttcc gaatacgcca
     2161 aaatatttgc cgaaccaaat agtcgcgaaa aattattttg gaatgaaacg tattaccgat
     2221 gggctagttt tagtgatgaa accccgctgg agaatatgtt aaaactcaaa atacccatct
     2281 atatggtagc aggtggaaag gatatttggc aatcgtttgt tatgaattct gattatgcgc
     2341 aaattgaatt tttacgtcat cataaaacca atttaactta tcaggtttat ccaaatgcca
     2401 atcatttttt acaagatgaa atagtcgaga atggcaaaac cgaatatgtg aatattaaat
     2461 cgcaaatttt taaagatatt catgagtgga caaataaata agttattata aataaaaaag
     2521 cagtcttaat tttgtgagac attactatat ctttataaga ttacctatta acaaccgtaa
     2581 tgacaaaaga aatcttagtt acatcacccg atagcgaaat tatcagcaca agaatatttc
     2641 aatatgataa aaacctggtg taccgtgctt ggtcagaacc tgaacacctg aaaaactggt
     2701 ggggtcctgc aggatttacc aatactttcc atgaatttga ttttcaggta ggtggtaaat
     2761 gggtatttac tatgcatggt cctgataaag gaaattacga gaatgcctgc acctttatta
     2821 aaattgaacc taatgcactt attgcctggg accgtatttc taaacctttg tttcaagttg
     2881 ttgctacttt cgaaacaata gatgatggtt acaccctgct cacatttaaa atgctatttt
     2941 attcagctga agactgtgga aagttaaaac cgtttgttgt tgataaaaat gaagaaaatt
     3001 tcgataagct ggaaatggtg ttggctaata tgtaaaataa ttattcaaaa ataaatctga
     3061 ttatggcaat ttacgatatt tctgaaatga agaacgctcc tgctacatat tatcatggaa
     3121 caaaagcgat tctccaaatt ggcgatttaa ttgagcccgg atacgtatcg aattatggca
     3181 gtagaaaaaa agccaattat gtctatttta ctgcaaccct cgatgcagct atttggggcg
     3241 ccgaactagc tgttggagaa ggttccgaaa gtatttatat cgtggaacca tcaggaaatt
     3301 ttgaagacga ccctaacctt accgacaaaa agtttccggg aaatccaaca aagtcatatc
     3361 gtactaaaag tgctttaaaa gttgtaggcg tagtcgaaaa atgggaaggt cattccccgg
     3421 aattattgaa agctatgaaa gataacctga cccgtttaaa agaacagggt attgaagcaa
     3481 ttgaggatta attaaaataa ttagaatact acaatatttc aatatccaac aattatcttt
     3541 attgttgaat at
//
LOCUS       DAORKW010000388         3547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1413_length_3547_cov_5.978522, whole genome shotgun sequence.
ACCESSION   DAORKW010000388 DAORKW010000000
VERSION     DAORKW010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1413_length_3547_cov_5.978522"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLB46_12965"
     CDS             complement(<1..440)
                     /locus_tag="PLB46_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77480.1"
                     /translation="MFPFSRNSENPYQQLIAKGLIENGMEVITIPNRKLFPIIQVLKY
                     QFDILYLFWPEDFYVGKNVLTALIKQTSLLLSLPILRNKITIYSADNLFSHTVTPKTV
                     HTESKWINRILKNCSGIIFSSEAAKTIYQNTHKYTPKNYAIVPHV"
     gene            complement(463..1761)
                     /locus_tag="PLB46_12970"
     CDS             complement(463..1761)
                     /locus_tag="PLB46_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77481.1"
                     /translation="MTIAIIVSFFDLRSDLRRLIAELSKTNKVILFVKPEHEALINRH
                     LSNEISYRLINEAKPTLGNFLTTRLFLLFKKLPKSKRNYYLMETFKAHGLTNSKTKKK
                     ALSILFLQKLIPHFMSYDFYLNNLRYTSKTDLSGIDAALTFTEIHDDYLYSRLIKSKI
                     PLFTYVYSWDHAFKHVRFSKRIQYLVWHAGISEDLVTMHGIPNNNITVIGSTQLCYLD
                     QFLSTNTSPKHNYKYFYYACGIGIPSHVEDELGIVIQLAKTIHSIYPTYKLIVRPYPN
                     LKNWNIYDLLKNQTNIILEDNYRDQDLVTSEEQIMEKFRVISNAKGFFHMGTTLGLEA
                     CFTKCPVFIIATVPKSQYSVSIYDFVYQSQNVKYLIHSSKLNTIASYNQLRETLINID
                     DPIFLQFSNLIKSQFNTESFELITTKFTNYLSSYNTHHAQ"
     gene            complement(1767..3269)
                     /locus_tag="PLB46_12975"
     CDS             complement(1767..3269)
                     /locus_tag="PLB46_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77482.1"
                     /translation="MTIKTNHFFKNISSNYFIYAAKLAIGLYALPVCLAAYGSEIYGL
                     YLLSFGLSSALAAFDLGSSRSIYRYTIEFGSDNNLEKFKTALDTSYSFNLFSAILTAT
                     LIIILGQFSTTLFNLPHDSKQTATTIFLLAAINTFITMLDAAPQSLITANNLFHARNK
                     YIGVSILLNLIIILAVQYSSAINIELYAFLVTITSIFVWFMDHELVKSKNLLKGIKVS
                     FKNLSYLKRSAYADYSLQVFGLSLISFMAIQADKLIIAIVLDTSAVTLYTIITKPYFV
                     FKGILATTIPVVQPKLNEYYFNNLSNNFIQLSRKFTRISFFIMLCCTILIGLYYKDLL
                     SIWLKTNTYNTYANWGIISMATLCLTLFYTQFLRTLLHTNKIKYIIYFSIIAVSINVI
                     TSSILSYYIGFQGVIIGTTVQITLELIYYKYLERKFVSIDKPKPSGYNYLIYIFTIIL
                     LSVVCFIMLEYFITSYLLKIVSFIVLLSAFSIVTYMILIKENIMKRATLH"
     gene            complement(3287..>3547)
                     /locus_tag="PLB46_12980"
     CDS             complement(3287..>3547)
                     /locus_tag="PLB46_12980"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRF77483.1"
                     /translation="VYQGAGFKGKLAAAMEICAKENITLNEVAYIGDDINCIELLKAV
                     GLAACPANSTKKVKAIPGIIHLQKSGGEGAIREFVDDYILIE"
BASE COUNT         1199 a          506 c          593 g         1249 t
ORIGIN      
        1 acatgcggaa cgatagcata gtttttaggg gtatacttat gggtgttttg gtaaattgtt
       61 tttgccgcct cgcttgaaaa tattattccg gagcaatttt ttaatatacg gtttatccac
      121 ttggattctg tatggactgt tttaggcgtg acggtgtggc taaacagatt gtctgcagaa
      181 tatatggtta ttttatttct taaaataggt aaacttaaaa gcagggaagt ttgtttaatt
      241 aatgcggtaa gcacattttt gccaacataa aaatcttcag gccaaaaaag atataaaata
      301 tcgaattgat attttaggac ctgaattatg ggaaataatt ttctatttgg gatagttatc
      361 acctccattc cattttcaat taacccttta gcaattaatt gttgatatgg gttttcacta
      421 ttccttgaaa agggaaacat aagcactctt atttttttac cctcactgtg catggtgtgt
      481 attataacta cttaggtaat tcgtaaactt tgttgtaatc aattcaaacg attcggtatt
      541 aaattgggat ttgatcaaat tgctaaattg taaaaatata ggatcatcaa tattgattag
      601 ggtttctctt aactggttgt aacttgcaat tgtatttagt ttacttgaat gaattaagta
      661 ttttacattt tgagattgat aaacaaaatc atagatgctt acactatatt gcgatttcgg
      721 aacagttgca attataaaaa cagggcattt tgtaaaacat gcttccaggc ccaatgttgt
      781 tcccatatga aaaaaacctt tagcgtttga aatcacacga aatttttcca ttatttgttc
      841 ctcgcttgta accaggtctt gatccctata attatcttct aatattatat tagtttgatt
      901 ttttagcaaa tcataaatat tccaattctt caaatttggg taaggcctta caattaactt
      961 gtaggtgggg taaatggaat gtattgtttt tgctaattga attacgatac caagttcgtc
     1021 ttcaacatga gaaggaatgc caataccgca tgcgtaataa aagtatttat agttgtgttt
     1081 cggggatgta tttgtgctca aaaactgatc taaataacac aattgagttg acccaataac
     1141 agtaatgtta ttgtttggaa tgccatgcat ggtcaccaga tcttctgaaa ttccggcatg
     1201 ccaaactaaa tattgaatac gctttgaaaa tcttacatgt ttaaatgcat ggtcccaact
     1261 ataaacatat gtaaacagcg gtattttaga ttttattaat cgactataaa ggtaatcatc
     1321 atgtatttct gtaaatgtta gtgctgcatc aattccagat aggtcagttt tgctcgtata
     1381 ccttaggttg tttaaataaa agtcataact cataaaatgt ggtatgagtt tttgtaaaaa
     1441 taaaatcgat aatgcctttt ttttagtttt agaatttgtt aatccatggg ctttgaaagt
     1501 ttccatgaga taataattcc gttttgattt ggggagtttt ttaaataata aaaacagtct
     1561 tgtggtcaaa aaattgccta aagtcggttt ggcttcgttt attagacgat acgatatttc
     1621 attactcaaa tgtcgattga ttaatgcctc atgctcaggt ttaacaaata aaattacttt
     1681 atttgttttg cttaattcgg ctattaaccg tcttaagtca cttcttaaat caaaaaagga
     1741 tactataatt gctatagtca tttttattaa tgcaaagttg cccttttcat aatattttct
     1801 ttaattaaaa tcatatatgt aacaatggaa aaggcactta ataatacaat gaaagaaaca
     1861 atttttaaca aatatgaggt gatgaaatat tctaacataa taaagcagac aactgaaagc
     1921 agaattatcg taaaaatata aataagataa ttgtaaccac taggtttcgg tttgtcaatg
     1981 cttacaaact tgcgttccag gtatttataa tatattaatt ctaatgtaat ttggactgta
     2041 gttccaataa ttacaccttg gaatccgata tagtaggata aaattgaact cgttatgaca
     2101 ttaatagaaa cagctattat tgaaaagtaa ataatgtatt ttattttgtt tgtatgtaac
     2161 aaggttctaa gaaattgtgt ataaaacaat gttaaacaaa gggtagccat gctaattata
     2221 ccccaattgg cataggtatt ataggtattg gttttaagcc atatggacaa caaatctttg
     2281 taatatagcc caatcaatat ggtgcagcat aacattatga aaaaactaat tctagtgaat
     2341 ttgcgtgata gctgtatgaa attgtttgaa agattgttaa agtaatattc attcaatttt
     2401 ggctggacaa ctggaattgt tgtagcaagt atacctttaa aaacaaaata gggttttgta
     2461 attattgtat ataaggttac agcgctggta tccaaaacaa tagcaataat caacttatca
     2521 gcttgaattg ccataaaact tattaaggaa agtccaaaaa cttgaagaga ataatcagca
     2581 tatgctgaac gttttaaata gcttaaattt ttgaaagaaa ctttaattcc tttaagaaga
     2641 tttttacttt taactaactc atggtccatg aaccaaacaa atattgaagt tatggttacc
     2701 aaaaaggcat ataattcgat gtttattgct gaagaatatt gaacggctaa aataattatt
     2761 aaattaagga gtattgaaac ccctatatat ttgtttcgag cgtgaaataa attatttgca
     2821 gttatcaaac tttggggtgc ggcatccagc attgtaataa atgtgttgat tgcagctaga
     2881 aggaatattg ttgttgcagt ttgttttgaa tcgtggggca agttaaataa cgtagtgcta
     2941 aattgaccga gtataattat aagtgttgct gttaaaatag ctgaaaacaa attaaaggaa
     3001 tagctcgtgt ctaaagctgt tttgaatttt tctaaattgt tatccgaacc gaattcaata
     3061 gtatatctat aaatggatct tgacgaccca agatcaaaag ctgctaatgc agaacttaac
     3121 ccaaagctta ataggtataa accatagatc tcacttccgt aagcagctaa acaaacaggt
     3181 aaagcatata ggccaatggc taatttggct gcatatatga aataattgct gcttatgttt
     3241 ttaaagaaat gatttgtttt tattgtcaca acttgatatt gtggttttat tcaattaaaa
     3301 tataatcatc aacaaattcc cgtatagcac cttcgccacc acttttttgc aggtgaatta
     3361 ttcccgggat agcttttact tttttggtgg aattggcagg acaagcggct aaaccaactg
     3421 cttttaataa ctcaatgcaa ttaatatcat caccaatata agccacttca ttaagggtga
     3481 tattttcttt tgcacaaatt tccattgcag ctgctagttt acccttaaag cctgcacctt
     3541 gatatac
//
LOCUS       DAORKW010000389         3547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1414_length_3547_cov_5.663803, whole genome shotgun sequence.
ACCESSION   DAORKW010000389 DAORKW010000000
VERSION     DAORKW010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1414_length_3547_cov_5.663803"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PLB46_12985"
     CDS             complement(<1..966)
                     /locus_tag="PLB46_12985"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF77484.1"
                     /translation="MRNQLLFLLFSASTLFFISCDKKSGDPFTISGTITGAENKKIIL
                     ETMTFPEAGQPKYTIIDTVTADKEGKFTIKNHLPERMTCRLTVAGADQNYYVVSLQNE
                     EMELTANVNEAGNPSITGSPATLSLFGLMKTLRDFDESAMRMNDSIMELKAAGNDSAF
                     NAIVEQMKNDYISIFKNYIDTCKYVANSSIAVESIFFSEFQFVKEHYNVWKSSADSSS
                     PYIKDLGIKIATQEGLLAQSLVGKPFIDIIQPDKNGNTKKLSDLKGQIVLIDFWASWC
                     GPCRKENPNVVRVYNAYKSKGFNIFSVSLDTDKSKWLAAVQADGLV"
     gene            complement(1004..1621)
                     /locus_tag="PLB46_12990"
     CDS             complement(1004..1621)
                     /locus_tag="PLB46_12990"
                     /inference="COORDINATES: protein motif:HMM:NF014930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77485.1"
                     /translation="MRSGLVASSYLCRWMNLYDKYEVVIGLEVRAQLLSKSKACNDAI
                     ATDGGEPNTYVSSVTLGDPGTLPVFNYESWGLGIKLGLTTNSKIVRFNQFASKNYLYT
                     DLPKGYQINHDTAPVSTCDYVVIGAYGIAKTVSFQGTQMQGDGGQSIQDSDLLYTLVD
                     FNRAGTPTSVKKGLLGLLLGEEMKLSPKKANPKLPGKLLEDKFNN"
     gene            1808..3082
                     /locus_tag="PLB46_12995"
     CDS             1808..3082
                     /locus_tag="PLB46_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77486.1"
                     /translation="MKLLYTTLGIALFTTLNAQNFDYAVSLQEVVFSGDDMYEIRQDD
                     NTGYIDTDFHWKADGTVNPTAFPSGTTISAAATIQFECEQMPASIYIRGTGPDDINFP
                     SVAVAPAGDIFNYPETPASAPFEPETIRFYEHFTINWEFSFDNIKWYTIGESMSPVYV
                     TLQQAKPEGAFSGFKYYLTVIDVACRNADGVTTTDEMINKVWEEFTDHTVYRADGVPM
                     GYYKELFSPNVTLGTLIKYADGECYTWAQLFLAALKMHGFSQPNNYMNITADYSSTGC
                     GSISRFLVKDWTFGTPSYDCFDMPYVNVYSPDYYDADTAYIYDYAEVTDEIGVMGQTA
                     PNPASSFSNHQIAIVNGKYYDASYGVLYNTFSEIKYGALSGWSRSETSDEVGIGIDVN
                     GDGDLDASPDFTILRATDDIEAADFDQSIETW"
     gene            3112..>3547
                     /locus_tag="PLB46_13000"
     CDS             3112..>3547
                     /locus_tag="PLB46_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77487.1"
                     /translation="MIAVNSFGQGKFDRKIIIKDSLFYAIEINNQLGVLWTGDVNKPL
                     DSAQQYILPVGTKRRSIHWQPLAWDISGERLYAINFTEFSQNEIISNLKEISLDSLEK
                     YEPETNYRDYFYKAAMENNHVENYPFVETYRDYLYMDNFFFDI"
BASE COUNT         1084 a          622 c          671 g         1170 t
ORIGIN      
        1 aactaacccg tcagcttgaa cagcagccaa ccatttcgat ttatctgtat ctaaagaaac
       61 actgaaaata ttaaatcctt tggatttata agcattataa acacgtacaa cattcggatt
      121 ttctttgcga catggtccac accagcttgc ccagaaatcg attaatacta tctgtccttt
      181 taaatctgat aattttttag tgttgccatt tttatctggt tgaataatat ctataaaagg
      241 tttgccaacc aggctttgtg caagtagtcc ttcttgtgtg gcaattttta tacctaaatc
      301 tttaatataa ggacttgaag aatcagcact gctcttccaa acattataat gctcttttac
      361 aaattgaaat tcagagaaaa atattgattc tactgcaatt gaagagttgg ctacgtattt
      421 acaagtgtcg atataatttt tgaatataga gatataatca tttttcattt gttcgactat
      481 ggcattaaat gccgaatcat ttccggcagc ttttaattcc atgatagagt cattcatgcg
      541 catcgcagac tcgtcaaaat cacgtaaggt tttcattaac ccaaataatg ataatgttgc
      601 aggtgagccg gtaatacttg gattgcctgc ttcatttaca ttcgccgtta attccatctc
      661 ctcgttttgt aacgatacca cataataatt ttgatcggca cctgctactg ttaaacgaca
      721 agtcatacgc tcgggtaaat gatttttgat ggtaaactta ccttctttat ctgctgtaac
      781 ggtatctata atagtgtatt ttggttgacc tgcttccggg aatgtcatcg tttccaaaat
      841 aatttttttg ttttctgcac cggtaatggt gccggatatt gtaaatggat cacctgattt
      901 tttatcacaa gaaataaaga ataaagttga tgcactaaat aataaaaata atagttgatt
      961 tcgcattgct agttaatttg atacttttta aaagtggtat atattaattg ttgaatttat
     1021 cttctaataa tttaccggga agttttggat tggctttttt tgggctcaac ttcatttctt
     1081 cacccaataa aagtcctaat aatccttttt ttacactagt aggagttcct gctcgattga
     1141 agtctaccaa tgtataaaga aggtctgaat cttggatact ttgaccgcca tccccctgca
     1201 tttgagtgcc ttggaaacta actgtttttg ctattccata cgcgccaatc actacataat
     1261 cacaagtgct aacgggcgca gtatcatgat ttatttggta acctttgggc aaatcggtgt
     1321 ataagtagtt ttttgaggca aactgattga atcgtactat tttggaattt gtggtcaaac
     1381 caagttttat acctaaaccc caactttcgt agttgaaaac aggtaaagtg cccggatcac
     1441 ctagtgtaac agagcttaca taagtattcg gttcaccgcc atctgtggct atagcatcgt
     1501 tgcaggcctt ggatttggaa agcaattgag cacgtacttc taatccaatt actacttcgt
     1561 atttatcgta aaggttcatc cagcggcaaa gataagaact tgctaccaag cctgagcgca
     1621 aacagcagcg tttactaaaa tttagtagtg tcagttaaat gtgaaaaaaa tacaaccggt
     1681 tatcagaatt tcaggaattt gttttaactt cgaactgcaa aagattgatt tcttttcata
     1741 tgtatattac ccggtggaca gaagaggagc tggacaccgg tttttttatt tatataagtt
     1801 gctaattatg aaactgttat atacaaccct aggtattgct ctttttacca ccctcaacgc
     1861 ccaaaatttc gattatgctg tctcccttca ggaggtagtg ttttccggcg acgatatgta
     1921 tgagataagg caggacgata atacgggtta tatcgatact gattttcact ggaaagctga
     1981 cggcactgta aatccaactg catttccgtc cggaaccaca atttcagctg cagcaacaat
     2041 tcaatttgag tgcgaacaaa tgccggccag tatatatata cgcggaacag gccctgatga
     2101 tatcaatttt ccaagcgtgg ccgttgctcc tgcaggtgat atttttaatt acccggaaac
     2161 acccgcttct gctccgtttg aacctgaaac gatcaggttt tatgaacatt ttaccatcaa
     2221 ctgggaattc tcatttgata atattaaatg gtataccatt ggggaaagta tgagtcctgt
     2281 atatgtgaca ttacagcagg ctaaaccgga aggagcattt tccggattta agtattacct
     2341 caccgttatt gatgttgcat gcaggaatgc agatggtgtt accactacag atgaaatgat
     2401 taataaggta tgggaagaat ttactgacca cacggtatat cgtgcagatg gtgtaccgat
     2461 gggttattac aaagaattat tttcgcctaa tgtaacacta ggcactttaa ttaaatatgc
     2521 cgatggcgaa tgttatacat gggcgcaatt atttttagcc gctttaaaaa tgcatggttt
     2581 ttcgcaaccc aataattata tgaatattac ggctgattat tcctctacag ggtgtggtag
     2641 tatttcaaga tttttagtga aagactggac atttggaaca ccatcatacg actgtttcga
     2701 catgccgtat gtaaatgtat atagtccgga ttattatgat gcagatactg cctatatcta
     2761 cgattatgca gaagttaccg atgaaattgg tgtaatggga caaaccgctc caaatcctgc
     2821 atccagcttt agtaatcatc aaatagcaat tgtaaatgga aaatattatg atgccagtta
     2881 cggcgtttta tataatacat ttagtgaaat aaaatatgga gctttaagtg gatggagcag
     2941 aagtgaaaca agtgatgaag taggtattgg cattgatgta aatggagatg gtgatttaga
     3001 cgcctcaccg gactttacca ttttgcgtgc aaccgatgat attgaagctg cagattttga
     3061 ccaatctatt gaaacatggt aaaacgattt gtctttaaca ttattttttt aatgattgct
     3121 gttaatagtt ttggtcaggg aaaatttgac cgaaaaatta ttattaagga cagtttgttt
     3181 tatgctattg aaattaataa tcaattaggt gttttgtgga cgggtgatgt aaataaacca
     3241 ttagatagtg ctcaacaata tatccttcct gttggcacaa aaaggcggag tatacattgg
     3301 caaccattag cctgggacat aagcggtgaa aggctttacg cgattaattt tacagagttt
     3361 agtcaaaacg aaatcatttc taatttaaaa gaaatttcac ttgattcact tgaaaaatat
     3421 gaacctgaga ctaattaccg tgattatttt tacaaggctg caatggaaaa taatcatgta
     3481 gagaattatc cgtttgtgga aacctaccgc gattatttat acatggataa tttctttttt
     3541 gatatcg
//
LOCUS       DAORKW010000390         3547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1415_length_3547_cov_5.123998, whole genome shotgun sequence.
ACCESSION   DAORKW010000390 DAORKW010000000
VERSION     DAORKW010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1415_length_3547_cov_5.123998"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLB46_13005"
     CDS             complement(<1..686)
                     /locus_tag="PLB46_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRF77488.1"
                     /translation="MRKLLIGFTALGFCLAFFSTKSKSQTPDFMMQGWYWDYPLFYTG
                     IGNQIDYLNQEGEHLKDAGFTYMWLPPLAKSISGSSSMGYNVRDYYDIGDYGPARWGD
                     REGFEALKTTYNSLGIKMVADMIYNHRDGGSWEDNGAVEGWIENMNYTKVAAGDQPFP
                     SDRYQCYLPIGGATGNGAGTYYFKIRSASEHPNFYSKPYQVIFWTNTVAADYVTAPTT
                     EAEPNGGGDCG"
     gene            730..2148
                     /locus_tag="PLB46_13010"
     CDS             730..2148
                     /locus_tag="PLB46_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF77489.1"
                     /translation="MLNDTRKVVRKTTLLSHCCLFLVVFLVFSAQLIAQMTIRVTSIP
                     QYFMPVLDSVFIAGTFNSWNPGDTMFALEKDFDGDYVINLSGTNGEEIEFKFTRGDWE
                     RGETTLEGGFVANRVEIFENGVEKEYTIANWEDQIGTHTIAGDVIQLDYDFVIPQLSR
                     TRRIWMYLPPDYYTSGISYPVVYLHDGQNVFDLATSFISEWDVDGTMQTLIAAGHTQA
                     IVVVVANGEADRIDEYSPWNHPTYGGGDGDLYADFIVNTLKPYIDANYNTLPDRLNTV
                     IGGSSLGGLISYYMALEHDEIFGKALIFSPSFWFDDSVNIFTQQFEKTLPSKIYITAG
                     LNEDEDMVPDITTITDELQLKGFNEDELITIIRSDGAHSEWFWKREYDDGYLWLFEDL
                     LPLTTENQAHQIKCYYDRKSNTIQFEGIENFDFVICDVMGRIRLNGLANNYINCHTLP
                     KGAYIFTIKSNQLNYTFKFMVL"
     gene            2145..3293
                     /locus_tag="PLB46_13015"
     CDS             2145..3293
                     /locus_tag="PLB46_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF77490.1"
                     /translation="MMQQLKYLMLLSSIICSSYTFSQFTIIVENANDFHDSITNKMFI
                     AGNFNGWNPADSSTMLVWENGVLQTTIVIDSTWSALEYKFTCGSWEKGEVKKNGAPQP
                     NRTCFYKPGMVVEEKIESFAHIAPKWQPAENTSVIKIKLYAETLNKEKTIRILLPCDY
                     EKNDLNYPVLYMLDGQNLFDETMSHSGEWGVDEAMDSLCNIHKMQAIIIGIDHAGEER
                     IAEYSPYPIQDYVTQPQGDAFAEFVVNTLKPFIDSSYRTQPEREFTGIAGSSMGGVMS
                     MYMVMKYNNVFSKAGVFSPAFWTNESNYENAKTIVVQQPTQIYFLAGALEGNEFEVVN
                     DTKKMLDILLQQNNPNINTRFVTQSTGRHTESFWNAEFFEMFEWLFLK"
BASE COUNT         1159 a          572 c          745 g         1071 t
ORIGIN      
        1 ccacaatcgc caccgccatt aggttcagct tcagttgttg gtgctgtgac ataatcagcg
       61 gcaacggtat tggtccaaaa aattacctga taaggtttgc tgtaaaaatt aggatgttca
      121 ctggcggaac gaattttaaa ataataagtg cccgcaccgt tacccgttgc cccgccaatt
      181 ggtaaataac attgatacct gtcgctggga aatggctgat cgccggctgc aactttggta
      241 taattcatat tctcaatcca accttcaacg gcaccattgt cttcccaact tccgccatcg
      301 cgatgattat aaatcatatc agctaccatc ttgattccca gactgttgta agttgttttt
      361 aaggcttcaa acccttctct gtcgccccaa cgtgcaggtc cgtaatcgcc aatatcatag
      421 taatcacgca cattatatcc catgcttgac gaacccgata tcgattttgc caatggtggt
      481 aaccacatat aggtaaatcc tgcatctttt aaatgttctc cttcctgatt caaataatca
      541 atttggttgc caatgcctgt gtaaaataat gggtagtccc aataccagcc ttgcatcatg
      601 aaatctggcg tttgactttt agattttgta gaaaaaaaag ctaaacaaaa accaagtgca
      661 gtaaaaccaa tcaatagctt cctcatataa cctgactata tttgttgcag tagacaaaaa
      721 tacatgaata tgttaaacga tacgcggaaa gtggtaagga aaacaacctt gttgagtcat
      781 tgttgcctct ttttagttgt ttttcttgtg tttagcgcgc aacttattgc acaaatgaca
      841 ataagggtaa cttccatacc tcaatatttt atgccggtac tggattcggt atttattgca
      901 ggcactttta attcgtggaa tccgggtgat accatgtttg ctttagaaaa agattttgat
      961 ggtgattatg ttattaattt atcaggaaca aacggtgaag agattgaatt taaatttaca
     1021 cgcggtgatt gggaaagagg tgaaacaaca ttggaaggtg gttttgttgc aaatagggtt
     1081 gaaatatttg aaaatggtgt tgaaaaagaa tacactattg ccaactggga agaccaaatt
     1141 ggtacgcata ccatcgccgg agatgtaatc cagttagact atgattttgt gattcctcaa
     1201 ttatcaagaa cccgccgaat ttggatgtat ttgcctcctg attattacac ttccggaatt
     1261 agttatccgg ttgtttattt acacgacggc caaaatgttt ttgaccttgc aactagtttt
     1321 atcagtgaat gggatgttga cggcaccatg caaactttaa tcgcagcagg tcatacacaa
     1381 gctattgttg ttgttgttgc aaatggagaa gcagatagaa ttgatgagta ttcgccgtgg
     1441 aatcatccta catatggtgg cggtgacggc gatttatatg ccgactttat tgtgaatact
     1501 ttaaagccat atatagatgc aaattataac acattacccg acagattaaa tacggtaatt
     1561 ggaggaagta gtttgggcgg actaatatca tattatatgg cattagaaca tgatgaaata
     1621 tttggcaagg ccttaatttt ttcacctagt ttttggtttg atgattcagt aaatatattt
     1681 acgcagcaat ttgaaaaaac attgccatct aaaatttata tcactgcggg attaaatgaa
     1741 gatgaggata tggttcctga tattacaaca ataacagatg aattgcaatt aaaaggattt
     1801 aacgaagatg agctgataac aataattagg tcagatgggg cgcacagcga atggttttgg
     1861 aaacgtgagt acgatgatgg ctatttatgg ttgtttgaag acctgcttcc actaacaaca
     1921 gagaaccagg cacatcaaat aaagtgttat tatgatagga aatctaatac aatacagttt
     1981 gaaggtattg aaaattttga ctttgttatt tgtgatgtaa tgggcagaat ccgtttaaat
     2041 ggtttggcaa ataattatat aaattgccat acgcttccta aaggtgcata tatttttacc
     2101 ataaaaagta atcagctcaa ctatacattt aaattcatgg tattatgatg caacaattaa
     2161 aatatttaat gctgctatcg agcattattt gttcaagtta tacatttagt cagttcacaa
     2221 taattgtaga aaatgcgaac gatttccacg attcaattac taataaaatg tttattgccg
     2281 gtaattttaa tggatggaat cccgcagaca gcagtactat gttggtttgg gagaatggtg
     2341 tgttgcagac aacaattgtt attgattcaa catggagtgc actcgaatat aaatttactt
     2401 gtggcagttg ggaaaaaggt gaagtgaaaa aaaacggcgc accgcaaccc aatagaacct
     2461 gcttttacaa accgggaatg gtggtggaag aaaaaattga aagctttgca catattgcac
     2521 caaaatggca accggcagaa aatacttctg taattaaaat aaaattgtac gcagaaacat
     2581 taaacaaaga aaaaaccatt cgcatattat tgccatgtga ttatgagaaa aatgatttga
     2641 attatccggt tttatatatg ttggatggac aaaatttatt tgatgaaacg atgtcgcatt
     2701 ccggtgaatg gggtgtggat gaagcaatgg attcgttgtg taacatacat aaaatgcagg
     2761 ctattataat tggaatagat catgcgggag aagagcgaat tgcagaatat tcaccctatc
     2821 caattcaaga ttatgttaca caacctcagg gtgatgcttt tgctgagttt gtagtaaata
     2881 cattaaaacc atttatcgac agtagttata ggacccaacc ggaacgcgaa tttacgggca
     2941 ttgcaggaag tagtatgggt ggagttatga gtatgtatat ggtaatgaaa tacaacaatg
     3001 tgttttcaaa ggccggtgta ttttctccgg cgttttggac aaatgaaagt aattatgaaa
     3061 atgcaaaaac aattgtcgta caacaaccaa cacaaattta ttttttagca ggtgcattag
     3121 aaggtaatga atttgaagta gtgaatgata ctaaaaaaat gttggatatt ttattgcaac
     3181 aaaacaaccc aaacataaat acacgtttcg taacacaaag cacaggccgc cacaccgagt
     3241 cgttttggaa tgccgaattt tttgaaatgt ttgagtggtt atttttaaaa tgattgtggt
     3301 aattttgttg aaatgatttt tgtagttagt gtttttgccg ggaagtcggg gagaaaggaa
     3361 gtgacgggag gtaaaaatga ttaattatga aaaaatatga attaacagaa agagaacagt
     3421 ttttagcaaa aaaaattgta gatgcggcta ttttagttca caaagaactg gggccggggt
     3481 tactggaaaa agtcgtgatg gtgcagagcg aggaatcggc tcggttcgac gggatgccgc
     3541 gagcggc
//
LOCUS       DAORKW010000391         3543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1419_length_3543_cov_4.754874, whole genome shotgun sequence.
ACCESSION   DAORKW010000391 DAORKW010000000
VERSION     DAORKW010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1419_length_3543_cov_4.754874"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1357)
                     /locus_tag="PLB46_13020"
     CDS             complement(191..1357)
                     /locus_tag="PLB46_13020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF77491.1"
                     /translation="MIWTIIFWICVFCLVHSYVIYPFILRIFGGDLDAPTPLPHPAPQ
                     PMVSVLMAVYNEEKVLEQKIRSVFNTAYPAGQLEMLIGSDGSTDNTDIIIEKLIKEGY
                     RIHFTRFGGRNGKTQIINALAPQANGKIFIPTDANIIFDPLTIPKLVAHFTDEKIGLV
                     AANIINTGLRKDGISYQEHTYIKRENLIKHYEGLLWGTMMGAFGACYAIRANLFPVIP
                     RNFFMEDFYITLKVIQGGKKAIAEPDAIAIEDVSNEITEEFKRKIRISAGNFQNLGVF
                     WPMLLKFYTGAGFSFLSHKVLRWLGPLFLIGAFISSIILFNTNTFYQLAFYLQLAGLL
                     TPLFDKLLSAVNLHIYLLRLLAYFYTMNLALFLGFIKYAKGIKSSTWRPTERNV"
     gene            complement(1354..2604)
                     /locus_tag="PLB46_13025"
     CDS             complement(1354..2604)
                     /locus_tag="PLB46_13025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRF77492.1"
                     /translation="MQKLPLQPKKSIRYLYSPMHILLLSKKNPYPERDGEAIAIMQLA
                     KGLAQNGCKVSILYMNTQKHHFPVSEIPSELTQLINFYAVTVNNAITPIGVLQNLFVS
                     TPYHVQRFFSSPYLSALRQLISNNHFDIIQAEGLYLTQYFNLIPKSANTRFVYRSHNI
                     ESEIWGNIAANTSNIFKRWYLQIQTKKLAHYEKTIPQNIDAIVPISIADFSFYQQITT
                     TIPLHHTPTGIELDQSPIVSAVDFHSLYFLGGLDWLPNIEGLEWFIHEIFPKIIKILP
                     PTTLHIAGRNGDERWKQLHHPQIKYHGEVENAQKFAADKYICIVPLLSGSGMKIKIIE
                     AMHLGKAVVTTPKGAKGMPTGTELHFYTAADAQQFADTVIELLQSQDKVLQKTKLAQQ
                     FVDDTLSHLTITQSLIHFYQKISA"
     gene            2878..3492
                     /locus_tag="PLB46_13030"
     CDS             2878..3492
                     /locus_tag="PLB46_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77493.1"
                     /translation="MLNFLTETTEKVISLLAGAFIVAAQITLVADVLRKKLKPSLLSW
                     VGWALLMGTGLIAQVLEMGWEWSQTGLFFSAFGCLCIVFSALIRKHYLLVKSDFSYLF
                     LGLICLGIYFISKDAWITTVFAILADFIVGIPTLKHAYKNPLSQKSNAWLLGFIAFTF
                     SLIICIGHSLLYALFPIYLFLYNGAMLVLTHRYFVNKKSDSEVI"
BASE COUNT         1089 a          629 c          659 g         1166 t
ORIGIN      
        1 gcatgctgat aattcgtgtt atcgttttgt gcttgaaggg ttgttagcat taaccctgag
       61 agaaacaggc atactatttt cataaaaggc ttttgttgat aacggccatt ttaaaggaat
      121 attacttttc cgcctaaaat ttgaccattc ttaactattg tatcctccac ataagcttga
      181 ggaacagttt tcaaacatta cgttcagttg gtctccacgt gctcgacttg atgcctttag
      241 catactttat aaacccgaga aataaagcca ggttcatggt gtagaaatac gccaataagc
      301 gtaataaata aatatgcaaa ttgacagcac ttagtaattt gtcaaataaa ggggttagta
      361 aaccagccaa ttgcaaataa aatgctaatt gataaaatgt attagtatta aataaaatta
      421 tactcgaaat aaatgcgcca atcaaaaaca aagggcctaa ccaacgtaat actttatgcg
      481 ataaaaaact aaatcccgct ccggtataaa attttaacaa cataggccaa aatacaccga
      541 gattttggaa attacctgca gagattctga ttttgcgttt aaactcttct gtaatttcat
      601 tacttacatc ttcaatagca atagcatccg gttcggcaat ggctttttta ccaccttgaa
      661 ttacttttaa agtgatataa aaatcttcca tgaaaaaatt acgcggtata acaggaaata
      721 aattagctct gattgcataa caagcaccaa aggcgcccat catagttccc cacaataaac
      781 cttcatagtg ttttattaaa ttttcacgtt taatataagt gtgttcctga taagaaatgc
      841 catcttttct taatccggta ttgataatat tcgcagccac caatccaatt ttttcatccg
      901 taaaatgcgc caccagtttt ggaatggtaa gtgggtcgaa aataatattg gcatcggtgg
      961 gaataaatat tttaccattt gcttgcggag ctaaagcatt aataatttgt gtttttccat
     1021 tgcgaccacc aaaacgggta aagtgaatgc gataaccttc tttaattaat ttttcaataa
     1081 taatatccgt gttatccgtt gatccatctg aaccaatcag catttccagt tgacctgccg
     1141 gataggccgt attaaataca gagcgaattt tttgctccag gactttttcc tcattatata
     1201 cagccattaa tacacttacc attggttgag gtgccggatg aggtaatgga gtaggtgcat
     1261 ctaaatcgcc accaaaaatg cgcagaataa atggatatat tacataacta tgcaccagac
     1321 aaaaaacaca aatccaaaaa ataatcgtcc agatcatgcg cttatttttt gataaaagtg
     1381 aattaatgac tgcgtaatgg ttaaatgact taatgtgtca tctacaaatt gttgtgcaag
     1441 ttttgttttt tgcaaaacct tgtcctgcga ttgtaataat tcaattactg tatcggcaaa
     1501 ttgttgtgca tctgctgcag tataaaaatg caattcagtg cctgtgggca tacctttggc
     1561 accttttggt gtggtgacaa ccgctttgcc taaatgcatg gcttcaataa ttttgatttt
     1621 catgcctgag cccgataaaa ggggtacaat acaaatatat ttatccgccg caaatttttg
     1681 tgcattttct acttcaccat gatatttaat ttgtgggtga tgtaattgtt tccagcgctc
     1741 atcaccattg cgacccgcaa tatgtaatgt ggtgggaggt aatattttta ttattttcgg
     1801 aaaaatttca tgaataaacc attccaaacc ttcaatgttt ggcaaccagt ccaaaccacc
     1861 taaaaaataa agtgagtgaa aatcaactgc tgaaacaatc ggcgactggt ctaattcaat
     1921 tcctgttggg gtgtgatgta gaggaattgt tgtggtaatt tgctgataaa aactaaaatc
     1981 ggcaattgaa atcggcacta tggcatctat attttgtgga atagtttttt catagtgtgc
     2041 taattttttt gtttgaattt gcaaatacca tcttttaaaa atgttcgacg tatttgctgc
     2101 aatgttcccc caaatttccg attcaatatt atgacttcta tacacaaatc gagtattggc
     2161 tgatttggga attaaattaa aatattgtgt taaataaagc ccttctgcct gaataatatc
     2221 aaagtggttg ttggaaatta attgacgtaa agcggataaa taaggactac taaaaaatcg
     2281 ctgcacatga taaggagtgc tgacaaataa attttgtaaa acacctattg gtgtaatagc
     2341 attatttacg gttaccgcat aaaaattaat gagttgagtc agctcgcttg gaatttcaga
     2401 caccggaaaa tggtgttttt gggtattcat gtataaaatg ctcactttgc atccattttg
     2461 agcgaggcct tttgcaagct gcataatggc tatagcctcc ccgtcccgct cgggatatgg
     2521 gttctttttg gagaggagta aaatgtgcat agggctgtaa aggtaacgaa ttgatttttt
     2581 cggttgcagg ggcagttttt gcaattgtca catattaatt tcgaattcaa tccacacatt
     2641 cacaggcttt ttctactgcg gcggacgttt cgagaatttg tatatcaaca aaatataaca
     2701 tgttgcgcaa ccatagtcga cacgtgggtc gacggctaca cgccgaaatc aattccacgc
     2761 aatcataggc tgtttccgtc tcagcggacg gttcgcattg gtacatcggt acattggtac
     2821 attttcacat taataagcac atcaccacat cagcacatta actaccttta accccgcatg
     2881 ctcaattttt taaccgaaac caccgaaaag gtaatcagcc tgttagccgg cgcattcatt
     2941 gttgctgcgc aaattacgtt ggttgcagac gtattgcgaa aaaaactgaa gcctagtctg
     3001 ttgagttggg ttggctgggc attattaatg ggcaccggtt taattgcaca ggttttggaa
     3061 atgggctggg agtggagcca aaccggtttg ttttttagcg catttggatg tttgtgtatt
     3121 gtattttcag ctttaatacg gaaacattat ttactggtga aatccgattt tagttatttg
     3181 tttttgggat taatttgttt aggcatttat tttatttcca aagacgcatg gataactact
     3241 gtatttgcca tactcgccga ttttattgtt ggtattccaa ctttaaaaca tgcatataaa
     3301 aatccgttat cacaaaaaag taatgcgtgg ttgttgggat ttattgcatt tactttttcg
     3361 ttaataatat gtataggaca ttcattattg tatgcattgt ttccgattta tttattttta
     3421 tataacggtg ccatgttagt gttaacgcat cgttattttg taaataaaaa atcagatagt
     3481 gaagttattt aactaattaa atcgataggc agcccgtcct gattgccgta tagtgacctc
     3541 att
//
LOCUS       DAORKW010000392         3542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1423_length_3542_cov_2.809005, whole genome shotgun sequence.
ACCESSION   DAORKW010000392 DAORKW010000000
VERSION     DAORKW010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1423_length_3542_cov_2.809005"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLB46_13035"
     CDS             <1..489
                     /locus_tag="PLB46_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77494.1"
                     /translation="EQRREELTYYIGYKIPLAKLDDQLKSLKIQADMDGMLWDWKDKG
                     YSAVLDATEDVEGTKCFKVDVTTDSNDVYTFYIDTESYLLIKSHAKVFMSGQAVESDT
                     YYSNYKDVDGMILPGQIENRYNGVTGELIVIDRYEVDTPYNQSDFNKPVVAETPVTPE
                     NK"
     gene            587..>3542
                     /locus_tag="PLB46_13040"
     CDS             587..>3542
                     /locus_tag="PLB46_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77495.1"
                     /translation="MILFSKFKKQVIFSLFGLSLTGMYAQNAITDATFSVIEGRHIGP
                     ARTSGRIAAIDASDKDPNLVYVGAAGGGLWKSKNQGTTFKPIFDDYTQSIGTICIDQQ
                     HPDTVWVGTGEPWTRNSVSIGTGIYKTVDGGEKFEFMGLAKSERISKIIVDPSNSNTV
                     YVAVQGALWSDSEERGVYKTTDGGKTWSRLLYINPSTGCASLAMDPNNPQVLYAAMWD
                     HRRTAYDFRSGGPGSGLYKSTDGGANWTKLSAGLPTGTIGRIAVAVSKVAPYSVYALV
                     ESEKSALYKSVDNGANWLKMSDQNAMGERPFYFSNISPDPVEAERIYKPGFVLLVSTD
                     GGKIFQGASTEGGAYHSDTHAFYVSPKDNRFMYLGTDGGVYVSRDKGNTWSHCENLPV
                     AQFYHVSVDMQKPYNVYGGLQDNGSWKAPSSNPGGIQNADWETIGIGDGFNAFADKTD
                     PNLVYWQYQGGRVYRSNTKTGENKFIKPFADASTEDLRYNWNTPVHFGKKSDWLYIGA
                     QYLYRSKDKGDSWERISGDLTTDNPLRQQQEKSGGVSADNTTAENNTTIFSISESALD
                     ANVIWVGTDDGNVQVTTDGGKTWSLVSTNIKGLPPLVFISGIESDNFDKAAAYVTVDA
                     HRNGDMQPYIFYTNDYGKTWKSLVNETVKGYCHVVKQDLVNSNLLFLGTEFGLYVSLD
                     KGVTWVQFKSNIPQVGVFDMVIHPRDHDLVLATHGRGIIIIDDITPIRNFKMEMLEAD
                     VTFLPTRPYYLSSGGIVQDFPGDDEFVGNNPNGSAVVAYFMKKRHMIGEMYIEIYDNK
                     GNYIKKQPATTRKGLNIVRIQTMLPPPRVPSSPNPLFEAAFGPELEAGDYLIKLIKGK
                     DTLSTKLTINNNPDVNHSAADRQLRRESLLKAYNLLEELAYVDYQVMEIRKQTLALAD
                     SVKNKGLRANSFKYADKMAVMHEKISATQPGEGGVAGQIRLREKIAEVYSAIGSYEGK
                     PTDLQLAALSVYV"
BASE COUNT         1109 a          648 c          835 g          950 t
ORIGIN      
        1 gaacagagga gagaagagtt gacgtattat ataggttaca aaatacctct agctaaacta
       61 gatgaccaat tgaaatcgct caaaattcaa gccgatatgg atggtatgtt atgggattgg
      121 aaagataaag gctatagtgc agtattagat gcaactgaag atgttgaagg cactaaatgt
      181 tttaaagttg acgtaactac tgattcaaat gatgtatata ccttttatat tgacacggaa
      241 agctacctgc tgattaaatc gcacgcaaag gtatttatga gtggtcaggc tgttgaaagt
      301 gatacctatt atagcaacta taaagatgta gatggtatga tattacctgg ccaaattgaa
      361 aaccgttaca atggtgttac cggagaactc atcgtaatag accgttacga agtagatacc
      421 ccttacaatc aaagtgattt caataaacct gttgttgccg aaactccggt aactccagaa
      481 aataaataat cgacaaatta gtccaaaaag agataccata caggtgtctc ttttttttat
      541 aaataattgt ctatctgtta attatacgat ttaactacta tactatatga tattattttc
      601 aaaattcaaa aaacaggtaa tattcagctt gttcggttta tcgctgacag gcatgtatgc
      661 acaaaatgcc attaccgatg ccacgttcag tgtgattgaa ggtcgccata ttggtccggc
      721 aagaacgagt gggcgtattg ctgctattga tgcttctgac aaggacccaa atctggttta
      781 tgtcggggct gcaggcggag gattatggaa atcgaaaaac cagggcacta cgttcaaacc
      841 gatttttgat gattacacac aaagtatcgg aaccatttgt attgaccagc aacatcctga
      901 cactgtttgg gtagggactg gtgagccttg gaccaggaat agtgtgagta taggaacagg
      961 tatctacaaa accgtagatg gtggcgaaaa atttgaattt atgggtcttg ccaaaagcga
     1021 gcgtatttcc aaaataatag tcgacccaag caatagcaat accgtatatg ttgcggtgca
     1081 gggagcatta tggtccgaca gtgaagaaag aggagtttac aagactaccg atggtggtaa
     1141 aacatggagt agattacttt atataaatcc ttcgacagga tgcgcgagcc tcgccatgga
     1201 tcccaacaat ccgcaagtat tatatgccgc catgtgggat catcgcagaa cagcttatga
     1261 tttccgttca ggtggtccgg gaagtggatt gtataagtcg acagacggag gtgccaactg
     1321 gacgaaatta agtgcaggat tgccaacagg aaccattgga aggatagccg tagcggtatc
     1381 aaaagttgct ccttatagcg tttatgcctt ggttgaatcg gaaaaatctg cattatataa
     1441 gtctgtagat aacggtgcta actggttgaa aatgagcgac caaaacgcaa tgggtgaacg
     1501 tccattttac tttagtaata tatcacctga cccggttgaa gccgagcgca tttacaaacc
     1561 cggctttgta ttattggtga gtacagatgg aggtaaaata ttccagggag catcaactga
     1621 aggtggtgct taccatagcg atacccatgc tttttatgtg agtccaaagg acaatcgttt
     1681 tatgtatttg gggactgacg gaggagttta tgtttcccgt gacaagggca atacctggag
     1741 tcattgtgaa aatttaccgg tagcgcaatt ttaccatgtg agtgtggaca tgcaaaagcc
     1801 ctacaatgtg tatggcggat tacaagataa tggcagttgg aaggccccta gcagcaatcc
     1861 gggaggcatt cagaacgccg attgggaaac tatcggtata ggagatggat ttaacgcctt
     1921 tgcggacaaa accgatccta atttagttta ttggcaatat cagggcggac gcgtttatcg
     1981 ttcgaacact aaaaccggtg aaaacaagtt tataaaaccc tttgcagacg cttctacaga
     2041 agacttacgc tacaattgga atacaccagt gcatttcggt aaaaaatcgg attggttata
     2101 tattggtgcg caatatttgt atcgttcaaa agataaagga gacagctggg aacgtatatc
     2161 cggtgattta actacggata acccgctcag acaacagcag gaaaaaagtg gtggagtgag
     2221 tgccgacaat acgacagcgg aaaacaatac taccatattc agtatcagcg aatctgcatt
     2281 ggatgctaat gtgatttggg ttggaacaga tgatggcaac gtgcaggtaa cgacagatgg
     2341 cggtaaaacc tggagcctgg taagcacaaa tattaaaggt ctgccaccgc tggtgtttat
     2401 ctcaggaatc gagtcagata actttgataa agcagcagct tacgtaactg tagatgccca
     2461 tcgcaatggc gatatgcaac cttatatatt ctacaccaat gattacggta agacatggaa
     2521 aagccttgta aatgaaacag ttaagggata ttgccatgtg gttaaacaag atttagtcaa
     2581 ttcaaatttg ctctttttag ggactgagtt tgggttgtat gtgagcctgg acaaaggcgt
     2641 tacctgggtt caatttaaga gtaatatacc gcaagttggc gtgtttgata tggtgattca
     2701 tcctcgcgac catgacttgg ttttggcgac acacggaaga ggaattatca tcatcgacga
     2761 tattacacct attcgcaact ttaagatgga gatgctggaa gcagatgtta cctttttacc
     2821 taccagacca tattacttat cttcaggagg tatagttcag gattttcctg gcgatgatga
     2881 gtttgtaggt aacaacccta atggtagtgc agtagttgcc tatttcatga aaaagcgcca
     2941 tatgattggt gaaatgtata tagagattta tgacaataag gggaattaca tcaaaaaaca
     3001 acctgccacc acacgcaagg ggttgaacat cgttcgtatt caaactatgt tgccaccgcc
     3061 aagggtgccc agctccccta acccattgtt tgaagccgct tttgggccag aactagaagc
     3121 cggtgattac ctgatcaaac tgatcaaagg caaggataca ctaagtacca aacttactat
     3181 taataacaat cccgatgtta accatagtgc tgcagacaga caattgcgcc gggagtcgtt
     3241 gttaaaggct tataatttgt tggaagaact agcctatgtt gattatcagg ttatggaaat
     3301 ccgcaagcag actttagcgt tggccgacag tgttaaaaac aaagggttga gagccaattc
     3361 gttcaaatat gccgataaaa tggctgttat gcatgaaaaa atatctgcga cccaacccgg
     3421 tgaaggcggg gttgccggtc aaatacgttt gcgtgaaaaa attgctgaag tgtattctgc
     3481 aattggcagt tacgaaggta aaccaaccga cttgcaatta gcggcacttt cggtatatgt
     3541 tg
//
LOCUS       DAORKW010000393         3540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1424_length_3540_cov_4.421234, whole genome shotgun sequence.
ACCESSION   DAORKW010000393 DAORKW010000000
VERSION     DAORKW010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1424_length_3540_cov_4.421234"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PLB46_13045"
     CDS             complement(<1..1229)
                     /locus_tag="PLB46_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77496.1"
                     /translation="MQNNKSILNHLLPLTLLIIALISIWGYTIGHGNSIQLLPYLQYV
                     HDETLFANDFFIQNAIQNLPNERSFFIAILQPFATNPEWPVFILFVLTTYLLLYALLQ
                     IANSFLMDYRLSYLVLCILLFPLLYHTLGLNEIYFGELNANYVADAFSAWAIVFVLRK
                     KIWLSYIMMILATLMHPLAGFHTFLLITGALIIQAIIARNIKQVLTNYLRPIVSYCFT
                     AGVYIVFLQIRLHDAEWDEASFFQAFFVFRNAHHYIPTAFSTLDWKILTPLYLLPLFV
                     FYKRNNLLFIITALIIIGCVVYSFIVLNYHSPAVATGQWFKTTIWLELFGVISIVLLL
                     QMLLQIINKTQLTTAVLILFLSGIAAWTFLKFPGIQKIKTDITYELPFYRKMTPELDI
                     AIKAGAVTDKDALFIDEL"
     gene            complement(1232..2527)
                     /locus_tag="PLB46_13050"
     CDS             complement(1232..2527)
                     /locus_tag="PLB46_13050"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRF77497.1"
                     /translation="MNNTPKKISGTNAIQLFQIARYGALILTSVLLTKTGIDTSVIGH
                     YENFILLAGTLSFFWVNGLTHTFLSVYNQHENKRKVIGQTAIIVIVMSICVALGMLIF
                     RTPLTTLFAFDDTGNVFHLLLCYFIFNNISFLLDYMLLARNNVKGLLWLSAYHLVFQT
                     ALIALPAFYFGSFETIITGLLIFTGCKFIITVFFLLRNNEYQPDKAFIIMYLQKAAPL
                     IMAFFLGGMSIYVDGIIVNNYYDKATFATYQYGAREFPLSLLLANALSASMILKIGQD
                     NTAFGEVKKASLTLIHRLFPVGIVLLIVSQWVYPVVFNATFSESFIYFNIYMLLLVPR
                     LLFPHSILLGLNQQKTILKASVVEFTLNIILSLTLLQFMGLQGIAFGTVIAFVVNKTI
                     LLITLNKQGIPPSAYIPLKQISTYSVILVIVFILFTYVV"
     gene            complement(2531..>3540)
                     /locus_tag="PLB46_13055"
     CDS             complement(2531..>3540)
                     /locus_tag="PLB46_13055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRF77498.1"
                     /translation="IAVLVIACPCAMGLATPTAIMVGLGRSARNGILIKGATTAEQFA
                     KTSIVVFDKTGTITTGDFKVTNFTAFESNTEVVSGIIYALEKHSSHPIAVSLIKHFTP
                     NADVQLKNITEQKGVGMFGEDEQGNKYQLISGTAINNVFDIILLRNEDLMAGFNIRDE
                     IKDDAEAAIQYFKSKGIKTVLLSGDNSTKVNEVGGKLSFDIVRSNQQPAEKLIFIEDL
                     KKEGLVTMVGDGINDSPALEKADVGISMSNASQIAINSAQIILLNGKLNKLISAHQLS
                     EQTLRTIKQNLFWAFFYNVIAIPFAAVGLLNPMIAALSMAFSDVFVIGNSILLKTKKL
                     R"
BASE COUNT         1219 a          624 c          639 g         1058 t
ORIGIN      
        1 agttcatcaa taaacaaggc atctttatcg gtaactgcac cggctttgat ggcaatatcc
       61 agttccggtg tcattttacg gtagaagggt agttcgtagg taatatctgt ttttattttt
      121 tgaatgcccg gaaattttaa aaaggtccag gcggctatgc ctgataaaaa taggataagg
      181 actgctgtag tcaattgtgt tttgttaatt atttgcaata acatttgtaa aagcaataca
      241 atacttataa cgccaaataa ttcgagccaa atggtggttt taaaccattg ccctgtggca
      301 acagcaggag agtggtagtt taaaacaata aaactatata ccacacaacc aataatgata
      361 agcgcagtta tgataaacaa caaattattg cgtttataaa aaacaaataa gggtaaaaga
      421 tataatgggg tgagtatttt ccaatccaaa gtactgaaag cggttgggat ataatgatgc
      481 gcatttctaa acacaaaaaa ggcctggaaa aaagaggctt catcccattc tgcatcatgc
      541 aggcgtattt gaaggaatac gatataaacg ccggcagtaa agcaatacga cacaattggc
      601 ctcagataat tggtaagcac ctgtttaata ttacgtgcga tgattgcttg tatgattaat
      661 gcaccggtaa tgagcaaaaa ggtatgaaac cctgcaagcg gatgcatgag tgttgccaat
      721 atcatcataa tataactcag ccatattttt ttgcgcaaca caaaaactat agcccaggca
      781 gaaaaagcgt ctgcgacata attagcattg agttcaccaa aataaatttc atttaatcca
      841 agggtatgat acaataaggg aaataataaa atacataata ctaaataact cagtctgtaa
      901 tccattaaaa atgaattcgc aatttgcagc aatgcatata ataacaaata ggtggttaat
      961 acaaataaaa taaaaacagg ccattccgga ttagtagcaa atggttgtaa aattgcgatg
     1021 aaaaacgaac gttcgttggg gagattttgg atggcattct gaataaaaaa atcgttggca
     1081 aacaaggttt catcatgcac atattgcagg taaggcaaca attgtatact attgccatga
     1141 ccgatggtat aaccccaaat gcttatcagg gctattatta gcaaggttaa tggcagtaaa
     1201 tggtttaata tcgatttatt gttttgcatg cctacactac ataagtaaat aaaataaata
     1261 cgatgactaa aataacacta taggtgctta tttgtttcaa cggaatataa gcggaaggtg
     1321 gtattccttg tttattaagc gtaatcagta aaattgtttt gttgaccaca aatgcaatta
     1381 cggttccgaa ggcaataccc tgcaatccca taaattgtaa taacgtaagg ctcagtataa
     1441 tatttaaggt aaattcgaca acagaagcct tcaaaattgt tttttgctga tttaatccca
     1501 acaaaatact atgcggaaat aataaacggg gaactaacaa taacatataa atattgaaat
     1561 aaataaaact ctccgaaaaa gtggcattaa atactaccgg atacacccat tgacttacaa
     1621 tcaataaaac gatacccacc ggaaataagc gatgaattaa tgtgagcgat gcctttttta
     1681 cttcgccaaa tgccgtattg tcctgcccga tttttaatat catagatgca cttaatgcat
     1741 ttgccaacag caatgacaac gggaattcac gcgcgccata ctgataagta gcaaatgtgg
     1801 ctttgtcgta ataattattt acaataatac catccacgta aatcgacatg cctcctaaaa
     1861 aaaatgccat gattagtgga gctgcttttt gtaaatacat gatgataaac gctttgtcgg
     1921 gttggtattc gttatttcgc aacaaaaaga aaacggtaat gataaattta catcctgtaa
     1981 atattaataa tcccgttata atggtttcga aactgccaaa ataaaatgca ggcagtgcaa
     2041 ttagggcagt ctgaaacacc agatggtagg cactcaacca caacaaccct tttacattat
     2101 tgcgggctaa taacatgtag tccaacaaaa aggaaatgtt attaaaaata aaataacaga
     2161 gcaataaatg aaacacattt cccgtatcat cgaatgcaaa caatgttgtt aagggtgtgc
     2221 gaaaaatgag catacctaag gcaacgcaaa tggacatgac tattacaata atggccgttt
     2281 gaccaattac cttgcgtttg ttttcatgct gattatacac cgataaaaaa gtatgtgtaa
     2341 ggccatttac ccaaaaaaaa ctcagggtgc ctgccaacaa aataaaattc tcataatgtc
     2401 cgattactga tgtgtcgata cctgttttag taagtaaaac agaagtgagt attaatgctc
     2461 cgtatctggc aatttgaaat aattggattg cgttggttcc cgatattttt tttggtgtgt
     2521 tgttcacgtt ttagcgtaat tttttggttt tgagcaaaat ggaattgcca attacaaaaa
     2581 catcggaaaa tgccatcgac aatgctgcaa tcatagggtt gagcagtccg acagcggcaa
     2641 aaggtatggc tatgacgttg taaaaaaacg cccaaaataa attttgttta atagtgcgca
     2701 aggtttgttc acttaactgg tgtgcagaaa ttaacttatt taatttgcca tttaataaaa
     2761 taatttgtgc tgaattaata gcaatttgcg aggcattact cattgaaatc cccacatcag
     2821 ctttttccag tgcaggggag tcgttaatgc catcgccaac catcgttacc agaccttcct
     2881 tttttaaatc ttcaataaat attaattttt ctgccggttg ctgattactt cttacgatat
     2941 caaaactcaa tttgccacct acttcattta ctttcgtgct gttatctcca ctgagtaaaa
     3001 ctgtttttat ccctttagat ttaaaatatt gaatagcggc ttctgcatca tctttaattt
     3061 catcacgaat attaaaacct gccattaggt cttcattgcg taataaaata atgtcaaata
     3121 cattgttgat ggcagttccg gaaattaatt gatatttatt accctgttca tcttccccaa
     3181 acatacccac acctttttgt tcagtgatat tttttaactg aacatccgcg ttgggagtaa
     3241 aatgtttgat taatgaaact gctatgggat gtgatgaatg tttttcgagt gcataaataa
     3301 ttccactcac cacttcagta tttgattcga atgcagtaaa attggtcacc ttaaaatcac
     3361 ctgtggttat ggtgcctgtt ttatcgaaaa caacaatgga agtttttgca aattgttctg
     3421 cagttgttgc acctttaatt aatataccat tacgtgcaga gcggccaagg ccaaccatta
     3481 tggcagttgg tgttgctaaa cccatagcac atgggcatgc gataaccagt actgcaatac
//
LOCUS       DAORKW010000394         3538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1425_length_3538_cov_4.879989, whole genome shotgun sequence.
ACCESSION   DAORKW010000394 DAORKW010000000
VERSION     DAORKW010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1425_length_3538_cov_4.879989"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLB46_13060"
     CDS             <1..1131
                     /locus_tag="PLB46_13060"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRF77499.1"
                     /translation="QGRDIIDLSLGEPDFETPEHIKAAAKKAIDDGFTHYTPVAGYLD
                     VRKAIAEKFMRENGLLYTPEQIVISTGAKQSIINAVLCLVNPGDEVILPTPFWVSYSA
                     MVKLAEGKIVEIPTSIESDFKITPEQLEKAITPKTKLIMFSSPCNPTGTVYTKDELKA
                     LAEVVARHENIYIISDEIYEHINFTGQHASMAQFDFIYDRVITVNGLSKAYAMTGWRL
                     GYIGAPLWIAKACDKIQGQYTSATCSITQRATIAALEGDVETTLTMKAAFLRRRDLVF
                     QMMQNIKGLKFNNPQGAFYFFPDVSNYFGKTYNGNTINNADDMAMYLLNEGNLSVVTG
                     SAFGDQNCIRFSYATSDDKLVEAMKRLKIALEKLITQEIPVS"
     gene            1138..2133
                     /locus_tag="PLB46_13065"
     CDS             1138..2133
                     /locus_tag="PLB46_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HRF77500.1"
                     /translation="MANAAAIKKMFSGFRHIHVAIIGDIMVDKYVYGNVDRISPEAPV
                     PVLDVKQKETRPGGAANVAVNIKALDATPYLFSVIGNESEGDNLIGLLKLKGINTKHI
                     IRSEKRITTAKTRIISKNHQMMRIDEEDISDLDESLSKKLLQSILKFLQNTPPDVCIF
                     EDYDKGVLTPEMISKIIHICNQLNIPVIVDPKKKNFFAYQHCTVFKPNIREINESLHT
                     SLQYVDLTSLNNITTLLHQQLTHKITLITLGDKGIYLRAGKKSSIQKAHVRNVADVSG
                     AGDTVVSVAALCLAAGAEPSQMAAIANIAGGLVCESPGVVSIDKSQLIQECIDLL"
     gene            complement(2142..3410)
                     /locus_tag="PLB46_13070"
     CDS             complement(2142..3410)
                     /locus_tag="PLB46_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF77501.1"
                     /translation="MSVFFRTLSETFPQVMQELRNNKLRTFLSLFGVSIGIFCIIAVL
                     SAVDSLKRNMESGLDKLGTNMIYIQRWPWQFNNEEYAWWDYIKRPTMSYEEYKLLNEK
                     LTKAKAVAVEVWLTADNVKYLDNDLQNVAISGVTENYDEIYTLNFKGGRYFVPNESVG
                     GSEVAIIGFEVASQLFPPNIDPVGKYIWFNVSGRQYKMKVIGVLEKEGESIIDISNDN
                     SIIIPFNYIRRVVDMKSGFIDPVIEVRPKDGITADEIKDEIYPIMRNARNLRPTEASD
                     FAVNEVTLAADALDSLFGMLNIVGFIIGGFSILVGGFGIANIMFVSVKERTNVIGIKK
                     SLGAKNYLILTEFLIEAIVLALIGCLFGLALVWLVMFAADKATGFGFILSWSNVLIGT
                     SISVALGVIAGIIPAITASRMNPVEAIRSK"
BASE COUNT         1136 a          723 c          628 g         1051 t
ORIGIN      
        1 cagggacgag atatcatcga cctgagtttg ggtgagcctg actttgaaac tcctgagcat
       61 ataaaagctg cagctaaaaa ggctattgac gacggtttta cccattatac acctgttgcc
      121 ggctatttag atgtgcgcaa agccatagcc gaaaagttta tgcgagaaaa cggcctgctt
      181 tatacgccgg aacaaattgt catatctacc ggcgctaaac aaagcatcat caatgccgtg
      241 ttatgtctcg taaacccagg tgatgaagta attctgccta ctccattttg ggttagttat
      301 tctgcaatgg tgaagctggc agagggcaaa atagttgaaa tacctaccag tattgagtcc
      361 gatttcaaaa taacccctga acaactcgaa aaagccataa ccccgaaaac aaaactcatc
      421 atgttttcct ccccttgtaa ccctacaggg acagtttaca caaaagatga gctaaaggct
      481 ttggcagaag tggtagctcg ccacgaaaat atatatatca tcagtgatga gatatacgaa
      541 catatcaatt ttaccggcca gcacgccagt atggctcaat ttgactttat atacgataga
      601 gttataactg taaacggctt gtctaaagcc tatgccatga ccggctggcg tttaggatat
      661 attggtgcac cgctgtggat tgccaaagct tgtgataaaa ttcaggggca atatacatcg
      721 gcaacctgct caattacgca aagagcaaca attgcagcac ttgaaggtga tgttgaaact
      781 accctcacca tgaaagctgc ttttttacgc cgccgtgatc tcgtgtttca aatgatgcaa
      841 aatataaaag gacttaaatt caacaaccct cagggcgctt tttacttttt ccctgatgta
      901 agcaattatt ttggcaaaac ttataatggt aataccatca acaatgcaga tgacatggcc
      961 atgtatttat taaatgaagg taatttatcg gtggttaccg gtagtgcttt cggcgatcaa
     1021 aactgtattc gttttagtta tgctaccagc gatgataaat tggtagaagc gatgaaacga
     1081 ttaaaaattg ccttagaaaa attaatcaca caagaaatac cggtttcctg atatcttatg
     1141 gcaaatgcag ctgcaataaa aaaaatgttc tccgggtttc gccatattca tgtagcgata
     1201 attggcgata ttatggtaga caaatatgta tacggtaatg tagaccgcat ttcacctgaa
     1261 gcgccagttc ccgttttgga tgtaaaacaa aaagaaactc gtccgggcgg tgcagctaat
     1321 gttgccgtta atattaaagc actcgatgcc acaccttatt tattttctgt aattgggaac
     1381 gaaagtgaag gtgataattt aataggtcta ttaaaattaa aaggaattaa taccaaacat
     1441 atcattcgtt ccgaaaagcg aataactact gcaaaaacgc gtatcatttc aaaaaatcat
     1501 caaatgatgc gtattgatga agaagatatc agtgatttag atgaatcatt atcaaaaaag
     1561 ttgttgcagt ctatacttaa atttttgcaa aacacacctc cggatgtatg tatttttgag
     1621 gattacgata aaggtgtttt aacaccggaa atgatatcaa aaattataca tatatgtaac
     1681 caattaaata tacctgttat tgttgacccc aaaaagaaaa acttttttgc ctatcaacat
     1741 tgcacggtat tcaaaccaaa tattcgcgaa ataaatgaga gtttgcatac atcacttcaa
     1801 tatgtagatt taacttcatt aaataatatc accacattat tacatcagca acttacccat
     1861 aaaattacac tcatcacttt aggtgataaa ggcatttatt tacgtgccgg aaaaaaatca
     1921 tcgatacaaa aagcgcatgt acgtaatgtt gcagatgtta gtggtgcagg tgataccgta
     1981 gtaagtgtag ctgcattgtg tttggctgcg ggtgctgagc cttcccaaat ggccgctatt
     2041 gctaatatag caggtggttt agtgtgcgaa tcaccgggcg tggtatcaat cgataaatca
     2101 caattaatac aagagtgtat cgatttatta taaacaacat tttatttact tcttattgct
     2161 tcaacaggat tcattcgtga tgctgtaatt gcaggaataa ttccggcaat aacaccaagt
     2221 gcaaccgata tcgatgtacc tattaataca ttgctccatg ataaaataaa tccgaagcct
     2281 gttgctttat ccgcagcaaa cattaccagc caaaccagag ccaatccaaa taaacaaccg
     2341 attaatgcta atacaattgc ttcaattaaa aattcagtga gaatgagata attttttgca
     2401 cccaacgatt tttttatacc aatcacattg gtgcgttctt ttacgctcac aaacataata
     2461 ttggctatac caaacccacc aacaaggata ctaaatccac cgataataaa cccaacaata
     2521 ttcaacatgc cgaataaact atccaatgca tctgcagcaa gcgtaacctc attcacggca
     2581 aaatcactgg cttctgtcgg acgcaaattg cgtgcattcc gcataatggg ataaatttca
     2641 tcttttattt catctgctgt tataccatct ttcggccgaa cttctataac agggtctata
     2701 aaaccacttt tcatatccac taccctgcgg atataattaa atggaataat aatggaatta
     2761 tcgttgctga tatctataat actttcccct tccttttcta aaacaccaat taccttcatt
     2821 ttatactgtc ggcctgatac attaaaccag atatattttc caaccgggtc aatattgggg
     2881 ggaaataact gactggcaac ttcaaatccg ataatggcaa cttctgaacc cccaaccgat
     2941 tcgttaggaa caaagtagcg accaccttta aagttgagcg tatatatttc atcgtaattt
     3001 tctgtaacgc cgctaattgc tacattttgt aaatcgttat ccaggtattt tacattatct
     3061 gcagttagcc atacttctac agctaccgct tttgctttgg taagtttttc gttgaggagt
     3121 ttatattcct cataactcat ggtaggtcgt ttgatataat cccaccaggc atattcttca
     3181 ttattaaact gccagggcca acgctgaatg taaatcatat tggttcccag cttgtccaac
     3241 ccactttcca tgttccgctt aagcgagtca accgcgctta ataccgctat aatacagaat
     3301 atccctatcg ataccccgaa caacgacaag aaggttctga gcttgttatt tctcagttcc
     3361 tgcatcactt gcgggaaggt ttcagacaag gttcgaaaaa atacactcat tgtgcctcaa
     3421 agatatttta ttgtttccat tgaattagtt atagtttggt taattttgca cccgctctgt
     3481 tgattttctg cagatgcata attaaaaccc gtacgtttta tatgaaacta gatcggac
//
LOCUS       DAORKW010000395         3529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1433_length_3529_cov_5.271157, whole genome shotgun sequence.
ACCESSION   DAORKW010000395 DAORKW010000000
VERSION     DAORKW010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1433_length_3529_cov_5.271157"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLB46_13075"
     CDS             complement(<1..221)
                     /locus_tag="PLB46_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77502.1"
                     /translation="MTFYQPSRKKPILGLVIFILFIGVAAILFFSVRSCSNTGFFRSA
                     KSSDANYVYQVYPVITNDGVFFISLEGVF"
     gene            complement(239..1894)
                     /locus_tag="PLB46_13080"
     CDS             complement(239..1894)
                     /locus_tag="PLB46_13080"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRF77503.1"
                     /translation="MKIALAQINVHIGNFEANFNKIANFIEDAKNQQADIILFPEFSV
                     CGYPPRDFLDFDDFVDKCYEVIEKIKPLTQEIAVVVGSPSRNPKIEGKDLYNSAFFIF
                     EGEIKHIVNKALLPTYDIFDEYRYFEPATEFQNITFKGRKIALTVCEDLWNVGNDNPM
                     YKACPMDVLIKDKVQPEVMLNISASPFDYDHAAGRITTLKANTTKYKIPVFYVNHCGA
                     QTDLIFDGGSIVVNANSVVFDEMKYFEEQLSVYDLDEVIASKQTQTIDNVQPKVKIDL
                     IYRALVTGIRNYFEKLGFKKAILGLSGGVDSALTAVLAADALGAENVWGLLMPSQYST
                     GHSVTDALELANNLGIKHDTIAIKNMYDSVVGELTPLFNGKSFDVTEENIQARLRGLT
                     LMAMCNKHGYILLNTTNKSEAAVGYGTLYGDLCGGLAVLADVYKTEVYELVAYINREK
                     ETIPQHIITKAPSAELRPGQKDQDTLPPYDVLDKILFQYIELSQSPQDILNLGFDEHT
                     VRRTLKMVNSNEWKRFQFPPVLRVSPKAFGVGRRMPIEGKYLS"
     gene            complement(1953..2621)
                     /locus_tag="PLB46_13085"
     CDS             complement(1953..2621)
                     /locus_tag="PLB46_13085"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF77504.1"
                     /translation="MKKIIVTTMFFCALWSTVSAQEFRFGLTASPVFDWARVDGELYE
                     NVGTKLGFQYGLLFDQTIGSVERYAFSTGILINYVNVGISSTDTANIISAEWTARSQY
                     IEIPLTIRLRTNEVNYFSYYGQFGLTPGVCIKSRGDLSINDVAIYEDVNLRDKDNLTG
                     GQYQLFNVSLTLGIGAEYSIAENTNLIGGIFFQNGFANILDDDVDDNSAFLKQLGIRV
                     GVLF"
     gene            2657..>3529
                     /locus_tag="PLB46_13090"
     CDS             2657..>3529
                     /locus_tag="PLB46_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77505.1"
                     /translation="MVHKHRLFFVIFSISIAVVTMLTGCGKGNDNPHPDISKVPEQQV
                     EIVRLDQLIAAANAGNYNQVFSEIASQYPNIFEAYYSNFWGLSANDTSASYNLFDTLF
                     ENTAGNKWMMQLQDTISKIYPDLKEVEEELATAFRYYKYYFPDSSLPQLYTYTGPFVY
                     QTLFNETTLGIELDMYMGQHFGYYGAYESNMPLYITFRFDKPFISLNVMRSLMDGYLP
                     APVMEMSLLDAMVLEGKMMYYLDCVLPDMEDSVKMGYTEEQIEWCAGNEAEIWKFFAG
                     EELLFSKRTDDLRRY"
BASE COUNT         1104 a          645 c          622 g         1158 t
ORIGIN      
        1 ctgaatacac cttccagact gataaaaaaa acgccatcgt tcgtaatcac cggataaacc
       61 tggtatacat aatttgcgtc actgctttta gcagacctaa agaaaccagt attagagcaa
      121 ctgcgaaccg aaaaaaatag tattgctgct accccaataa ataatataaa aattactaac
      181 ccaaggatag gtttttttcg tgatggctgg taaaatgtca taggtattga gttaaatatc
      241 aggataaata tttgccttca attggcatac gtcgtccaac accaaaagct ttaggtgaaa
      301 cacgcaatac aggaggaaat tgaaaacgtt tccactcgtt gctatttacc attttgagtg
      361 ttctgcgaac agtatgttca tcgaaaccta aatttagtat atcctgtggg ctttgtgata
      421 attcgatgta ttgaaataaa attttatcca acacatcata tggtggcaag gtgtcctggt
      481 ctttttgacc cggtcttaat tctgcacttg gtgctttagt gataatgtgt tgtggtattg
      541 tttctttttc gcgattaatg taagcaacta actcgtatac ttctgttttg tacacatcgg
      601 ctaataccgc aagacctcca cataaatcgc catatagagt gccatatcca acagccgctt
      661 cgcttttatt tgttgtattt aataatatgt agccgtgttt attacacata gccattaatg
      721 ttaatccgcg taagcgtgct tgaatatttt cttcagtaac atcaaatgat tttccattaa
      781 acaaaggagt taattcgccc actactgaat cgtacatatt ttttatcgcg atagtatcgt
      841 gttttatccc taaattattc gctaattcaa gtgcatcagt tactgaatgt cctgtggaat
      901 attgtgaagg cattaataat ccccaaacgt tttcggcgcc taaagcatcg gcggctaaca
      961 cagcagtgag tgctgaatca acccctccgc ttaatcctaa aatagctttt ttaaatccga
     1021 gtttttcgaa ataatttctg atacctgtta ccagcgcccg ataaattaaa tcaattttta
     1081 ctttgggttg aacattatca atagtttgag tttgtttaga agcaattact tcatctaaat
     1141 cataaacaga taattgttct tcaaaatatt tcatctcatc aaataccact gaattggcat
     1201 ttaccactat actgccaccg tcaaaaatta aatcggtttg cgctccacag tgatttacat
     1261 aaaaaaccgg aatcttatat tttgttgtat ttgcttttaa cgttgtaatt cttcctgcag
     1321 catggtcata atcaaaaggt gatgcagaga tattcagcat gacttccggt tgtactttat
     1381 ctttaataag tacatccatc gggcaggctt tatacatggg attatcgtta cctacattcc
     1441 ataaatcttc acaaacagtg agggcaattt ttctgccttt aaaagtaata ttttgaaatt
     1501 cggttgccgg ctcaaaatag cgatattcat caaaaatatc ataagtaggt aataatgcct
     1561 tatttacaat atgttttatc tctccctcaa aaataaaaaa tgccgaatta tataaatctt
     1621 tgccttcaat ttttgggttg cgactaggtg aaccaactac aactgcaatt tcttgcgtga
     1681 gtggttttat tttttcaatt acttcgtaac atttgtctac aaaatcgtca aaatcaagaa
     1741 agtcgcgggg agggtagcca caaacactaa attctgggaa taaaataata tcagcctgtt
     1801 ggtttttagc atcttcaata aaattggcta ttttattaaa gttggcctcg aaattgccga
     1861 tatggacgtt aatctgagct aatgcaattt tcataataaa aaagtcacgc cttttgagcg
     1921 tgacaaattt agatgttatt tgttaaaccg tttcagaaca atacgccaac gcggatgccc
     1981 agttgtttaa gaaaagcact attgtcgtca acatcatcat caagaatatt ggcaaaccca
     2041 ttttggaaga aaataccacc tataagattg gtgttttcgg caattgagta ttctgcacca
     2101 attcccaagg ttaaactcac attaaataac tgatactgac ctccggttaa attgtcttta
     2161 tcgcgaaggt ttacatcttc gtaaatagca acatcattaa tagataaatc gccacgagat
     2221 tttatgcaaa cacccggcgt aagtccaaat tgtccatagt aactaaaata gtttacttca
     2281 ttggttctca acctgatagt aagtggaatt tcgatatact gtgatctcgc ggtccattca
     2341 gcactgataa tgttggctgt atctgttgag cttataccaa catttacata attaattaaa
     2401 attccagttg aaaatgcata ccgttcaacg ctaccaatag tttgatcgaa caataaacca
     2461 tattgaaagc ctagtttggt gcctacgttt tcatataatt cgccatcaac tcttgcccaa
     2521 tcgaaaaccg gagaggctgt taagccaaat cggaactcct gagcgcttac ggttgaccat
     2581 aaagcgcaaa aaaacatggt tgttactata attttcttca taccttatta gttttacatt
     2641 ttaaaattag atttacatgg tgcacaaaca taggttattt tttgtaattt ttagtatatc
     2701 aattgcggta gtaactatgt taacgggttg cggcaaaggt aacgataatc ctcatcctga
     2761 tatttctaaa gtgcctgaac agcaggtgga aattgttaga cttgatcaat tgatagctgc
     2821 tgcaaatgct ggcaattata atcaggtttt tagtgaaatt gcatctcaat atccaaacat
     2881 ttttgaagca tattattcca acttctgggg gctatcggca aatgacactt cagcaagtta
     2941 caaccttttt gacactttgt ttgaaaacac tgcaggaaat aagtggatga tgcaattgca
     3001 ggataccatt tcaaaaattt atcctgattt gaaagaagta gaagaagaat tggcaacagc
     3061 cttcaggtat tataaatatt attttccgga ctcctcacta ccacaacttt acacatatac
     3121 cggtccgttt gtttaccaaa ccctatttaa cgaaaccact ttgggtattg agttagatat
     3181 gtacatgggc caacatttcg gttattatgg agcttatgaa agtaatatgc cgttgtatat
     3241 cacttttcgg ttcgacaagc cttttatttc acttaatgtg atgaggagtt tgatggatgg
     3301 atatttacct gcacctgtta tggaaatgtc gttattggat gccatggttt tagagggtaa
     3361 aatgatgtat tatttagatt gtgtgttgcc cgatatggag gattctgtaa aaatgggtta
     3421 tactgaagaa caaattgaat ggtgtgctgg aaatgaagct gaaatttgga aattttttgc
     3481 cggcgaagag ttactgtttt caaaaagaac ggacgattta cgcaggtat
//
LOCUS       DAORKW010000396         3528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1434_length_3528_cov_4.532393, whole genome shotgun sequence.
ACCESSION   DAORKW010000396 DAORKW010000000
VERSION     DAORKW010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1434_length_3528_cov_4.532393"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..1050)
                     /locus_tag="PLB46_13095"
     CDS             complement(337..1050)
                     /locus_tag="PLB46_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRF77506.1"
                     /translation="MSQKINCLIVDDEPLAQQVIETYIQKMPSLVLVGKCDNAMEAYE
                     VLHNEKIDVMFLDIQMPVITGVEFLRTLQNPPAVIFTTAYPDFAMEGYDLNITDYLLK
                     PVSFERFLKAVNKATEHIALQQQSAQENDATATDYFFVKEDSKLVKINFSDIDHIECM
                     KDYAKIFTKQRMIVTHHTMKRFEEVLPPLQFVRIHRSYIVNIPAIQSIFGNIVETPKG
                     KLPIGANYKDELMKMVSGG"
     gene            complement(1047..2123)
                     /locus_tag="PLB46_13100"
     CDS             complement(1047..2123)
                     /locus_tag="PLB46_13100"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRF77507.1"
                     /translation="MSIGLTKVNKMFKSPSWRIFLHVAFWLFYLSLPFYAYLEASPSK
                     YAYLILILVNLAYLPFYYTLAYVLIPRFFNKRKWLYFVVYTLASYILFVWVCKGIEMA
                     FFDRLIYEKEIADFKEEIDSPLLDGFELLRMVFVTIIPLSIMLMRRFMRVYVERTELI
                     KTNTELELNFLKSQINPHFLFNTLNNIYSLSLQKSDKSPEMILKLSDLMRYMLYDCNV
                     QRVDLEREVQFLQDYIDLEKIRHGDKATINFTVTGVTHEKKVPPLLLIPFVENAFKHG
                     INAQFGNAWVRIDLQLDDKQHDFIFIIENNKPSSEEKLNHKNGGIGILNAQKRLHMLY
                     PDAHTLFIRENESTYIIELRIPKL"
     gene            2445..3251
                     /locus_tag="PLB46_13105"
     CDS             2445..3251
                     /locus_tag="PLB46_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRF77508.1"
                     /translation="MAQSAAKTTKKTTVNSLGILVIAINLVIGILFYVFVAGDKANFT
                     EDGHPKPGNYLALVHEGGFIVPVLLALFLTVCVFAVERVLTITKAKGNGSIQAFLQKV
                     KYHLTNKDIDAALKECDRQKGSVANVVNAGLHKYAEMKSDNALTTEQKVLNISKEIEE
                     ATALEMPALEKHLPILATIASIGTLIALLGTVLGMIRAFSALATEGTPDPSALSAGIS
                     EALINTAIGIGTSAFAIIFYNIFTGMIDKLTYGIDEVGFTITQTFSATEK"
     gene            3290..>3528
                     /locus_tag="PLB46_13110"
     CDS             3290..>3528
                     /locus_tag="PLB46_13110"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRF77509.1"
                     /translation="MPKIKVKRSSTIVDMTAMTDVSFLLLTFFILTAKFRPAQPVVID
                     MPSSESEIPVPDNMMVITVDKEGMIYFSVEHPKVQV"
BASE COUNT         1160 a          675 c          601 g         1092 t
ORIGIN      
        1 ggaggaaaag aataaaaaag caatggctca gaaggacttt gataacactc gctaccccca
       61 tgaaatcaca gtataaatat attcaatata aatttaccaa acaatatgtt taattggata
      121 tataaaaaag ccataaattg tctaattgta accctttatt atctattgaa attaaataaa
      181 atccagttga gaaatttttt atttccacta tttgaattgg attcgttatc attttactta
      241 tttacaattt tacctgtcga attaaaaatc aataaaaaac gatattgaag agccttattt
      301 attatttcat aaatgatgaa aacacttttt tagtactcac ccccccgaaa ccattttcat
      361 caattcatct ttataattgg caccaatggg taatttacct ttaggtgttt caactatatt
      421 gccgaaaata gattgaatgg caggaatgtt tacgatataa cttcggtgta tgcggacaaa
      481 ttgtagtgga ggcaaaactt cctcaaatcg tttcatggta tgatgtgtta cgatcatacg
      541 ttgtttggtg aaaattttcg cgtaatcttt catacattca atatggtcaa tatccgaaaa
      601 attgattttc accaacttac tgtcttcttt tacaaaaaaa taatcggttg ctgttgcatc
      661 attttcctgt gccgattgtt gctgcaatgc aatatgttcg gttgctttgt tcaccgcttt
      721 taaaaatcgt tcaaatgaaa cgggttttaa taaataatcg gtaatattta aatcataccc
      781 ttccatagca aaatcaggat aagcagtagt aaaaattacc gccggtggat tttgtaatgt
      841 tcgcaaaaat tcaacacctg tgattaccgg catctgaata tctaaaaaca tgacatcaat
      901 tttttcgttg tgtaatactt catacgcttc catcgcatta tcacatttgc ccaccaacac
      961 cagcgacggc attttttgaa tatatgtttc tattacctgt tgcgccaaag gttcatcatc
     1021 aacaatcagg cagtttattt tttgactcat aatttaggta ttcgtagttc aatgatataa
     1081 gttgattcat tttcacggat aaataatgta tgtgcatcgg gatataacat gtgtaatctt
     1141 ttttgcgcat tcaaaatacc tatcccaccg tttttgtgat tgagtttttc ttcgctcgaa
     1201 ggtttattat tttcaattat aaaaataaaa tcatgttgtt tgtcatccaa ctgtaaatca
     1261 atacgcaccc atgcattccc aaattgtgca ttaataccgt gtttaaatgc attttctaca
     1321 aacgggatta ataataaagg cggtaccttt ttttcgtgtg ttacaccggt aaccgtaaaa
     1381 ttgattgtag ccttatcgcc gtgacgaatt ttttcaaggt cgatataatc ctgtaaaaat
     1441 tgcacttctc tttccaggtc aacgcgctgc acattacaat catataacat ataccgcatt
     1501 aaatcgctca gctttaaaat catttccggc gacttatccg atttttgcag cgataaagaa
     1561 taaatattat ttaaggtatt aaataaaaaa tgcgggttaa tctgcgattt gagaaaattt
     1621 aattccaact ccgtattggt ttttattaat tctgttcgct ccacatacac ccgcataaat
     1681 cggcgcatga gcatgatact taatggaata atggtaacaa ataccattct gagcagctca
     1741 aaaccatcta acagcggact gtcaatttct tctttgaaat cggctatctc cttttcataa
     1801 attaaacggt cgaagaaggc catttcaatc cccttgcaca cccatacaaa caaaatataa
     1861 ctggcaaggg tatacaccac aaaatacagc catttgcgtt tgttaaaaaa tcggggaata
     1921 agcacataag ccagggtata ataaaagggc aaataagcca aattgaccaa aatgagtatg
     1981 agataggcat atttcgaagg actggcctcc aaataggcat aaaaaggcaa cgacaggtaa
     2041 aacagccaga aagccacatg taagaaaata cgccatgaag gcgatttgaa cattttattc
     2101 acttttgtta aacctatgct catagtgcaa agttgtaaag attatggcaa ggcagtttct
     2161 tttttcgaca aattgagccc actgttcgac aaaacaggcc gtttggcttg attttcaatg
     2221 ggttattgtc aaatttgtgt aaaacagccg ggctaatata ccaaagcaat taaggcatga
     2281 gtttatactt aaattgcggc aatacaacaa tttggcgact ttgcggttaa cataaaaata
     2341 ataaagtgaa attttaaaaa cgttaattgc aaaacgcttt ttttaaaaat taatctatat
     2401 ttgcccacta taacatttat taaaaggaaa ttaaaattaa ccttatggct caatcagcag
     2461 caaaaacaac aaaaaagacc actgtaaaca gtcttggaat cttggttatc gcaattaatc
     2521 ttgtgatagg tattttattt tatgtcttcg tagcaggtga caaagccaac tttacggaag
     2581 acggtcaccc aaaaccgggc aactaccttg cattagtgca cgaagggggg ttcatcgttc
     2641 ctgtattatt agcgttattc cttacggttt gcgtttttgc agttgaacgt gtactcacaa
     2701 tcaccaaagc aaaaggcaac ggttctatcc aggcgttttt acaaaaggtg aaataccatc
     2761 ttaccaacaa agacattgac gctgccttaa aagagtgcga ccgtcagaaa ggttctgtag
     2821 ctaatgttgt aaacgcaggt ttacataaat acgcagaaat gaaatccgac aatgcgttaa
     2881 ccactgagca aaaagtgtta aacatttcaa aagaaattga agaagcaact gcattagaaa
     2941 tgccggcttt agaaaagcac cttccaattt tggcaactat cgcctctatc ggaacactca
     3001 tcgccctcct cggaacggta ttgggtatga ttcgtgcgtt ctctgccctt gctactgaag
     3061 gtacacctga cccaagtgct ttgtctgcag gtatctcaga agcacttatt aatacagcta
     3121 tcggtatcgg aacttcagct ttcgccatca ttttctataa catttttaca ggaatgattg
     3181 ataagttgac ttacggaatt gacgaagttg gtttcaccat cacacaaacc ttctctgcaa
     3241 cagagaaata attcactaaa ttcagtgcac gtttcaaaac gaaataacga tgcctaaaat
     3301 aaaagtaaaa cgcagtagca ccatagtgga tatgaccgcc atgacggacg tgtcgttcct
     3361 gctgttgacg ttcttcatcc ttacggcaaa attccgcccg gctcagcctg tcgttatcga
     3421 tatgccaagc tccgagtcgg aaatacccgt tccggataat atgatggtta ttactgtaga
     3481 taaagaaggt atgatttact tttcggtaga acatccgaaa gtacaagt
//
LOCUS       DAORKW010000397         3525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1438_length_3525_cov_3.912680, whole genome shotgun sequence.
ACCESSION   DAORKW010000397 DAORKW010000000
VERSION     DAORKW010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1438_length_3525_cov_3.912680"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1675
                     /locus_tag="PLB46_13115"
     CDS             <1..1675
                     /locus_tag="PLB46_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF77510.1"
                     /translation="INGSDAGDTYLFRTGDLIIDHSKQYVGQLQHGDELMNKKLNLVY
                     GADGIYTAPDTENTINGKNEEDDNILEAGAYIQADYAVATKFNLIGAMRYDYHNFVNQ
                     PFFSPRAAAVYKANNKNTFRLTYNRSFSAPSSNNLNLDILQLGDLTGYAALFQGYLGS
                     DYVPNMPLYAIGNRDGFHYSYDDMGLPQFTSPYAEFLGYESDHYFSLDDGYEINNVIW
                     EATKGLLETAFNNTIPGLGPALVAWLPDSIDVYGNSVAALNLTTEEFDPIDEKNITDY
                     SAIKNSATNTIELGWKGSLFNDKVFMMVDVYYNMIQDYVSPLTDITPNVFIDGAELAA
                     YVTPIILENFYDTVGYNDNLADIVTATLDDNPAYGGNNDGTGVDEFINLVLAAGAGLP
                     VGTVSPMEYPNGAKYVTYVNLGDISVFGADLGMSWYVNDDLTMQFSYSWVDKDSVELP
                     GAQLGYVGLNAPKNKLSVRGFYDIPKIDLNVGLNFRWQSSFPANSGAYVGTVEEIHDL
                     DLTLNYTPDYIKDTQFTLLVTNLYNHEQQYFVGAPKMGSSYFFKITKSF"
     gene            1760..2467
                     /locus_tag="PLB46_13120"
     CDS             1760..2467
                     /locus_tag="PLB46_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77511.1"
                     /translation="MITWLQSVFGKKLPTKRILLYGLQRSGTNYLETLIQLNYPSCKF
                     VNGPVRNEIIHKHYRLYDKKLCIPEPQFYNNEHYQQFADFEKALPFEAPDVYLIMSKD
                     PYSWYTSYIGWSKKNNWPKPPYHYIEEYNLFYGKWMDFAAANKRIVFIQYAEALTNPE
                     RVLQKIANALDLPVREKIKTTKKVYASRRFTSDKKDAFLHKSYLQKITPEDFVIINTL
                     LDTGLLPKLGYHIESTQ"
     gene            complement(2468..3253)
                     /gene="mazG"
                     /locus_tag="PLB46_13125"
     CDS             complement(2468..3253)
                     /gene="mazG"
                     /locus_tag="PLB46_13125"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRF77512.1"
                     /translation="MNISVEAKLEAFKRLLNIMDDLRSLCPWDKKQTIHTLRNLTIEE
                     TYELAESITANDWQGIKEELGDVLLHMIFYSKIGEEQEQFSIADVLNAQCEKLIHRHP
                     HIYGDVKVKDEEEVKKNWEQLKLKEGKKSVLQGVPQGLPAMIKAFRIQEKAAQVKFEW
                     ENTQQVWNKVEEEMMELKEVFSTPDYDKLRLEDEFGDLMFSMINLSRFIHIDPENALE
                     RTNKKFIHRFQYIEAKALEQNKSLKEMTLEEMDALWNEAKLQG"
BASE COUNT         1099 a          660 c          594 g         1172 t
ORIGIN      
        1 tatcaatggt agtgatgccg gtgatactta tttattcaga accggagatt taattatcga
       61 ccattcaaaa caatatgttg gccaattaca acatggtgat gagttgatga ataaaaaact
      121 gaacttggtg tatggtgctg atggtattta cactgcaccg gatactgaaa ataccattaa
      181 tggtaaaaat gaagaagacg ataatatttt agaagcaggt gcttatatac aggctgatta
      241 tgctgtagct accaaattta atttaattgg tgccatgcgt tacgattacc acaatttcgt
      301 gaatcaacca ttcttttcac cacgtgctgc agctgtatat aaagcaaata ataaaaatac
      361 attccgctta acgtataacc gctcgttttc tgctccaagt tctaacaacc tgaatttgga
      421 tatattgcaa ctcggtgatt taacaggtta tgcagcatta ttccagggat atcttggttc
      481 tgactatgtg cctaatatgc cgctttatgc aattggtaac cgcgatggtt tccattattc
      541 ttatgatgat atgggattac cgcaatttac atcaccatat gccgaatttt tagggtatga
      601 atcagaccat tatttttctt tagacgatgg ttatgaaata aataacgtta tctgggaagc
      661 aacaaaaggt ttattagaaa ctgccttcaa taataccatt ccgggtttag gacctgcgct
      721 tgttgcatgg ttacctgact caattgatgt atatggcaat agtgttgccg cattaaattt
      781 aactaccgaa gaatttgatc cgattgacga aaaaaatatt accgattact ccgctatcaa
      841 aaactcggca actaatacta tagaattagg ttggaaaggt tctttattta acgataaagt
      901 atttatgatg gtagatgtat attataatat gattcaggat tatgtgagcc cgcttactga
      961 catcacacca aacgtattta tcgatggtgc tgagttagct gcatacgtta caccaattat
     1021 tctggagaat ttttatgata cagttggcta taacgacaac ttagccgata ttgtaacagc
     1081 aactttagat gataaccctg cttacggagg taataacgat ggaactggtg tagatgaatt
     1141 tatcaattta gtgcttgctg caggtgcagg tttaccggtt ggaacagttt caccaatgga
     1201 atatccaaac ggtgcaaaat atgttacgta tgttaatctt ggagatattt cagtatttgg
     1261 tgctgattta ggaatgagtt ggtatgtgaa cgatgacctg accatgcaat tttcttattc
     1321 ttgggtagat aaggattcag ttgaattacc gggagcacaa ttaggatatg ttggtttgaa
     1381 tgcaccaaaa aataaattgt cggttcgtgg attttatgat atcccaaaaa tagatttaaa
     1441 cgtagggttg aacttccgtt ggcaatcttc attccctgca aactctggtg catacgttgg
     1501 tactgttgaa gaaatccatg accttgatct taccttaaat tacacaccgg actatataaa
     1561 agatactcag tttactttat tggttaccaa tttatataat cacgaacagc agtattttgt
     1621 tggagcccct aaaatgggta gttcctactt ctttaaaatt actaagtcgt tttaagttac
     1681 atttgaaata cccgctatat ttaaagtccg cttcggcgga cttttttgtt gttgtaaatt
     1741 cccactatct tcgcagtata tgattacctg gttgcaatca gttttcggaa aaaaacttcc
     1801 aacaaaacgc attttattgt atggcttgca acgcagtggc actaactatc ttgaaacact
     1861 aattcaattg aattatccct cttgtaaatt tgtaaatggg ccggttagaa atgaaataat
     1921 acataaacat tacaggttgt acgataaaaa attatgtata cctgaaccac aattttacaa
     1981 caacgaacat tatcaacagt ttgccgattt cgaaaaagcg ttaccctttg aagcacctga
     2041 tgtttatctc attatgtcga aagaccctta tagttggtat accagttata taggttggag
     2101 taaaaaaaac aactggccaa aacctcctta ccattatata gaagaataca atttatttta
     2161 tggtaaatgg atggattttg ctgcagcaaa taaacgtata gtatttatcc aatatgcaga
     2221 agcgttaacc aaccctgaac gagtattaca aaaaattgcc aatgcacttg atcttccggt
     2281 tcgagaaaaa ataaaaacta caaaaaaggt atatgcttct cgcagattta cttccgataa
     2341 aaaggatgcc tttctccata aatcctatct tcaaaaaata acccctgaag attttgtaat
     2401 tataaacact ttactggata caggcttatt accaaaatta gggtatcata tagaatccac
     2461 tcaataatta cccctgcaac tttgcttcat tccaaagtgc atccatttct tccagggtca
     2521 tttcctttaa agatttgttt tgctctagag cctttgcctc aatgtattga aacctatgta
     2581 taaacttttt atttgtacgt tcaagtgcat tttccgggtc aatatgaata aacctcgata
     2641 gattaatcat cgaaaacatc aagtcaccaa actcatcttc caacctaagt ttatcataat
     2701 caggagttga aaatacttcc tttaactcca tcatttcttc ttccacttta ttccaaacct
     2761 gttgcgtatt ttcccattca aatttcacct gcgctgcttt ttcctgaatg cgaaacgctt
     2821 taatcattgc cggcaatccc tgtggaacac cttgcaggac tgatttttta ccctctttta
     2881 attttaattg ctcccaattc ttttttactt cctcttcatc tttcacttta acatcaccat
     2941 atatgtgtgg atggcggtga atcaattttt cacattgtgc atttaataca tcagctatcg
     3001 aaaactgttc ctgctcctca cctattttac tataaaaaat catgtgcagc aaaacatcac
     3061 ctaattcttc ttttataccc tgccagtcgt ttgccgttat cgactcggct aattcatacg
     3121 tttcctcaat agtcaaattt cgcaaggtat gaattgtttg ttttttgtcc caagggcata
     3181 aactacgcaa atcatccata atgttcagga ggcgtttaaa ggcttcaagt ttagcttcca
     3241 ctgaaatatt cataaccaaa tagttttaaa tgcaaaggtc tataattata ttggcatctt
     3301 aattgcgtgc atatggtatt gctcaataaa ttcaagatat tgatttttgt gattttacac
     3361 ctatatatat ataggtgtac tacttttggg caaaatgagg ttggtatgtt agtggacacg
     3421 attactaaaa acaatacgat acatccctta gctgagcaaa taaatgaccc ctttattaaa
     3481 acggtgcaac tcaatcgctc cgaccttgaa ttagcatccc ctgtt
//
LOCUS       DAORKW010000398         3511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1452_length_3511_cov_4.891782, whole genome shotgun sequence.
ACCESSION   DAORKW010000398 DAORKW010000000
VERSION     DAORKW010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1452_length_3511_cov_4.891782"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLB46_13130"
     CDS             complement(<1..231)
                     /locus_tag="PLB46_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77513.1"
                     /translation="MKIKMILLTAVVALSSSLASLVIYEKFLRDDDAQNGLQNPYQFS
                     SASFPVAPSGMAPVDFTYAAALTTPSVVHVKTT"
     gene            349..1125
                     /gene="dapF"
                     /locus_tag="PLB46_13135"
     CDS             349..1125
                     /gene="dapF"
                     /locus_tag="PLB46_13135"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRF77514.1"
                     /translation="MPLKFSKYQGTGNDFIIFDNRDGRIDHKNTQWFRHICDRRFGIG
                     ADGVMLLENEAGYDFKMVYYNADGNISSMCGNGGRCLVNYALRLGIVKDVYDFVAVDG
                     PHTAYVKNGIVHLKMNDVLSVSKGDNYYFLDTGSPHYVQFVEDVMQVDVKSTGATIRY
                     AEQFQPGGTNANFIDCLQNQLHIRTYERGVEDETLSCGTGVTASALAYAITKALQDGK
                     HTISLQTMGGELQVQFELKNGQFTDVWLIGPGELVFEGEI"
     gene            1170..1661
                     /locus_tag="PLB46_13140"
     CDS             1170..1661
                     /locus_tag="PLB46_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77515.1"
                     /translation="MQLYPLAVGNSWTYKMKDGNTYTNSITATEGQHYVMLNSASNIA
                     SKVYVDGNNYMTNAYTPDQFQAFLKEGAAVNDSWEIHFTANGIQSILVMTVREINSSI
                     EVEGKTYSQVLLLEADSKMNMNGTIMSLNYITKYYYAAGTGLVLTTSSYGDYHGLIDC
                     QLK"
     gene            1822..2778
                     /locus_tag="PLB46_13145"
     CDS             1822..2778
                     /locus_tag="PLB46_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRF77516.1"
                     /translation="MIKYYKKSNNTIVEIEKPEKDCWINVYPPFDHKRLATLSDAIDI
                     PIDYLLDSIDINERSRFEQDDNVKLIVINTPIENDMENSIDNDAFYITIPIGIILTQD
                     YNIIISSAQNKVIDWFFSIAIKHLSPSEKEMIVMKIFEKNVFYFIQYLNEMNKRRYLI
                     EKELMHSSRNTELAKLLNIQKSLVYFVTDLRANELLMMKIQRTNFLGIKTDEEKNDYL
                     SDIIIDSSQALEMATVYTNILNGTMDAFGSIISNNLNSVMKRLTSITIILMVPTLIAS
                     FFGMNFAHLPFEHANSGFFVIMFLSIFVSVILYLVFRKIRWF"
     gene            complement(2926..>3511)
                     /locus_tag="PLB46_13150"
     CDS             complement(2926..>3511)
                     /locus_tag="PLB46_13150"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77517.1"
                     /translation="VLVSPVCDLLPIELVEFTGWYNGVENELHWTTATEINSDYFEIQ
                     RSLNGIDFIAIGTTPAAGNSMELLHYIFYDDAPGSGINYYRLKMVDIDGSYDYSNIIA
                     IRLNNEVIQQITVFPNPANNEILIEYLAAEAENIQIDFLDATGRKVISENRILMQGVN
                     LLQYEIEHLPNATYFIQFTNTKTGNINGVQVLKY"
BASE COUNT         1120 a          656 c          632 g         1103 t
ORIGIN      
        1 agtagttttt acgtgaacaa cagatggtgt agtgagtgca gccgcatagg taaaatcaac
       61 cggtgccatc ccacttgggg caaccggaaa actggcgctc gaaaactgat atggattttg
      121 taatccattt tgcgcatcat catctcgtaa aaacttttca taaataacta gtgaagccag
      181 ggaactactc aaggctacca ccgctgttaa aagaatcatt tttattttca tatctaattg
      241 atttatgctt tttttgttaa caaatttcat gcactaaacg ccaactgaaa gattgtcgta
      301 ttttcgctta acaaatttta acccttttaa aggcttttaa cgtaaaacat gccattaaag
      361 ttttcaaaat accagggaac aggtaacgat tttatcattt ttgataatcg tgatggccgt
      421 attgaccata aaaatacaca gtggtttaga catatttgcg accgtcgatt tggaattggt
      481 gctgatggtg ttatgttgct cgaaaatgaa gcaggatacg acttcaaaat ggtgtattat
      541 aatgcagacg gcaatatcag ctccatgtgt ggcaacggcg gacgttgttt ggtgaattat
      601 gcattgcgcc ttggtattgt taaagatgta tatgattttg tggccgttga tggccctcac
      661 actgcgtatg taaaaaatgg cattgtccat ctcaaaatga atgacgtgct gtcagtttca
      721 aaaggcgaca attattattt cctggatacc ggttcaccgc attatgtgca atttgttgaa
      781 gacgtaatgc aggttgatgt aaaatcaacc ggagcgacca ttcgttatgc tgaacaattt
      841 caaccgggag gaacaaacgc gaattttatc gattgcctcc aaaatcaact gcatatacgc
      901 acctacgaac gcggtgttga agacgaaaca cttagctgcg gaacaggagt tacggcctct
      961 gccctcgcct acgccattac taaagccctt caggacggaa aacatacaat tagtcttcaa
     1021 actatgggtg gcgaattaca agttcaattt gagctgaaaa acggacagtt taccgacgta
     1081 tggctgattg gacccggaga actcgttttt gagggcgaga tataatatat ttgtctacct
     1141 attttcttat taaccctaat ttaaacccta tgcaacttta cccattagca gttggcaata
     1201 gctggactta caaaatgaaa gatggtaata cctataccaa ttcaattaca gcaacagaag
     1261 gtcaacatta tgtaatgctg aattcagcat ccaatattgc ttcaaaagtg tatgtagatg
     1321 gcaataatta catgaccaat gcgtataccc ctgaccagtt tcaagccttt ttgaaagaag
     1381 gtgcagctgt taatgatagc tgggaaattc actttaccgc aaatggcatt caaagtattt
     1441 tggtaatgac tgtccgagaa attaatagtt caatcgaagt agaaggcaaa acctattcgc
     1501 aggtgttatt gctggaagct gatagcaaaa tgaatatgaa cggcaccatc atgagtttaa
     1561 attacatcac caaatattat tatgcagccg gaaccggatt agtattaact acctcttcat
     1621 atggcgatta tcatgggctc atcgactgtc aactcaaata atttattgct ctaagcaggc
     1681 tttagccata ctcaaacatt gagcagcccg ggctaaaccc cttgttgctg taaggtttcc
     1741 aaaataaaat cctggtttgt ttgggaagat ataaacccgt atctttgcac gtccaaattt
     1801 ttaacctgca gatggaaagc gatgatcaaa tactacaaaa aatcgaataa caccattgta
     1861 gaaattgaaa agcctgaaaa agactgttgg ataaacgtat accccccctt cgaccataaa
     1921 cgacttgcta ctttaagtga tgctatcgat attccgatag attatttact tgactcaatt
     1981 gatatcaacg aacgctctcg ttttgagcag gatgacaacg taaaactcat cgtgattaat
     2041 acgcccatcg aaaacgatat ggagaattct attgacaacg atgcttttta tatcaccatc
     2101 cctattggca ttattctaac gcaggattat aacattatta tttcttctgc tcaaaataag
     2161 gttatcgact ggtttttttc tattgctatc aaacaccttt ctccaagcga gaaggaaatg
     2221 attgtgatga aaattttcga aaaaaacgtt ttctacttta ttcagtattt gaatgaaatg
     2281 aataaacgtc gttacctcat cgaaaaagag ctcatgcatt cgagtagaaa taccgaattg
     2341 gcaaaattgc taaatattca aaaatcactt gtttacttcg taaccgactt acgtgctaac
     2401 gaattactga tgatgaaaat tcagcgtacg aatttcctgg gaattaaaac tgatgaagag
     2461 aaaaatgatt atctaagcga tattattatt gatagcagtc aggctttgga aatggctact
     2521 gtatatacca atatccttaa cggcaccatg gatgcatttg gttcaattat ttcaaataat
     2581 ttgaactcgg tgatgaagcg acttacttcg attaccatca ttttaatggt accaaccctt
     2641 attgccagtt tctttggcat gaacttcgcc cacttgcctt ttgaacacgc caacagtggc
     2701 ttttttgtaa ttatgttttt atcgattttt gtttctgtaa tcctttattt ggtattcaga
     2761 aaaattaggt ggttctgata ttggctgctt gattacttcc cacataatat ttaattgtat
     2821 ggttatccta ttatttgtaa agcgcaatac aactatgcta taaccaaaac tcacatgtag
     2881 atttacatac aaaaagtaaa gttcaaatta atgattggaa aataatcagt acttcaacac
     2941 ctgaacacca tttatatttc ctgttttggt gttggtaaat tgtataaaat aagttgcgtt
     3001 gggtagatgt tcaatttcat attgcaacaa atttacaccc tgcattaata tcctattttc
     3061 actgattact tttcgtccgg tagcatctaa aaagtcgatt tgtatatttt cggcttcagc
     3121 tgcgagatat tcaattaaaa tttcgttatt ggcaggatta gggaatacag tgatttgttg
     3181 tatcacttcg ttattcaaac gaatggcaat aatattacta tagtcatacg aaccatcaat
     3241 atccaccatt tttaatctgt aataattaat tccggaaccc ggagcatcat cgtaaaatat
     3301 ataatgcaat aattccattg aatttccggc tgcaggtgtt gtgccaatgg caataaaatc
     3361 aattccattt aaggaacgtt gaatttcaaa ataatcggaa ttaatttctg ttgctgttgt
     3421 ccaatgtaat tcattttcaa ctccgttata ccagcctgta aattccacta attcaattgg
     3481 caataaatcg caaactggag aaaccaatac a
//
LOCUS       DAORKW010000399         3506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1457_length_3506_cov_4.831933, whole genome shotgun sequence.
ACCESSION   DAORKW010000399 DAORKW010000000
VERSION     DAORKW010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1457_length_3506_cov_4.831933"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLB46_13155"
     CDS             <1..231
                     /locus_tag="PLB46_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRF77518.1"
                     /translation="GIKQITIAGGVSANSYLRKRLIALGEKENWEVFIPKFEYCTDNA
                     AMIAITGYYKFLNKQFADQTTVPLARMSGLQS"
     gene            256..681
                     /locus_tag="PLB46_13160"
     CDS             256..681
                     /locus_tag="PLB46_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRF77519.1"
                     /translation="MSRIQVNFPEKHIFSTEIKVRIADLNYGGHLGNDSMLALLHEAR
                     VRYLKHFGYSEMDVEGTGIIMADVAIQFKTEAFYAERLTIAIAVGDFSRVSFDIYYKV
                     SSGNREVAIAKTGIVFFNYVKKKVAAVPEKFRLKMESKE"
     gene            925..>3506
                     /locus_tag="PLB46_13165"
     CDS             925..>3506
                     /locus_tag="PLB46_13165"
                     /inference="COORDINATES: protein motif:HMM:NF021665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HRF77520.1"
                     /translation="MMNFYGLKRIGAFLSFNLLVLSAFAQMDEDCNAHPDDELMHDEH
                     CAVFATVPIDDATHISITNGSWFNPATWNTGTVPNSGARVFIDSATTVTYDAVSETEI
                     NWVRIKGTLNFKNNVNTKLKVTTIVTDPIGHFNIGTTAEPILGTVTSKIIFPDQGIID
                     ISNDPYEFTKGIISHGFMVVNGEYKKTFCAISKGLNAGASNLKLAETPTGWKVGDQLV
                     LGGTNSNYAGDFVTNAKYHDEVLTITSISGKTIYFTNNATGGTTLQYEHKMPTGYGLK
                     IYVANLTRNVIFESENFATIPIDQRAHIMLMHNLAQSLSYAAFNGLGRTNKDILATDP
                     VVNDMGVQVSGGSNVRGRYAIHMHKAGTNNILAVPTLIKGNAIVDPTSWGIVNHQSNA
                     NIDDNVVFDFFGAAFVTEDGNELGTFNRNIAIKGRKATTHTNLDERTLNVDFGYEGNG
                     YWLQSSNISVENNIAVSCSGDAYKVFSDDASMPATHRFKIPKANILNPEIAGVDDSIY
                     TAVVPLRKFNGNIAYNCNSALMFWTHMLNIDNVGDFSSIENDPYTHSIMSVVQNCSFW
                     NLLAAGISVKYSGQIQFKNVLLLGDITSQFADSDWISGNPSGGFGFITSTVTGQIVYE
                     SLTVKGWKRAVVAGRADDLQSGDDFEYNYRTAKIIGGVFNNNTYNIYPEEGTDEYGAS
                     EYYKFPKYFEISGSPSFTSIIANANPVADYAYAANGGLSVKFNGVLSNDSDPGVITPG
                     EGNGIAAYVWNFGDGTTGYGVDPIHNFATAGNYNVVLTVYDSQGKTGSITKTITVANT
                     QYSNIFINTGFETGTLLSTSLAKSTREYVGNGWIHKSNWQLVNGKATIYLSDKWRRPL
                     VQIV"
BASE COUNT         1159 a          564 c          701 g         1082 t
ORIGIN      
        1 ggtataaaac aaattactat tgccggaggt gtatcagcca actcatattt acgtaaacga
       61 ttaattgcct taggtgaaaa agaaaactgg gaagtgttca tccctaaatt tgaatattgc
      121 accgacaatg cggcaatgat tgccattacc ggttattata aatttttgaa caaacaattt
      181 gccgaccaaa ccacggtgcc attggcgcgt atgtcaggat tacaatccta aagctacatt
      241 ttctattttt gtgggatgtc gagaattcag gtaaactttc cggaaaaaca tattttttct
      301 actgaaataa aagtgcgcat agccgattta aattatggcg ggcaccttgg taatgatagc
      361 atgttggcgc ttttgcacga agccagagtt agatatttaa aacatttcgg ttattcagaa
      421 atggatgtgg aaggaactgg cattattatg gctgacgttg ctatacaatt caaaacagaa
      481 gcattttatg ccgaaaggct aaccattgca atagcagtcg gcgattttag tcgggtatcg
      541 tttgatattt attataaagt atcaagtggt aaccgcgaag ttgctattgc taaaaccgga
      601 atagtatttt ttaattatgt gaaaaaaaag gttgctgctg ttcctgaaaa attccgttta
      661 aaaatggaaa gtaaggaatg atgcgaaatt ggcgaattta ttatttgaat tgattgtaag
      721 cacattagct agtaaatacc gcggttgtca acaattttag tcaatgcaca attaaaaata
      781 ggattaacta atgcacgaat attaaaataa tatttttact ttgcatcggt tattcgacat
      841 ccttcgtcta tacacacatc accctctttt accgcactcc cttgttgcca cacttttttg
      901 taactaaaca aacaattaag tgttatgatg aatttttatg gtctgaaaag aattggagcg
      961 tttttatctt ttaatttatt agtactttct gcattcgctc aaatggatga agattgtaat
     1021 gcacatcctg atgatgaatt aatgcatgat gaacattgcg ctgtttttgc cactgttcct
     1081 attgatgatg ccacacatat ttcaatcacc aacggttcgt ggtttaatcc tgctacctgg
     1141 aacactggca cagtgcctaa ttcaggtgcg agggtattta ttgattctgc aaccacagtt
     1201 acttatgatg cggtaagtga aaccgaaata aattgggtgc gcataaaagg cacgcttaat
     1261 tttaaaaaca atgtgaatac caaattaaag gttactacaa ttgtgaccga ccctattggt
     1321 cacttcaata ttggaaccac tgctgaacca attttaggaa cggttacttc gaaaattatt
     1381 tttcctgacc agggcattat cgatataagt aatgaccctt acgagtttac aaaaggaatt
     1441 ataagtcatg gttttatggt ggtgaacggg gaatacaaaa aaaccttttg tgcaatttca
     1501 aaaggattaa acgcaggggc atccaattta aaactggctg aaactccaac aggatggaaa
     1561 gttggtgacc agttagtatt aggtggaacc aatagtaatt atgcaggaga ttttgtaacc
     1621 aacgcaaaat atcacgatga agtacttact ataaccagta taagcggtaa aacaatttat
     1681 tttactaata atgcaacagg tggcacaaca cttcaatatg aacataaaat gccaaccggt
     1741 tacggattaa aaatatatgt agccaattta acgcgtaatg taatatttga atccgaaaat
     1801 tttgcaacaa ttccaattga ccagcgagca catattatgt tgatgcataa tctcgcgcaa
     1861 agtttatcat atgctgcgtt taatggatta ggcagaacca ataaagatat tttagcaacc
     1921 gaccctgtag taaatgatat gggtgtgcaa gtgagtggtg gatcaaatgt gagaggacga
     1981 tatgctattc atatgcataa agccggcacc aataatattt tggcggtgcc tacattaatt
     2041 aaaggcaatg caattgttga ccctaccagt tggggaattg taaatcatca aagtaatgct
     2101 aatattgatg ataatgtggt gttcgatttt tttggtgctg catttgtaac tgaagatggc
     2161 aacgaattag gaacatttaa tagaaacatt gcaattaaag gacgcaaagc tacaacacat
     2221 actaatttag atgaacgaac actcaatgta gattttggtt acgaaggaaa tggatactgg
     2281 ttgcaaagtt caaatatatc ggtagaaaat aatattgcgg tgagttgctc aggtgacgca
     2341 tacaaagtat ttagtgatga tgccagcatg cccgcaacac atcgatttaa aataccaaag
     2401 gcaaatattt taaatcccga aatcgcaggt gtcgacgact ctatttatac tgctgtagtt
     2461 ccacttcgaa aatttaatgg aaatattgcc tataactgta atagcgcttt aatgttttgg
     2521 acacatatgt taaatattga taatgtgggc gatttttcat ccattgaaaa tgacccctat
     2581 acacattcca ttatgagtgt agttcaaaat tgtagttttt ggaatttatt agcagcaggg
     2641 atttctgtaa aatattcagg gcaaatacaa tttaaaaatg ttttattgtt gggagatatc
     2701 acttcacagt ttgcagatag tgattggatt tcaggcaacc cgagtggggg atttggattt
     2761 attaccagca cggttaccgg acaaatcgtt tatgaatcat taacggtaaa aggttggaaa
     2821 cgtgcagttg ttgcaggtcg tgctgatgat ttacaaagtg gtgatgattt tgaatacaac
     2881 tacagaacag caaaaataat tggcggtgtt tttaataata atacctataa catatatccc
     2941 gaagaaggaa cagatgaata tggcgcttct gaatattata aatttccaaa atattttgaa
     3001 atttccggtt cgccttcatt tacatcaata attgctaatg ctaatccggt ggcagattat
     3061 gcttatgcag ccaatggtgg attaagtgtg aaatttaatg gtgtactttc taatgatagt
     3121 gacccgggtg ttattacgcc tggtgaagga aatggaattg ccgcttatgt ctggaatttt
     3181 ggtgatggca ctactggtta tggtgtggac ccaattcaca attttgcaac cgctggtaat
     3241 tataatgttg tgcttactgt ttatgacagt cagggaaaaa caggttcaat cacaaaaaca
     3301 attacagttg ctaatacgca atacagtaat atttttatca atacaggatt tgaaaccgga
     3361 acattgttat ctacatcatt ggcaaaaagt acacgtgaat atgttggtaa tggctggatt
     3421 cacaaaagca attggcaatt agtaaatggc aaagccacta tttatttaag cgataaatgg
     3481 cgacgaccac tggttcaaat tgtaaa
//
LOCUS       DAORKW010000400         3504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1458_length_3504_cov_4.902001, whole genome shotgun sequence.
ACCESSION   DAORKW010000400 DAORKW010000000
VERSION     DAORKW010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1458_length_3504_cov_4.902001"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1371)
                     /locus_tag="PLB46_13170"
     CDS             complement(55..1371)
                     /locus_tag="PLB46_13170"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HRF77521.1"
                     /translation="MLKKLLYFFGLVCLVHIAKANSDSVKTATTDFSYSAPTTIFGIY
                     DPLNVNLNGKKIAFSAYGDVYSASNCFSASFIQNFFGSGFITEEMKNDASAKLSDKNT
                     IGVDLSSGLWMMMALKNNPSMYLLAGVDYNFEESCQFTADLFHLVFYGNYDLQDYTAV
                     MSDSKFSLTNVMEYKIGLMKHYTEDYNTYKLGASIGLVQGLSGLDIKAPSATLYTAED
                     GRYLDLDYDFTIKTSGKSKPSLTNFAGAGFSADIFAQVYFKGPEITVNAMANDLGMIF
                     WNNDPTKISADSVLHFEGVETNNLFSATDEATAGNSDSLLQILGVVEKSDVFTQALPS
                     RINISASKMFGSDYYFTLGGQYMLNTPYKPLVFMQFAKVFTPLQMAIGVNAHGGGYGV
                     FNAGIDISKTFGNMIELRLGSNSLLGLVAADAFTGIGGYGTLTVKL"
     gene            complement(1393..3465)
                     /locus_tag="PLB46_13175"
     CDS             complement(1393..3465)
                     /locus_tag="PLB46_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77522.1"
                     /translation="MYLNRKGILLLLLAVSVFSACRRSLDTGWDTRYLSPILTGELTI
                     RDLVPDSVTVVNADESVTLVYQTELLNYDLTEEAIEIPDTSVEYFVSLDSLTLEDQSM
                     SVALSLGQIAMDLGFPIGTLIIAADGSETTLDPIYNLSTDPTPVDATSFFETATFNAG
                     FLDISIGNGLPVDLDSVVFRLTNSVGGEIIVEDTFFHVPALGVPVVITKDLTGMTVEG
                     NLMGEIIHFSTPGSPDVPVLIDTSDAVIINLIAYDMELQEAVAIFPAQNLINNANDVE
                     YNMGGPEFTEMGMEEGYVVIYVENTISDTIHIVYDIPGAVDEFGNSVHIETSVGPDEI
                     VDQAFDISGFTIDLKGSQGNAVNTFYQEFSARINYTGVPTYITLEDSLKVVYGLEGII
                     PNSLKGYLGQYEIAVADTTSGVELFDQFESGEIHFGDINIDLEITNGLGASGTVTINS
                     LGGTNTATGETVYLNCPTVVGVPININRALNNPYIPGYTTISLTSDNSNVNELVEIFP
                     DKLFYDVVMKVNPNGNEFNYQDFVIATSVIDIGFNISMPLDFFASDLTLLSEFPVTLD
                     AESGTEGIGNIDLTLFADNTFPLEANINIVFMDDLGTHLDSLNFSGFGIKAGVLAEDC
                     RVHEVTQTEIHQLVDGELKDAILNATKAVATIQFNTASIPSCSEIVKIYSDYKMAIQL
                     VGDVNYTF"
BASE COUNT         1127 a          692 c          609 g         1076 t
ORIGIN      
        1 aatagcgcta atttttcctt catcattaag ccttagtgga gtaaaatggg cgacttataa
       61 ttttaccgtt aaagtgccat aaccgccaat acctgtaaat gcatcagcag caactaagcc
      121 gagtaaacta ttactgccta atcgcaactc aatcatgttg ccaaatgttt tggagatgtc
      181 gatacctgca ttaaaaacac cataacctcc tccatgtgca tttacaccaa tggccatttg
      241 taatggcgta aacactttag caaattgcat aaatacgagt ggcttataag gtgtgttcaa
      301 catatattga ccacccaatg tgaaataata atcggagcca aacattttgc tggcactaat
      361 atttattctg gatggtaatg cttgtgtaaa tacatcagac ttttctacaa cacctaaaat
      421 ttgtaataat gaatcactat ttccggcagt ggcttcgtct gttgcggaaa ataaattatt
      481 ggtttctacg ccttcaaaat gtaaaacaga atctgcactt attttggttg ggtcgttatt
      541 ccaaaatatc atacccaaat cgttggccat ggcattaaca gtaatttcag gtcctttaaa
      601 ataaacctgc gcaaaaatat cggcgctaaa tcctgcacct gcaaaattgg ttaacgaagg
      661 ttttgattta cctgaagttt taatcgtaaa atcataatct aaatctaaat acctgccatc
      721 ttcagccgta tataatgttg ccgaaggtgc tttaatatct aaaccggata aaccttgaac
      781 taatcctata cttgcgccta atttgtaggt attataatct tcagtataat gtttcatcaa
      841 accaattttg tattccatta cattggttag actgaatttt gaatccgaca ttactgctgt
      901 ataatcttgc aaatcataat taccataaaa tactaaatga aataaatcgg cggtgaactg
      961 acagctttct tcgaaattat aatctacccc ggcaagtaaa tacatggaag gattattttt
     1021 taatgccatc atcatccata aacctgaaga aaggtctaca ccaattgtat ttttatcgga
     1081 taattttgct gaagcatcat ttttcatttc ttcagtaata aatccgctgc cgaaaaagtt
     1141 ttgaataaaa cttgcgctaa aacaattact tgcactatac acatcaccat aagcactaaa
     1201 cgcaattttt ttcccattta aatttacatt caatgggtcg taaatgccaa aaatagttgt
     1261 tggtgcactg tacgaaaaat cggtagtagc agttttaaca gaatcggaat ttgctttggc
     1321 tatgtgaacc aaacatacta atccgaaaaa atacaaaagt ttttttagca tcacgtgtca
     1381 gaaatcagcg gtttaaaagg tgtaatttac atctccaact aactggatcg ccattttata
     1441 atcactataa atttttacga tctcactgca gcttggaata gatgcggtat taaattggat
     1501 ggtagcaaca gcttttgttg cattgagtat agcgtctttt aattcgccgt ctactaattg
     1561 atgaatttca gtttgtgtta cttcatgcac ccggcaatct tctgcaagca caccggcttt
     1621 tatgccaaaa ccactaaaat tgagtgaatc gagatgtgtg ccgaggtcat ccataaatac
     1681 aatattaata ttggcctcaa gagggaaagt attatctgca aataaggtta aatcaatatt
     1741 gccaattcct tccgtaccac tttctgcatc aagtgttacc ggaaattcgc taagcaatgt
     1801 caagtcgctg gcaaaaaaat ctaatggcat actgatatta aatccaatat caattacgct
     1861 ggtggcaatt acaaaatcct ggtagttaaa ttcattaccg tttggattta ctttcattac
     1921 cacatcataa aataatttat ccggaaaaat ttccaccagt tcatttacat tggaattatc
     1981 tgaggtaagt gaaatggtag tatagccagg tatatacgga ttatttaacg ctctgttaat
     2041 attaatggga acaccaacaa cagttggaca atttaaataa actgtttctc ccgttgcggt
     2101 atttgttcca cctaaagaat taatggttac agttccgctg gcacctaatc cattggtaat
     2161 ttctaaatca atattaatat cgccgaaatg tatttctccg ctttcaaatt ggtcgaataa
     2221 ttcaacacct gaagtagtgt ccgcaacagc aatttcatat tggcctaaat atccttttaa
     2281 tgagttggga ataattcctt ctaacccata cactactttt aaactatctt caagtgtgat
     2341 ataagttggt acgccggtat aattaattct cgcgctaaat tcctgataaa atgtatttac
     2401 agcgtttccc tgactgcctt ttaaatcgat ggtaaatccg gaaatatcaa aagcctggtc
     2461 tacaatttca tcaggaccaa ccgatgtttc aatatgcacc gaattaccaa attcatcaac
     2521 cgctcccgga atatcataca caatatgaat ggtatcgctg atggtatttt caacataaat
     2581 aactacgtat ccttcttcca tacccatttc ggtaaactcg gggcctccca tattatattc
     2641 aacatcgttg gcattgttga tgaggttttg tgcagggaaa atggcaactg cttcttgcaa
     2701 ttccatatcg taagcaatca aattaataat cacggcatcg ctggtatcaa ttaaaacagg
     2761 aacatcggga ctgcccggtg tgctgaaatg aattatttct cccattaaat ttccttcaac
     2821 ggtcataccg gttaaatctt tggtaattac caccggaacg cctaatgcag gcacatggaa
     2881 aaaagtatct tctacaataa tttcaccacc aacagaattg gttaatctga atacaacaga
     2941 gtctaaatca accggcaatc cgttaccaat cgaaatgtct aaaaatcctg cattaaaagt
     3001 tgctgtttca aaaaatgagg tagcatcaac aggagttggg tcagttgata aattataaat
     3061 agggtcgaga gttgtttctg acccgtcagc tgcaataatt aatgtgccta tcggaaatcc
     3121 aaggtccatg gcaatttgac caagcgaaag cgcaaccgac atactttgat cctcaagtgt
     3181 gagggaatcg agtgaaacga aatattccac tgaagtatcg ggaatttcta tggcttcttc
     3241 ggtaaggtcg tagttgagca attcggtttg atacaccagg gttacacttt catctgcatt
     3301 caccacagta accgaatcag gtaccaggtc gcgaatggtt agttcgccgg ttaaaatagg
     3361 agaaaggtag cgggtatccc agccggtatc gaggcttctg cggcaagcgc tgaagacaga
     3421 tacagccaac aggaggagga gtatgccttt tctgtttaag tacattatgc tgtgaagata
     3481 aagaatattt taatgcagtt gcat
//
LOCUS       DAORKW010000401         3486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1470_length_3486_cov_4.687263, whole genome shotgun sequence.
ACCESSION   DAORKW010000401 DAORKW010000000
VERSION     DAORKW010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1470_length_3486_cov_4.687263"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PLB46_13180"
     CDS             <1..894
                     /locus_tag="PLB46_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HRF77523.1"
                     /translation="GLPIKSFPVAFLDFNNDGWQDLTIQTYDAENCDYDNAAEYFNKP
                     VKGEYSILYKNNGDGTFTDVTKEMNMQVAMTVMGLSFGDLDNDGWLDIYSGTGTPNYT
                     SLVPKRMFRNNEGKSYQDITHSGGFGHLQKGHGIGFGDVDNDGDQDVYEDMGGGYEGD
                     GFQGAFYENPGKSGNNWVSLQLEGVKANKSAMGSKVKITVENADKSTRDIYVEVTNGG
                     SFGVNSNRLEAGLGKAVAIKAIEISWLGPNTKQIITGVPMNSFVKIKEGEAAFEILPL
                     KSYKFAAMPMDTMMMHHGHQM"
     gene            1012..3402
                     /locus_tag="PLB46_13185"
     CDS             1012..3402
                     /locus_tag="PLB46_13185"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HRF77524.1"
                     /translation="MTTKTKRILIISGVAILVLAAGSYVMKSKIMALVKGNDNKRVAA
                     QQSADIKEIPWTATENTNQAMIDYLHKVYDAVNKPGLRFMNTAIAESLLALPVPEDAT
                     GKYQWYFDVCNQLLNCGRVNEAVAKIDEFEKSPQFKKLSDAQFGGWYYTKGLVYLRYG
                     EVENCIGLHNAESCIWPLSKAAQSMKKDGPTMAVKAYTDCLKYEPENLSAMWLMNIAY
                     QQLGTYPEAVPSQWLIKPESFKSEYDVPRFKNVAIDLGVDNPNMCGGVICDDFNNDDL
                     IDIFTCGWGLKERSFLLLNKGDGTFDDISKKAGLDEYPGGLMIQQTDYNNDGWLDVWV
                     SRGAWYSNFGILPSSLLRNNGDSTFTDVTREVGIWTTRPSQAATWADFNNDGWLDLYI
                     GNEASRKGDKINDCQLWINEKGKFREIAKEAGINVNSFVKGVTSGDYDNDGDADVYIS
                     ANGFKNYLFRNDTKKGSNKMKFTNVTKAAGVQGPESSFPCWFFDFDNDGWLDIMNFSY
                     SANISDNDIPAEYMGKPTQSDKTALYRNNGDGTFTDIAGKTGLANRTFLVMGSSYGDF
                     DMDGWIDFYVGTGKPDMRSLTPNRMLRNDGGKYYQEVSTAAGVGVLQKGHEISFADLN
                     NDGYPEIFAQMGGAYEASGFYDCLFENPASFQNNWISIDLTGTKSNKIARGARLKITT
                     VENGKERNIYEWVTQGSSFGANSLRQEIGLGKATAIKQIEITWPTSGIKTVLKDVAMN
                     QFIAVTEGTEGFTPITLTPFVFQSTPEKSFTHKMMMTMDHGDNARYMNMQHENMVH"
BASE COUNT         1142 a          544 c          737 g         1063 t
ORIGIN      
        1 ggtcttccaa ttaaaagttt ccctgttgca tttttagatt ttaataatga tggctggcag
       61 gatttaacta ttcaaactta tgatgcagaa aattgtgact acgacaatgc tgcagaatat
      121 tttaataaac ctgttaaagg ggaatattct attctttata aaaataatgg cgacggcaca
      181 tttactgatg ttactaaaga aatgaatatg caggttgcta tgacagttat gggtttaagt
      241 tttggcgatt tagataatga tggttggctc gatatttatt ctggcacagg cacaccaaac
      301 tatacgtctc ttgttcctaa aagaatgttc agaaataatg aaggtaaatc atatcaggat
      361 atcacccatt ccggtggatt cggacattta caaaaaggtc atggtattgg ttttggtgat
      421 gtagataacg atggtgacca ggatgtttat gaagatatgg gtggtggtta tgaaggcgac
      481 ggtttccagg gtgcttttta cgaaaacccg ggcaaatcag gcaataattg ggtgagttta
      541 cagttggaag gcgttaaagc taacaagtcc gcaatgggtt caaaagtgaa aattaccgta
      601 gaaaatgcag ataaatcgac ccgcgacatt tatgttgaag ttaccaatgg cggtagtttt
      661 ggcgtaaatt ctaatcgcct tgaagccggt ttaggtaaag cagttgccat caaagcgatt
      721 gaaattagct ggttggggcc aaacaccaaa caaattatca cgggtgttcc tatgaattca
      781 tttgtcaaaa taaaggaagg ggaagccgct ttcgaaatat tgcctttaaa gagttataaa
      841 ttcgctgcaa tgcctatgga tactatgatg atgcatcatg gacaccaaat gtaagcactg
      901 cgatttacaa aagttttgca gtgccgttaa aaaactttta actgtataaa cacataaaac
      961 acgaacaaac cgctcataaa agcggaaatt tgcactatca atcaaaatgt tatgactact
     1021 aaaaccaaac gtattttaat tatttcaggc gttgccattc ttgttttggc agcaggctcg
     1081 tatgttatga aaagtaaaat catggccctc gtaaaaggca atgataataa acgagttgct
     1141 gcacaacagt ctgccgatat caaagaaatt ccgtggacag ctacagaaaa caccaatcag
     1201 gcaatgattg attatctgca taaagtgtat gatgcagtaa ataaaccggg tttgcgtttt
     1261 atgaatactg ccattgctga aagtttattg gcattgcctg taccggagga tgctacagga
     1321 aaataccaat ggtattttga cgtgtgtaat caattattaa actgtggtcg cgttaacgaa
     1381 gctgttgcta aaattgatga gtttgaaaaa tcacctcagt ttaaaaaatt gagtgatgct
     1441 caatttggcg gatggtatta taccaaaggg ttagtgtatt tacgttatgg tgaagttgag
     1501 aattgtatcg gtttgcataa tgctgaatct tgcatttggc ctttatctaa agcagctcaa
     1561 agtatgaaaa aagatggccc tacaatggct gtaaaagcat atacggattg tttaaaatat
     1621 gagcctgaaa atttatctgc catgtggtta atgaatattg cttatcagca attaggtact
     1681 taccctgaag cagttccttc tcaatggtta ataaaaccgg aatcctttaa atctgaatac
     1741 gatgttccac gttttaaaaa tgttgcgatt gatttaggtg tagacaatcc aaatatgtgt
     1801 ggtggtgtta tttgcgatga ctttaataat gatgatttaa tcgacatatt cacttgcgga
     1861 tggggattaa aagaaagaag ttttctttta ttaaataaag gtgatggtac atttgacgat
     1921 atttcgaaaa aagccggttt agatgaatat cccggtggat taatgatcca gcaaactgat
     1981 tataacaatg atggttggtt agacgtttgg gtttcgcgtg gtgcatggta tagcaacttt
     2041 ggtattttac caagttcgtt attacgcaac aacggtgact ctacatttac agatgtaact
     2101 cgcgaagtag gtatctggac aacacgtcct tctcaagctg ccacatgggc tgattttaat
     2161 aacgatggct ggttagattt atatattggc aacgaagctt cacgtaaagg tgataaaatt
     2221 aacgactgcc aattatggat aaatgaaaaa ggtaaattcc gtgaaattgc taaagaggca
     2281 ggtattaatg ttaactcatt tgtaaaaggt gtaacctccg gtgattacga taatgatggt
     2341 gacgctgacg tatatatttc tgctaacgga tttaaaaatt atttattccg caacgatacg
     2401 aaaaaaggca gcaataaaat gaaatttaca aacgttacca aagcagcagg tgtgcaaggt
     2461 cctgaatcta gtttcccttg ttggttcttc gattttgata atgatggttg gttagatatt
     2521 atgaacttta gttattctgc aaatatttca gataatgata ttcctgctga atatatgggc
     2581 aaaccaacac aaagtgataa aactgcatta tatcgtaata atggtgatgg tacatttact
     2641 gatattgccg gcaaaaccgg attagctaat cgtaccttcc ttgtaatggg atcaagctat
     2701 ggcgattttg atatggatgg atggattgat ttttatgttg gaacaggtaa acctgatatg
     2761 cgcagtttaa caccaaatag gatgttgcgc aacgatggtg gtaaatacta ccaggaagtt
     2821 tcaactgctg caggtgttgg cgttttacaa aaaggtcacg aaatttcatt tgcagattta
     2881 aataacgatg gttatcctga aattttcgcc caaatgggtg gtgcttatga agcatcagga
     2941 ttttacgatt gtttattcga aaatcctgca tctttccaaa acaactggat ttcaatcgat
     3001 ttaacaggaa caaaatctaa taaaattgca cgcggtgcac gtttaaaaat tacgacagtt
     3061 gaaaacggta aagaacgtaa tatttacgaa tgggttaccc aaggttccag cttcggtgca
     3121 aatagtttac gtcaggaaat tggtttagga aaagctactg ctattaaaca aatcgaaatt
     3181 acctggccaa caagcggaat taaaactgta ttaaaagatg tagcaatgaa tcagtttatt
     3241 gctgttacag aaggcacaga aggatttact ccaattactt taactccatt tgtattccaa
     3301 agcacaccgg aaaaatcatt tacgcataaa atgatgatga cgatggatca tggtgacaac
     3361 gccagatata tgaatatgca acatgaaaat atggttcatt aattaaatcc aatactataa
     3421 atattttctg aggctgtctg caaaggcagc ctcttttttt gtgccggttg tgtagtgtaa
     3481 ttaaaa
//
LOCUS       DAORKW010000402         3486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1471_length_3486_cov_3.634509, whole genome shotgun sequence.
ACCESSION   DAORKW010000402 DAORKW010000000
VERSION     DAORKW010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1471_length_3486_cov_3.634509"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..900
                     /locus_tag="PLB46_13190"
     CDS             52..900
                     /locus_tag="PLB46_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF77525.1"
                     /translation="MSILFPTDFSEHSAKTFGFALDLAKKLNETITLVHVYPLPMNYP
                     SIDETRLGDVSEELINVTESAMEDRLRQFKTDLQDRYGSSHPELVRVNGILRMGFVGE
                     EVARAADEIKASYIVIGAKKPTGLKKFLGGSDVGAIIKKSHVPIFTVPENYHFKSIDK
                     IAYATDLTFNDNDVISRLMELALKFGSIIKCFHVHDSNLEVENAIISDFIERYKTEAN
                     NRTITFELVDNINITDGIDYYVNQHDIDLLVVLKQKTYWLDIFESSVTKKLVFHEDVP
                     MLIYHD"
     gene            complement(1058..2683)
                     /locus_tag="PLB46_13195"
     CDS             complement(1058..2683)
                     /locus_tag="PLB46_13195"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRF77526.1"
                     /translation="MIPFLFSLLWLSNPPEQIEADLIITNAKIYTVDSTFSIAQCMAI
                     DDGRIMEVGTTAAITEKYWSLNVTDMEGKVIYPGFIDGHCHFLQYGIEQSYTDLTGTT
                     SFLDVTERIKKHEISKTGGWVIGRGWDQNDWEIKEFPTKEALDKLFPSTPVYLVRVDG
                     HAALVNSKALELTGVTTTTHVDGGVVEVKQGQCTGILLDNALELVTKKMPTKSKTFLA
                     NAAMLAQEHCVAVGLTSVQDAGLDLWELQNLKMMDENGKLQMRIYAMANAGQKNYDYF
                     SANGMIYTDHLRIRAFKYYADGALGSRGAGLLQPYSDDPANYGIYYFSYDSLLAEAQK
                     IYNLGFQMCTHAIGDSANRMVLDIYGTVLQGKNDLRWRIEHCQVVNKNDFKKFSQFNI
                     LPAVQPTHATSDMYWADERLGESRIKTAYAYKQLLNQNGVLINGSDFPVEAINPLFGF
                     YAAVARKDQQFMPKDGFQKENALTRQEALKAMTIWAAYGAFEENEKGSLEAGKFADFV
                     VLEKDIMTIPEKELFSVKVLSTYIGGDVVYTTR"
     gene            complement(2680..3414)
                     /locus_tag="PLB46_13200"
     CDS             complement(2680..3414)
                     /locus_tag="PLB46_13200"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF77527.1"
                     /translation="MSATTELWYITGTSSGLGKAIAEKVLQRDGALVFGYARKNTIQH
                     PRYHHHFIDLSDVQAVQQIQFAPTTPDVKLIALINNAGLLGEMTSIGNIDNEKIVATY
                     HVNVVTPHILMNTFLQTYQHLEVKKCIINVSSGAAKTPYAGWSIYCATKSAIDMMSLV
                     AAKEQSLLPEKTATRIIALAPGVMDTNMQQQIRNADVADFPAKNKFVDLHKNQQLYSV
                     QDVAAKFIDVVLNDAPEQETIQRITL"
BASE COUNT         1055 a          678 c          606 g         1147 t
ORIGIN      
        1 atcactgttt tacagccttt tccgccgtaa atttacttca aaatacaggc tatgagcata
       61 ctctttccca ccgactttag tgaacattcc gcaaaaacct ttggatttgc gttggatttg
      121 gctaaaaaac tgaacgaaac catcactttg gtgcacgttt atccccttcc aatgaactac
      181 ccgagtattg atgaaacacg acttggagac gtttctgagg aactcatcaa tgtaacggaa
      241 agtgccatgg aggataggtt acgacagttt aagaccgatt tacaggacag gtatggcagc
      301 agtcaccccg aattggttcg tgtaaacggg attttacgaa tgggttttgt tggagaagaa
      361 gttgccagag ctgctgatga aataaaggcc agttacatcg taattggagc taaaaaacca
      421 accggtttga aaaagttttt ggggggaagt gatgttggag ccatcattaa aaaaagtcat
      481 gtgcccattt ttaccgttcc tgaaaactat catttcaagt cgattgataa aattgcctat
      541 gccacagatt taacatttaa cgataatgat gtgatttccc gtttgatgga attggcgttg
      601 aaattcggtt caattatcaa atgtttccac gtgcacgaca gtaatctgga agttgaaaat
      661 gctatcatca gcgattttat tgaacgttat aaaactgaag ccaacaatag aactattacg
      721 ttcgaactag tggataacat caatattacc gatggtattg attattatgt aaaccaacat
      781 gatattgatt tattggttgt gctgaaacaa aaaacatatt ggttagatat ttttgaaagc
      841 agtgttacca aaaaactggt gtttcacgaa gatgtaccga tgttgattta tcatgattga
      901 tatttattgg ctgatataaa attatattct ttgcgaaaga acagtcggaa ccacaaagcg
      961 cacgaaaaaa ttcacgcaaa ggccacaaaa gttggatgtg tagcagataa gtgatgcagc
     1021 taattaatag ttgtaaccat taactattta attattttca tctagttgta tacaccacat
     1081 ctccacctat ataagtggat aggactttta cgctaaacaa ttctttttcc ggtatggtca
     1141 tgatgtcttt ttctagtacc acaaaatcag cgaatttacc tgcttccaga ctgccttttt
     1201 cattttcttc aaatgctccg tatgccgccc aaatggtcat tgcttttaaa gcttcctgtc
     1261 gggttaatgc attttctttt tgaaatccat ctttaggcat aaattgttgg tcttttctgg
     1321 ctactgctgc gtaaaaacca aacaaaggat taattgcttc tacaggaaaa tcgctgccat
     1381 taatcagcac accattttga tttaataatt gtttataggc ataagccgtt tttattctac
     1441 tttctcccaa acgttcatct gcccaataca tatcgcttgt ggcatgtgta ggttgaactg
     1501 caggcaaaat attaaattga ctaaattttt tgaaatcatt tttattaact acctggcaat
     1561 gttctattct ccagcgcaaa tcattttttc cttgtaaaac ggttccataa atatcgagta
     1621 ccattctatt agcactatcg ccaatggcat gtgtacacat ttgaaatccg aggttataaa
     1681 ttttttgtgc ttctgctaat aatgagtcgt aactaaaata ataaatgcca taatttgcag
     1741 gatcatcact atatggttgt aataaaccgg cacctctgct acccaatgca ccatcggcat
     1801 aatatttaaa tgcgcgaata cgcaaatggt cagtatatat cattccattg gcgctgaaat
     1861 aatcgtagtt tttttgacct gcattggcca tggcataaat gcgcatttgc aatttgccat
     1921 tttcatccat catttttaaa ttttgtaatt cccacaaatc cagacctgca tcctgcacag
     1981 aagttaatcc aacagcaaca caatgttcct gagctaacat cgcagcattg gctaaaaatg
     2041 ttttactttt tgtcggcatt tttttggtta ctaattccaa tgcattatcc aataaaattc
     2101 cggtacattg tccttgtttt acttccacta ctccaccatc aacatgtgtg gttgtagtta
     2161 ctcctgttaa ttctaaggct tttgaattta ctaaggcagc atgtccatcc acccgcacca
     2221 aatacaccgg tgttgacgga aataatttat ctaaagcttc tttggttgga aattctttta
     2281 tttcccaatc attttggtcc catcctctgc caataaccca tccgccggtt ttgctgattt
     2341 cgtgtttttt aatacgttcc gtaacatcta aaaaagaagt tgtgcctgtt aaatctgtat
     2401 aagattgttc gataccgtat tgtaaaaaat gacaatgccc atcaataaat ccgggataaa
     2461 ttactttccc ttccatatcg gtcacattca gcgaccaata tttttcagta atagcagctg
     2521 ttgttccaac ttccataatt ctgccatcat ctatcgccat acattgagca atagaaaatg
     2581 tggaatcaac tgtataaatt ttagcgttgg taataattaa atccgcctca atttgttcgg
     2641 gtggatttga taaccataac agggaaaata aaaaaggtat cataatgtga tgcgttgaat
     2701 ggtttcctgt tcaggagcgt cattcaaaac cacatctata aacttagcgg caacgtcctg
     2761 cacactataa agttgctgat ttttgtgcaa atcgacaaat ttatttttag ccggaaaatc
     2821 cgctacatca gcatttcgta tttgctgttg catattagta tccatcacgc ccggagccag
     2881 cgcgataatt cgtgttgccg ttttttcagg caataatgat tgttcttttg cggcaactaa
     2941 actcatcata tcaattgctg attttgttgc acaataaata ctccatcctg catacggagt
     3001 ttttgcagca ccggagctga cattaataat acattttttt acctcgagat gctgatacgt
     3061 ttgcaaaaat gtattcatta aaatatgtgg tgtaacaaca ttcacatgat aagtagctac
     3121 aattttttca ttgtcgatat tacctataga tgtcatttca cctaataatc ctgcattatt
     3181 aatcagtgct attaatttga catcaggtgt tgtgggcgca aattgaattt gttgcacagc
     3241 ctgcacatcg gataaatcga taaagtgatg atgataacgc ggatgctgaa tagtattttt
     3301 tcttgcatat ccaaatacca aagcgccatc tctttgtaag actttttccg caattgcttt
     3361 tcccaatccg gaagaggtgc ctgtgatata ccaaagttct gtagtagcgg acatcattaa
     3421 aaatttacta ttaggacatt tgtcctaaat taaacagcat atgatttgtg taacaaaaga
     3481 taatca
//
LOCUS       DAORKW010000403         3482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1473_length_3482_cov_3.357747, whole genome shotgun sequence.
ACCESSION   DAORKW010000403 DAORKW010000000
VERSION     DAORKW010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1473_length_3482_cov_3.357747"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    28..127
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(505..2364)
                     /gene="hscA"
                     /locus_tag="PLB46_13205"
     CDS             complement(505..2364)
                     /gene="hscA"
                     /locus_tag="PLB46_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HRF77528.1"
                     /translation="MAQISINIKEGTISKPDIIVGIDLGTTNSLVAIINKAGMPEALC
                     DADKTAIVPSIVHFGKDGAITVGNSARQHLIDDTANTIYSVKRLMGKSYNDIAAFQQH
                     LGYKIIDDNSESLVKIKVGEKFYSPIELSAFILRELKLRAENVLQQQISKAVITVPAY
                     FNDAQRQATRDAGKLAGLDVLRIINEPTAASLAYGLGNVKNAQTIAVYDLGGGTFDIS
                     ILQLTPSENDGGYFEVLATNGNTFLGGDDFDRLIVEHWLQTNNIAVDSVDKQLMQTLR
                     LKAETAKKHLSANDNFEDTINDIVCRLSVNEFETLIKPLVNETIVCCKNALRDAGLKI
                     GDIDTIVMVGGSTRTPFVKKSITEFFNKPVNDSLNPDEVVALGAAIQADILAGNQKDM
                     LLLDITPLSLGIETMGGLMDVIIPRNTKIPTGAGRQYTTSVDGQSKLLIAVYQGERDL
                     VSHNRKLAEFTLSNIPGMPAGLPKIEIVFQLNADGILQVKAKELRSGVEQSIEVKPQY
                     GLTEEEMGGMLLESIKNAKSDMEQRAIIEARVEAQNMLDTCERFIVKNATHLSATEIS
                     GTRELMVALQNTLNSSDKNVIQQHIEALNEFTRPFAERLMDMAISMAMKGKTL"
     gene            2561..>3482
                     /locus_tag="PLB46_13210"
     CDS             2561..>3482
                     /locus_tag="PLB46_13210"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRF77529.1"
                     /translation="MYKSLLKPLFFKFQPEKAHHLALSLFSIALKIPGYGYFHDKKYL
                     KQYPNLERKVFGLTFKNPIGLAAGFDKNAAYIPLMARLGFGHIEVGTVTPLAQPGNDK
                     PRLFRLPKDEALINRMGFNNDGVDVMLQNLQKPAVIAARKKYQVIIGGNIGKNKVTAN
                     EDAWKDYVICFEKLFDWVDYFVINVSSPNTPGLRELQEKAPLTHIINKLQEINRTKSA
                     PKPLLLKIAPDLTTSQLLDIVSIAKETGLSGIIATNTTLDRSMLTTEAEKVAAIGAGG
                     LSGKPVAKISNEVLWYLRRELGNDMPIIGVG"
BASE COUNT         1058 a          732 c          577 g         1015 t
ORIGIN      
        1 cgtcagggcg acacaccgat aagatccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      121 nnnnnnnaaa ccaccaagtt gcattacaat accactagcc cgacagggcg acataccggt
      181 aagatccaaa ccaccaagtt gcattacaat accactagcc cgacagggcg acataccggt
      241 aacatccaaa ccaccaagat gaataacatg acacccagcc cgtcagggcg acataccggt
      301 aagatccaaa ccaccaagtt gcataacaat accaccagcc cgtcagggcg acacaccgat
      361 aagatccaaa ccaccaagtt gcattacaat accaccagcc cgtcagggtg atatactggt
      421 ttgacctaca gctctttaat tagacaaatc agaaaataag ttggagaaat tattagaacg
      481 ttacttatca atcaaacaaa cacattacaa cgttttacct ttcatggcca tactaattgc
      541 catatccatt aaacgctcag caaaaggcct ggtaaattca tttaatgctt caatatgttg
      601 ttgaataaca tttttatcgc ttgaatttaa agtattttgt aaagcgacca ttaattcacg
      661 tgttcctgaa atttcagttg cacttaagtg cgtggcattt ttcacaataa accgctcaca
      721 ggtatctaac atattttgag cctcaacacg cgcttcaata attgcccttt gttccatatc
      781 ggacttcgca tttttaatcg actccaataa catgccaccc atttcttctt ccgttaatcc
      841 gtattgcggt ttaacttcaa tagattgctc cacgccactg cgtaattctt tcgctttaac
      901 ctgcaaaata ccatccgcat ttaattgaaa aacaatttca attttaggta aacctgcggg
      961 cattccggga atattcgata atgtaaattc agcgagtttt ctattatgac taactaaatc
     1021 gcgctcaccc tgataaactg caatcaataa tttactctgc ccgtcaacac ttgttgtata
     1081 ttgtcttcct gcacctgttg gaattttggt attacgtgga ataatcacat ccattaaccc
     1141 acccatagtt tcaataccca aactcaatgg tgtgatgtca agcaataaca tatccttttg
     1201 attacctgct aaaatatctg cctgaattgc ggcgcctaaa gcaaccactt catccggatt
     1261 taaactatcg ttaaccggtt tattaaaaaa ttcggtaatc gattttttta caaatggtgt
     1321 tctggtactt ccgccaacca tcactatagt atctatatca ccgattttta atcccgcatc
     1381 acgcaatgca tttttgcaac aaactattgt ttcgttgaca agtggtttaa tcagtgtttc
     1441 aaattcgttt accgataagc ggcaaacaat atcattaatt gtatcttcaa aattatcgtt
     1501 agctgataaa tgttttttgg cagtttccgc ttttaatcgc agtgtttgca ttaattgttt
     1561 atctaccgaa tcaaccgcaa tattattagt ttgtaaccaa tgttcaacaa ttaagcgatc
     1621 gaaatcatct ccccctaaaa aagtattgcc attagtagct aaaacttcaa aatatcctcc
     1681 atcattttca gatggtgtga gttgtaaaat agaaatatca aaagtgcctc cacccaaatc
     1741 gtaaacagca attgtttgtg cattttttac attgcccagt ccgtatgcca aactcgctgc
     1801 agtaggttca tttataatac gcaacacgtc aagtccggca agtttacctg catcgcgtgt
     1861 agcttgtcgt tgagcatcat taaaatacgc gggaacggtt ataacagctt ttgaaatttg
     1921 ttgttgcaac acattttctg cacgcaattt taattcacgt aaaataaatg ctgataattc
     1981 tattggtgaa taaaactttt cgccaacttt aattttcacc agactttcac tgttatcatc
     2041 aataatttta tagcctaaat gttgctgaaa cgctgcaata tcgttgtatg atttacccat
     2101 taatcttttc acggaataaa tagtattggc agtatcatca atcaaatgct gacgtgcact
     2161 attgcctaca gtaattgcac catcttttcc gaaatgtaca atggatggaa ctatagcagt
     2221 tttatcggca tcacacaaag cctcaggcat acctgcttta tttataattg ccactaaact
     2281 attggttgta ccaaggtcta taccgacaat aatatcaggt ttactgattg ttccttcttt
     2341 tatatttata gatatttgag ccatgctgtt gtttaaaaca aaaattgaaa tgcaaagttg
     2401 ggtaaaaatt agggttttat gtaacctaat aattgctaat tcatgtaaaa gtttgacatt
     2461 attcttcagg aacgcataac aacacctttt aattcagaaa caacttcaat tcactcgaat
     2521 ttttactttt cttcaccgca acagcctaac tttgcgaaca atgtataagt cactgctcaa
     2581 acccttgttt tttaaatttc agccggaaaa agcacatcat ctggcacttt ctttattcag
     2641 tattgcatta aaaattcccg gctacggtta tttccacgat aaaaaatacc ttaagcagta
     2701 tcctaatctg gagcgtaaag tttttggatt gacgtttaaa aaccccatcg gattagcagc
     2761 aggctttgat aaaaatgcag cttatatacc gctgatggcg cgattaggat ttggtcatat
     2821 agaagtagga acagtaacgc ccttagcgca acctggtaac gacaaaccga gattatttcg
     2881 tttgccaaaa gacgaggctc tgattaacag aatgggtttt aacaacgatg gggtagatgt
     2941 tatgttgcaa aatctgcaaa aacccgccgt aattgctgca cgaaaaaaat atcaggtgat
     3001 aattggcgga aacatcggta aaaacaaggt tacagctaat gaagacgcct ggaaagacta
     3061 tgtgatttgt tttgaaaaac tattcgattg ggtagattat tttgtgataa atgtatcatc
     3121 acccaacaca ccggggttga gggaattgca ggaaaaagca ccgctgacgc atatcatcaa
     3181 caagctccag gaaataaatc gcacaaaatc tgcgccaaaa cccttgcttt taaagattgc
     3241 acccgaccta actacgtcgc aactacttga tattgtgtcg attgcgaagg aaaccggcct
     3301 cagtggtatt attgctacca acactacgct tgatcgctct atgcttacta cagaagccga
     3361 aaaggttgca gctattggtg caggcggttt gagtggaaaa cctgtcgcca aaatctccaa
     3421 tgaagtgctg tggtacctgc gtcgtgagtt gggtaacgac atgccgataa ttggtgttgg
     3481 tg
//
LOCUS       DAORKW010000404         3480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1474_length_3480_cov_5.081752, whole genome shotgun sequence.
ACCESSION   DAORKW010000404 DAORKW010000000
VERSION     DAORKW010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1474_length_3480_cov_5.081752"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLB46_13215"
     CDS             <1..383
                     /locus_tag="PLB46_13215"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRF77530.1"
                     /translation="LLLQTRGAAIINNYLLQNELYRIGGTTLLRGFDEQSIVVSQYHI
                     GTFELRYLLSQNAYAAIFADGGYAENAASTTLTADWPYGFGAGINFETKAGIFGLTYA
                     LGSREGNPVQFKNTKIHFGYVNYF"
     gene            384..1298
                     /locus_tag="PLB46_13220"
     CDS             384..1298
                     /locus_tag="PLB46_13220"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HRF77531.1"
                     /translation="MKQQLLLILLLVAGISFAFAQSDTGAAVQQNQAVPFSPQQPPPK
                     PAKVYPVDTQAVLEMLKFDKLGYDSLFRIKSGPVMIQKTEVKHVTARDFILPVLLLVM
                     LAYVTWLRYVFAKELGENLTVILNSNLGQQIYRDREFSANIFKLLTFVNFAFCAGVFV
                     YLLTQYFELELPFKLAIYNIGLCIGGLSVLYLLKGLVYRIIGAAFKLSAPLQFFRFNA
                     LVIYHLLGIGLLPFVILAAFAEPPVNNWAIFGALILIAIALLIRLVKGFASLRLTGRF
                     HFVYFLLYICALEIAPLLIAIKVFTNWA"
     gene            1320..2102
                     /locus_tag="PLB46_13225"
     CDS             1320..2102
                     /locus_tag="PLB46_13225"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRF77532.1"
                     /translation="MNNSQQPAIKPVKSILISQPKPPEIPKSPYQDLAEKYKIKIDFR
                     QFIQVRGLAAKDFRKQRINILDYTAVIFTSRNAIDHFFRICEELRIRMPEMTKYFCVT
                     EAIAVYLQKYIQYRKRKVFYGAGKETELFDLLKKHLSEKFIFPCTVNHKDVYTEYLKN
                     NNADFVEAMMYETVAADLTDMKGKIGYDMIVLFTPTGVASLFQNFPDFEQGDLRIGCM
                     GSATLKAMEEQHLRIDVKAPTPESPSIIMAIDNYLAKANKKK"
     gene            2181..>3480
                     /locus_tag="PLB46_13230"
     CDS             2181..>3480
                     /locus_tag="PLB46_13230"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRF77533.1"
                     /translation="MQQQFSNHLIHETSPYLLQHAHNPVEWFPWGELAFEKARKEDKP
                     ILISIGYAACHWCHVMEHESFENVATAEFMNTYFVNIKVDREERPDLDHIYMNTCQLL
                     TGAGGWPLNVFLTPELLPFSAGTYFPPKPGYGKPSWLDVLMYMKNVFTNERDKVEQQA
                     QSIREHLFKMDQSFIEKINLPEGTALFTKEELITTVTQLKKQFDTEDGGFGSAPKFPG
                     SMALLFLLRYNFFLKDEAVQQHIHLSLNKMMFGGIYDHIGGGFARYTVDKKWMIPHFE
                     KMLYDNALLISLYAEAYREQPNANYKSVIENTLAFVEREWMTSEGGFYASYDADSEGV
                     EGKFYTFTYNEIQTILKENFPLAEKVYQIKKDGNWEHTNILFRNNTNEALAAEMDISV
                     AELTQQLSAINKQLFDYRENRIKPGLDNKIILSWNAMMCSA"
BASE COUNT         1075 a          637 c          673 g         1095 t
ORIGIN      
        1 cattactact gcaaacaaga ggagcagcta tcatcaataa ttatttattg caaaatgaat
       61 tatatcggat aggaggcaca acattgttac gcggtttcga cgaacaaagt atcgttgttt
      121 ctcaatatca tatcggaaca ttcgaattgc gttatttatt gtcacaaaat gcatatgccg
      181 caatttttgc cgatggggga tatgctgaaa atgcagcaag caccacttta accgccgatt
      241 ggccttatgg ttttggtgca ggtataaatt ttgaaacaaa agcaggtatc tttggtctaa
      301 catatgcatt aggcagccgt gagggcaatc cggtgcagtt taaaaacacc aaaatacatt
      361 ttggttacgt gaattatttt taggtgaaac aacaactatt gttaatatta ttattggttg
      421 ccggtatttc atttgcgttc gcgcaatctg atacgggcgc tgctgtgcaa caaaaccagg
      481 ctgtaccatt ttcaccacag cagcctccac ctaagcctgc taaggtttac ccggttgata
      541 cacaggccgt tctggaaatg ctgaagttcg ataaattagg gtacgacagt ttgttcagga
      601 taaagagtgg gccggtgatg atacaaaaaa ccgaggtgaa acatgttact gcccgtgatt
      661 ttattttacc cgttttgtta cttgtcatgc tggcttatgt aacctggctg cgatatgtat
      721 ttgccaaaga attaggggaa aacctcactg taattctcaa tagcaattta ggccagcaga
      781 tttatcgcga cagagagttc tctgccaata ttttcaaatt gttaactttt gtcaactttg
      841 ccttttgtgc cggcgtattt gtgtatttgt taacacaata tttcgaactt gaactaccgt
      901 ttaaattagc tatttataat atcggcctct gtattggtgg attgtctgtt ttgtatttat
      961 tgaaagggtt ggtttaccgc ataattggag cagcattcaa gctttcagcc ccgcttcagt
     1021 tttttagatt taatgccttg gtaatttacc atttgttggg aattggcctg ttacccttcg
     1081 ttattttggc cgcatttgct gaaccacctg ttaataattg ggctattttt ggcgcactta
     1141 tccttattgc aattgcctta ttgataaggc ttgttaaggg ttttgcctca ctccgactta
     1201 cagggcggtt tcatttcgtg tattttttgc tgtatatttg cgccctcgaa atagcacctt
     1261 tgttgatagc cattaaggtt ttcaccaatt gggcttaggt gaagcaaaaa aaattgccga
     1321 tgaataacag ccaacaacct gcaattaagc cagtaaagtc aattttaatt tcacagccca
     1381 agccccctga aattcctaaa tcaccatatc aggatttggc tgagaaatat aaaatcaaaa
     1441 tcgactttcg tcagtttatt caggtaaggg gactagcggc taaagacttc agaaaacagc
     1501 ggatcaatat tctcgattac actgcagtta tttttaccag ccgaaatgct atcgaccatt
     1561 ttttcagaat ttgtgaagaa ctccgcattc gtatgccgga aatgaccaaa tatttttgtg
     1621 tcaccgaagc tattgcggtt tatctccaaa aatatattca ataccgcaaa cgcaaagtat
     1681 tttacggcgc tggtaaagaa actgaattat ttgatttgct taaaaagcac ctttcagaaa
     1741 aatttatttt tccatgtact gtcaatcata aagacgtata caccgaatac ctcaaaaata
     1801 ataatgccga ttttgttgaa gctatgatgt atgaaactgt agcggcagac cttaccgaca
     1861 tgaaaggaaa aatcggatac gacatgattg tattgtttac accaacaggt gttgcaagtt
     1921 tattccaaaa tttccctgat tttgaacaag gcgatcttcg cattggttgt atgggcagcg
     1981 ctacattaaa agctatggaa gaacaacatt tgcgtattga tgttaaagca cctacacccg
     2041 aatctccatc aataattatg gctatagata attatcttgc caaagccaat aagaaaaagt
     2101 aatcggttta tattttactt ttattttatc ttgctgaact tacggcaacc agatacgttt
     2161 gcacgtttaa ttaaactact atgcagcaac aattttccaa tcatcttata cacgaaactt
     2221 caccctattt attgcagcat gcgcataatc cggtggaatg gttcccttgg ggagaattag
     2281 ctttcgaaaa agctcgtaaa gaagataaac ctattttaat ttcgatagga tatgccgctt
     2341 gccactggtg tcatgtgatg gaacatgaaa gttttgaaaa tgttgctaca gcagagttca
     2401 tgaatacgta ctttgttaat attaaagtgg atagagaaga acgtcccgac ctcgatcata
     2461 tttatatgaa tacctgccaa ctattaacag gtgccggagg atggccatta aatgtttttc
     2521 ttactcctga attattgcct tttagtgccg gaacttattt ccctccaaaa ccgggttatg
     2581 gcaaaccttc gtggttggat gtgttgatgt acatgaaaaa tgttttcacc aacgagcgcg
     2641 ataaagttga acaacaagca caatcgattc gtgaacattt atttaaaatg gaccaatcgt
     2701 ttattgagaa aataaatcta ccggaaggaa cagcattatt tacaaaagag gaattaatta
     2761 ctacagttac gcaattaaaa aaacaattcg atactgaaga tggtggattt ggctcagcac
     2821 ctaaatttcc gggcagcatg gccttgctct ttttgttgag atataatttt tttcttaaag
     2881 atgaagctgt tcaacaacat atccatttat cgttaaataa aatgatgttt ggcggaattt
     2941 acgaccacat tggcggtggc tttgcccgtt atactgtcga caaaaaatgg atgataccac
     3001 atttcgaaaa aatgttgtac gacaatgctt tattaattag tttgtatgct gaagcttatc
     3061 gggaacaacc aaatgctaat tataaaagtg taattgaaaa tacgcttgct tttgttgagc
     3121 gtgagtggat gacatcagaa ggtggatttt atgcttctta tgatgccgac tcggaaggag
     3181 tggagggcaa attttataca tttacttaca acgaaattca gaccatatta aaagaaaatt
     3241 ttccccttgc cgaaaaagta tatcagataa aaaaagatgg caattgggaa catactaata
     3301 ttttatttcg caataacact aatgaagcgc ttgcagcaga aatggatata tcagttgctg
     3361 aattaacgca acagttaagt gccatcaata aacaattatt cgactaccga gaaaacagaa
     3421 ttaaacccgg cttagataat aaaataattt tatcgtggaa tgccatgatg tgttctgctt
//
LOCUS       DAORKW010000405         3478 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1476_length_3478_cov_4.876132, whole genome shotgun sequence.
ACCESSION   DAORKW010000405 DAORKW010000000
VERSION     DAORKW010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1476_length_3478_cov_4.876132"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..418)
                     /locus_tag="PLB46_13235"
     CDS             complement(50..418)
                     /locus_tag="PLB46_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRF77534.1"
                     /translation="MRAVLLTFSIIASLNAFSQAPYKLQGTIEPGTWTVLSDMNESRN
                     GHTTTYLSDGKVLVTGGYDGTQNLATAEIYDPAADTWTTTGNMDSIRFSHTATLLDNG
                     KVLIIGGWNGGVFYYKYQHF"
     gene            complement(577..1722)
                     /locus_tag="PLB46_13240"
     CDS             complement(577..1722)
                     /locus_tag="PLB46_13240"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HRF77535.1"
                     /translation="MIKSLCIIVFITISVTLAAQTPAPVNTFWDKIKFNADLRTRTEY
                     DWMNITAAGDTNDNRLRLRTRARFGFTYQYNDKVEIGMRLRAGNAADQQSPHVSFGSP
                     GEFAPIGLAFDKAYIKVNHKGLWATTGKNTYPFYYHNELFWDEDVTPEGIAAGYDWKI
                     GNTTHVKPTAGFFFISSTGKPFDTDVTLKAAQLHVNVAKNKSEINIASGIFSINDLPN
                     LPDGTGTFKIDYTMVVSNLKYTYKSKIPIAVTADIMLNLEDYTDSTIVANQHQDQTTG
                     YVVGAELGQLKDKKNYMLGVYYAHIEKYSVVDYFAQDDWLRWGYSGATGTRSSNFEGV
                     ELRAGYAFGPKFNVLARTYLCNGIVANTPQSEIETANRFRLDLNIGF"
     gene            complement(1768..3087)
                     /locus_tag="PLB46_13245"
     CDS             complement(1768..3087)
                     /locus_tag="PLB46_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77536.1"
                     /translation="MKKLFLMYGMLCLASNLIAQTTLNNTPSKAVSVSIRSMNDEQYP
                     DNNSIGFRSTQVGIYTHNEIQFSPTENGKFDIQITPGNGKSDTILLSGIDLKEMIPSI
                     PVYVQQNEYLTYLALLNQEWNRIQVKFNNQQFVTTGPGDEDKIISRVDIANNCLGKGD
                     WEIIFFTKENDKEVLYFQCWFTFPDELYNQLFKMRNGIEITTFNNMLKNYGNRGGEYV
                     DLSVLRTVNGSQEIPFQNLNNELYPLKGERQTKAKNIIYPVNYKNINSFLNDSTQFAT
                     FASPGMYTKTQPRATQLSKFKALTKVMYAQTISTNTAKTPTTEIKLLYNAEGKTVQYI
                     IGGIIMDKIPVLSTDNLHNGYQRPMGIGNHSFYSAYNDILSNSSRENPYYGLLLDENG
                     LWLDSHTIGTDGPLLFREEGNPNKLHILLLSFERHAFVGHYVIAIPQ"
     gene            complement(3263..>3478)
                     /locus_tag="PLB46_13250"
     CDS             complement(3263..>3478)
                     /locus_tag="PLB46_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRF77537.1"
                     /translation="GADSNAGKFIFDADLTVPTAIIMGSEDEGISALSRKYLTNTVKI
                     PLQNNFDSYNVSVAAGMILYEVMRQRM"
BASE COUNT         1087 a          630 c          593 g         1168 t
ORIGIN      
        1 gcctgtatta catgtccgca cgatgatata tgttccggtt gcctggatat taaaaatgtt
       61 gatatttata ataaaaaact ccgccattcc atccaccgat gattaaaact tttccattgt
      121 ctaacaaagt agcagtatgc gaaaatcgta tactatccat gtttccggta gtagtccatg
      181 tatcagcagc cggatcgtaa atctctgcag tggcaaggtt ttgtgtgccg tcgtaaccgc
      241 ctgttaccaa tactttccca tcagataaat aggtggtggt atggccgtta cgcgattcat
      301 tcatatcgct caaaacagtc catgtaccgg gttcaatagt gccttgtaat ttgtaaggag
      361 cttgagaaaa ggcatttagg gaagcaatta tgctaaaagt tagtaatact gcacgcatac
      421 ttatgaagtt tgaagcaaaa ttaataattt aaataaatgc aggtatacca tttaaaaccg
      481 gcgtacaatt aaaaaggatg aagatttaaa aaatcatcat cctttttgaa attgtttaaa
      541 atggataaaa agcaacagcg gctaatcaat accctgtcag aatccaatat tcaaatctaa
      601 tcgaaaccgg tttgctgttt cgatttcaga ttgtggtgta ttagccacaa ttccattaca
      661 caaataagtt cttgccaaca cattaaattt tggcccaaat gcataacctg ctctaagttc
      721 cacaccttca aaattgctgc ttcgtgttcc tgtagcacct gaatagcccc atcgcaacca
      781 atcatcttgt gcaaaataat caactacact atatttttca atatgcgcat aatacacgcc
      841 taacatataa ttttttttat cttttaattg gccaagttca gcaccaacta cataacctgt
      901 tgtttggtcc tgatgttgat tggcaacaat agttgagtcg gtataatctt caagatttaa
      961 catgatatcc gcagtaacag caatcggaat ttttgattta taagtatatt ttaaattact
     1021 tacaaccatc gtataatcaa ttttaaatgt gccagtgcca tccggtaaat tgggtaagtc
     1081 gttaatacta aaaatacctg atgctatatt tatttctgat ttatttttag caacattaac
     1141 atgcaattgt gctgctttta aagttacatc tgtatcgaaa ggtttaccgg tagaagaaat
     1201 aaagaaaaat ccggcagtcg gtttcacatg tgtagtatta ccgattttcc aatcataacc
     1261 ggctgctatg ccttcgggtg ttacatcttc atcccaaaat aattcgttgt ggtaataaaa
     1321 tggataagta tttttacctg ttgttgccca taagccttta tgatttactt ttatgtaggc
     1381 tttatcaaaa gctaatccta taggagcaaa ttctcccggt gagccaaacg atacgtgtgg
     1441 tgattgctga tcagcagcgt ttccggcgcg gagtcgcatt ccaatttcaa ccttgtcgtt
     1501 atattgataa gtaaatccaa atcttgcacg agtacgcaac ctcaatctgt tgtcgttggt
     1561 atcaccggct gctgtaatat tcatccagtc atattcagtt cgtgttcgta aatcagcatt
     1621 aaatttaatt ttatcccaaa aggtatttac tggtgccggc gtttgagcgg caagtgttac
     1681 tgaaattgta atgaatacaa ttatgcataa tgatttaatc ataaaccaat ttttttttga
     1741 agtgaaataa atgaaaagtg aaacctgtta ttgtggaatt gctattacat aatgtcccac
     1801 aaatgcatgg cgttcaaaag acagtaataa aatgtgcaat ttattaggat taccttcttc
     1861 cctaaataac aatggaccat cagtgccaat ggtatgacta tccagccaca aaccattttc
     1921 atctaatagg aggccataat atggattttc gcgtgacgaa tttgaaagaa tatcgttata
     1981 tgcactataa aaagaatgat ttccaatacc cattgggcgt tgatatccat tatggagatt
     2041 atcagttgaa agaacaggaa ttttatccat tataatacct ccaataatgt attgaacagt
     2101 tttcccctca gcattatata acagtttaat ttctgttgtc ggggttttag cagtattagt
     2161 tgaaatagtt tgcgcataca tcaccttggt taatgctttg aacttactta attgtgtagc
     2221 acgcggctgt gttttagtat acataccagg tgatgcaaat gttgcaaatt gcgtagagtc
     2281 attaaggaaa ctatttatat tcttataatt gaccgggtaa ataatatttt tagccttcgt
     2341 ttgacgttca ccttttaaag ggtatagctc attgttgagg ttttgaaatg gtatttcctg
     2401 tgaaccatta acagtgcgta aaacgcttaa atctacatat tccccacctc tgttgccgta
     2461 attttttaac atgttattaa atgtggttat ttcaatgcca ttgcgcattt taaataattg
     2521 attgtataat tcatcaggaa aggtaaacca acattggaag tataatactt ccttatcgtt
     2581 ttcttttgta aaaaaaataa tttcccaatc tcctttacct aaacaattat tagcaatatc
     2641 aactctgctg attattttat cttcgtcgcc tggaccggta gtaacaaact gttggttgtt
     2701 aaattttacc tgaattcggt tccattcctg atttaacaat gctaaataag tgaggtattc
     2761 attttgttgt acataaaccg gtatagaagg aatcatttct tttaaatcaa ttccacttaa
     2821 aagaatggta tcacttttac cattacccgg cgtaatttga atgtcaaatt tgccgttttc
     2881 ggtaggggaa aattgaattt cgttatgcgt ataaatacca acctgtgtac tcctgaaacc
     2941 aatactgtta ttgtccggat attgctcatc attcatactc cgaatagata cactaacagc
     3001 ttttgaaggt gtattattta gtgttgtttg agcgattaaa ttggacgcta aacatagcat
     3061 gccatacatt aaaaagagtt ttttcatttg aagcctgttt tgagggtaaa aataaaccta
     3121 tcaatcccgg caactggtaa ttatttgtaa atgactttaa acgtttttca aatgtttagg
     3181 gaaaatagat aacaggagta cacttttcag gacttacttg cagcgaccaa ttgaataata
     3241 ttgaaattac tttgtataat tatcacatgc gttggcgcat aacttcgtat aaaatcatgc
     3301 ctgcagcaac agaaacatta tatgaatcga aattattttg tagtggaatt ttcactgtat
     3361 tggtgagata ttttctcgat aacgcactta ttccttcatc ttccgaaccc attatgatag
     3421 cagttggaac agtcaggtcg gcatcaaaaa tgaatttccc cgcattacta tccgcacc
//
LOCUS       DAORKW010000406         3452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1494_length_3452_cov_5.481013, whole genome shotgun sequence.
ACCESSION   DAORKW010000406 DAORKW010000000
VERSION     DAORKW010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1494_length_3452_cov_5.481013"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PLB46_13255"
     CDS             <1..1195
                     /locus_tag="PLB46_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF77538.1"
                     /translation="VDRLGNIQWQNDIGGNVSDVLTDIHQTADEGFMVGGYSSSDISG
                     DKTEVNIGFADYWIIKLDNIGNILWQKTFGGWSDDNLTTCSNTVDSGQILGGTSLSPA
                     SGDKTEDNNGYQDFWIIKLFPDTCEKTAFYLDTDNDGFGNSDDSIFACFAPFGYVANQ
                     GDCNDTIGEINPTAIEICNVIDDNCDGGIDEGLLLSVFYADNDNDGYGNSLIDTTTCL
                     TEIANYVSDSSDCDDSNNLIHAMITYYADADSDSYGDVFNPAAFCNTFAPPGYVTDST
                     DCDDTNSDINPGISEILNGLDDNCNDSIDEGITSIYNLTIPDFRIYPNPNNGFFIIKL
                     MHVDQPYILIKIYDITGRIVYSNNFHSDSTISIKLINFVTGIYEIQISFGNNSVKKPI
                     IVQSL"
     gene            1206..3209
                     /locus_tag="PLB46_13260"
     CDS             1206..3209
                     /locus_tag="PLB46_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77539.1"
                     /translation="MKYIFFFLSILGILQVHAQVTCTTTDPILINDVNSGNTSVNYTL
                     KLDGISGYSLSNQLLGSNSSQDALEWKTLAPGTSGSDFNIAFSAGAIIFNLPDAGSST
                     RGVVNTSGSGQTFAGPKTFTSDLSISGSTTNLFLTDGVDQTTPSLAFTSNPGSGFSYT
                     NSTKTLFLSLNGVYGYQWLSGEFDMKNCANFIAGAGKLDINITHDADSESPKQDGGVS
                     VSTYSGTETKTCTFGGRTANGTESEPASTVLDQAMVEFVGKGYVLCPFEDPEDCDDYG
                     FEHTNRGVFGVYASETHSLSSQGAYCAIQTTRIGTTNDHFSFLVDAAGNGGLTYNKII
                     PIAYPDLWSDGTDPEDDYDSRFFTVESETNTADVGLFLQNNNALKGLNIWLDNDQDIS
                     YIDNIRNSNGAAIKFRLKTAGSSVTAMTINAHGSNIGGGTPASGETFSVGSNRDLIVN
                     SSGKLSKYQNTTISDGELLIGKTTGSTFEKTTLTSGTGISVSNGSGAITINASSNYTS
                     TAFKTIANTTIETTLLASGTGSLTINANTLTVGKTYIIKAYGYVSTDASAPNWTVKAK
                     LGSTEIASTTGAAPASLSNRRVEITVIFTCYTIGTSGTIAAQGSFGYNSNATNGFTRE
                     MVNTSTITLNTETNQTIDLTFTWSAADTDNTITITNATIEAMK"
BASE COUNT         1141 a          609 c          663 g         1039 t
ORIGIN      
        1 agtcgacaga ttaggcaata ttcaatggca aaatgatatt gggggaaatg tatccgatgt
       61 attaacagac attcatcaaa ctgctgatga agggtttatg gtaggtggtt atagtagttc
      121 agatatttca ggggataaga cagaagttaa tattggtttt gctgattatt ggataatcaa
      181 actggataat attggtaata tattatggca aaaaacgttt ggaggctggt cagatgataa
      241 tcttacaacc tgctcaaata cagtcgatag cggacagatt ctcggaggca cctctttatc
      301 ccctgcttcc ggtgataaga cggaagataa caatgggtat caggattttt ggataattaa
      361 attattcccc gacacttgcg aaaaaacagc tttttattta gataccgata atgatggatt
      421 cggcaattct gatgatagca tatttgcatg ttttgctcct tttggatatg tggcgaatca
      481 aggcgattgt aatgatacga taggagagat aaaccctact gcaatagaaa tttgtaatgt
      541 tattgatgat aattgtgatg gtggtatcga tgaaggcttg ctcttatctg tattttatgc
      601 agataacgat aatgatggct atggcaattc attaattgat acaacaacat gcttaaccga
      661 gattgccaat tatgtttccg attcatctga ttgcgatgat tccaataatt taattcatgc
      721 aatgattacc tattatgcag atgcggacag tgattcttat ggcgatgtat ttaatccggc
      781 agctttttgt aatacttttg caccaccagg ttatgtaaca gatagcacgg attgcgatga
      841 cacaaattca gacattaatc cgggtatatc agaaattctt aacggcttgg atgataattg
      901 taatgacagt attgacgaag gtataacttc aatttataat ttaactatac cggattttcg
      961 aatttatcca aatccaaaca atggtttttt tataatcaaa ttaatgcatg tagatcagcc
     1021 gtatatactc atcaaaatat atgatataac aggacggatt gtatattcta acaattttca
     1081 tagtgattcc actatttcca taaagttaat aaattttgta actggaattt acgaaataca
     1141 aatatcattt ggtaacaatt ctgtgaaaaa accaattatt gttcaaagtc tataaatcaa
     1201 ttaatatgaa atacatcttt ttttttctca gcattcttgg tatactgcaa gttcatgccc
     1261 aagttacatg tactacaaca gatccaattc taataaatga tgttaattct ggaaacacat
     1321 cagtaaatta tacgctcaaa cttgacggga tatcaggtta ttcactatct aatcaattac
     1381 ttggttcaaa ctcatctcaa gatgcattag aatggaagac cttagctcca ggtactagtg
     1441 ggtcagattt taatattgca ttctcagccg gcgcaatcat attcaatctt cctgatgcag
     1501 gatcttcaac aagaggggtg gttaatacat ctggatcggg tcagacattt gccgggccaa
     1561 aaacctttac aagtgattta tccatatcag gatcaaccac gaacttattt ttaaccgatg
     1621 gggttgatca aaccacacca tccttagctt ttacttcaaa tcccggcagc ggttttagtt
     1681 atacaaattc aactaaaacg ttgtttttat cactaaatgg ggtttatggt tatcaatggc
     1741 tcagcggaga atttgatatg aaaaactgtg cgaattttat agctggtgca ggcaaacttg
     1801 atattaatat tactcatgat gccgattctg aatctcctaa gcaggatggt ggtgttagtg
     1861 tttcaactta ttcaggcacg gaaacaaaaa catgcacatt tggaggccga actgcaaatg
     1921 gaaccgaaag tgagcctgca agtactgtac ttgatcaagc aatggttgaa tttgtaggga
     1981 aaggctatgt gctttgcccc tttgaagacc cagaggattg tgatgactat ggttttgaac
     2041 atacaaatcg tggtgtattt ggtgtatatg cctcagaaac gcattcccta agttcccaag
     2101 gtgcatattg tgcaatccaa acgaccagga taggaacaac aaatgatcat ttcagttttt
     2161 tggtagatgc tgcaggaaat ggtggcttaa catacaataa aatcatacca atcgcatatc
     2221 ctgatttatg gagcgatggc actgatccag aagatgatta tgacagtcgt tttttcacag
     2281 tagaatcgga aacaaataca gcagatgttg gattatttct acaaaacaac aatgcgttaa
     2341 agggattaaa tatttggcta gacaacgacc aagatatatc ctatattgac aatattagaa
     2401 attcaaatgg cgctgcgatt aaattcagat tgaaaactgc aggctcttct gttaccgcaa
     2461 tgactataaa tgcacatgga tctaatattg gcggaggaac tcctgcttca ggagaaacct
     2521 tttctgtagg gtctaatcgt gatttgattg ttaattcatc cgggaaatta tctaagtatc
     2581 agaatacaac aatttctgac ggtgaattat tgatcggaaa aacaacaggc agtacatttg
     2641 aaaaaacaac acttacttct ggcactggta tttccgtaag taatggttca ggtgcaatta
     2701 cgataaacgc cagctcaaac tatacgtcta ccgcatttaa aactattgcc aatacaacta
     2761 tagaaacaac tttattggca tcaggaaccg gatcattaac cattaacgct aataccttga
     2821 cagttgggaa aacctacata ataaaggcat atggttatgt gagcactgat gcatctgctc
     2881 ccaactggac agtgaaagct aaattaggat caacagaaat tgcatctact acgggcgcag
     2941 cacctgcttc actgagtaac cggcgcgttg aaataacagt tatatttacc tgctacacaa
     3001 tcggaacgtc aggcaccata gcagctcagg gaagttttgg ttataattca aatgcaacga
     3061 atggatttac aagggaaatg gtgaatacat ctaccataac attaaatact gaaacaaacc
     3121 aaacaattga tttaacattt acctggtcag ctgctgatac agacaatacc atcactatta
     3181 caaatgcaac gattgaggca atgaaataat tttttgagct ttgaaataaa ggcgaactga
     3241 atttcaaacc gagtactcca ccactggcta taacagcaca tttgcaatag gcggggattc
     3301 atgctttgca tacagttttg tggttaaaga aagttcagtt ctccggataa atatttgtgc
     3361 tgaaaatccc gcccatcgca aatctgcaaa ccgttatgcc tcatgctatg acgaaacaac
     3421 ggattactat aaaacaataa ttactggaaa at
//
LOCUS       DAORKW010000407         3431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1508_length_3431_cov_4.716825, whole genome shotgun sequence.
ACCESSION   DAORKW010000407 DAORKW010000000
VERSION     DAORKW010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1508_length_3431_cov_4.716825"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLB46_13265"
     CDS             <1..411
                     /locus_tag="PLB46_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HRF77540.1"
                     /translation="KKELELFHQDSLEPLFIDPVDTVICDLPVGYYPNDEGAEAFELK
                     ADKFKPDHLGQLNFYLEALDRDVKKTNENPSLGILLCKDKDSEVVEYALSRSLSPTMV
                     AEYKTQLPDKKLLQQKLHELFDNETEVKQNNLDQ"
     gene            complement(556..1062)
                     /gene="folK"
                     /locus_tag="PLB46_13270"
     CDS             complement(556..1062)
                     /gene="folK"
                     /locus_tag="PLB46_13270"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRF77541.1"
                     /translation="MMTEHIAYLLLGTNLGNRTENLSKATAAIRMFLGRVDSQSHIYE
                     TEPWGKPHQPNFYNQAIKITTPCSPLETLHLIKQVEFLLGRDNAEKWAPRIIDIDILF
                     FDKFAIESPVLTIPHAHIAKRKFTLEPLKEIAPDLVHPVLQKTITELYLSCSDELTVK
                     AVDAVYQF"
     gene            1155..2930
                     /gene="sppA"
                     /locus_tag="PLB46_13275"
     CDS             1155..2930
                     /gene="sppA"
                     /locus_tag="PLB46_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRF77542.1"
                     /translation="MKEFFRSFFASLLAFFVFCLLFFFLIAGIVGAMSKSFEKEKAKV
                     SENTVLKINTNYAIPEQTQSGVPAGLGLLGFDGNQGIGLNDILANIKNAETDEKIKGI
                     YIELGLNANSYATIQEIRDALEDFKKNSGKFIIAYGEVVSQSSFYLGSVADKIYLNPA
                     GAIDFKGLSAQLTFYKGTLDKLGVKTQIFYDGKFKGATEPYRMEKMSDENRLQLEEFL
                     NGLFDENLKEINENRNLSVEKYKLIADSLLAVYPTAAKQVGIVDDLKYYDEVLAEIKT
                     RCGLNTDDELKFVGQKDYRTSFTIEAPESSDGKIAVVYADGTIIDGSGENGYLGSKNF
                     TEMMQKVRKDKDIKAVVLRVNSPGGSAIASDVMWREIEITKKEKPVYVSMGDYAASGG
                     YMISCNADKIYAQPNTLTGSIGVFLIVPEISDFMNNKIGITFDTVNTSAHSDFPSITR
                     QFSPKEQLILQNGVDSTYLHFKQMVATGRNMTVDQVEAIAQGRIWTGVKAKEIGLVDE
                     IGGIDEAIKGAKEKAGLKSYTIVEFPEQESTIIDDIILSLTEETKAKVQAEQLGVLYP
                     HYLAVKDLINRPVMQTRIPYNITIK"
     gene            3065..>3431
                     /locus_tag="PLB46_13280"
     CDS             3065..>3431
                     /locus_tag="PLB46_13280"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF77543.1"
                     /translation="MRLLTGIITMLIFSLSASAQNYGEISGQLKDLEEQFGIPQARMQ
                     LTQDGATVMELVTDNNGGFVFKPLNPGIYDISILAFGYDTAVYKSIEVSSGGFNFQVF
                     EIIAGVELIETIIRPPLVDN"
BASE COUNT         1149 a          568 c          697 g         1017 t
ORIGIN      
        1 aaaaaagagc ttgagctttt ccaccaagac agccttgagc cgttgtttat cgatccagtt
       61 gacacggtca tttgtgatct gccagtcggc tattatccga atgacgaagg agcagaagcg
      121 tttgagctca aagctgacaa gtttaagcca gaccatttgg ggcaactgaa tttttattta
      181 gaagcattgg atagggatgt gaaaaaaact aatgaaaacc cgagtctcgg tattttactt
      241 tgtaaggaca aggacagtga ggttgtagaa tacgctttaa gccgaagtct ttcaccaaca
      301 atggttgcgg aatataaaac tcaactgcct gacaagaagc ttttacagca aaaattacac
      361 gaactatttg ataatgagac agaagtgaaa caaaacaact tagatcaata gtttttgctt
      421 gcttcaggtt aactacattt agatttaaag aattccctct atattgtttt caagaatgct
      481 ataaaatcag ttcaatagcc tgaatcaatg aaacaaccgt tctgtttcaa aaataattat
      541 taggaggaca gcaccttaaa attgatacac cgcatcaacc gccttaaccg tcagttcatc
      601 actacagctc aaataaagct cggtgattgt cttttggagt accggatgca ctaaatccgg
      661 agcaatctct ttaagtggct ctaatgtgaa cttacgcttg gcaatatgtg catgcggaat
      721 agttaacacc ggcgattcaa tagcgaattt atcaaaaaag agaatatcga tatcgattat
      781 cctgggtgcc cacttttcag cattatctct tcccaacaaa aactccacct gtttaataag
      841 atgtaaagtt tcaagtggtg agcaaggcgt agttatttta atggcttgat tgtagaagtt
      901 gggttggtgt ggttttcccc aaggttcggt ttcgtaaata tgggactggc tatctactcg
      961 accaagaaac atccgaatgg cggctgttgc cttcgaaaga ttctccgtgc ggtttcctaa
     1021 atttgtgccc aacaataaat aggctatatg ttccgtcatc attggctata gaggagttga
     1081 ctccgaaagt taaacgtttc aagtggaaag ttagtgcata ttttataaaa aaaaacaaaa
     1141 attgtaagaa aattatgaaa gaattcttca gatcattctt tgccagcctg ctcgcgtttt
     1201 tcgtgttttg cctgctgttt ttcttcctga ttgcaggaat agtaggtgct atgtcgaaaa
     1261 gtttcgaaaa agaaaaggca aaagtcagcg aaaataccgt gttgaaaatt aataccaact
     1321 acgccattcc tgaacaaacg cagtccggtg tacccgcagg tttaggctta ttaggttttg
     1381 atggtaacca ggggattggc ttaaacgaca tcctggctaa tattaaaaac gcagaaacag
     1441 atgaaaaaat aaaagggatt tatattgaat taggattaaa tgccaacagt tatgctacga
     1501 tacaagaaat tcgtgatgct ttggaagatt ttaaaaagaa cagcggcaaa tttatcatcg
     1561 cttatggaga agttgtttct caaagtagtt tttacctcgg aagtgttgca gataaaattt
     1621 atttaaatcc tgctggtgca atcgacttca aaggattaag tgcacaactt actttttaca
     1681 aaggcacatt agataaatta ggtgttaaaa cacaaatttt ttacgacggt aaatttaaag
     1741 gtgcaacaga accttatcgc atggaaaaaa tgagcgatga aaacagattg caactggaag
     1801 aatttttaaa tggattattt gatgaaaatc tgaaagaaat aaatgaaaac agaaatttat
     1861 cagtagaaaa atataaatta attgccgata gtttattagc tgtttatcct actgctgcca
     1921 aacaagttgg tattgttgat gatttaaaat attacgatga agtattggca gaaataaaaa
     1981 cacgttgcgg attaaataca gatgatgaat taaaatttgt cggtcaaaaa gattatcgca
     2041 cctcgtttac tattgaagca cctgaatctt cagatggcaa gatagctgtg gtgtatgccg
     2101 atggaactat aattgacggc tccggcgaaa atggttatct cggttctaaa aattttacgg
     2161 agatgatgca aaaagtgcgc aaggataaag acattaaagc agtggtatta cgggtaaatt
     2221 ctccgggtgg ttcggctatt gcgagtgatg taatgtggag agaaattgaa atcaccaaaa
     2281 aagaaaaacc ggtatatgtt tccatgggcg attatgcagc cagtggtggt tatatgattt
     2341 cctgtaatgc tgataaaatt tatgcgcaac ccaacacatt aacaggaagt attggcgtgt
     2401 ttttaatcgt gcctgaaata tcagatttta tgaataataa aattggtatc actttcgata
     2461 cggtaaacac ttcagcacat tccgattttc cttccattac acgacagttt tcacctaaag
     2521 aacaacttat tttacaaaat ggtgtagact caacttattt gcattttaaa caaatggtgg
     2581 ctaccggaag aaatatgact gttgatcagg ttgaagccat tgctcaaggt agaatttgga
     2641 ccggtgtaaa agcaaaagaa attggcttgg tggatgaaat tggaggcatt gatgaagcca
     2701 ttaaaggtgc aaaagaaaaa gcaggtttaa aatcatatac tattgtggaa ttcccggaac
     2761 aggaatccac cataattgat gacattattt tgagtttaac ggaagaaaca aaagcaaaag
     2821 tacaagcgga acaattaggt gttttatacc cgcattatct ggctgtaaaa gatttaatca
     2881 accgaccagt aatgcagacg cgtataccgt ataacattac gataaagtaa cttgattcgt
     2941 atcagtgctg tttgtcatca aacagtcaat gttatgtcac aggcaattag attgcaatcg
     3001 tgtgacataa catttttttt atgcctttat ttttggctta aacattcact ttaaaacatc
     3061 aaatatgagg ctattaacag gaattattac catgctgatt ttttctctga gcgcatctgc
     3121 ccaaaactat ggcgaaattt caggacaatt gaaagattta gaagagcagt tcggtatccc
     3181 gcaggctaga atgcaattaa cacaagatgg tgctacggtt atggaactgg tgacagataa
     3241 taatggcgga tttgttttta aaccgttaaa tccgggtatt tatgacatat ctattctcgc
     3301 attcggatat gatacagctg tatataaatc aattgaagtt tcttcaggcg gatttaattt
     3361 tcaggttttt gaaattattg caggcgttga attgatagag accattattc gcccaccatt
     3421 agtagataac c
//
LOCUS       DAORKW010000408         3417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1514_length_3417_cov_4.751933, whole genome shotgun sequence.
ACCESSION   DAORKW010000408 DAORKW010000000
VERSION     DAORKW010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1514_length_3417_cov_4.751933"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLB46_13285"
     CDS             complement(<1..183)
                     /locus_tag="PLB46_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77544.1"
                     /translation="MKNLFYIAGLCILLASCTNYGTKIDFNGDEVYYKDPVTEQEATQ
                     VGEFLKSYQYFTGQGFT"
     gene            393..1829
                     /locus_tag="PLB46_13290"
     CDS             393..1829
                     /locus_tag="PLB46_13290"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRF77545.1"
                     /translation="MKQIVKSTFFITAGGALPLLSSLVLLIPYTNNLSTANYGALAIY
                     ISFALLVQILMNYAIDAYLSVHYYDYQDDDNKLKHFLSSVFGILLSLGGVFILVISLV
                     GYLIFPIIFKGNEISFFPYGLMSIITAFFNAWFRTYVNIQVFGEKPIKYFLFGLFNFL
                     VTIAISIYLINLYPYTLIGPMWGRLISGVLIFLLTFSFGLKEFGLKFDYKLLPSISAY
                     ATPVLIFSLLTWVLAYINNYILSGIMTVGDVGIYDFALKCTLGIEYAGLGIAGAFNPR
                     IYKNWKSKGELVSESHEENRYYHVFSAVNILIISTTIFIVPYLIRLFVNNEQYYESLA
                     YLPLLCAAFVFKSLHTIYLNPIMYFKRTNLLPTILVVTAVVQIISGIVLINLFGIWGA
                     VWSYFLVRPIQVLLLRYKAKAFFRFSFNPIKIFWVPMFYVVGVIVLCSTNLTEFEINA
                     AQLAFAIILILFAYKNEIKALPGMFKNV"
     gene            2309..>3417
                     /locus_tag="PLB46_13295"
     CDS             2309..>3417
                     /locus_tag="PLB46_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HRF77546.1"
                     /translation="MKKNLLLCFLLIRISILGAQENNIGSGIAVSLNGVTNNYLALGD
                     VFNDLNFPLTIEAWVNPTSWTPGGYSPIIATDNVGVGGSYYGFWFRFNSSGKLIFEIG
                     DGSGSGGSDRRGKVTTSAVALNKWTHVAVVATSVTDIKFYFNGVLQLSSSTDGGASNT
                     SIVHTVYPAIIGGHITPTTQHNFKGQIDEVRIWDTVRTEAELRENLCLKINEISTYLI
                     GYWNFNESYGDPFTFNLSEPTDTAFIVGSVTKVTSGAPIGDEVVYLYATDYAGLNLTL
                     NSAGGDKMKINKIGNNPYGIHLYRVNSAPYSTLGLVNHPNYYFGVFTADGATAAKYNS
                     NYIYSFANGVITPFNEEFATAFQRVDGSVATWTDF"
BASE COUNT         1036 a          496 c          636 g         1249 t
ORIGIN      
        1 ggtaaatccc tggccggtaa agtattgata tgactttagg aattcaccta cctgagtagc
       61 ttcctgctcg gtaaccgggt ctttataata aacctcatcg ccattgaagt cgattttggt
      121 tccgtaattg gtgcaagagg caagcaatat gcaaagaccg gcgatgtaaa agaggttttt
      181 cataatgtga tgtttaagcc aaagatataa attctccata aaggatttag tttttcattc
      241 aataaaatgc tactatgatt tgtcctgttt tataaaaaag ctcaaaattc gctggcagcc
      301 ttccggtatg tcacataata attatatagt gtgactttgc taattgccta ttttcgattc
      361 cttatgttta tttttgttct ccaacctaag gtttgaagca aatagttaaa tcaacgtttt
      421 ttattacagc cggaggagct ttgccgctgc tctctagtct tgtattgtta ataccatata
      481 ccaataatct gtcaactgca aattacggcg cacttgcgat ttatatttca tttgcattat
      541 tggttcaaat attaatgaat tatgctatag atgcatattt gtcggtacat tattacgatt
      601 accaagatga tgataataag cttaaacact ttttaagtag cgtgttcggg attttgttat
      661 cattaggagg tgtttttata ttagtgatta gtttagtagg ttatttgatt tttccaatca
      721 tatttaaagg aaatgagata tcttttttcc catatggttt aatgagcatc attacagcat
      781 tttttaatgc ttggtttaga acatatgtaa acattcaggt gtttggagaa aagccgataa
      841 aatatttttt atttggcttg tttaactttt tggttacaat agcgatctcc atttatttga
      901 ttaatttata tccatacaca cttataggcc ctatgtgggg taggttaatt tcaggtgttt
      961 tgattttttt attaactttt agttttggtt taaaggaatt tggattaaaa tttgattata
     1021 aactgttacc aagtataagc gcttatgcca caccagtgct tatttttagt cttcttacgt
     1081 gggtattggc ctatatcaat aattatatac tttctggaat aatgacagta ggtgatgttg
     1141 ggatttacga ttttgcgctg aaatgtacgt tgggaataga atatgcaggt ttgggaattg
     1201 caggcgcttt caatccacgg atttataaaa actggaaatc aaaaggtgaa ttagtaagcg
     1261 agagccatga ggaaaatagg tattatcatg tttttagtgc ggtaaatatc ctcataattt
     1321 cgaccaccat ttttatagtg ccttatttaa taagattgtt tgttaataat gagcaatatt
     1381 atgagagtct ggcataccta ccattacttt gtgcagcttt tgtatttaaa agcttgcata
     1441 ctatttactt aaatccaata atgtatttca aaagaacgaa tttactgcct acgatattgg
     1501 ttgttactgc tgtagttcaa ataatttcgg gtattgtttt aattaattta ttcgggattt
     1561 ggggagcagt atggagctat tttttagtta ggccaataca agttttattg ttgagataca
     1621 aagccaaagc attttttaga ttttcgttta acccaataaa aatattttgg gtaccaatgt
     1681 tttatgtggt gggtgttatt gttttgtgca gcacaaatct tactgaattt gaaataaatg
     1741 ctgctcaatt ggcatttgct ataatactca ttttatttgc ctacaaaaat gaaattaaag
     1801 ccttgcccgg aatgtttaaa aacgtttaat aggcgagtgc atattcttta ttgctaaata
     1861 gtgtagtcat cagtgttaaa ttaatctaaa ctcagaattg ttaagttttt cagggcattt
     1921 cctagactgc ctaggcttat tttcatggtt gatggcggca acaattatta tcataatata
     1981 ttgatagtca ataatttata aatcatagtg tgtgccctgc aaaatttatt gctttgaagg
     2041 caagtgataa tcccttattt ttttatattt ttagggttgc aaactttgac cccgttaata
     2101 aatataaaat attattgaaa acaaacgaat tacagccgac cctataatca attttacttt
     2161 tgtaaaataa cgttttatgc agataatgca ataccaatga atttatagca ttattggacg
     2221 taattataaa gaagcctcta ttaatttgtt ttcatcattt aaattttact ttttaactat
     2281 attaatattt aaccacaaat tacacacaat gaaaaagaac cttttattat gctttctttt
     2341 aattagaatt tcaattcttg gtgcacagga aaataatatt ggatcaggta tagcggtttc
     2401 attaaacggt gttacgaata actacttagc attaggcgac gtgttcaatg atttaaattt
     2461 tccgttaacg attgaagctt gggtaaatcc aacttcatgg acgccaggtg gatattctcc
     2521 aataattgct actgataatg ttggtgttgg aggaagctat tatggatttt ggttccgttt
     2581 taattcatct ggaaaattga tttttgaaat tggtgacggc tccggttctg gcgggtcgga
     2641 tagaagaggc aaggtgacaa catcggctgt agctttaaat aaatggactc acgtggcggt
     2701 tgttgcaaca tctgttactg atattaagtt ttactttaat ggtgtattgc agctttcaag
     2761 ttcaactgat ggcggagcat ctaacacatc gattgtgcat acggtttatc ccgcaattat
     2821 tggaggacat ataacaccta caacacagca taatttcaaa gggcaaattg atgaggttag
     2881 gatttgggat acagtgagaa cggaggcaga gttgcgagaa aatttgtgtc taaaaattaa
     2941 tgaaatttca acttatttaa taggttattg gaactttaat gaatcatatg gtgatccatt
     3001 tacttttaat ttaagtgaac ctaccgatac cgcttttata gttggtagtg ttacaaaagt
     3061 aacatccgga gctcctattg gagatgaagt tgtttacttg tatgcaactg attacgcagg
     3121 tttgaattta acattaaatt cagctggcgg tgataaaatg aaaattaata aaattggtaa
     3181 taacccttat ggtatacatt tgtatagagt taactcggcg ccgtattcca cacttgggtt
     3241 agtaaatcat cctaattatt actttggggt atttactgca gatggtgcaa cagctgcaaa
     3301 atacaattcc aattatatct attcatttgc aaacggggtt attacacctt ttaatgagga
     3361 atttgctaca gcattccaac gggttgatgg ttctgtagct acctggactg atttttt
//
LOCUS       DAORKW010000409         3390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1526_length_3390_cov_6.326537, whole genome shotgun sequence.
ACCESSION   DAORKW010000409 DAORKW010000000
VERSION     DAORKW010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_3390_cov_6.326537"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLB46_13300"
     CDS             complement(<1..303)
                     /locus_tag="PLB46_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRF77547.1"
                     /translation="MRKNISSGAKWEDIVGYSRAVIINNTIEVTGTVAVDADNNLVGG
                     DSAYEQTKFVIQKIEKVLQQCGAELKHVVRTRIFVTDISRWEEYGRAHGEFFKDIKP"
     gene            complement(305..1252)
                     /locus_tag="PLB46_13305"
     CDS             complement(305..1252)
                     /locus_tag="PLB46_13305"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRF77548.1"
                     /translation="MKLKNPISLKDCAALLNATYSGNGDALITGINEIHKVTSGDITF
                     VDFHKYYDKALNSAATFVIINKEVECPDGKGLIFSDDPFRDYVKLVKHFAPFTPSLKN
                     IADNAVIGEGTIIFPGVYIGNNVIIGKNCILHPNVVIYDNAIIGNDVIVHANTVLGAD
                     AFYFKRREKHFDKMESCGRVIIHDGVEIGACCTIDKGVSGDTIIGEQTKLDNHIHIGH
                     GVEIGKMCLFAGQVGIGGKTIIGNNVIAWGQVGISKTLHIGDNVVILAQSGVGKDLEA
                     GKTYFGSPASEARQKMKQMAAANNMLEIWETMRKKNADN"
     gene            1420..2511
                     /gene="gcvT"
                     /locus_tag="PLB46_13310"
     CDS             1420..2511
                     /gene="gcvT"
                     /locus_tag="PLB46_13310"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRF77549.1"
                     /translation="MKQTALTQLHKDLGAKMAEFAGYEMPIVYSGQVEEHHTVRNAVG
                     VFDVSHMGEFILKGEGAAALIQKVTSNDVYALSPGKAQYSCLPNENGGIVDDLLVYHL
                     EDGNYMLVVNASNIEKDWNHIMKYNTENVDMVNISDNTSLLAIQGPKAIEALQSLTDV
                     DLSTIAYYTFTKHQFAGVGNVIISATGYTGAGGFEIYFPKEHSLTIWNKIFEAGAAYG
                     IKPIGLAARDTLRLEMGFCLYGNDIDDTTSPLEAGLGWITKLKAANNEFVGKDKIVAG
                     KEAGLTRKLVGFEMIDRGIPRHDYPILDENGTVIGKVTSGTQSPSLNKAIGLGYVNNE
                     KSAVDSEIFIDIRQKPVKAKVVKIPFWNK"
     gene            2546..3274
                     /locus_tag="PLB46_13315"
     CDS             2546..3274
                     /locus_tag="PLB46_13315"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HRF77550.1"
                     /translation="MTNTQEDNRPILEVCLTPALWDQHDPKDKNVVVIDIIRATSTIA
                     SALHHGVKKMIPVETVEEANTWLEKGYLVAGERDGIKIPHFHFGNSPFEFMGEDVRGK
                     EIIHTTTNGTRCVLKSKGARNIVAGSFVNQDAIFDWIKKENRNTILFCAGWKDHYVMD
                     DALFAGAVVRELADDFRIIDDSTLSAKLLYKHVRENMLRTVKQATHFKRLQRLGIEND
                     IKFCMSNPQFDVVPIFDGVGFVKL"
BASE COUNT         1060 a          677 c          611 g         1042 t
ORIGIN      
        1 tggttttata tctttaaaaa actcgccatg ggctctgccg tattcttccc agcgtgatat
       61 atctgtaaca aaaatgcgag tgcgcactac atgttttaat tcggcaccac attgttgtaa
      121 aaccttttct attttttgaa tgacaaattt agtttgttca taagcagaat ctccaccaac
      181 taaattatta tctgcatcaa ccgcaactgt tcctgtaact tcaattgtat tattaataat
      241 aacagcgcgt gaatatccaa ctatatcctc ccattttgct cctgacgaaa tattttttct
      301 catattaatt atccgcgttt tttttgcgca tggtttccca tatttccagc atattatttg
      361 ccgctgccat ttgtttcatt ttttgacgcg cttcacttgc cggagaacca aaataggttt
      421 ttcctgcttc caaatctttt cctactccac tctgtgctaa aatgacaaca ttatcgccaa
      481 tatgtagtgt tttcgaaata ccaacctgtc cccacgcaat tacattatta ccgataattg
      541 ttttaccacc tataccaacc tgtccggcaa ataaacacat tttaccaatt tcaacaccgt
      601 ggccaatatg aatatgatta tccaattttg tctgttcacc aataatagta tctccactca
      661 cacctttatc aatagtgcaa catgcgccaa tttcaacacc gtcgtgaata attacgcgac
      721 cacaactttc cattttatca aaatgttttt cgcgtcgttt aaaataaaat gcatcagcac
      781 ctaaaactgt gttggcatga acaataacat cattaccaat aattgcattg tcataaatta
      841 ccacattagg atgcaggata caattttttc cgataattac attattgcca atatatactc
      901 cgggaaaaat tatagtgcct tcgccaataa ccgcattatc cgcaatattt tttaacgaag
      961 gtgtaaatgg tgcaaaatgt tttaccaatt ttacataatc gcgaaacgga tcatcgctaa
     1021 aaattaatcc tttcccatcg gggcattcaa cttctttatt aatgataaca aaagttgctg
     1081 cagagttaag agccttatcg tagtatttgt ggaaatcaac aaaagtgata tcaccactcg
     1141 ttactttatg aatttcattt attcccgtaa taagtgcatc accgtttcct gagtatgtag
     1201 catttaacaa tgcagcacaa tcttttaacg aaataggatt tttcaacttc atgctgcaaa
     1261 ggtaatcggt aaaaatacgc ggagtgtaaa agccctgaaa atttgtctgc ctattgcttc
     1321 cctatccctt atacaaatgt tcatcaagct atcccgcgaa tggcttatct ttgcgcctgc
     1381 ctttgccctt ttgagggtaa gagctaaact tcaaactata tgaaacaaac tgcgctcaca
     1441 caattacaca aagacctcgg cgccaaaatg gctgagtttg cgggctatga aatgcctatt
     1501 gtttattccg gacaagttga agaacaccat accgtgcgca atgctgttgg ggttttcgat
     1561 gtgagccata tgggtgaatt tattctaaaa ggcgaaggcg ctgctgcgtt aatccaaaaa
     1621 gtaacctcta acgatgtgta tgcattaagt cccggcaaag cgcaatacag ctgcctgcca
     1681 aacgaaaatg gcggcatcgt ggacgactta cttgtttacc acctcgaaga tggtaactat
     1741 atgttagtgg taaatgccag taacattgaa aaagactgga accatatcat gaaatacaat
     1801 accgaaaatg tagatatggt gaatatttct gataatacca gtttgctggc cattcaggga
     1861 ccaaaagcca ttgaggcgct tcaaagcctt actgatgttg acttgagtac aattgcttat
     1921 tacactttta ccaaacatca atttgccggt gtgggcaatg taattatatc tgcaactgga
     1981 tataccggtg caggtgggtt tgaaatttat ttcccaaaag aacattcctt aaccatttgg
     2041 aacaaaatat ttgaagcagg cgccgcttat ggcattaaac caattggcct tgctgctcgt
     2101 gataccttac gacttgaaat gggcttttgt ttgtatggca acgatattga cgataccaca
     2161 tctcctctgg aagcaggttt aggctggatt accaagctaa aagcggctaa taatgagttt
     2221 gtaggtaagg ataaaattgt agcaggtaaa gaagccggat taacaagaaa attggtaggc
     2281 tttgagatga tcgacagagg cattccacgc cacgattacc ctattttaga tgaaaatgga
     2341 accgtaattg gcaaagtaac ttcaggcact caaagtccga gtttgaacaa agcaataggc
     2401 ctaggatatg taaacaacga aaaatctgcc gttgattccg aaatttttat agatattcgc
     2461 caaaaacctg tgaaagccaa agttgtaaag attccatttt ggaataaata attggagcct
     2521 tttcaaatca attgctttca acattatgac caacacacaa gaagataatc gcccaatatt
     2581 agaggtttgt ttaacacctg ccctttggga tcaacatgac cccaaggata aaaacgtggt
     2641 agttattgat attattcgcg ctacctctac tattgcctct gcattacatc atggtgtaaa
     2701 aaaaatgatc ccggttgaaa ctgttgaaga agcaaatacc tggctcgaaa aaggttattt
     2761 agttgccggt gaacgcgatg gtattaaaat tccacatttc cattttggta attctccttt
     2821 cgaatttatg ggagaagatg tgagaggaaa agaaataatt cataccacca caaatggcac
     2881 gcgttgtgtt ctcaaaagta aaggtgcaag aaatattgtc gccggaagtt ttgttaatca
     2941 ggatgctatt ttcgactgga taaaaaaaga aaacagaaat actattttat tttgcgccgg
     3001 ctggaaagac cattacgtaa tggatgatgc gctttttgca ggtgctgttg tacgtgaatt
     3061 ggccgatgat tttagaatta tcgacgattc aactttaagt gcaaaattat tatacaaaca
     3121 cgttcgtgaa aacatgttgc gcacagtgaa acaagctact cactttaaac gtttgcaacg
     3181 attaggtata gaaaacgaca tcaagttttg tatgagcaat ccacagtttg atgtggtgcc
     3241 tatttttgat ggtgtgggtt ttgtaaaatt ataatcccac cttttaaaaa attactgccg
     3301 ctttttaaat tacgtatttt acttttcgtc atccgcactt aaaaagtgga tagactgaat
     3361 aaatcttgta gcattgttcg acaccaggat
//
LOCUS       DAORKW010000410         3388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1527_length_3388_cov_4.735974, whole genome shotgun sequence.
ACCESSION   DAORKW010000410 DAORKW010000000
VERSION     DAORKW010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1527_length_3388_cov_4.735974"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1237)
                     /locus_tag="PLB46_13320"
     CDS             complement(143..1237)
                     /locus_tag="PLB46_13320"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF77551.1"
                     /translation="MPETNRKVILIAPLDWGLGHATRCIPLIRACLSQGHMVIMASSG
                     RSLQLLKKEFPELTAIEIPSYDIYYQKKGSFIIKMIAQLGKVFRGIKREHLLTAEIVK
                     QYGVNLIIADNRYGIYHKDVFSVIITHQMMVKVPSFKIAEPFVYLWLQWHHKKFDTVW
                     IPDTAAENNISGDLSHKYPKPKHAQYIGILTRFRKPEQEVTKTNDILAIVSGPEPQRS
                     IFEEMIISQAKSIQQQVIIVRGISEVDTDEKIADNIRVISHLPGEQLFQLILQSETII
                     SRGGYSTLMDLALLNKKCIFVPTPGQTDQEYLVKALHKNNLVFGTTQDKFNLQEALTG
                     LANKSTFSIPSDVNAFIPYLNEAISYSEKK"
     gene            1310..1480
                     /locus_tag="PLB46_13325"
     CDS             1310..1480
                     /locus_tag="PLB46_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77552.1"
                     /translation="MKISKKILPLIAIAMLLLGSCGIFKGGDNCDCPKFGENTTQPAN
                     PDIENASAANLE"
     gene            1551..2066
                     /locus_tag="PLB46_13330"
     CDS             1551..2066
                     /locus_tag="PLB46_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRF77553.1"
                     /translation="MLTKKVETALNKQVEMEGYASNYYLSAASWCDSKGLVGCAGFLY
                     GHADEERMHMMKLFTYINDAGGHALAPNIKQPPLKFKNLKELFESVLEHELAVTKSIN
                     NLVDLCMGEKDHSSYNFLQWYVAEQHEEEKLFRTILDKINLLGPDDSRGLYWIDRELA
                     GIKPESSSEPK"
     gene            2199..3218
                     /locus_tag="PLB46_13335"
     CDS             2199..3218
                     /locus_tag="PLB46_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77554.1"
                     /translation="MLISAIAAFIIPFELFLFSYAVLGPLHYLTEISWLHKRQYFSPK
                     KRDYIPLVIITLLIAIPAIFSYLVQHLGPVNENGKIVVGEGTMRFLNFLYFENPSTAL
                     IFMGFAFALVMVLIKDSMQRIVAFIIVIILGILLRTNHFVDVLFAMFLPTLIHVFIFT
                     GAFILVGALKSKSTSGILSIVVFFGCAVSFFLIFPGAENYQISEYAKGSYDVSFFTLN
                     QSLFETFMHVPNPSADVIYNSSAGILITRFIAYAYTYHYLNWFSKTSVIKWHEVPRTW
                     LVTILVLWVSAVGLYLWNYEVGLMCLYFLSFLHVVLEFPLNFQSFKQIGEQVRLWFKG
                     GTART"
BASE COUNT         1020 a          627 c          624 g         1117 t
ORIGIN      
        1 ggtacttagc gacttcgtgg taaacaatgt attaattatt gttggaggta aagtagttct
       61 ttttcacaac aaaggctcaa aggggctcaa agtaacacga agaattttta tagcgtagaa
      121 acctgatgta aaaaacaata aattatttct tttcagaata acttattgct tcatttaaat
      181 aaggtataaa tgcatttaca tccgaaggaa tgctgaaggt tgatttattg gccaagccgg
      241 ttaacgcttc ctgtaaatta aatttatctt gtgtggtgcc aaatactaaa ttgtttttat
      301 gcaatgcctt taccagatat tcctgatctg tttgtccggg agttggcaca aatatgcatt
      361 ttttattgag caaagccagg tccattagtg tgctgtatcc acctctcgaa ataatagttt
      421 ctgattgtaa aatgagttga aacaattgtt cgcccggtaa atgtgaaata acccggatat
      481 tatccgcaat tttttcatct gtatcaactt cagaaatacc gcgtacaatt atcacctgtt
      541 gttggattga ctttgcttgc gagataatca tttcttcaaa aatacttcgc tgtggttccg
      601 gccctgaaac aatagccaga atatcattgg tttttgttac ttcctgttcc ggttttctga
      661 accgtgttaa aatgccaata tattgagcat gttttggctt tggatattta tgcgaaagat
      721 cacctgaaat attattttct gcagcagtgt cgggtatcca aacagtatca aactttttat
      781 gatgccattg cagccataaa tacacaaatg gttcagcaat tttaaaggat ggcactttta
      841 ccatcatttg atgggtgata ataacgctaa aaacatcttt atgataaata ccataacgat
      901 tatccgcaat tattaagtta accccatatt gtttaacaat ttcagccgta agtaaatgtt
      961 cgcgttttat tcccctgaac acctttccca gttgggcaat cattttaata ataaaactgc
     1021 cttttttctg ataataaata tcataagatg gaatttcaat ggcggttaat tccgggaact
     1081 ccttttttaa cagttgtagc gacctacccg aagaagccat gataaccatg tgtccctgac
     1141 tcaaacatgc cctaatgagc ggaatacaac gcgtagcgtg gcctaaaccc cagtcgagtg
     1201 gtgcaatgag gattacttta cggttcgttt caggcaaggc aatatggctt taaaaactgc
     1261 gtttatatgc taactgtaaa aatttgatta catttagagc cgcaaagcta tgaaaatcag
     1321 taaaaaaata cttcctctta tcgcaattgc catgctttta cttggttcat gcggcatttt
     1381 taaaggtggc gacaattgcg attgcccaaa atttggcgaa aacactacac aaccggctaa
     1441 tcccgatatc gaaaacgcct ccgctgctaa tttagaataa gtataaattt tctttttatt
     1501 gaactacaat tgtgtggtaa tgtttaacat tgcatacaaa tgatatactt atgctaacga
     1561 aaaaagtaga aacagcatta aataaacagg tggaaatgga aggttatgct tccaattatt
     1621 atttatctgc cgcttcatgg tgcgactcta aaggattggt cggatgtgcg ggatttttat
     1681 acggacatgc cgatgaggaa agaatgcaca tgatgaaact gtttacctac attaacgatg
     1741 caggtggaca cgcattggca cccaacatta aacaaccacc gctcaagttt aagaacctga
     1801 aagaattgtt tgaatcagta ttggaacatg aacttgctgt taccaaatca ataaataatc
     1861 tagttgattt atgtatgggt gagaaagacc attccagcta taatttcctt caatggtacg
     1921 ttgctgaaca gcacgaagaa gaaaaattgt tccgcactat tctcgataaa ataaatctcc
     1981 ttgggccgga cgattcaaga ggtttatatt ggatcgaccg cgaattagcc ggaattaagc
     2041 cggaaagtag ctcagaaccg aaataatttt acataaaatc tatttacctg cattgcgata
     2101 tcgtaatgcg ggtttttttt gtcgttttag aaacaaccta aattgtgtac atttgctgaa
     2161 tgacaactgc gcaggttaat tacctcaata taggcctcat gctgatatca gcaatagccg
     2221 catttattat tccattcgaa ttattcttgt tttcatatgc cgttttagga ccactgcatt
     2281 atttaaccga aatatcatgg ttgcataaac gacagtattt cagtccgaaa aaacgtgatt
     2341 atattccatt ggtaattatt accctgctta ttgccatacc cgcaatattt agttacctgg
     2401 tgcaacattt ggggccagtt aatgaaaacg ggaaaatagt ggtgggagaa ggaactatgc
     2461 gttttttgaa ttttttatat tttgaaaatc catctaccgc attaattttt atgggttttg
     2521 cattcgcact ggtaatggtg ttgataaaag attcaatgca gagaattgta gcatttatca
     2581 ttgttattat cctcggcata ttattgcgca ccaaccattt tgttgatgta ttatttgcca
     2641 tgttcctgcc tacattaatt catgtattta tttttacagg agcatttata ttagtcggcg
     2701 cacttaaaag taaaagcact tcaggaattt tatccattgt agtatttttt ggttgcgctg
     2761 ttagcttttt cctcattttt cccggagcag aaaattatca aatcagcgaa tacgctaaag
     2821 gcagttacga tgtttcgttt ttcaccctca accaatcgct gtttgaaact tttatgcatg
     2881 tgcccaatcc ttctgccgat gtaatttata attccagtgc cggaatttta attacccggt
     2941 ttatagcata tgcttatacc tatcattatc tcaactggtt ctctaaaact tctgttatca
     3001 aatggcatga agtgccgcgc acatggttgg ttactatttt agtgttatgg gtaagtgcag
     3061 taggtttata cttatggaat tacgaagtag gattaatgtg tttgtatttc ctcagcttct
     3121 tacacgtagt attagaattt ccattaaact tccagtcgtt taaacaaatt ggtgaacagg
     3181 tgcgcttatg gttcaaaggc ggaacagccc gaacttaagc caacgtgcct tcgcaataat
     3241 gtttgagaat ttttagcgca gtttccatat ctctgcccat cattttggga agaaaacggc
     3301 ctaataattt agcaaaacca ccgcgtttcg tttttcctgt aaccacccag ctaacatcaa
     3361 cggatttgtc gttgacagct tcaataat
//
LOCUS       DAORKW010000411         3375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1536_length_3375_cov_4.906024, whole genome shotgun sequence.
ACCESSION   DAORKW010000411 DAORKW010000000
VERSION     DAORKW010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1536_length_3375_cov_4.906024"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /gene="metG"
                     /locus_tag="PLB46_13340"
     CDS             <1..499
                     /gene="metG"
                     /locus_tag="PLB46_13340"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HRF77555.1"
                     /translation="VKLSTGAAVGSPFYLVEKVEDEVIEKKVAALEEIRKTRMEKEQS
                     IDNQQVEKTSPIKPTIAFEDFSKLDIRTGKITAAEKIQGADKLLKLNVDLGFEQRVIV
                     SGIALHFAPENLIGQQVAVLVNLAPRKMRGVESNGMILMAEDAEGKLHFVQTPDSAES
                     GMPIS"
     gene            660..3041
                     /locus_tag="PLB46_13345"
     CDS             660..3041
                     /locus_tag="PLB46_13345"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HRF77556.1"
                     /translation="MKNLSSLLLIVFTLSVAVSLQAQTPPVVPPVSSGTLESLGFDSE
                     EIDAIMGTTGTTTAVDAAGQIQDAQQAAVQNAMNQEATLAPNTSSIPPAVVAEVIADA
                     TDGGSAPAASLIWGHDFFRSGAVDLFDKVANQKVSDNYIIGEGDQLTVAIWGYAYYNY
                     NFTVNEEGYITTVEVGRIYLKGLTFSAAKQLLRQRFASAFDLTNSKFDVSLTYSKSIK
                     VNIVGEVMNPGSYNISSLNTAFNALVAAGGVTEIGSVRNIQIKRNGKVIKTLDVYQFL
                     MNPGATDDTYLEANDYIIVNGVGRIVEMQGEVNRPFKYELIKGENLNEVIAYAGGLRS
                     TAYKRNVTIFRYFENENIVMDINLDSLERERINYPLLDGDKIVFARIPEVIENIVTIQ
                     GSCRFPGNYQLTNGMRISDLIIKAKGLNYEAYTERAYLIRKDAKLNDVYVPFDLAEVM
                     ENPNSPFNFELNRFDVIDIFSKQEFKETFNVAINGAVKVPGTFPYYEKMTLKDLLYYS
                     GGLKVEAANSKIEISRIVNFNEASNNNEPTRVIVQSVAVSKELEIEDAANTFQLQPYD
                     QVFVRNTPEFEYQKNITILGEVKYPGVYTLTSRTETLADIVERAGGLTQWAYAEGATM
                     NRQDVTSTLVFLKKAMEDPSSKYNYVMREGDILTVPKAGDLVALKGEIKYPFVGDPGT
                     VVVPFEKGRSARHFIRKYGKNFDDDAKRSETYIVEPNGYVRRTHNFLFIHFYPRVKVK
                     GCQIVVPQKPEEKPEQETPEAPKEPFDWNTFATTLSAGILSFATIFVLLQRGN"
     gene            3076..>3375
                     /locus_tag="PLB46_13350"
     CDS             3076..>3375
                     /locus_tag="PLB46_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77557.1"
                     /translation="MQYDRPYNDEINIGDLFTKFGEYRRYVLKKWWVVLIFAILLAFL
                     LRFYAVWKKEQYISHADFAVKGTEGSSTSSLASLASSFGIGITTGTEFTNELFLGI"
BASE COUNT         1113 a          560 c          666 g         1036 t
ORIGIN      
        1 ggtgaaactt tctacaggtg cagctgtagg gtcacctttt tatttagtcg aaaaggttga
       61 ggatgaggta attgaaaaaa aagtagcagc attggaagaa atcagaaaaa cgagaatgga
      121 aaaggaacaa agtattgata atcagcaagt tgagaaaaca agtccaataa aaccgactat
      181 tgcgtttgaa gacttctcaa aattagatat ccgcacgggt aaaattactg cagcagaaaa
      241 aattcaaggt gccgataaac tgctcaaact caatgttgac ttaggttttg aacaaagggt
      301 aattgtttcg ggtattgccc tacattttgc acctgagaac cttataggcc agcaggtagc
      361 agtattggtt aacctcgctc cacgtaaaat gcgtggtgtt gaaagtaatg gtatgatatt
      421 gatggcagaa gacgctgaag gcaaattaca ttttgtccaa acccccgaca gcgctgagtc
      481 gggaatgcct attagttaaa catattaaat atttttcata agcaactagc cagtcttttg
      541 cttcgtctaa cggtggttgt ctgtcacaaa acaagcttgt aaaaacgcaa atttgttttg
      601 gtttgatagc actagattgc tttattaatc atatctttgc acgaaatttt aaaaaagtta
      661 tgaagaacct ttcttcgttg ttattgattg ttttcacgct tagcgtggct gtaagtttac
      721 aagcacaaac tcctccggtt gttccaccgg tatcttcagg tacattagaa tcacttggtt
      781 ttgattctga agaaatagat gccattatgg gcactacggg aactacaaca gcagtagatg
      841 cagccggtca aatacaagat gcacagcaag ctgctgttca aaatgccatg aatcaagagg
      901 caactcttgc tcctaataca tcttcaattc cacctgcagt tgttgcagaa gtgattgcag
      961 atgccactga cggaggatca gctccggcag cttctttaat ctggggacat gatttcttta
     1021 gatcaggtgc agtagattta ttcgacaaag tagctaacca aaaagtgagc gataattata
     1081 ttatcggcga aggcgatcag cttacagtag caatttgggg atatgcttac tataactaca
     1141 attttactgt aaacgaagaa ggatatatca ctactgttga agtaggtcgt atttatttaa
     1201 aaggtttaac ctttagtgca gcaaaacaat tattacgcca gcgttttgct tcagcgtttg
     1261 atttaaccaa ctcaaaattt gatgtaagtt taacttattc aaaaagcatc aaagtaaata
     1321 ttgttggtga ggtaatgaat ccgggttctt acaatatttc atcattaaat acagctttta
     1381 acgcacttgt tgcagcagga ggtgttacag aaattggttc tgttcgtaat attcaaatta
     1441 aacgcaacgg aaaagtgatt aaaactttag acgtttacca gtttttaatg aatcccggtg
     1501 ctacagatga tacttacctt gaagcgaacg attatattat tgtgaatggt gttggccgca
     1561 ttgttgaaat gcagggtgaa gtaaatcgtc cgtttaaata tgaattaatt aaaggtgaaa
     1621 acctgaatga agtaattgct tatgcaggtg gtcttcgttc aacagcatac aaacgcaacg
     1681 ttacaatttt cagatatttt gaaaatgaaa atatcgtaat ggatattaac cttgatagtc
     1741 tggaacgcga acgtattaac tatccgttat tagacggaga taaaatcgtt tttgctcgta
     1801 ttcctgaagt tattgaaaac attgtaacaa ttcagggttc ttgccgtttc cctggtaatt
     1861 atcagttaac caatggtatg cgtatttcag atttgattat taaagctaaa ggattaaatt
     1921 atgaagcata tacagagcgt gcttacttaa tcagaaaaga tgcaaaactt aacgatgtat
     1981 atgtgccatt tgatttagcg gaagttatgg aaaacccaaa ttcgccattt aacttcgaac
     2041 ttaatcgttt tgacgtaatt gacatcttct ctaaacaaga atttaaagaa acatttaatg
     2101 ttgctatcaa tggtgcggta aaagttcccg gaacttttcc ttactatgaa aaaatgacat
     2161 taaaggattt attatattat tcaggtggtt taaaagttga agctgcaaat agcaaaattg
     2221 aaatttcacg tattgtgaat tttaatgaag caagtaataa taatgaacct acaagagtta
     2281 ttgtgcagtc tgttgccgtt agcaaagaac tcgaaattga agatgctgct aatacattcc
     2341 aactgcaacc ttatgatcag gttttcgtgc gcaatacacc agaatttgaa tatcaaaaaa
     2401 atattaccat tttaggtgaa gtaaaatatc ctggtgttta taccttaaca tctagaaccg
     2461 aaactttagc tgatatcgtt gaacgcgccg gtggattaac acaatgggct tatgctgaag
     2521 gtgcaacaat gaaccgtcag gatgtaactt caacactggt attcttgaaa aaagcaatgg
     2581 aagatccttc ctctaaatat aactatgtaa tgcgtgaagg tgatatactt actgtaccta
     2641 aagccggcga cttagttgca ttaaaaggtg aaattaaata tccatttgtt ggcgatcctg
     2701 gaacagtggt tgtgccattt gaaaaaggta gaagtgctcg ccatttcatc agaaaatatg
     2761 gtaaaaactt tgacgatgat gctaaacgta gtgaaacata catcgttgaa ccaaacggat
     2821 atgttcgccg cacacataat ttcttattta tccattttta tccaagagta aaagtgaaag
     2881 gttgtcaaat tgttgttccg cagaaaccgg aagaaaaacc tgaacaggaa acaccagagg
     2941 caccaaaaga accatttgac tggaatactt ttgctaccac acttagcgca ggtatcctca
     3001 gctttgctac aatctttgta ttgttacaac gaggtaatta atctattaag ttaaaaggca
     3061 gcagaaaata aaattatgca atacgatcgt ccatataatg atgaaataaa tattggtgat
     3121 ctctttacca aatttggaga gtatagaaga tatgtattaa aaaaatggtg ggtagtgctc
     3181 atatttgcta tcctcctcgc ttttttactc cgcttttatg ctgtttggaa aaaagagcaa
     3241 tatatttctc atgctgattt cgctgttaag gggactgaag gttcatctac ctcttcatta
     3301 gcatctttag caagttcatt tggtattggt atcacaacag gtactgagtt tactaatgaa
     3361 ttgtttttag gtatt
//
LOCUS       DAORKW010000412         3369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1541_length_3369_cov_5.448702, whole genome shotgun sequence.
ACCESSION   DAORKW010000412 DAORKW010000000
VERSION     DAORKW010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1541_length_3369_cov_5.448702"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLB46_13355"
     CDS             <1..389
                     /locus_tag="PLB46_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77558.1"
                     /translation="MSFAVPLQSGAPSKGIFSIDFIDATIGAAVGGHYVSPNDYTGNF
                     TMLVKSDSAWKSPLSTNPNGYRSCIKFINPYLLIACGTTGVDISKNLSWMNISLEGFN
                     VITISPSTNTVLLAGNNGKIGKLKQN"
     gene            complement(397..687)
                     /locus_tag="PLB46_13360"
     CDS             complement(397..687)
                     /locus_tag="PLB46_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77559.1"
                     /translation="MTLGALSEKLRKRVTELRHDSRMQAKINAPLITIEIALGLTELA
                     IDKNRPVSPDEEHWFHESWNIINQLERTEWDDIIDLYRSLAFKMGERNWFRN"
     gene            complement(684..2933)
                     /locus_tag="PLB46_13365"
     CDS             complement(684..2933)
                     /locus_tag="PLB46_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF77560.1"
                     /translation="MKNSLFVLFVTLICQLGSAQTAIFSGVITDANTKEPLIGATVFL
                     NNQTGTATDIDGSFKIVTSEGSYTFTASYVGYEKQSATITLTERASLVVDIALKPIDG
                     ILNTIVVTGSKFEKKLGEETVTIDVIKSDLIQNTNDVKLDQTIQRIPGVSVIDGQANI
                     RGGSGYSYGAGSRVLILMDDLPILTGDAGFPSWDFIPMENIQQVEIIKGAASALYGSS
                     ALNGIINVRTAYPTAEPVTKLSLFSGVYNSPADTAQKWWADDFPFFSGANFAHRQKFG
                     KFDLVTGAYVYTQDSYLKEIYQRRARYNINTRYRINNNLAVGLNVNAQVNRSSSFFFW
                     DGIDSGLYVAAPGTTAFNQGFKITVDPFLNWYDEHGNRHKILSRYYGNHNKTVTTEQS
                     NFNDLFYGEYQYQKHLDKYALVVSSGVLASYAMVSAELYGDSTYNTSNEAVYLQLDKK
                     WFDKLNVSGGVRYEFNQIAGLKESRPVFRLGANYQVANYTFIRASWGQGYRFPTIAEK
                     YISTSVTGLSIYPNPDLTSETGWSGELGVKQGVKISNWQGVIDVSGFISEYDNMMEFT
                     FGYYPFPGFMSLNIGDTRIMGGEISFIGEGKIGTVPTTLIAGYTYIDPKFKDFDSIQN
                     ALSSADYNVLKYRFRHTVKFDVESTIKKFRLGATCNYNSFMEAVDAAFVDPIIPAINL
                     YIIEGLQQYRDEHADGDWIIDIRLAYNINSSSEVSLLCNNVLNREYSSRPAMMDAPRN
                     FTLKYSLTL"
     gene            3091..>3369
                     /locus_tag="PLB46_13370"
     CDS             3091..>3369
                     /locus_tag="PLB46_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HRF77561.1"
                     /translation="MKKLLFVLSFAVLITACSRVPITGRKQVHLLPESQMIAMSLTAY
                     SDFLKESEVVGSGRDYTAVKRVSDKLIPAVESVLKAEGYEEMIADYQWE"
BASE COUNT         1059 a          669 c          559 g         1082 t
ORIGIN      
        1 atatgtcctt tgctgttcct ttacaatccg gtgcgccctc caaaggtatt ttttctattg
       61 attttataga tgcaactata ggcgctgcag tgggcggaca ttatgtttct ccaaacgatt
      121 ataccggaaa ctttaccatg ttagtgaaaa gcgacagcgc gtggaaatca ccgcttagta
      181 ctaatccaaa cggttaccgt tcctgtatta aatttataaa cccttatttg ctaatagctt
      241 gcggaacaac aggagttgat atctcaaaaa acttgagttg gatgaatatt agtttggaag
      301 gttttaatgt aatcaccatt tctccatcta caaatactgt tttattagca ggtaacaacg
      361 gaaaaattgg caaactcaaa caaaactgat tgtttattaa tttctaaacc aattgcgttc
      421 tcccattttg aatgcaaggc tgcgatataa atcaataata tcatcccatt cggttctttc
      481 taattggttg ataatattcc aactttcatg aaaccaatgt tcttcatccg gagaaactgg
      541 tctgttttta tcaatcgcta attcggttaa tcccaaagcg atttctattg taattaacgg
      601 tgcatttatt ttagcttgca tacgactatc atggcgcaac tcagttacac gtttcctcaa
      661 tttttcactc agcgctccta acgtcataat gttagcgaat attttaatgt aaagtttctt
      721 ggtgcgtcca tcatagccgg gcgtgaacta tattcccggt ttaatacatt attacataat
      781 aaggatacct cactacttga atttatatta taggcaagtc tgatatcaat tatccaatca
      841 ccatctgcat gttcatcgcg atattgttgc aaaccttcaa taatgtataa attaattgca
      901 ggaattatcg ggtcaacaaa tgcagcatca actgcttcca taaaactatt gtaattacaa
      961 gtagcaccta gacgaaattt cttgatggtg gattcaacat caaatttaac ggtgtggcga
     1021 aaacgatatt ttaatacgtt gtaatcagca ctggacaacg cattttgaat agaatcgaaa
     1081 tctttaaatt tcgggtcgat gtaagtatat cctgctatta aagttgttgg cactgttcca
     1141 atctttcctt caccgataaa tgaaatttcc ccacccataa ttctggtatc gccaatattt
     1201 agcgacataa atccggggaa tggataataa ccaaacgtaa attccatcat gttatcatat
     1261 tcagaaataa atcctgaaac atctatcacg ccttgccagt tactaatttt aacaccttgt
     1321 ttaacgccta attcgccact ccatcctgtt tctgatgtta aatcaggatt aggatatata
     1381 cttaaacctg ttacggaagt agaaatatat ttttcagcaa ttgtaggaaa acgatatccc
     1441 tgtccccaag aagcacgaat aaaagtataa ttcgctacct gataattagc accaagtcta
     1501 aatacaggtc tcgattcttt taaacccgca atttgattaa attcgtaacg cacaccaccg
     1561 cttacattta atttatcaaa ccatttttta tcaagttgta aatacaccgc ttcattgctg
     1621 gtattatagg ttgaatcccc atacaattcg gcacttacca ttgcataact cgccaataca
     1681 cctgaactaa ctactaaggc atatttatct aaatgttttt gatattggta ttcaccataa
     1741 aataaatcgt tgaaattact ttgttcagta gtaacagttt tgttatgatt gccataataa
     1801 cgggataaaa ttttatgtct gttgccatgt tcatcgtacc aatttaaaaa tggatcaacc
     1861 gtaattttaa acccttgatt aaaagctgta gttcccggtg cggctacata taatccggaa
     1921 tcaatgccat cccaaaaaaa gaaacttgac gagcggttta cctgtgcatt tacatttaaa
     1981 ccaactgcta aattattatt gatacgataa cgcgtattaa tattatagcg ggcacgacgc
     2041 tgatatattt cttttaaata actatcctgt gtgtacacat aggctccagt aactaaatca
     2101 aattttccga atttttgacg atgggcaaaa tttgctccgg aaaaaaaagg aaaatcatct
     2161 gcccaccatt tttgtgctgt atcggcaggt gaattatata caccactgaa taatgataat
     2221 ttagttaccg gctctgctgt tggataagca gtgcgaacat taataatacc gttcaacgca
     2281 ctcgatccat ataaagcact ggcagcacct ttaataattt ccacctgctg aatattttcc
     2341 atcggaataa aatcccagga aggaaatcct gcgtcaccgg ttaaaatggg taagtcatcc
     2401 attaaaatta acacgcgact accggcgcca taactatatc ctgaaccacc tctaatattc
     2461 gcctgaccat caataacact cacacccgga attcgttgaa tggtttgatc caattttaca
     2521 tcattggtat tttgtattaa atcagactta atcacatcta ttgtaactgt ctcctctcct
     2581 agtttctttt cgaatttact tcctgttact acaatcgtat ttaaaatacc atcaattggc
     2641 ttaagggcaa tatctacaac caaggaagcg cgttcagtta gtgtaattgt tgcactttgt
     2701 ttttcataac caacataact ggcagtaaat gtataactac cctcgctcgt tacaatttta
     2761 aacgaaccat caatatccgt tgctgttcct gtctgattat taagaaaaac ggttgcccca
     2821 ataaggggtt ctttggtatt tgcatctgta ataacaccgc taaaaattgc tgtttgcgcc
     2881 gaaccaagtt ggcaaataag cgtcacaaaa agcacgaata ggctgttctt cataaatatg
     2941 tgtgattgtg gggggtaaag ttagccaatg taatcagacg gcaaaacgat aaatagtgag
     3001 tttataaaca cctccaaacc actttatttc gattttgagc atttgagtac gatatttgat
     3061 ttatctacct ttgtaaacta atcgactgtt atgaaaaaat tattatttgt gttatccttt
     3121 gctgtgctta ttacagcatg ttcacgtgtg cctattacgg gaagaaaaca ggtacaccta
     3181 ttacctgaaa gtcaaatgat tgctatgagt ttaactgcct acagcgactt tttaaaagaa
     3241 agcgaagtgg tgggttcagg ccgcgattat actgctgtta agcgtgtttc ggacaaatta
     3301 attccggctg tggaatcggt gttaaaagct gaaggttatg aagaaatgat tgccgattac
     3361 caatgggaa
//
LOCUS       DAORKW010000413         3369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1542_length_3369_cov_4.763126, whole genome shotgun sequence.
ACCESSION   DAORKW010000413 DAORKW010000000
VERSION     DAORKW010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1542_length_3369_cov_4.763126"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLB46_13375"
     CDS             <1..350
                     /locus_tag="PLB46_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77562.1"
                     /translation="EIREFSKARKIFYSEKFDFLEEACAVKELLIFFINNKNSNGVFN
                     IIRNIKPLDLDPALANDYIQALIHPHMLQEHLDEIEANLEDVLDKSDRIEVLELLSNL
                     RSQFDDGDEEEID"
     gene            704..1171
                     /locus_tag="PLB46_13380"
     CDS             704..1171
                     /locus_tag="PLB46_13380"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HRF77563.1"
                     /translation="MNNREIIEAWLAPRLDEMGCFLVDIRINPSINKYEIFIDSPTGV
                     TIDQCMVISRYVEFQMDNDPGFPEKYSLDVSSPGMENPFKVQQQFDKNIGKTVEVVLL
                     NGIKKEGMLKKADGKVLHLEVHHPPKKKGMKPEIELETYGFEEIKSVKKKIIF"
     gene            1257..2498
                     /gene="nusA"
                     /locus_tag="PLB46_13385"
     CDS             1257..2498
                     /gene="nusA"
                     /locus_tag="PLB46_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRF77564.1"
                     /translation="MNSVELVDSFSEFKDVKNIDRPTLMKVLEDIFRTLLRKKYENDE
                     NFDIIVNTEKGDLEIWHRREIVEDGAVENPVTQIALAEALKIEPDFSVGEETYENVSI
                     ESFGRRAILAARQALVQRIMELEKDEVYKKYKDREGEIITGEVYQVWKKEILLLDDDG
                     NELILPKNELIHADNYKKGETLRGVVKKVDFRNNNPVVIVSRTDPKFLEKLLEQEVPE
                     VFDGLIVIKKIVRAPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDI
                     INYTNNNQLLIQRALSPAKISSIELDNEKKKAAVYLAQDQVSLAIGKGGLNIKLACQL
                     TGYDIDVFRDTEEEEMDIDLDEFADEIDEWMIDELKSIGCDTARSVLELSADELVRRT
                     DLEEETVKEIIKILKSEFDED"
     gene            2573..>3369
                     /locus_tag="PLB46_13390"
     CDS             2573..>3369
                     /locus_tag="PLB46_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77565.1"
                     /translation="MSEEKTIRLAKAASEFNVAMSHIVDLLTTKGFSVDAKPTTKLSE
                     EMYTLLLKEFGKDKDLKGKADQLSIGIGRKLEKEKADEVVEPVKKTEEPEVKTEIKPE
                     EKPEIKPEVKPEVKPEVKPEGKPEKKPVVEKQEKAKPEKTEEEKPVEEVEVIKARKEK
                     VEDLKVVGKIDLNKKPAKKEEPPIEKAPPAVTKKSFEVKKADKDAPVKETKKAEEIAP
                     EVEEVVEKKVEVEREMIETKKVTLEGPKIIGKIEIKEEKKPEKRPHQ"
BASE COUNT         1223 a          572 c          727 g          847 t
ORIGIN      
        1 atgaaataag agagttttca aaagcgcgaa aaatatttta ttccgaaaaa ttcgattttc
       61 tggaagaagc ttgtgcagta aaagaattgt taatattttt cataaataat aaaaattcaa
      121 atggcgtatt caatattata agaaatatta aacccttaga cttagatcca gcgcttgcaa
      181 atgattatat ccaagcattg attcacccac acatgttgca ggagcactta gatgaaatag
      241 aagcaaacct tgaagatgtt ctggataagt ctgatagaat tgaagttctt gaactgttga
      301 gtaatttaag aagccaattt gacgacggcg atgaagaaga aattgattaa tttataaaac
      361 ctgtaatttc ttgttaaaat ccgattaagg ccatcctcca attgaggcgt accactgatc
      421 gtattagtcg gtttattcca atgaattaca tcactcaaag aacaaacctc ttatgcctct
      481 ccccgaacat catgattttc cgcctaaaca atagtttacc ggtttattac tttttttagt
      541 taccctcttc cagttcagtt aaccattcat ccccaagtgt aagcaaacta acacaggagc
      601 ttcggtttac taccctcccc tttgtaatct caaaaccttt agctatcttt gcaccacttc
      661 ggaagagggg acagtaaaaa gtcccctctt ttgtttctgt atcatgaaca accgggaaat
      721 tatagaggct tggctggcgc cccgactgga tgaaatgggc tgctttttgg tggatatcag
      781 gattaatcca tctatcaata agtatgaaat ttttatcgat agtcccaccg gcgtaactat
      841 tgaccaatgt atggtaatta gccgctacgt tgagtttcag atggataatg accccggatt
      901 tcctgaaaaa tacagcctcg atgtgtcgag cccgggcatg gaaaatccgt ttaaagtgca
      961 gcagcagttc gacaaaaaca tcggtaaaac ggtggaggtt gttttactga atggcattaa
     1021 aaaggaaggg atgctgaaaa aggcagatgg taaagtgtta cacctggaag tacatcaccc
     1081 cccaaagaaa aaaggtatga aaccggaaat tgagctggaa acatacggtt ttgaagagat
     1141 taaaagtgtg aagaaaaaaa tcatttttta gcataaatta ttgataatca atatattata
     1201 acaaacaaac atcggtttaa ctgccttggg tagaaaaacc aacaaaaaca aatactatga
     1261 acagcgtaga actagtagat tccttttcgg agtttaagga cgtcaaaaac atcgaccgtc
     1321 ccacacttat gaaagtgctg gaagacattt tccgcacctt attgcgcaag aagtacgaaa
     1381 acgatgaaaa cttcgacatc attgtaaaca ccgaaaaagg tgaccttgaa atttggcatc
     1441 gccgcgagat tgtggaagac ggggcagtag aaaacccggt aacacaaatt gcattggctg
     1501 aagctttaaa aattgagccc gatttttcgg taggtgaaga aacatatgaa aatgtgagta
     1561 tcgaaagttt cggtcgccgt gccattttag ctgcccgcca ggctttggtg cagcgtatta
     1621 tggaattgga aaaagacgaa gtttataaaa aatataaaga ccgtgaaggt gaaatcatca
     1681 ccggtgaagt atatcaggtt tggaaaaaag aaatcctgtt attggacgat gatggtaatg
     1741 agctgatttt acctaaaaat gaattgattc acgccgataa ttataaaaag ggtgaaacat
     1801 taagaggtgt tgtaaaaaaa gtagatttcc gcaacaataa tcctgtggtt atcgtttcaa
     1861 gaacagaccc taagttcctc gaaaaattat tggaacaaga agtgccagag gtattcgacg
     1921 gacttattgt gattaaaaaa attgttcgcg cacctggcga aagagcaaaa gtggctgttg
     1981 aatcatacga cgaccgtatt gacccggttg gtgcctgcgt aggtatgaaa ggaagccgta
     2041 ttcacggtat tgtgcgtgag ttacgcaatg aaaacatcga tattattaat tacaccaata
     2101 ataaccagtt attaattcaa cgtgcattaa gtcctgcaaa aatttcatct atcgaattag
     2161 ataatgaaaa gaaaaaagca gcggtttatc ttgcacaaga tcaggtttca ttagcaattg
     2221 gtaaaggcgg tttaaatatc aaattggcct gtcagttaac cggatacgat attgacgtat
     2281 tccgcgatac agaagaggaa gaaatggata tcgaccttga cgaatttgca gatgaaattg
     2341 acgaatggat gatagatgaa ctcaagtcga ttggttgcga tactgcgaga agcgtattgg
     2401 aattaagcgc cgatgagtta gttcgtcgta ccgatttaga agaagaaacg gtaaaagaaa
     2461 ttatcaaaat attaaaatca gagttcgacg aagattgatg taaacattaa ttcatatagg
     2521 tttttgatat atgataaaat gttaatccta tctttgtaac ccaagcaact gaatgtcaga
     2581 agaaaaaact ataagattag ctaaggccgc cagcgagttc aacgtggcaa tgtcgcacat
     2641 cgttgatttg ctgactacaa agggtttttc cgtagatgcc aaacctacaa ccaagctctc
     2701 ggaagaaatg tatacccttt tgctgaagga attcggcaag gataaagact tgaaaggcaa
     2761 agccgatcag ttaagcatag gtattggccg caagctcgag aaggagaagg ctgacgaggt
     2821 tgttgaacct gttaaaaaaa cggaagaacc tgaggttaaa acagaaataa aaccggaaga
     2881 aaagccggaa ataaaacctg aagtgaagcc tgaggtaaaa cctgaagtta aacctgaagg
     2941 taaaccggag aaaaaacctg tagttgaaaa acaggaaaaa gctaaaccgg aaaaaacaga
     3001 agaagaaaaa cctgttgagg aagttgaggt aataaaagcg cgtaaagaaa aggttgagga
     3061 tttaaaagtg gtaggtaaaa ttgacctgaa taaaaaacct gccaaaaaag aagaacctcc
     3121 tatagaaaaa gcgcctcctg ctgttaccaa aaaatccttt gaagtaaaaa aagcagataa
     3181 agacgcgccg gttaaggaaa ccaaaaaagc cgaagaaatt gcacctgagg tggaagaggt
     3241 tgtggagaaa aaggtagaag tggagcgcga aatgattgaa accaaaaaag tgacgctcga
     3301 agggccgaaa attattggta aaatcgaaat taaggaggag aaaaaaccgg aaaaacgccc
     3361 tcatcaaca
//
LOCUS       DAORKW010000414         3352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1551_length_3352_cov_4.690931, whole genome shotgun sequence.
ACCESSION   DAORKW010000414 DAORKW010000000
VERSION     DAORKW010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1551_length_3352_cov_4.690931"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2401
                     /locus_tag="PLB46_13395"
     CDS             <1..2401
                     /locus_tag="PLB46_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRF77566.1"
                     /translation="GAEVGEYSSIQDEQSIVELQARDNDGFYYLTGYYLNETSNFEED
                     SFELVQLSDMFTEQFKKSNYTLVADQFSNQNGYPAYTRTSKSNDSYVHTKVIINGPYY
                     FLLAAFTEQASAPEKFLNSFQLSQFSYIKPFEFYIDTALDFTVKTIVPKVDTLLTIDE
                     ISYGNYYPYTYNDEEEKEDISFISKSKVMLFSSEATPENILVRFHKYNKFYAQDDSKK
                     FWASFDSVYANYNNYHISRRKFAEHDSLQELSFFITDTNSTRAIYRKFILKADVLFSL
                     VTTIDTIKGPSLFVDAFYNTFAPGDSIKGRSIFTSTTDMFFEQLNSEDSVLRKQAINS
                     LITVNLKNKDADDMMQFIQSPRFKTLKADDRATFIYQLGELKHPGIVPLLKTIYIAAG
                     DTSMFQLAALRALSRQKSDTALAAFMELLYIETPLAKEGVTLDYLFYHFEDTIELSTK
                     LFPALLDFISFPEYKTGIYSLLATIRSNDNITSKPHSALKKQILAEATAEYKRNMVTI
                     DEDDYSSGYRDYQNKYYFPSLSSMVGGRTFNFYGNYTGPDEFENYTSYGFDYAYSYED
                     YDVSNYFDYDNLYSEADSLNKFPWAHTLQDYYSILLTTFYDDPAVKKYFDKILKSKDE
                     ELIYTTALTLQTTNKPVPDSVWMNLQKSREWRYIIMKKLTEFKKTELIDSSYTNPLSI
                     TEACLYQYEFKDEGDSIQFIERREVDGELGKGYIYFYKSKLDDDKTWHLDCIGLQPLT
                     DGEYTLEPEFIYFGKVILDEQQLQEQITEIIDTYHLIGRTRVSSSLSNDIYGYDYDYD
                     Y"
     gene            2414..3259
                     /locus_tag="PLB46_13400"
     CDS             2414..3259
                     /locus_tag="PLB46_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRF77567.1"
                     /translation="MMKIKYHFCLLPLLFVLCFSFGQTPYPGVQNNCDSLRYNLSWKR
                     ELAIITPAIIVVTTDLFLPPPTLGVEDLAGLDISDIPAFERDIIYFDSAQGRVAEIWS
                     DGINALSFGACAAAIFSSYKEDGQVFKNAVVFAEGVALTYGVTEVLKSAVGRNRPYTY
                     NENYSTAYRLRKDPTSSFPSGHTSSTAYNCFFAASLIDYYYIDDENKWLRVINWGTAA
                     TIPIITGYLRTEAGKHFYTDIAGGYIIGAAIGLGVPMLHKCNNLHISAGNVNDVKVLN
                     VVVNF"
BASE COUNT         1107 a          504 c          662 g         1079 t
ORIGIN      
        1 aggtgcagag gttggtgaat attcgagcat tcaagacgaa caaagtattg tggagttgca
       61 agcaagagat aacgatggct tttactattt aaccggttat tatttaaatg agaccagtaa
      121 ttttgaagaa gacagttttg agttggttca actctcagat atgtttactg aacaattcaa
      181 aaaaagcaat tatacgttgg ttgcagatca atttagcaat caaaatggct atccagcata
      241 taccagaact tcaaaaagta atgattcata tgtacacact aaagtgataa taaatggtcc
      301 ttattatttc ctgcttgctg catttaccga gcaagcttct gcacctgaaa agtttttaaa
      361 tagttttcag ttatcccaat tcagttatat taaacctttt gaattttata tagatacggc
      421 cttagatttt acggtaaaaa ctatagtgcc aaaggtcgat acactactta cgattgacga
      481 aataagttat ggcaactatt atccttatac atataacgac gaggaggaaa aggaggatat
      541 tagttttata tcaaaaagta aagtaatgtt attttcttcg gaagcaaccc cggaaaatat
      601 tttggtgcgt tttcataaat acaataaatt ttatgcccag gatgattcga aaaaattttg
      661 ggcaagtttt gatagtgtat atgcgaatta taacaactat catattagca gacggaaatt
      721 tgcagaacat gacagtctgc aggagttatc attttttatt actgatacca acagcaccag
      781 ggctatttac cgcaagttta ttttaaaggc agacgttttg ttttcattag ttaccaccat
      841 agacacaata aaaggaccgt cgttatttgt tgatgctttt tataacacat ttgcacctgg
      901 tgatagtata aaaggacgca gcatatttac atctacaaca gatatgtttt ttgaacagct
      961 gaacagtgaa gacagcgtat tgcgcaagca ggccataaat tcattgatta cagtgaattt
     1021 aaaaaataaa gatgccgatg acatgatgca atttattcaa tctccacgtt ttaaaacatt
     1081 gaaggcggat gacagggcta cttttattta tcaattaggt gaattaaaac atcccggcat
     1141 agttccactg cttaaaacaa tttatatagc tgcaggcgat acgtccatgt ttcagttggc
     1201 tgctttgcgg gcattatcca gacaaaaatc tgacaccgct ttagccgctt ttatggaatt
     1261 attatacata gaaacaccgt tagctaaaga aggtgtaacg ctagattatt tgttttacca
     1321 ttttgaagat acgatcgaat tgtcaacaaa attatttccg gcattattgg atttcatttc
     1381 tttccctgaa tataaaacag gtatatatag tctattagca acaataagat caaatgataa
     1441 cattacatca aaaccgcata gcgcactcaa aaaacaaatt ctagccgaag caactgctga
     1501 atacaagcga aatatggtta ctatagatga ggacgactat tcatctggtt atcgcgacta
     1561 tcagaataaa tattatttcc cttcattgag tagtatggtt ggaggacgga cctttaattt
     1621 ttatggaaat tatacgggac cggatgaatt tgaaaattat accagttatg gatttgatta
     1681 tgcatatagt tatgaagatt atgacgtttc aaactatttt gattatgaca atttgtatag
     1741 cgaggcggat tccttaaata aatttccatg ggcgcatact ttacaagatt attatagcat
     1801 attattaact acattttatg atgacccggc agtaaaaaaa tattttgata aaattttgaa
     1861 aagtaaagat gaggaattga tatataccac tgctttaacc ttacaaacaa ccaacaaacc
     1921 ggtgcctgat tctgtttgga tgaacttgca aaaaagtagg gaatggcggt atattataat
     1981 gaaaaagttg actgaattca aaaaaactga attgatagac agcagttata ctaacccgct
     2041 aagtatcaca gaggcgtgtt tatatcaata tgaatttaag gatgaagggg atagcattca
     2101 atttatagaa cgcagggaag ttgatggtga attgggaaaa ggctacatct atttttataa
     2161 aagtaaatta gatgatgata aaacctggca cctggattgt attggtttgc agcctttaac
     2221 tgatggcgag tacacacttg aacctgaatt tatttatttc ggaaaagtta ttcttgatga
     2281 gcaacaattg caggaacaaa taactgaaat tattgatact tatcatttaa ttggaaggac
     2341 aagggtgagc tcatcattat cgaatgacat ttatggatat gattacgact atgattatta
     2401 atggtaagat agcatgatga aaataaagta tcacttttgt ttattgccat tactttttgt
     2461 gttatgcttt tcttttggtc agacaccgta tcctggagtc caaaacaatt gtgatagttt
     2521 aagatataat ttatcgtgga aacgtgaatt ggctataatt actcctgcga taatagtagt
     2581 gacaacagat ttatttttac cccctcccac tttaggtgtt gaggatttag ccggattaga
     2641 tatatctgat attcctgcat ttgagcgcga tattatttat tttgatagtg cacagggcag
     2701 ggttgcagaa atttggagcg atggcattaa tgcactttcg tttggggcat gtgcagcagc
     2761 gattttttca tcgtataagg aagatgggca ggtttttaaa aatgccgttg tttttgctga
     2821 aggtgtcgca ctaacatatg gtgttacgga ggttttaaaa agtgcagtcg gcaggaatag
     2881 gccgtatact tataatgaaa attacagtac ggcttatcgt ttgcgtaaag accctacgag
     2941 ttcatttcca agtggacata cttcttctac tgcttacaat tgtttttttg ctgcatcatt
     3001 aattgattat tattatatcg atgatgaaaa taagtggcta agggtaatca attggggtac
     3061 tgcagcaaca attccaataa ttaccggtta tttgcggacg gaagctggaa aacattttta
     3121 tactgatatt gcgggaggat atataattgg cgcagcaatt ggtttgggtg tgccgatgtt
     3181 gcataaatgt aataatctac atattagcgc ggggaatgtt aatgatgtta aggtgttgaa
     3241 tgtggtggtg aatttttaaa tgaaatttat tgtataccat tttatttttt tgaacagccg
     3301 aaaccacgga gggaatgcaa agagaacagc ccgcgaagtg cacagagttg at
//
LOCUS       DAORKW010000415         3348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1557_length_3348_cov_4.041300, whole genome shotgun sequence.
ACCESSION   DAORKW010000415 DAORKW010000000
VERSION     DAORKW010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1557_length_3348_cov_4.041300"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="PLB46_13405"
     CDS             <1..1305
                     /locus_tag="PLB46_13405"
                     /inference="COORDINATES: protein motif:HMM:NF021642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor SrfC family protein"
                     /protein_id="HRF77568.1"
                     /translation="NSQIYKGFNHFQEEKEEILPKDYPTFREDLKKSFLEFEFVKRHF
                     ANPTESWNEAASINKDGTNLIIEKLTIAANNINRARMSKMLTDLNEISLNVLAELLKH
                     FHNEDNDQGLRKAKDIAGDIQFKLATAFSADKIKNYGQLMKEFMLDEGSVLKLFRNIV
                     DAPEYRDVRIMDIYSTYRIEVPVEVGDTAEKYFERLCIRYEKTTDERKEEFRLELQLN
                     QINLEDLIKGNSDLIKNNAQQLAEALLKYWFEYISLNHKQSIQQILAQDASLDNIKDM
                     YQKLFKKLDFVNRIAEKIRRFVDGNNKTDLPYEIIADISTELLNSCINSVGFEYLDES
                     EINDLKLANLQNKLGLELDNYSNPTENSLEELFTKIDNWTDIIQTQPEEMKSLPSYRN
                     YLSWSNRLKVGFVSVCNIPNYDEIANDNLGKIINECKSINYE"
     gene            1308..>3348
                     /locus_tag="PLB46_13410"
     CDS             1308..>3348
                     /locus_tag="PLB46_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRF77569.1"
                     /translation="MATLINYQFLFSTDLGYFKALQIIGGKTIFDRYKLANAMVQKFE
                     EKYRNFLAYPVKDGNYIEFHGIKSKYDTPQILTELQGNNAIKYQNIKQETLAYYNSKI
                     EEFKAIDKNKSEYLSDAIKSIDDRFVFCYDDKVILGAWGMQLRDNVREDINVIRKSSP
                     RKDIGDKDLKEVVDTNPEIFVKLSPFIISFNPGDNGELNGNSSITKDANSYVLDDEIP
                     KVNPKYGYEFVGWNETPNGYLVAGDKEFIAQYQQIPVTLEKVPRWKRFWLWFTSAGCL
                     KWLLWLLLALFLLLLLSWIFRGCNTGSVGGGALSDNDTTWLREDPSRGYDGGIYDPND
                     PYQPVPTPPGYEDVLPPEQGVLPPIDDKPNIIPGNPSIIGNRLNILMENQDKSILDLA
                     KDFKEKYPGEKYKVVYYDDVVKRMQIEIPQEERERLKQEIPSAFAPKYNLFIFDEALF
                     EGFYEPKNPAIADTAKSWHLNAIHALQAWDITRGSENITVAIVDNGFNLKHPALKSKV
                     VQPYNVWKHSEDISPQQVDHGTHVAGIALAISDNEHGMCGIAPNCKFMPIQVANGRNQ
                     MTTTSVLDGILYALYQGADVINVSLGSMFTGLAKSSEVTQKDLINNHFKEEERLWQHI
                     MQIAANHNSTVVIAAGNDNVLAGIDAIQRPELFITVSAVDKNNSIYNKADFSNYGL"
BASE COUNT         1169 a          571 c          647 g          961 t
ORIGIN      
        1 aacagtcaaa tctacaaagg ttttaatcac tttcaggagg aaaaggaaga aatattacca
       61 aaagattatc caacttttag agaagatttg aaaaagtctt ttcttgagtt cgaatttgta
      121 aaacgccact ttgcaaatcc aaccgaatcg tggaatgaag cagcaagtat caacaaagac
      181 ggcacaaact tgataattga gaaattaact attgctgcaa acaacatcaa tcgcgcaagg
      241 atgtcaaaaa tgcttactga cctcaacgaa atatcgctga atgtccttgc tgaattactt
      301 aaacatttcc acaatgaaga taatgaccaa ggtttacgga aagcgaaaga tatagctggc
      361 gacattcaat tcaagttagc aacagctttc tctgctgata aaataaaaaa ttacgggcaa
      421 ctaatgaagg aatttatgct tgacgaaggt tccgtgctta aacttttccg caacatagtt
      481 gatgctcctg aataccgtga tgtgagaatt atggatattt atagtactta ccgaatagaa
      541 gttcctgtgg aagtgggtga caccgctgag aagtattttg aacgcttgtg tatccgttat
      601 gaaaaaacaa ccgacgaacg taaagaggaa tttcgcttgg aattacaatt gaatcaaatt
      661 aatttagaag accttattaa aggcaactcc gatttgatta aaaataatgc acaacaattg
      721 gcagaggcat tactcaaata ttggttcgaa tacatcagct taaaccacaa acagagtatt
      781 caacaaattt tagcacaaga tgcatctttg gataatatta aagatatgta tcaaaaacta
      841 ttcaagaaat tagactttgt taatcgaata gcagaaaaaa tacgtcgctt tgttgatgga
      901 aataataaaa ccgacctgcc atatgaaatt atcgcggata tttctacaga acttttgaat
      961 agttgcataa atagtgttgg ttttgaatat cttgatgaat cagaaataaa cgatttaaag
     1021 ctagcaaatt tacaaaataa attggggtta gaattagaca actacagtaa tccgactgaa
     1081 aattcgcttg aagagctttt caccaagata gataactgga cagacataat acagacgcaa
     1141 cctgaagaaa tgaagtcctt accaagttat cgtaattatc tctcttggag caatcgtctg
     1201 aaagtaggtt ttgtttccgt ttgtaatatt cctaactatg atgaaattgc taatgacaat
     1261 ttaggtaaaa ttattaatga atgtaaaagt ataaattacg aatagttatg gcaacattaa
     1321 taaattatca atttttattt agtacggatt taggttattt caaggcattg caaattattg
     1381 gtggaaaaac tattttcgac cggtataaat tggcaaatgc aatggtccaa aaatttgagg
     1441 aaaaatacag aaattttttg gcatatcctg tgaaggatgg caattatatt gaatttcatg
     1501 gaataaaatc aaaatatgat acgccccaaa ttttgacgga attacaaggc aataatgcca
     1561 tcaaatatca aaatattaaa caagaaacgc ttgcatatta caacagtaaa attgaagaat
     1621 ttaaagccat agacaaaaat aaatcggaat atttatcaga tgcaataaaa tcaattgatg
     1681 accgatttgt gttttgctat gacgataagg ttattctcgg tgcatggggt atgcaattgc
     1741 gggataatgt gcgggaagat attaacgtaa ttcgcaaaag ttcgccacga aaagatatag
     1801 gtgataaaga tttgaaagaa gttgttgata ctaacccaga aatttttgta aaattatccc
     1861 catttattat ttcattcaac ccaggggaca atggcgaact aaacggtaat tcctctatta
     1921 ccaaagatgc aaattcctat gttttagatg acgaaatacc aaaagttaat ccgaaatatg
     1981 gctacgaatt tgtcggttgg aacgaaacac caaatggcta tctagttgca ggagataagg
     2041 aatttattgc acaatatcaa caaatccctg taacacttga aaaagtccct cgttggaaac
     2101 gcttttggtt atggtttact agtgccggtt gtctgaaatg gttgttgtgg ttacttttgg
     2161 cattatttct gttgttgttg ttgagttgga tattccgtgg ttgcaacact ggttcggttg
     2221 gtggcggtgc tttaagtgac aacgatacta cttggttgag ggaagaccct tcacgcggat
     2281 acgatggagg tatttatgac cccaacgacc cataccaacc agtgccaaca ccgccaggct
     2341 acgaagacgt gctaccacct gaacagggtg tgttgccccc aattgatgac aagcccaata
     2401 taatccctgg taatccctct attattggta atcgcttaaa tattttaatg gaaaaccagg
     2461 ataaatccat tttagatttg gccaaagact tcaaagagaa atacccgggc gaaaaatata
     2521 aagtggttta ttatgatgat gttgtaaaga ggatgcaaat tgaaatacca caagaggaac
     2581 gcgaacgatt gaaacaggaa attccaagtg cattcgcacc aaaatataat ctttttattt
     2641 tcgacgaagc actttttgaa ggtttttacg agcccaaaaa ccctgcaatt gccgacactg
     2701 ccaaatcatg gcatctgaat gcgatacacg cactacaagc ttgggacatt acacgtggtt
     2761 ccgaaaatat tacagttgcc attgtggata acggtttcaa tctcaaacac cctgccttga
     2821 aaagtaaggt ggtacagccg tataatgttt ggaaacattc tgaggatatt tcaccacaac
     2881 aagttgacca tggcactcat gttgcaggaa tagcacttgc catttccgat aacgagcatg
     2941 ggatgtgtgg tatcgctcct aattgtaaat ttatgccgat tcaagtggca aatggtcgaa
     3001 atcaaatgac caccacctca gttttggacg gtattcttta cgcactttat caaggtgctg
     3061 atgtaataaa tgtttctctt ggtagtatgt ttaccggact agccaaatca agcgaagtaa
     3121 cgcaaaaaga tttaattaac aatcacttta aagaggaaga acggttgtgg caacatatta
     3181 tgcaaattgc tgcaaatcat aattctactg tagttattgc agccggaaat gacaatgtgt
     3241 tggctggaat tgacgcgatt cagcgccctg aactgtttat tacagtttcg gcagttgata
     3301 aaaacaacag catttacaac aaagccgatt tcagcaatta cggcctat
//
LOCUS       DAORKW010000416         3343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1559_length_3343_cov_5.257299, whole genome shotgun sequence.
ACCESSION   DAORKW010000416 DAORKW010000000
VERSION     DAORKW010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1559_length_3343_cov_5.257299"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1425
                     /locus_tag="PLB46_13415"
     CDS             280..1425
                     /locus_tag="PLB46_13415"
                     /inference="COORDINATES: protein motif:HMM:NF016994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HRF77570.1"
                     /translation="MRLLFTYLFISILSNTDTIAQIPATYADLIPLCHTLGTEKTLVG
                     LGESTHGTAEFTKIRSEIVKQLVTKYNYRVFILEAEFATCEKMNKYLHSGEGNLDSLM
                     LDLRLFPWVHEDFKVILEWMMMYNQLHPDDGIRFYGMDAQYSKLYAQKDTIIKHYPNE
                     GKQLFEIVEGNISPKQKISDIKHLSNSLALASDTIDLRLQYFITCQLNKLSATTFNTD
                     AYRNENMAQFVKWIYKNGDDTTKLIIWAHNGHLKKQGGKPMGQYLSDAYGDKYVCVGL
                     DFKEGTFLAVDYDAVADRKLVSMTLNPIEKTIAQELDFGRKTIELIRCDTLKRNVYIN
                     AIGAIYVKQPEKKAAFYNKLHRDKQFDYLVITKMSTPIRLLPQYLNN"
     gene            1475..2431
                     /locus_tag="PLB46_13420"
     CDS             1475..2431
                     /locus_tag="PLB46_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77571.1"
                     /translation="MKIGIMLICYFYYNLTYSQQATDTTQFRNPENYNLALQHYLNQI
                     AVDTIHQNNYYQAACYYALLKNQELAFPYLKNAIVKGARGEDVITDTDFNLLKEDTTK
                     WNEIEKLLKLQYAQRNPGITKLDLGYELWMMWIEDQRYRTFKKNNKLNEKSITDIELH
                     DAHLSRLNDLIESFGWPKYSEVGSEGGDAVFFIFQHDRAESMQEILPLFIAAAKEGEA
                     DLTKAAMMIDRYLSYTENVQLYGTQAHRTIKPGQDRNDIPLQLYPIADEENLISRRNA
                     IGMGDFLENCNRLGVTYTPINDRTDYVFIEIKKKWIKAGYLL"
     gene            complement(2545..3012)
                     /locus_tag="PLB46_13425"
     CDS             complement(2545..3012)
                     /locus_tag="PLB46_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77572.1"
                     /translation="MKANFFTLIITVGTILLVSGSCEKTIEVDLNAQYLGNYKIEYTY
                     LYPYLDTNGWNYNTVSSIDNTCELTEDENGDLLLTNFKFFDDIPMHTYIENDSICVPY
                     YNTYAGGASAHGYQAFGNVVDDQFILEIYSSYYDWSLMAPIGLNGTAIATKID"
BASE COUNT         1203 a          560 c          583 g          997 t
ORIGIN      
        1 aatgaattta taaagatgaa agtgtcatgc aatatattac ctgattatta tattaaccag
       61 agtattgatg aatatttaca aaaaattgac gtggtgagag attttacgat tgaacttgta
      121 aaaaaggcta aatgaggcaa ccttttaccc ctgattcatg aaaaatcaat ttgttatgta
      181 atttgtattt ttacaaagtt tcactttaaa tactttgttg cttctaattg taaagaaatt
      241 aagtgtttta caatagaggt tatttcaaat tacaaatgca tgcgtttact ttttacatat
      301 ttattcatca gcatcttaag taacactgat acaatagcac aaatacctgc tacgtatgcc
      361 gatttaatac cactttgtca caccctcggc actgaaaaaa cactagtcgg actgggagaa
      421 tcaacacacg gcaccgccga gttcactaaa attcgctctg agatagtaaa acaactcgtt
      481 acgaaatata attaccgtgt ttttatattg gaggccgaat ttgcgacttg tgaaaaaatg
      541 aataaatatt tgcattccgg cgaaggcaat ttagattcct tgatgttgga tctgcgactt
      601 ttcccttggg tccatgagga ttttaaagta atactagaat ggatgatgat gtataatcag
      661 ctgcatcctg atgatggcat ccgattttat ggaatggatg cacaatattc gaaactatat
      721 gctcaaaaag acactatcat aaaacattac cccaatgaag gcaaacaatt gtttgaaatc
      781 gtggaaggta atataagtcc caagcaaaaa attagtgaca tcaaacattt atccaactcc
      841 cttgctttgg caagtgatac tattgatttg aggttgcaat attttatcac ctgccaactc
      901 aataaattat cagctacaac gtttaacaca gatgcatacc gcaatgaaaa tatggcgcaa
      961 tttgtaaaat ggatttataa aaacggtgat gatacaacta aattgattat ctgggcacat
     1021 aacggtcatc tcaaaaaaca aggcggcaaa ccaatgggac aatatttgtc tgatgcttat
     1081 ggcgataaat atgtttgtgt aggtcttgac tttaaagaag ggacattttt agcggtagat
     1141 tatgatgcag tggctgatcg taaattggtg agtatgacct taaatccaat tgaaaaaacg
     1201 attgctcagg aattggattt tggtaggaaa acgattgaac taattcgatg tgatacctta
     1261 aaacgtaatg tttacataaa cgccatcggt gcgatatatg taaagcagcc agaaaaaaag
     1321 gcagcctttt ataataaact ccatcgcgac aaacaatttg attatttagt aattacaaaa
     1381 atgtcgaccc caattaggtt gttaccacaa tatttaaata attaattact taacacccga
     1441 agttttaatg tctccgttaa ttaaaagatt tacaatgaaa ataggtatta tgttaatatg
     1501 ctatttttat tacaatctta catatagtca gcaagctacc gataccacac agtttcggaa
     1561 ccctgaaaat tataatctcg cccttcaaca ttatttaaat caaattgctg ttgatactat
     1621 tcatcaaaat aattactacc aagcagcctg ttattacgct ttgctaaaaa atcaggagtt
     1681 ggcatttcct tatttgaaaa atgcaattgt taaaggtgcc agaggtgaag atgtaattac
     1741 agatacagat tttaatttat taaaagaaga tacgacgaaa tggaatgaaa ttgagaaatt
     1801 attaaaactt caatatgcac aacgtaatcc cggcatcaca aaattagatt tgggatatga
     1861 actatggatg atgtggattg aagaccaacg ttatcggaca tttaaaaaaa ataataaatt
     1921 aaatgaaaaa tccataactg atattgagct tcatgatgct catttatcta gattaaacga
     1981 cctgattgaa tccttcggct ggccaaaata tagcgaagta ggaagcgaag gtggtgatgc
     2041 agtttttttt attttccagc acgacagagc tgaatctatg caggagatat tacccttatt
     2101 catcgctgct gccaaagagg gtgaagcaga cttaacaaaa gccgctatga tgattgaccg
     2161 ttatttgtcc tacacggaaa acgttcaact ttacggcaca caagcgcatc gcacaattaa
     2221 accgggacag gatagaaatg atataccttt acaattatat cccattgcag acgaagaaaa
     2281 tttaattagt agacgaaatg caatagggat gggcgatttt ttagaaaatt gtaataggtt
     2341 gggtgtaacg tatacgccaa taaatgacag aacagattat gtttttattg agatcaaaaa
     2401 aaagtggata aaagcgggct atttattgta aaatcaatat caaacgttgc agtaaaaaga
     2461 tgagcaaatt caattgcatc caagttctcg aaatggtgtt aattgtcagg atgctcaacg
     2521 acaacctgtt attaatgcat gacattaatc aattttagtt gcaatagcag tgccgtttag
     2581 cccaatgggt gccattaaac tccagtcata gtaggagctg taaatttcta aaataaattg
     2641 atcgtctact acattgccaa aggcctgata accatgcgca cttgcccctc cggcataggt
     2701 attgtaatag ggtacacaaa tactatcatt ctcaatataa gtatgcatgg gaatgtcgtc
     2761 aaaaaactta aaattggtta atagtaaatc tccattttca tcttctgtta attcacaagt
     2821 gttgtcaatc gatgaaacag tattataatt ccatccattt gtgtccagat aagggtacag
     2881 gtaggtgtat tcaattttgt agttgcctaa atattgggca tttaaatcca cttcaattgt
     2941 tttttcgcaa ctgccggaaa ccaataaaat agttccaaca gtgataatta gggtaaaaaa
     3001 attggctttc atgacgagta ataattgggt gattattaat ccaaatatat ttattgcacg
     3061 gcaaataagc aagttagtat aaaaaaaatg acaattcaaa aacaatttag gggcagttta
     3121 ttcaacaact aaatgctaca aacagacaac cacaaaaagt atgattaatt tatcagccaa
     3181 tgaacaaaat tgacggcaat gataatgtta tacaatacat cccattcgat gctattattc
     3241 caatgaaccc acaatccaca aataaaaaca accccaaaat cgcaaactcc ggcctcattg
     3301 ttttacgcta gtgcagctta cgacacgaaa gcaacgaagc gaa
//
LOCUS       DAORKW010000417         3335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1565_length_3335_cov_4.962500, whole genome shotgun sequence.
ACCESSION   DAORKW010000417 DAORKW010000000
VERSION     DAORKW010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1565_length_3335_cov_4.962500"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLB46_13430"
     CDS             complement(<1..243)
                     /locus_tag="PLB46_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF77573.1"
                     /translation="MAKSIPPKPITTFQEYFDAAPAHGKAHLVALNNIILSVVPDAEP
                     YFSYGIPSYKKNGGLVGMGVFKDHISLFMLNGSFLSN"
     gene            complement(243..716)
                     /locus_tag="PLB46_13435"
     CDS             complement(243..716)
                     /locus_tag="PLB46_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77574.1"
                     /translation="MKKWFVNTEFVLLFLVIVAMILKQLHLNGSDLLLLVSLLSLANL
                     YLFFGITSNLPITEKASPYLTDKTLSKPESITLLVSGISISIVLIGVLFEVLKWGGGA
                     NMLMIGLTSGLLTLIIQYFIVRILHAEIFKFIFYRQLSIMAMSAALWYLGSKTIM"
     gene            complement(720..1088)
                     /locus_tag="PLB46_13440"
     CDS             complement(720..1088)
                     /locus_tag="PLB46_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRF77575.1"
                     /translation="MKPFYTILFLIISNVFMTLAWYGHLQFKKMGWFNGSGLWVIILI
                     SWGLAFFEYCFQVPANRIGSSTYGGPYSMFQLKIIQEVITLVVFTLMAVFVFKTDTLK
                     WNYLAGFFCMVLAVYFIFKK"
     gene            complement(1132..2094)
                     /locus_tag="PLB46_13445"
     CDS             complement(1132..2094)
                     /locus_tag="PLB46_13445"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF77576.1"
                     /translation="MRFRLIAILLLCSKLAFTQSTVTYYFNSSYKLVSNPKNATYRAE
                     VTTLTKIFEGQADINVYTISDTLIQRYSYSDVKKGTRSGSLLLFYETGEPKRMIVYNT
                     EGQPIEDKQFLQDGQIDVITKYQGRVITEYLHFYYPNKQLHNVTKFVSGGLIDSSITY
                     YQSGGKRSIITYFPGKTVKTKILYNEDGTVLSQVIYNDKGVVTSELNSNAANAHPDDF
                     DILKQAIEYKGGSATLYQYIGAEIQYPLAAKDNAITGTTYIKFFVSPTGEVSNASIFL
                     PTFPLLDFEALRVVYNMPSYVIGKQENAGNGSWYRLPVKFALQQ"
     gene            complement(2134..>3335)
                     /locus_tag="PLB46_13450"
     CDS             complement(2134..>3335)
                     /locus_tag="PLB46_13450"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRF77577.1"
                     /translation="QRELLDMEVAYREVAEKLNFHSNQYNQENIKYHQQLNKFQSVEQ
                     DLNYKRSRRDEAIATVNSSKKQLEEVVQEITDTNVKLEEIREVLLKMYDEKTVMEQML
                     GREEETYYGSRGEVAETEEKIKALQKKKDSSDHALGSIKDSLNDMKVQLAGIKERLWV
                     EFQVELDSIIDNAPPQDITITELQEKVDAAKKRIENYGEVNPMAIQAFEEMKTRYDFI
                     KTQREDLLSAKASLLTTIQEIDETARKKFDDAFVQIRDNFKMVFRSLFNEEDDCDLIL
                     KDDYDPLEAQIEIIAKPKGKKPQVIDQLSGGEKTLTGMSLLFALYLIKPAPFCILDEV
                     DAPLDDNNIMKFNNTIRKFADKSQFIIVTHNKRTMAEVDVIYGVTMPEQGISKVVPVD
                     FRGLSQN"
BASE COUNT         1057 a          645 c          525 g         1108 t
ORIGIN      
        1 gttagataaa aaactaccat ttaacataaa taaactgata tggtctttaa acacacccat
       61 tcccactaat ccaccatttt ttttataaga tggaatacca taactaaaat agggttctgc
      121 atccgggaca acggacagaa taatattatt taatgctaca agatgtgctt taccatgtgc
      181 aggtgcagca tcgaaatatt cctgaaaagt ggtaattggt tttggtggaa ttgattttgc
      241 cattacataa ttgtttttga acctaaatac cataatgccg ccgacatagc cataatggaa
      301 agctggcggt aaaaaataaa tttaaaaatc tcagcatgta atatccttac aataaaatac
      361 tgaataataa gtgttaaaag accggatgtg agtcctatca tcagcatgtt tgcaccgcct
      421 ccccatttta aaacttcaaa taaaacacca atcaacacta tacttatact gataccggaa
      481 acaagtagcg tgatggattc tggtttggat aatgttttat ccgttaaata tggtgaagct
      541 ttttcggtta tagggagatt tgaggtaata ccgaaaaaca aatataaatt agccagactt
      601 aataagctga ccaataataa taaatcagag ccatttaaat gaagttgttt taatatcatg
      661 gcaacaatta ccaggaacaa cagcacaaat tcagtattta caaaccactt tttcatacat
      721 cattttttaa agataaaata cacagctaat accatacaaa aaaatccggc cagataattc
      781 cattttaaag tgtcggtttt aaaaacaaaa acagccataa gggtaaacac caccagcgta
      841 atcacttcct ggattatttt taattgaaac atgctgtaag gcccgccata ggttgaactg
      901 cctatacgat tagcaggcac ttgaaaacaa tattcaaaaa atgctaaccc ccaactgatt
      961 aatataatta cccataaccc agaaccattg aaccacccca tttttttaaa ttgtaaatgt
     1021 ccataccagg caagtgtcat aaacacattc gaaatgatta aaaataaaat ggtataaaat
     1081 ggtttcatac agaagcgact gaaaatttaa ttgcgaaata aaaataccat tttattgctg
     1141 caatgcaaat tttaccggga gacgatacca actgccatta cctgcatttt cctgtttgcc
     1201 tattacatac gaaggcatgt tgtagactac tcttaatgcc tcaaaatcga gtaggggaaa
     1261 ggtgggtaaa aaaatactgg cattacttac ttctcctgta ggagacacaa aaaatttaat
     1321 atatgttgtg cctgtaatag cattgtcttt agctgcaagt ggatattgaa tttcggcacc
     1381 aatatattgg tacaatgtag cacttccacc tttatattca atagcttgtt ttaaaatatc
     1441 aaagtcgtca ggatgtgcat ttgcagcgtt gctattgagt tcagaagtta caacaccttt
     1501 atcattatat atcacctgac ttaacacagt gccatcttca ttatataaaa tttttgtttt
     1561 tacggtttta cccggaaaat aagtgatgat ggacctttta cctccacttt gataataagt
     1621 aattgaagaa tcaattaacc ctccgctaac aaatttggtt acattatgca gttgtttgtt
     1681 tgggtaataa aaatgcagat attctgttat tacccgtccc tgatattttg taataacatc
     1741 tatttggcca tcctgcaaaa actgtttatc ctcaattggc tgaccttcgg tattataaac
     1801 aatcatccgt ttgggttcac ctgtttcata aaataaaagc agcgaaccac tgcgcgtacc
     1861 cttttttaca tcactataac tatatcgttg aatgagggta tcggaaatgg tataaacatt
     1921 aatatctgcc tgaccttcaa aaattttggt cagcgtagta acctcggccc ggtaagtagc
     1981 atttttaggg ttactcacta acttataact actattaaaa taataggtaa cggttgactg
     2041 tgtaaatgcc agcttggaac ataacaataa tatagcgatg agcctgaaac gcataagatt
     2101 gttaaagttg agaaatttta actaaaattc caatcaattt tgacttaagc cgcgaaaatc
     2161 aaccggcaca accttactta taccttgttc aggcatggta acaccataaa ttacgtcaac
     2221 ttcagccatt gtgcgtttgt tgtgcgtcac aataataaat tgtgatttat ctgcaaactt
     2281 tctgatggtg ttattaaact tcataatgtt gttatcatcc aatggtgcat ccacctcatc
     2341 gagaatacag aatggagccg gtttaatgag gtagagtgca aacaataaac tcataccggt
     2401 taatgttttt tctccaccac tcaactggtc aattacctgt ggttttttac cttttggttt
     2461 agctatgatt tcaatttgtg cttcgagcgg atcgtagtcg tcttttaaaa ttaaatcgca
     2521 atcatcttct tcattaaata atgagcggaa taccatttta aaattatcgc gaatttgcac
     2581 aaatgcatca tcgaattttt ttcttgccgt ttcatctatt tcctgaattg tagtaagtaa
     2641 cgatgctttt gcgcttaata aatcctcacg ttgtgtttta ataaaatcat aacgtgtttt
     2701 catttcttca aatgcctgaa tagccatagg gtttacttcc ccataatttt ctatccgttt
     2761 tttagcagca tccacttttt cctgtaattc agtaatggtg atatcctgcg gaggtgcatt
     2821 atcaataata ctgtctaatt caacctggaa ttccacccac aaacgttctt taattccggc
     2881 taactgcacc ttcatatcgt tgaggctgtc tttaattgag cctaatgcat gatcactgct
     2941 gtctttcttt ttctgtaatg ctttaatttt ttcttctgtt tcagctactt ctccgcgact
     3001 tccataatac gtttcttcct cacgaccgag catttgttcc atcacggtct tttcatcgta
     3061 catttttaat aatacttcgc gtatttcttc cagtttaaca ttggtatcgg ttatttcctg
     3121 aacaacttcc tctaattgtt tcttgctgga attaacagtc gcaattgcct catctctgcg
     3181 tgagcgtttg taatttaaat cctgttcaac actctggaat ttattgagct gttgatgata
     3241 tttaatgttt tcctgattgt actgattgct atggaaattt aatttttcag ctacttcgcg
     3301 ataagcaact tccatatcca acaattcgcg ctgac
//
LOCUS       DAORKW010000418         3329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1569_length_3329_cov_5.667379, whole genome shotgun sequence.
ACCESSION   DAORKW010000418 DAORKW010000000
VERSION     DAORKW010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1569_length_3329_cov_5.667379"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1270)
                     /gene="hflX"
                     /locus_tag="PLB46_13455"
     CDS             complement(86..1270)
                     /gene="hflX"
                     /locus_tag="PLB46_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRF77578.1"
                     /translation="MAEVIQTNKKERAVLIGLVTKDQTLEQLNEYLAELEFLTTTAGA
                     ETISTFYQRLPHPDHRTYVGKGKLQEINQFVQDNAIDLVVIDDEISPSQLRNIEAELK
                     IKIVDRSMLILDIFATRAQTVQAKTQVELAQTQYMLPRLKGLWSHLDRIKGGIGMRGS
                     GEKEIETDRRVAQQKIALLKEKLLKIGLQNETQRKNRGELIRVALVGYTNVGKSTLMK
                     VLSKSEVFAENKLFATLDTTVRKVVLDRTPFLLSDTVGFIRKLPHHLIESFKSTLDEV
                     RESDILLHVVDIAHPAFEEHINVVNDTLKELEAQEKPTILIFNKMDLYEERYFDKYLT
                     NEIKKDILDELKNSWMQKSNAHCVFISATEKNNIEELRNRLTDLVKEQYQIRYPYKTE
                     FF"
     gene            1473..2360
                     /locus_tag="PLB46_13460"
     CDS             1473..2360
                     /locus_tag="PLB46_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77579.1"
                     /translation="MNIQYLRHKDIDFEKWDNCIDHAINALPYAYSWYLNIAAEEQWD
                     ALVIDDYKAVFPLPFKNRVVFKQIYQPFFVQQLGLFYTDVALANHLHYCVEQIPSAFR
                     KMQLQLNTYNEMSSSFVKVKQKITHHIDLHKPYETLLSAYAVNNKRNVKKAMKANLNT
                     VTLNTANELIAFRKKYLAEELAGVQNDNDLERLRKLLEKALSLNKGFIKGVVDEHNQL
                     LALCFILKSNKMLIYLSAVSSEKGKELNAMSLLIDGIIQQYANTDFIFDFEGSMIPGL
                     ARYYKGFGGVEKIFPVIQK"
     gene            complement(2364..3068)
                     /locus_tag="PLB46_13465"
     CDS             complement(2364..3068)
                     /locus_tag="PLB46_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRF77580.1"
                     /translation="MEHFPLNVIHDISNIIYPKLCAACHETLNKHEKVICLKCQLGLP
                     KTDHVLYDDNPVAKKFWGKVPIERALAMYHFHKTSRLQEVMHALKYKGRKDVGLRLGN
                     LMGYHLQQYHVFSEIDYLTAVPLHKDKLAKRGYNQSGIIAEGLSEVLQIPYDADILQR
                     SLFTETQTKKSRMERWENVKDIFEVQKQELVTNKHILLLDDVITTGSTLEACAVKLIA
                     NQGTKVSIVAVAHAEL"
BASE COUNT         1013 a          641 c          554 g         1121 t
ORIGIN      
        1 gaactagctt aaatccgttc ctagtattaa aatttacttt aaaaatagaa ttttaattta
       61 gcattcccgt ggttccggct gttcttcaga aaaattcagt tttataagga tatcgaattt
      121 ggtattgctc ttttaccaaa tcagtgaggc gattgcgcaa ttcttcaata ttattttttt
      181 cggttgcaga aataaacaca caatgcgcat tcgatttttg catccacgaa ttttttaatt
      241 catctaaaat atcttttttg atttcgttgg tcagatattt atcaaaatat ctttcctcat
      301 aaaggtccat tttattaaaa attaaaatag tcggtttttc ctgcgcttcc aactctttta
      361 aagtatcatt tactacatta atatgttcct caaatgctgg atgtgcaata tcaactacat
      421 gtaataatat atcactttca cgcacctcat ccaatgttga tttaaaactt tcaattaaat
      481 gatgcggtaa tttgcgaata aacccaaccg tatcacttaa caaaaaaggt gtgcgatcca
      541 acaccacttt tcgaactgta gtatccagtg ttgcaaataa tttattttcc gcaaacactt
      601 cacttttact caacaccttc atcagtgtcg atttgcccac attggtataa cccaccaagg
      661 caacacgaat taattcccca cgatttttgc gctgcgtttc gttttgtaag ccaattttta
      721 acagcttttc ttttaacaac gcaatttttt gttgcgcaac acgtcggtca gtttcaattt
      781 ctttttcacc cgaaccacgc ataccaatac cacctttaat cctgtccaaa tgcgaccaca
      841 acccttttaa acgtggaagc atatattgcg tttgcgctaa ctccacctgt gtttttgcct
      901 gcaccgtttg cgcacgtgtt gcaaaaatgt cgagaatcag catactgcgg tcaacaattt
      961 taatcttaag ctccgcttca atattgcgca gttgtgatgg agaaatttca tcgtcaatca
     1021 ccactaaatc tatagcatta tcttgcacaa attgatttat ttcctgcaac tttcccttcc
     1081 cgacataagt acgatggtcg gggtgaggca ggcgctggta aaaggtactt atcgtttctg
     1141 ctcctgcagt ggtggttaaa aactctaact cagccagata ctcatttaac tgctccaggg
     1201 tttggtcttt ggtaaccaac ccaataagca ctgcacgttc ttttttattt gtttgtatta
     1261 cttctgccat gtattgcaaa ggtagttcaa tttgacggct ctgtagcaga aagcattgtg
     1321 gtgatgctga tatacataaa atgcactcca aaccaccagc gcagagagtg gacattggtt
     1381 cttttagccc tgaaatctta aattacctaa atgttacgtt aattattggc atatttctga
     1441 attattgctt agcatcgcac catcattata taatgaatat ccaatacctc cgtcataagg
     1501 acatcgattt tgaaaaatgg gataactgta tcgaccatgc cattaatgca ttgccgtatg
     1561 cttattcctg gtatttaaat attgctgcgg aggaacaatg ggatgcattg gtaatcgacg
     1621 actataaagc tgtttttccg cttccattta aaaacagggt tgtcttcaaa caaatttatc
     1681 agccattttt tgtccagcaa ttgggtttgt tttataccga tgttgctttg gctaatcatt
     1741 tgcattattg tgttgaacaa attccttcgg cgtttagaaa aatgcagttg cagctcaata
     1801 catataatga aatgtccagc tcatttgtta aggtgaaaca gaaaattacc catcatattg
     1861 atttacacaa accttacgaa acactacttt cagcttatgc agtaaataat aaacgcaatg
     1921 taaaaaaggc aatgaaggct aacctgaaca cggttacttt gaatacagcc aacgaattaa
     1981 ttgcatttcg aaaaaaatat ttggcagaag aactggctgg tgttcaaaac gacaatgatt
     2041 tagagcgttt gcgtaaattg ctcgaaaaag cgttgagttt aaacaaagga tttattaaag
     2101 gtgttgttga tgagcataat caactgttgg cattatgttt tatcctgaaa agtaataaaa
     2161 tgttaattta tttatctgcg gtgtcgtcgg aaaaggggaa agaattaaat gctatgagtt
     2221 tattaatcga tggcataatt cagcaatatg ccaatactga ttttatcttc gattttgaag
     2281 gttctatgat tcccggactt gcccgttatt ataaaggatt tggcggagta gaaaaaattt
     2341 tccctgttat acaaaagtaa ccactacaat tctgcatgtg caactgcaac tatactaact
     2401 tttgtgccct gattggcgat tagtttaact gcacaagcct ctaatgtaga accggtagtg
     2461 attacgtcat ccaataataa aatatgttta ttggtcacta attcctgttt ttgcacttca
     2521 aaaatatcct tcacgttttc ccaacgttcc attcgcgatt tttttgtttg tgtttcagta
     2581 aacagactgc gttgtaaaat atccgcatca tatggtattt gtaatacctc acttaatcct
     2641 tctgcaatta ttccgctttg attgtaacca cgtttcgcaa gtttgtcttt atgtaaaggc
     2701 actgcagtta aataatctat ttcactaaat acatgatatt gttgtaaatg atatcccatt
     2761 aaattgccca gccgcaaacc gacatctttt ctgcctttgt attttaatgc atgcattact
     2821 tcctgtaaac gggacgtttt atgaaaatga tacatcgcta atgcgcgttc aataggcacc
     2881 ttaccccaga attttttggc aaccggatta tcatcatata aaacgtggtc ggttttcgga
     2941 agccctagct gacattttaa acaaattact ttttcatgtt tatttaatgt ttcgtgacaa
     3001 gcggcacaaa gtttgggata tatgatatta ctaatatcat gtatcacatt taagggaaaa
     3061 tgttccatca attattattt aagggtaaac aatatttaca aagtgaagtc aataatgaca
     3121 taattgcccg gccattaatt accaagttaa ttgagtgcca aagcaatcgc taaaccattt
     3181 aactaattcc gaattgaagc ggctaagttg ttacttgtta ggggtttaaa cgtgtaagca
     3241 cgtttacaaa taataaaagg aaggaatgtt acactccggt aaccacattt caatctgcac
     3301 atacaacatc gttttaaagg taaataatg
//
LOCUS       DAORKW010000419         3324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1578_length_3324_cov_5.187519, whole genome shotgun sequence.
ACCESSION   DAORKW010000419 DAORKW010000000
VERSION     DAORKW010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1578_length_3324_cov_5.187519"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLB46_13470"
     CDS             complement(<1..252)
                     /locus_tag="PLB46_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77581.1"
                     /translation="MKNIFTIFSKEIKDILRDKRTLTVMLLIPLVVYPAIFIGITKYM
                     TAQNEKAVAQTLRVGIINNGNQSDFETFVSTMPNTKISAV"
     gene            complement(273..1055)
                     /locus_tag="PLB46_13475"
     CDS             complement(273..1055)
                     /locus_tag="PLB46_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF77582.1"
                     /translation="MITITNLSKSFQLSRQQKKEMGNAQHGDTVHAVKNISLTCQPGR
                     VFTLLGPNGAGKTTTLRMIATILKPTSGTIVVNGYDTVANGKEVRQNLGFLTGSTGLY
                     DRLTPDETINYFGRLNGMDETTLQRRKKELFDLLGIHEFAHRRVGKFSTGMKQKVSIA
                     RTMIHDPQVVVFDEPTSGLDVIAAKSIIELIRQCKRDNKTVILSTHIMSEVNMLADDL
                     AIIYRGDLIYNGLFTDFKQGMREATLEDEFIRMAQEHEPATI"
     gene            complement(1063..1926)
                     /locus_tag="PLB46_13480"
     CDS             complement(1063..1926)
                     /locus_tag="PLB46_13480"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRF77583.1"
                     /translation="MFKIIAVYIIGICLHVGTLAAQEFNNSLLWKISGNGLTTPSYIF
                     GTIHMIDQKDFVVQPEVDSVFNLAGKACFELKMDDISMLAVYNKWLTLPEGKTLKDYC
                     TDDEFIQLKQYMQDSLQTDIQTIINQKPFVIYQMQSTNFIKGEMASFELYFVQNCMQK
                     GKPIGGLEKLETQLAVFDAIPYEEQIDWVVDGINDSDSSYRYYDTLIHYYLTADLVSL
                     SNFMKESDEEFKKYGPLMLDNRNINWIPVIEEQIKIQSTFIAVGAGHLVGEKGILQLL
                     HNQGYVISPIQ"
     gene            complement(1938..>3324)
                     /locus_tag="PLB46_13485"
     CDS             complement(1938..>3324)
                     /locus_tag="PLB46_13485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRF77584.1"
                     /translation="LTMVAENPGNITGWLADHNGVLRIAISTDGVNSSLLYRNTADEN
                     FEKIVTTNFKEGISPLFFDFQNVELYALSNIGRDKIAIVKYNPILKAETELLFEHPEV
                     DIDNLGYSKKRKVITTAAYTAVKREFKFFDEWSARRYQKVISLIEEETEVYITDADKA
                     EEKFIVRTTSDTSLGNYYLYDEPSHTLTHLADVSPWLKSDMLCAMQPITYTSRDGLVI
                     HGYLTLPQNKQPQNLPLVVNPHGGPWARDTWRFNPEVQFLANRGYAVLQMNYRGSTGY
                     GKQFWEASFKQWGKKMQDDITDGVLHLIQLGIADKNKVAIYGASYGGYAALAGLTFTP
                     ELYACGVDYVGVSNLFTFMETIPPYWEPYIAMMYEMVGHPEIDAALLAASSPVMHIDK
                     IKAPLFVAQGAMDPRVKKSESDQIVTALREKNIDVDYMVKDNEGHGFANQENRFEFYT
                     AMEQFLAKHMQ"
BASE COUNT          990 a          619 c          566 g         1149 t
ORIGIN      
        1 aactgcactt attttagtgt tgggcattgt gctgacaaat gtttcaaaat cagattggtt
       61 gccgttattt ataataccaa cacgcaaggt ttgagctacc gctttttcat tttgtgctgt
      121 catatattta gtaataccaa taaaaattgc aggataaaca accaatggaa taagtaacat
      181 tactgttagt gtgcgtttat cgcggagaat atcttttatt tcttttgaaa agatggtgaa
      241 tatatttttc ataataacga ttagaaagtg aattaaatag ttgccggttc gtgttcctga
      301 gccatacgaa taaattcgtc ttcaagtgtt gcttcccgca tgccttgttt aaaatcggtg
      361 aataagccgt tatagattaa atcaccgcga taaataatgg ctaaatcatc ggcaagcata
      421 tttacttcac tcataatatg ggtagaaaga ataacagttt tattgtcgcg tttacactgt
      481 ctgattaatt cgataatact ttttgcggca attacatcta aacctgatgt aggttcatca
      541 aacactacta cttgagggtc gtgaatcatt gtgcgcgcta tcgatacttt ttgtttcata
      601 ccggtactaa atttccctac acgacgatgc gcaaactcat gaatacccaa taaatcgaat
      661 aattcttttt ttctacgttg taatgttgtt tcatccatgc catttagtct gccaaaatag
      721 ttgatagttt catccggtgt taagcggtcg tataaacctg ttgagcctgt taaaaaacct
      781 agattttgac gtacttcttt tccattggct acagtgtcgt aaccatttac cactatagta
      841 ccggaggtag gttttaatat ggtggcaatc atgcgcaatg tggttgtttt accggcacca
      901 tttggtccta acaaggtaaa tactctgccg ggttgacaag tcaacgaaat gtttttaaca
      961 gcatgtacgg tgtcgccatg ttgtgcattg cccatttctt ttttttgctg tctggatagc
     1021 tgaaacgatt tggataaatt ggtaattgtt atcataattc atttattgta taggtgaaat
     1081 tacatatccc tggttgtgta ataattgtaa aatgcctttt tcgccaacaa gatgtccggc
     1141 accaactgct ataaatgtgg attgaatttt aatttgttct tcaattacag gaatccaatt
     1201 tatatttcgg ttgtcgagca tgagtggtcc gtatttttta aattcttcat cgctttcttt
     1261 cataaaattt gataagctaa cgaggtcggc agttaaataa taatggatga gtgtatcgta
     1321 atatcggtaa ctggaatcag aatcattaat accgtctaca acccagtcta tttgctcttc
     1381 gtagggtatt gcatcaaaaa cagccaattg agtctccaat ttttctaatc cgccaattgg
     1441 tttacctttt tgcatacagt tttgaacaaa atataattca aaagatgcca tttcaccttt
     1501 aatgaaattg gtggattgca tttgataaat gacaaatggt ttttgattaa ttatggtttg
     1561 tatgtcagtt tgcagactat cctgcatata ttgttttaat tgaataaatt catcgtcagt
     1621 gcaataatct tttagtgttt taccttcggg cagggtcaac catttattgt atacagccaa
     1681 catactgata tcgtccattt ttaattcaaa acaagctttg cctgccaaat taaaaacgga
     1741 atctacttca ggttgaacaa caaaatcttt ttgatctatc atatgtatag tgccaaaaat
     1801 ataggaaggt gttgttaaac cattgccgct aattttccat aataaggagt tgttaaattc
     1861 ttgtgcagca agtgttccaa catgtaggca aattccaata atataaactg cgattatttt
     1921 gaacatagtt tgattattta ttgcatatgt ttagctaaaa attgttccat agcggtataa
     1981 aattcaaaac gattctcctg attcgcaaaa ccgtgccctt cgttgtcttt taccatataa
     2041 tctacatcaa tatttttttc acgtaatgct gtaacaattt ggtcgctttc cgattttttt
     2101 actcgtgggt ccattgcgcc ctgagccaca aacaacgggg ctttgatttt atctatatgc
     2161 attacaggag aagaagcagc caaaagtgct gcatcaattt ccggatgacc aaccatttca
     2221 tacatcatgg caatataagg ctcccaataa ggaggaatag tttccataaa tgtgaataga
     2281 ttacttactc ctacatagtc cactccacac gcatataatt cgggtgtaaa tgttaatcct
     2341 gccaatgcgg catatccgcc ataactagcg ccataaattg ctactttgtt tttatcggct
     2401 atccctaatt gaattaaatg caacacccca tccgtaatat catcctgcat ttttttgccc
     2461 cattgtttaa atgatgcttc ccaaaattgt tttccataac ctgttgaacc tctataattc
     2521 atttgcaaaa cagcatatcc tctgttcgct aaaaattgca cttcgggatt aaatcgccag
     2581 gtatctcttg cccatggacc accatgcgga ttaacaacta atggtaaatt ttgtggctgc
     2641 ttattttgtg gcagcgttaa atatccatga atgaccaaac catcacgtga tgtataagta
     2701 attggctgca tggcacacaa catatcactt tttaaccagg gacttacatc ggctaaatgc
     2761 gttaatgtat gcgaaggttc atcgtataaa taataattac ctaaagaagt atcgctggta
     2821 gttcttacta taaatttttc ttctgctttg tccgcatcag ttatataaac ttcggtttct
     2881 tcttcaataa gtgaaattac cttttggtag cgtcgtgcgg accattcatc aaaaaatttg
     2941 aattcgcgtt taacagcagt ataagcagct gttgtaatta cttttcgttt tttggagtac
     3001 cctaaattat cgatatctac ttcaggatgt tcaaataata attctgtttc agctttaagt
     3061 atagggttgt attttacaat ggcaatttta tcgcggccaa tattacttaa tgcatataat
     3121 tccacatttt ggaaatcgaa aaaaagcgga gaaatacctt ctttaaaatt agtagtaaca
     3181 attttttcaa aattttcatc cgcagtattg cgatataata aactcgaatt tacaccatca
     3241 gttgaaatag caatccgcaa aacgccattg tggtctgcca gccaaccggt aatatttccc
     3301 ggattttctg ctaccatagt caac
//
LOCUS       DAORKW010000420         3322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1581_length_3322_cov_3.971534, whole genome shotgun sequence.
ACCESSION   DAORKW010000420 DAORKW010000000
VERSION     DAORKW010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1581_length_3322_cov_3.971534"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLB46_13490"
     CDS             complement(<1..288)
                     /locus_tag="PLB46_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HRF77585.1"
                     /translation="MSQNKQPWQTLSKKIIYDNPWIHVEEHDVINPGGHAAIYGVVQF
                     KNYALGVIPIDNEGNVYLIGQHRYPFNEYTWEIPEGGGNKNEDPLVNIKREL"
     gene            complement(291..971)
                     /locus_tag="PLB46_13495"
     CDS             complement(291..971)
                     /locus_tag="PLB46_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRF77586.1"
                     /translation="MRIVVLTGAGISAESGIPTFRGAGGLWEGHRIEDVASPEGWNKN
                     PELVLDFYNQRRKGIKNALPNSAHIALAELEKQHRVDIITQNIDDLHERAGSKNILHL
                     HGEILKARSIKNPNLITPINGDIQIGDVAADGAQLRPHIVWFGEQVPMLEMAIPLVEQ
                     AEILVIIGTSMVVYPAAGLLHYASPEIPVYVINPEIPEMSRSKNIHFIQKNAGEGVTE
                     FIRLVLTK"
     gene            complement(1502..2983)
                     /locus_tag="PLB46_13500"
     CDS             complement(1502..2983)
                     /locus_tag="PLB46_13500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRF77587.1"
                     /translation="MIIPDAHQIKQLDAATIVAQNIRSSDLMERAAKGITNYLLHDNP
                     HFEFFIINCGTGNNGGDGLCIARQLLQAGKKVKVNICGNVQKSSADFALNFQRLQTQF
                     PLVIHIISSAETIPSFDCDCLLDCLFGTGLNKSITGLPALIIEKMNAADAIILAVDIP
                     SGLQSNSASVEPIVKADCTISFEYPKLAFLFPENAKYVGQWMIQNIGLIDTQRAGLHQ
                     HLHFVTADDIQSLFSERPVFAHKGSFGHVLTIGGSVGMEGAATMSGLAALKTGAGLST
                     ITSSQLQNEHPEIMFTAFENINSTITNKKINAVSIGPGLGNSSSTFQILSELFQAYRQ
                     PIVVDADALNCMAEHPELLNNIPTNTILTPHPKEFERLFGTYQQWFELPDKMIQLAQK
                     YQVIILYKRAYTIIACPDGNVYFNSTGNAGMATAGSGDVLTGIISGLLAQSFAPKEAA
                     IAGVYLHGLAGDLALENNNNSNLIATDIIHQIPLAKAIIMGTE"
     gene            complement(2980..>3322)
                     /locus_tag="PLB46_13505"
     CDS             complement(2980..>3322)
                     /locus_tag="PLB46_13505"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRF77588.1"
                     /translation="APEQGGGRSPFGIDCSGLTQVVYKVYGIDLPRDAYQQAEAGKIL
                     NFINEAREGDLAFFDNDEDRITHTGIILSNDQIIHASGEVRIDTIDHYGIYNKSLKKY
                     THKLRLLKRII"
BASE COUNT          959 a          642 c          625 g         1096 t
ORIGIN      
        1 taattcccgc ttaatattga ctaatgggtc ttcattttta ttgccacctc cttcgggaat
       61 ttcccaggtg tattcattaa acggataacg gtgctgaccg atgagataaa catttccttc
      121 gttgtcgatg ggaattacac cgagtgcata atttttaaat tgtactacac cataaattgc
      181 agcatggcct ccgggattta ttacatcatg ttcttccaca tgtatccacg gattatcgta
      241 aataattttt ttggataatg tttgccaggg ttgtttattt tgtgacataa ttattttgtt
      301 aaaactaatc gaataaattc agttacacct tcacctgcat ttttttgtat aaaatggata
      361 tttttactgc gcgacatttc gggtatttcg ggattaataa catataccgg aatttcgggt
      421 gaagcgtaat gtaataatcc cgctgcagga tataccacca tactggttcc aataatgacc
      481 aatatttcgg cttgttctac taatggaatg gccatttcta acatagggac ttgttctcca
      541 aaccacacaa tatggggacg taattgtgca ccgtctgctg ctacatcacc aatttggata
      601 tcgccattaa ttggtgtaat taaattggga ttttttatgc ttcgtgcttt taaaatttca
      661 ccatgtaagt gtaaaatatt tttgctgccg gcgcgttcat ggaggtcgtc gatattttgt
      721 gtgataatat ccacacgatg ctgcttttct aattcggcaa gtgcaatatg tgcgctattg
      781 ggcaatgcat tttttattcc ttttcttcgc tgattataaa aatcaagtac cagttccgga
      841 tttttattcc acccttcggg tgaggctacg tcttcaatgc ggtgaccttc ccataaacca
      901 ccggctccac gaaaggtggg aatgccgctt tcagcgctga ttccggctcc ggttaaaact
      961 acaattcgca tgggttgaag gtattgagaa aatggggatt gcgcaaacat ggcgcagttg
     1021 gttcacgcgg agatgcggag gggatgggat tatatgatga gcgatgtggt ttgtttttga
     1081 gagcgcggag cttttctcgc taaggcgcta agttatttgg attatatgat gagagatatg
     1141 gattgttttt aagcgcgcaa agctggtaaa ccaatttaat ttatcattga atttatatta
     1201 ttaattctcc gctacctccc ttctccgcgt gcaacaatta tcgtttggtt cagacggaga
     1261 tgcggatctg taaagcagtt ggacctttgc gtgcttgaaa tggtttacta tataaaacaa
     1321 acaattcacc tagcgcctta gcgagaaaca tttcttgaat tgaatggtgt ctagatttta
     1381 tgatgagatg ttatccgaat taaactttgc gtgcttgaat tggtttttta tttcgattta
     1441 gcacatacaa tagaaaactt tcctttgctc ctatgcgaga accatttcga taatttcaac
     1501 attattcagt acccatgatt atcgccttag ctaaagggat ttgatggatg atatctgtcg
     1561 caattaaatt tgagttatta ttattttcta atgctaaatc acccgctaaa ccatgtaaat
     1621 acacaccggc aatagccgcc tctttaggtg caaaactttg tgctaataaa ccactgataa
     1681 tacccgttaa tacatctcca cttcccgcag ttgccatacc cgcatttcct gtagaattaa
     1741 aatacacatt accatcggga caggcaataa tagtgtaagc acgtttatat aaaataatta
     1801 cctgatattt ttgtgctaat tgaatcatct tgtccggtaa ttcaaaccat tgctgataag
     1861 taccaaacaa tcgttcaaat tcttttggat gtggtgttaa aatagtattg gttggtatat
     1921 tattcaataa ttccggatgt tctgccatac aatttaatgc atctgcatcc actacaatag
     1981 gttgacgata tgcttgaaaa agttcagata aaatttgaaa cgttgatgag ctatttccca
     2041 aacccggacc aatactcacc gcatttattt ttttatttgt aatggttgaa tttatattct
     2101 caaaagcagt gaacataatt tcaggatgtt cattttgtaa ttgtgatgaa gtgatagtag
     2161 ataatcccgc tccggttttt aatgccgcta atccgctcat agtcgcagca ccctccatac
     2221 caacacttcc accaatagtt aatacatgtc caaaactccc tttatgcgca aataccggac
     2281 gttcgctaaa taaagactga atatcatctg cagttacaaa atgtaaatgt tgatgtaaac
     2341 ctgcacgttg tgtatcaatt aatccgatat tttgtatcat ccactggccg acatattttg
     2401 cattttccgg aaacaaaaaa gccagttttg gatattcgaa actgatggtg caatctgctt
     2461 tcacaatagg ttctaccgaa gctgaattgc tttgtaatcc cgatggaata tctacggcta
     2521 aaataatggc atctgccgca ttcatttttt caataattaa tgccggaagt ccagtaattg
     2581 atttgtttaa accagtccca aacaaacaat ctaggagaca atcgcagtca aaggaaggaa
     2641 tggtttctgc agaagaaata atatgaataa ctaaaggaaa ttgtgtttgc aggcgttgaa
     2701 aattgagggc aaaatcggct gaagattttt gcacattacc acaaatatta acctttacct
     2761 ttttacctgc ttgtaataat tgtcgcgcaa tacataaacc atcgccacca ttattgcctg
     2821 ttccgcaatt gataataaaa aattcgaaat gaggattgtc gtgtaataaa taatttgtaa
     2881 ttccttttgc ggccctttcc attaaatcgc tgctgcgaat attttgtgca actattgttg
     2941 cagcatcgag ctgcttaatt tgatgggcat caggaataat catattatac gtttgagcaa
     3001 gcgtaattta tgtgtatatt ttttgagcga tttattataa ataccataat gatcaatagt
     3061 atcaattcgc acttcacccg atgcgtgaat aatttggtcg ttacttaaaa taataccggt
     3121 atgggtaatg cggtcctcat cgttatcgaa aaatgctaaa tcgccttcgc gtgcttcatt
     3181 aatgaaattt aatatttttc ctgcttcggc ctgctgataa gcatcacgtg gtaaatcgat
     3241 gccatatact ttatacacca cttgcgttaa gcctgaacag tcgatgccaa aagggctgcg
     3301 gccgccccct tgctcaggcg cg
//
LOCUS       DAORKW010000421         3318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1588_length_3318_cov_4.961692, whole genome shotgun sequence.
ACCESSION   DAORKW010000421 DAORKW010000000
VERSION     DAORKW010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1588_length_3318_cov_4.961692"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3141)
                     /locus_tag="PLB46_13510"
     CDS             complement(<1..3141)
                     /locus_tag="PLB46_13510"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF77589.1"
                     /translation="MDLSAYTPDESEPEWYWDFGDGNYTTTYGPTNVVNAYYVAGTYT
                     VVLTANTECCTITDTIEIEVESLPGPDIFCITPVCSSEEGVQYCTSATGCSYDWIVSG
                     GFITAGLNTHCITVDWGAGPTGTISLVTTGCSPAVCSDTTTVTVPIMPNGTFNITGPL
                     VVCVNTTGNYAAPYVPGSQYTWTLVPPSGPAVILPYNIPPYQQNILFNTVGNYTLICH
                     MQNDILECYGDDTIQIKVLPEFNIQGPNTLCLNDVGNFSSNPITSCTWTTTAPGNSPQ
                     IGNGATFNFNTVGTFTVTAVATNPLAVCQDTVIYTVIVNPLPAVPVIIGPTIACAGGT
                     YVYSVSGYPASTSYQWSFIDNNFNFSFGTSNPFTITTAPTFTSGTLTLVVTDNGCTNQ
                     SVITINAPATPAPTFTVNPFVCPDAIDNYTAVLSYPGLSNVNWTISPPAAGTIVSGQG
                     TGSVAIEWHSVNPNVAQNVSITITETICLTNVGTYTQNLTIYPQPVITAAGADFCPGG
                     STLLTATGGVSYQWFDVNSAPVSPTVTSPGSYYVIGTDGNGCTAKAYVTVASLPQPVA
                     SISTPDFVLCDTNGVMLNTINLYALDNGYTFSWSPGASTANPLAVSTTGVYTVTVTDA
                     NGCTNTDSYIVECVEVDSCPLPACVCTNGPITFSQTTPNCNTITFNSNSSCSGTPLWQ
                     FGDLTGASGISVTHTYIDAGYYQVCYSNGDPGCCPPPPACTVVDIPVGANFAVEMLCN
                     TVTIFNNASVLPPYTITGYFWDFGDGFTTTVANPGPHTYTTAGPFTISCTVTASNGCT
                     ATFTQIVTPAGPDISATFATTACNGPVNFTAIVNAGNVVDWDWDFGDTYGSNVANPQH
                     IYDNPATYTVTLTGTSSNGCIDVFTGTITITAPPPPFALIYTSPNCGSVIISTAGTYS
                     TYQWYNNGNIITGATSSTYVATQSGNYTLEVTDVNGCIITSNVANVVVNPQPVIFLTV
                     PPLICRADPFSISTNVIGPYNFTWKIDATTYFAGPTLNIPAFGLLPGPHTVTVIAFDP
                     STGCADTATTVFNIKPS"
BASE COUNT         1056 a          612 c          651 g          999 t
ORIGIN      
        1 agaaggtttt atattaaata ctgtagtagc agtatctgca catcctgttg atggatcaaa
       61 tgcaataact gttacggtat gcggacctgg aagcaaacca aatgccggga tatttaatgt
      121 tggccctgca aaataagtag tggcatctat tttccatgta aaattataag gccctataac
      181 attggtactt atactgaatg gatctgccct acaaattagc ggtggaacag taaggaaaat
      241 aacaggttgc ggatttacca caacattcgc aacattactt gttattatac aaccatttac
      301 atcagtaact tcaagtgtat aattaccaga ttgcgtagca acatatgttg agcttgttgc
      361 accggtaata atattgccat tattatacca ttgataagta ctataggtac ctgctgtaga
      421 aattattacc gaaccacaat ttggtgatgt ataaattaat gcaaatggtg gcggtggagc
      481 agttattgtt attgttcctg taaatacatc aatacaacca ttcgatgatg tgccagttaa
      541 tgttacagtg taggtagctg gattatcata aatatgttgt gggtttgcca cattagaacc
      601 ataagtatca ccaaaatccc aatcccaatc taccacattt cctgcattta caattgcagt
      661 aaaattaacc ggaccattac atgcggttgt tgcaaatgtt gctgaaatat ccggaccggc
      721 aggtgtaact atttgtgtaa atgttgctgt acatccatta cttgccgtta cagtgcatga
      781 aatagtaaat ggtcctgctg ttgtataggt atgtggacca ggatttgcaa ctgtggtagt
      841 aaatccatca ccaaaatccc agaaataacc ggttatggta tatggcggta atacactggc
      901 gttattaaat atggtaactg tattacacaa catttctact gcgaaatttg cacctaccgg
      961 aatatctaca actgtgcatg caggaggtgg agggcaacat ccaggatcac cattggaata
     1021 acaaacctga taatatccgg catcaatata agtatgagta acacttattc ccgaagcacc
     1081 ggttaaatca ccaaattgcc ataatggtgt tcctgaacaa gatgaatttg aattaaatgt
     1141 tattgtgtta caatttggtg ttgtttgtga gaaagtaatt ggtccatttg tgcaaacaca
     1201 agcaggtaat ggacaagagt caacttcaac acattctact atatatgaat ctgtattggt
     1261 acaaccattt gcgtctgtta ctgtaacggt ataaacacct gttgttgaaa cggctagtgg
     1321 atttgcagtt gaagcacctg gtgaccaact aaatgtatat ccattatcta atgcatataa
     1381 attaattgta tttaacatta caccattcgt atcgcataaa acaaaatcag gtgtactaat
     1441 tgatgcaaca ggttgaggta atgatgcaac agttacataa gctttcgctg tacaaccatt
     1501 tccgtcggta ccaattacat aataactacc aggtgatgta actgttggtg aaacaggtgc
     1561 actatttaca tcaaaccatt ggtaagaaac accacctgta gcagttaata atgttgagcc
     1621 accagggcaa aaatcggcgc ctgctgctgt tataaccggt tgtggatata tcgttaaatt
     1681 ttgagtgtaa gtgccaacat tagttaaaca aatagtttcc gtaattgtga tggaaacatt
     1741 ttgcgcaaca ttaggattta ccgaatgcca ttcaattgca actgatcctg ttccctgacc
     1801 tgaaactatt gttccggctg caggtggaga aattgtccaa ttcacattcg ataatcccgg
     1861 ataggataat actgccgtat aattatcaat tgcatcaggg caaacaaatg gattaacagt
     1921 aaatgttgga gcaggagttg caggtgcatt tatagtaatt actgattggt tggtacatcc
     1981 attatcagtt actaccagcg ttaatgttcc cgacgtaaaa gttggagcag ttgttatagt
     2041 aaatggattg gatgttccaa aactaaaatt gaaattatta tcaataaatg accattgata
     2101 agaagttgaa gctggatatc ctgaaacgga ataaacataa gttccgccgg cgcaagcaat
     2161 tgttggtccg attattaccg gtacggcagg taatggattt acaattactg tatatataac
     2221 tgtatcttga caaactgcaa gcggattagt tgcaactgct gttacagtaa atgtaccaac
     2281 ggtattgaaa ttaaaagttg ctccgtttcc aatttgcgga ctatttcccg gtgctgttgt
     2341 agtccaggta caacttgtta ttggattaga actgaaattg cctacatcat ttaaacaaag
     2401 tgtgtttgga ccttgaatat taaattcagg taacactttt atttgaatag tatcatcgcc
     2461 ataacattct aaaatgtcat tttgcatatg gcagattaat gtataattgc caaccgtatt
     2521 aaataaaata ttttgctgat aaggaggaat attatatggt aaaattacag caggtccact
     2581 tggtggaact aatgtccaag tatattgtga acccggcaca taaggtgctg cataattacc
     2641 ggttgtattt acacaaacca ctaatggtcc tgtaatatta aatgtaccat ttggcataat
     2701 tggcaccgta actgttgtag tatcagaaca aactgcaggt gaacaacctg tagttactaa
     2761 cgaaattgtt cctgtaggac ctgcgcccca atctaccgta atacaatggg tatttaagcc
     2821 ggcagtaata aatccacctg aaacaatcca atcgtaactg catcctgttg cactggtgca
     2881 atattgcacg ccttcttcac tggagcatac cggagtaata caaaaaatat ccggccccgg
     2941 taaactttcc acttcaattt caatagtatc agttattgtg cagcattcag tatttgcagt
     3001 tagcacaact gtgtaagtgc ctgcaacata ataagcgtta acaacatttg taggaccata
     3061 agttgttgta taattaccat caccaaaatc ccaataccac tcaggttcag attcatccgg
     3121 agtatatgca cttaaatcca caaactcaaa gcatcggtat ctcgtggtat cctgatcata
     3181 gcgccaccaa gcccatggga tgttgtctat accgagcgtg tcacaatcgc taccatcgat
     3241 agggccgaga cggtaattag ggaaattggg tatttgaaat tttcttgaag ggagttttat
     3301 gtggtgttgt ttaaaatt
//
LOCUS       DAORKW010000422         3316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1590_length_3316_cov_5.122662, whole genome shotgun sequence.
ACCESSION   DAORKW010000422 DAORKW010000000
VERSION     DAORKW010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1590_length_3316_cov_5.122662"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1782)
                     /locus_tag="PLB46_13515"
     CDS             complement(<1..1782)
                     /locus_tag="PLB46_13515"
                     /inference="COORDINATES: protein motif:HMM:NF023400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77590.1"
                     /translation="MRAPAIWLSILILGLYSCNSDRNLIAIDSTNFEEEISVNQNLEF
                     TLNQHLFNDSLFDKWTDEELLTITPKVAGKFKITSSDQITFSPSGGFAESEKYTVAVS
                     ESIYKKTDIRLAVKKEPVMFNTPLLDLKTTNLYWSKEDATGELLLNADLLFNATVDAK
                     KIAEALVVKHAGEKKSTRINTTQTSKTITVSIHNMDPKHEERDILFALAKGISSPGSS
                     YSTPKQIQLQVEIPIIENLEIKDITAEHNGIAGTVHVYTNQQIGNSNINNLVSTQPPV
                     SLNIEKTTNGLILTSEEFQADITYTLILSEQLFGVAGGKMIDEYSQSITFGELEPMIE
                     FANGKGLYLSNKGNKNLGVRIVNVPKVKVEVIKIYENNLLAFMRDGFSYRWYEEYDSE
                     EEYWDYFDYRDVNTELFGNIVYEKEIETSKLEKYNASRVLHLDFIDKLERFNGIYVVK
                     VSSTDKRWLTDAQIVSLSDIGLIAKATEDELYVFANSIVNATPLSGVKVSFISSNNQK
                     LYTTTTDKNGVAKFSGLKKNLPDFDVNLITAESGADYNFLPFNQTIVSTSRFDVGGKR
                     LNESNMDGFLYPARDLYRPGETMYIAGI"
     gene            2098..2307
                     /locus_tag="PLB46_13520"
     CDS             2098..2307
                     /locus_tag="PLB46_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77591.1"
                     /translation="MERIAKELHLEVDAVRNYFGAEHFEAPGKIADVHFEKGLGGGVV
                     RLEDTAILQCAMRILEETGHAQTMN"
     gene            complement(2460..>3316)
                     /gene="sucC"
                     /locus_tag="PLB46_13525"
     CDS             complement(2460..>3316)
                     /gene="sucC"
                     /locus_tag="PLB46_13525"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRF77592.1"
                     /translation="EIKEFYMSVLLDREKGKNVIVYSTEGGMDIEEVAEHTPEKLHKE
                     WIDSSVGIMPFQARRVAFNLGLSGDAFKEMVKFTTSLYNAYVGNDCSLLEVNPVLKTS
                     DNKIIAVDSKCVIDNNALYRHADIAVMRDITEEDPTEVEAGEAGLNFVKLDGNVGCMV
                     NGAGLAMATMDIIKSSGGSPANFLDVGGTADAKRVETAFNIILKDPNVKAILVNIFGG
                     IVRCDRVAQGIIDAYKSIGNINVPIIVRLQGTNATEAKKMIDDSGLQVRSAILLKEAA
                     DLVKEAVG"
BASE COUNT         1000 a          642 c          572 g         1102 t
ORIGIN      
        1 aatacctgca atatacatgg tttcgccagg gcgataaagg tcacgggcag gatagaggaa
       61 accatccatg ttactttcat ttaaccgttt accgcccaca tcaaaacgcg aggtggaaac
      121 gatggtctga ttaaatggca ggaagttata atctgcacca gactcagctg taattaaatt
      181 aacatcaaag tcaggtagat ttttctttaa tccactaaat tttgcaacac catttttatc
      241 tgtagtagtg gtatataatt tctgattatt tgaactgata aaggaaacct ttactccgct
      301 caaaggagtt gcgtttacaa tggagttggc aaaaacatat aattcatctt cagtagcttt
      361 cgcaatcaaa ccaatatcag ataatgaaac aatttgagca tcagttaacc aacgtttatc
      421 tgtggatgaa acttttacta cataaatacc attaaatctt tccagtttat caataaagtc
      481 gagatgtaaa actcttgaag cattatattt ttccagctta ctggtttcga tttctttctc
      541 ataaacaata ttgccaaata attcggtgtt tacatctcgg taatcgaaat agtcccaata
      601 ttcttcttct gaatcgtatt cttcatacca cctgtaacta aacccatcgc gcataaaggc
      661 gagcaagttg ttttcataaa ttttaattac ttcaacctta actttgggca catttactat
      721 tctgacacct aaatttttat tgcctttatt actcaggtat aaccctttcc cattggcaaa
      781 ttcaatcata ggctctagtt ctccaaacgt aatactttgt gaatattcat ctatcatttt
      841 accacctgca acaccaaata attgttcaga taatataagg gtgtaagtaa tgtctgcctg
      901 aaactcttca cttgttaata tcaaaccatt tgtcgttttt tcaatattta atgaaaccgg
      961 aggttgtgtg ctaaccagat tattaatatt agaatttcca atttgctgat tggtatatac
     1021 atgaacagta cctgctatac cgttatgttc agcagtaata tcttttattt caagattttc
     1081 aataattggt atttcaacct gcaactgaat ttgtttaggc gttgaatagg aactacctgg
     1141 tgaactgatg cctttagcta atgcaaataa aatatctcgt tcctcgtgtt tcggatccat
     1201 attgtgaata gataccgtaa ttgtttttga agtttgagtc gtattaattc gagtcgattt
     1261 tttttcacct gcgtgtttca ccactaatgc ttctgctatt tttttcgcat ctacagttgc
     1321 gttaaataat aaatcagcgt ttaataataa ttcacctgtg gcatcttctt tggaccaata
     1381 taaattagtt gtttttaaat ccagtaatgg tgtattaaac attaccggtt cctttttaac
     1441 tgctagccgt atatccgttt ttttataaat tgattcgctg acagccaccg tatatttttc
     1501 actttcggca aagccaccgg aaggggaaaa tgtgatttgg tcgcttgaag ttattttgaa
     1561 ttttccggca acttttggcg taatggtaag tagttcttcg tctgtccatt tgtcaaataa
     1621 tgaatcgttg aacagatgct ggttgagggt aaattccagg ttctgattca cactaatttc
     1681 ttcttcgaaa ttggtagagt caatagcaat aaggtttctg tcggaattac atgaatacaa
     1741 accgagtata aggatactca gccaaatagc aggggcccgc atagtgttgg gaggttttga
     1801 gtaataggtg aaatgattac atagtaaaac gataaatcca tcgctgaatt gtattgtaat
     1861 tatactattt ttttgggaat tgctaggtag tagattaata actgctatat gtatctgtat
     1921 aagtgtgtgg ggattatggc tgtttataaa aaaagtggcg aaataatttg gaaatcttat
     1981 aaaacatagc taaaatcgta tcgtcttacc gatatattgt ttaactaaaa tattataact
     2041 atggtgatta cattcgacga gttacgacac atcaaagaca cgttaccaca cggcagtatg
     2101 gagcgtatcg ccaaagaact tcatttagaa gtagatgcag tgagaaatta tttcggagcc
     2161 gaacattttg aggccccggg caaaattgcg gatgtgcatt ttgaaaaagg tttgggcgga
     2221 ggagtagtca gactggaaga cacggccata cttcagtgtg ccatgcgcat attggaggaa
     2281 acaggccatg ctcaaacaat gaattgattc ttttcatact tgaattataa cccattggtc
     2341 gtcagattaa tgggtttttt gttttaagtc cctgtcttat agataaggat tcccgtatca
     2401 atctcctaat aacaaaaaaa gcatccctaa ggatgctttt tcatgtataa tatagttgct
     2461 tagccgacag cttctttaac caaatcagct gcttctttta acagaatagc tgaacgcact
     2521 tgtaaaccac tgtcatctat catctttttc gcttctgtag cattagttcc ttgtaaacga
     2581 actatgattg gaacgttaat attaccaatt gatttataag catcgataat accttgtgca
     2641 accctgtcgc aacgaacaat acctccaaaa atattcacca aaatggcctt tacgttcggg
     2701 tcttttaaga tgatgttaaa ggctgtttct acgcgtttag cgtctgcagt acctccaaca
     2761 tcgaggaagt tagccggact accaccgctg gatttaataa tatccatcgt cgccatagcc
     2821 aaacctgcac cattaaccat acaacccaca tttccatcca atttcacaaa gtttaaaccg
     2881 gcttcacctg cttccacttc tgtaggatcc tcttctgtta tatcgcgcat tacagcgata
     2941 tcagcatggc gatataaagc attgttatca atcacacatt tggagtcaac agcaataatt
     3001 ttgttatctg aggtttttaa aaccggattt acttctaaaa gtgagcaatc gtttccaaca
     3061 taagcattgt aaaggctggt agtgaatttt accatttctt tgaacgcatc accgctaaga
     3121 ccaaggttaa atgcaaccct gcgcgcctga aacggcataa tacccacact gctgtcaatc
     3181 cactctttat gtaatttttc aggtgtatgt tctgccactt cttcaatgtc cataccacct
     3241 tctgtagaat atacgattac gtttttacct ttttcacgat caagcaacac actcatgtaa
     3301 aactctttaa tctcgg
//
LOCUS       DAORKW010000423         3313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1592_length_3313_cov_5.231737, whole genome shotgun sequence.
ACCESSION   DAORKW010000423 DAORKW010000000
VERSION     DAORKW010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1592_length_3313_cov_5.231737"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLB46_13530"
     CDS             complement(<1..204)
                     /locus_tag="PLB46_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77593.1"
                     /translation="MKQLTILVISCLISTTAFNQSSQQFSGGEYTMAPLDEMSPEQRA
                     TIFQMLEENEAKLQAEGKLPLVYN"
     gene            complement(492..3107)
                     /gene="alaS"
                     /locus_tag="PLB46_13535"
     CDS             complement(492..3107)
                     /gene="alaS"
                     /locus_tag="PLB46_13535"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRF77594.1"
                     /translation="MTSQEIRQHFLKFFESKQHKIVDSAPIVVKNDPTLMFTNAGMNQ
                     FKDLFLGNKPIQYPRIADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDYFK
                     KESINWGWELLTEVYKLDKSRLYVTVFEGDAAENLEFDQEAYDVWKSLIDETHILKGN
                     KKDNFWEMGDTGPCGPCSEIHYDMRSDAERAAVDGATLVNMSDPQVIEIWNHVFMQFN
                     RKADKSLVELPAKHIDTGMGFERLVRALQGKSSNYDTDVFTPLIKKIEQLSGHSYGKS
                     EQTDIAMRVIADHIRAVSFCIADGQLPSNTGAGYVIRRILRRAIRYGFSFLGFKKPFF
                     NELVPVLADQFNHVFSELHAQRELVAKVIFEEETAFLRTLENGIKRLTDVMSEAKSKN
                     QTSISGVVAFELYDTYGFPFDLTTLITRENGLDTDEAGFNVEMQKQKDRSRNASGMET
                     GDWIIVNDDTKIEFVGYTDLACEANLVKMRSVKVKNEMQYQVVLNKTPFYAESGGQTG
                     DTGFISVNADVIRVLDTKKENDLIIHFTDQLPQQNAAKIFAQVDAKRRTDITYNHTAT
                     HLLHAALRSVLGTHVQQKGSYVGPDRLRFDFSHFSKITDAELQQIELMVNKKIRENIP
                     VVTKVMPIAEAQQLGAMMLFGEKYGETVRVVIADENYSKEFCGGTHVGATGEIGYCKI
                     VSESAVAAGIRRIEAITGEAVGKFIDEQLTTIQQITELTKGTDIVKSIGSLVEENSTL
                     KKEIEKYQLMQLNQIKNDLLAKKETFGDVQVIRAIVDLPNADLIKKLAYELRNETTNL
                     FCVLGAEIQAKASLTIMISDELVAAKNMDASKLIRELGKEIEGGGGGQKFFATAGGKK
                     PEGLQKVLQMSVAGL"
BASE COUNT         1001 a          679 c          517 g         1116 t
ORIGIN      
        1 attatatacc aatggtaatt ttccttcggc ctgtaatttg gcttcgtttt cttccaacat
       61 ttgaaaaata gttgcccttt gttcagggga catttcatct aaaggtgcca tggtgtattc
      121 accaccacta aattgttggg atgactgatt aaatgctgtt gtgcttataa ggcagcttat
      181 aaccaggata gtaagttgtt tcattggtct attttgtgat aaaaatagca taaaaacctg
      241 aaaatgcgcc catgaattaa caattcgata cctactagtt tttgtcaaaa ttgacatccc
      301 acttgatttt gattaatcat cacttcaaca aaactcagga ttatttaaaa agactttgag
      361 tttcgcaatc catgggctat tgcatgatcc atttaaccgc acttcaatct ttgtaagcaa
      421 caaaaacgac atacaatagc ataaaaaaac cggatgaata ttcacccggt tttaaattat
      481 ttaaaaaaaa tttataatcc tgcaaccgac atttgcaaaa ccttttgtaa cccttcaggt
      541 tttttaccac cagcagttgc aaaaaatttc tgaccacctc caccgccttc aatttctttg
      601 cctagttcgc ggattaattt agacgcatcc atatttttag cagccactaa ctcatcacta
      661 atcataatgg ttaacgatgc cttagcttga atttcggcac ctaacacaca aaataaatta
      721 gtggtttcgt tgcgtaattc gtaagccagt tttttaatta aatccgcatt cggtaaatca
      781 acaatggcac gaataacctg cacatcacca aatgtttctt ttttggcaag taaatcattt
      841 ttgatttgat ttaattgcat caattgatat ttttcgattt ccttttttaa agtactgttt
      901 tcttccacta aactgccaat actttttacg atatccgtgc ctttagtcag ttcagtaatt
      961 tgttgaatgg tagttaattg ttcatcaata aatttaccta cagcttcacc tgtgatggct
     1021 tcaattctgc gaataccagc ggctaccgcg ctttcactaa ctattttaca ataaccaatt
     1081 tcgccggttg cgccaacatg tgtaccaccg caaaattctt tgctataatt ttcatctgca
     1141 attacaacac gcactgtttc gccatatttt tcaccgaata acatcatagc acctaattgt
     1201 tgtgcttcag ctattggcat tactttagta acaaccggaa tattttcgcg aatttttttg
     1261 ttgaccatta attcaatctg ctgtaattcc gcatcggtga ttttagaaaa atgcgaaaag
     1321 tcgaaacgca atctgtccgg cccaacataa cttccttttt gttgcacatg cgtacctaaa
     1381 accgaacgca atgctgcgtg taataaatgt gttgcggtat gattgtaagt aatatctgtg
     1441 cgacgtttag catcaacctg cgcaaatatt ttagctgcat tttgttgtgg caactggtcg
     1501 gtgaaatgaa taattaaatc attttctttt ttagtatcca atacgcgaat tacatctgca
     1561 tttacagaaa taaaaccggt atcacctgtt tgtccgccgc tttcagcgta aaatggtgtt
     1621 ttatttaata ccacctgata ttgcatttca tttttcacct taaccgaacg cattttaacc
     1681 aggttggctt cgcaagctaa atctgtataa ccaacaaatt caattttcgt atcatcattt
     1741 acaataatcc agtcgccggt ttccattccg gaagcattgc ggcttctgtc tttttgtttt
     1801 tgcatttcaa cattaaatcc ggcttcatct gtatctaaac cgttttcacg tgttattaat
     1861 gtggttaaat cgaatggaaa tccataggta tcatataatt caaaagcaac aacacctgaa
     1921 atggaagtct gattttttga tttagcttcc gacattacat ctgttaaacg tttaatcccg
     1981 ttttctaagg ttcttaaaaa tgctgtctct tcctcaaaaa ttactttagc aactaattcg
     2041 cgctgtgcat gtaattcaga aaatacatga ttaaactggt cggctaaaac aggtactaat
     2101 tcattaaaaa atggcttctt aaatcctagg aaagaaaatc cgtaacgaat agcacgacgt
     2161 aaaattcttc tgataacata tcccgctccg gtgttagatg gtaattgtcc atctgcaata
     2221 caaaaactca ctgcacgaat atggtcagca ataacacgca tagcaatatc agtttgttct
     2281 gatttaccgt aactatgtcc gcttagttgt tcaatttttt taatgagtgg tgtaaataca
     2341 tctgtatcat aattcgacga tttaccttgt aatgcgcgca ccaatcgctc aaatcccata
     2401 ccggtatcga tatgttttgc aggtaattca accaacgatt tatctgcttt gcgattaaat
     2461 tgcataaata cgtgattcca gatttcaata acctgtgggt cactcatatt tactaaggta
     2521 gcaccatcaa ctgcagcgcg ttcagcatca gaacgcatat cgtaatgaat ttccgaacat
     2581 ggtccgcatg ggccggtatc acccatttcc caaaaattat cttttttatt accttttaaa
     2641 atatgtgttt catcgataag tgatttccaa acatcgtaag cttcctgatc aaattccaga
     2701 ttttcagcag catcaccttc aaatacagtt acatataatc ggcttttatc taatttgtac
     2761 acttcagtaa gtaattccca tccccaatta atactttctt ttttgaaata atcgccaaaa
     2821 ctccagttgc ctaacatttc gaacatagta tggtgataag tatcaacacc aacttcttca
     2881 aggtcgttat gttttccgga aacgcgtaaa catttttgag tatcagcaat acgtggatat
     2941 tgaatgggtt tattaccaag aaataaatct ttaaactgat tcataccggc gttagtaaac
     3001 attaacgttg ggtcattctt aaccacaatt ggcgctgaat caacgatttt atgttgttta
     3061 ctttcaaaaa atttcaaaaa atgctgacga atttcctgcg atgtcataat agttcctgtt
     3121 ccttcgtttt atgttttgta aaatattaac tattttcgtg tccctccaaa aagcacacag
     3181 ccattaaatt aaggtgcaaa attactgaat cacggccgca tggcgaagaa ggtaaaatat
     3241 ttttataatc cagcgaatct caggttcgaa aagcacgtaa ctccgcgttg ggtatggact
     3301 ttacggatat tgg
//
LOCUS       DAORKW010000424         3307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1597_length_3307_cov_3.666974, whole genome shotgun sequence.
ACCESSION   DAORKW010000424 DAORKW010000000
VERSION     DAORKW010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1597_length_3307_cov_3.666974"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PLB46_13540"
     CDS             complement(<1..1094)
                     /locus_tag="PLB46_13540"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRF77595.1"
                     /translation="MMRIVFFVSAIVCLSYFTSYGQEPYKPYRIADDIVLDGKLDEQV
                     WQLTPSEDVFMQQSPQAGAPASEKTIYRIVYNDDYLYVGIICFDSEPDNLTRLALNRD
                     YALGDDDGTGVIIDTYRDKTTGLGFLANTLDARWDASFSQDGTSNNDSYNTYWDAVTA
                     IQPYGYSTEYRIPFSSLRFETKSEVIMGIRISRLIKRKNELITSPPCDPSTQGAWSNL
                     SFAREVVFTNLVSRTPFYISPYAIANFNRFNILNADSSAYVAQTEFMQRKHFDDIEIV
                     DKIISNIGIDAKYGITKNLTLDLTINTDFAQAEVDDRIINLSKYEVNLPEKRSFFLES
                     ANNLSFGFPSGNTLFISRKIGRENGLIVPI"
     gene            complement(1300..2538)
                     /locus_tag="PLB46_13545"
     CDS             complement(1300..2538)
                     /locus_tag="PLB46_13545"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HRF77596.1"
                     /translation="MKYFVMGFGFLFTATALFAQQPEKAEYREPKGNYYQDVILPDVN
                     KSESTKATKDARLFKIDGSGINAPKDPKSYNTIWHGNPVSQGNTNTCWCFSTISFFES
                     ETKRTSGKEIKLSEMYVVYWQYVERAKYFVAHRGDMYFGEGSETNGTTNMIKQYGIVP
                     YDNYKGKTSTASFYNHETMFKELQAYLQGVKDNNNWNETEVVNSVKAILNFYMGEPPT
                     SVTWENKNYTPIEFSKNILKLDMNNYVDFMSLMQKPFYTQAEYDVPDNWWNDATYYNL
                     PATDFMSTIKTALKNGYSISIGGDVSEPGWDSYNNIAVIPTWDISSEYINDAARQLRF
                     NNGSTTDDHAMHLVGYKEQDGKTWFLYKDSGSGSRNCGAASPSFGYLYVSEDYVKLKM
                     MSFTVHKDAVKPILEKFAVK"
     gene            complement(2627..>3307)
                     /locus_tag="PLB46_13550"
     CDS             complement(2627..>3307)
                     /locus_tag="PLB46_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77597.1"
                     /translation="TATSAISKEIREQYGCEPFLNKHATYDDFLRKASPAEKKYQAIG
                     SVRNPLDQTVSMYFKYKNDLDQRFSSGRKRPGKWLRKSLAKNRDAVRYQFIIQNNADF
                     ETYFLKFFKWPYSNWSIIHHKKMAHLIRFEHLVEDYRKVFTAVGLPITRDLPQANKTP
                     EKKADFWSYYTSDEAKRRAKFVFGPFMRYWGYSFPESWASIKEPMHAQLLYNFLNFFR
                     RIYWQFLR"
BASE COUNT         1001 a          599 c          602 g         1105 t
ORIGIN      
        1 attattggga ctattaatcc attttcgcgc ccaatttttc ttgaaatgaa taaagtattt
       61 cccgaaggaa atccaaagct taggttattg gcggattcca aaaagaaact tcgtttttcc
      121 ggtaggttta cttcgtactt ggaaaggtta ataatgcggt catcaacttc agcctgtgca
      181 aaatctgtat tgattgttaa atctaaagtc aaatttttag tgataccata tttagcatcg
      241 ataccaatat tgctgattat tttatctaca atttcgatat cgtcaaaatg tttgcgctgc
      301 ataaattcag tttgcgcaac ataagccgag gaatctgcat ttaagatatt aaaccgattg
      361 aaattagcaa ttgcataagg actaatataa aaaggtgttc tgcttacaag gttcgtaaaa
      421 actacttctc ttgcaaacga taggttagac cacgcacctt gtgttgaagg gtcacaggga
      481 ggtgaggtaa tgagttcgtt tttacgttta attagccgtg aaattcgaat acccataata
      541 acttcacttt ttgtttcaaa tcgaagggaa gaaaatggaa tacgatattc tgtagaataa
      601 ccataaggtt gaatggcagt aactgcatcc caataagtat tgtaggagtc gttattcgat
      661 gtgccgtctt gagaaaagga ggcatcccaa cgggcatcta aagtattagc taaaaaaccc
      721 aagcctgttg ttttatccct gtaggtatct ataattacgc ctgtgccatc atcatcgcct
      781 aatgcataat ctctgtttaa tgccaatcgt gttaaattat caggttcact atcaaaacaa
      841 attatgccta cgtataaata atcatcatta tatacaatgc ggtatatcgt tttttctgat
      901 gccggagctc cggcttgagg cgattgctgc atgaatacat cttcagatgg cgtaagttgc
      961 caaacttgtt catctaattt accatctaac acaatgtcat cagcaatacg atagggttta
     1021 tatggctctt gtccgtagga agtaaaatag gataagcaaa caattgcaga aacgaaaaac
     1081 acgatgcgca tcatgaatta ttgggttgtt ttaagtatgc ctgattgaaa tgtcaggtat
     1141 aaaattactg atttcctata taccctttta attttaccta tacaaactta aaatcagctt
     1201 gcaatccgat ttggtttatt tataaaattg cctgttgtag ggaataacaa agcgccacat
     1261 gttataattt agataacatg tggcgcttta gaagtggtat tatttaacag cgaatttttc
     1321 taatataggt tttacagcat ctttatgtac agtaaatgac atcattttta attttacata
     1381 atcttcgctc acatataagt aaccaaagct gggacttgca gctccgcaat tgcgtgaacc
     1441 ggaaccactg tctttatata aaaaccaggt tttcccgtcc tgttctttat atccaactaa
     1501 atgcatagca tggtcgtcgg tagtagagcc attattgaaa cgtaactgac gggcggcatc
     1561 attaatgtat tcgctgctaa tatcccaagt tggaattact gcaatattat tgtagctatc
     1621 ccaacccggt tctgaaacat caccacctat gctgatactg taaccgtttt ttaatgcggt
     1681 tttgatagtg gacatgaaat ctgtagccgg taaattgtaa taggtagcat cattccacca
     1741 attatcgggg acatcatatt cagcttgtgt ataaaatggt ttttgcatta aactcataaa
     1801 atctacataa ttattcatgt cgagttttaa tatgtttttt gagaattcaa ttggcgtata
     1861 atttttattt tcccacgtaa ctgatgtagg tggttcaccc atataaaaat tcaggattgc
     1921 tttaacggaa tttactactt cagtttcatt ccagttatta ttgtctttca caccttgtaa
     1981 ataggcctgt agttctttaa acattgtttc atggttataa aacgatgcag tcgaagtttt
     2041 ccctttataa ttgtcgtaag gaacaatgcc gtattgttta atcatattgg tagtgccgtt
     2101 tgtttcactg ccttcaccaa aatacatatc gccacgatga gcaacaaaat atttagcgcg
     2161 ttcaacatat tgccaataca ctacatacat ttcacttaat ttaatttctt ttccgcttgt
     2221 gcgttttgtt tcgctttcga aaaaagaaat agttgaaaaa caccaacagg tattggtgtt
     2281 gccctgacta actggattgc catgccaaat cgtattatag gattttggat cttttggtgc
     2341 attaattcct gagccgtcta ttttaaacaa acgggcatct tttgttgctt ttgtagattc
     2401 gcttttgttg acatcaggta aaataacgtc ctgataataa ttaccttttg gctcgcgata
     2461 ttcagctttt tcgggttgtt gggcaaataa tgcagtggct gtaaaaagaa agccaaagcc
     2521 catcacaaaa tatttcatgt tgtaaagttt agtcgtctca aaattacctg tttggtttcg
     2581 agaaatacta aaaacggaag ctaaaatctg ttaaaaaaca ataggattat cttaaaaatt
     2641 gccaataaat tcttcggaag aaattcagaa aattatagag tagttgggcg tgcataggtt
     2701 cttttataga tgcccaactt tcaggaaatg aatatcccca atagcgcata aatggaccaa
     2761 atacaaattt ggctctgcgt tttgcttcat ctgaagtata ataactccag aagtccgcct
     2821 ttttttcagg tgttttgttt gcctgaggca ggtctcttgt tataggaagt ccaacagccg
     2881 taaatacttt gcgataatct tcaacaagat gttcgaagcg aattagatgg gccatttttt
     2941 tgtggtggat gatactccag ttgctataag gccacttaaa aaatttcaaa aagtaagttt
     3001 caaaatcagc gttattctga ataataaatt gataacgcac cgcatcccga ttttttgcca
     3061 acgattttct caaccatttg cccgggcgtt ttctgcctga tgaaaatcgt tggtctaaat
     3121 cgtttttgta tttaaaatac atgctcaccg tttggtccaa tggattgcgt acgctgccaa
     3181 tagcctgata ttttttttca gccggcgaag ctttgcgcag gaaatcatcg taagtggcgt
     3241 gtttattcaa aaaaggctca caaccatatt gctctcttat ttccttggaa atagcactag
     3301 tggctgt
//
LOCUS       DAORKW010000425         3306 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1598_length_3306_cov_4.599815, whole genome shotgun sequence.
ACCESSION   DAORKW010000425 DAORKW010000000
VERSION     DAORKW010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1598_length_3306_cov_4.599815"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLB46_13555"
     CDS             complement(<1..741)
                     /locus_tag="PLB46_13555"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRF77598.1"
                     /translation="MEKPTFNEAIKFWIKLGWISFGGPAGQIAIMHEYLVDKKKWISD
                     KKFLHALNYCMLLPGPEAQQLATYTGWLLHGTRGGLAAGIFFVLPSVLILLLLSIIYV
                     TFGDLPWVSALFQGLKPAVVAIVILAMIKIAKKSLVSNYHYIIAILSFIAIYFFNISF
                     PLIILGTIIFALVHHKWIKKSSNPSNQKSSNNQDEHGYYLTTSSVIPHTGFQLKRLIR
                     QIGLFAILWVAPLLLFYLLSSNFPFWQQL"
     gene            complement(820..1221)
                     /locus_tag="PLB46_13560"
     CDS             complement(820..1221)
                     /locus_tag="PLB46_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77599.1"
                     /translation="MRNKVTLTLIATVGLIFSACNPYSTWEKSVVNNSSKDIILYVSD
                     SGGGFQFTDSIIIPPAAQEIIYSFGDVVKSETPECDLYISRLTIATDSGYIVTKDIQS
                     ADNWVSDTKDADKGFDHSCVYTVSDSDIIED"
     gene            complement(1324..>3306)
                     /locus_tag="PLB46_13565"
     CDS             complement(1324..>3306)
                     /locus_tag="PLB46_13565"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HRF77600.1"
                     /translation="EIKIPYSAVRFPSKEKQDWGLQLIRSINSTGEYIIWSPVDPNIM
                     GVVNQWGSYTGLENIVPPLRLSFSPYLTAGTQWTPVDEEGETVIYEQNNILNGGLDVK
                     YGINESFTIDATLIPNFGEVQSDNVILNLSPFEQQFNERRPFFTEGTEIFNQTFNFIG
                     DNMFYSRRIGGVPFNYYSAYDATKRHEVVIENPYETGLINATKFSGRTDKNLGIGIFN
                     AIATEEYAVLRDTVSGEERTVLTNPLTNYNMIVFEQALKNNSKVSITNTNTLRDGIYR
                     DANVTQGLFDITNAKHTWNYWGFGNYSQVYEPYYDENNVIVDDPTTGFNYIAGVSQFT
                     GKWNFNLSHALISPTYDHTDMGIQYGYNFINNYFGFNYNNQEPKKGIFYNYNAWGGFA
                     YNLQYDPLEYQEINYNLGFNGQFKNFWNVGGNLYSKPIWWYDFYEPRYEGRKYYHAPF
                     TFINVWGGSDYRKDLSFNWNLSFGESPLANDPYFGGGQYITWNVNDKFSISHGLGLSK
                     DHNNFGFVDFDAADNSIFGRRHVTTIDQEVSFKYIFGPKMNILARARQYWGKVVYHEF
                     YELLADGNLGETDFEGNYDINFNIFNVDFVYAWEFAPGSYFNLIWKNNIFQYDDIRVD
                     DYFDNLRKTFETPQTNGLSIKLIYYLDYQYLIKKNI"
BASE COUNT         1089 a          622 c          541 g         1054 t
ORIGIN      
        1 gagttgttgc caaaaaggaa aattactgct gagcaaataa aataacagga gaggggctac
       61 ccacaaaatc gcaaataagc cgatttgtct tataagtctt tttaattgaa atcccgtgtg
      121 tggaattacg cttgatgttg ttaaataata accatgttca tcttgattat ttgagctctt
      181 ttgattggat ggattgctgg atttttttat ccatttgtga tgaactaaag caaaaataat
      241 tgtacctaat attattaacg gaaaggaaat attgaaaaaa taaatggcta taaaacttaa
      301 tatggcgatg atgtagtgat aatttgaaac cagcgacttc tttgcaattt taatcattgc
      361 taaaataacg atggccacaa ctgcaggttt caatccttga aataaagcac tcacccaggg
      421 taaatcacca aaagtgacat aaataatact gagtaacaat aatatgagca ctgatggcaa
      481 aacaaaaaaa atgcctgcgg ctaatccacc cctagtgccg tgcaacagcc atccggtata
      541 ggttgcaagt tgctgagctt cagggccggg caacaacata caataattta atgcatggag
      601 aaatttttta tcgctgatcc atttcttttt gtctaccaaa tattcatgca tgatggcaat
      661 ttgtccggct ggaccgccaa aacttatcca tccgagttta atccaaaatt tgattgcctc
      721 attaaaggta ggtttctcca taatactaac ttagctctcc aaaaaacaaa accgggaata
      781 agaatatacc cggtttgtta gattccttta tagataaatt taatcttcaa ttatatcact
      841 atcagataca gtatacacac atgaatggtc gaaacctttg tctgcatctt ttgtatcgct
      901 aacccagtta tctgctgatt gaatgtcttt agttacaata tagcctgaat cagttgcaat
      961 tgttaatctg gaaatatata agtcacattc cggtgtttct gacttaacaa catcaccaaa
     1021 tgaataaata atttcttgcg ctgccggagg aataataatg ctatcagtaa attgaaaacc
     1081 accaccggaa tcagaaacat ataagatgat atctttcgag ctattgttaa ctactgactt
     1141 ctcccaggtt gagtaagggt tgcacgcaga aaatattaaa ccaactgtgg caattaaggt
     1201 aagggttact ttatttctca tatgacaagc taaaagtaaa actgtttacc gaaataacca
     1261 aaaagacaag ggtttcagcc attaaacaga ctaaaaccct tgtcacaaaa gtaagtgtaa
     1321 atattatata tttttcttta taagatattg ataatcaagg taatatatca attttattga
     1381 caaaccattg gtttgtggtg tctcaaaagt cttgcgcaaa ttgtcaaaat aatcgtcaac
     1441 ccgaatatca tcgtactgaa agatattatt cttccagatg aggttaaagt agcttcccgg
     1501 agcaaattcc catgcataca caaagtctac attaaatatg ttgaagttaa tatcgtaatt
     1561 accctcaaaa tcagtttcgc cgaggttgcc atctgctaac aactcgtaaa attcatgata
     1621 gaccactttt ccccaatatt gtctggctct ggcaaggata ttcattttag gcccaaaaat
     1681 gtatttgaac gatacttcct ggtcaatagt agtaacatgt cttctgccaa aaatactatt
     1741 atccgcagca tcaaaatcta caaatccaaa gttattgtga tccttactca accctaaacc
     1801 atgcgaaatt gaaaatttat cattgacatt ccaggtaatg tattggccac caccaaaata
     1861 ggggtcattt gccaatgggg attcgccaaa actcaggttc caattaaaag ataaatcttt
     1921 tcgataatca cttccacccc aaacattaat aaatgtaaat ggcgcatgat aatatttacg
     1981 gccttcatat cttggctcat aaaaatcgta ccaccaaatg ggtttagaat ataggtttcc
     2041 accaacattc cagaagtttt taaattgacc gttaaagccc aaattgtaat taatttcctg
     2101 gtattccaaa gggtcgtatt gcaaattata ggcaaatcct ccccaggcat tgtaattata
     2161 aaatatccct tttttgggct cctgattatt gtaattaaat ccaaaataat tattgatgaa
     2221 attatagcca tactggatac ccatatcagt gtggtcgtag gttggactaa taagtgcatg
     2281 acttaaattg aaattccatt ttccggtaaa ctgtgaaaca cctgctatat aattaaagcc
     2341 ggttgttggg tcatccacaa ttacattatt ttcatcgtaa taaggttcat atacctgact
     2401 atagttgcca aaaccccagt aattccaggt atgtttggcg ttggttatat caaataaacc
     2461 ttgtgtaaca ttggcatcgc gataaatacc atcacgcagt gtgttagtgt ttgttatact
     2521 tacttttgaa ttatttttta atgcctgctc aaatacaatc atgttgtaat tggtgagagg
     2581 attagttaaa actgttcgtt cttcgccgct cacagtatca cgcaatacgg catattcttc
     2641 cgtcgcaatg gcattaaaaa taccaatacc taaattttta tctgttctgc ctgaaaattt
     2701 agtggcattt attaaacctg tttcatacgg attttcaatc accacttcat gacgtttagt
     2761 agcatcgtat gcgctgtaat aattaaacgg aacaccacca atacgacggg aataaaacat
     2821 attgtcgcca atgaaattaa atgtctgatt aaatatttct gtaccttctg taaaaaatgg
     2881 tctgcgctcg ttaaactgtt gttcaaatgg cgagagattt aatattacgt tatcactttg
     2941 tacttctcca aaattgggaa ttaatgtagc atcaattgtg aaactttcgt tgataccata
     3001 ttttacatct aaaccaccat ttaaaatatt attttgctcg tatatcactg tttcaccttc
     3061 ttcatcaaca ggtgtccatt gtgttccggc tgttaaatag ggagaaaacg ataaacgcaa
     3121 tggtggaaca atattttcta atccggtata actgccccat tgatttacca cacccataat
     3181 attagggtct acaggactcc agataatgta ttcaccggtg ctgtttatgc ttcttattaa
     3241 ttgtaatccc caatcttgtt tttcttttga tggaaatcgc acagctgaat aggggatttt
     3301 tatctc
//
LOCUS       DAORKW010000426         3305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1600_length_3305_cov_4.871077, whole genome shotgun sequence.
ACCESSION   DAORKW010000426 DAORKW010000000
VERSION     DAORKW010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1600_length_3305_cov_4.871077"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..568)
                     /locus_tag="PLB46_13570"
     CDS             complement(161..568)
                     /locus_tag="PLB46_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77601.1"
                     /translation="MKRVLTAYFTAVIVLLAFSYAALYIAIYFFPGVAEQYFDPVYSS
                     SEKRTWMYFAHPFIVSLALKYFWQRFRHMFKGGPIRRGAEVGLIYGLIAVLPAMWITF
                     SGISISISVVATWILYGTLQGIIAGVIYAKMNP"
     gene            754..1335
                     /locus_tag="PLB46_13575"
     CDS             754..1335
                     /locus_tag="PLB46_13575"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRF77602.1"
                     /translation="MKCFKLITFVILIVPAMVLRAQSSYVLAGDHKITIEGSSNVHDW
                     EEVAQTASGDASVLWNPDASFAIQKFNLKVSVKSIKSDKGSIMDDKTYDALKGAKYPY
                     ITFKMLSVKSMTKSGSGYALKVNGELTIAGVTKNVDINGTVSVKENGKLLIETSKALK
                     MTDFGIDPPTAMMGAMKVGNDITIKFKLNYTLK"
     gene            1372..2775
                     /locus_tag="PLB46_13580"
     CDS             1372..2775
                     /locus_tag="PLB46_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77603.1"
                     /translation="MKNSINQKLLALALTALSVLSLNAQNNQNDKINYDRPYDKAGIN
                     VFETTKKDAVTFTGFQLKISGGFTQQYQALSHENTADVILKDNTLTATAGDSVNINAL
                     YPMTNGFNLATANLILTAQLADGIAIKLENYMSARHHNEFWVKGGYIQMDKLPFFGNP
                     QWFADNFTVKVGQMEVNYGDAHFYRTDNANGIYNPFVGENIMDAFATEISGELYYKPE
                     NGIIAMLGLSSGLLNADLSIFKDPATGDTIGRKPSVYAKLGYDKQLYDDLRLRLTGSV
                     YANGGTSRNTLYGGDRAGSRYYLAGEAQYYMSGTTLTAATPTNKAFSGRIDPGFSSNL
                     MSIMINPFVKFHGLEVFGTYEMSSGSAGLSNVLDSVDRSFSQIAGSVVYRFLPEEQCY
                     VGVRYNTVTGRLYGYSDDDGDVNVNRLQVAAGWFPTENLLLKIEYVDQKYDNFKTTDV
                     RNGLEFKGVMVEASVGF"
     gene            complement(2851..>3305)
                     /locus_tag="PLB46_13585"
     CDS             complement(2851..>3305)
                     /locus_tag="PLB46_13585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77604.1"
                     /translation="IYDQDGQIFQSVSFEDVAPYPLSPDGGGTALQIVSIGENMNKPS
                     NWMESCPAGTPGSLLVMPCITGIEEQEIDVIMKLQPNPATSVLHITFTRPLNACQLSV
                     YDITGKLMLKHNIQQSNLLLDVANYNSGMYVIEVVAENEHFTNTFIKQ"
BASE COUNT         1141 a          554 c          589 g         1021 t
ORIGIN      
        1 aaccacatcg cacatttatt aaatctaaaa aaaacttcgc gcctttgcga gaaacaaaca
       61 tcaactgtta taaatttagt ttaccaacaa ataaaaaacc cgcaatgcgg gtttttttat
      121 gcgtgagcat atgaacgttg aaagtattat ttcgaagcga ttatggattc atttttgcat
      181 aaataacacc tgctataata ccttgtaaag tgccatacaa aatccatgtt gcaactaccg
      241 aaattgaaat ggaaatacca ctaaatgtaa tccacatagc aggtaatacc gctattaaac
      301 cataaattaa ccccacttca gctccacgac gaataggacc acctttaaac atgtggcgga
      361 agcgttgcca gaaatatttt aaagcgagcg aaacaataaa tggatgagca aaatacatcc
      421 aagttcgttt ttcactactg gaatatacag ggtcgaaata ttgttcggct acacccggga
      481 agaaataaat ggcaatgtat aatgcggcat aactgaatgc cagtaacact attactgcag
      541 taaaataagc tgtaaggacg cgtttcatat aataagtatt tatttaatta atatatggtt
      601 aattaatata tataatctat tttttgtttg atgtatattt atagtgcagt gaatgcacca
      661 caattattcc acaaagttag atagtgtcaa gctacaaaag gtgactgaaa ttactttgca
      721 ctgtataaag attacagctt taaatcacct attatgaaat gcttcaaact aatcaccttt
      781 gttattttaa ttgttcctgc catggttttg cgggcacaaa gtagttatgt gcttgccggc
      841 gaccataaaa taaccataga aggttcttcc aatgtacatg attgggaaga agtagcccaa
      901 acagcttcag gcgatgcatc tgtgttgtgg aatcctgatg cctcatttgc tatccaaaaa
      961 tttaatctta aagtgagcgt aaaatctatt aaaagcgata aaggctctat aatggatgat
     1021 aaaacttacg atgcattaaa aggagccaaa tatccttata tcacttttaa aatgttgtca
     1081 gtaaaatcga tgacaaaaag tggtagtggt tatgcactta aagtgaatgg agaactcacc
     1141 attgccggtg ttactaaaaa tgtagatatc aatggtactg tatccgtaaa agaaaatggt
     1201 aaactcttaa ttgaaacatc aaaagcatta aaaatgaccg actttggtat tgaccctcca
     1261 actgctatga tgggtgcaat gaaagtaggc aacgatatta ctatcaaatt taaattgaat
     1321 tataccttaa aataaagtca tcaaattttt aacctgtata aaaaatatgt tatgaaaaat
     1381 tcaatcaacc aaaaattact ggctttagcg ctaactgctt tgtcagtatt gtcactgaat
     1441 gcacaaaaca atcaaaacga caaaatcaat tacgaccgcc cttacgacaa agccggtatt
     1501 aatgtatttg aaacaactaa aaaagatgca gttactttca ccggatttca attgaaaata
     1561 agtggtggat ttactcaaca gtatcaggca ttgtcacatg aaaatacagc agatgttatt
     1621 ttaaaagata acacacttac tgcaactgca ggtgactctg ttaatattaa tgccttatat
     1681 ccaatgacta atggatttaa cctagcaact gcgaatttaa ttttaactgc acagttagct
     1741 gatggtattg ccattaaatt agaaaattac atgtctgctc gtcaccacaa cgaattttgg
     1801 gtaaaaggtg gttatatcca aatggataaa ttaccatttt ttggtaaccc acaatggttt
     1861 gcagataatt tcaccgttaa agtaggacaa atggaagtaa actacggtga tgcacatttt
     1921 tacagaacag ataatgcgaa cggtatttac aacccatttg taggcgaaaa tatcatggat
     1981 gcatttgcaa ctgaaatttc aggtgaatta tattacaaac cagaaaacgg tattattgcg
     2041 atgttaggtt tatcatcagg attattaaat gcagacttat ctatttttaa agatcctgca
     2101 accggtgata ctattggaag aaaaccatct gtatatgcta aattgggata cgacaaacaa
     2161 ttatatgatg atttaagatt gagattaaca ggttctgttt atgcaaacgg tggaacttca
     2221 cgcaatacct tatatggtgg tgaccgcgca ggttcaagat attatttagc aggtgaagca
     2281 caatattaca tgtcaggaac aactttaacc gctgctactc caactaataa agctttctca
     2341 ggaagaattg acccaggttt ctccagcaat ttaatgagca ttatgattaa tccatttgtt
     2401 aaattccatg gtttagaagt atttggaact tatgaaatgt caagtggtag tgcaggttta
     2461 agcaacgtat tagattctgt tgatcgttca ttcagccaaa ttgcaggatc tgtagtttat
     2521 cgtttcttac ctgaagaaca atgttatgtt ggtgttcgat ataatacagt aactggtcgt
     2581 ttatatggat attctgacga tgatggtgat gtaaatgtaa atcgtttaca agttgccgca
     2641 ggttggttcc caactgaaaa tttattactc aaaattgagt acgtagatca aaaatatgat
     2701 aatttcaaaa ctaccgacgt tcgaaacgga cttgaattca aaggtgtgat ggttgaagca
     2761 tctgtaggat tctaagtagc ataatgaata ggttgaaaaa atcccgcgtc aattgatgcg
     2821 ggatttttgt ttgtagtatg atgtatttaa ttactgctta ataaatgtgt ttgtaaaatg
     2881 ttcattttct gcaactactt ctataacata catgccgctg ttgtaattag caacatctaa
     2941 caataaattt gattgttgaa tgttatgttt gagcattaat tttccggtaa tatcataaac
     3001 cgataattga caggcattta atggtcgggt aaatgtaatg tgtaatacag atgtggccgg
     3061 atttggttgc aatttcatta ttacatcaat ttcctgctcc tcaattccgg taatacaagg
     3121 cattaccaat aatgaacccg gagttcctgc agggcagctt tccatccaat tggatggttt
     3181 attcatgttt tcaccgatac tcacaatttg taaagcagtt ccaccaccat caggcgataa
     3241 aggataaggc gcaacatctt caaaacttac actttggaaa atttgaccat cctggtcgta
     3301 tataa
//
LOCUS       DAORKW010000427         3303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1602_length_3303_cov_3.810037, whole genome shotgun sequence.
ACCESSION   DAORKW010000427 DAORKW010000000
VERSION     DAORKW010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1602_length_3303_cov_3.810037"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="PLB46_13590"
     CDS             <1..1479
                     /locus_tag="PLB46_13590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77605.1"
                     /translation="TTLDLGFGENGIVRTDFGRLYEYGTGMVIQEDQKIVVVGRSCEE
                     KYTVCDAVAVRYNTDGSLDATFGEAGIVMLDFGNDENVYHVGLQSDGKIILCGYSEDK
                     NEHNDLLLIRLNTDGSLDTTFANNGVMINEDHFFTEAGKMVMQPDDKIVTLGYYFETS
                     GAQFMVNRYTADGFPDSTFGNAGVSYPYQSVNGVNYPHDIVMQEDGKILFCGEHGYYI
                     YKNACIVRILPNGSIDTTFSDDYNGVAEYNLGDGYCYNYALAVQSDGKILTAGRATYD
                     EPVGRQLFVIRTDEFGEYDNTFNQSGYLNFNYDGLGLNLTLIDITSDNKIIVAGTIDY
                     KGQRGVDCILARITEDGQLDNTFGYNGIVTQHLYTLNDNPYALALQADNKIVLSVNGD
                     YNPGNFDFNLIRFNPDATIPTAEDEEVTIFTNGIVNNELIITNTTNEGILSIYNMLGQ
                     LVFQQNACIFQTVINTTHFDSGVYFIHFQTGEQTQVLRCIKN"
     gene            1566..2717
                     /locus_tag="PLB46_13595"
     CDS             1566..2717
                     /locus_tag="PLB46_13595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77606.1"
                     /translation="MLKRIGILLTFGIYLIGINTIYAQAIPTFGPEIKVTISGLTFDA
                     MEPFISPDGNTLFFNSLNAGGNTNIYYALKENDSTFTFMGLVDGCYDPSPEHLDGVPA
                     MDTDNHFFWISLRNYPFVFENLFSGWYNDGAVTDISRVNGDFYIYEPGWLIMDATINY
                     EGNYLYFNNAYFNNCLLGMPCDARLGVAEKLNDTTFVKLWNSETLFANVNDTNYIVYA
                     PQVTQDGLELYFTRILNGTVNSEICVSTRANTDDAWSLPTVIYANLGFVPEAATITTD
                     KKIIYYHQKDETGIFHIYMRYREIADNIQDHELQPIVFSPNPVVDDLYLQSLLQQDEN
                     KVEIFNMAGKSVLQSSRCNPCSVWTLSSGMYVLSVTQNGVKKLTLFIKQ"
     gene            2865..>3303
                     /locus_tag="PLB46_13600"
     CDS             2865..>3303
                     /locus_tag="PLB46_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77607.1"
                     /translation="MKDHQLMYKLKAYSILAGSIVGSATALQADVIYTDIDPEVELPI
                     TGTEDYVTYTLSLNGDSSASFYFLNQVHNQYLYIDYEYVNSTLFNYFLVRGLYGGSIA
                     GVFTGGLLGNVASMFDSGATLGPDNNWVQPISSWYYGALFFVHD"
BASE COUNT         1034 a          525 c          664 g         1080 t
ORIGIN      
        1 accacacttg atttgggttt tggtgaaaat ggtattgtgc gcactgactt tggcaggtta
       61 tacgaatatg gcactggtat ggtaattcaa gaagaccaaa aaatagttgt ggtgggaaga
      121 agctgtgagg aaaaatatac tgtttgtgac gccgtagctg tcaggtataa tacagatgga
      181 agtttagatg caacttttgg cgaagccgga atagtaatgc tggattttgg caatgatgaa
      241 aatgtttatc atgtgggttt gcaaagtgat ggtaaaatta ttttatgtgg ctattcggag
      301 gataaaaacg agcacaacga tttactatta atcaggttaa acactgatgg tagtttagat
      361 acaacttttg ccaataatgg tgtcatgatt aacgaagacc atttttttac cgaagcagga
      421 aaaatggtta tgcagccgga tgataaaatt gtaaccctgg gatattattt tgaaacttcc
      481 ggcgctcaat ttatggttaa tcgttacacg gctgacggtt ttccagatag cactttcgga
      541 aatgcaggag tcagctatcc ctatcaaagt gtaaatggcg ttaattatcc ccatgatatt
      601 gtgatgcagg aggatgggaa aatactattt tgtggagaac atggttatta tatttataaa
      661 aatgcatgta ttgtacgtat attacctaat ggttcaattg acaccacttt tagtgatgat
      721 tacaatggtg tggctgaata caatttgggt gatggatatt gttacaacta tgcactggca
      781 gttcaaagtg atgggaaaat attaactgca ggcagggcga cttatgatga accggttggg
      841 cgacaattat ttgtgataag gacagatgaa tttggcgaat atgataatac ctttaatcaa
      901 tccggctact taaattttaa ctatgatgga cttggtttaa atttgacatt aatagatatt
      961 acttctgata ataaaattat tgttgcaggg acaattgact ataagggtca aagaggggtg
     1021 gactgtattt tagcaagaat taccgaagat ggacagttgg ataatacttt tggatataac
     1081 ggcattgtta cgcaacattt atatacttta aatgataacc catatgcact tgccctacaa
     1141 gcagataata aaatagtact tagtgtaaat ggagattata accccggcaa ttttgacttt
     1201 aatctaattc gttttaatcc tgatgccact attccaacgg ctgaagatga agaggttact
     1261 atttttacta atggaattgt taacaacgaa ttaattataa caaatacgac taacgagggt
     1321 atattgtcta tatataatat gcttgggcaa ttagtatttc aacaaaatgc ttgtattttc
     1381 caaacggtta taaatacaac tcattttgat tcaggggtgt attttattca cttccaaaca
     1441 ggtgaacaaa cacaagttct gcggtgtatt aaaaattagt gttgcacagt ctttttagcc
     1501 gaatcactat tcctagatga ttacttccac gcatttgatt atatttgaat aatcaatatg
     1561 catttatgtt gaaaaggatt ggcatcttat tgacattcgg tatctatttg attgggataa
     1621 ataccatcta cgctcaggct attcccacct ttggtccaga aattaaagtt accatttcag
     1681 gtttaacatt tgatgccatg gagcctttta tttctccgga cgggaacaca ctttttttta
     1741 attcgctgaa tgccggtggg aatacaaata tctactatgc gctaaaagag aatgattcta
     1801 ctttcacttt tatggggttg gtagatggtt gttatgaccc ttcacctgag cacttagatg
     1861 gagtgcctgc aatggatacc gacaatcatt ttttttggat atcactcaga aactatcctt
     1921 ttgtttttga aaatttattt tcaggttggt ataacgatgg agctgtaacg gatatttcga
     1981 gagtaaacgg cgatttttat atttatgaac cgggatggtt gattatggat gcaaccatta
     2041 attacgaagg caattatttg tattttaata atgcatattt taataattgt ttgttgggta
     2101 tgccttgtga tgctaggtta ggagttgccg aaaaattaaa cgacacaaca tttgtaaaat
     2161 tgtggaattc agaaacgctg tttgcgaatg taaatgatac caattacatc gtttacgcgc
     2221 cacaggttac tcaggacgga cttgaattgt atttcaccag aatattaaac ggaaccgtaa
     2281 attctgaaat atgcgtaagc accagagcca ataccgatga tgcctggagt ctaccgactg
     2341 ttatttatgc caatctcgga ttcgtccctg aagcagcaac tataacgacc gacaaaaaaa
     2401 taatttatta ccaccaaaaa gatgaaaccg gaatatttca tatttatatg cgttaccggg
     2461 agatcgctga taacatccag gatcatgagc tacaaccaat tgtgttttcg cccaatccgg
     2521 ttgtagatga tttgtatttg caaagcctac tgcagcagga tgaaaacaag gttgaaattt
     2581 ttaatatggc aggcaaatca gttctgcaat ccagccgctg caatccctgt tctgtttgga
     2641 ctttatccag tggtatgtat gttttatccg taacgcaaaa cggagtgaaa aagctaacct
     2701 tgttcataaa gcaataaaag caacctgcta tttcgaaaat ttatttttag ctttgataaa
     2761 cttatagtgg ttcctaagtt tagtagcgat gaaaattgct ccttggagta taccaaaaga
     2821 atgtttaatt ttattaactg aattcaacaa aacacaacat aaccatgaaa gaccatcaat
     2881 taatgtataa attgaaagcg tattcaattt tagccggcag tatcgttggc tcagcaacag
     2941 cacttcaagc agatgttatt tatacagata tagaccctga agtagaactc cctattactg
     3001 gaactgaaga ttatgttacc tacacgctta gtttgaatgg ggattcatct gcctcatttt
     3061 attttcttaa ccaagttcat aaccaatatt tatatattga ttatgaatat gtaaattcaa
     3121 cactgtttaa ttatttttta gtacgtggat tatatggcgg ttctattgcc ggtgttttta
     3181 ccggtggtct acttgggaat gtggccagta tgtttgattc aggcgctaca ttgggacctg
     3241 ataataattg ggtacaacca atttcatcat ggtattatgg tgcattattc ttcgtacatg
     3301 acc
//
LOCUS       DAORKW010000428         3296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1612_length_3296_cov_6.227090, whole genome shotgun sequence.
ACCESSION   DAORKW010000428 DAORKW010000000
VERSION     DAORKW010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1612_length_3296_cov_6.227090"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..587
                     /locus_tag="PLB46_13605"
     CDS             78..587
                     /locus_tag="PLB46_13605"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRF77608.1"
                     /translation="MNDLTSPGFGMAIYSKYPIEKTAVIKSHDFPLLTGNIVLKKGTV
                     SFIAATTSTPTNEKDYDKQLKQFKFIAEQANTIDGALIVLGDMNAVPWSEQVETLLKT
                     TRLKDSRKDLSATYPAQSPLQIPIDYIFHSQELSCEQFKTQGGTTSNHLGIIGYYNFK
                     TQGKNKSAK"
     gene            592..942
                     /locus_tag="PLB46_13610"
     CDS             592..942
                     /locus_tag="PLB46_13610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRF77609.1"
                     /translation="MLKKEITSFAAAFKGLFYLLKKERHFIIHIVIMLLVIIAGFYFH
                     LSQTEWVTLCLTFGLVICAEAFNTAIEKLCDIVHPEYHKSIGLIKDIAAAGVLFAAII
                     AIIIGCLIFIPKII"
     gene            944..1624
                     /locus_tag="PLB46_13615"
     CDS             944..1624
                     /locus_tag="PLB46_13615"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HRF77610.1"
                     /translation="MKIVHIQSQYKGKEKLQLILFTAFCFAMLIVRMIYTNSFEYRFM
                     GINLLLAWIPYLLVLRIIKLNTRGHKIQIGVLLFLWLIFFPNAPYMITDIYHLEEFDS
                     APKWFDLIMLLSFAWTGMMLAFISFRKLHRRFLRMKKTWVHLVTVFSIFFSCGIGIYL
                     GRYERWNSWEIITQPRVLTTEFLQLLNNQLAVAHMLSLSVVFGAFFTLIYLFVFTQTK
                     PVQISDKA"
     gene            complement(1603..2745)
                     /locus_tag="PLB46_13620"
     CDS             complement(1603..2745)
                     /locus_tag="PLB46_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRF77611.1"
                     /translation="MDISYILNILGEERGNYFNAVAPPIIQTSNFAFKTVAEFRNATK
                     DELHAHLYTRGNNPTTEIVRKKIAALEGTDDCLIFSSGAAAIAAAILHSVKSGDHVIC
                     VESPYSWTYYMLTQVFPLYGVTTTFVDGKSIENFENAIQPNTKLIFLESPNTFWLDLQ
                     DLAAICNLAKTKNIITAIDNSYCTPLYQQAHQLGVDLVIHTATKYLAGHSDVVSGVIC
                     ANQEHINSIFKTEFMAFGAIPSPNDTWLLLRGMRTLPMRLEKSAGNAEQLINWLAQHP
                     KVEKIIYPFHHSHPQFELAKKQMRRGSGLFAVLLKTQDKNKIEAFCNKLQRFLLAVSW
                     GGYESLVFPACIKEGSDYPVNFVRFYTGLEDADVLIEDINQALSEI"
     gene            complement(2887..3081)
                     /locus_tag="PLB46_13625"
     CDS             complement(2887..3081)
                     /locus_tag="PLB46_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRF77612.1"
                     /translation="MDFNPNLNFNEIIIRYALLIGIGILAGVFQNFFLIIPAMGVFLT
                     AILGWCPVKAMMSGSKNAAH"
BASE COUNT         1050 a          586 c          588 g         1072 t
ORIGIN      
        1 gggtgtctat gcaggaatta aaagcaaatg cgtttgacat agtggaccca ttgatgcgcg
       61 aataatatca atatgttttg aacgatttaa cttcaccggg ttttggtatg gcaatttatt
      121 caaaatatcc aattgaaaaa acggcagtaa ttaaatcaca cgatttccct ttgctcaccg
      181 gcaatattgt tttgaaaaaa ggaactgtaa gttttatagc cgcaacaaca agtacaccta
      241 ccaatgaaaa agattacgat aaacaattaa aacaatttaa atttattgca gaacaagcta
      301 atactattga tggtgcatta attgttttgg gagatatgaa tgcagtgccc tggagtgaac
      361 aggttgaaac gttattaaaa acaacacgat taaaagacag tcgcaaagat ttatctgcca
      421 cctatcctgc acagtcgcca cttcaaattc caatagatta tattttccat tcacaggaac
      481 tcagttgcga acaatttaaa acgcagggag gaacaaccag taaccactta ggtataatcg
      541 gttattataa ttttaaaaca caaggcaaaa ataaatccgc taaataatgt tatgctgaaa
      601 aaagagataa ccagctttgc tgctgctttt aagggtcttt tttatttgct aaaaaaagag
      661 cggcatttca ttattcatat cgtaataatg ttattggtta ttattgccgg gttttatttc
      721 cacctttccc aaaccgaatg ggtaacgctg tgtttaacat tcggtttagt tatttgtgct
      781 gaagcattta atactgctat agaaaaatta tgtgatattg tacatcctga atatcataaa
      841 tccattggtt taataaaaga catagcagct gcaggggttt tgtttgcagc gattatagcc
      901 atcataattg gttgtttaat ttttattccg aaaataattt aatatgaaaa ttgtgcatat
      961 ccaatcgcaa tataaaggca aagaaaaatt gcaacttatt ttatttacag cattttgttt
     1021 tgccatgctt attgttcgta tgatttacac caactcattc gaatatcgtt ttatgggcat
     1081 caatttgttg ttggcatgga taccttattt attggtttta cgcatcatta aactcaatac
     1141 cagagggcat aaaattcaaa ttggggtatt attgtttttg tggctgatat ttttccccaa
     1201 tgccccgtat atgattaccg acatttatca tttagaagaa tttgattctg caccaaaatg
     1261 gtttgactta atcatgttat tatcttttgc atggacaggc atgatgctgg cgtttatttc
     1321 gtttagaaaa ttacatcgtc gatttttacg catgaaaaaa acatgggtgc atcttgtcac
     1381 cgtatttagt atttttttca gctgtggtat aggtatttat ctcggccgct atgagcgatg
     1441 gaacagttgg gaaataatta cccagcctag agttttaacc accgaatttt tgcaattact
     1501 caacaatcag ttagcggttg cgcatatgct gtctctaagt gttgtattcg gcgcattttt
     1561 tacgttgata tacctttttg tttttacgca aacaaagccg gttcaaattt cagataaagc
     1621 ctgattaatg tcttcaatta atacatcagc gtcttctaat ccggtataaa aacgtacaaa
     1681 attaactggg taatcgctgc cttcttttat acatgcagga aataccagcg actcatatcc
     1741 accccaggaa actgctaata aaaaccgttg cagtttatta caaaatgctt caattttatt
     1801 tttatcctgt gtttttaata acacagcaaa taatccgcta ccacgtcgca tttgtttttt
     1861 agcgagttca aattgcgggt gactatgatg aaaagggtaa ataatttttt ctactttggg
     1921 atgttgtgct aaccaattaa tgagttgttc agcattacct gcactttttt ccaagcgcat
     1981 aggtaatgtg cgcatacctc gtaataataa ccaggtatcg tttggagatg gaatggcacc
     2041 aaatgccata aattctgttt taaaaatgct gttaatatgt tcctgattag cacatattac
     2101 accagacacc acatcactat gacctgctaa atattttgtt gccgtatgaa ttactaaatc
     2161 aacacctaat tgatgtgcct gctgatataa tggtgtgcaa taactattat cgattgcagt
     2221 aataatattt tttgtttttg ccaaattaca aatggcagct aaatcctgta aatcgagcca
     2281 aaatgtattc gggctttcta aaaaaatcag tttggtattt ggctgaatgg cattttcaaa
     2341 attctcgatt gattttccat ctacaaatgt agttgtaaca ccatacagcg gaaacacctg
     2401 tgtaagcata taatacgtcc agctataagg actctcaaca caaataacat gatcaccgga
     2461 tttaacacta tgcaaaatag cagcagcaat tgcagccgca ccgctcgaaa aaattaaaca
     2521 atcgtcggtg ccttccaaag cagcaatttt ttttcttaca atttcagtag ttggattatt
     2581 gcccctggta tataaatgcg catgtagttc atcttttgta gcattgcgaa attcagcaac
     2641 tgttttaaag gcaaaattgc tggtttgaat tatgggagga gcaacagcat taaaataatt
     2701 accgcgttct tcccctaaaa tgttaagaat ataggatatg tccatagtgc cgacaaatta
     2761 cggcaatttc gtacaaaata aaatggctcg tcggacgacg aaccattttt tcaggcatac
     2821 tcaaagaaaa gacaaccgta agacacgatt gcataaatgc aatgtgcttt tgttgcaaaa
     2881 cactctttag tgggcagcgt ttttgctgcc gctcatcatt gctttaacag ggcaccagcc
     2941 taaaattgct gttaagaata cacccattgc aggaataatc agaaagaaat tctgaaatac
     3001 accggcaagg ataccaatgc caattaataa agcatatctg ataataattt cgttaaagtt
     3061 tagattcgga ttaaagtcca taacatgtga ttttgttatg taaagatgat agctgtacca
     3121 ctgtgaaaag tatgacctac gtcagagtga acggtgattt tggtcactcg caaaaaagaa
     3181 attttgctcg tttagtcgcg tattatttgg ttgagcagtt cccgaactgc aaccggaggt
     3241 agtgttttag ccagttcaac ctgtttggta tattgttcca gcaactgctt ataatt
//
LOCUS       DAORKW010000429         3296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1614_length_3296_cov_4.920703, whole genome shotgun sequence.
ACCESSION   DAORKW010000429 DAORKW010000000
VERSION     DAORKW010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1614_length_3296_cov_4.920703"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1165
                     /locus_tag="PLB46_13630"
     CDS             212..1165
                     /locus_tag="PLB46_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77613.1"
                     /translation="MAVEHSFVYVLPYIIYIETELAIKSGEKKYLDKILQRHFKQVEE
                     VSNWVQTDLELLHAALKVVTINKSAVAQNSFKLEQYRSVMELPVLFADITALPVKQQR
                     NQLAILGNCYSMLNKNVEAVKIRKKIIDMYGPVNAIHPDSYKAYLSDLNNYLISAERA
                     GVKVNVMSHLDDLLAFTGKKIFEKKSFQYLKFRTFAYVFNYKLDYFLKHDKIKEATNI
                     YWQIADEFESWIAHISDYELFTFYKHFLIVEFVNNNYKAAADFCHSILQGSKNNVRMD
                     VVQFAELMLAVCLYEADERTYLKSYLNKTKRNAKQLNGRGE"
     gene            1196..1351
                     /locus_tag="PLB46_13635"
     CDS             1196..1351
                     /locus_tag="PLB46_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77614.1"
                     /translation="MLRLTDKKQKAPKLNGALKKLNALNEGKTKVDYFYDIRIWIQQK
                     LLQIKQR"
     gene            complement(1348..1884)
                     /gene="dcd"
                     /locus_tag="PLB46_13640"
     CDS             complement(1348..1884)
                     /gene="dcd"
                     /locus_tag="PLB46_13640"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRF77615.1"
                     /translation="MILTDKEIEKSIAEGTILIEPYRADCLGTNSYDVHLGRYLAVYK
                     NHILDAKKHNEIEEIIIPDEGFVIHPGILYLGVTEEYTETHAHVPFLEGKSSVGRLGI
                     DIHATAGKGDVGFCNTWTLEISCSQPVRIYAGMPIGQLIYFEVKGDITNYYNKKQNAK
                     YVNRTIRPVESMMFMNKF"
     gene            complement(1959..2897)
                     /locus_tag="PLB46_13645"
     CDS             complement(1959..2897)
                     /locus_tag="PLB46_13645"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRF77616.1"
                     /translation="MLLPSLIHIEKINHFTGHAAGIFSLGQGFAQHEILSGGGDGVIA
                     AWQVGKQENAKGLASIAGNVFALKLVPEMQLLVAGDLNGVLHIINLESKQEVARFTYE
                     GGAIFSIVQINSNTILAGAGNGNVYFIDLIQLKQIAVIEVSDKSIRAVCFIENRKELL
                     FACSDFNIYVYDADNFQLKNVLHGHDNSVFSIALNPSGTRLVSGSRDAQLRVWDVQNN
                     YTCMMTIPAHMFTINDIVFSPDGKLMATAGRDKHIKIWDAETFNLLKVIDREKFGGHI
                     NSVNKLFWSNWNNYLISCGDDKAVMVWEIVISEEGI"
     gene            complement(2987..>3296)
                     /locus_tag="PLB46_13650"
     CDS             complement(2987..>3296)
                     /locus_tag="PLB46_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77617.1"
                     /translation="EIRVNFNGGIKKFAQVKGIDYFHNQKQAHMFKFFQFIKSEQDII
                     HIEVPKDLKGKLVEITIQEKAPDPDTHLDKNDEEKMALKEAELKMLDKVRNQLNVWDI
                     "
BASE COUNT         1075 a          612 c          566 g         1043 t
ORIGIN      
        1 attaaagcaa caatgtaaaa acacaccgat aggtaacaac tgtaaatttt acaaaaatta
       61 cctaagtcag gcactggcaa catcgttgta cagaaatcaa tcctataacg attacaaatc
      121 tgtcattttt gaagggtgta gaatcgctga gatatatctt caaaaacaat tgtttgattt
      181 atgtgacaag caattaaaaa aaatgtatgc gatggcagtg gaacatagtt ttgtgtatgt
      241 tttaccgtat attatttaca tagaaacgga acttgccatt aagtcgggcg aaaaaaaata
      301 ccttgataaa atattgcaac gtcatttcaa gcaggtggag gaagttagta attgggtgca
      361 aaccgattta gaattgttgc atgctgcctt aaaggtggtg actattaata aatcggcagt
      421 agctcaaaat tcattcaaac ttgaacaata caggtctgtt atggaattac ctgttttgtt
      481 tgcagatatt actgcacttc cagtaaaaca acagcgaaac caattggcca tattaggtaa
      541 ttgttacagt atgctgaaca aaaacgttga agctgtcaaa ataagaaaaa aaataattga
      601 tatgtatggc cctgtaaatg ctattcatcc ggatagttat aaggcctatt taagcgattt
      661 aaataattat ttgatatcgg ctgagcgggc tggtgtaaag gtaaatgtga tgagtcattt
      721 agacgattta ttggcgttta caggcaaaaa aatatttgaa aaaaaatctt ttcaatattt
      781 aaagttccgc accttcgctt atgtatttaa ttataaattg gattattttc tgaaacacga
      841 taaaataaaa gaagcaacga atatatattg gcaaattgct gatgagtttg aatcatggat
      901 agcgcatatt tctgattatg aattatttac cttttacaaa cattttttaa tagttgagtt
      961 tgtaaataat aattataaag ctgctgctga tttttgtcat tctattttac aagggagcaa
     1021 aaacaacgtg cgaatggatg tagtgcagtt tgctgaactt atgcttgctg tttgtttata
     1081 tgaagctgat gagcgcacgt atttaaaatc ctatttaaat aaaaccaaac gcaacgcaaa
     1141 acaattgaat ggacgtggtg agtaggcaga agtggttttc aaattaattc accaattgtt
     1201 aaggcttacc gataaaaagc aaaaagcacc gaaattaaac ggtgctttaa aaaagttaaa
     1261 tgctttaaat gaaggcaaaa ctaaagtcga ttatttttat gatatccgca tttggataca
     1321 acaaaaatta cttcagatta agcagcgtta aaatttattc ataaacatca tactctcaac
     1381 aggacgaatg gttctgttga catatttagc attttgtttt ttgttgtaat aattcgtgat
     1441 atcacctttt acttcaaaat aaattaattg accaattggc atacctgcat aaatacgaac
     1501 aggttgtgaa caggaaattt ctaatgtcca tgtgttacaa aatccaacat ctcctttacc
     1561 ggcagttgca tggatatcaa ttcccaaacg accaacgctc gacttgcctt ctaaaaaagg
     1621 aacatgcgca tgcgtttcgg tatattcttc tgttacacct aagtataata tgccgggatg
     1681 aatcacaaat ccttcatcgg gaatgataat ctcctcaatt tcattatgtt ttttcgcatc
     1741 gaggatatgg tttttataaa ctgctaaata tcggcccaaa tgaacatcat acgaattggt
     1801 gcctaaacaa tctgcccgat acggttcaat caaaattgta ccttcagcta tcgatttttc
     1861 aatttctttg tcagttaata tcatgttgta aaaaatattt atttatcgaa atggttatgc
     1921 gtttttaatt ggcactacga tggcgaaact ccatatattt atatcccttc ctcgcttata
     1981 acaatttccc aaaccattac cgccttgtca tcgccgcacg aaattaggta gttattccag
     2041 ttgctccaaa ataatttatt caccgagtta atatgcccac caaatttttc cctgtcaatc
     2101 accttcagca gattaaaggt ctccgcatcc catattttga tgtgtttatc tcttccggca
     2161 gtcgccatta acttcccgtc aggactaaaa acgatatcat taatggtaaa catgtgtgcc
     2221 ggaatggtca tcatacaagt atagttgttt tgaacatccc aaaccctcaa ctgcgcatct
     2281 cttgaaccgc tcacaagcct tgtaccggaa gggtttaatg cgatactgaa aactgaatta
     2341 tcatgcccgt gtaagacgtt tttcagttga aagttatctg catcatacac gtaaatatta
     2401 aaatcactgc aggcaaaaag caactctttg cggttttcga taaaacaaac ggcacgtatc
     2461 gatttatcac tcacttcaat aaccgcgatt tgcttaagct gtatgagatc tatgaaatag
     2521 acattgccat tgccggctcc tgctaaaatg gtattgctat ttatctgcac aatactaaat
     2581 atagcaccac cttcataggt aaaacgggca acctcttgtt tggactccag attaataatg
     2641 tgtaacacac catttagatc tccggcaacc aataactgca tttcaggcac cagtttcaat
     2701 gcaaatacat ttcccgcaat acttgccaac cctttggcat tctcttgttt accgacctgc
     2761 caggctgcaa ttacaccatc gccaccacca ctcaatattt cgtgttgggc aaacccctgg
     2821 ccaagactaa aaatgcctgc agcatgacct gtaaagtggt tgattttttc aatatgaatt
     2881 aacgatggta aaagcatagt aataacacct tttagaaagg gctacaaaat tgcgaaaaca
     2941 aaataggaaa atgggagaga tgttaacagg ttggttaagg caagttttaa atgtcccaaa
     3001 catttagctg attgcgcact ttgtctaaca tctttaattc agcttctttt aacgccatct
     3061 tttcctcgtc atttttatcc agatgggtat ccgggtccgg agctttttcc tgaatggtta
     3121 tttccacaag tttgcctttg aggtccttag ggacttcgat gtggataata tcctgttcag
     3181 acttaataaa ctggaagaat ttgaacatgt gtgcttgttt ctgattgtga aaataatcaa
     3241 tccccttaac ctgtgcaaat ttttttatac cgccattaaa attaacccta atttcg
//
LOCUS       DAORKW010000430         3284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1624_length_3284_cov_4.702694, whole genome shotgun sequence.
ACCESSION   DAORKW010000430 DAORKW010000000
VERSION     DAORKW010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1624_length_3284_cov_4.702694"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLB46_13655"
     CDS             <1..248
                     /locus_tag="PLB46_13655"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HRF77618.1"
                     /translation="INRVVRRSVEFALANPAQTMEYVRENAQEMDEQVMQQHIALYVN
                     DFSVDLGEEGKAAVTALYEVAAEKKIIPGMHYPLFVA"
     gene            complement(257..862)
                     /locus_tag="PLB46_13660"
     CDS             complement(257..862)
                     /locus_tag="PLB46_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRF77619.1"
                     /translation="MPLHDDGYVKYTSIWTKTAVLNSAIDELVVCRNKLADLNLIGVY
                     PDGIGYGNISQRINQTAQFIISGTQTGQNKIALPEHFCKVTNVNIAENKLLCEGPVQA
                     SSEAMTHAAIYLADIHVKTVIHVHHQEMWQQLKNIVTTTDISIPYGTPEMANAMFSAL
                     KDIEKNKLPKIIITAGHQEGIFTFGNNLKDAFETLITFYQK"
     gene            996..1298
                     /locus_tag="PLB46_13665"
     CDS             996..1298
                     /locus_tag="PLB46_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77620.1"
                     /translation="MKNLLVAIAVLLVMVIGCNTTNEEVIADTQTAYFRDYRGLDGCI
                     WVIELADGTRLEPINLGDFDIVPADGKKIDIAYTPVSYASICMVGEMVEITAIIER"
     gene            complement(1299..2399)
                     /gene="mtnA"
                     /locus_tag="PLB46_13670"
     CDS             complement(1299..2399)
                     /gene="mtnA"
                     /locus_tag="PLB46_13670"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRF77621.1"
                     /translation="MNIKGKHYKTIWLHPNNENIVQIIDQRWLPHQLVIADIKSVDEM
                     CTAIKDMWVRGAPLIGAAAAWGMYLATVEVPASCARDFFFEECYGKLLKTRPTAVNLK
                     WALDEMLQSITGNDSIIESRWYGKTRAEWISADDIKVNEQIGNFGVQLIQEIAAKKKG
                     VVNILTHCNAGWLATVDRGTATAPIYKAKELGLPIHVYVSETRPRNQGAQLTAWELLE
                     NDIPHTLIVDNTAGHLMQNKLVDIVIVGTDRTSRTGDVANKIGTYLKALAANDNNIPF
                     YVAAPSSSIDFNMRNAFQELTIEERNPNEVKYVQGLENNQIKHVLIAPESTPAINYAF
                     DITPAKLITGLITENGICAANEIGITGLFPGK"
     gene            2441..>3284
                     /locus_tag="PLB46_13675"
     CDS             2441..>3284
                     /locus_tag="PLB46_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77622.1"
                     /translation="MSIFVHQPVFTQNKDIYLFIDSTTIIGKITENQIFTSPTNIDYT
                     LQGNSIYQGDTISRQQLLFLVNAKDILSKKAGLVYQNDSKTIQYITRNSIFYLGDHPL
                     DEENEDLLSIVIKNDSLYHVFSGIDGNQIGTIEGRFANQVQIVAAAHMYIKHYQLDDQ
                     VNVIMSSLTQNNNSGGLAYMRPANEYLPYYEWVWDGKVLKPAWGYRPDDEWSFDGKYI
                     KPLWSADPQTEWIWDGGTLKPFWDAGVEQQWIWKDNMLKPFWDSNPDLQWIFEEPYLR
                     PMWSF"
BASE COUNT          999 a          612 c          618 g         1055 t
ORIGIN      
        1 aaataaatcg tgtagtgaga cgaagtgttg aatttgccct cgctaatccc gcccaaacta
       61 tggaatatgt tcgcgaaaat gcgcaggaaa tggacgaaca ggtgatgcaa caacatatcg
      121 ccttatatgt aaatgatttt agtgttgatt tgggtgaaga aggcaaagca gctgttactg
      181 ccttatatga agttgctgca gaaaagaaaa taattcccgg tatgcattat ccgttgtttg
      241 tagcataatt ttattgctac ttttggtaaa acgttatcag cgtttcaaaa gcatctttca
      301 gattattacc aaatgtaaat atgccttcct gatgccccgc tgtaattata attttcggca
      361 atttattttt ttcgatatcc tttaacgctg aaaacatagc attagccatt tcaggtgttc
      421 catatggaat actgatatca gtagtcgtga caatgttttt taattgttgc cacatttcct
      481 gatgatgaac atgtataaca gttttgacat gaatatccgc caaataaata gcggcatgtg
      541 tcattgcttc agaagatgcc tgcaccggac cttcgcataa caatttattt tctgcaatat
      601 ttacattggt tactttacaa aaatgctccg gtagcgcaat tttattttga cctgtctgag
      661 taccggaaat aatgaattgt gctgtttggt taattcgctg actaatattg ccgtatccaa
      721 tcccatccgg gtaaacgcca attaaattta agtcagccag tttatttctg cacacaacca
      781 attcgtcaat tgctgaattg agcacagcag ttttggtcca tattgacgta tattttacat
      841 atccatcgtc gtgtaaaggc atatgcaaat gtaggggaat taaagtaatt cgcggatagt
      901 gaaggctttg caggctgctt aatcctgaca aaattattac atttttttcg gcactattta
      961 tgcattatct tcgttaaaga aacaaactta aagccatgaa gaatttgctt gtcgcaattg
     1021 cagtattact agttatggtt attggctgca acactacaaa tgaagaagtt atagcagaca
     1081 ctcaaacagc ctattttaga gattacaggg gtttagacgg ctgtatttgg gtaattgaat
     1141 tagcggatgg cacccggctt gaaccaataa atctggggga ttttgatata gttcctgctg
     1201 acggcaaaaa aattgatatt gcctatactc ctgtttccta cgctagtatt tgtatggttg
     1261 gagaaatggt agaaatcact gctattatag aacgctaatt attttcctgg gaacagtccg
     1321 gtaataccaa tttcatttgc ggcacaaatt ccattttcgg taattaatcc ggtaatcaac
     1381 tttgccggtg taatatcaaa agcataatta attgcaggag tagattcagg tgcgattaat
     1441 acatgtttaa tctgattatt ttccaaaccc tgtacatact taacctcatt tggatttctt
     1501 tcttcaatag ttaattcctg aaacgcatta cgcatattaa aatcaatact tgatgatggt
     1561 gctgctacat aaaaagggat attgttatca ttagcagcca gtgcttttaa ataggtacca
     1621 attttgttcg caacatcacc agtgcgtgaa gttctgtcgg taccaacaat aacgatatcc
     1681 actaatttat tttgcattaa atgacctgct gtattgtcta caatcaacgt atgcgggata
     1741 tcattttcca ataattccca ggcagttagt tgagcgccct gattacgcgg gcgggtttcg
     1801 ctaacataaa catgtattgg caaccctaat tcttttgctt tataaattgg tgctgttgct
     1861 gtgcctctgt ctaccgttgc taaccatcct gcattacaat gcgtcaaaat attgactact
     1921 cccttttttt tcgctgcaat ttcctgaatt aattggacgc caaaattgcc aatttgttca
     1981 ttcactttta tatcatcggc gcttatccat tcggcgcgtg ttttaccata ccatcgcgat
     2041 tcaataattg aatcattgcc agttatagat tgcagcattt catctaatgc ccattttaaa
     2101 ttaactgcag taggacgagt tttaagtaat ttcccataac attcttcaaa aaagaaatcg
     2161 cgcgcacaac tagccggaac ttccactgta gcaagataca taccccatgc tgcagcagcg
     2221 ccaattaaag gagcaccacg cacccacata tctttaatag ctgtacacat ttcatctacg
     2281 gattttatgt ctgcaataac taattgatgt ggcaaccaac gttggtcaat aatttgcaca
     2341 atattttcgt tatttggatg taaccaaatc gttttataat gtttaccttt aatattcata
     2401 ttatttgata aaagtgcgct ttgctatatt aataactatt gtgagcattt ttgttcacca
     2461 accggttttt actcaaaaca aggatatcta tttatttata gattcgacca caattatcgg
     2521 taaaattacg gaaaaccaaa tttttacatc tccaaccaat attgattata ccttacaagg
     2581 caatagtatt tatcagggag ataccattag tcgtcagcaa ctattatttc ttgttaatgc
     2641 aaaagacatt ttgagtaaaa aggcggggct ggtatatcaa aacgacagca aaacgataca
     2701 atacattacc agaaacagca tattttattt aggtgaccat ccgcttgatg aagaaaatga
     2761 agatttgttg tcgattgtaa ttaaaaacga ttcattatat catgttttca gtggtattga
     2821 tggcaatcaa atagggacca tagaaggtag atttgccaat caggttcaaa ttgtggcagc
     2881 agcccatatg tacatcaaac attaccagtt agatgatcag gttaatgtaa taatgtcgtc
     2941 tttaacacaa aataacaatt ccggcggatt ggcttatatg cgaccggcta atgaatattt
     3001 gccctattac gaatgggttt gggacggcaa agtgctgaaa ccggcttggg gatatcgccc
     3061 cgacgacgaa tggtcatttg atggaaaata cattaaaccc ttatggagtg ccgaccctca
     3121 aacggaatgg atttgggatg gaggaacgct aaaacccttt tgggacgcgg gtgttgagca
     3181 acaatggata tggaaggata atatgttaaa accgttttgg gatagtaacc ctgacctgca
     3241 atggattttt gaagaaccat acttaaggcc gatgtggagt tttg
//
LOCUS       DAORKW010000431         3283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1627_length_3283_cov_3.800496, whole genome shotgun sequence.
ACCESSION   DAORKW010000431 DAORKW010000000
VERSION     DAORKW010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1627_length_3283_cov_3.800496"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2678)
                     /locus_tag="PLB46_13680"
     CDS             complement(<1..2678)
                     /locus_tag="PLB46_13680"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF77623.1"
                     /translation="MRKILSVLCLVLAAFFVDAQTATLSGKVTDAGTGETLPGVNIIL
                     TGTTMGAISDFDGNYVVADIPPGEYNVQFTYIGYEKTVFTAQKFSAGEKKKLDIALKS
                     TVLTFDEAVVIVGEKPLVDVEDAKTGSTLTAKEIELQPVRQIQSVINMQTGVVQSSDG
                     VHIRGGRSYETGFYIDDVSARDPLSGTGFGIDIGTNSISKIDVTTGGAGVEFGNATAG
                     IVNTQTKSGDDKFEFNAGYKRDNFGFNKNAGSTWNQQVMEMGMGGPLAFKKKLTFYTT
                     LKTNFSDTFIPNSPNGEGAAAEQVVSSLYPNTQWSPYQDNRWAGMLKLDYKFDPKRRL
                     SFTYLKSITINQDYNMLRVTGNDVSFNPGYQFLFALQPDNANTYTHDTNLETIKWSQT
                     ISNTFSYQITGSRLYVKLRADANGAPWRPDVVNTEFDPESIVTYPATVFNPDDSIVFV
                     NPGPGLYNNGGIATLWHDHYVLEYTIRASANVYSKDTRNKLFFGTELKPQEYQWIDIT
                     RPWIGAPIQLADGSYSQSYRLGDISDVWKVNPLSGALFISDKLKYLGLIAEAGLRLEY
                     WFPGKFLDDAVADSTTTIAPAFRDAYYSDSYGLGERRFKMRLLPKFSASFPIKENQVL
                     FFNYGHSTVMPHPSYVYTGMDPYFADRSTLGFIGNPDLNPEVDIAYELGLKSSITSND
                     AINVTAFWKDKYDFITSSTILIEDITGREVARTIRINSDYARVRGIEIAYLKRIQKWF
                     SGSISLSYSIATGQSSSASEALGDILATGNSETSVETPLAWDSPIDAKTYAIFLLDNT
                     TGLFNKPWINKMTLEEKLAICKAWLEGKTLEISCKGENTWEVVSPITKTSDMYLNFSL
                     YNYKVKETPKPSIAYCYKMESSGHLIWSTKLIDTENT"
     gene            2936..>3283
                     /locus_tag="PLB46_13685"
     CDS             2936..>3283
                     /locus_tag="PLB46_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77624.1"
                     /translation="MRKLLLSFCTPIFILFLLGPNIDISAQAPAIEWQQSYGGTEADF
                     MNVALSTSDGGYFFAGDSYSSITGNKTENSNGATDFWVVKTDANGTIEWQNAIGGSSY
                     DVLYDAHQHPGGGY"
BASE COUNT          984 a          667 c          583 g         1049 t
ORIGIN      
        1 gtattttcag tatctataag ttttgtagac catataagat gtccagaaga ttccatttta
       61 taacaataag ctatagatgg tttaggtgtt tcttttactt tataattata aagggaaaag
      121 tttaaataca tatcagaagt tttagtaatt ggtgatacta cttcccatgt attttcacct
      181 ttacatgaaa tttctaaagt ttttccttca agccaagctt tacaaatagc taatttttct
      241 tctaatgtca ttttatttat ccatggttta ttgaataatc cggtagtatt atccaataaa
      301 aatatcgcgt aggttttagc atctatcgga ctgtcccagg ctaatggtgt ttccaccgat
      361 gtttctgaat ttcctgtcgc caaaatatct cctaaagcct cagatgctga tgaactctgg
      421 cctgtagcaa tactgtaact caaagaaata cttccgctaa accatttttg tatgcgtttt
      481 aaatatgcaa tttcgattcc tcgaacacgt gcataatcgg aattaattcg aatggtacgc
      541 gctacttctc tgcctgttat atcttcaatt aaaatagtac tcgatgtgat aaaatcatat
      601 ttatctttcc aaaatgcagt aacattaatg gcatcgttag aagtgatgga cgattttaag
      661 cctaattcat aagcaatatc tacttcagga tttaaatcag gattaccaat aaatcctaat
      721 gtacttctat cagcaaaata agggtccatt ccggtgtata cataactcgg atgcggcatt
      781 actgtgctgt gtccataatt aaaaaataat acctgatttt ctttaatcgg aaacgaagca
      841 gaaaattttg gtaataaacg cattttaaaa cggcgttctc ctagtccata actatcgctg
      901 taatatgcat ctctaaaagc aggtgcgatg gtagttgtgg agtcggctac agcatcatcc
      961 aaaaattttc ccgggaacca atattctaat cgcaaacctg cttcagcaat taatcccaag
     1021 tattttaatt tatcggaaat aaataatgct cccgataatg gatttacttt ccacacatca
     1081 ctaatatctc ccaaacgata tgattgtgaa tagctgccgt ctgctaattg tattggtgca
     1141 cctatccatg gtctggtaat atctatccac tgatattcct gtggttttaa ttctgtcccg
     1201 aaaaataatt tattgcgtgt gtcttttgaa taaacatttg cagatgcacg aatggtatat
     1261 tccaacacat aatgatcatg ccatagcgtt gcaataccgc cattattgta taaacccggt
     1321 cccggattta caaatacgat actatcatca ggattaaata cagttgcagg gtaagtaaca
     1381 atactttcag ggtcaaactc tgtattcacc acatcaggtc tccatggtgc accattagca
     1441 tctgcgcgta attttacata caatcgtgaa cctgttatct gataactaaa tgtattacta
     1501 atggtttgac tccacttgat tgtttcgaga ttggtatcat gggtataagt attggcatta
     1561 tccggttgta aagcgaataa aaactgataa cctggattaa aacttacatc attgcctgta
     1621 acgcgcaaca tattataatc ctgattaatg gtaatacttt ttagatacgt aaaactcagt
     1681 ctgcgcttag ggtcaaattt ataatccaat ttcaacatac ctgcccagcg gttatcttgg
     1741 tagggcgacc actgtgtatt gggataaagg gatgaaacta cctgctcagc agcggcgcct
     1801 tctccatttg gggagtttgg aataaacgta tcgctgaaat tcgttttaag tgttgtataa
     1861 aatgtcagtt tctttttaaa agctaacgga ccacccatgc ccatttccat tacctgctga
     1921 ttccaagtgc tgccggcatt tttattaaat ccgaaattat cgcgtttata tccggcgtta
     1981 aattcaaatt tatcatcacc tgatttcgtc tgtgtattga caatacctgc agtagcattc
     2041 ccaaattcca cacctgcacc accagtagtt acgtcaattt tggaaatact attagtcccg
     2101 atgtcaattc caaaccctgt tccactcaat gggtcgcgtg cggaaacatc gtcgatataa
     2161 aatccggttt cataacttct tcctcctcga atatgcaccc catcggaact ttgtacaaca
     2221 ccggtttgca tattaattac actctgaatt tgtcgcaccg gttgtaattc aatttctttg
     2281 gcagtaagag tggaacctgt tttggcatct tctacgtcaa ccaaaggttt ttcaccaaca
     2341 attaccaccg cttcatcaaa agtgagcact gtagatttta gggcaatatc caattttttc
     2401 ttttccccgg cgctgaattt ttgagcggta aaaactgttt tttcgtaacc gatgtaggta
     2461 aactgcacat tgtattcgcc gggaggaata tcagctacta cgtaattccc gtcaaaatcg
     2521 gaaatggcac ccatggtagt tccggtgaga ataatattta cgccgggaag ggtttcgccg
     2581 gtgccggcat cggtaacttt acctgataaa gttgcggttt gagcatcaac aaaaaatgct
     2641 gccaaaacca aacataaaac agataaaatt ttcctcattg gctcaaagtt gaataatttg
     2701 aatgaattag taaaaaaagc ttgtggaaaa caatgaaatt aacgtagaat taaaccggca
     2761 ggaacgcctg aaaaccaatt actttttagg catttgtcac aaaacaccca atattttatg
     2821 ataatttttg gtgataaggg tttggttggt tatttttagt actgaaacac cccatgtttt
     2881 ctgcattagc actttgtggg aaatgggtaa ttttttatta acaaaacaca caactatgcg
     2941 taaactgctc ctcagctttt gtactccaat ttttattctt ttcctattag gacctaatat
     3001 tgatatatcc gctcaagcac ctgcaatcga atggcagcaa tcctacggcg gaaccgaagc
     3061 cgattttatg aatgtagcgc tcagcaccag cgacggcgga tatttttttg ccggggattc
     3121 ttattcttct atcacaggta acaaaacgga aaactccaac ggcgcaaccg atttttgggt
     3181 agtaaaaacg gacgccaatg gcactattga atggcaaaat gctatcggcg gttcttctta
     3241 cgatgtgtta tacgatgccc accaacaccc cggtggtggg tac
//
LOCUS       DAORKW010000432         3276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1629_length_3276_cov_4.184725, whole genome shotgun sequence.
ACCESSION   DAORKW010000432 DAORKW010000000
VERSION     DAORKW010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1629_length_3276_cov_4.184725"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(527..1543)
                     /locus_tag="PLB46_13690"
     CDS             complement(527..1543)
                     /locus_tag="PLB46_13690"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRF77625.1"
                     /translation="MKKGKKKERQIMNSWWKNVFINNVFIDLKRKGKTAATVSQYELA
                     KHVKVLSITVRQVVKSFWLITLGVLCAGFGLRGFLLPSEFIDGGAVGISLLIAEVTHL
                     PLAVLLIVVNIPFVILGFNTIGKDFAIKTILGIIALACAVAFINYPTVTEDKLLVAIF
                     GGFFLGAGIGLAMRGGGVIDGTEVLAINLSKRIGGSVGDIIMVINIIIFSIAAYVLSL
                     ETAMYSILTYLAASKTVDFILDGIEEYTGVTIISHKSEEIRNMITDKLGRGVTIFNGK
                     RGFGTHGHNHRDIDIVYTVITRLEVSKLNAEIEKIDKSAFIVMSSIRDTKGGMIKKRV
                     HKHE"
     gene            complement(1670..2848)
                     /locus_tag="PLB46_13695"
     CDS             complement(1670..2848)
                     /locus_tag="PLB46_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRF77626.1"
                     /translation="MPNDLFEGVDYFQLDDLLTTEHKLIRDTVRQFVKQEISPIIEEY
                     AQKAETPKHLIKGLAEIGAFGPTIPVEYGGGGLDNIAYGLIMQEIERGDSGIRSLVSV
                     QGSLVMYPIYTFGSEEQRRKYLPKLGSGEMIGCFGLTEPDHGSDPGSMITNIKDKGDH
                     YLLNGAKMWISNSPFADIAVVWAKDDEGKIRGLIVERGMEGFSTPETHGKWSLRASAT
                     GELVFDNVRVPKENIFPEIKGLRGPLSCLSTARYGISWGALGAAMDCYDTALRYTQQR
                     KQFGKPIAGFQLQQKKLAEMITEITKAQLLVWRLGVLKNENKATPAQISMAKRNNVAM
                     ALAVARDARQMLGGMGITGEYSIMRHMMNLESVVTYEGTHDIHLLITGADITGIDAFK
                     "
     gene            3078..>3276
                     /locus_tag="PLB46_13700"
     CDS             3078..>3276
                     /locus_tag="PLB46_13700"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRF77627.1"
                     /translation="MATIVLEGMLFKSYIGIHDFEQAHGNRFEVTLTVESDAIISQND
                     QITETLDYGVLYNICNEVMAGR"
BASE COUNT         1051 a          700 c          511 g         1014 t
ORIGIN      
        1 agtgtaattt acaaaaaata gaaaaattta ataacttatt atcaacattt tttaagtgct
       61 tgtggttctg gtttctaaca gggaattgta attacctaaa ggttgttgtt tctcgcaaag
      121 acgcaaagca aacgctttct gtataatgca ccaatttaaa taaaaaacca attcaagcga
      181 gcaaaggtag tttgtgttaa aaagctttgc gcgcttgaaa acaatccata tcacacaatt
      241 tactatcaat tttcctttgc accttggcga gaaacaattg gttttccaac tctgcgggct
      301 caaaacaaac aacacttatt caaacaatct ccgcttctcc gcgtgaacca attggtttaa
      361 aagctttgcg cgcttgaaaa caatccatat cacacaaacc ataaacaatt taccttaggg
      421 tctttgcgag aaacaattgg tttacacgct ccgcggtctc aaaaaaaaca catcatacta
      481 acaaaccatc tccgcccctc cgcgtgaacc agcaccacac gaactgttat tcatgcttgt
      541 gcacccgttt tttaatcata ccacccttag tatctcgaat actgctcatg acaataaatg
      601 cagatttatc aattttttcg atttcagcat tgagtttgct tacttccaaa cgggttatta
      661 ccgtgtaaac aatatcaata tcgcgatgat tatgcccgtg cgtaccgaag cctcttttac
      721 cattaaatat agtaacacct ctgcctaatt tatcagtaat catattcctt atttcttccg
      781 atttgtgaga aataattgtc accccagtat attcttcaat accatctaaa ataaaatcaa
      841 cggttttaga tgcagccaga taagttaaaa tcgaatacat agcagtctcc aaggataaca
      901 catacgcagc aatagaaaat attatgatat taattaccat aataatatca cctacacttc
      961 ctccaatacg tttgcttaaa ttaattgcca atacttccgt tccatcaata actcccccac
     1021 cacgcatggc taagccaatt cctgcgccta gaaaaaatcc accaaaaatg gctaccagta
     1081 atttatcttc cgtgacagtc ggataattaa taaatgccac agcacaagcg agcgcaataa
     1141 tgcctaaaat tgtttttatg gcaaaatctt ttccaatcgt attaaatcct aaaataacaa
     1201 atggaatatt tactacaatt aataaaactg ctagtggtaa atgagttact tctgcaatta
     1261 ataatgaaat accaactgca cctccatcaa taaattcgct gggtaataaa aacccgcgca
     1321 agccaaaccc ggcgcataat acgcctaaag tgatgagcca gaaacttttt acaacttgcc
     1381 taacggtaat agagagcact ttaacgtgtt tcgctaattc atattgcgaa actgttgctg
     1441 ctgttttgcc ttttcttttt aaatctatga atacattatt aataaataca ttcttccacc
     1501 aagaattcat tatttgtcgc tccttttttt tgcctttttt caaaaataat aaaaaaagtc
     1561 tgcatttcct aattgaaaaa atgggcgaaa cattatcaat aattccttca ccacaaaaaa
     1621 aaaactgcaa tacgaatagt attgcagttt taaaagtgat taaatatttt tatttaaatg
     1681 catcaattcc ggtaatatca gctccggtaa ttaataaatg aatatcatgt gttccttcat
     1741 aggttactac actttccaaa ttcatcatat gacgcataat tgaatattcg ccggtgatac
     1801 ccataccacc taacatttga cgagcatcgc gagctacagc taatgccatg gccacgttgt
     1861 tacgttttgc cattgaaatt tgagcagggg tagctttatt ttcatttttc aatacaccta
     1921 atcgccaaac caataattgt gctttggtaa tttcggtaat catttctgcc agtttctttt
     1981 gttgtaactg gaaccctgca attggtttac caaattgttt acgttgttgt gtatatctta
     2041 aagcagtatc gtaacaatcc attgcagctc ccagagcacc ccagctaata ccgtaacgtg
     2101 cagttgacaa acaacttaat ggtccgcgca agcctttaat ttcagggaaa atattttctt
     2161 taggtactct cacgttatca aacacaagtt cacctgttgc acttgcacgt aaactccatt
     2221 tgccgtgggt ttcaggggtt gaaaatcctt ccattccgcg ttcaacgatg aggccgcgaa
     2281 ttttaccttc atcatctttt gcccaaacaa ctgcgatatc agcaaaaggc gaattactaa
     2341 tccacatttt agcaccattt aagaggtaat ggtcgccttt atctttaata ttggtaatca
     2401 tgctaccagg gtcagaacca tgatccggct ccgttaatcc gaagcaacca atcatttctc
     2461 cgcttcccaa ttttggtaaa tattttctgc gttgttcttc gcttccaaaa gtataaatag
     2521 gatacatcac caaacttccc tgcacgctca ctagcgacct gattccgcta tcgccgcgtt
     2581 caatttcctg cataattaaa ccataagcga tgttgtcgag gccacctccg ccgtattcaa
     2641 cagggatagt tggtccaaaa gcgccaattt ctgcgagacc ttttataagg tgtttcggag
     2701 tttctgcttt ttgagcatat tcctcaataa ttggtgaaat ttcctgtttt acaaactggc
     2761 gaacggtatc acgaataagc ttatgttctg tagtaagtaa gtcatctaac tgaaaatagt
     2821 ctactccttc aaaaagatcg tttggcatat aattcggttt aaattttgcg caaagttaag
     2881 gcattttacc tggataaact gataaacact aaacgttaat aacctaaaat tcccggttaa
     2941 aatgtgcatc aaggtggcaa taccaaaatt tgatacaatg cctgcaacca ttggactatc
     3001 tgcctttcga caaattgtta cctgtcaatt acactattaa accttgttta atctgtaatt
     3061 ttgtgtaaaa aaaagagatg gcaacaattg ttttggaggg catgttattt aagagttata
     3121 tcggaataca cgatttcgag caagcacatg gcaataggtt tgaggttacc ttaacagttg
     3181 aaagtgatgc catcatcagc caaaatgacc aaataacaga aacgctggat tatggtgttt
     3241 tatataatat ttgcaatgaa gtaatggcag gcagat
//
LOCUS       DAORKW010000433         3273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1631_length_3273_cov_3.896520, whole genome shotgun sequence.
ACCESSION   DAORKW010000433 DAORKW010000000
VERSION     DAORKW010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1631_length_3273_cov_3.896520"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLB46_13705"
     CDS             <1..885
                     /locus_tag="PLB46_13705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77628.1"
                     /translation="TVHIKMSGSKMMFDNRYVAEYYDYVYAVLDYGNKLNDAVTADEM
                     YIKTAGTYNMPNGLMDLEITGMTTPGFVRVEHYWVEADGFKNIHPGLHTSNQRYWRVI
                     SNLADDVELEAKFLYNGQATLSGGYLDDKFITNSDDSLVLLYRASPNDDWTIYANYTL
                     NTWGSTTDKRGAMELTKLKSGEYCFGIYDSEIPDKPTEQNLSCPHSDPVINNSPAFIQ
                     IIPNPADSQVIVTMDQLLIGNSIKMVDMAGKIVFTTTILTQQTSIPTTGLPAGTYQLY
                     VENPRSERIAAEKVVIIH"
     gene            906..1790
                     /locus_tag="PLB46_13710"
     CDS             906..1790
                     /locus_tag="PLB46_13710"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRF77629.1"
                     /translation="MKNITVIGAGTMGNGIAHVFAMKGFKVNLVDVSEPALQKAIQTI
                     SGNLDRMVAKEKITAADKENTLANLSTFTNFEHSANADLVVEAATERVDLKLKIFTEL
                     DQICNPDTILASNTSSISITKIAAATKRPDKVIGMHFMNPVPVMKLIEVIRGYATSDT
                     TTATIMSLSQQLDKIPVEVNDYPGFVANRILMPMINEAIYTLYEGVAGVNEIDTVMKL
                     GMAHPMGPLQLADFIGLDVCLSILNVLYDGFGNQKYAPCPLLVNMVTAGYLGVKSGEG
                     FYSYAAGSKELVVAPRFK"
     gene            1822..2751
                     /locus_tag="PLB46_13715"
     CDS             1822..2751
                     /locus_tag="PLB46_13715"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HRF77630.1"
                     /translation="MTPVFAKAYGPAEVHFQLEDCDARVEPRKVLMCTPEFYTIVDVK
                     NVHMQNQKGNVNKEKANIQWDAIRNTYLAFKNQKLIDEVYVIDGVDSLEDMVFVANQS
                     FPWVMWNGEKIALMSRMRFENRQNEIPYIEDFYKDHGYKIVELQSKYHFEGMGDMVAH
                     PGKRLLYGGYGFRSEKQVYDEISRTLDVPIITLELINENHYHLDTCFLPLNHTEVLIA
                     PDAFTPESIAIIRRMFEGVYEIPDTEAIEGFACNAHIIYGTNRATSGAIIQKNNPVAL
                     NIIQQSANVVAEVETSEYIKSGGSVFCLKMMVY"
     gene            2803..>3273
                     /gene="bioD"
                     /locus_tag="PLB46_13720"
     CDS             2803..>3273
                     /gene="bioD"
                     /locus_tag="PLB46_13720"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HRF77631.1"
                     /translation="MKKLFITGIGTDIGKTIVSAVVVEALQADYWKPVQSGGLDYTDT
                     ERVRNLISNTKSQFHPENYLFKFPVSPHLAAAMSETAILTETLIPPITKNHLVIEGAG
                     GLMVPLNGNFLMLDLIKQFDATVILVSKHYLGSINHTLLTNSNAFYDFSASNANG"
BASE COUNT         1092 a          547 c          644 g          990 t
ORIGIN      
        1 accgtacaca taaaaatgag tggtagtaag atgatgttcg acaatcgata tgtagcagaa
       61 tattatgact atgtatatgc tgttttggat tatggaaata aattaaatga cgcagttaca
      121 gctgatgaaa tgtatataaa aacagcaggc acttataata tgcccaatgg attaatggat
      181 ttagaaatta caggcatgac aacaccgggt tttgtgcgcg tagaacatta ttgggtagaa
      241 gctgatggct ttaaaaacat ccatccaggg ttgcatacca gcaatcaaag atattggaga
      301 gtaatatcaa atttagcaga tgatgttgaa ttagaagcca aatttttata taatggtcag
      361 gcgacacttt cgggtggtta tttagatgac aaattcatca ccaatagcga tgatagttta
      421 gtattattat atcgcgcaag tccgaacgat gattggacaa tttatgctaa ttatactttg
      481 aacacctggg gaagcacaac tgacaaaaga ggagcaatgg aattgacaaa acttaaaagt
      541 ggtgaatatt gttttggcat ttatgacagc gaaattcctg acaaaccaac tgaacaaaat
      601 ttgagttgcc ctcatagtga tccggttata aataattcgc ctgcattcat acaaattatt
      661 cctaatcctg cagatagcca agttatagtc acaatggacc aattacttat tggtaattca
      721 ataaagatgg ttgatatggc aggtaaaatt gtatttacaa caacaatttt gacacaacaa
      781 accagcattc cgacaacagg ccttccggca ggaacttatc aactttatgt tgaaaaccca
      841 cgttctgagc ggattgcggc cgaaaaggtg gtaattatac attaatattg cgcaaaaatt
      901 gtgcaatgaa aaacattacc gttataggtg caggtaccat gggaaatggt atagcgcatg
      961 tgtttgccat gaaaggcttc aaagtaaatc ttgttgatgt ttctgagcct gcactgcaaa
     1021 aagccataca aaccatatcc ggcaacttag accgtatggt ggcaaaagag aaaattactg
     1081 cagcagataa agaaaataca cttgccaatt taagtacgtt tactaatttt gaacattctg
     1141 caaatgccga ccttgttgta gaggccgcaa ctgaacgagt agatttaaaa ttgaaaattt
     1201 ttactgaact cgatcaaatt tgtaatcccg ataccatttt agcgagcaac acttcttcta
     1261 tttccattac caaaattgca gcagcaacca aacgacctga taaagttatt ggtatgcatt
     1321 ttatgaatcc tgttccggta atgaaattaa ttgaagtaat tagagggtat gctaccagcg
     1381 atactacaac tgctactatc atgtcgctca gtcagcaact cgataaaatt cctgttgaag
     1441 taaatgatta ccccggtttt gttgccaaca gaatattaat gccaatgatt aacgaagcca
     1501 tttataccct ttacgaaggt gttgccggtg taaatgaaat tgatacagta atgaaactcg
     1561 gtatggcaca tccaatgggt ccgctgcaat tagctgattt tatcggatta gatgtttgcc
     1621 tcagtatttt aaatgtatta tacgatggat ttggcaatca gaaatatgca ccatgtccgt
     1681 tgttagttaa tatggttact gcaggatatc tcggtgttaa atccggagaa ggattttata
     1741 gttatgcagc aggcagcaag gaattagttg ttgcacctcg ttttaaataa ttttttttca
     1801 tcgattacta atatttttta catgacacct gtttttgcta aagcgtatgg tcctgccgaa
     1861 gtgcatttcc agctagaaga ttgtgatgcg cgagttgaac cacgaaaagt tttgatgtgc
     1921 acgccggaat tttacaccat tgtagatgta aaaaatgtac acatgcaaaa tcagaagggc
     1981 aacgtaaata aagaaaaagc caatattcag tgggatgcta ttcgcaatac ttacctcgca
     2041 tttaaaaatc aaaaattaat tgatgaagta tatgtaattg atggcgtgga tagtttggaa
     2101 gatatggtat ttgttgccaa tcagagtttt ccatgggtaa tgtggaatgg tgaaaaaatt
     2161 gctttgatga gtcgtatgcg ttttgaaaac cgtcaaaatg aaattcctta tatagaagat
     2221 ttttataaag accatggtta caaaattgta gaattacaaa gtaaatatca ttttgaaggt
     2281 atgggcgata tggtagcaca tccgggaaaa agattgttat atggtggcta cggtttccgc
     2341 agcgaaaaac aagtgtatga tgaaatttcc agaacacttg atgtgccgat tattacctta
     2401 gaattaatca acgaaaatca ttaccatctc gacacctgtt ttttaccgtt aaatcacacg
     2461 gaagtattaa ttgctcccga tgcatttaca ccggaatcga tagctattat acgacgcatg
     2521 tttgaaggcg tatacgaaat tcctgataca gaagcgattg aaggttttgc ctgtaatgcc
     2581 catatcattt acggaactaa tcgtgccact tcaggtgcaa ttatccaaaa aaacaatccg
     2641 gtagcgctca atatcattca gcaaagtgcc aatgttgtgg ctgaggtaga aaccagtgag
     2701 tatattaaaa gcggtggttc tgtattttgt ttaaaaatga tggtatatta gcaggcgact
     2761 tagttgaacc actgttaact ttttgttcaa ctacatgaac acatgaagaa attatttatt
     2821 accggaattg gtactgatat aggtaaaact attgtttcgg cagttgttgt agaagcatta
     2881 caagccgatt attggaaacc tgttcaaagt ggtggtcttg attataccga cactgagcgt
     2941 gtacgcaatt taatatctaa cacaaaaagt cagtttcatc ccgaaaatta tttattcaaa
     3001 ttccctgttt caccacatct tgccgccgca atgagtgaaa ctgctatttt aactgaaaca
     3061 ttaattccgc caattacaaa aaatcattta gtaatagaag gtgcaggagg attaatggta
     3121 ccattaaatg gcaacttttt gatgctggat ttaattaaac aattcgatgc aacggtaatt
     3181 ttagtctcca aacattattt gggtagcata aatcatacgt tattaacaaa ttctaatgcc
     3241 ttctatgatt tttctgcaag taatgcaaat ggt
//
LOCUS       DAORKW010000434         3261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1648_length_3261_cov_3.571117, whole genome shotgun sequence.
ACCESSION   DAORKW010000434 DAORKW010000000
VERSION     DAORKW010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1648_length_3261_cov_3.571117"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PLB46_13725"
     CDS             <1..1009
                     /locus_tag="PLB46_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77632.1"
                     /translation="NMTPTKEVWLSVSLHVYEQAGPKILTTTSKKFQFGKAAIININK
                     TNLETYGPISTVYHQRSFKNELAKQGGIFPSGDYKVEVICNYEGNGFSEPLGKYVYNI
                     DAELVMPIQLVSVFNNDTIEDAYPMFNWTPPYPLPTGNLTYEIVLTEIKPLETPIAAV
                     KRNQPLIRNQQVNQNALIYTTVEPKLIAGKEYAWLVNAYTERGDFYSGSETWRFIYNP
                     EEDTIDYPDQFYLMSSVIPNTYVTIFDNILPVKFVDDYEVVDTLSYLHLYDDNFALIA
                     TEEQIPIAHTLGTNYSYVRFCEDDFPIPNGRYVLEIGGVNKQKYYIRFQNNSLVGACG
                     E"
     gene            1011..1559
                     /locus_tag="PLB46_13730"
     CDS             1011..1559
                     /locus_tag="PLB46_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77633.1"
                     /translation="MKNTFIFYTCIVLSSCSTKLDSLNYNELKSFVTSDEAALRESKN
                     INGQTVSYYYLPTDYLIANEVKGTSIDKNMYDSLYQLFAQYHYFEIQIEGTEELLRNT
                     GFVEKGMIFIETNKENVYPAFSVIEPGYGILPFNKVLVAFPNNFSNDSKVYLKVNVGN
                     STLYKFNVAFSKIKKYPQLLIQ"
     gene            1570..>3261
                     /locus_tag="PLB46_13735"
     CDS             1570..>3261
                     /locus_tag="PLB46_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77634.1"
                     /translation="MLNKLRNSRIVKCIVMLTGFFVVFDVFTITKLFALTSGPSQPEF
                     TGFSNVTSNNLVNPFTGDFSYSIPMLEIGGIPLGLTYNAGVGMDEEASWVGLGWSFNP
                     GSISRDVRGLPDDFNGENIISNINLKPNTTYGVTLGAELELFGLTNKKKSSDTIGSFN
                     LGANWSFIYNNYTGFDVQSSISPSISSAISSKLPLSASLSTSIGSTSDGSVSDISISP
                     QITFTRNATRAEENGKNNTSLGLTFNSRKGFQGLTLSSNYSETRTSAIDVSFKYQATS
                     GNMSRSSTVLPPNRTYKPSNTNDFTNNSFTFNGKLGITAFGLDGTASITGFMSNQKLE
                     ESQKKSSNPAFGYLYLREPGNENLLDFNREQDGSLEPTTKNLAPGFLTYDVYSVSGPG
                     MSGVFRPYRNEVGYISDPVSEFHSTSGSAGLEFAGGWAVSPGVDVSVTKVTSNSGAWT
                     EQNAVDDYTHYNSAYEINKFRERSYFAFVGDNGAENNEEVVNLFGKKGVTSFELAGDE
                     KNASLKSNFLNVENSNKYFDGPIIRENRVARKNEITYLTKKQISDYKPYRQPYLSS"
BASE COUNT         1094 a          523 c          602 g         1042 t
ORIGIN      
        1 aaatatgaca cctactaaag aagtttggtt gagtgttagt ctgcacgtat acgaacaagc
       61 agggcctaaa atactcacta ctacttctaa aaaatttcaa tttggtaaag ctgctatcat
      121 aaatattaat aaaaccaatt tagaaaccta tggtccaatt agcacagtat accaccagcg
      181 aagtttcaaa aatgaattag cgaagcaagg cggaattttc ccatccggtg attacaaagt
      241 tgaagtgatt tgtaattatg aaggaaatgg attttctgag cctttgggga aatatgtata
      301 taatatcgat gctgagttgg ttatgcctat acaactggtg agtgtgttta ataacgatac
      361 aattgaggat gcatatccga tgtttaattg gacacctcca tacccattac caaccggtaa
      421 tttgacatat gaaattgtat tgactgaaat aaaaccatta gaaacaccta ttgcggcagt
      481 taagagaaat cagcctctga ttagaaacca acaagtaaat caaaacgcat tgatttatac
      541 cactgttgaa ccgaaactaa ttgcaggtaa ggaatatgca tggctggtca atgcctatac
      601 agaaagaggt gatttttatt ccggaagcga aacatggcgg tttatatata atccggagga
      661 agacactatc gattatccag accaatttta tttgatgtca tctgtcatac caaataccta
      721 tgttaccatt tttgacaaca tactgccagt caagtttgtg gatgattacg aggttgtaga
      781 tacactaagt tatttacatt tatatgatga caattttgca ttgattgcta ctgaagagca
      841 aattccaatt gctcacacct taggtactaa ttattcatat gttcgttttt gtgaagatga
      901 tttccctatc ccaaatggac gttacgttct agaaataggg ggcgtaaata aacaaaaata
      961 ttatataaga tttcaaaata acagcctagt gggggcctgt ggtgaataaa atgaaaaaca
     1021 cttttatttt ttatacttgt atagtgctct caagttgttc aactaaatta gatagtttga
     1081 attacaatga gttaaaatca tttgttacat cagatgaagc agcgcttcgc gaatctaaaa
     1141 atattaacgg gcaaactgtc agctattatt atttgccaac agattattta attgcgaatg
     1201 aagttaaagg aacaagtata gataaaaata tgtatgattc gttatatcag ctatttgcgc
     1261 aatatcatta ctttgagatt caaatagaag gtactgaaga gttattgaga aacactggtt
     1321 ttgttgaaaa aggtatgatc tttattgaaa cgaataaaga aaatgtttac ccagcgtttt
     1381 ctgttattga gccgggatat ggtatactgc cctttaataa agtattagtt gcatttccaa
     1441 ataatttttc aaatgattcg aaagtttatt taaaagttaa tgttggaaat tctacacttt
     1501 ataaattcaa cgttgctttt tctaaaatta aaaaataccc tcaattatta atacaataaa
     1561 ctaattgtta tgttaaacaa acttcgtaat tcaaggattg taaagtgcat cgtaatgctt
     1621 accggatttt tcgttgtatt cgatgtattt accataacta aattatttgc attaacatca
     1681 ggaccttcgc aaccagaatt tactggattt tctaatgtaa cttcaaataa tttggtaaat
     1741 cccttcaccg gagatttttc ctattctatt cctatgctag aaattggtgg tattccttta
     1801 ggcttaacat acaacgccgg cgttggaatg gatgaagaag ccagttgggt tggtttaggt
     1861 tggagtttta atccaggatc tatttcgcgt gacgttaggg ggctacctga cgattttaat
     1921 ggagaaaata ttattagcaa tataaattta aaaccgaata caacttatgg tgttaccctt
     1981 ggtgctgaac ttgaattatt tgggcttact aataaaaaga aaagtagtga tacaattggc
     2041 agttttaact tgggtgcaaa ttggtctttt atatataata attacactgg ttttgatgtg
     2101 caatcaagta tttccccttc tatttctagt gcaatttcca gcaaattacc tttaagtgct
     2161 tctttatcca cttcaattgg aagtacgtct gatggcagtg tttccgatat ttcaattagc
     2221 ccgcaaatca catttactag gaatgcaact cgggcagaag aaaatggtaa aaataatacc
     2281 agtctaggtt taacttttaa ttcaaggaaa gggtttcaag gtttaacgtt aagcagcaat
     2341 tattccgaaa cacgaacttc tgctattgat gtatctttta aataccaagc tactagcgga
     2401 aatatgtcgc gttcctcaac tgtgttgccg cctaatcgca cctataaacc aagcaatacc
     2461 aacgatttta caaataactc attcacattt aacggtaaat tagggattac ggcatttggt
     2521 ttagatggta cggccagtat tactggattc atgtctaatc aaaaacttga ggaatcgcaa
     2581 aaaaaatcta gtaatccagc ctttggttat ttatacttga gggaaccggg taatgaaaat
     2641 ttattggatt ttaaccgtga gcaggacggt tcacttgaac caacaacaaa aaatttggca
     2701 ccgggatttc tgacttatga tgtttattca gtaagtggtc cgggaatgtc aggtgtgttt
     2761 cgaccatacc gaaatgaagt cggatatatc tcagatcctg taagcgaatt tcacagcaca
     2821 tccggatccg caggactgga atttgccgga ggttgggcag ttagccccgg ggtagacgtt
     2881 agtgttacta aagtaacatc taattctggc gcatggacag aacaaaatgc agttgatgat
     2941 tatacgcact ataactctgc atacgaaatc aataagttta gagaacgttc ttattttgct
     3001 tttgttggcg ataatggtgc tgaaaataat gaagaagtag ttaacttatt tggtaaaaaa
     3061 ggcgttacct ctttcgaact ggctggtgat gaaaaaaatg cttctttaaa aagtaatttt
     3121 ttaaatgttg agaatagtaa caaatatttt gatggcccta ttataagaga aaatcgagtt
     3181 gcaagaaaaa acgaaattac ttatttaact aaaaaacaaa tatcagatta taaaccttac
     3241 agacaaccat acctgtcttc t
//
LOCUS       DAORKW010000435         3253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1652_length_3253_cov_5.060663, whole genome shotgun sequence.
ACCESSION   DAORKW010000435 DAORKW010000000
VERSION     DAORKW010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1652_length_3253_cov_5.060663"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..2477)
                     /locus_tag="PLB46_13740"
     CDS             complement(321..2477)
                     /locus_tag="PLB46_13740"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRF77635.1"
                     /translation="MKKKLFSIILIIFTFIQVKADEGMWLPILLEQLNIEDMQARGFK
                     LTAEDIYSINKTSMKDAVVLFNGGCTGEIISNEGLMLTNHHCGFSSINSLSTLENNYL
                     RDGFWAKNKEQEIPAPGLTATFIISMFDVTDSIIPYLNENMDEATRNMTIKQLSAKLE
                     AQFKKGTHYDATVRAFYYGNEFYLFLTETFKDLRLVGAPPSSVGNFGGETDNWMWPRH
                     TGDFSIFRIYAGKDNLPAAYDPNNKPFKPRYSFPISLKGVEENDFTMVYGFPGRTQEY
                     LTSYAVDLTINTTNPNRIECRDQRLAIMNKYMSGNDTVTLMYASKAKGLANAYKKWKG
                     EMIGLKLNDAVTKKQTYEAKFQQWANTHEGKDYRNLLKDMEAAYTEYKAYSELVDYSS
                     EAFFAVELLNFASGYKKIAALTASDTISPEYYQQEAANFYTNSLNFYSKYYVKIDKEV
                     FATMMKLYAENVAPALQSDYFKAEQAKYQNNYNAWADALFSKSVFVSAEKLQKAMSNF
                     NSKKAKKLLNDPAYRLYAEVANMYDTQIKSGINPLNEKIGIYMREYMKAQRLMQPDRD
                     FYPDANSTLRVTYGNVKGYTAKDAVYYTYQTTDEGIEQKYIAGDEDFDMPASLIALFE
                     KNNFGRYANKEGELPIAFIASNHTTGGNSGSPVINANGELIGTNYDRVWEGTMSDIMY
                     DVNRCRNISLDIRYTLFIIDKYAGATNIIQELNIVE"
     gene            2752..>3253
                     /locus_tag="PLB46_13745"
     CDS             2752..>3253
                     /locus_tag="PLB46_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77636.1"
                     /translation="MNKFICLLLTLLNTPIIFGQLDVQHKRDWPALTNSPNYYTVREN
                     YINYLYSLEMESGLLGVDLGADDLWTKFMRWDHIMKTRVDANGNYPKPGWLFEESARY
                     INAQKAFYEEGSRSAGWEPVGTAEVPGSGGGVGRVNVIMLDPVDPNILYAGSAGGGLW
                     RSPDLGG"
BASE COUNT         1003 a          622 c          529 g         1099 t
ORIGIN      
        1 ccatatcgca cgtaatactt tttaaaactt agcgactttg cgagaaacaa atacactttg
       61 aacgcaacat gtatttctcg ctaaggcgca aaggaaagtc gctataagat gtatttcgtt
      121 tatataaaaa accaatccaa gcgcgcaaag gtgttattgg tttaacagct ttgcgtgctt
      181 aaaaacattc catatcgcac gtaatacttt ttaaaactta gcgactttgc gagaaacaaa
      241 tacatctgcg tgaatccaaa acaaaaggag cagaaaaatg aactatcatt tcgctgctcc
      301 tttttttaat gctttatatc ttattccaca atattcaatt cctggatgat attagtcgct
      361 cccgcatatt tatctataat aaacaaggtg taacgaatat caagagaaat attacgacat
      421 ctgtttacat catacataat atcactcata gtgccttccc aaacacggtc gtaattggtg
      481 ccaattaatt caccattcgc attaattaca ggacttccac tattaccacc ggtggtatga
      541 ttgctggcaa taaaagcaat aggtaattcg ccttctttat tagcatatct gccaaaatta
      601 tttttttcaa ataaagcaat taatgatgca ggcatatcaa aatcttcatc accggcaata
      661 tatttttgct caattccttc gtcagttgtt tgatacgtgt agtaaacagc atcttttgcc
      721 gtataccctt ttacattgcc ataagtaacg cgtaatgtgg aattggcatc aggataaaaa
      781 tcacgatcag gctgcattaa tcgctgcgct ttcatgtatt cacgcatgta gataccaatt
      841 ttttcattca gcggatttat accggattta atttgtgtat catacatatt tgctacttca
      901 gcatataaac gataagcagg gtcattcaat aatttctttg cttttttgct attgaaatta
      961 ctcattgctt tttgcaactt ttcggcactg acaaaaaccg atttactgaa taacgcatca
     1021 gcccaggcat tataattatt ttgatatttt gcctgttctg ctttgaaata gtcactttgt
     1081 aatgccggag ctacattttc agcatataat ttcatcatgg ttgcaaatac ttctttatca
     1141 atttttacat aatatttgct ataaaaattt aaggaatttg tataaaaatt ggcagcttcc
     1201 tgctgataat attcaggtga tatggtatct gatgcagtga gtgcagcaat ctttttatag
     1261 ccggaagcaa aattgagcaa ttccactgca aaaaacgctt cactggaata atctactaat
     1321 tcagaatatg ctttatattc tgtataagca gcttccatat cttttagcaa attgcgataa
     1381 tcttttccct catgggtatt cgcccattgt tgaaatttag cttcatacgt ttgttttttt
     1441 gttacagcat catttagttt caacccaatc atttcacctt tccatttttt atacgcatta
     1501 gctaatcctt tagctttaga agcatacatt aacgtaacag tatcgttgcc actcatatat
     1561 ttattcataa tggctaaacg ttgatcacga cattcaatgc ggttgggatt ggtagtgtta
     1621 atggttaaat caactgcata agaagttaaa tattcctgag ttcttcccgg aaatccgtaa
     1681 accatggtaa aatcattttc ttcaacccct tttaacgaaa ttggaaaaga ataacgaggt
     1741 ttaaatggtt tattgtttgg gtcataagct gcaggtaaat tatctttccc tgcataaatt
     1801 ctgaaaatac taaaatcgcc agtatgtctt ggccacatcc aattatctgt ttcgccacca
     1861 aaattaccaa cactggaagg tggtgcacca actaatctga ggtctttaaa tgtttccgtt
     1921 aaaaataaat aaaattcgtt gccataataa aatgctctta ctgtagcatc ataatgcgta
     1981 ccttttttaa attgcgcttc caacttggca cttaattgtt ttattgtcat atttctggta
     2041 gcctcatcca tattttcatt caaataaggg ataatactat ccgtaacatc aaacatgcta
     2101 ataataaaag ttgcagttaa tccgggtgca ggaatttcct gttctttgtt tttcgcccaa
     2161 aatccatctc ttaaataatt attttctaat gtactcaaac tattaatgct ggaaaatcca
     2221 cagtggtgat tcgtcaacat taaaccttca ttagaaataa tttctcctgt acaaccacca
     2281 ttaaataaaa ccacagcatc tttcatgctg gttttattga tggaataaat atcttctgct
     2341 gtgagtttaa atccacgagc ttgcatgtcc tcaatattca attgctccaa taaaattggc
     2401 aaccacatgc cttcatcagc ttttacctga atgaaggtga aaattatcag aattatgcta
     2461 aacagttttt tcttcataag tgtcctacgt ttaaatacac ggcgaagata tttatcaaac
     2521 acttaaaatt taccttttat ttgttaaaaa ccagcgtaaa aacgctcatt ttgagtcaaa
     2581 aggcagtgtt tttaacaatc accaattaat gcagggattc ggtttttgtt taaagaagta
     2641 ataaacaagc agtatttagg cggatttggt ttcagaaatc tgtcattttt tatcaaatac
     2701 tattgttaca agtgatcact acttattttt aataaatttt tatttttaca tatgaataaa
     2761 ttcatttgct tactccttac gctcctcaac acaccaatta ttttcggaca attagacgtg
     2821 cagcacaaac gcgattggcc tgcactgact aattcaccaa attattatac cgtccgtgaa
     2881 aactatatta attacctcta ttccctggaa atggaaagtg gtttattggg tgtggactta
     2941 ggtgcagatg acctttggac gaaatttatg cgctgggacc atatcatgaa aacccgcgtt
     3001 gatgcaaatg gtaattaccc gaaacctggt tggttatttg aagaatctgc ccgatacatc
     3061 aatgctcaaa aagcatttta tgaagaagga agccgctcag ccggttggga acccgttgga
     3121 acagcagaag tccctggctc aggcggtggc gttggtcgag ttaatgtgat aatgctcgac
     3181 ccggttgatc ctaatatttt atatgcaggc tctgccggtg gtggtttatg gcgttcgccg
     3241 gatttaggtg gat
//
LOCUS       DAORKW010000436         3251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1653_length_3251_cov_4.871715, whole genome shotgun sequence.
ACCESSION   DAORKW010000436 DAORKW010000000
VERSION     DAORKW010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1653_length_3251_cov_4.871715"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PLB46_13750"
     CDS             <1..899
                     /locus_tag="PLB46_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRF77637.1"
                     /translation="DVDDTFTIDNTVFSTTFTHSNYAALKMKDALVDHFRNKFGRRPD
                     VNKLEPTYRFNIRIKENEVSIALDSSGESLHKRGYRTETHAAPMSEVQAAGLILLSGW
                     EVNQPLYDPMCGSGTIPIEAALIANQIAPGLIRKTYGFQKWKDYDASLFRQLMVAAAG
                     EVQQRRLAILGSDISSRNIVMAIKHAGNAMPDNSIKFFTKSFEDAEPPRDKGVLIINP
                     PYGERLQPEHIIEFYKTIGNVLKRKYAGFTAWVFSSNTEAAKFIGLKPSEKIKLKNGP
                     LDCLFLKFEIRDGKFVKNPMTD"
     gene            complement(1008..1994)
                     /locus_tag="PLB46_13755"
     CDS             complement(1008..1994)
                     /locus_tag="PLB46_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77638.1"
                     /translation="MNEEKKSENRGRGLYLILIFLLLGLNGWLFYNAFQNKEDRKKSA
                     AQIEEARILYSQLEVQYSQAKQQLQDQVGDFSQKDSLIAVYEAELDAKRNEIATLLKT
                     CNFFNGGVTTNQKKLDEVKEEVVSLEVDCSTYKAQLDELNAKYLALQEDFEELQILYE
                     KEVSRSEGLTFSKDSILEIGGFVLSKDITITGVRKKGNGNDSEGQNAKHTDRLKICFD
                     LLPNKLSAGKSQTFFVKIIGPTGKTLFNNAEGSGEFVNKEKPEDNLFSKAISVNYDGG
                     DVESHCMYWEQDEEFKAGTYTVKIYHNGYLSSRSSFTLKKPVIEDLMSRIGD"
     gene            complement(2361..>3251)
                     /locus_tag="PLB46_13760"
     CDS             complement(2361..>3251)
                     /locus_tag="PLB46_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRF77639.1"
                     /translation="SFTDDVIWVENTSTEVNIPNADKKEVAFVLFDPNSMIYAKVNFP
                     KTYTELKYQAFNAPNMMDRYDAIVLMRDLGLALKREDLITIYNKENFYGIKSEIISQL
                     ANDDNKSSLALLKTALTDKDPLVRRAVISNIQTLPKSLEKLTTALLADNNYTNIEIAL
                     TRLSTLNPDKINKYLEVTANEKGATNNIRITWLEIANRTNNNQYINELVNYSSSGYEF
                     RTRNAAMGALVNLNYCDQAVVVNLYNALLSSNHRLSTPARNALTKLKVTPEYNKLIKD
                     YYNSKTWSDWEQRKIGTIKD"
BASE COUNT          979 a          587 c          588 g         1097 t
ORIGIN      
        1 tggatgtaga tgatactttt acgattgata atacggtatt ttcaaccaca tttacgcata
       61 gcaattacgc cgcattaaaa atgaaagatg cgcttgttga ccatttcaga aataaatttg
      121 ggagaaggcc ggacgtaaat aaattggagc caacgtatag atttaatatc cgaataaaag
      181 aaaatgaagt aagtattgca ttagatagtt caggagaatc gttacataaa cgcggttatc
      241 gcaccgaaac acatgcggca ccaatgagcg aagtgcaggc tgctggatta attttattaa
      301 gtggttggga ggtaaatcag cctttgtatg acccgatgtg tgggagtgga acaattccta
      361 ttgaagcggc attaattgcc aatcaaattg ctccgggttt aattcgcaaa acatacggat
      421 ttcaaaaatg gaaagactat gatgcttcgt tgttcaggca attaatggtt gctgcagcag
      481 gagaggtgca acaacgaagg ttagctattt taggctcaga tattagttcc aggaatattg
      541 taatggcaat aaaacatgcc ggtaacgcta tgccggataa cagcattaag ttttttacca
      601 aatcgtttga agatgcggaa cctcctcgcg ataaaggggt attaataatt aacccgcctt
      661 atggtgagcg attacaacct gagcatataa ttgagtttta taaaacaatt ggtaatgtat
      721 taaaacgcaa atacgcagga tttacagcat gggtatttag cagcaataca gaagccgcaa
      781 aatttatcgg attaaaacct tctgaaaaaa ttaaattaaa aaatggcccg cttgattgcc
      841 tttttttaaa gtttgaaata cgtgatggta aatttgttaa aaatcctatg actgattaat
      901 acttgtcata tacacaataa aaaaaccgcg ccttttacga cgcggttcaa attataatct
      961 aactgctaag taatatgaat tggttctgtg tgtatgaatg gtatgtgtta atcgcctata
     1021 cgcgacatta aatcttctat taccggtttt ttcagtgtaa atgaacttct tgatgataag
     1081 tagccattgt gataaatttt tacggtatat gttccggctt taaattcttc atcctgttcc
     1141 caatacatac aatgactttc tacatcaccg ccatcataat taacactgat ggctttggag
     1201 aataaattat cttcgggttt ttctttgttt acaaattctc ctgaaccttc agcattatta
     1261 aataatgttt tacctgtcgg gccaattatt ttcacaaaaa atgtttgtga tttacctgcg
     1321 gacaatttgt tcggcagtaa atcaaaacaa atttttaagc ggtcggtatg tttagcattt
     1381 tgaccttcag aatcgttgcc attccctttt ttacgaacac cggtaatggt aatgtcttta
     1441 cttaatacaa atccaccgat ttctaaaatt gaatctttcg aaaaggtaag tccttcagaa
     1501 cgcgatactt ctttttcata taatatctgg agctcttcaa aatcttcctg caacgctaag
     1561 tattttgcat taagctcatc taattgagcc ttgtaagtag aacaatctac ctccagcgaa
     1621 actacttctt ccttcacctc gtctaatttc ttttggttgg tagttacacc accgttaaag
     1681 aagttacaag ttttaagtaa ggtggcaatc tcgttgcgtt tggcatccaa ttcggcttca
     1741 taaacagcaa tcaacgagtc cttttggctg aaatcaccaa cttgatcttg taattgttgt
     1801 ttggcctgac tgtattggac ctccaactgg ctgtaaagga tgcgggcttc ttcaatctgt
     1861 gcagcgcttt tcttcctgtc ctccttattt tgaaaggcat tatagaaaag ccagccattt
     1921 aggccgagca gcagaaagat gaggattagg tacagacctc tccctctgtt ttcagacttc
     1981 ttttcttcat tcatacgtga accgggtcag ttttttgttt tttgcctgtc atacttactc
     2041 aggctaaaac ttgacctata caaacttcgc agatataaat aatgcgctat aataatctaa
     2101 tgcacactac ttgctgatta ttagcgattt atgttgtaca actactgtat ccacatacat
     2161 gtacagtaca tagtcgaaaa atgaaaggct tgattttatt gggttttagc aaatttgagt
     2221 ggttgtataa atgcgatatc cacctcttgt gtacattttt attggcacgt gttgataacg
     2281 tgtggattta gtgttaataa tctccaaaac accagcccca aagggacagg tgaactatgt
     2341 cacaaaattt gaaacacttt ttagtcctta atggttccaa ttttacgctg ttcccaatcg
     2401 ctccaggttt tggagttgta ataatcctta atcagcttat tatactcagg agttaccttt
     2461 aatttggtta atgcgttacg ggctggcgtt gaaagtctgt gattgctcga aagtaaagca
     2521 ttatacaagt taaccacaac cgcctggtcg caataattca ggtttactaa tgctcccata
     2581 gctgcattgc gtgttctgaa ttcgtaacca ctgctgctgt aattaactaa ttcattgatg
     2641 tactgattat tgtttgtgcg attggcaatt tctaaccatg taatgcgaat attatttgtt
     2701 gcaccttttt cattggctgt tacttccaaa tatttattga ttttatcggg attcagcgtg
     2761 cttaaacgag ttaatgcaat ttcaatattg gtataattat tatcagccaa taaagccgtt
     2821 gttaattttt ctaatgattt tggtaaggtt tgaatattgg aaattacagc acgacgtact
     2881 aaagggtctt tatcggttaa cgctgttttt aataaagcta aacttgattt attatcatca
     2941 tttgccagct gactaataat ttctgattta ataccataaa aattttcttt gttgtaaatg
     3001 gtaattaaat cttctcgttt taatgctaaa cctaaatcac gcatcaaaac aatagcatcg
     3061 tatctgtcca tcatattggg cgcattaaag gcctgatatt ttaattcagt ataggttttc
     3121 gggaaattca cttttgcata aatcatcgaa ttaggatcga ataatacaaa cgcaacttct
     3181 tttttatctg cattgggaat gtttacttct gttgacgtat tttccaccca aattacatca
     3241 tcagtaaaac t
//
LOCUS       DAORKW010000437         3230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1672_length_3230_cov_3.952126, whole genome shotgun sequence.
ACCESSION   DAORKW010000437 DAORKW010000000
VERSION     DAORKW010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1672_length_3230_cov_3.952126"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /locus_tag="PLB46_13765"
     CDS             complement(<1..1862)
                     /locus_tag="PLB46_13765"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77640.1"
                     /translation="MHLKNTFLLSVLFGATTIFAQPSTELSIPQTYQAEQSGSLHFRG
                     KTKAMRETKENTAFEELFMDKRKHEDEARERSANTVVGEPDYFVEDPALQKDAEIATT
                     DISILSNWEGLKSKYLRSDNNIAVGPNHVVQMINSALFSSYIRVWNKTGDILINKANM
                     YDLVGEPDYGDPNIIYDEKADRWVITFLYSDSDARLIIMASVTNDPTGEWYYYDFETT
                     GGFPDYEKVAVWGNSYVVTTAQANPNVYLLNRESILNGTGASPVIKFLIPKFKNIGWQ
                     AASPVQQTGSIDLPEGEPAIIWRVWDNWWHPPYSAVDQLELFELDIDWATPLAATMTG
                     PIALPIEVYNSNLCGINSNTCLEQPGTTVRLWPLSNFITDKSKYMNFGDYQSIVGVHV
                     CRVNEAGTAGQRWYELRKYPGGEWYVYQQGTYSPTADHRFIGSISINEDGTIALGYNI
                     TSSTVYPGMRMTGRAVCDPINLMTAPEAISKNGTASNKTLDYGDYNSLVTDPVDGSFW
                     FSGQYNVSNKWSSNVVHFTVDGCLDEKSLPLQNTSLSDLTISPIPASNLITVGLQSDG
                     QSTVAIQIYDMSGKLVLQKSFMAGIGKNTFSFYINELASGAYVLKAVAVEKKNH"
     gene            complement(1999..>3230)
                     /gene="proS"
                     /locus_tag="PLB46_13770"
     CDS             complement(1999..>3230)
                     /gene="proS"
                     /locus_tag="PLB46_13770"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRF77641.1"
                     /translation="EGFAKECAVGTHDRLKTHPDKKGKLIVDPESRLEEELIIRPTSE
                     TIIWNCYRGWIQSYRDLPILINQWANVMRWEMRTRLFLRTAEFHWQEGHTAHATQEEA
                     VEETLKMLDVYADFMENYMAVPVIKGVKTANERFAGALDTYCVEAMMQDGKALQNGTS
                     HFLGQNFAKAFDVKFLNKEGKLEYAWATSWGVSTRMMGALIMAHSDDDGLVIPPRLAP
                     LHVVIVPIFKNAEQLAAITEKADALIKELKALNIIVKFDDSDTAKPGWKFAEYELKGV
                     PVRVAMGMRDVENNTVEIARRDTKEKTTVANENLGAYIQDLLEKIQQNMYDKALAFRT
                     ANTFVIDTWDAFTAQIEKGGFIYAHWDGTSETEDKIKELTKATIRCIPMDGIEEAGTC
                     VYSGKPSNKRVLFARAY"
BASE COUNT          956 a          709 c          525 g         1040 t
ORIGIN      
        1 ttatgatttt ttttttccac ggcaactgct tttaatacat atgccccact tgccagttca
       61 ttgatataaa aactaaatgt gtttttacca atcccggcca taaacgattt ttgtaaaact
      121 aatttacccg acatatcata aatctgaata gcaactgttg attgcccatc agattgtaag
      181 ccaaccgtaa taaggttgct ggcagggatt ggagaaattg ttaaatcact taacgaagtg
      241 ttctgcagtg gtaacgattt ttcatctaaa caaccatcca ccgtaaagtg cacgacatta
      301 ctggaccatt tgttgctcac gttatactga ccgctaaacc aaaatgaacc atcaaccgga
      361 tctgtcacca aactgttata atctccataa tccaatgttt tattggatgc tgtaccattt
      421 ttggaaattg cttccggtgc tgtcattaaa ttaatcgggt cacatacagc tctacctgtc
      481 atacgcattc ccggataaac tgttgagctg gttatattat aacctaaagc aattgtgcca
      541 tcttcattaa ttgaaatact accaataaaa cgatggtctg cagttggcga ataagtgcct
      601 tgctgataaa cataccattc accaccggga tatttacgta attcgtacca acgttgtccg
      661 gcagttcccg cttcatttac gcgacaaaca tgcacaccta caatactctg gtaatcacca
      721 aaattcatgt atttactttt gtccgtaata aaattactca agggccataa acgaactgtt
      781 gttcccggtt gttcgagaca agtattggaa ttaatgccac ataaattact gttgtaaact
      841 tcaattggta atgcaatagg accggtcata gttgctgcta atggtgttgc ccaatcaata
      901 tccaattcaa ataattccag ctgatcaact gccgaataag gtggatgcca ccaattatcc
      961 caaacacgcc aaataatcgc cggttcacct tccggtaaat ctattgaacc cgtttgttgc
     1021 acaggtgaag cagcctgcca tccaatattt ttaaatttag gaattagaaa tttaattacc
     1081 ggcgatgcac cagtgccatt taatatactt tcacgattta ataaatatac atttggattt
     1141 gcctgtgctg tggttactac ataactattt ccccacactg caactttttc ataatctgga
     1201 aaaccacctg ttgtttcaaa atcataataa taccattcac ctgttgggtc attagttacc
     1261 gatgccatta taattaatcg tgcatcacta tctgaatata aaaatgttat tacccaacgg
     1321 tctgcttttt catcgtaaat aatattgggg tcaccatagt caggttcgcc aaccaaatca
     1381 tacatatttg ctttattaat taaaatgtcg ccagttttat tccacacacg gatatacgaa
     1441 ctgaataatg cactgttaat catttgcaca acatgattcg gtccaacagc aatattatta
     1501 tccgaacgca aatattttga ttttaatcct tcccaatttg atagaataga aatatctgta
     1561 gtagcaattt cagcatcttt ttgtaatgca ggatcttcaa caaaataatc aggttcacca
     1621 acaacagtat tggcactgcg ttcacgagct tcgtcttcgt gttttctttt gtccataaac
     1681 aattcctcaa aagcagtatt ctcttttgtt tcccgcatgg cttttgtttt ccctctaaaa
     1741 tgtaaggagc cggattgttc agcctgatag gtttgtggaa ttgataattc ggttgaaggt
     1801 tgagcaaaaa tagttgttgc tccaaataat acactcaaca aaaaggtgtt tttcaaatgc
     1861 ataaatacat ggttaattat ttaaaagtag tgaaaataat cactacctaa aaatacggaa
     1921 attattttga ccgttaaaac aggcataaaa aaaacgacac cgaatgcgat gtcgtttatg
     1981 gttaatgttt taaattgatt aataagctct cgcaaataaa acgcgtttgt tacttggttt
     2041 gccactgtat acacaagttc ctgcttcttc aattccgtcc attggaatac aacgaatggt
     2101 agccttggtg agctctttta ttttatcttc agtttcggaa gtgccatccc aatgcgcgta
     2161 aataaatcca cccttttcta tttgagcagt aaatgcatcc caggtatcaa ttacaaaagt
     2221 gtttgccgtg cgaaaagcca gcgctttatc atacatgttt tgctgaattt tttccaacaa
     2281 atcctgaata taagccccta aattttcatt ggctacggtt gttttttctt tggtatcgcg
     2341 acgagcaatt tcaaccgtat tgttttctac atcacgcata cccatagcaa cacggacagg
     2401 aacacctttt aattcgtatt ccgcaaattt ccatcccggt ttagcggtat cgctgtcatc
     2461 aaattttaca ataatgttta aagcttttaa ttctttaatc agcgcatctg ctttttcagt
     2521 aattgcagct aattgttcag catttttaaa aattggaact attacaacat gcaatggtgc
     2581 taaacgtggt ggaattacca atccatcatc atcgctatgc gccataatta atgcacccat
     2641 cattcgggta gacacccccc agctggttgc ccaggcatat tccagtttgc cttctttatt
     2701 taaaaacttc acatcaaatg ccttcgcaaa attttgacct aaaaaatgag atgttccatt
     2761 ttgtaaagct ttaccatctt gcatcatagc ctcaacacaa tacgtgtcca aagcgcctgc
     2821 aaaacgttca tttgctgttt ttaccccttt aattaccgga accgccatgt agttttccat
     2881 aaaatccgca taaacgtcca gcattttaag cgtttcttca actgcttctt cttgtgtagc
     2941 atgagcggta tgaccttcct gccagtgaaa ttctgctgtt cttaaaaata gacgagtacg
     3001 catttcccaa cgcatcacat tcgcccactg gttaatcaaa atcggcaggt cgcgatagct
     3061 ctgaatccat ccgcggtagc agttccaaat aatggtctca ctggtagggc gaattatcaa
     3121 ttcctcttcc agtctacttt ccgggtcaac aatcagtttt ccttttttat caggatgcgt
     3181 ttttaagcgg tcatgcgtac ctacggcaca ctctttggca aatccttcac
//
LOCUS       DAORKW010000438         3229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1673_length_3229_cov_5.449590, whole genome shotgun sequence.
ACCESSION   DAORKW010000438 DAORKW010000000
VERSION     DAORKW010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1673_length_3229_cov_5.449590"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLB46_13775"
     CDS             <1..567
                     /locus_tag="PLB46_13775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRF77642.1"
                     /translation="TVLLHENMNWRQWIFFAVSFTGIIFINGFEKNEHTDLLMLGILG
                     AAFSGLAYNALRKTANHVPALVMVFYLPLCTIPIVTPFCVNNWVMPIGREWILIIAMG
                     LVTQVAQIYMTKAYQMEKAGTIANYAYLGVVFALGFGFIFFGERFNMIAIIGMIIVIV
                     GIILNFFYVNRVTSAKRFRAYFRYFPSW"
     gene            complement(568..1737)
                     /locus_tag="PLB46_13780"
     CDS             complement(568..1737)
                     /locus_tag="PLB46_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRF77643.1"
                     /translation="MNQDKYGLRGVSSQKEEVHKAIAHMDKGLYPNAFCKILPDFAAG
                     DAAYCNVMHADTAGTKTSLAYIYWKETGDISVWEGVAQDALVMNLDDMACVGITDNIT
                     ISSTIGRNKSVIPGEVLAAVINGTNRFIENMRELGVIIHHAGGETADVGDIVRTIDVG
                     ITAFGRIKRDELVVNNIQPGNVIVGLASFGQTSYETEYNGGMGSNGLTSARHDVFAKK
                     YAGLYPESYNQQIPDEVVYTGNKQLTDLIEVNGKLITAGKLVLSPTRTYLPVIKSIME
                     AHKSAISGMIHCSGGGQTKVLHFVDNVHIIKNNFFETPPLFQLIQEESKTDWKEMYKV
                     FNMGCRLEVYTDEKTAEAIITIANQFNIEAKIIGHVEAADSKKVTMHTAHGIFEY"
     gene            1944..>3229
                     /locus_tag="PLB46_13785"
     CDS             1944..>3229
                     /locus_tag="PLB46_13785"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRF77644.1"
                     /translation="MTFNERVLQEAEDKNVPLNDRIRFLGIFSNNQDELFRVRVAVLR
                     RMMRVQKSQLKVFDVNPQKTLTQIQKKVLVLKNRFDNAYLEILEDLKKHKIFILNEKE
                     INSQQGEIIRQYFNEKVHQRLFPIMLDNNKKMPLLNDKGIYLAVRMVKKDKPNATRYS
                     LIEVPDGDPSRFFMLPDDGDKKFIILLDDIIRYNLDAMYYIFNYDYIDAYTIKITRDA
                     ELDIHTDLSESLLEIIQKSVKRRTKGETTRFIYDAQMPEEMLQFFAKKMKLQRNDAII
                     PGARYHNFKDFMNFPDLGRKDLQYQPMPPVHHPLLNTNSSITSVLRQRDIFLHFPYQS
                     FHHIIDLLREAAINPNVTSIHMTLYRVAKNSSVVMSLINAARNGKQVTVVMELQARFD
                     EENNIFWSNKLQEEGVKVSFGVNNLKVHSKLCLITHV"
BASE COUNT          996 a          572 c          630 g         1031 t
ORIGIN      
        1 actgttttac tccacgaaaa tatgaattgg cggcagtgga tattttttgc cgtttcgttt
       61 acaggcatca tatttatcaa tgggtttgaa aaaaatgaac ataccgattt gttgatgttg
      121 ggtattttgg gtgcagcttt ttcgggatta gcatacaatg cgttgcgcaa aactgcaaat
      181 catgttccgg cactggtaat ggtattttat ttgcctctgt gcaccattcc aattgtgaca
      241 ccattttgtg taaataattg ggtaatgcca attggcaggg aatggatttt aataattgca
      301 atgggtttag ttactcaggt ggctcaaata tatatgacca aagcctatca gatggaaaaa
      361 gccggcacta tagcgaatta tgcctattta ggtgttgtat ttgccttagg ttttgggttt
      421 attttttttg gagaacgatt taatatgatt gccatcatcg gaatgattat cgtaattgta
      481 ggtatcattc tcaatttctt ctatgtgaac agagttacct ctgccaaaag atttagagca
      541 tattttcgtt attttccaag ctggtaatta atattcaaaa ataccatgtg ctgtatgcat
      601 ggttactttt ttactgtctg cagcttcaac gtgaccgatg atttttgctt caatattaaa
      661 ttgattggca atggtaataa ttgcttcagc tgttttttcg tcggtataaa cttctaaacg
      721 acagcccata ttaaatacct tatacatttc tttccagtca gtttttgatt cttcctggat
      781 taattgaaac aggggaggag tttcgaagaa attatttttg ataatatgca cattatcaac
      841 aaaatgtaac actttagttt ggcctccacc tgagcaatgt atcatacctg aaatggctga
      901 tttatgcgct tccattatgg actttatcac cggtaaatag gttctggttg gggaaagcac
      961 cagttttcca gccgtaatta gtttaccatt cacttcaatt aaatctgtca gctgtttatt
     1021 gccagtatac accacctcat caggaatttg ctgattataa ctttcaggat ataatccggc
     1081 gtattttttt gcaaaaacat cgtgtctggc gctggttaaa ccattgctgc ccattccgcc
     1141 attgtattcg gtttcataac ttgtttgccc aaaactggcc agtccgacaa ttacattgcc
     1201 cggttgaata ttgttcacca ccaattcatc gcgtttgata cgaccaaaag cggttattcc
     1261 aacatcgatg gtgcggacaa tatcacccac atcggcagtt tcgcctccgg catggtgaat
     1321 aattacgccc aattcgcgca tgttttctat gaaacggttg gtgccgttga tgactgcagc
     1381 aagtacttct cccggaatta cacttttatt gcgtccaatg gtagaggata tagtgatgtt
     1441 atcggtaatg cccacgcagg ccatatcatc gaggttcatc accaaagcat cctgagcaac
     1501 accttcccaa acgctgatat caccggtttc cttccaataa atgtaggcca aagaagtttt
     1561 agttcctgct gtgtcggcat gcatcacatt acaataagca gcatcgccgg cggcaaaatc
     1621 aggtaaaatt ttacagaaag cattgggata cagtccttta tccatgtgag cgatagcttt
     1681 atgcacttct tctttctgcg aggaaacccc acgtaatccg tatttatcct gattcatgat
     1741 gcaaagttac tgaaatgctt aaatctgctt atttgttagg tgaacaatta attaacaatt
     1801 aattaatcaa ctttacactc gcccttgctg agcatgctag tattttagcc aaagattaaa
     1861 tttgctctgt acaaggtgag tatatgagcg caaaaaagaa caaaaaattg aaactgaagt
     1921 atataaacag ggatatcagt tggttgacat ttaacgaaag ggtattgcag gaggctgagg
     1981 acaaaaatgt gcccttaaat gaccggattc gttttttagg tattttttca aataatcagg
     2041 atgaattatt tcgagtgcgc gttgctgtgc tgcgtagaat gatgcgagta caaaaatcgc
     2101 aattaaaggt atttgatgta aatcctcaaa aaacactgac gcaaattcag aaaaaagtat
     2161 tggtattgaa aaacaggttt gacaatgcat acctcgaaat tcttgaagac ttaaaaaaac
     2221 acaaaatatt tatcctcaac gaaaaagaaa taaatagtca gcagggagaa ataattcgtc
     2281 agtattttaa tgaaaaagtc catcagcgtt tgtttccaat catgctggac aataataaaa
     2341 aaatgccatt gttaaacgac aaggggattt acttagcggt gcgtatggtg aaaaaagata
     2401 aaccaaatgc cactaggtat tcgctgattg aagtgccgga tggtgatcca tcgcgttttt
     2461 ttatgttacc tgatgatggc gataaaaaat tcattatttt attggatgat attattcggt
     2521 ataacctgga tgcgatgtat tatattttta attatgatta catcgatgcg tatacaataa
     2581 aaattaccag agatgctgaa ttagatatac ataccgattt atcggagagt ttattggaaa
     2641 ttattcagaa aagtgtaaaa aggcgcacga aaggtgaaac aacacgtttt atttatgatg
     2701 cacaaatgcc tgaagaaatg ttgcagtttt ttgccaaaaa aatgaaattg caacgcaacg
     2761 atgccattat tccgggagcg cgttatcata attttaaaga ctttatgaat ttccctgatt
     2821 tgggtcgcaa agatttgcaa tatcagccaa tgccgccagt gcatcatcct ttattaaata
     2881 ctaactcaag tattaccagt gtgttgcgtc aacgggatat atttttacat tttccttatc
     2941 aaagttttca tcatataatc gatttactga gagaagcggc tattaatccg aatgtgacca
     3001 gtatacatat gactttgtat cgcgttgcta aaaattcgag cgtggtaatg agtttaataa
     3061 atgctgcccg caacggaaaa caagttacag ttgtaatgga attgcaggcg cgtttcgatg
     3121 aggaaaacaa tatattttgg tccaacaaat tgcaggaaga aggtgtaaaa gtttcatttg
     3181 gagtaaacaa cctgaaagta cattctaaac tatgtttaat tacacatgt
//
LOCUS       DAORKW010000439         3225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1674_length_3225_cov_4.858360, whole genome shotgun sequence.
ACCESSION   DAORKW010000439 DAORKW010000000
VERSION     DAORKW010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1674_length_3225_cov_4.858360"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..268
                     /locus_tag="PLB46_13790"
     tRNA            196..268
                     /locus_tag="PLB46_13790"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:229..231,aa:Met,seq:cat)
     gene            313..1626
                     /gene="der"
                     /locus_tag="PLB46_13795"
     CDS             313..1626
                     /gene="der"
                     /locus_tag="PLB46_13795"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRF77645.1"
                     /translation="MSFTVAIIGRPNVGKSTLFNRLTGERQAIVDDMSGVTRDRHYGI
                     VDWNGKQFDIIDTGGFVAHSSDVFEREIRRQVQIAIDEASLLIFMMDVTTGITDFDDD
                     VIRMLRKTDKTVFVVVNKVDNATRQLTATEFYAAGFENIFFVSSITGSGSGELLDAIT
                     AEIHEEDASLLASQQSLPRFAIVGQPNVGKSTLLNALTGQERTLVTDIAGTTRDAIHT
                     HYKLFEKEFILIDTAGIRKKASVKEDIEFYTVIRAIKAVDESDVCLLLIDANTGIESQ
                     DMAILQMIEKKKKGLVILVNKWDMVEKETNTLLRYEETIREKIAPFRDVPIIFISAIE
                     KTRIFKAMEMALQVYENKHLKISTSKLNEVMQLALENYHPPAVRGNYIKIKYVTQMPA
                     QVPTFVFFTNFPNDIREPYRNYLENQLREHFSLTGTPVNIFFRKK"
     gene            1681..1926
                     /locus_tag="PLB46_13800"
     CDS             1681..1926
                     /locus_tag="PLB46_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77646.1"
                     /translation="MKKVSYLAIALVIMGFVACKSNKAGEGETTTTDTLTTQVENGAS
                     EAIDNAAKVDEVLNQDSDDELFGTDVDDAAVEGATTK"
     gene            complement(1993..>3225)
                     /locus_tag="PLB46_13805"
     CDS             complement(1993..>3225)
                     /locus_tag="PLB46_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF77647.1"
                     /translation="VDDTILVVAALSGGPSAKLGIQAGDKIIKIDDSLVAGQKIISEK
                     VIKKLRGKKGTEVKVDIKRQGEKNLIPFTIVRDVIPITSIDAAYMVDDKIGYIKVNKF
                     SDKTPIEFDDALMNLKNSGMKQLILDLRGNPGGYLTGAVALADEFLAGTPLIVYTEGR
                     AVGRSDYNAGKNGLFESGNLVVLINEYSASASEILAGALQDNKRAKIVGRRSFGKGLV
                     QQVYDLNDSSAIRLTIARYYTPSGKSIQRAYDDGVDAYYDQYMSIIMNGGELPDSLKG
                     NKNIDWGIHPDVFVPIDTSPINQTLNYLLNRSYVQQFSYSTYSANPKSFAAYTDMRNF
                     ASTYEVTDAMFNKFKQFVIAHDPDKGVSESEIIAARPKIEIAIKAYLSRQQWGDNGYY
                     LNMNKLDDTFNKAIEQVK"
BASE COUNT         1006 a          617 c          589 g         1013 t
ORIGIN      
        1 aacttataag ttattaacat acaacacttt gagccagtac agtatataat ttgttttttt
       61 ggggatataa agcaatattt atacttgaat tctgtatcat ttttcactta taaaaagtct
      121 cattacaaaa tatcttattc tttactcaaa attaatgttg gttttcacac aaaagaatgt
      181 aattttgcac cacatcgcgg gatggagcag ttggtagctc gtcgggctca taacccgaag
      241 gccacaggtt cgagtcctgt ccccgctaca acgaaaggct ggaaatttcc ggccttttta
      301 atttaattga atatgagttt tactgtagcc attattggaa gacccaacgt tgggaaatcg
      361 acactgttca acagactgac tggtgaacgc caggctattg tcgacgacat gagtggggtt
      421 acacgcgatc gccattatgg tattgtagat tggaacggaa aacaattcga tattatcgat
      481 accggtgggt ttgtggcaca tagctccgat gtttttgagc gcgaaatccg ccgccaggtg
      541 caaatcgcca ttgatgaagc ttcattgctc atttttatga tggatgtaac tacgggtatt
      601 actgattttg acgacgatgt aattagaatg ttgcgcaaaa ccgataaaac ggtatttgtg
      661 gtggtaaata aagtagataa tgctaccagg cagttaactg ctacagagtt ttatgcagca
      721 gggttcgaaa atatcttttt tgtttcttcc attacaggaa gtggttccgg cgaattgtta
      781 gatgccatta ctgctgaaat tcacgaagag gacgccagtt tattggcttc tcagcaaagt
      841 ttaccacgtt tcgccattgt tgggcagcca aacgttggaa aatctacctt actaaacgca
      901 cttacaggtc aggaacgcac cttggttacc gatattgccg gtacaacacg tgatgccatc
      961 catacccatt acaaattatt tgaaaaggaa ttcatcttaa ttgatactgc cggtatccgg
     1021 aaaaaagcat cagttaaaga agatattgaa ttttataccg ttatcagggc aattaaagct
     1081 gttgatgaaa gcgacgtttg tttattgcta attgatgcca atacagggat tgaaagtcag
     1141 gacatggcca ttcttcaaat gattgaaaag aagaaaaagg ggctggtaat tctggtaaat
     1201 aaatgggata tggtggagaa agaaaccaat accctcctta gatacgaaga aaccatccgc
     1261 gaaaaaattg cgcctttccg tgatgtgccc atcattttca tctccgcaat agaaaaaaca
     1321 cgcattttta aggctatgga aatggcttta caggtgtatg agaataaaca tctaaaaatt
     1381 tctacctcta aattgaatga ggtaatgcaa ttggctttag aaaattacca tccaccggca
     1441 gtgagaggta actatatcaa aatcaagtat gtaactcaaa tgccggctca ggtgcctact
     1501 tttgtatttt tcaccaattt tccaaacgat atacgcgaac cttaccgaaa ttacctggaa
     1561 aatcagctaa gagaacattt ttcgctgaca ggaactccgg taaacatttt cttcagaaag
     1621 aaataagcgt tattattttt tataattttg catcatacca ctaaacaaaa tcagaacaaa
     1681 atgaaaaaag tatcttattt agctatcgcc cttgtaatca tgggttttgt tgcttgtaaa
     1741 tcaaacaaag ccggtgaagg agaaacaacc acaaccgata cacttactac tcaggtagaa
     1801 aacggtgctt ctgaagctat tgacaatgct gccaaagttg atgaagtgct taatcaggat
     1861 tcagatgacg aattattcgg aactgatgtt gatgatgctg ctgtagaagg tgctactaca
     1921 aaataataaa agacctaaaa ctaaaaaacc ccgaatttcg gggttttttt atgcttgtaa
     1981 ttttatcaat aattatttga cttgttcaat ggctttgtta aacgtatcat ctaacttatt
     2041 catatttaaa taataaccat tatcacccca ttgttgacgc gataaatagg ctttaatggc
     2101 aatttcaatt tttggtcgcg cagcaattat ttcactttca ctcactcctt tatcagggtc
     2161 gtgcgcaatt acaaattgtt tgaatttatt aaacatggca tctgtaactt cataggttga
     2221 tgcaaagttg cgcatgtcag tataggcagc aaatgatttt ggatttgctg aatacgtaga
     2281 ataggaaaac tgctgaacat atgagcggtt aagcaaataa tttaatgttt gattaattgg
     2341 agatgtatca attggcacaa acacatcagg gtgaattccc caatcaatat ttttattacc
     2401 ttttaaacta tcaggtaatt ctccaccatt cataatgatg ctcatatatt gatcataata
     2461 agcatcaaca ccatcatcgt aagcacgttg aatactcttg cctgaaggtg tgtaataacg
     2521 agcaatagtt aagcgaatgg ctgaactgtc gttgaggtcg taaacctgct gtacaagccc
     2581 ttttccgaaa cttcttctac caacaatttt ggcgcgttta ttatcttgta atgcacctgc
     2641 taatatttca cttgcggaag cagaatattc attaattaat accactaaat tgccactttc
     2701 aaataatccg ttttttcctg cattgtaatc acttctgcca actgctctgc cttcggtata
     2761 tacaatcaac ggtgtgccag ctaaaaattc atcagcgagg gcaactgctc cggttaaata
     2821 tccgcccggg tttcctctta aatccaatat taattgtttc atgcccgaat ttttcaaatt
     2881 cattaaggca tcatcaaatt cgattggtgt tttatctgaa aatttattta ctttaatata
     2941 gccgatttta tcgtcgacca tatatgccgc atcaatactg gtaatcggaa ttacatcgcg
     3001 tactatggta aacggaataa gatttttttc accctgacgt tttatatcta ctttaacttc
     3061 tgtgcctttt tttccgcgca attttttaat cactttttca ctgataattt tttgcccggc
     3121 aaccaaactg tcatcaattt taataatttt atcacctgcc tgaataccca atttagctga
     3181 tgggccacca cttaatgcag caaccactaa aatggtatca tctac
//
LOCUS       DAORKW010000440         3217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1687_length_3217_cov_4.061037, whole genome shotgun sequence.
ACCESSION   DAORKW010000440 DAORKW010000000
VERSION     DAORKW010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1687_length_3217_cov_4.061037"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PLB46_13810"
     CDS             complement(<1..886)
                     /locus_tag="PLB46_13810"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HRF77648.1"
                     /translation="MKNTYLDLIQQTFYFPQDGFEVKNNYLYFNGIPLKQLIEEYETP
                     MRITYLPKISENIQRAKKYFNDAIRKLNYPGNYNYCYCTKSSHFAHVLIEALKNDIYL
                     ETSSAFDIDIIERLYLNKMLNKDTLIVCNGFKTKIYTQRISKLANLDFNIMPVLDNME
                     ELEAYENTIIYDCDLGIRIASEEQPEFQLYTSRLGIRYKEIKDFYLNKIKPNPKFKLK
                     MLHFFINSGIKDNIYYWNELSKLLKVYVELKRVCPELDSLNIGGGWPFRSSLGFEYDY
                     AYMAEEILNKINKTSTTEQ"
     gene            958..1911
                     /locus_tag="PLB46_13815"
     CDS             958..1911
                     /locus_tag="PLB46_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HRF77649.1"
                     /translation="MKRVKVISVNSELGAGTRGAGLGFDAIRVAAWTKGSRYFKEHPP
                     VILQSNNDEVLDDIETAYSVKIQFIVEMYNKIAEVVVDCMKKKEFPVIISGDHSNAGG
                     TIAGLKMAYPKDTLGVIWIDAHADLHSPYTTPSGNLHGMPLAASTALDNKVCQINQPE
                     KKTIEAWEKLKKIGGISPKITFKDLIYIATRDMEKPEKFILKKNNVRIYSVQKMRETG
                     IQFVIEDAMKRLAHCDRIYVSFDVDSMDANVSRGTGTPVKSGLYESEAFQLVSGLAKE
                     PKVCCMEITEVNPTLDNKKNMMAEIALEILEQFTGEIEKRL"
     gene            1908..>3217
                     /gene="argS"
                     /locus_tag="PLB46_13820"
     CDS             1908..>3217
                     /gene="argS"
                     /locus_tag="PLB46_13820"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRF77650.1"
                     /translation="MMELIAQLKKDAVAVVNKLYNQQVDATIIKIDKTPPEFTGELTL
                     VIFPFVKISKKNPEVTANEIGEALMAINTTIAGYNVVKGFLNISFTDAYWSGVLQHIH
                     NTKNYGVLAATGKKVVLEYCGPNTNKPLHLGHVRNVLLGFAAANILAAAGHAVHKVNI
                     LNDRGIAICKSMVAYERNGNGSTPTSTGIKGDHFVGHYYIEFAKIHEAEMAALIAAGK
                     TKEEAEKNTPIYLAAQEMLRKWEAGDAAVLDLWNTMNNWVYEGFGVTYQKIGIDFEKE
                     YKESEFYLHGKKMVQEGLTQGAFFKKEDGSIWVDLTSDGLDQKVLLRADGTSMYITQD
                     MGVAEARYGDFKMDTSVYVVANEQDYHFKVLKLVLQKLGRPYGDGIYHLSYGMVDLPE
                     GKMKSREGTVVDADDLISEMTEEAKTFLAASEKSAAFNEEEKQQ"
BASE COUNT         1044 a          576 c          682 g          915 t
ORIGIN      
        1 gttgttctgt tgtggatgtt ttgtttattt tattcagaat ttcttctgcc atataggcat
       61 aatcgtattc aaaacccaat gaacttctaa acggccatcc accgccaata tttagcgagt
      121 cgagttccgg acaaacacgt tttaattcaa catacacctt gagcaatttc gataactcgt
      181 tccaataata aatattatcc ttgatgccgc tattgataaa aaagtgcaac atctttaatt
      241 taaatttcgg attaggtttt attttgttga gataaaaatc tttaatctct ttatatctga
      301 tgcccagtcg cgaagtatac aattgaaatt ccggttgttc ttctgatgca attcggatac
      361 ctaaatcaca atcgtaaata atggtatttt catacgcttc caattcctcc atattatcca
      421 acaccggcat aatattaaaa tcgaggttgg ctaatttgct gatgcgttgg gtgtaaattt
      481 ttgtcttaaa tccgttacac acaattaaag tatccttatt cagcattttg ttgagataca
      541 aacgctcaat aatatcgata tcaaatgccg aagaagtctc cagataaata tcatttttga
      601 gcgcttctat cagcacatgg gcaaaatgcg aacttttggt gcaataacaa taattgtagt
      661 tgccgggata gttaagcttg cggatagcat cgttaaaata ctttttggcc cgttgaatat
      721 tttccgaaat tttcggcaaa taagtaatcc gcatcggggt ttcatactct tctatcagct
      781 gtttcagcgg tattccgttg aagtagaggt agttgttttt cacttcgaat ccatcctgcg
      841 ggaagtagaa cgtctgctgg atgagatcaa ggtaggtatt tttcaatttt tattggttcg
      901 tttgattgtt acaattataa atttttctag cagtgcaaaa ataataactt tgcatatatg
      961 aaacgtgtaa aagtaatatc cgttaacagc gaattgggtg ccggcacccg tggcgcggga
     1021 cttggctttg acgccatccg tgttgccgcc tggaccaaag ggtcaagata ctttaaagaa
     1081 caccctccgg tgattctgca aagcaacaac gacgaggtgt tagacgatat tgaaactgcc
     1141 tactcggtga aaattcagtt tattgttgag atgtacaaca aaatagccga agtagtagtg
     1201 gattgcatga aaaagaaaga gtttccggta atcatttccg gcgaccatag caatgccgga
     1261 ggcactattg ccggacttaa aatggcctat cctaaagata cccttggggt aatttggatt
     1321 gatgcccatg ccgacctgca ttcgccttat acgacgccat cgggtaacct gcatggtatg
     1381 ccattggcgg cttctacggc tttggacaat aaagtttgtc aaattaacca gccggagaaa
     1441 aaaaccattg aagcctggga aaaattgaaa aaaattggtg gtatttcacc gaaaatcacg
     1501 tttaaggacc tgatttatat tgccacccgc gacatggaaa aaccggaaaa atttattctc
     1561 aaaaaaaata atgtccgcat ttattccgta caaaaaatgc gtgaaaccgg aattcagttt
     1621 gtaattgaag atgccatgaa acgtttagca cattgcgacc gtatttatgt atcgttcgat
     1681 gtagacagca tggatgccaa cgtttcgcgc ggaacaggaa caccggttaa aagcggatta
     1741 tacgaaagtg aagcatttca gttagtgagc ggactggcaa aagaaccaaa agtgtgttgt
     1801 atggaaatta ccgaagtaaa tccgacactc gataacaaaa aaaatatgat ggccgaaatt
     1861 gccctcgaaa tattggagca atttaccgga gaaattgaaa aaagactatg atggaattaa
     1921 ttgcgcaact taaaaaagat gcggttgcgg tagtaaataa attatataat caacaggttg
     1981 atgccactat tattaaaata gataaaacgc ctcctgaatt tacaggagaa ctcaccctgg
     2041 ttatttttcc atttgtaaaa atcagtaaaa aaaatcctga agtaactgcc aacgaaattg
     2101 gcgaagcatt aatggcaata aatacaacta ttgccggcta taatgtggta aaaggatttt
     2161 taaatatcag ctttaccgat gcgtattgga gtggtgtttt acaacatatt cacaacacca
     2221 aaaactatgg tgtattagca gctaccggca aaaaagtagt gttggaatat tgcggtccca
     2281 ataccaataa acctttgcat ttaggtcatg tgcgcaatgt attactcggt tttgcagcag
     2341 ccaatatttt agccgcagca ggtcacgcag tacataaagt aaatatcctc aacgacagag
     2401 gtattgccat ttgtaaaagt atggtagctt atgagcgcaa tgggaatggt tcaacgccga
     2461 cttcgacagg tataaaaggc gaccattttg tcggccatta ttatattgaa tttgccaaaa
     2521 ttcacgaggc tgaaatggcg gctttaattg cagcaggaaa aacaaaagaa gaagcagaaa
     2581 aaaacacccc aatatattta gctgcccaag aaatgttgcg caaatgggaa gccggtgatg
     2641 cagcagtttt ggatttatgg aataccatga acaactgggt gtatgagggt ttcggcgtta
     2701 cctatcaaaa aattggtatc gatttcgaaa aagaatacaa ggagagtgaa ttttatttgc
     2761 acggtaaaaa aatggtgcaa gaaggactta cccaaggggc atttttcaaa aaagaggatg
     2821 gcagtatttg ggtagattta acttccgacg ggctggatca aaaagtatta ctcagagcag
     2881 atggtaccag catgtatatt acccaggata tgggtgttgc tgaagcgcgt tatggcgact
     2941 ttaaaatgga cacttcggtg tatgtggttg ccaacgaaca ggattatcat tttaaagtac
     3001 tgaaactcgt tttacaaaaa ttgggcaggc cttatgggga tggtatttac cacctcagtt
     3061 atgggatggt agatttaccc gaaggcaaaa tgaaaagtcg cgaaggtacc gtagtggatg
     3121 ctgacgattt aatcagtgaa atgactgaag aagccaaaac ttttttagcc gcatcagaaa
     3181 aatcggcagc atttaatgaa gaggaaaaac agcaatt
//
LOCUS       DAORKW010000441         3215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1691_length_3215_cov_5.015190, whole genome shotgun sequence.
ACCESSION   DAORKW010000441 DAORKW010000000
VERSION     DAORKW010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1691_length_3215_cov_5.015190"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /gene="kynU"
                     /locus_tag="PLB46_13825"
     CDS             <1..1090
                     /gene="kynU"
                     /locus_tag="PLB46_13825"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HRF77651.1"
                     /translation="LTDWQNLGVEGHLHAKNPWLYYHHFLTDATARLVGAKPLEVVVM
                     NSLTVNLNLLMISFYRPTPKRYKIMMEYMAFPSDQYAVENQVKFHGYNPNDAIIELMP
                     REGENCIRTADILAKIDEHKDELALIMIGGVNYYTGQLFDMVTITSHAKKCSTEIVVG
                     YDLAHATGNVQLHLHDWDVDFATWCSYKYLNSGPGGTSGVFVHEKHAENNLLPRLSGW
                     WGNDESTRFKMQKGFIPQQGAAGWQMSNAQVLSMAAHRASLAIFDEVGMDKLIEKSKL
                     LTGYLEFLLLNGKRKDFKIITPEDVAQRGCQLSIVMNENGKKTFDALTQNGVIADWRE
                     PDVIRVAPVPLYNTFEDVLRFAEIFHAV"
     gene            complement(1160..1744)
                     /locus_tag="PLB46_13830"
     CDS             complement(1160..1744)
                     /locus_tag="PLB46_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HRF77652.1"
                     /translation="MRKTTTYLLFFALSGIALYSCQVKTEHASEENPASTTTTEDPIK
                     RGAFLVQVGGCNDCHTPKMMTDKGPAFDMSKMLSGHQANIPTPAYDKSLLTDWVLMHP
                     QLTAAVGPWGVSYAANLTPHETGLGNWSEENFLKAMKEGKHLGMDNQRPIMPPMPWQG
                     LSMWPDEDLKAVFVYLKSIPPIDNLVPAYQPAAM"
     gene            1854..3197
                     /locus_tag="PLB46_13835"
     CDS             1854..3197
                     /locus_tag="PLB46_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF77653.1"
                     /translation="MKSVKNIAIMGAGLVGSLLSLYLKKRGYEVTVYERRPDLRKTGA
                     AGGRSINLAISERGWKGLAGVGLEAEIRKMAIQMPGRMIHDMQGNLNFQPYGKPGEAI
                     NSVSRGDLNIALIDAAEANGIQFIFNQRVLETDLAGTYAMMEDGNKGGTYKLQPDLII
                     GADGAFSLVRYTMMKTDRFDYSQTYEAHGYKELTIPAGPDNSFLIEKNALHIWPRKNF
                     MLIALPNMDGSFTCTLFYNLDGEQSFKTLTTEKDVEVFFRTYFADAMAIMPTLYHDFF
                     ANPTGLLMTVKCFPWSHGNTMLIGDAAHAIIPFYGQGMNCGFEDCSVLNELLGTYNDD
                     WEKTIPAYQNARKPNSDGIADLAKLNFIEMRDLVADPDFQFKKQIAAKVSATYPDKFI
                     PVYSMVSFSNMPYSEALKEYRRQDEVLSAVLLLPEIREKWDTTYFQEIAGRLFNK"
BASE COUNT         1020 a          564 c          673 g          958 t
ORIGIN      
        1 actcacagac tggcaaaatt taggagtgga aggacattta cacgcgaaaa atccatggtt
       61 gtattatcac cattttttaa cagatgctac cgctcggtta gtcggcgcaa aaccattaga
      121 ggttgtggta atgaattcgt taacggtaaa tcttaatctt ttaatgataa gtttttatcg
      181 cccaacacct aaacggtata aaatcatgat ggaatatatg gcatttccat cagaccaata
      241 tgcggtagaa aatcaggtga aatttcatgg ttataatccc aacgatgcta ttatagagtt
      301 aatgccacgt gaaggagaaa attgtatacg cacagctgat attttagcga aaatagatga
      361 gcataaagat gaattggcgc ttatcatgat tggtggtgta aattattata ccggtcagtt
      421 atttgatatg gtcacaatta catctcacgc aaaaaaatgt tcaacagaaa ttgttgtcgg
      481 atatgattta gcgcatgcaa caggtaatgt tcaattgcat ttacacgatt gggatgttga
      541 ttttgcaacc tggtgttctt ataaatattt gaattcaggt cccggcggaa ccagcggcgt
      601 tttcgtacat gaaaaacatg cggaaaataa tttgcttccg cgattaagtg gttggtgggg
      661 aaatgatgaa tcaacaagat ttaaaatgca aaaaggattt attccgcaac agggcgctgc
      721 cggctggcaa atgagtaacg ctcaggtttt gtcaatggca gcacacagag catcattagc
      781 tattttcgat gaagtaggaa tggataaatt aatagaaaaa agtaaattac taaccggcta
      841 tttggaattt ttattactca acggaaaaag aaaagatttt aaaattatca cacctgaaga
      901 tgttgcacaa cgtggttgtc aattgtccat tgtgatgaat gaaaacggta aaaaaacatt
      961 tgatgcactt acacaaaatg gtgttattgc cgattggcgt gaaccggatg taattcgtgt
     1021 tgcacctgta cctttatata atacatttga agatgtactc cgttttgcgg agatttttca
     1081 cgcagtataa aaaaaatgag caatacatct gttgataatg atgcattgct caccaaataa
     1141 tttattcgct tgtattaaat tacatagctg ccggttgata agcaggtacc aaattatcga
     1201 ttggtggaat agatttcaaa taaacaaaaa cggcctttaa atcttcatca ggccacatgc
     1261 tcaaaccttg ccaaggcata ggtggcataa ttggtcgctg attgtccata cctaaatgtt
     1321 taccttcttt catcgccttt aaaaaatttt cttcagacca atttcctaaa ccagtttcat
     1381 gtggagttag attggcagca taagaaacac cccaaggtcc tacagcagca gtcagttgcg
     1441 gatgcataag tacccaatcg gtaagaaggg acttgtcgta agccggtgtt ggaatatttg
     1501 cctgatgacc cgacaacatc ttgctcatat caaatgcagg acctttgtct gtcatcattt
     1561 ttggtgtatg gcaatcgtta catccgccaa cctgaactaa aaaagcacca cgcttaattg
     1621 gatcttcagt agttgttgtt gaggcaggat tttcttcaga agcatgttct gtttttacct
     1681 ggcagctgta aagtgctatg cctgaaagag caaaaaacag caggtaagtg gttgtttttc
     1741 tcataagtaa ttgattttca cggaaattta cgaacaattt tgctctttga gttttatcat
     1801 cttttacaag tggcaaatag gtttattttt gccaaagcgt ttaatcaata cttatgaagt
     1861 cagtaaaaaa cattgcaatt atgggagcag gccttgtggg ctcgctttta agtttatacc
     1921 tcaaaaaacg tggatacgag gttactgttt atgaaagaag gcccgatttg aggaaaacag
     1981 gagcagcagg cggtaggtct atcaatctgg ccatcagcga acgtggctgg aaagggttag
     2041 ccggcgttgg tttagaagcc gaaatcagga aaatggctat ccaaatgccc ggtcgtatga
     2101 ttcacgatat gcaaggaaat ctaaattttc agccatatgg taaacctgga gaagctatca
     2161 actcagtttc cagaggtgat ttaaatattg ctttaattga tgctgctgaa gcaaatggta
     2221 ttcagtttat ttttaaccag cgtgtattgg agactgacct tgccggaact tacgccatga
     2281 tggaagatgg caataaaggt ggtacgtata aattacaacc cgatttaata attggtgccg
     2341 atggtgcatt tagtttggtg cgatatacca tgatgaaaac cgaccgtttt gattattcgc
     2401 aaacttatga ggcgcatgga tataaagaat taactattcc tgccggtccg gataattctt
     2461 tcctcatcga aaaaaatgca ttacatatct ggccacgcaa aaatttcatg ttgattgcat
     2521 tacctaatat ggatggcagt tttacctgca cattattcta taatctcgat ggcgaacaat
     2581 cgtttaaaac attaactacg gaaaaagatg ttgaagtgtt tttccgcacc tattttgccg
     2641 atgctatggc gataatgcca acattatatc acgacttttt tgctaaccca acaggtttac
     2701 tcatgaccgt taagtgtttc ccatggagcc atggaaacac aatgctgata ggagatgcag
     2761 cccatgccat tattcctttt tacgggcagg gaatgaattg cggatttgaa gattgttctg
     2821 ttttaaatga attattgggt acctacaacg acgactggga aaagacaatt ccggcttacc
     2881 aaaatgcacg caaaccgaat agtgatggaa tagccgattt ggcaaaactt aattttatag
     2941 agatgcgcga tttggtagcc gaccctgatt ttcagttcaa aaaacaaatc gcagcaaaag
     3001 taagtgcaac ttatcctgat aaatttattc cggtatatag tatggtttcc tttagtaata
     3061 tgccttatag tgaagcatta aaagaatacc gcagacagga tgaagtgctt tcagctgtgt
     3121 tattgttgcc cgaaatacgg gaaaaatggg acacaacata tttccaggag attgctggtc
     3181 ggttatttaa taaataaaaa aactaaatac taata
//
LOCUS       DAORKW010000442         3215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1692_length_3215_cov_4.894937, whole genome shotgun sequence.
ACCESSION   DAORKW010000442 DAORKW010000000
VERSION     DAORKW010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1692_length_3215_cov_4.894937"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLB46_13840"
     CDS             <1..339
                     /locus_tag="PLB46_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77654.1"
                     /translation="IPLTKLALDQGGYDWGVLAYAVGFGGSMIWFGSSAGVAITNMYP
                     EAKSVGAWLKGGWFVIVGYIVGFIFLMLTFGWHPHAPHKATNQADTEHQAAVITPTSE
                     TSMVNSTSIR"
     gene            387..959
                     /locus_tag="PLB46_13845"
     CDS             387..959
                     /locus_tag="PLB46_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF77655.1"
                     /translation="MSNLSDEELLSAYKRSGESKYAGELFKRYAHLVLGVCMKYLKNM
                     DEAQDTTMFVFEKLLSELRQTDVQHFKSWLYMVAKNQCLMHIRKQKRHDIVTFDTENG
                     ENEDGNSMEIGAIEHLDDVDLKEVQLQQLEEGIKTLNHEQQECISLFYLQDKSYVEVA
                     EITGYDLNKVKSYIQNGKRNLKIYLEKRNV"
     gene            952..2091
                     /locus_tag="PLB46_13850"
     CDS             952..2091
                     /locus_tag="PLB46_13850"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRF77656.1"
                     /translation="MSEFKLHIEKGGYVSKTTLLAYLNNELNAADKLYVEHLISEDPF
                     LQDAIEGLRGADSKLVEATLNAIYKDVDVITGTKKPFTITATIKKYAAAAMLIVFFGL
                     TFLIMNVLNKSQTNDAIAIENEVVEAQQSEANGNADGMGAGDLNSDTSNESEVAKTVR
                     SKFNNANEIPTTSNPEIVYDNSSSVSEGVSETKYLKEDMVLADEEINTDKDKVVLYDN
                     KVEVKSTTGNAQVLTSGPVVVGEVSYTESVTLSTVSEKKVLDKAKPASTDDADFGYIA
                     ADSIATEITKPEYPGGLDALNKYLAKSIDYKNTGEVNGTIYVQFIIDENGKINSPVIT
                     IGINEKVNAEVIRSINRMPKWKPAHVNNKNINVQISMPIYFGSQN"
     gene            2111..3043
                     /locus_tag="PLB46_13855"
     CDS             2111..3043
                     /locus_tag="PLB46_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77657.1"
                     /translation="MNPKIKLLFVLSIFIISLCLSADYIFSAFLTKTTPVAESNKIYR
                     LTNEQHNNEIPIFGSSRAEKGYYCDNINPNCYNYGFPNQSFNVTALLLNFELKKNKTT
                     PIIIDIHHDFFQHDAYTNINLATYLPFVNSNDSITAFLERNKRLKPYHYVPGTRYFGY
                     YTTYLEPIVENKMKIGNALYLKGGVFDTKKGNSRSIQKRIDNRLDKKLAFTVTPDNDK
                     QLKQLITSRPDRKFIFVVSPYHWSAKSTVANFDEMLNYFNGLAKNYPNVHFIYFNTDK
                     YGDELFKDTVHLNEAGAKQFSAELQTHLQALDIY"
     gene            complement(3040..>3215)
                     /locus_tag="PLB46_13860"
     CDS             complement(3040..>3215)
                     /locus_tag="PLB46_13860"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77658.1"
                     /translation="LKKDVTANEEDIIQHCKKNLAPFKVPSVVRFVSSIPKSPSGKVL
                     KRLLREEDISSRN"
BASE COUNT         1115 a          561 c          607 g          932 t
ORIGIN      
        1 attccactca ccaaactggc cttagatcag ggtggttacg actggggtgt actggcctac
       61 gcagttgggt ttggaggcag tatgatttgg tttggttcca gtgcaggtgt tgccattacc
      121 aatatgtatc cggaagcgaa atctgtcgga gcctggttaa aaggtggctg gtttgttata
      181 gttggttata tcgttgggtt cattttcctg atgcttacat ttgggtggca tccacatgcc
      241 ccacataagg ctacaaatca ggcggatact gaacatcagg cagctgtaat caccccaact
      301 tctgaaacaa gcatggtaaa ttcgacatca attcgttaat tttatctgat tctgtgttac
      361 aactactttt ctcccgttca aaaaaaatga gcaacctcag cgatgaggaa ctgctgagcg
      421 catacaaacg ctccggagaa agtaagtacg ccggtgaatt gttcaaacgc tacgcacacc
      481 tggttttggg cgtttgtatg aagtacctta aaaacatgga tgaagcgcag gataccacca
      541 tgtttgtgtt cgaaaaactg ctcagtgagt tgcgccaaac cgatgtgcaa cactttaaaa
      601 gctggttgta tatggtggca aaaaaccagt gcctcatgca tattcgcaaa caaaaacggc
      661 acgatattgt aaccttcgat accgaaaatg gtgaaaatga agatggcaat tctatggaaa
      721 ttggggcaat tgagcatctt gatgatgtag atttgaaaga agtgcaattg cagcaactgg
      781 aagaagggat taaaaccctt aaccatgagc agcaggaatg tatatcactt ttttaccttc
      841 aggataaaag ttatgtagaa gttgccgaaa ttaccggtta cgacctgaat aaagtaaaaa
      901 gttatattca aaatggtaaa cgcaatttga aaatctatct cgaaaaacga aatgtctgaa
      961 tttaaattac atatcgaaaa aggcggatac gtgagcaaaa caactttgct cgcttatttg
     1021 aacaatgaat taaatgcagc cgacaaacta tatgtggagc atttaatatc agaagaccct
     1081 tttttgcaag atgcaattga aggacttcgc ggtgctgata gtaaactggt tgaagcaaca
     1141 ttaaatgcga tatataaaga tgttgatgta attacaggaa ctaaaaaacc atttaccata
     1201 actgccacaa taaaaaaata tgcagcagct gccatgttga ttgtgttttt tggacttaca
     1261 tttttaatta tgaatgtgct caataaatca caaactaatg atgctatcgc aattgaaaac
     1321 gaagtagtgg aagcacaaca gtctgaagca aatggcaatg ccgacggcat gggcgcaggt
     1381 gatttgaata gtgatacatc aaacgaatct gaagtggcaa aaaccgtaag aagtaaattc
     1441 aataacgcca acgaaatacc aacaacttca aatccggaaa ttgtttacga caatagtagt
     1501 tccgttagtg aaggtgttag tgaaacaaaa tatttaaagg aagatatggt attagccgat
     1561 gaggaaatta ataccgataa agataaagta gtattatacg ataataaggt tgaagtaaaa
     1621 tcaacaacag ggaatgctca agttttaacc agtggtcctg tagtggtagg tgaagtatct
     1681 tataccgaaa gtgttacctt atcaactgta agcgaaaaaa aggttttaga caaagcaaaa
     1741 ccggcaagca ccgatgacgc ggattttggg tatatcgcag ctgattcaat agcaacagaa
     1801 ataaccaaac cggaatatcc cggaggttta gatgccttaa ataaatacct ggctaagtcg
     1861 atagattata aaaacaccgg tgaagtaaat ggtacaattt acgtccagtt tattattgat
     1921 gaaaacggga aaattaattc ccctgtaatt acaataggta taaacgaaaa agttaatgcc
     1981 gaagtaattc gctcaattaa tcgtatgcca aaatggaaac ctgcacatgt caataataaa
     2041 aatattaatg tccaaatttc tatgccgata tattttggtt ctcaaaatta atttttaatt
     2101 ttagggtacc atgaacccca aaattaaact gctttttgta ctatccattt ttatcatcag
     2161 tttatgtttg agtgcagact atattttctc tgcattttta acaaaaacaa ctcctgttgc
     2221 cgaatccaac aaaatttatc ggcttaccaa cgagcaacac aataacgaaa ttcccatatt
     2281 tggcagctca agagcggaaa aaggatatta ttgcgataac attaatccca attgttacaa
     2341 ttatggattt cccaatcaaa gttttaatgt aacagcactg ttgttaaatt tcgaactgaa
     2401 aaaaaataaa acaacaccga taattattga tattcatcac gacttttttc aacatgatgc
     2461 ctataccaat atcaatttag ctacctattt gccttttgtt aactccaacg atagtattac
     2521 tgctttctta gaacgaaaca agcgtttaaa gccttatcac tacgttccgg gcactagata
     2581 ttttggctat tataccacct atttagaacc catagttgaa aacaaaatga aaataggtaa
     2641 tgcattgtat ttaaagggtg gtgtatttga tactaaaaaa ggcaattcgc gaagtattca
     2701 aaaacgcatc gacaatagat tagataaaaa attggcgttt accgttactc ctgataacga
     2761 caaacaatta aaacaattaa taaccagtcg cccggacaga aaatttattt ttgtagtgag
     2821 tccttaccat tggagtgcaa aatcaactgt cgccaatttt gatgaaatgc taaattattt
     2881 taatggatta gctaaaaatt atccgaatgt acatttcatt tactttaata ctgataagta
     2941 cggcgacgaa ttatttaaag ataccgtaca tttaaatgaa gccggtgcca aacaattttc
     3001 tgctgaatta caaacgcatt tacaagcgtt agatatttat taatttcttg acgatatatc
     3061 ctcttctctt aataaacgct tgagtacttt tcctgaaggg ctctttggaa ttgatgaaac
     3121 aaaacgcaca acacttggca ctttaaaagg ggccagattt tttttacaat gttggataat
     3181 atcttcttcg tttgcggtga catctttttt aagta
//
LOCUS       DAORKW010000443         3214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1695_length_3214_cov_4.085470, whole genome shotgun sequence.
ACCESSION   DAORKW010000443 DAORKW010000000
VERSION     DAORKW010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1695_length_3214_cov_4.085470"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..2254)
                     /locus_tag="PLB46_13865"
     CDS             complement(59..2254)
                     /locus_tag="PLB46_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF77659.1"
                     /translation="MKKIGTIVFVLFAYVLQAQTDTNYNLDEVVISGSRFPDKRKEVA
                     QKIDVIKLEAISFSNSNTTADLLQQSSKVLVQKSQQGGGSPMIRGFEASRILLVVDGV
                     RLNNAIYRAGHLQNIITMDPSILAKAEIAYGPSSVVYGSDALGGVIHFHTRNPDLLSE
                     DENPFSGGAMLRYASAANSMAGNLHFNVASKKVASFTSVSYSDFDDLKVGSVENGDYG
                     NFNFRPYYVVTNNGVDALVKNDDKTIQVQSGYTQYDVLEKLLYQQNNKISHLLNFQYS
                     TSTDIPRYDRLTDPLNDDSLRSAQWYYGPQERLMAAYEFKYQSVNKLFDMAHLTASYQ
                     AIEESRHDRRYGRTTLNHRTENVGVIGFNFDFSKTVKKSNIRYGLESYLNDVSSVAEV
                     ENIEDGTFEPLDTRYPNGGSTMNNFAGYISHNLKFGNDKWMLNDGLRYNYSILNAKFT
                     DKTFFPFPYDEIDQKGGALTGSLGLIYSPDNATKLALNGSTGFRTPNVDDLTKVFESE
                     PGTIVVPNPDIKPEYTYNIDLNLVKVFADRVELELTGFYTIATNYFSTQPSTFGGEDS
                     IMYDGELSQVLTTVNSESAYLYGFNAGIDVKINDMFSFASYITYTYGRIKADSTPYPL
                     DHIPPMYGRTSFNFKTDKIHAELFALYNGWKRIEDYNIVGGEDNEQYATVDGMPAWYT
                     LNLKASYAIKDWLEVQAGCENILDLNYRVFASGIGAPGRNLFLALRTKF"
     gene            complement(2332..2787)
                     /locus_tag="PLB46_13870"
     CDS             complement(2332..2787)
                     /locus_tag="PLB46_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77660.1"
                     /translation="MLFTVLNTHNNMVINHKIDNDGFEDEELVNLKIAVNDPAFILIE
                     EDEFIYNDLLFDIIELKQENGWLQIKAYWDKKEEKLTADFIARTKSQHENKKDNQSNI
                     SIKQILKDYTGATDLHLTYFASTNVNGWYNEFLYLPEANHNIFIPPPEV"
     gene            2932..>3214
                     /locus_tag="PLB46_13875"
     CDS             2932..>3214
                     /locus_tag="PLB46_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77661.1"
                     /translation="MKKSTFIVLFALLSQSLSAQIIGGNGEGFGASFQDEDIRQITLL
                     STNSPIGVRWPGGGDSKVAFPAINKPGLGMHIDSVNALYDEFRDGQGYIK"
BASE COUNT          975 a          618 c          576 g         1045 t
ORIGIN      
        1 aattttgaaa ataagctcat attagtttgt ttgttggttc actgcgtaaa gttataaatt
       61 aaaatttggt acgcaaggcc aaaaacaggt tccttccggg tgccccaatg cccgaagcaa
      121 acaccctgta gttgaggtcc aatatgtttt cgcaaccggc ctgaacttcc aaccagtcct
      181 tgattgcata ggatgctttc aaattgagcg tgtaccatgc cggcatacca tcaacagtgg
      241 catattgctc gttatcctcc ccgccaacta tattgtaatc ttctatacgt ttccagccat
      301 tgtataaggc aaacaattca gcatggattt tatcggtttt aaagttgaag gaagttctgc
      361 catacattgg tggaatatgg tcgagcggat aaggtgttga atctgctttt atacgtccat
      421 aggtataggt aatgtaggaa gcgaatgaaa acatatcatt tattttgaca tctatcccag
      481 cattaaaacc atataaatag gcagattccg aatttactgt tgttaacacc tgacttaatt
      541 ctccatcata cataatggaa tcttcccctc caaaagttga tggttgggta gagaaataat
      601 tggttgcaat agtataaaat cctgtcagtt ccaattccac tctatcggca aatactttca
      661 ccaggttaag gtcgatattg taggtatatt ccggtttaat atcaggattt gggacaacta
      721 tagtacccgg ttcactttcg aatacttttg ttaaatcgtc aacattgggc gtcctgaaac
      781 ctgtggaacc atttaatgca agttttgtag cattatctgg tgagtaaatt aaccctagac
      841 ttccggtaag tgcgccacct ttttggtcaa tttcatcata cggaaaaggg aaaaatgttt
      901 tatcggtaaa ctttgcattc aatatggaat aattataccg aaggccgtcg tttaacatcc
      961 atttatcatt accaaatttc agattgtggg aaatataacc tgcaaaatta ttcatggttg
     1021 agccaccatt tggataacgt gtatctagtg gttcaaatgt accatcttcg atattttcta
     1081 cttcagctac agatgacaca tcgtttaaat aggactctaa tccgtatcgg atatttgatt
     1141 tttttactgt tttactaaaa tcaaaattaa atccgattac accaacattt tcggttctgt
     1201 gattaagtgt agtgcggccg taacggcggt cgtggcgact ttcttcaatg gcctgataac
     1261 tggcagttaa atgggccata tcaaacaatt tattcaccga ctggtattta aattcgtaag
     1321 cagccattaa tcgttcctgc ggaccgtaat accattgggc actgcgtaaa ctgtcgtcat
     1381 ttaacgggtc ggttaaacga tcataacgtg gaatgtcagt tgatgttgaa tattgaaaat
     1441 ttaataaatg ggatatttta ttattttgct gatacaacaa tttttcgagc acatcatatt
     1501 gcgtatatcc tgattgcacc tgaattgttt tatcgtcatt tttcactaat gcatccacac
     1561 cattattggt aacaacataa tatggtctga aattaaaatt cccatagtcg ccattttcaa
     1621 cactacctac ttttaaatcg tcaaagtcgg aataacttac tgaagtaaaa gatgccactt
     1681 tttttgaagc aacattaaaa tgtaaatttc ctgccatgga atttgctgca gaagcatatc
     1741 gcaacatggc gccgccgcta aatggatttt catcttcact taaaaggtca ggatttctag
     1801 tatggaaatg aattacaccc cccaatgcat cgctaccata aacaaccgat gatggtccgt
     1861 aggcaatttc tgctttggct aaaatggatg gatccattgt aatgatattc tgcaaatggc
     1921 ctgcgcgata aatggcatta tttaaacgaa caccatccac cactaacaaa attctgcttg
     1981 cttcaaatcc gcgaatcatt ggacttcctc caccctgctg acttttttgc actaacactt
     2041 tactactttg ttgtaaaagg tcggcagtgg tattactatt tgaaaatgaa attgcttcta
     2101 acttaataac atcaattttt tgagccacct ctttccgctt atccggaaaa cggcttcccg
     2161 atataaccac ttcatcgagg ttataatttg tatcggtttg tgcctgtaaa acgtacgcaa
     2221 acagcacaaa tactattgta cctatttttt tcataattaa ttttttgatg agtaaataat
     2281 aaacggcacc ttgagctgca aaaaaacaac caaggctgca aaaaaaatta attatacttc
     2341 gggaggtgga ataaaaatat tatgattggc ttcaggtaaa tataaaaact cattatacca
     2401 gccatttaca tttgttgaag caaaatacgt tagatgcaaa tctgtagcgc cggtataatc
     2461 ttttaaaatt tgtttaatac tgatattcga ctggttgtcc tttttgtttt cgtgctgact
     2521 ttttgttctg gcaataaaat cggcggttaa tttttcttct tttttatccc aataagcttt
     2581 tatttgcaac cagccatttt cttgtttgag ttcaataata tcaaacaaca aatcattata
     2641 aataaattca tcttcttcaa ttaaaataaa tgcggggtcg ttgactgcaa ttttgaggtt
     2701 caccaattct tcatcttcaa aaccatcgtt atcaatttta tggttaatta ccatgttatt
     2761 gtgggtattg agtaccgtaa ataacatata ggttcccgtt gaattaaaca ggaatgaaca
     2821 tagcacaatt attgtaatta actgtctcaa tcgtaatgtc tgatgcaaca aatatacaac
     2881 ctttgcaggc agattgcgta tttttgttgt tcaaaacccc aatttgaaat catgaaaaag
     2941 tcaactttta ttgttttgtt cgcgctactt agtcagtccc tttcagccca aattataggc
     3001 ggaaacggcg aaggatttgg cgcaagcttt caggatgaag atatacggca aataacctta
     3061 ttgtcaacaa acagtcctat aggcgtgcgc tggccgggtg gtggtgattc aaaagtggca
     3121 tttcctgcca taaacaaacc cggtttagga atgcatatcg attcggtaaa tgcactgtat
     3181 gatgaatttc gcgacgggca gggatacatc aagg
//
LOCUS       DAORKW010000444         3166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1726_length_3166_cov_4.118290, whole genome shotgun sequence.
ACCESSION   DAORKW010000444 DAORKW010000000
VERSION     DAORKW010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1726_length_3166_cov_4.118290"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3166
                     /locus_tag="PLB46_13880"
     CDS             <1..>3166
                     /locus_tag="PLB46_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77662.1"
                     /translation="FGGSAGLMPTIANGYYYTFNVTEYSTPGVPQNEYMGVVETSYNP
                     VAITSVIQSPAAGAVYPENSVYVTVTTAAIPSPGENVFVRYSTSVNFLTSSLLEVTMV
                     GTTGIVEIPCAGVGTTIYYYAYTSNRTGTAILTDVGVTGQRAHDMSTLNINNNGGPNY
                     SYTVSPSIGFCGDYYVPSVCYPTINSFVNALNSGSVSCDVVCYVAAGHTETAPVGGIN
                     LTQTGTAANTITFQKIGAGANPVINAYVGTITLGVASTVVDGVFSLNGSDYITIDGID
                     ILDNNASAPATMEYGYALYKASATDGCSYNTIKNCTITLSRLNTSTGPTLFQNGSRGI
                     FSANCTRTALGTSLIITTAGGRNDGNSFLANTISNVFSGIILRGYYDASPSTYFDQNN
                     TVGTAAEGNTITNYGGSSANAAYGVYGIYQSGLTVSGNTISNKTGGVSHTNTLYGIFH
                     SANTSTNSGSTTITYNDISLGQGANTNSVTGIRTGSSGQICGNVIIANNNIHDCTFDA
                     GASGQFYGIFQEFNTTSSNIDNNTFEKNNLNTTNANTYLIYDNGVTTTMSVDGNYMNN
                     NSVTTTGGVNLTGYYYINTSATGTINVANNIIDSLMIPSASTGYVIGIRVSNSTSQVK
                     TIASNTITNIRAGSGTTAWTCGMYIDKMPTGSSVTDNTVNNVSSAANVIGINCADDNS
                     INTSLNQIFTFTGNEVNNITSTSSSAQMAGIAIYALNAVDCGNNSVDSVIASGGSPIQ
                     LKGFNFGGGTVGNNMNFYGNTISNVKHDNGLGSATVVGLYLFPNTVTTNIYNNHIYEI
                     SGIGNDMIIAMYGTSGTGTHNIYNNFIQKLYANNTSLLLAVNGIYTAAGGNTYNVYNN
                     TIAIGQGGSISGGADFGVSGVYHGAGILNLRNNIIYLNVNTSGAGVSACVRKGTAGTA
                     GTAPAVTSIAATSNNNFYYINSNAYNYVYVEGGSTAAIKNGYAYGGATTSVTNNLNND
                     PCFNILTASDITSYKYFMSLSGGGSREINSFYDVPNFAGGAVIPDNLKITLGATDYAE
                     SHATSLALVTTDYEGEIRQGVVGYG"
BASE COUNT          989 a          579 c          624 g          974 t
ORIGIN      
        1 atttggaggc agtgcaggac ttatgccaac aattgccaat ggctattatt atacatttaa
       61 tgttaccgaa tatagcacac ccggagttcc tcaaaacgaa tatatgggcg ttgtggaaac
      121 tagttataac ccggtagcaa ttacttctgt aatccaatcg ccggctgcag gtgccgttta
      181 tcctgaaaat agtgtttatg ttacagttac caccgctgcc attccatcac ccggcgagaa
      241 tgtgtttgtg aggtattcta cctcagttaa ttttttgaca tcctcacttt tagaagtcac
      301 catggtaggc accaccggta tagtagaaat accttgtgca ggtgtcggaa caaccattta
      361 ttattacgca tacaccagca atagaaccgg taccgcaatt ttaaccgatg ttggggttac
      421 cggacaacgt gctcacgata tgagtacatt aaatattaac aacaatggcg gacctaatta
      481 ttcttatacc gtatcaccga gtatagggtt ttgtggcgat tattatgtgc cttcagtttg
      541 ttatccaaca attaattcat ttgttaatgc actcaattcc ggaagtgtaa gttgcgatgt
      601 agtatgttat gttgctgcag gtcataccga aacagctcct gtaggcggaa taaatttaac
      661 acaaaccggc actgctgcaa acactattac ttttcaaaaa attggtgctg gagccaatcc
      721 tgtaattaat gcatatgtag gaacaattac tttaggtgta gcttctacag tggtagatgg
      781 tgttttttct ttaaatggca gcgattatat caccatcgat ggtattgata tactagataa
      841 taatgcttcc gctcctgcga caatggaata tggttatgca ttatataaag catcagcaac
      901 tgacgggtgt tcatataaca caattaaaaa ttgcacgatt acattatcgc gtttaaatac
      961 atcaaccgga cctactttat tccaaaacgg aagcaggggg atttttagtg cgaattgtac
     1021 gcgaacagcg ctcggtacat cattgataat tacaaccgca ggtggacgaa atgatggtaa
     1081 tagttttttg gctaacacga tttctaatgt attttccggt attattttaa ggggctatta
     1141 tgacgccagc ccatctactt atttcgacca gaataatact gtaggtactg ccgcagaagg
     1201 aaacactata acaaattacg gtggttcaag tgcgaatgct gcctatggtg tgtatggcat
     1261 ttatcaaagt ggactaactg taagtggaaa tacaattagt aataaaaccg gtggtgtttc
     1321 acatactaat accttatacg gaattttcca ttctgccaac acaagtacta acagtgggtc
     1381 aacaacaatt acgtacaatg atatttcatt aggccagggt gccaatacca actcagtgac
     1441 aggtattcgc acaggctcat cgggacaaat ctgcggtaat gtgattattg caaataataa
     1501 tattcacgac tgcactttcg atgcaggtgc tagtggtcaa ttttacggta ttttccagga
     1561 atttaatact acctccagca atattgacaa caatactttt gaaaaaaata atttaaatac
     1621 taccaatgca aatacttatc tgatttatga taatggtgta actacaacca tgagtgttga
     1681 tggtaattat atgaataata attctgttac aacaacaggt ggcgttaatt taacaggtta
     1741 ttattatatc aataccagtg caacaggtac tatcaatgtt gccaacaata taattgactc
     1801 gctcatgatt ccatcagcta gtaccggtta cgtaattggc atcagggtaa gtaattcaac
     1861 cagtcaggta aaaacaattg catctaatac cattactaat attcgtgcag gctcgggaac
     1921 taccgcctgg acttgtggta tgtatatcga caaaatgcca acaggttcat cagttacaga
     1981 caatacggta aataatgtta gctcagcagc aaatgtcatc gggataaatt gtgcagatga
     2041 taattcaatt aatacatcgc tcaatcaaat atttaccttt acgggtaatg aagtaaataa
     2101 tattacctcc acttccagtt cagctcaaat ggctggaatt gctatttatg cattaaatgc
     2161 tgttgattgc ggcaacaata gtgtagatag tgtaattgca tcaggaggct ctcctataca
     2221 attgaaaggt tttaattttg gtggtggaac agttggcaat aacatgaatt tttatgggaa
     2281 caccatctct aatgtaaaac atgacaatgg gcttgggtca gcaacagttg taggtttata
     2341 tttattccct aacacggtta ccactaatat ttataataat catatttatg aaatttctgg
     2401 tattggcaat gatatgatta ttgccatgta tggaacatcc ggtaccggaa cgcataatat
     2461 ttataacaac tttatacaga aattatatgc taataatact tcattattat tagcagtaaa
     2521 tggtatttat actgctgccg gtggaaatac atacaacgtt tataataata cgatagcaat
     2581 tggtcaggga ggcagtattt caggtggtgc agattttggt gtttccggtg tttatcatgg
     2641 cgcaggaatt ttaaatttga gaaataatat catttacctg aatgtgaata catcaggtgc
     2701 cggtgtttct gcttgtgtga gaaaaggaac cgcaggtaca gcaggcacag ctccggcggt
     2761 aaccagtatt gcagctactt ctaataataa tttctattat atcaattcta atgcatataa
     2821 ttatgtgtat gtagaaggtg gatctacagc agccattaaa aatgggtatg cctatggcgg
     2881 agcaacaaca tcagtcacca acaatttaaa caacgaccct tgtttcaata ttttaactgc
     2941 aagcgatatc acttcgtata aatattttat gtcactcagt ggcggtggtt cacgtgaaat
     3001 aaacagtttt tatgatgtgc ccaattttgc cggtggtgct gtaatacccg ataatttaaa
     3061 aattaccctt ggtgccaccg attatgccga aagccatgct acatctttag cactcgtaac
     3121 taccgattat gaaggcgaaa ttcgtcaggg tgttgttgga tatggc
//
LOCUS       DAORKW010000445         3152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1741_length_3152_cov_4.035195, whole genome shotgun sequence.
ACCESSION   DAORKW010000445 DAORKW010000000
VERSION     DAORKW010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1741_length_3152_cov_4.035195"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PLB46_13885"
     CDS             <1..1111
                     /locus_tag="PLB46_13885"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRF77663.1"
                     /translation="AVTDDNRLRAALPTINKIINEGGAVVLMSHLGRPKTGPEDKYSL
                     RHVVQTLSVLLAKPVQFVNDCIGDVAVNAAKNLKAGEILLVENLRFHPEEEKGDELFA
                     QELAKLGDVYVNDAFGTAHRAHASTTIIAKYFDAAHKMFGFLMAAEVAAGTKVLHHHE
                     KPFTAIIGGAKVSDKIMILERLLEKADNIIIGGGMAYTFFKAMGGNIGSSLCETDKLD
                     TAKELLEKAKAKGVKILLPVDSILADKFDNAAQIQSGDSNNIPDGWMGLDIGEKAIQQ
                     FTDVIAASKTILWNGPMGVFEMENFSKGTITIAKAIAAATKNGAYSLVGGGDSVAAVN
                     KFHLADEVSYVSTGGGAMLEFFEGKVLPGVAAIEA"
     gene            1527..>3152
                     /locus_tag="PLB46_13890"
     CDS             1527..>3152
                     /locus_tag="PLB46_13890"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRF77664.1"
                     /translation="MNGYWNYIPLFRYLIPFIAGIIICLNFQFSQLWLNYLFYASALI
                     FTTLQLYPKTAASFSLQPFYGILLHLTLFLAGIVLVIHDTPIYRDNYFYHQADSTSIF
                     QCRITQPPEITTKNIRLYVDVTAHIKNNKQTPTIGKSILYIPLDSTNQHQLVYGDIIV
                     VKNGFRQPDPPKNPQAFNYQTYLYNQKIYYTAFLKQHEYQFSGINKGKPIWQFIFRIK
                     ANFIHNLETHLHDPDALAVAQTLIIGQKSVLDEEVKQAYANTGTMHILAVSGLHVGIL
                     FIILEVLFKPFSFFQKKQSKAALIKTILILIIIWMYTCLSGLSASVNRSAVMFTFLAI
                     GKTYERQTNTFNILFLSMLILLIDDPYQITQVGFQLSYIAVGGIVFFQPIITKLWYPK
                     NKLLQYIWGMTSVSLAAQLATSPISFFYFHQFPNYFLLSNIVAIPVSFVVLVLGLAFF
                     AMGTIPFIGNWIAFLLEWSLRIMNFTIIKIEQLPHALTTGLYLETPETIICYVALIYL
                     GAFLVLKEKRYLFKALISCLLVSGFITSRKISNN"
BASE COUNT          980 a          573 c          565 g         1034 t
ORIGIN      
        1 tgcagttacc gacgataatc gccttcgcgc agctttacct actataaata aaattattaa
       61 tgaaggtgga gcagtagtat taatgtcgca cctcggtcgc cctaagacag gacctgagga
      121 taaatattca ttgcgacatg ttgtacagac attatctgta ttattagcta aacctgttca
      181 atttgtgaac gattgtattg gagatgttgc tgtaaatgct gcaaaaaatt taaaagctgg
      241 agaaatttta ttggtggaaa atttgcgttt tcatcctgaa gaagaaaaag gtgacgaatt
      301 gtttgcacag gaattagcaa aattgggtga tgtatatgta aacgatgctt ttggaactgc
      361 ccaccgtgct cacgcttcaa ccaccataat tgctaaatat tttgatgcgg cacataaaat
      421 gttcggattt ttaatggctg cggaagtagc tgccgggaca aaagtgttgc atcatcacga
      481 aaaacctttt accgcaatta taggcggtgc aaaggtttcc gataaaatta tgattttgga
      541 acgcctgctc gaaaaagcag ataatattat catcggcggc ggaatggcct atactttttt
      601 taaagcaatg ggtggtaata tcggcagttc tttgtgtgaa acagataaat tagataccgc
      661 aaaagaatta ttggaaaaag cgaaggcaaa aggggtaaaa attttattgc ctgttgattc
      721 tattctcgca gataaatttg ataatgctgc acaaattcag tccggcgaca gtaataatat
      781 tcctgatggt tggatgggtt tagatattgg tgaaaaagct attcagcaat ttacggatgt
      841 tattgctgca tcaaaaacta ttttgtggaa cggacctatg ggtgtgttcg aaatggaaaa
      901 tttttcgaaa ggaacaataa caattgctaa agcgattgca gctgcgacaa aaaatggtgc
      961 ttattcatta gttggcggtg gcgatagtgt tgccgctgta aataaattcc atctcgctga
     1021 tgaagtgagt tatgtttcta caggtggtgg cgccatgctc gaattttttg aaggaaaagt
     1081 attacctggt gttgcggcta ttgaggctta ataaataatt gcgtattttt atatatcata
     1141 aatcgatgta ttggaataaa cgtttaaaaa gccgaaacca tggagggaaa tgcaaaggaa
     1201 acagcctaca aagtacacag catatatgaa ttaatattgg attatcattt gaattttgtg
     1261 tcacgatagc tatcgggatt gcgtgtattt aattttgctt tccggatcac tttaagtgtg
     1321 ggcaaaaaaa aactttattt tcactttgcg tactaccttt gtgttcactg tgcgcatttc
     1381 ctccgggttc tctttggttc cggctgttcc ccccccaaac tttgtaaccg cttacgatca
     1441 agtatatcaa gcactttgat tttcagctca ccatcgcgct tgtaatcctc aatacgacca
     1501 tttactttta actccgttta acccttatga acggatattg gaattacatc cctttattcc
     1561 ggtatttgat tccgtttatt gccggaatca tcatatgcct gaattttcaa tttagtcagt
     1621 tatggctgaa ttaccttttt tatgcaagcg cattaatatt taccacctta caactttatc
     1681 ctaaaactgc agcaagtttt tccttacaac ccttctacgg tattttatta cacctcaccc
     1741 tttttttagc aggaatagtt ttagttattc acgacacccc aatatatcgc gataattatt
     1801 tttatcatca ggcggacagc acatcaattt ttcaatgcag aataacacaa cctcccgaaa
     1861 ttaccactaa aaatatccga ctttatgttg atgtaactgc acatataaaa aacaacaaac
     1921 aaacaccaac cattggcaaa tccattttat atattccgct cgactcaacc aatcaacacc
     1981 aattagttta cggcgatatt atagttgtaa aaaacggatt tcgccaaccc gacccgccga
     2041 aaaatccaca ggcatttaat tatcaaacct atttatataa tcaaaaaata tattataccg
     2101 cttttttaaa acaacatgaa taccaattta gcggtatcaa taaaggtaaa cctatttggc
     2161 aatttatttt tcgcattaag gccaatttta tccataacct cgaaacacat ttacacgacc
     2221 ccgatgcctt ggctgttgcg caaacattaa tcatcggaca aaaaagtgtg ttggatgaag
     2281 aagtaaaaca ggcctacgcc aacacaggaa ccatgcatat tttagcagtt tcaggattac
     2341 acgtcggcat attatttatc atattggaag tgttatttaa accattttct tttttccaaa
     2401 aaaaacaaag taaggcagcg ctgatcaaaa caattttaat attaattatt atttggatgt
     2461 acacctgttt atccggatta tcagctagtg ttaaccgctc tgcagtgatg tttacttttt
     2521 tagcaattgg taaaacatat gagcgacaaa ccaatacgtt taatatttta tttttatcga
     2581 tgttgatttt gttaattgat gatccttatc aaattacaca agtaggattt caattgtcgt
     2641 acatcgccgt tggaggtatc gttttttttc aaccgattat caccaaatta tggtatccta
     2701 aaaataaatt attgcaatat atttggggaa tgacttccgt gtcactggca gcgcaattag
     2761 caacttctcc cattagtttt ttttattttc atcagtttcc aaattatttt ttgttatcca
     2821 acattgtagc cattccggtt tcatttgtgg tgttggtttt gggtttagca ttttttgcga
     2881 tgggaacaat tccctttatc ggcaactgga ttgcattttt attagaatgg agtttacgga
     2941 taatgaattt tacgataatt aaaatcgaac aattaccaca cgcattaaca acaggattat
     3001 atctggaaac acctgaaaca attatttgtt atgtcgcgct catttatctt ggagcgtttc
     3061 tggtactaaa agaaaaacga tatttattta aagctttgat ttcctgtcta ttggtaagtg
     3121 gattcataac atcccgcaaa attagcaaca ac
//
LOCUS       DAORKW010000446         3140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1748_length_3140_cov_3.744246, whole genome shotgun sequence.
ACCESSION   DAORKW010000446 DAORKW010000000
VERSION     DAORKW010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1748_length_3140_cov_3.744246"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLB46_13895"
     CDS             complement(<1..427)
                     /locus_tag="PLB46_13895"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF014857.2"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRF77665.1"
                     /translation="MKVLLLGSGGREHALALGLSKGRTLEPLYIAPGNPGTEKLGTNV
                     KLDIGDFHMVEEFVLELGINMIVVGPEAPLVAGIADHFKKHAHVQVIGPSAEGAQLEG
                     SKAFSKKFMQRHQIPTAGYFECDATNLQQGIDYLRTMGGP"
     gene            complement(658..1920)
                     /locus_tag="PLB46_13900"
     CDS             complement(658..1920)
                     /locus_tag="PLB46_13900"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77666.1"
                     /translation="MQLFGALAKMTLTFLCFIVSPYSFGQSLVPNGGFETYSALPFED
                     CDWDLATGWNNAATTDFCNTSNGTPDYYHILGTGFYSALLSNYFADVLPFEGDAFMGI
                     GGRVNLSPDSREYIAIPLTSPLVVGESYTMSYSITNGTPNVGCLFIDGWGASLSIGPI
                     LQTPGTNNVIPIADFDYFVPGVFSAEDWQTYTFTFTADQAYDTFTFGNFFTTASQTVT
                     TFGAVFFKVAYIFIDDISIKPITPLPIELVNFNVITEDNRTVKLIWETASEINNNFFT
                     IERSINAVDWEIVTTVDGAGNSNTLKNYVAKDNNPYLGVSYYRLKQTDFNGQFEYTSI
                     KNVTLKELYSESVEIYPNPTDSYITVVGSANELSEVRIFNVVGQDVTHLTYINSLNSS
                     TLSIDLSQLADGVYYLKTRTAINNMYKK"
     gene            2445..>3140
                     /locus_tag="PLB46_13905"
     CDS             2445..>3140
                     /locus_tag="PLB46_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRF77667.1"
                     /translation="MKIHSKSYFKDEIADLKYFENWVTQLEKNNNRQYERYDIETPLG
                     KTVVWGLNTKETYADTLVIFPGARTTSLIWDFDRGLDSFNHKMKIFMVETNGLPNLSN
                     GATPDIKSLDYGKWAAAILEQLNLEQAYVAGASFGGLICMKLAIVHPEKIKAAFLLNP
                     GCLQPFSLTLKNLYYNLLPIIKPTAKNVLKFLDNAVFSKPNHKLSDVSENMFIDYELL
                     ALKRYKDNTQKPYY"
BASE COUNT         1016 a          598 c          569 g          957 t
ORIGIN      
        1 aaggtccgcc cattgtcctt aaatagtcaa tgccctgctg cagattggta gcatcacact
       61 caaaataacc tgccgtaggt atctggtgcc gttgcataaa ctttttagaa aaggccttac
      121 tcccctccag ttgcgcccct tctgctgatg gtccgatcac ctgaacatgg gcatgcttct
      181 taaaatgatc ggcgatccct gctaccagtg gtgcttcagg acccaccaca atcatattaa
      241 tacctagttc taacacaaac tcctccacca tgtgaaagtc gcctatatcc aactttacat
      301 tggtacccag tttttccgta cccggatttc ccggggctat gtataatggc tccaaagtcc
      361 tgccctttga taagcccagg gccagggcat gttctctacc tccgctaccc aacaacaaaa
      421 ccttcattcc ataaaatttg cacaaaggta aggaaagtgg gtggacagag aaaattgaaa
      481 catttgagca attaattaac gttggtgctg gcaggcagtt tacggtttca aaaggccacc
      541 aatgcaaaac ccgaaactgt tggacccaat aaataccgca tcgaaattaa cttaaaagta
      601 aaaattagaa acatgattgc atgttatgtt gtgcattgaa ttaaaaaaca atttcaatta
      661 ttttttatac atattattga tggcagttct tgtttttaaa taataaacgc cgtctgctaa
      721 ttgcgataaa tctatcgata atgtggatga atttaaacta ttgatatagg ttaaatgagt
      781 aacatcttga ccaactacgt taaatatgcg aacttctgac aactcatttg cactgccaac
      841 aacagttata taactatcgg ttggattggg gtatatttca acggactctg aatataattc
      901 ctttagtgtt acatttttaa tggatgtata ttcaaactgt ccgttaaaat cggtttgctt
      961 cagtcgataa tacgaaaccc ccaaataagg gttattgtct tttgcaacat aattttttaa
     1021 ggtatttgag ttacccgctc catcaactgt tgttactatt tcccaatcca cagcgttaat
     1081 tgaacgctcg atggtaaaaa agttgttatt gatttcagaa gctgtttccc aaattaattt
     1141 aaccgttcta ttatcttctg ttattacatt aaaattaact aattcaatag gtaatggtgt
     1201 tattggttta atggagatat catcaataaa aatatatgcc actttaaaaa aaaccgcgcc
     1261 aaaagtggtt actgtttggc ttgctgttgt aaaaaaattc ccaaatgtaa atgtatcata
     1321 cgcttgatca gctgtaaacg taaaagtata ggtttgccaa tcctctgcgc taaatacacc
     1381 gggtacaaaa taatcgaagt ccgcaattgg tattacatta tttgtaccgg gtgtttgcaa
     1441 tataggccca atgctaagtg atgcgcccca accatcaata aaaaggcatc ccacatttgg
     1501 tgtgccattt gtaatcgaat aactcatagt ataactctca cccaccacta atggggaagt
     1561 gagcggaatg gcaatgtatt ctctgctgtc cggggataag ttaactctac ccccaattcc
     1621 cataaaagca tctccctcaa atggtaatac atcagcaaaa tagttggata ggagtgcaga
     1681 ataaaatccg gttcctaaaa tatgataata gtccggtgtg ccattcgacg tgttgcagaa
     1741 gtcggttgtt gcggcattat tccatcctgt tgcaagatcc cagtcgcaat cctcaaatgg
     1801 cagcgcactg taggtctcga atccaccgtt aggcacaagc gattgaccaa aagaataggg
     1861 ggaaacaatg aaacataaaa atgttaaggt cattttggct aatgccccga acaattgcat
     1921 ttttgtagga gttatattgt ggaaacagct caatttttac ttgtatttgt ttattttact
     1981 gcctggaaaa ttttatgata aaatcaaaaa tttgcccgta atatgttatt aataagcatt
     2041 aagcttaatt tatagttgtt aaacttgtct ttaaacaaaa ctattttgaa aaagtttatc
     2101 gggacaagag cacttttttt tggtgtgata gtttattggt aaagaagcag ttgacagcta
     2161 aactaagtat tatcctattt cactttggca gtttaaaagc cgtagtgcct ttatcaatca
     2221 ttaaacatgc gctataatgg taatatcggg ctgtcgtact cctgtcggga agctgaaatt
     2281 aaaaggtaaa cctgcatatt tataatatgc gggtttttta gctgtggagt cggatttaag
     2341 gttatataaa gtttgtatct tcgaacgagt aattgagtgg agcattcgct tatcgaaaat
     2401 ctgtcaaaca ttaaccctaa tgttaaaacc acacaaaaga aattatgaaa attcacagta
     2461 aatcttattt caaagacgaa attgcagacc tcaagtattt tgaaaattgg gtgacacaat
     2521 tagaaaaaaa taataacaga caatatgaac gttacgatat tgaaacccca cttggtaaaa
     2581 ctgtcgtttg ggggcttaac acaaaggaaa catacgctga cacgttagta atttttccgg
     2641 gagccagaac tacatcattg atttgggact ttgaccgagg tcttgacagc tttaatcata
     2701 aaatgaaaat ttttatggtt gaaacaaatg gacttccaaa tttaagtaac ggtgcaacac
     2761 cggacattaa atcactggac tatggtaaat gggcggcagc aattttagaa cagcttaacc
     2821 ttgaacaggc ttatgtcgca ggggcttcat ttggcgggct tatttgtatg aaactggcaa
     2881 ttgtacatcc ggaaaaaatt aaagcggcct ttttattaaa tcccggatgt ctccaacctt
     2941 tttccctgac cttgaaaaat ttatactata atctgttgcc aattataaaa cctacagcaa
     3001 aaaatgttct aaaatttctg gacaatgccg tattttcaaa acctaatcat aagttatcgg
     3061 atgtttcaga aaacatgttt atagattacg agctgcttgc actaaaacgt tacaaagaca
     3121 atacacaaaa accttattat
//
LOCUS       DAORKW010000447         3133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1757_length_3133_cov_5.442820, whole genome shotgun sequence.
ACCESSION   DAORKW010000447 DAORKW010000000
VERSION     DAORKW010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1757_length_3133_cov_5.442820"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..>1462
                     /locus_tag="PLB46_13910"
     CDS             123..>1462
                     /locus_tag="PLB46_13910"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HRF77668.1"
                     /translation="MKKLVLLFLAAAPLVAYNQCTVTNATDCHCLDGSDECDLLPDIT
                     LSHDQFEEPGNVIINPGLIQLGIGTPNIGHGPLRVISTDYYVCGLDTIYDPTGLETCP
                     DGTIPRQIINQRIYHKNGSEMSYWEKAAGSMTYHPDHGHFHTDNWGVYTLRKPIDGVE
                     DPTEWPILGYGTKMGFCLMDLANCASPGNYGYCREDDETVITNDIENYGLGGGNYSCA
                     ISNQGISVGYLDIYDWYLDGMEIVLPEGVCNGEYYIVVTVDPNLNYIEESDDNNVMAM
                     PFTLTGQPEEIDELNLAYTGGNELSPGVLTICASETVELNVPPIGIGYTWSNGATTNT
                     ITISEPGTYYCFVERECGDIYTDTIVVEMVESTLATISPAAPVCVGSATEINATGDGI
                     INWYSAAVGGTFLGTGATLLTTPLFENTMFYAENVTEIVNEVNAYVGQVNHEGS"
     assembly_gap    1463..1562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1563..2048
                     /locus_tag="PLB46_13915"
     CDS             <1563..2048
                     /locus_tag="PLB46_13915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77669.1"
                     /translation="TATFGDISPKLKRSTAGTNYPYNVDGLISITNSSFDESRWYYFY
                     DWHVTGNSVFACPSERTGVEVEVKVCTGIGEVSSLNAFNVYPNPNQGMFNVELNSSPV
                     DAVEVSVLNVTGQEVYSKLLNNVNGTVNIPVDLSDNAAGVYVVKITSNGNAVSKNVVV
                     E"
     gene            complement(2119..2940)
                     /locus_tag="PLB46_13920"
     CDS             complement(2119..2940)
                     /locus_tag="PLB46_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRF77670.1"
                     /translation="MKKLLLLLSFAAIIGTANAQKGAIDVTGMHIGLQGGFNSVWILN
                     QNNYGFQELEYARTFGFSTGLALGYNFKNNMGVQVDINYATMGQDYFDISKDFSNIDV
                     NGDNKAEKIETYRYVNLSYVQIPIQFRYQSIRTKKQIISFHAMAGPSLGFLAGADMYY
                     EADTAFNGNLVLIPGDTIDYMVPEFAQTAGPESADAYFSGFDLGFNLAIGADIYVTEQ
                     LYITPAAKFYYGLSDINSAPTRERRKDMDVPSYTGASKNAFGGLSVGIHYLLSAK"
BASE COUNT          965 a          503 c          584 g          981 t
ORIGIN      
        1 atattaattt tctgctaatt gccataataa tctcatactt ggagctgttt tttgcgggta
       61 tttaaaaaat tgttaacttt cgtcaccttt taaaagggca ttaacctcat caaaaaatta
      121 atatgaaaaa attagtactc cttttcctgg ctgctgctcc attggtggct tacaaccagt
      181 gtactgttac caatgctact gattgccatt gtttagacgg atctgatgaa tgtgatttgt
      241 tacccgacat taccctttcg cacgaccagt ttgaagaacc aggcaatgtg attattaacc
      301 ctggccttat tcaattgggt attggaactc caaatattgg tcacggtccg ctgcgcgtaa
      361 tatctaccga ttattatgta tgcggtttag ataccattta tgacccgaca ggattggaaa
      421 cttgccctga cggaacaatt cctagacaaa ttattaacca aaggatttat cataaaaatg
      481 gtagtgaaat gtcctattgg gaaaaggctg caggtagcat gacctatcac cctgaccacg
      541 gtcactttca taccgacaac tggggtgttt atacgttaag aaaaccaatt gatggtgttg
      601 aggaccctac agaatggcca attttaggtt acggtactaa gatgggattt tgtttaatgg
      661 atttagcgaa ctgcgcttca ccaggtaatt acggttattg tcgtgaagat gatgaaacag
      721 taatcacaaa cgatattgaa aattatggtt taggtggagg taattatagc tgcgcaattt
      781 ctaatcaagg tatatccgtt ggttatttgg atatttatga ctggtattta gacggtatgg
      841 aaattgtact tcctgaaggt gtttgtaatg gtgagtatta tatcgttgtt acagttgatc
      901 caaatttaaa ttatatagaa gaaagtgatg ataacaatgt gatggctatg ccatttacat
      961 taaccggaca acctgaagaa attgatgaat taaatttagc ttatacaggt ggcaatgaat
     1021 tatcaccggg tgtgttaact atttgtgcaa gcgaaactgt agaattaaat gttccgccaa
     1081 ttggtattgg ttatacatgg tcaaatggtg ctacaacaaa cacaattaca atttcagaac
     1141 ctggcacgta ttattgtttt gttgaacgtg aatgcggtga tatttatacc gatactattg
     1201 tagttgaaat ggtagaaagc acgttagcaa caattagtcc tgctgctccg gtttgtgttg
     1261 ggtcagctac agaaattaat gctacaggtg atggtataat taattggtat agtgcagcag
     1321 ttggtggtac ttttttagga acaggtgcaa ccttgcttac cactccactt tttgaaaata
     1381 ctatgtttta tgctgagaat gtaactgaaa ttgtcaacga ggtaaatgcg tatgttggtc
     1441 aggtaaatca tgaaggttct gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnacagcaac atttggcgat atttcaccta aattaaaacg tagtactgca ggaacaaact
     1621 atccatataa tgttgatggt ttaatttcaa tcaccaacag tagttttgac gaatcacgct
     1681 ggtattattt ttacgactgg catgtaacag gaaactctgt gtttgcttgc cctagcgaaa
     1741 gaacaggtgt tgaagttgaa gtgaaagtgt gtacaggtat tggagaagtt tcttcattaa
     1801 atgcatttaa tgtttacccg aatccaaatc agggtatgtt taatgttgaa ttaaattcat
     1861 cacctgttga tgcggttgaa gtaagtgtat taaatgtaac aggacaggaa gtgtattcca
     1921 aattgttaaa caatgtaaat ggaacagtaa atattccggt tgatttaagc gataatgctg
     1981 ccggtgttta tgttgtaaaa attaccagca atggtaatgc agttagcaaa aatgttgtgg
     2041 tagaataatt tatcacattt taaaaaataa aaaaaccgca gttgatttac tgcggttttt
     2101 tttatgccta ataaaatttt atttcgcact caataaataa tgtatcccaa cagataaacc
     2161 accaaatgca tttttagaag caccggtata agaaggaaca tccatatctt ttctgcgttc
     2221 acgtgtagga gcagaattaa tatctgataa tccataataa aatttagcag caggggtaat
     2281 gtaaagttgt tctgttacat aaatatctgc accaattgct aaattaaatc ctaaatcaaa
     2341 tccagaaaaa taagcatcag ctgattctgg tcctgccgtt tgcgcaaatt ccggaaccat
     2401 atagtcgatg gtatcacccg gaattaaaac caaattgcca ttaaaagctg tatcagcttc
     2461 gtaatacata tcagcaccgg ctaaaaatcc taatgaagga ccagccattg cgtggaaaga
     2521 aataatttgt tttttagttc ttatgctttg ataacggaat tgaataggaa tttgaacata
     2581 agataaattt acataacggt aagtttcaat tttttctgct ttattatcgc cattcacatc
     2641 aatattgctg aagtctttag agatatcaaa ataatcctga cccattgtag cataatttat
     2701 atctacctga acacccatgt tatttttaaa attataacca agtgctaatc cggttgaaaa
     2761 gccgaatgtt cgtgcgtatt ccaattcctg aaatccgtaa ttgttttgat tgagaatcca
     2821 tacgctgtta aaaccgccct gcaatcctat atgcattccg gtaacatcaa tagcgccttt
     2881 ttgtgcgttt gctgtgccaa ttatagcagc aaaactgagt agtaacaata atttcttcat
     2941 aattcgggtt ttaatcttca ctaccgcatt cgcggctttg ttaggtcaca aaaataatag
     3001 aaatcttttg tgaaatgcag tggattggta attcacccaa aaatgtgaaa tgaaattttt
     3061 aaaattgtga caagctaaaa gcttattttt ttaaccctat ttcacgtaaa cgttcgtcga
     3121 ggtattcacc agc
//
LOCUS       DAORKW010000448         3132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1758_length_3132_cov_9.805980, whole genome shotgun sequence.
ACCESSION   DAORKW010000448 DAORKW010000000
VERSION     DAORKW010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1758_length_3132_cov_9.805980"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PLB46_13925"
     CDS             complement(<1..192)
                     /locus_tag="PLB46_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77671.1"
                     /translation="MTKFSIKAYAASAISFLTIHQEADAQVVYVDIDPDIVLNEGGQA
                     AELDLDQNGTIDFWFHNNSF"
     gene            complement(189..2900)
                     /locus_tag="PLB46_13930"
     CDS             complement(189..2900)
                     /locus_tag="PLB46_13930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77672.1"
                     /translation="MNNPSYHNKHIWKFIYFIGALFLFSYLVGNSNIKNTVIANFIND
                     SIFSTDTIPPDTSVYEEEIIDDTLIFADGLDTIIELNDSTFTEDSLSDLMRDIFVTGP
                     QDILIKFGGVSKTINRGHFGIHVGGMFDNATLPNDGTSDYAWQWLIDLAPDVLRFPSG
                     SYSKFMHLLHNTDGTNATGYGYNIYEIARYFDWTDDTLEFNYYALDSIEIDHILNDTI
                     EQLDTWINPAHSEQFVKYRDKWFSQQCVTSRYIDDFIALVNQLDSAFPGRPSTKVILD
                     LNILSETATECRAIADYLRAHDVNVVGVEMGNETYADFFCDAMEFHNFEDYWSFINGS
                     NLSGNEYVLQDKPFTMEDMYSDHNFIHKFKTGGGYNYKIGVSGIPLEQGYAFKLDGDG
                     GETCAETAEWNSSLRSHYKEKISTKYKFDAVIMHTYYEVANWEDIPINGLAPITTCSG
                     TGDLWQFDTYDVRLRPAFDTIIGIGNKMGNFKWFLNRTDTIAFKASFNKFNDYFSFDL
                     PSDSAFKKELWVTEWNLKDNRNGLDNAADPTDDADPYKVEIYSNGITHGHLFFQWWLK
                     NIKINFDGAFRQNFFTYATVQNYAGGASTDLISTSDGIERDYYNKDNCPYDENCAAYN
                     CIYDDKFDKRNYHIRRTTYFVTYLLSTINKNNLKYLPSNYTMFLGNVNVQPTVFIDPN
                     KEYVYVFYDNMKDTIQNIILDPDNLKTFFPDKAYVDLDVATITYLQGKQLYSNSGKSA
                     LYDKEFINTCYATNDHPFEITPTTEAGLDSVIRTAINIPNCSGAGYINGCLSAPPYSI
                     GYFKLKLLPVEDPFRADNNASDYEIAIYPNPTSTVFTVKQYNANTITANADLDITIVS
                     MDGKVVFTTSTLANKPIFVSNLAAGCYQVIIKDGNNNLFYKKLIKTE"
BASE COUNT          975 a          574 c          560 g         1023 t
ORIGIN      
        1 aaacgaatta ttatgaaacc aaaaatcaat ggttccgttt tgatcgaggt cgagttctgc
       61 agcctgtccg ccttcattta atacaatatc agggtcgatg tcaacatata ccacctgcgc
      121 atcagcttcc tgatgtatag tcaggaagct gattgcagaa gcggcataag cttttataga
      181 aaatttggtc attctgtttt aatcaatttt ttataaaata aattgttgtt gccatcttta
      241 ataattacct gataacaacc tgctgctaaa ttagacacga atatcggttt atttgccaat
      301 gtgcttgtag taaatactac tttaccatcc atgctcacaa ttgtaatatc caaatcggca
      361 tttgctgtta ttgtatttgc attatactgt ttaacggtga atacagttga tgttggattt
      421 ggataaatag ctatttcgta atctgaggca ttattgtcag ccctaaatgg gtcttcaacc
      481 ggtaggagtt ttagtttaaa ataaccgata gaataaggag gagcagaaag acatccattt
      541 atataacccg cgccggagca attaggtatg ttaatagccg ttctaattac agaatctaat
      601 ccagcctctg tggttggcgt aatttcaaac ggatggtcgt tggtggcata acaggtatta
      661 ataaattcct tgtcatacaa tgcactttta ccggaattgg aatacaattg tttgccttgc
      721 aaataggtta ttgttgccac atctaaatca acataagctt tatcgggaaa aaatgttttc
      781 aggttgtccg ggtcaagtat gatgttttga atagtatctt tcatattatc ataaaacaca
      841 taaacatatt ccttgttcgg gtcgataaat acagttggtt gtacgttcac atttcctaaa
      901 aacatggtat agttggatgg caggtatttt aaattgtttt tattgatggt gcttaataaa
      961 taggttacaa aataagtagt tcggcgaata tggtaattgc gcttgtcaaa tttgtcatca
     1021 taaatacaat tatatgctgc gcaattttca tcgtaaggac aattgtcttt attataataa
     1081 tcccgctcaa tgccatctga ggttgaaatt agatcagtcg aggctccacc tgcataattc
     1141 tgaacggtgg cataagtaaa gaaattttga cggaatgcgc catcgaaatt gattttgatg
     1201 tttttaagcc accactgaaa gaataaatga ccatgggtta tgccattgga ataaatttca
     1261 actttatagg ggtctgcatc atcggttggg tctgctgcat tatccaatcc atttctatta
     1321 tccttcaggt tccattcggt aacccaaagt tcttttttaa aggcggaatc tgagggtaag
     1381 tcaaaactaa agtagtcgtt aaatttatta aaagaagctt taaatgcaat ggtatctgtc
     1441 ctgtttaaaa accatttgaa gttgcccatt ttgttgccta tgccaataat ggtatcaaat
     1501 gcaggtctta gtcttacatc gtatgtatca aactgccaca agtctcctgt gccactgcaa
     1561 gtcgttatag gtgctaatcc atttatagga atatcctccc aatttgccac ctcataatag
     1621 gtgtgcatga taactgcatc aaatttgtat ttagttgaaa ttttttcttt ataatgacta
     1681 cgcagcgaag agttccattc tgctgtctct gcgcaagtct caccaccatc accatctaat
     1741 ttaaatgcat acccttgctc aagcggaatg ccactaacac caattttata attataacca
     1801 ccacctgttt taaatttatg tataaaatta tggtcgctgt acatgtcttc catggtaaat
     1861 ggtttgtcct gcaacacata ttcatttccg gataaattac tgccattaat gaacgaccaa
     1921 taatcttcaa aattgtgaaa ctccatagca tcacaaaaaa aatcggcata ggtctcatta
     1981 cccatttcaa caccaacaac atttacatca tgtgctctca ggtaatcggc aattgctcgg
     2041 cattcggttg ccgtttcgct taaaatattg aggtctaaaa taacttttgt ggatggtctg
     2101 ccaggaaaag cagaatccaa ttgatttact aatgcaataa aatcatcaat atatcgcgag
     2161 gtgacacatt gttgagaaaa ccatttgtca cggtatttaa caaattgttc agaatgagca
     2221 ggatttatcc aagtatctaa ttgttctatt gtgtcattta aaatatgatc aatttcaatg
     2281 gaatcaagtg cataataatt aaattccagc gtatcatctg tccagtcaaa ataacgtgca
     2341 atttcataaa tattataacc atagccggta gcattagtgc catcagtgtt atgcaataaa
     2401 tgcatgaatt tactgtatga accactcggg aaacgcaaca catcgggggc aaggtcaatt
     2461 agccattgcc aagcataatc gcttgttcca tcattcggca atgtagcatt gtcaaacata
     2521 ccgcccacat gtataccgaa atgtccgcgg tttatcgttt ttgatacacc gccaaattta
     2581 attaatatgt cctgaggacc ggtaacaaaa atgtcccgca ttaaatcact taaactgtct
     2641 tcggtaaagg ttgagtcgtt tagttcaatg atagtgtcta ggccgtccgc aaaaatgagt
     2701 gtatcgtcta taatttcctc ttcataaact gatgtatccg gtggaattgt gtcagtggaa
     2761 aatatggaat catttatgaa atttgctatt acagtatttt taatattgct gttaccaact
     2821 aaataggaaa acaaaaacaa tgctccaatg aagtaaataa acttccaaat gtgtttatta
     2881 tgataagaag ggttattcat ttgaattgat tttaaagtta attaaaaaat ggtttggggt
     2941 aaaattttaa ccaaaatagc tttaaaacct ttgtcttcgc ctgcgcttat tcagtctgtc
     3001 aaccgaccag atggtgaatt gctcaccccg ttttttggaa cggtgaattt catagaccat
     3061 atcctataac gttttgccgc tttgcgaagg cggggatttt cagcactaaa cttcattaga
     3121 tgcacaaagc tt
//
LOCUS       DAORKW010000449         3114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1772_length_3114_cov_4.875776, whole genome shotgun sequence.
ACCESSION   DAORKW010000449 DAORKW010000000
VERSION     DAORKW010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1772_length_3114_cov_4.875776"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3114
                     /locus_tag="PLB46_13935"
     CDS             <1..>3114
                     /locus_tag="PLB46_13935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRF77673.1"
                     /translation="AMMGATFGDDDEEFMYIQDYSSYAMTIVFKTETTIGDITLCTVT
                     LEIPGDDAALTLEDISENIVLIAHEELGVGGTTHEFTLECNDDVLCGEDDLAITVWGH
                     IDCIGLKNCPCENNLVDDYFFNSQTVDAQIGNGGGIGEIEEGEIETTPGTSTNCDLDV
                     IYTPMLPDIDPCLADQMAVALNNAYAAYNNQVATYINNFEAGYTAHCLENLRETLVKS
                     YAITEYHYTLYYYDQAGNLTMTVPPAGVDVLTGSSLDSMANYRKKLALAEPAKPQHNL
                     ATSYWYNSLSQLCKQKTPDAGISKFWYDYLGRIVLSQNAEQANIVEDEWKFSYTLYDG
                     LGRIYEVGEITLDYDAGWVADVAEALESESNLFAFVSAAERAQVTSTYYDTEFDLAGD
                     PFGAEGQKNLRNRIGAVTIEVDFDNIAATYDHATHYSYDIHGNLFTLVQENPAMGEIQ
                     AEEQFKRIDYTYDLISGNVHEVAFQHDKADQFYQHYDYDADNRIYEVQTSRDGWIWEK
                     EARYFYYRHGPLARTEIGDLIVQGNDYAYTIKGWLKGVNAGALIADRDMGKDGYDDAS
                     NSHKYIAQDAFGFILDYFESDYSRINSGAVYKFIPNYTATNYNGVANSLYNGNIMSMS
                     TALLQPLTETDADGHHDKANVLGKIYAYDQLNRIASSNSYEKAMTSTSNFTWADTDGP
                     LEPTYASQNWNTTYTYDGNGNILSMQRAGNQNTAYDMDQLTYHYIDNTNQLLYVDDAI
                     TDDGAYDVDVDDQEIYNYTYDRIGNLTADVAEGIEKITWNVYGKIKKIVKENESGLVF
                     EYDAIGNRISKTVIPVTGDVTTTYYVRDAMGNVMAVYTHTASDTATFVITEQHIYGSS
                     RIGMVQTNIDMLVTFTEDNYFYRILGEKRYELSNHLGNVLSVISDRRLAFDSDANGTT
                     NYYEADVMSSMDYDPYGMLLTGRNWEEVSEPRYGFNGKESDTETYGIGNIYDYGFRIY
                     NPRLGKFLSIDPLYKFYSQLTPYQFASNTPICAVDIDGQESKIVIREVEKNADGTYKI
                     VSVRTII"
BASE COUNT         1040 a          494 c          661 g          919 t
ORIGIN      
        1 tgcaatgatg ggtgctactt ttggcgatga tgatgaggaa tttatgtaca tacaagatta
       61 ttcttcttat gcgatgacca tcgtttttaa aacagaaact accattggag atataacatt
      121 atgcacggta acattggaaa taccaggtga tgatgctgca ttgacacttg aagatatcag
      181 cgaaaatatt gtattaattg ctcacgaaga attaggcgtt ggaggcacta ctcatgaatt
      241 taccttggaa tgtaacgatg atgttttgtg cggagaagat gatttagcaa ttactgtttg
      301 ggggcatatt gattgtatag gtttgaagaa ttgtccttgt gaaaataatt tagtagatga
      361 ttattttttt aatagtcaga ctgttgatgc acaaattggc aatggtggag gtattggtga
      421 aatagaagaa ggtgaaatcg aaactacacc tggtacatcc acaaattgtg atttagatgt
      481 gatttataca ccaatgttgc cggatattga cccatgtttg gccgaccaga tggcagttgc
      541 cttaaataat gcctatgccg cttataataa tcaggtggca acctatatca ataatttcga
      601 agctggttat acagcacatt gtttagaaaa tttacgtgaa acactagtta aaagttatgc
      661 gataaccgaa tatcattata cattatatta ttatgatcag gcaggtaatt taaccatgac
      721 tgttcctcct gctggtgtgg atgtactaac cggatcttcg ttggattcta tggctaatta
      781 tcggaaaaaa ttagctttag ctgaacctgc taaacctcaa cataatttag caacatctta
      841 ttggtataat tcactgagtc aactatgtaa acaaaaaaca ccggatgcag gaatcagtaa
      901 attctggtac gattaccttg gccgtattgt attgtcacaa aatgctgagc aggcaaatat
      961 tgttgaagat gaatggaaat ttagttatac gttatatgac ggattaggtc gcatttatga
     1021 agttggtgaa ataaccttgg attatgatgc cggatgggta gctgatgtag cggaagcatt
     1081 agaatcagaa agtaatttat ttgcttttgt aagtgctgct gaacgtgccc aggttacttc
     1141 tacttattat gatacagaat tcgatttggc gggcgaccca tttggtgcag aagggcaaaa
     1201 aaatctgcgt aacaggattg gagcagttac aatcgaagta gattttgaca acattgcagc
     1261 aacatatgac catgccacgc attatagtta cgatattcat ggcaatttat ttaccctcgt
     1321 acaagaaaat cccgcaatgg gcgaaattca agctgaagag caatttaagc gtattgatta
     1381 tacctacgat ttaataagtg gaaatgtgca tgaagtagct tttcaacacg ataaagccga
     1441 ccagttttac cagcattatg attacgacgc cgacaataga atttatgaag tgcaaacttc
     1501 acgcgacggc tggatttggg aaaaagaggc aaggtacttt tattaccgcc atggaccttt
     1561 ggcaaggaca gaaattggtg atttaattgt acaaggtaat gattacgctt acacaataaa
     1621 ggggtggcta aaaggggtaa atgccggagc attaattgca gaccgcgata tgggtaaaga
     1681 tggatatgat gatgcaagta atagtcataa atatattgcg caagatgcat ttggttttat
     1741 tttggactat tttgaaagcg actattcacg tatcaatagt ggggctgtct ataaatttat
     1801 tccaaactat acggctacaa actataatgg tgttgccaat agtttataca atggtaatat
     1861 tatgtcaatg tctactgcac tattacaacc tttaactgaa acagatgcgg atgggcacca
     1921 tgacaaggct aatgttttgg gtaaaattta tgcttacgac caattaaaca gaattgcaag
     1981 tagcaacagt tatgaaaagg caatgacaag cacttccaat tttacttggg ctgatactga
     2041 tggacctttg gagccaacat atgcttctca aaattggaat accacataca cctacgatgg
     2101 aaatggaaat atcctttcca tgcaaagagc aggtaaccaa aacactgctt atgatatgga
     2161 ccagttaacc tatcactaca tagataatac caatcagctt ttgtatgtag atgatgctat
     2221 tactgatgac ggagcatatg atgttgatgt ggatgaccaa gaaatatata attacacata
     2281 cgacagaatt ggcaatttaa ctgccgatgt tgcagaagga atagaaaaaa tcacttggaa
     2341 tgtatatgga aaaattaaaa aaatagtaaa agaaaatgag tccgggcttg tttttgaata
     2401 tgacgccatt ggaaatcgaa taagtaaaac ggtaataccg gtaaccggtg atgtaacaac
     2461 cacctattat gtgcgcgatg caatgggcaa cgtgatggcg gtgtataccc ataccgctag
     2521 tgacacagca acatttgtaa tcacagaaca acatatttac ggcagtagca gaattggtat
     2581 ggtgcaaacc aatatcgaca tgcttgttac cttcacagaa gataactatt tttaccgcat
     2641 cttaggtgaa aaacgatatg aactatctaa tcaccttggt aatgttcttt cagttattag
     2701 cgaccgccga ttggctttcg attcagatgc aaatggaact accaattatt atgaagcaga
     2761 cgtgatgtct tcaatggatt atgatcctta tggaatgttg ctgacaggta gaaattggga
     2821 agaagtaagc gagccaaggt atggatttaa tggtaaggaa agtgacacag aaacatatgg
     2881 tataggaaat atttatgatt atgggtttag gatatacaac cctagacttg ggaaattttt
     2941 aagcattgat ccactatata aattctattc ccaattgact ccttatcaat ttgcaagcaa
     3001 tacacctatt tgcgcggtag atattgatgg tcaagaaagt aaaattgtaa taagggaagt
     3061 ggaaaaaaat gctgatggta cctataaaat tgtttctgtt agaaccatta ttca
//
LOCUS       DAORKW010000450         3104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1777_length_3104_cov_4.797967, whole genome shotgun sequence.
ACCESSION   DAORKW010000450 DAORKW010000000
VERSION     DAORKW010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1777_length_3104_cov_4.797967"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLB46_13940"
     CDS             <1..263
                     /locus_tag="PLB46_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HRF77674.1"
                     /translation="NDVELFDENALEKLAADDLLQMIASLSPGYRTVFSLYAIEGFSH
                     KEIAEQLNISEGTSKSQLARARYILIEMVEQRTGIKRKASGQ"
     gene            253..1476
                     /locus_tag="PLB46_13945"
     CDS             253..1476
                     /locus_tag="PLB46_13945"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF77675.1"
                     /translation="MDNKQHIDQLFKSVVENYELKPSADVWNRIEEDLNQNKTTHTSA
                     SLVRYAAILLVIIAVTLFALPISKNTPISLQGNAIDQNQPIVALSPSNSTSAIITTDN
                     NTVVAQVPVTTNRNIIDDTQQSNLNTFASNTIPFHNQESDFIFMPQETEDESLAAANR
                     DIIDRIYNNDQSIDVFAFGELASYEDDDEMQLIQPDNAIAYKDYSMRGVYIGASGSYN
                     QTSIIEYGNTFKGARPIQPSLKFGSSKGLMLGYNFNNKFGIEAEFIYNAIQGQNYVMS
                     EDEQIVEKSLSLNYDLIPVVAKIKVGRISNITNLPVVLNYTAGIQYGMLREARLPQDK
                     RYEETTEELFTDQDLSVVLGLEYDIFMQENLMLTLGTRGAISTDISTHSGPLSYYNKR
                     NFVFGLRAGLSYAFN"
     gene            1529..>3104
                     /locus_tag="PLB46_13950"
     CDS             1529..>3104
                     /locus_tag="PLB46_13950"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HRF77676.1"
                     /translation="MIRLLTFLLLVSYSSVKVNATVTIKGTVTDESDQPLPFCNIYIP
                     NTSYACISNETGNFVLQVEPGKYTITFQFIGFKKQNIPVHAVTEAITLTIKMEPEVLE
                     LQEVVINSDKEDPAYAIIRKAIEYRQDNLQSVRSFSCDAYIKGLQKLIEAPDKVLGIQ
                     LNTIVDVDSNNSGIVYLSESSSTFHYQYPDKSKEIMHASIVSGDDQQFSWNDAASMQM
                     NFYNNLETLEGFSQRGFVSPIADNALFFYTYSLIGQTYENNHIIYKIAVAPKRKSDPA
                     YTGTIYITDHEYRFTALQLELTSLNGIEFIDTFTVSQEFFYTDPDHLVLRSNQFNFNY
                     NFFGIKGMGYFHASYNNYTLNAIYPKNFFNGEVIKIEANANKKDDTYWSNIRPIQLTT
                     EEQKDYIAKDSLAIIKETTAYKDSMDRIFNKLDAADIITGYTWRDSDKKIVASSNPLP
                     DMLHFNTVEGYVVNPKIRLTKWFENKNEINFYPGVRYGFNSDTWYANAGLSYKFDPLH
                     LANIAISGGTGVQQFNT"
BASE COUNT         1055 a          557 c          544 g          948 t
ORIGIN      
        1 ataacgatgt agagttgttt gatgaaaatg ccctggaaaa gcttgctgca gacgatttgc
       61 tgcaaatgat tgcaagttta tctcccggat atagaacagt ttttagctta tacgcaatag
      121 aaggattttc acataaagag atagccgagc aactaaacat cagtgaagga acgtctaaga
      181 gtcaattagc acgtgcaagg tatatcttaa tagaaatggt agaacaacgc actggcataa
      241 aacgcaaagc aagtggacaa taaacaacat attgaccaat tatttaaaag tgttgttgaa
      301 aattacgaat taaagccttc tgccgatgtc tggaaccgca tcgaagaaga tctgaaccaa
      361 aacaaaacaa cacacacatc cgcctctttg gtgcggtatg ctgctatatt attggtaatt
      421 attgctgtta ccttgtttgc cctcccgatt tcaaaaaata caccaatcag tttacagggc
      481 aatgcaattg accaaaatca acctattgta gcactttcac caagtaattc cacttcggct
      541 ataataacta ctgataataa caccgttgta gctcaagttc ctgtaaccac aaatcgcaat
      601 attattgatg atacgcagca atctaatcta aatacattcg ctagtaatac catcccattc
      661 cataatcagg agtccgattt catttttatg cctcaggaaa cagaagatga aagcctggcc
      721 gcagctaacc gcgatataat tgaccgaatc tacaataatg atcaaagtat tgatgttttt
      781 gcttttggag aacttgccag ttatgaggat gatgatgaaa tgcaattaat tcaacctgat
      841 aacgccattg cttataaaga ctacagtatg cgtggcgttt atattggtgc cagtggaagt
      901 tataatcaaa cttccattat tgaatatggt aatactttca aaggtgcacg tccaattcaa
      961 ccttcattaa aatttggctc atcaaaaggt ttgatgttgg gatataattt taataacaaa
     1021 ttcggtattg aagctgaatt tatttacaac gctattcaag gccaaaatta tgtgatgtca
     1081 gaagacgaac agatagttga aaaatcactt tctttaaatt atgatttaat tccggtagta
     1141 gcaaaaataa aagttggtcg tatttcaaat atcaccaatt tacctgttgt gttaaattac
     1201 accgccggaa ttcaatatgg tatgttaaga gaagctcgtt tgccacagga taaacgttac
     1261 gaagaaacta ctgaagaact atttacagat caggatttat ccgttgtatt aggtttggaa
     1321 tatgatattt ttatgcagga aaatctcatg ttaacgctcg gaaccagagg tgctatttct
     1381 actgacatct caacacactc cggcccactc agctattaca ataaacgcaa ctttgtattt
     1441 ggattgcgag cagggttgag ttatgccttt aattgattat tacaacaatt ttccttatta
     1501 agcgttagtc tgtattatct ttaccctgat gatacgattg ttgacttttt tactgctggt
     1561 tagttatagt agtgttaagg taaacgcaac agttactata aaaggaacag ttaccgatga
     1621 gtctgatcaa ccactcccct tttgtaatat ttatattcct aatacttctt atgcctgtat
     1681 aagcaatgaa accggaaatt ttgtattaca ggtagagccc ggcaaataca caattacttt
     1741 ccaatttatt ggtttcaaaa aacaaaatat tcctgttcat gcagtaactg aagcaattac
     1801 tttaactatc aaaatggaac ctgaagtgtt agaattgcaa gaagtagtga ttaattcgga
     1861 caaagaagac cctgcgtatg ctatcattcg aaaggccatt gaatacagac aggataattt
     1921 acaatcagtt cgttcattta gttgtgatgc ttatattaaa ggtctgcaaa aattaattga
     1981 agctcccgat aaggttttag gaattcaatt aaataccatt gttgatgttg attcaaataa
     2041 ttcaggaatt gtgtatttat ctgaatcttc ttctaccttt cactatcaat atcccgacaa
     2101 atcgaaagaa attatgcacg ccagcattgt aagtggcgac gatcaacaat ttagctggaa
     2161 cgatgcagcc agtatgcaaa tgaattttta taataacctc gaaacattag aaggtttttc
     2221 acaaagagga tttgtttctc caattgcaga taacgccttg tttttttata cctatagtct
     2281 gataggtcaa acctatgaaa acaaccatat tatttacaaa atagcggtag caccaaaacg
     2341 taaatcagac cctgcttata ccggcacaat ttacatcacc gatcatgaat atcgatttac
     2401 ggctttgcaa ctagagctta cctctttaaa tggcattgaa ttcatcgaca cctttactgt
     2461 ttctcaggaa tttttttaca ctgatcctga tcaccttgtg ctaaggtcaa atcaattcaa
     2521 tttcaattat aattttttcg gaataaaggg aatgggatat ttccatgcat cttataataa
     2581 ttatacacta aatgccatct acccaaaaaa cttttttaat ggtgaagtaa ttaaaataga
     2641 agcaaatgcg aataaaaaag atgatacgta ttggagcaat attcgtccca tacaattaac
     2701 tactgaagaa caaaaagatt atatagctaa agattcgttg gcgataatca aagagacaac
     2761 tgcctataag gattctatgg accggatttt caataaatta gatgcggcag atattataac
     2821 cgggtatacc tggcgcgaca gcgataaaaa aatagtagcc agctctaatc ctttgccgga
     2881 catgttacat tttaatactg tagaaggtta tgtagtgaat ccaaaaattc ggttgacaaa
     2941 gtggttcgaa aataaaaatg aaataaattt ttaccccggt gtaagatatg gatttaacag
     3001 tgacacctgg tatgccaatg cgggtttaag ttataaattc gatccgttgc acctcgcgaa
     3061 catcgcaatt tcaggtggaa ccggtgtgca acaatttaat actc
//
LOCUS       DAORKW010000451         3090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1795_length_3090_cov_6.054366, whole genome shotgun sequence.
ACCESSION   DAORKW010000451 DAORKW010000000
VERSION     DAORKW010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1795_length_3090_cov_6.054366"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2834)
                     /locus_tag="PLB46_13955"
     CDS             complement(<1..2834)
                     /locus_tag="PLB46_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRF77677.1"
                     /translation="MYIKSLFILLFTVGFASASFNTQLPENDVEHKIPFYDQNYPWVD
                     SLMATMTLDQKIGQLFMVAAYSNKDAAHVKEIETLIKKYQIGGLIFMQGGPVRQVKLT
                     NNYQSMSNIPLMIAMDAEWGPAMRLDSVISFQRQLTWGAMTHDSTVYEVGTIIAQQLK
                     RLGVHANFAPDIDINNNYKNPVIGDRAFGEDKYNVALKGLMYMNALQDNNILACGKHF
                     PGHGDVDADSHVTMPVVNHSYAHLDSLEFYPFKILMNEGLGSVMLAHLFVPALDNVEN
                     SAASISQKIGTGVLRDSLGFRGLVFSDALNMKGISAYYKSGELEVKAFLAGNDFLLFS
                     ENIPVAFEAIKAAVASGKISEQRLDESVSRILKAKAFAGLNDYKPVKVQNLTADLNAD
                     EATLVKRKITEHSITLANAIDSLIPFKKLDTLSIASVSVGNGSKSTFQEYLSKYGKVD
                     NYQINKDADAAAYIQIYEKLKKYDVVIVGVHDMKRARSSNYGVTSQTENLVWKLSLVT
                     KTATVLFGIPYALEKFQDAQYHIVAYDDDKYTQQAVAMSLFGAIPFSGRLPVGIGEFK
                     VNDGMLTGSLNRLKYTIPEELGISSLRMSRIDSLAKACIQVEAAPGCQILVAKDGKII
                     WDKAYGYYKYDKKTPVENSSIYDLASVTKTSATTMMLMQLHEQGLLDLTKTVADYLPD
                     TKGSNIAGLKLQDILTHQSGLPAWVPFYKKTLLADGTQDPGYYSQTKIAGYTTQVADN
                     VYMRDDYRDTVWQIIKDINLKEKNKYVYSDLGLYISRRIAEQLTGVRLDNYVYDHFYN
                     SLGMTTTCFNPLNLFPRTQIVPTENDTYFRYQTVQGYVHDMGAGMMGGVEGHAGLFSN
                     AEDLVKLYQMLLNGGTYGGERYFKEETVNLWTKKQSSISRRGLGFDKPDMENVSPCSG
                     YASAATFGHQGFTGICVWVDPKYDLVYIFL"
BASE COUNT          959 a          617 c          504 g         1010 t
ORIGIN      
        1 ctaagaaaaa tatatactaa atcatattta gggtccaccc aaacacaaat tcctgtaaac
       61 ccctgatgac cgaaagttgc ggcagaagca taaccactgc aaggagaaac attttccata
      121 tccggtttat cgaatcccaa acctcttctt gaaatactac tttgtttttt tgtccagaga
      181 tttactgttt cttctttaaa atatctttca cctccgtagg tgccaccgtt taataacatc
      241 tgatataact taaccaggtc ttctgcattt gaaaataaac ctgcatgacc ttctacacca
      301 cccatcattc cggcacccat atcgtgcaca taaccctgaa cggtttggta acggaaataa
      361 gtatcatttt ctgtgggaac aatttgtgta cgtggaaata aatttaaagg attaaaacaa
      421 gtagttgtca tgccaagcga attataaaaa tggtcataca cataattatc caatcgcact
      481 cctgtcagtt gttcggcaat tcttcttgaa atatataatc ctaaatcgct ataaacgtat
      541 ttattttttt ctttgaggtt gatgtctttt attatttgcc aaactgtatc acgataatca
      601 tcacgcatat aaacattatc agcaacctga gtggtataac ctgcaatttt tgtctgactg
      661 taataaccgg ggtcttgtgt tccatcggct aatagagttt ttttataaaa cggcacccag
      721 gcaggcaaac cggattgatg tgttaaaata tcctgcaatt ttaatcctgc aatattggac
      781 cctttggtat cgggtaaata atcggcgact gtttttgtta aatcgagcaa accctgttcg
      841 tgcaattgca tcaacatcat agtagttgca gaagttttgg taacactcgc taaatcgtaa
      901 atactagaat tttcaacagg tgttttttta tcgtatttat aataaccata agctttatcc
      961 caaattattt tgccgtcttt ggctactaaa atctgacaac caggagcagc ttctacctga
     1021 atacaagctt ttgcaagact atcaattcgt gacatcctta aagagctaat tcccaactcc
     1081 tccggaatgg tatattttaa tctgtttaaa gaaccggtaa gcataccatc atttacttta
     1141 aattcaccaa ttccaactgg gagcctgcca ctaaaaggaa tagcaccaaa taaactcata
     1201 gcaacagctt gctgggtata tttatcatcg tcgtaggcaa caatatgata ttgtgcatct
     1261 tgaaattttt ctaaggcata aggaatacca aataatacgg tagctgtttt agttaccagt
     1321 gataatttcc aaacaagatt ttctgtttgt gaggtaacac cataattact tgagcgtgcg
     1381 cgtttcatat catgcacacc cacaataacc acatcgtatt tttttagttt ttcatatatc
     1441 tgaatgtagg cagcggcatc ggcatcttta tttatttggt aattatctac cttgccatat
     1501 ttacttaaat attcctggaa agttgattta gaaccattgc ctacacttac acttgcgatt
     1561 gataaagtat ctaatttttt aaatggaatt aaactatcaa ttgcattagc caatgttata
     1621 ctatgttcgg taatttttct ttttactaag gtcgcttcat ctgcatttaa atcggcagtt
     1681 aagttttgta ctttaactgg tttgtaatca tttaaaccgg caaatgcttt cgcttttaaa
     1741 attctgctta cactttcatc taatcgctgt tcagaaattt ttccagaggc aacagcagct
     1801 tttatagctt cgaaagcaac aggaatattt tcgctgaaca acagaaaatc gtttcctgct
     1861 aaaaatgctt ttacttccag ttcaccactt ttgtaatacg cactaattcc tttcatgttt
     1921 aatgcatcac taaataccaa acctctaaag ccaagtgaat cgcgcaatac accggttcca
     1981 attttttgtg aaatagatgc cgcgctattt tctacattat ctaatgcagg aacaaataaa
     2041 tgcgccagca taacagaacc taagccttca ttcattaaaa ttttgaatgg ataaaattcc
     2101 aatgaatcta aatgtgcata ggaatgattt actaccggca tggttacgtg actatctgca
     2161 tcaacatcac cgtgacccgg aaaatgtttt ccgcaggcaa gaatattatt atcctgcaat
     2221 gcattcatat acattaaacc ctttagcgca acattatatt tatcttctcc aaaagccctg
     2281 tcgccgataa ccggattttt ataattatta ttaatatcga tatccggtgc aaagttggca
     2341 tgtacaccta aacgttttaa ttgttgtgca ataattgtcc ctacttcata aacggttgag
     2401 tcgtgcgtca ttgcacccca ggttaattgt cgctgaaacg aaataacaga atccaatcgc
     2461 attgcagggc cccattcagc atccatggca atcataagcg ggatattcga catactttga
     2521 taattgttgg tcagttttac ctgacgaacc ggaccaccct gcataaaaat gagaccacca
     2581 atttgatatt tttttatcag cgtttcaatt tcttttacat gcgctgcatc tttattggaa
     2641 tatgcagcaa ccataaataa ttgcccgatt ttttgatcga gggtcatggt tgccatgagg
     2701 gaatctaccc aagggtaatt ttggtcgtaa aacggaattt tgtgctccac gtcattttct
     2761 ggaagttggg tattgaatga tgcagaagca aaacctacag taaacagtaa tatgaagagt
     2821 gattttatat acacggttga tgttttattt tgaaatttgc cttgttttat gcaaaaataa
     2881 ccttagctgt ttaggtaaga gcacaattta cgccaaaaat gatgttatca tctcacaaat
     2941 taagacaatc agccaagcgt aatatcctta cctttgcaag taaacggcag aaacgtttta
     3001 tttgatacaa ctttaggcaa attattttag acaaccaggg agaggagtga catgtcagat
     3061 ataattcatt tattaccgga ttcgatagcc
//
LOCUS       DAORKW010000452         3090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1797_length_3090_cov_4.144646, whole genome shotgun sequence.
ACCESSION   DAORKW010000452 DAORKW010000000
VERSION     DAORKW010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1797_length_3090_cov_4.144646"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="PLB46_13960"
     CDS             complement(<1..1416)
                     /locus_tag="PLB46_13960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HRF77678.1"
                     /translation="MKRLLIILMLSYSVQSIAQQYDETFNGTGLLIGQSNYDGVNMID
                     NAKDILYDPATGNLVMWNELSYSGGTLHRYLPNGTLDPTFGVGGNVEIHMELDNRFES
                     LFATDDGYQLAGHVTPEHNVFIPAVLELNYDGSINTDFGIDGLVTEASIDSFIPFHAT
                     KGPSGKITLAGWDYDALFADPAAGMARFDDNGNFEEDFGFLTFGVNVHTEFRSALELS
                     DGRVIGYGHTYNETADSTVGYIACFKDNGDLDLSFGDNGIVIPKPDTTDIHNYQFYRA
                     IQGNDGSIYLAGILAMIPDYFLYVVKMDVNGNVITEYGKNGAVFTKATDITECKQILV
                     TPANEVYLLSIGHPLDDATVETHIFKILADGTPDYSFTNGNPGTFRINIEDAQDVIGT
                     KMVMQTDGKIVVTGSVDGVGGNRDFFVCRITSNSEPMAIQQTPMFDFKLSPNPAESLL
                     SIETDEIISFYEVISNTGQILN"
     gene            1574..2860
                     /locus_tag="PLB46_13965"
     CDS             1574..2860
                     /locus_tag="PLB46_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRF77679.1"
                     /translation="MKYLPIDSQLFIQNRNNYTKHLKPNSIAIFNSNDEMPFNADQTH
                     PFKQNSDIFYLSGIDQEESILLIYPDCPNPKYREALFLKKTSAYIAVWEGHKYTEAEA
                     TATSGITTVHWKEEFPAIFNMLMNHAENVYLNTNENDRASIIVTSRDARFVADTKSRF
                     PLHNYERSAPIMAKLRTSKSEFEKQLIQTACDITNKAFRRVLAFVKPGVMEYEIEAEI
                     MHTFLSNRATRQAYGSIIASGANACVLHYVDNNQPCNDGDLLLMDFGAEYANYCADLT
                     RTIPVNGKFTQRQKDVYNAVLRVQREAIKMMHVGMILKDFNAEVGKVMEKELLGLGLI
                     TDTDVKNQNPDNPAYKKYFMHGTGHHLGLDVHDIGDHYAPLAEGAILTCEPGIYIPEE
                     NIGIRIENDIMITSNGKYDFMKDCPVEVEEIEGLMC"
BASE COUNT          999 a          588 c          578 g          925 t
ORIGIN      
        1 atttaatatt tgaccggtat ttgaaatgac ctcataaaaa ctgattattt catctgtttc
       61 tattgacaat aatgattcag caggattggg tgaaagctta aaatcaaaca tcggtgtttg
      121 ttgaatagcc atcggttctg aatttgaagt aatccggcaa acaaaaaaat ctctattacc
      181 tcctactcca tctaccgaac cggttacaac tattttgcca tcagtttgca taaccatttt
      241 tgtaccaata acatcttgtg catcttcaat atttattcta aaagttcccg gattaccatt
      301 tgtaaaactg taatcgggag tgccatcagc aagaatttta aatatatgcg tttccacagt
      361 tgcatcatcc aatggatgac caatgctgag caaatacact tcattagcag gagttaccaa
      421 aatttgttta cattcagtta tgtcagttgc tttggtaaat acagcaccat ttttgccata
      481 ttctgttata acatttccgt tcacatccat tttaacaaca tatagaaaat aatccggtat
      541 catagcaaga atgcctgcta aataaataga gccatcatta ccctgaatgg cacgataaaa
      601 ttggtaatta tgtatatctg tagtatcagg ttttgggatt acgattccgt tatcaccaaa
      661 tgaaagatca agatcaccat tatctttaaa gcaagcaata tatccaacag ttgaatcggc
      721 tgtctcatta taggtgtgac cgtaaccgat tacacggcca tcacttaatt caagtgctga
      781 acgaaattct gtatgcacat tgacaccgaa tgttaagaac ccaaaatcct cctcaaaatt
      841 tccattatca tcaaaacgag ccatacctgc tgcagggtct gcaaacaaag catcataatc
      901 ccaacccgca agagttattt tcccggaagg acctttagtt gcatgaaaag gaataaatga
      961 atctatgcta gcttcggtta ccaatccatc gattccaaaa tcggtattta tactgccgtc
     1021 ataatttaat tccaacacag caggtataaa aacgttatgt tcaggtgtaa catggcctgc
     1081 aagttggtaa ccgtcatctg tagcaaataa actctcgaag cggttgtcta attccatatg
     1141 gatttctaca ttaccaccta caccaaaagt ggggtccagc gtaccatttg gcaaataccg
     1201 gtgtaaagta cctcctgaat aacttaactc attccacatt accaaatttc ccgttgcagg
     1261 atcatagaga atatcctttg cgttatcaat catattaaca ccatcataat tggattggcc
     1321 tattagtaac cctgttccat tgaaagtttc atcatattgt tgagcaatcg attgaacgct
     1381 ataactgagc attaaaataa ttaataacct tttcatttta ttttttttgt aaagatgcca
     1441 gaaatagcaa gtttgataaa taccacaaac aggggagtgt ttcttttaca tgatttatac
     1501 agtgaaacag aaagatagac gttgagttcg aactcatttt tcattacctt tagcacatca
     1561 attttaaaaa tatatgaagt atttacctat agacagccag ttgtttatcc aaaacagaaa
     1621 caactacaca aaacatttaa aacctaacag catagccatt ttcaacagta atgatgagat
     1681 gccgtttaat gctgaccaaa cccatccttt caaacaaaac agcgatattt tttacctgag
     1741 tggaattgac caggaggaaa gtatactgtt aatttatccc gattgtccta accctaaata
     1801 tcgcgaagcg ctatttttga aaaaaaccag cgcctatatt gctgtttggg aaggacataa
     1861 atatactgag gcagaagcta cagctaccag tggtattaca actgtgcatt ggaaagaaga
     1921 atttccggcc atattcaata tgttaatgaa ccacgccgaa aatgtgtatc tgaataccaa
     1981 tgaaaacgac agagccagta taattgtaac ttccagagat gcccgttttg ttgctgatac
     2041 aaaaagtcgt ttcccattac ataattacga acgcagcgca ccaattatgg caaaattgcg
     2101 caccagtaaa agtgaatttg aaaaacaact tattcaaact gcatgcgata ttaccaataa
     2161 agcctttcgt cgcgttttag cctttgttaa acccggtgtg atggaatatg aaattgaagc
     2221 tgaaattatg catacatttt taagcaacag agcaacccgt caggcttatg gaagtattat
     2281 tgcgagtggt gccaatgcat gtgtattaca ttatgtagat aataatcagc catgtaatga
     2341 tggtgatttg ttgttgatgg actttggtgc ggaatacgcc aattattgtg cagatttaac
     2401 acgtactatt ccggttaacg gaaaattcac gcaacgccag aaagatgtat acaacgccgt
     2461 tttacgtgtg caacgtgaag ctataaaaat gatgcacgta ggtatgatat taaaagattt
     2521 taatgctgag gtaggcaaag tcatggagaa agaattattg ggtctggggc taattaccga
     2581 taccgatgta aaaaatcaaa atcctgataa tcccgcgtac aaaaaatatt ttatgcatgg
     2641 caccggccat catttaggtt tagatgtaca cgatataggc gaccattatg caccacttgc
     2701 tgaaggcgcc attttaacct gcgaacctgg catttatatt ccggaagaaa atattggcat
     2761 tcgcatcgaa aacgatatca tgataacctc caatggaaaa tatgatttca tgaaggattg
     2821 tccggttgag gtggaagaga ttgaggggtt gatgtgctaa tgtgttgatg tgctgatgtg
     2881 ttaatgtgct gatgtgttaa tgtgctgatg tgctgattaa tgtaccaatg cgggaatgta
     2941 ccgatgtacc aatgcgcgaa gcgtccgctg aggcggatat tgatgtgttg atgtgctgat
     3001 ttgtttatgt gctgattgac agccgggttc ggaggtggaa tttcattcag tgatacctta
     3061 gtggtcaacc cacgtgttga cctgacgatt
//
LOCUS       DAORKW010000453         3079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1812_length_3079_cov_4.681878, whole genome shotgun sequence.
ACCESSION   DAORKW010000453 DAORKW010000000
VERSION     DAORKW010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1812_length_3079_cov_4.681878"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PLB46_13970"
     CDS             complement(<1..1113)
                     /locus_tag="PLB46_13970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRF77680.1"
                     /translation="MQKAYEKIQLQVEGMTCANCALGITRLLEQKGMDDVYVNFATGE
                     VHFANAGDKQLPELVNGIESLGYKVVNEQLAATQKKSAFTTTEKKLLFTIPFTLLLLL
                     AMVPGLSFLHQPLIQLLLCLPVYIIGFFHFGKSALGSIKSGIMNMDVLIFTGSTAAFG
                     YSVFGFVNQLGMDYQFFETAATIITLVLLGNVIEHRSVKQTTTAIAALKQLQPLTAKR
                     VHFDLLSGGDHIDEIPATQLVKNDVILINTGDSIPADGIVIFGTAYVDESMLSGESAP
                     VYKQKNDIVSGGTINTTGILKCKITAPPGQTTLDAIIELVKQAQTQKPPVQRLADKIS
                     AIFVPVVLTLALLTFLISYFGFHVVLQDALLRSIAVL"
     gene            1233..2003
                     /locus_tag="PLB46_13975"
     CDS             1233..2003
                     /locus_tag="PLB46_13975"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRF77681.1"
                     /translation="MNNSNTNSRRSFLKKGALAAIGVGVLNSLTANELYAGTVNGNLE
                     MPPVDGAFELPKLDYAYNALEPFIDAKTMEIHHSKHHQTYVTKLNEAIEKAPELKGKS
                     LMELVANINAAPESVRTAIRNHGGGHFNHSFFWKSMSPDAKNTAASAELTAAINAKWT
                     SMDNFKAEFAKSATGVFGSGWAWLIKDKENNLSLTTTANQDSPIMDIAAMRGKPILGI
                     DVWEHAYYLKHQNKRADYIADFWNVIDWNQVSKWYAEL"
     gene            2119..2193
                     /locus_tag="PLB46_13980"
     tRNA            2119..2193
                     /locus_tag="PLB46_13980"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2154..2156,aa:His,seq:gtg)
     gene            complement(2224..>3079)
                     /locus_tag="PLB46_13985"
     CDS             complement(2224..>3079)
                     /locus_tag="PLB46_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRF77682.1"
                     /translation="VIRNPKSKEPIYPLAVIEDGENARLKLIEGKRNLELAAEHISSA
                     DTNVQILTRVDVNVAAGVSSAIKELGITEVVMGWHEKVSTSDRIFGSVLHKIVQTSDQ
                     MLWVSKIISPLNNFNRIAILMPPNAEFESGFFRWLEGVKHLSHHIGAKCIFWGERKTL
                     ELIDAINDKNLKLNAEYSEFTDWDDIKSIAGNLKQGDLFIVVSARQKSVSYFSSLEQV
                     PLRLNKHFEAHSFILLYPFQMDSGSNDMNFQYSVLSPSPFKENFERINTIGKTVYKAF
                     SSSNKKGK"
BASE COUNT          936 a          624 c          577 g          942 t
ORIGIN      
        1 cagtactgca atacttcgca acaatgcatc ttgtaaaaca acatggaacc cgaaataact
       61 aattaaaaaa gtaagtaatg caagtgttaa cacgaccgga acaaaaatgg cgctgatttt
      121 atctgccagt cgttgcacag gtggtttttg tgtttgcgcc tgttttacca attctataat
      181 agcatctaaa gtagtttgtc ccggcggcgc ggttattttg cattttaaaa ttccagttgt
      241 attaattgtt ccacctgaaa caatatcatt tttttgtttg taaacaggtg cactttcacc
      301 acttaacatc gactcatcta cataggcagt tccaaatatc acaattccat cagccggaat
      361 gctatcgccg gtattaataa ggataacatc atttttaacc aattgtgttg ccggaatttc
      421 gtcgatatga tcgccgcctg ataataaatc gaaatgcacc cgtttggcgg tgagcggttg
      481 taattgtttg agcgcagcaa ttgctgtggt ggtttgttta acacttcggt gttcaattac
      541 attgcccaat aacaccaagg tgataatagt agcagctgtt tcaaaaaact ggtaatccat
      601 gcccagctga ttaacaaaac caaacacact atatccaaat gcagcggtac tgccggtaaa
      661 tatgagtacg tccatattca taataccgga cttaatagaa cctaaggcac tttttccaaa
      721 atgaaaaaat cctattatat aaacaggtaa acacaataat aattgtataa gcggctgatg
      781 taaaaacgaa agtccgggca ccattgccaa taataaaagc aatgtaaatg gaatggtgaa
      841 aagcaatttt ttttcagtag tggtaaaggc cgattttttt tgtgtcgctg ctaattgttc
      901 gttaaccact ttataaccca atgattcaat cccatttacc aattccggca attgtttatc
      961 acctgcgttg gcaaaatgca cctctccggt agcaaaattg acgtacacat catccattcc
     1021 cttttgttcc aacagacggg taatgccaag tgcgcaattt gcacaagtca tgccttctac
     1081 ctgcaattgt attttctcgt acgctttttg catatatccg aacgtaaagg taattattct
     1141 gttaacgata gggatagcgt taatcacatt cccataacta ctattccacg aaaaacaata
     1201 ccttcatgta ataaattgaa caaatactat ttatgaataa ttcaaataca aattctcgtc
     1261 gcagctttct taagaaggga gcccttgctg caattggtgt tggcgtactt aattctttaa
     1321 ctgccaatga actttatgcc ggaacggtaa acggtaatct ggaaatgcct cctgtggatg
     1381 gcgcattcga attgccgaaa cttgactacg cctataatgc tttagaacca tttattgatg
     1441 ctaaaaccat ggaaattcac cacagtaagc atcatcaaac ctatgtaacc aaactcaatg
     1501 aggcaattga aaaagcgcct gaactgaaag gtaagtcgct gatggagttg gttgccaata
     1561 ttaatgcagc acctgaatct gttcgtactg ccatccgcaa tcacggtgga gggcatttta
     1621 atcactcatt tttctggaaa tcaatgtcgc cggacgctaa aaatacggct gcttctgccg
     1681 aattaactgc tgctatcaac gccaaatgga ccagcatgga taatttcaag gcggaatttg
     1741 ccaagtcggc aacaggcgtt tttggttcag gatgggcttg gttaatcaag gataaggaaa
     1801 ataacctgag cttaaccacc actgctaatc aggacagtcc cattatggat attgctgcca
     1861 tgcgcggtaa accgatttta ggtatagatg tatgggaaca tgcctattac ctgaagcatc
     1921 aaaataaaag agccgattat atcgccgact tctggaatgt aatagactgg aaccaggtga
     1981 gtaaatggta tgccgaatta taaacaggca ttgtagtctt catttgccga tagtaaagca
     2041 caagattttt tgctatcggc aaatttcatt ttaaagtgat aattttttgc gttaactttg
     2101 caccactttt tataaatggt gactgtagct cagtttggtt agagcatcgg attgtggttc
     2161 cgagggtcgt gggttcgagc cccatcagtc acccatcaaa gatccgccct tcagcggatt
     2221 tttttatttt ccctttttat tgctcgatga gaatgcttta tataccgttt ttccaatggt
     2281 attgattcgt tcaaagtttt ctttaaacgg cgatggcgat aatacgctgt attggaaatt
     2341 catatcgtta gaaccggaat ccatttggaa tggatataac aaaataaaac tgtgtgcttc
     2401 aaaatgttta tttaatcgca acggtacttg ttcaagtgaa gagaaataag aaacactttt
     2461 ttggcgtgca ctaactacaa taaataaatc accttgtttt aaattaccgg caatactttt
     2521 aatgtcatcc caatcggtaa actcgctgta ttccgcattg agttttaaat ttttatcgtt
     2581 gatggcatct atcaactcca atgtttttct ttcaccccaa aaaatacatt ttgcaccaat
     2641 atgatgtgac aaatgtttta cgccttctaa ccaccggaaa aaaccacttt caaattcggc
     2701 attaggaggc attaaaatgg caatacgatt aaaattattg aggggagaaa taattttact
     2761 cacccacaac atttgatcgg atgtttgaac aattttgtgt aacaccgagc caaatatgcg
     2821 atcgctggtg ctcacttttt cgtgccagcc catcactacc tctgtaatac ctaactcttt
     2881 aatagcactg gatacacctg cagccacgtt tacgtcgacg cgggttaaaa tttgtacatt
     2941 ggtatcggcg ctggaaatat gttctgcagc aagttctaaa ttgcgtttac cttcaattaa
     3001 ttttaatcgg gcattttccc catcttcaat aacggcaagc ggataaatag gttctttaga
     3061 ttttggattg cgaattact
//
LOCUS       DAORKW010000454         3074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1816_length_3074_cov_5.013249, whole genome shotgun sequence.
ACCESSION   DAORKW010000454 DAORKW010000000
VERSION     DAORKW010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1816_length_3074_cov_5.013249"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(667..1122)
                     /locus_tag="PLB46_13990"
     CDS             complement(667..1122)
                     /locus_tag="PLB46_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77683.1"
                     /translation="MQFIKSTSITLLLLAMLTSCANKSDKHSHDESATTQHHEEESKA
                     LYLNNGEKWLVNEEMKPYILDAESILNSYVANGGNDYKQLATQLKEKNAALIKSCTMS
                     GESHEVLHIWLHPHLALTDSLMQAKSIEEANTTITALQNSLTSYHAYFQ"
     gene            complement(1297..2211)
                     /gene="dapA"
                     /locus_tag="PLB46_13995"
     CDS             complement(1297..2211)
                     /gene="dapA"
                     /locus_tag="PLB46_13995"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRF77684.1"
                     /translation="MHNYSVSVTHKFRGTGVALVTPFNSDGSIDFTGLNKLINHVIDG
                     GVEYLVAMGTTGESATLSEQERFEIIDFTLAASNGRVPVVGGFGGNNTMEIIHSIQRY
                     KSHCSGKKYIDAILIASPYYNKPSQEGIYQHYMAIDQVVEVPIILYNVPGRTSSNMEA
                     STTVRLANDAKHVIAIKEASGNFVQCMRIIRDAPAGFLVISGDDPITLPFIAMGMQGV
                     ISVVGNAYPKDFSNMVRESLQHQFSTANPLHYKLLDFTELIFKEGSPAGVKYALKKLG
                     ICGDKVRLPLWKISESLEKQLDDCMGRM"
     gene            complement(2302..2823)
                     /locus_tag="PLB46_14000"
     CDS             complement(2302..2823)
                     /locus_tag="PLB46_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77685.1"
                     /translation="MIKSIKNILFGRFIRSKPYTDTYERKTKEYAKIKTMAFLVDGSD
                     PIALRELINYLHKYIDEGKKISFIGYVKKLPPFEDEDIIWVTNKDMNWIGIPKMQKIK
                     HFIEKEFDVLINTSMQVNRPLEYISTYSKAALRIGKFDPKKTYCYDFMMRIEHDNIYA
                     YLEQVERYLKMVK"
     gene            complement(2833..>3074)
                     /locus_tag="PLB46_14005"
     CDS             complement(2833..>3074)
                     /locus_tag="PLB46_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77686.1"
                     /translation="QMFFEVFFEDFCHFFGIVKEPLGGAHSDPEEMAKILKKHLKKHL
                     KELAGIDPEVRIQQRIEKFDEMGVFEELNAGNAKA"
BASE COUNT          963 a          568 c          504 g         1039 t
ORIGIN      
        1 ccctcctcat tgcgatgtat ttcgcattca gattattttg gtccgcctcg gcggcctttt
       61 tttatctggg taacaatgtg gtttatgcaa ttttttaaac tccaaccaac aacatgtcta
      121 tttaaataaa atgggttgca attacgaatc actttttagg ttatttaggc gcgatagtat
      181 ctcgtttatt aaataaatat tgctgacact ttaattgaat gcatcataat atatggacga
      241 taggatagga ggacatttta aaatttcgat caaacataga agtaggaata gaagttcaaa
      301 ataaaattca actcacatgg taaaatagtg ttcaacgtta tgcggctaat ctacctcgtc
      361 tctggttctg aatttggaaa tataatgttt gggttttata gataaccaga ttttagttgc
      421 ccatattatt ccaaaaactc caccgagcgt aacaattatc aatcctagtt ttacggtttt
      481 agaacaagga tgcaacaaaa taggaataag cgcaatcccg aaaccaattg caaatgggaa
      541 aatggcaact ttaagccaat caacaatgtt taatagtaat gtgagtattc gatacaccat
      601 acttttacca caacagatta atcggtttaa ttcaataata taatattttc gtggtttttc
      661 acctcatcat tgaaaatacg catgatacga agttaatgaa ttttgtaaag cagttattgt
      721 tgtatttgct tcttcaatac tttttgcctg cataagcgaa tcggttaatg caagatgtgg
      781 atgcaaccaa atatgcaaaa cctcatgact ttcgcccgac atggtacaac ttttaattag
      841 tgctgcgttt ttttctttta gctgtgtagc aagttgttta taatcattac caccattggc
      901 aacataacta tttaaaatac tttcagcatc taaaatatag ggcttcattt cttcattcac
      961 caaccacttt tctccattat tgagataaag ggctttactt tcctcttcgt gatgttgtgt
     1021 ggttgcagat tcgtcgtgtg aatgtttatc cgatttattt gcacaacttg tcagcattgc
     1081 caacaatagg agtgtgatac ttgtcgattt tataaattgc atagtgattt tattatggat
     1141 taatattaaa gctaaataat aacaaatcag gtattaatag ttgatttgct gtatgattat
     1201 tataccactc aaccaagttg acttaaatta taaatgaaat gccaatccaa caaaaaagga
     1261 aaaataattt gctgcctttt taagggcaaa tttcaattac atcctcccca tacaatcatc
     1321 caattgtttt tctaaacttt cgcttatttt ccataaaggc aaacgcactt tatcgccaca
     1381 aattcccagt tttttaagtg catattttac acctgcggga ctaccttctt tgaaaatgag
     1441 ttctgtaaaa tcaagtagtt tataatgtaa cggattagct gtactaaatt gatgttgcaa
     1501 actttcgcgt accatattgg aaaagtcttt cgggtaagca tttcctacca ccgaaataac
     1561 accctgcatg cccattgcaa taaatggtaa agtgattggg tcatctccac ttataactaa
     1621 aaaacctgcc ggcgcatcgc gaataatgcg catacattga acaaaattgc cacttgcttc
     1681 tttaatggca attacatgtt tcgcgtcatt agccaaacga acagtagtag aagcctccat
     1741 attggagctt gttctccccg gaacattata taaaataatt ggcacctcaa ccacttggtc
     1801 aatggccatg taatgctgat aaattccttc ctgtgaaggt ttattataat aaggtgaagc
     1861 aattaaaatg gcatcgatat actttttgcc actgcaatgt gatttataac gttgaatgct
     1921 atggataatt tccatggtat tgttaccacc aaaaccgcca acaacaggca ctctgccatt
     1981 tgaagctgct aaggtgaaat cgataatttc aaaacgttcc tgttccgaaa gtgttgcact
     2041 ttcaccggta gtgcccatgg ctaccagata ttccacacca ccatcaataa cgtggttgat
     2101 gagtttgttt aaaccggtaa aatctatact gccatcacta ttaaatgggg taactaatgc
     2161 aacaccggtt cctctaaatt tatgagttac ggaaacagaa taattatgca taaatgaaat
     2221 ttgttgttag cgaaaaataa aatacaaagt tgagtaaaaa agtggaggaa ttatatttgt
     2281 tgcgtataaa caaattcggg tttatttcac catttttaaa taacgttcca cctgttcgag
     2341 ataggcgtaa atattatcat gttcgatgcg catcataaag tcgtaacaat acgttttttt
     2401 cgggtcgaat ttacctatac gaagtgcagc tttgctgtag gttgaaatgt attccagtgg
     2461 ccgatttacc tgcatcgagg tattaatcag cacatcaaat tccttttcaa taaaatgttt
     2521 aattttttgc atcttcggaa taccaatcca gttcatgtct ttgttggtta cccaaataat
     2581 atcttcatct tcaaacggag gtagtttttt aacatatccg ataaagctga tttttttccc
     2641 ttcatcgata tatttatgta aataattaat gagttcccgt aatgcaattg ggtcagaacc
     2701 atcaacaaga aatgccattg tttttatctt ggcatattcc tttgtttttc gctcataggt
     2761 atctgtataa ggttttgaac gaataaacct tccgaataaa atatttttga tactttttat
     2821 caatggctat agttaggctt ttgcattacc tgcatttaat tcttcaaata cacccatttc
     2881 atcaaacttt tcgatgcgtt gctgaatacg cacttcaggg tcaattcctg ccagttcttt
     2941 caaatgtttt ttgaggtgtt ttttgaggat ttttgccatt tcttcaggat cgctgtgtgc
     3001 accacccaat ggttctttaa cgataccgaa gaaatggcaa aaatcctcaa aaaacacctc
     3061 aaaaaacatt tgaa
//
LOCUS       DAORKW010000455         3042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1838_length_3042_cov_4.668564, whole genome shotgun sequence.
ACCESSION   DAORKW010000455 DAORKW010000000
VERSION     DAORKW010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1838_length_3042_cov_4.668564"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1344
                     /gene="murF"
                     /locus_tag="PLB46_14010"
     CDS             61..1344
                     /gene="murF"
                     /locus_tag="PLB46_14010"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRF77687.1"
                     /translation="MMLTTADELYQLYLKSSGVTIDNRKITGGEIFFGLPGEKVNGGA
                     FAQAAFDNGASYCVVDDAKYAINDNCIVVEDVLLTLQQLAAQHRKTFNFPVLALTGSN
                     GKTTTKELIATVLSKKYNVAATKGNLNNHIGVPLTILSIKNDCNFAVIEMGANHQHEI
                     EGYCTYADPDFALITNIGKAHLEGFGGEAGVLKGKSELFNYVREKNGVLFIHSSERRL
                     IENFGTYNKTVRYGFDDNDYTTGKLVSGNEFATIVLDEEVTIHSNLVGDYNANNIIAA
                     ACIGKYFDIGFQEIKDAIEQYFPDNNRSELKEIGGNYFILDAYNANPSSMKAALENFS
                     KLKASHKVAIIGDMLELGEYAAQEHQSIIELVLQSEIQQIVTVGPLFFQHQKNNIVAF
                     QHAADAKIWLENQRLQGSTILLKGSRGMKLETIVQ"
     gene            complement(1370..2530)
                     /locus_tag="PLB46_14015"
     CDS             complement(1370..2530)
                     /locus_tag="PLB46_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HRF77688.1"
                     /translation="MINRLLTPVRIKEFLNDWTLQSYHWGKKIGINTGELPDTNGCLI
                     AIIGVNEGRGSLQNSGSATGADTIRQHLYRLAVNDYPLQVIDAGDVLIGETIEETYAN
                     LKMVMEWFLGQQILPIIIGGSQDITIGQYAGYEFLGQAVNLMVIDERIDLDDQEYLPL
                     ARTWINNIFNYDPKYLFNYAGIAYQTHFNSPEILKAIDALYFDAIRLGRLKENIQFAE
                     PEVRDADLLSIDIASIRGADAPGHAHATPNGLSGEEMCQIMRYAGMSDKITSVGIYEY
                     NPMFDNQELTAQLIAQMIWYFVDGYYNRKSDYPIVSEKDFTKYIVNIADNEYDLTFWK
                     SIKTGRWWVEVPFIVQQRFERHQLVPCAYEDYEAAVKNEVPDKWLRIFHKLG"
     gene            2711..>3042
                     /locus_tag="PLB46_14020"
     CDS             2711..>3042
                     /locus_tag="PLB46_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77689.1"
                     /translation="MQDFFNSEFLHNTIRQWCWLAGVIVFVIIFKRYISKLISLLLFK
                     IVQKTEAGELTEEFLKLILKPLQYFIVLQTIYLGFKTLNYPFIETDEVVKGYVITFLY
                     GTYRFLLI"
BASE COUNT          958 a          530 c          551 g         1003 t
ORIGIN      
        1 cgggggttta attttttata aaagccccca cctttggtgg gggttgttta cttttactcc
       61 atgatgttaa ctactgcgga tgaattgtac caactttatt taaaatcaag tggagttact
      121 atcgataacc gaaagattac cggcggtgaa atatttttcg gacttccggg cgaaaaggta
      181 aatggtggtg catttgcaca agctgcattc gataatggtg caagctattg cgttgtggat
      241 gatgcaaagt atgccattaa tgataattgt attgttgtgg aggatgtact gcttacttta
      301 caacaacttg ccgcacaaca tagaaaaaca tttaattttc ctgtattagc cttaaccggt
      361 tccaatggta aaacaactac caaagaatta attgcaactg tgctgagtaa aaaatataat
      421 gttgcagcaa caaaaggcaa tttaaataat catatcggtg ttccgcttac cattttatcg
      481 attaaaaatg attgcaattt tgctgtaatc gaaatgggag caaatcatca gcacgaaatt
      541 gaaggttatt gcacctatgc cgaccctgat tttgctttga taacaaatat cggtaaagcg
      601 cacctcgaag gttttggtgg tgaagccggt gtgctgaaag ggaaatcgga attgtttaat
      661 tacgttcgtg aaaaaaatgg cgtattattt attcatagca gtgaacgtcg tttaattgaa
      721 aattttggca catataataa aactgtgcga tatggttttg atgataatga ttatactact
      781 ggcaaattag tttcgggcaa tgaatttgca acaattgttt tagatgaaga agtaaccatt
      841 cactcgaatt tagttggcga ttataatgcc aataatatta tagcagctgc atgtatcggt
      901 aaatattttg atattggttt tcaggaaata aaagatgcta tcgaacaata ttttcccgac
      961 aataatagaa gtgaactcaa agagattggt ggtaactatt ttattttaga tgcgtacaat
     1021 gccaacccgt caagtatgaa agcggcactt gaaaattttt caaaattaaa agcatcacat
     1081 aaggtggcca tcattggtga tatgctggaa ttaggggaat atgctgctca agaacaccaa
     1141 tccattattg aattagtttt acaatctgaa atacaacaaa tagtcacagt aggaccttta
     1201 ttttttcagc atcaaaaaaa taatattgtt gcatttcaac atgctgcaga tgcaaaaatt
     1261 tggcttgaaa atcagcggtt acaaggtagc acgatattat taaaaggctc cagaggaatg
     1321 aaactggaaa caattgttca gtaagtggag taaattaatt taataatact tatcccaatt
     1381 tgtggaaaat acggagccat ttatccggca cttcattttt tacagcagcc tcataatctt
     1441 catatgcaca gggaactaat tggtggcgct caaaacgttg ttgaacaata aaaggaactt
     1501 ctacccacca acgacctgtt tttatacttt tccaaaatgt gaggtcatat tcattatccg
     1561 caatgttaac gatgtatttt gtaaaatctt tttctgacac aatcggataa tcacttttgc
     1621 gattgtaata cccatccaca aaataccata tcatttgtgc aatcaattgt gctgtcaatt
     1681 cctgattgtc gaacattgga ttgtattcat atatgccgac agaagtaatt ttatcgctca
     1741 taccggcata acgcataatt tgacacattt cttcgccgga taaaccattt ggcgttgcat
     1801 gtgcatgtcc cggtgcatca gctccgcgaa tggaagcaat atcaatactc aataaatcag
     1861 catcgcgcac ttcgggttct gcaaactgga tattctcttt taatcgacct aatcggatag
     1921 catcaaaata taaggcatca attgccttta atatttcagg ggaattaaaa tgtgtttggt
     1981 aggcaatacc tgcataatta aataaatact ttgggtcgta attaaaaata ttatttatcc
     2041 aggttctggc aagtggtaaa tattcctggt cgtctaaatc gatacgttca tcaattacca
     2101 ttaaattaac tgcttgtcct aaaaactcat atcctgcata ttggccaata gtaatatcct
     2161 gactgccacc aataattata ggtaatattt gttgacctaa aaaccactcc ataaccattt
     2221 ttaagttggc ataggtttct tctatcgttt ctcctattaa cacatcaccg gcatcaatta
     2281 cctgcaaagg gtagtcattt acggcaagcc gatacaaatg ttgtctgata gtatctgctc
     2341 ctgtagcaga gccactgttt tgtaagctgc cacgaccttc atttactccg attatggcga
     2401 ttaaacatcc atttgtatcc ggcaattcgc ctgtgttgat accaattttt ttaccccaat
     2461 gataactttg gagtgtccag tcatttaaaa actctttaat tcgaacaggt gtaaggagtc
     2521 tgttaatcat ggtataaaat tgaggtttaa tatggtacca atgtaaaaca gtttacacct
     2581 atgtggtttt agtttgtcta tttattcagt catttaaagc tttcaaaact ggctaccgta
     2641 tgcactttgg tcaatagtgc catgacctgc atttattttt ctttaaattt gtagcataat
     2701 ttcaggtagc atgcaggatt tttttaactc agaatttctc cacaatacca ttcgacaatg
     2761 gtgctggctt gccggtgtta ttgtttttgt gataattttt aaacgatata tatccaaact
     2821 gataagtctt ttattgttta aaattgttca gaaaacagag gcaggtgaac ttactgaaga
     2881 gtttctgaag ttaatcctga aaccacttca atattttata gttcttcaaa caatttattt
     2941 gggatttaaa acacttaatt atccttttat cgaaactgat gaggtggtaa aagggtatgt
     3001 tataactttc ctgtatggca cctaccggtt tttactcata tt
//
LOCUS       DAORKW010000456         3040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1840_length_3040_cov_5.806030, whole genome shotgun sequence.
ACCESSION   DAORKW010000456 DAORKW010000000
VERSION     DAORKW010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1840_length_3040_cov_5.806030"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="PLB46_14025"
     CDS             <1..1148
                     /locus_tag="PLB46_14025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77690.1"
                     /translation="YLNDGGEINTGHVRIYNWNGSAWIQKGADIDGEAADDRSGWSVS
                     MPDANTVAIGAYQNGGNGNDAGHVRIYEWNEVAWIQKGGDIDGEVAEDLSGHAVSMPD
                     ANTVAIGAHRNDGGSYHAGHVRIYFWDGLNWVQKGADIDGEAADDYSGSAVNMPDANT
                     VAIGAWGNDGNALNAGHVRVYTWDGVTWIQKGTDIDGAAANDEFGYSISMPDANTIAI
                     GAHRADGTGLDAGQAHIYKWDGSAWMLLSSIDGEMVGDESGCSVSMPNANTIAIGARY
                     NSNSGYHAGHTRIYSSADEVGVLETNFSNQIVVYPNPTKGELYIDLGESNYNPTITIT
                     NIWGQQILKTKYNDINYLQLTIPGPAGMYFIEVSSGDKKTILKIIKK"
     gene            complement(1246..1899)
                     /locus_tag="PLB46_14030"
     CDS             complement(1246..1899)
                     /locus_tag="PLB46_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77691.1"
                     /translation="MKQVILIITYLFCVQLQAQLVAPDRQETPALGTGIRYENTIYWD
                     IHYVQHFKCDTTHEAPYYMQLSLSTPMYLINTGNKNLHVQIGRQWDKNNWFVNGLVGV
                     NIFGYEDVMANGMGLSADLLLQGGIHKNVWFAGAEIGYKNTIFTTFNFENPVNDLSST
                     TQFNTNGNYIIGAFGGFRFLHQFSAQAHLDYQINRDFTSYPPYTVPYSGGLTLLYHF"
     gene            complement(1906..2886)
                     /locus_tag="PLB46_14035"
     CDS             complement(1906..2886)
                     /locus_tag="PLB46_14035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRF77692.1"
                     /translation="MINKNLILAACCVALMTACVQQDDFNELVRPTVTEDNSLPRFTL
                     SDGTILHLETFGDPDSTMLLVLHGGPGGDYEGLSVLQTLSNHYFVVLFDQRGSGLSQR
                     LPIEELTPPKFIEDIDEIVNTYSNNKPIYLLGHSWGGALATYYVQQHPEKVAKLLLAE
                     PGACYGAAAEVANTTAFIFSSDGLHEMLNSGSYLSFDNDNIADYRMSVFVNSDVGDYR
                     DFSSPEELQELSFTRFGFFAGYAVNDWQGNFDGTYDYDFTSGIKENYFGETLILASDK
                     SQRLGYDFQLQYHVPKFNNCAITKIENEGHFFIELNPEMALPLIDAFFLN"
BASE COUNT          972 a          539 c          627 g          902 t
ORIGIN      
        1 cttatttaaa tgatggtggt gaaattaata ccggccatgt aagaatttac aattggaatg
       61 ggagtgcctg gatacaaaaa ggtgcagata ttgatggaga agcagccgat gaccgctccg
      121 gttggtcagt aagtatgccc gatgcaaata cggtggcaat tggtgcttat caaaatggtg
      181 gtaatggaaa tgatgccgga catgtaagaa tttacgaatg gaatgaggtt gcatggatac
      241 aaaaaggagg cgacattgat ggtgaggtag cagaggatct ttccggccac gcagtaagta
      301 tgcctgatgc caacacagtt gcaattggtg cacatcgaaa tgatggagga agttaccatg
      361 ccggacatgt gcgcatttat ttttgggacg gattaaattg ggtgcaaaaa ggtgctgata
      421 ttgatggcga ggcggcagac gattattccg gttcagcagt aaatatgcct gatgcaaata
      481 cagttgccat aggagcttgg ggtaatgacg gaaatgcctt aaatgccggt catgttcgcg
      541 tttacacctg ggatggcgta acatggatac aaaagggaac agatattgat ggagcagctg
      601 caaatgacga atttggttat tcaatcagca tgccggatgc caataccata gcaataggcg
      661 ctcatcgtgc agatggaacc ggattagatg ccggccaagc gcacatctat aagtgggatg
      721 gcagcgcatg gatgctccta agtagtattg acggtgaaat ggttggtgat gagtccggct
      781 gctcggtaag catgccaaac gcaaatacaa ttgctattgg cgctcgttat aattcaaatt
      841 caggatatca tgcaggccat acgcgaattt attcttctgc tgatgaggta ggtgttttag
      901 aaacaaactt ttctaatcaa attgtagttt atccaaaccc aactaaaggt gaattgtata
      961 tcgacttagg tgaaagtaat tataatccga ctataactat aaccaatata tgggggcaac
     1021 aaatattaaa aacaaaatat aatgatatta attacttgca actcaccata ccagggcctg
     1081 caggtatgta ttttattgaa gttagttctg gcgataaaaa aacaatttta aaaattatta
     1141 aaaagtagat ttacttaaaa ttaaatagac tactatttat aataatagtg cgcaagttga
     1201 tttatgaagg catcacatta tttgttataa gtgtgttgtg ctatttcaaa agtgatataa
     1261 taaagtcaat cccccgctat atggaaccgt ataaggtgga tacgatgtga aatcgcgatt
     1321 aatttgataa tcaagatgtg cttgtgcact aaattgatgt aaaaaacgga agccgccgaa
     1381 agcaccaatt atataattgc cgttggtgtt aaattgagta gttgaactca aatcatttac
     1441 cggattttca aaattaaatg tggtgaaaat agtattttta tatccaattt ctgcaccggc
     1501 aaaccaaacg tttttatgta ttccgccctg caacaatagg tctgctgaaa gtcccatgcc
     1561 gttggccatt acatcttcat aaccaaatat gtttacccct acaaggccgt tcacaaacca
     1621 attgttttta tcccattgac gaccaatttg gacatgcaga tttttattgc ctgtgtttat
     1681 taaatacatt ggcgtgctta gcgataattg catataataa ggcgcttcat gtgtcgtatc
     1741 gcatttgaaa tgttgcacat aatgaatatc ccaataaatg gtgttttcat atcgaattcc
     1801 tgttcctaaa gctggggttt cctgtctgtc gggcgcaact aattgagcct gtaattgaac
     1861 acagaataaa tatgttataa ttaaaataac ttgtttcatt ttcaattagt ttaaaaagaa
     1921 tgcatcaatt aaaggtaaag ccatttcggg atttaattcg ataaaaaagt gtccttcatt
     1981 ttcaattttt gtgatagcac aattattgaa tttgggcaca tgatattgca gttggaaatc
     2041 gtaacccaat cgctgacttt tatctgatgc caaaatcaat gtttcgccaa aataattttc
     2101 ttttatccct gaagtaaaat cataatcata agtgccatca aagtttccct gccaatcgtt
     2161 tacagcatat cctgcaaaaa agccgaaacg cgtaaacgac aattcctgca attcttccgg
     2221 agatgaaaaa tcgcggtaat caccaacatc tgaatttaca aaaacagaca tacggtaatc
     2281 ggcaatatta tcattatcga acgataaata actgcctgaa tttagcatct catgtaatcc
     2341 atcagaggaa aaaataaatg ctgtggtatt ggctacttct gcagctgcgc cataacatgc
     2401 tcccggttct gctaataata atttggcaac tttttcggga tgttgttgca cataatacgt
     2461 tgctaatgcg cctccccatg aatgacctaa taaataaata ggtttgttgt tcgaataggt
     2521 gttaactatt tcatcgatgt cctcaataaa ttttggaggt gtgagctcct caataggcaa
     2581 tcgttgcgat aacccactgc cgcgctgatc aaatagcaca acaaaatagt gattactcaa
     2641 cgtttgtaaa acagatagtc cttcataatc gccaccgggt ccgccgtgta ataccaataa
     2701 catggtacta tccgggtcgc caaaagtttc gagatgtaaa atggttccat ctgaaagtgt
     2761 aaaacgtggt aggctgttat cttctgtaac tgtcggtctt accaattcat taaaatcatc
     2821 ctgctgcaca caagcggtca tcaaagcaac gcaacacgca gctaaaatca ggtttttgtt
     2881 aatcataaat ttttgtaaat aaataaaacg caaaagaact gttttttatg atattaattg
     2941 gtgacttaag tcccactgca tgagattgtg caattatctt aaaatgtagg atgttagttt
     3001 ctcgcaaagt cgctaagttg aagggtttgt attgtgtgtg
//
LOCUS       DAORKW010000457         3035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1848_length_3035_cov_3.382886, whole genome shotgun sequence.
ACCESSION   DAORKW010000457 DAORKW010000000
VERSION     DAORKW010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1848_length_3035_cov_3.382886"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLB46_14040"
     CDS             <1..274
                     /locus_tag="PLB46_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77693.1"
                     /translation="LVSFFIQQHSQYTTDKQFLKKFQVEEANENKTVKSFPPAFTQNL
                     MPFDELHLEFNGHHCFFLTSLKGLSAEECNRFIQLSGVLLNHLVKI"
     gene            343..882
                     /locus_tag="PLB46_14045"
     CDS             343..882
                     /locus_tag="PLB46_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77694.1"
                     /translation="MKKSAILPLICCLWLVYAGCKKETFPPEGNLIENYSFEENGVFS
                     LNGWDTSNAQYSTIVPENGGDFSVKLFPGAPPDEGYVDYIINGLVGEKTITMSCQANA
                     FGGWEGSATLKLIATGETSADLATATWDQNTWGAIDLSATATFVEGDVLMLHLSAGFS
                     PVVEPGKFVLFDLVTLSIN"
     gene            900..1349
                     /locus_tag="PLB46_14050"
     CDS             900..1349
                     /locus_tag="PLB46_14050"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HRF77695.1"
                     /translation="MASIFNAEDTAAIISRIEKLTPITQHIWGTMTVSQMLAHIQEPV
                     KVALGHTAPKRSVIGWLFGRAAKKGIINEKPFKQGLPTDSSFVIDNERNFDTEKQEAI
                     ELLRALQKAGPNGITKNKHPFFGKLSAEEWDTLTIKHLDHHLRQFGV"
     gene            1360..1905
                     /locus_tag="PLB46_14055"
     CDS             1360..1905
                     /locus_tag="PLB46_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77696.1"
                     /translation="MTQKSYPVTNYRLILLIIGITGLLMRGFLGETDVVTIILFIIQL
                     VALDLILKESNIFSTVYYRFIQICISIYIIGALFIIMHWEGGSLLLTAAVSGVVVTYI
                     IRTINKSPINILDIVKCAWVISVCFTTIAKLMHLRYADIGNYINLGLFGVMLLLFFLS
                     PVKPAPKHNNSHNELPLDQVE"
     gene            complement(1908..2864)
                     /locus_tag="PLB46_14060"
     CDS             complement(1908..2864)
                     /locus_tag="PLB46_14060"
                     /inference="COORDINATES: protein motif:HMM:NF036967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HRF77697.1"
                     /translation="MALKEKLNRNINPNTDKADELGYTNKYEQGNKRILSHDGNFNVR
                     RIGERKLVFHELLTMSWLKFALFIVAFYSIVNLIFAMMYLTVDYNGIGMTSDYEVHNR
                     FFVALFFSAQTLTTVGYGSLYPLSSTVSFVAASEALVGLMGFAIFTGLMYGRFSKTPH
                     SIRFSTNALISPYKEGFGLMFRAANERNHNLTELSVSVTLAMVINENGKPTRKFFPLT
                     IDNNKIVYFPLNWTMVHPIDDKSPLYGLTKQDVEIGEAEVLILVKGYNETSGQDIYAR
                     NSYTHQEIIWDAKFDLPYYFNEDGITVFELDKIDNYHMIEKV"
BASE COUNT          968 a          528 c          546 g          993 t
ORIGIN      
        1 acttgtatcg ttttttatcc aacaacatag tcaatacact actgacaaac agtttttaaa
       61 aaaattccag gttgaagaag caaatgaaaa caaaactgta aaaagttttc cgccggcttt
      121 tacccaaaat ttaatgccct ttgatgaatt acatttagaa tttaacggtc atcattgttt
      181 ttttctaacc agtttaaaag gattatcagc agaagagtgc aaccggttta tccaattgag
      241 tggcgtattg ttaaatcatc tggtaaaaat ataatcgttt tactttgtcg taacaatcaa
      301 ccaccgtaaa tcgttatatt tgctataaac accctggtag ttatgaaaaa aagtgcaatt
      361 ctgcctctaa tttgttgttt gtggctggta tatgccggtt gtaagaagga aacatttcct
      421 ccggagggta atctaattga gaactattct tttgaagaaa atggcgtatt tagcctaaat
      481 ggctgggaca cctccaatgc tcaatacagc acaattgtgc cggaaaacgg tggtgatttt
      541 tctgtaaaat tgtttccggg agcaccacct gatgaaggtt atgtggatta tatcataaac
      601 ggtttggtgg gtgaaaaaac gataactatg agttgccagg ctaatgcttt tggcggttgg
      661 gaaggtagtg caacactcaa attaattgct accggcgaaa caagtgctga tttagctacc
      721 gcaacctggg atcaaaatac ctggggcgca atagatttat ctgccactgc aacttttgtt
      781 gagggagatg tgttaatgct acatttaagt gccggattta gccctgtggt tgaaccggga
      841 aaatttgttt tatttgacct ggttacctta tccataaatt aactttaaat tgcttaagct
      901 tggcgagtat atttaatgca gaagatactg cagcaataat atcaaggatt gaaaaattaa
      961 ccccaattac acaacatatt tggggcacaa tgaccgtttc ccaaatgtta gcccatatac
     1021 aagaaccggt taaggttgcg cttggacata ccgcaccgaa acggtcggta atcggctggt
     1081 tatttggacg tgccgcaaaa aaaggtatta ttaatgaaaa accttttaaa caagggctac
     1141 caactgatag tagctttgta attgataatg aacgtaattt tgacactgaa aagcaggaag
     1201 ctattgaatt attgcgggca cttcaaaaag cgggtccaaa cggaataaca aaaaacaaac
     1261 atccattttt tggcaaatta tctgctgaag aatgggacac attaactata aaacatttgg
     1321 atcatcacct cagacagttt ggcgtataac caccattaaa tgacacaaaa aagttaccct
     1381 gttactaatt atcggctcat cttacttatc atcggcataa caggtttgct gatgcgtggc
     1441 tttttgggtg aaacagatgt tgttaccatt attttgttta ttattcaatt agttgcactt
     1501 gatttaatat taaaagaaag taatatcttt tctactgtct attatcgttt tattcaaatt
     1561 tgtatttcca tttatataat tggtgcctta tttatcatta tgcattggga aggaggaagt
     1621 ttactcttaa ccgcggctgt aagtggtgtt gttgttacct atatcatcag aacaattaac
     1681 aaatcgccaa taaatatttt agatatagta aaatgcgcct gggttatcag cgtgtgtttt
     1741 actaccattg ccaaattgat gcatttgcgg tatgctgata ttggtaatta tatcaattta
     1801 ggattatttg gggtgatgtt actattattt ttcctctctc ctgttaagcc tgcaccaaag
     1861 cataacaata gtcataacga actacccctg gatcaagtgg aataaattta tactttttcg
     1921 atcatgtgat agttatcaat tttatcgagc tcaaaaactg ttataccatc ttcattaaaa
     1981 taataaggca aatcaaattt agcatcccaa attatttcct gatgtgtata agaatttcta
     2041 gcataaatgt cctgaccgga agtttcatta tatcctttta ctagtattaa cacctcggct
     2101 tcacctattt ctacatcttg ttttgtcaat ccgtataatg ggcttttatc atcgattgga
     2161 tgcaccatgg tccagttaag cggaaagtac actattttat tattatcaat agtcagcggg
     2221 aaaaattttc tggttggttt accattttca tttattacca tagccaatgt tacacttaca
     2281 cttaattcag tgagattgtg attgcgttca tttgcggcgc gaaacattaa accgaaacct
     2341 tctttataag ggctgattaa agcatttgtt gaaaagcgga tgctatgtgg tgtttttgaa
     2401 aatctaccat acattaaacc ggtaaaaatg gcaaacccca ttaagccaac taaagcctca
     2461 cttgcagcta caaagctcac tgttgacgac aatggatata aagaaccata tccaacagta
     2521 gttaatgttt gagcactaaa aaataaggct acgaaaaatc tattatgaac ttcataatcg
     2581 gaagtcatgc ctataccatt ataatctacc gttaaataca tcatcgcaaa tattaaattt
     2641 acgatagaat aaaatgcaac aataaataag gcaaatttca accaactcat ggtaagtaac
     2701 tcatgaaaaa caagtttgcg ttcaccaatt ctccgcacat taaaattccc gtcatgagaa
     2761 agaatacgtt tatttccctg ttcgtatttg ttggtataac ctaattcatc agccttatcg
     2821 gtattaggat tgatattacg attcaatttt tccttgagtg ccatattgag ggtatttgca
     2881 aatgtggtaa atgcggggac taagctatta ttttatttcc tgtttatact aacgtttgtt
     2941 ggcataatcg gactaaaata aaaaaatccg gaatttagtt ccggatagta agtttattgt
     3001 gtggcgtagt tattgcctat ttttttcaga attct
//
LOCUS       DAORKW010000458         3030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1853_length_3030_cov_5.155966, whole genome shotgun sequence.
ACCESSION   DAORKW010000458 DAORKW010000000
VERSION     DAORKW010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1853_length_3030_cov_5.155966"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..486)
                     /locus_tag="PLB46_14065"
     CDS             complement(28..486)
                     /locus_tag="PLB46_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77698.1"
                     /translation="MDKIDFMDNLIKIILTCGSISLGALLGVLRSKIFASKENNKLHY
                     FFYGYSIVSALTCLIVSVMYWNQMDSFSIGVLIATFVLSILLWLATNKFLFFKNIFKS
                     TELDTPVNKFTSNADKNDIKLFGGDLNFFGNSPSEIDNNSQYVSSPFLVR"
     gene            complement(497..1006)
                     /locus_tag="PLB46_14070"
     CDS             complement(497..1006)
                     /locus_tag="PLB46_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77699.1"
                     /translation="MSDKREIVIKDACILFDLVDLNLLEDFFQLEVDAFTTSQVIGEI
                     TNEQQWDAVSRFINNGKLRIDDDGTFEVITKINEEYPGLSLPDSSVLELAIRKNAIVY
                     SSDGSLRKASLKEGITVRGIIWIIAELYRLELMTKESAIAKLNLYETINQRAPLKEIN
                     KLMDNLLNS"
     gene            complement(996..2072)
                     /locus_tag="PLB46_14075"
     CDS             complement(996..2072)
                     /locus_tag="PLB46_14075"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRF77700.1"
                     /translation="MEKVVENIFGNRLKLARKMAGFSLQELADALENKVSKQALNKYE
                     MGQMNPSSDVLVAISSTLKVKPDYFFKKQQVELGEILFRKKASLSKKSEDSIVEKVRD
                     YVERYLEIEGILEAATPFRNPLQNIKITKKSDVEVAANQLRKEWNLGTDPIPNIIEML
                     ELKGIKILLIDDVDDIDGLAVFTSTGIPVVVVNTRKKSIERIRFTITHELAHLLLKLE
                     KQILDNTKELEKYCHFFSSCFLIPTEMLLKQIGSGKRSYIRIEELIAIKEYFGISIRA
                     IVHRLKELEIITENYYHRWIVYMSKTYGAKEEPGVYKGEEKSRNFDQLVNRALAEELI
                     SLSKAAALWNVSVNELRKGFAGVR"
     gene            complement(2529..>3030)
                     /locus_tag="PLB46_14080"
     CDS             complement(2529..>3030)
                     /locus_tag="PLB46_14080"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRF77701.1"
                     /translation="ELEGASRPGHFAGVAQVVKLLLKITRPDILFLGQKDFQQCLILT
                     QLINSLKMPVELVMCPIYREPHGLAMSSRNVRLNPQIRQKAGAIYASLQYAAAMVNIL
                     TPQEIQQAATAALNTIEGFNVDYFLLLNADNLKPITPETPAHRIVIITAVIVDGVRLL
                     DNMVIS"
BASE COUNT          918 a          564 c          508 g         1040 t
ORIGIN      
        1 tttcaaataa ttaaaaagtc tattatatca acgtactaaa aatggggagc ttacatattg
       61 actattattg tctatttcag atggagaatt accaaagaag tttaagtcac caccaaacaa
      121 tttgatatca tttttgtccg cgtttgatgt aaacttattt acaggggtgt ccaattctgt
      181 ggatttaaaa atatttttaa agaataaaaa tttatttgtt gctaaccaca aaagaatact
      241 taacacaaat gttgcaatta aaacacctat cgaaaatgaa tccatttgat tccagtacat
      301 aacactaact attaagcatg tcaaagctga aacaatcgaa tagccgtaga aaaaataatg
      361 aagtttattg ttttcctttg aggcaaaaat cttacttcta agaactccaa gtaaagctcc
      421 aagtgaaata gatccacagg ttaaaatgat ttttattaaa ttatccataa agtcgatttt
      481 atccataaga ttatttttaa ctattaagta aattatccat taatttattt atttccttca
      541 aaggtgctct ttgattaatt gtttcatata aattcaactt tgctattgca ctttcctttg
      601 tcattaattc caatctatat aactcagcaa ttatccaaat aatacccctt actgttattc
      661 cttcttttaa agaagcctta cgtaaactgc catcagaact gtaaacaata gcatttttcc
      721 ttattgccaa ttcgagcaca gaactatcag gtaaacttaa tccgggatat tcctcattaa
      781 ttttggtaat tacctcaaat gtaccgtcat catcaatcct aagtttaccg ttattaataa
      841 atcttgagac ggcatcccat tgctgctcat tcgtaatttc gccaatcacc tgtgatgtag
      901 tgaatgcatc tacctctagt tgaaaaaaat cttccagcag attcaggtca accaagtcga
      961 ataggatgca ggcatcttta attactattt ctcttttatc tgacaccggc aaatcctttt
     1021 cttagttcgt taacacttac attccaaaga gcagcagctt tacttaaact tataagttct
     1081 tctgctaacg cccggttaac caattgatca aaatttctag atttttcttc acccttataa
     1141 actccgggtt cttctttagc tccgtaagtt ttgctcatat acacgatcca acgatgataa
     1201 taattttcag ttataatttc tagttctttt agtctgtgca caatagccct gatggaaata
     1261 ccgaaatatt cttttattgc aatcaattct tctattctga tatagctcct ttttcctgaa
     1321 cctatttgtt tcagcaacat ttcagttggt ataagaaaac agcttgaaaa aaaatggcaa
     1381 tatttttcta attcttttgt gttatcaaga atttgttttt ccagttttag caatagatga
     1441 gcaagttcgt gtgttatagt aaatcttatc cgctctattg atttttttcg ggtgtttaca
     1501 acaacaactg ggatgccggt agaagtaaat actgcaagcc catcaatatc atcaacatcg
     1561 tcaattaata atattttaat gccctttagc tccagcattt caataatatt aggaataggg
     1621 tcagtaccta aattccattc ttttcttaat tggttggcag caacttcaac atcgcttttc
     1681 ttagttattt taatattttg caatgggttt ctaaatggag tcgctgcttc aagaataccc
     1741 tcaatttcaa ggtatctttc aacataatct ctcacttttt ctacaattga atcttcactc
     1801 tttttcgaca ggcttgcttt ttttctaaaa agtatttccc caagttctac ttgctgtttt
     1861 ttaaaaaagt aatcaggctt aacctttagg gtgcttgata tggcaaccaa tacatcactc
     1921 gaaggattca tttgccccat ttcatactta ttcaaagctt gcttagatac tttattttct
     1981 aaagcgtcag caagttcttg aagggagaat ccagccattt ttctggctaa ttttagcctg
     2041 ttcccaaaaa tgttttctac taccttttcc atgattttag tttattgttg acaaaagtat
     2101 aaaaaaactt taaaatgtca accttatgtt gatgtttttt tagtctatat tctaattgaa
     2161 ttaagtcctt accttccgaa atattcaatg ggaaataaaa tgtttatgga attggatgtt
     2221 tgaattgaca ggttattaac gcagtgtagc caacaatata tttgcgaaaa ttcacaattc
     2281 acataatgcc actttaggtt caataaatca tattttatca atataaaact tatattttat
     2341 tagaaataaa aaatgatatg agtagcccaa aaccgcttta gccagttgta atagcgcctc
     2401 aaaagtttaa cctagcctgg ggcgcagcag aaagcccgca ggagccggaa cggagtggag
     2461 gtgacgagga cttggagcgg agaaccggtt gtgattttat tggcgtagat gatgttcatg
     2521 ctccaaattc aggatattac catattgtcg agcagtcgca cgccgtcgac tattacggct
     2581 gtgatgatga ctattcggtg ggcaggggtt tcgggggtta tgggttttag gttgtcggcg
     2641 ttgagtaata gaaaataatc aacattaaaa ccttcgatgg tgttgagggc ggccgttgca
     2701 gcttgctgga tttcttgcgg ggtaagtata ttgaccatag ctgcggcata ttgtaaactg
     2761 gcgtaaatgg ctcctgcttt ttggcgaatt tgggggttta ggcggacatt gcgtgaactc
     2821 attgccaagc cgtggggttc gcggtaaatg gggcacatca ccaattctac cggcattttg
     2881 agactattaa tgagttgcgt caggatgagg cactgctgaa aatctttttg tccgagaaac
     2941 aaaatatcgg gacgggtgat cttcaataat aacttaacca cctgggctac accggcgaaa
     3001 tgtcccggcc ggcttgcacc ttctaattca
//
LOCUS       DAORKW010000459         3019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1860_length_3019_cov_5.411943, whole genome shotgun sequence.
ACCESSION   DAORKW010000459 DAORKW010000000
VERSION     DAORKW010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1860_length_3019_cov_5.411943"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLB46_14085"
     CDS             <1..472
                     /locus_tag="PLB46_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77702.1"
                     /translation="YAISNTFEPSTKAVAVHAMVKGNKLGLMDGMSITALISLEDATV
                     DAVPINAIVNHEGQNYIFIVTDRHSEEEHHTEIETAEHEHDEHGHEHTEEDESVHTEK
                     GTTFEKIPVRKGTTDIGYAEIHLLKEIPADSKVVVNGAFFILAKMNNKGEAHAH"
     gene            485..856
                     /locus_tag="PLB46_14090"
     CDS             485..856
                     /locus_tag="PLB46_14090"
                     /inference="COORDINATES: protein motif:HMM:NF023903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3703 domain-containing protein"
                     /protein_id="HRF77703.1"
                     /translation="MKLYTSMPSSLKTFYNNELNQSANNFTIGDLQKSWFHLERAHIL
                     GQPYPIAHSFVHWKMLIFGFKTKNTKEIIGQIPRLLVGGIKSFVGIIPTGNTGGSNVP
                     PLQSMAISEDLLKVIKQNSKL"
     gene            853..2751
                     /locus_tag="PLB46_14095"
     CDS             853..2751
                     /locus_tag="PLB46_14095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRF77704.1"
                     /translation="MKLPFQDKKFLILLFSITIVVTLEVLSILGILIPMPYAPFVFAT
                     FILGIGYIVLWNGLKAIFKLKFSSINLLMTIAVCGAFYLGEYPEAAVVIVLYVLGERL
                     EDIGMENSKSALDELVSKAPKTAFVKSLNANVAIDNIEIGSIIQVKPGEMIPLDGKIF
                     SGETTVDEAAITGEPIPKDKRHGDYLFAGTLNKNGFIEIQTTKLSGETTFSKIIQLTF
                     EASTNKSDTQKFIQKFSKFYTPTIIAIALLVFMVPVFVFQLDLNHWLKQAITLLVIAC
                     PCALVISTPVAIYAAIGNASSKGALVKGGKYLEALANIKAIALDKTRTITFGNPIVTD
                     IIPLHGTSREELIACTAGAEIFSEHPLAHAIVDACRNEGFEPHETESFKSIAGKGAIA
                     KCLVCEDETIYIGKLDFIKEHQDTDAETVSIVEQLSLQGKTSVVVSFGAGVAGVIGLM
                     DEIKPDSIIALKQIQELHIESIMLTGDNEKAANYVAKIVGIKKIFGNMLPEHKSEKLN
                     DLLMQYRKVAMVGDGINDAPALALSTVGIAMGAAGSDTAIETANIALMNDKLSLIPFL
                     IRLSRKTLTRIKLNTIGAIGVKLIFILLALFGYGNLVLAIAADVGVTLFVIITSLNLM
                     KFEKETKY"
BASE COUNT         1055 a          529 c          552 g          883 t
ORIGIN      
        1 gtatgctatc agcaacacat ttgaaccaag cacaaaagct gttgctgttc acgcaatggt
       61 aaaaggaaac aaactaggtt taatggatgg gatgagtatt actgctttaa taagtttaga
      121 agatgcaact gttgacgcag ttccaattaa tgctattgtg aatcatgaag gccagaacta
      181 catttttatt gtaactgata ggcacagtga agaagaacat catactgaaa tagaaacagc
      241 agagcacgaa catgatgaac acggacatga acacacagaa gaggacgaat ctgttcatac
      301 tgaaaaaggc acaacttttg aaaaaattcc tgttcgaaag ggcacaacag atattggata
      361 tgctgaaatt catttattaa aagaaattcc ggccgacagc aaagtagtag tgaatggagc
      421 atttttcatt ctagctaaaa tgaacaataa aggcgaagca catgcacatt aaaattgtaa
      481 atcaatgaaa ttgtacactt caatgccttc gtcattaaag actttttata ataatgaact
      541 aaatcagtct gcgaacaact ttacaatcgg tgatttgcaa aaatcctggt ttcacttgga
      601 gcgtgcccat atattggggc aaccctaccc tattgcacac tcctttgttc attggaaaat
      661 gttgattttt ggttttaaaa ctaaaaacac aaaggaaata attggacaaa ttcctcgact
      721 attagttggt ggtataaaat catttgttgg tattatacct accggaaata caggtgggtc
      781 aaatgttcct cctttgcaat caatggcaat atctgaagat ttactaaaag ttattaaaca
      841 aaattcaaaa ctatgaagct tccatttcaa gataagaagt tcctaatact acttttctca
      901 ataacaattg tggtcacact tgaagtttta tcgattcttg gaattcttat accaatgcct
      961 tatgcaccat ttgtatttgc aaccttcatt ttaggaattg gttacatcgt gctatggaac
     1021 ggactaaaag ctatattcaa gctcaaattt agcagtatca atttattaat gaccatagct
     1081 gtttgcggtg cattttactt gggtgaatat cctgaagctg cagtagtaat tgttctttat
     1141 gttttaggag aacgtttaga agatatcgga atggaaaatt ctaaatctgc tttagatgaa
     1201 ttagtaagca aagcaccaaa gacagcattt gtaaaatcac taaatgcaaa tgttgctata
     1261 gataatattg aaattggctc tataattcag gtgaaaccgg gtgaaatgat accactcgac
     1321 ggtaaaatat tttccggcga aacaactgtt gatgaagctg caattacagg agagccaatt
     1381 cccaaagaca aacggcatgg cgattacctt tttgcaggaa cactaaataa aaacggtttt
     1441 atagaaattc aaacaacaaa actatccggt gagaccactt tctcaaaaat tatccaacta
     1501 acttttgaag catcaaccaa caaaagcgat acacaaaagt ttattcagaa attttcaaaa
     1561 ttttatactc caactattat tgcaattgct cttttagttt tcatggttcc tgtattcgta
     1621 tttcaattag atcttaatca ttggctgaag caggctatta cgcttttggt tatagcctgc
     1681 ccttgcgcat tggtaatatc tacaccagta gcaatctatg ctgcaattgg taatgcctct
     1741 tctaaaggtg cattagttaa gggtggcaaa tatttagaag cattggcaaa tatcaaagcc
     1801 attgctttag acaaaacacg aaccattaca tttggaaatc cgattgtaac agatattatt
     1861 ccgcttcatg ggacaagtcg tgaagaactt attgcttgca ctgcaggagc tgaaatattc
     1921 agtgaacatc cattagcaca tgcaatagtt gatgcttgtc gaaacgaagg atttgaacca
     1981 catgagaccg aaagttttaa aagtattgca ggcaaagggg caatagcaaa atgtttagtg
     2041 tgtgaggatg aaactattta tataggaaaa ttggatttca ttaaggagca tcaagatact
     2101 gatgcggaaa ctgtaagtat tgttgagcaa ctttcactcc aaggtaaaac cagtgtggta
     2161 gtaagttttg gtgctggagt tgccggtgtt atcggtttaa tggatgaaat aaaaccagat
     2221 agtataatag cattaaaaca aatacaagaa ctacacattg agtccatcat gttaactggt
     2281 gacaatgaaa aagcagcaaa ttatgttgca aagatagtag gtattaaaaa aatattcggc
     2341 aatatgttac ctgagcataa atctgagaaa ttgaatgacc ttttgatgca atacagaaaa
     2401 gtagctatgg ttggtgatgg aattaacgat gcacctgctt tagcactttc tactgttggt
     2461 attgcaatgg gtgcagccgg gagtgacacc gcaattgaaa cggctaacat cgctttgatg
     2521 aatgataaac tatccctcat tccatttctc attcgcctat caagaaaaac tttaaccaga
     2581 ataaagttga atactatagg tgcaataggc gtaaaattaa ttttcatttt gttggcttta
     2641 tttggttacg gcaatttagt attggcaatt gcagcagacg taggcgtaac tttatttgta
     2701 ataataacca gtttgaattt aatgaaattt gaaaaagaaa caaaatacta gttttaaaat
     2761 ttatatttac ttgacgaaac gcaattacaa ataaaaatat acttccctaa acacttaacc
     2821 aatccgcctc cccccgtccc accgaaatcc cctatcttta cccacataaa acaccatcca
     2881 tccatgctcc caacccagct caaaccaaaa caggcgctca acaaagcgtt tttgaaggtt
     2941 aaacctaaca ggaccgaaat tgaagggttt aaaagtaacc taattaccct gctggataga
     3001 acaaatgata ccgagagtg
//
LOCUS       DAORKW010000460         3019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1861_length_3019_cov_5.216262, whole genome shotgun sequence.
ACCESSION   DAORKW010000460 DAORKW010000000
VERSION     DAORKW010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1861_length_3019_cov_5.216262"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..2305
                     /gene="recG"
                     /locus_tag="PLB46_14100"
     CDS             179..2305
                     /gene="recG"
                     /locus_tag="PLB46_14100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRF77705.1"
                     /translation="MAILSLQTPLIYLKGVGEARAEQLRKELNLDNCLDLIQFFPYRY
                     IDRTDFKTVKQALELATKLEEVKDVQLKGRLKNIHVIGLKRAKRLVATLDDGTGSIEL
                     VWFNSISWLQKTLKPETQYLVFGKPYFFNGNLNITHPEIEDAATSEFQLSGKIQPMYP
                     GTEKLKARGINSKAIARYTRYLIDNLDEKELFEILPQPITDKYKLINRFEAYKQIHYP
                     DSEAHLQHAIRRLKFEELFIDQIKLLRVKQYRHSTSIGFILKNIEQHFTNFFNNHLPF
                     QLTEAQKRVLKEIRKDVLSGRQMNRLLQGDVGSGKTVVALLTMLMAIDNNFQSCLMAP
                     TEILAQQHYAGIKELLAPLNIRVELLTGSVKGKQRKIIFADLEKGEIPILIGTHALIE
                     DTVKFNNLGCVVIDEQHRFGVEQRAKLWAKNNIAPHILVMTATPIPRTLAMTYYGDLD
                     VSVIDELPPGRKPIKTVHRKESERLQVFGFLKEQIKEGRQVFVIYPLIEESEKLDLNN
                     LMEGIQAFERAFPRPQYHLSIMHGKMKPADKDFEMQRFVANNTQIMVSTTVIEVGVNV
                     PNATVMIIENAERFGLAQLHQLRGRVGRGAHQSFCILMTGDKLNNDARTRIQTMIQTN
                     DGFKISEVDMQLRGPGEIEGTRQSGAPNFKIANIATDEHIMREARLSAEDLLNEDGQL
                     EKPENMLLRKYLSEQKWKIKQWGKVS"
     gene            2514..2783
                     /locus_tag="PLB46_14105"
     CDS             2514..2783
                     /locus_tag="PLB46_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRF77706.1"
                     /translation="MNKGELIDAMAADTGLTKTQATACLDSMMTAIRKALKKGDKVTL
                     VGFGTYSVSKRAARTGRNPQTGKAIKIAAKNVVRFKAGKELSEAV"
BASE COUNT         1054 a          527 c          566 g          872 t
ORIGIN      
        1 ccaatgtgcg aatgtaccaa tgaggagcag ccgatattcg tctccgactt tttccctttt
       61 tccgcgtgaa cctggtttaa caacttagcg tgcttgaatt accacattaa tattaatatc
      121 caataccatc ccatttgcta atttgctaat taactaattt gctaattatc tttaccctgt
      181 ggcgatactg agtttacaaa ccccacttat ttatctgaaa ggagttggag aagctcgtgc
      241 cgaacaactt cggaaagaat taaatctgga taattgtctg gacttaattc aatttttccc
      301 ttaccgctac atcgaccgta ccgattttaa aacggttaaa caagcattag agctcgccac
      361 caaattagaa gaagttaaag atgtacaatt aaaggggcga ctaaaaaaca ttcatgttat
      421 aggattaaaa cgagcaaaac gtttagttgc tactttagat gatggcaccg gctctattga
      481 attagtttgg tttaacagta tcagctggtt acaaaaaacg cttaaaccgg aaacacaata
      541 tttagtattt ggaaaaccgt atttctttaa tggcaattta aatatcaccc accccgaaat
      601 tgaagatgct gccaccagtg aatttcagct aagtggtaaa attcaaccta tgtatcccgg
      661 cacagaaaaa ttaaaagcca ggggtattaa tagtaaagct attgcaagat acacgcgcta
      721 tttaatcgac aatctggatg aaaaagaatt atttgaaatt ttacctcagc ccattaccga
      781 taaatataaa ttaataaaca ggtttgaagc gtataaacaa attcactacc ctgattcgga
      841 agcacatttg caacatgcca tccgcaggtt aaaatttgaa gaattattta ttgatcaaat
      901 aaaattatta cgggttaaac aataccgaca cagcaccagt atcggtttta ttttaaaaaa
      961 cattgagcaa cattttacca atttttttaa taatcattta ccatttcaac taacagaagc
     1021 acaaaaacga gtattaaaag aaataagaaa agacgtactc agtggccgtc aaatgaacag
     1081 gcttttacag ggtgatgttg gcagtggaaa aacagtagtt gcattattaa ctatgttaat
     1141 ggccattgac aataatttcc aaagttgttt gatggcacca accgaaattt tggcacaaca
     1201 gcattatgcc ggaataaaag aattattagc accactcaat attcgtgtag aattattaac
     1261 aggaagtgta aaaggtaaac aacgaaaaat tatttttgct gatttggaaa aaggtgaaat
     1321 tccaatttta attggcacac atgcattaat tgaggatacc gttaaattta ataatctggg
     1381 ttgtgtagtt atagatgaac aacatcgttt tggtgttgaa cagcgtgcca aattgtgggc
     1441 taaaaataat attgcgccac atattttagt gatgactgct acaccaattc cacgtacgct
     1501 tgccatgacg tattatggtg atttagatgt gagtgtaatt gatgagttac ctccaggccg
     1561 aaaaccaatt aaaacagttc atcgaaaaga aagtgaacgt ttgcaggtgt ttggtttctt
     1621 aaaagaacaa ataaaagaag gcagacaagt ttttgtgatt tatccattaa ttgaagaaag
     1681 tgaaaaactt gatttaaata atttaatgga gggcattcag gcatttgagc gagcattccc
     1741 acgaccgcaa tatcacttaa gtattatgca tggtaaaatg aaacctgcag ataaagattt
     1801 tgagatgcag cgttttgtcg ccaacaatac acaaataatg gtatcaacaa cggtgataga
     1861 agttggtgta aatgttccca atgcaactgt tatgattatt gaaaatgcag aacgatttgg
     1921 attagcacaa ttacatcaat tacgaggccg tgttggaaga ggtgcacatc agtcgttttg
     1981 tattttaatg accggcgata aattgaataa cgatgcacga acacgtattc aaacaatgat
     2041 acaaaccaac gatggtttta agatttcgga agtagatatg caattgcgtg gtccgggtga
     2101 aattgagggc actagacaaa gtggtgcgcc taattttaaa attgccaaca tagccacaga
     2161 tgaacacatt atgcgtgaag cccgcttgtc cgccgaggac cttttgaacg aggacgggca
     2221 gctcgaaaaa ccggaaaaca tgcttcttcg caaatattta tcagagcaaa aatggaaaat
     2281 aaagcaatgg gggaaagttt catgaaaaaa atatttgctc attatttgcc ttttttacta
     2341 taaataggtg ccaaaacagg ctaaaaccct tcaaatacga gggtttcagg ctaacaatgt
     2401 gtataaagtg ttgaaaatac aggggttcca gcgaattaat attttgaaat gcccgtaaac
     2461 actgtatttt tggcactgta aaacttattt aataactaat aaaacaaatt acaatgaaca
     2521 aaggcgaatt aatcgacgca atggcagccg acacaggctt aaccaaaact caagcaactg
     2581 catgcttaga tagcatgatg actgcaatcc gtaaagcatt aaaaaaaggt gacaaagtta
     2641 ccttagtagg tttcggaact tactctgtaa gcaaacgtgc tgctcgtacc ggccgtaacc
     2701 cacaaactgg taaagcaatc aaaattgctg ctaaaaatgt ggtacgtttc aaagcaggta
     2761 aagaattatc tgaagctgtt taataaaaca tttctcaatg aggaaaacat ccgcagttgc
     2821 gggtgttttt ttttgcctga aactttgaaa tattagtgta caatttcttt cacaaattgc
     2881 atatcataca atttgcgata gtaaccattc aattcgagta attgagtatg tgttcctatt
     2941 tcttttactt cgcctttatc taaaactaaa atctggtcgg ctcgctgtat agtgctcaac
     3001 ctgtgagcaa ttatgattg
//
LOCUS       DAORKW010000461         3015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1864_length_3015_cov_4.798986, whole genome shotgun sequence.
ACCESSION   DAORKW010000461 DAORKW010000000
VERSION     DAORKW010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_3015_cov_4.798986"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..1118)
                     /locus_tag="PLB46_14110"
     CDS             complement(294..1118)
                     /locus_tag="PLB46_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF77707.1"
                     /translation="MAQQLLKGKRGIIFGALDESSIAWKVAEKCFEQGATFTLTNAPI
                     AMRMGAINQLAEKCNAEIIPADATSIEDLEKLISRSVEVLGGKIDFILHSIGMSPNVR
                     KNRAYTDLSYEFLMKTLDVSAVSFHKVLQTAYKQDAISEWGSVLGLTYIAAQRTFPSY
                     SDMAEAKATLESIGRSFGYHYGKHRKVRVNTISQSPTWTTAGSGVKGFDAFFNYADKL
                     SPLGNASADACADYCVFMFSDLSRMITMQNLYHDGGFSSVGVSQEIMDMLMGDSEA"
     gene            complement(1172..1693)
                     /locus_tag="PLB46_14115"
     CDS             complement(1172..1693)
                     /locus_tag="PLB46_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77708.1"
                     /translation="MKNKYFIFIAMCLISVSLQAQTGKGNRKQYYVLITDTTFVQAIP
                     DQGATFKAYFKNDSLYKIETWFGFNFGDVKREYFYWNDSIKLINEYQKLYNSLGNPKP
                     NPDTIKVTYTGRYIFKHNVLTDISQKGSYSISDTPQGKQEIEAGFLMLTEKYKKLAYD
                     KRKKKKNRIKFTE"
     gene            1748..2356
                     /locus_tag="PLB46_14120"
     CDS             1748..2356
                     /locus_tag="PLB46_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRF77709.1"
                     /translation="MALTRTNHSGEKPLLKNPAIIVVNFLLVGIVVLFLSFSYGYFAF
                     KNDKWLDFRLPRVFWISAMLVVAISVVMRNMIRYYDADNHIKLRRNILVALALALGFV
                     ICQFIGWQNMQQQGMLLDTTTGASYVYLLTGLHAIHILLGIIFLSVATYRIFLNTRNQ
                     VNALIYFSDPLHRSRIKMMATYWHTIDALWIYLFLAFLYNHT"
     gene            complement(2445..2840)
                     /locus_tag="PLB46_14125"
     CDS             complement(2445..2840)
                     /locus_tag="PLB46_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77710.1"
                     /translation="MKKIMVMMTVALMVFSTNMFAQTATGDAKVATEKKEKIKAAPIS
                     AKKSTVIKNGNKTGEDKKAVNNHKDELKAKYDSKAEAKEAVKAHQGDKKVSDKDRVKA
                     HKSDIKTKADSKQEAKAKLQRKHAKKDDE"
BASE COUNT          862 a          551 c          504 g         1098 t
ORIGIN      
        1 acgcccgggt atgccgcgca catttaatgt gggattaaaa ctggatattt aattagcatg
       61 gcaaatgata attacctgaa ttaatatatt actactttac ttacctttta caattacatt
      121 ttgttctgtt aaaacaataa aataaaggat gtatgttttc taaaaaaaaa cataatacaa
      181 ataaattaca tggtttatca ttaaaatgtt taagcataat ttctgtaatt aaaagtacat
      241 aaaaaaacga ggtataaaat gatacacctc gttttttata ttataaattg aatttatgct
      301 tcgctatcgc ccatcagcat atccataatt tcctgactaa cacctactga actaaatccg
      361 ccatcgtgat ataggttctg catggtaatc attctactta aatcgctgaa cataaataca
      421 caataatcgg cacaggcatc agcgctggca ttaccaagtg gagataattt atcagcataa
      481 ttaaaaaatg catcaaaacc tttaactcca cttcccgcag ttgtccatgt tggtgattgt
      541 gaaatggtat taacgcgtac tttacggtgt ttaccataat gatagccaaa gctgcgacca
      601 atactttcaa gtgttgcttt tgcttcagcc atatcgctat aacttgggaa tgtacgttgt
      661 gctgcaatat aggttaaacc taaaacactt ccccattcgc tgatagcatc ttgtttataa
      721 gcagtttgta atactttgtg aaatgaaaca gcagatacat ctaatgtttt cattaaaaat
      781 tcgtaactca aatcagtata tgcacgattt ttacgcacat ttggactcat gcctattgag
      841 tgtaaaataa aatctatttt accgcctaaa acttctacgg aacggctaat taatttttcc
      901 aaatcttcaa tgcttgttgc atcggccgga ataatctcag cgttgcattt ttcagctaac
      961 tgattaatag cacccatacg catagcgata ggggcgttgg taagtgtaaa tgtagcacct
     1021 tgttcaaaac atttttctgc aaccttccat gctattgaac tttcatctaa ggcgccgaaa
     1081 ataattcctc tttttccttt taataattgt tgagccatat aattggtttt aagtgattgt
     1141 gttattgcga aaatacaaag ttataaatag tttactctgt aaacttaata cggtttttct
     1201 tcttcttgcg cttatcgtaa gccagttttt tatatttctc agttagcatt aaaaatccgg
     1261 cctcaatttc ctgtttaccc tgaggtgtgt cggaaatact gtaactgcct ttttgggaaa
     1321 tatcagtcag cacgttatgt ttaaaaatat agcgacccgt ataggtaact ttaatcgtat
     1381 ccgggtttgg ttttgggtta cctaatgaat tatataattt ttgatattca ttaatgagtt
     1441 taatggaatc gttccaatag aaatattccc gttttacatc accaaaatta aaaccaaacc
     1501 aagtttcaat tttatagagt gaatcgtttt taaagtaagc tttaaatgtt gctccttgat
     1561 ccggtatagc ctgaacaaaa gttgtatcag ttatcaatac ataatactgt ttgcggtttc
     1621 ccttacctgt ttgcgcctgt aaagagacgg aaatgaggca catagcgatg aaaatgaagt
     1681 atttattttt catatatctg aaaacaaagt tagtagataa attgttacca agctgtaatt
     1741 ttgccttatg gcgctaacca ggactaatca ctccggcgag aaacctctcc tcaaaaaccc
     1801 tgctattatt gtggtaaatt tcctattagt aggcattgta gtgctttttt tgtccttttc
     1861 ctatgggtat tttgcgttta aaaatgataa atggctggat tttaggctcc caagggtgtt
     1921 ttggattagc gcaatgctgg ttgtggcaat cagcgtggtt atgcggaata tgattaggta
     1981 ttatgatgca gataaccata tcaaactccg caggaacatt cttgtggcat tggcattggc
     2041 acttggtttt gtgatatgcc aatttatcgg ctggcaaaat atgcaacaac agggtatgtt
     2101 gcttgatact acaaccggtg cctcgtatgt gtatttgctc accggattgc atgccataca
     2161 cattttgtta ggcattattt ttttaagtgt ggcaacatat cgcatttttc tcaatacccg
     2221 caatcaggtt aatgcattaa tctacttttc tgacccgctt catcgcagtc ggattaaaat
     2281 gatggcaacc tattggcata ccatcgatgc gctttggata tatttgtttt tggctttttt
     2341 atacaaccat acctaattat tgctttagtg atttgccaca aaaaataacc ccctcagttt
     2401 tcaccgaagg ggtttgtttc actccttaac caactatatt atatttactc gtcgtctttt
     2461 ttagcgtgtt tgcgctgtaa tttagctttt gcttcttgtt tgctgtcagc tttagttttg
     2521 atgtctgatt tatgcgcttt aacgcgatct ttatcactta cttttttatc accttgatga
     2581 gcttttacgg cttctttagc ttctgctttt gaatcgtatt ttgcttttaa ttcatctttg
     2641 tgattattta ccgctttttt atcttcacct gtcttattgc cgtttttaat tactgttgat
     2701 ttttttgctg aaatcggagc ggctttaatt ttttcctttt tttcagttgc tactttagca
     2761 tcgcctgttg cagtttgtgc aaacatgttg gttgaaaaca ccatcaaggc cacggtcatc
     2821 attaccatta tctttttcat aattcttagt tttaatagtt aatcaatgtt tgatggcaat
     2881 aatacgaata ccatgccaaa gttgttttat gtgccgagta aaacttaacc ggaagggact
     2941 agtttttact ctaaactact cattttcaac aagctacaac aattcgtttt aaatttcagt
     3001 tttggtttat acatt
//
LOCUS       DAORKW010000462         3012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1868_length_3012_cov_3.726412, whole genome shotgun sequence.
ACCESSION   DAORKW010000462 DAORKW010000000
VERSION     DAORKW010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1868_length_3012_cov_3.726412"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLB46_14130"
     CDS             <1..292
                     /locus_tag="PLB46_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRF77711.1"
                     /translation="TYEWEVAVPGMKKEDFKVEVKDGMLSIYAERADEKMEEEKNYTR
                     KEFSCMSFSRMFTLPENVKDGEIDATYVNGVLTIVIPKTSIAVAPVKKVEVK"
     gene            complement(354..1076)
                     /locus_tag="PLB46_14135"
     CDS             complement(354..1076)
                     /locus_tag="PLB46_14135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRF77712.1"
                     /translation="MLKNVNPEALFFFDIETVPGNPDYHELSPDFRKLFDDKVGRFKK
                     EEENPADYYFSKAGILAEFGKVIVISCGYLQKKGEAYILQIKTFSNHDEKLLLESFLP
                     TLINVSKNDYQLCGHNIREFDVPWLCRRFLINGLPLPPVLDIYGKKPWEVNHVDTLDL
                     WKFGDFKHYTSLHLLANVLNVPTPKDDIDGSMVGAVYWKENNLSRIAKYCSKDVIAVA
                     QIIMRLKGLPLINDENIVFQET"
     gene            complement(1118..1819)
                     /locus_tag="PLB46_14140"
     CDS             complement(1118..1819)
                     /locus_tag="PLB46_14140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF77713.1"
                     /translation="MAKTIIITGADGGLGSAVTAELLKNGHQLIASAVSQKSIDKLQS
                     LFPEAFGKQLKAVIADLSDESAIDNLFHQGENIGGLVHLAGGYTAGASIADYSLQDYQ
                     HLMNLNVLPTFLLLKKIVPVLKTNGGGSIVTVGAKPAIHQSAGNAVYAASKSAVITLT
                     LSTAEEGREFNIRANCIAPATLQTPNNLSWATPEQFKTFTPITDVAAVIAFLQSDEGV
                     GINGMVIPMYNKLPA"
     gene            1970..2623
                     /locus_tag="PLB46_14145"
     CDS             1970..2623
                     /locus_tag="PLB46_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77714.1"
                     /translation="MKKLTLTLLVTIATLFSARTSFAQLWVMGDVGGFLTEDLIGYGV
                     NARGAFHFGDYDRNMATLGGGFNMLPNETWQFVVYDSTGLFTDTVPSVLKANVISIDA
                     DYRRYFFQTDADDYFAFYGLVGASLWMVNSKMSLGTYVDTIFAPPLGTPLGQSNMSVR
                     IPVGFGVDWTVRGRFWWYFEAKTEIPVTQVNNDFIGNDFGMSYHINTGVRILLWDIY"
     gene            complement(2624..>3012)
                     /locus_tag="PLB46_14150"
     CDS             complement(2624..>3012)
                     /locus_tag="PLB46_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF77715.1"
                     /translation="AIGDIALMQADSAFPNGHPGVAQVAMQQADNLVDNFKRLHKNKS
                     LQPFIYRNKGNMATVGRHRAVVDLPFYSFGGYFAWYIWMFIHLMSLVGFRNRFMVFIN
                     WMWNYITYDRALRIIINNTGNTKQTG"
BASE COUNT          893 a          551 c          591 g          977 t
ORIGIN      
        1 aacgtatgaa tgggaagttg cggttcccgg tatgaaaaaa gaagatttta aagttgaagt
       61 aaaagatggt atgctttcca tttatgcaga acgcgccgat gaaaaaatgg aggaagaaaa
      121 aaactataca cgtaaagagt ttagttgtat gtcattctcc agaatgttta cgcttcctga
      181 aaatgtaaaa gacggtgaaa ttgatgctac atatgtaaat ggagtactta caattgttat
      241 ccctaaaaca agcatagcag ttgcacctgt aaaaaaggtt gaagtgaaat agataatcag
      301 gtttttatca gtacttagtt ttagaaatgc ccggtggttt gccgggcatt tttttatgtt
      361 tcctgaaaaa cgatattttc atcgttgatt aatggtaatc cttttaatcg cataataatt
      421 tgtgcaacgg caataacatc tttactacag tattttgcaa ttctggataa attattttct
      481 ttccaataaa cggcacctac catactacca tcaatatcat cttttggtgt tggcacattt
      541 aacacattgg ctaataaatg taaagaagta taatgtttaa aatcaccaaa tttccataag
      601 tcgagtgtat ctacatgatt tacttcccat ggttttttac cataaatatc taataccggg
      661 ggtagtggta atccgttaat taaaaaccgt cggcataacc atggtacatc gaattcccga
      721 atattatgtc cgcacaattg gtaatcgttt ttggagacat taatcagggt tggtaaaaac
      781 gattccagca ataatttttc atcgtgatta ctaaatgttt taatctgtaa gatgtaggct
      841 tcgccttttt tttgcaggta tccgcaggat attacaatca cttttccaaa ttcagctaat
      901 atgccggctt tagaaaaata ataatctgca gggttctctt cttctttttt aaaccgtcca
      961 actttgtcgt caaaaagttt tcgaaagtca ggggacaatt catggtagtc tggattgccc
     1021 ggtactgttt cgatgtcgaa aaagaacagg gcttcgggat ttacattttt aagcataatt
     1081 aagggaatta caatttttgg gtgaaaataa ataaaaatca ggcaggtagt ttattataca
     1141 ttggaattac cattccattg atgccaactc cttcgtctga ttgcagaaat gcgattactg
     1201 ctgctacgtc tgtaattggt gtaaatgttt taaattgttc aggtgtagcc caacttaaat
     1261 tattgggtgt ttgtaatgta gccggtgcaa tacaatttgc gcggatatta aattcccgac
     1321 cttcttcggc agtgcttaag gtaagtgtaa taacggcact tttgctcgcg gcataaacag
     1381 cgttccctgc actttgatga atggcaggtt ttgcgccaac tgtaacaatg gagcctccgc
     1441 cattagtttt taataccgga acaatctttt ttagcagtaa aaatgtgggc aaaacattaa
     1501 ggttcatcaa gtgttggtaa tcctgcaaac tgtaatcggc aattgaagct cctgccgtgt
     1561 aaccaccggc taaatgcacc aaaccaccaa tgttttcgcc ttgatgaaac aaattatcta
     1621 tcgccgattc gtccgataaa tcagctatta cagcctttag ttgctttcca aaagcctcag
     1681 gaaacaggct ttgaagttta tcgatacttt tttgggatac cgcactggct atcagctggt
     1741 gaccattttt cagcaattcc gctgttacag cgcttcccag cccaccatcg gcaccggtaa
     1801 taataattgt tttagccatt tttttatacc aaaattaagc tattgtcatt tgtggaggac
     1861 aatttcaggc ttgttgtgca gatagaattt taaattgcaa tcaaaaaacg tttattttgt
     1921 gcagtattgt gcagaaaact gccccttaca gctaatcaat atcttgagta tgaaaaaatt
     1981 gacacttaca ctgcttgtta caattgcgac tttattttct gcacgaacat catttgctca
     2041 gctttgggta atgggtgatg taggcggttt tttaacggag gacttgatag gttatggggt
     2101 aaacgcaaga ggtgcctttc attttggtga ttacgaccgc aacatggcta ctttgggcgg
     2161 tggtttcaat atgttaccta acgaaacatg gcaatttgtt gtttatgatt caacaggatt
     2221 gtttaccgat acggttcctt cagtactaaa agcgaatgta atatcaatag atgcggacta
     2281 tcgcagatac tttttccaaa ccgatgccga tgattatttt gcgttctatg gtttagttgg
     2341 tgccagcttg tggatggtaa attcgaaaat gagcttaggt acttatgtag ataccatatt
     2401 tgctccacca ctaggaactc ctttaggaca atctaatatg agcgttagaa tcccggtagg
     2461 atttggtgtt gactggacag ttcgcggccg tttctggtgg tattttgaag ccaaaactga
     2521 aattcctgta actcaggtga acaacgattt tatcggtaac gattttggta tgagttatca
     2581 tatcaatacc ggtgttcgta ttcttctttg ggatatttat taattatcca gtctgtttag
     2641 tatttcccgt attattaata ataatgcgta gtgctctgtc gtacgtaatg taattccaca
     2701 tccagtttat aaataccata aatcggttgc gaaatcctac taatgacatt aaatggataa
     2761 acatccaaat ataccaggca aaataaccgc cgaaggaata aaagggtaaa tcgacaactg
     2821 cacgatgtct tccaactgtt gccatatttc ctttattgcg ataaataaaa ggttgcagcg
     2881 atttattctt gtgcaaacgt ttaaaattgt caaccaaatt atctgcctgt tgcatcgcca
     2941 cttgcgctac tcccggatgc ccgttgggaa aagcactgtc agcctgcatt aacgcgatat
     3001 caccaatagc at
//
LOCUS       DAORKW010000463         2999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1877_length_2999_cov_6.670856, whole genome shotgun sequence.
ACCESSION   DAORKW010000463 DAORKW010000000
VERSION     DAORKW010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1877_length_2999_cov_6.670856"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLB46_14155"
     CDS             <1..340
                     /locus_tag="PLB46_14155"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF77716.1"
                     /translation="QVVHHCADSHMNSIIRLKLALTEDKPTIKAYNESAWAKMADSIT
                     FPIHDSLQIIKGVHHRWVAVLHAMNEADFQKTFIHPASGKEITLFVNTALYAWHCEHH
                     LAHINIVANA"
     gene            complement(345..962)
                     /locus_tag="PLB46_14160"
     CDS             complement(345..962)
                     /locus_tag="PLB46_14160"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HRF77717.1"
                     /translation="MMKQNYTVHPWHGISAGVNAPDAVTAFIEIVPTDTVKYEIDKAS
                     GFLKVDRPQKYSNVLPCLYGFIPQTYCGQKIGDYCSSKTGRTGIYGDGDPLDICVFTE
                     KNITHGNIIVPATPIGGFRMIDGGQADDKIIAVMYHDEIYSGWDDIDHMPLSLVERLK
                     HYFLTYKAMPGAPNKAEITHLYNKQEAREIIKASMEDYFSLIKSE"
     gene            complement(973..1746)
                     /locus_tag="PLB46_14165"
     CDS             complement(973..1746)
                     /locus_tag="PLB46_14165"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRF77718.1"
                     /translation="MSASALNAYLDCPVKYYFERIVGVPAAKNDSMSFGSAVHYALRR
                     MFERMREAEGHPFPTIHVVLSDFLYEMRRNEDSFTEKQFQNRLDLGKQVLTDYYNKYI
                     NHFNKVVVLEYPIRNVIMDDVPITGVFDKVEFNGNDVNVVDYKTGNADRSKEKLYPPH
                     PKDPNGGDYWRQIMFYKILLDNQKYKPWKMVSGEIDFLEKNEKGDLVKYRFPIVQDEV
                     DVVKAQIKEVYHQIKSLAFTEGCGKEDCKWCNFVRETAQ"
     gene            complement(1903..2913)
                     /locus_tag="PLB46_14170"
                     /pseudo
     CDS             complement(1903..2913)
                     /locus_tag="PLB46_14170"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
BASE COUNT          867 a          611 c          492 g         1029 t
ORIGIN      
        1 acaagtagtg catcattgcg ccgacagtca tatgaatagt attattcgac taaagctggc
       61 cttaacagaa gataaaccca caataaaagc ctataacgaa tcggcttggg cgaaaatggc
      121 agatagtatt acttttccaa ttcacgattc tttgcaaata atcaaaggtg tgcatcatcg
      181 ttgggtagcc gttttacatg ctatgaacga agctgatttt caaaaaactt ttattcatcc
      241 ggcttctggt aaagaaatta ctttgtttgt taatacagca ttatatgcat ggcattgtga
      301 acatcacctt gcgcatatca atattgtggc aaatgcgtaa ataattattc agattttatg
      361 agactgaaat aatcttccat tgaagcttta attatttcac gcgcttcctg tttattatat
      421 aagtgtgtaa tttctgcttt attgggcgct ccgggcatag ccttgtaggt taaaaaataa
      481 tgttttaaac gttcaaccag cgacaatggc atgtggtcaa tatcgtccca tccggaataa
      541 atttcgtcgt gatacattac ggcaataatt ttatcatcag cctgacctcc gtcaatcatc
      601 ctgaatccgc ctattggtgt ggccggaact ataatattgc catgtgtgat atttttttcg
      661 gtaaacacac atatatccaa cgggtctcca tctccatata ttccggtacg tcctgttttt
      721 gatgagcaat aatcaccaat tttctgacca caataagttt gcggaataaa tccatataaa
      781 caaggtaata cattgctgta tttctgtggc cggtcaacct ttaaaaatcc gctcgcttta
      841 tcaatttcgt actttacagt atccgttggc actatttcga taaatgccgt aacagcatcg
      901 ggtgcgttaa ctcctgcgct tatgccatgc caagggtgaa cggtataatt ttgtttcatc
      961 atgttattca tattattgtg cagtttctct tacaaaattg caccatttgc aatcctcttt
     1021 tccgcaacct tcggtaaatg ctaaactttt aatttgatga tatacttctt ttatttgtgc
     1081 tttcaccaca tctacttcat cctgcacaat tggaaaacga tattttacta aatcaccttt
     1141 ttcatttttt tcaagaaaat caatttctcc acttaccatt ttccaaggtt tatatttttg
     1201 attgtcgagc aaaattttat agaacataat ttgtcgccaa taatcaccgc cattcggatc
     1261 ttttggatgt ggcggatata acttttcttt acttctgtcg gcattaccgg ttttgtaatc
     1321 gacaacgtta acatcattcc cgttaaattc aactttatca aacaccccgg ttattggcac
     1381 atcgtccatt attacattgc gaataggata ttccagcacc actactttat taaaatgatt
     1441 gatatacttg ttataataat cagtcaatac ctgtttacct aaatcgaggc gattttgaaa
     1501 ttgtttttcc gtaaatgaat cctcgtttct gcgcatctca tataaaaaat cgctaagcac
     1561 aacatgaatg gttggaaatg gatgaccttc tgcttcgcgc atgcgttcaa acattcttct
     1621 caatgcataa tgcacggcag aaccaaatga catactatcg tttttggcag caggtacacc
     1681 aacaattctt tcaaaataat attttaccgg gcaatctaaa taggcattta aagctgaagc
     1741 gctcatcaca aaatgttcta ctttttgttg gatatactcc ttgtcaaaca aggtaatcat
     1801 caccttaggc gtttccatta aagcggtgat tttatattcc gccaacagct cctctgcaat
     1861 ggttttgggc tgttgaatta atcccgtgcc ttctttcaat tcggctataa atgcagatgg
     1921 ttcagcatct ttaccctcat tggtatgtga agcgtaggaa atatataaat gttgtttcgc
     1981 tctggtcatt gccacataaa acaaacggcg cagtccttca attttattgc tgctttcggt
     2041 aaatgtcaag gtatcgggca acacaaattt ttgattgttt gctctcgact tttcccattt
     2101 atcactattg caaccaatta aaaatacata ctcaaattcc aatcctttac tggaatgggt
     2161 agtaataaaa tttacgccgc cggttttaga aatagatttt tgtatttcta tttttaaatc
     2221 gtgtacctgc atttgctcca atgtatctaa atattgatga atatttaatc tcggcaaacg
     2281 caaaccttcc gatttaatat ggtcaaataa ggtggtaatt acttctaaat taaataccct
     2341 gttttctcca cgtaataaat actgcaacat gccactttcg tttaacactt tttcaaataa
     2401 catttgcagt gtcaggttaa aagactcaga caaccagtgg gttaattttt cttcaaactt
     2461 atcaatggct tcgtgactgc gcagtttaat cgtatttaat accgatttat ctgccaaaac
     2521 cgagcgccag gtaaatgttc tgtctcgttt tgcactaatc catgcagaaa ttgcagcaac
     2581 atcatgcgga tgtataccaa aaaacggata atgcattatt tcaaataata aatattcacc
     2641 gctatgtggt tttttacttt catcacccag gtaagttaaa atattaatca tttgatttac
     2701 cagcggtaat tccaaaatat ttatttttct acgcacctga taaggcaaac ctcttttttc
     2761 aaaaacgttg atgagttctt ccgcttgtgc gtgtttatga tataaaactg caatttcctc
     2821 tgcaggaaca ccattattta ataaagcctc aattgccatc aacacgcccg catgctcctg
     2881 tgccggattt tggtattcaa taatggcagg ggccggacta tgttttcttg ctggagggtc
     2941 cgcggggagg gaccttgatt aattttttta aatattgaac tctcgatgtt tctcttttt
//
LOCUS       DAORKW010000464         2992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1882_length_2992_cov_3.790943, whole genome shotgun sequence.
ACCESSION   DAORKW010000464 DAORKW010000000
VERSION     DAORKW010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1882_length_2992_cov_3.790943"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLB46_14175"
     CDS             complement(<1..259)
                     /locus_tag="PLB46_14175"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRF77719.1"
                     /translation="MTVSFRDSKSESKCYFVTTSCYKHIPLIALCNGFNHVANSVNKY
                     TTKYDADVLSYVIMPNHLHLLLYFKQEVRLSNLMRDIKKQSS"
     gene            complement(431..1717)
                     /locus_tag="PLB46_14180"
     CDS             complement(431..1717)
                     /locus_tag="PLB46_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF77720.1"
                     /translation="MVIIIGLMFLSACSVIYPPRTTPIPPIEVKTEPVDQNTTTTETK
                     TGNEKAKDSVATSVPLKSWAKKDRYTISFVLPFSTDKAELLKLMGEDKITGYQPLASL
                     EFYEGALIALDTLERLGIKLNVQVFNHLKDSLSTAMLLQKEEIKKSDVIIGPVFNESL
                     KAAAPIAKKQEVYLVSPLSPSNTFADTNKYFVMANPAITTQLSSMVDYVLDNNVAANF
                     IVVYRNDKPAEMKIAEEFKAAFNAQKGSSAATLKEVNNFAGISSNINGGGNFVFIAGN
                     DELYINGLIRDLSKIGRNNDITLIGLQNILSLESVSLDYFESLHFHYPTGYYVDQKLP
                     QVKEFNAAFEARYSTRPSDYAYRGYDVMMYFGTMLNTYGPDLSLSGGKVNPTLRYMMY
                     PLEFKAVKNAGGVIEHIENAKITILKYEDYRFQKAN"
     gene            complement(1725..>2992)
                     /gene="guaA"
                     /locus_tag="PLB46_14185"
     CDS             complement(1725..>2992)
                     /gene="guaA"
                     /locus_tag="PLB46_14185"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRF77721.1"
                     /translation="VQPSSKREYGRAYLHILERNNNLFYGVDDESQVWMSHSDTVTQL
                     PEGFHITAKTASIDVAAYASNPGRFEYPVFCIQFHPEVTHSEQGKTMLENFVVQICGC
                     KQTWTPESFVEETITHLQNTIQQDKVVLGLSGGVDSSVAAILLNKAIGSNLISIFVNN
                     GLLRKNEFDTVLHSYKDMGLNVVGVDVADEFITALAGVSDPEKKRKIIGKVFIDVFER
                     ESKKLEGVKWLAQGTIYPDVIESVSVHGPSATIKSHHNVGGLPEKLHLKVIEPLRYLF
                     KDEVRRVGDTLQLKHEILHRHPFPGPGLAIRIISDITAEKVRITQEADAIFINHLKQS
                     GWYDKVWQAGAILLPVQTVGVMGDERTYENVIALRCVNSSDGMTADWSHLPHELLAQI
                     SSDIINHVKGINRVVYDISSKPPATIEWE"
BASE COUNT          944 a          602 c          497 g          949 t
ORIGIN      
        1 atgatgattg ttttttaata tcccgcatta aattacttag ccggacttcc tgtttaaaat
       61 agagtagtaa gtgcaaatga ttaggcataa taacatacga taaaacatca gcatcatact
      121 ttgttgtata tttatttact gaatttgcga catgattaaa tccgttgcat aacgctatca
      181 acggaatatg tttgtaacaa gaagtagtta caaagtaaca ttttgattcc gatttggaat
      241 cacgaaaaga aacagtcatt aatttgaatt taagggtaaa acaataggct aatttataaa
      301 ttttagtcag aagtctatag cactaaaact tatttccgaa tcttgaattt caataaataa
      361 cagacatgtc ccatgaggga gctagctccc gaatcacatt agggtgctcg cacccccgaa
      421 aaaaatagta ttaattagct ttctgaaaac gataatcctc gtatttcaaa atcgtaattt
      481 ttgcattttc gatgtgttca attacacctc ctgcattttt tacagcctta aactccaacg
      541 gatacatcat atatctcaac gttggattta ctttgccacc tgaaagagac aggtcaggac
      601 cgtaagtatt taacatggtt ccaaaataca tcataacatc atatcctcta tacgcataat
      661 cagaaggcct ggtagaatac cgcgcttcaa aagcagcatt aaattctttc acctgaggca
      721 atttttggtc tacataatat ccggtcggat aatgaaaatg caaactctca aaataatcta
      781 aggaaaccga ttccagcgat aaaatatttt gtaatccgat taaggtaata tcattattgc
      841 gaccaatttt agacaggtcg cgaattaatc cgttaatata caattcatcg ttgccggcaa
      901 taaaaacaaa attacctcca ccattaatat tggatgaaat accagcaaaa ttatttacct
      961 ctttaagcgt tgctgcactg ctgccttttt gcgcattaaa tgccgcttta aattcttcgg
     1021 caattttcat ttcagcaggt ttatcattgc gataaacaac tataaaattg gcagcaacat
     1081 tattatccaa aacataatcc accattgacg acaactgtgt agttatagca ggatttgcca
     1141 tcacaaaata tttattggtg tccgcaaatg tattgctggg agataatgga gaaactaaat
     1201 aaacttcctg tttttttgca atgggtgccg ctgcttttaa actttcatta aacacagggc
     1261 ctataatgac atccgatttt tttatttctt ctttttgcag caacattgct gtgcttagac
     1321 tgtcttttaa atgattaaat acctgcacat ttaattttat acctaagcgt tctaaggtat
     1381 ccaatgcaat taatgcacct tcataaaatt cgagagaagc taatggttga taaccggtga
     1441 ttttatcttc acccattaat tttaataatt ctgctttatc agttgaaaaa ggcaatacaa
     1501 atgaaattgt atagcggtct tttttagccc acgattttaa cgggacagaa gtggcaacac
     1561 tgtcttttgc tttttcatta cctgtttttg tttctgttgt tgtagtattt tggtcaacag
     1621 gttcagtttt aacttcgata gggggtatag gtgtagtgcg cggtggataa attaccgaac
     1681 acgccgaaag gaacatcagc ccaataataa ttaccatatg tattttattc ccactcaatt
     1741 gttgccggag gttttgaact tatatcgtaa acaactcgat taataccttt aacatgattg
     1801 atgatatcag acgaaatctg tgccaataat tcgtgtggca aatgactcca gtccgcagtc
     1861 ataccatcac tggaatttac gcagcgcaat gcgataacat tttcatacgt acgttcatca
     1921 cccatcacac caacggtttg aaccggcaat aaaattgcac cagcctgcca aactttgtcg
     1981 taccaacccg attgttttaa atgattgata aaaatagcat ctgcttcctg tgtaatgcgc
     2041 actttttctg ctgtaatatc actaataatc cgaatggcta aaccgggacc cggaaatgga
     2101 tgacgatgca ggatttcatg tttcagttgt agtgtatcac ctacgcgacg aacttcatct
     2161 ttaaataaat aacgcaaagg ttcaattact ttcaaatgga gtttttcagg taaacctcca
     2221 acattgtgat gcgatttaat agtagctgaa gggccatgta ctgatacaga ttcaatcaca
     2281 tcaggataga tcgttccttg tgctaaccat tttacacctt ccagtttttt tgattctctt
     2341 tcaaaaacat cgataaatac tttgccaata attttgcgtt ttttctccgg atcactcaca
     2401 cctgccaatg ctgttataaa ttcatcagca acatcaacac caaccacatt taaacccatg
     2461 tctttataac tatgtaacac ggtgtcaaat tcgtttttac gcaataaacc gttgttgaca
     2521 aatatgctga taaggttact tcctatagct ttattcaata aaatagctgc aacgctggaa
     2581 tcaacacctc ccgataagcc taatacaact ttatcctgtt gaatagtatt ttgtaaatgc
     2641 gtgatggttt cttcaacaaa cgattccggc gtccaggttt gtttgcagcc gcaaatctgc
     2701 acaacaaaat tctcgagcat tgttttgcct tgctcagaat gggttacttc cggatgaaac
     2761 tgaatacaaa aaaccggata ttcaaatcgg cccgggttag aagcataagc ggcaacatca
     2821 atactggctg ttttggcggt gatatgaaat ccctccggta attgcgttac ggtatcacta
     2881 tgactcatcc aaacctggct ttcatcatct acaccataaa acaaattatt attacgctcc
     2941 aaaatatgca ggtaagcacg gccgtattca cgcttggaag acggttgcac tt
//
LOCUS       DAORKW010000465         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1884_length_2990_cov_6.032027, whole genome shotgun sequence.
ACCESSION   DAORKW010000465 DAORKW010000000
VERSION     DAORKW010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1884_length_2990_cov_6.032027"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="PLB46_14190"
     CDS             <1..1294
                     /locus_tag="PLB46_14190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF77722.1"
                     /translation="SSQNIIGILAITRDITETTEMNVRLEKINADLAKINEELNNTTA
                     TLIKSEERYNRMIKEVKDYAILFLSKEGLVENWNDGAARIKGYQADEIIGAHFSKFYT
                     EEDRKNDLPQNLLLTAIKNGKAIHEGWRIRKDGSKFWGNTVITAIHDNDNNIIGFSKV
                     TRDLSDKKNAEDIILAAYRELEYKNRELQKSNKELESFNYISSHDLQEPLRQIQIFAS
                     RFNDTELQNLSETGRVYFTKIYHAAKRMQNLISDLLTYSKAKTEERKYKSIDLTTLIN
                     EIKEEFAESIAEKKTVFITENLGTIHVIPFQFRQLLHNLIGNAIKFAKPDMPSIIKIH
                     METVSADAVPDINVNNELQYYHITVSDNGIGFDQQYHKRIFEVFQRLHDHQKIAGTGM
                     GLAIAKTIVENHNGIILASGEPGNGATFDIYLPVKTNN"
     gene            1304..1858
                     /locus_tag="PLB46_14195"
     CDS             1304..1858
                     /locus_tag="PLB46_14195"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRF77723.1"
                     /translation="MVLYINYNVNKICKTLLQEQLDKMELKYVLLSLGEVQLKEPISE
                     TALQQLNSNLNQCNIEIVESQKSILVQRIKDAIIEMVFTDEKMPIKFSAYLTDKLNYN
                     YNYLANLFSSETFTSIEKFILLQKTERAKQLLAQNKLNITEIAWKLNYSSTAHFSNQF
                     KSITGITPTVFQKIILKKREVNND"
     gene            1908..2354
                     /locus_tag="PLB46_14200"
     CDS             1908..2354
                     /locus_tag="PLB46_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF77724.1"
                     /translation="MVNEHLNIVFADDEENDRLLFLDALKELKIKTNVHTVNDGIELM
                     NYLNNSNNRLPHLLFLDLNMPKKNGMECLKEIRTNEHLNDIAIAIFSTSLSEKDIEET
                     LINGANVYINKPNSFDALIQVLNKVVMTAYTYQNTFFNKAHFLLRL"
     gene            2642..>2990
                     /locus_tag="PLB46_14205"
     CDS             2642..>2990
                     /locus_tag="PLB46_14205"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRF77725.1"
                     /translation="MYHFSYFKEKDRQTILAFLEQHPFAFMTGSFNNGEQVVTQVPML
                     TVERDGELYIQGHIMRNTDHHKAFMENPNVLVVFTGPSTYVSASWYSNSQMGSTWNYM
                     SVHMHGNIRWMSSV"
BASE COUNT         1113 a          492 c          527 g          858 t
ORIGIN      
        1 tagtagtcaa aatattatag gcattttggc aattaccaga gacataacgg aaaccactga
       61 aatgaatgta cggcttgaaa aaattaatgc cgacctggct aaaataaatg aggagttaaa
      121 taataccact gctaccctga ttaaaagtga agagcggtat aatcgtatga ttaaggaggt
      181 aaaagactat gctatattat ttttaagtaa agaagggctt gtagaaaact ggaatgacgg
      241 agcagctaga ataaaagggt atcaggcgga tgaaattata ggtgcccact tttcaaaatt
      301 ttatactgaa gaagacagaa aaaatgattt acctcaaaat ttactactga ccgctataaa
      361 aaatgggaaa gccatccatg aaggttggcg cattcgcaaa gatggcagta aattttgggg
      421 caataccgta attactgcca ttcatgataa tgataataat atcataggtt tttcaaaagt
      481 tacgcgcgat ttatctgaca aaaaaaatgc agaggatatt attctcgccg catatcgaga
      541 acttgaatat aaaaatcggg aattacaaaa aagcaataaa gaacttgaat cttttaatta
      601 tatctccagt cacgatttac aggaacctct gcgacaaata caaatatttg catctaggtt
      661 taacgacaca gaactacaaa atttatctga aacaggcagg gtatatttta ctaaaatata
      721 tcatgcagca aaacggatgc agaatttaat ttctgacctt ttaacctact caaaagctaa
      781 aacggaagag cgaaaatata aatccataga tctaacgacg ctgattaatg agattaaaga
      841 ggaatttgct gaatccatag ctgagaaaaa aaccgttttt ataacagaaa acctgggcac
      901 aattcatgta attccatttc agtttcgaca acttctgcac aacttgattg gaaatgctat
      961 caagtttgcc aaaccggata tgccttctat aattaaaatt cacatggaaa cggttagtgc
     1021 cgatgcggtg cctgatatta atgtaaacaa tgaattacaa tattatcaca ttaccgtaag
     1081 cgacaacggt attggatttg atcaacaata ccacaaacgc atttttgaag tattccaacg
     1141 tcttcacgac caccaaaaaa ttgcaggaac aggtatggga cttgcaatag caaaaacaat
     1201 agtggaaaat cacaacggca ttattctagc cagcggagaa cctggtaatg gagctacttt
     1261 cgatatttat ttaccagtaa aaacgaacaa ctaaattatt aatatggtac tctatataaa
     1321 ttacaacgtt aataaaattt gcaaaaccct actgcaggaa caattggata agatggagct
     1381 aaagtatgtc ctactcagtt taggagaagt acagttaaaa gaacccattt ctgaaacagc
     1441 gctgcaacag ctgaattcta acctcaacca atgtaatata gaaattgtcg aaagtcaaaa
     1501 gagtatttta gtacaacgca taaaagatgc tattattgaa atggtattta ctgatgagaa
     1561 aatgccaata aaattttctg cgtaccttac tgataaactt aactacaatt acaattactt
     1621 ggcgaatttg ttttccagtg aaaccttcac ttcaatagaa aaatttattc tattacaaaa
     1681 aacagaaaga gcaaagcagt tgttggcaca aaataaatta aacattaccg aaatcgcctg
     1741 gaaactgaat tatagcagca ctgcacattt tagtaaccag tttaaaagta taacaggaat
     1801 aacacctacc gtgtttcaaa aaattatttt gaaaaaacgg gaagtgaaca atgattaaat
     1861 agtttaaata aatcaattaa ttaacaacaa agtaaacatg gtatattatg gtaaatgaac
     1921 atctaaatat tgtatttgca gatgacgagg agaatgatcg tctcctcttt ctggatgcat
     1981 taaaagaact gaaaataaaa acaaatgtgc atacagtaaa tgatggtata gaattaatga
     2041 attacctgaa taacagcaac aataggttac cacatttgtt atttctagat ttaaatatgc
     2101 caaagaaaaa cggcatggaa tgtttgaagg aaatccgcac aaatgagcac ctgaacgaca
     2161 ttgctattgc cattttctca acttcattat cggaaaagga tattgaagaa acattgataa
     2221 atggcgcgaa tgtttacatc aataaaccta atagttttga tgctttgatt caggtattga
     2281 ataaggtggt gatgactgcc tatacttacc aaaacacgtt ttttaataaa gctcatttct
     2341 tattgcggct ttaattctca ttttggtgcc taaattccgg gcaaaaccac ataacaaatt
     2401 gggcttacga gcaacaagta atatccctga aatttgttct aatgtaatca tccataaatc
     2461 gtgagttgat tttatggttt gcaaatgcta aacaggatta aaggtttaac acttttactc
     2521 cttttttgcc tcaaactttt gcgcgttata cacagcatgg tcatagtaat ataccgattg
     2581 gtatattttg gaatttgata atatattaaa ttggttaatt ttgaccttaa acatcaccaa
     2641 catgtatcac ttctcttatt tcaaagaaaa agacaggcaa acaatactgg catttttaga
     2701 acaacatccc tttgcattta tgacaggcag ttttaataat ggcgaacagg tagtaacgca
     2761 agtgcccatg ctaactgttg agcgtgatgg tgaactatat attcaggggc atattatgcg
     2821 caataccgat catcacaaag catttatgga aaatccaaat gttttggtgg tgtttacagg
     2881 tccgagtaca tatgtgagcg catcatggta tagcaattcc caaatgggga gtacctggaa
     2941 ttatatgagt gtgcacatgc atggcaacat tcgttggatg tcatctgtag
//
LOCUS       DAORKW010000466         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1885_length_2990_cov_5.297785, whole genome shotgun sequence.
ACCESSION   DAORKW010000466 DAORKW010000000
VERSION     DAORKW010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1885_length_2990_cov_5.297785"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PLB46_14210"
     CDS             complement(<1..311)
                     /locus_tag="PLB46_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77726.1"
                     /translation="MKKVNALIIIVLMVFGFTNTVQACDNSSFSLLNLTDLGGGQYEF
                     TVEFCAGGGIGGAENGTGTWGINAVGATSFDVYPSTLTSPQTGAVYGADASFYYGSSY
                     L"
     gene            complement(562..1815)
                     /locus_tag="PLB46_14215"
     CDS             complement(562..1815)
                     /locus_tag="PLB46_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF77727.1"
                     /translation="MTRPNTHTSRGILNLTVLVAALGYFVDIYDLQLFNLVSKPSLKG
                     IGITDPALIDFWDVRLFNVQMIGMLFGGILWGIMGDLKGRKSILFGSILLYSIANILN
                     GFVDNTTQYQVVRFFAGLGLAGELGAAITLVAEIMSKENRGYGTMLIVSVGALGAVAA
                     FYITSHVDWRTSYYIGGGLGLALLVLRFGTFESTMFDNVKHENVTRGNFISLFTDRDR
                     FLKYLYCILIGVPVWYCIGVLMKFSERFAGEWGIDTSGDNGLQIRRTAIMLSYVGLCV
                     GDILSGYLSQVFRSRKKVVLGYLIMTIILAVLYLNIKSTSQTLFNIMCFLLGCATGYW
                     ALFVTIASEQFGTNIRATATTTVPNFVRGFVVPLTLGFSALSTNTGNITAALIVGAIS
                     LTLAIVSIIAVKETFGKDLDYVEMS"
     gene            complement(1835..2332)
                     /locus_tag="PLB46_14220"
     CDS             complement(1835..2332)
                     /locus_tag="PLB46_14220"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRF77728.1"
                     /translation="MPIFSIIVAVAENNIIGLKGDMPWLKLRSDLKYFKQKTTGHWCI
                     LGRKTYNALGNKVLPGRKFIIVTRDTDFVAEDSLVVHSLDAAKNHVVVEDEAEVFVLG
                     GGDIYKQSMHFVNRIYLTRIHATFEGDTQFPELGPEWNLISVDKRKMDEVNPYDHDFL
                     IFEKK"
     gene            complement(2358..>2990)
                     /locus_tag="PLB46_14225"
     CDS             complement(2358..>2990)
                     /locus_tag="PLB46_14225"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRF77729.1"
                     /translation="IIHELLWFLQGDTNIRYLKQHEVRIWDEWADEDGNLGPVYGHQW
                     RSWPTADGRTIDQITNLINQIKKNPDSRRLIVSAWNVGDIEKMALPPCHCLFQFYVAD
                     GKLSCQLYQRSADTFLGVPFNIASYALLTMMVAQVCDLQLGEFVHSFGDVHLYNDHIE
                     QARLQLTREPRALPQMKINPDVKDIFGFTIDDFELVNYDPHPHIKAKVSV"
BASE COUNT          990 a          618 c          490 g          892 t
ORIGIN      
        1 attaaataag atgaaccata ataaaatgaa gcatcagctc cataaacagc tcctgtttgt
       61 gggcttgtca aagtgcttgg gtaaacatca aatgatgtag cgcctacagc attaatgccc
      121 caggtgccag tgccattttc agcacctcca atacctcctc ctgcgcaaaa ttcaacagta
      181 aattcatact gcccaccgcc aagatcggtt aggtttaata aactaaatga tgagttgtca
      241 catgcttgaa ccgtattcgt aaatccgaat accatcaaaa caataataat aagtgcgtta
      301 acttttttca taacagacat tttttgattt tggaattata aaatttctaa cccaaaagta
      361 gatggtctgt ataaaggaat ttcgccagat gaataagtga gggaggcggg caaataagcg
      421 taaaatagag gcttcagtat ttcatgactg cttttgtaat agagtttatc tcaaattgta
      481 atacgttttc cccgaaaaca catagatgga tagtcgaaaa ctcgcatcct tattgactta
      541 caggcgttta atccagtttg tttaactcat ttcaacataa tccaaatctt tgccaaaagt
      601 ttctttaaca gcaataattg atacaattgc caatgtaagt gatatcgctc cgacaataag
      661 tgcagcggta atattaccgg tattagtgct taaggcagaa aaacctagtg taagcgggac
      721 aacaaatccc ctgacaaaat tagggacagt agtagtagcc gttgctctga tatttgttcc
      781 aaactgttct gaagcaattg taacaaacaa agcccaatat cccgttgcac atccgagtaa
      841 gaaacacatg atattaaata aagtctgaga agtacttttt atgttgagat ataataccgc
      901 taatatgatg gtcataatta aatagccgag cactactttt tttctggagc gaaatacctg
      961 actcaaatat ccgcttaata tatcgccaac acataatccg acataactca acataattgc
     1021 tgttcggcga atttgcagac cattatctcc gctggtatct attccccatt cgcctgcaaa
     1081 acgttcacta aatttcatta atacgccaat gcaataccaa acaggaacac caattaaaat
     1141 gcaatataaa tatttaagaa atcggtcgcg gtcggtaaat aatgaaataa aatttcctct
     1201 ggttacattt tcgtgtttca cattatcgaa catggtactt tcaaatgtgc caaatcgcaa
     1261 caccaataat gctaatccta aacctcctcc aatataatag gatgtccgcc aatcgacatg
     1321 agaagtaata taaaaagcag ccactgctcc taatgcacca acacttacaa tcaacatagt
     1381 gccatatccg cgattttctt tgctcataat ttctgcaacc aacgtaattg cagcgcctaa
     1441 ttctccagct aatcccaaac cggcaaaaaa tcgtacaacc tgatattgag tggtattatc
     1501 tacaaaacca tttaaaatat tggctataga atataataaa atacttccaa ataaaatcga
     1561 ttttcggcct tttaaatccc ccattattcc ccacaaaatt ccgccgaata acatgcctat
     1621 catttgcaca ttaaataatc tcacatccca aaaatcaatt agtgctgggt cggtaatgcc
     1681 aattcctttt aaactgggtt tgctgactaa attaaataat tgcaagtcgt aaatatctac
     1741 gaagtagcct agcgctgcca ctaaaacggt aagattgagt attcccctgg aggtgtgtgt
     1801 gttaggtcgt gtcataatca ggggatgaaa ttatctattt tttctcaaaa atgagaaagt
     1861 cgtggtcgta aggatttact tcatccatct tccttttatc tactgatatc agattccatt
     1921 ccggtcccaa ttcagggaat tgtgtgtcgc cttcaaatgt ggcatgaata cgtgtaagat
     1981 atattcgatt tacgaaatgc atagattgtt tgtaaatatc acctccaccc aaaacaaata
     2041 cttcggcttc atcttcaacc actacatgat ttttggcagc atctagtgaa tgcaccacca
     2101 aactatcttc agcaacaaaa tcagtgtcgc gtgttacaat gataaatttt cttccgggta
     2161 aaactttatt gcccaatgca ttatacgttt tacgtcctaa aatacaccag tgacctgttg
     2221 ttttttgttt aaaatatttt aaatcagacc ttaatttaag ccagggcata tcacctttta
     2281 atccaatgat attattttct gcaacggcaa ctatgatgga aaaaataggc atgttttgta
     2341 tttaaaaaag aaaaaaatta aacacttact ttggctttaa tatgcggatg agggtcgtaa
     2401 ttaactaact caaaatcatc gatggtaaat ccaaaaatat ctttcacatc gggatttatt
     2461 ttcatttgtg gcaaagcacg tggttcgcgc gttaattgta aacgagcctg ttcaatatgg
     2521 tcgttataaa gatgtacatc gccgaatgaa tgtacaaatt cacctaactg caaatcacaa
     2581 acttgcgcaa ccatcatggt taataatgca taactggcaa tgttaaatgg aacgcctaaa
     2641 aaagtatctg cactgcgttg atacaattgg caacttaatt taccgtcagc cacataaaat
     2701 tggaacaaac aatggcaagg aggtaatgcc attttttcga tatcgccaac gttccaggcg
     2761 ctgacaatta aacgtctgga atccggattt tttttaatct ggttaattaa attggtgatt
     2821 tggtcgattg ttctgccatc agcagttggc cagcttctcc actggtgacc atataccgga
     2881 cctaaattac catcttcatc agcccattcg tcccaaatac gcacttcatg ttgcttgaga
     2941 taacgaatat tggtatctcc ctgcaaaaac cacaataatt cgtgaatgat
//
LOCUS       DAORKW010000467         2986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1888_length_2986_cov_4.443193, whole genome shotgun sequence.
ACCESSION   DAORKW010000467 DAORKW010000000
VERSION     DAORKW010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1888_length_2986_cov_4.443193"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLB46_14230"
     CDS             <1..769
                     /locus_tag="PLB46_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77730.1"
                     /translation="ETIIFDRTGLYGAEKGFELNYLNPVIFYRAVESGLGSSDNVVLG
                     TNWKWNFLQRFSLYGQLVLDELVVGELLANDGWWGNKYAIQSGLKYINAFGINQFDLQ
                     YEYNMARPYTYSYEDENGSSYTHYAQPLAHPLGANFSEHLVALWYQPFPKIVINNRLL
                     MANYGTDPAGENYGGNVFLDYNTYSNEYGNFTGQGINNQLLMNDLVVSWQFWHNASLD
                     FRCIYRDLQVSASGQNANELFFSVGVRLNEVLKQYLY"
     gene            complement(770..1312)
                     /locus_tag="PLB46_14235"
     CDS             complement(770..1312)
                     /locus_tag="PLB46_14235"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRF77731.1"
                     /translation="MEFNYLITERLKLRIITPAVLQYVYHNYNDEQLITFLGLQNATQ
                     LAVEKSKFEKGYTTYKIDYLNFQLLDKTNLNVIGWCGFHSWYPEHQRAEIGYHIIEEI
                     NKGKGYMSEAFPAIIDYGFQHMQLNRIEALIGPQNIASQQLVNKLGFVKEGVMRQHYF
                     KNEQLEDSVIYALLKADYYK"
     gene            complement(1333..2160)
                     /locus_tag="PLB46_14240"
     CDS             complement(1333..2160)
                     /locus_tag="PLB46_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77732.1"
                     /translation="MKLYISFFVLLTMAGLGLANGQNVHYLEKLDYKSRWYIYWGWNL
                     DGYSDSDIHFKGDDYDFTLQNVAANDRQTPFEARTYCNPLRATIPQYNFRVGYFLNSH
                     YNISFGIDHMKYVMQQKQIVRIDGYINNSETQYDGVYDNNEILLAADFLRFEHTDGLN
                     YVNTELRRMDNLFAIGPVKVNAVEGISAGFLWPKTNCTLLNNERYDEFHIAGFGTGLV
                     AGINLELFQYFFLQSELKGGYINMPSIRTTAFESDIADQQFFFAQWNVVFGVNFNLH"
     gene            complement(2229..>2986)
                     /locus_tag="PLB46_14245"
     CDS             complement(2229..>2986)
                     /locus_tag="PLB46_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77733.1"
                     /translation="GRIGQNADIQIFLKLENYSSENSEIYSVSGYYLYTKINTPIPIV
                     GIYDGTLTLYVFNNSERGDSLVYFNYELSHFETISYCKEISGFTEKITFDGNTGTWTD
                     GKKSLPVTLNDSDLRVIHQHEYLIITTEDQQNMLDLQEISNPDKNFELINACSNDQQI
                     QILLRFSYPSNPYVNGRCGAGFESGYMELQFDKNTYWVTHQRVLFESCYEDVFIESAN
                     ETEENVYTTVIFNGAGEQKTITLNTTDCRIDIK"
BASE COUNT          933 a          530 c          524 g          999 t
ORIGIN      
        1 cgaaacaatt attttcgacc ggacaggatt atatggtgct gaaaaaggtt ttgaattgaa
       61 ttatttaaat ccggttatat tttatcgcgc cgtggaaagt ggtttgggga gtagcgacaa
      121 tgtggtatta ggaaccaatt ggaaatggaa tttcttacaa cggttctcct tgtatggtca
      181 attggttttg gatgaattag tggttgggga attattagcg aatgatggtt ggtggggaaa
      241 taaatatgcg atacaatccg gattaaaata tatcaacgca tttggtatta atcaatttga
      301 cttgcaatat gaatacaata tggcaagacc ttacacctat agttacgagg acgaaaatgg
      361 ttccagttat acgcattatg cgcaaccact ggcacatccg ctgggagcca atttttcaga
      421 acatcttgta gctttgtggt atcaaccttt tccaaaaatt gtcatcaata ataggttgtt
      481 aatggccaat tacggcaccg atcctgccgg agaaaattat ggtggaaatg ttttcctcga
      541 ttataatacc tattccaacg aatatgggaa ttttaccggt cagggaatta acaatcaact
      601 actaatgaac gaccttgtag tttcctggca attttggcat aatgccagtt tagatttcag
      661 atgtatttac cgcgatttac aagtaagtgc ttcgggacaa aatgctaacg aattattttt
      721 tagtgttggg gtgagattaa atgaagtgct taaacagtat ttgtattaat cacttgtaat
      781 aatcagcttt taataatgca taaataacgg aatcttccaa ttgttcgttt ttaaaataat
      841 gttgtcgcat tacaccttct tttacaaatc ctaacttgtt taccaattgc tgagaagcga
      901 tattttgagg accaattaat gcttcaattc tatttaattg catgtgttga aatccgtagt
      961 caataatagc cggaaaagcc tcggacatat aacctttgcc tttatttatt tcctcaataa
     1021 tatgataacc gatttcagcg cgttgatgtt caggatacca ggaatgaaat ccacaccaac
     1081 caatcacatt caaattggtt ttgtccagta attgaaagtt gagataatct attttataag
     1141 tggtgtatcc cttttcaaat ttcgattttt caacggctaa ttgtgttgca ttctgcaatc
     1201 ctaaaaaagt aattaattgt tcatcgttgt aattgtgata aacatattgt aaaactgccg
     1261 gcgtaattat ccgcaatttt aatcgttcgg ttatcaggta attaaattcc atggtggaaa
     1321 agatacaaat gattagtgga gattaaaatt aacaccaaac actacattcc actgtgcgaa
     1381 aaagaattgt tggtctgcaa tatccgattc aaatgccgtt gtccgaatgc ttggcatatt
     1441 aatgtatcca ccttttaact cactctgcag gaaaaaatat tggaagagtt ccaaattaat
     1501 tccagctacc aaacccgttc caaatcctgc aatatgaaat tcatcgtaac gctcattatt
     1561 caataaggta caatttgttt tcggccataa aaaaccggca cttattcctt ctacagcatt
     1621 tacttttacc ggacctattg caaaaaggtt atccattctg cgcaattccg tatttacata
     1681 attcaatccg tcggtatgtt caaaacggag gaaatctgcc gccaataaaa tttcattatt
     1741 atcatataca ccgtcgtact gagtttcaga gttgttgatg taaccatcta tacgcacaat
     1801 ttgtttttgt tgcatcacat atttcatgtg gtcaatacca aatgatatat tgtaatgact
     1861 atttaaaaaa taaccaaccc ggaaattata ttgcggtata gtagctcgta aaggattaca
     1921 ataagttctg gcttcaaaag gtgtttgcct gtcgttagca gctacatttt gcagcgtaaa
     1981 atcataatca tcacctttaa aatgtatatc agaatccgaa tatccatcga ggttccatcc
     2041 ccaatagata taccatctgc ttttatagtc caatttttcg aggtaatgta cattttgacc
     2101 attagccaat cccagacctg ccatagtaag caatacaaaa aacgatatgt ataatttcat
     2161 tgaaaaataa tgttaaccct tacaattgcg ggacaaaggt ataataaatg taaaaatcag
     2221 gctgttattt atttgatgtc aatcctgcaa tctgttgtat tgagggtaat ggttttttgc
     2281 tctcctgccc cgttaaagat tacagtagta tatacattct cttcggtttc gttagcgctt
     2341 tcaataaaca catcttcata acaactttca aataatactc tttggtgagt aacccaatag
     2401 gtatttttat cgaattgtaa ttccatatag ccggattcga aacctgcccc acatctgcca
     2461 tttacataag ggttagacgg ataggaaaac ctcaataata tttgtatttg ctggtcatta
     2521 ctgcaggcat ttattagttc aaaatttttg tccggatttg agatttcctg caagtccagc
     2581 atattttgtt gatcttctgt cgttataatc aaatattcgt gttggtgaat tacgcgcagg
     2641 tcactatcgt ttaaggtaac aggcaatgac tttttaccat ctgtccaggt accggtattg
     2701 ccatcaaacg taattttttc ggtaaatcct gaaatttcct tgcagtagga gatagtttca
     2761 aaatggctta attcgtaatt aaaataaaca aggctatctc ccctttcact attgttaaaa
     2821 acatacaggg ttaatgttcc atcgtaaatg ccaactatcg gtataggggt atttattttt
     2881 gtatatagat agtatccgct aacagaataa atttcggaat tttcagatga atagttttcg
     2941 agtttcaaga aaatctgaat gtcggcattt tgtccaattc gcccat
//
LOCUS       DAORKW010000468         2982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1890_length_2982_cov_3.538435, whole genome shotgun sequence.
ACCESSION   DAORKW010000468 DAORKW010000000
VERSION     DAORKW010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1890_length_2982_cov_3.538435"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /locus_tag="PLB46_14250"
     CDS             <1..1787
                     /locus_tag="PLB46_14250"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type H(+)-translocating pyrophosphatase"
                     /protein_id="HRF77734.1"
                     /translation="MYGFEGQAAAVKVINVVTGFSFGASSIALFARVGGGIYTKAADV
                     GADLVGKVEAGIPEDHPLNPATIADNVGDNVGDVAGMGADLFESYVGAIVGTMVLGTA
                     FMAGSIAEGGSFMDDGFNGLSAVLLPLVLGGVGILTSIIGTFFVRVKEGGNPQKALNG
                     GAIGSSIVMVILSYFIITWMLPSSFSYVDVLYGGKERTITSLGIFIATIVGLIAGTAI
                     GFITEYYTGTGKKPVMKIVRQSSTGAATNIIAGLSVGMMSTAWPVLILGASIMISFAC
                     GGLYGIAIAAVGMLANTGIQLAVDAYGPISDNAGGIAQMADLPKEVRHRTDKLDAVGN
                     TTAAIGKGFAIGSAALTALALFGAFMQVTGLTKIDISQAHVITGLFVGAMLPFLFSAM
                     AMQSVGKAAMAMIEEVRRQFASIPALKAALEVMKRNGEKEMNEWSQADQDTFNAAEGK
                     AEYAKCVEISTKAAVKEMVLPGLMAVLVPVIVGFAPKVLGLNTGENVLMGPEMLGGLL
                     AGVTVSGVLMAIFQANAGGAWDNAKKMFEEGVEINGQTYYKGSEPHKAAVVGDTVGDP
                     FKDTSGPSINILIKLMSVVSLVLASLMI"
     gene            1932..>2982
                     /locus_tag="PLB46_14255"
     CDS             1932..>2982
                     /locus_tag="PLB46_14255"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77735.1"
                     /translation="MKHIVTAFFIVCLTTQQVKAQTDTVELNYIAEYYFENLLPYSAY
                     TSCFHQQGEPFIYAACRDLGVVTFDITDVANPVPIDTVTLFAFNGFKPTNLSQHGNYL
                     YVATGGFDGFAQNAGLAILDIADPTHPVIRDLWDSIAFNQGAAIVITDGEYAYLGGME
                     EGVIILDVTEKTHIKYVSSILPDPNFPEVPGLFSVPNARGLAFYEEDKLLVANDAGGL
                     RVIDISNKLAPVEIEKYVNTDIEDIAQSAYNNIVVVDHYAYITVDMCGMEVVDLNTNP
                     ISNVMWFNPWDCDSTNWIGRPGHTNEIKSVGDSLLFVSGGDSELLIFDITERINPRLV
                     GSYAHILDSIATWAID"
BASE COUNT          855 a          452 c          679 g          996 t
ORIGIN      
        1 atatgtatgg tttcgaagga caggctgctg ccgttaaagt aattaacgtt gttaccggct
       61 tttcttttgg cgcatcttca attgcattat ttgctcgcgt tggcggtgga atttatacca
      121 aagctgccga tgttggtgcc gatttagtag gtaaagttga ggccggaatt ccggaagatc
      181 atcctttaaa ccctgcaaca attgccgata acgttggaga caatgttggt gatgttgccg
      241 gtatgggtgc cgatttattt gaaagttatg taggtgctat tgttggtaca atggtattag
      301 gaacagcttt catggcgggc tctatagctg aaggcggtag ttttatggat gatggattta
      361 atggcttgtc tgctgtttta ttacctcttg ttttaggtgg tgtgggtatt cttacttcta
      421 ttataggaac attttttgtt cgtgtaaaag aaggtggtaa tcctcaaaaa gcattgaacg
      481 gtggtgcaat tggctcatca atagtaatgg tgattttatc ttatttcata attacctgga
      541 tgttgccttc atcatttagt tacgttgatg tgttgtatgg tggtaaagaa cgtactatta
      601 catcattagg tatttttatt gctactattg ttggtttgat tgccggaact gctatcgggt
      661 ttataactga atattatacc ggaacaggta aaaaaccagt gatgaaaatt gtgcgtcaaa
      721 gttcaacagg tgcagctact aatattattg ccggcttaag tgttggtatg atgtcaacag
      781 catggcctgt gcttatttta ggtgcatcta tcatgatttc atttgcttgt ggtggtttat
      841 atggtattgc gattgccgca gttggaatgt tagcaaacac cggtattcaa ttagcagttg
      901 atgcttacgg cccaatttcg gataatgcag gtggaattgc tcaaatggct gatcttccga
      961 aagaagttcg tcaccgcacc gataaattag atgcagttgg aaatactact gctgctattg
     1021 gaaaaggatt tgcaattggt tcagctgctt taactgcatt agcattgttt ggtgcattta
     1081 tgcaagtgac cggtttaact aaaatcgata tttcacaagc tcacgttatt acaggtttat
     1141 ttgttggcgc catgttgccg tttttatttt cagcaatggc aatgcaaagt gttggtaaag
     1201 cagcaatggc aatgattgaa gaagtgcgga gacaatttgc ttcaattcct gcattaaaag
     1261 ctgcattgga agtaatgaaa cgcaatggag aaaaagaaat gaacgaatgg tcacaagcag
     1321 accaggatac atttaatgct gcagaaggta aagctgaata tgcaaaatgt gtggaaattt
     1381 caacaaaagc tgctgtaaaa gaaatggtat tacctggttt aatggctgtt ttagttccgg
     1441 ttattgttgg ttttgcccct aaagttttag gattaaatac aggtgaaaat gtactaatgg
     1501 gacctgaaat gttaggtggt ttgttagccg gtgtaaccgt gagtggtgtt ttgatggcaa
     1561 tttttcaagc taatgccggt ggtgcatggg acaatgctaa aaaaatgttt gaagaaggtg
     1621 tagaaattaa cggtcaaacc tattacaagg gcagcgaacc gcataaagct gcagtggtag
     1681 gagatacagt tggtgatcca tttaaagata cttcaggacc ttcaattaat atcttaataa
     1741 aactgatgag tgttgtaagt cttgtgcttg cttcattaat gatttaattt aaacttaaat
     1801 aaagtaaaga ccccggaagt atattccggg gtcttttcat gtacgtattt cttgccagtt
     1861 gtagaggcca tttaagcact taaacatacg gtaaatgcca ttttagcatg aaatttggta
     1921 gtttagtagg catgaagcac attgttacag ccttttttat cgtgtgtctt accacgcaac
     1981 aggtaaaagc acaaaccgat accgttgaac tcaactacat tgctgaatat tattttgaaa
     2041 atttattgcc ctactcagca tatacttcct gttttcatca acagggcgaa ccttttattt
     2101 atgcagcatg tcgcgacttg ggtgttgtta cttttgatat aactgatgtt gctaatcctg
     2161 tgcctataga cacggtaact ttatttgcat ttaatggttt taaaccgacc aatttatcac
     2221 aacatggtaa ttatttatat gtggcaacag gtggttttga tggatttgca caaaatgcag
     2281 gtttagccat attagatata gctgatccaa ctcatcctgt aattcgggat ttatgggatt
     2341 caatagcatt taatcaagga gcagcaattg taattactga tggtgagtat gcctatttag
     2401 gtgggatgga agagggtgtt attattttag atgtcactga aaaaacgcat ataaaatacg
     2461 tttcatccat tttacctgac cctaattttc cggaagtgcc gggattattt tcagtaccaa
     2521 atgcgcgagg tttagccttt tacgaagagg ataaattgtt agtagcaaac gatgccggtg
     2581 gtttacgtgt aattgatatt agcaataaat tagcccctgt tgaaatagaa aaatatgtaa
     2641 ataccgatat agaggatatt gcacaatctg cttataacaa tattgttgtt gttgatcatt
     2701 atgcctatat cacagtagat atgtgtggta tggaagtagt agatttaaat accaatccaa
     2761 tttcaaatgt tatgtggttt aatccatggg attgtgattc tacaaattgg attggccggc
     2821 cagggcatac caacgaaata aaatcggtgg gagatagttt attatttgta tcgggtggtg
     2881 attcagaatt attaattttc gatattacag aaagaattaa tcctcgtttg gtgggaagtt
     2941 atgcgcatat tttagatagt attgctacct gggctataga tg
//
LOCUS       DAORKW010000469         2976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1894_length_2976_cov_4.817528, whole genome shotgun sequence.
ACCESSION   DAORKW010000469 DAORKW010000000
VERSION     DAORKW010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1894_length_2976_cov_4.817528"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PLB46_14260"
     CDS             complement(<1..807)
                     /locus_tag="PLB46_14260"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRF77736.1"
                     /translation="MLVSTECTKAANFTKQVLAPIYYFYEKNTMYNQSMLKADSLKGK
                     TIVVTGGGTGLGRSMSQYFLTLGANVVITSRKLDVIEKTAAELSAATNGNCFAVACDV
                     RIYEQVENMLQATISKFGQVDILLNNAAGNFISPTERLSHRAFDTVVDIVLKGSYNCT
                     LAFGKYWIANKIPANVLSIVTTYAWTGSGYVVPSACGKAGVLALTRSLAVEWGKYGIR
                     LNAIAPGPFPTEGAWSRLMPPDMKDDFDIAKRNAIGRVGEHQDLANLAAYL"
     gene            842..1234
                     /locus_tag="PLB46_14265"
     CDS             842..1234
                     /locus_tag="PLB46_14265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRF77737.1"
                     /translation="MEAHPIKVRGFHCDMFGHVNNARYLEFLEEARWEWLNRITTFDF
                     FEKHHMSFVVVNININYKRPSVLNDELMISVTLKNIGNKSAAVNQVITRKSDLKVVAD
                     ADVTFAMVDNNTGKAIELNDEIKKILGG"
     gene            complement(1420..2694)
                     /locus_tag="PLB46_14270"
     CDS             complement(1420..2694)
                     /locus_tag="PLB46_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF77738.1"
                     /translation="MANRKLKVSAMAENLIGSEIIKLAGEVNERMKRGEKIYNLTIGD
                     FDPKIFPIPQELEQEIINAYKNNETNYPAANGIESLRKAAQHFVGSHNGLKYDTDEFL
                     IAGGGRPLIYATYITLLDKGDKVIYPIPSWNNNHYTHLSFCEKIEVETQPENNFMPTA
                     AEIAPHIQEATLIALCSPLNPTGTAFSKSALAEICDLVVAENKRRSPDQKPLYILYDQ
                     LYWTLTFGETHHYDPVSLRPELRDYVIYIDGLSKAFSATGVRVGWSFGPKYIIDKMKS
                     ILSHIGAWAPKAEQVAAAKYLMDDAAINRFLSDYKQQIQNRLEGFYQGIQSLKNKGYH
                     VDCIAPQSAIYLTVKCDLRGAIKPDGNKINSMADVAAYLLNDAHIALVPFYAFGSDKE
                     SPWFRLSVGTCSMDDVNGSIASLENALGQLKW"
BASE COUNT          894 a          583 c          530 g          969 t
ORIGIN      
        1 tagataagca gccagattgg ccaagtcctg atgttcgcca acgcggccaa tggcattgcg
       61 tttagcgata tcaaaatcat ctttcatatc cggtggcata agacggctcc aggcaccttc
      121 tgtaggaaac ggccccggtg caatagcatt taaacgaata ccatatttgc cccattcaac
      181 cgctaaactg cgcgtaagtg ccaaaacacc tgctttgccg caagcgcttg gaacaacata
      241 accacttcct gtccatgcgt aggtagtaac aatactcaat acattggcag gaattttatt
      301 tgcaatccag tattttccaa aagctaaggt gcagttgtaa ctgcctttta acacaatatc
      361 tacaacggtg tcgaatgcac ggtgactcaa tctttcggta ggggagatga agttgccggc
      421 tgcattgttc aacaggatat ctacttgtcc gaatttggaa attgttgctt gtaacatatt
      481 ttctacctgt tcatatatcc gcacatcgca ggccactgca aaacaatttc cgtttgttgc
      541 agcgcttaat tcagcagccg ttttttctat tacatccagt tttctggagg tgattaccac
      601 attggcacct aaggttaaaa aatattggct catacttctg cccaaacccg ttcctccgcc
      661 cgttactaca attgtttttc ctttcaggct gtccgctttt aacatcgatt gattatacat
      721 ggtatttttt tcataaaagt agtaaattgg ggctaatacc tgtttggtaa aatttgctgc
      781 ttttgtacat tccgttgata ctaacatgta aaaccttttc ttcatctttg taaaaaaaag
      841 aatggaagcg catccaatta aagtaagggg atttcattgc gatatgtttg gacatgtgaa
      901 taatgcccgc tatctcgaat ttttggaaga agcccgttgg gaatggttaa acagaataac
      961 cactttcgat ttttttgaaa aacaccatat gagttttgta gtagttaata taaatattaa
     1021 ttataaacga ccttcagtgc taaacgatga attaatgatt agtgttacac tgaaaaacat
     1081 tggtaataaa agtgctgctg ttaatcaggt aatcacacga aagtccgatt taaaagtagt
     1141 agctgatgct gatgttactt ttgcaatggt agacaacaac acaggtaaag caattgaact
     1201 aaatgatgaa ataaaaaaaa tattaggggg ctaaaccggt ctacaagaaa ctgcttctga
     1261 ttatggtgta tttatggcat taaataaaat ctgcacccaa acaaatattt taataactag
     1321 tatcagggaa aattatttcc agcattagtt atacctgaaa ttgcaattaa ctataccatc
     1381 atttgttaag attaacatca taaaactgct aattatgaat taccatttta gctgacccaa
     1441 ggcattttct aaacttgcta tactgccatt aacatcatcc atactgcaag tgccaacact
     1501 taagcggaac caaggacttt ctttgtcgct gccaaatgca taaaacggca cgagtgcaat
     1561 atgcgcatcg tttaacaggt aagctgcaac atctgccata gaatttatct tattaccatc
     1621 cggttttatg gcaccgcgta aatcgcattt aaccgttaaa taaattgcag attgcggtgc
     1681 aatacagtca acatgataac ctttgttttt taatgattgt ataccttgat aaaatccttc
     1741 taaacggttt tgaatttgtt gtttgtaatc cgataaaaaa cgattgatag cagcatcatc
     1801 catgagatat tttgccgcag caacctgttc agcttttggt gcccaggctc caatatggct
     1861 taaaatactc ttcattttat caatgatgta tttaggtcca aaactccaac ctacacgaac
     1921 acctgtagca gaaaatgctt tacttaaacc atcaatataa attacataat cacgtaattc
     1981 aggtcgtaat gaaacagggt cgtaatgatg cgtttcacca aaggttaaag tccagtataa
     2041 ttggtcgtat aagatgtata acggtttttg gtcagggctt cttcttttat tttctgctac
     2101 taccaaatcg cagatttcag ctaaagcaga tttcgaaaat gcagttccag ttggatttaa
     2161 tggcgaacat aaagcaatta aagttgcctc ctgaatatgt ggtgcaattt cagccgcagt
     2221 tggcataaaa ttattttcag gttgagtttc aacttctatt ttttcgcaaa aacttaagtg
     2281 cgtgtaatga ttgttgttcc aacttggtat aggataaatt actttatccc ctttatctaa
     2341 taatgtaata taggtagcat aaattaatgg acgtccacca cctgcaatca aaaattcatc
     2401 ggtgtcgtat tttaatccgt tgtgactacc aacaaaatgt tgagccgctt ttctcagcga
     2461 ttcaatacca tttgctgcag ggtaattggt ttcattattt ttataggcat taataatttc
     2521 ctgttccagt tcttgtggaa taggaaatat ttttgggtcg aaatcgccaa tcgtaagatt
     2581 gtaaattttt tcgccgcgtt tcatacgttc gttaacttca ccggcaagtt tgataatttc
     2641 tgagcctatc agattttctg ccattgcaga aaccttgagt tttctattag ccatagtttg
     2701 tccttcaccc atagctgcaa aataatacaa tttttttaac gttttccgcc ttcagctgcc
     2761 attttatttg agggttttca ccctgttttt acggtaaaat gccaccaatc aacggttaac
     2821 cctgtggaga ttttgttaaa aacgttttgg gtgcccttca cttctattgc caagtaggct
     2881 tatacaccct gcctgaggcg gcttggagat atccgtataa attttagtaa atttgaatag
     2941 tgatatgaaa acagccttca cattttgcct attttt
//
LOCUS       DAORKW010000470         2956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1910_length_2956_cov_6.776629, whole genome shotgun sequence.
ACCESSION   DAORKW010000470 DAORKW010000000
VERSION     DAORKW010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1910_length_2956_cov_6.776629"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..2729
                     /locus_tag="PLB46_14275"
     CDS             162..2729
                     /locus_tag="PLB46_14275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF77739.1"
                     /translation="MRTLVIITTAFALLLPAIVSAQAGIYPDILWERNYGGTASDYLQ
                     DACATSDSGYLLFGQSRSIGDDVGALHGGQDYWLLKIDSVGNTVWNKTYGGADDEYGI
                     AIMQSSNNNFVISGITYSDGTGDVVNNFDDGWEPYSTDTWNVRIDNLGSIQWSENYGG
                     SKDDGPDFLYEAFGQKILTVGYSMSNSYMVDGNNGYSDFYIFLTDSNGSVEWSVTLGS
                     SSYDYAYGATQLADSNFLIVGHSGTNNLDFAGGYGASEGWLVLIDKSGEVIWKKNYGG
                     SAADDLLAVMPLNSDDLILSGYSMSSDFDLNINYGGFDFWIMRTDSIGNVQWSTSLGT
                     AGWDLVKHMEISDSTIYVCGYNGGNSGVVTDNHGSLDFWLVALDFYGNILWHRSYGGT
                     LSDVANNIIINPDKTMLLSGVSYSNNGDVGAHYGTSTYSDFWVIKLSEPCMHAAWYAD
                     EDGDSYGNVDIVEFSCTAIPGFVSDSTDCNDTDSLVNLMTTDICNNLDDNCNGIFDED
                     ALAYLWYFDGDSDSYGDATTDSVACFTPIGYTADSTDCDDTDSLVNLLAIDLCNNLDD
                     NCNGIFDEDALAYLWYFDGDSDSYGDATTDSVACFTPIGYTADSTDCDDTNNMINIGA
                     IEICNAFDDNCNTIIDEGLPTFVFFADFDADTFGNADSTIESCFGFVIGFVSDSTDCN
                     DTMFSIFPGAVEVCNYLDDDCDILIDENLIYVWQFADSDNDYFGDALTDTLACIDIPG
                     YVLDSTDCDDTNPDIYPGAIEILNGLDDDCNQISDEGLMVNNLNKVISIYPNPALDYI
                     FIQSDAVHNITIYNLTGAAIQNLLLLPGLNSFSVQNYSSGAYLVKVDNNELLIWVKE"
BASE COUNT          882 a          444 c          630 g         1000 t
ORIGIN      
        1 atgctacttt ttgggtcgct aagacctgac aggtttaaac ctgtcattta tgaattgggc
       61 atagaagcag gaagtgccgg cgtattaagc ttggatttac ttgatacaaa gcagccgctt
      121 cgagcaaaaa aagtgtatct ttaacttcat atctattcca aatgaggaca ctcgtaatta
      181 ttactaccgc atttgcactt ctactccccg ctattgttag tgcacaggcc ggaatatatc
      241 ctgatatact atgggaaagg aattacggtg gaactgcttc agattattta caggacgcct
      301 gtgccacatc tgacagtggc tatttattat ttggtcaaag taggtcaata ggtgatgatg
      361 ttggcgctct tcatggcggg caggattatt ggcttttaaa aattgacagc gtaggtaata
      421 ctgtttggaa taaaacttat ggtggtgcag acgatgaata tggcatagca atcatgcaat
      481 caagtaacaa taattttgtg atttcgggga taacctattc tgacggaacc ggcgacgttg
      541 tcaataattt tgatgatggc tgggagccat attcaactga tacatggaat gtccggatag
      601 acaatctagg ttcgatacaa tggagcgaaa attatggcgg atctaaggac gatggccctg
      661 attttttata tgaagcattt ggccaaaaaa tactaactgt tggttactct atgtctaata
      721 gttatatggt ggatggcaat aatggttatt cagatttcta tattttttta actgacagta
      781 atggctctgt tgaatggagt gtaacattag gcagcagcag ttatgattat gcctatggcg
      841 caacacaatt agctgatagt aattttttaa ttgttggtca ttcaggtaca aataatttag
      901 attttgccgg tggatatggg gcaagtgaag gctggctggt tttaatagat aaatcaggtg
      961 aagtgatttg gaaaaaaaat tatggtggtt ctgctgctga cgatctactt gctgtaatgc
     1021 ctttaaacag tgatgattta atactgagtg gttacagcat gtcatctgat ttcgacctca
     1081 atataaatta cggtggtttt gatttttgga ttatgagaac ggatagtatt ggtaatgtcc
     1141 aatggagtac aagtcttggc actgccggat gggaccttgt aaaacatatg gaaatatcag
     1201 attccaccat ttatgtttgt ggctacaatg gtggaaattc cggagtagtt accgataatc
     1261 acggcagttt ggatttttgg ttggtggcgt tagactttta tgggaatatt ctttggcatc
     1321 gatcgtacgg tggaacatta agtgatgtgg ccaacaacat tattataaat ccagacaaga
     1381 ctatgctttt aagtggagta agttactcta ataatggaga tgttggtgca cattatggca
     1441 cttcaacata ttctgatttc tgggttataa aactaagcga gccttgcatg catgcagctt
     1501 ggtatgcaga cgaggacgga gatagttatg gcaatgttga tattgttgaa ttttcatgta
     1561 ccgctattcc gggttttgta agtgatagta ccgattgcaa tgacacggat agtttagtaa
     1621 acctaatgac aacagatata tgcaacaatt tagatgataa ctgtaatggc atttttgatg
     1681 aagatgcctt ggcttattta tggtattttg atggcgatag tgattcctat ggagatgcaa
     1741 caacagattc tgtagcttgc tttacaccaa taggttatac tgcagatagt accgattgtg
     1801 acgacacaga tagcttagta aacttattgg caatagattt atgcaacaat ttagacgata
     1861 actgtaatgg catttttgat gaagatgcct tggcttattt atggtatttt gatggcgata
     1921 gtgattccta tggagatgca acaacagatt ctgttgcttg ctttacacca ataggttata
     1981 ctgcagatag caccgattgt gatgatacta ataatatgat aaacattggg gcgattgaaa
     2041 tttgcaatgc ttttgacgat aattgtaata ctataattga tgaaggctta ccaacctttg
     2101 tttttttcgc cgattttgat gcagatactt ttggcaatgc ggacagtact attgaaagtt
     2161 gttttggttt tgttattggc tttgtttctg acagcacaga ttgcaatgac acaatgtttt
     2221 caatatttcc gggtgcagtt gaagtttgca attaccttga tgatgattgc gacattttaa
     2281 ttgatgaaaa cctcatatat gtatggcaat ttgccgattc cgataatgac tattttggtg
     2341 atgcgctcac tgataccttg gcctgtattg atataccagg ttatgtatta gactctaccg
     2401 attgtgatga tactaatcct gatatttacc ccggcgctat agaaatttta aatggcttag
     2461 atgatgattg taatcaaatt agcgacgaag gattgatggt taataattta aataaagtaa
     2521 ttagtattta tccaaaccct gcacttgatt atatttttat tcaatcagat gcagttcaca
     2581 acatcacaat ttataattta accggggctg caattcaaaa ccttctgctt ttacccgggt
     2641 taaattcctt ttcggtacaa aattattcca gtggcgcgta tttggtcaag gttgataata
     2701 atgagttgtt aatttgggtg aaggagtaag tcattttttt taaattaatt taatacaatg
     2761 cgtttactct ttgctgctaa aggatacctt gtgatgatga aaattaaaaa tattcctggt
     2821 aattaaattg ccatgaaaat taaattggtg aattattaaa aaatcaaaat gacaaatttt
     2881 catacattat tttttcagca tttatctcct tcaattcata attaaaatag atgcacctta
     2941 attaaaacaa ataatt
//
LOCUS       DAORKW010000471         2956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1911_length_2956_cov_4.087211, whole genome shotgun sequence.
ACCESSION   DAORKW010000471 DAORKW010000000
VERSION     DAORKW010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1911_length_2956_cov_4.087211"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PLB46_14280"
     CDS             <1..867
                     /locus_tag="PLB46_14280"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRF77740.1"
                     /translation="QALGYYLALPLIYFFSILPMWVLRQASNIGYFFLFHVFKYRKEV
                     VYKNLRNSFPEKSDAEIDTIARRFYMVFADTMFETIKALTITHKQLKKKCSVKNYNLV
                     EESLNKGRNALLVCGHYGNWEWAGGSMAVHAGLPLKAAYKPLSNKYFNRLAVRSRTRL
                     KIMLIPKRQLLKHLTDTPHEQTLLVLIADQSPNPKDHYWTKFMNQDTAVVTGMERIAR
                     QYDYDVFYATIHHVSRGKYEITFKLLTNDANSLPPQQLTAMFMAELEADIRKDPGPYL
                     WSHRRWKLKKPA"
     gene            complement(864..1481)
                     /locus_tag="PLB46_14285"
     CDS             complement(864..1481)
                     /locus_tag="PLB46_14285"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF77741.1"
                     /translation="MKRILVIILALSVYQVNAQEFDLGIKGGVNYVDAVILDVVNADG
                     ISLDELNTERGKSIVFGGFARVTMGKFVFQPELLFSENQSLVKFEGIDASDESFGDLL
                     SIQVDKMDIPLIFSYKAFNAIRLSAGPVFSHLNVEAEDKLFQFNDMTVGYQAGIGFDI
                     SKLTFDARYESNLNGYQEFLSNEAGSITVDTRRSIFQFTIGYKLF"
     gene            1581..2324
                     /locus_tag="PLB46_14290"
     CDS             1581..2324
                     /locus_tag="PLB46_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRF77742.1"
                     /translation="MAKALAWDVELAPKQGKYLSGPRSRLRELTFALSVFWEFIKGFR
                     ALHFIGPCITVFGSARFDENHQYYKSARELGKKISAEGLTVLTGGGPGIMEAANRGAF
                     ESGGKSVGCNIKLPMEQKHNPYMHKWVTIKYFFVRKVLLVKYSYGFVVMPGGVGTMDE
                     FFETLTLIQTATIKNFPIVIIGKDYYKDIWEMLQKMVAARTIAVQDLNLVLFTDDIDQ
                     AVNHINFYINKHYQLKKVKPLKLLLERFV"
     gene            2354..2488
                     /locus_tag="PLB46_14295"
     CDS             2354..2488
                     /locus_tag="PLB46_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77743.1"
                     /translation="MKIPEKTQKWIKRVGVIGFLFFLLKGILWLVAGAAIIEWLKGVF
                     "
     gene            2579..>2956
                     /locus_tag="PLB46_14300"
     CDS             2579..>2956
                     /locus_tag="PLB46_14300"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF77744.1"
                     /translation="MKLLFTIFSVCVLSVFNIQAQNLTQTIRGQVTDNQSKQTLPYVN
                     IIVLNTTPTLGAVTDLDGYFEIKDVPIGRISIQATFIGYEPFVANNVELTSGKELILN
                     FELQEQLIKLDEIVISADNEKMES"
BASE COUNT          939 a          554 c          538 g          925 t
ORIGIN      
        1 caggctttag ggtattattt agcgctacca cttatttatt tcttctccat attacccatg
       61 tgggttttgc gacaagctag caatattggc tatttctttt tatttcatgt ttttaaatat
      121 aggaaagaag ttgtttataa aaatttacgc aattcatttc ctgaaaaatc tgatgccgaa
      181 atcgatacca ttgctagacg attttatatg gtatttgctg acaccatgtt tgaaacaata
      241 aaagcgctta ccattactca caagcagtta aagaagaagt gtagtgtaaa aaattacaac
      301 ctcgttgagg aatcattaaa caaaggccgt aatgcattat tggtttgtgg gcattacggg
      361 aattgggaat gggcaggagg ttcaatggct gtgcatgccg gtctgccgct gaaagccgct
      421 tataaaccgc tttctaacaa atattttaac cgtttagcag taagaagtcg gactcgcttg
      481 aaaatcatgt tgattcctaa acggcagttg ctcaaacatc ttactgatac gcctcatgag
      541 cagactttgt tagtgttaat tgccgaccaa tcgccaaatc cgaaggatca ttactggacc
      601 aaatttatga accaggacac cgcagtggtt actggcatgg agcgtatagc cagacaatat
      661 gattacgatg tgttttatgc taccatccac catgttagtc gcggaaagta tgaaattacc
      721 tttaaacttt taaccaacga tgcgaacagt ctgcccccac aacaactaac agcaatgttt
      781 atggctgaac tggaagcaga catacgcaaa gaccccggac catatctgtg gtctcacaga
      841 cgctggaaat tgaaaaaacc ggcttaaaac agtttgtaac caattgtaaa ttggaaaata
      901 cttcttctgg tatctacagt tatacttcct gcttcattgc ttaaaaattc ctgataacca
      961 ttcaggttac tttcatatcg tgcatcaaaa gtgagtttgc tgatatcaaa tccaattccg
     1021 gcctgataac cgacggtcat atcgttgaac tgaaacaatt tatcctctgc ctctacattg
     1081 aggtgactaa acacaggtcc tgctgataat cggatagcat taaatgcttt gtagctgaaa
     1141 atgaggggta tatccatctt atcaacctgg atactcagta ggtcaccaaa gctttcatca
     1201 ctagcatcaa tcccctcaaa cttcacaagt gattgatttt cagaaaataa taactcaggc
     1261 tggaaaacaa atttacccat tgtaaccctt gcaaaacctc caaaaactat tgattttcct
     1321 cgttctgtat taagttcatc taatgaaatg ccatcggcgt taactacatc taaaattaca
     1381 gcatcaacat aatttacacc acctttaata cctaaatcaa actcctgagc attcacctgg
     1441 taaacgctta aggctaaaat tattaccaaa atccttttca tatatcacac ttttcacgct
     1501 ataaaacgaa aactatgcca ttaacagtgt ttagaattta aaaacaataa aaccacttaa
     1561 ttttggcgga aacgggaatt atggcaaaag cattagcatg ggatgtggaa ttggcgccta
     1621 aacaaggcaa atatttatca ggtccgcgtt ctagattgag ggaattaacc ttcgcacttt
     1681 cagttttttg ggaatttatc aaaggctttc gggctttaca ttttattggt ccctgcatta
     1741 ctgtatttgg ctctgccaga tttgatgaaa accatcaata ttataaatct gcccgggaat
     1801 taggcaaaaa aatcagtgct gaagggctca cagtattgac cggtggtggc ccgggtatta
     1861 tggaagctgc caatcggggc gcatttgaat caggaggcaa atcggttgga tgtaacataa
     1921 aactacctat ggaacaaaaa cataaccctt atatgcataa atgggttact attaagtact
     1981 tttttgtccg taaggtgcta ttagttaaat attcgtatgg ttttgttgta atgcccggcg
     2041 gagtgggcac tatggatgaa ttttttgaaa cgcttaccct tattcaaacg gcaacaatta
     2101 aaaatttccc catagttatt ataggcaaag attattacaa agatatttgg gaaatgttgc
     2161 aaaaaatggt agctgccaga accattgctg tgcaagattt aaatttggta ttatttaccg
     2221 atgatataga ccaggccgtt aatcatatta acttttatat caataaacat tatcagttaa
     2281 aaaaagtaaa accactcaaa cttcttttgg aacgttttgt ttaatcctta actttgtatg
     2341 ttgtaagttt tatatgaaaa ttccggaaaa aacccaaaaa tggattaaac gtgtcggcgt
     2401 tatcggcttt ttgttttttt tactgaaagg catactctgg ctggttgcag gagctgcaat
     2461 catagaatgg ttaaaaggtg ttttttaatc ggttcatcca gttaatcttc ccgttcatca
     2521 tatatatatt gcagcaaatt tcaatttgat attgttttgc acttgaaaat aataatctat
     2581 gaaacttcta tttaccattt tcagtgtatg cgtgctgtcg gtgtttaata tccaggcaca
     2641 aaaccttact caaactattc gtggtcaggt taccgataat caatcgaaac aaactttacc
     2701 ttatgtcaac attattgtat tgaataccac tcctaccctt ggtgcggtaa cagatttgga
     2761 cgggtatttt gaaattaaag atgtgcctat cggacgtatt tctattcaag caacttttat
     2821 tggatacgaa ccttttgttg cgaacaatgt agaattaacc tcaggtaagg agttgatatt
     2881 aaatttcgaa ttacaggagc aacttattaa actggatgaa attgttattt ctgcagataa
     2941 tgagaaaatg gaatca
//
LOCUS       DAORKW010000472         2943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1924_length_2943_cov_3.975762, whole genome shotgun sequence.
ACCESSION   DAORKW010000472 DAORKW010000000
VERSION     DAORKW010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1924_length_2943_cov_3.975762"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1710)
                     /locus_tag="PLB46_14305"
     CDS             complement(151..1710)
                     /locus_tag="PLB46_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRF77745.1"
                     /translation="MKKLTTKIFLGTAAVTGLLLTQSCEKEIPAYNGFESYTYASLDE
                     DGGTWDPILLTSGADTAIAAPADVTSDAYLAELDEVKGYVGSLNADEQEAVDYWGNNT
                     VIRWQEIAQELVAKYNLAPSPNEDGSYSSPSSANPSAYPNFPFAHPPYAVRAYAYLAA
                     AQYDALITTWNYKYAYNRPAPYKVDGSITPAYPDNNLPSYPSEDAAIAEVSSEMLKFL
                     FPLEVDYINGLAQECRESRKWAGMNVESDLVAGDGIGGYVFRQYKARAANDSMKFAQI
                     DAATYAGYESAADLMFGNMWPHWENLEVPQRPVGITPAYGKVKTWWIGSPADVRPGPP
                     PAVGSAEYETAKAELLEYTKNATREQEQMAYFWGDGFGSYTPPGHWDRIAADYIVQYR
                     LNPLRTARTFAYLNTAMMDAGISCWDTKYYYMYPRPPQGNEDILCLFGLPNFPSYTSG
                     HSTFSAAAATVLGYIFPSEASLFSSYAQDAADSRVYSRIHWRFDSEVGLDVGQTIGAM
                     TVAVAATDGAD"
     gene            complement(1712..2683)
                     /locus_tag="PLB46_14310"
     CDS             complement(1712..2683)
                     /locus_tag="PLB46_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77746.1"
                     /translation="MYLNRLLKSTLTLLVISATVGHVNAQTPGDAVMMNAGEICFDVH
                     YNNAAWSKYWEGDSLRENGNIGTLTTQSITGGFMLGILDRVNVLAMLPYVITDPSGGV
                     VSGDKGIQDAAFFVKGLIYEHKAGPGNIKFLASAGLLLPASDYSPENPFAIGLGCPDG
                     IFRGIVHYDADMGLYGRVDGAFHLRGNAFLDRNYYYTTSGYYSDEVDMPDAMDYNFTL
                     GYITKSKKFKVEGALNSLTTFGGFDIRRQDGGFPSNDMEFMRIGLNADYYDLLVKGLA
                     VHFNSNYTLTGRNVGKSMMFGGGISYQFGLWGKKDKAQSSSPTPQAN"
BASE COUNT          898 a          637 c          555 g          853 t
ORIGIN      
        1 agcacccaag tgttattcgc gagcgagctc cctcatggga cacaaattgt ggactttaat
       61 gctgtttgaa cttattatat tttaacaaaa aaaaaacgag gctattgatt tacttcaaca
      121 gcctcgttta caattatatt tataaaacca tcaatcagcg ccgtcggtgg cagcaactgc
      181 aacagtcatt gctccaatag tttgaccaac atctaatcca acttcactat caaacctcca
      241 atgtattctt gaataaactc gtgagtccgc agcatcttga gcgtatgaac taaacaaact
      301 tgcttctgac ggaaatatat atcctaatac cgttgcagca gcagctgaga aagttgagtg
      361 acctgaagta tacgaaggga aatttggcaa gccgaataaa cataaaatat cttcattgcc
      421 ttgtggaggt cgaggataca tataataata tttggtatcc caacaactaa ttccggcatc
      481 catcatagca gtgttcaaat aagcaaatgt acgtgctgtg cgcaatggat ttaaacgata
      541 ttgcactata taatctgctg caattcggtc ccaatgtccc ggtggtgtat aagatccaaa
      601 gccatctccc caaaaatacg ccatttgttc ctgctcacgg gttgcatttt tagtatattc
      661 caagagttct gcttttgcag tttcatattc cgcagaacct actgctggtg gcggaccagg
      721 gcgaacatca gcagggctgc caatccacca tgttttaact ttgccatatg caggtgtaat
      781 accaaccgga cgctgaggaa cttctaaatt ttcccaatgt ggccacatat ttccaaacat
      841 taaatcagca gctgattcat atcctgcata tgttgcagca tcaatctgcg cgaatttcat
      901 actatcgtta gctgcacgtg ctttgtactg acggaaaaca tacccaccaa tgccatcacc
      961 cgcaactaaa tcgctctcca cattcatacc tgcccatttg cgagattcgc gacattcctg
     1021 agcaagtcca ttgatataat caacttctaa tggaaacaaa aatttgagca tttcagaaga
     1081 aacctctgca atagctgcat cctcagaagg ataagatggt aaattattat ccggataagc
     1141 cggggttata ctgccatcta ctttatatgg agcagggcga ttataagcat atttatagtt
     1201 ccatgtggtt attaatgcat catattgcgc cgctgccaaa tatgcataag cacgcacagc
     1261 ataaggcgga tgtgcaaaag ggaaattagg ataggcagaa ggatttgcag agctgggaga
     1321 gctgtatgat ccatcctcat tcggagatgg tgcaagattg tattttgcta ccaattcctg
     1381 agcaatttcc tgccagcgaa ttacggtatt atttccccaa taatcaactg cctcttgttc
     1441 gtctgcatta agtgaaccaa catatccttt cacttcatct aactcagcta aatatgcgtc
     1501 tgaagttaca tctgcaggag ctgcaatagc cgtgtcggcg ccgctggtta ataaaatggg
     1561 atcccaggtt ccaccatctt catctaaaga tgcataggtg tatgattcaa agccgttgta
     1621 agcgggaatt tccttttcgc aagactgcgt tagcaataaa cctgttactg ctgcagtgcc
     1681 taaaaatatt tttgttgtaa gttttttcat gttagtttgc ttgaggggtt ggtgaagaac
     1741 tttgtgcttt atcttttttg ccccagaggc caaattggta agatatacca ccaccgaaca
     1801 tcatagattt acctacgttg cggccggtta aggtatagtt tgagttgaaa tggactgcta
     1861 aaccttttac caataaatca tagtaatcgg catttaaacc tatgcgcata aattccatat
     1921 cgtttgatgg aaaaccgcca tcctgacgtc tgatatcaaa tccaccgaag gtagtgagtg
     1981 aatttaatgc gccttccact ttgaattttt tgcttttggt aatatatccc aatgtaaaat
     2041 tgtaatccat tgcatccggc atatctactt catcagaata ataacctgag gtagtataat
     2101 aatagttgcg atcaaggaat gcatttcctc ttaaatggaa agctccatct acacggccat
     2161 aaagtcccat gtcagcatca taatgcacga taccgcgaaa aataccatca gggcaaccta
     2221 agccgattgc aaaagggttt tcaggagaat aatcagaagc aggtaataac aaccctgcgc
     2281 tggctaaaaa tttgatatta cccggaccgg ctttgtgttc atatataagc ccctttacaa
     2341 aaaaagcagc gtcctgaata cctttgtcgc ctgaaactac accacctgac gggtctgtaa
     2401 ttacataagg caacatagct aatacattaa cacggtctaa tattccaagc ataaaaccac
     2461 ctgtaatgga ttgagtagta agtgtgccga tattaccgtt ttcacgaaga gaatctccct
     2521 cccaatattt gctccaggct gcattattat aatgcacatc gaaacaaatt tcacctgcgt
     2581 tcatcattac tgcgtcaccg ggggtttgcg cattaacgtg accaacagtt gctgaaatta
     2641 ctaacagcgt aagcgttgat ttgagtagtc tgtttaaata catattttgt ggattttggg
     2701 caaaagtagt tgcacaatga taaagctaag tcccatttaa agtagtttta aagagtagtt
     2761 ccctcgttta aaaaacttta gcacttgatt gtgaacctta gcattccatg gacataaaaa
     2821 cataaaatat gacttgcatt tcctttttag aggatagatg tcattttaaa cgcaacctac
     2881 catgcctacc ttcggaatta ctaaaacata atagtatgga cactgatcaa attttatctg
     2941 caa
//
LOCUS       DAORKW010000473         2942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1926_length_2942_cov_4.926221, whole genome shotgun sequence.
ACCESSION   DAORKW010000473 DAORKW010000000
VERSION     DAORKW010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1926_length_2942_cov_4.926221"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLB46_14315"
     CDS             <1..665
                     /locus_tag="PLB46_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77747.1"
                     /translation="HISVIAWNNSDNYSPFTTVDKICNAIFNFDTTKTVSDMLPGFNQ
                     KTLEGIYLTNNNLIREVKVKGDSLILRLPLQQSTRFHTLTYTGSFRDSIITYIDSLNN
                     PVTFVKSAGRVVGFNWQGGEYFVANRYYSKIDENKIIPTEQLTGKYENGQQNKKVKVS
                     YKKYRHQLYLRNFIFYKMKMEHVCGNVYYLPEYQFYVRAESDKIILGDDWIFNLTYTK
                     NK"
     gene            complement(714..>2942)
                     /locus_tag="PLB46_14320"
     CDS             complement(714..>2942)
                     /locus_tag="PLB46_14320"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77748.1"
                     /translation="ISNGESVTDWAWGDGAGGGGAGGSVIISAADATDLNIIAKGGKG
                     GDTGADQCTGPGGGGSGGAFKFNAGAVWAGVTTDLSGGIYGTNTTAISDCFGNNNGAA
                     SGGTGVSVANFILSESLIAYNPDFADAGADVLVCSGSSVNLDGSGGTTYSWSPGTYLD
                     NPLIANPLCTPGGDITYTLTVTNGSGCTDTDVITVAIAPEVIADAGEDVVKCGDEGVM
                     LNASGGVSYSWSPTAFLDNPLIANPISTAPIDFNYMVTVTDINGCTGTDVVSVLISTS
                     NFLTTTGVAGVCAGGAVGLLAEGGTTYSWSPSTYLDNPLIANPICTPLADITYYVTST
                     NDEGCEDIDTVVVTIIPSEFLSLDDDVSVCEGTSVPLLATGGTTYSWSPSTYLDNPLI
                     ANPVCTPLSDITYIVTSSSIEGCIDTDTIHISVLPGNFANAIPDVNVCAGDPTTLMAA
                     GGVTYEWSPTTYLDDPFSDEPICTPLSDITYTVTVTNADGCVDEETVNVTTIPVEPVT
                     AGPDTAACSAGQIFLFVSEGESYEWTPATYLDNSAIQFPTSNPVSSISYIVYVTDING
                     CVGSDTVDIIIYDNPIVTASEDVTICRGESVIISASGATTYSWFPEPITPCGDCESIT
                     VSPTETTTYSVQGFDANGCYDQAEVTVTVEICNAIHDELFGEIKVYPNPAKQYIVITL
                     PTILADADFVLLNMLGQQLNTTILRQQQNTLIEFESMPAQQLMLQIKTQYGIVYKSIT
                     VE"
BASE COUNT          947 a          620 c          523 g          852 t
ORIGIN      
        1 aacatatttc ggtaatagcc tggaataatt cagacaacta ttcaccattt actacggtag
       61 ataaaatttg taatgcaata tttaatttcg acacaacaaa aaccgtttcg gatatgctcc
      121 cgggttttaa tcaaaaaaca ctggaaggga tttatcttac caacaataat ttaattcgcg
      181 aagtgaaagt aaaaggtgat agtttaattt tgcgacttcc attgcaacaa agcacccgtt
      241 ttcataccct cacctataca gggtcgttca gagatagtat aattacttat attgactcat
      301 taaacaatcc ggtaactttc gtaaaatcag ccggccgtgt tgtgggattt aattggcagg
      361 gaggtgaata ttttgttgcc aaccgttatt attcaaaaat tgatgagaat aaaattattc
      421 ctaccgaaca attaaccggt aaatatgaaa acgggcaaca aaataaaaaa gtaaaagtta
      481 gttataaaaa ataccggcat caattatact tgcgcaattt tatcttttac aaaatgaaaa
      541 tggaacatgt ttgcggcaat gtgtattatt tacccgaata ccaattttat gtaagagcgg
      601 aatctgataa aattattctt ggggatgatt ggatttttaa tctcacctac actaaaaata
      661 aataaagccg gacaaacaca attgttatcc ggccttataa tattgtattg attttattct
      721 acagttattg atttatacac tattccatat tgagttttaa tctgtaacat taattgttgc
      781 gccggcattg actcaaattc tattaatgta ttttgttgct ggcgtaaaat agtagtgttt
      841 aattgctggc ctaacatatt taataacaca aaatcagcat cagcaagtat ggtaggtaat
      901 gttatcacaa tatattgctt tgccgggttt ggataaactt taatctcccc aaataattca
      961 tcatgaatgg cattacatat ttcaactgtt accgtaactt cagcctgatc gtaacaacca
     1021 ttggcatcaa aaccctgaac ggaataggtg gtagtttctg ttggagaaac agtaattgat
     1081 tcgcaatcac cgcaaggtgt aataggttcc ggaaaccaag aataggttgt tgcaccactt
     1141 gcgctaataa ttacagattc accacgacaa atagttacat cttctgaagc agttacaatt
     1201 ggattgtcgt aaataataat atcaactgta tcggaaccaa cacatccgtt tatatcggta
     1261 acgtaaacaa tataactaat ggatgaaaca ggattactgg ttggaaattg aatggcacta
     1321 ttatctaaat aagtagccgg agtccattcg taactttccc cttcacttac aaataaaaat
     1381 atttgtcctg cactacaagc tgctgtatca ggacctgcag taacaggctc tacaggaatg
     1441 gttgttacat taacggtttc ttcatctaca catccatcag cattggttac agtaacagta
     1501 taggtaatat cacttaatgg tgtacaaatt ggttcatcgg aaaatggatc atccaaataa
     1561 gttgttggac tccattcata cgttacacca cctgcagcca ttaaagtagt tggatcacct
     1621 gcacaaacat ttacatctgg aattgcattc gcaaaattac ctggcaacac tgaaatatga
     1681 atggtgtctg tatcgataca accttctatt gaagatgaag taacgatata agttatatct
     1741 gataatggtg tgcaaacagg attggcaatt aaaggattat ctaaatatgt tgaaggactc
     1801 catgaataag ttgttccgcc tgtagctaat aatggtactg atgttccttc acaaactgaa
     1861 acatcatcat ccaacgataa aaattctgat ggtataattg tgacaacaac agtatctata
     1921 tcttcacaac cttcgtcgtt ggttgaggta acataataag taatatctgc cagcggcgta
     1981 caaatcggat tggcaattaa tggattatct aaatatgttg atggcgacca actgtatgta
     2041 gttccacctt cagcaagtaa accaactgca ccacctgcac aaacacctgc tacaccggtt
     2101 gttgttaaaa aattagatgt gctaatcagc accgatacta catcagttcc agtgcaaccg
     2161 tttatatcgg ttaccgttac catataatta aaatcgatag gtgccgtaga aatcggatta
     2221 gcaattaacg gattgtcgag aaatgccgtt ggcgaccaac tatatgaaac accaccactt
     2281 gcatttagca ttacaccttc atcaccacat ttaactacat cttcacccgc atccgcaata
     2341 acctccggtg caatagcaac tgttattaca tcagtatctg tgcatccgga gccgttggtt
     2401 acggtcagtg tataggtgat atcaccacct ggcgtgcata atggattggc aatcaaagga
     2461 ttgtccaaat acgttcccgg cgaccagcta taagttgtgc ctccggaacc atccaggtta
     2521 actgaacttc cgctgcaaac taaaacatca gctcctgcat ctgcaaaatc gggattgtaa
     2581 gcaataaggc tttctgaaag tataaaattg gcaactgaaa ccccggtgcc acctgaagcg
     2641 gcgccattat tattgccgaa gcaatcgctg attgccgttg tattggtacc ataaattccg
     2701 cctgacaaat cagtagtaac acctgcccaa accgcgcctg cattaaattt gaatgcacca
     2761 cctgaaccgc cacctcccgg tcctgtgcac tggtcagctc cggtatcgcc accttttcca
     2821 ccttttgcta tgatattgag gtcagttgcg tcggcagcac taataatcac cgaaccacct
     2881 gcaccaccgc caccggcacc atctccccaa gcccagtccg taaccgactc accattactg
     2941 at
//
LOCUS       DAORKW010000474         2931 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1936_length_2931_cov_4.721140, whole genome shotgun sequence.
ACCESSION   DAORKW010000474 DAORKW010000000
VERSION     DAORKW010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1936_length_2931_cov_4.721140"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            419..2677
                     /locus_tag="PLB46_14325"
     CDS             419..2677
                     /locus_tag="PLB46_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77749.1"
                     /translation="MANHAGFGGVLTISTTFAQQILHAYYSSDNVTPILQSESPLKLL
                     AKTDNNNIPLPDRYLSYDLFMAEPILDFSPREDDKISIFIRLSGTLTFSTDPELALPT
                     IDSNVQIDFTIPANVRTFDNGEQISFGITFDSCFIETYQAKVFNGTDPKTDYLFDVNI
                     DLKSFIQLAIFGLDTSTWRITPPGFDEIKSLGLSVSEFPEIKLFDVALTIAIDVVGET
                     SGNQNLLIDLLNSSLEKGYITTFSDTIHQFDDEYGEPIYDTGFTATTKRQAGPHNLCA
                     IAFSVNGTIINMLYEGVWRQLILDAFEAQKAQAIADALAEALEKNKKYDEPDIANIEI
                     TDINLSLEDSHIFVSGDADYSGIGVSFSFSIRVVKINVDGSTQFVYDNQHMNGMRGEV
                     FDVDINLPGWVVFLQAVIGTLGIVLAPFTGMISALIALMLEVTIGAILTNVTENAEGD
                     IKNDIMNQLASFNSTFTFTLPNTESPEITVRPNDIVVKANGLSTWFTALVDYGKFTEL
                     IISGNNNSTIWSVHNRNPIVIKFSAPEGLYHPDDEMVKIRWTVFAEHTGNKIIETDEP
                     IKRKLLSPHIPTHCKIDHAKTEWDAFEKYIVRCRVYRPWGVTTQEIYYKEIVITIADR
                     LHRDQLYVRWESAVFYKGYTAKLKDENRQPLGWINEKRKSQIHKTDPDTRCGYADRYT
                     DKLTKDELIYLDALPFPESDIKLHLNEICEFCFYGGPDKVNPKPPKIPKDEAPGKKPH
                     VPDHFKKKKPFH"
     gene            2746..>2931
                     /locus_tag="PLB46_14330"
     CDS             2746..>2931
                     /locus_tag="PLB46_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77750.1"
                     /translation="MSELQYIQLSELIQKVEDTLRGVFGNEQYWIVAEISGHKFYANN
                     DRHYFEFIEKSSTSNEPV"
BASE COUNT          974 a          514 c          550 g          893 t
ORIGIN      
        1 atcaaccgca ttgcaatgtg gagcaaaacc tcctggaatc tacaaaaata gattaaacaa
       61 tatttagcat aaaaacacaa aataattgta actcgggcaa aatttttcaa tcaaactatt
      121 tcatttccaa ctacatatat tgtaaggggt taaaatatca tctttttact tgtaaaatca
      181 taactgattc cctaacttta aaatagaata tcgggtctcc acaagaaccc gtatgcaaaa
      241 acagaagaag agaaaatttg taaatggttc cagttggtcg ttcgatgcag cgcatcgtgt
      301 ccgttctgcc ctgattgcaa tgtccttcga attgtacggc attcagaaat gaatacgttt
      361 aaactgcatt acatgttcca cccactccga aaggagtggg tttttaaacc ttaaaactat
      421 ggccaatcac gccggatttg gaggagtgct tacaatcagc accacatttg ctcagcaaat
      481 tttgcatgct tattacagta gcgataatgt tacgccgatt ttacagtctg aaagtccgct
      541 taaattatta gccaaaacag ataataacaa tataccactg cccgaccggt atttgtcata
      601 cgatttattt atggcagaac ccatccttga tttttcacca cgtgaggatg ataaaatttc
      661 tatttttatt cgcttgagcg gcacgttaac attttcgacg gacccggaat tggcattacc
      721 aacaattgat agtaatgttc aaattgattt taccattccg gctaatgtgc gcacatttga
      781 taacggtgaa caaatttcat ttggtattac attcgatagc tgctttattg aaacttacca
      841 ggccaaagtt tttaatggta cagatccgaa aaccgattat ttgtttgatg taaatattga
      901 tttaaaaagt tttattcaat tagcaatttt tggtttagat acatcaactt ggcgcattac
      961 gccgccgggc tttgatgaaa taaaatcact gggattaagt gtctcggaat ttccggaaat
     1021 taaattgttt gatgttgcat taacaattgc cattgatgtt gttggtgaaa ccagtggcaa
     1081 ccaaaattta ttaattgact tgctgaatag ttcactggaa aagggatata tcaccacttt
     1141 tagtgatacc attcatcaat ttgatgatga atatggtgaa cctatttacg ataccggctt
     1201 cacggcaaca acaaaacgac aagcaggacc gcataattta tgtgctatag cattttcagt
     1261 aaatggtacc atcatcaata tgttgtatga aggtgtatgg cgacaattga tattggatgc
     1321 atttgaagca caaaaggcac aggcaattgc tgacgcgctt gcagaggctt tggaaaaaaa
     1381 taaaaagtat gatgaacctg atattgccaa tattgaaatt acagatatca acctgtcgct
     1441 ggaagacagt catatttttg tttccggtga tgcggattat agtggcattg gtgtttcatt
     1501 tagctttagt attcgggtgg tgaaaataaa tgttgatggc tcaacacaat ttgtttacga
     1561 taatcagcat atgaacggta tgcgtggtga agtatttgat gttgatatta acttaccggg
     1621 atgggtggtg tttttacaag cagttatcgg tacattagga attgtattag caccatttac
     1681 gggtatgatc agtgcactta ttgcattaat gcttgaagtt accatcggtg caattttaac
     1741 aaatgtaaca gaaaatgctg aaggtgatat caaaaatgat attatgaatc aacttgcatc
     1801 attcaacagc acatttacgt ttacattgcc aaatacggaa agcccggaaa ttactgtgcg
     1861 tccaaatgat attgtagtga aagcaaatgg tttatctaca tggtttactg cactggttga
     1921 ttatggtaaa tttactgaac ttattatttc cggaaataat aattccacaa tttggtcagt
     1981 acataaccgc aatccaatcg tgataaaatt cagtgcccca gagggacttt atcatccgga
     2041 tgatgaaatg gtaaaaattc gctggaccgt ttttgcagag catacaggaa ataaaattat
     2101 tgaaacggat gaaccaatca aaagaaaatt attatctcca catattccaa cccattgtaa
     2161 aattgaccac gccaaaacgg aatgggatgc ttttgaaaaa tatatcgttc gttgtcgagt
     2221 ttacagaccc tggggtgtaa caacacaaga gatctattat aaagaaattg taatcaccat
     2281 tgcagaccgc ctgcatcgtg atcaactata tgtgcgttgg gaatccgcag ttttttataa
     2341 aggatatact gcaaaattaa aggatgaaaa tcgccagcct ttaggttgga ttaatgaaaa
     2401 aagaaaatca caaattcata aaacagatcc ggataccaga tgcggttatg cagatagata
     2461 taccgataaa ctaaccaaag atgaattaat ttatctcgac gcattaccat ttccggaatc
     2521 agatataaaa ttgcacctaa atgaaatttg cgaattttgt ttttacggtg gtccagataa
     2581 agtgaatcct aaaccaccga aaataccaaa agatgaagct cccggcaaaa aacctcatgt
     2641 tcctgaccat tttaaaaaga agaaaccttt ccattaatca ggttttgaaa tttgctgtaa
     2701 ttccaatttg aattgtttac cttagcaggt caactttgca gcaacttgtc cgaattacaa
     2761 tatatccaac tttccgaact catccaaaag gtagaggata ctttgcgtgg tgtttttggc
     2821 aatgagcaat actggattgt tgcagaaatt tcggggcata aattttatgc gaataacgac
     2881 aggcattatt ttgagtttat tgaaaaatcg agcacatcaa atgaacctgt t
//
LOCUS       DAORKW010000475         2929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1939_length_2929_cov_4.714335, whole genome shotgun sequence.
ACCESSION   DAORKW010000475 DAORKW010000000
VERSION     DAORKW010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1939_length_2929_cov_4.714335"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..704)
                     /locus_tag="PLB46_14335"
     CDS             complement(120..704)
                     /locus_tag="PLB46_14335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRF77751.1"
                     /translation="MGSINNPKKFIFIAGFVTLIGIGGLGLLLIYFFHKEVWFSILNV
                     SIESLLRQCITGTLYALVTLIPLVFLLERRMLDATRYFFADLMKKFTVSTPEIFLLSL
                     CAGVGEELLFRGAIQPWIGIWPTAIIFIALHGYLNPKDRPMFIYGMVLLLVSAGFGYL
                     LPFSGIYAAMTAHFWIDVVLMVYLKRNSGKHFVV"
     gene            complement(692..1693)
                     /locus_tag="PLB46_14340"
     CDS             complement(692..1693)
                     /locus_tag="PLB46_14340"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HRF77752.1"
                     /translation="MNITKKLLLLLFVTGLVFTACKKNVSDDKDKTLNDTTATTSNDT
                     SVSINLNSPVADEKILLRFQPVVGTTYIVESNVNSTMSEAQDTFKINYKSNKFGKVSL
                     RILSKENTDYKMEFILKDVRETIKADTSTIEYKYGKALADPQADMNRKIEDCLVNSPL
                     TIMMSSAGEATDIRGYEGIIAKVKAIVGQNIDDNMIAAQLGTPTETLENYFINYPDSA
                     VKIGESWDLTVASQMQGVPIVLINTYTLADRKDGVAFINFRTDIEIDKSQIPAEVAAQ
                     MSNIKFDAYIKGTGEVDEKTGWPKVIKVTQSVELSDSFEGHVTSSKQYSTSVIKWVQ"
     gene            1755..>2929
                     /locus_tag="PLB46_14345"
     CDS             1755..>2929
                     /locus_tag="PLB46_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRF77753.1"
                     /translation="MQISANHKKSFAEAYASLNDNQRKAVDTIEGPVLVVAGPGTGKT
                     QILAVRIGKILMETDTLPDNILCLTYTDAGTIAMRKRLLDFIGPDAYRVHIYTFHAFC
                     NDVIQANLDYFGKRELEPISELENVNLIEQLLEELPLNHPLKKLKGNKSYEVGRLNNF
                     FKMMKDEDWDEQKINQTIDAYIADLPNREDYIYKRANAKTGVKVGDVKQKELQAEVDR
                     MEKTRAAANMYPEYVSRMRAMGRYDYSDMILWVIKAFRDTSPAGENMLRNYQERYLYF
                     LVDEFQDTNGAQNEILNLLISYWDIPNVFAVGDDDQSIYEFQGARVKNIMDFYQSYER
                     EIEVVVLTDNYRSHQAILDASRVIIEKNEERLINKLKNLSKVLTAAHPNRQHITKPA"
BASE COUNT          965 a          555 c          528 g          881 t
ORIGIN      
        1 aaaatagcta agagcattac acttttccgc ttcatatatc gtattgtttt ttcctaagtt
       61 aatttaaaaa aatgattatg ttttgaattg agatacagct acagggcaaa ttgcataaat
      121 taaacaacaa aatgtttgcc gctattgcgt ttcaggtata ccataaggac tacgtcaatc
      181 caaaaatggg cagtcatagc agcatatatt cctgaaaacg gcaacagata gccgaaaccg
      241 gcactcacca gcagcaaaac catgccataa ataaacattg ggcgatcttt gggatttaaa
      301 taaccatgca gggcaataaa aattatggct gtaggccaaa tgccaatcca gggttgaata
      361 gcaccccgaa acaacaattc ttcacccact cctgcgcaca atgaaagtaa aaatatttcc
      421 ggtgtgctga cggtgaattt tttcatcaaa tccgcaaaaa aatacctggt agcatccagc
      481 atgcgtcgct ccagcaaaaa caccagcgga attaaggtta caagtgcata caacgttccg
      541 gtaatacatt gacgcaacag cgattcgatg ctaacattta atatcgaaaa ccaaacctct
      601 ttatgaaaaa aatagataag tagtaagcca aggccaccaa taccaattaa ggtgacaaat
      661 ccggctatga aaataaattt tttaggattg tttattgaac ccacttaatt actgatgtac
      721 tatattgttt agaagaagta acatgtcctt caaagctatc agaaagttca acactttgcg
      781 ttactttaat cactttaggc caaccggttt tttcatccac ttcgccagtg cctttaatat
      841 aagcgtcaaa tttaatgtta ctcatttgtg cggcaacttc tgccggtatt tgcgatttat
      901 caatttcaat gtctgttctg aaattaataa aagcaactcc atctttcctg tctgccaaag
      961 tatacgtatt aattaataca ataggcacgc cctgcatttg tgaagccaca gtaaggtccc
     1021 agctttcgcc aatttttaca gcagagtcgg gataattaat aaaataattt tccagtgttt
     1081 cggtaggtgt tcccaattga gcggcaatca tattgtcatc aatgttttgg ccgacaatag
     1141 ctttaacttt agcgataatt ccttcataac ccctgatatc agtggcttct ccggcgctcg
     1201 acatcataat tgttaatgga gaatttacca agcagtcttc aatttttcta ttcatatcgg
     1261 cttgtgggtc agccaatgct ttgccatatt tatattcaat agttgaagtg tctgctttaa
     1321 tagtttcgcg aacatctttt aagataaact ccattttata gtcggtgttt tcttttgaaa
     1381 gaatacgcaa actaactttt ccaaatttat ttgacttgta atttattttg aaggtgtcct
     1441 gtgcttcact catggtggaa tttacattac tttccacaat ataagttgta ccaactaccg
     1501 gttgaaaacg caataatatt ttttcatctg ccaccgggct gttcaaatta atggaaacgc
     1561 tggtatcatt acttgttgtt gcagtggtgt cgtttaatgt cttatcttta tcatcagaaa
     1621 catttttttt acaggcagta aaaacaagtc ctgtaacaaa taacaacaac aataattttt
     1681 tagtaatatt catttttcaa ttttacattt gtttgcggca aattgcccac aaatgcaaaa
     1741 ctacgccaaa taccatgcaa atttcagcca accataaaaa atcatttgca gaagcatatg
     1801 cttcgttaaa cgacaatcag cgcaaagcgg ttgatactat tgagggtccg gttttggtgg
     1861 ttgcaggtcc gggaacagga aaaacacaaa ttttagctgt gcgaataggc aaaattttaa
     1921 tggaaactga cacgcttccg gataatattc tctgtttaac gtataccgac gccggcacca
     1981 ttgccatgcg taaacgttta ctcgatttta ttggtcccga tgcttatcga gtgcatattt
     2041 acaccttcca cgcattttgt aatgacgtaa ttcaggctaa cctcgattat tttggtaaac
     2101 gtgaacttga acctatttct gagttggaaa atgttaacct gatagaacaa ctgttagaag
     2161 aattacctct taaccatccg ttaaaaaaat tgaaaggcaa taaaagttat gaagtaggac
     2221 ggctgaataa tttttttaaa atgatgaagg atgaagattg ggatgaacaa aaaataaatc
     2281 aaacaattga tgcctatatc gccgatttac cgaacagaga agattatata tataaacgtg
     2341 caaatgccaa aacaggtgta aaagttggtg atgtaaaaca aaaagaattg caggctgaag
     2401 ttgaccgtat ggaaaaaaca cgggctgctg ccaatatgta tcccgaatat gtatcacgca
     2461 tgcgcgccat ggggcgatac gattacagcg atatgatttt atgggtgata aaagcctttc
     2521 gcgacacttc acctgcagga gaaaacatgt tgcgcaacta tcaagaaaga tatttatatt
     2581 ttttggtgga cgaatttcaa gataccaatg gtgcgcaaaa tgaaatactg aatttattaa
     2641 ttagttattg ggatatacca aacgtatttg ccgttggaga cgacgaccaa agtatttatg
     2701 aatttcaggg agcccgtgta aaaaatatca tggattttta tcagtcgtat gaacgtgaaa
     2761 ttgaagttgt tgtgcttacc gataattatc gctcacatca ggcgatttta gatgcgtctc
     2821 gtgtgattat tgaaaaaaat gaagagcgac tgatcaataa attaaaaaat ttatcgaaag
     2881 tacttaccgc agcgcatccc aacagacaac atattaccaa acctgccat
//
LOCUS       DAORKW010000476         2922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1943_length_2922_cov_4.428322, whole genome shotgun sequence.
ACCESSION   DAORKW010000476 DAORKW010000000
VERSION     DAORKW010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1943_length_2922_cov_4.428322"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLB46_14350"
     CDS             complement(<1..718)
                     /locus_tag="PLB46_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRF77754.1"
                     /translation="MIEITIPLDGIRPIDFYGVHNARLGIIKKTFPYLNISGRGSELK
                     VRGNEKDIDDFKSKINKVLIHLEKFGSISDTNLQSILFGIAEDELVDLPELPDDAIVH
                     GQNGFLVRARTPNQKKMVALANENDIVFAIGPAGTGKTYTAVALAVRALKDKVVKRII
                     LTRPAVEAGENLGFLPGDLKEKIDPYLRPLYDALDDMIPADKLGYYMSSRTIEIAPLA
                     YMRGRTLDNAFIILDEAQNAT"
     gene            795..1568
                     /locus_tag="PLB46_14355"
     CDS             795..1568
                     /locus_tag="PLB46_14355"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRF77755.1"
                     /translation="MPIVTLTSDLGVKDYYVAIVKGKILSRVPSAQLVDITHEITPFN
                     IQEAAFVLKNTFPHFPAKSIHLVSVHAETRTDTKVVVLEHENHFFVGMDNGLFALMIE
                     GAPKQIVVVPVPENLNTSFMAKDVMSGVVADLLEGKPLSSIGGPLNALESKTYLRPPD
                     NAFIMRASIIYIDRFGNLITNVTKSQFEKIRDGRNFVINYKRNEELREISGTYSEVPE
                     GERLCLFNSSGNLEIAINKGNASQLLGLHLDNMIQIEFE"
     gene            1595..1852
                     /locus_tag="PLB46_14360"
     CDS             1595..1852
                     /locus_tag="PLB46_14360"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRF77756.1"
                     /translation="MPDKTDEFIRIFMGAQHKIEAFPGCKGVDLTRDIMQPNIFITLS
                     LWDSIESLDNYRNSELFKTTWAQTKIMFSERPDAWSLDKLM"
     gene            1854..2918
                     /gene="aroB"
                     /locus_tag="PLB46_14365"
     CDS             1854..2918
                     /gene="aroB"
                     /locus_tag="PLB46_14365"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRF77757.1"
                     /translation="MQISPDNYTIYIQPTLDDSGVFTSWLNQHQYSNVFVLCDDNTLR
                     YCYPQIKQFLPSHSCITIPAGESEKNIETCKNLWQQFTDAQADRKSLLINLGGGVIGD
                     MGGFVASTYKRGFDFINIPTTLLSQVDASVGGKLGIDFNGVKNIIGLFQNPKAVFISP
                     EFLPTLPFEQLRSGFSEILKHGLIYNAEYWHQQKGKTVEPNSDWLAAIEQSVLIKHEV
                     VKKDPFESGLRKILNFGHTIGHAIETWSLQNETQPLLHGEAIAIGMICEAWLSNAVNG
                     LPADELEEITHIFLQLFGKYPLEKVPLAAIMDVMQQDKKNSNKTIRFSLLHAIGNCDY
                     DIQVSDGLIADALTYYCTLS"
BASE COUNT          904 a          524 c          558 g          936 t
ORIGIN      
        1 cagtagcatt ttgggcttca tctaaaataa taaatgcatt atctaatgtt ctgcctctca
       61 tatatgctaa tggtgcaatt tcgatagttc tggaactcat atagtatccc aatttatcgg
      121 ccggtatcat atcatccaat gcatcatata aagggcgcag gtaagggtct attttttctt
      181 ttaaatcgcc aggtaaaaat ccgaggtttt cacctgcttc tacagcaggg cgggttaaaa
      241 tgatacgttt tactacttta tcttttaacg cacgaacggc caacgcaacc gcagtgtagg
      301 tttttccggt tccggcagga ccaatggcaa aaacaatatc attttcattg gctaaagcca
      361 ccatcttttt ctgatttggt gtccgggccc ggactaaaaa tccgttttgg ccatgcacaa
      421 tcgcatcgtc aggtagttca ggtaaatcta ccagctcatc ttcagcaata ccgaatagta
      481 tactttgtaa attagtatca ctaatactcc caaatttttc gaggtgaata agtactttat
      541 tgattttact tttaaaatcg tcaatgtctt tttcattgcc tcttaccttc aattcgcttc
      601 cgcgacctga aatattgaga taaggaaagg tttttttaat gatccccaac cgcgcattat
      661 gtacaccgta gaagtcaatc ggccttatac cgtctaaagg aattgttatt tcaatcaatt
      721 tttcttataa ttttgtgctg ttcaatgcaa atttaatttt tttgacttaa cccactcgtt
      781 ttacctaaat aattatgcca attgtaaccc taacctccga ccttggtgta aaggattatt
      841 atgtggccat agtaaaaggt aaaattttgt cacgagttcc ttcggcacaa cttgtggata
      901 tcacccatga aattacgcct tttaacattc aggaagctgc ttttgtgtta aaaaatacat
      961 ttccccattt tccggctaaa agtattcatt tggtaagtgt gcatgcagaa accagaactg
     1021 atactaaagt ggtagtgctt gagcatgaaa accacttttt tgtcggcatg gataatggcc
     1081 tgtttgcatt aatgattgaa ggtgctccaa aacaaatagt ggttgtcccg gtacccgaaa
     1141 atctcaacac atcatttatg gcaaaagatg taatgagtgg ggtagtggcc gacctgttgg
     1201 agggtaaacc gctgagttca attggggggc cgcttaacgc gcttgaatca aaaacttact
     1261 tgcggcctcc ggacaatgct tttataatgc gtgcaagtat catatatatc gaccgttttg
     1321 gcaatttgat taccaatgta acaaaatcac agttcgaaaa aattcgggat ggacgcaatt
     1381 ttgttataaa ttacaaaaga aatgaagaac ttcgcgaaat atccggcaca tatagtgagg
     1441 ttccggaagg tgaaagactt tgtttgttca actcctcagg taatttggaa attgctatca
     1501 ataagggcaa tgccagtcaa ttactcgggt tgcatctgga taacatgata caaattgaat
     1561 ttgaatgata atacgcattg ttaagatgac ttttatgccg gataaaactg atgaatttat
     1621 tcgcatattt atgggagcac aacataaaat cgaggcattt cccggttgta aaggggtcga
     1681 tttaacgaga gatatcatgc aacctaatat atttattaca ttaagtttat gggattctat
     1741 tgaatcgctc gataattatc gcaattctga attatttaaa acaacctggg cacaaaccaa
     1801 aattatgttt agcgaacgcc ccgatgcatg gtcgctggat aaactgatgt aaaatgcaaa
     1861 tttcaccgga taattatacc atttatattc aaccaacact tgatgattcc ggtgtattta
     1921 cttcatggtt aaatcagcac caatatagca atgtttttgt gttgtgtgat gataatacgt
     1981 taagatattg ttatccgcaa atcaaacaat ttttgccatc acatagttgt ataactattc
     2041 cagctggcga atcggaaaaa aatattgaaa catgtaaaaa tttgtggcaa caatttactg
     2101 atgcacaagc tgacagaaaa agtttattaa ttaaccttgg tggtggtgta attggtgata
     2161 tgggtggatt tgttgccagt acctataaac gcggtttcga ttttatcaat attccaacaa
     2221 cattattatc gcaggttgat gcgtctgttg gtggcaaact aggaattgat tttaatggtg
     2281 tgaaaaatat tattggatta tttcaaaacc ccaaagctgt ttttatatca ccagaatttt
     2341 taccgacatt accctttgaa caattacgca gtggttttag tgaaatttta aaacacggat
     2401 tgatttataa tgccgaatat tggcaccaac aaaaaggtaa aacagtggag ccaaattcag
     2461 attggcttgc tgcaattgaa caatcggttt taattaaaca tgaggttgta aaaaaagacc
     2521 cttttgaaag tggcttgcgc aaaattttaa attttggtca taccattggt catgctattg
     2581 aaacatggtc gctacaaaat gaaacgcaac cgctcttaca tggtgaagct attgccattg
     2641 gtatgatatg cgaagcctgg ttgtccaatg cagttaatgg gttgcctgct gatgagttgg
     2701 aagaaatcac ccatattttt ttgcagttat ttggaaaata tcctttggaa aaagttccac
     2761 ttgctgccat catggatgtg atgcaacagg ataagaaaaa ttccaataaa actattcggt
     2821 tcagtttatt acatgcaata ggtaattgtg attacgatat tcaggtaagc gatggcctaa
     2881 ttgccgatgc attaacttat tattgcacct tatcatgaaa tt
//
LOCUS       DAORKW010000477         2916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1952_length_2916_cov_5.046138, whole genome shotgun sequence.
ACCESSION   DAORKW010000477 DAORKW010000000
VERSION     DAORKW010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1952_length_2916_cov_5.046138"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLB46_14370"
     CDS             <1..465
                     /locus_tag="PLB46_14370"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HRF77758.1"
                     /translation="GILPLSVPLVHGREQRNTLGNVKISYEMAWQKNHWMTLTSAYRR
                     SAYYEYFEDMFAPLYHTHYVTLIEFNLAALTIVFKILGLKPEIRFTNNYIEPNTAGYA
                     DFRNMITPKNNTPLNYTFQPYNQVFSDRFPFMENLSIIDMIFNKGKFDLTEI"
     gene            472..1986
                     /locus_tag="PLB46_14375"
     CDS             472..1986
                     /locus_tag="PLB46_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF77759.1"
                     /translation="MKKIIIVFLLLNGLLLHAQQPAFITDSLDSYIQREMKRWQIPGL
                     AVAIVKGDKVVATKGYGVTDLDTKQAVDANTLFMIASNSKAFTGTSLALMEKNGQLNL
                     NDKVIDYLPYFAMHDPAITKMVTVEDVLSHRLGLETFQGDFVTWDSDLSRKQLIENFK
                     NNIPVYEFRDTYGYCNMGFLVAGEVIHAVTDTTWDDYLQYHFFNPLQMQRSSTTQAAL
                     LKDNNKCSPYTYFDGRIQKIDYDNVDNIGPAASINSSVNDLSHWLLMQVGNGNYKGKQ
                     IIPADVIQNTRTPRSIAGSGSSRLYKSQHFNLYGLGWFIKDLDGKKTLSHSGGANGFV
                     TNTVIIPEAQLGVVVLTNTDANGLYEALCYQIIEAYLNLPYRNLSEIYYSLYAPGFEE
                     AKEQMQNWEKLTGKNLSPALDLSAYTGTYFNPVYGKITITKAEKNLHVSFANHANLKG
                     VLKPAGDNLFICYYDPISWGVKEIPFFVEDGKVKSVTITINDFIDFLPYEFTKL"
     gene            1989..2564
                     /locus_tag="PLB46_14380"
     CDS             1989..2564
                     /locus_tag="PLB46_14380"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HRF77760.1"
                     /translation="MDEVYAAVIVPTNEIVETVKIFKQGLESVVGKFPAANADPGIIL
                     FAAMADETRAALLEKKLVTICRNQLPFTIQLNNFGEFYNNKTVFVDIDESSKQTITAF
                     RKKLSAEIKLSKYDELVDFNVGKTPHITIARGLVEDQYKVARKYFTGRNYTAAFECNC
                     LVWKKLIKTTSSTYYSPIRTFEFANKNLSLF"
     gene            complement(2602..>2916)
                     /locus_tag="PLB46_14385"
     CDS             complement(2602..>2916)
                     /locus_tag="PLB46_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRF77761.1"
                     /translation="DCSYQPGIVPIALKEVLPAFVAEHIREALLQIKKSKPGYFTNEA
                     VLVAPESRTSSPVKIPRNTETFEHIQIQGLYPCGEGAGYAGGIVSAAMDGMRIAVAIA
                     AK"
BASE COUNT          967 a          502 c          528 g          919 t
ORIGIN      
        1 ggcatattgc cattaagcgt acctctagtt catggtcgcg agcaacgcaa tacccttgga
       61 aatgttaaaa tcagttatga aatggcttgg caaaaaaatc actggatgac cctcacgagc
      121 gcgtatcgcc ggtctgctta ctacgaatat tttgaagaca tgtttgctcc gctttatcat
      181 acacattatg ttactttaat agaatttaat ttggcagcat taactattgt atttaaaata
      241 ttagggttaa aaccggaaat ccgttttaca aataattaca tcgaacccaa tacggcagga
      301 tatgctgact ttcggaatat gattacacca aaaaataaca caccactcaa ttatactttc
      361 caaccttata atcaggtttt ttcggaccgc ttccctttta tggaaaattt gagtattatt
      421 gatatgattt ttaataaagg taaatttgac ctaacagaaa tataaatact aatgaaaaaa
      481 attataatcg tttttcttct cctcaatggt ttgttgctcc atgcacaaca accggcattt
      541 attacggata gtctggacag ctatattcaa cgcgaaatga agcgctggca aataccgggt
      601 ttggctgtag ccattgttaa aggtgataaa gttgttgcta caaaaggata tggtgttacc
      661 gatttggata ctaaacaagc tgtagatgcc aatacattat ttatgattgc cagcaattca
      721 aaagcattca ccggaacttc actggcttta atggaaaaaa acgggcaatt gaatttaaat
      781 gacaaggtga ttgattattt accctatttt gctatgcacg atccggccat taccaaaatg
      841 gttactgttg aagatgtatt atcacatcgt ttgggtttgg aaacatttca aggtgatttc
      901 gttacttggg atagtgattt aagcaggaaa caattaatcg aaaactttaa aaataatatt
      961 ccggtttatg aattccgcga tacttacggg tattgcaata tgggattttt ggttgccgga
     1021 gaagtaattc acgcagtaac tgataccact tgggatgatt atttacaata tcattttttt
     1081 aacccgttgc aaatgcaacg cagcagcaca actcaagcag cattattaaa agataataac
     1141 aaatgttctc cttatacgta ttttgatggt cgtattcaaa aaattgatta cgacaatgta
     1201 gataatattg gacctgcggc aagtattaat tccagtgtga atgatttatc gcactggctg
     1261 ttaatgcaag taggtaatgg caattacaaa ggcaaacaaa taattccggc tgatgttatt
     1321 caaaacacgc gaacaccaag gtccatagca ggttcaggca gttcgcgatt atataagtcg
     1381 caacatttta atttatacgg attaggttgg tttataaaag atttagatgg taaaaaaacc
     1441 ttatctcaca gcggcggtgc aaacggattt gtaactaata cagtaattat tccggaagca
     1501 caattaggcg ttgttgtttt aacgaatacc gatgccaatg gattatatga agcgttgtgt
     1561 taccaaatta tcgaagccta tttaaattta ccttaccgca atttatcgga aatatattat
     1621 agtttatacg ctccgggttt tgaagaagca aaagaacaaa tgcaaaattg ggaaaaacta
     1681 accggtaaaa atctttcccc tgcattggac ttatcagcat ataccggtac gtattttaac
     1741 ccggtttatg gtaaaatcac cattacaaaa gctgagaaaa atttacatgt ttcttttgct
     1801 aatcatgcca atttaaaagg tgttttaaaa cctgcaggcg acaatctttt tatttgttat
     1861 tacgacccga taagctgggg agttaaagaa attccattct ttgttgaaga tggcaaagta
     1921 aaaagtgtaa ctattactat caacgatttt atcgatttct taccttatga atttacaaag
     1981 ttataggtat ggatgaggtg tatgcagcag taattgtccc cacaaatgaa attgtggaaa
     2041 cagtaaaaat tttcaaacag ggtttggagt ccgttgttgg caaattccct gctgcgaatg
     2101 ctgaccccgg cattatttta tttgctgcaa tggctgatga aacacgtgct gcattattag
     2161 aaaaaaaatt agtgacaatt tgtagaaatc aactgccatt taccatacaa cttaacaatt
     2221 ttggcgaatt ttataataac aaaactgttt ttgtagatat agatgaatca tcaaaacaaa
     2281 caataacagc atttcgaaaa aaattatctg ctgaaattaa attaagcaag tatgatgaat
     2341 tagttgattt taatgtaggt aaaacacctc atatcaccat tgcacgtggt ttagttgaag
     2401 atcaatacaa agttgccaga aaatatttta ccggacgcaa ttacaccgct gcttttgaat
     2461 gtaattgttt ggtctggaaa aaacttatta aaactacttc ttcgacttat tattcgccca
     2521 ttcggacttt tgaatttgcc aataaaaatt tgagtttatt ttaattgcct atacttaaaa
     2581 taggaagaat gaagtatcaa attatttagc tgcaatagcc actgcaatgc gcatgccatc
     2641 catagctgcg cttacaatcc cacctgcata accggcacct tcgccacaag gatataaacc
     2701 ctgaatttga atatgctcga atgtttcagt gtttcgtgga attttaaccg gtgatgatgt
     2761 tctcgattca ggtgcaacca aaactgcttc gttggtgaaa taaccgggtt tacttttttt
     2821 aatttgcaat aaagcttcac gaatatgttc agcgacaaat gcaggtaata cttcttttaa
     2881 tgctattgga acaattccgg gctgatagga acaatc
//
LOCUS       DAORKW010000478         2913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1956_length_2913_cov_4.747026, whole genome shotgun sequence.
ACCESSION   DAORKW010000478 DAORKW010000000
VERSION     DAORKW010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1956_length_2913_cov_4.747026"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PLB46_14390"
     CDS             complement(<1..763)
                     /locus_tag="PLB46_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRF77762.1"
                     /translation="MNLFEVINYLESIAPLSLQESYDNAGLITGNKQMEVTGILICLD
                     STEAVIDEAIASQCNVVIAHHPIIFGGLKKLTGSNYIEKTIIKAIKHDIAIYAIHTNL
                     DNIRAGVNAKIAEKLGLQHTKVLAPKGGLLKKLVTFAPEANAGDVRFALFEAGAGHIG
                     HYDHCSFSLEGTGTFRGDATTNPYVGTRGEDHFEAEVRIEVIYPAYLQSKLLTALIHA
                     HPYEEVAYDIYSLDNKFQEVGAGLIGTLPEPLDALT"
     gene            complement(797..1513)
                     /locus_tag="PLB46_14395"
     CDS             complement(797..1513)
                     /locus_tag="PLB46_14395"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRF77763.1"
                     /translation="MNGLLITAFVTGLFGSLHCAGMCGPLALSFNLHSKGNALVNGLT
                     YNISRIFAYVLLGFIFGAIGLSFSFAGWQQVASIVSGFVILLIIFFQQWSSAQPGFKW
                     LEKYNNYIKKKIGHSLSQTGVKGIATLGFFNGLLPCGLVYLALAGAVAGGGILIGGGY
                     MLLFGLGNLPLMFVFSAFGNYLGLQKKLLIRRYLPAFSVMIAVLLILRGLNLGIPYIS
                     PEFDTTPTLSASQPHVTCHK"
     gene            complement(1510..1941)
                     /locus_tag="PLB46_14400"
     CDS             complement(1510..1941)
                     /locus_tag="PLB46_14400"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRF77764.1"
                     /translation="MSWGKILLVAYVTFVGVMLFMVVKSFQQDYDLVTPDYYAAELKF
                     QDQIDASKNAAIYKDSIVIEPTDAAVNLQFPTAFRTSTSGQVYFYKASDSDRDVIQPL
                     ELDGSGKQQFNRTTFEKGFYTVKISWTKDNVQYYTEKELYL"
     gene            complement(1946..>2913)
                     /locus_tag="PLB46_14405"
     CDS             complement(1946..>2913)
                     /locus_tag="PLB46_14405"
                     /inference="COORDINATES: protein motif:HMM:NF023045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HRF77765.1"
                     /translation="WTGEKIFKKTAKQIIFFLFSFLVGNTFLSYIIGVDGVFEIATGS
                     FKENAGGFAIMMVFTFAFYGVFAFLREQVCTFACPYGRLQGVLLDKFSVIVAYDYKRG
                     EPRGHHKHGEEAKQLGDCIDCGLCVRVCPTGIDIRNGTQLECVNCTACIDACDSIMDK
                     IEKPRGLIRYTSENNIVTGEKLKFTPLMKAYSIVLFILVAVLTVMLVTRKDVTATVTR
                     ASGQLYQKRDSVYITNLYNIKIANKTHEGFPIHLKVENMDAKIEMIGKEVSIEKESLA
                     EGTFFIVMRRDQIKTMKTDLEIGVYNDEERIRTVKASFLGPVSKK"
BASE COUNT          926 a          589 c          489 g          909 t
ORIGIN      
        1 atgtgagggc atccagtggt tcaggcaagg ttccgattag cccagcgcca acttcctgaa
       61 atttgttgtc taaactgtat atgtcgtaag ctacctcttc ataagggtga gcatgtatta
      121 aggcggttag tagtttagac tgaaggtaag ccgggtaaat cacctcaatt ctgacttcgg
      181 cctcaaaatg gtcttctcct ctggttccaa catagggatt ggttgttgca tctccgcgga
      241 aagtgccagt accttccaag ctaaaactgc aatgatcata atggccaata tgacctgcgc
      301 cggcttcaaa taaggcaaaa cggacatctc cggcattggc ttcaggggca aaagtgacta
      361 atttttttag caatccgcct ttgggagcga gcactttggt atgctgtaaa cccaattttt
      421 cggcaatttt cgcatttact cctgcgcgaa tattatccaa attagtatgt atagcataaa
      481 tagcaatatc gtgtttgatg gctttgatga ttgttttttc gatgtaattg ctacctgtta
      541 gttttttaag tccgccgaaa atgattggat gatgcgcaat aacaacatta cattggctgg
      601 ctatcgcctc atctattacc gcttctgtag aatccagaca aattaatatg ccggttactt
      661 ccatctgctt atttccggtt attaaacccg cattgtcata actttcctgc aacgataatg
      721 gtgcaataga ttcgagataa ttaattactt caaacaaatt catacctgcg aaattaataa
      781 aactgtaggc taagtattac ttatgacaag taacatgtgg ctgagaagcc gataaagtag
      841 gtgtagtatc aaattcagga ctgatatatg ggattccgag atttaaacca cgaagtatta
      901 acagaacggc aatcatgaca ctaaatgccg gaaggtagcg tctgattaat aacttttttt
      961 gcaagcccag ataattacca aaagcagaaa aaacaaacat gagtggtaaa ttgcctaatc
     1021 caaacaatag catatatcca ccaccaatta aaataccacc acccgctact gcacctgcca
     1081 aagcaagata cactaaacca catggcaata atccattaaa aaatcctaaa gtggcaattc
     1141 cttttacacc tgtttgtgat aatgaatgcc cgattttctt ttttatgtag ttattatatt
     1201 tttcgagcca tttaaatccc ggttgagcag aggaccattg ttggaaaaaa ataataagta
     1261 agataacaaa ccctgaaaca attgaagcta cttgttgcca acctgcaaaa ctaaaactca
     1321 accctatggc accaaatata aagcccaata acacataggc gaatattctg gagatattat
     1381 aggttaaacc attaactaac gcatttcctt tgctatgtaa attaaatgat aaggccagtg
     1441 gtccgcacat acctgcgcag tgtaaactac cgaaaagtcc ggtaacaaaa gccgttatca
     1501 ataacccatt cacaaataga gttctttttc tgtataatat tgcacattat cttttgtcca
     1561 ggaaattttt acggtataaa atcctttttc aaaagtagtg cgattaaatt gttgttttcc
     1621 acttccgtct aattcgagtg gctgaataac atctctgtct gaatcagatg ctttataaaa
     1681 atacacctga ccgcttgtac tagttctgaa tgcggtagga aactgtaaat taaccgcagc
     1741 atctgttggt tcaattacaa tcgaatcttt atagatagca gcattttttg atgcatctat
     1801 ttggtcttgg aatttaagtt ctgcagcata ataatccggt gttaccaaat cataatcttg
     1861 ctgaaaactt tttactacca taaacaacat tacgccaaca aatgtcacgt aagctactaa
     1921 aagaattttt ccccagctca tagttttatt tttttgaaac aggtcctaaa aatgatgctt
     1981 ttactgttct gattcgttct tcatcgttat atacgccaat ctccaaatca gttttcattg
     2041 ttttaatttg atcacgtcgc attacaataa agaatgtacc ttcagctaat gattcttttt
     2101 caatactcac ttcttttcca atcatttcaa ttttagcgtc catgttttct actttcaaat
     2161 gaattggaaa tccttcgtgt gttttattgg caattttgat attatataaa ttggtaatat
     2221 acacactatc gcgtttttga tacaattgtc ctgaagctcg agtaactgta gcggtaacat
     2281 ctttacgtgt tacgagcata actgtcaata ctgccaccaa aataaacagc acaatagaat
     2341 atgctttcat aagtggagta aacttcagtt tttcaccggt aacaatattg ttttccgaag
     2401 tatatctgat gaggcctctt ggtttttcaa ttttatccat gatgctatca caagcatcta
     2461 tacaagctgt gcagttaaca cattctaatt gggtaccatt tctgatatcg ataccggttg
     2521 gacacacgcg aacacataaa ccacagtcga tacaatcgcc caattgttta gcttcttctc
     2581 cgtgtttatg gtgacctctt ggttcaccgc gtttataatc gtaagcaaca attactgaaa
     2641 atttatcgag taatacacct tgtaaacggc cataagggca agcgaatgta caaacctgct
     2701 cgcgcaaaaa tgcaaataca ccgtaaaatg caaaggtaaa taccatcata atggcaaaac
     2761 cacctgcatt ttctttaaac gaacctgttg ctatttcaaa tacgccgtca actccaatta
     2821 tataggataa aaatgtatta ccaactaaaa aagaaaacag aaagaaaata atttgttttg
     2881 cagttttttt gaaaatcttt tcaccggtcc atg
//
LOCUS       DAORKW010000479         2907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1965_length_2907_cov_4.136746, whole genome shotgun sequence.
ACCESSION   DAORKW010000479 DAORKW010000000
VERSION     DAORKW010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1965_length_2907_cov_4.136746"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..1120
                     /locus_tag="PLB46_14410"
     CDS             359..1120
                     /locus_tag="PLB46_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77766.1"
                     /translation="MKMSTIGWIGLAIGLIGGLAGLAVGIMAAPVEGSFFGGVFIGIF
                     FLIFWFFFRPMITANRILKTGEQRSGKILEVWDTNVTINNNPQVGLLVEVKDKYGKTY
                     QTKTKMVISRLQVGDVRPGQLVTVRVDPQNEQKIAIESFSATGDANYTKNEYEDDLQQ
                     ILGQIDKENKEILLSGISAEAKVVGYYDLNIKVNGDNPMVMLFLEIYPGLQTPFFAEA
                     KGVIAEQSVSKFQPGNMITVKYDPRNTSRVTVEHS"
     gene            complement(1242..2795)
                     /locus_tag="PLB46_14415"
     CDS             complement(1242..2795)
                     /locus_tag="PLB46_14415"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77767.1"
                     /translation="MKTLLTFTFLLLFYPFISLIAQSPSEFDPDFGTDGIVTTFDSGE
                     EESNAIIVQPDGKILIAVENNIRVSVFRFNTDGSVDTSFNHDGFTWTNVGAADIPQDI
                     ALQPDNKIVVAGISIPFGDLTEFGIVRYHPDGTLDSSFSGDGKLAFAFDADSDYGGAY
                     AVAIQPDGKIVLGGTVWGESGYDFAIARLNADGTFDESFDDDGKVVTDLDESSFDIIY
                     DIALQDDGKLVVVGVNMSAADSFVVVRYLPNGALDSEFDGDGKAFYDFGGLSGEVLND
                     VLIQPDGKILAAGAVNNGVTMNFALVRLLDDGSPDLDFNGTGYNITSFPEESYIKQIG
                     LLSDGKIVAAGKVYVDFDFQIAIASFTQDGILDNEFNGDGKLVFDYGEQDCFATALAI
                     QNDDKILATGSYFDGTDFAGFIARVFPVPAETLSVSNVNVPVFEIFPNPTDGNLSIVI
                     DNLENSSCLIELTDIYGKRIEIIEDAILAPGKNEIHFTFPEYLSTGNYLLHINSASSR
                     SVSLVSLVK"
BASE COUNT          956 a          560 c          513 g          878 t
ORIGIN      
        1 atatcataaa gcgatcatgt gagtggtcgc tttttttgtg tgggagattc tagttaaaag
       61 aaattgagca tatatgctgt ggatgaaaat tgttatggat tttaaaataa aaagcagatt
      121 tttcattggg tattagttaa atactaactt ttaagaattg aaaaataccg aaggctacct
      181 tcaaagaata aactccatgt gtgaaacagt atttcgtgat tttaatatgc aattaataaa
      241 ttaatcacct tttaaaatta aacataatta attatttgca aataatgtca ttataaccgc
      301 ttgttagcat tttatccatt ccccaaaatt ttttatcttt ccattaaaat cacatattat
      361 gaaaatgagc acaattggat ggatcgggtt ggccattgga ctaattggcg gacttgcagg
      421 gttggcagtg ggcattatgg ctgcacctgt ggagggttct ttttttggcg gtgtttttat
      481 tggtatattt tttcttattt tttggttttt tttccggcct atgattacgg caaaccgcat
      541 tttaaaaacc ggtgaacagc gcagcggaaa aatattggaa gtatgggaca cgaatgtcac
      601 cataaataac aacccacaag taggattatt ggtggaagtg aaagataaat acgggaaaac
      661 atatcagaca aaaaccaaaa tggttatttc ccgattacaa gttggcgatg tgcgtcccgg
      721 gcaattagta acggttcgtg tggatccaca aaatgaacaa aaaattgcaa ttgaatcgtt
      781 cagtgcaact ggtgatgcaa attatactaa aaatgaatat gaagacgacc tgcagcaaat
      841 tctcggtcaa atagataaag aaaataaaga aattttattg tccggtatat cagctgaagc
      901 aaaagttgtc ggttattacg accttaatat aaaagtgaat ggcgacaacc cgatggtaat
      961 gttatttctt gaaatttacc ccggcttaca aaccccattt tttgccgaag ccaaaggcgt
     1021 aattgccgag caatcggtat caaaatttca gcccggaaat atgattacag taaaatatga
     1081 ccctagaaat acttcgaggg tgacggtgga gcatagttga aataataaga attgattttt
     1141 ttcctgcaaa tagcagaacc gcaacttgca tttctaaaca ctccatagga gtgctagcca
     1201 agttagtgga aataactacg gcaaataaat tggtagttta tttattttac taaactcacc
     1261 aacgaaacac ttctactgga agctgaattg atatgcagca aataatttcc tgtgctcagg
     1321 tattcaggaa aggtaaaatg tatttcattt ttcccgggcg ctaaaattgc atcttcaatt
     1381 atttcaattc gtttgccata aatatccgtt aattcaatta aacaacttga attttcaaga
     1441 ttatcaatta caattgatag atttccatca gttggattag gaaaaatttc gaataccgga
     1501 acattaacat tggatactga aagcgtttct gcaggaaccg gaaaaactct tgcaataaac
     1561 cctgcaaaat cggtgccgtc aaaatagctt cctgttgcta atatcttgtc atcattttga
     1621 atagccaagg ctgtcgcgaa gcaatcctgc tctccatagt caaacaccaa tttaccatct
     1681 ccattaaatt cattgtccag aattccatcc tgtgtgaagg atgctattgc tatttgaaaa
     1741 tcaaaatcca cataaacttt accggcagca acaatttttc catccgacaa taatccaatt
     1801 tgcttgatat aactttcttc tggaaatgaa gttatattat atcccgttcc attaaaatcc
     1861 aggtccggtg acccatcatc cagcagtcga accagtgcaa agttcatagt tacgccatta
     1921 tttactgccc ctgcagcaag tattttacca tcaggttgta tcaatacatc atttaacact
     1981 tcaccactca aaccaccgaa gtcgtaaaaa gcttttccat caccatcaaa ttcagaatca
     2041 agtgctccgt taggtaaata ccttacaacc acaaaactat ctgccgcact catattaaca
     2101 ccaacaacta ctaattttcc atcatcctgt aaggcaatat catatattat gtcaaatgat
     2161 gattcatcga gatcggttac aaccttgcca tcatcatcga aactttcatc aaaagttcca
     2221 tctgcattta atctggctat tgcaaaatca tatccggatt caccccaaac agttccacct
     2281 aacactattt ttccatctgg ttgtattgct acagcataag caccgccata atcactgtcg
     2341 gcatcgaagg cgaaggcaag tttgccatca ccactgaaac tactatctaa agttccatca
     2401 ggatggtaac gtacaatacc aaattcagtc aggtcgccaa agggaatact tattccggca
     2461 acaacaattt tattatcggg ttgcaaggca atatcttgtg gtatgtctgc tgcaccaaca
     2521 ttagtccaag taaatccatc gtgattaaaa ctagtatcaa cagaaccatc agtgttaaat
     2581 cggaaaacgg aaacacgtat attattttct accgcaatta atatttttcc atcaggttgc
     2641 acaataatag catttgactc ctcctcgcca ctgtcaaatg tggtgactat accatcagta
     2701 ccgaaatcag ggtcaaattc gcttggtgat tgggcgatta agctaatgaa tgggtaaaag
     2761 agtaggagga aggtaaaagt gagcagcgtt ttcatttgta gatttttatc aaaattaata
     2821 aattttttat agcaataacg gcaggtgatt tgggtttcaa cggaggtaag gactgctacc
     2881 gctttcctcc ctaatgggtg cagttat
//
LOCUS       DAORKW010000480         2899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1973_length_2899_cov_5.415963, whole genome shotgun sequence.
ACCESSION   DAORKW010000480 DAORKW010000000
VERSION     DAORKW010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1973_length_2899_cov_5.415963"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1619)
                     /gene="rlmD"
                     /locus_tag="PLB46_14420"
     CDS             complement(210..1619)
                     /gene="rlmD"
                     /locus_tag="PLB46_14420"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRF77768.1"
                     /translation="MGRGKLRIREQVTIDTIADGGVGMGRVEGKVIFVEQVIPGDVVD
                     VQQTKKYKDYEMGYVTKFHSYSPLRVTPFCDHFQVCGGCTWQNVSYETQLDFKQRLVE
                     DAFRRIGKFTQLPAIEKITGSPYDRYYRNKLEFTFTNSGYINNPEFKKEDHANKKPAL
                     GFHVRKQFANVFDIQHCYLQPSLSNEIRLALKAYGLMHNLPFFDLRAKTGYLRNVIIR
                     NSSLGNLMVIIVVAADQPELLLPLMTHIKDTFPEITSLYYCVNEKNNDTIFDQDVVLF
                     SGEKYMIEQIDEVKYHLGPKSFFQTNFEQAKHLYHKALELAELKPDDQVYDFYTGLGS
                     IALLAAKYCKKVVGVELIEPAIEDAKMNATFNGIQNCTFIAGDMVKIFTDDFIAEHGR
                     ANVVITDPPRAGMHKDVCLKLLELEPEKIVYVSCNPVTQARDIQLLAEKYDVKLLQPV
                     DMFPQTYHVENIALLIKKS"
     gene            complement(1981..>2899)
                     /locus_tag="PLB46_14425"
     CDS             complement(1981..>2899)
                     /locus_tag="PLB46_14425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77769.1"
                     /translation="HVTGKLDDPLTEVTVDYSRANLSYGVPPQARQVPTHIFDTNDGR
                     VLGSYYENNQIQFVSNTLDPSTGFCGIYHGIITDLEGEKNIDAHIIGDDTLDIGYPNI
                     AYTGKFEGDNQSIITFDHTAPTVYAGMSAVFYNWGAYSDLVNIKTGDTYVNVLSGAYE
                     RWGDYTGSQRKYNEPGVIWVSGNFGKYIDGFPFIQRNNATWIASLRSTVEPNVAIPSV
                     TSNAKSTIYPNPADNLFYTKFTLEQSGVLAFYLYDMNGRLIKQLLTQQGQVGENIFSF
                     STDPLLPGNYILKITHNQLPFTTHSIIIK"
BASE COUNT          874 a          515 c          530 g          980 t
ORIGIN      
        1 tcctttaaca tgggatcgcg gtaatcaggc aatcagagtg tataaaacac ctgcgactgc
       61 ggccggaacc aatacattta atttaaatac ctggacaagt ggaacaggtg gaagctggtg
      121 gtatttctgg gcagaatcag gtacattttt tgaagctgct gaataatata atttagtctt
      181 aattaaaacg aaaatcctgg ctgtagtggt caggattttt ttatgagtag tgctatattt
      241 tctacgtggt aagtttgtgg aaacatatct acaggctgta ataatttaac gtcatatttt
      301 tcggctaata gttgaatatc acgcgcctgt gtaaccggat tacaacttac ataaacaatt
      361 ttttcaggtt ccagctctag taatttcaaa caaacgtctt tgtgcattcc tgcacgtggt
      421 gggtcggtga tgacaacatt tgcacgaccg tgttcagcaa taaaatcatc ggtaaatatt
      481 ttaaccatat cacctgcaat aaatgtacaa ttttgaatgc cattaaatgt tgcattcatt
      541 tttgcatctt caattgccgg ctcaattaat tcaacaccta ctactttttt acaatatttt
      601 gctgccagta atgcaatact tcccaatcct gtataaaaat catatacctg gtcgtcaggt
      661 tttaattcgg cgagctccag tgctttatga tataagtgtt tggcctgttc gaaattggtt
      721 tggaaaaaag attttggtcc gagatgatat ttaacctcat ctatttgttc aatcatatat
      781 ttttcgccgg aaaacaatac tacatcctgg tcgaaaatgg tatcgttgtt tttttcatta
      841 acacaataat acaaggatgt aatttcagga aaggtatcct ttatatgtgt cattaatggc
      901 aataataatt ccggttgatc agcggcaaca acaataatca ccatcaaatt accaagagaa
      961 gaattacgaa taattacatt acgcaaataa cctgtttttg ctctcaggtc gaaaaatggt
     1021 aaattgtgca tcaacccata agcctttaac gccaatcgaa tttcgttcga taacgaaggt
     1081 tgtaaataac aatgttgaat atcgaaaaca tttgcaaatt gtttgcgcac gtgaaatcct
     1141 aatgcaggtt ttttattcgc atggtcttct tttttaaatt cgggattatt gatatatcct
     1201 gaattagtaa aagtaaattc caatttattg cggtaatatc tatcgtaagg tgaaccggta
     1261 attttttcta tggccggtag ttgtgtaaat ttcccaatgc gacgaaatgc atcttctact
     1321 aaacgttgtt tgaaatctaa ttgggtttca taacttacat tttgccaggt gcatcctccg
     1381 catacttgaa agtgatcaca aaatggggtt acgcgtaatg gtgagtaact atgaaatttg
     1441 gtgacataac ccatttcata atctttgtat tttttagttt gttgcacatc aacaacatcg
     1501 ccgggaatta cttgttctac aaaaattact ttgccttcta cccttcccat tccaacaccg
     1561 ccatcagcaa ttgtgtcgat ggttacttgt tcgcgtattc ttaatttacc tctgcccatg
     1621 atttaattgc taaggtagtt ttttttaatg ggttggggaa ataaggtgta tgctgcctgg
     1681 ttttttttgc cgggaaggcg gggagatggg aagttacggg gggataaaat gttttttcta
     1741 tttttagttt aatgaaatcg cttttatttg aaaatgttgt tcggaaacaa gacttaaata
     1801 ttgccacaaa gaattaaagg ggccctaagt tactcaaagt ggtagatata tattcaataa
     1861 cagcattact gctacttact ttgcggtaat tataattctt gttattcttt acaacctttg
     1921 cgcttaattc ttagagttac ttcgagccgc ttagcgcctt cgtggtgaaa taataagcgt
     1981 ttatttgatg atgatggaat gggtggtgaa tggtaattga ttgtgggtta tttttaaaat
     2041 ataattacct ggtaaaagtg ggtcggttga aaatgaaaaa atgttttctc caacttgtcc
     2101 ctgctgagtg agtaattgtt taattaatcg tccattcata tcatataaat aaaatgccaa
     2161 cacgccactt tgctctaaag taaatttggt gtaaaataaa ttatctgcag ggttgggata
     2221 tattgttgat ttcgcatttg atgtaactga tggaattgca acattgggtt caacagtact
     2281 tcttaatgat gcaatccatg tggcattatt gcgttgaata aatggaaatc catcgatata
     2341 ttttccgaaa ttaccactca cccaaatcac accaggttca ttgtatttac gttgtgagcc
     2401 ggtgtaatcg ccccaacgtt catatgcacc ggacaataca tttacatatg tatcgccggt
     2461 tttaatattt actaaatcgg aataggcacc ccagttataa aacaccgcac tcatacctgc
     2521 atatactgtt ggagcagtat gatcaaaagt aataatactc tgattgtcgc cttcaaattt
     2581 tccggtataa gcaatattcg gataaccaat atctaaagta tcatcgccaa taatatgcgc
     2641 atcaatattt ttttcacctt ccaaatcggt tataattcca tgataaattc cacaaaatcc
     2701 tgttgacgga tcgagtgtat ttgaaacaaa ttgtatctga ttattttcat aataacttcc
     2761 taatactctg ccatcattgg tatcaaaaat atgggtaggc acttgtcttg cctgtggagg
     2821 cacaccgtac gataaattag cacgtgaata atcaaccgta acttccgtta acgggtcatc
     2881 caatttacca gttacatgc
//
LOCUS       DAORKW010000481         2894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1980_length_2894_cov_4.113420, whole genome shotgun sequence.
ACCESSION   DAORKW010000481 DAORKW010000000
VERSION     DAORKW010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1980_length_2894_cov_4.113420"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLB46_14430"
     CDS             complement(<1..483)
                     /locus_tag="PLB46_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77770.1"
                     /translation="MYKLVFNNEQFDEQKLRYLMTDLTKHLEEFLCYKAISEEQIFKY
                     HLLLYTYRLRRLDKAFLNTLKLAEKALNDQPRRDVSHAFYQYLIEEDRYRFMSDRREH
                     LHETNLQQVVDNLDKYYILNKMRYSAEIINNKNVVAINYKLFLYDEIMNHIRHNPLDY
                     V"
     gene            935..1444
                     /locus_tag="PLB46_14435"
     CDS             935..1444
                     /locus_tag="PLB46_14435"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77771.1"
                     /translation="MKKLFFTLLLVSVASAMFAQSNARVRSEDTNNPEIDNLSGLKPD
                     ETDLIPSSGVANFSTDNTATGTTGTSDDSFNTKGITNENMLNGKNTETLETTQVYPNP
                     AKEFVTVAVEVESGTIRILNLLGQEMGVYNINSNLTSIDVSNFGEGIYFVSIESGAMK
                     ITKKIKILY"
     gene            1757..1951
                     /locus_tag="PLB46_14440"
     CDS             1757..1951
                     /locus_tag="PLB46_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77772.1"
                     /translation="MAHVGITIATEESYIFSAAEYQMSEQPAATTNNLVALPTAGVQS
                     QPSITSPAGMFSTEDAFFTF"
     gene            2024..2647
                     /locus_tag="PLB46_14445"
     CDS             2024..2647
                     /locus_tag="PLB46_14445"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF77773.1"
                     /translation="MRYFYLLLVIIFAQIGKAQTTDTVIQFSGIVMTTDSLMAIPYAN
                     VLASNSKTATTSNYNGFFSFVAAKGDTILFTAVGYKDAKYIIPGDLTENKYSVIQLMT
                     RDTIYLTETIIYPWPSKEEFRDAFLAYDIPDDYYETARKNLERERLKEIGEAMAMDAD
                     MNADYQTKVLGQKIYYAGQYPPMRIFDVFAWKQFFEAWQNGDFKKED"
BASE COUNT          921 a          545 c          517 g          911 t
ORIGIN      
        1 aacataatcc aatgggttat gcctgatatg attcataatt tcatcataca ggaacaactt
       61 ataattgata gcaactacat ttttattatt gataatttcg gcactatagc gcattttatt
      121 caggatataa tatttatcca gattatctac cacctgttgc aaattggttt cgtgcaaatg
      181 ctcacgcctg tcgctcataa accgataacg gtcctcttca attaaatatt gataaaacgc
      241 gtgactgaca tctctgcgcg gttgatcgtt taatgctttt tctgcaagct ttaaggtgtt
      301 aagaaatgct ttatccaaac gtcgcagccg ataagtatat aataacaaat gatatttaaa
      361 aatctgctcc tccgaaatcg ctttgtagca taaaaattct tccagatgtt tggttaggtc
      421 ggtcatcaaa tatcgcaact tctgttcgtc aaactgttcg ttattaaaaa ccagtttata
      481 caattgcgtt cgctctaaat ttggatgttc aaataagggg aaccaagcct ttattatatc
      541 aaaggcctga attatggttt gatttttgtt gaagaagggt gaacggaggt actcgtcaaa
      601 ttgacgcacc tcctgagagg tgaaagttct taatatgacg atggacttat tttgaaacat
      661 tgattaaaat cggttaaatt taacaaagga tttgttgtaa acagcaaatc aacaactttt
      721 caaaccgata gaattgtcat ttttcaaggc cctaaaatta ctaaactgtt gataatcagt
      781 gaaatacata ttaacattta gttaaaaact ttttaagtgt gccgataaag agttatattt
      841 gtcttaaaga agtgtgaaga ggtgtcttta tttttaaaac taattacagg cgaaacccgt
      901 ataagaacta ttaattgacc ttaatattac tgctatgaag aagttatttt ttaccctttt
      961 actagtgtcc gttgcaagtg caatgtttgc acaaagtaat gcgagagtta gatcagagga
     1021 taccaacaac cctgaaatag acaatctcag cggtttaaaa cctgatgaaa ctgacttaat
     1081 accaagttca ggagtagcta atttctcaac tgataataca gccactggaa ctacaggaac
     1141 ttcagatgat agctttaata ccaaaggcat tactaacgaa aatatgctca atggtaaaaa
     1201 caccgaaaca ttagaaacta cacaggttta tccaaaccct gctaaagagt ttgtaaccgt
     1261 tgctgtggaa gtagaaagcg gaaccattcg tatattaaat ctgttaggtc aggaaatggg
     1321 tgtttacaac atcaattcga accttaccag cattgatgta agcaattttg gcgaaggcat
     1381 atacttcgtg tcaattgaat caggtgcaat gaaaattact aaaaaaataa agattttata
     1441 ttgatttttt aggggttaaa tcaaacaaaa gcctctccga cagccggagg ggttttttgt
     1501 ttttagacat gtccgctcat ataccccgta ccgataagcc ctcatttttg tcatgatttt
     1561 ttcatttaac attttattaa tttcaatgaa atcaagggtt ttagccgaaa accgctgttt
     1621 ttaagtgtta aaatgtgatt gataacccta ccgattttgt acttggcatg ggccgataac
     1681 cgaagtaatt tggcatcata aatcaatgac agctatgaaa aagttaaccg gaaccatcgt
     1741 acttgccgca gtgttaatgg cacatgtagg cataaccata gccactgaag agtcttatat
     1801 cttctctgca gccgaatacc agatgtctga acagccagct gctaccacga ataatcttgt
     1861 agcacttcca acagccggcg tgcaatcaca accttctatc acaagccctg caggtatgtt
     1921 ttctaccgaa gatgctttct ttacgttcta attgattaat tcccactcat ccgtcgttcg
     1981 gttcaggaca ataagcgtat ctttgaatta cgttaattca acaatgcgct atttttactt
     2041 attacttgtt atcatttttg ctcaaattgg caaggcacaa actaccgata ctgtaattca
     2101 gttttcggga attgtgatga ctaccgatag tttgatggca ataccctatg ccaacgtatt
     2161 agcaagtaac agtaaaacgg caactaccag caattacaat ggtttctttt cgtttgtcgc
     2221 agctaaaggc gataccattt tatttactgc ggttggttat aaggatgcaa aatatattat
     2281 tcccggcgat ttaactgaaa acaaatattc agttattcaa ttaatgaccc gtgatactat
     2341 ttatttaaca gaaacgatta tttatccctg gccatcaaaa gaagaattca gagatgcatt
     2401 tttagcctac gatattccgg atgattatta tgaaaccgct cgcaaaaatc ttgaacgaga
     2461 acgattaaaa gaaattggcg aggcaatggc catggacgcc gatatgaatg ccgattatca
     2521 aacaaaagtg ctcggtcaaa aaatttatta cgccgggcaa tacccgccaa tgcgcatttt
     2581 tgatgtgttt gcttggaaac aattttttga agcctggcaa aacggggatt ttaaaaaaga
     2641 agactaaatt tttttacgcg gaaacgcgga ggcaattttt ttatataatg tggtttgttt
     2701 tgagcgcgcg gagcttacaa accaacccaa tctccgcctt ctccctcctc cgcgctttca
     2761 gtccgctgag gcgggtgaaa caatagtgga atttctccca accttacttc gcagaaaact
     2821 tcattttata ctttgcagct atatttcctt tagcaatatt atccagtttc tttttaatta
     2881 aactgcgttt taag
//
LOCUS       DAORKW010000482         2888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1985_length_2888_cov_4.227674, whole genome shotgun sequence.
ACCESSION   DAORKW010000482 DAORKW010000000
VERSION     DAORKW010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1985_length_2888_cov_4.227674"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PLB46_14450"
     CDS             <1..869
                     /locus_tag="PLB46_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77774.1"
                     /translation="LQPLAPSITDITHAEKGSTRNITNNDPSTRGLRIYRKLKTDSLY
                     QLVSELVKISGTNIVFTDTTGLQSGGYYTYAVKAENTAYILSKNSNEVSIRLTTTKSI
                     PEMGIPSVTQVSKGLLVVWKPLSNTAITGFKLYRKNELGEVICLEQNAPQQQLAYLDT
                     VLVTGKYSYGIQYFDDMGNGSAIAYSDAVVYAGNRLSTKGPANVIVNKLNEQTIISWK
                     PINENNIKGYRIYRITGDTTTNLLGEVSATNNQFTLQNTKPEEVYRIFITYVTEGNLE
                     SEPGKIVNYIVE"
     gene            complement(875..1840)
                     /locus_tag="PLB46_14455"
     CDS             complement(875..1840)
                     /locus_tag="PLB46_14455"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRF77775.1"
                     /translation="MKAIIHTRYGAPDVLQLAEVPKPVPASDEVLVKIFATTVNRTDC
                     GFRKPEYVFVYLISGLFKPKNKILGTEFAGVVEAVGTGVTKFNIGDNVFGLRTYKFGT
                     HAEYVCVKQHGSIATKPHNMNYQEAAAVCDGLMLAINYIRKIDFSKPVKLLINGASGS
                     IGTAAVQLAKYYGAQIHAVANTKNIELIKSLGAEKVFDYTREDFTKQNEQYDVILDAV
                     GKSSYFKCKHLLKSGGVYFSTELGYAMQNVWLPLLNVFKNKKVKFPIPTDNQQDILFF
                     KKIIEEGKYRAIIERVYPLHEIVEATKYVETGQKTGNIVIQVNEG"
     gene            complement(1869..2201)
                     /locus_tag="PLB46_14460"
     CDS             complement(1869..2201)
                     /locus_tag="PLB46_14460"
                     /inference="COORDINATES: protein motif:HMM:NF040156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5713 family protein"
                     /protein_id="HRF77776.1"
                     /translation="MTPNKFPEDFEYLKEMYDDAYFPNFLVDKIKEIIQEVVTFLESG
                     NHSMTQIQDAFDAMTLKINAMQNEFDEHNSELETVARESIADTVDQILSHFDIDLDIE
                     EAIRVREW"
     gene            complement(2356..>2888)
                     /locus_tag="PLB46_14465"
     CDS             complement(2356..>2888)
                     /locus_tag="PLB46_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF77777.1"
                     /translation="KEKRQNVYNDFHAVAEDLIKRKITAPEHLGIMGGSNGGLLMGVA
                     FTQRPDLYKAVVCQVPLLDMERYNKLLAGASWMGEYGNPDIPEEWEFIKKYSPYHNLK
                     ENAAYPEVFFTTSTRDDRVHPGHARKMVAKMESMGYKVYYYENIEGGHAGASTNEQRA
                     KSAALEYSYLWMKLKH"
BASE COUNT          905 a          541 c          516 g          926 t
ORIGIN      
        1 cattacaacc acttgcgcca agtattaccg atattactca tgctgaaaaa ggtagtacac
       61 gtaacattac taataacgat ccgtccacaa gaggattgag aatttatcgc aaattaaaaa
      121 cagattcttt gtatcagttg gtgtctgagt tagtaaaaat cagtggaaca aatatcgttt
      181 ttaccgatac tacaggatta caaagtggag gttattacac ctatgccgtt aaagcggaaa
      241 acactgctta tatacttagt aaaaacagta acgaagtgtc tattagatta accactacaa
      301 aaagtattcc ggaaatgggt attccgtctg taacccaggt atctaaaggg ctgctggttg
      361 tttggaaacc actatccaat actgccatta ccggttttaa actttataga aaaaacgaat
      421 tgggagaggt aatttgtctc gaacaaaatg cgccgcaaca acaattagct tatttagata
      481 cggtattagt gaccggtaaa tatagttatg gcattcaata ttttgacgat atgggaaatg
      541 gttctgcaat tgcttacagt gatgccgttg tatatgccgg taatcgcttg agcacaaaag
      601 gccccgctaa tgtgattgta aataaactaa acgaacaaac gattataagc tggaaaccta
      661 ttaatgaaaa taatattaaa ggttatcgca tttatcgtat aaccggagat acaaccacaa
      721 atttattggg agaagttagt gcaacaaaca atcagtttac tttacaaaat accaaacctg
      781 aagaagtata tcgcatcttc attacttatg ttacagaagg aaatttagaa agtgagccgg
      841 gaaaaatcgt caattatatt gttgaataaa tttatcaacc ttcattaacc tgaataacaa
      901 tattgccggt tttttgaccg gtttcgacat attttgttgc ttcaacaatt tcatggaggg
      961 gataaacacg ttcaataatt gcgcggtatt ttccttcttc aataattttt ttgaagaata
     1021 aaatatcctg ttggttatcg gttggtattg ggaattttac ttttttattt ttgaaaacat
     1081 tcaataacgg cagccacacg ttttgcatcg cataacccaa ttctgtagaa aaatatacgc
     1141 caccggattt tagtaaatgt ttacatttaa aataagagct tttgcccact gcgtctaaaa
     1201 taacatcata ttgttcgttt tgtttggtaa aatcttcccg ggtataatcg aacacttttt
     1261 ctgcaccgag ggattttatc aattcaatat ttttggtatt ggcgacggca tgaatttgtg
     1321 caccataata tttagctaat tgcactgcag cagtaccaat agaacctgac gcgccgttaa
     1381 ttaatagttt aaccggttta ctgaaatcaa ttttacgaat ataatttatg gctaacatga
     1441 gtccatcgca taccgctgca gcttcctgat aattcatgtt atgtggtttg gttgcgattg
     1501 aaccatgttg ttttacacaa acatattctg catgggtgcc gaatttataa gttctaagtc
     1561 caaaaacgtt atcgccaata ttaaattttg tcacaccggt tcctactgct tctactaccc
     1621 cggcgaattc agtgcctaaa attttatttt tgggtttaaa caatccggag attaaataca
     1681 caaaaacata ttcaggctta cgaaaaccac aatcggttct gtttacagta gttgcgaaaa
     1741 ttttcaccaa aacttcgtca ctagccggca ccggtttagg cacttctgcg agttgtaata
     1801 catcaggtgc accgtatcgc gtatgtataa ttgctttcat tgttgtgata aagatacatt
     1861 ttgcatattt accattctct tacacgaatg gcttcttcaa tatcgaggtc gatatcgaaa
     1921 tgcgataaaa tttgatcaac ggtgtcggca attgattcac gggcgacagt ttccaattcg
     1981 ctgttgtgtt catcaaattc attttgcatg gcatttattt ttaatgtcat cgcatcaaac
     2041 gcatcttgaa tttgtgtcat actgtgatta cctgactcca aaaaggtgac aacctcctga
     2101 ataatttctt taatcttatc cactaaaaaa ttagggaaat aagcatcgtc atacatttct
     2161 ttaaggtatt caaaatcctc gggaaatttg tttggtgtca tagtgaatga aagttagggt
     2221 attattgaac gtatatcaaa agtatacaat atttattaaa ctgcggctgc agcctgttta
     2281 acaaacaaaa gcgctgtggg acaaggccaa cagcgctttt ataacttgca attgaatgta
     2341 accgaaaggt gaagcttaat gtttcagttt catccacaaa taagaatatt caagggcagc
     2401 agattttgca cgttgttcat tggtagaagc accggcatgt ccgccttcaa tgttttcgta
     2461 gtaatatact ttatacccca tagactccat ttttgcgacc attttacgcg catggccggg
     2521 atgcaccctg tcgtcgcggg tagaagttgt aaaaaacact tcaggataag ctgcattttc
     2581 tttgaggtta tgatatgggg aatatttttt aataaattcc cattcttccg gaatatccgg
     2641 attgccatat tcgcccatcc aacttgcgcc tgctaataat ttattatatc gttccatatc
     2701 gagcagtggc acctgacaaa ctacagcctt atataaatca ggacgttgcg taaatgccac
     2761 acccattaat aaaccgccat tacttccacc cattatacca agatgttcgg gagcagtaat
     2821 ttttctttta attaaatctt cagcaacagc gtgaaagtcg ttatatacat tttgacgttt
     2881 ttctttta
//
LOCUS       DAORKW010000483         2875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_1997_length_2875_cov_6.076596, whole genome shotgun sequence.
ACCESSION   DAORKW010000483 DAORKW010000000
VERSION     DAORKW010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1997_length_2875_cov_6.076596"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /gene="mnmE"
                     /locus_tag="PLB46_14470"
     CDS             complement(<1..995)
                     /gene="mnmE"
                     /locus_tag="PLB46_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRF77778.1"
                     /translation="MAEHKDTIVALSTAPGVGAIAVIRMSGDNAIEIADKVFIGKKLA
                     RAQSHTLHFGVIAVEGNKIDEVVIGLFKNPRSYTGEDIIEISCHGSPFIQQQILQVLI
                     QAGARLAKPGEFTMRAFLNGKMDLSQAEAVADLIAAQTQASHDIALKQMRSGYGETLK
                     QLRFELIHFASLIELELDFSEEDVEFAERSHMQHLIDKINLELDRLIRSFELGNVIKN
                     GVNTVIAGRPNAGKSTLLNALLNEDRAIVSAIAGTTRDTIEEELNINGIVFRLIDTAG
                     IREAKDEIEKIGVEKTMEKIKTSTLLMYVFDVNTLTPEEVRTDLNNLVEAGDRMLV"
     gene            1043..1831
                     /locus_tag="PLB46_14475"
     CDS             1043..1831
                     /locus_tag="PLB46_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77779.1"
                     /translation="MSNEKQRSAFTTVVGIVLTAVGALITFVGGSALLSTTWDFIGIL
                     VLLLTLAGLLLLVSGISQLFIARDVKEKQHQIMESLHTKPAEGDVHTSISNQPVRAHW
                     SIDEATWKLFAKNEIKYRNEDNIYFSIAFFALGTITIMLSRSASLPIAIIITLIIGSI
                     IVYIRRKLALAKLKPGKGKKEVYITDYFVIMNGEQYYLMNEHRLTSKVILYEDTSPAI
                     LEFTIHWQTRNGVTFDELRLPVPPEAIPLAKEISLNFKTVNLPR"
     gene            1828..2619
                     /locus_tag="PLB46_14480"
     CDS             1828..2619
                     /locus_tag="PLB46_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77780.1"
                     /translation="MKNSIVLIILLLLVASVNAQFLPGDVGFNANEYTLYKKNKVNAV
                     KITVYDSEGYLGELQTNYYDTEGKLQKVVYDMASDFEDSEQESPMNDVFIYNKESNVS
                     QINMNGYDMMEIQVGFEYKKGKLEKSFVSGAEAREYSYVYDKSEHIIEKIGNAMSFEV
                     DDEGNSTDKMVMTEVEKSVYTWDKNGNLIDETFYMYGEFFFKTIYNYNKEGMLVSYNR
                     YYDPTNLSTPSFKMVFEFNDKGLPIKSVAEEDGIMLINQYTYTFY"
     gene            2694..>2875
                     /locus_tag="PLB46_14485"
     CDS             2694..>2875
                     /locus_tag="PLB46_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HRF77781.1"
                     /translation="MKILMVCLGNICRSPLAEGILRHKAKQNGLSWHVESAGTGAWHV
                     GQAPDPRSQKIAKLHG"
BASE COUNT          900 a          512 c          526 g          937 t
ORIGIN      
        1 accaacatgc ggtcacctgc ttccactaaa ttatttaaat cggtgcgaac ttcttcgggc
       61 gtcaaggtat ttacatcaaa tacatacatc aacaatgtag atgtttttat tttctccatt
      121 gttttttcta cgccaatttt ttctatttca tctttggctt cgcggatacc ggctgtatca
      181 attaatcgaa atacgatgcc attaatattt aattcttctt caatcgtatc gcgggtggtt
      241 cctgctattg cacttactat tgctctgtct tcatttaata atgcatttaa caaagtagat
      301 tttccggcat ttggccttcc tgcaattacc gtatttacac cgtttttaat cacattacct
      361 aattcaaatg aacggattaa tcggtctaat tctaaattaa ttttatcaat taaatgttgc
      421 atatgactgc gctctgcaaa ctcaacatct tcttcgctaa aatctaattc caattctatt
      481 aatgatgcaa aatgaattaa ttcaaaacgc aattgtttta atgtttcgcc atatccgctg
      541 cgcatttgtt ttaatgcgat atcatgcgag gcttgtgttt gcgccgcaat taaatcggca
      601 acagcctcgg cctgacttaa atccatttta ccatttaaaa atgcacgcat ggtaaattct
      661 ccgggttttg caagtcgcgc accggcttga attaatactt gtaaaatttg ctgttgaata
      721 aatggagaac catgacagga aatttctatg atgtcttcac ctgtatatga acgtggattt
      781 ttaaataatc caattaccac ttcatcaatt ttatttccct ccacagcaat aacaccaaaa
      841 tgtaaagtat gggactgggc acgtgcaagt tttttaccta taaatacttt gtccgcaatt
      901 tcaatggcat tatcgcccga cattctgata acagcaattg caccaacacc aggtgcagtg
      961 cttaaggcaa ctattgtatc tttgtgttca gccaattgca tattttgcga aggtaagacc
     1021 ttaagttcaa aaaaacactg aaatgagtaa tgaaaaacag cggtccgcgt ttacaacggt
     1081 agttggaata gtacttactg ccgttggtgc actcattacc tttgttggag gctctgcttt
     1141 actcagtacc acatgggatt ttattgggat attggtgttg ttgcttaccc tagccggttt
     1201 actattgttg gtgagcggta tcagtcagct attcattgcc cgtgatgtaa aagaaaaaca
     1261 acatcaaatc atggaaagcc ttcataccaa accagccgaa ggtgatgttc atacatcaat
     1321 cagcaaccag ccggttcgtg ctcattggtc aattgatgaa gctacatgga aattgtttgc
     1381 gaaaaatgag attaaatatc gcaacgaaga caatatttat ttttccattg ccttttttgc
     1441 tttaggcacc ataacaatca tgttatcgcg tagtgccagt ttgcctattg ctattataat
     1501 aacattaatt attggaagca ttattgttta tatccgccgc aaattagcac tggcaaaatt
     1561 aaaaccaggt aagggtaaaa aagaagtgta tattactgat tactttgtaa tcatgaatgg
     1621 cgaacaatat tatttaatga atgaacatcg tttaactagt aaagtaatat tgtatgagga
     1681 tacatcgccg gcaattttag aattcactat tcactggcaa acacgcaatg gtgtaacatt
     1741 tgacgaactt cggttaccgg ttccgcctga agcaatacct ttagctaaag aaatttctct
     1801 taattttaaa actgtaaatc taccacgatg aaaaatagta ttgtattaat tattttgttg
     1861 ctgctggttg catctgtaaa tgcacaattt ttgcctggtg atgtaggctt caatgcaaat
     1921 gaatatacct tatataaaaa gaataaagta aatgctgtaa aaattactgt ttatgattca
     1981 gaaggttatc tcggtgaatt acaaactaat tattatgata ctgaaggtaa attacaaaag
     2041 gtagtttatg acatggcttc agattttgaa gatagcgaac aagaatcgcc tatgaatgat
     2101 gtttttattt ataataaaga atccaacgtt tcacaaatca atatgaacgg ttatgatatg
     2161 atggaaatac aagttggatt tgagtataaa aaagggaaac tggaaaaaag ttttgtttcc
     2221 ggtgcagaag cacgtgaata cagttacgtt tacgataaat cagaacatat tatcgaaaag
     2281 attggtaatg cgatgagctt tgaagttgat gacgagggca acagtaccga taaaatggtt
     2341 atgactgaag tagaaaagag tgtttacacc tgggataaaa atggcaattt aattgatgag
     2401 acattttata tgtacggtga attctttttt aaaaccatat acaactacaa taaagaaggc
     2461 atgttggtta gttacaacag atattacgac cctacaaatt tatctacccc ttcttttaaa
     2521 atggtttttg agtttaacga taaagggttg ccaattaaat cggttgccga agaggacggt
     2581 atcatgctga ttaaccaata tacctatacc ttttattgat actagcttta gtggtatgtt
     2641 aatttgtaaa taccttgcaa cttttactgc ctgtctttgt ttaactttgc agtatgaaaa
     2701 ttttaatggt ttgcttgggc aacatttgtc gcagtccttt ggctgagggc attttgcgcc
     2761 ataaagcaaa acaaaatggg ctttcatggc atgtcgaaag cgccggcaca ggtgcatggc
     2821 atgttgggca ggctcccgac cccaggtccc aaaaaattgc caaattgcat ggtat
//
LOCUS       DAORKW010000484         2861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2008_length_2861_cov_3.983250, whole genome shotgun sequence.
ACCESSION   DAORKW010000484 DAORKW010000000
VERSION     DAORKW010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2008_length_2861_cov_3.983250"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2773)
                     /locus_tag="PLB46_14490"
     CDS             complement(<1..2773)
                     /locus_tag="PLB46_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77782.1"
                     /translation="MPSNSNLDSALSCLSTDSVNTQLTDDAIFNVVLDQGNYMLSKTL
                     TLNQEALDFYAQMYVDTASCLLTLEDFIDSAMADLDTTGCYLDCDYCDSIRDLSVEEY
                     ISANMDSAGSSADTLMWKEMFYSNIQQCEILCEIGVGNDPCFAGYMMMLQDMSPGGQY
                     AELYNLATGEIDVDYFALSVLNESNILQVRTVDAIEYEPNWRNPKLKNNAGDWISGYY
                     DENGTTRAIIYPVDTGLGYYPESIDGIGHYTADGIRFIYPEEIKYVQDFVSLFQPSWA
                     NSLIIYHPEYFYYQFCAEDSYDCFAIVGSDTLNPFQFDEYLLSYSRDSIFNIDTNLIH
                     IDEVDPYVVDDCGTGHTDLRLEVEYFETCTDATIIETANLTLWETIWMTNHLDFTSSW
                     TCEEIQDTLNDQFNLTSPPSLWNYIQTDKDWNNLVSLYAAERWNYIFRRATIVSNALK
                     AGNQCIGEGAAFYDTDGDGILDMGIGDLFDGDICDWPSGIYYYDKIKRFQTPQTIYGI
                     SESELNDDDILVGIIDSLEAEGETAYFEYTGTLPLASDLQWFLQGWVIHTDGICATNP
                     LNENPVLTIDLFNALDDAGFDDWEPQLSTDSLVLKWSSEIDTSCKLVLNFPANIENHN
                     WNTSIQNIMNIHVLDADIGTFSVQVVVTDEESGELLNYEATGSICTEIILNDSSILLS
                     NCEPTIEASAMMLIMNNLLIYDNFNDASYTLTAAEYEPLVPILEPYIGTNVPFKWEHP
                     AATNDYYLKNADESNEIKISFNSTIPYTIGDTSFFNDLNGISNPAFVGTVNNFSAIVY
                     TDFPAVSPVTSVTGTMYWLNEATYEAFPVGDCDFPISTPCSDDEHYLLNDLQTLLNYV
                     VVNNFVETGDSLILDDLEEAGYSPLFAEYSGGSAATDYWYVDHVSDNTIEASLKNISG
                     GQIESNCQVY"
BASE COUNT          929 a          588 c          451 g          893 t
ORIGIN      
        1 aataaacttg gcaattagat tcaatttgtc ctccggaaat attttttagt gatgcttcaa
       61 tcgtattatc tgaaacatgg tcaacatacc agtaatctgt tgctgcagaa ccaccactgt
      121 attcagcaaa aagaggcgaa tatccggctt cttccaaatc gtccagaatt aatgagtcac
      181 ctgtttctac aaagttgttc acaaccacat aattgagtaa agtttgcaaa tcgttgagaa
      241 gataatgttc atcatcgcta catggcgttg aaatcggaaa atcacaatca cctacaggaa
      301 atgcctcgta tgttgcctca tttaaccaat acattgttcc agtaactgaa gttacgggac
      361 taactgctgg gaaatcagta tatacaatag ctgaaaaatt attaacagtt ccaacaaacg
      421 ccgggtttga gattccgttt aaatcattaa aaaacgatgt atctccaata gtgtatggta
      481 tagtgctatt aaacgaaatt ttaatttcat ttgattcgtc tgcatttttc aaataataat
      541 cgtttgttgc agctgggtgt tcccatttaa atggcacgtt agttccgata tatggttcta
      601 gaattggaac caatggctca tattctgcag cagttagggt atatgatgca tcattaaaat
      661 tatcataaat caacaaattg ttcattatta acatcattgc actagcctca attgttggtt
      721 cacaattact aagcaaaata gaactatcat ttaaaataat ctcagtgcaa atagaaccgg
      781 ttgcttcata attgagcaat tcgccactct cttcatcagt cacaaccact tgcacactaa
      841 acgtcccaat atcggcatct agtacatgaa tattcatgat attttgaata gatgtattcc
      901 aattgtggtt ttcaatattc gcaggaaaat tgaggaccaa tttacatgaa gtatcaattt
      961 ctgatgacca ttttagcacc aacgaatcag ttgataattg aggctcccaa tcatcaaacc
     1021 ctgcatcatc aagtgcatta aataaatcta tagttaatac cgggttttcg ttgagcggat
     1081 ttgttgcaca aattccatct gtatgaatta cccaaccttg taaaaaccat tgtaaatcac
     1141 ttgctaatgg caatgttccg gtgtattcaa agtaagcagt ttcaccttca gcctccaaag
     1201 agtcaataat tccgactaaa atatcatcat cattaagttc actttccgat atgccgtaaa
     1261 tagtttgcgg agtttgaaat cgcttgattt tatcataata gtaaattcca ctcggccaat
     1321 cacaaatgtc tccgtcaaat aaatctccga tgcccatatc taaaattcca tcaccatctg
     1381 tatcataaaa agctgcccct tcaccaatac attgatttcc ggcctttaac gcattgctaa
     1441 caatggtcgc cctcctaaaa atataattcc agcgctcagc cgcatataaa gataccaggt
     1501 tattccagtc tttatccgtt tgaatataat tccataagct aggtggcgat gtgaggttaa
     1561 actggtcatt taaagtgtcc tggatttctt cacatgtcca actacttgta aaatccagat
     1621 gattggtcat ccatattgtt tcccaaaggg ttaagtttgc agtctctata atggtggcat
     1681 cagtacaagt ttcaaaatat tcaacttcca ggcgcaagtc tgtatgaccg gtgccacagt
     1741 catcaacaac ataaggatca acctcgtcga tatgtataag gttagtatca atattaaata
     1801 tactatctct ggaataactt agtaaatatt catcaaattg gaaaggattt aaagtatcgg
     1861 aacctacaat tgcaaaacaa tcataactat cttcggcgca aaattggtaa tagaaatatt
     1921 caggatggta aataattaag gagttagccc aagaaggttg aaaaagcgat acaaaatcct
     1981 ggacatattt aatttcttca gggtaaataa acctaattcc atctgcagta taatgtccaa
     2041 taccatcaat tgattcagga tagtacccta acccggtatc aactggatat ataattgctc
     2101 ttgttgttcc gttttcatcg taatagccac ttatccagtc tcctgcatta ttttttaact
     2161 tcggatttct ccaatttggt tcatactcaa tagcatcaac tgttctaacc tgcaaaatat
     2221 ttgattcatt tagcacagaa agcgcgaagt aatctacatc aatttcgcca gttgccaagt
     2281 tatatagttc ggcatattgt cctcccggcg acatatcttg caacatcatc atgtatcctg
     2341 caaaacaagg atcattgcca acgccaattt cacataaaat ttcgcattgt tgaatattac
     2401 tataaaacat ttctttccac ataagtgtat cggcgctaga tccggcgctg tccatgtttg
     2461 cactgatata ttcttcaaca ctaaggtctc ttatactatc acaataatca caatctaagt
     2521 aacacccggt tgtgtctaaa tcagccattg cgctatcgat aaaatcctct aatgttaaca
     2581 agcagcttgc agtatcaaca tacatttgcg cataaaaatc caaagcttcc tggttaagtg
     2641 ttaatgtttt cgataacata taattaccct gatccaatac cacattaaaa atagcatcat
     2701 cagttaattg tgtatttacc gaatcggtag acaaacaaga aagtgcacta tccaaatttg
     2761 aatttgatgg catgtcacca atttgcagtg taaaaggatt accttcgtta atgagcatct
     2821 ggtcgccgca ttcattggtc agcccaattg tcaaatcata c
//
LOCUS       DAORKW010000485         2859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2011_length_2859_cov_4.916191, whole genome shotgun sequence.
ACCESSION   DAORKW010000485 DAORKW010000000
VERSION     DAORKW010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2011_length_2859_cov_4.916191"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2298
                     /locus_tag="PLB46_14495"
     CDS             <1..2298
                     /locus_tag="PLB46_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77783.1"
                     /translation="TFTISNAEKLPITVLENYDAYVGLTTGGLIVEKVNTTSGKIKSV
                     VKIDETNFNDPTFSIEGLDDNGFYLENTGTGFEEVGADKIILFASGIAEDPDNTYYEI
                     GIPAFSSSLSYKLHGIEAYCTDNKSNRIYADSLVLDTWIDPTIYHTDGFATALNIGKI
                     KVMPDYTSSKSGFTKITINMDKWNITSSNWEINNEGFTMKSGVLDASGVSFAFTDIKI
                     LDGDLIFETSAALGTTTVSLIDILEMDIENTPDFIYNGFNWQVYVGGTPDNPAASIEN
                     LPGLPSNNNDLTLQLLLFESDAAAEFKMNNNSFELFQVTTFTTEEGAMYVHSDEGNKY
                     ITLPGIIDLNLPSISVQPTAYVITNDNGNIATSLEPFGFGMEVNGIVADFNAGDIQFL
                     PNGLIVEGEVSEDPYFSHQVKLYHVTDSTAINTIANQKFQYTSSGQNLQNLFGRMYPL
                     TNSWTNYQFTGDVTGASQVKGRWTFTVYGEITAENQKVDVSNLKMEGSEAMDFDNMGM
                     SFDFANKRLVGSITIDESMPGGGSIAGQANILFDDDGWYFVCGGQVTMPSNPYLNNVS
                     LALLFGDYPIQNETPIKEIFTEYSYDGALPPAFADVIAGFYIDGKVEMPIPYVPNFEI
                     DFVVVSAEFDAGITAGFQLGMNFTEELNTYYIGLMASIHIHAGVGASVVFGCAGVDLD
                     YFVGIELKGQYQSNGEWFTSAEATAAITASAYAGAGLACDSNCEGACAMEEWSGTASV
                     QLKGEVGYQDGSNYHDIGIESVTLE"
     gene            2309..>2859
                     /locus_tag="PLB46_14500"
     CDS             2309..>2859
                     /locus_tag="PLB46_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77784.1"
                     /translation="MKKIIFVCTLCLVCLTQTGFTQNTVAAFASQHGIYLKLENDYPT
                     YKPGVYPEYIIERKKQGDKKFKQIAVVKVPNTYEMFVTNLQSAIEITPEPLLMRQIAA
                     EEIWKKYTTGGYEAISAFSNALFVQLALGIKYYDASPEPNVMYEYVITKKTAAAPTTI
                     YTNVISYPGKMVDYHIKYIDKKE"
BASE COUNT          954 a          457 c          595 g          853 t
ORIGIN      
        1 acatttacaa taagtaatgc ggaaaaatta cctattactg ttttagaaaa ttacgatgcc
       61 tatgttggat taactaccgg aggtttaatt gtagaaaaag taaataccac atcgggcaaa
      121 ataaaaagtg tagttaaaat agatgaaacc aattttaatg acccaacttt tagtattgaa
      181 ggtttagatg acaatggatt ttatttagaa aacaccggta ccggttttga agaagtcggg
      241 gctgataaaa ttattttatt tgccagtggt attgctgaag acccggataa tacatattac
      301 gaaattggca tcccggcatt tagcagttct ttatcgtata aattacatgg tattgaagct
      361 tattgcaccg acaacaaatc gaacagaatt tatgcggatt cactggtttt ggatacctgg
      421 atagatccaa ctatttacca cactgatggt tttgctaccg cattaaatat cggtaaaata
      481 aaagtaatgc ctgactatac ttcttcgaaa tcgggcttta caaaaattac catcaatatg
      541 gataaatgga atatcaccag ttcgaactgg gaaattaaca acgaagggtt tactatgaaa
      601 agtggtgtat tagatgcatc gggtgtaagc ttcgcgttta cggatatcaa aatattagat
      661 ggtgatttaa tttttgaaac atcagcagca ttgggcacca ctactgttag cctgattgat
      721 attttagaaa tggatattga aaacacacct gattttattt acaacggatt taactggcag
      781 gtgtatgttg gaggcactcc tgacaatccc gcagcaagta ttgaaaattt accgggatta
      841 ccaagtaaca acaatgattt aacactacaa ttattattgt ttgaaagtga tgctgccgcc
      901 gaatttaaaa tgaataataa ttcctttgaa ttatttcagg taaccacatt cactaccgaa
      961 gaaggtgcta tgtatgtgca tagcgatgaa ggcaataaat acatcacctt accgggaatt
     1021 atcgatttaa atttaccatc catttcggtg caacctactg cttatgttat taccaacgat
     1081 aatggcaata ttgctacatc gctcgaacca tttggttttg ggatggaagt aaatggtata
     1141 gttgcagatt ttaatgcggg cgatattcaa tttttaccta acggattaat tgttgaaggt
     1201 gaagtaagtg aagatccata tttttcgcac caggtgaaat tatatcacgt aacagacagc
     1261 acagcaatta ataccatcgc taatcaaaaa ttccaataca cgagttcggg acaaaatctg
     1321 caaaatttat ttggccgaat gtatccgctc actaattcgt ggaccaatta tcagtttaca
     1381 ggtgatgtaa ctggcgcgtc acaagtaaaa ggcagatgga catttactgt ttacggagaa
     1441 attactgccg aaaatcaaaa agtagatgtc agcaatttga aaatggaagg aagtgaggcc
     1501 atggactttg ataatatggg catgagtttc gattttgcga ataaacgatt agtaggtagt
     1561 attactattg atgaaagtat gccgggtggt ggcagcatag ccggacaagc caatatttta
     1621 tttgatgacg atggttggta ttttgtgtgt ggtggtcagg ttactatgcc ttcaaatcca
     1681 tatttaaata atgtgagtct ggccttatta tttggcgact atccaattca gaatgaaacg
     1741 cctatcaaag aaatatttac ggaatattct tatgacggtg ctttacctcc tgcttttgca
     1801 gatgttattg ccggatttta tatagatggc aaagttgaaa tgccaattcc ttatgtgccg
     1861 aatttcgaaa ttgattttgt ggtagtgagt gctgaatttg atgcgggtat tactgcagga
     1921 tttcaattag gcatgaattt cacagaagag ctaaatacgt attacatcgg gttaatggcg
     1981 agtattcaca ttcatgcagg tgttggtgca tcggtagtat ttggatgcgc aggtgtggat
     2041 ttagattact ttgttgggat tgaattaaaa ggtcagtacc aaagcaatgg cgaatggttt
     2101 acatccgctg aagcaaccgc agcaattaca gcaagtgctt acgccggagc gggacttgcc
     2161 tgcgacagca attgtgaggg cgcatgtgct atggaagaat ggtccggaac ggcaagtgtt
     2221 cagttaaaag gagaagtagg ttatcaggac ggaagtaatt accacgatat tggaattgaa
     2281 agtgtaacat tagaataaat cagcaaacat gaaaaaaata atatttgtat gcacattgtg
     2341 tttggtttgt ttaacacaaa ctggattcac acaaaataca gttgctgctt ttgcatcaca
     2401 acatggtatt tatttaaaac tggaaaacga ttacccgaca tataaaccgg gtgtttatcc
     2461 ggaatatatt atcgaaagaa aaaaacaagg tgataaaaaa ttcaaacaaa ttgccgttgt
     2521 aaaagtgcca aatacctatg agatgtttgt aaccaattta caatccgcaa ttgaaataac
     2581 acctgaacca ctattgatgc gtcaaattgc ggccgaagaa atttggaaaa aatatacaac
     2641 aggtggttat gaggctatta gtgcatttag taatgcctta tttgttcaac tggcattagg
     2701 tataaaatat tatgatgcca gccctgagcc taatgtgatg tatgaatatg taatcaccaa
     2761 aaaaacagca gcagccccaa caactattta taccaatgtt attagttatc cgggtaaaat
     2821 ggtggattat cacatcaagt acatcgataa aaaagaaag
//
LOCUS       DAORKW010000486         2856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2016_length_2856_cov_5.180650, whole genome shotgun sequence.
ACCESSION   DAORKW010000486 DAORKW010000000
VERSION     DAORKW010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2016_length_2856_cov_5.180650"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1667
                     /locus_tag="PLB46_14505"
     CDS             198..1667
                     /locus_tag="PLB46_14505"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRF77785.1"
                     /translation="MKKNLLATLTFLLTICVVRGQSLETNTTARACMFLHPVEGAYFE
                     VYLVAGANAIKYVPTKPRGYTAGVDVQITITQGDKVLNFDKYRLNTPLVYDTTKIDFS
                     IVDQKRLFVPNVSAVVEVVITDINDSLNSFNYTEVFSSFLKENVQISDVQFADTYKMT
                     SQENAFTKNGVELQPYPINFFPSTRNNIIFYGEVYNTDKYMQDDQFMITYAIRNASTD
                     EFNQEFYQYTKADKQPVFSFVKEMDITDLPGGNYNLLIEVRNKKNELIAQKKVFIQRA
                     NTGAINSWENIKMINTSGTFTDAYSEEQLNYFLDIIKPVASESDRNLIESLSARVEPD
                     MKKKFLYNFWVERDSNDPYKKWLQYLERVKEVNKSFGTPSRAGYKTDRGRVYLQYGPP
                     YDIVPSVNEPGAYPYEIWYYTTLPDKQTNIGFAFYEPSMVSNDYILMHSNARGELHDE
                     RWKVKLYENVASPSEMLDFDNTEVEDKIGGYRAIDMYEF"
     gene            1685..2755
                     /locus_tag="PLB46_14510"
     CDS             1685..2755
                     /locus_tag="PLB46_14510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF77786.1"
                     /translation="MHQEDILQKPLVAVVILSWNGQQYLEQFLPSVVKLSYQPLDIYV
                     ADNASTDGTLDFLKSNYPSIKIIRIQKNEGFAKGYNVALKEVIADYYLLLNQDVEVQP
                     GMIEPLVEKMEADVNIAAAQPKLLAYHNKQQFEYAGAAGGYIDKLGYPFCKGRIFDVA
                     ELDKGQFNTAGEIFWASGAAMFVRAELYKRFKGFDPDFFAHMEEIDLCWRFKRAGYKV
                     FYVPEAVAYHVGGGSLPKENPYKTYLNFRNNLFMIFKNYETQELIWKLPVRLLMENIA
                     FFKALLQGKWKEAFAIFRADWHFVISLPLQFKKRYDNWKNYHRNRIDTTRVRKSGYFS
                     GSIVWQFFIRKRSRFNDLPNEG"
BASE COUNT          916 a          492 c          586 g          862 t
ORIGIN      
        1 gataactggc ctttataaaa tgtgcagatg tatcaaaaca aataacagga tagcctttta
       61 tcagtttcat ccaggctgtc gggggcaggt aatgagcctc ggtgagcaat attttggatg
      121 tgttttctga cattaaaatg atagatattt tacaactcaa ttaaaataaa atgtaatttt
      181 aacataattt tcgttctatg aaaaaaaatc tacttgcgac tttaacattt ttactcacta
      241 tttgcgtagt tcgggggcaa tctctggaaa caaatactac ggcaagagca tgtatgtttt
      301 tacatcctgt tgaaggtgct tactttgaag tgtatctggt agcaggcgcc aatgccatca
      361 aatatgtacc gacaaaacca cgaggttata ctgccggtgt cgatgtgcaa attaccatca
      421 ctcaaggcga taaagtattg aatttcgata aataccgatt gaatacaccc ttagtttacg
      481 ataccacaaa aatcgacttt agtattgtag accaaaaacg tttgtttgtg cccaatgttt
      541 cagcagttgt agaagtagtt attaccgata ttaatgattc actcaattca tttaattata
      601 cagaagtatt cagttcgttt ttaaaggaaa atgtacaaat ttcagatgtg caatttgctg
      661 atacctataa aatgacatct caggaaaatg cattcaccaa aaatggtgtg gaattgcagc
      721 cttatccaat taattttttc ccatcaacac ggaataatat tattttttat ggcgaagtgt
      781 ataataccga taagtatatg caggacgacc aatttatgat tacctatgct attcgaaacg
      841 cttctaccga cgagtttaat caggaatttt atcaatacac caaggctgat aagcaaccgg
      901 ttttttcgtt tgtgaaagaa atggatatta ccgatttacc cggtggtaat tataatctgt
      961 taatagaagt gcgcaacaaa aaaaatgaat taatagcgca aaaaaaagtg tttattcagc
     1021 gtgccaatac aggagcaatt aacagctggg aaaatataaa aatgataaac acctcaggca
     1081 catttaccga tgcctatagt gaggaacaac tcaattattt tttagatatt attaaaccgg
     1141 tggcatcaga atcggaccga aatttaattg agtcgttaag tgctagggta gaacccgata
     1201 tgaaaaagaa atttttatac aatttttggg tggaacgcga ttctaacgac ccgtataaaa
     1261 agtggttgca atatctggag cgtgtgaaag aagtaaataa aagtttcgga acgccatcgc
     1321 gtgccggata caaaaccgat cgtggtagag tgtatctgca atacggtccg ccttatgata
     1381 tcgtgcccag tgttaacgaa ccgggagctt atccttacga aatatggtat tacaccacct
     1441 tgccggataa acaaaccaat atcgggtttg ctttttatga accttcaatg gtttccaacg
     1501 actatatcct gatgcattcc aatgcgcgcg gggaattaca cgacgaacgc tggaaggtga
     1561 aactttacga aaacgtggca tcgccatcag aaatgctgga tttcgacaac acagaagtag
     1621 aagataaaat cggcggctac agggcaatcg acatgtatga attttagtat atctttatgc
     1681 gttcatgcat caggaagata ttctacaaaa gccgctggtt gcagtggtta ttctttcgtg
     1741 gaacggtcag caatacttag agcaatttct gccatctgta gtaaagttgt cctaccagcc
     1801 gctggatatt tatgtggcgg ataatgcttc gacagatggg actttagatt ttttaaagtc
     1861 aaattatcct tccattaaaa taattcgcat tcaaaaaaat gaaggtttcg ctaagggata
     1921 taatgttgcc ttaaaagaag taattgccga ttattattta ttgctcaatc aggatgtaga
     1981 agtacaaccc ggtatgattg aaccattggt ggaaaaaatg gaagcggatg tcaatattgc
     2041 tgctgctcaa ccgaaattac ttgcctatca caacaaacaa caatttgaat atgccggtgc
     2101 tgcaggtggg tatatcgaca aactgggata tccgttttgt aaggggagaa tttttgatgt
     2161 ggctgaatta gataaagggc aatttaatac cgcaggtgaa attttttggg caagcggtgc
     2221 tgcgatgttt gtccgtgcgg agttgtataa aagatttaaa ggatttgacc ctgacttttt
     2281 tgcgcacatg gaagaaattg atttatgctg gagatttaaa cgcgcaggat ataaagtatt
     2341 ttatgttccg gaggcagtag cttaccatgt tggtggtggt tctttgccta aagaaaatcc
     2401 ttataaaaca tatttgaatt tccgcaataa tttattcatg atttttaaaa attatgaaac
     2461 acaggaatta atctggaaat tgcctgtgcg tttattgatg gaaaatattg catttttcaa
     2521 agccttgttg caaggtaaat ggaaagaagc atttgcaatt ttcagagcag attggcattt
     2581 tgtaatttca ttaccactac aattcaaaaa gcgatacgat aattggaaaa attatcatcg
     2641 caaccgaatt gacactacac gtgttcgcaa atcaggttat ttttcaggca gcatcgtatg
     2701 gcagtttttc atccgcaagc gcagccgctt caacgatctt ccaaatgagg gatgagggat
     2761 ttaaatgtgc tgatgtgttt atttgctaat gtgctaatta accctagttg tcgcagacaa
     2821 cgttgtcgtg gagtctttga ctccagacgc cacccc
//
LOCUS       DAORKW010000487         2844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2030_length_2844_cov_5.120473, whole genome shotgun sequence.
ACCESSION   DAORKW010000487 DAORKW010000000
VERSION     DAORKW010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2030_length_2844_cov_5.120473"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /gene="lnt"
                     /locus_tag="PLB46_14515"
     CDS             <1..1488
                     /gene="lnt"
                     /locus_tag="PLB46_14515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRF77787.1"
                     /translation="TSFKVWLSLYFGLLLWNLLTTWWVSNASVAGGLFANMANPILMT
                     VPLMLARNTGKRLGENAGLLAFIVYWMSFEFIHLRWELTWPWLSLGNVFALNHTLIQW
                     YEYTGVFGGTLWVLVANVLIYKIVSKFIFTQTENNQQKKKTSLMYGTIFLLWIIIPIV
                     ISKNIYNHYVEKGTSTNVTVVQPNFDPYTEKFTIPYSIQMDKMLSLSLDGITDSTSFL
                     VWPETAIQTEIWLDKLPYEKPVRDIQKAIDSFPNLTAVVGINGFERYYDKSKVSPTAR
                     TFVTHNSKRDTIIYDIYNTALAINGNGPQGYYHKSKLVPGVERMPYPGFFAFIGSWAI
                     ELGGISGSLGIQKERTVFFNKDSIGVAPVICYESIFGEYVSDYVNKGAGLIFIITNDG
                     WWGKTNGYLQHNEYAKLRSIETRRSIARSANTGTSCFINQRGDISQATEWREDAVINQ
                     SLKVNTTITFYTKHGDYLARIALIISGLLAITALVNRVRSKIKSN"
     gene            1497..2300
                     /locus_tag="PLB46_14520"
     CDS             1497..2300
                     /locus_tag="PLB46_14520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRF77788.1"
                     /translation="MMMDYVQLEQLCSSVRDVVKQVGLDIAISRKRIDEIVIEKKGVR
                     DFVTEVDRQAEQTLVKHLQNLLPEAGFVTEEKTIAQSDKPYNWIIDPLDGTMNFVHGI
                     PAYSVSIGLEFQHEIILGVVYEIVHDEMFYSWKNAPSFCNNKIIHISDCKQLQDALIA
                     TGFPYIRNDERTTKISATLKYFLDNGRDIRRIGTAATDLCYVACGRMDVYYEGFLNIW
                     DVAGGIIIVQNAGGFVSDFSGGNNFSKGEIVACSPVIKDVVLTGVALMP"
     gene            complement(2352..>2844)
                     /locus_tag="PLB46_14525"
     CDS             complement(2352..>2844)
                     /locus_tag="PLB46_14525"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRF77789.1"
                     /translation="VDGFFQVMQLVSSAAFSLGHGSNDAQKTAGIIILLLASAGHASI
                     DAPPQWVFYACYTVIGLGTLIGGWKVVKTMGHNLTPLKPMGGFCAETAGAITLFGTAA
                     MGIPASTTHTITGSIMGVGAAKRVSAVRWSVVTKIMWAWILTIPAAMIMSGLIYYIVS
                     FFV"
BASE COUNT          945 a          467 c          546 g          886 t
ORIGIN      
        1 acttctttca aggtttggtt aagcctgtat tttggcttgt tgctttggaa tctgctcacc
       61 acttggtggg taagtaatgc aagtgtagca ggaggtttgt ttgccaatat ggccaacccg
      121 attttaatga ccgtgccgct tatgcttgct cgaaataccg ggaaacgtct tggtgaaaat
      181 gcaggcttgt tggcatttat tgtttactgg atgagttttg agtttatcca tctccgctgg
      241 gaattaacct ggccatggtt gtcgctggga aatgtatttg cacttaatca tacattgatt
      301 caatggtacg aatacacggg tgtttttggt ggtactttat gggtattagt agccaatgtg
      361 ctgatatata aaattgtgtc taagtttatt tttacccaaa ctgaaaacaa tcaacaaaag
      421 aaaaaaacat ccttaatgta cggaacaatt ttcctccttt ggattattat tccgattgta
      481 atttctaaaa acatttataa ccattatgta gaaaaaggaa catcaactaa tgttactgtt
      541 gtacaaccaa atttcgaccc atatacagaa aaatttacta ttccctacag tattcaaatg
      601 gataaaatgt tatcgctttc gctagatggc attaccgatt caacatcatt tttggtgtgg
      661 ccggaaacgg caattcaaac tgaaatttgg ttagataaac tgccttatga aaaaccggta
      721 agagatattc aaaaagcaat tgacagtttc ccgaatttaa ctgctgttgt cgggataaat
      781 ggatttgaac gatattacga taaaagtaaa gtttcaccaa ccgctagaac atttgtaacg
      841 cataattcaa aacgtgatac aattatttac gacatttata atacagcact tgccataaat
      901 ggtaacggac ctcagggtta ttatcataaa tctaaattag tgcccggtgt tgaacgaatg
      961 ccatatcccg gtttttttgc atttataggg tcatgggcaa ttgaactcgg cggaatttcc
     1021 ggaagtttag gtattcaaaa agaacgcaca gtgtttttta ataaagacag tattggggtt
     1081 gctcctgtaa tttgttatga aagtattttt ggagaatatg tttctgacta cgtgaataaa
     1141 ggtgcgggtt taatttttat tattactaac gatggttggt ggggaaaaac aaatggatat
     1201 ttacaacata acgaatatgc caaattgcgc agcatcgaaa caagaagaag tattgcccgc
     1261 tctgcaaata ccggtacatc atgttttatt aaccaacgtg gagatatttc acaggcaacg
     1321 gaatggcgag aagatgctgt aataaatcaa tctttaaaag ttaatactac aatcactttt
     1381 tacactaaac atggcgacta tctagcccgt attgctttaa ttatttccgg attattagct
     1441 attacagcat tagtgaacag ggttagatca aaaattaaat caaattaaat taaattatga
     1501 tgatggatta tgtgcagctt gaacaattgt gttcgtcagt aagggatgtt gtaaaacagg
     1561 ttgggcttga catcgccata tccagaaaac gaattgatga aattgtaatt gaaaaaaaag
     1621 gggtacgcga ttttgtaact gaagtggaca gacaggcaga acaaacttta gtaaaacact
     1681 tacaaaattt attacctgag gcaggatttg taacggaaga aaaaacaatc gcccaatctg
     1741 ataaacctta taattggata atagatccat tagatggaac tatgaatttt gtgcacggaa
     1801 ttcctgcata tagtgtaagt ataggactgg aatttcaaca cgaaataata ttaggtgtag
     1861 tatatgaaat tgtgcacgat gaaatgtttt attcctggaa aaatgcacct tctttttgta
     1921 ataataaaat aattcacatt tctgattgta aacaattaca agatgcatta attgcaacag
     1981 gttttccata tattagaaat gatgagcgaa caacaaaaat ttcggcaaca ctaaaatatt
     2041 ttttagacaa tggtcgtgat atacgcagaa taggtacagc agcaaccgat ttatgttatg
     2101 ttgcgtgtgg cagaatggat gtttattacg aaggattttt aaatatttgg gatgttgcag
     2161 gtggtattat cattgtacaa aatgcaggtg gatttgtttc ggatttttca ggagggaata
     2221 attttagtaa aggtgaaatt gtagcctgca gtcctgtaat aaaagatgtt gttttaacag
     2281 gtgttgcctt aatgccataa aaaaacgcaa ccgttaaacg attgcgtttt acaaattata
     2341 ttgattttcg tttatacaaa aaatgaaacg atataataaa ttaatccgct cataatcatt
     2401 gctgcaggaa tagttaaaat ccaagcccac ataattttag ttactaccga ccaacgaaca
     2461 gcactaacac gtttagctgc cccaactccc ataattgaac cggtaatagt atgtgtagta
     2521 gatgctggaa ttcccattgc tgcagtacca aataaagtaa tggcaccggc agtttctgca
     2581 caaaaaccac ccataggttt taatggcgtt aaattgtgtc ccattgtttt tacaactttc
     2641 catcctccta ttaaagtacc caatccgatt actgtataac aggcataaaa tacccattgc
     2701 ggtggggcat cgatactagc atgccctgca gatgcgagaa gtaaaataat aattcccgct
     2761 gttttttgag catcgttact accatgacct aaactaaatg cagctgaaga aactaattgc
     2821 attacctgaa aaaatccatc tact
//
LOCUS       DAORKW010000488         2837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2036_length_2837_cov_5.185478, whole genome shotgun sequence.
ACCESSION   DAORKW010000488 DAORKW010000000
VERSION     DAORKW010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2036_length_2837_cov_5.185478"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /locus_tag="PLB46_14530"
     CDS             <1..1759
                     /locus_tag="PLB46_14530"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRF77790.1"
                     /translation="IIRILFYNFASQFSIMMHVAYEVAMPNPATHVFKFTLHIKGVTE
                     EFVHLELPVWTPGSYLVREYAKNVNALKATDTQGDTLPVVKTQKNSWAVYAAGNDFTV
                     TYEIYAFEESVRTCWLDSSHASIIPAACFLWVAGYEGGVEITIHPHESFKKIATALPN
                     TQNNVWQLIAADKDTLYDSPIEIGNHETYTFEAAGIPHTLAIYGEGNHNAQQIITDLI
                     KIIDTEVNIFQSHPCDKYLFILLNSHTQRGGLEHLFSTSLIFKRFGYSARTSYLEFIS
                     LCAHEYFHLWNVKRLRPAALGPFNYGEENYTTGLWVAEGFTSYFDDLIVYRAGIYTEN
                     EYLNVVEKNINDAENAPGKHVQSVAEASFDAWIKYYRQNENSNNSQVNYYVRGGVLAL
                     LLDLMIIRYSKGEHNINDVMRDAYNQFYLQRNQGFTEAELKTILEHYTGENLDWFYDN
                     HVFGTTPIDYSIFAAFGLLLVNQNEGKLELDLGIVINDKNTIQSIRKNSAGEKGGLNV
                     NDEIIAINNFRYTSGLLNQFTENKSAGDTIQLTISRSGVLQTFEVTLQNTDKVDYKLT
                     PLESAGAAQKSLYEFWLKR"
BASE COUNT          886 a          527 c          554 g          870 t
ORIGIN      
        1 aataattcgc attttgtttt acaattttgc atcacaattt tcaattatga tgcacgttgc
       61 atacgaagta gccatgccga atccggcgac acatgttttc aaattcaccc tacatattaa
      121 gggcgttacc gaagaatttg ttcatttgga attgccggtc tggacacccg gttcttattt
      181 ggtacgtgag tatgcaaaaa atgtaaatgc actaaaagct actgacacac aaggtgatac
      241 attacctgtt gttaaaacac aaaaaaatag ttgggctgtt tatgctgcag gaaatgattt
      301 taccgttacc tacgaaattt atgcatttga agaaagcgtg cgcacctgtt ggttagatag
      361 cagtcacgct tcaattattc cggctgcgtg ttttttatgg gttgccggat atgaaggtgg
      421 ggttgaaatc actattcatc cgcacgaaag tttcaaaaaa attgccactg cattaccaaa
      481 cacacaaaat aatgtttggc aattaattgc tgctgataaa gataccttat acgatagccc
      541 aatcgaaatt ggcaatcacg aaacctacac atttgaagcg gcaggcattc cgcatacttt
      601 agccatttat ggagaaggca atcacaatgc tcaacaaatt attaccgatt taattaaaat
      661 tattgatact gaagtaaaca tttttcaaag tcacccatgc gataaatatt tatttatttt
      721 attaaattct catacacaac gtggtgggtt ggaacatttg ttttcgacaa gtttaatatt
      781 taaacgtttt ggatattcag cacgtacctc ctatttagaa tttatcagtt tatgtgcgca
      841 tgaatatttc cacctttgga atgtaaaacg tctgcgccct gcggctttgg gaccatttaa
      901 ttacggagaa gagaactata caacaggtct ttgggtggct gaaggtttca ccagttattt
      961 tgatgacctc attgtttatc gtgcaggaat ttataccgaa aacgaatatt taaatgtagt
     1021 tgaaaaaaat attaatgatg cggaaaatgc accgggtaaa catgtacaaa gtgttgcaga
     1081 agcaagtttt gatgcatgga taaaatatta tcgtcaaaac gaaaacagca ataattccca
     1141 ggtaaattat tatgttcgcg gaggtgtttt agcattgctc ctcgatttaa tgattattcg
     1201 ttacagcaaa ggcgaacata atatcaatga tgtaatgcgc gacgcttata accaatttta
     1261 tttacaacgc aaccagggct ttaccgaggc agaactcaaa actattcttg aacattacac
     1321 cggcgaaaat ttagattggt tttacgataa tcatgtattt ggcacaactc caattgatta
     1381 ttccattttt gcagcattcg gattgttatt agtcaatcaa aatgaaggta aattagaatt
     1441 agatttaggt attgttatta acgataaaaa taccatccaa tctatccgca aaaacagcgc
     1501 aggtgaaaaa ggcggattaa atgtaaatga tgaaataatt gctattaata atttccgata
     1561 tacaagcggt ttacttaatc agtttactga aaataaatct gccggagata ccattcaatt
     1621 aacaattagc cgcagtggtg tactgcaaac atttgaggta acactacaaa ataccgacaa
     1681 agtggattat aaattaactc cattggaatc agcaggtgct gcgcaaaaat cgctgtatga
     1741 attttggttg aagcgatgag gggttattaa tttttggagt aaaaattgta ccggtctgga
     1801 gaccggggtg gcgtctggag tcaaagactc cacgacaacg ttgtctgcga caacatgggt
     1861 gcgcgaagta attattcgta aattcgaatt taattttaat ttctcggtga actttgtgtg
     1921 tattttctct gagtcccgat agctatcggg attgtggttt cggctgttca aaaaataaat
     1981 gcaagtaatt ttctattaaa cccaatcact cattattcat cactcattgc tcatattttt
     2041 ttccggtccg gagaccgggg tggcgtctgg agtcagagac tccacgacaa ctcggagcct
     2101 ggaaatttaa taaccatttg ataatttagc cttgtcagct aagtacggat ttttttgtaa
     2161 ataaactata ttatttccgc taataaatcc gcgttatgtt tacctaccac ctaggtaggg
     2221 gtggcattat cttatccaag taatctcatc taacgaaata atccatcccc tttaggttta
     2281 cctgctggat aaagcaaggg tgacattatt ttaactaccg aacttcgagg aacccaaaac
     2341 cacgttaggt ttacctgact gactaggcag gggtgacatt attttaacta cataaccagc
     2401 aggaacccaa accccgttag gggtgacatt atttttactc cgaatgccaa aaatttcggt
     2461 aaacgactta gcaaacacct aatccaaaaa cacacgatcg gtctggagac cggggtggcg
     2521 tctggagtca gagactccac gacaacgttg tctgcgacaa catgggtgcg cgaagtaatt
     2581 attcgtaaat tcgaatttaa ttttaatttc tcggtgaact ttgtgtgtat tttctctgag
     2641 tcccgatagc tatcgggatt gtggtttcgg ctgttcaaaa aataaatgca agtaattttc
     2701 tattaaaccc aatcactcat tattcatcac tcattgctca tatttttttc cggtccggag
     2761 accggggtgg cgtctggagt cagagactcc acgacaactc ggagcctgga aatttaataa
     2821 ccatttgata atttagc
//
LOCUS       DAORKW010000489         2835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2040_length_2835_cov_5.560432, whole genome shotgun sequence.
ACCESSION   DAORKW010000489 DAORKW010000000
VERSION     DAORKW010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2040_length_2835_cov_5.560432"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLB46_14535"
     CDS             complement(<1..553)
                     /locus_tag="PLB46_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77791.1"
                     /translation="MKHDKLTAYSLMAGSFLFAKNDLMAEIVYTDIVPDIVLENDDDI
                     DLDIDLNGINDFRFFLDSGRFAVWGGYYGSYSFSSILSSFKNIRALCFDNNAMVTQYF
                     TSTSKGTIYFFASLDPLASNVMINEDAFNLVDASQIHIARKQRTVSATSYEPLGDWLY
                     SNWSNPWNYNLLHGEDRFAGIQFY"
     gene            complement(711..2396)
                     /locus_tag="PLB46_14540"
     CDS             complement(711..2396)
                     /locus_tag="PLB46_14540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77792.1"
                     /translation="MKTILATTIALAVLQSPQLFAQTTIRANEQQFENETMQSPYLKN
                     RWDNKKTSAPFFEQRIVEGDTVIISIRTMQVNVNDAASNIVDDAGNEPSIAIDINDTL
                     HIAIGWRQFDDIASNFRQAGWSYSSDGGLTWNMSIIDEGVFRSDPVLDYDLQGNFYYN
                     SLTPYPDWECDVYKSTDGGATWDEGTYAYGGDKQWMAIDRTNGAGSGNIYAIWSADYS
                     ACIPGNFTRSTDGNLSYESCDHVEAEPYYGMPAVDADGYLYVVGGGTSSTLTVTRSLN
                     AQIPGATIEWDDPVNVDLDGSLSGWQDINPEGLIGQANIDIDRSGGPGHGNIYVLAPV
                     DPYTGPDDADISFAKSTDGGLTWSDPIWINDEKNDGNTHWFGTMSVAPNGRIDVIWLD
                     TRDGGGVDYSALYYSYSVDQGDTWSANMKLSPSFDPHVGYPSQNKMGDYFDMTSDDKG
                     AHLAWANTLNGEEDVYYSRILLTEAAIVNIDASTVASIAITPNPGSGIFHISGVVDAN
                     EITVVNLVGDIIMTQAVNGSQASIDITNFADGIYFVKIAFGDGRYYTKRFIKQ"
     gene            2552..>2835
                     /locus_tag="PLB46_14545"
     CDS             2552..>2835
                     /locus_tag="PLB46_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77793.1"
                     /translation="MRGTVFCWVLISELGYCTTKKPQNPTKFGTEMRYKLKFITSNVT
                     GLYRNIILIALIFFEKVQLFIGICLGEPIWSGSNQVIEAFNPAISKNPKFS"
BASE COUNT          900 a          592 c          484 g          859 t
ORIGIN      
        1 cgtaaaactg aatacccgca aaccggtcct ccccatgtaa taaattataa ttccatggat
       61 tactccaatt ggaatataac caatcaccaa gtggttcgta agaagttgca gaaactgttc
      121 tttgttttct ggctatatga atttgacttg catcaacaag attaaatgca tcctcattta
      181 tcattacatt ggaagccagc gggtcaagac tggcgaaaaa ataaatggtt ccttttgaag
      241 tggatgtaaa atattgtgtt accatagcat tattatcaaa acaaagtgca cgaatatttt
      301 taaaagaact caatatggaa gaaaacgaat aggaaccata ataaccaccc cacactgcaa
      361 accgaccact atctaagaaa aatcgaaaat catttatacc atttaaatca atatcaaggt
      421 caatgtcatc atcattttcc aatacaatat caggaacaat atcggtgtaa acaatttctg
      481 ccatcaaatc atttttagca aataaaaacg aaccggccat tagggaatat gctgttaatt
      541 tatcgtgttt catgaggtag aattgatgct aaagttaata catatgttta aatttttcaa
      601 agcatgttag ccgcatcacc ccgttgtcgt ggagtctcca gactccagac gccaccccgg
      661 tctccagacc gattaagcta ttgcaaatag ttgaattaaa gtataatatt ttattgctta
      721 ataaaacgtt tggtataata tcgtccatcg ccaaatgcaa tttttacaaa ataaatacca
      781 tctgcaaaat tggtaatatc tatagatgct tgtgaaccat tcaccgcttg tgtcataata
      841 atatcaccca caagatttac aactgtaatt tcatttgcat caacaacgcc tgaaatatga
      901 aaaatgcctg aacccggatt tggtgttata gcaattgaag caactgtaga agcatcaata
      961 tttactatag ctgcttctgt aagtaatatt ctgctgtaat acacatcttc ttcgccgttt
     1021 aatgtatttg cccatgccag atgtgcacct ttatcatcgg aagtcatatc aaaataatca
     1081 cccattttat tttgtgatgg ataaccgaca tgcggatcaa atgatggaga taatttcata
     1141 tttgccgacc atgtatcacc ctgatcaaca gaataagaat aataaagtgc cgaataatca
     1201 acaccaccac catcgcgggt gtcgagccat atcacatcta ttctgccatt tggtgcaacc
     1261 gacatagtac caaaccaatg tgtattgcca tcattttttt catcgttgat ccaaatcggg
     1321 tctgaccatg ttaatccgcc atcagtactt ttagcaaaac taatatcagc atcatcagga
     1381 ccggtataag gatctaccgg agctaaaaca taaatgttgc catgtccggg tccgcctgag
     1441 cgatcaatat caatattcgc ctgacctatc aatccttccg gattaatatc ttgccatcca
     1501 cttaaacttc catccagatc aacatttaca gggtcgtccc attcaatagt ggctcccgga
     1561 atttgtgcat tcaacgaacg agttacagtt aatgtggaag aagtgccacc accaacaaca
     1621 tataaataac catctgcatc cactgccggc ataccgtaat agggttctgc ttcaacatga
     1681 tcacatgatt cataactcaa attaccatca gtagaccgcg tgaaatttcc cggtatacaa
     1741 gcactgtaat ccgccgacca aattgcataa atatttccgc tacccgctcc gttcgtgcgg
     1801 tcaatggcca tccattgttt atcaccacca taggcatatg tgccttcatc ccatgtggcc
     1861 ccaccatcgg tgcttttata tacatcacat tcccaatcgg ggtatggcgt gagactatta
     1921 taataaaaat tgccctgcaa gtcgtaatct aaaacagggt ctgagcggaa aacgccttca
     1981 tcaataatgg acatattcca ggtaagccct ccgtcggacg aatacgacca accggcttgt
     2041 ctgaaattac ttgcaatatc atcaaattgc cgccatccga tggcaatatg taaagtgtcg
     2101 ttaatatcaa tggcgatgct gggttcattt cccgcatcat ccacaatatt ggaagccgca
     2161 tcatttacat tcacctgcat cgttcgaata gaaatgataa cagtatcacc ttctacgata
     2221 cgttgctcaa aaaaaggcgc agacgttttt ttattatccc acctgttttt caggtaagga
     2281 ctctgcatgg tttcattttc gaattgttgt tcatttgcgc gtatggtggt ttgcgcaaat
     2341 aattgcggtg actgcagtac agccaaagca atagtagtag ctaaaattgt tttcatggtg
     2401 gaaatttaaa actaatttat gtcataacta ttacgacata tttcaaagat gaaaattttt
     2461 aaattttcgt tgcattagtt aataatgagg aggcttgtga tggatgctaa tatcctacat
     2521 cgttttcgtt ttttaaactt agtattggtg aatgcgtgga actgtgtttt gctgggtttt
     2581 gatttctgag ttaggttatt gcaccacaaa aaaaccgcaa aaccctacga aatttggaac
     2641 ggaaatgagg tacaagctta aatttattac ttcgaatgta acagggttat accggaatat
     2701 tattttgatt gctttaatct tttttgaaaa agttcaatta tttataggta tatgtttagg
     2761 cgaaccaata tggtctggtt ctaaccaagt tattgaagcg tttaatcctg caatttctaa
     2821 aaatccaaaa ttttc
//
LOCUS       DAORKW010000490         2834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2041_length_2834_cov_5.574667, whole genome shotgun sequence.
ACCESSION   DAORKW010000490 DAORKW010000000
VERSION     DAORKW010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2041_length_2834_cov_5.574667"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PLB46_14550"
     CDS             complement(<1..1109)
                     /locus_tag="PLB46_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77794.1"
                     /translation="MKNKKNKSSKLAILLMMICLKSFGQAPGIEWQNTIGGTGGDYLQ
                     SISQTTDDGYIIGGYSYSGKNGDKTEPTQGNADYWVLKLNNAGSIQWQNTIGGNDNDL
                     LLAVQQTSDGGYILGGYSWSGISDDKTEPAMEYDYWVVKLNNSGVIEWQNTIGGIALD
                     FLTCLHQTADGGYILGGYAWSGISGDKTEVSQGGFDYWVVKLNSLGSIEWQNTIGGNG
                     YDYLYSIKQTSDGGYIIGGESNSSISGDKTENMVGVPYNYDYWVLKLDNIGEIEWQNT
                     IGGSNNDYLRCVQQTFDGGYILGGYSQSGLSGDKSESSLGSYDYWVVKLNNSGLIEWQ
                     NSLGGSSEDYLYSVQQTADSGYILGGNSMSGISFD"
     gene            complement(1128..>2834)
                     /locus_tag="PLB46_14555"
     CDS             complement(1128..>2834)
                     /locus_tag="PLB46_14555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77795.1"
                     /translation="GGYLIGGRSFSGISGDKTEASIGDFSTYDFWVLKLNSSGNIEWQ
                     NTIGGSSTDDLTSLQQTSDGGYILGGYSTSGVSGDKTEASKGAGDYWVVKLNSSGFIT
                     WQNTIGGSGEDVLISVSQTSDGGYILGGSSCSNISADKSENNITGWCEWKDYWIIKLN
                     GSGNIVWDNTIGGTENDECVSVFQTAEGGYIIGGYSNSGTSTDKSENNFSGGEYGWDF
                     WIVKLDYTGTIIQWENTIGSAGPWPYGDDYLNSSVQETADGGYIIGGTSYGGISGDKT
                     AANIGSFDFWVIKLLPFCTVGEAELCNHLDDDCDGLIDEGEITETIIISPTGSTSFCT
                     GGSVILNATYSGSTVQWKRNGINIPGATSPSITASKTGTYTCETFSDCDTTLSAAIVV
                     TENKNPNATISAGGPTTFCAGGSVILTEVAVAGCTYQWYKGATPIAGATSLTYTATTS
                     GNYKCRVTKAVTGCYKNSNAIAVSVPCREGLPAGEAGDNLIENTFSLYPNPANNFITI
                     ETDFSTEKTIYITDALGQIVKTIITSENYITIDLQGIASGIYFIKMEDGINSVSQKFV
                     KQ"
BASE COUNT          849 a          642 c          413 g          930 t
ORIGIN      
        1 ttatcaaaag aaattccaga catagaattc cctcctaaaa tataaccaga atctgcagtc
       61 tgttgaacag aatacaaata atcttcgcta cttcctccta aagaattttg ccattcgatt
      121 aagccactat tattaagttt cacaacccaa taatcataac tacccagtga actttcagac
      181 ttatctccac tcaatccaga ttgggaatat cctcccaaaa tatatccacc atcaaatgtt
      241 tgttgaacac aacgtaaata atcattatta ctacctccta ttgtgttttg ccattctatt
      301 tcgccgatat tatctaattt caaaacccaa taatcatagt tataaggtac acctaccata
      361 ttctcagttt tatctccaga aatactagaa ttagactcac ctcctataat gtagccaccg
      421 tctgaagttt gttttataga atataaataa tcatagccgt tcccaccaat ggtgttctgc
      481 cattcaatac tacctaaact gttcaatttc acaacccaat aatcaaaacc tccttgtgat
      541 acttcggttt tatcacctga aattccagac catgcatatc cgccaagtat atatccacca
      601 tctgctgttt ggtggaggca tgttaaaaaa tctaatgcta ttccaccaat cgtattttgc
      661 cattcaatta caccgctatt gttgagttta actacccaat aatcatattc catagctggc
      721 tcagttttat catcagatat tccagaccaa gagtaaccac ccaaaatata acccccatca
      781 gaagtttgtt gtacagcaag aagcaaatca ttatcattac ccccaatcgt attttgccac
      841 tgaatactcc ccgcattatt tagtttcaac acccaataat cagcatttcc ttgtgttggt
      901 tcagttttat cgccattctt accagaataa gagtatcctc ctattatata cccgtcatca
      961 gtggtttgac taatagattg taaataatct ccacctgtac ctccaattgt attttgccat
     1021 tcaataccag gtgcctgtcc aaatgatttt aaacaaatca tcattaaaag gattgctaat
     1081 ttggatgatt tatttttttt atttttcatt ttttttattt gggattttta ttgtttaaca
     1141 aatttctgcg acaccgaatt aataccatct tccattttaa taaaataaat accggaagcg
     1201 atgccctgta aatcgatggt gatataattt tcagatgtga taattgtttt tacaatttgg
     1261 ccaagtgcat ctgttatata aattgttttt tcagttgaaa aatctgtttc aattgtgatg
     1321 aaattattgg ccgggtttgg atagagcgaa aaagtatttt cgataagatt atctcctgcc
     1381 tcgccggcag gcaggccttc cctacacggc acactcaccg caatcgcatt agaattttta
     1441 taacaacctg taacagcttt ggtaacacga catttataat tacctgaggt agttgctgta
     1501 tacgttaatg aagttgcacc tgcaattggt gttgcgcctt tataccattg gtaagtacat
     1561 cctgcaaccg caacttcggt taatattacg ctgccacctg cacaaaatgt tgttgggcct
     1621 ccggcagaga ttgtcgcatt gggattttta ttttcagtga caactattgc tgccgataat
     1681 gttgtatcac aatcgctaaa tgtttcacaa gtataggttc cagtttttga agccgtaata
     1741 gaaggtgaag ttgcaccagg tatatttatt ccattccttt tccattgtac tgttgaaccg
     1801 gagtatgttg catttaaaat aacagaaccc ccagtgcaaa atgatgtaga accagttgga
     1861 gaaataatta ttgtttctgt tatttcccct tcatcaatta atccatcaca atcatcgtcg
     1921 aggtggttgc acaattcagc ctcacctacg gtgcagaaag gtaagagttt tataacccaa
     1981 aagtcaaatg atccaatgtt tgcggctgtt ttatcgccgg aaattccacc gtatgatgtc
     2041 cctccaataa tataacctcc gtcagcagtt tcttgtacag aactattaag gtagtcatcc
     2101 ccataaggcc atggaccagc agatccgatg gtattttccc attgaatgat tgttcctgta
     2161 taatcgagtt tcacgatcca aaaatcccat ccgtattctc cgcctgagaa attattttca
     2221 gatttatcag tgcttgttcc gctgtttgaa tatccaccaa tgatataacc accctctgct
     2281 gtctgaaaga cactaacgca ttcgtcattt tcagtgcctc caatagtatt atcccaaaca
     2341 atatttccgg atccgttcaa tttaattatc caataatcct tccattcgca ccaacctgta
     2401 atattgtttt cagatttatc ggcagaaatg tttgagcatg aacttcctcc taaaatatat
     2461 ccaccatcgc tagtttgact aacagaaatt aaaacatctt caccactgcc accgattgta
     2521 ttctgccagg taataaaacc agagctattt aatttcacaa cccaataatc acctgcacct
     2581 ttgcttgctt ctgttttatc acctgatact cctgaagttg aatacccacc taaaatataa
     2641 cctccatctg aggtttgttg caatgaagtt aaatcgtcag tagaacttcc cccaatagta
     2701 ttttgccatt caatgttccc tgaactattt agtttcaata cccaaaaatc ataggtgctg
     2761 aagtcaccaa ttgaggcctc tgttttatcc cctgaaattc cagaaaatga tcttcctcca
     2821 attaggtatc cacc
//
LOCUS       DAORKW010000491         2833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2045_length_2833_cov_4.749460, whole genome shotgun sequence.
ACCESSION   DAORKW010000491 DAORKW010000000
VERSION     DAORKW010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2045_length_2833_cov_4.749460"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..832)
                     /locus_tag="PLB46_14560"
     CDS             complement(47..832)
                     /locus_tag="PLB46_14560"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF77796.1"
                     /translation="MPAIKRISTSCLLLIVIFTISISALRTPSIQKTLKKDGYVAVNE
                     LPNGNQLFFSTTEVSNAQYRAFISYLKTNQRSNDLVIAQVDSAAWTNTFAYNAPYVNY
                     YFQHTAYDNYPVVNITYEGAQLYCDWLTAQYKNKGYTVKVRLPEADEWITAAQGGNSQ
                     NTFGWEGNNLTDKKGNPLANYKWNMEKTIIVSPVNSYQPNGFGIYNMSGNVAEMLQTK
                     GNHKGGSWFSEQQYVTVLGEDEYNNITTASPFIGFRTVIEVSK"
     gene            complement(881..1756)
                     /locus_tag="PLB46_14565"
     CDS             complement(881..1756)
                     /locus_tag="PLB46_14565"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF77797.1"
                     /translation="MPWKSHTNNTEIVVNYLNNQLMRKTLLLTFICGLLIAFGTSFTF
                     KQSEKKFLKKRGFEFVPSGTTTIDGKTVSIQGFYMLESEVSNLAYREFLYDLEKNGKT
                     EALAIAAVAADKWLLPNMQMDVMVENYHTNIAYNAYPVVNISQEGARLYCRWLEEKFA
                     EQGYKLKVRIPEMAEWVYAAKGGNESNMYAWEGNSLRNKKGAYLANFKTDKTGDDGGY
                     ITAITKSYFPNAYGLYNMCGNVSEWVNDTAMSKGGNWWSDAEFLKIDAKQEFGNSTQP
                     SPFIGFRPVITATVY"
     gene            complement(1946..>2833)
                     /locus_tag="PLB46_14570"
     CDS             complement(1946..>2833)
                     /locus_tag="PLB46_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRF77798.1"
                     /translation="GKTTRSADPNRRLIGDDEHGWTEDSVFNFEGGCYAKCVNLTKEN
                     EPQIFNAIKFGAILENIEFYPNTRNVDFANITKTENTRVSYPINYIDNIMTPSKGGLP
                     KNIFFLTCDAFGVLPPISKLSPGQAMYQFLSGYTAKVAGTEAGVTEPQLTFSTCFGAP
                     FLPLHPTKYAEMLGEKMRKHNVNVWLINTGWTAGPYGTGHRMKLSYTRAMITAALNGE
                     LDNVTFTAHDIFGLLMPTTCPNVPADILNPRNTWSDKTAYDQKANNLAAQFVKNFEKY
                     ADKANAEILSAAPKVSASV"
BASE COUNT          850 a          533 c          503 g          947 t
ORIGIN      
        1 gcgaataaaa atgcaacaag tagttttttt atcataagtt atcttattat tttgaaactt
       61 caattacagt tctgaaacca ataaatggag atgctgttgt gatattatta tattcatctt
      121 cacctaatac ggttacatat tgttgttcac taaaccaact tccaccttta tgattgcctt
      181 ttgtttgtaa catttcagca acattgccac tcatattata aattccgaaa ccatttggtt
      241 gataggaatt taccggtgaa actattattg ttttttccat attccattta taattagcca
      301 atggattgcc ttttttatca gttaaattat tcccttccca tccaaatgta ttttgcgaat
      361 ttccaccttg agcagcagta atccattcat ccgcttcggg caaacgtact ttaacggtat
      421 agcctttatt tttatattgt gctgttaacc aatcgcaata taattgagcg ccttcatagg
      481 taatatttac caccggataa ttgtcgtaag cagtatgttg aaaataataa ttcacatacg
      541 gcgcgttata cgcaaatgta ttggtccaag ctgctgaatc tacttgagca ataaccaaat
      601 cgttggagcg ttgattagtt ttcagataag aaataaatgc tcggtattgt gcatttgaaa
      661 cctctgttgt tgaaaaaaat aactgattgc cattgggtaa ttcatttacg gcaacataac
      721 catctttttt taatgttttt tgtatggaag gagttcgcaa agccgatata cttattgtaa
      781 aaattacaat aagtagtaaa catgacgtag aaatccgttt tatagctggc atattttagt
      841 taaaaataaa cataaaaatg caataagcaa ttttgggatg ttaatatacg gtagccgtaa
      901 ttaccggtct gaacccaata aacggagagg gttgcgttga gttgccaaat tcctgtttgg
      961 catctatttt caaaaactcc gcatcactcc accagttgcc acctttagac atggcggtat
     1021 cgttaaccca ttcgctaaca ttgccacaca tattgtagag cccatatgca ttgggaaaat
     1081 aggatttcgt aatcgctgta atgtaaccgc catcatcccc ggttttatcc gttttaaaat
     1141 tagccagata ggcacctttt ttattgcgaa gcgaattacc ctcccatgca tacatgttgg
     1201 attcgtttcc acctttggca gcatacaccc actctgccat ttcaggaatt cgcactttga
     1261 gcttatatcc ctgttcggca aatttttcct ccagccaacg gcaatataat cttgcccctt
     1321 cctgtgaaat gtttactacc ggataggcat tataagcaat attggtgtgg taattttcaa
     1381 ccataacatc catttgcatg ttgggtaata accatttatc agcagcaaca gcagcaatag
     1441 ccagggcttc agttttgccg tttttttcta aatcgtataa aaattctcta tatgcgagat
     1501 tgctcacttc cgattccaac atataaaatc cctgtataga aaccgttttg ccgtcaattg
     1561 tagttgtgcc ggatggaaca aattcaaaac ctcttttttt caggaatttc ttttcacttt
     1621 gtttaaaggt gaatgatgtt ccaaatgcga ttaataagcc acaaataaag gtaaggagta
     1681 gggttttgcg catgagctga ttgtttaaat aattaacgac aatttcagtg ttgttggtat
     1741 gtgatttcca aggcaaattt taatttatga ataagtaatt atcatacaat taatagtata
     1801 tattaaggtc cgaatttata aaatgcattt tttaattaat ttatttcaat tccatcaagg
     1861 ttggtaaaac cacaattgaa tataattctt atgtacaaaa aaaaagaggc tgccttttag
     1921 agacagcctc ttgaaaattg tttatttaca cgctggcact cactttaggc gcagcggata
     1981 atatttctgc atttgcttta tcagcatatt tctcgaaatt tttcacaaac tgtgcggcca
     2041 aattatttgc tttttgatcg taagcagttt tatcggacca ggtatttcga gggttgagta
     2101 tgtctgcagg aacattagga caagttgttg gcatcaacaa gccgaatatg tcatgtgcgg
     2161 taaaagtaac attatctaat tcaccattta aggctgcagt aatcatagcg cgggtataag
     2221 ataatttcat tctatgacca gtgccataag gacctgcagt ccagccggta ttaatgagcc
     2281 aaacattcac attgtgtttg cgcatttttt cacctaacat ttcagcatat ttggttggat
     2341 gtaaaggcaa aaacggtgca ccaaaacatg ttgagaaagt aagctgaggt tcagtaacac
     2401 ctgcttctgt tccggcaact ttagcggtat agccactcaa aaactgatac atggcttgtc
     2461 ccggagaaag tttactgatt ggaggcaaca ccccaaaagc atcacatgtt aaaaagaaga
     2521 tgttttttgg taaaccacct ttacttggtg tcatgatgtt atcgatataa ttgataggat
     2581 aactcacgcg tgtattttcc gttttggtaa tattcgcaaa atcgacatta cgtgtatttg
     2641 gatagaattc gatgttttcg agaatagcac caaattttat cgcattaaaa atctgcggtt
     2701 cgttttcttt agtcagatta acgcattttg cataacagcc accttcaaag ttgaaaacac
     2761 tgtcttctgt ccagccgtgt tcatcatctc cgattaaacg gcggttaggg tcggcagaac
     2821 gtgtagtttt tcc
//
LOCUS       DAORKW010000492         2817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2065_length_2817_cov_4.044895, whole genome shotgun sequence.
ACCESSION   DAORKW010000492 DAORKW010000000
VERSION     DAORKW010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2065_length_2817_cov_4.044895"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1969
                     /gene="nuoL"
                     /locus_tag="PLB46_14575"
     CDS             56..1969
                     /gene="nuoL"
                     /locus_tag="PLB46_14575"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRF77799.1"
                     /translation="MNSYNYISLIPILPFIGFLLLGLLGKRLPEKLSGFIGVLFIGIA
                     AVLSLTTAYQYFIVDGAINGVYPHLEPVKITWLQFTSTLSIDMGITLDPISVMMLVVV
                     TFISLMVHIFSLGYMHGEKRFATYYSHLQLFTFSMLGLVISSNLFQIYMFWELVGVSS
                     FLLIGYYYDKPSAVAASKKAFIVTRFADLFFLIGILIMGLTANSFDFNTIISTLTSEN
                     FRFGATFLGFSVLTWALVLIFIGGAGKSAMFPLHIWLPDAMEGPTPVSALIHAATMVV
                     AGVFLVARLFPVFAVTDSGALEIVAYVGIFSAAFAAVIACTQTDIKRVLAYSTMSQIG
                     YMMFALGVSDYEHGAGFTSSMFHLFTHAFFKSLLFLGAGAVIHAVHSNEMKDMGGLKK
                     YLPITHITFLIACLAIAGIPPFAGFFSKEGILHAAYESHPGIYYIALITSGITAFYMF
                     RLYYNIFWNKQDAHLHDFHGEGPISMKLPLIILGTLSIVVGFIPFAHYITPTGKPGEM
                     HLDLMFSIAPVLIGVVGIVLATLFYKSQNEKADKVATQLNGLYKAAYRKFYIDEVYLF
                     ITKKVIFNLIGRPAAWIDRNIVDGFMNALGNTTVSFSASLKDMQSGKVQEYALYFIAG
                     VIGLSLIGIYLWM"
     gene            2021..>2817
                     /locus_tag="PLB46_14580"
     CDS             2021..>2817
                     /locus_tag="PLB46_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRF77800.1"
                     /translation="MNLNLLIIIPAITALGIVFTSGLKQVRFVAFAGALIQLVLSFVL
                     LFLFQQERNAGNNEQFIFQATYNWFPSININYHIGVDGISIAMILLTAFVVLAGVLVS
                     WRQEKLSKEFFLLLTVLSAGAYGYFIALDLFTLFFFLEVAVIPKFLLIGIWGSGKKEY
                     SAMKLALMLMGGSALVFVGMIGVYFSGTNSLDLITNAQSSIAEETQRIFFPFLFVGFG
                     IFTALFPFHTWVPDGHSSAPTAASMFLAGISMKLGGYGCLRAAVYLMP"
BASE COUNT          773 a          464 c          550 g         1030 t
ORIGIN      
        1 aaccatcatt ctagtgatgt ggaagacaat gaagaaatga aatactaata acaacatgaa
       61 cagctataat tatatatcac tcattccgat tttacctttt ataggctttt tgctgttagg
      121 gttactcgga aaaagattac cggaaaaact ttcaggcttt attggagtgt tatttatagg
      181 aattgcagca gtattatcgc tcaccactgc ctatcagtat tttattgttg atggcgcaat
      241 aaatggtgtt tatccgcatc ttgagccggt taaaataacc tggttgcaat ttacatctac
      301 cttgtctatc gacatgggaa ttacgctgga tcctatttcc gtaatgatgc ttgttgttgt
      361 tacgttcatc tcattaatgg tgcatatttt tagtttaggg tatatgcacg gcgaaaaacg
      421 ttttgctaca tattattccc atttacaatt atttactttt agtatgttgg ggttagtaat
      481 atccagcaat ttgtttcaga tatacatgtt ttgggaatta gtgggcgttt cgtcattttt
      541 attaatcggt tattattacg ataaaccatc tgcagtagca gccagcaaaa aagcatttat
      601 tgtaacccgc tttgcagatt tatttttcct gattggtatt ttgataatgg gcttaactgc
      661 caactcattt gattttaata ccattatatc aacgctgaca tccgaaaact ttagatttgg
      721 agcaacattt ttaggcttta gcgttttaac ctgggcatta gtattaatat ttattggtgg
      781 agccggaaaa tctgctatgt tcccactgca catttggttg ccggatgcca tggaaggtcc
      841 aacacctgtt tctgcattaa ttcacgctgc taccatggtt gttgcaggtg tatttttagt
      901 tgcacgttta tttcctgttt ttgcagttac cgattcagga gcattggaaa ttgtagctta
      961 tgtaggtatt ttttccgccg cttttgccgc tgtaattgcc tgtacacaaa cagatattaa
     1021 acgagtattg gcatactcaa ccatgagtca aatcgggtat atgatgtttg cacttggtgt
     1081 tagtgattat gagcacggtg caggttttac gtccagtatg tttcatttgt ttacccacgc
     1141 attttttaaa tcattattat tcttaggagc aggtgctgtt atacatgcag tgcatagcaa
     1201 cgaaatgaaa gacatgggtg gattaaaaaa atatttacct attacccata tcacattttt
     1261 aatagcgtgt ttagctattg caggtattcc gccgtttgcg ggattcttta gtaaggaagg
     1321 cattttacat gctgcatacg aatcacatcc cggcatttat tatatcgcat taattacttc
     1381 cggtattact gcattttata tgttccgttt gtattacaat atattttgga acaaacaaga
     1441 tgcgcattta cacgattttc atggtgaagg tccgataagc atgaaattac ctttaatcat
     1501 tttaggtacc ttatcaattg tagttggatt tattccattt gcacactaca tcacaccaac
     1561 agggaaacct ggtgaaatgc atcttgattt gatgttttca attgcaccgg ttcttattgg
     1621 tgttgtcgga attgtattag cgaccttatt ttataaatca caaaatgaaa aagccgacaa
     1681 agttgctaca cagttaaatg gattatataa agcagcatat cgcaaatttt atattgatga
     1741 agtttaccta tttatcacta aaaaagtaat ttttaattta attggcagac ctgcagcctg
     1801 gatcgataga aatattgtgg atggtttcat gaatgccctt ggcaatacaa cagtatcttt
     1861 ttcagcttca cttaaggaca tgcagtcggg taaagtgcag gaatatgcat tatattttat
     1921 tgcaggtgtt attgggttga gtttgatagg tatttattta tggatgtaat tatttgtctc
     1981 caacaggtta caaataatga ttttattaag atgaagtaat atgaatttaa atttacttat
     2041 aataattcct gcaattaccg ctttggggat tgtgttcacc agtggattga aacaggtgcg
     2101 ttttgtcgca tttgccggtg ctttaattca gttggtgtta agttttgtgt tgttattttt
     2161 atttcaacag gagcgaaatg caggtaataa cgagcaattt attttccagg caacttataa
     2221 ctggttccca tctataaata tcaactacca tattggggtt gatggtattt caattgccat
     2281 gattttattg actgcatttg tagtattggc cggtgtatta gtttcatggc gccaggaaaa
     2341 actcagcaaa gaattctttt tattgcttac cgtattaagc gccggtgcat atggttattt
     2401 tattgcatta gatttattca ccttattctt cttccttgaa gttgcagtaa ttcctaaatt
     2461 tttattgata ggtatttggg gcagcggtaa aaaggaatat tcagctatga aactcgcttt
     2521 gatgttaatg ggtggaagtg ctttagtgtt tgtgggaatg attggcgtgt atttttcagg
     2581 cactaacagt ctggatttaa tcaccaatgc acaatcatca attgcagaag aaacacaacg
     2641 tatatttttc ccatttttat ttgtgggttt tggcatattc acagctttat ttccatttca
     2701 tacctgggtt cctgacggac actcatctgc acctactgca gcatccatgt ttttagcagg
     2761 tatttcgatg aaactaggtg gttatggttg cttacgcgct gctgtgtatt taatgcc
//
LOCUS       DAORKW010000493         2815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2068_length_2815_cov_5.507609, whole genome shotgun sequence.
ACCESSION   DAORKW010000493 DAORKW010000000
VERSION     DAORKW010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2068_length_2815_cov_5.507609"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..2557
                     /locus_tag="PLB46_14585"
     CDS             122..2557
                     /locus_tag="PLB46_14585"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRF77801.1"
                     /translation="MGKRFLGVILALFFYQQTQAQLLEPVKWEMSATQSGVNEYILIF
                     KATVDEGWKIYSKDVPELGPLPTVIVFENLPKGVELIGESEELGRMEEAVEPLFDNKL
                     IKYYHKRLTLRQKVRISSNVTLKGYIDYMSCDDKQCIALSKDFSFDLEGVANSNNYLG
                     IDYSKAKEPVKWEITTEKISDNAYWVKFNASISPTWKLYSQNSPKGGAQPMQFVFENA
                     GTNYHLEGKVEELGHVETKPEPLFENKVLKFYHDKAEMRQKIILDSANAVISGYIDYQ
                     TCDPSQCIYQNKDFNITISNGQVISTETPVDTAATGSVTFGDFKVDTAYANNAVCEGA
                     ATAQITNASGKSLFSTFILGFIGGLIGLLMPCTFPMIPFTISFFTKHAGNRRKGIMNA
                     SFYGFSIVLVYFLASLPFLFFGVGGDKLNDFSTNNVVNLVFFFVFILFALSLFGLFEI
                     KLPTKWANKADSASDVGGLLGIFFMALTLCIVSFSCTGPILGSLLANSLKDGGVALTM
                     GMLGFGTAFALAFTLLALFPQLLNKMPKSGGWLNEVKIVFAFIEVAMAVKFLSNVDLA
                     YHWDFLKREIFLGAWTLIALLITLYIAGVIRFPHDDKKVKWSGGRIVFALTFLVTTVY
                     FGQGLRKYGNVPMLSGFLPPQWYSIYEYDNECPLGLNCFHDLEEGIAYAKENNKPILI
                     DFTGYACVNCRKMEEHVWPKDEIFSIINEEYVLVSLYVDDKEALRLEEQYISATTGQK
                     VRTIGNKWSDLQSSQFGTNAQPYYVLIAPDGKVLNQPRAYTPDVDEYRGFLECGLETF
                     EQIDKLAMKRNAD"
BASE COUNT          874 a          425 c          567 g          949 t
ORIGIN      
        1 cactaacggc tacttccgca cgttaaagtt atatttacag tcactttttt gtcatgggac
       61 aggtgtatca tctgcgctca gtggtaaatc gttaaatttg caatctaaat tttattagca
      121 gatgggtaaa cgttttttag gggtcattct ggcgctgttt ttctatcaac agacacaagc
      181 acaattgctc gaaccggtta agtgggagat gtcggctacg caaagtggtg taaacgagta
      241 catcctgatt tttaaagcta cggttgatga aggatggaaa atttattcta aagatgttcc
      301 tgaactcgga cctttgccta ccgtaattgt gtttgaaaac cttcctaaag gagttgaact
      361 tattggggag tcggaagaat tggggcgtat ggaagaagca gttgaaccct tattcgacaa
      421 taaattaatt aaatactacc ataaacgttt aacacttcgt caaaaagtga gaatatcgag
      481 caatgtcact ttaaaaggat atatcgatta catgagttgt gacgataaac aatgtatagc
      541 tttaagtaag gatttttcgt ttgatttaga aggtgtcgcc aatagtaata attatttagg
      601 aattgattac tccaaagcaa aagaacccgt aaaatgggaa ataacaacag aaaaaatttc
      661 tgataacgct tattgggtaa aatttaatgc aagcatttct cccacatgga aattatattc
      721 tcaaaattcc cctaaaggtg gtgcacaacc aatgcaattt gttttcgaaa atgcgggaac
      781 taattatcac cttgaaggta aagttgaaga actaggtcat gtagaaacca aacctgagcc
      841 cttatttgaa aataaagtat taaagtttta tcatgataaa gcagagatgc gccaaaaaat
      901 aattctggat tctgctaatg ctgttatttc aggatatatc gattatcaaa catgtgaccc
      961 ttcacaatgt atttatcaaa ataaagattt taatataacg atatccaacg gacaagttat
     1021 ttcaactgaa acacccgttg atactgctgc aacaggttct gtaacatttg gcgattttaa
     1081 agttgatacc gcttatgcta acaacgccgt ttgcgaaggt gcagcaaccg ctcaaattac
     1141 taatgccagc ggtaaaagtt tattcagcac atttatttta ggttttattg gcggattaat
     1201 tggtttatta atgccatgta cttttccaat gattccattt acgatttcat tttttaccaa
     1261 acatgcaggt aatcgccgta aaggaattat gaatgcatcg ttttatgggt ttagtattgt
     1321 attggtttat tttttagcat cattaccatt tttatttttt ggtgttggtg gcgataaact
     1381 caatgatttt tctaccaata atgtggtgaa cctggttttc ttttttgtat ttattttatt
     1441 tgctttatcc ttattcggtt tatttgaaat taaattaccg actaaatggg caaacaaagc
     1501 cgacagcgcg tctgatgttg gtgggttatt aggaatattt tttatggcac ttacattgtg
     1561 tattgtgtcg ttcagttgca caggacctat tttaggttca ctactggcaa atagtttaaa
     1621 agatggcggc gttgcgttaa ccatgggtat gttaggtttt ggaactgcct ttgcacttgc
     1681 attcacattg ctcgcattat tcccgcaatt attaaataaa atgccaaaaa gtggtggttg
     1741 gttaaatgaa gtaaaaattg tttttgcatt tattgaagta gccatggccg ttaaattttt
     1801 aagtaatgtt gaccttgctt atcactggga ctttttaaaa cgtgaaatat ttttaggtgc
     1861 ttggacactg attgcattat taattacact gtatattgcc ggtgttattc gttttccaca
     1921 tgacgataaa aaagtgaaat ggtcgggagg gagaattgta tttgcactta catttttagt
     1981 gactacagtt tatttcggac aaggcttgcg taaatatggt aatgtgccta tgttgagtgg
     2041 atttttacct ccgcagtggt atagtattta tgagtatgat aatgaatgtc cgcttggttt
     2101 aaattgtttt cacgacctag aagaaggtat tgcttatgct aaagaaaaca acaagccgat
     2161 attgattgac tttaccggtt atgcctgtgt aaactgtcgc aaaatggaag aacatgtgtg
     2221 gccaaaagat gaaatattta gtattattaa tgaagaatat gtgttggtga gtttgtatgt
     2281 ggatgataaa gaagcattgc gtttagaaga acaatatatt tctgccacta ccggacaaaa
     2341 agtgcgcacc ataggtaata aatggagcga tttacaaagt agtcagttcg gcaccaatgc
     2401 gcagccttat tatgtattaa ttgcacctga tggaaaagtg ctcaatcaac cgcgtgctta
     2461 tacgcccgat gtggatgaat atcgtgggtt tttggaatgc gggttggaga cttttgagca
     2521 aattgataag ctggcgatga aaaggaacgc ggattgaacg gatttagcgg atttacgcgg
     2581 attaattttt ataagtaatt taattacttg ctctcatcta aatttatttg tatgtgttat
     2641 ttattaaaaa caatacatac taataaaaaa aaacgcagcc ggtaaatacc gaccacgttt
     2701 taataatttt tactttaatg atgtaattaa aatgttggtt tttcaggtag ggtataagtt
     2761 ctgccctgat tttgttgttt taaataatcc attaaaggca taaaataatc taacg
//
LOCUS       DAORKW010000494         2811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2074_length_2811_cov_4.649492, whole genome shotgun sequence.
ACCESSION   DAORKW010000494 DAORKW010000000
VERSION     DAORKW010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2074_length_2811_cov_4.649492"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PLB46_14590"
     CDS             complement(<1..697)
                     /locus_tag="PLB46_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77802.1"
                     /translation="MNKFFKRKLLSAMPFRLSKTETEMLNDMQIKDILSEPDTHKKNN
                     TGSNPYRVILDGFMGKVVRLMTIFSLLLLVFIIIGLVYKSLPVLGANSLKDLLFSSQW
                     SPFKGEFGFLPFILSTVYVTVIAAVISIPLSILTAIYLSEYANKRFLKVAAPMLDILA
                     GIPSVIYGIWGVLIIVPFIRNDVAGWFGVEDSSGYSLLAGGIVLSVMVFPVVVQIVYE
                     VLKIQFEQQREKPL"
     gene            complement(733..1725)
                     /locus_tag="PLB46_14595"
     CDS             complement(733..1725)
                     /locus_tag="PLB46_14595"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRF77803.1"
                     /translation="MRYFQLLVLVATTTILATSCSNDKSDKNTSSTELEGKITMSGAF
                     ALYPMAVQWGEEFKALHPKVQFDIQGGGAGKGMTDVLSGSTNLGMVSRDVNAEEISKG
                     AFDISVCKDAVLPTINAENPYKDLIYQKGITNQQFIDIWVTGKIVTWGDLLGNGSKEK
                     IEVYTRSDAAGAAESWAKYLGGKKQEDLIGVGVMGDPGLAQAVVKSKFAVGFNNVSFA
                     YDNVTKINNPGIAVLPIDINGNGTLEPEENFYNNMDDVNAAILSGKYPSPPARPLLFV
                     SKGAPTDPLVIAFLQYVLSEGQKGLSDAGYVTLPQDIIQQQLAKLPAAEVAEKK"
     gene            complement(1840..>2811)
                     /locus_tag="PLB46_14600"
     CDS             complement(1840..>2811)
                     /locus_tag="PLB46_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRF77804.1"
                     /translation="KLIMLPIMFGLIILHGSFTNPNEKPIKVETPAELEGKISLSGAF
                     ALYPLVVKWGEEFKKLHPKVQFDIQGGGAGKGMTDVLSNTVELGMVSREITPDEAKKG
                     AIGYAVAKDAVVATININNPYYAKIYEKGITKKQFYDIWVNRGVVSWGDLLKNGAKEK
                     MTVYTRSDACGAADAWAKYLGNKKQDDLEGVGVFGDPGIAKAVTADKFGIGYNNVAYA
                     YDFKTKKPNPGIGIVPIDVNENGKLDPEENFYSTSDKLNMAILAGKYPSPPARQLYLV
                     SNGTIDDKVTLAFLNWVLTDGQKYVAESGFVELPTEVIKEQIAKLPK"
BASE COUNT          877 a          587 c          418 g          929 t
ORIGIN      
        1 gtaatggttt ttcccgttgt tgttcaaatt gtattttaag tacttcatat acaatttgaa
       61 caacaacggg aaaaaccatt accgataata caattcctcc tgcaagtaaa gaatatccac
      121 ttgagtcttc aacaccaaac caacctgcaa catcattacg tataaaaggt acaataatta
      181 aaacacccca aataccataa ataacggatg gaattccagc cagaatatct aacattggtg
      241 cagcaacttt tagaaatctt ttgttggcat actcgctcaa ataaattgct gttaatatac
      301 taagaggaat actaatgaca gccgctataa cggtgacata aacagtactt aaaatgaatg
      361 gtaaaaaacc aaattcccct ttaaatgggc tccattgtga agaaaataat aaatctttta
      421 aactatttgc acctaataca ggcaatgact tataaacaag gccaattatt ataaatacaa
      481 gtaataaaag ggagaagatg gtcattagcc taaccacctt ccccataaac ccatctaaaa
      541 taaccctata cgggttcgaa cctgtattat tttttttgtg tgtgtcgggc tcagatagga
      601 tatctttaat ttgcatatca tttaacattt ccgtttcagt tttacttaac ctaaacggca
      661 tagcagatag caattttctt ttaaaaaatt tattcatacg tccgttatta gattatttag
      721 atggtataat atttattttt tttcagctac ttcagcagca ggtaatttgg ctaattgctg
      781 ttgaataata tcctgtggta atgtaacata acctgcatcc gaaagtcctt tttgaccttc
      841 agataataca tattgtaaaa atgcaattac cagcgggtct gtaggtgcac cttttgatac
      901 aaataataat ggacgagcag gaggtgaagg atatttgcct gataatatag cagcgttcac
      961 atcatccata ttattataaa aattttcttc cggttctaat gttccatttc cattgatgtc
     1021 aattggcaat acagcaatac cggggttatt tatttttgta acattatcat aagcaaaact
     1081 tacattatta aatccaactg caaatttgct tttaacaaca gcctgagcta aaccgggatc
     1141 acccattaca ccaacaccta ttaaatcttc ctgcttctta ccacctaaat attttgccca
     1201 tgattcggct gcacctgctg catcgcttct ggtatatact tcaatttttt ctttagagcc
     1261 attgcccaac aaatcgcccc atgttacaat tttaccggta acccagatat caataaattg
     1321 ctggttagtt atcccttttt gatagattaa atctttgtat ggattttccg cattaattgt
     1381 aggtaaaaca gcatccttgc aaacactaat atcaaacgca cctttgctga tttcttcagc
     1441 attcacatct cgcgaaacca tacctaaatt tgtgcttcct gaaagcacgt ctgtcatgcc
     1501 tttgccggct ccaccaccct gaatatcaaa ttgcaccttg ggatgtaaag ctttaaattc
     1561 ttcgccccat tgcacagcca ttggataaag tgcaaaagca ccactcatgg taatttttcc
     1621 ctctaactca gttgaggatg tatttttatc tgacttgtca tttgaacaag aagttgctaa
     1681 aatcgtggtt gtagcaacca acactagtaa ttggaaatat ctcatattat attttttgtg
     1741 caaatatatt ctacttattc gatatatcct atagttttga tagattagta cggaaaaaaa
     1801 tccatggagc ccaaggaggt gctccacgga aggtcatgat tatttaggaa gtttagcgat
     1861 ttgctccttg attacctcag ttggtaattc aacaaatcct gattcggcta catacttttg
     1921 tccatcggtt aatacccaat tcaaaaatgc cagtgttact ttgtcatcta tggttccatt
     1981 agaaaccaaa tacaattggc gggcaggtgg cgaaggatat tttccagcaa gaatggccat
     2041 atttaattta tcagaagtgc tgtaaaagtt ttcttccggg tccagtttac cattttcatt
     2101 aacatcgata ggcactatgc caataccggg atttggtttt ttagttttga agtcatatgc
     2161 gtaagcaaca ttattatatc ctataccaaa tttatcggca gttacagctt tagcaatacc
     2221 aggatcacca aatacaccta caccttctaa atcatcctgt tttttattgc ctaaatattt
     2281 agcccaggca tcagcagcac cacatgcatc acttctggta taaaccgtca ttttctcttt
     2341 tgccccattt tttaataaat cgccccaact cactacacca cggtttaccc aaatatcata
     2401 aaactgtttt ttggtaattc ctttttcata aatttttgca taataaggat tattgatatt
     2461 aatggttgcc actacagcat cttttgcaac agcataacct attgcacctt ttttcgcttc
     2521 atcaggagta atttctctac taaccattcc aagttccact gtgttactta aaacatcagt
     2581 catacctttt cctgctcctc caccttgaat atcaaactgc actttaggat gcaacttttt
     2641 aaattcttcg ccccacttta ccactaaagg gtataaagca aatgctccac ttaaagaaat
     2701 tttaccttcc aactcggcag gtgtttcaac ctttattggt ttttcattgg gattggtaaa
     2761 acttccgtgt aaaattatta gcccaaacat aatgggcaac atgataagtt t
//
LOCUS       DAORKW010000495         2809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2077_length_2809_cov_4.791213, whole genome shotgun sequence.
ACCESSION   DAORKW010000495 DAORKW010000000
VERSION     DAORKW010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2077_length_2809_cov_4.791213"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLB46_14605"
     CDS             <1..363
                     /locus_tag="PLB46_14605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRF77805.1"
                     /translation="NLNKYQYRGVSFVAWLLRIAANETAQYFRDNKKMRVVNVEQSGI
                     EAICDEDSSSAMEQRENVFKVLKQLDADSLELIEMRYFEKRSFKEIGAIKNITENNAK
                     LKVHRILEKIRKFLPVVQ"
     gene            370..2487
                     /locus_tag="PLB46_14610"
     CDS             370..2487
                     /locus_tag="PLB46_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77806.1"
                     /translation="MQKIKFKIAYKPLSTEQVNKHKDFDALMGMYAAAPQLNFFQKLF
                     KNKWIMFSSGIIIGGLIATVIALNSTDKSPDTIAQNNSSDNTQTITQTPTTVIEENQI
                     DEQTTVITEEPVINNASPVIEHSAEVETLAATTQSEQYEKVANSTTVANDIVANKKID
                     ETTNKNDLGLTPISNDVVLSNTEKPDVQNPEVDLNKNNITPNTTSISQRTLQQTEKIT
                     PFELQAYKFNVEQKAIEISIVKPGAVAELLLTQQQSATNNTTKNQTITQSPTNNSKTL
                     AQTPDTSVLVAKVSEVAALALKIEKKADALFEQITKSNQTKNDTTTIVSNNVIPDSVG
                     DNNEAKTIAPATFKNRYAQLSFVTPVSSNGIDGYKYYHHFSVNMIQGYNGAVQGLEVG
                     GVLNADKGYVIGGQFAGVGNVIGGDLTGMQGAGVFNFAKSTFGMQYSGVANYSKSGMR
                     GMQGAGIANYSGGQVDGMQAAGIINIASGTNYSGKLMQAAGVINIATSNDLLGLQASG
                     IVNVANNITGAQIGLINLAKNVKGTQIGLINIADTIDGVAIGLLSFSRNGIFDVEVYN
                     SDLFLANIALRFGSPYVYNTFAFGINPAADTMQYGYGFGIGGHIPVVNKFAVDVDGMI
                     WNTFEDNFDFNYDYVHLLNQFRVMPSYAITKYLSVYGGPVINVEVFDNDYTPFKENTF
                     ASYEGLNVTTGLSLGYVVGIRLF"
BASE COUNT         1021 a          470 c          517 g          801 t
ORIGIN      
        1 aatctgaaca aatatcaata ccggggagta tcatttgtag catggctgtt gcgaattgca
       61 gcaaacgaaa cagcccaata tttccgggat aataaaaaaa tgcgtgtagt aaatgtagaa
      121 caaagtggga ttgaagctat atgcgatgaa gacagcagtt cggcgatgga gcagcgggaa
      181 aatgttttta aggtcttgaa acaactcgat gccgattcgc ttgaactgat agagatgcga
      241 tactttgaaa aacgatcttt taaagaaata ggagcaataa aaaatatcac agaaaataac
      301 gccaaattaa aagtccatcg cattttagaa aaaatcagga aatttttacc cgtagttcaa
      361 taacagcaca tgcaaaaaat taaatttaaa atagcatata aaccacttag caccgaacag
      421 gtgaataagc ataaggattt tgatgcctta atggggatgt atgccgctgc accacaactc
      481 aattttttcc aaaaactttt taaaaacaaa tggattatgt tttccagcgg aataataata
      541 ggaggcctta ttgcaacagt aattgcatta aattccacag acaaatcacc agatacaatt
      601 gcacaaaata attcatccga taatacacaa accatcaccc aaacaccaac tacagtaatc
      661 gaagaaaatc aaattgatga acaaaccacg gtaataaccg aagaacctgt aatcaataat
      721 gccagcccgg taattgaaca tagtgctgag gttgaaacct tagcagcaac aacgcaatct
      781 gagcagtatg aaaaagtagc aaattcaact accgttgcaa atgatattgt tgcaaataaa
      841 aagattgatg aaacaacaaa taaaaatgat ctaggcctca ctcctatttc aaatgatgtt
      901 gtattaagta ataccgaaaa acctgatgtt caaaatcctg aggttgactt aaacaaaaat
      961 aacatcactc ccaatacaac atctatcagt caacgcacac tacagcaaac tgaaaaaatt
     1021 acaccatttg aattacaagc atacaaattt aacgttgaac aaaaagcaat tgaaatttcc
     1081 attgtcaaac ccggagcagt agcagaattg ttactaacac aacagcaaag tgctaccaac
     1141 aacacaacca aaaaccaaac tataacacaa tcgccaacaa ataacagtaa aactttagca
     1201 caaactcccg atacatctgt attagtggca aaagtgagtg aggtagctgc attagctttg
     1261 aaaattgaaa aaaaggctga tgctttgttt gaacaaatta ccaaatcaaa tcaaacaaaa
     1321 aatgatacaa caacaattgt ttcgaataat gtaatacctg attcagtagg tgacaataat
     1381 gaagctaaaa caattgcacc tgctaccttt aaaaatcggt atgcacaatt atcatttgtt
     1441 acacctgtaa gttccaatgg aatagatgga tataaatatt atcaccattt ttcggtaaac
     1501 atgattcagg gatataatgg agctgtgcag ggattagaag taggtggtgt attaaatgca
     1561 gataaagggt atgtaattgg aggccaattt gcaggtgttg gcaatgtgat tggaggagat
     1621 ttaaccggta tgcaaggtgc aggggtgttt aattttgcga aatctacatt tggcatgcaa
     1681 tattccggag tagcgaatta tagtaaatca ggtatgcgag gcatgcaagg agccggaatt
     1741 gccaattatt caggagggca agttgatggt atgcaagctg ccggaattat aaatattgca
     1801 tcaggcacaa attatagtgg taaattaatg caagctgccg gtgttatcaa tattgctact
     1861 tctaacgatt tattaggatt acaagcatca ggtatagtaa atgttgccaa caatattaca
     1921 ggtgcacaaa ttggtctgat taatttagcc aaaaatgtaa aaggcacaca aatcggatta
     1981 attaatattg ccgatactat tgacggtgtt gcaatcggtt tattgagttt tagtcgcaat
     2041 ggcatttttg atgtagaagt atataattcc gatttattcc ttgccaatat tgcacttcga
     2101 tttggaagcc cttatgttta taatactttc gcatttggaa taaatcctgc agcggatacc
     2161 atgcaatatg gatatggatt tggtattggc ggacatattc cggttgtaaa taaatttgcg
     2221 gtggatgtgg atggaatgat ttggaatact tttgaagata attttgattt caattatgac
     2281 tatgtgcatt tattaaatca attcagagta atgccaagtt atgcaattac caaatattta
     2341 tcggtttatg gtggaccagt tatcaatgtt gaagtatttg ataacgatta cacccctttc
     2401 aaagaaaaca catttgccag ctacgaagga ttaaatgtaa ccacaggttt atccctcggc
     2461 tacgtagttg gtataagact attttaagtg agttgaaata taatggttgt taactaacaa
     2521 gggctgtttg aaaatttcaa atagcccttg cctttttatt tcaaaacatc aaactaaaca
     2581 aattgattta ttatatcaac ttttgccctc tgtggttccg gctgttttag cccgcaattc
     2641 cgctggagcg gaacacaaaa aggtacacaa aagccacaaa atttaaacga taaaccatat
     2701 taagattaat tatttaatag catttttttt atgagataca acataaattg gtttttcaat
     2761 taatcttttg tgctctttgt gtgggttcag ttttgtgccc tacgtggtt
//
LOCUS       DAORKW010000496         2806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2082_length_2806_cov_4.911668, whole genome shotgun sequence.
ACCESSION   DAORKW010000496 DAORKW010000000
VERSION     DAORKW010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_2806_cov_4.911668"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="PLB46_14615"
     CDS             <1..1214
                     /locus_tag="PLB46_14615"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRF77807.1"
                     /translation="IRDRAREKGGRVVGLNDSGGARIQEGVNSLGGYADLFYRNTLAS
                     GVIPQISAIMGPCAGGAVYSPAITDFILMVEKTSYMFVTGPNVVKTVTHEDVSSEDLG
                     GAITHSSKSGVAHFATSNGLDCIMKIKQLLSYMPQNCEEDAPVLAYNGNNELRPNLNT
                     IIPENPNQPYDIREVITETVDDNSFLEVHQNYAENIVVGFARIGGRSIGIVANQPAIL
                     AGVLDIESSKKAARFVRFCDCFNIPLIVFEDVPGFLPGTDQEWRGIITNGAKLLYAFS
                     EATVPRITIITRKAYGGAYDVMNSKHIGADMNFAWPSAEIAVMGAQGAAEIIFKKEIE
                     DAANPQDKLKEKIDEYTTTFANPYRAANRGFVDEVIKPEETRIKIIRSLEMLKNKVAK
                     LPRKKHGNIPL"
     gene            1255..2346
                     /locus_tag="PLB46_14620"
     CDS             1255..2346
                     /locus_tag="PLB46_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRF77808.1"
                     /translation="MATKKKQSVIGEKEIDFLTKYLNNPSPTGYETEGQKIWLEYLKS
                     YTDDYFVDNYGSVAAIINPKASYKVVIEAHADEIAWSVHFITEDGFIYVERIGGSDHI
                     IAPSKRVNIHTAKGIKKAVFGWPAIHTRRVKDDTPELKNIFLDCGCTSKKEVLDLGIS
                     IGDPITFDDDFFVMNDKYFVGRALDNRIGGFIIAQVARLLKQNKIKLPYGLYIVNSVQ
                     EEIGLRGAEMMAQQIKPDVAIVTDVTHDSNTPMISKNLEGDLKLGRGPALTKGPAVHN
                     KLLKIIEDAAKANKIDWQRDVASRTTGTDTDAFAYSNGGVPTCLIALPLRYMHTTVEC
                     VHKQDVEAVIQLIYHTLLNLKPGFNYKYL"
     gene            complement(2546..>2806)
                     /locus_tag="PLB46_14625"
     CDS             complement(2546..>2806)
                     /locus_tag="PLB46_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AhpC/TSA family protein"
                     /protein_id="HRF77809.1"
                     /translation="YKSKGFNIFSVSLDTDKSKWLAAVQADGLVWDNHVCALDDANNK
                     AAIDYRITGIPMSFLVNKEGIIVAENLRGQALETKVMELINQ"
BASE COUNT          941 a          493 c          572 g          800 t
ORIGIN      
        1 gaattaggga ccgtgccagg gaaaagggcg gtcgggttgt aggcttaaac gactcgggtg
       61 gtgcacgtat tcaggaaggg gtgaactctt taggtggtta tgccgacctg ttttatagaa
      121 acaccctggc aagtggggta atacctcaga tttcagctat aatggggcca tgcgcaggag
      181 gagccgtgta ctcaccggct attaccgatt ttattctaat ggttgaaaaa accagttaca
      241 tgtttgtaac cggccctaac gttgttaaaa cagtcaccca cgaagacgtg tcaagtgaag
      301 accttggagg cgccataacg catagttcaa aaagtggagt tgcgcatttt gcaacaagca
      361 atggcttaga ctgtatcatg aagattaaac aattgttgag ttatatgccg caaaactgtg
      421 aagaggatgc tcctgttttg gcttataacg gcaataatga acttcgtccc aatttaaaca
      481 ctatcatacc tgaaaacccc aatcagccgt atgatatcag agaagtaatt acagaaacgg
      541 ttgatgataa ttcgttttta gaagtacatc agaattatgc tgaaaatatc gtagtaggat
      601 ttgcccgtat tggtggaaga agtattggca ttgtggcaaa tcaaccagca attttagcag
      661 gtgtgctcga tatcgaatcc agcaaaaaag cagctcgatt tgtgcgtttt tgcgattgtt
      721 ttaatatccc gcttatcgtt tttgaagatg tacccggatt tttacccggc actgatcagg
      781 aatggagagg tattattacg aatggtgcta aattattgta tgcatttagt gaagcaaccg
      841 taccgcgaat cacgattatt acccgtaaag catatggtgg agcatacgat gttatgaata
      901 gtaaacatat tggggcagat atgaatttcg cgtggccttc agcagaaatt gccgtaatgg
      961 gtgcacaagg tgctgcagaa ataattttca aaaaagaaat tgaggatgca gcaaatcctc
     1021 aggataaatt aaaagaaaaa attgacgaat acaccactac ttttgcaaac ccataccgag
     1081 ctgcaaacag aggatttgtt gatgaagtaa ttaaaccgga agaaaccaga ataaaaatta
     1141 tacgttcact cgaaatgctt aaaaataaag tggcaaaatt acctagaaaa aaacatggaa
     1201 atattccgct gtaaatgtaa ttagtgaaca aaatttgttg taaaaaaaat aaaaatggct
     1261 accaaaaaaa aacaatctgt aataggtgaa aaggaaattg atttcctgac taaatattta
     1321 aataatccct cgccaactgg ttatgaaacc gaaggacaaa aaatttggct ggagtattta
     1381 aaatcttata cagatgatta ttttgtggac aattatggca gtgttgctgc tattattaat
     1441 cccaaagcaa gttacaaggt agttattgaa gcgcatgccg atgaaatagc atggtcggtg
     1501 cattttatta cggaagatgg atttatatat gtagaacgta ttggcggttc tgaccatatt
     1561 attgcaccaa gcaaacgtgt taatatacat actgcaaaag gtattaaaaa agcagttttt
     1621 ggatggccgg ctatacatac tcgccgagtt aaagatgata caccggaact taaaaatatt
     1681 tttctcgact gtggatgcac ttctaaaaaa gaagtgctcg atttaggtat aagcataggt
     1741 gacccaatta ccttcgacga tgattttttt gttatgaacg acaaatattt tgttggcagg
     1801 gcactcgata acagaattgg aggatttatt attgcccaag ttgcaagact actcaaacaa
     1861 aataaaatta aattacctta cggcctttac attgttaatt ctgtgcagga agaaattggt
     1921 ttgcgaggtg ccgaaatgat ggcccaacaa attaaacctg atgtagcaat agtaacagat
     1981 gttacccacg attcaaatac acctatgatt tccaaaaatt tggagggtga tttaaaactt
     2041 ggtcgaggac cagcacttac taaaggaccc gcagtacaca ataaattatt gaaaattatt
     2101 gaagatgcgg caaaagcaaa taaaattgat tggcagcgag atgtagcttc acgaaccacc
     2161 ggtaccgata ccgatgcatt tgcgtattca aacggaggtg tgccaacttg tttaattgca
     2221 ttaccattgc gttatatgca tactacagtt gagtgtgtgc acaagcaaga tgttgaagcg
     2281 gttattcaac tcatttatca cactttactc aacctgaaac cagggtttaa ttataaatat
     2341 ttataaggtg actatcagag gaattgctgc tgtttattta ttatttgtca tactgcccat
     2401 aattgcagct gccattatta tttttatagc tgcgcgaaaa agaattaagt tacaaaagca
     2461 aacggacccc gattctttta aaaagaaaaa gccctcccgt ccgggaaggc ttcgaagatt
     2521 ttttacaatc aaaaaacaat aatagttatt ggttgattaa ttccatcacc tttgtttcta
     2581 acgcctgacc gcggaggttt tctgctacaa taataccttc tttatttact aaaaatgaca
     2641 ttggaatacc ggtaatacga taatcaattg cagctttgtt gttggcatca tctaatgcac
     2701 aaacatgatt gtcccaaact aacccgtcag cttgaacagc agccaaccat ttcgatttat
     2761 ctgtatctaa tgaaacactg aaaatattaa accctttcga cttata
//
LOCUS       DAORKW010000497         2797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2096_length_2797_cov_4.168855, whole genome shotgun sequence.
ACCESSION   DAORKW010000497 DAORKW010000000
VERSION     DAORKW010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2096_length_2797_cov_4.168855"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1181..2347
                     /locus_tag="PLB46_14630"
     CDS             1181..2347
                     /locus_tag="PLB46_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF77810.1"
                     /translation="MKNLLLILSLLIQFALSAQDVNFFTGTWEEANAKAKAENKMIMV
                     DAYTYWCGPCKVMDKTYFHNNQEVADVINKYFVPYKIECEHDFGIVFSRKFKINGYPS
                     LLYFNSEGQLVCRQMGYDNNQKTYIEGLYKIINSDQQDTYGYDATKLDMPWPDFYAND
                     FRNAEDSTWKRNKNVDPNGWLDQQSDLLAEMSWAIMSRFSLNEKYNTYFLDHYDEYHK
                     KYKLEADDKLTSVLYENVKKAAEQKNVDLMNTTEALYKKFLPNDEIGLHYLLSYYYEL
                     SNSWQAYANHFEIRMNNPANNVNINEINNIAWLIYENTDDKAVIEQTILWFKPYLETM
                     NGYNELDTYAALLTKVNRFKEAETWAIKAIEAGKSASMDVKATEELLETIKKNL"
     gene            2534..>2797
                     /locus_tag="PLB46_14635"
     CDS             2534..>2797
                     /locus_tag="PLB46_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRF77811.1"
                     /translation="MKTTFLIILVLFTVQASFAQKLPDFSFEDVNGNVINNQSLTANK
                     PVIVFYFDPFCESCLEQAALISKEITAFKDITLLWVTTNADPEN"
BASE COUNT          969 a          443 c          539 g          846 t
ORIGIN      
        1 tctgtggtct agttttaaac tgcatgatat gcggctcaaa ataaatccgg taatgctcag
       61 cccgacgcgt ttgacggagc gtatttaatt acaagatata aatcagaata tgatattgcg
      121 gaagtgccgg ggtttgttaa aaaagtgata ttcccaatca cagtggcaat cggcaaattg
      181 ctgggaaagt acaaacattt taaagatgcg cctgtgcctt taaaataaaa tggttgcata
      241 acgttttatg aattaaacaa ttggtgtttt ggttcaccaa ccttggctga tgcttcgcat
      301 tggttcatag gtacatttgc acattaatag tttcacgcgg aaaaagggaa aatggcggag
      361 tcgacaacag gctgtttaat tagcacatta gcacattatc aaatcagcac attaaatagt
      421 ctcgcgcaaa gctgcaaagg ttaagtttta tatgattggt tatttcaagc gcgctaagtc
      481 gttaaaccag gttcacccac ctcggcggac gtttcgcgca ttggtacatc ggtacattgg
      541 tacatttgca catttattag tttcacgcgg aaaaagggaa aatgtcggag ctgagaaccg
      601 gctgtttaat tagcacatta gcacataatc aaatcagcat attaataagt ctcgcgtaaa
      661 gcttataaac caatttcacg cggattaaat aaatgtgcgg ggagtagcgg agtttttgac
      721 tgataaacca aaaagccgaa accacggaga gaaaaataaa ggaaccagca cagataaaaa
      781 acaaaagttt gtcctccttg ggagctagct tttaaagcaa tcgggagtgt ctgtttgtta
      841 tgatgatggg tattacgctt tcagaattat acaatccgct ttgtataaaa caatttgaag
      901 agcgcaatac attattttat atattacttt ttccactttg gaaattaagg gcggtttact
      961 ttgttttaat gtaacatttt agtcattaat ttaggttttt tgatgagatt tttgaagatt
     1021 aattagtcat aaatgcttga atgtcaatct tatatgcata acaaaatgag gctattatta
     1081 gtatgataat ggaaagggta gattaatttt tcataccaat attagtagcg ttgccgttaa
     1141 tagttgctta ttttgaaatt taaaacctat attaaattaa atgaaaaacc tattattaat
     1201 cctatccctc ttaattcaat ttgcactatc cgcacaagat gtaaactttt ttaccggaac
     1261 ctgggaagaa gctaatgcta aagccaaagc agaaaataaa atgataatgg tagatgctta
     1321 cacttattgg tgcggtccgt gtaaggtaat ggataaaaca tattttcaca acaatcagga
     1381 agtggctgat gtaattaata aatattttgt cccttataaa attgaatgtg aacacgactt
     1441 cggtattgta ttttccagaa aatttaaaat aaacggctat ccgagcttat tatattttaa
     1501 ttcggaaggt caactggtct gcaggcaaat gggttatgac aacaaccaaa aaacttatat
     1561 tgagggatta tacaaaatca tcaattcgga ccagcaagat acttatggct atgatgctac
     1621 aaaattagat atgccatggc cggattttta tgcaaatgat tttcgtaatg ctgaagacag
     1681 cacttggaaa cgcaacaaaa atgttgaccc caatggttgg ttagatcagc agtcagattt
     1741 gttagcagaa atgagctggg caatcatgtc acgattttca ttaaatgaaa aatacaatac
     1801 ctactttttg gaccattatg atgaatacca caaaaagtat aaactggaag ccgatgataa
     1861 gttaaccagt gtattatacg aaaatgtaaa aaaagcggca gaacaaaaaa atgtcgattt
     1921 aatgaatact acagaagcgc tgtataaaaa gtttttgccg aatgatgaaa tagggttgca
     1981 ttacttgttg agttattact atgaattaag taacagctgg caggcatatg ccaaccattt
     2041 tgaaatacga atgaacaacc cggccaacaa tgttaacatt aatgaaatta acaatatagc
     2101 ttggttgata tatgaaaata cggacgataa agctgtaatc gaacaaacta tattgtggtt
     2161 taaaccctat ttggagacta tgaatggtta taacgaattg gatacctatg cggcactact
     2221 cacaaaagta aacagattta aagaagctga aacatgggca attaaagcta tagaggccgg
     2281 aaaatctgct tcgatggatg taaaagctac agaagaatta ttggaaacaa tcaagaaaaa
     2341 cctctgacag gtgcattccc tcaatagggt aaaggtaaac atggattaca catgaaaatt
     2401 gttgtgaaat tcggttaaaa ttgtttagtc ataagttgaa atactaattc aggcaagggc
     2461 gtttaccgtc cttgcttttt tgttgataat aatttatgat atatttggag acacaaatgt
     2521 ttaattgctt actatgaaaa caactttttt gataatatta gtattgttca ctgttcaggc
     2581 atcctttgct caaaaacttc ctgatttttc ttttgaagat gtaaatggaa atgtaataaa
     2641 caatcagtca ctgactgcca acaaaccggt aatcgttttt tatttcgacc ctttttgtga
     2701 atcatgcctt gaacaggcag ctttaatcag taaggaaatc actgcattta aggatatcac
     2761 tttgttatgg gtaacgacca acgccgaccc ggaaaac
//
LOCUS       DAORKW010000498         2762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2139_length_2762_cov_4.759143, whole genome shotgun sequence.
ACCESSION   DAORKW010000498 DAORKW010000000
VERSION     DAORKW010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2139_length_2762_cov_4.759143"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLB46_14640"
     CDS             complement(<1..683)
                     /locus_tag="PLB46_14640"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF77812.1"
                     /translation="MPIVFITGASSGFGKATAEKFAQAGYQLILNARRLDRLETLKTH
                     LEATYGSDVLLVPFDVRNREEVAQQLDKMPSAWQKIDVLINNAGLAAGRDAFQDGSMD
                     DWELMIDTNLKGLMYVSKWVVKGMMEQKSGHIINISSLAGQEVYPQGNLYCATKHAVT
                     ALSKGMRMDLLPYHIKVTNISPGLAETEFSIVRFKGDTEKASKVYEGYQPLQPEDIAD
                     VLFFAASRPA"
     gene            902..>2762
                     /locus_tag="PLB46_14645"
     CDS             902..>2762
                     /locus_tag="PLB46_14645"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF77813.1"
                     /translation="MKKIFTCLLFLVAIGNTFAQTTISGVVTDNKNNTLPGANIFLKD
                     TYDGTTSDLDGKYSFTTTETGKQIFTVSFVGYGNYEDTIELTSGALTINVELKELFNE
                     LNAVVITAGAFEASDEKKNTILKPLDIVTTAGADGDIYGALETLPGASKVGNDDGLYV
                     RGGSDYETKTVIDGMTVNNPYYSTLPDIPSRGRFQPFMFKGTVFSTGGYSAEYGQALS
                     SAVILNTEDMPEYSASGLSLAPIFVGGFHTQKFKNEKTALGGGINYTNLALFDEVYVP
                     TTFESLKSVEAVEGSLFFRQKTSQTGIIKLYAQKESSDFAVRVNDIDSLPLTDDISLG
                     NNYNYVNISYRELLTKNWGIQLGTSYSSNKDNIIADGLDIGRADQSAQGKVAFNNAIT
                     EKIKIRYGAEYQYQTYDQNAGDIAFNVKENYTAGFAESDIFITNDLAGRIGVRAENSE
                     VLGEINIAPRVSLAYKTGKNSQVSFAYGDFYQTPQPTYLYTTAVSPLTYEKATHYIGN
                     YQYVTNDRTFRVEGYYKDYDNLVTQESTDIPFVNNYFNDGKGYAKGIDVFFRDKKTIK
                     YADYWISYSYLDTKRKFLDYPEEVTPTFAAQHIFSLVYKHYFPTINTQVSVTYR"
BASE COUNT          884 a          540 c          490 g          848 t
ORIGIN      
        1 gccggacggg aagctgcgaa aaaaagcaca tcggcaatat cttcaggttg taagggctga
       61 taaccttcat acactttgga ggctttttcg gtatctcctt taaaacgtac tatactaaat
      121 tccgtttctg ctaatcccgg agaaatattg gtcactttaa tatggtaagg taataaatcc
      181 attcgcattc ccttacttaa tgccgtaact gcatgtttgg tggcacaata taaattacct
      241 tgaggatata cttcttgtcc cgctaaactg gaaatattaa tgatatgtcc ggacttttgt
      301 tccatcatgc cttttaccac ccatttgctc acatacatta agccctttaa attggtatca
      361 atcattaatt cccaatcgtc catgctccca tcctgaaatg catcgcgacc ggcggctaat
      421 cctgcgttgt taattaaaac atcaattttt tgccatgctg aaggcatttt atccagctgc
      481 tgagccactt cctccctatt acgcacatca aatggcacca acaatacatc actgccatac
      541 gttgcctcta agtgcgtttt aagtgtttcc agcctgtcta atcgtctggc attcaatatt
      601 aattggtaac cggcttgagc aaatttttca gctgttgctt ttccaaatcc ggaactcgca
      661 ccggtgatga atactattgg cataatcctg ttttggtgca attttaacta aaaacgcaca
      721 aacaaggcct ccgtttgtca accaaaagcc ttttgaagtg ttaaaacagt ttgtctttaa
      781 atttgatgca ttcatcgcca tttcgggctg aatcatccaa tcgattttga ttaaaactta
      841 aaatccatgt tactttgtac tgtaatttaa ccaaattgtc acattgatgt ttaaccagct
      901 tatgaaaaaa atatttacct gcttattatt ccttgttgct atcggtaata catttgcgca
      961 aaccactatt tcaggagtgg ttaccgacaa taaaaacaat acacttcccg gtgctaatat
     1021 ttttttaaaa gatacttatg atggcaccac cagcgatttg gatggcaaat acagctttac
     1081 caccactgaa accggaaaac aaatttttac ggtaagtttt gttggatatg gtaattatga
     1141 ggacactatt gaacttactt caggtgcgct gacaatcaat gtagaattaa aagaattatt
     1201 taatgaatta aatgcagtgg taattaccgc tggtgcattt gaagccagtg atgaaaaaaa
     1261 gaatacaatt ttaaaacctt tagatattgt taccacggca ggagccgatg gcgatattta
     1321 cggcgcattg gaaacacttc ccggtgccag caaagtaggt aatgatgatg gtttatacgt
     1381 tcgtggtggt tctgattatg aaaccaaaac ggtaatcgat gggatgactg tcaacaaccc
     1441 ctactactct acccttccgg acattccttc tcgcggacgt tttcagcctt ttatgtttaa
     1501 aggtaccgta tttagtaccg gtggttattc cgctgaatac ggacaagcac tttcatctgc
     1561 cgtaatatta aatacagaag atatgcctga atatagtgca tcaggtttaa gtcttgcgcc
     1621 aatttttgtg ggaggatttc acacccaaaa atttaaaaat gaaaaaactg cattaggtgg
     1681 cggtatcaat tataccaatc tcgctttatt tgatgaagtg tatgtaccaa caaccttcga
     1741 atcattaaaa agtgtggaag ccgttgaagg cagtttattt ttcagacaaa aaacttctca
     1801 aacaggtatt ataaaattat atgctcaaaa agaaagcagc gattttgctg ttcgcgtaaa
     1861 tgatattgat agtttaccat taacagatga tattagtttg ggcaataatt ataattatgt
     1921 caatatttca taccgcgaat tactaacaaa aaactggggt atccaactcg gcaccagcta
     1981 cagtagtaat aaagacaata ttatcgccga tggtttggat ataggccgcg ccgatcaatc
     2041 tgctcaaggc aaagtagcat ttaataatgc catcacagaa aaaattaaaa tccgttacgg
     2101 tgccgaatat caatatcaaa catacgatca aaacgccggt gatatagcat tcaatgttaa
     2161 agaaaattat actgcaggat ttgccgaatc tgatattttt atcacgaatg accttgcagg
     2221 aagaattggt gtacgtgccg aaaattctga agtattggga gaaataaata ttgcacctcg
     2281 tgtttcactg gcatataaaa ccggaaaaaa cagtcaggtt tcatttgctt atggcgattt
     2341 ttatcaaacg ccgcagccca cctatttata caccactgct gtttcaccac tcacgtatga
     2401 aaaggccaca cattatatcg gcaattacca atatgttact aatgacagga cattcagggt
     2461 agaaggttat tataaagatt acgacaattt agttacacag gaatcaaccg atattccatt
     2521 tgtgaataat tattttaatg atggtaaagg atacgcaaaa ggtatagatg tatttttcag
     2581 agataaaaaa acaattaaat atgcagatta ctggatttct tattcttatt tagatactaa
     2641 acggaaattt ttagactatc cggaagaagt tacaccaaca tttgcagcac aacatatttt
     2701 cagtttggta tacaaacatt atttcccaac cattaatacc caagttagcg tgacataccg
     2761 at
//
LOCUS       DAORKW010000499         2743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2168_length_2743_cov_5.052827, whole genome shotgun sequence.
ACCESSION   DAORKW010000499 DAORKW010000000
VERSION     DAORKW010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2168_length_2743_cov_5.052827"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PLB46_14650"
     CDS             <1..983
                     /locus_tag="PLB46_14650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRF77814.1"
                     /translation="FGMLLEGRNWEGGSEYRYGFNGMEIEVDVYGNETVIDFGARFYD
                     SKLGRWLSGDPLSGRHPGISPYATCNNNPILYIDSDGKDFYLNGNTEQAMDDIRGLVE
                     ETSRDNIIFTEIQPGLFKVEFSLEGGVINNDAGAQLLLNLTSASEIYLYTVSNRTDYM
                     KKNTKGEFEATWFDMTVDPTVKGEKYGEADGVANWSETAREVDSDNYNASLDKKSSFV
                     PVDQFGLDVNGNVALAETGKWLHPDKSEYTRSEMAFHELWENYERTTNKLPYAYPADM
                     NDKAVEKRAEWVGAHDMSIRAQTGKNSDRHSYAHTRDRNPAVAGRALYIE"
     gene            992..1513
                     /locus_tag="PLB46_14655"
     CDS             992..1513
                     /locus_tag="PLB46_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77815.1"
                     /translation="MKALLITISVLKLATVFGQINTDSIIDRKPSAILIAEANDLFIQ
                     LELFDTERKLIIEIENRSDQVLALAPQTYFSCFSYSSIDTIPQFVIDHNGPLKPDKSV
                     GIQFLYPGDAMQYYTLFWDCQNDMSGILADRLSYTLIYLKIEINQVDKFVMPTEVWQW
                     YSMASERLSISNF"
     gene            2087..>2743
                     /locus_tag="PLB46_14660"
     CDS             2087..>2743
                     /locus_tag="PLB46_14660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRF77816.1"
                     /translation="MLSNRKNKVSDRKAHDPFGMLLVGRNYSITNENYRFGFNGYENT
                     DELLGDNIAIDFGARVYDSRLGRFFSIDPRSGEYPWQTPFAYFSNSPIAIVDVAGMGG
                     PTYWIDEMADGGGESNFHTIKSGETLSEIAIKYQVSVDELVLWNNIEDKNKINSGDRL
                     YLNDPRPIEMVTESNGDSQSNSNETGSILTKGLASFGTFGTIGETSASTFRITNGVKG
                     N"
BASE COUNT          911 a          415 c          576 g          841 t
ORIGIN      
        1 cgttcgggat gttgttggaa ggacgaaatt gggaaggggg aagtgagtat agatatggct
       61 ttaacgggat ggaaatagaa gttgatgtat acggaaatga aacagtgatt gactttggag
      121 ctcggttcta tgattcgaaa ctgggtagat ggctaagtgg tgatccttta agtggaagac
      181 atcctggaat aagtccatat gcaacatgca ataataaccc gatactttac atagattctg
      241 atggaaaaga tttttattta aatggaaata cagagcaagc aatggatgat atccgaggat
      301 tagttgaaga aacgtcgcgc gataacataa tatttactga aattcagcct ggattgttta
      361 aagttgaatt tagtctcgaa ggaggtgtaa taaacaatga tgctggagca caattgcttt
      421 tgaacctcac ctcagcttca gaaatctatt tatacactgt ttcaaataga acggactaca
      481 tgaaaaagaa cacaaaggga gaatttgagg ccacttggtt tgatatgact gtagatccta
      541 cagtaaaagg tgagaaatac ggcgaggctg acggtgttgc aaattggagc gaaacagctc
      601 gggaggttga ttctgacaat tataacgcta gccttgataa gaaatcctca tttgtgccag
      661 ttgaccaatt tgggttggat gtcaacggta atgttgcgct tgctgagact ggtaaatggt
      721 tgcatccgga caaatcagaa tatactagat ctgaaatggc tttccatgaa ttatgggaaa
      781 attatgaaag gactacaaat aaactccctt atgcatatcc ggcagacatg aatgataagg
      841 ctgttgaaaa aagggccgag tgggttggtg ctcatgatat gtcgataaga gcccaaactg
      901 gaaaaaatag tgaccgccac tcctatgcgc atactagaga tcgaaatcct gcagttgcgg
      961 gaagagcctt atatattgaa tagtttcaac gatgaaagca ttattaataa ccattagtgt
     1021 ccttaagctt gctacggttt ttggtcaaat taatacagat agtattattg ataggaaacc
     1081 tagtgccata ttgatagcag aggcgaatga cttgtttatt caactagaat tatttgatac
     1141 cgaacgaaaa ttaattattg agattgaaaa taggtctgat caagttttag ccttggcacc
     1201 acaaacttat ttttcttgct tttcatattc aagtattgac actattcctc agtttgtgat
     1261 tgatcacaat ggccctttga agccagataa aagtgttggt atacaatttc tgtatccggg
     1321 ggatgcaatg cagtactata cgctattctg ggactgccag aatgacatgt ctgggatttt
     1381 agctgaccgc ttatcatata ctttaatata ccttaaaata gagattaatc aagttgacaa
     1441 atttgttatg ccaacagaag tgtggcaatg gtattccatg gccagtgaac gcctttcaat
     1501 aagcaacttc taaatatgca atttttcgaa atgtgacatc agctaacata gactgcaata
     1561 gagcgacgaa ctagataaaa aaactccttc tattagattt tacaccaatt atttgctact
     1621 tttagtatat tgcaagtcag gatacaatgt gttatgaaat tgtaattaac gaagataaat
     1681 tcgcatatat ttattatcaa aaatgcagca cagactggaa cactagttta ctttcaagtt
     1741 gggacttagc aaaggtatta aaacaatatt ttttggaaca aggtataaat attgaaatat
     1801 aaaatttgtg ttttaagctg gtaccacaaa aaacgagtta tagttatcct gtaaaaaaat
     1861 gtcaaatttt tattgcgtgg tttcatgtat ttattttcaa ctatttatac agctgtttaa
     1921 tgaatatcaa tattaatatt ttttgttata aacactacct cttgttaaga attttattgg
     1981 tggtgtatgc accacaaaat tttgacacct tttaattagg gaagcattag gactacataa
     2041 atcatacaat cttctacttt ttgcaaattt taagctactt ttaaacatgc tatccaacag
     2101 aaagaacaag gtttcagata gaaaagctca cgacccgttc gggatgttgt tggtgggtag
     2161 aaattattca attaccaatg aaaattatcg cttcggattc aatggatatg aaaacacaga
     2221 tgaattactt ggtgataata tcgcaattga ttttggtgca agggtttatg attcgagatt
     2281 gggtaggttt ttctccattg acccgcgaag tggtgaatac ccttggcaga ccccattcgc
     2341 ttatttttct aattcaccaa ttgctattgt tgatgttgct ggtatggggg gaccaacata
     2401 ttggattgat gaaatggcag atggtggtgg tgaatctaat tttcacacta taaagtctgg
     2461 agagacatta agtgaaattg cgataaaata tcaagtctct gtcgatgaat tagtgctttg
     2521 gaataacatc gaagataaaa ataagataaa ttctggtgat aggttatact tgaatgatcc
     2581 taggccaatc gaaatggtaa cagagagtaa tggagattca caatctaatt caaatgaaac
     2641 aggaagtatc ttaacaaaag ggctggcaag ttttggcaca tttggaacta taggcgagac
     2701 ttctgcaagc acatttcgga ttacaaatgg cgttaaaggt aat
//
LOCUS       DAORKW010000500         2740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2170_length_2740_cov_5.979888, whole genome shotgun sequence.
ACCESSION   DAORKW010000500 DAORKW010000000
VERSION     DAORKW010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2170_length_2740_cov_5.979888"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..>2740
                     /locus_tag="PLB46_14665"
     CDS             114..>2740
                     /locus_tag="PLB46_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77817.1"
                     /translation="MKRNTLGFILGLLLVFSVVASYILIRQTQRNLEITAAVERIEKE
                     YQQLENEIIATISDEVIISNLITDSYTFEVFSQLAAKPYGLIVYEQDSAIIWTNNFIT
                     PVKAQMVYNDAPLFISETNGEYIIFQHDFPGNIHVIGFLPLQFDFGIKNKYLNHVKTP
                     GIQIPEHLILQPRADENTVAVHAGNGNALFYISANPEVPPVPEYTHVLYILLGISLIL
                     LLIIVYFLAKWIVSKFNRLTGIVMLLVILVAIYWLIDSLLFQQVFGYLPLFDPQYYGS
                     PGIADSTGDLLIKTILLFCFALFFSNFFYVKLPATKSTISKVAILFIGVLLVALSLLT
                     GQIFRSLIMDSSISFNINNFFSLDYFSFVGFLCISILLLSYFFISIRCVEIMVRGNNN
                     KYQFALIVFGIGLLAIALQKLTGRAMQPDMVVLVTGFVLIRIFLLSKKIGTRSLGGMM
                     IWLIFFSSFSAYVFHTSITHKEDETKRLFALKKSIEKDPITEYLFSGIEASVVKQLAM
                     QGVTRDNEEKIRPGVINQIRQDLQSNYFKKYATDIFVFKNDTALISTNNEIVPTRYAF
                     IDAIENNGEFTSAENLYFINDYTGNYYYLAELAVRNDSGSQFMAYLRMVPKKFTGESI
                     YPELLIDDDLRTSASFVNFDYAIYNDHTLSEREGEYSYPLMDIFYMNKDVESLTVKQN
                     GYSHYIYKVTENKKVVVSIPEENWFQPISIFSYLFFFYLIFLVIILLLEWLSRVVQGN
                     LVFKEWLNASLQNKIQTSVISLIIFAFIMMGVATIYYIANQYNFSHKQRLLSKIEGVQ
                     TNIDVLFNDNRKKAGQDGIKSQQLKKIGNRVAELSEIHEMDINIYGPGGDLVASSQPD
                     IFDKGLVSKKMQPLAYFKM"
BASE COUNT          892 a          429 c          477 g          942 t
ORIGIN      
        1 atcaccaaaa atagtaaaat gatagataat atagcccgat tattgtatgt aagccaatgc
       61 caatataggg agaaaattaa attattggta cgaaataata aaattctaag ttgttgaaaa
      121 gaaatacttt aggctttatt ttaggcttat tactggtttt ttcggtagta gcctcctata
      181 ttttaattcg tcaaacacag cggaatttgg aaattaccgc agcagttgaa cgtattgaaa
      241 aggaatatca acagttagaa aatgaaatca ttgctaccat ttcagatgaa gttataatca
      301 gtaacctgat taccgatagt tatacattcg aggtgttttc tcagcttgct gccaaacctt
      361 atggtttaat tgtatatgaa caggatagtg cgattatatg gaccaataat tttattacgc
      421 cggttaaagc gcaaatggtg tataacgatg caccgctttt tatttcagaa acgaatggcg
      481 agtatattat ttttcaacac gacttccccg gcaatattca tgttattgga tttttacctc
      541 tgcagttcga tttcggcata aaaaataaat acctaaatca tgttaaaaca ccaggtattc
      601 aaatacctga gcatttaatt ttacaaccac gggcagatga aaatactgtt gctgtgcatg
      661 ccggaaatgg taatgcacta ttttatatat ctgcaaatcc tgaagtaccg cctgtaccgg
      721 aatacactca tgtattatat atcttattag gaatttcact tattctatta ttaataatag
      781 tgtactttct tgctaaatgg attgtaagta aatttaacag gctaacagga attgtgatgc
      841 ttttggttat ccttgttgct atttattggt taatcgattc ccttttattt caacaagttt
      901 ttggttattt gcctttattt gatcctcaat attatggttc accgggtatt gccgactcca
      961 ccggtgattt acttataaaa acaatattac ttttttgttt cgcattattc ttttctaact
     1021 ttttttatgt caaattaccg gcaacaaaaa gtacgatttc aaaagttgct atccttttca
     1081 ttggagtgtt attagttgcc ttgtcattgc ttacgggtca aattttcagg agtttgatta
     1141 tggactcttc catatcattt aatatcaaca actttttctc attagactat ttcagttttg
     1201 tagggttttt atgcatttct attttattgc taagttattt ttttattagc atcagatgtg
     1261 tggaaatcat ggttcgtgga aataataata aataccagtt tgcccttatc gtttttggaa
     1321 tcggcctatt ggcaattgcc cttcaaaagc ttacaggacg ggcaatgcaa cctgatatgg
     1381 tagtgctggt tacaggtttt gtgcttatca gaattttttt actcagcaaa aaaatcggga
     1441 caagaagttt gggtggaatg atgatttggt taatcttctt ttcatcattt agtgcttatg
     1501 tgtttcatac ttcaattacg cataaagaag acgaaactaa acgattattt gcattaaaaa
     1561 agtcgataga aaaagacccg attacagaat atttatttag tggaattgaa gcaagtgtgg
     1621 ttaaacaact tgccatgcaa ggggtaacac gcgacaatga agagaaaatt agacctggtg
     1681 tcatcaatca aattcgccaa gacctgcaat ctaattattt taaaaaatat gccaccgata
     1741 tatttgtatt taaaaatgac acggcattaa ttagcaccaa taacgaaata gtcccaaccc
     1801 gttatgcatt tattgatgct atagaaaaca acggagaatt cacctccgca gaaaatttgt
     1861 attttattaa tgactacaca ggtaattatt attatttggc agaattagct gtgcgcaacg
     1921 atagcggaag tcagtttatg gcttatttga ggatggtgcc taaaaaattt accggtgaaa
     1981 gtatttatcc tgaattattg attgatgatg atttaagaac atctgcaagt tttgtaaact
     2041 ttgactatgc tatatataat gaccatacac tttctgagcg ggaaggtgaa tattcctacc
     2101 cattaatgga tattttttac atgaataaag atgtagaaag tttaaccgta aaacaaaatg
     2161 gatacagtca ttatatttac aaggttacag aaaataaaaa agtggtagta tccataccag
     2221 aagaaaattg gtttcaaccc atttcgattt tctcttattt atttttcttt tatctaattt
     2281 tccttgttat tattttgttg ttggaatggt tgtcacgtgt agttcagggc aacctagtat
     2341 ttaaggaatg gctcaacgct tcacttcaaa ataaaataca aacatcagtt attagcttaa
     2401 taatatttgc atttataatg atgggggtgg caaccattta ttacattgcc aatcaatata
     2461 atttctccca caaacaacgg ttattgagca aaatagaagg agtgcaaacc aatatcgatg
     2521 tattgtttaa cgacaataga aaaaaagccg gacaggatgg aattaagtcg caacaattaa
     2581 aaaaaattgg taaccgtgta gctgaacttt ctgaaattca tgagatggat atcaatattt
     2641 atggtcccgg tggtgattta gtggcctcat cccaacccga tatttttgat aaaggacttg
     2701 tctctaaaaa aatgcaacca ttggcatatt tcaaaatgag
//
LOCUS       DAORKW010000501         2738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2174_length_2738_cov_3.099143, whole genome shotgun sequence.
ACCESSION   DAORKW010000501 DAORKW010000000
VERSION     DAORKW010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2174_length_2738_cov_3.099143"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PLB46_14670"
     CDS             complement(<1..486)
                     /locus_tag="PLB46_14670"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HRF77818.1"
                     /translation="MKKRLLFFSVMLMVISLGKAQWVIDSTNTAAVNMYAASTSTCAV
                     FSNGTEWNVFNAVTGVHTYGNLTIARTMIDVVSYGDKVYFAGGKYGYFADPQYTKTVN
                     VYNSVTNTWSTLNLSTAREIGGAGAIGNKVVFAGGTGRTDIAGPVYMYNKVDIFDAVT
                     GA"
     gene            complement(635..>1089)
                     /locus_tag="PLB46_14675"
     CDS             complement(635..>1089)
                     /locus_tag="PLB46_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77819.1"
                     /translation="YAILKIVREAKRDPKYEYPKNNNFLMPDLKYTGEFDEGYLEINY
                     LNLNGKYFVKNIFHAHTNSFTHVATNQEVYQITDYSEWTCDSIGYIIDDTLMQQLHNY
                     ENNEFVKYTYHPEQWEQLPPFFFVKQETVLNDIKKRGDLNILFLNGGK"
     assembly_gap    1090..1189
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1190..2076)
                     /locus_tag="PLB46_14680"
     CDS             complement(<1190..2076)
                     /locus_tag="PLB46_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77820.1"
                     /translation="MKYVVIIWLVLIGFCAPMQINAQHIYTATIINSATNSPMADCPI
                     QLEGVRGLYSTDSSGKFEINRPDTGAINLTVLGLNCHVAISIPKPNTQFSPVVVFCPP
                     YELETVPIEYLDGLKIMQKAAAQLPYLYADSAHIAFGFYRNYKKINHVFREITEANVA
                     TVMRIKNENNKLNCDEAFGLQKIRRTRFTIAIDQYYDRALHDVFKQNPVYHHEYSLLT
                     PYFLRHGDFYIDSTSTDSTWVISYSLRGITGENHGVDNYSSEDFYGEADESGYYIINK
                     DDYAILKIVREAKRDPKYE"
     gene            complement(2223..>2738)
                     /locus_tag="PLB46_14685"
     CDS             complement(2223..>2738)
                     /locus_tag="PLB46_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRF77821.1"
                     /translation="RGHLDNISNNMLIGALNYFNEKTDAVKNQLTGAYESVPKVQRDY
                     KAAGIGSIVVGDENYGEGSSREHAAMEPRFLGVRAILVKSFARIHETNLKKQGMLALT
                     FADKNDYDKILEDDVIDIIGLTSFAPNVPLTLVLNHSDGSSENITVNHTYNQQQIEWF
                     KAGSALNLINE"
BASE COUNT          838 a          508 c          435 g          857 t
ORIGIN      
        1 ggcaccggta acggcatcaa aaatatctac cttattatac atatacacag gaccggcaat
       61 gtcagttcgt cctgtaccac cggcaaaaac tactttattg ccaatagcac ctgctccgcc
      121 aatttctctt gcagtagata aattgagtgt agaccaggta ttagtaacgc tgttgtaaac
      181 atttactgtt ttggtgtatt gagggtcggc aaaataacca tatttaccac cggcaaaata
      241 tactttatcg ccataactca ctacatcaat catggtgcga gctattgtta aattaccata
      301 ggtatgtaca ccggttactg cattaaatac attccattca gtaccatttg aaaaaacggc
      361 acatgtagag gtggaagctg catacatatt gacagctgcc gtattggttg aatctattac
      421 ccattgggct ttgccaaggc taataaccat caacataaca ctaaagaata acaatctctt
      481 tttcataatt ttggttttta gaaacacatt tatataacaa agttctcccg aaaaccaagg
      541 gtgtaatgtg acaattgtta cttagcaggg tgacagataa tggtggtttc cagcaatttt
      601 ataggtatat ttttggagat taatcacgtg ggatttattt cccgccattt aaaaacaata
      661 tgtttaaatc accgcgtttt ttaatatcat ttaaaaccgt ttcctgcttt acaaagaaaa
      721 agggtggaag ctgttcccat tgctcgggat ggtaagtgta tttaacaaat tcgttgtttt
      781 catagttgtg taattgttgc ataagggtat catcaataat ataaccaata gaatcgcaag
      841 tccattccga ataatcggtt atttgataaa cttcctgatt ggttgccaca tgggtaaatg
      901 aattggtatg tgcatgaaaa atatttttta caaagtattt gccatttaag ttgagataat
      961 ttatttccaa atagccttca tcaaattcgc ccgtatattt taaatcaggc attaaaaaat
     1021 tattgttttt tggatattca tattttgggt cgcgtttggc ttcgcgtaca atttttaaaa
     1081 tggcataatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt attcatattt
     1201 tgggtcgcgt ttggcttcgc gtacaatttt taaaatggca taatcatctt tattaattat
     1261 gtaatatcca ctctcatcag cttctccata aaaatcttct gaggaataat tatctacacc
     1321 gtgattttca ccagtaatac cacgcaagct ataactaatt acccaggtgc tgtctgtaga
     1381 agtagagtcg atataaaaat caccatgtcg taaaaaatac ggtgtaagta aagagtattc
     1441 gtgatgataa accggatttt gtttgaaaac atcatgtaat gcacggtcgt aatattggtc
     1501 tatcgcaatt gtaaatcgcg tgcgtctgat tttttgtaaa ccaaatgctt catcgcaatt
     1561 taatttatta ttttcatttt taatacgcat caccgtagcc acatttgctt ccgtaatttc
     1621 acgaaataca tgattaatct ttttatagtt gcgataaaat ccaaatgcga tatgcgcaga
     1681 atcagcatat aaatagggga gttgtgctgc tgctttttgc attattttca acccatcgag
     1741 atattcaata ggcacagttt ccaattcata aggtggacaa aaaactacca ccggtgaaaa
     1801 ttgcgtattg ggtttaggaa tactaatagc aacatgacaa tttaatccaa gcacagtcaa
     1861 attgatggct ccggtatcag gtcggtttat ttcaaatttc cctgaactat cggtgctgta
     1921 aagtcctctc acaccttcca attgaatagg gcagtcagcc attggcgagt tagtagcaga
     1981 attaataata gtagcagtgt atatatgttg cgcattgatt tgcatgggtg cgcaaaaacc
     2041 aatcaatacc aaccaaataa tcaccacata tttcatatcc accaaaatta tacattacta
     2101 ttcattcaat ttcatcccaa agtaaaattt aaatacccct cccgtaactt cctgcctccc
     2161 cgccttcccg gcaaaaaaaa agcccccaca aaacgcggag gctccaaaaa aaatatcata
     2221 aatcattcat taattaaatt taacgcagaa cctgctttaa accattctat ttgttgttga
     2281 ttataagtat gattaacagt aatattttcg ctgctgccat cactgtgatt taacactaaa
     2341 gtaagcggca cattcggagc aaatgatgtt aaaccaataa tatcaattac atcatcttcc
     2401 aaaattttat cgtaatcatt tttatctgca aaagttaacg ctaacatacc ttgttttttc
     2461 aggttagttt cgtggatacg cgcaaacgat tttactaaaa tggcacgcac acctaagaaa
     2521 cgaggttcca tagcagcatg ttcgcggctt gaaccttcac cgtaattttc atcaccaact
     2581 acaattgaac caattcctgc agctttataa tcgcgttgca cttttggtac tgattcgtat
     2641 gcacctgtta attgattttt aacggcatcc gttttttcat taaaataatt gagtgcacca
     2701 attaacatat tattggaaat gttatctaaa tgtccgcg
//
LOCUS       DAORKW010000502         2735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2179_length_2735_cov_4.389552, whole genome shotgun sequence.
ACCESSION   DAORKW010000502 DAORKW010000000
VERSION     DAORKW010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2179_length_2735_cov_4.389552"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1742)
                     /locus_tag="PLB46_14690"
     CDS             complement(249..1742)
                     /locus_tag="PLB46_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRF77822.1"
                     /translation="MTKSNKLTAFILVAMALGILIGGILNKKATGKELVYTPLADSLG
                     RDFITLVYDDNTFTQEVFIAKDDKEKKIMADSLGNAVAVVVKGKSSVFIPACIQTGKV
                     GDLNCTIEPSGKGVVEKNLIKKIAANVSVLTTIFLLLIKMIIAPLVLSTLIVGIAKMG
                     DTKTLGRVGGKTMLWFITASLVSLVLGMVLVNAFQPGEHMNLTLPAENAKTGVEGTAI
                     TFVSFITHVFPSSIFKAMAENEILQIVVFSIFFGVAAAAIGEKAMPLVKALDTLSHIM
                     IKITIYVMGFAPYAVFAAMCAVITTKGLSVLSTYAIFMGEFYFGLLILWVILISAGFA
                     VLRKRVFILLRRIKEPFLLAFSTASSEAAFPKLITELERFGCNNKIVSFTLPLGYSFN
                     LDGSMMYMTFASIFIAQCYGIDLTISQEITMLLTLMITSKGIAAVPRASLVVIAGTIS
                     MFNIPQAGLVLLLGIDTFLDMGRSATNVIGNAIATAAVSKWEGALED"
     gene            1877..2515
                     /locus_tag="PLB46_14695"
     CDS             1877..2515
                     /locus_tag="PLB46_14695"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRF77823.1"
                     /translation="MKKLNSFQIGTIVTGIIVVVAIGFVYNNTLKTHKEPVLPYFGPE
                     QGDGQPHFVSSFNLLNQDSVMLNQEPYKGKIYVADFFFTTCLGICPLMTEGMIRVHKA
                     YLNNPDIMILSHTVDPDHDTPSVLKRYANNRKIDTNKWNFVTGDKKQIYDLARYSYLV
                     DASEGDGGPNDFVHTENFALVDREGRIRGYYDGTDTAEVSKLIRDISLLLAE"
     gene            2589..>2735
                     /locus_tag="PLB46_14700"
     CDS             2589..>2735
                     /locus_tag="PLB46_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRF77824.1"
                     /translation="MITVNNVSLRYGKRILFDEVNLKFTKGNCYGIIGANGAGKSTFM
                     KIISG"
BASE COUNT          876 a          572 c          456 g          831 t
ORIGIN      
        1 ctgcatcatc caattggtat ccactaacgc ggacgcttcg cgcatcggta cattctcaca
       61 tcggtgcatc aattagcaca tcagcacatt accccatcag cacattaatt ggtgcattcc
      121 cacattagta catttcccaa tgggtacatc gctacattct cacatcatta aatcccccaa
      181 ttggtacata ggtacattct cccattggta catcaattag cacatcaccc catcagcaca
      241 ttagcacatc aatcctccaa agccccttcc cacttactca ccgcagcagt tgcaattgcg
      301 ttaccaatta cgttagttgc acttctaccc atgtcgagga aagtgtcgat tcctaataat
      361 aacactaaac cggcttgagg aatattaaac atgctaatgg tgccggcaat aactaccaaa
      421 ctcgctctcg gaactgcagc aataccttta gaagtaatca ttaaagtgag taacatcgtt
      481 atttcttgcg aaatggttaa atcaattcca taacactgcg caataaaaat ggatgcaaaa
      541 gtcatataca tcatactacc atctaaatta aatgagtagc ccaatggtaa agtaaaactt
      601 acaattttat tattacagcc aaatcgttct aattcggtaa ttaatttggg aaatgccgct
      661 tcactacttg cggtactaaa tgccaataaa aatggttctt taattcttcg caataaaata
      721 aaaacgcgtt tgcgtaacac agcaaaacca gcactaatta aaatgaccca taaaattaac
      781 agaccaaaat aaaattcgcc cataaaaata gcataggtag aaagtaccga caatcctttt
      841 gtggtgataa ctgcgcacat tgcagcaaaa acagcataag gtgcaaatcc cattacataa
      901 atagtaattt taatcatgat atgcgaaagt gtatccaaag cttttaccag tggcattgct
      961 ttttcaccaa ttgcagctgc agcaactcca aaaaatatac tgaacactac aatttgtaat
     1021 atttcatttt ccgccattgc tttaaaaata ctagaaggaa aaacatgtgt aataaatgaa
     1081 acaaatgtga tggcagttcc ttcaacacct gtttttgcat tttcggcagg cagggttaaa
     1141 ttcatatgct caccgggttg aaatgcattc actaatacca tccctaatac gagtgatacc
     1201 aaacttgctg taataaacca caacattgtt ttcccgccaa ctcttcccag tgtttttgta
     1261 tcacccattt ttgcaatacc tacaattaat gtagataata ctagcggtgc aattatcatt
     1321 ttaattaata ataaaaatat tgtggtaagt accgaaacat tggcagcaat ttttttaatg
     1381 aggttttttt cgacaacacc tttaccgctt ggttcgatag tacaatttaa atctccaact
     1441 tttccggttt gaatacatgc cggaataaat accgatgatt ttccttttac cacaacagct
     1501 acagcattac ccaaactatc agccattatt tttttctcct tatcatcttt agcaataaaa
     1561 acttcctgcg taaatgtatt atcatcgtaa accaaagtaa taaaatcacg gcccagcgaa
     1621 tcagctaaag gggtatacac caattctttt ccggtagctt ttttattcaa aattccaccg
     1681 attaaaatgc cgagtgccat ggcaactaaa ataaatgcag tgagtttgtt cgactttgtc
     1741 atgcctaaaa ttttgcccta agatatatgt ttccggacac ttactgttgc tattaagttt
     1801 gcccgccgca aattgtgtga atttatttta aatggctttg tttatcgtaa taaactccgg
     1861 taattttgca tcatatatga aaaagttaaa tagttttcag attggcacta tcgtaaccgg
     1921 aattattgtt gtagtcgcca taggattcgt ttacaacaat acgctaaaaa cacataaaga
     1981 accggttctc ccttatttcg gaccagaaca aggcgatgga cagccgcatt ttgtctcctc
     2041 attcaatctg cttaatcagg acagtgtgat gctcaaccag gaaccctata aaggcaaaat
     2101 ttatgtggcg gacttctttt ttaccacttg tttaggtatt tgtccgttaa tgactgaagg
     2161 aatgattcgt gtgcacaaag cttatctgaa taatccggat atcatgattc tttcgcatac
     2221 ggtggatcct gaccatgata caccctccgt tttgaaacgt tatgccaata atcggaaaat
     2281 tgatacgaat aaatggaatt ttgtgacagg cgataaaaaa caaatctatg acctggcgcg
     2341 ctacagctat ttggtggatg cgtcggaagg tgatggagga ccaaatgatt ttgtacatac
     2401 cgaaaatttt gctttggtgg accgggaagg cagaattaga gggtattacg acggaaccga
     2461 taccgctgaa gtgagcaagt taattcgtga catcagttta ttgcttgctg aataggcata
     2521 ttaccagtta aattatgctg tttttccctt aatttctgct acctttgcag ccgcaaattg
     2581 attaggcaat gattactgtt aataatgttt ctctccgcta cggcaaacga attctttttg
     2641 atgaagtaaa tttaaaattt accaaaggca attgttatgg tataattggt gctaatggtg
     2701 ccggtaaatc taccttcatg aaaataattt ccggt
//
LOCUS       DAORKW010000503         2714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2203_length_2714_cov_5.203460, whole genome shotgun sequence.
ACCESSION   DAORKW010000503 DAORKW010000000
VERSION     DAORKW010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2203_length_2714_cov_5.203460"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1275)
                     /locus_tag="PLB46_14705"
     CDS             complement(139..1275)
                     /locus_tag="PLB46_14705"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRF77825.1"
                     /translation="MNKTITFKPLYHKGSNQIGLYFEYNTSLIEKIKQLGCRFSKSNR
                     CWYIFYTEKNFNLLFNTLKSYAWIDYSAFKKNNLEIDIVTTSKKEIKTEFVFSEILDE
                     QFNLFKRWMEHRRYSKQTITTYIDCIKVFFKYHNNIEPVAINKHHIVDFNTNYILAKE
                     LSVSYQNQFINALKLFLIEVCQTILPIEALERPRREKRLPDVLSKEAIKSIINATKND
                     KHQLMLSIIYACGLRAGELINLRLTDIDFKRKVINIVQSKGCKDRRVPLPDGIAALLP
                     PYLQRHKPISWVFEGQQAGLQYTYKSLQMVFKNCVILAKVNKAATLHWLRHSYATHLL
                     EAGVDLRYIQELLGHSSSKTTEIYTHVSNTRLQQIPSPYEDLFK"
     gene            2033..2596
                     /locus_tag="PLB46_14710"
     CDS             2033..2596
                     /locus_tag="PLB46_14710"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRF77826.1"
                     /translation="MLLVPIIIIVVLLLIIVGLYNTLVGRKNQVENAFGTIDAMLKKR
                     FDLIPNLVETVKQYMTYEQDTLTKIVELRNSIQAQPNMPAQERIGMENQLTGLLSGLR
                     VAVENYPDLKANTSFIQLQSTWTEIEEQISAARRTYNAAVTAYNNSVEMFPTNIMANM
                     MGYKRKEVLAIEEAERKNVSAKDLFNK"
BASE COUNT          850 a          455 c          476 g          933 t
ORIGIN      
        1 caaaaagaac ccgattttgt ctgacgggtt gtgtcgtcat agccttgcca ctaacttgtt
       61 tatatgcgca actttacttc gccaagcatg ccaatttggg tgtataagcg aagtaatata
      121 tgttgcaata taatatgttt atttgaataa atcttcatag ggtgatggta tttgttgaag
      181 ccgggtgtta ctaacatggg tgtatatttc tgtggttttg ctgctgctat gtcctagtaa
      241 ttcctgtata taccgcaagt ctacaccggc ttctaataga tgtgtggcgt aagaatgacg
      301 caaccaatgt aatgttgctg ctttatttac ctttgctaaa attacgcagt ttttaaaaac
      361 catttgcaag cttttgtacg tgtattggag ccctgcttgt tgaccctcaa agacccagga
      421 tattggctta tgtctttgta aatagggcgg caataaagct gctattccat ccggtaaagg
      481 aactcggcgg tccttacagc cttttgattg aacaatattg attacctttc ttttaaagtc
      541 aatatcggtt aaacgtaagt ttattagttc accagctcgc aacccacacg cataaattat
      601 acttaacatt aattgatgct tgtcattttt tgtggcattt attattgatt ttatagcttc
      661 tttactcaat acatcaggta accgcttttc ccgccttggt ctttctagcg cttctattgg
      721 taatatggtt tgacacactt caatcaaaaa taattttaat gcatttataa attgattttg
      781 ataagatacc gataattctt ttgctaaaat ataatttgtg ttaaaatcta cgatatggtg
      841 tttattgatt gctacgggtt caatattgtt gtggtatttg aaaaaaactt taatacaatc
      901 aatatatgta gtaatagttt gtttgctata tcttcggtgc tccatccatc gtttaaatag
      961 attaaattgt tcatctaaaa tttccgaaaa aacaaattcg gttttaattt ctttttttga
     1021 agttgtaact atatctattt caaggttgtt ttttttaaaa gcactataat caatccatgc
     1081 ataacttttt agggtgttaa ataataaatt aaaatttttt tcagtataaa aaatgtacca
     1141 acatcgattg cttttactga acctacatcc taattgtttt attttttcaa ttaaggatgt
     1201 attatactca aaatacaaac caatttgatt agagccttta tgatataaag gcttaaaagt
     1261 gattgtttta ttcatttgtt ttaagggttt attagcacaa atgtattgat atgaaatata
     1321 aatgccaaaa ataaagtata cacttcggat gtgtattggt tgaaaaaaag aacatcttta
     1381 atgcaagcaa aattaaatag cctccttttt taaaaaatcc ttaaattgtt atgctttcaa
     1441 agaataaaat gaatagtttt atttagcacc ctttctgaat agttttttct gatattgtta
     1501 accgcttaac cattcttgta cgttaactgt aagttgaatg gcaacaaatt ttgcgactaa
     1561 aaaaaattaa cattggccaa gatattttcc attgccctga cggattcaat tcggggaagg
     1621 tacatcatat taaatttgta gatttatcaa tactgcttgc tatcgtgaga acaataattt
     1681 aattccgtaa tgaagtgaaa attatttttt gatcgtaatg aataaattga aaatttgcaa
     1741 ccttaagtat aaatctcaac cttattcaat ccctaaattt tgctcaattc agcaattatt
     1801 tataacaaca ttttcaaaaa cagtactact gtcaacatag ccccgatagt agcgaaaacc
     1861 ccgcagccga ggaacgagga gaggagttgc agcgaatagc gggaaccgaa ttttagggcg
     1921 aagcggaatt ttgctgcttc aaatcacttt tttgctcaaa ccgtggtttg cagttagaat
     1981 atatctttcg cttattcgtc atcttattat ctttacccga aatatcacag ccatgttact
     2041 tgtacctatt attatcatcg ttgtattgtt gctcattatt gtgggtttat acaacaccct
     2101 tgttggtcgc aaaaaccaag tagaaaacgc ctttggaact atcgatgcta tgcttaaaaa
     2161 gcgcttcgat ttaattccaa acctggtgga aaccgttaag cagtatatga cctacgagca
     2221 ggataccctg accaagattg tggaactgcg caacagcatt caggctcagc caaatatgcc
     2281 tgctcaggag cgtatcggaa tggaaaatca gcttaccggc ttgttgagcg gtttgagggt
     2341 tgcggttgaa aattatccgg atttaaaggc aaataccagc tttattcaat tgcaaagcac
     2401 ctggacagag atagaggagc agatttcggc tgcgcgtcgc acctataatg cggctgtaac
     2461 tgcttataat aactcggtgg aaatgtttcc aaccaatatt atggccaata tgatgggtta
     2521 caaacgcaaa gaagtgcttg ccattgaaga ggctgagcgc aaaaatgtat cggcaaaaga
     2581 cttgtttaac aagtaataac aaactttttt ataccgattt cgttatataa tttcgtaaaa
     2641 caactaaaat aaaagataga aaatgatagg agcttacatt attggtattg tgttcgccat
     2701 aatcggattg attg
//
LOCUS       DAORKW010000504         2714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2204_length_2714_cov_4.701015, whole genome shotgun sequence.
ACCESSION   DAORKW010000504 DAORKW010000000
VERSION     DAORKW010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2204_length_2714_cov_4.701015"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLB46_14715"
     CDS             complement(<1..613)
                     /locus_tag="PLB46_14715"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRF77827.1"
                     /translation="MTLVNVLIIGAGRSGTTSVSAYLAAHPEVSSSITKELHYFSIAD
                     LYKRGESYFHSLFPNETKKITTTADTYLLMDHAAPEKVKAYNPNMKIIIMLRDPISRS
                     YSNYNYSVNFGHEKKENTFLSTIDLEKERLNTLDIVDVNNTCHLYGSLYHKHISIWNK
                     YFDASQLVILKLNDLKNNPEAFYRNLCNRLDINYVPFEEANKEF"
     gene            complement(610..1596)
                     /locus_tag="PLB46_14720"
     CDS             complement(610..1596)
                     /locus_tag="PLB46_14720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRF77828.1"
                     /translation="MKQLYFNTFNNRNKNADLQRLAKNTGVHIIFPFYHIVSNQSVPH
                     IKHLYPVKDTIAFEDEMRFLTQHFTPLHLDDYVAGNYEKGKMYFVLSFDDGLSEMHSV
                     VAPILKQMGIPATFFLNSDFIDNKGLFYRYKVAVLIEKVATDELSKQITNSIGLGKVK
                     IANPAKYLLQLKWSDTELIDNIALACNVSFNDYLAELTPYLTSAQIQVLINEGHAIGA
                     HSINHPEYRTIDPQIQFLQTRDSMQIIADNFGLKKKYFAFPFTADGVVKELFPAMYGE
                     LNIDLSFGTAGLQTTKYPKHIERIPAEHVRYRLQEIIGNEYYYYRFKRRLGK"
     gene            complement(1681..>2714)
                     /locus_tag="PLB46_14725"
     CDS             complement(1681..>2714)
                     /locus_tag="PLB46_14725"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HRF77829.1"
                     /translation="INPFNPFNNANLTQEHFEGLDEALPEPRETQYFIEQPKKILNKL
                     EMPDAAMMYSVNPYQGCEHGCIYCYARNSHNYWGFSAGLDFETKIIVKHNAAELLEKV
                     FMEQKWVPGPVTFSGNTDCYQPVERKLGITRSLLEVCRKFKNPAGIITKNQLILRDID
                     ILKDLAKENLIFVNITITTLKEDLRRQLEPRTSSCGNRLKTIEKLAEAGIPVGVMIGP
                     IIPGLTDSEIPDIMNAAADAGAQTANYTMVRLNGALDKIFTDWIWKTYPMKAEKVLNN
                     IRSTHQGQLNDSRWSLRMRGDGELTNSIHQLFYLMRDKYMPKKKAFEYNIKSFKRPGQ
                     LKLDFDLFA"
BASE COUNT          849 a          505 c          456 g          904 t
ORIGIN      
        1 taaattcctt attggcttct tcaaaaggga cataattaat atccaatcga ttgcacaagt
       61 tacgataaaa tgcttccggg ttgtttttta aatcatttaa tttaagtata acaagctgag
      121 aagcatcaaa gtatttattc caaatgctga tatgtttatg atacaaactg ccatataaat
      181 gacaagtatt atttacatca acaatatcga gtgtatttaa tctttctttt tccaaatcaa
      241 ttgtactcag aaacgtattt tcttttttct catgaccaaa attgactgaa tagttataat
      301 tactatacga acgtgaaatt gggtcgcgca gcataataat tattttcata ttaggattat
      361 acgctttcac cttttccggt gcagcatggt ccattagcaa ataggtgtcc gctgttgttg
      421 taattttttt tgtttcattc ggaaacaaac tatgaaaata ggattcacct cttttatata
      481 aatcggcaat agaaaaataa tgtaactctt ttgtaataga gctactcact tcaggatgcg
      541 ctgcgagata agcagaaaca gaagttgtac cgcttctacc tgcgccaata atcaacacat
      601 ttaccagcgt catttgccta gcctcctttt aaagcggtaa taataatatt cgttgccaat
      661 tatttcctgc aatctgtaac gtacatgttc agccggaata cgttcaatat gttttggata
      721 tttggttgtt tgtaatccag ccgtaccaaa actcaagtca atgtttaatt ctccatacat
      781 ggctggaaat aattctttta caacaccatc ggcggtaaaa ggaaaagcaa aatatttctt
      841 tttaagcccg aaattatctg caattatttg catactatcc cgggtttgca aaaattgaat
      901 ttgtgggtcg atagttcgat attcaggatg atttattgaa tgtgcaccaa tggcatgacc
      961 ttcattaatt aaaacctgaa tttgagcaga agttaaataa ggagttaact cagctaaata
     1021 atcattgaat gatacattac aagccaaagc aatgttatca attaattcag tatcgctcca
     1081 tttaagttgt aataaatatt ttgcgggatt tgcaattttt acttttccta aacctattga
     1141 attagttatt tgtttgctca attcgtctgt cgcaactttt tcaattaaaa cagcaacttt
     1201 atatctataa aacaatcctt tattatcaat aaaatcacta tttaaaaaaa aggtggcagg
     1261 aattcccatt tgtttaagaa ttggcgcaac aaccgaatgc atttcgctta gaccatcatc
     1321 aaatgaaagt acaaaataca ttttgccttt ttcataattt cctgcaacat aatcatctaa
     1381 atgcaatggc gtaaaatgtt gcgttaggaa cctcatttca tcttcaaaag caattgtgtc
     1441 ttttaccgga taaagatgtt tgatatgcgg aacagactga ttgctaacaa tatgataaaa
     1501 aggaaaaatg atgtgtacac ctgtattttt cgcaaggcgc tgcaaatccg catttttatt
     1561 gcgattgtta aacgtattaa aatataattg tttcaagctt tgcatgcacc tattgaataa
     1621 agcatgtaaa gctaaatcaa ttcacttaaa acgttctcct catttatcca tcttttacaa
     1681 ttaagcaaat aaatcgaaat ccagtttcaa ctggccggga cgcttaaacg atttgatatt
     1741 atattcaaaa gctttctttt ttggcatgta tttgtcgcgc atcaaataaa aaagttgatg
     1801 gatactatta gttaattcac catcaccgcg catcctgagt gaccagcgac tatcattaag
     1861 ttgtccctga tgagtagaac gaatattatt gagcactttt tctgctttca taggatatgt
     1921 tttccaaatc caatcggtaa aaattttatc gagtgcgccg tttaaacgaa ccattgtata
     1981 attggcagtt tgtgcaccgg catcggcagc agcattcatg atatcaggaa tttccgaatc
     2041 agtaagaccc ggtataattg gacctatcat tacaccgaca ggaattccgg cttctgccag
     2101 tttttcaatg gttttaagcc tgttgccaca gctggaggtg cgtggttcca actgacgacg
     2161 taaatcttcc ttcaaagttg taatagtaat atttacgaaa atcagatttt ctttcgccag
     2221 gtctttgagt atatcaatat cgcggagaat taattggttt ttggtgataa taccagccgg
     2281 gtttttaaac ttacggcata cctcgagtaa acttctggta atgcccaatt tccgttctac
     2341 gggctgataa caatcggtat ttccggaaaa agtaaccggt cccggcaccc atttttgctc
     2401 cataaatact ttttccagca attcggcagc gttatgttta acaataattt tagtttcaaa
     2461 atccaatccg gcgctaaacc cccaatagtt atgcgaattg cgcgcataac aatatataca
     2521 accatgttca cagccctgat acgggtttac agaatacatc atggcagcat cgggcatttc
     2581 caatttattg agtatttttt tgggctgttc aataaaatac tgcgtttcac gcggctccgg
     2641 taaggcttca tcgaggcctt caaagtgctc ttgcgtaagg ttggcattat tgaatggatt
     2701 aaaaggatta attt
//
LOCUS       DAORKW010000505         2709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2212_length_2709_cov_5.168048, whole genome shotgun sequence.
ACCESSION   DAORKW010000505 DAORKW010000000
VERSION     DAORKW010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2212_length_2709_cov_5.168048"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="PLB46_14730"
     CDS             <1..1090
                     /locus_tag="PLB46_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77830.1"
                     /translation="STSQKLGDSFTEFKITKANTSPYYLIYKYELSAGRIDKLFTLVM
                     DAETKIQQKNDINLPDDGYSPILVKEMITDEGQPIFLFENDDFNCKKDKTVTQYTFVF
                     PKNGNTWVSANINGGDYCLDEMNYAFDVLNNKLTAICFLKDDNKDYMIGYKYLTIDIY
                     SATIGTNFNYFYPTETLDEIAGLTKEKSIKNLPVYQIGSIIPRVDGGALMVAEYYDKT
                     VENYEYTNYDPYYGYRTSSRQVEFYEYDDILLFSIQPNGSPNWNTVIRKKQLSREDRG
                     VNSSYAILKGKESLIFIFNEDVEQNSNVLQYETTADGVLDRKSLFNPNQQEVQIRPLS
                     AEQISANEIIIPSIYKKNLSFVKLQIQK"
     gene            1094..2089
                     /locus_tag="PLB46_14735"
     CDS             1094..2089
                     /locus_tag="PLB46_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77831.1"
                     /translation="MLRIFHQSNPLTLLLLIVFTTLINLKWIISAEPITDHHYSFISN
                     ILFNNWLHINEWPVIVITLLHIGFIMITGILISFLLQQFKIITKASLIPAVVFITLCS
                     LFPDFIYGAPELLCGILLVFILFKIFGVYNKTKSDMTYFDVGLLSGLMSLLYFPAIVF
                     CLFGILSLFRIRSTSFREFFIYCVGLVLSYFLAATLLFWFDLLPQFAESQFGFPESLK
                     KMDSAFGVITIVKLSIVGIVFITSLVFFSNKFSTNLIQVRKYLSAFVYLLIFSTACMA
                     LNIPLHDGALYFVFIAASVFVSYYSYHFKNRLAPELMFLGLVGTTLIFQYINFAA"
     gene            2102..>2709
                     /gene="purQ"
                     /locus_tag="PLB46_14740"
     CDS             2102..>2709
                     /gene="purQ"
                     /locus_tag="PLB46_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRF77832.1"
                     /translation="MKFGVVTFPGSNCDQDLIHVLRDVMGCEVIELFHKQHDLGGLTT
                     DDCVMLPGGFSYGDALRTGSIARFSPIMQAVIKHCNAGGYAWGICNGFQILCEAGLLP
                     GVLLENNNQQFICKNVFLRADNNASRINATVPIGHALKIPIAHGEGRYYADDATLQQL
                     IANNQVIFSYCDEHGNVTDAANPNGSMHNIAGVCNAGRNVFG"
BASE COUNT          812 a          485 c          505 g          907 t
ORIGIN      
        1 ctctacatca caaaaacttg gcgatagttt taccgaattt aaaataacca aagcgaatac
       61 gtcgccgtat tatttaatct acaaatacga gttgagcgcc gggcgaattg ataaattatt
      121 tacattggta atggatgctg aaactaaaat tcagcaaaaa aacgatatca atttacctga
      181 tgatggttat agtcctattt tggtgaaaga aatgattact gatgaagggc aaccgatatt
      241 tttatttgaa aacgatgatt ttaattgtaa aaaagataaa actgttactc aatacacttt
      301 cgtatttcct aaaaatggca acacctgggt ttcggccaac ataaatggtg gcgactattg
      361 tttagatgaa atgaattatg cttttgatgt actgaataac aaattgacgg caatttgttt
      421 tttgaaggat gataataaag attacatgat tggttataag tatttaacca ttgatattta
      481 tagcgcaacc ataggtacta attttaatta tttttacccg accgaaacac tggatgaaat
      541 tgcggggttg acaaaagaaa aatcgataaa aaatcttccg gtatatcaaa ttggcagcat
      601 tattccgcga gtagacggcg gtgcattaat ggttgccgaa tattatgaca aaaccgtaga
      661 aaattacgaa tacaccaatt atgaccctta ttacggctat cgcacatcat cacgccaggt
      721 agagttttat gagtatgatg atattttatt attttctatt cagccgaatg gttctcccaa
      781 ttggaatact gtcatcagaa aaaaacaact gagcagagaa gaccgaggtg taaattcttc
      841 ctatgccatt ttaaaaggta aagaaagtct catttttata ttcaatgaag atgttgagca
      901 aaactcaaat gttttacaat acgaaacaac agcggatggt gtgttggata gaaaaagttt
      961 gttcaaccct aatcagcagg aagtgcaaat cagaccacta tcggctgaac aaatttcggc
     1021 taatgaaata attattccca gcatttataa aaagaacctt tcatttgtaa aacttcaaat
     1081 tcaaaaataa ttcgtgctga ggatattcca ccaatcgaac ccactaaccc ttttgttgct
     1141 gattgttttt accacactca tcaatttaaa atggattatc agtgctgaac ctattaccga
     1201 tcatcactat tcatttatca gtaatatttt attcaacaac tggctgcaca ttaacgaatg
     1261 gcctgtaatt gtaattaccc ttttgcatat cgggtttatt atgatcaccg gcattttaat
     1321 cagtttttta ttacaacaat ttaaaatcat tactaaagct tcgttaattc ctgcagtggt
     1381 atttattact ttatgcagtt tgtttccgga ttttatttat ggcgcaccgg aattattatg
     1441 cggcatttta ttggtgttta tcttatttaa gatttttggc gtgtataata aaacgaagtc
     1501 cgacatgacc tattttgatg ttggattgct aagtggtttg atgtcgctac tctattttcc
     1561 ggccattgtg ttttgtttat tcggtatatt aagcctattc agaatacgtt caacctcatt
     1621 ccgcgaattt tttatttatt gtgttggctt ggtattgagt tattttctgg cagccacctt
     1681 attattttgg ttcgatttgc taccacaatt tgcggagtcg caattcggtt tccctgaaag
     1741 tttgaaaaag atggattccg catttggtgt aattaccatt gtaaaactgt ctatagttgg
     1801 aatcgtattt atcacctcac tggtgttttt cagcaataaa ttttctacca atttgattca
     1861 ggtgcgcaaa tacctgagtg cttttgtgta tttacttatt ttttcaacag cttgtatggc
     1921 tttaaacata ccccttcacg acggggcttt gtattttgta ttcattgccg catctgtgtt
     1981 tgtgagttat tacagctatc attttaaaaa caggctggct cccgaactca tgtttttggg
     2041 gttagttggc actactttga tattccaata tattaacttt gcagcctaat tcattcagta
     2101 tatgaaattt ggcgttgtaa ccttccccgg ctctaattgt gatcaggacc ttatacatgt
     2161 tttacgcgat gtaatgggtt gtgaggtaat tgaattattc cacaaacagc acgatttagg
     2221 cggtttaact accgacgact gtgtgatgtt accgggagga ttttcatacg gagatgcact
     2281 cagaaccggc tctattgccc gtttttcacc aatcatgcaa gcggtaatta aacactgtaa
     2341 tgcaggtggt tatgcctggg gtatctgcaa cggattccaa attttatgtg aagcaggttt
     2401 gttaccgggc gtgttactcg aaaacaataa ccaacagttt atttgcaaaa atgtgttcct
     2461 aagggccgat aataatgcca gtcgcatcaa cgctactgtg ccaattggcc atgcactcaa
     2521 aataccaatt gcacatggtg aaggccgtta ttacgcagat gacgccactt tacaacaact
     2581 tatagccaac aaccaggtga tttttagcta ttgcgatgag catggcaatg ttaccgatgc
     2641 tgccaatccc aatggctcta tgcacaatat tgcaggtgtt tgcaatgcag gccgcaatgt
     2701 atttggcat
//
LOCUS       DAORKW010000506         2709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2213_length_2709_cov_4.271289, whole genome shotgun sequence.
ACCESSION   DAORKW010000506 DAORKW010000000
VERSION     DAORKW010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2213_length_2709_cov_4.271289"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..1132
                     /locus_tag="PLB46_14745"
     CDS             218..1132
                     /locus_tag="PLB46_14745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF77833.1"
                     /translation="MLKVNSITKTFDQFKAVNGISLSLTPGMFYGLLGPNGAGKTTTI
                     HMISAIMPPDTGDIQVAGIDVYTQQQKVKMHMGVVPQEIALYDELSAFDNLLFWGSLY
                     GITGADAKKQANYLLEWVGLADRKNDSVRTYSGGMKRRVNIASALMHNPGLIVMDEPT
                     VGIDPQSRNKIYELLDEMHAGGKTILYTTHYMEEAEKMCDKIGVIDSGKIIAEGSLNE
                     LKTNYSLDDTVVITYSGKSDGNIAGFATQHNEELNEIVIQIKDAKGHLSDIVNKCAMA
                     QIAIHNIDIKSSGLEAIFLHLTGKQLRD"
     gene            1153..2367
                     /locus_tag="PLB46_14750"
     CDS             1153..2367
                     /locus_tag="PLB46_14750"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF77834.1"
                     /translation="MWKITRKDLKIFFTDKRAIILSLLLPIGLITLFALAFGGVGAED
                     EEEGTPIAVLYADEDQTTSSKNIITELDAIPGIVCIATDTQSAKNQIKSGDQIANIII
                     HKGYEDALNNGADIPIELQYDQSRAMEISVLQNLLVSKISSVKGAKDADKGVDRIMQN
                     MFSNLPIQTQDSIRNNLKSGLNKENEQEQLITMTSIVGEEESNWGLIQAVAGTAIMML
                     LFSVRSIGSGMLEEKENGVLKKLLQSPITPFEILFGKMLTAVIVAIFQLSVMFLFSWL
                     AFGLDIFKNLPALIIMILTTAIACSAFGVLLASIVRSKKQADSLSTIIILFMSAIGGS
                     MIPLYIMPAFMQDAAVISVNYWSIQGFYDIFWRETGVAGVTQNAIVLIAITAGVLAIS
                     AFFFKKNILRIS"
     gene            complement(2372..>2709)
                     /locus_tag="PLB46_14755"
     CDS             complement(2372..>2709)
                     /locus_tag="PLB46_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-carotene hydroxylase"
                     /protein_id="HRF77835.1"
                     /translation="HEPRHGKFELNDLYAIIFAAPAIVLMYIGSPEYNYVFWIGSGIT
                     AYGFCYFLFHDVIVHRRMKHQYKPHSKYMLRIIRAHKVHHKKMSKEEGQAFGFLFAHP
                     KFDVKKTDE"
BASE COUNT          881 a          395 c          548 g          885 t
ORIGIN      
        1 attttccaat ataacaaatg gtaatcgtta tctattgcat tctgcagtaa ttgatgaaat
       61 tcctccttat tgccgctaaa tatcaaatca ccggttttaa agccaaaaaa atctgaatcc
      121 cattttttta aaactatttc cggcatttat gcaatttttt tgctttgagt aatctgctta
      181 ctaaattaat taatacattt accatttaat aaccgctatg ctgaaggtaa attctatcac
      241 aaagaccttt gatcaattta aagctgtaaa cggaatcagt ttatcgctta caccgggtat
      301 gttttatggt ttgttaggac caaatggcgc cggtaaaact acaacaattc atatgatcag
      361 cgcaattatg cctcctgata caggtgatat tcaggttgct gggattgatg tatataccca
      421 acagcaaaag gttaaaatgc acatgggagt tgtacctcag gaaattgcct tatatgatga
      481 actgagtgct ttcgataatt tacttttttg gggaagtttg tatggtataa ccggcgctga
      541 tgcaaaaaaa caagctaact atttattgga atgggttggg ttggccgacc ggaaaaatga
      601 ttctgtgaga acatattcag gcgggatgaa gcgaagagtg aatattgcat ctgcattgat
      661 gcataatccg ggtttaattg ttatggatga gccgacagtt ggcattgacc ctcaaagcag
      721 gaataaaatt tatgagttat tggatgaaat gcatgccggt ggtaaaacaa ttttatatac
      781 tacacattat atggaggaag cagaaaaaat gtgtgataaa attggcgtta tagatagtgg
      841 aaaaattatt gctgagggta gtttaaatga acttaaaaca aattattcgt tagatgatac
      901 ggttgttata acttattcgg gcaaaagcga tggtaatatt gccggttttg ctacgcaaca
      961 taatgaagaa ttaaacgaga tagttattca gattaaagat gcaaaagggc atctttcaga
     1021 tattgttaat aaatgtgcga tggcgcaaat cgcaattcat aatatcgata taaaaagcag
     1081 tgggttagaa gcgatatttt tacatttaac aggaaaacag ttgcgtgatt agttatttaa
     1141 atacaagcaa ttatgtggaa aataacacga aaggatttaa aaatattttt tactgataaa
     1201 agagctatca ttttatcatt attgttacca atcggattga taacactttt tgcattggca
     1261 tttggtggag taggtgcaga ggatgaggag gaaggcacgc cgatagctgt gttatatgct
     1321 gatgaggatc aaactacatc ttcaaaaaat attattactg agcttgatgc catacccgga
     1381 attgtttgta ttgctactga cactcaaagt gcaaaaaatc aaattaaaag tggcgaccaa
     1441 attgcgaata ttattattca taaaggttac gaagatgcgc taaataacgg tgccgatata
     1501 cctattgaat tacagtatga ccaaagccgt gcgatggaaa taagtgtgct gcagaattta
     1561 ttggtaagta aaatttcttc cgttaaaggt gctaaggatg cagacaaagg tgtggacagg
     1621 attatgcaaa atatgttttc aaatttgccc attcaaacac aggactcgat tcgcaacaat
     1681 ttaaaatcag gattaaacaa agaaaatgaa caggaacaat taatcacaat gaccagcata
     1741 gttggagagg aagaaagtaa ttggggttta attcaggctg ttgcaggaac tgcaatcatg
     1801 atgttgttat ttagtgttcg ttccattggt tccggtatgt tagaagagaa ggaaaatggt
     1861 gtgttaaaaa aattattaca atcgcccatt acacctttcg aaattttgtt tgggaaaatg
     1921 ctgactgccg ttattgttgc tattttccaa ctgtcggtca tgtttctatt ttcatggtta
     1981 gcatttggat tggatatttt taaaaatttg cctgcattaa ttataatgat tttaactaca
     2041 gcaattgcct gttctgcatt tggtgtattg ctggcgagta ttgtgcgttc gaaaaagcag
     2101 gctgattcgc tgagtacaat aattatttta tttatgagtg caattggcgg gagtatgatt
     2161 cccttgtata ttatgcctgc ttttatgcaa gatgcggctg taatttcagt taattactgg
     2221 agtattcaag ggttttatga tattttttgg cgtgaaaccg gtgtagcagg tgtaactcaa
     2281 aatgctatcg ttttaatagc aattactgca ggtgttttag caattagtgc attctttttt
     2341 aagaaaaata ttttgcgaat ttcttaatta attattcgtc ggtttttttt acatcaaatt
     2401 taggatgtgc aaataagaac ccaaaagcct ggccttcttc tttactcatt tttttatgat
     2461 gcactttatg tgcgcgtatt atacgcaaca tatatttgct atgtggttta tattgatgtt
     2521 tcatacggcg atgaacaatt acatcgtgaa acaaaaaata acaaaatcca tatgcggtaa
     2581 ttcctgagcc aatccaaaat acataattgt attcaggtga accaatatac attaatacaa
     2641 ttgcaggagc tgcaaatatg atagcatata agtcgttgag ttcaaattta ccatgacgtg
     2701 gttcgtggt
//
LOCUS       DAORKW010000507         2700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2225_length_2700_cov_5.294518, whole genome shotgun sequence.
ACCESSION   DAORKW010000507 DAORKW010000000
VERSION     DAORKW010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2225_length_2700_cov_5.294518"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLB46_14760"
     CDS             <1..872
                     /locus_tag="PLB46_14760"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77836.1"
                     /translation="AVVPIGVEDCFDVAAYGVVVQVDPVGSPNNVKVNLITPGYYPFP
                     ATYFGGTDLEFKAIADPTFTFDHWEFNNHSPLPSTTDDSVYVSLTSADTVTAWFAGTE
                     LPNYDFTLDVYPPGAGTASVEAFYPGTYPYSTVYVSGNMMDLVATPAPGFAFDYWELE
                     NHIANPDPFTADIFFTISDLENATAHFRTTNAIDDVTANIGLDVSPSVTNGPVTINYQ
                     LSANQEDIRIELFSVTGQLIAIINQQQNVSGTPGEHTVTINLQDYRLADGMYFIKLTA
                     GNESQNERVIMNH"
     gene            984..2258
                     /gene="glyA"
                     /locus_tag="PLB46_14765"
     CDS             984..2258
                     /gene="glyA"
                     /locus_tag="PLB46_14765"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRF77837.1"
                     /translation="MQRDTAVFDIINKELHRQTEGIELIASENFVSPQVMEAMGSCMT
                     NKYAEGYPGKRYYGGCEFVDETEQLAIDRVKKLFGAEFANVQPHSGAQANAAVMLACL
                     NPGDTLLGFNLSHGGHLTHGSPVNFSGKLYRPVFYSVEEATGRIDMDKVEAIAKAEKP
                     KLIIAGASAYARDWDYARFRAIADSVGAILMADISHPAGLIASKLLNDPIPHCHIVTT
                     TTHKTLRGPRGGLIMIGKDFPNPWGHTTPKGEVKMMSAIIDSAVFPGTQGGPLEHVIA
                     SKAIAFGEDLSESYVGYCKQIIANAQAMAKAFIGKGYKIISDGTDNHLMLIDLRNKNI
                     SGKKAENLLGAAHITVNKNMVPFDDKSPFVTSGIRIGTPAVTTRGMKENDMVKIVEWI
                     DRLIMSPDDETMIAAVRSEVTAFAKSFPLFAY"
     gene            2274..2648
                     /locus_tag="PLB46_14770"
     CDS             2274..2648
                     /locus_tag="PLB46_14770"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77838.1"
                     /translation="MKKIFVVVIAFIMGNQMFAQTGCIQGNCEKGTGTYRWDNGDTYT
                     GKFKKNMKHGKGEFTFASGDKYVGSFAKDNYEGEGVYTFANGSSYSGTFKAGDYNGKG
                     VYKYDNGEVYTGMFANNLKNGK"
BASE COUNT          855 a          466 c          562 g          817 t
ORIGIN      
        1 gggctgttgt accaattggt gttgaagatt gttttgatgt tgcagcatac ggcgttgtag
       61 ttcaggttga tcctgttgga agccctaaca atgtgaaagt gaatttaatt actccgggtt
      121 attatccgtt tcctgcaact tattttggtg gaaccgattt agaatttaaa gcaatcgccg
      181 acccaacatt tacattcgac cattgggaat ttaataatca ctcaccgctg ccaagtacta
      241 cagatgatag cgtttatgta tcattaacca gtgccgatac cgttactgca tggtttgcag
      301 gaacagaatt acctaattat gattttacat tagacgtata tcctccgggc gcaggaactg
      361 cttctgttga agcgttttat cctggaactt atccttatag caccgtatat gtaagcggta
      421 atatgatgga tttagttgct acaccggcac ctggatttgc tttcgattat tgggaattag
      481 aaaaccatat tgctaaccct gatccattta ctgctgatat tttctttacg atttccgatt
      541 tagaaaatgc gacagcacat ttcagaacaa ctaatgcaat tgatgatgta acagctaaca
      601 tcggtcttga tgtttcacca tcagttacca atggtcctgt tactattaat tatcagttaa
      661 gtgcaaatca ggaagatatc cgtatcgaat tatttagtgt aactggtcag ttgattgcta
      721 taattaatca acaacaaaat gtttccggca cacctggtga acatacagtt actattaatc
      781 ttcaggatta tcgtttagct gatggcatgt attttattaa attaactgca ggcaacgaat
      841 cacaaaacga aagagtgata atgaatcatt aatttaaatt aatttaatta tagtaaggct
      901 gccggataaa ggcagccttt ttttgtttta tcatagaatt cttactcacc attaattaca
      961 tttgccaaat caaataacca actatgcaaa gagataccgc agtattcgac attattaata
     1021 aagaattaca tcgtcaaacc gaaggcattg aacttattgc cagtgaaaat tttgtcagcc
     1081 cacaggtgat ggaagccatg ggtagttgta tgaccaataa gtatgcagag ggttatcccg
     1141 gcaaacgata ttatggcggt tgcgaatttg ttgatgaaac agaacaactg gcaatcgaca
     1201 gagtgaaaaa attgttcgga gccgagtttg ccaatgtgca accacatagc ggtgctcagg
     1261 caaatgcagc agttatgctg gcttgtctaa atcccggtga tactttactt gggtttaacc
     1321 tttctcatgg tggtcacctc acacatggca gtcctgtgaa tttttccggt aaattatacc
     1381 gaccggtatt ttattcggta gaagaagcaa caggtcgtat cgatatggat aaggtggaag
     1441 ctattgccaa agcagaaaaa cctaaactta ttattgccgg cgcaagtgct tatgctcgcg
     1501 actgggatta tgcccgtttt agagcaattg cagatagcgt tggggctatt ttaatggcag
     1561 atatttcgca ccctgccgga ttaattgctt ccaaattatt aaatgaccct atccctcatt
     1621 gtcatatagt tactactact acacataaaa cattacgcgg acctcgtggt ggcttaatta
     1681 tgattggaaa agattttcca aacccttggg gacatacaac tccaaaaggt gaagtgaaaa
     1741 tgatgagtgc tattattgat agtgctgtat ttccgggaac tcaaggcgga ccgctggaac
     1801 atgttatcgc atcaaaagca attgccttcg gcgaagattt aagtgaatcg tatgttggtt
     1861 attgcaaaca aataattgcg aatgctcaag caatggcaaa agcatttatt ggaaaaggat
     1921 ataaaattat ttctgacgga accgacaatc atttaatgtt gattgattta cgcaacaaaa
     1981 atatttccgg aaaaaaagca gaaaatcttt taggtgctgc tcatattacg gtaaataaaa
     2041 atatggtgcc ttttgatgat aaatcgccat ttgttactag tggaatcaga attggtacac
     2101 ctgctgttac tacacgtggt atgaaagaaa atgatatggt gaaaatagtt gagtggattg
     2161 acagattgat tatgtcgccg gacgacgaaa ctatgattgc tgcagtgcgc tcagaagtta
     2221 ctgcatttgc gaaatcattt ccattatttg cttactaaaa aataattcaa aatatgaaaa
     2281 aaatatttgt tgtagtaatt gccttcataa tgggcaatca gatgtttgca caaacaggat
     2341 gcattcaggg aaattgtgaa aaaggaaccg gaacttaccg ttgggataat ggtgatacgt
     2401 ataccggaaa attcaaaaag aatatgaaac acggtaaagg tgaattcact tttgccagtg
     2461 gtgacaaata tgtaggctcc tttgcgaaag ataattatga aggcgaaggt gtttatacgt
     2521 ttgctaatgg cagttcgtat tccggcacat ttaaagcagg tgattacaat ggaaaaggtg
     2581 tatataaata cgataacggt gaagtgtata ccggcatgtt tgcgaacaat ttaaaaaatg
     2641 gtaaatgagt ttacaaattt acggatggta gttcttattc cggcgaattt aaaaacgacc
//
LOCUS       DAORKW010000508         2696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2232_length_2696_cov_6.241575, whole genome shotgun sequence.
ACCESSION   DAORKW010000508 DAORKW010000000
VERSION     DAORKW010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2232_length_2696_cov_6.241575"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLB46_14775"
     CDS             <1..314
                     /locus_tag="PLB46_14775"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77839.1"
                     /translation="AAAPAALVRKIKKISWDAYVSNGGKGYTRVDLRMDKFSGKISVL
                     EVNAQCGLSEDENYTSIGAILRYSNKSFTQMVIEIINDATYRWMKKNRKRKVPALVKA
                     Y"
     gene            332..1813
                     /locus_tag="PLB46_14780"
     CDS             332..1813
                     /locus_tag="PLB46_14780"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HRF77840.1"
                     /translation="MHISADNIVIPENGKGLKICVLQPDYSTSDVDYQYYDPARDLSS
                     LLPEAEFKHVFLNKLTTYKQLRELKKEGFDIFVNLCEGYLDWSIPSVDVIYYMELLGL
                     PFTGPTSILYDPTKDLMKYVAFTAGVKTPPFALISGNENLDDATAHLQYPLFVKPAKA
                     GDSLGVDDSSLVKNKTELITKVNAIIEEYGPLLVEEYIDGREFTVMIAANPYDRSKPY
                     LFKPIEYIFPQNFSFKTYALKTSELHPNANIPVDDSVIVEKLHTATRQIFEGFNGVGY
                     ARLDFRMNAKGEIFFLEINFTCSVFYKDGYEGSADYVLREDGLGQKGFLQLIILEGIA
                     RYRRTHKPFRLQGNSKAGYGIFATRVIYKGEVIFPGEESAQRIVTKQYVEKNWSEEAK
                     EVFRRYAYPISNEVFLLWDNDPNGWAPQNHSCVPNTEYDGLNVNAISDIQIGDELTLD
                     YTTFLDENMEPFECRCGHPTCRGLIKGTPGNSITNIAIRKRNI"
     gene            2125..>2696
                     /locus_tag="PLB46_14785"
     CDS             2125..>2696
                     /locus_tag="PLB46_14785"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77841.1"
                     /translation="MKSIFIPRLLQFIVASFFLILSVNSKAQISGNDTVCIGDIVTYT
                     TSLSGTITWSVSPSGPVITASGSTAVITWSSAANTYIITANNGTSTETFNVVSFALPN
                     PVISYQTNACSAVPHEPGGGPAGDAKCDTFCIDEIVTYSVINVPGNTYSWTIFGGTII
                     SGFGTNSVEVAWGTGPNCGYLCLTESNPAG"
BASE COUNT          857 a          451 c          508 g          880 t
ORIGIN      
        1 atgctgctgc acccgctgcg ttagtcagaa aaataaaaaa aattagttgg gatgcatatg
       61 tttccaatgg tggcaaaggt tatacccgtg tagatttacg tatggataaa ttttcaggta
      121 aaatttcggt tttagaagta aatgcacaat gtggtttaag tgaagatgaa aattatactt
      181 ctataggtgc aattttacga tatagcaata aatcgttcac gcaaatggtt atagaaataa
      241 ttaatgatgc tacctatcgc tggatgaaaa aaaatcggaa gcgaaaagtt ccggcattag
      301 ttaaagcata ttaatttata aaatctacac attgcatata agtgctgaca atattgtaat
      361 accggaaaat ggaaaaggac taaaaatttg tgtcctgcaa cccgattact ccacatcaga
      421 tgtagattac caatattatg accctgctcg cgatttgtcc tcattacttc ctgaagctga
      481 gtttaaacat gtatttttaa ataagctgac tacttataag caattaagag aattaaaaaa
      541 agaaggtttt gatattttcg tcaacttatg cgaaggttac ctcgactggt ctattccatc
      601 ggtagatgtt atttattaca tggaactttt aggtttacca tttacgggtc ctacttccat
      661 tttatatgac cctaccaaag atttgatgaa atatgtggcg ttcactgcag gggttaaaac
      721 accaccattt gcactgattt ccggtaatga aaatctggac gatgctactg cacatttgca
      781 atatccatta tttgtaaaac cagccaaagc cggcgatagt ttgggtgttg atgacagctc
      841 gttggtaaaa aataaaacgg agttaattac aaaagtaaat gccattattg aggaatatgg
      901 tcctttatta gtagaagaat atattgatgg gagagaattt accgtaatga ttgctgcaaa
      961 tccttacgac cgttctaaac cgtatttatt taaaccaatt gaatatattt ttccgcaaaa
     1021 ttttagtttt aaaacatatg cgttaaaaac ttcagagtta catccgaatg ccaatattcc
     1081 tgttgacgat tcagttattg tagaaaaatt acatacagca acccgacaaa tatttgaagg
     1141 atttaatggt gttggatatg cgcgtttaga ttttaggatg aacgccaaag gggaaatatt
     1201 ttttctggag ataaacttca cctgttctgt tttttataaa gatggttatg aaggatctgc
     1261 cgattatgta ttacgtgagg acggactcgg gcaaaaagga tttttacaac tgataattct
     1321 cgaaggtata gcacgttatc gacgtaccca taagcctttc agattgcagg ggaattcaaa
     1381 agcgggttat ggcatttttg ctaccagagt tatttataag ggagaggtaa ttttcccggg
     1441 tgaggaatca gctcagcgta tagttaccaa acaatacgtt gaaaaaaact ggtcggaaga
     1501 agcaaaggaa gtatttagaa gatacgccta cccaatcagc aatgaagtat ttttattgtg
     1561 ggataatgac cctaacggat gggctccgca aaatcacagt tgtgtgccca ataccgaata
     1621 cgatggttta aatgtaaatg ccataagtga tattcaaatt ggtgatgaat taacgttaga
     1681 ctatactact tttttagatg aaaatatgga gccttttgaa tgccgttgcg gacatccaac
     1741 ttgtcgcggt ttaattaaag gtacacccgg taattcgatt actaatattg caatacgcaa
     1801 acgcaatata taatacatca agataatttt taccgtttat ttttttaaac aaccgtttac
     1861 tcattattat cacagccctt ttgttaaatg ggtttgtcaa taattttttt taaaaattaa
     1921 ttttacatta gtttaatttt aattcccacc gaattaattt tctgataatt taactgattt
     1981 gctacaagtt gaggttgttg gttatttcca cttattaagt caacccttgc acttattaca
     2041 taattgattg caattacatg taagatactt gaatttaagg aaataatact ttatattaca
     2101 tgcaattacc ctaaattcta cattatgaaa tctattttta tcccacgttt gctccaattt
     2161 attgttgctt cgtttttttt aattttatct gtaaactcca aagctcagat ttcgggcaat
     2221 gatacggttt gtattggtga tatagttacc tatactacct cattatccgg aaccataacc
     2281 tggtcggttt ctccatcagg accggttatt acagcttccg gaagtactgc tgttataaca
     2341 tggtcttcgg cagcaaacac gtatatcatt acggctaata atggtacatc aacggaaacg
     2401 tttaatgttg tttcatttgc gctgcctaat cctgttatca gctatcaaac caatgcctgc
     2461 tcagcagttc cgcatgaacc cggaggaggc ccggccggag atgcaaaatg tgatacattt
     2521 tgtattgatg aaatagttac ttacagtgtg ataaatgttc cgggtaatac ttactcatgg
     2581 accatttttg gtggaacaat tatcagcggc tttggaacaa attctgtaga ggtagcatgg
     2641 ggaaccggac ctaattgcgg atatttatgt ttaactgaat ccaatcctgc aggttg
//
LOCUS       DAORKW010000509         2695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2234_length_2695_cov_4.640152, whole genome shotgun sequence.
ACCESSION   DAORKW010000509 DAORKW010000000
VERSION     DAORKW010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2234_length_2695_cov_4.640152"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PLB46_14790"
     CDS             complement(<1..1023)
                     /locus_tag="PLB46_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HRF77842.1"
                     /translation="MDSKTAKIAAFNANGVGVANNQLFGLPFTYEESELVLFSAPWEV
                     TVSYRAGTAKGPDAMLKASTQIDLYDNDNPNGWHNGIFTKKSSATIKKKNTEVRKKAA
                     AHIKALEKGKSVNADELITEINAECEKMNAYVYNEASAILADGKIPALIGGDHSTPLG
                     LIKALGEKHGGFGILQIDAHADLRDAYEGFTYSHASIMFNALQNEQVKKLVQVGVRDI
                     CDDEINLINASNRRIVTYFNHEIKDAVFNGINWTAICRQIVEELPKKVYISFDIDGLD
                     QRYCPNTGTPVPGGLAYEQAVYLLNEIITSGREIIGFDLVEVAPGDDEWDAIVGARML
                     YKMCNLA"
     gene            complement(1116..1958)
                     /locus_tag="PLB46_14795"
     CDS             complement(1116..1958)
                     /locus_tag="PLB46_14795"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF77843.1"
                     /translation="MTISKIQLFISVLLLSGGLQAQVFSGKVLDATTAETLPFVSIGI
                     PGKSVGTVAQLDGTFNITISDVYNNDSIRFSIIGYEAKKMLVSEFKALAAAQKAVIKL
                     QPQDEILGEVMIRPRDMKSKILGNEFDSPSIIAGFTSDDLGSEIGTVMKIKDGKDYYL
                     KNCGFNFGRVDYDSIIFRINIYNIKNGLPGDLLHQLPIYITVYRDQRSAEVDLRPYNL
                     HIDQDFILSLEWVQDLPEKTKSLMFCAGFFGNKVVYRQVAEDVWRDFPIGIGMWCDAE
                     YEKH"
     gene            complement(2078..>2695)
                     /locus_tag="PLB46_14800"
     CDS             complement(2078..>2695)
                     /locus_tag="PLB46_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRF77844.1"
                     /translation="VDAVFNHRKSSGSILNFPGATNINATAEILEQPCDILIPAALEN
                     VIHEGNASKIKAKIIGEAANGPVTAEADDILTDMGVMIVPDMYLNAGGVTVSYFEWLK
                     NLSHVRFGRLQKRFEQGSNSRIVEAIEELTGKKLNAVEVDKIIKGPDEIDLVRSGLEE
                     TMINSYHAIRNEWKSNVACKNLRTAAFVVAINKIASDYLALGIFP"
BASE COUNT          835 a          552 c          440 g          868 t
ORIGIN      
        1 tgcaaggttg cacattttat acaacattct tgcgccaaca attgcatccc attcgtcatc
       61 accaggagct acttcaacta aatcaaaacc aataatttcg cgtcctgaag taataatttc
      121 atttaataaa taaacagctt gctcataggc taatccaccc ggcacaggtg ttccagtatt
      181 tggacaataa cgttgatcta acccatcaat atcgaaactg atatatactt ttttaggtaa
      241 ttcttctaca atctgacgac aaatggctgt ccaattaata ccattaaaaa cggcatcttt
      301 tatttcgtga ttaaaataag taacaatacg acggttagag gcgttaatta gattaatttc
      361 atcatcacaa atatctctta caccaacctg cactaatttt ttaacctgct cattttgtaa
      421 tgcgttaaac ataatactgg catgcgaata agtaaaccct tcataagcat cacgtaaatc
      481 ggcatgtgca tcaatttgca atatgccaaa tccaccatgt ttttcgccta gtgcttttat
      541 caatccaagc ggagtgctat ggtcaccacc aattaaagca ggaatttttc catctgctaa
      601 aattgcagat gcttcattat aaacataagc attcattttt tcgcattcgg catttatttc
      661 tgttatcaat tcatctgcat ttactgattt gcctttttct aatgctttaa tatgtgcagc
      721 agcttttttt ctaacttctg tatttttctt tttaatagtt gcagaacttt ttttcgtgaa
      781 aataccatta tgccaaccat tcggattatc gttatcgtac aaatcgattt gcgttgatgc
      841 ttttaacatg gcatcaggtc ctttagcagt tcctgcacga taggaaacag taacttccca
      901 aggggcagaa aataatacga gttcactctc ttcgtaggtg aaaggcagtc caaaaagttg
      961 attattggca acccctactc cattggcatt aaatgcggct atttttgcag ttttcgaatc
     1021 catgcaacaa agataaggat tgagcaacca aactgtgtag gatatgcctg ttatccgcta
     1081 ttgatatggc ttacctcaag ccaaagctga gcgctttaat gcttttcata ttcagcatca
     1141 caccacatgc ctattccaat tgggaaatcg cgccatacgt cttctgcaac ctgacgatat
     1201 actactttat taccaaaaaa gccggcgcaa aacatgagcg atttagtttt ttccggtaaa
     1261 tcctgcaccc attccagtga taaaataaaa tcctggtcga tgtggaggtt gtagggacgt
     1321 aaatcaactt cggcactgcg ttgatcgcga taaacggtaa tataaattgg cagttggtgc
     1381 aataaatcac cgggcaaacc attttttatg ttgtagatat ttatacgaaa aataatgctg
     1441 tcataatcca ctcttccgaa gttaaatccg cagtttttta agtaataatc tttgccatct
     1501 ttaatcttca taacggttcc tatctctgaa cccaagtcat cggaagtaaa tcctgcgatg
     1561 atagacggag aatcaaattc gttacctaat attttagatt tcatatcgcg tgggcgaatc
     1621 atcacttcgc ctaaaatttc atcttggggt tgcaatttaa taacagcctt ttgagctgca
     1681 gctaacgctt taaattcact caccagcatt tttttcgcct cataacctat aatagaaaaa
     1741 cgaatggaat cattgttgta aacatcgctg atagtaatgt taaaagtgcc gtccaattgc
     1801 gcaactgttc caacagattt tccgggaata ccgatgctca caaatggcaa tgtttctgct
     1861 gttgtcgcat ccaacacttt gcctgaaaat acctgtgcct gtaggcctcc tgagagtaat
     1921 aaaacgctta taaaaagttg aatttttgag attgtcatcc gtattgtttt ggagtatgac
     1981 gatggtaagg ttacaaatgt tacagcgtga taattatatg acaaaaaaaa gtcccgatgt
     2041 aaaaacaccg ggacaaaata tactttgttg caattaatta agggaaaata cccaatgcta
     2101 aataatcgct tgcaatttta ttgatagcta caacaaatgc tgcggtgcgt aaatttttgc
     2161 aggcaacatt tgatttccat tcgttgcgga tagcatgata agaattaatc attgtttctt
     2221 ccaaacctga acgaactaaa tcaatttcat ccgggccttt aatgatttta tccacttcaa
     2281 cagcgtttaa ttttttgccg gttaattctt caatagcttc aacaattctg ctattgcttc
     2341 cttgttcgaa acgtttttgt aaacggccaa aacgaacgtg gcttaagttt tttaaccatt
     2401 cgaaataaga aacggtaaca ccacctgcat ttaaatacat atcaggaaca atcattacac
     2461 ccatatcagt taaaatatca tcagcttcag cagtaacagg accgttagcc gcttcaccaa
     2521 taattttagc ttttatttta gatgcattgc cttcatgaat tacattttct aatgcggcag
     2581 gtattaaaat atcacaaggt tgttccaata tctctgcagt tgcattaata ttagtagcac
     2641 cagggaaatt taaaatactt cctgaagatt ttctgtgatt aaatactgca tcaac
//
LOCUS       DAORKW010000510         2695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2235_length_2695_cov_4.512121, whole genome shotgun sequence.
ACCESSION   DAORKW010000510 DAORKW010000000
VERSION     DAORKW010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_2695_cov_4.512121"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLB46_14805"
     CDS             <1..672
                     /locus_tag="PLB46_14805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77845.1"
                     /translation="TNSIPTYEYDGSYFKYQYYAETPTVTIVDTVGNGSAIQVNFPMF
                     NNEDDLELYRIFVAKADDVVSPEIVSTISAIKRLDITPTGANLDINLHDLLHDIDGNS
                     IQYDQPYKVYVALKGDFYPDYYSLAEPSAEFILEKNNVDPAVNQMPNATPEIWVNNNI
                     AHMQGFTEEEHAIVLYNALGEILLNTNFSGADFSLLLPELPTGIYVISVMGAGSVYQQ
                     SVYVY"
     gene            751..2676
                     /locus_tag="PLB46_14810"
     CDS             751..2676
                     /locus_tag="PLB46_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77846.1"
                     /translation="MKTLQLNTYASLAAVFLASQKQADAQLIFTEVDPDKIVNSYTNY
                     GLDFNDDGIDEIVFEPDYFYTWTSFGGASWGYSSQNMDLLNYANVAFSESRPMFLPLG
                     DVVDDTKPFTSGSLNLFYGKFNISPETLEMFSGESWINTEGFIGVKFNIDANVHYGWV
                     RVTLNHVEDGRLPSLTIKDYAYNATPGEGAETMLFSASTAKNLILTDIGETNTANDLQ
                     LQFDKAVEESTVSAYRVILDKSYPYPTLAEANLLPPERYTEILPTGSDITIQFNETTL
                     DVDGNPLIPATNYRAYVLSVADGVIVTVNDISIASNAAQYSFVEASPAAYPNLAVNYI
                     DDDMSAFYVTYNFYNTDVDTIKVFITDYIYYELDELLTFSPDYYVISNPVIGTNTVTF
                     TADNLVYLSETPVLFNKYYCYLVSVPDGTESSFYSYTFTTDYFLYPYTDIAPEIINSN
                     ISGNITDIHVNYPMWENEEKINEYLIVVVPEGQLFTNMMVDTLGFTEAYDFPPNGEDI
                     SKDMLLSARDVNGDVIVPGIYYHIYVVLKGNVSPVYYTCSQPSAAFMLTAPLSASAIE
                     NTIAMECIGTTLHVMVSNLNTANMQIVNAAGVIVYQNKLVNTESAIDLIFLPAGMYIA
                     TVTTENKKTNFVFPLTK"
BASE COUNT          877 a          480 c          473 g          865 t
ORIGIN      
        1 accaattcta tcccgacata cgagtacgat ggttcatatt tcaaatacca atactatgcc
       61 gaaacaccca cagttactat agttgataca gttggcaacg gctcagccat tcaagtaaat
      121 ttccccatgt ttaataatga agatgatttg gaactttatc gaatttttgt tgcaaaagct
      181 gatgatgtcg tatcgcctga aattgtttct acaatatcag caataaagcg actagatata
      241 acaccaacag gcgcaaatct ggatatcaat ttacatgatc ttcttcacga tattgatggc
      301 aacagtattc agtatgacca accttataaa gtttatgttg ctttaaaggg agatttttat
      361 cctgattatt attcactcgc agaaccatca gcagaattta ttttagaaaa aaacaatgta
      421 gatcctgctg tcaatcaaat gccaaatgct acacctgaaa tttgggttaa caataatatt
      481 gcacatatgc aaggatttac ggaggaagaa cacgcaatag tgttgtacaa tgcacttgga
      541 gaaatattat tgaatacgaa tttttcaggg gctgattttt cattattgct tccggaattg
      601 cccaccggaa tttatgtgat aagtgtaatg ggtgcaggat cagtatatca acaatcggta
      661 tatgtgtatt agtcaataca atttatccgg tatttcaatt atacgatgct aatcaaataa
      721 taaataatag ctttaacaca acctccaaac atgaaaaccc tgcaattaaa cacctacgct
      781 tcactagccg cagtattcct tgcttcacaa aagcaggcag atgcacaatt gatatttaca
      841 gaagttgatc ccgacaaaat agtaaatagt tataccaatt atggtctcga ttttaatgat
      901 gacggaatcg atgaaatagt ttttgaacct gattactttt atacctggac atcttttggt
      961 ggagcatcat gggggtatag ttctcaaaat atggatttat tgaattatgc taatgtagct
     1021 ttttcagaaa gcagaccaat gtttttacca ttaggagatg tggttgatga tactaaaccc
     1081 tttacttcag gcagcctcaa tttgttttat gggaaattta atataagccc cgaaacactc
     1141 gaaatgtttt caggagaaag ctggataaat actgaaggat ttatcggtgt aaaatttaat
     1201 atagatgcta atgtccatta tggttgggtg cgtgtcaccc taaatcatgt tgaagatgga
     1261 cgtttgccca gtttaacaat taaagattat gcctataatg ccacccccgg cgaaggcgct
     1321 gaaacaatgt tgttttcggc atctactgct aaaaatttaa ttcttaccga tatcggagaa
     1381 acaaataccg caaacgattt acaattacaa tttgataaag cagtagaaga atcaaccgtc
     1441 agcgcttatc gcgttatttt agataaaagt tatccctacc caactttagc tgaagctaat
     1501 ctgttgccgc ctgagcgata cacggaaata ttacccaccg gttccgatat caccatccaa
     1561 ttcaacgaaa ctactttaga tgttgatggc aatccattaa taccggcaac caattatagg
     1621 gcctatgtgc tatcagtagc agatggagtg atagttacag taaatgatat ttccattgct
     1681 tcaaatgctg cgcaatattc atttgttgag gcatcacctg cggcatatcc caatttggca
     1741 gttaattata tcgacgatga tatgtcggct ttttatgtca cgtataattt ttataataca
     1801 gatgtagata cgattaaagt atttattaca gactatattt attacgagtt agatgagctg
     1861 ttgacatttt cacctgatta ttatgtaata agtaaccctg tgattggcac taatacagta
     1921 acatttacgg ccgataactt agtatattta agtgaaacac ctgtgctttt taataaatac
     1981 tattgttatt tagtttctgt gcccgatggt actgaaagta gtttttattc ctatacattt
     2041 accaccgatt attttttata tccctatact gatattgcac ccgaaataat taattccaat
     2101 atttccggca acattaccga tattcatgtg aattatccta tgtgggaaaa tgaagaaaaa
     2161 ataaatgaat atctaatagt ggttgttcct gaaggtcaat tgtttactaa tatgatggtt
     2221 gacacattag gatttacgga ggcttatgat tttccaccga atggtgagga tatttccaaa
     2281 gacatgctgc tatctgcacg tgatgttaac ggcgatgtga ttgtgcctgg aatttattat
     2341 catatatatg ttgttttaaa aggaaatgta tctcctgtat attatacctg ctcccaacct
     2401 tccgctgcat ttatgttaac agcgccgttg tcagcgagtg ccattgaaaa tactattgca
     2461 atggaatgta taggaacaac cttacatgta atggtatcca atttaaatac agccaacatg
     2521 caaattgtca atgctgcagg tgtaatcgtg tatcaaaaca aattagtcaa cactgaatct
     2581 gcaatcgacc tcattttttt gccagcaggt atgtacatcg caacggttac cactgaaaat
     2641 aaaaaaacga attttgtatt tccattgaca aaataaaata ttgatataat taatt
//
LOCUS       DAORKW010000511         2692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2239_length_2692_cov_5.632537, whole genome shotgun sequence.
ACCESSION   DAORKW010000511 DAORKW010000000
VERSION     DAORKW010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2239_length_2692_cov_5.632537"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2692
                     /locus_tag="PLB46_14815"
     CDS             <1..>2692
                     /locus_tag="PLB46_14815"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRF77847.1"
                     /translation="YIAGIIRTYSWEVLPRIPVKLKVLSPSGKEFKSLKKTLDDQGGF
                     ETGIVLPKTATTGTYTVELYTSNDVLLTTSYISVEEFMPDRIKVSSNLNKNEYYPGDE
                     VKTSIKADNLYGTPAANRNWEAEINLNRSYFSSKKYNNYNFQISGGETYLSTQTTEGT
                     TNDDGEADVAFVIPSEYVNIGLLNGRVMTTVFDESGRPVYDVKDFKVITQNILLGIGD
                     FDYYTKTKQSIKIPLIALDKDDKLSSSDAKVEIIKHEYETVIEKNRTYYTYRSNKVEK
                     LQSTQTITIKGENTAVWFTPQLSGEYEVRVKFPNSGTYVSQHFYAYGWGNTQSNSFEV
                     DNEGNINIVADKENYNVGDEANLLFTAPFNGKLLVTVERNKVFEYYYLDTEKKSASLK
                     LKMDNSFVPNVYVTATLFRPADDSQLPLTVAHGITNLKVDNLAKKMQVTVTHDQNSRS
                     NKKQTIQIKAAPNAEVTVAVVDEGILQIKNFQTPDPYNYFYMQHALGVMSYDLYAYLY
                     PEIYLKKLLSGGDGGDAGARLNPMTANRVKLVSYWSGILKTDGSGKAKFDIDIPQFSG
                     DLRVMVVAYKNDMFGSSETHMKVADPIVVTTSLPRFLSPGDTITVPVTLANTTGKNAS
                     GKINISAEGPIKVAGSNNIDFSASANKESRVEFKMVATGQTGNAKVKVKVQALNETFT
                     DEIEIGVRPPASLQKYSGSGYVEGNKTQTINLTNNFVGDGAKAKLVVSRSPIVQFTDH
                     LEYLINYPHGCVEQITSSVFPQIYYGDLVKEIYGKETKDLNPSYNVQEGIRILESMQM
                     YNGALMYWPGGGYESWWGTIYATHFMYEAKKAGYDVDDKVLNRSYGYMKNMLKQKKTF
                     VYYYNSNLSKEMAAKEVFYSMYVLALAGKAEISTMNYYKAN"
BASE COUNT          934 a          436 c          539 g          783 t
ORIGIN      
        1 gtatattgca ggtattatca gaacttattc ctgggaagta ttacctagaa ttccggttaa
       61 gctaaaagta ttatctcctt cgggaaaaga atttaaatcg ctgaaaaaaa cgctggatga
      121 tcagggtgga tttgaaaccg gaattgtatt acctaaaact gcaactacag gaacctatac
      181 agttgagttg tatacctcta atgatgtatt actcacaacc agttatatca gcgtagagga
      241 gtttatgccg gacagaatta aggtatcctc taatctgaat aaaaacgaat attacccagg
      301 tgatgaagta aaaacttcaa ttaaagcaga taacttatac ggcactccgg ctgctaatcg
      361 caactgggaa gctgaaatta atttaaatcg ctcctatttc agttcaaaaa aatataataa
      421 ctataatttc cagatttcag gtggcgaaac gtatttgtcg acacaaacaa cggaaggcac
      481 aaccaacgat gatggtgaag ccgatgtagc ttttgtaata ccatcagaat atgtgaatat
      541 aggtttatta aacggacgtg taatgacaac tgtatttgat gaatctggtc gacctgtata
      601 tgatgttaag gattttaagg ttattactca aaatatctta cttggtattg gcgatttcga
      661 ttattatacc aaaacaaaac aatcgattaa aattccgtta attgcattgg ataaggatga
      721 taaactttca agttcagatg ctaaagtgga aattatcaaa catgaatacg aaacggtaat
      781 tgaaaaaaac agaacttatt atacctaccg ttccaataaa gttgaaaaat tacaaagcac
      841 tcaaaccatt accataaagg gtgaaaacac tgctgtgtgg tttacacctc agttgtccgg
      901 tgaatatgaa gtacgcgtta aatttcctaa ttccggcact tatgttagtc agcattttta
      961 cgcctatggc tggggaaata cacaaagcaa ttcgtttgag gtagataatg aaggtaatat
     1021 taacatcgtt gccgataaag aaaattacaa tgtcggtgat gaagctaatt tattatttac
     1081 cgctccattt aacggtaaac ttttagttac ggtagaaaga aataaagtat ttgaatatta
     1141 ttatctagac acagaaaaga aatcagcatc tttaaaatta aaaatggata acagttttgt
     1201 gccgaatgtg tatgtaactg caactttatt ccgtccagcc gacgacagtc aactaccatt
     1261 aacagttgcg catggtataa ccaacctgaa agtagataac cttgctaaaa aaatgcaagt
     1321 aacggttaca catgatcaaa attcgcgcag taataaaaaa caaaccattc agattaaagc
     1381 tgctccaaat gcagaagtaa ctgttgcagt tgtggatgaa ggtattttgc agattaaaaa
     1441 tttccaaacg ccggaccctt acaactattt ttatatgcaa catgcattag gtgtaatgag
     1501 ttacgattta tatgcgtatt tgtatccgga aatttattta aagaaattat taagtggtgg
     1561 tgacggtggt gatgcaggag cgcgtttaaa tccaatgact gccaatcgcg tgaaactggt
     1621 ttcatactgg agtggcattt tgaaaactga tggatcaggc aaagcgaaat tcgatattga
     1681 tattccacaa tttagtggcg acctgcgtgt tatggtggtc gcttataaaa acgacatgtt
     1741 tggcagtagc gaaacgcata tgaaagttgc tgacccaatt gttgtgacta cttccttacc
     1801 aaggttttta agtccgggcg atactattac agtacctgtt acgttggcca atacaaccgg
     1861 taaaaatgca tcaggtaaga taaatatcag cgccgaaggc ccgataaaag ttgccggtag
     1921 taataatatc gattttagtg cttctgcaaa taaagaatca cgtgtggaat ttaaaatggt
     1981 ggcaacaggg caaaccggaa atgctaaagt aaaagtaaaa gtgcaggcat tgaacgaaac
     2041 gtttaccgat gagattgaaa tcggtgttcg tcctcccgca tccttacaaa aatattcagg
     2101 ctcagggtat gtggaaggta ataaaacaca aactatcaat ctcaccaata attttgttgg
     2161 tgatggtgct aaagcaaaat tggtagtcag ccgttcacca attgttcaat ttactgacca
     2221 tctggaatat ttgattaatt atccgcatgg ttgtgtcgag caaattacct cgagtgtatt
     2281 ccctcaaatt tattatggtg atttggtgaa agaaatatat ggtaaggaga caaaagattt
     2341 aaatccttct tacaatgttc aggaaggtat tcgaatattg gaaagcatgc aaatgtataa
     2401 cggcgcattg atgtattggc ctggtggtgg atatgaaagt tggtggggaa ctatttatgc
     2461 tacccatttt atgtatgaag ccaaaaaagc aggttatgat gtagacgata aagtgttaaa
     2521 tcgctcatat ggttacatga aaaatatgct taagcaaaag aaaacttttg tgtattacta
     2581 caattcgaat ttaagtaaag agatggctgc caaagaagta ttttacagta tgtatgtatt
     2641 agctctcgca ggtaaagcag aaatctctac catgaattat tacaaagcca at
//
LOCUS       DAORKW010000512         2688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2243_length_2688_cov_4.366122, whole genome shotgun sequence.
ACCESSION   DAORKW010000512 DAORKW010000000
VERSION     DAORKW010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2243_length_2688_cov_4.366122"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLB46_14820"
     CDS             <1..189
                     /locus_tag="PLB46_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77848.1"
                     /translation="HVSKGTSANKSIDLSKYPQTKSAIHFSYKEKLPTTIIKKIIKAR
                     LVENIAKEKIKAEKKAKK"
     gene            214..1731
                     /locus_tag="PLB46_14825"
     CDS             214..1731
                     /locus_tag="PLB46_14825"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HRF77849.1"
                     /translation="MRNWLLALSILFIGQAFAQAPAFPFPQHTTYTAGSIKPNVVTQS
                     GMDKVVKQFYNKWKTRYLHSGCEPGHYYVYYNFEGGADPANAICVSEGQGYGMMITAY
                     MAGYDPNAKNYFDGLYYYYKAHPSIINPTLMAWQQITGCENVDGADAATDGDLDIAYA
                     LLLAHAQWGSDGAIDYLNEAINLIDAIYTSEINNNNQTVKLGDWVSGSVSPMSYSTRS
                     SDFMPDHFKSFYAATGDNHWLLTSDRCYTITDAIQTNYSTSTGLMPDFIRHTNTAPDP
                     AAPNFLESTNDGDYYYNACRFPWRLATDYLITGDTRAYDALAPINSWIKIKTSSLPAN
                     IKSGYDIETGNMLAGANYNDLAFQAPFAVSAMIDGSNQAWLNSLWNYMKTVNINRDGY
                     YGNSIKLLSLIVISSNWWAPDEALFRTSAPAINSAISIYPNPASERLLHIQLTNNDIT
                     DAQYAVFGLDGRKTSVNGTFDAASTTIDIADLPSGTYLIVINTISGVYSKTFVKI"
     gene            complement(1734..2186)
                     /locus_tag="PLB46_14830"
     CDS             complement(1734..2186)
                     /locus_tag="PLB46_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRF77850.1"
                     /translation="MQDVILNKLRHYCAYQERCHEEVRTKLLSLKVYGDALEEIINKL
                     IEEDFLNEERFAKAYAGGKFRMKKWGRGKIVQGLKARKISDYCIKQGMTEIPDDDYQK
                     TLESLVKSTIKKYHTGNVFETKSKTANYLISRGYESELVWDAIQRHLK"
     gene            complement(2256..>2688)
                     /locus_tag="PLB46_14835"
     CDS             complement(2256..>2688)
                     /locus_tag="PLB46_14835"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HRF77851.1"
                     /translation="NAIVGYNKWSLRVMARLYNLSDGYPSFGPDGADDKTEFHIDLIH
                     VGRGQLLLRSLFGWLYVGIPHGICLYVRFIGTFVLMFLAWWVVLFTGKYPENWHRFNV
                     GSFRWATRVSLYLMWLSKDYPPFKGAPDEMDEADKPLDQVA"
BASE COUNT          873 a          508 c          474 g          833 t
ORIGIN      
        1 catgtttcga aaggcacttc agctaataaa tcaatcgatt taagtaaata tccgcaaaca
       61 aaatcggcta tccatttttc atataaagaa aaattgccga caacaattat taaaaaaatt
      121 attaaggcac gcctcgtaga aaatatagcc aaagaaaaga ttaaagcaga aaaaaaagca
      181 aaaaaataat ttttcatttt aaattctact aatatgcgta attggttgct tgcattatca
      241 attttattta ttggacaagc ttttgcgcaa gcacctgctt ttccatttcc gcaacatact
      301 acttatactg cagggtcgat taaacctaat gtagtaacac aatccggtat ggacaaagtg
      361 gttaaacaat tttataataa atggaaaacc cgttatttac atagtggttg tgaacccggg
      421 cattattatg tgtattataa tttcgaagga ggtgccgacc cggctaatgc tatttgcgtg
      481 agtgaaggac aaggatatgg tatgatgatt actgcctata tggccggtta tgacccgaat
      541 gcaaaaaatt atttcgacgg gttatattat tactataaag cacatcctag tattattaat
      601 ccaacactaa tggcttggca acaaattacg ggttgcgaaa atgtagatgg agccgatgca
      661 gctactgatg gagatttaga tattgcctac gcattattac ttgcacatgc tcaatgggga
      721 agcgatggtg caattgatta tttaaatgaa gccattaatt taatagatgc aatttatact
      781 tcggagatta ataataataa tcaaaccgta aaattgggcg attgggtttc aggaagtgtt
      841 tcacctatgt cgtactcaac caggtcaagc gattttatgc ccgatcattt taaaagtttt
      901 tatgccgcca ccggcgataa tcattggtta ttaaccagcg acagatgtta taccattaca
      961 gatgcaatcc aaacaaatta ttcgacgtca acaggattaa tgcctgactt tattcgccat
     1021 accaataccg cacctgatcc ggcagctcct aattttttag agtcgacaaa tgatggtgat
     1081 tattattaca atgcttgccg tttcccatgg cgtttggcta ccgattattt aataaccggc
     1141 gacacaagag cttatgatgc gttagcacca attaattcct ggattaaaat taaaacaagt
     1201 tcattacctg ccaatattaa aagtggttac gatattgaaa ccggaaatat gttagccggt
     1261 gctaattata acgacctggc ttttcaagct ccatttgcgg tgagtgctat gattgatggg
     1321 agtaatcaag cctggctcaa cagtttatgg aattatatga aaaccgtaaa tattaatcgc
     1381 gatggatatt atggcaacag tattaaatta ttatcactaa ttgtaatcag cagtaattgg
     1441 tgggcaccgg atgaagcgtt gtttcgtaca tctgcacctg caataaattc agcgatatca
     1501 atttatccaa accctgcaag cgaaaggtta ttacatatcc aattaacaaa taatgatata
     1561 acagatgcac aatatgctgt atttggttta gacggacgca aaacttctgt taatggcacg
     1621 tttgatgctg cttctacaac aattgatatt gctgatttac cgtcaggcac ttaccttatt
     1681 gtaataaata caataagtgg tgtttattcg aagacttttg taaaaatata ggtttatttc
     1741 aggtggcgct gaatggcatc ccaaactaat tcactttcat aaccgcggct aattaaatag
     1801 ttggcggttt tactttttgt ttcgaagaca tttccggtat ggtatttttt aatagttgat
     1861 tttactagtg attctaaagt tttttgatag tcgtcatccg gaatttctgt catcccttgt
     1921 ttgatacaat agtctgaaat tttacgcgct tttaatccct gaacaatttt acctctcccc
     1981 cactttttca ttctgaattt acctccggca taggctttgg caaatcgttc ttcgttaaga
     2041 aaatcttctt caataagttt attgattatt tcctctaaag catcaccata aaccttgaga
     2101 gataataatt tagtacgcac ttcttcatga cagcgttcct ggtaagcaca ataatgacgt
     2161 agtttattaa ggataacatc ttgcataacc gcataaaaat aaataaccgg tgcgttatgc
     2221 taccggttat tttaaaaaaa gtatgtatta tttaattaag caacctgatc aagtggtttg
     2281 tctgcttcat ccatttcatc cggcgcacct ttaaatggcg gataatcttt tgacaaccac
     2341 attaaataca aactaacacg tgttgcccac cggaaactgc caacattaaa gcggtgccag
     2401 ttttccggat atttgccagt aaataacact acccaccagg caagaaacat taatacgaat
     2461 gtaccaataa aacgcacata caggcaaatt ccatgtggaa tacccacata taaccatcca
     2521 aataacgaac gtaataataa ttgaccgcgt ccaacatgaa ttaagtcgat atgaaattcg
     2581 gttttgtcat cagctccatc aggtccaaat gaaggatagc catcagaaag gttatacaac
     2641 cttgccatga cgcgcaacga ccacttgttg taacctacaa ttgcattg
//
LOCUS       DAORKW010000513         2683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2248_length_2683_cov_5.467275, whole genome shotgun sequence.
ACCESSION   DAORKW010000513 DAORKW010000000
VERSION     DAORKW010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2248_length_2683_cov_5.467275"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1442
                     /locus_tag="PLB46_14840"
     CDS             180..1442
                     /locus_tag="PLB46_14840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77852.1"
                     /translation="MKTRSTNKLLQYSTLSTAFLIAGKVEAQTEYHEFDPYLPLTNAS
                     EVFIDVNDDGNDDFSFYFAKSWGTSSTGYAGDNFEFRAKQLGENEMMIVSLPAEAFYS
                     SFSSIYCSFPSTLGIKQLEENSIIDVFKEFGKANVMARVDQCGFYGADGEQGEWLLEG
                     GTKDYFVGFRTNDISGKYGWMRIRHYDFGDVDYVKDFYLNDAIGSGVQTPDLENMVAP
                     APDNLNLFYGGDGKLMLEFNAASDEDSLQEYRVILRNTIYTSELTATICETTLPGNYI
                     TVNKTGISTYVADLSTLTTDWVGNTLEEGDGISAYVYSKFNYPVTMLNGLSPESNERT
                     LSDDVAIHNNVNNETQVWLHDNVLHIIATNNNIEQIVIYDILGRIVYAEPLSESQNHI
                     SIPNQLNQGIYVVSMVTSDGVISRQIKL"
     gene            1447..2346
                     /locus_tag="PLB46_14845"
     CDS             1447..2346
                     /locus_tag="PLB46_14845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77853.1"
                     /translation="MHPTTQTRLMHYSLMAGAITVAQKSNAQIIYTDIEPDILLYQYV
                     NDSEGWGYVDLNDDDITDVGVLFEVGYNCGYCPYWVDFRVNVSTPNEAAVAFDGPCTL
                     STAYSDGTCYVPGRWLTNFLVEGQEINPEGEFLNNAPELYDFNCGAYGGQCVQGFFRS
                     EYSATDDQFIAIRLIEMDTNYCWIRLRWEGDKLYLKDYTCNLNVGEGLIIDIPEPLPV
                     NIENETWQPSIITTNNSIQIQSNIIPENGIIQLVDMQGRIVYATAWNQKKHIIETDLP
                     SGLYILTIQDASHNYSQQIALPF"
     gene            complement(2410..>2683)
                     /locus_tag="PLB46_14850"
     CDS             complement(2410..>2683)
                     /locus_tag="PLB46_14850"
                     /inference="COORDINATES: protein motif:HMM:NF015881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal L9 C-terminal domain-containing
                     protein"
                     /protein_id="HRF77854.1"
                     /translation="RKLLEKIQEVTAQLQANTIKVGAKVGASDKIFGSITNVQLAEAI
                     KKQIGLVVDRKKIVLPDEVKTLGSFTAHIALDKDHNIPVNFEVIEG"
BASE COUNT          850 a          436 c          520 g          877 t
ORIGIN      
        1 gggatagatg cctgctcgtc gcgcctccaa actcgaccca atcgaagcac ttcgctacga
       61 ataattttgc tttcgctaat tcatacatag gtttcgctta gtggaaaccg tcatttcaca
      121 catttccggt tttctggtaa tttgttgtaa attagtttga cagcataaat caatatatta
      181 tgaaaacccg cagcacaaac aaattattac agtactcaac tttatctact gcttttttaa
      241 tagcaggaaa agtggaagca caaactgaat atcacgaatt tgacccttat ctaccattaa
      301 ccaatgcatc agaagtattt attgatgtaa atgatgatgg caacgatgat ttctcttttt
      361 actttgcaaa aagctggggt accagttcaa caggatatgc cggagataat ttcgaatttc
      421 gcgccaaaca attaggcgaa aatgaaatga tgatagtatc attacctgct gaagcatttt
      481 attcatcatt ttcttctatt tattgttcat tcccttcaac cttaggtata aaacaattag
      541 aagaaaattc cattatcgat gtatttaaag aatttggcaa agccaatgta atggcacgtg
      601 ttgatcaatg cggattttat ggagcagatg gtgaacaagg tgaatggtta ctcgaaggtg
      661 gaacaaaaga ttattttgtt ggattcagaa caaatgatat aagtggtaaa tatggatgga
      721 tgcgcattcg tcattacgat tttggcgatg tagattatgt aaaagatttt tatctcaacg
      781 atgcaattgg cagtggtgtt caaacgcccg accttgaaaa tatggttgct cctgcacctg
      841 acaatttaaa tttattttat ggtggagatg gcaaattgat gttggaattt aatgcagcaa
      901 gcgatgaaga tagtttacag gaataccgcg ttattttgcg caataccatt tataccagtg
      961 aattaacagc aaccatttgt gagactactt tgcctggtaa ttacattacc gttaataaaa
     1021 ccggcatttc aacttatgtt gcggacctca gtacactaac taccgattgg gtaggaaata
     1081 ctttagaaga aggagatggt attagtgctt atgtatatag taaatttaat tatccggtta
     1141 ctatgttaaa tggcttgtcg cctgaatcga atgaacgcac attgtctgat gatgtggcta
     1201 ttcataataa tgtgaacaac gaaacgcagg tatggctgca tgataatgta ttacatataa
     1261 ttgcaacaaa taataacatt gaacaaattg taatttacga tatactcggt cgtatagtat
     1321 atgcggaacc tttaagtgaa agtcaaaatc acatcagtat tcccaatcaa ttaaatcagg
     1381 gtatatatgt cgtttctatg gttacgtcgg atggtgttat tagcaggcaa ataaaattgt
     1441 aatattatgc atcctactac ccaaacacgc ttgatgcatt acagtttaat ggctggtgca
     1501 ataactgttg cacaaaaatc aaatgcacaa attatttata ctgatattga accggatatt
     1561 ttattatacc aatacgtaaa tgattcagag ggctggggtt atgttgattt gaatgatgat
     1621 gatattactg atgttggcgt attatttgaa gtaggatata attgcgggta ttgcccgtat
     1681 tgggtcgatt tcagggtgaa tgtcagcaca ccaaacgagg ctgcagttgc atttgatggg
     1741 ccatgtactt tgtctaccgc ttacagtgat ggcacctgct atgtgccagg tagatggtta
     1801 accaattttt tggtagaagg acaggaaatt aatcccgaag gtgaattttt aaataatgca
     1861 ccagagttat atgattttaa ttgtggtgca tatggtgggc aatgtgttca aggttttttc
     1921 agaagtgaat atagtgcaac agatgatcaa tttattgcta tccgattaat tgagatggat
     1981 actaattatt gttggattcg tttgcgatgg gagggggaca aactgtattt aaaagattat
     2041 acctgcaatt taaatgtagg tgaaggttta attattgata ttcctgagcc attgccggtg
     2101 aacatcgaaa atgaaacatg gcaaccttca attattacaa caaataatag tattcaaatt
     2161 caatcgaata ttattccgga gaatggtatt attcagttag tagatatgca aggccgaatt
     2221 gtatatgcaa cggcatggaa tcaaaaaaaa catattattg agactgactt accttcaggt
     2281 ttatacatct taacaataca ggacgcttcc cataattatt cgcaacaaat tgcattaccg
     2341 ttttaaaaat aaaaccctca caaattaaat tgtgagggtt tttactatat attctccaac
     2401 cttttatgct tatccttcga taacttcaaa gtttaccgga atgttgtggt ctttatctaa
     2461 cgcaatgtgc gcagtaaatg aacctaaagt tttcacttca tcaggaagaa caattttctt
     2521 tctgtcaaca actaatccaa tttgtttttt gattgcttcg gctaattgaa cgttggtaat
     2581 agaaccaaaa attttatcgc ttgcacctac tttagcgcct actttaatgg tatttgcttg
     2641 taattgcgcg gtaacttcct ggattttttc gagtaattta cgc
//
LOCUS       DAORKW010000514         2681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2254_length_2681_cov_5.500381, whole genome shotgun sequence.
ACCESSION   DAORKW010000514 DAORKW010000000
VERSION     DAORKW010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2254_length_2681_cov_5.500381"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLB46_14855"
     CDS             <1..224
                     /locus_tag="PLB46_14855"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRF77855.1"
                     /translation="HNAKPKVPAHRVVNRNGLLTGKAHFNPPEKMVELLEKEGIEVKN
                     DQVVNFKLLYWDPAVELEDPEMPSPTKKK"
     gene            complement(268..2487)
                     /locus_tag="PLB46_14860"
     CDS             complement(268..2487)
                     /locus_tag="PLB46_14860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF77856.1"
                     /translation="MNIASCTYGHLYKQYLLLGLIYILSVHCAFTQVPGMYPDIEWQK
                     CNGGSAMEWAYNMVPTADGGVVCVGRTKSTDGEVTGFVGEEDCWITKLDSNGNLMWQK
                     TLGGLEFDHAASVVEAADGSIYVAGYTGSIDGDFVSSHGSNDAFVAKISSTGLLLWIK
                     CYGGPGSDTAIKIDFISENKISFLGTSGTVGGDVTSNYGMSDLWLVAIDSAGAILSQK
                     SYGGSDSDYGKDMVQTDDGGYAIISSAQSNDIDATYNHGSWDYWFLKIDSNGIIQYQN
                     SLGGSGEDMPKAITKSLSNGYYINGQSASKNYDVTGHHGVDWTHDFWVVHIDSMGELI
                     WQKSLGGFDDDNGESIFVQDENNIIVGGSSHSHDGDVIGNHGSPGYSDYWMICLDSLG
                     SINWQKCFGGTLTDYGMAYTLIPGNGFMATGMGVSNNFDISGNHGYGDFWTIKLSEPC
                     LHTTYYSDYDGDTYGNPFNAINSCEDTLIGYVLNNLDCNDSNFVINPDGIEICNDLDD
                     NCNTLYDEGFALNVFFADADGDGFGNTAIFIVSCLTEIPGYVELGNDCNDTTLIINPD
                     ATEICNGIDDNCNGLIDDGFAMNTYYFDADGDLFGDHENDTTACVAAISGFVADSTDC
                     DDSNPLIFPGAEELLNGIDDNCNLLIDEGVAINTINTMPFNVFPNPANNELFISFQIP
                     VNGNVSLYDLMGNLVLIKPIVEINFLKIDIALLASGNYFLQFNSNAGSEFLGIIQKE"
BASE COUNT          898 a          572 c          429 g          782 t
ORIGIN      
        1 gtcacaacgc caaaccaaag gtgcccgcac acagagtagt gaacagaaat gggttactta
       61 ccggtaaagc acattttaat ccgccggaaa aaatggtcga attattagaa aaggaaggta
      121 tagaagttaa aaacgaccaa gtagttaatt ttaagttgtt gtattgggac ccggctgtcg
      181 aattggaaga ccctgaaatg ccttcaccta caaaaaagaa gtgaaaaata ggcaaaacat
      241 acattcagtg caagtctgag aactaattta ttctttctgt attataccca aaaattcact
      301 gcctgcattt gagttaaatt gtaaaaaata gtttccggaa gcgagtagtg caatatcaat
      361 tttaaggaaa ttaatttcaa ctattggttt aattaaaacc aaatttccca ttaaatcata
      421 gagtgaaaca ttgccattta ccggaatttg aaatgatatg aataattcat tatttgcagg
      481 attcggaaaa acattaaacg gcattgtgtt aatggtattt attgcaacgc cttcatcaat
      541 taacagatta caattatcat caattccatt gagcaattct tctgcgcctg gaaatattaa
      601 tggattggaa tcatcgcaat cggtgctatc tgctacaaaa cctgaaattg ctgctacaca
      661 agcagtggta tcgttttcgt ggtctccgaa taaatcgcca tcggcatcaa aataataagt
      721 attcattgca aagccatcat caattaatcc gttgcaatta tcatcaattc cattgcaaat
      781 ttctgttgcg tcggggttaa ttattaaggt agtatcgttg caatcattac ccaattcaac
      841 atatcctgga atttctgtta aacaggaaac gatgaaaata gcagtattcc caaatccatc
      901 accatctgca tctgcaaaaa atacattcaa tgcaaatcct tcatcatata atgtattaca
      961 attatcgtcg agatcattac aaatttctat tccatcagga ttaattacaa aattggaatc
     1021 gttgcaatct aaattgttaa gcacataacc tattaaggta tcttcacaac tatttatagc
     1081 attaaatgga ttgccataag tatcaccgtc ataatcagaa taataagtag tatgcaaaca
     1141 aggttctgaa agttttattg tccaaaaatc gccataacca tggttaccgg aaatatcgaa
     1201 attatttgaa acccccatgc ctgttgccat aaaaccattt ccaggtatta gggtatatgc
     1261 cattccataa tctgtaagag tgcctccgaa acatttttgc caattaatag aacctaacga
     1321 gtctaaacat atcatccaat aatcggaata tccgggagat ccgtgattgc caattacatc
     1381 accatcatgt gaatgtgaac ttccaccgac aataatatta ttctcgtctt gaacaaatat
     1441 tgattcacca ttatcatcgt caaatccgcc taatgatttt tgccaaatta attctcccat
     1501 agagtcgata tgcaccaccc aaaaatcatg tgtccaatca acaccgtggt gaccggtaac
     1561 atcataattt tttgaagctg attgaccgtt aatgtaatag ccattactta aacttttagt
     1621 aattgctttt ggcatatcct cacctgaacc ccctaatgaa ttttggtatt gaataattcc
     1681 attgctatct atttttaaaa accaataatc ccaactacca tggttatatg tcgcatcaat
     1741 atcattagat tgtgcggaag atatgattgc gtagccgcca tcatcggttt gcaccatgtc
     1801 ttttccataa tcgctatcgc tacctccata tgatttttgg gaaagtattg ctccggcaga
     1861 gtcaattgct accaaccaca aatcggacat gccgtaattt gaagtcacat caccacccac
     1921 tgtgccggag gttccaagaa aggaaatttt attttctgaa atgaaatcaa tttttatggc
     1981 agtgtcactc cctggcccgc cataacattt aatccagagt aacaaacctg tggatgatat
     2041 ttttgcaacg aaggcatcgt ttgaaccatg cgaactaaca aaatcgccat caattgaacc
     2101 ggtatatccg gcaacataaa tactcccgtc agcagcttca actactgatg ctgcgtggtc
     2161 gaattcaagt ccaccaagag tcttttgcca cattaaattt ccgttgctgt ctaatttagt
     2221 aatccaacaa tcttcttccc caacaaagcc tgtaacttca ccatcagttg atttggttct
     2281 accaacacaa acaacgcctc catcagctgt tggaaccatg ttatatgccc attccattgc
     2341 agaacctcca ttacattttt gccattcgat atccggatac attccgggaa cttgtgtaaa
     2401 ggcgcagtga accgataaaa tgtaaattaa cccaagtagt aaatactgtt tatataagtg
     2461 accatatgta cacgaagcaa tattcaaaat caactatttg ccaataaaga tatgtatagt
     2521 tggctcctca cgttacaaaa tcgtgtgaaa tatgcaatta agcatgtgaa atgcatgtat
     2581 aatttagtga atgtagcaaa aagcaaacaa acattgcggc atcaccaaga accaccggct
     2641 ccaccgccgc caaaaccgcc accaccaaat ccgccgaagc c
//
LOCUS       DAORKW010000515         2674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2265_length_2674_cov_4.442535, whole genome shotgun sequence.
ACCESSION   DAORKW010000515 DAORKW010000000
VERSION     DAORKW010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2265_length_2674_cov_4.442535"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2649
                     /locus_tag="PLB46_14865"
     CDS             <1..2649
                     /locus_tag="PLB46_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77857.1"
                     /translation="LKKHFSRYRNSIARYSLMGIMTLFINMSYSQVNIAMHTNNISTI
                     NDLWNIGIASMYPNQQIVDVEMTVYSEDETVLYKGNKTTIVLQPSLNISITPTSVGIH
                     HDIIDFPKNDTLQQVDASFIPNGNYVLCVSVFAHEPKVELNRSCEFITVKNSYLDSLL
                     LAARGKVPKAGIQLHSTVEIFSYIDVYTEPTPTTLQHRNTGIFLNPSVEVFGYPLNMS
                     IFYDTDSDFYYSNIPTFQFNFDTKAYQQILQERLQSQLASKLPLSPEKYQEAMTMITS
                     LEDFESITNNPYFQQEVARLDSLKVYEKYLNDSAALTLVQAQIQHLDTLSLEQMNNDS
                     LCLDSTTVMQQTQRVKDSLIAIQDSITNTIAAGKAIIEKAKRYQEILQNQALLEEQIL
                     ADSNIAQVKAYYDKFNGFDASTLSDPNLITAKLKEIDQLKKIEGFITGFESLQFGATV
                     PNYSELSISSVLLNGLNVNYAIGNCSVIAVAGRINDNSTFFKPDWEQRTFSKLYVAGF
                     EQQITTNSKYGLFVLQSDFSDQDSLSFYNFLEKNNTIAGKFSTGFFKNKLKAEGEIAI
                     SYAENKDIIGFEEVGEEVANVPNFWLLQSLVQKDDLASGTFTDKAAILSLSSAFFQNK
                     TTVAITGRYVGSGFFTPGNPFLLNDLMNVEFGVDQSLFKNKLQLSAHVIKNQDNLEQL
                     KEVTTSYYNLRAGIKLNVPKYPMVSIDYLPNVIINEFDQIQVNTLSAITAYNYRLGNI
                     PCLISFNYMDIQTLSAENDSTNFNSRYYNMLNNFNFKKFDIQTGFNYNEAITVTEAVI
                     FSIYSVGTRFSATKWMDVYTNFQLTGNEHNNFEPGGQLELNFSPLATMKFKTGFYVYP
                     ESTLEYMTNIQNISNTSIYISATYNF"
BASE COUNT          928 a          442 c          454 g          850 t
ORIGIN      
        1 ttgaaaaaac atttttcaag atacaggaac agtattgccc gttattcttt aatgggaatt
       61 atgaccttat ttattaatat gagttatagt caggtaaata ttgcaatgca tacaaacaac
      121 atttcaacga taaatgattt gtggaacatt ggtattgctt caatgtatcc taatcaacaa
      181 attgttgatg tagaaatgac ggtatatagc gaagatgaga ctgttttata taagggtaac
      241 aaaacaacta tcgtattaca accttcactt aatatttcca ttacacctac atcagtaggc
      301 attcatcatg acattattga ttttccgaaa aatgatacat tacaacaagt ggatgcatcc
      361 tttattccta acggtaatta tgtattatgt gtttctgttt ttgcacatga accgaaggtg
      421 gaattaaatc ggagctgtga gtttatcacc gtaaaaaata gctatctgga tagtctgtta
      481 cttgcagcta gagggaaagt gccaaaagcc ggcattcaat tacatagtac ggtagaaata
      541 ttttcatata ttgacgtgta tacggagccc acgccaacaa cactgcaaca ccgaaatacg
      601 ggtatattct taaacccttc agttgaagtt tttggatatc cattaaatat gtccatattt
      661 tatgatactg atagtgattt ttactatagt aatataccaa cctttcaatt caattttgac
      721 accaaggctt accaacaaat attacaagaa agattgcaaa gtcagctggc ttcgaagtta
      781 ccactttcgc ctgaaaaata tcaggaagcg atgaccatga taacttcatt ggaggatttt
      841 gaaagcatta ctaacaaccc ttattttcag caagaagtag caaggctaga tagtttaaaa
      901 gtatatgaaa aatacttaaa tgattctgct gcgttgacat tagtgcaagc tcaaattcaa
      961 catttggaca ctttatcctt ggagcaaatg aataacgatt ctttatgttt agatagcacc
     1021 actgtaatgc aacaaacgca acgtgtaaag gattctttaa ttgcgataca agattcaatt
     1081 accaacacca ttgctgccgg aaaagcgatt attgaaaaag cgaaacgata tcaggaaatc
     1141 cttcaaaatc aagcactttt ggaggaacaa atattggcag actcaaatat tgcacaagta
     1201 aaagcatact acgataaatt caacggtttt gatgcatcaa cattgagtga tcctaaccta
     1261 attacggcta agctgaaaga gattgatcaa ctcaaaaaaa ttgaaggatt tattaccgga
     1321 tttgagtcat tacaatttgg tgctaccgta ccaaattact ctgaattgtc aatttccagt
     1381 gtgttgctta atggacttaa tgtaaattac gccataggta attgttcagt gatagctgta
     1441 gctggtcgta taaatgataa ttctaccttt ttcaaaccag actgggaaca acgcactttt
     1501 agtaaattat atgtggcagg atttgagcaa caaattacta caaatagtaa atatggtttg
     1561 tttgtcttac aatccgattt tagcgaccaa gactctttgt ccttctataa tttccttgaa
     1621 aaaaataata ccattgccgg taaattttca actggatttt ttaaaaataa actcaaggct
     1681 gaaggtgaga ttgcaatctc ttatgctgaa aataaagata taattggttt tgaagaagtt
     1741 ggggaggaag ttgctaatgt ccctaatttt tggttattac aatccctagt gcaaaaggat
     1801 gatttagctt ccggcacttt tactgataaa gcggcaatac taagtctctc cagtgcattt
     1861 ttccaaaaca aaactacagt tgctattacc ggtaggtatg ttggttcagg attttttacc
     1921 ccgggtaatc cttttttgct caacgatttg atgaatgttg aatttggagt cgatcagtcg
     1981 ttgtttaaaa acaaattgca actcagtgcg catgtaatta aaaaccagga taatctggag
     2041 cagttaaaag aagtgactac ttcttactat aatctgagag cagggataaa attaaatgtt
     2101 ccaaaatacc cgatggtttc tattgattat ttaccaaatg tgataatcaa tgaatttgac
     2161 caaattcaag taaataccct ttcagcaatc actgcataca attataggtt aggcaatata
     2221 ccatgtttga tttcctttaa ttatatggac atccaaacac tgagcgcaga aaacgatagc
     2281 accaatttta atagccgtta ttataatatg cttaataatt tcaattttaa aaaattcgat
     2341 atacagacag gttttaatta taacgaagct attacagtta cagaagcggt catatttagt
     2401 atttacagtg taggcaccag atttagtgca actaagtgga tggatgtgta taccaatttt
     2461 caactaacag gtaatgagca caacaatttt gaaccaggag ggcagttgga actcaatttt
     2521 tctccattag caacgatgaa atttaaaacc gggttttatg tataccctga atcaacttta
     2581 gaatatatga ccaatattca aaatatttca aatacatcaa tttacatttc agcaacctat
     2641 aatttttaat tatgaaaaaa gttgtgttaa ttat
//
LOCUS       DAORKW010000516         2658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2281_length_2658_cov_4.071072, whole genome shotgun sequence.
ACCESSION   DAORKW010000516 DAORKW010000000
VERSION     DAORKW010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2281_length_2658_cov_4.071072"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2170)
                     /locus_tag="PLB46_14870"
     CDS             complement(<1..2170)
                     /locus_tag="PLB46_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF77858.1"
                     /translation="MISLTAAAQEVQWASEVVRFSTEYTRKQFAAKQVLGKPDKLPAF
                     GESAVAWAPSTPDNPAGEFIHVRFANPQKVQQIGIGESNCPGSIKEVILYSTTGKKYS
                     VYENLNIKPEFTTGGRIFRVFIPLTDYEVSEVRVVLNTKAIPGMNQIDCIGISNSEVP
                     IKATVNELQYAKPIPDPENLGVEVNSDADDMFAFIAPDGKTLYFARKNYHENTGGALR
                     DDIYVSKWANNKWTKAINIGFPLNNEQHNYVSWISSDGSTLLLANNYEKVKQDISISK
                     KTPDGWSFPKALKINDFYNDNEFSCYHMNTEGNVILMAIERPDSYGDMDIYVSFLQSN
                     KVWTEPKNLGMTINSAATEGSIFIASDNRTIYFATNGKSGYGGYDMFMSKRLDDTWLN
                     WTEPVNLGNKINSEGHDYYYTIPASGDYAYFSSTKNSYGKADLFRIPLPVEAQPDPVT
                     LLKGKIIDAETKQPINADVAFGGLVSEEPKGITTSTNGNYQIIIPEDNYNVTIKKPGY
                     FPVMTTIDEDLDFDEIDFNVEDPVATIKHEIKTELKADLKENKITNEEILIELNKRIN
                     EEFSDTTSTDKEAIVKEIADELTGTKDTLYAEVTADVELIPIKAGTIIQLDNIFFEAN
                     KSDLKPESSASLDELVEFLKSNPNIYVEIGGHTNGLPNDAFCQKLSNDRAKNVVDYLT
                     AKGITPEHLTWKGYGKTQPIADNATAAGRKKNQRVELKIIK"
     gene            complement(2239..>2658)
                     /locus_tag="PLB46_14875"
     CDS             complement(2239..>2658)
                     /locus_tag="PLB46_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77859.1"
                     /translation="FVIYATIASNLERNRWRAVMNFERKRVRVMRFDGYLLMACIVLF
                     IITILKPEIANLPPHKWLLATIDKIYQTAILKYIIGFFAFFYMLNIIFRGIKASDEVF
                     SILFNRKRKTQQHQMPGNDGGFTEYEEVTDDTTTLPK"
BASE COUNT          759 a          515 c          480 g          904 t
ORIGIN      
        1 ctttaatgat ttttagttcc acccgctgat tttttttgcg gcctgcggct gtggcattgt
       61 ccgcaatagg ttgtgttttg ccatatccct tccaggtaag atgctcaggt gttatgccct
      121 ttgcggtgag gtaatccaca acatttttag cccggtcgtt ggataatttc tgacaaaatg
      181 catcatttgg aagaccattg gtatgaccgc caatttctac ataaatattg ggattactct
      241 ttaaaaattc aaccagttca tcgagcgaag ccgaactttc cggttttaaa tcagatttat
      301 ttgcttcaaa aaatatattg tcgagctgaa taatggttcc tgctttaatt ggaatgagtt
      361 ccacatcggc agttacttcc gcatataaag tatctttagt gccggttaat tcatctgcaa
      421 tttcttttac aatagcttcc ttatcagtag acgtagtatc agaaaattct tcattaattc
      481 ttttattcaa ttcaatcaga atttcttcgt tggtgatttt attttcttta aggtccgctt
      541 ttaattcggt tttaatttcg tgtttaatgg tggcaacagg gtcttcaaca ttaaaatcta
      601 tttcatcaaa atctaaatct tcatcaatag ttgtcatcac aggaaaatac cccggttttt
      661 tgatggtgac attgtaatta tcttcaggga taataatttg ataattgccg tttgtagaag
      721 tagtaatacc ctttggttct tcgctcacca aacccccgaa ggcaacatca gcattaatcg
      781 gttgttttgt ttctgcatca ataatttttc ctttcagcaa ggttacagga tcgggctggg
      841 cttcaacagg aagtggtatc cgaaataaat ccgctttacc gtacgaattt tttgttgagg
      901 aaaaatatgc ataatcgcca cttgcaggaa tggtataata ataatcgtgg ccttcactat
      961 ttattttgtt gcccagatta accggctcgg tccagttcaa ccaggtatca tcgaggcgtt
     1021 tgctcataaa catatcataa ccgccataac cgctttttcc attggtggca aaataaattg
     1081 ttctattgtc ggatgcaata aatatactgc cttctgtagc tgcggaatta atggtcatac
     1141 ctaaattttt aggctcggtc cacaccttat tactttgtaa aaaactcaca taaatatcca
     1201 tgtcgccata agaatcggga cgttctatgg ccattaaaat tacattacct tcggtgttca
     1261 tgtggtaaca ggaaaattcg ttgtcgttat aaaaatcatt aatttttaat gctttcggaa
     1321 acgaccatcc atcaggtgtt ttttttgaaa tagaaatatc ctgttttact ttttcgtagt
     1381 tgttagcgag taataaagtg ctgccatcag aactaatcca tgatacataa ttatgttgtt
     1441 cattatttaa tgggaaaccg atattaatcg ctttggtcca tttattattt gcccatttgg
     1501 aaacataaat atcatcacgc aaagctccac cagtattttc atgataattt tttctggcga
     1561 aatacaatgt tttaccatca ggtgcaataa atgcaaacat gtcatctgca tcgctattta
     1621 cttctacccc caaattttcc gggtcgggga tgggtttggc atattgcaat tcatttacgg
     1681 ttgcttttat tggcacttca ctgtttgaaa taccaataca atcaatttga ttcatgccgg
     1741 gtatggcttt ggtatttaaa accacacgta cttcgctcac ttcataatcg gtaagcggaa
     1801 taaatacccg aaatattctg cctccggtag tgaattccgg tttgatattc agattttcgt
     1861 aaacactgta ttttttgccg gtggtgctgt aaagaattac ttctttaatt gagcctggac
     1921 aattactttc tccaatacca atttgttgca ctttttgtgg attggcaaaa cgcacatgaa
     1981 taaactcacc tgccggatta tcgggagtgc ttggtgccca tgctacggcg ctttcaccga
     2041 aggctggcaa tttatccggt ttgcccaaaa cttgtttggc agcaaactgt ttgcgggtat
     2101 attctgtcga aaaacgaaca acttccgatg cccattgcac ttcctgtgca gcagcggtga
     2161 gggatatcaa acaaaatagc catataaaat gaaaacgcaa tgttgtaaat tttgacttaa
     2221 acgaaaactg tgtgtgcatt attttggcag ggtagtagtg tcgtcggtaa cctcttcata
     2281 ttctgtaaaa ccaccatcat ttcccggcat ttgatgttgc tgtgttttac gtttacgatt
     2341 aaataaaatg gaaaacactt catcagacgc tttaatgcct ctgaaaatga tattaagcat
     2401 ataaaaaaag gcaaaaaatc cgatgatata ttttagaatt gctgtttgat aaattttatc
     2461 aatcgtagct aataaccatt tatgcggagg taaattcgca atttccggtt ttaatatcgt
     2521 gatgataaat aaaacgatac atgccatcaa taaatagcca tcaaatcgca ttactctaac
     2581 gcgtttgcgt tcaaaattca tcactgcccg ccaacgattg cgttctagat tgctggcaat
     2641 agtagcatat attacgaa
//
LOCUS       DAORKW010000517         2648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2293_length_2648_cov_4.614346, whole genome shotgun sequence.
ACCESSION   DAORKW010000517 DAORKW010000000
VERSION     DAORKW010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2293_length_2648_cov_4.614346"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PLB46_14880"
     CDS             complement(<1..657)
                     /locus_tag="PLB46_14880"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRF77860.1"
                     /translation="MKKIAVMTSGGDSPGMNACIRAVVRTGIYNGMEVYGIRRGYEGM
                     IDGDFYKMESHDVSNIIQRGGTILKTARSQRFMTPEGRKTAYDNLKLHGIEGFVAIGG
                     DGTFRGATVFEKEYGITCVGVAGTIDNDLFGTDYTLGFDTAINTAIDCIDKIRDTAIS
                     HNRLFFVEVMGRDAGFIALSCGIAGGAEAIVIPETETNIDNLISILQNGWQHQKSSAI
                     V"
     gene            complement(687..1628)
                     /locus_tag="PLB46_14885"
     CDS             complement(687..1628)
                     /locus_tag="PLB46_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRF77861.1"
                     /translation="MPGETILLIGASGQIGAELTMLLRSQYGNSNVVAADIKIPVGEL
                     AESGPFEELNVLDRNRIAELADKYKLTQIYNMAAMLSATGEKFPQKAWQLNMDGLINT
                     LEVAREKGIKKMYWPSSIAAFGPTTPKTDTPQITIMEPNTIYGISKQAGERWCEWYFL
                     KYGLDVRSIRYPGIISYKTEPGGGTTDYAVDIYFKAKQTGKYTCYIAENTLLPMMYMP
                     DAIKATVQLMDAPADTIKVRSSYNLAAMTFSPKMVGEAIKKHIPDFELTYDVDFRQKI
                     AETWPGSIDDSAARADWGWQHEYDLDKMTADMLKHIK"
     gene            1730..>2648
                     /gene="wecB"
                     /locus_tag="PLB46_14890"
     CDS             1730..>2648
                     /gene="wecB"
                     /locus_tag="PLB46_14890"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRF77862.1"
                     /translation="MNKILVVVGTRPNFIKIVQFKHYAALYGFDLKIVHTGQHYDENL
                     SDVFFQQLGITPDYFLDTRTANPDMQLNLIKARLNHLLNNTYKPDLMVVVGDVNSTRA
                     AAETAQILNIPIAHVESGLRSFDQSMPEEINRIVTDNLADHFFVTEQSGIDNLLEEGK
                     KAADIYFVGNTMIDALVAYDQVIAENTIMTKLDVTERAFALVTIHRPSNVDNKSALEQ
                     NIHLLQNIAIKYPVIFPVHPRTKNKLEQFQLLSLLTSNPNIRLCEPLDYFAFQKLIAS
                     CKFIVTDSGGIQEEATFRKKPCITIRPNTE"
BASE COUNT          779 a          563 c          449 g          857 t
ORIGIN      
        1 tacaattgcc gatgattttt gatgttgcca tccattttgt aaaatgctga tgaggttatc
       61 gatattggtt tctgtttcag gaattactat ggcttctgca ccacctgcaa ttccgcagga
      121 taatgcaata aatccggcat cacgtcccat tacttccaca aaaaataaac gattatgaga
      181 aatagcggta tcgcgaattt tatcaataca atctattgct gtattaattg cagtatcaaa
      241 tcctaacgta taatccgttc caaacaaatc gttatcaatt gttcctgcaa cacctacgca
      301 agtaatacca tattcttttt caaataccgt tgcaccgcga aaagtgccat caccaccaat
      361 agcaacaaat ccctcaatac catgaagctt taaattatcg taagctgttt tccttccctc
      421 aggagtcata aatcgctggg aacgagcagt ttttaaaatt gttcctccac gttgaataat
      481 attactaaca tcatgtgatt ccatcttata aaaatcacca tcaatcattc cttcataacc
      541 tcttctaata ccataaactt ccatgccgtt ataaatacct gtacgcacta ctgcacgtat
      601 acaagcattc atacccggag aatcaccacc ggaagtcatt actgcaatct ttttcatttg
      661 agcgctgtgt tttatttatt taattattat ttaatatgct tgagcatatc tgccgtcatt
      721 ttatctaaat catattcatg ttgccaaccc caatctgcac gtgcggcact gtcgtcaata
      781 ctacccggcc atgtttccgc aattttttgg cgaaaatcaa catcatacgt taattcaaaa
      841 tcaggaatat gtttttttat cgcttcaccc accatttttg gtgaaaatgt cattgctgcc
      901 agattatagg atgaacgtac tttaattgta tcagcaggtg cgtccatcaa ttgcacagta
      961 gctttaatag cgtcgggcat atacatcatt ggcaacaagg tattttcggc aatgtaacaa
     1021 gtatacttcc ctgtttgttt tgctttaaaa taaatatcaa ctgcataatc agttgttccg
     1081 ccaccgggtt ctgttttata actgattatt cccgggtatc gtatactgcg aacatctaat
     1141 ccatatttta aaaaatacca ttcacaccat ctttctccag cctgtttgct gataccatat
     1201 atggtattcg gttccatgat ggtaatttga ggtgtatcgg tttttggagt agttggtcca
     1261 aatgccgcta ttgaactcgg ccaatacatt ttttttatcc ctttttctct cgctacttcc
     1321 agggtattta taaggccatc catatttaat tgccaggctt tttgcggaaa tttttcaccg
     1381 gtagcagaaa gcatggcagc catattatat atctgcgtaa gcttatactt atcggcaagt
     1441 tcggcgatgc gattgcggtc caaaacattc aattcttcga atggtcccga ttcggctaat
     1501 tcgccaaccg gtatctttat atcagcagct acaacattgc tgttgccata ttggctgcgc
     1561 agtaacatgg ttaattcagc gccaatctgg ccgcttgctc caattaacag tatcgtttct
     1621 ccaggcatgt atgcagttta acagaacaaa tttagactta cacgtaatat ttacaaattt
     1681 attgcgctaa gatataatga tttaccttta cgtgcgttcg taacctgaaa tgaataagat
     1741 acttgtagtg gtcggaacca gacccaattt tattaaaata gtgcagttta aacattatgc
     1801 tgcgttgtat ggcttcgatt tgaagattgt acacacgggg cagcattatg atgaaaacct
     1861 gtctgatgtg ttctttcagc aattaggcat cacacccgat tactttctgg atacacgcac
     1921 cgctaatccc gacatgcagt taaatttgat taaagcaaga cttaaccatt tactcaataa
     1981 tacttacaag ccggatttaa tggtcgtggt tggtgatgta aactctaccc gcgccgctgc
     2041 agaaactgct caaattttaa acattcctat tgcacatgtt gaaagcggat tgcgcagctt
     2101 tgatcaaagt atgccggaag aaattaatcg tattgtaact gataaccttg ccgaccattt
     2161 ttttgtgact gaacaaagtg gtatcgataa tttattggaa gagggtaaaa aagcagctga
     2221 catctatttt gtcggtaata ccatgattga tgcattagta gcgtatgatc aggtgattgc
     2281 tgaaaatacc atcatgacca aacttgatgt tactgaacgg gcatttgctt tggttactat
     2341 tcaccgccca tctaatgtag ataataaatc ggcactggaa caaaacattc atttattaca
     2401 aaatatcgcc attaaatatc ctgtaatttt tccggtgcat ccacggacaa aaaataaatt
     2461 ggagcaattt caattattat cgctcttaac atcaaacccc aatatccgtc tttgtgaacc
     2521 actcgattat tttgcatttc aaaaattaat tgcttcctgc aaatttattg ttaccgatag
     2581 tggtggcatt caggaagaag ctacttttag gaaaaaacct tgtattacca ttcgcccgaa
     2641 tacggagc
//
LOCUS       DAORKW010000518         2621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2312_length_2621_cov_5.144973, whole genome shotgun sequence.
ACCESSION   DAORKW010000518 DAORKW010000000
VERSION     DAORKW010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2312_length_2621_cov_5.144973"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(418..648)
                     /locus_tag="PLB46_14895"
     CDS             complement(418..648)
                     /locus_tag="PLB46_14895"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77863.1"
                     /translation="MRSILHMDLDSFFVSVEVIKNPSLKGKPVIVGGTAERGIVASCS
                     YEARKFGIHSAMATTMAKRLCPDAIFFAWLLS"
     gene            complement(842..>2621)
                     /locus_tag="PLB46_14900"
     CDS             complement(842..>2621)
                     /locus_tag="PLB46_14900"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HRF77864.1"
                     /translation="NALLSSSKNKFVHKIALFLLYLMITVMTSAQIVTLSEQNKTPTY
                     QEVIDYFTTIAAENAYMELFEMGPTDIGKPLHLLVIDTDKDFNPEAARAKGKSILLIN
                     NGIHPGEPDGIDACIQLLDKLSKSDDITTTLKNLVICIIPVYNIDGALNRNSTTRVNQ
                     DGPESYGFRGNARNFDLNRDFIKSETLNAQSFAQIFRTWDPDVFVDTHVSNGADYQYV
                     MTLIPTQHNKLPLPLDNYLKQTMLPVLYQEMENVGFPMTPYVNEIKQIPDEGIAGFLD
                     LGRYSTGYTAMFNTLGFMPETHMLKPYPQRVASTLALLEIYIHYCATRNSELISIRNE
                     AKQYLKTATQFPINWQLNMDSFEQFMFKGYEAKYKPSEVSGLPRLYYDRSSPFEKNIP
                     FYDTYNATLTVTAPKSYIIPQAWKDVIHRLQINKVAMQQLAKDTVMEVEVYYIDKFEN
                     TPNAFEGHYLHSKITVFKQIQKIPYYAGDYIVHVNQDCNNYIVQTLEPQAPDSFFAWG
                     FFDAILMQKEWFSDYVFEDLAAEILAKNPSIKAALTAKQQSDPEFAKDAWAQLAFVFE
                     LSEHKEITHRRYPVGRVLNDLVIEQN"
BASE COUNT          819 a          457 c          484 g          861 t
ORIGIN      
        1 gcatatgctg ctgtaactgg agcagtgtat cttaaattat cagctaaagt tgtaacagac
       61 gagatatata cttttgcttc agtataccaa tttaatctaa ccagttgttt gcctataaaa
      121 acatcattag gttgtacagc aaagaatatt gtaaggaaat gaactacact agacccatat
      181 acatctgaag aaaaagctag actacaaagg tatatagaga aatgcaagga gagagtaaca
      241 tattttgata tgcagcaatg aactagctta gaaataattg ttaataagat aatggagtta
      301 catgagcagt gatacaagat tattgcagta gatagtttat catcaataga agatttttcc
      361 aaaacaggca ctctttcagc aataatatca gtaacccgtt tcgaataatc agcataatca
      421 tgataagagc catgcaaaaa aaatcgcgtc gggacataac cgttttgcca tggtggtcgc
      481 cattgcagaa tgtatcccga attttcgcgc ttcataacta caactcgcca caattcctct
      541 ttctgcagta ccacccacaa taaccggttt ccctttcagt gaaggatttt taatcacttc
      601 taccgaaaca aaaaacgagt cgaggtccat gtggaggatt gagcgcatga gtggaggtat
      661 tttttgattg agcaatcccg atagctatcg ggagaagcaa gattgatcaa aattgaaaaa
      721 tgtagagtgc ccttatatgt tgttttttat gttaaattaa agatgttttt ttaaaattaa
      781 agtttgtttg tataaactca aataagactg ttaaagccaa aaaattctgc ctcaatcttg
      841 ctcaattttg ttcaataacc aaatcattca acaccctccc caccggatac ctcctatggg
      901 ttatttcttt atgttctgat aattcgaaaa caaaagctaa ctgtgcccag gcatctttgg
      961 caaattcggg atcggattgt tgtttggctg ttaatgcggc tttaatagat ggatttttag
     1021 ctaatatttc tgctgctaaa tcttcaaaca cataatcgct aaaccattct ttttgcatta
     1081 aaatcgcatc aaaaaatccc catgcaaaaa atgaatccgg tgcctgtggt tccaaggttt
     1141 gaacaatata attattacaa tcctgattta cgtgaacgat ataatcacct gcgtaatagg
     1201 gtattttttg tatctgttta aacacggtaa ttttactatg caaataatgc ccttcaaaag
     1261 catttggtgt attttcaaat ttatcgatat aatacacttc aacttccata actgtgtctt
     1321 ttgccaattg ctgcatagct actttattaa tttgtaagcg atgaatgaca tctttccatg
     1381 cttgtggaat aatgtaggat tttggagctg tgactgtaag tgtagcattg taggtatcgt
     1441 aaaatggaat atttttttca aaaggcgagg agcgatcgta atataatctt ggcaaaccgc
     1501 ttacttcact aggtttgtat tttgcttcgt accctttaaa cataaattgt tcgaagctgt
     1561 ccatatttaa ttgccaattg attggaaatt gtgtggctgt tttcaggtac tgttttgctt
     1621 cgttgcgaat actaattaat tcgctattgc gtgttgcaca atagtgaata taaatttcca
     1681 atagtgccag ggtagaggct acacgttgtg ggtatggttt taacatatgt gtttcgggca
     1741 taaatccgag tgtattaaac atggccgtat aaccggtgga atatcggcct aagtctaaaa
     1801 atccggcaat gccttcatcg ggaatttgtt ttatttcatt cacatatggt gtcatgggaa
     1861 aaccaacatt ttccatttcc tgatataata ccggcagcat ggtttgtttc aaataattat
     1921 ccagcggcaa tggcaattta ttatgttggg taggaattaa tgtcatcaca tattgataat
     1981 ctgcaccatt actcacgtgt gtatcaacaa aaacatcggg atcccatgtt ctgaaaattt
     2041 gcgcaaaact ttgtgcattt aatgtttcgc ttttaataaa atcacgattt aaatcgaagt
     2101 tgcgggcatt ccctctgaag ccatatgatt ccggaccatc ctgattaaca cgagtggtgg
     2161 agttgcgatt taatgcacca tcgatattgt aaacagggat aatacaaatt accaggtttt
     2221 taagggtagt agtaatgtca tccgatttag ataatttatc caataattga atacaggcat
     2281 caataccatc cggttcgccg gggtgaatac cattattaat aagcaaaatt gattttcctt
     2341 ttgctctggc tgcttcggga ttaaaatctt tatccgtatc aattacaaga agatgtaatg
     2401 gtttaccaat atcagtcggt cccatctcaa ataactccat gtaagcattt tctgctgcaa
     2461 tggtggtgaa ataatcgatt acctcctgat aggttggcgt tttattttgt tcgctgagcg
     2521 ttactatttg tgctgatgtc ataacagtta tcattaaata gagtaaaaac aaggcaattt
     2581 tgtgcacgaa tttattttta gacgaagata ataatgcatt t
//
LOCUS       DAORKW010000519         2617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2315_length_2617_cov_4.198283, whole genome shotgun sequence.
ACCESSION   DAORKW010000519 DAORKW010000000
VERSION     DAORKW010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2315_length_2617_cov_4.198283"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PLB46_14905"
     CDS             complement(<1..613)
                     /locus_tag="PLB46_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRF77865.1"
                     /translation="MKWIPVCFLLMVVMMTSCRHESENADKTIFRLNLATPLTSLDPA
                     FASDQSNTWSVNQLFSGLVQLDSALNVKPCIAKSWEIADDRKTYTFHLRNDVYFHDDA
                     CFPNGKGRKLVAADVVYSFYRLIDPATAARGNWVFQNIVDSISPFYAINDSTVKIKLQ
                     QPFAPFLQRLSIQYCSIVPKEAIDTYGKDFRSHPVGTGPFKFLK"
     gene            complement(650..1132)
                     /locus_tag="PLB46_14910"
     CDS             complement(650..1132)
                     /locus_tag="PLB46_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77866.1"
                     /translation="MFQINGEDYSRKMNNYAIFADAPEIGNYIESISDKNKVVGAMTN
                     EPELLFYANRKSASGYLYMYPLFEYHPYFEQMTKEYMEQIVTQKPEIFIRTNIRNTGK
                     NKNNIDIVVQWWESYKVNYELLRVYVTTGPETPYQPLESLDNYTSSGNYVEIYRRISK
                     "
     gene            complement(1174..2250)
                     /locus_tag="PLB46_14915"
     CDS             complement(1174..2250)
                     /locus_tag="PLB46_14915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRF77867.1"
                     /translation="MQTSHGNQQLQQTNTISVMDMLMLVLAVAIAVYLRFNLLNIPFD
                     RDEGEYAYAGQQILQGNLPYDVIYNMKFPGVYYMYAAVFAVFGESIAAIRFTGICLHL
                     ATAFFLFGCVSFLFSKRTGIYAAATFLIISLSFPAESILSNAEVFVVFFGCAGLWMMT
                     QFLGKQKLIWLFFSGVCLCLSCLMKQPGAIYGVITLVVFLVYKPKTVSYYKLLVWIVG
                     GLLPAALLLGYLQYHELMSNFIFFCFIYGREYATMVNGIVGKYLFVTNYRDIFLNSWV
                     FVVISLLALVWSIVFYQQKKWWLLWLIVLLSALAVVPGLYFRKHYFIFLHPAVAIAVA
                     YFFLMEVKLSLQNSITILLPYCLQ"
     gene            complement(2382..>2617)
                     /locus_tag="PLB46_14920"
     CDS             complement(2382..>2617)
                     /locus_tag="PLB46_14920"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77868.1"
                     /translation="MLGLQHCIQYACVLNGSNSLDESDKKPSIICPECLAKLDIIFPT
                     FTEKYFPASLQFYQKYQFKEEQQFCERVINELK"
BASE COUNT          907 a          504 c          448 g          758 t
ORIGIN      
        1 attttaaaaa tttaaatggt cctgtaccta cagggtgact gcgaaaatct ttgccgtaag
       61 tatcaatggc ttccttaggt acaatagaac aatattgaat actcaatcgc tgcaaaaacg
      121 gagcaaaagg ttgttgcaat ttaattttta cggtgctgtc gttaatggca taaaaaggac
      181 tgatactatc cacaatattt tgaaaaaccc aattgcctct ggcggcagtt gccgggtcga
      241 ttaaccggta aaatgaatat acaacatcgg cagcaaccaa cttgcggcct ttgccattgg
      301 ggaaacaggc atcgtcgtga aaatatacat cgttgcgcaa atgaaaagtg taggtttttc
      361 tgtcatccgc aatttcccaa cttttggcaa tacatggttt cacatttaat gcgctgtcca
      421 attgcaccaa accactaaat aattgattta cactccaggt attcgactgg tcgctggcaa
      481 atgcagggtc tagtgacgtt aaaggtgttg ccagatttaa acgaaaaata gttttatcgg
      541 cattttcact ttcatggcga catgatgtca tcattaccac catcaacaaa aaacaaactg
      601 gtatccactt catttgcatt gctgtaaaat taaaccaaac aaaagggttt tacttggaaa
      661 tacgtcggta aatctccaca taattgcctg aagaagtata gttatcaagg ctttcgagtg
      721 gttgataagg tgtttcggga ccggtagtta catacacccg caacagctca taattgactt
      781 tgtagctctc ccaccattgc actacaatgt cgatattatt tttgtttttg ccggtgttac
      841 gaatattggt gcgaataaat atttcaggtt tttgtgtaac aatttgctcc atatattctt
      901 tcgtcatttg ttcaaaatag ggatgatatt caaacaatgg atacatatac aaatatcccg
      961 aagctgattt acgattggca taaaataaca attccggttc gttggtcatg gcacctacta
     1021 ctttattctt atcacttata gattcaatgt aattgccaat ttcgggagca tcggcaaata
     1081 tggcgtaatt gttcattttg cgactgtaat cttcaccgtt tatttggaac atatcataat
     1141 tacgctgggc gaaaataaaa aattgcaaac cggctattgt aaacaatacg ggagcaaaat
     1201 agttattgaa ttttgtagcg ataattttac ttccatcaaa aaaaaataag cgacggctat
     1261 agccacagca ggatgtaaaa aaataaaata atgtttgcgg aaatacaatc ccggcaccac
     1321 agccaaagca gataataaaa cgatgagcca caatagccac catttttttt gttggtagaa
     1381 aacaatactc caaaccaatg ccaacagact gattaccaca aaaacccaac tattcaaaaa
     1441 tatatcgcgg tagttggtta cgaataaata tttgcccaca atgccattta ccatagtggc
     1501 atattctctg ccataaataa aacagaaaaa tataaaattg gacatcagtt cgtgatattg
     1561 cagataaccc aacaacagag ctgcgggcag caatccacca actatccaaa ctaacaattt
     1621 ataatacgat accgttttcg gtttgtaaac taaaaataca acaagtgtta ttacaccata
     1681 aatagctccc ggttgtttca tcaaacagga aaggcataaa cacacacctg aaaaaaacaa
     1741 ccaaattaat ttttgtttgc cgagaaattg tgtcatcatc cacaatcccg cacaaccaaa
     1801 aaacaccaca aatacctctg cattagataa aatggattct gccggaaagg aaagtgaaat
     1861 aattaaaaat gttgccgctg catatatacc tgttcgtttg gaaaataaaa atgatacgca
     1921 gccaaataaa aaaaatgcag tggctaaatg caaacatatt cctgtaaaac gaatcgcagc
     1981 aatactttca ccaaatacgg caaaaaccgc agcatacata taatacaccc ctggaaattt
     2041 catgttataa ataacatcat aaggtaagtt gccttgtaaa atttgctgac cggcataagc
     2101 atattcgcct tcgtcgcggt cgaatggaat gttgagtaag ttgaacctta agtaaacggc
     2161 aatggcaaca gccaaaacaa gcatcaacat atccatgacc gatatggtat tagtttgttg
     2221 aagttgttgg ttaccgtggc tcgtttgcat ttgaggcttt agctgctaaa aataacaatt
     2281 aatttaatac ctgttcccta tttggttttg cacaccaatc aactcaacag cttattatgg
     2341 gctcgaaaat taattgagta aaagtaaaaa gttactgata tttatttcaa ttcattaata
     2401 acccgttcac aaaattgctg ttcttcttta aattgatatt tttgataaaa ttgtaaagaa
     2461 gccgggaaat atttttcagt aaatgtcgga aaaataatat ccagtttggc caaacattcc
     2521 gggcaaatga ttgatggttt tttatcggac tcatccaatg aattactgcc atttaaaaca
     2581 caagcatact gaatacaatg ttgtaatcct aacatgt
//
LOCUS       DAORKW010000520         2609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2321_length_2609_cov_6.315583, whole genome shotgun sequence.
ACCESSION   DAORKW010000520 DAORKW010000000
VERSION     DAORKW010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2321_length_2609_cov_6.315583"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1304)
                     /locus_tag="PLB46_14925"
     CDS             complement(171..1304)
                     /locus_tag="PLB46_14925"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRF77869.1"
                     /translation="MNKLWSNNNIIKSTLLILITLAVVFGVVWLLMQITTILFYFIIA
                     AIISIIGKPICDFIQRFKIGKFGVPDSLAALLTMTILFGVLFGVVKIFTPLVVQQTNN
                     ISNIDTERVAESLQSQIVALDSMIVHFQDPNAPSQSVEQIINDNLRNWINVTSITHIF
                     NNIMGGLGNILVALFSITFISFFFLRDDKLFYNIVMVLTPTKFETNARRIMHSSKKYL
                     TRYFVGLIIQSVVVFILIAVGLLFLGFGANSWLIALFAGIINIIPYIGPIIGMLFGML
                     IGITTNLSVDPNFDITPMLIQLFILFQGVQLIDNFVSQPIIYSNSIKAHPLEVFFVVL
                     VFGNLAGVPGMIAAMPVYAFFRIIAKEFFSEAKIVRKMTQGLE"
     gene            complement(1425..2399)
                     /gene="arsM"
                     /locus_tag="PLB46_14930"
     CDS             complement(1425..2399)
                     /gene="arsM"
                     /locus_tag="PLB46_14930"
                     /inference="COORDINATES: protein motif:HMM:NF038099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis arsenite
                     methyltransferase ArsM"
                     /protein_id="HRF77870.1"
                     /translation="MSTTYLETTRNVYKEAAENPQKGLCCTTTPVWQLPGLVIPQKML
                     EMNYGCGSTVNPRDLVNNPNILYVGVGGGMELLQFAYFSRKKGGVIGVDVVDEMLQAC
                     EENLIEAEKLNPWFKREFIDLRKGDAFNLPVEDNSIDIAAQNCLFNIFRDEDLKTALT
                     EMYRTLKKHGRLVMSDPTCEQTIPENLRDDEKLRALCLSGSLPLQEYINRITDIGFGT
                     IEVRARRPYRILDPVNYDTPELIFIESAEVCAIKDPMPADGPCVFTGKTAIYYGNEEY
                     FDDHKGHVLMPNQPLAVCDKTGGNLAKLGRNDIFISNSSWFYDGGGCC"
     gene            complement(2413..>2609)
                     /locus_tag="PLB46_14935"
     CDS             complement(2413..>2609)
                     /locus_tag="PLB46_14935"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRF77871.1"
                     /translation="WTASASGLPVSTTHVSVGGIFGIGVVNKKADYKMIGKIAGSWVL
                     TLPIAAILSGIIFFITSNII"
BASE COUNT          870 a          471 c          470 g          798 t
ORIGIN      
        1 acgaagtgca tgctgccacg aaaggaattc aaaactgcaa aaaaaaatgg caataaatca
       61 aaaaaataca ataagtgttt gacacaaata cataactgat ttggtaatca aagcttgatt
      121 atcagcgatt tactgaatcg taacgcataa aacatcagat ttgcctgtca ctattccaag
      181 ccttgtgtca tttttcgtac aattttagcc tcactaaaaa actctttggc tataatgcgg
      241 aaaaaggcgt aaacaggcat tgcagctatc attcccggaa caccagccag gttaccaaat
      301 actaatacta caaaaaacac ttccagcgga tgcgctttta tcgaattgga gtatatgatg
      361 ggctgtgata caaaattgtc tattaattga acaccctgaa ataaaataaa taattggata
      421 agcattggtg tgatgtcgaa attagggtca acacttaaat tagttgtaat accaattagc
      481 ataccgaaca gcataccaat tattggccct atgtatggga taatattgat aataccggca
      541 aataaggcaa ttaaccagct gtttgcacca aatcctaaaa ataaaagtcc tactgcaatt
      601 aaaataaaaa caacaacgga ttggataatt aatcctacaa agtaacgcgt caggtatttt
      661 ttgctggagt gcatgattcg cctggcatta gtttcaaatt ttgttggtgt taacaccatt
      721 acgatattgt aaaataattt atcatcgcgc aaaaagaaaa aggatataaa tgttattgaa
      781 aacagtgcca ccagtatatt gccaaggcca cccattatgt tattgaaaat atgcgtgatg
      841 gaagttacat taatccagtt gcgcaggttg tcgttaataa tttgttcaac agactgtgat
      901 ggagcattag ggtcttggaa atggacaatc atggagtcga gtgccacaat ttgtgattgt
      961 aaactttcgg caacgcgttc ggtatctatg ttagatatat tatttgtttg ctgaacaact
     1021 aatggtgtaa atattttaac aacaccaaat agcacaccga ataaaatggt cattgtaagc
     1081 aaagctgcca atgaatcggg tacaccgaat ttgccaattt taaaacgttg aataaaatcg
     1141 catattggtt tgccaataat agaaatgatt gctgcgatga taaaataaaa cagaatagtt
     1201 gttatttgca ttaatagcca cactacacca aataccacag caagtgtaat taggataagt
     1261 aatgtagatt taatgatatt attgttggac cagagtttgt tcatgttggt atggtattga
     1321 cgatgtaaat atacatagct gtatcaaagt ttggcaataa aaaaagccct tcacgtagaa
     1381 gggcttataa aaaaagatat ttctgttaca atgtgctaaa taaattagca acagccacca
     1441 ccatcataaa accagctgga gttggaaatg aagatgtcgt tgcgacctaa tttagccaga
     1501 ttacctccgg ttttatcgca aaccgcaagt ggttgattgg gcattaaaac atgtccttta
     1561 tggtcatcga agtattcttc attaccataa taaatagccg tttttccggt aaaaacgcat
     1621 gggccgtcgg caggcatcgg gtctttaatt gcgcatactt cagcgctttc aataaatatt
     1681 aattcaggtg tatcgtagtt aaccgggtct aagattcgat atgggcgacg tgcacgaact
     1741 tcaatggtgc caaaaccgat atcggtaatg cggtttatgt attcttgtaa tggtaaggag
     1801 ccacttaaac acaaggctct gagtttttcg tcgtcgcgta aattttccgg aatggtttgt
     1861 tcgcaggtag ggtcgctcat tactaaacga ccatgttttt ttaaggtgcg atacatttca
     1921 gttaatgctg ttttcaggtc ttcatcgcga aatatattaa acaaacaatt ttgagcggca
     1981 atatcaatac tattgtcttc aaccggtaaa ttaaatgcat caccttttct taaatcaata
     2041 aattcacgtt tgaaccaagg attgagtttt tcggcttcaa ttaaattttc ttcacaagcc
     2101 tgaagcattt catctacaac atcaacgcca attacaccac cttttttacg tgaaaaatag
     2161 gcgaattgta ataattccat tccgccacca acaccaacat ataaaatatt aggattattt
     2221 actaaatcac ggggatttac tgtagagcca cagccgtaat tcatttccaa cattttttgt
     2281 ggaattacta atcccggtaa ttgccataca ggtgttgtgg tgcaacataa acctttttgc
     2341 ggattttctg ctgcttcttt gtaaacgttt cttgtagttt ctaaataagt agttgacata
     2401 tgctttattg ttttaaataa tgtttgatgt aatgaaaaat ataattcctg ataatatcgc
     2461 agcaataggt aatgttaata cccatgaacc cgcaattttt ccaatcattt tataatctgc
     2521 ttttttgttt acgacaccaa taccaaaaat cccacctact gaaacatgcg tggtggatac
     2581 aggcaatcca cttgcactgg ctgtccaca
//
LOCUS       DAORKW010000521         2608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2323_length_2608_cov_3.469252, whole genome shotgun sequence.
ACCESSION   DAORKW010000521 DAORKW010000000
VERSION     DAORKW010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2323_length_2608_cov_3.469252"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLB46_14940"
     CDS             complement(<1..302)
                     /locus_tag="PLB46_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77872.1"
                     /translation="MKRTNWITSANLGIRGATLIGKFLLVYFLADQLTVEQNGVWGIF
                     TTSIALSLYVVGLDFYTYSSRRILDFKEEDRSPMLRDQLVFYFISYLVLFPILGLL"
     gene            complement(511..1689)
                     /locus_tag="PLB46_14945"
     CDS             complement(511..1689)
                     /locus_tag="PLB46_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF77873.1"
                     /translation="MKKYFPYITRPILVLSLVSLFTDMASEMLYPIMPVFLSSIGFSV
                     VLIGVLEGFAEMVAGLSKGYFGQLSDVRQKRLPFVQWGYFLSAISKPMLALFSTPIWI
                     FFSRTIDRTGKGLRSAPRDALLSNATTKENKAKVFGFHRSMDTFGAVLGPLIALWFLA
                     CYPGQYQTLFLIAFIPGLAAVLFTFFVKEKAGEPQQKKIHFFSFLSHVKNADGSYKKL
                     LWGLLIFALVNSSDVLLLLKMKSSGVSDVNIITIYIFYNLIYALSAFPAGIIADKWGI
                     KNTFLFGLILFIAVYIGFAFVNMEIGYWILFFIYGIYAACTEGIAKAWITNIVPAATA
                     GTAIGAYAGLASMAAFGASALAGVLWAVFGPEVAFGFTACVVTAVVFYLMRVPAATKA
                     "
     gene            complement(1699..>2608)
                     /locus_tag="PLB46_14950"
     CDS             complement(1699..>2608)
                     /locus_tag="PLB46_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77874.1"
                     /translation="DHFFLVKIPYDLPEDKYVKMIEFIPGNKKLAHHMNANLIEYQPG
                     KKTNVFGGQKIIATNLAESAESVQTAMDNKNDDGSYPTLVPLVCNYLPGVSPAKYPAG
                     IGGFRMSKQGAFFINDYHFGPSPKAAIDSTSKFNIFFADAPPQRPTYEILMGSLGVAP
                     VKPALVIPANEIKSFTIDYNVQETMSVLTVNPHMHLLGQSFIAYATTPRGDTIPMIRI
                     PKWEFRWQYFYTYKKVLVIPAGSVIHVEGVFDNTEKNINNPYFPPQVIIDRGDRFDSM
                     KTTNEMLQFIITYMAYQPGDENLSLE"
BASE COUNT          884 a          531 c          469 g          724 t
ORIGIN      
        1 aataaaagtc ccagaatggg aaacaatacc aaataactga taaaataaaa taccagctgg
       61 tcgcgcaaca ttgggctgcg gtcttcctct ttaaaatcta aaattcttct ggaagagtag
      121 gtataaaaat cgagccctac cacatacaag gataaagcaa ttgaagtggt gaaaatcccc
      181 caaacaccat tttgttctac cgtaagctga tcggctaaaa aataaaccag taaaaacttt
      241 ccgatcaggg tagctcctct aatacccaga ttagctgaag taatccaatt ggtccgtttc
      301 atcttttcaa taatattcat ggagtcaggc cgggcgattc aattggatga aatcaggtac
      361 atcgttaaac gggaaacaaa ggaggagttt gcggttggtc atacggtttt cctgaatcga
      421 aggcgaatat acggcgtatt tcgtgaattt gtgggagtag aaatgtggat gtaggtgttc
      481 atcggtattt acagcaaaag acgcaacatc tcaagcctta gttgcagccg gaacccgcat
      541 cagataaaac acgacagcag ttacaacaca tgctgtaaaa ccaaaagcaa cttcaggacc
      601 aaaaacagcc cataatacgc ctgctaatgc cgaagctcca aatgctgcca tacttgccaa
      661 accggcataa gcaccaattg ctgttcctgc ggttgctgca gggacaatat tggttatcca
      721 ggccttcgca attccttctg tgcaggcagc ataaatgcca taaataaaaa acaatatcca
      781 atagccaatt tccatgttta cgaaagcaaa cccgatgtaa acggcaataa ataaaatcaa
      841 tccaaacaag aaggtgtttt ttatacccca tttatctgca ataatgcctg cagggaaagc
      901 tgaaagcgca taaatcaaat tataaaaaat ataaatggta ataatattta catcgctaac
      961 gccgctagat ttcattttga gcaacaataa cacatcactg ctatttacta acgcaaaaat
     1021 gagtaaaccc cataacaatt ttttatagga accatccgca ttttttacat gtgaaagaaa
     1081 tgaaaaaaag tgaatctttt tttgttgcgg ttcgcctgct ttttctttta caaaaaaggt
     1141 aaataatact gcagctaaac ccgggataaa tgcaattaaa aataaagttt gatattgccc
     1201 cggataacaa gcgagaaacc acaatgcaat taatggaccc agcactgcac caaacgtatc
     1261 catagatcgg tggaacccaa aaacttttgc tttattttct ttggtggtag cattcgacaa
     1321 taacgcatcg cgaggcgcag accgtaagcc tttacctgtg cggtcaattg tgcgtgaaaa
     1381 aaatatccaa attggcgtgc tgaataatgc cagcatgggt tttgaaatgg cacttaaaaa
     1441 ataaccccat tgcacaaatg gcaaacgttt ttgacgaaca tcactaagct gtccaaaata
     1501 acctttgctt aaaccggcaa ccatttcagc aaacccttcc aatacaccaa ttaacaccac
     1561 agaaaaaccg attgaactca aaaaaaccgg cataattgga tataacattt cacttgccat
     1621 atcagtaaac aaactgacaa ggcttaatac taaaatcggg cgggtgatat agggaaaata
     1681 ttttttcact atgattattt attccagact taaattttca tcgccgggct ggtaggccat
     1741 gtaagttata ataaattgta acatttcatt tgtcgttttc atcgaatcaa atctgtcacc
     1801 tctgtctata attacctgtg gagggaaata agggttattg atattttttt ccgtattatc
     1861 aaaaacacct tccacatgaa taactgagcc agcaggaatt actaatactt ttttgtacgt
     1921 gtaaaaatat tgccaacgaa attcccattt aggaatacgt atcatcggaa tagtatcgcc
     1981 acgtggtgtt gtagcataag caataaacga ttgtcctaat aaatgcatgt gcggatttac
     2041 agtgagcaca ctcatggttt cctgcacatt atagtctata gtaaatgatt taatttcatt
     2101 agccggaatt accaatgcgg gttttacagg cgcaacacct aaggaaccca ttaatatttc
     2161 ataagtcggt cgttgcggag gcgcatctgc gaaaaaaata ttaaacttcg aagtgctgtc
     2221 tatagcagct ttcggtgatg gtccaaaatg ataatcatta ataaaaaacg caccctgttt
     2281 actcatacga aatccgccaa tgccggcagg atattttgca ggagatacac cgggtaaata
     2341 attacacacc aaaggcacta aagtaggata cgagccgtca tcatttttat tgtccattgc
     2401 agtttgcaca gattcagcgg actcagccaa atttgttgca attatttttt gccccccaaa
     2461 cacattagtt ttttttccgg gttgatattc aattaaattg gcattcatgt gatgcgccaa
     2521 ttttttattt cccggaataa attcaatcat ttttacatat ttatcttcag gcaaatcgta
     2581 aggtattttt accagaaaaa aatggtct
//
LOCUS       DAORKW010000522         2596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2338_length_2596_cov_4.993703, whole genome shotgun sequence.
ACCESSION   DAORKW010000522 DAORKW010000000
VERSION     DAORKW010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2338_length_2596_cov_4.993703"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLB46_14955"
     CDS             <1..918
                     /locus_tag="PLB46_14955"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRF77875.1"
                     /translation="NLSAMVLEGGAVEVDGAGTFLATRSSISGDNRNPTLTETDINNY
                     LQQYLGVSNIIWLDGLYGGALDITDTHIDGFAKFLDSTTLVTMNSADLSYWGISTSDI
                     TTLMTAENANGDTYNKIYLPLTNKNVKTTWGANVGFKSSYNNYYVGNNVVLATTFNDA
                     NDDDALAILSDLYPDKTIVGIDCRNLYFYGGMVHCVTQQQPIGLPLKSFVQFDNGDED
                     FPLTASISPNPASDITQITISGNITGNAVIEIYALNGALVYQTEVTTILPGNTSIAID
                     VSNFENGNYIGVVKNNNTRSSGFVIAVTH"
     gene            1033..2397
                     /gene="hemN"
                     /locus_tag="PLB46_14960"
     CDS             1033..2397
                     /gene="hemN"
                     /locus_tag="PLB46_14960"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRF77876.1"
                     /translation="MFSGLIQKYNVPGPRYTSYPTVPYWDEKGFSTEIWQQHLIKAFT
                     ESNAKEGISLYIHLPFCESLCTFCGCNKRITKQHNVEAPYIEAILKEWELYCNLFPEK
                     PIIKELHLGGGTPTFFSVEQLEKLINGIFLKAEKAEFPEYSFEGHPNNTTREHLQQLF
                     NLGFNRVSFGVQDYNEKVQIAIHRIQPFEHVERVTKWAREIGYQSIGHDLIFGLPFQT
                     LDDILFTINKTNELRPDRLAFYSYAHVPWIKGNGQRGFKDEDLPKDEEKRKLYETGKK
                     HLLDLGYLEIGMDHFALPSDNMFTAYNTGKLHRNFMGYTASKTQLMIGLGVSSIGDSW
                     YAFGQNEKEIEAYYEAINQNQLPIFKGHILNEEDLVIRKHILNIMCTFKTTWTDAMGY
                     FETLPQVIDDLAEMQADGLLQITAGKLTVTDAGKAFVRNICMAFDLQLKRKTPETKLF
                     SMTI"
BASE COUNT          879 a          432 c          474 g          811 t
ORIGIN      
        1 aatctttccg caatggtatt agaaggaggc gcagtagaag ttgatggtgc gggcacattt
       61 ttagctacgc gcagcagcat ttcaggtgat aatagaaatc ccacacttac agaaaccgat
      121 attaataatt atctgcaaca ataccttggc gtaagcaata ttatttggct cgatggttta
      181 tatggaggtg cgcttgatat cacagacaca catattgacg gatttgctaa atttttagac
      241 agcacaacat tggtaaccat gaattctgct gatttaagtt attggggaat ttccacttcc
      301 gatattacaa cattaatgac tgcagaaaat gcaaatggtg atacttataa taaaatctat
      361 ctgccattaa ccaataaaaa tgtaaaaaca acctggggtg ctaatgtagg atttaaatca
      421 tcctataata attattatgt tggaaataat gtagtattag ccaccacatt taatgatgca
      481 aatgatgatg atgccttagc tattttatcc gatttatatc ccgacaaaac aattgttgga
      541 attgattgtc gcaatttata tttttatggt ggtatggtgc attgtgtaac tcagcaacaa
      601 ccgataggtt tgccattaaa atcgtttgtt caatttgata atggagatga agattttcct
      661 ttaaccgctt caatttcgcc taaccctgca tcagatataa cgcaaattac catttcagga
      721 aatattacgg gtaatgcggt aattgaaatt tatgcactca acggggcctt ggtgtatcaa
      781 acagaagtaa caactatttt accgggaaat acttctatag ccatcgatgt aagcaatttc
      841 gaaaatggca attacattgg tgtagttaaa aacaataaca cacgcagcag tggttttgtc
      901 attgccgtaa cccattaatt acatatcaat tttaactatt ggggtgaaat tggtagcata
      961 tacctatttc atcccaaatt tgcaatgata actatcgcaa attcgtttag aatgttggcg
     1021 taaatttgcg ccatgttttc aggcctaatt caaaagtata atgttccggg accgcggtac
     1081 acgagttatc caactgtgcc atattgggac gaaaagggtt tttcaaccga aatttggcaa
     1141 caacatctta tcaaagcatt tactgaatct aatgctaaag agggaattag tttatacatt
     1201 catttgccct tttgcgaaag tctttgcaca ttttgcggtt gcaataaacg aatcaccaaa
     1261 caacataatg tagaagcacc ttatattgaa gccatactta aagagtggga attatattgt
     1321 aatctttttc cagaaaaacc tattatcaaa gaattgcatt taggtggtgg cacgccaact
     1381 ttttttagtg ttgaacaact ggaaaaatta attaatggaa tatttttaaa agccgaaaaa
     1441 gccgaatttc cggaatatag tttcgaagga catcctaata atactacacg tgaacatttg
     1501 caacaattat ttaatctggg atttaatcgc gtaagttttg gtgtgcagga ttacaatgaa
     1561 aaagtgcaaa ttgccattca tcgtattcag ccatttgaac atgttgaacg tgttaccaag
     1621 tgggctcgtg aaataggata tcaaagtatt gggcatgatt taatttttgg attgccgttt
     1681 caaacgctcg acgatatttt atttaccatt aataaaacaa acgaactccg ccctgacaga
     1741 cttgcttttt acagttatgc gcatgtgcct tggataaaag gaaatggaca acgcggattt
     1801 aaagatgaag atttaccaaa agatgaagaa aaaagaaaat tgtatgaaac cggtaaaaaa
     1861 catcttttag atttaggata tttggaaatt gggatggatc attttgcctt accttccgac
     1921 aatatgttta ctgcatataa taccggaaaa ttacatcgca attttatggg atacactgcc
     1981 tccaaaacgc aattaatgat tggattgggt gtttcatcaa ttggtgatag ctggtatgca
     2041 ttcggacaga atgaaaaaga aattgaagcg tattatgaag caataaatca aaatcaatta
     2101 cctattttca aaggtcacat tttaaatgag gaggacttgg taatcagaaa acatatcctt
     2161 aatatcatgt gtactttcaa aacaacatgg acagatgcca tgggatactt cgaaaccctg
     2221 ccacaggtta ttgacgattt ggctgaaatg caagccgatg gccttttgca gattacagca
     2281 ggtaagctaa cggtgacaga cgccggaaag gcatttgtcc gaaatatttg catggctttc
     2341 gatttgcagc ttaaacgcaa aactcctgag acaaagctgt tttccatgac tatatagcac
     2401 aaataaagtt aaaacaacaa tcttaatttg tacctttgct taatagttct ttgggcaaca
     2461 aatagtattt gcctattaat aatattaatt caacccacat tttaaaattt aaattgttgc
     2521 taattggggc ttaagctatt ttatttttat taattaacac taaaattaaa atgttatgaa
     2581 taaatcttat aacatc
//
LOCUS       DAORKW010000523         2594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2341_length_2594_cov_5.151635, whole genome shotgun sequence.
ACCESSION   DAORKW010000523 DAORKW010000000
VERSION     DAORKW010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2341_length_2594_cov_5.151635"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PLB46_14965"
     CDS             <1..419
                     /locus_tag="PLB46_14965"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRF77877.1"
                     /translation="AMMPFTEIAQKIGVSPGTIHVRMKKMDSIGLIKSSHLMVNPLKL
                     GYGITAFLGVYLDKSSMYDNVVKELEKIPEIVDCHYMTGTYSMFIKIICKDTMHLKQI
                     LHDSIQQIGGIDRTETFISLEESFSRPMKLELPKKN"
     gene            complement(1031..2281)
                     /locus_tag="PLB46_14970"
     CDS             complement(1031..2281)
                     /locus_tag="PLB46_14970"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HRF77878.1"
                     /translation="MLLNFFVTNTYTLSTFHTHYISTLEPAIARLNKQRKTATIAGFI
                     CRIFELYFVTMVVSLFLGFLDMFSSQDGVSESFFGAIRYFMYFVLTIVVVGLVNSQVK
                     KRLGEIDPKLALNNTRVGVLTLIFSAIAIGGGYLIGTLFLGSEFGLNFMLKWMLAIGS
                     VFVFLGPYFLLKKLEDRFTNHFKETLFSLSLPSIDKTIQYQASSGLTQQQFVNSKLFT
                     YQRIDTFKSRDYFANADKTFEGSYLDVMQIEQTQSNGKSETKYSQLFKGYLFVTDFNK
                     QTQGETYVFPDSARMLFGENTAERINELIHRPALKLAIMEDPEFEKLFAVYSTDAVEA
                     RFILSPKLIERITELKQHFYQDIHISIIQNKIYLAISSPDDLFAPGVFTDLHNEANIE
                     KFYERLRWLITFPEQFDLSTKVWG"
     gene            complement(2316..>2594)
                     /locus_tag="PLB46_14975"
     CDS             complement(2316..>2594)
                     /locus_tag="PLB46_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77879.1"
                     /translation="VFTWTDTLIEWRSYKGDSTDPLNETAYWSFDLDNPARVKNEGGN
                     SSEAIVIPGPGPNTNARINFWILPWIDTTPTDGLEQEIIVRRFEYEAM"
BASE COUNT          816 a          477 c          515 g          786 t
ORIGIN      
        1 atgctatgat gccatttacc gaaatcgcac aaaagatagg ggtttcaccg ggtaccatcc
       61 atgtccgaat gaagaaaatg gactccatag gtcttatcaa aagtagccac ctcatggtga
      121 acccactcaa gctcggatat gggattaccg cgtttttggg cgtgtatctc gataaaagtt
      181 ccatgtatga taatgtggtt aaagagctgg aaaaaattcc ggaaatagtg gattgtcact
      241 acatgaccgg aacctatagc atgttcatca aaatcatctg caaagacacc atgcatctca
      301 aacagattct tcacgatagc atccagcaaa tcggtggtat cgatagaacg gaaacattca
      361 tctcccttga ggaaagtttc agccgtccaa tgaagttgga gttgccgaaa aagaactgaa
      421 tagatgagta atgagggatg aataatgagt gattcaaact acgtttggca atccatgaat
      481 taatgtggat gtttgcaaac tttttagcaa ttgtttttta tcaccataaa ggctcgaaat
      541 agctcgaagt tacacgaagg attattagga tatttgctta gcgaaactat gaggaactta
      601 gtttcttcgt ggtaatttgt atcggtagat tgtaataatt ttatcacctc aaaggctcaa
      661 aggggctcga agttacacga aggattatta ggatatttgc tttgcgaaac tatgaggaac
      721 ttagtttctt cgtggtaatt tgtatcggtt gatagtaata atttttatca ccacgaagtc
      781 tcaaaggggc gctaagttac acgaaggatt attaggatat ttgcttagcg gaaatttgag
      841 gaccttagct tcttcgtggt aatttgtatc ggttgattgt aataattttt atcaccacga
      901 agtctcaatg gggctcgaag ttacacgaag ggttattagg atatttgctt tgtgaaactt
      961 tgaggacctt agcttcttcg tggtaatttt gttttggtag attgtaataa tttttatcac
     1021 cactcacaac tcatccccaa accttggtgg ataaatcaaa ttgttctggg aatgtaatca
     1081 gccatcgcaa acgctcataa aatttttcga tattggcttc gttgtgtaaa tcggtaaaca
     1141 cgcccggtgc aaataaatcg tcagggcttg aaatggcgag ataaatttta ttttggataa
     1201 tggaaatatg gatatcctga taaaaatgtt gtttcaattc ggtgatacgt tcaattaatt
     1261 tcggagataa aataaatcgg gcttctacag catcagttga ataaacggca aataattttt
     1321 caaactcagg atcttccata atagccagtt ttaaggctgg tcgatgtatg agctcgttga
     1381 tgcgctccgc agtattttct ccgaataaca ttctagcgct gtcggggaac acataggttt
     1441 caccctgggt ttgtttgttg aaatcggtga caaataaata acctttaaac aattgcgaat
     1501 attttgtttc cgattttccg ttactctgcg tttgttcaat ttgcatcaca tctaaataac
     1561 tgccttcaaa tgttttgtct gcattggcaa aataatcgcg acttttaaaa gtatcgattc
     1621 gctgataagt aaataatttg ctattgacaa attgttgttg tgtaagtcca ctacttgcct
     1681 gatattgaat tgttttatca attgatggca atgataagct gaataatgtt tctttaaaat
     1741 gattggtaaa tctgtcctct aattttttga gtaaaaaata ggggccaagg aatacaaata
     1801 ctgacccaat tgcgagcatc catttcaaca taaaattgag accaaattca gaccctaaaa
     1861 ataaagtccc aattaaataa cccccaccaa ttgcaatagc actaaaaatt aaagttaaaa
     1921 cgccaactct tgtattattt aaagccaatt tcgggtcaat ttcacctaaa cgttttttta
     1981 cttgtgaatt caccaatccc accaccacga ttgtcaaaac gaaatacata aaataccgaa
     2041 tcgcaccaaa aaaactttcg ctcacaccat cctgactgct aaacatatct aaaaacccca
     2101 aaaacaaact caccaccatg gtgacaaaat ataattcaaa aatgcggcaa ataaatccag
     2161 ctatggtagc tgttttgcgc tgtttattca atcgcgcaat ggcaggttct aaggtggaaa
     2221 tgtagtgggt gtggaaggtg gacaaggtat aggtatttgt tacaaaaaaa ttaagcaaca
     2281 aatataataa tgattgtcat caactatagc gacactcaca tcgcctcata ctcaaacctt
     2341 ctcacaataa tttcctgttc taaaccatcg gtaggcgtgg tgtcgatcca ggggagtatc
     2401 cagaagttga tgcgggcatt ggtgttggga ccagggccgg gaattacgat ggcttcagat
     2461 gaattgccac cttcgttttt tacacgagcc ggattgtcga ggtcgaaact ccagtaggct
     2521 gtttcattaa gcgggtcggt agaatcgcct ttatagctgc gccattcaat taaagtatca
     2581 gtccaggtaa atac
//
LOCUS       DAORKW010000524         2591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2344_length_2591_cov_4.444006, whole genome shotgun sequence.
ACCESSION   DAORKW010000524 DAORKW010000000
VERSION     DAORKW010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2344_length_2591_cov_4.444006"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLB46_14980"
     CDS             complement(<1..387)
                     /locus_tag="PLB46_14980"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HRF77880.1"
                     /translation="MKNLFTLAVLMSIVTLSFGQSDTTWKKGGGAILNFNQTSLTNWA
                     AGGESNLSSTILTNFYANYKFGKTTWDNTLNMNYGLITANNYSDIRKNEDRIELNSKY
                     GRYAFADDFYYSGMLNFLTQFAEGYDY"
     gene            complement(486..899)
                     /gene="mscL"
                     /locus_tag="PLB46_14985"
     CDS             complement(486..899)
                     /gene="mscL"
                     /locus_tag="PLB46_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRF77881.1"
                     /translation="MGMLKEFKDFAMKGNLIDLAVGFVMGAAFTAVTTSFINGIVMPL
                     VSLIAGKDFSAWKIVLRKAELDAAGVATKPEIAILYGTFISAAIYFIIVAFVMFIVIK
                     AINAMKKKEAEAPAAPPAPSAQEVLLAEIRDLLKK"
     gene            complement(995..1471)
                     /locus_tag="PLB46_14990"
     CDS             complement(995..1471)
                     /locus_tag="PLB46_14990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRF77882.1"
                     /translation="MENLFWKIKSFHQLNVFELYGILRLRQEVFALEQNCVYLDVDGK
                     DQFCDHIFGVDDAGKVIAYCRIVQPETGFSEVSIGRVATHPEHRNHGYGKLVMDKAIN
                     FIEDKYGPVSIKMEAQYYLEKFYSGYAFEPKSEVYLLDGIEHIEMVREKNAVHARV"
     gene            complement(1670..>2591)
                     /locus_tag="PLB46_14995"
     CDS             complement(1670..>2591)
                     /locus_tag="PLB46_14995"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRF77883.1"
                     /translation="DSIDVAYFFPEGFDSAVAKLQGTGFQYFYTSTNNEFEVAIIENI
                     NTVYKQALVKKKLAQIEVSETVLEPAKSISRNLASAREQLGSVIGGILPYFFIIFCMI
                     GCMYPAIDLAAGEKERGSLETLLVSSASRIEIYTGKLMTVALSGFLSAMASIVGLIIS
                     GQIIGSDTSSGDEMAQIAKVLSDILEPGSIIMMLVILLPLNIFFAAITLMLSIYARTF
                     KEAQSMITPLMILIVFPTIIGMLPGVTLDYTTALIPILNVSLGAKEIISGTIETGPLL
                     LTYAALFVYAAIALIISVRFFNNEKNILRG"
BASE COUNT          847 a          503 c          445 g          796 t
ORIGIN      
        1 ataatcgtaa ccttcggcaa attgcgttaa aaaattcaac attcctgaat aataaaagtc
       61 gtcagcaaaa gcatatcgac catatttact gtttaattcg atacggtctt cattttttct
      121 gatatcggaa taattatttg cagtaattaa accataattc atgttaaggg tattgtccca
      181 tgttgttttg ccaaatttgt aattggcata aaaattagtg aggatagttg acgacaagtt
      241 actttctccc ccggctgccc aattggtaag cgaagtttgg ttgaaattta aaatagcacc
      301 accacctttt ttccaagtgg tatcagattg gccaaatgat aacgtaacaa tgctcattag
      361 cacagcaagc gtaaataaat ttttcataaa tatgtttgta agtttgattt tatccccgat
      421 tggcaacaat cgagccaaat aaaaacacca atgccttcat tgtgaggaag gcattggttg
      481 gattattatt ttttcagtaa atcgcggatt tcagcgagta atacttcttg agcagaaggt
      541 gcaggtggag ctgcaggagc ttcagcttct tttttcttca tagcattgat agctttaatt
      601 actataaaca tcacaaaagc tacaatgatg aagtaaatag cagcggaaat aaaggtgcca
      661 tataaaattg caatttccgg ttttgttgca acaccggctg catccaattc agctttgcgc
      721 aatacaattt tccatgccga aaaatctttg ccggctatta aacttaccaa aggcataacg
      781 atgccattaa taaatgaagt tgtgactgct gtaaaggcag ctcccataac aaaaccgact
      841 gctaaatcga ttaagttgcc cttcatggca aaatctttga attcttttaa cattcccata
      901 atactgattt ttaggttaac aaatattgat ttattctgtc acaatagtaa caaaattttg
      961 caaaacaaat atcacatgtg gtgtgggtta cgggttaaac tcgggcatgt acagcgttct
     1021 tctcgcgtac catctcaata tgctcaattc cgtcgagtaa gtaaacctca cttttaggct
     1081 caaaagcata tccggaatag aatttctcca aataatactg ggcttccatt ttgatagaaa
     1141 ccggaccata tttatcctca ataaaattga ttgctttatc cattaccaat ttgccataac
     1201 cgtgattgcg gtgctcaggg tgagttgcta cccgtccgat ggaaacttca ctaaagccgg
     1261 tttccggttg aactatgcga caatatgcga ttactttacc tgcgtcatcc acaccgaaaa
     1321 tatggtcaca aaattggtct tttccatcaa catcaagata aacacagttt tgttccaatg
     1381 caaacacctc ctgccttaaa cgcaaaatgc catacaattc gaacacattt aactggtgaa
     1441 aactcttgat tttccaaaat aaattctcca tggatctaaa ttctaacgaa aattataaaa
     1501 acgatttaaa attaacaatg ctaacagtgt gtgggcctgc taattgtcaa cattcagtta
     1561 acaaaatttg ggattgcaat gttcaggtac aaaacgcaat tggcgaaaaa aaaataaccc
     1621 ggtaatggtg gattaccggg ttaagtgttc agtttaaagg aatttacttt tatcctctca
     1681 aaatgttttt ttcattattg aagaaacgca cactgataat caatgcaata gctgcataaa
     1741 caaataatgc cgcataggta agcaacagtg gtccggtttc aattgttcca gaaattattt
     1801 ctttagcacc gagcgaaacg ttcaaaatag gaatcagtgc agtggtgtaa tcaagtgtta
     1861 cccccggcaa cataccaata atggttggaa atacaattaa aatcattaaa ggcgtaatca
     1921 tactttgtgc ttccttaaat gttctggcat atatggataa cattaatgtg atagcagcaa
     1981 aaaatatgtt taatggtaat aagattacca gcatcataat aatggaacca ggttcgagaa
     2041 tatcggataa aactttagca atttgagcca tttcgtcgcc cgatgatgta tcgctcccga
     2101 ttatttgtcc cgaaattatc agaccaacaa tactggccat tgctgataaa aatccgctaa
     2161 gcgcaacagt catcagtttt ccggtataaa tttcaattct gcttgcggat gataccaata
     2221 atgtttccag cgatccacgt tctttttcac ctgctgctaa atcaattgcc ggatacatac
     2281 aaccaatcat acaaaaaatg ataaagaagt aaggtaaaat gccaccaatt acacttccca
     2341 attgttcgcg ggctgatgcc aggttgcgtg aaatactttt agcaggttcc aatacggttt
     2401 cacttacttc aatttgtgct aatttctttt taactaatgc ttgtttgtaa acggtattaa
     2461 tattttcaat aatagctact tcaaattcat tgttggttga cgtataaaaa tattgaaaac
     2521 ctgttccttg taattttgca acagccgaat caaatccttc cgggaaaaaa tacgcaacgt
     2581 cgatgctgtc g
//
LOCUS       DAORKW010000525         2584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2354_length_2584_cov_5.666667, whole genome shotgun sequence.
ACCESSION   DAORKW010000525 DAORKW010000000
VERSION     DAORKW010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2354_length_2584_cov_5.666667"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLB46_15000"
     CDS             <1..757
                     /locus_tag="PLB46_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77884.1"
                     /translation="FVQAIKNGVQEHWNLHTEISIKTQEEVMSLSKKGSKEYIVLSFA
                     NPYRQQSGSNLYKFSYSFDGDERVRDEMSAHMYGEKCLIMVPIEKVDAAEDFINNLYL
                     AISLPNEMIDNESIVFGIDFVNHVVKSKLADERYSHLDISKNADKLKTYQLAVNKNEW
                     DTELPEAEAEKIYGYDIEIMEAEAFNEVLNKGKAGIAYGVKMGPYIAIVLSDTKEVVY
                     VSGLGNQMITKMSELYNAKELEKLNKAIIDTRN"
     gene            922..1944
                     /locus_tag="PLB46_15005"
     CDS             922..1944
                     /locus_tag="PLB46_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77885.1"
                     /translation="MKFTKLILLTITLGIFTQIKAFGQFGSPLLTYKNDVPTQKECAE
                     ITKRKLIVLNQRWDLDKVDKFKRDKEFSLLEEYKDECAAYSNAYVQLIKEKWSLHDTF
                     EVYSFKQIKELYETGATDVVVLALVHITVEKGSDNKNEYKLDFAYSGNEKKMDENGFN
                     AQDFKSLILFPIEQLNELDYTFTKMYLGISLLRQVPTKEDIHYSIDMMNNIVQRKTLD
                     NKFTDNTIKQLSPKLKEYTLAISKNDWDEDISIKDAQVYYPYPIEIVEQEKFNTLITE
                     GATGYAYAFKLGTWMGIMFSDTKEIAYVSAPIDIPVKKATTLFSLRDLNKINEAVTNT
                     KIVFKE"
     gene            2118..>2584
                     /locus_tag="PLB46_15010"
     CDS             2118..>2584
                     /locus_tag="PLB46_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77886.1"
                     /translation="MKTKLLSYSGAASVFLLAGAAKADAQVIYHDIDPDVYMTGDPDD
                     YFLDVNMDGEHELKFGHTTNALGAFWGCYGNVTPNYSYCLINNYVYTYTYGWEFGAPL
                     DAGDPVPFGFNNPPVAINVQYLGAGWDTGVGLWVGEGDKYFGFQFYTPDGIDGP"
BASE COUNT          873 a          377 c          513 g          821 t
ORIGIN      
        1 atttgtacaa gctattaaaa acggtgttca ggaacattgg aatttgcata cagaaataag
       61 tataaaaacc caggaagaag taatgtcctt atccaaaaaa ggcagcaaag aatatatagt
      121 tttgagtttt gcaaatcctt acagacaaca gtcgggtagt aacttgtata aattcagcta
      181 ttcatttgac ggcgatgaaa gggtaagaga tgaaatgtct gcgcacatgt atggcgaaaa
      241 atgtttgatt atggtaccaa tcgaaaaagt ggatgctgct gaagatttta tcaataattt
      301 gtatttggct atttcattgc ccaatgagat gattgacaat gaaagtattg tgttcggtat
      361 tgattttgtt aatcacgtgg ttaaaagtaa acttgctgat gagaggtact ctcacctgga
      421 tatttcaaaa aatgcggata aattaaaaac atatcaatta gcggttaata aaaatgagtg
      481 ggatactgaa ttgcctgaag ctgaggcaga aaaaatttac ggttatgaca tcgaaataat
      541 ggaagctgaa gcatttaatg aagtgctaaa taaaggtaaa gcaggtatcg cgtatggtgt
      601 taaaatgggg ccttatattg caattgtgtt atctgatact aaagaagttg tttatgtttc
      661 aggattaggt aatcagatga ttactaaaat gtctgaactt tataatgcga aagaactgga
      721 aaagcttaat aaagcgatta ttgatacacg gaattagttt attgattaag aagttagatg
      781 agtgattaag ctttacggtc atttgcaaag tttaagcgtt gcataattta tactacaaaa
      841 ccagctaaca acaggttttg atagttgaca atttgcactt ttactttttc tgcatagatt
      901 tgccgttata acagcgcaac tatgaaattc actaaactga tattgctcac cataaccctg
      961 ggtattttca cgcaaataaa ggcttttggc caattcggaa gtcctttact tacatacaaa
     1021 aatgatgttc ctactcaaaa ggagtgtgcc gaaataacta aacgcaagct gattgtcctt
     1081 aatcaacgtt gggatttaga taaagtagat aagtttaaac gtgataagga attttcatta
     1141 ctcgaagaat ataaagatga atgtgcagca tacagcaatg cctatgtgca gctgataaaa
     1201 gaaaaatgga gtttacacga tacttttgaa gtatattcat ttaaacagat aaaagaattg
     1261 tatgaaaccg gtgctacaga tgttgtagta ttggctttgg tgcatattac cgttgaaaaa
     1321 ggaagcgata ataaaaatga atataaactt gattttgcat attctggcaa tgaaaagaaa
     1381 atggatgaga atggatttaa cgcacaggat tttaagtcgt tgattttatt tccaattgaa
     1441 caattaaatg aattggatta cacctttact aaaatgtatt tgggaataag tttgttacgc
     1501 caggttccaa caaaagaaga tattcattat agtatagata tgatgaataa tattgttcag
     1561 cgtaaaactt tggacaataa atttaccgat aatactatca aacaactttc tccaaaactc
     1621 aaagaatata cgcttgcgat ttcaaaaaat gattgggatg aagatattag tataaaagat
     1681 gctcaagtgt attatcccta tccgattgaa attgtcgaac aggaaaaatt caatacctta
     1741 attaccgaag gtgctacagg ctatgcttat gcatttaaac taggcacctg gatgggtata
     1801 atgttttctg acacaaaaga aattgcttac gtttcggcgc caatagatat tccggttaaa
     1861 aaagcaacta cgttattcag tttgcgcgat ttaaacaaaa taaacgaggc cgttaccaat
     1921 actaaaatag tgtttaagga ataaacctgc cttatctgtt taccgccatt tagcatgtaa
     1981 atttgagcgg taattatcaa gtgttaatta ttgcatatgt aagaaagggg ctctttttta
     2041 gggtacttat ataaattttt atgtattgtt tttgattttt caaaaattac ctataatttt
     2101 agttaaaatt aaattctatg aaaacaaaat tattatccta ttcaggtgct gcttcagtat
     2161 ttctgttagc aggtgctgcg aaggcagatg cgcaggttat ttaccacgac attgatccag
     2221 atgtttacat gaccggtgac ccggatgatt attttcttga cgttaacatg gatggagagc
     2281 atgaacttaa gttcggccac acaactaatg ccttaggtgc tttttggggc tgttacggca
     2341 acgttacacc taattattcg tactgtctta tcaataatta tgtttatacc tatacatatg
     2401 gctgggaatt tggagcacca cttgatgccg gtgaccctgt tccgtttggc ttcaacaatc
     2461 ctcctgtggc tattaatgtg caataccttg gtgccggatg ggatacaggt gttggtcttt
     2521 gggtaggaga gggcgataaa tatttcggat ttcaatttta tacccctgat ggtattgatg
     2581 gccc
//
LOCUS       DAORKW010000526         2577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2364_length_2577_cov_5.015067, whole genome shotgun sequence.
ACCESSION   DAORKW010000526 DAORKW010000000
VERSION     DAORKW010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2364_length_2577_cov_5.015067"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..>2577
                     /locus_tag="PLB46_15015"
     CDS             317..>2577
                     /locus_tag="PLB46_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77887.1"
                     /translation="MKNSSRKSMAGAISLLLIFLSSTSNLFAQDYPDEIIDTTGIVVE
                     TNTDGLDTVWYFGADATDTIDEEALRLGLNDSIININLGSTMKPVTKGLYGFNTAGIF
                     NRKSMPNDGSADDQWQWLSDLRPETLRFPGGAECRFMDVMKGPGYGYDIDKIARFYDV
                     TDSITDNPTLAEILADADDEEALSEWIDPGYIEDYLSYYNNWVEQNLLDSTHRFIDDF
                     INLVKKIETDNPGHTVKIIYCLNIFSNTATETKEIIQYLRDNPIHDLSVVFVEMGNEP
                     YFDYSRLMMGWYSFEDYWQYINGINNDSLDIYTVGSAVWNDHDFIGAFKNDALFTCKV
                     GIPAENLHDAIFALRDAGPAESGSRADDDWNLDLRTKYHIKEPITGLVNKYRHAFDAV
                     ILHPYYDGHNWDSIPLIQLDSTYACLNDDMNPNNDVWRFDTYDTRLEDAFDGIGINFR
                     TFIRTRYIESYNEHNSVLDFDLDSASKKELIVTEWNFKDQGDYREGQLNRIGVFSHGF
                     MHGYVTFEWFLKDIKLNYNANYRTGFHTYSTFHNYAGGGTNATIGPARKHELDFLGKD
                     TIPYSWNADANPNGRNYLMKRTTHFVYELLGEITKKDLKYLQGNFGIYIASVNVQPTV
                     FIDTAKEYLYIYFSNQRDTTQQYLLNTAGTTGIYPGGVLTIADTATIYCLKASKPYST
                     SGQGKNTLYKLNECYNDPYDVEFPIEITQVDTILNVPNCPGSGDPLKCLVVPAYSFGY
                     VKVPIEAYYPPERV"
BASE COUNT          879 a          411 c          478 g          809 t
ORIGIN      
        1 acattttgaa taaataattt tagattgata gtaaaattat aaaaatcagg gtttacttaa
       61 aatttataga gataaacata tgtgaataaa gactcttcac cagttgctga taccatttat
      121 attttagaat acttatgaaa ttgataataa ttttcagctc taattaacta ataatgaatt
      181 tgattaacgg ttttccgtga atctattgca caaaatgtaa tttaacaaaa cttcaataag
      241 ttaaacgtta gatttgttta gcataatact agaaaagtat tagttcttta actttcttaa
      301 aaccaaattt actctaatga aaaactcaag tcgaaaatca atggcaggag ccatttcgtt
      361 gttattaatt tttctttctt ctacaagcaa cctttttgca caggattacc ctgatgaaat
      421 tattgatact accggaatag tagtagagac gaatactgat ggattagata ctgtttggta
      481 tttcggagct gatgctactg atactataga cgaagaagct ttgcgacttg gtttaaatga
      541 ttcgataatc aatattaatc ttggttctac aatgaaacca gttacaaaag gcttatatgg
      601 gtttaatact gctggaattt ttaatagaaa atctatgcca aatgatggtt ctgccgatga
      661 ccaatggcaa tggctcagcg atctaaggcc tgagacattg cgcttccctg gaggcgctga
      721 atgcaggttt atggatgtta tgaagggacc aggttatggt tatgacattg ataaaattgc
      781 acgattttat gatgttacag atagcataac agataatcct accttggcag aaatacttgc
      841 tgatgcagat gatgaagaag cgctttccga atggattgac cctgggtata tagaggatta
      901 ccttagttat tataataatt gggttgaaca aaatttatta gatagcaccc atagattcat
      961 tgatgatttt atcaacttag taaaaaagat tgaaactgat aacccgggtc ataccgtaaa
     1021 aattatttac tgcttaaata tatttagtaa tacagctaca gaaaccaaag aaataattca
     1081 atatttacga gataacccga ttcatgacct ttccgttgtt tttgtagaaa tgggaaatga
     1141 accatatttc gattattcta gattaatgat gggctggtat tcctttgaag attattggca
     1201 atatattaat ggtataaata atgactcact agatatatat accgtaggga gcgcagtttg
     1261 gaacgaccat gattttatcg gcgcttttaa aaacgatgct ttattcacat gtaaagttgg
     1321 tataccagct gaaaatcttc atgatgcaat atttgccttg cgagatgctg gtcccgcaga
     1381 aagcggatca agggcagatg acgattggaa tttagaccta agaacgaaat accatattaa
     1441 agagcctatt actggtttgg tgaataaata cagacatgca tttgatgctg ttattttaca
     1501 cccctattat gatgggcaca attgggactc aatcccacta attcagcttg attcgacata
     1561 tgcttgccta aacgatgata tgaacccaaa taatgatgtg tggcgctttg atacttacga
     1621 tacaaggctt gaagatgcat ttgatggaat agggataaat tttagaacgt ttataagaac
     1681 acgatatata gagtcatata acgaacacaa ctcagttctt gatttcgacc ttgattctgc
     1741 ttcaaaaaaa gaattaatag ttacagaatg gaattttaaa gaccaaggag attaccgtga
     1801 aggacaatta aacagaatag gtgtattctc acatggcttt atgcacgggt atgttacttt
     1861 tgaatggttt ttaaaggaca taaagttaaa ctacaatgca aattacagaa ctggttttca
     1921 cacttattca acatttcaca attatgccgg tggtggcact aatgctacta ttggacctgc
     1981 tcgaaaacat gaacttgatt ttttaggcaa ggataccata ccatatagct ggaatgccga
     2041 tgcaaaccct aatggacgca actatttaat gaagcggaca actcattttg tatatgagtt
     2101 acttggagaa attacaaaaa aagatttgaa gtatctacaa ggtaacttcg gtatatatat
     2161 tgcaagtgtc aacgtacagc ctacagtttt tattgatacc gcaaaggagt acctttacat
     2221 ttatttttcc aaccaaaggg atacaacaca gcaatacttg ctaaatactg cgggaacgac
     2281 cggaatttat cctggcgggg tcctaacaat tgcagataca gcaactattt attgtttaaa
     2341 ggcatcaaaa ccatattcta cttcagggca aggtaaaaac acactttata aattaaacga
     2401 atgttataat gacccatacg atgttgaatt tccaatcgaa ataactcaag tagacactat
     2461 tttaaatgtt ccaaactgtc ctggaagtgg agatccgtta aaatgtttag tggtgcccgc
     2521 ttattcattt ggttacgtta aggtgccaat tgaggcgtat tacccgccgg aaagagt
//
LOCUS       DAORKW010000527         2566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2379_length_2566_cov_5.097172, whole genome shotgun sequence.
ACCESSION   DAORKW010000527 DAORKW010000000
VERSION     DAORKW010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2379_length_2566_cov_5.097172"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1148
                     /locus_tag="PLB46_15020"
     CDS             204..1148
                     /locus_tag="PLB46_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRF77888.1"
                     /translation="MRISGFTFIRNADKLDYPIKESIQSVLPLVDEFIIAMGDNDEGD
                     TTFELLQSINSPKIKIINTVWDTVAFPKAQVYAQQTDLARAHCTGDWLFYIQGDEVIH
                     EKDYATILSRCEELLPNTAIEGLLFKYFHFWGDYWHQNRSHSAYGQEIRIIRNHPDIH
                     SYGDAQGFRRIPNFDGKNFRLKEGVYKLNVARVDAGIYHYGWVRPPDFMMRKRKMSNT
                     LHHGQSTTTENFAATIFDYGPVGRKPIFKGTHPAIMQARIAQFDWGNMLNYSKHQKKI
                     NRPLQKHEKLKYRIWSWFEIYVFKKQIFTAAKYVVKEV"
     gene            complement(1502..2125)
                     /locus_tag="PLB46_15025"
     CDS             complement(1502..2125)
                     /locus_tag="PLB46_15025"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRF77889.1"
                     /translation="MTTKEKIFTKALELFNNEGVEYVGMRELAAHLNMRVSNITYYYP
                     TKDDLVFAISQGLNEANSKVVVEYDNITLLTFLNMLKQVFHNHVQYRCLLLSFVHLME
                     QNKIIKAQYKKTASKRNTAINANISHLIKNGFLQFKDKSDVEYLGSALSLIIRFWISE
                     AAVSQSRLSVENQIHHYLILTAKTIQPYTSKKGEKELLHFLDGLQAL"
     gene            2179..>2566
                     /locus_tag="PLB46_15030"
     CDS             2179..>2566
                     /locus_tag="PLB46_15030"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRF77890.1"
                     /translation="MQHTYPLTIQNCIGEQLTFTGIEKTANGDRVLVKGKCNPGAGPA
                     MHVHFKQEESLTVVSGQLGYQVLGQEPKLAGVGETVLFKRGTPHKFWAAGNQPMEISG
                     WIEPANSVVYFLSALYAAPNKSGNAQP"
BASE COUNT          789 a          441 c          537 g          799 t
ORIGIN      
        1 aaattggtgc tcagccaagt gataaatatt ttgcaccggc atctaatcgt caggtgttgt
       61 ttgaatatgt gcaatacctg atttcactgc ttaaaaagcc atcggagcaa tacaaagccg
      121 atgaccttcg tcgcctggaa gacatactgg caggggagta gcggtaattg ttatacattc
      181 atagtaaata ggtatttttg tttatgcgta tttccggatt cacatttatt cgcaatgccg
      241 ataagctcga ttatcccatc aaagaatcta ttcaaagtgt attgccttta gtggatgaat
      301 ttattattgc tatgggcgat aatgatgaag gtgatactac attcgaatta ttacaaagta
      361 tcaattcacc taaaattaaa attatcaata ctgtttggga tacggttgca tttccaaaag
      421 cacaggttta tgcgcagcaa accgatttgg caagagcgca ttgtacaggc gactggttat
      481 tttatattca gggagatgag gtaattcatg aaaaagatta tgcaaccatt ctaagtagat
      541 gtgaagaatt attaccgaat acagcaatag aaggattatt atttaagtat tttcattttt
      601 ggggtgatta ctggcatcag aatcgttcgc atagtgctta tggtcaggaa atacgtatta
      661 ttcgcaatca ccctgatata cattcgtatg gtgatgcgca ggggtttcgt cgtattccga
      721 attttgatgg aaaaaatttc cgactgaaag agggtgtata taaattaaat gtagcgcgtg
      781 tagatgccgg tatttatcat tatggttggg tgcgtccgcc ggattttatg atgcgcaaac
      841 gcaaaatgag taatacttta catcacggac aatcgacaac tacagaaaat tttgcggcaa
      901 ctatttttga ttacggacct gttggaagga aaccaatttt caaaggaacg catccggcta
      961 ttatgcaagc gagaatagcg caattcgatt ggggtaatat gcttaattat tccaaacatc
     1021 aaaaaaaaat aaatcgccca ttacaaaaac acgaaaaact caagtatcgt atttggagtt
     1081 ggtttgaaat ttatgtattt aaaaaacaaa ttttcacggc ggcgaagtat gtggtgaagg
     1141 aggtttagga tggaattcgg agatcaattg taagcacatg ggttaatgaa attacgagct
     1201 tcgaatgatt gattagcaaa ttagctgatt agcagattag ctgatggggg tttataggtg
     1261 gaatgacatt cttgctcaaa tttggctgtt tccgccccgg cagacgtttc gcgaattggt
     1321 acatcggtac attggtacat ttgcacattg attagtttct cgcaaagctg caaagagtta
     1381 agatttatat gatttgttat ttcaggcgcg ctaagtcaat aaaccagttt cgcgcggaaa
     1441 aagggaaaat gtcggagctg agaaccggct gtttccgcct cggcggaatc ttcgcattcc
     1501 atcacaaagc ctgcaatcca tccaaaaaat gcaataattc cttttcgcct tttttggaag
     1561 tatagggttg tatcgttttt gctgttaaaa ttaaatagtg atggatttgg ttttcaacac
     1621 ttaaccgact ttgggagact gctgcttctg atatccaaaa gcgaataatt aacgaaagtg
     1681 ctgaacccaa atattcaaca tctgatttat ccttgaattg gagaaaacca tttttaatga
     1741 gatgtgaaat attggcgtta attgctgtat ttcttttcga tgcggttttt ttgtattgcg
     1801 ctttaataat tttgttttgc tccatcaaat gtacaaagct gagcaataaa catctatatt
     1861 gtacatgatt atgaaacacc tgtttcagca tgttcagaaa tgttaataat gtaatgttat
     1921 cgtattcaac tactactttc gagttggctt catttaagcc ttgtgagata gcaaatacca
     1981 agtcatcctt cgttggataa taataggtga tattactcac acgcatgttc agatgggcgg
     2041 ctaattcgcg cataccgaca tattctaccc cttcattatt gaataattca agggctttag
     2101 taaaaatttt ttcttttgtg gtcataaaat ttggacattg tccaaattta agtatatttg
     2161 ttcaataaaa tcaattttat gcaacacact tatccactta ccatccaaaa ctgtataggc
     2221 gaacaactca cttttacagg catagaaaaa actgcaaatg gcgaccgcgt tttggtaaaa
     2281 ggcaaatgta atcccggtgc aggccctgcc atgcatgtgc attttaaaca agaagaatca
     2341 ttaacagtag tatccggaca attagggtat caggtgttgg ggcaggagcc caaattagca
     2401 ggagttgggg agactgtttt gtttaaacgc ggaacgccgc ataaattttg ggcagccggt
     2461 aatcaaccaa tggaaattag tggctggata gaacctgcaa attctgtggt ctatttttta
     2521 tctgcattat atgcggcccc aaataaatcc ggtaacgctc agcccc
//
LOCUS       DAORKW010000528         2562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2386_length_2562_cov_5.485042, whole genome shotgun sequence.
ACCESSION   DAORKW010000528 DAORKW010000000
VERSION     DAORKW010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2386_length_2562_cov_5.485042"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLB46_15035"
     CDS             complement(<1..377)
                     /locus_tag="PLB46_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HRF77891.1"
                     /translation="MTKTRLEAFSDGVIAIIITIMVLELKIPHEATWEALFELWPVTL
                     SYILSFINIGIYWGNHHHLLHTTRKLNSGIIWANLNLLFWLSLMPFATAWMGENHFAT
                     NTVAVYSAILLMSGVAYYILQRC"
     gene            complement(427..1293)
                     /locus_tag="PLB46_15040"
     CDS             complement(427..1293)
                     /locus_tag="PLB46_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HRF77892.1"
                     /translation="MLEVILSVESFLGVDAATAALIILNLIIIESLLSIDNAAVIATM
                     VMDLPKNQRGKALRYGILGAYVFRGICLIFAAYLMEIAWLKAAGGLYLLFLMIHHFYK
                     RNVPDKPEEVKKKNESLFYRYTVGLFGVFWSTVILVELMDLAFSIDNVFAAVALAENI
                     PNPQKLYIVCFGVFVGILAMRFVAQGFVRLLEKYPFMITSAFLVIGLLGAKLVLSFMC
                     EVVGRSAFCELVNGHNADMWVSIITIAIFVIPVITSKFFNFPKADKNPELKEIEELEK
                     LENQMDEDNVTT"
     gene            complement(1298..2332)
                     /gene="galE"
                     /locus_tag="PLB46_15045"
     CDS             complement(1298..2332)
                     /gene="galE"
                     /locus_tag="PLB46_15045"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRF77893.1"
                     /translation="MESTKTILITGGCGYIGSHTALDLLSKGYNVVSIDNLSRSKQFV
                     PERIKSISGQDFINYRIDLRDLPKLRTVLHDHTDIVGIIHFAAFKSVGESVQKPLIYF
                     DNNINSTINILRIAEEYAIPNFVFSSSCSVYGNTTELPVSENTPLSSPESPYGWTKLI
                     AEQMIASHAKTSNINFIALRYFNPVGAHESGKIGEIPFAEPENLVPNITQTAIGKKEQ
                     FVINGADYPTRDGSCIRDYVHVMDIAEAHTLALEFQIAGKQQNKFEVFNLGSGNGVSV
                     LELIHSFESVSGVPLNYVLGTRREGDVIAIYADNKKAAEQLNWFAKRNLDQMMQSAWN
                     WEQQLNNEQI"
BASE COUNT          866 a          494 c          389 g          813 t
ORIGIN      
        1 atacaacgtt gcaaaatata ataagcaact ccgctcatga gtaaaattgc actgtatact
       61 gcaactgtat ttgttgcgaa atgattttca cccatccatg cagttgcaaa tggcattagt
      121 gataaccaga ataacaaatt cagattcgcc cagatgatgc cactgtttag ttttcttgtt
      181 gtgtgtaata agtgatgatg attaccccaa taaataccaa tattgatgaa ggataaaatg
      241 taacttaaag ttacaggcca tagctcaaaa agtgcttccc atgttgcctc atgaggaatt
      301 tttaattcta aaaccatgat ggtgataata atagcaatta ccccatcgct aaaagcttcg
      361 agtcgagttt ttgtcatatt aaaagaaaga ttaagcctga aaaataattt tgcctaaaat
      421 accaaattag gttgtaacat tatcttcatc catttggttc tccaattttt ctaattcttc
      481 aatttctttt agctcggggt ttttatcggc ttttggaaag ttgaaaaatt ttgaagtaat
      541 tacgggaata acaaatatgg caatagtaat tattgatacc cacatatcag cattatggcc
      601 attaactaat tcacaaaaag cgcttcttcc aaccacttcg cacataaaag acaataccaa
      661 ttttgcacca agtaaaccta ttactaaaaa tgcagaagta atcatgaatg gatatttttc
      721 aagtaaacga acaaagccct gagcaacaaa acgcattgct aaaattccta caaatacccc
      781 aaaacaaact atatataatt tttgcggatt gggaatgttt tccgctaaag ctacagcagc
      841 aaatacattg tcaattgaaa atgcaaggtc cattaattcg acaagtatta cagttgacca
      901 aaacacgcca aacaatccaa cagtatagcg ataaaataaa ctttcatttt tctttttcac
      961 ctcttccggt ttatccggta catttcgttt ataaaagtga tgaatcatta aaaataataa
     1021 atataatccc ccggcagctt ttagccaggc tatctccatc aaatacgcag caaaaatcaa
     1081 acatatacct ctaaatacat atgcccctaa aataccgtat cgaagtgctt tacctcgttg
     1141 atttttgggt aagtccatta ccattgtagc aattactgca gcattatcaa ttgacaataa
     1201 actttcaatt atgattaaat tcaggatgat tagtgcagca gttgcagcat ccactcctaa
     1261 aaaagattca acagacaaaa ttacttccag cataagacta aatttgttcg ttatttaatt
     1321 gttgttccca attccaagcc gattgcatca tttggtcgag gttgcgtttt gcaaaccagt
     1381 ttaattgctc agccgctttt ttattatcag cgtaaatggc aataacatcc ccttctcttc
     1441 gagttcctaa aacataattt aacggcacgc cgctaactga ttcaaaagaa tgaattaatt
     1501 ctaatacaga aacaccatta ccacttccca aattaaatac ttcgaattta ttttgctgct
     1561 tacctgctat ttgaaattct aaagcaagtg tatgtgcttc agcaatatcc atcacatgca
     1621 cataatcgcg gatgcaacta ccatcgcggg ttgggtaatc ggctccgttt attacaaact
     1681 gctccttttt acctatggca gtttgagtaa tgtttggcac caggttttcc ggttcggcaa
     1741 atggaatttc tccaattttt ccgctttcgt gtgcacctac cggattaaaa taacgcaaag
     1801 caataaaatt gatattcgat gtttttgcat gcgatgcaat catctgttct gcgattagtt
     1861 tcgtccaacc gtaaggtgat tcgggggacg acaagggtgt attttcactt accggcaatt
     1921 cagttgtgtt gccatacaca ctgcacgatg atgaaaatac aaaattggga attgcatatt
     1981 cttctgctat tcgcaaaata ttaattgttg aattaatatt attatcaaaa taaattaatg
     2041 gcttctgcac cgactctcct actgatttaa atgcagcgaa atgtataata ccaacaatat
     2101 cggtatgatc atgcaatacc gtgcgcagtt ttggcaggtc gcgcaaatct attctataat
     2161 tgataaaatc ttggccggaa atggatttta tacgttcagg aacaaattgt tttgaacgcg
     2221 ataaattatc aatggaaaca acattataac ctttgctcaa taagtccaaa gctgtatgtg
     2281 aacctatgta accacaacca ccggtaatta aaatcgtttt tgttgattcc aaaatgaaaa
     2341 attaaaatga aatataaata ctaaatataa tgctaaaaaa aatttattta attaaacgca
     2401 ttaaatattg accataacca cttttcatta atggctgagc aattttttct aattcttcgg
     2461 cattaatgaa ttttttccta aatgcgattt cttcaataca accaatattt aatccctgac
     2521 gttgttctat caattgaata aaagtactgg ctgccattaa tg
//
LOCUS       DAORKW010000529         2557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2389_length_2557_cov_3.666667, whole genome shotgun sequence.
ACCESSION   DAORKW010000529 DAORKW010000000
VERSION     DAORKW010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2389_length_2557_cov_3.666667"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="PLB46_15050"
     CDS             <1..1275
                     /locus_tag="PLB46_15050"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF77894.1"
                     /translation="DALVSAVGPNGCYLGMGARDPNSRVSYGSFFTTGGTPEQAWSGT
                     GPYSGTGAVTEDWATQVAFKIPLIAAGECQCIAYAYILDEDDLEEALEATTAVGVAAD
                     GIDISDAGFTLICPGDSVELNIIDGDDYDWTWSPADGLSTTTGDSVTASPDVTTIYTA
                     VGVGICGTLEREVTVEVPEPPVADAGPDIDVCPGDTVHLLGSGGATYLWSPPVYLDDP
                     TLANPAVEGPLTNMFYMLTVFDANGCPATDNMGLYLRELPDVDAGEDQYMVDGGFAQL
                     NATGAVTYEWTPEDYLSNPVVPDPTAFPEDTIIYYVTGTDQFGCENTDSVTVFLLPQT
                     IIVSPTAFTPNGDGLNDTYKPVLVGLGQVTYFSIYNRWGELIYETSDPTQGWDGTRLS
                     LEQEVGNYVVLIRGIDAFGFSITKSSMVLLMR"
     gene            complement(1338..2270)
                     /locus_tag="PLB46_15055"
     CDS             complement(1338..2270)
                     /locus_tag="PLB46_15055"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HRF77895.1"
                     /translation="MILLQRFIAIGWILVSGQLMGQISADNYRIYSVKTGTEVTLSAI
                     VEDMANYDVVLYGEEHDDAVTHFIEAQLLTLMHQQYGEKIALSLEMFDRDVQPVMDEY
                     LQGSIREKHFTKDARVWSNYTDYKPMVEYAKTNGIPVICANAASRYTNLAGREGQKAL
                     MALPESSKAFMAPLPYDTASGKYYEKLVELTRHDPTTSTDSTGKQIAPVTMMSGFNLI
                     MGQSLWDATMAYSITQYLKSNKGKKVLQIYGRFHSDEGYAIVTQIKNYNKKIKPLVIS
                     AGPDLSFPVIEWKQFSYLGDYVIITDPAVPKSFE"
     gene            complement(2287..>2557)
                     /locus_tag="PLB46_15060"
     CDS             complement(2287..>2557)
                     /locus_tag="PLB46_15060"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRF77896.1"
                     /translation="FYQLKLKSTPFHDCSLQEVDFSQCDLTSVIFDNCDLSMAIFDNT
                     ILEKADLRNAYNYQINPEHNHIRKAKFSSNGLAGLLMQYDIDIYN"
BASE COUNT          733 a          468 c          506 g          850 t
ORIGIN      
        1 gacgccttgg tttctgctgt tggtccaaat ggttgttatt tgggtatggg agcaagggac
       61 ccaaattcac gagtgagtta tggttcgttt tttacaacag gtggaactcc ggaacaagca
      121 tggagtggaa caggcccata ttcaggaact ggagccgtta ctgaagactg ggcaacacaa
      181 gtggcattta aaattccttt aattgctgcc ggcgaatgcc agtgtatagc ttatgcctat
      241 atattagacg aagatgattt agaggaagcc cttgaagcaa caactgctgt aggggttgct
      301 gctgatggta ttgatatttc agatgccggt ttcaccttga tttgcccggg agattctgtt
      361 gaattaaata ttattgatgg tgatgattat gactggactt ggtcacctgc tgatggtcta
      421 agtacaacaa ctggcgactc tgttactgca tctcctgatg ttacaactat ttatacagct
      481 gtaggtgtag gtatttgtgg aacacttgaa cgtgaagtta ccgttgaagt tcctgaacca
      541 ccagttgcag atgccggtcc ggatatcgat gtttgtcctg gtgacactgt tcacttatta
      601 ggaagtggtg gtgcaaccta tttatggtca cctcctgttt accttgatga ccctacacta
      661 gcaaatcctg ctgttgaagg tccattaaca aatatgtttt atatgcttac cgtatttgat
      721 gctaatggct gccctgctac agataatatg ggtttatacc ttcgtgaatt accggatgtt
      781 gatgccggcg aagatcaata tatggtagat ggtggatttg cacaattaaa cgctaccgga
      841 gcggtaacct atgaatggac accggaagat tatttatcta atcctgtagt tccagatcct
      901 acagcattcc ctgaggacac cataatttat tacgtaacag gtactgacca atttggttgt
      961 gaaaacacag attctgttac ggtattttta ttacctcaaa ccattattgt atcaccaaca
     1021 gcatttacac ctaacggtga tggtttaaac gatacatata aaccggtatt agttggtctt
     1081 ggtcaggtta cttatttctc gatttataat cgctggggtg agttgattta tgaaacctca
     1141 gatccaaccc aagggtggga cggaacacgt ctttcacttg aacaagaagt tggtaactac
     1201 gtggtattaa ttagaggtat agatgcattt ggatttagta tcaccaaatc atcgatggta
     1261 ttgctaatgc gataaaaaaa taatgaaata aaaaaaaagc ctgattattt caggcttttt
     1321 ttatgcgcgt aaatctatta ttcaaatgac tttggaacag cagggtcggt gatgatgacg
     1381 tagtcgccta aataactaaa ttgtttccat tcaattaccg ggaaacttaa atcgggtcct
     1441 gcagaaataa ccaatggttt tattttttta ttataatttt ttatttgtgt tacaattgca
     1501 tatccttcat cgctatgaaa acgtccgtat atttgtaaaa cttttttgcc cttatttgat
     1561 tttaaatatt gtgttattga ataggccatt gtagcatccc aaagcgattg tcccattatt
     1621 aaattaaaac cactcatcat tgtaacaggt gcaatttgtt tgcctgtaga atcggttgat
     1681 gttgttgggt catgtcgtgt aagttcaacc aatttttcat aatatttacc tgaagcggta
     1741 tcgtatggca atggcgccat aaatgcttta gaactttcag gaagtgccat aagcgctttt
     1801 tgcccttctc tgcctgcaag gttggtatat ctgctagcag catttgcaca aataacagga
     1861 atgccgtttg ttttagcgta ttctaccatt ggtttgtagt cggtataatt gctccataca
     1921 cgcgcatctt tggtaaaatg tttttcacgt atactaccct gtaaatattc atccataaca
     1981 ggttgtacat cgcggtcaaa catctctagc gataaggcta ttttttctcc gtattgctga
     2041 tgcattaaag tgagtaactg tgcttctata aaatgtgtta ccgcatcatc atgttcttca
     2101 ccgtataata ctacatcata attagccata tcctccacaa ttgctgatag cgttacctct
     2161 gttcctgttt taacagaata tatacggtaa ttgtcagcac ttatttgccc cattaattga
     2221 ccgctaacca aaatccatcc aatggcaata aaacgttgta ataaaatcat agttaaaaat
     2281 ttaatattaa ttataaatgt ctatatcata ttgcattaat aatcctgcta atccgttgct
     2341 gctaaacttc gctttacgaa tgtggttgtg ctccgggttt atttgataat tgtatgcatt
     2401 acgtaaatct gctttttcta aaatagtatt atcgaaaata gccatggata aatcacaatt
     2461 atcaaatata acactggtta aatcacactg gctgaaatcg acttcctgca aggaacaatc
     2521 atgaaatggg gttgatttta attttaattg ataaaac
//
LOCUS       DAORKW010000530         2550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2403_length_2550_cov_4.812425, whole genome shotgun sequence.
ACCESSION   DAORKW010000530 DAORKW010000000
VERSION     DAORKW010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2403_length_2550_cov_4.812425"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLB46_15065"
     CDS             <1..488
                     /locus_tag="PLB46_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77897.1"
                     /translation="DERDTFKLRWDVSGKVGYMTRLNKDIWLGIPKDSILYDFTLKQG
                     DEFMLPKGAGLATVIKTDSIDLMEGGKRKRLTLKFENLAYQNKFGNLVWIEGIGSLNG
                     LFYFYDWINGTKTLINCYYDRDKKRYGESEECNETVISMEAVKGNVHKSVSVAFLSSI
                     F"
     gene            complement(692..1942)
                     /locus_tag="PLB46_15070"
     CDS             complement(692..1942)
                     /locus_tag="PLB46_15070"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HRF77898.1"
                     /translation="MATKKVKKTETPVFNFENFQKEAIAGLFGGKNLGGEDGVLAPLV
                     QHLLNAALKGEAKGHIKEDKSQGVKNRLNGTTNKRVKTEYGEVAITPPRDRNGTFEPQ
                     LLGKWDRKLGTGLNEQILYLYALGNSYTDIQHQLKMLYGVEMSTGTITAITDEVYSEL
                     SAWQNRRLESMYTVLFLDGIYFTSREENVSKKRVIYSVYGINCEGQRDVLGIYIRNVE
                     AASEWASILNDIKRRGVEDVLFVCVDGLAGFKEAIEQMFPMSLVQRCIVHMIRNCTKF
                     VSDKDIKKVCKDMKEIYQAADITQAEIALESFKEKWDVKYPDISKAWEKDWTELTNFM
                     GFSEHIRRIIYTTNAVEALHRQIRKVTKTKGSWTTDKSLIKQIYLILTYGKGGWKRDV
                     FGWKIIAKELQEKFGERYERHLMI"
BASE COUNT          728 a          533 c          402 g          887 t
ORIGIN      
        1 atgatgaaag agatactttt aaactgagat gggatgtatc aggaaaagta ggatacatga
       61 caaggttaaa caaagatatt tggctaggca tcccaaagga tagcatttta tatgatttta
      121 cgctaaagca aggagacgaa ttcatgctac ctaaaggagc aggtttggca acagtgatta
      181 agactgacag tattgactta atggagggtg gtaaaagaaa acgacttacc ctaaaatttg
      241 aaaatttagc ataccaaaat aaatttggta atctggtatg gatcgaaggc attggttcac
      301 taaatggttt gttctatttt tacgattgga tcaatggcac caagaccctt atcaattgtt
      361 attatgatag agataagaag agatatggcg aaagtgaaga atgcaatgaa actgtaatta
      421 gtatggaagc tgtaaaaggg aatgttcata aaagtgtgtc agttgctttt ttatcatcca
      481 ttttttaatc gtcactttct tttctcaagc tcggagaaca aaggttatga ggaaaccaaa
      541 aatcgtaggc ttgcccaatg tggaatttta attttgtcaa gggcggccgc taggccgttc
      601 attttaccct ttacaaaatt ataaaaattc cactaccttg ccgtcgattt ttggtttcct
      661 tagccggtgc tagttccttt gaacatcggt ttcatatcat caggtgcctt tcatatcttt
      721 caccaaattt ttcttggagc tcttttgcta ttattttcca gccaaacaca tctcgcttcc
      781 atccgccttt tccatatgtc agaataagat agatctgttt gattaaggat ttgtcagttg
      841 tccatgatcc ctttgtttta gtcactttcc ttatctgtct gtgcaatgct tctactgcat
      901 ttgttgtgta tatgatgcgt cgaatatgtt cgctaaatcc catgaaattc gtcagttcag
      961 tccagtcttt ttcccatgct tttgatatat ctggatattt gacatcccat ttttctttga
     1021 aactttccaa ggctatctct gcctgggtta tgtcagcggc ctggtatatt tctttcatat
     1081 ctttacatac ctttttaata tccttgtctg atacaaattt ggtgcagttc cttatcatat
     1141 gaactatgca tctttggacc aaactcatcg gaaacatctg ctctatggcc tctttaaatc
     1201 ctgccagccc gtcaacacat acaaatagca catcttctac accacgtcgc ttgatatcgt
     1261 tgaggatgct tgcccattca ctggctgcct ctacattgcg tatgtaaatg cccagtacat
     1321 ctctttgacc ctcacaattg ataccgtata ctgaataaat aaccctcttt tttgacacgt
     1381 tttcttcccg gcttgtaaag taaatgccgt ctaaaaacag aactgtgtac attgattcta
     1441 acctcctgtt ctgccacgca ctcagttcac tgtatacctc atctgttatg gcagtaatcg
     1501 ttccggtgct catctccact ccgtataaca tctttagttg atgctgtatg tcagtatagc
     1561 tattacctag ggcatataaa taaagaattt gttcattgag ccctgttccc aactttctgt
     1621 cccatttgcc aagcaactgc ggctcaaatg ttccatttcg atctctgggt ggcgttattg
     1681 ctacctctcc atactcggtc tttacccttt tattggtagt accatttaat ctgttcttaa
     1741 caccctgcga cttgtcttcc tttatgtgcc ccttagcttc tcccttgagt gcagcgttca
     1801 gcagatgctg taccaaaggg gctaacaccc catcttctcc acctaagttt ttgcccccaa
     1861 atagcccagc aattgcttct ttctgaaaat tctcaaaatt gaataccggt gtctctgtct
     1921 tttttacttt tttcgttgcc attgtcaaaa ttatttaatt tgttaaattt tgacacactt
     1981 ttttaaacag tctcaaattt agaaagctgt cgggatcaat tcctgacagc tttcaatgtt
     2041 tttacaacac cccccatcaa aaatatctta attctataaa accggcaatc aatagatgta
     2101 tcctacctat ttgcccagat aagaacctta gttataatgg tcaatagcat ccgaacactt
     2161 tactacatac ttcccaatat aattctgcta ttaagtcaaa gaaaagactc aatttaaatt
     2221 ttgtttcgct tttcatcatt tcatagaatt ttatatcgaa ttaatcactg agataaaatc
     2281 atgaacgcca tgttatattt acattcaatt tccttattgt tctaaatgtg tttgaatcgt
     2341 ttataatcga ctatatttga ataacaatct attaaaacag aaagcacaac taaaaaataa
     2401 gtataaataa aatgatccgg actactatca ataaaaattt atctgacaat ttcatagtat
     2461 agaaaattct tgcaaccaca tcattcaatg tcaaaatttt ttatcttact ctgctttaat
     2521 ttgtattctt tttacagctt cacccaatcc
//
LOCUS       DAORKW010000531         2547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2411_length_2547_cov_3.848315, whole genome shotgun sequence.
ACCESSION   DAORKW010000531 DAORKW010000000
VERSION     DAORKW010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2411_length_2547_cov_3.848315"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PLB46_15075"
     CDS             complement(<1..1190)
                     /locus_tag="PLB46_15075"
                     /inference="COORDINATES: protein motif:HMM:NF009667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HRF77899.1"
                     /translation="MNKEYQHTIKKSITVSGVGLHTGTQVDMTFHPAPANHGYKFRRT
                     DLDGQPVIEADADLVTDTARGTTITKNNASVSTIEHTLAALVGLGIDNVMIDLNGPET
                     PIMDGSSKEFIQALSSVGMETQDVEREYITISENITFKDALRKTEITVMPADDYQVTV
                     MIDFDSKVLGKQHATLNHISEFSGQIANSRTFCFLHELEMLYENNLIKGGDLNNAIVI
                     VDKEVTTDEMEKLKQMFKKDSVSVKKEGILNNLELHWNNEPARHKLLDVVGDLALAGK
                     PIKGKIIASRPGHPSNVEFAKKIKAYYKFRKNTVDVPVYDLKAEPLYDINKISKMLPH
                     RYPFLLVDKIIEMGENHIVGVKNITFNESFFQGHFPNNPVFPGVLQVEALAQTGGIFV
                     LSKVP"
     gene            complement(1209..2252)
                     /gene="lpxD"
                     /locus_tag="PLB46_15080"
     CDS             complement(1209..2252)
                     /gene="lpxD"
                     /locus_tag="PLB46_15080"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRF77900.1"
                     /translation="MEFTARELCDFLQGTLEGDPFVKVHKPAKIEEGVEGDVCFLANP
                     KYLAYAYTTKASVLIVNESQLFEQPVQATIIRVKDAYSSIAMVLDQYKIMNNASGKRT
                     VEQYTAISKLAKVGENVHIGAFTVVSDEAEIGNDTYIHPNVFIGLRVKIGKNTTIYPG
                     AVILDDCVIGNDVIIGSGTILGSDGFGFAPQPDGTYKKVAQTGNVVIEDNVEIGANTT
                     IDRATMGSTIIRKGAKLDNLIQIAHNVEIGENTVIAAQTGISGSTKVGKNCIVGGQVG
                     IVGHITIADGTRINAQSGVSKSIKGNNIAVTGSPAFEYTDSMRSQVIYRRLPELMEKL
                     AALEAKVSALENK"
     gene            complement(2257..>2547)
                     /locus_tag="PLB46_15085"
     CDS             complement(2257..>2547)
                     /locus_tag="PLB46_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77901.1"
                     /translation="KLFRIELESEPISAQKINKITSSVKSAYNIQDEFDLQYYVFNDV
                     TTNSAYNLEANNINIKLRSGNVVDVTNASDHLNLAALAGTVVKHYICYPKEN"
BASE COUNT          719 a          530 c          436 g          862 t
ORIGIN      
        1 ggcactttac ttaaaacaaa aattccacct gtttgtgcca atgcttccac ctgtaacacc
       61 cccggaaaaa caggattatt tggaaaatga ccttgaaaaa acgattcgtt gaaggtgata
      121 tttttaacac ctacaatatg attttcgccc atttcaatta ttttgtccac taacaaaaac
      181 ggataacgat gtggaagcat ttttgaaatc ttattgatgt cgtataacgg ttctgctttt
      241 aaatcataaa caggaacatc aacagtgttt tttctgaatt tatagtaagc tttaattttt
      301 ttagcaaatt caacatttga aggatggccc gggcgactcg caataatttt acctttaata
      361 ggttttcctg caagtgctaa gtcgccaaca acatctaaca atttatggcg tgcaggttca
      421 ttattccagt gtaattcaag attgtttaat atgccttctt tttttacgct gacagagtct
      481 tttttaaaca tctgtttcag cttttccatt tcatcggttg ttacttcttt atcaacaata
      541 acaatcgcat tatttaaatc accaccttta attaaattgt tttcatataa catttctaac
      601 tcgtgcaaaa aacaaaatgt tctgctattt gcaatttgtc ctgagaattc tgaaatatgg
      661 tttaaagtag catgttgttt tcccaacact tttgaatcaa aatcaatcat caccgtaacc
      721 tgataatcat cggcaggcat tacggtaatt tctgttttac gcaaagcatc tttaaacgtg
      781 atattttcgc tgatggtaat atattctctc tcaacatcct gagtttccat acccactgag
      841 gatagtgcct gaataaactc ttttgatgaa ccatccataa ttggggtttc aggaccatta
      901 aggtctatca tcacattatc gatacccaag ccaacaagag ccgcaagcgt atgttcaatg
      961 gttgaaacac tggcgttatt ttttgtaatg gtagtaccac gggctgtatc agttaccaaa
     1021 tcggcatctg cttcaataac aggctgacca tcgaggtctg ttcttctgaa tttataccca
     1081 tgatttgccg gagccggatg gaaggtcata tccacttgcg ttccggtatg taaaccaaca
     1141 ccagaaacgg ttatggattt ttttatggta tgctgatatt ctttgttcat tttctttgaa
     1201 tgtaaatctt acttattttc cagtgcacta acttttgctt ccaatgcagc taatttctcc
     1261 attagttctg gcaatctgcg gtaaattacc tgactgcgca tactatctgt atattcaaat
     1321 gccggtgaac cggtaactgc gatattattt ccttttatgc tttttgaaac tccactttgt
     1381 gcatttatgc gtgttccatc tgcaatggta atatgtccaa ctatgccaac ttgaccacct
     1441 acaatacaat ttttgcccac ttttgtgcta ccggaaatcc cggtttgggc agcaataaca
     1501 gtattttcac caatttcaac gttatgcgca atctgaataa ggttatccag ttttgcacct
     1561 ttcctgataa tggttgaacc catggtagcc ctgtcgatag tggtatttgc cccaatttca
     1621 acattatctt caatcactac attgccggtt tgtgccactt ttttataagt accgtcgggt
     1681 tgaggtgcaa agccaaagcc atcactgcca agaattgtgc ctgaaccgat aattacatca
     1741 ttacctatca cacaatcgtc taaaatcact gctccggggt aaatagttgt gtttttgccg
     1801 attttaaccc tcaatccaat aaaaacattt gggtggatat aggtgtcgtt accaatttca
     1861 gcctcatcac ttaccactgt aaatgcaccg atatgcacat tttcaccaac tttagctagt
     1921 ttcgagattg cagtatactg ttcaacggtt cgtttgccgg aggcgttgtt cattatttta
     1981 tactggtcta aaaccattgc tatacttgaa taagcatctt tcactctgat aatggtagct
     2041 tgaacaggtt gttcaaaaag ctgactttca ttcacaatca aaaccgatgc ttttgtagta
     2101 taagcgtagg ctaaatattt gggattggcg agaaaacaaa catctccttc aacaccttct
     2161 tcaatcttgg caggtttatg cacctttaca aacgggtctc cttcaagtgt tccttgtaaa
     2221 aagtcgcata gctcgcgtgc agtaaactcc attcggtcaa ttttctttcg ggtagcaaat
     2281 ataatgcttt acaacggtac ccgccaaagc tgcaaggttg agatggtcgc ttgcgttagt
     2341 cacatccaca acattaccgg agcgaagttt gatattaata ttattcgctt caagattata
     2401 ggcactattg gtggtaacat cgttaaatac atagtattgt aaatcgaatt catcctgaat
     2461 attataagcc gatttgacag aactagtgat tttattgatt ttctgagcgc tgattggttc
     2521 actttccagc tcaattcgga acaattt
//
LOCUS       DAORKW010000532         2544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2413_length_2544_cov_4.069506, whole genome shotgun sequence.
ACCESSION   DAORKW010000532 DAORKW010000000
VERSION     DAORKW010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2413_length_2544_cov_4.069506"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLB46_15090"
     CDS             complement(<1..222)
                     /locus_tag="PLB46_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77902.1"
                     /translation="MNNKYLLPVAFAAVLAFGVIIGVKISPFTAGRPSYASQNSSNQR
                     INDILNFISQRYVDSLNVDSLMDTFLSNYL"
     gene            415..1347
                     /locus_tag="PLB46_15095"
     CDS             415..1347
                     /locus_tag="PLB46_15095"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HRF77903.1"
                     /translation="MNEFIITFRESLEAALIVGIIYAVLDKNNLHHLKKSLWTSVIAA
                     IIASGILGVLLYNVLENAPKGTQELLEGVFMYITAALLFYVIFWMSKTLSNREVITNQ
                     TKDAAAKNGMWGIFFLVFFAIVREGFETALFLVASTSIDNNFSYGGFFIGILIAVLIG
                     YIIVIQGKKIQLRPFFKYTSLLLVFFAAGMVAYGTHEIHEYIEEREHAEAAAVVETTL
                     TDAAAAPVVHAEEEEYVYNIFKPKSAEEKPESFWYVEDKESGDYIHLLNDKGLIGSFF
                     KGLFGYNSNPILIEVILWFVALFFGLFIWRKAYK"
     gene            complement(1421..2032)
                     /locus_tag="PLB46_15100"
     CDS             complement(1421..2032)
                     /locus_tag="PLB46_15100"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRF77904.1"
                     /translation="MRITILGAGNIGLAAGTKWKAKGHDITFGVRDPQSQKAWNAINI
                     GAKVTTFQESMTDAEVVLVAIPFGVADEVLKSIDVEWDGKIIIDATNPISASTEPFES
                     AAEAISAWTGSADVVKAFNTTGVGNMTNPEYNGKAIETFICGDEVASKQTVSTLAQDL
                     GFRVVDVGGLENAVMLENLAKLWVTMAYKLGKGPNFAFTLVER"
     gene            complement(2076..2489)
                     /locus_tag="PLB46_15105"
     CDS             complement(2076..2489)
                     /locus_tag="PLB46_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HRF77905.1"
                     /translation="MKRNATAVWNGSGKEGSGHLSTQSVILNQNQYSWSTRFADGIGT
                     NPEELVAAAHAGCFSMKLSFVLGTKDLTPEAINTTCTITLDNGAITNAHLEVTAKVPG
                     ATAESFAEAAEEAKLNCPISKLLNTEITMTASLLA"
BASE COUNT          769 a          456 c          486 g          833 t
ORIGIN      
        1 gaggtagtta gagaggaagg tatccattaa cgaatccaca ttaagggagt ctacgtatcg
       61 ctgactgata aaattgagga tatcgttgat gcgctggttg gaggagttct gactggcata
      121 agatggtctg ccggccgtaa aaggggatat tttaacccct ataatcaccc caaacgctaa
      181 tacggctgca aaagcaaccg gcaataaata tttgttattc acctgaaaag tttaaagttt
      241 gcacaaaata actacatcta aaccatacct ccctatatcc tgacaacttc ttttgctcat
      301 acctaattta gggtatattg gatggaacag actttaacgc tttttccaac aaaattagtg
      361 cagtatgttt gcacctgttt agaattgttc taaataaata aagtcaacag ccatatgaat
      421 gaatttatta tcaccttccg tgaaagtctg gaagcggcgt taattgtagg tattatatat
      481 gcggttttag ataaaaacaa cctgcatcac ctaaagaaat cgctttggac gagtgtaata
      541 gccgcaataa ttgcttcagg tattttgggg gtattacttt acaatgtgct tgaaaatgca
      601 cctaaaggca ctcaggaatt attagaaggt gtgtttatgt atatcactgc tgcattatta
      661 ttttatgtga tattctggat gagtaaaacg ttatccaatc gtgaggttat tacaaatcaa
      721 actaaagatg ctgccgcgaa aaatgggatg tggggcatat ttttcctggt attttttgcc
      781 attgtcagag aaggattcga gacggcatta tttttagtag ccagtacaag catcgacaat
      841 aatttttctt acggcggatt tttcataggg attttaattg cagtattaat tggttatatc
      901 atcgtaattc aaggtaaaaa aattcaatta cgtcctttct ttaaatatac atccttgttg
      961 ctggtatttt ttgctgcagg tatggttgct tatggtacgc acgaaattca tgaatatata
     1021 gaagaacgcg aacatgctga agcagcagct gtggttgaaa ctacgcttac tgatgcggct
     1081 gcggcgcctg tagtgcatgc tgaggaagaa gaatatgttt acaacatttt taagccaaaa
     1141 tcagcggaag aaaaaccgga atcattttgg tatgttgagg ataaagaatc cggtgattat
     1201 attcacttgc ttaatgataa aggtttaatt ggttcttttt tcaaaggctt atttggttat
     1261 aacagtaatc cgattttaat agaagttatt ttatggtttg ttgcattgtt tttcggatta
     1321 tttatctgga gaaaagcata taaataaaat tttcgtacaa ataaaaagcg ctgtaattaa
     1381 ttcaactaca gcgctttttt tattaataag ttatttgcta ttaacgttct accaatgtaa
     1441 acgcgaaatt aggtccttta ccaagtttat aggccattgt aacccataat tttgctaagt
     1501 tctcaagcat tactgcattt tctaatccac caacatcaac aacacgaaaa cctaaatcct
     1561 gcgcaagtgt tgaaacagtt tgtttagatg caacttcatc tccgcaaata aatgtttcaa
     1621 tggctttgcc gttgtattca gggttagtca tattgcctac accggttgta ttaaatgctt
     1681 taaccacatc agcacttccc gtccatgcac taattgcttc ggcggctgat tcaaaaggtt
     1741 cggtacttgc agaaatagga ttggtagcat caattataat tttaccatcc cattcaacat
     1801 caatactttt taatacttca tcggcaacgc caaaaggaat agctactaac acaacttccg
     1861 catcggtcat tgattcctga aatgtggtaa cttttgcgcc gatattaatt gcgttccatg
     1921 ctttttgact ttgaggatca cgcacgccga acgtaatatc gtgacctttt gctttccatt
     1981 tagttcctgc agctaagcca atattaccgg ctcctaaaat tgttattcgc ataaattggg
     2041 tttgggatta ttgaataata tcaatagtat aaaaattatg ccaggagtga agcggtcatc
     2101 gtaatttcag tgttcagcaa cttagaaata gggcaattta atttagcctc ttcagcagct
     2161 tcagcaaaac tttcagcagt tgcaccaggt actttagctg ttacttctaa atgtgcattg
     2221 gtaatagcac cattgtccaa tgtaattgta caagtggtat ttattgcttc cggtgtaagg
     2281 tctttagtgc ctaatacaaa actgagtttc atactaaaac aacctgcatg cgcagcggca
     2341 acaagttctt cagggttggt acctatacca tcagcaaaac gagtgctcca tgaatattgg
     2401 ttttggttta atattactga ttgtgtagaa agatgacctg agccttcttt acctgagccg
     2461 ttccatacgg cagtagcgtt acgtttcatt ttttcttcga attttaattg ttatatgcac
     2521 cacaaaggta gtgcatataa caat
//
LOCUS       DAORKW010000533         2525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2444_length_2525_cov_4.864777, whole genome shotgun sequence.
ACCESSION   DAORKW010000533 DAORKW010000000
VERSION     DAORKW010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2444_length_2525_cov_4.864777"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLB46_15110"
     CDS             complement(<1..554)
                     /locus_tag="PLB46_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77906.1"
                     /translation="MKNYFTLALLLLLTLSVQAIDSKKIWYKNGQLKAEVGFENGLFN
                     GDFSSWYSNGQKKAEGKFLNNQRIGKWQLWDSLGTLRVVREYTDGFTFTTQLLKAVDG
                     AELPLSVYPKYPTATNNLGEPDYPALEKHQILVFKDLWRMIDNTPENALLFQDNTMRQ
                     VISNYLDIEGNAVFDDYKFNHLLN"
     gene            complement(625..1602)
                     /locus_tag="PLB46_15115"
     CDS             complement(625..1602)
                     /locus_tag="PLB46_15115"
                     /inference="COORDINATES: protein motif:HMM:NF021537.2"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HRF77907.1"
                     /translation="MKTCVVLLHVIFLPVFLQGQTRPEISTVDITYFWSAFDQLAGAK
                     NHADSVEIIQKNYIDVCSDVYSKYIHRSNISAEEYVTLFTIYPNYWRAIRPLTEQITN
                     RRVELEITLQKLAATIPGFKIPDIYFGIGCLRQGGTIKGNTIIIGAELAAADTLIDKS
                     ELSTWLKSVVGKTGDIVAMTAHESIHVQQTWFPMGEFFSLAKHRQLQLLNMAILEGSA
                     DYITASVLGLNINAGIREYGLQHECALYENFEASIANDPFDYSKWLYNGNSARDMPAD
                     MGYFIGYQITQAYFNSEADKNKAYKTIFKRGKYKKVFEESNYKHIVCGS"
     gene            complement(1652..2002)
                     /locus_tag="PLB46_15120"
     CDS             complement(1652..2002)
                     /locus_tag="PLB46_15120"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HRF77908.1"
                     /translation="MKSIITIIAIIAFGHIATAQTGSVATAVIASSIVCDHCLECGTC
                     GESIDDTLLGLNGVKNIAIDVKANTITVYYKPEKIALEEIKKAIVAIGYDADEMKADH
                     QAYDKLDACCKAPE"
     gene            2151..>2525
                     /locus_tag="PLB46_15125"
     CDS             2151..>2525
                     /locus_tag="PLB46_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77909.1"
                     /translation="MPYEKLIQSYVDNQLGIDVNFISPELALSLNNRLVDVFNTHKMV
                     QAGTGNKAAVHFDQLLRGDTIFWLDRAHENASENAFLDIIDAFVLHLNATCYAGITGY
                     EFHFARYEEGSFYTKHFDRFEQN"
BASE COUNT          784 a          514 c          394 g          833 t
ORIGIN      
        1 atgttcaaca agtgattaaa tttataatca tcaaacaccg cattcccttc aatatccaaa
       61 taattactta tcacctgacg catagtatta tcctgaaaca ataaagcatt ttcaggtgta
      121 ttgtctatca tgcgccataa atctttaaac accaaaattt gatgtttttc taaagcagga
      181 taatccggct cgcccaaatt atttgttgct gttggatatt taggatatac cgataaaggt
      241 aattccgctc catcaactgc ttttaatagc tgcgtcgtaa atgtaaatcc atcggtatat
      301 tcacgaacta cccgaagggt ccctaacgaa tcccataatt gccatttacc aatacgttga
      361 ttattgagga atttcccttc agcctttttt tggccgttac tgtaccaaga gctaaaatcg
      421 ccattaaaca aaccattttc aaagccaacc tcagctttta gctgaccgtt tttataccat
      481 attttttttg aatcaattgc ttgaactgag agtgttagca gcaataacaa ggctagggta
      541 aaataatttt tcatgtaagt agggtttatt caaatctttt gataaacgca tggccaataa
      601 aaaatatttt aattcagcat gaaattatga accgcaaact atatgtttat aattgctttc
      661 ctcaaaaacc ttcttatatt tcccacgctt aaaaatggtt ttatatgctt tgtttttatc
      721 tgcttcgcta ttaaaataag cttgcgtaat ttgatacccg ataaaatacc ccatatctgc
      781 aggcatgtcc cgagctgagt tgccattgta taaccatttc gaataatcaa atgggtcatt
      841 tgcaattgat gcttcgaaat tttcgtatag tgcacattca tgttgaaggc catattcacg
      901 tattccggca ttaatattta atcccaatac acttgctgta atataatcgg cactaccttc
      961 taaaatagcc atgttcaata attgcaactg ccggtgttta gccaacgaaa aaaattcgcc
     1021 catagggaac caggtttgtt gtacatgaat ggattcatgt gcagtcatgg ctacaatatc
     1081 acccgtttta ccaaccaccg attttaacca cgttgaaagt tcggatttat caattaatgt
     1141 atcagcggct gctaattcag caccaataat tatcgtattt ccctttatcg ttcctccctg
     1201 ccgtaaacaa ccaatgccga aataaatatc cggtatttta aatccgggaa tagttgcagc
     1261 taatttttgc agtgttattt cgagttctac tctcctattg gttatttgtt cagtaagcgg
     1321 acgaatagct cgccaataat tggggtaaat agtaaataat gttacatact cctctgcgga
     1381 aatattactt cggtgtatat atttactata tacatcacta caaacatcaa tataattttt
     1441 ttgaattatt tcaacactgt cggcatgatt ttttgctcca gctaactgat caaatgccga
     1501 ccagaaatac gtaatatcta cagtggaaat ttcaggtcga gtttgccctt gtaagaaaac
     1561 aggtaaaaat attacatgca aaagcactac acatgttttc atcaatagca attaattttc
     1621 agttgaaaat aaatattact tgacaggcta actattccgg tgctttacaa caagcatcta
     1681 atttgtcata tgcctgatgg tcagctttca tttcgtcggc atcgtaacca attgcaacaa
     1741 ttgctttttt gatttcctcc aatgcaattt tttcaggttt ataataaacc gttatcgtat
     1801 ttgcttttac atctattgca atatttttca caccatttaa tcccaacaag gtgtcatcaa
     1861 tactttcacc acaagtgcca cattccagac aatggtcgca aactatactc gaagcaatta
     1921 ctgcagtagc aacactgccg gtttgtgccg tggcaatatg cccaaaagcg attattgcaa
     1981 ttattgttat aatagatttc atctgatata ccatttatta tttttcaaaa ctacaggata
     2041 atgtttaccc aaacaaacct ttatcggttt cagttcaatt attacccaaa acgtcattta
     2101 ttcggcttac cttcaccgca accggtaacg ttctcatctt aatcgaaatt atgccctacg
     2161 aaaaactaat tcaatcctat gtagacaatc agttgggcat agatgtaaat tttatttccc
     2221 ctgaactggc actcagcctg aataatcgtt tggtggatgt atttaataca cataaaatgg
     2281 tgcaggcagg aaccggcaat aaggctgctg ttcatttcga ccaactatta agaggcgata
     2341 ccattttttg gctggaccgt gcacatgaaa atgcaagtga aaatgctttt ttggatatta
     2401 tcgatgcatt tgtcttgcac ctgaatgcca cttgttatgc aggaataaca ggatacgaat
     2461 ttcattttgc ccgatacgaa gaaggcagtt tttataccaa acatttcgac cgctttgaac
     2521 aaaat
//
LOCUS       DAORKW010000534         2525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2445_length_2525_cov_3.891093, whole genome shotgun sequence.
ACCESSION   DAORKW010000534 DAORKW010000000
VERSION     DAORKW010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2445_length_2525_cov_3.891093"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..589
                     /locus_tag="PLB46_15130"
     CDS             23..589
                     /locus_tag="PLB46_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF77910.1"
                     /translation="MMNSSPVSLKNTVVNLMFVGITAISLTVVGCAGAGSTAQHVGVA
                     TGNATPDIITESKNGEWLQLHALKGSYVLVEFWESGNSEARKNHFEMDRLYKKYKSAE
                     FKDGKNFCVYSVSLDTDKQKWLDALAQDNVSWPCQTIDTKSWNAKSALDFEVNFLPKY
                     YLVNGDGQIVKRNILIDDLEDILKAELN"
     gene            740..1273
                     /locus_tag="PLB46_15135"
     CDS             740..1273
                     /locus_tag="PLB46_15135"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRF77911.1"
                     /translation="MEEQEIKDEMQETAENQSANLPVEEAPVDETSSALKELEEQKDK
                     YIRLLAEFENFKRRTSKERIELFKTAGQEVIKDLLPSLDDVDRAEQVVAAAKDIEAVK
                     EGIKLISEKLKNTLSQKGLKEIPCMGIEFNTDLMEAITEIPAPSEDLKGKVVDVLEKG
                     YTLNDKIIRYAKVVIGK"
     gene            1305..2462
                     /gene="dnaJ"
                     /locus_tag="PLB46_15140"
     CDS             1305..2462
                     /gene="dnaJ"
                     /locus_tag="PLB46_15140"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRF77912.1"
                     /translation="MAKRDFYEILGVSKSASADEIKKAYRKMALQFHPDKNPGNKEAE
                     EKFKEAAEAYEVLSDPQKKAKYDQFGHAGVGGAGGGAHHMNMDDIFSQFGDIFGESVF
                     GDFFGGRGGGGGRGQRTRGIRGSNIRIKVKLTLKEIAEGARKTIKVKKYVVCDVCSGS
                     GAKDASSVSTCKTCNGAGVVRRVQNTILGQMATTQTCPTCHGSGQQVTASCNKCHGDG
                     RIYGEDTINIDIPAGVNDGIELSMNGKGNAGEKGGPNGDLLISIEEVKDNDLKREGNH
                     VLYDLYISFADAALGTKVEVPTIDGKVRVTIPEGTQSGHIMRLKGKGLPSLNSYGKGD
                     QIIEVHIWVPRKLSSEERKVLEKLQASKNFIPDINETRKEKGFFDKMKDMF"
BASE COUNT          882 a          396 c          573 g          674 t
ORIGIN      
        1 aaagcttaaa agattaaatt aaatgatgaa ctcctctcct gtatcactta aaaacaccgt
       61 tgtaaacctg atgtttgttg gtattacagc tatttctctc accgttgtag gttgcgccgg
      121 agcagggtct accgcacagc acgttggggt agcaaccggt aatgcaactc ctgacattat
      181 tacggaaagt aaaaatggtg aatggcttca actccatgca cttaaaggct catatgtact
      241 tgtcgaattt tgggaatccg gtaactcaga agcccggaaa aatcattttg agatggacag
      301 gttgtataaa aaatacaaaa gtgccgagtt taaagatggt aaaaactttt gcgtttacag
      361 tgtatcactc gatacggata aacaaaaatg gttagatgcg cttgcacagg ataatgtttc
      421 ctggccttgt caaaccattg ataccaaatc gtggaatgcc aagtcagcat tagactttga
      481 agttaatttt ctcccgaaat attacctggt aaatggtgac ggccaaattg taaaaaggaa
      541 tatccttatc gacgatttag aagatatttt aaaagccgag cttaattaag actgccgttt
      601 agctttttca cttaaaatat tgtgtcattt tggcaataac cgcccggtgg cagtattttt
      661 gcctccctca aatatcgtcc gccgaatggg gggaaggtga aactttgtca tttaccaaca
      721 ctttaatgag tagcaaaata tggaagagca agaaataaaa gatgaaatgc aggaaaccgc
      781 tgagaatcag tcagccaatc tcccggttga agaagctccg gttgacgaaa ccagttctgc
      841 attgaaggaa ctggaagagc aaaaggataa atacatccga ttactcgctg agttcgaaaa
      901 ctttaaacgc agaaccagca aagagcgcat tgaattattt aaaactgcag ggcaggaggt
      961 gattaaagat ttattgcctt cactcgacga cgttgatcgc gctgaacagg tagttgctgc
     1021 tgctaaagat atagaggcag ttaaagaagg gattaaatta atcagcgaaa aattaaaaaa
     1081 tacgctttca caaaaaggat taaaagaaat tccttgtatg ggcattgaat ttaatacaga
     1141 tttaatggaa gcaattaccg aaatacctgc tccaagtgaa gatttaaaag gcaaagttgt
     1201 tgatgtatta gaaaaaggtt atacgttaaa cgataaaata attcgctacg ctaaagtggt
     1261 aatcggtaaa taattcatca atttattaca aggacagaac ggacatggct aaaagagatt
     1321 tttacgaaat attaggtgta agcaaatcag cttctgcaga cgaaattaaa aaagcgtatc
     1381 gcaaaatggc attgcagttt cacccggata aaaatccggg caataaagaa gctgaagaaa
     1441 aatttaaaga agcagctgag gcatatgaag tattaagcga cccacagaaa aaagctaaat
     1501 atgatcagtt tggtcatgca ggtgttggcg gcgcgggtgg tggtgctcat catatgaata
     1561 tggatgatat tttcagccag ttcggtgata tttttggaga atctgtattt ggtgattttt
     1621 ttggaggacg cggcggtggc ggaggaagag gtcagcgcac aagaggaatt cgtggaagta
     1681 atattcgtat caaagtaaaa cttacattaa aagaaattgc agaaggtgca cgcaaaacaa
     1741 ttaaggttaa gaagtatgta gtttgtgatg tgtgttcagg aagtggtgct aaagatgcat
     1801 caagtgtaag tacatgtaaa acatgtaacg gcgcaggtgt agttcgtcgt gttcaaaata
     1861 ccatcttagg ccaaatggca acaacacaaa cttgtcctac ctgtcacggt tcaggacaac
     1921 aagttactgc cagttgtaat aaatgtcatg gtgatggaag aatttatgga gaggatacca
     1981 ttaatataga tatacctgca ggtgttaatg atggtatcga attatcaatg aatggcaaag
     2041 gtaatgcagg agaaaaaggc ggacctaatg gcgatttatt aatttcaatt gaggaagtaa
     2101 aagacaacga tttaaaacgt gaaggcaatc atgttttata cgatttatat atcagctttg
     2161 ctgatgctgc gttaggaaca aaagtggaag tgcctaccat tgatggtaag gttcgagtaa
     2221 caattccgga aggcacacag agtggtcata tcatgcgctt aaaaggcaaa gggttacctt
     2281 cactaaatag ttatggaaaa ggcgaccaaa taattgaagt acatatttgg gtgccaagaa
     2341 aattatcttc agaagaaaga aaagtattgg aaaaattaca ggcatcaaaa aactttattc
     2401 ccgatatcaa cgaaacccgt aaggaaaaag ggttttttga taagatgaag gatatgtttt
     2461 gataattagc aaattagctg attagcaaat tagctaatga ggtgtagtgc cggaatggaa
     2521 ttttg
//
LOCUS       DAORKW010000535         2525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2446_length_2525_cov_3.727126, whole genome shotgun sequence.
ACCESSION   DAORKW010000535 DAORKW010000000
VERSION     DAORKW010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2446_length_2525_cov_3.727126"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLB46_15145"
     CDS             <1..266
                     /locus_tag="PLB46_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HRF77913.1"
                     /translation="VIGVPVKSSNSIDGWDSILSILQMPNGVPVATVALNAAKNAGIL
                     AAEILGTSSIAIRNKIIAYKTSMQDTVIHKAKKIEKDGYKSHS"
     gene            complement(291..>798)
                     /locus_tag="PLB46_15150"
     CDS             complement(291..>798)
                     /locus_tag="PLB46_15150"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF77914.1"
                     /translation="KKVMILSSLFMLSLVFSSVRYLGNFGLENVYEAYLLAAVLSVPF
                     AVLGILPPVILAELTQADAYETKENREATFFAIRSLFIQFGQTMGIVIFTILIGLDQS
                     KGLGNYLAGVFSKVPFEELGIRLSGIFGFILCFIAAIIFSFFNEKKLKASIDAMEAEL
                     DEKAQQTT"
     assembly_gap    799..808
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<809..1703)
                     /locus_tag="PLB46_15155"
     CDS             complement(<809..1703)
                     /locus_tag="PLB46_15155"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF77915.1"
                     /translation="MKQTEKLPLRGQLIYASGTLGWSVVVNLLAGIIIYFYQPVGDDQ
                     LNNLIPKITILGFINALTLVVLSARLIDAIIDPVIAHLSDKSDNKLGRRIPFMIFALL
                     PVLVTGFLLWMPLTRTESMGNIWWLAGIQILFNVSISFYVIPYNALLPEFGYNSEVKL
                     RISTFQSIAYTIGLVIASASNALLNFNQDVLHIEDKFRAYQLAIWTIFVIGWIFLLIP
                     VFLKEKKYIVPNPVTTGIKDNFKTVLKNKNVLLYLIADFTYFISLTIIGTGALYYVKA
                     LLGLEEKHGTGMVATTVGLAMI"
     gene            complement(1746..>2525)
                     /locus_tag="PLB46_15160"
     CDS             complement(1746..>2525)
                     /locus_tag="PLB46_15160"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRF77916.1"
                     /translation="SEIAAMQNNTLPLWHFDTLHPQKAMFIFPHPDDEITCAGTIAKM
                     KANNWDVYLLTLTKGSDEQDKMVRTNEWNAAAKSLGYDKTLLCDFYNNSWNDIMQQNV
                     RFWPENLDSISSLIFNTVMDWQPDILITYDDAIGGYGHPEHLLSAKIVRDLFSTHLGN
                     TSFSPKYLYQMTLPQALEDFIVADLPSYKMAIEQYGLDGLPDPDLAVEITEFWPAKRN
                     AASSYQSQAEILNKFYLLPSNADTAAHYATFNKEYYREIKR"
BASE COUNT          823 a          473 c          461 g          758 t
ORIGIN      
        1 ctgtaattgg ggtgccggtt aaatccagca attcaatcga cggatgggat tcgatattgt
       61 ctattttaca aatgccaaac ggcgttccgg tagctactgt tgctttaaat gcggctaaaa
      121 atgcaggtat tcttgcggct gagatacttg ggacaagcag tattgctatc cgcaataaaa
      181 ttatcgccta caaaacgagc atgcaggata cggtaattca taaagcaaaa aagatagaaa
      241 aagacggcta taaatcacat tcctgagacc taattttctt ttattggttc ttatgtagtt
      301 tgttgtgctt tttcgtcaag ttcagcttcc atggcatcaa tcgatgcttt taattttttc
      361 tcattaaaga agctgaatat tatagctgcg ataaaacata aaataaatcc gaaaatacct
      421 gaaagcctta tacctaattc ttcaaaaggc actttactaa acacaccggc taaataattt
      481 cccaaacctt tcgactggtc caaaccaatt aaaatggtga aaatcacaat acccattgtt
      541 tgcccgaatt gaataaacaa actgcgaata gcaaaaaaag tagcttcgcg attttctttt
      601 gtttcataag catcagcttg cgtaagttcc gctaaaatta ccggaggtaa aatgcctaat
      661 acggcaaaag gaactgataa aactgcagct aataaatacg cttcataaac attttctaat
      721 ccgaaattgc ctaaatatcg gacacttgaa aaaaccaacg ataacataaa taatgaagat
      781 aaaatcatta ctttttttnn nnnnnnnnaa atcatagcca gtccaaccgt tgtagcaacc
      841 atgcctgtcc catgtttctc ttccagtccc agtaaggctt tcacgtaata caaagcaccg
      901 gttccaataa ttgtgagcga aataaaatac gtaaaatcgg caatgagata taacagtaca
      961 tttttatttt tcagcaccgt tttaaaatta tccttaatac ctgttgtaac aggattgggt
     1021 acgatatatt ttttttcttt gaggaaaaca ggaattaaaa gaaaaatcca accaataaca
     1081 aaaatggtcc atatagctaa ttgataagcg cggaatttat cttcaatatg caaaacatcc
     1141 tgattaaaat tcagtaatgc gtttgatgca ctcgcaatta ccaaacctat ggtataggca
     1201 atggattgaa atgttgagat ccgcaattta acttctgaat tatatccaaa ttccggtaat
     1261 aatgcattat aaggaattac ataaaatgag atagacacat taaataaaat ctgaataccg
     1321 gctagccacc aaatattgcc catactttcg gtgcgggtaa gtggcatcca caataaaaaa
     1381 cctgtaacta aaaccggtag taacgcaaaa atcataaatg gaattcggcg accaagttta
     1441 ttatcacttt tatcgctcaa atgtgcgatt acggggtcaa taatggcatc aattaatcgt
     1501 gcacttaata cgacaagggt aagtgcatta atgaacccga gaatggtaat ttttggaatt
     1561 aagttattga gttggtcgtc gccaacaggc tgataaaaat agatgataat gcccgcaagc
     1621 aggtttacca ccacgctcca tccgagtgtt ccggatgcgt aaatgagctg accacgaagc
     1681 ggtaattttt ctgtttgttt cacaaccaat tcttaaagag ggtgtaaaaa tagaacaatc
     1741 gtacatcacc gttttatttc acggtagtat tctttgttga aagtggcata atgtgccgct
     1801 gtatctgcat ttgaaggcaa taaataaaat ttgttaagta tttcagcctg ggattggtag
     1861 ctagaagcag catttctttt tgccggccaa aattcggtaa tttccactgc taaatcagga
     1921 tccggcagac catctaaacc atactgttcg atggccattt tgtaagaagg taaatcagct
     1981 actataaaat cttctaatgc ctgtggcagg gtcatttgat ataaatactt tgggctaaag
     2041 cttgtattcc ctaaatgggt agaaaacagg tcccgaacaa ttttagcact aagtaaatgt
     2101 tcgggatgtc catatccgcc gatggcatcg tcgtatgtaa ttaatatatc cggttgccaa
     2161 tccattacgg tattaaaaat cagtgaactt atcgaatcga ggttttcagg ccaaaaccgg
     2221 acgttttgtt gcatgatatc attccagctg ttattataaa aatcgcataa caatgtttta
     2281 tcgtaaccca aactttttgc tgcagcattc cattcattgg tgcggaccat tttatcttgt
     2341 tcatctgaac cttttgtgag tgtaagcagg tatacatccc aattgtttgc tttcattttt
     2401 gctatggttc cagcacaggt aatttcatca tcggggtgtg gaaaaatgaa catggctttt
     2461 tggggatgta atgtatcgaa gtgccataac gggagtgtat tgttctgcat agcagctatt
     2521 tcact
//
LOCUS       DAORKW010000536         2513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2464_length_2513_cov_4.649308, whole genome shotgun sequence.
ACCESSION   DAORKW010000536 DAORKW010000000
VERSION     DAORKW010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2464_length_2513_cov_4.649308"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1545)
                     /locus_tag="PLB46_15165"
     CDS             complement(232..1545)
                     /locus_tag="PLB46_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77917.1"
                     /translation="MRLLIFHILFFFCLIFEQNIYAQTTWVKHPSPVLERSATFPDWK
                     GLATGDPFVMLDNDTLKMWYSGVGWLSAFDDCPHVRIGYAWSLDGINWNEYANNPVLN
                     INPDTSMFDADGVETPTVIKDESAPPEQRYKMWYAGRKSRCLPINDHHFGYAYSPDGL
                     NWTKYEGNPVMSPGNPDEWFNTFISSPCVIYDEFTYKMWFTASDLVINGQETDGKGNI
                     GYAVSTDGINWTIYPYPIVEAGTQDNYDSVVVAEPSVVKIGSFYHLFYSMLNSWDIEN
                     FQVGYAIANDAITFTKSTLNPVLKIGEPGSWDAFWAAHPSVIYDETFGKFKMWYTGRD
                     KSEIVDLENYFWDIGYAESYMPNAITEHNENSVQVFPNPATNIIYVLNGTPGATVEIY
                     NLNGVLITTETMDYKFAIQISNLPQGIYFLNLPSAGNTKYTFIKL"
     gene            complement(1614..>2513)
                     /locus_tag="PLB46_15170"
     CDS             complement(1614..>2513)
                     /locus_tag="PLB46_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF77918.1"
                     /translation="DDLIVYPAHGAGSACGKNLSKETSDTLGNQKKTNSALRPGITKE
                     VFINELLNGLMPPPGYFPKNVLMNINGYDAIDDVMARGKKALSVTEFEVVANETHALI
                     IDTRAAQKFAKAFIPNSINIGINGSFAVWVGTLVPDIKQEILLITEPGREDEVITRLA
                     RVGYDNAIGYLDGGIDAWINAGKETETIPTVSVAEFAAAEVANPDINILDVRKKSEYD
                     SEHIVNAINAPLDYINNSMTAVDKNKTYYVHCAGGYRSMVFASILKARGYNNLIDVDG
                     GFASIKSSGKFKITDYVCPSTML"
BASE COUNT          800 a          487 c          415 g          811 t
ORIGIN      
        1 tttggtggta tacacgtaaa aattggttgt aaggggacta atttccagct gctgagcctg
       61 caccaaaccg gataacaagc ctataaaaac tattactata aatctcatcg cattaaatta
      121 tgggtacaaa cgaatagggc attatttata ttgttttgtt tgggacgaat gttacaaaac
      181 cggtttgcgg atgaccctta tcagaaaact aaaggttgat gctattcatc cttacaattt
      241 tatgaaagtg tattttgtat tacctgcaga tggaagattt agaaagtaga taccctgagg
      301 caaattactt atttgaatag caaatttata atccatagtt tcggttgtaa ttaaaacacc
      361 atttaaatta taaatttcaa ctgttgctcc gggtgtacca tttaaaacat aaattatgtt
      421 tgttgccgga tttggaaaaa cctgaacact attttcatta tgttctgtta ttgcatttgg
      481 catataagat tcagcataac caatatccca aaaataattt tctaaatcaa caatttcgga
      541 tttatcacgg ccggtatacc acattttaaa tttaccaaaa gtttcatcgt aaattacgga
      601 tggatgtgct gcccagaaag catcccagct tccgggttca ccaattttca gtaccggatt
      661 taaagtgctt ttagtaaatg ttatggcatc gtttgcaatt gcatatccta cttgaaagtt
      721 ttctatatcc caactgttta acatggaata aaaaagatga taaaacgaac ctattttcac
      781 cacacttggt tcggctacaa caactgaatc ataattatcc tgggttccgg cttcaactat
      841 cggatagggg taaatagtcc aattaatgcc atcagttgaa actgcatatc caatattacc
      901 cttgccatct gtttcctgac cattaattac taagtcgctt gcagtaaacc acattttgta
      961 agtaaactca tcataaatta cacaaggact ggaaataaaa gtgttaaacc attcatcagg
     1021 attgccgggc gacattaccg gattgccttc atattttgtc cagtttaatc catcaggtga
     1081 ataagcataa ccaaaatgat ggtcatttat tgggaggcac ctggattttc tacccgcata
     1141 ccacatttta tatcgttgtt caggcggtgc actttcatct ttaataaccg ttggtgtttc
     1201 tactccatca gcgtcaaaca tactggtatc cggattaata tttaatacag gattgtttgc
     1261 atattcattc caattaattc catctaatga ccatgcatat cctatccgga catgtggaca
     1321 atcgtcaaaa gcactaagcc aacctacgcc tgaataccac attttaagtg tgtcgttatc
     1381 taacatcaca aaggggtcgc cagtggctag gcctttccaa tcaggaaaag tggcactacg
     1441 ctctagcaca ggtgacgggt gttttaccca agtagtttgt gcgtaaatat tttgttcaaa
     1501 tatcaggcag aaaaaaaata atatatgaaa gattaataaa cgcatttaca aattaaaggt
     1561 aataaaaaaa tccacctaaa agatggattt ctttattact ctacaagata tgcttataac
     1621 atagtagatg ggcaaacgta gtccgtaatt ttaaatttac ctgacgattt tattgaagca
     1681 aatccaccat caacatcaat taaattgttg tagccgcgtg cttttaaaat agaagcaaac
     1741 accattgaac gatatccacc agcacaatgc acataatatg ttttattttt atctactgcg
     1801 gtcatgctat tattgatata atctaatgga gcatttatgg catttacaat atgttctgaa
     1861 tcatattcgc tttttttgcg aacatctaat atattaatat cgggattagc aacttcagct
     1921 gctgcaaatt ctgcaacaga aacagtagga attgtttctg tttctttacc tgcatttatc
     1981 caagcatcaa taccaccatc taaataacca atggcattgt cgtaaccaac acgcgccaat
     2041 ctggtaatta cttcgtcttc acgaccaggt tcagtaatga gtaatatttc ctgtttaata
     2101 tccggaacca aggttccaac ccaaacggca aaacttccgt ttataccaat attaatggag
     2161 ttaggaataa atgcttttgc aaatttttgt gctgcacgtg tatcaataat taaagcatgt
     2221 gtttcgttgg ctaccacttc aaattcggta acacttaatg cttttttacc acgcgccatt
     2281 acatcatcaa tggcatcgta gccattaata ttcattaaaa catttttagg aaaatatccc
     2341 ggaggtggca ttaatccatt taataattca ttaataaata cttcttttgt gatgcccgga
     2401 cgtaaagcgc tgtttgtttt tttctgattg cctagggtat cggaagtttc tttgcttaag
     2461 tttttaccac aagcactacc tgcaccgtgc gcaggatata caataaggtc atc
//
LOCUS       DAORKW010000537         2512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2467_length_2512_cov_4.952788, whole genome shotgun sequence.
ACCESSION   DAORKW010000537 DAORKW010000000
VERSION     DAORKW010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2467_length_2512_cov_4.952788"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLB46_15175"
     CDS             complement(<1..774)
                     /locus_tag="PLB46_15175"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRF77919.1"
                     /translation="MIKRNLSYYAAAILFFVVTALQPAKAANPDEGMWLANMVRQLNF
                     EYLKQLGLELSAEEIYNTENASLKDAVVQLYQGGSGFCTGELVSPNGLMFTNHHCGYE
                     AIVSQSTTEHNYIDDGFWARSYAEELPIPGLAIRILYDAKDITDSIAPKVEGLDMAQR
                     RAKVTEIQNRIIARYAEQGYEAEVKSMYYGNQYYVYLYQVYNDVRLTGAPPSSVGNYG
                     GDTDNWMWPRHTGDFSIFRIYADKSNNPAEYSADNVPFKP"
     gene            complement(891..1295)
                     /locus_tag="PLB46_15180"
     CDS             complement(891..1295)
                     /locus_tag="PLB46_15180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77920.1"
                     /translation="MRKLYTLLLVSAISTWSFASSEGLEGLSGEDCVMVSTEGTEGNE
                     DLSLSLNNDEGTVSIFPNPVKSFATISFTYENVDKISILNIVGREIKTVSPEPGKKEV
                     KVSLVDLQPGVYFLAAYSQGNTLITKKFLKEE"
     gene            complement(1444..>2512)
                     /locus_tag="PLB46_15185"
     CDS             complement(1444..>2512)
                     /locus_tag="PLB46_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRF77921.1"
                     /translation="LKLNYNDERVIHYGLIPRSHRGIFVINELPDLQARIQVALFNIL
                     QEGDIQIRGFRIRLPLDIQFVFTANPEDYTNRGSIVTPLKDRIESQILTHYPKDIATS
                     KIITHQEANINSTQNSSVQLNEIAKDLIEQIAFEARKSEYVDQKSGVSARMTITALEN
                     LVSGAERRALKSNEAKTFVRVSDFWSVIPSITGKIELVYEGEQEGPYIVAVNLIGKAI
                     RSQFTNYFPAPEKAKKPIGKKAETQQDPKRKNIYQEIIDWFNEGNTVDLLNESSAIDY
                     RRSLDRVPGLKKLVQKLHPGVAADEMYFLMEFVLHGLSEYSLLSKHLLHSGMQFSDLF
                     SSVFTDNPLAGLEEDDEDFTI"
BASE COUNT          761 a          496 c          426 g          829 t
ORIGIN      
        1 tggtttaaaa ggcacattgt cggcgctgta ttctgcagga ttatttgatt tatcagcata
       61 aattctgaaa atactaaaat caccggtatg acgtggccac atccagttat cagtatcacc
      121 accataatta ccaactgatg aaggcggagc tcctgttaaa cgaacatcgt tatacacctg
      181 atacaggtaa acataatatt gattaccata atacattgat tttacttcag cttcataacc
      241 ttgttcagca taacgggcaa ttattctatt ttgtatttcg gtaacttttg ctctgcgttg
      301 tgccatatcc aatccttcca ctttcggagc aattgaatcg gtaatatctt ttgcatcata
      361 taaaatgcgg atagctaatc ccggaattgg aagttcttct gcataacttc tagcccaaaa
      421 accgtcatca atataattgt gttcggttgt agactgagaa acaattgctt cgtatccgca
      481 gtggtgattg gtgaacatta atccgttagg agaaactaat tcgccagtac aaaaaccact
      541 tccaccctga tacaattgaa caactgcatc ttttaaacta gcgttttcgg tattatagat
      601 ttcttcagca gaaagctcta aacctaattg ctttaggtat tcaaaattta gttggcgaac
      661 catgttggct aaccacattc cttcgtcagg atttgctgct tttgcaggct gtaatgctgt
      721 taccacaaaa aataggatgg ctgcagcgta atagctcaaa tttcttttga tcatgatata
      781 atattatatg atttaaaatc cggtgcaaag gtagtaaaca aaagccttaa ggcggggcaa
      841 aaaacaaaaa aagcagccat tggctgcttt aaaaatatcg taaatattac ttattcttct
      901 tttaagaact tcttggtaat caatgtgtta ccttgtgaat aggcagccag gaagtaaacc
      961 cccggttgta aatcaactaa acttaccttc acttcctttt taccgggttc aggtgatact
     1021 gttttaattt ccctgccaac aatgttcagg atactgattt tgtcgacatt ttcgtaggta
     1081 aagctgatag tggcaaaact tttaaccgga tttgggaaaa tactaacggt gccttcatcg
     1141 ttgtttaaac tcagcgaaag gtcctcgttg ccttcagtac cttcggtaga taccataaca
     1201 caatcctcac cggataagcc ttctaacccc tctgaagagg caaaagacca ggtcgatatc
     1261 gcagagacca gtagtagtgt gtaaagttta cgcataggcg gttttcctaa aggtaaagat
     1321 ataaatttgt ttttagtata tcaaaatcaa aaaagtgtta ataaacgtat ttaacctgct
     1381 ttaacacatt tttgttccgt tattaggaca ggtttaacgt aaaaaggccc taaaggtttc
     1441 aggttaaatg gtaaaatcct catcgtcttc ctccaaacct gccaagggat tatcggtaaa
     1501 aacgctggaa aataaatcgc tgaactgcat acctgaatgc aataaatgtt tgctcaataa
     1561 cgaatattca gataatccat gtaaaacgaa ttccattaaa aaatacattt catcagcagc
     1621 aactccggga tgtaattttt gcacaagctt ttttaaccct ggcacacggt ctaaactgcg
     1681 acggtaatca atggcgctgc tttcatttaa caggtcaaca gtattccctt cattaaacca
     1741 gtcaattatt tcctgataaa tatttttacg ttttgggtct tgttgtgttt cggctttttt
     1801 accaattggt ttttttgctt tttcaggtgc cggaaaataa ttggtaaact gcgaacgaat
     1861 agcttttcca attaaattaa ctgcaactat atatgggcct tcctgttcac cttcatacac
     1921 taattcaatt ttaccggtaa ttgatggaat aacactccaa aaatcactta cacgcacaaa
     1981 cgtttttgct tcattacttt ttaatgcgcg acgttcagca cccgaaacta aattttctaa
     2041 tgctgttatt gtcatacgtg cactcacacc acttttttgg tctacatatt cactttttct
     2101 cgcctcaaaa gcaatttgtt cgattaaatc ttttgctatt tcatttaatt gcacactgct
     2161 gttttgtgtg ctattaatat ttgcttcctg atgcgtaata atttttgaag ttgcaatgtc
     2221 ttttggataa tgcgttaata tctgactttc aattctgtcc tttaacggtg taacaattga
     2281 acccctgttg gtataatctt cagggtttgc agtaaataca aattgaatgt ctaaaggtaa
     2341 acgaattctg aatccgcgaa tttgaatatc tccttcctgt aaaatattaa acaatgctac
     2401 ctgaattctt gcttgtaaat cgggcaactc gttaatgaca aaaataccgc gatgcgacct
     2461 cggaattaat ccgtagtgaa ttactctttc gtcgttataa tttaatttta aa
//
LOCUS       DAORKW010000538         2511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2469_length_2511_cov_4.914088, whole genome shotgun sequence.
ACCESSION   DAORKW010000538 DAORKW010000000
VERSION     DAORKW010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2469_length_2511_cov_4.914088"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PLB46_15190"
     CDS             complement(<1..876)
                     /locus_tag="PLB46_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77922.1"
                     /translation="MNRTVFQLLFAILLSLSTIPKTLGQLQCENDTSYLRSLIDLKTA
                     TYFDFQGGLYPDGVNIMPYAHNAAGMNIARQVLPLDSLGNLDLIDGKAGFICLGASTA
                     GNAFNHFKSVADADPTVNPCLKLVNCAVGAKGLEIMIDTVVNGWYWDTDVMSDLIDAN
                     LTRHQVQVVWLMVTSRIDTILTWPYQPFSVTAKYEALMPVLLAKFPNLKQIYLSGFNY
                     GGYADPSKEFYEMIVEPSSYWNNWSVKFLIENQIEGDSDLLYSGPARKSAWLAWGPHL
                     WADGMRANVTDGLKWN"
     gene            1038..1694
                     /locus_tag="PLB46_15195"
     CDS             1038..1694
                     /locus_tag="PLB46_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77923.1"
                     /translation="MNQSALVMEEKSNEHYLDDLKEIRSMMEKSSRFISLSGLSGISA
                     GIIALVGAFIASWYIKTHIENPSYTLQTVVMVQMEDVLFLLLDAGIVLFLALTSATYF
                     AVRKARMQNLPVWSKAGELTLINLLIPLATGAIFCLILYSYNIFILIAPATLLFYGLA
                     LLNASKYTLAEVRYLGICEIVLGLAGMIIPGYSIILWAIGFGLLHIIYGAVMYYRYER
                     "
     gene            1691..1990
                     /locus_tag="PLB46_15200"
     CDS             1691..1990
                     /locus_tag="PLB46_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRF77924.1"
                     /translation="MKDIIAGLNKEFDNRVRLGIMSILMVNEWVDFNTVKTLLNLTDG
                     NLASHISALEKTGYIEVKKEFIGKKPRTTYNATKEGKTAFRHHLDALEKLLKGNS"
     gene            2042..>2511
                     /locus_tag="PLB46_15205"
     CDS             2042..>2511
                     /locus_tag="PLB46_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77925.1"
                     /translation="MRLMSQLKDGFRLFIRWVEMYTFRAIVAALLIIASIILVIEPGW
                     MVAAVDSLRIPYAVFLVVFVMFFFLRNNYALASAGGIALLILAPGIWPYFKTASETPA
                     DVKAEKNETVVADFSVLHFNVKENNKKIATVAEAALASNADIVSMQELKENSFA"
BASE COUNT          774 a          471 c          510 g          756 t
ORIGIN      
        1 attccatttt aagccgtcgg tcacgttagc gcgcatgcca tcagcccata aatgtggccc
       61 ccaggccagc catgccgatt ttcgagcagg tccactgtat agcaagtcag aatcgccttc
      121 aatctgattt tcaattaaaa atttaaccga ccaattattc caataggaag acggttcaac
      181 aatcatttca taaaattctt tagaagggtc ggcatacccg ccataattaa acccactgag
      241 gtaaatctgt ttcaggtttg ggaattttgc taaaagcaca ggcatgagcg cttcgtactt
      301 ggcagtaacg gaaaaaggtt gatatggcca ggttaaaatg gtatcaatcc ttgaggttac
      361 catcaaccaa actacctgta cctggtgtct ggtaaggttg gcatcaatca aatcggacat
      421 tacatctgta tcccaatacc aaccattaac aacagtatca atcataattt ctaatccctt
      481 tgcaccaacc gcacagttga ctaatttgag gcaagggtta actgtcgggt cggcatcggc
      541 tacactttta aaatggttaa aagcattacc tgctgttgag gcacccaaac aaataaatcc
      601 tgctttgcca tctattaaat ccagattgcc taaactatcg agcggtaata cctgacgagc
      661 tatattcatc ccagctgcat tatgtgcata gggcataata ttgacgccat cgggatataa
      721 accaccctga aagtcgaaat aagtggcggt tttcaaatca attaaggacc gcaaataaga
      781 ggtatcgttc tcgcattgta actgaccaag tgttttagga atggtcgata aactgaggag
      841 aatagcaaaa agtaactgaa aaacggtgcg gttcattcaa attcaaaaat aaatcaaaaa
      901 gtactctatt acaactcaat aaaatcaaga ttgttgacag tcagagcaaa atcgactgca
      961 attacttgaa aaccagccga tatatttcat cacacacaat atgagataat tatatccggc
     1021 agaattatta taactttgtg aaccaaagcg ctttagttat ggaagaaaaa tcgaacgaac
     1081 attacttaga cgacctgaaa gagattcgct ccatgatgga gaaatcgtca aggtttattt
     1141 ctctaagcgg cttatccgga attagtgcag gtattattgc attggttggc gcttttattg
     1201 cgagttggta tatcaaaacc catattgaaa atccatcgta cactttacaa actgttgtaa
     1261 tggtgcaaat ggaagatgtg ctttttttgt tgctggacgc cggcattgta ttattccttg
     1321 cgctcaccag tgcaacttat tttgctgtgc gaaaagcaag aatgcaaaat ttgcctgttt
     1381 ggagcaaagc aggtgaactc acactcatta atctgctcat ccctttagca acaggtgcca
     1441 ttttttgttt gattttatac tcttataata tttttatact tattgcaccg gcaacattgt
     1501 tgttttatgg tcttgcttta ctcaatgcca gtaaatatac attggcagag gttcgatatt
     1561 tgggtatttg tgaaattgtt ttgggactgg ccgggatgat tatccccggt tattcgatta
     1621 ttttatgggc aattggattt ggattattac acattattta tggggcggta atgtattacc
     1681 gctatgagcg atgaaggata ttatagccgg attaaataag gaatttgaca acagggtccg
     1741 cttgggcatt atgagcatac tcatggtgaa cgagtgggta gattttaata ccgttaaaac
     1801 cctgctcaac ctgactgatg gtaatttggc cagtcatata tctgcacttg aaaaaaccgg
     1861 ttatattgaa gtaaaaaaag aatttattgg caaaaagccc agaactacct ataatgctac
     1921 caaggaaggg aaaactgcat tccgccacca tttagacgca ctggagaaat tgttaaaagg
     1981 aaacagttaa attttctaaa atatactttg aatttcaaag ttctttatta attttgtcgt
     2041 tatgaggctg atgagtcaat tgaaagatgg tttccgcttg tttatcaggt gggtcgagat
     2101 gtacacgttt agagcaattg tagctgcatt gctcataatt gcaagtatca ttctggtgat
     2161 tgaacccggc tggatggttg cagcagtaga cagcctccga attccttatg ccgtttttct
     2221 ggtggttttt gtcatgtttt ttttcctgag aaataattac gcacttgcct cggcaggtgg
     2281 aatagcgctt ttaatattgg caccgggcat ttggccttat tttaaaacag ctagtgaaac
     2341 acctgctgat gttaaagcag aaaaaaacga aacggtagtc gccgattttt cggtattaca
     2401 ttttaatgta aaagaaaata ataaaaaaat tgcgacagtt gctgaagccg cattggcatc
     2461 aaatgctgat atcgtttcta tgcaggaatt aaaagaaaat tcgtttgcca t
//
LOCUS       DAORKW010000539         2497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2492_length_2497_cov_5.792383, whole genome shotgun sequence.
ACCESSION   DAORKW010000539 DAORKW010000000
VERSION     DAORKW010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2492_length_2497_cov_5.792383"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PLB46_15210"
     CDS             <1..950
                     /locus_tag="PLB46_15210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRF77926.1"
                     /translation="ILIIASGCSIAPEETPGMKDKLWYKDIFDFYSFEGAKALHDKLK
                     NWEGGRLAIHITEMPIKCPVAPLEFAFLADDFFKHKDMRDEVEITYITPLSGAFTKPF
                     AAKKLAHLIEEKDIKLVTDFNVERIDNDEHKIIDYGGQEVKFDLLVTVPLNKGAAFIG
                     KSGFGDDLNYVPTDKFTLQTTVKENIFAIGDATSLPASKAGSVAHFESEILTENILHY
                     IKGEELKAHFDGHANCFVETGNHKALLIDFNYDQEPVEGTFPFAGVGPMSLMEETMLN
                     HMGKLGFKWIYWNVLMHGRSIPFIHPQMDKSGKKIPVNQ"
     gene            986..1297
                     /locus_tag="PLB46_15215"
     CDS             986..1297
                     /locus_tag="PLB46_15215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HRF77927.1"
                     /translation="MEKIINGVTIKVNEEGYLTDFSQWNKSIAEEISKQEGVGELGPK
                     HWEVLNFIQDQYKKSVPLTIRKIGASGVTDIKEFYALFPNGPLKKAAKIAGIPKPVGC
                     I"
     gene            1319..1840
                     /locus_tag="PLB46_15220"
     CDS             1319..1840
                     /locus_tag="PLB46_15220"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HRF77928.1"
                     /translation="MEDILMKEAPAESGVEQKKPIKKMCFILSKAGLESVYACFIMAN
                     GARMEGIEAEIFFTFFGLDAVHKKRLEHLHIATVGNPGMHIPTLLGGLPGMESYASRM
                     MRKQMEAMDLPTVHEFLDILSASGVQMYGCKLAADMFKITKDDLYEGIDSIITVGDFY
                     QHAQGDGVHMIFV"
     gene            complement(1898..>2497)
                     /locus_tag="PLB46_15225"
     CDS             complement(1898..>2497)
                     /locus_tag="PLB46_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HRF77929.1"
                     /translation="IVQQMSLASSAYAKGVNEFEKAGLTPIPSELVKPFRVKESIVQM
                     ECKVRDIIRTGETGGAGNIIICEIVLMHVDESILDENGRPDPYKMDLVARMGGEYYCR
                     VIPESIFALAQPKDGSALGIDQLPEYIKNSSILTGNNLGQLGVFAHHPTKEEVESIAH
                     LMRHHMSWQEIELQAKTLLDKGEAWEGFKVLMLKSFQLV"
BASE COUNT          807 a          434 c          466 g          790 t
ORIGIN      
        1 atatattaat tatcgcttcc ggttgcagta ttgctccaga agaaactccc ggtatgaaag
       61 ataaactttg gtataaagat atcttcgatt tttattcatt tgaaggagcc aaagcattgc
      121 atgataaatt aaaaaattgg gaaggcggac gtttggcaat tcacattaca gaaatgccaa
      181 ttaaatgtcc tgttgcccca ttagaatttg catttttagc cgacgatttc tttaaacata
      241 aagatatgcg tgatgaagtg gaaattactt acatcactcc attaagcggc gcatttacta
      301 aaccatttgc tgcaaaaaaa ttagcccact taattgaaga aaaagacatc aaactggtca
      361 ctgattttaa tgttgaacgt atcgataacg atgaacataa aattattgat tatggtggtc
      421 aggaagtgaa attcgactta ttagttactg ttccattaaa taaaggtgca gcatttattg
      481 gtaaatccgg ttttggcgat gatttgaatt atgtgcctac tgataaattt actttacaaa
      541 ctacagtgaa agaaaatatt ttcgcaattg gtgatgctac cagtttacca gcctcaaaag
      601 ccggttcagt ggcgcatttc gaatcggaaa ttctcaccga aaatatttta cattatatta
      661 aaggagaaga gttaaaagcc catttcgatg gtcatgcaaa ttgttttgtt gaaaccggta
      721 atcataaagc attattaatc gatttcaact acgatcagga accggtagaa ggtactttcc
      781 cattcgctgg tgttggacca atgtcgctga tggaagaaac tatgctcaat catatgggca
      841 aacttggatt taaatggata tactggaatg tgttaatgca cggtagatca attccattta
      901 ttcatccaca aatggacaaa tcaggtaaaa aaattcctgt taatcaataa aaccacatat
      961 taatttaatt ctttaaaagt tttttatgga aaagataatc aatggtgtta ccatcaaagt
     1021 aaatgaggaa ggttacctca ctgacttttc gcaatggaat aaatctattg ctgaagaaat
     1081 ttcaaaacaa gaaggtgttg gcgaattggg accaaaacat tgggaagtat tgaattttat
     1141 tcaagaccaa tacaaaaaaa gtgtcccttt aaccattcgt aaaattggtg caagtggcgt
     1201 aacagatata aaagaatttt atgcactgtt cccaaatgga ccacttaaaa aagccgctaa
     1261 aattgcgggt attcctaaac cagtaggttg tatttaattc acttgtaaaa attattttat
     1321 ggaagatatt ttaatgaaag aagctcctgc cgaaagcgga gttgagcaaa aaaaacccat
     1381 caaaaagatg tgttttatat tatcaaaagc cggactcgaa agtgtatacg cttgttttat
     1441 catggctaat ggcgcacgta tggaaggtat tgaagcggaa atatttttca ccttcttcgg
     1501 tttagatgca gtgcacaaaa aacgccttga acatttacac atcgcaactg taggtaatcc
     1561 cggtatgcat attccaacat tgttaggtgg attacccgga atggaaagtt atgcttcacg
     1621 tatgatgcgc aaacaaatgg aagcaatgga tttgccaaca gtgcatgaat ttttggatat
     1681 tttatctgcg tccggagttc aaatgtacgg atgtaaactc gctgccgata tgtttaaaat
     1741 tacaaaagac gatttatacg aaggtattga cagtattatc actgtcggcg atttctatca
     1801 acacgcgcaa ggtgatggcg tgcatatgat ttttgtttga tgattaataa gctgtaagct
     1861 gaaaggtcgg tgttgagccg acctttttta ttatttttta tacaagttga aacgatttta
     1921 gcatcagcac tttaaatcct tcccaggctt ctcctttgtc aagtaaagtt tttgcttgta
     1981 attcaatttc ctgccagctc atgtggtgtc gcattaaatg agctattgat tcaacttctt
     2041 cttttgtagg atgatgggca aatacaccta attgtcctaa attatttccg gttaaaatag
     2101 atgaattttt aatgtattca ggcaattgat ctatgcctaa tgccgaacca tctttaggct
     2161 gagctaatgc gaaaatagat tctgggatta cgcggcaata atattctcca cccatacgtg
     2221 caactaaatc cattttgtaa ggatcgggtc tgccattttc gtctaatatc gattcatcaa
     2281 catgcattaa aacaatttca caaataataa tatttcccgc accacctgtt tcgccggtgc
     2341 gtataatatc gcgtacctta cattccatct gcacaatact ttcttttacc cgaaatggtt
     2401 taactaattc tgatggaata ggcgtgaggc ctgctttttc aaattcgttt acaccttttg
     2461 catatgctga actggctaaa ctcatttgct gcacaat
//
LOCUS       DAORKW010000540         2491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2502_length_2491_cov_4.342365, whole genome shotgun sequence.
ACCESSION   DAORKW010000540 DAORKW010000000
VERSION     DAORKW010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2502_length_2491_cov_4.342365"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..616
                     /locus_tag="PLB46_15230"
     CDS             233..616
                     /locus_tag="PLB46_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRF77930.1"
                     /translation="MKNVVFTPSAPAPIGPYSQAIEAGGFVFVSGQIAIDPVTGNLYS
                     GDDIQAETRVVMENLKAIILQAGCSMKDVVKCTIFLTDMNNFAQVNTVYGEYFPEYAP
                     ARETVAVVGLPKNVRVEISAIVVKR"
     gene            complement(629..1531)
                     /locus_tag="PLB46_15235"
     CDS             complement(629..1531)
                     /locus_tag="PLB46_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRF77931.1"
                     /translation="MQKYSTTNWLLLIVLSLIWGFSFIFMKYGLKTFSWDEVAAIRIS
                     FSFIATLPILIFYFKQIKKSEIKYYCMVGFFGSGLPAFCFTFSQTHIESGIAGVLNSL
                     TPVFTFVLGVLFFTMKFEKNKLYGLIVALTGAIILVYFDKAPGGESNLIYALPLFVAT
                     MSYATSANLVKRYLQNAHPLTLGAVGFSFIGIPAIIYLFTTDFLQHSHDEYFTISLSS
                     ILALSFFGTVIASIGYYTLIQRTDAIFGSLVTYLIPIVAILIGFIDGEKLFFYHLIGM
                     LFILAGIYIINYKPKGQASEKALK"
     gene            complement(1545..2474)
                     /gene="obgE"
                     /locus_tag="PLB46_15240"
     CDS             complement(1545..2474)
                     /gene="obgE"
                     /locus_tag="PLB46_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRF77932.1"
                     /translation="MHVHRAKDTRKGGPDGGDGGRGGHIILRGNAQLWTLLHLKYRKH
                     VIAENGQGGTERLRTGSEGADVILEVPLGTVAKDFETDEVEFEIVEDGQEVIWVPGGR
                     GGLGNNHFKSPTNQTPRHAQPGEPGQEAWKLLELKVLADVGLVGFPNAGKSTLLSVLS
                     AAKPEIADYPFTTLVPNLGIVAYRGHKSFIMADIPGIIEGASDGKGLGIRFLKHIERN
                     SLLLFMVSCEDKDIKTTFKILLNELKTFNKELLSKQRILAITKSDLIDDELEKMLKKE
                     LPKNIPTIFISSHTQKNLDKLKDMIWTELNKPV"
BASE COUNT          793 a          497 c          436 g          765 t
ORIGIN      
        1 aaattccaac tattaccaaa gctaatatga gattaaaata acggcgcata ccaattgtgt
       61 gcttgatttt ggttgatgtt tacgctaaaa gaggtataaa tttacaaaca atataaccaa
      121 atccaaatcg gataacatga acaattccaa acctttaagc ggtttaaacg ctttttttag
      181 catttgtgcg atagtaatta cctttgaagc ctgttcacca aaacaagttt ttatgaaaaa
      241 tgtagtgttt accccttcag ctccggcacc aatcgggcct tattctcaag ctattgaagc
      301 gggcggtttt gtatttgttt cgggccaaat tgccatcgac ccggttacag gaaatctgta
      361 ttccggcgat gatattcagg ctgaaacccg cgtggtaatg gaaaatttga aggcaattat
      421 tttgcaggct ggttgtagca tgaaagatgt agtgaagtgc actatatttt taaccgacat
      481 gaataacttc gcccaggtga acactgtata cggtgaatat ttccctgaat atgctccagc
      541 acgtgaaacg gttgcagtgg tggggctacc taaaaatgtg cgagtggaaa ttagcgcaat
      601 tgtagttaag cgttaatgtc ggatttcctt acttgagagc tttttctgaa gcttggccct
      661 ttggcttata gttaattatg taaatgcctg ccaaaataaa taacatacct atcaggtggt
      721 agaaaaaaag tttttctccg tcaataaatc caattaatat agcgactatc ggaatgaggt
      781 aagttaccag actgccaaat atagcatctg ttcgttgaat aagtgtataa tatcctattg
      841 atgctattac agtcccaaaa aaacttaagg ccaaaatact gctcagacta attgtaaaat
      901 attcgtcgtg gctatgttgt aaaaagtcgg ttgtaaacaa atatataatg gccggtatac
      961 caataaatga aaaacctaca gcgcctaatg ttaaaggatg agcattttgt aaataccttt
     1021 ttaccaagtt agcagaagtg gcataactca tggtggcaac aaaaagtggt aaggcataaa
     1081 ttaaattact ttctccaccc ggggctttat caaaatacac caaaataata gcacccgtta
     1141 acgctacaat caatccgtat aatttatttt tttcgaattt catagtgaaa aaaagtacac
     1201 ctaaaacgaa agtaaatacc ggcgttaaag agtttaacac ccccgcaata cctgattcta
     1261 tatgggtttg agaaaatgtg aaacaaaagg caggtaagcc acttccaaaa aagcctacca
     1321 tacaataata ttttatttcc gattttttta tttgtttaaa atagaaaatt aaaataggta
     1381 aagtggcaat aaaagaaaat gaaattcgga tggcagcaac ttcatcccaa ctgaatgttt
     1441 ttaatccgta tttcataaaa ataaatgaaa aaccccagat taaactcaat acaattaata
     1501 gtaaccagtt ggtagttgaa tatttctgca taaaaaaaat aatactaaac aggcttattt
     1561 aattctgtcc aaatcatatc ttttaatttg tccagatttt tttgcgtatg cgacgaaata
     1621 aaaatagtag gaatattttt gggcaattct tttttcagca ttttttctaa ttcatcatca
     1681 attaaatctg attttgttat tgccaaaatg cgttgcttac tcaataattc tttgttaaat
     1741 gttttgagtt cattcaacaa aattttaaag gttgttttga tatccttgtc ctcgcagctc
     1801 accataaaaa gtaagagtga attacgttca atatgtttca aaaagcgtat ccccaaacct
     1861 ttgccatcgc tggcaccttc aataattccg ggaatatcgg ccatgataaa cgatttatgt
     1921 ccccgataag ccacaatacc caggttagga accaatgtag taaaaggata atcggcaatt
     1981 tcgggttttg ctgcagataa aactgaaagt aacgtcgatt taccggcatt tggaaaacct
     2041 accaaaccca catctgccaa cacttttaac tcgagtagct tccaggcttc ttgtccgggt
     2101 tcaccgggtt gtgcatgtcg gggcgtttga ttggttggtg atttaaaatg gttattacca
     2161 aggccacctc ttcctcccgg cacccaaatt acttcttgtc cgtcctcaac aatttcaaat
     2221 tcaacctcat cggtttcaaa atctttggca actgttccta atggcacttc taaaattaca
     2281 tctgcgcctt ctgaccctgt tctcaacctt tctgttccac cttggccgtt ttccgcaatc
     2341 acgtgtttgc gatatttcag atggagtaat gtccacaatt gagcattgcc tcttaatata
     2401 atatgaccgc cacgcccacc atcaccaccg tcgggcccac ctttcctggt gtctttagct
     2461 ctatggacat gcacggctca tccaccaccc t
//
LOCUS       DAORKW010000541         2482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2516_length_2482_cov_5.061805, whole genome shotgun sequence.
ACCESSION   DAORKW010000541 DAORKW010000000
VERSION     DAORKW010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2516_length_2482_cov_5.061805"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /gene="mltG"
                     /locus_tag="PLB46_15245"
     CDS             <1..481
                     /gene="mltG"
                     /locus_tag="PLB46_15245"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRF77933.1"
                     /translation="HDNFWTPTRRDKAEKIGLTPEQVYILASIVYSENDKAADEAPRI
                     AGVYMNRINKGMKLEADPTVKFAIGNFAIKRILFADLEIESPYNTYKHVGLPPGPIHM
                     PPIAYIDAVLNYEHHEYIFMCANGDGSGYHLFAKTLAEHNRNRDIYIATLNKQGIRR"
     gene            complement(478..945)
                     /locus_tag="PLB46_15250"
     CDS             complement(478..945)
                     /locus_tag="PLB46_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF77934.1"
                     /translation="MMKPIAARLLTIVDEFAIKFQQIAPSIFDEKPHPEKWSKKEIVG
                     HLIDSAQNNLRRFIVGQYQTHDKIIYQQDFWVAANNYQKMPQSSIIELWQLLNHRIVA
                     ILEEMPSGYYTNVIDTGKESINTHTLLWLAEDYIQHLQHHVNQIIPGSFDVNY"
     gene            complement(989..1561)
                     /locus_tag="PLB46_15255"
     CDS             complement(989..1561)
                     /locus_tag="PLB46_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77935.1"
                     /translation="MQEIEPFYSWREYYASEEDELSPFFGREYNEFEFTNTIYNYFIH
                     PQWDEFGSATLYLKIIFADYEAGVVIMEFIGEWNDCLYNDIMFLKRDVIDELTRNGIT
                     KFILIGENVLNFHGGDEDYYEEWLDDIEDGWIAAINFREHVEDEMIRAHVDRYMLIGE
                     PFNNLNWRKYKPQALALLVETVMQKRLGVG"
     gene            complement(1650..2456)
                     /gene="murQ"
                     /locus_tag="PLB46_15260"
     CDS             complement(1650..2456)
                     /gene="murQ"
                     /locus_tag="PLB46_15260"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HRF77936.1"
                     /translation="MQKTTEQTSNYRHLEKMSTSELLSNINTEDKLVPLAIEKVIPTM
                     EKLIDEIADRMLSGGRLFYIGAGTSGRLGIVDASECPPTFGVPQGLVIGLIAGGDAAI
                     RKAVEHAEDDTTQGWKDLEAHQITTNDTVIGIAASGTTPYVVGALKTCRENNILTGCI
                     VCNVGSPVAAHADYALEVVVGPEFVTGSTRMKSGTAQKLCLNMISTAVMIKLGRVEDN
                     KMVNMQLSNYKLIDRGTKMIVDQTGLSYDAANALLLKEGSVKKALIAFGK"
BASE COUNT          757 a          504 c          413 g          808 t
ORIGIN      
        1 acacgataat ttttggacac cgacacgaag agataaggca gaaaaaatcg gattgacgcc
       61 ggagcaggtt tatattttag cgtccattgt ttattctgaa aacgacaaag ctgcggacga
      121 agcaccaaga attgccggtg tgtatatgaa cagaattaac aaaggcatga aactggaggc
      181 agacccgaca gtgaaatttg ccattggcaa tttcgccatc aaacgtattt tatttgctga
      241 cctggaaatt gaatcgccat ataataccta taaacatgtg ggattacctc cggggccaat
      301 tcatatgcca ccaattgcgt atattgatgc tgtgttgaat tatgaacatc atgaatatat
      361 ttttatgtgc gccaacggtg acggttcagg atatcacctt tttgcgaaaa ccttagcaga
      421 acataatcgc aaccgcgata tttatattgc tacattaaat aaacagggca tacgacgtta
      481 ataatttacg tcaaatgagc ccggaattat ctgattaaca tgatgttgta aatgctggat
      541 ataatcttca gccaaccata ataaggtatg ggtattgatg ctttcttttc cggtatcaat
      601 tacattggtg tagtagcctg aaggcatttc ttccaaaatt gcgactatgc gatgattgag
      661 taattgccat aattcaataa tagatgactg tggcattttt tgataattgt tagctgcaac
      721 ccaaaaatcc tgctgataaa taattttatc atgggtttga tattgcccta ctataaacct
      781 gcgtaaattg ttttgtgctg aatctatcag atgaccaaca atttcttttt tgctccattt
      841 ttcgggatga ggtttttcat caaatataga aggtgctatt tgttggaatt taatagcgaa
      901 ttcatcaaca attgtaagca agcgagctgc aattggtttc atcataataa tttagtttaa
      961 ctattgccag gaaattaatc tccccaactt aacctacgcc aagccttttt tgcatcacgg
     1021 tttcaacaag taatgcgagt gcctgtggct tatattttct ccaattgagg ttgttaaatg
     1081 gttcaccaat cagcatatat ctatcgacat gtgcacgaat catttcatct tccacgtgtt
     1141 ctctgaaatt gattgccgca atccagccat cttcaatatc gtcgagccat tcttcataat
     1201 aatcttcatc accaccgtga aagtttaaca cattttcgcc aatgagtata aatttagtga
     1261 tgccattacg ggttagttca tcaataacat cccgttttaa aaacataata tcattataca
     1321 ggcaatcgtt ccactccccg ataaattcca taataactac tcctgcctca taatcggcaa
     1381 aaattatttt tagatataat gtcgcagaac caaattcatc ccattgagga tgaatgaaat
     1441 agttataaat agtattcgta aattcaaact cgttgtattc ccttccgaaa aaagggctca
     1501 attcatcttc ctcagaagcg taatattcac gccaactata aaatggttct atttcctgca
     1561 tataccttgt aacgccggaa aattattttg tatttcggaa agaaaaaaat ttattttatg
     1621 cagaaactac caaattaatt tggtaaactt cactttccaa aagctattag tgcttttttc
     1681 acactgcctt ccttcaacaa aagggcatta gctgcatcat acgataaacc ggtttggtca
     1741 acaatcattt ttgtaccgcg gtctattaat ttataattac ttaactgcat gttaaccatt
     1801 ttattatctt caacgcgacc taattttatc atcacagcag ttgaaatcat atttaaacat
     1861 aatttttggg ccgtaccact tttcattctg gttgaacctg tcacaaattc gggaccaacg
     1921 acaacttcca atgcataatc tgcatgtgct gcaaccggag aaccgacatt acaaactata
     1981 catccggtaa gtatattatt ttcgcgacat gtttttaatg caccaacaac atagggtgta
     2041 gtgccacttg ctgcaattcc aattacagta tcattggtgg ttatttgatg tgcttctaaa
     2101 tctttccagc cttgtgttgt atcatcttca gcatgttcta ctgctttgcg aatggctgca
     2161 tcaccaccgg caattaatcc aataaccagt ccctgaggaa caccaaatgt tggcggacat
     2221 tcacttgcat ctactatacc taatcttccg gatgttcctg caccgatata aaataaacgt
     2281 ccaccggata acattctgtc cgcaatttca tcaatcagtt tttccatggt gggaattacc
     2341 ttttcgatgg ccaaagggac caatttatcc tcagtattga tattcgataa taattcacta
     2401 gtagacattt tttccaaatg tcggtaattc gaagtttgtt ctgttgtttt ttgcatatta
     2461 ttaatttcac cacctattta ag
//
LOCUS       DAORKW010000542         2480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2519_length_2480_cov_5.277113, whole genome shotgun sequence.
ACCESSION   DAORKW010000542 DAORKW010000000
VERSION     DAORKW010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2519_length_2480_cov_5.277113"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1765)
                     /locus_tag="PLB46_15265"
     CDS             complement(224..1765)
                     /locus_tag="PLB46_15265"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRF77937.1"
                     /translation="MNKRYSLFLLLVLSALTIWSQNSDHPYKFGFGTSLIDYNAESES
                     YFKDLTDTGDIEFGPAVSRYTFGGTLNKSFSLGAAVSLNSIKVNAKTGNTSSSLFVDG
                     EINLNYHLANDYILKESSCFAPYVFGAVGVNYLENENPDVETIFPEGKLGIGTDIWVT
                     PLFGFNLQSAYVRQFNEQGNDYAHHSIGLVIRFGKGADNDGDGIPNWEDACPDKAGII
                     QFQGCPDTDNDGITDAADACPNQAGLALFQGCPDSDSDGLADKDDSCPNEKGLVAFNG
                     CPDSDSDGIMDKEDRCPQDKGTAAFKGCPDSDGDGIVDLDDNCKNEKGLSQFNGCPDS
                     DGDGIMDKEDRCPKERGELALQGCPDSDGDGTADIDDRCPDKRGIKANGGCPVLDDVE
                     RKKIVEKINYAAKSIQFESGSDVIKASSYSTLDNIVSIMTLYPTTAWSIEGHTDDQGD
                     DKMNQELSDKRAAAVRKYFITKGVADTRLSSAGFGETTPIADNKTSAGRAQNRRVEIK
                     LVEQK"
     gene            1946..2431
                     /locus_tag="PLB46_15270"
     CDS             1946..2431
                     /locus_tag="PLB46_15270"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF77938.1"
                     /translation="MERIKCLIIGSGPAGYTAAIYASRANLKPVMYTGIEPGGQLMQT
                     TDVENFPGYPTGIMGPEMMEDFKKQAERFGTDIRFGIATKVELKGDIKVVHFDDGTSV
                     EAYTVIIATGASAKWLGLPSEKRLNGNGVSACAVCDGFFFRNQEVALVGGGDTACEEA
                     L"
BASE COUNT          768 a          513 c          438 g          761 t
ORIGIN      
        1 tcatcatcgg caaaggcggc atcgccatta aaaaaacagg tactgaagcc cggatagata
       61 ttgaggcatt tactgaacgt aaaatatttt tggaactatt tgttaaagtg aaagaaaact
      121 ggaagaacga agatcggact ttaaaatatt ttggatataa ttaaaacaaa aaaaagggaa
      181 ttaccacaca agtaattccc ttttttattt ttaatatgct ctattatttt tgttccacca
      241 atttaatttc tactcttctg ttttgtgcac gtcctgcaga agttttatta tcagcaattg
      301 gtgttgtttc accaaaaccc gcactgctta atctggtatc agctacacct tttgtgataa
      361 aatattttct caccgcagcg gcgcgtttat cagataattc ctgattcatt ttatcatcac
      421 cctgatcgtc agtatggcct tcaattgacc atgctgttgt tggatataag gtcataatac
      481 ttacaatatt atccaaggta gaatacgatg aagctttaat tacatcgcta cctgattcaa
      541 attgaattga tttagctgca taattaattt tctccactat ttttttccgt tcaacatcat
      601 ctaaaaccgg gcatccgcca ttggctttaa ttccacgttt atcaggacaa cggtcatcaa
      661 tatcggcagt gccatctcca tcgctgtcag ggcatccttg taatgctaat tcacctcgtt
      721 ctttcggaca acggtcttct ttatccataa taccatcacc atcgctatcg ggacaaccat
      781 taaattgtga aagccctttt tcatttttac aattatcatc taaatcaaca ataccatctc
      841 cgtcgctatc agggcatcct ttaaatgctg ctgttccttt atcctgaggg caacgatctt
      901 ctttatccat aataccatcg ctatcactat caggacaacc attaaatgcc accaatcctt
      961 tttcattagg gcaactgtcg tctttatctg ccaaaccatc actatcgcta tcgggacatc
     1021 cctggaataa agccaaccct gcctgattag gacatgcatc ggctgcatcg gtaataccat
     1081 cattgtcggt atcgggacaa ccctgaaatt gaataatacc tgctttgtcc ggacaagcat
     1141 cttcccagtt tggaatgcca tcgccatcgt tatctgcacc ttttccaaaa cgaattacta
     1201 aacctattga atgatgtgca tagtcgtttc cctgttcgtt aaactgacgc acataagcag
     1261 attgtaaatt gaatccaaac aaaggtgtaa cccaaatatc tgtgcctata cccaatttac
     1321 cttccgggaa aatggtttca acatccggat tttcgttttc aagatagtta acccccactg
     1381 caccaaaaac ataaggagca aaacaactgc tttctttaag gatataatcg ttcgcgagat
     1441 ggtagtttaa atttatttcg ccatcaacaa ataaactgga acttgtatta ccggttttag
     1501 cgtttacttt aatggagttt aaacttacag cagcacctaa cgaaaatgat ttatttaatg
     1561 ttccgccaaa tgtatatcgg cttacagcag gtccgaattc gatatctccg gtatcagtta
     1621 aatctttaaa atacgattct gactcggcat tatagtcgat taaactagta ccaaaaccaa
     1681 atttataggg gtgatcggag ttttgagacc aaattgtaag cgcactaagc actaacaata
     1741 aaaaaaggga gtaacgtttg ttcatagtag ttgaatttga ggttaaatta gaacgataaa
     1801 tatatgttaa ttaaaattaa taccaccttt attaaagata aagttttaca cccaaaatga
     1861 tttttttgca aaagaaaaac aggattataa attgtgcgca tgttatttaa ctttgcacac
     1921 ctttaaccca atacaatacc ataaaatgga acgaataaaa tgtctcataa tcggctcagg
     1981 acctgcaggt tataccgcag ctatctatgc atcccgggca aatctaaaac ctgtaatgta
     2041 caccggcatc gaacccggag ggcagttgat gcaaacaaca gatgttgaaa attttccggg
     2101 ttatccaacc ggtataatgg gacctgagat gatggaagac tttaaaaaac aggctgaacg
     2161 ctttggcacc gacatccgtt ttggtattgc gaccaaagtt gagttgaaag gagatattaa
     2221 agtggttcat tttgatgatg gtacttcagt tgaagcctat accgtaatta ttgccaccgg
     2281 tgccagtgca aaatggcttg gattaccatc agaaaaacgt ctgaatggaa atggtgtaag
     2341 tgcatgtgcg gtttgtgatg gatttttctt ccgcaatcag gaagttgctt tagttggtgg
     2401 tggagatacg gcttgtgaag aagcattata attttctaaa ttgtgcgaaa acgtacacat
     2461 tccggtacgc acacacccat
//
LOCUS       DAORKW010000543         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2528_length_2474_cov_4.531211, whole genome shotgun sequence.
ACCESSION   DAORKW010000543 DAORKW010000000
VERSION     DAORKW010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2528_length_2474_cov_4.531211"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            537..1838
                     /locus_tag="PLB46_15275"
     CDS             537..1838
                     /locus_tag="PLB46_15275"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HRF77939.1"
                     /translation="MLKIHTLILWLIVSISIKAQISLEGLFDEPKQTADTLYFPFEDG
                     IQLIVINYYDTWERQVAFKEAMQPRGLSWAAIMGPAPKNMYIFRNTEGKIVKTYNSVS
                     DGKGLKGMDYDNTGIFLLQGLRDFTEYSRHLKYYDLNDQVGLINTRGDIVVPAVYDDI
                     RRYQDFDGEWEKLIIEKDGYFGFLDSNLNVLFPPIYRCSSDTNYYGYPEHNILNGQYI
                     KVFKEGKCGLINDHGDVLIDFKYDNLLLIHDSMIVGLLNKKLINPNQYFWNNVQACEI
                     YDDKFNLITSLDEYDQIEYNGIKTFIVKKDNLVGVVNHKGKVIIPIEYNSLAPQDNNY
                     YVVKGNKSGVISLEGKVLIPIEYECNMYFYEPAIYVTQDGLIGVYSDNYQLIAEPQYK
                     QRYWEMGKFMLIRPDGSIGFVLHQKGGSYYQSPEGEVIKFD"
     gene            1953..>2474
                     /locus_tag="PLB46_15280"
     CDS             1953..>2474
                     /locus_tag="PLB46_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77940.1"
                     /translation="MKIAGYVFILLSFAACNNKNTSVKSAANIDSLNDENIDNVSRDS
                     PESYLHTEKKFTDSSGLNITIQNSFPKGGPFYSNTGSQFGCAVFWSRFINESTKPVEL
                     TINFPADSFIVFKSTDAYLKLLIPSDTMSLEKVGLYNYGATGLQTFLEANFHKATGLH
                     QIIPSGDDFIFYTT"
BASE COUNT          823 a          372 c          479 g          800 t
ORIGIN      
        1 atcgggggtt taatttttta taaaagcccc cacctttggt gggggttgtt tacttacagc
       61 acattataaa gaaagttgaa tttaatttat tggagcatta acatcccgct tcgaatcaaa
      121 atcgttgccg gtgttctgct acaagtcctc gcccgcttta aggcgcgctg cgggctttcc
      181 tcccgatagc tatcatgaca cccctagcta gaagaactga aataattatc caacaagcct
      241 tcttaattaa tagttatggc atatataatt cctttcaaaa agagtttaat gcgattagga
      301 tgatttctat tttgttacac aaaaacacaa aagaaaaaca cgcaaaagac acaaaaaaag
      361 tttgagatta aaataagata gttattgcta atttttttcg taatcacaac tccatgccct
      421 ttgcttacat tttctccgcg ctcccgatag ccatcgggat tgtggtttcg gctgttttga
      481 tttatgcgtt ataatccctt aaaatggcgt gattattgct tattaactaa ttacctatgc
      541 ttaaaattca cacattaata ctttggctaa tagtatcaat tagcatcaag gcgcaaattt
      601 ctttggaagg tttatttgat gaaccaaaac aaactgccga cacgctttat tttccgtttg
      661 aagatgggat tcaattaatt gtaattaatt attatgatac atgggagcgg caggttgctt
      721 ttaaggaagc tatgcaaccc aggggattat cgtgggcagc aattatgggt ccggctccta
      781 aaaatatgta tatatttaga aataccgagg gaaagattgt taaaacttat aacagtgttt
      841 cggatggaaa aggattaaaa ggtatggact atgataacac cggtatattt ttgctgcaag
      901 gactaagaga ttttaccgag tatagcaggc atttaaaata ttatgacctg aatgaccaag
      961 taggattaat taatacgcgg ggagatattg ttgttccggc tgtatatgat gatattagaa
     1021 gatatcagga ttttgacggg gagtgggaaa aattgataat cgaaaaggat ggttattttg
     1081 gatttttgga tagtaattta aatgtattat ttccacctat ttacagatgc agcagcgaca
     1141 ccaattatta tggctacccg gagcataata ttttaaacgg gcaatatata aaagtattta
     1201 aggaagggaa atgcggttta ataaatgatc atggagatgt gttaattgat tttaaatatg
     1261 ataatttatt gcttattcat gattctatga tagttgggtt actcaacaag aaattaatta
     1321 atccaaatca atatttttgg aataatgtgc aggcctgtga aatatatgat gataaattta
     1381 acctgattac ttcactcgat gaatacgacc agattgaata caatgggatt aaaacattta
     1441 ttgtcaaaaa ggacaatctg gtaggtgtgg tgaatcacaa aggtaaagtg ataattccta
     1501 tcgaatacaa cagtttagct cctcaagata ataattacta tgtagttaaa gggaataaaa
     1561 gtggtgtgat aagtttggag ggaaaagtat taattcccat cgaatacgaa tgtaatatgt
     1621 atttctacga acctgctata tacgtcacac aagatggatt gattggtgtg tatagcgata
     1681 attatcaatt aattgctgag ccgcagtaca aacaacgcta ttgggaaatg ggcaaattca
     1741 tgctcatcag gccggacggg tcaatagggt ttgtattaca ccaaaaagga ggttcatatt
     1801 accaatcgcc ggaaggggag gtgattaaat ttgattgaat gaaagctaac gcggttgtgg
     1861 ttaaggctat ttttcgaaaa atggatatat ttatgtaatt ataaaactta aacctgaaaa
     1921 tgggttataa tgataaattc aactttattt aaatgaaaat agccggatat gtctttatac
     1981 tcttaagttt cgctgcttgt aataataaaa acacttcagt taaatcagca gctaatattg
     2041 attcgttaaa tgacgaaaat attgataatg tatctcgtga tagccctgaa tcatatcttc
     2101 atactgaaaa aaagtttacc gattcatccg ggttaaatat tactattcaa aatagttttc
     2161 caaagggtgg tccattttat agcaacaccg gatcccagtt tgggtgtgcg gtattctggt
     2221 cccggtttat taatgaaagt acaaaaccgg ttgaattaac tatcaatttt cccgctgatt
     2281 catttatagt ttttaagtca actgatgcct atttaaaatt attaatacca agtgatacga
     2341 tgtccttaga aaaagtgggg ctatataatt atggtgctac cggattgcag acatttttag
     2401 aagcaaattt tcataaagcc actggtttgc accaaattat accatctgga gatgatttta
     2461 ttttttatac gact
//
LOCUS       DAORKW010000544         2470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2536_length_2470_cov_4.330849, whole genome shotgun sequence.
ACCESSION   DAORKW010000544 DAORKW010000000
VERSION     DAORKW010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2536_length_2470_cov_4.330849"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..631
                     /locus_tag="PLB46_15285"
     CDS             335..631
                     /locus_tag="PLB46_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77941.1"
                     /translation="MINIFTYLDDFNLEHIKNTEIASSLDFGVNIGFISGKMPIEMYW
                     DIAHLYDSILPFSNTFPMSNAIGIVDCGAGEPGSINCLSAFDYEPLIMTNQPNP"
     gene            complement(1515..>2470)
                     /locus_tag="PLB46_15290"
     CDS             complement(1515..>2470)
                     /locus_tag="PLB46_15290"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRF77942.1"
                     /translation="STGSYDAATGHAAGSVNDNSTFCTYLGYDADQPDGTDISNSTAV
                     GSASRITASNQVRVGASTVTSIGGFVNWTNVSDGRFKKNVQENVPGLEFINALQPVTY
                     TLDVNGINHFLKDESNSVNEKSKIIYSGFIAQDVAMVAKNIGYDFSGVDQPENENSLY
                     GLRYAEFVVPLVKAVQELDAADQNNDSLIQTQQKQIVTLSQKITELEKLIAAQLTTNT
                     DGIIRITLEETNSPALLGQNIPNPFENSTVIPFRIPKNCNDASIVIAETSTGRIVRAI
                     PVTCNVTQLSIEAGLLSTGNYAYSLYVDGEVIGTRQMVVVE"
BASE COUNT          770 a          436 c          413 g          851 t
ORIGIN      
        1 cctaacatta actccggaca cactcgcccg taaacaattc gattttttgt ataatgttaa
       61 atttgaggat tgattttagt aaaatttgat atcattattt aacttcacct tatgaacatc
      121 tctttattaa ttgccattca aacaattgta ttatctgcga ctgcacaagc tgagccacat
      181 aggtaaatgc caatttggtt tggagacgta aatggtgaaa ttgacacagt ttgccttggt
      241 tataatcctt cttcaggtgt gaccaaaaca cttaattcag attttgaaca aaagtatgga
      301 ttgatataaa tatatttaat gttgccttgt atcgatgatc aacattttca cctatttgga
      361 tgatttcaat ttagaacaca taaaaaatac ggaaattgcc agtagtcttg acttcggagt
      421 gaacataggt tttataagcg gtaaaatgcc aatagagatg tattgggata tagcgcattt
      481 atacgattct attctaccct tttcaaatac ctttccaatg tcaaatgcaa ttggtattgt
      541 tgactgtggt gctggagagc cgggttcaat taattgtcta tctgcatttg attacgaacc
      601 attaataatg actaaccaac ctaatccgta atatatttac cacattatca gcccacatct
      661 aggggattcg cattcgtttg atgaaccgaa tgaagtgatt aatcaattta agtttttatt
      721 ttaagcatat aataactata caagtatttc ttctagtagc aatgatatta aagttcattg
      781 ttggccaaat cccattcttg atgaattaaa tatttcaagt aataatctta tttagacttt
      841 ttaaaactta aatttattcg gactacctgt aaaatctttt tccgttaaca acaaatttgg
      901 tcaatttaca tgtttggata tacgcctggg tttaattaac attcccttaa aacagctgaa
      961 aaaatattcg tattcaataa taaaaacata agctgatatt ttctgaaaaa aatcatcaag
     1021 ctattgcaaa aaaaaaatgt ttaggtaacg gcattttggg tatcggttta aaatgaatat
     1081 atattaacga cgcataaagt taggcgtgta taaattgcct agaactcatc ttaaaaatac
     1141 ctttggtgcg tcttcgctct ttatccttcg ttgcttgaat tgcccgtact ccctccatta
     1201 tgggtaattc aagcgccttg gctgacgagc gaaaacttga aattactaac acgaacctat
     1261 ttttgagatg agttctaata atttataaca agttttaata caggaacatt ttgacattat
     1321 aacatggcta cggtcatgga atacgcacaa tatctaaagg gcacttaaaa agtgtctgtt
     1381 tataacaaga gaacatcaat atccaggcaa aagtaatgga tttaattttt attataattg
     1441 actttgaaaa atgatttatt ttgatacatt tgataataaa gttgagtaaa cacagcttgt
     1501 aattcagaat aggtttattc cacaaccacc atttgtctcg ttccaataac ctcgccatct
     1561 acatacaagg agtaagcata attgccggtg cttagcaaac ctgcttcaat gcttaattgc
     1621 gttacattgc aggtaacggg aatggctctt acaattctgc ctgtggatgt ttcggcaatt
     1681 acgatgctgg catcattgca gtttttcgga atgcggaatg gtattacagt tgaattttca
     1741 aatggattgg gaatattttg acctaatagg gcaggtgagt ttgtttcttc aagcgtaatt
     1801 cttataatac cgtctgtgtt ggtggtaagt tgtgcggcaa ttaatttctc taattcagta
     1861 attttttgtg ataatgttac aatttgcttt tgttgggttt gaattaaact atcattgttt
     1921 tgatctgcag catctaattc ctgcacggct ttaaccaatg gcacaacaaa ttcggcataa
     1981 cgtaaaccat ataatgaatt ttcattttca ggttggtcta cccccgaaaa atcataacca
     2041 atatttttcg caaccattgc tacatcttgc gcaataaaac ccgaataaat gattttcgat
     2101 ttttcattta ctgaattact ttcatctttc aaaaaatggt tgataccatt tacatccaat
     2161 gtataagtaa caggttgaag tgcattaata aattcaagtc caggtacatt ttcctgcaca
     2221 ttttttttaa atctgccatc acttacattt gtccagttta caaatccgcc tatactggta
     2281 acagttgatg caccaacgcg tacctggttg cttgctgtaa ttctgctcgc acttccaact
     2341 gcggtactat ttgaaatatc tgttccatcg ggctggtcag catcgtaacc aaggtaagtg
     2401 caaaaagtgc tattgtcgtt cacgctgcct gctgcatgac cggttgctgc gtcatatgaa
     2461 cctgttgact
//
LOCUS       DAORKW010000545         2461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2550_length_2461_cov_3.730673, whole genome shotgun sequence.
ACCESSION   DAORKW010000545 DAORKW010000000
VERSION     DAORKW010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2550_length_2461_cov_3.730673"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="PLB46_15295"
     CDS             <1..1363
                     /locus_tag="PLB46_15295"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HRF77943.1"
                     /translation="DCITCAQKLGERVGNELQIPVYLYESAATQPERKNLANVRAGEY
                     EGIEQKLQQPEWQPDFGKAVFNAKSGNCIIGARDFLVAFNVNLNTTSVRRANSIAFDV
                     RENGRVDPSGQKDANGEPLRIPGTCKSVKAIGWFIEEYGIAQISMNLTNINVTPVHIA
                     FNEVNKSATKRGIRVTGSELVGLIPLQCMLDAGKYFLEQQQRSIGVSDEELIRIAIKS
                     MGLDELADFNPEEKIVEYKLYGKERFGKLISMNLRAFANETASESPAPGGGSIAAYVG
                     SLGISLATMVANLSSHKKGWDDRWKEFSDYAEKGQQLKDQLLKMVDEDTNAFNQIMAA
                     FGLPKSNEEEKNIRKKAIQDATLNAINIPYKVMELSLASMEIIKAMASIGNPNSVTDA
                     GVGALCARTAVIGAFLNVKINAGGLSDKAAAAQKIADGELLVAQAENLEKEILAIVNS
                     KIK"
     gene            1365..2273
                     /locus_tag="PLB46_15300"
     CDS             1365..2273
                     /locus_tag="PLB46_15300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRF77944.1"
                     /translation="MLIYWYVILTLFFPLLALFNLVKQQSANTYNWWSSVFLLILIVL
                     FIYQAGAWPYIGFNWRYIIVLVLGIFVLLIFRKRIKSKTQITKSKQNALSAVITIFIL
                     LFTYTNIEVVLGGYNKEQAIDLTFPLQNGSYAVMQGGDSEIGNAVHRYQTPDSYALDI
                     VKLNEHKRRGTKLFSKHIADYTIYGDTIYSPCDGTIITYRDGVDENIPPTTNSKVRGG
                     NHIVIKNGEVKILICHMQKNSIQPQKGDTVKTGEVIGLVGNTGFSIEPHLHIQAYQTF
                     NGVNKMVPIRFNGRFLNMNDIVYSDK"
BASE COUNT          822 a          416 c          497 g          726 t
ORIGIN      
        1 ggattgcatt acctgtgctc aaaaactggg tgaacgcgtc ggcaacgaat tgcaaattcc
       61 tgtttactta tatgaaagcg cagcaactca acctgaacgt aaaaacttag caaatgtccg
      121 cgccggcgaa tatgaaggca tagaacaaaa attacaacaa ccggaatggc aacccgattt
      181 cggaaaagcg gtttttaatg ctaaatccgg aaactgcatt attggtgccc gcgatttttt
      241 ggtggctttt aatgtaaatc ttaatactac cagcgtacgt cgtgctaact ccatcgcatt
      301 tgatgtgcgc gaaaatggtc gtgttgaccc atcaggacaa aaagatgcaa atggtgaacc
      361 attacgaatt cccggaacct gcaaaagtgt aaaagctatt ggatggttta ttgaagaata
      421 tggtattgct caaatttcga tgaacctgac gaatataaat gttacacctg tgcatattgc
      481 atttaatgaa gtaaataaaa gcgcaacaaa aagaggtatc cgcgttaccg gtagcgaact
      541 ggtgggatta attccattac aatgtatgct cgatgcgggc aaatattttt tagaacaaca
      601 acaacgcagc attggtgtta gtgatgaaga actcattcgc atcgctatta aaagtatggg
      661 attggacgaa ctggcggatt ttaatcctga agaaaaaatt gttgaatata aattatatgg
      721 caaggaacga tttggcaaat taatttcaat gaatttgcgt gcgtttgcca atgaaacagc
      781 aagcgaaagc cctgctccgg gtggtggcag tatagcagct tacgtaggtt cgcttggaat
      841 ttcactcgca actatggtcg ccaatctttc atcacataaa aaaggatggg atgatcgttg
      901 gaaagaattt tccgactatg ctgaaaaagg tcaacaatta aaagaccagt tgctgaaaat
      961 ggtggatgaa gatacgaatg catttaatca gattatggca gcatttggtt tgccaaaatc
     1021 taatgaggaa gaaaaaaata ttcgcaaaaa agccattcag gatgctactt taaatgccat
     1081 taatattccc tataaagtaa tggaattgag tttggcaagt atggaaatta ttaaagccat
     1141 ggcatcaata ggcaacccga attcggttac cgatgcaggt gttggagcac tatgcgcccg
     1201 cacagcagtt attggtgcat ttttaaatgt gaaaataaat gccggtggat taagcgataa
     1261 agctgctgct gctcaaaaaa ttgcggatgg cgaattattg gtagcacaag ctgaaaattt
     1321 ggaaaaagaa atattggcca ttgtgaatag caaaataaaa taacatgctc atttattggt
     1381 atgtcatttt aactttattt ttccctttac ttgctttatt taatcttgta aaacaacaat
     1441 cagcgaatac ctataattgg tggagttcag tttttctgct catattgatt gtgctattta
     1501 tttatcaagc gggtgcctgg ccatatatcg gttttaactg gagatatatt atcgttttag
     1561 tattaggcat tttcgtttta ctgatattca gaaaaaggat aaaatccaaa acccagatta
     1621 caaaatctaa gcaaaacgca ctatccgcag taattaccat ttttatatta ttattcacct
     1681 acacgaatat tgaagtagtg ttgggtggat ataataaaga acaggcaatt gatttaacat
     1741 ttccgcttca aaatgggtcc tatgccgtaa tgcagggagg tgattcggaa attggcaatg
     1801 cagttcatcg ttatcagaca cctgattctt atgcgttaga tattgtgaaa ttaaatgaac
     1861 ataaacgcag aggcactaaa ctattttcaa aacatattgc ggattatacc atttatggcg
     1921 ataccattta cagtccatgc gacggcacta taattaccta tcgagatggg gttgatgaga
     1981 atattccacc aacaaccaat tctaaagttc gaggcggaaa tcatattgta ataaaaaatg
     2041 gtgaagtgaa aatattaatt tgccatatgc aaaaaaattc tatccaaccg caaaaaggtg
     2101 ataccgttaa aacaggtgag gttattggat tagtcggcaa taccggattc agtatcgaac
     2161 cccatttaca tattcaggct tatcaaacgt ttaatggtgt aaataaaatg gtgcctatcc
     2221 gatttaatgg caggttctta aacatgaacg atattgttta ttcggataaa tgattcgctt
     2281 gtcacaaata aattagttta ggatttatca cttataggtc caatacaacg gcttagttct
     2341 ttttcaaaac agcgccaaaa agtgtatccc caccttccga agaatattgg aaatattttt
     2401 gttcaattaa ttgcaatggt aaatgttgag tgaaataagc gacattgttt tcattttcat
     2461 c
//
LOCUS       DAORKW010000546         2454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2560_length_2454_cov_4.147978, whole genome shotgun sequence.
ACCESSION   DAORKW010000546 DAORKW010000000
VERSION     DAORKW010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2560_length_2454_cov_4.147978"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLB46_15305"
     CDS             complement(<1..828)
                     /locus_tag="PLB46_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRF77945.1"
                     /translation="MDAKFSPKVKEVISYSREEALRLGHDHIGTEHLLLGLIRDGDGL
                     AIKVLMSLDVDIAVLRKTVEDAIKDKTSRQPFNAGSLPLTKQAEKVLKITVLEAKVLK
                     SDTIGTEHLMLSILKNKDNIATQILGQFDVDYDVFRQELDYVRQDKGLKDTPPTNQQE
                     DDGFEEEDKGDRGFNVPRKPGNAKSKTPVLDNFGRDVTRAAEEGRLDPIVGRQEEIER
                     VSQILSRRKKNNPILIGEPGVGKTAIVEGLALRIIQRKVSRVLFDKRIIMLDLAALVA
                     "
     gene            1049..1420
                     /locus_tag="PLB46_15310"
     CDS             1049..1420
                     /locus_tag="PLB46_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRF77946.1"
                     /translation="MKFTVDKKERYCIFKLDEPKLDSIISANLKSELVILNQEGFRNI
                     ICDLSDVNFIDSSGLSALLIGHRLCKESNGTFVLTGCGDSTVKLIKISQLDTILNVVP
                     TESEATDFILMEEIEREIGEE"
     gene            1424..2344
                     /locus_tag="PLB46_15315"
     CDS             1424..2344
                     /locus_tag="PLB46_15315"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRF77947.1"
                     /translation="MSFSVTVIGSNSAIPAHGRHPSAQVVNINDKLYLIDCGEGTQMR
                     FNELGIKWMKINHIFISHLHGDHYFGLIGVVSTYHLLKRNRPLYIYGPQALLDILQLQ
                     LHVGQTDLCYELVFRALDPTRSETIFDGEDITIDTIVLKHRIDCVGFLFREKQRERKI
                     NREKFHELDIPVELIPEIKKGKDVLDEKTGERILNKDITLDPLRARAYAYCCDTAFNP
                     EMASQIKGVDLLYHDCTFDAAGEQRAADTFHSTTIQAAQMASLAAVNKLMIGHFSSKY
                     DDLRPLLEEAKTIFENSILAIEGKVVVVER"
BASE COUNT          710 a          480 c          509 g          755 t
ORIGIN      
        1 tgcaaccaat gctgcaaggt cgagcatgat aatacgtttg tcgaataaca cacgagatac
       61 cttacgctga ataatgcgca gtgctaaacc ttccacaatt gcagttttac ccacaccagg
      121 ttcaccaatt aaaatcggat tatttttttt gcgtcggctt aagatttgag aaacacgttc
      181 aatttcttcc tgacggccaa caattgggtc gagacgacct tcttcggcag cgcgtgttac
      241 atcgcggcca aaattatcga gcacaggtgt ttttgatttt gcgttacccg gtttgcgtgg
      301 cacattaaag ccacgatcgc ccttatcctc ttcttcaaaa ccatcatctt cctgttggtt
      361 ggttggagga gtatctttta aacctttgtc ctgacgcaca taatcgagtt cctgacggaa
      421 tacgtcatag tccacgtcaa attgtcctaa aatctgtgtt gcgatgttat ccttgttttt
      481 gaggatagac aacatgaggt gttcagtgcc aattgtgtcg cttttgagca ctttggcttc
      541 gagcacagtt attttgagca ccttttcagc ttgtttggta agcggcaaac tgcctgcatt
      601 aaagggctgg cgtgaggttt tgtcctttat ggcatcctcc acggttttgc gcaacacggc
      661 gatatctacg tcgaggctca tcagcacttt tatggcaaga ccgtcgccat cacgtatcag
      721 tccgagcagt aaatgttcgg taccaatatg gtcgtgtccc agtcgcagcg cctcttcacg
      781 gctgtaagag atcacttctt ttacttttgg agaaaatttt gcgtccatta tatctttctt
      841 tcaaattttg aaccatccct gcaatcctga caaaataaca aggaaagttg tgataaagtt
      901 gtgacttgat tgtcgtttta gtgtaaacgt tacagtccaa ataaaattcc ctttcccggt
      961 gttttctaat tgactaacaa atttggaaaa aattttaatt acttaccttc gcagagtaaa
     1021 gtttattttt acacctaata acaacagtat gaagtttaca gtagacaaaa aagagcgtta
     1081 ttgcattttt aagctagatg agcctaaatt ggactccatt atatctgcca acctgaaaag
     1141 tgagcttgta atccttaacc aggaagggtt tcgcaacatt atttgcgact taagtgacgt
     1201 taattttatc gattcttcgg gtttgagtgc ccttttaatt ggtcaccgcc tttgtaagga
     1261 gtcgaacggc acctttgtgt tgaccggctg tggtgattct accgtgaagt tgataaagat
     1321 atcgcaattg gataccattt tgaatgtggt gcctactgaa agtgaggcaa ctgacttcat
     1381 tttaatggaa gaaattgaac gcgaaatagg cgaagagtaa gcaatgtcgt tttcggttac
     1441 cgttatcggg agcaatagtg ccatacctgc gcatgggcga cacccaagtg ctcaggtggt
     1501 aaatatcaat gataaattat atttaattga ttgtggtgaa ggcactcaga tgcggtttaa
     1561 cgagctgggg attaagtgga tgaaaatcaa tcatattttt atctcccact tacatggcga
     1621 ccattatttc ggattaattg gtgtagtttc cacttatcat ttattgaaac gcaaccggcc
     1681 attatatatt tatgggccgc aagcattact tgatattctg caattacaat tgcatgttgg
     1741 gcaaactgat ttatgttatg aattagtatt tcgcgcactt gacccaacac gttcggaaac
     1801 aatttttgat ggtgaagata ttaccattga taccattgtt ttaaaacacc gaatagactg
     1861 tgtaggattt ttatttcggg aaaaacagcg ggaaagaaaa ataaatcgtg aaaagtttca
     1921 cgagttggat attcctgttg aattaattcc ggagataaaa aaaggtaaag atgttctgga
     1981 tgaaaaaacc ggtgagcgga ttttaaataa agacattact ttggatccgc tgcgcgcacg
     2041 tgcttatgcg tattgctgcg acacggcatt taatcctgaa atggcatcac aaattaaagg
     2101 ggttgattta ttataccacg attgcacttt tgatgcagcc ggagagcaac gcgctgcaga
     2161 tacttttcat tccacaacca tacaagcagc acaaatggca tcgcttgctg cagtaaataa
     2221 attaatgata ggccattttt cttctaagta tgatgattta cgaccattgc ttgaagaagc
     2281 aaaaacgata tttgaaaata gtattcttgc tattgaaggg aaggttgttg tggtggagag
     2341 gtgaaaaaaa aaatcccgca cctttcagtg caggattaaa tactttcgaa aactatatta
     2401 caacaacgca ctcatcatcc aaccaataat taacgccata attcccacaa taat
//
LOCUS       DAORKW010000547         2453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2562_length_2453_cov_4.263136, whole genome shotgun sequence.
ACCESSION   DAORKW010000547 DAORKW010000000
VERSION     DAORKW010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2562_length_2453_cov_4.263136"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PLB46_15320"
     CDS             <1..874
                     /locus_tag="PLB46_15320"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77948.1"
                     /translation="SVEDIDINMVNDNVASGISVLDVAVPSVEEGFVGATYPPANWAV
                     DNLNDGTGWSRATGAGGYGESTTSTKADFYNIAVGTFDLYLPKLDFTNFTGDLNLVFD
                     RAYAMYNATFVDILQVQVSEDCGATWDELYEKSGSELNTAPNTTSLFKPDDDEWETDE
                     LDMSAYAGNADVLVRFHAISGYGNNLYLDNIRIAGPTGVFNIPTLNQFELYPNPTSTS
                     SVASFNLIEAADVTINVIDVTGKVVAAINAGNMISGSNTVTIETADFAAGMYNVVISA
                     NGKNVAFENLVVIK"
     gene            1042..2328
                     /locus_tag="PLB46_15325"
     CDS             1042..2328
                     /locus_tag="PLB46_15325"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77949.1"
                     /translation="MKTLFLSLFFVVITHVAFGQVTPYTTAVTYPLILAFPMDVSSCG
                     SVAFNPIDIRYYTSRVGNATYPLLTFYADGTLAFQDVTGQDTRGIWWNPNTNQLERNC
                     FSAVGWSQIVLDGSKNATSAFNILFAGALNPNAQCEGAYDYINNEVVFYFGGNISRYN
                     RATGTLIATTAITGVTFTSINTTAVIYTGVTGYEYGILDYTAKKVIFLNRATCAYAGE
                     TALPATAVTATSHQFSYTNNLLWLFNTTDNKWYSYQVFDLPVLAIDDIILNGVAEPTQ
                     NTLTWQAISDAPITVFNVLHSSDGVHFKQIGSVNGYDSGDGLYSYTHMHPTNGFNYYR
                     IQCADDQQVEFLSEIICIEAKTGKNNITLYPNPTTTYIIINEEIDQRTYQILNSSKQI
                     IKQGELDENGKIDVHSLEAGIYYLQMDEKSYPFVKK"
BASE COUNT          750 a          399 c          468 g          836 t
ORIGIN      
        1 ttcagttgaa gatattgata tcaatatggt taacgacaac gttgccagtg gtattagtgt
       61 tttagatgtt gcagttccta gcgttgagga aggttttgtt ggagctactt accctcctgc
      121 aaactgggca gttgataact taaacgacgg aacaggctgg agccgtgcaa ctggtgccgg
      181 tggttatggt gaaagtacta cttctacaaa agctgatttt tataacatcg ctgttggaac
      241 attcgatttg tatttaccta aattggattt taccaatttt accggtgatt taaatttggt
      301 atttgatcgt gcttatgcaa tgtataatgc tacatttgta gatatacttc aagtacaggt
      361 ttctgaagat tgcggtgcaa cttgggatga attatatgaa aaatctggtt ctgaattaaa
      421 tacagctcct aatacaactt cattattcaa accggatgat gatgaatggg aaactgacga
      481 attagatatg tctgcttatg caggtaatgc tgatgtattg gtgagattcc atgctatcag
      541 cggttacggc aataacttat atttagacaa tattcgtatt gcaggcccaa caggtgtatt
      601 taatattcct actttaaatc aattcgaatt atatccaaac ccaacttcaa ccagctctgt
      661 agcttcattt aatttaattg aagctgctga tgtaactatc aatgtaattg atgttaccgg
      721 taaagttgtt gctgctatca acgcaggaaa tatgatttca ggtagtaata ctgttactat
      781 tgaaactgct gattttgcag caggtatgta caatgtagtt atcagtgcaa acggtaaaaa
      841 cgttgcattt gaaaacttag ttgtaattaa ataataaatc aagcaacaag taaacataga
      901 ggtcgtcctt ttgggcggcc tctttttttt gtacccccag aattgggtat tgtaaaaagt
      961 gccttctgaa cggagttttg ttccatcaaa atcaccttta accggtgata ttttttaact
     1021 gatgtaacaa accttttatt tatgaaaaca ctttttttaa gtttattttt tgtggtaatt
     1081 acgcatgttg cttttggaca ggttacgccc tataccacag cggtcactta tcctttaatc
     1141 cttgcatttc caatggatgt ttccagttgc gggtcggtag catttaatcc tatagatatc
     1201 agatattata cttctcgtgt cggcaatgct acttatccat tacttacttt ttatgcagat
     1261 ggcactttag cttttcagga tgtaaccgga caggatacca gaggtatttg gtggaatccc
     1321 aataccaacc aattagaacg caattgtttt tcagctgtag gctggtctca aattgtttta
     1381 gatggcagca agaatgccac cagtgcgttt aatattttat ttgcaggcgc attaaaccct
     1441 aatgcacaat gtgaaggtgc atacgattat atcaataatg aagttgtatt ttattttgga
     1501 ggtaatattt cacgatacaa cagagcaacc ggaacgctaa tagccacaac tgcaattacc
     1561 ggtgttactt ttacttcgat taacaccaca gctgtgattt atacaggtgt aaccggttat
     1621 gaatatggta tattggatta tactgccaaa aaagtaattt tcctcaatcg tgcaacttgt
     1681 gcatatgctg gtgaaacagc attacctgca acagcagtta ctgccacatc acatcaattt
     1741 agttatacca ataatttgtt gtggttattt aatacaaccg ataataaatg gtatagttat
     1801 caggtatttg atttacctgt tttagctatt gatgatatta ttttaaatgg tgtggcagag
     1861 cctacacaaa acacgcttac atggcaagct atcagcgatg caccaataac agtatttaat
     1921 gttctgcata gcagcgatgg ggtgcatttt aagcaaattg gcagtgtgaa tggctatgat
     1981 tccggagatg gtttatattc ctatacgcat atgcatccta ctaatggttt taattattat
     2041 cgtatacaat gtgcagatga tcaacaggta gaatttttat ctgaaataat ttgtattgaa
     2101 gcaaaaacag gtaaaaataa tattaccctt tatcctaatc cgactactac ctatattatt
     2161 attaatgagg aaattgatca gcgcacctat caaatattaa atagttcaaa acaaataatt
     2221 aaacaagggg agctcgatga aaatggtaaa atagatgtgc attccttaga agcaggtatt
     2281 tattatttgc aaatggatga gaagagttat ccttttgtga aaaagtaaat taagtggttt
     2341 tgttacagtt aaggtgctca tttgggcact ttaatttttt tattgttgta acaattttca
     2401 acagttttta gccataaaaa aaaccagcag tagctggttt tttattgttg ttt
//
LOCUS       DAORKW010000548         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2568_length_2449_cov_4.573099, whole genome shotgun sequence.
ACCESSION   DAORKW010000548 DAORKW010000000
VERSION     DAORKW010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2568_length_2449_cov_4.573099"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLB46_15330"
     CDS             complement(<1..712)
                     /locus_tag="PLB46_15330"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRF77950.1"
                     /translation="MSNESEIVALIALLDDSDQEVFDHVAARLIALGPMVIDKLEDAY
                     TSIPNPVMQERIEELIHHIQFTRVEHDLKIWAEHENNDLLKGVLIITRHQYPELDEEK
                     LLKTISRIQKDIWIGINNYLSPLEQMNVVNQTLFSHYQFLGLNNDDDELRYMYINNAV
                     DALKGNHFAIGILYLCLCQQLDLPVYGVCLSAHFILARAKDYITDFDNKEENREEVLF
                     YVNPYNKGLAFSEKEINIY"
     gene            complement(746..>2449)
                     /locus_tag="PLB46_15335"
     CDS             complement(746..>2449)
                     /locus_tag="PLB46_15335"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRF77951.1"
                     /translation="KLGQEGDAEKFVNDCNGAKFAVSNIAVKPAKRTPSAPFTTSTLQ
                     QEASTKLGFSVARTMMIAQKLYEEGHITYMRTDSVNLGDQAMDAIKSEIVKNYGDKYA
                     NPRKFKTKSAGAQEAHEAIRPTYMENHEVDADFDEKRLYKLIWKRTMASQMADAELEK
                     TVVDIAVSTRKENLAAKGEIIKFDGFLKVYTEALEDVKDEDATLLPPLKIGQDLALVE
                     MIGAQKFSKPGARYTEASLVKKLEELGIGRPSTYAPTISTIQKREYVEKKDKEGVERT
                     FIQYTLKDGKVQKQTLTETFGAERMKLFPTDLGILVTDFLITNFPQILDYNFTANVEE
                     QFDEIAVGKIDWHKMIDDFYKPFHAHLEVTTKTAQKITGERYLGLHPNGEPLKVMMGK
                     YGAMVVMGETLDPKELKNNPDAKKPKFAGLLKTQNMAELTLEDAIKLFELPKTIGTYR
                     EDDVVVGIGRFGPYVRNQKKFYSIPKGTEAASITLEEAIELILAKDEKDANKLIHDFP
                     EHEVQVLNGQYGAYIKKGKENYKIPKDKVAKDLTLDDVLDIIKNTDPSVKGKKRFAKK
                     K"
BASE COUNT          679 a          501 c          392 g          877 t
ORIGIN      
        1 aataaatatt gatttctttt tcgctaaatg ccaaaccctt gttataagga tttacataaa
       61 acaaaacctc ttccctattt tcttctttat tatcgaaatc ggtaatataa tcttttgcac
      121 gagctaaaat aaaatgcgca ctcaaacaaa cgccatatac cggcaaatca agttgctggc
      181 ataaacacaa atacaaaatg ccaatggcaa agtgatttcc ttttagtgcg tcaacagcat
      241 tattaatata catgtaacgc aattcatcgt catcgttatt gagccctaaa aactgatagt
      301 gtgaaaataa agtttgattt accacattca tctgctcgag tggggacaaa taattattaa
      361 taccaatcca gatatctttt tgaatacgtg aaatggtttt taataatttt tcttcatcta
      421 attcaggata ttggtgtcgg gtaataatta aaacaccttt gagcaaatcg ttattttcat
      481 gttccgccca aattttcaaa tcgtgctcaa cgcgtgtaaa ctggatatga tgaatgagct
      541 cctctatacg ttcctgcatt acagggttgg ggatagaagt ataggcatct tccaatttat
      601 caattaccat gggacctaag gcaattaatc gcgcagcaac gtggtcaaat acttcctgat
      661 cactgtcatc taacaatgca attagcgcga ctatttcact ttcgttgctc actggtattt
      721 tatttactca actgcctgac taaaattatt tctttttcgc aaaacgtttt ttacctttta
      781 cggaaggatc ggtatttttt ataatatcca aaacatcatc aagggttaaa tctttagcca
      841 ctttatcttt tggaatttta taattttctt ttcccttttt aatatatgca ccgtattgtc
      901 cgttcagcac ctgcacttca tgttctggaa aatcgtgaat cagtttattt gcatcttttt
      961 catctttcgc caaaattaat tcaatggcct cttctaaagt gatgcttgct gcttccgttc
     1021 cttttggtat agaataaaac tttttctgat tgcgcacata aggtccgaaa cgaccaatac
     1081 caacaactac atcatcttca cgataagtac caattgtttt tggtaattca aataatttaa
     1141 tggcatcttc caaagttaat tctgccatgt tttgtgtttt gagtagaccg gcaaattttg
     1201 gttttttggc gtcgggatta ttttttaatt ctttcggatc cagcgtctct cccattacca
     1261 ccatggcacc gtattttccc atcatcactt tgagtggttc accatttgga tgtaagccca
     1321 aataccgctc tcctgttatc ttttgagctg ttttggtagt tacttccaaa tgcgcatgaa
     1381 atggtttgta aaaatcatca atcattttat gccagtcgat tttaccaacg gcaatttcat
     1441 caaattgttc ttccacattt gcagtgaaat tataatcgag aatttgcggg aaattggtaa
     1501 ttaaaaaatc ggtgactaaa atgcctaaat cggtaggaaa taatttcata cgttcagcac
     1561 caaatgtttc ggtaagtgtt tgtttttgca ctttaccgtc ttttaaggta tactgaataa
     1621 acgtacgttc cacaccttct ttatcttttt tctctacata ttctcttttc tgaatagtag
     1681 aaattgttgg tgcataagta gatggtcgac caatgcctaa ttcttccaat tttttcacca
     1741 gacttgcttc agtgtatcgc gcaccgggtt ttgaaaattt ttgtgcgcca atcatttcaa
     1801 ccaacgctaa atcctgacca atttttaatg gtggtaataa ggtagcatct tcatctttaa
     1861 catcttctaa tgcttcagta tatactttta aaaatccgtc gaattttatg atttcacctt
     1921 tcgcagccaa attttcttta cgggtagaaa cggcgatatc cactaccgtt ttttccaact
     1981 ctgcatccgc catttgcgat gccatggtac gtttccaaat aagtttatac aaacgttttt
     2041 catcaaaatc ggcatctact tcatggtttt ccatatatgt agggcgaatg gcttcgtggg
     2101 cctcttgtgc acctgccgat ttggttttaa acttgcgcgg attagcatat ttatcgccgt
     2161 aattttttac aatttcggac ttaatagcat ccattgcctg atcacctaaa ttcactgagt
     2221 ctgtcctcat ataggtgata tgcccttctt catataattt ctgggcaatc atcattgttc
     2281 ttgccactga aaatcccaat ttagtggaag cttcctgttg taatgttgaa gtagtgaatg
     2341 gtgctgaagg cgttcttttt gccggtttaa ccgcaatatt agatactgcg aattttgcac
     2401 cgttacaatc gttgacaaat ttctccgcat ccccttcctg tcctaattt
//
LOCUS       DAORKW010000549         2445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2573_length_2445_cov_4.328033, whole genome shotgun sequence.
ACCESSION   DAORKW010000549 DAORKW010000000
VERSION     DAORKW010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2573_length_2445_cov_4.328033"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /locus_tag="PLB46_15340"
     CDS             <1..1891
                     /locus_tag="PLB46_15340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77952.1"
                     /translation="LIGIVYAAATYAQEVLRPLKYNPTLFQQQNIAGSRSIVENRDTL
                     CIPFIDDFSANLEIIDGAATDCGDTIVHTATGIFPSNLFWVDSNAFVNRTYGSLPPTY
                     GVITLDGLNKFGKPYNEASSFDMADELTSKPIYLAAPTDSVYLSFYYQPGGFGEFPNL
                     EDSLILDFQNSDGTWTRVWDATNTLGNDPQSFKLAMVPIGDSYFYDGFRFRFRNWAGV
                     NGNNDHWNIDYVLLDDERTFNDTLFRDVAIVYQPERYLKRYRQMPWNQFKNHQADELA
                     SGHGVFMYNNFNTVINTSHSYQVHEKYAGDEIIAPTSPVSINLDPFTLAYNSYANFEI
                     PEATTGFDGDSLAINFRYEISPSGDVNLRNDTLDYEQAFYNYYAYDDGTAERAFALVG
                     TGAKLAIHFYANEPDTLKEVYIHWAYVDGNKSNLFFSLVVWDDIDTTLTSADENIIFQ
                     NDFLTPKYVDSINGFYVYKLIDFLGNPTPVVVDGDFYVGWLQTQDEFLNVGFDVNNNA
                     SEQVYYNVGGAWQQSALTGAIMIRPQVGGDYSKYSQIGEQAATQHTLSIYPNPVSQTL
                     HVQTSLTGELLYQVFDQSGRLVLAENGFNNQIAVQQLSAGFYILKVNELTTGNVLVEK
                     FIKEK"
     gene            1946..2251
                     /locus_tag="PLB46_15345"
     CDS             1946..2251
                     /locus_tag="PLB46_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRF77953.1"
                     /translation="MDITVEELKQRMDAGEDLFIIDVREIHEYEEFNIGAENIPLATI
                     PMRMYDFDDDKDREIIVHCRSGVRSNTAKAILVQSGFTNVKNLIGGMLEWQSKFGSK"
     gene            2248..>2445
                     /locus_tag="PLB46_15350"
     CDS             2248..>2445
                     /locus_tag="PLB46_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HRF77954.1"
                     /translation="MTDNKQRIVFFDGVCNLCNASVQTILKNDKQQLFYFASLQSEFA
                     GQFFLQHQYQAPSSTIIYFDGE"
BASE COUNT          762 a          420 c          486 g          777 t
ORIGIN      
        1 gcttattggt atagtttatg ccgctgcgac atatgcgcaa gaagtattga ggccgttaaa
       61 atataatcct accttatttc aacaacaaaa tattgccgga agccgatcaa ttgttgaaaa
      121 ccgtgatacg ttatgtattc catttattga tgatttttct gctaatcttg aaataattga
      181 cggcgctgct actgattgtg gtgataccat tgtgcatact gcaacaggca tttttccttc
      241 taatttgttt tgggtagata gtaatgcttt tgtaaaccgc acttatggtt ccttaccacc
      301 aacttacggt gtaattacac ttgatggtct aaataaattc ggaaaaccat ataacgaagc
      361 atccagtttt gacatggcag atgaattaac ttcaaaacct atttatttag cggctccaac
      421 agattcagtc tatttgagtt tttattacca gccgggaggt ttcggcgaat ttccgaattt
      481 ggaagattca ttaattctcg attttcaaaa tagtgatggt acatggacac gtgtttggga
      541 tgctaccaat acgcttggta atgaccctca atcgtttaaa ttagcgatgg tgcctatagg
      601 ggatagttat ttttacgatg gatttcgctt tcgttttcgc aactgggcag gagtaaacgg
      661 aaataatgac cactggaata ttgattatgt gttgttagat gatgagcgca cttttaatga
      721 tactttattt cgtgatgttg ctatagttta tcagccggaa cgttatttga aaaggtatag
      781 acaaatgcca tggaatcaat ttaaaaacca tcaggctgat gaattagcct caggccatgg
      841 tgtatttatg tataataatt ttaataccgt aattaataca tcacacagtt atcaggtgca
      901 cgaaaaatat gcaggtgatg aaattattgc accaacttct cctgtctcta tcaaccttga
      961 cccatttact ttagcgtata acagttatgc taatttcgaa attcctgaag caacaacagg
     1021 ctttgatggc gatagtttag ccattaattt tcgatatgaa atttcccctt cgggagatgt
     1081 gaatttgcgc aacgacacat tggattatga gcaggcattt tataattatt atgcttatga
     1141 tgatggcacg gcagaacgtg catttgcatt agttggtaca ggcgcgaaac tagccataca
     1201 tttttatgcc aacgaaccgg acactttaaa agaagtgtat attcattggg cttatgtgga
     1261 tggcaataaa agcaacttgt ttttttcatt agttgtgtgg gatgatatag atacaacctt
     1321 aacatcggct gatgagaata ttattttcca aaacgatttc cttacaccaa aatatgtaga
     1381 ttcaataaat ggattttacg tgtataagct catcgacttc cttggtaacc ctacacctgt
     1441 tgttgttgac ggtgattttt atgtaggctg gttgcaaact caggatgaat ttttgaacgt
     1501 gggtttcgat gtaaataata atgcaagtga gcaggtatat tataatgttg gcggtgcatg
     1561 gcagcaatcg gctttaacag gtgcaattat gattcgccct caggttggtg gcgattattc
     1621 taaatatagt caaattggag agcaagcagc aacacaacac accttgagta tttatcctaa
     1681 tccggttagt caaaccttac acgtccaaac atcattaaca ggtgaattat tatatcaggt
     1741 atttgatcaa agtggcagac ttgtattggc agaaaacgga tttaataatc aaattgctgt
     1801 acaacaactc agtgcaggtt tttacatact caaagtgaac gaattgacaa caggtaatgt
     1861 tttggtagag aaatttatca aagaaaaata atagttccca ttcagggata acatttcagg
     1921 caatcacaac tcaaattaat tacaaatgga catcacagtt gaagaactca aacaacgtat
     1981 ggatgcgggt gaagatttat tcatcatcga cgtacgagaa atacatgagt atgaagaatt
     2041 taatatagga gcagaaaata taccgcttgc tactatacct atgcgcatgt acgattttga
     2101 tgacgataaa gaccgcgaaa taatagtgca ttgtcgttca ggtgtgcgca gtaatacagc
     2161 taaagccatc ctcgtgcaat ctggttttac caatgttaaa aatttgatag gaggaatgct
     2221 tgaatggcaa agtaagtttg gctcgaaatg accgacaaca aacaacgtat tgtttttttt
     2281 gatggtgttt gtaatctctg caacgccagc gtgcaaacca ttttaaaaaa tgataaacaa
     2341 cagttgtttt atttcgcatc actgcaatca gaatttgccg gtcaattttt tcttcaacac
     2401 caatatcagg cgccatcatc aaccataatt tattttgatg gtgag
//
LOCUS       DAORKW010000550         2442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2579_length_2442_cov_4.750733, whole genome shotgun sequence.
ACCESSION   DAORKW010000550 DAORKW010000000
VERSION     DAORKW010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2579_length_2442_cov_4.750733"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PLB46_15355"
     CDS             complement(<1..703)
                     /locus_tag="PLB46_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77955.1"
                     /translation="MLGKRLNKIFGLFAWCALVPALGFGQADSSYHVVTPQVGLFPKY
                     APDELYRVAVSGFYRFYGTQTRNFTPYLLSTAGGQTTPTNQLFIGDDTQLPTLQLNVS
                     GRPTLKTSWSFDIYTYQYLNGTLGSAYGQQVVDSLRPSIQNPIEGTRLGGSMILNLGI
                     NFTATHETGFGSFTLRSGGIQWHVLSDLTMGSFRGYNRFTLFERNPWDPITNSVFGRY
                     DKYCNEGAIDQDVRWG"
     gene            1387..1791
                     /locus_tag="PLB46_15360"
     CDS             1387..1791
                     /locus_tag="PLB46_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77956.1"
                     /translation="MKNLLCLILILVAFNTNVFCQVETFETWKTYENTQIELNKIIGA
                     WYATDSIESAINFVSLNETLVYIDGIESGVGGPYSFRLDQNGVFVNGSAANWPPYYCT
                     LFLREINVLEIKFWNFLGPESTSILYKRMKSD"
     gene            2239..>2442
                     /locus_tag="PLB46_15365"
     CDS             2239..>2442
                     /locus_tag="PLB46_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77957.1"
                     /translation="MKKYFSQNQIFTFLTILFLVSSCNSAIKKDLVKEKEQPKLIKTP
                     GNGNLSCGLQDKSGNLWFGTSHNG"
BASE COUNT          771 a          442 c          451 g          778 t
ORIGIN      
        1 taccccaacg tacatcctgg tcaatagcac cttcattaca atatttatca tagcgcccaa
       61 aaacactatt cgtaataggg tcccatgggt tgcgttcaaa caaggtaaaa cggttatatc
      121 ctcgaaacga gcccatggtt aagtcgctca gcacatgcca ttgtatacca cccgaacgca
      181 gtgtaaatga gccgaagccc gtttcgtgcg tagcggtaaa attgataccc aggtttaaaa
      241 tcatgctgcc gcccaagcga gttccttcaa tcgggttttg gatgctgggg cgcaaactgt
      301 ctacaacctg ctgtccgtaa gcacttccga gtgttccatt taaatattga taggtataaa
      361 tatcgaaaga ccatgatgtt tttaaggtag gtcggcccga aacattcagt tgcagggtag
      421 gtaattgcgt gtcgtcgcca ataaacagct ggttggttgg ggtagtctgg ccacccgcag
      481 tactcaataa atacggggta aaattgcgtg tttgtgtgcc ataaaaccgg taaaacccgg
      541 aaacggcaac gcggtacagt tcatccggcg catattttgg aaaaagtccc acttgcggag
      601 taaccacatg gtagctcgaa tcggcttgtc caaaccccaa agccggcacc aatgcacacc
      661 aggcaaacag tccgaatatt ttgtttaatc ttttccccaa catttatagg ttttgcgtat
      721 taaaactaca tatttatgtt tcaaatgtaa tactttttgt aaaaattaca attagtcaaa
      781 ggttttttcc tggtttttag aaagttaact gctttagctt ggatgattaa gaccgatgtc
      841 actaaaatcc atttcctcaa aaaaagaccc tggttaaggt aaatgtcagg cttgctgata
      901 accactcatc attcatttct catttctcat ccaatgagtt gttcgctggg ctcacatgag
      961 tgatgagtaa tgagtggtga ctacaacact tgaaatgatt tctttttatt tatatgttta
     1021 catagatgaa aattttcagt agaagaactt gagttgaaat ttaaactcgc ttattattcc
     1081 tcactcattt ctcattcgaa aaattagcaa acgtcatcgt gtaggggcga attgcatccg
     1141 cccatacatg atggcgttta ccgaatgttt ggataaatta aaaacattca atatcccgaa
     1201 atccaacttt aatttttcat taaaggtcga ggcgcattta cactcatcgc caattcctta
     1261 tccaatgaaa gtttcgcaag gcgagttagg gaataatcgg ctatacttaa acggatttgt
     1321 agaattgtgt atattagcaa atatattgtt attgcctata ccgttaacac tattctataa
     1381 aaatacatga aaaatttact ctgccttatt ttaattctag tcgcttttaa tactaatgta
     1441 ttttgtcaag tagaaacatt tgaaacatgg aagacatatg aaaatactca aatagaatta
     1501 aacaaaatta taggtgcttg gtacgctact gattcaatcg agtctgctat taactttgtt
     1561 agcttgaatg aaacattagt atatattgat ggaatagaga gtggagttgg tggtccttac
     1621 tcatttagac ttgatcaaaa tggtgttttt gttaatggtt cggcggcaaa ttggccccct
     1681 tattattgta ccctgttttt gcgtgaaatc aatgtacttg aaataaaatt ttggaatttt
     1741 ctaggcccgg aatctacaag tattttgtat aagagaatga aaagtgatta aaaggctact
     1801 tgcaacttat ttagaataga ttttttttgt aatagttgat ttttgtttgc taaaggatta
     1861 aacggatagg ttttaaaaat ttgtttaggt aatcacaaaa aaataactcg acttaaccct
     1921 gctgtaattt aatcactttc atttattcca atcaatgata tgcaaaatac ttaacttaga
     1981 atgctgctta aaaacaaatt gagttggcgc aaggatgttt tttgacttcc agtcaacaaa
     2041 ttatgaaatt gtggcaatta ggaggcttaa ggacaacctt tttaggtgta ttcgagcatt
     2101 attacttctt tttcggataa tttggtagtt ttgaaaagat gaagggacag ggttctttaa
     2161 ttacaattgg cagttacaag gtaatttcaa aaccacaacc ttcaaataaa taacaaacca
     2221 taaactgttg tttcaccaat gaaaaaatat ttctcccaaa accaaatatt cactttcctg
     2281 actatactat ttttagtttc atcctgcaat agcgcaatta aaaaggattt ggtcaaagaa
     2341 aaggaacaac caaaacttat taagacccct ggtaatggta atctttcttg tggactgcag
     2401 gacaaatccg gtaatctttg gtttggaacc tctcataatg gg
//
LOCUS       DAORKW010000551         2439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2586_length_2439_cov_5.026846, whole genome shotgun sequence.
ACCESSION   DAORKW010000551 DAORKW010000000
VERSION     DAORKW010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2586_length_2439_cov_5.026846"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..586)
                     /gene="rplF"
                     /locus_tag="PLB46_15370"
     CDS             complement(32..586)
                     /gene="rplF"
                     /locus_tag="PLB46_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRF77958.1"
                     /translation="MSRIGKLPIQLPAGVTVSVADNNVVTVKGKRGELTLKVDSDIAI
                     KPEGSVVKLERPTEQKRHKAMHGLYRALIANMVKGVSEGFKKELELVGVGYRAANTGQ
                     ILEIAVGYSHPIMMMIPKEVKLSTVSEKGKNPTIILESNDKQLLGAIAAKIRSFRKPE
                     PYKGKGIKYSDEVLRRKAGKTAGK"
     gene            complement(609..1010)
                     /gene="rpsH"
                     /locus_tag="PLB46_15375"
     CDS             complement(609..1010)
                     /gene="rpsH"
                     /locus_tag="PLB46_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRF77959.1"
                     /translation="MKVTDPIADFLTRIRNAQKANHRIVEVPASNMKKRLTEILYEHG
                     FIFRYKFEEEGPQGTIKIALKYDPVTKSPAIKGLTRVSKPGLRKYAGVGEMPRVLSGL
                     GLAIVSTSKGVMTEKEAKRQNVGGEVLCYVY"
     gene            complement(1035..1304)
                     /gene="rpsN"
                     /locus_tag="PLB46_15380"
     CDS             complement(1035..1304)
                     /gene="rpsN"
                     /locus_tag="PLB46_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRF77960.1"
                     /translation="MSKKSIIARNTKRQRMITHYADLRKQLKAEGKWEELDKLPRNSS
                     QVRYRNRCKLTGRPRGYISDLGISRIKLRDLALYGKIPGMTKASW"
     gene            complement(1314..1868)
                     /gene="rplE"
                     /locus_tag="PLB46_15385"
     CDS             complement(1314..1868)
                     /gene="rplE"
                     /locus_tag="PLB46_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRF77961.1"
                     /translation="METRTPRLKKKYSEEVVPSLQEKFNYKSSMQLPKLLKISINQGV
                     GKATQDKKMIDVAIDEMTRITGQKCVATVSKKDISNFKLRSAQPIGVKVTLRGENMYE
                     FLDRLFAVALPRVRDFKGVNDKSFDGRGNYTLGITEHIIFPEIDIDKVTRISGMDITF
                     VTSAKTDAEAYELLKGLGMPFRKP"
     gene            complement(1868..2212)
                     /locus_tag="PLB46_15390"
     CDS             complement(1868..2212)
                     /locus_tag="PLB46_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRF77962.1"
                     /translation="MATKNTNSRFKPKYHVKKNDTVLVITGDDKGKKGRVVEVNYTTG
                     RVMVEGVNIISKHTRPTQQYPNGGIIKKEAPIHISNVQLVDPKTGKGIRVNRKEVEGK
                     IVRTNKSGEIIK"
     gene            complement(2215..>2439)
                     /gene="rplN"
                     /locus_tag="PLB46_15395"
     CDS             complement(2215..>2439)
                     /gene="rplN"
                     /locus_tag="PLB46_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRF77963.1"
                     /translation="GGGIKKGTVSTAVVVRTHKEVRRKDGSYIRFDDNACVLLNASDE
                     PRGTRIFGPVARELRDKQFMKIVSLAPEVL"
BASE COUNT          605 a          510 c          418 g          906 t
ORIGIN      
        1 attacttttt cggataacat agctaatgct tttatttacc tgctgtttta ccagctttac
       61 gacgcaatac ttcatcagaa tatttaatac cttttccttt gtatggttca ggcttacgga
      121 acgatctgat tttcgcagca attgcaccta ataattgttt atcgttgctt tcgagaatga
      181 tagttgggtt tttacccttt tcactcacgg ttgacaattt cacctctttt ggtatcatca
      241 tcatgatagg gtgagagtaa ccaacagcga tttctaaaat ctgacctgtg tttgctgctc
      301 tgtaacctac ccctaccagt tcgagttctt ttttgaaccc ttctgataca ccttttacca
      361 tgttggcaat taatgcgcgg tataaaccat gcatagcttt atggcgtttt tgttcggttg
      421 gacgttccag tttaacaact gaaccttcag gtttaatagc gatgtcgcta tctaccttaa
      481 gtgtcaactc tccgcgttta ccttttacgg taacaacatt gttgtcagca accgaaactg
      541 taactcctgc cggtaattga atgggtaatt ttcctattcg tgacattgta aaaatctttc
      601 tgttttaatt aataaacgta acataatact tcaccaccta cattctggcg tttagcttct
      661 ttttcggtca ttacaccttt tgaagttgac acaatggcca aacctaaacc gctcagtact
      721 cgtggcattt caccaacacc tgcgtatttt cgcaatcctg gtttacttac tctcgtaaga
      781 cctttgatgg ctggggattt tgtaacagga tcgtatttta acgcgatctt gatagtaccc
      841 tgtggacctt cttcctcaaa tttatatctg aagatgaaac catgctcata caaaatttca
      901 gtaaggcgtt tcttcatatt tgaagcaggc acctcaacaa ttctgtgatt tgctttttga
      961 gcatttctga ttctggtcag aaaatcagct attggatctg taactttcat ttgccgtttt
     1021 tactatttta tttattacca acttgcttta gtcattcccg gaattttacc atataaggct
     1081 aaatcgcgga gtttgattct tgaaatacca aggtcactaa tgtatcctct tggacgaccg
     1141 gttaatttac aacggttgcg gtatcttacc tgtgaagaat tacgtggaag tttatctaat
     1201 tcttcccatt ttccttctgc cttcagttgc ttacgtaagt cagcataatg cgtaatcatg
     1261 cgctgacgtt tagtatttct tgcaattatt gatttttttg acattgtatt tttttatggt
     1321 ttgcggaatg gcattcctaa tccttttaat aattcgtatg cttctgcatc ggttttagct
     1381 gaggtaacaa aagtgatatc cataccactg attcttgtta ctttgtcgat atcaatttcc
     1441 gggaaaatga tatgttcagt tatacctaat gtatagtttc ctcttccgtc gaagctttta
     1501 tcgtttacac ctttaaagtc acgaacacgt ggtaatgcca ctgcaaacaa acgatccagg
     1561 aattcataca tgttttctcc tctcaaggtt actttcacac caattggttg tgcagaacgc
     1621 aatttgaaat tcgaaatatc ttttttcgat acggtagcaa cacatttctg accagttata
     1681 cgtgtcattt catcgattgc tacatcgatc atttttttgt cctgagtagc ttttccaacc
     1741 ccttgattta ttgaaatctt aaggagttta ggcagctgca tagagctttt atagttaaac
     1801 ttctcttgaa gagaaggaac tacttcttcg ctgtattttt tcttcagacg tggagttcta
     1861 gtttccatta tttaattatt tcacctgatt tattagttct tactattttg ccttccactt
     1921 ctttgcggtt cactctgatt ccttttcctg ttttaggatc aaccagctga acattgctga
     1981 tgtgaattgg cgcttctttt ttaatgattc cgccgttagg gtattgttga gttgggcggg
     2041 tatgttttga aatgatgtta acaccttcaa ccataacacg accggtagta taatttacct
     2101 caactacacg tccctttttg cctttgtcgt cgccggtaat aaccaatacc gtatcgtttt
     2161 tcttaacgtg gtattttggt ttgaatcggc tgtttgtatt ttttgttgcc ataattataa
     2221 cacctccggt gctaatgaaa cgattttcat aaattgttta tctctaagtt cacgagctac
     2281 aggtccgaaa atacgtgttc ctcttggctc gtcagatgcg ttcagtaaca cacatgcatt
     2341 gtcatcgaaa cggatgtatg aaccatcttt acgacgaact tctttatgtg ttcttacaac
     2401 aactgctgta gaaacagtac cttttttgat accacctcc
//
LOCUS       DAORKW010000552         2437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2589_length_2437_cov_3.972712, whole genome shotgun sequence.
ACCESSION   DAORKW010000552 DAORKW010000000
VERSION     DAORKW010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2589_length_2437_cov_3.972712"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..>2437
                     /gene="smc"
                     /locus_tag="PLB46_15400"
     CDS             86..>2437
                     /gene="smc"
                     /locus_tag="PLB46_15400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRF77964.1"
                     /translation="MKLSTLEIKGFKSFADKTVLHFNENITGVVGPNGCGKSNVVDAI
                     RWVLGEQKPTALRTEKMENLIFNGTKARKSSGMAEVSLTFENTRNLLATEFSTVTVSR
                     HYFKTGESEYRINGVTCRLKDIHNLFMDTGISTDSYSIIELGMLDDILHDKDNSRRKL
                     FEQAAGISKYKTRKKETLNKLSATQVDLDRVEDLLFEIENNLKELEKQAKRAQRYQKL
                     KGDYKDNSIDLAVLTLQSQKDVYKQLNEQQTAESDTKLQIEAEIQALEASIADKKTAL
                     IDKEQSLSKLQKDLNAVTNDIHTKENDSNLSKERIKFLEEKQVSLTKNISEAEAYIAS
                     TEGDIAGLEQRVAEEIERLGGIKQSLQKFLEELEKAKNDHDVFREKLETTQNIFKSAE
                     RELYDVDKKIAINKVRLENYHRELGNIESDKGSHGDKLDPLRTELVGYDAANVEAQTK
                     VDDLVKSENELKNTISALQKDIEKTRTELGNARRSLDAKKNEFNLTKNMFESFEGFPE
                     SIKYLKKNVPSMKESPLLSDVVSCKDDYKTAIESFLDPWLNYYIVQNMAHAIEAVNEL
                     ANKEKGRANFFLLSEFKNGNAAKPIDGVVAALDVISVNDKYSALVNHLLGNVYIVAGD
                     EDFAVVSAKISAAYPDVHLLHKSGKIAKSGKTITGGSVGLFKGKRIGREKTLETLEAA
                     IKQFQEEESVINNALKEKQTQLQELQQSSHDKAIQIAREELNKVVQQSIATKARIESL
                     EQLLQQMEDKKAAFTRQIQETDEESIKLNESHEKQMALTEERQR"
BASE COUNT          895 a          429 c          509 g          604 t
ORIGIN      
        1 atccacgatt gttgatttta atttcagtgc ttttttgatg atggcttggc agtacattta
       61 ctttgtagcc tgaggaaaag agactatgaa actaagtacg ttagaaatca agggatttaa
      121 aagttttgca gacaaaacgg tattgcattt taatgaaaac attaccggtg tggttgggcc
      181 taatggttgt ggcaaatcga atgtggttga tgctattcgc tgggtattgg gcgaacagaa
      241 gccaactgca ttaaggacag agaagatgga aaacctgatt ttcaacggga ctaaggctcg
      301 taaatcatca ggaatggcag aagttagcct taccttcgaa aacacccgaa acctcttagc
      361 aaccgagttt tctaccgtta ccgtttctcg tcactacttt aaaacaggtg aaagtgaata
      421 ccgcatcaac ggagtaacct gccgcttaaa agatattcat aacctgttca tggatacagg
      481 tatcagcacc gactcctact ctattattga gttgggtatg ctggatgata ttttgcacga
      541 caaagacaat tcgcgccgta aattatttga acaggctgcc ggtatttcta aatacaaaac
      601 acgcaaaaag gaaacactca ataaattgag tgcaactcag gttgaccttg atcgtgtaga
      661 ggatttgttg tttgaaattg aaaacaacct taaagaactc gaaaaacaag ccaaacgcgc
      721 gcaacgttac cagaaattga agggtgatta taaggataac agtatcgact tagctgtact
      781 caccctgcaa tcgcaaaaag acgtttacaa acaactcaac gagcaacaaa cggctgaaag
      841 cgatacaaaa ctgcagattg aagccgaaat tcaggcattg gaagctagta tagccgataa
      901 aaaaacagct ttaattgata aagagcaatc actcagcaaa cttcaaaagg acctcaatgc
      961 tgtcaccaac gatatacata ccaaagaaaa tgacagtaac ctcagcaaag aacgtatcaa
     1021 gtttcttgaa gaaaaacagg ttagcttaac taaaaatatc agtgaagctg aagcttacat
     1081 cgcatccaca gaaggtgaca ttgcaggctt agaacaacgt gtagctgaag aaattgaacg
     1141 tttaggtggt attaaacagt cgctccagaa atttttggaa gaactggaaa aagccaaaaa
     1201 cgatcatgat gttttccgtg aaaaactgga aaccacacaa aacatcttta aatcagctga
     1261 gcgcgaatta tacgatgtag ataaaaagat agccatcaac aaggtgcgtt tggaaaatta
     1321 ccaccgcgag ttaggcaata tcgaatcgga taaaggaagc cacggcgata aactagaccc
     1381 tttacgtaca gagttggttg gatatgatgc ggcaaacgtt gaagcacaaa caaaagttga
     1441 tgatttggtg aaatcggaaa atgaattaaa aaatacgatt tcagcacttc aaaaagacat
     1501 cgaaaaaacg cgcaccgaat taggtaatgc tcgcagaagt ctcgatgcga agaaaaatga
     1561 atttaatctt accaaaaaca tgtttgaaag ttttgaagga ttccctgaat ctatcaaata
     1621 cttgaaaaag aatgtgccaa gtatgaaaga atcaccgctg ctttcagatg tggtaagttg
     1681 taaagatgat tataaaacag ccatcgaaag tttcctcgac ccttggttaa attattatat
     1741 cgttcaaaat atggctcatg ccattgaagc ggtaaatgaa ttggccaata aagaaaaagg
     1801 tcgcgctaac ttctttttac tttcggaatt taaaaatggc aacgctgcaa aaccaattga
     1861 tggtgtagta gctgcattag atgtaattag tgtaaacgat aaatatagtg cactggtaaa
     1921 tcacttattg ggcaatgtat atattgttgc cggagatgaa gattttgccg ttgtaagtgc
     1981 aaaaataagt gctgcttatc ctgatgtgca tttattacat aaatcaggaa aaattgcaaa
     2041 gtccggaaaa acaattacag gtggttcggt aggtttattt aagggcaaac gaattggccg
     2101 tgaaaaaaca ttggagacac ttgaagctgc tattaaacag tttcaggagg aagaaagcgt
     2161 aattaataat gcccttaaag aaaaacaaac tcaattacag gaattacaac aatccagtca
     2221 cgataaggct attcaaattg cccgtgaaga attaaataaa gtagtgcaac aatcaattgc
     2281 aaccaaagcg agaatagagt cgctggagca gttgttgcaa caaatggaag ataaaaaagc
     2341 agctttcacc cgtcaaattc aagaaactga tgaagagagt atcaagctga atgagtcgca
     2401 cgaaaaacaa atggcgttaa cagaagaacg tcagcgc
//
LOCUS       DAORKW010000553         2434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2593_length_2434_cov_3.290458, whole genome shotgun sequence.
ACCESSION   DAORKW010000553 DAORKW010000000
VERSION     DAORKW010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2593_length_2434_cov_3.290458"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..>2434
                     /locus_tag="PLB46_15405"
     CDS             200..>2434
                     /locus_tag="PLB46_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRF77965.1"
                     /translation="MDYSIDLIQNIERKWNQKWKDSNLYKVTEDPKLPKYYVLDMFPY
                     PSGSGLHVGHPLGYVASDIISRFKRLQGFNVLHPMGFDAFGLPAEQYAIETGQHPAIT
                     TEKNINYFREQLSRMGFCYDWSREVKTCDPNYYKWTQWIFTQLFKSWYNPFTNKSEKI
                     DSLIAILNEEGTTRLFALDKCQVLIDAATWKNYDVAAREKLLMQFRLAYLDYADIWWC
                     EMLGTVLANDEVKDGVSERGGHPVEKIKMRQWFLRITEFADRLLEGLNRVDFSESMKE
                     MQRNWIGKSEGALVKFNVAGANEVIEIFTTRPDTIFGATFMVLAPEHELVEKITTAEN
                     RIAIEEYIKYVKSRSERDRMSEVKKVTGEFTGAYALNPFNGKSIPIYIAEYVLAGYGT
                     GAIMAVPSNDDRDFAFAQKFDLPVIPVVDQSAYPQVSREDKIGVMINSDMLTGLEVKD
                     AIQKMLSAIEEKGIGKRRVNYRLRDAGFSRQRYWGEPFPVYYENDVPHLLEDNELPLL
                     LPQVESYKPAGAATSPLSGITEWVNFAPGKMRETDTMPGYAGSSWYFYRYMDVHNDQF
                     FASREKIDYWQDVDLYIGGTEHAVGHLLYSRMWNKFLFDIGLVAKDEPFKRLVNQGMI
                     QGVSKFIYRLLLTISAGEKHKHYYDSDLSLMPSIFVSKNLGIDILNQNEDSEIYKSIL
                     IMANQILTITYMHTDAVITQLRVTEVRVASNCFVGENLNIKNLIYFKSSDEKVPFTEF
                     YNAHY"
BASE COUNT          769 a          390 c          520 g          755 t
ORIGIN      
        1 agaaccgcgg gtttgcgcct ctacggatgc ttcgcatcat taatattaga atcttcgaat
       61 gtcattccac ctcataaccc ggctgtttca ttggtacata ggtatccgcc tcggcggaca
      121 cattggtaca tcaacggctg tttccatcag cacatcacca cattatcaca tcagcacatt
      181 aattcagtac ctttgcccca tggattattc aattgacctt attcaaaaca ttgagcgcaa
      241 gtggaaccaa aaatggaaag attccaattt gtataaagtt accgaggacc ctaagttacc
      301 taagtattac gtacttgata tgttccctta tccaagtgga agtggattac acgttggtca
      361 cccgttaggt tatgtggcca gtgatattat ttctcgtttt aaacgtttac agggatttaa
      421 tgtattgcac ccaatgggat ttgatgcatt tggattacct gcagaacaat atgctattga
      481 aacaggtcag catccggcga ttacaaccga aaaaaatatt aattatttcc gtgagcaatt
      541 atcgcgcatg ggtttttgtt acgattggtc ccgcgaagta aaaacctgtg acccgaatta
      601 ttataaatgg acgcaatgga tttttacaca attatttaaa tcatggtata atccttttac
      661 caataaatca gaaaaaatag attcgttaat tgctatttta aatgaagaag gaactacccg
      721 tttatttgct ttagataaat gtcaggtgtt aattgatgca gcaacatgga aaaattatga
      781 tgttgcggct agggaaaaat tattgatgca atttcgattg gcttatttgg actatgcaga
      841 tatttggtgg tgtgagatgt tgggaacagt tttagcaaac gatgaagtga aagatggtgt
      901 tagtgaacgc ggaggacatc ctgtcgaaaa aataaaaatg cgtcaatggt ttttgcgtat
      961 taccgaattt gcggaccgct tgctggaagg cttgaaccgc gtagatttca gcgagagcat
     1021 gaaagaaatg caacgcaact ggattggcaa aagtgaagga gctttagtta aatttaatgt
     1081 tgcaggcgct aatgaggtta ttgaaatatt taccacaaga ccagatacta ttttcggagc
     1141 gacatttatg gtgttggcac cggaacatga attagtggaa aaaattacta ctgccgaaaa
     1201 cagaattgca attgaagaat atattaaata cgtaaaaagc agaagtgaac gcgatagaat
     1261 gagtgaagta aaaaaagtga ccggcgaatt taccggtgct tatgcactca atccttttaa
     1321 tggaaaatct attccgattt atattgctga atatgtgttg gccggttacg gaactggagc
     1381 tataatggct gtgccaagta atgatgaccg tgattttgcg tttgcacaaa aatttgattt
     1441 gccggtaatt cctgttgttg atcaaagcgc atatccgcaa gtttcacgcg aagataaaat
     1501 tggtgtgatg attaattccg atatgttaac aggtttggaa gtaaaagatg ccatccaaaa
     1561 aatgttatcg gctattgaag aaaaaggtat tggtaaacga cgtgtaaatt atcgtttgcg
     1621 tgatgcaggg ttttcgcgtc aacgttattg gggtgaaccg tttcctgttt attatgaaaa
     1681 tgatgttccg catttattag aggataatga attaccattg ttattaccgc aagtagaaag
     1741 ttataaacct gccggtgcag caacatcgcc gctttcaggc attaccgaat gggtgaattt
     1801 tgcaccggga aaaatgcgtg aaacagatac aatgcctggt tatgccggtt cgagctggta
     1861 tttttatcgt tatatggatg tacataatga tcaatttttc gcatcgcgtg aaaaaattga
     1921 ttattggcaa gatgttgatt tatacatagg tggtaccgaa cacgcagttg ggcatttatt
     1981 gtattcgaga atgtggaata aatttttgtt cgatatcgga ctcgttgcta aagatgaacc
     2041 atttaaacgt ttggtaaatc agggaatgat tcagggggtt tcgaaattta tatatagatt
     2101 attacttaca atttctgcag gagaaaagca taagcactat tatgattcag atcttagctt
     2161 aatgccttcg atttttgttt ctaaaaattt ggggattgac atattaaatc aaaatgagga
     2221 ttcggagatt tataaatcaa ttttaataat ggcaaatcaa atcctcacta tcacttacat
     2281 gcacactgat gccgttatta ctcaattaag agttacagaa gtaagggttg catctaactg
     2341 ctttgttggg gaaaatttga acattaaaaa tttaatttat tttaagtcaa gtgacgaaaa
     2401 ggtaccgttc acggaatttt ataatgcaca ttat
//
LOCUS       DAORKW010000554         2427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2604_length_2427_cov_4.732715, whole genome shotgun sequence.
ACCESSION   DAORKW010000554 DAORKW010000000
VERSION     DAORKW010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2604_length_2427_cov_4.732715"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PLB46_15410"
     CDS             complement(<1..465)
                     /locus_tag="PLB46_15410"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRF77966.1"
                     /translation="MRKLTIILYLLVFSNQTLLQAQKTAIQGTVMATDSVTPLQGVDI
                     FLEQSNMFTTSNSNGVFTLVGVADGNYNLICSAVGYSTQRITVQVQNGLSSILNIYLK
                     EQINQLNEVVVMSGGDRSDVILCGSVQYISPKEIEAFRYSDVNRVLRLAPGTN"
     gene            881..1969
                     /locus_tag="PLB46_15415"
     CDS             881..1969
                     /locus_tag="PLB46_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77967.1"
                     /translation="MINGAWNNPAVWSTDGITPCACTPGYILSNTNIVINHTSTLSTD
                     LNIRFDATVEISTLGIINGSTRDVVVENGGYIETFGPINVASIEIELGGDGIFHDVVT
                     CVDKFRVEGHAQIDTLVTVLDGDIEVKETGVLDILVPYIEVDVPNGEFKVDGILNMNN
                     TCLYILNGDFRNKITGTINGSQYIEVMNGEIRDEGVWSASVSWCASGLATTMPFAENC
                     GGCNLLLPIEITAFTATMQNQLIALSWEIPNKLPTLHFDIYRLVETNTTWEKIASVIN
                     SSLITDFYFYDAPEQSGYHYYQLGFFDESLAYHKSEIISIMNPVQNKIIYGYYTLSGN
                     KITMDYHQLPFGIFIESSNMGNTLVIIK"
     gene            complement(2034..>2427)
                     /locus_tag="PLB46_15420"
     CDS             complement(2034..>2427)
                     /locus_tag="PLB46_15420"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77968.1"
                     /translation="LMQINSCEIIFYVADQNRSRDFYAALLQQEPILDVPGMCEFQLM
                     PNLKLGLMPENGIAKILLPVTVHPNTGNGIPRNELYIIVEDVNTVFNNAIAKGAILVS
                     AIEKRDWGDTVGYIQDFDGHIIAIAAHA"
BASE COUNT          764 a          432 c          437 g          794 t
ORIGIN      
        1 attagttccg ggagccaaac gcaatactct gtttacgtca gaataacgaa atgcctcaat
       61 ttcttttgga gagatgtact gcaccgaacc acaaagaatt acatcactcc ggtcaccacc
      121 actcataaca acaacttcat tcagctgatt tatttgttct ttcaaataaa tattcagtat
      181 agagcttaaa ccgttttgca cctgtacagt aatgcgttga gtactgtagc cgactgctga
      241 acaaatcaag ttataattgc catctgcaac acctaccaag gtaaatacac cattactgtt
      301 tgaagtggtg aacatattgc tttgttccag aaaaatatct accccttgaa gtggtgtaac
      361 cgaatcggtg gccatcaccg tgccttgaat tgctgttttt tgagcctgta acagcgtttg
      421 gttgctaaat accagtaggt ataatataat agtcagtttg cgcattgcgg ccacaaagtt
      481 caatccctgg cagcgacagg gcaataccta tatgaaggta ttttagaatt tgtctaaata
      541 acgaattggc tacagtccag gggaaaccct atgaatgcta cccattttgc ctacatcaaa
      601 tagggcttcc aaataacaac aaagttggtt tattaagccc aggagcaatt ttttagttaa
      661 aattatttgt atatacatat aatttagtta tatttattac ttcttctcgc aggcacacat
      721 catcttctat tacaccgcaa gcaacaatta aatgatttga ttttcaaacc ataacggatt
      781 gcttatgtat atttttaaac taaaaacaaa gctggctttt cagtttcttt tattcttatt
      841 atgttactgt caggttaatg cacagataac ctatacaact ttgataaatg gtgcttggaa
      901 caaccctgcg gtttggtcaa cagatggtat tacgccatgt gcttgcacgc cgggatatat
      961 tttaagtaat acaaatattg taattaatca taccagtaca ttaagtaccg atttaaatat
     1021 tcgttttgat gcaactgttg aaatcagcac ccttggtatt ataaatggtt caacacgcga
     1081 tgtagtggtt gaaaacggag ggtatattga aacatttggc cctatcaatg tagcatctat
     1141 tgaaattgaa ttgggtggtg atggaatatt tcatgatgtt gttacttgtg tagataaatt
     1201 cagggttgaa ggtcatgcac aaattgatac attagttact gtgttggatg gagatattga
     1261 agtaaaggaa accggtgtat tggatatttt agtcccttat attgaagttg atgttccaaa
     1321 tggtgaattc aaggtagatg gaatactcaa catgaataat acatgtttat atattttgaa
     1381 tggtgacttt agaaataaaa ttaccggcac tattaacggc agtcaatata ttgaagtaat
     1441 gaacggagaa attcgcgatg aaggtgtttg gtcggcaagt gtttcctggt gcgcaagtgg
     1501 attggcgaca acaatgccat ttgcggaaaa ttgtggtgga tgtaacttat tgttacctat
     1561 tgaaattact gcctttacag caactatgca aaaccagcta attgctctat catgggaaat
     1621 tcccaataaa ttacctacac tccatttcga catttatcgg ttagttgaaa cgaatactac
     1681 atgggaaaaa attgcatctg ttattaattc ttcccttata acagattttt atttctatga
     1741 tgcacctgaa caatccggct accattatta tcagttaggt ttttttgatg aatctttagc
     1801 atatcacaaa agtgaaatca tcagtattat gaatcctgtt caaaacaaaa ttatatatgg
     1861 atattacacc ttgagtggga ataagataac tatggattac catcaattac cgtttggaat
     1921 ttttatcgaa tcttccaata tgggaaatac cttagtgatt attaaataac agacgaattc
     1981 aatcggttat gacatgcgct gtatcatatt agaacaaaat gtcttaaatg aatttatgcg
     2041 tgtgcggcaa tcgcaataat atgcccatca aaatcctgga tgtaacctac tgtatcgccc
     2101 caatctcttt tttcaattgc acttactaat attgcaccct tagctattgc attgttaaat
     2161 acggtattta catcttcaac aattatatac aattcattgc gtgggattcc gtttcctgtg
     2221 ttcgggtgta cggtaactgg caataatatt tttgcaatac cattttcagg cattaatcct
     2281 aatttcaagt ttggcattaa ttgaaattca cacatgcccg gcacatcaag tataggctcc
     2341 tgctgcaata atgctgcata aaaatcacga ctccgatttt ggtcggcaac gtaaaaaatt
     2401 atttcgcagg aattaatttg catgagt
//
LOCUS       DAORKW010000555         2427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2606_length_2427_cov_4.286256, whole genome shotgun sequence.
ACCESSION   DAORKW010000555 DAORKW010000000
VERSION     DAORKW010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2606_length_2427_cov_4.286256"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLB46_15425"
     CDS             complement(<1..501)
                     /locus_tag="PLB46_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77969.1"
                     /translation="MKNFIIQFFISLLLIPCSFSQAPAIEWQKTIGSSLSETLYSIEQ
                     TTDGGYILGGRSAFGISGDKTEEGYGGFDYWIIKLDLTGNIEWQNVIGGSADDVLITI
                     NQTSDGGYIAGGYSSSGISGVKTEINFGGPDFWIVKLDASGNIVWENTIGGNWYDYYT
                     CLDQTED"
     gene            complement(524..2038)
                     /locus_tag="PLB46_15430"
     CDS             complement(524..2038)
                     /locus_tag="PLB46_15430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77970.1"
                     /translation="MFDNLYSAHETADGGFILGGTSTSGISGDKTEALIGSIDMWIVK
                     LDASGTVIWNKTIGGTGAEYVYSVIPTADGGSIAAAISDSGVSGNKTEATNGGNDYWI
                     VKLDNSGNITWQNSIGGSGSDLLFVNAIDQTSEGGYAVAGYSQSLISGDKTEANNGSW
                     DYWILKLNNSGSIIWQSTIGGTSGDYVNAIRATADEGFIVGGYSYSNAGGDKEEDAYG
                     DADYWVMKLEGTGCYAFAETCNGIDDDCDAIVDEDVTETISIIATGPTTFCQGGNVVL
                     NATHSGTSLQWKKNGINISGATASTYFASTTGNYTCETTSICNTAISNSITVTVNKNP
                     KASITAGGPTSFCAGGSVTLTEMPVGGSTYQWYKGATPLAGATSNVYVATTAGNYKCR
                     VTKTATGCFKNSNAIAVSVPCREGELIEENNLFSIYPNPTKNSFTISAELNSNSNSNL
                     VIYNQLGQIIYSIKINNTDGIISELIQFENQPSGIYLVKIFNNNLQFEQKLIIQ"
     gene            complement(2201..>2427)
                     /locus_tag="PLB46_15435"
     CDS             complement(2201..>2427)
                     /locus_tag="PLB46_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77971.1"
                     /translation="WENTVGGGNFDRFYSVEETADGGFILGGQSLSGGGWGDKSESNK
                     GGYDYWIVKLNSDGIGRWDRGWGGAGKDQL"
BASE COUNT          715 a          561 c          340 g          811 t
ORIGIN      
        1 atcttcagtt tgatccaagc aagtatagta atcgtaccaa ttccctccga tcgtattttc
       61 ccaaacaata tttcccgatg catccaattt tacaatccaa aaatccggtc caccaaagtt
      121 gatttctgtt ttaacgccag atatccccga agaggaatag ccgcctgcta tataaccacc
      181 atcactcgtt tggttaattg tgattagcac gtcgtcagca cttcctccaa tcacattttg
      241 ccattcaata tttcctgtta gatctagttt aatgatccag taatcaaatc caccgtatcc
      301 ctcttcagtt ttatcaccgc taattccaaa tgcacttctg ccacccaaaa tatatccgcc
      361 atcagtagtt tgttcaatgc tgtataaagt ttcgcttaaa cttgaaccaa tggttttctg
      421 ccattcaatt gcaggagctt gggaaaagga gcaaggaatg aggagcaagg agataaaaaa
      481 ttgtataatg aaatttttca taagaaatat ttaaattatc tacctattgg ataattaatt
      541 tttgttcaaa ttgtaaattg ttgttgaata ttttcactaa ataaattcct gatggttgat
      601 tttcaaattg aattaattca cttataattc catcagtatt atttattttt attgaataaa
      661 taatttgacc tagttgatta tatattacaa gattagaatt tgaattgcta tttaattctg
      721 cagaaattgt aaaactattt tttgtcggat tcggataaat agaaaataaa ttattctctt
      781 ctattaattc cccttcccta caaggcacac ttaccgcaat cgcattagaa tttttaaaac
      841 aacctgttgc agttttggta acacgacatt tataattacc ggctgtagtt gctacatata
      901 cattagatgt tgcacctgct aatggtgttg cacctttata ccattgataa gtgctgccac
      961 ctacaggcat ttcggttaat gtaactgacc caccggcaca aaatgaagtt ggacctcctg
     1021 ctgttatcga agctttcgga tttttgttaa cagtaactgt aatactgtta gaaatagctg
     1081 tattacatat acttgtcgtt tcacatgtat aattacccgt tgtacttgca aaataagtag
     1141 atgccgttgc accggaaata tttataccgt ttttcttcca ttgtaaagat gttccagaat
     1201 gtgttgcatt taatacaaca tttcccccct ggcaaaatgt agtaggtcct gttgctatga
     1261 ttgaaattgt ttcagtaaca tcttcatcta caatagcatc acaatcgtca tcaattccat
     1321 tacaggtttc tgcaaatgca taacatccgg taccttctaa tttcataacc caataatctg
     1381 catcaccata cgcatcctct tccttatcgc ctcctgcatt actatatgaa taaccaccaa
     1441 caataaatcc ctcatcagca gttgctctga tagcatttac ataatcgccg cttgttccgc
     1501 caattgtaga ttgccaaata atacttccgg aattatttaa ttttaaaatc caataatccc
     1561 aagaaccatt atttgcttca gttttatcgc cactaattaa actttgagaa tatccggcta
     1621 cagcataacc accctcgctg gtttgatcga tggcatttac aaacaacaaa tcacttcctg
     1681 aaccacctat tgaattttgc caggtaatat ttccactgtt atctaattta actatccaat
     1741 aatcgtttcc gccattggta gcttcagttt tatttcctga aacaccacta tcggaaattg
     1801 ctgctgctat tgaaccacca tcagcagttg gaataacaga ataaacgtat tctgcacctg
     1861 ttccaccaat tgttttattc caaattacag ttcctgaagc gtctaatttt acaatccaca
     1921 tgtcgatact acctattaat gcttcggttt tatcgccact tattccactg gtagatgttc
     1981 cacctaaaat aaatccacca tcagctgttt catgagcaga ataaagattg tcgaacataa
     2041 ctccgccaat atcattttgc cattgaatat tgccattccc atctgtttta attacccaat
     2101 aatcggaatt accaactctg ttttcagttt tatcaccact tattccgctg tcggtggctc
     2161 cggcaagaat aaatccgccg tcggatgttg gttgcgcatt ctacaactgg tccttccctg
     2221 cgccgcccca cccccggtcc catcgcccaa taccgtctga atttaatttg acaatccaat
     2281 aatcatatcc ccctttatta ctttccgatt tatcgcccca gccaccgcca ctcaaagatt
     2341 gtccgcctaa aataaatcct ccgtctgctg tttcttcaac tgaataaaat ctgtcaaaat
     2401 ttccaccgcc tacggtattt tcccatt
//
LOCUS       DAORKW010000556         2427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2607_length_2427_cov_3.575042, whole genome shotgun sequence.
ACCESSION   DAORKW010000556 DAORKW010000000
VERSION     DAORKW010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2607_length_2427_cov_3.575042"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PLB46_15440"
     CDS             complement(<1..841)
                     /locus_tag="PLB46_15440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRF77972.1"
                     /translation="MYSNNLLTSGKTHYDPFGMLLEGRNWEGGSEYRYGFNGKESDKE
                     TYGDGNVYDYGFRIYNSRLGKFLSVDPLFKSYAELTPYQFASNRPIDGIDLDGLEYIT
                     YIISVENNTGVSKIESIDFRDAESFNYAKYSQSYGPEGRGVKYIYNYVDENGTPTGKT
                     KTRWAQEQGGWNSVGLSKYIYHGLFNGKGAISTVGPLFPIDHGTGTNTYRWDETPIDM
                     PDAIAREHDLAMDVEGYQPSDWKDPENIFADISIVKQAELYLKESSSPGYEDPFSATG
                     TVSD"
     gene            complement(884..1939)
                     /locus_tag="PLB46_15445"
     CDS             complement(884..1939)
                     /locus_tag="PLB46_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRF77973.1"
                     /translation="MIYNFETLFIRFIVSSITGRHKQLNGKRLRKSVITKYKDTLHSI
                     VLFESELNIDLHICDLKRAGKNLLKSEIKRWEKISAKYAKFLFKRGCYDNYVSSHFKI
                     IKVFLRYLTVEKLIDCTVIINCFKSVKEQIPIIVLEQKHIVFLTGNEKFAATLNARFI
                     ATKDIFVAGCFVGLRFGDLMNLRPNNIQCSSGGNYLRVTSGKTNTETVLKLPDYIITI
                     FNKYKKTGKLLPQISNSQLNKNIKLLCEAAGWTSVIGKSRNQEGIPHTILKNGKPYRF
                     CDLITTHTMRRTAITNLLLLGVPELMVRQISGHAPGSKAFYRYVSFAQQYLDSAIDLV
                     HEKMNALIDAEKTQSKL"
BASE COUNT          752 a          456 c          376 g          843 t
ORIGIN      
        1 cgtcactaac tgttcctgtt gcagaaaatg gatcttcgta gccaggagat gaagattcct
       61 ttaaataaag ctctgcctgt ttgacaatac ttatatcagc aaaaatgttt tcaggatcct
      121 tccaatcact aggttgataa ccttcaacat ccattgctaa atcatgttca cgggctattg
      181 catccggcat atcaattggt gtttcatccc atctatatgt gtttgttccc gttccatgat
      241 caattggaaa taacgggcca acggtggaaa ttgcaccttt gccattgaac aatccgtggt
      301 agatatattt gcttaatcct accgaattcc atccaccttg ttcttgagcc catctagttt
      361 tagttttacc cgtaggtgtt ccattttcat caacataatt atatatatat ttcacacctc
      421 ttccttcagg tccgtatgat tgagaatatt ttgcataatt aaaactctca gcatctctaa
      481 aatcaataga ttctatcttc gaaacgccag tgttattttc gacagaaatg atatatgtga
      541 tatactccaa tccgtctaaa tcaataccat caataggtcg attacttgca aactggtaag
      601 gggttaattc cgcatatgat ttaaacaatg gatcaacact caaaaactta cccaacctag
      661 aattataaat cctaaaccca taatcataaa catttccatc tccatatgtt tctttatcac
      721 tttctttccc gttaaacccg tacctatact cactcccccc ttcccaattt cgtccttcca
      781 acaacatccc gaacgggtcg taatgagttt tgccagaagt caaaaggtta ttggaataca
      841 tgggattaat tattattcaa atattatatt attttactaa taatcaaagt tttgactgtg
      901 ttttttccgc gtcaattagg gcattcattt tttcatgaac caaatcaata gccgagtcaa
      961 gatattgttg cgcgaatgaa acatacctgt aaaaggcctt gctgcctggt gcgtgaccgg
     1021 aaatttgacg aaccattaat tcaggaacac ctaacaatag taaattagta atggctgtgc
     1081 gacgcatggt atgtgtggtt atcaaatcgc aaaagcgata tggtttgccg ttttttaaaa
     1141 ttgtatgtgg aataccttcc tggtttcttg atttgccgat aacggaagtc cagcccgctg
     1201 cttcacataa caatttgata tttttgttta actgactatt tgaaatttgg ggtaaaagtt
     1261 tgcctgtttt tttatattta ttgaaaatgg ttattatata atcagggagt ttaagtacgg
     1321 tttctgtatt tgtttttcct gatgtaacac gcaaataatt tccacctgag gagcattgta
     1381 tgttattggg ccttaggttc attaggtctc caaacctcaa acctacaaaa cagcccgcaa
     1441 caaaaatgtc tttcgttgca ataaatctgg cattcaatgt ggcagcaaat ttttcattcc
     1501 ccgttagaaa aacaatatgc ttttgttcga ggacaattat cgggatctgc tcttttacgg
     1561 atttaaaaca gttaattata actgtgcagt cgataagctt ttcaactgtt aaatacctga
     1621 gaaacacctt aataatttta aaatgtgaac ttacataatt atcgtaacag ccccttttga
     1681 ataagaattt ggcgtatttc gcacttatct tttcccatcg ttttatctca gatttcaaaa
     1741 ggttttttcc ggctcttttg aggtcgcaaa tatgtaagtc gatattcaat tcggactcaa
     1801 atagaacaat tgaatgcagc gtatccttat atttggtaat tactgatttt cttaatcgct
     1861 tgccattcaa ttgtttatgt cggccagtga tgctgctcac tataaatctt ataaataatg
     1921 tttcgaagtt gtaaatcata ttagataata tttaatttta tttagaggta ggtttgagat
     1981 aaattccata aataattgca aacattatct aatgaatcag ttatttatac tcctataaaa
     2041 attagttttg tatcctaaaa tgaaccttac ttttaagatt tacctttaca tggggcattg
     2101 attaacaagc atttagttga atatgtgaaa aaatgacaaa aatattttca aaataatttg
     2161 tttttttaaa aattgattgt taaacttgct tagaaaatag ttgaaaccct tatacaataa
     2221 ccctattaaa caacaattaa aacggagaat tgagaaccct ccaaattgaa aacaatccaa
     2281 tttatttttg gctgacaatg cactttcatc atccccgttg gctaacctat tttaattact
     2341 gtcacgtttt gcgacaaacc tagtgttgct aaggctacaa aggatttgaa ctgatttgtt
     2401 tacaaatcgg acagctaaac tattatt
//
LOCUS       DAORKW010000557         2424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2610_length_2424_cov_4.618404, whole genome shotgun sequence.
ACCESSION   DAORKW010000557 DAORKW010000000
VERSION     DAORKW010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2610_length_2424_cov_4.618404"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLB46_15450"
     CDS             complement(<1..246)
                     /locus_tag="PLB46_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase"
                     /protein_id="HRF77974.1"
                     /translation="MENTKKDILWRLYLILAGMVIVGILIVVQIGNVQFVHGDEYRAM
                     ADSMRTKQQEIKPTRGSIYSEHHDVLATSFPYFNIYMD"
     gene            complement(233..601)
                     /locus_tag="PLB46_15455"
     CDS             complement(233..601)
                     /locus_tag="PLB46_15455"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HRF77975.1"
                     /translation="MSEKQNTGKGAENKRSLSSMLKSFDEVKFFQWENIVNNIPYFMF
                     IVIIGIFYIWNNHRGVAMDREIRTVNQELLEKQYYFNATRDSLTQHSRQSAVARRVDT
                     LNMQELSNPPYTIKPKSGKH"
     gene            complement(621..1541)
                     /gene="rsmH"
                     /locus_tag="PLB46_15460"
     CDS             complement(621..1541)
                     /gene="rsmH"
                     /locus_tag="PLB46_15460"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRF77976.1"
                     /translation="MSTYHTPVLLHTSIQALITDPDGIYVDVTFGGGGHSKEILRYLS
                     PKGKLYCFDQDADAQNNFINEFDATNATFIPQNFRFISKFLRVQGVTGVNGILADLGV
                     SSHQFDEGNRGFSIRFDGPLDMRMNTSQPVSAFDIINRYTEEQLQNIFSLYGEIRNAK
                     QLANAICAARLQLRSEGGIKTTAQLREIALTVVKGEKNPYLAQLFQSVRIEVNEEMLA
                     LKEMLINAAQLIIPGGRLVVISYHSLEDRMVKNMMKTGDVYGNPEEDLMGRKNIPFKV
                     VTKKPVTPEQDELQKNPRSRSAKLRVAEKI"
     gene            complement(1538..1993)
                     /gene="mraZ"
                     /locus_tag="PLB46_15465"
     CDS             complement(1538..1993)
                     /gene="mraZ"
                     /locus_tag="PLB46_15465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRF77977.1"
                     /translation="MAFLIGEYECTLDVKQRIRVPAGLVSQLSGDDVGKFVITRGVDP
                     CLYLYPMSEFYTEMDKINQIPENSEEDRDFKNMFYSGTSILTIDSAERILIPRLHVQH
                     AGITKDMIMVCQKNKVVVWAMEKFREKYLNRNPADYQQLSEKVRGKYGI"
     gene            complement(2219..>2424)
                     /locus_tag="PLB46_15470"
     CDS             complement(2219..>2424)
                     /locus_tag="PLB46_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77978.1"
                     /translation="GRRVAGVWVHHKPVVKVEKRLESFPNAVFVADNTNSWKSNSYWK
                     LHFEGSGVHFHSLRDQGALEVGM"
BASE COUNT          671 a          514 c          477 g          762 t
ORIGIN      
        1 atccatataa atattaaaat agggaaagga agttgccaaa acatcgtggt gttcactata
       61 aatagagccg cgtgttggtt taatttcctg ttgtttggtg cgcatgctat cggccattgc
      121 acgatattca tcaccatgca caaattgcac attaccaatc tgtacaacaa tcagaatacc
      181 cactatcacc atccctgcaa ggatgagata caatctccat aatatgtcct ttttagtgtt
      241 ttccactttt tggttttatg gtataaggtg ggttagataa ttcctgcata tttaaagtat
      301 ccaccctgcg cgcaacagca ctttgtctgc tgtgttgcgt aagcgaatcg cgtgtggcat
      361 tgaaataata ttgtttctcg agtaattcct gatttaccgt tcttatttct ctgtccatgg
      421 caacaccacg gtgattattc cagatataaa aaataccaat gatgacaata aacatgaaat
      481 agggaatatt attgacaata ttttcccact gaaaaaactt cacttcatca aatgatttta
      541 gcattgatga caggctacgt ttattttcag cacctttgcc ggtgttttgt ttttctgaca
      601 ttgttggtta ataaaagcga ttatattttt tcagcgacgc gcaatttagc actgcgtgaa
      661 cgcggatttt tttgtaattc atcctgttca ggagttaccg gttttttggt aactacttta
      721 aaaggaatat tttttctgcc cattaaatct tcttccggat taccatacac atcacctgtt
      781 ttcatcatat ttttcaccat acgatcttcc agtgagtggt aactgataac aaccaatctt
      841 ccaccgggaa taattaattg agcagcatta atgagcattt cttttaaggc aagcatttct
      901 tcgttcactt caattcgcac cgattgaaat aattgagcga ggtagggatt tttttctcct
      961 tttacaacgg tgagtgcaat ttcacgtaat tgagcagtgg ttttaattcc accttcactt
     1021 ctcagttgta atcttgctgc gcagatggca ttagcgagtt gttttgcatt acgtatttca
     1081 ccatataagc tgaaaatatt ttgcaattgc tcttctgtgt aacgattaat tatatcgaat
     1141 gcagaaaccg gttgcgacgt gttcatgcgc atgtcgagcg gaccatcaaa acgaatggaa
     1201 aaaccgcggt taccttcatc aaattgatgt gagctaacac cgaggtcggc taaaatgcca
     1261 tttactccgg taacaccctg cacccgcaaa aatttagata tgaatctaaa attctgcggg
     1321 ataaaggtgg cgtttgtagc gtcaaactca ttgatgaaat tattttgtgc atcagcatcc
     1381 tgatcgaaac agtagagttt tccttttgga gaaaggtagc gcaaaatttc tttgctgtgt
     1441 ccaccgccac cgaaagtaac atccacataa attccgtcag gatctgtaat gagtgcctga
     1501 atactcgtat gtaaaagaac gggtgtgtga taagtgctca tataccgtac ttgccacgca
     1561 ctttttcaga taattgttga tagtccgcag gatttctgtt gaggtatttt tcacggaatt
     1621 tctccattgc ccataccacc actttgtttt tctgacaaac cattatcata tcttttgtaa
     1681 ttccggcatg ttgaacatgt aatcgcggaa tcagaatgcg ttcggctgag tcgatcgtta
     1741 aaattgaagt cccactgtaa aacatgtttt taaaatcgcg gtcctcctca gagttctcag
     1801 ggatctgatt gattttgtcc atttctgtgt agaattcact catggggtat agataaaggc
     1861 atgggtcaac tccacgagtt attacgaact ttccgacatc atcaccggag agttgtgaca
     1921 ccagaccggc aggaacgcgg atgcgctgct ttacgtctaa ggtgcactca tattctccta
     1981 ttaaaaatgc catagttggt cgttatgaac taattcgaac gtaaacttag aagttttatt
     2041 acactttcgt acacttttgt acactttcga ggttgaaaaa tttttgcaca aataatgcac
     2101 atcggttatg cacatttgtt agcattggcg cggagtgcaa gctgggcggg cattgagacg
     2161 catcaaaaaa tggcttggac ggaaaaatag gagaaattga cggcgttttg tgacagtttc
     2221 acattcccac ttccagtgca ccttggtccc tcaaggagtg aaaatgtaca cctgagccct
     2281 caaaatggag cttccagtag ctattactct tccaactgtt ggtgttatcg gccacaaaaa
     2341 ccgcattggg aaaggattcc agccgtttct ccaccttgac cacaggcttg tgatgcaccc
     2401 acacacccgc cacccggcgg cccc
//
LOCUS       DAORKW010000558         2414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2633_length_2414_cov_5.183128, whole genome shotgun sequence.
ACCESSION   DAORKW010000558 DAORKW010000000
VERSION     DAORKW010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2633_length_2414_cov_5.183128"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLB46_15475"
     CDS             complement(<1..324)
                     /locus_tag="PLB46_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77979.1"
                     /translation="MKYLLFTIGLLLFISNLYTQSYNIASYNGLTVNTCSGNFYDSGG
                     SGGNYGNNENYVVTFCSSSGSPLYFDFGASSTSLNLDAGTGDTLFFYDGLTTSDPLIA
                     TLTKSD"
     gene            421..1923
                     /locus_tag="PLB46_15480"
     CDS             421..1923
                     /locus_tag="PLB46_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77980.1"
                     /translation="MKPSDAFELIQSLSMSEKRQFVVYANRHVIGDINKYLQVFHLLQ
                     EMTVYDEQLLMTLVKQQGLATKYLKADQHYLYQLLLESLVMQHNNKSANLLVKSALLK
                     IEILFDKGLYRQCKKELFKAITVAIKYELFEYHINLLFWERKTQVMSPDKLYDFRALH
                     EKTAQAITQLQQFTTYTMLYDKAVELRQSVIKTRTPSAIQKFKKLLHHDLLLSPAGKS
                     VQMQIRYHQIYAMWYYIHKDKTNELLHNKQIIQLLDKHAHFKEEYPLDFIAIYSRILS
                     ITKSGPAPMFEKELLAFRAFKPLSQSITYDNIVAQIFTQSYMIQVSQLLSNKSFYTAG
                     RILPELQTGIQYHKRFINPVALLSLYFITAYTAFANGNLALARKYVNYIINEFDDSLR
                     PDIYNFSRLLNLMLHLELRNYSNIKSEYTSVSYYFNKNKKLFKTENLVLKYFSNPKNY
                     MYNSNGALLVLQDNLEKIKEIKIEQFALNYIDFFTWIKARLSRQPMAEVK"
     gene            complement(1938..>2414)
                     /locus_tag="PLB46_15485"
     CDS             complement(1938..>2414)
                     /locus_tag="PLB46_15485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77981.1"
                     /translation="SHNSFTSVSQMVFVDKLHGFLVQGNWKDWIYSTDDGGVSWSISY
                     TAPESINKLKVTPNYIYAVGNSGMLLRYQITTTDIESPVTNTFNAYPNPTSQMIYLDA
                     PASGLNYSITNVLGEVVLTGKTTSVLQQIDISQFATGMYVVINNDNIRDNRIFIVE"
BASE COUNT          838 a          441 c          383 g          752 t
ORIGIN      
        1 atcgctttta gttaaagtag ctattagcgg atctgaagta gtgagaccat cataaaaaaa
       61 cagggtatct cctgttcccg catccaaatt taaagatgtg gaacttgcac caaaatcgaa
      121 ataaagcggc gaccctgatg atgaacaaaa cgtaactaca taattttcat tgttaccata
      181 atttccacca cttccaccgg agtcgtaaaa attgcctgag caggtgttta ccgttaggcc
      241 attatacgac gcgatattat acgattgcgt gtataaatta ctgatgaata acaatagccc
      301 tattgtaaat aacaagtatt tcataattgc cctgttctga tgtttacaaa aatatagtgc
      361 ccgggatttg gtttttacaa aaaatatgcg ttttttagta agtctgattt tgcctttatt
      421 atgaaacctt ccgatgcttt tgaactcatc caatcactca gcatgtctga aaaacggcag
      481 tttgtagtat acgcaaaccg tcacgtaatt ggtgacatta ataaatacct ccaagtattt
      541 catctgctgc aggaaatgac tgtttatgat gaacagctac taatgacatt agtaaaacag
      601 caaggattgg caactaaata cctaaaagct gaccaacatt atttatacca gctattattg
      661 gaatctctgg ttatgcagca taacaacaag tctgcgaatt tattggtaaa atcagcgttg
      721 ctaaaaattg aaatattatt tgacaaagga ttataccggc agtgtaaaaa agaattgttt
      781 aaagcaatta ctgttgcaat aaaatatgaa ttgtttgaat accatatcaa cttattattt
      841 tgggaacgca aaacacaagt aatgagcccg gataaactgt atgactttcg cgctttacat
      901 gaaaaaaccg cccaggcaat aactcaattg caacaattta caacttatac catgctttat
      961 gataaagctg tagaactgcg acaaagcgta ataaaaacca gaacaccatc agctatacaa
     1021 aaattcaaaa aattactaca ccatgattta ttgttgtcac ctgcaggtaa atctgtccaa
     1081 atgcaaatac gttaccacca gatttacgca atgtggtatt atattcataa ggataaaaca
     1141 aatgaattat tgcacaataa acaaatcatt caattattag acaaacatgc acatttcaaa
     1201 gaagaatacc cactcgattt tattgcgatt tattcaagaa ttttatctat caccaaatcc
     1261 ggaccggcgc caatgtttga aaaagaattg ttggccttca gagcttttaa accactaagt
     1321 caaagtatta cttacgacaa tattgttgca caaattttta cgcaatcgta tatgatacag
     1381 gtgtctcagt tgcttagcaa caaatcgttt tatactgccg gacgcatatt gcccgaatta
     1441 cagacaggta ttcaatatca caaacgattt attaatccgg tagcattatt atcactctac
     1501 tttattaccg cttataccgc ttttgccaat ggcaatttag cacttgcaag gaaatatgtg
     1561 aactacatca tcaatgaatt tgacgatagt ttaagaccgg atatttataa cttttcaagg
     1621 ctcttgaatt tgatgttgca cttagagctc aggaactatt ccaacattaa atcggagtat
     1681 acttcggtga gttattattt taataaaaac aaaaaactat ttaaaacaga aaatctggtg
     1741 ttaaaatatt ttagcaatcc caaaaactac atgtacaatt ctaacggagc cttattagtg
     1801 ttacaagata atttagaaaa aattaaggaa ataaaaatcg aacagtttgc tttgaactat
     1861 attgatttct ttacatggat aaaagcccga ttatcacgac aaccaatggc cgaggtaaaa
     1921 taattattga cccttgttta ttccacaata aatatacggt tatcccgaat attatcatta
     1981 ttgataacga catacatacc tgttgcaaat tggctgatat ctatttgctg aagaacggat
     2041 gttgttttac ctgtcaatac aacctcacct aaaacattgg ttattgaata attaagcccc
     2101 gatgcaggtg catccaaata tatcatttgt gatgtgggat tgggatacgc attaaaggta
     2161 ttcgttacag gtgattcgat atcagttgtg gtaatttgat atcttaataa cataccacta
     2221 tttcctactg catatatata attgggggta acttttaatt tattaatgct ttctggtgca
     2281 gtgtaactaa ttgaccaact tacacctccg tcatcggtgc tataaatcca gtccttccaa
     2341 tttccctgca ctaaaaaacc atgtagttta tctacaaaca ccatttggct tactgatgtg
     2401 aatgaattat ggct
//
LOCUS       DAORKW010000559         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2649_length_2399_cov_4.080205, whole genome shotgun sequence.
ACCESSION   DAORKW010000559 DAORKW010000000
VERSION     DAORKW010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2649_length_2399_cov_4.080205"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(350..1732)
                     /locus_tag="PLB46_15490"
     CDS             complement(350..1732)
                     /locus_tag="PLB46_15490"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77982.1"
                     /translation="MKYYYAFFLLLFTIKAEAQFTKLFDFNDSFGINPSSSLIYDGVY
                     LYGMTTNGGTGTNCLTGDCGVIFKIKPDGSGYEKILDFDEYNGAFPHGSVILVGEFLY
                     GMTYKGGEHGSGIVFRIKTDGSNYEKILDFDGDNGSAPKGELYYDGNYLFGMTLGGGA
                     TDHGTIFRINPDGTDYTRLHDFTGSVTGNGPYGTLISDSVFLYGTTYGGGIFDKGTIF
                     KVKPDGTEFTSLYSFDGLTGRNPWGNLLSDGNFMYGMTYNGGNYGFGAIFKIKSDGSE
                     FTIIHDFDDNHGSHPEGNLIFYRGKLYGLTHWGGTSNNCSFECGVLFKLNTDGTGYSV
                     LVNFNETTGSDPKGELITINSTFYGMTYQGGEYRRGTIFKYEIAKEIDLNDNDLIFPN
                     PFTNSLNLSGTTADGSIIIYDITGRAIIRQQSYESTTMINANNIASGLYLLQYIDQEL
                     NIKTYKLVKN"
BASE COUNT          736 a          494 c          397 g          772 t
ORIGIN      
        1 catccgaata gccagacatt accatgtatc caccgagcac cgcttgaaaa tcctggggag
       61 tataggttgt tgttaatttg ttgaatttct tattctcata gtctctaacc ccattgtttg
      121 gtcaggggac acaaccaatg ggtgagcttc gaatgatagg tggtgctatg gcaaatactt
      181 ttttccttcg atagcaatac cttcgaagca accctgtaaa tcacttcgaa tcatcccaat
      241 aattgcagct ccacatagtt gcgtccccag accaaccata tggcgatgtc ggagctccga
      301 atgatagttt ggtatattgt tttacccaag ccttgcaaag cattatgtac taattcttca
      361 caagcttata agttttaatg ttcaattcct gatcgatgta ttgcagaagg tataatccgg
      421 aagcaatatt atttgcattt atcattgttg ttgattcata actttgttga cgaattattg
      481 ctcttcctgt tatgtcataa attattatag atccatcagc tgttgttccg ctaaggttta
      541 atgaatttgt aaaaggattt ggaaaaatca aatcattgtc atttaaatca atttcctttg
      601 ctatttcata tttaaaaatt gtgcctcttc tatattctcc tccctgatat gtcattccgt
      661 aaaacgtaga attgatggta attaattctc ctttaggatc acttcccgtt gtctcattga
      721 aattcaccaa tactgaatag ccggtgccat ctgtatttaa tttaaagagc actccgcatt
      781 caaacgaaca attatttgat gttccgcccc aatgtgtaag tccataaagt tttcccctgt
      841 aaaaaattaa atttccttca ggatggctgc catggttgtc atcaaaatcg tgaataatgg
      901 taaattcact gccatcagat ttaattttaa atattgcccc aaaaccgtaa ttaccaccat
      961 tataagtcat gccatacata aaattcccgt cagaaagcaa attgccccaa ggatttcttc
     1021 ctgtcaatcc atcaaatgag tatagacttg taaattctgt tccatcaggc tttactttaa
     1081 aaatggttcc tttatcaaaa attcctcctc cgtatgtagt gccatataaa aatacactat
     1141 ctgagatcaa agtgccatat ggaccgttac cagtaactga accagtgaaa tcatgcaggc
     1201 gcgtataatc tgtgccatcc ggatttattc ggaaaatagt gccgtgatca gtcgctcctc
     1261 caccgagtgt cattccaaat aaataattac catcataata cagctcacct ttaggggccg
     1321 atccgttgtc accatcaaaa tcaagtattt tctcatagtt tgagccatct gtttttattc
     1381 tgaatacaat ccctgaacca tgttcacctc ctttataggt catgccatat aaaaattctc
     1441 ccaccaatat aactgacccg tgagggaatg caccattata ttcatcaaaa tcaagaattt
     1501 tctcatatcc tgaaccatca ggttttattt taaaaataac cccgcaatcc ccggttaaac
     1561 aatttgtacc tgtgcctcca tttgttgtca ttccatataa atatacccca tcataaataa
     1621 gtgagcttga tggatttata ccaaaagaat cattaaaatc aaataattta gtaaactggg
     1681 cctctgcttt gatggtaaat agcaataaaa aaaatgcata ataatatttc atgagcaaat
     1741 gatttaccgt ttgcgattaa gtgtgaagtt acctaatccg aatctacaac aaggtattat
     1801 tatgcatgaa aaattcaaac tttgccatgc attcatcgcg caccactcaa aaacccgggt
     1861 gagtttgggt tgttgttagt ttgttgaagt gtttgagaaa aaagtatttg ataattatca
     1921 tttacatccg aaacccacat gcccgtccca tgagggagtt aactcccgaa taacattggg
     1981 atgctggcat ccccgaaaaa aatgttgttt taaaacgtta atattattta tgtgtttagt
     2041 ttttaacacg caagcgcaat cccctcatca caacaagcag tcattcccac ttgccttggg
     2101 agttaactcc ctaggaggac atccgggagt ttagtttatt tgtgtcagga aatttgcagc
     2161 ttcgtgggag tcatgtttgt tattttgtaa ggacgcatgc aattcgtccc tacaaaataa
     2221 caagcatgac aattattaga taataattaa ttcacgaaag gtatttatta cgtgttgaaa
     2281 cagtttctca aaattgagca cttggttgtg atttgtaggg gcgaattgca tccgcccata
     2341 taatcacaac caagtgcgat gggttggatt aataaaattc agatactgca actcactcc
//
LOCUS       DAORKW010000560         2382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2678_length_2382_cov_3.822518, whole genome shotgun sequence.
ACCESSION   DAORKW010000560 DAORKW010000000
VERSION     DAORKW010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2678_length_2382_cov_3.822518"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLB46_15495"
     CDS             complement(<1..278)
                     /locus_tag="PLB46_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF77983.1"
                     /translation="MKKLLYFLLLLLPMQLMQAQLFTVFESETDDLVNAPGAASGAYN
                     GCSFVDYDNDGWLDLFWVRGGLFRNNGLGGFEKITTSGLRTDAGFGNT"
     gene            complement(354..1568)
                     /locus_tag="PLB46_15500"
     CDS             complement(354..1568)
                     /locus_tag="PLB46_15500"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRF77984.1"
                     /translation="MTEQASFDTWTIIFLLAAVHGFVIAGLLFFHKRGNIHANNILAL
                     IMLLFAISMVYYVLYWTGYAYVFTWTNNWVDPIPYLYGPLLYYYLVTLNENKQQFKLK
                     WHLFPFVLHCLFKIPFIIREIFNKPEWLREYFFKPLMPYLNDINVIFIIGQCVSLSVY
                     AILMYQLVQHNKKTITTFAIPEEITTFKWLRNTWIFFVIFSIGTISYWVLVFVGWLKI
                     EYDYAISVIMTAFIYLVGYYGFRQPSLFSDRIVMQDTAVSEPVPPTPIYTLTTSESGY
                     KKYKNSSLKAETALALKTRLLELMHTEKWYCDNTLKIQDLAVKLEVSTHHLSQVINEL
                     CGQTWSDFIMEFRIEAAKQLLADPNYGDKILAIAFDCGFNNKATFNAAFKKMTGLSPS
                     SYRKQATTKATG"
     gene            complement(1686..>2382)
                     /locus_tag="PLB46_15505"
     CDS             complement(1686..>2382)
                     /locus_tag="PLB46_15505"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRF77985.1"
                     /translation="LVIITLPFFFIFSAIFKDKALIYVMTLCRIISFGLMAFCGIIYR
                     FHTDKRIDKKRTYVLIANHRSNLDAPVAAYSWGGKVRYLAKKELLKIPLLSAIFKVTT
                     VNVDRSSREDRRKSVDRLKKYLSERDNIFIFPEGTRNKTENDPLIPFKDGAFSIAIQT
                     QTPILPMVYYNTDNLMPNKLPLMRPGIIDIYQYPPFEVAGLTEADLPALKQQIHDFME
                     AKYLEVSGKNTNS"
BASE COUNT          790 a          438 c          401 g          753 t
ORIGIN      
        1 catgtgttgc caaatccggc atcagttctc aaaccgcttg tagtaatttt ttcaaagcca
       61 ccgaggccat tatttctgaa taaaccacct ctcacccaaa acaagtcgag ccaaccatca
      121 ttgtcataat caacaaaaga gcaaccatta taagcaccac tcgctgctcc aggagcattt
      181 actaaatcat ctgtttcact ttcgaaaaca gtaaataatt gtgcctgcat cagttgcata
      241 ggcagcaaca aaagcaaaaa gtaaagtaat tttttcatac atcacaaatt ttgctcaaaa
      301 ttgtgctgca ctagcggtta aaaggttcta tcctatcgga tttgacctct tttttatccg
      361 gttgcttttg tagtagcttg tttgcgatag gaagatggtg acaaaccggt cattttttta
      421 aatgctgcat taaatgtggc cttattgtta aatccacaat caaaagcaat agctaaaatt
      481 ttatcgccat aatttggatc tgctaataat tgtttggccg cttcaatccg gaattccatg
      541 ataaaatcac tccaggtttg accgcaaagt tcattgatta cctgagaaag atgatgcgta
      601 gacacttcca gcttgacagc aaggtcctga atttttaagg tgttatcgca gtaccatttt
      661 tctgtatgca ttaattcaag taaccttgtt ttgagtgcca aagcagtttc tgcttttaag
      721 gaagagtttt tatacttttt atacccagat tctgatgtgg ttagcgtgta aattggtgtt
      781 ggtggaaccg gctctgatac agctgtatcc tgcatcacaa ttcggtcaga aaacaacgat
      841 ggttgccgaa agccataata tcctactagg taaataaaag cagtcattat aacagaaata
      901 gcataatcat attcaatttt taaccatccc acaaaaacca gtacccaata actaattgtt
      961 ccaatactaa aaatcacaaa aaatatccat gtattacgca accatttaaa tgtggttatt
     1021 tcttcaggaa tggcaaatgt ggtaatggtt tttttgttgt gctgaactaa ttggtacata
     1081 agtattgcat acacacttaa actaacacat tgacctatta taaaaattac gttaatgtcg
     1141 tttaaatatg gcataagtgg tttaaaaaaa tattccctta accattcagg tttgttaaat
     1201 atttctcgaa taataaatgg aattttaaat aagcaatgta atacaaaagg gaaaagatgc
     1261 catttgagtt taaattgctg tttattttca tttaacgtaa cgagataata atacaatagc
     1321 ggcccgtata aataaggaat gggatcaacc caattatttg tccaggtaaa aacataagca
     1381 tatccggtcc agtatagcac ataatacacc atactgattg cgaaaagcaa catgattaaa
     1441 gccaaaatat tattggcgtg aatattcccg cgtttatgaa aaaagagtaa ccctgctata
     1501 acaaatccat gtacagctgc cagcaaaaaa ataatagtcc aagtgtcgaa cgaagcttgt
     1561 tcggtcatgt tattgaatta ggttacccaa aaacccgcag catgtaagca atacaaatct
     1621 aaacaaaata acaggcatgt gaaatgctga ggttgttttt acagaagtat tacaaaacct
     1681 gtaacttagg aatttgtatt tttcccactt acttcaagat atttagcttc cataaaatcg
     1741 tggatttgtt gttttaatgc aggaagatct gcttcggtta aacctgccac ttcaaacggt
     1801 ggatattggt atatatcgat aatccccggc cgcattaaag gtaatttgtt cggcatcaaa
     1861 ttatcggtat tgtaatacac cattggcaaa attggtgttt gtgtttgaat ggcaatacta
     1921 aatgcgccat ctttaaatgg tattaaaggg tcattttccg ttttatttct ggtgccttcg
     1981 gggaaaataa aaatattgtc tctttctgaa aggtattttt taagtctgtc cacacttttt
     2041 ctcctatcct ctctgctcga tctgtctaca tttacagtag tcactttgaa aatggcactt
     2101 aataatggaa ttttcaataa ttctttttta gcgagataac gcactttgcc tccccaggaa
     2161 taggccgcaa ccggtgcatc tagattagac ctgtgattag caattaatac atacgttctt
     2221 tttttatcga tgcgtttatc ggtgtgaaaa cggtaaatta ttccgcaaaa agccatcaat
     2281 ccaaaagaaa ttattctgca taaggtcatt acataaatta atgccttatc cttaaaaatg
     2341 gcagaaaaaa taaaaaagaa tggcaacgtt ataattacaa gt
//
LOCUS       DAORKW010000561         2371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2702_length_2371_cov_4.418826, whole genome shotgun sequence.
ACCESSION   DAORKW010000561 DAORKW010000000
VERSION     DAORKW010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2702_length_2371_cov_4.418826"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..871)
                     /locus_tag="PLB46_15510"
     CDS             complement(167..871)
                     /locus_tag="PLB46_15510"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77986.1"
                     /translation="MVLMAQMGSFVPAADAKIGIIDKIFTRVGASDNISSGESTFMVE
                     MNETASIMNNISNRSLILLDEIGRGTSTYDGISIAWSLCEFLHQHPTARAKTLFATHY
                     HELIELESKFERIRNYHVSTKEAGNKVIFLRKLQQGGSKHSFGIHVAKMAGMPPGIVN
                     RANEILKQLEDKHIGVDLKDKLKKIQHQNFQLNMFSMDDPQLIKIRELLENIDINSLS
                     PIEALMKLKELQDALM"
     gene            complement(881..>2371)
                     /gene="mutS"
                     /locus_tag="PLB46_15515"
     CDS             complement(881..>2371)
                     /gene="mutS"
                     /locus_tag="PLB46_15515"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRF77987.1"
                     /translation="YFGDKSSAGMSFLDISTGEFFVAQGSAEHFEKLLHSLQPAEIIL
                     SKQHQKQFKERYGNKFYVYAIDDWIYTEDYTREKLLTHFGTVNLKGFGIDDMHQAIIA
                     AGASIHYLSDTEHNNIGHITGISRLAEDAYVWLDRFTIRNLELIHASSEDGSSLLKIL
                     DHTITPMGTRLLKKYIVMPLKDKLAIDARLSMVEELILNTTISTTITNALKQIGDLER
                     VVSKVSLQKINPREVLQLNRALHALVTLKQELIASSNKQLQQLGDQIHLCEIIRKHID
                     EQIAEDAPALLNKGGLIKTGFNAELDELRAIGNTGKDFLLKLQQDEIIRTGIPSLKIG
                     FNNVFGYYLEVTNKYKDVVPSDWIRKQTLTSGERFITPLLKEYEDKILGAEEKILTLE
                     QKLYESLIASLQEYVKPIQLNASVVARLDVLLNFANIATQYHYRRPEITEDYAIHITD
                     GRHPVIEHHLPVGENYVPNSIQLDTDEQQIMIITGPNMAGKSALLR"
BASE COUNT          692 a          478 c          405 g          796 t
ORIGIN      
        1 tttaaaattc gaagctgtac tgtaaaatta tttactaaac cccatttgct aattatcaaa
       61 tcactatcca tccagtggat gtcgttaacc atcccttaag gctccgaatt acattccaac
      121 ttcgcacccg gctgtgtaat tagcacatta gcacattaac ccatcatcac attaaagcat
      181 cctgcagctc cttcaacttc atcaaagcct caatcgggct taaactattg atatcgatat
      241 tttctaataa ctcacgaatt tttatgagtt gagggtcgtc catgctgaac atatttaatt
      301 ggaaattttg gtgttggatt tttttgagtt tatcctttag atctacacca atatgtttat
      361 cttctaattg ttttaaaatt tcgttggctc tgtttacaat tccgggaggc atcccggcca
      421 tttttgcaac gtgaatacca aaactatgtt tactgccgcc ttgttgtagt ttgcggagaa
      481 atatcacttt atttccagct tctttagtgc tcacatgata attgcggata cgttcaaatt
      541 tactttccag ttcaattaat tcatgataat gggtagcaaa caatgttttc gcacgggcag
      601 tcggatgttg atgtaaaaat tcgcataaac tccaggctat agaaatacca tcatatgtgg
      661 aagtgccacg accaatttca tctaataata ttaaactgcg gtttgaaata ttattcatga
      721 tacttgccgt ttcattcatc tctaccataa aagtagattc gccggatgaa atattatcac
      781 tggcaccaac acgtgtaaat attttatcga taataccaat tttggcatca gctgcgggca
      841 cgaaactccc catttgcgcc attaaaacaa taattgctgt ttagcgtaac aacgcacttt
      901 tccctgccat attcggaccg gtaattatca ttatttgttg ttcatccgta tctagttgaa
      961 tggagttagg cacatagttt tctcccaccg gtaaatgatg ttcgattacg gggtggcgac
     1021 catcggtgat atgaatggca taatcttcag taatttcagg acggcggtaa tgatattgcg
     1081 tagcaatgtt agcgaaattc agcaatacat ctaatctcgc aacaacagat gcatttaatt
     1141 gaatcggttt aacatattcc tgtagactag ctatcagcga ttcatataat ttttgttcca
     1201 gcgtaagtat cttttcttcc gcacctaaaa ttttatcctc gtattctttt aaaagcgggg
     1261 taataaatcg ttcgccacta gtaagcgttt gtttgcgtat ccaatccgaa ggtaccacat
     1321 ctttatattt gttggtcacc tccaaataat agccaaacac attattaaac ccgattttca
     1381 aacttggaat accggtgcga ataatttcat cctgctgtaa tttcaataag aaatcttttc
     1441 cggtatttcc aattgcgcgt aattcatcca attcggcatt aaatccggtt ttaataagtc
     1501 cgcctttgtt taataaagca ggggcatctt ccgcaatttg ttcatcaata tgtttgcgaa
     1561 taatttcaca taaatgaatt tgatcaccta attgctgtaa ttgtttgttg ctagaggcaa
     1621 ttaattcctg tttcaatgtt actaaagcgt gcaatgccct gttgagttgc aaaacttcac
     1681 gtggattaat tttttgcaac gacactttgg aaacaacacg ctctaaatca ccgatttgtt
     1741 ttagtgcgtt ggtgatagta gtgctaattg ttgtatttaa aattaattct tccaccatcg
     1801 ataaccgtgc atcaattgcc agcttatctt ttaatggcat cacgatatat ttttttaaca
     1861 atcgtgttcc cattggtgtt atggtatggt ctaaaatttt taataaacta gagccatcct
     1921 cgctagaagc atgaattaat tccagattgc gaatggtaaa ccggtcgagc cacacgtaag
     1981 catcttctgc aaggcgggaa atgccggtaa tatgtccgat gttattatgt tcggtatcac
     2041 tcaggtaatg aatactagca cctgccgcaa taatagcctg atgcatatca tcaataccaa
     2101 aacctttcag atttacggtg ccgaaatgtg tcaacaattt ttcacgcgta taatcttccg
     2161 tataaatcca atcatcaatg gcatacacat aaaatttgtt gccatatctt tctttaaatt
     2221 gtttttgatg ttgtttcgat aaaataattt cagcgggttg taagctgtgt aataattttt
     2281 cgaaatgttc agcacttccc tgtgcgacaa aaaattcacc ggtcgaaata tcgagaaacg
     2341 acatcccggc agaggattta tcgccaaaat a
//
LOCUS       DAORKW010000562         2365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2718_length_2365_cov_3.544589, whole genome shotgun sequence.
ACCESSION   DAORKW010000562 DAORKW010000000
VERSION     DAORKW010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2718_length_2365_cov_3.544589"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLB46_15520"
     CDS             complement(<1..277)
                     /locus_tag="PLB46_15520"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRF77988.1"
                     /translation="MQSAYFENQQYQNENYGVTPLLLGNYENCIFNNCNFSDYDISGF
                     TFIECHFIECNLSLVHLNKTAFRQVKFTNCKMLGLHFENCNTFSLEME"
     gene            complement(384..2024)
                     /locus_tag="PLB46_15525"
     CDS             complement(384..2024)
                     /locus_tag="PLB46_15525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF77989.1"
                     /translation="MKKSYLLLTFALASVFSVSKAQVYAYLDDPTGAYAGVATNATGT
                     NLSRENCLLADPACGVGYSSNKHTTSGYTNGRPSVQFSVTPDAGYQLDVTSISVDIRR
                     NPKGPEFWRIAYSTDGGASWTNSGTDFSVAASGCFTNTNLTWDVADFSTTSTLTVRII
                     GHTAYSSLNGISTLKNIDVAGTVSFADADGDGYASNVDCNDADAAINPGATEICDGID
                     NNCDGNIDEAGGATWYADADGDTYGDAASTTVSCDVPVGYVADNTDCNDGDAAINPAA
                     TEVCDGVDNNCDGNIDEGIDLSISITPSGVIELCKPDDVTLSAVGTFDSYQWYKNGAP
                     LTGETGATYTTNKPAYYQVEGFIGTCSSGLSEVQAVAVYESPNANITYPDGLNLCFAD
                     PLLLKASYDATYTYVWYLDGIEIVGATIANYEATATGDYYCVITSVDGCVRTTATVTV
                     INECREGVAANAAIAVYPNPANDVLAINLTGLESNATANISITDITGKVVYTTAIATG
                     NGSVVANVSLDNNVVSGLYFVTAEVNGTSMKQQFVVIK"
BASE COUNT          796 a          448 c          384 g          737 t
ORIGIN      
        1 attccatttc taatgaaaat gtattgcagt tttcaaagtg gagtcccaac atcttacaat
       61 ttgtaaattt gacttgacgg aaagctgttt tgtttaaatg taccagactt aaattacatt
      121 caataaagtg acattcaata aaggtgaatc cggagatatc ataatcacta aaattacagt
      181 tattaaaaat acaattttcg taattcccta atagaagggg agtgacacca taattttcat
      241 tctggtattg ctggttttca aaataagcag actgcatagt attggtaaat caattgtgta
      301 acaaaggtac cgctattggt tgaaacaaaa caaaaaagcc tctcgattaa cgagaggctt
      361 ttaaaatgta aacagtaaat tatttattta attacaacaa actgttgttt cattgaagtt
      421 ccattaactt cagcagtaac aaagtataaa cctgaaacaa cattgttatc aaggctcaca
      481 ttggctacta cactgccgtt tccggttgcg attgcagttg tataaactac tttaccggta
      541 atatcagtga tgctaatatt tgcagttgca ttactttcca aaccggttaa attaattgct
      601 aatacatcat tagcagggtt aggatatact gcaattgcag catttgcagc aacaccttca
      661 cggcattcgt taattacggt aactgttgca gttgttctaa cacaaccatc aacacttgta
      721 attacgcaat aatagtcgcc ggttgcagta gcttcataat tagcaattgt agcaccaaca
      781 atttcaatac catctaaata ccatacataa gtgtatgttg catcgtaact agcttttaat
      841 aatagtggat cggcaaaaca taaatttaat ccatcaggat aagtaatgtt agcatttggg
      901 ctttcataaa cagcaactgc ctgaacttct gataagcctg aagaacaagt gccaataaat
      961 ccttcaacct gataataagc aggtttgttt gtagtgtaag tagcacctgt ttcacctgtt
     1021 aaaggagcac catttttata ccactgataa ctatcaaatg taccaacagc agataatgtt
     1081 acatcatcag gtttacataa ttcaataaca cctgaaggtg taatactgat tgataaatca
     1141 atgccttcat caatattacc atcacaattg ttatcaacac catcgcaaac ttcggttgca
     1201 gcagggttaa tagcagcatc gccatcatta caatcggtat tatctgcaac ataacctaca
     1261 ggaacgtcgc aagatacagt tgtagaagca gcgtcaccat aagtgtcacc atcagcatct
     1321 gcataccaag ttgcaccacc ggcttcatca atattaccat cacaattgtt atcaataccg
     1381 tcacaaattt cagttgcacc cggattaata gcagcatctg catcattaca atctacgtta
     1441 gaagcatagc catcaccatc agcatcagca aatgaaactg ttccggctac atcaatattt
     1501 tttagtgtag aaataccgtt taatgaacta tatgcggtat gaccaatgat acgaacggtt
     1561 aaagtagaag tagttgaaaa atcagcaaca tcccaagtta aattagtatt ggtaaagcaa
     1621 ccgctggccg caactgaaaa atctgtcccg ctatttgtcc agctggcacc accatcagtt
     1681 gaataggcta ttctccaaaa ttcaggtcct ttaggatttc ttctgatatc aacagaaata
     1741 ctggtaacat ccaattgata acctgcatca ggtgtaacac taaattgtac actagggcga
     1801 ccgttagtgt aaccagatgt ggtatgttta ttagaactat atcctacacc acatgcagga
     1861 tctgctaata aacaattttc acgtgataaa tttgttccgg ttgcatttgt tgcaacacct
     1921 gcatacgctc cggtcggatc atccagatac gcatacactt gtgctttcga tacagaaaac
     1981 acgctcgcta acgcaaaggt tagtaatagg tagctttttt tcataaaatt gattttttag
     2041 taattagtaa tagtaatcgt gcgggtatat tttcatttaa agacgttgca agttatagca
     2101 ttggctgttt gcttgtcaaa aacgatatta cattttaaag tctcaaattt gcatttattt
     2161 tatttcacaa aagattgata ttcataatat aatatttaaa aaaaatgata tccacatata
     2221 gtttgttcag tcactttatt tacaaaaatg aaaaattatt cgggccgtgt ttaacctgtg
     2281 attagtgctt ttttatactg tttcattggt tttgtgacga aaaatcttaa acaaaaaatg
     2341 aagcatgata catttatgtc acatt
//
LOCUS       DAORKW010000563         2359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2726_length_2359_cov_4.520399, whole genome shotgun sequence.
ACCESSION   DAORKW010000563 DAORKW010000000
VERSION     DAORKW010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2726_length_2359_cov_4.520399"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..918
                     /locus_tag="PLB46_15530"
     CDS             199..918
                     /locus_tag="PLB46_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77990.1"
                     /translation="MKSQINTHLGKVMSCFLLLSLWFVFLTSFISIRPMDNVTVRGYL
                     PSFCPPDSTIDTSSTFYEFGDTIAVNLDGGDTIFIINDIIDTAMANGRIIGTGDIIVA
                     LTNHQNELPDEMYGVNLADFFMPGHASDSDNVEYATGTNPWELLAEMAPNVLRYPGGS
                     SSKFRHAFGSMNTNNGTPYALLKNGGNGISLEEIIEFYDKTALPFASPTCLEVYNDYE
                     ADTILDGTWLAANQKENFIDF"
     gene            1078..>2359
                     /locus_tag="PLB46_15535"
     CDS             1078..>2359
                     /locus_tag="PLB46_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77991.1"
                     /translation="MSEPVMQVINLINYLQDEELNNEYSVSVYGLELGNECYFDIFDD
                     LIGFGCYDGNSAFEHYWEYVNGTEDYDSAFATSGDFDLEQVMTVAGMTGMEGGFQKHD
                     YIGAIRADETLDNIKIGIPVRNPDVDTGPFIVTPQDDLVLGAIAGGSCTSWNEDVYSK
                     YGALESGKSAFDAIIPHQYYTSQNDGVPDSNTNWGNIPLGLDDPLVEGDNCLDNDTDD
                     EFHDNFTTIYTYATEDTRLECAFRGIVGTSGYFTVGSFNEFIKTRHKEGMVDLSGELS
                     LGESETNPKETWITEWNIKNNNSFDDTDAVNKDIRLEVYNNTIAHAYIIQEQYLNTLK
                     FNNIKNETFRENFITVTTLQNYLGGSSIQLVTKAKKQDMVALGLSGSCSGIGFKNYVP
                     RTLYHSYKLLNQLNLNNLNYVKSNKTIYASNINQP"
BASE COUNT          815 a          360 c          436 g          748 t
ORIGIN      
        1 gatgataaat aggggcaggt actcagtaaa acaatacata tgtacttttt aaatcgcaaa
       61 ataagttatt caaaattacc aaaaaattga tatatgttgt atctaattga tattcagtcg
      121 tttatgaaat aaatgcaatt agaatcaaaa tataattttt actatatttg ttaatctttc
      181 acttttaaac ccaattgtat gaaatcccaa atcaataccc atttaggcaa agtaatgtct
      241 tgttttttgc ttttatcatt atggtttgtt tttttgacaa gttttatttc tataagacct
      301 atggacaatg taactgtgcg cggttatttg ccaagttttt gtcctcctga ctcgactatc
      361 gacacttcat caacatttta tgaatttggc gatactattg cagttaattt agatggcggt
      421 gataccatct ttataatcaa tgacattatt gatacagcaa tggcaaatgg gagaataatt
      481 ggtacgggtg acataattgt tgcacttact aatcaccaaa atgaattacc agatgaaatg
      541 tatggagtaa accttgcaga tttttttatg cctgggcatg cttcagattc tgataatgta
      601 gaatatgcta ctggaacgaa cccctgggaa ctactagctg aaatggcccc aaacgtcctt
      661 cgatatcctg gcggctcatc aagtaaattt agacatgctt ttggttcaat gaatacgaat
      721 aatggaactc catatgcctt actcaaaaat ggtggaaatg gcattagttt ggaagaaata
      781 attgagttct atgacaagac tgcactgcct tttgcaagtc cgacttgctt agaagtatat
      841 aatgattatg aagcagatac tattttggat ggtacttggc tagcagccaa tcaaaaagaa
      901 aatttcattg atttttaaaa cgaatggaaa aaccaacctc actataatcc aagtgataca
      961 gcttacgacg aaatatacaa agaaccttta tatataaacc aacttataga cttagttgaa
     1021 caaatagaaa ccgctaatcc agggttaacg gtcaagataa tgtatgttgt aaatattatg
     1081 agtgaacctg ttatgcaggt tattaattta attaattatt tacaagatga agaattaaat
     1141 aatgaataca gcgtaagtgt atatggttta gaacttggga atgaatgtta tttcgacatc
     1201 ttcgatgatt taattggatt tggatgttat gacggcaatt ccgcatttga acattactgg
     1261 gagtatgtta atggaactga agattacgat tcagcatttg cgacatctgg tgattttgat
     1321 ttagaacaag ttatgacggt ggcgggtatg acaggaatgg aaggcggatt tcaaaagcat
     1381 gattatatag gagcgattcg cgcagacgaa acattagaca atattaaaat tggtatacca
     1441 gtaagaaacc cagatgttga tactgggccg ttcattgtca caccacaaga tgatttagtt
     1501 ttgggcgcta ttgctggtgg ctcctgcaca tcgtggaatg aagatgtata tagtaagtat
     1561 ggtgctttag aatcaggaaa atctgcattt gatgccatca tcccacatca atattatact
     1621 tcccaaaatg atggtgttcc agattctaac actaactggg gcaatattcc actaggccta
     1681 gatgatcccc ttgtagaggg tgataactgt ttagataatg atacagatga tgaatttcat
     1741 gataatttta caactattta tacttatgct acggaagata cccgtcttga atgtgctttt
     1801 cgaggcatag ttgggacaag tggctacttt actgtaggaa gttttaatga atttattaaa
     1861 actagacaca aagaagggat ggttgactta tcgggagagc tatctcttgg agaaagcgaa
     1921 acaaatccaa aagaaacatg gattactgag tggaatatta aaaataacaa ttcctttgat
     1981 gatactgatg ccgtaaataa agatatacga cttgaagtat ataacaatac tattgcgcat
     2041 gcttatataa ttcaagagca atatttaaac acattaaaat ttaataatat taagaatgaa
     2101 acattccgag aaaattttat aacagtaacc acacttcaaa attatctagg tggtagctca
     2161 atccaattgg tgacgaaagc gaaaaaacaa gatatggtag cacttggttt aagtggttct
     2221 tgctccggaa ttggttttaa aaattatgtg ccacggacgt tgtatcattc ctataaattg
     2281 ctaaatcaac ttaatttaaa caatttaaat tatgtaaaaa gtaataaaac aatttatgcc
     2341 tctaatatca atcaaccac
//
LOCUS       DAORKW010000564         2358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2729_length_2358_cov_3.864959, whole genome shotgun sequence.
ACCESSION   DAORKW010000564 DAORKW010000000
VERSION     DAORKW010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2729_length_2358_cov_3.864959"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLB46_15540"
     CDS             complement(<1..297)
                     /locus_tag="PLB46_15540"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77992.1"
                     /translation="MKKTLFVCMVLITVVFAACKSTKTGDEQVYSPFDSTSLDSTVNP
                     GDDFFAWVNNKWLEANPIPADKASFGSFHMLDDNSLTTLRVSMEKAAGANAAKGT"
     gene            512..820
                     /locus_tag="PLB46_15545"
     CDS             512..820
                     /locus_tag="PLB46_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF77993.1"
                     /translation="MLLIIGVINSKEIMHSFVWHDAIGCTAHYDEHGHLTHHEGVAAT
                     GDYCAFCHLDFSIFSTPEEQTLTNSSFVYQTLTITKPVAPHLPAATGALLLRGPPAIA
                     "
     gene            887..>2358
                     /locus_tag="PLB46_15550"
     CDS             887..>2358
                     /locus_tag="PLB46_15550"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF77994.1"
                     /translation="MRKIWVFIFSFIVLQFHVAAQQQGCNLQIGGRVTDKEHEALLSY
                     ATVYILELQSGAVCDDNALFAIGELCAGNYTLLISHLGCATDTVTIQLEKSVFLEFEL
                     NHQDSVLEAIQVHVSMETYKGTLTTDYIEGKALEAIAGKSLGNALKDMNGLNTIQTGN
                     SISKPVIHGLHSNRILIMNNGVRQEGQQWGNEHGPEIDPFIADNITVIKGAAGVKYGS
                     DAMGGVILVNPAPLIDTGGTRGALQLAGFSNTKGGAASAYIQSHYAPFAWRLQGTLKA
                     EGTAQTPDYYLANTAFREANGSAAVKYHKNDFETELYYSYFHNTIGIFSASHIGNLTD
                     LENAFISDTPYIAGNFTYEIGRPYQLINHHLVKSVSHYHNDKFGNIYLTAAWQNNIRK
                     EYDTHVPLDDSIAALGLPSLYFEIGTITTELEWKHHKKNNFSGSVGLSGMNQQNITRY
                     STFIPNFKNYSGGIYALETYQLIDWSLEAGLRYDYKWTKA"
BASE COUNT          725 a          425 c          468 g          740 t
ORIGIN      
        1 agtgccttta gcggcattag cgccggcagc tttttccatt gaaacacgca aagttgttaa
       61 actattgtcg tctaacatgt ggaaactgcc aaagctggct ttgtctgccg gaatcggatt
      121 agcttccagc catttattat tcacccaggc aaaaaagtca tcaccggggt tcaccgtact
      181 atctaacgat gtgctgtcga atgggctata aacttgctca tcgccggttt tagtgctttt
      241 acaggctgca aatactacag taataagcac catgcataca aataatgttt ttttcattgt
      301 aaggtttggt tttacggcgc aaagataacc ttttaacctg cgaaaaagca ggatgactgg
      361 ttaaaacagg ttaaaggatg ttaagaaaag cccgttttaa taaggtttca gctcaagtgg
      421 tcagcaattt gattgtgatt ttaacaaaat gcgtattttt gctaagatgt taaaccgtgt
      481 aaaaagggta aacctgattg caatttattt catgttattg ataattggtg ttatcaatag
      541 caaagaaatc atgcacagtt ttgtctggca cgatgctatc ggatgcactg cacattacga
      601 tgaacatgga catttaacac atcatgaagg tgttgcagca acgggtgact attgcgcgtt
      661 ttgccacctc gatttttcca ttttttccac tcctgaggaa caaactttaa ccaattcatc
      721 atttgtttat caaacactaa ccattacaaa acccgttgca ccgcatttac ctgcagcaac
      781 aggtgcattg ttacttcgcg gccctcccgc aatcgcataa tttttagatg attgttttat
      841 tatttgcctg catttatggt gccgggcaat ttaattattt gaatgtatgc gaaagatttg
      901 ggtatttata ttttctttta ttgtattaca atttcacgtt gccgcgcagc aacagggatg
      961 taatttgcaa attggtggta gggtgaccga caaagaacat gaagcgttgt tgtcgtatgc
     1021 tacagtttat attttggaat tacaatcggg tgcagtttgt gatgacaatg ccttatttgc
     1081 cattggggag ttatgcgcag gcaattacac attattaatt tctcacttag gttgtgctac
     1141 cgataccgtt actatacaat tagaaaaatc ggtatttctg gagtttgaat taaatcatca
     1201 ggattcggtt cttgaagcca ttcaagtgca tgtgagtatg gaaacatata aaggcacact
     1261 taccaccgat tatatagaag gtaaagcatt ggaagccatc gcagggaaat cgctgggtaa
     1321 tgcgttaaaa gatatgaatg gattaaatac cattcaaaca ggaaacagta tttcaaaacc
     1381 ggtaattcat ggtttacata gtaatagaat tttaatcatg aataatggtg ttcgccagga
     1441 agggcaacaa tggggcaacg aacatggtcc ggaaatagac ccttttattg cagataatat
     1501 tacggtaata aaaggtgcag caggtgttaa atacggaagc gatgcaatgg gtggtgttat
     1561 tttagttaac ccagctccct taattgacac aggtggcacg cgaggtgcgc tgcaacttgc
     1621 cggtttttcc aataccaaag gcggagcagc ttctgcctac attcaatcac attatgcacc
     1681 ttttgcctgg cggttacaag gcacacttaa agctgaaggt actgcccaaa cacctgatta
     1741 ttatttagcc aatactgctt ttcgcgaagc aaatggttca gctgcggtta agtatcataa
     1801 aaatgatttt gaaacagaat tatattatag ttattttcat aacactattg gtatattttc
     1861 tgcatcacat attggtaatt taaccgattt agaaaatgca tttatttcag acacgccata
     1921 tattgccggt aattttactt acgaaattgg taggccatac caattaatta atcaccattt
     1981 agtaaaatcg gtttcgcatt atcacaatga taaatttggt aatatatact taactgctgc
     2041 atggcaaaat aatattcgta aagaatatga tacacatgtt ccgcttgatg acagtattgc
     2101 agcattaggc ttaccttctt tatattttga aattggtact attaccacag aattagaatg
     2161 gaaacatcat aaaaaaaata atttctccgg atctgttgga ttaagcggta tgaaccaaca
     2221 aaacattacg cgatacagta catttattcc caatttcaaa aattactccg gtggtattta
     2281 tgcgttagaa acctatcagt taattgactg gtcgcttgaa gcaggtttac gatatgatta
     2341 taaatggaca aaagcgtt
//
LOCUS       DAORKW010000565         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2732_length_2356_cov_4.901782, whole genome shotgun sequence.
ACCESSION   DAORKW010000565 DAORKW010000000
VERSION     DAORKW010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2732_length_2356_cov_4.901782"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..822)
                     /locus_tag="PLB46_15555"
     CDS             complement(190..822)
                     /locus_tag="PLB46_15555"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF77995.1"
                     /translation="MNAQEYETRFSLNPYHFGIAIAYNTSDLRVTFSDQFINHDSVMV
                     VESTNGPGFNLGIITNMRLGKYFDLRFVPSLSFAEKKLNYTYRDEGTSFQTLESLYLD
                     LPFDIKFKSAAYKDMKVYVLAGVKYAYDFSSNADARNAEGLVKINANDLAINCGFGFE
                     FYFPYFIFSPEIKLSNGILNLHATDPLLQESYVMDKLFSRTLLFTIHIEG"
     gene            complement(867..1592)
                     /gene="ubiE"
                     /locus_tag="PLB46_15560"
     CDS             complement(867..1592)
                     /gene="ubiE"
                     /locus_tag="PLB46_15560"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRF77996.1"
                     /translation="MSESVTPYKSDSSKKEQVKTMFNNIAGRYDFLNRVLSMRVDVYW
                     RNRAIKRLKKYQPKTILDVACGTGDFTIAALKTGATKIIGVDISVQMLEAGIKKINNK
                     KLADKITLTEGDSENLQFEKNTFDACTVAFGVRNFENLQKGLDEIYRVLNTGAPIVVL
                     EFSKPKSFPFKQLYNFYFSTILPAIGRLVSGDSRAYTYLFESVSAFPEGDAFIQYLKQ
                     AGFKNCTCTRLSLGICSLYIAEK"
     gene            1664..2278
                     /gene="yihA"
                     /locus_tag="PLB46_15565"
     CDS             1664..2278
                     /gene="yihA"
                     /locus_tag="PLB46_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRF77997.1"
                     /translation="MKSYFCAVIFKTAVYTGSFPDYLKCPEKDLPEFAFIGRSNVGKS
                     SLINMLTNHQHLAKTSARPGKTQTINFFLINNTWNLVDLPGYGFAKVSKNQREQFEKM
                     ISDYLKYRPQMLCVFQLVDACIPPQKIDLEFTDWLGEEHIPFCIAFTKTDRRKKDIKG
                     NIEAYKQKLAESWETLPACFVTSAETRAGRDEVLAYLDNVLAAR"
BASE COUNT          726 a          456 c          454 g          720 t
ORIGIN      
        1 gagggggaat atataatttt attgtaaaag gaacctgcca ggcggcagac aggcactgat
       61 ttaacagatt aaacggatga aaacggatta attatttcta taaaatgata ttaaaacaac
      121 tcaacaaaaa tccgtcccta tcggttcaaa ccgtttaatc tgtgcctgtc tgccgccagg
      181 caggttccat tacccttcaa tgtgtatcgt aaacaacaag gtcctgctaa acaatttatc
      241 cattacataa ctttcttgca acaacggatc ggttgcatgt aaattgagaa ttccgttaga
      301 aagttttatt tcaggagaaa aaataaaata agggaaataa aattcaaatc caaaaccgca
      361 attaatggct aaatcgttgg catttatttt taccaatcct tctgcgttgc gtgcatcggc
      421 attggaacta aaatcgtatg catattttac acctgccagc acatatactt tcatgtcctt
      481 gtaggcagca cttttaaatt taatatcaaa tggtaaatcc aaatacaatg attcaagtgt
      541 ttggaacgat gtaccttcat cgcgataagt ataattcagt tttttttcgg cgaaagataa
      601 tgatggcaca aaacgtaaat cgaaatattt acctaaacgc atattggtaa taatacctaa
      661 attaaatccg gggccatttg tggactccac caccataact gaatcgtgat taataaattg
      721 atcgctgaat gttacacgca ggtcggaagt attataagca atggcaatcc cgaaatgata
      781 tggatttaac gaaaaacgcg tttcgtattc ctgtgcattc atttgggctt ttaccattaa
      841 aggcaaaaaa catatgcata ttaaaattac ttttctgcaa tatacagcga acatattcct
      901 aaacttaatc ttgtgcaagt gcaattttta aatcctgcct gttttaaata ttgtataaag
      961 gcatcgcctt ccggaaatgc tgaaactgat tcaaataaat aggtgtatgc tcgtgagtct
     1021 ccactcacta aacgtccaat tgccggaagt attgtactga aataaaaatt atacaattgc
     1081 ttaaacggaa atgatttggg ttttgaaaat tccaacacta caattggtgc acctgtattt
     1141 aatacccgat aaatttcatc caatcctttt tgcagatttt cgaaattgcg cacgccaaaa
     1201 gcaaccgtgc aggcatcgaa agtatttttt tcaaactgca gattttcaga atcaccttct
     1261 gtaagtgtta ttttatctgc taatttttta ttatttattt ttttaatacc tgcctccaac
     1321 atttgcaccg aaatatctac accaataatt ttggtggctc cggtttttaa tgctgcgatg
     1381 gtaaaatcac ccgtaccaca tgccacatcc aaaattgttt tcggctggta ttttttgagt
     1441 cgtttaatgg ctctgttgcg ccaatacaca tccacacgca tcgataacac cctattgaga
     1501 aagtcgtacc tgccggcaat attgttaaac atggttttaa cctgctcctt tttggaggaa
     1561 tcggacttgt aaggtgttac actttcagac atctgggtgc aaatttagca tgaataaata
     1621 tgtttaatat cgaaatgact gttaacttct ccatttcagg cttttgaaat cttatttttg
     1681 tgctgtgata tttaaaacag cagtttacac aggaagtttt cccgattacc tgaaatgtcc
     1741 cgaaaaggat cttccggaat tcgcttttat tggtcgctcc aacgttggca aatcgagttt
     1801 gattaatatg ctgaccaacc atcagcatct ggccaaaacc agtgcgagac cgggaaaaac
     1861 gcaaactatt aacttctttt taatcaataa tacctggaat ttagtcgatt tacccggcta
     1921 tggcttcgca aaggtgtcta aaaaccagcg tgagcagttt gaaaaaatga tcagcgacta
     1981 cctgaaatac cgtccgcaga tgttatgtgt gtttcagttg gtagatgcct gtattccgcc
     2041 ccaaaaaata gaccttgagt ttacggattg gctgggggaa gagcatattc cgttttgtat
     2101 tgccttcacg aaaacggata ggcgaaaaaa ggatattaag ggcaatattg aggcctacaa
     2161 acaaaaacta gccgaaagct gggagacgtt gcctgcatgc ttcgttactt ctgctgagac
     2221 aagggctggt cgggatgaag tgctagccta cctcgataat gtgctggctg cacgatgaga
     2281 ttgtttcacg cggagccgcg gggggatttg gattgtatgg tgtgtttttt tgagagcgcg
     2341 gagctgggaa accagt
//
LOCUS       DAORKW010000566         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2733_length_2356_cov_4.322468, whole genome shotgun sequence.
ACCESSION   DAORKW010000566 DAORKW010000000
VERSION     DAORKW010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2733_length_2356_cov_4.322468"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLB46_15570"
     CDS             <1..769
                     /locus_tag="PLB46_15570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF77998.1"
                     /translation="THPVISYTFYDTITVFPAVTTPEITLSGATTFCSGDSLVLSTTE
                     VAGISYQWSMDGDPIPGADSTSLVVFASGEYALSATGVGGCFAVSSSTAVTVYEQPTP
                     PSIIVIGNTLNTSSTWDVQWYYNGSPIPGATSTTYTPAVEGLYQVAAINGPCVAYSVE
                     INFIFQSVNELLIDKITISPNPNNGAFSATFNVLKNQNISITVQNIIGSTVYHKNYEN
                     ISGAVQENILLPGVNSGVYFLQIKGESGSIIEKIIIQ"
     gene            776..2170
                     /locus_tag="PLB46_15575"
     CDS             776..2170
                     /locus_tag="PLB46_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF77999.1"
                     /translation="MATNFDELVNAQRNFFSSKKTIPYAYRIEQLKKLADLIQRYENQ
                     FVATLYQDLHKSKFEAWGAEVGVVINELNYYINHLKKWMLPSKIKTPLFHFRSTSYIQ
                     QIPYGNTLIIAPWNYPFLLVLRPLIGALAAGNTAIVKPSENAPATAALLEEMINEHFD
                     KSYLHVINTDVAGAQQLIQQKFDYIFFTGGESIGKIIYQEAAKNLTPVTLELGGKNPC
                     IVDETAQLDITAARITWGKFSNCGQTCVAPDYILVHKNVREALIQKIIANIEKSYGKD
                     AQKSPDYGRIVNTFHHQRITKMITPETVIYGGQNDVNDKYFAPTLLNIQDPQHAAIMQ
                     QEIFGPVLPIITYTDINETFDIINQHPNPLVAYLFSNNTELIKKATTEIKSGDMSINE
                     AVIHFGQLMMPVGGVGNSGIGKSQGKSTYLTFSHPKSVMQKKFFPDLPVRYPPYSQKQ
                     LARLKLLFRYLFTR"
BASE COUNT          785 a          441 c          424 g          706 t
ORIGIN      
        1 aacccatcct gttatttcct atacttttta cgatactatt accgtatttc cagcagtaac
       61 tacgccggaa attactttaa gcggagctac cacattttgc agcggcgatt cactggtgtt
      121 aagcactact gaagttgccg gtatcagcta ccaatggtcc atggacggtg accctattcc
      181 gggcgcggac agcacatcat tggtggtatt tgcttcgggc gaatatgctt tatcagctac
      241 cggcgttggc ggatgttttg cagtaagcag ctcaacggca gtaaccgttt acgaacaacc
      301 aacccctcca agtattattg tgattggtaa tacactcaat acatcatcta catgggacgt
      361 gcaatggtat tataatggaa gccctattcc cggcgctaca agcacaacct atacccctgc
      421 ggttgaaggc ctatatcaag ttgctgctat aaacggacct tgtgtagcat attcagttga
      481 aattaatttt attttccagt ccgtgaacga gttacttatt gacaaaatta caataagtcc
      541 caaccctaat aacggtgcat tttctgcgac ttttaatgtg ctaaaaaatc aaaatatcag
      601 tataactgtg caaaatatta taggttctac tgtttatcac aaaaattatg aaaatatatc
      661 cggtgcagtg caagaaaata tattgcttcc gggtgtaaat agtggtgtat attttttaca
      721 aattaaaggc gaatcaggta gtattatcga aaaaataatt attcagtaaa ttttgatggc
      781 gacaaatttt gatgaactgg taaatgcaca acgcaatttt ttttcaagta aaaaaacaat
      841 tccttatgcc taccgaattg aacaattgaa aaaacttgcg gatttaattc agagatacga
      901 aaatcaattt gttgcaacat tatatcagga tttacataaa tcgaaatttg aagcttgggg
      961 tgctgaggtt ggtgtagtaa taaatgaatt gaattattac atcaaccacc tgaaaaagtg
     1021 gatgttacca tccaaaatta aaacaccttt attccatttc aggagcacca gttatattca
     1081 acaaataccc tatggcaata ctttaattat tgcgccatgg aattatcctt ttttattagt
     1141 gttgcgacca ttaattggtg cattagcagc tggcaatacg gcaatcgtaa aaccttcaga
     1201 aaatgcaccg gcaacagcag ctttgttgga agaaatgatt aatgaacatt tcgacaaatc
     1261 atacttgcat gtaattaata ccgatgttgc gggagcacaa caattaattc aacaaaaatt
     1321 tgattacatt tttttcaccg gcggcgaatc aatcgggaaa atcatttatc aggaagcggc
     1381 taaaaacctt actcctgtta cattagagct cggtggtaaa aacccgtgta ttgtagacga
     1441 aacagcacaa ctcgatatta ccgctgccag aattacttgg ggaaaattta gtaattgcgg
     1501 tcaaacttgt gttgcaccgg attatatttt ggtgcacaaa aatgtccgcg aagcgctgat
     1561 tcaaaaaata attgcgaaca tagaaaaatc atacggaaaa gatgcgcaaa aaagtcccga
     1621 ttatggaaga attgtaaaca cttttcatca tcagcgtatt accaaaatga taacacctga
     1681 aactgtgatt tatggtggcc aaaatgatgt taacgataaa tattttgcac ctacattatt
     1741 aaacatacag gacccacaac atgctgccat aatgcaacag gaaatttttg gacccgtttt
     1801 accaattatt acttatactg atattaatga aacatttgat attattaacc aacatcccaa
     1861 tccattagtt gcttatttgt tttctaataa tactgagtta attaaaaaag caactactga
     1921 gattaaaagc ggagatatgt ccatcaacga agcagttatt cattttggtc agctcatgat
     1981 gccggttggt ggtgttggca atagtggcat tggaaaaagt cagggtaaat ctacttacct
     2041 cactttttca catccaaaaa gtgtaatgca aaaaaaattc tttcctgact taccggttcg
     2101 atatccgcct tattctcaaa aacaattagc gcgattaaaa ttattatttc gttatttatt
     2161 tacgagataa aaaaaatgct gctttttgta gccagttttt ataaaggggg tataaattaa
     2221 agctacctcc tacccgtttt cttttcataa cttcgcaact taaaataatt gcatgcagga
     2281 attagcaaaa cgtatcgcca atctgaatga atcagcaacc ataaagatgg cgcagttgac
     2341 gcgggaattg aagagc
//
LOCUS       DAORKW010000567         2355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2735_length_2355_cov_6.399565, whole genome shotgun sequence.
ACCESSION   DAORKW010000567 DAORKW010000000
VERSION     DAORKW010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2735_length_2355_cov_6.399565"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..730
                     /locus_tag="PLB46_15580"
     CDS             161..730
                     /locus_tag="PLB46_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78000.1"
                     /translation="MAIPGEYYLTGVHEMGCGIKLNEDFSFEFFFSYGALDRHGYGTW
                     KSDENNHVILNTNYDNQVPFSITLSEKRAFEGIKMGFPNYNKVLLNETTIKVIANGLE
                     EEQVVGAQGIFHFSSKHADKIIVTCLFYFDNPATLVPADTSHNYFELAANHGLPLVHF
                     KDAIFVADETALEGKLHIFDETKTFRFIK"
     gene            733..1269
                     /locus_tag="PLB46_15585"
     CDS             733..1269
                     /locus_tag="PLB46_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78001.1"
                     /translation="MNNDFEKLHSCITDTETIIQTLNSFNETVHSAGMYFRLDVPDED
                     GGIAYPNNEIPDKAVIVIGSFDFSYYHQIEIVFFEAITHTISSESIWADHWDKEQLTL
                     IQGTTRYDILTKHQPYDDDTFHVFAFNIGSFGDEQYYIIAKGISIKLGIVFHYDRARQ
                     EPLKPDERVAYWVHQSSK"
     gene            complement(1261..2175)
                     /locus_tag="PLB46_15590"
     CDS             complement(1261..2175)
                     /locus_tag="PLB46_15590"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRF78002.1"
                     /translation="MLRTILLLIIALIIVPLVAFYVDDPLTTLQQTMLRTAAFMALGV
                     ALYCFIVSELARNYSQVDKLWSIVPLLYGWYFASASNWEPRIVLMAVLISIWGARLTY
                     NFSRRGAYQWKFWDGEEDYRWAILRQQPHLNSRFKWGLFNLFFISLYQNGLILMFTLP
                     AVMVVGASKGITTVDIVLAIISVGFVLMEFIADQQQWNFQKEKYRRINNNESLTEPYS
                     DGFVSSGLWKYFRHPNYTAEQAIWVVFYLFSVSATGKWLNWSIAGCVLLMLLFQGSAD
                     FSEKISAGKYPKYKDYQKRVGRFFPKLF"
BASE COUNT          794 a          428 c          405 g          728 t
ORIGIN      
        1 cctctatggt ttcggctgtt ttcgcctcgg cggacgcttc gcgaattggt agcaaaataa
       61 tttaaaaatc gctcggcgta attttctgca gatttaaatg cgttcataat ataaacatcc
      121 ctgtataata gtaactttca cccgttttaa tttttctact atggctatac caggcgaata
      181 ttaccttacc ggtgtccacg aaatgggatg cggtattaag ttgaatgagg atttttcatt
      241 tgagttcttt tttagttatg gcgctttaga caggcatggc tatggaacct ggaaatcgga
      301 tgaaaacaat catgttatat taaacacgaa ttatgataat caggtgcctt tttcgataac
      361 actttccgaa aaacgcgcgt ttgaaggtat taaaatggga tttcctaatt ataataaagt
      421 cctcttaaac gaaaccacca ttaaagtaat tgccaatggt ttagaagaag aacaggtggt
      481 tggtgcacag ggaatttttc atttttcttc gaagcatgcg gataaaatta ttgttacctg
      541 tttattttat ttcgataacc cggcaacatt agttcctgca gatacatctc ataattattt
      601 tgaattagca gctaaccacg gcttgccttt agtacatttt aaggatgcta tttttgttgc
      661 agatgaaaca gccctggaag gtaaattgca tatatttgat gaaacaaaaa catttcgttt
      721 tattaaataa agatgaataa cgattttgag aaattacata gttgtataac cgatactgaa
      781 acaattattc agacattaaa cagttttaat gaaacggtac acagcgcagg catgtatttc
      841 aggctagatg ttccggatga ggatggtggt attgcatatc cgaataatga aattccggac
      901 aaagcagtta ttgtaatagg cagctttgat tttagttatt atcatcaaat tgaaattgtt
      961 ttttttgagg ctattacaca taccataagt agtgaaagta tctgggccga tcattgggat
     1021 aaagaacagt tgactttaat tcaaggcaca acccgttatg atatattgac caaacaccaa
     1081 ccgtacgatg atgacacatt tcatgtgttt gccttcaata ttggttcgtt tggcgatgaa
     1141 cagtattata taatcgccaa aggtatttcc attaaactag gtattgtatt tcattacgac
     1201 cgcgctcgac aggagccatt aaagccggat gaacgtgttg cttattgggt gcatcaatcc
     1261 tcaaaataat tttggaaaga aacgaccaac ccgtttttga taatctttat atttaggata
     1321 ttttcctgca gaaatttttt cactgaaatc tgcactaccc tgaaataaca acatcaacaa
     1381 aacacaacct gcaatactcc agttgagcca ttttccggtt gcgctaacac taaataaata
     1441 aaatactacc catatcgctt gttcagcagt ataatttgga tggcggaaat atttccacaa
     1501 ccctgacgat acaaacccat cagaataagg ttcagtaagc gattcattat tattgattct
     1561 gcgatatttt tccttttgaa aattccattg ttgttggtcg gcaataaatt ccatcagcac
     1621 aaatcctact gaaataatag ccaaaacgat atcaacagtt gtaattccct tacttgcgcc
     1681 gacaaccatt actgcgggca aggtaaacat aagaattaag ccattttggt acagggaaat
     1741 aaaaaataaa ttaaacaacc cccatttaaa tcgtgaattt aaatgtggtt gctgacgcaa
     1801 aattgcccag cgataatctt cttcaccatc ccaaaatttc cattggtaag ccccccttct
     1861 gctgaaatta taagtaagtc gcgcacccca gatactaatt aacacagcca ttaaaacaat
     1921 acgcggctcc cagttggatg ccgatgcaaa ataccatcca tataataatg gaacaatact
     1981 ccataattta tctacctgac tataattacg tgctaattcg cttactataa aacaatacaa
     2041 agcaacacca agggccataa atgcagcagt gcgcaacatg gtttgttgta atgtagttaa
     2101 tgggtcatct acataaaatg caactagtgg aactatgatt aatgcaataa ttaaaagaag
     2161 tattgtccga agcatgccaa aaatattttt tgtaaaagaa tcaaataatt tgcaatttaa
     2221 aaaattaaat tatatttgca ttcaaattaa tacaacatgc gtaatttata tcaaataaca
     2281 gaacaaacac caaagggtca gctagatcgt ttgacgtgtc ggggcatgta tttttatgaa
     2341 aaataatatt taaaa
//
LOCUS       DAORKW010000568         2346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2753_length_2346_cov_3.483632, whole genome shotgun sequence.
ACCESSION   DAORKW010000568 DAORKW010000000
VERSION     DAORKW010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2753_length_2346_cov_3.483632"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="PLB46_15595"
     CDS             complement(<1..1480)
                     /locus_tag="PLB46_15595"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRF78003.1"
                     /translation="MFGLRRKWKFGLMVSILLAIAGGITMVSISNCSKANPEHSAKVD
                     SIEIQRLIALDKGTIPVEAFFKNPEKTAFTISPDGDYIAYLGPYNSRLNIYVQPADIT
                     AAPVRVTSGTERDITYYFWKNNTTLLYIKDAAGDENYKLYAIDRDGTNKRDLTPEQNV
                     RIEVIDELTDFPDEVIIAMNKNNPALFEPYRLNIKTGEKKQLAVNKDMSNPITIWKAD
                     NEGKLRLAVSVEKGTKTHLLYRETEDQPFKSLLVSDWKDMVEPLFFDENNKYIYALSN
                     LNRDKTALVKIDPANPQNPEIILEHPDVDITYAERSPKKHKLVSAYYATDKKHLVFFD
                     EDLKKIYEQLALKFPGDDIYFNGFDNEEKHFIVRTYNDKTPGDFYLYHAEENSITHLA
                     TINTNIQKEQMSAMFPIEYKARDGLNIHGYLTVPSGTSGKNLPAVILVHGGPTSRDYW
                     GYRADVQLLASRGYAVLQVNFRGSWGYGKNFTTAGFKQWGKKM"
     gene            complement(1631..2122)
                     /locus_tag="PLB46_15600"
     CDS             complement(1631..2122)
                     /locus_tag="PLB46_15600"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRF78004.1"
                     /translation="MKKIGLLLAIACFGLNNLQAQDALPDLTLYDINGNKVNIADFGK
                     DDKIQVFSFWATWCVPCKEELNNIAEIYEDWQKDYNVELIAVSIDDAKTKANVKAYAE
                     GQGWTYTVLLDTNKDLQRILGGQSVPFTVVTNKAGLITDKHTGYIEGDEYELEDKLVE
                     LSK"
BASE COUNT          703 a          462 c          386 g          795 t
ORIGIN      
        1 gcattttttt gccccactgt ttaaatcctg ccgtggtaaa gtttttacca taaccccagc
       61 tacctcgaaa attcacttgc aaaacggcat aaccgcggct tgccagtaat tgtacatctg
      121 ccctgtaacc ccaataatcg cgacttgttg ggccaccgtg gaccagaatt acggcaggta
      181 aattttttcc ggaagtaccg gaaggaacag ttaaataacc gtgaatattt aaaccgtcgc
      241 gggctttgta ttcaatagga aacatggcac tcatttgttc tttttgaata ttcgtattta
      301 ttgtagcgag atgcgtaata ctattttctt cagcgtgata taaataaaaa tcgccaggtg
      361 ttttatcgtt gtaagttcta acaataaaat gtttttcttc attgtcgaag ccattaaaat
      421 aaatatcatc ccccgggaat tttaatgcca gttgctcata tatttttttc aaatcttcat
      481 caaaaaaaac taaatgtttt ttatctgtag cataatatgc tgaaaccagc ttatgttttt
      541 tgggagaacg ttctgcatag gtaatatcta catccggatg ttcaagaatt atttccgggt
      601 tttgcggatt tgccgggtcg atttttacca gtgcggtttt atctctgttt aaatttgata
      661 atgcataaat gtatttattg ttttcgtcga aaaacagcgg ttctaccata tctttccaat
      721 cgcttaccag caacgattta aatggttggt cttctgtttc tctgtataac aaatgcgttt
      781 tagtaccttt ttcaacactc acagcaagac gcaatttacc ttcattatcc gctttccaaa
      841 tggttatcgg attcgacata tccttattga ctgcaagttg ctttttttca cctgttttaa
      901 tatttaatcg atacggctca aataatgccg gattattttt attcattgca ataattacct
      961 catccggaaa atcggtaagc tcatcaatta cttcaattct gacattttgt tccggagtta
     1021 aatcacgttt attggtgcca tctctatcaa tagcatacaa tttataattt tcatcacctg
     1081 cagcatcttt gatatataat aatgtagtat tgtttttcca gaaataatag gtaatatccc
     1141 tctcagttcc ggaagttacg cgtaccggcg ctgctgtaat atcagcaggt tgcacataaa
     1201 tatttaatcg cgaattataa gggcctaaat aagcaatata atcgccatca ggagaaatag
     1261 taaaagccgt tttttcggga tttttaaaaa atgcctcgac aggaattgtg cctttgtcga
     1321 gcgcaattaa tcgttgtatt tctatagagt cgactttggc agaatgttcg ggattagctt
     1381 ttgagcagtt tgaaatactt accatagtaa ttcctccggc tattgccaat aggatagata
     1441 ccattaatcc aaacttccat tttcttctca atccaaacat attctcaaca aatttattct
     1501 gcttaacgac aaatagatgt tataaaatcc ataaaagcat gaatcgttaa cagtgtttaa
     1561 cacaaaacag cgggcacaaa aaaaggaaac ctattggtct ccttcaaata tttggtaaga
     1621 aaactggtga ttacttcgat agttccacca atttatcttc aagttcatat tcatctcctt
     1681 caatataacc ggtatgttta tcggttatga gacctgcctt gttagtcacc acagtgaatg
     1741 gaacagactg cccaccaagt atacgttgca ggtctttgtt agtatctaac aacaccgtat
     1801 aagtccaacc ctggccttca gcatatgcct tcacattagc cttagtttta gcgtcatcta
     1861 tagatacagc aatgagttca acattgtagt ctttttgcca atcttcataa atctcagcga
     1921 tattattcaa ttcctctttg caaggaacac accaggttgc ccaaaagcta aaaacctgaa
     1981 ttttatcgtc tttaccaaaa tcggctatgt ttactttatt accattaata tcataaagtg
     2041 tcaaatccgg caatgcatct tgtgcttgca gattatttaa accaaagcag gcaatagcca
     2101 ataggagacc gatttttttc attttttgat gatttagcag ttcaaagata ggttttgatt
     2161 gtcaatattt atgccatagt caataccgga taggttcgtt ttctcacagt atggttagat
     2221 tttgacaaaa aaatggcaaa aattgcggct aacatatatt gctacatgcg tttgaacttc
     2281 acccgcaaaa cttattgcac taaagccatt tttaagacct tatttgaata agaagtggct
     2341 tatcaa
//
LOCUS       DAORKW010000569         2345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2757_length_2345_cov_5.651965, whole genome shotgun sequence.
ACCESSION   DAORKW010000569 DAORKW010000000
VERSION     DAORKW010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2757_length_2345_cov_5.651965"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLB46_15605"
     CDS             <1..677
                     /locus_tag="PLB46_15605"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRF78005.1"
                     /translation="TLFKTDLKIDTAADGIIWVNRVDNWKNFGVVELNNDGVITNFVE
                     KPDHFVSDLAIIGVYYIKDGALLRSEMQYLLDNNIKEKGEYQLTNALESMKQKGYKFT
                     PGKVEEWLDCGNKDATVYTNQRVLELKKGKEQLIAGSVKTVNSVIIDPCYLGENVVIE
                     NAIIGPHVSIGSNSTVKNSLIRNSILQNSVTIADKNIANSMIGSFAVLQGKPDDLSIS
                     DYSTQA"
     gene            674..>2345
                     /locus_tag="PLB46_15610"
     CDS             674..>2345
                     /locus_tag="PLB46_15610"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF78006.1"
                     /translation="MIKTIKQFSLLTACCLMLLAAACTGSKSAQNTNPSTVSPNITAK
                     SKKGVDTAAIERDYIDACKFMALGEYTKAVELLNKIVKQDPGNDASFYQLSRIYFDFG
                     QASDALAYIKNAVNLKPENTEYLLQYADILGYSASYLKAADIYTVVVKSGKANDETYY
                     RLAYSYEKANKIDDAIKTMMSLKDIIGKEEGVVFELQRLHALNGNYESAITWMKELIE
                     MNPGNTTYMRFLSEYYEKNNQPELAAETFDILLATDTNNTDLQFKKASLFQKSGDEKL
                     YYDTMRKAFLNSAGNIDTKIFYLVLFVDSIDKKQFVMKDSVLKWTEYLVTAHADDAKS
                     FAMRGDFLFYSNALESAAANYSKSIQLRSDIFDVWIKMFYIWSDLRKYDSLVDVSATA
                     IELYPNQPVSYYFNAIGNNQLKQYNEAIKVLKRGLPLTVSNQQLRADMFTQLGDAYHE
                     LGNHIESDKAFESSIEIVADNPYTLNNYAYYLSLRNEKLERAAEMSLQSIRIVPNNPS
                     FEDTYGWILYQQKKYTEAEKWINNSLRNGGDRSGVVNEHYGDVLYQLGD"
BASE COUNT          809 a          374 c          471 g          691 t
ORIGIN      
        1 acactttatt taaaaccgat ttgaaaattg atactgctgc cgatggtata atttgggtaa
       61 accgcgtaga caactggaaa aactttggtg tggttgaatt aaataacgat ggtgtaatta
      121 ccaattttgt cgaaaaaccg gaccattttg tgtccgattt agctataatc ggggtttatt
      181 atattaagga tggggcactt ttgcgcagcg aaatgcaata tttgctcgac aataacatta
      241 aagaaaaagg cgaataccag ctaaccaatg cgttggaaag catgaaacaa aaggggtata
      301 aatttacgcc cggaaaagtg gaagaatggc tcgattgtgg taataaagat gccactgtat
      361 ataccaatca gcgcgtgctg gagttgaaaa aaggaaagga acaattaatt gcaggcagtg
      421 ttaaaactgt caatagcgta ataattgatc cttgttattt aggtgaaaat gtggtgattg
      481 aaaatgccat tattggtccg catgtttcga ttggaagtaa ttcaacagtt aaaaattcgt
      541 taatacgaaa ttctattctg caaaattcag ttactattgc agataaaaat atcgcgaatt
      601 cgatgattgg aagttttgcc gtattacaag gtaaacccga tgatttgagt atcagcgatt
      661 acagtactca ggcatgataa aaaccattaa acaattttct cttctcactg catgttgcct
      721 tatgttgttg gcagcggctt gtacaggaag taaatcggca caaaatacaa atccttcaac
      781 agtttcgccc aatattactg caaagagtaa aaaaggggta gacactgctg ctattgaacg
      841 cgattacatc gacgcatgta aatttatggc tttaggtgag tatactaaag cagtggaatt
      901 gctcaacaaa attgttaaac aagacccggg aaacgatgca tcattttatc agttgagtcg
      961 tatttatttc gattttggtc aggcttctga tgcacttgcc tatattaaaa atgctgtcaa
     1021 cctaaaacct gaaaataccg aatacctgtt acagtatgcc gatattttag gctatagtgc
     1081 cagttattta aaagcagcag atatttatac tgttgttgta aaatccggca aagccaacga
     1141 tgaaacttat tatcgtttag cctatagtta tgaaaaggca aataaaatag atgatgccat
     1201 taaaacaatg atgtcgttaa aagatattat tggaaaagaa gaaggtgtgg tttttgaatt
     1261 acaacgtctg catgcattaa acggtaatta tgaatcagcc attacctgga tgaaggagct
     1321 gatagagatg aatcccggta acactactta catgcgcttt ttgagcgaat attatgaaaa
     1381 aaataatcag ccggaactcg cagccgaaac atttgatatt ttattagcaa cagataccaa
     1441 taacaccgat ttacaattta aaaaagcaag tttatttcaa aaatcgggtg atgagaaatt
     1501 gtattacgac accatgcgca aagcgttttt aaacagtgcc ggaaatattg atactaaaat
     1561 attttacctc gtattatttg tagactctat tgataaaaaa caatttgtaa tgaaagattc
     1621 tgtgcttaaa tggacagaat atttagtgac tgctcatgcc gacgatgcaa agtcgtttgc
     1681 catgcgtggc gattttttgt tttatagcaa tgcgcttgaa tcggctgccg ctaattattc
     1741 aaaaagtata caattacgca gcgatatttt tgatgtttgg ataaaaatgt tttacatctg
     1801 gtccgattta agaaaatacg attcgttggt agatgtatca gcaacagcta ttgaattgta
     1861 tcctaatcaa ccggtaagtt attattttaa tgctattggt aataatcagt taaaacaata
     1921 caacgaagct ataaaagtat taaaacgcgg attaccctta actgtttcga accagcaatt
     1981 acgcgcagat atgtttactc agttaggaga tgcctatcac gaattaggca accatatcga
     2041 atcggacaaa gcattcgaaa gcagtattga gattgtagcg gataatccgt ataccttaaa
     2101 taattacgcg tattatttat cgttgcgaaa cgaaaagctc gaaagagcag cagaaatgtc
     2161 acttcagagc attcgcattg tgcccaacaa tccttcgttt gaagatactt atggatggat
     2221 attataccag cagaaaaaat atacggaagc agaaaaatgg attaataact ccctgcgcaa
     2281 tggtggagat agaagtgggg ttgtaaacga acattacggt gatgttttat atcagttagg
     2341 agata
//
LOCUS       DAORKW010000570         2325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2797_length_2325_cov_3.715859, whole genome shotgun sequence.
ACCESSION   DAORKW010000570 DAORKW010000000
VERSION     DAORKW010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2797_length_2325_cov_3.715859"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2231)
                     /gene="gyrA"
                     /locus_tag="PLB46_15615"
     CDS             complement(<1..2231)
                     /gene="gyrA"
                     /locus_tag="PLB46_15615"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRF78007.1"
                     /translation="MAEGEKIIPINIEEEMKTAYIDYSMSVIVSRAIPDVRDGLKPVH
                     RRVLYGMKELGVNYNKPYKKSARIVGEVLGKYHPHGDTSVYDAMVRMAQEWSMRYPLV
                     DGQGNFGSVDGDSPAAMRYTEARFSKIAEEMLGDLDKETVDFSNNFDDTLEEPTVLPS
                     KIPNLLINGSSGIAVGMATNILPHNLSEICDGIVEYIRNREITIPELMKYIKGPDFPT
                     GATIYGVEGIHESFETGRGRIVVRGVANIETSPNGREHIVITELPYQVNKALFITKIS
                     ELVHEKKIEGISDANDYSDREGMRVVIDLKKDAVATVVLNQLYKYTPLQTSYGVNNIC
                     LHQGRPKLLNLKQMIAYFVDFRHEVVVRRTQYDLRKAEERAHILEGLLIALDHLDEVI
                     ALIRGSKTPDEAKEGLITRFGLSDLQSKAILEMRLQRLTGLERDKIRDEYNEVMAHIA
                     RLREILANEDMRMQIIVDETLEIKEKYGDERRTNIVAAEGEMSIEDLIEEEDVAITIS
                     HMGYIKRTSLTEYRRQGRGGKGAKGGATRDEDFIEHIFIASTHDTMLFFSQMGKCFWL
                     PVYQIPEGTRTSKGRAIQNMISLPPDDKIMAYIDIHDLEDEAFINEHYLVFCTKKGTI
                     KKTTVEAFSRPRANGIVAINIAEGDELLAVRLTNGNCEIMMAVKSGRAIRFPEEKVRP
                     MGRTATGVIGIELADDQDEVVGMITVEKGDKEKTVLVVSENGYGKRSQLVDQETGEQE
                     YRIT"
BASE COUNT          607 a          560 c          428 g          730 t
ORIGIN      
        1 gtaatacgat attcctgttc accggtttcc tggtcaacta actgactgcg tttgccatat
       61 ccattttcac ttacaaccaa aactgttttc tctttatcgc ccttctcaac agtaatcatg
      121 ccaactactt cgtcctgatc gtcagcgagt tcgataccaa ttacccctgt tgccgtacgg
      181 cccattggac gcactttttc ttcagggaaa cggatagctc taccgctttt tactgccatc
      241 ataatttcgc aattgccatt ggttaaacga acggctaata actcatcgcc ttctgcaata
      301 ttgatagcga caataccatt ggctcttggg cgactaaacg cttctaccgt tgtttttttg
      361 atggtacctt ttttggtaca gaaaacaagg taatgctcgt ttataaatgc ctcatcctca
      421 aggtcatgga tatcgatata cgccataatc ttatcatccg gaggtaaact aatcatattt
      481 tgaatagctc ttcctttgga tgtacgggtg ccttccggaa tttgatatac cggcagccag
      541 aaacattttc ccatttgaga aaagaataac atggtatcat gtgtactggc aataaaaatg
      601 tgttcaataa agtcctcatc cctggtagca ccaccttttg cacctttacc accgcgtccc
      661 tgacgacggt attcggtaag tgatgtgcgt ttgatgtaac ccatgtgtga aatggtaatc
      721 gccacatctt cttcctcaat caggtcttcg atgctcattt caccttcagc agcaacaata
      781 ttcgttctcc gttcatcacc gtatttttct ttaatttcaa gggtttcgtc gacaataatc
      841 tgcatacgca tatcctcatt cgccaaaatt tcgcgtaagc gggcaatatg tgccattact
      901 tcgttgtatt catcgcgaat tttatcgcgt tccagtccgg ttaaacgctg taaacgcatt
      961 tcgaggatag cttttgattg caaatcgctc aatccgaaac gagttattaa cccttctttt
     1021 gcctcatccg gtgtttttga accacgaata agcgcaatta cctcatctaa atggtcgagg
     1081 gcaatgagta aaccttccaa aatatgggca cgctcctcag ccttacgtaa atcgtactgt
     1141 gttctgcgca caacaacttc atggcggaaa tccacgaaat aagcaatcat ttgcttcagg
     1201 ttaagcaatt ttggtctgcc ctgatgcaaa cagatattgt taacaccata cgaagtttgt
     1261 aatggggtgt atttatacaa ttggtttaac actacagttg ctacggcatc tttctttaag
     1321 tcgattacca cacgcatacc ttcacggtct gagtagtcat tagcgtctga aattccttca
     1381 attttcttct catgcaccaa ttctgatatt ttggtaatga aaagtgcttt attcacctga
     1441 taaggcaact cggtaattac aatgtgttca cgaccattag gcgaagtttc gatatttgcc
     1501 accccgcgaa ccactattcg gccgcgaccg gtttcaaaac tttcgtgaat gccttctacc
     1561 ccataaatgg tagctcctgt tgggaaatcc ggccctttga tatacttcat taactccgga
     1621 atggtgattt cgcgattgcg gatatactca acaataccat cacatatttc agataagttg
     1681 tgaggaagta tattagtcgc catacccacc gcaataccgg atgaaccgtt aatgagcagg
     1741 ttaggtattt tagaaggcaa tacggttggt tcttccagcg tatcgtcaaa gttgttactg
     1801 aagtctactg tttctttgtc taggtcgccc aacatctcct cggcaatctt cgaaaatctt
     1861 gcttcagtat atcgcattgc agcgggacta tcaccatcta cagaaccaaa gtttccctgt
     1921 ccgtccacta acggataacg catactccat tcctgtgcca tacgcaccat ggcatcgtaa
     1981 actgatgtat cgccgtgcgg gtgatattta ccaagcacct cccctacaat acgggcagat
     2041 tttttatagg gcttgttata gtttacgcca agttctttca taccgtacaa aacgcggcgg
     2101 tgaacgggtt ttaagccatc acgcacatcc ggaatcgcac gggaaacaat caccgacatc
     2161 gaataatcga tgtaagccgt tttcatttcc tcttcaatgt taataggaat aattttttcg
     2221 ccttcagcca tgtctttttt ctccttttac tggtagttta ttttctgctt aaaaaaatgg
     2281 tgttttttca gcgtacaaag gtagtaattt tagtccctaa accct
//
LOCUS       DAORKW010000571         2324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2799_length_2324_cov_3.875275, whole genome shotgun sequence.
ACCESSION   DAORKW010000571 DAORKW010000000
VERSION     DAORKW010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2799_length_2324_cov_3.875275"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="PLB46_15620"
     CDS             complement(<1..1701)
                     /locus_tag="PLB46_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF78008.1"
                     /translation="MKILISYLKPHKWLVFFVLLLAALNTGFSLFDPIILGRLVNLAS
                     DYSQHGDQYDARRYFSSFSWKSPGVLMLLLGSVSVAMVSRIAKNFQDYFLNVIIQKFG
                     AKVFTDGLQHAMKLPYQDFEDQRSGETLSILTKVRTDVERFMNMFINVLLGILVGVVF
                     VFVYAALFIHWYIPAVYFVGIILLTFISNILSKKVKIIQKNIVGQTTALAGATTESLR
                     NIELVKSLGLTTQEVQRLNKNTYKILGLELTKVKRIRSISFIQGTFVNTLRQIILYIL
                     MWLIFKDEMDAGQLVTMQVFSFFVFGPLQDIGNIILSYREAEASLNNFSTLMNKIPEA
                     HPEKPAALGEIQSLQFDEVKFQDKTASNPAIDGISFEVKQGETIAFVGPSGSGKSTLM
                     KLLVGLYRPQEGKILYNNIDENSIDFNDLRNQIGFVTQDTQLFAGTIKENLLFVYPEA
                     TDADLLDVLQKASCNNLLSRAEKGIETVIGEGGLKLSGGEKQRLSIARALLRRPRLLI
                     FDEATSALDSLTEEEISNTIRDISTHGVQITILIAHRLSTIMHADRIYVLEHGEIVEQ
                     G"
     gene            complement(1796..>2324)
                     /locus_tag="PLB46_15625"
     CDS             complement(1796..>2324)
                     /locus_tag="PLB46_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRF78009.1"
                     /translation="LLIAILFLVGIMNKIPYCALAVILISTGYNLAKPKMIKAVYHQG
                     KEQFFPFIVTLLAILFTDLLIGVLIGVVYAIYFLIKHTYEAGYTIKQKTEGHTSHFII
                     ELALNVSFLNKKRIQVELDKIPAYSIVEIIGTDSVYIDKDVLEIFQEYKVKAHNKHIQ
                     LIMTNIPEVKILELH"
BASE COUNT          790 a          488 c          316 g          730 t
ORIGIN      
        1 gccttgctca acaatttctc catgctcgag aacataaatt ctatctgcat gcataattgt
       61 acttaaccta tgcgcaatta aaatcgtaat ttgaactcca tgtgtagaaa tatcccttat
      121 cgtatttgaa atttcttctt ccgttaatga atcaagcgca gaagtagcct catcaaaaat
      181 caacaaacgt ggtcttctta ataaagccct cgcaatactc aatcgctgct tctctcctcc
      241 cgacaatttt aatcccccct cacctattac cgtctcaatt cccttttctg cacgacttaa
      301 caaattatta caacttgctt tttgtaacac atccaataaa tcggcatccg ttgcttcagg
      361 ataaacaaat aacaaattct ctttaattgt ccctgcaaat aattgtgtat cctgtgtaac
      421 aaacccgatt tggttgcgca agtcattaaa atcaatcgaa ttttcatcga tattattata
      481 taatattttg ccctcctgtg gcctgtacaa ccccaccaac aatttcatca acgtactttt
      541 tccactgcct gaaggcccaa caaacgcaat agtctcaccc tgttttacct caaacgaaat
      601 accatcgata gcaggattac tcgcagtttt atcctgaaat ttaacctcat caaattgaag
      661 tgattgaatt tctcctaaag cagcgggttt ttccggatgc gcttcgggaa ttttattcat
      721 taatgtacta aaattattta aagaggcttc cgcttcacga tacgataaaa tgatattacc
      781 aatatcttgc aaaggaccaa atacaaaaaa tgaaaatacc tgcatagtaa ctaactgacc
      841 ggcatccatt tcatctttaa atattaacca cattaaaata tataaaataa tctgacgcaa
      901 tgtatttaca aacgtaccct gaataaagct gatacttcgt attcttttta ctttagttaa
      961 ttccagtcct aatattttat aggtattttt atttaatcgc tgaacttcct gggtagttaa
     1021 tcctaaactt tttaccaact caatgtttct taacgattcg gtagtagcac ctgccaaagc
     1081 tgttgtttga ccaacaatat ttttctgaat aatttttact tttttactca gtatgttaga
     1141 aataaaagta agtaaaataa taccaacaaa atataccgca ggaatatacc aatgaataaa
     1201 taaagcagca tatacaaaca caaaaaccac ccccactaat atgcccaata acacattaat
     1261 aaacatgttc ataaatcgct caacatcggt tcttactttg gttaaaatgg aaagcgtttc
     1321 accacttcgc tgatcttcaa agtcctgata tggcaatttc atagcatgtt gtaaaccatc
     1381 ggtaaacacc ttcgcgccaa acttttgtat aataacattg aggaaataat cctggaaatt
     1441 tttcgcaatg cgacttacca ttgccaccga aaccgaacct aaaagtaaca tcaaaacgcc
     1501 cggacttttc caggaaaacg aactgaaata acgtctggca tcatactggt cgccatgttg
     1561 actataatcg cttgctaagt ttaccaaacg ccccaatatg attggatcga ataaagaaaa
     1621 gccggtattc aatgctgcca ataacaacac aaaaaacacc aaccacttgt gtggtttcaa
     1681 ataagatatt aatattttca taatttattg cccgaagaat atacaactca aatgtaaccc
     1741 tctgagttcg gaaaaagaaa tattagttat gtgcaaaagc caagtcttta ggggttcaat
     1801 gtaactcaag gattttcact tccggtatat tggtcatgat tagctgaata tgtttattat
     1861 gtgcctttac cttatactcc tgaaatatct ccagcacatc cttgtctata taaacactat
     1921 cggtgccaat tatttctaca atgctataag caggaatttt atccaactca acctgtatgc
     1981 gttttttatt tagaaaactc acatttaatg caagttcaat aatgaaatga gaggtatgac
     2041 cttctgtttt ttgtttaatg gtatatccgg cttcgtaggt atgtttaatt aaaaaataaa
     2101 tggcatacac cacacctatc aatacgccaa ttaataaatc ggtaaataat atagctaata
     2161 aagtgactat aaatggaaaa aattgttctt taccctgatg ataaacagct ttaatcattt
     2221 ttggttttgc caaattatat ccggtgctaa ttaaaataac ggcaagtgcg caatacggta
     2281 ttttgttcat tatcccaacc aaaaacaaaa tagcaataag caac
//
LOCUS       DAORKW010000572         2316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2808_length_2316_cov_4.619637, whole genome shotgun sequence.
ACCESSION   DAORKW010000572 DAORKW010000000
VERSION     DAORKW010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2808_length_2316_cov_4.619637"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLB46_15630"
     CDS             <1..296
                     /locus_tag="PLB46_15630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78010.1"
                     /translation="IVKLLQSVETIDEEQIQDLFISFPNPTSAILYLATSETVSNGIV
                     TICNLAGQELLHVQLDEAHQRIIPIDVHSLAAGLYFITLTANDRQQVQQFIKY"
     gene            299..>2316
                     /locus_tag="PLB46_15635"
     CDS             299..>2316
                     /locus_tag="PLB46_15635"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRF78011.1"
                     /translation="MKKYINYMMMLCLMALSCTTFAQSPKPNFIFLVVDDLNDGIEAL
                     NALPQTLTPNINRICENGTVFTNAVCPAPLCCPSRTSFLTGKDPAYTQIYSTYNYKCT
                     DFSQNFSDENNNATYFTIPGYLKDSAGYFTYGLNKIFHCYENYQEYDSLTVDACNKKL
                     SWNKIFVYNDSTILKPGINIEEQGVRNNPWSKINDTLEPYMMDYVAVDSSIQFIKHFA
                     AGEGTCGNPFFLALGIKKPHEPLYIPAKYFDSSYIEDFTASFKLPFNFPFNAYPPNGI
                     LLPPQPEIVFEDFTSLPENGMAQEMVKGADEKFELWVEGLDEVPELNTNYNTMLNEDL
                     LAWTKRANSVMAYQAGVKYVDAQIGRLLDSLSQYPAVYNNTIIILVGDNGYSLGQKKH
                     WGKRAMWETDVRVPLVIADLRNPLPNSTNVTVNLLDIFPTVCALAEINPPKFESGLPY
                     LDGKALTTIMQEPQTALELPQLSAVKKEFESEGFCYPQYAVRNERFHYIRYQSNGGDM
                     LNCDSASSYFEEELYEIGENRMVDPYEWHNLIQNAAYYPAAQYLQQWLPGNTMYLKQG
                     YKVNIVSETQTCIANKNDTLLLNINVFDLNGTLSAEIPDMQYVWTNNFTADTLIGKEV
                     HFPIALIPEKNYNNGDPLYIYFSLIANGIKITMGFDLQVFSVNPAQMP"
BASE COUNT          791 a          387 c          417 g          721 t
ORIGIN      
        1 gtatagtcaa attattacaa tcggttgaaa caatagatga agaacaaatt caggatttgt
       61 ttatcagttt tccaaatccg acatcggcta ttttgtattt agctacaagc gaaacagttt
      121 ccaatggcat tgtaacaata tgcaatttag ccggacagga attattgcat gtgcaattag
      181 atgaagcgca tcaacgtata atacctattg atgtacattc gctagctgca ggattatatt
      241 ttattacttt aaccgccaac gacagacaac aagttcaaca atttataaaa tattaatggt
      301 gaaaaagtat attaattata tgatgatgtt gtgtttaatg gcactgtcat gtactacttt
      361 tgcgcaatca ccaaaaccta atttcatttt tttagttgtt gacgatttga acgatggtat
      421 agaagcatta aatgctttgc ctcaaaccct tacacctaac atcaaccgaa tttgcgaaaa
      481 cggaactgtt tttaccaacg ccgtttgtcc ggctccatta tgttgtcctt cccgaacaag
      541 ttttttaacc gggaaagatc cggcttatac acagatttac agcacttaca attacaagtg
      601 tactgatttt agtcagaatt ttagtgatga aaataataat gccacctatt ttacaattcc
      661 tggatattta aaagacagtg caggttattt cacctatggt cttaataaaa tattccactg
      721 ttatgaaaat tatcaggagt acgacagcct gactgttgat gcctgtaata aaaaactatc
      781 gtggaataaa atttttgttt acaacgacag tacaatttta aagcccggaa taaatattga
      841 agaacaaggc gtgagaaata atccatggtc gaaaataaat gatactttgg agccatacat
      901 gatggattat gttgcagttg attcatctat tcaatttatt aaacattttg cggccggtga
      961 aggaacatgt ggtaatccat tctttttagc ccttggtatt aaaaaaccac atgaaccatt
     1021 atatattcct gcaaaatatt tcgactcatc atatattgag gattttactg cctcctttaa
     1081 attgccattt aattttccat ttaacgctta cccaccaaat ggtatattat taccaccgca
     1141 acctgaaata gtttttgagg actttacaag tttacctgaa aatggcatgg cacaagaaat
     1201 ggtaaaaggc gccgacgaaa aatttgagtt atgggtagaa ggtcttgacg aggtaccgga
     1261 attaaatacg aattataaca cgatgcttaa tgaggattta ttagcgtgga caaaaagagc
     1321 aaatagtgta atggcatatc aggccggtgt aaaatatgta gacgcacaaa ttggaagatt
     1381 attggatagt ttatctcaat atccggcagt atataataat acaattatta ttttagttgg
     1441 cgacaatggt tatagtttag ggcagaaaaa acattggggt aaacgcgcta tgtgggaaac
     1501 agatgtacgt gttccattag taatagccga tttaagaaat cctttgccaa actctaccaa
     1561 tgtaacggtt aacttgttag atatttttcc gacagtttgt gctttagcag aaattaatcc
     1621 gccaaaattt gaatcaggtt tgccttattt agatggcaaa gcgctcacca caattatgca
     1681 ggagccacaa acagcacttg aattaccaca attatctgct gtaaaaaaag aatttgaatc
     1741 cgaaggattt tgttatccgc agtatgcggt gcggaatgag cggttccatt atattcgata
     1801 tcaatccaat ggtggagata tgctaaattg tgatagcgca tcaagttatt ttgaggaaga
     1861 actgtatgaa ataggtgaaa acagaatggt tgacccttat gaatggcata atttaattca
     1921 aaatgcagct tattatccgg ctgctcaata tttacaacaa tggttgccgg gaaataccat
     1981 gtatttaaaa caaggatata aggtaaatat agtaagcgaa acacaaacat gtattgccaa
     2041 taaaaatgat acacttttat taaacatcaa tgtatttgat ttaaatggta cgctttcagc
     2101 agaaattccg gatatgcaat atgtgtggac aaataatttt actgctgaca ccctcatcgg
     2161 taaagaagta catttcccga tagcattaat tcctgaaaaa aattataaca acggcgaccc
     2221 attatatatc tatttttcat taattgctaa tggaataaaa attaccatgg gatttgacct
     2281 tcaggttttt agtgttaacc cagcacaaat gcctga
//
LOCUS       DAORKW010000573         2313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2815_length_2313_cov_4.518601, whole genome shotgun sequence.
ACCESSION   DAORKW010000573 DAORKW010000000
VERSION     DAORKW010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2815_length_2313_cov_4.518601"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLB46_15640"
     CDS             <1..206
                     /locus_tag="PLB46_15640"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRF78012.1"
                     /translation="MITYLNPNWVLSDGGELCIHYPTTVTNIAPENCKCVFFKSDELE
                     HEVLITNVPRLSITGWLKTNKIF"
     gene            complement(203..1357)
                     /locus_tag="PLB46_15645"
     CDS             complement(203..1357)
                     /locus_tag="PLB46_15645"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRF78013.1"
                     /translation="MKAIASGELTLGILGGGQLGKMLIEAASNWNIQCHVLDPDAECS
                     CAHMAHTFVQGSFKDYYTVYNFGKNLKKITIEIEHVNVEALIQLEKEGKTIYPKPAVL
                     QIIQDKGKQKSFYATNDLPTAPFTLLSNLQEVLAGIQNGTITYPFVQKSCTAGYDGKG
                     VAVIRNANDLPKLLEGACVIEQLIDFEKELAVIVARDVDGKTTCFPAVEMEFHPVANL
                     VEFLLAPAIISEQAAAKAQNIAEDCITAFGLTGILAVEMFLTKSGEILINEVAPRPHN
                     SGHHTIEANYVSQYEQLLRTIFEIPMGDTTPRDHGVMVNILGEDGYTGEALYEGLKEC
                     LAIPGVFVHLYGKKITKPFRKMGHVTIVGKDANAIKATAKLVKEQLKVIA"
     gene            1406..>2313
                     /locus_tag="PLB46_15650"
     CDS             1406..>2313
                     /locus_tag="PLB46_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78014.1"
                     /translation="MRKPFIFTLLVFSLFHTPQLFGQACLFADDFADESAWEYFYWYY
                     PVTGACTSNNQTGDLTIADGTLNYDATIDANDTRFYHELGTELNNDFWTASMAFTPIQ
                     GGASGRTGHLVLSLSEGTLNPYSDTYSICENSNTDAIMLAWTSDFAAAPDNIGFTLYA
                     NDNGVITQSELLSAPYNDTYYIQLIRVAYNYIHLDVFEDEEHEIMYGQIDCFTVPTTI
                     DGLNTVQIGNLPGGSYQRKLTATIDDLCVRNLDVEAISITGPTTICIGSENDYTINTL
                     PAADIDWELPAGIVYTGDDLTTMTVT"
BASE COUNT          674 a          540 c          387 g          712 t
ORIGIN      
        1 ccatgattac ctatctaaat cccaattggg tattaagcga tggcggcgaa ttgtgtatcc
       61 attatccaac aactgtaacc aatattgcac ctgaaaattg caaatgtgtt ttttttaaaa
      121 gcgatgaact ggaacatgaa gtactcatca caaatgtccc acgattaagt attacaggtt
      181 ggctaaaaac aaataaaata ttttaagcaa tcacttttaa ttgctccttt accaattttg
      241 ctgttgcttt aatcgcattg gcatcttttc caacaatggt aacatgaccc attttgcgaa
      301 atggtttggt aattttttta ccatataaat gcacaaaaac acccggaatg gcgaggcact
      361 cttttaatcc ttcatataag gcttcaccgg tataaccatc ttcacctaaa atattcacca
      421 tcacaccatg gtcacgcgga gtagtatcac ccattggtat ttcaaaaatg gtgcgcaata
      481 attgctcata ttgcgaaaca taatttgctt ctatggtatg atgtccgctg ttatgcggtc
      541 gcggtgctac ttcgttaatt aatatttcgc ccgatttagt taaaaacatt tctaccgcta
      601 aaattcccgt caatccaaat gctgtaatac aatcctccgc aatattttgt gctttagctg
      661 cagcctgctc cgaaattatt gccggagcca ataaaaattc taccaaattc gctaccggat
      721 gaaattccat ttcaacagca ggaaaacatg tcgtttttcc atcaacatct cttgccacaa
      781 ttacagctaa ttctttttca aaatcaatta actgctcaat cacacatgca ccttccaata
      841 atttaggcaa atcgtttgca tttcgaatta ctgcaacacc ttttccatca tatccggcag
      901 tacatgattt ttggacaaac ggataagtaa ttgttccgtt ttgaattcct gcaagcactt
      961 cttgtaaatt cgacaataag gtaaacggag cagtgggtaa atcatttgta gcataaaacg
     1021 atttttgttt ccctttgtcc tgaattattt gcaaaacagc cggtttcgga taaatcgttt
     1081 ttccttcttt ttctaattga attaatgctt caacatttac atgttctatt tcaatggtaa
     1141 tttttttcag atttttacca aaattgtaaa ccgtataata atctttaaaa ctaccctgca
     1201 caaaagtatg tgccatatgt gcacaactac attcggcatc ggggtccagc acatgacact
     1261 gaatattcca gttgctggca gcttcaatca acatttttcc taattgcccg ccacctaaaa
     1321 tacctaaagt taattcgccc gatgctatcg ctttcatgac gcaaaattaa tatccctttc
     1381 atacaaaatc actaaatttg cccacatgcg gaaacccttt atttttaccc ttttggtgtt
     1441 ttccctattt cacacaccac aactatttgg acaagcttgt ttgtttgctg acgattttgc
     1501 tgatgaatcg gcatgggaat acttttattg gtattacccg gtaacaggag cttgcacatc
     1561 gaataatcaa accggcgact taaccattgc agatggtacg ctcaactatg atgctactat
     1621 cgatgcgaat gacacccggt tttatcacga gctgggcacc gaattaaaca acgacttctg
     1681 gacagcttcc atggccttta cgccaattca gggaggcgcc tcaggaagaa cagggcatct
     1741 cgttttatcg ctttcggaag gtacccttaa cccatattcg gacacctact ccatttgcga
     1801 aaactcgaat accgatgcca ttatgctggc ctggaccagc gattttgcgg ccgctcctga
     1861 caatattggg tttacattat atgccaacga caacggtgtg ataacccaat cggaattgct
     1921 ctctgcgcct tataatgata cttattatat ccaacttatt cgtgttgcat acaactacat
     1981 tcatcttgat gtttttgaag atgaggaaca cgaaatcatg tacgggcaaa tcgactgttt
     2041 tacagtgcct acaaccatcg acggactaaa caccgtgcaa atcggcaacc ttcccggagg
     2101 ctcgtatcag cgcaaattaa cagctactat cgacgactta tgtgtccgca atctcgatgt
     2161 tgaagccatt tcaattaccg gtcctaccac catttgtata ggttccgaaa acgattatac
     2221 catcaacact ttacccgcag ctgatatcga ttgggaatta cccgcaggta ttgtttacac
     2281 cggcgacgac cttacaacca tgaccgttac cga
//
LOCUS       DAORKW010000574         2312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2818_length_2312_cov_5.449269, whole genome shotgun sequence.
ACCESSION   DAORKW010000574 DAORKW010000000
VERSION     DAORKW010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2818_length_2312_cov_5.449269"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLB46_15655"
     CDS             complement(<1..499)
                     /locus_tag="PLB46_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRF78015.1"
                     /translation="MKSYLSLITFSHTVFALPFAIIGFTLGITQPGAQFLWTDLLLVI
                     ACMVFARSAAMAFNRYIDADIDKKNARTAQREIPKGVITRKNALYFVIANAIAFMLCA
                     LMLNTLCFYLSPVALLVILGYSYTKRFTWLCHFILGIGLALAPIGAYLAVTETFQTIP
                     IIFSLI"
     gene            complement(512..2176)
                     /locus_tag="PLB46_15660"
     CDS             complement(512..2176)
                     /locus_tag="PLB46_15660"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRF78016.1"
                     /translation="MLSNKEVSAIFKTIGGLMELHGENDFKTKSYANAAFQIGRIHEH
                     VMELEEDDLAKRQGIGKSVAAKIVELRNTGTIAALEELMAITPPGIIEILRIKGLGGK
                     KVGVIWRELGIETIGELLYACNENRLSKLKGFGEKTQESVIQSINYYQSNQGKFRYAT
                     IAALVDDMLSVMQQQLAHGKIALCGAIRRQDNIIDVVQFIVNKSANISQHLSAFPFLE
                     IKEQTETLITGKAQNDIHFQIIIVEDAQFYFELFVRTGDEDFVNKVLPKLNGNTQFDA
                     EEALFEAAGEPYRAPELRVGAINKLDSGNPIVTLADIQGVVHNHSTWSDGKNTVAEMA
                     EACKMNGYTYFVISDHSKTAVYAGGLTEAQIIAQQKEISALNTSAGIKIYSSIECDIL
                     NDGTLDYDDTVLKSFDLVIVSIHQNLKMDIGKATQRLLRAIENPYTTILGHMTGRLLL
                     SRPGYPVDHKKIIDACAANDVVIEINANPYRLDIDHTYIPYAMEKGVMISVNPDAHSI
                     EGIGDIKWGVISARKGGLTKAMCWNAQSLETVDNWLIARKKKKGIL"
BASE COUNT          728 a          500 c          359 g          725 t
ORIGIN      
        1 caattaaaga aaaaataatt ggtatggttt gaaaagtttc tgtaaccgct aaataagcac
       61 caataggcgc cagagccaaa cctattccta aaataaaatg gcataaccac gtaaatcgtt
      121 ttgtatagga ataccccaaa ataaccaata aagccacagg tgataaatag aaacataacg
      181 tatttaacat caatgcacat aacataaatg ctatggcatt ggcaataaca aaatacaatg
      241 catttttacg ggttattaca cccttcggga tttcacgttg tgcagtccgg gcattttttt
      301 tatctatatc agcatcaatg tatcggttaa acgccattgc tgcgcttcgt gcgaaaacca
      361 tacaggctat caccaacaat aaatcggtcc ataaaaattg cgcaccgggt tgggtaatac
      421 ctaaggtaaa accaataatg gcaaagggta aggcgaaaac cgtatgactg aatgtaatta
      481 atgaaaggta acttttcatg tggacacaaa gttacaaaat gccttttttc tttttccgcg
      541 ctattaacca gttgtcaacg gtttccaaag actgagcatt ccagcacatc gccttggtta
      601 aaccgccttt gcgagctgaa atcacgcccc atttaatatc gcctattcct tcaatgctgt
      661 gcgcatcggg gttgacagaa atcattaccc ctttttccat ggcataggga atataggtat
      721 gatcaatatc caaacggtat ggattggcat taatttctat caccacatca tttgccgcgc
      781 aggcatcaat aattttttta tggtctacag gataaccggg acgactcaat aataatctgc
      841 ctgtcatatg tcccaaaata gtagtatacg gattttcaat cgcgcgcaat aagcgttgtg
      901 ttgccttgcc gatatccatt tttaaatttt gatgaatact gacaattact aaatcaaaag
      961 attttaaaac ggtatcatca taatcgagtg taccatcatt taaaatatca cactcaatac
     1021 ttgaataaat tttaattcct gctgatgtat ttaacgcact aatttctttt tgttgagcaa
     1081 taatttgtgc ttcagttaat cctcctgcat atactgcagt tttactgtga tcagaaatca
     1141 caaaataggt atatccattc attttgcacg cttccgccat ttctgcaacg gtgtttttac
     1201 catcactcca tgtgctgtga ttatgcacta cgccttgaat atctgctaat gtaacaattg
     1261 gattaccact atccaactta ttaatagccc caactctcaa ctccggcgct ctgtatggct
     1321 ctcctgctgc ttcaaacaac gcttcttcgg catcaaattg ggtattgccg tttaatttgg
     1381 gtaatacttt attgacaaaa tcttcatcac ccgtacgaac aaataattca aaataaaact
     1441 gcgcatcttc aacaataatg atttggaaat gaatatcatt ttgtgcttta ccggtaataa
     1501 gtgtttcggt ttgttcctta atttccaaaa atggaaatgc gctaagatgt tgtgatatgt
     1561 ttgccgattt atttacaata aactgaacca catcaataat attatcctgc cttctgatgg
     1621 ctccgcataa tgctattttt ccatgggcta attgttgctg cattaccgat aacatgtcat
     1681 caactaaagc ggcaattgta gcatatctga atttcccctg attactttga taataattta
     1741 tcgattgaat tacactctct tgtgtttttt caccaaagcc ttttaatttg gacaaacgat
     1801 tttcattgca ggcatataac aactcaccta ttgtttctat tcccaattca cgccaaatca
     1861 ccccaacttt tttaccgcca agtcctttaa tacgcaaaat ttcaataatg cccggaggcg
     1921 taatcgccat aagctcctcc aaagcagcaa tagtcccggt attgcgcaac tcaacaattt
     1981 tagctgccac cgacttccca ataccctgcc gcttcgctaa atcatcttct tccaattcca
     2041 tcacatgttc gtggatacga ccaatctgaa acgctgcatt agcataactt ttagtcttaa
     2101 aatcattttc cccatgcagt tccattaaac cgccaatggt cttaaaaatt gctgaaactt
     2161 ctttgtttga taacatacct agccaacaaa aatacactgt ttactgtgat ttgaacctaa
     2221 aatttggaaa tgtgaaaaaa gtactattac atttgcacaa atgtttaaca atattgttaa
     2281 actaatatgt caatatgccg gaaatagaca aa
//
LOCUS       DAORKW010000575         2309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2823_length_2309_cov_4.902396, whole genome shotgun sequence.
ACCESSION   DAORKW010000575 DAORKW010000000
VERSION     DAORKW010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2823_length_2309_cov_4.902396"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..>2309
                     /gene="metH"
                     /locus_tag="PLB46_15665"
     CDS             42..>2309
                     /gene="metH"
                     /locus_tag="PLB46_15665"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRF78017.1"
                     /translation="MEKILREILSRRIMLLDGAMGSMIQQYKLTETEFRGLRFKDHPQ
                     DLRGNNDLLSLTQPHIITEIHEQYLKAGADIIETNTFSATAIAQADYALEHLAYELNL
                     VSAQLAKAAANKFTQLNPQKPRFVAGALGPTNKTLSLSPDVNNPGFRTVTFDQMAEAY
                     YEEARGLVDGGVDILLIETIFDTLNAKAAIYALDKLFAEIGRKLPVMISGTITDASGR
                     TLSGQTLEAFLVAVSHLPLLSVGLNCALGAKQLKEYIQTLSSSTDLFVSAYPNAGLPN
                     AFGEYDETPESNGNDVEVYLKNGWVNIIGGCCGTTPDHIEYIAKLAAKYPPRKPVTLP
                     LLPAFSGLEKFSLFEGSNFVNVGERTNITGSKQFKRLITEEKYDEALIVARQQVENGA
                     QIIDVNMDEGMIDSVNAMTTFLNLIAAEPDIAKVPIMIDSSKWEVIEAGLKCVQGKSI
                     VNSISLKEGEAVFKAHALKAKQLGAAVIVMAFDEEGQATTYEKRIEICERAYNILVNE
                     IHFTPTDIIFDPNILTIGTGIEEHNNYAVDFIQATKWIKANLPGALVSGGVSNISFAF
                     QGNNTVREAMHAAFLYHAIKAGMDMGIVNAGMIDIYENIDKDLLIKIEDVIFNRNENA
                     TENLITFAETLKKKDKAAVVTDEWRNYSVEERLSYSLVKGITEFIDSDIEEARLKYAL
                     PLDVIEGPLMAGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAYLQPFIELEKSQTGNT
                     EGRKKVLLATVKGDVH"
BASE COUNT          793 a          370 c          488 g          658 t
ORIGIN      
        1 aagtacctgt ttggtaaaat catcttgtgg taaatttgca cgtggaaaaa attttaagag
       61 aaatattgag taggcgtatc atgttgttag atggcgccat gggcagtatg attcagcagt
      121 ataaactcac agaaactgag ttcagaggtc tgcgattcaa ggaccatcca caagatttac
      181 ggggtaataa tgacttatta tcacttactc aaccacacat cataactgaa atacacgagc
      241 aatacctgaa agcaggtgca gatatcatag agaccaatac atttagcgct acagctatag
      301 ctcaggctga ttatgccctt gaacacttag cttatgagtt gaatctggta tcagcgcaac
      361 ttgctaaggc tgctgctaac aagtttactc agttaaatcc acaaaaacca cgatttgttg
      421 caggtgctct tggaccaacc aataaaacat taagcctatc gcctgatgta aataatccgg
      481 gttttcgaac tgttaccttc gaccaaatgg ctgaagccta ttatgaagaa gcgcgtggat
      541 tagtagatgg aggtgtagat atcttattaa ttgaaaccat ttttgatacc ttaaatgcaa
      601 aggcagcgat ttatgcttta gataaattat ttgctgaaat cggcagaaag ttgccggtta
      661 tgatttccgg cactataacc gatgccagtg gaagaacatt gagcgggcaa acattagaag
      721 catttttagt tgcggtgagt catcttccgt tacttagtgt aggattaaat tgcgcacttg
      781 gtgcaaaaca attaaaagaa tatatccaaa ccctgtcatc tagtaccgat ttatttgttt
      841 ctgcatatcc aaatgcaggt ttaccaaatg catttggtga atatgatgaa accccggaaa
      901 gcaatggtaa tgatgttgaa gtatatttaa aaaatggttg ggtaaatatt attggcggct
      961 gttgtggaac tacacctgac catattgaat acatcgcaaa attagctgca aaatatccac
     1021 ctcgtaaacc agttacatta ccattattac ctgcattttc aggattagaa aaattttccc
     1081 tttttgaagg cagtaatttt gtaaatgttg gtgaaagaac aaacataaca ggcagcaaac
     1141 aatttaaacg tttaattaca gaagaaaaat atgatgaagc attaattgta gcccgccagc
     1201 aagttgaaaa tggtgctcaa ataattgatg tgaacatgga tgaaggcatg attgattcag
     1261 tgaatgccat gactactttt ctgaatctga ttgctgctga acccgatatt gccaaagtgc
     1321 caataatgat tgattccagt aaatgggaag ttattgaagc gggattaaaa tgtgtgcaag
     1381 gaaaaagtat tgtaaattca atttctttaa aagaagggga agcggttttt aaagctcacg
     1441 cgctaaaagc aaaacaatta ggtgcagctg ttattgtgat ggcatttgat gaagaaggcc
     1501 aggcgaccac ctatgaaaaa agaattgaaa tttgcgaaag agcctataat attttagtta
     1561 atgaaattca ttttacgcca acagatatca ttttcgaccc taatatttta actattggaa
     1621 caggaattga agaacataat aattatgccg ttgattttat tcaggctacg aaatggatta
     1681 aagcaaattt accgggtgct ttagtgagtg gtggtgtaag taacatatca tttgcatttc
     1741 aaggtaataa tactgttcgg gaagcaatgc atgctgcatt tttatatcat gctattaaag
     1801 cgggtatgga tatgggtatt gtaaatgcag gtatgattga catctatgaa aacatcgata
     1861 aggatttatt gataaaaata gaagatgtca tttttaatcg caacgaaaat gccacagaaa
     1921 atctaatcac ttttgctgaa acactcaaga aaaaagataa agcagcagtg gttacagatg
     1981 aatggcgaaa ttatagtgtt gaagaaagat taagttattc gttggtaaaa ggcataaccg
     2041 aatttataga cagcgatata gaagaagcgc gattaaaata cgcgctgcca ctggatgtaa
     2101 ttgaagggcc gcttatggca ggcatgaatg tagttggcga tttattcggc agcgggaaaa
     2161 tgttcttacc tcaggtagta aaaagcgcac gtgtaatgaa aaaagcggtg gcttatttac
     2221 aaccttttat tgaactggaa aaatcgcaaa ccggcaacac cgaaggcagg aaaaaagtat
     2281 tactcgcaac tgtaaaaggc gatgtacat
//
LOCUS       DAORKW010000576         2304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2835_length_2304_cov_6.341485, whole genome shotgun sequence.
ACCESSION   DAORKW010000576 DAORKW010000000
VERSION     DAORKW010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2835_length_2304_cov_6.341485"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLB46_15670"
     CDS             <1..686
                     /locus_tag="PLB46_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRF78018.1"
                     /translation="EYTQKNDVLVTLGIKPTRPDTGYGYIQFEEGTVHRDIHKVKTFT
                     EKPDNELARTFIKSGDFLWNSGIFIWNVKSIMSAFDHFQQDIHQAFWAARKVFNTKDE
                     KRMLEKAYSICPSISIDYAIMERADNVFVIPAAFGWSDLGTWASLWDNYNKDYYGNAV
                     KGNNVMIYDAANNMIMTPDNKLVVIQGLDDYIVVDSEDVLLICKKDKEQEIKDITADI
                     KRKELDEFL"
     gene            813..1955
                     /locus_tag="PLB46_15675"
     CDS             813..1955
                     /locus_tag="PLB46_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HRF78019.1"
                     /translation="MQIKSKLPNSGISIFAKMTALANQHGAINLAQGFPDFPSSVRLI
                     ENVYAAMRRGFNQYAPMPGVLSLRERIAEKVFTIYGSNYSPDTEITITAGATQAIYTA
                     ITAIIREGEEVIIFEPAYDSYAPAIRLSGGVPVPVALHAPDYSIDWEMVKKNVSQRTR
                     MILINTPHNPTGAVLSKQDIVQLEKIVEGKDIFILSDEVYEHIIFDNKRHESILYYPN
                     LREKSIIVYSFGKTYHNTGWKIGYVLAPEFITKEFRSVHQFEVFSVNTPIQHALAEFM
                     QDPNEYLHVHTFYENKRNLFNNLLKKSRFKIKPASGTYFQLADYSEISNLPDMAFAEE
                     LCIQHKVAAIPLSPFYRQGSNEKILRFCFAKQDETLQKAAEILCRI"
     gene            1943..>2304
                     /locus_tag="PLB46_15680"
     CDS             1943..>2304
                     /locus_tag="PLB46_15680"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRF78020.1"
                     /translation="MQDLNVTLVQANLLWENTSGNLSHFEKLFEKITTTDLIILPEMF
                     TTGFTMQSKSFADFMEGKSVNWMRTQAEKKQAVITGSLIIEDDGKYFNRLVWMQPDGE
                     LNYYDKRHLFSLAGEEKH"
BASE COUNT          787 a          351 c          449 g          717 t
ORIGIN      
        1 tggaatacac ccaaaaaaat gatgtattag ttacacttgg aattaaacca actcgtcccg
       61 ataccggtta tggttatata caatttgagg agggcactgt tcaccgcgac attcataagg
      121 taaaaacgtt taccgaaaaa cccgataacg agttagccag aacatttata aaaagtggcg
      181 actttttatg gaattcggga atttttatct ggaatgtaaa aagtattatg tcggcctttg
      241 atcattttca gcaagacatt caccaggctt tttgggctgc acgaaaagtg tttaatacca
      301 aggatgaaaa acgtatgctc gaaaaagcat atagtatttg tccaagtata tcgattgatt
      361 atgccataat ggaaagggct gataatgttt ttgttattcc tgctgctttt ggctggagtg
      421 acttgggaac atgggcaagt ttatgggata attataataa agattattat ggcaacgcgg
      481 taaaaggcaa taatgtgatg atttatgatg cggctaataa tatgataatg acacctgata
      541 ataaacttgt ggtgatacag ggtttagatg attatattgt agtagatagt gaagatgttt
      601 tactcatttg caaaaaagat aaagaacagg aaataaaaga tataactgct gatataaagc
      661 ggaaagagct ggatgagttt ttataacaat ctggatttta atttttgagt tgacagatgt
      721 gatgcatcta caataatatt tttaatttat actaatatta tggttcctgt aaatatcgtt
      781 tacaggaatg aagtaaataa gttattcccg gcatgcaaat aaaatcgaaa ctaccgaatt
      841 caggcatcag tatttttgca aaaatgaccg cgctggccaa tcagcatggc gccataaatt
      901 tagcacaggg atttcccgat tttccttctt ctgttcgttt aattgaaaat gtttatgccg
      961 ctatgcgcag aggattcaat caatatgctc caatgccagg tgttttaagt ttaagagaac
     1021 gtattgcaga aaaagttttt actatttacg gaagtaatta ttcgccggac acggaaatta
     1081 ctattacagc cggagcaact caagcaattt atacagctat taccgctatt ataagagaag
     1141 gtgaagaagt tattattttt gaacctgcgt atgatagtta tgcacccgca atacgactga
     1201 gcggcggcgt tccggtgcct gtagcattac acgctccgga ttatagtata gattgggaga
     1261 tggtgaaaaa aaatgtttca cagcgcacgc ggatgatttt aattaatact cctcataatc
     1321 ccaccggagc agtattaagt aaacaagata ttgttcaact ggaaaaaatt gttgaaggca
     1381 aggatatttt tattttgagt gatgaagtgt atgagcatat tatttttgat aataagcgac
     1441 atgaaagtat tttatattat cccaacttaa gagaaaaaag tatcattgta tattcatttg
     1501 gtaaaacata tcacaatacc ggatggaaaa ttggttatgt acttgcgccg gaatttatta
     1561 ccaaagaatt cagaagtgta catcagtttg aagtattttc tgtcaataca ccaattcaac
     1621 atgctttggc tgaatttatg caagacccta atgaatatct tcatgtacat actttttacg
     1681 agaataaaag aaatttattt aataatttat taaaaaaatc gcgctttaaa attaaacctg
     1741 cgtccggcac atattttcaa ttagcagact attctgaaat aagtaatttg cccgacatgg
     1801 cttttgcgga agaactttgt atacaacaca aggttgctgc tattccatta tcaccttttt
     1861 accgacaagg ttctaatgaa aaaatattgc gattttgttt tgccaaacaa gatgaaactt
     1921 tacaaaaagc cgctgaaata ttatgcagga tttaaatgtg acattagtac aggcaaattt
     1981 attgtgggaa aatacatccg gcaatttatc gcattttgaa aagttatttg aaaaaataac
     2041 gactaccgat ttaattattt tacctgaaat gtttactaca ggatttacta tgcaatcgaa
     2101 aagttttgcc gatttcatgg aaggtaaaag cgtaaactgg atgcgcactc aggccgaaaa
     2161 aaaacaagcc gtaattacag gtagtttgat aattgaggat gatgggaaat attttaaccg
     2221 attagtctgg atgcagccag atggcgaact taattattac gataagcgcc atttgttttc
     2281 attagccggt gaagaaaagc acta
//
LOCUS       DAORKW010000577         2302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2838_length_2302_cov_4.084557, whole genome shotgun sequence.
ACCESSION   DAORKW010000577 DAORKW010000000
VERSION     DAORKW010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2838_length_2302_cov_4.084557"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..434)
                     /locus_tag="PLB46_15685"
     CDS             complement(90..434)
                     /locus_tag="PLB46_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78021.1"
                     /translation="MKKLFLPLLLLATSNLFAQAPAITWQNTIGGSDWDDIRTVKQTS
                     DGGYIMGGDSESGISGDKSEANQGLYDYWIVKLDSLGAIMWENTIGGSENDYCNVVIE
                     TADGGFLAAVTS"
     gene            complement(730..>2302)
                     /locus_tag="PLB46_15690"
     CDS             complement(730..>2302)
                     /locus_tag="PLB46_15690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78022.1"
                     /translation="VVVGAYNYAVGQSGEGHVFVYHGSATGLITTPARTYQNNQAGSN
                     LGRSVAMVNNLNGDAYADIVVGADGHDVDVENIGGVFVYLGSASGLSFVANDQLKGYL
                     SGANFGSKITAAGDVNLDGFEDILVGAYDDFIGEYFEGIAYLYAGNSGGMDHTPLVSF
                     QSNQASAGGSKSLSGSGDFNDDGAIDIIFGAPYYDNTESLEGAAFIYFGCTDTYFLDA
                     DGDGFGGAISITTCIPSPEYVITNSDCNDDNETIYPFASELCNGLDDNCNVNIDEGLD
                     PLITISAGGPTTFCQGSNVMLTATYTGYALQWEKNGALIPGATFPTYFASTKGNFSCV
                     VDNACGSLESSPIFVNVLKNPSATISAGGPTTFCVGGSVVLNANSGAGLSYQWFKNAV
                     EIPGATSITYTATTAGIYKCKVIKTATGCFKMSNGITVTVPCKEGEMLNGILTVYPNP
                     ATNAVNISYEGALYNTIDLQITNSIGQIVFNNNIHNYQMGDVINVDVSTFPAGIYHVR
                     FIESGRVTEQQFVVQ"
BASE COUNT          719 a          492 c          406 g          685 t
ORIGIN      
        1 gcaatattac aagatgccac tagttttacc agcgaactgt cgcgtgatat ggtgatgtta
       61 ttttcagttt tatcgccaga gatatcagat taagatgtga ctgcagcaag gaaacctccg
      121 tctgcagttt caataactac attacaataa tcattttcgc ttccgccaat ggtattttcc
      181 cacataatag cgccgaggct atctagtttt actatccaat aatcataaag accttgatta
      241 gcttcacttt tatcgccgga gataccggat tcagaatcac cgcccataat gtaaccgcca
      301 tcggatgttt gttttacggt gcggatatca tcccaatcag atccgccgat cgtgttttgc
      361 caggtaatgg caggggcttg ggcaaatagg ttagatgttg ccaatagcaa caggggtaaa
      421 aataattttt tcataaaggt gtgtgtgttt tgtgtgtgtt taaaaaggca agcaagggat
      481 aatgttctta cttacaatta cttgcgaaaa ataagtagct gcccgtaaat taaaaaccac
      541 aattgtgtga aatacaggaa attaagtgcg aaataacatt ttgagttagc gaagcaatgc
      601 aaccaggtaa taactgtgtg ggtacccttt aggtaaatat ctaaatacac ccattttatt
      661 ctgtaataat acaaatacta caaacatgta taaaccgaaa atgaggctct gccgttttcg
      721 tgtatttagt tattgtacta caaattgctg ttcagtaact ctaccactct ctataaatcg
      781 cacatgataa atacctgccg gaaatgtgct tacatcaaca tttataacat cgcccatttg
      841 gtaattgtgt atgttgttgt tgaatacaat ttgccctatt gagttggtga tttgtaaatc
      901 aattgtatta tacaaagcac cttcataact aatattcaca gcattcgtcg caggattcgg
      961 gtaaacggtt aatattccat tcaacatctc tccttcttta caaggaactg ttactgttat
     1021 tccattggac attttgaaac atccggtagc tgttttaatg accttacatt tataaatacc
     1081 ggctgttgtt gcggtgtagg taatgctggt tgccccgggt atttcaacag catttttaaa
     1141 ccactgataa cttaatccgg caccagaatt ggcatttaaa acaacacttc cgccaacaca
     1201 aaatgtggta ggaccgcctg ctgagatagt tgcagatggg ttctttaata catttacaaa
     1261 aattggagaa ctttcgaggc taccacatgc attatctact acacagctaa aattaccttt
     1321 tgtacttgca aaatacgttg gaaatgtggc acccggaatt aaagctccat ttttttccca
     1381 ctgcagtgca taaccagtat atgtagctgt tagcataaca ttacttcctt gacaaaatgt
     1441 ggttgggcca cccgcgctaa tggtaattaa gggatccaat ccttcatcga tgttcacatt
     1501 gcaattatca tcgagcccat tacatagttc gcttgcaaat ggataaatcg tttcgttatc
     1561 atcattgcaa tcactatttg taattacata ttcagggctt ggtatacatg tagtaatgga
     1621 aattgcaccg ccgaagccat caccatcagc atctaaaaaa taagtgtctg tgcatccaaa
     1681 ataaataaag gctgcgcctt ctaatgattc ggtattatcg taatatggtg caccaaaaat
     1741 aatgtcaata gcaccatcgt cattaaaatc cccagatcct gataatgatt tactgcctcc
     1801 agctgatgcc tggttagact ggaacgaaac gagtggtgta tgatccattc cgcccgaatt
     1861 tccggcatac aggtaagcaa taccctcaaa atattcacct ataaaatcgt cgtatgcacc
     1921 tacaagtata tcttcaaaac catctaaatt aacatcacca gcagcagtaa ttttcgatcc
     1981 aaaatttgca ccggacaaat atcctttcaa ctggtcattt gcaacaaaag acaaacctga
     2041 agctgaacct aaataaacaa acaccccgcc aatattttca acatcaacat cgtgtccatc
     2101 tgctcctaca acaatatcgg cgtaagcatc accatttaaa ttattaacca tggcaacaga
     2161 acgacccaaa tttgaccctg cttgattgtt ttgatacgtt ctagcaggtg ttgttatgag
     2221 accggttgcg gagccatggt atacaaatac atggccttca ccactttgcc caacagcata
     2281 gttataagcc ccaacaacaa ca
//
LOCUS       DAORKW010000578         2292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2855_length_2292_cov_4.415288, whole genome shotgun sequence.
ACCESSION   DAORKW010000578 DAORKW010000000
VERSION     DAORKW010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2855_length_2292_cov_4.415288"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLB46_15695"
     CDS             complement(<1..762)
                     /locus_tag="PLB46_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRF78023.1"
                     /translation="MLELFHQSSFICSRKVTELYSTSFTLGIRALDNRFHDAIYGIYG
                     FVRYADEIVDTFHDWDKSALLAKFKADTYEAIDQKISLNPILHAFQLTVHKYQIDNKL
                     IDAFLHSMEMDLFTQNYKQEFYEEYIYGSAEVVGLMCLKVFCEGDNAKFESLKQGARY
                     LGAAFQKVNFLRDMQADYKERGRVYFPNVDFDNFCRNQKDAIEADIQSDFEKAYEGIK
                     MLPEGAKFGVYLAYKYYLNLFKKIRKTEAEKLLHTR"
     gene            complement(825..>2292)
                     /gene="crtI"
                     /locus_tag="PLB46_15700"
     CDS             complement(825..>2292)
                     /gene="crtI"
                     /locus_tag="PLB46_15700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRF78024.1"
                     /translation="HVVVIGSGFSGISAACALAKKGYRVTVVEKNECAGGRASFFKAD
                     GFMFDMGPSWYWMPDVFEWFFNEYGSSVEEHYDLLRLDPAYRIYFAKNEITDIPASLD
                     ALYDLFEQLEPGSSIQLKKFLKDAAYKYEVGMKDLVYKPCNSIMEFADFRMVKALFTM
                     QLFTSLRKEVYGKFKNDKIRKILEFPVYFLGALPSNTPALYSILNYADLALGTWYPQG
                     GMYKIIEGMQRVAEKMGVEFLFNSPVTEIATNGNITNGVYINGDFFKADAVVAAADYN
                     HVEEKLLNTKARNYDAKYWDKKVFAPSSIIYYLGIKGKVNNLLHHNLFFDQSFEQFAD
                     EIYTHPRWPSNPLFYVSAPSKTDPSVAPDGDENLFILVPVAAGLEDTPELREKYFDMV
                     MKRIEDFTGESIKNRIVYHRSFAPKDFVSRYNAYKGNAYGLANTLLQTAIFKPKMRNK
                     HIKNLVYAGQLTVPGPGVPPSLISGYVAAGEVEKIIGH"
BASE COUNT          702 a          447 c          420 g          723 t
ORIGIN      
        1 tctggtatgc agcaattttt ctgcttctgt tttacgtatt tttttaaaga gattcagata
       61 gtatttataa gcaaggtaaa ctccgaattt tgctccttcg ggtaacattt taatgccttc
      121 gtatgccttt tcaaaatcgc tttggatgtc ggcttcaata gcatcttttt gattgcgaca
      181 aaagttatca aaatctacgt taggaaaata aacgcgaccg cgctctttgt aatctgcttg
      241 catgtcgcgt aaaaaattta ctttttgaaa ggctgcacct aaatatctgg caccttgttt
      301 cagcgattcg aattttgcat tatcgccttc gcaaaatact tttaaacaca taaggccaac
      361 tacttcggcg ctaccatata tatattcttc gtaaaattct tgcttataat tctgtgtaaa
      421 taaatccatt tccatgctat gcaaaaaagc atcgatgagt ttattgtcaa tttgatattt
      481 atgcacggtt aattgaaaag catgcaaaat tggatttaag gatatttttt ggtcaattgc
      541 ctcataagta tcagctttaa atttagccag cagagctgat ttgtcccagt cgtgaaaggt
      601 atctacaatc tcatccgcat agcgaacaaa tccataaatg ccatatatag catcatgaaa
      661 acgattgtca agagcacgaa tacccagggt gaaagaagtg ctgtaaagct cggttacctt
      721 acggctgcaa ataaaggaag attggtgaaa caactcaagc ataggtcaaa ttgagatata
      781 ttaaccctat ttacgaggat ttgttcacca gagtttaaag ttttttaatg accgataatt
      841 ttttctacct cgccggcggc tacataacct gagataagtg agggcggaac ccctggcccg
      901 ggcacagtca actgacctgc atacaccagg tttttgatat gtttattgcg cattttgggc
      961 ttaaaaatgg cagtttgaag gagtgtattc gccaggccat aagcattgcc tttataagca
     1021 ttataccgcg aaacaaagtc ttttggagcg aatgagcgat ggtaaacaat tcggttttta
     1081 atcgattcgc cggtgaaatc ttctattcgt ttcatcacca tatcaaaata tttttcgcga
     1141 agctcaggtg tatcttcgag ccctgcggca acaggaacca aaataaacag gttttcatcg
     1201 ccatcaggag caacactagg gtcggtttta gaaggcgctg aaacataaaa caaagggttc
     1261 gatggccatc gaggatgggt ataaatttcg tcggcaaatt gctcgaaact ttggtcgaaa
     1321 aacaggttgt gatgtaaaag attattcact ttacctttta tgcctaaata atagataata
     1381 ctggatggag cgaaaacttt tttatcccaa tatttagcgt cataatttct ggctttggta
     1441 ttcaataatt tttcttcaac atgattataa tctgctgcag caacaactgc atcggcttta
     1501 aagaaatcgc cattgatata aaccccattc gtaatattgc cgtttgtagc aatttcggta
     1561 acaggtgaat taaataaaaa ttcgacaccc attttttcgg ccacacgttg cattccttct
     1621 ataattttat acatgccacc ttgcggatac catgtgccta atgctaaatc ggcataattt
     1681 aaaatggagt acaaagcagg tgtattggat ggtaatgcgc ctaaaaaata aaccggaaat
     1741 tcgagtattt ttctgatttt atcattttta aacttgccat aaacttcttt ccgaagggag
     1801 gtaaacaact gcatggtaaa tagggctttc accattctga agtccgcgaa ttccataata
     1861 gaattgcagg gtttatatac aaggtctttc attccaacct cgtatttata tgcagcatct
     1921 ttaaggaact tctttaattg aattgaagac cctggttcta gttgttcgaa taaatcatac
     1981 aatgcatcta atgaagcagg aatatctgta atttcatttt tagcgaaata aattcgatat
     2041 gcaggatcta gtctaagtaa gtcgtaatgt tcttctacac ttgagccgta ttcattaaaa
     2101 aaccactcaa aaacgtcggg catccaatac cagcttggtc ccatatcaaa cataaatcca
     2161 tcggctttaa aaaaagaggc tctaccgcct gcgcattcgt ttttttctac aacagtaaca
     2221 cgatatcctt tttttgcgag ggcacatgct gctgaaattc cgctaaatcc cgaacctatt
     2281 actactacgt gt
//
LOCUS       DAORKW010000579         2276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2886_length_2276_cov_5.488969, whole genome shotgun sequence.
ACCESSION   DAORKW010000579 DAORKW010000000
VERSION     DAORKW010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2886_length_2276_cov_5.488969"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PLB46_15705"
     CDS             complement(<1..1216)
                     /locus_tag="PLB46_15705"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRF78025.1"
                     /translation="MEELSSAYGWLSIVPPLVVIVVSILLRSSFEGLLIGCVAGFILI
                     DGWGFFDGFISSLYTVMSSEDTIWVIVVCGLYGSVMGLMVRSGGALKFGERMMQYMKS
                     KKSALFGTWLLGILVFIDDYLSALTAGITMRNVTDKFKIPREYLAYIVNTLSPPLCVI
                     VPISTWTLFIGKQLETSGFAAEGQGLASYFSVIPFVAYGWVAILMIPLFIFGILPHFK
                     KMKLAIHRAETTGQTIPDGTKLSMVQFDAFPKLVNPKARYFLVPLLVVLISTVIFQDT
                     STPGIEIDALKGVLIGVAFTFFYFLISKIASVQLLTETIFTGFNSMLFALALVVVSLM
                     LKEVGDKMGLTEYVINSVTPYVNKAFLPVIIFIALSLISFTTGSSWGLYVVAIPIVIP
                     LAQHIDSNVYLNI"
     gene            complement(1240..>2276)
                     /locus_tag="PLB46_15710"
     CDS             complement(1240..>2276)
                     /locus_tag="PLB46_15710"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF78026.1"
                     /translation="RMQVIYQPMGVIGVITAWNFPAYNPARAWAAALAAGCTLVVKVS
                     EFTPLTGMNLIAALVEAGLPAGVINLVHGDAAKIGAAMLNHSAVKKISFTGSTRVGKI
                     LMDGASKTNTKLALELGGNAPVIILEDVDIEHVAKTAVATKFRNCGQVCISPQRFYVH
                     EKIFHEFSALVANYAAALIVGNGLDSETRLGPLINKTQQQHVLQIIEQAKEEKNNIIT
                     GGTAQHPGNFILPTVIEVLNKQSEFIHKEIFGPVMPLISFSTIEEVITLANQTPYGLS
                     AYIWTNNINHAIYLSESLEFGMVGINEWAPHATEAPFGGWKQSGIGHESGSEGMLEYM
                     EKKLIAIGGL"
BASE COUNT          766 a          475 c          350 g          685 t
ORIGIN      
        1 caatattgag gtaaacatta gaatcaatat gttgtgccag cggaataaca attggaattg
       61 ccaccacata taaaccccag ctagaacctg ttgtaaacga aattagtgat aaggcaataa
      121 aaataattac aggcaaaaat gccttgttta catatggtgt tacgctgttg atgacatatt
      181 cggttaaacc cattttatcg ccaacttctt ttaacatcag cgaaacaacc acaagtgcca
      241 aagcaaacaa catggaatta aatcctgtaa atattgtttc agtcaataat tgcacactgg
      301 caattttgct gattaaaaaa taaaaaaatg taaaagctac tccaattaac acacctttta
      361 gtgcatctat ttctattcct ggtgtagatg tatcctgaaa tatgacagta ctaatcaaca
      421 caactaatag tggtactaaa aaatatcttg cttttgggtt taccaattta ggaaatgcat
      481 caaattgcac cattgatagt ttggtgccat caggtattgt ttgcccggtt gtttcagcac
      541 gatgtatggc aagtttcatt tttttgaaat gcggcaaaat gccaaaaatg aacaatggaa
      601 tcatgagtat agcaacccac ccgtaagcaa caaaagggat aacagaaaaa taactggcta
      661 aaccctgccc ttctgcggca aatcctgaag tttccaattg tttgccaata aataatgtcc
      721 aggttgagat tggcacaata acacataatg gtggagaaag tgtattaaca atataggcaa
      781 gatattcacg tggaatttta aatttatcgg taacatttcg catggtaata cctgctgtca
      841 atgcgcttaa ataatcatca ataaaaacta aaataccgag taaccaagta ccaaataatg
      901 ccgatttttt agatttcata tattgcatca tacgttcccc aaatttgagg gctccaccac
      961 tgcgtaccat taatcccata actgagccat ataatccgca cacaacaatt acccaaatgg
     1021 tatcttcact tgacataact gtgtacaatg aactgataaa tccatcaaaa aaaccccatc
     1081 cgtcaattaa aataaatcca gctacacatc caatcaacaa gccttcaaat gatgacctca
     1141 ataaaattga aacaacaatt actaccaatg gtggaactat tgataaccat ccgtaagctg
     1201 agctgagttc ttccatgtaa ttaattttaa ttcgctttat tataagccac caatggcaat
     1261 taattttttc tccatatact ccaacattcc ttcgcttcca ctttcatgac caataccact
     1321 ttgtttccac ccgccaaatg gcgcttcagt agcatgaggt gcccattcat taatgccaac
     1381 catcccaaat tcaagtgatt cactcaaata aatagcatga ttgatattgt tggtccaaat
     1441 gtatgcagat aagccatatg gtgtttgatt tgctaatgtg attacttctt caatagttga
     1501 aaatgaaata agcggcataa caggtccaaa tatttcttta tgaataaatt cagattgttt
     1561 attcaaaact tcaataacag ttggcaaaat aaaattacca ggatgttggg ctgtaccacc
     1621 tgttataata ttattttttt cttcctttgc ctgctcaata atttgtaaca catgttgttg
     1681 ctgtgtttta ttaattaaag gacctaaacg tgtttcacta tctaacccgt taccaacaat
     1741 taatgctgct gcataattag ctaccaaagc ggaaaattca tgaaatattt tttcatgcac
     1801 ataaaatcgt tgaggcgaaa tacatacctg tccacaattc ctgaatttag ttgcaacagc
     1861 tgttttagcc acgtgttcaa tatccacatc ttcaagaata ataaccggcg cattgcctcc
     1921 taattccaat gcaagttttg tatttgtttt actggcacca tccatcaaaa ttttaccaac
     1981 tcttgtgcta ccggtgaaac taattttttt aaccgcacta tgatttaaca tagcagcgcc
     2041 aatttttgca gcatcgccat gcactaaatt aattacacca gccggtaatc cggcttctac
     2101 taatgcagca attaaattca taccggttaa gggcgtaaat tcacttactt taactaccaa
     2161 tgtacatcct gcagctaatg cagctgccca ggcacgtgcc gggttgtagg ccgggaaatt
     2221 ccatgcagta ataacaccga taacacccat aggctgataa attacttgca ttcgtt
//
LOCUS       DAORKW010000580         2272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2898_length_2272_cov_3.493911, whole genome shotgun sequence.
ACCESSION   DAORKW010000580 DAORKW010000000
VERSION     DAORKW010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2898_length_2272_cov_3.493911"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLB46_15715"
     CDS             complement(<1..949)
                     /locus_tag="PLB46_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78027.1"
                     /translation="MSDTLIEGFKEDGLRSIADKIHQRLSELPAEDKFSVGRWAWELL
                     QNAKDSSLDGTTVNIRMALDSNRLIFSHTGNAFTELDIRGLINVITSKEAIDGMPRRK
                     TGKFGTGFLTTHLLSTIVDVEILLLENNGSYSSTYFTINRSATSTKDLIPDIENTALQ
                     AIKNRKAVTSPAHGPQIFNTTFTYRLDAQIQREKASNAIKEFIRLLPYVLAFNPTIGS
                     IEIDAPEIGISSYKLSCKHDSNKDVYVIECESQLSIQSNRYLKVITDENKHIQIAFEI
                     DYKDSVGHILPFENTPKLFCDFPLLGSESFQLPVVINSPL"
     gene            complement(1283..1948)
                     /locus_tag="PLB46_15720"
     CDS             complement(1283..1948)
                     /locus_tag="PLB46_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78028.1"
                     /translation="MIPTHGIYCYTKAFPTTCPSCNQPVFFFFCNCGSKVYFDSLGSP
                     WPIHFCRKQAIVEAIELFRGSEYLTDIEIRRRIMDYAKQQDFIIDDLAMETIEEELGK
                     HNNKLTIYDAKLLKEEEDISGSVVNINKNINIHKVFGVQKGSILEKALLGELFGKKII
                     EFKIREKPDKKNQCKQYVVYVDEVYLKAKPIKINEFILGVARKFSNGLESIYIVKEHL
                     IVK"
     gene            complement(1952..2173)
                     /locus_tag="PLB46_15725"
     CDS             complement(1952..2173)
                     /locus_tag="PLB46_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78029.1"
                     /translation="MANHYVNFKIPKSELGKADAVFSISDDDGKIGTLKISHGAIEWK
                     PANNQYSRRLSWKQFNTLMQKEGTRVLTK"
BASE COUNT          668 a          394 c          373 g          837 t
ORIGIN      
        1 acaaaggtga attaataacc acgggtaatt gaaatgactc gcttcctaaa agcggaaaat
       61 cacaaaatag tttaggtgtg ttttcgaatg gtaaaatatg tcccactgaa tctttataat
      121 caatttcgaa tgctatttgg atgtgtttat tttcatcggt tattacttta agatatcgat
      181 tgctttgaat gcttaattgt gattcgcact caattacata gacatcttta ttagagtcat
      241 gtttgcatga caacttgtag ctgcttattc cgatttccgg cgcatcgatt tcaatgcttc
      301 cgattgtagg gttgaaagct agtacataag gtaaaagcct gataaattct ttaattgcat
      361 ttgaggcttt ttctctctga atttgcgcgt ctaatctata agtaaatgta gtattgaaaa
      421 tttgtgggcc atgtgcaggg cttgttacag cctttctatt cttaatagct tgtaatgcgg
      481 tattctcaat atcaggaata agatcttttg ttgaagtagc actgcgatta attgtaaaat
      541 atgtcgaaga ataacttccg ttattttcta aaagtagaat ttcaacgtca acaattgtgc
      601 ttaacaggtg ggtagttaag aatcctgtac caaacttgcc tgttttacgt cttggcattc
      661 catcaatggc ctccttagag gttatgacgt tgattaaacc ccggatatcc aactcggtga
      721 atgcgttgcc agtatgggag aaaatcaacc tatttgaatc gagtgccatt ctaatgttca
      781 ccgtagttcc atctaagctg ctgtccttgg cattttggag taattcccac gcccatcgtc
      841 caacactgaa tttatcttca gctggtagct cactcaatcg ttgatgaatt ttgtcagcga
      901 ttgagcgtag accatcctct ttgaagcctt caattaatgt gtcgctcatt agatttgatt
      961 ttcttaatta agaatttcct ttcaaatttt gtctattttg ggttgcgagc aagtaaaata
     1021 cataaaataa acctgcatta gcaacagtat gtttttttga gtataggctt catttaacat
     1081 tatgatctac tttttgtcgc ggttataaac attaattgag tcataataaa gtgaattgtt
     1141 ggatattaaa tactttaatt agtcattata ttttcgtttt tgtaacacat ccatatttat
     1201 aaggagtata aatataaact atgattttca tgaagcttga attttaatgc attgttgata
     1261 ttaaaaaata tcaatgaatg atttatttta caattaaatg ctcttttact atataaatgg
     1321 attccaaacc atttgaaaat ttacgagcaa ctccaagtat aaattcattt attttaatag
     1381 gttttgcctt caaatacact tcatcaacat aaacaacata ttgtttgcat tgatttttct
     1441 tatccggttt ttcacggatt ttaaattcta ttattttctt tccaaaaagc tcgcctaata
     1501 aagctttttc aagaatagac cctttttgaa caccaaatac tttatgaata ttgatatttt
     1561 tattgatatt cactactgag cctgaaatat cttcctcttc tttgagaagc ttagcatcat
     1621 agatagttaa cttattgtta tgtttaccta attcttcttc tatcgtctcc atcgccaaat
     1681 cgtcaattat gaagtcctgc tgttttgcat aatccattat ccgcctgcga atttcaatgt
     1741 ctgtaaggta ttcagaacct cgaaaaagct ctatcgcttc tactattgct tgtttcctgc
     1801 aaaaatggat aggccatggt gaacctaggc tgtcaaagta tactttgctg ccacaattgc
     1861 agaaaaaaaa gaatactggc tgattacatg aaggacatgt tgttggaaat gcctttgtat
     1921 agcaatatat tccgtgagtt gggatcatat gttatttagt aagcaccctt gtgccttctt
     1981 tttgcatcaa cgtattaaat tgtttccaag ataatctacg gctatactga ttatttgctg
     2041 gcttccattc aattgcacca tgactaattt ttaatgtgcc aattttgccg tcatcgtcgc
     2101 tgatggagaa tacagcgtcg gctttgccta attcactttt tggaattttg aaatttacat
     2161 aatgatttgc cataaaattt aattttagtg tttagaaatt atttaattac tactaagttg
     2221 aatatttcgg tcatactgca ccaccaattc cggaccaatg tgatccactg at
//
LOCUS       DAORKW010000581         2271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2899_length_2271_cov_4.445397, whole genome shotgun sequence.
ACCESSION   DAORKW010000581 DAORKW010000000
VERSION     DAORKW010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2899_length_2271_cov_4.445397"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PLB46_15730"
     CDS             <1..829
                     /locus_tag="PLB46_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRF78030.1"
                     /translation="VGQGIGMAMRGMRPIAEIQYLDYLLYGLQPISDDLATLQYRTAG
                     GQKAPVIIRTRGHRLEGIWHTGSPMGMIVHACRGVHVCVPRNMTQAAGMYNLLLNCDE
                     PAIVIECLNGYRLKEPLPTNISAFTVPLGVPEILTQGDDITVVSYGSTLRIVQDACKL
                     LVERGISAEIIDVQTLMPFDIYGIIRESVKRTSRVIFIDEDVPGGATAYMMQQVFKDE
                     SLFRYLDAFPKCLSARDNRSAYASDGDYFCKPNEEDIFEAIYSMMHDVSPKSHPAIF"
     gene            1050..1244
                     /locus_tag="PLB46_15735"
     CDS             1050..1244
                     /locus_tag="PLB46_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRF78031.1"
                     /translation="METGKIKFFNATKGFGFIVKDGTNEEIFVHQSGLIDKVKQNDLV
                     TFDIVDGRKGKNAVNVKIVG"
     gene            complement(1312..2031)
                     /locus_tag="PLB46_15740"
     CDS             complement(1312..2031)
                     /locus_tag="PLB46_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRF78032.1"
                     /translation="MGRAFAYRKEKKFKRWDAMAKNFTRIGREIAIAVKNGGPNPDTN
                     PKLRLAIQNAKSVQMPKDRVDSAIKRASSKEEGNFEEVVYEGIGPGGVAMMVECATDN
                     TTRTVANVRLLFSRGGGALGTSGSVGYLFDRKGIFKIEATKINLEEMELDLIDAGAED
                     IYEEEGVVYVETTFNDFGTMQKKLDDMKIEIMGAELLRLPHDKIKLNADNENLVNNLI
                     EKLEEDDDVVNIFHNMDEAEN"
BASE COUNT          670 a          438 c          451 g          712 t
ORIGIN      
        1 agttggccag ggtattggta tggccatgcg tggtatgcga ccaatagcag aaattcaata
       61 tttagattat ttattatatg gattacagcc aatcagcgat gacctggcaa ctttacaata
      121 cagaacagca gggggacaga aagcacctgt aattatcaga accagaggac atcgtttaga
      181 aggtatttgg cataccggct ctccaatggg tatgattgtg cacgcttgtc gcggtgtaca
      241 tgtttgtgtg ccgagaaata tgactcaggc tgccgggatg tataatctgc tgttaaactg
      301 tgatgaaccg gctattgtaa ttgagtgctt aaatggttat cgtctgaaag aaccacttcc
      361 aacaaatatt tctgcattca ctgttccttt aggtgttcct gaaattttaa cgcagggaga
      421 tgatattacg gtggtatcct atggttctac attacgtatt gttcaagatg catgcaagtt
      481 gctggtagaa cgcggaatta gtgcagaaat aattgatgtt caaacactta tgccttttga
      541 tatttatggt attattcgcg agtcggtaaa acgcactagc cgagtgatat ttatagatga
      601 agatgttccg ggcggagcaa ctgcttatat gatgcaacaa gtatttaaag atgagagctt
      661 gttcaggtat ttggatgcat ttcctaaatg tttgagtgca cgtgataatc gctcggctta
      721 tgctagtgat ggcgactact tttgcaaacc gaatgaggag gatatttttg aggcaatcta
      781 cagcatgatg catgatgtga gccctaaaag tcacccggct atcttttaat taaacgtaaa
      841 tgccagggta aaaaaacctg ccaattctga acgaaggaag gaagtggaac gggctgtaat
      901 acgttgatat tagggcgttt cagtcaattt tccatcccga atttccaagc acggtgctta
      961 caaaagtcac caaaaatatt ttgagataga tgcattatgt tatagttttg catcgttaaa
     1021 caatttatta attattattt ttttttaaaa tggaaactgg taaaattaaa tttttcaatg
     1081 ccaccaaagg ttttggtttt attgttaaag acggaacaaa cgaagaaatt tttgttcatc
     1141 aatcaggttt gattgataaa gtaaaacaaa acgacctcgt tacattcgac atcgttgatg
     1201 gccgtaaagg taaaaatgct gttaatgtaa agattgttgg ataataacaa cacacagcaa
     1261 agaaataaaa aaccctgacg attgtcaggg ttttttttat gctcgaatta tttagttttc
     1321 agcttcatcc atgttatgga aaatattcac cacatcatca tcttcttcta atttttcaat
     1381 caggttattc accaaatttt cattgtcggc atttaattta attttatcat gaggtaagcg
     1441 taataattca gcacccatga tttcaatttt catatcatcc aactttttct gcatggtacc
     1501 aaaatcgttg aaagtagttt caacatacac cacgccttct tcttcgtaaa tatcttcagc
     1561 acctgcatca attaagtcga gttccatttc ttctaaattg attttagtag cctctatttt
     1621 aaagatgccc ttgcggtcga acaagtaacc aaccgaacca cttgtgccta atgcaccacc
     1681 accacggctg aacaataaac gaacattagc tacggtacgt gtggtattat cggtagcaca
     1741 ttccaccatc atggcaacac cgccgggtcc aattccttca tacactactt cttcaaaatt
     1801 gccttcttct ttgctcgaag cacgtttaat agcagagtct actctgtcct taggcatttg
     1861 aacagatttg gcattttgaa tggccaaacg cagcttagga ttagtatcag ggttaggtcc
     1921 gccatttttt acagcaatag caatttcacg tccgatacgg gtgaagtttt tcgccattgc
     1981 atcccagcgt ttaaattttt tctctttgcg atatgcgaag gcacgtccca ttggtaaagt
     2041 aattttgagg atgcaaattt aatttgttcg ggcacaattg ccaacttgaa accgatttcc
     2101 tgtcagaata ccacaaattt gtgtgccgaa acctcattgt cagctgtcag acggaagaaa
     2161 tatacgccac ttgccaaatg tgaaatgtcg atagggtaga tgtagctccc ttccccttta
     2221 atactttcct cgatgcgaaa taccacctgt ccgttaaggt ccacgaggtc t
//
LOCUS       DAORKW010000582         2268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2907_length_2268_cov_4.805242, whole genome shotgun sequence.
ACCESSION   DAORKW010000582 DAORKW010000000
VERSION     DAORKW010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2907_length_2268_cov_4.805242"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /locus_tag="PLB46_15745"
     CDS             complement(<1..1800)
                     /locus_tag="PLB46_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRF78033.1"
                     /translation="MAEAKKTTKKAKKSNVSFLKDERVKKTAGVMLILASFFIGIAFA
                     SYLLTWKQDQSLIWEHPWRQVFNSSIAVQNHLGNLGAVVSHQFFYSWFGIASFFFVIW
                     FFTIGVNSFFAKRVFNNITIVGQGAVIMVWLSTMLGFVFYNAQFPYGGAFGKGTSFYL
                     ENTFGKIGAILMLTAAMIIYMIIFTKIDFSFLANMFRRKPKPETTDADPLSEDDMLVP
                     AMAVVEDKKENIIVPPVYTSNEEIKEPIIMELKKVDPVVEKQIPVTLEMDEPLTTVIA
                     GEEEPIMHEQDNLALDIEEPHEEIVELENMHYNFDPILDLPNYKYPTLELLEEYASET
                     ISINTEELERNKDQIIATLNNYNIQISKIKATIGPTVTLYEIIPAAGVRISKIKNLED
                     DIALSLAALGIRIIAPIPGKGTIGIEVPNVNKETVSMRSQIASDKFQHAKMDLPVCLG
                     KTISSENYIADLTRMPHLLMAGATGQGKSVGINALLISLLYKKHPSQLKFVMVDPKKV
                     ELSLFSVIEKHFLAKLPGEEDAIITDTRKVVATLNALCIEMDTRYELLKNAQVRNIKE
                     YNAKFVKRKLNPKEGHMFLPYIVLVIDEFADLIM"
BASE COUNT          688 a          544 c          353 g          683 t
ORIGIN      
        1 catgatcagg tcggcaaact catcaatcac caatactatg taaggcaaaa acatatgccc
       61 ttctttcggg ttgagcttgc gttttacaaa cttggcatta tactctttaa tattacgaac
      121 ctgggcattt ttgagcaatt cataacgcgt atccatttcg atacaaagcg cgttcaacgt
      181 ggctactacc ttgcgggtat cggtaataat cgcatcttcc tcgccgggta atttcgccaa
      241 aaaatgtttt tcaatcaccg aaaacaaact caattccacc tttttcgggt caaccattac
      301 aaacttgagt tgcgaaggat gcttcttata taataatgaa atcagcaatg cattaatacc
      361 aaccgatttt ccctgacccg ttgcaccggc cattaacaag tgtggcatgc gtgttaaatc
      421 agcaatataa ttttcgctcg aaatagtttt tcccagacaa acaggaaggt ccattttagc
      481 atgctgaaac ttatcgctcg caatttgact gcgcatcgaa accgtttctt tatttacgtt
      541 tggtacttca ataccaatag ttcctttacc cggaattggc gcaataatac gaatacccaa
      601 tgcggctaaa ctcagggcaa tatcatcttc cagattttta attttcgaaa tacgcacacc
      661 ggcggcagga ataatttcat acagcgtaac tgttggtccg atagtcgctt ttattttcga
      721 aatctgaata ttataattat tcagtgttgc aattatttgg tctttgttac gctctaattc
      781 ttctgtgttt atcgaaatag tttcactggc atattcttcc aataattcta aagtcggata
      841 tttataattc ggtaaatcta aaatcggatc aaaattgtag tgcatgtttt ccagttctac
      901 aatttcttcg tgcggctctt caatatctaa cgctaaatta tcctgctcat gcataatggg
      961 ttcttcttca cctgcaataa ctgtagttaa aggttcatcc atttcaagtg taaccggaat
     1021 ttgtttttca acaacagggt ccactttttt taattccatt ataatcggct ccttaatttc
     1081 ttcattcgaa gtataaacag gaggaactat tatattttct tttttatctt caaccaccgc
     1141 cattgccgga acaagcatgt catcctccga aagcgggtct gcatcagtag tttcaggttt
     1201 tggtttgcgg cggaacatat tcgctaaaaa actaaaatca attttggtaa atatgatcat
     1261 gtaaataatc atagccgcag taagcattaa aatagcacca attttaccga aggtattttc
     1321 taaataaaat gaagtccctt ttccaaaagc accaccgtaa ggaaactgcg cattataaaa
     1381 aacaaatcct aacatagtgc tcagccaaac cataattacg gcgccctgac caacaatagt
     1441 aatattatta aatacacgtt tggcgaaaaa actatttaca ccaatagtaa aaaaccaaat
     1501 cacaaaaaag aaacttgcaa tgccgaacca gctgtaaaaa aactggtgac taaccactgc
     1561 accaagatta cctaaatgat tttgcacggc aatactgcta ttaaaaactt gccgccacgg
     1621 atgttcccaa attaaacttt ggtcttgttt ccaagtgagc aaataactgg caaaggcaat
     1681 tccaataaag aaactcgcca atatcagcat cacccccgct gtttttttaa cacgctcgtc
     1741 tttcaaaaac gacacattgc tcttttttgc ctttttagtt gtttttttcg cttctgccat
     1801 cctataataa tatatacact aagtcgaagg taaaggtaca cttcccgccg gcacatgaaa
     1861 acgttatttt accccatttc cttatacggt tgtctaagat tttgggcgtt cccttccgca
     1921 ggtttttttt aaaaactaac caattaaatt cgccaaaccg acccctgaaa taagtcattc
     1981 cccatcatcc caattttcac ccaaaaaacc tgctccaggt cgggctatcc actccaactc
     2041 ctctcccgca gaaaaagcgg gatgcggggt ttccgttact atccctaacg cggttcagag
     2101 taaacattac ctatacaata tccaattcac ccacaaaaaa atccacttca atctgccaca
     2161 aagcaggtcc ttttctacgc accattccaa aaaatccgcg ccaatccgcc caatccgtgt
     2221 catccgcgcc tgtctgccgc caggcaggtt ccttttcaac cccccacc
//
LOCUS       DAORKW010000583         2259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2931_length_2259_cov_4.366606, whole genome shotgun sequence.
ACCESSION   DAORKW010000583 DAORKW010000000
VERSION     DAORKW010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2931_length_2259_cov_4.366606"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..465)
                     /locus_tag="PLB46_15750"
     CDS             complement(208..465)
                     /locus_tag="PLB46_15750"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HRF78034.1"
                     /translation="MKRITALNVLFTLVFVFICSSGFAQAGKTETGTASFYHDKFVGR
                     KTANGEIYSQEKLTAAHKSLPLGTWVKVTNLSNDSLVKRKV"
     gene            complement(552..938)
                     /locus_tag="PLB46_15755"
     CDS             complement(552..938)
                     /locus_tag="PLB46_15755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78035.1"
                     /translation="MLKQLLFALVTVGISFTVSAQEITFQGDATTKQEASGHVNARLG
                     LGQSLTISPVPLPQYDAKLQVTAVGVEYYSYVVRNAFGQIVELENLSGKPDSNYIDLN
                     GAVHQGIYFITFETNAGNVTRKFAVI"
     gene            complement(1022..1510)
                     /locus_tag="PLB46_15760"
     CDS             complement(1022..1510)
                     /locus_tag="PLB46_15760"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRF78036.1"
                     /translation="MPELITSYYNAPFGTLAIRHNEEFITAVQFLDEERTETHKPKGI
                     AQQCMFQLDEYFTGKLRNFDLPLGPSGTDFQMKVWKALQAVPYASTASYLDIAKLLGD
                     EKSTRAVGAANGKNPIAIIIPCHRVIGSDSSLTGYAGGLHRKKWLLQHEAKFGLGVQT
                     LF"
     gene            1604..>2259
                     /locus_tag="PLB46_15765"
     CDS             1604..>2259
                     /locus_tag="PLB46_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HRF78037.1"
                     /translation="MGLKSLLAKPFADYIAKRVYDAAKNAVANQEKIMKELVQTAAQT
                     QFGKDHHFSTISNYEEFKAAVPVRDYEALRFYIDKMVDGESDVLWPGKPLYLAKTSGT
                     TSGVKYIPLTKASIPNHINSARNALLMYVHETRNTDFISGKMIFLQGSPVLGEKNGIK
                     TGRLSGIVYHFVPGYLLRNRMPSYTINCIEDWETKVEAIAHETMSEKMSLISGIPPWV
                     L"
BASE COUNT          697 a          465 c          441 g          656 t
ORIGIN      
        1 taatagcatc ttcacttctg gcagacaatc ctaaaagcaa agcaaattcc cttcctcgtt
       61 tcaaagacaa ataaaacttt ccataccctc caaaaacagt ctcttgataa ttttgctgtt
      121 tttgaaattg agcatgaaac aatacatcca atcgttcaag taatttgaca gacggagtta
      181 aacttaaatt aaatcgaata ggtaaattta cactttccgt ttgactaaag aatcgttgct
      241 caggttggtg acttttaccc aagtacccag tgggagcgac ttgtgggctg cagtgagttt
      301 ttcctgactg taaatttcgc cattagccgt ttttctaccc acaaacttgt cgtgatagaa
      361 gctggcagtg cctgtctccg ttttacccgc ttgggcaaaa cccgacgagc aaatgaaaac
      421 aaaaaccagt gtaaacagga catttaacgc tgtaattctc ttcatgccga tgtgaaaatt
      481 agtaaacaca cctcttatac ccaaatttta ggccaggatg ttttccacat aggctcagaa
      541 agccaataaa atcagataac ggcgaacttg cgggttacgt tcccggcgtt agtttcgaag
      601 gtaataaagt agattccctg gtgaacggca ccgttcaaat cgatatagtt ggaatcaggt
      661 tttccactca gattttccaa ttctacaatt tgaccgaatg cgttgcggac aacgtaactg
      721 taatattcaa caccaacagc ggtaacctgt aacttggcat catactgtgg cagtggcacc
      781 ggtgaaatcg tcaacgactg acctaaacct aaccgcgcat ttacatgtcc cgatgcttct
      841 tgtttagtag tagcatcacc ttgaaaagta atttcctgtg ccgatactgt aaaagaaata
      901 ccaactgtta ccagagcaaa aagtaattgt ttgagcatgt tatagttatg gctgtgaatg
      961 aataataagc aaatgtattt ttttttaatg gtaattcaaa gttacaattg ttggaattgt
     1021 gtcaaaataa cgtttgcaca cctaatccga atttagcttc atgctgtaat aaccattttt
     1081 tgcgatgtaa acctccggca tacccggtta gtgaactatc gctcccaata acacggtgac
     1141 aaggtattat aattgcaata gggttttttc cattggctgc acctactgcc cgagtggatt
     1201 tttcatcgcc caataatttg gcaatatcaa gataactggc agttgaggcg taaggaacag
     1261 cctgcaacgc tttccaaacc ttcatttgaa aatctgtccc ggatggccct aatggcaaat
     1321 caaaattgcg cagtttaccg gtaaagtatt cgtccaattg aaacatacat tgttgtgcaa
     1381 tgccttttgg cttgtgggtt tcagtacgtt cctcgtctaa aaattgcacc gcagttataa
     1441 actcttcatt atgcctgata gcaagcgtac cgaagggagc attataatat gaagtaatta
     1501 actcaggcat aatttgcaca aatgtccgca taattatcgg aatagactaa aggacatcta
     1561 cccctaaaat gtaatttcga ttttatgcct ttatctttgc aacatgggat tgaagtcgct
     1621 gctagccaaa ccgtttgccg attatattgc aaaacgggta tatgatgctg caaaaaatgc
     1681 tgttgccaat caggaaaaaa ttatgaagga attagtgcaa acagctgcac aaacccaatt
     1741 cggcaaggat caccactttt cgactataag taactacgaa gaatttaaag ccgctgttcc
     1801 tgttcgcgat tatgaggcat tgcgatttta tatagataaa atggtggatg gagaatcgga
     1861 tgtgttatgg ccaggcaaac cattatatct tgcaaaaaca tcaggtacta cttcaggggt
     1921 gaaatacatt cccttaacca aagcttctat tcccaatcat attaactcag ccagaaatgc
     1981 cttactcatg tatgtgcatg aaacgcgcaa taccgatttt atttccggaa aaatgatttt
     2041 tttgcagggt tcaccggtat tgggagaaaa aaatggtatt aaaacaggcc gcctctcagg
     2101 aatagtttat cattttgtgc caggttacct attgcgcaat cgcatgccga gttataccat
     2161 aaattgtatc gaagattggg agacaaaagt ggaagccatt gcgcatgaaa ccatgtctga
     2221 aaagatgtcg ctcataagtg gtattccacc ttgggtgct
//
LOCUS       DAORKW010000584         2256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2941_length_2256_cov_4.443435, whole genome shotgun sequence.
ACCESSION   DAORKW010000584 DAORKW010000000
VERSION     DAORKW010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2941_length_2256_cov_4.443435"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1119
                     /locus_tag="PLB46_15770"
     CDS             271..1119
                     /locus_tag="PLB46_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78038.1"
                     /translation="MKTLKLAIFCFVLSSTCYSVFGGNSTSETITFTVNANGYTGLLK
                     LMQIGETQFDGKVYTFTFTPGDNYSCDLNTGSSFQDVITGRTSYIAIYFNKDHTINRV
                     VPESAATIINSNTGIVLNTTQITINPKRYKYSWYTTAEYNESLSHKYYTGKNTITLVN
                     GLTYQIGGGTDSELSHDPASNDLVSKYTNIFNFEVTESGTVSVYDHFNTNAKTKGNKL
                     IFKTSKVKIDPDEISGGKELTYISGSDTIIINKPTKLRVIRSTVGFVTWELAPGKTGY
                     YYFLPL"
     gene            1345..1971
                     /locus_tag="PLB46_15775"
     CDS             1345..1971
                     /locus_tag="PLB46_15775"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HRF78039.1"
                     /translation="MQCAPEELSENGYVYLDKMEHTNILPFISLYLKKKTTVVLLYNT
                     CLGIGLGCLIFWCWYCYNNFDLSIGQCLNYVSFGIALSFTFVPLHEFIHVLAYKWQGA
                     KHTAYDVNLKRFYFLAIADKFVANKKEFIIVALAPLGIISVVLILCLLFTNSLWTCTI
                     LAALLTHGLLSSGDIGLISYFQWHKNMQVVTYDDKAGQMTYFYGKSIQ"
BASE COUNT          749 a          364 c          367 g          776 t
ORIGIN      
        1 aaaaatattt taataatgcc aaaaatgtga tagttgctgt agatacgcag taccaatttg
       61 atacatggtt cggttacagc gaatttaatg cgatatattg ttacaatagc aaacacgaaa
      121 aaacgaaaag ctttaatact acacaaactg caacaaccct gttagaagct gttaaataaa
      181 ttggcaattc tgggtaaatt ggccctaacc cccaacatct aatacctttt aaacaataaa
      241 tatttatttt tcactcacaa ataaaaacct atgaaaactt taaaacttgc tattttttgt
      301 tttgtacttt caagcacctg ctattccgtt tttggcggca attcaacaag tgagaccatt
      361 acttttactg taaacgcgaa tggatacaca ggtttgttga aactaatgca aataggggag
      421 actcaatttg atggtaaagt atatactttc acttttacac cgggtgacaa ctacagttgt
      481 gatctaaata ccggttcttc atttcaagat gtaataactg gtaggacttc atatattgct
      541 atttatttta ataaagatca cacaataaac cgtgtggttc cggaatcagc tgcaacaatt
      601 atcaactcca acacaggaat tgttttgaat actactcaga ttactattaa tccaaaaaga
      661 tataaatatt cctggtatac taccgctgaa tataatgagt ccctatccca taagtattat
      721 acaggaaaaa ataccattac attggtaaat gggcttactt atcaaatagg aggtgggaca
      781 gatagtgaat tatcgcatga cccggcaagt aatgatttgg tatcgaaata cactaatatt
      841 tttaattttg aagtaacgga aagtggaact gtatctgttt atgaccactt taatactaat
      901 gcaaaaacaa aagggaataa acttattttc aaaaccagca aagttaaaat agaccctgat
      961 gaaatatcag gaggtaagga attaacttat atatctggtt cagatacaat aatcatcaat
     1021 aaaccaacca aattaagagt aattcgctca actgttgggt ttgtaacctg ggaacttgca
     1081 ccaggtaaaa caggttatta ttatttttta ccactctgat agaaccaaca atgtgttcta
     1141 aatttgcttt ttactacttg cgataaaaat gttttttacc tgactttttg ttaatagtag
     1201 ttgaaataat tatgaactca aatgttggat aactgtaaat cctaatcttg ataaaggtat
     1261 ttttacattt taacaagctt taaggtgcct ttttaacatt tcatcagtca ttcacttatt
     1321 tttgagtttc aaattttact aaatatgcaa tgtgcacctg aggaattaag tgaaaatgga
     1381 tatgtttatc tcgataaaat ggagcatacc aatatcctgc cgttcatttc attgtatttg
     1441 aaaaaaaaga caacggtggt actgctgtat aatacttgtt tgggaatagg attaggctgt
     1501 ttgattttct ggtgctggta ttgttataat aattttgact taagtatcgg acaatgttta
     1561 aattatgtat cgttcggaat tgcactttcc tttacatttg ttccattaca tgaatttatt
     1621 catgtgcttg cttataaatg gcaaggagcg aaacacacgg catacgatgt taatttaaag
     1681 cggttctatt ttctggctat tgccgacaaa tttgttgcca acaaaaaaga atttatcata
     1741 gttgcactgg ctcctcttgg tataatttca gttgtgttaa tcttatgcct tttgtttaca
     1801 aattctttat ggacctgcac gatacttgct gcgctcctta ctcatggcct attgtcatca
     1861 ggtgatattg gtcttataag ttactttcaa tggcataaaa atatgcaagt ggtgacttac
     1921 gatgacaaag caggccaaat gacctacttt tatggcaaat caattcaata aggcatacgt
     1981 gtttaggcga attgattcaa aactaattag gataatttgt ttcgtttgca tattgctttg
     2041 actgcaatgg ctatcatttt tcgactaaaa tcagtatatt tgttataccc actgtttaat
     2101 atattaaaaa ttttaatatg caaacatcga cactattttg caagactaat gcattttatc
     2161 tttattatgt ctctcatttg aatgtctcct ctttgcctcg atattttggg ggattaataa
     2221 agataaaatg cattagtctt gcaaaatagt gtcgat
//
LOCUS       DAORKW010000585         2253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2948_length_2253_cov_5.259327, whole genome shotgun sequence.
ACCESSION   DAORKW010000585 DAORKW010000000
VERSION     DAORKW010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2948_length_2253_cov_5.259327"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PLB46_15780"
     CDS             <1..1067
                     /locus_tag="PLB46_15780"
                     /inference="COORDINATES: protein motif:HMM:NF026974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78040.1"
                     /translation="ILKYSNKSIYLHTSLPMNITFPLNSSDPPFGMVMPGRNWAAATA
                     SGYGFGFNGQEIDTEIHSGSQTAIYWQYDNRLGKRWNVDPVFRAFISQYACFSNNPIT
                     RVDPKGDDDYFDIKGNYIGTDRNGSHFIKVVTEVDVQKQLYLDYETKTVDPSAVLDYC
                     SLTDYSYNFRRFESWTRLNRILNHYTGEDIYPEEYSNNESPASTKGKVIRIHIFKGEL
                     LKGMNNYHILKSTINDHEKLHKTDNEKGIQGSYERHTEIYLHQISCESFKLCNDIDFQ
                     QGIVASGVKYAVNSFFQENTKSREQWQKEFNDALQNNNINLQISISGGNSFKSASFTI
                     TNTETGAQSIYMVKKKSDPH"
     gene            1067..1510
                     /locus_tag="PLB46_15785"
     CDS             1067..1510
                     /locus_tag="PLB46_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78041.1"
                     /translation="MKYIKLSEIFFVAIFFLNCEKKSNNVVSNKQGQCWDYIVMNSKK
                     INPVHGICLKDDGSAFYYTNDFEDSIRYINDRVNDADPGGLTNVFFTWKILPKNETII
                     LDAFKGRLIHFSADSIVYLNGENNRNVLVPSNQSFKENWDVLNRP"
     gene            complement(1689..>2253)
                     /locus_tag="PLB46_15790"
     CDS             complement(1689..>2253)
                     /locus_tag="PLB46_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRF78042.1"
                     /translation="PAKLKRCKDIMVFGGTVGLRYSDLMALTKKNIETVYDTKYLKMR
                     SKKTGTNTSIKLPEYACAIIHRYRGLKTLLPQVSLAQMNDNMKLLCEKAGWTQEVGKL
                     RSKRGVVKKVTDKGKPYRFCDLVSTHIMRRTSITTLLSLGMPESMVHKISGHAAGSKE
                     FYKYVHYAQQFLDKETDMVFDKLAMKK"
BASE COUNT          764 a          369 c          387 g          733 t
ORIGIN      
        1 ttatactaaa gtattccaat aagtcaatat atttacatac atcattgcca atgaatataa
       61 catttccttt aaattcatct gaccctccgt ttgggatggt aatgccggga aggaattggg
      121 cggctgcaac cgcaagtggg tatgggtttg ggtttaatgg gcaggaaatt gatactgaaa
      181 ttcattcagg tagccaaaca gctatttatt ggcagtatga taatcgttta ggtaaacgat
      241 ggaatgttga tccagttttc agagctttca tttctcaata tgcctgcttc tctaacaatc
      301 caattacaag agtagaccca aaaggcgatg acgattattt tgatataaaa gggaattata
      361 tagggacaga tagaaatggg agtcatttta ttaaagtagt tacagaagtt gatgtccaaa
      421 aacaattata tttagattat gaaactaaaa ctgttgatcc atcagctgtg ctcgattact
      481 gctcattaac ggattatagt tataatttta gacgtttcga atcgtggact aggttgaata
      541 gaatcttaaa tcattatact ggtgaagata tttatccaga agaatactca aataatgaat
      601 cccctgctag cacaaaaggc aaggtcatta gaattcatat atttaaagga gagttattga
      661 agggtatgaa taattaccat attttgaaat caacgattaa cgaccacgaa aaattacaca
      721 aaacagataa cgaaaaaggt attcagggta gttatgaaag acataccgaa atatatttac
      781 atcaaatttc ttgtgaaagt tttaaattat gtaatgatat tgattttcag caaggtattg
      841 tagcatctgg tgtgaaatat gcagtaaaca gtttttttca agagaataca aaaagcagag
      901 agcagtggca aaaggaattt aatgatgcat tacaaaataa taatattaac ctccaaattt
      961 caattagtgg aggaaatagt ttcaaatcag catcatttac aattactaat acagaaactg
     1021 gtgcgcaatc aatttatatg gtaaaaaaga aatcagatcc acactaatga aatatattaa
     1081 attatcagaa atattttttg ttgcaatttt ctttctgaat tgcgagaaga agtctaacaa
     1141 tgttgtttca aataaacagg gtcaatgttg ggattatatt gttatgaatt caaagaaaat
     1201 taaccctgtt catggaattt gtttaaagga tgacggttca gcattctatt atacaaacga
     1261 ctttgaagac tcaattagat acattaatga cagagttaat gatgctgacc caggtggttt
     1321 gactaatgta tttttcacat ggaaaattct acctaaaaac gagaccataa ttcttgatgc
     1381 tttcaaaggc agactaatcc acttttcagc agacagtatt gtttatttaa atggagagaa
     1441 caatagaaac gtcttagtgc ccagtaatca aagctttaaa gaaaattggg atgtgctgaa
     1501 tcgaccttag ttaaaaaccc cgtattcaaa cagacccggt tattttaagc aaacagactt
     1561 aagctatcca agcttgcaga ggaaattcgt acaaaccgct ttgctaataa agtaacgaag
     1621 cctatagatt tatagaggaa atataacgat ttctcaagca attatgtatt tctcttctac
     1681 ttttagcgtc attttttcat tgccaattta tcaaaaacca tatccgtttc tttatctaaa
     1741 aattgttggg catagtgcac atatttataa aattctttac taccggcagc atgccccgat
     1801 attttgtgaa ccatcgattc aggcatacct aaactgagta aggtagtaat tgaagtcctc
     1861 cgcatgatat gtgttgatac taagtcacaa aaacgatacg gtttgccttt atccgttacc
     1921 ttctttacta cacctcgttt acttcttaac ttacccactt cttgggtcca tcctgccttt
     1981 tcacacaaca atttcatgtt gtcattcatt tgagctaggc tcacttgtgg taataatgtt
     2041 ttaaggccac gatatctgtg tataattgca caggcatatt ccggtagttt gatgcttgta
     2101 tttgttcctg ttttctttga acgcattttt agatacttag tatcatacac cgtttcaata
     2161 ttctttttag ttaatgccat gaggtctgaa taccttaaac caactgtacc gccaaatacc
     2221 ataatatctt tacatcgttt caattttgca ggc
//
LOCUS       DAORKW010000586         2253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2949_length_2253_cov_4.178344, whole genome shotgun sequence.
ACCESSION   DAORKW010000586 DAORKW010000000
VERSION     DAORKW010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2949_length_2253_cov_4.178344"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLB46_15795"
     CDS             complement(<1..343)
                     /locus_tag="PLB46_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78043.1"
                     /translation="MRRGLSRLILFTIVLISVLLCTQGQNPAAVDKSPTNKVYFFELH
                     EDIGPSSVRIVDRALEEAQAMNADYIVMSLDSYGGLLDAGDTIHVKLLRCPIPVICYV
                     TNNAASAGALIA"
     gene            443..1279
                     /locus_tag="PLB46_15800"
     CDS             443..1279
                     /locus_tag="PLB46_15800"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRF78044.1"
                     /translation="MKRICVLILMLIWVWSVVGNSTVPGDSVGVKVMDGKTLMVYRVS
                     AGETAYAVSRKYGVPFKDIAAANAGVDMGGLKAGQEIFVPTNIAANTVTTEPVVVKKE
                     EKTVYPTQEKITPVVATENKEVIVKEASTTSNEVPVEPTNNTSVLSNTDLSKEPAAAS
                     NNKSKSFAQLYAEYLSGEMIAASEKGVATWIENNTIETSGDRFYVLHNSAPVGSIVKI
                     RNLMNNRTIYAKVIGNLSESEVQEKVLVKLSSGAAERLNVLDNRFVVEITYYLPDEKA
                     MK"
     gene            complement(1334..1723)
                     /locus_tag="PLB46_15805"
     CDS             complement(1334..1723)
                     /locus_tag="PLB46_15805"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRF78045.1"
                     /translation="MAIVRLTTCYDHFESHRIVDVLESEGIQCFVTNENFSTLLPIYN
                     GMLGSGIQVMIDETDLEKAQVLLEEIQPKEVLVCPYCESNNIAFGLGVERNLKIAQIV
                     TSLLTLVPVGRNSFTYYCKDCKKDFKK"
     gene            complement(1769..>2253)
                     /locus_tag="PLB46_15810"
     CDS             complement(1769..>2253)
                     /locus_tag="PLB46_15810"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRF78046.1"
                     /translation="ILLSLIGGPSAVETYMQAIGVNAVQIRVNEHDMHQHPDTLYANW
                     TKLDAAATLLQLVFSGKILKTNTNNFLLETLYATTTGPLRLKGQLPEGTAVAHKTGTA
                     GEDNNGVTAAVNDIGIITLPNGNHLIICVFVRNSKEPFETNEKIIADISYAAWLYFKD
                     "
BASE COUNT          716 a          405 c          457 g          675 t
ORIGIN      
        1 tggcaataag ggctccggca ctggctgcat tgttggtaac ataacaaatt accggtatcg
       61 gacagcgcaa aagctttacg tggatggtgt ctccggcgtc cagtaaaccc ccgtaactat
      121 ccagactcat tacgatgtaa tcggcattca tagcctgggc ttcctccagc gcgcggtcca
      181 ctattctaac cgatgatggc cctatatctt catgtaattc aaagaaatag accttgttgg
      241 tcggtgattt atccacagca gccgggtttt ggccctgtgt gcatagtagt acactaatga
      301 gtacaatggt gaataaaatc aatctggata agcctctacg catgatttac aggtttactt
      361 ttgcatgtga ataatttgtt aataaacaat agctccacaa aaatacgcgt taattatgga
      421 agcactttta caagataaaa atatgaagag aatttgtgta ttaatactca tgctcatttg
      481 ggtatggagt gtagtaggaa acagcacggt tccgggcgat tcggtaggtg ttaaagtaat
      541 ggatggcaaa accctgatgg tttatcgtgt gagcgccgga gaaacagcct atgcggtgag
      601 cagaaaatat ggtgtgcctt ttaaagatat tgccgccgcg aatgccggtg tcgatatggg
      661 tggattaaaa gcaggtcagg aaatttttgt tccgaccaat attgctgcca acacagtaac
      721 cactgaaccg gtagtggtga aaaaggaaga aaaaacagtt tatcctacac aggaaaaaat
      781 tactccggta gtggcaacgg aaaataagga agtaattgtg aaagaagcat cgaccacatc
      841 gaatgaagta ccggttgaac cgaccaataa tacttccgtt ttgtcgaata ctgatttaag
      901 caaagaaccg gcagcagcga gtaacaataa atcaaaatcg tttgcacagt tatatgccga
      961 atatttatcc ggtgaaatga ttgctgcttc agaaaaaggt gtagctacct ggattgaaaa
     1021 caatactatc gaaacatcag gtgatcgttt ttatgtatta cataattcag cacctgtagg
     1081 ttcaattgta aaaattcgta atttgatgaa taaccgcacg atatatgcaa aagtgattgg
     1141 taatttaagt gaaagtgaag tacaggaaaa agtacttgtt aaattaagtt caggtgcagc
     1201 agagcgatta aatgttttag ataatcgttt tgtagtagaa attacctact accttcctga
     1261 tgaaaaagcg atgaaataaa aattgttcgt taataagtgt gaaaagcgat gaggcgtgtc
     1321 ttgtcgcttt tttttatttt ttaaaatcct ttttgcagtc cttacaatag taagtaaaac
     1381 tatttctgcc tacaggaact aaggttagta aagaagtaac tatttgagca atttttaaat
     1441 tcctttcaac acctaaacca aatgcgatat tgttagattc gcaatagggg caaactaaaa
     1501 cttctttagg ttgaatttct tcaagcaaaa cctgcgcttt ctccaaatca gtttcatcta
     1561 tcattacctg aatgcccgag cctaacatgc cattgtaaat aggtaataat gtgctgaagt
     1621 tttcgttggt gacaaaacat tgaatgccct cgctttccaa tacatctact atgcgatgtg
     1681 attcgaaatg gtcgtaacaa gtggttaatc gcacaattgc catacaatgt agttaattac
     1741 taaagtaacg aaataataac catataaact aatccttaaa atataaccag gcagcataac
     1801 tgatgtcggc aataattttt tcgttcgttt caaatggttc ttttgaattg cggacaaata
     1861 cacaaataat gagatgattg ccattaggta aggttataat cccgatgtca tttactgctg
     1921 cagttacacc attattgtct tcacctgctg ttccggtttt atgagctacc gcagttcctt
     1981 caggtaattg gccttttaaa cgcagtggac ctgttgttgt ggcatataat gtttcaagta
     2041 aaaaattgtt tgtatttgtt tttaaaattt tgccggaaaa tactaattgc aacagcgtag
     2101 cagcagcatc cagttttgtc caattggcat ataatgtatc aggatgttga tgcatgtcgt
     2161 gttcatttac acgtatttgc actgcattaa caccaattgc ttgcatgtaa gtttccactg
     2221 ccgaaggtcc acctattaat gacaataaaa tac
//
LOCUS       DAORKW010000587         2251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2955_length_2251_cov_5.247268, whole genome shotgun sequence.
ACCESSION   DAORKW010000587 DAORKW010000000
VERSION     DAORKW010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2955_length_2251_cov_5.247268"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLB46_15815"
     CDS             complement(<1..533)
                     /locus_tag="PLB46_15815"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HRF78047.1"
                     /translation="MNLSIAYSPCPNDTFIFDALAHQKIDTEGLTFDIIHGDVETLNR
                     EAMDGKYDITKLSYHAFAYMSNHYQLLTAGSALGRGCGPLMISKDEIPRSKIEFCLIG
                     IPGRLTTANFLMTLAFPEAATKKEMVFNEIENALLNDAIDIGLIIHENRFTYIHKGLK
                     KIIDLGEWWEKKYQLPIP"
     gene            complement(530..1207)
                     /gene="mqnB"
                     /locus_tag="PLB46_15820"
     CDS             complement(530..1207)
                     /gene="mqnB"
                     /locus_tag="PLB46_15820"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HRF78048.1"
                     /translation="MKLLLVAATELELQPLLGEAKYMPGSVFQLLQKGNSSVDVLLTG
                     VGIASATFHITRLLSTKRYDLAIQAGIAGSFNKEIELGEVVIVANDRFAQLGVDDDQS
                     FLDVFELGFTQPNNYPFVNGSLEPSMPSLAFNSNFKLVRAITVETASGNDHAITKLVS
                     KYHPDIESMEGAAFYFCCMHMHLPCFQLRGISNYVEKRNKANWNIPLAVEQLDIALQK
                     IITNSIL"
     gene            1303..1920
                     /locus_tag="PLB46_15825"
     CDS             1303..1920
                     /locus_tag="PLB46_15825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HRF78049.1"
                     /translation="MRLVFILSLVLFAGLLNAQTEAIYFKADSLFTFKDSRDGKTYLA
                     FEINGVAWMGENLRHKTSESWCYEDKKANCDEYGRMYNWEEALTACPKGWHIPSRVEW
                     DSLIMWLGGDRKAGYSLAYSDSLGFNIKFGYPPNVNGRYSDANVQASYWTSDENSAST
                     AWVYYFIKQKLPFVYTNYFSKNYGMMCRCVQDNASNAVEESEIIK"
     gene            complement(1972..2042)
                     /locus_tag="PLB46_15830"
     tRNA            complement(1972..2042)
                     /locus_tag="PLB46_15830"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2008..2010),aa:Cys,seq:gca)
BASE COUNT          706 a          415 c          429 g          701 t
ORIGIN      
        1 ggtattggca attgatattt tttttcccac cattcaccta aatcaattat tttttttaat
       61 cctttgtgaa tataggtaaa tctgttttcg tgaataatta aaccgatatc gatagcatcg
      121 ttgagcagtg cattttctat ttcattaaat accatttctt tttttgttgc cgcttccgga
      181 aatgctaatg tcattaaaaa attagccgtt gttaatcgcc ctggaatgcc tattaaacaa
      241 aattcgattt tagaccttgg aatttcatct ttcgaaatca ttaatggccc gcaacctctt
      301 cccagagctg aacccgctgt aagtaattga taatgattac tcatataggc aaaggcgtgg
      361 taactcaatt tcgtaatatc atatttgcca tccattgctt cgcggtttaa tgtttccaca
      421 tcgccatgaa taatatcaaa agttaaacct tctgtatcaa ttttttgatg agcaagtgcg
      481 tcaaaaataa atgtatcgtt tggacaagga gaataagcta tactgaggtt cataaaatac
      541 tattggtaat gattttttgt aacgcgatat ctaattgctc aacagctaaa ggaatattcc
      601 agttcgcttt gttgcgtttt tctacataat tggaaatacc acgtaattga aaacaaggta
      661 aatgcatgtg catacaacaa aaataaaaag ctgctccctc catactttca atatcaggat
      721 gatattttga aactaattta gttattgcat gatcgtttcc acttgcagtt tctaccgtta
      781 tggcgcgtac taatttaaaa tttgaattaa atgctagtga tggcattgaa ggttcaagac
      841 ttccgtttac aaatggataa ttattaggct gagtgaaacc caattcaaaa acatccaaaa
      901 aagattggtc atcatcgacg ccaagttgcg caaacctgtc gttggcaaca attaccactt
      961 cacctaattc aatttccttg ttaaaacttc ccgcaatgcc tgcctgaatt gctaaatcat
     1021 aacgtttggt ggacaataat cgcgtaatat gaaatgttgc tgaagctatg ccgacaccgg
     1081 ttaaaagcac atcaacagat gaattaccct tttgtaacaa ctgaaaaaca gaaccgggca
     1141 tgtattttgc ctctcctaat aaaggttgca gttcaagttc ggttgcagct actaaaagca
     1201 atttcataat gcgaaggtag cctaaatttc accttaccga aaaggaacct gcacaaatgt
     1261 taattaattt tgtgaccttt atactgactt tataagcaca caatgagact agtatttatt
     1321 ttgagccttg tattatttgc cgggttatta aatgcccaaa cggaggctat ttattttaaa
     1381 gcagattccc tttttacctt caaagacagc agagatggta aaacatattt ggcatttgaa
     1441 attaacggtg ttgcctggat gggagagaac cttcgccata aaacaagtga aagctggtgt
     1501 tatgaggaca aaaaggcgaa ttgtgacgaa tatggcagaa tgtataattg ggaagaagcg
     1561 ctaactgcat gtcctaaagg ttggcatatc ccttctcgag tggaatggga tagtttgata
     1621 atgtggctag gtggagatag aaaagcggga tattcattgg cctacagcga tagtttgggt
     1681 tttaacatca aatttggtta tccgccaaat gtaaatggcc gttatagcga tgcgaatgta
     1741 caggcaagtt actggaccag cgatgaaaat agcgcgtcta ctgcctgggt atattatttc
     1801 atcaaacaaa aattaccttt cgtttatacc aattattttt ctaaaaatta tggtatgatg
     1861 tgccgctgcg tgcaggataa tgcatcaaat gcagtggaag aatcggaaat tattaaataa
     1921 gacaatggcg ttttagggca aaaaaaaatc cgctgaattt caacggattt gaggtcggaa
     1981 gcggattcga accgctgtag aagcttttgc agagccttgc ctagccactc ggccatccga
     2041 ccattgaggc gcaaaaataa aaacttatta cataactcgc taatttaaat tttcgttccc
     2101 gcatctttta tttctataca caccttttga atgtcgccac caagcggtgg atttaattta
     2161 caaatgcgga tagtaatatt ccccgaattg ccaatgaagt tgcgggtgcg aagcacaatt
     2221 tgccgtgctg cagtttcgat aagctgatat c
//
LOCUS       DAORKW010000588         2248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2963_length_2248_cov_4.492476, whole genome shotgun sequence.
ACCESSION   DAORKW010000588 DAORKW010000000
VERSION     DAORKW010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2963_length_2248_cov_4.492476"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..>2248)
                     /locus_tag="PLB46_15835"
     CDS             complement(402..>2248)
                     /locus_tag="PLB46_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRF78050.1"
                     /translation="GLALRSIQRKVSRVLFDKRIIMLDLAALVAGTKYRGQFEERMKA
                     IMNELEKNRDVILFIDEIHTIVGAGGATGSLDASNIFKPALARGELQCIGASTLDEYR
                     QYIEKDGALDRRFQKVLVDPPSPEETVEILTNIKSKYEEFHNVIYSTEAIKACVTLST
                     RYITDRFLPDKAIDVLDEVGARVHLKNIHVPDEIVKLEGQIEEIKEEKNKVVKSQRYE
                     EAAKLRDTEKRLLEDLDKEKTKWEEHVKSERYPVTEDDIAEVVAMMTGIPTKRIAQSE
                     GTKLVGMGDELRRGVIGQDEAILKITRAIQRNRVGLKDPKKPIGSFIFLGPTGVGKTE
                     LAKVLSEYLFDSKDSLVRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEKVRRKPY
                     SVILLDEIEKAHPDIFNILLQVLDDGMLTDGLGRKIDFKNTLIIMTSNIGARQLKDFG
                     SGVGFSTQAKQLAQDENNKGVIETALKRTFSPEFLNRIDDVVIFNSLSREDIFKIIDI
                     TLRDVKNRLETLNYKLQLEDSAKEFLSEKGYDPQFGARPLHRAIQKYLEDPLAESILS
                     GKFKEGDTISVEYDKDAAGLILNPAGEKEKKKKDKGKDKKEEKEEEKA"
BASE COUNT          645 a          444 c          387 g          772 t
ORIGIN      
        1 agcatgatga gtagatggaa taagttgatg tctatcagat aattcatttt ctaatttaaa
       61 catcttttgt tgagcagaaa catcaaaatg aaggatgagt atggcgacta aagtgaatag
      121 gaatttgggg ttcatatgtg tagactaagg taaagaaaaa tgcataaatg ttgaagggaa
      181 aatttatttg ttggtttttt ttggccaagc cgggtaagtg gcagcgcagg gttatttata
      241 gccctgatcc cgatagctat cgggagtgga aatcctttgc gcttttcagc aaagattgga
      301 acggaaagca ggggcgcggg ttgtgagaaa ctgcaagcta tgtgcttcaa aaaaaaatcc
      361 tgcaaataca ttgcaggatt ttaatttatt taaaaaacga cttaggcttt ttcctcttct
      421 ttttcctctt ttttatcctt gcctttatct tttttctttt tttctttttc tccggcagga
      481 tttaaaatta agcctgctgc atctttgtca tattcaacgg aaatggtatc accttcttta
      541 aatttaccgg aaagaatact ttctgcaagc ggatcttcca ggtatttttg aatggcacgg
      601 tgtaaaggac gtgcaccgaa ttgtgggtcg tacccttttt cacttaaaaa ttctttggca
      661 ctatcctcta actgaagttt gtaatttaaa gtttccagtc tgttttttac atcgcgtaaa
      721 gtgatatcga taattttgaa aatatcttca cgacttaatg aattaaatat taccacatcg
      781 tcaatacgat ttaaaaactc tggtgagaag gtgcgtttta atgcagtttc aattacacct
      841 ttattatttt catcctgggc taattgttta gcctgggtag aaaatcctac acctgaacca
      901 aaatctttta actgacgtgc gccaatatta gaagtcataa tgattaaggt atttttgaaa
      961 tcaatttttc tacctaaacc atctgtaagc ataccatcat ctaatacttg taataaaata
     1021 ttaaaaatat ccggatgcgc tttttcaatt tcatctaata aaataacgct atatggtttt
     1081 ctgcgtactt tttctgtaag ctgaccacct tcttcgtaac caacatatcc cggaggcgca
     1141 ccaattaatc ggctcacgct gaatttttcc atgtattcac tcatatcgat acgcaccagc
     1201 gaatcttttg aatcgaataa atattcactc aacacttttg ccaattcggt tttaccaaca
     1261 cctgtagggc cgaggaaaat aaatgaaccg attggttttt tcgggtcttt taatccaaca
     1321 cgattacgtt gaatggcgcg tgtaatttta agaatggctt catcctgacc aataacaccg
     1381 cgacgtaatt catcacccat acctaccaat ttggtacctt cactttgagc gatacgtttg
     1441 gttggtatac ctgtcatcat tgctactact tcggcaatat catcttcggt aaccggataa
     1501 cgttcacttt taacgtgctc ttcccatttc gttttttctt tatctaaatc ttcgagcaaa
     1561 cgtttttcgg tatcgcgcaa ttttgctgct tcttcgtaac gctgactttt tactacttta
     1621 tttttttctt cttttatttc ttctatctga ccttcgagtt ttacaatttc atcaggcacg
     1681 tgaatatttt ttaaatgcac gcgtgcacca acttcatcta aaacatcaat cgctttatcc
     1741 ggaagaaaac ggtcggtaat atatcttgtg cttaaagtaa cacaagcttt aattgcttca
     1801 gttgaataaa ttacattgtg aaactcttcg tatttcgatt tgatattagt aagaatttca
     1861 actgtttcct caggtgaagg cggatcaacc aatacttttt ggaaacgacg gtctaaagca
     1921 ccatcttttt caatatactg acgatattca tctaaagttg aagcaccaat acattggagt
     1981 tcaccacgtg ctaaagccgg tttaaatata ttagatgcat cgagagaacc tgttgcacca
     2041 cctgcgccga caattgtatg aatttcatcg ataaataaaa tcacatcgcg atttttttcg
     2101 agttcgttca taattgcttt catacgttcc tcaaactgac cacggtattt tgttcctgca
     2161 accaatgctg caaggtcgag catgataata cgtttgtcga ataacacacg tgataccttg
     2221 cgctgaatac tacgcaatgc caatcctt
//
LOCUS       DAORKW010000589         2246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_2969_length_2246_cov_3.504336, whole genome shotgun sequence.
ACCESSION   DAORKW010000589 DAORKW010000000
VERSION     DAORKW010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2969_length_2246_cov_3.504336"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1792
                     /locus_tag="PLB46_15840"
     CDS             272..1792
                     /locus_tag="PLB46_15840"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HRF78051.1"
                     /translation="MNRRSFLRNTLAGTTAVAVTPAAFLASCGTDPVVPIFFSVGGVD
                     VPAPPFTTPFNTLPINSASSASLVANKNLANLIGITDTFCFGYSDSVWGPTLKIQKGN
                     PVSITLTNNVNETTNLHFRGLTVDAGNSDNVEDAISNGGSKTYNFTVNNRAGLYMYHP
                     HYQKNAGKQMNLGLGGLFLVEDSEETALNLPSGENRETTIVIQDKRFNEDNMMFYGPN
                     PGEQMAGYFGETILVNGVHGPFKNISTRIHRLRIANASTARIYNLGLTDLTTGLEIPF
                     TVIGGDCGLVKNTGGAINAVLLAPGERVDTLVDFTSIAVGTEVMMSSKVFSGGGDYQG
                     SVGFDVMKFVVDTNEAEGYTIPDSLSDLVELTEADVTGETRNLNISNGNISQDEASSN
                     TDSMHVISGETYDAGTVNYTVTAGTTEKWVFDNSEGKEPRAMHIYGVQFQILTREGGR
                     GNTKLWERGWKDTVLMLPGEKVTCIVPFTVGAGKYNVVCTNLEHSDSGLMHTFEVV"
     gene            1940..2140
                     /locus_tag="PLB46_15845"
     CDS             1940..2140
                     /locus_tag="PLB46_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78052.1"
                     /translation="MLLKQAIRPAKLTLLFCTLFLANLTLKSQSHSNGDMTQFYIVSI
                     VFVVLIVGGVMSFFVSNKKKKD"
BASE COUNT          712 a          384 c          438 g          712 t
ORIGIN      
        1 agaatacaga agcggttacc gacatgggac tacgtggaat gattccaagt tgggagtgga
       61 agtaagaaat tatttatata catttcaaaa aacttttaac attcaaataa aatattatct
      121 tagccccaag ttttgtgaaa caataacaac agccaagttt ttaaagttta aacctaaaag
      181 aacatcctat ctgataaatt tttgctatct ctaaaaaatg cacacacaaa acattaatct
      241 gataagttta accaaaaaat aacgacagaa gatgaacaga agatcatttt taagaaacac
      301 gcttgcgggt acaacagccg tagcggtaac accggctgct ttcctcgcca gctgtggaac
      361 tgacccagta gttccaattt ttttcagtgt tggcggagtt gacgttcccg cgccaccttt
      421 taccacccct tttaatacct taccaatcaa ttcagcatct agtgcttctt tagtagccaa
      481 taaaaattta gctaacctga tcggtatcac agatactttc tgttttggtt attccgattc
      541 tgtttgggga ccaactttaa aaattcaaaa aggaaatccg gtaagtatta cgcttactaa
      601 taatgttaat gaaactacca atttacattt tagagggtta actgttgacg ctggaaattc
      661 agacaatgtt gaagatgcaa tatctaacgg tggcagtaaa acttataatt tcacagtaaa
      721 taaccgcgca ggtttatata tgtatcatcc acattatcaa aaaaatgcgg gtaaacaaat
      781 gaacctaggt ttaggtgggt tatttttagt tgaagatagc gaagaaactg cacttaattt
      841 gccttcaggg gaaaatcgtg aaactactat tgttattcag gacaaacgtt ttaatgaaga
      901 caacatgatg ttttatggcc ctaaccccgg cgaacaaatg gcaggttatt tcggagaaac
      961 cattttagta aatggtgttc atggtccttt caaaaatatc tctacacgta tacacagatt
     1021 gcgtattgca aatgcaagca ctgctcgaat ttataattta ggtttgaccg acttaactac
     1081 tggattggaa attccgttta ccgttatagg tggtgactgt ggtttagtga aaaataccgg
     1141 tggcgcaatt aatgccgttt tattagctcc tggtgaacgt gttgatactt tagttgactt
     1201 tacatcaatt gctgtaggca ctgaagtaat gatgagttct aaagtatttt caggtggtgg
     1261 tgattatcag ggttcagttg gtttcgatgt aatgaaattt gttgtggata ctaatgaagc
     1321 tgaaggatac acaattcctg actcactttc agatttagtt gaattaacag aagcagacgt
     1381 taccggtgaa accagaaacc tcaatatcag caatggtaat attagtcagg atgaagccag
     1441 cagtaataca gactcaatgc atgtgatcag tggtgaaaca tatgatgcag gcaccgtaaa
     1501 ttacactgta actgcaggaa caactgaaaa atgggtattt gacaactcag aaggcaaaga
     1561 acctcgcgct atgcatattt atggtgttca attccaaatt cttactcgtg aaggcggacg
     1621 tggcaacact aaattgtggg aacgcggctg gaaagacact gtattgatgt tacctggtga
     1681 aaaagtaacc tgtatagttc catttacagt tggtgcaggc aaatacaacg tagtttgtac
     1741 caatcttgaa cattccgatt caggtttaat gcatactttt gaagtagtat aatcgtcatg
     1801 attatatgta tatcagaaac ccgccttgtg cgggtttttt ttatgttatg actgttttct
     1861 tacatttcag gacaatattc acatgggtat attgcgtttt gtggttaaat tgattacctt
     1921 agaaacacaa acctaatgta tgctgttaaa acaagccatc cgtcccgcaa aacttacctt
     1981 gttgttttgt actttgtttt tagccaatct aaccttaaaa tcacaaagtc atagcaatgg
     2041 agacatgact caattttaca ttgtttcaat tgtttttgtt gttttgattg ttgggggtgt
     2101 gatgtcgttt tttgtttcta ataaaaagaa aaaagattga ccaacaccct atttttgtgt
     2161 ttaaataaaa atatggttag gataggattt cttttggcaa ttttgccttg tttaggtatg
     2221 caattatcct gtgaaagctc cggtac
//
LOCUS       DAORKW010000590         2202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3062_length_2202_cov_4.773172, whole genome shotgun sequence.
ACCESSION   DAORKW010000590 DAORKW010000000
VERSION     DAORKW010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3062_length_2202_cov_4.773172"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PLB46_15850"
     CDS             <1..675
                     /locus_tag="PLB46_15850"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HRF78053.1"
                     /translation="TTKSKTNKEVYEKNYGKPWLTDDLGVEEKYHDASGEDVIGYPGT
                     PMPVITIEKPEVIQAYIFGFLPHWLSPEKLNEQRATFNARIETITQLATWRDAWKTSQ
                     RCLVCTNGFYEHNKREKRKMFIHLKDEEYFYYGGIFNNYINKQTGEIKKTMAIVTTTS
                     NSLVAEIHSRMPVMIQKGNEGLWMDKTADTRHILMQYSQPLSPNFMVMDYADDEPKKQ
                     VQGSLF"
     gene            complement(672..>2202)
                     /locus_tag="PLB46_15855"
     CDS             complement(672..>2202)
                     /locus_tag="PLB46_15855"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRF78054.1"
                     /translation="EDIGLYKFDILSQRGLGHIKEAVEIVKANKGIDIDITPVEQFKK
                     DPLLNQNLLKGNTIGCFYIESPAMRSLLNKLLCDNYLTLVAASSIIRPGVAQSGMMDE
                     YIRRHRFPEHRKKIHPVMGDIMPDTYGVMVYQEDVIKVAHYFAKLTLSEADVLRRGMS
                     GKSRSKDEFQRIKQKFHANCTDLNYSKELIADVWHQIESFAGYSFAKGHSASYAVESY
                     QSLYLKTYYPLEFMVAVVNNFGGFYTAELYLHEAKKAGAHIEAPCINKSNYTTCIYGT
                     VIYVGLIHITGLENNVAERILAERQANGPFIDFDDFMKRVAINLEQLEILITINAFRF
                     SGIKKRELLIEAYRRLNKSKKTQPAATLFHTGAPTPYQFPELNLDTREDAWDEIKILG
                     FPLSSPFDLIDDPLIKNRRLVRVRDFSKYVGATVEIVGYLIITKHTATKTGEHMYLGT
                     FLDQDGNWLDTAHFPASAKKYRFKGKACYLLKGTIQESFSAYTLIVNEMYHLKMWLPK
                     D"
BASE COUNT          703 a          428 c          379 g          692 t
ORIGIN      
        1 actaccaaat cgaagacaaa taaagaagtt tacgaaaaaa attacggcaa accgtggtta
       61 actgatgatt taggtgttga agaaaaatac catgacgcca gtggtgaaga cgtaattggt
      121 taccccggaa cgccaatgcc ggtaattacc atagaaaagc cggaagtaat tcaggcgtat
      181 atttttggtt ttttaccaca ttggttatcg cccgaaaaac tcaatgaaca acgtgctact
      241 tttaatgcgc gtattgaaac tataacacaa ctagccacat ggcgcgatgc atggaaaaca
      301 agtcagcgat gtttggtttg caccaatggt ttttacgaac ataataaacg tgaaaaacga
      361 aaaatgttta ttcatttaaa ggatgaggaa tatttttatt acggtggaat ttttaataat
      421 tacatcaaca aacaaacggg tgaaataaaa aaaacaatgg ctattgtaac cactacatct
      481 aatagcctcg tagctgaaat tcacagtcga atgcctgtta tgatacaaaa agggaacgaa
      541 ggtttgtgga tggataaaac agcagatacc cggcatattt taatgcaata cagtcagccc
      601 cttagcccta attttatggt aatggattat gccgatgatg aacctaaaaa acaagttcag
      661 ggaagtttgt tttaatcttt tggcaaccac atttttaaat gatacatttc atttacaatt
      721 agtgtatagg cactgaaact ttcctgaatc gtccctttta ataaataaca agccttacct
      781 ttaaaccggt atttttttgc agaagccgga aaatgtgcag tatccagcca attgccatcc
      841 tggtctaaaa aagtgcctaa atacatatgc tctccggttt tagttgcagt atgtttggta
      901 attattaagt agccaactat ttcgacagtt gcacccacat atttgctgaa atcgcgcacg
      961 cgaaccaatc gcctgttttt aatgagcggg tcatcaatta aatcaaaagg tgaactcagc
     1021 ggaaatccta atatttttat ttcatcccat gcatcctctc tggtatcgag atttaactca
     1081 ggaaactgat agggtgttgg cgctccggta tgaaataatg tggctgcagg ttgtgttttt
     1141 ttcgatttat ttaatctgcg atatgcttca atcagtaatt ctcttttttt aattccacta
     1201 aaacggaatg cattaatggt aattaatatt tctaattgtt cgagattaat ggctacgcgt
     1261 ttcataaaat catcaaaatc aataaatggg ccattcgctt gtctttctgc taatatacgt
     1321 tcagcaacat tattttccag tccggtaata tgtatcaatc ccacataaat aactgtccca
     1381 taaatacaag tagtgtaatt acttttattg atacaaggtg cttcgatatg ggctccggct
     1441 tttttagctt catgcagata taattctgcc gtataaaaac ccccgaaatt atttaccact
     1501 gcaaccataa attcaagcgg ataatatgtc tttaaataca aactctggta actctctact
     1561 gcatacgaag ccgaatgtcc tttagcaaaa gagtatcccg caaaactttc aatctgatgc
     1621 catacatcgg caattaattc tttactgtaa tttaagtcag tacaattggc atggaatttt
     1681 tgttttatac gctgaaactc atctttactg cgacttttcc cactcatgcc tctgcgtaaa
     1741 acatcggctt cgctcagggt taatttggcg aaataatgtg ctactttaat tacatcttcc
     1801 tgatacacca ttacaccata agtatccggc ataatatcac ccattaccgg atgtattttt
     1861 tttcgatgtt cgggaaaccg atgccggcga atgtattcat ccatcatgcc tgactgcgca
     1921 actccgggac gaataattga actggctgca actaaagtga gataattatc gcataataat
     1981 ttatttaata aactgcgcat ggccggtgac tcaatgtaaa aacaaccaat ggtatttcct
     2041 ttcagcagat tttgatttaa taaagggtct tttttaaatt gctcaaccgg tgtaatatca
     2101 atatcaattc ctttattggc ttttacaatt tcaacggctt ctttaatatg gcctagtccc
     2161 cgctgactca aaatgtcaaa tttatataaa ccaatatctt ca
//
LOCUS       DAORKW010000591         2196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3074_length_2196_cov_5.171415, whole genome shotgun sequence.
ACCESSION   DAORKW010000591 DAORKW010000000
VERSION     DAORKW010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3074_length_2196_cov_5.171415"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1273)
                     /locus_tag="PLB46_15860"
     CDS             complement(116..1273)
                     /locus_tag="PLB46_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HRF78055.1"
                     /translation="MKNKKLNAGLMLTAIMLLNASPIWAQTITTEAPRNWYLTDTTFW
                     ILIAVAAILFYVIYALAEVVIWGGQKKINEMKNKGGNLKSLVVLAITLLATSNLHAQA
                     AEVATTTAKSSMWNSDYLPLYILIAIEIGVISYLALMLMQLSKAEQIASGVKHETWIA
                     KLWDKWNYKVPIEREDELIIEDHDYDGIHELDNSMPPWLQYIFFFTIGFAIVYMWYYH
                     LGGGLTQEEEYEASVKKAEIEMAAYLSSAAASYDENTVILSTDASVLANGKTTFNNYC
                     ATCHRTDGGGDAGPNLTDDYWIHGGKINDLFKTVKYGVPGKAMAAWEGILNPKQIFEV
                     TNYIKSMHGTNPVNPKAPEGNMYVEGAAVTDTLSTPIDTLAKDTLSVAVVK"
     gene            complement(1286..1471)
                     /locus_tag="PLB46_15865"
     CDS             complement(1286..1471)
                     /locus_tag="PLB46_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HRF78056.1"
                     /translation="MNFMHYLSDIDGVTIYPIISLFIFFAFFIGLFYWVFKMDKSSID
                     EISNIPLDNNNPNHLNQ"
     gene            complement(1477..2160)
                     /gene="ccoO"
                     /locus_tag="PLB46_15870"
                     /pseudo
     CDS             complement(1477..2160)
                     /gene="ccoO"
                     /locus_tag="PLB46_15870"
                     /inference="COORDINATES: protein motif:HMM:NF011055.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
BASE COUNT          670 a          418 c          364 g          744 t
ORIGIN      
        1 aatgcaattc tattctgtcc ttttaattaa tatgcctgaa tattaattgt gactaaatgt
       61 cactaaaaaa cttaaaataa cgttatccgc aaattgcgga taacgttatt aaattttatt
      121 taactacagc tactgataag gtatctttag ctaaagtatc aatcggtgtt gaaagtgtat
      181 cagttactgc tgcaccttca acatacatat taccttccgg cgcttttgga ttaaccggat
      241 tggtgccatg cattgattta atataattgg ttacttcaaa tatttgttta ggatttaata
      301 tgccttccca ggctgccatt gctttgcccg gaacaccata tttcacggtt ttaaataaat
      361 cgttaatttt tccgccgtga atccaataat catcggttaa gttaggaccg gcatcaccac
      421 caccatcggt gcggtgacaa gttgcacaat aattattaaa ggtggttttt ccgttagcta
      481 aaacagatgc atccgttgaa agaataacgg tattttcatc ataactggct gctgctgatg
      541 aaagataagc tgccatttca atttctgctt ttttaacaga agcttcatat tcttcctcct
      601 gtgttaaacc accaccaagg tgataatacc acatatatac aattgcgaaa ccaatagtga
      661 aaaagaaaat gtattgcaac catggtggca tactattatc taattcgtga atgccatcat
      721 aatcgtggtc ttcaattatt aattcgtctt cacgttcaat tggcactttg tagttccatt
      781 tatcccacaa tttggcaatc catgtttcat gtttaacacc tgaagcaatt tgttctgctt
      841 tactcagttg cattaacatt aaagctaaat aactgataac cccaatttcg atagcaatta
      901 aaatataaag tggtaaataa tcgctattcc acatagagga ttttgcagtt gtagttgcca
      961 cttccgcagc ttgcgcatgt aaattagatg tagccaataa agtaatagcc aaaacaacca
     1021 gagattttaa attacctccc ttgtttttca tctcatttat ttttttctgg cctccccaaa
     1081 ttaccacttc tgctagggca taaattacat aaaataagat cgctgccact gcaattaaaa
     1141 tccagaacgt agtatcggtt aaataccagt tgcgtggtgc ttcagtagta atggtctgag
     1201 cccatattgg tgatgcattt aacaacatta ttgctgttag catcaggccg gcattgagtt
     1261 ttttattttt catctcaatg attatttatt gatttaaatg attagggtta ttgttatcca
     1321 aaggaatatt gctgatttca tcaatggatg atttatccat tttaaaaacc cagtaaaata
     1381 atcctataaa gaatgcaaag aagatgaata gtgatattat cgggtagata gtcactccat
     1441 caatatctga caaataatgc atgaaattca tacgatttat tttttagctt gattttgtaa
     1501 attggtttct gtattgatat ctactcctaa tctttgcagg taggcaatca acgcaactat
     1561 ttcggcggta ttaggtgatt caatttcttt gtctgctttc atatttgcta caataccatc
     1621 tgcttgtttc aaataatctt cagttgcaat ttgatcatat ccttcaggat aaggaacacc
     1681 caatttttgc atagctctga tttttgccgg tatcatgtca agattaactt ctgaatcatg
     1741 caaccatgga tacgcaggca taatacttcc tgatgaaacc tggcgtggat ctaacatgtg
     1801 catataatgc caggcatcgg tttttttcaa gtggccaaca ccttcacgag ctaaatccgg
     1861 acctgtacgt tttgaacccc attggaatgg atggtcataa acaaattcac cggctttgct
     1921 gtattcacca taacgcacaa cttcacttct gaaaggacga accatttgtg agtgacaggt
     1981 ataacaacct tctcttacat aaatatcacg accttgtaat tcaagtggtg tatatggttg
     2041 cacacttgaa attgttggga tgttagattt aattaagaaa gtaggtacta attctattac
     2101 accaccaata attaccatga ttaaagcaac aatcataaat tggattggtc ttctttcaag
     2161 tgggcgatgc cagaattcac cttttggttt attcta
//
LOCUS       DAORKW010000592         2196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3075_length_2196_cov_4.591780, whole genome shotgun sequence.
ACCESSION   DAORKW010000592 DAORKW010000000
VERSION     DAORKW010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3075_length_2196_cov_4.591780"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="PLB46_15875"
     CDS             complement(<1..795)
                     /locus_tag="PLB46_15875"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRF78057.1"
                     /translation="MSAAVSEKVIFIVGNSRSGTTMMRRVLGNHPHIYMLEELHFFEQ
                     LWSTEDKTNAISKESAITLASKLLFIQRDGYLSQMNANKHAVDAKKIVDTITEPIYPH
                     AVLNGFLFFETQQHGKSIPCEKTPQDVFYIAEILELFPGAKIVNMVRDPRGVMLSQKR
                     KWQRRKMGAGFMTKKEQRRLKINYHPITISKLWNSSIRAAARFKDHPKVHNMIFERMV
                     NDSETEIKALCHFLEIDFTPDMLMIPQVGSSNEADKPDAFGIKTERA"
     gene            complement(813..>2196)
                     /locus_tag="PLB46_15880"
     CDS             complement(813..>2196)
                     /locus_tag="PLB46_15880"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HRF78058.1"
                     /translation="SLCRTWIPSQGGPGTRFTFDATVQVPVGMMALMSAENPQQTSTD
                     GVYHFKMEQPIPSYLMSLAVGDLGFQSVGAETGVYAEKSFVAACAEELSDMQTMLEKA
                     EQLYGKYQWGRYDVIVLPPSFPFGGMENPRITFATPTIIAGDKSLVALVAHELAHSWS
                     GNLVTNATWNDFWLNEGFTVYFENRIMEAVYGKDYADMLQVLEYDGLVETVKDFGETN
                     PDTHLKLKLEGRDPDEGVSDIAYNKGAFFLRLLESKVGRAKWDAFINAYFTSNAFKVM
                     TTEAFVEYLNANLIEPNKAAYADVDINVWIYGPGIPANITKPISVRFDLVDAQVKKFN
                     EGAAANTLVTTNWTTHEWLRFIYQLPDNTSLEKMGALDKQFKFTGTGNCEIADAWYEL
                     SIKAKYTTAYAEMDEFLNSVGRRKFLMPLYKAMINTGQGELAKSIYAKARPGYHYVAQ
                     NSLDELVGTK"
BASE COUNT          648 a          458 c          347 g          743 t
ORIGIN      
        1 ggcacgttca gttttaatac caaatgcatc aggtttatcg gcttcattac tcgacccaac
       61 ctgcggaatc ataagcatat caggcgtgaa atcgatttcc aaaaaatgac ataatgcttt
      121 tatttcagtc tctgaatcat taaccatacg ttcaaaaatc atattatgca cttttggatg
      181 gtctttaaat cttgctgctg cacgaatact cgaattccaa agtttagaaa tagttattgg
      241 gtgataatta atttttaatc gacgttgttc ttttttggtc ataaatcctg cacccatttt
      301 tctgcgttgc catttacgtt tttggctcaa cattacgcca cgtgggtcgc gaaccatatt
      361 tacgattttt gctcccggaa ataattccag aatttcagca atgtaaaaaa catcttgagg
      421 cgttttttcg catggtatgg atttgccgtg ttgttgggtt tcgaaaaata agaaaccatt
      481 taatacagca tgaggataaa ttggttcggt gattgtatcg acgatttttt ttgcatcaac
      541 agcatgttta ttcgcattca tttgcgataa ataaccatcg cgctgaataa acaataattt
      601 ggatgctagg gttatagcac tttctttact gatggcattg gttttgtctt cagtgctcca
      661 caattgttcg aaaaaatgca attcttccaa catataaata tgtggatgat tgcccaaaac
      721 cctacgcatc atggtagttc cactgcgcga attaccaact ataaaaatta ctttttcaga
      781 cactgctgca ctcatttctt gtgtaaaatt aattatttag tgccaactag ttcgtctaat
      841 gaattttgtg caacataatg ataacctggt ctggctttag cataaatact ttttgccaat
      901 tccccttgtc ctgtatttat catcgctttg tacaatggca ttaaaaattt ccttcttcca
      961 acactattta aaaattcatc catttctgca taagctgtcg tatattttgc tttgatggat
     1021 aattcatacc aggcatcagc aatttcgcaa tttcctgttc ctgtaaattt aaattgtttg
     1081 tccaaagccc ccattttttc taatgaagta ttatcaggta attgataaat aaatctcaac
     1141 cattcatgcg ttgtccaatt ggtagtaact aatgtatttg ctgcggctcc ttcattaaat
     1201 tttttcacct gagcatcaac taaatcaaaa cgcacagaaa tcggtttggt aatgtttgcc
     1261 ggaatacccg gaccataaat ccaaacatta atatctacat ctgcatacgc tgctttgttg
     1321 ggttcaatta agttagcatt taagtattca acaaatgctt cggttgtcat tactttaaat
     1381 gcattactgg taaaataagc attaataaac gcatcccatt ttgcgcgacc tactttactt
     1441 tccaataatc gcaaaaagaa ggcacctttg ttataagcaa tgtcgctaac accttcatca
     1501 gggtcacgac cttctaactt taattttaaa tgtgtgtcag gattggtttc accaaaatct
     1561 ttaactgttt ctaccaaacc atcatattct aatacttgta acatgtccgc ataatctttt
     1621 ccataaacag cttccatgat tctattttca aaatatacgg taaaaccttc atttaaccag
     1681 aaatcattcc aggttgcatt agttactaaa tttccgctcc agctatgtgc taattcatgt
     1741 gctaccaggg caactaaact tttatcaccg gcaataatgg ttggtgttgc aaaagtaatt
     1801 cttggatttt ccataccacc aaatgggaaa cttggaggta atacaattac atcataacgc
     1861 ccccactgat atttaccgta aagttgttca gctttttcta acattgtttg catatcgctc
     1921 aactcttccg cgcaagcagc aacaaatgat ttttctgcat acacgcctgt ttcggcacct
     1981 acagattgga accctaaatc gccaacggct aaactcatta aataagatgg tataggttgt
     2041 tccattttaa aatggtaaac tccatcagtt gaagtttgct gaggattttc agcactcatt
     2101 aatgccatca taccaacagg cacctgcaca gtagcatcaa atgtaaatct tgttcccggc
     2161 ccaccttgag atggtatcca ggttctgcat aaactc
//
LOCUS       DAORKW010000593         2194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3082_length_2194_cov_4.802244, whole genome shotgun sequence.
ACCESSION   DAORKW010000593 DAORKW010000000
VERSION     DAORKW010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3082_length_2194_cov_4.802244"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..>2194)
                     /locus_tag="PLB46_15885"
     CDS             complement(96..>2194)
                     /locus_tag="PLB46_15885"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRF78059.1"
                     /translation="ARALDGSTTWKVCTSPTPGATNDGSAMYADYTDRPDMSVLGGFY
                     GGPVTVELTSTTPDGVIHYTLDGGEPKMSDPVYSAPITINATAVLKARVFSANPDLIP
                     GKLQFNTYFINVNHVLPVVSIAGDTLLELANGNQNLEPIGSIEIFGKDLDSKTRSYGE
                     LNSHGQDSWANDQRSIDWVSRDEFGYSNALKEKFFASSERDEFQRMIFRAAGDDNYPA
                     AHHSENEGSAHLRDDYIQMLAKNDGLALDVRTSERCIVYLNGEYWGVYSFREKTDDHD
                     FTDFYYNQGKYDIQYQMTWGDTWSEYGGEPTLNAWSDLRDLAEDHDLTIDSNYKKVTD
                     QLDIYSLIDYMCVNTSAVCKDWLVYNTAVWRGFNPEGTHQKWGYTLWDLDATFGFYIN
                     YSGVPEVGPTALPCDVDEYNPAFFGPGFFDGEGHVRLMNDLRTNAEFEQFYLSRYTDL
                     YNTTFSCENMLNSLDSMMAIIEPEMNQHADRWYGTYEEWYDNFSTLRTWVEDRCELLY
                     TGMSDCYELTGPYDVVFNVDPPEAGYLKVNTLTHHDLPWTGTYFGGMDNKLVAYSNNP
                     ALYVFDHWEATTATFTPGWDSTGTVISENDAITAVFRTPTGIENIEQIPLSVYPTVFD
                     DAVIINFELTEGAAVSFDLYSVNGEKIATKLVGNNVSGSQFISWDLTSYNLASGIYII
                     KFNADEHTQTIKVIKQ"
BASE COUNT          704 a          455 c          354 g          681 t
ORIGIN      
        1 acgccacccc ggtctcccga ccggtacaaa ttatacaaat aaaaaaagcg gcttgttaaa
       61 atgaagccgc ttttgaaatt ttatcttaaa ataaattatt gtttaatcac tttaattgtt
      121 tgtgtatgtt cgtcagcatt aaatttaatg atatatatac ctgatgcaag gttatatgac
      181 gttaaatccc aactgataaa ttgtgaacct gaaacattat taccaactaa ttttgtagca
      241 attttttcgc catttacgct atataaatca aagcttactg cagcaccttc tgttaattcg
      301 aaattaataa taaccgcatc atcaaaaaca gtaggataaa cgcttaatgg aatttgttca
      361 atattttcaa ttcctgttgg tgtgcggaaa actgcagtaa tggcatcatt ttcagaaata
      421 actgttccag ttgaatccca acctggtgta aatgtagcgg tggtagcttc ccaatggtcg
      481 aaaacataaa gtgcaggatt attactgtaa gcaaccaatt tattatccat acctccgaaa
      541 taagtgcctg tccaaggtaa atcatgatgc gttaatgtat ttacttttaa ataaccggct
      601 tccggtggat caacattaaa tacaacatca taagggcctg ttaattcata acaatcgctc
      661 ataccggtat ataataattc gcaacggtct tctacccaag tgcgaagagt agaaaaatta
      721 tcataccatt cttcataagt tccataccaa cggtcagcat gttgattcat ttccggttct
      781 ataatcgcca tcatactatc caatgaattc aacatatttt cgcagctgaa agtggtgtta
      841 tacaaatcgg tataacgcga taaataaaat tgttcgaatt cagcatttgt ccgtaaatca
      901 ttcatcagtc gcacatgacc ttcaccatca aaaaatccgg gaccgaaaaa cgctggattg
      961 tattcatcta catcgcatgg taaggcagtt ggtcctactt ctggaacacc ggaataatta
     1021 atataaaatc caaaagtggc atctaaatcc caaagcgtat aaccccattt ttgatgagtt
     1081 ccttcaggat tgaaaccgcg ccaaactgct gtgttataaa ctaaccaatc tttacaaaca
     1141 gcagaagtgt ttacgcacat gtaatcaatt aaactataaa tatccaactg gtctgttact
     1201 tttttataat tggaatcaat agtcaggtca tggtcttctg ctaaatctct taaatcgctc
     1261 caagcattca atgttggctc accaccgtat tcgctccatg tatcgcccca tgtcatttga
     1321 tattgaatat catatttccc ctgattataa taaaaatcgg taaagtcatg gtcatcggtt
     1381 ttttctctga atgaatatac accccagtat tcaccgttta aataaacaat acaacgttct
     1441 gaagtacgta catctaacgc aagtccgtca ttttttgcaa gcatctgaat ataatcatca
     1501 cgtaaatgtg cagaaccttc gttttcggag tgatgagctg ccggataatt gtcatcacct
     1561 gctgctctga aaatcatgcg ctgaaattca tcacgttcag atgaagcaaa aaacttttct
     1621 ttaagtgcat tactataacc aaattcatca cgactcaccc agtcgatact acgctgatcg
     1681 tttgcccagc tatcctgacc atgagagttt aattcaccat aagagcgcgt tttgctatct
     1741 aaatcttttc caaaaatttc aattgagcca ataggttcca aattttgatt accatttgct
     1801 aattcaagta atgtatcacc tgcaattgag actacaggta aaacatggtt tacattaatg
     1861 aagtaggtat taaattgcaa tttgcctgga attaaatcag ggtttgcaga aaaaactctt
     1921 gcttttaaaa cagcagttgc attaatagtt ataggtgctg aataaacagg gtctgacatt
     1981 tttggttcgc ctccatccaa tgtgtaatga attacaccat ccggcgtagt tgaagtaagt
     2041 tccacagtta caggaccacc gtaaaatcca cctaaaacac tcatgtcagg tctgtccgta
     2101 taatcagcat acattgctga accatcattt gttgcacccg gagttggtga tgtacaaact
     2161 ttccatgttg tacttccatc taaagctctt gcac
//
LOCUS       DAORKW010000594         2193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3085_length_2193_cov_5.740879, whole genome shotgun sequence.
ACCESSION   DAORKW010000594 DAORKW010000000
VERSION     DAORKW010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3085_length_2193_cov_5.740879"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /locus_tag="PLB46_15890"
     CDS             <1..1976
                     /locus_tag="PLB46_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF78060.1"
                     /translation="EDGFGLRPNIGLRGTGVERSSKITIMEDGVLIAPAPYADPAAYY
                     FPTIGRMKAVEILKGSSQIQYGPFTTGGAINFISTPILDDFAVRVHATAGSFQNKNLH
                     AYVSNQHKNIGYLVETFQYGSDGFKTLENGGNTGFDKEDYLVKLKLNTNPDVKMPQSI
                     LFKVGQTNEQSNETYLGITANDFAANPYSRYAGSQMDNIATEQHQYSATHHIDLSKNV
                     QLTTVAYATDFKRNWYKLDKVADSSGVKSSIANILESPEDYNNAYQIIDGNVNDLANT
                     LYVRANNRAYLSRGVQTELRYKKIAGMFKHDVSIGARLHTDEVDRFQWDDEYTMVDGI
                     MQLTKSGTPGTESNRIKSADAFAAYAQYQLSIKKWTITPGLRFEHISLAEIDYGKTDP
                     ERIGTDIIENNNTADVIIPGIGIQYAINTGTQIFTGIHKGFSPPGTNDATTPEESINY
                     ELGTRINRTVWQMEATVFVNDYSNLLGSDLAAAGGDGTGDLFNAGKVLSKGIEFQGTF
                     DAFATNKNAVISLPISLIYTFSQANFKSGFVSTFEDWGTVEQGDDFPYLANHQFTGII
                     AFSYRSLSAHAGVNYMSAMRMEPGQGEIPEALLIPARTLIDCSIQYQMHRYISVFASV
                     LNAANNTYIAAGRPAGLRPGMPRTFNVGLKLDI"
BASE COUNT          739 a          356 c          422 g          676 t
ORIGIN      
        1 aagaagacgg ttttggttta cgtccgaata ttggtttgcg gggaacaggt gttgagcgta
       61 gttcaaaaat taccattatg gaagatggag tattaatagc tccagctcct tatgctgatc
      121 ctgctgctta ttattttcca actatcggcc gaatgaaagc ggtagaaatt ttgaaaggta
      181 gtagtcaaat tcaatacgga ccttttacaa ccggtggcgc tattaatttt atttctacgc
      241 ctatacttga tgattttgct gttcgtgttc atgcaactgc cggaagtttt caaaataaaa
      301 atttacatgc ttatgtttcc aatcaacata aaaatattgg atatctggtt gaaacatttc
      361 aatatggcag cgatggattt aaaacgttag agaatggcgg taataccggt tttgataaag
      421 aggattattt agtgaaatta aaattaaata ccaatcctga tgttaaaatg ccgcaatcta
      481 tattgtttaa agtcggccag acaaatgaac aatcgaacga aacttattta ggcataacag
      541 caaacgattt tgctgccaac ccatatagca gatatgcagg ttcacagatg gataatattg
      601 caacagaaca acatcaatac tcagcaactc atcatattga tttaagcaaa aatgtgcaac
      661 ttaccactgt tgcctatgca actgatttta aaagaaactg gtataaactc gataaagttg
      721 cggatagctc aggtgttaaa tcaagcatag ccaatattct tgaaagtccg gaagattata
      781 ataatgccta tcagattatt gatggtaatg tgaacgattt agcgaataca ttatacgtga
      841 gagcaaataa tcgcgcttat ttatcgcgtg gtgtacaaac agaattacgg tataaaaaaa
      901 ttgcaggaat gtttaaacac gatgtaagta tcggcgcacg tttacacacc gatgaggtag
      961 acagatttca atgggatgat gaatatacga tggttgatgg tataatgcaa ttaactaaat
     1021 cgggcactcc gggtactgaa agtaacagaa ttaaatctgc agatgcattt gctgcgtatg
     1081 ctcaatatca gttaagtatt aaaaaatgga ccattacacc aggtttgcga ttcgaacata
     1141 tttcacttgc agaaattgat tatggtaaaa ctgaccctga acgtataggc actgatatta
     1201 tcgaaaataa taatacggct gatgttatta ttccggggat tggaattcaa tatgcaataa
     1261 atacaggaac acaaatattc acaggtatac ataaaggatt ttctcctccg ggcaccaatg
     1321 atgcaactac acctgaagaa agtatcaatt acgaattagg aacacgcatt aatcgcactg
     1381 tttggcaaat ggaagcaact gtatttgtaa atgattacag caatttattg ggttccgatt
     1441 tagctgctgc aggtggtgat ggtaccggtg atttatttaa tgcaggaaaa gtattatcaa
     1501 aaggtattga atttcaaggc acattcgatg cttttgctac caataaaaat gcagttatca
     1561 gtttacccat atccttgatt tatacttttt cacaggcaaa ttttaaatct ggatttgtca
     1621 gtacatttga agactgggga acagttgagc aaggcgatga ttttccttat ttagcaaatc
     1681 atcagttcac cggaatcatt gcattttcgt atcgttcgtt gagtgcacat gctggagtca
     1741 attacatgag tgctatgcgc atggagcccg gtcagggaga aattcctgaa gcactattaa
     1801 ttccagcccg cacattaatt gattgcagta ttcaatatca gatgcatcga tatatttctg
     1861 tatttgcaag tgtattaaac gcagcaaata atacttatat cgccgctggc cgtccggcag
     1921 gattacgccc gggtatgccg cgcacattta atgtgggatt aaaactggat atttaattat
     1981 ttagatttgc gattgataat tacgtaaatt aataaagtat aactttactt accttttaca
     2041 attaagtttt gttctgttaa tcgaataaaa taaaagttgt ttgttttcta aaaaacatca
     2101 tgaaaatata ttacatggtt tatcattaat atatttaagc tcaatatatc gtaattaaaa
     2161 gtacataaaa aaacgaggta taaaatgata cac
//
LOCUS       DAORKW010000595         2184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3104_length_2184_cov_4.002349, whole genome shotgun sequence.
ACCESSION   DAORKW010000595 DAORKW010000000
VERSION     DAORKW010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3104_length_2184_cov_4.002349"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PLB46_15895"
     CDS             complement(<1..1183)
                     /locus_tag="PLB46_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78061.1"
                     /translation="MKKLTFYAALLMLASNLFAQSVTVGTTTYGLQSNGPTRNLITVY
                     NDGKISTAWTGSTTLDGSYLDRGTFYNHFNGVSWGAAPTTREETVRTGFGEIVNIGTK
                     EVLISHNGTTLEVFRNGAIGSSTFTETTGSNLIVGLWPSIYCPEGTDDIYVICANAAA
                     NPTAINFSRSMDGGDTWAVLNSPLPFLTSAEGFGTLTGNSAQLVVVGNDVYVLYGTEY
                     TDVVLLHSPTKGNAGTWTSTRVIDFPIDNYTGAVGQISDYNGDFIADTINTNDGYYEM
                     IVTSDGTVHVWTGAYRLLDDDPLTLGWSYFPLVAGIYYWNSATGTVSLLNFFFDTNNV
                     DGFNDPFLGLGSATGGYLGVNLTSMSTVAYDATNNRMFLMYSMPIENTDLFGDPYNPY
                     AQ"
     gene            1479..1652
                     /locus_tag="PLB46_15900"
     CDS             1479..1652
                     /locus_tag="PLB46_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78062.1"
                     /translation="MENNEKQKYEIKLNKKWFLAGLAATAVCIWMVIFMSQFFWIALP
                     FVFTFLALSIDAL"
     gene            1717..>2184
                     /locus_tag="PLB46_15905"
     CDS             1717..>2184
                     /locus_tag="PLB46_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78063.1"
                     /translation="MKKLYLLIVLALCSNILLAQSNPSWFTYDGTPTAGRFDDIFFIN
                     DSTGWAVSSDGEIYKTVNAGKNWVLKLGESTYFRSVEFFDENVGFAGSLFGELYKTVD
                     GGDSWEDIGDLLPHVFTGICGLSVADDTSIYACGIWSGCAYIFKWTDRGETWSY"
BASE COUNT          660 a          427 c          416 g          681 t
ORIGIN      
        1 attgtgcata tggattatat ggatcaccaa ataaatcggt gttttcaatc ggcatcgaat
       61 acattaaaaa cattctgtta tttgtagcat catatgcaac tgtcgacatc gatgtaagat
      121 tcacaccgag atatcctcct gttgcagaac ctaaacctaa gaacgggtca ttaaacccat
      181 caacattatt ggtatcgaaa aagaaattga gtaaagaaac tgtcccggta gctgaattcc
      241 aataataaat gccggctact agcgggaagt acgaccaacc taaagttaat ggatcatcat
      301 ccaataaccg ataagcacct gtccaaacat gtacagttcc atcactggta acaatcattt
      361 cataataacc atcattcgtg ttaatggtat ctgcaataaa atctccatta taatcagaaa
      421 tttgacctac agcaccagta taattatcaa ttggaaaatc tataactcga gtgctggtcc
      481 aggttcctgc atttcctttt gtagggctat gtaataaaac aacatcagtg tattcggtac
      541 cgtaaagcac ataaacatca tttcctacaa ctactaattg tgcgctattg ccggtcaatg
      601 taccaaaacc ttcagcactt gttaaaaatg gtagaggaga attgagcaca gcccaggtat
      661 caccaccatc cattgatcgc gaaaagttaa tagcagtcgg gtttgcagca gcatttgcac
      721 atatcacata aatatcatcg gttccttcag gacagtaaat agacggccac aaacctacaa
      781 tcaaatttga tccggttgtt tcagtaaaag tagaacttcc aattgcgcca tttctgaaaa
      841 cctccaaggt tgttccattg tgagaaatga gcacttcttt agtgccaata tttacaattt
      901 ctccaaaacc tgttcttacg gtttcttcac gcgttgttgg agcagctccc catgaaacgc
      961 cgttaaaatg attgtaaaat gtgcctcgat caagataact gccgtcgagc gttgtggaac
     1021 cggtccaggc ggtagaaatt ttcccatcgt tataaaccgt tatgagattt cttgttggac
     1081 cgttggattg cagcccgtat gtagtggttc ctacagtaac agactgggca aagagatttg
     1141 acgcaagcat caatagagct gcgtaaaatg tgagtttttt cattgtatgt attttgaaat
     1201 tatatccaaa cttaatcaaa tagttaataa tctataaagt caatatgata agtggcctat
     1261 ttttatcaat aattgcttta tgccattacc attgtaatga aatttactgt gtaattgggc
     1321 agtcccaata gtggcctaat acaagctaat accttaccac atgtattagg atgaaatttg
     1381 catgcctccc tatttcggca ttttttgcaa attcttgttg aaaaccactt tcattattaa
     1441 aatttcctac ttaaatttgt ggcataaata ccaaaacaat ggaaaacaac gaaaaacaaa
     1501 agtacgagat caaattgaac aaaaaatggt tcttagccgg acttgcagct actgcggtgt
     1561 gcatttggat ggtgattttt atgagtcagt ttttctggat tgcacttcca ttcgtgttta
     1621 ctttcctcgc tttgtctatt gacgcgttat aataaacgac aataatatta caagggtggt
     1681 aacggtttgt tatcaccctt tttttatttt tagcctatga aaaaactata cctcctgatt
     1741 gtcctggctc tttgttcaaa catacttttg gctcagtcaa acccttcatg gtttacttac
     1801 gatggcaccc ctacagcagg acgtttcgat gatatttttt tcatcaatga ttctaccggc
     1861 tgggctgtta gctctgatgg tgaaatatac aaaactgtaa acgcgggcaa aaattgggta
     1921 ttaaaactgg gggaaagcac ttacttccgt tctgttgagt tttttgatga aaacgttggt
     1981 tttgcaggca gtttattcgg cgaattatac aaaaccgttg atggtggcga ctcttgggaa
     2041 gacattggcg atttgttgcc gcatgtattc accggaattt gcgggttgag tgttgcagat
     2101 gacacttcca tttatgcctg tggtatttgg agtggctgtg cctatatttt taaatggaca
     2161 gatcgaggtg aaacttggtc atac
//
LOCUS       DAORKW010000596         2180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3111_length_2180_cov_4.314353, whole genome shotgun sequence.
ACCESSION   DAORKW010000596 DAORKW010000000
VERSION     DAORKW010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3111_length_2180_cov_4.314353"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PLB46_15910"
     CDS             complement(<1..993)
                     /locus_tag="PLB46_15910"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF78064.1"
                     /translation="MKKIMLMAAILMGVMIAKAQVPPPPPPPPPAGPVIKIEENKITI
                     ISPSGDEYIINEDNIMKNGVVLDTDSEAFRLEMDAIGKQLEALKLEDLNLTINNEDYA
                     ELMADLDELDAEMEALDNSGIQIDTLPGDSTSITIGNWRLIVAEDGEDAEDVDVSFGK
                     IPEVEEIDDRDVDVFDTEWWLFDMGYNTFLNSDYKAQIDEPYTAMEDLHGWGSFDVNL
                     HMFRSRVNIAKGYLNFNYGLSFEWHHFRFDNNFSIMPDVDTFTINTETIEYEKNKFNT
                     THLTLPVLIGFETKPWDTEHSFRMQFGYSPGLMLSGKTKLKHTGQKAIEKDDLNL"
     gene            complement(1021..1905)
                     /locus_tag="PLB46_15915"
     CDS             complement(1021..1905)
                     /locus_tag="PLB46_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78065.1"
                     /translation="MKININNYEEWMIDYIEGNLSDAQVKEMQEFLTFHPELKAELDL
                     FNETKLQPDTSVVFEHKESLKKAVGGRVVTMYGWVKYAASVAAVVMVFAGVRWFNSNN
                     TTEDNVAIKQYQYQQTDNNFAFDRTTDSIEQIPVDNTTPKEEKNYFADNKKKNVIEAI
                     PQEEIKEKIKRELNPIQSIDFAKANKIKSENNIEINANPIELLEENTFANNTIATPKT
                     TNSQITNISLNDNKNVIDWWQDAVAIGGEVGEVIEGVKDYDLNPIEPFLKHDNTQNEV
                     VKTRNINILGFNYYSRKK"
     gene            complement(1902..2114)
                     /locus_tag="PLB46_15920"
     CDS             complement(1902..2114)
                     /locus_tag="PLB46_15920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRF78066.1"
                     /translation="MQVDVKEAIEKALQKLPDVQKNCVLLRDYEGYSYEEIGEMLHLS
                     ESQVKVYIFRARQSLKEYLISIHHLI"
BASE COUNT          573 a          433 c          350 g          824 t
ORIGIN      
        1 gaggtttaaa tcatcttttt caatggcttt ctggccagtg tgtttcagtt tagttttgcc
       61 gcttaacatt aagcccgggc tgtatccaaa ctgcatacgg aagctgtgtt cggtatccca
      121 tggtttagtt tcaaatccga ttaacacagg caatgttaaa tgggtagtat taaatttatt
      181 tttttcgtat tcaatagttt cggtgttgat ggtgaatgta tccacatcgg gcataataga
      241 aaagttattg tcgaagcgga aatggtgcca ttcaaaactc aatccataat taaaattgag
      301 gtaaccttta gcaatgttta ctcttgagcg gaacatatgc aaattcacat caaaacttcc
      361 ccatccgtgt aaatcttcca tcgcggtgta tggctcatca atttgtgctt tataatcgct
      421 gttcaggaat gtattatatc ccatatcgaa taaccaccat tcagtgtcaa atacatctac
      481 atcgcggtca tcaatttctt ctacttccgg tattttgcca aaagaaacat caacatcttc
      541 tgcatcttcg ccatcttcag caacaattaa tctccaattg ccgattgtaa tagatgttga
      601 gtcgcctggt aaggtatcaa tctgaatacc tgaattatcc aaagcttcca tctctgcatc
      661 taactcatcc aaatcagcca ttaattcagc ataatcttcg ttgttgatag tcagatttaa
      721 atcttcgagt ttaagtgctt ctaattgttt accaatggca tccatttcta agcggaatgc
      781 ttctgagtcg gtatctaaaa caactccatt tttcataata ttatcttcat tgataatata
      841 ttcatcgcct gatggagaaa ttatagtgat tttattttct tcaattttaa ttactggccc
      901 agccggaggt ggtggaggtg gcggtggcgg cacttgtgct ttggcaatca ttactcccat
      961 caaaattgcg gccattaaca tgattttttt catagcgttg tatttaattg tataaataat
     1021 ttattttttt ctactgtaat aattaaaacc taatatgttg atgtttctgg tttttactac
     1081 ttcattttgt gtattgtcgt gtttcaaaaa aggttcaatc ggatttaagt cataatcttt
     1141 caccccttca ataacctcgc caacttcacc ccctatggct actgcatcct gccaccagtc
     1201 aatcacattt ttattatcgt ttaacgagat attggttatt tgactattgg ttgtttttgg
     1261 tgttgcaatg gtattgttgg caaatgtatt ttcttctaat agttcaattg gatttgcatt
     1321 aatttcgatg ttattttccg atttaatttt atttgcttta gcgaaatcaa tactttgaat
     1381 tggattaagt tcgcgtttta ttttttcttt aatttcttcc tgcggaattg cttcgattac
     1441 atttttcttt ttattatcgg caaaataatt tttttcttct tttggagtag tattatctac
     1501 cggtatttgt tctatactat cagttgttct gtcgaaagca aaattgttgt cagtttgctg
     1561 atattgatat tgtttaatgg caacattatc ttcggttgtg ttattgctgt tgaaccatct
     1621 tacacctgca aacaccatca ccactgctgc aaccgaagca gcgtatttaa cccagccata
     1681 catggttaca acgcgaccac caacagcttt cttaaggctt tctttatgtt caaacaccac
     1741 cgaagtgtcg ggttgtaatt tagtttcgtt gaacagatct aattccgctt tcagttcagg
     1801 atgaaaagtc aaaaactctt gcatctcttt tacctgagca tcagataaat taccttcaat
     1861 gtaatctatc atccattctt cgtaattatt gatatttatt ttcatattaa atgatgtata
     1921 cttattaaat attctttgag gctttgacgc gctctgaaaa tgtaaacttt aacctggctt
     1981 tcgctcaaat ggagcatctc tcctatttct tcgtaactgt atccctcata atctctcaac
     2041 aacacacagt ttttttgcac atcgggtaat ttttgaagtg ctttttcaat tgcttctttc
     2101 acgtccacct gcacttcagc tgttcaccct ttggctgctt catcaatttc ttccacgtaa
     2161 tccatccgtt tgtgtttgcg
//
LOCUS       DAORKW010000597         2180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3113_length_2180_cov_3.354353, whole genome shotgun sequence.
ACCESSION   DAORKW010000597 DAORKW010000000
VERSION     DAORKW010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3113_length_2180_cov_3.354353"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..690)
                     /locus_tag="PLB46_15925"
     CDS             complement(4..690)
                     /locus_tag="PLB46_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78067.1"
                     /translation="MKKLLLLFCICCSLSVFSQIEDYAGIGYAPRFVITSPIMDMLHA
                     YENGFDTVIIPFKEVKIIHGGSLYGSYGFNKFNMGFGLTISYAGYYGEAKLLSEPKSI
                     TDLSLYYFSFVYDVNYLVLNVEEFHAGPGVGFDLQTELISGKLTNTAGYSSFSGEVTS
                     NINPGFYFYVLLLTDLGDYTISLRPTYYYMLKNDTYDGLYDALIVDDVDNTFPGQTTY
                     FGLDLYLNRW"
     gene            753..977
                     /locus_tag="PLB46_15930"
     CDS             753..977
                     /locus_tag="PLB46_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HRF78068.1"
                     /translation="MEKLTYTNGEVTIIWQPKLCSHSGNCVRGLPQVFNSKIRPWINP
                     QGADTTQIIEQVKKCPSGALTFVMNADLNK"
     gene            1026..2138
                     /locus_tag="PLB46_15935"
     CDS             1026..2138
                     /locus_tag="PLB46_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRF78069.1"
                     /translation="MYEQVRLLKTITLINGYDGSISLQQYLKNWFKENKNAGSRDRKI
                     YTALVYNYFRIKGAATNYNQQALVVAAAAIDEITQPLHEAWKVETPEWQTAITNEIKF
                     PLEDRVAAAVDKEALFAALKKQPAVFIRVKQAMLGEVKQECINKGYEFTIDGHTMRFV
                     KNYPLTDMKTFTNSDFEIQDIASQQTIQFIQPNKNEAWWDCCAGSGGKSLMLLAHQPQ
                     VQLFVADIRKSILDNLKERFNRNGIINYSALIVDLTKSETFQRLPLFDGIIADVPCSG
                     SGTWTRTPEWLSFFDEQLLLESVSRQRTIVENICQQLKPGGKLVYLTCSVFSDENENN
                     VAYFTQHLPLQLVEQKYFQYSSEGGDTLFGAVLKKN"
BASE COUNT          773 a          382 c          405 g          620 t
ORIGIN      
        1 agttcaccac ctatttaagt ataaatccaa accaaagtag gtagtttggc cagggaatgt
       61 attatcgacg tcgtcaacaa ttaatgcatc atataaacca tcgtaagtat catttttgag
      121 catataataa taggttggtc ttaacgaaat cgtatagtcg cctaaatctg tcagtaataa
      181 cacataaaaa taaaaaccgg gatttatatt acttgtaacc tcgcctgaaa aactactata
      241 tccagcggtg ttggttaact taccagaaat cagctctgtt tgtaaatcaa aacctacacc
      301 cggcccggca tgaaattctt cgacattgag aactaaataa ttaacatcat atacaaatga
      361 aaaataatat aaggataaat cggttataga ttttggttcc gataatagtt tagcttcccc
      421 ataataccct gcataagata ttgttagtcc aaaacccatg ttaaatttat taaacccgta
      481 cgagccgtat aacgatccac catgaatgat tttcacttct ttaaatggaa ttattacagt
      541 atcgaaccca ttttcgtaag catgcagcat atccataata gggctagtga tcacaaaacg
      601 aggggcgtaa ccaataccag cataatcttc aatttggctg aataccgata acgaacaaca
      661 aatacaaaat aggagtaata gttttttcat aaaaacatat aatgggtata aaattacttt
      721 ttttaaatag atttgtataa acaggcaaaa acatggaaaa gttaacttat accaacggcg
      781 aagtgactat tatttggcaa ccaaaacttt gcagccattc aggcaattgt gtcagagggt
      841 tgccacaggt atttaacagt aaaatacgac cctggataaa tccgcagggc gctgacacaa
      901 cccaaattat tgaacaagtg aaaaaatgcc cttcaggtgc attgactttt gtaatgaatg
      961 ccgacctgaa taaataggtt ttgggaactt tcagatgcta aatgtaaaac gattactttt
     1021 acaagatgta cgaacaggtc cgattactta aaaccattac attgattaat ggttacgatg
     1081 gtagtatatc attacaacaa tatttaaaaa actggttcaa agaaaataaa aatgccggga
     1141 gtcgcgacag gaaaatttac acggcgttgg tatataatta tttcagaata aaaggcgctg
     1201 cgaccaatta taatcaacag gctttagtgg ttgcggctgc agcaatagat gaaatcacac
     1261 aacctctgca cgaggcatgg aaagtggaaa cacccgaatg gcaaactgct ataaccaatg
     1321 aaataaaatt cccgcttgag gaccgtgttg cagcagctgt cgataaggaa gcattatttg
     1381 ctgcattaaa aaaacaacct gcagttttta ttcgtgtaaa acaagcaatg ttgggtgaag
     1441 taaaacaaga atgtattaac aaaggatacg aatttactat tgatggccac acgatgcgat
     1501 ttgtaaaaaa ttatccatta acggatatga aaacatttac aaatagcgat tttgaaatac
     1561 aggatattgc ttcacaacaa accatacaat ttattcagcc caacaaaaat gaagcctggt
     1621 gggattgttg tgcaggttca ggagggaaat cactgatgtt actggcacat cagccacaag
     1681 tgcaattgtt tgttgccgac atccgaaaaa gtattttgga taacctgaag gaaaggttta
     1741 atagaaatgg tatcatcaat tattcggcac ttattgtaga tttgacaaaa agtgaaacat
     1801 ttcagcgatt accgttattt gacggcatta ttgcagacgt tccatgcagt ggttcaggca
     1861 cctggaccag aacaccggaa tggttatcgt tttttgatga acaattactt ttggagtctg
     1921 taagcagaca acgcaccatt gttgaaaata tttgtcagca acttaaaccc ggtggtaaat
     1981 tagtgtattt aacctgttcg gtgtttagcg atgaaaatga aaacaatgtg gcttatttta
     2041 cacaacatct gcctttacaa ttggttgagc aaaaatattt ccaatattct tcggaaggag
     2101 gtgatacact ttttggcgct gttttgaaaa agaactaagc cgttttattg aaactataag
     2161 tgatagctcg tgaactattt
//
LOCUS       DAORKW010000598         2176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3117_length_2176_cov_7.457803, whole genome shotgun sequence.
ACCESSION   DAORKW010000598 DAORKW010000000
VERSION     DAORKW010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3117_length_2176_cov_7.457803"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..>2176)
                     /locus_tag="PLB46_15940"
     CDS             complement(199..>2176)
                     /locus_tag="PLB46_15940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78070.1"
                     /translation="DYAIGASWVAATTGISAGNHPFFVDMAENNIDIAYVAGANSSTS
                     YPIVFKTINAGTNWSAVLLTNNNENIYTGYQGDNGDEGWYYDEFAEGFDVSATNPNVA
                     IISGLGYPHFTNDGGVTWKQQYVNPADQNPLNADIVKGKNYAGVGLENTSCWSLCWGD
                     ANNIIAGFSDITAIRSVDAGAKWNKGLTGITENSVYHVIKDENSGKIYCGTSSAHDMY
                     QSTYLKDDVINGATGRILSSTDNGANWTLIHDFADPVIRLALDPNTANKMYAAVIHST
                     NGGIYVSSNINLGASSTWTKLSNPPRTEGHPYDIKVLNDGTLIAVYSGRRTAAGVFTA
                     SSGVFKSTDGGTTWTDISDPGMFYWTKDLIVDANDASQNTFYCAVFSGWGGAPNGLGG
                     IYRTTNRGTSWTKINALDRVESVTIDPNDNNIMYATTESEGLWITHNLNTASPVFIQD
                     LTYPFQHPVRVIFNPLNSNEVWITSFGNGLKVGYLDNCIAPTDLVASDITPTSATVTW
                     DAVPGADSYRITRKQVGGLAYNYNSVSNTFTMTDLIPGTVNKIFVKSKCDGVFSDKSD
                     KITIYVPAKFGEIENALHIFPNPTHNYFEISYRSNERGEAVISLSDISGRIIFSEQYD
                     LNIGENNFSYDVSHLQKGVYILSFGENRSKIIID"
BASE COUNT          659 a          444 c          358 g          715 t
ORIGIN      
        1 aactttaaac actattcgaa aaacacctct gtatattctt tgtgggctgt ttctttgtgg
       61 actctgtggt aaaaaaatgt gctgatgtgt taatgtgcag atttaacagc cgaaaccacg
      121 gagaaacaca aagcgaactc acacagaggg agcccagagt ttgtattttc tttctggtta
      181 cgactgttat agcatttatt aatctataat tattttactt ctgttttcgc cgaagctaag
      241 gatatataca cctttttgca gatgacttac atcgtaacta aaattatttt ctccgatatt
      301 taaatcatat tgttccgaga aaataattct tcctgatata tcacttaaac ttattactgc
      361 ttcacctctt tcgttactac gataacttat ttcgaaataa ttatgtgtag gatttgggaa
      421 aatgtgtaat gcgttttcta tttccccaaa tttagcagga acataaatgg ttattttatc
      481 tgatttatca gaaaacacac catcacattt cgattttaca aatattttat ttaccgttcc
      541 gggaattaaa tctgtcatag taaaagtatt actaacgcta ttataattat acgctaaacc
      601 tcccacctgt ttgcgtgtaa tgcgataact atccgcaccc ggaacagcat cccaggtaac
      661 agttgcagag gttggcgtga tatcactggc cactaaatcg gtgggtgcga tacaattatc
      721 caaatagccc acttttaaac cattgccaaa gctggtaatc caaacctcat tggaatttaa
      781 aggattaaat attacccgaa caggatgttg aaacggataa gtaaggtctt gtataaatac
      841 gggacttgct gtgtttaaat tatgtgtaat ccaaaggcct tctgattcgg ttgtggcata
      901 cataatatta ttatcgtttg gatcaatggt aacagattcg acacgatcca aagcatttat
      961 ttttgtccat gaagtaccgc gattagttgt tctgtaaatt ccacctaacc catttggtgc
     1021 tccaccccat cctgaaaata ccgcacaata aaatgtattt tgagaagcgt cattagcatc
     1081 cacaattaaa tcttttgtcc aataaaacat tcccggatct gaaatatctg tccaggttgt
     1141 tccaccatct gtagatttaa acacaccact actcgcagta aatacacctg ctgcagttct
     1201 tcggcctgaa taaactgcga ttaatgttcc atcgtttaat actttaatat catatggatg
     1261 tccttcagtg cggggaggat tggaaagttt agtccaagtg cttgaagcgc ctaaattaat
     1321 attggatgaa acataaatgc ctccattagt ggaatgaata actgccgcat acattttatt
     1381 tgcagtatta gggtctaacg ccaatcgaat tacagggtct gcaaaatcgt gaattaaagt
     1441 ccaatttgca ccattatcag ttgagcttaa aattcttccg gttgctccat taattacatc
     1501 atcttttaaa tatgtacttt gatacatatc atgtgcgctt gatgtgccac agtatatttt
     1561 tccagaattt tcatctttga taacatggta aacagaattt tcagttattc ccgttaatcc
     1621 tttattccat tttgcacctg catctacact tctaattgcg gtgatatcac taaaacctgc
     1681 aatgatatta ttcgcatcac cccaacacaa actccagcag gaagtatttt ctaaacctac
     1741 accggcataa ttttttcctt tgacaatatc tgcatttagt ggattttggt cggcggggtt
     1801 tacatattgt tgtttccagg taactccacc atcattggta aaatgcggat aacccaaacc
     1861 cgagattata gctacattcg ggttagttgc tgaaacatca aatccttcgg caaattcatc
     1921 gtagtaccat ccttcatcac cattatcgcc ttgatatccg gtataaatat tttcattatt
     1981 attcgttaat aaaactgcag accaatttgt acccgcatta attgttttaa atacaattgg
     2041 ataagaagtt gatgaattgg ctcctgcaac atacgcaata tcaatattgt tttcggccat
     2101 gtccacaaaa aacggatgat taccggcact tatgccagtt gttgcagcaa cccatgacgc
     2161 acctatcgca taatcc
//
LOCUS       DAORKW010000599         2173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3124_length_2173_cov_3.586874, whole genome shotgun sequence.
ACCESSION   DAORKW010000599 DAORKW010000000
VERSION     DAORKW010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3124_length_2173_cov_3.586874"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PLB46_15945"
     CDS             complement(<1..429)
                     /locus_tag="PLB46_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C1A papain"
                     /protein_id="HRF78071.1"
                     /translation="MAIRMVQDENQPQQQRKLPGNGGGNRGGGGGGNIITMLLPLLLG
                     LFRKNPKMGCGVLIVGAVILYFFGGSLFNGGGGGATNITDLFNTGASFDAAKYDATEI
                     FEPPITDNAKNPLPEAISLLEYAPDRKNQGSQGSCVGWGGA"
     gene            542..1168
                     /gene="rsmG"
                     /locus_tag="PLB46_15950"
     CDS             542..1168
                     /gene="rsmG"
                     /locus_tag="PLB46_15950"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRF78072.1"
                     /translation="MDRILKYFPNLDNKQISQFRQVGLQYRFWNEKVNVISRQDIDNI
                     YEHHVLHSLAIAKFIDFQPGTTVLDLGTGGGFPGIPLAIMFPEVHFHLVDSIDKKVKV
                     VESIYKALGLRNVTVEHTRVEDLPDTYDFIVTRAVADMMILYDWCINRIKGGGSFNDF
                     PNGLIMLKGGFLDLEIAPFKEILYKFPITEWLPEQWFVDKYLLYLPMD"
     gene            complement(1207..>2173)
                     /locus_tag="PLB46_15955"
     CDS             complement(1207..>2173)
                     /locus_tag="PLB46_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78073.1"
                     /translation="FNFDLNYKTATDKFLPQSNYYLNTGVLDGVFAFETGIEKKMGRA
                     GAHKLSVYVKDLYFFNTMYLYNHELIEEAKNNSVNIDYEHNYNYFTGNGQLNVKFRSN
                     DLLSDYDFSRINIEAINKTKLGKFDLRTRLFGQWGSGSDMPFESSLMLAGANTEELLD
                     NKYTRSRGFFPDEWVDFGETTGHFHAGGGLNLRGYAGYLVADTSAEEGSINAFSGMSG
                     VAANVELQFNRLFKFQLIKFIKMEPYLFADAGIISTDDRFDRVADLRVDAGIGTAFTW
                     SWFGPLETVKPITLRVDFPLFLNRTPYEDGEFLQFRYVIGINRAF"
BASE COUNT          673 a          443 c          364 g          693 t
ORIGIN      
        1 ggcacctccc caccctacgc aactaccctg agaaccttgg tttttgcggt caggtgcata
       61 ttccaaaaga gaaattgctt cgggtaaagg gtttttagca ttatcggtaa ttggcggttc
      121 aaatatttcg gtagcatcat attttgctgc atcgaaactg gcgccggtat taaataaatc
      181 ggtgatgttg gttgcaccac ctccaccgcc attaaataaa ctgcctccaa aaaaatagag
      241 aataacagca ccaacaataa gcactccgca acccattttc gggtttttac ggaataatcc
      301 tagtaacaag ggtaataaca tggtaataat attaccacct cctccaccgc cacggtttcc
      361 accaccattt cccggaagtt tacgctgttg ttgaggttgg ttctcatctt gcaccattcg
      421 aatagccata agtcacaatt ttgcgtgaaa gttatcattt tttcgataat tcgggatgta
      481 atattttttg ttatccatgc gttattttga aaatcagcct gaaaatcact aaacttgttt
      541 cgtggaccgc attttaaagt atttccctaa tttagacaat aagcaaatca gccagttcag
      601 acaggttgga ttgcaatacc gtttttggaa tgaaaaggtg aatgtaattt ctcgtcagga
      661 catagataat atctatgaac accacgtttt acattcgctg gcaattgcca aatttattga
      721 cttccaacca ggcactaccg tgttggattt aggaaccggt ggcggatttc cgggtattcc
      781 tttagccatt atgtttcccg aggtgcattt tcatttagtt gattcgattg ataaaaaagt
      841 gaaagtagtt gagtcaattt acaaagcttt aggcttgcgt aatgtgactg ttgaacatac
      901 ccgtgtagag gacttgcccg atacatacga ttttattgta acaagggctg tagcagatat
      961 gatgattttg tatgattggt gtatcaatcg aataaaagga ggtggctcat ttaatgattt
     1021 cccaaacggt ttaatcatgt taaaaggtgg ttttttagat ttagaaattg caccctttaa
     1081 agaaattctg tataaatttc caattactga atggctaccc gagcaatggt ttgtagataa
     1141 atatttatta taccttccaa tggattaaaa aaaaggctat tgaaaaccat caatagcctt
     1201 ttttatttaa aatgctctgt taattccaat tacatatctg aattgtaaaa attcaccgtc
     1261 ctcataaggt gtgcgattta aaaatagtgg aaaatcaacc cttaaggtaa taggttttac
     1321 cgtttcaagc ggaccaaacc aactccaggt aaatgcagtt ccaataccgg catctactcg
     1381 caaatctgca acgcggtcaa atctatcatc agtgcttatt attcctgcat ccgcaaataa
     1441 ataaggttcc attttaataa atttaattaa ttggaattta aataatcggt taaattgtaa
     1501 ttccacgttt gctgcaacac cactcatacc gctgaatgca ttaatactgc cttcttctgc
     1561 tgaagtatct gcaaccagat aaccggcata accacgcaag tttaatccac caccggcatg
     1621 aaaatgtcct gttgtttcac caaaatcaac ccactcatcc gggaaaaatc cacgtgacct
     1681 tgtgtattta ttatccagca attcttctgt gtttgcacct gccaacatca gcgatgattc
     1741 aaaaggcata tcagaaccac taccccactg cccgaataat ctggttcgca aatcaaactt
     1801 gcctaatttc gttttattaa tggcttcaat atttattcgt gaaaaatcat aatcgctcaa
     1861 caagtcgtta ctgcgaaact tcacatttaa ttgaccattc cctgtaaaat aattgtaatt
     1921 gtgctcgtaa tcaatattta ccgaattgtt ttttgcttct tcaattaact catgattgta
     1981 caaatacatg gtattaaaga aatataaatc ttttacataa acagataact tatgtgctcc
     2041 cgctcttccc atctttttct caatacctgt ttcaaatgca aaaacaccat ctaacacacc
     2101 tgtatttaaa taataattac tttgcggaag gaatttatcg gtagctgttt tgtagttcaa
     2161 atcaaaattg aac
//
LOCUS       DAORKW010000600         2168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3144_length_2168_cov_4.053478, whole genome shotgun sequence.
ACCESSION   DAORKW010000600 DAORKW010000000
VERSION     DAORKW010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3144_length_2168_cov_4.053478"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLB46_15960"
     CDS             <1..613
                     /locus_tag="PLB46_15960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78074.1"
                     /translation="GTAAAPIFTIQSEFWGNVDVRAPLQVFGSSAPFLFINQDGEKQL
                     YVGCEDGTIYLYTPTPDFTGAFTEVTSNFSGIDDGNYSALSIADIDNDSYPDFVTGNL
                     RGGITFYRNSLSTPIFNAPIVDNQVQLSPNPVTSNLNITALDNWLFNKVAVFSLDGKL
                     WQTNSFGLTDNYQISLSELPAGFYIVTIYDNAGNNLVKTFIKH"
     gene            617..>2168
                     /locus_tag="PLB46_15965"
     CDS             617..>2168
                     /locus_tag="PLB46_15965"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF78075.1"
                     /translation="MKKVLLLFSMLFWLNAKAQYIPNTTITAAQYGSELNLPFIGGLT
                     APQFSDVDVNFDGINDLFIFDRGTWLPIVLIKDEDGNFSYHPEYANNFPVIEDWVLLR
                     DFNCDGIMDIFTYYIGSTRVYKGIEIAGEINFTLEKSMLEYADFTGTTALYTSRTDIP
                     AIDDIDSDGDLDILSFSVSNSTIRFYENVSVESGFGCDSLIFELNELCWGSLYEGFTC
                     YGGDLHVACKGGDATEEETENTRAHIGSTIVTYDQDGDGDVDAILGDNSCNNLVYYRN
                     GGSVSSAEMDKKDSLWQSNGKEYDMPVFPAGYFVDYDNDNDDDLIVTTNDHQLGLNTE
                     HIWLYENFNTNDTFDLQFQTDTFLISEMIDIGSYSKPIFFNYNNDNLPDIVAGVSTTF
                     GKSATFKYGLWLYKNTGTTTLPKFELVSKDFGGLNAYAQSHLAPASGDIDNDGDDDLL
                     VGNFDGNIIFLENLSDGIGNAVMATPQVLYQTIDVGSNATPCLIDIEQDGKLDLVVGE
                     ANGNLNYYH"
BASE COUNT          677 a          328 c          438 g          725 t
ORIGIN      
        1 aggaacagcc gctgcgccga tttttaccat acaaagtgaa ttttggggaa atgttgatgt
       61 tagagctcct ttgcaggtgt ttggttcatc tgcacctttt ttgttcatca atcaagacgg
      121 tgaaaaacag ttatatgtag gctgtgaaga tggcacaatt tatttgtaca cacctacacc
      181 cgattttaca ggtgcattta ctgaagttac ttcaaatttt agtggtattg atgatggtaa
      241 ttacagcgct ctgagcattg cagatatcga taatgatagt tatcctgatt ttgtaacagg
      301 aaatttacgc ggcggaataa cattttatcg caattcactt tcaacgccaa tatttaatgc
      361 ccctattgta gataatcaag tacagctttc accaaatccg gtaacatcaa atttaaatat
      421 cactgcgttg gacaattggt tattcaacaa agtagcggtg tttagtcttg atggtaaact
      481 ttggcaaaca aatagttttg gtttaaccga caactatcaa atttccttat ctgaattacc
      541 agccgggttt tatatcgtta ccatttatga taatgccgga aacaatctcg taaaaacctt
      601 tattaaacat tgagatatga aaaaggtatt attactattt tcgatgttat tttggttgaa
      661 cgcaaaagct caatatattc ctaatacaac aattacagct gctcaatatg gaagtgaatt
      721 gaatttacct tttataggtg gtttaactgc gccgcagttt tcagatgtag atgtaaattt
      781 tgacggtata aacgacttat ttattttcga cagaggaaca tggttgccga ttgttttaat
      841 aaaagatgaa gatggtaatt ttagttatca tccggaatat gccaataatt ttcctgttat
      901 agaagattgg gttttattac gcgattttaa ttgcgatggc atcatggata ttttcactta
      961 ctatattggt tcaacaaggg tttataaggg tatagaaatt gccggtgaaa ttaattttac
     1021 cttagaaaaa tccatgcttg aatatgccga ttttaccggg acaacagcat tgtataccag
     1081 cagaacagat attcccgcaa ttgacgatat tgatagtgac ggtgatcttg atatattatc
     1141 gttttctgtt tctaattcaa ccatccgatt ttatgaaaat gtttcagttg aatcgggatt
     1201 tggatgtgat agtttaattt tcgaattaaa tgagttgtgc tggggaagtt tatatgaagg
     1261 gtttacctgt tatggtggcg atttacatgt tgcctgtaaa ggtggagacg ctacagaaga
     1321 agaaacagaa aatacacgcg ctcatattgg ctctacaatt gtcacttacg atcaggatgg
     1381 agatggcgat gtagatgcta ttttaggtga taattcctgc aataatctgg tgtattacag
     1441 aaatggtggt tcagtgagtt ctgctgaaat ggataaaaaa gatagtttgt ggcagtctaa
     1501 cggtaaggaa tatgatatgc ctgtttttcc ggcaggttat ttcgtggatt atgacaacga
     1561 taacgatgac gatttaattg taacaaccaa cgaccaccag cttggattaa atacagaaca
     1621 catatggtta tacgaaaatt ttaataccaa cgatactttc gatttacaat ttcaaaccga
     1681 tactttttta atcagtgaaa tgattgatat tggtagttac tcaaaaccta tcttttttaa
     1741 ttataacaat gataatttac ccgatatagt tgcaggcgtg agcactacgt ttggcaaatc
     1801 ggcaacattt aaatatggat tgtggcttta taaaaataca ggcacaacca cattaccaaa
     1861 atttgagttg gtgagtaaag attttggtgg attaaatgca tatgcacaat cgcatttagc
     1921 acctgcgagt ggtgatatag ataacgatgg tgatgatgat ttattggttg gtaattttga
     1981 tggcaatatt atttttcttg aaaatttatc cgacggaatt ggtaatgcgg ttatggcaac
     2041 tccgcaagtg ctgtatcaaa ccattgatgt gggcagcaat gccacgccat gtttaattga
     2101 cattgaacaa gatggtaagt tagatttagt agttggtgaa gctaatggca atttaaatta
     2161 ttaccata
//
LOCUS       DAORKW010000601         2153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3180_length_2153_cov_4.382745, whole genome shotgun sequence.
ACCESSION   DAORKW010000601 DAORKW010000000
VERSION     DAORKW010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3180_length_2153_cov_4.382745"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="PLB46_15970"
     CDS             complement(<1..1608)
                     /locus_tag="PLB46_15970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRF78076.1"
                     /translation="MKKQVLSILILIFTACITNAQTDASLAQQFMSNGEYDKAADIYK
                     KLFNENPNSNYKPYYNCLMLLNDFKEIERIAEKQMKKNPDNLTYYVDLGNALIKQGNK
                     KEAEKQFDIAIQKISDNKQQVIQLANAFIGLDNLERAITVYEKGQKIVTTHTFNYDLA
                     GLYYRLGNLDLSLDTYLTYYGENDNNGTSKTTSALTRILDEESDHQLLQEKLFQRIQK
                     GNNEILYTELLIWDYIQLKDFEGAFIQTKALDKRNKENGERVYELAETARLESEYDAA
                     IDGFNYIISKGDSYPYYFSSKNGILNCRKEKIFKTNSYTIEDINQLKASYQEFLNEYA
                     RKDIRAANVTNDLSKLEAFYIHDVANAITLLEPIVEWPSLNLTDKSRLKLDLGDYYLI
                     SGDVWEATLLYSQVDKAMKDAPLGEEARYKNAKLAYYRGDFNFAQTQLNVLKAATSEL
                     VSNDAMKLSVFITSNLGLDSVVEPMMLFAEADLFVFQNKLTDAVLTLDTLDKTFPAHQ
                     LADDILYLKADIALKKQNLNEAVKLLEE"
     gene            complement(1676..>2153)
                     /locus_tag="PLB46_15975"
     CDS             complement(1676..>2153)
                     /locus_tag="PLB46_15975"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HRF78077.1"
                     /translation="VATEELAELVVQEDDKVIEKTESTESTPSDSTTTGTKTDGNNPN
                     SNVTGNGKTVYLSTEVKPEYPGGTKGLNRYLSDNLKYPDVARDNGITGIVTVSFIINE
                     DGSVSNAKVERSIGGGCDQEALRIVSEMRKWKPAKQGGVPVAVYMKLPIVFQLQDN"
BASE COUNT          603 a          393 c          372 g          785 t
ORIGIN      
        1 ttcttctaac aatttaacag cttcgttgag gttttgtttt ttgagggcga tatcagcttt
       61 taaatataat atgtcatctg ccaattgatg tgcaggaaat gttttgtcga gtgtatccaa
      121 agttaacact gcatcagtta atttattttg gaaaacaaat aagtcagctt cggcaaacaa
      181 catcatgggt tcaactacag agtcgagtcc gagatttgat gttataaata ctgataactt
      241 catagcatca tttgaaacta attcgctggt tgccgctttt aaaacattaa gttgtgtttg
      301 cgcaaaatta aaatcgcctc gataataggc tagtttcgca tttttataac gtgcctcctc
      361 accaagcggt gcgtctttca ttgctttgtc tacttgcgaa tataataatg tagcttccca
      421 aacatcacca ctaattaaat agtaatcgcc caggtcaagt tttaaacgcg acttatccgt
      481 taaattcaat gatggccatt ctacaattgg ttctagtaat gtaatagcat ttgcaacatc
      541 gtgtatataa aaagcctcca gtttagataa atcgttggtg acatttgcgg cgcgaatatc
      601 ttttcgtgcg tattcgttta aaaactcctg atagctggct tttagctgat tgatatcttc
      661 aatagtataa ctattggttt taaatatttt ttctttgcga caattcaaaa tgccattttt
      721 cgaactgaaa taatacgggt aactatcacc tttggaaata atgtaattaa atccatcaat
      781 agctgcgtcg tattcacttt caagtcgagc ggtttcggct aattcataaa cgcgttcacc
      841 gttttcttta ttccgtttat ccagtgcttt tgtttgaata aaagcacctt caaagtcttt
      901 taattgtata taatcccaaa ttaacaattc ggtatataaa atttcattat ttcctttttg
      961 tatccgttga aataattttt cctgtaacaa ttggtgatca ctttcttcat ctaaaattct
     1021 tgttaaagca gaagtagttt ttgacgtacc gttattgtca ttttcaccgt aataagttaa
     1081 ataagtatct aagctcaaat ccagattacc caatcggtaa tataaccctg caaggtcgta
     1141 attaaatgta tgcgtggtca caattttttg tcctttttcg tacacggtta ttgctcgttc
     1201 caaattatca aggccaataa atgcattggc gagttggatt acttgttgtt tgttgtctga
     1261 gattttttga attgcgatat caaattgttt ttcagcttct tttttattgc cttgttttat
     1321 caaagcattt cctaaatcaa cataataagt gaggttatca ggattttttt tcatttgttt
     1381 ttctgcaatt cgttcaattt ctttaaaatc gttgagcagc attaaacaat tataatatgg
     1441 cttatagtta gaattcggat tttcgttaaa taatttttta tagatgtcgg cagctttatc
     1501 atattcacca ttactcataa attgctgagc aagcgatgca tctgtttgcg catttgtgat
     1561 gcaggcagta aaaattagta ttaatatgct gaggacttgt tttttcatac gagttataca
     1621 acgaaaataa tgccgaaagg attgtaaatg ttcattttaa acacaaatta actaattaat
     1681 tatcctgtag ttgaaaaacg ataggtaatt tcatgtagac agcaacggga acaccgcctt
     1741 gttttgccgg tttccatttg cgcatttctg aaacaattct cagtgcctcc tggtcgcaac
     1801 cgccgccaat actgcgttct actttggcat ttgaaacgga accgtcttca tttataataa
     1861 acgacacggt tacaattccg gtaatgccgt tatcacgtgc tacatccgga tatttaagat
     1921 tatcactgag gtaacggttt aagcctttgg ttcctcccgg gtattccggt ttaacttcgg
     1981 tgctcaggta aacagttttg ccattgccgg ttacattgga atttggattg ttgccatcgg
     2041 ttttagtgcc tgtcgtggta ctatcggaag gggtgctttc ggttgattca gttttttcta
     2101 tcactttatc atcttcctga acaaccaatt ccgccagttc ttcagtcgca act
//
LOCUS       DAORKW010000602         2150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3189_length_2150_cov_4.105012, whole genome shotgun sequence.
ACCESSION   DAORKW010000602 DAORKW010000000
VERSION     DAORKW010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3189_length_2150_cov_4.105012"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..1300)
                     /locus_tag="PLB46_15980"
     CDS             complement(185..1300)
                     /locus_tag="PLB46_15980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF78078.1"
                     /translation="MSALFIVAATLIYTDNLATKLAKEEKQKVAQIANVYHYIATATD
                     ITDYGFFVELIQANTTVPIISTDNEGHIVAHLNLDTAKVAADSTYLPRCLAAMKDYAE
                     PIKLEISSGIYQYVYYKHSILYQQLLYYPYVQLLIIAAFLIVAYTLFNTARKSEQNRV
                     WVGMAKETAHQLGTPISSLVAWVEYLEQSPEMEQKRSVVEEMGKDVQRLELIADRFSK
                     VGSQPDLTEKNLITELEYSIDYIKRRSSKKVSFDFQYPNPNMQVKMNVILFNWVIENL
                     LKNALDAIDGIGDIVIRVEEDTDHAIIDISDSGKGIPRSKQKTVFEPGYSTKKRGWGL
                     GLTLAKRITESYHKGKIFVKQSAPGKGTTFRIILPKS"
     gene            complement(1439..>2150)
                     /locus_tag="PLB46_15985"
     CDS             complement(1439..>2150)
                     /locus_tag="PLB46_15985"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRF78079.1"
                     /translation="TNIDVAQLFEQFDNFDQTTLTEKNIKGRISANLYEMNIRWDKDF
                     KLDEQSIYTLCDMKIENGELIDYKPLESLSGFVKMKELRHIKFSTLENKIEIKDRVII
                     LPAMQIKSSALDMSISGKHTFDDVIDYQFKMSLADMMVRKFLGGNKQQDNYEEDAEGG
                     VNVYISMTGTVNDPIIEYNKREAKQKLKESGLEEQRFIDIFKRDPEEEMFKTNTVPEK
                     QSTTPADEIEFIEYEDEL"
BASE COUNT          641 a          441 c          327 g          741 t
ORIGIN      
        1 cggctgtttc tacctcggct taggcttcac gaatccgcac gtaaataaac tggattaatt
       61 tttaaatttt gtgccttttg cgcaattaat tttgcgttcc ggttcaccgg aattgcgggc
      121 taaaaaaatg ttatttcgaa acctacaata acaaataaac acccaacaat taaaataaaa
      181 tcacttaaga ttttggaaga ataattctga aggtagtccc tttacccgga gcactttgtt
      241 tgacaaatat tttcccctta tgataacttt cggtaatacg ttttgcaagc gtaagtccca
      301 aaccccagcc gcgttttttc gtgctatatc ccggctcaaa caccgttttt tgttttgacc
      361 ttggtatacc tttcccgctg tcgctaatgt cgataatagc atggtcggta tcctcctcaa
      421 ccctaattac aatatcgcca ataccatcaa ttgcatccaa tgcatttttc aataaatttt
      481 cgatcaccca attaaataaa atcacattca ttttaacctg catattcgga ttgggatatt
      541 ggaaatcaaa actcactttt ttagacgagc ggcgcttgat ataatcaata gaatattcaa
      601 gttcggtaat cagatttttc tccgttaaat caggttgaga cccaactttt gaaaatctgt
      661 ccgcaattaa ttccaaccgc tgtacatctt tccccatttc ttctaccaca cttcgttttt
      721 gttccatttc aggtgactgc tctaaatatt ccacccaggc taccaacgaa gaaataggtg
      781 tgcctaattg atgtgcagtt tctttcgcca ttcccaccca aacacggttt tgttccgatt
      841 tgcgtgcggt attaaataag gtatacgcca caattaaaaa tgccgctatt attaataatt
      901 gcacataagg ataatataat aactgctggt ataaaataga atgtttataa tacacatact
      961 gataaatccc gctactgatt tctaatttaa tgggttcagc ataatctttc atagctgcta
     1021 aacacctggg caaataagtt gaatcggctg ccacttttgc tgtatctaaa tttaaatgcg
     1081 caacaatatg tccctcattg tcggttgaga taataggaac cgtagtattt gcctgaatga
     1141 gttcaacaaa aaaaccgtaa tcggtaatat cagtagctgt tgcaatgtaa tgatacacat
     1201 tggcaatttg agcaaccttc tgcttttctt cttttgcaag tttagtagcc agattgtcgg
     1261 tataaattaa agtagccgca acaataaaca gtgccgacaa gactaaaact attttccaac
     1321 ggtattttct ttcgtaaata ttcaacaggt tatccggtta attgtcaaca aaaatggtaa
     1381 aaaaggagga caaacccaag cagccacttt tattggtatt tgcgttatag ggttaatctt
     1441 aaagctcatc ttcatattct ataaactcaa tttcatcagc cggagtagtg ctctgttttt
     1501 caggaactgt atttgtttta aacatttcct cttcggggtc gcgtttaaat atatctataa
     1561 aacgttgctc ttctaatccg ctttctttta atttttgttt cgcctctcgt ttattgtatt
     1621 caataatcgg gtcgttaacc gtgcctgtca tactgatata tacatttaca cctccttctg
     1681 catcttcttc ataattgtcc tgctgtttgt tgccgcctaa aaatttacgg accatcatat
     1741 ctgctaaact catcttaaac tgataatcaa ttacatcatc aaatgtatgt ttaccactaa
     1801 tagacatatc cagcgcggaa gatttaattt gcatggcagg taaaatgatt acccggtctt
     1861 taatttctat tttattttct aaggtcgaga atttaatatg acgcaattct ttcatcttca
     1921 caaatcctga aagtgactct aatggtttgt aatcaattaa ttcaccattt tcaattttca
     1981 tgtcgcataa ggtataaata gattgttcat ccaatttaaa atctttatcc caacgaatat
     2041 tcatttcata taagttagcc gaaattcttc cttttatatt tttttccgtg agcgtagtct
     2101 gatcgaaatt atcaaattgt tcaaacaatt gtgcaacatc aatattggtc
//
LOCUS       DAORKW010000603         2148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3194_length_2148_cov_4.102246, whole genome shotgun sequence.
ACCESSION   DAORKW010000603 DAORKW010000000
VERSION     DAORKW010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3194_length_2148_cov_4.102246"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2148
                     /locus_tag="PLB46_15990"
     CDS             <1..>2148
                     /locus_tag="PLB46_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78080.1"
                     /translation="FRSAKSSDANYVYQVYPVITNDGVYFISLEGVFRTSIYEQKGGM
                     TMRSGSTELRLSLHDLNKGTLIKREVIGDYHKMYSSIIGARDSIFWMYNTTDGLHGRS
                     VKALETIITQEQLIASNPALSEGLAMSQSTLTNLSELYAFDREHNALMLTTISGKKIW
                     VDGASFKTIESPIAYLPKNSYADIIERTIEQALAGETIDVNLITEQILQATAGVNTRF
                     ELDHLSDRIVAFDSCTYKLEGGTLRAITKTDCPKPILRNTNSAGDVKFIEPHLISTYN
                     SEKKLAVNPEFLPGNMSIITHAKSIGDKTELLISGINNTNLQKQFTIATGITLSNYRS
                     GYKISAVYGSQDTLLLGINNQLFNINTKTGKLIWKKVIAKEDYNAQLLSVGEAVQNGK
                     RYIVVVNNYFTVLSKQGIFVNGRTDYQQLVIDAASGKILHQQDINNSQPEKLPYYLGN
                     AHGLNWFYNDEIGFHTRSFPKLIPEQNDFTSSLKVAEITSPLVKTSGYGRAIDDKYIS
                     FDAAKKLCYFTTENGLHYVYDITNKKISEINAPDDALYHNLKMQNLNVNYYYRNAYYS
                     TPDRIELPNGCALTAKNENAIPVLRYEAAQKSSPTASTTTSTQFIEGEFLINGISLNK
                     DIFYTDKNYNPISTNNKESFCYIWHKNKIAVDAHRIISKYNYTTQQSAWQFDVTALLG
                     VEGEISRVYNRKSSLVIIFKTHPKLDDNFTCVS"
BASE COUNT          786 a          381 c          372 g          609 t
ORIGIN      
        1 ctttaggtct gctaaaagca gtgacgcaaa ttatgtatac caggtttatc cggttattac
       61 aaacgatggt gtgtatttta tcagcctgga aggtgtattc agaactagta tttatgaaca
      121 gaaaggtgga atgaccatgc gaagtggttc aactgaattg cgtctaagtt tacacgattt
      181 aaataaaggt acattaatta aacgcgaagt aatcggcgat tatcacaaaa tgtattctag
      241 cataattggt gctcgtgata gtattttttg gatgtacaac acaactgatg gtttacacgg
      301 aagaagtgta aaagcgctcg agactataat aacacaggag cagttaattg cttctaatcc
      361 tgccttatca gaaggcctgg ctatgtcgca atccacatta actaatctca gcgaattata
      421 tgctttcgac agagaacata acgcattaat gctaacaaca ataagtggta aaaagatttg
      481 ggtagatggc gcttctttta aaacaattga atcaccaatt gcctatttac caaaaaatag
      541 ttacgccgat ataattgaac gcaccatcga acaagcactg gcaggagaaa ctattgatgt
      601 caatttgatt actgaacaaa tattacaagc aacagcgggc gttaatacac gttttgaact
      661 tgaccacctt tcagacagaa ttgttgcgtt tgacagttgc acctataagt tagagggtgg
      721 cacactaaga gcaattacta aaacagattg ccctaaacca atactgcgga atacaaattc
      781 agcaggtgat gtcaaattta tcgaaccaca tcttatcagt acttacaata gcgaaaaaaa
      841 gcttgcagtt aatccggaat ttttgccggg caacatgagt attattactc atgctaaatc
      901 aataggcgac aaaaccgaat tattgattag tggaattaat aataccaatt tacaaaaaca
      961 attcaccatt gcaaccggta tcacattaag caactatcgt tcaggttata aaataagtgc
     1021 tgtttacggt agtcaggata ctttattatt gggtataaat aatcagcttt ttaatatcaa
     1081 taccaaaacc ggtaaactca tttggaaaaa agtaattgca aaagaagatt acaatgcaca
     1141 acttttatct gttggtgaag ctgtccaaaa cggcaaacga tacattgtag tggtaaacaa
     1201 ttactttaca gttttaagca aacaaggcat atttgttaat ggtagaaccg actatcaaca
     1261 acttgtaata gatgccgcca gcggtaaaat attacatcaa caggacatta ataatagtca
     1321 gccggaaaaa ctaccttatt accttggaaa tgcacatgga ttaaattggt tttataacga
     1381 tgaaatcgga ttccacacgc gctcatttcc taaattaata cctgaacaaa acgactttac
     1441 atcatcatta aaggtagcag aaatcacttc ccctttggtt aaaactagtg gatacggacg
     1501 cgcaattgat gataaataca tatcatttga tgcagcaaaa aaattatgtt acttcactac
     1561 cgaaaatgga ttgcattatg tttatgatat tactaataaa aaaataagtg aaataaatgc
     1621 accggatgat gccttatatc ataacctaaa aatgcaaaac ctcaatgtaa attattatta
     1681 tagaaacgct tactactcaa cacctgatag aattgaactt ccaaacggat gtgcattaac
     1741 ggcaaaaaat gaaaatgcaa ttcctgtttt acgatatgag gctgcgcaaa aatcatcacc
     1801 aactgcgtca acaacaacta gcactcaatt tattgaaggg gaatttttga taaatggtat
     1861 tagtttaaac aaggatatat tctatactga taaaaattac aacccgataa gcacgaataa
     1921 taaagaatcg ttttgttata tctggcataa aaataaaatc gctgtagatg cacatcggat
     1981 tatcagcaaa tacaactata ccacacaaca atcagcatgg caatttgatg taactgcact
     2041 tttgggtgtt gaaggtgaaa tttccagggt ttacaaccgc aagtcgagtt tggtaatcat
     2101 attcaaaaca catccaaaac tcgatgacaa tttcacctgt gtttcaat
//
LOCUS       DAORKW010000604         2138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3224_length_2138_cov_3.156025, whole genome shotgun sequence.
ACCESSION   DAORKW010000604 DAORKW010000000
VERSION     DAORKW010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3224_length_2138_cov_3.156025"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="PLB46_15995"
     CDS             complement(<1..572)
                     /locus_tag="PLB46_15995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRF78081.1"
                     /translation="MINSKSTNINILLYHQIGDAPDSETNPNCFCSVKEFSNQMFFLK
                     NSNYKVISLNEAMDMIFKINNIDANYVVLTFDDGCEKFYDVTFPVLEKFNFPSTIYPL
                     AGFLGKHAIIKGHEYKSLKILSQSMLLELNQLGVEIGAHTMNHVKLAQVDNDTAITEV
                     KTSKDTSEQVIGTKINSFSFPHGDYNSDVI"
     gene            complement(573..1754)
                     /locus_tag="PLB46_16000"
     CDS             complement(573..1754)
                     /locus_tag="PLB46_16000"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRF78082.1"
                     /translation="MDSKIYVKPKIILHCQYVYGIGHFVRALELASGLSKYFEVYIIN
                     GGESVPNFALPETVKYIQLPAIYKEENSDFLLSVDSSFTIEECFLLRKNIIQETVLNV
                     IPDILITEHFPFGLLFENEVIELITEVKKSNPLSKVVSSVRDIIESSGGGEKDEYISE
                     LINKWYDLILVHGDERMADLTFSFSKVAKVAVPIFHTGYIVRQIPNKEISADYPLVLV
                     SVAAGRLGNELLDAIIDSHLLIKEKIKHKLVLFSGAFQKDFSSLKEKVYSLASLDINI
                     HCFDGNEYLKYLSNASLVISLGGYNSIIESIAAKKTMLVYQRGFSGVNKEQDLRITLF
                     EKYGCLSILKPEDLDKESLSNIIVSMIEDINSTKIELNFDGVKNSINALLKLNNDYKY
                     I"
     gene            complement(1763..>2138)
                     /locus_tag="PLB46_16005"
     CDS             complement(1763..>2138)
                     /locus_tag="PLB46_16005"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRF78083.1"
                     /translation="KNCNVLVCQLPLTSETKGILNTHLFNFLPDASFLINVGRGGHLV
                     ESDLILALENKKLSGACLDVLEVEPIPLNHPFNKYPNIKVTPHVAGYIGPDTQAPYAS
                     KVIKDFFNNDIIKGVVNYNTFY"
BASE COUNT          695 a          369 c          321 g          753 t
ORIGIN      
        1 tcgataacat cactattgta atccccgtgt ggaaaagaga acgaattgat tttagtgcct
       61 ataacttgtt ctgatgtatc cttactcgtt ttaacttcag taatcgctgt atcattatct
      121 acctgagcca acttaacatg attcattgta tgagcaccaa tttcaacacc cagttgattt
      181 agttctaaaa gcatactttg tgaaaggatt ttaagacttt tatattcatg accctttatt
      241 atggcatgtt tacccaaaaa tccggctaat ggatatattg ttgaagggaa attgaatttt
      301 tctaaaactg gaaaagtgac atcataaaac ttttcacagc catcgtcaaa cgttaataca
      361 acataatttg catcgatatt atttatttta aaaatcatat ccattgcttc atttaaggat
      421 atcactttgt aattagaatt tttaagaaaa aacatttgat tagaaaattc tttaacgctg
      481 caaaaacaat taggatttgt ttcagaatca ggagcatcac caatttgatg atataatagt
      541 atgtttatgt ttgtgctctt tgagtttatc atctaaatat atttatagtc gttgttaagt
      601 ttaagtaagg catttatcga gttcttaaca ccgtcaaagt ttaattcaat tttagttgaa
      661 tttatatcct caatcatgct tactataata tttgacaagc tctctttgtc cagatcctct
      721 ggttttaata tggacaagca gccgtatttt tcaaataatg taattcttaa atcctgctct
      781 ttattaactc cggaaaaccc tctttggtag actaacattg ttttttttgc tgctattgac
      841 tcaattatgg aattgtagcc accgagtgaa ataactaggg aagcatttga tagatatttt
      901 aaatattcat tgccatcaaa acaatgaata tttatatcca atgaagcaag agaataaacc
      961 ttttctttta aactactgaa atctttttga aaagcaccgg aaaaaagtac gagcttgtgt
     1021 tttatctttt ccttaattaa taaatgacta tcaattatgg catccagtaa ttcattgcca
     1081 agacgacccg cagcaaccga taccaggaca agagggtaat cagctgaaat ttccttgttt
     1141 ggtatttgtc ttacaatata gcccgtatga aaaattggga ctgctacttt tgcaactttc
     1201 gaaaaactaa atgttaaatc tgccattctt tcatcaccat gaacaaggat taagtcatac
     1261 catttattta tcaattcaga tatatactca tctttttctc ctcctccact tgattctata
     1321 atgtcccgaa cgcttgatac tactttggat aatggattag atttctttac ttctgtaatc
     1381 agttcaatca cttcattttc gaaaagtaaa ccaaagggga aatgctccgt aattagaatg
     1441 tcaggtataa cattcaatac cgtttcttga ataatatttt tgcgaagaag aaaacattcc
     1501 tcaatggtaa atgatgaatc tactgataat aagaagtctg aattttcctc tttatatata
     1561 gctggtaatt gtatgtattt caccgtttct ggcaaggcga aattgggtac actttcgcca
     1621 ccattgatta tgtatacttc aaaatattta cttaaaccac ttgccagttc aagtgctcta
     1681 acgaaatgcc ctatgccata tacatattga cagtgaagaa taattttcgg tttaacatat
     1741 atttttgaat ccataaacag ttttaataaa aggtattata atttactacc cccttgatta
     1801 tatcgttgtt gaaaaagtct ttaatcacct ttgaagcata aggtgcttgg gtatctggcc
     1861 ctatatatcc agcgacatga ggagtaactt ttatattggg atatttatta aatggatggt
     1921 tcaatggtat aggttccact tctaaaacat caaggcatgc acctgatagc tttttgtttt
     1981 ctaaagcaag aataagatca gactctacca gatggccacc cctgccaaca ttaattaaaa
     2041 aagatgcgtc aggtaaaaaa ttgaaaaggt gtgtgtttaa aatccctttt gtttcactcg
     2101 ttaatggcaa ttgacacact aaaacattgc aattcttt
//
LOCUS       DAORKW010000605         2131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3238_length_2131_cov_4.902216, whole genome shotgun sequence.
ACCESSION   DAORKW010000605 DAORKW010000000
VERSION     DAORKW010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3238_length_2131_cov_4.902216"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PLB46_16010"
     CDS             <1..1063
                     /locus_tag="PLB46_16010"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRF78084.1"
                     /translation="VKNADAIQVVFYDKHTAVAELVATDPSTDIAVLKVDMKDLPNVK
                     YGDSDSVRVGDWVMAVGNPFDLASTVTAGIVSAKGRKIDILGENASTPVESFIQTDAA
                     VNPGNSGGALVNLKGELIGINTAIATPTGTFAGYSFAVPVNIVKKVVADLKKYGIVQR
                     AYLGIEVDVTKEDVGGVYVSNVVDGSGAKDADIKVGDIVTHINNAPINSFPELQEQLS
                     KYGPGEKVNVSILRNKERKNVVVQLKNQKGNTEIVKKEINEVVKSLGIETEDVSGKEA
                     DYYNVDGGVKVTGIKNGKIYQQTRIKEGFIITAIDDKKIVNYNDLAKILSDKSGKNVI
                     VEGFYPSFPNKIYNYGLSL"
     gene            complement(1077..1382)
                     /locus_tag="PLB46_16015"
     CDS             complement(1077..1382)
                     /locus_tag="PLB46_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78085.1"
                     /translation="MKSRNQLFFWVNKNLSYAKIAGGDLKGCFLEDIVGTQIDKGERR
                     LEKRPSRFKDLPGLNLEGFRGYDKISGLKTHSFSLKKKPRRSRAETKRSSHLKTQLP"
     gene            complement(1453..>2131)
                     /locus_tag="PLB46_16020"
     CDS             complement(1453..>2131)
                     /locus_tag="PLB46_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRF78086.1"
                     /translation="TLAMSAGQFNCPIVFRGPSGSAGQLGAQHSQMFENWYANVPGLK
                     VISPSNPYDAKGLLKSAIIDNDPVIFMESEVMYSDMGFVPEEEYYIPIGKADIKRVGS
                     DVTIVSFNKMMKVALKAAEELEKENISAEVIDLRTIRPLDIATIVESVKKTNRIVVVD
                     ESWPFGSIASEIGYQLQKDAFDYLDAPVRRVNGADTSMHYAGNLVNAYLPDPAKVIKV
                     VKEVMYR"
BASE COUNT          719 a          354 c          404 g          654 t
ORIGIN      
        1 ggtaaaaaat gcagatgcga ttcaggtagt attttacgat aaacatactg cagtagcaga
       61 attagtggca acagacccat caactgatat tgcagtatta aaagtggata tgaaagattt
      121 accgaatgta aaatatggcg attcagattc cgttcgcgtt ggtgattggg taatggcagt
      181 gggtaatcct tttgatttgg caagtacggt tacagctgga attgtgagtg caaaaggaag
      241 aaaaattgat attcttggag aaaatgcttc tacacctgtt gaatcattta ttcaaacaga
      301 tgcagcagtt aacccaggaa acagtggtgg agcattggta aatttaaaag gtgaattaat
      361 tggtataaat acagccattg ctacacctac aggaaccttt gcaggatact cttttgcagt
      421 tccggtaaat atcgttaaaa aagtagttgc agatttaaaa aaatatggaa ttgttcaacg
      481 tgcatactta ggcattgaag ttgacgttac taaagaagat gttggaggtg tttatgtaag
      541 taatgttgtt gatggttcag gtgcaaaaga tgctgacatt aaagttgggg atattgttac
      601 ccatattaac aacgcaccaa taaattcttt ccctgaatta caggagcaat taagtaagta
      661 tggacccggt gaaaaagtta atgttagtat tttacggaac aaagaaagaa aaaatgttgt
      721 tgtacaacta aagaaccaaa aagggaatac agaaatagta aagaaagaga taaatgaagt
      781 cgtaaaaagt ttaggtattg aaactgaaga tgttagcgga aaagaagcag attattataa
      841 tgttgacggt ggcgtaaaag ttaccggaat aaaaaatgga aaaatttatc agcaaactag
      901 aattaaggaa ggatttataa ttacagcaat tgatgataaa aaaattgtaa attataacga
      961 tttggcaaag attttgagtg ataagagtgg aaagaatgta atagttgaag gtttttatcc
     1021 ttcatttccg aacaaaattt ataattacgg gttaagtcta taatgatatt taagttttag
     1081 ggtaattggg ttttcaaatg tgagctccgc ttagtttccg ctcgggaacg gcggggcttt
     1141 tttttgagcg aaaatgagtg agttttaagg cctgaaattt tgtcatatcc tctaaaacct
     1201 tccaggttta aacctggaag gtctttaaac ctggaaggtc ttttttctaa gcgcctttcc
     1261 cctttatcga tttgcgttcc aacaatatcc tctaaaaaac aacccttcaa atcaccacct
     1321 gctattttgg catagctcag atttttatta acccaaaaaa ataattgatt tcgagacttc
     1381 aattcaggta aaaaaaaacc ggcatttaaa aaatgccggt tagcttgaat gaattattta
     1441 attggataaa tattatctat acatcacctc tttcactact ttaataactt ttgccggatc
     1501 aggtaaataa gcatttacca aatttcctgc gtaatgcatg ctggtatctg ctccgtttac
     1561 gcggcgaacc ggtgcatcta aataatcaaa tgcatctttt tgtaattgat atccgatttc
     1621 tgaagcaata ctgccaaacg gccagctttc atctacaaca acaatacgat tagttttttt
     1681 aaccgattca actattgttg caatatctaa aggacgaata gtgcgcaaat caataacttc
     1741 tgcactgata ttttcttttt ctaattcctc tgctgctttt aatgcaactt tcatcatttt
     1801 gttgaacgat acaattgtta catcacttcc aacacgttta atatctgctt tgccaatcgg
     1861 aatataatat tcttcctcag gcacaaatcc catatcacta tacatcacct cactttccat
     1921 aaaaataaca gggtcgttat caataatggc tgatttcaat aaacctttcg catcgtaagg
     1981 attagatgga gaaattacct tcaaacctgg cacattcgca taccagtttt caaacatctg
     2041 tgagtgttgg gctcccaatt gtccggcaga acccgatgga cctctgaaaa caatcggaca
     2101 attgaactga ccggcactca ttgcaagcgt t
//
LOCUS       DAORKW010000606         2125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3258_length_2125_cov_5.094203, whole genome shotgun sequence.
ACCESSION   DAORKW010000606 DAORKW010000000
VERSION     DAORKW010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3258_length_2125_cov_5.094203"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1936)
                     /locus_tag="PLB46_16025"
     CDS             complement(<1..1936)
                     /locus_tag="PLB46_16025"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF78087.1"
                     /translation="MHRKGTLLVFFTVLLLCCGNRLQAQQYHLKNYSIDDGLAGISVT
                     SLLQDSRGYVWIGTEDGGISRFDGKTFVNYTKRDGVGDNSINCLFEDKTGNIWIGTKN
                     NGVTKFNGYEFFQYGDDVVKNIEKIYSDSSGNILIYSFPNLFKISGDSIVAASENNSN
                     TGLINFFRAGGPKAIRAFIDKQGNKWIATHTGIYTIDKKYVELENATDYRVQFILNPE
                     QPEEPATTIMQDREGNIWIGTAFSGLYLFYDGAFSNFNNIPGLRNNYITAIGATPTSL
                     LIGTTSGLKKVLVDTVTKQYYEAPLNINGFISTSRINCIYQNPNGNWYAADENNNIIY
                     YEGKYAVTTITTLEPNTQITALSEDVAGNLWIATNNQGVYIRTNSTWMHLTEEDSLSA
                     NNITCLFKDSQGMLWIGTQNNGVMTYNGKSFGRYTYFDNGLISNHISCITEDKSGLIW
                     FGSPDAGLCSYDGKGFSFYTENDVLTSNNVASLAFDSKGGLWVGLNEGLDYVSFNTDS
                     TVTAKHFDAYDGFLGIKNTNNTILIDPTGHVWLGTVDGLFRYNPDEDIITATKPLIEL
                     RNIRLFFETTDWGLYSDTTAGWYNLPVNVKLPYNQNHITFDFSAIFFSIHEKIKYQVL
                     LEGFEDDWQNIDHNTSMTYSN"
BASE COUNT          646 a          402 c          366 g          711 t
ORIGIN      
        1 aattagaata ggtcattgac gtattgtggt caatattttg ccagtcgtcc tcaaaccctt
       61 ccaacaatac ctgatatttt attttttcat gtatggaaaa aaatatggcg ctaaaatcga
      121 aggtgatatg attttgatta tatggcagtt ttacatttac cggtaaatta taccatcctg
      181 cggtggtgtc ggaatataat ccccagtcgg ttgtttcaaa aaacaaacga atatttcgta
      241 attcaataag tggttttgtt gctgtgataa tatcttcatc cggattgtag cggaataatc
      301 catctacagt acccagccac acatgtcctg ttgggtcgat taaaatagta ttgttggtgt
      361 ttttaatacc caaaaatcca tcgtaagcat caaaatgttt tgcggtaaca gttgagtcag
      421 tattaaatga aacataatct aaaccttcat taagtcccac ccataaaccg cccttgctat
      481 caaatgcaag tgaagcgaca ttattagaag ttaaaacatc gttttcagta taaaatgaaa
      541 atcctttgcc gtcgtagctg cacaatcctg catcgggact accaaaccaa attaatccgg
      601 atttatcttc agtgatacaa gagatatgat tgctaattaa tccattgtcg aaataggtat
      661 agcgcccgaa cgattttccg ttgtaggtca ttacgccatt attttgggtg cctatccaaa
      721 gcataccctg actatcttta aacaagcaag ttatgttatt tgcactcagt gaatcttctt
      781 ctgtcaaatg catccaagtt gaattggtgc gaatataaac gccttggtta tttgtagcaa
      841 tccataaatt accagctaca tcttcgctga gcgcagtaat ttgtgtattc ggttcgagtg
      901 ttgttatagt tgtaactgca tatttccctt cgtaataaat aatattatta ttttcatcgg
      961 cagcgtacca attaccattt ggattttgat aaatacaatt aattcttgag gtagaaataa
     1021 aaccgttaat attcaacggt gcttcataat attgtttggt aacagtatcg actaatactt
     1081 ttttaagtcc cgatgtagtt ccaattaata acgaagttgg tgttgcgcca atggctgtaa
     1141 tataattatt gcgcaagccg ggaatattat taaaattaga aaaagcacca tcataaaata
     1201 aatacaatcc gctaaatgca gtgccaatcc aaatattacc ttcccggtct tgcatgatcg
     1261 tagttgccgg ctcttcgggt tgttcgggat ttaaaataaa ttgcacgcgg taatcggtgg
     1321 cattttctaa ttccacatat tttttatcaa tggtataaat accggtatgc gttgcaatcc
     1381 atttattccc ctgtttatcg ataaaagcgc gtattgcttt tggacctcct gctctgaaaa
     1441 aattaattaa tccggtattg ctgttatttt cagatgctgc tactatacta tcgccactta
     1501 ttttaaataa attcgggaaa ctgtaaatta aaatattacc actgctatcg ctataaattt
     1561 tttcgatatt ttttactaca tcatcaccat attgaaaaaa ttcgtagcca ttaaatttgg
     1621 tcactccatt atttttggtg ccaatccaaa tgttacctgt tttatcttca aataaacaat
     1681 taatactatt atcaccaaca ccatcgcgtt tggtataatt tacaaatgtt ttgccgtcga
     1741 atcgtgagat accaccatct tcggtgccaa tccaaacgta accgcgtgag tcttgtaata
     1801 aactggtaac agaaatacct gccagtccgt cgtcgatgct ataatttttt aagtgatatt
     1861 gttgagcctg taagcgattg ccacagcata ataataatac ggtaaaaaaa acgagcaggg
     1921 ttcctttacg atgcatgtgg ttgaactgtt gcttagctgt ttggttgaac tgtaaagtta
     1981 atggtaatat catgttcagg aacatatccc ttttcgcgat aggtcacgtt tccgttgttg
     2041 tcgatacgta caaatgtagt taaacgcgtt ccataatccg gtgaagttat aaacattgcg
     2101 gacagctttt tctcccattc aaaac
//
LOCUS       DAORKW010000607         2122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3263_length_2122_cov_3.787131, whole genome shotgun sequence.
ACCESSION   DAORKW010000607 DAORKW010000000
VERSION     DAORKW010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3263_length_2122_cov_3.787131"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PLB46_16030"
     CDS             complement(<1..647)
                     /locus_tag="PLB46_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78088.1"
                     /translation="MKKVYFVLLLFYTTLNYGQSGSLAFTTSGTFTVPDGVTTILVEL
                     IGAGGHGGYNGTGGGGGGGYASSNLTVTPGETINIQIGIPGSGAVLGTTKIDNSFYAT
                     GGENGVSVPNPEIGGGGAGGIGVGGNVANYTGGNGGGGYYTYFGGGGGGAAGPGGNGG
                     IGGNTIAWVGICLTPGGDGGVSGGLPGGDGGKGAGFTDTFCAATDPAGNGNNYGAG"
     gene            complement(671..1390)
                     /locus_tag="PLB46_16035"
     CDS             complement(671..1390)
                     /locus_tag="PLB46_16035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRF78089.1"
                     /translation="MKAMITIKKSVYKILRKLGIVGKAYQLLPNNFPIANINNTFNQI
                     GKLHPRVTHPTEYVVLGTLASITKHNILEVGCYLGYSSCIMASAISGDRKIFSIDKFE
                     IPKNWSKNTSDNWIYKNYSQIEWATKNRHALGLETKITFVKSDSKTYAATLRNMPDRP
                     KFDLIFIDGDHTYDGCLSDMKDYMPMLEAGGYLVVHDYNSPLHPGVSKAVDEIIQSDK
                     SFKCLYLVQSLVVIIKEHPKN"
     gene            1641..2108
                     /locus_tag="PLB46_16040"
     CDS             1641..2108
                     /locus_tag="PLB46_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78090.1"
                     /translation="MKLLIGLLLLTNVAMAQVNNIGDISLNKPQITIPFALFAGICDG
                     LSQTLYAHYGAFENRFPNANDQYWNPYVSWTNKYENNDPNLGERFPGSTTLFVFTTDA
                     YHLFRTVNKVNLLTLGALEFSEKRPLLNYAIDFILYSAVYSAGFTLTYQVFFK"
BASE COUNT          673 a          446 c          338 g          665 t
ORIGIN      
        1 ccggcaccat aattattgcc attaccggct ggatcggttg cagcacaaaa cgtatcggta
       61 aaaccggcac ctttgccacc gtctccaccc ggtaatcctc cacttactcc cccatcacca
      121 cctggagtta aacaaattcc aacccaggca atagtattac caccgatacc accatttccg
      181 ccagggcccg cagcaccacc tccaccaccg ccgaaatagg tatagtaacc accgccgccg
      241 ttgcctccag tatagttggc cacattgccg ccaacaccta ttcctcctgc gcctcctcca
      301 cctatttcag gatttggaac agaaacaccg ttttcgccac ctgtagcata aaacgaattg
      361 tcaattttgg ttgtccccag tacagcacca ctgccaggaa taccaatctg aatgtttatt
      421 gtttcaccgg gagtaacagt taaattgcta ctggcataac caccacctcc accgccaccg
      481 gtcccattat aaccaccatg gcctccggca ccaatcaatt caactaatat agtagtaaca
      541 ccatcaggaa ctgtaaatgt gcctgatgta gtaaatgcta aactaccaga ttgaccgtaa
      601 tttaaggttg tataaaacaa taataaaacg aagtatactt ttttcatgta atatatttta
      661 aattaaaaaa ttaattttta ggatgttctt ttattattac taccaaggac tgtacaagat
      721 ataaacattt aaatgactta tctgattgaa tgatttcatc tactgccttc gaaacaccgg
      781 gatgtaatgg tgaattataa tcatgtacta ccaaataacc acctgcttcc aacattggca
      841 tataatcttt catatcgctt aaacaaccat cataagtatg atctccatca ataaaaatta
      901 aatcaaattt tggtctatcg ggcatgtttc gcaatgtagc agcgtaggtc tttgaatcac
      961 ttttaacaaa ggtaatttta gtttcgagcc cgagtgcatg acgatttttt gtagcccact
     1021 caatttgcga atagttttta taaatccaat tgtcactcgt gtttttcgac cagtttttag
     1081 ggatttcaaa tttatcaatt gaaaatattt ttctatcacc agaaattgca gaagccatta
     1141 tgcagctgga gtaacctaaa tagcagccca cctccaaaat attgtgtttg gttatactag
     1201 ccagcgtgcc taaaacaaca tattctgtag gatgcgtaac acgaggatgt agtttaccaa
     1261 tttgattaaa tgtattgtta atattggcga taggaaaatt attgggaagt aactgatatg
     1321 ctttaccaac aatgccaagt tttcgtaaga ttttgtaaac agatttttta attgtaatca
     1381 ttgccttcat atcatcatta ttaattcatg tgagtaatat cgcttttatg tataaatctt
     1441 attgctaaag gtaacagata ttttatactt aaaaaaataa ataatctgtc attttttgat
     1501 ggaaagcttg cccatgtgcc atgaggaggc aattaaattg catgaaatta ttcaaataaa
     1561 gttagaaaag tagttttgaa tatcgtttta ctttaagtaa tttagatttt cattcaggca
     1621 aattttaaat tctctaactt atgaaattac tcatcggact gcttctgctt acgaatgtcg
     1681 caatggctca agttaataac attggtgaca tatccttaaa caaaccacaa atcactatcc
     1741 catttgcact ttttgcagga atttgcgacg gtttatcaca aacattatat gctcactatg
     1801 gtgcattcga aaataggttc ccaaatgcca atgatcaata ttggaatccg tatgtgagtt
     1861 ggacaaacaa atatgaaaat aacgacccga atttaggtga aagatttcct ggctcaacaa
     1921 ccctctttgt atttactacc gatgcttatc atttgtttag gacagtaaac aaagtaaatc
     1981 tattaacctt aggcgcattg gaatttagtg aaaaacgacc cctcctgaat tatgcgatag
     2041 atttcatttt atattctgct gtgtatagtg caggcttcac cttaacatat caggtatttt
     2101 ttaaataaac gtgaagtttg cc
//
LOCUS       DAORKW010000608         2113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3281_length_2113_cov_5.349854, whole genome shotgun sequence.
ACCESSION   DAORKW010000608 DAORKW010000000
VERSION     DAORKW010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3281_length_2113_cov_5.349854"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="PLB46_16045"
     CDS             <1..1135
                     /locus_tag="PLB46_16045"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRF78091.1"
                     /translation="DDHVDLNYGKYPFSDRIMYDSEEHNRTNVTVKTAFALSSNVGIS
                     RLANQMYQKEPSKYTKHLIESGITKETGIELAGERKPVFKLDPKNKNEWYGTTVPWMS
                     VGYELELTPLQLLTYYNGIANDGKMMKPYLVQETQQYGVTLQETEPVVLNEKLASEST
                     IKKLQECLLAVVDSGTAKHLKNDYYQFAGKTGTAKVVENGVYSDKYLASFAGYFPYDV
                     PKYSIVVMVHSPSNHVYYGGAVAAPVFREIADKVYSHFINIREPINNTNTQYVGVDAK
                     ARGYAYDFRQILSWLHVSEGFDDNEEWVAINAHGKSASHENIKTYQYTMPDVRGMGLR
                     DALYLLESNGLKVGVNGAGKVVAQSITPGQAVNKGTYVSIQLN"
     gene            1132..>2113
                     /locus_tag="PLB46_16050"
     CDS             1132..>2113
                     /locus_tag="PLB46_16050"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRF78092.1"
                     /translation="MKQLNNILSVLGNIAIANDIPIAGITSDSRQVKPGYVFVAVKGT
                     TVDGHQFIDKAIASGASAIIAENRAGIVTELPFIIVKNAAESLGKLAHAFYDFPSTQI
                     KLIGITGTNGKTTTATLAYHLLRDLGFKVGLISTVENKIHDTIIPSTHTTPDPVQLNA
                     LLAQMVDAGCDYVFMEVSSHAAHQHRIAGLQFTGGVFTNITHDHLDYHKTFSHYLSAK
                     KMFFDALPKTAFALVNSDDRNGEVMLQNCAAAKYTFSVKGPANFKAQIKENVITGLVL
                     NLDGAEFHSNLLGEFNAWNLVSVYAIGILLGFNKNEVLTALSKQKPVEGRF"
BASE COUNT          728 a          347 c          418 g          620 t
ORIGIN      
        1 agacgaccat gtagatctaa attatggcaa ataccctttt tctgaccgca tcatgtatga
       61 tagtgaagaa cacaatcgca ccaatgtaac agtaaaaact gcatttgcac tttcctctaa
      121 cgtgggtatt tctcgtttgg cgaatcaaat gtatcaaaaa gaaccatcaa aatataccaa
      181 acatttaatt gaatccggta ttacaaaaga aacaggaatt gaacttgcag gtgagcgtaa
      241 gccggttttt aaactggacc cgaaaaataa aaatgaatgg tatggaacaa ccgtgccgtg
      301 gatgagtgtg ggttatgagt tggaattaac gccattacaa ttattgacct attataatgg
      361 tattgcaaac gatggtaaaa tgatgaaacc ctatttggtg caggaaacgc aacagtatgg
      421 tgtaacatta caagaaactg aaccggttgt attaaatgaa aaactcgcaa gtgagtcaac
      481 cattaaaaaa ttacaggaat gtttattggc tgttgtggat agtggaactg ccaaacattt
      541 aaaaaatgat tattaccaat ttgccggtaa aacaggtact gctaaagtgg tagaaaacgg
      601 tgtgtattca gataaatatc tcgcaagttt tgccgggtat tttccttatg atgtgccaaa
      661 atattctatt gtagtaatgg tgcattcacc aagtaatcat gtttattatg gcggcgcagt
      721 tgcagcaccg gtattccgtg aaattgcaga taaagtatat tctcacttta ttaatattcg
      781 cgagccaatt aataatacaa atacgcaata tgtaggtgtt gatgcaaaag cacgcggata
      841 tgcttacgac ttcagacaaa tattaagttg gttacacgtg agtgaaggat ttgatgataa
      901 tgaagaatgg gttgctatta atgcacatgg aaaatcagca tcacatgaaa atataaaaac
      961 atatcagtat accatgcctg atgtaagagg tatgggatta cgtgatgcat tatatctatt
     1021 agaatcaaat ggattaaaag ttggtgttaa tggcgcaggc aaagtagttg ctcaaagcat
     1081 tacaccgggt caggcagtaa ataaaggaac atacgtatcg atacaattaa attgaaacag
     1141 ttaaataaca tattatccgt tttaggcaat atagcaatag caaacgatat ccctattgca
     1201 ggtatcactt ccgacagtcg tcaagtgaaa cccggatatg tatttgttgc cgttaaagga
     1261 acaactgtcg acggacatca atttattgat aaggcaattg cttcaggtgc cagtgctatt
     1321 attgctgaaa acagagcagg aattgttact gaattaccat ttattattgt taaaaatgct
     1381 gctgaatcgt tgggaaaatt ggcacatgca ttttatgatt tcccttccac gcaaataaaa
     1441 ttaattggta ttacaggtac taacggcaaa accacaacag caacacttgc ttatcattta
     1501 ttgcgcgatt taggatttaa agttggatta atttcaactg tagaaaataa aattcatgat
     1561 actatcatcc cttcaacgca tactacaccc gacccggtgc aattaaatgc gttgttagcg
     1621 caaatggtag atgcgggatg tgattatgta tttatggaag tgagttcaca tgcagcgcat
     1681 caacaccgta ttgcaggatt acaatttacc ggtggcgttt ttaccaatat tacccacgac
     1741 catttagatt atcataaaac atttagtcat tacctcagtg ctaaaaaaat gttttttgac
     1801 gcattaccaa aaacggcttt cgctttagta aacagcgacg accgcaatgg agaagtaatg
     1861 ttacaaaatt gtgctgcagc taaatatact ttttcagtta aaggacctgc caattttaaa
     1921 gcacaaataa aggaaaatgt aattaccgga ctggtattaa atttagatgg tgctgaattt
     1981 cattccaatt tattgggaga atttaatgca tggaatttag tgtccgttta tgctataggt
     2041 atattattgg gatttaataa aaacgaagta cttactgcac tcagtaaaca aaaacctgta
     2101 gaagggcgct ttg
//
LOCUS       DAORKW010000609         2112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3286_length_2112_cov_3.234808, whole genome shotgun sequence.
ACCESSION   DAORKW010000609 DAORKW010000000
VERSION     DAORKW010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3286_length_2112_cov_3.234808"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..1524
                     /locus_tag="PLB46_16055"
     CDS             388..1524
                     /locus_tag="PLB46_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78093.1"
                     /translation="MYSSTTTSLSISQHYDPFGMLLEGRNFEADYRFGFQNQEVDEEF
                     WGGAVSFKYRVEDARLGRFFAVDPLSNSYPWNSPYAFCENKVGLGIELEGAELQELNS
                     SFFNMKGSGGFTGLGYGVNGPVLCKPTAYEIAIVNRNVPEPLKYADGTLRFSVGSLDM
                     TPTGGSLAAENPYGVITSQSDYHSPDWSWNQYNVENALSISDQGVYPHYNQPKNYPNS
                     GFYMSVDADIEAGLNQSTSPKVYGKVYNSSGLGNAPQEFGSWVKMYQSIPIWLAIGDL
                     GNNKRNFNIAINLVDNYIALNTTDILATLNISNIPGAFRANMINYVMDGTLPKITDET
                     GKVNSDQLRWNLYYQYYGGQALRGLWKQESVDKYNKDVNLWEGN"
     gene            1539..2045
                     /locus_tag="PLB46_16060"
     CDS             1539..2045
                     /locus_tag="PLB46_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78094.1"
                     /translation="MKIIYTFIFCSFILFISCDEKKRDRLTTAGKLIQYLDNSNVEAI
                     DSLITSNRTFDRPDIERLTHDCLYFNQLQIAVNYRNLKYTYIENDKDFVYPFKVIVPL
                     FEGIDTIGNRRITKVTLEVNFGPEWVHDAHHIAGYYINDEFDFIKSVDTLRRPNSNNF
                     QLKNPDGD"
BASE COUNT          686 a          333 c          402 g          691 t
ORIGIN      
        1 tatgaccatg cgtttttttt aaactgcaga gccagttcac gttaagttgt tttgcgcacc
       61 attattttct ttcccattaa cttacacatc aagtaaaact ttaaatgctt cagtcatgtg
      121 ctacgtaact tctttggata gcattacgac aacatcgtcg tgttaatatc tagccggacg
      181 gtcttaagcc cccgcccaac cacgtttact cattaaccca aaatacgcat tattcttttt
      241 tcccatacgc tttgaagagg gtatagaagc tcaattaatt ttgttttgga tggtcaatct
      301 aaaataagaa ttatattctc caattttgga gccattaaaa tcgcctgaat ttgctaatta
      361 tgcatttttt tttgttactt ttatttcgtg tattcttcaa ccaccacttc actttcaatt
      421 tctcaacatt acgacccgtt cgggatgttg ttggaaggaa gaaattttga agctgactat
      481 cgatttgggt tccaaaatca ggaagtggat gaagaatttt ggggtggtgc agtttcgttt
      541 aaatatagag ttgaggatgc aaggcttgga aggttttttg cagtagatcc tctttcaaat
      601 tcttacccgt ggaatagtcc atatgccttt tgtgaaaata aagttggatt gggtatagaa
      661 ttagaaggcg ctgaattaca ggaacttaac agttcatttt tcaatatgaa aggatccggt
      721 ggatttacgg gattagggta tggcgttaat ggccccgtat tgtgtaaacc aactgcttat
      781 gaaattgcaa tagtaaatag aaatgttccc gagcccctaa aatatgctga cggaactctg
      841 agattttctg tagggagctt agatatgaca ccgacaggag gaagcttggc tgcagaaaat
      901 ccttatggag taataacttc acaatcagat taccattctc cagattggtc ttggaaccaa
      961 tacaatgttg aaaatgcttt aagcataagt gaccagggag tatatccaca ttataatcaa
     1021 cctaaaaatt accctaactc cggattttat atgagtgtgg atgcagatat cgaagcagga
     1081 ttaaatcaat caacgtctcc taaagtttac ggtaaagttt acaatagttc tggtttagga
     1141 aatgcacctc aagagtttgg aagctgggtt aaaatgtatc aatcaatacc aatttggtta
     1201 gcaattggtg atttgggaaa caataaaagg aattttaata tagcaattaa tttggtagat
     1261 aattacattg ctttaaatac tactgatatt cttgccactc taaatattag taatatccca
     1321 ggggcgttta gagccaatat gataaattat gtaatggacg ggacactacc caaaataaca
     1381 gatgaaacag gtaaggtgaa ttctgatcaa ttgaggtgga atttatatta tcaatattat
     1441 ggtggacaag cgctcagagg tctatggaag caagagtcag tggacaaata taataaggat
     1501 gttaatttat gggaaggtaa ttaatactta atgattttat gaaaataatt tatactttca
     1561 ttttttgtag ttttatttta tttatttctt gcgatgaaaa gaagcgcgat aggctaacaa
     1621 ctgcaggcaa attgattcaa tatcttgaca attcaaatgt tgaggctatt gatagtttaa
     1681 taacaagtaa tcgcactttt gataggcctg atattgagcg attaactcat gactgcctat
     1741 attttaatca acttcaaata gccgttaatt acagaaattt aaaatatacc tatatcgaaa
     1801 atgataaaga ttttgtctat ccatttaagg ttattgttcc tctctttgaa ggcatagaca
     1861 ccattggaaa cagaagaata acaaaagtaa cgctcgaagt taattttgga cctgagtggg
     1921 tgcacgatgc acatcatatt gcgggatatt atattaatga tgagtttgac ttcattaaat
     1981 ctgttgatac attgaggaga cctaattcta ataatttcca actaaaaaac cctgatggtg
     2041 attaaaaact aaaaattgct tagtttataa atgcatgaga actatgactg acaagattgg
     2101 gtattcacta gt
//
LOCUS       DAORKW010000610         2110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3288_length_2110_cov_4.341119, whole genome shotgun sequence.
ACCESSION   DAORKW010000610 DAORKW010000000
VERSION     DAORKW010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3288_length_2110_cov_4.341119"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLB46_16065"
     CDS             complement(<1..917)
                     /locus_tag="PLB46_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78095.1"
                     /translation="MNPTVFLYPTMKFVKTLLKVKVDWLLLIFFLLLILLPFILFSAP
                     AATPPQPATLPYVMPDEIPAFLVTGELDTIQEDFPGIYTSRFIALATIPTDTVYVIIT
                     PNEEINVGAGIGLVDTLMFTPYAAALIAKEIKIKPYDDTEYEGEHYGVMHFTILTDDP
                     EYAALTITSDSVLILDNDLPPGITTSFPSDTSYTEGLAGFDYLIALTSIPTDTVYITI
                     DPDDQLRITGIPGEPIVLTFEPNASALSYDGASVRAVDDAIYEGLHTGSVTFSITTDA
                     PEYTAFVIPEVVLPIIDNDSMPGINFVIPA"
     gene            1073..1687
                     /locus_tag="PLB46_16070"
     CDS             1073..1687
                     /locus_tag="PLB46_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78096.1"
                     /translation="MNVTVLRVLLFAMIFVSVSTVFSQTQPIESDEQSSSVFEGNTDN
                     SDNERKTWKDRIVVGGSLGATFGSSTYVEVSPIVGYKVTDKFTAGLGFSYQYYKVNYN
                     VFTIDDYTMSVYGPRIFAQHDIIYGLFAHAEYEMMKYNFKYEDPLFGEYTGQVPALFG
                     GLGYNYAIGDKAKFQLTVLYDFLNGPNSLYYSPWVFRMGFSTGF"
     gene            complement(1716..>2110)
                     /locus_tag="PLB46_16075"
     CDS             complement(1716..>2110)
                     /locus_tag="PLB46_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588067.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRF78097.1"
                     /translation="TLILTVFFRKWYELVQQGYIYIATPPLYLVKKGKEQQYCWNEED
                     RKAATAEFAKGGSENSVHVQRYKGLGEMNAEQLWETTMNPATRTLRQVTIDSAAEADH
                     IFSMLMGEDVPPRREFIEANAKYANLDI"
BASE COUNT          626 a          388 c          452 g          644 t
ORIGIN      
        1 gcaggtatta caaaattaat tcccggcata ctatcgttgt cgataatggg gagaacaacc
       61 tccggaataa caaaagcagt gtattcaggc gcatcagttg ttatactaaa tgtaactgag
      121 ccggtatgca atccttcata aatggcatca tcaacagcgc gcacacttgc cccatcgtag
      181 gataaggctg aggcatttgg ctcaaaagtt aacactattg gttcacccgg tataccggta
      241 atacgcagtt gatcatcggg atcaatggta atataaacgg tgtcggttgg aattgaggtt
      301 aatgcaatta aataatcaaa acctgcaagc ccttcggtgt aactggtatc agatggaaac
      361 gaggtcgtaa ttccgggagg aaggtcgtta tcgagaatta gtaccgaatc ggatgtgatt
      421 gtgagtgctg catattcagg gtcatcggta agaatagtga agtgcataac accataatgt
      481 tcaccttcgt attcggtatc atcgtatggt tttatcttaa tttctttggc aataagtgct
      541 gctgcataag gcgtaaacat aagtgtgtca accaagccaa ttcctgcacc cacattaatt
      601 tcttcatttg gggtaatgat gacgtacaca gtatcggttg gaatagttgc caatgcaata
      661 aagcgcgacg tataaatgcc agggaaatct tcttgaatag tatccaattc gcctgttacc
      721 aaaaaagcgg gtatttcgtc gggcatcaca tacggtaaag ttgccggctg cggaggtgtt
      781 gctgcagggg cagaaaaaag gataaaaggg agtaaaatga ggaggaggaa gaaaatgagc
      841 aatagccaat ctactttcac tttcagcaat gttttcacga atttcatagt cggatataaa
      901 aatactgtgg gattcataac aaatctatcg gttttgagca aagttgaggt tttttcctgt
      961 gaaacgccct aactaattag cgaagtaaaa aatacggaac ggtttttgac cgttaaaaaa
     1021 cattctgaac gagtttaatt gctaatttta ccattgataa ttattgtata atatgaatgt
     1081 aacagtattg cgcgttttac ttttcgccat gattttcgtt tccgtttcaa ctgtatttag
     1141 tcaaacccaa cccattgaat ctgatgaaca aagttcatcg gtatttgaag gtaatactga
     1201 taacagtgat aatgaacgta aaacctggaa agaccgaatt gttgttggtg gtagtttggg
     1261 tgcaacattt ggcagttcca cctacgtaga agtttcacca atagtgggtt ataaggtaac
     1321 cgataagttt accgccggtt taggattttc ataccagtat tacaaagtaa actacaatgt
     1381 ttttactatc gatgattaca ccatgtcggt atatggtcca cggatttttg cacagcatga
     1441 tatcatttat ggtctttttg cacatgcgga atacgaaatg atgaaataca atttcaagta
     1501 tgaagacccc ttatttggcg aatatacagg tcaggtgcct gcactttttg gcggtttagg
     1561 atacaattat gccattggcg ataaagctaa atttcaatta acagtattgt atgattttct
     1621 gaacggacca aattctttgt attacagtcc ctgggtattc cgtatgggtt tcagcaccgg
     1681 tttttaacca atgctgaaac ttcggaaata atgttttaga tatcgaggtt agcatatttc
     1741 gcattcgctt caataaactc tctgcgaggc ggaacatctt cacccattaa catagaaaat
     1801 atatggtcgg cttcggctgc gctatcgata gttacctggc gtaaagttct ggtagcaggg
     1861 ttcatggtag tttcccataa ttgttctgcg ttcatctcac ccaaaccttt atatcgttgt
     1921 acgtgaacag aattttcact accgcctttt gcgaattcgg cagtggcagc tttacggtcc
     1981 tcttcattcc aacaatattg ttgttccttt ccttttttca ctaaatacag tggaggtgtg
     2041 gcaatataga tataaccttg ttgcactaat tcataccatt ttctgaagaa gacagtcaga
     2101 ataagtgtgg
//
LOCUS       DAORKW010000611         2102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3305_length_2102_cov_3.490962, whole genome shotgun sequence.
ACCESSION   DAORKW010000611 DAORKW010000000
VERSION     DAORKW010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3305_length_2102_cov_3.490962"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLB46_16080"
     CDS             <1..202
                     /locus_tag="PLB46_16080"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HRF78098.1"
                     /translation="KDYGFVVPKDITGETVVIEGKAEIREVSVDEQRHLAADAGKSQK
                     EIDAITTTKQELRFVADGVLIK"
     gene            374..446
                     /locus_tag="PLB46_16085"
     tRNA            374..446
                     /locus_tag="PLB46_16085"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:407..409,aa:Lys,seq:ttt)
     assembly_gap    486..585
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            897..>2102
                     /locus_tag="PLB46_16090"
     CDS             897..>2102
                     /locus_tag="PLB46_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78099.1"
                     /translation="MCLTRVYGQAPEIEWQKTIGGYNDDNLTCIRQTADGGYILGGDS
                     YSGANGDKTENKFGDSDYWMVKLDASGNIVWQNTIGGSSIEVLNALEQTLDGGYIIAG
                     YSLSPISGDKTESNILGSYDYWIIKLDAAGSIVWQNTIGGNGNDKANCIIQTIDGGYL
                     VGGESASDISGDKSENSLGGTDYWVIKLDASGNIVWQNTIGGVSYDGLFSIDQTVDGG
                     YILGGYSISDISGDKTEATMLGSYDYWIIKIDISGNIIWQNTIGGTASDKLKKVIMLN
                     DGNYIVGGTSASGISGDKTEVNHSDDYWVLKLDASGNIIWQNTIGGSGSDRLYDMDLS
                     ADGGYILGGSSLSNVGFDKSEACIGPSSMDDYWIVKLNSSGNIIWENTIGGNKEDNLR
                     SIDQSAEGGI"
BASE COUNT          653 a          303 c          452 g          594 t
ORIGIN      
        1 taaagattac ggctttgttg tgcctaaaga tattacaggt gaaactgtgg taattgaagg
       61 taaagctgaa atcagagaag tatctgttga cgaacagcgc cacttagcag ctgatgccgg
      121 caagagccag aaagagattg atgccattac aacaaccaag caggagttgc gttttgtggc
      181 agatggcgtg ctgattaaat aaataatttc cacaatattt ttatttacgg cagaaggttg
      241 tatctttgcg acacttttga aaaaaggggg tgcaaaatat tgcctcttct ttaaacaaaa
      301 caagagtgat tccgattccg tcccgatagc tatcgggatc agttggtaga gcataacact
      361 tttaattgtt ttcgattccg tagctcagtt ggtagagcat aacactttta atgttggggt
      421 cctgggttcg agtcccagcg ggatcactag aaaaaaataa aagtccgctt caaaggcgga
      481 tttttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacgag aagtttatcc
      601 cgacaatacg cagtgcaacg gagtattcgt cgggaagtcc cagcgggatc actagaaaaa
      661 aataaaagtc cgcttcaaag gcggattttt ttatgtactt tattaatcta ataggacgag
      721 aagtttatcc cgacaatacg cagtgcaacg gagtattcgt cgggaagtcc gaacgggatc
      781 acgaaaaaaa atactttacg aatttgctat ggaattatat attccaaagt ccgatccttt
      841 tccttaaatt tacttccatg aagcactcca taaaatactt attcctaatt ttattaatgt
      901 gcttgactcg tgtttacgga caggctcctg aaatcgagtg gcaaaaaacc attggtggct
      961 ataacgatga taatttaact tgcattagac aaactgctga tgggggttac attttaggtg
     1021 gtgattcata ttccggagcg aatggtgata aaacagaaaa caaatttgga gactcagatt
     1081 attggatggt aaaacttgat gcatcaggaa atatcgtttg gcaaaataca attggaggtt
     1141 cttcaattga agtgttgaat gccttagaac aaaccttaga tggtggatat ataattgccg
     1201 ggtactccct ttcaccgatt tcaggtgata aaacagaatc aaacattttg ggaagttatg
     1261 attattggat aataaaactt gatgcagccg gaagcatagt ttggcaaaac accattggtg
     1321 gaaacggaaa cgataaagca aattgtataa ttcaaaccat tgatggtggt tatcttgttg
     1381 gaggagaatc tgcctccgac atatcaggag ataaatctga aaatagtttg ggcggtacag
     1441 actattgggt tattaaactt gatgcctcag gtaatattgt atggcaaaac actattggcg
     1501 gagtatctta tgacggacta ttttctattg accaaacggt tgatggtgga tatatacttg
     1561 gcgggtattc aatttcagat atatcaggag ataaaactga agcaactatg ttgggaagtt
     1621 atgattattg gataattaaa atagatatat ccggtaatat aatttggcaa aatacaattg
     1681 gaggtactgc ctctgataaa ttaaaaaaag taataatgtt aaatgatggt aattacattg
     1741 tagggggcac atcagcttcc ggtatatcgg gagataaaac tgaggttaat cattcagatg
     1801 attattgggt attaaaactt gacgcttcag gaaatataat ttggcaaaat actattggtg
     1861 gatcaggtag tgacagatta tatgacatgg atttatccgc agacggtggt tatattttag
     1921 gaggctcttc gctttcaaat gttggttttg ataaatctga ggcttgcatt gggccgtcat
     1981 ctatggatga ttattggatt gttaaactta attcatcagg aaatattatt tgggaaaata
     2041 caataggtgg aaataaagaa gacaatttgc gttcaataga tcaatcagca gaagggggaa
     2101 tt
//
LOCUS       DAORKW010000612         2099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3312_length_2099_cov_4.216243, whole genome shotgun sequence.
ACCESSION   DAORKW010000612 DAORKW010000000
VERSION     DAORKW010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3312_length_2099_cov_4.216243"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLB46_16095"
     CDS             <1..201
                     /locus_tag="PLB46_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78100.1"
                     /translation="VLNTLNTAIVNPVTGEAYQYGDPVPSEWRDPSFIDPRDPRSYGT
                     PPDNPARYYEQRHILAGLSWKF"
     gene            293..1009
                     /locus_tag="PLB46_16100"
     CDS             293..1009
                     /locus_tag="PLB46_16100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78101.1"
                     /translation="MKKIITPLLFSLFYLFSNNLTAQLCNDIPISLVGAPFNLVASDV
                     ISYGDSMLTFELKNQHPTQFFAYPQAKLVALAPLPPGMSLATVNLDWMVFASAWNVDS
                     VALVRIYYNVEEPITENFTVPMQIWVNNLSPVTDSCYFSKNYVVNLNPTPMAVNTLES
                     QTLHIFPNPITANQPISIQNNQSLNLQQFTITNLVGELIKSGTIQNNTIPTTDLNKGF
                     YLLTILVDNGFKQTFKILID"
     gene            1010..1579
                     /locus_tag="PLB46_16105"
     CDS             1010..1579
                     /locus_tag="PLB46_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78102.1"
                     /translation="MKFTFLAVIVLTFCAAWISPQSEAHLIYHDNNVQLKWKDYQGTV
                     EEDANITALTSIGFRMGYKGTARQDSLIVVVEAFFDKQNSWVKKDTTAYALKHEQLHF
                     DITELYARKLKQSIQSASLTYKNYQSVMRDLERKAHNDMNTADSIYEVATDYSKNKVE
                     QKKWNDNIAVDLSNLKAFEADTFKVPVRF"
     gene            1679..>2099
                     /locus_tag="PLB46_16110"
     CDS             1679..>2099
                     /locus_tag="PLB46_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78103.1"
                     /translation="MSKFPKTLLIPLLVFMHGYCLAQTGDVYYMRADYDEPWCCGWAP
                     YEFSNPLNLTAVFGPGGWSLKYYEYTDANAIFNDNTCYVFMEGGDGHADMMEWFFSCN
                     QTLIEDWVYNGGSLFINAAPNVGDGLSFGFGDVWLWYP"
BASE COUNT          661 a          402 c          378 g          658 t
ORIGIN      
        1 gtgctgaata cattaaatac cgccatcgta aatcctgtaa ccggtgaagc ttaccaatat
       61 ggcgacccgg ttccaagtga atggcgcgac ccgtcattta ttgacccacg cgatccgcga
      121 tcatacggga caccacctga taatccagca agatattacg aacaacggca tattttggcc
      181 ggtttgagct ggaaatttta aacaattaat tcaaattatt gattagtttt gataacgctt
      241 tcaggcggat tattaaattt ataataggca ttatatcaat aattgaatta caatgaaaaa
      301 aataattact cctctcctgt ttagcctttt ttatttattc tccaataatc taactgcaca
      361 attatgcaac gatattccta tttcactggt tggtgcacca ttcaaccttg tagcaagtga
      421 tgttatcagc tatggcgaca gcatgttaac ttttgaactc aaaaatcaac atcccaccca
      481 attttttgct tatcctcaag cgaaattagt tgcattagca ccattaccac ccggcatgtc
      541 gttagctacg gtaaatttag actggatggt atttgcttct gcttggaatg tcgactctgt
      601 tgctcttgtc cgcatttatt acaatgttga agagcctatt acggaaaact ttaccgttcc
      661 gatgcaaata tgggtaaaca acttatcacc agttaccgac tcatgctatt tttcaaaaaa
      721 ttatgttgtg aatctaaatc ccactcctat ggctgtaaat acattggaat cacaaacatt
      781 acatattttt ccaaacccga taacagcaaa tcagcctatt tctattcaaa ataatcaatc
      841 gcttaactta cagcaattta ccattactaa tctggttggt gaactcataa aaagcggaac
      901 aattcaaaat aatacgattc caacaactga tttgaacaaa ggattttatt tgttaactat
      961 tttggttgat aatggattta aacaaacatt taaaatactt attgattaaa tgaaatttac
     1021 atttttagca gtaattgtac ttactttttg tgcagcatgg atatcgcccc aatctgaagc
     1081 acatttgatt tatcacgaca ataatgtgca acttaaatgg aaagactatc agggcacagt
     1141 agaagaagat gctaatatta cggcactgac atccataggt ttcagaatgg gatataaagg
     1201 cactgcacgc caagattcat taattgttgt tgtagaagct tttttcgaca aacaaaattc
     1261 atgggtgaaa aaggacacta ctgcttatgc cttaaaacat gaacaacttc attttgatat
     1321 tacagaatta tacgcgcgca aattaaagca atcaattcag agtgcttcgc ttacttacaa
     1381 aaattaccaa tcggtaatgc gtgatttaga aagaaaagca cataatgata tgaatactgc
     1441 agatagcatt tatgaggtgg caaccgatta tagcaagaat aaagtggaac aaaagaagtg
     1501 gaatgataac attgccgttg acctttccaa cctcaaggct tttgaagcag ataccttcaa
     1561 agtgcctgtg cggttctagc catcacttac atctaaacaa atcttactca ggcacttaag
     1621 ctaagttaat ggtggttaaa tttgattagc gacttttctt aacctaatta accacactat
     1681 gagtaagttc cccaagactt tgctcatccc ccttttggta ttcatgcatg gctactgcct
     1741 tgcccagacc ggtgatgtct attacatgcg tgccgattac gatgaaccct ggtgttgcgg
     1801 ttgggcgcct tacgaatttt caaatccatt aaatcttaca gcggtatttg gcccgggtgg
     1861 atggagttta aaatactatg aatacacaga cgccaatgcc atttttaatg ataacacctg
     1921 ttatgttttt atggaaggcg gagatggcca tgctgatatg atggagtggt ttttttcgtg
     1981 taatcaaaca ctaatagaag attgggttta taatggtggc agtttgttca tcaatgctgc
     2041 acctaatgtt ggagatggat taagttttgg attcggcgat gtttggttat ggtatcctt
//
LOCUS       DAORKW010000613         2098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3315_length_2098_cov_2.756241, whole genome shotgun sequence.
ACCESSION   DAORKW010000613 DAORKW010000000
VERSION     DAORKW010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3315_length_2098_cov_2.756241"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..>2098
                     /locus_tag="PLB46_16115"
     CDS             192..>2098
                     /locus_tag="PLB46_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRF78104.1"
                     /translation="MSSAGKNIHKLSAAGVLVTLGIIFGDIGTSPIYVLSAVVGDHTI
                     SRELVLGGLSCVFWTLFIVTTFKYVYLALNNDNRGEGGIFALYAIHRRFKVKWAIIPA
                     LIGCAALIADGFITPPISISSAIEGLKLLNPNIDSVVPYVIIAMTVLFVIQQFGTNVV
                     GRAFGPIMGVWFVMIGLLGALQVIQNPDVLNALNPYWAIHLLTNYEGGFWLLGGVFLC
                     TTGAEAMYSDLGHCGKHNIRFSWTFVLVMLLLNYAGQSSYLLTQDGHTLDEHSPFYMI
                     MPEWFLPVGIIIATLATLIASQAIITGCFTLVNEAMKLKLWTNFKVIYPSTVRGQIYI
                     PAINWFLFVGCIAVVLLFREAKNMEAAYGLAITFDMIMTTILLASLMRLQKRNIILIG
                     LFAVVILAIEGSFLISNLNKFTHGGWFTVSLAVAIFFGLWIFYEARKLRAKHTEFVEI
                     NKYLPAIQDLMADETLPKESTHLVYMSMVNDKNHIDSNIIYSIFRKRPKRADVYWFVH
                     VDIANDPYLASYSVDTIIPKKMFFVRLRFGFKVEHKVNVMFRQIVEEMVQTGEVDIVS
                     NYPSLRKHGMYADFKFILLNSRVASDNKLTPWEQIVVKGYRFIKSLSLPTHEDFGLEL
                     TTVEYALVPISV"
BASE COUNT          624 a          327 c          435 g          712 t
ORIGIN      
        1 ataaaaatgc cgataaaatg aaattgttct tgttgtatta tggcgaaggt cattacaaaa
       61 caaaagatta tatcactttt gaaaaagaat acaatcaaaa gcagctcacc gattaagtgc
      121 ataaaatggt gcataagatt ttaggttttc tttcctttct aaaaaggtga ggggtatatt
      181 tgccgggttt tatgtcatct gctggaaaaa atattcataa actttctgct gccggagtgc
      241 tagtaacact cggaattatt tttggtgata ttggtacatc acctatttat gtattaagcg
      301 cagtagtagg cgatcacacc attagccgcg aacttgtttt gggcggattg agttgcgtgt
      361 tttggacttt gtttattgtt acaacgttca aatacgttta tctcgcttta aataatgata
      421 acaggggaga aggtgggatt tttgcgttat acgctattca ccgcaggttt aaagtaaaat
      481 gggcaattat tccggcttta attggatgtg cagcgcttat agcagacggt tttataacac
      541 caccaatttc aatttcttcg gcaattgagg gtttaaaact cctgaatcca aatattgaca
      601 gtgttgttcc ttatgttatt attgccatga ccgttctgtt tgtcattcag caatttggaa
      661 cgaacgttgt tggtagggca tttggcccaa ttatgggtgt ttggtttgtc atgattggtc
      721 tgttaggagc attgcaggta attcaaaacc ctgatgtatt aaatgctttg aatccttatt
      781 gggctattca tttactcact aattatgaag gtggattttg gcttttaggt ggtgtattcc
      841 tttgcaccac gggagcagaa gcaatgtata gtgatttagg acattgcggt aaacataata
      901 ttcgattcag ttggacattt gtactggtga tgttattact taactacgcc ggccaatcgt
      961 catatctgct tacacaagac ggacataccc tcgacgaaca tagtccgttt tatatgatta
     1021 tgcctgaatg gtttttacct gtaggaatta tcatagccac attagctact ttaattgcaa
     1081 gtcaggccat tataaccggt tgttttacct tggtaaatga agccatgaag ttaaaactct
     1141 ggaccaattt taaagtaatt tatccatcta cagtaagagg tcaaatatat ataccagcta
     1201 taaactggtt tttatttgtg ggatgtattg cagtggtgtt gttatttaga gaagctaaaa
     1261 atatggaagc cgcctatgga ttagccatta catttgatat gataatgaca accattttat
     1321 tagccagttt aatgcgttta caaaaacgta atatcatatt aataggttta tttgctgttg
     1381 taattctggc tattgaaggt tcattcctga tttccaattt gaataaattc acacatggtg
     1441 gttggtttac agtatcatta gcggtggcga tattttttgg attatggata ttttatgaag
     1501 caagaaaact acgcgcaaaa catactgagt ttgtcgaaat caataaatat ttgcctgcta
     1561 ttcaggattt gatggcagat gaaacattac caaaagaatc tacccatttg gtgtatatgt
     1621 cgatggttaa tgataaaaac catattgact ctaatattat ttattccata ttccggaaac
     1681 gaccgaaacg agctgatgta tattggtttg tgcatgttga tattgccaac gacccttatt
     1741 tagcgagtta ttcggtagat acgattattc cgaaaaaaat gttttttgtg cgcctgcgat
     1801 ttggatttaa ggttgaacat aaggtgaatg tgatgttcag acaaatagtg gaagaaatgg
     1861 tgcaaacagg tgaagtggat attgtaagta attatccaag tttgagaaaa cacggtatgt
     1921 atgccgactt caaatttatt cttcttaact cgagagttgc aagtgataat aaactaactc
     1981 cttgggaaca aatagtagtg aagggttatc gcttcatcaa atcgttgagt ttacctacac
     2041 atgaggattt cggattagaa ctcaccaccg ttgaatacgc gctggttcca atcagtgt
//
LOCUS       DAORKW010000614         2097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3318_length_2097_cov_3.594025, whole genome shotgun sequence.
ACCESSION   DAORKW010000614 DAORKW010000000
VERSION     DAORKW010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3318_length_2097_cov_3.594025"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..>2097)
                     /gene="infB"
                     /locus_tag="PLB46_16120"
     CDS             complement(10..>2097)
                     /gene="infB"
                     /locus_tag="PLB46_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRF78105.1"
                     /translation="KRPHQHGGGADDPNKKKRKRIKKHERINVDKVKEEDAHRENRSR
                     NPIGVNLKKPAIVDPEAAQRQIQEQIRNTLAKLSGNNQQRGKGARVAKQLKREKREAA
                     EIEAAEALTSNVLQVAEFTSLSELASLMNASPTDLVQSCFNLGMMVSINQRLDAEIIE
                     LLAEENGFKVQFINVNEDDIEEEEEADEPDTLVTRPPIVTIMGHVDHGKTSLLDYIRE
                     TNVVAGEKGGITQHIGAYEVDLPNGKRIAFLDTPGHEAFTAMRARGAKLTDIAIIVIA
                     ADDQVMPQTKEAISHAQAANVPMVFAINKIDKPGANVDKIKEQLSQMNILVEDWGGKF
                     QSQEISAKSGIGVETLLEKLLLESELLDLKANPDKPAVGSTIEASLDKGRGYVCNIMV
                     QEGTLHVGDMMVAGPYYGKVKAMFDERNQRVNDAGPSTPVLVLGLNGAPQAGEKFKIY
                     EDEAEAKAVANKRSQILREQGIRTKKHITLEEIGRRKALGTFRELNIIIKADVDGSTE
                     ALTDSLQKLTTGEVQVNVIFKGVGQITESDVMLASASDAVIVGFQVRPSSSVRKLAEK
                     EGVEIRLYSIIYNAIEEIKAAMEGMLAPKMEEKVTCTVEVREAFKISKVGTVAGCYVS
                     EGKITRNTKIRVLRDGIVIHTGTLSSLKRFKDDAKEVTTSMECGLTIHNFNDIEVGDI
                     LDGYEEVEVTRTL"
BASE COUNT          507 a          503 c          395 g          692 t
ORIGIN      
        1 ggtaattatt tacagtgttc gtgttacttc cacctcttca tatccgtcca gaatatcacc
       61 aacttctatg tcgttaaagt tatggatggt taaaccgcat tccatgctgg tagtaacctc
      121 tttcgcatca tctttaaagc gtttgagtga gctcaacgtt ccggtatgaa ttacgatgcc
      181 atcgcgtagt acgcggattt tagtgttacg ggtaatttta ccttccgaca cataacatcc
      241 ggcaacggtt cccactttcg aaattttgaa tgcttcacgc acttcaacgg tgcaggtaac
      301 tttttcttcc attttaggag caagcatacc ttccattgcc gccttgattt cttcaatggc
      361 attgtagata atgctataca acctgatttc aacgccttct ttctctgcca atttacgcac
      421 agagctgctt ggacgaacct ggaaaccaac tattactgca tctgaagcac tcgctaacat
      481 tacatcgctc tcggtaattt gaccaacacc tttgaaaatt acatttacct gaacttctcc
      541 ggtagttaat ttttgtaagc tgtcggttaa tgcttctgtt gaaccatcca cgtcggcttt
      601 gattatgata tttaattcgc ggaaagtacc caaagctttt ctgcggccaa tttcttcgag
      661 ggtaatatgt tttttagtac gaataccttg ttcacgtaaa atctgactgc gtttgttcgc
      721 aacagctttt gcttcggctt cgtcttcgta aattttgaat ttttcacccg cttgcggcgc
      781 tccgtttaat ccgagcacta ataccggtgt agaaggaccg gcatcgttta cacgctgatt
      841 acgttcatca aacatcgctt tcaccttgcc ataataagga ccggcaacca tcatatcacc
      901 cacatgtaat gttccttcct gaaccatgat attacaaaca tacccgcgtc ctttatccaa
      961 tgatgcttca atggttgaac caactgcagg tttgtccgga tttgctttta aatccagtaa
     1021 ttccgattct aataataatt tttcaagcag tgtttctaca ccgatacccg attttgctga
     1081 aatttcctga ctttggaatt ttccacccca gtcttccact aaaatattca tttgcgaaag
     1141 ctgttcttta attttatcaa cgttggcacc cggtttatca attttattga tagcaaatac
     1201 cataggcaca tttgcagcct gcgcgtgact aatcgcctct tttgtttgag gcatcacctg
     1261 atcgtcggca gctattacga tgatggcaat atcggtaagt ttggcaccac gggcacgcat
     1321 ggcggtaaac gcttcgtgtc ccggtgtatc taaaaatgca atacgttttc cgtttggtaa
     1381 atctacctcg taggcaccaa tgtgctgagt gataccacct ttttctcccg caaccacatt
     1441 tgtttcacga atataatcga gcagcgatgt tttaccgtgg tcaacgtgac ccataatggt
     1501 aacaatcggc ggacgtgtaa ctaaagtatc aggctcatca gcttcctctt cttcttcaat
     1561 atcatcttcg ttaacgttga tgaactgtac tttaaagcca ttttcctctg ctagtaattc
     1621 aataatttcg gcatctaaac gctggttgat agacaccatc ataccaaggt taaaacagct
     1681 ttgcaccaaa tcggtagggg aggcattcat caaacttgcc aactccgata atgatgtaaa
     1741 ttctgccacc tgcaatacat tgctggtaag tgcttctgct gcttcaattt cagctgcttc
     1801 gcgtttttcg cgtttcaact gtttggccac acgggcacct tttccacgct gctgattgtt
     1861 tccactcagt ttagcgaggg tattgcggat ttgttcctga atttgacgtt gtgcagcttc
     1921 cgggtcaaca atagcaggct ttttgaggtt aaccccaata ggatttctgc tgcggttttc
     1981 gcggtgagca tcttcttctt taaccttatc tacattaata cgctcatgct ttttaatgcg
     2041 tttgcgtttt ttcttgttag gatcatcggc accaccaccg tgttgatgag ggcgttt
//
LOCUS       DAORKW010000615         2095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3324_length_2095_cov_3.302941, whole genome shotgun sequence.
ACCESSION   DAORKW010000615 DAORKW010000000
VERSION     DAORKW010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3324_length_2095_cov_3.302941"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /gene="glgB"
                     /locus_tag="PLB46_16125"
     CDS             complement(<1..1072)
                     /gene="glgB"
                     /locus_tag="PLB46_16125"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HRF78106.1"
                     /translation="MESTLKYDYSKFTDYDIHLFREGKHFKLWEKFGAFVLDEPDHTG
                     VYFAVWAPNAKTVHVTGTFNNWDKQQLPLKVRDDGSGIWEGVVTSALPGDLYKFIILG
                     ANNEIYEKADPYARRSEPAPGTASVIWDNSYTWNDNVWMVKRKPFGFNKPMSTYEVHL
                     GSWGRQPDNPEGFLSYRELAETLVPYVKEMGFTHVELMPVSEHPFYGSWGYQTTGYFA
                     PTSRYGTPQDLMYLIECFHKEHIGVLLDWVPSHFPDDGHGLIFFDGTHLYEHEDKRKG
                     FHPDWNSYIFNYGRFEVKSFLISSALYWLECFHIDGLRVDAVASMLYLDYSRKEGEWL
                     PNIYGGNENLEAIQFLKELNERL"
     gene            1255..>2095
                     /locus_tag="PLB46_16130"
     CDS             1255..>2095
                     /locus_tag="PLB46_16130"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRF78107.1"
                     /translation="MTKKLSILILVLLTYSGVRAQSNSLLYEISGNGLTAPSYIYGTM
                     HVSDRIAFHLTDSFFIALKSADVIALESNPETWIENDFGNPDKSPNNNYEYDEMDYAY
                     EYNQSDFYTTATKIEFPDANMYAGMFVDYSWLINGYLYRYDAQRTDYEEETYLDLFIF
                     QCGKKLAKEVAALEGEQEVLKLLQKAYEPSKDEDEEKKYIEKKRMQEAFKEDGMSISE
                     QIDQAYRDGDLDKLDSLERVTSPSSQFHKYFIEERNVNMVNRLDSILRSGKVVFTGIG
                     AAHL"
BASE COUNT          646 a          414 c          425 g          610 t
ORIGIN      
        1 ataatctttc gttgagttct tttaaaaact gaatggcttc gaggttttcg tttcccccat
       61 aaatattggg cagccactct ccttctttgc gggaataatc gaggtataac atgctggcta
      121 ctgcatcaac ccgcaagcca tcgatatgga aacattcgag ccaatagagt gcactgctga
      181 ttaaaaaact ttttacttcg aaacgaccgt aattaaaaat ataactattc caatcggggt
      241 gaaacccttt gcgtttgtcc tcatgctcat acaggtgcgt gccgtcgaaa aatatcaatc
      301 catgcccatc atcagggaaa tgcgatggca cccaatcgag caaaaccccg atatgttctt
      361 tgtgaaaaca ttcaataaga tacatgaggt cctgaggtgt tccataacgg cttgtcggcg
      421 caaaataccc tgttgtttga tatccccatg atccgtaaaa cgggtgttcg ctaaccggca
      481 tcagttccac atgggtgaac cccatttcct ttacataagg cacaagggtt tctgccagtt
      541 cgcggtaact caaaaagcct tcaggattat cgggttgtcg tccccaggat cccaaatgca
      601 cttcataggt gctcatgggt ttattaaatc caaagggttt gcgttttacc atccacacat
      661 tgtcgttcca ggtgtagctg ttatcccaaa ttactgaagc agtccctgga gccggttctg
      721 agcgtcgtgc ataggggtcc gctttttcgt aaatttcgtt attggcacct aagatgataa
      781 atttatataa atcgcctgga agtgctgaag ttacaactcc ttcccaaata ccggagccat
      841 cgtcgcgcac cttgagtggc agttgttgtt tatcccaatt gttaaatgtg ccagttacat
      901 gtactgtttt ggcattaggt gcccaaactg caaaatacac accggtatga tcaggttcat
      961 ctaatacaaa agcaccaaat ttttcccaga gcttaaagtg tttgccttcg cggaacagat
     1021 gaatgtcata atcggtaaac tttgaatagt cgtatttaag cgtggactcc atactgcaaa
     1081 tttagttagg gatatgattt ataatgaacc tgcactcata aaatcatcaa catcctaaag
     1141 tgaacagcac cgagccggtt aatttatgac atttatttgt agctacaggt ataccaaatt
     1201 ggggttaaaa tgcgtattta tgtttaacat tgcaacctag gtaaaacacc aaacatgacc
     1261 aaaaaactta gtatcctcat tctggtactg cttacttaca gcggcgttcg tgcacaatca
     1321 aattcgttac tctatgaaat cagtggtaac ggtttaacag cgccttccta tatatacggc
     1381 accatgcatg tgagcgatag aattgctttt catttaaccg atagtttttt tattgcatta
     1441 aaaagtgcag atgtaattgc attggaaagc aatccggaaa catggataga aaatgatttt
     1501 ggcaatcctg ataaatcacc gaacaacaat tacgaatacg atgagatgga ttatgcatac
     1561 gaatataatc agtcggattt ttacactaca gctactaaaa ttgaatttcc ggatgccaat
     1621 atgtatgcgg gtatgtttgt agattacagt tggttgataa acggatattt atatcgctac
     1681 gatgctcaac gcaccgatta tgaagaggaa acttatcttg atttatttat tttccagtgt
     1741 ggtaaaaaat tagctaaaga agtggcagca cttgagggag aacaagaagt attaaaactt
     1801 ttgcaaaaag cctatgaacc ttctaaagat gaagatgaag aaaaaaaata tatagaaaaa
     1861 aagcggatgc aagaagcgtt taaggaggat ggaatgagta ttagtgaaca aatagatcag
     1921 gcatatcgcg atggtgactt agacaaatta gattcattag aacgtgtaac gtctccctct
     1981 tcgcaatttc ataaatattt tattgaagaa cgcaatgtga atatggtaaa tcgcctcgat
     2041 tcaattttac gcagcggcaa agtggtgttt accggtattg gtgcagcgca tttgc
//
LOCUS       DAORKW010000616         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3329_length_2094_cov_4.799902, whole genome shotgun sequence.
ACCESSION   DAORKW010000616 DAORKW010000000
VERSION     DAORKW010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3329_length_2094_cov_4.799902"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1791)
                     /locus_tag="PLB46_16135"
     CDS             complement(<1..1791)
                     /locus_tag="PLB46_16135"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRF78108.1"
                     /translation="MKMFRKQLYVIFSTAMLTCLSSTTVLAGEQISVDRTLNWSAQTR
                     TFTTESQFSGESFHFDGAAFNFNNTTVLPEYGESFSITSTASIQVRIENAVYEPVTTI
                     GGKLIESIKPQLSNQIDLKYQVGFAQKAPQLDVYFVPMRVNASTGKIEKLIQFTLVIT
                     TSGNISYKTTDEDEFGDGSVLATGSWYKVSVVREGIFKIDRNWLNSNGISGTVSFDNF
                     GVFGNGGGMLPESNAAFRYDDLQENAIYKKDVNGNGLFDDGDYIYFFGQAPHRWNYNE
                     ATNKFTHANNLYSDQTYYFITPDAGSGKYMTDKASEATENISTSSYDYLTFFDTDKIN
                     LLNSGRKWYAEVIDIYNPNRSYNFVMPGLITSEIVNIRASAAGSSLTGSTILKMDANG
                     ISLPTNIIVSELVEGFNETFAYESAIETGFTSTSENITIGVEFSGASVNTTWMNYIEL
                     LGRANLKHNNAQLYFRDSRTRADGNITKYILDADNDIKIWDVTNQINVQNQLFVEEGG
                     DIIFRASSDTLRNFIAFTDADVFSSSSIIFVEKIANQNIHGITTQPDYVIVTHPAFLS
                     EARRLAGFHHDTQGLDTMVVSIYDVYNEFSS"
     gene            1824..>2094
                     /locus_tag="PLB46_16140"
     CDS             1824..>2094
                     /locus_tag="PLB46_16140"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF78109.1"
                     /translation="MPLKRKYHKNIAIIPSFSGLIPLWVITTCFTILFSIESQQVQAQ
                     DLKYGHWHAAETNYNPAFAGSSGQPRIILNFRDQWPVMPQAYVSYR"
BASE COUNT          652 a          394 c          348 g          700 t
ORIGIN      
        1 tgatgaaaat tcgttgtaaa catcataaat actcactacc atagtatcaa gaccttgtgt
       61 gtcatgatga aatcctgcta aacgacgtgc ttctgaaaga aatgctggat gtgttacaat
      121 aacataatca ggttgagtgg taataccatg aatattttga ttggcaattt tttctacaaa
      181 tattatcgat gaacttgaaa aaacatcagc atcggtaaag gcgataaaat tgcgcaatgt
      241 atcagatgaa gcacggaaaa taatatctcc gccttcttca acaaataatt gattttgaac
      301 atttatttgg tttgttacat cccaaatttt aatatcatta tctgcatcta aaatatattt
      361 cgtgatattg ccatcagcac gagttctgct atctctaaaa tataattgtg cattattgtg
      421 ttttaaattt gctctgccta ataattcgat ataattcatc catgttgtat tcaccgatgc
      481 gcctgaaaat tcaacgccaa ttgttatatt ttctgatgta gatgtaaaac ctgtttcaat
      541 agcactttcg taagcaaatg tttcattaaa cccttccact aattctgata caataatatt
      601 agttggcaag ctgattccat tggcatccat ttttagaata gtgcttcccg taaggcttga
      661 acctgcggcg cttgctcgga tatttacaat ttctgatgtt attaaaccgg gcataacaaa
      721 attgtatgaa cgattagggt tatatatatc aattacttcg gcataccact ttcttcctga
      781 atttaataaa ttgattttat cggtgtcaaa aaaagttaaa taatcgtagg aggatgtact
      841 gatattttcg gtggcttcag acgctttgtc ggtcatatat ttaccacttc ctgcatccgg
      901 cgtaataaaa taataagttt ggtcgctata caaattattg gcatgcgtaa atttatttgt
      961 agcttcatta taattccagc gatgtggcgc ttggccaaag aaataaatat aatcgccgtc
     1021 atcaaataaa ccattgccat ttacatcttt tttataaata gcattttctt gtaaatcatc
     1081 atatcggaat gcggcattac tttccggtaa catgccacca ccattgccaa atacaccaaa
     1141 attgtcgaat gaaactgttc cgctaatacc attactgttt aaccaattac ggtctatttt
     1201 aaatattcct tctctaacaa cactcacttt ataccatgaa cctgttgcta ataccgaacc
     1261 atcgccaaat tcatcttcat ctgttgtttt ataactaata tttccggagg tggtaatgac
     1321 taaagtaaat tgaattaatt tttcaatttt accggttgaa gcatttacac gcattggaac
     1381 aaaatacaca tctaattgcg gtgctttttg tgcaaaacca acttgatatt ttaaatctat
     1441 ttgattgctc aattgtggct taatactttc aattaattta ccgccaatag ttgtaacagg
     1501 ttcataaact gcattttcta tgcgcacctg aattgatgcc gtgctggtta tggaaaacga
     1561 ctctccgtat tcaggtaaaa cggtggtatt attaaaatta aaagctgctc catcaaaatg
     1621 aaatgattca ccgctaaatt gactttcggt agtaaatgtt cgtgtttgtg ctgaccaatt
     1681 gagtgttcga tccaccgaaa tttgttcacc tgccaatact gtagtcgacg ataaacacgt
     1741 taacattgca gttgaaaaaa taacatagag ttgtttgcga aacattttca tcgctgattt
     1801 aggatttgaa actaaggtta aaattgccac ttaaacgcaa atatcataag aatattgcca
     1861 ttattccgtc attttcggga ttaataccat tatgggtgat aaccacctgt tttacgattt
     1921 tgttcagtat cgaatcgcaa caggttcagg ctcaggattt aaaatatggg cactggcatg
     1981 ctgcagaaac aaattacaac cctgcatttg caggttcatc agggcaaccg cgtatcatcc
     2041 tcaatttccg cgaccaatgg ccggttatgc cgcaggcgta tgtgtcgtac agag
//
LOCUS       DAORKW010000617         2093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3335_length_2093_cov_4.182532, whole genome shotgun sequence.
ACCESSION   DAORKW010000617 DAORKW010000000
VERSION     DAORKW010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3335_length_2093_cov_4.182532"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PLB46_16145"
     CDS             <1..424
                     /locus_tag="PLB46_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRF78110.1"
                     /translation="SVMVFPVVVQIVYEVLKIVPVTLREATLSLGATKWETIKKVVIK
                     KASPGILAACILGFSRAFGETIAVLMVVGNVVQVPKGIFDEGYPIPSLIANNYGEMMS
                     IPMYDSALMFAALILLLIILVFNIYARWVLNRMEKKIA"
     gene            427..1275
                     /gene="pstA"
                     /locus_tag="PLB46_16150"
     CDS             427..1275
                     /gene="pstA"
                     /locus_tag="PLB46_16150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRF78111.1"
                     /translation="MNKRKLEEKIFNIIMRIAAFIIFGSLIAISITILIEGIPSMNMD
                     MITKTPQGGFYLGKEGGILNAIAGSFYLAIGATLFALIICLPTVIYINIYAPKNSKFA
                     NLTRLCLDVLYGIPSIVFGAFGFVLMLLIGMKVSLLAGIITVGLLIVPIIARTMDEVI
                     RQVPGELQEAAFSLGATRWETAIKVVLKQGLPGLATAALLGFGRAIGDAASVMFTTGF
                     TDNIPHSINDPAATLPLAIFFQLSSPIAEVRERAYAAALILTVIIFAISLLSRLLIKR
                     FSKNTI"
     gene            1289..2056
                     /gene="pstB"
                     /locus_tag="PLB46_16155"
     CDS             1289..2056
                     /gene="pstB"
                     /locus_tag="PLB46_16155"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRF78112.1"
                     /translation="MTTTVKSHISIRNLNMMYGSNHALKNINIDIPDKKLTAIIGPSG
                     CGKTTLLKTFNRLIEGVDGTKITGEVLVDGENIYAKTADVTNIRKKMGLLNQRPTPLP
                     MSIFDNIAFGMRIHGEKNKRLLQDRVQHYLQVASLWDEVKDRLHTPAARLSIGQQQRL
                     CLARGLAVEPEIILGDEPTSALDPISTKHIENKFSELKEQYTIVMVTHILRQARRIAD
                     YVIFMYLGEIIEHGPTEQVLTNPQHQSTKDYVGGTIS"
BASE COUNT          686 a          341 c          408 g          658 t
ORIGIN      
        1 atcggtaatg gtttttcccg ttgttgttca aattgtatat gaagtactta aaattgtacc
       61 agttacattg cgagaagcaa cgctttcact tggtgctaca aaatgggaaa caattaaaaa
      121 agtagtaatt aaaaaagcct cacctggtat attggctgct tgtattttag ggttttctcg
      181 tgcatttggt gaaacaatag ctgttttaat ggtggttggg aatgttgtac aggtaccgaa
      241 aggaattttc gatgagggtt atccaattcc atcattaata gcaaacaatt acggtgaaat
      301 gatgagcata cccatgtatg attctgcgct catgtttgca gcattaattt tattattaat
      361 cattctggtt tttaatattt atgcccgttg ggtgttgaac agaatggaga aaaaaatcgc
      421 atgattatga ataagagaaa actggaagag aaaatattta acatcataat gcgcatagca
      481 gctttcatca tatttggaag cctcattgct atttcaatta ctattcttat tgagggtatt
      541 ccttccatga atatggatat gattactaaa actccgcaag gtggtttcta cctgggtaaa
      601 gaaggtggga ttttaaatgc aattgcggga tcgttttatc ttgcaattgg tgctacgcta
      661 tttgcattaa ttatttgttt acctactgta atttatatca acatttatgc acctaagaat
      721 tccaaatttg ccaatcttac acgtttatgt ttagatgtat tatacggcat tcctagtatt
      781 gtatttggtg catttggatt tgtactaatg ttgctgattg gtatgaaagt aagtttgtta
      841 gcaggtatta ttaccgttgg gttattaata gtacccatta tagcccgaac catggatgaa
      901 gttataagac aagttccggg agagttgcag gaagccgcat ttagtttagg cgcaacacgc
      961 tgggaaaccg caataaaagt tgttttaaaa cagggattac ccggattagc aactgcggca
     1021 ttacttggat ttggacgtgc aattggagac gccgcatcgg taatgttcac aacagggttt
     1081 accgacaata ttccacattc aataaatgac cctgcagcaa ctttaccatt agcaatattc
     1141 tttcaattaa gttcaccaat tgctgaagtg agagaaagag cttatgctgc agcattaata
     1201 ttaactgtaa tcattttcgc aataagttta cttagccgac ttttaattaa aagattttct
     1261 aaaaatacca tctgaaataa attattttat gacaacaact gtaaaatcgc atatcagtat
     1321 ccgcaatctc aatatgatgt acggatctaa ccatgcttta aaaaatataa atattgatat
     1381 ccctgataaa aaattaaccg ccataattgg ccctagtgga tgcggtaaaa ctaccttact
     1441 taaaacattt aatcgtttga tagaaggagt agatggcacc aaaattactg gtgaagtatt
     1501 ggttgatgga gaaaatattt atgccaaaac tgctgatgta acaaatattc gtaagaaaat
     1561 gggtttgcta aatcaacgac ctacaccatt gccaatgagt attttcgata acatcgcatt
     1621 tggtatgcgt attcatggtg aaaaaaataa acgcttgttg caagatcgcg tacaacatta
     1681 tttacaagtt gcgagtttgt gggatgaagt aaaggacagg ttacatacac cggcagcaag
     1741 attgtctatt ggccagcaac aacgtttatg tttagcaaga ggccttgctg ttgaacctga
     1801 aattatctta ggagatgaac ctacaagtgc tttagaccca atttcaacta aacatattga
     1861 aaataaattt tcagaattaa aagagcaata caccattgta atggtgacac atattttacg
     1921 ccaggcaaga agaattgctg attatgtgat tttcatgtat ttaggcgaaa taatagaaca
     1981 tggtccgaca gagcaggttt taaccaaccc gcaacaccaa tcaacaaaag actatgttgg
     2041 aggaacgata agctgacaca cagtttttca taaatcggtt aggttgagaa ccc
//
LOCUS       DAORKW010000618         2089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3348_length_2089_cov_4.157325, whole genome shotgun sequence.
ACCESSION   DAORKW010000618 DAORKW010000000
VERSION     DAORKW010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3348_length_2089_cov_4.157325"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLB46_16160"
     CDS             <1..730
                     /locus_tag="PLB46_16160"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRF78113.1"
                     /translation="FSFEKINTGDNRPRLQPQEEQQLLYLSVYIPMVFVILMWIVKLF
                     EVVTEIDLGVFGILPQHVIGLRGILFSPFLHADWEHLLSNTVPFFILSFLMIFNYRKV
                     AYKSFLFIYLFGGLLLWITGRENYHIGASGLIYGMAFFIFFSGVFRKNIQSIALSLFV
                     VFLYGSIVWGVLPVDLTISWEGHLMGAICGGISAFYFRNVDLPAPVILEDEPDDELSE
                     EALSANKEKIHIHYEIIDKVTDKP"
     gene            727..1395
                     /gene="mnmD"
                     /locus_tag="PLB46_16165"
     CDS             727..1395
                     /gene="mnmD"
                     /locus_tag="PLB46_16165"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HRF78114.1"
                     /translation="MNREVIITGDGSPTLFVPELNEHYHSIHGALNESLHVFIQNGLI
                     HQLTIHQHIHLLEIGLGTASNLLLTINAMNNSPIKVHYEALEPFPIQPGIVAQLTDLY
                     AQQFSTSATLFQSIHQQPANIALQINPQFKFTRIEEKLENAILLPQYNLVYFDAFGPR
                     AQPEMWDKSNFEKIYAAMEQDGTLVTYCAKGEVRRNLQAVGFLVERLPGPPGKREMLR
                     AIKK"
     gene            1410..>2089
                     /locus_tag="PLB46_16170"
     CDS             1410..>2089
                     /locus_tag="PLB46_16170"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF78115.1"
                     /translation="MVDFLIVGGGIAGINVAWQLHQRGAGFHLIDTCQYNSATSIAAG
                     LINPVTGRKFSTQWNIDELIPLAEKTYSEIGSFLNSDFLFKKSIFKIHKNESVVEDWL
                     KIKSAQPVSAYINAFPDSTSYAKYLDFRFGAIGVYPAFHLNMVVLQQQFYAAFSNCIL
                     QEQLVYDTLKIEPNGINYKGNIYRNVIFCDGVAAQKNPWFNFISFKPAKGECLIIEVP
                     NMQLHEII"
BASE COUNT          644 a          355 c          379 g          711 t
ORIGIN      
        1 attttccttc gaaaaaatta atacaggcga taaccgacca cggttacaac cacaggaaga
       61 gcaacaattg ctttacctga gtgtttatat acctatggta tttgtaatcc ttatgtggat
      121 tgtcaaactc tttgaagtag ttaccgaaat cgatttaggt gtatttggca ttttaccaca
      181 acatgtaata ggattaaggg gaattttatt ctctcctttt ttacatgccg actgggagca
      241 tttactttct aatactgttc cattttttat tttatcattc ctgatgattt ttaactatcg
      301 gaaagtagct tataaatcgt ttttgtttat ttatttattt ggtggtttat tgttatggat
      361 aacaggtcgt gaaaattatc atattggtgc ttccggatta atttacggca tggcattttt
      421 tatatttttt agtggtgttt ttcgtaaaaa cattcaatct attgcacttt cattatttgt
      481 tgtgttttta tatggcagta ttgtctgggg agtgcttccg gttgatttaa ctatctcgtg
      541 ggaaggacat ttaatgggtg ctatttgcgg cggaataagt gcattttatt ttcgtaatgt
      601 ggatttacct gctccggtta ttttagaaga cgagccggac gatgagctta gtgaagaagc
      661 cctttcagca aataaagaaa agattcacat ccattacgaa attatagata aagtaaccga
      721 taaaccatga atcgcgaggt gataattacc ggtgacggct ctcctactct tttcgtaccc
      781 gaattaaatg aacattacca ttctattcat ggcgcgctaa atgaatcatt acatgtgttt
      841 attcaaaatg gattaattca tcaattaacc attcatcaac atattcattt attggaaatc
      901 ggattaggca ctgcaagtaa tttattgctg acgattaatg caatgaataa ttcaccaatc
      961 aaagttcatt atgaagcact cgaaccgttc ccaattcaac ctggaatagt agcacaactt
     1021 acagatttgt atgcgcaaca attttcaaca tctgctacat tatttcaatc tatacatcag
     1081 caaccagcaa atattgccct ccaaataaat ccacaattta aatttactcg aattgaagaa
     1141 aaactggaaa atgcgatttt attaccccaa tacaatttag tgtatttcga tgcatttggc
     1201 cccagagcac aaccggaaat gtgggataaa agtaattttg aaaaaatata tgctgccatg
     1261 gaacaagatg gcactttagt tacttattgc gcgaaaggag aagtgcgcag aaatctacaa
     1321 gcagttgggt tcctggtaga acgcctccca ggccctccgg gaaaacgcga aatgctacgc
     1381 gctatcaaaa agtaatttaa ttttacatcg tggttgactt tcttatagta ggtggtggaa
     1441 ttgccggtat aaatgttgcc tggcaactac atcagcgtgg tgccggtttt catttaattg
     1501 atacttgtca gtataatagt gctacatcta ttgcagcagg tttaattaat cctgtaaccg
     1561 gcagaaaatt ttccacccaa tggaatatcg atgagttaat tcctttagct gaaaaaactt
     1621 attcggaaat tggtagtttt ttaaacagtg attttttgtt caaaaaaagt atttttaaaa
     1681 ttcataaaaa cgaatcggta gttgaagatt ggcttaaaat taaatctgca caacccgttt
     1741 ccgcttatat caatgccttt cctgattcaa catcgtatgc caaatatctc gatttcagat
     1801 ttggtgcaat tggtgtgtat cctgcctttc atttaaatat ggtggtattg cagcaacagt
     1861 tttatgcggc gttttcgaat tgtattttgc aagagcaatt agtatatgat accttaaaaa
     1921 ttgaaccaaa tggtattaat tacaaaggca atatttatcg caacgttatt ttttgtgatg
     1981 gcgtagccgc acaaaaaaat ccttggttta attttatatc atttaaacct gcaaagggcg
     2041 aatgtttaat aattgaagtg ccaaatatgc aattgcatga aatcatcat
//
LOCUS       DAORKW010000619         2078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3371_length_2078_cov_4.798814, whole genome shotgun sequence.
ACCESSION   DAORKW010000619 DAORKW010000000
VERSION     DAORKW010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3371_length_2078_cov_4.798814"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PLB46_16175"
     CDS             complement(<1..678)
                     /locus_tag="PLB46_16175"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HRF78116.1"
                     /translation="MPFNSNNSTITTTSIIIYSVIILAFSTIIHSCATDSEIINWYNA
                     DEYAAGGATTLYEATSAAFSTPAPNLSASAFQKHMEGDVTFEATFVTAPAPNNAGLGP
                     IFNDVSCVNCHTLDGRGDKPSVFRLSIPGTDDQGGPNPAPGFGGQLQDKAIFGTLAEG
                     NVTTTYIEEFYSYPDGTPYSLLRPIYTINNTYIPLPPGTMLSVRAAPPVFGLGLLEAI
                     NSFDIIAT"
     gene            complement(668..1735)
                     /locus_tag="PLB46_16180"
     CDS             complement(668..1735)
                     /locus_tag="PLB46_16180"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HRF78117.1"
                     /translation="MHTSQKIKLTSLVFITALFGFSSCKDDNPTDINNYDFQPILGHL
                     SQNVITETYIDLHAKSELLVDAVTTLQTDATPSNLEAARQAWRNTRKPWEQSEGFLFG
                     PVSTNGIDPSIDSWPVNVVDLEAVLSSGAVLTKSYIDGLEGTLKGFHTIEFLLWGENG
                     DKTIDAFTPREFEYLIATSQSLESDTYSLQNSWNPTGENFQYEIAMAGESGSMYISQK
                     SAMQEIINGMIAIADEVANGKIYDPLSQLDITLEESRFSANSKEDFKDNIRSIQNLYE
                     GKYLMDGSGISDFIISKDADLDAHIKAEIQASIDLIDAIPGTFTQAIFDNPAAIEAAQ
                     ESVRHLQEELESEVKPLIDAI"
     gene            complement(1755..>2078)
                     /locus_tag="PLB46_16185"
     CDS             complement(1755..>2078)
                     /locus_tag="PLB46_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78118.1"
                     /translation="NLNVKRTPIASAVLGYFAEAGYNVLSFLEKDVHRLDVFAGYYYY
                     DSMYKTSGEIFDNPRWERSEIRTGLNYCWNKNIAIKSDYTFRTIGIPDLNKENTFTLA
                     LAYHF"
BASE COUNT          638 a          391 c          380 g          669 t
ORIGIN      
        1 ggtagctatg atatcaaaac tgtttatggc ttctaacaac ccaagtccaa aaaccggcgg
       61 agctgcccga actgataaca tggttccggg agggagcggt atataggtat tattaattgt
      121 atatattggt cgcagcaaac tataaggagt gccatcagga taactataaa attcttctat
      181 ataagtagta gttacatttc cctcagcaag agtaccgaaa atggctttat cctgtaattg
      241 acctccgaaa ccgggagccg gatttggacc accttgatca tcggtacccg gaatacttaa
      301 tctgaataca ctgggtttat cgccgcgtcc gtcgagcgta tggcaattta cgcaactcac
      361 atcattaaat atggggccaa gacctgcatt attgggagca ggtgcagtaa caaatgttgc
      421 ttcaaatgtg acatcacctt ccatgtgttt ttgaaacgca cttgcactca aattgggagc
      481 aggtgttgaa aatgcagcag aagttgcttc gtataaagtt gttgcaccac cggcggcata
      541 ttcatccgca ttataccaat tgattatttc cgaatcggtg gcacaactat gaataattgt
      601 agaaaaggca agtattatca ccgaatagat gataatactt gttgtagtaa ttgttgaatt
      661 gttgctatta aatggcatca attaaaggtt ttacttcact ttctaattct tcctgtaaat
      721 ggcgaacaga ttcctgagcc gcttcaatag ctgcaggatt gtcgaaaata gcctgcgtaa
      781 aagtacccgg aatagcgtca attaaatcga tacttgcctg aatttcagct ttaatatgtg
      841 catctaaatc agcatctttt gaaataataa aatcactaat tccactgcca tccatcaaat
      901 attttccttc atataaattt tggatacttc tgatattatc tttaaaatct tctttgctgt
      961 ttgcactaaa tctgctctct tctaatgtaa tatccaattg tgaaagcggg tcgtaaattt
     1021 ttccgtttgc cacttcatct gcaattgcaa tcatgccatt tatgatttcc tgcatagcag
     1081 atttttgact gatatacata ctgcctgatt cgcctgccat agcgatttca tattggaaat
     1141 tttcgcctgt tggattccag ctattttgca aactataagt atcgctttcg agactttgtg
     1201 atgtagcaat taaatattca aattcacgtg gcgtaaatgc atcaattgtt ttatctccat
     1261 tttcacccca taataaaaat tcaatggtgt ggaaaccttt taacgtacct tctaagccat
     1321 cgatatatga tttagttaat acagcaccac tgcttaatac agcttctaaa tcaaccacat
     1381 ttaccggcca gctatcaata gatgggtcga taccattggt ggaaacaggt ccgaataaaa
     1441 atccttcaga ctgctcccat ggcttgcggg tattgcgcca agcctgacgt gcagcttcaa
     1501 gatttgaagg agtggcatct gtttgtaagg tagttacagc atctactaat aattcacttt
     1561 ttgcatgtaa atcgatatac gtttcggtaa ttacattttg agataaatgc cctaaaattg
     1621 gttggaaatc ataattgtta atgtccgttg gattatcatc tttgcacgaa gaaaatccaa
     1681 acaaagctgt aataaatact agcgatgtta attttatttt ttgagaagta tgcatgaaat
     1741 atatatttta aattttaaaa atgataagct aatgcaagtg tgaatgtgtt ttctttattt
     1801 aaatcaggaa taccaatagt gcgaaaagta taatcggatt taatggcaat atttttattc
     1861 cagcaataat ttaatccggt gcgaatttct gaacgttccc atcttggatt atcgaatatt
     1921 tcgccggatg ttttatacat ggaatcgtaa taataataac cggcaaaaac atctagacga
     1981 tgtacgtctt tttcgagaaa ggataaaaca ttataaccgg cttcagcaaa ataaccaagt
     2041 actgcgctgg caattggtgt acgttttaca ttcagatt
//
LOCUS       DAORKW010000620         2075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3383_length_2075_cov_5.377723, whole genome shotgun sequence.
ACCESSION   DAORKW010000620 DAORKW010000000
VERSION     DAORKW010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3383_length_2075_cov_5.377723"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLB46_16190"
     CDS             <1..1131
                     /locus_tag="PLB46_16190"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HRF78119.1"
                     /translation="ELASPVILLQSDDQFKFGFDDLSGGVKNYYYTFEHCTQQWESSE
                     LSSYDYIEGFEENRIDDYSFSFATLQKYTHYSLTFPNNDVQFRLSGNYVIKVWEEDNR
                     DKPVIVKRFFVREEMVAINGDVYRPNLIPYRNEYQEINFTIDIRNLDIGAAFDEMKVS
                     LMQNGRFDNIKYDIKPRLISNDMLYYDIDDIVFPAGKEYRRFDTKTLKFQSDRIVKIE
                     KEAGSYQVYVNTDESKVYQKYFFEKDANGQYVIHADLANNVETEGDYAWVNFVLQYPY
                     MITSGDVYIFGELSNNSMTPLNKMTYDFDKQQYIGKLYLKQGYYNYAYAVRSIGTSFA
                     DFTYTEGNSFETENDYSLLVYQHDFSVGYDRMIGYYQMNSIK"
     gene            1230..1748
                     /locus_tag="PLB46_16195"
     CDS             1230..1748
                     /locus_tag="PLB46_16195"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF78120.1"
                     /translation="MKQLLLSFCRYNLWANKKIIDALQLLPNDIADAQVNNSFTSIRK
                     TIYHIWDAQVIWLSRMQGISLSTWPSAEYGDDFAGYDLYFIRQCEDFIQFVETRHESF
                     FESICYYRVLSGKEFQTRNGDIVLHCMNHSTYHRGQIITMLRNLEVVTLPSTDFIEYN
                     RSVQENYSGGNR"
BASE COUNT          718 a          315 c          391 g          651 t
ORIGIN      
        1 gaattagcat cccctgttat tttgttacag tcagacgatc agttcaagtt tggctttgat
       61 gatttatcag gaggagtaaa aaactattat tatacatttg aacattgcac acaacaatgg
      121 gaaagtagtg aattgagtag ttatgattat attgaaggat ttgaagagaa tagaattgac
      181 gattacagct tttcatttgc cacattacaa aagtacaccc actacagttt aaccttcccg
      241 aacaatgatg tgcagtttag actaagtggt aattatgtaa taaaggtatg ggaagaagac
      301 aatcgggata agcccgtaat tgtcaaacgt ttttttgtta gggaagaaat ggttgccatc
      361 aatggggatg tttaccgacc caatttaata ccctatcgta acgaatatca ggaaatcaac
      421 tttacaattg atattcgcaa tctggatatt ggagccgcat ttgatgaaat gaaggtcagt
      481 ttaatgcaaa atggccggtt tgataatatc aagtatgata ttaaaccccg tttgatatcc
      541 aacgacatgc tgtattacga tattgacgat atagtttttc ctgcggggaa agaatatcga
      601 cgttttgata ccaaaacgct taaatttcaa tctgaccgta tcgtcaaaat tgaaaaagaa
      661 gctggcagtt accaggtata tgtaaatact gatgaaagta aagtttatca aaagtatttt
      721 tttgagaaag atgcaaatgg tcagtatgta attcatgccg acctggcaaa caatgttgaa
      781 accgaaggtg attacgcctg ggttaatttt gttttacaat atccctacat gattacttcg
      841 ggtgatgtgt atatatttgg tgagttaagc aacaattcaa tgaccccact caacaaaatg
      901 acctatgatt tcgacaagca acaatatata ggcaaactgt acctaaagca aggttattac
      961 aattatgcat atgctgttcg ttcaattgga acttcatttg ccgatttcac ctataccgag
     1021 ggaaatagtt ttgaaactga aaacgactat tcactcctgg tataccaaca tgacttcagt
     1081 gttggttatg atagaatgat agggtactat cagatgaatt ctattaaatg agtttccctt
     1141 ttataagctg acccaattat tatccacaac aactcaattg taaattaggt gaaggaagag
     1201 tagtttagat gttcaatcat aactattgta tgaaacaact actgctttct ttctgcagat
     1261 ataatctttg ggcaaataaa aagataattg acgccttaca attattgccg aatgatattg
     1321 ctgacgcaca agtaaataat agtttcacca gcataagaaa aactatatat catatctggg
     1381 atgcgcaggt gatatggcta agccgtatgc agggtatctc gctcagcacg tggcccagcg
     1441 cagaatacgg cgatgacttt gccgggtatg atttatactt tattaggcag tgtgaagatt
     1501 ttattcagtt tgtagaaaca cgtcatgaaa gttttttcga atcgatttgt tattaccgag
     1561 ttttaagcgg gaaagaattt caaacaagga atggcgacat cgttttgcat tgtatgaatc
     1621 attccaccta tcatagagga caaattatta ccatgctacg taatttggaa gttgttacat
     1681 taccttctac tgattttatc gaatacaaca ggagtgttca ggaaaactat tcaggaggaa
     1741 acagataagc gcaagccgga ttattggttt attaataacc tggacaaaca taaaatgaat
     1801 atcgttttgg gttaaatgaa attgatttag cctatcacaa taagtaaatt tcaacaaaat
     1861 acaataccga atgaccaatc aaaaaagtaa atcaggaatt aaaaatggtt atttagggaa
     1921 taaatgaagc cgaaaattaa ttgttgaaag taggtttatc aacgaatatg tggtaaaaag
     1981 tacttattta taacattgtt gtatataata atgtgcaaaa catgtgaaaa tcaacaattt
     2041 tttttttaat tttttttgat aatataaatt tatgt
//
LOCUS       DAORKW010000621         2074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3390_length_2074_cov_4.730064, whole genome shotgun sequence.
ACCESSION   DAORKW010000621 DAORKW010000000
VERSION     DAORKW010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3390_length_2074_cov_4.730064"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1629
                     /locus_tag="PLB46_16200"
     CDS             148..1629
                     /locus_tag="PLB46_16200"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRF78121.1"
                     /translation="MNKQFKILLGPLLAVIVGYIFYATGNTPLVSKTAGVLILMAYWW
                     MTEAVNIYVTSLMPVVLFPLLGIMGIDTIAPQYMKDVIFLYIGGFMLTFAIERWDLHK
                     RFSLRILLKFGSSPEKLIFGFMAATFFISMWLNNTSTTLMMLPVALAVIKQIHGNDMT
                     KRSGLATAILLAISYASTIGGTATLVGTLPNMVMKKFYDENFQDAAPLDFANWFLFAF
                     PLSIVFLGCCFLVFKFILLPKSDHQLPDNSYCKTEYQKLGKMSFEEKAIAIIFSSAIV
                     LWFTLEDKDIWGLHLTGWKTILLNAGIIPDEKFVNESFVAILMGMSLFFFPSKNKKGE
                     TLISWEDVKRLPLGILFLFGGGFALAKGVEVSGLSLWIANNCAGLKVLDEWVMILALC
                     VITTIFSEFASNTATVSLFMAILIPIIPELGIPPLLVMMPVTIAASFSFMLPVGTPPN
                     TIVFGTELIQVKTMLRVGLWLDLIGAVVITAFTMTLGKLIFGI"
     gene            1816..>2074
                     /locus_tag="PLB46_16205"
     CDS             1816..>2074
                     /locus_tag="PLB46_16205"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF78122.1"
                     /translation="MKKVLLLLFVVSLTQLKAQLIINEVASTNYNITQDEEGDYPDWI
                     ELYNAGPTAVNLAGYAISDGKTLAPWVFKDSIIPANGRVLVY"
BASE COUNT          576 a          356 c          406 g          736 t
ORIGIN      
        1 ctgcatctaa ttggtcgatt actgacctta tcgtggactg aaccgctgca tcctgcaaca
       61 ttgttctatc gttccaggcc tgctcaatta tcgttttcat gccgcaaagt taatgttccc
      121 gtgggcaatt tttttatctt cgctgctgtg aataaacaat tcaaaatttt actaggtcct
      181 cttttagccg ttattgttgg gtacattttt tatgcaaccg gcaatacgcc attggtaagt
      241 aaaactgccg gtgtgcttat tttaatggca tattggtgga tgactgaagc agtaaatatt
      301 tatgtcacca gtttaatgcc tgtagtatta tttccattat tgggcattat gggtatagat
      361 accattgctc cacaatatat gaaggatgta atttttttat acatcggtgg atttatgctc
      421 acatttgcta ttgaacggtg ggatttacac aaacgttttt cattgcgcat attattaaaa
      481 ttcggttctt ctcctgaaaa attaattttt gggtttatgg ctgccacatt ttttatttcc
      541 atgtggttga ataatacgtc cactacttta atgatgttgc cggttgcatt ggccgtaatt
      601 aaacaaattc acggcaacga tatgaccaaa cgcagtggac tggcaactgc catattactc
      661 gccatttctt atgcatcaac aattggcggt acagcaacac tggtgggcac tttgccgaat
      721 atggttatga aaaaatttta cgatgaaaac ttccaggatg ccgcaccact cgattttgct
      781 aattggtttt tatttgcttt cccactgagt attgtttttc tgggatgttg ttttcttgta
      841 tttaaattta ttttacttcc aaaaagtgac catcaattac ctgataatag ttattgtaaa
      901 acggaatatc agaaattggg aaaaatgagt ttcgaagaaa aagcaattgc gattattttt
      961 tcaagcgcaa ttgttttatg gtttacgtta gaggataaag atatttgggg attacactta
     1021 acaggttgga aaacaatatt attaaatgcc ggaattattc ccgatgaaaa atttgtcaac
     1081 gaaagttttg tcgccatatt aatgggcatg agtttatttt tcttcccttc aaaaaacaaa
     1141 aaaggagaaa cattaattag ttgggaagat gtaaaacgat taccgcttgg tattttattt
     1201 ttatttggtg gcggttttgc actggctaaa ggtgttgaag tttcgggatt aagtttatgg
     1261 atagccaata attgtgcagg acttaaagta ttagatgaat gggtaatgat tttggcatta
     1321 tgtgttatca ccaccatttt ttccgaattc gccagtaata ccgcaaccgt gagtttgttt
     1381 atggctattc tgattccaat cattcctgaa ttgggcattc ctccactgtt agtcatgatg
     1441 ccggttacca ttgctgccag tttctcattt atgcttccgg ttggcactcc acccaatacc
     1501 atcgtatttg gtactgaact tattcaggta aaaacaatgc tgcgtgtagg gctgtggctc
     1561 gatttaattg gagctgttgt aattacagcc tttaccatga ctttgggaaa actgattttc
     1621 ggcatttgag gggcattatg acaaaaattt gacaatttat tttagccctt actttcttgt
     1681 tacttagatt aaatttatgt taaatttgat gtatgtaggc agggggattt ttttgagatg
     1741 gttatgatta atggatttca acctgataac ccttttacca aatgtcaatt ttattaactc
     1801 ctcaatattc aatcaatgaa aaaagtatta cttctattat ttgttgttag tcttacccaa
     1861 ttaaaagctc agttaataat taacgaggtc gcatccacta attacaatat cactcaggac
     1921 gaggaaggcg attatcccga ctggattgag ttatataatg caggtcccac tgctgttaac
     1981 ttggcgggat acgcaatttc tgatggcaaa accttggcgc cctgggtgtt taaagattcc
     2041 attattccgg caaatggacg cgttttagta tatg
//
LOCUS       DAORKW010000622         2070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3405_length_2070_cov_5.570223, whole genome shotgun sequence.
ACCESSION   DAORKW010000622 DAORKW010000000
VERSION     DAORKW010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3405_length_2070_cov_5.570223"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1663
                     /locus_tag="PLB46_16210"
     CDS             <1..1663
                     /locus_tag="PLB46_16210"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF78123.1"
                     /translation="INGDVTLTWSASAEACGGFVEYQIYASTNLAGPYILLTNIAAIG
                     TTAYTHIGADGADTTWYYYIVAVYSCPGYTMTSSDTLDNLDPIAPEIDYVTVTAGQSV
                     INWIPSVSPETNSYVIYRENGGFTPIATVYGRFTTSYTDVTGSPTTQFETYTISARDS
                     CDNIGPFNNASQHTIYLTVNQINCSDELLLNWNFYDNWTAGVFEYEIWVDRNFTGSAP
                     VDDVTNTSNSYSLTGINDGDNVCITVQAKRADGTAVAVSNEVCLLVNIVQAADYTVIR
                     NVTVNGPAQIGVEWYPDTNADLEKVSIQRSSDNIEYVNLTTYPIGLPIPVVDSYNDNS
                     IATNAISFYYRIITIDSCDNQDTSGYAKSIVLKGNDNANFTNTISWTGFEIQDGTVQE
                     YRIYRDDGAGLVLINTVGAGVQTYTDDVSMFVNVIDEFCYQIECLFRLNSPENGVDEQ
                     LLSYSNILCLEQGPRIYVPNAIVPNGVNNVFKPVLIYGTADGYNMKVINRYGEVIFET
                     DDINEGWLGTYNNKTVELGSYGYVITFTATNGQVITKKGNVTVVR"
BASE COUNT          618 a          361 c          428 g          663 t
ORIGIN      
        1 aataaacggt gatgtaaccc ttacctggag cgcatctgct gaagcttgtg gcgggttcgt
       61 ggaatatcag atttatgctt caaccaattt agccggacca tacattttat taaccaatat
      121 tgccgcaatt ggcacaaccg cttataccca tattggggct gatggggcgg atactacctg
      181 gtattactac attgtagccg tatatagctg tccgggttat acgatgacca gcagcgatac
      241 cctcgataac ctggatccta tagcgcctga aattgattat gtaacagtta ccgcaggaca
      301 atcggtaatt aactggatac catctgtttc gccggaaacc aattcgtatg tgatttacag
      361 ggaaaatggc ggatttacac ccattgccac tgtttatggt cgatttacta cttcatatac
      421 cgacgttacc ggttcaccaa ctacacaatt tgaaacgtat accatatcag ctcgagattc
      481 atgtgataat ataggaccat ttaataatgc atcgcaacac accatttatt taacggtaaa
      541 tcaaataaat tgtagtgatg aattattatt gaattggaat ttttacgata actggacagc
      601 aggtgttttt gaatatgaaa tttgggtgga cagaaatttt accggctcag ctcctgttga
      661 tgatgttacg aatacatcta attcttattc attaaccgga attaatgatg gtgataatgt
      721 ttgtattact gtgcaagcaa aacgcgccga cggaactgct gttgctgttt ctaatgaagt
      781 atgtttattg gtaaatattg tgcaggctgc tgattatact gttatcagaa atgtcaccgt
      841 aaacggtcct gcacaaattg gtgtagaatg gtatcccgat accaatgccg atttagaaaa
      901 agtatctatt cagcgcagca gcgataatat tgaatatgta aatttaacca cctaccctat
      961 tggcttgcca attcctgttg tagatagtta taacgataat tccattgcca ctaatgcaat
     1021 cagtttttat tatcggatta ttactattga tagttgtgat aatcaggata catcgggtta
     1081 tgcaaaaagt attgtgctta agggaaatga taatgccaat tttacgaata ctatttcatg
     1141 gacaggcttt gaaattcagg atggaactgt gcaagaatat agaatttacc gcgatgatgg
     1201 tgcaggactt gtattaatta atacagtcgg cgccggtgtt caaacttata ccgatgatgt
     1261 ttctatgttt gtaaatgtta tcgatgaatt ctgttatcaa attgagtgtt tattcagatt
     1321 aaattctccc gaaaacggtg ttgatgaaca attactttct tattcgaata tcctctgttt
     1381 agaacaaggt ccaagaattt atgtaccaaa tgctattgtt cctaatggtg taaataatgt
     1441 tttcaagccg gtattaattt atggcaccgc cgatggttat aatatgaaag ttataaatcg
     1501 ttatggtgaa gtaattttcg aaactgatga cataaatgaa ggctggttag gcacctacaa
     1561 taacaaaaca gttgaattag gttcgtatgg ttatgtaatc acatttactg ctacaaatgg
     1621 acaagtgatt acgaaaaaag ggaatgttac ggtggtgagg taatgtgctg atgtgttaat
     1681 gtgctaatta atgtaccaat gtgtgaatgt accgatgtac caattaatgt atccgctgag
     1741 gcggatgata atgtgctaat gcgaagcatc cgccgaggcg gaaacagccg ggttatgaat
     1801 tgaaatttaa ttcgaagtcc ctgtagtagt caacccacgt gttgaccttt ttaatcatta
     1861 attcaaacag ccgtggttca caaagggcgc caaaaattgc gcaaaaagcg caaaggatta
     1921 tattaattca ttttatttag tgccttttgt gtcggttcat tttgtgttcc ggtgttccgg
     1981 aattgcgggc taaaaaacca tccctttcat atggaatata taccatttca cgctgtgaac
     2041 tttccgggct gttttctttg ggtttcctct
//
LOCUS       DAORKW010000623         2070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3406_length_2070_cov_5.265012, whole genome shotgun sequence.
ACCESSION   DAORKW010000623 DAORKW010000000
VERSION     DAORKW010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3406_length_2070_cov_5.265012"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="PLB46_16215"
     CDS             <1..1304
                     /locus_tag="PLB46_16215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF78124.1"
                     /translation="NVCLTVENIAGVDSECKDVIIANFLAPEVNFASTGDPDVMFTDL
                     TLNDPDSWLWSFGDGYSSTEQNPVHTYSENGIYSVCLWASNDYGSNSGCKMIEINGLM
                     ELPESFFSFDGEPEVVFADLSSGAPAEWFWDFGDGTFSTEQNPSHLFVENGTFEVCLT
                     ASNIMGEHTYCEVVIIDSYMAPISAFSYTGDPNVNFTDLSSNMPNSWLWDFGDGSFST
                     LQNPIHTFITNGLFTVSLTSANGGGVSTNCEDVAIIGCLPAPVCAFNYTIIDNIIYFD
                     DLSDYSPESWQWYFGDGSSSELESPAHIYVEPGTYEVCLTVQNQSGEAVCCQTINTVD
                     VSVPLQQSNYLEIFPNPSPDIVRMKIPQNEITGNAALTVIAMDGRLVYPKYEQMNANE
                     IMISFQDLPNGVYQINYMVINGVYTAIVVINGVYTAIVVIN"
     gene            1863..>2070
                     /locus_tag="PLB46_16220"
     CDS             1863..>2070
                     /locus_tag="PLB46_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78125.1"
                     /translation="MKQFFGPLLCTLVAVSGFAQPASYFTSDLEGWTALCECANDPNI
                     SWGSIAGAPGGAAKGTDDANGTWYF"
BASE COUNT          606 a          344 c          404 g          716 t
ORIGIN      
        1 ataatgtgtg tttaacagtt gaaaatattg ccggtgttga ttccgaatgt aaggatgtta
       61 ttatcgcaaa ttttttagca ccggaggtaa attttgcttc aaccggggac cccgatgtaa
      121 tgttcactga tcttacatta aacgacccgg atagttggtt atggagtttc ggcgatggat
      181 attcatctac cgaacaaaac cctgtacata cttatagtga aaatggaatt tactctgttt
      241 gtttatgggc aagtaacgat tatggatcca actccggttg taaaatgatt gaaataaatg
      301 gactcatgga attgccggaa tcatttttct catttgacgg tgaacctgaa gttgtttttg
      361 cagacttaag cagtggtgct cctgctgagt ggttttggga ttttggcgat ggcacatttt
      421 caactgaaca aaacccttca cacttgtttg tcgaaaatgg aacgtttgaa gtttgtttaa
      481 ctgcatccaa tattatgggt gaacatacct attgtgaggt agtgataatt gatagttata
      541 tggccccgat ttcagcattt agttataccg gtgaccctaa tgtaaatttt acagacctat
      601 catcaaatat gcctaactca tggttgtggg attttggtga tggtagtttt agcacattac
      661 aaaatcctat acatactttc ataactaatg gattgtttac tgttagttta acctcagcaa
      721 atgggggtgg ggtgagtacc aattgtgaag atgttgctat aattggttgc ttgccagctc
      781 ccgtttgcgc ttttaattac actattattg ataatatcat ctacttcgat gatttgtctg
      841 attacagtcc tgaaagttgg cagtggtatt ttggcgatgg aagttcgtct gagctagaat
      901 cacctgcgca tatttatgtt gaacccggca cttatgaggt ttgcttaaca gttcaaaatc
      961 aaagtggtga agcggtttgc tgccaaacta ttaatacagt tgatgttagt gtcccattac
     1021 agcaatcaaa ctatttagaa atatttccaa atccttctcc tgatattgta cgtatgaaaa
     1081 ttcctcaaaa tgaaattacc ggcaatgcgg ctttgaccgt gatagctatg gacgggagat
     1141 tagtttaccc taaatacgaa caaatgaatg caaatgaaat aatgatttcg tttcaagatt
     1201 taccaaatgg tgtatatcaa atcaattata tggtaattaa tggggtgtat acagcaattg
     1261 tggtaattaa tggggtgtat acagcaattg tggtaattaa ttaaatgcaa taattgtttt
     1321 accgcaatca aaatacatta actatttaat gcagcatagt tattgtcctc tcaattattt
     1381 tacagcttta ttcatcttct atttactaaa gccattttgg gttagttaac ttatgttgta
     1441 caatgtatta gtcatgttac caggttatga aattacaatt tggaaccttt taatggcatt
     1501 gactggtcat ttttaacaaa aaaaatcgta tattaaagta tacaccaaag taagaactgc
     1561 taactttgga gcgaaaatag cctatcacaa aaaattgata tttgttaatc tttttttaac
     1621 atgaatttac gctatttaca ggtacttgaa gtacttagcc tttgttgttg cttttttttt
     1681 aacaattagc ctaaattcct atattttttg cataatatgc gggtttgtaa ctgtttcacg
     1741 attcacggaa aaccgttaat ttgttatcat ttgagaattt tttttcaact gtactttgat
     1801 tttcaacatg ggaatactta tttttgacag ctgattttac caaagtcaca aacacacaca
     1861 ttatgaaaca attttttgga cccctgttat gcacactggt agcggtatct ggctttgcac
     1921 aacctgcgtc gtatttcaca agcgaccttg aaggttggac tgctttatgc gaatgcgcaa
     1981 atgaccctaa tattagttgg ggttctatag cgggcgcacc cggcggtgcc gccaaaggca
     2041 ctgatgatgc caacggaact tggtatttca
//
LOCUS       DAORKW010000624         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3419_length_2066_cov_2.700646, whole genome shotgun sequence.
ACCESSION   DAORKW010000624 DAORKW010000000
VERSION     DAORKW010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3419_length_2066_cov_2.700646"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PLB46_16225"
     CDS             complement(<1..232)
                     /locus_tag="PLB46_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRF78126.1"
                     /translation="MQQQYIAKTLQGLENVLAKELETLGAKDVEVLKRAVSFSGDKAM
                     LYAANIYLRTAIKILQPLTTFRADDVQELYDQI"
     gene            complement(315..851)
                     /locus_tag="PLB46_16230"
     CDS             complement(315..851)
                     /locus_tag="PLB46_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78127.1"
                     /translation="MKKLYNLTLLIAFVTLFSVTGNSQVVMKDYLVANHQGVVEKSLN
                     NNGSGLFYKFEYVSTEGARINYKLHLYKDKGMSTPWMSFDVLMRNLNWTYYVDITMSK
                     DGADKVAAMIFKKDLRWSRVKYSPHVGCLRVDPPVYERYTIGAETESAEVLFAGLLNN
                     FIAQLDKNVDFNCYAAAK"
     gene            complement(872..>2066)
                     /locus_tag="PLB46_16235"
     CDS             complement(872..>2066)
                     /locus_tag="PLB46_16235"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HRF78128.1"
                     /translation="SPPDAARMYAYIAVGMYEAQIAGSPDYLTLEGQLADLKGLPRPE
                     KGQVYDWQTCVTETMYLVQDGMLARYFPAGVSTLNKLYDKQIEERKAAGVTDEVLERS
                     KAYGEELADVILEWSEADKYVETRYMQYKSPTREGHPNYWEPTDFNQTPLEPFWGTHR
                     TFFVKDGTQCDIDKAPAYGGKGDTTGELYKQAKKVWTVDKTLTEEQRTIAQYWADDPS
                     ETSTPPGHWMEILGNFVKSENMKLDRACELYALVTTSMNDACITVWHTKYRVNLVRPK
                     TVIQETLDSKWEPYVETPPFAGYTSGHSGFSGAAATILTSRIGDNLAFIDSSHVDIGL
                     LPRNFKSFNDAAQEAAYSRMYGGIHFDCDIDDGLEQGKCVGNYYLENIKTKAAHTEKK
                     GTEAK"
BASE COUNT          606 a          434 c          360 g          666 t
ORIGIN      
        1 gtatttggtc atacaattcc tgcacatcat ctgctctaaa agtagtgagt ggttgtaaaa
       61 ttttaatagc tgttcgcaaa tagatattgg ctgcatacaa catcgcttta tcgccggaaa
      121 acgacactgc tcttttcagc acttctacgt ccttagctcc aagtgtttca agttctttgg
      181 ccagcacatt ttccaaacct tgcagtgttt ttgcgatata ttgttgttgc ataaagtaat
      241 aatctgatac aaagtaaaat aaaagaggcg ttgtttttat tacaacgcct cttttaatat
      301 gctaaaggtg gaaattattt cgccgcagcg taacaattaa aatccacgtt tttgtctaat
      361 tgagcaatga agttgtttaa caaaccggca aataatactt cagccgattc agtttctgca
      421 ccaatagtat aacgttcgta taccggtggg tcaactctca aacaaccaac gtgtggagaa
      481 tatttaacac ggctccaacg taagtctttt ttgaaaatca tcgcagcaac tttatctgca
      541 ccgtctttac tcatggtaat atccacgtag taagtccagt taagattgcg cattaaaaca
      601 tcaaaactca tccaaggtgt agacattcct ttgtctttgt ataaatgtag tttataattg
      661 atacgggcac cttcagtgct tacatattca aatttataga ataaaccact tccattgttg
      721 tttaatgatt tctcaacaac accctgatga ttggctacca aatagtcttt cataactacc
      781 tggctattac ctgtaacgct aaataaggtt acaaaggcaa ttaataatgt aaggttatat
      841 aattttttca tagttgctat tcgcttttag gttatttagc ttctgtacct tttttctcag
      901 tatgagcagc ttttgttttg atattttcaa gataataatt acccacacat ttaccttgtt
      961 ctaaaccatc atcgatatcg caatcgaagt ggataccacc atacatacgg ctgtaagcag
     1021 cttcttgtgc cgcatcgtta aatgatttga agtttcttgg caataaacca atatcaacgt
     1081 gtgaactatc aataaaagct aaattgtcac caatacgtga agttaaaatg gttgcagcag
     1141 caccactaaa tcctgagtga cctgaagtat aacctgcaaa cggaggtgtt tcaacatacg
     1201 gttcccattt tgaatctaat gtttcctgaa taacagtttt aggacgaaca agattaacac
     1261 ggtatttagt atgccaaaca gtgatacatg catcattcat tgaagttgta accaatgcat
     1321 acaattcgca agcacggtcc aatttcatgt tttcagactt aacgaaatta cccaaaattt
     1381 ccatccagtg gcccggtgga gttgaagttt cacttggatc atcagcccag tattgagcaa
     1441 tagttctttg ttcttcagtt aaagttttat ctacagtcca aactttttta gcttgtttgt
     1501 ataattcgcc tgtagtatca ccttttccac cataagccgg agctttatca atatcacact
     1561 gagtaccatc ttttacgaag aaagtacggt gagttcccca gaaaggctca agtggggttt
     1621 gattgaaatc tgttggttcc caatagttag ggtgaccttc acgagttggt gacttatact
     1681 gcatgtaacg tgtttccaca tatttgtcag cttcactcca ctcaagaatc acatcagcta
     1741 attcttcgcc ataagccttt gagcgttcta acacttcgtc agttacgccg gcagctttgc
     1801 gttcttcaat ttgtttatcg tataatttgt ttaacgtact aaccccagca gggaaataac
     1861 gcgctaacat accgtcctga actaaataca tggtttcagt aacgcatgtt tgccagtcat
     1921 aaacctgccc cttttccggg cgaggaagac cttttaaatc agccaattgg ccttcaagtg
     1981 ttaaataatc agggcttcct gctatttgtg cctcatacat accaacggca atataggcat
     2041 acatgcgcgc tgcatcaggc ggtgat
//
LOCUS       DAORKW010000625         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3424_length_2063_cov_3.644422, whole genome shotgun sequence.
ACCESSION   DAORKW010000625 DAORKW010000000
VERSION     DAORKW010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3424_length_2063_cov_3.644422"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLB46_16240"
     CDS             complement(<1..762)
                     /locus_tag="PLB46_16240"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin"
                     /protein_id="HRF78129.1"
                     /translation="MYSKDKWIVLLLPVLYITAGCSNHPKNAVESPATQLADLYDTLS
                     TPQPGEWLYEQQEFGQSLEEFRDETPIDSNNADATFYILPIDIIHEEEFSLIADVATY
                     LTTIFGYEVTVMPSMTAQLFPPHYFRDEQLNSQLLLDEIIRPLLEQDALGLMGITQRD
                     IYPGDGWNFVFGQANTKEKIGITSFARYGDYDTDSARQLVLNRLIKTTTHEFLHMLGL
                     QHCIQFACVLNGSNSLEESDKKKSIICPECLAKLEI"
     gene            complement(746..>2063)
                     /locus_tag="PLB46_16245"
     CDS             complement(746..>2063)
                     /locus_tag="PLB46_16245"
                     /inference="COORDINATES: protein motif:HMM:NF020678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HRF78130.1"
                     /translation="NAKNSRVKIRATGHGFGGTANCSEFCARDHYFKVNGSTVWSKEV
                     WRDNCDVNPVYPQGGTWVYDRANWCPGAEVWTYDYELTPEVTPGNTYTFDYSAEDYTW
                     NNVGSVPTYVTSVQLVTYGDPNFNLDARLDAIIAPSDDDMQQRKNPICNNPIVRITNT
                     GATPLTKLDITFGIKDYDQDTYTWTGNLAFMESEEVTLPNFGWNGSGTEFIATISSPN
                     GGTDEYVYNNSITTPLAIPPTYPSEFIVETRTNTASHENDLFVYDENGNTILERTSFS
                     DNTVYKDTLILEDGCYELYLWDYGEDGLSWWANSDGSGYFRTKEIDGTFIKSFEADFG
                     GLIYMQFIVGNYTPVETFVKPADEVNLYPNPAQNEIFIDLDFAQLSDADITVADITGK
                     TIHTQSVQRVQDETVHFGMENQAAGVYVVTIKMSDKIITKQFVLER"
BASE COUNT          628 a          426 c          353 g          656 t
ORIGIN      
        1 gatttcgagt tttgccaaac attccgggca aatgattgat ttttttttat cggactcctc
       61 caatgaatta ctgccattta aaacacaagc aaactgaata caatgttgta gtcccaacat
      121 gtggagaaat tcatgtgtag tggtttttat cagtctattc aatactaatt gacgagcact
      181 atctgtatca taatcaccat atcgtgcaaa ggaagtaata ccaatttttt ctttggtatt
      241 ggcctgacca aatacaaaat tccaaccatc gccgggataa atatcgcgtt gggtgatacc
      301 cattaatccc aaagcgtcct gctccaataa gggacgaata atttcatcta acaacaactg
      361 actattgagt tgttcatcac gaaaataatg gggtgggaat aattgcgcag tcatagaagg
      421 catcaccgtt acctcataac caaaaatggt tgttaaatac gtcgccacat cggcaatcag
      481 cgaaaattct tcctcatgaa taatatcgat gggcaaaata taaaaggtag catcagcatt
      541 attgctatca attggtgttt catcgcgaaa ttcttcgagg gattgaccaa actcctgttg
      601 ctcatataac cactctccgg gttgaggtgt ggaaagggta tcatataaat cggccaactg
      661 agttgccggc gattcaacag cattttttgg atgattactg catcccgctg tgatatacaa
      721 aacgggaagc agtaatacta tccatttatc tttcgagtac aaattgtttg gtgattattt
      781 tatccgacat ttttatcgtt accacatata cacctgctgc ctgattttcc attccgaaat
      841 gtacggtttc atcttgtaca cgttgtacac tttgagtatg aattgttttt ccggtaatat
      901 ctgcaacagt gatatccgca tcgcttaatt gtgcaaaatc caaatcaata aatatttcat
      961 tttgagcagg gttaggatat aaatttactt catcggcagg tttaacaaaa gtttcaaccg
     1021 gagtataatt acccacaata aattgcatgt aaatcaaccc accaaaatcg gcttcaaatg
     1081 atttaataaa tgtgccgtca atttcttttg ttctgaaata tcccgaacca tcactatttg
     1141 cccaccagct taatccatct tcaccgtaat cccataaata caattcgtaa caaccatctt
     1201 caagaattaa tgtgtcttta taaacagtat tatcagaaaa agaagttctt tctaaaatag
     1261 tattcccatt ttcatcatac acaaataaat cgttttcgtg tgatgctgta ttcgttcttg
     1321 tttctacaat aaattccgaa ggataggtcg gtggtatagc caatggtgta gtgatagaat
     1381 tgttatatac atattcatct gttcccccat tcgggctgct tatggttgca ataaattcag
     1441 ttcctgaacc attccatccg aaattaggta aggtaacttc ttcactttcc ataaaagcta
     1501 aattaccggt ccaggtataa gtatcctggt cgtaatcttt tattccaaat gtaatatcta
     1561 atttagttaa aggtgtagct cctgtattgg taatgcgcac tatcggatta ttacaaattg
     1621 gatttttgcg ttgttgcata tcatcatcgc ttggtgcaat aatggcatct aatctggcat
     1681 ctaaattaaa attcggatca ccgtatgtaa ctaattgtac cgaagtaaca taagtaggca
     1741 cactaccaac attattccat gtataatctt ctgctgaata atcaaatgta taggtatttc
     1801 ccggagtaac ttcaggtgtt aattcataat cgtacgtcca tacttctgca cccggacacc
     1861 agtttgcccg gtcgtacacc caagttccac cttgcggata aacagggtta acatcgcagt
     1921 tatctctcca aacttcttta ctccaaacag tgctgccatt tactttaaaa taatggtcgc
     1981 gcgcacaaaa ttcagagcag ttagcagtgc ccccaaaacc gtgaccggta gcgcgaattt
     2041 ttacacgtga attttttgca ttg
//
LOCUS       DAORKW010000626         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3438_length_2056_cov_4.320340, whole genome shotgun sequence.
ACCESSION   DAORKW010000626 DAORKW010000000
VERSION     DAORKW010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3438_length_2056_cov_4.320340"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="PLB46_16250"
     CDS             complement(<1..1770)
                     /locus_tag="PLB46_16250"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRF78131.1"
                     /translation="MAFDIEMIKQVYANYPSKIAAARAILNRPLTLTEKILYAHLSEG
                     LATKNYVRGKDYVDFGPDRVAMQDATAQMALLQFMSAGKKKVAVPSTVHCDHLIQAKV
                     GAQQDLAAALESSKEVFDFLASVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMVAIGVGGADAVDVMAGFPWELKMPKLIGVKLTGKMSGWTSAKDVILK
                     VAGILTVKGGTGAIVEYFGEGAESISCTGKGTICNMGAEIGATTSVFGYDDSMRRYLN
                     ATDRSDIVALADTVAAHLNGDAEVYANPSAYFDQVIEINLSELEPHVNGPFTPDLAWP
                     ISKLKDAVIANNWPAELEVGLIGSCTNSSYEDLDRAASLAKQAVAKKLKVKSEFTITP
                     GSEQVRYTVERDGILADFEKIGGVVLANACGPCIGQWARHSNDPERKNTIITSFNRNF
                     AKRNDGNPNTHAFVASPEIVTALAIAGSITFNPLTDTLINEDGVAVMLDEPKGLELPT
                     KGFAVEDAGYIEPAVDGSAVNVIVAADSKRLQLLAPFKAWEGTDLKGLKLLIKAKGKC
                     TTDHISMAGPWLKFRGHLDNISNN"
BASE COUNT          615 a          462 c          349 g          630 t
ORIGIN      
        1 attattagaa atgttatcta agtgtccgcg gaattttaac catggacccg ccattgaaat
       61 atggtcggta gtacattttc cttttgcttt gattaataat tttaacccct ttaaatcggt
      121 accttcccat gctttaaatg gcgctaataa ttgcaaacgt tttgaatccg cagcaacaat
      181 tacattaact gcactgccat caacagccgg ttcaatataa cctgcatctt caacagcaaa
      241 acctttggtt ggtaattcta aaccttttgg ttcgtctaac atcaccgcaa caccatcttc
      301 attaattaat gtatcggtaa gcggattaaa tgtaatagaa cctgcaatgg ctaaggcagt
      361 tacaatttcc ggtgacgcaa caaatgcatg cgtattcgga ttaccatcgt tgcgttttgc
      421 aaagtttctg ttgaaagaag taataatggt attttttctt tccgggtcgt tgctgtgacg
      481 tgcccactga ccaatacaag gtccgcaagc gttggctaac acaacaccac caattttttc
      541 aaaatcggct aaaataccat cgcgttcaac tgtatatctc acttgttcgc tgccaggggt
      601 gatggtaaat tctgatttaa cttttaattt ttttgctact gcttgttttg ccaatgaagc
      661 agcacggtct aaatcttcat aactggaatt ggtgcaagaa ccaatcaaac ctacttctaa
      721 ttctgcaggc cagttattcg caataaccgc atcttttaat tttgaaatcg gccaagctaa
      781 atccggtgta aaaggaccat ttacatgtgg ttctaattca ctcaaattaa tttcaataac
      841 ctgatcaaaa taagcagatg gattagcata tacttctgca tcaccattta aatgtgcagc
      901 aacagtatct gctaaagcaa caatatcaga acgatcggtt gcatttaaat atcggcgcat
      961 gctatcatcg taaccaaata cagatgttgt tgcaccaatt tcagcaccca tattacaaat
     1021 tgtgccttta cctgtgcaag aaatactttc tgcaccttca ccaaaatatt ctacaattgc
     1081 accggttcca cctttaacgg tcaaaattcc ggcaactttt aaaatcacat ctttagcact
     1141 ggtccagcca ctcattttac cggtgagttt cacaccaatt aatttcggca ttttaagttc
     1201 ccaagggaaa cctgccatca catccacagc atcggcacca ccaacaccaa tcgctaccat
     1261 acctaaacca ccggcattaa cagtatgact gtcggttcca atcatcatac cacccggaaa
     1321 tgcataattt tcaagcacta cctggtgaat aatacctgca cccggtttcc aaaaaccaat
     1381 accatatttg tttgaaacgg aagccaaaaa gtcgaatact tctttgctgc tttccagtgc
     1441 agctgctaaa tcttgttgtg caccaacttt tgcctgaata aggtggtcgc aatgtacggt
     1501 tgaaggaacc gctacttttt tcttacctgc gctcataaac tgcaacaacg ccatttgagc
     1561 agtagcatcc tgcatggcaa cacggtccgg accaaaatcc acataatcct tgccgcgcac
     1621 atagtttttc gtcgccaaac cctcgctcag gtgagcatac aatatctttt cagtgagggt
     1681 taaaggacgg ttaagaatag ctcttgctgc agcaattttt gaaggataat tggcataaac
     1741 ctgtttaatc atttcaatat cgaaagccat attggtaagg tgttttttga attcgtctgc
     1801 aaaggtaaca aacagaggtc attattagtt tattaaacga ataaaaatta ttttttagac
     1861 ttgtctaaaa atattaaaac gatatgtttt ataaattttg tgaaaagaag taattttggt
     1921 aaaactccat tatgaaaatc acttcacttg ctgcctattc cgctgcgtcg ggattgttgc
     1981 tcggtctcac ttctgaggct tctgcccaaa tgacatatta taacattgac cctgatgcta
     2041 cagtttttgg tgatat
//
LOCUS       DAORKW010000627         2055 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3442_length_2055_cov_2.921000, whole genome shotgun sequence.
ACCESSION   DAORKW010000627 DAORKW010000000
VERSION     DAORKW010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3442_length_2055_cov_2.921000"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLB46_16255"
     CDS             complement(<1..307)
                     /locus_tag="PLB46_16255"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRF78132.1"
                     /translation="MKLLKKFMPVILLVASSHGVFGQLSPWWAQTDSIDSAEVKREVI
                     ADSIINFAKTFMGTPYVWGGNSPDSGFDCSGFVCYVYKHFNIALPRTSGQQFDAGTPI
                     "
     gene            complement(311..775)
                     /locus_tag="PLB46_16260"
     CDS             complement(311..775)
                     /locus_tag="PLB46_16260"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HRF78133.1"
                     /translation="MSKKTKNIDTTNIRALTPFLPDNLFVAKTPDRGRAVFTSKLIPA
                     GTVIEIAPMIVFNEKDRLLLNDTFLYEYYFEWGKSGRKGALALGFGSLYNHSYAPNAR
                     YLPDFDLNILEFVAVRDIQPGEEICVNYNLDPDDQTPVWWERDKIKKKKQHP"
     gene            complement(784..1038)
                     /locus_tag="PLB46_16265"
     CDS             complement(784..1038)
                     /locus_tag="PLB46_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78134.1"
                     /translation="MSKRSAKKRIVNGIASAIDQLILTTGNDERQFDEKTEQLFKLYD
                     EAMTKINATAKLKDRSAVKQHYKSLYADLQAGINAVTGKK"
     gene            1099..1410
                     /locus_tag="PLB46_16270"
     CDS             1099..1410
                     /locus_tag="PLB46_16270"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRF78135.1"
                     /translation="MFDLKKAMEMKKKMDEVQARLENISVDGESGDGKYKVQVTVTAT
                     KKVKSILISDELMQNGDKEHLQDLLLIAIERAMDKAQNVAEAEARSTAMGGGLSGLFG
                     M"
     gene            complement(1419..>2055)
                     /locus_tag="PLB46_16275"
     CDS             complement(1419..>2055)
                     /locus_tag="PLB46_16275"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRF78136.1"
                     /translation="FCWMYMNGSPSSPPTKLPVAIIDLFAAHQLKEGVLTALIKKLKT
                     GRGSKVSVSLFDVAVSSLANQAANYLNTGVFPERIGSLHPNIAPYGEIITFKDGEQVV
                     LAIGSDRQFRALCKIAGCTDLCGVDKYATNADRVINRNTLHGLLQSKMQKMTSEEFIY
                     ACRQDNIPVGIIRNMEQLFELESAQRLILEDENGQRVKSVVFKIEEKQLVS"
BASE COUNT          597 a          406 c          424 g          628 t
ORIGIN      
        1 gaattggtgt tccggcatca aattgctgtc ctgaggtgcg aggaagtgct atattaaaat
       61 gcttgtatac ataacaaaca aaaccggagc agtcgaaacc ggaatcaggt gaatttccgc
      121 cccaaacata gggtgtcccc ataaaagttt tagcaaaatt gatgatgctg tcggcaatta
      181 cttcgcgctt aacctcagct gaatcgatgc tgtccgtttg tgcccaccag ggagaaagct
      241 gcccaaaaac accatgcgag ctggcaacta gaagtatgac cggcatgaat tttttgagca
      301 gcttcataat ttaagggtgt tgctttttct ttttaatctt gtcgcgttcc caccaaaccg
      361 gcgtttgatc atccgggtcg agattgtagt taacacaaat ttcttcgccc ggctgaatat
      421 cacgaacggc aacaaattca agaatattga ggtcgaaatc aggcaaatag cgtgcatttg
      481 gtgcatagct atggttataa agtgagccga atccgagcgc caaagcacct tttcgtccac
      541 ttttacccca ttcgaagtag tattcatata aaaatgtgtc attgagtagc agtctgtctt
      601 tttcattaaa aacaatcatg ggtgctattt caataactgt gcccgccgga attaacttgc
      661 tggtaaacac tgctcttccc ctatccggtg tttttgcgac aaacagatta tcaggtaaaa
      721 aaggtgttaa tgctctgatg ttagtggtat ctatgttttt cgttttttta gacatcagat
      781 ttattatttt ttgcccgtaa cggcgttaat accagcctgt aaatcggcgt aaagcgactt
      841 atagtgttgt tttacagcag aacggtcctt caattttgcc gttgcattga ttttggtcat
      901 agcctcatca tacaacttaa ataattgttc tgttttctca tcgaactgac gttcatcgtt
      961 tccggtggtc aggattaatt ggtcgatagc ggaagcaatt ccgttaacga ttcgtttttt
     1021 tgcactgcgc ttgctcattg ataaaatttg cggcaaaatt acgcatatat ctatctttgc
     1081 acaaaaaata cccaaactat gtttgatttg aaaaaagcga tggagatgaa aaagaagatg
     1141 gatgaggttc aggctcgtct cgaaaacatc tcagttgacg gtgaatccgg agacggcaag
     1201 tataaggttc aggtaactgt tacggcaaca aaaaaagtga aatcgatact gataagtgat
     1261 gagttaatgc agaatggtga taaggaacat ttacaggatt tgttattaat tgccattgaa
     1321 cgtgccatgg ataaagcaca aaatgtagca gaagcagaag ctcgttctac agcaatgggt
     1381 ggaggcttaa gcggtttatt tggcatgtga agatgcggct agcttaccag ttgtttttct
     1441 tcaattttaa agacaacact tttcacacgc tggccatttt catcttctaa tatgaggcgt
     1501 tgagcgcttt ctaattcaaa cagctgctcc atgtttcgaa ttatgccaac aggaatatta
     1561 tcttgcctac aggcataaat aaattcttca ctggtcattt tttgcatttt actttgcaat
     1621 aagccgtgta gggtatttct gtttattact ctgtcggcat tggttgcata tttatctaca
     1681 ccacataaat cggtgcaccc ggcaattttg cacaatgccc taaattgcct gtcggagcca
     1741 attgccagca ctacctgttc gccatcttta aatgtaatta tttctccata aggagcaata
     1801 ttgggatgca gtgaacctat acgttcagga aaaactccgg tatttaaata attagccgcc
     1861 tgattagcta aggaagaaac tgcaacatca aataaactca cgctcacctt ggagcctctg
     1921 ccggttttta attttttaat taatgcggta agcacacctt cttttagttg atgagcagca
     1981 aataaatcta taatagcaac gggcaatttt gttggaggag atgaaggtga accattcata
     2041 tacatccaac aaaat
//
LOCUS       DAORKW010000628         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3450_length_2050_cov_3.482206, whole genome shotgun sequence.
ACCESSION   DAORKW010000628 DAORKW010000000
VERSION     DAORKW010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3450_length_2050_cov_3.482206"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1708
                     /locus_tag="PLB46_16280"
     CDS             107..1708
                     /locus_tag="PLB46_16280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HRF78137.1"
                     /translation="MKNENIIVELSGASSATRGKTVKSLEKTGLPLTPIFEGKKLRGS
                     EPEQAKWNVYTTKENQNNWDAAHEVVSKAGKDVITYAEPDKLYINPFDVTKKRAGSKG
                     KHHGDYLSEWAQPKSNEFAWYLDKSNLKKIRHDDPGKTGTKIRIAHFDTGYDGDNHES
                     KPLYLKPELGYNFIEGGLPTDKNNTSIKNQPGHGTATMAILAGNKVDLSPDWNDYMGG
                     APFAEVIPFRISDSVILLKAVEFAQALEMAMQLKCDVVTMSMGGLASKLWAEKVNQAY
                     DAGMVVVSAAGNNFGGGLPTHHVIYPSRFNRVITACGVTYNNTPYYNKKYGHKVMMGN
                     WGPDSVMHTAVAAYTPNVPWATMKVRGDGFGKGGGGTSSATPQVAATAALFLEKFNNY
                     KFAKPWMRAEAARMAILESAKFVEANKKYFGCGILDAEAALNYGVAKTLSKADSDDVR
                     LPFIQVMLKRRGPGLNDRNEMFETEILHLIELDANINMWAVKKNAIDKMKYDVLSKKE
                     MTELRDLILASKLASDAIKSRFKSL"
BASE COUNT          738 a          306 c          412 g          594 t
ORIGIN      
        1 agaagcacct gtgacacaaa aagataaaat agttgtgttg gaaactgtac atcaaaaact
       61 ttcaggcatt tttgttgaag attaaatcac cttaaaaaaa ataaatatga aaaacgaaaa
      121 catcatagtt gaattgagtg gtgcttcaag tgccacgaga ggtaaaacag ttaaatcgtt
      181 ggaaaaaact ggtttgccgt taacacccat ttttgaagga aaaaaactgc gtgggagtga
      241 acctgaacaa gcaaaatgga atgtgtatac aaccaaagag aatcagaata attgggatgc
      301 agcacacgaa gtggtatcaa aggcagggaa agatgtaatt acgtatgccg aacctgacaa
      361 gttgtatatc aatccttttg atgtaactaa aaaacgagcc ggcagcaaag gaaaacatca
      421 tggtgattat ttatcggaat gggcacaacc aaaatcaaac gaatttgcct ggtatttaga
      481 taaatcaaat ttaaaaaaga tcagacatga tgatcctgga aaaaccggca ctaaaataag
      541 aatcgcccat tttgataccg gatatgacgg agataatcat gaatcaaaac ctttatacct
      601 taaaccagaa ttagggtata attttattga gggaggattg cctacagata aaaataatac
      661 cagtataaaa aatcaaccgg gacatggaac ggcaacgatg gcaattttag caggtaataa
      721 agttgattta agtcccgatt ggaatgatta tatgggggga gcaccatttg cagaagtaat
      781 cccattccga atttccgatt cagttatatt attgaaagca gtagaatttg ctcaagcact
      841 tgaaatggcc atgcaattaa aatgtgatgt agtaactatg tcaatgggcg gacttgcgtc
      901 taaactatgg gcagaaaaag ttaatcaggc ttatgatgca ggtatggttg tagtgagtgc
      961 tgccggaaat aatttcggtg gtggtttgcc aacgcatcat gtaatttatc cttcgcgatt
     1021 taacagggta attacagcat gtggcgttac gtataataat acaccttatt acaataaaaa
     1081 atacggacat aaagtaatga tgggtaattg ggggccagat tcggtaatgc atactgccgt
     1141 tgctgcatac acaccaaatg taccgtgggc aactatgaaa gttagaggtg atggctttgg
     1201 taaaggggga ggaggtactt cttcagcaac accacaagta gctgcaaccg ctgcgttgtt
     1261 tttggaaaaa tttaacaatt acaaatttgc taaaccgtgg atgcgggctg aagcagcaag
     1321 aatggctata ttagaatccg caaaatttgt cgaagctaat aaaaaatatt ttggttgtgg
     1381 tattttggat gccgaagcag ctttgaatta tggtgtggca aaaactctat ccaaagcaga
     1441 ttctgatgat gtgcgattgc cttttattca ggtaatgtta aaacgcagag gtcctggctt
     1501 aaacgaccgt aacgaaatgt ttgaaacaga aattctacat cttattgaat tggatgccaa
     1561 tataaatatg tgggcagtaa aaaagaatgc tatcgataaa atgaagtatg atgtattatc
     1621 caaaaaagaa atgaccgaat tgcgtgattt aattcttgca tcaaaattag catccgatgc
     1681 tattaaatct cgattcaaaa gtttataaaa acaggcttaa aaatggattg aaattgaagt
     1741 aaataattca attgcaatcc acctgaaatt ttgacagatt ctctaaaacc ttccaggttt
     1801 aaacctggaa ggtctaaatc tggaaggttg tattattttt atttgtgttg caagcattta
     1861 attttaacac ccaactaagt ttaactcaaa ttgtaataaa atagaatcat tgattttaat
     1921 attttttact aactgcaatt ctggcagtgt cattttttag ccaatgcttt ttctaaatat
     1981 tcttttaatt aatttaatag taattattta attaaatgta attgcatatt gtaaatatta
     2041 caataagtat
//
LOCUS       DAORKW010000629         2048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3456_length_2048_cov_4.235825, whole genome shotgun sequence.
ACCESSION   DAORKW010000629 DAORKW010000000
VERSION     DAORKW010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3456_length_2048_cov_4.235825"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PLB46_16285"
     CDS             complement(<1..1019)
                     /locus_tag="PLB46_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78138.1"
                     /translation="MQKTFTLLFLFVSLLSYQSFSQVCSTCSIDYAWISPGVYPDTLP
                     PATAGEFYESDITFVMQADTTVDVVGTLEFLNYHILEPVGMPYGMNVSTNLGDLPVDY
                     DPDVSLYGCARVCGTPLVPGWYEVTVPLIATLEYPGGDQAAEYSIFLEVLPAVPEDGG
                     IIPSLTYGCDPINVDFETSIHSDGVDGFTYSWDFGNGVTSSSEFPPTQTYTAVGGVPT
                     DYVVSHTITIDTFGYQLEYATIVSSGCDDCTIFGCTGLIEAEKPDLYLIITALGVNTE
                     ADAFVDTSPPVTISLGVALDPGATYTMQMKDDDGGLAGGDDNCGSFTFGGDDVGISTL
                     TAGSHT"
     gene            1432..>2048
                     /locus_tag="PLB46_16290"
     CDS             1432..>2048
                     /locus_tag="PLB46_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF78139.1"
                     /translation="MERKNFLRSLGLLAAGTMVPLKSKSVVTINEPLPTNQRLMRIAH
                     LTDAHIADNKNARNGFIKCLHAIQQMEDTPDYIINGGDAIEDALYNTKSNVNKQWDLW
                     HSILKNECSIKVNNTIGNHDIWGLYTEKKDFLYGKKFAQEKLQMEHTYYSVDQCGWHI
                     IFLDSTQKKSNGMWYTAKLGEEQIDWLSRDLATTPANTPVLVVSH"
BASE COUNT          655 a          379 c          433 g          581 t
ORIGIN      
        1 gtatgactgc cggcagtgag ggtggaaata cctacatcat cacctccgaa tgtaaatgaa
       61 ccgcaattgt catccccacc ggccaatccg ccatcatcgt ccttcatttg cattgtgtat
      121 gttgcgcctg gatctaaagc aactccgagt gatattgtca ctgggggtga agtgtccaca
      181 aatgcatcag cctctgtatt tactccgagc gcagtgataa ttaagtaaag gtccggcttt
      241 tcagcttcaa tcaatcctgt gcaaccaaaa atggtgcaat catcacaacc actcgaaact
      301 atggtggcat attctaattg gtatccgaaa gtgtcgatag taatggtatg gcttaccacg
      361 taatcggttg gaacaccgcc aactgcagta taagtctgag ttggagggaa ttctgagctg
      421 cttgtaacac cattaccgaa atcccagcta taggtaaatc catcaacgcc gtcgctgtga
      481 atactggttt caaaatctac attgattggg tcacaaccat aagtgagtga agggatgata
      541 ccaccatctt caggaacagc cggtaaaact tctaaaaata tgctgtattc cgcagcctgg
      601 tcgccacccg ggtattctaa agttgcgatt aaaggaacag taacttcata ccaaccggga
      661 acaagagggg ttccgcaaac cctggcacat ccgtataggc ttacatccgg gtcataatct
      721 acaggcaggt cgcctaaatt agttgaaacg ttcattccgt aaggcattcc caccggttca
      781 agaatatggt aattcaaaaa ctccagggtg ccaacaacgt caacagtggt gtctgcctgc
      841 atcacaaagg tgatatcact ttcgtaaaac tcaccagctg tcgcaggtgg aagggtatca
      901 ggataaacac cgggtgaaat ccaggcgtag tcgatgctgc atgtgctgca aacttgtgaa
      961 aagctttggt aggaaagcaa actaacaaat aaaaataata gggtaaaggt tttttgcatg
     1021 tgcgatacgg attttgatgg aaaattaggg attttttagg aatcatggtt tgggtggaat
     1081 ttttaaatgt tggtaaaaat ggtaatccaa cggcatttgc caattccttt gagtttatcc
     1141 acttgaagcc gcgtgttaaa cttaatttaa tatggttcta cactatcaac attattattt
     1201 gcttcacata aaacggaccg tgggcatggt gtcagatgta aacaaaaaat agaaaatcgt
     1261 gtacatttat acagacgtac atgattatca attcattaat gctttaccaa tatcgaatta
     1321 atatttgtag gctctatttt aaattaaaac aatggttttg ttgtgttaat atagtgttgc
     1381 cataacattg tggtaatacc atgtattgaa ctttgtccca acagtttaat catggaaagg
     1441 aaaaattttc tcaggtcact tggattactt gcagcgggaa caatggttcc attaaaaagt
     1501 aaatcggttg ttacgattaa tgagccgctg ccaactaatc aacgattgat gcgcattgca
     1561 catttaaccg atgcgcatat tgcagataat aaaaatgcac gcaatggatt tataaaatgt
     1621 ttacatgcta ttcagcaaat ggaagataca ccggattata taattaatgg aggagatgca
     1681 attgaggatg ctttatataa tacaaaatcg aatgtcaata aacaatggga tttgtggcat
     1741 tccattttaa aaaatgaatg tagtattaaa gtaaataata ccataggtaa tcacgatatc
     1801 tggggattgt ataccgagaa aaaagacttt ttgtatggca aaaaatttgc gcaggaaaaa
     1861 ttgcagatgg aacataccta ttatagtgtt gatcaatgcg gatggcatat tattttttta
     1921 gacagtacac agaaaaaaag taatgggatg tggtatactg caaaactagg agaggagcaa
     1981 attgattggt tgtcccgcga tttggctaca acacctgcaa atacacctgt tttggttgtt
     2041 tcgcatat
//
LOCUS       DAORKW010000630         2041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3482_length_2041_cov_3.763343, whole genome shotgun sequence.
ACCESSION   DAORKW010000630 DAORKW010000000
VERSION     DAORKW010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3482_length_2041_cov_3.763343"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLB46_16295"
     CDS             <1..316
                     /locus_tag="PLB46_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRF78140.1"
                     /translation="ICPCSMGTLGRIATGISNDLITRAADVMLKERRKLILVARETPY
                     NLIHIKNMETLTLAGGIVCPATPSFYSKPETFEQLAETVINRVMDLAGFDVGGYRWSE
                     NK"
     gene            378..1589
                     /locus_tag="PLB46_16300"
     CDS             378..1589
                     /locus_tag="PLB46_16300"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF78141.1"
                     /translation="MLAKFFRTYKEAYHGITKPVWILATIQLINRTGSMVLPFMTVYM
                     TSSLGYTKTEAGIIMSIFGIGSLAGSYLGGRWTDKFGPFKVQLLSLTIGGLSYLFIPF
                     LHSFYPLAIGVFICGLINDSLRPAASSMTTHFATEATTTRSFSLMRMAINLGVAIGPA
                     IAGLLAGINYMWLFIGDGATCIAAGIVFYYYFKDKQPQIHTEKKHATAPQISPYRDGK
                     FMLFMLFCFFYAAAFFQIFSGIPLYYKDVYFKPEAAIGLLIGFNGLIVFIFEMITVSQ
                     LEKRFRPNAMIVVGCVMLGLSFLIFNVFHGTIILLIAMLLLSFSEIFAMPFMISRVVK
                     SATPQTRGSYLAAYTIAWALAFIISPYGSTQIIENFNYTALWWIMGIFCLLTAFGFNA
                     IAQKQKATENI"
     gene            complement(1586..>2041)
                     /locus_tag="PLB46_16305"
     CDS             complement(1586..>2041)
                     /locus_tag="PLB46_16305"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78142.1"
                     /translation="DPEKSIIIRMFRKIFPVHNDYAGSSFFITKNGKKHATLLFIVLL
                     FIEFTDLIFAVDSIPAILAITPDPFIVYTSNVFAILGLRSLYFALSGIMHRFKYLIYG
                     LAAVLIFVGVKMCLVDVYKMPIGISLGVIAFVLTVSIVYSMYRTKNTDH"
BASE COUNT          627 a          383 c          379 g          652 t
ORIGIN      
        1 tatctgcccc tgctctatgg gaactcttgg tcgcatagca acaggtattt caaacgacct
       61 gattacccga gcagccgatg ttatgcttaa agaaaggcgc aaactcattt tagtagcaag
      121 agaaacgccc tacaatctga ttcatatcaa aaatatggaa accctgacgc tggcaggcgg
      181 cattgtttgt cctgcaacac catcatttta cagcaaacct gaaacttttg aacaattggc
      241 cgaaaccgtc ataaacaggg taatggacct tgccgggttt gatgtgggcg gttatagatg
      301 gagtgaaaac aaataataca ctgcagataa tcacagcggt tatttttcca aaaaaatgca
      361 tctcgtatct tcgcgctgtg ctagctaaat ttttcaggac atacaaagag gcttatcacg
      421 gaattacaaa accagtgtgg atattggcaa ctattcagtt gatcaaccgc acagggagta
      481 tggtattacc atttatgaca gtgtacatga ccagttcttt aggttataca aaaactgaag
      541 ccggtattat tatgagtatt tttggtatag gcagtttagc aggttcttat ttaggaggac
      601 gttggaccga taaatttggt ccttttaaag ttcagttgct gagtttaacc attggcggat
      661 tgagttattt atttattcca tttctgcaca gcttttatcc gcttgcaatt ggcgtattta
      721 tttgcggttt aattaacgat agtttgcgac ctgctgcatc atcaatgacg acacattttg
      781 caactgaagc aactacaaca cgttcgtttt cattaatgcg aatggctatt aatctcggtg
      841 ttgccattgg tcctgctatt gccggattat tagcaggtat taattatatg tggttattta
      901 ttggagatgg tgctacctgt attgcagcag gcatcgtttt ttattattac tttaaagata
      961 aacaaccaca aatccataca gagaaaaaac acgcaacagc accacaaatt tccccctacc
     1021 gtgacgggaa atttatgtta tttatgttgt tctgcttttt ttatgctgcc gcttttttcc
     1081 aaatatttag tggtattcca ctctattata aagatgttta ttttaaaccc gaagccgcaa
     1141 taggactatt aatcggcttt aacggattga ttgtatttat tttcgaaatg attaccgttt
     1201 cacaattgga aaaacgtttt cgaccaaatg ccatgatagt tgttggttgt gtgatgttag
     1261 gtttgtcgtt tctgatattt aatgtatttc atggcaccat tattttgtta attgccatgt
     1321 tgctactcag tttttcagaa atatttgcca tgccttttat gatttcacgt gtggtaaaaa
     1381 gcgcaactcc acaaacgcgc ggaagttacc ttgcagctta taccattgcc tgggcattag
     1441 cgtttattat ttcaccatac ggcagcacac aaattatcga aaatttcaac tacaccgcac
     1501 tttggtggat aatgggtata ttttgtttgc tgactgcctt tggatttaat gccattgctc
     1561 aaaaacaaaa agcaactgaa aatatttaat ggtcggtatt ttttgttcga tacatactgt
     1621 aaacaattga aacagtcagc acaaatgcaa ttacacctaa tgaaatgcca attggcattt
     1681 tatatacatc aaccaaacac atttttaccc caacaaaaat taatacagcc gccaaaccat
     1741 aaataaggta tttaaatcgg tgcataattc cggacaacgc aaaatacaaa ctacgtaaac
     1801 ctaatatcgc aaatacgttg gatgtataaa caataaatgg gtcaggcgtt atagctagaa
     1861 tagccggtat actgtctacg gcaaatatca ggtcggtaaa ttcaataaat aacaaaacaa
     1921 taaataataa ggtagcatgt tttttaccat ttttggtaat gaaaaaacta ctgccggcat
     1981 agtcgttatg caccggaaat attttcctga acatgcgaat aataatactt ttttcagggt
     2041 c
//
LOCUS       DAORKW010000631         2033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3506_length_2033_cov_5.354904, whole genome shotgun sequence.
ACCESSION   DAORKW010000631 DAORKW010000000
VERSION     DAORKW010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3506_length_2033_cov_5.354904"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLB46_16310"
     CDS             <1..292
                     /locus_tag="PLB46_16310"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF78143.1"
                     /translation="LFGPVASVITVKDADEAIVMANSHRYGLGATIFSRDTDRAYHFA
                     RQISSGAVFINAMVKSDPRLPFGGVKKSGYGRELADYGLKEFTNVKTIYIND"
     gene            323..1639
                     /locus_tag="PLB46_16315"
     CDS             323..1639
                     /locus_tag="PLB46_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF78144.1"
                     /translation="MQSTLSQTSALYEKRKQLVPNALGIFNPSSISKASGAIITDADG
                     KSFIDFAGGIGVINAGHCPEPVVKAIQAQSEKFIHACFNVSIYEQYLHAAEKMIDILP
                     HGEHTKIMFTNSGAEAVENAIKIARQATKREAVICYSQAFHGRTLLAATLTSKVGYKT
                     GFGPYAPEIYRIDFPAYDYYEGELNEDAFSMEHIEKLKAFFHHYVPAQNVAAIIVEPV
                     QGEGGFHVIPKKYLQELRKICTDNGIMLILDEVQTGFGRTGEWAAFHHYNVVPDLSTW
                     AKSMGSGMPVGAVIGKAEVMDNINPGTIGGTYLGNALCCVAVSATIDYMHSIDINAKG
                     KGVGAIIKNRFQQLQQKFPNHISRVRGLGAMMAFELSVNGNIHLPDSALTKKLAQRCY
                     ENGLIVLTAGVHGNVIRNLAPLVISETDLNRGLDIIENCLAEIIAA"
     gene            1649..>2033
                     /locus_tag="PLB46_16320"
     CDS             1649..>2033
                     /locus_tag="PLB46_16320"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRF78145.1"
                     /translation="MKKQYINGNYCDAENGGLWEVINPATEEIITTVPFGDDRDCLLA
                     IDAATHAFTTWKKISPWTRADILRKAADIIRKNISVFAQDMVMESGKPLAEAKGELTV
                     AANLFEWYAEEAKRGYGKVIPSSRTD"
BASE COUNT          682 a          333 c          437 g          581 t
ORIGIN      
        1 attgtttgga cctgtggcaa gtgtaatcac agttaaagat gctgatgaag caattgttat
       61 ggccaacagt catcgttatg gattaggtgc aacaattttt tcacgtgata cagaccgcgc
      121 atatcatttc gcacgacaaa taagcagtgg agcagtattt atcaatgcta tggtaaaatc
      181 ggatcctcgt ttgccgtttg gtggagtgaa gaaatccggt tatggtcgtg aactggcaga
      241 ttatggatta aaagaattta ccaatgtgaa aaccatttat ataaacgatt aaataaaata
      301 attttcagtg taaatagcta ctatgcaatc aaccttatca caaacatctg ctttatacga
      361 aaaacgaaaa caattagtgc ctaatgcctt gggcattttt aatccttcca gtataagtaa
      421 agcaagcgga gccatcataa cagatgcaga tggaaaatca tttatcgatt ttgctggagg
      481 aattggtgta attaatgccg gtcattgtcc cgaacccgtt gttaaagcta ttcaggcaca
      541 aagtgaaaaa tttattcatg cctgttttaa tgtttctata tatgagcaat atttacatgc
      601 tgctgaaaaa atgattgata ttttaccgca tggagaacat actaaaatta tgtttaccaa
      661 tagcggagct gaagcagtgg aaaatgctat taaaattgct cgacaggcaa ccaaacgaga
      721 agcggttatt tgttattcgc aggcttttca cggacgaaca ttattagcgg ctacgcttac
      781 gagcaaagtt ggttataaaa ccggtttcgg accttatgca ccagaaattt atcgcatcga
      841 ttttccggct tatgattatt atgaaggtga attaaatgaa gatgcatttt cgatggaaca
      901 tattgaaaaa ttaaaagcat ttttccatca ttatgtgcct gcacaaaatg tagcagcaat
      961 tattgtagaa ccagttcagg gtgaaggagg atttcatgtt attccaaaaa aatatttaca
     1021 ggaattaaga aaaatttgta cggataatgg catcatgtta attttagatg aggtgcaaac
     1081 cggctttggt agaaccggcg aatgggctgc atttcaccat tataatgttg tacccgattt
     1141 gtctacatgg gcaaaaagta tgggaagcgg aatgccggtt ggagctgtga taggaaaagc
     1201 agaagtaatg gacaatatta atcccggtac tattggtgga acatatttag gcaatgcttt
     1261 atgctgtgtt gcggtaagtg ctacaataga ttatatgcac tctattgaca tcaatgcgaa
     1321 gggaaaaggc gttggggcaa taattaaaaa cagattccaa caactccagc aaaaatttcc
     1381 aaaccatatt agtcgcgtaa ggggattagg tgcaatgatg gcatttgaat tatccgtaaa
     1441 tggcaacata catctgccgg attcggcgct taccaaaaaa ttagcacaac gttgttatga
     1501 aaatggatta atagttttaa ctgcaggtgt acatggtaac gtaattcgca atcttgcacc
     1561 gcttgtcatc agtgaaacag atttgaatcg cggcttagat atcatcgaaa attgtttggc
     1621 tgaaattata gctgcttaaa ttagatatat gaaaaaacag tacatcaatg gaaattattg
     1681 tgatgccgaa aacggtggac tttgggaagt aattaatccg gctactgaag aaattattac
     1741 cacggtacca tttggagatg accgtgattg tttgttagct attgatgctg caacacatgc
     1801 ctttaccacc tggaaaaaaa tatcgccatg gacacgtgca gatatactga gaaaagctgc
     1861 tgacatcatc agaaaaaata tttcggtatt tgcacaagat atggtaatgg aaagtggcaa
     1921 accattggca gaagcaaagg gcgaattaac tgtagcagca aatttatttg aatggtacgc
     1981 tgaagaagca aaacgcggat acgggaaagt aattccatct tccagaaccg ata
//
LOCUS       DAORKW010000632         2032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3510_length_2032_cov_4.423369, whole genome shotgun sequence.
ACCESSION   DAORKW010000632 DAORKW010000000
VERSION     DAORKW010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3510_length_2032_cov_4.423369"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLB46_16325"
     CDS             complement(<1..764)
                     /locus_tag="PLB46_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78146.1"
                     /translation="MLTTESKAQHALNLPTSINPTFLCDFQIEEIFLFENGSGAVYEP
                     VDSTLYKYDVWKRPERMERYFYDSLVAESKLEYDRVNKYTRITSLELDTFYVYNSCGL
                     IAEKYISTGSSDPKKASFSYRYSYDADNKLINRTLWDLKNYDTDFMLEEIVYSYNING
                     QVEEINTSLWLESEQLYTNRIKTIHYHPDMRDKVEKVYMDNFSPIADIPDSTMIFNYD
                     EHGKITSSFKLIPNNGTFDTIQATIFCYSIDNLLHTV"
     gene            complement(1013..1342)
                     /locus_tag="PLB46_16330"
     CDS             complement(1013..1342)
                     /locus_tag="PLB46_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78147.1"
                     /translation="MKKLMLIIALVVAGSLIASANNNNNLVPVYGIENGDQISGVGTM
                     MLKGDIWVIRVTDNEGVVDYCPMNLPQNFQKAGLIVKFSGTVKYISDTDRLAGMPLIL
                     SQIKLAR"
     gene            complement(1512..1814)
                     /locus_tag="PLB46_16335"
     CDS             complement(1512..1814)
                     /locus_tag="PLB46_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78148.1"
                     /translation="MEFKIVQDKGAYKQIDFGAGRSTQGFYQIAKKFEQILDISYSKK
                     SDNFNTLCWNYSYHGVPFQLTYHWDNGTLIKLQNQAPSKEEETAIDEIITVLSSYL"
BASE COUNT          693 a          347 c          317 g          675 t
ORIGIN      
        1 acagtatgaa gtaaattgtc aatactataa caaaagatcg ttgcctgtat agtgtcaaaa
       61 gtgccgttat tgggaataag tttaaaacta ctggtaattt taccgtgctc atcataatta
      121 aatatcatgg tgctatcagg gatatctgct atgggcgaaa agttatccat atataccttt
      181 tctactttat cgcgcatatc tggatggtaa tgaatagttt ttattcggtt ggtatataat
      241 tgttctgatt ccagccataa agatgtattt atttcctcta cttgaccatt gatattgtag
      301 ctataaacaa tttcttccag catgaaatca gtgtcataat tttttaaatc ccataatgtt
      361 ctgttaatta atttattgtc tgcatcataa ctatacctgt aactgaaact tgcctttttg
      421 gggtccgatg aacctgtaga gatatatttt tcagcgatta gtccgcagct attgtacaca
      481 taaaatgtgt ccagttccaa agatgtaatt ctggtgtatt tattaaccct gtcgtattcc
      541 aatttcgatt ctgcaactaa cgaatcataa aaatatcttt ccattctctc gggtcgtttc
      601 caaacatcat atttatataa agttgagtca accggctcat aaactgctcc actcccattt
      661 tcaaataaaa aaatttcctc aatttggaag tcacataaaa aggttggatt tattgaagtt
      721 ggtaaattta aagcatgttg agctttactt tctgttgtta acaataaagt aattgcaatt
      781 atgagtagac aagtatacct aactagcacg cataaaacta aacagaataa aataacaatt
      841 caaaaaaaaa ctgctgatta ccattcagtt ttgtattctt tgtgacaggg atttcaaata
      901 attgaggaag gtgccctgtt ttaaaaatag tgacaaagac aaattttcgt aataaaaaaa
      961 gaggctgtcg cacactgaca gcctctgatt accaaaatcc acatgattaa aattatcttg
     1021 ccaatttgat ttgtgacagt ataagtggca taccggcaag tctatcggta tcagaaatgt
     1081 attttacagt tcctgaaaat tttacaatta aaccggcttt ttgaaagttt tgaggaagat
     1141 tcattggaca ataatcaaca acaccttcat tatcagtaac tctaattacc catatatctc
     1201 cttttaacat catagttcct acaccggaaa tttgatcgcc attttcaatt ccatatacag
     1261 gaaccaaatt attattattg tttgctgaag caattaaact gcctgcaaca acgagcgcga
     1321 ttattaacat tagctttttc atatatctta gtttaagtag tgatgattaa tttttatgat
     1381 acaaaattcc atcacattaa taacctaaat tatgacttat gttaccaatc aatatgagaa
     1441 tagtcaagca ccctcaaaac gcctgtaata caggagtttc agcggaaaaa aagaataaat
     1501 ccagtatgtt tttataaata cgaactcaaa acagtgatga tttcatcgat ggcagtttcc
     1561 tcttctttac taggtgcctg gttttgtagt tttattaaag tgccgttatc ccagtgataa
     1621 gtcaactgaa atggaacacc atggtaagaa tagttccagc acaaagtatt gaagttatcg
     1681 cttttttttg aataggaaat atccaaaatt tgttcaaatt ttttggcaat ttgataaaag
     1741 ccttgtgtac ttctgcctgc tccaaaatca atttgtttgt aagcaccttt gtcctgcact
     1801 attttaaatt ccaaaatgaa gatttaatgg tgaatagaat gcattgaatc gatgaaaatc
     1861 agtgatggaa aattcaggta gaacaaccgt tacggaagaa cacttgattc aataaagtat
     1921 aaatataggc aaaaaaaacg acacccctag aaaaaaagat gaaacttgtg ctaaaagtat
     1981 aacaaaatga ctctaatatt tgagctgtat accccttccg gattgcgttt cc
//
LOCUS       DAORKW010000633         2028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3525_length_2028_cov_3.770400, whole genome shotgun sequence.
ACCESSION   DAORKW010000633 DAORKW010000000
VERSION     DAORKW010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3525_length_2028_cov_3.770400"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLB46_16340"
     CDS             complement(<1..382)
                     /locus_tag="PLB46_16340"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HRF78149.1"
                     /translation="MSLFKDFTLGIEEEFQIIDPETRDLSHRKHHIIENGAKFDVDIK
                     AEMHQSVVEVATSICKDMKDARNQVVKLRRIIAELAEKDNLKIAAAGTHPFASWEEQL
                     ITNHPRYQEIVNEMQEAARSNLIFG"
     gene            complement(401..1396)
                     /locus_tag="PLB46_16345"
     CDS             complement(401..1396)
                     /locus_tag="PLB46_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78150.1"
                     /translation="MKKIGIIHGMEDSFPTAFIDRINSKNIPGIVAERVTIDKVIQGE
                     ACGFDVLLDRISHDAPFYRSYLKNAAISGTAVMNNPFWWSADEKFFNNALAVKIGVPV
                     PNTVLLPSHDLPKNTKSTSFRNLKYPLDWESIFSYIGFPAYMKPHDGGGWRGVFKVDN
                     MEEFFSKYAETGDDVNILQSEVTFEYYFRCYCLGGKYVHIMYYEPRNPHHLRYTWNGP
                     EAPAELIEKVRVLTLALNQALGYDFNTVEWAVKDGIPYAIDFCNPAPDAERTSVGEEN
                     FEWVVEHAALMAIERAMEHKEGQDNLTWGTYVRNSVSGVAHKAETQPIFNPAVSR"
     gene            complement(1419..>2028)
                     /locus_tag="PLB46_16350"
     CDS             complement(1419..>2028)
                     /locus_tag="PLB46_16350"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRF78151.1"
                     /translation="NENRDFKLIESIGWFIESGIIKVYCPDSIDKHSWYNKQASAPDR
                     VLNHILYDRMLTEELFPVIQSETGYSRIGAAGCSFGGYHALNFAFRHPDIVAVMISLH
                     AKFDITGQLDGHYDDNVYFNNPPDYLPGLNDENLRKMQIYLGSAEFDMCLDANYQMAH
                     MLGMKNIPHTLEVVPGEKHDWPCWRQQLPRFLSLINYDTINS"
BASE COUNT          633 a          424 c          333 g          638 t
ORIGIN      
        1 atccaaaaat taaattactg cgcgcagctt cctgcatttc attcacaatt tcctgataac
       61 gcggatggtt ggtaatgagt tgttcttccc acgatgcaaa aggatgtgtt ccagccgcag
      121 caatttttaa attatctttt tccgctaatt cagcaataat acgacgcaat ttcacaacct
      181 gattgcgggc atctttcatg tccttacaaa tgctggtagc tacttcaact acactttgat
      241 gcatttccgc ttttatatca acatcgaact ttgcaccatt ttcaataatg tgatgtttgc
      301 ggtgcgataa atcacgagtc tcagggtcga taatctgaaa ttcctcctca atacctaacg
      361 tgaaatcttt aaataaactc ataatcaaaa atatattgga ttaacgtgaa acagcaggat
      421 taaaaattgg ttgagtttct gctttatgcg ctacaccaga tactgaattg cgcacatagg
      481 ttccccaggt taaattatcc tggccttctt tatgttccat agcgcgttca attgccatta
      541 atgcagcatg ttcaaccacc cactcgaaat tctcctcacc aacagaagtg cgttctgcat
      601 ccggtgcagg attacaaaaa tcaattgcat atggaatacc atctttaact gcccattcca
      661 cagtattaaa atcataacct aacgcttgat ttaatgctaa ggttaatact cgtacttttt
      721 caattaattc agcaggtgct tcaggtccat tccaggtata acgcaaatga tgcggattgc
      781 gtggttcgta atacataata tgcacgtatt ttccaccaag gcaataacat ctgaaataat
      841 attcaaaagt aacttcgctt tgtaaaatat ttacatcgtc acctgtttct gcatatttgg
      901 agaaaaactc ttccatatta tccactttaa aaacacctct ccaaccaccg ccatcatgtg
      961 gtttcatata tgcaggaaat ccgatataac tgaaaataga ttcccagtcc aaaggatatt
     1021 ttaaattgcg gaatgaagtt gactttgtgt ttttaggtaa atcatgagat ggtaacaaaa
     1081 cggtatttgg aaccggaaca ccaattttaa ctgctaatgc attattaaaa aacttttcat
     1141 cagcactcca ccaaaatgga ttattcataa cagctgttcc gctaatggca gcatttttca
     1201 aataacttct gtaaaatggt gcatcatgtg aaatacggtc taataaaaca tcgaaaccac
     1261 atgcttcacc ttgtataact ttatcgatag taactcgttc agctactata ccaggaatgt
     1321 ttttactatt tattctatca ataaacgcgg ttggaaatga atcttccatg ccatgtataa
     1381 tcccaatttt tttcataagt gtagtatttt tttaaaggtt aagagttaat tgtatcgtaa
     1441 ttaatcaaag ataaaaatct tggcaattgt tgacgccaac atggccagtc gtgtttttct
     1501 cccggcacta cttcaagtgt gtgtggaatg tttttcatgc ctaacatatg ggccatttga
     1561 taattggcat ccaaacacat gtcgaattcg gcagaaccca ggtatatctg cattttccga
     1621 agattttcat catttaatcc cggcaaataa tccggtggat tgttgaagta cacattatca
     1681 tcataatgcc cgtctaattg tcccgtaata tcaaatttcg cgtgtaagga aatcattaca
     1741 gccactatat ccggatgtct gaaagcaaag tttaaagcgt ggtatccgcc aaaactgcaa
     1801 ccggcagcac caatacgtga ataacctgtt tctgactgaa taaccggaaa caattcctcc
     1861 gtcaacatac ggtcgtataa aatatgattt aaaacacggt caggagctga agcttgttta
     1921 ttataccaac tgtgtttatc aatgctgtct ggacaataca ccttaataat gccggattcg
     1981 ataaaccatc caatggattc aatcaattta aaatcgcggt tttcattg
//
LOCUS       DAORKW010000634         2022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3549_length_2022_cov_4.474326, whole genome shotgun sequence.
ACCESSION   DAORKW010000634 DAORKW010000000
VERSION     DAORKW010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3549_length_2022_cov_4.474326"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /gene="msrA"
                     /locus_tag="PLB46_16355"
     CDS             complement(<1..469)
                     /gene="msrA"
                     /locus_tag="PLB46_16355"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRF78152.1"
                     /translation="MLKTNIVILSLTVAVISMIGCGSGNPESQESKPEQIKVDYLSMR
                     KTYTDAEIQQNGWEKATFGGGCFWCTEAVFEEVKGVQSVLSGYSGGAMKNPTYEAVCT
                     DTTGHAECVQIIYDPKVCSYATLLDIHMKTHDPTTPNRQGNDVGIQYRSVVFYD"
     gene            661..1581
                     /locus_tag="PLB46_16360"
     CDS             661..1581
                     /locus_tag="PLB46_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HRF78153.1"
                     /translation="MRQIKIAVHGGAGTMPKRLMTPEIEKACHDALQNALQAGYDLMK
                     NGGSAFDAVEAAIIILENFELFNAGKGAVFTHDGKHEMDASIMDGKTLRSGAVAAVNN
                     IKNPISLAKAVMLHSEHVLLLGSGAEVFAKQQNIVFETDDYFFTQFRYDQLEKAKKSD
                     IIVLDHVDDKKFGTVGCVALDANGNLAAGTSTGGMTNKSFGRVGDSPIIGCGTYANNQ
                     ICAVSCTGHGEFFIENVVAYDVACLIDYKGLSLKEATELVINEKLKKINAEGGLIAID
                     KFGDISLPFNTKGMYRGYIKSDTDIFTGIY"
     gene            complement(1588..>2022)
                     /locus_tag="PLB46_16365"
     CDS             complement(1588..>2022)
                     /locus_tag="PLB46_16365"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HRF78154.1"
                     /translation="IAGTSAGAMAQSEKMINGGTPSEAVKRGKAMMMSGLGFIDNAII
                     DSHFVNRGRFGRLMVAVAEHPKLTGIGISEDTGVIIKEERHLEIIGSGEVVIIDGQEL
                     RYNSLDNKEYSGLNIEHMIFHLLSAGMFYDLKTRKLALQEQH"
BASE COUNT          627 a          366 c          386 g          643 t
ORIGIN      
        1 catcataaaa aactactgaa cgatattgaa taccaacatc attaccctga cggttaggag
       61 tagtcggatc atgggttttc atgtgaatat ccagcaatgt tgcataactg caaactttag
      121 ggtcgtatat aatttgtaca cattcagcat gaccggttgt atccgtacaa accgcttcat
      181 acgtagggtt tttcattgcg ccaccggaat agccgctcag cacagattga acacctttta
      241 cttcttcaaa aacggcttct gtacaccaga aacatccacc accaaaggtg gccttttccc
      301 atccattttg ctgaatttca gcgtctgtat atgtttttct catagataaa taatccactt
      361 ttatttgctc aggtttcgat tcttgagatt ccggattgcc ggaaccacag ccaatcatcg
      421 aaataactgc tactgtgagc gaaagtataa caatatttgt ctttaacatg tttatagttt
      481 aatcaactta cgcaaacata acacattcgg atgtatgtaa agtttcagga ttaacaaggt
      541 ttaacgattc aaagcgctca attatgaacc taagttttta ttgtaattac cataacaatt
      601 atgttagata ggaagtagga caagcaattt gtgttcatgg gtattttcta tatttgcatt
      661 atgcgccaaa ttaaaattgc cgttcacggt ggagcaggca caatgccaaa acgattaatg
      721 acccctgaaa tcgaaaaagc ctgccacgat gctttacaaa atgctttaca agccggatat
      781 gatttgatga aaaatggagg ttctgcattt gatgctgttg aagcagccat tatcatatta
      841 gaaaattttg aattgtttaa tgcaggtaag ggtgcagtat ttacgcatga tggcaaacat
      901 gaaatggatg ccagtataat ggatggcaaa actttgcgta gtggtgctgt tgctgctgta
      961 aataatatta aaaaccctat ttcattggca aaggctgtta tgttacattc tgaacatgta
     1021 ttgttgttgg gttcaggtgc ggaagttttt gcaaaacaac aaaatattgt ttttgaaacc
     1081 gatgattatt tttttacaca atttagatat gatcaattag agaaagcaaa aaagagtgat
     1141 ataattgttt tggaccatgt agatgataaa aaatttggga cagttggatg tgttgcctta
     1201 gatgctaatg gaaaccttgc tgcaggaact tcaactggcg gaatgaccaa taaatcgttt
     1261 ggacgtgttg gtgattcacc tataattggt tgtggaacct atgcgaataa tcaaatttgt
     1321 gcagttagct gcaccggaca cggtgaattt tttattgaaa atgttgtggc ctatgatgtt
     1381 gcctgtttaa ttgattataa aggcctcagc ttaaaagaag caactgaatt ggttatcaat
     1441 gaaaaactga aaaaaattaa tgccgaaggt ggattaattg ccattgataa attcggagat
     1501 attagcttgc catttaatac taagggaatg tatcgtggtt atattaaaag cgatacagat
     1561 atatttaccg gcatttatta aaagcaatta atgttgttcc tgcaacgcta atttcctcgt
     1621 ttttaaatca taaaacatac ctgcactcaa taaatgaaat atcatatgtt cgatatttaa
     1681 tccggaatat tccttattgt cgagtgaatt gtaacgcaat tcctgaccgt cgattataac
     1741 aacttctccg ctgccaatta tttcgagatg acgttcttct ttaataatta caccggtatc
     1801 ttcgctgata cctattcctg ttaatttcgg atgttcggca acagccacca ttaaacggcc
     1861 aaagcgaccg cggttgacaa aatgagaatc gattatagca ttatcaataa atcccaaacc
     1921 cgacatcatc attgcttttc cacgttttac agcttccgat ggggtgccgc cgttaatcat
     1981 tttttcgctt tgagccatag cacctgcgct ggttcctgca at
//
LOCUS       DAORKW010000635         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3556_length_2020_cov_4.782697, whole genome shotgun sequence.
ACCESSION   DAORKW010000635 DAORKW010000000
VERSION     DAORKW010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3556_length_2020_cov_4.782697"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..691
                     /locus_tag="PLB46_16370"
     CDS             101..691
                     /locus_tag="PLB46_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HRF78155.1"
                     /translation="MGSKIEQIKGVIAESIRVKNEILNNPEMIAKCEEIAMKIADAFR
                     QDKKVLFCGNGGSAADAQHLAAEFSGRFYFDRPPLDAEALHVNSSYVTAVGNDYGYDQ
                     VFSRVLKAKGRQGDFIFGISTSGNSENILKAFEVARERGMIIVAMTGASGGKMKDNCD
                     YLINVPSSDTPRIQESHIMLGHIICELVEAELFERP"
     gene            688..1383
                     /locus_tag="PLB46_16375"
     CDS             688..1383
                     /locus_tag="PLB46_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRF78156.1"
                     /translation="MIREAIVLAGGFGTRLQTVVKEVPKPMAPVAGKPFLQYILDYLI
                     AHKVAHVVLAVGYLRETIIDYFGDNYQSLSITYAVEENPLGTGGGILNACNQIKGDNV
                     FVINGDTFFDVDLVELSAFHESNNALLTVALKKMEKFDRYGTVETDNEGRIIGFLEKK
                     YLDEGLINGGIYCLNKHIFHPELPAAFSFEKEILEKEIVNRKIYGLRSEGYFIDIGIP
                     EDYARAQDDFKGK"
     gene            1393..1929
                     /locus_tag="PLB46_16380"
     CDS             1393..1929
                     /locus_tag="PLB46_16380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HRF78157.1"
                     /translation="MENKLPQIDTSWTLFLDRDGVLNKKIDDDYVRNWQQFEFLPGVK
                     SALAICAEKFGRIVIVTNQRGVGRGLMSETDLHEIHMQLMDEVEAAGGRIDAIFYCPD
                     ITDEGSTHRKPQPGMGYDAQHLFPEIDFAKSIIVGDSGSDMAFGKNLHMFTVLIAQNP
                     KPNADLVLPSLMRFAELL"
BASE COUNT          614 a          363 c          428 g          615 t
ORIGIN      
        1 ttgaaacttc tcgaactacc tctctccaga gctttgaagt gctcaacaca tttggtggtg
       61 aagtacataa tttagaattc tcaaaatacg gattaacatc atgggtagca aaatagaaca
      121 aataaaaggc gtaatcgccg aaagtatcag ggtgaaaaat gaaatattga ataaccctga
      181 aatgattgct aaatgcgaag aaattgcaat gaaaattgca gatgcatttc gtcaggataa
      241 aaaagtatta ttctgtggta acggtggaag tgctgccgat gcacaacatt tagcagccga
      301 attttcaggt cgtttttatt tcgatcgccc accactggat gcagaagcat tacatgtgaa
      361 ttcatcctat gtaactgcag ttggtaacga ttatggttac gaccaggttt tttcacgtgt
      421 attaaaagcg aaaggtcgcc agggtgattt tatttttggt atttctactt caggaaattc
      481 tgaaaatatt ttaaaagcat ttgaagttgc tcgtgaacgc ggtatgatta tcgttgccat
      541 gacaggtgct tcgggtggta aaatgaaaga caactgcgat tatttaatca acgtgccttc
      601 aagcgataca cctcgtattc aggaaagtca tattatgctc ggccatatca tctgcgaatt
      661 agtagaagcc gaattatttg aacgtccatg attcgtgaag cgatagtatt agccggtgga
      721 ttcggaacac gtttgcaaac cgttgtaaaa gaggttccta aaccaatggc tcccgttgcc
      781 ggtaaaccgt ttttgcaata tattctcgac taccttattg cacacaaggt agctcatgta
      841 gtgcttgctg tcggctattt acgcgaaaca attatcgatt attttggcga taattatcaa
      901 tcgctttcca taacttatgc cgttgaagaa aatccacttg gtacaggtgg cggaatttta
      961 aatgcctgca atcaaattaa aggcgataat gtttttgtga ttaatggcga tacttttttt
     1021 gatgttgatt tagttgaact ttctgcattc catgaatcga ataatgcatt actcactgtc
     1081 gcattaaaaa aaatggaaaa attcgaccgt tatggcactg ttgaaacaga taatgaaggg
     1141 agaataattg gatttctgga aaaaaaatat ctcgacgaag gattgattaa tggcggtatt
     1201 tattgcctga ataaacatat ttttcatccg gaattaccag cggctttttc gttcgaaaaa
     1261 gaaattcttg aaaaagaaat tgtcaataga aaaatttacg gacttcgttc agaaggatat
     1321 tttattgaca taggcattcc cgaagattac gcacgtgctc aggacgattt taaaggtaaa
     1381 taagtattgg tcatggaaaa taaattaccg caaatagata cttcatggac tttatttctc
     1441 gaccgggatg gcgtgctcaa taaaaaaatt gacgatgatt atgtccgcaa ttggcaacaa
     1501 tttgaatttt tgccgggtgt aaaatctgca cttgccattt gtgcggaaaa atttggcaga
     1561 attgtaattg tcaccaatca gcgtggtgtt ggcagaggat tgatgtccga aaccgacttg
     1621 catgaaatac acatgcagtt aatggatgag gtggaagcag ccggggggcg tatagatgcc
     1681 attttttatt gccctgatat taccgatgaa ggctccacac atcgcaaacc acagccgggt
     1741 atgggttatg atgcgcaaca tctgttccct gaaatcgact ttgccaaatc aattattgta
     1801 ggtgattcgg gcagcgatat ggcctttggc aaaaacctcc acatgttcac cgttttgatt
     1861 gcccaaaatc ctaaaccaaa tgccgatttg gtattgcctt cgctgatgcg ctttgcagag
     1921 ctgctctgac cgctattttg gccactcttt tagccgtttt caggcaaatt gtgcattaaa
     1981 taaatatcat tttttttaac ctgagttaac tctttcatag
//
LOCUS       DAORKW010000636         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3559_length_2020_cov_3.969466, whole genome shotgun sequence.
ACCESSION   DAORKW010000636 DAORKW010000000
VERSION     DAORKW010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3559_length_2020_cov_3.969466"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PLB46_16385"
     CDS             complement(<1..674)
                     /locus_tag="PLB46_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78158.1"
                     /translation="MKLGNTLKNIIIGCSGVLFMQGCEIINPEEKIPSYVYINEFSFT
                     TSLDIQGYASSKLPDAWVYANNEFIGAFELPATVPILADGETEIIVYPGIKENGISGT
                     GMIYPFYNPYTITRNLIAGATDTISPATTYKNSAAITFLMIERFESSNPFYSVIEDAI
                     LTTTSDESLVFEGNRSAISTMTGAIDTFYVQTSDPLFFPAIDHQLFLELDYKCDIPFN
                     IWVKCNT"
     gene            complement(703..1686)
                     /locus_tag="PLB46_16390"
     CDS             complement(703..1686)
                     /locus_tag="PLB46_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRF78159.1"
                     /translation="MVRIGVLGAGHLGKIHLKLLKEIPDFELVGFYDPDETNAKYAAE
                     TFQIPRFNSIHKLIEVCDAVDIVTPTISHYDCARAALKKAKHIFIEKPLANTVSEAKY
                     IMSLAHEANVKAQVGHVERFNPAFLAIKDLDIQPMFIESHRLSTFNPRGTDVSVVLDL
                     MIHDIDIVLHLVHSNVKKVSASGVKVLSNQPDIANARLEFDNGCVANLTASRISMKKM
                     RKMRLFQRDAYIAVDFGENMTEILTMSDANPDEDAFTIEINTGDASKKRVIKMDKPVI
                     TPVNAIKMELEQFAKAIINDTDTPVTIEDGYRALQVAHMILHKIEKNVAYR"
     gene            1804..>2020
                     /locus_tag="PLB46_16395"
     CDS             1804..>2020
                     /locus_tag="PLB46_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78160.1"
                     /translation="MKNALSSPRNSGMTKSSFSLPLVLLPLLSGLLLWAAWPPLPYTF
                     LVFIGFVPLLLGAKMVNDRFVKFTSFKV"
BASE COUNT          605 a          391 c          358 g          666 t
ORIGIN      
        1 gtattacatt ttacccagat attgaaagga atatcgcatt tataatctaa ctctaaaaat
       61 aactgatggt caatagccgg aaaaaataat gggtcgctgg tttgaacata aaatgtatca
      121 attgcacctg tcatagtgct tatagctgag cgattgccct caaacaccaa agattcatca
      181 gaagtagtag ttagtatagc atcttcaatt acactataaa acgggttgct gctttcaaac
      241 cgttctatca taagaaatgt gattgcagca gaatttttat aggttgttgc aggcgatata
      301 gtatctgtag ctccagcaat taaatttctt gttatggtat aaggattata aaatggataa
      361 atcattccag ttcctgatat tccattttcc tttattccgg ggtaaacaat tatttctgtt
      421 tcaccatcgg caagaattgg aacggtagca ggtaattcaa atgccccaat aaattcgtta
      481 ttcgcataca cccaggcatc aggtaatttt gatgaagcat aaccctgaat atctaaactg
      541 gttgtaaacg aaaattcgtt aatgtaaaca taagatggaa ttttttcttc ggggttgata
      601 atttcgcaac cctgcataaa tagcacccca ctacagccaa ttatgatatt ttttaatgta
      661 tttcccagct tcatcaataa ttaattttta aatgcttttc gattaccgat aagcaacatt
      721 tttttcaatt ttatgcagaa tcatatgggc aacttgtaaa gcgcgataac catcttcaat
      781 agtaaccggt gtgtcagtat cgttgataat agctttggca aattgttcta attccatttt
      841 tatcgcatta acgggagtaa ttacaggttt atccatttta attactcgtt ttttggatgc
      901 atcaccggtg ttaatctcta ttgtaaacgc atcttcatcc ggattagcat cactcatcgt
      961 taatatttca gtcatatttt ccccaaaatc tactgcaata taggcatcgc gttgaaaaag
     1021 acgcatttta cgcatttttt tcatggaaat acgacttgca gtaagattag caacacaacc
     1081 gttgtcaaat tcaagacggg cattagctat atcaggttgg ttactcaata ctttcacacc
     1141 acttgcactc acttttttga cattagaatg aacaagatgc agcacaatgt cgatatcgtg
     1201 aatcatcagg tccaatacca ccgaaacatc tgttcctctt ggattaaatg tagaaagacg
     1261 gtgcgactca ataaacatgg gctgaatatc aaggtccttg atggctaaaa aggcagggtt
     1321 gaagcgctcc acatgcccca cttgtgcctt tacattggct tcatgagcaa gcgacatgat
     1381 gtatttggcc tcgctaaccg tattggctaa gggtttttca ataaaaatgt gtttggcctt
     1441 ttttaatgct gctctggcgc aatcgtaatg tgaaatggtg ggtgtcacaa tatcaacggc
     1501 atcgcatacc tcaataagtt tgtgaatgct gttgaaacgc ggaatctgga atgtttctgc
     1561 cgcatatttc gcatttgttt cgtccgggtc gtaaaagcca acaagctcaa agtcaggaat
     1621 ttccttcagt aacttcaaat ggattttccc caaatgtccg gcacctaata caccaattct
     1681 aaccatacat ggatttgtgg caaatttaat ttattcacct tgagcgttat ataaaatgaa
     1741 gatataaatg tccacgtttt atacaatatg tatatcaacc aacacagttt accaaaatct
     1801 gacatgaaaa atgctttatc ttcacctcga aattcaggta tgacaaaaag tagtttttct
     1861 ttaccactgg tattactacc cttgctttca ggtttattgc tctgggctgc ctggccgcct
     1921 ttaccttata cgtttttggt ttttatcggg tttgttccac tgcttctggg tgcaaaaatg
     1981 gttaatgaca ggtttgtaaa atttacttct ttcaaggttt
//
LOCUS       DAORKW010000637         2018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3566_length_2018_cov_4.191544, whole genome shotgun sequence.
ACCESSION   DAORKW010000637 DAORKW010000000
VERSION     DAORKW010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3566_length_2018_cov_4.191544"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="PLB46_16400"
     CDS             <1..1770
                     /locus_tag="PLB46_16400"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRF78161.1"
                     /translation="DDITIVLLIVGFTLFMFIWEKIAIDITSLIAMALLMLTGVLTTK
                     EGIAGFSNDATVTILFLFLLSAGLEKTGAVNLVGKAMIKYIGKNQKMAFIAVILVCGL
                     ISAFLNNTAVVIIFMPIVFKIAKFTNQSPSKLLMPLSFAAIMGGTLTLIGSSTNLVVN
                     GVVVEHGYEPFGMFEVTPTGLILFVTFFLYMYFIGIKLIPARRSADSLTEDYELKDFL
                     TEIIIEPGSPFLGKRIFDTPLVTELSLEVVEISDAKGTLWLPDDYELLEENDTVLVRG
                     SASDIMQLKQMQGVTFKQTFDVDDIDLKSNETALVEVVIGPNSSLARSTFKEIDFRDL
                     YEAIPLAVRRQGEIIEGRLDEIELRFGDDLLLEIKKDSFDQLKRGGDFVFTQEIEKEI
                     IDKQKVWITSGILLMVILTSAFEILPIIQGAIIGTILMFLFKCITIREAYREVDWRII
                     FVLAALLPLGTALEKTGAAEMLAGYMETYISGYGPFITLFVLFAVTSVITSLMSNQAT
                     AALLSPIAISLGVQMGIDPKPLLFAVMFAASTCYLTPLGYQTNIMIYGPGNYKFVDFI
                     KVGGLLSLISGIVVSLCLYYFYF"
     gene            1783..>2018
                     /locus_tag="PLB46_16405"
     CDS             1783..>2018
                     /locus_tag="PLB46_16405"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HRF78162.1"
                     /translation="MSSKTIYFLGDLFSFHHLAGIGFFGGDNNFIAAANFDDIINQTI
                     SQEGHFAVIAVENTLAGDVPGNFQRISYSGLTVIG"
BASE COUNT          580 a          300 c          418 g          720 t
ORIGIN      
        1 gatgatatta ctattgtatt gctcattgta ggatttacat tatttatgtt tatctgggag
       61 aaaattgcca tcgatattac atcgttaatt gcaatggcac ttttaatgct tacaggagtg
      121 ctaacaacta aagagggaat agccggattt agtaatgatg ccacggttac aattttattt
      181 ttatttctac ttagtgcagg gcttgaaaaa accggtgctg taaatctggt tggtaaagct
      241 atgattaaat atatcggcaa aaatcaaaaa atggcattta ttgctgtgat attggtatgt
      301 gggcttattt ctgcattttt gaataataca gccgttgtaa taatttttat gccgattgtg
      361 tttaaaatag ccaaattcac caatcaaagt ccgagcaaat tactcatgcc tttatcgttt
      421 gcggctataa tgggtggcac gttgacactt ataggaagtt ctacaaattt agttgtaaac
      481 ggtgtggtag tggaacatgg ttatgaacct tttggtatgt ttgaagttac accaacaggc
      541 ttaattttat ttgttacatt ttttctttac atgtatttta ttggtattaa attaatacct
      601 gcaagaaggt cggccgattc gctgactgag gattatgaat taaaagattt tttgacagaa
      661 ataattattg aaccgggttc tccattttta ggcaaacgta tatttgatac gcctttggtt
      721 acagaattat ctttagaagt ggttgaaata agtgatgcaa aaggcacgct gtggttgccc
      781 gatgattatg agttgcttga agaaaatgat acggtattgg ttcgtggtag tgcatctgat
      841 attatgcaat tgaaacaaat gcagggtgta acatttaaac aaacttttga tgttgatgat
      901 attgatttaa aatcgaatga aacagcattg gttgaagtgg taattggtcc taattcctcg
      961 cttgcaagaa gcacatttaa agaaatagat ttccgcgatt tatacgaagc catcccgctt
     1021 gctgtgcgcc gacaaggaga aattattgaa gggcgtttag atgaaattga attgcgtttt
     1081 ggtgatgatt tattattgga aatcaaaaaa gattcatttg accaattaaa acgtggtggt
     1141 gattttgtgt ttactcagga aatagaaaaa gaaatcatcg acaagcaaaa agtatggatt
     1201 accagtggca ttttattgat ggtaattctc acttccgctt ttgaaatttt gcccattatt
     1261 caaggcgcaa ttattggtac cattttaatg tttttattta aatgtattac cattcgtgaa
     1321 gcataccgtg aagtggactg gaggataata tttgtattgg cggcattact tcctttaggg
     1381 acagctcttg aaaaaaccgg tgcagctgaa atgctagctg gttatatgga aacatatatt
     1441 tccgggtatg gtccatttat cacacttttt gtgctgtttg ccgtgacttc cgttatcaca
     1501 agtttgatgt ccaatcaggc gactgctgca ttgctaagtc caattgctat ttcgcttggc
     1561 gtgcaaatgg gtattgaccc taaaccgctt ttatttgccg ttatgtttgc tgcttcaaca
     1621 tgttatctga cccctctagg gtatcagaca aatattatga tttatggccc gggtaattat
     1681 aaattcgttg attttataaa agttggtggt ttattgtcgc tcattagtgg tattgttgtt
     1741 tctttgtgtc tgtattactt ttatttttaa tgtaaccaca ttttgagcag taaaaccatt
     1801 tattttttag gcgatttatt ttcattccat catttagcag gaataggttt ttttggtgga
     1861 gataacaatt ttattgcggc tgccaatttt gatgatatca taaatcaaac aatatcacaa
     1921 gaaggccatt ttgccgtgat tgcagtagaa aatacacttg ccggtgatgt tccgggtaat
     1981 tttcagcgta tatcctattc agggctcact gtaattgg
//
LOCUS       DAORKW010000638         2017 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3570_length_2017_cov_3.498471, whole genome shotgun sequence.
ACCESSION   DAORKW010000638 DAORKW010000000
VERSION     DAORKW010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3570_length_2017_cov_3.498471"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="PLB46_16410"
     CDS             complement(<1..641)
                     /locus_tag="PLB46_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78163.1"
                     /translation="MKKILGIDLGTTSIGWALIQAEEDNKTLIDMGAHVIPYTERTEA
                     EEFGKGQGISKNQARTAARTSRKMLNRYQQRRSSIIKFLTHNNMLPEKEIYNLSPLAL
                     YKIRANAANQQVSLGEIGRLILLLNQRRGYKHATEDFSADNTNSEWVQNIDDRYGRIK
                     GKQTIGQFFYERLIEAKEGNKYYRIKEKIFPRAAYIEEFDKIWNTQALFYPEI"
     gene            1364..>2017
                     /locus_tag="PLB46_16415"
     CDS             1364..>2017
                     /locus_tag="PLB46_16415"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRF78164.1"
                     /translation="MENLSTKFLGESLTFDDLLVVPAYSEVLPREVDISTQLTRNLRI
                     NVPIISAAMDTVTEYKMAVAMAREGGIGIIHKNMSIEKQADQVRRVKRSDSGLIIDPV
                     TLSQNAIIRDALAMMKEFKIGGIPVVDENKKLVGILTNRDLRFETNMQRPIAEVMTTT
                     NLITAPEGTTLQQAENILKNYRIEKLPVVDGNGILKGLITYKDILKLASHPRACKDKY
                     "
BASE COUNT          585 a          378 c          367 g          687 t
ORIGIN      
        1 aatatttctg ggtaaaacag cgcttgtgta ttccatattt tatcaaactc ttctatatag
       61 gccgccctag gaaatatttt ttcttttatt ctataatatt tatttccttc tttagcctcg
      121 attaaacgtt cataaaaaaa ctgacctata gtttgttttc ctttaattct gccatatcta
      181 tcatcaatat tttgaaccca ttcactattt gtattatcag cagaaaaatc ttctgttgca
      241 tgtttataac ccctacgctg atttaacaat aatatcaatc ttccaatttc ccccaagcta
      301 acttgttggt ttgcagcgtt ggctctaatt ttataaaggg ctaaaggtga caaattatat
      361 atttcttttt cggggagcat attattatgt gttaaaaact taataataga actccttctt
      421 tgttgatatc tattaagcat ttttcttgag gtgcgagctg ctgtccttgc ttggtttttg
      481 ctaatgccct gtcctttccc aaattcttct gcttcagtgc gttcagtata tggtattaca
      541 tgagccccca tatcaataag agttttattg tcctcttcgg cctggattaa tgcccatcct
      601 attgatgttg ttcctaagtc aatacctaat atttttttca tatatttgta taattgattt
      661 atttgatata cttttgaatt ataatttgaa tcaattcaca ataaggattt tattccgttg
      721 tgattacatt tggagcggtg caagtcgctc tttttttata tcaaacttac acaataattt
      781 tccaaaatac ctcatttgat gttctaattc acggtaaacc gttaatgtca actgtgtcac
      841 aattcctaaa gatttatttg tgtttttaaa tgttaaaaat ctcgatttaa tatcacaaat
      901 taatttttat ttagacttag atgaaacaaa ttatacttaa cagctgtttg tggtcgtatg
      961 caattcgccc ctacatttta attcattagt ttagggcatt ttgagtatga ctttttattt
     1021 gggagtaaga aaatgttgaa tgtgtagggg cgaattgaat ccgcccctac acattcaaca
     1081 ttttcgaatt cggagctccg ctgcggagga cgagccaaga ttcatattta tttttcaaat
     1141 tgtcaattag aagataaatt ccatttttcg ctccgaatcg cgttagggat agtagcggaa
     1201 accccacagc cgaggaacga ggcgaggagt tgcagcggat agcccggccc ccctatagta
     1261 gattaattat ttgcgaaggg caaataatta atcactatag ggctgggcaa cgccagaaat
     1321 aaatgcattt ttgcaaattc aaattcccgt tctatctttg cacatggaaa atctctcaac
     1381 taagtttttg ggtgaaagtc tcaccttcga tgatctcctt gtagttccgg cctattctga
     1441 agtattacca cgtgaagtag atatttcaac tcaactcaca cgcaatttgc gtattaatgt
     1501 gcctattatt tcggctgcaa tggacactgt gactgagtat aagatggcag ttgccatggc
     1561 cagagagggt ggtattggta tcattcacaa aaacatgagt attgagaagc aagcagacca
     1621 ggtaagacga gtgaaaagga gtgatagtgg actgattatt gaccctgtta ccttgtcaca
     1681 aaacgccatt attcgcgatg cacttgccat gatgaaggaa ttcaaaattg gtggcattcc
     1741 ggttgttgat gaaaataaga agctggttgg gattttgacg aaccgcgatt tgcgttttga
     1801 aaccaatatg caacgcccga ttgccgaggt gatgacgacc acgaatttaa ttactgcgcc
     1861 tgaaggaaca accttgcaac aggccgaaaa tattctcaaa aattaccgta tcgaaaagct
     1921 tcctgttgtt gatggcaatg gcattttgaa gggattgatt acctacaaag acatcctcaa
     1981 actagcgagt cacccacgcg cttgtaaaga taaatat
//
LOCUS       DAORKW010000639         2005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3602_length_2005_cov_4.552821, whole genome shotgun sequence.
ACCESSION   DAORKW010000639 DAORKW010000000
VERSION     DAORKW010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3602_length_2005_cov_4.552821"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PLB46_16420"
     CDS             complement(<1..1116)
                     /locus_tag="PLB46_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78165.1"
                     /translation="MRIPYCIIVLVSFFCCIKFPCICQVPEIIWESPFGGNFDDRAQD
                     IVQDAEGNILTVGYSDSFDGDVSFNHGLIDFWAVMQNSDGEILWEKSYGGSSYEVCSS
                     VCLANDGGFILAGSTFSNDGDVTLNQGSTDCWVIKIDILGEIIWQKTYGGSSPDGIYS
                     IKQISTGGYIVAAQSMSQDGDVTGVHGISEHDYWIYKIDDFGNLLWEKAYGSYNEEAP
                     YDIIEVSTGGFLIAGYTWGDGGDVSGHHGITDYWILRIDNSGNIIWQNCFGSSGEDIA
                     YSVVETESSNFICAGITYGDDGDVTDFHGLNDTWVIKITYEGELIWQKCYGGSNFDET
                     HSIFDYSPNQLIFIASSSSTDGDITENIGDGDFWIVAI"
     gene            complement(1321..>2005)
                     /locus_tag="PLB46_16425"
     CDS             complement(1321..>2005)
                     /locus_tag="PLB46_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78166.1"
                     /translation="QRSTHAIAGIEFDPSDYIEVNIEPYIKYFNQLIEINRNKTTLRD
                     PDFATETGQAYGIDFSLKYERKAVYLWMAYSLGFIERNDGEQVYPPHYDRRHNLNLVA
                     TYSIKKKWEFSARWNLGSGFPFTQTAGFYESVDFLDGISTDYTTSNGSLGIIYDDELN
                     GGRLPYYHRLDLAVKRTFNFANKNKVEMNAGVTNAYNRENIFYFDRVRYERINQLPIL
                     PSLSVSYSF"
BASE COUNT          678 a          410 c          281 g          636 t
ORIGIN      
        1 aattgctaca atccagaaat caccgtcacc aatgttttca gttatgtcgc catctgttga
       61 tgatgaagat gcaataaaaa tcaattgatt tggagaataa tcaaaaatcg aatgagtttc
      121 gtcaaaatta cttcccccgt aacacttctg ccatatcaat tcaccttcat atgtaatttt
      181 tattacccaa gtatcgttta aaccatgaaa atctgttaca tcaccatcat cgccataagt
      241 tatcccagca caaataaaat tactactttc tgtttcaaca acactatacg caatatcctc
      301 accagaactg ccaaaacaat tttgccaaat tatattaccc gaattatcaa tcctcaatat
      361 ccaataatcg gttattccat gatgtccact aacatcgcct ccatcgcccc aagtgtagcc
      421 ggctataaga aaccctccgg ttgaaacttc aattatatcg taaggggctt cctcgttgta
      481 tgagccatat gccttttccc aaagtaaatt cccgaaatca tctattttat atatccaata
      541 atcatgttct gatatgccat gaacgcctgt aacgtcaccg tcttgtgaca tagattgggc
      601 agcgacaata tagccaccag ttgaaatttg tttaattgaa taaatgccat caggcgaact
      661 accaccgtat gttttttgcc aaataatttc accaaggata tcaattttaa ttacccaaca
      721 atccgtagag ccttgattaa gtgttacatc tccatcgttt gaaaaagttg agcctgctaa
      781 aataaacccg ccatcatttg ctagacaaac tgatgaacag acttcatagg aggatccacc
      841 ataacttttt tcccaaagta tttctccatc tgaattctgc attactgccc aaaaatcgat
      901 gagaccatgg ttgaaagata catctccatc aaaggagtct gaatatccaa ctgttaatat
      961 atttccctca gcatcctgca caatatcctg agctctgtca tcaaaatttc caccaaatgg
     1021 agattcccaa attatttcag gaacctgaca aatacatgga aatttaatac aacaaaaaaa
     1081 acttaccaag acgattatac aatatggaat ccgcatttat ttaacttatt ttgctaattt
     1141 aaccttattt ttcaaagctt aataaattta aaaatattca atacagttgt cctatatgac
     1201 aaaactttca aactcgacaa taaatataca ctaaacttgg taaagaatgt ttcattattc
     1261 attctaaaaa caaaaaaacc gcaattttta ttgcggtttt taataggagt atatgtttta
     1321 ttagaaagaa taggataccg acaaagatgg taaaattggt aactgattaa tacgttcata
     1381 acgcaccctg tcgaaataaa atatgttttc tctgttatag gcattggtaa cacctgcatt
     1441 catttctact ttatttttat tagcaaagtt aaacgtacgt tttacggcta agtccaaacg
     1501 atgataataa ggtaatctgc caccatttaa ttcatcatca taaataatac ctaatgagcc
     1561 gttgcttgtt gtgtaatcgg tgctgatacc atcaagaaaa tcaacacttt cataaaatcc
     1621 agctgtttgt gtaaatggaa atccggaacc taaattccat cttgcactaa attcccattt
     1681 ctttttaatg gaataagtag ccactaaatt caggttatgg cgacgatcat aatgtggcgg
     1741 atatacttgt tctccatcgt tgcgttcaat aaatcctaat gaataagcca tccataaata
     1801 aaccgcttta cgttcatatt ttaatgagaa gtcaatacca tatgcttgcc cggtttcagt
     1861 tgcaaaatca gggtcgcgta atgtggtttt atttctgttg atctcaatta attgattaaa
     1921 atatttaata tatggttcaa tattcacttc aatataatca gaagggtcaa attcaatacc
     1981 ggcaatggca tgtgttgaac gttgt
//
LOCUS       DAORKW010000640         1992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3645_length_1992_cov_4.006195, whole genome shotgun sequence.
ACCESSION   DAORKW010000640 DAORKW010000000
VERSION     DAORKW010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3645_length_1992_cov_4.006195"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLB46_16430"
     CDS             complement(<1..649)
                     /locus_tag="PLB46_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78167.1"
                     /translation="MTDRTAIDTIRGYFYQFDYSIISLLKLSNGSASILIEGVEDIDV
                     TTASETTAIQCKYYEKTEYNHSVIAEPIRLMLNHFKEVKLGNEPELKYKLRGHFKSGH
                     SKLVLPLSLQYLKDNFLTYTRTEKISNVNTKVKHFHHIELGLSDPELSEFIGLLEIDI
                     NAVEFEKQYKEIISLFKNTFNCSDISSELYFYNSALRVIRDVSKDQNKANRSISRD"
     gene            complement(1285..1629)
                     /locus_tag="PLB46_16435"
     CDS             complement(1285..1629)
                     /locus_tag="PLB46_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HRF78168.1"
                     /translation="MSNIPFQTIDWTTIEKVEYKGETGLAYWQTVQFEGLRIRLVEYS
                     SGYLADHWCQKGHIVHCLEGEFISEMSNGEKIKLNKGETYIVSDELSSHRSATAGGVK
                     FLIIDGDFLKLG"
     gene            complement(1761..>1992)
                     /locus_tag="PLB46_16440"
     CDS             complement(1761..>1992)
                     /locus_tag="PLB46_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78169.1"
                     /translation="RQKPYYMDKQLEAVNAATYLLVGDKDLLFPYQKSVDNAKRKIKS
                     LKATKIFTNVGHGIEIYDKALNYIGEIIKNYH"
BASE COUNT          612 a          342 c          304 g          734 t
ORIGIN      
        1 catctctcga aatgcttcta tttgctttgt tttggtcttt tgatacatct cttattactc
       61 ttaaagcact gttgtaaaaa tagagttcgg acgaaatatc gctacaatta aatgtattct
      121 taaaaagact tataatttct ttgtattgtt tttcaaattc aaccgcattt atgtcgattt
      181 caagtaatcc aataaattca cttagttcgg gatcactaag accaagctca atgtgatgaa
      241 aatgtttcac cttagtattt acattactga ttttttcagt acgagtgtag gttaaaaagt
      301 tatcctttag atattgaaga cttaaaggca aaactagttt tgaatgacct gatttaaaat
      361 gacctctcaa tttgtatttc aattcaggtt catttcccag tttaacttct ttaaaatgat
      421 ttagcattaa tctaataggc tctgcaatta cagaatgatt gtattctgtt ttctcgtaat
      481 atttacattg aattgcagtt gtttctgagg ctgttgttac atcaatatct tctacccctt
      541 ctattaaaat agatgctgaa ccatttgaca attttaatag tgagattatt gaataatcaa
      601 attgatagaa gtatcctcga attgtatcta ttgcagttct gtcagtcatt ttgttttttc
      661 gtgtttggta tcgtttttag catgaggcat aactttttat atacgtaact caaattacac
      721 aaatccctcc aattcagccg gatatacgca taaaatgagc atatttagta ttcaaggttg
      781 gttctataaa tgtagacaat ttgtgattat tgttaaaaac aaatgcctca tattcacggt
      841 tttccgttaa tccagaattg tcacaatttt gaataaactt gtatttaaaa tggctttgaa
      901 atggcaatta caggcatcaa agcaacaatt tcgctccttt tatattgtca caaacaaagt
      961 tcaaatcata cgcaactaaa atagtatagt ttcaaaaaca cgcttaattg tgtgtaatct
     1021 acaactccat tgggttgcaa ggatgaatgt atttaatact ttaatttcaa cccgtagatt
     1081 tactttttat ggtatccagt tattccttta taaacctata ataatggata ttgcgacaat
     1141 atctgattag aaataggtta ttaattcata tcaacaaccc gactttgtgt aaggcaggaa
     1201 aatatcttca acgcccattc agggcttatt ataaataacc aaaaataaaa gtcgccggca
     1261 tttgtacttt agatgtaatt catatcaacc caatttcaaa aaatcgccgt ctataattaa
     1321 aaattttacg ccaccggcag ttgctgaacg atgcgagctt agttcgtcag atacaatata
     1381 tgtttcgcct ttgttgagtt taattttttc accgttgctc atttcgctga taaattcacc
     1441 ttctaaacaa tgtacaatat ggcctttttg acaccagtgg tcggctaaat aaccggagga
     1501 atattctaca agtcgtattc ttaaaccttc aaattgaaca gtctgccaat aggctaatcc
     1561 tgtttcacct ttgtattcaa ctttttctat ggtggtccag tctattgttt gaaaaggtat
     1621 attgctcata ttgttgagtt atgttgaata ggtattgcta attaaacttg cgcattaaca
     1681 tttatgggtt gaggtaacgc atatttaagc aaacaaaaaa acaatatggc tctttaacgc
     1741 gcttttctca ataccgcttg ttaatggtaa ttttttatta tttccccaat ataatttaaa
     1801 gctttgtcgt atatttctat accgtgtccc acattggtaa atattttggt tgccttcagc
     1861 gatttaattt tccttttggc attatcaacc gatttttgat aagggaacag caggtcttta
     1921 tcacccacca gcaaataggt ggcagcattt actgcttcaa gttgcttgtc catataataa
     1981 ggtttttgcc gg
//
LOCUS       DAORKW010000641         1980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3678_length_1980_cov_5.050909, whole genome shotgun sequence.
ACCESSION   DAORKW010000641 DAORKW010000000
VERSION     DAORKW010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3678_length_1980_cov_5.050909"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /gene="trmB"
                     /locus_tag="PLB46_16445"
     CDS             complement(<1..393)
                     /gene="trmB"
                     /locus_tag="PLB46_16445"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRF78170.1"
                     /translation="MRKSKLQRFAELDTFTNVLQNFNFLQPVVVNHEKQIVDPKGKWH
                     STIFKNNNPIILELACGKGEYTCEMALADKKSNYIGVDVKGNRMHRGAKRALEEKIEN
                     AFFLRTDINIIATFFEPGEVSEIWITFPD"
     gene            558..>1980
                     /locus_tag="PLB46_16450"
     CDS             558..>1980
                     /locus_tag="PLB46_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRF78171.1"
                     /translation="MSSLFRKKTISSVMQNLDVADDINHSSGLHRTLSVRDLTFFGVA
                     AIIGAGAFSSIGSAAASGGPGVIVLFMICGIACGFTAMCYAEFASRIPVAGSAYTYAY
                     TSFGEIVAWIIGWALLMEYSIGNIYVAFAWSDYFENLLNLMHIHLPSWLTQNYRSAHA
                     EMGDAWNNAPVIGGLHIIFDFPALLINFLITWLVYVGVKESRNISNLMVIIKLSIIVL
                     VIVVGIFYLDIDNWTPINDKGVESFFPNGFKGVMAGVAAVFFAYIGFDAVSTMAEEAK
                     NPQRDLPRGMIYSLVICTIVYIIVSLVLTGMAHYKSLAVGDPLAEVFKMHDVGWMEAL
                     ISVSAVAAMTSVLLVFQMGQPRIWMSMSRDGLLPKKFQTIHPKFKTPSFATIVTGLVV
                     GIPILFTDESFVLDFTSIATLFAFVLVCGGILLIPRREKEAGKFHLPYVNAKFIYPII
                     IVGVALILLLNGATKNYFPDLFNI"
BASE COUNT          534 a          378 c          383 g          685 t
ORIGIN      
        1 gtctggaaaa gtaatccata tttcactcac ttcaccaggt tcgaaaaatg tagcgataat
       61 attgatatct gtccgcaaaa aaaatgcatt ctcaattttt tcttctaatg cccgttttgc
      121 accacgatgc atacgattgc cttttacatc cacgccgata taattacttt ttttatcagc
      181 cagcgccatt tcacaagtgt attcaccttt gccacatgct aattctaaaa taatgggatt
      241 gttattttta aaaattgtac tatgccattt cccttttggg tcaacaattt gtttttcatg
      301 attcacgaca acaggctgca gaaaattaaa attctgcaat acattcgtga aggtatctaa
      361 ttcagcaaag cgctgtaact tactttttct caccaggttt ttatttgcgt tgcaaaggta
      421 taaaatcaat aaccatggtt tcactacttt ctccttacca cattttgttt gaagttgtta
      481 attcttgacg gcacgcagcg cttaatttgt ataaatctta taaacttcct acatttcgac
      541 ctcaactaca accacttatg agcagtttat tcaggaaaaa gaccatctca agtgtaatgc
      601 aaaacctgga cgtggccgat gatattaacc acagctccgg cttacataga accctttcag
      661 tccgcgattt aacctttttt ggtgttgccg ctattattgg cgccggtgct tttagtagta
      721 ttggaagtgc tgccgcaagc ggtggcccgg gggtaatcgt attatttatg atttgtggta
      781 ttgcctgcgg ttttactgcc atgtgttatg ccgaatttgc ttcgagaatt ccggtagcgg
      841 gaagtgcata cacctatgcc tatacttctt ttggtgaaat agttgcctgg attataggtt
      901 gggctttgtt aatggaatat tccataggta atatatatgt agcatttgca tggagcgact
      961 acttcgaaaa cctgctcaac ctgatgcata tccatttacc ttcatggctt acacaaaatt
     1021 atcgctccgc gcatgccgaa atgggtgatg catggaataa tgctccggta attggcggct
     1081 tacatattat tttcgatttc cctgcattgc ttattaattt tttaattacc tggctggtgt
     1141 atgttggcgt aaaggaaagt cgcaacatca gtaaccttat ggtgataatt aagttatcta
     1201 tcatcgtttt agtaatagtt gtcggaattt tttatttaga tattgataac tggacaccaa
     1261 taaatgataa aggtgtagaa tcatttttcc cgaacggatt taaaggtgta atggccggag
     1321 ttgcagcagt gttttttgct tatatcggtt ttgatgcggt aagtaccatg gccgaagaag
     1381 caaaaaatcc tcaacgcgat ttaccccgtg gtatgattta ttcactggta atttgcacta
     1441 ttgtatatat aattgtttca ctcgtgctta ccggtatggc acattataaa tctttagcag
     1501 ttggtgaccc gcttgccgag gtatttaaaa tgcacgatgt gggttggatg gaagcactta
     1561 tttcggtaag tgcagttgcg gctatgacca gcgtactgct tgtgttccag atgggtcagc
     1621 cacgtatttg gatgagcatg agccgtgatg gattattacc taaaaaattc cagacaattc
     1681 atccgaaatt taaaacacct tcgtttgcaa ctatcgttac cggtttggta gtaggtattc
     1741 cgattttatt tacagatgaa tcgtttgtgc tcgactttac cagtattgca actttatttg
     1801 ctttcgtttt agtttgcggt ggtattttat taattcctcg tcgcgaaaaa gaagctggaa
     1861 aatttcattt gccttatgta aatgctaaat ttatttaccc aataataatt gttggtgtcg
     1921 cactaatcct attattaaat ggcgccacca aaaattattt tcccgattta tttaacatca
//
LOCUS       DAORKW010000642         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3708_length_1972_cov_3.807512, whole genome shotgun sequence.
ACCESSION   DAORKW010000642 DAORKW010000000
VERSION     DAORKW010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3708_length_1972_cov_3.807512"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1972
                     /locus_tag="PLB46_16455"
     CDS             <1..>1972
                     /locus_tag="PLB46_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78172.1"
                     /translation="PNILYAGSAGGGLWRSPELGGSWFPLTDNIPVTGIADVAVDPSN
                     NHVIYVATGDGYGYEASWQADNDFWGGVYSAGVFKSVDNGFTWLPTGLSYLQQELLII
                     QRLIIHPTNTNILLAATRDGIYRTTDGGDNWTMVEDKHCYDFAFKPTDNNTIYAVGEM
                     DVLISTDAGENWTILKDNLFGLNDRMSIETTADNPNVLYVFGAGASIRKSSDGGASWT
                     ASTVPFDVNTYYGYYDNVLGVSPLDEQLLYTGGLDLARSTNSGSTWVRKSSWWNWGTS
                     EYVHADFKGIICDPVNVNTLYVCNDGGVFKSTNKGESWVDISAGLRVGQIYRISPSFT
                     DPQQVMSGWQDNGCNRWDGTSWERVQGGDGMDVVIDHALESRIYASYQYGDLWRSTNN
                     GASWTDCGNTGGPWLTPVTMDPNNHLVMYAGRSDGQIDKSTNGGTSWNVIDPDLGGEV
                     FEIAVAPGNSDYVYACALQRIKVSTNGGSTWTNITAGLPTGGVGFNYIAVSDENPQHV
                     WVAISAYSDGDKVFFSNNAGSSWTNISGSLPNVPVNTIVYENVSETDRVYIGTDIGVF
                     TKDNLAADWEPYMTGLPNVMIHELAINYSDSKLYAATYGRSVWKSDLYNYAAPSLTVS
                     VPGTTFCLNQSVDIAYTAVGAFTAGNVFAIELS"
BASE COUNT          551 a          345 c          485 g          591 t
ORIGIN      
        1 tcctaatatt ttatatgcag gctctgccgg tggtggttta tggcgttcgc cggagttagg
       61 tggatcctgg tttccgctta ccgataatat ccctgttacg ggtattgccg atgtggctgt
      121 agacccttcg aacaatcatg taatctacgt tgcaacaggc gatggttatg gatacgaggc
      181 ttcttggcag gcagataatg atttttgggg aggtgtttac tcagctggtg ttttcaaatc
      241 agttgataac ggatttacct ggttaccaac cggtttgagt tatttgcaac aggagctgtt
      301 aattattcaa cgattgatta ttcatcctac caatacaaat attttattag ccgctacgcg
      361 agatggcatt tatcgaacaa ccgatggtgg cgataactgg actatggttg aggataaaca
      421 ttgttatgat tttgccttca aaccgactga caataatacg atttatgcag taggtgagat
      481 ggatgtttta ataagcaccg atgcaggtga aaactggacc atactgaagg acaatttatt
      541 tggtttaaat gacagaatgt ccattgaaac tacagctgat aatccaaacg tgctttatgt
      601 gtttggtgca ggtgcttcca ttcgcaaaag cagtgatggt ggagcctcat ggactgcaag
      661 cactgtacct tttgatgtta atacgtatta tggctactat gataatgtgc tgggtgtttc
      721 cccattggac gaacaattat tgtataccgg tggattagat ttggcgcgtt ctacaaatag
      781 cggttccact tgggttcgaa agtcttcatg gtggaattgg ggtacctctg aatatgtgca
      841 tgccgatttt aaaggaataa tctgcgaccc ggttaatgtg aatactttat atgtatgtaa
      901 cgatggtggt gtttttaaat caaccaataa aggtgagtca tgggtggata ttagcgcagg
      961 cttacgcgtt ggtcaaattt atcgtatttc accatcattt accgacccgc aacaagtaat
     1021 gagtggatgg caagataatg gttgtaaccg ctgggatggc acatcctggg agcgagttca
     1081 gggcggtgat ggtatggatg tagtgattga ccatgcgttg gaaagcagaa tttatgcttc
     1141 ttatcaatat ggtgaccttt ggagatcaac caataacggg gcttcctgga cggattgcgg
     1201 caataccgga ggtccatggc ttacaccggt aacaatggat ccaaacaacc atttggtaat
     1261 gtatgcaggt cgctcggacg ggcaaatcga taaatcaact aatggcggta cttcatggaa
     1321 tgtgattgac cctgatttag gaggtgaagt attcgaaata gcagtagctc ccggtaatag
     1381 tgattatgtt tatgcatgtg cattgcaacg aattaaagta tctactaatg ggggttctac
     1441 ctggactaac ataacggcag gattaccaac cggaggagtt ggatttaatt acatcgcagt
     1501 tagtgatgaa aatccacaac atgtttgggt agcaatcagt gcctacagcg atggagataa
     1561 agtgtttttc tctaataatg ctggtagcag ctggacaaat atttcaggct cattaccgaa
     1621 tgttccggta aatactattg tatacgaaaa tgtaagtgag acagaccgcg tttatattgg
     1681 aactgatatt ggcgtattta cgaaggataa tctagcagct gattgggaac cttatatgac
     1741 aggtttgcca aatgtgatga tacacgaact ggcaattaat tatagcgata gtaaattata
     1801 tgcagccact tacggaagaa gcgtttggaa atcggattta tacaattatg ccgcaccttc
     1861 attaacggtt agcgttccgg gaacaacatt ctgtttaaat caatcggttg acattgcata
     1921 cactgcagtt ggtgcattta ctgccggaaa tgttttcgca attgaattga gc
//
LOCUS       DAORKW010000643         1971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3709_length_1971_cov_5.661273, whole genome shotgun sequence.
ACCESSION   DAORKW010000643 DAORKW010000000
VERSION     DAORKW010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3709_length_1971_cov_5.661273"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1068)
                     /gene="rfaD"
                     /locus_tag="PLB46_16460"
     CDS             complement(70..1068)
                     /gene="rfaD"
                     /locus_tag="PLB46_16460"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HRF78173.1"
                     /translation="MATDLHTDRPLILVTGALGFIGSCLALELLRSGKYRVVICDDLN
                     ATQKFPNIADAEFEMRIDRKALIALLQAGGLPDLKGVLHIGARTDTTEFNKTIFDELN
                     YGYSQAIWQICSERKIPLVYASSAATYGDGAFGYSDDHAVIEKLVPLNPYGDSKNDFD
                     KWVLTQKDTPPHWYGLKFFNVYGPNEYHKSRMASVIFHAYHQFKNTGKVKLFRSHKAA
                     YKDGEQIRDFIYVKDVIRVIEWLMEHHPESGIYNLGTGKARSFYDLAAATAKAMNMPL
                     VIDWIDIPEDIREKYQYFTEADMHKLISAGYNNTFYNLEEGIDDYVKSYLIPAIHY"
     gene            1104..1580
                     /locus_tag="PLB46_16465"
     CDS             1104..1580
                     /locus_tag="PLB46_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78174.1"
                     /translation="MRYGLVLMLASLVFISCNKKKYERVTSYNDKVVSYIEQSERTMK
                     VWNTTNFMQEYAIKKQNSVTRLLNMQDSLNNLTPLEDDDTLRLAGLSMIENYLNAFAI
                     YDTVHKMLSDSLFLKEDSIRVQELLRSNQNMLKQQAESFMETQKRFSARYELPFLE"
     gene            complement(1586..>1971)
                     /locus_tag="PLB46_16470"
     CDS             complement(1586..>1971)
                     /locus_tag="PLB46_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78175.1"
                     /translation="DELWNLIRINPDKLPKGEMQILPGAMYLRLSHQPLEPVKANLDL
                     RDENGIMVYVIDFPTLERQLNIRFEKTFPYKITGWDDTYAGFDGKKLTTTAVRMKDMM
                     LDYWAAHNNDDRVLREQLSLPKDTQ"
BASE COUNT          633 a          395 c          306 g          637 t
ORIGIN      
        1 gaagtatacc aactagaaaa tacctcagga actttgttac ctagtatcgg tttaataatc
       61 gaactgtaat taatagtgta ttgcaggaat taaataagat tttacataat catcaatacc
      121 ttcttctaaa ttgtagaagg tattgttata tcctgcagat attaatttat gcatatctgc
      181 ttcagtgaaa tactgatact tttctctgat atcttcagga atatctatcc aatctattac
      241 taaaggcata ttcatcgctt ttgccgtagc cgctgcaagg tcataaaatg aacgcgcctt
      301 tcccgtgcct aaattataaa tgcccgattc cggatggtgt tccatcaacc attcaattac
      361 acgaattaca tcttttacat aaataaaatc gcgtatttgt tctccgtctt tatatgcagc
      421 tttatgtgag cggaataatt ttacttttcc ggtattttta aattgatgat aagcatgaaa
      481 tattacagat gccattcgcg atttatgata ctcgtttgga ccatacacat taaaaaattt
      541 taatccatac caatgtggag gcgtatcttt ttgtgttaat acccatttgt cgaaatcatt
      601 tttcgaatcg ccatatggat tcaaaggaac caatttttct atcactgcat ggtcatcgct
      661 atatccaaat gcgccatcac cataggtagc agcactggat gcatatacca atggaatttt
      721 gcgttcagaa caaatttgcc agatagcttg cgaataacca taattcaatt catcaaaaat
      781 agttttatta aactcagtag tgtcagttcg tgcaccaata tgtaaaacac ctttcaaatc
      841 aggtagccct cctgcctgca acaaagcaat tagtgccttt ctgtcaatac gcatctcaaa
      901 ctccgcatca gcaatattgg ggaatttttg tgttgcattc aggtcatcac aaataacaac
      961 ccgatacttt ccactgcgca ataactccaa ggccaaacaa ctgcctataa atcccaaagc
     1021 acctgtaacc agtatcaaag gcctgtcagt atgtaaatca gttgccattt gtgtaaattt
     1081 gcacaaatct aaagcaattt tttatgagat acggactcgt tttaatgctt gcttcgctcg
     1141 tttttatttc ttgcaataaa aagaaatatg aacgtgtaac gtcttataac gacaaagtgg
     1201 tgagttatat cgaacaaagt gaacgcacca tgaaagtttg gaatactacc aactttatgc
     1261 aggaatatgc tattaaaaaa caaaattccg taacacgttt actcaacatg caggattcat
     1321 taaataacct gacaccattg gaagatgatg atactttgcg tttggcaggt ttatctatga
     1381 tagaaaatta cctcaatgct tttgccattt acgataccgt acacaaaatg ttatcggata
     1441 gtttatttct aaaagaagat tcaattcggg tacaagaatt attacgttct aaccaaaaca
     1501 tgctaaaaca acaagctgaa tcttttatgg aaacacaaaa aagattttct gctcgttacg
     1561 aattaccatt tttagaataa aacaattatt gtgtatcctt tggtaaactg agttgctcgc
     1621 gtaatactct atcatcatta ttatgagctg cccaatagtc caacatcata tctttcatac
     1681 gcaccgcggt agttgttaat ttttttccat caaaaccggc ataggtatcg tcccatcctg
     1741 tgattttata tgggaaagtt ttttcaaacc ggatatttaa ctgacgctct aatgttggaa
     1801 aatcgattac ataaaccatt atcccatttt catcgcgcaa atctaaattt gccttaacgg
     1861 gttccaatgg ttggtgactt aaacgcaaat acatggcgcc cggtaaaatt tgcatctccc
     1921 ccttcggcaa tttatcagga tttattctaa taagattcca caattcatcc t
//
LOCUS       DAORKW010000644         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3723_length_1969_cov_4.695925, whole genome shotgun sequence.
ACCESSION   DAORKW010000644 DAORKW010000000
VERSION     DAORKW010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3723_length_1969_cov_4.695925"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PLB46_16475"
     CDS             complement(<1..984)
                     /locus_tag="PLB46_16475"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78176.1"
                     /translation="MQIGLMLIVSSITFTSCVKTGCEREYNYVAYRPMYMSYEDLRSA
                     VAVEGPRKMESTGKIYYYAPYLFVNEINEGIHIVDISNVSAPVITGFINIPGNVDIAM
                     SGTTLYADSYIDLVALDVSNMNAITIVDREQNVFPYRVDENIHVDVDETKGVVDGWVG
                     TDTVITMECGSMDSYFFPTDVVFLSESSAAFEGAPGVNGSKGGSMARFTVDNNYLYCL
                     SENTMELFNVTNQNNPVHSGDVPMPLNIETIFPHEDYLFIGSTTGVSIYNNSSPFNPT
                     FVSIFEHATSCDPVVVKGNYAYSTLRSGNQCAGFSNQLDIIDISNISYPTLV"
     gene            complement(1166..1591)
                     /locus_tag="PLB46_16480"
     CDS             complement(1166..1591)
                     /locus_tag="PLB46_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78177.1"
                     /translation="MEGAMLLQKSAIPASTMLLDNCTHAIEQGNFEEALNICIQFLEN
                     FPVNNSNRDKNSIGYHNYLRLCSIMMNDNHHYAAVINLLNIKQSVIDFAMSFFIEIGK
                     KGIHFPKPVVQKEDVLFVFVFCKQIQQLILEKLQRHPEN"
     gene            complement(1676..>1969)
                     /locus_tag="PLB46_16485"
     CDS             complement(1676..>1969)
                     /locus_tag="PLB46_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF78178.1"
                     /translation="DNVKQRFGKFQIPGSWMHTDVQEIVSLFNQHPNVKLCISGHIHL
                     QDKVQYNNVTYCCNGAVSGDWWRNEYYHQTKAGYAIIDLFSDGTFENTYMHYA"
BASE COUNT          681 a          369 c          292 g          627 t
ORIGIN      
        1 aacaagtgta ggatatgaaa tgttagaaat atcaataata tctaattgat tagaaaaacc
       61 tgcacattga ttgccacttc tcagggtgct ataggcataa ttgcctttca ctaccacagg
      121 atcgcaactt gtagcatgtt caaaaatgga aacaaaagtt ggattaaatg gtgaactatt
      181 attataaata gaaaccccag ttgtcgaacc aatgaacaaa taatcttcat gcgggaaaat
      241 tgtttcaata ttcaaaggca ttggaacatc acctgaatga acagggttgt tttgattggt
      301 tacattaaat aattccattg tattttctga taaacaatac aaataattat tatctacagt
      361 aaatctggcc atggaaccac ctttactgcc attaacgccc ggagcgcctt caaaggcggc
      421 tgaagattct gacaaaaaca ctacatcggt tgggaaaaaa taagaatcca tactaccaca
      481 ttccatagtg ataaccgtat cagtaccaac ccatccatcc acaacacctt ttgtttcatc
      541 tacatctaca tgtatatttt catcaactcg atatggaaaa acattttgtt cgcggtctac
      601 aattgtaatg gcattcatat tacttacatc caatgcaact aaatcgatat aactatctgc
      661 atataacgtt gttccactca tagcaatatc tacatttccc ggaatattaa taaatccggt
      721 aattaccgga gcagacacat tgctgatatc aacaatatga ataccttcat ttatttcgtt
      781 cacaaataaa taaggtgcat agtaataaat tttaccggtc gattccattt tccgaggacc
      841 ttccacggca actgcagagc gcaaatcttc ataactcata tacatcggcc tatatgcaac
      901 ataattgtat tcgcgttcac aaccggtttt tacgcaggaa gtaaatgtaa ttgaggagac
      961 aatcagcatt aagccgattt gcaaaaggga cttttggtgc aatttcatat gtttgctttt
     1021 gttgtatcaa aattaataaa atctaacaaa aagctataat accaagccta tttgagcagg
     1081 ttttacaatt tattgacatt cagcactata aatatcaatt tttttataaa cttaaaataa
     1141 tggaatcgta gtttattcaa tcgaactaat tttccggatg tcgctgcaat ttttctaaga
     1201 tcaattgctg aatttgtttg caaaaaacaa agacaaaaag cacatcttct ttttgcacca
     1261 ctggctttgg aaagtgaata ccttttttgc caatttctat aaaaaaagac atcgcaaaat
     1321 caataacaga ttgtttaata ttcaataaat tgataactgc agcataatga tggttatcgt
     1381 tcatcataat agagcataga cgcaaataat tatgataacc aatactattt ttatcgcggt
     1441 tggaattatt aaccgggaag ttttctaaga actgaataca aatatttaat gcttcttcga
     1501 aattgccttg ttcaatagca tgagtacaat tatccaataa catagtagat gcaggaatag
     1561 ctgatttttg taataacata gcaccttcca tacctattaa tttatgtgta aaataacaaa
     1621 ccgtttaccg gataatactt atctccgtat taattaatta ttacccctgt gtaaattagg
     1681 cgtaatgcat ataggtattt tcaaatgtgc catcgctaaa caaatcaata atcgcataac
     1741 ctgcttttgt ttgatgataa tattcatttc tccaccaatc gccggaaaca gcaccgttac
     1801 aacaatatgt tacattattg tattgcacct tatcttgtaa gtgtatatgt ccgctgatac
     1861 acagttttac gttaggatgc tgattaaata acgaaacaat ttcctgcaca tcggtatgca
     1921 tccacgaacc cggaatttga aattttccaa accgttgttt tacattatc
//
LOCUS       DAORKW010000645         1967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3731_length_1967_cov_3.939331, whole genome shotgun sequence.
ACCESSION   DAORKW010000645 DAORKW010000000
VERSION     DAORKW010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3731_length_1967_cov_3.939331"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /gene="smpB"
                     /locus_tag="PLB46_16490"
     CDS             complement(<1..331)
                     /gene="smpB"
                     /locus_tag="PLB46_16490"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRF78179.1"
                     /translation="MKPAELNIRNKRATFEYEIIDRYVAGIVLRGSEIKSVRDGRASI
                     NEAYCMFKEGELWVKNLNISEYKQATVWQHEPLRMRKLLLSKKELGKLLAKVKERGYT
                     IVPLKMFL"
     gene            complement(374..769)
                     /locus_tag="PLB46_16495"
     CDS             complement(374..769)
                     /locus_tag="PLB46_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78180.1"
                     /translation="MVNPFKTGDSKVFTHIVEMADIAAFESGVVHQVYSTFCLTRDAE
                     WSTRLFVLEMKEADEEGIGTFIEVMHRSPAKVGSTVNFYATLEAVNGHEIICSFIAKC
                     GERIIAEGKTGQKILKKEKLNRIMAAIEN"
     gene            856..1785
                     /locus_tag="PLB46_16500"
     CDS             856..1785
                     /locus_tag="PLB46_16500"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRF78181.1"
                     /translation="MKGMRLYFFIAVLANLILVSCKQETSSGNDKLQIVATTGMIGDA
                     VAIIAGDKAEVTVLMGPGVDPHLYKATQGDLNALRSGDIIFYNGLHLEGKMQEVFDRL
                     ATTKKVFPVAAGIPESKLRTVAQVNGISTHDPHIWFDVQLWMMAVAEIGKQLSASDPT
                     NATFYATQTTKYLEQLKALDVFVKEKMSSIPETHRTLITSHDAFGYFGAAYGIRVKGL
                     QGISTAAEFGLKDITDMVNMIIAEDIKAVFVESSVSEKSIQAVIEGCHQKNHNIKAGG
                     TLYSDAMGAAGTPEGNYIGMVTHNVEVIYAALK"
BASE COUNT          583 a          376 c          391 g          617 t
ORIGIN      
        1 ttaaaaacat tttcagcgga actatagtgt agccgcgctc tttcaccttg gccagcagtt
       61 tgccaagttc ttttttactg agcaataatt tgcgcatgcg caatggttcg tgttgccaaa
      121 ccgttgcctg tttgtattca gaaatgttta aatttttcac ccataattcg ccttctttaa
      181 acatacagta tgcttcgtta atactggcac gtccatcgcg aacactttta atttcgctgc
      241 ctcgcaaaac aatacctgcc acataacgat cgataatttc gtattcgaag gtggcacgtt
      301 tatttctgat atttaattcg gcaggtttca tattatttta ttcaacatca ctattggcat
      361 gcaagttcgt tatttaattt tcaattgcag ccataatcct gtttagcttt tcttttttta
      421 atattttttg tcccgtttta ccttcagcaa tgattctttc tccgcatttg gcaataaatg
      481 aacaaattat ttcgtgacca ttaaccgctt caagggttgc ataaaaatta acggtgcttc
      541 ccactttagc aggtgagcga tgcattactt ctataaaagt gcctatcccc tcttcgtccg
      601 cttctttcat ttccagcaca aataatctgg tgctccactc cgcatcacgt gtaaggcaaa
      661 aggtagaata tacctgatgc actaccccac tttcaaatgc agctatatcg gccatttcaa
      721 cgatatgggt aaatacttta ctgtctcctg ttttaaacgg attcaccatg ttggggcgaa
      781 gttaaggatt tcataatttc aaacatccta aaaaatattt tagactaatc taaacttttt
      841 tattactttt ggggtatgaa aggtatgaga ctgtattttt ttatcgccgt tttagccaat
      901 ttgatattgg tttcctgcaa acaggagacc tcttcaggta acgacaaact tcaaattgta
      961 gccaccaccg gtatgattgg tgatgctgtt gccataattg ccggcgacaa agccgaggtt
     1021 accgtattaa tgggcccggg cgttgaccct cacttgtata aagccactca gggcgatttg
     1081 aatgctttgc gcagtggaga tattattttt tataacggtt tacatttaga aggcaaaatg
     1141 caggaagtgt ttgaccgttt ggcaacaacc aaaaaagtat ttccggtggc agcaggtatt
     1201 cccgaatcga aattgagaac agttgctcag gtaaatggga ttagcacaca tgaccctcat
     1261 atttggtttg atgtgcaatt gtggatgatg gcagtggcgg aaattggcaa acaactttca
     1321 gcatcagatc cgaccaatgc aacattttat gcgacacaaa caacaaaata ccttgagcag
     1381 ttaaaagctt tggatgtttt tgtaaaagaa aaaatgtcgt ccatacccga aacacatcgc
     1441 actttaatta catcacatga tgcatttgga tattttggtg cagcatatgg tattcgcgta
     1501 aaaggtttac aaggtatttc taccgctgcg gaatttggat tgaaggatat taccgatatg
     1561 gtgaatatga ttattgctga agatattaaa gctgtttttg tagaaagcag tgtaagtgaa
     1621 aaatccattc aggcagtcat agaaggttgt caccaaaaaa atcataacat taaagcaggt
     1681 ggtacattat acagtgatgc aatgggtgct gccggaacac cggaaggaaa ttatatcgga
     1741 atggtgacac ataatgtaga agtaatttat gcagctttaa aataatttga attaataacc
     1801 agcagaaatg atgcaaatac cggcgattga agtacacgat gtaacggttt cttacaatag
     1861 gaaacctgct ctttggggaa ttgattttgc agttccggaa ggcaaattgg taggtattat
     1921 cggaccaaat ggtgccggta aatcaaccat gataaaagcc atgatgg
//
LOCUS       DAORKW010000646         1964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3740_length_1964_cov_3.857517, whole genome shotgun sequence.
ACCESSION   DAORKW010000646 DAORKW010000000
VERSION     DAORKW010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3740_length_1964_cov_3.857517"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /gene="glgP"
                     /locus_tag="PLB46_16505"
     CDS             complement(<1..1002)
                     /gene="glgP"
                     /locus_tag="PLB46_16505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRF78182.1"
                     /translation="MIHYSNWHLPYEVNPAYQQRTAYFCMEFGVHQALKIYSGGLGYL
                     AGSHMRSAYELKQNLFGIGILWRYGYYDQVRDDHNYMAVQFQKKLYTFLQQTDIQFTM
                     QIDGKDVIVKVFYLAPEVFGSAPLFLLSTDFPENEEYAREYTHRLYDRNHEIRIAQNM
                     ILGIGGAKVVELLGGVDNYHMNEGHALAMTFYLFSKLKDVDEVRKRVVFTTHTPEKAG
                     NEEHDINEMNRMGFFSGIPLELVRHITKTEGDMLGYTPSALYMSKLSNGVSKLHGEVS
                     KEMWKDYKGICNITSITNAQQKKFWADKELNQAYINRDDAALVTIKKELKRELFEIVA
                     "
     gene            complement(1398..>1964)
                     /locus_tag="PLB46_16510"
     CDS             complement(1398..>1964)
                     /locus_tag="PLB46_16510"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HRF78183.1"
                     /translation="PSGIEVCEKQLLNMIHRVHEVEVNEAEIAKYLPVIEKEFADMTK
                     EDVLKKFASLEFNRFLEYYRFAEDLNIGERREVREYATKKGNNDLMFINLGKMDNLGA
                     KELVEMVKQVGNVRPHDIGDIRLKGAYSFFELPANQSQHVVQAFNGFVYRGRRVRVEI
                     QNERQEFPKKKAHKGGSREMKPYKGKRK"
BASE COUNT          573 a          412 c          360 g          619 t
ORIGIN      
        1 agcgacaatt tcaaataact cgcgtttgag ctcttttttg atggtaacca aagccgcatc
       61 atcgcggttg atgtaggctt gatttaactc tttatccgcc caaaacttct tttgctgcgc
      121 attggtaatg ctggtgatat tgcaaatgcc tttgtagtcc ttccacatct ccttgcttac
      181 ttcaccatgg agtttactca ccccattgga caacttactc atatataatg ctgaaggcgt
      241 gtagccaagc atatctcctt ctgttttggt aatatggcgg acaagttcca ggggaattcc
      301 tgaaaagaaa cccatacggt tcatttcatt gatatcgtgt tcttcattgc ctgctttttc
      361 gggggtatgc gtggtaaata caactcgttt gcgcacttca tccacgtctt taagcttgct
      421 aaacagatag aaagtcatag ccaaagcatg tccttcattc atgtgataat tgtccacacc
      481 acccaaaagc tccacaacct tagcgccgcc gatgccgaga atcatgtttt gagcaatacg
      541 tatttcatga tttctatcat ataatcggtg ggtatactca cgggcatact cttcattttc
      601 aggaaaatcg gtgcttaaaa gaaacaaagg tgcgctgcca aacacttcag gtgccaggta
      661 aaacaccttt acaatcacat ctttaccatc aatctgcatg gtaaactgaa tatcagtctg
      721 ttggaggaag gtatacagtt tcttctgaaa ttgaacagcc atatagttat ggtcgtctct
      781 cacctggtcg taatatccat atcgccaaag gataccaata ccgaacaggt tttgtttgag
      841 ttcatatgcc gaacgcatgt gcgaacctgc taaatatcca agtccgccgc tgtatatttt
      901 tagtgcctgg tgaacgccaa attccataca aaagtaggct gtacgttgct ggtaggcagg
      961 attaacttca taaggcaaat gccagttgct gtaatgaatc atcgtcttct ttaagtagtt
     1021 aagatgcgaa tatattgaat ataaacaaac agcggttggt ttggtaaggt ttgactgtgg
     1081 aaaaaggttg tgttttatac ttacttcacc tgtgaccaac gatttgaagt tgaaagcaat
     1141 ttatagtggt gtataaaaat caaaaaatat ctcatttaat acataaataa ttataattca
     1201 ataatttatg tataattata taaactaact aagtagataa attaaacaat atccccaaaa
     1261 ttaatgcatc taaattttta taaattgatg aaaatgaagt cacaaaaccc aaaaaaatgc
     1321 ctgttgataa tctttttgtc caaaatacaa aaaggcagtc tttgggggac tgcctctaaa
     1381 tttagtaata aattgaatta tttacgtttg cctttgtaag gcttcatttc gcggcttccg
     1441 cctttgtggg ctttcttttt agggaattcc tgacgttcgt tttggatttc aacacgaaca
     1501 cgacggccac ggtaaacaaa accattgaaa gcttgcacta cgtgctgact ttgattagca
     1561 gggagttcga agaaggaata ggcacctttt aagcggatat caccaatatc atgcggacga
     1621 acattaccaa cttgtttaac catttccacc aattctttag caccaagatt atccatttta
     1681 ccaaggttga tgaacatcaa gtcgttattg ccttttttgg ttgcatattc tctcacttca
     1741 cggcgttcgc caatattgag gtcctctgca aaacggtagt attctaaaaa ccggttaaac
     1801 tcgagtgatg cgaacttttt cagcacatcc tctttggtca tatcggcaaa ttccttttca
     1861 attaccggca ggtatttagc tatttctgcc tcgtttacct caacttcatg cacacggtga
     1921 atcatgttca acagctgttt ctcacacact tctatcccgc tagg
//
LOCUS       DAORKW010000647         1963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3742_length_1963_cov_3.672956, whole genome shotgun sequence.
ACCESSION   DAORKW010000647 DAORKW010000000
VERSION     DAORKW010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3742_length_1963_cov_3.672956"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLB46_16515"
     CDS             complement(<1..398)
                     /locus_tag="PLB46_16515"
                     /inference="COORDINATES: protein motif:HMM:NF027592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HRF78184.1"
                     /translation="MKKLVYSLLLVSGTIMMISCGGKDKKDDNADDMEDLTMNEGSVF
                     QFASYTPADSARTYGKAITADGANNIYNVAANKEKANGYTGKVTGKVTSVCQAKGCWM
                     TLDAGNGETMMVTFKDYGFFVPKDITGETVV"
     gene            complement(497..634)
                     /locus_tag="PLB46_16520"
     CDS             complement(497..634)
                     /locus_tag="PLB46_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78185.1"
                     /translation="MKKLFYLVVMAATFSLATSCKDKEKDHSEDEMDDLTLVVSVETF
                     A"
     gene            complement(641..1903)
                     /locus_tag="PLB46_16525"
     CDS             complement(641..1903)
                     /locus_tag="PLB46_16525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRF78186.1"
                     /translation="MAQILNTDVLILGAGPGGAATALFLAKENIPCIIVDKAVFPRDK
                     ICGDALSGKVVEILNRYDRSFVEKLSLDPIQLNCWGVTFVAPNVEELSIPFRNKPKKT
                     DEKREIAPGFITKRYDFDHFMVKEVKKHASVNLLEGVEIDQFEKTPDGFTCRDKSGNY
                     IIHAKLVIDGSGAHSQFAKKIGKLEVEKEHHCAGLRVYYKGVTGIKEGNYIELHFLKD
                     FLPGYFWIFPLADGQCNVGVGMRSDYVGKKRINLKTELNNIIQKYPQLSERFKNAEPG
                     DDIRGYGVPLGSKKRAISGDNYMLIGDAASLIDPFTGEGIGNAILSGYTAAQQAKEAI
                     ETKNFSGTALKKYDEAIYRRLGDELSLSYRMQKLVKYPWLFNFVVRKANRNKVLRETI
                     MVMFEDIDIRDRLRKPSFYFKLLFSGKN"
BASE COUNT          509 a          442 c          357 g          655 t
ORIGIN      
        1 actacagttt cacctgtaat gtctttaggc acaaaaaagc cgtaatcttt aaaagtaacc
       61 atcatggttt cgccattgcc tgcatctaat gtcatccaac atcctttggc ctggcaaacg
      121 ctggttactt taccggtaac tttacctgta taaccatttg ctttttcttt attagcagca
      181 acattgtaga tattgttagc accatctgcc gttatcgcct taccataagt tctagcgctg
      241 tctgcaggcg tatagctggc aaactggaac actgaacctt cgttcatggt taaatcttcc
      301 atatcgtcgg cgttatcgtc ttttttgtct ttaccaccgc atgatatcat catgatggtt
      361 cctgatacta acaggaggct ataaactagt tttttcattt ttaaaatttt atacaaagtt
      421 acactgattt tgagaaagaa ttgttctaaa tttttgtaaa aaggtgtgtg ctctcacgga
      481 gcaaatgttg tgccgattaa gcgaatgttt caacactcac cactagggta aggtcatcca
      541 tctcatcttc actgtggtct ttttccttgt ctttgcaaga ggtagccaga ctgaacgttg
      601 cagccattac caccaggtag aatagttttt tcatatgctg tcaattttta cccgaaaata
      661 acaatttgaa ataaaatgat ggctttctta acctgtcgcg gatgtcgata tcttcaaaca
      721 tcaccatgat ggtttcgcgc aaaaccttgt tgcggttggc ttttcggaca acaaagttga
      781 ataaccacgg atatttcact aatttttgca tacggtagct caggctcaat tcatcgccca
      841 ggcgcctgta aatggcctca tcgtattttt ttagggctgt accggagaaa tttttagtct
      901 caatggcctc ttttgcctgc tgagctgctg tataaccgct taggatggca tttccgatac
      961 cttcaccggt gaaagggtca atgaggctgg cagcgtcgcc aatcaacata tagttatctc
     1021 cgctaatagc tctttttttg cttcccagtg gtactccgta acctctgata tcgtccccgg
     1081 gctcggcgtt tttgaaccgc tcgctcagct gagggtattt ttgaataatg ttgttgagct
     1141 ccgtttttag attgatacgt tttttcccca cataatcgct cctcatgcct acacccacat
     1201 tgcactgccc gtcggccaat gggaatatcc agaaatagcc cggtaaaaag tcttttaaaa
     1261 agtgaagttc gatatagttg ccttctttga tgcctgttac gcctttgtaa tacacgcgta
     1321 acccggcaca atgatgttct ttctcaacct caagtttccc tattttttta gcaaactgag
     1381 aatgtgctcc actgccgtct atgactaatt tggcatgtat aatataattg cccgatttat
     1441 ctctgcaggt aaaaccgtca ggcgtttttt caaattgatc aatttcaact ccttccaaca
     1501 aattaacact tgcatgtttt tttacttctt ttaccataaa atggtcgaag tcgtaacgtt
     1561 tagtaataaa ccccggtgct atttcccttt tttcatcggt ttttttgggt ttattgcgga
     1621 aaggaatact taattcttct acattgggtg ccacaaatgt tacgccccaa caattcaatt
     1681 ggatggggtc cagactgagt ttttcgacaa aactgcgatc gtaacgattc aatatttcta
     1741 ctacttttcc gcttaatgca tcaccacata ttttatctcg tggaaatact gctttatcca
     1801 ctataataca gggaatattt tctttcgcta aaaataatgc tgttgcggcg ccaccgggac
     1861 ctgcacctaa tattaaaaca tcggtattga gtatttgtgc cattgggcgc aaagatagac
     1921 agaacctatc tcaaaactac aatagcgcta aattttcctt cat
//
LOCUS       DAORKW010000648         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3755_length_1959_cov_5.375000, whole genome shotgun sequence.
ACCESSION   DAORKW010000648 DAORKW010000000
VERSION     DAORKW010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3755_length_1959_cov_5.375000"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLB46_16530"
     CDS             complement(<1..512)
                     /locus_tag="PLB46_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HRF78187.1"
                     /translation="MQTEKGKHIDKITAASLLVALGIIYGDIGTSPLYVLKAVVLDRN
                     IEEVLVLGGVSCIFWTLVFQTTLKYIVLTLRADNEGEGGIFSLYSLVRRYGKKLVIPT
                     ILGATTLLADGIITPPISVSSAVEGLEIVPGLEHIPTIPIVIGILSLIFFMQRFGTQK
                     VGSAFGPVMV"
     gene            complement(523..1206)
                     /locus_tag="PLB46_16535"
     CDS             complement(523..1206)
                     /locus_tag="PLB46_16535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRF78188.1"
                     /translation="MNNPEVLIIDDELPICKLLVITLESSDYKTIVANSAKEGLIKAA
                     NHPPDIILLDLGLPDASGHDVLKQLREWYNKPIIILSVQSEESDIVLALDEGANDYLI
                     KPFRTGELLARMRSALRNMYAENENNVFISNHMEVDLVARTVRIHGELVKLTATEYTL
                     LALFVKNEGKVLTHQYLLREVWGPGYIQQSQYLRVFIAQLRKKIETDPNNPTIIITES
                     GVGYRLSGE"
     gene            complement(1199..>1959)
                     /locus_tag="PLB46_16540"
     CDS             complement(1199..>1959)
                     /locus_tag="PLB46_16540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF78189.1"
                     /translation="NKIRTTERQTQQKEGEENTLKLYNTLLNSLSHELKTPIATIIGA
                     TDNLQTENIKLTETNRKELTTEIAQAAWRLNRQVENLLNMSRLESGVIAPKRDWCDIN
                     EIAYEVVNRLKDDYATHPFIVSIKDDVPLFKIDHGLIEQILYNLAVNAAVYTPKHAVI
                     TIKAFSQEEHLVLIVQDNGNGFPEEAIEKVFDKFYRVKYSAAGGTGLGLSIVKGFVEA
                     QGGSVTLVNMPEGGAKFTIVIPAETTYLKNLKNE"
BASE COUNT          584 a          392 c          316 g          667 t
ORIGIN      
        1 attaccatca ccgggccaaa ggcactgcct actttttggg taccaaatcg ctgcataaaa
       61 aatatgagcg ataaaatacc aattacaatt ggaatagtag gaatatgttc taaccccgga
      121 acaatttcca aaccttccac tgcagatgaa acagaaatag gtggagtaat aatgccatca
      181 gccaataacg ttgttgcgcc taaaatagta ggaatgacta attttttacc atatctcctt
      241 actaatgaat acaaggagaa aataccacct tccccttcgt tatctgcgcg tagtgtaagt
      301 acaatatatt ttaaagtagt ttgaaaaacc agcgtccaaa atatacacga tacaccacca
      361 agtactaaaa cctcttcaat atttctatct aataccacag ctttgagcac atataaaggg
      421 ctggttccaa tatcgccata aataatgccc aatgctacca acaaagatgc cgctgtaatt
      481 ttatcaatat gttttccttt ttctgtttgc atagctatta ttttattctc ccgataatcg
      541 atatcctacc ccggattctg tgattattat ggttggatta tttggatctg tttcaatttt
      601 ttttctgagt tgtgctataa atacacgtaa atattgtgac tgttgaatat aacccggtcc
      661 ccacacctca cgcaacaaat attgatgagt caacaccttg ccttcgtttt ttacaaaaag
      721 tgctaagagt gtatattcag ttgcagttaa ttttaccagt tcaccatgaa tgcgcacagt
      781 tctcgctacc aaatctactt ccatgtgatt ggagataaat acgttatttt cattttcagc
      841 atacatatta cgcaatgcag aacgcattct tgcaagtagt tctcctgtcc gaaaaggttt
      901 aattaaataa tcgtttgcgc cttcatccaa tgccaatact atatcggatt cttctgattg
      961 taccgataaa ataataatgg gtttattata ccattcacgt aactgtttta atacatcatg
     1021 tccgctggca tcgggcaaac cgaggtctaa taaaataata tccggaggat gattagctgc
     1081 ctttatcaat ccctctttag cagaattagc tacgatagtt ttataatcgc tgctttccag
     1141 tgtgattacc aataatttac aaattggtaa ttcatcatct ataattaaga cctccggatt
     1201 attcattttt caaatttttt agataagtag tttctgcagg aattacaatg gtaaatttcg
     1261 ctccaccttc gggcatattc actaaggtta ctgaacctcc ttgggcttcg acaaatcctt
     1321 ttactattga caatcctaat cctgttccac cggcagccga atattttacc cgataaaatt
     1381 tgtcaaaaac tttttcaatt gcttcttcag gaaatccatt gccattatcc tgcactatta
     1441 atacaagatg ttcttcctga gaaaatgcct taatcgtaat aacggcatgt ttaggtgtat
     1501 atacagccgc attcacagcg aggttataaa gtatttgttc tataagtcca tgatcaattt
     1561 taaataaggg aacatcatct tttatactta caataaatgg atgagtagca taatcatctt
     1621 ttaatcgatt tacaacttcg taggcaattt cgttaatgtc gcaccaatcg cgtttaggtg
     1681 caataacacc ggattcgagt cgcgacatat taagtaaatt ttccacctgt ctgtttaatc
     1741 gccaagcagc ctgcgcaatt tctgttgtta attcttttct attggtttcg gtaagtttga
     1801 tattttctgt ttgcagatta tccgtagcgc caataatggt agcaattggt gtttttaatt
     1861 catgggataa tgaattgagt aaggtattat acaatttcaa ggtgttctcc tctccttcct
     1921 tttgttgagt ttgtctttcg gtagttctga ttttgttgg
//
LOCUS       DAORKW010000649         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3762_length_1957_cov_3.466351, whole genome shotgun sequence.
ACCESSION   DAORKW010000649 DAORKW010000000
VERSION     DAORKW010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3762_length_1957_cov_3.466351"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..571)
                     /locus_tag="PLB46_16545"
     CDS             complement(107..571)
                     /locus_tag="PLB46_16545"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF78190.1"
                     /translation="MSEVKHLKKLFADLYDGVPWTEVKLTTIITDITSEQAAKKIIPE
                     ANSIWQLVQHCVGWRDNVLRKLQGEIFNSPEHNYLLPPDNVSPQAWQHLLGRLEQSEK
                     NWEQFLEQLDDNKLHEPYLPSKGKFTIYEVIHGILHHDNYHFGQIVMLKKLL"
     gene            complement(568..1095)
                     /locus_tag="PLB46_16550"
     CDS             complement(568..1095)
                     /locus_tag="PLB46_16550"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF78191.1"
                     /translation="MTRIEVTKKIEISIKLMSDTLSQFSSEPFFTRKNDKWSPAEQAQ
                     HLVLSIKPLQLAYRLPGFALRLLFGKMNRPTRTYEELLDKYHAKLKTGATASAAYVPK
                     QLKTGTTPEAVIASLQTAHQQLLKKVENWTESDLDKYVLPHPLLGKISLREMLYFTDF
                     HILHHNSLTKTIYLS"
     gene            1212..>1957
                     /locus_tag="PLB46_16555"
     CDS             1212..>1957
                     /locus_tag="PLB46_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78192.1"
                     /translation="MLRIRLMLCAVLLLLGVLTHAQTNISGVINSYWEVTGIDKCNNN
                     VTLPVTPIGLAAGDHVILIQMRGVDAEADNSPAYGSIINLSKSGNYEMFTVQSVVFNV
                     VTFNEVVGRLYQLAGRVQLVRVPEYNDAKVVGEVTGQPWNGTTGGVIAMIVNGTLTLN
                     ENIDAKNIGFRGADVTINTPCLVGGPDGFNGYVTTLPEDKAGKKGEGISENGDNFYAR
                     GAPANGGGGGNDRQTGGGGGCAWRRCRQWR"
BASE COUNT          570 a          334 c          424 g          629 t
ORIGIN      
        1 caggattacc accaaaaacg gtatctgtaa atgcatcaat aagataaata ggcaggcgca
       61 taaaattggt aggtgttttt atttgatgct gtaaatgtaa acattctcac aataatttct
      121 ttaacataac aatttgtcca aaatgataat tgtcgtggtg gagaatacca tgtatcactt
      181 cataaatagt aaatttccct ttagaaggta aataaggttc gtggagttta ttatcgtcta
      241 gttgttccag gaattgttcc caattttttt ccgactgttc tagccggccc aataagtgtt
      301 gccatgcctg aggtgaaacg ttatcgggtg gtaacaggta attatgttcc ggcgaattaa
      361 atatttcacc ctgcagtttt cgtaaaacat tatcgcgcca gccaacacaa tgctgcacta
      421 attgccatat agaatttgct tcaggtataa tttttttcgc agcctgttca gatgtaatat
      481 cggttataat tgttgtgagt tttacttcag tccaaggtac gccatcatat aaatcggcaa
      541 ataatttttt tagatgtttt acttcactca tgataaataa attgtttttg ttaagctgtt
      601 atgatgtaag atatgaaaat cggtaaaata taacatttca cgtaatgaaa ttttacctaa
      661 taaagggtgc ggtaatacat atttatcaag gtctgattct gtccaatttt ctactttttt
      721 taacaattgt tgatgtgcag tttgaagcga tgcaataacg gcttcaggtg ttgtaccggt
      781 ttttaattgt ttaggcacat aagctgcgga tgctgttgcc cctgttttca attttgcatg
      841 gtatttatct agcaattctt catatgttct cgttggtctg ttcattttgc cgaatagtaa
      901 ccgcagcgca aaaccgggta aacggtaggc caattgcagc ggttttatgg ataaaaccag
      961 gtgttgggct tgttcagcag ggctccattt gtcgtttttc cgggtaaaaa atggttctga
     1021 agaaaattgg ctaagtgtat cactcatcaa ttttatgcta atttctatct tttttgtgac
     1081 ctcaattctt gtcatatggt aagtatttgt gaataaaatg ctctatcaaa ttcaggtcaa
     1141 attaactcaa aatccccgaa atttgtggtt attatgtatc ttcgtccaaa attcacacac
     1201 atactactac tatgttaaga atcaggttaa tgttatgtgc tgtgttactc ctcctcggcg
     1261 tgttaacgca tgctcaaacg aatatttccg gggtaatcaa cagttactgg gaagttacag
     1321 gaatcgacaa atgtaataat aacgttacat tacctgttac cccaattggg cttgctgcgg
     1381 gagatcatgt aatactcatt caaatgcgtg gagttgatgc tgaagctgat aattcacctg
     1441 cttacggttc cattataaat ctttcaaaaa gcggtaatta tgagatgttt accgtgcagt
     1501 cagtggtgtt taacgtagtt acctttaacg aagtagtagg cagattatat caacttgccg
     1561 ggcgagttca attggtaagg gttccggaat ataatgatgc taaagtagta ggtgaagtaa
     1621 cgggccaacc atggaatggc acaactggtg gagttattgc catgattgtg aatggaactt
     1681 taactttaaa cgaaaatatt gatgcaaaaa atataggttt cagaggagca gatgtaacca
     1741 tcaacacacc atgtttagtt ggaggacctg acggttttaa cggatatgtg acaaccttac
     1801 ctgaagataa agcgggtaaa aagggtgaag gtatttctga aaatggagat aacttttatg
     1861 cacgtggggc tcctgcaaac ggcggtggcg gaggaaacga ccgtcaaacc ggcggcggcg
     1921 gtggatgtgc gtggcggcga tgccggcagt ggcgcag
//
LOCUS       DAORKW010000650         1956 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3767_length_1956_cov_3.281957, whole genome shotgun sequence.
ACCESSION   DAORKW010000650 DAORKW010000000
VERSION     DAORKW010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3767_length_1956_cov_3.281957"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PLB46_16560"
     CDS             <1..568
                     /locus_tag="PLB46_16560"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF78193.1"
                     /translation="VALAELDALFDGAATANLADSFISEFGKDPFVKGAYSFPAPNTY
                     FTETDTTRIDLGLPVDCKLFFAGEATNNNHPSTVHGALESGARAAAEVLECFTLPIDE
                     NVVTDNINMYVFNNNAYFTINTAVIATSVLSLHSIDGKKIQQFYYGRVPAGVNEFQFE
                     ISNIANGIYILSADINGKKHQVKVEIIN"
     gene            855..1235
                     /gene="gcvH"
                     /locus_tag="PLB46_16565"
     CDS             855..1235
                     /gene="gcvH"
                     /locus_tag="PLB46_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRF78194.1"
                     /translation="MNFPENLKYTKDHEWVLIEGNVGTIGITDFAQRELGDIVYVEID
                     GVGSHMDKEATFGTVEAVKTVSELFMPVSGTITEMNADLDSKPESVNQDPYGSGWMIK
                     VELSNPSEIDGLMTAAQYNELVSA"
     gene            1239..1607
                     /locus_tag="PLB46_16570"
     CDS             1239..1607
                     /locus_tag="PLB46_16570"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRF78195.1"
                     /translation="MNSFRRYAPYLAALWALIILVVSVIPGEDLPSLSIWEPDKVLHA
                     FVYGVLTALIYITRPQNAVFIKKLVFQAILLCILYGFFIELIQLVLPTRKFDILDALA
                     NTVGCFIAGGIILLISRKRA"
     gene            1697..>1956
                     /locus_tag="PLB46_16575"
     CDS             1697..>1956
                     /locus_tag="PLB46_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78196.1"
                     /translation="MDNIDPYLIKSFDDIVFEHRNKQYGSYYLRSITKKNTLRGLSAT
                     TVLVGLALLFHYVDFNLFKHETPPLVLYETEVTLSDPPPILET"
BASE COUNT          615 a          354 c          358 g          629 t
ORIGIN      
        1 tgttgctctt gccgaactcg atgccttatt tgatggtgct gccactgcca atttggcaga
       61 tagttttata tccgagtttg gcaaagaccc ttttgtaaaa ggcgcctatt catttcctgc
      121 tccaaatacc tatttcacag aaactgatac aacccgcatt gatttaggct taccggtaga
      181 ttgcaaattg ttttttgcag gtgaagccac caacaacaat cacccttcca ccgtacatgg
      241 tgcattggaa agtggagcac gcgcagcagc tgaagtatta gaatgtttta cgttaccaat
      301 tgatgaaaat gttgtaaccg acaatataaa tatgtatgta tttaataata acgcatattt
      361 tactattaac acagcggtta ttgcaacttc tgtattgtcg ttacacagta tagatggtaa
      421 aaaaatacag cagttttatt acggccgagt acctgcaggt gtaaatgaat tccaatttga
      481 aattagcaac atcgcaaatg gtatatacat tttaagtgcc gacattaatg gaaaaaaaca
      541 tcaggtaaaa gttgaaataa taaactaact ccaaactaaa attattttaa aatactaagt
      601 acgaaatatt gcttgcaaca atccatatta tttgattgga gattttatta caattatacc
      661 atcttcacat ttataaaagc atcaacaggt taacaacaaa ccatttcggg ttatagcaaa
      721 ttattggtaa ttaaatcttg ccaatacact caaacactac atggctaatc tgcctttcaa
      781 caaactagca gtaatgctaa ttcggtttcg ccagttcagg caaatccatt acatttgtgt
      841 caaaatcaca agaaatgaat tttccggaaa atttaaaata caccaaagac cacgaatggg
      901 tgttaattga aggcaatgta ggcactattg gcattaccga ttttgcacaa cgcgaattag
      961 gtgatattgt atatgtggag attgatggcg ttggaagcca tatggacaaa gaagctactt
     1021 ttggtactgt tgaagcagtt aaaacagttt ctgaactttt tatgccggta tcgggcacaa
     1081 ttacagaaat gaatgctgac ttagacagca aacctgaatc agttaaccaa gacccttatg
     1141 gcagtggttg gatgattaaa gttgagcttt ccaacccttc agaaattgat ggattgatga
     1201 cagctgcaca atacaatgaa ctcgtttcag cttaataaat gaatagcttc cgcagatatg
     1261 ccccgtatct agcagcttta tgggctttaa ttattttggt agtttcggtt atccctggag
     1321 aggacttacc ttctttatct atttgggaac ctgacaaagt gctgcacgct tttgtttatg
     1381 gcgtgttaac agctttgatt tacataacac gcccccaaaa tgctgttttt atcaaaaaat
     1441 tggtttttca ggccatactt ttatgcattt tgtatggttt tttcatcgaa ttgatacagt
     1501 tagttttacc aacaagaaaa tttgacatac tagatgcatt ggctaataca gtcgggtgct
     1561 ttattgccgg aggtataatt ttgttaattt cccgtaaaag agcataaggg ctgaaggcaa
     1621 attgactatt ttcaaataaa attgtggcaa ttttcgtatt gggaatgttt tttgtctggt
     1681 aacagctgtt ataacaatgg ataatataga cccatatttg ataaagtcgt ttgatgatat
     1741 tgtttttgaa catcgcaata aacagtatgg gtcctattat ttgcggtcaa ttaccaaaaa
     1801 aaacacttta cgtggcttgt ctgcaactac tgttctggtt ggcttagcgt tactattcca
     1861 ttatgtcgac tttaacttat ttaagcatga gacccctcca ttggtgcttt acgaaacgga
     1921 agttacctta tctgaccctc ccccaatttt agaaac
//
LOCUS       DAORKW010000651         1947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3800_length_1947_cov_5.126850, whole genome shotgun sequence.
ACCESSION   DAORKW010000651 DAORKW010000000
VERSION     DAORKW010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3800_length_1947_cov_5.126850"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="PLB46_16580"
     CDS             <1..1187
                     /locus_tag="PLB46_16580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRF78197.1"
                     /translation="VKNEKALRGTVEFSFQAKNIGLGATGNDLICEIIGVNGEFIDPS
                     LIEMNSIQKWNNNSDVFSSTLLFSENFGLANYNWQTYTRNINFGAGYEYIIIKYYSEG
                     VKTGPAEEQGIDNVCLPCVCAIPQHLFEDELTASHAMLIWDNMGSEQYQLQYKTTVGG
                     AWTTVTVENTYHELSALLANTSYSWKVRALCDGVWTSYSAEKIFITPSAGTACTSPSV
                     LATSLITGTKATLTWNQIPAALQYQISYKKITDVTWTNIFTTSDSYMLSGLTPLTIYE
                     WKVKTQCAAGWKDFTSTSLFTTMALKEEAEQQFTSTEIVIYPNPVQDELNIQLPADIS
                     GDLNLVIIDLLGHVVVEQNIESGGGSNGIQLNISNIPTGVYLLTIYNQNIALATQKFI
                     KQ"
     gene            1369..1851
                     /locus_tag="PLB46_16585"
     CDS             1369..1851
                     /locus_tag="PLB46_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78198.1"
                     /translation="MKNYTLIAVFICLVTSLSAQTDNQAIGIRFGGGDGTESEISYQQ
                     YIGGPNRLEFDLGFFDNGWDEGFKFTLLYQLVGNIDGGLNWYLGAGGSIGGRDIDSND
                     KNWNDDSDDGLFLNVDGQLGIEYNFSIPLQLSLDARPEFGLINDDFDFGYGFGIRYTF
                     "
BASE COUNT          618 a          315 c          395 g          619 t
ORIGIN      
        1 ttgtaaaaaa tgaaaaagcg ctcagaggaa ctgttgaatt ttcttttcag gctaaaaata
       61 ttggccttgg tgcaactggc aatgatttga tttgtgagat aattggcgta aacggagaat
      121 ttattgatcc ttccttaatt gaaatgaatt ccattcaaaa atggaacaat aactcggatg
      181 tattttcatc aacattatta ttcagcgaaa attttggatt ggcgaattat aactggcaaa
      241 catatacccg aaatatcaat ttcggagccg gttatgaata catcattatt aaatattatt
      301 cagaaggtgt taaaaccggc ccggctgaag agcagggaat tgataatgta tgtttacctt
      361 gtgtttgtgc tattcctcaa catttatttg aagatgaact cactgcatct catgctatgt
      421 tgatttggga taatatggga agtgaacaat atcagttgca atataaaacc actgttggtg
      481 gtgcatggac aactgtgact gtagaaaata cgtaccatga attatctgct ttattagcaa
      541 atacttctta ctcatggaaa gttcgggcat tgtgcgatgg cgtatggaca agttattctg
      601 cggaaaaaat atttattaca ccaagcgcag gaactgcctg cacatcacca agtgttttag
      661 ctacatcatt aattaccgga acaaaggcaa cacttacctg gaaccaaatt cctgcagcgt
      721 tgcaatatca aatttcgtat aaaaaaatta cggatgttac ctggaccaac attttcacca
      781 cctcagatag ttatatgtta agtggtttaa cgccacttac catttacgaa tggaaagtaa
      841 aaacacaatg tgccgcaggt tggaaagatt ttacttctac ttcgttattc accactatgg
      901 cattaaaaga agaagctgaa cagcagttta cttcaactga aattgttatt tatcccaacc
      961 ctgtgcagga tgaattaaat attcagttgc cggcggatat ttcgggagat ttaaatttgg
     1021 tgattatcga tttattggga catgttgtgg tagaacaaaa cattgaatcc ggcggcggct
     1081 ctaatggaat tcaattaaat atttcgaata ttccgacagg tgtttatttg ttaaccattt
     1141 acaaccaaaa catcgctctg gcgacacaaa aatttattaa acaataacag gttcatgaat
     1201 gaataatagt aacccggact tttagttccg ggtttttttg tgccacaaca ggtacaaatt
     1261 gtggcaacta tcctcaattt tcaggtaata taggtggaaa ttgagccaaa tcaggtcatt
     1321 tgctcaagtg gctccgtatt tgcactactt atatcaaaat cttaaataat gaaaaattac
     1381 acattaatag cagtatttat ttgtttagta acaagtttat ctgcgcaaac tgataatcag
     1441 gcaattggta ttcgatttgg tggaggtgat ggcacggagt ccgaaatttc ataccaacaa
     1501 tatatcggag gaccaaaccg ccttgaattt gacctgggtt tttttgataa cggctgggat
     1561 gaaggattta aattcacttt gttatatcaa ttagttggta acatagatgg tggattaaac
     1621 tggtatttag gtgcaggtgg tagtattgga ggccgcgata ttgattcaaa tgataaaaat
     1681 tggaacgacg attcagatga tggtttgttt ttgaatgtag acgggcaatt agggattgaa
     1741 tataattttt ccattcctct tcaattgagc cttgatgcac gtcctgaatt tggacttata
     1801 aacgatgact ttgatttcgg atatggtttc ggtatcagat atacatttta gtttttattg
     1861 aagattgaat gtaaagccgg agatgatact ccggcttttt tatttaggca acaaaaccac
     1921 cgtcgtagac ggtggtcagg agtaaag
//
LOCUS       DAORKW010000652         1946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3802_length_1946_cov_4.025383, whole genome shotgun sequence.
ACCESSION   DAORKW010000652 DAORKW010000000
VERSION     DAORKW010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3802_length_1946_cov_4.025383"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1323
                     /locus_tag="PLB46_16590"
     CDS             58..1323
                     /locus_tag="PLB46_16590"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRF78199.1"
                     /translation="MNEAFLQFIWKMKLFTTTGIYTTEGEPVTIIHNGEHNTHSGPDF
                     TNARIKIGETTWAGNVELHIRSSEWNEHKHSSDRAYDNVILHVVYVHDDKKSLMPTLE
                     LNNLIDNDLIQHYKFMMQTAAWIPCEKSIHQVEEIVVKQQLNRLLSERLEQKALHVEN
                     RLLVNNNDWEATCYQLIARSFGTNINADPFEGVARSLPYKTILKHLNQPKQIEALLFG
                     QAGFLEGSFREIYPHQLQAEYKFLKTKYQLQGIRPLEWKFLRMRPANFPTIRMSQLAA
                     FLATHDRIFSHIIHAPDSNTIKQLFRAEASPYWKEHYHFKKAAAVKSATLGKDTINLI
                     LINTIAPLLFLYGKKMGDEQFCIKAVDLLEHLDPENNTITRQWATLGLTAKHAGDSQA
                     LLELKKQYCSNKRCLECSVGYRILKTGKK"
     gene            1347..1568
                     /locus_tag="PLB46_16595"
     CDS             1347..1568
                     /locus_tag="PLB46_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HRF78200.1"
                     /translation="MLYRIKHLIETGAFGACAWMGEKMGIASSRVRLFFVYASFLALG
                     SPVIIYMIIAFWVNLKNYLRDKIDPIRDL"
     gene            complement(1637..1720)
                     /locus_tag="PLB46_16600"
     tRNA            complement(1637..1720)
                     /locus_tag="PLB46_16600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1684..1686),aa:Leu,seq:gag)
BASE COUNT          621 a          336 c          433 g          556 t
ORIGIN      
        1 atttcattat cgcaagataa ttaaaaattt attgttttac ccttaaaaat ttattttatg
       61 aacgaagcat ttctccagtt tatctggaag atgaagttgt ttaccactac cggtatttac
      121 acaactgaag gtgagcctgt caccattatt cacaacggcg aacacaatac acatagtggt
      181 ccggatttta ctaatgcacg cataaaaatt ggcgaaacca cctgggcagg gaatgtggaa
      241 ttgcatattc gctcctcgga gtggaatgag cacaaacaca gcagcgacag ggcttacgac
      301 aatgtgatat tacatgttgt gtatgtgcac gatgataaaa aaagtttaat gcccacattg
      361 gaattaaaca atttaattga caatgattta attcagcatt ataaatttat gatgcaaact
      421 gcagcatgga ttccttgtga aaaaagtatt catcaagtgg aggaaattgt tgtcaagcaa
      481 caacttaacc gcttactatc ggaaaggttg gaacaaaaag cattacacgt tgaaaataga
      541 ttattagtaa ataataacga ttgggaagca acctgttatc aattaattgc acgcagtttt
      601 ggcaccaata ttaatgcaga cccttttgaa ggtgtggcga gaagtttacc ctataaaacg
      661 attttaaaac atttaaatca gcccaaacaa attgaagcat tattatttgg tcaggctggt
      721 tttttagaag gttcatttcg tgaaatatat ccgcatcaat tacaagctga atataaattt
      781 ctgaaaacga aatatcagtt acaaggtatt cgaccattgg aatggaagtt tttgcgtatg
      841 cggcccgcca attttccgac catcagaatg agtcagctgg ctgcattttt ggcaacacat
      901 gacaggatat tttcacatat cattcatgcg cctgacagca ataccatcaa acaattgttt
      961 cgtgcggaag ctagtcccta ctggaaagaa cattatcatt ttaaaaaagc agccgcagta
     1021 aaatcggcaa cgttaggcaa ggacacgatt aatttaattc tgataaatac gattgcccct
     1081 ctcctgtttt tgtatggcaa aaaaatgggt gatgaacaat tttgtataaa agctgttgat
     1141 ttgctcgaac atttagaccc ggaaaataat acgataacca gacaatgggc aacgttaggt
     1201 ttaaccgcaa aacacgcagg agatagtcag gcgctgctgg aattaaaaaa acaatattgc
     1261 agcaataaac gctgtttgga atgtagtgtg ggttaccgaa ttttgaaaac tgggaaaaaa
     1321 tgataaattt gtaaaaacaa ccaacaatgc tatacagaat taaacacctt attgagaccg
     1381 gcgcctttgg tgcatgtgcc tggatgggag aaaaaatggg cattgcttca agtcgtgtgc
     1441 gcttattttt tgtttatgct tcatttttag cattggggtc gccggtgatt atttatatga
     1501 taattgcttt ttgggtaaat cttaaaaatt acctgcgcga taaaattgat ccgattcgtg
     1561 atttataata ggtaagtctg cctaattatt tgttaatcag aatattatag aaaattttgt
     1621 tttgggaggt aagaagtgcc caggacaaga ttcgaacttg cacacccttg cgggcgctag
     1681 cccctcaagc tagtgtgtct accaattcca ccacctgggc aaggcgggtg caaaggtaat
     1741 acgaattgtg gaatgatgca agggaacctg cctggcggca gacaggcgcg gatgacgcgg
     1801 atgtattggg tttgtatttt gtttggcgga tgaacgcgga ttactttgga ggtggaatgg
     1861 aatgaataac gctgtagtgg gatgcgggtg aaagggaacc tgcctggcgg cagacaggcg
     1921 cggatgacgc ggatgtattg ggtttg
//
LOCUS       DAORKW010000653         1936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3838_length_1936_cov_3.651249, whole genome shotgun sequence.
ACCESSION   DAORKW010000653 DAORKW010000000
VERSION     DAORKW010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3838_length_1936_cov_3.651249"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLB46_16605"
     CDS             <1..307
                     /locus_tag="PLB46_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRF78201.1"
                     /translation="KPGGDIVQNKKTFLVLKALNIASAEDKNLLSKLYTDKSIESDKK
                     IEPVLTIFNKYDIRQLAEDNMEQYYQSAIEAFEAVNVPGDRKVALAGLIESVFKRTH"
     gene            complement(304..936)
                     /locus_tag="PLB46_16610"
     CDS             complement(304..936)
                     /locus_tag="PLB46_16610"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRF78202.1"
                     /translation="MIKGFSHRLYQLLTGDLPGVLAHQVMAPVTRPLKAFDLSDYPDA
                     KVAAVMILLYPKQDTIFLALIKRPDYSGVHSGQVSFPGGKSEESDADYQFTALRETEE
                     EIGVKASLIQVIGQLSNVYIPPSNFFVFPFVGFLDHHPQFLPDANEVDYVIEVPVDIL
                     LDPSIKSTVKIERPEVSFEAPCYLINGHTVWGATAIILSEFEFLLKQMDL"
     gene            1012..>1936
                     /locus_tag="PLB46_16615"
     CDS             1012..>1936
                     /locus_tag="PLB46_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78203.1"
                     /translation="MKKSILLLGFISTITTIFAGNHAKTGDIQYIQNNGQWASHILYS
                     TPVYGGNLFLEQNCFTYYFSNMSEIGELHHSGNKNQLENFIIKKHAFKVSFVGANPNI
                     TLSGENKFANYYNYFLGNDQSKWQGKVPAYGAIRYPQLYDGIDMLVYSQSSSVKYDYI
                     VEPGADANKIKVLYDGLDNIQLVDGNLELTTSINKMIELKPVAYQMINGSKVDVLCEY
                     LLDVTTISFSFPNGYDKNYELVIDPATLIFASYTGSSADNWGNSATFDNEGFLYGAGM
                     TFGDGYPLTIGAYQTTWGGGGGFYVGDITLSK"
BASE COUNT          634 a          361 c          359 g          582 t
ORIGIN      
        1 aaaaccgggt ggtgatatcg tgcagaacaa aaaaacattt ttagtgctca aagcattaaa
       61 tattgccagt gctgaagata aaaatttatt gtcgaaatta tacaccgata aatccataga
      121 aagtgataaa aaaatcgaac cggtgcttac tatttttaac aaatacgata tcaggcaatt
      181 agctgaggat aatatggaac aatactacca gtcagcaatt gaagcctttg aagcagttaa
      241 tgttccaggc gatcgcaaag ttgctttggc aggattaatt gaaagcgttt tcaaacggac
      301 gcattaaagg tccatttgtt ttaacaaaaa ctcgaattca cttaaaataa tagcagttgc
      361 cccccaaacg gtatggccat taatcaggta acaaggtgct tcaaatgaaa cttcaggccg
      421 ttcaattttt acggtagatt taatagaagg gtccaacaaa atatcaaccg gaacctcaat
      481 tacatagtcc acttcgttgg catcaggtaa aaattgagga tgatggtcca aaaaaccgac
      541 aaatgggaaa acaaaaaaat tgctgggtgg tatatacaca ttacttagct gtccaatcac
      601 ctgaattagc gaagctttca caccaatttc ttcctctgtt tcccgcaaag ccgtaaattg
      661 gtaatctgca tccgattctt ccgatttacc acccggaaaa cttacttgtc cgctatgcac
      721 tccactataa tcaggtcgtt taatcagtgc taaaaagata gtatcctgtt taggatataa
      781 taaaatcata acagcagcca cttttgcatc tggataatcg ctcagatcaa aagctttcaa
      841 aggcctggta acgggagcca ttacctgatg cgcgagcaca ccgggtaaat cgccggttaa
      901 caattgatat aatctgtgtg aaaatccttt tatcatattg aagcaataat tacatttgat
      961 gcgccaaaat atcctatttt tttataaatt agtctcaaat attccttttg catgaaaaaa
     1021 tcaattttac tccttggttt tatatccacc ataaccacca tttttgcagg aaatcatgcc
     1081 aaaaccggcg acattcaata catccaaaat aatgggcaat gggcaagtca tatcctgtat
     1141 tctactcctg tttacggggg taatttattt cttgagcaaa attgttttac ctattacttt
     1201 tcgaatatgt ctgagattgg agaattacat cactcaggca ataaaaatca actggaaaat
     1261 tttatcatta aaaaacacgc tttcaaagtg tcatttgttg gcgcaaatcc aaacatcact
     1321 ttaagtggag agaacaaatt tgccaactat tacaattact ttcttggtaa tgaccaatcc
     1381 aaatggcagg gaaaagttcc ggcttatggc gcaatacggt atcctcaact ttatgatggt
     1441 atcgacatgc tggtatatag ccagagtagt tctgttaaat atgattatat cgttgaaccg
     1501 ggtgctgatg ctaacaaaat taaagtgttg tatgatggtt tggacaatat ccaactggtg
     1561 gatggcaatc tcgaattaac aaccagtatc aacaaaatga ttgaacttaa gcctgttgcc
     1621 tatcaaatga ttaacggctc aaaagtagat gtgttatgtg aatacttact tgatgtaacc
     1681 actatatctt tctcgtttcc aaatggttat gataaaaatt acgagttagt tattgaccct
     1741 gctactttga tatttgccag ttatacaggt tcttcagccg ataactgggg gaattcggca
     1801 acgtttgata atgagggttt tttatatggt gccggaatga catttggtga tgggtatcca
     1861 ctaactatag gtgcttacca aactacttgg ggcggaggcg gtggctttta tgttggtgat
     1921 ataacacttt ccaaat
//
LOCUS       DAORKW010000654         1934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3843_length_1934_cov_4.271953, whole genome shotgun sequence.
ACCESSION   DAORKW010000654 DAORKW010000000
VERSION     DAORKW010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3843_length_1934_cov_4.271953"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..610)
                     /locus_tag="PLB46_16620"
     CDS             complement(140..610)
                     /locus_tag="PLB46_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5362 family protein"
                     /protein_id="HRF78204.1"
                     /translation="METLDNNLSNGENLVINGQIKSHLLETAKWGRILAILGFVGLGL
                     MIILLIFGVSMFNTILSQQPMQSGPSLGGGSMTLSFVFFALFMLIYIFPLLYLYKGSV
                     AYIKALNNDNQEELEAGFENYKSLFKFIGIFTIIVFGLYLLIFIGSILLGGLLS"
     gene            complement(639..974)
                     /locus_tag="PLB46_16625"
     CDS             complement(639..974)
                     /locus_tag="PLB46_16625"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRF78205.1"
                     /translation="MPYKEKEIEKIYWSIGEVSEMLDLSFAQIRLWEKEFDVFNLKKN
                     KKGDRYFTKKDIENLRVIKYLLKEKKYTIKGAREKMKSGGDKADNQYQAVAALKKIRQ
                     FLVELKEQL"
     gene            complement(1065..>1934)
                     /locus_tag="PLB46_16630"
     CDS             complement(1065..>1934)
                     /locus_tag="PLB46_16630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRF78206.1"
                     /translation="TAGVFASLIVWIAYTYFDSPKERYLKDQISQMELEFDFMNNKLD
                     TMGVILGELAERDDNIYRVIFEAEPVSRSERIAGIGGSDRFKRMNKLDNAEMLKNTAM
                     KLELIRRQMVVQSKSFDEITNLVQNKEKLLAHTPSIQPVSNKDLERIASGFGWRIHPI
                     YKFAKFHEGLDFTAPKGTDVFATADGTVVQSDNNSSGYGNVIVIDHGYGYKTRYAHLS
                     AFKVRAGQKVKRGELIGLVGNSGLSTAPHLHYEVEKNNERVDPINFFYTDLTAEEYAR
                     MIELANKSNQSYD"
BASE COUNT          593 a          390 c          327 g          624 t
ORIGIN      
        1 cgcccatcaa taaaacgact ggttgtaact atttctttgt atttaacatc gtcttcaaaa
       61 gcaaatagtt tagtatgtat acaatttgta agtaccaata ctaaaaccaa ccggaaaaaa
      121 tatttgtttt gtttcatatt caacttaata atccgcccaa taaaatactt ccaataaaaa
      181 taagcaggta taaaccaaaa acaattatag taaagatgcc gataaatttg aacagcgatt
      241 tataattttc aaaaccagcc tcgagttctt cctgattatc attattgagt gctttgatat
      301 aggcaacaga acctttgtat aaatacaata atggaaaaat gtaaatgagc ataaaaagtg
      361 cgaaaaatac aaaggataaa gtcattgaac caccccctaa acttggacct gattgcatgg
      421 gctgttgact caaaatggta ttaaacatac tcacgccaaa aattaataaa ataatcatta
      481 agcctaagcc tacaaacccc aatatagcca gtattctgcc ccatttagca gtttccagta
      541 aatgcgattt tatttgtccg tttataacca ggttttcacc attactcagg ttgttgtcaa
      601 gtgtttccat aaaaataatt ttacaataaa taaattgttt acaattgttc tttcagttca
      661 accaaaaatt gtctgatttt tttcaaagct gcaacagcct gatattgatt atccgcttta
      721 tcgccacccg atttcatttt ttcgcgagca cctttaatgg tgtatttttt ttctttgagc
      781 agatatttga ttacgcgtaa attttcgata tcttttttgg taaaatatct gtcgcctttt
      841 ttatttttct tgagattaaa aacatcaaat tctttttccc ataatcgtat ttgagcaaag
      901 ctgagatcca gcatttctga aacttcccct atcgaccaat aaattttttc tatttccttt
      961 tctttataag gcatgatgag gcaataagtg tgtgcataat caattgaatt acaaatatat
     1021 caattttatc ttatacaata gcacatgagg tagcggctgt aaaattagtc gtacgactgg
     1081 ttggatttgt ttgccagttc tatcatacgg gcatattctt ctgcagtgag gtcggtatag
     1141 aagaagttaa tagggtcaac tctttcgttg tttttttcca cttcataatg caagtgaggg
     1201 gcagtagaca agccggagtt acctaccaaa ccaattaact cgccgcgttt taccttttgc
     1261 cctgcgcgca ctttaaaagc gcttaaatgc gcataacggg ttttatagcc atagccatgg
     1321 tcaataacta tcacattacc atatccactg gagttattat cggactgcac aacagtgcca
     1381 tctgcggtag caaaaacatc ggttcctttt ggagcggtaa agtctaaacc ctcatggaat
     1441 ttggcaaact tataaatagg gtgaattcgc cagccaaaac cggaggcaat tctttccagg
     1501 tctttattac tgacaggctg aatagacggc gtatgtgcaa gcagtttttc tttgttttga
     1561 acgaggttgg ttatctcatc aaacgactta gattgcacca ccatttggcg gcgaatcaac
     1621 tcgagtttca ttgccgtgtt ttttaacatt tcggcattgt cgagtttgtt catacgttta
     1681 aaccggtcgc tacccccaat tcccgcaata cgctcactac gtgaaaccgg ctctgcttca
     1741 aatattaccc tgtaaatatt gtcgtccctt tcggcaagtt ctcccaaaat gacccccatg
     1801 gtgtcgagct tgttgttcat gaagtcgaac tccagctcca tttgagaaat ctggtctttc
     1861 aaatagcgct ctttaggtga atcaaagtag gtataggcaa tccaaacgat taaagaggca
     1921 aaaacaccgg ctgt
//
LOCUS       DAORKW010000655         1934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3844_length_1934_cov_3.864822, whole genome shotgun sequence.
ACCESSION   DAORKW010000655 DAORKW010000000
VERSION     DAORKW010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3844_length_1934_cov_3.864822"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLB46_16635"
     CDS             complement(<1..612)
                     /locus_tag="PLB46_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HRF78207.1"
                     /translation="MIIRSKAPLRIGLAGGGTDVSPYSDIYGGCILNATINKYAYATI
                     IPRRDNKIILRAMDRNESIELESESKLAIDGKLDLLKGVYNRVVKDFNHNAPLSFELN
                     TYVDAPPGSGLGSSSTLVVAIVGAFTEWLGLPLGEYDIAHLAYDIERKDLAMAGGKQD
                     QYAATFGGWNFMEFYKDDKVIVNPLRIRPEYLQELSFNIVMYYT"
     gene            878..>1934
                     /locus_tag="PLB46_16640"
     CDS             878..>1934
                     /locus_tag="PLB46_16640"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HRF78208.1"
                     /translation="MSIKSLLTTSVLFLCCCLNVYSQWTQMVFPDTNNVKAITFTNAS
                     TGFCTGQYFDFALGDYIGIIYKTTDAGLSWDTVLNTGLGNNYANDITFFNENIGYTSN
                     RNANFFRTTDGGASWDTLPFAGDNALWTAITLIDANSGYSCGFSGEIIKFEDAGETVT
                     TLYSSGVVMEICRDMICLSSDTCYSLWQNEILTTTNGGTTWQTILTSDTTGNTSFYVF
                     DDGTIVVFTKYESITCLRSTNNGASWEITPITATTAAINKVTFFEETGYAVSADGIIL
                     QSIDSGRTWNAVPVITPSGENPAALNDIYLFNAYTGFICGDNGTLLSLGLPTAISSTI
                     ATVINITPNPSNNLITIT"
BASE COUNT          593 a          372 c          371 g          598 t
ORIGIN      
        1 ggtgtaatac attacgatat taaaagaaag ttcttgtaaa tattcagggc gaatgcggag
       61 cgggttaaca ataaccttat cgtctttata aaactccata aaattccagc caccaaatgt
      121 tgctgcatat tggtcctgct ttccgccggc catagccaaa tctttgcgtt cgatatcgta
      181 tgccaaatgg gcaatatcgt attcgccaag tggcaaaccc agccattcgg taaaggcccc
      241 aacaatggcc acaacaaggg tagatgatga gcctaagccc gagcccggag gcgcatctac
      301 ataagtattt aactcaaacg aaagcggagc attatggtta aagtctttca ctacgcggtt
      361 atacacgcct tttaagaggt cgagtttgcc gtcgatagcc agttttgatt cagattccag
      421 ctcaatagac tcattgcggt ccattgcgcg taaaataatt ttattgtctc tgcggggaat
      481 aatggtagcg taggcatact tattaatcgt cgcattcagg atacagccac cgtatatatc
      541 gctgtagggg ctcacatctg ttccgccgcc tgccagtcct atacgcaaag gtgctttact
      601 tcttataatc atttcgtgct taggttaaag ggttacttta aagattatct cctcaaatcg
      661 ggtgcaaagt tccataaaaa ctatgaaaaa accatgattt accctttttt agatatataa
      721 ctgttgggta gatagtgaaa agtgcgctga ggttgctttg ccgtttggat ataatatggc
      781 cggttttact ccacctgata aatggttttg ttgtccgcaa accatttttg tttacaataa
      841 tttaaaatac ctttattgct ccttattaaa tgaaaacatg agcattaaat ctttactcac
      901 tacaagtgtc ttgtttttgt gttgttgttt gaacgtttat tcgcaatgga cacaaatggt
      961 ttttcccgat acgaataatg taaaagccat cacttttacc aacgcttcaa ccggtttttg
     1021 cacaggccaa tattttgatt ttgcattagg tgattatatc ggcatcattt acaaaacaac
     1081 cgatgccggt ttaagttggg atacagtgct caatacagga ttaggtaata attatgcaaa
     1141 tgatattacc ttttttaatg aaaacattgg ctatacgagt aacaggaatg ccaatttttt
     1201 tcgcacaaca gatggcggtg cttcttggga tacccttcca tttgccggcg ataatgcatt
     1261 atggactgcc ataactttaa ttgatgccaa tagtggttat tcgtgtggat tttcaggaga
     1321 aataataaaa tttgaagacg caggtgaaac ggttaccaca ttatatagca gtggtgtagt
     1381 aatggaaata tgccgcgaca tgatttgttt atcttcagac acttgttata gtctttggca
     1441 aaatgaaata cttacaacaa caaatggagg aactacctgg caaactattt taacttcaga
     1501 tacaacaggt aataccagtt tttatgtgtt tgatgatggc actattgtag tttttaccaa
     1561 atatgaatct attacatgtt tgcgttcaac aaacaatggc gcaagttggg agattacacc
     1621 aataaccgca acaacagcag caattaacaa agtaaccttt tttgaagaaa caggatatgc
     1681 cgtatcggca gatggaataa tattacaaag tattgacagc ggtcgcacct ggaatgcagt
     1741 tccggtaata accccaagcg gcgaaaatcc tgcagcactc aacgatattt atttatttaa
     1801 tgcatataca ggatttattt gtggtgataa tggcacttta ctttctttag gattaccaac
     1861 tgccatttct tccacaatag caacagtaat taatataaca cctaatccaa gcaataattt
     1921 aataactatt acat
//
LOCUS       DAORKW010000656         1924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3864_length_1924_cov_3.936865, whole genome shotgun sequence.
ACCESSION   DAORKW010000656 DAORKW010000000
VERSION     DAORKW010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3864_length_1924_cov_3.936865"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="PLB46_16645"
     CDS             <1..1486
                     /locus_tag="PLB46_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRF78209.1"
                     /translation="LAPLRTGVVRELRQGVFDKILQLPLAYFSETRKGDIIARMSNDV
                     KEVESSMMNTIESTFRDPVTMLIFFIAMMTMSFELTIFVLILLPIAGFAIGRIGKSLK
                     RKSQKEQVQLGYLMSLFEESLSGLRIIKAFNAERYQKGKFLRENRELESLATKAVRRL
                     QVSSPLIEFLSVCVVAALLYYGGSIILDGNGQFSADYFLGYILVFANMLGPARNVANS
                     YVYIQRGIASLERIESIMDAEINIKESLHATNIKTFERAVEYKNVCFQYAEKPVLNGI
                     SLKIEKGKMIALVGPSGSGKSTFADLLARYHDVTSGEILIDDYNIRDLRISSLRNQLG
                     IVTQESILFNDTVFNNIAFGIDNALEEDVIKAAKVANAHDFIMRLENGYQTVIGDRGS
                     KLSGGERQRITIARAIYKNPPILILDEATSSLDTESEKLVQDALFKLMKNRTSIVIAH
                     RLSTIQFADEIIVMQEGRIVERGNHTALIAKNGLYAKLVEMQAF"
     gene            complement(1623..>1924)
                     /locus_tag="PLB46_16650"
     CDS             complement(1623..>1924)
                     /locus_tag="PLB46_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78210.1"
                     /translation="KATGYDSYFTMLNYEPYLMRMTDSYIINSGVDALKKIASDDDNF
                     WMSFSAMNILSGLIPQYQSALESNTDPQIQKSLQASIDYITAVLKELNTSEEGDY"
BASE COUNT          614 a          341 c          384 g          585 t
ORIGIN      
        1 cctcgcacca cttagaaccg gtgtagtacg cgaattgaga caaggggtat ttgacaaaat
       61 ccttcaactt ccgctcgctt acttcagtga aacccgaaaa ggcgatatca tagcccgcat
      121 gagcaacgat gttaaagagg tggaaagtag tatgatgaat accatcgaat ctaccttccg
      181 cgacccggtt actatgctca tattttttat tgccatgatg acaatgagct tcgagttgac
      241 catttttgtg ctcattttgt tacccattgc cgggtttgcc attggcagaa ttggcaagtc
      301 gctgaaacgc aaatcgcaaa aagaacaagt gcaactgggt tatttgatgt cgcttttcga
      361 agaatcgctt tcaggcctga gaattattaa agcatttaat gctgaacggt accaaaaagg
      421 taaattttta cgtgaaaaca gagaattgga atccctggca acaaaagctg tgcgtagact
      481 ccaggtttcc agtccgctta tcgaattttt aagtgtttgt gttgttgctg cattattata
      541 ttatggtggt agcattattt tagatggtaa cggacaattt tcagcagatt attttttagg
      601 ctatatttta gtttttgcaa atatgttagg gccggcaaga aatgtggcta actcttatgt
      661 ttacattcaa cgtggaattg catcactcga acgcatcgaa agtattatgg atgcagaaat
      721 taatattaaa gaaagtttgc acgctaccaa tatcaaaaca tttgagcgtg cagtagaata
      781 taaaaatgtg tgttttcaat atgcagaaaa accggtttta aacggcatta gtctgaaaat
      841 tgaaaaaggt aaaatgattg ccctggtagg accttccggc tcaggaaaat ctaccttcgc
      901 cgacttgctt gcacgatacc acgatgtaac atcaggcgaa atattaattg atgattataa
      961 tattcgcgat ttacggataa gcagtctgcg caatcaactc ggcatagtta cgcaagagtc
     1021 gattttattt aatgataccg tgtttaataa tatagcattt ggtatcgata acgcattgga
     1081 agaggatgta attaaagcag caaaagtggc caatgcacat gactttatta tgcgattgga
     1141 aaatggttat caaacagtta ttggcgacag aggcagtaaa ctaagcggtg gcgaacgtca
     1201 gcgaattacc attgccagag caatttataa aaatccccca attttaatat tagatgaagc
     1261 tacttcttca ttagatacag aaagcgaaaa attagtacag gatgcactct ttaaattaat
     1321 gaagaacaga acatccatcg ttattgcgca ccgcctttca accattcaat ttgccgacga
     1381 aattattgtt atgcaggaag gtcgaattgt tgagcgtggc aatcatactg ccctcattgc
     1441 caaaaacggc ttatatgcga agctcgttga aatgcaggcg ttttaaagta tgcgataaag
     1501 ggcttactat tttcaaaagc attactttaa tttgattttt tttgattagt tcagactaaa
     1561 aagatatcat aaaaaaaacg cccatcaacc gatgagcgtt tttaaagaaa gaactttatc
     1621 ttttaataat caccttcttc gctggtattt aattctttta atacagcggt aatataatca
     1681 atagaggctt gtaacgattt ttgaatttgc gggtctgtgt ttgattcaag tgccgactga
     1741 tactgtggga ttaaaccact aaggatattc attgctgaaa atgacatcca gaaattatca
     1801 tcatctgaag caatcttttt taatgcatca acacctgaat taataatgta tgaatcggtc
     1861 atacgcatta aatacggttc gtaattcaac atggtgaagt aactgtcata acctgttgcc
     1921 ttag
//
LOCUS       DAORKW010000657         1923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3868_length_1923_cov_4.453426, whole genome shotgun sequence.
ACCESSION   DAORKW010000657 DAORKW010000000
VERSION     DAORKW010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3868_length_1923_cov_4.453426"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLB46_16655"
     CDS             complement(<1..618)
                     /locus_tag="PLB46_16655"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78211.1"
                     /translation="MSNKPNSKHVLHFRLIILIGFIMAFSQKGFAQESDTTSLDSVKT
                     NTLFDFHTARVEYFTGNEINNFLPIDTSLHLFHRFNAARLNDFEMAYLGNLGAPAYAR
                     YYTFDRTLGFDYGRHERDTYMLSLDELKYYRCNVPFTNLYYVAGGQGEQVFHVTHTRN
                     FGKDFNFAIDFNKIISEGYFQYMNTDYTDVSLTAWYKTKNERYQVY"
     gene            complement(673..1497)
                     /locus_tag="PLB46_16660"
     CDS             complement(673..1497)
                     /locus_tag="PLB46_16660"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRF78212.1"
                     /translation="MERLMDRLNEAVHYIKQRTNFEPEYGIILGTGLGNLVSHIKIAH
                     EINYSLIPHFQVSTVESHSGKLIFGSIGDKKVVAMQGRFHYYEGYTMEEVVFPVRTMK
                     LLGIKKLFISNAAGSLNANINKGDLMIIRDHINLQPSNPLIGPNEYELGPRFPDLFKC
                     YDEQLINSAMDIAKANNITCHKGVYISVPGPNLETPAEYNYLHIIGGDAVGMSTVPEV
                     LAAVHMGLPVFAISVITDKGYPADEIEAVTVEDVIAVAKEAEPKMTLILQNLIASL"
     gene            complement(1544..>1923)
                     /locus_tag="PLB46_16665"
     CDS             complement(1544..>1923)
                     /locus_tag="PLB46_16665"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRF78213.1"
                     /translation="QPIVQIPFQQSVILMTGIAGNKQLKSYLSGKFSTIHIAAFSDHH
                     YFREKDFLRVAGSFPDTKTIITTEKDAMRLSEQKDILLQMGFSVFVLPVDVHFLGEEE
                     KFWNLITTFIDKYPDTNAEQANN"
BASE COUNT          600 a          363 c          333 g          627 t
ORIGIN      
        1 atatacctga tacctttcgt tttttgtttt ataccatgcc gttagagaaa catcggtata
       61 atcggtattc atatattgaa aatacccttc tgaaattatt ttattaaagt cgatagcgaa
      121 attaaaatct ttaccaaaat tgcgcgtatg tgtaacatga aacacctgct ccccttgtcc
      181 gcccgccaca taatataaat tcgtaaacgg cacattacaa cgataatatt ttaattcatc
      241 gagtgataac atataagtat cgcgctcatg tcgtccgtaa tcaaatccca atgttcggtc
      301 aaacgtataa tatctcgcgt atgccggagc gcctaaatta cccagatatg ccatctcaaa
      361 atcatttaac ctggccgcgt taaaacggtg aaaaagatgt aatgaagtat caataggtaa
      421 aaaattattt atttcgttgc cggtgaaata ttcaacacgt gcagtatgaa aatcgaaaag
      481 ggtatttgtt tttaccgaat ccaaagatgt agtgtccgat tcctgggcaa aacctttttg
      541 ggaaaatgcc ataataaacc caatcaaaat tatcaggcga aaatgcaata catgtttgct
      601 attgggttta ttactcacac aattatttta tacttctcgc atctgttata acaaaaatgt
      661 tgccgcaatt ttttaaagcg aagcaatcag attttgcaaa attaaagtca tttttggttc
      721 tgcctcttta gcaacggcaa ttacatcttc aacagtaact gcttcaattt cgtcggcagg
      781 ataaccttta tcggtaatta cggaaatggc aaaaacaggt aaacccatat gcactgccgc
      841 cagcacttca ggaacggtgc tcataccaac agcatctcca ccaataatat gtaaataatt
      901 atattctgca ggtgtttcca ggttgggccc gggaactgaa atatatacgc ctttgtgaca
      961 agtaatatta tttgctttcg caatgtccat ggcgctgtta attaattgct cgtcgtaaca
     1021 tttaaataaa tcggggaagc gcggacctaa ttcgtattca tttggaccaa ttaatggatt
     1081 acttggttgt aaattgatat ggtcgcgaat aatcattaaa tcacctttat tgatattggc
     1141 atttaaacta ccagctgcat ttgagataaa taattttttt atccctaata atttcatggt
     1201 gcgaacaggg aaaactactt cttccatagt atatccttcg taataatgga aacgcccttg
     1261 catggccacc acttttttat cgccaatgct gccaaatatt aatttaccgc tatgactttc
     1321 aacagtagaa acctgaaaat gaggtattaa agaataattt atttcatgtg caattttgat
     1381 atgtgatacc agattgccca aaccggtgcc taaaataatg ccgtattctg gttcaaagtt
     1441 ggtgcgttgt ttaatataat gtaccgcttc gtttaatctg tccattagtc gttccatagt
     1501 attactcatt tatattacaa agatagtatt tgaaaaaatg gttttagttg tttgcctgtt
     1561 ctgcattggt atcaggatat ttatcgataa aggttgttat cagattccag aatttttctt
     1621 cttcccctaa aaagtgtaca tctactggta acacaaaaac ggaaaagccc atttgtaaca
     1681 ggatatcctt ttgttccgat aatcgcatcg catctttttc ggtggtgata atggttttgg
     1741 tatcggggaa actacctgca acacgcaaaa aatctttctc cctaaaataa tggtggtcgc
     1801 taaaagctgc gatatgaata gttgaaaatt tgccggacaa atacgatttc aattgtttgt
     1861 tgcctgctat gcctgtcatt aatattacag attgctgaaa aggtatctgc actattggct
     1921 gtt
//
LOCUS       DAORKW010000658         1921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3878_length_1921_cov_4.214362, whole genome shotgun sequence.
ACCESSION   DAORKW010000658 DAORKW010000000
VERSION     DAORKW010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3878_length_1921_cov_4.214362"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLB46_16670"
     CDS             complement(<1..526)
                     /locus_tag="PLB46_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRF78214.1"
                     /translation="MKAIIPVAGVGTRLRPHTYSQPKPLIPVAGKPILGFIIDRLVKA
                     GCTEFVFVIGYLGEKIEAFVRETYPDLSTRFVIQNKREGLGHAIYLTKDYVSKGEDIF
                     IVLGDTIFEGDIESVIQSTDSALGLKRVDDPRDFGVAEVDEAFNIIRVIEKPSIPKSN
                     LALVGLYKIKEAKIL"
     gene            726..>1921
                     /locus_tag="PLB46_16675"
     CDS             726..>1921
                     /locus_tag="PLB46_16675"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRF78215.1"
                     /translation="MKKRLLLLLTAGFVTYTCAFSQVVINEYSAANFSDIADNYGEYE
                     DWIELYNAGATAVALAGYHLSDNADDPTKYVIPAGVNIPAGGFVRFWCSNRNTVVGTN
                     YHTNFKLTQTTGSEEIVFADAAGTVLDELEIDKVNQTNHSWGRSPNGGADWRIYTDPT
                     PNASNSTPTKIAYAKKPDMEPNSGNYAGSVTVTITVEEPNMVIRYTTDGAFPTAAATA
                     YTGPITIDETTVLRTVAYPVSTDTLLSLCSTNTYFIDETTEMPIISVAGEQIDNLLNA
                     TGWGIQPIGSFEIFDKDFNLIDEATGSFNEHGNDSWAYDQRGFDYIVHDQFGYDNDID
                     HEFFADVTDRTGYQRMISKAGANDNYPSSDGGAHVRDAFVHHIAQLDDLELDERSSFF
                     NIDPKRP"
BASE COUNT          593 a          388 c          358 g          582 t
ORIGIN      
        1 taagaatctt tgcttcttta attttatata aacctaccaa cgctaaattt gattttggga
       61 tggaaggttt ttcaatcacg cggataatat taaatgcttc atcaacttca gcaaccccaa
      121 aatcgcgagg gtcatcaaca cgttttaatc ccaaagcaga atcagtactt tgtattaccg
      181 attcgatatc tccttcaaaa atggtatcac ctaaaacaat aaaaatatct tcgccttttg
      241 atacataatc tttagttaga taaatggcat gtcctaaacc ttctcgttta ttttgaatca
      301 caaaacgcgt agataaatca ggataagttt cacgcacaaa ggcttcaatt ttttcaccca
      361 aataaccaat cacaaataca aactcggtgc atcctgcttt caccagcctg tcgatgataa
      421 aacctaaaat gggtttaccc gcaaccggta taagcggctt gggctgcgag tatgtatgtg
      481 gacgaagacg ggttcctacc ccggcaactg gtattatagc cttcatgctg ttgaattagg
      541 gcgctaagtt aacccatcct gtaaaatatg cagaactaca ttttttgggg ttttgtgaca
      601 aaaactaaac tttttagcca tttgattgta attttcgtgt cagtttcaac ctaataatcg
      661 aattcgttcg taaatttata ggttcaaatc attggcactc aaatcatcaa gtaattgaaa
      721 attatatgaa aaaacgttta ctcctccttt taaccgcagg atttgtaacc tacacctgtg
      781 cattttctca ggtagttatc aatgaatatt ctgctgcaaa ttttagcgat atcgccgata
      841 attacggcga atacgaagac tggattgagc tttataatgc aggcgctact gcagttgcac
      901 ttgccggtta tcacttaagt gataatgctg acgaccctac taaatatgta attccggccg
      961 gggtaaacat tcccgcaggt ggttttgtac gtttttggtg ttcaaaccgt aacactgtag
     1021 ttggaactaa ttaccacacc aattttaagc tgacacaaac tacaggttca gaagaaattg
     1081 tttttgccga tgctgcagga actgttttag acgaattgga aattgacaaa gtaaatcaaa
     1141 ctaaccatag ctggggacgc tcaccaaatg gtggtgctga ctggagaatt tataccgacc
     1201 caacacctaa tgcatctaac agtacaccaa ccaaaattgc gtatgctaaa aaaccggata
     1261 tggaaccaaa ttccggaaat tatgccggtt cggtaactgt tactatcact gttgaagaac
     1321 ctaatatggt aattcgatat acgacagatg gcgcatttcc taccgctgct gcaacagctt
     1381 acacaggccc gatcactatt gatgaaacta cagttttgag aactgttgca tatcctgttt
     1441 ctacagatac attattatca ctttgctcta ctaatactta tttcattgat gaaacaaccg
     1501 aaatgccgat aatttctgtg gcaggggaac aaatagataa cttattaaat gctaccggtt
     1561 ggggaatcca accaattggc tcatttgaaa tttttgacaa agattttaat ttgattgatg
     1621 aagctactgg ttcatttaat gaacacggga atgactcatg ggcttacgac caaagaggtt
     1681 tcgattatat tgtgcacgat cagtttggtt atgacaacga tatcgaccac gaattttttg
     1741 ccgatgtaac agaccgcaca ggttatcagc gtatgatttc taaagcaggt gcaaacgata
     1801 actatccatc atctgatggt ggtgcgcacg tgcgtgatgc ttttgttcac cacattgctc
     1861 aattagacga tttggaattg gatgagcgtt catctttctt taatatagat ccgaagagac
     1921 c
//
LOCUS       DAORKW010000659         1917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3894_length_1917_cov_2.880773, whole genome shotgun sequence.
ACCESSION   DAORKW010000659 DAORKW010000000
VERSION     DAORKW010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3894_length_1917_cov_2.880773"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="PLB46_16680"
     CDS             <1..316
                     /locus_tag="PLB46_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78216.1"
                     /translation="IFFAITRGIPSLILFFIVVKLLSKTSWSLRYQLWVMALFGAVCC
                     AANVIGFVYRKKMDEIFIHDVLLISAYSLTVILGVYLFRKTLQNSGALTEQKEDAPLP
                     IP"
     gene            339..1007
                     /locus_tag="PLB46_16685"
     CDS             339..1007
                     /locus_tag="PLB46_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78217.1"
                     /translation="MTTLVNLLQHKGNFINGITTNDNLKRRITDLLITSTIAFAAYGF
                     IIGLQQGILQAMAGMVKLPMLYLLTLMICLPTYFIFDSFLGSKSNILQTVALALTGIT
                     ITSILLVGFAPVTLFFLLTMNNYVAFKLLNVLFFTISGFIGARQLYVLLLGELEEGNG
                     KNFVRRKKLLRLWLLLYAFVGTQMAWTLRPFFGVQEGKFIFFQEIRGNFYTNLLHNLI
                     EIFK"
     gene            1062..1421
                     /locus_tag="PLB46_16690"
     CDS             1062..1421
                     /locus_tag="PLB46_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78218.1"
                     /translation="MKSTRTKYRLLWNFLVLPIGCTISLIPFVIFEIRVNHTITFTEL
                     ASQTIAETPSLLEPVKLVFPVIFAIIGIIISVIYFNLRIVCDVWTNFTNTHFRVINII
                     LIIIADLAILLFVSHIT"
     gene            1601..>1917
                     /locus_tag="PLB46_16695"
     CDS             1601..>1917
                     /locus_tag="PLB46_16695"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRF78219.1"
                     /translation="MPLLLDLSTPFTEPVIIFTLVLCIILISPILLNKLRIPAIVVMI
                     VAGVTIGPHGFHLLERDGAIVLFGTVGLLYIMFLAGLEIEMNGFKKNAKSSVVFGLLT
                     FAVP"
BASE COUNT          551 a          342 c          320 g          704 t
ORIGIN      
        1 gatatttttc gctattacca gaggtattcc ttccttgata ttatttttta ttgtcgttaa
       61 actactatcc aaaacatcat ggtcactccg atatcaactt tgggtaatgg cgttgtttgg
      121 cgcggtatgt tgtgctgcca atgttatcgg atttgtttat cgcaaaaaaa tggacgagat
      181 ttttatacac gatgtgctat taatttctgc gtatagttta accgttatcc tgggtgttta
      241 tttattccgt aaaactttac aaaattcggg agcattaacc gaacaaaagg aggatgcccc
      301 attacctatt ccataacttc atctattaaa ttacaactat gaccacactc gtaaatttat
      361 tacaacacaa aggcaatttt attaatggta taactactaa cgacaacctg aaacgccgca
      421 ttaccgattt attaataacc agcacaatag catttgctgc atacggattt ataatcggct
      481 tacaacaagg gatattacaa gcaatggccg gcatggtgaa attaccaatg ttatatttat
      541 taacgttaat gatttgtctc ccaacctatt ttattttcga cagtttttta ggttctaaat
      601 ctaatatttt acaaacggta gccctcgcat taacaggaat taccattacc tctattttat
      661 tagtagggtt tgctccggta actttatttt ttctgttaac catgaataat tatgtggcgt
      721 ttaaattgtt gaacgtatta ttttttacca tatcaggttt tataggtgct cgccaattgt
      781 atgtattgtt gttgggcgaa ttagaggaag gaaacgggaa aaattttgta cgtagaaaaa
      841 aactattgcg cttatggtta ttattatatg cctttgtagg cacacaaatg gcctggacct
      901 tacgtccatt ttttggtgtg caggaaggga aatttatttt ttttcaggaa atacggggta
      961 atttttatac caacttattg cataatctta ttgaaatttt caagtaggtt tttgtaaatt
     1021 gcggttctat aatccactac aattggatta taaatgcaat attgaaatca acgcgtacaa
     1081 aatatcgttt gttgtggaat tttcttgtgc ttccaattgg ctgtactatt tcattaatcc
     1141 cattcgttat ttttgaaata cgagtaaacc atactatcac gttcaccgag ttggcaagtc
     1201 aaactattgc agaaacacca agtttattag aacctgtaaa gcttgttttt cctgtaattt
     1261 ttgccattat tggtattatt atttcagtaa tttattttaa tctgcgaatt gtttgcgatg
     1321 tatggacaaa ttttaccaat actcatttta gagtaattaa cattatttta ataataattg
     1381 cagatttggc aatcctattg tttgtctcgc atatcacgta atgtatattt ttaggtaaaa
     1441 cattatacct tttacctatc taaataacac ttatttttaa atttcagatt aaatactcac
     1501 tgattacata atataattaa ccatccccta ccctgctttt gacctttagc acctaccatc
     1561 gtaattactt ccgtatcttt gcggcaaaat ggaaagactg atgccgctcc tgcttgattt
     1621 gagcactcct tttacggagc cggttatcat atttaccctg gtgttatgca tcatcctcat
     1681 ttcacctata ttgttaaaca agctacgcat tcccgccatt gtggtaatga ttgtagccgg
     1741 tgtaaccatt ggtccgcatg gctttcattt acttgaacgc gatggcgcta ttgttttgtt
     1801 tggtacagta ggtttactat atatcatgtt tttagccggt ctcgaaattg agatgaatgg
     1861 cttcaaaaaa aacgcaaaat ctagtgtggt tttcgggcta ctcacctttg cggtccc
//
LOCUS       DAORKW010000660         1915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3901_length_1915_cov_4.137097, whole genome shotgun sequence.
ACCESSION   DAORKW010000660 DAORKW010000000
VERSION     DAORKW010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3901_length_1915_cov_4.137097"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1423)
                     /locus_tag="PLB46_16700"
     CDS             complement(23..1423)
                     /locus_tag="PLB46_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRF78220.1"
                     /translation="MNLKQFTIKSQEVIQQAQQLAMQLDHQQITNEHIMKSLLSGDDE
                     VIGYLLKKLNVNVLYVQQKVDELVDNLPKMPGKGGEFISRDANLTIAKSQTFLKEFGD
                     EFVSVEHLLLGILTVNDAVGKLLKSQGVTEKDLKAAIKELRKGSTMNSQSGENQVNAL
                     NKYAINLNDRAKSGKLDPVIGRDEEIRRVLHILSRRTKNNPILVGDPGVGKTAIAEGI
                     AHRIVNSDVPENLKSKIIYSLDMGALIAGAKYKGEFEERLKAVIKDVIQSEGQIILFI
                     DEIHTLVGAGGGGEGAMDAANILKPALARGELRAIGATTLNEYQKYFEKDKALERRFQ
                     KVMVDEPSWEDAISILRGLKERYENHHKVRIKDEAIIASVELSSRYIADRKLPDKAID
                     LIDEAAAKMRLEMDSVPEALDEIERRIRQLEIEREAIKREKDEKKVEELSKDIAELSE
                     ERNAIKAKWQQEKGLD"
     gene            1724..>1915
                     /locus_tag="PLB46_16705"
     CDS             1724..>1915
                     /locus_tag="PLB46_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78221.1"
                     /translation="MKQTTSILLALAICLMLTVDAAGQTPNCANDSSGLIPLNDLGSG
                     YYMGKQGGLFPNGANAEDPT"
BASE COUNT          539 a          418 c          299 g          659 t
ORIGIN      
        1 ctggacgatc tggcctgtag tatcaatcaa gccctttttc ctgttgccat tttgctttta
       61 tggcatttct ttcctccgac aattcagcaa tatctttact cagctcctct actttctttt
      121 catccttttc tcttttaatc gcttcacgtt caatttccag ctgacgaata cggcgttcaa
      181 tttcatcgag tgcttcagga accgaatcca tttctaaacg catttttgca gcagcttcat
      241 caattaaatc gatagcttta tccggtaatt tcctatctgc aatataacgt gaacttaatt
      301 ctacactggc aataatagct tcatctttaa tgcgcacttt atggtgattt tcgtaacgct
      361 cttttaatcc gcgtaaaatc gaaatcgcat cttcccaact tggttcgtcc accattactt
      421 tttggaagcg acgttccagt gctttatctt tttcaaaata tttttgatat tcatttaagg
      481 tggttgcacc aatagcacgt aattcaccgc gggctaatgc aggttttaaa atattagctg
      541 catccatagc accttcgcca ccaccgccgg caccaactaa agtgtggatt tcatcaataa
      601 ataaaatgat ttgcccttca ctttgaatga cgtctttgat aactgctttc aatcgttctt
      661 caaattcgcc tttgtattta gcgccggcaa ttaatgcacc catatcgagt gaataaataa
      721 ttttcgattt caaattttca ggcacatcac tatttacaat tctgtgcgca ataccttcag
      781 caattgctgt ttttcccaca ccggggtcgc cgactaaaat ggggttattt tttgtgcgtc
      841 gcgaaagaat atgtaatacg cgtcgaattt cttcatcacg accaataaca gggtctaatt
      901 ttccactttt tgctctgtcg tttaaattaa ttgcatattt atttaatgca ttcacctgat
      961 tttctccgct ttgagaattc atggtggaac ctttacgcaa ttctttaata gcagccttta
     1021 aatctttttc ggtaacacct tgcgatttta ataatttacc aacggcatcg ttaaccgtta
     1081 aaattcctaa taacaagtgt tctacagaaa caaattcgtc gccaaattct tttaaaaatg
     1141 tttggctttt tgcaatggtt aaattggcat cacgagaaat aaattctccc cctttaccgg
     1201 gcattttagg caggttatct actaactcat caactttttg ctgcacatat aacacattca
     1261 cattgagctt tttcaataag tacccaatca cttcatcatc gccacttaaa agcgatttca
     1321 taatatgttc gttggttatt tgttggtgat ccaattgcat ggctaattgc tgagcctgct
     1381 gaataacttc ctgcgatttg atggtaaatt gtttcagatt catacgtttt tgttttttaa
     1441 aagagataat ccaaggtaac attccttact tatacactta atctattcac cttgtttact
     1501 taaacacaaa ctaagccgtc aaaaagcata cttgattgaa aatcaacgat ttttacgcca
     1561 ttttgaccta aataacttgt cgaatgcgac aaattgactg tgcaattcgt ctccataagc
     1621 ctttatgtct aacgaatatt ggacctgtat taacatctac atatgcttaa tataaaatac
     1681 atatgattag atttgcatgg acttagtttt gaacacacac acaatgaaac aaacaacaag
     1741 tattttgttg gcgttagcta tatgcttaat gctgactgtt gatgctgcag gacaaacccc
     1801 aaactgcgca aacgattctt cgggactcat cccgctgaat gatttaggaa gcggctatta
     1861 tatgggcaaa caaggaggtt tattcccaaa tggagccaat gccgaagacc ctact
//
LOCUS       DAORKW010000661         1911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3915_length_1911_cov_5.614224, whole genome shotgun sequence.
ACCESSION   DAORKW010000661 DAORKW010000000
VERSION     DAORKW010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3915_length_1911_cov_5.614224"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PLB46_16710"
     CDS             complement(<1..1488)
                     /locus_tag="PLB46_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78222.1"
                     /translation="MKKVFSILSAVLLTAAAFQSFGQDQQWVNYSNFTAVTDVEPVGE
                     HLWITAKGGVLDLNTTTNTKTYYKQGDAGLPSSSVEQIAYDASSGTIWVGTYDAGVVE
                     WDGENWLTYDYPMSFLMYRMKMDGFGNIWLQTDAGLYKFDREDFSYTFINSVGGAGWS
                     FEAWDFDITPDNHVLIFTGEDCLVIDAVTNMAIDSFPNTESPVVLGCSPSTVRIYGAD
                     ADTYLINNSGSLEFQHKDGTYESANTGIPEFAFINNITRGTDNNLYALVNSMEIYQLV
                     GAEWELKNTIADVYPDKLMFTDGTTHHLNTTGYMVPPTVVELSDMGQTIIETKKYNFN
                     SNAISGITVDDAGDIFMSSATTIYKYNAGINDWDMVTAVPTIYGSMIDLRYKNGNFYT
                     IDNGNLIEYYNGTEWQHIPIAAGYESIYIFDFDITDDGTIYFTNDEGLFKYKDGVTIN
                     LIETGPVNKWFLSVKYDVVNNLVWLGRTTAIIKYDFISEELIDDND"
     gene            1635..>1911
                     /locus_tag="PLB46_16715"
     CDS             1635..>1911
                     /locus_tag="PLB46_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78223.1"
                     /translation="MKNPSNDLFQLIQSLSKSEKRYFKLFAIRHFDKDNQYLKLFEAI
                     ETQLTYQETLLVPMFSEGKSAAHFAVLKKQLYESLLEALHRFDEFDDA"
BASE COUNT          625 a          382 c          305 g          599 t
ORIGIN      
        1 atcattatca tcaataagtt cttcactgat gaaatcatat tttataattg cggtagttct
       61 gcccaaccat accagattgt ttaccacatc gtattttaca cttaaaaacc atttgttcac
      121 cggaccggtt tcaatcaggt taatggtaac accatcttta tatttaaata aaccttcgtc
      181 gttagtaaag taaatagtac catcatcagt aatatcaaaa tcgaaaatat aaattgattc
      241 gtatcctgca gcaattggaa tatgttgcca ttcagttcca ttgtagtatt caatcaaatt
      301 accattatct atagtataaa aattaccatt tttatatctc aggtcaatca tagaaccata
      361 aatagtaggc actgcagtta ccatatccca atcgttaata cctgcattat atttataaat
      421 ggtagttgct gacgacataa atatatcccc tgcatcatct accgtaatac ctgaaatagc
      481 atttgaattg aaattatatt tttttgtttc aataatggtt tgtcccatat ccgacaattc
      541 aactacagtt ggaggtacca tatatcctgt ggtatttaaa tgatgtgtag tgccatcagt
      601 aaacataagt ttatcaggat acacatctgc tatcgtattt tttaattccc attcagcgcc
      661 aactaactgg tatatttcca ttgaatttac caatgcatat aaattattat cagtacctct
      721 ggtgatatta ttaataaatg caaattcagg aatacccgtg ttggccgatt cataagtacc
      781 gtctttatgt tgaaattcga gactgccact attgttaatt aaataagtat cagcatctgc
      841 gccataaatt ctcactgtag aaggagaaca tcctaataca acaggtgact cagtatttgg
      901 aaatgaatcg atagccatat tggttacggc atcaattacg aggcagtctt cacctgtaaa
      961 tattaataca tgattatcag gtgtaatatc aaaatcccaa gcttcaaacg accagcctgc
     1021 accaccaact gagttgatga atgtataact aaaatcttca cggtcgaatt tgtataaacc
     1081 tgcatcagtc tgcaaccaga tattaccaaa accatccatt ttcatgcgat acattaaaaa
     1141 tgacatcggg taatcataag tcagccaatt ttcaccatcc cattccacaa caccggcgtc
     1201 ataagtgccc acccaaatgg tacctgaaga ggcatcatag gcgatttgct caaccgaaga
     1261 agagggcaaa ccggcatcac cttgtttgta ataagttttg gtattggtag tggtatttaa
     1321 atccagcact ccaccctttg cggtaatcca aaggtgctcg ccaaccggct caacgtcggt
     1381 aactgctgta aagttgctgt aattaaccca ttgctgatcc tggccgaagc tctggaaagc
     1441 agcagcagtg aggagcactg ccgaaaggat actgaatact tttttcatat aatttcaatt
     1501 ttcaaggaca aatttcatcc tctttatact ataaaagaaa tcaaattaat gtcataaatt
     1561 agatgtctga atgaatttta gctgatttat atcaaacgat aacagcattt tcattacttt
     1621 tacattagaa gtctatgaaa aacccttcca acgatttatt ccagctgata cagtcgctct
     1681 ccaaaagtga aaaacggtat tttaagctgt ttgccatcag acatttcgat aaagataacc
     1741 aatatttgaa gctgtttgag gctattgaaa cacaattaac atatcaggaa accttgcttg
     1801 tcccaatgtt ttcggaaggt aaatcggctg cccattttgc agtccttaaa aaacaattgt
     1861 atgaatcgct gctggaagcc ttacataggt ttgatgaatt tgatgatgct g
//
LOCUS       DAORKW010000662         1898 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3963_length_1898_cov_5.204558, whole genome shotgun sequence.
ACCESSION   DAORKW010000662 DAORKW010000000
VERSION     DAORKW010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3963_length_1898_cov_5.204558"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PLB46_16720"
     CDS             complement(<1..811)
                     /locus_tag="PLB46_16720"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRF78224.1"
                     /translation="MQKIKSFYQSARGKFIITITGISSVFVLTSAVTSADLFEIGKNI
                     DIFTSVYKELNTYYVDDVDPNKLMRTGIDAMLESLDPYTNYISEAELEGYKFQITGNY
                     GGIGASIRTIDGQSTVVECFEGFAAEKSGLIPGDIILEIDGKKLADVNQDGISDLLRG
                     SPGTTLTLKVNRPLDNKTFDLAIVREDIDVHNVPYYGFVEDGIGYIILNQFTEDAGLN
                     VGNAVKELKKLNPAMSGIILDLRGNPGGLLREAVNVSNVFIDKGEEVCSTLG"
     gene            complement(835..1065)
                     /gene="yidD"
                     /locus_tag="PLB46_16725"
     CDS             complement(835..1065)
                     /gene="yidD"
                     /locus_tag="PLB46_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRF78225.1"
                     /translation="MKYISKFFGWILILPVKIYQLVLSPILPNSCRYTPTCSHYMVTA
                     IQKHGPIKGLWLGIKRISRCHPWGGHGHDPVP"
     gene            complement(1116..1499)
                     /locus_tag="PLB46_16730"
     CDS             complement(1116..1499)
                     /locus_tag="PLB46_16730"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRF78226.1"
                     /translation="MFTRATLSKSERLTKEQLIASLFEAGQSFLSPPFVVHYQITTLP
                     DKVPAQFLASVSKRKFAKATDRNTLKRRMKEAYRLNKTPLYTHLLNQNKQLAVALIYN
                     EKKITSCNDIESKIKVALSELISRI"
     gene            complement(1534..1692)
                     /gene="rpmH"
                     /locus_tag="PLB46_16735"
     CDS             complement(1534..1692)
                     /gene="rpmH"
                     /locus_tag="PLB46_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRF78227.1"
                     /translation="MKRTFQPSRKKRRNKHGFRERMATKDGRAIIARRRRKGRKKLTV
                     SDERNIGK"
BASE COUNT          576 a          354 c          307 g          661 t
ORIGIN      
        1 ttccaagtgt gctgcatact tcttctcctt tatcaataaa tacatttgaa acattaaccg
       61 cttcacgcaa caaaccaccc ggattgccgc gtaagtcaag tataatgcca ctcatagccg
      121 gattaagttt ctttaattct tttacggcat taccaacatt aagacctgca tcttcagtaa
      181 attgatttaa tataatatat ccgataccat cttcaacaaa accgtaataa ggtacattgt
      241 gcacatcaat atcttctctt acaatagcaa gatcaaatgt tttattatct aacggacgat
      301 taacttttaa tgttaatgtt gttcccggtg acccacgtaa taaatcacta ataccatctt
      361 gatttacatc ggctaatttt tttccgtcta tttccaaaat gatatctccg ggaataagtc
      421 ctgatttttc agcagcaaat ccttcaaaac attctacaac tgtcgactgc ccgtcaattg
      481 ttcttatgct tgctcctata cctccgtaat ttcctgttat ttgaaattta tacccttcta
      541 attctgcttc agatatataa ttagtataag ggtccaaact ttccagcatc gcatcaatac
      601 ctgtgcgcat taatttatta ggatctacat catctacata ataggtgttt aattccttat
      661 atacagaagt aaatatgtct atgttttttc ctatttcaaa taaatcggca ctggttactg
      721 cagaggttaa aacaaatacg gatgaaatac ctgtaatggt aataataaac ttacctcttg
      781 ctgattgata aaatgattta attttttgca tacaataaaa acaatttaaa ttaattaagg
      841 aacaggatca tgaccatggc caccccacgg atgacaacga cttattcttt taattccaag
      901 ccataaacct ttaattggtc catgtttttg aatagcggtt accatataat gactacaggt
      961 tggtgtatac cggcaagagt ttggaagaat gggtgataat actaattgat aaattttaac
     1021 aggtaaaatc aaaatccaac caaaaaactt gctgatgtat ttcattgtgt ttttaaccta
     1081 tatgactttc atctaacgat aatattgtgc ttttgttata tccttgaaat tagttcggat
     1141 aaagctactt tgattttaga ttcaatgtcg ttacaagatg ttattttttt ctcattgtat
     1201 attaaagcta cagccagttg tttgttttgg ttcagcaagt gcgtatacaa aggggtttta
     1261 tttaagcgat aagcctcttt cattctgcgt ttcaacgtat ttctgtccgt tgctttcgca
     1321 aatttacgtt tcgaaacaga ggctaaaaac tgggctggaa ccttgtccgg tagagtggta
     1381 atttgataat gaaccacgaa cggtggggat aaaaaacttt gccccgcttc gaaaagcgag
     1441 gcaataagtt gttctttggt aagtcgctcg gacttactaa gtgtagctcg tgtaaacata
     1501 cgaccagcgc tgttttcaga gcctgaagtt ggtttattta ccgatatttc tttcgtcaga
     1561 aacagtcaat tttttacgtc ctttacggcg acgacgtgca ataatagcac gaccatcttt
     1621 agttgccata cgctcacgga atccgtgctt atttcttctt ttctttcttg aaggttgaaa
     1681 cgtccttttc atcgcttact attttgggaa tgcaaagata ggatttactt tgcgaaattc
     1741 acaatctgtc aacaaataat gttttaagta tttctttttg atgactaata attgatttag
     1801 gaggaccatt tttatgtata aaccgtcaag ttttttctaa attgaacgaa gtgacaaata
     1861 aaaataaccc gcaaatttgt tgaattttgc gggttaag
//
LOCUS       DAORKW010000663         1895 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3968_length_1895_cov_5.099457, whole genome shotgun sequence.
ACCESSION   DAORKW010000663 DAORKW010000000
VERSION     DAORKW010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3968_length_1895_cov_5.099457"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PLB46_16740"
     CDS             complement(<1..530)
                     /locus_tag="PLB46_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUB domain-containing protein"
                     /protein_id="HRF78228.1"
                     /translation="MKNNDMKMQAQNKSMLLVFLIGILSLTSAFSQTYTHPTAGIDGT
                     YVGACMVNTSTGDYYDNGGAASNYSNNIGTSFAGSKNGIYRVFCPSIAYNCVSVTFNT
                     FDTEPHATCNLDFLTIGNGATQNSTLLSIPGVTIGSGRICGSPTVPFTATSTDGSGCL
                     SFRFFSNNSNTYAGWSP"
     gene            complement(520..777)
                     /locus_tag="PLB46_16745"
     CDS             complement(520..777)
                     /locus_tag="PLB46_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78229.1"
                     /translation="MSELKVLPSNLERYYIDYEIVGLPNPTASDVANLDLNQYEDLRA
                     QTTSVLIEDAATGYVLLLYSINESIKNWQGLNHNSKDHIEK"
     gene            complement(1062..1628)
                     /locus_tag="PLB46_16750"
     CDS             complement(1062..1628)
                     /locus_tag="PLB46_16750"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRF78230.1"
                     /translation="MIKNKVKPAAGHLLLSEPFMPDPNFKRTVIMLCEHLEDGGTVGF
                     ILNKSLNVKVCDALVDFDAVTNDLFYGGPVAKDTLHYLHRYGDIIEDSLHIADDVYWG
                     GNFEQIGELLRDGTLDPKNIKFFLGYSGWSPGQLDDELTDNAWIVTKAKSDYIFDSAN
                     KELWKKVLKDLGGEYTQIVNYPEDPSLN"
BASE COUNT          629 a          380 c          315 g          571 t
ORIGIN      
        1 ggggaccaac ctgcgtaggt attagaattg tttgaaaaaa aacggaaaga taaacagcca
       61 ctaccgtcag tactagttgc ggtaaatggc actgttggtg aaccgcaaat tcgaccggaa
      121 ccaattgtta cacctggtat ggaaaggaga gtggaatttt gtgttgcacc attaccaatg
      181 gttaaaaaat ccagattgca agttgcatgt ggttcggtat caaatgtatt aaaagttaca
      241 gaaacacaat tgtaagctat acttggacaa aacaccctgt aaataccatt tttactgcca
      301 gcaaaacttg tcccaatatt attggaataa ttcgatgctg caccgccatt atcataataa
      361 tcaccggtag atgtatttac catgcaagcg ccaacgtaag ttccatcaat tcctgctgta
      421 gggtgagtat aagtttgaga aaacgcagac gtcaaactca gtattcctat taggaacacc
      481 agtaacatac ttttattttg agcctgcatt ttcatatcgt tatttttcaa tatggtcttt
      541 tgaattgtga ttcaatccct gccaattttt aatactttca tttattgaat acagtaacaa
      601 tacataaccg gttgcagcat cctctattaa tacggaagta gtttgagcgc gtaaatcttc
      661 atattgattc aaatccaaat tagctacatc cgatgcggtc ggatttggca aaccaacaat
      721 ttcataatcg atgtaataac gctctaaatt ggatggaagc accttaagtt cagacacact
      781 ttgtgcagag cagtagctat agcatactat tcctagtaca agtagaattg tatttcttaa
      841 acaatgcatg tgtgacaatt ggtaaaccaa aaataacaaa tagtttacat ataagaagac
      901 ctcaatctga aaaaccttat ttttagcaca gttttaacac taaaagtcct gctgaatgaa
      961 ggttacaggc cttgataaca gttaacggtt tttcgcaaat tagtttcatt taccggctag
     1021 tccttgccaa aagaggaaaa cgacgttgac ttaaagctat tttagtttaa actcggatct
     1081 tccgggtaat tcacaatttg agtatactcg ccccctaaat cctttaatac cttcttccaa
     1141 agttctttgt tggcagaatc aaatatgtaa tcactttttg cttttgtaac aatccaggca
     1201 ttatcggtaa gttcatcatc aagttgcccc gggctccaac cactatatcc aagaaaaaat
     1261 ttgatgtttt ttgggtctaa agtaccatct cgcaacaatt cgcctatttg ctcaaaatta
     1321 cctccccaat acacatcatc cgcaatatgt aaactgtctt caatgatgtc gccatatcta
     1381 tgcaaataat gtaaggtatc ttttgcaacc ggaccaccat aaaataaatc gttagtaacg
     1441 gcatcaaaat caaccagtgc atcacaaacc ttcacattaa gcgatttgtt taaaataaag
     1501 ccaaccgtgc cgccatcttc caaatgttcg cataacataa taacagtccg cttaaaattc
     1561 ggatcaggca taaagggctc gctcaataaa aggtggcctg cagcaggttt taccttattc
     1621 ttaatcataa cacgcttatt tatatggtta ggcaggtcaa atatccgtat tatgcggtat
     1681 aaaccaaatt tttttcaaag cagttcgaaa gctatcaacc tgccatccac atttacttag
     1741 tatagtgatt acttaaaatt aacatcccaa aaaccaaata gttgttaaat ttgatgcaac
     1801 ttaatatagc aaaagagaaa aaatgaaaaa attgtacttt gtattcgggg ctttgttatt
     1861 agctatgaca ggcaacgcgc agctttcaat tccaa
//
LOCUS       DAORKW010000664         1892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3986_length_1892_cov_4.360370, whole genome shotgun sequence.
ACCESSION   DAORKW010000664 DAORKW010000000
VERSION     DAORKW010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3986_length_1892_cov_4.360370"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLB46_16755"
     CDS             <1..290
                     /locus_tag="PLB46_16755"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HRF78231.1"
                     /translation="EYKKELEEKLRSTFEDKLKTFTRSQGEVLIDIIERNTNESMYNI
                     LKEHKSGSSAFWWNSLSKAYGYDLKDKYRPDNNPTLEKIIQDYETKHKKKE"
     gene            complement(297..1586)
                     /locus_tag="PLB46_16760"
     CDS             complement(297..1586)
                     /locus_tag="PLB46_16760"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRF78232.1"
                     /translation="MKSLINFKFIASGVLLLLQFNLLKAQAISKSALFIGNSYVYTND
                     LPAQIKNIAISMGDELITDSNTPGGYTFELHSTNATTLSKIALGTWDYVILQEQSQIP
                     SFPIGQVETICFPYATILDDLIHEANSCAETVFFMTWGRKEGDAGNCPTWPPVCTYEG
                     MDSLLNLRYRMMAEDNDALLSPVGQVWYFIRDLYPEIELYNPDFSHPSVNGTYAAALT
                     FYTILFQKNPLDITYNGSVTPDNAMHMREVVKSVVYDSLAVWHVGEYYPQAAFSFENL
                     GDAVYQFTNLSTNAESYVWDFGDGTTSTEINPIHTYTAAGNYPVKLVANKCANNDSTT
                     SELVVENVGIIQTHYNDGFTIYPNPVQNNSLIFSAEEQTFSTYCIYNNEGQMIMSGDL
                     FFLENVITTENLPDGFYLFTALKATGEMVSKPFTVSR"
BASE COUNT          616 a          350 c          353 g          573 t
ORIGIN      
        1 aagaatataa aaaggaatta gaagaaaaat tgcgcagtac atttgaagac aaactaaaaa
       61 cattcaccag gtcgcagggc gaagttttaa tagacattat tgagcgcaat accaacgaat
      121 cgatgtataa tatcttgaaa gaacataaga gcggcagttc cgctttttgg tggaacagtt
      181 tgagtaaagc ttatggatac gatttaaaag ataaataccg gcctgataat aatccaacct
      241 tggaaaaaat aatccaggac tacgaaacta aacataaaaa aaaggaataa agcttattat
      301 ctgctcacgg taaaaggctt tgaaaccatt tctccagttg ctttaagggc agtaaatagg
      361 taaaagccat caggcaaatt ttcagtggta attacatttt ccaaaaagaa taaatcgcca
      421 ctcattatca tttgtccttc gttattataa atacaatatg ttgaaaatgt ttgttcctca
      481 gctgaaaaaa ttaaactatt attttgaacc ggatttggat aaatggtaaa accatcgtta
      541 taatgggttt gtattatccc aacattttct actactaatt cagatgttgt tgaatcgtta
      601 tttgcacatt tatttgctac caattttacc ggataatttc cggcagcagt ataagtatgt
      661 attggattaa tttctgttga agttgttcca tcaccaaaat cccaaacata actttcggca
      721 tttgtagaca gattggtaaa ttgataaacc gcatcaccta aattttcaaa actaaatgct
      781 gcttgcggat agtattcccc aacatgccaa acagccaaac tatcatatac tacagattta
      841 accacttcac gcatatgcat ggcattatca ggagttacac ttccattata cgtaatatcc
      901 aatggatttt tttgaaataa aatagtgtaa aaagtcagtg ccgctgcata agtgccattt
      961 accgaaggat gactaaagtc cggattgtat aactcaattt cgggatacag gtccctgata
     1021 aaataccaaa cctgtcccac aggtgataaa agagcatcat tatcctctgc catcatccga
     1081 tatcgcaggt tgagcaaact atccatgcct tcgtaggtgc atacaggagg ccaggttggg
     1141 cagtttcctg catcgccttc tttcctaccc catgtcataa aaaatactgt ttcagcgcag
     1201 ctattggctt cgtgaattaa atcatcgagt atggtggcat aaggaaaaca aatagtttca
     1261 acttgcccaa ttggaaatga tggtatttgg ctttgctctt gtaggatcac ataatcccag
     1321 gtccctaagg ctattttgct aagtgtggtg gcattggtgc tgtgtaactc gaatgtataa
     1381 cctccgggtg tattactgtc cgttatgagc tcatcgccca tggatatggc tatattttta
     1441 atttgtgcag gtaagtcatt ggtatagaca taactattgc caataaataa agccgattta
     1501 gaaattgcct gtgctttgag cagattgaat tgcagcagta acaggacacc tgatgcaata
     1561 aacttaaagt taatcagaga tttcatttcg aaataaaaat aacaaataag ggtcggttcg
     1621 tgtttttagg ttatggaaga tgacaatttt acgacttaat taatatcgaa cattccattt
     1681 ttgaatccaa ccacaataga tttcctaccg ctgcaggggt aaaaaaaagc tggtactaaa
     1741 taagcaccag ccttaaaatg ttttgcttta attattattc agcttcacca tctgcaggtt
     1801 gttcgccgcc atcaattggc tgcgctgcgg gatcttctgc ttgtgggata gttccaacag
     1861 ttgatgcagg gcgattagct tcaggtttaa ta
//
LOCUS       DAORKW010000665         1890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_3995_length_1890_cov_3.882834, whole genome shotgun sequence.
ACCESSION   DAORKW010000665 DAORKW010000000
VERSION     DAORKW010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3995_length_1890_cov_3.882834"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="PLB46_16765"
     CDS             complement(<1..1500)
                     /locus_tag="PLB46_16765"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HRF78233.1"
                     /translation="MSPKPKLNSIRSFYTIFFICFSVSSFVGFTQSPTPDLFIESNIA
                     LIQYGSAVASAGDINGDGYGDVIVGTKYYTNVETNEGKIEIYLGSAVGISPLPSTVIE
                     SNLPNTYYGNSVACAGDINNDGFDDVIVGAYLYDNGQTNEGAVYIYHGSAFGLTIVPA
                     IILESNQASAQFGRSVSTAGDINNDGFDDVLIGSDAYTNGQTYEGRTFVYHGSAAGIG
                     ITPNATMESNLVNASFGRSVAGLGDVNADGFDDIIVGSPNYTNGHSQEGRAYIYHGTA
                     TGINTVPITVMENNKALSYLGFSVSGAGDVNNDGYFDVVLGAYNADNLQDGEGFVYIH
                     YGSASGISATPNIVLESNREAAFFGYSVACAGDRNADGFDDIIIGANEFTNDDVQEGR
                     AFIYYGAASGITDMPNATFESDKSYSNFGAAVSGAGDVNNDGFDDLIIGASDYSDAHS
                     NEGAIYFYLGDNCPTTVFHADSDLDGFGSMISIIVACEMPEGYILDGNDC"
     gene            complement(1554..>1890)
                     /locus_tag="PLB46_16770"
     CDS             complement(1554..>1890)
                     /locus_tag="PLB46_16770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78234.1"
                     /translation="GCFKTSAGIIVTVPCKEGEAISSESTFTISPNPSAGLFTVSAFS
                     YGSLENNTSAQLEIYNAIGQLIYTQVLETTDSQINYNINLQHIASGIYFVRLNTDGLF
                     SEKKLIIQH"
BASE COUNT          620 a          386 c          306 g          578 t
ORIGIN      
        1 acaatcgttg ccatctaaaa tataaccctc aggcatttcg caggcaacaa taattgaaat
       61 catgctgcca aaaccatcaa ggtcgctatc tgcatgaaat actgttgtgg gacaattgtc
      121 gcctaaataa aaataaatgg cgccttcatt ggaatgtgca tcggaataat cgctagcacc
      181 aataattaaa tcatcaaatc catcattatt tacatcacct gcaccggaaa cagcagcgcc
      241 aaaattggaa tacgatttat cgctttcaaa agtggcgttc ggcatatcgg taattcctga
      301 cgctgcacca taataaataa atgctcttcc ttcttgaaca tcatcattgg taaattcatt
      361 tgctccgata ataatatcat caaatccatc tgcattccta tcacctgcgc aagcaaccga
      421 atacccaaaa aaagctgctt ctcgatttga ttccaacaca atatttggtg ttgcacttat
      481 acctgatgcg gaaccataat gaatataaac gaatccttca ccatcttgca aattgtcggc
      541 attataagcg cctaaaacaa catcaaaata gccatcatta ttaacatcac cggcgccgga
      601 aactgaaaaa cccaaatagg aaagtgcttt attattttcc attactgtaa tgggaacggt
      661 atttattcca gtagctgttc cgtgatatat gtaagctcgt ccttcttgac tatgaccatt
      721 tgtgtagttt ggtgaaccga caataatatc atcaaaacca tcagcattta catcccccaa
      781 tccagcgact gagcggccaa atgatgcatt tacaagattg ctttccatag ttgcatttgg
      841 tgtaatgcca atgccggcag cagagccatg atacacaaaa gttctacctt cataagtttg
      901 tccatttgtg taagcatctg agccaattaa tacgtcatca aatccgtcat tattaatatc
      961 gccagctgtg gaaacagacc taccaaattg tgcacttgct tgattacttt caagaattat
     1021 tgccggcacg attgttaacc caaaggcaga gccatgataa atatagacag caccttcgtt
     1081 tgtttgacca ttatcatata aatatgctcc aacaatcaca tcatcaaatc catcgttatt
     1141 aatatcacca gcacaagcta ccgaattgcc gtaataagtg tttggtaaat tactttctat
     1201 tactgttgaa ggaagtgggc taatgcctac agctgaacct aaataaattt ctattttacc
     1261 ttcattcgtt tctacgtttg tataatattt tgtgccgaca attacatctc cataaccatc
     1321 accatttata tctcccgctg atgcaacagc ggagccgtat tgtattaatg caatattact
     1381 ttcaataaaa aggtcaggtg ttggagattg tgtaaaacca acaaatgaac ttacagaaaa
     1441 acagataaaa aatatagtat aaaatgatcg aattgaattt aatttcggtt taggtgacat
     1501 atttaaattg tttacgatta ttgcactatt ttattttata acctattatg taattaatgc
     1561 tgaataatta attttttttc tgagaataat ccatctgtat ttaaacggac aaaataaatg
     1621 ccgcttgcga tatgttgtaa attaatatta taattaattt gactatctgt tgtctccaac
     1681 acttgcgtat aaatgagttg cccgatggca ttataaattt cgagttgagc agatgtatta
     1741 ttttcaagcg aaccataaga gaaagcggaa acagtaaata agcccgcgct tgggtttgga
     1801 gagatggtga atgttgattc agatgaaatg gcttctcctt ctttacacgg taccgtaaca
     1861 ataatccctg ctgaagtttt aaaacaaccc
//
LOCUS       DAORKW010000666         1889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4000_length_1889_cov_4.062159, whole genome shotgun sequence.
ACCESSION   DAORKW010000666 DAORKW010000000
VERSION     DAORKW010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4000_length_1889_cov_4.062159"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1889
                     /locus_tag="PLB46_16775"
     CDS             <1..>1889
                     /locus_tag="PLB46_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78235.1"
                     /translation="TLQRLQYVIGDDGIKYYSTIRKDLATTTTNAEGHYLFNYSDTAH
                     YGLLTLDGMVTYGWGCSELPEYAPDDNPIPDFNDQINEFDNNIIIKERGPNAGDMPSN
                     LSTRSFGGTPWPCVASGALYQELAIVVESGYYTSPATHFTILPAQHKTVAPLTCYVKA
                     YDLELTLIADPDVDVQYKVPGDALRDMLIVLVRNNKPQDVPENEVIVNKPVDGAMVAK
                     LINNAKNLGLYTDYSLSVENVDENDGGYEFVNMNEYIGSGMSIDAEDIVGIGTASAYT
                     FGGDDTPVQITFKNLVKNIGSGDEYQIIAIPDYENSDINYIVPLHTFKSDFTLQNTTH
                     PYGNVTIFGEPSTNTEGVYPKMKMHYVIEASPLNPVIFGKVLRSDNGYPLYDVKVQLK
                     KSTTVGGVINIVEVETTTAADGSYYLIPTAEQYNSSSVQLFSRAITFTKFGYDDNTVS
                     LFPTKKGKISNRVADTLQPGVLVSGYIYLDAWDETLNNYPAVPATVQIGNGPMVHTVC
                     VDQEFNLDGEFLDGYMEQQIPFDPGIFMGDIYEDIYDPLFYDPGTFHFQNDIVNGIDN
                     TEILFGGKTLDLGDTEGLATYSLDVNADVDDGFVIDKPGFEFTEAETETCELAYYATF
                     ASRGT"
BASE COUNT          601 a          312 c          380 g          596 t
ORIGIN      
        1 tacattacaa cgtttgcaat atgtaattgg tgatgatggg ataaaatatt attccactat
       61 tcgcaaagat ttagctacaa ccaccaccaa tgcagaaggg cattatttat tcaactactc
      121 ggatactgcg cattatggtt tattgacttt agatggtatg gtaacttatg gttggggatg
      181 tagcgaatta cctgaatatg cgccggatga taatcccatt cctgatttta acgaccaaat
      241 aaatgaattt gataataata ttattataaa agaacgtgga ccaaatgcgg gcgatatgcc
      301 atcaaatttg tccacacgtt cgtttggtgg cacgccctgg ccttgtgttg ccagcggtgc
      361 cttatatcag gaattagcca ttgttgttga aagcggatat tacacgagtc ccgctacaca
      421 ttttaccata ttacctgctc aacacaaaac ggttgcacca ttaacatgtt atgtaaaagc
      481 atatgatttg gaattaacct taattgcaga ccctgatgtg gatgttcaat ataaagtacc
      541 gggtgatgca ttacgcgata tgcttattgt tttagtacgc aataacaaac ctcaagatgt
      601 tcccgaaaac gaagtaattg taaacaaacc ggttgatgga gctatggttg cgaaattgat
      661 aaataatgca aaaaatttag ggctgtatac ggattattcg ttatctgttg aaaatgtgga
      721 tgaaaatgat ggcggttacg aatttgtaaa tatgaatgag tatataggtt caggtatgtc
      781 gatagatgct gaagatattg tcggcatagg aactgccagc gcatatacat ttggtggcga
      841 tgacacaccg gtacaaatta cgttcaaaaa tttagtaaaa aatattggtt caggcgatga
      901 atatcaaatt attgccatac ctgattatga aaattctgat attaattata ttgttccgct
      961 acatacattt aaatcggatt tcacacttca aaatactact cacccctatg gtaatgtaac
     1021 catatttggc gaaccttcaa ccaatacaga aggtgtttat ccaaaaatga aaatgcatta
     1081 tgtgatagag gcatcacctt taaatcctgt tatttttggg aaggtattac gttcagataa
     1141 tggttatcct ttatatgatg taaaagttca actcaaaaaa agcaccacag ttggtggagt
     1201 gataaatata gtggaagtgg aaacgacaac agcagcagat ggtagttatt atttaatacc
     1261 aactgctgag caatataatt ccagctctgt gcaattattt tctcgtgcca taacatttac
     1321 caaattcgga tatgatgata atactgtttc attatttcca acaaaaaaag gtaaaatcag
     1381 taatcgcgtg gcagacacct tacagcccgg tgttttggtt agcggatata tttatttaga
     1441 tgcctgggat gaaacactaa ataattatcc tgctgttcct gccactgttc aaattggcaa
     1501 tggaccaatg gtacacactg tttgtgtgga tcaggaattt aatttagatg gggagttttt
     1561 ggatggatat atggagcaac aaattccttt tgatccgggt atattcatgg gtgatattta
     1621 tgaagacatt tatgacccgt tattttatga tcctggtaca tttcatttcc aaaacgacat
     1681 tgttaacgga attgacaata ccgaaatatt atttggcggg aaaacactcg atttaggtga
     1741 tactgaaggt ttggctactt attcgttaga tgttaatgct gatgttgatg atggatttgt
     1801 aatagataaa ccgggttttg aatttaccga agctgaaact gaaacctgtg aattagctta
     1861 ttacgccaca tttgcatctc gtggcacct
//
LOCUS       DAORKW010000667         1884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4017_length_1884_cov_4.870421, whole genome shotgun sequence.
ACCESSION   DAORKW010000667 DAORKW010000000
VERSION     DAORKW010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4017_length_1884_cov_4.870421"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..748)
                     /locus_tag="PLB46_16780"
     CDS             complement(5..748)
                     /locus_tag="PLB46_16780"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRF78236.1"
                     /translation="MMNLFIFTPMRYFCLCIGILAFFSCKKQPKTEDENIIDTLEVHT
                     SDSTLEKKLIRAGLVNVQSVIPEIQVELKYSTTDNFLHADVYGDLTNAYLQKDVVEKL
                     KAAYQFLQETHPNLTFIIYDAVRPLSIQYKMWEILDLPPGQKEKYLSNPKNGSIHNYG
                     AAIDMSLAEKNGKPLDMGTPFDFFGEAAEPQLEYKMLQEGVLTQQQIDNRKILRSVMQ
                     KAGFRQLPAEWWHYNSCSREEAKIKYAVI"
     gene            complement(799..>1884)
                     /locus_tag="PLB46_16785"
     CDS             complement(799..>1884)
                     /locus_tag="PLB46_16785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78237.1"
                     /translation="EGVAQCEFGVWDNYWIFKTDFKGRILWQTCVGGNSWDYPYAIEE
                     TPDGNFVVIGSIADDGINISEMHGVKHDIWVVKIANDAPSNQININTFPAQALCPGSE
                     ITVPFAAYGVYNNTNVYSLEISDATGSFASPETVAIIASKASGFHEFETTLPADIVPG
                     GDYKLRVKSSNPPLIGTENQGDITTCPVPASLIDIVLSASSATISWADVNCAETFTVK
                     YKKAIGGSWITVTSTDSVLTLTGLLPETEYKWKVRSDCNASPTDKSAFSLITESFTTL
                     PLKEGDLVMNNFVITPNPTSDWLTIQMDYTTSAYYQLFSTDGKLMLEGTINGSQNTID
                     VSSLNTGMYIIKLNTNLNTQSLNVIIE"
BASE COUNT          579 a          356 c          320 g          629 t
ORIGIN      
        1 tagtttaaat cactgcatat ttaattttcg cttcttcccg cgaacacgaa ttataatgcc
       61 accattccgc aggtaattgt cggaagccgg ctttttgcat aacgctacgc aatatttttc
      121 tgttgtcaat ttgctgttgt gtaagcacac cttcctgcaa cattttatat tccagttgtg
      181 gttctgctgc ttcgccaaaa aaatcaaatg gtgtgcccat gtctaaaggt ttaccatttt
      241 tttcagccaa cgacatatca atggctgcgc cgtaattatg tatactccca tttttggggt
      301 tagataaata tttttctttt tgccccggcg gcagatccaa aatttcccac attttatatt
      361 gtatacttaa tggccttacc gcatcataaa ttataaacgt gaggttggga tgtgtttcct
      421 gcaaaaactg ataagctgct tttaattttt ccactacatc tttttgcaga taagcattgg
      481 ttaaatcgcc atacacatca gcatgtaaaa aattatcggt tgtggaatat ttgagttcaa
      541 cctggatttc aggaataaca gattgaacat taacaagacc ggcgcgtatt aatttttttt
      601 caagtgtaga gtcgcttgta tgaacttcta gcgtatcgat tatattttca tcttcagttt
      661 ttggttgctt tttacaagag aaaaaagcca agatgccgat gcataagcag aagtaacgca
      721 tgggtgtaaa aataaataaa ttcatcatta accgcaattt ataaatgtat tgaggttaat
      781 gatgaattca agtgtaaatt attcaataat aacatttaaa ctttgggtgt ttaaattggt
      841 atttaattta ataatgtaca tccctgtatt taaactgctt acatcaatag tattctgcga
      901 accatttatt gttccttcca acattagttt accatctgtg ctaaataatt gataatacgc
      961 tgaagtagta taatccattt gaatggttaa ccaatcgctt gtcgggttag gggtaattac
     1021 aaaattattc ataaccaaat caccttcttt aagcggtaaa gtggtaaacg attcggtaat
     1081 taaactgaat gctgatttat ctgtaggtga agcattacaa tcggacctta ctttccattt
     1141 atattccgtt tccggtaata aaccggtaag ggttaaaaca gagtcggttg acgttacggt
     1201 aatccaactt ccaccaattg cttttttata ttttacggta aatgtttctg cgcaatttac
     1261 atcagcccaa cttattgttg cactgcttgc tgataaaaca atatcgatta aagatgcagg
     1321 aacaggacat gttgtaatat caccttggtt ttcggtacct attagtggag gatttgaact
     1381 ttttacacgt aatttataat caccaccggg aacaatatct gccggtaatg tagtttcaaa
     1441 ttcatgaaat ccgcttgctt tggatgcaat gattgcaact gtttccggtg aagcaaaact
     1501 acctgttgcg tcagaaattt cgagactgta aacattcgta ttgttataca caccataagc
     1561 agcaaatggc acagtaattt ccgaaccagg gcataaagcc tgagcaggaa aagtattaat
     1621 atttatttga ttggaaggag catcattggc aatttttaca acccaaatat catgtttaac
     1681 gccatgcatt tcgctgatat taatgccatc atctgcaata gagccaatta caacaaaatt
     1741 gccatcaggt gtttcttcaa ttgcgtaagg ataatcccaa ctatttccac ccacacatgt
     1801 ttgccaaaga atacgacctt tgaaatctgt tttaaaaatc caataattat cccaaacacc
     1861 aaattcgcat tgcgcaacac cttc
//
LOCUS       DAORKW010000668         1882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4023_length_1882_cov_3.940887, whole genome shotgun sequence.
ACCESSION   DAORKW010000668 DAORKW010000000
VERSION     DAORKW010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4023_length_1882_cov_3.940887"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..>1882
                     /locus_tag="PLB46_16790"
     CDS             303..>1882
                     /locus_tag="PLB46_16790"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF78238.1"
                     /translation="MKSFKTPIVIIGMLVLVYSCGSNKNGKEYLNEWKFTENIDKAKI
                     VPTDQETGKMFDILTSKTTGINFNNELLETFDLNYYRYGYSYNGAGVGVGDFNNDGLQ
                     DLFYTGNVVPDRIFLNKGNMKFEDVTAKSGINQKPGWSTGVTVVDVNEDGFKDIYVCR
                     ARFEDPEKRRNLLYINNGDMTFTEKAKEFGLDDDSYSTHANFFDADNDGDLDVYIVTH
                     PTDFKDKNKQKNYQKIEEGTNTSNRFYRNDGNGKFTECHKEVGINNHGFGLSVTTGDI
                     NNDGWMDVFVGNDYIMHDYTYLNQGHGTFKESSREVLNKTSYYGMGTDIADYNNDGLM
                     DMFTVDMDIEGNYGTKTFMQSNKQTFLRTLVNGGYLQQYGRNALQLNNGVGKFSEVAN
                     FAGVSTTGWSWSPLFADYDNDGFKDLYVANGFLKDSHMDIMEVYIKLTRANRLSDSTE
                     YHELRKQIPENSVLEWPNAMFRNKGDLTFDDVREEWGMYFPSTSYGAAYADFDNDGDI
                     DIVLNNANMETYIARNNST"
BASE COUNT          623 a          349 c          375 g          535 t
ORIGIN      
        1 gtaggaattt ggcatcaatt tcgcttgatg gtgtagacgc atcgctaaac aatgggtttt
       61 ctccacctcc ggattgtttt ccgcatccgg cagcaaacag aataacaccc gatagcaata
      121 ctacagttaa gtgtttgaat ctcatgactc tagaaattta gttaagtttt attggattta
      181 aataaactcc gcaaaattaa tcattttacg gcagctttaa ttcgttaaca aaacatttac
      241 agaaagggtg ctttcatgcc tttataatgg ggttaacttt gcagcttaaa ccgtaataag
      301 gcatgaaatc tttcaaaaca ccaattgtaa ttatcggtat gctcgttctg gtttatagct
      361 gcggctctaa taagaacggg aaggaatacc tcaatgaatg gaaatttact gaaaacatcg
      421 acaaggctaa aatagtgcct accgaccagg aaaccggtaa aatgttcgac attttaacca
      481 gtaaaactac cggcattaac ttcaacaatg aacttctaga aacctttgac ctcaactact
      541 accgttatgg ctattcctat aatggcgcag gtgtaggtgt gggcgacttt aacaatgacg
      601 ggttacagga tttattctat accggtaacg tagttcctga ccgtattttc ctcaacaaag
      661 gcaatatgaa atttgaggat gttactgcca aatccggcat taatcagaaa ccgggctgga
      721 gcacaggtgt tacagttgtt gacgtaaacg aagatggttt taaggatatt tacgtgtgtc
      781 gtgcgcgttt tgaagatcct gaaaaacgca gaaacttgct ctacatcaac aacggtgata
      841 tgacttttac tgaaaaagca aaagaattcg gacttgatga cgacagctat tctacacatg
      901 ctaacttttt tgatgcagat aatgatggtg accttgatgt ttacatcgta acccacccta
      961 ccgacttcaa agacaaaaac aaacagaaaa attatcagaa aattgaagaa ggtacaaata
     1021 ccagtaaccg tttttaccgc aacgatggca atggtaaatt taccgaatgc cataaagagg
     1081 tgggtataaa taaccacgga ttcggattat ctgttacaac cggcgatatt aacaatgatg
     1141 gatggatgga tgtatttgtt ggtaacgact atatcatgca cgattacact taccttaatc
     1201 aaggtcacgg tacttttaaa gaaagcagcc gcgaagtatt aaacaaaact tcctattatg
     1261 gtatgggtac cgatattgcc gattataaca atgacggttt aatggatatg ttcacagtgg
     1321 acatggatat tgaaggaaac tatggtacta aaaccttcat gcaatcaaac aaacaaacct
     1381 ttttacgtac tttagtgaac ggcggatatt tacaacaata cggccgtaat gcattacaat
     1441 taaataatgg cgttggtaaa tttagtgagg tagccaactt tgcaggtgtt agtaccaccg
     1501 gctggagctg gagcccttta tttgccgatt acgataacga tggatttaaa gatttatatg
     1561 tggcgaatgg tttcttaaaa gacagccaca tggatatcat ggaagtgtat atcaaactca
     1621 ctcgcgctaa ccgtttatct gattcaactg aatatcatga actcagaaaa caaattcctg
     1681 aaaattcagt actcgaatgg ccaaacgcaa tgttccgcaa caaaggtgat ttaacttttg
     1741 acgatgtacg tgaagaatgg ggtatgtatt tcccatccac ttcttacggt gcagcatatg
     1801 ccgatttcga taacgatggt gatattgata tcgtattaaa taacgcgaat atggaaacat
     1861 atatcgcaag aaataattca ac
//
LOCUS       DAORKW010000669         1881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4028_length_1881_cov_2.991785, whole genome shotgun sequence.
ACCESSION   DAORKW010000669 DAORKW010000000
VERSION     DAORKW010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4028_length_1881_cov_2.991785"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="PLB46_16795"
     CDS             <1..1780
                     /locus_tag="PLB46_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRF78239.1"
                     /translation="FIKKYIDYLAMYKMNTFHWHLTDDQGWRIEIKQYPKLTEIGAWR
                     DGTMVGHYRDQQYDNVRYGGFYTQKEIREIVRYATDRHITIIPEIEMPGHCMAALAAY
                     PELGCVSDTTYQVAKGWGVVEDVFCPKDSTFILLENVLEEVMALFPSPYIHTGGDEVE
                     KIRWRNCSHCQQLIKEKGLVNEHGLQSYFIQRIEKYVNSKGRSIIGWDEILEGGLAQN
                     ATIMSWRGEEGAVLAAQQQHYAIMTPGSHCYFDYYQGAPQLEPLAIGGFVPLDKVYAF
                     NPIPASLTPEQATYILGAQANIWTEYISFPEQVEYMLLPRMLALSEVLWTSNENKNYD
                     DFLTRLLFHFQLLDKTNTNYAKSIYQINYKVTPASELNKLNITLLSNPLLGKIEVQTV
                     NVLNGNKNNFTYQNSFTVDAETPYINAQLTGSIKNTPTTHIKLKFNKATGKSITLKNE
                     PSKKYSADGPFTLVNGVEGTVNPSWNGNEWLGFLGSDLQAIIDLGEVDTIKSVTVGCL
                     FDKLSWIYPAESVQIWISTNGKNYEQAGKIKYNGDQKRNTITVNSSIKTGRYIKIEAI
                     NLGNIPSGNPGAGSKPWLFIDEITVE"
BASE COUNT          665 a          310 c          352 g          554 t
ORIGIN      
        1 atttattaaa aaatatatcg attatttagc catgtataaa atgaatacat ttcattggca
       61 tttaaccgat gatcagggtt ggagaataga aataaaacaa tatcctaaac ttaccgaaat
      121 tggcgcatgg cgcgatggca caatggttgg tcattatcgc gaccagcaat atgataatgt
      181 tcgatatggt ggattttata cacaaaaaga aataagagaa attgtcaggt atgctaccga
      241 caggcatatt accattatcc ctgaaattga aatgccggga cattgtatgg ctgcattagc
      301 tgcttatcct gaacttggtt gtgtgagtga taccacttat caggtagcaa aaggttgggg
      361 tgttgttgaa gatgtatttt gtccaaaaga ttccaccttt attttgttag aaaatgtttt
      421 ggaggaagtg atggctttat ttccttctcc atatatacat actggcggag atgaagtgga
      481 aaaaattcgc tggagaaatt gcagtcattg tcaacaatta attaaagaaa agggattagt
      541 taatgaacat ggtttacaaa gttattttat ccaacgcatt gaaaaatatg tcaacagtaa
      601 aggcagaagt attatcggtt gggatgaaat tttagaagga ggccttgcac aaaatgctac
      661 cattatgagt tggcgtggtg aagaaggtgc tgtattagct gcacaacaac aacattatgc
      721 catcatgaca cccggcagcc attgttattt cgattattat cagggagctc cgcaattgga
      781 accattggct attggcggat ttgtaccttt agataaagtg tatgcgttta atccaattcc
      841 tgcttcacta acacctgaac aagcaactta cattttaggc gcgcaggcaa atatctggac
      901 cgaatatatc agtttccccg aacaagttga gtatatgtta ttaccaagaa tgctcgcatt
      961 gagtgaggta ttgtggactt ccaatgaaaa taaaaattat gatgattttt taaccagatt
     1021 attatttcac tttcaattgt tggacaaaac aaatactaat tacgccaaat caatttatca
     1081 gataaattat aaagtaacgc cagcaagtga attaaataaa ttaaacatta cattattatc
     1141 taatcctctc ttaggaaaga ttgaagtcca aactgtcaat gttttaaatg gcaacaaaaa
     1201 taatttcacc tatcaaaatt catttacagt tgacgccgaa acaccttata tcaatgcaca
     1261 attaaccggc tctataaaaa atacaccaac tacacacatc aaattaaaat ttaataaagc
     1321 aaccggtaaa agtattacct taaaaaatga accctccaaa aaatatagtg ctgatggtcc
     1381 atttacttta gtaaacggtg ttgaaggaac cgtaaaccca agttggaatg gtaacgaatg
     1441 gctagggttt ttagggtctg atttgcaagc aattattgac cttggtgagg tagataccat
     1501 taagtccgta acagtaggtt gtttatttga taagttaagt tggatttatc ctgctgaatc
     1561 tgtccaaatt tggatttcaa ccaatggaaa aaattacgaa caagcaggta aaataaaata
     1621 caatggcgat caaaaacgaa ataccattac tgtcaattca tcaattaaaa cagggagata
     1681 tattaaaatt gaagcaataa atttgggcaa cataccttcc ggtaatccgg gagccggttc
     1741 taaaccctgg ttatttattg atgaaataac cgtagagtaa ttaattttaa tcagtacaag
     1801 tatcacacta tggcaagcag aagaaatttt ataaaagcag ctgcactcag cgcaggcgca
     1861 cttaccagca gtaaaacatt t
//
LOCUS       DAORKW010000670         1869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4057_length_1869_cov_4.068908, whole genome shotgun sequence.
ACCESSION   DAORKW010000670 DAORKW010000000
VERSION     DAORKW010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4057_length_1869_cov_4.068908"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLB46_16800"
     CDS             <1..188
                     /locus_tag="PLB46_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78240.1"
                     /translation="EVFGVYYPNCIIQIDETGDSKFPLLENRYIQNQTQIFLCRNNTC
                     SLPVSNVQDFTELLNKF"
     gene            268..1263
                     /locus_tag="PLB46_16805"
     CDS             268..1263
                     /locus_tag="PLB46_16805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HRF78241.1"
                     /translation="MLRICIVTLLLIVSAGLYAQSTQLYSYGQQDPQFTQYMFNPSSF
                     NPAAAGEMDAWVSTLNVRNQWVNLPGAPISQIFTSHIPIYKISSGAGLAVMNDIAGQQ
                     RNTGLTAMYAFHKRFRTSTLSMGVSGGFVQQQLNGGKLITPTGNYEGIVDHNDPNLPV
                     TTKTELLPDAAAGLYFFGKNLKIGASATHLLLPLQASKETAETDVQYNPNAYVYVGYD
                     IDVTDRLIITPSVLYKTDLKASIVDINTIFTFNQNILAGASFRGYANSRYDAAALIAG
                     LNVTDNWRISYSYDITLSELNSVSAGSHEVVLRYSIPVARPRAGKMINNPRYLYH"
     gene            1411..>1869
                     /locus_tag="PLB46_16810"
     CDS             1411..>1869
                     /locus_tag="PLB46_16810"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRF78242.1"
                     /translation="MKRNLPLLTFACVAIIFGSGCSSSGWGDYRGQLLGMQDRPEWEP
                     VNPFGMVYIPSGTLHIGPSDQDVNNSLWQRTKSISIQGFYMDDAEITNNEYRQFVFWV
                     RDSISHVLLDHYQEEEEGQETEVPLLDWDEEIDWDDEETRPRLEELSLPAS"
BASE COUNT          569 a          354 c          398 g          548 t
ORIGIN      
        1 atgaagtttt tggcgtttat tatccaaatt gtattatcca aatagatgaa acaggagata
       61 gtaaatttcc tttactagaa aatcggtata tccaaaacca aacacaaatc tttttatgcc
      121 gcaataacac ttgttccctg cctgtcagca atgtgcaaga tttcaccgaa ctgttaaata
      181 aattttgagt gctgcattag ttgaattaac tttagctttt atacgaaatg ccgattgttg
      241 gcgtttatga aaaaatggtg tagctgaatg ttgcgaattt gtattgtaac tctcctctta
      301 attgttagtg ctggacttta tgcccaaagc acgcagttgt atagttacgg gcaacaagac
      361 cctcaattta cgcagtatat gtttaaccca tcttctttca atccggcagc tgcaggagaa
      421 atggatgcat gggtatcaac actcaatgtg cgtaatcagt gggtaaatct accgggtgct
      481 cccatctcac aaatttttac ttctcatatt ccaatttata aaatttcaag tggagcaggt
      541 ttggcagtaa tgaacgacat tgcaggtcaa caacgcaata caggattaac ggcaatgtat
      601 gcattccata agcgtttcag gacatcaacc ctgagtatgg gcgtaagtgg aggttttgtt
      661 caacagcaac ttaatggggg caaattaata acaccaacag gtaattatga gggtattgta
      721 gaccataatg acccaaattt gcctgtcaca actaagaccg aattgttacc cgatgccgct
      781 gcagggctct atttctttgg gaaaaattta aaaatagggg cttcggctac ccatttactg
      841 cttcctttgc aggcaagtaa ggaaactgcc gaaaccgatg tacaatataa tccgaatgct
      901 tacgtttatg tgggatatga cattgatgtg acagatcgat tgattattac ccctagcgtt
      961 ctttataaaa ccgacctaaa agcgtcgatt gtggatatca atacaatatt tactttcaac
     1021 caaaatattt tggccggtgc gtcgtttaga ggttacgcca acagtcgcta tgatgcagca
     1081 gcattgattg ccggcttaaa cgtaacagat aattggcgaa ttagttattc atatgacatt
     1141 accttatcgg agttaaattc ggtaagtgca gggtcacatg aagtggtgtt gagatacagt
     1201 attccggtgg cgaggccacg tgcgggaaaa atgataaata acccgcgata tttgtatcat
     1261 taataggctg aaactttgca attttgcaga aagttggtaa tttcagcccg cttttgaaat
     1321 tagtgtgtgt ataaactgtg gtaaaatact attgaaattc aataaaaaac ctatttttac
     1381 acggatttaa ttgtacaaat catatcaagt atgaaaagaa acctgccatt gttaactttt
     1441 gcatgcgtag ccatcatttt tggaagtggt tgtagctcct ctggttgggg cgattaccgc
     1501 ggacaattgt taggtatgca agaccgtcct gagtgggaac cggtaaaccc ttttggaatg
     1561 gtttacatcc cgtcgggaac attacacatc ggtccgagcg accaggatgt gaacaattct
     1621 ctctggcaac gaacgaaatc catctctatt caagggtttt acatggatga tgcagagatt
     1681 accaacaacg aatacagaca attcgtattc tgggtaagag actccatctc gcacgtatta
     1741 ctcgaccact atcaggaaga agaggaaggt caggaaactg aagttccact tttagactgg
     1801 gatgaggaaa ttgattggga tgatgaggaa acacgcccac gccttgaaga actttcttta
     1861 cctgcaagt
//
LOCUS       DAORKW010000671         1855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4108_length_1855_cov_5.507222, whole genome shotgun sequence.
ACCESSION   DAORKW010000671 DAORKW010000000
VERSION     DAORKW010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4108_length_1855_cov_5.507222"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /gene="xseA"
                     /locus_tag="PLB46_16815"
     CDS             <1..1225
                     /gene="xseA"
                     /locus_tag="PLB46_16815"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRF78243.1"
                     /translation="VAGAQKIKLFETETGQQFTNGLQVLVKVKLEFHKVFGMQLVLLD
                     IDPTFTLGKLELQRRETLFKLATHHAEIIQLIGDTYVTRNKQTPFNAVIQCIAIIGSP
                     NSEGYIDFTHTIANNQFGYKFVIDIYQSTVQGGDAEFELIQCLIAIHKSTKKYDAVVI
                     IRGGGAKSDFLVFDKYKLSLAIAKFPIPVITGIGHTNDVSIVDMMAHTNTKTPTKAAE
                     FIISHNRQFEEDIMALQKTAVIKSQQLLSKHIQFIHATNTIIINQSRLYIGLHKDALN
                     GIQQIVTNKTKGLLYHLQTDLVNLLNQILSQPKITTANKQNELNNQINNIKIFSHKYL
                     VNQKGYVGHFVSLINMMRPENILKKGFAIIKQNGVIISSSDKIKQGDELSITMEKHII
                     STTVNSKSDNEGTNL"
     gene            1206..1415
                     /gene="xseB"
                     /locus_tag="PLB46_16820"
     CDS             1206..1415
                     /gene="xseB"
                     /locus_tag="PLB46_16820"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRF78244.1"
                     /translation="MKELTYEAAYAELKQIAAEIENESVSVDVLAEKVKRASELITFC
                     QTKLRSTEQEVNNIIKQMETKTTPS"
     gene            1514..>1855
                     /locus_tag="PLB46_16825"
     CDS             1514..>1855
                     /locus_tag="PLB46_16825"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78245.1"
                     /translation="MKHFTLLIFAAFLFTIQITNAQHIAEGGSFTIVLCADGTAQAWG
                     ANYVGQLGDNGTGDNSSVPVDVYGIDNLKAIASGTTFSLALKNDGTVWAWGENTNGGC
                     GDGTKVTRFAPV"
BASE COUNT          645 a          326 c          340 g          544 t
ORIGIN      
        1 ggtggcggga gcgcaaaaaa ttaaattgtt tgaaaccgaa accgggcaac aatttaccaa
       61 tggtttacag gtattagtaa aagtgaaatt agagtttcat aaagtatttg gtatgcaatt
      121 ggttttgctc gacattgacc cgacatttac tttagggaaa ctggaattac aacgtagaga
      181 aacacttttc aaacttgcta cgcatcacgc cgaaataatt caattaattg gcgacacgta
      241 tgttaccaga aataaacaaa caccatttaa tgccgtaatt cagtgcatag ccattatcgg
      301 ttctccaaat tctgaagggt atatcgattt tacgcatacc attgccaata atcaattcgg
      361 atataaattc gttatcgaca tttatcaatc cacggttcag ggtggcgatg ctgaatttga
      421 attaattcaa tgtttaattg ctattcataa atcgacaaaa aaatatgatg ccgttgtgat
      481 tattcgcggc ggtggcgcaa agtccgactt tttagttttt gataaatata aattatcatt
      541 ggccattgca aaattcccga tacctgtgat tacgggaatt ggtcacacaa acgacgtgag
      601 cattgtggat atgatggcgc ataccaatac caaaacccca acaaaagctg cagaatttat
      661 tatctctcac aaccgtcagt tcgaagaaga tatcatggca ttacaaaaaa cggctgtcat
      721 taaatcacaa caattattga gcaaacatat acagtttata cacgctacca atacgattat
      781 cattaatcaa tcgcgattgt atatcggttt gcacaaagat gcgttaaatg gtattcagca
      841 aatagttacc aataaaacca aagggttatt atatcattta caaaccgatt tggtaaattt
      901 attaaatcag attttatctc aacccaaaat caccacagcc aacaaacaaa acgaattaaa
      961 caatcaaatc aataatatta aaatattttc gcataaatac ctcgtcaacc aaaaaggata
     1021 tgttggccat tttgtaagtt taataaacat gatgcgtcct gaaaatattt tgaaaaaagg
     1081 atttgccatt atcaaacaaa atggcgttat tatcagcagt agcgataaga ttaaacaggg
     1141 tgatgaatta agtatcacta tggaaaaaca tattatttca acaaccgtaa attcaaaatc
     1201 tgacaatgaa ggaactaact tatgaagcag cgtatgccga actcaaacaa atagcggcag
     1261 aaattgaaaa cgaatcagta tctgtagatg tgcttgccga aaaggtaaag cgcgcatctg
     1321 aactcattac tttttgccaa accaaattgc gctctactga gcaggaggta aataatatta
     1381 ttaaacagat ggagactaaa acaactcctt cctagtttga ctaatttgca ttgcccgggt
     1441 tttgcttatt ttaagagtaa taagttgcaa ttgaattaaa atttgtatac ttttaatctt
     1501 caaaatttcc attatgaaac attttaccct acttattttt gctgcgtttt tatttacaat
     1561 acaaataaca aatgcccaac atattgcaga aggtggcagt tttaccatcg ttttgtgtgc
     1621 agatggtact gcacaggcat ggggggcgaa ttatgtcgga cagttgggtg ataatggcac
     1681 tggggacaat tcatctgttc cggtcgatgt gtatggtatc gacaatttga aagccatagc
     1741 atcaggtaca actttttcat tggctttaaa aaatgatgga acagtatggg cttggggcga
     1801 gaatacgaat ggcggctgtg gagatggaac aaaagtaacc cgttttgctc cagtt
//
LOCUS       DAORKW010000672         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4123_length_1852_cov_4.933222, whole genome shotgun sequence.
ACCESSION   DAORKW010000672 DAORKW010000000
VERSION     DAORKW010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4123_length_1852_cov_4.933222"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..634
                     /locus_tag="PLB46_16830"
     CDS             86..634
                     /locus_tag="PLB46_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78246.1"
                     /translation="MPPLFSNTLTFSDPSEITFTCSSNELRVFVKGTLLGSINTDYPV
                     TMYVTVNNGVKSLTITQISNSVYTQGNTNNPMLDTVYYQFNNRNHAPCMVGCSTSPTT
                     YKIYGWDDEFTGQACVGCPSFTSYFNISGNCMFKAKIEQVMVNNQPKMRLVARYSGSV
                     YLNVNAGIADCFNSPYCYACNV"
     gene            complement(665..>1852)
                     /locus_tag="PLB46_16835"
     CDS             complement(665..>1852)
                     /locus_tag="PLB46_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78247.1"
                     /translation="GLTELRVKALRRAKELAYKFDLTYMLVAILQRERVFAIRYSGGD
                     PVAKINLIHEEEQRVFKSLNNEAELGKIYYSLYAQFVIDPKLNDPQKIDWLVNYKNHP
                     LLQDIDAIDTYYSKFIFIKCHGFILRFSNDYQGLYQNNLALLKLMEQYPQHHFNVMGN
                     YMDAIAAVLAAAHNVGKYEEFEGLLKKLDELPKDKLKDEATIAMNILHYKMLYYMNTA
                     QFDKSEEIINECEHVLKKYSNQVSDSQFISDVYNLSLFLFCKGDIHKSLHYCQEVLNF
                     KTESKQDLQHGAQMLHLLLHYELGNTVYLDSALRNGIRSMQNKSRFNDFEKSFTGYIK
                     KLIKTAERDKMSVYKEMYDFYLAMHQQNNHKRLVLIEETLAWAVSKVEKKPMAETIFY
                     KYK"
BASE COUNT          571 a          331 c          317 g          633 t
ORIGIN      
        1 ctttttctgc tgcaggcctt cattgtgcaa ttcgtgcaat ttggaaattg caaggaatta
       61 actaagattt gcaaaaaaac aaaccatgcc accattattc tcaaacacac tcacattttc
      121 agacccaagt gaaatcacat ttacatgttc atctaacgaa ctaagggttt ttgtaaaagg
      181 tactttatta ggttcaatta atactgatta tccggtaacc atgtatgtta cggtaaataa
      241 cggtgttaaa agtttaacca ttacccaaat cagcaattcg gtatatacgc agggtaatac
      301 taataaccct atgttagata ccgtttatta tcaatttaac aatcgtaatc atgcgccatg
      361 tatggtgggt tgtagtacat cacccactac ttataaaata tatggttggg atgatgaatt
      421 tacaggacaa gcatgtgtag gatgtcctag ttttacttct tattttaata tcagtggcaa
      481 ttgtatgttc aaagcaaaaa ttgaacaggt tatggtgaat aatcagccaa aaatgcgatt
      541 ggttgcccgt tattcaggta gtgtatatct caatgttaac gcagggatag cggactgttt
      601 caacagccct tactgttatg cctgtaatgt ataatgcata agggtggttg ataatcagcc
      661 acccttattt gtatttataa aatatggttt ctgccattgg ttttttctcc acttttgaaa
      721 cggcccaggc gagtgtttct tcgatgagta ctaaacgttt atggttattt tgttgatgca
      781 tagctaaata aaaatcatac atttctttgt agacactcat tttatcacgt tcggctgttt
      841 taattaattt tttgatatag ccggtaaacg atttttcgaa gtcgttgaag cgcgatttat
      901 tttgcatgct gcgaatacca ttgcgcaaag ccgaatccag atatacggta ttacccaatt
      961 cgtaatgtaa caataaatgt aacatttgtg ccccgtgttg taaatcttgt ttagattctg
     1021 ttttaaaatt taacacttcc tgacaataat gtagtgattt atggatatcg cctttacaaa
     1081 ataaaaataa actcaggtta tatacatcag aaataaattg ggaatcggaa acctgattgc
     1141 tatatttttt cagcacatgt tcgcattcat taataatttc ctccgattta tcgaattgag
     1201 cagtattcat gtaatacagc attttataat gcaatatatt catggcaata gttgcctcat
     1261 cttttaattt atctttcggt aactcgtcta gttttttgag taagccttca aactcctcgt
     1321 atttccccac attatgtgca gcagctaaaa cggcagcaat agcatccata taatttccca
     1381 tcacattaaa atgatgttga ggatattgct ccatgagttt taataaggct aaattatttt
     1441 gatacaatcc ctgataatcg ttgctgaagc gaaggataaa tccatgacac ttgataaaaa
     1501 taaatttact gtaataggtg tcaatggcat ctatatcctg aagtaaggga tgatttttat
     1561 aattaaccag ccaatctatt ttttgtgggt cattgagttt agggtcaata acaaattgtg
     1621 catataacga ataataaatt ttccccagtt cagcctcatt attcagactt ttaaatactc
     1681 gttgttcttc ttcgtgaatt aaattgattt tagccacagg atcaccaccc gaataccgaa
     1741 tagcaaacac gcgttcgcgc tgcaaaatgg ccactaacat ataggtgagg tcgaatttat
     1801 aggctagctc ttttgctctg cgcaatgctt ttacacgcaa ttcggttaat cc
//
LOCUS       DAORKW010000673         1840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4167_length_1840_cov_3.546779, whole genome shotgun sequence.
ACCESSION   DAORKW010000673 DAORKW010000000
VERSION     DAORKW010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4167_length_1840_cov_3.546779"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PLB46_16840"
     CDS             complement(<1..596)
                     /locus_tag="PLB46_16840"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HRF78248.1"
                     /translation="MPDTIDYSASKVDGSGNIYVTTNTISATEKANILTTKYNSAGVV
                     QWEVEKDNTDENDYGSAIEVDGSGNVYVGAATWVDGTNKYDYLVIKYNSSGTQQWTAT
                     YNGPGNFYDIPTDIYVDGSGNVYVTGYSYGSTTLGDFCTIKYNSSGTAQWTSRYDYAL
                     DQDAAAIVRSGPGSTVVVIGASENTPGDWDFAAVQYNA"
     gene            1459..>1840
                     /locus_tag="PLB46_16845"
     CDS             1459..>1840
                     /locus_tag="PLB46_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78249.1"
                     /translation="MNKIITLIFVVFALIRPVAAQSFLQMGLDIDGEAAEDEAGTSVC
                     MPDGNTIAIGAYLNDGSSLNVGHVRIYSWNGSEWLQKGEDIDGEADQDRLGWSLDMPD
                     NNTIAIGAYLNDGSEINTGHVRIYN"
BASE COUNT          589 a          325 c          319 g          607 t
ORIGIN      
        1 ctggcattgt attgtacagc agcgaagtcc caatctccag gggtattttc acttgctcca
       61 atcactacaa cagttgaacc tggaccgctt cttacaattg ccgctgcatc ttgatccaat
      121 gcgtaatcat atcttgatgt ccattgtgct gttccagatg aattatattt tatggtgcaa
      181 aagtcaccga gggttgtgct accataacta tatcctgtca cataaacatt tccagaacca
      241 tctacataaa tatctgtcgg gatatcatag aagttccctg ggccattata agtagcggtc
      301 cattgttgtg ttccagatga gttatattta attaccaaat agtcatattt gtttgttcca
      361 tccacccacg tagctgcacc cacatataca tttcccgaac catcaacttc aattgccgaa
      421 ccataatcat tttcatccgt gttatccttc tctacttccc attgaactac acccgctgaa
      481 ttatattttg tcgttaaaat atttgctttt tctgtagcag atatggtatt agttgttaca
      541 tatatattac cagagccatc aaccttagat gcactataat ctatagtgtc aggcatgcct
      601 gatgtttgca cccattcttc tactaaataa atttgcccgc ttacatttgc cgtaacaaaa
      661 aagcaaataa gaattgcgat tttcggaaag ttgagtaata aagttttcag aataattatt
      721 ttgtggatta aataaatatt taatgaaaat tataaaccag tgaatgaatt acaaaattta
      781 aagtgttaaa gttgagtcat ttttcacaaa ttcacggttt tccgtgaatc ctaaaaaata
      841 gacactcata atattttttt ttaatttaaa caatcattat agtggttgtg agctttaatt
      901 aaggaaataa aatcacttca caaaagtatt atgtcctgca caattttttc aggaatttcc
      961 ggcataaaca caaaaatcag cgctcataaa gcccgcgctt tattccgttc ctcctgaaaa
     1021 atatgtacca gccataatag gcaagtttct ttctttttat ttttttctct tttttatttt
     1081 tcttttggaa attactgtct ggagaaaaag aataaaaaag caagggctaa gaaggactct
     1141 gataaccttc tctatcccca tgaaaacata agctaagatt attgaatttt tcgaaatcca
     1201 agataacgca aataaatatt tttacatttt caactccttt caccatctgc taagtttaaa
     1261 gatgcgcttc taattgaaaa aaactctccc tgtaggattg tcttcaaaga atagtatggc
     1321 tcttaaaaac gcagctattc tattaataca ggcagttata agtatcaccc tattgtggta
     1381 tatccagcaa acttcgcctc attaaatttg taaatagttt ttttacatga aataagaaaa
     1441 agggtaaaac ttgataaaat gaataaaatt ataaccttaa tatttgtggt attcgctttg
     1501 ataagaccag ttgcagcaca aagtttttta caaatgggat tggatattga tggggaagca
     1561 gctgaggatg aggcggggac ttcggtgtgt atgcctgatg gtaatacgat tgcaataggt
     1621 gcttatctaa atgatggaag cagtttaaat gttgggcatg ttagaattta ttcgtggaac
     1681 ggcagcgaat ggttgcaaaa gggagaagac attgatggag aggcggatca agaccgtttg
     1741 ggttggtcgt tggatatgcc ggacaacaat acgattgcaa ttggtgctta tttaaatgac
     1801 ggaagtgaaa ttaatacagg tcatgtaaga atttacaatt
//
LOCUS       DAORKW010000674         1837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4174_length_1837_cov_5.595960, whole genome shotgun sequence.
ACCESSION   DAORKW010000674 DAORKW010000000
VERSION     DAORKW010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4174_length_1837_cov_5.595960"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLB46_16850"
     CDS             <1..318
                     /locus_tag="PLB46_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRF78250.1"
                     /translation="KYLEENYRDKISVEKLATELNIGRRNFDRRFIKATGFTPLDYLQ
                     RVKIEAAKKLFETTRNTVNEIMYEVGYKDAKAFRDVFNRITGLSPLEYKSKYNKEGRL
                     SMV"
     gene            complement(402..1508)
                     /locus_tag="PLB46_16855"
     CDS             complement(402..1508)
                     /locus_tag="PLB46_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF78251.1"
                     /translation="MTNMGLKQLFKTTNLILVFLILLCSSCAGQANEKSVNDTLVNEE
                     NSQPISVKQSTVFPQIHTNLNGMVSEFVRAMYQDKKGNYWFGTNGNGIIRYDGATLER
                     VNIEQSPKWLSVRKIVEDEIGNIWFGTSSGLIKYDGKNYTIFSTAAGLQHEEIWGLAV
                     DAKGIIWVGSVGGVSHFDGNKFTPFLLPNSIVENAAPMLSIKSAGGFVEDNDGYMWIN
                     NDGNGIFKYKHGTFIHLTNKNGLTDNNAGVGLKDSKGNIWIGSFNGGASKFDGSTFTN
                     YTKEGVIKGMETGSFYEDSKGNIWFTAENIGVYKYDGTTFTLYTTENGLTSNVVQSIC
                     EDNKGQVWFGTWQGLCIFDGEQFVNARDKEPWTK"
BASE COUNT          571 a          360 c          269 g          637 t
ORIGIN      
        1 aaatatctcg aagaaaatta tcgcgataaa atatccgttg aaaaactggc aactgaatta
       61 aacattggcc gcagaaattt tgatcgacga tttattaaag caacaggttt tacaccattg
      121 gattatttgc aacgtgtaaa aattgaagca gctaaaaaac tatttgaaac tacccgcaat
      181 acagtaaatg aaattatgta tgaagtgggt tataaagatg caaaagcatt tcgggatgtg
      241 tttaatcgta ttacaggttt gtctccgtta gagtataagt caaaatacaa taaagaagga
      301 agattgtcga tggtgtagag tgttttgtaa aaattcaagt gtatttaaaa tttaatcttt
      361 aaaccaccaa ttttttgcaa gtggcttttg caacaatcaa tttatttagt ccaaggttcc
      421 ttatctcgag cattaacaaa ttgctcacca tcaaaaatac acaagccttg ccaggtgcca
      481 aaccaaactt gtcctttgtt atcttcacat atactttgaa caacatttga cgttaatccg
      541 ttttcagtgg tgtataaagt gaatgttgtt ccatcatatt tataaacccc tatgttttca
      601 gctgtaaacc aaatatttcc tttactgtct tcataaaaac tgccggtttc cattcctttt
      661 atgactcctt cctttgtata atttgtgaat gtgcttccgt caaatttact tgcgcctcca
      721 ttaaatgagc ctatccaaat attccccttg ctgtccttta aacctacacc ggcattatta
      781 tctgtaagcc catttttatt tgttaaatga ataaatgttc catgcttata tttgaaaatt
      841 ccatttccat cgttattaat ccacatatat ccatcattat cctcgacaaa acctccggca
      901 ctttttattg aaagcatagg tgctgcattt tctactattg agttaggtaa taaaaacggc
      961 gtaaatttat ttccatcaaa atgactaact cctcccactg aaccaaccca aattatccct
     1021 tttgcatcca cagccagacc ccaaatttcc tcatgttgca aacctgctgc agtagaaaag
     1081 atggtataat ttttcccatc atattttatt agtcctgaag acgtgccaaa ccaaatatta
     1141 ccaatttcat cttcaacaat ttttcttaca gacaaccatt ttgggctttg ctcaatgtta
     1201 actctttcaa gtgtagcacc atcataacgg ataatgccat taccatttgt tccaaaccaa
     1261 taattccctt ttttgtcttg atacatggct cttacaaact cactaaccat tccatttaaa
     1321 ttggtgtgaa tttgcggaaa aactgtgctt tgttttacag aaattggttg tgagttttct
     1381 tcatttacta atgtatcatt aactgatttt tcgtttgctt gtcccgcgca agatgagcat
     1441 aaaagtatca gaaaaaccaa aattaaattt gttgttttga ataattgctt aaggcccatg
     1501 tttgtcatag tggtggtttt atgattgatg cgcaaccttg tgcttatttg catttttgcc
     1561 gtttgcgtca cattaatttg aaaaactaca ataagatttg aattagtaaa ataaaattaa
     1621 aacacaatca acttgtgtct attgcgccaa gttgcagcta gcgatttttt tattcactca
     1681 ttatccgcca aaatatctgt cgccgttttg ttgccaagtt gttctgctaa accgacaagc
     1741 aatccttctg ttcctcgaat gtagcagagt ttataaatgt tttgaaaatt tacaacttca
     1801 ccaacaattt caaccccttg ttgcaaaagt ctgatga
//
LOCUS       DAORKW010000675         1836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4180_length_1836_cov_5.453116, whole genome shotgun sequence.
ACCESSION   DAORKW010000675 DAORKW010000000
VERSION     DAORKW010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4180_length_1836_cov_5.453116"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLB46_16860"
     CDS             complement(<1..794)
                     /locus_tag="PLB46_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HRF78252.1"
                     /translation="MSNTSFFQSVEKNFEKAAKITGHPRGLLDQIRVCNSVYHMKFPV
                     KVKGEFQVIEAWRVQHSHHRTPTKGGIRYDSMVNEDEVMALASLMTYKCAIVDVPFGG
                     AKGGIKINPKEFSTEELERVTRRYAAELCKKQMLGPGLDVPAPDYGTGEKEMSWIVDT
                     YMALNPGSVDGYGCVTGKPVTQHGINGRREATGLGVFYGVREAVGFADDMAELGLSTG
                     LKGKKVIVQGLGNVGYYSAKFFQDGGATIVGIGEYEGAIYNANGLDV"
     gene            complement(859..1110)
                     /locus_tag="PLB46_16865"
     CDS             complement(859..1110)
                     /locus_tag="PLB46_16865"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HRF78253.1"
                     /translation="MKKAKSYIQKLISFFFLMATITFAHAQTVEADANGNVVDNVLRS
                     NGLIYVVVGVVVIILAGLIFYLTTIDRKLSKLEKEIKNK"
     gene            complement(1107..1799)
                     /gene="ccsA"
                     /locus_tag="PLB46_16870"
     CDS             complement(1107..1799)
                     /gene="ccsA"
                     /locus_tag="PLB46_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRF78254.1"
                     /translation="MLKQLHTKWWKVLCVLLLGYTFIYGLLVEIPNVALLHETVRNLF
                     FHVPCWFAMLLLMLTSLVYSIKYLRTGKTKYDLAAVALLRMAVTFGILGYLTGMLWGN
                     YIWGNLVSWITNDTKILGTAIGLLIYFAYFILRGSIEDEEKRARVSAVYSIFAFILLN
                     VAINVVPRLTDSLHPGSGGNATFTVYELDNHLRMVFYPAVIGYFLLGLWISSIIYRMG
                     RLHYLNNNIPIE"
BASE COUNT          569 a          402 c          334 g          531 t
ORIGIN      
        1 acatcaagtc cgtttgcatt ataaattgca ccttcatatt cgccgatacc aacgatagtt
       61 gcaccaccat cctggaagaa tttcgcagaa tagtaaccaa cgttaccaag accctgaaca
      121 atcacttttt taccttttaa gccggtagat aatccaagtt cagccatatc atcagcaaaa
      181 ccaacagctt cacgaacgcc ataaaataca cctaaaccgg ttgcttcacg acgaccgtta
      241 ataccgtgct gcgtaacagg tttaccggta acacaaccgt aaccgtcaac actacccgga
      301 tttaacgcca tataggtatc tacaatccag ctcatttctt tttcacctgt tccgtaatcc
      361 ggagcgggaa cgtctaagcc tggacctaac atttgttttt tgcataattc agccgcataa
      421 cgacgtgtaa cgcgctctaa ctcttcagtt gaaaattctt tagggttaat tttaatacca
      481 cctttagctc caccaaatgg aacatctaca atagcacatt tgtaagtcat taaactggct
      541 aaggccataa cttcatcttc gttaaccatt gagtcgtaac gaataccacc tttagtaggt
      601 gtgcggtgat ggctatgttg aacacgccat gcttctatca cctgaaattc accctttact
      661 tttaccggaa atttcatgtg gtaaacactg ttacacactc tgatctggtc gaggagacct
      721 ctagggtgac cagtgatctt cgctgctttt tcgaagtttt tttctacgct ttgaaagaaa
      781 ctagtatttg acatataaaa taattggttt taaaattgtt aatagtgatc gtttatttat
      841 tccgaagtcc ggattttttt acttgttttt aatttctttt tccaatttgc tcaatttgcg
      901 gtcaatggtg gtcaggtaaa aaattaaccc ggccaaaatg ataacaacta ctccaaccac
      961 tacgtaaata aggccattgc tgcgcaaaac attatctaca acattgccat tcgcatcagc
     1021 ttctaccgtt tgagcgtgcg caaaggtaat ggttgccatc aaaaaaaaga atgatattaa
     1081 tttctgaata taggatttag cttttttcat tcgataggta tattattatt gagataatga
     1141 agcctgccca tacggtaaat tatggatgaa atccacaggc caagcaggaa ataaccaatt
     1201 accgcagggt aaaacaccat acgtaaatgg ttgtcgagct catacacggt aaaagtggca
     1261 tttccgccgc ttcccgggtg caatgagtcg gttaatcttg gaactacgtt tatggctaca
     1321 ttgagtaaaa tgaaggcaaa aatgctgtaa acagcagaaa ctctagctct tttttcttca
     1381 tcttcaatac tgccgcgcaa aataaagtaa gcgaaatata tgagtaggcc gatggcagtc
     1441 cccaaaatct tggtatcgtt ggttatccag ctaaccagat tgccccaaat ataattgccc
     1501 cataacatgc ctgtcaggta tcctaaaata ccgaaggtta ctgccatacg taataaggct
     1561 acagcagcaa ggtcgtattt agttttaccg gtgcggagat atttaatact atacaccaat
     1621 gaggtgagca ttaacaataa catggcaaac cagcaaggca cgtggaaaaa gaggttgcgt
     1681 acggtttcgt gtaataaggc tacgttgggt atttcaacca gtaaaccgta gataaatgtg
     1741 tatccgagca ataacacaca aagcaccttc caccatttgg tatgcaactg tttcagcatt
     1801 ttttattgat cagtatcaca aaaaatgctg aaacag
//
LOCUS       DAORKW010000676         1830 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4204_length_1830_cov_5.572394, whole genome shotgun sequence.
ACCESSION   DAORKW010000676 DAORKW010000000
VERSION     DAORKW010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4204_length_1830_cov_5.572394"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1020)
                     /locus_tag="PLB46_16875"
     CDS             complement(130..1020)
                     /locus_tag="PLB46_16875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78255.1"
                     /translation="MKNNKHMSYFEYSLLASTFILMSRQVNGQVVYYDVIPDIYISNF
                     PGENFDLDNNGSDDFFIIAEKLSDYNSSDIICYSSLIFEVGNYNSNNHIIGLFNPYEK
                     DFCLPLTTNYHINSEAILDGDIIQYMTIKQRVVDCFSYTPEGDWHYFDLAGWQESATE
                     DKFIGVRFDSGIEECLYYGWIRCVFNESMDTLTVKDYAYNSVCNEGLFAGSLISSVQQ
                     LQAFEFNALVQQNQLFLTIPIKVLGARLTIYNSVGQDVFSKSINSETEVFDISTLAGG
                     IYCAVLTYDGKQSGKLIYKK"
BASE COUNT          620 a          325 c          307 g          578 t
ORIGIN      
        1 tatattttgt aggggcgaat tgaatccgcc catataaaat ataaatgatg acaactacgg
       61 agtgataaat taaactgatt aaaaaaaaac acagtcctgg ccgagcaagg ttcttacttc
      121 aatataaaat tatttcttat aaattaattt gccggattgt tttccatcat atgttaacac
      181 tgcacaataa atgccgcctg ctaatgtaga gatgtcaaac acctccgttt cagaatttat
      241 acttttggaa aaaacatcct gcccaacgct gttgtagata gttaatctcg cacctaaaac
      301 cttaattggg attgttaaaa aaagctgatt ttgttgaacc agcgcattaa actcaaaggc
      361 ttgtaattgt tgaacactgc taataagtga acccgcaaac aacccttcat tgcaaactga
      421 attataagca taatctttta ccgttaaagt atccattgat tcgttaaaaa cacaacgtat
      481 ccaaccatag tataaacatt cttctattcc tgaatcaaaa cggacaccaa taaatttatc
      541 ttcggtagcg ctctcctgcc agccggcaag atcaaaataa tgccaatcac cttcgggtgt
      601 ataggaaaaa caatcgacta cgcgttgttt tatggtcata tattgaataa tatcaccatc
      661 taatattgct tcactgttaa tatgatagtt tgtagtcagc ggtaagcaaa aatctttttc
      721 gtacggattg aataaaccaa ttatatgatt attcgagttg tagtttccaa cttcaaatat
      781 taaacttgaa taacatatta tatctgaaga attataatcg ctaagttttt ctgcaattat
      841 aaaaaagtcg tcacttccat tattatctaa atcgaaattt tcacctggaa aattacttat
      901 ataaatatcc ggtattacat cataatatac aacttggcca ttaacttgcc gagacataag
      961 aataaacgta ctagccaata gcgaatattc gaaatatgac atatgtttat tgttcttcat
     1021 aatttcacta aatgttttat ttgaaactat tgctaagata atcttttacg gatagtttat
     1081 tgctttgaaa agctttcatg cacttggtat aaatgcccat ttaccactcg gacataaata
     1141 taataggatt tatcctaacc caccgcaaat ggtagtggtt tgtatgggcg gatgcaattc
     1201 acccctacaa accactacca tttgcttaat tccataaatt tttaatcttg cgaaaaactg
     1261 tcagcattta tattttgtag gggcgaattg catccgccca tataaaatat aaatgatgac
     1321 aactacagag cgaaacattg ataccggcag taaaaatatt taatacaaat tatttcaatt
     1381 tacatcccag atgttcgcag ggttttactg attgtttatt ttattcaggt gaaagtaccc
     1441 tgctaggacg ttaatggtaa taaaccacaa cataaaatta catgagaacc tcatgctgta
     1501 cagtttagcc cggatccacg atagctatcg ggatggaggc caggggggga ggaaaaattt
     1561 gtgaattgaa gattgaggga tttggagatt taggattacg agccgaggat aaagcccctg
     1621 ccggaatgga tagccggttg ggaagctgat ttgaaatggg atgttaatgc tccaaaaaaa
     1681 atcagcttcc tttgcggtta tatgcgatta catttctgca taatatttaa aaaaccatgg
     1741 caatgtttcg atgcatttgt aataattaaa caagcggtat ttttcgtttg gtgaatggat
     1801 attatcgctg tttaatccga agcccattaa
//
LOCUS       DAORKW010000677         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4225_length_1825_cov_4.566102, whole genome shotgun sequence.
ACCESSION   DAORKW010000677 DAORKW010000000
VERSION     DAORKW010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4225_length_1825_cov_4.566102"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..483
                     /locus_tag="PLB46_16880"
     CDS             55..483
                     /locus_tag="PLB46_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRF78256.1"
                     /translation="MIFKRNASDFMTANVIVAGENNSFEQVMTFFTVHKIQHLPVAEN
                     NKLIGIVSMKDMLSFIARQLNDNPMISKEMLNNNFNIKEVMTAEPVTVEPESTQKEIL
                     EILAAGKFQAVPVVKGGIIKGIITNKDITRIYHYDATHLI"
     gene            complement(637..1812)
                     /gene="nhaA"
                     /locus_tag="PLB46_16885"
     CDS             complement(637..1812)
                     /gene="nhaA"
                     /locus_tag="PLB46_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRF78257.1"
                     /translation="MRITKLFSEFVKSESSGGLILLFCTLISLIIVNSPIGAQYEHLW
                     HIKIGQLSLSHWINDLLMAVFFLLVGLEIEREIYVGELKSPGKAIFPIIAALGGMLIP
                     AGLYLGININAENHDGFGIPMATDIAFALGMLSLLGSRIPLGVKIFITALAIIDDLGA
                     IIIIAIFYTEELKLDYLSYAGIFYAVLLVLNRLNVKYVYPYLILGLVMWFFMLQSGVH
                     ATLTGVLLAFAIPFRDGGTKSPSYIMQHTLHKPVAFFILPLFALANTAILINPTVLNG
                     FIQSHTLGIMAGLLLGKPIGILLFTYLAVKLKIAALPEGVNWNLIIGASILAGIGFTM
                     SIFISMLAYSDEYIINISKLSVLIASAIAAIIGLIWLYTFTKKPLAGEEHTEEVEMP"
BASE COUNT          627 a          335 c          334 g          529 t
ORIGIN      
        1 tcatacttcc caacgatgac ctgtgatagt tttgtggtat aaaacaacta gtatatgata
       61 tttaaacgca acgcttctga ttttatgact gccaacgtga ttgttgcagg tgaaaacaat
      121 tcatttgagc aggtaatgac cttttttacc gtgcataaaa ttcagcactt acctgtggct
      181 gaaaacaata agctgatagg tatagtgagt atgaaagata tgttgagttt tattgcacgt
      241 caactcaacg acaaccctat gattagcaag gagatgttaa ataacaattt taacattaaa
      301 gaggttatga cggctgagcc tgtaacagtt gagcctgaat cgactcaaaa agagattttg
      361 gaaattttag cagctggcaa atttcaggca gttcctgtgg tgaaaggtgg aattataaag
      421 ggaattatta cgaataagga tatcaccaga atttaccact acgatgccac ccatctaatt
      481 taaaaagcgg taaaatattt gtcattttat tccttttttt tggaaaaaaa gtttcaaaaa
      541 aattgggaaa attcaaaaag gcctatatat ttgtactgtt tttaagggat aaacaagcag
      601 caagcgaccc gaaagggtca attgctgttt ttctttttat ggcatttcca cttcttctgt
      661 gtgctcctct cctgccaacg gttttttcgt aaaagtatat aaccagataa ggccaattat
      721 agctgcaatt gcagacgcaa taagcactga taatttagaa atatttataa tatattcgtc
      781 actataggcc aacatactaa taaagatgct catggtaaaa ccaatacctg ctaaaatgga
      841 agctccaata atcaggttcc aattgacccc ttcgggcaag gcagcaattt taagttttac
      901 ggctaagtag gtaaaaagca ggattccgat aggttttccc aataataaac ctgccataat
      961 acccaacgta tgtgattgga taaagccatt aagcaccgtt gggttgatta aaatggcggt
     1021 gttcgctaat gcaaataagg gtaaaataaa aaacgccaca ggcttgtgaa gcgtatgttg
     1081 catgatataa ctaggtgatt tagtaccacc gtcacgaaat ggaatggcaa aagcaagcaa
     1141 tactccggtt aaggtagcat gcacaccgct ttgcagcata aaaaaccaca ttactaaccc
     1201 gagtattaaa taaggataaa catatttaac attcaaacgg tttagcacca ataaaacagc
     1261 ataaaaaata ccggcataac tgaggtaatc taactttaac tcctctgtat aaaaaatggc
     1321 gattatgata attgcgccta aatcgtcgat tatggccagg gctgtgataa atatttttac
     1381 acctagtgga attcggcttc ctaatagtga tagcatgcct aaagcaaatg caatatcagt
     1441 agccatggga atgccaaaac catcatggtt ttcggcattg atatttattc ccaaatataa
     1501 tccggcaggt ataagcatgc ctcccagtgc tgcaattatt ggaaaaatag ctttccccgg
     1561 tgattttaat tcacccacat aaatttcccg ttcaatttcg aggcctacca ataaaaagaa
     1621 aacggccatc aataaatcat ttatccaatg ggataaactc agttgtccga ttttaatatg
     1681 ccacaaatgt tcatactggg ctcctatcgg actattcaca ataattaaag aaataagtgt
     1741 acagaatagc agaattaagc cgcctgaact ttcgcttttt acaaattcgc taaacagttt
     1801 tgtgattcgc attggataaa attat
//
LOCUS       DAORKW010000678         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4226_length_1825_cov_3.288701, whole genome shotgun sequence.
ACCESSION   DAORKW010000678 DAORKW010000000
VERSION     DAORKW010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4226_length_1825_cov_3.288701"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLB46_16890"
     CDS             <1..364
                     /locus_tag="PLB46_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HRF78258.1"
                     /translation="MNVAQMLAHCNVTYEMIYTDKHPKPNFFMGLVLKMFVKKIVTNE
                     APYKHNSPTAPAFLIVDERVFATEKERLINHIIKTQETGAAAFDGKVSNSFGKLNVTE
                     WNNMMYKHLNHHLTQFGV"
     gene            433..1350
                     /locus_tag="PLB46_16895"
     CDS             433..1350
                     /locus_tag="PLB46_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78259.1"
                     /translation="MFANSSVYFDETSQKLDAINVLSNTTDSTTQNIQDTAVRKTNTL
                     KDDIYLHPIDCITPYTLQKGEWIYGQSWQTLPFPSWAFYGITNNLTMQIDLLPWLYGA
                     FTELKKPIPSVNFRYKFTDQNGALPTIGAEAMFIYLWDTLLRFETPSISVQESGAYFH
                     FKPSIGYRIKNDWYINLSAGVDYMKSLILLNNDTTLNTYKDFTNTWNPNYAIGIDYRP
                     SNWISYHLAYQYGATFTYLENVPRKTMLTYGFRFAPFYKNKYGILRNMRFELVSINGY
                     FADVDAWQAIGLPIYPYVYWQWKRDKTKK"
     gene            1496..1642
                     /locus_tag="PLB46_16900"
     CDS             1496..1642
                     /locus_tag="PLB46_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78260.1"
                     /translation="MNVHSGLSGAFAFYCPEKDCYITGTVNQIHKPGSSFKLMVKLLQ
                     QVPE"
BASE COUNT          611 a          317 c          301 g          596 t
ORIGIN      
        1 aatgaatgtg gctcaaatgt tagcccattg taatgtgact tacgaaatga tttatacaga
       61 caaacatccc aaacctaatt tttttatggg actagtatta aaaatgtttg tcaaaaaaat
      121 tgttactaac gaagcaccct ataaacacaa ctccccaact gctccggctt ttttaattgt
      181 agatgaaaga gtatttgcca ccgaaaaaga gcgtctcatt aatcatatta tcaagacaca
      241 ggaaacaggt gcagctgcat ttgacggcaa agtttccaat tcttttggca aactcaatgt
      301 taccgaatgg aataatatga tgtataaaca cttaaatcat cacttaacgc aatttggcgt
      361 gtaacgatgt ttaaattatt ggcaagcata ggtattacta tgtgcgcaat tttatgttgt
      421 tgcaccatac caatgtttgc caattcatca gtttattttg acgaaacaag tcagaagtta
      481 gatgcgataa atgtattgtc aaatactact gacagcacaa cacaaaacat tcaagatact
      541 gctgtcagga aaacgaatac acttaaagat gatatttact tacatcccat agattgtata
      601 actccatata ccttacaaaa aggagaatgg atatacggac aatcatggca aacactgcca
      661 ttcccaagtt gggcatttta tggtattacc aataatttaa ccatgcaaat tgatttgttg
      721 ccatggttgt atggtgcatt taccgaatta aaaaagccaa ttcccagtgt taactttcga
      781 tataaattta ccgaccaaaa tggagctttg cctaccatag gcgcagaagc catgtttatt
      841 tatttatggg atactttgtt acgttttgaa acaccttcta tttcggttca ggaaagcggt
      901 gcgtattttc attttaaacc aagtattggt tatcggataa aaaatgattg gtatatcaat
      961 ttatcagcag gtgttgatta tatgaaaagt ttaattctac taaacaatga caccacacta
     1021 aatacataca aagatttcac caatacctgg aaccccaatt atgcaattgg aattgattac
     1081 agaccatcta attggattag ttatcatctc gcttatcaat atggtgccac atttacctat
     1141 ttagaaaatg ttcctcgcaa aacgatgcta acctacggat tcaggtttgc accattttat
     1201 aaaaataaat acggcatctt acgtaatatg cgcttcgaat tagtatctat taatggctat
     1261 tttgcagatg ttgatgcctg gcaggcaata ggtttaccta tttatccata cgtgtattgg
     1321 caatggaaaa gggataaaac gaaaaaataa attagttata attaatacca caggaacctc
     1381 cattattttg taatgtattt tttatttaat ttatacttac atgtcattac tttttagtcg
     1441 aattacaagt acaaaatgat aaggttattt ttacactagg tgccctaatc accaaatgaa
     1501 tgtacattca gggttatcag gagcatttgc attttattgc cccgaaaaag attgttatat
     1561 cacaggaacg gtaaatcaaa ttcataaacc gggcagttca tttaaactca tggtgaaatt
     1621 attacagcaa gtgcctgaat aataattccg accatttatc ttatctctgt ttaaactgca
     1681 gcagtgttta acaaaaacta cgtttctaaa aaccaatttc caattctgct gttcgtgtta
     1741 taaatttacc gcatgcagtt attgatggat ctggggtttt tggagcttaa ttttgttgat
     1801 gtgattgaca ttttgttggt cgcat
//
LOCUS       DAORKW010000679         1824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4228_length_1824_cov_4.991521, whole genome shotgun sequence.
ACCESSION   DAORKW010000679 DAORKW010000000
VERSION     DAORKW010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4228_length_1824_cov_4.991521"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLB46_16905"
     CDS             <1..918
                     /locus_tag="PLB46_16905"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HRF78261.1"
                     /translation="SLNAVDLGDFIVKMSHDIGFKADILNKSQIEALRMGGLLGVNAG
                     SSVPPTFSILEYKPENAKNVKPLIFVGKGVTYDTGGYSIKTGPGMPGMKMDMSGAAAV
                     VGTFYAIVKNKLPVYCIGLIPATDNRINSNALVPDDIITMHSGTTVEVLNTDAEGRLI
                     LGDALSYAKKFQPELVIDLATLTGAAAAITGSLGGALMGTDTKYRPTLMACGNETYER
                     LWEIPYWREYADMLKSDIADLKNIGGAVGGSATAGKFLEHFTDYPWLHLDIAGPAILK
                     DDEPYKLKGASGVGVRLLYAFTKKFAELS"
     gene            complement(928..1518)
                     /locus_tag="PLB46_16910"
     CDS             complement(928..1518)
                     /locus_tag="PLB46_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF78262.1"
                     /translation="MKKTLFLITMLLPIALLAQRYPTGKGTFRTGGGGSYSQSDKGDY
                     NLYKLTITPRLGYFVTDYLSVGFQMNYSISYDTNYTSALKFTPQVKAYYPINDHWFII
                     GNFEVGVDRTTVFADNKTITDHTSISFGPGVSYFYNRRIGFELNPLYQIYINPEDNYT
                     NRFYTEGGVVFNILNKSERNKANRKKNVEYDELEDE"
     gene            complement(1559..>1824)
                     /locus_tag="PLB46_16915"
     CDS             complement(1559..>1824)
                     /locus_tag="PLB46_16915"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78263.1"
                     /translation="DLMAIKIFIDADADHRLMRVVKRDMEERGRDIDQVMTRYLNTVR
                     PMHEQFIEPSKRFADIIVPVGGDNHVAIDLLSQYIAAQLSNSA"
BASE COUNT          537 a          353 c          387 g          547 t
ORIGIN      
        1 tcactaaatg ccgttgacct tggtgatttt atcgtaaaaa tgagtcacga tattggattt
       61 aaagctgata ttttaaataa aagtcagatt gaagctttac gtatgggtgg cttgttaggc
      121 gtaaatgcag gaagctcagt acctcctact ttttccattt tggagtacaa gcccgaaaat
      181 gctaaaaacg ttaagccatt gatatttgta ggcaaaggcg taacctacga tacaggagga
      241 tacagcatca aaaccggacc tggaatgccg ggaatgaaaa tggatatgtc gggtgcggct
      301 gcagttgtgg gaacatttta tgcaatcgta aaaaacaaac tccctgtata ttgtatcggg
      361 ttaattcctg ctacagataa caggataaat tcaaatgctt tagttcctga tgatattatt
      421 accatgcaca gtggaactac agtagaagtg ctcaacacag atgctgaagg acgtttaata
      481 ttaggtgatg cgttgagtta cgcgaaaaaa tttcaacctg aattggttat cgacctggct
      541 actctaactg gtgctgctgc tgcgataacc ggctctttag gtggtgcatt gatgggcaca
      601 gataccaaat atcgacctac ccttatggca tgtggcaacg aaacttatga gcgcttatgg
      661 gaaatcccat actggcgcga atatgccgac atgctaaaaa gtgatatcgc cgatttaaaa
      721 aacattggtg gtgccgtagg cggaagtgct acagcaggca agttcctcga acattttact
      781 gattatcctt ggttacatct cgatattgca ggtccagcca tcttaaagga tgatgagcct
      841 tacaaattga aaggtgctag cggcgttgga gtgcggttat tatacgcttt caccaaaaaa
      901 tttgcagaac tcagttaatc agcatattta ttcatcttcc aattcatcgt attcgacatt
      961 ttttttgcga ttagctttgt tgcgctcgct tttgtttaaa atgttaaata caactccacc
     1021 ttctgtgtaa aaacggttgg tataattgtc ttctgggttg atataaatct gatataaggg
     1081 atttaattca aaccctattc tcctgttata aaagtaggat acaccgggac caaatgaaat
     1141 actggtatgg tcggtaatag tcttattatc agcaaaaacc gtggttctgt caacaccaac
     1201 ttcaaaattg ccaatgataa accaatggtc gtttattgga tagtaggcct ttacctgggg
     1261 cgtaaatttt aatgcagatg tgtagttggt gtcatacgaa atggaatagt tcatttgaaa
     1321 ccccaccgac aaataatctg tcacaaaata gcccagtctc ggtgtaatgg taagcttata
     1381 caagttataa tcgcccttgt cagactgact gtaagaacca cctccccctg tccggaaagt
     1441 cccctttcct gttggatagc gctgtgccaa tagggcgatt ggcagcagca tggtgattaa
     1501 aaatagtgtt ttcttcatgg tttgggtatt tttaaatcga gtataaatat attcagattt
     1561 atgctgaatt tgataactga gcagcaatat attggcttag aagatcaatg gccacatgat
     1621 tatcgccgcc tacaggaaca ataatatcgg caaacctttt tgaaggttca ataaattgtt
     1681 catgcatagg gcgcaccgta tttaagtatc tggtcatcac ctggtcgata tctctgccgc
     1741 gctcttccat atcgcgtttt actacacgca ttaatcggtg atcagcgtct gcatcgataa
     1801 atatcttgat agccatcaaa tccc
//
LOCUS       DAORKW010000680         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4265_length_1818_cov_2.964833, whole genome shotgun sequence.
ACCESSION   DAORKW010000680 DAORKW010000000
VERSION     DAORKW010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4265_length_1818_cov_2.964833"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="PLB46_16920"
     CDS             complement(<1..1437)
                     /locus_tag="PLB46_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78264.1"
                     /translation="MGRIVVSQNARQRQNDQYSFTRYDNLGRIYQTGQLEQATEMTFA
                     IALDNEDLFDWIDVGTNKREISSTFYDNEALPVGVHFTNSEQTNLRNRISSVTWETED
                     DEDSLTYDYATHYSYDVSGNVFTLIQELGGLRGTGNDFKRMDYEYDLISGKVNQVSYQ
                     PDSADQFYHQYIYDEDNRLVNALTSVDEHLWQNEATYLYYKHGPLRRTEIGERQVQGM
                     DFAYTLQGWLKGMNSEGRDQGKDMGSDGKTSGSHKLFGRDVASFLLSYFEGDYRPIGT
                     NRFTATYSTSGGFGASSPSLYNGNIRKATYSLANLTDPVVGYAYGYDQLNRLISMNAW
                     FNFNTGSYAWQTSGSASNKLQERVSYDANGNITKYLRKGYSPINMDSLTYKYYPKSNK
                     LLRVDDSVSVSTYADIDVDDQDSLNYEYDLTGNLVKDSIEGISLITWNLMGKVDSIAR
                     YSASSRPELKFLYDAMGNRVGKVVYDGAD"
BASE COUNT          522 a          381 c          290 g          625 t
ORIGIN      
        1 atcggcgcca tcgtaaacca ctttccctac ccgattaccc attgcatcat acaaaaactt
       61 aagttccggt ctactgcttg cactatatcg agctatacta tctactttac ccatgagatt
      121 ccaagtaata agacttattc cttctataga gtcttttact aaattacctg taaggtcata
      181 ctcataatta agactatcct gatcatcaac atcgatatca gcataagtac tcacacttac
      241 tgaatcatct acccgtaaca atttattaga ctttggataa tatttataag taaggctatc
      301 catattgatg gggctatacc cttttcgtaa atattttgtg atattaccat tggcatcgta
      361 tgaaactctt tcctgtaatt tatttgaagc acttccactt gtttgccatg cataggaacc
      421 ggtattaaaa ttgaaccagg cattcatact aattagtcta tttaattggt cgtaaccata
      481 ggcataacct actacagggt ctgttaaatt agctaaactg taagttgctt ttcgaatatt
      541 accattatat aaagatggac tacttgcacc aaaccccccg gatgtggaat atgttgctgt
      601 aaatctattt gtgccaatag gacgataatc accttcaaag taactcaata ggaaactggc
      661 tacatcacgt ccaaatagct tgtgagaacc acttgtttta ccatcactac ccatgtcttt
      721 tccctggtct cttccttcac tattcatacc ttttaaccaa ccttgtaaag tgtacgcaaa
      781 atccattccc tgtacctgtc gctcacctat ttcagttctt cttagcggac cgtgtttgta
      841 atacaaatag gttgcttcat tttgccataa atgttcgtct acacttgtaa gtgcattaac
      901 taatctattg tcttcatcat aaatatattg atgataaaac tggtcagcag aatcaggttg
      961 ataacttact tgatttactt ttccactgat caagtcatat tcatagtcca tacgtttaaa
     1021 gtcgttaccg gtgccacgta aaccacccag ttcctggata agcgtaaata cattacccga
     1081 cacatcgtaa ctataatgtg ttgcataatc gtaggttaat gaatcttcat catcttcggt
     1141 ttcccaagtt acacttgaaa tgcggttcct taaattagtt tgttcactat tggtaaaatg
     1201 cacacctacc ggcaatgcct cgttgtcgta aaacgtactg gagatttcac gtttgttcgt
     1261 acctacatca atccaatcaa ataaatcttc attatcaagt gcaattgcaa atgtcatttc
     1321 tgttgcttgc tccaattgcc cggtttgata aatacgacca aggttatcat atcgagtaaa
     1381 tgaatattgg tcattttgtc gttgacgtgc gttttgagaa actacaattc tacccaaacg
     1441 gtcataccaa aatctagtgg tgtcaccatc aggtgtatat tgtttaatga gttggtttaa
     1501 agtgttataa taatatcggg tttttaattt atgatcagga tattccggac ttcctgagcc
     1561 atcaagtcga taatcggctt gatttgtctg ctcagttgca tctaaaaagt caacaccttc
     1621 aggtggtact aataattatc cctgccattt atctcatctc tgtttaaact ggagcagtgt
     1681 ttaacattaa ctgcgtttct aaaaaccaat ttccaattct gctattcgtg ttataaattt
     1741 accgcatgca gttattgatg gacctggggt ttttggagct taattttgtt gatgtgattg
     1801 acattttgtt ggtcgcat
//
LOCUS       DAORKW010000681         1813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4285_length_1813_cov_5.025028, whole genome shotgun sequence.
ACCESSION   DAORKW010000681 DAORKW010000000
VERSION     DAORKW010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4285_length_1813_cov_5.025028"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..>1813)
                     /locus_tag="PLB46_16925"
     CDS             complement(1..>1813)
                     /locus_tag="PLB46_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRF78265.1"
                     /translation="ISALKDNEDIVEPLSERIIGRTSLHNVYDPLNDELIVHAGELIT
                     ERIANRIDETAIEAVEIRSVLTCESKKGVCVKCYGTNLSNNRVAQAGDAVGIIAAQSI
                     GEPGTQLTLRTFHVGGVAGSVDTESKLVAKFDGKVEFDGVRFVDTKNDEGKKVRIVIG
                     RTGEVKLIEAKTNKLLITNNVPYGSLLAVSDGDELKKGEAICSWDPFNAVIVSEFPGI
                     VEYEHIIEGITFREESDEQTGHREKVISESRDKTKIPTILIKDKKTGDVKSYNLPVGA
                     HINVEEGDEVKSGQTIAKIPRALGKVRDITGGLPRVTELFEARNPSNPAVVAEIDGVV
                     AFGKIKRGNREIEIESRDGEKKKYLVPLSKQILVQENDYVYAGMPLSDGAITPSDILA
                     IKGPYAVQEYIVNEIQEVYRLQGVKINDKHIEVIVRQMMRKMTITDAGDTRFLEDANV
                     DKLDFLEENDRIFDKKVITEAGDSTKYKAGHIVTLRQLREENSHLKRADKKPIEYRDA
                     VSATATPLLLGITRASLGTQSWISAASFQETTKVLSTASISAKTDYLMGLKENVIVGH
                     QIPAGTGQRHFLNLLVGNKEEVEELAAAKKKKSIELE"
BASE COUNT          482 a          391 c          327 g          613 t
ORIGIN      
        1 ttattccagt tcaattgatt tcttcttttt agctgctgct aattcttcaa cttcttcttt
       61 gttaccaacc agtaaattga ggaagtggcg ctgacctgta cctgcaggga tttgatgtcc
      121 aacaattacg ttttctttca gacccatcag ataatctgtt tttgcagata tcgacgctgt
      181 gctcaatact ttggttgttt cctggaatga tgctgcagaa atccagcttt gtgttcctaa
      241 tgaagctctg gtaataccaa gcaacaatgg agtagctgta gcagaaactg catcgcgata
      301 ttcgataggt tttttatcag cgcgtttcaa gtgtgaattt tcttcacgca actgacgtaa
      361 agtaacaata tgtccggctt tatatttagt tgaatcaccg gcttcagtaa ttactttttt
      421 atcgaatatt ctatcatttt cttcaaggaa atcgagttta tctacgttcg catcttcgag
      481 gaaacgagta tcacctgcat cggtaatcgt catttttctc atcatctgac gaacaataac
      541 ttcgatatgt ttatcattga tttttacccc ttgtaaacga taaacttcct gaatttcatt
      601 tacgatatat tcctgaacgg cataaggacc tttaatagca agaatgtcgc ttggtgtaat
      661 agcaccatca ctcaacggca taccggcgta tacatagtca ttttcctgaa caagaatttg
      721 ttttgaaagt ggaacgaggt attttttctt ttcaccatcg cggctttcaa tttcaatttc
      781 gcggttacca cgtttaattt taccgaaggc aacaacacca tcaatttctg caacaactgc
      841 agggtttgaa ggattacgcg cttcaaataa ttcggttaca cgtggaagac cacccgtgat
      901 atctcttact ttacctaatg cacgaggtat tttcgcaata gtttgacctg attttacttc
      961 atcaccttct tcaacgttga tgtgagcgcc tacaggtaag ttatagcttt ttacatcgcc
     1021 ggttttctta tccttaataa ggatagtagg tattttagtt ttatcgcgtg attctgaaat
     1081 tactttttcg cggtgaccgg tttgttcgtc gctttcttca cggaaggtga taccttcaat
     1141 gatatgttcg tattcaacaa taccagggaa ttcagaaacg attacggcgt taaatggatc
     1201 ccaagaacaa attgcttcac cttttttcaa ttcatcgcca tcgcttacag ctaataatga
     1261 accgtaagga acgttgttgg tgattaacaa tttatttgtt ttcgcttcta tgagtttaac
     1321 ctcaccggta cgaccaatta cgatacgtac ttttttgcct tcatcatttt tagtatcaac
     1381 aaaacgaaca ccgtcaaatt caactttacc gtcaaatttc gcaacgagtt tcgattctgt
     1441 atcaacgctt cccgcaacac cacccacgtg gaatgtacga agtgtaagct gtgttcccgg
     1501 ttcaccgatt gactgtgcgg caataatacc tacagcgtca ccggcttgtg ctacgcggtt
     1561 gttagaaagg ttagttccat aacatttaac acaaacacct tttttcgatt cgcatgttaa
     1621 cacggaacga atttccactg cttcaatcgc agtttcgtcg atgcggttag caattctttc
     1681 tgtaattaat tcacctgcgt gaacaattaa ttcgtcgtta agcgggtcgt aaacattgtg
     1741 caaacttgtg cggccaatga tacgttcact taatggttca acgatatctt cgttatcctt
     1801 taacgcactg ata
//
LOCUS       DAORKW010000682         1797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4360_length_1797_cov_4.780138, whole genome shotgun sequence.
ACCESSION   DAORKW010000682 DAORKW010000000
VERSION     DAORKW010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4360_length_1797_cov_4.780138"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1794
                     /locus_tag="PLB46_16930"
     CDS             <1..1794
                     /locus_tag="PLB46_16930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type B sorting domain-containing protein"
                     /protein_id="HRF78266.1"
                     /translation="YIYAAGLYTTTWEGEIASENLSITQITTLPEDALYFVTRVLLCN
                     DGPTAINEIYYKRNVDPDNEAAWIGEGFVTENTIVYQPPVDEDALVSAVSSPMGCYLG
                     MGARDLNSRVSFGSFVTEGGTPENAWNGTGPYSNEGTITEDGATQIAFYIPTILPGEC
                     KCIAFAYILNEDDLAEALDATVTYNVTAGGDIIASGDSSAVCNSGDTISLEVIGANDY
                     AWTWSPSTGLNVDTGIAVIATVTETTEYTITGVGGFCGDAVLTVTVFVDSLENLVDAG
                     ADYSICLGDETTFESEAGPIAESILWTPDLYLDCTDCEDPTLTPTEAGEYTYTLNAVT
                     KWGCPGSDDITLTVNPLPVVDAGDDTNVCPEGSVPLEATGAVEYEWTPSTGLSCDDCP
                     DPDCTVNDFTVYTVTGTDANGCVNTDEVEVSVFSELEIFASADPETIDIYLGQTSQLD
                     VTGAETYSWAPSSGLSATNIANPIAAPNDTMTYVVTGIDANGCIATDTVTVYVIGELA
                     VGIPTAFSPNGDGFNDNWAPAVTGSGYVESYMIYNRYGELVYQGSSATPGWNGIYNGE
                     LQGIGSYVVNIKAVTSLGEQRDLKGSFTLVR"
BASE COUNT          512 a          329 c          407 g          549 t
ORIGIN      
        1 tatatctacg ccgcaggcct ttatactacg acgtgggagg gagaaatagc aagtgaaaac
       61 ttatctataa cacaaatcac tacattacct gaagatgctc tttattttgt aacccgtgta
      121 ttgctttgta atgatggacc tacagcaata aatgaaattt attataaacg caacgtggat
      181 cctgataatg aagctgcttg gattggcgaa ggcttcgtaa ctgaaaatac cattgtttat
      241 caaccacctg tagacgagga tgcattggtt tctgcagtaa gctcgccaat gggttgttat
      301 ttaggtatgg gggcacgaga tttgaattca agagttagct ttggctcatt tgtcactgaa
      361 ggaggcactc ctgaaaatgc ctggaacgga acaggtccat actcaaatga gggtacgatt
      421 accgaagatg gtgccactca aattgctttt tatattccta ccattttacc cggtgaatgt
      481 aaatgtattg cattcgctta tattttaaat gaagatgatt tggcagaagc tttagatgct
      541 actgttacct ataatgttac tgccggtggc gatattattg catcgggtga ctcatctgca
      601 gtttgtaact ccggtgatac aatttcctta gaagtaattg gtgctaatga ttatgcctgg
      661 acttggtctc cgtcaaccgg tttaaatgtg gataccggta ttgctgttat tgccactgtt
      721 accgaaacta ctgaatatac cattacaggt gtaggcggtt tttgtggtga cgcggtttta
      781 accgtaaccg tttttgttga ctcattagaa aatttagttg atgcaggtgc cgattattct
      841 atttgtttag gcgatgaaac tacttttgaa tccgaggcag gcccaattgc cgaatcgata
      901 ctctggacac ctgatttata cttggattgc accgattgtg aagaccctac cttaacacca
      961 acagaagcag gagaatatac ctatacatta aatgctgtaa ctaaatgggg ctgtccaggt
     1021 tcagatgata taacacttac cgtaaatcca ttacctgtag ttgatgcggg agatgatacg
     1081 aatgtttgtc ctgaaggttc tgtgccactg gaagctaccg gtgccgtaga atatgaatgg
     1141 acaccatcaa caggattgag ttgcgacgat tgtcctgatc ctgattgtac ggttaacgat
     1201 tttaccgtgt atacagtaac gggtactgat gctaatggtt gtgtaaacac cgatgaagtt
     1261 gaagtttctg tattttcaga attagagatt tttgcatcag ccgaccctga aacaattgat
     1321 atttatttag gccaaacatc acaattggat gtaaccggtg ccgaaacata ttcatgggct
     1381 ccttcaagtg gattaagtgc aactaatatt gccaatccga tagcagcgcc aaatgatacc
     1441 atgacttacg tagtaacagg tattgatgca aatggctgta ttgctaccga tacggtaact
     1501 gtgtatgtaa ttggcgaatt ggcagtaggt ataccaaccg cattttcacc aaatggtgat
     1561 ggctttaacg acaattgggc acctgcagtt accggttccg gatatgttga aagttatatg
     1621 atttacaacc gttatggcga acttgtttac caaggttcaa gcgctactcc gggttggaat
     1681 ggtatttaca atggtgaatt gcagggcata ggttcttatg tggtaaacat aaaagcagtt
     1741 acatcgttag gtgaacaacg cgatttaaaa ggaagcttta cattagttag ataattt
//
LOCUS       DAORKW010000683         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4400_length_1787_cov_4.864319, whole genome shotgun sequence.
ACCESSION   DAORKW010000683 DAORKW010000000
VERSION     DAORKW010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4400_length_1787_cov_4.864319"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1787
                     /gene="porU"
                     /locus_tag="PLB46_16935"
     CDS             <1..>1787
                     /gene="porU"
                     /locus_tag="PLB46_16935"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HRF78267.1"
                     /translation="LFGDASFDYRYLAFDEEKNTLKIPNYESVESLSRGVSYGTDDFF
                     GFLDPTEGDNIEDTAEKLDIGVGRLPVMTAEEATQMVDKIIHYKSDESLGSWRNGLCF
                     LADDEDYNQHMKDADLLATTLKNDYPVYNIDKIYFDAYQQIPGAGGERYPEVETSINT
                     KMYSGSFIMNYLGHGNEQNWAQERVLSVDDINSWENYDKLPLFITATCSFSRYDNPDL
                     RSAGELVLLNPTGGGIALVTTVRLVISSGNYDLNKNLLNQLFVKTDGQYPALGDAVKK
                     GKNAVTTGATNNRKFILLGDPALVLNYPKYNVQTKTVNGYDVNVYVDTLKALSKVTIT
                     GEVTDESGVKLSDFNGTVYPAVYDKPVTINTLINDPTASGVGGWGPSQEFDFELQKNA
                     LYKGKASVVNGDFAFTFIVPKDISYNYGSGKLSYYAENGEEDANGYSASVIIGGTADN
                     APEDLDGPEVNVFMNDETFVFGGLTDENPVLLIKLNDNSGINALGNAVGHDITATLDE
                     DQQTLIKLNDYYESDLDEYQSGVVAYPLSGIAEGRHTVAVKAWDVYNNSGDGYTEFVV
                     AETADLALSHVLNYPNPFTTNTTFWFEHN"
BASE COUNT          584 a          281 c          385 g          537 t
ORIGIN      
        1 tattatttgg tgatgcttca tttgattacc gctacttagc ttttgatgaa gaaaaaaaca
       61 cattgaaaat tcctaattac gaaagtgttg aatcattatc gcgaggtgtg tcatatggca
      121 ctgatgattt ttttggcttc cttgacccaa cagaaggtga caatattgaa gatactgccg
      181 aaaaattaga tataggcgtg ggaagattac cggtaatgac agcagaagag gctacacaaa
      241 tggtagataa aattatccat tataaatcag atgaaagttt aggcagttgg cgaaatggat
      301 tgtgtttttt agcagatgat gaggattata atcagcatat gaaagatgcc gatttattag
      361 caactacatt aaaaaacgat tatcctgttt acaatattga taaaatatat tttgatgctt
      421 accaacaaat tccgggtgca ggcggagagc gttatccgga agttgaaacc agcattaata
      481 caaaaatgta ttccggtagt tttattatga attaccttgg ccatggcaac gaacaaaact
      541 gggcgcagga acgagtatta tctgtagatg atattaatag ctgggaaaat tatgataaac
      601 ttccattgtt tattacagca acatgttcat tttcgaggta cgacaatccc gatttgcgct
      661 ctgccggtga actcgtttta ttaaatccta caggtggtgg gattgcattg gtaacaaccg
      721 tgcgtttggt aatttcatca ggtaattatg atttaaataa aaatttatta aatcaattat
      781 ttgttaaaac cgatggccaa tatcctgccc taggcgatgc cgttaaaaaa ggtaaaaatg
      841 cagtaacaac cggtgctact aataatcgta aatttatttt attgggtgac cctgcattgg
      901 ttttaaatta tccgaaatat aatgtgcaga caaaaacggt aaatggttac gatgtaaatg
      961 tgtatgttga tacactgaag gcattaagca aagtgactat aaccggtgaa gtgactgatg
     1021 aaagcggcgt taaattatca gattttaatg gcactgttta ccctgcagtt tatgataaac
     1081 cggtaacaat taatacctta attaatgacc cgactgcgag tggtgttgga ggttgggggc
     1141 caagtcagga atttgatttc gaattacaaa aaaatgcact ttataaaggt aaagccagtg
     1201 tagtgaatgg cgattttgca tttacattta ttgttccaaa agatatttct tacaattatg
     1261 gttcaggtaa attgagttat tatgctgaaa atggtgaaga agatgccaat gggtatagtg
     1321 caagtgtaat aattggcgga actgccgata atgcacctga agatttagac ggccctgaag
     1381 tgaatgtgtt tatgaatgat gaaacttttg tttttggtgg actaactgat gaaaatccgg
     1441 tattgctgat aaaactcaac gataattccg gtatcaatgc tttaggtaat gccgttggtc
     1501 acgatataac agcaacgttg gatgaagacc agcaaaccct gataaaactc aatgattatt
     1561 acgaatcgga cctcgatgaa taccaaagtg gtgtggtagc ttatcctttg agtggcatag
     1621 cagagggaag acatacagtt gcggttaaag cctgggatgt ttacaataac tccggcgacg
     1681 ggtatactga atttgtggta gcagaaaccg cagaccttgc cttgagccat gttttaaact
     1741 accccaatcc atttacaacc aacaccactt tttggtttga acataac
//
LOCUS       DAORKW010000684         1784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4421_length_1784_cov_4.725275, whole genome shotgun sequence.
ACCESSION   DAORKW010000684 DAORKW010000000
VERSION     DAORKW010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4421_length_1784_cov_4.725275"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /gene="ggt"
                     /locus_tag="PLB46_16940"
     CDS             <1..1307
                     /gene="ggt"
                     /locus_tag="PLB46_16940"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRF78268.1"
                     /translation="LASGVPGSVAGMWEAHQKYGSMDWKKLLQPAIELAQKGFPITAH
                     QADDFNQLQSELKQLNKGKNYFIKSKWNANDTLIQTDLATTLIAIRDKGRDGFYTGPV
                     AEKIAAEMQQHNGIISTDDLKNYQAVWRDPIKGSYKNYTIISMPPPSSGGIALMQLLN
                     MMENYPLKKWGFHSVNSVSIMTEAEKRVYADRATWLGDPDFTKVPKNELISKTYAASR
                     MQNVDTNTITPAAQIKAGTFTGYESEETTHFSIVDKYGNAVSITTTLNDSYGSRIIVN
                     GCGFILNNEMDDFSAKPGEPNLYGLIGGEANAIAPGKRMLSSMTPTIVEKEGKLFMVV
                     GTPGGSTIITSVFQNIINVIEFDMTMQEAVDAPRFHNQWLPDEIKVENDFNETTVAAL
                     KAKGYKIIGREAIGRVDAILVYPNGKLEGAADKRGDDVAVGW"
     gene            complement(1309..>1784)
                     /locus_tag="PLB46_16945"
     CDS             complement(1309..>1784)
                     /locus_tag="PLB46_16945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78269.1"
                     /translation="VAIPTSTDPIQDYELDVIELVQDMENEPATSYGFSIQLDNEFDY
                     YTSLIFASSFNSNESKHPKLDITYTGQNEVNNYYTNSFSISPNPARSFINVNFSKSTT
                     TPVVMNVFDAIGNVVKTVNVPNNSNEINLEISDLADGIYLIGNNDGYSKKFVITK"
BASE COUNT          612 a          285 c          348 g          539 t
ORIGIN      
        1 atttagcatc gggtgttccg ggaagtgtag ccggcatgtg ggaagcacat caaaaatatg
       61 gcagcatgga ttggaaaaaa ttgttgcaac ctgctattga attagcccaa aaaggttttc
      121 ctattacggc gcatcaggca gatgatttta atcaactaca atcagagtta aaacaattaa
      181 ataaaggaaa aaattatttt attaaatcaa aatggaatgc gaatgacaca ttaattcaaa
      241 ctgatttagc tactacctta attgccattc gcgataaggg cagagatgga ttttataccg
      301 gacctgtagc tgaaaaaatt gctgcagaaa tgcaacaaca taatggtata atttctactg
      361 atgatttaaa aaattatcag gcagtttggc gtgaccccat aaaaggctct tataaaaatt
      421 acactattat aagtatgcca cctccttcaa gtggtggaat tgccttaatg caattattaa
      481 atatgatgga aaattatcca ttaaaaaaat ggggattcca ttctgtaaat agtgtatcca
      541 ttatgacaga agcggaaaaa cgcgtgtatg ccgaccgcgc tacctggttg ggagatcctg
      601 attttactaa agtgccgaaa aatgaattaa taagtaaaac ctatgcggct tcgcgtatgc
      661 aaaatgtgga cacaaataca ataactcctg cagcacaaat taaagcagga acttttaccg
      721 gttatgaaag tgaagaaaca acccatttta gtatcgtaga taaatatggt aatgctgtat
      781 caattacaac tacgttaaat gacagctatg gctctcgcat aatagtaaat ggatgcggat
      841 ttattttaaa taatgaaatg gacgatttta gtgcaaaacc aggtgagcca aatttatatg
      901 ggttaattgg tggtgaagct aatgcaattg ctcccggaaa acgtatgttg agcagtatga
      961 caccgactat tgttgaaaaa gaaggtaaat tatttatggt agttggaaca cccggtggct
     1021 caacaattat taccagcgtt tttcaaaata ttatcaatgt aattgaattc gacatgacta
     1081 tgcaggaagc agttgatgcg cctcgttttc ataatcagtg gttacctgat gaaattaaag
     1141 ttgaaaatga ttttaacgaa acaaccgttg ctgcattaaa agccaaaggt tataaaataa
     1201 taggtcgtga agccattggc cgtgttgatg caattttggt ttatccgaac ggcaagctgg
     1261 aaggagccgc tgataaacgt ggggatgatg ttgctgttgg ttggtaaatt actttgtaat
     1321 tacaaacttt ttgctgtaac cgtcattatt acctattaag taaattccgt cagccaaatc
     1381 tgatatttcc aagttaattt cattagaatt atttggaaca ttaacagttt tcacaacatt
     1441 accaatcgcg tcaaaaacat tcattactac tggagtagtt gtgctcttcg aaaaatttac
     1501 attaataaat gaccgcgctg gatttggaga tatggaaaaa ctatttgtat aataattatt
     1561 aacttcattt tgtcccgtgt aagtgatgtc aagctttggg tgtttagact cattcgaatt
     1621 aaatgaagat gcgaaaatta agctagtgta ataatcaaat tcgttatcta attgaatact
     1681 aaaaccatac gaagtggctg gttcattttc catatcttga actagttcaa taacatctaa
     1741 ttcataatcc tgaattggat cagtacttgt cgggatagca accg
//
LOCUS       DAORKW010000685         1781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4434_length_1781_cov_4.101970, whole genome shotgun sequence.
ACCESSION   DAORKW010000685 DAORKW010000000
VERSION     DAORKW010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4434_length_1781_cov_4.101970"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /locus_tag="PLB46_16950"
     CDS             <1..1755
                     /locus_tag="PLB46_16950"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HRF78270.1"
                     /translation="KGTNTQKVGDFWFSGMDTVAIEKAGIDPIKPWLEKINAISTTDD
                     MIKTVAQMHRTYTFALFTNWVGQDDKNSADVIMNMWQGGLGLPEREYYLAQDAQSKQI
                     RADYINHIKNVFVLMGIDEAKATANANTILTVETAMAKASMDQVTMRDPNAIYHKMSV
                     EEFAKMTPNINWNLYYAELKTPEFKTGINVAQPEFYKALNGMVKSVKMDDWKTYLTYH
                     MVDGASPYLSSNFVNENFDFHSKKLNGIEQIKPRWKRVVETADFCLGEALGEEYIKTA
                     FSPESKARMLEMVDNIKVAFGERIKTLDWMTDSTKTEALNKLSTFTVKIGYPDKWRDY
                     SKLEIDRGAYVLNVIRAAEFEANRNYAKIGQPVDKNEWGMTPQTVNAYYNPSNNEIVF
                     PAAILQPPFFDPKADDAVNYGGIGAVIGHEITHGFDDQGRMYDAKGNLQMWWKDEDNT
                     KFVERANRVVDQFNSYCPLDSVCINGRLTLGENIADFGGITIAYQAYTMTEEFKAAQE
                     IAGFTPQQRFFIGFGRIWAGQYRESAMRTQLLTNVHSPGKWRVNGTVVNIPEWYQAFG
                     IKEGDKMYLPETDRAKIW"
BASE COUNT          614 a          298 c          358 g          511 t
ORIGIN      
        1 aaaggcacta acacccaaaa agtaggcgat ttctggttta gtggtatgga tactgtagct
       61 attgaaaaag cgggtattga tcctatcaaa ccttggttag aaaaaattaa tgctatcagc
      121 actactgatg acatgattaa aactgttgct caaatgcacc gcacttacac atttgcttta
      181 ttcaccaatt gggttggtca ggatgacaaa aattctgctg atgtaattat gaatatgtgg
      241 caaggtggtt taggtttacc agaacgcgaa tattacctcg cacaagatgc acaaagcaaa
      301 caaatcagag ccgattatat caatcacatt aaaaatgtat ttgtgttaat gggtattgat
      361 gaagcaaaag ctactgcaaa tgcaaacacc attttaactg ttgaaacagc aatggctaaa
      421 gcaagtatgg accaggttac catgcgcgac cctaatgcca tttatcacaa aatgtctgtt
      481 gaggaatttg caaaaatgac tccgaatatt aattggaatt tatattacgc agaattaaaa
      541 acacctgaat ttaaaacagg tatcaatgtt gctcaacctg aattttacaa agctttaaac
      601 ggcatggtaa aatctgtaaa aatggatgac tggaaaacct atttaaccta ccacatggtt
      661 gacggtgctt ctccatattt aagcagcaat tttgttaatg aaaattttga tttccatagc
      721 aaaaaattaa acggtatcga acaaattaaa ccaagatgga aacgtgtagt tgaaactgca
      781 gacttttgtt taggtgaagc acttggtgaa gaatatatta aaacggcatt tagtccggaa
      841 agtaaagcac gtatgctcga aatggttgac aatattaaag ttgcctttgg cgaacgtatt
      901 aaaactttag attggatgac tgacagtact aaaactgaag cattaaataa attaagcaca
      961 tttacagtta aaattggtta tcctgataaa tggagagact atagcaaatt agaaattgac
     1021 cgtggtgctt atgtattaaa tgtaattcgt gctgctgaat ttgaagctaa tcgcaactat
     1081 gcaaaaattg gtcagccggt tgataaaaat gaatggggta tgacacctca aacagtaaat
     1141 gcctattaca atccttctaa taatgaaatt gtattccctg cagctatttt acaaccaccg
     1201 ttttttgacc ctaaagctga tgatgctgta aactatggcg gaattggtgc ggtaatcggt
     1261 cacgaaatca cccacggctt cgacgaccaa ggtcgtatgt atgatgcaaa aggtaaccta
     1321 caaatgtggt ggaaagatga ggacaataca aaatttgttg aacgcgcaaa tcgtgttgtt
     1381 gaccaattta acagctattg cccattagac agcgtttgta ttaacggtag attaacctta
     1441 ggcgaaaaca ttgccgattt tggtggtatc actattgcat atcaggcata taccatgact
     1501 gaagaattta aagcagcgca ggaaattgca ggctttactc cgcaacaacg tttctttatt
     1561 ggttttggtc gtatttgggc cggtcaatat cgcgaaagtg ctatgcgtac acagttatta
     1621 accaacgtgc atagtcctgg caaatggcgt gtaaacggaa cagtggtaaa tattcctgaa
     1681 tggtaccagg catttggtat taaagaaggt gataaaatgt atttacctga aacagatcgt
     1741 gctaaaatct ggtaataatc gctattaaaa tatttaaaaa g
//
LOCUS       DAORKW010000686         1780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4442_length_1780_cov_2.433043, whole genome shotgun sequence.
ACCESSION   DAORKW010000686 DAORKW010000000
VERSION     DAORKW010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4442_length_1780_cov_2.433043"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLB46_16955"
     CDS             <1..783
                     /locus_tag="PLB46_16955"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HRF78271.1"
                     /translation="RAAPPVFGLGLLEAINSFDIIATADENDNNQDGISGKVNYVYDV
                     VSGTNQIGRFGWKANQPHLDQQTAGAFSGDMGITTPLFPQESCSGQLQDDGLGDDPEV
                     DAVFLDVTSFYTKTLAVPAPRNLDNPQVQKGKQLFFEIGCENCHRQKWTTGVLADIPE
                     VSNQTIYPYTDMLLHDMGPGLADNRPDYLADGYEWKTRPLWGIGLTALANGHTRFLHD
                     ARARNLEEAILWHGGEAQKSTDAFKNLSVTERTNLIKFLEAL"
     gene            969..>1780
                     /locus_tag="PLB46_16960"
     CDS             969..>1780
                     /locus_tag="PLB46_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRF78272.1"
                     /translation="MISNRSSAMEQVLNREAIEVKVPSTKISDYYGSNVFSEEIMRKH
                     LSEEAYFSVKSSIQQGQKIDRKIADQVAASMKAWAMSKGATHYTHWFQPLTGTTAEKH
                     DSFFTPSGPAAGIEVFSGGALVQQEPDASSFPSGGIRSTFEARGYTAWDPSSPAFIME
                     LGSGSTLCIPTIFVSYTGEALDYKAPLLKSLHLLDEAAVPVCQYFDKNVTRVTATLGW
                     EQEYFVVDEALFLNRPDLSICGRTLIGHYPAKGQQLEDHYFGTIPERVFNYMV"
BASE COUNT          541 a          357 c          374 g          508 t
ORIGIN      
        1 cgggcagctc caccagtttt tggacttggg ttgttagaag ccataaacag ttttgatatc
       61 atagctaccg ctgatgaaaa tgataataat caggatggca tttccggaaa agttaattac
      121 gtatatgatg tagttagcgg aaccaatcag ataggtcgtt ttggatggaa agccaatcaa
      181 ccacacctcg atcagcaaac ggcaggtgca tttagtggcg acatgggtat aaccacgcct
      241 ttgtttcctc aggaatcatg cagtggccag cttcaggatg atggtttagg cgatgatccg
      301 gaagttgatg cggtgttttt agatgtaact tctttttata ccaaaacttt ggcagtccct
      361 gcccccagaa atctagacaa ccctcaggtg caaaaaggca aacaactatt tttcgaaatc
      421 ggttgcgaaa attgccatcg ccaaaaatgg accaccggtg ttttagcgga tattcctgaa
      481 gtgagtaacc aaaccattta cccgtacacc gatatgttac tccacgatat gggtcctggg
      541 ttggccgata accgacctga ttatttggcg gatgggtatg aatggaaaac acgaccgctt
      601 tggggcatag gactaacggc acttgctaat ggccataccc gtttcttgca cgatgcaaga
      661 gcgcgcaact tggaagaagc tattctatgg catggcggtg aagctcaaaa atctaccgat
      721 gcgtttaaaa acctaagtgt aacagaaagg actaacctca tcaaattttt agaagcactt
      781 tagttttttc aatttcatat tatatttatt tatatatata ctatttcaca atcaactcat
      841 cgtttttacc atttttccag aaaataacac agaaatttta agctctttgg cgattttcca
      901 cataaaaagt ggacaattga tgtacatttt cgaaaattcc cgataatttt gtaaaaaaat
      961 taaccggaat gatatcaaac aggtcctctg ctatggaaca ggtgcttaat cgcgaggcga
     1021 ttgaggtaaa ggtcccttcc accaaaattt ctgattatta tggctcgaat gtgtttagcg
     1081 aagaaattat gcgcaaacac ctgagtgaag aagcgtattt cagtgtaaaa tcttccattc
     1141 agcaaggcca aaaaattgac cgcaaaattg ccgaccaggt agctgcgagt atgaaagcat
     1201 gggcaatgag caaaggtgcc acacattata cacattggtt tcagccatta acaggaacta
     1261 ctgctgaaaa acatgatagt ttcttcacac caagcggccc tgcagcagga attgaagtat
     1321 ttagcggtgg agcactcgtt caacaagaac ccgatgcatc gagttttcca agtggtggaa
     1381 ttcgcagtac tttcgaagcc cgaggatata cagcatggga tccatcatca cctgctttta
     1441 ttatggaatt aggaagcgga agtactttat gtatccctac catttttgtt tcctacaccg
     1501 gtgaggcatt agattataaa gcacctttat taaaatcatt acacttactc gatgaagcag
     1561 ctgttcctgt ttgtcagtac ttcgataaaa atgttacccg cgttaccgca actttaggtt
     1621 gggaacaaga atattttgta gttgatgagg cattattttt aaatcgtcct gacttaagta
     1681 tttgtggcag aacattaatt ggtcattacc ctgcaaaagg tcagcaatta gaagaccatt
     1741 atttcgggac catacctgaa cgtgtattta attacatggt
//
LOCUS       DAORKW010000687         1760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4523_length_1760_cov_4.850440, whole genome shotgun sequence.
ACCESSION   DAORKW010000687 DAORKW010000000
VERSION     DAORKW010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4523_length_1760_cov_4.850440"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PLB46_16965"
     CDS             complement(<1..1154)
                     /locus_tag="PLB46_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78273.1"
                     /translation="MKIIYFILLTASSLIIHAQFPVLNWDKTIGGQYYDVVSSFLETS
                     DGNFLIGGTSNSNASAYKSENALGMNEFDETTFDFWVIKVNSSGEILWENTIGGSKDD
                     YLYSIVACPDGGFILAGASYSNNSFDKTEDCKGNYDYWIVKIDDSGDVLWDNTIGGAA
                     IDLLHDIKLTNDGNYILAGYSGSGISEDKTENYIPGGTFHWDYWVIKISPTGDIIWQN
                     TIGGNFDDVLLKVIPTDDDGFLLGGYSKSLPGYDKVASYYGLNDMWLVKLNSDGSIAW
                     QKSYGGMSEDYLYSMESCSDGGYILGAQSKSAISGVKTEGVIGGGVAYDYWVVKIDSI
                     GNIEWQNTIGSNIQDHLYFVTESEEGNAYYVCGDSDFGISGDKTDAGNGGT"
     gene            complement(1312..>1760)
                     /locus_tag="PLB46_16970"
     CDS             complement(1312..>1760)
                     /locus_tag="PLB46_16970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78274.1"
                     /translation="AGCSYQWYKDASPIPGATATNYLATTAGIYKCRVTKIVTGCYKT
                     SSGITVTVPCKENASAALNIFSIFPNPNSGTFTISANLIAPIGTNTTAKLEIYNAIGQ
                     LIYYKELPATGDQFYQQVSLDVFPSGVYLVNLLLGNKQSTHQLIIE"
BASE COUNT          571 a          354 c          241 g          594 t
ORIGIN      
        1 gttcctccat tccctgcatc agttttatca cctgaaatgc caaaatccga atccccacaa
       61 acataatatg cattaccttc ttctgattct gttacaaaat ataaatgatc ttgaatgttt
      121 gaaccgattg tgttctgcca ttcaatatta ccaattgaat caattttcac tacccagtaa
      181 tcataagcaa ctccaccccc aataacacct tcagttttaa ctccggaaat agcagatttt
      241 gattgagcac ctaaaatgta acccccatct gaacaggatt ccattgaata taaataatct
      301 tctgacattc caccatatga tttttgccaa gcgattgaac catcgctgtt taactttact
      361 aaccacatat catttaatcc gtaatatgaa gcaactttat cataaccggg taatgattta
      421 gaataaccgc caagtaaaaa accatcatca tcagttggaa ttacttttaa caaaacatca
      481 tcaaagttcc ctcctattgt attttgccat ataatatcgc ctgttgggct tattttaata
      541 acccaataat cccagtgaaa tgtgccacct ggtatataat tttccgtttt atcttcagaa
      601 attccggaac ccgaataacc tgcaagaata taatttccat catttgtgag ctttatatca
      661 tgcaataaat ctatcgctgc tccaccaata gtattatccc ataaaacatc acctgaatca
      721 tcaattttta caatccaata atcataattg cctttacaat cttcagtttt atcaaaagaa
      781 ttattagaat atgatgctcc ggcaagtata aaaccaccat ctggacaggc aactatgctg
      841 tataaataat catcttttga acctccaatt gtattttccc acaatatttc tccactacta
      901 ttaaccttaa ttacccagaa atcaaatgtt gtttcatcaa attcattcat gcccaatgca
      961 ttttcacttt tatatgcaga ggcatttgaa ttagatgttc ctcctatcag gaaatttcca
     1021 tccgaagttt ctaaaaagga gctcaccaca tcataatatt gtcccccaat tgttttatcc
     1081 cagtttaaga cagggaactg tgcatgtata attagggaac tggcggtcaa tagaatgaaa
     1141 taaataattt tcataattaa tttattttaa taggaatata aatctttaaa ataacttcaa
     1201 tttgcttttg caaagtaata aaagtatctc tctattccct tacatttaat aaaatttatt
     1261 tattgaccat ttgcttgaaa aacactttta taggaacatt cttgtatgtc tttattcaat
     1321 tattaactga tgtgttgatt gcttatttcc gagcaataaa tttaccaggt aaacgcctga
     1381 aggaaataca tctaaagata cctgttggta aaattggtct ccagtagcag gcaattcctt
     1441 ataatagatc agttgaccga tggcgttata aatttctaat tttgctgttg tattggttcc
     1501 aatgggagca attaagtttg ctgaaattgt aaatgtaccg ctgttagggt taggaaaaat
     1561 ggaaaaaata tttaatgctg cggaagcatt ttctttgcat ggcactgtca ccgtaattcc
     1621 tgatgaagtt ttataacagc ctgtgactat ttttgttaca cggcatttat aaatacctgc
     1681 tgtagttgcc aaataattag tcgctgttgc acccggaatt ggcgatgcat ctttatacca
     1741 ttgatagctg catcccgcaa
//
LOCUS       DAORKW010000688         1758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4540_length_1758_cov_4.165003, whole genome shotgun sequence.
ACCESSION   DAORKW010000688 DAORKW010000000
VERSION     DAORKW010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4540_length_1758_cov_4.165003"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..>1758
                     /locus_tag="PLB46_16975"
     CDS             7..>1758
                     /locus_tag="PLB46_16975"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF78275.1"
                     /translation="MESINEMTTVSARQFSIEESQLFAGARNDVSRMAQNFAGVRGAN
                     DAVNDIIIRGNSPVGLLFRLEGMDIPNPNHFGDLGSSGGPVSMLNNNVLANSDFLTGA
                     FPAEYGNAISGVFDLRMRNGNYNKHEFVGQVGLNGFEFGAEGPISKSSKSSYLLNYRY
                     STLGFMSALGINFGTGTAIPYYQDITTRFNFPTEKAGTFSVFAIGGVSQIDLISSNEE
                     DTVDNLYNNDLDIYDRSKTGIAGISHQYLIDNTSYTKFTLAASYVMNSDLIDSISPET
                     LDPVPFYSQNFTQTKIAGNFFYKKKFNTKHNLQIGVRFDEFLVDIVDSIYRSTIDRYI
                     TLTEYDGNTFLLQPYAQYQYRVSDKMSLNAGLHYQYLGLNGSSSLEPRAGIKYQINEK
                     NALSFGYGLHSMMAPIATYFKTVEVSVGNYITPNDSIDFVKAHHVVLGYDLQLSPTIR
                     LKTEVYYQHLYDVLIDKDSSSYSVLNTGSLTFGSPDYLKNSGTGRNYGAELTLEKFMD
                     KGMYFLVTGSLYRSEYTGSDDIQRESAFSGNYVTNFVGGKEFNLFPNRVGQKTKKSIV
                     LDTKFTLAGGQRYTPIDL"
BASE COUNT          558 a          297 c          343 g          560 t
ORIGIN      
        1 gagaaaatgg aatcaataaa cgaaatgacc accgttagtg ctcgtcagtt ttctattgaa
       61 gaaagtcagc tatttgccgg tgcaagaaac gatgtgagtc gcatggcaca aaactttgcc
      121 ggtgtgcgtg gggcaaatga tgccgtaaat gatattatca ttcgcggaaa ttcccctgtt
      181 ggattattat ttcgtttaga aggaatggat atccctaatc ctaatcactt tggtgattta
      241 ggttcaagtg gtggtccggt ttcaatgctc aataataacg tattagcaaa cagcgatttt
      301 ttaaccggag catttcctgc agaatatggc aatgcaatta gtggggtgtt tgatttacgc
      361 atgcgcaatg gtaattataa caaacatgaa tttgttggac aggttggatt aaatggtttt
      421 gaatttggtg cagaaggtcc tattagtaaa tcatcaaaat catcttattt actaaattac
      481 cgttattcta ctttaggttt tatgtcggct ttgggaatta actttggaac aggaactgcc
      541 atcccatact atcaggacat tacaacacgg tttaatttcc ctacagaaaa agcaggaacg
      601 ttttccgtat ttgctattgg tggcgttagt cagatagatt taatttcgag taacgaagag
      661 gatacggtag acaatttata caataacgac ctcgatattt acgatcgttc taaaacagga
      721 attgccggta tcagccatca atatttgatt gacaatactt catataccaa attcactttg
      781 gctgcttcat atgtaatgaa cagtgattta attgattcaa tttcacctga aacgcttgac
      841 ccggttcctt tctattcaca aaattttacc caaacaaaaa ttgccgggaa tttcttctac
      901 aaaaagaaat ttaataccaa acataattta caaatcggcg taaggtttga tgaatttttg
      961 gtggatattg ttgacagcat ttatcgttca actattgacc gttacattac tttaaccgaa
     1021 tatgatggta ataccttttt attacaacca tatgcacaat atcaataccg tgtttcagat
     1081 aagatgagtt taaatgcggg tttgcattat cagtacctcg gattaaatgg aagtagctcg
     1141 ctagaaccac gtgcaggtat aaaataccag ataaatgaaa aaaatgcact gagttttggt
     1201 tatggtttgc atagtatgat ggcgcctatt gctacttatt ttaaaacagt agaagtcagc
     1261 gttggcaatt atattacacc aaacgattct atagattttg ttaaagcaca ccacgtggtg
     1321 ttaggatatg atttacaact gtcgccaaca attcgattga aaacggaagt ttattatcag
     1381 catttatatg atgtgttgat agataaagat agttcttcct attctgtgtt gaataccggc
     1441 agccttacat ttgggtcacc ggattatttg aaaaatagcg ggacaggtcg caattatggc
     1501 gcagaactta ccttggagaa atttatggat aaaggcatgt atttcctcgt aaccggttca
     1561 ttatacagat cagagtatac aggaagtgat gatattcaac gcgaatctgc attttcaggt
     1621 aattatgtaa ccaattttgt tggtggaaaa gaattcaacc tcttccctaa ccgtgttggt
     1681 caaaaaacaa aaaaatctat tgtattagat accaaattca ctttagcggg tggacaacgt
     1741 tataccccaa tagattta
//
LOCUS       DAORKW010000689         1745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4611_length_1745_cov_2.957988, whole genome shotgun sequence.
ACCESSION   DAORKW010000689 DAORKW010000000
VERSION     DAORKW010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4611_length_1745_cov_2.957988"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..>1745
                     /locus_tag="PLB46_16980"
     CDS             106..>1745
                     /locus_tag="PLB46_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78276.1"
                     /translation="MSLISMGITLGVVLLSAILIPLLFEDQIKGLFIRELNKNLATEI
                     TINQDDIHLSLLKNFPNAAVVFNNVGIRESVPLKGGVKGKSDKNFLEAEEISLVFNVW
                     DIFKGNYTIKHIQVKNGFCHLITDKKGNINYKFWKDSSGESASDFSINLQQVDCENVD
                     FQYLDYKYEQNVEMLIHDCGFTGNFSSDNYLMELDGSVLSKRIKIGGTAYLVNKEAQI
                     NTGINVNVPDDNYAFEDGKITIDKNTFLIDGNINLKDEDYYDLAINGDKISMEGLMLL
                     LPGSIANNLSKYKSRGKIDFATTIKGNYTSTKTPQITITFDVNGASIEHEKFGGKLSN
                     MQFSGKYSNGEKHNAASSYISILNFKAEQNNLPVTLSLNYKNFSNPNIDLLLDGSFPA
                     SLIIPLAMSNASDVEGTIGLNDINIKGNIKTLSSQLNTNKPTGTITFNDVSFVVNDNK
                     ISIPTGKATVINNEVNLNGLNIDFAGSDLKVDLTINNWIENVFPAEYKPALNINGDIH
                     SDKINLNTLIAIFDTKETTTKSAPAQRDVGAANLEASSS"
BASE COUNT          569 a          337 c          345 g          494 t
ORIGIN      
        1 tttttcatct gctgcgttta gatagtcgtt gtaactttga acgtggaaga aattgtaccc
       61 aacctcaacc cccaacctaa gccaaaatcg cggaaaatca tgcgcgtgag cctcatttcg
      121 atgggaatca cccttggcgt ggtgttgctg tctgcaatcc tgattccgct gctgtttgag
      181 gaccagatta aagggctgtt tatccgggaa ctcaataaaa acctcgctac cgaaataacc
      241 attaaccagg atgatattca tctgagcctg ctcaaaaact tccccaatgc cgctgtggta
      301 ttcaataacg tgggtattcg cgaaagcgtg cctttaaaag ggggtgtaaa gggcaagtcc
      361 gacaaaaatt tccttgaggc ggaggaaatc agtctcgttt ttaacgtctg ggacatcttt
      421 aaaggcaatt acaccatcaa acatattcag gtgaaaaacg ggttttgtca cctcattaca
      481 gacaaaaaag gcaatatcaa ctataaattc tggaaggaca gctccggtga aagtgcgtcg
      541 gatttttcga taaacctaca gcaggtggat tgcgaaaacg tggattttca gtatctggat
      601 tataaatatg agcaaaatgt ggaaatgctg attcacgact gcgggtttac cggcaatttt
      661 tcttcggata attacctcat ggaactggat ggcagtgttt tatcgaaacg gataaaaatt
      721 ggtggaacgg cttatctcgt aaataaagaa gcacaaatta ataccggcat caacgtaaat
      781 gttccggacg ataattatgc ttttgaagac ggaaaaatta ccatcgataa aaatactttt
      841 ctgattgatg gtaatattaa tttgaaagac gaggattatt atgacctggc catcaacggc
      901 gataaaatta gtatggaagg cctcatgtta ttgttacccg gctccatcgc caataacctg
      961 agcaaatata aaagtcgcgg gaaaatagat ttcgctacca ccattaaagg caattacact
     1021 tccaccaaaa cacctcaaat taccatcaca tttgatgtaa acggcgcgag tattgaacat
     1081 gaaaaatttg gaggcaaact gagtaatatg cagttcagcg gaaaatattc caatggtgaa
     1141 aaacacaatg ctgcttcatc ttatatttcg atattaaatt ttaaagcaga acaaaataat
     1201 ttaccggtaa ccttatcgct taactataaa aatttcagta atccgaatat cgatttattg
     1261 ttagatggga gttttcctgc ttctctgatt attccgcttg caatgagcaa tgcatctgat
     1321 gtggaaggga caattggttt aaatgatatc aatattaaag gcaatatcaa aacgctttcc
     1381 agtcagctca atacgaataa accgacaggt accatcactt ttaatgatgt cagttttgtg
     1441 gtgaacgata ataaaatttc catcccaacc ggtaaggcga ctgttattaa taatgaagtg
     1501 aatttaaacg gattaaacat cgattttgca ggttccgatt taaaagttga tcttaccatt
     1561 aacaactgga ttgaaaatgt ttttcctgcg gaatataaac cggcattaaa tataaacggt
     1621 gatatccact ccgataaaat taatttgaat acactcattg ccattttcga tactaaggaa
     1681 accacgacca aatcagcgcc ggctcaacgt gatgttggtg ctgcgaatct tgaagcttca
     1741 tcttc
//
LOCUS       DAORKW010000690         1737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4646_length_1737_cov_3.534483, whole genome shotgun sequence.
ACCESSION   DAORKW010000690 DAORKW010000000
VERSION     DAORKW010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4646_length_1737_cov_3.534483"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PLB46_16985"
     CDS             <1..1093
                     /locus_tag="PLB46_16985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRF78277.1"
                     /translation="NMHLLTGEYTFRWLKTINPHLDFAFSNISSVEQNTNYGKKRIVP
                     DAWLAESSGSGYLKFHYTKSVFEIGVGGGMRYIKTLLTPGVNTPDKDIDPFTIYRPFV
                     TGLAGWSFFPSQKWNLKTNISTGVRSPNLAELSSNGLHEGVFTYDIGDPTLENEKNIN
                     SELSIDYNGDIFQAYAGVFYNHFIDYIYIEPTGEEWFGFPIYRYKQQTANLSGFEVEL
                     SCTPSAIKNTTARIAYSAMQGVKADGSNLPFIPANKLTPELKYTVPQFKSAENIYAFV
                     NIDFVFGQYNTAAYESSTPQYVLLNAGAGFVLRTTHFPIDISLTGNNLLNTVYVDHLS
                     RFKDLGYKNIGLNLVLNIKIILSKQIKTN"
     gene            1095..1526
                     /locus_tag="PLB46_16990"
     CDS             1095..1526
                     /locus_tag="PLB46_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78278.1"
                     /translation="MKKQFQVLTFLLISGLAITFTSCNKEDEMEPPTISFKTGAGYTS
                     ANANVTAGNSILIGIEAAKSDSEEESEDVLTHFNITKSVNGGTGTSVYDIDVPAANED
                     DYDYDFNTTTGATVGDTEKYTFTITNRDGLTGQISLTITVQ"
BASE COUNT          589 a          286 c          313 g          549 t
ORIGIN      
        1 aaatatgcat ttgttaaccg gagagtacac atttcgttgg ttaaaaacaa ttaacccgca
       61 tttagatttc gccttttcca atatttcaag tgttgagcaa aatacgaatt acggtaaaaa
      121 aagaattgtg cccgacgctt ggttagcaga aagtagtggt tccggatatt tgaaatttca
      181 ttatacaaaa tctgtgtttg aaataggcgt tggtggtggt atgcgatata ttaaaacatt
      241 gcttacaccg ggtgttaata caccggataa ggatattgac ccattcacta tttatcgtcc
      301 gtttgttaca ggacttgcag ggtggagttt ttttccatca caaaaatgga atttaaaaac
      361 gaatatcagt accggtgtac gcagtccgaa tcttgctgaa ctttcatcta acggtttaca
      421 tgaaggcgta tttacttatg atattggtga tccaacatta gaaaatgaaa aaaatatcaa
      481 ttccgaatta agtattgatt ataatggcga tatatttcaa gcttatgcgg gggtatttta
      541 caatcatttt atagattaca tttatattga accaacaggt gaagaatggt ttggatttcc
      601 gatttatcgt tataaacaac aaactgcaaa tttatccggg tttgaagttg agttaagttg
      661 tacaccttct gcaattaaaa atacaacagc tcgaattgca tattctgcta tgcagggagt
      721 taaagcagat ggttccaatt tgccttttat tccggcaaat aaattaacac cggaattaaa
      781 atatactgtc ccgcaattta aatctgcaga aaatatttat gcttttgtaa acatcgattt
      841 tgtttttgga cagtataata ccgcggcata tgaatcatct accccccaat atgtattact
      901 aaatgcagga gctggttttg ttttgcgcac tacacatttt ccgattgata tcagcttaac
      961 aggaaacaac ttactcaaca cggtatatgt tgatcattta tcccgcttta aagatttagg
     1021 ttataaaaat attggcttaa atctcgtatt aaatattaaa attattttat caaaacaaat
     1081 taaaactaac taaaatgaaa aaacaatttc aggtattaac attcctgctg attagtggat
     1141 tagcaatcac atttacatct tgcaataaag aggatgaaat ggaaccacca acaatcagtt
     1201 ttaaaacagg tgcgggatat acttccgcaa atgctaatgt aactgcaggc aactcaatat
     1261 taattggtat tgaagcagca aaatcagatt cagaagaaga aagtgaagat gtattaacgc
     1321 actttaatat taccaaatca gtaaatggtg gaacaggaac ttctgtttac gatattgatg
     1381 taccagctgc aaatgaagat gattatgatt acgacttcaa cacaaccaca ggagcaacag
     1441 ttggtgatac agaaaaatac acttttacca taaccaatcg tgatgggtta accggacaaa
     1501 ttagtttaac aattacagta cagtaagttt tacaaaagcg aaggccggat gcaattaagc
     1561 gtccggcctt tttttatttt ttagagattt aattttcgtt gtcggatgtt agccagaaat
     1621 tatttggacc ttcacctacc aataatgtta gtttttgctg tgcaattaag tctaacacgc
     1681 tgaggaaacg gaacactgcc tgatatctgt ctttgcagga tgtgaatact tcagcaa
//
LOCUS       DAORKW010000691         1735 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4655_length_1735_cov_3.344643, whole genome shotgun sequence.
ACCESSION   DAORKW010000691 DAORKW010000000
VERSION     DAORKW010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4655_length_1735_cov_3.344643"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PLB46_16995"
     CDS             <1..897
                     /locus_tag="PLB46_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRF78279.1"
                     /translation="VVGQDEAIVAVADAIRRSRAGLQDPKKPIGSFIFLGTTGVGKTE
                     LAKAISGYLFNDDQAMTRIDMSEYQERHAVSRLIGAPPGYVGYDEGGQLTEAVRRKPY
                     SVILLDEIEKAHPDVFNILLQVLDDGRLTDNKGRTVDFKNTIIIMTSNIGSHIIQERF
                     EGVTENNLLATTETTKIEVMEMLRKTIRPEFLNRIDEIIMFKPLLRNEIRGIVKIQLA
                     GLANMLEERSIKITFTETLIDWLANEGFDPQFGARPLKRVIQKEIVNALSKKILASEV
                     VTDDVIRVDVKNDVVVFENTRG"
     gene            1016..1372
                     /locus_tag="PLB46_17000"
     CDS             1016..1372
                     /locus_tag="PLB46_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78280.1"
                     /translation="MKTGQYKFLLSLAVIIGLFAGCTKLDLPITVPNCVEQDIKRFER
                     QDGGFDNAVAVFQWQVDGKIYYYYVYQCCDQFNYLYDEDCNLVCAPVGGFTGDGDGNC
                     PEFVGDITSVLVWEKE"
     gene            1480..>1735
                     /locus_tag="PLB46_17005"
     CDS             1480..>1735
                     /locus_tag="PLB46_17005"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78281.1"
                     /translation="MKKTIGLGLLFTLFSSAVFSQTADPFMWLEDVDSERSMEFVNQH
                     NTGSVDVLNKLPTYQPIYDKCLEIYNSTDRIVYPTTYGDFI"
BASE COUNT          533 a          263 c          398 g          541 t
ORIGIN      
        1 gtagtggggc aggacgaagc cattgttgcg gttgcagatg ccattcgcag aagcagagcg
       61 ggtttgcagg accccaaaaa accaattggc tcatttattt ttttgggaac aaccggtgtg
      121 ggaaaaacag aattggcaaa agctatttcc gggtatttat ttaacgacga tcaggcaatg
      181 acaagaattg atatgagtga atatcaggaa cgtcacgcag tgtcgcgatt aattggtgcg
      241 cctccgggat atgttggtta tgatgaaggc ggtcaattga cagaagcggt gagaagaaaa
      301 ccgtattcag ttattttatt ggatgaaatt gaaaaagcac atcccgatgt atttaatatt
      361 ttattacagg tattggatga tggaagatta acggataata aaggcagaac cgttgatttt
      421 aaaaatacca ttattatcat gaccagtaat attggttcac atattattca ggagcgattt
      481 gaaggggtaa cggaaaataa tttattagca acaactgaaa ctactaaaat tgaggtaatg
      541 gaaatgttgc gcaaaacaat ccgtcctgaa ttcctaaatc gtattgatga aatcattatg
      601 ttcaaaccac tgttgcgcaa tgaaattcgt ggaattgtaa aaatacagtt agcaggtttg
      661 gcaaatatgc tggaagagcg ttcaatcaaa ataacattta cggaaacact tatcgattgg
      721 ttagccaacg aaggatttga ccctcagttt ggtgcgagac ctttaaaaag ggttattcaa
      781 aaggaaattg taaatgcttt gtcgaaaaaa atattggcga gtgaggtggt tactgatgat
      841 gttattcggg ttgatgtgaa aaatgatgtg gtggtgtttg aaaatacacg tggataatgt
      901 aaggtggttc acgcggagaa ggggagaggg cggaagtgtt agatggaggt ttttttgggg
      961 aagtgtgcaa taaattttgt atttttcgtt cactttgaaa tttaactcca aaagtatgaa
     1021 aaccggccag tataaattcc tgctttccct tgcggttata atcggccttt ttgccggctg
     1081 taccaagtta gatttgccaa ttacggttcc aaattgtgtt gagcaagaca ttaaaagatt
     1141 tgaaaggcaa gatggtggtt tcgataatgc agttgctgtt tttcaatggc aggtagatgg
     1201 taaaatttat tattattacg tgtatcaatg ttgcgatcag tttaattatt tatacgacga
     1261 ggattgtaac ctggtatgcg cacctgttgg tggttttacc ggtgacggtg atgggaattg
     1321 ccctgaattt gttggtgaca tcaccagtgt tctcgtgtgg gaaaaagaat aagcatccgg
     1381 ccctccacaa aatgttaaat acttccttta tgctgcaacc gaaaccggta tagatttctt
     1441 ttgttatttt tatgcaaagt ttaacttaca attttgcata tgaaaaaaac aatcggctta
     1501 ggtttattat ttacactttt ttcttcagct gtttttagtc agacagcaga cccctttatg
     1561 tggctcgaag atgttgacag cgaacgttca atggagtttg ttaatcaaca caataccggt
     1621 tcagttgatg tattaaataa attaccaact tatcagccaa tttatgataa atgtctggaa
     1681 atttataact ctaccgaccg tattgtttac ccaaccacgt atggcgattt tattt
//
LOCUS       DAORKW010000692         1732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4669_length_1732_cov_5.589744, whole genome shotgun sequence.
ACCESSION   DAORKW010000692 DAORKW010000000
VERSION     DAORKW010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4669_length_1732_cov_5.589744"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1323
                     /locus_tag="PLB46_17010"
     CDS             58..1323
                     /locus_tag="PLB46_17010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRF78282.1"
                     /translation="MHCIIQGVKIIDPTSPYHLSVKSIRIHNGVIAEIADVINNSDAQ
                     VYDYTGNSVSPGWMDMMAAFGDPGHEYKETITTGVAAAAAGGFTAVGLMPNTEPALHS
                     KSEIAYIVNKSKSSIVAIHPYGAITHNREGKELTEIYDMHHAGAVAFTDADVAIRDAG
                     IMLRSLQYVKPFKGVIINIPNDHKIVGNANVNEGVMSVQLGMYGIPDVLEELMVIRDI
                     KLAEYADSHVHFGVISSAKSLPHIREAKKRGIKVTAGVAAYQLFFDESMLFDYDTRLK
                     VNPPLRSKDDVAALIEGLADGTIDVICSYHLPHENDAKDVEFEYAAFGMESLEASFAA
                     ACKAIDGKLTITEMVEKMAINPRNILQLPIPSIKENNKAEFTIFNENTAWDFNESDIR
                     SRSHNTAFIGKSLKGKPLAIVNNNQLKQL"
     gene            1325..>1732
                     /locus_tag="PLB46_17015"
     CDS             1325..>1732
                     /locus_tag="PLB46_17015"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRF78283.1"
                     /translation="MEASAPELEQKNGSRKPIVTRNAIIISLILIGILLSAHFFAFGY
                     QNERIAFGQFVLVLVALIVTQLEVRDKLYQGILPFDKAFGTAMMVVLMVTAIFSVFTG
                     IFYLTIGTDVLTEMRTIAENSLREQGKTEEDIAS"
BASE COUNT          546 a          299 c          366 g          521 t
ORIGIN      
        1 atattccttg cggcggagat ccttttaatc cgttttttgc cgtagtaaaa atcaactatg
       61 cactgcatca ttcagggagt aaagattata gacccaactt caccttatca tctatctgtt
      121 aaatctattc gtattcataa tggtgtaatc gccgaaattg ctgatgttat aaacaatagc
      181 gatgcacagg tttacgatta taccggcaat tccgtttcac cgggttggat ggacatgatg
      241 gctgcatttg gcgacccggg tcatgaatac aaagaaacga taactacagg tgtagcggct
      301 gcagctgccg gtggatttac tgctgtagga ttaatgccaa ataccgaacc ggctttacat
      361 tccaaatcag aaattgccta catcgtaaat aaatcgaaat catcgattgt ggccatccat
      421 ccttatggtg ccataactca taaccgggaa ggaaaagaac tgaccgaaat ttatgatatg
      481 catcatgcag gtgcggtagc atttaccgat gccgatgttg ctattcgtga tgccggtatt
      541 atgctgagga gcttacaata tgtaaaacct ttcaaagggg ttattatcaa tatccctaac
      601 gaccataaaa ttgttggcaa tgcgaatgtg aatgaaggtg ttatgagtgt gcaattaggc
      661 atgtatggca tacctgatgt attggaagaa ttaatggtaa ttcgcgatat taaactggct
      721 gaatatgcag attcacatgt gcattttggt gtaatatctt cagcaaaatc gttaccgcat
      781 attcgcgaag caaaaaaacg tggaataaaa gtaactgcag gtgttgcagc atatcaattg
      841 ttttttgatg aatcgatgtt attcgattac gatacaagat taaaagttaa tccgccgttg
      901 cgttctaaag acgatgttgc ggctttaatt gaagggcttg cagatggcac tattgatgtg
      961 atttgcagtt atcatttacc gcatgaaaat gatgcaaaag atgttgaatt tgaatatgct
     1021 gcatttggta tggaaagttt agaagcttcg tttgctgcag catgtaaagc gatagatggc
     1081 aagttaacga ttacggaaat ggtagaaaaa atggccatta atcctagaaa tattttgcaa
     1141 ttacctattc catctattaa ggaaaacaat aaagcagaat tcaccatatt taatgaaaat
     1201 acggcatggg attttaatga atcagatatc cgttcacgct cacataatac cgcgtttatc
     1261 ggaaaatcat taaaaggtaa accattggca attgtaaata ataatcaact taaacaacta
     1321 taaattggaa gcgtcagcac cggaattaga acaaaaaaat gggtcgcgca agccaattgt
     1381 aaccagaaat gcgattatca tatcattgat attaatcggc attttattgt ccgcacattt
     1441 ttttgctttt gggtatcaaa acgaacgtat tgcattcggt cagtttgtat tggttttagt
     1501 tgcattgatt gtaacacaac tggaagtccg ggataaattg tatcagggta ttttaccttt
     1561 cgacaaagct tttggaaccg ccatgatggt agtgttaatg gttactgcca tattttcggt
     1621 ttttaccgga atattttatt taaccattgg gactgatgtg ctaacagaaa tgcgcaccat
     1681 agctgaaaat tcgcttcgtg agcagggcaa aaccgaggaa gacattgcta gc
//
LOCUS       DAORKW010000693         1726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4694_length_1726_cov_5.222621, whole genome shotgun sequence.
ACCESSION   DAORKW010000693 DAORKW010000000
VERSION     DAORKW010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4694_length_1726_cov_5.222621"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLB46_17020"
     CDS             <1..639
                     /locus_tag="PLB46_17020"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRF78284.1"
                     /translation="VMSIRLGDTAPDFIAQTTMGNIHFYDWAGSSWVVLFSHPADFTP
                     VCTTELGLAGRYKAAFSARNVKIIALSVDPIEKHMAWISDIETTQQTTIDYPLIADPE
                     KKIALLYDMIHPNASDSTTVRTVYVISPDKKVKLTIAYPASTGRNFDELLRVIDSLQL
                     TANFSVSTPANWKDGEDVIIIPSIQDEEIASKFPKGYHSVTPYLRITPQPNK"
     gene            645..>1726
                     /locus_tag="PLB46_17025"
     CDS             645..>1726
                     /locus_tag="PLB46_17025"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HRF78285.1"
                     /translation="MKRIVIIGGGLSGSLTALYLCQQKMDNFQIVIIEKSPSQLFKGL
                     AYASDLQVQPLNVPASGMSLYINEPNHFYNWLQANGPKAGIEQSDITPDYFAPRQLFG
                     TYLTENLNTQLQNCNAEIAIVHDEVINIQQEKDVYSISLDSGKTISCDKVVIASGNQD
                     NHFPYPITNSIYINGNVITNPWQPAVLNNIPNEAPILIVGCGLTMVDVLINLRKNGHK
                     GKLHIVSRHGLIPLPHKMGEHWDLQNKIPEKISVTTLIRYIKSEVKLAAAKNIGWQSV
                     MDAIRPYLPQWWNSLSLNDRIRLMEHMKPYWEIHRHRIPASTYAYLQNEIQSGGVAIY
                     GGKIKSLAANQNGIAAAIQLRKTSEN"
BASE COUNT          577 a          326 c          330 g          493 t
ORIGIN      
        1 gttatgagta ttcgattagg cgacaccgct cccgacttta ttgcccagac aactatgggt
       61 aatatccatt tttatgactg ggctggaagc agctgggttg ttttgttcag tcatcctgcc
      121 gatttcacac cggtatgtac tacagaacta ggcttggcag gtaggtacaa agcagctttc
      181 agtgccagga acgttaaaat tatagcactc agtgttgacc ctattgaaaa gcacatggcc
      241 tggatatcgg atattgaaac tacgcaacaa acaaccatcg attatccgct tatcgcagac
      301 cctgagaaaa aaattgcatt attatacgac atgattcatc caaatgccag tgacagcacc
      361 accgtcagaa ctgtgtatgt tattagtcct gacaaaaaag tgaaactcac catagcttac
      421 ccggcgtcta caggaagaaa tttcgatgag ttgttaagag tcatagacag cttgcaactg
      481 acagctaatt tcagtgtgtc aacccctgca aactggaaag atggtgaaga tgtaattatt
      541 attccttcca ttcaagatga agaaattgcc agcaaatttc caaaaggata tcacagtgtt
      601 actccttact tgcgtattac tccacaaccg aataaataaa tgatatgaag cgcattgtaa
      661 ttattggtgg cggactgagc gggtcattaa ctgcacttta tttatgccaa caaaaaatgg
      721 ataattttca aatagttatt attgaaaaat cgccatctca attatttaaa ggattggcat
      781 atgcttctga cttacaggtg cagccactaa atgttccggc ttctggcatg agtttgtata
      841 ttaatgaacc caaccatttt tacaattggt tacaagccaa tggcccaaag gcaggtattg
      901 agcaatctga tattacacct gattattttg caccacgtca gctattcgga acttatttaa
      961 ctgaaaacct taatacgcaa ctgcaaaatt gtaatgctga aattgcgatt gtacatgatg
     1021 aggtaattaa tattcaacag gaaaaagatg tatactcaat atcccttgat tccggcaaaa
     1081 caattagttg cgataaggtt gtaatagcaa gtggtaatca ggacaatcat tttccatatc
     1141 caataacaaa ttccatttat ataaacggca atgttattac taatccctgg caacctgcag
     1201 tactcaataa tataccgaat gaagctccaa tattaatagt tggttgtggt ttaacaatgg
     1261 tagatgtatt aattaattta agaaaaaatg gtcacaaggg aaaattacat attgtttcac
     1321 gtcatgggtt aataccatta ccacataaaa tgggtgaaca ttgggattta caaaacaaaa
     1381 tcccggaaaa aatttccgtg acaactttaa taagatatat aaagtcagag gtgaaattag
     1441 ctgctgccaa aaatataggt tggcaaagtg ttatggatgc tatcagacct tatttaccgc
     1501 aatggtggaa cagcctgagt ttaaacgacc gcatcagatt aatggagcat atgaagccct
     1561 attgggaaat tcaccgacac cgaatacctg catcaactta tgcctattta caaaatgaaa
     1621 ttcagtcggg cggagttgcc atttatggag gtaaaattaa gtcgcttgct gccaatcaaa
     1681 atggaattgc tgcagctatt caattgcgta agacttcgga aaatat
//
LOCUS       DAORKW010000694         1724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4704_length_1724_cov_3.587777, whole genome shotgun sequence.
ACCESSION   DAORKW010000694 DAORKW010000000
VERSION     DAORKW010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4704_length_1724_cov_3.587777"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLB46_17030"
     CDS             complement(<1..319)
                     /locus_tag="PLB46_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78286.1"
                     /translation="MLKHLTTIVLLLGLLQANAQVNIVITGTPIVENFNGFTGAGFAP
                     TPGAGQLDADNWRITGSNEGDLLFGGTKTTGDFARGTTGGNISTGGIYSLLTAAGANQ
                     VLYF"
     gene            470..>1724
                     /locus_tag="PLB46_17035"
     CDS             470..>1724
                     /locus_tag="PLB46_17035"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRF78287.1"
                     /translation="MYRLLTILLAASFLPLMAQEPGDDVFSTDQILKVELNFSQSGFW
                     DSLVANYSSETYMKADVTITDLTGVQVFEDIGIRLKGNSSYGHPGDKKSFKIDFNRYI
                     DSLDYDKLEKLNFNNCFKDPTFMREKIMYDLSHEVDVPAPRCIYANVYMNGTYWGFYD
                     VVEQIDDDFFSTHFDNSSENLFKAGAAFGAGTSAADLMYYGTDVADYEERYSLENNET
                     ENDWTDLISLTNFINNSSDADFADSLQYYFNVPVLMKSLVMDNMFANLDSYTNSARNY
                     YIYHNPVNGLWEWIKWDCNEAFGSYPIGMGGPPTDMTELATDYFALSRPLLERIVNTT
                     DTRATYDYYYCFIKNNYFSSSYLDPIIDALDDLIQSSVYADDNKMYTDAQYTDNLTEH
                     ITTGGGPGGGTIYGLKSFITERNKTG"
BASE COUNT          544 a          314 c          335 g          531 t
ORIGIN      
        1 gaaaataaag tacctgattg gcacctgctg cagttaataa cgaataaatt ccgccggttg
       61 aaatattacc tcctgtagtt cctctggcaa agtcgccggt agttttggtg ccaccgaata
      121 ataaatcgcc ttcgttactg ccggtaattc tccagttatc ggcatcaagc tgtcctgccc
      181 ccggcgttgg tgcaaatccg gctccggtaa atccattaaa attttcaaca attggtgttc
      241 cggttattac aatatttacc tgagcattcg cctgcagtaa acctagcaac aacacaatag
      301 tagtaaggtg tttgagcata atgtgtgttt taaataatga acaaagatat ccagtttggg
      361 ataaagctga aaaacagtta ccaaatttta taccacactt atcataatta atttttgtta
      421 atacgttaaa cctctgcatt tttgaccagt ctatcactaa aatcacgaca tgtatcgcct
      481 attaaccatt cttttagcag caagtttcct tccattaatg gctcaagagc cgggagacga
      541 tgttttttca accgatcaaa ttttaaaagt agaacttaac ttcagtcagt caggattttg
      601 ggatagcctg gtagcaaatt acagctccga aacctatatg aaagcagatg ttaccatcac
      661 cgatttaacc ggcgtgcagg tgtttgagga tattggtatt cgtttgaaag ggaattcctc
      721 ttatggccat ccgggagata aaaaatcatt taaaattgat tttaaccgat atatagattc
      781 attggattac gataaactgg aaaaactcaa ttttaataat tgttttaaag accccacatt
      841 tatgcgtgaa aaaatcatgt acgatttaag tcatgaagtt gatgtacctg cacccaggtg
      901 tatttacgca aatgtttata tgaatggcac ctattgggga ttttacgatg tagtagaaca
      961 aattgatgat gactttttca gtacacattt tgataatagt tcagaaaatt tatttaaagc
     1021 aggagccgca tttggagcag gtacgagtgc agcggattta atgtattacg gcaccgatgt
     1081 ggctgattac gaagaacgct attctttgga aaataatgag actgaaaatg actggactga
     1141 tttaatatcc ttaactaatt ttatcaataa cagttctgat gcagattttg cagattcgtt
     1201 acaatattat tttaatgtgc ccgtgctgat gaaaagtttg gtaatggata atatgtttgc
     1261 caacttagat tcctacacga attctgcaag aaattattat atctatcaca acccggtaaa
     1321 tggtttatgg gaatggataa aatgggattg caacgaggca tttggctcct accctattgg
     1381 tatgggaggc ccgccaaccg acatgactga attagcaaca gattattttg cattgagtcg
     1441 cccgttgttg gagcgtattg tgaatactac tgacaccaga gcaacttatg attactatta
     1501 ttgttttata aaaaataatt atttctcaag cagttacctc gacccgataa ttgatgcact
     1561 ggatgattta atacaaagca gcgtttatgc cgacgataat aaaatgtata ccgatgcaca
     1621 atacaccgac aacttaactg aacatatcac taccggtggt ggtccgggag gtggaaccat
     1681 ttatgggtta aaatctttta taactgaacg taataagacc ggac
//
LOCUS       DAORKW010000695         1722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4709_length_1722_cov_4.793041, whole genome shotgun sequence.
ACCESSION   DAORKW010000695 DAORKW010000000
VERSION     DAORKW010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4709_length_1722_cov_4.793041"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..>1722
                     /gene="lepA"
                     /locus_tag="PLB46_17040"
     CDS             170..>1722
                     /gene="lepA"
                     /locus_tag="PLB46_17040"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRF78288.1"
                     /translation="MEHIRNFCIIAHIDHGKSTLADRLLQITKTVSERDERAQVLDDM
                     DIERERGITIKSHAIQMDFNYEGKKYVLNLIDTPGHVDFSYEVSRSIAACEGALLLVD
                     ATQGIQAQTISNLYLAIEHNLEIIPIINKIDMDGAMIEEVKDQIVDLIGCERDTILEA
                     SGKTGLGIEAIFDAIIKRIPSPKGDPEAPLQALIFDSVFNSYRGVVAYYRIFQGTMKK
                     GERVMFVNTKNTYEANEVGIMRLLYEPRQEVKAGDVGYVITGIKEAKEIKVGDTITHK
                     EKPCVDIVEGFKDVKPMVFAGIYPIDNDDYEDLRDSLEKLQLNDASLVFEPESSVALG
                     FGFRCGFLGLLHLEIVQERLEREFDQNVIITVPNVSFDCFTTAGKKIVVHNPTDLPDP
                     GTIDHVEEPYIRAQIISQPDYIGAIMKLCMDKRGILKNQIYLTTTRVELVWEMPLAEI
                     VFDFYDKLKSISRGYASFDYELIDYRDGDLIKLDIKLNGENVDAFSAIIHRDKAYEFG
                     SKLCGKLKDL"
BASE COUNT          553 a          291 c          373 g          505 t
ORIGIN      
        1 ggttgattgc agcaaattgt agaaagtttc ccctgtcatc gaatgttaac ctgcagcaaa
       61 ttgttgcaat taaaaaatgt agaaattagg agctataacc acggtacatt tacgaagcac
      121 aaaggtttta gttacttttg catcctgaag aaaaagatag taacgtaaga tggaacatat
      181 caggaacttt tgcattattg cacatattga tcacggaaaa agtacgctag cagacagact
      241 tttacaaatt accaagactg tttctgagcg agatgaacgc gctcaggtgc tcgacgacat
      301 ggatattgag cgtgaaagag gcataaccat taagagccat gccattcaaa tggactttaa
      361 ttatgagggc aagaaatatg tgctcaattt aattgatact cccggtcacg tagactttag
      421 ttacgaggtt tcccgcagta ttgctgcctg tgaaggcgca ttattgttgg tagatgctac
      481 ccagggtatt caggcacaaa ccatatctaa cctttattta gccattgaac ataacctcga
      541 aattatccct atcatcaaca aaattgatat ggatggggcg atgatagagg aagtaaaaga
      601 ccagattgta gacttaatag gttgtgaacg cgatactatt ttagaggcca gtggaaaaac
      661 aggcttggga attgaagcca tttttgatgc cattatcaaa cgtattccgt ctccaaaagg
      721 cgacccggaa gcaccgttgc aagcgcttat tttcgattca gtgttcaact cctaccgtgg
      781 cgttgttgct tattaccgaa tttttcaggg tacgatgaaa aaaggagagc gcgtaatgtt
      841 tgtgaatacc aaaaacactt acgaagccaa tgaggttggt attatgcgtt tgttatatga
      901 accacgccag gaagtgaaag ccggcgatgt tggttatgta attaccggta tcaaagaagc
      961 caaagagatt aaggttgggg ataccataac ccataaggaa aaaccatgtg ttgatatagt
     1021 tgaaggattt aaagatgtaa aaccgatggt atttgccggt atttatccta tcgacaacga
     1081 cgattatgaa gatttaagag actcactgga aaaattacaa cttaatgatg catctcttgt
     1141 ttttgaaccg gaaagttcag tggctcttgg atttggattt cgctgtggat ttttagggtt
     1201 attgcatctt gaaattgtac aggaaagatt agaacgtgaa tttgaccaaa atgtaattat
     1261 tacagttcct aacgtttcat tcgactgctt tacaactgcc ggtaaaaaaa ttgtggtgca
     1321 caatcccacc gatttacctg acccgggaac aattgaccat gttgaggaac cctatattcg
     1381 tgcacaaata atttcgcaac ccgattatat cggagcaatt atgaaattgt gtatggataa
     1441 acgtggtatt ttgaaaaatc aaatttacct cactacaaca cgtgtggaat tagtttggga
     1501 aatgccactt gcagaaattg tattcgattt ttatgataaa ttaaaatcta tctctcgagg
     1561 atacgcgagt ttcgactatg aattaatcga ttatcgtgat ggagatttga ttaagttgga
     1621 tattaaactt aacggtgaaa atgttgatgc cttttcagcg attattcata gggataaggc
     1681 ttatgaattt ggcagcaaat tgtgcggtaa attaaaagac ct
//
LOCUS       DAORKW010000696         1720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4715_length_1720_cov_3.678078, whole genome shotgun sequence.
ACCESSION   DAORKW010000696 DAORKW010000000
VERSION     DAORKW010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4715_length_1720_cov_3.678078"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /gene="lpxK"
                     /locus_tag="PLB46_17045"
     CDS             complement(<1..617)
                     /gene="lpxK"
                     /locus_tag="PLB46_17045"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRF78289.1"
                     /translation="MRLLLLPLAWIYGLVIVIRNFFYNKGWLRSFEFDFPIILVGNLA
                     AGGTGKTPHVEYLIQLLQNNYKVATLSRGYGRSMKGYGLATELSLVEDIGDEPKGYKH
                     KFPATEVAVSENRVNGVYYLLNDEPDVKVIIMDDGFQHRRIKAGLNIVLTTWQKPYFK
                     DHMLPAGNLREPKAGVNRAQVVIVTKCPDDITQDQKMLYATKLGLS"
     gene            843..1178
                     /locus_tag="PLB46_17050"
     CDS             843..1178
                     /locus_tag="PLB46_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78290.1"
                     /translation="MKLFKKQGWIYVPVHGFGWLITGVLMVLAAGLFVGIDRHAHSAS
                     DTLIGYLPYGTSLAAIYFFIAINTSTNKNDNNERNNNESRNLRNKGGIDTYGGTLFIL
                     AWGCIGGWF"
     gene            1126..1467
                     /locus_tag="PLB46_17055"
     CDS             1126..1467
                     /locus_tag="PLB46_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78291.1"
                     /translation="MAVPSLFWLGVVLAVGFEKPVIVQEIMLPVDQISSLFTLMIMIG
                     LPTITFLVNLKAVMFIEFNPNSDNVFIDIHYRRNKSSWFLMVYAVLSIVVTISYAFFE
                     NFNFYSELLKP"
     gene            1464..>1720
                     /locus_tag="PLB46_17060"
     CDS             1464..>1720
                     /locus_tag="PLB46_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78292.1"
                     /translation="MMDKQLTFAVKVWGFATLVGSLLTCFALACVAVDPGIFIISLYL
                     IFFAITRGIPSLILFFIVVKLVSKTSWSLRSQLWVMALFGAV"
BASE COUNT          470 a          338 c          354 g          558 t
ORIGIN      
        1 gaaagcccga gtttagtggc atataacatc ttttgatcct gtgtaatgtc atcgggacat
       61 ttggtcacaa tcaccacctg tgcgcggttt acgcctgctt ttggctcacg taaattcccg
      121 gccggcagca tatggtcctt aaaataaggt ttttgccagg tggtaagcac aatattgagc
      181 cctgctttga tacgtcggtg ctgaaacccg tcgtccatga tgataacctt cacatcaggt
      241 tcgtcgttga gcaggtaata cacgccgttg acgcggtttt cgctgactgc cacttccgta
      301 gccggaaatt tatgtttgta ccctttgggc tcatctccaa tatcttccac taaactcagt
      361 tcggttgcca gtccatagcc tttcatagat ctgccgtagc cgcgacttaa agtggctact
      421 ttatagttgt tttgcagtaa ttgaatgagg tattcaacgt gaggagtttt gcctgttccg
      481 cctgcagcaa ggttgccaac taaaattatg gggaagtcga actcaaaact cctgagccaa
      541 cctttgttgt aaaaaaaatt cctgataact attactaaac cgtaaatcca ggcaagcggt
      601 aacaataaca aacgcatacg gggcgaaatt actaaaagta tggaacggaa gtcgaaaccc
      661 tttattggca agggttttgt ggcggccgcg tgcaaattac ctgccaaaaa cactgcctta
      721 agccacaagc tgctcccctg ctgtattccc ccggataaaa tattttgaaa aaaaatttgg
      781 tgaattgaca gaacaccgta actttgtatt tcaaagtaat ttgatttaga aacttatttg
      841 aaatgaaact ctttaaaaaa caaggttgga tatatgtccc agtgcacggt tttggctggt
      901 taattaccgg agtgcttatg gtattagccg ccgggctttt tgtgggtatt gaccgacatg
      961 cgcattcagc gagcgatacc ttgattggtt atttgccgta tggaacaagc ttagcggcca
     1021 tttatttttt tatagcaata aatacatcaa caaataaaaa tgataacaat gaaagaaaca
     1081 acaatgagtc ccgtaacctt cgcaacaagg gcgggattga tacttatggc ggtaccctct
     1141 ttattctggc ttggggttgt attggcggtt ggttttgaaa aaccggtaat cgtccaggaa
     1201 attatgttac ctgtagacca gatttcctca ttatttacat taatgattat gataggatta
     1261 cctaccatta catttttggt aaatctgaaa gcagttatgt ttatcgagtt taatccgaat
     1321 agtgataatg tttttatcga tatccattac agaagaaata aatcttcctg gtttttaatg
     1381 gtatatgcag tattgagtat tgtagtaacc atttcgtatg cttttttcga aaattttaat
     1441 ttttattccg aattacttaa accatgatgg ataaacaatt aacattcgca gtcaaagtat
     1501 ggggatttgc aacccttgtt ggcagtttat tgacctgttt tgctttagcc tgtgttgctg
     1561 ttgaccccgg gatttttatc atctccttat acttgatatt tttcgctatt accagaggta
     1621 ttccttcctt gatattattt tttattgttg ttaaactagt atccaaaaca tcatggtcac
     1681 tccgatctca actttgggta atggcgttgt ttggcgcggt
//
LOCUS       DAORKW010000697         1717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4730_length_1717_cov_4.383875, whole genome shotgun sequence.
ACCESSION   DAORKW010000697 DAORKW010000000
VERSION     DAORKW010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4730_length_1717_cov_4.383875"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLB46_17065"
     CDS             complement(<1..492)
                     /locus_tag="PLB46_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78293.1"
                     /translation="MRKRFLLSSLVAIALGFTSVNAQLYNYTNDLSGSPASVAANTSA
                     SSLGRVNGALASAGCADGFNSNEFSEGTIYATTKPAMEFSITPNSGYQLDVTSLSVDA
                     RHNNKGPVTWRLAYSTDGGATWVDNGADFNVGAISCGTSTTLSWDMADFSTYNTLYVR
                     VYAY"
     gene            896..1240
                     /locus_tag="PLB46_17070"
     CDS             896..1240
                     /locus_tag="PLB46_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRF78294.1"
                     /translation="MEVLIESPISLTESAVNEIKNIISEKQIPADYGLRVGVKGGGCS
                     GLSYILGFDLQKENDNVYQVGDIQIIMEKSHGMYLAGMEIDFVDGLNNRGFSFNNPNA
                     TKTCGCGTSFEA"
     gene            1335..>1717
                     /locus_tag="PLB46_17075"
     CDS             1335..>1717
                     /locus_tag="PLB46_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78295.1"
                     /translation="MKKLLTILLLVTSFAAKSQTFTDPIAYNDYIVDLQNKIGEKLIA
                     FNEQVASETASYASVEPYFLAMGAEMQDAVLKIRTLPDYEGNTDLRNAATALFDFYLN
                     TFQNEYLIMLNLVFDPDFDAEDLTKL"
BASE COUNT          573 a          275 c          334 g          535 t
ORIGIN      
        1 gtaggcataa actctcacat ataatgtatt ataggtagaa aaatctgcca tatcccaact
       61 taacgttgtt gaagtaccgc aagaaattgc accaacatta aaatctgcac cattgtcgac
      121 ccaagtagcg ccgccatcgg ttgaataagc taaacgccag gtaacagggc ctttattatt
      181 atgacgtgca tcaacagaaa gtgaggtaac gtctaactgg taacctgaat ttggtgtaat
      241 agagaactcc attgccggtt tagtggtggc ataaatggtt ccttctgaaa actcgttgga
      301 gttaaagcca tcggcgcaac ctgcagaggc gagcgcacca ttaactcttc ccaatgagga
      361 agcagaagtg tttgcggcaa ctgatgccgg tgacccggaa aggtcgtttg tgtaattgta
      421 caattgcgca ttgactgacg taaagccgag tgctatcgca accagggatg agagtaaaaa
      481 cctttttctc ataatgtgtg gtttaggtgt taaataaaat tgttatctgt gtctcaactc
      541 ttaggaatgg aaaggaaaga tgaaaatttt agttgatttt tcacaaagaa ctgcctgatt
      601 ctcaataata tggcttgtca caattttact atcacaactc attaatttta gttaattaca
      661 gggctttaca tctaaattat tataattatt tatccggttt tgccagtaac agggaatata
      721 cgtttgtagg tgattttggg ctaaaaagtc agttttaggg cgcacacggc gaagtcacaa
      781 cctgctcaaa atcatcaatt tgtgacaagc aaatcaatat gagcagactt attatgcttt
      841 gaaactttat ttaatggaat tgcgtttatt atttttgcat taaagcatta aaactatgga
      901 agtattaatc gagtcgccaa tcagccttac agaaagtgct gtaaacgaaa taaaaaatat
      961 tatcagcgaa aagcaaatac ctgccgacta cggtttacgc gttggtgtaa aaggaggtgg
     1021 atgtagtggt ttatcttata ttcttggatt tgatcttcaa aaagaaaacg ataatgttta
     1081 tcaggttgga gatatccaaa taataatgga aaaaagtcat ggcatgtatt tagccggaat
     1141 ggaaatagat ttcgtagatg gtttaaacaa cagaggtttt tcatttaata atccaaatgc
     1201 aactaaaacg tgtggttgcg gaacaagttt tgaagcctga catttattaa aataatattc
     1261 aacggcaatg gttactaaaa agtaagtgtt gccgtttttt tgtcgaaaca actaaaactt
     1321 tataaatatc aaatatgaaa aaactactta ccattctttt attagtaaca agttttgccg
     1381 ctaaaagcca aacatttact gacccaattg cttacaatga ttacattgtt gatcttcaaa
     1441 ataaaattgg agagaaatta attgcattta atgaacaggt tgcatcagaa actgcatcat
     1501 atgcaagtgt agaaccttat tttttggcaa tgggtgctga aatgcaggac gcagtattaa
     1561 aaattagaac tttacctgat tatgaaggta ataccgattt acgtaatgcg gctacggcat
     1621 tatttgattt ttatttaaat acttttcaaa acgaatattt aattatgttg aatttagttt
     1681 ttgaccctga ttttgatgcc gaagatttga ctaagct
//
LOCUS       DAORKW010000698         1714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4743_length_1714_cov_4.402049, whole genome shotgun sequence.
ACCESSION   DAORKW010000698 DAORKW010000000
VERSION     DAORKW010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4743_length_1714_cov_4.402049"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PLB46_17080"
     CDS             complement(<1..612)
                     /locus_tag="PLB46_17080"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRF78296.1"
                     /translation="MPNSPKSRYWLFFHIVFVYIASSFIWWSYLLHKNNVANFNDVVE
                     RERTQFAINGGLLSRFEQSSVYTKLHADFRRTELMLYGEGVVFIGLISLGFWRIRKTF
                     REEIAMTRQQNNFLLSITHELKSPLASLKLSMQTIQKRQLDAEHIKSMADMSMDDIER
                     LELLVENILLASKIESANFSLNRELMDLSTITNTIFDKIILKHT"
     gene            complement(605..>1714)
                     /locus_tag="PLB46_17085"
     CDS             complement(605..>1714)
                     /locus_tag="PLB46_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF78297.1"
                     /translation="TAGSGSLLIKLGNQVKAKGSNDYSLYGQEANGSTWALAKMNMFL
                     HGMDSAKIEWGDTIRSPKLLEKNKIMKFEGCYHGHSDALLVKAGSGLVTLGETSSSGV
                     PAAYANETIVVELDNEQAILNAIQQYENDIACIIIEPVPANNGLLLQRKEFLQFLKTC
                     CVENNILLIFDEVISGFRLGFEGAAGVYNIQPDIITFGKIIGGGLPVGAYGASKKIMA
                     MVSPDGGVYQAGTLSGNPLSISAGIAQLNACLQPGFYEDINRKTKLLADHINAYTFAH
                     KIPVNILTMGSVFWISFSENCVIRKAADIVPESLDKFKYFHAELLNRGVYIGPSGYEV
                     CFISSAHTENDINTASAIICEVLSLIFDANYIYHA"
BASE COUNT          561 a          343 c          280 g          530 t
ORIGIN      
        1 ggtatgtttg aggataattt tatcaaaaat agtatttgtg attgtagaca aatccattaa
       61 ttccctattt aaactaaaat tagcgctttc tatttttgat gccagcaata tattttcaac
      121 gagtaactca agtcgctcaa tatcatccat ggacatatcc gccatcgact taatgtgttc
      181 ggcatcgagt tgtcgttttt gaatggtttg catactcaat ttaagggagg ctagcggaga
      241 ttttaattca tgcgtaatgc tcaataaaaa attattttgt tgcctggtca tggcaatttc
      301 ttctctaaat gttttacgaa tacgccaaaa tcccaatgaa attaaaccga taaagacaac
      361 tccttctcca tataacatca actcagtacg tctgaaatcg gcgtgcaact tagtatacac
      421 cgagctctgt tcaaatcgcg acaataagcc tccgttaatg gcaaattgtg ttcgctcccg
      481 ctctactaca tcattgaaat tggctacatt gtttttatgc aataaatacg accaccaaat
      541 aaatgaactg gcaatgtata caaatacaat atgaaaaaaa agccaatatc tcgattttgg
      601 tgaattaggc atggtaaata taattagcat cgaaaattaa cgataaaact tcacaaatga
      661 ttgcacttgc agtgttaatg tcattttcgg tatgtgctga cgaaataaaa cacacttcat
      721 atccagaagg gccaatataa acacctctgt ttaacaattc agcatggaaa tacttaaatt
      781 tatctaacga ttcgggaaca atgtcagcag cttttctgat aacgcaattt tcagaaaatg
      841 aaatccagaa aacagagccc atggttaata tattaaccgg aattttatgg gcaaatgtat
      901 acgcattaat gtggtcggca agtaatttgg ttttgcgatt aatatcttcg taaaacccgg
      961 gttgcaaaca tgcatttagt tgagcaatcc cggcagaaat agatagtggg tttcctgata
     1021 atgtgcccgc ttggtaaaca ccaccatcag gtgaaaccat tgccataatt tttttgctcg
     1081 caccataagc accaacaggt aatccaccac cgatgatttt cccaaaagta atgatatcag
     1141 gctgtatatt atatactcct gctgcacctt caaagcctaa tctgaaacca ctgattactt
     1201 catcaaatat gagtaatata ttattttcaa cgcaacaggt ttttaaaaac tgaaggaatt
     1261 ctttgcgttg taataataaa ccattattag ctggaacagg ttcaattata atacaagcaa
     1321 tatcgttttc atattgttga atggcattta aaattgcctg ttcattatct aattccacca
     1381 caatagtttc attggcatac gcagcgggaa caccagatga tgaagtttca ccaagtgtaa
     1441 ccaaaccgga gcctgctttt acgagcaacg catcactatg cccgtgataa caaccttcaa
     1501 acttcataat tttatttttt tctaataact tggggctacg gattgtatcc ccccattcta
     1561 ttttggcact atccattccg tgtagaaaca tattcatctt agccaatgcc caagtagaac
     1621 catttgcttc ttgtccatac agagaataat cattagaacc ctttgcttta acttgattgc
     1681 ctaatttaat aagaagagaa ccggaacccg cagt
//
LOCUS       DAORKW010000699         1708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4785_length_1708_cov_3.596491, whole genome shotgun sequence.
ACCESSION   DAORKW010000699 DAORKW010000000
VERSION     DAORKW010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4785_length_1708_cov_3.596491"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLB46_17090"
     CDS             <1..390
                     /locus_tag="PLB46_17090"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRF78298.1"
                     /translation="IDNEGNVYLIGQHRYPFNEYTWEIPEGGGNKNEDPLVNIKRELR
                     EEAGLIADTWTLLQEIHVSNSVSDEYGYIYLAQNLTPCDPDPDDDEDLKILKIPFAEA
                     YQMLEDGKIKDSLTVIALLKAKIVLGL"
     gene            422..973
                     /locus_tag="PLB46_17095"
     CDS             422..973
                     /locus_tag="PLB46_17095"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRF78299.1"
                     /translation="MENNILDQEQTQYSEIKYAGFWERVGASLIDGLIFLPVYGLAFY
                     NTMNLKNLVLALVLGFVYLIYKIYMEGVKGATIGKRVMHIVVINDQNEKATMNQVIAR
                     NSLYILNTLVGIFSTVMIFNAAGFEDATTFMEISTFQQQNGSPIGTVVGVIILISVLV
                     VAFDKHKQALHDKIGKTYCAIRE"
     gene            complement(963..>1708)
                     /locus_tag="PLB46_17100"
     CDS             complement(963..>1708)
                     /locus_tag="PLB46_17100"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRF78300.1"
                     /translation="YHQLYSRYDILAIPNYGEFEGYPNRDSLAYRKIYGLENTATIVR
                     GTLRKRGFCDAWNVFVQLGMTDDTYSMENAHVFTWKMFTQAFLPGNATDVRQALADYL
                     GITDTVILDKLNWLGLFDETPVFENPDPTKLYSPAQILQHLLEQKWSLAAGDKDMCVM
                     MHVMDYTLDNVTRRIYSSMVATGKDEVYTAMAKTVGLPAAIATKMILTGAIKKTGVVI
                     PVTPDIYNPVLKELADKYAIQFVETTITL"
BASE COUNT          553 a          329 c          308 g          518 t
ORIGIN      
        1 atcgacaacg aaggaaatgt ttatcttatc ggtcagcacc gttatccctt taatgaatac
       61 acatgggaaa ttcctgaagg aggtggcaat aaaaatgaag acccattagt caatattaag
      121 cgggaattac gtgaagaagc agggttaatt gccgatacct ggacgctctt acaggaaatt
      181 catgtttcta attcggtgag cgacgaatac gggtatatct acctcgccca aaacctcacc
      241 ccttgcgacc ccgacccgga tgatgacgaa gatttaaaaa tcctgaaaat accatttgct
      301 gaagcctatc aaatgctgga agatggaaaa attaaggatt cattaacggt tattgctttg
      361 ttaaaagcta aaattgtact cggattgtag aaattaatta catttactca aaaataaata
      421 tatggaaaat aacatactcg accaggaaca aacccaatac agcgaaatta aatacgcagg
      481 attttgggag cgcgttggcg catcattaat tgatggttta atttttttac cggtatatgg
      541 attagctttt tataatacca tgaatcttaa aaatttagtc ctggcattgg tgttaggatt
      601 tgtatatctg atttacaaaa tttacatgga aggtgttaag ggtgcaacca taggtaaacg
      661 cgtaatgcat atcgtggtga ttaatgacca aaacgaaaaa gcaaccatga atcaggtaat
      721 tgcgcgaaat agtttatata tattaaatac gttggtaggt attttttcta ccgttatgat
      781 atttaatgca gccggatttg aagatgcgac cacgtttatg gaaataagca catttcagca
      841 acaaaatggc tcaccaattg ggactgttgt tggtgttatt attttaattt ctgtacttgt
      901 tgtagcattc gataaacaca aacaagcatt acacgataaa attggtaaaa cctattgcgc
      961 tatcagagag taattgttgt ttcaacaaac tgaatagcat atttatctgc taactctttt
     1021 aaaacaggat tgtaaatatc gggtgtaacc ggaattacaa ctcctgtttt tttaatggca
     1081 ccggttaaaa tcatttttgt cgcaattgca gccggcaatc ccaccgtttt tgccatcgct
     1141 gtgtatactt catctttccc tgtagccacc atgctggagt aaattctgcg cgttacatta
     1201 tccaatgtgt agtccatcac gtgcatcatc acacacatat ctttatcgcc tgctgccagc
     1261 gaccattttt gctctaataa atgttgtaat atttgtgcag gagaatataa tttcgttgga
     1321 tcaggatttt caaaaaccgg tgtttcatca aacaatccca accaatttaa tttatctaaa
     1381 ataaccgtat ctgtaatacc taaataatcg gcaagtgcct gacgaacatc agttgcatta
     1441 cccggtaaaa atgcctgcgt aaacattttc caagtaaata catgcgcatt ttccatgcta
     1501 taagtatcat ccgtcatacc caattgcaca aacacattcc acgcatcaca aaatccgcgt
     1561 ttgcgcaaag ttcctcttac aatggttgca gtattttcca atccgtaaat ttttctatac
     1621 gccaacgaat cgcgattcgg ataaccttca aactcaccat aattcggaat agctaaaata
     1681 tcgtaacgcg aatataattg gtggtaag
//
LOCUS       DAORKW010000700         1706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4791_length_1706_cov_4.980618, whole genome shotgun sequence.
ACCESSION   DAORKW010000700 DAORKW010000000
VERSION     DAORKW010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4791_length_1706_cov_4.980618"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /locus_tag="PLB46_17105"
     CDS             complement(<1..1667)
                     /locus_tag="PLB46_17105"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HRF78301.1"
                     /translation="MEINQASAYFGYTVSTAGDVNNDGYDDIIVGATLYDNGETDEGR
                     AYIYHGSPTGIIATPAKILECNQISAQFGSCVSDAGDINNDGFDDVIVGAQLYDNGQL
                     DEGVAFVYYGSPVGIKSTPFSLLESNQSVSRFGYAVSKAGDVNNDGYDDVVVGAYLAD
                     YGQDEEGAAFVYHGGPTGVNTVYAVKMESNQPYGRFGNSVSNAGDLNNDGYSDLIIGA
                     LQYNGIATNVGAAFIYHGSPTGIPSSPTTILAGTQGFAYLGSSVNTLGDVNGDGYSDV
                     IVGAYQYTNGQYDEGATYFYHGTSTGINPIPVLQLESNQSAAWFGYAVSGTGDINNDG
                     FDDAVVGAYLYDTPEAGGAAFVYLSSPCDLNFYADADGDGFGNNDEIIEACTAPPGYV
                     DNNLDCNDANNTINPTQIEICNVLDDNCNGINDEGVIETISIAAAGPTEFCQGGSVSL
                     TATYSGTSLQWQKNGTNIPGATLATYTVTTKGNYTCVTTSDCGTATSSTIFVNVFKNP
                     AATVSAAGPTTFCIGGSVTLNVTPVAGCSYQWYKDASPIPGATATNY"
BASE COUNT          509 a          383 c          298 g          516 t
ORIGIN      
        1 aaataatttg tcgctgttgc acccggaatt ggcgatgcat ctttatacca ttgataacta
       61 catcccgcaa ccggtgttac atttaaagtt acgcttcctc ctatacaaaa tgttgttgga
      121 ccagctgcgg agacagttgc tgcagggttt ttgaatacat ttacaaaaat agttgatgat
      181 gttgcagttc cgcaatcact ggtagttacg caagtgtagt tgccttttgt tgtaactgta
      241 taagttgcca atgttgcccc gggaatattg gttccatttt tttgccattg taaggatgta
      301 ccggagtagg tcgcagtgag tgacacgcta ccgccctggc aaaattctgt tggcccggca
      361 gcagcaatac taattgtttc aataacacct tcatcgttta ttccgttaca attatcatct
      421 aaaacattac aaatttctat ttgtgttgga ttaattgtat tattcgcatc attacaatca
      481 agattattat caacgtaacc cggaggagcg gtacatgctt caattatttc atcattattt
      541 ccgaaaccat cgccatctgc atctgcataa aaatttaaat cgcaaggact agataaatat
      601 acaaaggcgg cacctcctgc ttcaggtgta tcataaagat atgctccaac aacagcatca
      661 tcgaaaccat cattattaat atcaccggta ccggaaacgg cataaccaaa ccatgctgca
      721 ctttgattgg attccaattg caacacagga atcgggttta taccggttga tgttccatga
      781 taaaaataag tagccccttc atcatattgt ccattagtat attgatatgc gccaacaatt
      841 acatcggaat aaccatcacc atttacatca ccaagtgtat ttactgatga acccaaataa
      901 gcaaatcctt gagtgccggc caaaattgtt gttggactgc tcggaattcc tgttggtgaa
      961 ccatgataaa taaatgcagc tccaacatta gttgctatgc cgttatattg aagagctccg
     1021 ataattaaat cggaataacc atcattattc aaatcgcctg catttgatac actatttcca
     1081 aatctgccat aaggttggtt actttccatt tttaccgcat acactgtgtt tacacctgtt
     1141 ggaccgccat ggtaaacaaa tgctgcacct tcttcatctt gaccataatc cgcgagatat
     1201 gcgccaacaa ctacgtcgtc gtatccatcg ttattcacat caccggcttt tgaaacagcg
     1261 tatccaaaac gggaaaccga ttgattactt tcaagtaatg aaaagggcgt cgattttatg
     1321 ccaactggtg agccgtaata aacaaatgca acaccctcat caagttgacc attatcatat
     1381 aattgcgctc caacaattac atcatcaaat ccatcgttat taatgtcgcc tgcatcggaa
     1441 acacaactgc cgaattgtgc actgatttga ttacattcta aaatttttgc aggcgttgca
     1501 attatacctg ttggtgaacc atgataaata tatgctcgac cctcatccgt ttcaccatta
     1561 tcgtatagtg ttgcaccaac aataatgtcg tcatacccat cattgtttac atcacctgct
     1621 gtagaaaccg tataaccgaa ataagccgaa gcttgattta tttccaaaat atttgcaggc
     1681 gtattggata tgcctgttgc cgaacc
//
LOCUS       DAORKW010000701         1701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4821_length_1701_cov_5.131835, whole genome shotgun sequence.
ACCESSION   DAORKW010000701 DAORKW010000000
VERSION     DAORKW010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4821_length_1701_cov_5.131835"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1127)
                     /locus_tag="PLB46_17110"
     CDS             complement(105..1127)
                     /locus_tag="PLB46_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRF78302.1"
                     /translation="MSETIQLTAKDFATDQEVRWCPGCGDYSILKQVHTVLPQLGIPR
                     ENIVVVSGIGCSSRFPYYTNTFGMHSIHGRATAIASGLKAARPDLSVWIVTGDGDGLS
                     IGGNHMIHLLRRNFDVNVLLFNNEIYGLTKGQYSPTSPLGKVAKSTPMGSIDHPFNPA
                     ALALGADATFVARSMDRDPAHLQECLIRANAHKGTSFLEIYQNCNIFNDGAFEVFTEK
                     ANKKTNDLFVRHGQPLVFGESDNWGIKLDGFTPVAVDLNAGYTKDDLWVHDETDIYKA
                     QILARIFDNPTVEGHLPRPFGVFYATPKRATYEDLMNEQLNAALGKGEGDLDKLIRGS
                     ETWEIK"
     gene            complement(1287..>1701)
                     /locus_tag="PLB46_17115"
     CDS             complement(1287..>1701)
                     /locus_tag="PLB46_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HRF78303.1"
                     /translation="ISYDADNHEFMVKLREEKVERIADYIPLQTIESGPSKGKVLVVG
                     WGSTYGSIQSAVNHLLADGYEVAHAHIRYLKPFPKNLGEILFSYDKVLVPEINNGQLV
                     KVLRDKFMIPAIPYNKIKGTPITSGELIEAIMELM"
BASE COUNT          543 a          352 c          265 g          541 t
ORIGIN      
        1 attagcagaa aattaatatg tcacaaaaac gcctaatttt aacaaaaaaa taacccccaa
       61 cctgatggcc gggggttaaa cttattattt atgtgaggta aaccttattt aatttcccag
      121 gtttcagaac ctctgatgag tttatctaaa tcgccttcgc ctttacctaa agcagcattt
      181 aattgttcat tcattaaatc ttcgtaagta gcgcgttttg gtgtggcata aaatacacca
      241 aatgggcgag gtaaatgacc ttcaactgtt gggttgtcga aaatacgagc taaaatttgt
      301 gctttgtaaa tatccgtttc atcgtgcacc cacaaatcat ctttggtata acccgcattt
      361 aaatcaacag ctacaggagt aaatccatct aatttaatac cccagttatc tgattcgcca
      421 aatactaatg gctgaccatg acgaacaaat aaatcgttag tttttttgtt ggctttttca
      481 gtaaatactt caaatgcacc atcattaaag atgttacaat tttgatatat ttctaaaaac
      541 gaagtgccct tatgtgcatt tgcacgaatt aaacattcct gtaaatgagc agggtcgcga
      601 tccatacttc tggcaacaaa agttgcgtcg gcacctaatg ctaaagcagc aggattaaat
      661 ggatggtcaa tagaacccat tggagttgat ttcgcaactt ttcctaaagg tgatgttggt
      721 gaatattgtc cttttgttaa accataaatt tcgttgttga ataataatac attcacatca
      781 aaatttctgc gcaataagtg aatcatgtgg ttaccaccaa ttgataaacc gtcaccatct
      841 ccggtaacaa tccacacact taaatcaggg cgtgcagctt ttaaacctga tgcaatggca
      901 gttgcacgac cgtgaatact atgcatacca aatgtattgg tataataagg aaaacgcgaa
      961 gaacaaccga tacctgaaac caccacaata ttctcacgtg gaatacctaa ttgaggaagt
     1021 actgtatgca cttgtttcaa aatagaataa tcaccacaac cgggacacca cctaacttcc
     1081 tggtcagtcg caaaatcttt cgccgttaat tgaatcgttt cgctcataat atttttttat
     1141 ttttttccat tcaccgaagt aaatattttt aatttaattt ttttaatcaa tttgctgctg
     1201 ctattttaat gccttttaaa ccgctacatt tcataaccac ttcgaacccg gctgttaaat
     1261 cagcacatta acacatcagc acattatcac attagctcca taatcgcctc aatcaactcc
     1321 ccgctcgtaa ttggcgttcc tttaatttta ttatatggaa ttgcaggtat cataaattta
     1381 tctcttaata ccttcactaa ttgtccgtta ttaatttcag gaaccaatac tttatcgtag
     1441 ctgaataaaa tttcacctaa atttttaggg aaaggtttca ggtaacgaat atgtgcgtga
     1501 gccacttcat aaccatcggc taataaatgg ttaactgcac tttgaattga gccataagtt
     1561 gaaccccatc caacaactaa aacttttcct ttcgaaggac cactttcaat agtttgtaat
     1621 ggaatataat cagcaatacg ttccactttt tcttcgcgca atttcaccat aaattcatgg
     1681 ttatcggcat cgtaagaaat g
//
LOCUS       DAORKW010000702         1693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4864_length_1693_cov_4.769841, whole genome shotgun sequence.
ACCESSION   DAORKW010000702 DAORKW010000000
VERSION     DAORKW010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4864_length_1693_cov_4.769841"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLB46_17120"
     CDS             complement(<1..753)
                     /locus_tag="PLB46_17120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HRF78304.1"
                     /translation="MSEPIDEILVDNHRNAANAELDKIQSFTGKYPKQLWYLFTVEMW
                     ERFCFYGMRGMLTVFMASQLGLTDKAANLQYGAIQAFVYAFTFLGGLFADKILGFRKS
                     LFWGALMMIAGGFIIAAAPEQFFYIGICFSIIGTGFFKPNISTMVGQLYKDGDPRRDA
                     GFSLFYSGINLGALLGGILMVWVGLQVSWSLAFGLVGVVMSISIVTFWFTQKTLGPIG
                     LSPLSITMPASKKRMYEIATYIGATPECSNEVS"
     gene            complement(788..>1693)
                     /locus_tag="PLB46_17125"
     CDS             complement(788..>1693)
                     /locus_tag="PLB46_17125"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRF78305.1"
                     /translation="KEDRRPIAALLSVYACIVIFWAVFHQNGDALTVWAEEYSHRKMS
                     DGVANVAEDIGMAQTVNYHPSVEYNEEEFSSYLAGLGTTRDSLTAAGNEEGAKAISDE
                     IAIRTASHRYFENLPEENRPAPGGDLKLVSTELYQSINPFWVVLLTPLVVGVFGFLRK
                     KGKEPTTPTKIAIGLVITALSALVMVLAVKSVDIANDKASSLWLIATYGVITIGELCL
                     SPMGLSLVSKLSPPRLTALMMGGFFLSTSVGNKLAGILSSNFEATDDKGGFFFTNFVL
                     VMLAAALLFALLKWLNGVMKEKGVN"
BASE COUNT          537 a          366 c          287 g          503 t
ORIGIN      
        1 ggacacttca ttactacatt caggcgtcgc tccaatataa gtggctattt cgtacatccg
       61 tttttttgat gccggcatcg taatgctgag tggtgataaa ccgattggtc ccaacgtttt
      121 ttgtgtaaac caaaacgtta caatactaat tgacattacc acaccaacca atccgaatgc
      181 gaggctccat gaaacttgta aaccaaccca aaccattaga ataccaccta ataatgcacc
      241 taagttgata cctgaataaa aaagactgaa accagcatct ctgcgtgggt caccatcttt
      301 atacaactgg cctaccatgg tggagatatt cggtttgaaa aagcccgttc ctattatgga
      361 gaaacaaata ccgatataaa aaaattgttc aggtgctgct gcgatgataa atccaccggc
      421 aatcatcatt aaagctcccc aaaaaagtga ttttctaaat cccaatattt tgtcggcaaa
      481 taaaccacct aaaaatgtaa atgcgtaaac aaatgcctga atggcgccat attgtaaatt
      541 ggcggcttta tcggttaatc ctaactgact tgccataaat acggtgagca tcccgcgcat
      601 gccataaaaa caaaagcgct cccacatttc aacggtaaat aaataccata attgttttgg
      661 gtatttacct gtaaacgact gaattttatc taattcagca tttgcagcat ttcgatgatt
      721 gtcaactaat atttcatcta ttggttcact cataatgatt tttttatttg aattggtaaa
      781 tatgaattta attcactcct ttttctttca tcaccccatt tagccatttt aataatgcaa
      841 acaacaatgc agcagcgagc attactaaaa caaaattggt gaagaaaaat ccgcctttat
      901 catctgttgc ttcaaagttg ctggataaaa taccggctaa tttgttaccc acagaggtag
      961 ataagaaaaa tccacccatc atcaatgccg ttaaacgcgg aggtgataat ttacttacta
     1021 atgacaatcc cattggggat aaacacaatt caccgattgt gataacacca taagtggcaa
     1081 ttaaccataa actgcttgct ttatcgtttg caatgtcgac agatttaact gcgagcacca
     1141 taaccaatgc acttaatgca gtaattacga gaccaatagc aattttggtt ggtgttgtag
     1201 gttctttgcc ttttttgcga aggaagccaa acacacctac tactaatggt gttaacaata
     1261 caacccagaa tggatttatc gattgatata attctgtaga aactaatttt aaatcaccgc
     1321 cgggagcagg acgattttct tcaggtaaat tttcaaaata acgatgagaa gctgttctga
     1381 tggctatttc atcgcttatc gcttttgcgc cttcttcatt tccggcagca gtaagcgagt
     1441 cgcgtgtcgt gcctaaaccg gctaaataac tgctgaattc ttcttcatta tactccactg
     1501 aaggatgata atttaccgtt tgtgccattc caatatcttc agcaacattg gcaacgccat
     1561 cactcatttt gcggtgagaa tattcttctg cccatacggt aagtgcatca ccattttgat
     1621 ggaataccgc ccaaaaaata acaatacagg catatacact taataacgct gcaattggtc
     1681 tgcggtcttc ttt
//
LOCUS       DAORKW010000703         1691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4875_length_1691_cov_5.556846, whole genome shotgun sequence.
ACCESSION   DAORKW010000703 DAORKW010000000
VERSION     DAORKW010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4875_length_1691_cov_5.556846"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PLB46_17130"
     CDS             <1..867
                     /locus_tag="PLB46_17130"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HRF78306.1"
                     /translation="DCDDTSPFNYPFAVDFCDGLDNDCDMIIDEDIVEPLIAVEGADD
                     ICTGGLTHLVAKPVYPGYQIQWYRNGFPVADANHTTFDVSKTGNYYVKYTAPAGCITT
                     STTKVVLVATPPSPIVYNVSASNDLCKNNPVKLQVKNKIGNTYQWYKGVLPIAGATSN
                     IYFATTPGFYKVLQSDVNGCAGISKPFRVVNFCDEGMFTAQTSSIQLFPNPNNGNFVL
                     ACNFNNAYTGNAKLLMHNMIGDIVWESEIAVTQGEVNAQINTNNQLPNGMYLVTISVG
                     QWQNNMQFILAH"
     gene            complement(965..>1691)
                     /locus_tag="PLB46_17135"
     CDS             complement(965..>1691)
                     /locus_tag="PLB46_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HRF78307.1"
                     /translation="LHVHIGMPSRDVAVFMMNSLRYFLPHIFALSTNSPFWEGRDTGF
                     KSFRTKVFDKFPRTGIPDVFNDASHFDNFIALLVKTNCIDNGKKIWWDLRVHPYFNTI
                     EFRMCDIPLRVDETLALAALMQAITAKLFKLMKSNIGWRNYDRALIQENKWRAARYGI
                     SGKLIDFGKQEELDTTTLLMEFLDFVDDVVDELGSRQELENIKWMLKNGTGADRQLAH
                     YYANDRNFNSLVDFMIKESYIGL"
BASE COUNT          552 a          302 c          289 g          548 t
ORIGIN      
        1 gattgtgatg atacatctcc atttaattat ccatttgctg tagacttttg tgatggatta
       61 gataacgatt gtgatatgat aattgatgaa gatattgttg aaccacttat agctgtagaa
      121 ggtgctgatg acatttgcac aggaggttta acacacttag tcgctaaacc tgtttatccc
      181 ggttatcaaa ttcaatggta tagaaatgga ttcccggtgg cagatgccaa ccacaccaca
      241 tttgatgtgt ccaaaactgg aaattattat gtaaaatata cagcaccggc agggtgtatt
      301 actacctcta ctacaaaagt agtactggtg gctacaccgc caagtcctat tgtatacaat
      361 gtctcagcca gcaatgattt atgcaaaaat aatcctgtaa aactgcaggt gaaaaataaa
      421 attggtaata cctatcagtg gtataaaggt gttttgccaa tagccggagc tacttcgaac
      481 atatattttg ctacaacacc cggcttttat aaagtattac aatctgatgt taatggttgt
      541 gcaggtattt ctaaaccatt tagagttgtg aatttttgcg atgaaggtat gtttacagca
      601 caaacttcta gtatacaatt atttcctaat ccaaataatg gcaactttgt attggcatgc
      661 aattttaata atgcctatac tggaaacgca aaattgttaa tgcacaatat gataggtgat
      721 attgtgtggg aatcggaaat tgcagtaacc caaggtgagg tgaatgcaca aattaatacc
      781 aacaatcaat taccaaatgg catgtattta gttaccattt cagttgggca atggcaaaat
      841 aatatgcaat ttattttagc gcattaaagc gttgggatat attttttaat tgattaaaat
      901 ttacaacaaa caataaaaaa aggcatccat ttaagatgcc ttttaagttg gtgtaaatat
      961 gttgttataa accaatataa ctttctttta tcataaaatc taccagcgaa ttaaagttgc
     1021 ggtcgtttgc ataataatgc gctaattgcc tgtccgcacc ggttccattt ttcaacatcc
     1081 atttaatgtt ttctaattct tgtctgctgc ctaattcatc cacaacatca tcaacaaaat
     1141 ctaaaaattc cattaatagg gtagtagtat ctaattcttc ctgtttgccg aagtcaatta
     1201 atttaccact tataccgtaa cgagcagcac gccatttatt ttcctgaatt aaagcgcggt
     1261 cgtaatttct ccaaccgata ttacttttca tcagtttaaa taattttgcc gtaatcgctt
     1321 gcattaatgc agccaacgcc aatgtttcat caacgcgtaa tggaatatca cacatcctga
     1381 attcaatggt gttaaagtat ggatgcactc gtaaatccca ccatattttt ttgccattat
     1441 cgatacaatt tgtttttacc aataatgcaa tgaagttgtc gaagtgactg gcatcattaa
     1501 atacatcggg aatacccgtt cttggaaatt tgtcgaatac ttttgttctg aatgatttaa
     1561 aaccggtatc tcttccttcc caaaaaggtg aattagtact taatgcaaaa atatgtggta
     1621 agaaataacg caatgagttc atcataaaaa cagccacatc acgcgatggc atgccaatgt
     1681 gaacatgtaa t
//
LOCUS       DAORKW010000704         1690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4880_length_1690_cov_5.253823, whole genome shotgun sequence.
ACCESSION   DAORKW010000704 DAORKW010000000
VERSION     DAORKW010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4880_length_1690_cov_5.253823"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1690
                     /locus_tag="PLB46_17140"
     CDS             <1..>1690
                     /locus_tag="PLB46_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78308.1"
                     /translation="LLAGYSDSDSSAQKTEDAIGPGYNVFDEDDWVTYHYEYTDIWLV
                     KLDADGNLEWENTIGGDRDDFVSAMVETTDGGYIIAANSESDISGDKTEDCWAPYLNS
                     TDYWIIKLDADGTIMWQNTIGGEYKDWVNSIKQTADGGYILGGLSRSPISGDKTEAAF
                     CGGEDLWIVKLNASGTIQWQNAIGGCATDRVNSIEQTVDGGYIVGATSYSGIYGDKTE
                     ICYGNSDFWILKLSAIGTLIWQNTIGGNHYDDLSVIRQLPDGNFIVGGNSGSDISFDK
                     TEANIGSNDFWILKLNSTGSIIWQNTIGGDDSESLENILPTSDGGYIAIGSSRSGISG
                     DKTIENFEFYNSADYWVLKLDAFGNITWQSAYGGNADDYPKNLMLTDDGGFMLSGYSY
                     SPISGNKTNASLGEEDYWAIKLSPECFASPEICNTFDDNCNGIIDDDVIETITISAAG
                     PTEFCQGGNVILNASYSGTSVQWQKNGVDIPGATLASYTATTKGNYSCVTTSDCATSI
                     SETIFVNVFKNPKAIISAAGPTTFCVGGNVTLNVSPVAGCSYQWYKDASPIPGAT"
BASE COUNT          539 a          265 c          372 g          514 t
ORIGIN      
        1 atttattagc tggatattcc gattctgaca gctcagcaca aaaaactgag gatgccatag
       61 gccccggcta caatgttttt gatgaagatg attgggtgac ttaccattat gagtatacag
      121 atatttggtt ggtgaaattg gatgctgatg gcaacttgga atgggaaaat accattggtg
      181 gtgatagaga tgactttgta agtgcaatgg tggagactac agatggcggt tacataattg
      241 cagccaattc ggaatctgat atttccggtg ataaaaccga ggattgttgg gcaccatatt
      301 taaattctac cgattattgg ataattaaat tagatgctga tggaaccatt atgtggcaaa
      361 atactattgg cggggaatat aaagattggg taaattcaat aaaacaaacc gcagacggag
      421 ggtatatatt aggaggttta tcacgttcgc caatttccgg tgataaaacc gaagccgcat
      481 tttgtggtgg tgaagacctt tggatagtta aattaaatgc atctggaaca attcaatggc
      541 aaaatgctat tgggggttgt gccaccgaca gagtgaatag tatagagcaa accgttgatg
      601 gcggatatat tgttggtgcc acttcctatt cgggaattta tggtgataaa acagaaattt
      661 gttatggcaa ttccgatttt tggatactta aattaagtgc aataggtact ttaatatggc
      721 aaaatacaat tggaggaaat cactatgacg acttaagcgt aattcgtcaa ttacctgatg
      781 gcaattttat tgtaggcgga aattcaggat cagatatttc tttcgataaa acggaagcaa
      841 atattggaag taatgatttt tggatattaa aactgaattc aacgggctca atcatttggc
      901 aaaatacaat tggtggcgat gattctgaat cattggaaaa tatattacca acatctgatg
      961 gtggttatat tgcgataggt tcatccagat ctggaatttc aggtgataaa acaattgaaa
     1021 atttcgaatt ttataattcc gctgattact gggtgcttaa attagatgca ttcggcaata
     1081 tcacatggca atctgcttat ggtggtaatg ctgatgacta ccccaaaaac ctgatgctga
     1141 ctgatgatgg aggatttatg ctttccggtt attcctattc acctatttca ggaaataaaa
     1201 caaatgcatc attaggggaa gaagattatt gggctataaa attatctccc gaatgttttg
     1261 cttcacctga aatttgtaac acgtttgatg ataattgcaa cggcataatt gatgatgatg
     1321 taattgaaac aattaccatt tctgctgccg gtccaactga attttgtcag ggtggaaatg
     1381 taatattaaa tgcaagctat tcaggaacat ctgtccaatg gcaaaaaaat ggtgttgata
     1441 ttcccggagc tacattagca tcatacaccg caactacaaa aggtaattat tcttgtgtaa
     1501 ctaccagcga ttgtgcaaca tcaatttccg agacaatatt tgtaaacgta tttaaaaatc
     1561 caaaagcaat aatttccgca gccggtccaa ctacgttttg tgttggtgga aatgttactt
     1621 taaatgtcag tccggttgcg ggatgcagct atcaatggta taaagatgca tcgccaattc
     1681 cgggtgcaac
//
LOCUS       DAORKW010000705         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4884_length_1689_cov_5.217870, whole genome shotgun sequence.
ACCESSION   DAORKW010000705 DAORKW010000000
VERSION     DAORKW010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4884_length_1689_cov_5.217870"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..>1689
                     /locus_tag="PLB46_17145"
     CDS             349..>1689
                     /locus_tag="PLB46_17145"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF78309.1"
                     /translation="MDTKKQRAVKTKLLQSIQRAYQIALGSKQKGIPLQEYHQSLQIN
                     RRQFLATTGKATLFTLAAGSSFITACSTANKPTIAIVGAGIAGLNAAYTLKRAGFIAQ
                     IYEASDRTGGRIITATDLMGKGLTTEMGGEFIDSTHADMLLLAQTFNLPLLDTFVPSE
                     SNLIHACYYINNKVITDKDILELFYPYTEKLSADIDSISDEISYSKHSENDVRLDHMS
                     IAQYLQSIGITGDLYKLLDVAYTTEYGCDIEQQSAINFLMLMNPEMHDTFNYSGFSDE
                     RYKIKGGNQLITDALEKRLETQLNKGYKLEAIRRSDINGQTMLTFATEGGVKEVVADF
                     VILALPFTVLREIKMDCGFNKPKLKAINEMGYGKNAKFFHGFSKAHWRDLGYTGFTFT
                     DTNLQNGWDNSQLQQGEGAGFTIFTGGKAGEVLNQLSDAEIATQLDPVMENCFPG"
BASE COUNT          565 a          270 c          344 g          510 t
ORIGIN      
        1 aattgtaaag cttgcaatca tattaaaaaa gaaacttata ccatagctaa actcaccaaa
       61 tcaagttcat caggttcagg tggtagtgga ggcggaggtg gcggtagttc atggggcggt
      121 ggtagctccg gaggcggagg tggcggcagc tcatggtaat tattgcattt gtttaaaatg
      181 cggttattca tctgtaatta attacagcag gtataacatg tttttgtgca atacattaat
      241 tgagcaattg cataattcaa ccatcatagt tgaatgaaaa catgtgaaaa tgctaaaatt
      301 ttgtttcacc aataagttta tttacattta ctgtatgaaa ctttttgtat ggatacgaag
      361 aaacagcgtg ccgttaagac aaagctattg caatcaattc aacgagctta tcaaatagcg
      421 cttggtagta agcaaaaagg aataccactt caagaatatc accagtcatt acaaatcaat
      481 cgccgccaat ttcttgcaac aacaggaaaa gccacattat ttacattggc tgcaggttct
      541 tcatttatta cggcatgttc cacagcaaat aaacctacta tagccattgt aggtgctgga
      601 attgcaggct taaatgcagc atatacatta aaacgtgcag gttttattgc acagatttat
      661 gaagcatcag acagaactgg tggtagaata attaccgcaa ctgatttaat gggcaaagga
      721 ttaactaccg aaatgggggg tgaatttatt gattcaacgc atgcagatat gttattattg
      781 gcacaaactt ttaatctccc gttattagat acattcgtgc catcggaaag taatttaatt
      841 catgcttgtt attatattaa taataaagtt attaccgata aagacattct cgaattattt
      901 tatccttata cagaaaaatt gtctgctgat attgatagta ttagtgatga aatatcatat
      961 agtaaacatt cggaaaatga tgtgcggtta gaccatatgt ccattgcgca atatttacaa
     1021 tcaataggta ttacaggcga tttgtataaa ctgttggatg tggcttatac aacagaatac
     1081 ggttgtgata tagaacaaca atctgcaatt aattttttaa tgctgatgaa tcctgaaatg
     1141 cacgatacat ttaattattc gggatttagt gatgaacgat ataaaattaa gggtggtaat
     1201 cagttaatta ccgatgcatt ggaaaaacga ttggaaactc agttgaataa aggatacaaa
     1261 ctggaagcaa ttcgacgcag cgatatcaat ggacaaacca tgttaacctt cgcaactgaa
     1321 ggtggtgtaa aagaagttgt cgccgatttt gtaattcttg ctttgccttt taccgttttg
     1381 cgcgaaataa aaatggattg cggatttaat aaacccaaat taaaagcaat taacgaaatg
     1441 ggttatggaa aaaatgccaa gttttttcat ggcttttcaa aagctcactg gcgcgattta
     1501 ggatataccg gttttacgtt tactgatacc aatttgcaaa atggttggga caatagtcag
     1561 ctccaacaag gtgaaggtgc agggtttacc atttttaccg gaggaaaggc aggcgaagtg
     1621 ctcaatcaat tgagcgatgc tgaaattgca acccaattgg atcctgtaat ggaaaattgt
     1681 tttcccggc
//
LOCUS       DAORKW010000706         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4885_length_1689_cov_4.309058, whole genome shotgun sequence.
ACCESSION   DAORKW010000706 DAORKW010000000
VERSION     DAORKW010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4885_length_1689_cov_4.309058"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLB46_17150"
     CDS             <1..230
                     /locus_tag="PLB46_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78310.1"
                     /translation="LGIDEDYLFICDGNGGLRMYSINVPTSPTLLQVVDIEESFDVIT
                     ANGLLIVVTADGYHLFDYSSGTLNEVGAIKK"
     gene            complement(361..747)
                     /locus_tag="PLB46_17155"
     CDS             complement(361..747)
                     /locus_tag="PLB46_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRF78311.1"
                     /translation="MNKVTGIGGIFFKCKDPNKMKDWYNTNLGLKTDQYGATFEWLQS
                     DDPTKKGYTQWSTFAEDTKYFQPSTKEFMINYRVENLEALVEELRNNGVTIIDSISSF
                     DYGKFIHILDPEGNAIELWEPTDYQF"
     gene            complement(933..1358)
                     /locus_tag="PLB46_17160"
     CDS             complement(933..1358)
                     /locus_tag="PLB46_17160"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HRF78312.1"
                     /translation="MPAKKSKLATIKTQETTASVEQFLNTIEDEQKRKDSYVIVEMMK
                     KATKAEPKMWGSAIIGFGNTRYVSPKTGREVDWLIIGFSPRKANLSLYVKIHANNNPD
                     LIKKLGKCKTGAGCLYINKLSDIDAGILQEIINASVKTK"
     gene            complement(1441..>1689)
                     /locus_tag="PLB46_17165"
     CDS             complement(1441..>1689)
                     /locus_tag="PLB46_17165"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HRF78313.1"
                     /translation="PYLMPDFPQKYAKAQIENAKEAGKSEAEVAQVEEEMQKYLESIE
                     NPLIYGLMTFAEPFPVGLIITLISSMILKRKTNIGATD"
BASE COUNT          481 a          278 c          288 g          642 t
ORIGIN      
        1 gattaggtat agatgaagat tatttattta tctgtgatgg caatggcgga ttacgcatgt
       61 attcgataaa tgtgcctacg tcacctacat tattacaagt tgtagatatt gaagaaagtt
      121 ttgatgtaat caccgcaaac ggattactaa tagtagtgac tgctgatggt tatcatttat
      181 ttgattatag cagtggtacc ttaaatgagg ttggagcaat taaaaaataa aagtgtaaca
      241 attacactaa aatcataata aaaattgggt gcaagagtta tttacttttg cacccaattt
      301 tttatgctgt aactttagga aaggcagcaa aaaactttgt tgttatgatt gttttcccat
      361 ttaaaattga taatctgttg gctcccagag ctcaatggca ttgccttccg ggtcgaggat
      421 atgtataaat ttaccgtagt cgaaactgga tatactatca ataatggtta caccattatt
      481 tcttaattct tcaaccaatg cttccagatt ttcgaccctg taattaatca taaactcttt
      541 tgttgatggc tggaagtatt ttgtatcctc ggcaaatgta ctccattgtg tatatccctt
      601 tttggttgga tcatcacttt gcaaccattc gaaagttgca ccgtattggt ctgtttttaa
      661 tcccaggttg gtattgtacc aatctttcat tttgttaggg tctttacatt taaaaaagat
      721 gccacctata ccggttactt tgttcatttt aataagcttt ttaagttgta atttataatg
      781 tgaatcgttt tgatgcaata tgaaaatata acaattgttg tagttgcctt ataaataagt
      841 tataggtaac ctcaatttct gtttgttcaa atataagtaa tttgccaaaa ggtcgctgat
      901 tatgtgatta atcggatgct tgttctagtt agttacttcg tttttacgct agcgttgata
      961 atttcctgaa gtatgccggc atctatatcg ctaagtttat taatatataa acatcctgca
     1021 cctgttttac atttgcctaa tttttttatt aaatccggat tgttgttagc atgtattttt
     1081 acataaagcg aaaggttggc tttacgtggt gaaaacccaa ttattagcca atcgacttct
     1141 cttccggttt taggactgac atatctcgta ttgccgaaac caataattgc actgccccac
     1201 atcttgggct ctgctttggt tgccttcttc atcatttcca ctataacata actatccttt
     1261 cgtttttgtt catcttcaat tgtatttaga aattgttcta cgctggctgt tgtttcttgc
     1321 gtttttatag tagctaattt tgattttttt gcaggcatat caaggtgttt aatgtaaatt
     1381 aatgatataa ttatatcaaa accaaacgag gttaattatc ccaatttaaa tgacttaata
     1441 ctaatcggtt gccccaatat ttgtttttcg ttttaatatc atggaggaaa taagggtaat
     1501 aattaatcct acaggaaaag gctcagcaaa ggtcattaac ccatatatta atggattttc
     1561 aatggattcc agatattttt gcatttcttc ttctacctgt gcaacttctg cctctgactt
     1621 gcctgcttct ttagcatttt caatttgtgc tttggcatat ttttgcggaa aatcaggcat
     1681 cagataagg
//
LOCUS       DAORKW010000707         1688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4891_length_1688_cov_5.082058, whole genome shotgun sequence.
ACCESSION   DAORKW010000707 DAORKW010000000
VERSION     DAORKW010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4891_length_1688_cov_5.082058"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /locus_tag="PLB46_17170"
     CDS             complement(<1..1679)
                     /locus_tag="PLB46_17170"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRF78314.1"
                     /translation="MEIAIVLALLVLAIIAFSTEKISVDIVTMTAVLLLVLTGILDYK
                     EAFAPFGSDFIIMLASIFIVTSAIDSSGVLEHWTSKLMRRSGGSQMKLIMPMITITSA
                     FSAFMNNTTVTALMINPAMAIAKKSGINFSKILLPLAFASIVGGTCTLIGTSTNVAVN
                     AYLSKNGYEAFGMWDFTIIGVIMVIVTLLYMATVGVRMLPNRHNGDLTAEYGVREYLS
                     EIKILAGSTLIGQVIHDSEISRMGFNVLAVIRGDRQFAPGPNISFREGDIVLVKGAMQ
                     QLLQVKNTKGIDIVADTLDFYQPNDKALKLTEVVVPGRSDLAGSTIAEIDFRRKYGMV
                     IVAINREGKNITEQLNNIVIEVGDMLLVQGTDDAFNKLKNHHDLVVLSAHIPPVKNLW
                     KGYFALGFFCLAIIISSIKFFNVHVSVAFLASAVLSMLVGAVKPDDAYKSIEWKLLVL
                     IAGMTAFGTAMGNSGADKYLAELIVSWFGGFGPTGIMLGFMVLTVLLTQPMSNAAAAL
                     VVLPVALEAANTLHVNPLSYAIAVMLSASVSLITPFEPSCILVYGPGKYKF"
BASE COUNT          550 a          367 c          282 g          489 t
ORIGIN      
        1 ataaatttat atttacccgg cccatatact aaaatacatg atggctcaaa tggagttatt
       61 aaagaaactg atgcagaaag cattacagca attgcataac ttaatggatt aacatgcaag
      121 gtatttgccg cttccaatgc tacaggcaat actactaatg ccgcagccgc gttgctcata
      181 ggttgagtaa gtaacactgt taataccata aaacccaaca tgataccggt aggtccaaaa
      241 ccaccaaacc agctgactat taattctgct aaatatttat cggcgcctga attacccatt
      301 gcggttccga atgctgtcat acccgcaata agcaccaata atttccattc tatacttttg
      361 taggcatcat ccggtttaac tgcaccaacc aacatactta aaactgcgct tgctaaaaac
      421 gcaacagaaa cgtgcacgtt aaaaaattta atagagctta taattattgc caaacagaag
      481 aagcccaatg cgaaataccc tttccacaaa tttttaaccg gtggaatatg tgcagataat
      541 acaaccaggt cgtggtgatt ttttaattta ttaaaagcat catcagtacc ttgcacaagc
      601 aacatatcac caacttcaat aacaatattg tttaattgct ctgtaatatt ttttccttca
      661 cggttaatag ccactatcac cataccgtat tttcttctaa aatcgatttc ggcaattgtt
      721 gaaccggcta aatcagaacg tcccggcaca accacttcgg ttaatttaag tgccttatca
      781 tttggttgat aaaaatctaa agtatccgca acaatatcaa ttccttttgt attttttact
      841 tgcagtaatt gttgcattgc accttttact aaaacaatat ccccttcacg gaaagaaata
      901 ttaggtccgg gggcaaattg tctgtctcct cttattacag ccagaacatt aaaacccatg
      961 cggcttattt ctgagtcgtg aattacctga ccaattaaag ttgaaccggc taatatttta
     1021 atttcgctca aatattctct tacgccatat tctgcagtga ggtcaccatt gtgtctgttc
     1081 ggtaacattc ttacaccaac tgttgccatg tacaacagtg taacaattac cataataaca
     1141 ccaattatcg taaagtccca catgccaaat gcttcatagc catttttgct caaataggca
     1201 ttcacagcca cgtttgtaga agtaccaatt aaagtacaag ttcctcctac aattgaggca
     1261 aaagcgaggg gcagtaatat tttggaaaag ttaattcccg attttttggc aatagccatt
     1321 gcagggttaa tcattaaagc cgttaccgta gtgttattca taaaagccga aaaagcactg
     1381 gtaatagtaa tcataggcat tatcagtttc atttggcttc ctcccgaacg gcgcatgagt
     1441 ttggaagtcc aatgttccaa cacccctgag gaatcaattg ccgaagtgac aataaaaatg
     1501 gatgccaaca tgatgataaa atcgctgccg aaaggggcga acgcttcctt gtagtcgaga
     1561 attcctgtca aaaccagcag caatactgcg gtcatcgtga caatatctac cgatattttt
     1621 tcggtagaaa aggcaataat agccaatact agcagggcta gtacaattgc aatttccata
     1681 agagtaat
//
LOCUS       DAORKW010000708         1678 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4941_length_1678_cov_3.584104, whole genome shotgun sequence.
ACCESSION   DAORKW010000708 DAORKW010000000
VERSION     DAORKW010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4941_length_1678_cov_3.584104"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..1625
                     /locus_tag="PLB46_17175"
     CDS             339..1625
                     /locus_tag="PLB46_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HRF78315.1"
                     /translation="MSFNKSQASMTFIFITLLIDVIGFGIIIPVLPSLIKELHGGDFS
                     EAARIGGWLLFAFAIMQFIFSPILGNLSDRYGRRPVLLLALFGFGCDYLIMAFAPDLI
                     WLFVGRILAGITGASFSTASAYIADVSPPEKRAQNFGLIGAAFGMGFIIGPAVGGLLG
                     ELGSRVPFFAAAGLSFVNWLYGFFVMPESLPKEKRRPFEWKRANPIGSLLALKKYPVT
                     YGLIASLVCIYIASHAVQSNWSYFTMGTLGWSEKAVGLSLAFVGLLVGIVQGGLIRIT
                     IPRFGQKNSLYYGLALYIIGLFLMGIASQSWMMYVFLIPYCLGGIGMPALQGIISSQV
                     PSNEQGELQGAMTGLMSLTSIVGPPLMTNTYAYFTADSAPVHFAGAAFILGAVFMLVS
                     LVLARNTLKHQDIPNHVKAEVEPVIQNIHDNDPSIL"
BASE COUNT          465 a          265 c          385 g          563 t
ORIGIN      
        1 aatgtgagaa tgtaccgatg taccaatttg gggaatgtaa ttatgtgtga atgtagcgtt
       61 ataccaacgc gcgaattgta agccgcggcg gatactgatg agctaattgg gtggtacaga
      121 gagcagttaa ttattacgga aaaaaccaag gatgcgctaa gcatccgcaa aagtggaaat
      181 gatagcacga aacaagcaag gtagtcgaaa ccccatcagc taatttgcta atcagctaat
      241 ttactaattt caggttgttt cctaaaaatc ccggagcatt cagttaattt tgaccaaatt
      301 ttttttagcc agtaaaatca cttaattagt ttacaaaaat gtcatttaac aaatcacaag
      361 cttcaatgac ctttatattt atcacgttgc tgatagatgt aattggtttt ggcattatta
      421 taccggtgtt gccgagttta attaaagaat tacacggggg agattttagt gaggctgcgc
      481 gaattggtgg atggctctta tttgcatttg ccattatgca atttattttt tcacctattt
      541 taggaaattt aagtgacagg tatggtcgca ggcctgtttt attattggca ttatttggtt
      601 tcggttgtga ttatttaatc atggcttttg ctccggattt aatttggttg tttgtcgggc
      661 gaattcttgc aggtattacc ggtgcaagtt tttccactgc aagtgcttat attgctgatg
      721 tttctcctcc ggaaaaaaga gcgcaaaatt tcggattaat tggagccgca tttggaatgg
      781 gatttattat tggtcccgca gttggtggtt tgctcggtga gttgggttca agagtgccat
      841 tttttgcagc agcaggtttg tcgtttgtga attggttata cggatttttt gtaatgcctg
      901 aatcattacc aaaagaaaaa cgcagaccat ttgaatggaa acgtgcaaat cctattggtt
      961 cattattggc tttaaaaaaa tatccggtta cgtatggatt aattgcctca ttggtttgta
     1021 tttatattgc ttcacatgca gttcaaagta attggagtta ttttaccatg ggcactttag
     1081 gatggagcga aaaagcagtt ggtttgtcac ttgcttttgt tggtttattg gtcggcatag
     1141 tacaaggtgg attaattcgg ataaccattc caaggttcgg acaaaaaaac tcattgtatt
     1201 atggtttagc attatatatc ataggattat ttttaatggg cattgccagc caaagctgga
     1261 tgatgtatgt atttttaata ccttattgtt taggtggaat cggaatgccg gcattacagg
     1321 gaattatttc ttcgcaagta ccaagtaatg aacaagggga gttgcaaggg gcgatgacag
     1381 gtttgatgag tttaacatct atagttggac cgccattaat gacaaatacg tatgcctatt
     1441 ttaccgcaga tagtgcaccg gtgcattttg caggcgctgc atttatttta ggtgcggtat
     1501 ttatgctggt aagtttagta ttagcacgta atacgctcaa acatcaagac attcccaatc
     1561 atgtaaaagc agaggtggag cctgttattc aaaatattca tgataacgac ccaagtattc
     1621 tatgagtttc tcatgttgag cttcgcaggt ggcttgtccg cctaattgca gggggact
//
LOCUS       DAORKW010000709         1676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_4949_length_1676_cov_4.423812, whole genome shotgun sequence.
ACCESSION   DAORKW010000709 DAORKW010000000
VERSION     DAORKW010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4949_length_1676_cov_4.423812"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..>1676)
                     /locus_tag="PLB46_17180"
     CDS             complement(277..>1676)
                     /locus_tag="PLB46_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HRF78316.1"
                     /translation="NYMVDFEMECHKLGIPLRTRNNEVAPGQFECAPMFEEASIAVDH
                     NCLLMDVMEKVARHHKLRVLFHEKPFAGVNGSGKHNNWSMGTDTGKNLLSPGKTPRTN
                     LQFLTFFINTIKAVHDNADLLRASIASANNDHRLGANEAPPAIISAFIGTALRSVLNE
                     IENRVDAKTFDDLDKADLRLDIHNKIPDLLLDNTDRNRTSPFAFTGNKFEFRAVGSSA
                     NCSKAMTTVNTIVAAQLKAFREEVDGLIKGGENKDNAILKVLTRYIGESKNILFEGDN
                     YSEEWAKEATENRGLNNVKTTPEAIDFYATERAKNIFVKNHILSERELHARMEIEFEE
                     YAKKIQIEARIYGELVYNYVLPAAVEYQNALLDNVMKLKAVGLNEAHYKEQIDLATNI
                     TLHLNHLRAALERMVEERKRANRIEHAREKAFAYCNNVKTLFDEIREHADMLERYLPD
                     TKWPLAKYRELLFVK"
BASE COUNT          495 a          316 c          290 g          575 t
ORIGIN      
        1 atacacataa tgccaaaagt aatttgtttg tcggtttcat tgtatttgag tgttttgaca
       61 aaggtgccaa gcttaggttg cactgacaat ccaatttttt gtaaaactta gaaacctcat
      121 aaatggcatt attgacacga gtgcgaaaca ctcattcaat ttttggaaga catgatgaca
      181 aaggcaattg catataacaa caactccgac attaaaaaaa atgcaaaaaa aagaccctgc
      241 cgaaacaagg tcttgaaacc aatttatcaa tactaattat tttacaaata acaattcacg
      301 atatttagct aatggccatt tggtatccgg taaataacgt tccaacatat cggcatgttc
      361 gcgaatttca tcgaataatg tttttacatt attacaatag gcaaatgctt tttcgcgtgc
      421 atgttcaata cgatttgcgc gtttacgttc ttccaccata cgctctaatg cagcgcgtaa
      481 atgatttaaa tgcaaagtaa tgttggtagc taaatctatt tgttctttgt aatgtgcttc
      541 gtttaatcct acagctttta atttcataac attgtctaac aatgcatttt gatattcgac
      601 agcggcaggt aaaacatagt tgtaaactaa ttctccataa atacgcgctt caatctgaat
      661 ttttttcgca tattcttcga attcaatttc catacgagcg tgtaattcgc gttcgctaag
      721 gatatgattt tttacgaaaa tattttttgc gcgttctgtt gcataaaagt cgatagcctc
      781 aggtgtagtt tttacattat ttaaaccacg attttcggta gcttcttttg cccactcttc
      841 actgtagtta tcaccttcaa acaaaatatt tttgctttcg ccgatgtaac gggttaatac
      901 ttttaaaatg gcgttgtctt tgttttcacc acctttaatt aaaccgtcca cttcttcgcg
      961 gaatgctttt aattgtgcgg caacaatggt atttacggtt gtcattgcct tactgcaatt
     1021 ggcacttgaa cctactgcgc ggaattcgaa tttattaccg gtaaatgcaa acggactggt
     1081 acggttacgg tcggtattat ctaataataa atccggaatt ttattgtgaa tgtctaaacg
     1141 taaatcagct ttatctaaat cgtcgaatgt tttagcatca acacgatttt cgatttcatt
     1201 taatactgaa cgtaaagctg ttccaataaa agcagaaata atggctggtg gcgcttcgtt
     1261 ggcacctaaa cgatgatcgt tgttggcgct tgcaatactg gcacgtaata aatcagcatt
     1321 gtcgtgcacg gctttaatgg tattaataaa gaaggttaaa aattgtaagt tggttcttgg
     1381 tgttttaccc ggacttaata aattttttcc ggtgtcagta cccatactcc agttattgtg
     1441 tttacctgaa ccgtttacac ctgcaaatgg tttttcgtgg aataatacac gtaatttatg
     1501 atgacgtgct actttttcca ttacatccat taacaaacaa ttgtggtcaa ctgcaatact
     1561 tgcttcttca aacatcggag cacattcaaa ttgtcccggt gcaacttcat tgttacgtgt
     1621 gcggagtggt atacctaatt tatgacattc catttcgaag tccaccatgt aattaa
//
LOCUS       DAORKW010000710         1665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5006_length_1665_cov_3.453416, whole genome shotgun sequence.
ACCESSION   DAORKW010000710 DAORKW010000000
VERSION     DAORKW010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5006_length_1665_cov_3.453416"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /gene="cphA"
                     /locus_tag="PLB46_17185"
     CDS             complement(<1..1510)
                     /gene="cphA"
                     /locus_tag="PLB46_17185"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HRF78317.1"
                     /translation="MKILSTAVMKGPNYWSIRRHKLIVMKLDLEEMEDMPTNKIPNFR
                     ENLEKMIPSLYEHRCSEDHAGGFFKRVEEGTWMGHVVEHVALELQTLAGLDTGFGRTR
                     STNTHGVYNVVYSYLEEKTGLYAGQVAVEFCQALASGADYDITPHIQRMKEIREQERL
                     GPSTGSIVEEAIKRDIPYIRLNRRSLVQLGWGINQRRIQATIASTTSNIAVEIACDKE
                     ETKNLLGQLNIPTPRGGSAYDTDDLEAIVRRIGYPIVIKPIDGNHGRGSTMNIKNWDE
                     AVAALKKAKEISRWSIVERYVEGYDFRLLVVNYKFVSAALRKPAAVMGDGQHTIKELI
                     DHVNSDPRRGYGHENVLTSIKLDDHTLRLLEKLGMDENSVPRNGEEIFLKSTANLSTG
                     GTATDVTDYIHPYNIFTAERIARVIGLDICGIDIMSKDLSVPMTENGGVVLEVNAAPG
                     FRMHLAPSEGIGRNVAEPVVDMLFPAGSKGRIPIVAVTGTNGKTTTTRLNAHI"
BASE COUNT          473 a          378 c          279 g          535 t
ORIGIN      
        1 caatgtgagc atttaaacgc gtagtagttg ttttaccatt tgttccggta acagcaacaa
       61 tcggaattct gccctttgaa cctgcaggaa ataacatatc tacaaccggt tcagcaacgt
      121 tgcgacctat accttcgctt ggtgccaagt gcatgcggaa acccggagca gcatttactt
      181 ccagcaccac accaccgttt tcggtcatag gtactgaaag gtctttactc ataatatcaa
      241 taccacaaat atctaatcca attacacgtg caattctttc tgcagtaaaa atattatatg
      301 gatgaatata atctgttaca tctgttgccg ttccacccgt agataaattc gcagtcgatt
      361 ttaaaaatat ttcttcacca tttctcggaa cggaattttc atccatacct aatttttcta
      421 ataaacgtaa tgtatgatca tctaatttaa ttgatgtcaa tacattttca tgtccgtacc
      481 cgcgacgcgg atcactattt acatgatcaa ttaattcttt aatggtatgt tgtccatcac
      541 ccattaccgc agcaggtttt cttaatgccg cagaaacgaa tttataattt acaaccagta
      601 atctgaaatc atatccttcc acataacgtt caacaatact ccagcgtgaa atttcttttg
      661 ctttttttaa tgcagcaact gcttcatccc aatttttaat attcatggtt gaaccgcgac
      721 catgattacc atcaataggt tttattacaa tcggatagcc tattctgcgc acaatcgctt
      781 ccaaatcatc agtatcataa gcagaaccac cgcgcggtgt aggaatattt aactgaccta
      841 ataaattttt tgtttcttct ttatcgcagg caatttctac agcaatattt gaagtagtac
      901 tcgcaatagt agcctgaatt ctgcgctgat ttattcccca tcctaactgc accaacgaac
      961 gacgatttaa acggatgtaa ggaatgtcac gtttaatagc ttcctcaaca atacttcctg
     1021 ttgatggccc taaacgttcc tgctcacgaa tttctttcat gcgctgaata tggggagtga
     1081 tatcataatc ggctccgctt gccaatgcct gacaaaattc tacagctact tgtcccgcat
     1141 ataaaccggt tttttcttcg agataagaat acaccacatt ataaacacca tgtgtatttg
     1201 tgcttctggt tctgccgaat cctgtatcga gtccggcaag ggtttgtaat tcgagtgcaa
     1261 catgttctac aacgtgtccc atccaggttc cttcttccac gcgtttaaaa aatccgcctg
     1321 catggtcttc actacagcga tgttcgtaaa gagaaggaat cattttttct aaattctcac
     1381 gaaaattcgg aatcttgttc gtgggcatgt cttccatttc ctccaaatca agtttcatta
     1441 caattaactt atgcctgcga atactccagt agttcgggcc cttcatcact gcggtagata
     1501 ggattttcat ttttcgatgt tctttgcgct aaaaataagg aaatttagta aaagaacgcg
     1561 gatgtcgctg atgacgctga ttttcctgcc tggcggcaaa tgaagctatt gtagtttttt
     1621 tatccacaat acgaaacctt agtgcaacta agggtttatt caaat
//
LOCUS       DAORKW010000711         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5174_length_1632_cov_4.339252, whole genome shotgun sequence.
ACCESSION   DAORKW010000711 DAORKW010000000
VERSION     DAORKW010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5174_length_1632_cov_4.339252"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1182)
                     /locus_tag="PLB46_17190"
     CDS             complement(208..1182)
                     /locus_tag="PLB46_17190"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRF78318.1"
                     /translation="MSLCGISKHQYYHKGKNGKRGVKPSESTVKNTDAGEEQLLLKNE
                     IVVNEIVQIKSNKDTDYGYQKMTAALALLGYCINHKKVYRIMKEQGLLNSKTRCTGKT
                     RVRFRQVESKTPLEVLEIDIKYKRISGSGRMAYIMTIIDTFTREVLYWTCGYSMKQVQ
                     VKEAWEYVIIHYLQESDTLKKGLKVEVRNDNGSQFEAKMVREFLEENGITQVFTHPYT
                     PEENGHIESFHAILNNSIGNEEYETLQELESRLKNFYNTYNNIRLHSSICNLPPQVFK
                     LLWEDKLVKMEKGKKYKTKFKLLIPYYQINKYVSTKKRKKNDHRAMRA"
     gene            complement(1224..1541)
                     /locus_tag="PLB46_17195"
     CDS             complement(1224..1541)
                     /locus_tag="PLB46_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRF78319.1"
                     /translation="MEKQQKPTEKEKLAILKEAELHGVTATLNKYGIYPATYYYWKRK
                     VSEAGNEGLKHGMTKERLQEINRLKRENDLLKKIIAEKELESKLKDELLAKKILAWKK
                     EKR"
BASE COUNT          383 a          344 c          239 g          666 t
ORIGIN      
        1 atttatctct aggatagaaa taaaaatctt tactttttgg tctaaccccc tattaatcgg
       61 acaatattca aagttaatta atacttgtga catttattca taattttgct tctgagacga
      121 tagcgaccga tggtgatctt tataccgaag tcgttttttg ttagtgttgg gcccaaccta
      181 actttcttag cggagacgat agctccctta ggctctcatt gcccgatggt catttttttt
      241 gcgcttcttt gtcgatacat atttattgat ttgataatac ggaattagca gtttgaattt
      301 tgttttatac ttcttgccct tttccatttt tactagctta tcctcccaaa gcaatttaaa
      361 aacctgtggt ggtagattgc aaattgaact atgcagcctg atgttgttat aggtgttata
      421 aaaattcttt aatctgcttt ccagctcttg taatgtctca tattcttcat ttcctattga
      481 attgtttaat atcgcatgga agctttctat gtgaccattt tcctctggcg tgtatggatg
      541 ggtaaacact tgagttattc cattttcttc caaaaattct cgcaccatct ttgcctcgaa
      601 ttggcttcca ttatcattcc ttacttctac ttttaaccct tttttaagcg tatctgattc
      661 ttgcaaataa tggataatca catactccca tgcttctttt acctgcactt gtttcattga
      721 atagccgcat gtccaatata atacctctct tgtaaatgta tcgattattg tcattatata
      781 agccattctc cccgacccgc ttattctttt gtatttaata tctatttcta atacttctaa
      841 aggcgttttg ctttctactt gcctaaagcg tacgcgggtc ttacctgtac aacgtgtctt
      901 tgaatttaga agcccttgtt ccttcataat tcggtacacc tttttgtgat ttatacaata
      961 gcctaaaagt gccaaagctg cggtcatttt ttgatacccg taatcagtgt ccttattgct
     1021 cttaatttgt acaatctcat tcaccactat ttcatttttt aacaacagtt gttcttcccc
     1081 agcatcagta tttttaactg tcgattcgct tggttttact cctctttttc cattttttcc
     1141 tttatgataa tactgatgtt tgctaattcc gcaaagactg attgcagttc ctacagatag
     1201 accctggaag ttgtaggacc ttactatcgc ttttctttct tccaggccaa tatctttttt
     1261 gccaataact catcctttaa tttgctctct aattcttttt cagctataat ttttttgagc
     1321 aagtcatttt cacgcttaag ccggttgatt tcctgcagtc tttcctttgt cattccatgc
     1381 ttaagtcctt catttccagc ttcacttacc tttcttttcc aataatagta ggtagcagga
     1441 tatatgccgt atttgttcag tgtggctgtt acaccgtgta actcagcttc tttcagaata
     1501 gccaatttct ctttttctgt gggtttttgt tgtttttcca tttccaatat tacagttttt
     1561 ttatcgtaat attgtccgat tatttaaggg gctgaactac ttttcaaagg tttgccaaca
     1621 attttaccct ca
//
LOCUS       DAORKW010000712         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5177_length_1632_cov_3.211795, whole genome shotgun sequence.
ACCESSION   DAORKW010000712 DAORKW010000000
VERSION     DAORKW010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5177_length_1632_cov_3.211795"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PLB46_17200"
     CDS             complement(<1..1008)
                     /locus_tag="PLB46_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78320.1"
                     /translation="MRTIIVNNDTLTIDTLSIIPGTLTISYVEAGDTLFTTLDSSFYK
                     VEYFNSRLIFLKPYQGVIRIQYQVFPYLFTRTYFNKNPALFDSANINFKPYVIDIKNS
                     EPYLNISGLDYNGSFARGISFGNNQDVVVNSDFNLQLNGKLQNDVNISASITDNNIPI
                     QPEGNTQQLQEFDKVFIQLSKNRQKLIMGDYEIYRPPGYFMNYYKKLQGASYTGAFDL
                     YGGTFTSGLSLAVAKGNYSRQDIPIIEGNQGPYKLKGNNGETFIIILAGTERVYIDGK
                     LMVRGAENDYIIDYNAGEIAFTTNVLLTKDKRVQIEFEYSDKNYFRSLVYSGSTFTSG
                     DD"
     gene            complement(1163..>1632)
                     /locus_tag="PLB46_17205"
     CDS             complement(1163..>1632)
                     /locus_tag="PLB46_17205"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRF78321.1"
                     /translation="KTKQIYSDINSDKLPVDDKFMKNLPMWRSFDRFVSSYGIRIAWG
                     VAYFLFYLAFATHWWMWLLFPITCLMGPVHGAIINYFAHKYGYRNFEMEDTSKNFLPV
                     DVLMMGESYHNNHHKLLARPNFGVRWFEIDPTYPIIRLLNLMHVIRLNKEPVS"
BASE COUNT          518 a          310 c          280 g          524 t
ORIGIN      
        1 atcatctcct gatgtaaaag tgcttcccga atacactaat gaacggaaat aatttttatc
       61 ggaatattca aactcgattt gtaccctttt atcttttgtc aataatacat ttgttgtgaa
      121 tgcaatttcg ccggcattat aatcaataat ataatcgttc tctgcaccac gaaccattaa
      181 ttttccatca atataaacac gttcagtgcc tgctaaaata ataatgaatg tttcaccatt
      241 atttcccttc aacttatatg gtccttgatt accttctatt atcggaatgt cttgccttga
      301 ataattaccc ttggcaactg ctaaactcaa accactggta aatgtgcctc cataaaggtc
      361 aaacgctccg gtgtaactag ctccctgtaa ttttttataa taattcataa aataacccgg
      421 aggtcgataa atttcgtaat cgcccatgat gagtttttgc ctgtttttcg acaactggat
      481 aaatactttg tcaaattcct gcaattgttg tgtattccct tccggttgaa tggggatatt
      541 attatctgtt attgatgcag atatattgac atcattttgc aacttgccgt ttaactgcaa
      601 attaaaatca gaattcacca ccacatcctg attattgcca aatgaaattc ctcttgcaaa
      661 actgccatta taatctaatc cggaaatatt gagatatggt tctgaatttt ttatatcaat
      721 tacataaggt ttgaagttga tatttgcact atcgaacaac gccggatttt tattaaaata
      781 tgtgcgcgta aataaataag gaaatacctg atattgaata cgaataacgc cttggtaggg
      841 tttgaggaag attaatcgtg aattaaaata ttcaacctta taaaaactac tatccaaagt
      901 tgtaaataac gtatctcctg cttcaacata agaaattgtc aatgtaccgg gaataatact
      961 aagtgtatca atggttagcg tgtcgttgtt gactattatt gtgcgcactc gttcattacc
     1021 cgactgtgca tttatatgcg cagtggtaaa taagatgata caaactgata tcaggagtcg
     1081 cagtctaata gcctgtcttt ttttggtaaa gttaattgta agacaggaaa cgttgctgaa
     1141 gcgtggtatg gtatcccgtt tactaggaaa ccggttcttt gtttaaacga attacatgca
     1201 tcaaattgag taaacgaata atagggtagg tggggtctat ttcaaaccaa cgaaccccga
     1261 aatttggacg agcaagtaac ttatggtgat tattatggta gctttcaccc atcataagca
     1321 catcaaccgg taaaaagttt ttactggtgt cttccatctc aaagttccga tatccgtatt
     1381 tatgtgcaaa atagttaatt atagctccgt gaacaggccc cattaaacac gtaatgggaa
     1441 acagtaacca catccaccag tgtgtggcaa aagccagata aaacaagaaa tatgctacac
     1501 cccaggcaat gcggatacca taagaactga caaaccggtc gaaactacgc cacatgggga
     1561 ggtttttcat gaatttatca tctaccggca atttatcgct gttaatatca ctataaatct
     1621 gtttggtttt cc
//
LOCUS       DAORKW010000713         1628 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5199_length_1628_cov_4.291163, whole genome shotgun sequence.
ACCESSION   DAORKW010000713 DAORKW010000000
VERSION     DAORKW010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5199_length_1628_cov_4.291163"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1628)
                     /locus_tag="PLB46_17210"
     CDS             complement(<1..>1628)
                     /locus_tag="PLB46_17210"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HRF78322.1"
                     /translation="QDSSWLIVEHPPLSKKDDGLDAPIAFASKIEVEKYLQQYLESYQ
                     QDGYLTATWNFTKQNADTLYATIVAGKKVYWATLNTANVDEFALAGVGYRKKDINGKV
                     FNHQEVVGMMQKMLTYSESVGYPFATIQLDSIIWGDSVISADLIYRKNQFIVFDTLLV
                     VGNLNVSANYLIQYTGITPGAPFNQQLLNTLENRLKEIPFATKSKNTKIEFSGNRAKI
                     IMYLDEKQSSTFDFLIGVIPNEAITGRLIITGEGRLQLHNVFNAGELFNFHFSKLEST
                     SKELQTSLVYPYLPKLPIGLEGAFSLYLKDSTFLERTTTAGVLFQMTGNNYFKALANF
                     YNSAVLAIDTGYILSNLQLPSSLDMVERAYGLEWNFEKLNYRFNPYKGYALKIGATIG
                     NKTIKENASIVELQDPLNPEFDFSTLYDSIDLKSLSVKYHYQLGWYIPLFSRVTLLLQ
                     TRGAAIINNYLLQNELYRIGGTALLRGFDEQSIIVSQYHIGTFELRYLLSQNAYAAIF
                     ADGGYAENAAGTTLTADWPYGFGAGINFETKAGI"
BASE COUNT          527 a          298 c          262 g          541 t
ORIGIN      
        1 agatacctgc ttttgtttca aaatttatac ctgcaccaaa accataaggc caatcagcag
       61 ttaaagtggt gcctgctgca ttttcggcat atcccccatc ggcaaaaata gcggcatagg
      121 cattttgtga taataaataa cgcagttcga atgttccgat atgatattga gaaacaataa
      181 tactttgttc gtcaaaacca cgtaataatg cagtaccacc tatccgatat aattcatttt
      241 gcaataaata attattgatg atagcagcgc cccttgtttg caatagtaat gttactcgcg
      301 aaaacagcgg aatataccag ccgagttgat aatgatattt tacagataaa cttttaagat
      361 caattgaatc atataaagtg ctgaaatcaa attccggatt taaaggatct tgtaattcaa
      421 caatggaagc attttcttta attgttttat taccgatagt cgcgccaatt tttaatgcat
      481 atcctttata aggattaaat cgataattca gtttttcaaa attccactct aaaccatagg
      541 ctcgttcaac catatctaaa gatgaaggca attgtaaatt actcaggata tatccggtgt
      601 caattgctaa cacagctgaa ttataaaaat ttgccagcgc tttgaaataa ttattgcctg
      661 tcatttgaaa caacacaccg gcagttgtgg tacgttctaa aaatgtggaa tccttcaaat
      721 acagactaaa tgcgccttcc agaccaatag gcaattttgg taaataggga taaactaatg
      781 aagtttgtaa ttctttagaa gtgctttcca gttttgaaaa atggaaatta aataattcac
      841 ctgcattaaa aacattgtgc aattgtaatc tgccttcgcc ggtaattatt aatcgccctg
      901 ttattgcttc gttgggaata accccaataa ggaaatcgaa agtgctggat tgtttttcat
      961 ccaaatacat aattatttta gcgcgattac ccgaaaattc tattttggta ttttttgatt
     1021 tggtggcaaa cggtatttct tttaacctat tttccaaagt attgagtaat tgctgattaa
     1081 acggagcgcc tggtgtaatt ccggtatatt gaattaaata attggcactt acatttaaat
     1141 tgccaactac cagtaaagta tcaaatacga taaattgatt tttgcgataa attaaatctg
     1201 cactaatcac cgaatcaccc catattatac tatccagttg aattgttgca aatggataac
     1261 caacagattc agaataggtt aacatttttt gcatcatgcc tacaacctcc tgatgattaa
     1321 aaactttacc atttatatct ttcttcctgt atccaacacc tgcgagtgca aattcatcca
     1381 catttgcagt atttaaagta gcccaataca cttttttacc agctactatg gttgcatata
     1441 gggtatcagc attttgtttg gtaaagttcc aggttgcagt taaataccca tcttgctgat
     1501 aagactccag atattgttgt aaatattttt ctacttcaat tttgcttgca aatgcaatag
     1561 gtgcatccaa tccatcatct tttttcgata atggtggatg ttcaactatg agccatgagg
     1621 aatcctgt
//
LOCUS       DAORKW010000714         1625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5221_length_1625_cov_3.650955, whole genome shotgun sequence.
ACCESSION   DAORKW010000714 DAORKW010000000
VERSION     DAORKW010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5221_length_1625_cov_3.650955"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PLB46_17215"
     CDS             complement(<1..1029)
                     /locus_tag="PLB46_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRF78323.1"
                     /translation="MSVQSEIQIRRTFAIISHPDAGKTTLTEKFLLYGGAIQVAGAVK
                     SNKIKKSATSDFMEIERQRGISVATSVMSFEYEGVKVNLLDTPGHKDFAEDTYRTLTA
                     VDSVILVIDCVKGVEEQTMRLMEVCRMRKTPVIIFINKMDRDGKDPFDLLDELEEKLK
                     IRVRPLSWPINMGTHFKGVYSLYHKKLNLFRPGSQRVEDDVLIIEDLKDDLLDKQVGE
                     RDAKQLREDVELIEGVYHEFDRKPYLDADIAPVFFGSAINNFGVQELLETFIQISPVP
                     QGRETDVRFVNADENKFTGFIFKIHANIDPRHRDRIAFLRVCSGHFERNSFYHPVRLD
                     KTLRFINLY"
     gene            1165..1617
                     /locus_tag="PLB46_17220"
     CDS             1165..1617
                     /locus_tag="PLB46_17220"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRF78324.1"
                     /translation="MTKRKDITYVAIQFILLACYFLVFNDRAGGTPLWMQIISGILVV
                     AGTVLVIWPILQLNKFISVFPTPVNGALLVTSGAFKYIRHPIYAGIILGGAGLALMMN
                     NLLQLLIIIMLFVLFEVKSDYEEQQLAKVFKDYEEYKKHTYKFFPGMR"
BASE COUNT          476 a          302 c          328 g          519 t
ORIGIN      
        1 ataaagattt ataaatcgta aagttttatc taaacgcaca ggatgataga agctattgcg
       61 ttcaaaatgc cctgaacaca cacgtaaaaa tgcaatcctg tcgcggtgac gaggatcgat
      121 attcgcgtga attttaaaaa taaacccggt aaatttattt tcgtccgcat taacaaaacg
      181 aacatcagtt tctctacctt gaggcacagg agaaatctga ataaatgtct ccaataattc
      241 ctgcacacca aaattattta tcgcagaacc aaaaaatacc ggtgcaatat ctgcatctaa
      301 atacggcttg cggtcaaatt cgtgatacac accttcaatc agctccacat cttcacgtaa
      361 ttgttttgca tcacgttcac caacttgttt atctaataaa tcatctttta aatcctcaat
      421 aattaataca tcatcttcaa cacgctgcga tcccggacgg aataaattga gttttttatg
      481 atataaggaa tatacacctt taaaatgtgt gcccatgttt atcggccaac ttaaaggtcg
      541 aacacgaatt ttaagttttt cttctaattc atccaataaa tcaaatggat cctttccatc
      601 gcggtccatt ttgttgatga aaataattac cggcgttttg cgcatgcgac acacttccat
      661 taatcgcatg gtttgttctt ccacaccttt cacgcagtcg attactagga taacactatc
      721 cacagctgtt aaggtgcgat aggtatcttc agcaaagtcc ttgtgaccgg gagtatccaa
      781 taaattcact ttaacaccct catattcgaa cgacataacc gatgtggcga cagagatacc
      841 acgctgacgt tcaatttcca tgaagtcgct ggtggccgat tttttaattt tattgctttt
      901 aacagctccg gcaacctgaa tggcgcctcc gtacaagagg aacttttcgg taagggtggt
      961 tttaccggca tcggggtgac tgataatggc aaatgttctc cgtatttgta tctctgattg
     1021 tacgctcatc tcttggtaat tcgggcgcaa agatagtgat ttcaggcttg aaaaggggtg
     1081 ccgggaagac gggaagtcgg gaagttgcgg gggaaatgtt gaccgaactc aaaacccctc
     1141 aacccaattt cctacatttg taacatgaca aaacgaaagg acattactta tgttgctata
     1201 caatttattt tattggcctg ttatttttta gtatttaatg atagggcagg aggtactccg
     1261 ttgtggatgc aaattattag tggaattttg gttgttgcag gaacggtttt ggtgatttgg
     1321 ccaattttac aattaaataa atttattagt gtttttccta caccggttaa tggtgcttta
     1381 ttggtgacga gtggagcgtt taaatatatt cgtcacccga tatatgcggg tattatttta
     1441 ggtggtgcag ggttggcgtt gatgatgaat aatttattgc agttgctgat aattattatg
     1501 ttgtttgttt tatttgaagt gaaatcggat tatgaagagc aacagcttgc taaagtgttt
     1561 aaggattatg aggaatataa aaaacatact tacaaattct ttcctgggat gaggtagtgg
     1621 gtttg
//
LOCUS       DAORKW010000715         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5278_length_1616_cov_4.997438, whole genome shotgun sequence.
ACCESSION   DAORKW010000715 DAORKW010000000
VERSION     DAORKW010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5278_length_1616_cov_4.997438"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..574
                     /locus_tag="PLB46_17225"
     CDS             26..574
                     /locus_tag="PLB46_17225"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRF78325.1"
                     /translation="MKIYTKKGDGGETSLIGGTRVPKHHIRVDAYGSTDELNVYIGLI
                     ADQQIDTGYKNVLNEIQDRLFTIGAALASDPNKSKMAIPDLKESDITFLEEQIDTMEL
                     KLPVLQNFILPGGHTTVSFCHLARVICRKTERIVVHLAENEQVEPMIITYLNRLSDYL
                     FVLSRKIARDLDVEEITWRPRG"
     gene            730..951
                     /locus_tag="PLB46_17230"
     CDS             730..951
                     /locus_tag="PLB46_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HRF78326.1"
                     /translation="MYWTLELASYLEDAPWPATKDELIDYAIRSGAPIEVIENLQELE
                     DEGEFYEGIEDIWSDYPTHDDFYFNEDEY"
     gene            complement(1029..>1616)
                     /locus_tag="PLB46_17235"
     CDS             complement(1029..>1616)
                     /locus_tag="PLB46_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRF78327.1"
                     /translation="LPKNIKRKPDEEDKERYQTVFAKHEGAVAAPTAGMHFSRELMKR
                     LEIKGINFAELTLHVGLGTFRSIDVEDLSKHKMDAEYFKVDQKCVEVVNKGLDENRRI
                     CAIGTTSMRACESAVAATGRLKTEEGWTNLFIHPPYDFTICNSMVTNFHLPKTSLVIM
                     VAAFMGYDLTMEAYKLAMKEKYRFFSYGDAMLIVD"
BASE COUNT          493 a          302 c          323 g          498 t
ORIGIN      
        1 aaaacaaatc tattaccttt gcgctatgaa aatttatacc aaaaagggtg acggtggtga
       61 aacttcatta ataggaggaa ctagggttcc taagcaccat attcgcgtag atgcttatgg
      121 ttcgacggat gagctcaatg tatatattgg attaattgcc gaccagcaaa ttgatacagg
      181 gtataaaaat gtgttaaatg agatacagga caggctgttt actattggcg cagcacttgc
      241 atctgacccc aataagtcga aaatggccat tccagacctc aaggaaagcg atatcacctt
      301 tttggaggaa cagattgata ccatggagct caaattgcct gttttacaga attttatact
      361 tcccggaggg catactacgg tgtcgttttg ccatttagcg agagttatct gcagaaaaac
      421 tgaacgtata gtggtgcatt tggctgaaaa tgagcaagtt gagccaatga taattactta
      481 cctcaataga ttgagcgatt acctctttgt tttatcgcgc aaaatagctc gcgatttgga
      541 tgttgaagag ataacctggc gcccgagagg ttaattttcc acagcaaaac ctataatttt
      601 tcttaatatg cggcatattt aggggctgat tgtaaattat ttttgatata agcacaattt
      661 gttcatattg gtacttgcaa aagattaaaa aaaatctatt tttgcgccct aattaaaaaa
      721 caggttaata tgtattggac acttgaactc gcctcatatc ttgaagatgc gccatggccg
      781 gcaacaaaag atgaattgat tgattatgcc attcgttcgg gagctcctat tgaagttatt
      841 gagaacttac aggaacttga ggatgagggt gaattttatg aagggattga agacatctgg
      901 agtgactacc cgacacatga cgacttttac tttaatgagg acgaatattg aaacaaaccg
      961 ttaaacgtat tccaaaaaaa aatcctgcaa ttgcaggatt tttttttggt gtagtctttt
     1021 catacaaatt aatctactat cagcatagca tctccgtaag agaagaagcg atatttttct
     1081 ttcattgcca atttataggc ttccatagtt aaatcgtaac ccataaaagc agccaccata
     1141 ataaccaagc tggttttagg aaggtggaaa ttggtaacca tagaattaca aatagtgaaa
     1201 tcgtaaggag gatgaataaa taaatttgtc cagccttctt cagtttttaa tcttccggta
     1261 gctgcaacag cgctttcgca tgcacgcata ctggtagttc cgatagcaca aattcggcgg
     1321 ttttcatcta aacctttgtt tactacttca acacattttt ggtctacttt aaagtattcg
     1381 gcatccattt tatgtttcga taaatcttcc acatcaatac ttctaaatgt gcctaaacca
     1441 acgtggagtg tcaattcagc gaaattaatc cccttaattt cgaggcgttt cattaactcg
     1501 cggctaaagt gcatacccgc agttggcgct gccacggctc cttcatgttt agcgaaaacg
     1561 gtttggtaac gctctttatc ctcttcatcc ggtttgcgtt tgatattctt tggaag
//
LOCUS       DAORKW010000716         1613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5304_length_1613_cov_4.148267, whole genome shotgun sequence.
ACCESSION   DAORKW010000716 DAORKW010000000
VERSION     DAORKW010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5304_length_1613_cov_4.148267"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLB46_17240"
     CDS             <1..558
                     /locus_tag="PLB46_17240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78328.1"
                     /translation="VAAAAGQHFVMILTNEGAVFTCGNNSDGQLGDGTVENKSLAIGV
                     DGLSGILEIAAGGKTAVVLDNDGNMFAWGDNIVGQIGDNTLSDKWTPTLVTSACSMLL
                     ATNDLTESQSGFDVYPNPADDYLFIKHTDVNNHELQLIDINGKIISSIISNNSTNKID
                     VSYLSAGIYLLRDPNYANAITIIVE"
     gene            complement(629..>1613)
                     /locus_tag="PLB46_17245"
     CDS             complement(629..>1613)
                     /locus_tag="PLB46_17245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78329.1"
                     /translation="SIDMDADSTSDFLLFANSNTAGNWSWVYAIGNLGSGYGGPSNMI
                     AGYSGAILPYGSALDAGDLIGPGLSFVSNYNNVGWLASMYSSVTYAPFANTGDKYLGV
                     KFLSGGTLHYGWMRLSCTVAPVTYTIRDYAYEATANTPIIAGDGIPCATPPDIVSATS
                     LGPTGEKLKWTAESGIDYYQVYYRPVGAPNWLKKKSTSNQKNITGLTCATNYQWKVRS
                     NCAGTFSAFSATSTFTTAACRMDDMADVTPEITLYPNPAADFMYIDITTEITSDLEVF
                     VTDMSGRTISGLSFTNDEDMLVLDVAALPAGLYMLSVRQGDVTTTANFVKQ"
BASE COUNT          527 a          293 c          320 g          473 t
ORIGIN      
        1 gttgctgctg cggccggtca acattttgtt atgatactta ctaatgaagg agctgtattt
       61 acttgtggaa ataatagtga tgggcaatta ggtgatggca cagttgaaaa taaatcgttg
      121 gcgattggtg tagacggatt aagtggtatt ttagaaatag cagcgggtgg aaaaacagca
      181 gttgtgttgg ataacgacgg taatatgttt gcttggggcg ataatattgt agggcaaatt
      241 ggtgataata cgttatcaga taaatggacg ccaacacttg ttacgtctgc atgttcaatg
      301 ttattagcta caaatgattt aactgaatca cagtctggat ttgatgtgta tcctaatccg
      361 gcggatgatt atctatttat taaacataca gatgtaaaca atcatgaact gcaattgata
      421 gatataaatg ggaaaattat ctcatccatt atttcaaaca attcaacaaa caaaatcgac
      481 gtatcttatt tatctgctgg tatttattta ttacgtgatc ctaattacgc aaatgctatt
      541 acaattattg ttgaataatt taattactat ttacaaaaaa agaggctgcc ctaaaggaca
      601 gcctctgaat atgagtagtt aaacttaatt attgtttaac aaaattcgca gtagtagtta
      661 catcaccctg acgaacagat aacatataca aacctgcagg tagagccgct acatccaata
      721 ctaacatatc ttcatcattt gtgaagctaa gtccgctgat tgtgcgacca gacatatcgg
      781 ttacaaaaac ttccagatca ctggtaattt cagttgttat atcgatatac atgaaatcgg
      841 cagcagggtt tggatataaa gtgatttccg gtgtaacatc agccatatca tccatacggc
      901 aagccgcagt ggtgaatgtt gaagtagcag agaaagcact aaatgttccg gcgcaattgc
      961 ttctaacttt ccattggtaa ttagtagcac aagttaaacc ggtaatattt ttttggttag
     1021 aagttgattt tttcttcaac caattaggag caccaacagg acgataataa acctggtagt
     1081 aatcaattcc tgattcagca gtccatttca gtttttcacc ggttggtcct aaagaagttg
     1141 cagaaacgat atcaggtggt gtagcacaag gaataccatc accggcaata attggtgtgt
     1201 tggcagtagc ctcgtaagca taatctctga tagtgtaagt tacaggagca actgtacagc
     1261 ttaagcgcat ccaaccatag tgtaaagttc caccagaaag gaatttaaca ccaaggtatt
     1321 tatcgccggt attggcaaat ggtgcgtaag ttacactgct atacattgat gctaaccaac
     1381 ctacgttgtt gtaattagat acaaagctca agcctggtcc aattaaatcg cctgcatcta
     1441 atgcactacc gtaaggtaaa atagcaccag aataaccagc aatcatgttt gatggtccac
     1501 cataacctga acctaaattt ccaattgcat acacccaact ccagttacct gcagtattgg
     1561 aattcgcaaa aagaagaaaa tcactggtag aatcggcatc catgtcaatt gac
//
LOCUS       DAORKW010000717         1600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5370_length_1600_cov_4.840777, whole genome shotgun sequence.
ACCESSION   DAORKW010000717 DAORKW010000000
VERSION     DAORKW010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5370_length_1600_cov_4.840777"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLB46_17250"
     CDS             <1..463
                     /locus_tag="PLB46_17250"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HRF78330.1"
                     /translation="VDFEMRKTKFKLLLGGQLINYNQEVYENEPDRPMVQTITPFAEL
                     TYKFDRKKSMRVELQYQQCEQDYGSWVYGLLEYNIAPKWSFAVSDMWNYEPLRTEEAL
                     HYYSIFTGYTHKSSRFTLNYVKQIAGIVCTGGVCRYEPAFSGLKAGLVTTF"
     gene            481..1347
                     /locus_tag="PLB46_17255"
     CDS             481..1347
                     /locus_tag="PLB46_17255"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HRF78331.1"
                     /translation="MKNITNKFLTIVSIAAIVVITFNGCKEVGPEINLTETDYITYLE
                     TPEAASQKIILLEDFTGAACPNCPDGHIAIADVIDANPGKVVAIAEYNYFGDPLYGDQ
                     IFVTDEAYSLDDYLGPVTGWPAIFIDRKDFGGDDYLAELPENVAPFSDEQLSLSSPVN
                     LYVDNTYDAATRLLTTHVTIKYTDDVTTTNNLSIALTESGIIAAQIDDNVGGEVPDYE
                     HNHMLRKMLTYYAGDALPEENIAGRVYEFEYSYTLNADWNADNMHVVAFVHNAETDNK
                     EVLQVAEVEVVE"
BASE COUNT          547 a          238 c          309 g          506 t
ORIGIN      
        1 tgttgatttc gaaatgcgca aaacgaaatt taaattatta ttaggcggac aattaattaa
       61 ttacaatcag gaagtttatg aaaacgaacc tgatcgccca atggttcaaa ctataacacc
      121 atttgctgaa ttaacttaca aattcgacag aaaaaaatcg atgcgtgttg aattacaata
      181 tcagcaatgc gagcaggatt atggcagctg ggtgtatgga ttattagaat ataatattgc
      241 accaaaatgg agttttgcag taagtgatat gtggaattat gaacctttac gcacagaaga
      301 agctttacat tattattcga tatttacggg atacacgcat aaatcgtcac gatttacgtt
      361 aaactatgta aaacaaattg ccggtatagt ttgtacaggt ggtgtttgcc gttacgaacc
      421 agcattcagc ggattaaaag ccggattagt aacaacattt taattgatta aaatatgttt
      481 atgaaaaata taacaaataa atttttaaca atagtttcca ttgctgctat tgtagtgata
      541 acgtttaatg gatgtaaaga agttggaccg gaaattaacc tcactgagac tgattatata
      601 acatatttag aaacaccgga agctgcctca caaaaaataa ttttgttaga agattttacc
      661 ggcgcagctt gtccgaattg ccctgatggc catattgcca tagcagatgt aattgatgct
      721 aatccaggta aagttgtggc aattgcagaa tataattatt ttggtgatcc attatatggt
      781 gatcaaatat ttgtaacaga tgaagcatat agtctcgacg attatttagg acctgtaacc
      841 ggatggcctg ccatttttat tgacagaaaa gattttggtg gtgatgacta tcttgctgaa
      901 ttaccagaaa atgtagcacc tttttctgat gaacaattaa gcttaagcag cccggttaat
      961 ttgtatgtag acaatacgta tgatgctgct accagattat taactacaca tgttactatt
     1021 aaatataccg atgacgtaac aacaacaaat aatttaagta ttgcattaac cgaaagtggc
     1081 attattgcag cacaaatcga cgataatgtt gggggtgagg tacctgatta cgaacacaat
     1141 cacatgctaa ggaaaatgtt aacctattac gccggggatg cattaccgga agaaaatatt
     1201 gccggcagag tatatgagtt tgaatattct tatactttaa atgctgattg gaatgctgac
     1261 aacatgcatg tagttgcatt tgtgcataat gcagaaactg ataataaaga agtattacag
     1321 gttgcggaag ttgaagttgt ggaataattg taaattatat ttttatataa atcactgtaa
     1381 caaaaaaagg gcttcgtttg aagccctttt ttatttattt aattgtcggc attaaatttt
     1441 aagttggaat tgtgcaacaa acatatttgc tgaagttatt cctccgggag aaatagcact
     1501 agcccaattt gtattaatta cgtttaattg taatcttgcg gtattctcag gtaaaaatgt
     1561 aaacccgata ttacctgtta tagtagtata gtcgttatcg
//
LOCUS       DAORKW010000718         1588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5455_length_1588_cov_4.405088, whole genome shotgun sequence.
ACCESSION   DAORKW010000718 DAORKW010000000
VERSION     DAORKW010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5455_length_1588_cov_4.405088"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="PLB46_17260"
     CDS             <1..1516
                     /locus_tag="PLB46_17260"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRF78332.1"
                     /translation="TDGVVIHYEHVGLNDAGYPYHLGRTATHEIGHWLNLRHIWGDDS
                     NCAGTDFVDDTPNQKVETYGCPGFPKTDACSATAPGIMFQNYMDYSDDACMNIFTEGQ
                     KERMMALFEPGGARYGLLSSDGCGLQPYDAQAISSLPGGTICDLSVTPKVVIKNHGAV
                     ELTSMDINYSVDGGTVSTYEWIGSLLSAETEEVTLPAVAVAEGDHTLEVTLDNPNGFI
                     DADFSDNTTSTDFTVNLGTLPLPVVEGFEATGFPYAGYSLSNPDGGETWERTTTAAAL
                     GSASIFMNHFNYDSPGEIDEFVLPAYDLTGLADIVFTFDVAYALYTADGSMSDTLEVL
                     VSDDCGDNWTSVYKKANPDLQTAPNHTISFKPEDDEWRNESINLDAYIGTEQFFVKFR
                     TITDYENNLYIDNININNGQIQAVNDNQPDFAMNLYPNPVNDNVQIRYNVPVNGNGSL
                     SITDVLGKVVYSTEIGVSYGVNVVNVNTAAFASGYYQVTLNCEGLVASESLVKE"
BASE COUNT          467 a          292 c          344 g          485 t
ORIGIN      
        1 aactgatggt gttgtaatac attacgaaca tgtaggtttg aacgatgcag gatatcctta
       61 ccacttgggt cgcaccgcta ctcacgaaat tggccattgg ttaaacttgc gccatatttg
      121 gggtgatgac agcaattgcg caggtacaga tttcgtggat gatacgccaa atcaaaaagt
      181 tgaaacttat ggttgtcccg gatttccaaa aacggatgct tgctctgcaa cagcaccggg
      241 aatcatgttc caaaactata tggattatag cgatgatgct tgtatgaata tttttactga
      301 aggccaaaaa gagcgtatga tggcattgtt tgaaccaggt ggagcccgat acggactgtt
      361 aagcagcgat ggatgtggtt tacaacctta tgatgcacaa gcaatttcat cactcccagg
      421 aggtactatt tgcgatttaa gtgtaactcc aaaggttgta atcaaaaacc atggtgctgt
      481 tgaattaact tcaatggata ttaattactc tgttgatggt ggtactgtaa gcacttacga
      541 atggataggc tctttgttat cagctgaaac tgaagaggtt actttgcctg cggtagcggt
      601 agcagaaggc gatcacacgt tagaagtaac actcgataac ccaaatgggt ttattgatgc
      661 tgatttttca gataatacaa cttcaaccga ctttaccgta aacttaggca ccctgccttt
      721 accggtagta gaaggttttg aggctacagg attcccttat gcaggatatt cactttcaaa
      781 ccctgatggt ggtgaaactt gggaaagaac tacaaccgcc gctgcactag gttctgcatc
      841 tatttttatg aaccacttta attacgacag tccgggagaa attgatgaat ttgtgttacc
      901 tgcttacgac ctaaccggtt tagcagatat tgttttcaca tttgatgtgg cttatgcgct
      961 ttatactgct gatggctcca tgtccgatac gcttgaagtt ttggtaagtg atgactgtgg
     1021 tgataactgg actagtgttt acaaaaaagc taatcctgac ttacaaaccg cacctaatca
     1081 taccatcagc tttaaacctg aagatgatga atggagaaat gaatcaatca atttagatgc
     1141 ctatatcgga actgagcagt ttttcgtaaa attccgcact attactgatt atgagaacaa
     1201 tctttatatc gataatatca atatcaataa tggtcaaatt caagcagtta atgataacca
     1261 gcctgatttt gcgatgaacc tctatcctaa cccggttaat gataatgttc aaattcgata
     1321 taatgtgccg gttaatggca atggcagttt atctatcacc gatgtgttgg gtaaagtagt
     1381 ttactccact gagattggcg tatcgtatgg cgttaatgta gtgaatgtaa acacagcagc
     1441 atttgcttca ggttattatc aagtaacgct gaattgtgaa ggtttagttg caagtgaaag
     1501 tctggtgaaa gaataatatt cctcacctaa tcatatttgg agccggtttg ttatacagac
     1561 cggcttttta attgctaaat cgctttag
//
LOCUS       DAORKW010000719         1588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5456_length_1588_cov_3.581213, whole genome shotgun sequence.
ACCESSION   DAORKW010000719 DAORKW010000000
VERSION     DAORKW010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5456_length_1588_cov_3.581213"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLB46_17265"
     CDS             complement(<1..619)
                     /locus_tag="PLB46_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78333.1"
                     /translation="MEDNKKYLNQTMDEIVFDNRNKSYGAFFLRHLTKRNTVKALIFI
                     FCLSSVLTGFSYVGLGFLKKKTDEVAIERVVTLTEPPPLDEEAPPPPPPPPPPPPVRP
                     TVKFVEMVVKKDEEVVEDEVIAVDEIKADISTVNQEGDEDADIVIEEVKVVQQPVVEE
                     KKVYDFLEQMPEYPGGVKNLYKYLGDNIKYPRMARDNGIEGTVYIK"
     gene            complement(690..1178)
                     /locus_tag="PLB46_17270"
     CDS             complement(690..1178)
                     /locus_tag="PLB46_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRF78334.1"
                     /translation="MSTRVDFTPMVDLAFLLISFFMLTTTLNKPVAMELNMPKKDEQV
                     KTDVKESQVINLLLDKDDKIWYYEGQVVYDMKTTNYAADGVREVLYDKQKDVGRKHGN
                     PEKMIVIIKMTEGASYKNMVDILDEMDITNTKIYAIQDIADLELEAIANGGKLDIVPT
                     PQ"
     gene            complement(1262..>1588)
                     /locus_tag="PLB46_17275"
     CDS             complement(1262..>1588)
                     /locus_tag="PLB46_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78335.1"
                     /translation="GIRGVPAGDWGSVKDFSGVPIDSANNQLRDWIQSARWADRLDKQ
                     ESGATDREDIRVAIKGDKTTNVKAVQEVIKILTEEPVNIHTFNLITSLEGPVAAETAG
                     TEPPAK"
BASE COUNT          406 a          362 c          297 g          523 t
ORIGIN      
        1 atttgatata cacggtgcct tcaataccgt tatcacgcgc catacgcgga tatttgatgt
       61 tatcacctaa atatttataa aggtttttaa caccacccgg atattccggc atttgttcaa
      121 ggaaatcgta tacttttttc tcttcaacaa ccggttgttg tactactttt acttcttcaa
      181 taacgatatc agcatcttca tcaccttcct gattaacggt agagatgtcg gctttgattt
      241 cgtcaactgc aattacctca tcttcaacta cttcttcatc ttttttcaca accatctcca
      301 cgaatttcac ggttgggcga accggtggtg gtggtggtgg cggaggtgga ggaggaggtg
      361 cttcttcatc cagcggaggt ggttcggtta aggtaaccac gcgctcaata gccacttcgt
      421 cagttttttt cttcaaaaac cccaaaccca cataactaaa gcctgtaagc actgagctca
      481 ggcagaaaat gaagatgagt gcctttacag tgtttctttt agtcagatga cgtaaaaaga
      541 acgcaccata agacttgttg cgattgtcga aaacaatctc atccatggtt tggtttaaat
      601 actttttatt atcttccata atctcggata attttttatt tggatgcctt cggaaccgaa
      661 attccataaa acatctaaaa attatttatt tattgtggtg ttggcacgat atccaacttt
      721 ccaccattgg caattgcttc cagttctaaa tcagcgatat cctgaatggc atagatttta
      781 gtattcgtaa tatccatttc atcgagaatg tccaccatat ttttataaga agcaccttcg
      841 gtcattttaa tgataacaat cattttttcc gggttaccat gttttctgcc tacgtctttt
      901 tgtttatcgt ataaaacctc tcttacacca tcagcagcat aattggtggt tttcatatca
      961 taaacaacct gaccttcgta ataccagatt ttatcatcct tatctaacaa taagttgatt
     1021 acctgagatt ccttaacgtc ggttttcacc tgttcgtctt ttttaggcat attcagctcc
     1081 atggccaccg gcttgttcag cgtggtagtc aacataaaga aggaaatcaa caggaacgcc
     1141 aaatccacca ttggggtaaa atcgaccctg gttgacattt ttttggaacg aaccttgcct
     1201 ttcccgtgct tactgtgact ccctttctcg ggcgtttgta tatcagccat agctagttct
     1261 tttatttagc aggaggttca gtgcctgctg tttcagcagc aaccggccct tcgagcgatg
     1321 tgattaaatt aaatgtatga atgttgacag gttcttcagt cagaattttg attacttcct
     1381 gcactgcttt aacattagtg gttttatcgc ctttaatggc aaccctgatg tcttcgcggt
     1441 cggtagcccc tgattcttgt ttgtctaaac ggtcggccca gcgtgcggat tgtatccagt
     1501 cgcgtaattg gttattggcc gaatcgatag gcacaccgga aaaatctttc accgaccccc
     1561 aatcgccagc aggaacaccc cgtattcc
//
LOCUS       DAORKW010000720         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5469_length_1586_cov_4.363161, whole genome shotgun sequence.
ACCESSION   DAORKW010000720 DAORKW010000000
VERSION     DAORKW010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5469_length_1586_cov_4.363161"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..166
                     /locus_tag="PLB46_17280"
     CDS             11..166
                     /locus_tag="PLB46_17280"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HRF78336.1"
                     /translation="MDYETDQGFGMVRTEILCSKCGGHLGHVFDDGPTETGNRFCVNS
                     ASLKFKK"
     gene            complement(171..1217)
                     /locus_tag="PLB46_17285"
     CDS             complement(171..1217)
                     /locus_tag="PLB46_17285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF78337.1"
                     /translation="MKNASPSRIVLASSGIILLILLVLELAWYLFPVLQFKAYQLVII
                     PLIAFFCIFLIFYLAVEKFIYRKIKLIYKNIHALKSTKEIADEKMKMSSDVISEVNNE
                     VIEWAKERSSEIDLLIKQADYRKEFLGNVSHELKTPIMSIQGYLETLSDGGIEDPTIN
                     SMYIDKALKNVDRMIMIIDDLVNISMLESGELALNYKKFDINALVKEVYESLEMQASA
                     AKTKLAIKEGCDHVFNVYADEKLIREVLVNLISNAIKYGNKEGFVHAGFYNMGDNILI
                     EIADNGLGIAKEHLNRVFERFFRIEKSRSRDMGGTGLGLSIVKHIIEAHNQNVNVRST
                     IGEGSTFGFTLQKA"
     gene            complement(1225..>1586)
                     /locus_tag="PLB46_17290"
     CDS             complement(1225..>1586)
                     /locus_tag="PLB46_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF78338.1"
                     /translation="PIKPRIRLSRIKALLRRTAVMEPVNEAVTYGDLSIDEEKFLVYV
                     KGDPISLAKKEFELIQLLASKPGKVFTRQEIFNRIWGMDVIVGDRTIDVHIRKIREKI
                     GEDFIKTIKGIGYKFDH"
BASE COUNT          484 a          331 c          238 g          533 t
ORIGIN      
        1 aaaaggaaca atcgattacg aaaccgacca gggttttgga atggtgagga cagaaatttt
       61 atgcagtaaa tgtggaggtc atttaggaca cgtgtttgat gacggtccaa cagaaacagg
      121 caacagattt tgtgtaaatt ctgcttcgtt aaagtttaag aagtaataga ttaggccttt
      181 tgcagggtaa aaccaaaagt ggagccctct cctattgtgc tccttacatt tacattttgg
      241 ttatgtgctt caattatatg tttcacaata gataaaccta atccggttcc ccccatatcg
      301 cgggatctac ttttttctat gcggaaaaaa cgttcaaaaa cacgatttaa gtgttctttt
      361 gcgataccta aaccattgtc tgcaatttca atcaggatat tatcacccat attgtaaaat
      421 ccggcgtgca caaaaccttc tttattaccg tacttaatcg catttgaaat taaattaacc
      481 aacacttcac gaattaattt ttcatcagca taaacattaa aaacatggtc gcaaccttct
      541 ttaatagcca gtttagtttt agcggccgat gcctgcattt ccagcgactc gtaaacctct
      601 ttgactaagg cattgatatc aaatttttta taatttaatg ccaactcgcc gctttccaac
      661 atcgaaatat tcaccaagtc atcgataatc ataatcatac gatcaacatt ttttaatgct
      721 ttatcgatgt acatgctgtt gatagttggg tcctctattc cgccgtcaga aagcgtttcg
      781 aggtatccct gaatgctcat gatcggcgtt tttaattcat gacttacgtt gcctaaaaat
      841 tcttttctgt agtccgcctg ttttatcagc aaatctattt cactacttct ttccttcgcc
      901 cattcgataa cttcattatt cacttcagaa ataacatccg acgacatttt cattttttca
      961 tcggcaattt ctttggtcga ttttagtgcg tgaatatttt tataaatcaa tttaattttg
     1021 cggtaaataa atttttcaac cgctaagtag aagattaaaa aaatgcaaaa aaatgcaatt
     1081 agcggtataa ttaccagttg ataagcctta aactgtaata caggaaataa ataccacgcc
     1141 aattccagca ccaacagaat taataaaata attccagatg aagccaacac tattcggctc
     1201 ggtgaagcat ttttcatcaa ctaattaatg gtcaaattta taaccaattc cttttattgt
     1261 tttaataaaa tcttcaccaa ttttttctct tattttacga atatggacat ctattgtcct
     1321 gtcccctaca attacatcca ttccccaaat acggttaaaa atctcctgtc tggtaaacac
     1381 tttacccggt ttagaagcta ataattgtat gagttcaaac tctttttttg ccagtgaaat
     1441 cgggtcacct ttaacatata ccaggaattt ttcttcgtca atgctcaaat cgccatatgt
     1501 tacagcttcg ttaaccggct ccattacagc tgttctccgc aacaatgcct ttatccttga
     1561 caaacgtatt ctcggcttta taggct
//
LOCUS       DAORKW010000721         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5533_length_1577_cov_5.643890, whole genome shotgun sequence.
ACCESSION   DAORKW010000721 DAORKW010000000
VERSION     DAORKW010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5533_length_1577_cov_5.643890"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLB46_17295"
     CDS             <1..186
                     /locus_tag="PLB46_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78339.1"
                     /translation="KPHPNLEHRRHHRPRCHFLGNRHYRLKPNQTYIKIRLPADFFLD
                     LILSCIGCRKQSISQIA"
     gene            complement(258..848)
                     /locus_tag="PLB46_17300"
     CDS             complement(258..848)
                     /locus_tag="PLB46_17300"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRF78340.1"
                     /translation="MFTGIIETVGEVVNLHREAGNLFITMNSAIGNELTVDQSVAHNG
                     VCLTVVKTHQTQHVVTAIEETLSKTNLGNLHVGDLVNLERCLQIGGRLDGHIVQGHVD
                     GKAKLLSVLEQNGSYVLTFEIDVVFRNLIVEKGSICLNGISLTLINVTDNTFAVAIIP
                     YTWEHTNIQTLKIGDEVNVEFDIIGKYVNRMQQGNE"
     gene            complement(872..>1577)
                     /locus_tag="PLB46_17305"
     CDS             complement(872..>1577)
                     /locus_tag="PLB46_17305"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF78341.1"
                     /translation="VATLPDTLINLSQIQCGVPVDNEPPCAPVLTVNNICANDENVVV
                     DDDDLKNELSWTNPNNSCADDVVKYIIYYASQEGQQLGVLDQVIDPNDTSFTHEALLS
                     LAGCYAIVAVDSFGNESALSNVVCVDNCSDYNLPNAFTPNGDGFNDVYTPRLPIRFID
                     HVDMKIFDRWGSLVFTTTDPMINWDGKDSATGIDVAEGVYFYKCDVFEITVNGVTAFP
                     EPKDGYIHLVRQDKSE"
BASE COUNT          498 a          344 c          240 g          495 t
ORIGIN      
        1 aaaccgcacc ctaacctgga gcatcgtcgg catcatcgcc ctcgctgcca ttttttgggc
       61 aaccggcatt atcggctaaa accaaaccaa acctatatca aaatccgcct tccggcggat
      121 ttttttttgg acttaatttt atcttgtatt gggtgtcgta aacaatcaat tagtcagatt
      181 gcataaaagc atttcccctt taaatttgtt gctttgatta cgtttattgt tattattaaa
      241 aataacaacc acactgttta ttcattgccc tgctgcatgc gattgacata tttaccaata
      301 atatcaaact ctacattcac ctcatctcct attttaagtg tttgaatatt agtatgttcc
      361 cacgtgtatg gaattatagc aacagcaaaa gtattgtctg taacatttat caaagtgaga
      421 ctaattccat ttaagcaaat acttcctttt tcaacaatta aattacggaa tactacatct
      481 atttcgaagg ttaatacata actaccattt tgctccagca ccgataataa tttagcttta
      541 ccatccacgt gtccctgcac aatatgacca tctaaacgac cgcctatttg cagacatcgt
      601 tcaaggttta ccaaatcgcc aacatgtaaa tttcctaaat tagttttaga taatgtttct
      661 tcaattgccg taacaacatg ttgggtttga tgtgttttta ctaccgttaa acaaacacca
      721 ttatgtgcta cactctggtc aacagttaat tcgttaccaa ttgcagaatt catggtaatg
      781 aataaattac cagcttcgcg gtgaaggttc accacttcgc caactgtttc tattattcct
      841 gtaaacatga ggtaaagtta agcagaagaa attattcaga tttatcctga cgaactaaat
      901 gaatataacc gtctttgggt tcgggaaatg cagtaacacc gtttaccgta atttcaaata
      961 catcgcattt gtaaaaatac actccctcag ctacatcaat acccgtagcg ctatccttgc
     1021 catcccaatt aatcataggg tcggtagtag taaatactaa acttccccaa cggtcgaata
     1081 ttttcatatc tacgtgatct ataaaacgaa taggtaaccg aggtgtatac acatcattaa
     1141 atccgtcgcc gtttggtgta aatgcattcg gtaaattata atcagaacaa ttatctacac
     1201 aaacaacatt actaagtgcg ctttcatttc caaatgaatc gactgccaca attgcataac
     1261 atcctgccaa cgatagcaat gcttcatgtg taaacgaagt gtcgttagga tcaatcacct
     1321 gatcaagcac gcctaattgc tgaccttcct gtgatgcata ataaataata tatttcacca
     1381 catcatcggc gcatgaatta ttcggatttg tccacgataa ttcgtttttc aaatcatcat
     1441 catcaacaac aacattttca tcgttagcgc aaatattatt aaccgtaagc accggagcac
     1501 aaggtggttc attatctacc ggaacaccac attgtatttg tgaaagattg ataagtgtat
     1561 caggcaaagt tgcaacg
//
LOCUS       DAORKW010000722         1571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5575_length_1571_cov_4.228232, whole genome shotgun sequence.
ACCESSION   DAORKW010000722 DAORKW010000000
VERSION     DAORKW010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5575_length_1571_cov_4.228232"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLB46_17310"
     CDS             complement(<1..180)
                     /locus_tag="PLB46_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78342.1"
                     /translation="MHLIEVNNKDTAKWFHELPFQLYGDNPNYIAPIRQEIDRIFDPE
                     KNHLYKTGDAVRYIVN"
     gene            546..>1571
                     /gene="atpD"
                     /locus_tag="PLB46_17315"
     CDS             546..>1571
                     /gene="atpD"
                     /locus_tag="PLB46_17315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRF78343.1"
                     /translation="MPGKGKIKQIIGAVVDVSFEEGNLPEILNALEVKRETGDSLILE
                     VQQHLGEDSVRAIAMDSTDGLVRGTEVIDLGANISMPTGNEIRGRLFNVVGKAIDGMK
                     AVNSEKPYPIHRKPPLYEDLSTESEVLFTGIKVIDLIEPYAKGGKIGLFGGAGVGKTV
                     LIMELINNIAKGYAGMSVFAGVGERTREGNDLLREMIESNVIRYGEEFKHSMEAGGWD
                     LSKVDYSQLNQSQATLVFGQMNEPPGARARVALSGLTVAEYFRDGDPNEAKGRDILFF
                     IDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGVMQERITSTKRGSITSVQAVYV
                     PADDLTDP"
BASE COUNT          450 a          304 c          343 g          474 t
ORIGIN      
        1 gttaacgata taacgaacgg catcaccggt tttataaaga tggttttttt caggatcaaa
       61 tatgcggtct atttcctgac gaatgggagc tatataattt ggattgtcgc cataaagttg
      121 aaatggtaat tcgtgaaacc atttggcggt gtctttgttg tttacttcta ttaaatgcat
      181 ttggggtaaa actaatatat ctgcaacaaa atgtcgaaat ggaatgccta accgagagaa
      241 aaaatagggt aaatctgcct aattccccag ttgatcaatc tgttttatgc tgcctaactt
      301 gttgatttta tacctcttta tctcatatat ttattattaa attaagccgc tataacaagg
      361 tttagccctg ttgaaggctt tttttattaa aagaagaaca gggatttgtg gttggatagc
      421 tgcctaacca tcaattttta ctcgcttgta tctacaaatc tttgtgtata atacctttga
      481 attccaaatt tccccccgta tctttgccgc cgaattgaaa aacgtccttt taaattatat
      541 atataatgcc aggaaaaggt aaaatcaaac aaatcatcgg cgcagttgtc gatgtttctt
      601 tcgaagaagg taatcttccg gaaatcctca acgcccttga ggttaaacgc gaaacaggcg
      661 attcgttaat tcttgaagtg caacagcacc ttggagaaga cagcgtgcgt gcaatcgcaa
      721 tggactctac cgatggttta gttcgcggaa ctgaagtaat tgacttaggt gccaatattt
      781 cgatgcctac aggtaacgaa atccgtgggc gccttttcaa tgtagtaggt aaagcaattg
      841 acggtatgaa agccgtaaat tctgaaaaac cttatcctat ccaccgcaaa ccgccgctgt
      901 atgaagattt atctacagaa tcagaggtgt tgtttaccgg tatcaaagta atcgacttga
      961 ttgaacctta cgcaaaaggt ggtaaaatcg gtctctttgg tggtgccggt gtgggtaaaa
     1021 cagtattaat tatggagctc atcaacaata tcgccaaagg ttatgcaggt atgtcggtat
     1081 ttgccggcgt aggtgaaaga acccgcgaag gaaacgattt gttgcgtgag atgattgagt
     1141 ctaacgttat ccgttacggt gaagaattta aacattctat ggaagccggt ggatgggact
     1201 taagtaaagt tgactacagc caattaaatc aaagtcaggc tactttagtg ttcggtcaaa
     1261 tgaacgaacc tcctggagcg cgcgcccgtg ttgccttatc aggtttaacc gttgctgaat
     1321 acttccgcga tggcgaccct aatgaagcta aaggtcgtga tatcctgttc tttatcgata
     1381 atatcttccg tttcactcag gcaggttctg aggtatcagc gcttttaggt cgtatgcctt
     1441 cagcggtagg ttaccaacca acacttgcta ccgaaatggg tgtgatgcaa gaacgtatta
     1501 cctcaactaa acgtggttca attacttcag tgcaggccgt ttacgttcct gcggatgact
     1561 taactgaccc t
//
LOCUS       DAORKW010000723         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5613_length_1566_cov_5.183984, whole genome shotgun sequence.
ACCESSION   DAORKW010000723 DAORKW010000000
VERSION     DAORKW010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5613_length_1566_cov_5.183984"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /gene="glpK"
                     /locus_tag="PLB46_17320"
     CDS             <1..1344
                     /gene="glpK"
                     /locus_tag="PLB46_17320"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRF78344.1"
                     /translation="VEHDAEVIWETQLETLREVLDIAHVSLDDIAGIGITNQRETVVV
                     WDKTTGKPIYNAIVWQDRRTAGICEDLKAGKLENYIRDNTGLVIDAYFSGTKIKWILD
                     TIPQARQLAAENKLLCGTIDCWLIWKLTNGNVHATDVSNASRTMLFNIKSITWDTHLL
                     QTLNIPANILPEVKNSIDQYGFANIGGKEIPILGVAGDQQAALFGQRCFEPGTAKNTY
                     GTGCFMLMQTGNKMVISQQKLLTTIAWGINGNISYALEGSVFIGGAVVQWLRDSLQII
                     SSSDESEYIAASLHDNGDVYFVPAFTGLGAPHWDMYAKGTISGLTRGTGRAHIIRAAL
                     ESIAYQTRDVLQAMNKDAGIILKTLQVDGGATANNWLMQFQSDILQTQVVRPEYLEST
                     ALGAASIAGIGAGLFSMEDLSTQPLTSTRFKPKMKLVEADELYFKWHTAVDRAKG"
BASE COUNT          477 a          245 c          387 g          457 t
ORIGIN      
        1 gtggaacatg atgctgaagt aatttgggaa acacaattgg aaacacttcg tgaagtattg
       61 gatattgcgc atgtttcgtt ggatgatatt gcaggaattg gtattaccaa tcagcgggag
      121 acggtggtag tgtgggataa aactacgggc aaacctattt ataacgccat tgtttggcaa
      181 gatagaagaa ctgccggtat ttgtgaggat ttaaaagctg gtaaattaga aaattatatt
      241 cgcgataata ccggactggt gattgatgct tatttttcgg gtacaaaaat taaatggatt
      301 ttagatacta ttccgcaagc aagacaatta gctgcagaaa ataaattatt atgcggcact
      361 attgattgtt ggctgatatg gaaattaact aatggtaatg tacacgccac tgatgttagt
      421 aatgcatcca gaaccatgtt gtttaatatt aaaagtatta cttgggatac acatttatta
      481 caaactttaa atataccggc caacatttta cctgaagtaa aaaattccat cgaccaatat
      541 ggttttgcca atattggcgg taaagaaatt ccgattttag gtgtggcggg tgatcagcag
      601 gctgccttat tcggacagcg atgttttgaa ccgggcactg caaaaaatac atatgggact
      661 ggctgtttta tgttgatgca aacaggcaat aaaatggtta tttcacaaca aaaattatta
      721 actacaattg cctggggaat aaatggtaat atcagttatg cattagaggg cagtgtattt
      781 attggtggtg cagttgtgca atggctgaga gatagtttac aaataatttc cagcagcgat
      841 gaatcggaat atattgctgc atcattacat gataatggtg atgtgtattt tgtccctgca
      901 tttaccggac ttggtgctcc gcattgggat atgtatgcaa agggaactat ttcgggatta
      961 acacgcggca caggcagagc acatattatt cgcgcagcat tggaatcgat tgcttatcaa
     1021 acacgggatg tgttgcaggc aatgaataaa gatgcaggta ttattttaaa aactttacaa
     1081 gttgatggtg gtgcaactgc taacaattgg ctgatgcaat ttcaaagtga tatattacaa
     1141 acgcaggtag tgcgccctga atatttagaa tcgacagcat taggtgcagc gagtatagcg
     1201 ggtattggag cggggttatt ttcgatggaa gatttgtcta cacagccgct tacgtctaca
     1261 cgatttaaac ctaaaatgaa attagtggaa gcggatgaat tgtattttaa atggcatact
     1321 gcagtggata gggccaaagg ataacggcgt ttgaaaagga acactggttg aacggattgg
     1381 ggacggattt tttttgagcc ttattggcgg cggacgagtc aagagtcgaa tattgatttc
     1441 aatggttcat ttgtaggatt aatttcaatg ctggcttcga attgcgttag ggatagtagc
     1501 ggaaaccccg caggccgcct tttcggcgga cgaggagttg cagcgtatag cccggccaac
     1561 gcccaa
//
LOCUS       DAORKW010000724         1563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5640_length_1563_cov_5.259947, whole genome shotgun sequence.
ACCESSION   DAORKW010000724 DAORKW010000000
VERSION     DAORKW010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5640_length_1563_cov_5.259947"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PLB46_17325"
     CDS             <1..562
                     /locus_tag="PLB46_17325"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRF78345.1"
                     /translation="YVGSVHPRKNVKNLLLAYDALRKEHLTEHQLVIVGRMAWKTDET
                     KQIFEQMQFKNDVVFTNHLLLSELTKVMASADAFVYPSLFEGFGIPIVEARYCGVPVI
                     TSDRSSLTEVGGPNALYFDPERVDLIKDAMKLFLLDRENFHAKAKKDTSVREVFNWDK
                     TVNSIESAIATMDSNFVKPRQSVEVA"
     gene            611..>1563
                     /locus_tag="PLB46_17330"
     CDS             611..>1563
                     /locus_tag="PLB46_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78346.1"
                     /translation="MKRLLLIVTLLTSYQIIQAQDKIKASEKLQLQKIGSVVLKDIHT
                     DFNPQLLALEMPAPGSESYRRHLIDLKEALYGNGKYVPTGVRLNDTALLSAPEVITGW
                     EGNIMGTGVPNDNDMAISNDGKIISVINSSIYIYNSDGSFLYSVSLSAFCDTLGNAYS
                     KFDPKVLYDPRADKFVLLFLAGFTPEQTNVIIAFSETNDPLGEWNFYELPGNPKNNDR
                     WTDFPMFALTEEELFLTINLIIPDEPWQTGFSETLIWQMSLDKGYTGDIIDAVYYDSI
                     FFAGNPIRNLNPVRGGSTTYGPDMYFLSNRNFAESNDTIFIV"
BASE COUNT          500 a          251 c          311 g          501 t
ORIGIN      
        1 atatgtcggc tcagttcatc cgagaaaaaa tgtaaaaaat ttattacttg cttacgatgc
       61 attgcgcaaa gaacatctca ccgaacatca attggtaatt gtgggacgaa tggcttggaa
      121 aactgatgaa accaaacaga tttttgagca aatgcaattt aaaaatgatg tagtgtttac
      181 caaccatttg ttactctcag aattaaccaa agtaatggcg tctgcagatg catttgtgta
      241 tccttcatta tttgaaggat ttggaattcc tattgttgag gccagatatt gcggtgttcc
      301 ggtgataact tcagaccgct ccagtttaac agaagtaggc ggaccaaatg cactttattt
      361 tgaccctgag cgggtagatt taataaaaga tgccatgaaa ttatttttgc tcgatagaga
      421 aaattttcat gctaaagcaa aaaaagatac atcagtgcga gaggtgttta attgggataa
      481 aacagtgaac agcattgagt cggcaattgc aaccatggac agcaattttg ttaagcccag
      541 gcaatcggta gaggtagctt aacagtttga ttaaaaggaa tactgtaaat tcgtagcaaa
      601 atttaccggt atgaaacgtt tactgctgat agtaacatta ttaacttctt atcaaattat
      661 ccaggctcag gataaaatta aggcttctga aaaactacaa ttgcaaaaaa ttggtagcgt
      721 agttttaaag gatattcata ccgattttaa tccgcaattg ctcgcattag aaatgcctgc
      781 tcccggttca gaaagttacc gtcgtcatct tattgattta aaggaagcac tatacgggaa
      841 cggtaaatat gtaccgaccg gtgtaagatt aaatgatacc gctttacttt cagctccgga
      901 agttataacc ggttgggagg gtaatattat gggtacaggt gtgcctaatg ataatgatat
      961 ggcaatttct aacgatggta aaattatttc agtaattaat tcttctattt atatttataa
     1021 tagtgatggc agttttttat attcagtttc attatccgca ttttgtgata ctctcggaaa
     1081 tgcgtatagt aaatttgacc ctaaagtatt atatgatccg cgtgcagata aatttgtgtt
     1141 gttatttctt gcaggattta cacctgaaca aacaaatgta attatcgctt tttctgaaac
     1201 gaatgaccca ctcggcgaat ggaattttta tgaattaccg ggtaacccga aaaataatga
     1261 caggtggaca gattttccca tgtttgcttt gactgaagag gaattatttc ttaccatcaa
     1321 cttaattatt cctgatgaac cttggcaaac aggatttagt gaaacactga tatggcaaat
     1381 gagtctcgat aaaggatata ccggagatat tattgatgct gtgtattacg acagtatttt
     1441 tttcgcagga aatcctattc gcaatttgaa tccggtaaga gggggttcta ctacctatgg
     1501 ccctgatatg tattttttat ctaacagaaa ttttgctgaa agtaacgata ctatttttat
     1561 tgt
//
LOCUS       DAORKW010000725         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5685_length_1556_cov_5.550966, whole genome shotgun sequence.
ACCESSION   DAORKW010000725 DAORKW010000000
VERSION     DAORKW010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5685_length_1556_cov_5.550966"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PLB46_17335"
     CDS             complement(<1..556)
                     /locus_tag="PLB46_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRF78347.1"
                     /translation="MKKTKILYVEDELNLGTIVTDSLEQKGFEVVLVKDGAKVMETFN
                     RFTPDLCVLDVMLPNIDGFELGRMINNRYPKLPIIFLTAKTQTQDVLTGFNSGATDYI
                     RKPFSIEELVARIHNQLQLARGANKYGEAITIGKFIFHSNKYELQFENTIYKLSNREA
                     EVLQVFCRNINNITDRKSLLLEVWG"
     gene            complement(553..>1556)
                     /locus_tag="PLB46_17340"
     CDS             complement(553..>1556)
                     /locus_tag="PLB46_17340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRF78348.1"
                     /translation="GFFTEILTAEDTTQLKSFFSANLIDNNISVKPVWVSGRKEKMFP
                     PPMFYYESHMLPSPYGVEIQQYNGYLVKALLPEIIFSLLLIGVITLAFIFSYRNIRNQ
                     MRLAVLKDDLISNISHELKTPVATVKVAIEALQHMNPQQQQEKMTNYLGMAAQEISRL
                     DLLVNKVMNSILLDNGKQVFQSEPIVLKNIIDEAILGMQMQVQQKGGVIQFLPGNVAG
                     NIVGDSLHIKGVIFNLIENSLKYGNEQPHINIQLIEEHGKVVITIADNGPGIPEAYTQ
                     KIFEKFFRVPSGNKHNIKGYGLGLYYAAQVMQQSGGSISVANNLTKGCTFTLVFPQA"
BASE COUNT          471 a          294 c          240 g          551 t
ORIGIN      
        1 caccccaaac ttctaacaac aaactttttc tatcggttat attattgata tttcggcaaa
       61 aaacttgcag cacttctgcc tcgcgattgc tgagtttata aatggtattt tcaaattgta
      121 attcatattt attactatga aaaataaatt ttccaatagt tatggcttct ccatatttat
      181 ttgcaccacg agcaagttgt agctgattat gaatacgtgc aacgagctct tcgatactaa
      241 atggtttgcg gatataatct gtagcgccgg aattaaatcc ggttaacaca tcctgtgttt
      301 gtgttttagc cgttaaaaat ataatcggca atttcggata tcggttatta atcattcggc
      361 caagttcaaa tccatctata ttgggcaaca taacatctaa cacacataaa tcaggtgtaa
      421 aacgattgaa agtttccatc actttagcac catccttaac cagtacgacc tcaaatcctt
      481 tttgctccaa actatcggtg acaatagtcc caagatttaa ttcatcctca acatataata
      541 tttttgtttt tttcatgctt gtggaaatac taatgtgaag gtacaacctt tggttaaatt
      601 attggcaaca ctgatacttc ctccgctttg ttgcattact tgtgctgcgt agtataatcc
      661 caggccataa cctttaatat tatgtttatt accggaaggt acgcggaaaa atttttcgaa
      721 tattttttgt gtataggctt cgggaattcc cgggccatta tctgcaatag ttatcacaac
      781 ttttccatgc tcttcaatca gttgtatatt tatatgtggc tgttcattgc catattttaa
      841 actgttttct attaaattaa atatcacccc tttaatatgt aaactatcac caactatatt
      901 acctgctaca ttacctggta aaaattgaat aacaccacct ttttgctgaa cttgcatctg
      961 catccctaaa attgcttcat caataatgtt ttttaacact ataggttcag attgaaatac
     1021 ttgtttgcca ttatccaaca aaatactatt cattactttg ttgaccaata aatcgaggcg
     1081 actaatttcc tgtgcagcca ttcctaaata attggtcatt ttttcctgtt gttgttgagg
     1141 gttcatgtgt tgtaatgctt cgatagcaac tttaactgtg gcaacaggtg tttttaattc
     1201 atgggaaata ttactaatca ggtcatcttt taaaactgcc aaacgcattt ggttgcgaat
     1261 attgcgatag gaaaaaataa atgccagtgt aattacacct attaataaaa ggctaaaaat
     1321 gatttccggc aataatgctt ttactaaata accattgtat tgttgaatct caacgccata
     1381 gggtgatggt aacatgtggg attcataata aaacattggt ggtggaaaca ttttttcttt
     1441 tcttcctgat acccaaaccg gttttacact gatgttgtta tcgatcaaat ttgctgaaaa
     1501 aaaacttttc aattgcgttg tatcttccgc agtaagtatc tcggtaaaaa atcccc
//
LOCUS       DAORKW010000726         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5687_length_1556_cov_4.434377, whole genome shotgun sequence.
ACCESSION   DAORKW010000726 DAORKW010000000
VERSION     DAORKW010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5687_length_1556_cov_4.434377"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLB46_17345"
     CDS             complement(<1..418)
                     /locus_tag="PLB46_17345"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRF78349.1"
                     /translation="MNRAKSTQLFEEAKQLFPGGVSSPVRSFKSVLGSPLFMKKGKGS
                     RVWDEDGNEFIDFCCSWGPLILGHADDRVVDAVAVALQHGTSFGTPTQYENILGKLII
                     ENHKYIEKLRFVSSGTEAVMSAIRLARGYTGKNKIIK"
     gene            complement(427..>1556)
                     /locus_tag="PLB46_17350"
     CDS             complement(427..>1556)
                     /locus_tag="PLB46_17350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRF78350.1"
                     /translation="DKGLVSKKMQPLAYFKMSCDKETWYIQTEKIGELPFLSAYVPVL
                     DDAGETMFYLNLPYFATEQNLRAEISSFMVTLVNVYVLLLILAGVIAFFVSKSITSPL
                     ATIAGKFKGIKLGKANEPIHWTHDDEIGLLVAEYNKMLQELENSAGLLAKSERESAWR
                     DMAKQVAHEIKNPLTPMRLSIQHLQRAIQTNAPNVKELTSKVTDTILEQIDNLTHIAS
                     EFSNFAVMPKAVNEQLCLNDLLENVTALFNEHDDVEITLSVPDNKIEVFADKNQLLRV
                     FNNVIKNAIQAIPDDRKGVIAITLQMAEHRALVKVADNGKGIPDYEKDKVFVPNFTTK
                     SSGMGIGLAMCKNIVESAGGYIWFESNEGEGTTFFIELPVI"
BASE COUNT          464 a          320 c          246 g          526 t
ORIGIN      
        1 atttgataat tttatttttt ccggtataac ctcttgccaa tcgaatagca ctcataaccg
       61 cctctgttcc tgatgaaaca aaacgaagtt tttctatgta tttatgattt tcaataatga
      121 gtttacccaa aatattttcg tattgcgtag gagtgccaaa tgaagtgcca tgttgtaatg
      181 ccacagcaac agcatctact accctgtcat ctgcatgacc taaaatgagt ggtccccatg
      241 aacaacaaaa atcaataaat tcatttccgt cttcatccca tacccggctg cctttacctt
      301 ttttcataaa taaaggagaa cctaatactg atttaaatga gcgcaccggt gaacttacac
      361 caccaggaaa taattgtttc gcttcctcaa ataattgtgt cgattttgct ctgttcattg
      421 atatcattat ataactggta attcaataaa aaatgtagtg ccttctcctt cattactttc
      481 gaaccaaata taacctcctg cactttcaac aatgttttta cacatggcta aacctatgcc
      541 catgcctgaa cttttggtgg taaaattagg aacaaatact ttgtcttttt cgtaatcggg
      601 tatacctttt ccattatcgg caaccttcac caaagcgcgg tgttcagcca tttgcaatgt
      661 aatcgcaatt acacctttcc tgtcatcggg aattgcctgt atggcatttt tgataacatt
      721 attaaatacg cgaagtaatt ggtttttatc agcaaatact tcaatcttat tatccggtac
      781 agaaagtgtt atttcaacat catcatgctc attaaaaagt gcggttacat tttcaagtaa
      841 atcgtttaaa cacaattgtt cgtttactgc ttttggcatt accgcgaagt tggaaaactc
      901 ggatgcgata tgtgttaaat tatcaatttg ttccaaaata gtatcagtaa cttttgatgt
      961 taactctttt acattaggtg cattagtttg aatagctctt tgtaaatgtt gaatacttaa
     1021 tcgcattggc gtcaatggat ttttaatttc gtgcgccact tgttttgcca tatcgcgcca
     1081 ggcactttct cgttccgatt ttgccaataa tccggcagag ttttctaatt cctgcaacat
     1141 tttattatac tccgctacca acaaaccaat ttcatcatca tgtgtccaat gaataggttc
     1201 attggcctta cccaatttga tacctttaaa ttttccggcg atagttgcaa gtgggctggt
     1261 aatacttttt gatacaaaaa acgcaattac accggctaaa atgagtaata atacatacac
     1321 gttaacaagg gtaaccatga aggaagaaat ttctgcccga aggttttgct cagtagcaaa
     1381 gtaaggcaaa tttaaataaa acattgtttc acctgcatca tccaataccg gaacatacgc
     1441 tgacaaaaat ggcaactcac caattttttc cgtctggatg taccatgttt ctttgtcgca
     1501 gctcattttg aaatatgcca atggttgcat ttttttagag acaagtcctt tatcaa
//
LOCUS       DAORKW010000727         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5818_length_1538_cov_4.722859, whole genome shotgun sequence.
ACCESSION   DAORKW010000727 DAORKW010000000
VERSION     DAORKW010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5818_length_1538_cov_4.722859"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PLB46_17355"
     CDS             <1..1270
                     /locus_tag="PLB46_17355"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HRF78351.1"
                     /translation="FALSSCSKIIIKYAGEADVQQTSLPPHEPMCKKSMSYIPDLRHL
                     NASPTKFVRVNFHIMQDSNGKGNFSEAQGKDFIRELVKQSNYRWANNQQMNLPEGNTT
                     DVIPIPVRIVLQKDPITGEDAIYFHKNDTLAFWNRSAKKGINSLGDRTVIDTYKTGED
                     SIINIFLIEHVPDSINSPTYGGATLSGISFVGSLKLFSMYNQFTTVKYRDDGTAFTHD
                     VLFLSKLLNHELGHSFGLNHTWNWDDGCEDTPRNPGCWSNTGKAPCDGIVSNNMMDYN
                     AIQMAVTPCQIGRMEYTFYKEKDGARQFTIPLWCEYHPFEKVTIFKHEQLVWEGGKDL
                     WGDIELREGSELTIRCEVALPEQAKVIIKPGAKLILDGGKLTNRCGEQFEGVEIWENK
                     KTGEKGQLFITNNGEITQVKNFVMIGPTFE"
     gene            1286..>1538
                     /locus_tag="PLB46_17360"
     CDS             1286..>1538
                     /locus_tag="PLB46_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78352.1"
                     /translation="MAYKQFTFLSPVTFIIALAGFFLTFTEINCNGQQLDTIKGIELV
                     TGYEQELDFNLGEDNSAEEKKVEKYDPNIFALNAFIAAII"
BASE COUNT          518 a          244 c          316 g          460 t
ORIGIN      
        1 atttgcacta agctcatgca gcaagatcat aataaaatat gcaggagaag ctgatgtgca
       61 gcaaacatct ttgccaccgc atgaaccgat gtgcaaaaaa tcgatgagtt atattcctga
      121 tttacgtcat ttaaatgcat ctcccacaaa atttgtccgg gtaaattttc atatcatgca
      181 ggatagtaat gggaaaggca attttagtga agcacagggg aaagatttta ttcgtgaatt
      241 agttaaacaa agtaattatc gttgggcgaa taatcagcaa atgaatttac cggaaggaaa
      301 tactacagat gttattccta ttcctgtcag aattgtttta caaaaagacc cgataacagg
      361 tgaagatgcc atttattttc acaaaaatga tacactggct ttttggaaca gaagcgcaaa
      421 aaaaggcata aattctttag gcgacagaac agtaattgat acttataaaa caggtgaaga
      481 ttccatcatc aatatctttt taattgaaca tgttccggat agtatcaata gtcctactta
      541 tggtggtgct accctatcgg ggatttcatt tgtaggcagc ttgaaattat tcagcatgta
      601 taatcaattt actactgtaa aataccgcga tgatggtact gcgtttacac atgatgtttt
      661 atttttatcc aaattactca atcatgagtt gggacatagt ttcggattaa atcacacctg
      721 gaattgggac gatggatgtg aagatactcc ccgcaatccc ggctgttgga gtaataccgg
      781 caaggcgcct tgcgatggta ttgtgagtaa taatatgatg gattacaatg ccattcaaat
      841 ggctgtaact ccttgtcaga ttggccgcat ggaatatact ttttataaag aaaaagatgg
      901 tgcacgccag tttactattc cgctgtggtg cgaatatcat ccattcgaaa aagtaaccat
      961 tttcaaacat gaacaattgg tttgggaagg tggaaaagat ttatggggag atattgaatt
     1021 gcgcgaaggt tcagaattga ccattcgttg tgaggtggct ttacctgagc aggcaaaggt
     1081 gataataaaa ccaggtgcaa aattaatttt agacggtggt aaattaacca accgttgtgg
     1141 tgaacaattt gaaggagttg aaatttggga aaataaaaaa accggagaaa aaggtcaatt
     1201 atttattacc aataacggag aaatcactca agtgaaaaac ttcgtaatga taggacctac
     1261 ttttgaataa tatttatttt aatttatggc atacaaacaa tttacatttt tatcgccggt
     1321 aacattcatt attgctttgg cgggtttctt tttaaccttt actgaaatta attgtaatgg
     1381 tcagcaactc gataccataa aaggtattga actggttacc ggatatgagc aggagcttga
     1441 ttttaattta ggagaggata acagcgccga agaaaaaaag gtggaaaaat acgaccctaa
     1501 tatattcgca ttaaatgctt ttattgccgc tataatcg
//
LOCUS       DAORKW010000728         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5819_length_1538_cov_4.285907, whole genome shotgun sequence.
ACCESSION   DAORKW010000728 DAORKW010000000
VERSION     DAORKW010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5819_length_1538_cov_4.285907"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1503)
                     /locus_tag="PLB46_17365"
     CDS             complement(205..1503)
                     /locus_tag="PLB46_17365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78353.1"
                     /translation="MRIITLITLCCLITSGLKAQIQCDNDSVGLVPLVDLKAGFYLGA
                     YQGGLYPSGKNFPPAGHIKKGLKIAKTIKPLDTLGNVNYETGKIVLAGFGGSTTGEPW
                     NHLIEITNFDATVNPCLKLLNATNSGEGMESMNVDHPDYWDYIEDTRIRPKGLTPAQV
                     QVAWLFNGSRADTIFDMPAYRDSIEKKVQLALAAMLIEYPNLKLVYVGSPYYAGYADP
                     TYEMYTSIHEPGSYRCGFGFKAAVEKQIMGDPMYKYTAPGKVVPFMLWGPYLWTDGDQ
                     PRTYDSLFWDCEDDFRVDGGGFHLNGEGKDKLALIIDNFFKTDTLAEIWYNDGPKWAS
                     CGDGRLADGSIISPTTIDNDEYQLYPTINNGIFTLELPSSNSLIQITITDNLGRIIYT
                     QNVIETEIQNLQLTNIPSGLYFCNIYYDNIHLVKEFIVNK"
BASE COUNT          493 a          288 c          247 g          510 t
ORIGIN      
        1 acgtttcatt ttttcttcga attttaattg ttatatgcac cacaaaggta gtgcttagct
       61 tgtttaacac caattttaaa taatggttcg gaaaagcata cgacatggtt cctgctgcca
      121 aattaataaa acctaattat caggtggtta aaaaatatgg ttccccaccc aataaaataa
      181 taatgtaagg ctagtgctaa aaagttattt attgactata aattctttca ctaaatgaat
      241 gttatcataa taaatattac aaaagtacaa gccacttgga atattggtaa gttgtaaatt
      301 ttgtatttct gtttctataa cattttgagt gtaaataatt cggcctaaat tatctgtaat
      361 ggtaatttgt atcaatgaat tgcttgatgg taattcgagt gtgaaaatgc cattattaat
      421 agttggatat aattgatatt catcgttatc aattgtggtc ggactaataa tactaccatc
      481 cgctaaacga ccatcaccac aacttgccca tttaggtccg tcgttatacc atatttcagc
      541 aagtgtatct gttttgaaaa aattatcaat aattaatgcg agtttatctt ttccttcacc
      601 atttaaatga aagcctccgc catcaactct gaagtcatct tcacaatccc aaaataaact
      661 atcatatgtg cgtggctgat caccatcggt ccacaagtaa ggcccccata acataaacgg
      721 cactacttta cctggcgcag tatatttata cataggatca cccataattt gtttttctac
      781 agcagcttta aatccaaatc cacaacggta agaacccggt tcgtgaatac tggtatacat
      841 ttcataagtc gggtcggcgt atcctgcata ataaggactt cctacataca ctaattttaa
      901 attgggatat tcaatcagca ttgctgcaag tgcaagttgt actttttttt ctatgctatc
      961 gcgatatgca ggcatatcaa aaatggtatc tgcacgtgag ccattaaata accaggcaac
     1021 ttgtacctga gcaggtgtta atccttttgg tctaattctg gtatcttcaa tataatccca
     1081 ataatcagga tggtcaacat tcatactttc cattccttct cctgaattgg tagcatttaa
     1141 taatttcaaa catggattta cagttgcgtc aaagttggta atttcaatta aatgattcca
     1201 aggttcaccg gttgttgagc caccaaagcc tgctaataca attttaccgg tttcataatt
     1261 tacattacct aatgtgtcga gcggttttat tgttttggca atttttaaac cttttttaat
     1321 atgccctgcg ggaggaaaat ttttaccaga aggatataag ccaccctggt aagcaccaag
     1381 gtaaaaaccc gcttttaaat ctaccagtgg cactaaacca actgaatcat tatcgcattg
     1441 aatttgtgcc tttaatccgc ttgtaataag gcaacataga gtaatcaggg tgatgatacg
     1501 catagggtaa ttttagtcaa atataaaaaa aatactat
//
LOCUS       DAORKW010000729         1536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5835_length_1536_cov_4.190412, whole genome shotgun sequence.
ACCESSION   DAORKW010000729 DAORKW010000000
VERSION     DAORKW010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5835_length_1536_cov_4.190412"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLB46_17370"
     CDS             <1..373
                     /locus_tag="PLB46_17370"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRF78354.1"
                     /translation="YEMVSRGCEDIVCVELDKRSIDFIRKTAAEINGNINVMKMDVFE
                     YIRTTKKKFDIIFAGPPYPLENIPALPEEIFEMQLLQPNGWFVLETNQKYKFDDHPHF
                     IRMRNYGTTHFHIFGAEPTSK"
     gene            390..854
                     /gene="coaD"
                     /locus_tag="PLB46_17375"
     CDS             390..854
                     /gene="coaD"
                     /locus_tag="PLB46_17375"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRF78355.1"
                     /translation="MTRIAVFPGSFDPVTLGHIDIIERAIPLFDKIIIAIGKNSQKQG
                     YFTLEQRLNWIQELYVHEQKITVDFYEGLTVNYCVEKNAGFIIRGLRSAADYEYEKVI
                     AQTNKTLNHQIETIFILSNPEFSHVSSTIVKEVLRNGGDISKMVPLIVTRGA"
     gene            complement(838..>1536)
                     /locus_tag="PLB46_17380"
     CDS             complement(838..>1536)
                     /locus_tag="PLB46_17380"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRF78356.1"
                     /translation="IVNNFSAKRVASLELKSMAQSYKIFINETPVYIIEGGLPATINT
                     GDAANPVFTSDQKKDIDKAFYLIENNAGIKSLTIYGHGIKAVKKSLFADYKTIRAAGG
                     LVFNNKDEVLMIFRRAMWDLPKGKIDLGEKKKAAALREVREETGLQKLSIVKKLRKTY
                     HTYRLENSTKVLKVTHWYLMMCSDNAQPIPQQEEDIEVATWIPAAEVDQKLNRAYANI
                     RDIITSGINTMHHG"
BASE COUNT          457 a          290 c          291 g          498 t
ORIGIN      
        1 ctacgaaatg gtttcacgag gctgtgagga cattgtttgt gttgaactgg acaaacgcag
       61 tatcgatttc atccgaaaaa cagcggctga aattaatggc aatatcaatg tgatgaagat
      121 ggatgttttc gaatatatca gaacaaccaa aaagaaattt gatatcattt ttgcaggacc
      181 tccttatcct ttggaaaata ttccggcttt acctgaagaa atttttgaga tgcaactcct
      241 ccagccaaat ggttggtttg ttttggaaac gaatcaaaaa tacaagttcg acgaccatcc
      301 tcattttatc agaatgcgga attacggtac gacccatttt catatttttg gagcggaacc
      361 tacaagcaaa taaatagtaa ctttactgta tgacacgaat tgcagtcttc ccgggatcat
      421 tcgacccagt tactttgggt cacatcgata ttattgaacg ggctatacca ttattcgata
      481 aaattatcat cgccatcggc aaaaacagtc aaaaacaagg ctatttcacc cttgaacagc
      541 gtttaaactg gattcaggaa ttgtatgtgc atgagcaaaa aattacagtg gatttttatg
      601 aggggctgac agtaaattat tgtgttgaaa aaaatgccgg attcatcatc agaggtttac
      661 gcagtgctgc tgattatgaa tatgaaaagg taatagctca aacaaacaag accttaaatc
      721 accaaattga aaccatattt atccttagta atccagagtt cagtcatgta agttcaacca
      781 ttgtcaaaga ggtgttacgc aatggtggtg atattagtaa aatggttcct ttgattgtta
      841 cccgtggtgc atagtattga taccactggt tataatatcg cgaatatttg catacgcacg
      901 atttagtttt tggtcaacct cagcagccgg aatccaggtt gctacttcaa tatcttcttc
      961 ctgctgcgga attggttgcg cattatcact gcacatcatc aaataccaat gtgttacctt
     1021 aagtacttta gtagaattct caaggcgata ggtatgatag gtttttctga gtttttttac
     1081 gatagacaat ttttgcagtc cggtttcttc tcttacttcg cgcaaagccg ctgctttctt
     1141 tttttcacct aaatcaattt tgcctttggg gagatcccac attgcacggc gaaaaatcat
     1201 gagcacttcg tctttattat taaaaactaa tccaccagcg gcgcgaattg ttttataatc
     1261 ggcgaataat gattttttta cagctttgat tccatggcca tagatagtta aacttttaat
     1321 cccggcattg ttttcaatta aataaaatgc cttgtcaatg tccttctttt ggtcagatgt
     1381 aaataccgga tttgctgcgt cgccggtatt aattgttgca ggtaatccac cttcaatgat
     1441 ataaaccggt gtctcgttga tgaaaatttt atagctttgg gccatgctct ttaattctag
     1501 tgacgcaact cttttcgctg aaaaattatt aactat
//
LOCUS       DAORKW010000730         1533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5852_length_1533_cov_3.850474, whole genome shotgun sequence.
ACCESSION   DAORKW010000730 DAORKW010000000
VERSION     DAORKW010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5852_length_1533_cov_3.850474"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLB46_17385"
     CDS             complement(<1..492)
                     /locus_tag="PLB46_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRF78357.1"
                     /translation="MMKIYCSTIVFLLMAFIANCQQYQIEIIPTNTPTSIRGLAALND
                     SIFWASGSNGYVGHSTDGGKSWQFKQIPGYDSAEFRDIEIINEKTILVMSSVQPACIL
                     KSTDKGNTWKKVYEDNRPEAFLDAMDFVGKKGICVGDPINGKFLVLQTTNKGDSWHIN
                     EQCS"
     gene            complement(489..1241)
                     /locus_tag="PLB46_17390"
     CDS             complement(489..1241)
                     /locus_tag="PLB46_17390"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HRF78358.1"
                     /translation="MKYFTSIIRLLSIAPLFVMAMSCSNKSTDNQNQTTKQVTYNRNT
                     FFGEIIYHSTIETADSNVAIAFKTLSPSIVHIYVDSNNFRLIETGGLSHGNVIIFKDA
                     KEVWQLDSIKKIAHLGEYSDFADPSSTLKNTMPDHYAPTVVTTNDSAVIAGHTCDKFR
                     ITRSGFIPQADTAYIWVARDIKFPSSRFDIQTEINRVAVPAPLYLGYEDGAILKFEVR
                     SKTNIRTMEVISLKENSFPQGIFVIPSNYQKK"
     gene            complement(1273..>1533)
                     /locus_tag="PLB46_17395"
     CDS             complement(1273..>1533)
                     /locus_tag="PLB46_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78359.1"
                     /translation="KFACNGISTQLTKSDIDDAVADTLNLVPALSSTIDGKFGDHKVF
                     YTVTTLKTSLYFYQLIGWTLYSYRNTVGTDLINASKSFAPVE"
BASE COUNT          476 a          272 c          279 g          506 t
ORIGIN      
        1 tgaacattgt tcattgatat gccagctatc acctttattt gtagtttgta atacaagaaa
       61 cttcccatta atcggatccc caacacaaat accttttttg ccaacaaaat ccatggcatc
      121 taaaaatgct tcaggtcgat tatcctcata cacttttttc caggtatttc ctttatctgt
      181 tgacttcaaa atacaagctg gctgcacact gctcattacg agaatagttt tttcgtttat
      241 tatttctata tcgcgaaatt cagcgctgtc gtaaccggga atctgtttaa actgccatga
      301 ttttccacca tccgtagaat gcccaacata tccattgctt ccacttgccc aaaaaataga
      361 atcgttgagt gctgctaatc cacgaatgct tgtgggtgta tttgttggta taatttcaat
      421 ttgatattgc tgacaattgg caataaaggc cattaataaa aaaacaattg tggagcagta
      481 aattttcatc attttttctg atagttggaa ggaattacaa atatgccctg aggaaacgaa
      541 ttttctttca ggcttattac ttccattgtt cggatattgg ttttacttct tacttcaaat
      601 tttaagatgg caccatcttc atatcctaaa tataaaggtg caggaacagc aacgcgatta
      661 atttctgttt gaatatcaaa tctgcttgag gggaatttaa tatcacgggc aacccaaatg
      721 taggcagtat cagcttgtgg aataaaacct gagcgtgtta ttctgaattt atcgcaggta
      781 tggcctgcaa ttacagcact gtcgttagtt gtaacaacag ttggtgcgta gtggtcaggc
      841 atggtatttt ttaatgtgct gcttgggtct gcaaagtcag aatattcgcc gagatgagct
      901 atttttttaa tggaatctaa ttgccaaact tctttagcat ctttaaaaat tatcacgtta
      961 ccatgcgaaa gtccaccagt ttcaattaat ctaaaattat tggaatctac ataaatatga
     1021 acaatggaag gactgagcgt tttaaaggca atagcaacat tagaatcagc tgtttcaata
     1081 gttgagtgat aaattatttc tccaaaaaaa gtattgcgat tataagttac ctgcttagtt
     1141 gtttgatttt gattgtcggt ggatttatta ctgcaactca ttgccattac aaacaatggt
     1201 gcaatagata gtaaccgaat aatagatgta aaatatttca ttacctgcaa agataaggaa
     1261 ggagagggta agttattcaa ccggagcaaa cgatttagag gcattgatca agtctgtccc
     1321 aactgtattg cggtagctat ataatgtcca gccgataagc tgataaaagt ataacgatgt
     1381 ttttaatgta gtaacggtat aaaatacttt gtgatctccg aatttgccat caattgtgct
     1441 ggaaagtgcc ggcacaagat ttaaagtgtc ggcaacagca tcatcaatat cactttttgt
     1501 taattgtgta ctaataccat tacacgcaaa ctt
//
LOCUS       DAORKW010000731         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5899_length_1525_cov_5.386395, whole genome shotgun sequence.
ACCESSION   DAORKW010000731 DAORKW010000000
VERSION     DAORKW010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5899_length_1525_cov_5.386395"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PLB46_17400"
     CDS             complement(<1..507)
                     /locus_tag="PLB46_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78360.1"
                     /translation="MNQLYRVFFVLGIMILVGYIRNRPQSGPEVVVKQKKLAEMVANA
                     DYDYLRTADPVSGNIPSGALMRAFYALKAKGYYQQDNSVLKTTEETGWQQINDFFPSL
                     AVTRITYDPSNTQVFYFCTGEGWFNADAVKGAGVFKSIDGGATWTQLASTANSNFDYC
                     QDIVVHPTN"
     gene            690..866
                     /locus_tag="PLB46_17405"
     CDS             690..866
                     /locus_tag="PLB46_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78361.1"
                     /translation="MLPFFIEHRNFSPDGKTWTIRLNLEPGKQYQYVLSTNFRDTQGN
                     RLKPYLLDFTTPPK"
     gene            969..1391
                     /locus_tag="PLB46_17410"
     CDS             969..1391
                     /locus_tag="PLB46_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78362.1"
                     /translation="MTKNVNALITKFSEQPKKLFLIDGIGALFTAILIGGILVQLSPL
                     IGLSKIILFQLAAIAILFALYSFSCNLYLKKVKPIFFQLIGYANLLYCGLTLLILLIN
                     CRQVQPLGYVYFFAEIVIIFVLSYIELSVANNLKIKGQ"
BASE COUNT          489 a          283 c          231 g          522 t
ORIGIN      
        1 attagttggg tgcaccacta tatcctggca ataatcaaaa ttactatttg cagttgatgc
       61 taattgtgtc caggttgcgc caccatcaat tgatttaaat actcctgctc ctttaacggc
      121 atcagcatta aaccaacctt ctcccgtaca gaagtaaaat acttgagtat ttgatgggtc
      181 gtaagtaatt cgggtgaccg ctaaacttgg aaaaaaatca tttatctgtt gccatcctgt
      241 ttcttcagtt gtttttagta cggaattatc ctgctggtaa taacctttag cttttaatgc
      301 ataaaatgcg cgcattaatg cacctgaagg aatattacct gaaacagggt cggcagttct
      361 caaataatca taatcggcat tggccaccat ttcggcgagt ttcttttgtt ttacaactac
      421 ctctggtcct gattgtggtc gattgcgaat ataccccacc aaaatcatta ttcccaatac
      481 aaaaaatacc cgatacaatt gattcatgcc atttatttga accctaaatt taagcattta
      541 taccaaaaaa ttcaaaatgc attaattcgt tattccgttc ccatcgaaaa tgcatgaaaa
      601 tccgctagta aaggtacttg caatcgaaag ggcaacatac gctcaattta ttttcatcaa
      661 cctgcacctt agtttgaaaa aattaataat tgcttccctt ttttattgaa catcgcaact
      721 tttctcctga tggcaaaaca tggactatca gactcaacct agaaccgggc aaacaatatc
      781 aatatgtatt atccactaat tttcgagata ctcagggcaa tagattaaaa ccttatttgt
      841 tggattttac aacacctccg aaataatttt taaattaatt taagattatt caattttaat
      901 caaacacata aatgttatga tatagcagaa cttcaattta tgtaatttta caggaattat
      961 ttcgacaaat gacaaaaaat gttaatgcgt taataactaa attttcggaa cagcccaaaa
     1021 aactattttt aatagatggt attggtgcct tgtttactgc aatacttatt ggtggtattt
     1081 tagtgcaact ttctccttta attggattat caaaaatcat attatttcaa ttagcagcaa
     1141 ttgctatcct tttcgcatta tattcctttt cttgtaattt gtatttgaaa aaggtaaaac
     1201 cgatattttt tcaacttatc ggatatgcca atttattgta ttgtggatta acgttactca
     1261 ttctcctaat taattgcaga caggttcaac ccttaggtta tgtttatttt tttgctgaaa
     1321 tagtgataat ttttgtttta agttatatag aattatcagt agctaataat ttgaaaataa
     1381 agggccaata acctaccaat tggtttagga tatcctaccg ggttgttcga gatacaccgt
     1441 tttaagtaac aaatgatgac atagcgcccc aacctaagag gcaactgact ggtaaaatca
     1501 gcctaggcta gtagtgcttt caccc
//
LOCUS       DAORKW010000732         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5918_length_1522_cov_5.555556, whole genome shotgun sequence.
ACCESSION   DAORKW010000732 DAORKW010000000
VERSION     DAORKW010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5918_length_1522_cov_5.555556"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1119)
                     /locus_tag="PLB46_17415"
     CDS             complement(13..1119)
                     /locus_tag="PLB46_17415"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRF78363.1"
                     /translation="MQLKQIGLLAICALLSASAIGQTTTPEQTFTPTIKVKALVHSRF
                     EASLTDSVDVQNKFSADPLTNNFRLRRVELRTDIKLNDKFSGVVRVQLPELKGTNPGR
                     VIELAYMDYKCSDYIQFRAGQFKMPYELDELTSHEDLRMIDRGTTSALFVSNSLSSYQ
                     PGAMLFGTFMKEKMPLNYYLAIANGSNRAVTNDDNSQKNLVGRLEFSPVKAIRIGANA
                     QNIYVKDTSGVSYGVDLSVQQKIGDKSKLIIEGEYIQGVNSTSFIADTTGLLIEDFNM
                     TGYFAQALLKVDINKSWCKTLEFGGKFENTDPNTTKDDNDYTTITGNIGFTFLPDNTA
                     RLQLNVINTNWASAISPGGITSANMFVAQFQLKI"
BASE COUNT          478 a          260 c          258 g          526 t
ORIGIN      
        1 taattgtcag cattaaattt taagttggaa ttgcgctaca aacatatttg ccgaagttat
       61 tcctccggga gaaatagcag aagcccaatt cgtgttgatt acgtttaatt gtaatcttgc
      121 ggtattatca ggtaaaaatg taaacccgat attacctgtt atagtagtat agtcgttatc
      181 gtctttggta gtattagggt cagtattttc gaacttacca ccaaactcta aagttttgca
      241 ccatgattta ttgatatcta cttttaataa agcctgagca aaataaccgg tcatattaaa
      301 atcttcaatc aacaaacctg ttgtatcagc aataaatgat gttgagttta caccttgaat
      361 gtattcacct tcgataatca attttgattt atcaccaatt ttttgctgca cgcttaaatc
      421 aacaccataa gaaacaccac tggtatcctt aacataaata ttttgcgcgt tggcaccaat
      481 tctaattgct tttaccggac taaattccaa acgcccaaca agattttttt gtgagttgtc
      541 gtcgtttgtc actgcacggt tactgccatt cgcaattgcg aggtaataat ttaacggcat
      601 tttttctttc ataaatgtac cgaacaacat ggcaccaggt tggtaggatg ataaactatt
      661 agacacaaat aatgcacttg tagttccgcg atcaatcata cgcaaatctt cgtggctagt
      721 taattcatct aattcataag gcattttaaa ttgacctgca cggaattgga tataatcgct
      781 gcatttataa tccatatatg ctaactcaat tacacgacca gggttggttc cttttaactc
      841 aggtaattgt acacgaacaa ctccgctgaa tttatcattt aatttaatat cggtacgcaa
      901 ttcgacacga cgtaatctga agttattggt taatggatca gcactgaatt tattttgaac
      961 atcaacgcta tcggttaatg aagcttcaaa tcgcgaatgc actaaagctt taaccttaat
     1021 ggttggtgta aaagtttgtt caggtgtggt agtttgacca attgcagatg cacttaataa
     1081 agcacatata gctaagagtc caatttgttt aagctgcata ttgtaagatt ttgtgcctca
     1141 aaggtattta tatggttttg caagtatatt aagccaatgt taagtttatt ttgtgtttat
     1201 gataagtttg caaatcgttt atgctgaatt aacatatggc ttgttttagc ccgattttaa
     1261 ggtatatgtt actataatta taacatatag ttaagtagat taccgcacag ttaaatgtat
     1321 aaataattga tatatgctga ttttagagga ttttttataa ttctgtagct atctcgctta
     1381 caggattaat tagttgtaaa aatcatcacc ccacttcgca aaggattaaa gtcagttgcc
     1441 cttttaaagt gaaaatcatc tgaaaacata atgtgcaaaa actttgggtt acaatttggc
     1501 atatcagggt ctaagcccta tt
//
LOCUS       DAORKW010000733         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_5994_length_1513_cov_3.727023, whole genome shotgun sequence.
ACCESSION   DAORKW010000733 DAORKW010000000
VERSION     DAORKW010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5994_length_1513_cov_3.727023"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLB46_17420"
     CDS             complement(<1..347)
                     /locus_tag="PLB46_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78364.1"
                     /translation="MKKFNLLLLLCICLIGNAKATIHIINVSNFVFTPNALTVNCNDT
                     IQFHRVNGTHPVISETGTWATFTMSGALINQNIVIATAGTYPYYCDFHGGAGGIGMAG
                     TITLTCAPPTCNTP"
     gene            complement(601..>1513)
                     /locus_tag="PLB46_17425"
     CDS             complement(601..>1513)
                     /locus_tag="PLB46_17425"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78365.1"
                     /translation="YIFCDYISGNFWTLSPDGLGGYETIFYDYVIDHITSFGENINGE
                     IFACINDNGNIYQVVDACQNFTATSSITHANAATINNGAIDITVSNGTAPYTFLWSNG
                     ATTQDISGLSAGTYTVDITDNIGCAITVVTEVENLCSEATGIISNPAATSVFINWDDV
                     GATGYRVMYKPVGPGPFSQINTPNSEINISGLNPSTPYTFKIRNKCPGAPGSFTANGN
                     FITLPLKREAIVEDTNVFPNPASDFITILNANEPTTLSIYSYNGELVGTYQINGESTI
                     NIGHLSNGIYQFYFQELAIAKTIIILH"
BASE COUNT          497 a          261 c          251 g          504 t
ORIGIN      
        1 ggggtattac aagtgggggg tgcacaggta agggtaattg ttcctgccat tccaattcca
       61 ccggctccac catggaaatc acaataatac ggataagtac cagccgtggc aataacaata
      121 ttttgattaa ttagtgcccc actcattgta aaagtagccc aggttcctgt ttcagaaatt
      181 acaggatgcg ttccatttac tcgatgaaat tgaatggtat cattacagtt tacggtaagc
      241 gcatttgggg taaaaacaaa attagacaca ttaataatgt gtattgttgc tttggcattg
      301 ccgattaaac aaatgcacaa caacaggagt aaattgaatt ttttcatttg agtattttag
      361 aatattaaaa ataaaactat ttaatcgaca ataccaacaa aataacatag ttattttttt
      421 attaattgtc tgccggagag cattcctatt acaaaataac agttgttaat aaatttctgt
      481 gcatcccgtt agacataggg tttacaataa atcagtttta cccaaaaaat gcaccacact
      541 aaatttgata tcaatttaat atatgcctat tatttaggat gcaaatagca tgataactat
      601 ttagtgtaaa ataattattg tttttgcaat tgccaattct tgaaaataaa actggtaaat
      661 accatttgat aaatgaccaa tatttattgt tgattcacca tttatctgat atgttccaac
      721 caattctcca ttgtagctat aaatactaag tgtagtgggt tcgtttgcat ttaaaattgt
      781 aataaaatca ctggcaggat tagggaaaac atttgtatct tcaactattg cctctctttt
      841 taaaggcaga gtaataaaat tcccattggc agtaaaactt ccaggtgcac ccggacattt
      901 atttcttatt ttaaatgtat aaggtgttga tggatttaat cctgaaatat taatttcgga
      961 attaggtgta tttatttgtg aaaacggtcc gggacccaca ggtttataca ttacccgata
     1021 tccggttgca ccaacatcat cccaattaat aaatacggat gtagcagctg gattactgat
     1081 tattccggtt gcttcactgc ataaattttc tacctcagta acaacagtaa ttgcacatcc
     1141 aatattgtct gtaatatcga ctgtatatgt gccggcgctt agccctgaaa tatcttgtgt
     1201 tgtcgctcca ttgctccata aaaaagtata tggtgccgtt ccattgctta ccgtaatatc
     1261 tattgcgcca ttattaattg tagctgcatt agcatgtgtt attgatgatg ttgctgtaaa
     1321 attttgacaa gcatcaacaa cctgataaat atttccgttg tcgttgatgc aggcaaaaat
     1381 ttcgccatta atattttcac caaaggaggt aatatggtct attacataat cgtaaaatat
     1441 tgtttcatat ccacctaaac catcgggtga taaggtccaa aaattgccac tgatataatc
     1501 acaaaaaata tat
//
LOCUS       DAORKW010000734         1508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6041_length_1508_cov_4.516173, whole genome shotgun sequence.
ACCESSION   DAORKW010000734 DAORKW010000000
VERSION     DAORKW010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6041_length_1508_cov_4.516173"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="PLB46_17430"
     CDS             complement(<1..1142)
                     /locus_tag="PLB46_17430"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRF78366.1"
                     /translation="MTKIFVSGLLLLVSTFLKAQSPELNCYMPDPDATIRSHNVDFKK
                     LDLDIKFTPEDGKLEGVAVYLFNALQVKVDSVFLDGPGITIQNITLNKQATRFKTDSA
                     GTTVFFNPPLVRNQDYMLQIQYDATPKRGIYFVGWNNSKTDDPNDPTIIRKQIWTQGQ
                     GVDNRHWIPSYDGLNDKLITALNITFDKNYTVVSNGALKNVIENSDGTKTWKYEMPHP
                     HALYLVMLAIGKYEYMDYTSKNGMISRQYYYPGTKHYAEQTYQYSAEMMDFLVDETGI
                     TYPWTTYANVPVQEFLYGAMENTTATIFSDFFYQDQRAFPDKNYVDINAHELTHQWFG
                     DYITAWSGSSHWLQESFATYYAKKFRQKISGDDYYHWKRREEMNTA"
     gene            complement(1212..>1508)
                     /locus_tag="PLB46_17435"
     CDS             complement(1212..>1508)
                     /locus_tag="PLB46_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRF78367.1"
                     /translation="TWNDLSNEPTQAAFEELDNGLKELLKIPYQVVAHKAAVRPTIKD
                     RTPVVGRHPAIENMYTLNGLGTKGTSLAPYYAKQLIDHILTGTPINHEVRVNRF"
BASE COUNT          449 a          276 c          278 g          505 t
ORIGIN      
        1 aaagcagtat tcatttcttc acgacgtttc cagtgataat aatcatcgcc actgattttt
       61 tgtctgaatt ttttagcata ataagtggca aaactttcct gcaaccaatg actggagccg
      121 ctccatgcag taatataatc gccgaaccat tgatgcgtta attcatgtgc attgatatct
      181 acataatttt tatccggaaa agctctttga tcctgataaa agaaatcgga aaaaatggta
      241 gcagtggtat tttccatcgc cccgtataaa aattcctgaa caggaacatt tgcataagtc
      301 gtccatggat aggtaatgcc agtttcatct accaaaaagt ccatcatttc tgcagaatac
      361 tgatatgttt gttcagcata atgttttgtc cccggataat aatattgtcg cgaaatcatt
      421 ccatttttag aagtgtaatc catatactca tactttccaa tagccagcat aactaaatat
      481 aatgcatgcg ggtgcggcat ttcgtatttc caagttttag tgccatctga attttcaatt
      541 acatttttta atgcaccatt ggaaactacc gtataatttt tatcgaatgt aatatttaat
      601 gccgtaatta atttatcatt taatccatca tacgaaggaa tccaatgtct gttatctaca
      661 ccttgtcctt gagtccaaat ttgtttgcga attatggtcg ggtcatttgg gtcatcagtt
      721 ttcgaattgt tccatcccac aaaataaata ccacgttttg gtgtagcgtc atattgaatt
      781 tgtaacatat aatcctgatt gcggactaat ggcggattaa aaaatacggt tgttcctgca
      841 ctgtcagttt tgaaacgtgt tgcctgttta ttgagtgtga tgttctgaat agtaattcca
      901 ggaccatcta aaaaaacaga atcaactttt acctgtaggg cattaaataa atagactgct
      961 acaccttcca atttcccatc ttccggagta aattttatat cgaggtctaa ctttttaaaa
     1021 tcgacattat gcgaccggat tgttgcgtca gggtctggca tataacaatt taactcggga
     1081 ctttgagcct ttaaaaaagt agatacgagc agcaataggc cactaacgaa tatttttgtc
     1141 atcctaaatg tttgaaaggt aaaaatagag tagtatggtt gatacacaaa attaatgaag
     1201 aattaggttc attaaaatct gttaacgcga acttcgtgat tgattggggt gcctgttaag
     1261 atgtggtcaa ttaattgttt tgcataataa ggtgctaacg aagttccttt agtacctaag
     1321 ccatttagcg tatacatatt ttcaattgcg ggatgtcgac ctaccactgg tgtccggtct
     1381 tttattgttg gccgcacagc agctttgtga gcaacaacct ggtatggaat ttttaaaagc
     1441 tcttttaaac cgttatcaag ttcctcaaaa gcagcctgtg taggttcgtt ggataggtcg
     1501 ttccaggt
//
LOCUS       DAORKW010000735         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6091_length_1501_cov_5.299447, whole genome shotgun sequence.
ACCESSION   DAORKW010000735 DAORKW010000000
VERSION     DAORKW010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6091_length_1501_cov_5.299447"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1501)
                     /locus_tag="PLB46_17440"
     CDS             complement(<1..>1501)
                     /locus_tag="PLB46_17440"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRF78368.1"
                     /translation="IGEYAIVKDTWKGMPVNYYVEKEYEKDAKAIFGNTPEMLEFYSN
                     LLGTKYVWPKYSQVVVRDYVSGAMENTTATIHGEFLQQHSRDLLDENYEDVISHELFH
                     QWFGDLVTTESWSNIPLNESFATYGEYLWREYKYGRDDADHAGLNDLNTYLDEAGYKQ
                     VDLIRFNYDTREDMFDSHSYAKGGRVLHMLRKYLGDEAFFAGLKKYLEDNKFSSVEMH
                     NLRLAFESVTGEDLNWFFNQWFFDNGHPDLTIYYDYFEADKTIAVTIYQIQDTSIAPI
                     YVLPMAVDIYANGKVERKQIVMDKAIQVFTFPTETRPDLINVDAEKMLLCTKMDNKSL
                     DEYLFQLQHAPLFLDRYEAVENLAAYQYENPAYRAAIENALNDKHWYIRQSALDTLLI
                     DESTSQAIKDKIMDMAKNDTRSYVRASALNKIGAIDGIDHMSIYNTALNDSSYTVVST
                     AMYAIYYLDKKAGLEIARKSKSIDNFSISYAVMTILGESGDVIDNDYFLN"
BASE COUNT          465 a          292 c          239 g          505 t
ORIGIN      
        1 tattgaggaa ataatcgttg tcaataacat ctccgctttc acctaaaatg gtcatcactg
       61 cataactgat actaaagttg tcgattgatt tacttttgcg tgcaatttcc aaacctgctt
      121 ttttatcaag ataataaatt gcatacatag cagttgaaac tactgtgtaa gaactatcat
      181 ttaacgctgt gttataaatt gacatgtgat caatgccgtc aattgcacct attttgttta
      241 aagcacttgc tctaacataa ctgcgtgtgt cgtttttagc catatccatt attttatctt
      301 taatggcttg tgaggttgac tcgtcaatta ataaagtatc aagggctgat tggcgaatgt
      361 accaatgttt atcgttcaaa gcattttcaa tagctgcgcg gtaggcagga ttttcatact
      421 ggtatgcagc aagattttca acagcttcgt aacgatcaag gaataatgga gcatgttgaa
      481 gttggaacag atattcatcc aatgatttgt tgtccatttt agtgcataac aacatttttt
      541 cagcatccac attaattaaa tcgggacgtg tttctgttgg aaatgtaaat acctgaatgg
      601 ctttatccat tacaatttgt ttgcgctcaa ctttgccatt agcataaatg tccacagcca
      661 taggcaatac ataaatcggt gcaattgagg tgtcttgaat ttgataaata gtaactgcta
      721 tagttttatc agcttcaaaa taatcataat aaatcgttaa atcaggatgg ccattgtcga
      781 aaaaccactg attaaaaaac cagtttaaat cttctccggt aacggattca aatgccaaac
      841 gtaaattatg catctcaaca gatgaaaact tattgtcttc taaatatttt tttaatccgg
      901 caaaaaatgc ttcatctcct aaatattttc tcagcatgtg taatacgcga ccacctttag
      961 cataagagtg cgaatcaaac atgtcctcac gagtatcgta attgaaacga attaaatcta
     1021 cctgcttata tcctgcttca tccaaatagg tattcaggtc gtttaatcct gcatggtctg
     1081 catcatcacg gccgtattta tattctctcc ataaatattc accataagtt gcaaacgatt
     1141 cgttgagtgg aatattactc cagctctcag tggttactaa atcgccaaac cattgatgga
     1201 ataattcgtg agaaattaca tcttcgtaat tttcatccaa caaatcgcgt gaatgttgct
     1261 gtaaaaattc accatgaata gttgccgtgg tattttccat agctccactt acataatcgc
     1321 gaactaccac ctgactatat tttggccaaa catatttagt acccagcaaa ttgctataaa
     1381 attccagcat ttcaggtgtg ttaccgaaaa ttgctttggc atctttttca tattcttttt
     1441 ctacataata atttaccggc atgcctttcc aagtatcttt tacaatagca tattcaccaa
     1501 t
//
LOCUS       DAORKW010000736         1479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6259_length_1479_cov_2.952247, whole genome shotgun sequence.
ACCESSION   DAORKW010000736 DAORKW010000000
VERSION     DAORKW010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6259_length_1479_cov_2.952247"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1479)
                     /locus_tag="PLB46_17445"
     CDS             complement(<1..>1479)
                     /locus_tag="PLB46_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HRF78369.1"
                     /translation="EEELTEFKLSGNHTAFWIPGDYDTNEYPYTKSRLSEVDAVAKSE
                     QHQEIATRTPIGKNYVQTPLMLKTDGGLYINIHEAALINYSAMQLRIDATTNTLTAAL
                     VPDAVGNKVYMNTPCVTPWRTIVVSDKATDILASKLILNLNAPSVIEDVSWIKPVKYI
                     GIWWDMHVGIAYWNYSNVTNINLATTDWSSLPANGRHGATTERAKYCIDFAAENGFDA
                     VLIEGWNVGWEDWFGKWKENVFDFVTPYPDFNVAELQQYAAAKNVKLIMHHETSASVT
                     NYERRLQEAMEFMLTYGYPAVKTGYVGRIIPRGEYHDGQWMVNHYNYVAAQMAKNKLM
                     LDAHEPVRPTGLHRTYPNWLACEAARGNEFNAWSQGNPPEHETILPFTRLMGGPMDYT
                     PGIFEIKMNYYQPASNEQVHTTLAKQLALYLTMYSPLQMAADLPENYLKYPDAFQFIK
                     DVAVDWQDTKYLEAEPGDYITVARKAKNSRKWFVGAINDEKKR"
BASE COUNT          404 a          315 c          260 g          500 t
ORIGIN      
        1 cctttttttt tcatcgttta ttgcaccaac aaaccatttc ctgctatttt ttgctttcct
       61 ggcaacagtg atataatcac cgggttctgc ttctaaatat ttggtatcct gccaatccac
      121 cgccacatct ttaataaact gaaatgcatc aggatatttt aaataatttt caggtaaatc
      181 ggctgccatt tgtaacggtg aatacatggt gagatataat gccaactgtt tcgctaatgt
      241 tgtgtgtacc tgctcattgg atgccggttg gtaataattc atttttattt caaaaatacc
      301 gggtgtgtaa tccattggcc cgcccattaa tcttgtaaaa ggcaaaatgg tttcatgttc
      361 aggcggattg ccctggctcc aagcattaaa ttcgttacct cgcgcagctt cacaagctaa
      421 ccagtttgga tatgtgcgat gtaaaccggt tggccgaaca ggttcatgtg catcgagcat
      481 caatttattt tttgccattt gtgctgcaac ataattgtaa tgatttacca tccattgtcc
      541 atcgtgatat tcacctcttg gaattattct tcctacatat cctgttttaa ctgcaggata
      601 cccataagtt aacataaatt ccatggcttc ctgcaatctg cgttcgtaat tcgttaccga
      661 agcggaggtt tcatgatgca taattaattt aacattttta gctgcagcat attgttgtaa
      721 ttccgcgaca ttaaaatcgg gatatggtgt tacaaaatca aaaacatttt ctttccattt
      781 tccaaaccaa tcttcccaac ctacattcca tccttcaatt aataccgcat caaatccatt
      841 ttcagcagca aaatcgatac aatattttgc gcgttcagtg gtagcgccat gtcttccgtt
      901 ggcaggaagg ctgctccagt ccgtggtggc aaggtttata ttagttacat tcgaataatt
      961 ccagtatgca ataccaacat gcatatccca ccaaatacca atatacttca cgggtttaat
     1021 ccagcttaca tcttcaataa cagatggagc atttaaattt aatatgagtt ttgaagccaa
     1081 aatatctgtt gctttatcgc ttaccacaat agtgcgccaa ggtgttacac aaggtgtgtt
     1141 catgtaaact ttattgccaa cagcatcggg cactaatgca gcagtcaacg tatttgtagt
     1201 cgcatcaata cgcaattgca tagcgctata attaattaaa gctgcttcat gaatattgat
     1261 atacaatccg ccatctgttt taagcatgag cggcgtttgc acataatttt ttccgatggg
     1321 tgttctggta gcaatttcct gatgttgttc cgattttgct accgcatcta cttcacttaa
     1381 tctgctttta gtgtaaggat attcattggt atcataatca cctggaatcc aaaatgcggt
     1441 gtggttgccg cttaatttaa attcggtaag ttcttcttc
//
LOCUS       DAORKW010000737         1470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6334_length_1470_cov_3.850883, whole genome shotgun sequence.
ACCESSION   DAORKW010000737 DAORKW010000000
VERSION     DAORKW010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6334_length_1470_cov_3.850883"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..202)
                     /locus_tag="PLB46_17450"
     CDS             complement(11..202)
                     /locus_tag="PLB46_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRF78370.1"
                     /translation="MNTGTVKFYNESKGFGFIKDESTQKEYFVHASGLIDKIRENDNV
                     VYDLQEGRKGLNAVDVKLA"
     gene            631..1230
                     /locus_tag="PLB46_17455"
     CDS             631..1230
                     /locus_tag="PLB46_17455"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRF78371.1"
                     /translation="MKKMFQLMFALVVMVSTTFSAKAQEYTPGSFTISAGYGFPSITK
                     TIFNIVDGDKISSSFYGPFYGKAEYKINETVGFGVNFAYASGTSTYLAQDEEIDTLFY
                     DGAVKYNAYSILARFNFHFGNSEVFDPYAGIGLGYRHNNYSYSGGDPDNQPLDVSGLL
                     NTGLDFTIGARFYLTENIGLYGEVGIAKTPIQVGLVVTL"
BASE COUNT          451 a          307 c          250 g          462 t
ORIGIN      
        1 caggtattat ttaggcaagt tttacgtcca ctgcattcaa tccttttctg ccttcctgaa
       61 ggtcataaac aacattgtcg ttttcgcgaa ttttatcgat caggcctgag gcatgtacga
      121 aatattcttt ttgtgttgat tcatctttaa tgaaaccaaa tccttttgat tcattataga
      181 attttactgt tcctgtattc attgtaattt ttaatttatg taaaggtaga catatttacc
      241 acataaacgc ccttttttgt aaataatcgt cgttttggaa acgctaaacc taaatccgga
      301 catacatctg tctaagaaaa gcctaattta tgaaaaacga tatgtgcaaa aaaatattta
      361 aaatattttt ataatggcaa atattgttat aaataatgca ataaatgtaa gtatttatgc
      421 aaatatttag taatcgagtt atttgatgct attgcattcc agccaattag ggtgcaaaaa
      481 aataaattaa gaaatttttg gcttcaatag tccaattcct gcaaaaagca aagcaagctc
      541 aggttttggc ataacgattt ggcataacac ttggttaatg cagactaaca catgcgatat
      601 tggatgtgtt aggcaaaaaa atttactgct atgaaaaaga tgtttcaatt aatgtttgct
      661 ttggtagtta tggtttctac cacattttca gcaaaagcac aggagtatac tccgggttcc
      721 ttcaccattt cagccggtta tggttttcca agtattacaa aaaccatatt taacattgta
      781 gatggcgaca agatttcatc cagcttttac ggaccttttt acggaaaagc cgagtataaa
      841 atcaacgaaa ccgttggctt tggtgtcaat tttgcctacg catccggaac atccacctat
      901 ttagcgcagg atgaagaaat agataccctg ttttacgatg gcgcggtgaa atacaatgcc
      961 tacagcatct tagcccgttt caacttccat tttgggaata gcgaagtgtt tgacccttac
     1021 gccggtatcg gtttaggtta tcgtcacaac aactatagtt attccggtgg cgaccccgac
     1081 aatcaaccat tagacgtgag cggtttatta aacaccggcc tcgatttcac catcggtgcc
     1141 cgcttttacc tcaccgaaaa cataggtctt tacggtgaag tcggcattgc caaaacacct
     1201 attcaggtag gactggtagt cactttataa gcaaccaccg cttaataaaa atgaaatata
     1261 taacaccaaa aggctgtcgc actggcagcc ttttttattg ccttttcatc aagtccaaac
     1321 attacatttt cacaatttgg agcatcacaa tcccgctccg ccaaatcaat ccgaaccggc
     1381 tattcgctgc aacaaaccgc aattcttttc ataactcaaa tcccgctgtt tccccctcag
     1441 tggacgcttc gccaatcagc acattatctt
//
LOCUS       DAORKW010000738         1465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6378_length_1465_cov_3.060993, whole genome shotgun sequence.
ACCESSION   DAORKW010000738 DAORKW010000000
VERSION     DAORKW010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6378_length_1465_cov_3.060993"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="PLB46_17460"
     CDS             complement(<1..1299)
                     /locus_tag="PLB46_17460"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HRF78372.1"
                     /translation="MATLLFMLGSHVMYAQEPVAMKVVPTINYLPSTVYYVTTDANQF
                     IWIGSNTGVYKYDGNNFTHYTSADGLGDNEILRIYEDKKGRIWFQSVNGNPSFYLDGK
                     IYNASNSDLVKQLKFKKMILTECEDDNGNLYIGSRQQIYYKIDSNDVVTKFEYDGQEN
                     FAWIGADNQIKYLKYRFQNTFQAIRGNALDDLIYIGQQLTLYRIENDSILKVQFQLPE
                     EAQEIIFVRIKSPNEIYLGTRNGLYVYYPNTTKPLRHFMAGLSISSVVFDLEDNLWVS
                     TLEAGVYLIPSLEVNIINSKNGLPEDKITCIEHDSKSNLWVGMAKNNFSIINLRGEIK
                     NNVLSATSSHDVTNIRHFGETTYIIGKSNIAKVTEGVTKSYGIYGNDIFFTKSDTVYI
                     AQDNTIAVDLDLFENHIHEVVYNMATKRKLYEFLNARTNVL"
BASE COUNT          444 a          276 c          223 g          522 t
ORIGIN      
        1 taatacattg gtacgggcat ttaagaactc atacaacttg cgtttggtgg ccatattgta
       61 aaccacttca tggatatggt tttcaaacaa atccaggtct actgcgatgg tattatcctg
      121 tgcaatataa acagtatcac tttttgtaaa aaaaatatcg ttgccataaa taccgtacga
      181 ctttgtaact ccttcggtaa ctttagcaat attacttttt ccaataatgt atgttgtttc
      241 accaaaatgt cgaatattgg taacatcatg tgacgaagtc gcacttaaca cattattttt
      301 aatttcacct cgcagattga taatactgaa gttgtttttt gccattccta cccataggtt
      361 actttttgaa tcgtgttcaa tacaggtaat tttatcttca ggtaaaccat tttttgaatt
      421 aattatattt acttctagtg atggaattaa atatacgcca gcttccagtg ttgaaaccca
      481 caagttatct tctaaatcaa aaactacact ggatatagat aatcctgcca taaagtggcg
      541 taatggcttt gtggtgttcg gataataaac atataaaccg ttgcgtgtac ctaaatatat
      601 ttcattaggg gatttaattc gcacaaaaat tatttcctgt gcttcttccg gtaattgaaa
      661 ttgtactttt aaaatactgt cattttcaat cctgtataaa gtaagttgct ggccaatata
      721 aattaaatca tccaatgcat ttcctctaat tgcctgaaat gtattttgga accgatattt
      781 caaatacttt atttggttat cagcgccaat ccatgcaaag ttttcctgac catcatattc
      841 gaattttgtt acaacatcat tggagtcaat tttataataa atttgttgtc gtgaacctat
      901 gtataaatta ccattgtcat cttcacattc agttaaaatc attttcttaa attttaactg
      961 ctttaccaga tcgctgttgc ttgcattata tatttttccg tctaaataaa aagagggatt
     1021 tccattaaca gactggaacc aaattctccc ttttttgtcc tcatatatgc ggagaatttc
     1081 attatcgcct aagccgtccg cactggtata atgggtgaag ttatttccgt catatttata
     1141 tactcctgta ttgctgccta tccaaataaa ttgattggca tcagtagtta cataatatac
     1201 cgttgatggt agatagttta tagttggaac aactttcatt gcaaccggct cctgtgcata
     1261 cataacatgc gaccccagca taaatagcag ggtagccaat aaccatatat tttttccggt
     1321 atgctgaagc atgcgctgtg atgaaactcc gcattaaaat taccgatata tttatatatc
     1381 tacctgcttt gactgcgaca gttgtccttt aaatgtcccc acttataaca caaatgcctt
     1441 caactgttta tcaattgaag gcatt
//
LOCUS       DAORKW010000739         1459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6426_length_1459_cov_4.151709, whole genome shotgun sequence.
ACCESSION   DAORKW010000739 DAORKW010000000
VERSION     DAORKW010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6426_length_1459_cov_4.151709"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..>1459)
                     /locus_tag="PLB46_17465"
     CDS             complement(71..>1459)
                     /locus_tag="PLB46_17465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78373.1"
                     /translation="IYLGGTELAEVNLNAITMPAKSNYGLYLDASTDYGVEENYFTGS
                     GATNPIGQTGIVINDNGTSNNSIYRNAFTDIAYGIKAQNSNGNTTSGIFFKCNTFDEN
                     KYDIAVTSGGIKKDQGQCVFENVSTPAGNVFTDNLTTSTLQFAANSGVDEIIYHHHND
                     ASAATAPFYEPYEFSSSIISLDECLEDYNALESCPVDFSDGGGSRLVNSGPRGVVDYN
                     LNSKEIKDTLSQFLADIERNKLIRNYLDSEQIAELSTFLSADSSSRAASLNAVIAMKQ
                     GNYEAASSNIQLIDENINSEALGYSNELYSIVNELTQDTLSWFDMNAEQLEKIKLLST
                     INNRIGVVAKLILEEISSVPYEEPLEEIIIESFVDNKENSGITREVTIQIYPHPVGSQ
                     STLQISLSQPSQNSKCFIFNLLGQEMLTFDLFNEHNNFNINNNMLPSGVFIAVIYDGN
                     KFVDSFKFIVGE"
BASE COUNT          466 a          265 c          214 g          514 t
ORIGIN      
        1 actctgcccg ttcagactac taaaataagt aaaaaacata ccaaatagac atccgaccca
       61 caatattatt ttattcacct acaataaatt taaatgaatc aacaaatttg tttccatcat
      121 aaattacagc tataaatacc cctgatggaa gcatgttatt attaatatta aaattgttat
      181 gctcattaaa taaatcaaat gtcaacattt cttgccctaa taaattaaaa ataaagcatt
      241 ttgagttttg gctaggttgt gaaagcgaaa tttgcaaggt gctttgtgag ccaaccggat
      301 gtgggtagat ttgaattgtt acctctctgg tgattccaga attctcttta ttatccacaa
      361 atgattctat aatgatttct tctagtggtt cctcgtatgg tacacttgaa atttcttcta
      421 gaataagttt agcaacaaca cctattctat tatttatagt tgataatagt tttatttttt
      481 ctaattgttc tgcattcata tcgaaccaac ttagggtatc ttgagttaat tcgtttacta
      541 ttgaataaag ctcattagag tagcccaatg cctcgctatt tatattttca tcaattaatt
      601 gaatgttaga gctagctgct tcataattac cctgtttcat agcgattaca gcatttaaac
      661 tagccgctct tgaactacta tctgccgata aaaatgtgct tagttcagca atttgttctg
      721 agtctaaata atttctaata agtttatttc tttcaatatc ggctaaaaac tgcgataaag
      781 tatcttttat ttctttggaa tttaaattgt aatcaaccac gcctcgtgga cccgaattga
      841 ccaatcttga accacctccg tcagaaaaat ctacagggca agattctaaa gcgttataat
      901 cttccaaaca ttcatcaagt gaaattattg aagaagaaaa ctcataaggt tcataaaatg
      961 gtgcagtagc cgcacttgca tcattatgat gatgataaat gatttcatcc acgcctgagt
     1021 tagctgcaaa ttgtaatgtt gaagttgtca aattatcggt aaaaacattg cctgccggag
     1081 ttgatacatt ttcaaataca cactgaccct gatccttttt tataccacct gatgtaactg
     1141 caatatcata tttattttca tcaaatgtat tacatttaaa aaatataccc gaagttgtat
     1201 tcccattact attttgcgct ttaattccat atgcaatatc ggtaaaggca ttacgataaa
     1261 tgctattatt ggaggtgccg ttatcattta taacaatacc tgtttgtcca attggattag
     1321 ttgcgcctga acctgtaaaa taattctctt ccactccata atctgttgag gcgtcaaggt
     1381 ataaaccata atttgatttt gcaggcattg ttatggcatt tagatttact tctgctaact
     1441 ctgttccgcc aagatatat
//
LOCUS       DAORKW010000740         1450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6505_length_1450_cov_3.442294, whole genome shotgun sequence.
ACCESSION   DAORKW010000740 DAORKW010000000
VERSION     DAORKW010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6505_length_1450_cov_3.442294"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..718)
                     /locus_tag="PLB46_17470"
     CDS             complement(125..718)
                     /locus_tag="PLB46_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78374.1"
                     /translation="MKRVIQLTVIGNTKIFILVLLLVFLQAVVSAQDTTAKKPNTIYI
                     EGLGIGLRYSINYERQFQIKESHWGFSLSGGVAPNFVSPLYLYFPLRFDIDYQFGNSK
                     AGIGFNYMWGFYWQPYHGSYDDLIRSTDFASFPKIKYQYLCRNGLQLTGELNMISEKW
                     QPHQNVISYLVDLPFGKLELYQKQFVAWPGISIGYTF"
     gene            complement(844..1311)
                     /locus_tag="PLB46_17475"
     CDS             complement(844..1311)
                     /locus_tag="PLB46_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78375.1"
                     /translation="MSYNLLLFNKNTAPKTRAEFMQWYEVQTQWTEEHSYDDPAITSV
                     ELRNWFMEVIQTFPAMNGPFASEDEDNIYVTDYSIGKDIIYVSIAWSIAEQAHIKMLA
                     LAEKYGVGFFEASNDNGAIYFPENGKLTPVDNPANKTKNASDKQKIKPWWKFW"
BASE COUNT          453 a          251 c          243 g          503 t
ORIGIN      
        1 ggagagatgg agatggatgg ttgacgtatt tgttaattaa atgaagttgg ttaattagca
       61 ataaatatga cttttaaaca agtgctgact tttgaattgg tgttgtatta agcacttgtt
      121 gtttttaaaa agtatatcca attgaaatac ccggccaagc aacaaattgt ttttgatata
      181 attccaattt accaaatggt aaatccacaa gataggaaat gacattttga tgaggttgcc
      241 atttttctga tatcatattt aattcaccag taagctgtaa accattccta cataaatact
      301 ggtatttaat tttggggaaa gatgcaaaat ctgttgaccg tattaggtca tcgtaagatc
      361 catgataagg ttgccaataa aaaccccaca tatagttaaa tccaatacct gcttttgaat
      421 taccaaactg ataatcaata tcaaatcgca aagggaaata caaataaagc ggtgaaacaa
      481 aattaggtgc aacacccccc gataatgaaa atccccaatg tgattcttta atttgaaact
      541 gtctttcata attaatcgag taacgcaacc cgatacctaa tccttctata taaattgtat
      601 taggtttttt ggctgttgtg tcttgagcac ttactactgc ttgtagaaaa acaagtaata
      661 acactaaaat aaaaatttta gtattaccta taacagttaa ttgtataacc cttttcattt
      721 aataaactta ttggttggat atggcaattt caataccgtt taattacatt ttagttgtaa
      781 tccttttatg aatttgctca ctgcgctaat tctgggtttt gattgccaag tcgtgtttca
      841 aatttaccaa aatttccacc aaggtttgat tttttgtttg tctgaagcat ttttagtttt
      901 attggctgga ttgtctactg gtgttaattt tccattttca ggaaaataaa tggctccgtt
      961 gtcattagaa gcctcaaaga aaccaactcc gtatttttct gctagtgcta acatcttaat
     1021 atgcgcctgt tcagctattg accaggcaat tgaaacataa ataatatcct ttcctatgct
     1081 gtagtcagtt acatatatat tgtcttcatc ttcacttgca aacggaccat tcattgccgg
     1141 aaaagtctgt atcacttcca taaaccaatt gcgcaattca accgaagtaa tcgccgggtc
     1201 gtcataacta tgttcttcag tccattgggt ttgtacttca taccattgca taaattcagc
     1261 tctagttttt ggagctgtgt ttttgttaaa taacagtagg ttgtaactca tgttttgggt
     1321 ttttgatggg ttttataggt ttaacgtttt gcagctaccc gaaggaggcg gtttcgaagc
     1381 aattcacttt caacaaagca caaactttga tagaagcaca aagcttgatt taaccactga
     1441 accgccactt
//
LOCUS       DAORKW010000741         1440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6591_length_1440_cov_3.646931, whole genome shotgun sequence.
ACCESSION   DAORKW010000741 DAORKW010000000
VERSION     DAORKW010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6591_length_1440_cov_3.646931"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1440)
                     /locus_tag="PLB46_17480"
     CDS             complement(<1..>1440)
                     /locus_tag="PLB46_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78376.1"
                     /translation="LYYLRERITHKHLEVKHLVATNINEWFIFDATLFDRLFAQNKNL
                     VKQFTDFEDGRLADTKTDFFYKQIAEPFIESITGEITFTYFNSQDFQKPLRNAAKADD
                     NALIALFKLLSPEHLLKLPFTNDSNSLDKRFYSELLHIIGLTETKEGGKKLIERHKAG
                     ERHSGTILEDTIIQLDSLDKLSRLEKPNQFGANQNERLFNVGLELSITWINRILFLKL
                     LEAQLITYHKGDANYSFLNLDKIKNYDDLNSLFFQVLARKYDERNDDVKKVFAQVPYL
                     NSSLFEPTDLEQVTLFISNLKDDKTIPVISSTVLKDQHGKKLTGNLKTLEYLFAFLNA
                     YDFSSEGSEEIQEDNKTLINASVLGLIFEKINGYKDGSFFTPGFITMYMCRETIRKAV
                     VQKFNEVKNWNCTSIDDLYDKIEDRADANNIVNSIKICDPAVGSGHFLVSALNEMIAV
                     KNDLNILQDRTGKRLKEYNVEVVNDELIVT"
BASE COUNT          436 a          271 c          241 g          492 t
ORIGIN      
        1 gtgacaatta attcgtcatt tactacttcc acattgtatt ccttcaaacg tttaccggtt
       61 ctatcctgca gtatgtttag atcgttttta acggcaatca tttcattgag ggcagaaacc
      121 aaaaagtgtc ctgagccaac tgccgggtcg caaattttaa tactgtttac aatgttgttt
      181 gcatctgcac gatcctcaat tttatcgtat aggtcatcaa tacttgtgca attccagttt
      241 ttaacttcat taaatttctg cactactgcc ttcctgatgg tttcgcggca catatacatg
      301 gtaataaagc ccggtgtaaa aaacgaacca tctttatatc cgtttatttt ttcgaatatt
      361 aaacctaaaa cagaggcatt aattaatgtt ttattatcct cctgaatttc ttcggaccct
      421 tcactgctaa aatcataagc atttaaaaag gcaaataaat attccagcgt tttcaaattg
      481 ccggtaagtt ttttgccatg ttggtccttt agcaccgtag aggatattac cggaatagtt
      541 ttatcatcct tcagattact gataaacaat gtaacctgtt caaggtctgt tggttcaaat
      601 agtgaagagt tgaggtaagg cacttgtgca aatacctttt ttacatcatc gttacgctca
      661 tcatatttac gagccaatac ctgaaaaaac aaactattca ggtcatcgta attttttatt
      721 ttgtcgagat taaggaacga gtaattagca tcacctttgt gataagtgat caactgcgct
      781 tcaagtaatt ttagaaataa aatcctgttt atccaggtaa tggataactc caatccaaca
      841 ttaaacagtc gttcattttg attagcacca aattggttag gtttttcaag cctgctcagc
      901 ttatcgagac tatccaattg aataatggta tcttccaata tggtccctga atggcgctca
      961 ccagctttat ggcgttcaat caatttttta ccaccttcct ttgtttctgt aagaccgata
     1021 atatgtaata attcgctgta aaaacgtttg tcgagactat tactatcgtt agtaaatggc
     1081 agttttaaaa ggtgttcagg agaaagtaat ttaaataaag caatgagtgc attatcgtcg
     1141 gctttagctg cattgcgcag tggcttttga aaatcctgac tattaaaata ggtaaatgtg
     1201 atttcgccgg tgatactttc aataaacggt tcagctattt gtttgtaaaa gaaatccgtt
     1261 ttggtatcag caaggcgtcc atcttcaaaa tctgtaaact gttttaccag atttttattt
     1321 tgtgcaaaca agcggtcaaa taatgtagca tcaaaaataa accactcatt aatattggta
     1381 gcaactaagt gttttacctc taaatgttta tgcgtaatcc gctcccgtaa ataatacaac
//
LOCUS       DAORKW010000742         1435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6629_length_1435_cov_4.255797, whole genome shotgun sequence.
ACCESSION   DAORKW010000742 DAORKW010000000
VERSION     DAORKW010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6629_length_1435_cov_4.255797"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..580)
                     /locus_tag="PLB46_17485"
     CDS             complement(44..580)
                     /locus_tag="PLB46_17485"
                     /inference="COORDINATES: protein motif:HMM:NF015608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRF78377.1"
                     /translation="MAYYSYFVAAHIIFIVTWFAALFYIVRLYIYHTEALQRNEPDKS
                     ILATQFTIMERRLMSIIGTPSMILVVITGSSLLYFNQQFLQEGWMHMKLFFILCVITY
                     HFSCLRIQKRLSNKTSKLSSQQLRLFNELATIFLVAIVFLIELKNTANMLYGLLGLLL
                     LAVIMMIAVKIYKRKREA"
     gene            complement(598..>1435)
                     /locus_tag="PLB46_17490"
     CDS             complement(598..>1435)
                     /locus_tag="PLB46_17490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HRF78378.1"
                     /translation="WELAAGIVDTGDDLRTKTVTDWPGPGTYTLQCNISYNCYYDTGY
                     LEVTVVDPGESVIIEESFCEGENVTIDVTQEGSTYEWFDGSIESTYLFDESGTYWVTV
                     SLGECNFTDTIKITENANPTLELGSAITICGATEITAPSGYDSYSWSNGFTTPQFTTT
                     EAGTYTLTVTDENGCSTSDELIVINGCGDHIDFPTAFSPNGDHFNDYFEAIYSGDIKN
                     YHIEIFNRWGELVFETNKLEKGWDGKYENELQPMGVFTYRVTAALNEKNLVKQGNFVL
                     VR"
BASE COUNT          435 a          266 c          284 g          450 t
ORIGIN      
        1 aatattattg aaggtgaatg atagcaatat ggttcacctt tttttatgct tcgcgtttgc
       61 gtttgtaaat ttttacggca atcatcataa ttactgccag caacaataat ccgagaagac
      121 catacaacat gtttgccgta ttttttaatt cgattagaaa tacgatggca accagaaaaa
      181 tagtggctaa ctcgttaaat aatcgcaatt gttgtgagct gagttttgat gttttattgc
      241 ttaagcgttt ttgaatgcgc aaacaactaa aatggtaggt aatgacgcat agaataaaaa
      301 ataatttcat gtgcatccag ccttcttgca aaaactgctg attaaaatac aataaactac
      361 ttccggttat tactaccaaa atcatggaag gtgtgccaat aatagacatg aggcgacgtt
      421 ccataatggt gaattgtgtt gcgagtatcg atttgtcggg ttcgttgcgc tgcaaggctt
      481 cggtgtgata aatatataag cgcacaatat aaaataacgc tgcaaaccag gttacaataa
      541 aaataatatg tgctgcaaca aaataactgt aataagccat gcttttggat tgagtaatta
      601 ccttacgagt acaaaattac cttgtttcac taaatttttt tcatttaatg cggcagttac
      661 tctgtaggta aatacgccca ttggttgtaa ttcattttca tatttgccat cccaaccttt
      721 ttctaattta ttggtctcga aaacaagttc tccccaacga ttaaaaattt cgatatgata
      781 attttttatg tcgccggaat aaatggcttc gaaataatcg ttaaagtggt cgccattcgg
      841 agaaaatgca gttggaaaat cgatatgatc gccgcatccg ttaattacta ttaattcgtc
      901 ggaagtggaa cagccatttt cgtcggtgac agtcagggta taggtgccgg cttctgttgt
      961 tgtaaactga ggtgttgtaa atccattact ccaggaatac gagtcgtaac ctgaaggggc
     1021 agtaatttct gttgcaccac aaatggtaat tgcactgccg agttctaatg ttggatttgc
     1081 attttcggta attttaatgg tatcggtaaa attacattcg cctaaactaa cggtaaccca
     1141 ataggtgccg ctttcatcaa aaaggtaagt ggattcgatg ctaccatcaa accattcgta
     1201 tgtagaacct tcctgtgtta catcaatagt cacattttcg ccttcgcaaa atgattcttc
     1261 aataattaca gattcaccgg ggtcaacaac ggtaacttcc agatatccgg tatcgtaata
     1321 acagttatag ctgatattac attgcagggt ataagtgccg ggtccgggcc agtcggtaac
     1381 ggtcttggtt ctaaggtcgt cgccggtgtc aacaatacct gcggctaatt cccac
//
LOCUS       DAORKW010000743         1434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6638_length_1434_cov_3.103698, whole genome shotgun sequence.
ACCESSION   DAORKW010000743 DAORKW010000000
VERSION     DAORKW010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6638_length_1434_cov_3.103698"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLB46_17495"
     CDS             <1..207
                     /locus_tag="PLB46_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78379.1"
                     /translation="NKSYALLPDGAEEEIKGAVDLNLSANYAYSKYFNIFVNVNNMAS
                     FKYQRYYNYPSYGIQALGGLSFTF"
     gene            278..1294
                     /locus_tag="PLB46_17500"
     CDS             278..1294
                     /locus_tag="PLB46_17500"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRF78380.1"
                     /translation="MHQIAQHISQLLYDHNCVIVPGFGGFVTNYQPAKVHPTSFIFNS
                     PSKSVAFNVKLTNNDGLLVNQIAKSEGISHYQAERQINEFVAQIQSALQERKPVKLAG
                     IGRLIMDVENNIQFLADNSHNFLMQSYGLYSFTAQPIARDYVDSAVVKQMEAIVVEKP
                     VKKKKSTDALLPIAAVLLLTMLTMQIFIQSSMNGFNYSEIFGFQKVFTKNNYLLEKYE
                     PVKFEMNPSILFFRKTDTATLVTLETDLNTITTPETVNNTTIATPVASTYFISAGSFY
                     SDAEVEIVLAKLLAKGYTGYIKTWGKYKVAAVDIPEQTTPSAFRAAFVEATGIADAWV
                     ARNK"
BASE COUNT          453 a          269 c          264 g          448 t
ORIGIN      
        1 aataagtctt atgcgttgtt acctgatggt gcagaagagg aaattaaagg cgctgttgac
       61 ttaaacctgt cagccaacta tgcttattcc aaatatttca acattttcgt taacgtgaac
      121 aatatggcct cgtttaaata ccagcgctat tataactatc cttcctacgg aattcaggct
      181 ttgggcggat tgtcgttcac gttttaaaca atcggtggat ttcttcacat ttttttaaca
      241 cccggtccgt atgatatttg ccatataata cggatcggtg caccagatag ctcaacatat
      301 cagccaacta ctttacgatc acaattgtgt gattgtgccc ggttttggcg ggtttgtgac
      361 caattatcag cctgcaaagg tgcatcctac ctcctttatt tttaacagcc cttcaaaaag
      421 tgtggctttt aacgtgaaat tgaccaataa cgatggttta ttggttaatc agattgctaa
      481 aagtgaagga atcagccatt atcaggcaga aagacaaatc aatgaatttg ttgcacaaat
      541 acaatcagca ttacaagagc gtaaaccggt gaaattagcc ggcattggtc gtctgataat
      601 ggacgttgaa aacaatatcc agtttttagc cgataattct cacaatttcc tcatgcagtc
      661 gtacggtttg tattcattta ctgctcaacc tattgcgcga gattatgtag actcagctgt
      721 agtaaaacaa atggaggcta ttgttgttga aaaaccggtt aaaaagaaaa aatctactga
      781 tgcgttatta ccaattgcgg cagtattatt attaacgatg cttaccatgc agatttttat
      841 tcaatcgagc atgaacggtt ttaattattc tgaaattttt gggtttcaaa aagtgtttac
      901 gaaaaataat tacctcttag aaaaatacga acccgtaaaa ttcgagatga atccttctat
      961 tttatttttc agaaaaacgg atacggccac attggttact ttagaaactg atttaaacac
     1021 tattacaaca cctgaaacag ttaataacac aacaatcgca acccctgttg catcaaccta
     1081 ttttatatca gctggttcat tttattctga tgctgaagtg gaaatagtgt tggctaaatt
     1141 gctagccaaa ggttacaccg gttatataaa aacctggggt aaatataaag ttgctgcggt
     1201 tgatataccg gaacaaacta ctccttctgc atttagagca gcatttgttg aagctaccgg
     1261 cattgctgat gcttgggtgg cccgcaataa ataacgctaa atacatcttc acaaaaacag
     1321 aaaagctctt gtcagggctg gattaagtgt ttaaaacaac attcaatttt gttgctgatt
     1381 taacaaactg atattctcat ttaaatccaa tatacaccag tgttgtaaag ggat
//
LOCUS       DAORKW010000744         1428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6687_length_1428_cov_4.890750, whole genome shotgun sequence.
ACCESSION   DAORKW010000744 DAORKW010000000
VERSION     DAORKW010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6687_length_1428_cov_4.890750"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..>1428
                     /gene="porV"
                     /locus_tag="PLB46_17505"
     CDS             157..>1428
                     /gene="porV"
                     /locus_tag="PLB46_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HRF78381.1"
                     /translation="MQTLDFRRLYMRNFMKFTWMVVAVIAVSTSSKAQLSIGNTGDEY
                     LNTVTTAVPFLRITPDARSGGMADVGIAITPDANSIFWNASKIVFIDDAHPTGISLTY
                     TPWLKNLVNDIYLAYLNGYWKVDELSAVSASLRYFSLGNITFTDASGNVLQDFRPNEF
                     AFDFAYSRKLADNLSAGLDLKYIYSNLATGQVVNGVEIKPARGVAADFSMFYTNQFDT
                     GDKDSEIGIGLNISNIGNKVTYTSSIEKDFIPTNLGIGAAYGIHFDSYNKLTFALDLN
                     KLLVPTPDSADSNPQNGILDFKEQSMVSGMLSSFGDAPNGISEELHEIIYSAGVEYWY
                     DEKFAVRSGYFYEHATKGGRQFFTVGLGLRYNVFGLDFSYLVPTSSNQNPLDNTLRFT
                     LQFNLDALGTASGLGECGVRRGGRRLGLGLSL"
BASE COUNT          407 a          269 c          287 g          465 t
ORIGIN      
        1 gggacggctt agatgaatac ggcgatgcta taggtaaggg tgtttatgtt tacaagctca
       61 ccgtgaaggc tgccagcgac aattcaaagg caaatgaatt tcaaaaactg gttatactaa
      121 aatgatgttg ctaaacgtta tagataattg ttatttttgc aaaccctaga ctttagaaga
      181 ttatatatgc ggaattttat gaaatttacc tggatggttg ttgcggtaat tgcagtttca
      241 acctcatcaa aagcacagct ctccatcggc aataccggtg acgaatacct taacacggtt
      301 actacagcag ttcctttctt acgcattact cctgacgcac gttcaggcgg tatggctgat
      361 gtgggtattg caattacacc tgatgctaac tctatttttt ggaatgcctc taaaattgtt
      421 tttattgatg atgcacatcc aacaggcata tcacttactt ataccccatg gcttaaaaat
      481 ctggtaaatg atatttacct ggcttactta aacggatatt ggaaagtaga cgaattgtca
      541 gcagtaagtg cttctcttcg ttatttctca ttaggtaata tcacatttac cgatgcttcc
      601 ggaaacgttt tacaagattt tcgtcctaat gaatttgcat tcgattttgc ttattcacgt
      661 aaattagccg ataacctttc tgcaggtctc gatttaaaat atatttattc taaccttgca
      721 accggtcagg ttgtaaatgg tgtagaaatt aaacctgctc gtggtgttgc tgcggacttt
      781 tcgatgtttt ataccaatca attcgataca ggtgataaag attctgaaat tggtatcggt
      841 ttaaatattt ctaatatcgg aaataaagta acttacactt ccagtatcga aaaagatttt
      901 attccaacta accttggtat tggtgctgcc tatggcatcc acttcgatag ttataataaa
      961 cttactttcg ctttagactt aaataaatta ttagttccaa cacctgatag cgcagacagc
     1021 aacccacaaa acggtattct tgacttcaaa gaacaaagta tggtaagtgg tatgttgagt
     1081 tcatttggtg atgcaccaaa cggtatcagc gaagaattac acgaaattat ttattctgcc
     1141 ggtgttgaat attggtatga tgaaaaattt gctgtgcgtt caggatattt ttatgagcat
     1201 gcaacaaaag gtggccgtca attctttact gttggtttag gattacgtta caatgtattt
     1261 ggattagatt tctcttattt agtacctaca agcagcaatc aaaacccttt ggataacact
     1321 ttgcgtttta cgttacaatt taaccttgat gctttaggca ccgcgtcggg cctcggcgag
     1381 tgcggcgtcc gacgcggtgg gcgcaggctc gggctcgggc tcagcctc
//
LOCUS       DAORKW010000745         1422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6747_length_1422_cov_4.367228, whole genome shotgun sequence.
ACCESSION   DAORKW010000745 DAORKW010000000
VERSION     DAORKW010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6747_length_1422_cov_4.367228"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PLB46_17510"
     CDS             <1..1009
                     /locus_tag="PLB46_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78382.1"
                     /translation="GWSATISNVACAGGPTGTDNSDCNYATPICSDVAVAASDVGPGL
                     VSESCSGCTAGGENYSTWYTFTMATGGNIGLTIDPVTNSDDYDFALYGPGVTCGGLGT
                     PIRCSYAATTGDTGMKTTALDVSETVTGNGWVATLDVLAGETYYLMVNHWSPPVTGYT
                     LDWNLNLGATFDCALLPLTLTSFNAELIENNSVALGWETSTEFNNDYFLVERSTDGVN
                     YSSIGTVKGKQYSEEPTNYYMVDFLPYSGTNYYRLSQVDLNGISNILKTTSVNVDLNT
                     GYGVIEVYDMSGHLIVKELIYRHQIDLLMSSLQLVPGIYTYNYVQQNGYRQTDKLAIT
                     R"
     gene            complement(1108..>1422)
                     /locus_tag="PLB46_17515"
     CDS             complement(1108..>1422)
                     /locus_tag="PLB46_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRF78383.1"
                     /translation="ILPAEWYVFAKINCIPNMLQIDNIVLSHDQLPEITNGVINFFGH
                     LHPAVRLSGAAKQSLRLSCFWLGENFMVMPAFGRFTGAKSIRPEKTDTIYAVGEGEIF
                     KL"
BASE COUNT          445 a          238 c          277 g          462 t
ORIGIN      
        1 aggttggtcc gctacaatca gcaatgtggc ttgtgccggc ggacctacag gtacagataa
       61 tagtgattgc aattatgcaa ctccaatttg ttcagatgtt gccgtagctg caagtgatgt
      121 tgggccggga ttagtttcag aatcatgttc aggatgcaca gcaggtggtg aaaattattc
      181 gacttggtac acatttacga tggctacagg aggcaatatt ggtttgacaa ttgaccctgt
      241 tacaaatagt gatgattatg atttcgcttt atatggacca ggcgttacat gtggtgggct
      301 tggcacacca atacgatgca gttatgctgc aacaacaggt gataccggta tgaaaacaac
      361 tgctctggat gtatcagaaa cagttacagg gaatggttgg gtggcaacat tagatgtact
      421 tgcgggagaa acatattatt taatggtaaa tcactggtca ccaccggtta ctggatatac
      481 attagattgg aatttaaatc ttggtgcaac attcgattgt gctttattgc cattaacact
      541 tacatcattt aatgctgaat taatagaaaa taatagtgtt gctttaggat gggaaacttc
      601 aactgaattt aataatgatt attttttagt tgagcgttct acagatggtg ttaattattc
      661 ttcaattgga acagtaaaag gcaaacaata cagtgaagaa ccaactaatt attatatggt
      721 agatttctta ccttattcag gtactaacta ctatcgtctt tctcaagttg atttaaacgg
      781 cataagcaat atattaaaaa ccacatctgt aaatgtagat ttaaatacag gatatggagt
      841 tattgaagtg tatgatatgt cggggcattt aattgttaaa gaattaattt accgccatca
      901 aattgattta cttatgtcat cgctgcaatt agttccgggt atttatacct ataattatgt
      961 tcagcaaaat gggtatagac aaactgataa actcgctatt acccgttaat gattttcgtt
     1021 ttcttttaaa ttcggttact actgattaat tggtagcata tttccattac ttatattttg
     1081 agttgctaac ccaaatggaa accaatttta tagtttaaat atttctcctt ccccaacggc
     1141 ataaatggta tccgtttttt ccgggcgaat actttttgct ccggtaaaac gcccaaatgc
     1201 cggcattacc ataaaatttt ctcccaacca aaaacaggaa agtcgcagcg attgttttgc
     1261 agcaccactt aatcttactg caggatgtag atgaccgaaa aaattgataa caccatttgt
     1321 aatttccggt aattgatcat gagacaatac aatgttgtca atttgcaaca tgtttggaat
     1381 acagttaatt tttgcaaaaa cataccattc ggcaggtaaa at
//
LOCUS       DAORKW010000746         1415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6808_length_1415_cov_3.919853, whole genome shotgun sequence.
ACCESSION   DAORKW010000746 DAORKW010000000
VERSION     DAORKW010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6808_length_1415_cov_3.919853"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PLB46_17520"
     CDS             complement(<1..1121)
                     /locus_tag="PLB46_17520"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRF78384.1"
                     /translation="MQTFIDFISLNDPNVLFVVIGMILLGISSATIGTFAFLQKRALT
                     GDAIAHSVLPGVCLAFILFDTRNLWVLLGGAFVTGWLSIVCVDIITKHSKLKADTAIG
                     LVLSVFFGVGILLLTFIQQSGNAAQSGLNNFLFGKAAAMHPDDIKTMGIVSLLIIIIV
                     LTFFKEFKLLSFDPAYAKSTGLPVTVLQLLLTTLTVLAVAAGIQAVGVVLMAAMLITP
                     AAAAKYWTNNLRKMILLSILFAVAGSVIGAYVSYTDNKMPTGPWVVMMISCIAVLSIL
                     FAPKKGIIPKYIIRKKYVNKMLRENILKEMYHLEEKNPGKKIFSIHDITSERAMKSSE
                     YKLGFRMLTRKHLVNKVVTGYGLTNEGQIEGKRITRIHR"
     gene            complement(1125..>1415)
                     /locus_tag="PLB46_17525"
     CDS             complement(1125..>1415)
                     /locus_tag="PLB46_17525"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRF78385.1"
                     /translation="DLFDFWSIGLNTGADWNEEENYLQFPFSVSNAFSLGDKMGYFVE
                     VFGPDFTENGFSLDAGLTYLISDNFQVDISGGFNFGYETSFISTGFAYRFEL"
BASE COUNT          488 a          293 c          217 g          417 t
ORIGIN      
        1 aaacggtgaa tacgagtaat acgttttcct tctatttgtc cttcgttggt aagcccataa
       61 ccagtcacta ctttatttac caaatgttta cgcgtcaaca tccgaaaacc tagtttgtat
      121 tcgctgcttt tcatagcgcg ctcagaagta atatcatgaa tggaaaatat ctttttcccc
      181 ggattttttt cttcaaggtg atacatttct tttaaaatat tttcccgcaa cattttgttg
      241 acatactttt tacggataat atattttgga ataattcctt ttttaggggc aaataaaata
      301 cttaatacgg caatacatga aatcatcatc accacccagg gaccagtagg cattttattg
      361 tcggtataag aaacataagc accaattaca ctgcctgcca ctgcaaataa aatgcttaat
      421 aaaatcattt ttcgcaggtt atttgtccag tactttgctg ccgctgccgg tgtaatcagc
      481 atagcagcca ttaataccac accaactgcc tgaatacccg cagcaactgc taatacagtg
      541 agtgtagtta ataataattg taatacggta accggtaatc ccgtgctttt agcatatgca
      601 ggatcaaaac ttaataattt aaattctttg aaaaaggtta gcacaataat aattatgagt
      661 aaactcacaa tccccattgt tttaatatca tcaggatgca tggccgcagc tttgccaaat
      721 aaaaaattgt ttaaaccact ttgagctgca ttaccactct gctgaataaa ggtgagcagt
      781 aaaatcccaa caccaaaaaa taccgatagt actaatccga ttgctgtatc tgcttttaac
      841 ttactatgtt tggtaataat atctacacaa acaatagaaa gccatcctgt aacaaatgca
      901 ccgccaagta aaacccataa atttctggta tcaaataaaa taaacgctaa acaaacaccc
      961 ggtaaaacgg aatgcgctat ggcatcgcct gttaatgcgc gtttttgcaa aaatgcaaac
     1021 gtacctatag tagccgaact tatcccaagc agtatcatgc cgattaccac aaataaaaca
     1081 ttggggtcgt tgagcgatat aaagtctata aatgtttgca tatattataa ttcaaaccga
     1141 taagcaaatc ctgtactgat aaaacttgtt tcgtaaccaa agttaaatcc accggaaata
     1201 tctacttgaa aattgtctga tatcaagtag gtaagtcccg catcaagaga aaaaccattt
     1261 tctgtaaaat cagggccaaa cacttctaca aaataaccca ttttatcgcc taatgaaaat
     1321 gcattggaaa cagaaaatgg aaattgtaaa taattttctt cctcattcca gtccgctccc
     1381 gtatttaatc cgatggacca aaaatcaaag aggtc
//
LOCUS       DAORKW010000747         1403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6942_length_1403_cov_5.897626, whole genome shotgun sequence.
ACCESSION   DAORKW010000747 DAORKW010000000
VERSION     DAORKW010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6942_length_1403_cov_5.897626"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..707)
                     /locus_tag="PLB46_17530"
     CDS             complement(216..707)
                     /locus_tag="PLB46_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78386.1"
                     /translation="MNTSAIIFGIIVILIIVLPIVMLNTYRKKREKRLFQQLAQFAGN
                     QHATVTLYEVWGTSAIGMDEAQNKLFFTKNIQGMEIFMIATLPEIEKCTINTITRMVR
                     DNESNISVTDKLELVLRFIDKSKEDLILPIYNAKTDSLTLSGELQLIEKWNTIINKSL
                     KSK"
     gene            complement(929..1306)
                     /locus_tag="PLB46_17535"
     CDS             complement(929..1306)
                     /locus_tag="PLB46_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78387.1"
                     /translation="MNSKINKRQLFPVVVAYCLLLFCYAFQICYFQNQTFNSDSFPED
                     QYQHQGFAEAIGAVANTERLQIAGKFNSITPFKTIIESVQKIEAENKGYTCSLFNQYT
                     FYTNYLILRLLQSAIIFPFHYFW"
BASE COUNT          444 a          205 c          264 g          490 t
ORIGIN      
        1 aattattata atccaaaatt agcgacagga atttatttgg ttgtattaac agataataaa
       61 ggcattcgat ttgtggaaaa ggtgttgttg agagattgat ggggtgcacc cgcctcagcg
      121 gactaaaagc gcggagaggg gagttggctg agactttata aaaaaattat ggacattacc
      181 ttttccggta atgtccacat aaaaattgat tataattact ttgattttaa cgatttattg
      241 ataatagtat tccacttttc aattaactgc agttcaccgg ataaggtcag actatcggtt
      301 tttgcattat aaatgggtaa aataaggtct tctttggact tgtcaataaa tcgcaaaact
      361 aattctagtt tatctgttac actgatatta gattcattgt ctcttaccat tctggtgatg
      421 gtatttatcg tgcatttttc tatttcgggt aatgttgcaa tcataaatat ttccataccc
      481 tgaatatttt tggtgaaaaa taatttgttt tgcgcttcat ccatgcctat ggctgatgtt
      541 ccccaaactt cgtaaagggt tacagttgca tgctggttgc ctgcaaattg agctaattgt
      601 tgaaataacc ttttttcgcg ttttttgcgg taggtattta acattacaat aggtaaaacg
      661 attattagaa tcacaatgat tccaaatatg atagcactgg tattcataac tttattttga
      721 aagtttgttc agatataaaa atatctgact gattaattta tttgttcatt aggaaatact
      781 ggtatgtata caatatgtat ataaagtaac agaccacgca ttacattaat taatttgtaa
      841 ttgctacatt aacaagctac ttaaaacttg aaaatctagg tacccatttg cagggtcaaa
      901 tacacgtaat gagtggtatt aaatatgatt accaaaaata atgaaaaggg aagatgatag
      961 ctgattgaag taaccgtagt attaaatagt tggtataaaa tgtatactga ttaaataagc
     1021 tgcaagtata tcctttgttt tcagcttcta ttttttgtac ggattctatt attgttttaa
     1081 aaggtgtaat tgaattgaat tttcccgcga tttgaagcct ttcagtatta gcaactgcac
     1141 ctattgcttc ggcaaaaccc tgatgttggt attggtcttc ggggaaacta tcggaattaa
     1201 atgtctgatt ttggaaataa catatttgaa aggcgtaaca aaacagcaac aggcagtagg
     1261 ctactaccac cgggaataat tggcgtttat taattttgct gttcaccgca caaaggtaat
     1321 aagaaaatgt tattgggggg ccagcaatta ctgccttttg attaatatac cggtttcgtt
     1381 tcgccagcta ttggataaat ggt
//
LOCUS       DAORKW010000748         1403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_6946_length_1403_cov_3.808605, whole genome shotgun sequence.
ACCESSION   DAORKW010000748 DAORKW010000000
VERSION     DAORKW010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6946_length_1403_cov_3.808605"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PLB46_17540"
     CDS             complement(<1..1172)
                     /locus_tag="PLB46_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78388.1"
                     /translation="MKKIYFSSLLAIALLSVMQNPAAAQLKEGDVAPDWTLTDINGTE
                     WNLYTLLNEGKSVFLDFSAVWCGPCWGYHTGGNLETLYETYGPDGTNEVMVFYIEGDG
                     GSTIDQLNGIGGGTQGDWVDGTPYPIILTHAGDDSYDVVQDYDIGYFPTIYRVCPNRI
                     IKEVGQAAVTTLYNSINSCEIAFTNNDPAIIAYTGPTAACGEVDLSVNIQNIGFDNLT
                     ACTFGVYDGEGIEILTYEWTGDLDLYEFAVVNIGTVTLTGDDNDLEIKIISTDENDDN
                     NAVDANITYKNNVSMIVHLKVKTDNYPTQTRWNIVDDETGEELYEDGPYSTSMKNEIV
                     FDEDLILPGTGCYTFTFFDNAGDGLTGSGYFELTDAADIMLAEGDEDVAFKKMASL"
BASE COUNT          414 a          295 c          243 g          451 t
ORIGIN      
        1 tttaatgaag ccattttttt gaatgcaaca tcttcatcac cttctgccaa cataatatct
       61 gcagcatcgg ttaattcaaa atatccggat ccagttaagc catctccggc gttatcgaaa
      121 aatgtaaatg tataacaacc tgttccaggt aaaataagat cttcatcaaa tacgatttca
      181 tttttcatac tggtagaata aggaccatct tcatacagtt cttcaccagt ttcgtcgtct
      241 acaatattcc aacgtgtttg tgttgggtaa ttgtctgttt ttactttaag atgaacaatc
      301 attgatacat tgtttttata tgtaatattt gcatcaactg cattgttatc atcattttcg
      361 tcggtagaaa taattttaat ttccaagtca ttatcgtcac ctgttaacgt aacggtacca
      421 atatttacca ctgcaaattc atataaatct aaatcacctg tccactcata ggttaaaatt
      481 tcgatgcctt caccatcata aacgccgaaa gtgcaggccg ttaaattatc gaaaccaatg
      541 ttttgaatat ttacagataa atcaacttca ccgcaggctg ctgttggtcc ggtataagct
      601 ataatggcag ggtcgttatt ggtaaatgca atttcgcagc tatttatact attgtataaa
      661 gtagttacag cagcttgtcc cacttcttta atgattctat taggacaaac tcgataaata
      721 gtagggaagt agccgatatc ataatcttga acaacatcat aactatcgtc acctgcatgt
      781 gttaaaatga ttggatacgg tgtgccatct acccagtcgc cttgagtgcc accacctatt
      841 ccgttcaatt ggtcaatagt gctaccacca tcaccttcaa tataaaacac cattacttca
      901 ttggtgccat ccggaccata agtttcatat aatgtttcca agttaccacc tgtatgataa
      961 ccccaacacg gaccgcacca aacagctgaa aaatcgagga aaaccgattt gccttcattc
     1021 aaaagcgtat acagattcca ctcagttccg ttaatatcgg tgagagtcca gtcaggagcc
     1081 acatctccct cttttaattg tgcagctgcc gggttttgca tcacgcttag tagtgcaatg
     1141 gcgagcagac ttgaaaagta gatttttttc atcgttttaa aaattgtttt caaagctaat
     1201 catttcctga tatttatagt agggttttcc actagggttt tgtgggcttg gtcttagtgt
     1261 cctgagataa ggtaaatatt ccgcaatttt tgtgaacagg aaaattggct gctttgcccg
     1321 ccctgcaagc gttgtaggcg atgactaagc ggtaatttta gtttcagcca acaaggtcaa
     1381 aaccgtgcat ttgtctgaca aac
//
LOCUS       DAORKW010000749         1393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_7054_length_1393_cov_3.513453, whole genome shotgun sequence.
ACCESSION   DAORKW010000749 DAORKW010000000
VERSION     DAORKW010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7054_length_1393_cov_3.513453"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="PLB46_17545"
     CDS             complement(<1..1353)
                     /locus_tag="PLB46_17545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRF78389.1"
                     /translation="MFLYKFMNDKFGHEVKQLEPKLAKSENWEKEIAAYTDKQYEMLL
                     LRMNPDSAKLKRMHFLSNLYKQQDKADFALLFDDTLRDIAIFNNDIFSVKTGTGAKIV
                     LFDELSNYITDKSQRDAFCKAIINQLVDFSFEKIFNQKFDFFSTIFEYLINDYNKDGG
                     GKYAEYYTPHAVSKIMAAILVDKPVSNVTCYDPSAGSGTLLMNLAHAIGEDRCTIYSQ
                     DISQKSSSMLRLNLILNNLVHSIQNIIQGNTIKSPFHKVGDKLMTFDYIVSNPPFKLD
                     FSDYIADLDTKQNTDRFFAGLPNVPKKDKDKMAIYPLFIQHIMFSLKEGKGKAAIVVP
                     TGFITAQSGIEKKIRERLVEEKMLRGVVSMPSNIFATTGTNVSILFLDKANTKGDIVL
                     MDASKLGATVKEGKNQKTVLSDEEELLIVATFNKHEVKEGFTVVVSYEQIKEKNYSFS
                     A"
BASE COUNT          429 a          258 c          221 g          485 t
ORIGIN      
        1 agcgctaaag ctgtaatttt tttctttaat ttgttcatat gacaccacaa ccgtaaaacc
       61 ttctttaact tcatgcttat taaacgttgc aacaataaga agctcttcct catcagaaag
      121 aactgttttt tggttttttc cttcttttac agtcgctcca agtttagagg catccatcaa
      181 tacaatgtcg cctttagtat ttgctttatc taaaaataaa atagatacgt tagtgcctgt
      241 tgttgcaaaa atattacttg gcatgctcac gacacctctg agcatttttt cttctaccaa
      301 acgttcacga attttctttt caattccgct ttgcgcagta ataaatcctg taggtacaac
      361 aatagcagct ttaccttttc cttcctttaa tgaaaacata atatgctgta taaacaatgg
      421 gtaaatcgcc attttgtcct tatccttttt agggacatta ggcaatcctg caaaaaaacg
      481 gtcggtgttt tgtttggtgt ccaaatcagc tatataatca ctaaagtcta acttaaaggg
      541 tgggtttgaa acaatatagt caaatgtcat taatttatca ccaaccttgt ggaaaggaga
      601 tttgattgta ttaccctgaa tgatgttttg aattgaatgc actaaattgt tcaatatcaa
      661 attcaaccgc agcatactgg atgacttctg cgaaatatct tgagagtaaa tggtacatcg
      721 gtcttcacca atggcatgtg ccagattcat aagtaaggtg cccgaaccgg cagatggatc
      781 gtaacaagtc acattactca cgggtttgtc aaccagaatt gcagccataa ttttcgaaac
      841 tgcatgcggg gtgtagtatt cagcatattt acctccacca tctttattat aatcgtttat
      901 aagatattca aatatggttg aaaagaaatc aaatttctga ttaaagattt tctcgaaact
      961 gaaatccacc aattggttaa tgatagcttt acagaaggca tcacgctggc ttttgtcggt
     1021 gatgtaatta ctaagttcat caaataatac aattttggca cctgttccag ttttcacaga
     1081 aaaaatatca ttattgaaaa tagcaatatc tctcaatgta tcatcaaata gcaaagcaaa
     1141 atctgctttg tcttgttgct tgtataaatt agatagaaaa tgcattcttt ttagcttagc
     1201 gctatcagga ttcatacgta ataataacat ttcgtattgc ttgtcagtgt atgcagctat
     1261 ttccttttcc cagttttctg attttgcaag tttaggttct agttgtttta cctcatgacc
     1321 gaatttgtca ttcataaatt tataaagaaa cacctgggta ataattttaa attcattacc
     1381 atcgttacct aat
//
LOCUS       DAORKW010000750         1378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_7233_length_1378_cov_2.372638, whole genome shotgun sequence.
ACCESSION   DAORKW010000750 DAORKW010000000
VERSION     DAORKW010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7233_length_1378_cov_2.372638"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..>1378
                     /locus_tag="PLB46_17550"
     CDS             63..>1378
                     /locus_tag="PLB46_17550"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78390.1"
                     /translation="MLSYITKIFTLITVYFIATIAVYAGPGDTIKVQTFTFGSPQDAW
                     FELPGLDVDVEKILMKYTLKCNPAQSPACGEWDYLTYTYLYEHTGNLDSTLLTHPYFT
                     ANGASPDELNYSSTPTYTNVPSWQYSIVYDDTITYNSYQSGVADSYAFYPFYTAYPVG
                     RTQILWRADELTAAGLTPGNITGMDFEINSPGDEMKHLSIRMKSSVLTEFPSNVFESG
                     FTEVYNQNTTFDLGWDGVDFTTPFYWDGLSNIIIEITYDNTTATGLSYMTSMTTTPYF
                     SLLTANTNDKYVEFLTPDYADVIGNPLSGLSDQVTVAFWSQGNADFQPQNGTTFEAVT
                     SSGARVLNSHTPWSDSNVYWDAGDDGGYDRINKVAVAADYEANWTHWAFTKNTTTGSM
                     KIYKNGVLWHSGTGKTKLMEGIDKLRLGKGNWGGSESYAGNMDEFA"
BASE COUNT          422 a          261 c          277 g          418 t
ORIGIN      
        1 atccccttgc ctcaggtttg ttaaaaccta tacttggcta tatttgtgtc aaacttacaa
       61 ccatgttgtc atacattact aaaattttta cactcattac tgtttatttt atcgcaacaa
      121 tagcggtata cgcgggtccc ggcgatacca ttaaagtgca aacctttacg tttggctcac
      181 ctcaggacgc ttggtttgag ctgccgggtt tagatgtgga cgtggaaaaa atcctgatga
      241 aatacaccct caaatgtaat cccgctcaaa gtccggcttg cggcgaatgg gattatttga
      301 cgtataccta tttgtatgaa cataccggaa atttggattc aaccttgtta acccatccat
      361 attttactgc caatggtgcc agtcccgatg aattgaatta ttcctcaaca ccaacttaca
      421 ccaatgtgcc ttcatggcaa tattcaattg tttacgacga taccattacc tataacagtt
      481 atcaatcagg tgttgccgat agttatgcat tttatccttt ttataccgca tatccggttg
      541 gcagaacaca aattttatgg cgtgctgatg aattaactgc tgcaggttta actcctggca
      601 atatcaccgg tatggatttc gaaataaata gcccgggtga tgaaatgaaa catttaagta
      661 ttcgcatgaa atcttcagtg ctgacagaat ttccatctaa cgtttttgag tcgggattta
      721 ctgaagtgta taatcaaaat accacattcg atttaggttg ggatggtgta gattttacaa
      781 caccgtttta ttgggatggt ttatccaata ttattattga aattacttat gataatacca
      841 ctgcaacagg attatcgtac atgacaagta tgactactac accatatttt tcattgctta
      901 ctgcaaatac caatgataaa tatgttgaat ttttgactcc tgattatgca gatgtaattg
      961 gcaatccttt atccggttta agcgaccagg taacagttgc attttggtct cagggaaatg
     1021 cagattttca accacaaaac ggaacaacat tcgaagctgt tacaagcagc ggagcaagag
     1081 tattaaatag tcacacccca tggagcgata gtaatgtata ttgggatgcc ggtgatgacg
     1141 gtggttacga tcgtattaat aaagtagcag tagctgcaga ttacgaggcc aactggaccc
     1201 attgggcatt taccaaaaat accacaacag gttcaatgaa aatatataaa aatggtgtgt
     1261 tatggcattc cggtacaggc aaaacaaaat tgatggaagg catcgataaa ttgcgtttag
     1321 gaaaaggcaa ttggggtggc agcgaaagtt atgcaggaaa tatggatgaa tttgccat
//
LOCUS       DAORKW010000751         1371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_7285_length_1371_cov_4.760638, whole genome shotgun sequence.
ACCESSION   DAORKW010000751 DAORKW010000000
VERSION     DAORKW010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7285_length_1371_cov_4.760638"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PLB46_17555"
     CDS             complement(<1..680)
                     /locus_tag="PLB46_17555"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRF78391.1"
                     /translation="MNWSASIKEFKNYLQLEKSLSGNSVEAYLRDIGKLDSFLEKNYP
                     NTSPENVNREHLEGFLIHLHEINMNDRSQARIISGVRAFYKFLLMEDLIDNDPSQLLD
                     LPKLRRKLPDTLSYDEISSIIAAIDLSTPEGQRNKAIFETLYSCGLRVSELTCLRISD
                     MFMDDGFVKVKGKGNKERLVPIGDSAINYIKIYKELVRSHIAVQKGFEDHLFLNRNGR
                     ALSRVYIF"
     gene            764..1183
                     /gene="aroQ"
                     /locus_tag="PLB46_17560"
     CDS             764..1183
                     /gene="aroQ"
                     /locus_tag="PLB46_17560"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRF78392.1"
                     /translation="MKIIIINGPNLNLLGLREPEIYGSETFENYFQKLQLRFPNVELA
                     YFQSNVEGILIDKLHEVGFSYDGIIINAGGYSHTSIAIADAISSIKTPVVEVHISNTF
                     AREAFRHNDVLTAKCKGLIVGLGLEGYALAINYFLKH"
BASE COUNT          379 a          252 c          290 g          450 t
ORIGIN      
        1 atgaagatat aaaccctgga aagtgctctg ccattgcgat ttaaaaacag gtggtcctca
       61 aagccttttt gaaccgctat atgacttctc accaattcct tataaatctt gatataattt
      121 atcgcactat cccctattgg caccaaacgt tctttgttgc ctttgccttt tactttcaca
      181 aaaccatcat ccataaacat atcgctaata cggagacagg tcagctcact gacacgcagt
      241 ccgcaactgt ataaggtctc aaaaatggct ttattacgtt gcccttccgg tgtactgagg
      301 tcaatagcgg ctataatact gcttatttcg tcataagaaa gggtatcagg caattttcta
      361 cgcagtttgg gcaagtccag cagctgactg ggatcgttgt ctataaggtc ttccatgagc
      421 aaaaacttgt aaaacgccct tacaccgctg ataatcctgg cctgggacct gtcgttcatg
      481 tttatttcgt ggaggtggat gagaaaacct tccaggtgtt cgcgatttac gttttcggga
      541 ctggtattcg ggtagttttt ttccaggaag ctatccagct tgccaatatc acgcaggtag
      601 gcttctaccg aattaccgga cagggacttt tcgagttgga ggtagttttt aaattccttg
      661 atggatgcgg accagttcac ggtgaaaaga tagatgcatt ttaatcaacg cttttaagta
      721 ttatgctatt accaattgtg aataatcgta aatttgtcca ctaatgaaaa tcatcatcat
      781 caacggacct aatctcaatt tattaggatt gcgcgagccg gaaatttacg gaagcgaaac
      841 ttttgaaaac tattttcaaa aattacaatt gcgtttccct aatgttgaat tggcctattt
      901 ccaaagtaat gttgaaggta ttttaattga taagttgcat gaagtgggat ttagttatga
      961 tggtattatt atcaacgctg gtggttattc ccatacttcc atcgctattg ctgatgctat
     1021 tagttcgatt aaaacgcctg tagttgaagt gcatatatca aatacttttg cccgcgaagc
     1081 ttttcgacat aatgatgtat tgacggcaaa atgtaagggt ttaattgttg gattgggttt
     1141 ggaagggtat gcacttgcga tcaattattt tttgaagcac tgattcggat gagggatgag
     1201 tgatgagtga tccgctggag cggacatgag tgatgaggga tgactgctaa atatttcgtg
     1261 tagtattact cgatttcaat tttgggataa tggtgttaga tgattttttt cttctaaaaa
     1321 tgttaattat tcttacctta tatagctttt taacagccga aaccacggag t
//
LOCUS       DAORKW010000752         1368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_7321_length_1368_cov_3.862909, whole genome shotgun sequence.
ACCESSION   DAORKW010000752 DAORKW010000000
VERSION     DAORKW010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7321_length_1368_cov_3.862909"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..>1368
                     /locus_tag="PLB46_17565"
     CDS             109..>1368
                     /locus_tag="PLB46_17565"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRF78393.1"
                     /translation="MRKIFTLITIFAMCASLQLGAQTRTVTGVVLDGGNNQPIPGASV
                     IAKEDPTTGKATDLDGKFSLSVGDNVTTLVISSLGFTTQEVVIGTQSNITVKMVTADY
                     NLDAIVVSASRRQEKILDAPASITLISPEQLKNTAAVTPMANLKGVPGVDIINTGLVQ
                     TNVVVRGFNNIFSGSLLTMVDNRYAAVPSLRANVATFIPTDNIDLERIEILRGPASAL
                     YGPNCSNGVCHMITKSPLAQEKPYMTTASIGLGFRSKMSDTILISNPIDPATGNIYAD
                     YTPLFDYDNIGDRMFYTASFRHSGKINDKFGYKILFTYLDGTDWLYDDPFEPAQIQLG
                     YQTAEGRVNVGDSIDNIRNHHIQKIGLDLRFDYKLKNSGELIFSGGMTENDGIELTGI
                     GAGQAINWKYYYAQARYLYKGWFAQAFI"
BASE COUNT          432 a          259 c          275 g          402 t
ORIGIN      
        1 actatttttg gtgatatata aacgattaaa caacaatgta ggttgtgacc aatttgatgt
       61 aaaaatagca accaaaccat taatccaact taaacaaacc ctcagctcat gagaaaaatt
      121 tttacactaa ttaccatttt tgcaatgtgt gcttccctcc aactgggggc tcaaacccgc
      181 acagttacag gagttgtgct cgatggaggt aataaccagc ctattccggg cgcctctgta
      241 attgccaaag aagacccaac cacgggcaaa gcaacagatt tagatggaaa gtttagcctc
      301 agtgttggcg ataatgtaac cacacttgtt attagcagcc ttggttttac tacccaggaa
      361 gtggtaatcg ggacccaaag taacattaca gttaaaatgg tgactgccga ttataacttg
      421 gatgcaattg tagtgagtgc ctctcgtcgt caggaaaaaa tattggatgc gccagcttct
      481 ataacattaa tttctcctga acagctgaaa aatactgccg cagttactcc aatggctaac
      541 cttaaaggtg ttccgggagt tgacattatt aatacaggat tagtgcaaac caacgtagtt
      601 gtgcgtgggt ttaacaatat cttttcgggt tcactattaa ctatggtgga taatagatat
      661 gctgcagtgc caagtttaag agccaacgta gccactttta tcccaaccga taatatcgac
      721 cttgagcgaa ttgaaatatt gcgtggtccg gcctctgcgc tttatggtcc aaactgttca
      781 aatggtgttt gtcatatgat aacaaaatcg ccgttggcac aagaaaaacc gtatatgacc
      841 accgcatcaa ttggtttagg tttcagaagt aaaatgagtg atacgatatt gattagtaat
      901 cctatagatc ctgcaaccgg taatatatat gctgattata cgccgctttt tgattatgac
      961 aatattggtg acaggatgtt ttatactgca tcattcagac attcaggaaa aattaacgac
     1021 aaattcggat ataaaatcct cttcacctat ttagatggaa cggattggtt atatgatgac
     1081 ccttttgagc ctgctcaaat tcaattaggt taccaaaccg cagaaggtcg cgttaatgtt
     1141 ggtgattcca ttgataatat tcgcaatcat catattcaaa aaattggtct tgatttacgg
     1201 ttcgattata aattgaaaaa ttcaggagaa ttaatattta gtggcggtat gaccgaaaac
     1261 gatggtatcg aattaaccgg tatcggcgca ggtcaggcta tcaactggaa atattattat
     1321 gcacaagcca gatatttata caaaggatgg tttgcacaag cttttatc
//
LOCUS       DAORKW010000753         1367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_7337_length_1367_cov_4.588415, whole genome shotgun sequence.
ACCESSION   DAORKW010000753 DAORKW010000000
VERSION     DAORKW010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7337_length_1367_cov_4.588415"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..>1367)
                     /locus_tag="PLB46_17570"
     CDS             complement(317..>1367)
                     /locus_tag="PLB46_17570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78394.1"
                     /translation="EIAYMFSGKNIYAKAMHINSASNIFIWGNYDKNPAGTNKDMFLA
                     RFSPEGDFVFSKSYASYNGRNDNAFDLVADNDGNLFGLSASEDNVAPYSQHIAVLKLN
                     TNNGKAIFIKRIGEGAAGSEAIYGASGNTQGNGLVFAGSASDGAGGRNGKIWRLNSSG
                     TTMWSKTLNETSPGTVEECRAVAFHPESGMVYATNPAGGLIIMYCLNSSTGVNVYAPY
                     EYDGPGTTQNLPMDIKFNSLADVIISSVAQSGLNSEFQLVKITEMELRQENAQNNIEL
                     SCYPNPVADYLVINGIEEDCLLQIVNNAGQLMLESSLSVGNNKIDVQTLPSGQYYVIC
                     LTNTGLHQGNFIKLK"
BASE COUNT          429 a          273 c          217 g          448 t
ORIGIN      
        1 gtcttaaaac cttccaggtt taaacctgga aggttttagg gactctgaca aatttgcagc
       61 ccgggaaaac ccaaaataac accctcaaaa tcgggttaga aattgtaata gaaaaccttt
      121 aaacttccta tcaaggttga tcatttaata agttactcag caatatgttt gtagatgaat
      181 tttgaacgtt ctgaacaaaa tatcagatat cattcccttt gcctaattaa aacgaaaacc
      241 gattacaact ttgcagtcat aaccggtttt tcctgtttgt aatgaataaa cgataaaggc
      301 ttgatttatt tttacattat tttaatttta taaaattgcc ctgatgtaaa ccagtgtttg
      361 taaggcaaat tacataatac tgtcctgaag ggagcgtttg cacatcaatt ttattatttc
      421 caacactgag tgatgattca agcataagtt gacctgcatt attaacgatt tgtaataaac
      481 aatcttcttc aataccatta attaccagat aatcagcaac cggatttgga taacaggata
      541 attcaatatt gttttgggca ttttcctgac gcaattccat ttccgtaatt tttaccagct
      601 ggaattcgga gtttaaacct gattgtgcca cgctagaaat aattacgtcg gctaaactat
      661 taaatttaat atccataggc aggttctgcg ttgttcccgg gccatcatat tcataaggtg
      721 catatacatt tacccctgtt gaagaattta ggcaatacat aataattaat ccacctgcgg
      781 gattggtagc atataccata cccgattccg gatgaaatgc aactgctcga cattcttcta
      841 cagtacccgg agatgtttca tttagggttt ttgaccacat ggtagttccg ctgctattta
      901 atcgccaaat tttaccattt cgtccacctg ctccatcaga tgcacttcct gcaaaaacta
      961 atccattacc ttgtgtgttt ccggatgctc cataaatagc ttctgaacct gcggcgcctt
     1021 caccaatacg ttttataaat atggccttgc cgttatttgt atttaatttt aaaacggcaa
     1081 tatgttgact atatggagca acattatctt cagaagccga taatccaaat aaattgccat
     1141 cattatctgc aactaaatca aatgcattat cattccgtcc attataactc gcataagatt
     1201 tactaaaaac gaaatcacct tcaggactga aacgggctaa aaacatatcc ttgtttgttc
     1261 cggccggatt tttatcataa ttaccccaga taaaaatatt ggaagcggaa ttaatgtgca
     1321 ttgctttcgc atatatattt tttccggaaa acatatatgc gatttct
//
LOCUS       DAORKW010000754         1350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_7526_length_1350_cov_5.254826, whole genome shotgun sequence.
ACCESSION   DAORKW010000754 DAORKW010000000
VERSION     DAORKW010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7526_length_1350_cov_5.254826"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLB46_17575"
     CDS             complement(<1..489)
                     /locus_tag="PLB46_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRF78395.1"
                     /translation="MSKVYVAIMAGGIGSRFWPESRVKKPKQFLDILNTGETLIQTTY
                     NRFLKIADKDNIFIVTNEQYVPLINEQLPEIDASRILAEPMRKNTAPCIAYACHKIAS
                     MDPDANIVIAPSDHIMLNPDKFLYVVLEALEYTQKNDVLVTLGIKPTRPDTGYGYIQF
                     EEG"
     gene            652..>1350
                     /locus_tag="PLB46_17580"
     CDS             652..>1350
                     /locus_tag="PLB46_17580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRF78396.1"
                     /translation="MEVSNMTVKEALQEFFGFDSFKGNQEQIIESLLKGQDTFVIMPT
                     GGGKSLCYQLPAMMLEGTAIIVSPLIALMKNQVDLVRGYSSNNDIAHFLNSSLNRQQI
                     KKVKQDITEGRTKMLYVAPETLRKEENIEFFKGIKVSFVGVDEAHCISEWGHDFRPEY
                     RQIHTMIETINDDIPIIALTATATPKVQSDIKKNLGLENPNVFMSSFNRPNLYYEIRP
                     KRSKEFAIKQIVKYI"
BASE COUNT          433 a          251 c          269 g          397 t
ORIGIN      
        1 gccctcctca aattgtatat aaccataacc ggtatcggga cgagttggtt taatacccaa
       61 agtaaccaat acatcgtttt tttgagtgta ttccaatgct tctaaaacga catataaaaa
      121 tttgtcggga ttgagcataa tgtggtcgct tggggcaatc acaatattag catcagggtc
      181 catactggca attttgtgac aagcgtatgc tatacacgga gccgtgtttt ttcgcatcgg
      241 ttctgccaaa atccttgaag catcaatttc cggcaattgc tcgttaatta atgggacata
      301 ttgttcattt gttacgatga aaatgttatc tttgtcagct atttttaaaa acctgttgta
      361 ggtagtttga attagggttt cgccggtgtt gagaatatct aaaaactgtt taggtttttt
      421 tactctgctt tcaggccaaa acctgcttcc aatacctccg gccattatag ccacataaac
      481 tttactcatt gaagcggatg ataaatttct actaagagaa ctgttttata aaattttatt
      541 atatttagaa gacgattcta atgtcggaaa agtttgcggc agaattttat tgtgccttga
      601 ctttacgacg caaatatacc ttaaaagaca ttgataaaaa caaattgaga gatggaagta
      661 tctaatatga cagtgaagga agccttgcag gagttttttg gtttcgactc gtttaaagga
      721 aatcaagagc aaataattga aagtttgtta aaaggacagg acaccttcgt aatcatgcca
      781 acaggaggag gaaagtcact gtgttaccaa ttgcccgcaa tgatgctgga aggaacggca
      841 attatagttt cgccacttat cgctttaatg aaaaaccagg tagatctggt tagaggatat
      901 agcagtaaca acgatatcgc tcacttttta aactcttcct taaacaggca gcagattaaa
      961 aaagtaaagc aggacattac cgaaggacgc accaaaatgt tatacgtggc gcctgaaacc
     1021 cttcgtaaag aggaaaatat cgaatttttt aaagggataa aagtttcttt tgttggtgta
     1081 gatgaagcgc attgtatttc agaatggggg cacgacttcc gtcctgaata ccgccagatt
     1141 cataccatga ttgaaaccat caacgatgat attcctatca ttgcccttac tgctacggcc
     1201 actccaaagg tgcagagtga tattaaaaag aacctcggac ttgaaaatcc gaatgtcttc
     1261 atgtcgagct tcaacaggcc taacctctat tacgaaatac gcccaaaacg cagcaaagag
     1321 ttcgctatca aacaaatagt gaagtatata
//
LOCUS       DAORKW010000755         1345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_7577_length_1345_cov_4.477519, whole genome shotgun sequence.
ACCESSION   DAORKW010000755 DAORKW010000000
VERSION     DAORKW010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7577_length_1345_cov_4.477519"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLB46_17585"
     CDS             <1..500
                     /locus_tag="PLB46_17585"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78397.1"
                     /translation="ARLNQLKHSGLMYNGTYSPNGKYIVGSSTNGDVTIWLNDSFTKL
                     HEVKTSNGAIYQCAFGPDNVTFAAVGESKEIYIIAAGSGKILRKIPAHNERIYSVAWS
                     PDGSRIATGSSDNTVRLWDVNTDQTTLIIADFKEDIYHVIFSSDNTALYVNSPGSGFK
                     KYATN"
     gene            563..1306
                     /locus_tag="PLB46_17590"
     CDS             563..1306
                     /locus_tag="PLB46_17590"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRF78398.1"
                     /translation="MMTEAATVFDNIVKQRRSMRVYDANKPFDGEAVHRSLQRAMYAP
                     NSSNMQMWEFYRVTDPEKIKQLAKYAMNQSAARTARELVLFVVPKSKWKQRAKANFEF
                     HHSRYGNKPLHELSKREQRKLKYFTRLMPMYYFQDWLGIWGMIRKLIVAISSLWRPVV
                     WQVTKNDLRVVCHKSAALAAQTFMLSMKAEGYDTCPMEGFDSLRVKRYLNFPSSYEIN
                     MMISCGPGTAEGIYHDQFRVDEKEIIFTV"
BASE COUNT          426 a          223 c          279 g          417 t
ORIGIN      
        1 gtgcgcgatt aaaccaacta aagcatagcg gactcatgta taacggtacg tattctccaa
       61 atggaaaata tattgtcggt tcatccacaa atggagatgt tactatttgg ctgaacgatt
      121 catttacgaa attacatgaa gtaaaaactt caaatggcgc tatatatcaa tgtgcgtttg
      181 gacctgataa tgttacattt gctgccgtag gggagagtaa agaaatttat attattgcag
      241 caggctcagg taaaattctc cgcaaaattc cggctcataa cgaacgcatt tattcagtag
      301 cctggagccc agatggcagc aggattgcta ccggttcatc cgataataca gtgcgcttgt
      361 gggatgtaaa tactgaccaa acaactttaa taatcgcaga cttcaaagaa gatatttatc
      421 atgtgatttt tagcagtgat aatacagcat tgtatgtaaa ttcacctggc agtggattta
      481 agaaatatgc cacaaattga taatacttac tcattatttt tattgtcatg tttggtagtg
      541 ttgcattttg tattttcgct gcatgatgac agaagcagca accgtttttg ataacatcgt
      601 taagcaacga cgcagtatgc gcgtgtatga tgccaacaaa ccttttgatg gcgaagccgt
      661 acatcgcagt ctgcaacgtg ctatgtatgc tccaaatagc agtaatatgc aaatgtggga
      721 attttatcgt gttactgacc cggaaaaaat aaaacaactt gctaaatatg ccatgaatca
      781 aagtgctgcg cgaactgcac gtgaactggt attgtttgtt gtgcctaaat caaaatggaa
      841 acaacgcgcc aaagccaatt tcgaatttca tcattcgcgt tatggtaata aaccgcttca
      901 cgaattatcg aaacgagagc aacgtaaatt aaaatatttt acccgtttaa tgccaatgta
      961 ttatttccag gattggttag gaatttgggg gatgataaga aaattaattg tcgccatttc
     1021 gtcgttatgg cgacctgtgg tttggcaggt aacaaaaaat gatttgcgtg ttgtttgtca
     1081 taaatcagct gcattggctg ctcaaacatt tatgttgagt atgaaagctg aagggtatga
     1141 tacctgtcct atggaaggtt ttgattcttt gcgggtaaaa cgttatttaa atttcccttc
     1201 cagttacgaa attaacatga tgattagttg tggtccgggt acagcagaag gtatttatca
     1261 tgaccaattc agagtagatg aaaaagaaat aatttttact gtttaagggc gatatattta
     1321 gttaaaaaaa ttaattataa ttttt
//
LOCUS       DAORKW010000756         1333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_7712_length_1333_cov_4.392801, whole genome shotgun sequence.
ACCESSION   DAORKW010000756 DAORKW010000000
VERSION     DAORKW010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7712_length_1333_cov_4.392801"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLB46_17595"
     CDS             <1..812
                     /locus_tag="PLB46_17595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78399.1"
                     /translation="MDMSSLAYSMVDIKFTDNLHGFVTGQAADLAQGGIILYTENGGD
                     SWTTKVTTGHPNDYVWKIQLLDGVHAYGAIADVTGTSTTRFLKSLDNGNSWEVKLIDE
                     AYVYIEMIGFANPDTGWTGSYDILETTDGGETWHNTDWGYNINRFFKVNDNLSFASGS
                     TIYVYADTTYVPVDTTTGIEDFVHNHSIIDISPNPVTTSLSATIQIDVVTFADICVYD
                     MKGIKQLTLYHDKIQPGKTTFNQQINLPAGQYVLSIHSNEGLRWKKFIVVN"
     gene            complement(838..>1333)
                     /locus_tag="PLB46_17600"
     CDS             complement(838..>1333)
                     /locus_tag="PLB46_17600"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78400.1"
                     /translation="LSAVLSTCSGSLLAPASILAENFIKPIHKTIKKTEYTDKHFLLV
                     LRLSVIAIAIVSFLMTMSKQNIYELVGESATFGMVSLLAPMTAGLYWKNVNAQSAMLS
                     MFSGLITYVIFEYIYPIQIEAFIPATIISFIAMYAGRFIEDKKAPANYASGLASRFMK
                     FFKD"
BASE COUNT          446 a          220 c          241 g          426 t
ORIGIN      
        1 atatggacat gagcagtttg gcttattcga tggtggatat caagtttact gacaatttgc
       61 atggttttgt aaccggtcaa gctgctgatt tggctcaggg aggtatcatt ctttatacgg
      121 aaaatggtgg tgacagctgg actacaaaag ttacaacagg tcatcccaat gattatgtgt
      181 ggaaaattca attattagat ggcgtgcatg cctatggtgc tattgccgat gtgaccggta
      241 catcaacaac cagattttta aaatcgttgg ataatggcaa cagttgggaa gtgaaattaa
      301 ttgatgaagc atatgtgtat atcgaaatga ttggttttgc aaacccggac acaggttgga
      361 caggttctta tgatatttta gaaacaacag atggcggtga aacatggcat aatactgatt
      421 ggggatataa tatcaatcga ttttttaaag taaatgataa tttatctttt gcatcgggct
      481 ctaccattta tgtttatgct gatacaactt atgttccggt tgatacaact accggcattg
      541 aagattttgt tcacaatcac agtatcatcg atatttcacc aaatcctgtt acaacatctt
      601 tatcagcaac tattcaaata gatgttgtca cttttgcaga tatctgcgtt tatgacatga
      661 aaggcattaa gcaattaacg ttataccatg ataaaattca accgggtaaa actactttca
      721 accaacaaat aaatttaccg gcaggacaat atgtattatc cattcattcg aatgaaggat
      781 tgcgatggaa aaaatttatc gtggtaaatt aatggttgaa aatgtattta ttatacatta
      841 atctttgaag aatttcataa aacggctggc taaaccggaa gcataattgg caggtgcttt
      901 tttatcttca ataaatcgtc ctgcatacat ggcaataaat gaaattattg ttgcaggaat
      961 aaatgcttca atttgtatag gataaatata ttcaaaaata acatacgtta ttaatccgct
     1021 aaacatactc aacatggcac tttgagcatt tacgtttttc caatacaacc ctgccgtcat
     1081 aggtgctaac agcgatacca taccaaatgt tgcactctct ccaaccaatt cataaatatt
     1141 ttgtttcgac atagtcatta aaaaactgac aatggcaata gcaattacac tcaatcgcaa
     1201 tactaataaa aagtgcttat cagtatattc cgttttttta atggttttat gaattggttt
     1261 aataaaattt tcagccaaaa tactggcagg tgctaataaa gaacctgagc atgtacttaa
     1321 aactgcggac aat
//
LOCUS       DAORKW010000757         1325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_7797_length_1325_cov_4.914961, whole genome shotgun sequence.
ACCESSION   DAORKW010000757 DAORKW010000000
VERSION     DAORKW010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7797_length_1325_cov_4.914961"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PLB46_17605"
     CDS             complement(<1..805)
                     /locus_tag="PLB46_17605"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HRF78401.1"
                     /translation="MATLSAVVLNAQVTPDLIIEGNQDYTQFGYSTAAADFNNDGYND
                     LLIGSPAHAFGQSDEGVVFIHNGSAAGVSPTYSSRQKNQVQAYLGTDIDAAGDVNGDG
                     YADAVFGAPFFDNGQTNEGAVFVYWGSVFGLTTAEMTTIENNQSESYLGNQVAGAGDV
                     NNDGYDDIIIAAPNFDNGQTDEGVVYLHLGSASGISSTPTLMLEGNQASCYFGSSIAA
                     AGDLNNDGYDDILVTATAYDNGQVDEGVVFIYYGNASGVNTTPAYLLEAN"
     gene            complement(934..>1325)
                     /locus_tag="PLB46_17610"
     CDS             complement(934..>1325)
                     /locus_tag="PLB46_17610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78402.1"
                     /translation="TTTTRGNYKCRVTKTATGCYKNSNTIAVSVPCKEGLPAGEAGLP
                     GDEPGEMINNEILIYPNPANSNINIETSNNNSKEIVIYDAIGNVIISHSSSAYITQIP
                     IDMLSSGIYFIQVVDGEYSNMNNFVKQ"
BASE COUNT          413 a          284 c          216 g          412 t
ORIGIN      
        1 gattagcttc taacaaatat gcaggtgtgg tattaacacc gcttgcattt ccataataaa
       61 taaataccac accctcatct acttgaccat tatcgtaagc tgttgcagta actaaaatat
      121 catcataccc atcattattt aaatcgccgg ctgcagctat actactacca aaataacaag
      181 atgcctgatt gccctccaac attaaagtgg gtgtgctact tataccactt gccgagccta
      241 aatgcagata cacaacacct tcatcggttt gaccattatc aaagtttgga gcagcaataa
      301 tgatatcatc atacccatca ttatttacat caccggcacc ggccacctgg ttacctaaat
      361 aagattccga ttgattgttt tcgatggtgg tcatttcagc ggtggttaat ccaaaaacag
      421 aaccccaata cacgaaaacg gcaccttcat tagtttgacc gttatcaaaa aacggcgcac
      481 caaaaactgc atcagcataa ccatcaccat ttacatcacc ggcagcgtca atatcagttc
      541 ccaaataagc ttgcacctga tttttttgcc ttgaactata agtagggctc acacctgcgg
      601 ctgaaccatt gtgaataaat acgacacctt cgtctgattg tccgaatgca tgcgccggag
      661 aaccaattaa cagatcgtta taaccatcat tattaaaatc ggctgcagca gttgaataac
      721 caaattgtgt gtaatcctga ttgccttcaa taattaaatc cggggtaact tgtgcattta
      781 aaactacagc gcttaatgtt gccattacca acaaatgaga acgctttaaa aaaatctgtt
      841 taaacatatt ccgctttcgg ttaggagttt tgaatgatta tagattgtta ttagcactca
      901 tttcaaaaat acctttgagg tgagtgctag tatttattgt tttacgaaat tattcatatt
      961 actatattcc ccatccacca cttgaataaa ataaataccg gatgataaca tatcaattgg
     1021 tatttgtgta atgtatgctg atgaggaatg agaaataatt acatttccaa tagcatcgta
     1081 aataacaatt tctttagaat tattattgga tgtttcaata ttaatattgc tgttggcagg
     1141 gtttggataa attaaaattt cattattaat catctcccct ggctcgtcgc caggcaagcc
     1201 tgcctcgccg gctggtaaac cttctttaca aggcacactc accgcaattg tatttgaatt
     1261 tttataacat cctgttgctg ttttggttac acggcattta taattacctc gagttgttgt
     1321 cgtat
//
LOCUS       DAORKW010000758         1312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_7941_length_1312_cov_4.977725, whole genome shotgun sequence.
ACCESSION   DAORKW010000758 DAORKW010000000
VERSION     DAORKW010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7941_length_1312_cov_4.977725"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(341..724)
                     /locus_tag="PLB46_17615"
     CDS             complement(341..724)
                     /locus_tag="PLB46_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78403.1"
                     /translation="MKYGIFVIVLFFAGQAYGQQTRMDVRSLQHLAQSFSNQQSIQPA
                     DQPTISNLGINYGMVTTQSDVGTDIPSITSGSTDNSCDVVVQEELVAGRMGSNENKIT
                     TLSLNLDKIKGWLVALFHKKELDNN"
     gene            972..>1312
                     /gene="sucC"
                     /locus_tag="PLB46_17620"
     CDS             972..>1312
                     /gene="sucC"
                     /locus_tag="PLB46_17620"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HRF78404.1"
                     /translation="MNLHEYQGKELIKRYGVKIQEGIPVDTPEQAVEAARQLQTQTGT
                     NIWVVKAQIHAGGRGKAGGVKVAKSLDDVKEKAGNILGATLVTPQTGAKGKRVNKVLI
                     AQDVYYPGASE"
BASE COUNT          425 a          253 c          240 g          394 t
ORIGIN      
        1 tttttcaaag cgcttacaac agtcatatct gccgtttcgg ctatggcttt cgacaaatgc
       61 gaacccattt taccggttgc tccggcaata caaattttgg ttttcatcct gttcaattta
      121 ggataaaaat acaataggtt tttttaaaat ggaatgacta atgtttgtat atgaaccaaa
      181 cattattact caataattag cggatattac ttcccaattt tttcgtggaa aatttaattg
      241 acgtaagcaa ccttcctcac aaaacgggat ggtcattatt acatgcatga caaaataaaa
      301 cagctatttt ttgcagactt taaaaattag actgatggta tcagttatta tccaactctt
      361 ttttatgaaa gagtgccaca agccaaccct taatcttatc taaatttaag gacaaagtgg
      421 taattttatt ttcatttgag cccatacgtc ccgctaccaa ttcttcctgc actacaacat
      481 cacatgaatt gtccgttgaa ccactggtaa ttgatgggat atcagtcccc acatctgatt
      541 gcgttgttac cataccatag ttaattccca aattagatat ggtaggttga tctgccggtt
      601 gaatagattg ttgattagaa aacgactggg ctaagtgttg taaagagcga acatccattc
      661 ttgtttgttg tccgtaagcc tgccctgcaa agaataaaac gattacaaaa attccgtatt
      721 tcatatgcta ataacgctga tacaacaaca aaattatagt gaaatgccat accaccttca
      781 ccaaaaaccc atttttacac aattatgata ttttgtggtt tcctgcttcg ttactaaatg
      841 gctgaaaacc attgatattg gttgttttga cggttatttt gattaaactg ttaaatattc
      901 cggtaaatca acatcacaaa acatcaaact caggctgctt attaatatat ttgcgcaaaa
      961 tataaacgaa aatgaatctg cacgaatatc aaggcaaaga actcataaaa cgttatggtg
     1021 ttaaaattca ggaaggtatt cctgttgaca cacctgaaca agctgttgaa gctgcacgtc
     1081 agctgcaaac tcaaaccggc accaacattt gggtggtaaa agctcaaatc catgcgggtg
     1141 ggcgcggtaa ggcaggcggt gttaaagtgg ccaaaagcct ggatgacgta aaggaaaaag
     1201 ccggtaacat tttaggcgca acattggtaa ctcctcaaac cggggcaaaa ggtaaacgcg
     1261 taaacaaagt gttgattgct caggatgttt attatccggg agcttccgag at
//
LOCUS       DAORKW010000759         1289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_8217_length_1289_cov_4.188817, whole genome shotgun sequence.
ACCESSION   DAORKW010000759 DAORKW010000000
VERSION     DAORKW010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8217_length_1289_cov_4.188817"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PLB46_17625"
     CDS             <1..928
                     /locus_tag="PLB46_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRF78405.1"
                     /translation="RIEKLPVVDGNGILKGLITYKDILKLASHPRACKDKYGRLLVGA
                     AVGVTHDTIQRIEALRAVGADLVIIDTAHGHSKGVIDMVKTVKSKFPDLEVVAGNVAT
                     GAGAKALADAGVDAVKVGVGPGSICTTRVVAGVGVAQISAINDAANALKGTGVPVIGD
                     GGIRYTGDIVKAIVAGADTIMAGSLLAGTEESPGETIIYEGRKFKSYRGMGSVEAMEM
                     GSKDRYFQDVEDDIKKLVPEGIVGRVPFKGTLEEVMVQYIGGLRAGMGYAGAKDVASL
                     HNAQFVKITASGIRESHPHDVVITKEAPNYSR"
     gene            complement(1014..>1289)
                     /locus_tag="PLB46_17630"
     CDS             complement(1014..>1289)
                     /locus_tag="PLB46_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78406.1"
                     /translation="FAYYYDKQVFKNINTQEIYKNIQEDFAKKHIYTTNDITELDYKN
                     YNHILFLNAGADFSFPNNNIEKELSQHYSNKSTQKFYEIFIVKEFVK"
BASE COUNT          366 a          213 c          313 g          397 t
ORIGIN      
        1 ccgtatcgaa aagcttcctg ttgttgatgg caatggcatt ttgaagggat tgataaccta
       61 taaagacatc ctcaaactgg cgagtcaccc tcgtgcttgt aaagataaat atggtcgttt
      121 actggtgggc gccgctgttg gcgtaacaca tgatactatt caacgtattg aggctttgag
      181 agctgttggt gccgatttag tgattattga cactgcacac ggtcattcaa aaggggtgat
      241 tgatatggtg aaaaccgtaa aatcgaagtt tccggattta gaagtggttg cgggtaacgt
      301 tgccactggc gccggagcca aagcgcttgc cgatgccggt gttgacgcgg ttaaagtggg
      361 tgttggccct ggaagtattt gcaccacgcg cgttgttgcg ggtgttggtg ttgcccaaat
      421 cagtgcaatt aacgacgccg ctaatgcatt aaaaggcacc ggagttccgg taataggtga
      481 tggcggaatt cgatacacgg gcgatattgt gaaagcgatt gtagcaggcg ctgataccat
      541 tatggccggc agtttattag ccggaacaga ggaaagtccg ggcgaaacca ttatttatga
      601 aggccgtaaa tttaaatcgt accgcggcat gggtagcgtg gaagccatgg aaatgggttc
      661 taaggaccgt tattttcagg atgtggaaga tgatattaaa aaattggttc ctgaaggtat
      721 tgttggtcgc gttccgttta aaggcacatt agaggaagtg atggttcaat acatcggtgg
      781 tttaagagcg ggtatgggat atgcaggcgc taaagatgtt gcctcattgc acaatgctca
      841 atttgtaaaa attaccgcaa gtggtattcg cgaatcgcat ccacatgatg tagtaattac
      901 aaaagaagca cctaattata gcagataatt agcgcgttgt aatattatat agtttaattt
      961 gcgtttgaat taccgttaaa aaaataaacg gtagacagcg gcatcacttg tatttatttt
     1021 acaaactctt tcacaataaa aatttcgtaa aatttttgtg tgcttttgtt gctgtaatgc
     1081 tggctcaatt ctttttcgat attattatta ggaaaactaa agtctgcacc cgcatttaaa
     1141 aataagatgt ggttataatt tttataatcc aactcggtta tatcgtttgt cgtataaata
     1201 tgttttttgg caaagtcttc ctgaatattt ttgtaaattt cttgtgtatt aatattttta
     1261 aaaacctgtt tatcgtaata ataagcaaa
//
LOCUS       DAORKW010000760         1279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_8337_length_1279_cov_3.851307, whole genome shotgun sequence.
ACCESSION   DAORKW010000760 DAORKW010000000
VERSION     DAORKW010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8337_length_1279_cov_3.851307"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>211
                     /locus_tag="PLB46_17635"
     CDS             <1..>211
                     /locus_tag="PLB46_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78407.1"
                     /translation="STGTLQWYRQMNAPTPGLDLIGLKISSVTDGNIVIAGNVDNGQS
                     LDFMTAIYNPDGDLLWQEQYDSPDKY"
     assembly_gap    212..221
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <222..1274
                     /locus_tag="PLB46_17640"
     CDS             <222..1274
                     /locus_tag="PLB46_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78408.1"
                     /translation="DKVNFVKADVDGVFYIGGKSYGISTATNRLIKYNSASFITPPDD
                     DVYFPSSFNYFENKGQIIDSEGDPVSDVKYYTKKHSPALFFTDDKLSYAWARIDTTED
                     DTLSRIDMSFVGSNVTKTIQRATSQGGEYLNYYLAHCSDGITNIRSTNRLIIPEVYND
                     VDLEYYFDQNGIKFYLIIKPGYSEQNDPISILFEGANEVDINGSGELVITGPLGSITY
                     QVADAYQIDEVGDLEPLGWDAEYIQIDDFEIGFDISTYDDEMPLVIEMKLNGVAGGGG
                     DAGFNNTWSTLYGFDGWDQLYDVATNYKTYNPSFEQPDEIIYFAFLTYNSFFPFSFVF
                     YSPIISEFTSFVQLFF"
BASE COUNT          411 a          200 c          243 g          415 t
ORIGIN      
        1 agcaccggaa cacttcaatg gtatcgacaa atgaatgctc ctactccagg cttagattta
       61 atcggactta agattagcag tgtaacagat ggtaatattg taatcgctgg aaatgtagat
      121 aacgggcaaa gccttgactt tatgacggct atttacaatc ctgatggtga tttactttgg
      181 caagaacaat atgatagtcc ggataaatac gnnnnnnnnn ngacaaagta aattttgtca
      241 aagcggatgt agatggagta ttttatattg gtggtaaatc atacggtatt tcaactgcaa
      301 ctaacaggct aataaaatat aattctgctt cttttattac ccctccagat gacgatgtat
      361 attttccatc gtcatttaat tattttgaaa ataaaggtca aataattgat tcagaggggg
      421 atccggtatc cgatgttaaa tattatacaa aaaaacatag ccctgctctc ttttttactg
      481 atgataaatt aagctatgct tgggcgagaa tagacacaac tgaagatgac accctatcaa
      541 gaatagatat gagttttgtg ggaagcaatg tgactaaaac catccaacgg gcaacaagcc
      601 aaggcggcga atatctaaac tactatttag ctcattgtag tgatggtata acaaatatta
      661 gaagcactaa tcggctaatc ataccagagg tatataatga cgtggactta gagtattact
      721 ttgaccaaaa tggcattaaa ttttacctta tcattaaacc tggttatagt gaacaaaatg
      781 atccaatttc gatactattt gaaggtgcta atgaagttga tataaacggt tctggcgaat
      841 tagtaattac tggtccattg ggttcaatta cttatcaagt tgcagacgca tatcagattg
      901 atgaagttgg cgatttagaa ccattaggtt gggatgcaga atatattcaa attgatgatt
      961 ttgaaattgg ctttgacatt tcaacttacg atgatgaaat gccgctagtt atagaaatga
     1021 aattaaatgg tgtagctggt ggaggtggcg atgcaggatt taataatacg tggagtacac
     1081 tttatggttt tgatggatgg gatcagttat atgatgtcgc aacgaattac aaaacatata
     1141 atccgagttt cgaacaaccc gatgaaatca tttattttgc ctttttaact tacaattcct
     1201 tttttccttt ttcttttgtt ttctactccc ctattatttc tgaatttact tctttcgtac
     1261 aacttttttt ttaagaacc
//
LOCUS       DAORKW010000761         1274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_8397_length_1274_cov_3.904840, whole genome shotgun sequence.
ACCESSION   DAORKW010000761 DAORKW010000000
VERSION     DAORKW010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8397_length_1274_cov_3.904840"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="PLB46_17645"
     CDS             complement(<1..1161)
                     /locus_tag="PLB46_17645"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HRF78409.1"
                     /translation="MKNSILWFCRLFTGALFIFSGLVKANDPVGFGIKLEEYYDVFAE
                     RWSWTSFLFKADWLIESVNLQAAFLTSLEVALGILLILGIWKNLTTWLLLLLILFFTW
                     LTGYSAITGSVTDCGCFGDFIPLTPLQSFYKDLVLLVLILIIFILRKNIKPILSPIAG
                     FTIAALVTGFAVYVNAHVLKHDVFLDWRPYAVGDSIVENMQLPPNPKKDVVELNYVYT
                     NKKTGETVELAFLNTELEDKTKLAQLTKYSSDKENWNLDTSFTKVKVKGDRAKISDFA
                     VSDEQGDFITDQILSNPDYTLMVVSASFDHTSKLGWEKINAMQKDAEKEGVFTYALVG
                     EGRDEIEKFRHNNQTAFPFYTGDYKVCLTIARTNPNVVLLKEGVVIDKWAWRD"
     gene            complement(1151..1264)
                     /locus_tag="PLB46_17650"
     CDS             complement(1151..1264)
                     /locus_tag="PLB46_17650"
                     /inference="COORDINATES: protein motif:HMM:NF019282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide modification associated
                     domain-containing protein"
                     /protein_id="HRF78410.1"
                     /translation="MEENDGKTLISEGVASHYMDMINYSIFALIKLSENEK"
BASE COUNT          419 a          254 c          197 g          404 t
ORIGIN      
        1 atcgcgccat gcccatttat cgatgacaac accttctttt aataaaacaa catttggatt
       61 agttctggca atggttaaac acactttgta atcgccggta taaaatggaa atgctgtttg
      121 attgttgtga cggaattttt ctatttcatc acgaccttca ccaactaaag cgtaagtaaa
      181 tacgccttct ttttcagcat ccttttgcat ggcatttatt ttttcccaac ccaatttact
      241 agtgtgatca aaactcgcac taaccaccat taaggtataa tcgggattac ttaaaatttg
      301 gtctgtaata aaatcaccct gctcatcaga aacagcaaag tcgctgattt tagctctgtc
      361 gcctttcact tttactttcg taaatgaagt atccaaattc caattttctt tatcggaaga
      421 atatttggtt aactgagcga gtttcgtttt atcttctaat tccgtattta aaaatgcgag
      481 ttcaacagtt tcacctgttt ttttattcgt gtaaacataa ttgagttcca ccacatcctt
      541 tttggggtta ggtggcaatt gcatattttc tacaatacta tcaccaacag cataaggacg
      601 ccagtctaaa aatacatcgt gttttagcac gtgcgcattc acataaaccg caaacccggt
      661 aactaatgct gcaatggtaa atccggctat tggcgacaat attggtttaa tattttttct
      721 taaaataaat atgattaaaa tgagcactaa caataccagg tctttataaa aagattgtaa
      781 tggcgtaagc ggaataaaat caccaaaaca accacagtca gtaacagaac ctgtaattgc
      841 agaatatccg gtaagccagg tgaaaaataa aattaataag agtaataacc aggtggtcag
      901 gtttttccag atacctaaaa taagcagaat acctaatgct acttcaagcg atgtgaggaa
      961 agcagcttgt aaattaaccg attcaattaa ccagtcagct ttaaataaaa aacttgtcca
     1021 gctccatctt tcagcaaata catcgtaata ttcttcgagc ttaatgccaa aacctacagg
     1081 atcgtttgcc tttaccaatc ctgaaaaaat aaataacgct cccgtaaata aacggcagaa
     1141 ccacaaaata ctatttttca ttttcactaa gttttatgag tgcgaatatc gagtagttaa
     1201 tcatatccat ataatgtgag gccacacctt cagaaataag tgttttgcca tcattttctt
     1261 ctatttgttt tacg
//
LOCUS       DAORKW010000762         1259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_8588_length_1259_cov_4.918605, whole genome shotgun sequence.
ACCESSION   DAORKW010000762 DAORKW010000000
VERSION     DAORKW010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8588_length_1259_cov_4.918605"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLB46_17655"
     CDS             complement(<1..523)
                     /locus_tag="PLB46_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRF78411.1"
                     /translation="MAKTTKQAKKTNEVKPTADTKKKKPEVTVKASPKAEKIIKPENV
                     DLGNDADRKVIKHIAVAGNIGAGKTTLTQLLAKHFNWETHFEDVENNPYLYDFYQDMP
                     RWAFNLQIYFLNSRFKQILDIQKGSKTIIQDRTIYEDAQIFAPNLHAMGLMSTRDFQN
                     YTEIFNSITGLVFP"
     gene            complement(594..>1259)
                     /gene="trpS"
                     /locus_tag="PLB46_17660"
     CDS             complement(594..>1259)
                     /gene="trpS"
                     /locus_tag="PLB46_17660"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRF78412.1"
                     /translation="KIKIHKENINAGLLTYPVLMAADIIIHRAHKVPVGKDQEQHLEM
                     TRNFAKRFNSRYEVDFFPEPIAYNFGDELVKVPGLDGSTKMSKTGGETNAIFLNEEPE
                     SIRKKIMRAKSDGGPTTMNQEKPQEIINLFELMSIVSTPETVAFYDNAYNNCVIRYGD
                     MKKQLAEDMINYLTPIRTRINDLIKDEAYLHKVVSRGAEEARESAQKTISEVRKIMGI
                     SYF"
BASE COUNT          335 a          225 c          227 g          472 t
ORIGIN      
        1 gagggaatac caagccggta atggaattaa atatttcggt ataattttga aaatcgcgtg
       61 tgctcattaa acccattgca tgtaaattgg gtgcaaatat ttgcgcgtct tcgtaaatag
      121 ttctatcctg tataattgtt ttacttcctt tttgaatatc taatatttgt ttgaagcgac
      181 tgttgaggaa ataaatttgc aagttgaatg cccaacgagg catgtcctga taaaaatcgt
      241 ataaataagg attgttttct acatcttcaa aatgtgtttc ccaattaaag tgtttagcca
      301 gtagttgagt aagtgtagtt ttaccagcgc cgatattacc ggcaacagct atatgtttaa
      361 taactttacg gtctgcatca tttcctaaat cgacgttctc cggcttaatt attttttctg
      421 cttttggtga ggcttttact gttacttcag gttttttctt tttagtatct gcagtaggtt
      481 tcacttcatt ggtctttttt gcctgttttg ttgtttttgc cattgtgttc gtcgttaata
      541 ttgattagag tagtctggat tgcaaatttg agtaaagaaa ttgtaaagga ggcttaaaag
      601 taactaattc ccataatttt tcttacttct gaaatggttt tctgcgcact ttctctcgct
      661 tcttcagcgc cacggctcac aactttatgg agataagcct catcttttat caaatcattt
      721 attctggtgc ggataggagt gaggtaatta atcatgtcct ctgccagttg ttttttcata
      781 tccccgtaac ggataacaca attgttataa gcattatcgt aaaaagctac agtttcagga
      841 gtgctcacta tgctcatcaa ttcgaacaaa ttgataattt cctgaggttt ttcctgattc
      901 atggttgttg gaccgccatc tgatttagcc cgcataattt ttttgcggat tgattcaggt
      961 tcttcattca gaaaaatggc attggtttca ccaccggttt tactcatttt tgtcgaacca
     1021 tctaatcccg gcactttcac taattcatca ccaaaattgt aagcaatagg ttctgggaaa
     1081 aaatcaactt cgtatcttga attaaatcgt ttcgcaaaat tgcgtgtcat ttcaagatgt
     1141 tgttcctggt ctttacctac aggcacttta tgcgctctat gaataataat atcagctgcc
     1201 attaaaaccg gataagtaag taatccggca ttgatatttt ctttgtgaat tttaatttt
//
LOCUS       DAORKW010000763         1250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_8713_length_1250_cov_5.083682, whole genome shotgun sequence.
ACCESSION   DAORKW010000763 DAORKW010000000
VERSION     DAORKW010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8713_length_1250_cov_5.083682"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="PLB46_17665"
     CDS             <1..1244
                     /locus_tag="PLB46_17665"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HRF78413.1"
                     /translation="KDEADRIQLSADYAQLMNGLKLYKTQVDGMRRMDAVGIKEAQEK
                     EFMKWAKTQGKSTEEKYQTMFNNFENAYKNLSTVNTEFYYKIYSVVLLPTGSFALDFS
                     EVESLFGDEALQGAERTAVIDGIKESADGMWESYNYETEVKKMVALLNLMHTKLPEAK
                     QPQVIKDILAKTKGASAEEKFVTWSKNAFAKSVFTSKAKLDAYLANPSEKKLTKDPLY
                     NYYISLYTDAQTAGSVFRAANTEISKLQREYTAALMQKDPKKNFYPDANSTLRLTYGT
                     VQDYEARDAVHYHWQTTIDGVMEKRDNTDDEFKVPEKLYDLYKARDYGRYADKSGTVP
                     VCFISNLDITGGNSGSPIMNGKGELIGLAFDGNYEGTPGDYIVDPDLNRTISVDIRYV
                     LFIIDKYAGASNLINELKIAK"
BASE COUNT          445 a          193 c          245 g          367 t
ORIGIN      
        1 ataaagatga agctgatcgt attcaattat ctgctgatta tgcgcaattg atgaatggtt
       61 taaaattata caaaacacag gttgatggta tgcgcagaat ggatgctgtt ggcattaaag
      121 aagcacagga aaaagaattc atgaaatggg ccaaaactca aggcaaaagc actgaggaga
      181 aataccagac catgtttaat aatttcgaaa atgcctataa aaacttaagc accgttaata
      241 cagaatttta ttataaaatt tattcagtgg tattattacc tactggtagt tttgcattag
      301 atttcagtga agttgaatca ttatttggtg atgaggcttt acaaggtgct gaacgtactg
      361 ctgtaattga tggcataaaa gaatcagcag atggcatgtg ggaaagttat aattatgaaa
      421 ctgaggtgaa gaaaatggtg gctttattaa atttaatgca caccaaatta cctgaagcaa
      481 aacaaccaca ggttattaaa gatatattag ctaaaacaaa aggtgcaagt gctgaagaaa
      541 aatttgttac ctggagtaaa aatgcatttg caaaatctgt atttaccagc aaagcaaaac
      601 ttgatgcata tttagctaat ccaagcgaaa agaaactcac taaagatcct ttatataact
      661 attacatttc attatatact gatgctcaaa cagcaggttc tgtatttaga gctgccaata
      721 ctgaaataag caagttgcaa cgtgaatata ctgcagcatt aatgcaaaaa gatccgaaga
      781 aaaatttcta tccggatgct aactcaacat tgcgtttaac ttatggaact gttcaggatt
      841 atgaagcacg tgatgctgtt cattaccact ggcaaactac cattgatggt gtaatggaaa
      901 aacgcgataa taccgatgat gaatttaagg ttccggaaaa attatatgac ttatataaag
      961 caagagacta cggtcgttat gcggataaat caggtactgt tccggtttgt tttatttcta
     1021 acctcgacat taccggtggt aacagcggaa gccctattat gaatggcaaa ggcgaactaa
     1081 ttggtttagc ttttgacggc aactatgaag gtactccggg cgactacatt gtagaccctg
     1141 atttaaaccg caccatttct gtagatatcc gttatgtgtt atttattatt gataaatacg
     1201 ctggtgcaag caatttgatt aacgaattga aaattgccaa ataatttatt
//
LOCUS       DAORKW010000764         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_8948_length_1235_cov_4.953390, whole genome shotgun sequence.
ACCESSION   DAORKW010000764 DAORKW010000000
VERSION     DAORKW010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8948_length_1235_cov_4.953390"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLB46_17670"
     CDS             complement(<1..237)
                     /locus_tag="PLB46_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78414.1"
                     /translation="MDLQELLTVYREQDDRIKAFTGQIATAQKPINLHLKGIAGSLLS
                     FVVSGVYKNLQHNHLFILNDKEEAGYFLNDLETLL"
     gene            368..1093
                     /locus_tag="PLB46_17675"
     CDS             368..1093
                     /locus_tag="PLB46_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRF78415.1"
                     /translation="MLAWQNGIVKEVIIQTHNTRSFIIEATEQPVFDFIPGQFVTLDL
                     PISERPSQRMRSYSIASAPMGNNQFELLISHKEGGLGSTYLFEEAVEGASLRFRGPAG
                     KFVLPATLDRPIVMISTGTGIAPFRSQILHLLHHQIPVPEIHLVFGTRKESDILYHNE
                     LLDLEKMYPHFHYHVALSRHESHEWKGAKGYVHDVYETISEKGKRDMDFYLCGWRNMV
                     ADAKTRIAALGYDKERVHLEVYD"
BASE COUNT          377 a          219 c          253 g          386 t
ORIGIN      
        1 gagaagggtt tcaaggtcat ttaaaaaata accagcctct tctttatcgt tgaggataaa
       61 caggtgattg tgttgaagat ttttgtaaac gccggaaaca acgaaggaaa gtaaagagcc
      121 tgcaatgccc ttcagatgca ggtttatcgg cttttgagcc gttgctatct gtcctgtaaa
      181 tgcttttatc ctatcatcct gctcgcggta taccgtaagc aattcctgca aatccatgaa
      241 cgttctgcaa aggtattgaa tttataggta acaaggtttg ttgttttggg gttggcatat
      301 aattgacaat tattttaagg taaatcaact ttttcacgca actttgtgtt ccattcaaaa
      361 agacaatatg ttagcttggc aaaacggtat agttaaagaa gtaattattc aaacgcataa
      421 caccagaagt tttataattg aagccaccga acaaccggtt tttgatttta tacccggtca
      481 gtttgttaca ctggatttac ccatcagcga acgccctagt caaaggatgc ggagttattc
      541 aatagcttct gcccctatgg gcaacaacca atttgaatta ttaattagtc ataaagaagg
      601 cggcttaggc tccacttatt tatttgaaga agctgttgaa ggtgcttcct taaggtttag
      661 aggacctgca ggcaagtttg tattacctgc aacactcgac aggcctattg ttatgattag
      721 cacagggaca ggtattgctc cttttcgctc acagatatta catttattgc atcatcaaat
      781 accggttccg gaaatacatt tggtttttgg cacacgtaaa gaatcggata tattatatca
      841 caatgaatta ttggatttag aaaaaatgta tccgcatttt cattatcatg ttgcattgag
      901 tcgccacgaa tcacatgaat ggaaaggcgc caaaggttat gtgcacgatg tatatgaaac
      961 cataagcgaa aaaggtaaac gcgatatgga tttttattta tgcggatggc gcaatatggt
     1021 cgctgatgcc aaaacaagga ttgctgcttt agggtatgac aaagaaagag tacatttgga
     1081 ggtttacgac taacctatgg ccattatcat actgattacc ctgattgcac tttttatcac
     1141 tgcaagtgga tattttaaaa atactaaact gggtagttgg tatgcattaa taatttcgat
     1201 tgttattata agcgcctaca cggctattac tactg
//
LOCUS       DAORKW010000765         1222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_9148_length_1222_cov_4.482434, whole genome shotgun sequence.
ACCESSION   DAORKW010000765 DAORKW010000000
VERSION     DAORKW010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9148_length_1222_cov_4.482434"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLB46_17680"
     CDS             <1..350
                     /locus_tag="PLB46_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRF78416.1"
                     /translation="AIILSPDTSKKFTPIDMQAHFPLSQQFSAEQLISKFKRQREKNL
                     SSLKKFNIQKSDYALTAVHPVLGAVSLQQLLSAWVVHDLTHISQISRVMAKLYTSEVG
                     PFRQLLGVLNQKN"
     gene            595..>1222
                     /locus_tag="PLB46_17685"
     CDS             595..>1222
                     /locus_tag="PLB46_17685"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRF78417.1"
                     /translation="MKKFLKWLGIAVLAIVLVLVIAVLILVNKTNGMAKKTYDVNLPK
                     FAAANDSAALVRGAVIANSLCGGCHGGDFAGTEFFKDDATGSIPASNITSGGRTKNYT
                     DADWLRIIRYGVKPDKHGAMIMPSKEMGSMSNEDIAALVGYLKTIPASNKTWPDPEFT
                     LLSKVLAGAGLFGDLYHAEIIDLQDTTTKTAPEPGPTVAYGAYTVGFHG"
BASE COUNT          391 a          219 c          241 g          371 t
ORIGIN      
        1 cagcaataat tttatcgcca gatacttcca aaaaatttac accaatagat atgcaggcac
       61 attttccgct gtcgcaacaa tttagcgcag agcagttaat cagcaaattt aaacgacagc
      121 gagaaaaaaa tttatcgtcc ttaaaaaaat tcaacataca aaaatcagat tatgctttaa
      181 cagccgtaca tcccgtgtta ggagcggtaa gtttgcaaca attgttatct gcctgggtgg
      241 tgcacgattt aacccatata tctcaaatca gtcgcgtaat ggccaaatta tatacttcag
      301 aagtgggacc gtttaggcaa ttattaggtg tgcttaatca aaagaattaa gttccacttt
      361 tattggattt accctttaat tatttcaaat caaacttcga acaatttcac aaggaggata
      421 ggatgaatat ttaaccatca tcattatcaa acagttggta atatcatgta tttacctgcc
      481 tataatcgtg atttaaatca caaaaacaca tatggtttgt ttgaatctac cattaggttt
      541 atttttagtt aacatttgaa ccatccgcct cggtggatat aaacctaaat acacatgaaa
      601 aaattcttaa aatggctggg cattgccgta cttgctatag tacttgtatt ggtaattgcc
      661 gtattaatat tggtgaataa aaccaatggc atggccaaaa aaacctacga tgtcaattta
      721 ccgaagttcg ctgctgctaa cgattcagca gctttagtaa gaggtgctgt aattgcaaat
      781 tcactatgtg gtggctgtca cggtggtgat tttgccggca ccgaattttt taaagatgat
      841 gcaacaggca gtattccggc ttcgaatatt acttccgggg gcaggactaa gaattatacc
      901 gatgctgact ggttgcgtat tataagatat ggtgtaaaac ccgataaaca tggtgcgatg
      961 attatgccga gtaaagaaat gggttccatg agtaatgagg atattgcggc tttggttggg
     1021 tatttaaaaa ctattcctgc atccaacaaa acatggcctg atccggagtt tacattgtta
     1081 agcaaggtgt tagcaggagc aggattattt ggcgatttat atcatgctga aattattgat
     1141 ttacaagata ccacaactaa aactgcacct gaaccgggac cgactgttgc ttatggagct
     1201 tacactgttg gatttcatgg at
//
LOCUS       DAORKW010000766         1221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_9166_length_1221_cov_5.061750, whole genome shotgun sequence.
ACCESSION   DAORKW010000766 DAORKW010000000
VERSION     DAORKW010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9166_length_1221_cov_5.061750"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PLB46_17690"
     CDS             complement(<1..321)
                     /locus_tag="PLB46_17690"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRF78418.1"
                     /translation="MTPYPILIDNILHNAIDTAMSYTAFYNLTSALVADNNTSGPVKS
                     EERIQATKLNLQRLNRLNKTTQLLPEWSTLDVTKTSNLQWVVITEAWCGDGYQLVPVM
                     NKVAE"
     gene            465..>1221
                     /locus_tag="PLB46_17695"
     CDS             465..>1221
                     /locus_tag="PLB46_17695"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HRF78419.1"
                     /translation="MHAKRVIKYFAGYTIPLATGLSIYFGGIYSFITPVYAFVLLPFL
                     ELLIPVNNKNLSAVEEELVKKDKVYDWILYLHVPVQYAMLYYFLYTVTYTPLQGYELV
                     GLILSMGTCCGVIGINVAHELGHRKSKSEQFLAKMLLLTSLYMHFIIEHNKGHHKNVS
                     TTQDPSSARLNEPLYFFYFRTITGTIASAWHIQLTELKNASRPFLSWQNELLIFQVVQ
                     VLFLILNAWFFGLTSMFIFIPAAFIGILLLEAVN"
BASE COUNT          360 a          208 c          214 g          439 t
ORIGIN      
        1 ttccgctacc ttattcataa ccggcaccaa ttgataacca tcaccacacc aggcttcggt
       61 aattaccacc cattgtagat tagatgtttt ggttacatcc agggttgacc actccggtag
      121 caactgcgta gttttattta aacgattcag tctttgtagg tttagttttg ttgcctgaat
      181 acgttcttcg ctttttacag gtcctgaggt attattatcg gcaaccaacg cgcttgtcaa
      241 gttataaaaa gcagtgtaac tcattgctgt atcaatcgcg ttgtgcaaaa tattatctat
      301 taagattgga tatggtgtca taactaatag ttgaatggtt taacaataca aaggttcata
      361 tagttcgggg ctttaacagt gataattatc acccaacctg agaaaaatat taacagcaac
      421 attgaacctt ttcacatcca acaaaattct cttatttttg acctatgcat gcgaaacgtg
      481 taataaaata ttttgcaggt tatactattc ccttagcaac cggactgtcg atatatttcg
      541 gaggcatcta ctcgtttatt acgcctgttt atgcatttgt tttattgccg tttttagaat
      601 tattaattcc tgtcaacaat aagaatttat cagctgttga agaagaactt gtgaaaaagg
      661 ataaagtgta cgactggatt ttatatttac atgttcctgt tcaatatgcc atgctctatt
      721 attttttata cacagtaact tatacacctt tgcagggtta tgaattagta ggtttaattt
      781 taagtatggg tacgtgctgt ggtgtaatag gaattaatgt tgcacatgaa ttagggcatc
      841 gaaaatcaaa gtcagaacag tttttagcaa aaatgttatt gcttacttct ttgtatatgc
      901 attttattat tgagcataat aaaggccatc acaaaaatgt ttctacaact caggaccctt
      961 catccgcaag gctaaatgaa ccgttgtatt ttttttattt caggacaata acaggaacaa
     1021 ttgcatcagc ctggcatatt cagttaacgg aattaaaaaa tgcaagtcgt ccatttttga
     1081 gttggcaaaa tgagttactt atttttcaag ttgttcaagt gttgttttta attcttaatg
     1141 cctggttttt tgggttaact tccatgttta tttttatacc tgcggccttt atcggaattt
     1201 tattactgga agcagtaaat t
//
LOCUS       DAORKW010000767         1204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_9437_length_1204_cov_4.969539, whole genome shotgun sequence.
ACCESSION   DAORKW010000767 DAORKW010000000
VERSION     DAORKW010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9437_length_1204_cov_4.969539"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PLB46_17700"
     CDS             <1..626
                     /locus_tag="PLB46_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HRF78420.1"
                     /translation="KGNPALDGTALDDAIFGTLLLPAAGKPRSVDLWPIFFTGAPNLA
                     PYQLATGKGGNPLAAGKPFINNFLPNGGDMLRLNMATPVTDRTSPFFSSEGLLAAAVI
                     GLTVDPFNTTTALEFIPNMDGFPNGRRLEDDVTRIELQAVSGIVLAAIGLWYDDYDPA
                     SGESPLTRDMRDVINYTTRVEHNDAAFQTSFPYLAAPWRGTAVDYEY"
     gene            651..>1204
                     /locus_tag="PLB46_17705"
     CDS             651..>1204
                     /locus_tag="PLB46_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HRF78421.1"
                     /translation="MKNKFNILKEKLSTLLIAAMMLVPAFQASASSHREAPLIADDPL
                     ADNTDLYAFRSPDNPEMVTIIANYIPLQLPHGGPNYYTFGENIRYEIHIDNNIATLGD
                     DIIYRFTFNRTDEDPTTFFNIRLGAQNIKMTYTLEVSNDGGVSFSTIITNGAVPPPNI
                     GPRSINSGVGLGVSYQSLINSAITP"
BASE COUNT          348 a          235 c          231 g          390 t
ORIGIN      
        1 taaaaggcaa ccctgcttta gacggaactg cattggatga tgccattttc ggaacattat
       61 tattacctgc tgcaggcaaa ccgcgttcag tagatttatg gccgatattt tttaccggtg
      121 ccccgaattt agcgccttat caattagcta caggtaaagg tggaaatcca cttgctgccg
      181 gcaaaccatt tatcaataac tttttaccga atggtggtga tatgttgcgt ttaaatatgg
      241 caactcctgt tactgatcgc acaagtcctt tctttagttc agaaggttta ttggctgctg
      301 cagtaattgg tttaactgtt gatccattta atacaacaac agcattggaa tttattccga
      361 atatggatgg atttccaaac ggtcgtcgtt tagaagatga tgttacacgt atcgaattac
      421 aagcggtgag tggaattgta cttgctgcaa ttggtttatg gtacgatgat tatgatcctg
      481 cttcaggtga atcaccatta acccgcgata tgcgtgatgt aattaattac actacacgtg
      541 ttgaacataa cgatgctgca ttccaaactt cattcccata ccttgctgct ccttggagag
      601 gcactgctgt ggattatgaa tattaattcc aatcatcaaa aataaataca atgaaaaata
      661 aatttaatat actaaaagaa aagttgagta cgttgctcat cgctgcgatg atgttggtac
      721 ctgcttttca ggcgtcggct tccagtcacc gcgaagctcc gctaattgcc gatgatccat
      781 tggcagataa tacagattta tatgcgtttc gcagtcccga taatccggaa atggtaacta
      841 ttattgcgaa ttatattcct ttacaattac ctcacggcgg accgaattat tataccttcg
      901 gagaaaacat tcgctacgaa attcatattg ataataatat tgctacactt ggtgatgata
      961 ttatttatcg tttcactttt aacagaacgg atgaagatcc aactacattt tttaatattc
     1021 gccttggtgc tcaaaatatt aaaatgactt acacacttga agtaagtaat gatggtggtg
     1081 tttctttttc tactattatc acaaatggtg cagttccacc tccaaatatt ggacctcgct
     1141 caattaatag cggtgtaggt ttaggtgttt cttatcagag tttaattaac agcgccatta
     1201 cccc
//
LOCUS       DAORKW010000768         1192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_9650_length_1192_cov_4.347405, whole genome shotgun sequence.
ACCESSION   DAORKW010000768 DAORKW010000000
VERSION     DAORKW010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9650_length_1192_cov_4.347405"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>1192
                     /locus_tag="PLB46_17710"
     CDS             21..>1192
                     /locus_tag="PLB46_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78422.1"
                     /translation="MEDCWVLKLDAEGNIEWQNTFGGLVTEYLYKIHQTSDGGYILGA
                     YSASDISGNKTEATKGSYDYWLIKLDIDGNKLWEKTIGGSAADILADLNITDDGGFII
                     GGSSNSMISGDKTENPIGDFDYWVLKLDAVGNIEWQNTIGGSLGDELYFCSQTSDGGY
                     FIGGYSNSPISGDKSEPTFGADLEYYDYWVIKLKQNGDFYWENTIGGNKHEVCYNGIE
                     LADGTLVIGGYSTSNISGDKTEPRFGGPDIPDIWIVALETTGSIIWQSVIGGIQYDRG
                     NSFTPTPDGGFIISAESSSGISGNKTMPSFGLSDAWIIKINGVCNPTTELCNTLDDNC
                     NGLIDDDVVETINITAAGPTEFCQGGSVSLSATYSGTSLQWQKNGVDIPGATLASYT"
BASE COUNT          373 a          191 c          264 g          364 t
ORIGIN      
        1 aaacatctcc taactacggc atggaggatt gttgggtgct gaaattggat gcagaaggaa
       61 atatcgaatg gcaaaatact tttggcggac ttgtgactga atatttatat aaaatccatc
      121 aaacaagtga tggtggttat attttagggg cttattctgc ctcagatatt tcaggtaata
      181 aaactgaagc aaccaaaggg tcatacgact attggttgat aaaattagat attgacggaa
      241 ataaattatg ggaaaaaaca attggtggaa gtgcagctga tattttagca gatttaaaca
      301 ttacagatga cggtggtttt attattggcg ggtcttccaa ctctatgatt tccggtgata
      361 aaaccgaaaa cccaataggc gattttgatt actgggtttt gaaattggat gctgttggaa
      421 atatcgaatg gcagaatact attggtggaa gccttggcga tgaactctat ttttgctccc
      481 aaacttcaga tggcggatat tttattggtg gttattctaa ttcaccaatt tcaggcgata
      541 aatctgaacc aacctttggt gcagatttag agtattatga ttattgggtg attaaattaa
      601 aacaaaatgg tgatttttat tgggagaata ccattggtgg caataaacat gaagtctgtt
      661 ataatggtat tgaattagct gatggcactt tagttattgg tggatattca acatccaata
      721 ttagtggcga taaaacagaa cctcgtttcg gcggccccga tattccggat atttggattg
      781 tagcactcga aactacaggt tccattatct ggcaaagcgt aataggcgga attcaatatg
      841 acaggggaaa tagttttaca ccaacccctg atggtggttt tataattagt gcagaatcat
      901 catccggtat ttcaggaaat aaaacaatgc catcatttgg tcttagcgat gcatggatta
      961 ttaaaataaa tggggtatgt aatccaacca ccgaactctg caataccctc gacgataatt
     1021 gtaatggttt aattgatgac gatgtagtag aaacaattaa tattactgct gccggtccaa
     1081 ccgaattttg tcagggcggt agcgtgtcgc tttctgcgac ctactccgga acatccttac
     1141 aatggcaaaa aaatggtgtt gatattccgg gagctacatt agcatcatac ac
//
LOCUS       DAORKW010000769         1185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_9783_length_1185_cov_5.814159, whole genome shotgun sequence.
ACCESSION   DAORKW010000769 DAORKW010000000
VERSION     DAORKW010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9783_length_1185_cov_5.814159"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..>1185)
                     /gene="glmS"
                     /locus_tag="PLB46_17715"
     CDS             complement(143..>1185)
                     /gene="glmS"
                     /locus_tag="PLB46_17715"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRF78423.1"
                     /translation="MDSMRGRLIGSKNAIVLGGIKEYEKKLVNAKRIIIVGCGTSWHA
                     GLVGEYLFEDLARIPTEVEYASEFRYRNPVINEDDIIIAISQSGETADTLAAVELAKA
                     KGATIFGICNVVGSSIARASHAGSYTHAGPEIGVASTKAFTAQVTVLTMMALSIARKR
                     GSLPESKYQSIAIELSKIPEKVSKVLKSNDHIKEIAEEYMDVHNFLYLGRGFNFPVAL
                     EGALKLKEISYIHAEGYPAAEMKHGPIALIDENMPVVVVANNMSAHDKIVSNIQEIKA
                     RKGRIISIISENDTIIEGMSEHVIRIPETEEVLTPLLSVIPLQLLAYHIAVLLNRNVD
                     QPRNLAKSVTVE"
BASE COUNT          342 a          259 c          197 g          387 t
ORIGIN      
        1 ggctgctcct cattggtaca ttcgcacatt ggtacatcgg tacatcatcc ggctgctcct
       61 cattggtaca ttcgcacatt ggtacatcgg tacatcatcc ggctgtttaa tcagcacatt
      121 aacacatcag cacatcatca cattactcca ccgtcacact cttagccaaa tttctcggtt
      181 ggtctacgtt gcgattgagt aaaacggcaa tgtgataagc taaaagttgt aaaggaatta
      241 cacttaataa aggtgtgagt acttcttctg tttcaggaat acgaattacg tgttcggaca
      301 tgccttcaat tattgtatca ttttcagaaa tgatagaaat aattcttcct tttcttgctt
      361 taatttcctg tatgttactt acaattttat cgtgagcact catattgttg gcaacaacaa
      421 ctacaggcat attttcatca atcaacgcaa ttggtccatg tttcatttct gcagcaggat
      481 atccttcagc atggatatag gaaatctctt ttaattttaa tgcaccttct aacgcaacag
      541 ggaaattaaa tccgcgacct aaatacagga agttgtgtac gtccatatat tcttcagcaa
      601 tttctttgat atggtcgttc gattttaata ctttacttac cttctccgga attttactta
      661 attcaatagc aatactctga tatttacttt caggtaatga accacgtttg cgggcaatac
      721 ttaatgccat catggttaaa acggtaacct gagcagtaaa tgctttagtg gaagcaacgc
      781 ctatttcagg gcctgcatgt gtatatgaac ctgcgtgtga tgcgcgcgca atagacgaac
      841 ccaccacatt acaaatacca aaaatggtag cacctttagc ttttgctaat tcaacagctg
      901 ctaatgtatc tgcagtttca ccactttgag aaatggcgat aataatatca tcttcattaa
      961 ttacaggatt gcggtaacgg aactcacttg cgtattcaac ttcagttggt atacgtgcta
     1021 aatcttcaaa taaatattcg ccgactaatc ctgcatgcca tgaggtacca caaccaacta
     1081 ttataattcg tttggcattt actaatttct tttcgtactc tttaattcca cctaaaacaa
     1141 ttgcgttttt gctgccaatt aaacgaccgc gcatactgtc catta
//
LOCUS       DAORKW010000770         1160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_10211_length_1160_cov_5.468778, whole genome shotgun sequence.
ACCESSION   DAORKW010000770 DAORKW010000000
VERSION     DAORKW010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10211_length_1160_cov_5.468778"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1160)
                     /locus_tag="PLB46_17720"
     CDS             complement(<1..>1160)
                     /locus_tag="PLB46_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRF78424.1"
                     /translation="AVTGTNGKTTTTRLNAHIAKTMGYKVGFTTSDGIYIQNRMMEKG
                     DCTGPVSAKFVLMDPSIDYAVLECARGGLLKAGLAFDKCDISIVTNVTADHIGLKDID
                     SVEEMAKVKSVVPETTRKEGYAILNADDDLVFDMRKNLECNIVLFSMDENNPRIKAHC
                     ERGGVAAVLEDGWVSIIKGTWKIRVEKVVNIPLTLGGKAAFMIQNILPCIAAAFVQNF
                     KIEDLKLALNTFIPSPAMTPGRMNVFKFKHFEMLVDFAHNPDGFQAISNYLAKVEMHP
                     KVGVIAAAGDRRDEDIRELGRIAGRTFDEIIIRQDKNLRGRTDEEIMNLLKEGIFEMN
                     PNMPVICINPEADSIKYCIENAKKGSHITVCSDVIAEALDLIMQYKERDDQF"
BASE COUNT          326 a          253 c          186 g          395 t
ORIGIN      
        1 tcaaattgat cgtcgcgctc tttgtattgc ataattaaat cgagggcttc ggcaattaca
       61 tcggaacata ctgtgatatg tgaacctttt ttcgcgtttt cgatacaata tttaatcgaa
      121 tcggcttcag ggttaataca tattaccggc atgttgggat tcatttcgaa aataccttct
      181 ttcaataaat tcattatttc ttcatcggtg cgaccgcgca gatttttgtc ctgacgaata
      241 ataatttcat caaatgttct gcctgcaata cgacctaatt cgcgaatatc ttcatcgcga
      301 cgatcgccgg cggctgctat tacacctact tttggatgca tttctacttt tgctaaataa
      361 ttactgattg cctggaagcc atcagggtta tgcgcaaagt ctaccaacat ttcaaaatgt
      421 ttgaatttaa atacgttcat tcttcccggt gtcatggcag gtgatggaat aaatgtattt
      481 aatgcgagtt ttaaatcttc aattttaaaa ttctgcacaa atgctgctgc tatacaaggc
      541 aaaatatttt gaatcataaa cgctgcttta ccgcctaatg taagtggaat attcaccact
      601 ttttctacgc gtattttcca ggtgccttta ataatactta cccatccgtc ttccaacact
      661 gctgcaacac cgccgcgctc acaatgtgct tttatacgcg gattattttc gtccatacta
      721 aacaatacaa tattacattc gagatttttg cgcatatcaa ataccaaatc atcatctgca
      781 tttaaaatgg catatccttc tttacgtgta gtttcaggca caacagattt taccttcgcc
      841 atttcttcaa ccgaatcaat atcctttaat ccgatatggt ctgcagttac gtttgtaaca
      901 attgaaatat cacatttatc aaatgctaat ccagctttta ataatccacc gcgggcacat
      961 tctaaaacag cataatcgat tgatggatcc atcaacacaa atttcgcaga gacaggtccg
     1021 gtgcaatcac ctttttccat cattctgttt tggatgtaaa ttccatcgct ggttgtaaat
     1081 ccaactttat atcccattgt ttttgcaatg tgagcattta aacgcgtagt agttgtttta
     1141 ccatttgttc cggtaacagc
//
LOCUS       DAORKW010000771         1148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_10435_length_1148_cov_6.333028, whole genome shotgun sequence.
ACCESSION   DAORKW010000771 DAORKW010000000
VERSION     DAORKW010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10435_length_1148_cov_6.333028"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLB46_17725"
     CDS             <1..911
                     /locus_tag="PLB46_17725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78425.1"
                     /translation="GGWYWDMMYNILPANNADIYDVTTGIWTKTTLSKKRENIAVAVV
                     GDLIIFAGGTGNMGDVTNVDIYNATTNSWSVVYLPIAKSGMKSVVIGNNAYFGGGQGG
                     NANDVYVLNTLTNTWNTITMPTNVTGFSLSVINGKLYAAGGSLVPAGTYTNLVQIYDP
                     ILNSWSTQNLSIARTGVAAATVGSKGFFAGGTILYGYPTPTNTKRVDIFTAPLRLIAE
                     EIINPVFEATVYPNPSSVVLNIEIPTIYAATYATLYDLNGRAVKMMNIENNTTSMQVT
                     DLVPGNYILIIHDETGNRIVKKVMID"
BASE COUNT          382 a          174 c          228 g          364 t
ORIGIN      
        1 caggtggatg gtattgggat atgatgtaca atattttacc agctaataat gccgatatat
       61 atgatgtaac aaccggtatt tggacaaaaa ccacactttc taaaaaacgt gaaaatatag
      121 cagttgcagt tgttggtgat ttaatcattt ttgccggagg taccggtaat atgggagatg
      181 taaccaatgt ggatatttat aatgcaacta ctaattcctg gtcagtagta tatctgccaa
      241 tagcaaaatc aggtatgaaa tcagttgtaa ttggaaataa cgcctatttt ggtggcggac
      301 aaggtggaaa tgcgaatgat gtatatgtgt taaatacatt aaccaatacc tggaatacga
      361 ttacaatgcc aactaatgtt accggatttt cattaagtgt aattaatggt aaattatatg
      421 ctgctggagg tagtttagtg cctgcaggaa cttataccaa cttggttcaa atttatgacc
      481 caattttaaa cagttggtca actcaaaatt tatccattgc acgtacaggt gttgcagcag
      541 caacagttgg cagtaaagga ttttttgcag gaggaactat tctttatgga tatcctacac
      601 caaccaatac caaacgcgtt gatattttta ctgcaccatt acgccttatt gcagaagaaa
      661 ttatcaatcc tgtatttgag gctactgtat atccaaatcc ttcaagtgtg gtgttgaata
      721 ttgaaatacc aactatttat gctgcaactt atgctacatt atatgacttg aatggcagag
      781 cagtgaaaat gatgaacata gaaaataata caactagtat gcaggtaaca gatttggttc
      841 cgggtaatta catattaata attcatgatg aaacaggaaa ccgaatagta aaaaaagtaa
      901 tgattgattg atgataaaaa aatgttttat aaagcggcac tatgatgccg ctttttttta
      961 cctttattga catgaaaaaa atagtattat tcactttgag tattatttgt tttacacttg
     1021 ttaatgcgca ggtgcctaga tttaccaaat acgaaatcgg cgaaagtgga ggagctgcct
     1081 attttcctgg tgagccggaa tttagtatta gctattctga agatagctca ttggtttata
     1141 cggcagat
//
LOCUS       DAORKW010000772         1145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_10485_length_1145_cov_4.144037, whole genome shotgun sequence.
ACCESSION   DAORKW010000772 DAORKW010000000
VERSION     DAORKW010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10485_length_1145_cov_4.144037"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLB46_17730"
     CDS             complement(<1..744)
                     /locus_tag="PLB46_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78426.1"
                     /translation="MQTKLLLLLCGLVISNFVTAQYNLVENYSFEDYNDCPVSISGFS
                     YDGDYYCDDWYAASGGTPDYYNACAPGFTQVSVPTNLFAADQPARTGNAYGGFWTDLY
                     DNNSFLYREYVQRELAEPLVAGTCYYVEFWSAPAVQSDFLEADHATTDAIGAYFATEK
                     IGSPGISDVLDVTPQIENNGTGNYINPPGEWTKICGFFEATGGESWVAIGNFHPDDEV
                     EVVAYDGGVLGANPLVYLFIEDVLVSPVDS"
     gene            complement(808..>1145)
                     /locus_tag="PLB46_17735"
     CDS             complement(808..>1145)
                     /locus_tag="PLB46_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRF78427.1"
                     /translation="NTNQKVYPGMIIQYTVSPVLNIPMRWVTEITHVKDLHYFVDEQR
                     VGPYRIWHHEHHLKQIEGGVEMTDIVTYQPPMGILGSLANFLFIRKQLQSIFEFREKK
                     LIELFGEMR"
BASE COUNT          347 a          250 c          194 g          354 t
ORIGIN      
        1 actatctacc gggctaacca aaacatcttc aataaataaa taaaccaatg gatttgctcc
       61 taataccccg ccatcgtaag caacaacttc tacttcatca tcaggatgaa aattgccgat
      121 tgccacccaa gactctcctc ctgttgcttc aaaaaatcca caaattttcg tccattctcc
      181 tggagggttg atgtagttgc cggttccatt attttcaatt tgtggtgtta catccagcac
      241 atcgctaatt ccgggactac caattttttc tgttgcaaaa tatgcaccta ttgcatctgt
      301 tgttgcatgg tccgcttcca aaaaatcaga ttgaactgcg ggagcactcc agaattctac
      361 ataatagcag gttcctgcaa ctaaaggctc cgccaattct cgttgcacat attcgcggta
      421 taaaaacgaa ttgttatcgt ataaatccgt ccaaaatcca ccataagcat tacccgttct
      481 tgccggttga tctgctgcaa ataaatttgt aggtacactt acttgtgtaa accccggcgc
      541 gcaggcatta taataatcag gtgttcctcc actagctgca taccagtcgt cacaataata
      601 atcgccatca tatgaaaaac ctgaaatgga tactgggcaa tcgttataat cctcgaaact
      661 gtaattttcg acaagattat attgcgcagt cacaaaattg cttattacca agccacataa
      721 aaggaggagt aattttgttt gcattggttg tctttaactc aaatataggt aattcgattt
      781 acaaccaaca aaaacacatt ttggcgttta tcgcatctca ccaaatagtt caatcagctt
      841 cttttcacgg aattcgaata tcgattggag ttgtttgcgg ataaataaaa aattggctaa
      901 actacctaaa atgcccattg gtggctgata ggtgacgata tcggtcattt ctacacctcc
      961 ttcaatttgt ttgagatgat gttcgtgatg ccaaattcga taaggtccaa ctcgttgttc
     1021 atcgacaaaa tagtgtaaat ctttaacatg tgttatttca gtaacccaac gcatgggaat
     1081 atttaataca ggactgacgg tatattgtat tatcattccc ggataaactt tttgattagt
     1141 attat
//
LOCUS       DAORKW010000773         1137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_10638_length_1137_cov_3.406654, whole genome shotgun sequence.
ACCESSION   DAORKW010000773 DAORKW010000000
VERSION     DAORKW010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10638_length_1137_cov_3.406654"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1137
                     /locus_tag="PLB46_17740"
     CDS             <1..>1137
                     /locus_tag="PLB46_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78428.1"
                     /translation="AQMTYYNIDPDATVFGDIDYPIDLNLDGIPEMMMHGNRWWTFTS
                     GDVFSSQQISFINYGEIATSAGPLQLYDGDTVNADLDFNDDNQSFFNGYNWDGALDFD
                     AGTSWLNNDSYIGMRFEVDGNNHYGWIRVRLHQTDVSDMPGLIVKELAWNATPDAPAP
                     INLHVGTAQFPTLADVGETNTAADLQLHFQKPDDESNVVVYRVILYSGYDDPTLAEAN
                     AVTAERYTQVLPTGDDITINFNETTLDINGNPLISGTYYQAIILSIPDGVIVTGRDLS
                     FPSNTMNLIIREAPVAEDIYLNSTGNEDDISGFYGSFTLDETNVSSVRAYLTDGFELV
                     EELLLLDSDYYMELTPVVGENLVSFSADKLIYTSGAPVLFETYY"
BASE COUNT          334 a          203 c          234 g          366 t
ORIGIN      
        1 ctgcccaaat gacatattat aacattgacc ctgatgctac agtttttggt gatatcgatt
       61 atcctattga tttaaatctt gatggcattc cggaaatgat gatgcatggc aatagatggt
      121 ggacgtttac cagtggcgat gtattctctt cacagcaaat cagctttatt aattatgggg
      181 aaatagcaac atcggcaggc cctctacaat tgtatgacgg cgatactgtg aatgcagact
      241 tggattttaa cgatgataac caatcatttt ttaatggtta caattgggat ggagcgcttg
      301 attttgatgc cggtacctct tggctaaata acgacagtta catcggtatg cgctttgaag
      361 tagatggcaa taatcattat ggatggatac gcgtaagact gcatcaaact gatgtaagtg
      421 atatgccggg attaattgta aaagaacttg cttggaatgc cacgccagat gcccctgcac
      481 caattaattt acacgttggc acagcgcaat ttcctacctt ggctgatgtt ggtgaaacaa
      541 ataccgcagc cgacctgcaa ttgcattttc aaaaaccgga tgatgaaagt aatgttgttg
      601 tttacagggt gattttatat tccggttacg atgaccccac gcttgctgaa gcaaatgccg
      661 ttactgccga acgatacact caagtgctgc cgacaggtga cgatatcacc ataaatttta
      721 atgaaactac gctagatata aatggcaatc cgctaatttc cggtacttat tatcaagcta
      781 ttattctttc catacctgat ggcgttattg taacaggaag agacctttca ttcccatcaa
      841 atacgatgaa cttaattata agagaagcac ctgttgctga agatatttat ttaaattcta
      901 ctggtaatga agatgatatt tccggttttt atggaagttt tacattggat gaaactaatg
      961 tatcatctgt gcgcgcatat ttaactgatg gttttgaatt agtagaagag ttactgcttt
     1021 tagacagtga ttattatatg gaattaactc ctgttgttgg tgaaaactta gtatcatttt
     1081 ctgccgataa attaatttat acttctggtg caccggtgct gtttgaaaca tattatt
//
LOCUS       DAORKW010000774         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_11053_length_1117_cov_3.354991, whole genome shotgun sequence.
ACCESSION   DAORKW010000774 DAORKW010000000
VERSION     DAORKW010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11053_length_1117_cov_3.354991"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..>1117)
                     /locus_tag="PLB46_17745"
     CDS             complement(138..>1117)
                     /locus_tag="PLB46_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78429.1"
                     /translation="SGRGTTVMEGALHGRNIIANDVNPISAILAEGRLCIPDLQVLHN
                     RLDTIFAEPVQHEKNHLHMFYHKDTFRELLVLRKWIITREKNGKLDAIDKWIRMVATN
                     RLTGHSPGFFSVYTLPPNQAVTRERQILINKKRNQKPEYRNIKALIVKKTKSLIKDLQ
                     PDDIKHIHAAAKKAIFIQGDAANTTSIKSKSVQLTVTSPPFLDVVQYARDNWMRCWFN
                     GIEMKSIEKKITMSKTIEEWNIKMSSVFNELFRITKKGGYVAFETGEVHLGKTQLEEH
                     VLAMGLNAGFKCDKIIINQQQFTKTANIWGIKNNVNGTNSNRIVLFTKP"
BASE COUNT          301 a          226 c          205 g          385 t
ORIGIN      
        1 taattaaaaa aggcaactct tctaaatcaa actgtcgcgc aagcgtcagt ttctcacgta
       61 tttaatataa aaaaatcagc gcctgcctgc ctttggcagg aaaatccgcg ccatccgcgt
      121 catccgcgtt cccttgttca gggtttggtg aagagcacga tgcggttgct gttggtgccg
      181 tttacgttgt ttttgatgcc ccagatattg gctgtttttg taaattgttg ttgattgatg
      241 atgattttat cgcatttaaa tccggcattt aatcccatcg ctaaaacatg ttcttccaat
      301 tgtgtttttc cgagatgtac ttcaccggtt tcgaatgcca cataaccacc ttttttagta
      361 atacgaaata actcattaaa taccgacgac atttttatat tccattcttc gatcgttttc
      421 gacatggtga tttttttttc gatggatttc atttcaatac cattaaacca acagcgcatc
      481 caattatcac gtgcatattg caccacatct aaaaatggcg gagaagttac agtaagttga
      541 accgatttac ttttaatact tgtagtattt gctgcatcac cctgaataaa tattgccttt
      601 tttgcggcag catgaatatg tttaatatca tcaggttgta aatcctttat cagacttttt
      661 gtctttttta caataagtgc cttaatattg cgatactccg gtttttgatt gcgtttttta
      721 ttgattaaaa tctgacgctc acgggtaact gcctgattag gaggaagtgt atatacagaa
      781 aaaaatccgg gtgaatgtcc ggtaagtcgg ttggtagcta ccatacgtat ccatttatct
      841 atggcatcta attttccatt tttttcgcgg gtgataatcc atttgcgcaa cacgagtaat
      901 tcgcgaaatg tatccttatg ataaaacatg tgcaaatgat ttttttcatg ttgcaccggt
      961 tctgcaaaaa tggtatccaa acgattgtgc aaaacttgta agtccggaat acataaacgt
     1021 ccttccgcaa ggatagctga gataggattt acatcgttgg caataatatt tctgccatgt
     1081 aatgcgcctt ccattacggt cgttcctctc ccactca
//
LOCUS       DAORKW010000775         1097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_11503_length_1097_cov_3.675624, whole genome shotgun sequence.
ACCESSION   DAORKW010000775 DAORKW010000000
VERSION     DAORKW010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11503_length_1097_cov_3.675624"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLB46_17750"
     CDS             <1..450
                     /locus_tag="PLB46_17750"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRF78430.1"
                     /translation="GFIALSCGIAGGAEAIVIPETETNIDNLISILQNGWQHQKSSAI
                     VIVAEGDERGGAFEIAKLVKEKFDYYDTRVSVLGHIQRGGNPTVRDRILGSRLGMAAV
                     EALADGKKNVMAGLINDELAFTPFANATKHHMDINKQMLKLVEILAL"
     gene            complement(452..>1097)
                     /locus_tag="PLB46_17755"
     CDS             complement(452..>1097)
                     /locus_tag="PLB46_17755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78431.1"
                     /translation="FNGFDYEGITSVEKLSLSGDSWVDFDPIPEPQSYIKAFAFYWFD
                     YDVYMPCMLVPGGTNASGFGVDLTFSGTYAYDFVVQEWIEVPMFFHDRYYYAADQNQQ
                     DFIGDIIVTGGEGGTVELFKATEFININNALNNTLNLSPNPADNYINITLSAEKEVNN
                     IEVFNMMGDVVMQINNVYPTQNKIALDIHHLPAGNYVVKANGNNQITSGTFIKE"
BASE COUNT          364 a          194 c          195 g          344 t
ORIGIN      
        1 ggatttatag cattatcatg cggaattgcc ggaggtgcag aagccattgt aattccagaa
       61 actgaaacta atattgataa tctcatcagc attttacaaa atggatggca acatcaaaaa
      121 tcatcggcaa ttgtaattgt tgcagaaggt gatgaacgcg gtggtgcatt tgaaattgca
      181 aagctggtga aagaaaaatt tgattactac gatacacgcg tttcggtgct cggacatatt
      241 caacgtggtg gtaaccctac agtgcgcgac cgtattttag gcagtcgttt gggtatggca
      301 gcagttgaag cattagccga cggcaaaaaa aatgtaatgg ccggtttaat taatgatgaa
      361 ttagcattta caccatttgc aaatgctacc aaacatcata tggacatcaa caaacaaatg
      421 ttgaagctgg ttgaaatttt agccttgtaa tttattcttt aataaatgtg ccggaagtaa
      481 tttgattatt tccgtttgct tttacaacat aatttcctgc aggtaaatga tggatatcta
      541 acgcaatttt attttgtgta ggataaacat tatttatttg cataacaaca tctcccatca
      601 tattaaatac ttcaatatta ttaacttcct tttcggctga taatgtaata ttgatgtaat
      661 tgtctgcagg atttggcgat aaatttaagg tattgtttaa cgcattattt atattaataa
      721 attcagttgc tttaaataac tctacagttc cgccttctcc accggtaact attatatctc
      781 caataaaatc ttgttgattt tgatcagcag cataataata acgatcgtgg aaaaacatgg
      841 gcacttctat ccattcttga acaacaaaat cataagcata agtgcctgaa aatgttaaat
      901 ctactccgaa accggacgca ttagttcctc ccggtactag catacatggc atatacacat
      961 cgtaatcaaa ccagtaaaag gcaaacgcct taatataact ttgtggttca ggaattgggt
     1021 cgaaatctac ccatgaatca ccggataaag ataatttttc tacactggta ataccttcat
     1081 aatcgaagcc attaaaa
//
LOCUS       DAORKW010000776         1096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_11529_length_1096_cov_4.097022, whole genome shotgun sequence.
ACCESSION   DAORKW010000776 DAORKW010000000
VERSION     DAORKW010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11529_length_1096_cov_4.097022"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1096
                     /locus_tag="PLB46_17760"
     CDS             <1..>1096
                     /locus_tag="PLB46_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78432.1"
                     /translation="LVVPGYQYPDDRTNCTDTDAFTIDVYNGDVIWDVPSCQEGIYNI
                     AILITEYRDGVRLGSILRDMEIFVEKTTNHAPEIAALNDTCIYAGEELNILVTATDPD
                     VTQTLTLTATGGPLYIDESPAEFFSTPNEGSVNGTFTWQTICNHIRSEVYSVVFKAED
                     DFQLGFEEIPLVDLETWLITVVAPPPIILDAVPTANEITITWQATYECASANNFLGFS
                     IWRKVGCDTTDFDKCQQGLDGTGYIQIGFVEDVYSFVDLAVSYGQQYAYRVVAEFGER
                     SDLAPDFIYNEVASAPSDAVCAELKRDLPIINHASVRTTSTTNGSVYVGWYKPLGTDL
                     DTTIFLPPYKYELLRYEGFTPARTPVSMAP"
BASE COUNT          315 a          211 c          231 g          339 t
ORIGIN      
        1 gtttggttgt tccgggttat caatatcctg acgaccgaac aaattgcaca gataccgatg
       61 catttaccat cgatgtttat aacggtgatg ttatttggga tgtaccctcg tgtcaggaag
      121 gtatttataa tattgccatt ttaatcacag aatatcgcga tggtgttcga ttggggagta
      181 ttttgcgcga catggaaatt tttgtagaaa aaacgactaa tcatgccccg gaaattgctg
      241 cattaaatga tacttgtatt tatgcaggtg aggaactgaa tatattagtc acagcaactg
      301 accccgatgt cacccaaacg ttaacactta ccgcaacagg cggaccactt tatattgatg
      361 agagccccgc agaatttttc agcacaccta atgaaggcag tgtaaacgga acattcacat
      421 ggcaaacaat atgcaaccat attcgttctg aagtatattc tgtggtgttt aaagcggaag
      481 atgattttca attgggtttt gaagaaattc ccttggtaga tttagaaaca tggctcatca
      541 ctgttgtagc acctccacca attattttgg atgctgtgcc aactgcaaat gaaataacca
      601 taacctggca ggcaacttat gaatgtgcca gtgcaaataa ttttctcgga ttttccattt
      661 ggagaaaagt aggttgtgat actactgatt ttgataaatg tcagcagggt ttagatggca
      721 ccggatatat tcaaatcggt tttgttgaag atgtatattc atttgtcgac ctcgcagtaa
      781 gttacggaca acaatatgct tatcgcgtag ttgctgaatt tggtgaacgc agcgatttag
      841 ctccggattt tatttataat gaagttgcaa gtgcgccatc tgatgcagta tgcgcagaat
      901 taaaacgcga tttacccatt attaatcacg ccagtgtgag aacaacttct accaccaatg
      961 gttctgttta tgttggctgg tataaaccac ttggcactga tttagatact actatttttc
     1021 ttccgccgta caaatatgaa ttactgcggt acgaaggatt taccccggct cgtacccctg
     1081 tatctatggc accttt
//
LOCUS       DAORKW010000777         1086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_11746_length_1086_cov_4.065955, whole genome shotgun sequence.
ACCESSION   DAORKW010000777 DAORKW010000000
VERSION     DAORKW010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11746_length_1086_cov_4.065955"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PLB46_17765"
     CDS             complement(<1..1062)
                     /locus_tag="PLB46_17765"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HRF78433.1"
                     /translation="MEWPNAMFRNRGDLTFDDVREEWGMYFPSTSYGAAYADFDNDGD
                     IDVFVSGGNTRGSSMHKNNGDGTFTKVTEGILNDSLALKGWGIAFGDYDNNAYTDIVI
                     AAPFGFAGITDGNKLLSNNGTGSFIRIDTSIICQGTAPYTIPSWSDYDHDGDVDCFIG
                     SGPANGTVAVDYLFKNQFKETGIANYFTRITEAPIGTDLVDGQIWNWIDYDNDGDLDA
                     YLTNYVGVSLGLGMPNNLYRNDAGIFTKMTAAEVGPIVSDIGLSLGSVWEDFDNDGDL
                     DCFVTKDGPAYCTYYENNNGVFTGITSEPITDFYASHYGATTGDYDKDGDVDLFVSAT
                     NPGKKLFINSASENGNKWIN"
BASE COUNT          338 a          243 c          172 g          333 t
ORIGIN      
        1 attaatccat ttattcccat tttcggatgc ggaattaata aataattttt tacccggatt
       61 tgttgcagaa acaaacaaat caacatcgcc atctttgtcg taatcgcctg tagttgcgcc
      121 ataatgtgat gcataaaaat cagttatagg ttcagatgtt attccggtaa aaacgccatt
      181 attattttcg taatatgtgc aataagcggg tccgtcttta gtgacaaaac aatctaaatc
      241 gccatcgtta tcaaaatctt cccaaaccga gcctaatgac aggccaatat cactcactat
      301 aggtcccact tctgcagctg tcattttagt aaatataccg gcatcatttc tatataaatt
      361 attcggcatt ccaagtccca aggatacgcc cacataattt gtcaaataag catctaaatc
      421 accatcatta tcataatcaa tccaattcca gatttgacca tcaactaaat cggttccaat
      481 tggtgcttca gtaatccgtg taaaataatt tgctatccct gtttctttaa attgattttt
      541 aaagagataa tcaacagcaa cagttccatt tgccggaccg gagccaataa aacaatctac
      601 atcaccatca tggtcgtaat cgctccatga aggaattgta tacggtgctg ttccctgaca
      661 aataattgaa gtatcgattc ggataaatga ccctgttccg ttattggaaa gtaatttatt
      721 gccatcagta attcctgcaa aaccaaaagg tgcagcaatt acgatatcgg tgtaggcatt
      781 attatcataa tcgccaaatg ctattcccca cccttttaat gcaaggctgt cgttgagtat
      841 gccctctgtt actttagtaa atgttccatc accgttattt ttatgcatag atgagccgcg
      901 agtatttcct cccgaaacaa atacatcgat atcgccatca ttatcaaaat cagcatatgc
      961 agcaccgtat gaagtggatg ggaaatacat accccattct tcgcgcacat catcaaaagt
     1021 taaatcacca cgattacgga acatcgcgtt cggccattcc aatactgagt tttccggtat
     1081 ttgttt
//
LOCUS       DAORKW010000778         1076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_11967_length_1076_cov_3.563173, whole genome shotgun sequence.
ACCESSION   DAORKW010000778 DAORKW010000000
VERSION     DAORKW010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11967_length_1076_cov_3.563173"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PLB46_17770"
     CDS             complement(<1..789)
                     /locus_tag="PLB46_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78434.1"
                     /translation="MKWRILILSAISLFFTACQVQENIQDGQTAYTLKKYKLAAELLQ
                     KDFNKAELPDEKSKIAFQIGESYANNNNYENAGEWYFKAYDLGYDSEAILKYALMLKA
                     QEKYEEALKEFEKYLLEEPYRRPEITLEMDACKNGIAWKQRQENEYERDTYVTEIITL
                     NSGDADFNPVIYQGDQLIFTSSRDVSTGENNDSWTGDKYYDLYVATIKDVNSFSAPEL
                     FKGPFNSAYNDGTLAFNKDYSEVYFTRCGTDDRKVDDYCGIYVSN"
BASE COUNT          322 a          219 c          163 g          372 t
ORIGIN      
        1 attgctcaca taaataccgc aataatcgtc cacctttctg tcatcagttc cgcaacgggt
       61 aaaatatact tcagaataat ctttattaaa tgcaagcgta ccgtcattgt aagcagaatt
      121 aaatggccct ttaaataatt ccggtgctga aaaactattt acatctttaa tggtagcaac
      181 atacaaatca taatatttgt cgccagtcca tgaatcatta ttttcacctg ttgacacatc
      241 gcgtgatgaa gtgaaaatta attgatcacc ctgatatatt accggattaa aatcggcatc
      301 acctgaattt aacgtaataa tttcggtaac ataagtatca cgttcgtatt cgttctcttg
      361 tcgctgtttc catgctatcc catttttaca ggcatccatt tccaacgtta tttccggacg
      421 gcgataaggt tcttccaaca aatatttttc gaattctttt aaggcctctt catatttttc
      481 ctgtgccttc aacatgaggg catatttcaa aatagcctca ctgtcgtaac ctaaatcata
      541 tgccttaaaa taccattctc ccgcattttc atagttattg ttattcgcat aactttctcc
      601 aatctgaaaa gctattttcg atttttcatc cggaagttca gccttgttga aatccttttg
      661 caataactca gcagcgagct tgtatttttt taatgtataa gctgtttgac cgtcctgtat
      721 gttctcctgc acctgacagg cagtaaaaaa caaactaatt gcacttaata tcagtattct
      781 ccacttcata aatatcaagt aaagttataa aacgaatata taacccgatt ggtatatctg
      841 tataagtttt gtaaaatctc catttcggct taatttcatc ataaatacag caaaaatcat
      901 tctatgaaac tattttttac ccttttagca gtcggaattt gtttttttgt tgcggcgcaa
      961 cctaaaattg acgtagtgct tgtagccgaa ggatttgacg aaccggtgga tattacacat
     1021 gccggtgatg agcgcttatt tatcgttgaa cgtttgggta aaatcaaaat tctata
//
LOCUS       DAORKW010000779         1062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_12302_length_1062_cov_6.265144, whole genome shotgun sequence.
ACCESSION   DAORKW010000779 DAORKW010000000
VERSION     DAORKW010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12302_length_1062_cov_6.265144"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PLB46_17775"
     CDS             <1..870
                     /locus_tag="PLB46_17775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HRF78435.1"
                     /translation="GTAFNWVENTVLNLNSHYINYSPDKPLACEAYVNIYTQPVGTAI
                     QTMYSVLLSNDGFYIPNNSEPVTDVASAFENGHGDDELFVWAMSSHTHQYGTDFNIYK
                     RTPDNEKGEQIFDGACGATNGVPGCLDEIYDYKHPPTRYWEGMLPIKWEDGIIYETTW
                     INDGPEPVGFGLTSDDEMMVMMYFFVDDTTGLNLPQTVDIEDIPEALQISVFPNPAKD
                     VFYIQSALNLSGAAVTLTDLKGSVVRTQLSESLTAGANYYQVDTYDLSPGLYLINILN
                     NEGVTVTEKIVIQ"
BASE COUNT          348 a          162 c          206 g          346 t
ORIGIN      
        1 ggaactgcat ttaactgggt agaaaataca gtgttgaatt taaattcaca ttatattaat
       61 tattccccgg ataaaccact tgcctgcgaa gcatatgtaa atatttatac gcaaccggtt
      121 ggaacggcaa tacagaccat gtattcggta ttattatcta acgatggatt ttatattccc
      181 aacaatagcg aaccggtcac ggatgttgcc agcgcatttg agaatggtca tggcgatgat
      241 gaattatttg tttgggcaat gagtagccat acacaccaat atggtaccga ttttaatatt
      301 tataaacgta cacctgataa tgaaaaaggt gaacaaatat ttgatggtgc ttgcggcgct
      361 actaatggtg tgccgggttg tttagatgaa atttatgatt acaaacatcc gccaactcgt
      421 tattgggaag gtatgttgcc aattaaatgg gaagatggta ttatatacga aacaacatgg
      481 attaacgatg gacctgaacc tgtaggattt ggtttaacca gcgatgatga aatgatggta
      541 atgatgtatt tttttgtaga tgatactaca ggattaaatt tacctcaaac tgttgatatt
      601 gaagatattc cggaagcatt acaaatttcg gttttcccaa atcctgcaaa ggatgtattt
      661 tatattcaga gtgctcttaa tttaagcggt gctgcagtta ctttaactga tttaaaagga
      721 agtgttgttc gcacacaact ttcggagtcg ttaactgcag gtgccaatta ttatcaggtt
      781 gatacttatg atttatcacc gggactttat ttgataaata ttttaaataa tgaaggcgta
      841 actgttactg aaaaaatagt aattcaataa actacaaaaa ttattttaac ccggattgca
      901 tttattaaaa atgtggtccg ggttttttta ttggcttaaa cttatttttt gaacagccgt
      961 aacgccggag catacagaga aaatacacgc aaagttcaca gagaattatt tcgtatattc
     1021 gaattaatta ctctggttat tcattaaaaa taactttaaa ca
//
LOCUS       DAORKW010000780         1046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_12723_length_1046_cov_4.403633, whole genome shotgun sequence.
ACCESSION   DAORKW010000780 DAORKW010000000
VERSION     DAORKW010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12723_length_1046_cov_4.403633"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..>1046)
                     /gene="serS"
                     /locus_tag="PLB46_17780"
     CDS             complement(60..>1046)
                     /gene="serS"
                     /locus_tag="PLB46_17780"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRF78436.1"
                     /translation="IAENIKNLLLTLPNLPQEIVPAGKDAEDNEVVRSGGDIPVFSFN
                     AQPHWELTEKYNLINFELGNKITGSGFPVYINKGARLQRALVAYFLDKAGEAGYIEYI
                     PPFMVNADSAYGTGQLPDKEGQMYHAVVDDFYLIPTAEVPVTNIYRDVIVKEDELPIK
                     MTAYSPCFRREAGSYGKDVRGLNRLHQFEKVEIVEIAHPDNSVERHETMVKHVENLVQ
                     ELGLPYRILRLCGGDMGFASAITYDFEVFSAAQQRWLEVSSVSNFETFQSNRLKCRFK
                     DSTDGKNKLVHTLNGSALALPRILAAILENNQTPEGINIPEVLWKYTGFKTI"
BASE COUNT          298 a          223 c          224 g          301 t
ORIGIN      
        1 aatggatggc cggttgggat ggataaggcg gagcggaatg ttgctgctcc aactattttt
       61 tatattgttt taaacccggt atatttccaa agcacttcag gaatgttgat gccttcgggc
      121 gtttgattat tttctaaaat agcagcaaga atacgcggca atgccagggc acttccattg
      181 agggtgtgca ctaatttatt tttaccatcg gtgctgtcct taaaacgaca ttttaagcga
      241 ttggactgga atgtttcgaa gttagaaaca gaacttactt ctaaccaacg ttgctgagca
      301 gcggaaaaca cttcaaaatc gtaagtaata gctgaagcaa aacccatatc gccaccgcat
      361 aaacgcaaaa tacggtaagg caaaccgagt tcttgaacca aattttctac atgtttaacc
      421 atggtttcgt ggcgctcaac ggaattgtcg gggtgagcaa tttcaacaat ttccactttt
      481 tcgaattggt gtaaacggtt taaaccgcgc acatctttac cataactacc ggcttcgcgg
      541 cggaaacaag gagagtaagc ggtcattttt attggcaact catcttcttt cacaatcaca
      601 tctctgtaaa tattggtaac aggcacttcg gcagttggaa tcaggtaaaa atcatcgaca
      661 acagcgtgat acatttggcc ttctttatca ggcaactgac cggttccgta agcagaatcg
      721 gcatttacca taaatggcgg gatatattca atataaccgg cttcacccgc tttatcgagg
      781 aaatacgcaa ctaatgcacg ttgtaaacga gcgcctttat taatatatac cgggaatccg
      841 ctaccggtta ttttattccc cagctcaaag ttgatgagat tatatttttc ggtcagctcc
      901 caatgtggtt gagcgttaaa actaaataca ggaatgtcgc cgccgctgcg cactacctca
      961 ttatcttcag catctttacc ggcaggaaca atttcctgtg gcaggttagg aagtgtgagc
     1021 agcaggtttt taatgttttc tgcaat
//
LOCUS       DAORKW010000781         1046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_12724_length_1046_cov_4.123108, whole genome shotgun sequence.
ACCESSION   DAORKW010000781 DAORKW010000000
VERSION     DAORKW010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12724_length_1046_cov_4.123108"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..>1046)
                     /gene="fbp"
                     /locus_tag="PLB46_17785"
     CDS             complement(117..>1046)
                     /gene="fbp"
                     /locus_tag="PLB46_17785"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HRF78437.1"
                     /translation="DISIAAKIVNREVNKAGLVDILGAQGSENMSGEAQQKLDVYANN
                     QFMSALMAGGEVCGFASEEEDNFISFNGAISKSGKYVVLIDPLDGSSNIDVNVSIGTI
                     FSIYRRLSLSGGATLEDFLQKGTEQAAAGYIIYGSSTMMVYTTGNGVNGFTLDPSIGE
                     FCLSHPGITIPNKGIIYSINEGNYVHFPEGVKRYIKYTQEEDLKTQRPYTSRYIGSLV
                     ADFHRNMLKGGVYLYPETAKSPKGKLRLLYECNPLAFIAEQAGGMASNGTKRILDIEP
                     KELHQKTPLYIGSSAMVEKIEEFLERHLELSRK"
BASE COUNT          304 a          239 c          194 g          309 t
ORIGIN      
        1 cgaaaatgaa agatgtgatg cattggcagt tgcagctgca gaaggtaatc gtttgacaat
       61 cgacgaatac tacgaaacca acgaattatt tagcaaagac taagcgcgtg ccgatattac
      121 tttctggaca actccaaatg acgctccaaa aactcctcta tcttttccac cattgcactt
      181 gagccgatat ataaaggtgt tttttgatgt aattctttag gttcaatatc cagaatacgt
      241 tttgtgccgt tggaagccat tcctcctgcc tgttcagcaa taaaggctaa tggattacat
      301 tcatacaata aacgcaactt gccttttggc gattttgccg tttcaggata gagataaacc
      361 cctcctttca gcatattgcg gtggaaatcg gcaactaatg agccaatgta tcgacttgta
      421 taaggtcgtt gcgttttgag gtcttcttcc tgggtatatt taatataacg cttcacaccc
      481 tcaggaaaat gcacgtagtt accttcatta atggagtaga taatgccttt gttcggtata
      541 gttatacccg ggtgcgacaa acaaaattcc ccaatagatg ggtctaatgt aaatccgttc
      601 acaccattac cggttgtata caccatcatg gttgatgaac cgtaaataat ataaccagct
      661 gctgcctgtt cggtcccttt ttgaagaaaa tcctccaaag tagcaccacc acttaggctc
      721 aaacggcggt aaatggagaa aatagtcccg attgacacgt taacatcaat attgctggaa
      781 ccatctaacg ggtcgattaa taccacatat tttcctgatt tggagatagc gccgttaaaa
      841 ctgatgaaat tatcttcttc ttcactggca aatccgcata cttcaccacc tgccatcagt
      901 gcactcataa attgattgtt agcgtaaaca tccagcttct gctgcgcttc accactcata
      961 ttttcgctgc cctgagcacc taaaatatca actaaaccgg ctttatttac ttcgcggttg
     1021 acaattttag cggcaatact gatatc
//
LOCUS       DAORKW010000782         1040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_12883_length_1040_cov_4.337056, whole genome shotgun sequence.
ACCESSION   DAORKW010000782 DAORKW010000000
VERSION     DAORKW010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12883_length_1040_cov_4.337056"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PLB46_17790"
     CDS             complement(<1..999)
                     /locus_tag="PLB46_17790"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRF78438.1"
                     /translation="MMKTYSSEHIKNVVLVGHSGSGKTTLAECMLFEAGALNRRGLVS
                     ENNTVSDYNELEHERGNSVFSTLMHAEWKDTKINIIDTPGMDDFVGEVIAAFKVADTG
                     LLVLNAAHGVEVGTELIWEYADHLKKPMILICNHVDEEKSDFDNTIEQAKERFGRKVT
                     VVQYPYNQGVDFNAIIDVHTMVMYQFGPDGGKPEKLPIPESEMAKAQQLHNELIEAIA
                     ENDEGLMEKFFEKGTLEEDEMVKGMMLSIRHHELFPVFVASAKHNMGSGRIMGFIDKC
                     CPSAAHMKPEKLTNGQDLPCNSSNPTIAFVFKTVSEPHLGDMSFFKVCSGKVTAGID"
BASE COUNT          286 a          225 c          171 g          358 t
ORIGIN      
        1 atcgataccg gctgtcactt ttccactaca cactttgaaa aaactcatgt cgcctaagtg
       61 cggttcgctc acggttttaa atacaaatgc gatagttggg ttcgatgaat tacatggcaa
      121 atcctgacca ttggtaagtt tttcaggttt catatgtgcc gctgaaggac aacatttatc
      181 gatgaaaccc ataatacgac cacttcccat attgtgtttt gctgaagcta caaatacggg
      241 aaataattca tggtggcgaa tagataacat catacctttc accatttcat cttcttccaa
      301 tgttcctttt tcgaaaaatt tctccattaa accttcatca ttttcggcaa ttgcttcaat
      361 taattcgttg tgtaattgtt gcgcttttgc catttctgat tccggtatcg gtaatttttc
      421 aggtttgcca ccatcgggac caaattgata cattaccatg gtgtgtacat caataattgc
      481 attaaaatct acaccttgat tatatggata ttgcaccacg gtaacttttc tgccaaatcg
      541 ttctttggct tgttctatcg tattatcaaa atcagatttt tcttcatcta catgattaca
      601 aataagtatc atgggttttt tgagatggtc ggcatattcc caaattaatt cggtgccaac
      661 ttcaacgccg tgcgctgcgt ttaaaactaa taaaccggta tctgcaactt taaatgctgc
      721 tattacttct cccacaaaat cgtccatacc cggagtatcg ataatattta ttttagtgtc
      781 tttccattcg gcatgcatta atgtactaaa aacactgttc cctcgttcat gttctaactc
      841 gttgtaatct gaaacggtgt tgttttcgga aactaatccg cggcggttta atgctccggc
      901 ttcaaacaac atgcattctg caagggttgt tttaccggag cctgaatgcc ccaccaaaac
      961 aacattttta atgtgttcgc tactgtaagt tttcatcatg tcctcctttg ttttagaact
     1021 gcaatataaa aaaataaatc
//
LOCUS       DAORKW010000783         1028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_13204_length_1028_cov_3.971223, whole genome shotgun sequence.
ACCESSION   DAORKW010000783 DAORKW010000000
VERSION     DAORKW010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13204_length_1028_cov_3.971223"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PLB46_17795"
     CDS             complement(<1..747)
                     /locus_tag="PLB46_17795"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78439.1"
                     /translation="MKQQMLRFFTGIICILSSTLLNAATPTISDTIDIRHITINLDIT
                     DYDDNTINGWAELDVKILVDNITMLPLDLLGLTVDSVIDNAGNHLLYSHIGEALQITL
                     PITFNTGDSTKIKVYYQGEPEHDGSWGGWYWSGDYAYQMGVGFDAIPHNFGRIWFPCF
                     DNFIERSTFRYEIKTPDNKSAYCGGILESETDNGDGTKSYIWNCNQPIPSYLASVAVS
                     TYTGWTDTYSGIEEEIPIVIAVKPEDSADLS"
     gene            768..>1028
                     /locus_tag="PLB46_17800"
     CDS             768..>1028
                     /locus_tag="PLB46_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78440.1"
                     /translation="MFGKIRQFLLIGLLMLQVNVIFAQVDDTTRFEGVITLDSFVLSA
                     VRNGFNVDDFVRFVEEDTTFHQAFRNLRQVPYNSSATVRMFID"
BASE COUNT          288 a          195 c          202 g          343 t
ORIGIN      
        1 agacaaatca gcagagtctt caggcttcac tgctattacg attggaattt cctcctctat
       61 ccccgaatat gtatcagtcc aaccggtata agtagaaaca gcaactgatg ctaaataact
      121 gggaatgggt tgattgcaat tccaaatata agatttcgtg ccatcgccat tatctgtttc
      181 actttctaaa attccaccgc aataggcact tttattatcc ggtgttttaa tttcataacg
      241 gaaagtactg cgttcaataa aattatcaaa acaaggaaac catattcttc cgaaattgtg
      301 tgggatggca tcaaaaccta cacccatttg ataggcatag tcgccactcc agtaccatcc
      361 accccaactg ccatcgtgtt caggttcgcc ctgataatat acttttattt tcgtggaatc
      421 acctgtatta aatgtgatag gaagtgtaat ctgtaatgct tcccctatgt gcgaatatag
      481 aaggtgatta cccgcattgt cgattacaga atctacggtt aaccccagca ggtccagtgg
      541 caacatggta atgttatcta ccagaatttt aacatctaat tcagcccaac cgtttatcgt
      601 attatcatcg taatcggtga tgtccagatt gatggtgata tggcgaatat ctatagtatc
      661 gctgattgtt ggtgttgcag cattcagcaa tgttgagctc aggatgcata ttattccggt
      721 aaaaaatctt aacatttgtt gcttcataat agtaatttta caggtatatg tttggtaaaa
      781 ttaggcaatt cctgcttatt ggcttgttaa tgcttcaggt taatgtcatt tttgctcaag
      841 tggatgacac aactcgtttt gagggtgtta ttacactcga ttcttttgtg ttgagtgcag
      901 tccgcaatgg gtttaatgtg gatgattttg tacgttttgt agaggaggat actacttttc
      961 atcaggcgtt tagaaattta cgtcaagttc cttataacag ttcggcgaca gttagaatgt
     1021 ttattgat
//
LOCUS       DAORKW010000784         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_5515
            NODE_13360_length_1023_cov_5.466942, whole genome shotgun sequence.
ACCESSION   DAORKW010000784 DAORKW010000000
VERSION     DAORKW010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867732
            Sequence Read Archive: SRR1106873
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:10:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,561
            CDSs (total)                      :: 3,529
            Genes (coding)                    :: 3,523
            CDSs (with protein)               :: 3,523
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 0 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13360_length_1023_cov_5.466942"
                     /isolate="AMR_MDS_5515"
                     /isolation_source="Municipal sewage wastewater treatment
                     plants"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="China"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PLB46_17805"
     CDS             complement(<1..1007)
                     /locus_tag="PLB46_17805"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRF78441.1"
                     /translation="MENGFMVEEAKGGVTYIDENTVIISTDFGPGSMTTSGYPSQAKI
                     WKRGTALKDAKLIYTGDINDVGAWGGVMRDGAKAYTIISRAITFYTSEAYVWMDNKAV
                     KLELPEDAESRGILNNQYIIALKSDWTVRGVTYKQGDLISLDFTELLKGVYQIQLIYR
                     PDEFSSISGVSVTKNKLLLELISNVNGELYVYTFSQNVWTKEKVKAPEYGKISVIDID
                     EFSDKYYFNFANFLTPTTLYYADAASGDVKPIKSLPAYFDGSKFKVEQFKATSADGTL
                     VPYFIVSSKTAANNGHNPTLLYAYGGFESSSLPFYSGTVGTAWLERGGVYVLANISRG
                     GG"
BASE COUNT          324 a          218 c          155 g          326 t
ORIGIN      
        1 cccccaccac gggaaatatt ggctaataca tatacaccac ctctttctaa ccaagccgtt
       61 ccaacggttc cggaataaaa aggtaaagaa gatgattcaa aaccaccgta tgcatataat
      121 aaggttggat tatgtccatt attagcagcc gtttttgaac taacaataaa atacggtacc
      181 aatgttccat ccgctgaagt tgctttaaat tgttctactt taaatttact gccatcaaaa
      241 taagccggca acgatttaat tggtttcaca tcacctgatg ctgcatcagc ataatataaa
      301 gttgtaggtg ttaagaagtt cgcgaaatta aaatagtatt tatctgaaaa ttcatcaata
      361 tcaataactg aaattttacc gtattcaggt gcctttactt tttctttcgt ccatacattt
      421 tgactaaacg tatacacata taattcgcca ttcacgttag aaattaattc gagtaacaat
      481 ttattttttg tcaccgacac acccgaaata ctggaaaatt catccgggcg ataaattaac
      541 tgaatttgat aaacaccttt taataattcg gtgaaatcaa gactgattaa atcgccttgt
      601 ttataggtaa cgccacgcac tgtccaatcc gattttaagg caataatata ttggttgttt
      661 aaaatacctc ttgattcagc atcttcaggt aattccagtt taactgcttt gttatccatc
      721 catacataag cctcagaagt ataaaatgta atagcacgcg aaataatagt gtatgctttc
      781 gcaccatcgc gcattacacc accccatgca cccacgtcgt taatatcacc ggtatatatc
      841 agctttgcat cttttaatgc agttcctctt ttccaaattt ttgcctgcga agggtaaccg
      901 gaagtggtca tagaaccggg accaaaatcg gtgctgataa ttacagtatt ttcatcaatg
      961 taggtaacac ctccttttgc ttcttccacc ataaatccat tttccacaaa ggctttttta
     1021 ttg
//
